cssi/SciSciGPT-SciSciNet huggingface dataset:
Satellite DNA-correlated nucleosomal proteins in Drosophila virilis.

Three major satellite DNAs comprise 40–45% of the genome of Drosophila virilis. Since these satellites are not substrates for most restriction enzymes, we were able to digest D. virilis nuclei with HaeIII and micrococcal nuclease and isolate chromatin fractions containing variable levels of satellite DNA. Electrophoretic analysis of these chromatin fractions revealed that the level of the acid-soluble chromosomal protein, cp17.3, was directly related to the percentage of satellite DNA in chromatin. The correlation between cp17.3 and satellite DNA abundance suggests that cp17.3 is involved in the heterochromatic condensation of satellite DNAs. cp17.3 occurs at a frequency of one molecule per 10–20 nucleosomes. It is detected in an electrophoretically distinguishable class of mononucleosomes, provisionally identified as MN1uH2A, which contains ubiquitinated histone H2A (uH2a) but lacks histone H1. It is not detected in MN1, a second class of mononucleosomes, which lacks uH2A and H1. Since cp17.3 is correlated with satellite DNAs and present in nucleosome cores, it might be a histone variant specifically associated with satellite DNAs.
Site Suppression and Pore Choking in the Deactivation of Nickel-Molybdenum Coal-Liquid Hydrotreatment Catalyst

Abstract Coal-liquid hydrotreatment (HT) catalysts deactivate by active site suppression and/or pore choking. The relative importance of these two mechanisms is quantitatively assessed by evaluation of the intrinsic reaction rate constant, kint, and Deff, the effective diffusivity, for catalysts removed at various age levels from a commercial HT unit. The two parameters are obtained from the observed rate constants for the hydrodesulfurization of thiophene as a function of temperature (between 180 and 550°C) using a modified version of the Constant-Deactivation Arrhenius Plot technique. At catalyst ages less than 200 lb. resid/lb. catalyst, pore choking is the dominant mechanism of deactivation. At higher catalyst ages, while both pore choking and active site suppression are responsible for catalyst deactivation, the contribution of pore choking is greater than the contribution of site suppression. These results do not change qualitatively with temperature.
Nitric oxide decreases IGF-1 receptor function in vitro; glutathione depletion enhances this effect in vivo

Insulin-like growth factor-1 (IGF) helps maintain healthy articular cartilage; however, arthritic cartilage becomes less responsive to the anabolic actions of IGF. We previously showed that high concentrations of nitric oxide (NO) decrease IGF receptor tyrosine phosphorylation and response to IGF in intact chondrocytes. The current studies evaluate direct effects of NO on IGF receptor kinase (IGF-RK) in vitro. NO from S-nitroso-N-acetyl-d,l-penicillamine (SNAP) or 1-hydroxy-2-oxo-3-(N-3-methyl-aminopropyl)-3-methyl-1-triazene (NOC-7) inhibits IGF-RK auto- and substrate phosphorylation in a dose and time dependent manner. There is a linear correlation between inhibition of auto- and substrate phosphorylation (r(2)=0.98). Increasing either dithiothreitol or reduced glutathione (GSH) content of the phosphorylation buffer to protect thiol groups blocks NO inhibition of IGF-RK substrate phosphorylation. Increased S-nitrosylation of cysteines in IGF-RK after exposure to SNAP suggests that NO may react with sulfhydryl groups, form S-nitrosothiols, which may result in functional modifications. NO blockade of IGF-1 stimulated proteoglycan synthesis in intact cells is enhanced when chondrocyte glutathione is depleted. The in vitro system shows that there can be direct effects of NO on IGF-RK that modify receptor function; the intact cell studies suggest that the mechanisms identified in vitro may be important in intact chondrocyte insensitivity to IGF-1 in cells exposed to NO.
A theoretical investigation of double potential step techniques as applied to the first order, one-half regeneration mechanism at planar electrodes: Chronoamperometry, chronocoulometry and chronoabsorptometry

Abstract Analytic solutions for the current, charge, and optical responses of a first-order “one-half” regeneration mechanism following double potential step excitation are reported. Polynomial approximations to the resulting working curves are developed.
Assembly and Cooperativity of Metabotropic Glutamate Receptors

Metabotropic glutamate receptors (mGluRs) are G-protein coupled receptors that are found throughout the nervous system where they respond to the major excitatory neurotransmitter, glutamate, to modulate synaptic transmission and plasticity via a variety of effectors. mGluRs are characterized by obligate dimerization and the presence of large extracellular ligand binding domains. In this study we used a combination of novel optical techniques to examine mGlu stoichiometry and to explore how these receptors cooperatively activate in response to ligand binding. We first used single molecule fluorescence photobleaching in live Xenopus oocytes and in receptors immobilized from mammalian cell lysate to map the dimer interfaces of homo and heterodimers. This work was complemented by experiments that use a covalently attached photoswitchable tethered ligand (PTL) that works as an agonist to analyze the cooperativity that arises from ligand binding to one or both subunits within a dimer. Finally, intersubunit FRET was used to analyze the conformational changes of mGluRs in order to gain an integrative view of receptor assembly, structural dynamics, and activation.
Ribosome formation in HeLa cells in the absence of protein synthesis

Ribosomal RNA synthesis and ribosome maturation in HeLa cells can occur when protein synthesis has been completely stopped by cycloheximide. This result implies the availability within the cell of every protein necessary to construct ribosomes. The ribosomes which are newly formed in cycloheximide enter into association with chains of mRNA.
Direct observation of salt effects on molecular interactions through explicit-solvent molecular dynamics simulations: differential effects on electrostatic and hydrophobic interactions and comparisons to Poisson-Boltzmann theory.

Proteins and other biomolecules function in cellular environments that contain significant concentrations of dissolved salts and even simple salts such as NaCl can significantly affect both the kinetics and thermodynamics of macromolecular interactions. As one approach to directly observing the effects of salt on molecular associations, explicit-solvent molecular dynamics (MD) simulations have been used here to model the association of pairs of the amino acid analogues acetate and methylammonium in aqueous NaCI solutions of concentrations 0, 0.1, 0.3, 0.5, 1, and 2 M. By performing simulations of 500 ns duration for each salt concentration properly converged estimates of the free energy of interaction of the two molecules have been obtained for all intermolecular separation distances and geometries. The resulting free energy surfaces are shown to give significant new insights into the way salt modulates interactions between molecules containing both charged and hydrophobic groups and are shown to provide valuable new benchmarks for testing the description of salt effects provided by the simpler but faster Poisson-Boltzmann method. In addition, the complex many-dimensional free energy surfaces are shown to be decomposable into a number of one-dimensional effective energy functions. This decomposition (a) allows an unambiguous view of the qualitative differences between the salt dependence of electrostatic and hydrophobic interactions, (b) gives a clear rationalization for why salt exerts different effects on protein-protein association and dissociation rates, and (c) produces simplified energy functions that can be readily used in much faster Brownian dynamics simulations.
CD146 expression and its close relationship to tumor progression in systemic malignancies besides gall bladder carcinomas.

Recent data suggest that CD146 may be involved in tumor development and may influence tumor prognosis in a number of systemic tumors besides gall bladder carcinomas. For instance, CD146 augments the development and progression of gastric carcinomas. It performs this function by accentuating epithelial mesenchymal transition (Liu et al., Int J Mol Sci 13:6399–6406, 2012). CD146 thus points towards a poor clinical outcome in gastric malignancies. An increase in gastric tissue vimentin is seen with an increase in CD146 levels. Similarly, around 8.7 % of lung adenocarcinomas express CD146. A decreased 5-year overall survival rate is seen following lung resection of pulmonary adenocarcinomas in male patients who express CD146 (Zeng et al., Proc Natl Acad Sci USA 109:1127–1132; 2012).
Properties of Low-Fat, Nonbreaded Pork Nuggets with Added Gums and Modified Starches

Low-fat (8%) pork nuggets were prepared with gums, modified food starches, and 90% pork. Sodium alginate with calcium-lactate or corn starch creme resulted in the greatest increases in cooking yield and tenderness. Convection oven cookery resulted in improvements in sensory and instrumental tenderness values compared with precooking and reheating. Nuggets with corn starch creme had notable distortions in shape after precooking and reheating. Gums and modified starches can improve tenderness in low-fat pork nuggets.
NITROGEN RETENTION IN THE BLOOD IN EXPERIMENTAL ACUTE NEPHRITIS OF THE CAT

It will be seen that uranium nephritis, which involves both tubules and glomeruli, the former more markedly than the latter, produces a marked accumulation of nitrogen in the blood. Chromate nephritis, which involves almost exclusively the tubules, produces only moderate retention of nitrogen. Cantharidin nephritis which involves both tubules and glomeruli, the latter more severely than does uranium, produces a marked accumulation of nitrogen, beginning early and persisting for a considerable period. The experiments were controlled by testing the blood of normal cats kept under the same conditions, these animals showing only slight variations from day to day. This general statement is in accordance with the physiological classification of these nephritides except that the retention in uranium occurs at an early stage, where, according to the physiological studies of Schlayer and his associates, and of Pearce, Hill, and Eisenbrey, the vascular changes have not as yet appeared. It must be noticed that in the three types of nephritis that form the subject of this investigation, anatomical study shows the glomerulus to be distinctly involved in the two forms where accumulation of nitrogen in the blood is most marked, a condition indicating that although almost pure tubular involvement produces only moderate accumulation, the additional involvement of the glomerulus is extremely important in leading to a retention of nitrogenous waste products. The accumulation of non-protein nitrogen in the blood and tissues is not large when compared with the total intake or elimination of nitrogen, and consequently it is practically impossible by means of ordinary nitrogen equilibrium experiments to demonstrate the fact of the retention, to say nothing of determining the degree of accumulation of waste products accompanying nephritis. That both can be demonstrated by the method employed in this research is clearly shown by the figures recorded ablove.
Purification of opioid receptor in the presence of sodium ions

Opioid receptor was solubilized from rat brain membranes with a mixture of the detergents CHAPS and digitonin in the presence of protease inhibitors and 1 M NaCl. The solubilized receptor bound mu-opioid agonists and antagonists with affinities similar to those of native membrane receptor. The affinity of solubilized receptor for the agonist PL017 was greatly reduced by GTP gamma S, suggesting the receptor is still associated with G-protein. The solubilized material was passed through an opioid antagonist (10cd) affinity column and a wheat germ agglutinin column, set up in series, to obtain a partially purified receptor preparation. This partially purified material bound mu-agonist with low affinity and the binding affinity was no longer affected by GTP gamma S. The partially purified receptor was further purified by repeating the affinity and lectin chromatography with smaller size column. Binding of opioid antagonist [3H]diprenorphine to the partially or purified receptors was dependent upon the presence of sodium ions. The purified receptor showed saturable and stereospecific binding for opioid ligands, was predominantly of the mu-type, and exhibited as a diffuse band with a medium molecular mass of 62 kD upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The average specific binding activity of the purified receptor was 18.8 +/- 2.3 pmol/micrograms protein, a value close to the theoretical estimation.
The painting of hand-coloured zoological illustrations

Millions of hand-coloured illustrations were incorporated in zoological books and journals between about 1710 and 1925. All the combined skills of the artists, etchers, engravers and lithographers, to produce good and accurate figures for these illustrations could be ruined by bad colouring. Yet we know the names of very few colourists who undertook this vital part of the publishing process. The identity of some of the British colourists, their working conditions and wages, and their method of working have been established from many scattered sources.
Aeroelastic stability predictions for a MW-sized blade

Classical aeroelastic flutter instability historically has not been a driving issue in wind turbine design. In fact, rarely has this issue even been addressed in the past. Commensurately, among the wind turbines that have been built, rarely has classical flutter ever been observed. However, with the advent of larger turbines fitted with relatively softer blades, classical flutter may become a more important design consideration. In addition, innovative blade designs involving the use of aeroelastic tailoring, wherein the blade twists as it bends under the action of aerodynamic loads to shed load resulting from wind turbulence, may increase the blade's proclivity for flutter. With these considerations in mind it is prudent to revisit aeroelastic stability issues for a MW-sized blade with and without aeroelastic tailoring. Focusing on aeroelastic stability associated with the shed wake from an individual blade turning in still air, the frequency domain technique developed by Theodorsen for predicting classical flutter in fixed wing aircraft has been adapted for use with a rotor blade. Results indicate that the predicted flutter speed of a MW-sized blade is slightly greater than twice the operational speed of the rotor. When a moderate amount of aeroelastic tailoring is added to the blade, a modest decrease (12%) in the flutter speed is predicted. By comparison, for a smaller rotor with relatively stiff blades the predicted flutter speed is approximately six times the operating speed. When frequently used approximations to Theodorsen's method are implemented, drastic underpredictions result, which, while conservative, may adversely impact blade design. These underpredictions are also evident when this MW-sized blade is analysed using time domain methods. Published in 2004 by John Wiley & Sons, Ltd.
Gram-negative Flagellin-induced Self-tolerance Is Associated with a Block in Interleukin-1 Receptor-associated Kinase Release from Toll-like Receptor 5

Flagellin from a number of Gram-negative bacteria induces cytokine and nitric oxide production by inflammatory cell types. In view of the evidence that flagellin responsiveness is subject to modulation, we explored the possibilities that a prior exposure to flagellin might result in a state of reduced flagellin responsiveness or tolerance and that lipopolysaccharide (LPS) and flagellin may induce a state of cross-tolerance to each other. Our results demonstrate that a prior exposure to flagellin results in a subsequent state of flagellin tolerance in human monocytes, THP1 cells, Jurkat cells, and COS-1 cells. Tolerance occurs within 2 h after addition of flagellin and does not require protein synthesis. Flagellin did not induce tolerance to LPS in monocytes and THP1 cells; however, LPS treatment of monocytes and THP1 cells resulted in a state of flagellin cross-tolerance. Flagellin-induced self-tolerance is not the result of a decrease in the steady-state level of toll-like receptor 5 (TLR5) or interleukin-1 receptor associated kinase (IRAK), but it is associated with a block in the release of IRAK from the TLR5 complex in flagellin-tolerant cells. Release is essential for IRAK activity because the TLR5-associated IRAK lacks kinase activity. LPS-induced cross-tolerance to flagellin is also associated with a block in IRAK release from TLR5. These results provide evidence for a novel mechanism of TLR signaling control.
Frequency division multiplexing technique for composite ambiguity function approximation

Abstract In this paper, we propose a frequency division multiplexing (FDM) diversity waveform technique to achieve a delay-Doppler response that approximates the composite ambiguity function (CAF) in a multiple target scenario. First, a channel estimate based on maximum likelihood estimation (MLE) for each subband is provided, and the signal to noise power ratio (SNR) needed to achieve the specific variance requirement of the MLE is derived. Next, using the Doppler transformation, we show how to combine the output response of each subband undergoing a different Doppler shift. Then the CAF approximation of each detected target is derived based on the coherent CLEAN algorithm. Finally, the CAF approximation of all the targets is attained, having the improved delay-Doppler resolution and thus providing a way to resolve initially undetected targets using a recursive procedure.
Genetically Determined Antigen of the Ne Subgroup of Gamma-Globulin: Detection by Precipitin Analysis

A genetic antigen, Gm(n), has been described for the Ne subgroup of gamma-globulin previously devoid of Gm factors. It was detected by precipitin tests with a primate antiserum to Ne-type heavy chains. A relation to the Gm(b) antigens of the Vi subgroup and the Gm(f) and Gm(y) antigens of the We subgroup was apparent. The availablity of genetic antigens for the heavy chains of three subgroups of gamma-globulin with varying relationships in different populations offers an approach to the mapping of the genes concerned.
Thromboembolic disease prophylaxis in total knee arthroplasty using intraoperative heparin and postoperative pneumatic foot compression

A prospective study was performed to assess the clinical efficacy of intraoperative heparin combined with postoperative pneumatic foot compression and aspirin for thromboembolic disease prophylaxis after primary total knee arthroplasty (TKA). This group of 48 patients all had a primary TKA performed under epidural anesthesia. The cohort of patients was compared with a similar cohort of patients matched for age and comorbidity. The control group consisted of 61 patients (41 unilateral patients, 20 bilateral patients) with 81 TKAs who received epidural anesthesia and postoperative pneumatic foot compression and aspirin. The same surgeon and anesthesiologist performed all cases. All patients had the pneumatic foot compression device applied in the recovery room and received 325 mg of enteric-coated aspirin twice a day beginning the night of surgery. Venography was performed on the fifth postoperative day for both groups to determine the incidence of deep vein thrombosis (DVT). In the control group with postoperative pneumatic foot compression (group 1), the overall incidence of DVT was 27% (22 of 81), with 10% (8 of 81) major calf DVT and no proximal (popliteal or femoral) DVT. In the study group with intraoperative heparin and postoperative pneumatic foot compression (group 2), the overall incidence of DVT was 25% (12 of 48), with 8% (4 of 48) major calf DVT and no proximal DVT. There was no statistically significant difference between groups 1 and 2 for the incidence of overall DVT (P > .05) or major calf DVT (P > .05). No complications were associated with the use of intraoperative heparin or the pneumatic compression device, and no patient developed a symptomatic pulmonary embolism up to 3 months postoperatively. Although this study confirms that pneumatic foot compression combined with aspirin is an effective form of DVT prophylaxis after TKA, the added benefit of intraoperative heparin was not statistically proven. Although greater statistical power is needed to determine whether intraoperative heparin provides an added benefit, this study reveals a low incidence of DVT in TKA with this protocol.
Approach and Avoidance Motivation: Investigating Hedonic Consumption in a Retail Setting

Abstract Retail shopping studies often conclude that desirable shopper behaviors, such as spending more money, indicate underlying approach motivation, while undesirable behaviors, such as leaving the store, indicate underlying avoidance motivation. However, hedonic consumption would seem to provide an opportunity not only for approaching fun and excitement but also for avoiding problems and stress in everyday life. This study investigates approach and avoidance motivations in a hedonic consumption context. Results show that both approach and avoidance motivation lead to heightened hedonic motivations for shopping and to more positive shopper evaluations. Additional investigation reveals several differences by gender and across four shopping contexts. Several theoretical and managerial implications are offered.
Computationally Designed Peptide Inhibitors of Protein-Protein Interactions in Membranes

We recently reported a computational method (CHAMP) for designing sequence-specific peptides that bind to the membrane-embedded portions of transmembrane proteins. We successfully applied this method to design membrane-spanning peptides targeting the transmembrane domains of the alpha IIb subunit of integrin alpha IIbbeta 3. Previously, we demonstrated that these CHAMP peptides bind specifically with reasonable affinity to isolated transmembrane helices of the targeted transmembrane region. These peptides also induced integrin alpha IIbbeta 3 activation due to disruption of the helix-helix interactions between the transmembrane domains of the alpha IIb and beta 3 subunits. In this paper, we show the direct interaction of the designed anti-alpha IIb CHAMP peptide with isolated full-length integrin alpha IIbbeta 3 in detergent micelles. Further, the behavior of the designed peptides in phospholipid bilayers is essentially identical to their behavior in detergent micelles. In particular, the peptides assume a membrane-spanning alpha-helical conformation that does not disrupt bilayer integrity. The activity and selectivity of the CHAMP peptides were further explored in platelets, comfirming that anti-alpha IIb activates wild-type alpha IIbbeta 3 in whole cells as a result of its disruption of the protein-protein interactions between the alpha and beta subunits in the transmembrane regions. These results demonstrate that CHAMP is a successful chemical biology approach that can provide specific tools for probing the transmembrane domains of proteins.
Analytic Reviews : Fluid Resuscitation in Head Injury

Head injury, either alone or in combination with hy povolemic shock, is the leading cause of traumatic death in this country. Factors contributing to mortality in clude the primary impact injury as...
Measuring atmospheric nutrient deposition to inland waters: Evaluation of direct methods

Several methodological problems have been noted in analyses of atmospheric phosphorus (P) and nitrogen (N) deposition to inland waters. These include the quantification of contamination, rates of dry deposition of nutrients on wet and dry surfaces, and short-range dust contamination. On and around a small lake in an agricultural region, we tested bulk and automated dry- and wet-deposition samplers for the presence of insect and dust contamination and presented procedures to eliminate biases. Local (≤100 m) dust deposition appeared negligible, so land-based measurements could be extrapolated to water; however, on- and near-shore samplers collected more insect contaminants than off-shore samplers. Insect contamination was large and most significant for estimates of P deposition, but a method to correct for it is proposed. We found that bulk and automated dryand wet-deposition samplers yielded similar values when contamination was eliminated. Screens for atmospheric deposition samplers were tested and led to lowered insect contamination and similar deposition rates to samplers without them. Bulk sampling can provide acceptable estimates of atmospheric nutrient deposition if contamination is quantified or excluded. A wet surface does not differ significantly from a dry surface for measurement of dry-fall of P. Short-range transport of dust-borne nutrients may be insignificant. Low-cost bulk samplers yielded accurate measurements of atmospheric nutrient deposition.
Selectively etched tunnel junction for lateral current and optical confinement in InP-based vertical cavity lasers

We demonstrate a thin, selectively lateral-etched, AlIn(Ga)As tunnel-junction (TJ) layer as a current and optical confinement aperture in the InP-based long-wavelength vertical cavity surface-emitting lasers (VCSELs). A high etch selectivity was demonstrated by etching the aperture a distance of several microns without affecting the surrounding InP etch-resistant layer. Edgeemitting lasers enclosing the TJ aperture showed high injection efficiency and low current spreading underneath the aperature. Single-mode continuous-wave operation of a 1.55-μm VCSEL was demonstrated successfully with a room-temperature differential efficiency of 21% using a 6-μm-wide TJ aperature.
THE SYNTAX AND SEMANTICS OF AS-PARENTHETICALS

This paper is a detailed investigation of the syntax and semantics of a single type of cross-linguistically common parenthetical expression, here dubbed As- parentheticals (e.g., Ames, as you know, was a spy). I show that a treatment of such clauses as adverbial modifiers combines with a motivated semantic analysis to account for a wide range of ambiguities concerning negation in particular, but also tense, modal, and adverbial operators. I provide a principled explanation for the impossibility of variable binding into, and extraction from, As-parentheticals, and argue that this construction yields novel support for the view (of Ladusaw 1992, and others) that negative DPs like no one are actually non-negated indefinites licensed by an abstract, clause-level negation. Overall, the analysis shows that parentheticals, in addition to being a rich source of puzzles in their own right, provide a useful probe into clause structure in general.
It’s All on Sale: Marketing Ethics and the Perpetually Fooled

Discussion of marketing deception has mostly focused on two main areas: first are cases that involve the intentional deception of people who tend to have compromised intelligence, such as children or the elderly, and second are cases that involve intentional falsehoods or the withholding of vital information, such as Madoff’s exploits. This article will differ from most in the field by examining marketing practices that are generally truthful, but deceive almost everyone. These practices do not fool just small select groups, but are fooling those usually assumed to be rational. For example, we love “free” merchandise so much that we are willing to irrationally settle for less to get the free product. Behavioral economists and psychologists are proving that, as Dan Ariely puts it, most all of us are “Predictably Irrational.” Is it wrong for marketers to take advantage of the mass’s foibles as it is wrong to take advantage of children? The article will look at some of the behavioral economists’ data, how that data affects the rational and ignorant person standards of marketing, and suggest the reflective rational person standard as a way to morally evaluate marketing techniques given this new data.
Insulin-like growth factor-I regulation of renal 25-hydroxyvitamin D-1-hydroxylase activity.

Controversy exists regarding the role of GH and insulin-like growth factor-I (IGF-I) in the modulation of calcitriol production. While their administration increases serum levels of 1,25-dihydroxyvitamin D, the mechanism remains unknown. Investigations have also implicated GH as a causal factor underlying renal 25-hydroxyvitamin D-1 alpha-hydroxylase activity [1(OH)ase] secondary to phosphate depletion. Thus, we investigated the effects of IGF-I on 1(OH)ase and the relationships between these actions and those of phosphate depletion. Our studies indicate that IGF-I administration to normal mice results in a dose-dependent (0-10 micrograms/h) increase in 1(OH)ase with maximum effects evident after 24 h, independent of changes in serum calcium, phosphorus, and glucose levels. Similarly, hormone administration to phosphate-depleted mice increases enzyme activity (6.06 +/- 0.96 vs. 13.97 +/- 1.67 fmol/mg.min) but to a level significantly greater than that achieved in normals (2.72 +/- 0.4 vs. 5.01 +/- 0.56 fm...
Linkage Map for the Asian Tiger Mosquito [Aedes (Stegomyia) albopictus] Based on SSCP Analysis of RAPD Markers

A linkage map of the Asian tiger mosquito [Aedes (Stegomyia) albopictus (Skuse)] was constructed in an F1 intercross by monitoring the segregation of randomly amplified polymorphic DNA (RAPD) markers analyzed for single-strand conformation polymorphisms (SSCP). We hypothesized that SSCP analysis would reveal point mutations in RAPD fragments that would then segregate as codominant rather than dominant markers which are typically revealed through routine RAPD analysis. Markers were mapped to individual chromosomes by testing for cosegregation with Sex (chromosome I) or a polymorphism at the a-GPD allozyme locus (chromosome II). All other markers that cosegregated were assigned to chromosome III. Six RAPD primers amplified 68 polymorphic markers that segregated in a Mendelian fashion and were mapped. Contrary to our hypothesis, no codominant SSCP polymorphisms were detected, but fractionation of RAPD products on polyacrylamide gels and detection through silver staining proved to be a sensitive technique that allowed us to identify more markers than the standard analysis of RAPD PCR products on agarose gels.
Stated Preferences for Components of a Personal Guidance System for Nonvisual Navigation

This article reports on a survey of the preferences of visually impaired persons for a possible personal navigation device. The results showed that the majority of participants preferred speech input and output interfaces, were will- ing to use such a product, thought that they would make more trips with such a device, and had some concerns about the cosmetic acceptability of a device and the use of a single or stereo headphone interface.
Controls on fallen leaf chemistry and forest floor element masses in native and novel forests across a tropical island

Litter chemistry varies across landscapes according to factors rarely examined simultaneously. We analyzed 11 elements in forest floor (fallen) leaves and additional litter components from 143 forest inventory plots systematically located across Puerto Rico, a tropical island recovering from large-scale forest clearing. We assessed whether three existing, independently-derived, landscape classifications (Holdridge life zone, remotely sensed forest type (leaf longevity combined with geology generalized to karst vs. non-karst), and plot-based measures of forest assemblage) would separate observed gradients. With principal component and regression analyses, we also tested whether climate-, landscape- (geology, elevation, aspect, percent slope, slope position, distance from coast), and stand-scale (tree species composition, basal area, density, stand age) variables explained variation in fallen leaf chemistry and stoichiometry. For fallen leaves, C, Ca, Mg, Na, and Mn concentrations differed by Holdridge life zone and C, P, Ca, Mn, Al, and Fe concentrations differed by forest type, where leaf longevity distinguished C and Ca concentrations and geology distinguished C, P, Ca, Mn, Al, and Fe concentrations. Fallen leaf C, P, Ca, and Mn concentrations also differed, and N concentrations only differed, by forest assemblage. Across several scales, fallen leaf N concentration was positively related to the basal area of putatively N2-fixing tree legumes, which were concentrated in lower topographic positions, providing for the first time a biological explanation for the high N concentrations of fallen leaves in these locations. Phosphorus concentrations in fallen leaves by forest assemblages also correlated with the basal area of N2-fixing legumes, and P and N concentrations decreased with mean age of assemblage. Fallen leaves from younger (<50 yr, 86% of the plots) and often novel forests had higher P, Fe, and Al and lower C concentrations and lower C/P and N/P ratios than fallen leaves from older forests, the latter due to a decrease in P rather than changes in N. These findings suggest that both N and P availability may currently be greater on the island than pre-deforestation, and substantiate the unique roles that state factors play in contributing to the spatial heterogeneity of fallen leaf chemistry.
Efficient System for Directed Integration into the Lactobacillus acidophilus and Lactobacillus gasseri Chromosomes via Homologous Recombination

An efficient method is described for the generation of site-specific chromosomal integrations in Lactobacillus acidophilus and Lactobacillus gasseri. The strategy is an adaptation of the lactococcal pORI system (K. Leenhouts, G. Venema, and J. Kok, Methods Cell Sci. 20:35–50, 1998) and relies on the simultaneous use of two plasmids. The functionality of the integration strategy was demonstated by the insertional inactivation of the Lactobacillus acidophilus NCFM lacL gene encoding β-galactosidase and of the Lactobacillus gasseri ADH gusA gene encoding β-glucuronidase.
Bayesian feedback detection for adaptive transmission systems

The paper considers adaptive transmission systems with imperfect feedback channels. It is shown that if the average SNR for the forward channel is known to the transmitter, the feedback detection can be modeled as a classical multiple hypotheses testing problem. Though maximum a posteriori detection is optimal compared to maximum likelihood (in the sense of minimum probability of error) from a feedback communication link viewpoint, the overall effect on adaptive system performance shows that ML detection could be better in the high SNR range. The MAP scheme is generalized to Bayesian detection by defining a cost function matrix, which assigns (unequal) weights to the feedback decision error probabilities. The result is close to ideal bit error rate (BER) performance with only a small drop in spectral efficiency.
The use of articles by monolingual Puerto Rican Spanish-speaking children with specific language impairment

The present investigation sought to evaluate patterns of article use in a group of monolingual Spanish-speaking children with specific language impairment (SLI). In particular, because of conflicting results reported in previous studies, it was of interest to discern specific types of nontarget responses and how these corresponded to what has been reported in other Spanish-speaking children with SLI. Eleven children with SLI and 11 age-matched peers participated in the study. Three different spontaneous speech samples were gathered from each child. In addition, an experimental task that assessed the children's use of articles with a variety of nouns was also administered to the children. The results of the study for both spontaneous speech and experimental data indicated that the children with SLI performed significantly poorer in their use of Spanish articles than their age-matched peers. Most of the nontarget responses consisted of omission of the target article. In contrast to a previous study by Restrepo and Gutierrez–Clellen, the children did not present with deficits in noun phrase gender agreement. The gender errors that were observed appeared to be due to difficulties accessing the correct article form and not due to deficits in knowledge of the gender agreement paradigm. Possible theoretical explanations were explored suggesting that both processing and linguistic explanations, in particular optionality of determiners, could explain the observed patterns. Reasons for cross-study differences in error patterns are suggested, including relative phonological skill and language learning environment.
The CW domain, a structural module shared amongst vertebrates, vertebrate-infecting parasites and higher plants

A previously undetected domain, named CW for its conserved cysteine and tryptophan residues, appears to be a four-cysteine zinc-finger motif found exclusively in vertebrates, vertebrate-infecting parasites and higher plants. Of the twelve distinct nuclear protein families that comprise the CW domain-containing superfamily, only the microrchida (MORC) family has begun to be characterized. However, several families contain other domains suggesting a relationship between the CW domain and either chromatin methylation status or early embryonic development.
Teacher Advocacy in Bilingual Programs

As a consequence of changes in federal and state policies in education, educators who believe in the value and importance of bilingualism find themselves in a contested environment where their notions of best practices for emergent bilinguals contradict those espoused in such policies. In this context, acts of advocacy that support bilingual practices become an important part of teachers' work. The purpose of this article is to identify what advocacy for bilingual learners would and could entail, using studies that have focused on the work of bilingual educators in bilingual programs. Drawing on conceptual work and a review of 30 empirical studies, the article describes how bilingual teacher advocacy is conceptualized in and beyond the classroom setting. It concludes with insights regarding the multifaceted, complex phenomenon of teacher advocacy and its relevance to future research and teacher preparation for emergent bilingual learners.
AGGREGATE EFFECTS OF MULTIPLE PREDATOR SPECIES ON A SHARED PREY

We evaluated the potential for emergent nonlinear effects of multiple pred- ators on mortality of a shared prey species by conducting experiments in cages in an old field. We examined interactions among three spider predators, Pisaurina mira, Phidippus rimator, and Rabidosa rabida, and a grasshopper prey Melanoplusfemurrubrum. The pred- ators have disparate hunting behaviors and are located in different parts of the vegetation canopy used by the grasshoppers, suggesting the potential for nonlinear effects. We ex- amined the effects of spider species singly and in all possible combinations using a sub- stitutive design in each of two years. Multiple predator effects on grasshopper mortality were generally the average of the corresponding single-species effects, indicating that the species effects were largely substitutable. This result indicates that it is reasonable to aggregate these predator species into a single functional unit, despite their disparate in- dividual effects on grasshopper prey, when modeling predator-prey interactions for this system.
Evidence that weakened centromere cohesion is a leading cause of age-related aneuploidy in oocytes

Aneuploidy arising early in development is the leading genetic cause of birth defects and developmental disabilities in humans. Most errors in chromosome number originate from the egg, and maternal age is well established as the key risk factor. Although the importance of this problem for reproductive health is widely recognized, the underlying molecular basis for age-related aneuploidy in female meiosis is unknown. Here we show that weakened chromosome cohesion is a leading cause of aneuploidy in oocytes in a natural aging mouse model. We find that sister kinetochores are farther apart at both metaphase I and II, indicating reduced centromere cohesion. Moreover, levels of the meiotic cohesin protein REC8 are severely reduced on chromosomes in oocytes from old mice. To test whether cohesion defects lead to the observed aneuploidies, we monitored chromosome segregation dynamics at anaphase I in live oocytes and counted chromosomes in the resulting metaphase II eggs. About 90% of age-related aneuploidies are best explained by weakened centromere cohesion. Together, these results demonstrate that the maternal age-associated increase in aneuploidy is often due to a failure to effectively replace cohesin proteins that are lost from chromosomes during aging.
A 0.0052 mm 2 COMPACT DIGITAL PLL IN 65 nm CMOS

A novel structure of digital phase locked loop (PLL) is presented in this paper. It uses digitally controlled oscillator (DCO) to generate the clock. At the beginning of each reference clock cycle, the DCO is fully reset and restarts to oscillate to prevent the long term jitter accumulation and increase the loop stability. It uses three types of digital delay control methods, including delay cell number adjust, delay cell load adjust and in cycle load adjust to digitally control the DCO output clock frequency, in order to get wider frequency range and smaller jitter. This digital PLL uses NAND gate as the basic delay cell of ring oscillator, which can completely reset DCO in a very short time. It uses binary search to achieve fast lock and uses shift chain to get better input clock jitter tolerance. This digital PLL has been silicon validated in GLOBALFOUNDRIES 65 nm Generic process. Its chip area is only 0.0052 mm2. In typical condition, DCO's frequency has a wide range between 550 MHz and 2.45 GHz. Its total power is around 1.4 mW when DCO's frequency is 1.8 GHz. This PLL can be locked very fast in 25 divided reference clock cycles, and its output clock jitter is around 18 ps.
A control scheme for improving multi-vehicle formation maneuvers

In this paper we present a control scheme for improving multiple vehicles formation maneuvering. The advantage of this control scheme is that it makes it easy to prescribe formation maneuvers, has stability guarantees and adds a type of robustness to maneuvering formations through the use of group feedback. For moving multiple vehicles in formation the added advantages of this control scheme are explored through simulation and hardware results using wheeled mobile robots.
Aerosol direct radiative effect at the top of the atmosphere over cloud free ocean derived from four years of MODIS data

A four year record of MODIS spaceborne data provides a new measurement tool to assess the aerosol direct radiative effect at the top of the atmosphere. MODIS derives the aerosol optical thickness and microphysical properties from the scattered sunlight at 0.55-2.1 microns. The monthly MODIS data used here are accumulated measurements across a wide range of view and scattering angles and represent the aerosol s spectrally resolved angular properties. We use these data consistently to compute with estimated accuracy of +/-0.6W/sq m the reflected sunlight by the aerosol over global oceans in cloud free conditions. The MODIS high spatial resolution (0.5 km) allows observation of the aerosol impact between clouds that can be missed by other sensors with larger footprints. We found that over the clear-sky global ocean the aerosol reflected 5.3+/-0.6W/sq m with an average radiative efficiency of 49+/-2W/sq m per unit optical thickness. The seasonal and regional distribution of the aerosol radiative effects are discussed. The analysis adds a new measurement perspective to a climate change problem dominated so far by models.
Neuropathologic Features and Grading of Alzheimer-Related and Sporadic CAA

This chapter will consider neuropathologic features of CAA, an approach to grading the severity of CAA using straightforward morphologic criteria, and consequences of CAA in the brain; especially cerebral hemorrhage. Specifically, we will evaluate the relationship between sporadic CAA and that related to Alzheimer disease, unique neuropathologie features of CAA-related brain hemorrhage, and introduce the concept of CAA-associated microangiopathies (CAA-AM, e.g. microaneurysms, secondary angiitis, etc.); lesions that may well be the proximate cause of brain hemorrhage. Approaches to studying CAA pathogenesis and CAA-AM will be discussed in relation to the basic biology of the cerebral microvessel wall and its components, aspects of which are taken up elsewhere in this volume.
Parallel machine learning approaches for reverse engineering genome-scale networks

Reverse engineering whole-genome networks from large-scale gene expression measurements and analyzing them to extract biologically valid hypotheses are important challenges in systems biology. While simpler models easily scale to large number of genes and gene expression datasets, more accurate models are compute intensive limiting their scale of applicability. In this talk, I will present our research on the development of parallel mutual information and Bayesian network based structure learning methods to eliminate such bottlenecks and facilitate genome-scale network inference. As a demonstration, we reconstructed genome-scale networks of the model plant Arabidopsis thaliana from 11,700 microarray experiments using 1.57 million cores of the Tianhe-2 Supercomputer. Such networks can be used as a guide to predicting gene function and extracting context-specific subnetworks.
EXPERIMENTAL EPIDEMIOLOGY OF TUBERCULOSIS : THE EFFECT OF A PRIMARY INFECTION ON CONTACT TUBERCULOSIS IN RABBITS

73 per cent of normal rabbits exposed for about 1 year to cage mates infected with tubercle bacilli of bovine type acquired a respiratory or alimentary tuberculosis, which was fatal in 50 per cent of the cases. 63.6 per cent developed tuberculosis during the first 6 months. Of rabbits vaccinated with tubercle bacilli of human type and exposed in the same cages at the same time only 36.8 per cent acquired tuberculosis during the first 6 months. Later this resistance waned, and by the end of the year altogether 60 per cent had developed tuberculosis, of which 38 per cent succumbed. The disease in the vaccinated rabbits was shown to be of exogenous origin by the isolation in pure culture from the same rabbit of the human type bacillus from the primary infection, and of the bovine type bacillus from the naturally acquired lesion. The vaccination reduced the incidence, extent, and mortality of the disease, affected the route of infection, changed its pathological character, and retarded its progress. The disease acquired by vaccinated rabbits shared many characteristics with adult type tuberculosis in man. It is suggested that this method may be used with relative ease in studying many phases of naturally acquired tuberculosis in small laboratory animals.
Space group symmetry fractionalization in a chiral kagome Heisenberg antiferromagnet

The anyonic excitations of a spin liquid can feature fractional quantum numbers under space group symmetries. Detecting these fractional quantum numbers, which are analogs of the fractional charge of Laughlin quasiparticles, may prove easier than the direct observation of anyonic braiding and statistics. Motivated by the recent numerical discovery of spin-liquid phases in the kagome Heisenberg antiferromagnet, we theoretically predict the pattern of space group symmetry fractionalization in the kagome lattice SO(3)-symmetric chiral spin liquid. We provide a method to detect these fractional quantum numbers in finite-size numerics which is simple to implement in the density matrix renormalization group. Applying these developments to the chiral spin liquid phase of a kagome Heisenberg model, we find perfect agreement between our theoretical prediction and numerical observations.