cssi/SciSciGPT-SciSciNet huggingface dataset:
Expression of human recombinant β2-microglobulin by Aspergillus nidulans and its activity

The light chain of HLA class I protein (beta 2m) has been expressed in Aspergillus nidulans. The cDNA of beta 2m was modified using the polymerase chain reaction to include overlapping extensions for its subsequent fusion into an Aspergillus vector. This fusion resulted in beta 2m cDNA being flanked by the Aspergillus awamori glucoamylase promoter and the Aspergillus niger glucoamylase terminator. Expression of beta 2m was induced by the addition of starch to the culture medium. In preliminary mass culture trials, 177 micrograms/liter of f beta 2m were obtained in 60-liter fermentations. N-terminal sequencing of purified human beta 2m produced in fungi (f beta 2m) revealed that 28% of the purified protein was of proper sequence and 61% of the protein had an additional serine and lysine residue derived from the C-terminus of the fungal leader. Purified f beta 2m from culture supernatants appeared biochemically similar to beta 2m obtained from human urine (u beta 2m) as seen in immunoblot analysis. Functionally, f beta 2m effectively interacted as a subunit of class I MHC molecules. This was seen both in a sandwich ELISA for detecting properly folded HLA class I heavy chain and in assays showing cell-surface beta 2m exchange into the mouse class I MHC H-2Kd. In these experiments the biological activity of f beta 2m was indistinguishable from u beta 2m. The successful expression of biologically active beta 2m in A. nidulans suggests that fungal systems might be useful for the production of other active components of the HLA class I MHC complex.
Binding specificity of a monoclonal anti-DNA antibody☆

To investigate the interaction of DNA and anti-DNA antibodies in the immune complex disease of systemic lupus erythematosus, the fine specificity of binding of a monoclonal anti-DNA antibody was determined. This antibody, termed Cll, was derived from the fusion of spleen cells from an autoimmune MRL-lpr/lpr mouse with the myeloma cell line M45. In a solid-phase ELISA assay to measure anti-DNA activity, Cll showed preference for single stranded compared to double stranded DNA of animal origin. The Cll antibody also bound some deoxyribohomopolymers as well as ribohomopolymers, but failed to bind synthetic DNA duplexes. Defined size oligonucleotides with a size range of 2-(12-18) failed to inhibit the binding of Cll to single stranded DNA. This pattern of binding is consistent with the recognition of a unique structural determinant that can be represented by a variety of nucleic acids. The absence of antigenic activity among the oligonucleotides suggests that an extended polynucleotide structure is required for antibody binding, possibly because of a bivalent or 'monogamous' mode of interaction. The binding properties of Cll further suggest that its ability to participate in immune complex formation may be limited by the nature of the available DNA antigen.
Using parent-infant dyads as models for interpreting the parallel process

Studies indicate that parents replicate with the therapist the behaviors and perceptions they experience with their infants. During parent-infant therapy, the therapist may validate perceptions by comparing the parent's behaviors with verbal disclosures. A similar phenomenon occurs in psychotherapeutic supervision. Therapists in training tend to replicate with the supervisor the interactions of their patients, a phenomenon labeled parallel process.This paper suggests that parent-infant interaction may provide insight into the therapistpatient and therapist-supervisor relationships. Understanding the parallel process that occurs during parent-infant therapy may help the therapist explore the patient's perceptions and resolve conflict.
Dynamic modeling of grid integrated solar and battery energy system for cloud transient impact study

To assess the impact of intermittency of rapidly increasing solar photovoltaic (PV) generation on the grid, this paper presents the dynamic models of PV generation system, the battery energy storage system (BESS) and their associated control systems. The interfaces between these models and the mains grid are also provided. Using such dynamic models, the cloud transient impacts at different penetration levels of PV generation on the system responses are investigated using a simplified simulation approach proposed in this paper.
Congenital cardiac malformations in Adams-Oliver syndrome

Two patients with Adams-Oliver syndrome and congenital cardiac malformations are described. A literature review revealed a 13.4% occurrence of congenital cardiac malformations in individuals with Adams-Oliver syndrome, suggesting that cardiac anomalies are a frequent manifestation of this syndrome. All patients with Adams-Oliver syndrome (aplasia cutis congenita and terminal digital anomalies) should be evaluated for cardiac anomalies.
Ecstasy and Agony: Activation of the Human Amygdala in Positive and Negative Emotion

Considerable evidence indicates that the amygdala plays a critical role in negative, aversive human emotions. Although researchers have speculated that the amygdala plays a role in positive emotion, little relevant evidence exists. We examined the neural correlates of positive and negative emotion using positron emission tomography (PET), focusing on the amygdala. Participants viewed positive and negative photographs, as well as interesting and uninteresting neutral photographs, during PET scanning. The left amygdala and ventromedial prefrontal cortex were activated during positive emotion, and bilateral amygdala activation occurred during negative emotion. High-interest, unusual photographs also elicited left-amygdala activation, a finding consistent with suggestions that the amygdala is involved in vigilance reactions to associatively ambiguous stimuli. The current results constitute the first neuroimaging evidence for a role of the amygdala in positive emotional reactions elicited by visual stimuli. Although the amygdala appears to play a more extensive role in negative emotion, it is involved in positive emotion as well.
Video streaming with diversity

Packet path diversity is one of the recent advances in network-adaptive video streaming. In this paper we first examine two specific techniques for video streaming that exploit diversity to achieve improved performance. The first technique uses a framework for rate-distortion optimized scheduling of the packet transmissions over the available network paths. The second technique exploits feedback and channel probing to determine which network path should be used for transmission and to adapt the source encoding of the video to mitigate error propagation effects. In the final section, we compare the performance of these two techniques by analyzing experimental results and discuss their respective advantages and drawbacks.
C-reactive protein is associated with psychological risk factors of cardiovascular disease in apparently healthy adults.

Objective: The current study examined the relation of anger, hostility, and severity of depressive symptoms, alone and in combination, to C-reactive protein (CRP) in healthy men and women. Methods: A high sensitivity enzyme linked immuno sorbent assay (ELISA) was used to evaluate CRP levels in a multiethnic sample of 127 healthy, nonsmoking men and women. Fasting blood samples were collected the same day the assessments were done of anger and hostility using the Buss-Perry Aggression Questionnaire (BPAQ) and depressive symptomatology using the Beck Depression Inventory (BDI). A psychological risk factor (PRF) score representing a composite summary indicator of BDI and BPAQ-anger and -hostility was generated using principal component analysis. Log-transformed CRP values were examined using univariate and multivariate analyses adjusting for control variables of age, gender, body mass index (BMI), alcohol use, exercise frequency, ratio of total to high-density lipoprotein cholesterol, and family history of premature coronary heart disease (CHD). Results: Log-normalized CRP was correlated with BDI (r 0.21, p .02) and BPAQ anger (r 0.20, p .02), but not with BPAQ hostility. After adjustment for control variables, BDI ( 0.05, p .011), BPAQ anger ( 0.05, p .007), and the PRF composite score ( 0.27, p .005), but not BPAQ hostility ( 0.03, p .11), were significantly associated with log-normalized CRP. Conclusions: Greater anger and severity of depressive symptoms, separately and in combination with hostility, were significantly associated with elevations in CRP in apparently healthy men and women. These associations were independent of potential confounding factors. Key words: C-reactive protein, anger, hostility, severity of depressive symptoms, men, women.
Non‐Routine News and Newswork: Exploring a What‐a‐Story

Although newswork is usually accomplished through routine practices, some occurrences are far from routine. This study looks at how newsworkers at one local television station created their coverage of a major (“what-a-story”) story. Data came from newsroom observation. To manage this non-routine occurrence, newsworkers improvised around well-established routines, rather than developing an entirely different work process. Typifying likely story elements and story actors was a key strategy underlying their efforts to negotiate the status of the occurrence and to create news stories.
Molecular Motion at Soft and Hard Interfaces: From Phospholipid Bilayers to Polymers and Lubricants

Spatially resolved and time-resolved understanding of complex fluid situations compose a new frontier in physical chemistry. Here we draw attention to the significance of spatially resolving systems whose ensemble average differs fundamentally from the spatially resolved individual elements. We take examples from the field of fluid phospholipid bilayers, to which macromolecules adsorb; the field of polymer physics, when flexible chains adsorb to the solidliquid interface; and from the field of lubrication, when two solids are squeezed close together with confined fluid retained between them.
Hypersensitivity to heavy water: A new conditional phenotype

Wild-type strains of the yeast S. cerevisiae can grow on media containing 90% D2O. Using chemical mutagenesis we obtained a number of strains that grow on H2O-containing media but not on otherwise identical media containing 90% D2O. The frequency of these D2O-sensitive (d) mutants is comparable to the frequency of conventional temperature-sensitive (ts) mutants in the same mutagenized sample, and the ds mutations are distributed over a large number of complementation groups. Furthermore, most ds mutants do not display other conditional phenotypes, such as heat, cold, or osmotic sensitivity. Conversely, of 17 cell division cycle ts mutants tested, only 2 are also ds. Thus, the ds technique should be useful for producing conditional mutations in genes that are not amenable to the ts and cs approaches, and also for generating alternative conditional (ds) alleles in many other genes. In addition, the ds technique should make it possible to generate conditional (ds) mutants in homeothermic animals, thereby extending the advantages of conditional phenotypes to mammalian and avian genetics.
Angiographic and pathologic features of probable primary carcinoid-like hepatic tumors

The angiographic and pathologic features of two cases of primary carcinoid-like hepatic tumors are described. Neither patient had the carcinoid syndrome.
Quality control and improvement for multistage systems: A survey

A multistage system refers to a system consisting of multiple components, stations or stages required to finish the final product or service. Multistage systems are very common in practice and include a variety of modern manufacturing and service systems. In most cases, the quality of the final product or service produced by a multistage system is determined by complex interactions among multiple stages—the quality characteristics at one stage are not only influenced by local variations at that stage, but also by variations propagated from upstream stages. Multistage systems present significant challenges, yet also opportunities for quality engineering research. The purpose of this paper is to provide a brief survey of emerging methodologies for tackling various issues in quality control and improvement for multistage systems including modeling, analysis, monitoring, diagnosis, control, inspection and design optimization.
Increased N‐acylphosphatidylethanolamine biosynthesis in elicitor‐treated tobacco cells

Previous studies with tobacco (Nicotiana tabacum L.) cell suspensions indicated that elicitation of defense response (production of phytoalexins) with xylanase (1,4-β-D-xylanxylanohydrolase; EC 3.2.1.8) resulted in a dramatic acylation of phytosterols (Moreau et al. 1994). N-acylphosphatidylethanolamine (NAPE), an acylated derivative of phosphatidylethanolamine (PE), was recently demonstrated to be synthesized in vivo in plant tissues (Chapman and Moore 1993a). Here we report that acylation of PE was increased in elicitor-treated cells. NAPE levels increased 3-fold (from 1.6 to 4.8 mol% of total phospholipids) after a 2-h treatment of cell suspensions with xylanase (1 μg ml -1 ). Specific activity of NAPE synthase increased in parallel with NAPE levels. Levels of NAPE and NAPE synthase activity declined during the period of 2-4 h after elicitation while levels of acylated sterolglycosides (ASG) continued to increase. Radiolabeling studies with [2- 14 C]-ethanolamine confirmed that three times as much NAPE was synthesized in elicitor-treated cells compared to that in unelicited cells. Patterns of incorporation of [1- 14 C]-palmitic acid into membrane phospholipids in elicitor-treated cells suggested that increased acylation of lipids may be a result of changes in the acyl-coenzyme A pool. Treatment of cells with purified ethylene biosynthesis-inducing xylanase (EIX ; 1 μg ml -1 cells) resulted in increased levels of NAPE synthase activity comparable to those observed with the commercial preparations of xylanase. Boiled xylanase did not elicit an increase in the specific activity of NAPE synthase. Collectively our results demonstrate that the accumulation of NAPE in tobacco cells is attributable to increased activity of NAPE synthase. This suggests that NAPE may be specifically synthesized to play a protective role in membranes of plant cells as has been suggested for membranes of damaged animal cells.
A Two-Phase Set Partitioning Model for Passenger Transportation

A two-phase set partitioning model is proposed to solve passenger transportation, this problem is a real life application for the pickup and delivery problem where vehicles have to transport passengers from their locations to different destinations with minimal routing cost. Following the classical cluster-first route-second approach the problem is modelled in two phases and solved like a set partitioning problem using ant computing.
Mental Rotation in Human Infants: A Sex Difference

A sex difference on mental-rotation tasks has been demonstrated repeatedly, but not in children less than 4 years of age. To demonstrate mental rotation in human infants, we habituated 5-month-old infants to an object revolving through a 240° angle. In successive test trials, infants saw the habituation object or its mirror image revolving through a previously unseen 120° angle. Only the male infants appeared to recognize the familiar object from the new perspective, a feat requiring mental rotation. These data provide evidence for a sex difference in mental rotation of an object through three-dimensional space, consistently seen in adult populations.
Heat Shock Transcription Factor 1 Is Activated as a Consequence of Lymphocyte Activation and Regulates a Major Proteostasis Network in T Cells Critical for Cell Division During Stress

Heat shock transcription factor 1 (HSF1) is a major transcriptional regulator of the heat shock response in eukaryotic cells. HSF1 is evoked in response to a variety of cellular stressors, including elevated temperatures, oxidative stress, and other proteotoxic stressors. Previously, we demonstrated that HSF1 is activated in naive T cells at fever range temperatures (39.5°C) and is critical for in vitro T cell proliferation at fever temperatures. In this study, we demonstrated that murine HSF1 became activated to the DNA-binding form and transactivated a large number of genes in lymphoid cells strictly as a consequence of receptor activation in the absence of apparent cellular stress. Microarray analysis comparing HSF1(+/+) and HSF1(-/-) gene expression in T cells activated at 37°C revealed a diverse set of 323 genes significantly regulated by HSF1 in nonstressed T cells. In vivo proliferation studies revealed a significant impairment of HSF1(-/-) T cell expansion under conditions mimicking a robust immune response (staphylococcal enterotoxin B-induced T cell activation). This proliferation defect due to loss of HSF1 is observed even under nonfebrile temperatures. HSF1(-/-) T cells activated at fever temperatures show a dramatic reduction in cyclin E and cyclin A proteins during the cell cycle, although the transcription of these genes was modestly affected. Finally, B cell and hematopoietic stem cell proliferation from HSF1(-/-) mice, but not HSF1(+/+) mice, were also attenuated under stressful conditions, indicating that HSF1 is critical for the cell cycle progression of lymphoid cells activated under stressful conditions.
Enterprise strategies for reducing the life-cycle energy use and greenhouse gas emissions of personal computers

This paper examines the effectiveness of several important enterprise strategies for reducing the life-cycle energy use and greenhouse gas (GHG) emissions of office computers. A modeling framework is presented, which quantifies the annual primary energy use and GHG emissions necessary to maintain and operate an enterprise personal computer (PC) stock. The case of a California-based enterprise with 5,000 desktop PCs is considered as a baseline scenario. The model is applied to estimate technically-achievable reductions in life-cycle energy use and GHG emissions associated with six common enterprise PC management strategies, as compared to the baseline scenario. The total technical potential for primary energy savings is estimated at roughly 60%; the total technical potential for GHG savings is estimated at roughly 35%.
A YEN FOR BUSINESS - LANGUAGE-LEARNING FOR SPECIFIC PURPOSES - A JAPANESE EXAMPLE

The following paper chronicles the steps taken in developing a curricular project to design, implement, and evaluate a simulation business course for beginners learning Japanese at the university level. The steps in developing the program, from the selection of materials to the evaluation of the entire project, are presented together with insights gained from the experience and suggestions for improvement. The paper concludes with issues that are still unresolved and new directions to explore.
Data gathering algorithms in sensor networks using energy metrics

Gathering sensed information in an energy efficient manner is critical to operating the sensor network for a long period of time. The LEACH protocol presented by Heinzelman et al. (2000) is an elegant solution where clusters are formed to fuse data before transmitting to the base station. In this paper, we present an improved scheme, called PEGASIS (power-efficient gathering in sensor information systems), which is a near-optimal chain-based protocol that minimizes energy. In PEGASIS, each node communicates only with a close neighbor and takes turns transmitting to the base station, thus reducing the amount of energy spent per round. Simulation results show that PEGASIS performs better than LEACH. For many applications, in addition to minimizing energy, it is also important to consider the delay incurred in gathering sensed data. We capture this with the energy /spl times/ delay metric and present schemes that attempt to balance the energy and delay cost for data gathering from sensor networks. We present two new schemes to minimize energy /spl times/ delay using CDMA and non-CDMA sensor nodes. We compared the performance of direct, LEACH, and our schemes with respect to energy /spl times/ delay using extensive simulations for different network sizes. Results show that our schemes perform 80 or more times better than the direct scheme and also outperform the LEACH protocol.
Nonattainder as a Liberty Interest

Existing constitutional doctrine does not deal well with government blacklists, such as the highly publicized federal No Fly List. Prior blacklisting cases have been inconsistent in approach and result. The most commonly used theories ask whether blacklists impose a deprivation of liberty without due process, but there is no consensus as to what kind of liberty is at stake. The problem arises in part from an unduly constricted conception of liberty as protecting the things that people want to do (call them privileges), without considering that it also implicates ways that people do not want to be treated (call them immunities).This Article proposes that the constitutional immunity from bills of attainder - that is, the rule against singling out persons for punishment without trial - should be recognized as a due process liberty interest. This proposal has two major benefits. First, it provides a legal remedy currently unavailable to persons on government blacklists. Second, it offers coherence across lines of cases that have not previously been considered together. In particular, the proposal rationalizes the much criticized “stigma-plus” doctrine, which is best understood as a false start toward the protection of nonattainder as a liberty interest.
Thermal Transport Studies in Deepsea Sediment

Thermal problems associated with the emplacement of radioactive wastes in the deepsea sedimentary layer have been studied. In particular, the nature of the temperature field surrounding and the interstitial water velocity arising from a buried cask have been examined. For moderately low levels of heat generation by the decaying radionuclides, conservative estimates indicate that the velocity field will be extremely weak.1 Because of the low interstitial water velocity, thermal conduction models can be used to predict the temperature field in the surrounding sediments as well as the cask surface temperature. This is equivalent to “decoupling” the energy and momentum conservation relationships thus simplifying the solution of the temperature field.
Forkhead transcription factor FoxF1 interacts with Fanconi anemia protein complexes to promote DNA damage response

Forkhead box F1 (Foxf1) transcription factor is an important regulator of embryonic development but its role in tumor cells remains incompletely understood. While 16 proteins were characterized in Fanconi anemia (FA) core complex, its interactions with cellular transcriptional machinery remain poorly characterized. Here, we identified FoxF1 protein as a novel interacting partner of the FA complex proteins. Using multiple human and mouse tumor cell lines and Foxf1+/- mice we demonstrated that FoxF1 physically binds to and increases stability of FA proteins. FoxF1 co-localizes with FANCD2 in DNA repair foci in cultured cells and tumor tissues obtained from cisplatin-treated mice. In response to DNA damage, FoxF1-deficient tumor cells showed significantly reduced FANCD2 monoubiquitination and FANCM phosphorylation, resulting in impaired formation of DNA repair foci. FoxF1 knockdown caused chromosomal instability, nuclear abnormalities, and increased tumor cell death in response to DNA-damaging agents. Overexpression of FoxF1 in DNA-damaged cells improved stability of FA proteins, decreased chromosomal and nuclear aberrations, restored formation of DNA repair foci and prevented cell death after DNA damage. These findings demonstrate that FoxF1 is a key component of FA complexes and a critical mediator of DNA damage response in tumor cells.
RAM BIST

This paper describes a random access memory (RAM, sometimes also called an array) test scheme that has the following attributes: it can be used in both built-in mode and off chip/module mode; it can be used to test and diagnose naked arrays; fault diagnosis is simple and is "free" for some faults during test; it is never subject to aliasing; depending upon the test length, it can detect many kinds of failures; like stuck-cells, decoder faults, shorts, pattern-sensitive, etc; if used as built-in feature, it does not slow down the normal operation of the array; it does not require storage of correct responses; a single response bit always indicates whether a fault has been detected; the storage requirement for the implementation of the test scheme is zero; if used as a built-in feature, the hardware overhead is very low.
A thermodynamic analysis ofβ′-PdIn

A recently developed model for the thermodynamic properties of intermetallic B2 phases with triple defects is applied to Β′-PdIn. The values of the disorder parameter α, obtained independently from measurements of the activity and the partial enthalpy, are in reasonable agreement. The validity of a theoretical expression, which describes the temperature dependence of the disorder parameter, is confirmed experimentally. It is shown that the equations by Libowitz for triple defect B2 phases can be reduced to the less cumbersome, yet continuous, expressions by Neumann, Chang, and Lee.
The Incidence of Durotomy and its Clinical and Economic Impact in Primary, Short-segment Lumbar Fusion: An Analysis of 17,232 Cases.

STUDY DESIGN Retrospective database analysis. OBJECTIVE To determine the incidence of durotomy in primary short-segment lumbar fusion and assess its clinical and economic impacts. SUMMARY OF BACKGROUND DATA The incidence of durotomy during primary lumbar fusion and its economic impact are not well described. METHODS The Nationwide Inpatient Sample was queried for all primary 1- or 2-level lumbar fusions performed in adults for lumbar spinal stenosis between 2009 and 2011; only elective cases without concurrent diagnoses of vertebral infection, fracture, or tumor were included. χ and t-tests were used as appropriate to compare categorical and continuous variables, respectively. Multivariate regression analysis was performed to identify factors independently associated with incidental durotomy, as well as total hospital charges, costs, and length of stay. RESULTS Among 17,232 cases, 802 incidental durotomies were identified (rate 4.65%). The multivariate odds of durotomy in the oldest patients (age ≥ 73) were 2.4 times greater than the odds of durotomy in the youngest patients (age ≤ 56; P < 0.0001). Durotomy was associated with increased neurological complications and longer hospital stay. Length of stay was a significant driver of cost. The multivariate odds of dural tears in teaching hospitals was significantly higher compared with nonteaching hospitals (odds ratio 1.27; 95% confidence interval, 1.06-1.52; P < 0.005). Durotomy was associated with a $10,885 increase in total hospital charges, and a $3,873 increase in estimated total costs (compared with no durotomy group with P < 0.0001). CONCLUSION Increasing age is a risk factor for durotomy in primary lumbar fusion. Durotomy is associated with neurological complications, increased length of stay, greater healthcare costs, and is more common in teaching hospitals. Length of stay is an independent driver of cost and complications. LEVEL OF EVIDENCE 3.
Cysteine Residues C489 and C1135 of NaV 1.5 Play a Critical Role in Producing Late Sodium Current through nNOS-Dependent S-Nitrosylation of NaV 1.5

Direct S-nitrosylation of cysteine residues (SNO) in the cardiac sodium channel (NaV1.5) has been shown to increase late sodium current (INa) and has been implicated as a key biophysical mechanism underlying prolongation of the action potential in Long QT Syndromes type 9 and 12. The specific SNO site(s) of NaV1.5 that regulate increased late INa have not been established and we sought to determine if specific cysteine residue(s) in NaV1.5 mediate SNO-dependent increase of arrhythmogenic late INa. Cysteine residues were identified as candidates for S-nitrosylation by similarity to consensus sequences for S-nitrosylation and individual residues in NaV1.5 were replaced with alanine using site-directed mutagenesis (C280A, C374A, C489A, C649A, C683A, C686A, C1135A, C1166A, C1171A, and C1849A). Mutant or wild-type NaV1.5 was co-expressed in transfected HEK293 cells with the neuronal nitric oxide synthase (nNOS), and the adaptor protein α1-syntrophin (SNTA1), to optimize NaV1.5 nitrosylation. Using the whole-cell patch clamp technique, INa was recorded and late INa measured between 600-700ms as a percentage of peak INa. Wild-type NaV1.5 showed 0.80±0.07% (n=99) late INa at −20 mV which was decreased by the nNOS inhibitor L-NMMA to 0.24±0.05% (n=9). Only two Cys mutants showed reduced nitrosylation-dependent late INa; C489A (0.48±0.06%, n=32, p = 0.009) and C1135A (0.45±0.07%, n=29, p = 0.007). When the nNOS inhibitor L-NMMA was added to cells expressing C489A or C1135A the level of late INa was similar to L-NMMA treated wild-type (0.28±0.14%, n=7; 0.28±0.06%, n=8, respectively). The double mutation of C489A and C1135A showed no significant nitrosylation-dependent late INa (0.26±0.04%, n=22, p = 0.0008). These data provide evidence that two specific cysteine residues (C489 and C1135) on NaV1.5 account for nitrosylation-dependent late INa.
A Novel Bandpass Filter Based on DSCSRR

Inspired by CSRR(complementary split ring resonator),a novel structure named DSCSRR(diamond shaped complementary split ring resonator) was proposed in this article to enhance the selectivity of bandpass filter.And then its left-handed characteristics were presented and its transmission characteristic was studied.In the last a bandpass filter based on DSCSRR was designed and fabricated.The agreement of simulation and measurement results indicates that the proposed filter's working frequency is 4.02 GHz,and its selectivity is 170dB/GHz,and insertion loss 15dB.The proposed filter have some advantages such as high selectivity,simple in design,and also compact in size.
Fault Analysis and Improvement Measures on Compensation Capacitor and Series Reactor

In this paper,one fault of compensation capacitor and series reactor at certain two substations of Southern power grid of Hebei is described in detail.The test analysis and equipment analysis are performed through inspection of capacitor and reactor,the cause of such fault is found and,on this basis,related improvement measures are raised,which provides reliable assurance for the safe operation of power grid.
A chironomid-based reconstruction of late-Holocene climate and environmental change for southern Pacific Costa Rica

A lake sediment profile spanning the last ~3200 years from Laguna Zoncho in the southern Pacific region of Costa Rica was analyzed for sub-fossil chironomids. Notable shifts in chironomid assemblages occurred during the late-Holocene. A distinct chironomid community, dominated by Tanypodinae such as Procladius and Labrundinia, appeared after ~550 cal. yr BP (~1400 CE). Prior to this time, the chironomid assemblage was more diverse, with taxa such as Paratanytarsus, Tanytarsus type N, and Cladotanytarsus important constituents of the chironomid community. A chironomid-based inference model for mean annual air temperature (MAAT), developed using partial least squares (PLS 2-component), was applied to sub-fossil chironomid assemblages from Laguna Zoncho to reconstruct late-Holocene thermal variability for the region. The key findings from this study are as follows: (1) chironomid-inferred MAAT at ~2740–1220 cal. yr BP (790 BCE–730 CE) was 1.2°C higher than the late-Holocene (~3200 cal. yr BP to present in th...
Calling Out Publics

The intellectual’s role generally is dialectically, oppositionally … to challenge and defeat both an imposed silence and the normalized quiet of unseen power wherever and whenever possible.—Edward ...
Edible Luminescent Probes as Sensors of Food Quality:Identification and Selection

Foods contain a plethora of aromatic molecules—natural colors, synthetic dyes, flavors, vitamins, antioxidants—that are luminescent, exhibiting prompt fluorescence or delayed phosphorescence. Although food autofluorescence has been used to assess food composition (e.g., quantification of vitamins), to detect specific contaminants (e.g., aflatoxins) or, more recently, to authenticate specific foods (e.g., luminescence fingerprinting of olive oil), much of the potential of using the optical luminescence of intrinsic molecules for sensing properties of foods is unrealized. Relatively low quantum yields, short lifetimes under conditions of interest in food testing and/or lack of comprehensive knowledge on the sensitivity of luminescence signals from native or commonly added chromophores to food quality attributes are some of the reasons that hinder their utilization.We summarize here work characterizing the photophysical properties of some edible, generally recognized as safe (GRAS) chromophores and their sensitivity to, and thus potential for sensing, various physical and chemical properties of food known to reflect or be indicative of food quality, stability, and safety. Edible luminescent molecular rotors offer a non-disruptive and highly sensitive alternative to conventional mechanical methodologies to evaluate the physical properties of foods. Flavonols that exhibit dual fluorescence and a ratiometric and concentration independent response to their solvent environment are particularly promising probes of water activity (aw). Food grade compounds with known active triplet states provide a feasible way to assess the stability of food matrices by monitoring molecular mobility and oxygen diffusion in amorphous solids.A thorough characterization and interpretation of the luminescent signals from edible chromophores can expand the repertoire of analytical techniques available to monitor quality, and even safety, of the food supply at various stages of the production and distribution chain or at point of sale. The potential of edible fluorophores as food quality sensors, their properties and limitations are discussed herein.
Painting and Politics in El pintor de su deshonra: Monarchy As a Work of Art

AbstractPainting and politics are closely allied in Calderon’s El pintor de su deshonra. Don Juan Roca is a dishonoured husband and a failed artist whose attempt to render Serafina’s divine perfection on canvas by painting her beauty provokes an ontological crisis that manifests itself in political terms as a crisis of monarchy. As the orthogonals of a comedia structured according to the principles of linear perspective, don Juan, don Alvaro and the Prince converge on Serafina, whose tragic death in the vanishing point of the play calls into question a theocratic understanding of the ideal Christian prince, thereby freeing virtue and power to relate to each other like the figures in a Renaissance canvas. Having seen the blood-drenched destruction brought about in part by his desire to possess the divine as symbolized in the figure of Serafina, the Prince then offers his hand in marriage to Porcia and takes his place in the final ‘painting’ of the play: which is that of monarchy as a work of art.
Double Integral Sliding Mode Control for Single-Stage Active FIBC-PFC in Smart Grid Applications

This work addresses a single-stage active power factor correction (PFC) scheme that is implemented using a Floating Interleaved Boost Converter (FIBC) equipped with an indirect Double Integral Sliding Mode Controller (DISMC) adopted for a single phase full bridge rectifier. Due to the high gain, high efficiency and small size components, FIBC-based approach can achieve unit power factor and voltage-regulation within a single-stage configuration in addition to optimize the system configuration. An indirect DISMC scheme is proposed to reduce the steady-state error and chattering while achieving fast transient and reasonable stability. The proposed controller can effectively improve the tracking performance of the line current. Simulations have been done by MATLAB indicate that, the proposed approach drives the output voltage to the desired DC level in the presence of external disturbances and internal parameter uncertainties, while providing close to unity power factor.
HVDC converter transformer saturation in hybrid AC/DC system caused by coupled transmission lines

Hybrid ac/dc transmission can increase the power transfer capability of long ac transmission lines. High voltage dc (HVDC) converters are needed, and the line-commutated converter (LCC) is used considering the dc fault current controllability. However, zero-sequence current can be generated due to the coupled transmission lines, and it will flow into the HVDC converters as a fundamental frequency current component on dc side (i60). The LCC will convert i60 to dc current components on the ac side, which may cause potential converter transformer saturation. This paper analyzes the influence of coupled transmission lines on i60 and the converter transformer saturation and proposes two possible solutions to avoid converter transformer saturation. The simulation results verify the effectiveness of the proposed methods.
A Linear Control Algorithm for a Flat Glass Annealing LEHR

Abstract Flue temperatures are used to regulate the glass temperature in an annealing lehr. Glass temperature control is then a process in which a number of parallel interactive control loops are encountered. When a load change occurs in the glass ribbon, flue set point changes are required to maintain a desired temperature profile for the glass ribbon. The necessary set point changes can be predicted using a simple thermal model of the system to account for the interaction. This model can then be used as a supervisory algorithm to predict the required set point changes in a real time environment.
Electrodeposited copper-graphite composites for low-CTE integrated thermal structures

Abstract Emerging high-power and high-temperature electronic modules require thick copper structures for power supply, thermal vias, heat spreaders, and also as carriers or lead frames for high-power packages. Such structures should coexist with glass and other substrates with low coefficient of thermal expansion (CTE) to meet high-temperature performance, dimensional stability, and superior device interconnection reliability with low stresses and warpage. The primary challenge with these packages arises from the CTE mismatch between the conductors and the substrates. Cu-graphite composites with glass-matched CTE are explored to address this challenge through analytical modeling of properties such as CTE, Young's modulus and thermal conductivity, finite-element-modeling predictions of glass warpage and stresses, process development to deposit copper-graphite composite films with high graphite loading of 64 vol. %, and warpage measurements using shadow moire. Results indicate that Cu-graphite composites ca...
Quantifying the relationship between social capital and economic conditions in Appalachia

Abstract Researchers and practitioners from numerous fields are increasingly aware of the limits of conventional economic development, which is development that tends to rely on external investments (e.g., in infrastructure and business recruitment) to promote economic growth in a given area. Accordingly, a host of alternative strategies that aim to build new and leverage existing local resources and networks are quickly re-populating community and economic development arenas. Among the most prominent ideas in this shift from externally-controlled to comparatively bottom-up and locally-driven development is social capital. Because of its prominence in both academic and practical development discourses, the association of social capital with economic performance receives ample attention—though empirical evidence in this area is mixed. In this article, we adopt the American Appalachian region as a case study in which to explore this relationship using tools of applied spatial analysis. Appalachia offers an intriguing case for such research, as a powerful multijurisdictional economic development agency in the region—the Appalachian Regional Commission (ARC)—has recently shifted from using predominantly conventional economic development tools to promoting efforts to build social capital in Appalachian communities. Using a combination of geovisualization, cluster analysis, and correlation analysis, this article reveals a strong and inverse association between spatial patterns of an established proxy indicator of social capital and the ARC's index of economic distress. The results offer encouragement both to the ARC and related institutions that are abandoning conventional growth-oriented practices for locally-driven development initiatives.
Psychological Ownership in Financial Decisions

Policy makers have long intuitively realized the benefit of encouraging strong psychological ownership toward social programs. For example, Franklin D. Roosevelt in the 1930s purposely designed the Social Security program to include a high feeling of ownership by workers, to generate a sense of responsibility and protection from future legislation. Recent research suggests that this sense of ownership continues to be an important part of the SSA program and is a significant predictor of when retirees claim their benefits. Strong feelings of ownership also exist for other programs (e.g., Medicare) and financial services (e.g., investments), and there are significant implications of that psychological ownership on consumers’ decisions and behavior around these programs. Policy makers and marketers may wish to consider the role of interventions that affect consumers’ psychological ownership, along with feelings of trust and fairness, for such products.
Masculine Gender Role Stress

Over the past decade there has been unprecedented interest in fostering improved health practices through lifestyle change and stress reduction. This interest is reflected in the popular literature as well as in health-related research. Whereas the burden of health-related problems was once imposed by infectious diseases, today many serious health problems result from unhealthy life styles (Baffi, Redican, Sefchick, & Impara, 1991). As interest in health research has grown, so has attention to identifying factors that make distinctive populations of individuals uniquely vulnerable to various forms of stress and illness.
Revuz Measures and Related Formulas on Energy Functional and Capacity

In the context of right Markov processes, Revuz measures of additive and multiplicative functionals are investigated. Using Revuz measures, several formulas on the energy functional and capacity are given to describe the relationships between any process and its subprocesses.
Targeting Cancer at the Intersection of Signaling and Epigenetics

While mutations resulting in the chronic activation of signaling pathways drive human cancer, the epigenetic state of a cell ultimately dictates the biological response to any given oncogenic signa...
Teaching Excel to undergraduate students: Do the platform constraints of Windows versus Mac make a difference?

The authors’ objective was to uncover the difference in competency when teaching Excel to undergraduate students on two distinct and discrete operating platforms. The authors calibrated competency ...
TEMPERATURE, WATER AVAILABILITY, AND NUTRIENT LEVELS AT VARIOUS SOIL DEPTHS-CONSEQUENCES FOR SHALLOW-ROOTED DESERT SUCCULENTS, INCLUDING NURSE PLANT EFFECTS

Implication des variations interspecifiques des profondeurs d'enracinement dans la prediction de la croissance et de la survie de Agave deserti et de Ferocactus acanthodes eventuellement associes a Hilaria rigida. Les resultats permettent d'etendre cette prediction au comportement des deux especes etudiees selon la pluviosite annuelle
High-Power Indium Phosphide Photonic Integrated Circuits

Indium phosphide (InP) is the most developed platform for photonic integrated circuits (PICs). Of interest is the advancement of this platform for applications that demand high performance, especially high output power, including free space communications and microwave photonics. In this paper, we summarize development of InP-based PIC transmitters. Two transmitter types were fabricated: one based on an offset quantum wells (OQW) platform and the other on a quantum well intermixing (QWI) platform. The OQW-based transmitter consists of a widely tunable laser, a high-speed semiconductor optical amplifier (SOA), a Mach–Zehnder modulator, and an output SOA. This transmitter demonstrates a 44-nm tuning range, >45 dB side mode suppression ratio, 14.5 dBm off -chip power, and a data rate of 7 Gbps. The second transmitter, based on QWI, utilizes an alternate epitaxial structure to achieve a lower confinement factor for higher SOA output saturation power. This QWI transmitter consists of a distributed Bragg reflector laser, a high-speed SOA, an electroabsorption modulator, and an output SOA. The measured off -chip power is 19.5 dBm, and a data rate of 20 Gbps is demonstrated. Based on the improved performance with the new epitaxial structure, a novel platform for high-power PIC transmitters integrated with low confinement and high-power SOAs is described.
Protein quality control in the secretory pathway

Protein folding is inherently error prone, especially in the endoplasmic reticulum (ER). Even with an elaborate network of molecular chaperones and protein folding facilitators, misfolding can occur quite frequently. To maintain protein homeostasis, eukaryotes have evolved a series of protein quality-control checkpoints. When secretory pathway quality-control pathways fail, stress response pathways, such as the unfolded protein response (UPR), are induced. In addition, the ER, which is the initial hub of protein biogenesis in the secretory pathway, triages misfolded proteins by delivering substrates to the proteasome or to the lysosome/vacuole through ER-associated degradation (ERAD) or ER-phagy. Some misfolded proteins escape the ER and are instead selected for Golgi quality control. These substrates are targeted for degradation after retrieval to the ER or delivery to the lysosome/vacuole. Here, we discuss how these guardian pathways function, how their activities intersect upon induction of the UPR, and how decisions are made to dispose of misfolded proteins in the secretory pathway.
The Synthesis Success Calculator: Predicting the Rapid Synthesis of DNA Fragments with Machine Learning

Abstract The synthesis and assembly of long DNA fragments has greatly accelerated synthetic biology and biotechnology research. However, long turnaround times or synthesis failures create unpredictable bottlenecks in the design-build-test-learn cycle. We developed a machine learning model, called the Synthesis Success Calculator, to predict whether a long DNA fragment can be readily synthesized with a short turnaround time. The model also identifies the sequence determinants associated with the synthesis outcome. We trained a random forest classifier using biophysical features and a compiled dataset of 1076 DNA fragment sequences to achieve high predictive performance (F1 score of 0.928 on 251 unseen sequences). Feature importance analysis revealed that repetitive DNA sequences were the most important contributor to synthesis failures. We then applied the Synthesis Success Calculator across large sequence datasets and found that 84.9% of the Escherichia coli MG1655 genome, but only 34.4% of sampled plasmids in NCBI, could be readily synthesized. Overall, the Synthesis Success Calculator can be applied on its own to prevent synthesis failures or embedded within optimization algorithms to design large genetic systems that can be rapidly synthesized and assembled.
Teaching about Restorative Justice: By Aida Y. Hass-Wisecup, and Caryn E. Saxon, Durham, NC: Carolina Academic Press, 2018, 372pp., $55, ISBN: 978-1-5310-0300-5; e-ISBN: 978-1-5310-0735-5

There are a lot of print materials to choose from when planning a new undergraduate course that will focus on, or discuss restorative justice. Both the International Institute for Restorative Pract...