Evolution and complexity of micro RNA in the human brain.

The discovery of small non-coding RNAs (sncRNAs), and the biological actions of these ribonucleotide entities in the developing, aging, and pathological human central nervous system (CNS) has opened a novel and fascinating vista into our appreciation of human brain epigenetics, the role of sncRNAs on homeostatic and pathogenic gene control, and the potential role of single stranded, 22n Ambros, 2004, 2011; Lukiw, 2007; Lukiw and Pogue, 2007; Guo et al., 2010). Indeed, a major family of sncRNAs known as micro RNAs (miRNAs) has emerged as essential and critical regulators of gene expression in diverse neurobiological pathways in both health and disease. The primary mode of action of miRNAs is to bind to target ribonucleotide sequences in messenger RNAs (mRNAs), typically in their 3′ un-translated region (3′-UTR), resulting in a highly selective repression of the readout of that mRNA's genetic information (Lukiw, 2007; Guo et al., 2010; Ambros, 2011). Pathogenically up-regulated miRNAs can be considered an epigenetic mechanism to down-regulate specific mRNAs and their expression. Up-regulated miRNA in human brain-specific neurodegenerative disorders such as Alzheimer's disease (AD) may help explain the large number of essential brain gene messages observed to be significantly down-regulated in AD affected anatomical regions, such as in the temporal lobe neocortex (Brodmann area 22) and hippocampus, or in primary human brain cell cultures that are chronically subjected to AD-relevant stressors (Loring et al., 2001; Colangelo et al., 2002; Lukiw, 2004, 2012; Lukiw and Pogue, 2007; Sethi and Lukiw, 2009; Ginsberg et al., 2012; Lukiw and Alexandrov, 2012; Lukiw et al., 2012; Wang et al., 2012). While the total number of human miRNAs identified to date in all tissues numbers around 2000, the number of abundant human brain miRNAs is surprisingly far less; for example only about one-thirtieth of all so far identified human miRNAs are abundantly present in the human brain association neocortex (Table ​(Table1).1). Further, mathematical, bioinformatical, and nucleotide sequence analysis indicate that a 22n Lukiw and Alexandrov, 2012). Table ​Table11 lists the 61 most abundant miRNAs found in a critically controlled pool of short post-mortem interval (PMI; <3h total N&=&21) human association neocortex (Brodmann area A22) indicating that only about 61 of 1013, or less than one in one hundred trillion of all potential miRNA species possible have been selected to play some role in brain-essential regulatory functions as a brain abundant miRNA. An additional interesting fact is that miRNAs are highly developmental stage-, tissue- and cell-specific, even in adjacent cell types, and mean abundance of these individual species may fluctuate as do the levels of transcription factors which regulate pre-miRNA transcription (Burmistrova et al., 2007; Lukiw and Pogue, 2007; Guo et al., 2010; Lukiw and Alexandrov, 2012). The stability, and hence persistence of action, of sncRNAs, and miRNA appear to follow the adenine-uracil (AU) element (ARE) rich rules as for mRNA stability, and the half-life of several brain abundant miRNAs has been observed to be surprisingly restricted (Sethi and Lukiw, 2009; Wang et al., 2012). For example, human brain miRNA half-lives have been estimated to range from about 30&min (miRNA-9) to about 3&h (miRNA-125b), so overall the generation of a new miRNA, its longevity and interdiction of translational processes may be relatively rapid neurogenetic process (Sethi and Lukiw, 2009). However, other biophysical and neurogenetic parameters such as miRNA secondary and tertiary structure, miRNA binding proteins, and other factors may significantly extend miRNA viability and action. Table 1 Most abundant miRNAs in the human brain temporal lobe association neocortex; ranked by miRNA numerical designation; based on pooled data from N = 21 short post-mortem interval (PMI) controls; this group included 9 males and 12 females, ... Another poorly understood layer of complexity in miRNA signaling is that several miRNAs may regulate a single mRNA in a neurogenetic control process known as miRNA convergence, and conversely, a single miRNA may interact with multiple mRNAs in a regulatory process known as miRNA divergence (Lukiw and Alexandrov, 2012). Multiple functioning, and sometimes overlapping, miRNA binding sites have been found, for example, in the 232n Lukiw et al., 2012). Indeed, overlapping of miRNA binding sites in the 3′-UTR of the same mRNA may be an evolutionary strategy to regulate specific gene expression via a common genetic signal in multiple and related cell types (Lukiw and Alexandrov, 2012; Lukiw et al., 2012). Anti-miRNA (antagomir, AM) approaches to these multiple miRNA binding sites in mRNA 3′-UTRs are currently redirecting our therapeutic perspectives to potentially yield novel and highly efficacious treatment strategies for brain diseases that have not yet been considered (Lukiw et al., 2008, 2012; Cui et al., 2010; Wang et al., 2012). It will be particularly interesting to analyze global miRNA–mRNA targeting and control patterns in the brain and CNS to further unravel this fascinating, evolutionarily selective regulatory networking. How individual sncRNA, miRNA, and mRNA complexity together become progressively altered in aging and disease, and their selective quenching using novel AM strategies should be of high therapeutic value in the homeostatic stabilization of miRNA complexity, and in the treatment of common, age-related degenerative disorders, both inside and outside of the central nervous system.