cssi/SciSciGPT-SciSciNet huggingface dataset:
Geodetic Data Assimilation for Evaluating Volcanic Unrest

Ensemble based data assimilation approaches, such as the Ensemble Kalman Filter (EnKF), have been widely and successfully implemented to combine observations with dynamic forecast models. In this study the EnKF is adapted to assimilate ground deformation observations from interferometric synthetic-aperture radar (InSAR) and GPS into thermomechanical finite element models (FEM) to evaluate volcanic unrest. Two eruption hindcasts are investigated: the 2008 eruption of Okmok volcano, Alaska and the 2018 eruption of Sierra Negra volcano, Galapagos, Ecuador. At Okmok, EnKF forecasts tensile failure and the lateral movement of the magma from a central pressure source in the lead up to its 2008 eruption indicating potential for diking. Alternatively, at Sierra Negra, the EnKF forecasts significant shear failure coincident with a Mw 5.4 earthquake that preceded the 2018 eruption. These successful hindcasts highlight the flexibility and potential of the volcano EnKF approach for near real time monitoring and hazard assessment at active volcanoes worldwide.
Predicting hydration and moisturizer ingredient effects on mechanical behavior of human stratum corneum

Abstract Hydration of the outermost layer of human skin, the stratum corneum (SC), affects barrier function, physical appearance, and sensory perception of skin. Water loss, ubiquitously associated with dry skin conditions, results in the development of mechanical tensile stresses that lead to the perception of skin tightness and provide a driving force for damage in the form of chapping or cracking. Restorative moisturizing formulations contain molecular components that counteract the effects of water loss. However, the quantitative connection between water loss and SC mechanical behavior together with a predictive model has remained elusive. We develop a diffusional-based mechanics model that accurately predicts the SC mechanical behavior during hydration or dehydration over a wide range of ambient humidity conditions and presence of moisturizing ingredients. The model was validated with published studies including SC water diffusion, quantitative Raman spectroscopy, and mechanical property and stress characterization. The roles of mobile and partially bound water states are included together with one humectant and three cosmetic emollient molecules commonly used in moisturizing formulations. The model accurately predicts the effects of moisture content and moisturizer ingredients on SC mechanical behavior and provides a quantitative predictive capability that can be employed in the design of efficacious treatments.
Book Review of: Stephen A. Smith, Rights, Wrongs, and Injustices: The Structure of Remedial Law (Oxford University Press, 2019)

This paper is a draft review of Stephen Smith’s recent book -- Rights, Wrongs, and Injustices: The Structure of Remedial Law (Oxford University Press, 2019). The book offers a groundbreaking and deeply insightful theory of the remedies in private law. On Smith’s account, remedies are judicial rulings, and they are issued because they provide people with new reasons for action. This review will focus on a jurisprudential puzzle that lies at the center of the book. Rights, Wrongs, and Injustices provides an original account of the authority in court orders. I will suggest that the book is right that the authority in court orders is distinctive, but wrong in its analysis of what grounds that authority. Considering this question, however, sheds significant new light on the law of remedies and on private law as a whole.
Utility of Exercise Stress Testing in Pediatric Patients with T-Wave Inversions

T-wave inversions (TWI) in inferolateral electrocardiogram (ECG) leads (II, III, aVF, V5–V6) are often suggestive of cardiac pathology in adult patients. They are a common reason of additional testing in pediatric patients. The goal of the study is to determine correlation of exercise stress test T-wave response with diagnosis of cardiac pathology. This is a retrospective review of pediatric patients < 21 years with the finding of baseline TWI in inferior (II, III, avF) and/or lateral (V5–V6) leads on 12-lead ECG. All patients underwent treadmill exercise stress test (EST) and an echocardiogram within 1 year of each other. Demographics, baseline ECG findings, echocardiogram results, and EST data were recorded. T-wave reversion was considered complete if T waves demonstrated normalization during exercise, partial if there was minimal improvement, and no response if there was no change or worsening of inversions. In our cohort of 72 patients with a mean age 14.6 (± 2.9) years and 61% males, 59 (82%) had a structurally normal heart. Thirteen patients had evidence of structural or functional heart disease. Of the 59 patients, 83% had either complete or partial T-wave response. Among the 13 patients with heart disease, two patients had genetic testing consistent with risk for hypertrophic cardiomyopathy and had complete and partial response on EST. Exercise stress testing for TWI in pediatric patients has low sensitivity and specificity for the diagnosis of cardiac disease and routine use in this patient population may not be indicated.
A Report on Michigan Laws Regulating Burials, Cremations, and Cemeteries

The Michigan Constitution grants the Legislature the power to pass laws to promote and protect public health. While the Michigan Constitution does not specifically mention burials, handling dead bodies, or any other subject related to death and dying, regulating these areas helps promote and protect public health. Thus, the Michigan Legislature has used this authority to promulgate numerous statutes regulating this area. This report summarizes these statutes in an effort to provide a broad-based overview of this area of law. This report was originally drafted to assist the Michigan Law Revision Commission in discovering defects and anachronisms in the law that should be addressed by the Michigan Legislature. However, this report is particularly useful to any practitioner or academic looking for a discussion of the law in these areas.
Analytical methods for a national study of chemical residues in fish: I: Polychlorinated dibenzo-p-dioxins/dibenzofurans

Abstract Analytical Methods were developed to determine the concentration of PCDD/PCDF in fish tissue for a national survey conducted by EPA. The method was designed to achieve a relatively low cost per sample while maintaining adequate quality control. It utilizes a Soxhlet extraction, acid-celite, florisil and carbon-silica gel chromatography, and HRGC/HRMS analysis. The minimum level of detection for 2,3,7,8-TCDD is approximately 1 pg/g. Rigorous quality assurance/quality control criteria have been developed to assure the validity of the data.
Toward a Stricter Originality Standard for Copyright Law

In order to be copyrighted, a work of art must be 'original." Critics have persuasively argued that copyright law, at various phases in its evolution, has defined originality by applying a Romantic conception of authorship, according to which the author creates out of a wholly personal, original self. But, in contrast to the idealized, Romantic work, an actual work need only exhibit an "extremely low" level of originality in order to merit copyright protection. This Note attempts to resolve this apparent tension between theory and practice, arguing that the Romantic conception of authorship underlies the law's low originality standard. Further, the Note argues that the modern understanding of authorship, which recognize's that the outside world shapes the author's consciousness, furnishes a more appropriate model for originality jurisprudence. Accordingly, the Note concludes, a stricter originality standard is needed, which would serve to reinvigorate the public domain while protecting truly original works.
Concrete Semantics for Pushdown Analysis: The Essence of Summarization

Pushdown analysis is better than finite-state analysis in pre- cision and performance. Why then have we not seen total widespread adoption of these techniques? For one, the known techniques are technically burdened and difficult to understand or extend. Control structure of the programming language gets pulled into the model of computation, which makes extensions to non-pushdown control structures, such as call/cc or shift and reset, non-trivial. We show a derivational approach to abstract interpretation that yields transparently sound static analyses that can precisely match calls and returns when applied to well-known abstract machines. We show that adding memoization and segmenting the continuation into bounded pieces leads to machines that abstract to static analyses for context-free reachability by simply bounding the stores. This technique allows us to derive existing, more technically involved analyses, and a novel pushdown analysis for delimited, composable control.
Diffuse endobronchial polyposis following a titanium tetrachloride inhalation injury.

We report a case of diffuse endobronchial polyps contributing to continued respiratory failure in a patient 5 wk after titanium tetrachloride inhalation injury. After the institution of corticosteroid therapy, the total number and size of remaining polyps were markedly reduced at which time the subject was successfully weaned from the ventilator. Follow-up bronchoscopy at 2 months, 5 months, and 1 yr postinjury revealed gradual but complete resolution of all polyps accompanied by improvement in the patient’s obstructive airways disease. This delayed complication of inhalation injury has been previously reported as a complication of thermal respiratory injury, and may be an important contributing factor in prolonged or late respiratory failure in burn patients.
Impact of vitamin D deficiency upon clinical presentation in nasal polyposis

Background The objective of this work was to determine if specific chronic rhinosinusitis with nasal polyps (CRSwNP) populations are at risk for vitamin D3 (VD3) deficiency and if VD3 levels correlate with radiographic measures of disease severity or eosinophilia. Methods This study was a retrospective review of an academic rhinology practice. CRSwNP patients who had VD3 levels and CT scan within 6 months of each other were included. CT scans were graded using Lund-Mackay scoring (LMS) and peripheral eosinophil counts were measured. Demographic data including race, gender, age, body mass index, atopic status, and presence of asthma were collected. CRSwNP was subdivided into allergic fungal rhinosinusitis (AFRS), aspirin-exacerbated respiratory disease (AERD), and other CRSwNP. Multivariate analysis was performed to examine correlations and control for confounding factors. Results Insufficient VD3 levels were found in 55% of all CRSwNP patients. VD3 correlated with African American race because nearly 80% of all African Americans had insufficient VD3 levels. Lower VD3 levels also correlated with more severe mucosal disease on CT scans as measured by LMS. There was no correlation between VD3 levels and age, gender, body mass index, atopy, asthma, or CRSwNP subtype. Conclusion VD3 insufficiency/deficiency is common in CRSwNP patients, especially those of African American race. Lower levels of VD3 are associated with worse LMS on CT. The role of VD3 in CRSwNP warrants further investigation.
Streptomycin-induced, third-position misreading of the genetic code.

Streptomycin was used to increase the frequency of errors in protein synthesis in vivo. In the system under study two misreading errors were observed. Both involved the erroneous insertion of lysine at asparagine codons, because of misreading of a pyrimidine as a purine at the 3′ position of the codon. Streptomycin increased the errors at the two codons AAU and AAC to the same extent, thereby maintaining the error ratio found for basal level mistranslation.
Mucinous breast carcinomas lack PIK3CA and AKT1 mutations

Activating point mutations in the phosphatidylinositol-3-kinase catalytic subunit (PIK3CA) are among the most common molecular defects in invasive breast cancer. Point mutations in the downstream kinase AKT1 are seen in a minority of carcinomas. These mutations are found preferentially in estrogen receptor-positive and Her2-positive breast carcinomas; however, special morphologic types of breast cancer have not been well studied. Twenty-nine cases of pure invasive mucinous carcinoma and 9 cases of ductal carcinoma with mucinous differentiation were screened for a panel of point mutations (>321 mutations in 30 genes) using a multiplex polymerase chain reaction panel with mass spectroscopy readout. In addition, associated ductal carcinoma in situ, hyperplasia, or columnar cell lesions were separately tested where available (25 lesions). In 3 invasive cases and 15 ductal carcinoma in situ/proliferative lesions, PIK3CA hotspot mutations were, instead, tested by direct sequencing. No point mutations were identified in invasive mucinous breast carcinoma. This contrasts with the 35% frequency of PIK3CA mutations in a comparative group of invasive ductal carcinomas of no special type. Interestingly, PIK3CA hotspot point mutations were identified in associated ductal carcinoma in situ (3/14) and hyperplasia (atypical ductal hyperplasia [2/3], usual ductal hyperplasia [2/3], columnar cell change [1/5]), suggesting that PIK3CA mutations may play a role in breast epithelial proliferation. This series represents the largest study, to date, of PIK3CA genotyping in mucinous carcinoma and supports the unique pathogenetics of invasive mucinous breast carcinoma.
When it is hard to get to with genetics--planar cell polarity under a chemical scalpel.

Planar cell polarity (PCP) has been under genetic dissection for decades. More and more fundamental developmental processes have been found relying on PCP signaling. However, mechanisms of how PCP signaling generates asymmetry is still unknown. A recent paper in Chemistry & Biology (Sundberg et al., 2011) represents the efforts to decipher the intracellular code of polarity signaling.
The Fine Structure of Colpoda maupasi with Special Emphasis on Food Vacuoles

SYNOPSIS. An electron microscope study of Colpoda maupasi Enriques, isolated from the intestine of the blue-tongued skink Tiliqua nigrolutea, showed that the fine structure of this ciliate is similar in all respects to that of free-living ciliates. The correspondence applies particularly to the structure, distribution and number of mitochondria. This organelle has a rich intramitochondrial structure in the form of microvilli; it is found close to the periphery, near the nuclear apparatus and in other parts of the cytoplasm. It was concluded that the association between Colpoda maupasi and Tiliqua nigrolutea was probably accidental and limited to the cyst stage. Thus electron microscopy confirmed a conclusion arrived at by light microscopy. The presence of numerous food vacuoles made it possible to study stages of digestion within this organelle. Four major types of food vacuole were distinguished. Type 1 food vacuoles are characterized by their large size, the presence of intact bacteria and abundance of water. In type 2 the food vacuole is deprived of water, the bacteria are pressed together and the nuclei have lost their structure. Type 3 food vacuoles contain only bacterial ghosts, cytoplasmic and nuclear material having been digested. Food vacuoles of this type are found only occasionally, suggesting their short duration. It is of interest that during this transient stage the bulk of digestion takes place. In type 4 nothing reminiscent of bacteria is found; there are only myelin figures and vesicles of different sizes. Evaginations and invagnations of the vacuolar membrane and vesicles of different size and structure inside and outside the food vacuoles of types 1, 3 and 4 suggest that extensive communication exists between the cytoplasm and the food vacuole. It seems likely that enzymes are delivered to the food vacuole and digested materials are released from the food vacuole to the cytoplasm.
Fano-noise-limited CCDs

Recent developments of scientific CCDs have produced sensors that achieve ultra low read noise performance (less than 2 electrons rms) and near perfect charge transfer efficiency (0.9999996) without the addition of a fat-zero. This progress has now made it possible to achieve Fano-noise-limited performance in the soft X-ray where the detector's energy resolution is primarily limited by the statistical variation in the charge generated by the interacting X-ray photon. In this paper, Fano-noise-limited test data is presented for two different CCD types and a CCD derived estimate of the Fano factor is determined. By evaluating ultra low-modulation images (less than 1 electron peak-to-peak) it is shown that the CCD's global CTE is now superior to its read noise floor. To capitalize on this capability CCD manufacturers are now focusing their attention on reducing the noise floor below the 1 electron level thereby matching the sensor's CTE performance. This improvement, if accomplished, will push Fano-noise-limited performance for the CCD into the extreme ultra-violet.
Crosstalk between the EGFR and LIN-12/Notch pathways in C. elegans vulval development.

The Caenorhabditis elegans vulva is an important paradigm for cell-cell interactions in animal development. The fates of six vulval precursor cells are patterned through the action of the epidermal growth factor receptor–mitogen-activated protein kinase (EGFR-MAPK) inductive signaling pathway, which specifies the 1° fate, and the LIN-12/Notch lateral signaling pathway, which specifies the 2° fate. Here, we provide evidence that the inductive signal is spatially graded and initially activates the EGFR-MAPK pathway in the prospective 2° cells. Subsequently, this effect is counteracted by the expression of multiple new negative regulators of the EGFR-MAPK pathway, under direct transcriptional control of the LIN-12–mediated lateral signal.
Old Occitan as a Lyric Language: The Insertions from Occitan in Three Thirteenth-Century French Romances

Sur les sources occitanes de l'ancien francais :pratique et declin d'insertions lyriques en vieil-occitan dans le Roman de la rose ou de Guillaume de Dole,le Roman de la Violette et La Court de Paradis.
Contextual stimuli and memory retrieval in pigeons

Pigeons were given free-operant successive discrimination training in which, on alternate days, two different interdimensional problems were employed (color positive and line angle negative; a different color negative and a different line angle positive). Between days, these problems could be construed as intradimensional ones. For one group, training was conducted on each problem in the presence of the same ambient (contextual) stimuli, while for a second group each problem was trained in a different context. For a third group, these two contexts were randomly related to the problems. Postdiscrimination stimulus generalization gradients showed that peak shifts were obtained in both the same context and the random context groups, but no peak shifts were found for the group which learned each problem in a different context. Results were consistent with Spear’s (1973) treatment of animal memory which attributes retention test performance to the operation of a “context-elicited” retrieval process. An alternative uniprocess conditioning account of these and similar data was also discussed.
Recovery of isoflavones from red clover flowers by a membrane-based process ☆

Abstract Isoflavones in red clover flowers were extracted and recovered using a new process that mainly comprised of ethanol extraction, membrane processing, micelle formation and drying. To obtain maximum isoflavone extraction an ethanol concentration between 40% and 50% in water was found to be optimal. The extracted isoflavones were processed by ultrafiltration for preliminary purification, and then concentrated by reverse osmosis. As ethanol was removed by evaporation, micelles were formed in the reverse osmosis retentate, which was dried to yield an isoflavone-enriched product. This product contained about 9% isoflavone highlighting its potential use as a direct nutraceutical supplement. Industrial relevance Isoflavones present in agricultural biomass are utilized as source of functional food supplements. Extraction and refining of isoflavones involves several steps and the process is very energy intensive. This work reports an interesting approach for extraction and refining of isoflavones by developing a new process, which is energy efficient and gives a final product, which contains sufficiently high amounts of isoflavones for consumer applications.
Biology and Control of the Leatherleaf Slug Leidyula floridana (Mollusca: Gastropoda: Veronicellidae)

Abstract Leidyula floridana (Leidy, 1851) (Mollusca: Gastropoda: Veronicellidae), also known as Florida leatherleaf slug, has long been known to be a pest of ornamental plants in southern Florida, and of food crops in the Caribbean region. Its geographic range is expanding, and it also has become an intermediate host for the nematode Angiostrongylus cantonensis (Chen, 1935) (Strongylida: Metastrongylidae), which can cause meningoencephalitis in humans and other primates if ingested. Thus, it is increasingly important that we understand its biology and management. We determined that about 50% survived more than 18 mo under laboratory conditions. It attained a maximum mean weight of about 13.4 g after 18 mo, but individuals as large as 28.5 g were occasionally obtained in our cultures. It burrowed in the soil or sought shelter during the daylight hours. Slugs began egg production when 3–7 g in weight, and deposited clusters of about 45 oval eggs in or atop the soil. Eggs measured about 7.4 mm in length and ...
Local Heat Transfer and Flow Distributions for Impinging Jet Arrays of Dense and Sparse Extent

Full-surface heat transfer coefficient distribution measurements have been made using a liquid crystal thermography technique for several cases of normally impinging jet arrays onto a flat, smooth surface within a region bounded on three sides. While the impingement target plate remains of a fixed size, the impingement jet array has been changed to cover a wide range of conditions, extending beyond the currently available literature data. Axial and lateral jet spacing values of x/D and y/D of 3, 6, and 9 have been used, all with square orientation and in-line jets. The jet plate-to-target surface distance z/D has been varied from 1.25 to 5.5. Jet Reynolds numbers ranged from 14,000 to 65,000. In the sparse array limiting case, the number of jet rows is four in the axial direction and three in the lateral direction. For the dense array limiting case, the number of jet rows is 26 in the axial direction and 20 in the lateral direction. Using both heat transfer and pressure distribution measurements, results are compared to the existing correlation of Florschuetz et al. [1], showing excellent agreement in regions of common parameters. In regions not previously reported in the literature, the present study extends the streamwsie row-averaged heat transfer coefficient correlation of [1] with a modified correlation for design use.Copyright © 2002 by ASME
Determination of Surface Heating Distribution to Establish Targeted Thermal Environments in Industry Hydrothermal Autoclaves

This article presents a method for the determination of the heat flux distributions to establish the desired growth environments in autoclave reactors. The algorithm consists of three submodels including the inverse natural convection of the fluid, the inverse heat conduction in the metal wall, and the heat conduction in the insulation layer. The technique is employed to obtain the heat flux distributions that establish the same growth environment as the one predicted by the two-dimensional isothermal wall model. Based on the results, practical heater designs are proposed to improve the growth environments in such industry growth vessels.
Metagenome Sequencing of Prokaryotic Microbiota Collected from Byron Glacier, Alaska

Cold environments, such as glaciers, are large reservoirs of microbial life. The present study employed 16S rRNA gene amplicon metagenomic sequencing to survey the prokaryotic microbiota on Alaskan glacial ice, revealing a rich and diverse microbial community of some 2,500 species of bacteria and archaea.
Evaluating security requirements in a general-purpose processor by combining assertion checkers with code coverage

The problem of malicious inclusions in hardware is an emerging threat, and detecting them is a difficult challenge. In this research, we enhance an existing method for creating assertion-based dynamic checkers, and demonstrate how behavioral security requirements can be derived from a processor's architectural specification, then converted into security checkers that are part of the processor's design. The novel contributions of this research are: — We demonstrate the method using a set of assertions, derived from the architectural specification, on a full-scale open-source general-purpose processor design, called OpenRISC. Previous work used only a single assertion on a toy processor design. — We demonstrate the use of our checker-generator tool, called psl2hdl, which was created for this research. — We illustrate how the method can be used in concert with code coverage techniques, to either detect malicious inclusions or greatly narrow the search for malicious inclusions that use rare-event triggers.
Air-breathing laminar flow-based microfluidic fuel cell.

This communication reports the design and characterization of an air-breathing laminar flow-based microfluidic fuel cell (LFFC). The performance of previous LFFC designs was cathode-limited due to the poor solubility and slow transport of oxygen in aqueous media. Introduction of an air-breathing gas diffusion electrode as the cathode addresses these mass transfer issues. With this design change, the cathode is exposed to a higher oxygen concentration, and more importantly, the rate of oxygen replenishment in the depletion boundary layer on the cathode is greatly enhanced as a result of the 4 orders of magnitude higher diffusion coefficient of oxygen in air as opposed to that in aqueous media. The power densities of the present air-breathing LFFCs are 5 times higher (26 mW/cm2) than those for LFFCs operated using formic acid solutions as the fuel stream and an oxygen-saturated aqueous stream at the cathode (∼5 mW/cm2). With the performance-limiting issues at the cathode mitigated, these air-breathing LFFCs...
Partitioning behavior of chlorine and fluorine in the system apatite-silicate melt-fluid

The partitioning behavior of Cl among apatite, mafic silicate melt, and aqueous fluid and of F between apatite and melt have been determined in experiments conducted at 1066 to 1150 °C and 199–205 MPa. The value of DClapatite/melt (wt. fraction of Cl in apatite/Cl in melt) ≈0.8 for silicate melt containing less than ∼3.8 wt.% Cl. At higher melt Cl contents, small increases in melt Cl concentration are accompanied by large increases in apatite Cl concentration, forcing DClapatite/melt to increase as well. Melt containing less than 3.8% Cl coexists with water-rich vapor; that containing more Cl coexists with saline fluid, the salinity of which increases rapidly with small increases in melt Cl content, analogous to the dependency of apatite composition on melt Cl content. This behavior is due to the fact that the solubility of Cl in silicate melt depends strongly on the composition of the melt, particularly its Mg, Ca, Fe, and Si contents. Once the melt becomes “saturated” in Cl, additional Cl must be accommodated by coexisting fluid, apatite, or other phases rather than the melt itself. Because Cl solubility depends on composition, the Cl concentration at which DClapatite/melt and DClfluid/melt begin to increase also depends on composition. The experiments reveal that DFapatite/melt ≈3.4. In contrast to Cl, the concentration of F in silicate melt is only weakly dependent on composition (mainly on melt Ca contents), so DFapatite/melt is constant for a wide range of composition. The experimental data demonstrate that the fluids present in the waning stages of the solidification of the Stillwater and Bushveld complexes were highly saline. The Cl-rich apatite in these bodies crystallized from interstitial melt with high Cl/(F + OH) ratio. The latter was generated by the combined processes of fractional crystallization and dehydration by its reaction with the relatively large mass of initially anhydrous pyroxene through which it percolated.
Modeling of phase separation in PEG–salt aqueous two-phase systems

A molecular model based on the integral equation theory of statistical thermodynamics is used to study phase separation in PEG–salt aqueous two-phase systems. PEG molecules are modeled as hard spheres that attract each other through a temperaturedependent Yukawa potential, which mimics the effect of PEG–water hydrogen bonding on the attraction between PEG molecules. The salt ions are modeled as charged hard spheres interacting through a Coulombic potential. Excess thermodynamic properties due to Coulombic and Yukawa interactions are calculated by analytical solutions to the Ornstein–Zernike equation for the mean spherical approximation closure. Yukawa parameters for PEG–PEG interactions are determined by fitting the theoretical phase diagram for a pure Yukawa fluid to the experimental phase diagram for a PEG–water mixture. The model predicts experimentally observed trends: increasing the temperature increases the slope and length of the tie lines; increasing the PEG molecular weight increases the miscibility gap; and increasing the anion charge lowers the salt concentration at which phase separation occurs. Theoretical results allow us to infer the relative importance of ion–PEG interactions, ion–solvent interactions, and the interpenetrable nature of PEG molecules on the phase separation in PEG–salt aqueous two-phase systems.
Antibiotic Pharmacokinetics Following Fluid Resuscitation From Traumatic Shock

Objective: To describe the pharmacokinetic profile of aztreonam and vancomycin hydrochloride in a clinically relevant experimental model of hemorrhagic shock and trauma. Methods: Ten mongrel pigs (mean±SD weight, 26.7±6.4 kg) were anesthetized with fentanyl citrate and ventilated, and an indwelling catheter was placed in the jugular vein. On day 3, all pigs were subjected to fentanyl administration, ventilation, soft-tissue injury, and an arterial hemorrhage (mean±SD, 40%±8%). After a 1-hour shock period, baseline hemodynamics were restored by reinfusing shed blood plus twice the shed volume as lactated Ringer's solution. Aztreonam and vancomycin were infused on day 1, after resuscitation on day 3, and on days 4 and 8. Serial plasma samples were collected for 6 hours after treatment, and differences were compared with analysis of variance. Results: Aztreonam clearance initially decreased with trauma, but subsequently increased by 48% ( P P =.05, baseline value vs that on day 8). Vancomycin clearance was increased between 25% and 52% ( P P =.009), but it subsequently decreased by 29% ( P Conclusions: For at least 5 days after trauma, clearance and steady-state volume of aztreonam and vancomycin are altered. These changes suggest that the interval and magnitude of dosing should be adjusted, relative to the standard recommended dosages of each antibiotic, to maximize their efficacy. Similar studies should be done for other antibiotics. (Arch Surg. 1995;130:1321-1329)
Effects of haloperidol and SCH 23390 on acoustic startle in animals depleted of dopamine as neonates : Implications for neuropsychiatric syndromes

Animals depleted of dopamine (DA) in the neonatal period and tested in adulthood exhibit some similarities to patients with schizophrenia, including increased sensitivity to DA agonists, altered sensitivity to DA receptor antagonists, and abnormalities of the acoustic startle response (ASR). In this study, we examined the contributions of D1-like and D2-like DA receptors to ASR measures in animals depleted of DA as neonates. Male rat pups received intracerebroventricular injections of 6-hydroxydopamine (DA depleted) or its vehicle (controls) at 3 days of age. Animals underwent startle testing ad adults (60–75 days of age) after administration of DA antagonists (haloperidol: 0.1 or 0.3 mg/kg, SCH 23390: 0.01 or 0.05 mg/kg) with and without DA agonist administration (apomorphine 0.5 mg/kg). ASR amplitude and prepulse inhibition (PPI: percentage decrease in startle amplitude due to a low intensity prepulse) were measured. DA depleted animals showed increased ASR amplitude and reduced PPI compared to controls. Administration of D1-like or D2-like DA antagonists significantly reduced overall ASR and increased PPI in both control and DA depleted animals, with DA depleted animals showing a relatively greater sensitivity to the D1-like antagonist SCH 23390. Findings are discussed in terms of the role of residual DA in mediating ASR phenomena in depleted animals, differences between D1/D2 DA receptor mediation of ASR compared to other behaviors in DA depleted animals, and potential implications for neuropsychiatric syndromes such as schizophrenia.
Induction of placental alkaline phosphatase in choriocarcinoma cells by 5-bromo-2'-deoxyuridine.

Growth of choriocarcinoma cells in the presence of 5-bromo-2′-deoxyuridine (BrdUrd) results in a 30- to 40-fold increase in alkaline phosphatase activity. The effect of BrdUrd is specific for phosphatase with an alkaline pH optimum. The induction by BrdUrd is probably not due to the production of an altered enzyme, since the induced enzyme resembles the basal enzyme in thermal denaturation and kinetic properties. Enzyme induction can be prevented by thymidine but not by deoxycytidine or deoxyuridine. The induction of alkaline phosphatase appears to require incorporation of the BrdUrd into cellular DNA. The presence of BrdUrd in the growth medium is not necessary for alkaline phosphatase induction in proliferating cells containing: BrdUrd-substituted genomes. However, enzyme induction and maintenance of the induced levels of alkaline phosphatase in nonproliferating cells containing BrdUrd-substituted DNA requires the presence of the analogues in the medium. The induction of alkaline phosphatase by BrdUrd in probably an indirect process.
A Subset of Cutaneous and Soft Tissue Mixed Tumors Are Genetically Linked to Their Salivary Gland Counterpart

Neoplasms morphologically similar to mixed tumors and myoepitheliomas of the salivary glands, under the broad concept of myoepithelial neoplasia, have recently been defined in the skin and soft tissue; however, to date, no data have supported a shared genetic background with their salivary gland counterpart. From a large body of research, it has been well established that rearrangement of pleomorphic adenoma gene 1 (PLAG1) leads to aberrant expression of its protein and is pathogenically relevant in the development of salivary mixed tumors. On the other hand, in soft tissue lesions, compelling evidence suggests that EWSR1 is involved in a significant subset. To examine the hypothesis that there is a genetic link between these histologically similar tumors at different sites, we randomly selected 20 benign myoepitheliomas/mixed tumors of skin and soft tissue (10 cases each). Nineteen cases could be immunostained for PLAG1, of which 11 cases showed distinct nuclear staining with moderate or strong intensity in a significant number of cells. Interphase fluorescence in situ hybridization for PLAG1 was successfully performed in 11 cases (seven in skin and four in soft tissue) and was positive for gene rearrangement in eight cases (five in skin and three in soft tissue). All PLAG1-rearranged tumors, except one, had clear-cut ductal structures and were immunoreactive for PLAG1. In our series, tumors with PLAG1 alteration shared a common morphologic phenotype characterized by prominent tubuloductal differentiation, suggesting that myoepithelial neoplasms with genuine salivary gland-like morphology, so-called soft tissue/cutaneous mixed tumors, are genetically related to their salivary gland counterpart.
Optimized algorithm for adaptive histogram equalization

Adaptive histogram equalization (AHE) is a useful technique for expanding local contrast in medical images. The method is based on histogram equalization of each pixel based on a local NXN image region. If the number of pixels in the NXN local region is equal to the number of grayshades in the image, the equalized histogram can be directly constructed from the distribution histogram. This optimization may permit AHE to be performed on standard medical image display workstations.
Toxicological properties of carrageenan

Food-grade carrageenan is a safe natural product prepared from seaweed. Its addition to food imparts many desirable characteristics which have allowed it to be used continuously for centuries. The long safe history of this natural food additive is confirmed by negative results in subchronic and chronic feeding studies in many animal species, mutagenicity studies and reproductive toxicity studies.
Diagnosis and conservative management of degenerative lumbar spondylolisthesis

Degenerative spondylolisthesis (DS) is a disorder that causes the slip of one vertebral body over the one below due to degenerative changes in the spine. Lumbar DS is a major cause of spinal canal stenosis and is often related to low back and leg pain. We reviewed the symptoms, prognosis and conservative treatments for symptoms associated with DS. PubMed and MEDLINE databases (1950–2007) were searched for the key words “spondylolisthesis”, “pseudospondylolisthesis”, “degenerative spondylolisthesis”, “spinal stenosis”, “lumbar spine”, “antherolisthesis”, “posterolisthesis”, “low back pain”, and “lumbar instability”. All relevant articles in English were reviewed. Pertinent secondary references were also retrieved. The prognosis of patients with DS is favorable, however, those who suffer from neurological symptoms such as intermittent claudication or vesicorectal disorder, will most probably experience neurological deterioration if they are not operated upon. Nonoperative treatment should be the initial course of action in most cases of DS, with or without neurologic symptoms. Treatment options include use of analgesics and NSAIDs to control pain; epidural steroid injections, and physical methods such as bracing and flexion strengthening exercises. An up-to-date knowledge on diagnosis and prevention of lumbar DS can assist in determination of future research goals. Additional studies are required to establish treatment protocols for the conservative treatment of DS.
The Use of High Magnesium Limestone in the Diet of the Laying Hen 2. Calcium and Magnesium Availability

Abstract INTRODUCTION THOUGH the majority of early reports (Hart et al., 1923; Kennard, 1925; and Halpin and Hayes, 1925) all indicated the detrimental effects of dolomitic limestone, few reports attempted to explain why they occurred. In general most experimenters agreed with the theory that magnesium exhibited some type of antagonistic effect toward calcium and that limestone should not be used as a calcium supplement unless it was free of magnesium, Alder (1927). Tully and Franke (1934) attempted to explain this antagonistic effect with the following theory. They postulated that the magnesium content of dolomitic limestone induced a cathartic action through the retention of water in the intestinal tract, and that this resulted in the rapid movement of the calcium ions through the intestinal tract. The net result they felt would be an inability to absorb enough calcium for normal shell strength. The majority of work with dolomitic limestone concerning calcium availability…
Scaling and modeling of three-dimensional, pressure-driven turbulentboundary layers

Extension au cas tridimensionnel de l'analyse asymptotique bidimensionnelle de Mellor, donnant en particulier les principales caracteristiques qu'un modele tridimensionnel pour la loi de la paroi et pour la loi du sillage doit posseder. Proposition de lois composites paroi et sillage, en utilisant une approche vectorielle dans la region du sillage. Comparaison de ce modele avec les resultats de van den Berg et al. et ceux de Mueller, un bon accord etant constate entre la theorie et l'experience
The Pairing Game: A Classroom Demonstration of the Matching Phenomenon:

This article describes 2 versions of an in-class simulation that allows students to directly experience the matching phenomenon and explore issues concerning mate selection, social exchange, and related psychological concepts. Students are randomly assigned a value (either a numerical value or a list of adjectives), which they place on their forehead so others can see it but the student cannot. The goal is to pair off with another student with as high a value as possible. The simulation, called the Pairing Game, illustrates how matching on similarity can occur, even in the absence of knowledge of one's own value and merely by seeking the highest value possible in a partner. Students in Development Psychology courses responded favorably to the simulation on an anonymous survey.
Integration of fuel conservative procedures in the high density terminal area

Fuel conservative procedures, including profile descents and decelerating approaches, can reduce fuel consumption significantly when executed as planned, but their effectiveness is strongly influenced by the Air Traffic Control (ATC) environment. Real-time pilot-and-controller-in-the-loop simulations have been used to establish the fuel savings potential as well as the operational problems of these procedures in the current ATC system. Simulation results have shown that fuel savings are realized under moderately heavy traffic conditions. In heavy traffic, high controller workload together with reduced fuel savings indicate that time-controlled (4D) guidance in the aircraft and computer-assisted scheduling may be necessary to realize the full potential of fuel conservative procedures. A computer-assisted technique, termed Constrained Position Shifting (CPS), is proposed to integrate these procedures into the future ATC system. CPS also increases capacity and reduces delay by accounting for the differences in aircraft sizes, capabilities and routes.
pH and Down Load Effects on Silicon Dioxide Dielectric CMP

Understanding the pH and down pressure effects is critical in elucidating the chemical and mechanical mechanisms in chemical mechanical polishing (CMP). This paper describes the variation in polishing rate by nonagglomerated silicon dioxide particles. The repulsive interaction force, solubility of amorphous silica, and total contact area at the pad-particles-wafer interface are important factors in determining polishing performance. In situ friction force measurements are used to detect the variation of interfacial contact during polishing. Surface finishes and interaction force of silica/silica were investigated using atomic force microscopy.
Culekinin depolarizing peptide: a mosquito leucokinin-like peptide that influences insect Malpighian tubule ion transport

A peptide termed culekinin depolarizing peptide (CDP) was isolated from approximately 1.2 million mosquitos (94% Culex salinarius). The peptide was isolated on the basis of a rapid myotropic assay that utilized a hindgut preparation from Leucophaea maderae and a transepithelial voltage assay that used mosquito Malpighian tubules from Aedes aegypti. A 15% trifluoroacetic acid extraction from the mosquitos, two solid phase extraction steps, and six HPLC steps resulted in the isolation of 9.7 nmol of CDP. This value corresponds to approximately 8 fmol/mosquito. Edman degradation indicated the following sequence for CDP: Asn-Pro-Phe-His-Ser-Trp-Gly-NH2. The sequence was confirmed as the suspected C-terminal amide form of the peptide, since native and synthetic CDP had identical chemical and biological properties. CDP is a member of the leucokinin family of neuropeptides. The leucokinins have been found in three other insect species (Leucophaea maderae, Acheta domesticus and Locusta migratoria) where these peptides were isolated by their myotropic properties alone. CDP shares a C-terminal sequence homology (i.e., Phe-X-Ser-Trp-Gly-NH2) with the rest of the leucokinins. CDP corresponds to the strongest tubule depolarizing activity in the C. salinarius extract. These findings agree with previous structure-activity studies that suggest that mosquitos would contain a leucokinin-like factor that had Phe-His-Ser-Trp-Gly-NH2 as the C-terminal pentapeptide. This is the first leucokinin isolated from blood feeding or holometabolous insects.
Cardiovascular actions of 2,5-dimethoxy-4-methylamphetamine (DOM) in the cat.

The cardiovascular effects of the psychotomimetic DOM were investigated in pentobarbital-anesthetized cats. DOM (1,2 and 4 mg/kg, i.v.) produced a rise in mean blood pressure accompanied by bradycardia. DOM (1 and 2 mg/kg, i.v.) also produced a contraction of the decentralized nictitating membrane. The duration of the pressor response to DOM was reduced in magnitude and duration by hexamethonium, by spinal section plus vagotomy, and by carotid sinus denervation plus vagotomy. Ventricular--cisternal perfusion of 20 mug DOM resulted in a slow developing hypertensive response. Methylsergid significantly attenuated the pressor response and the contraction of the nictitating membrane induced by i.v. DOM administration. The pressor response to DOM was not altered by phentolamine, propranolol or atropine and vagotomy. Bradycardia induced by DOM was antagonized by carotid sinus denervation plus vagotomy, hexamethonium, spinal sectioning plus vagotomy, and propranolol. Bradycardia induced by DOM is therefore a result of a reflex response to increased blood pressure. The present data thus indicate that the pressor response to DOM consists of two compents: a direct vascular effect mediated via the stimulation of serotonergic receptors; and a second pressor effect, mediated by the central nervous system.
Oxidative cleavage of α-diols, α-diones, α-hydroxy-ketones and α-hydroxy- and α-keto acids with calcium hypochlorite [Ca(OCl)2].

Abstract α-Diols, α-diones, α-hydroxy ketones, and α-hydroxy- and α-keto acids are easily cleaved oxidatively with calcium hypochlorite. The reaction is carried out at ambient temperature in aqueous acetonitrile/acetic acid solution. The yields are good to excellent and the products, depending on the starting material, are aldehydes, ketones or acids.
RNA-protein interactions in unstable microsatellite diseases

Abstract A novel RNA-mediated disease mechanism has emerged from studies on dominantly inherited neurological disorders caused by unstable microsatellite expansions in non-coding regions of the genome. These non-coding tandem repeat expansions trigger the production of unusual RNAs that gain a toxic function, which involves the formation of RNA repeat structures that interact with, and alter the activities of, various factors required for normal RNA processing as well as additional cellular functions. In this review, we explore the deleterious effects of toxic RNA expression and discuss the various model systems currently available for studying RNA gain-of-function in neurologic diseases. Common themes, including bidirectional transcription and repeat-associated non-ATG (RAN) translation, have recently emerged from expansion disease studies. These and other discoveries have highlighted the need for further investigations designed to provide the additional mechanistic insights essential for future therapeutic development. This article is part of a Special Issue entitled RNA Metabolism 2013.
Changing organizational culture with scenario planning

Abstract This paper explores scenario planning as a tool to help change organizational culture. Changing the culture of the organization to adapt to changes in the environment is an important concern for most organizations—yet this change is often reactive. The authors present scenario planning as a proactive tool for developing alternative, possible cultures as an initial step toward effecting change. The following describes and clarifies the relationships between scenario planning and the cultural framework, along with research suggestions to determine the validity of this argument.