cssi/SciSciGPT-SciSciNet huggingface dataset:
Electronic structure, absorption coefficient, and Auger rate in HgCdTe and thallium-based alloys

The band structures, absorption coefficients, and Auger recombination rates in narrow-gap alloys HgCdTe, InTIP, InTlAs, and InTlSb in the zinc blende structure, along with those of GaAs, are calculated using a hybrid pseudopotential and tight-binding method. The composition-dependent band gaps of the thallium-based alloys are reported along with those of several other semiconductor alloys. Within 50 meV from the absorption edge, the absorption coefficient of InxTl1−xP is found to have about the same magnitude as that of HgxCd1−xTe and GaAs, while that of InxTl1−xAs and InxTl1−xSb is much smaller. In agreement with previous theories, the calculated Auger lifetimes in Hg0.78Cd0.22Te with unit or k • p overlap agree very well with experiments. Among the thallium alloys studied, the Auger lifetimes are longest in In0.33Tl0.67P, but still shorter than those in Hg0.78Cd0.22Te by an order of magnitude. In addition, realistic overlaps produce lifetimes one to two orders of magnitude larger than those observed.
Novel isoforms of intracellular platelet activating factor acetylhydrolase (PAFAH1b2) in human testis; encoded by alternatively spliced mRNAs.

Abstract Platelet activating factor acetylhydrolase (paf-ah), a potent regulator of platelet activating factor activity, plays an important role in various physiological and pathophysiological functions including development, reproduction, inflammation, hemostasis, and apoptosis. Intracellular paf-ah (paf-ah-Ib) is composed of a regulatory subunit, Pafah1b1, and two highly conserved but non-identical catalytic subunits, Pafah1b2 and Pafah1b3. The present study identifies new splice variants of the Pafah1b2 gene transcript. The splice variants retain exons 1–5 and replace exon 6 with alternative exons derived from genomic sequence 3′ to exon 6. Splice variants encode two proteins with different novel carboxy termini. One of the isoforms is expressed exclusively in testis. These new isoforms of pafah1b2 retain the ability to form higher order complexes while replacing known key catalytic residues, which raises the possibility that they may alter the subunit composition and catalytic function of paf-ah-Ib.
Nucleotide sequence of the envelope protein genes of a highly virulent, neurotropic strain of Newcastle disease virus.

Abstract The envelope glycoproteins of Newcastle disease virus (NDV), hemagglutinin-neuraminidase (HN) and fusion (F) proteins, play important roles in determining the host immune response and the virulence of that particular virus strain. The complete nucleotide sequence of the HN and F genes of a highly neurovirulent strain of NDV (Texas G. B., 1948) was determined in an effort to study the molecular basis of this strain's neurotropic properties. Comparison of the predicted amino acid sequences for the HN and F among the American NDV strains revealed that the Texas G. B. and Beaudette C envelope genes are closely related to each other and are less closely related to the avirulent B, Hitchner strain. We have found 11 amino acid changes in the predicted HN protein between the Beaudette C and Texas G. B. strain but only 2 conservative amino acid changes (amino acids 11 and 197) in the F protein between these two strains. Although the virulence of NDV strains has been related to sequences at the cleavage site of F 0 , the property of neurovirulence cannot depend solely upon these sequences because there are no sequence differences between the Beaudette C and Texas G. B. strains. We suggest that the neurovirulence phenotype could be due to the molecular properties of the HN protein; however, we cannot exclude the possibility that the two conservative amino acid differences between the two F proteins could also play a role in determining the phenotypic differences between these two virus strains.
Use of Insect Repellents for Dispersing Defending Honey Bees (Hymenoptera: Apidae)

Some ecotypes of the honey bee, Apis mellifera L., show excessive levels of colony defense that have occasionally resulted in human and animal deaths. In cases where death has occurred, the victim, animal or human, has often been confined or panicked into an area from which it cannot escape. Our study was done to evaluate the use of repellents to reduce the severity of the stinging during accidental disturbances of excessively defensive colonies. Three mosquito repellents (diethyl-meta-toluamide, 2-ethyl-1,3-hexandiol, and dimethyl phthlate) and 2 odiferous compounds known to be repellent to honey bees (benzaldehyde and menthol) were tested in European (Texas) and Africanized (Mexico) apiaries by victims in protective clothing. When sprayed as an aerosol at the defending worker bees, all the compounds significantly reduced the number of bees around the victim and the number of stings in a patch of suede exposed during the test. DEET was consistently the most effective repellent. A number of materials could be developed as repellents for emergency use by individuals that are at high risk of encountering wild honey bee colonies in the course of their daily activity.
5. IMPERIAL SPACES IN PEKKA HÄMÄLÄINEN'S THE COMANCHE EMPIRE

This review focuses on Pekka Hamalainen's characterization and analysis of the Comanche empire as a spatial category in The Comanche Empire and discusses how this work relates to broader discussions about space and power in borderlands and imperial histories. Although empires have long been central actors in borderlands histories, “empire” has not necessarily been a category of spatial organization and analysis and certainly not one used to describe spaces controlled by Native peoples. By contrast, while Hamalainen emphasizes the imperial characteristics of the economic, political, and cultural dimensions of Comanche history (as other contributors to this forum discuss), he also uses “empire” to characterize Comanche dominance spatially. Hamalainen helps us to rethink the spatial dynamics that both shaped and were produced by the encounters between Comanches and Spaniards, French, Mexicans, Americans, and other Native peoples in the Great Plains during the eighteenth and nineteenth centuries. By analyzing how Comanches came to control vast stretches of the southern plains, The Comanche Empire challenges our assumptions about how Native polities and imperial powers (and groups like the Comanches that Hamalainen argues were both) thought about territorial claims and how they employed more nuanced spatial strategies to assert their authority, extend their cultural influence, and control trade and resources.
Oxidation of Free L-histidine by tert -Butylhydroperoxide

L-histidine, a commonly used buffer for protein formulations, has the potential to oxidize and form multiple byproducts. Previous studies were performed using metal catalyzed oxidation with Fe2+ or Cu2+. We re-examined the oxidation of L-histidine under conditions more appropriate to protein formulations. Solutions of free L-histidine, protected from light, were initially reacted with tert-butylhydroperoxide and the products analyzed by UV absorption spectroscopy, reversed phase HPLC and mass spectrometric analysis and NMR. Experimental parameters investigated were oxidizing agent, pH, temperature, metal ion and metal chelator content. The initial reaction produced a number of known products, along with an unknown product that was identified as 4(5)-imidazolecarboxaldehyde. The reaction was highly pH and oxidizing-agent specific. The product was not observed at pH 5.0 or below, while there was a dramatic increase for reactions carried out at pH 6.0 or above. Addition of FeSO4 to the reaction dramatically increased the amount of 4(5)-imidazolecarboxaldehyde produced, while addition of the metal chelators EDTA or DTPA completely inhibited the reaction. The presence of oxidants and trace concentrations of metal ions in high purity L-histidine solutions results in the formation of 4(5)-imidazolecarboxaldehyde which has the potential to covalently modify proteins.
Does Stone Removal Help Patients with Recurrent Urinary Tract Infections

Purpose: We evaluated the impact of surgical extraction of nonobstructing asymptomatic stones on recurrent urinary tract infections and identified predictors of patients who may be rendered infection-free.Materials and Methods: We retrospectively reviewed charts to identify patients with recurrent urinary tract infections who underwent surgical stone extraction and were rendered stone-free. Demographic variables as well as procedure, infectious etiology, stone composition and the systemic inflammatory response syndrome rate were also recorded. Patients were divided into 2 groups. Group 1 had no evidence of recurrent infection following surgery while recurrent infection developed in group 2. Univariate analysis was performed using the Wilcoxon signed rank and Fisher exact tests. Logistic regression was used for multivariate analysis.Results: We identified 120 patients with recurrent urinary tract infections and a nonobstructive renal stone. Surgical management included shock wave lithotripsy in 32% of case...
A Priori Open Loop Optimal Control of Continuous Time Stochastic Systems

ABSTRACT This paper is presented, not so much as an end in itself concerning the solution of a definite engineering problem, but as an exploratory step toward the solution of the problem of optimal control of continuous time stochastic non-linear systems when only noisy observations of the state are available. In this paper, the problem of determining the optimal open loop control, when no observations at all are available, is treated by dynamic programming. The main results of the paper are to obtain the functional equation of dynamic programming and to present a quasi-linearization type of algorithm for its solution. The author's intention was to illustrate by these results that infinite dimensional function space is the most natural setting for stochastic non-linear problems. The results obtained give some insight into what can be expected in the more general case of noisy observations. In the Appendices an argument is presented to justify the proposed algorithm and an example is given for which an exa...
Mutual Event Observations of Io’s Sodium Corona

We have measured the column density profile of Io's sodium corona using 10 mutual eclipses between the Galilean satellites. This approach circumvents the problem of spatially resolving Io's corona directly from Io's bright continuum in the presence of atmospheric seeing and telescopic scattering. The primary goal is to investigate the spatial and temporal variations of Io's corona. Spectra from the Keck Observatory and McDonald Observatory from 1997 reveal a corona that is only approximately spherically symmetric around Io. Comparing the globally averaged radial sodium column density profile in the corona with profiles measured in 1991 and 1985, we find that there has been no significant variation. However, there appears to be a previously undetected asymmetry: the corona above Io's sub-Jupiter hemisphere is consistently more dense than above the anti-Jupiter hemisphere.
Fermentation Temperature Modulates Phosphatidylethanolamine and Phosphatidylinositol Levels in the Cell Membrane of Saccharomyces cerevisiae

During alcoholic fermentation, Saccharomyces cerevisiae is exposed to a host of environmental and physiological stresses. Extremes of fermentation temperature have previously been demonstrated to induce fermentation arrest under growth conditions that would otherwise result in complete sugar utilization at "normal" temperatures and nutrient levels. Fermentations were carried out at 15°C, 25°C, and 35°C in a defined high-sugar medium using three Saccharomyces cerevisiae strains with diverse fermentation characteristics. The lipid composition of these strains was analyzed at two fermentation stages, when ethanol levels were low early in stationary phase and in late stationary phase at high ethanol concentrations. Several lipids exhibited dramatic differences in membrane concentration in a temperature-dependent manner. Principal component analysis (PCA) was used as a tool to elucidate correlations between specific lipid species and fermentation temperature for each yeast strain. Fermentations carried out at 35°C exhibited very high concentrations of several phosphatidylinositol species, whereas at 15°C these yeast strains exhibited higher levels of phosphatidylethanolamine and phosphatidylcholine species with medium-chain fatty acids. Furthermore, membrane concentrations of ergosterol were highest in the yeast strain that experienced stuck fermentations at all three temperatures. Fluorescence anisotropy measurements of yeast cell membrane fluidity during fermentation were carried out using the lipophilic fluorophore diphenylhexatriene. These measurements demonstrate that the changes in the lipid composition of these yeast strains across the range of fermentation temperatures used in this study did not significantly affect cell membrane fluidity. However, the results from this study indicate that fermenting S. cerevisiae modulates its membrane lipid composition in a temperature-dependent manner.
How Well Is CHIP Addressing Health Care Access and Affordability for Children

This paper examines how access to care and care experiences under the Children's Health Insurance Program (CHIP) compared to private coverage and being uninsured in 10 states, with findings drawn from a 2012 survey. Children with CHIP coverage had substantially better access to care across a range of outcomes including specialty and mental health services, other things being equal, particularly compared to those with no coverage. Compared to being privately insured, CHIP enrollees had generally similar health care use and unmet needs, and lower financial burden. However, we found room for improvement in CHIP enrollees' access to care.
Effects of dietary esfenvalerate exposures on three aquatic insect species representing different functional feeding groups.

Given the chemical properties of synthetic pyrethroids, it is probable that compounds, including esfenvalerate, that enter surface waters may become incorporated into aquatic insect food sources. We examined the effect of dietary esfenvalerate uptake in aquatic insects representing different functional feeding groups. We used three field-collected aquatic insect species: A grazing scraper, Cinygmula reticulata McDunnough (Ephemeroptera: Heptageniidae); an omnivorous filter feeder, Brachycentrus americanus Banks (Trichoptera: Brachycentridae); and a predator, Hesperoperla pacifica Banks (Plecoptera: Perlidae). Laboratory-cultured algae were preexposed for 24 h to esfenvalerate concentrations of 0, 0.025, 0.05, and 0.1 μg/L and provided to two C. reticulata age classes (small and final-instar nymphs). Reduction in small nymph growth was observed following three weeks of feeding on algae exposed to 0.05 and 0.1 μg/L of esfenvalerate, and the highest dietary exposure reduced egg production in final-instar nymphs. The diet for B. americanus and H. pacifica consisted of dead third-instar Chironomus tentans larvae preexposed for 24 h to esfenvalerate concentrations ranging between 0.1 and 1.0 μg/L. Consumption of larvae exposed to 0.5 to 1.0 μg/L of esfenvalerate caused case abandonment and mortality in B. americanus caddisfly larvae. Although H. pacifica nymphs readily consumed esfenvalerate-exposed larvae, no adverse effects were observed during the present study. Furthermore, no evidence of esfenvalerate-induced feeding deterrence was found in any of the species tested, suggesting that aquatic insects may not be able to distinguish between pyrethroid-contaminated and uncontaminated food sources. These findings indicate that feeding deterrence is not a factor in regulating aquatic insect dietary exposures to synthetic pyrethroids.
Diagnostic Value of Immunocytochemistry in Leptomeningeal Tumor Dissemination

Differentiating chronic aseptic meningitis from leptomeningeal carcinomatosis or gliomatosis can be difficult, particularly when the differentiation is based solely on routine cytologic examination. The diagnosis of cerebrospinal fluid tumor dissemination in at-risk patients requires cytologic examination of cerebrospinal fluid and radiography of the leptomeninges. Routine cytologic examination alone has proven less than desirable, in most instances providing confirmation in as little as 50% of cases in the first lumbar puncture. This percentage increases to 85% to 90% after multiple lumbar punctures. We retrospectively reviewed 2 cases of leptomeningeal dissemination (one gliomatosis, the other carcinomatosis) with initial false-negative test results. However, after further examination of the cerebrospinal fluid by selected battery of immunocytochemical stains, both cases were identified as positive for malignancy (ie, false negatives). Immunocytochemistry can be useful in distinguishing chronic aseptic meningitis from leptomeningeal carcinomatosis or gliomatosis in patients at risk or when abnormal cells are seen on routine cerebrospinal fluid cytologic examination.
The in vivo evaluation of hand/rotary/ultrasound instrumentation in necrotic, human mandibular molars

This in vivo, prospective, randomized, single-blinded study histologically compared biofilm/necrotic debridement efficiency of a hand/rotary technique versus a hand/rotary/1 min ultrasound technique in the mesial roots of necrotic, human mandibular molars. The hand/rotary group consisted of 20 mesial roots. The hand/rotary/ultrasound group consisted of 20 mesial roots prepared with the same hand/rotary technique followed by 1 min of ultrasonic irrigation, per canal, utilizing an ultrasonic needle in a MiniEndo unit. Following extraction, histologic preparation and staining, 0.2 mum cross-sections from the 1- to 3-mm apical levels were evaluated for percentage of biofilm/necrotic debris removal. Cleanliness results at the 1-, 2- and 3-mm levels for the hand/rotary and hand/rotary/ultrasound techniques, respectively, were: Canals, 80% versus 95%, 92% versus 99%, and 95% versus 100%; Isthmuses, 33% versus 83%, 31% versus 86%, 45% versus 91%. Statistical analysis revealed mean percent canal and isthmus cleanliness values to be significantly higher for hand/rotary/ultrasound technique at all levels evaluated.
Formation of chlorins and meso-substituted porphyrins through intramolecular nitrogen-carbon migration of N-substituted porphyrins

A series of N-substituted porphyrins were synthesized either by direct alkylation of the symmetrical porphyrins or from the unsymmetrical porphyrins obtained via MacDonald's procedure using the appropriate N-substituted dipyrromethanes. Depending upon the nature of the peripheral substitutions in the porphyrin system, the nickel(II)-promoted rearrangement of these compounds gave either meso-substituted or unsubstituted Ni(II) porphyrins or chlorins. The chlorins and meso-substituted porphyrins were formed via the intramolecular migration of the N-substituent to carbon. This approach provides easy access for the synthesis of chlorins and the meso-substituted porphyrins, which are otherwise difficult to synthesize.
Management of airway manifestations of relapsing polychondritis: case reports and review of literature.

Study objective To report the first series of patients with severe airway manifestations of relapsing polychondritis (RP) that were managed successfully with self-expandable metallic stents, and to review the literature. Design Retrospective review of medical records, and current clinical follow-up. Setting Tertiary care referral hospital. Patients All patients with airway manifestations of RP that were managed with self-expandable metallic stents at our institution. Results All five patients (four women and one man; age, 40 to 69 years old) had severe airway manifestations, and three of them required mechanical ventilation. Spirometry with flow-volume curves showed severe combined obstructive and restrictive ventilatory defects. Bronchoscopy revealed dynamic collapse of the proximal airways. Diagnosis was made 8 months to 13 years after the first symptom of the disease. Pharmacotherapy included prednisone, methotrexate, cyclosporine, and dapsone. A total of 17 self-expandable metallic stents of varying sizes were placed using flexible bronchoscope from 4 to 19 years after the first symptom. The overall outcome was favorable in four patients. Three patients have survived without ventilatory support 16 to 18 months following the first stent placement, and the fourth patient survived for 20 months without ventilatory support before she died. The fifth patient, who was receiving mechanical ventilation, died in 1 week probably due to persistent dynamic collapse of the airways distal to the stents. Conclusion Self-expandable metallic tracheobronchial stents should be considered in the management of airway manifestations of RP, especially in patients who require mechanical ventilation.
Salmonella enterica Virulence Genes Are Required for Bacterial Attachment to Plant Tissue

Numerous Salmonella enterica food-borne illness outbreaks have been associated with contaminated vegetables, in particular sprouted seeds, and the incidence of reported contamination has steadily risen. In order to understand the physiology of S. enterica serovar Newport on plants, a screen was developed to identify transposon mutants that were defective in attachment to alfalfa sprouts. Twenty independent mutants from a pool of 6,000 were selected for reduced adherence to alfalfa sprouts. Sixty-five percentage of these mutants had insertions in uncharacterized genes. Among the characterized genes were strains with insertions in the intergenic region between agfB, the surface-exposed aggregative fimbria (curli) nucleator, and agfD, a transcriptional regulator of the LuxR superfamily, and rpoS, the stationary-phase sigma factor. Both AgfD and RpoS have been reported to regulate curli and cellulose production and RpoS regulates other adhesins such as pili. The intergenic and rpoS mutants were reduced in initial attachment to alfalfa sprouts by 1 log unit compared to the wild type. Mutations of agfA, curli subunit, and agfB in S. enterica serovar Enteritidis differentially affected attachment to plant tissue. The agfA mutation was not reduced in ability to attach to or colonize alfalfa sprouts, whereas the agfB mutation was reduced. Thus, agfB alone can play a role in attachment of S. enterica to plant tissue. These results reveal that S. enterica genes important for virulence in animal systems are also required for colonization of plants, a secondary host that can serve as a vector of S. enterica from animal to animal.
Prospective Comparative Study of Variable Dosage and Variable Frequency Regimens for Administration of Gentamicin

In patients with impaired renal function, careful adjustment of gentamicin dosage is required to achieve therapeutic yet nontoxic concentrations. Two regimens that differ in pharmacodynamic characteristics have been recommended for this purpose: prolonging the intervals between administration of equal doses (variable frequency regimen [VFR]) or administering a loading dose followed at the usual intervals by reduced maintenance doses (variable dosage regimen [VDR]). These regimens were compared in a prospective, randomized study of 20 seriously ill hospitalized patients, 10 on VFR and 10 on VDR. Wide variability in peak serum levels of gentamicin was observed both between patients and in individual patients after separate injections of the same dosage. As predicted by the design of these regimens, the trough serum levels of gentamicin correlated significantly with the serum creatinine concentrations in patients on the VDR but not in patients on the VFR. A gentamicin trough level of >/=4 mug/ml was the only variable among those tested that correlated significantly with development or progression of renal insufficiency during treatment with gentamicin, but such trough levels were observed frequently on both regimens. Whereas this study does not permit a direct comparison of the therapeutic efficacy of VDR and VFR, no difference in the risk of nephrotoxicity with these regimens was observed.
Minireview: Inhibin binding protein (InhBP/p120), betaglycan, and the continuing search for the inhibin receptor

Betaglycan (the TGFβ type III receptor) and InhBP/p120 (a membrane-tethered proteoglycan) were recently identified as putative inhibin receptors. Here, we review the current state of knowledge regarding these two proteins with respect to their potential roles in inhibin biology. Importantly, neither protein appears to satisfy all of the criteria required for classification as a bona fide inhibin receptor. Betaglycan does not appear to be expressed in pituitary gonadotropes, the primary target of circulating inhibins, and InhBP/p120 does not bind inhibins in conventional receptor binding assays. While both proteins appear capable of promoting inhibin-mediated antagonism of activin signaling, neither appears to generate inhibin-specific intracellular signals. Recently, additional inhibin binding proteins were identified in inhibin target tissues, including pituitary and Leydig cells. Characterization of these proteins, coupled with ongoing investigations of betaglycan and InhBP/p120, will lead to a clearer ...
Foliations Leaf Through Economics

Foliations provide a general, convenient, geometric way to catalogue information from topics as varied as sufficient statistics, solutions of differential equations, indifference curves for utility functions, distributed computing, and so forth. An introduction to aspects of this area is provided.
Pre-steady-state Kinetic Analysis of a Family D DNA Polymerase from Thermococcus sp. 9°N Reveals Mechanisms for Archaeal Genomic Replication and Maintenance

Family D DNA polymerases (polDs) have been implicated as the major replicative polymerase in archaea, excluding the Crenarchaeota branch, and bear little sequence homology to other DNA polymerase families. Here we report a detailed kinetic analysis of nucleotide incorporation and exonuclease activity for a Family D DNA polymerase from Thermococcus sp. 9°N. Pre-steady-state single-turnover nucleotide incorporation assays were performed to obtain the kinetic parameters, kpol and Kd, for correct nucleotide incorporation, incorrect nucleotide incorporation, and ribonucleotide incorporation by exonuclease-deficient polD. Correct nucleotide incorporation kinetics revealed a relatively slow maximal rate of polymerization (kpol ∼2.5 s−1) and especially tight nucleotide binding (Kd(dNTP) ∼1.7 μm), compared with DNA polymerases from Families A, B, C, X, and Y. Furthermore, pre-steady-state nucleotide incorporation assays revealed that polD prevents the incorporation of incorrect nucleotides and ribonucleotides primarily through reduced nucleotide binding affinity. Pre-steady-state single-turnover assays on wild-type 9°N polD were used to examine 3′-5′ exonuclease hydrolysis activity in the presence of Mg2+ and Mn2+. Interestingly, substituting Mn2+ for Mg2+ accelerated hydrolysis rates >40-fold (kexo ≥110 s−1 versus ≥2.5 s−1). Preference for Mn2+ over Mg2+ in exonuclease hydrolysis activity is a property unique to the polD family. The kinetic assays performed in this work provide critical insight into the mechanisms that polD employs to accurately and efficiently replicate the archaeal genome. Furthermore, despite the unique properties of polD, this work suggests that a conserved polymerase kinetic pathway is present in all known DNA polymerase families.
Unconventional Myosins in Cell Movement, Membrane Traffic, and Signal Transduction

In the past few years genetic, biochemical, and cytolocalization data have implicated members of the myosin superfamily of actin-based molecular motors in a variety of cellular functions including membrane trafficking, cell movements, and signal transduction. The importance of myosins is illustrated by the identification of myosin genes as targets for disease-causing mutations. The task at hand is to decipher how the multitude of myosins function at both the molecular and cellular level-a task facilitated by our understanding of myosin structure and function in muscle.
The Many Layers of Specification and Plasticity in the Neocortex

In this issue of Neuron, Li et al. (2013) show that transgenically eliminating thalamocortical neurotransmission disrupts the formation of barrel columns in the somatosensory cortex and cortical lamination, providing evidence for the importance of extrinsic activity-dependent factors in cortical development.
Transcription factor binding element detection using functional clustering of mutant expression data

As a powerful tool to reveal gene functions, gene mutation has been used extensively in molecular biology studies. With high throughput technologies, such as DNA microarray, genome-wide gene expression changes can be monitored in mutants. Here we present a simple approach to detect the transcription-factor-binding motif using microarray expression data from a mutant in which the relevant transcription factor is deleted. A core part of our approach is clustering of differentially expressed genes based on functional annotations, such as Gene Ontology (GO). We tested our method with eight microarray data sets from the Rosetta Compendium and were able to detect canonical binding motifs for at least four transcription factors. With the support of chromatin IP chip data, we also predict a possible variant of the Swi4 binding motif and recover a core motif for Arg80. Our approach should be readily applicable to microarray experiments using other types of molecular biology techniques, such as conditional knockout/overexpression or RNAi-mediated ‘knockdown’, to perturb the expression of a transcription factor. Functional clustering included in our approach may also provide new insights into the function of the relevant transcription factor.
"I'd Know a False Confession if I Saw One": A Comparative Study of College Students and Police Investigators

College students and police investigators watched or listened to 10 prison inmates confessing to crimes. Half the confessions were true accounts; half were false—concocted for the study. Consistent with much recent research, students were generally more accurate than police, and accuracy rates were higher among those presented with audiotaped than videotaped confessions. In addition, investigators were significantly more confident in their judgments and also prone to judge confessors guilty. To determine if police accuracy would increase if this guilty response bias were neutralized, participants in a second experiment were specifically informed that half the confessions were true and half were false. This manipulation eliminated the investigator response bias, but it did not increase accuracy or lower confidence. These findings are discussed for what they imply about the post-interrogation risks to innocent suspects who confess.
Sculpting God: An Exchange (1)

In a recent issue of the Harvard Theological Review, John N. Jones argues that one must radically reinterpret the status of negative theology in pseudo-Dionysius.' Unlike previous interpretations of pseudo-Dionysius, his article claims that this negative speech about God, which calls into question all positive claims regarding the divine, does not refute its own claims. Previous readings have tended to treat all negative language synonymously. If, therefore, pseudo-Dionysius negates all negative language, including the negation of negative language, his thought would be paradoxical and self-negating. Jones's crucial observation is that pseudo-Dionysius actually uses three categories of negative language: the denial of all being, particular denials, and negations. By distinguishing among these categories, Jones attempts to show that pseudo-Dionysius does not deny indiscriminately the use of all language for the transcendent God, but only that language implying existence, including particular denials and assertions. One can distinguish such language from the denial of all beings. This is indicated by negation and negations.2 Jones's essay is a sustained attempt to examine the possibility of self-negation in pseudo-Dionysius and one of the few attempts to delineate various modes of negative language in his thought-a delineation surely necessary, considering that Neoplatonist philosophers explicitly recognized how varying kinds of negative language function differently. Jones overlooks a key exegetical point, however, and this calls into question his overall thesis. Jones argues that in the second chapter of The Celestial Hierarchy, pseudo-Dionysius identifies words for God that
Characterization of aromatic organosulfur model compounds relevant to fossil fuels by using atmospheric pressure chemical ionization with CS2 and high-resolution tandem mass spectrometry.

Rationale The chemistry of desulfurization involved in processing crude oil is greatly dependent on the forms of sulfur in the oil. Sulfur exists in different chemical bonding environments in fossil fuels, including those in thiophenes and benzothiophenes, thiols, sulfides, and disulfides. In this study, the fragmentation behavior of the molecular ions of 17 aromatic organosulfur compounds with various functionalities was systematically investigated by using high-resolution tandem mass spectrometry. Methods Multiple-stage tandem mass spectrometric experiments were carried out using a linear quadrupole ion trap (LQIT) equipped with an atmospheric pressure chemical ionization (APCI) source. (+)APCI/CS2 was used to generate stable dominant molecular ions for all the compounds studied except for three sulfides that also showed abundant fragment ions. The LQIT coupled with an orbitrap mass spectrometer was used for elemental composition analysis, which facilitated the identification of the neutral molecules lost during fragmentation. Results The characteristic fragment ions generated in MS(2) and MS(3) experiments provide clues for the chemical bonding environment of sulfur atoms in the examined compounds. Upon collision-induced dissociation (CID), the molecular ions can lose the sulfur atom in a variety of ways, including as S (32 Da), HS(•) (33 Da), H2 S (34 Da), CS (44 Da), (•) CHS (45 Da) and CH2 S (46 Da). These neutral fragments are not only indicative of the presence of sulfur, but also of the type of sulfur present in the compound. Generally, losses of HS(•) and H2 S were found to be associated with compounds containing saturated sulfur functionalities, while losses of S, CS and (•) CHS were more common for heteroaromatic sulfur compounds. Conclusions High-resolution tandem mass spectrometry with APCI/CS2 ionization is a viable approach to determining the types of organosulfur compounds. It can potentially be applied to analysis of complex mixtures, which is beneficial to improving the desulfurization process of fossil fuels. Copyright © 2016 John Wiley & Sons, Ltd.
Overwintering Survival of the Sunflower Stem Weevil (Coleoptera: Curculionidae) and Two of Its Parasitoids in the Northern Great Plains

Overwintering survival of the sunflower stem weevil (SSW), Cylindrocopturus adspersus (LeConte), varied with microhabitat of the larvae. Survival was significantly higher for larvae protected within the sunflower stalk compared with larvae exposed in the soil. Survival ranged from 11.9 to 68.4%, 9.2 to 58.6%, and 3.7 to 33.2% during the fall-winter seasons of 1981-1982, 1982-1983, and 1983-1984, respectively. Nealiolus curculionis (Fitch) and Tetrastichus ainsliei Gahan parasitized 10.6, 7.0, 29.8%, and 0.6, 1.2, 0.3% of the SSW larvae recovered from stalks collected in the fall in 1981-1982, 1982-1983, and 1983-1984, respectively. Microhabitats favoring SSW survival also favored parasitoid survival.
Immunohistochemical Progesterone Receptor Assay: Measurement by Image Analysis

To determine the efficiency of image analysis in immunohistochemical progesterone receptor (PgR) measurement, 94 primary breast carcinoma tissue samples were evaluated for PgR by biochemical dextran-coated charcoal assay (DCC) and an immunohistochemical method. Frozen sections immunostained for PgR with a monoclonal antibody (Abbott PgR-ICA, Chicago, IL) and the peroxidase-antiperoxidase technique were scored semiquantitatively histologic score by microscopy and quantitatively (percentage nuclear area immunopositivity [PNA] using the CAS 200 image analyzer (Cell Analysis Systems, Elmhurst, IL). There was a positive correlation between dextran-coated charcoal assay and both histologic score (r = 0.82) and PNA (r = 0.69). Selected cutoff points of 60 histologic score and 6.5% PNA based on sensitivity/specificity calculations yielded a predictive value of a negative test of 73% and 80%, respectively, and a positive predictive value of 100% for both; ranges of fmol/mg protein PgR correspond to ranges of histologic score and PNA. The use of an image analyzer to measure PNA in PgR-immunostained sections is a viable alternative to dextran-coated charcoal assay, especially when insufficient fresh tissue is available.
Relationship of the Optic Nerve to the Medial Rectus Muscle During Endonasal Dissection of the Medial Intraconal Orbital Apex

BACKGROUND Few studies have established surgical landmarks for endoscopic endonasal dissection of the intraconal orbital apex (OA). OBJECTIVE We describe the optic nerve (ON) anatomy and its relationships, as seen during a fully endoscopic, endonasal approach to the medial intraconal OA. METHODS The study question was approached through a cadaver dissection and a radiographic study. Four formalin-fixed, latex-injected cadaver heads were dissected using transnasal endoscopic techniques. Dissection was performed using 0 degree and 30 degree nasal endoscopes and standard endoscopic sinus surgical instrumentation. A bi-nostril 4-handed technique was used. The anatomy of 8 medial OAs was evaluated and recorded. As the radiographic portion, 100 consecutively enrolled patient magnetic resonance images were evaluated, with particular attention given to the relationship of the ON to the medial rectus muscle (MRM) in 200 orbits. RESULTS Intraconally, the ON consistently coursed along the superior half of the MRM. Interestingly, the nerve was more easily identified from a superior approach after retracting the MRM inferiorly. With the identification of the nerve at the OA, carrying the dissection of the medial OA was easily accomplished with the ON as the guiding landmark. The radiographic portion of this study revealed a consistent relationship between the superior edge of the ON and the MRM. This relationship was maintained in the orbital apex in 98.4%-100% of the orbits examined. CONCLUSION The superior edge of the optic nerve is consistently found coursing along the superior half of the MRM, facilitating facile identification and further dissection navigation.
Detection of telomerase activity in high concentration of cell lysates using primer-modified gold nanoparticles

Although the telomeric repeat amplification protocol (TRAP) has served as a powerful assay for detecting telomerase activity, its use has been significantly limited when performed directly in complex, interferant-laced samples. In this work, we report a modification of the TRAP assay that allows the detection of high-fidelity amplification of telomerase products directly from concentrated cell lysates. Briefly, we covalently attached 12 nm gold nanoparticles (AuNPs) to the telomere strand (TS) primer, which is used as a substrate for telomerase elongation. These TS-modified AuNPs significantly reduce polymerase chain reaction (PCR) artifacts (such as primer dimers) and improve the yield of amplified telomerase products relative to the traditional TRAP assay when amplification is performed in concentrated cell lysates. Specifically, because the TS-modified AuNPs eliminate most of the primer-dimer artifacts normally visible at the same position as the shortest amplified telomerase PCR product apparent on agarose gels, the AuNP-modified TRAP assay exhibits excellent sensitivity. Consequently, we observed a 10-fold increase in sensitivity for cancer cells diluted 1000-fold with somatic cells. It thus appears that the use of AuNP-modified primers significantly improves the sensitivity and specificity of the traditional TRAP assay and may be an effective method by which PCR can be performed directly in concentrated cell lysates.
A case of Isolated Fracture of the Coracoid Process of the Scapula

Fractures of the coracoid process of the scapula are rare in traumatic injuries of the shoulder. A case of isolated fracture of the coracoid process is presented. The subject was a 57-years-old male who fell from a height. Axial roentgenogram showed a displaced fracture of the tip of the coracoid process. Operative treatment was performed. At the last investigation, the fracture had united, and the patient had returned to the occupation he had prior to his injury.
STATUS AND PROSPECT OF SELF-TUNING CONTROLLER BASED ON FUZZY REASONING

Status of self-tuning controller based on fuzzy reasoning is overviewed in this paper. Several problems in fuzzy control are also discussed.
Molecular characterization of a glycosylphosphatidylinositol-linked ADP- ribosyltransferase from lymphocytes

Mono ADP-ribosyltransferases catalyze the transfer of the ADP-ribose moiety of nicotinamide adenine dinucleotide (NAD) to proteins. It was reported by Wang et al (J Immunol 153:4048, 1994) that incubation of mouse cytotoxic T lymphocytes (CTL) with NAD resulted in the ADP- ribosylation of membrane proteins and inhibition of cell proliferation and cytotoxicity. Treatment of CTL with phosphatidylinositol-specific phospholipase C (PI-PLC) before incubation with NAD prevented the inhibitory effects of NAD on the cells, consistent with the removal of a glycosylphosphatidylinositol (GPI)-anchored ADP-ribosyltransferase on the lymphocyte surface. We have identified and cloned a GPI-linked ADP- ribosyltransferase from Yac-1 mouse T-cell lymphoma cells. The deduced amino acid sequence of the Yac-1 transferase was 70% and 41% identical to those of the rabbit skeletal muscle and chicken heterophil, respectively. It contained three noncontiguous sequences similar to those found in several of the bacterial toxin and vertebrate ADP- ribosyltransferases. Based on crystallography of the bacterial toxins, these regions are believed to form, in part, the catalytic site consistent with a common mechanism for the ADP-ribose transfer reaction. In rat mammary adenocarcinoma (NMU) cells transformed with the Yac-1 transferase cDNA, transferase activity was present on the cell surface and was released into the medium by treatment of cells with PI-PLC. Thus, we have cloned a novel gene that has properties identical to the transferase detected in CTL, and may be involved in the NAD-dependent regulation of proliferation and cytotoxicity.
Mentoring, Managing, and Helping: A Critical Race Analysis of Socialization in Doctoral Education

This study utilizes a critical race theory framework to explore the ways race and gender influence Black doctoral students’ socialization experiences. Themes about the varied roles and identities o...
P116 – 2582: Palliative care and paediatric neurology

Objective The high frequency of neurological processes of uncertain or incurable prognosis demands an active and global attention of the patient and his family aiming to improve the attention and the quality of life of these patients. The palliative care have to be integrated into the neurology practice. We analyzed the characteristics of pediatric neurological patients admitted to the Pediatric Home Health Care Unit of a major hospital with neurological diseases including epidemiological, diagnostic, developmental and survival data. Methods A retrospective, descriptive study of patients admitted to Home Health Care with neurological diseases for palliative care for 5 years. Results During past 5 years 52 patients were admitted with neurological disorders (10.5% of total). The mean age on admittance was 34.5 months (range 0 to 152 months). The male/female ratio was 24/28. The most frequent disorders were: 10 severe cerebral palsy with difficult to control symptoms, 8 brain tumors, 5 metabolic disorders, 6 central nervous system malformations, 4 hypoxic ischemic encephalopathies, 4 patients with sequels of extremely premature birth, 3 spinal muscular atrophies and 2 chromosomal abnormalities. Provenance was mostly hospital wards (54%), and oncology (15%) and neurology (12%) consultations. In addition to general palliative care, 41 patients required enteral nutrition, 26 requiered oxygen and 7 NIV in their homes. During follow-up, there was at least one hospital admission in 18 patients. The mean stay in the unit was 8.6 months. Of the patients admitted, 13 died, five of them at home. Conclusion Diverse neurological diseases are characterized by a lack of response to the treatment, a progressive evolution of the disease, and in certain cases, a fatal prognosis in a relatively short term. We propose, in these patients, comprehensive care at home to prevent unnecessary hospital admissions, and when the time comes help them at the end of their days
Entry Mode and Institutional Learning: A Polycentric Perspective

This chapter contributes to the existing literature on institutional theory and international business research by integrating the concepts of polycentrism and institutional learning to examine how MNEs from emerging economies invest in developed countries. We argue that equity-based market entry modes and non-equity-based modes create different needs for learning about economic, regulatory and political institutions; entry modes with or without local partners lead to different levels of institutional embeddedness and institutional learning speeds. Finally, the content of institutional knowledge also determines its transferability and adaptability. We emphasize the importance of recognizing the integrated nature of economic, regulatory and political institutions from a polycentric perspective and discuss their change in different situations.
Seeking Social Justice: Undergraduates’ Engagement in Social Change and Social Justice at American Research Universities

Service-learning has often been assumed to be inherently connected to concerns of social justice, but there are few empirical studies exploring these connections. This chapter contributes to this empirical question through exploration of how students classify their community engagement experiences and the outcomes developed in the different types of experiences. Seven campuses responded to the 2014 Student Experience in the Research University (SERU) survey. We obtained a small sub-population of students who completed a survey module related to students’ participation in community engagement activities. The data demonstrate that community engagement activities are positively associated with a number of outcomes considered essential to preparing students for active engagement in a diverse democracy. While only a small number of students classify their experiences as social justice, the results observed across classifications of community engagement (e.g., charity, empowering others, social change, social justice) suggest that the different frameworks we provide students for their community engagement work may have different impacts both inside and outside the classroom.