cssi/SciSciGPT-SciSciNet huggingface dataset:
Neuroprotection against staurosporine by metalloporphyrins independent of antioxidant capability

Abstract Metalloporphyrin catalytic antioxidants are remarkably useful in protecting cells and tissues in a wide array of disease models, attributed primarily to functioning as superoxide dismutase (SOD) mimetics or by scavenging other reactive oxygen species (ROS). However, we recently showed that neuroprotection against Ca 2+ -dependent excitotoxic insults did not correlate with antioxidant strength or capability [25] , raising the question of whether scavenging of ROS underlies neuroprotection in other types of neuronal injury. The protein kinase inhibitor staurosporine causes neuronal demise primarily by apoptosis. Neuroprotection from staurosporine by a limited number of metalloporphyrin antioxidants has previously been attributed to antioxidant action. In the current study, a wide array of anionic and cationic metalloporphyrins and porphyrins, ranging in antioxidant strength or capability, provided protection against staurosporine in cortical neuron and cerebellar granule neuron (CGN) culture. Neuroprotection did not correlate with antioxidant strength or capability. In CGN but not cortical neuron cultures, NMDA receptor antagonists also prevented neurotoxicity, so metalloporphyrins may also target this secondary mode of death induced by staurosporine. Neuroprotection observed with antioxidant-inactive controls raises the possibility of an additional, or perhaps alternative, mechanism by antioxidant analogs not involving ROS scavenging.
Osteoporosis in HIV-infected patients

Bone disorders have emerged in the last 5 yr as additional long-term complications of human immunodeficiency virus (HIV) infection and its treatment. In particular, multiple studies suggest that HIV-infected patients receiving potent antiretroviral therapy have an increased prevalence of both osteopenia and osteoporosis. Studies prior to the era of potent antiretroviral therapy suggest that HIV infection has a modest effect on bone turnover. Treatment of HIV increases bone turnover with a greater effect on bone resorption. The mechanisms for increased bone demineralization in HIV-infected patients are unclear, although there is some evidence that specific drugs may inhibit bone formation in vitro. Small studies suggest that, in general, bone loss is not progressive over a short period of follow-up, and that the bisphosphonates may be useful in management; however, larger studies are needed to confirm these findings and assess the long-term implications of bone loss in HIV-infected persons.
Hippocampal input to a "visceral motor" corticobulbar pathway: an anatomical and electrophysiological study in the rat.

The hippocampus has previously been shown to influence cardiovascular function, and this effect appears to be mediated by the connection the hippocampus has with the infralimbic area of the medial frontal cortex (MFC), a region which projects directly to the nucleus of the solitary tract (NTS) in the dorsal medulla. In the present study, anatomical and electrophysiological techniques were utilized to determine the degree of convergence of hippocampal input to the MFC on neurons in the MFC which project to the NTS. Injections of the anterograde and retrograde neuroanatomical tracer wheatgerm agglutinin-horseradish peroxidase (WGA-HRP) into the NTS retrogradely labelled cells in the infralimbic and prelimbic regions of the MFC. Injections of WGA-HRP into the ventral hippocampus anterogradely labelled terminals in the MFC which, at the light microscopic level, closely overlapped the origin of the descending projection from the MFC to the brainstem. Electron microscopic analysis revealed that anterogradely labelled terminals make synaptic contact primarily on dendritic processes in the neuropil adjacent to retrogradely labelled cells. In addition, anterogradely labelled terminals did, in some cases, make synaptic contact on the somas of retrogradely labelled cells. Electrical stimulation of the NTS antidromically activated cells in the infralimbic and prelimbic areas of the MFC. The average latency of antidromic activation was 30 msec, corresponding to a conduction velocity of approximately 0.7 m/s. Electrical stimulation of the ventral hippocampus orthodromically activated cells in the MFC. With an appropriate delay between the hippocampal and NTS stimuli, the orthodromic and antidromic potentials could be made to collide. The results of this study establish a structural as well as functional link between the hippocampus and NTS-projection neurons in the MFC.
SOME RECENT EXPERIMENTS ON DIRECT WEFT PROJECTION

This paper describes three types of experimental equipment for projecting weft by means of solid-body friction and the results of some preliminary experiments. The theory is given of the way to use two Hooke's couplings most effectively to produce a decelerated delivery with cylindrical delivery discs and several desirable design features to be borne in mind are discussed. Some experiments with conical delivery rollers are described which show the extent of the variation in the length of yarn delivered on each pick and in the deviation of the direction of projection of the yarn from the nominal. The investigation of the ancillary use of relatively low-speed air-streams has been initiated with the aid of a closed-circuit wind-tunnel in association with constant-speed yarn delivery.
Undetected iatrogenic lesions of the anterior femoral shaft during intramedullary nailing: a cadaveric study

Background The incidence of undetected radiographically iatrogenic longitudinal splitting in the anterior cortex during intramedullary nailing of the femur has not been well documented.
The anodic oxidation of ethylene on heterogeneous AuPt alloys

Abstract The anodic oxidation of ethylene on AuPt alloys has been studied at 80°C in aqueous solutions (pH 0·35–12·7). The over-all compositions of the alloys were 80, 60, 40 and 20 at-% Au. Analyses showed them to possess two-phase, α1 (Pt-rich) and α2 (Au-rich), microstructures. The effects of potential, temperature, electrolyte pH and ethylene partial pressure on cd were determined and the results interpreted in terms of separate reactions occurring on the individual phases. The reactions on the Au-rich and Pt-rich phases were similar to those on pure Au and Pt, respectively. Mathematical equations describing the reactions are presented to correlate the experimental data.
Role of balloon angioplasty in the treatment of aortic coarctation

Since the initial report of coarctation balloon angioplasty in 1982, several groups have used this technique for native coarctations in neonates, infants, and children and for postoperative recoarctations. However, recommendations for use of balloon angioplasty as a treatment procedure of choice are clouded by reports of aneurysm development at the site of coarctation. Here we review our experience as well as that published in the literature, including Valvuloplasty and Angioplasty of Congenital Anomalies Registry data, and present evidence in support of balloon angioplasty as a therapeutic procedure of choice for treating native and recurrent postoperative aortic coarctations. Balloon angioplasty of native aortic coarctations in 20 neonates and infants 1 year old or less reduced peak systolic pressure gradient across the coarctation from 40 +/- 12 mm Hg (mean +/- standard deviation) to 11 +/- 8 mm Hg (p less than 0.001); no patient required immediate surgical intervention. The residual gradient at follow-up (mean follow-up, 12 months) in 16 infants was 18 +/- 16 mm Hg, a significant improvement (p less than 0.01) compared with preangioplasty values. In none of the patients did an aneurysm develop. Recoarctation developed in 5 (31%) of the 16 infants and was successfully treated either by surgical resection (in 2) or by repeat balloon angioplasty (in 3). A comparison of mortality and recurrence rates between the balloon angioplasty and surgical groups was made with the help of data pooled from the literature published since 1980. The initial (7% versus 23%) and late (2% versus 25%) mortality and recoarctation (11% versus 18%) rates were higher (p less than 0.025) after surgical intervention than after balloon therapy. When only reports in which patients were operated on after 1979 were included in this type of analysis, the initial and late mortality rates remained higher (p less than 0.01) after operation than after angioplasty, and the recoarctation rates became similar (p greater than 0.1). Thirty-two children (greater than 1 year old) underwent balloon angioplasty of native coarctation with a resultant reduction in peak systolic pressure gradient from 48 +/- 19 mm Hg to 10 +/- 9 mm Hg (p less than 0.001), which continued to remain low (14 +/- 11 mm Hg; p less than 0.001) at follow-up catheterization in 24 children 13 months (mean) later. There were no immediate or late deaths. A small aneurysm developed in 1 patient (4%) but did not require intervention. Recoarctation developed in 2 patients (8%), and in both, repeat balloon angioplasty was performed with good results.(ABSTRACT TRUNCATED AT 400 WORDS)
Osteoporosis in the U. S.

Osteoporosis is an important public health problem in the US today, producing disability and even death through the development of fracture. Osteoporotic fractures are generally considered those which increase in frequency with age, occur more commonly among women and are associated with minimal trauma. These include fractures of the spine, distal forearm, hip, proximal humerus and pelvis/1/, but other fracture syndromes less well studied may also be important. It has been estimated that over 1.2 million such fractures occur each year including 500,000 vertebral frac
Introduction to PTSD Series

For many years it has been widely known that persons with severe mental illnesses such as schizophrenia, bipolar disorder, and treatment refractory depression are more likely to have suffered traum...
Spin relaxation in magnetically ordered Pd-Au (Fe 2.8 at. %) alloys

Abstract Anomalous line displacements and widths in the 57 Fe Mossbauer spectra are interpreted as spin relaxation effects and are associated with the ferromagnetic-nonferromagnetic transition in Pd-Au (Fe ≈ 2 at. %) alloys.
Reduced widths in O16 and gamma transition probabilities in F19

Reduced widths in O/sup 16/ and gamma transition probabilities in F/ sup 19/, qualitatively predicted by the cluster model, are compared with experimental data. (auth)
Prediction of Cooling of a Nocturnal Environment Using Two Atmospheric Models

Abstract A surface energy balance model and a boundary layer model were used to predict nocturnal cooling in an agricultural environment. The results from both models were compared with the observed temperatures to determine which model predicted the cooling curve more accurately. Upper air soundings and local characteristics of the nocturnal vegetated environment were processed into boundary layer model (BLM) input files. Ground station measurements were used as input to the surface energy balance model (P-model). The models were run on a real-time basis during the evening of 23 advective and radiative cooling nights. Model output was analyzed for two Florida locations, Gainesville and Ruskin. The 95% confidence intervals from t-tests, run to determine significant difference between predicted minus observed temperature and zero, averaged 3.4°C for the BLM at Gainesville, 5.8°C for the P-model at Gainesville, and 2.8°C for the BLM at Ruskin. A BLM-predicted temperature bias of + 3.4°C at the Gainesville s...
Influence of a Standard Meal on the Absorption of Controlled-Release Pseudoephedrine Capsules

The influence of a standard meal on the extent and rate of absorption of pseudoephedrine from a controlled-release (CR) capsule formulation (Pennkinetic System) was studied in 16 normal male volunteers. Equivalent single doses of an immediate-release (IR) pseudoephedrine reference syrup and the CR capsules were each studied under both fasted and postprandial conditions. Pharmacokinetic analysis evaluated the fraction of drug absorbed over time as determined by the Wagner-Nelson method. The area under the drug concentration versus time curve (AUC) results were not influenced by food or formulation, indicating that the CR formulation was absorbed to the same extent as the IR syrup. The maximum plasma concentration (Cmax) and the time to maximum concentration (tmax) tabulations under fasted conditions indicated that the CR preparation peaked at a lower level and a later time than the IR syrup. Food minimally affected the Cmax of the IR formulation, but did not affect that of the CR formulation. Food delayed the tmax of both the IR and CR preparations by < 1 h, but only the delay of the CR formulation was statistically significant. Neither delay was considered clinically meaningful. The results of this study strongly suggest that the pharmacokinetic profile of the CR pseudoephedrine capsules was minimally affected by the presence of food.
Adiabatic evolution of mass-losing stars

We have calculated the equilibrium properties of a star in a circular, equatorial orbit about a Super-Massive Black Hole (SMBH), when the star fills and overflows its Roche lobe. The mass transfer time scale is anticipated to be long compared with the dynamical time and short compared with the thermal time of the star, so that the entropy as a function of the interior mass is conserved. We have studied how the stellar entropy, pressure, radius, mean density, and orbital angular momentum vary when the star is evolved adiabatically, for a representative set of stars. We have shown that the stellar orbits change with the stellar mean density. Therefore, sun-like stars, upper main sequence stars and red giants will spiral inward and then outward with respect to the hole in this stable mass transfer process, while lower main sequence stars, brown dwarfs and white dwarfs will always spiral outward.
Handedness predicts motivation to switch from the status quo.

Degree of handedness is a correlate of structural brain asymmetries and predicts individual differences in episodic memory, belief updating and various biases in decision-making. We examined whether handedness moderated the status quo bias given previous research suggests that both constructs are related to loss aversion. Participants answered hypothetical scenarios in which they decided either to stay with the status quo or to switch. Results indicated that consistent and inconsistent right-handers both exhibited status quo bias (Experiment 1; N = 180), but inconsistent right-handers were more (or less) likely to stick with the status quo when informed of a positive (or negative) past experience. When provided with more equivocal information about the quality of the status quo and alternative, consistent-handers (CH) were more likely to show a status quo bias (Experiment 2; N = 222). Compared to CH, we argue that inconsistent-handers (IH) more readily update their beliefs in a manner consistent with how ...
Results of pancreaticoduodenectomy for lymphoplasmacytic sclerosing pancreatitis.

Objective To compare the presentation and short-term results of pancreaticoduodenectomy for lymphoplasmacytic sclerosing pancreatitis (LPSP) and pancreatic adenocarcinoma (PA) and to provide long-term follow-up on patients undergoing resection for LPSP. Background Data LPSP is a rare form of chronic pancreatitis characterized by a mixed inflammatory infiltrate centered around pancreatic ducts and ductules, combined with obliterative phlebitis. Its presentation may mimic that of PA. Methods Among 1,648 patients undergoing pancreaticoduodenectomy at the authors' institution from January 1992 to May 2002, 37 with LPSP were identified. The demographics, clinical features, and short- and long-term outcomes of these patients were analyzed. Where applicable, comparisons were made to a consecutive group of 45 patients who underwent pancreaticoduodenectomy for PA from July 2001 to December 2001. Results Twenty-four percent (9/37) of the LPSP patients and none of the PA patients had a history of either atopic or autoimmune disease. The LPSP patients had a similar clinical presentation to the PA patients, but the LPSP patients were less likely to have a discrete pancreatic mass on CT and more likely to have CT findings suggesting a diffusely enlarged pancreas. The LPSP patients were also less likely to have a discrete pancreatic mass at operation, more likely to have a diffusely firm or hard gland, and more likely to have a difficult portal vein/superior mesenteric vein dissection when compared to the PA patients. Transfusion requirement, operative time, postoperative length of stay, and overall complication rate were similar between groups; however, the LPSP patients had a greater operative blood loss. The median length of follow-up for LPSP patients was 33 months. No patients had recurrent jaundice. One patient with LPSP had clinically evident recurrent pancreatitis. Among LPSP patients available for current telephone interview, 68% subjectively rated their quality of life as better, 18% reported no change, and 14% reported diminished quality of life compared to before surgery. Conclusion LPSP mimics PA in clinical presentation, though CT findings of a diffusely enlarged pancreas without a discrete mass may suggest a diagnosis of LPSP. Nevertheless, differentiation from pancreatic neoplasia remains difficult. Patients undergoing pancreaticoduodenectomy for LPSP have durable relief of symptoms and a subjectively improved quality of life.
Roadside Restaurants and Place-Product-Packaging

With roots in the cafe, diner and tea room, various kinds of roadside restaurants evolved after 1920: roadside stands, highway coffee shops, drive-ins, outdoor walk-ups and indoor walk-ups. Together they came to represent a distinctive kind of place along the American roadside. Large corporations came to dominate highway-oriented restaurants after 1960 through “place-product-packaging”: the coordination of building design, decor, menu, service and pricing under distinctive logos. This total design in merchandising has contributed to the standardization of roadside landscapes and travel in the United States. Roadside eateries are safe, predictable oases for strangers away from home.
The Shoulder Pain and Disability Index: Is it sensitive and responsive to immediate change?

Abstract Background The Shoulder Pain and Disability Index (SPADI) is designed to detect changes in shoulder pain and disability after a one-week interval. With the new Medicare guideline, the SPADI may have to be employed for time frames of less than one week. Purpose To determine if the SPADI or its subscales could detect immediate changes in pain and function after a thoracic manipulative intervention known to produce short-term improvement and by comparing it to changes on the numeric pain rating scale (NPRS). Methods Subjects with primary complaints of non-post-surgical shoulder pain completed the NPRS and the SPADI prior to and immediately following interventions. Findings The SPADI pain subscale detected statistically significant differences that were also detected using the NPRS. In addition, the SPADI pain score and the NPRS scores were moderately correlated between the pre-intervention SPADI and NPRS scores ( r = 0.49–0.61, p r = 0.49–0.67, p Clinical relevance Since the SPADI may have to be employed in durations of less than one week secondary to third party payer requirements, it is valuable to validate the SPADI for this particular use. Conclusion Although SPADI scores demonstrated low sensitivity and responsiveness to immediate changes, the SPADI pain scale was able to detect changes in durations of less than one week. This finding should be confirmed through further prospective experimentation.
Labeling Theory and Delinquency Policy An Experimental Test

Propositions endemic to labeling theory, and variables particularly relevant to these propositions, are combined into a guiding paradigm. Components of this labeling paradigm are then tested in an ...
An operator on families of univalent functions

Abstract For f(z) = z + ∑∞n = 2 anzn analytic in the unit disk and either univalent or in a proper subclass of the family of univalent functions we investigate various relationships between f(z) and g(z) = z( f(z) z ) γ , γ real. Sufficient conditions on the coefficients of f are given for g to be starlike or convex, and coefficient bounds on g are determined.
Chorioretinal folds: associated disorders and a related maculopathy.

Purpose To describe a series of chorioretinal folds (CRFs) representing a clinical sign that may be associated with multiple systemic, orbital, and ophthalmologic disorders. We report the associations with systemic disease and describe 3 stages of a CRF-related maculopathy. Design Observational, retrospective case series. Methods We reviewed 57 affected eyes from 40 patients with the clinical sign of CRF from 1 of 2 academic institutions. A careful review of the medical histories and systemic diagnostic evaluations were conducted. Imaging studies were conducted. Results The mean age at diagnosis was 64 ± 17 years. Most eyes (n = 18) were hyperopic (+2.60 ± +2.90 diopters). There were 20 patients (50%) with some form of autoimmune disorder. Overall, the mean presenting visual acuity was 20/50, declining slightly to 20/60 over 19 ± 30 months. Ten eyes had stage 3 CRF-related maculopathy, more common in older individuals with more chronic CRFs. Four stage 3 eyes had associated choroidal neovascularization, and these eyes had 20/60 presenting visual acuity that decreased to 20/100 over approximately 1.5 years. Stage 3 eyes without choroidal neovascularization had a mean presenting visual acuity of 20/40 that decreased to 20/65 over 2.1 years. Conclusions CRFs are associated with numerous ophthalmic and systemic disorders. A careful medical history and evaluation are essential. We describe 3 stages of a unique CRF-related maculopathy. Stage 3 resembles occult choroidal neovascularization, occurs primarily in older individuals with chronic CRFs, and is accompanied by a slow deterioration in central acuity.
Promotion of sleep by gonococcal peptidoglycan fragments. Structural requirements for the somnogenic activity.

We exploited an extensive inventory of gonococcal peptidoglycan (PG) fragments to define the essential structural determinants of PG-mediated sleep-promoting activity in a rabbit sleep model. PG fragments, purified using reverse phase HPLC and structurally defined by fast atom bombardment mass-spectrometry, were administered intracerebroventricularly and the duration of specific sleep stages was determined electroencephalographically. Of the compounds tested, the principal naturally occurring sleep factor isolated from sleep-deprived animals (N-acetylglucosaminyl-[NAG]-1,6-anhydro-N-acetylmuramyl[anh.NAM]-alanyl-glutamyl-diaminopimelyl-alanine), the structurally identical PG monomer derived from gonococci, and individual analogs of the gonococcal compound which lacked the NAG residue or contained an additional alanine at the C-terminus possessed maximal potency; as little as 1 pmol of these anh.NAM-containing monomers induced excess slow wave sleep (p <.05). In fact, each of five different anh.NAM-containing disaccharide peptides tested was somnogenic at 10 pmol or less, but none of a matched set of analogous PG monomers, differing only in replacement of anh.NAM by a hydrated NAM residue, was somnogenic at this dose. Together, these data suggested that the anh.NAM end, but not the NAG moiety, is a crucial structural determinant of gonococcal PG-mediated somnogenic activity. The somnogenic activity of anh.NAM-containing fragments was also modulated (albeit to a lesser extent) by the length and composition of the peptide side chain. On a much broader basis, the data also help raise the intriguing hypothesis that bacterial products may serve as natural regulators of nervous system function in higher animals.
QoS-aware data reporting control in cluster-based wireless sensor networks

In this paper, we present an efficient data reporting control scheme in a cluster-based hierarchical wireless sensor network, which has two components: (i) intra-cluster data reporting control (IntraDRC) scheme and (ii) inter-cluster control (InterDRC) scheme. The IntraDRC scheme controls the amount of traffic generated in a cluster by selecting a certain number of data reporting nodes based on the desired throughput specified by the end system. On the other hand, the InterDRC scheme offers differentiated reporting paths from a cluster to a sink based on the traffic characteristics. InterDRC considers two parameters: one is the hop counts to a sink to deal with the end-to-end delay constraint while the other is the amount of traffic, generated in a cluster and forwarded from its adjacent clusters, to deal with energy consumption. The proposed scheme applies the block design concept from Combinatorial theory to design a novel data reporting node selection approach. IntraDRC employs the node sets created by block designs as the initial reporting schedule. This schedule can be updated by the request of a reporting node when its queue size approaches a predefined threshold. We consider two network models in the paper. The first model considers homogeneous networks in which every node has the same capabilities and adjacent cluster heads are connected in a multi-hop manner. The second model considers heterogeneous networks in which the cluster heads have high capabilities in terms of processing power and transmission range to directly reach adjacent cluster heads in a single-hop manner. Simulation results show that our scheme achieves good throughput performance while providing stable data reporting that is independent of the network density. The scheme also allows for energy savings by using load balanced data reporting paths.
Mechanical Learners Pay a Price for Bayesianism

The price is failure on a class of inductive inference problems that are easily solved, in contrast, by nonBayesian mechanical learners. By “mechanical” is meant “simulable by Turing machine”. One of the central tenets of Bayesianism, which is common to the heterogeneous collection of views which fall under this rubric, is that hypothesis change proceeds via conditionalization on accumulated evidence, the posterior probability of a given hypothesis on the evidence being computed using Bayes's theorem. We show that this strategy for hypothesis change precludes the solution of certain problems of inductive inference by mechanical means—problems which are solvable by mechanical means when the restriction to this Bayesian strategy is lifted. Our discussion proceeds as follows. After some technical preliminaries, the concept of (formal) learner is introduced along with a criterion of inferential success. Next we specify a class of inductive inference problems, and then define the notion of “Bayesian behavior” on those problems. Finally, we exhibit an inductive inference problem from the specified class such that (a) some nonmechanical Bayesian learner solves the problem, (b) some nonBayesian mechanical learner solves the problem, (c) some mechanical learner manifests Bayesian behavior on the problem, but (d) no mechanical Bayesian learner solves the problem. Insofar as possible terminology and notation are drawn from Osherson, Stob, and Weinstein [1986].
Ceftazidime Monotherapy for Empiric Treatment of Febrile Neutropenic Patients: A Metaanalysis

Whether ceftazidime monotherapy is equal in efficacy to combination regimens (Comb) for empiric treatment of febrile neutropenic patients was tested using meta-analysis. Published studies and abstracts of ceftazidime trials were identified, their quality assessed, the efficacy data abstracted and pooled, and effects of patient and study characteristics examined. The pooled odds ratio (OR) of failure of ceftazidime for febrile episodes was 1.27 (95% confidence interval [CI]: 0.79-2.03; n = 1077) and for bacteremic episodes was 0.72 (CI, 0.33-1.58; n = 248; OR less than 1.0 favors ceftazidime). Results were not significantly affected by type of antibiotic in Comb, age, neutropenia (less than 500/mm3), study quality, or combining abstracts. Results indicate that Comb does not offer a significant advantage over ceftazidime. A subgroup of profoundly neutropenic (less than 100/mm3) patients could not be assessed, raising the possibility that in this important subgroup monotherapy and Comb may not be equivalent. Use of ceftazidime empirically may require modification based on microbiologic results and clinical course.
Using checkpoints to localize the effects of faults in distributed systems

A checkpointing scheme can be used to ensure forward progress of a computation (program) even when failures occur. In a distributed system, many autonomous programs can execute concurrently and obtain services from a set of shared servers. In such a system, it is desirable to to restrict a checkpoint or rollback operation to a single program to localize the effects of failures, even when processes of different programs communicate with servers. This can be achieved by a scheme based on message logging and consistent checkpoints when the system is deterministic. When the system (communication network or programs) is nondeterministic, the semantics of the server functions should be exploited to reduce the additional synchronization that needs to be introduced to ensure locality. The authors illustrate this by presenting efficient algorithms for a file server that do not require the logging of messages on stable storage. >
Lessons from a Science Education Portal

When Cold Spring Harbor Laboratory's DNA Learning Center (DNALC) launched its Web site in 1996, [www.dnalc.org][1], we did not foresee that it would grow into a portal for 18 content sites reaching more than seven million visitors per year. The evolution of our multimedia efforts and the challenges along the way provide lessons for building learning resources or to attract larger audiences. [1]: http://www.dnalc.org
Towards force-reflecting teleoperation over the Internet

This paper extends earlier results on stable force reflecting teleoperation in the presence of significant time-delays to the case, frequent in practice, where the transmission delays are themselves varying with time in an unpredictable fashion. It shows that stability can be preserved through the systematic use of specially designed wave-variable filters. The resulting performance of the teleoperation system is illustrated in simulations, and is consistent with reasonable expectations on "ideal" behavior. The results may provide a practical tool for implementing force-reflecting teleoperation over the Internet.
Developments in Surge Research Priorities: A Systematic Review of the Literature Following the Academic Emergency Medicine Consensus Conference, 2007–2015

Objectives In 2006, Academic Emergency Medicine (AEM) published a special issue summarizing the proceedings of the AEM consensus conference on the “Science of Surge.” One major goal of the conference was to establish research priorities in the field of “disasters” surge. For this review, we wished to determine the progress toward the conference's identified research priorities: 1) defining criteria and methods for allocation of scarce resources, 2) identifying effective triage protocols, 3) determining decision-makers and means to evaluate response efficacy, 4) developing communication and information sharing strategies, and 5) identifying methods for evaluating workforce needs. Methods Specific criteria were developed in conjunction with library search experts. PubMed, Embase, Web of Science, Scopus, and the Cochrane Library databases were queried for peer-reviewed articles from 2007 to 2015 addressing scientific advances related to the above five research priorities identified by AEM consensus conference. Abstracts and foreign language articles were excluded. Only articles with quantitative data on predefined outcomes were included; consensus panel recommendations on the above priorities were also included for the purposes of this review. Included study designs were randomized controlled trials, prospective, retrospective, qualitative (consensus panel), observational, cohort, case–control, or controlled before-and-after studies. Quality assessment was performed using a standardized tool for quantitative studies. Results Of the 2,484 unique articles identified by the search strategy, 313 articles appeared to be related to disaster surge. Following detailed text review, 50 articles with quantitative data and 11 concept papers (consensus conference recommendations) addressed at least one AEM consensus conference surge research priority. Outcomes included validation of the benchmark of 500 beds/million of population for disaster surge capacity, effectiveness of simulation- and Internet-based tools for forecasting of hospital and regional demand during disasters, effectiveness of reverse triage approaches, development of new disaster surge metrics, validation of mass critical care approaches (altered standards of care), use of telemedicine, and predictions of optimal hospital staffing levels for disaster surge events. Simulation tools appeared to provide some of the highest quality research. Conclusion Disaster simulation studies have arguably revolutionized the study of disaster surge in the intervening years since the 2006 AEM Science of Surge conference, helping to validate some previously known disaster surge benchmarks and to generate new surge metrics. Use of reverse triage approaches and altered standards of care, as well as Internet-based tools such as Google Flu Trends, have also proven effective. However, there remains significant work to be done toward standardizing research methodologies and outcomes, as well as validating disaster surge metrics.
Natural and Acquired Resistance of Klebsiella-Aerobacter to Cephalothin and Cephaloridine.

SummaryOf 161 recently isolated Klebsiella-Aerobacter strains, nearly all motile strains (Aerobacter) were highly resistant to cephalothin and cephaloridine, but susceptible to tetracycline, chloramphenicol, streptomycin, kanamycin, furadantin, and the sulfonamides. In contrast, all non-motile strains (Klebsiella) were susceptible to both cephalothin and cephaloridine, more so to the former, but were frequently resistant to several of the other 6 drugs. MIC tests with Klebsiella strains frequently demonstrated inconsistent end points with one or more clear tubes occurring between tubes showing growth. This "skipped tube" phenomenon suggested rapidly acquired resistance to these cephalosporin derivatives. The rapid in vitro development of a high degree of resistance by serial transfer of the organisms in these antibiotics further suggests that clinically resistant infections by these organisms may occur with unusual frequency.
Differential expression of plasminogen activators and their inhibitors in an organotypic skin coculture system

Using immunohistochemistry and in situ hybridization, we have characterized the expression and localization of components of the plasminogen activator proteolytic cascade in an organotypic coculture system which consists of a “dermal” portion (human dermal fibroblasts throughout a collagen matrix) and a stratified, well-differentiated epidermal portion. Specifically, the following components were examined: the enzymes urokinase-type plasminogen activator and tissue-type plasminogen activator and their type 1 and type 2 inhibitors. Urokinase plasminogen activator mRNA and antigen were found predominantly in the least differentiated, basal keratinocytes; in some fields there was also faint deposition of antigen beneath the basal cells. The distribution of plasminogen activator inhibitor type 1 was similar to that of urokinase, except that inhibitor type 1 antigen deposition beneath the basal cells appeared more intense and uniform. In contrast to the results with urokinase plasminogen activator and inhibitor type 1, tissue plasminogen activator mRNA and antigen were localized focally in the suprabasal, i.e. more differentiated, keratinocytes. Plasminogen activator inhibitor type 2 mRNA and antigen were detected in most epidermal layers, but were more intense suprabasally and often spared the basal layer. These studies demonstrate that the same type of cell, i.e. the keratinocyte, can express different components of the plasminogen activator cascade depending on its state of differentiation. The change in expression of plasminogen activator cascade components with keratinocyte differentiation suggests distinct epidermal functions for these components, related to cell-matrix interaction and epidermal differentiation.
Thorax model for the studies of hemodynamic monitoring by implanted devices

Impedance incorporated implanted device provides a unique approach to monitor hemodynamics. The challenge of the use of this method is the optimization of electrode configurations. To alleviate this issue, a 3D thorax model is presented in this study. The model was developed from CT images of a patient, covering from the neck to the lower abdomen. A MATLAB -based program was developed and used to delineate different tissues/organs. The model contains 467 layers and 37 different types of tissues. Each layer had 262,144 pixels with a resolution of 1.0×1.0 mm2, approximately 122×106 pixels (voxels) in total. This high-resolution model can be used as a virtual phantom to optimize electrode configuration for the monitoring of hemodynamics by an implanted device.
Dealing with Liars: Misbehavior Identification via Rényi-Ulam Games

We address the problem of identifying misbehaving nodes that refuse to forward packets in wireless multi-hop networks. We map the process of locating the misbehaving nodes to the classic Renyi-Ulam game of 20 questions. Compared to previous methods, our mapping allows the evaluation of node behavior on a per-packet basis, without the need for energy-expensive overhearing techniques or intensive acknowledgment schemes. Furthermore, it copes with colluding adversaries that coordinate their behavioral patterns to avoid identification and frame honest nodes. We show via simulations that our algorithms reduce the communication overhead for identifying misbehaving nodes by at least one order of magnitude compared to other methods, while increasing the identification delay logarithmically with the path size.
Disruption of the Shc/Grb2 Complex during Abelson Virus Transformation Affects Proliferation, but Not Apoptosis

The v-Abl protein tyrosine kinase encoded by Abelson murine leukemia virus (Ab-MLV) induces pre-B-cell transformation. Signals emanating from the SH2 domain of the protein are required for transformation, and several proteins bind this region of v-Abl. One such protein is the adaptor molecule Shc, a protein that complexes with Grb2/Sos and facilitates Ras activation, an event associated with Ab-MLV transformation. To test the role this interaction plays in growth and survival of infected pre-B cells, dominant-negative (DN) Shc proteins were coexpressed with v-Abl and transformation was examined. Expression of DN Shc reduced Ab-MLV pre-B-cell transformation and decreased the ability of v-Abl to stimulate Ras activation and Erk phosphorylation in a Raf-dependent but Rac-independent fashion. Further analysis revealed that Shc is required for v-Abl-mediated Raf tyrosine 340 and 341 phosphorylation, an event associated with Erk phosphorylation. In contrast to effects on proliferation, survival of the cells and activation of Akt were not affected by expression of DN Shc. Together, these data reveal that v-Abl-Shc interactions are a critical part of the growth stimulatory signals delivered during transformation but that they do not affect antiapoptotic pathways. Furthermore, these data highlight a novel role for Shc in signaling from v-Abl to Raf.
CRM1-mediated Nuclear Export Is Required for 26 S Proteasome-dependent Degradation of the TRIP-Br2 Proto-oncoprotein *

Overexpression of the proto-oncogene TRIP-Br2 (SERTAD2) has been shown to induce E2F activity and promote tumorigenesis, whereas ablation of TRIP-Br2 arrests cell proliferation. Timely degradation of many cell cycle regulators is fundamental to the maintenance of proper cell cycle progression. Here we report novel mechanism(s) that govern the tight regulation of TRIP-Br2 levels during cell cycle progression. TRIP-Br2 was observed to be a short-lived protein in which the expression level peaks at the G1/S boundary. TRIP-Br2 accumulated in cells treated with 26 S proteasome inhibitors. Co-immunoprecipitation studies revealed that TRIP-Br2 forms ubiquitin conjugates. In silico analysis identified a putative leucine-rich nuclear export signal (NES) motif that overlaps with the PHD-Bromo interaction domain in the acidic C-terminal transactivation domain (TAD) of TRIP-Br2. This NES motif is highly conserved in widely divergent species and in all TRIP-Br family members. TRIP-Br2 was shown to be stabilized in G2/M phase cells through nuclear entrapment, either by deletion of the acidic C-terminal TAD, which includes the NES motif, or by leptomycin B-mediated inhibition of the CRM1-dependent nuclear export machinery. Mutation of leucine residue 238 of this NES motif abolished the interaction between CRM1 and TRIP-Br2, as well as the nuclear export of TRIP-Br2 and its subsequent 26 S proteasome-dependent degradation. These data suggest that CRM1-mediated nuclear export may be required for the proper execution of ubiquitin-proteasome-dependent degradation of TRIP-Br2.
AGE AT ONSET AS AN INDICATOR OF FAMILIAL RISK OF BREAST CANCER

The familial risk of breast cancer was investigated in a large population-based, case-control study conducted by the Centers for Disease Control. The data set included 4,730 histologically confirmed breast cancer cases aged 20-54 years and 4,688 controls who were frequency matched to cases by geographic region and 5-year categories of age. Family history of breast cancer among first-degree female relatives of cases and controls was utilized. To identify factors associated with familial risk of breast cancer, a Cox proportional hazards model was used, modeling time to onset of breast cancer among mothers and sisters. Case relatives were at greater risk than control relatives. Among relatives of cases, a significant increase in the risk of breast cancer was associated with decreasing age at onset of the case and with having an additional relative affected with breast cancer. The hazard ratio for the mother of a case with breast cancer diagnosed at 50 years of age was 1.7 (95% confidence interval (Cl) 1.4-2.0), compared with 2.7 (95% Cl 2.2-3.2) and 4.3 (95% Cl 3.3-5.6) for the mother of a case whose diagnosis occurred at 40 and 30 years of age, respectively. The hazard ratio for the sister of a case with an unaffected mother and at least one affected sister in addition to the case was 3.6 (95% Cl 2.1-6.1) when the case was diagnosed at age 50, compared with 5.8 (95% Cl 3.4-10.0) and 9.4 (95% Cl 5.3-16.7) when the case was diagnosed at 40 and 30 years of age, respectively. The hazard ratio for the sister of a case with an affected mother and no additional affected sisters was 5.9 (95% Cl 3.9-8.9) when the case was diagnosed at age 50, compared with 9.4 (95% Cl 6.2-14.4) and 15.1 (95% Cl 9.4-24.3) when the case was diagnosed at 40 and 30 years of age, respectively. The hazard ratio for the sister of a case with both an affected mother and at least one affected sister aside from the case was 17.1 (95% Cl 9.4-31.3) when the case was diagnosed at age 50, compared with 27.5 (95% Cl 15.0-50.3) and 44.2 (95% Cl 23.5-83.2) when the case was diagnosed at 40 and 30 years of age, respectively. No effect of case's menopausal status and bilaterality was found, indicating that in addition to a positive family history, age at onset is the strongest indicator of a possible genetic subtype of breast cancer in these data.
Economic policies for tobacco control in developing countries

Raising tobacco taxes can have an income distributional impact on the population. Since lower socio-economic groups usually smoke more, they also contribute more to total cigarette tax collection. Thus, those who can afford it least contribute the most in terms of tobacco taxes. This means that tobacco taxes are regressive. However, tobacco tax increases are likely to be progressive, decreasing the relative tax incidence on the poor, vis-a-vis the rich. This is based on the premise that the poor are likely to be more sensitive to price changes, and would thus reduce their cigarette consumption by a greater percentage than the rich in response to an excise tax-induced increase in cigarette prices. Recent empirical studies confirm this hypothesis by demonstrating that the price responsiveness of cigarette demand increases with income. Research in China confirmed that reducing cigarette expenditures could release household resources for spending on food, housing, and other goods that improve living standards. Therefore, in the long run, tobacco control measures will reduce social inequality.
Polarization-transparent microphotonic devices in the strong confinement limit

Microphotonic structures that strongly confine light, such as photonic crystals and micron-sized resonators, have unique characteristics that could radically advance technology1,2,3,4,5,6. However, such devices cannot be used in most applications because of their inherent polarization sensitivity; they respond differently to light polarized along different axes7,8,9. To take advantage of the distinctive properties of these structures, a general, integrated, broadband solution to their polarization sensitivity is needed. Here, we show the first demonstration of such a solution. It enables arbitrary, polarization-sensitive, strong-confinement (SC) microphotonic devices to be rendered insensitive (transparent) to the input polarization at all wavelengths of operation. To test our approach, we create the first polarization-transparent add–drop filter from polarization-sensitive microring resonators. It shows almost complete elimination of polarization sensitivity over the 60-nm bandwidth measured, while maintaining outstanding filter performance. This development is a milestone for SC microphotonics, allowing the applications of photonic-crystal and microring devices to several areas, including communications, spectroscopy and remote sensing.
Genetic spell‐checking: gene editing using single‐stranded DNA oligonucleotides

Single-stranded oligonucleotides (ssODNs) can be used to direct the exchange of a single nucleotide or the repair of a single base within the coding region of a gene in a process that is known, generically, as gene editing. These molecules are composed of either all DNA residues or a mixture of RNA and DNA bases and utilize inherent metabolic functions to execute the genetic alteration within the context of a chromosome. The mechanism of action of gene editing is now being elucidated as well as an understanding of its regulatory circuitry, work that has been particularly important in establishing a foundation for designing effective gene editing strategies in plants. Double-strand DNA breakage and the activation of the DNA damage response pathway play key roles in determining the frequency with which gene editing activity takes place. Cellular regulators respond to such damage and their action impacts the success or failure of a particular nucleotide exchange reaction. A consequence of such activation is the natural slowing of replication fork progression, which naturally creates a more open chromatin configuration, thereby increasing access of the oligonucleotide to the DNA template. Herein, how critical reaction parameters influence the effectiveness of gene editing is discussed. Functional interrelationships between DNA damage, the activation of DNA response pathways and the stalling of replication forks are presented in detail as potential targets for increasing the frequency of gene editing by ssODNs in plants and plant cells.