cssi/SciSciGPT-SciSciNet huggingface dataset:
Abstract TP142: Factors Influencing Post-stroke Discharge to Institutional Rehabilitation Settings versus Home

Introduction: We assessed relative impacts of sociodemographic, clinical, and geographic factors on discharge to rehabilitation or home in a population-based stroke study. Methods: Stroke survivors were identified from 2011-2013 in the Brain Attack Surveillance in Corpus Christi (BASIC) Project (death Results: Discharge location was available for 796/942 (85%) survivors; 15% were discharged to IRF, 26% SNF, and 59% home. Median age was 68 (IQR 58-79); 64% were Mexican American and 36% non-Hispanic white. High/low probabilities of discharge to IRF differed by 2% for sociodemographic factor variation, 7% for clinical factors, and 9% for geographic factors; probabilities of discharge to SNF differed by 22% for sociodemographic factor variation, 14% for clinical factors, and 6% for geographic factors; and probabilities of discharge home differed by 25% for sociodemographic factor variation, 24% for clinical factors, and 6% for geographic factors. Conclusion: The probability of discharge to IRF is most affected by variation in clinical factors; discharge to SNF by sociodemographic factors; and discharge home by sociodemographic and clinical factors. Geographic factors do not substantially affect discharge location.
Ulnar Collateral Ligament Injuries of the Elbow

This review discusses the current practice of evaluation and treatment of injuries to the ulnar collateral ligament (UCL) of the elbow with emphasis on rehabilitation. The latest studies revolve primarily around surgical repair and regenerative medicine for the UCL. Conservative management remains the mainstay of treatment, but surgical intervention may be advantageous in the appropriate patient. Elbow ulnar collateral ligament injuries have increased throughout the twentieth century, primarily in overhead athletes. Specifically, baseball pitchers are at high risk due to repetitive valgus elbow strain while throwing baseballs at a high velocity. Current epidemiology, anatomy, diagnosis (physical exam, imaging), and treatment (injections, surgery, and rehabilitation) of UCL injury are discussed.
Evoked potential and EEG study of the neurotoxicity of hydramethylnon in rats.

Abstract The objective of the study was to assess the neurotoxicity, using electrodiagnostic tests, of hydramethylnon (Amdro, AC 217,300), an insecticide marketed for the treatment of red imported fire ants, cockroaches, and other insects. Animals were male Fisher 344 albino rats and Long-Evans hooded rats. Brainstem auditory, visual, and somatosensory evoked potentials (BAER, VEP, SEP) and electroencephalograms (EEG) were recorded from implanted screw electrodes before and at multiple time points through day 10 after a single oral dose of hydramethylnon (at 50% or 75% of the LD50) plus vehicle, or vehicle alone. No evidence of nervous system toxicity was detected with either BAER, VEP, or SEP recordings. Spectral analysis of EEGs recorded over 7 days demonstrated a time-limited increase in power at low frequencies and decrease at high frequencies, reflecting a sedative effect. A dose-dependent weight loss was observed. Single-exposure poisonings with AC 217,300 can be expected to produce anorexia and CNS depression, but not lethality.
The deontology of a political psychoanalysis: an interview with Patricia Gherovici

Patricia Gherovici’s groundbreaking work in Lacanian psychoanalysis brings unique focus to both clinical psychology and today’s socio-political reality. In this interview, we ask Dr Gherovici to reflect on how Lacanian theory can be applied to the political issues of today. Our discussion takes place months after the Trump election and addresses such matters as unconscious choice in gender selection; rising levels of anxiety in non-cis identified analysands over Trump’s presidency; the subversive potential of the unconscious; the efficacy of grounding ethics on comedy; and employing Jacques Lacan’s notion of the sinthome to depathologize psychosis and transgender identity. Dr. Gherovici finds in her analysands a window through which to witness the impact of our social reality upon our psychic selves; and she argues for a psychoanalytic praxis that not only marries the political to the clinical, but also bases its ethics upon recognition of the singularity of the subject. In particular, she points to the subject’s individual sinthome as charting a creative response to the dire realities we each confront.
Proposal of the head-heart-base technique as an innovative method to describe and evaluate the aroma profile of wine and cider

A novel method for the sensory evaluation of wine and cider is proposed that combines advantages of other commonly used techniques while simplifying a tasting protocol that is more accessible for unexperienced panelists. The method is based on panelist’s evaluation of samples in three discrete and directed steps; evaluation of head, heart, and base characters. The technique provides the sensory analyst with a simplified approach to difference testing and, in many instances, helps statistically differentiate samples by reducing panelist confusion. The head aroma is defined as the first impression after removing the lid from a covered glass prior to agitating the sample. The heart aroma is defined as the primary impression after agitating the sample. The base character is defined as the final aroma impression and retronasal evaluation of the product. Each component is evaluated independently using a generalized grouping of descriptors; sensory impression or grouping descriptors can be refined if the analyst aims to draw a higher level of detail or descriptive analysis from their panel. Application of the proposed analytical protocol suggests that the technique is simple for less-experienced panelists to learn and follow, and allowed them to easily focus on the testing attributes with minimal confusion of confounding factors.
Conflicts Cases and the Problem of Relevant Time: A Response to the Hague Symposium

Ralph Hague, a resident of Wisconsin, was killed by an uninsured motorist in a collision in that state. Lavinia, his widow, moved to Minnesota, where she was appointed personal representative of Ralph’s estate. In that capacity Lavinia went to court in her new home state seeking a declaration that the Allstate Insurance Company owed Ralph’s estate $45,000, the aggregate of the separate $15,000 uninsured motorist coverages Ralph had bought for each of his three cars under a single insurance policy. At the time of the accident Ralph had been riding an uninsured motorcycle, and in such circumstances, under the terms of the policy, each of these coverages could be applied to the loss. Allstate defended on the ground that under the laws of Wisconsin, where the policy was issued and the insured-motorist section of the policy would limit its liability to $15,000. Minnesota applied its own law to the contrary in the widow’s favor, and, in Allstate Insurance Co. v. Hague, the United States Supreme Court eventually affirmed. Among the consequences was the Hague symposium in these pages, and the editors’ kind invitation to this writer to respond It is striking that among the symposium’s thoughtful and serious contributions to conflicts literature, none clearly takes the position that, as a matter of ordinary choice-of-law process, the choice *1024 of Minnesota law in Hague was the right one; only two are willing to state the result without criticism. And although two of these very distinguished writers conclude that the Supreme Court properly sustained Minnesota’s “parochial” choice of its own law as a matter of
High Cell-Density Fermentation

The purpose of this chapter is to educate the reader about the basic equipment and strategies used in fermentations of P. pastoris in both bench-top and pilot-scale operations. A key element in expression of foreign proteins in this yeast is the need for sufficient aeration, which is achieved by proper mixing of the media and by blending gases to control dissolved oxygen content. Automatic pH control is essential for growth and expression in P. pastoris. Finally, fed-batch fermentations require the use of peristaltic pumps and tubing capable of low rates of delivery for the feeding of nutrients and base. Teflon tubing and peristaltic pump adapters are recommended for fed-batch operations. The information in this chapter should enable a reader with little or no experience to perform a high-cell density fermentation of a P. pastoris expression strain. Although most procedures described here are specifically for the BioFlo III (NBS), it should be possible to achieve high expression levels with almost any good-quality fermentor, modified to accommodate this organism.
From Novice to expert

Publisher Summary This chapter presents a study of expert–novice differences between computer users. The results of expert–novice difference studies of computer users are consistent with corresponding research in other problem solving domains. The knowledge-based approach to assessing expert–novice differences offers a fruitful theoretical framework. The list of four types of knowledge offers a starting point, but future theoretical work is needed to specify the nature of what experts and novices know. The logical goal of this theoretical work is the development of precise models corresponding to expert and novice computer users. Research on expert–novice differences in computer users also suggests some tentative practical implications, all of which require additional study. First, to the degree that experts and novices differ with respect to the knowledge they possess, there is a role for training and education. To become an expert requires the acquisition of a body of knowledge, much of which can be taught. Second, this line of research offers some suggestions concerning how programming should be taught.
Query engine grid for executing SQL streaming process

Many enterprise applications are based on continuous analytics of data streams. Integrating data-intensive stream processing with query processing allows us to take advantage of SQL's expressive power and DBMS's data management capability. However, it also raises serious challenges in dealing with complex dataflow, applying queries to unbounded stream data, and providing highly scalable, dynamically configurable, elastic infrastructure. In this project we tackle these problems in three dimensions. First, we model the general graph-structured, continuous dataflow analytics as a SQL Streaming Process with multiple connected and stationed continuous queries. Next, we extend the query engine to support cycle-based query execution for processing unbounded stream data in bounded chunks with sound semantics. Finally, we develop the Query Engine Grid (QE-Grid) over the Distributed Caching Platforms (DCP) as a dynamically configurable elastic infrastructure for parallel and distributed execution of SQL Streaming Processes. The proposed infrastructure is preliminarily implemented using PostgreSQL engines. Our experience shows its merit in leveraging SQL and query engines to analyze real-time, graph-structured and unbounded streams. Integrating it with a commercial and proprietary MPP based database cluster is being investigated.
Celestial Architecture: Monuments of the Sky

Humans viewed the stars, imagined the structure of the universe in their star tales and cosmologies, and then very quickly began to recreate this structure on the earth in monumental buildings. These buildings took the forms of ziggurats, pyramids, cathedrals, temples, and kivas in various continents and centuries and served many purposes. One key element in making a structure part of the “celestial architecture” is to align the structure with the night sky or the morning or evening sunrise or sunset. In this chapter we explore the many ways in which humans have made “celestial architecture” that either mirrors cosmology, reflects the origins and beliefs of the people, or is aligned with morning sunrises, evening sunsets, or transits and standstills of the Sun, the Moon, and planets.
A Vocation-Based System of Ethics for Law Students

This article sets forth a set of ethical principles by which I would encourage law students to order their lives, so as to make the experience of law school, and ultimately the practice of law, more fulfilling. The principles are grounded in the concept of vocation and the idea that being a law student is one of several vocations to which individuals should be attendant. Thus, the first ethical principle is one of balance among the multiple vocations each individual must live out. The second ethical principle focuses on defining success based on faithfulness to one’s vocations.The third ethical principle deals with cooperation – appreciating that law school involves a learning community in which we can learn much, inside and outside the classroom, from our peers. The fourth ethical principle is that of pro bono service – of embracing a responsibility to use one’s talents and skills to be of service to others. Finally, the article concludes with an emphasis on the fifth ethical principle – that of reflection, of thinking about and reflecting upon how we live our lives and whether we are maintaining balance and defining success in relation to our vocations.
Ultrastructure of the eyes of the grass shrimp, Palaemonetes pugio: general morphology, and light and dark adaptation at noon

The cone cells and corneagenous cells possess extensive networks of smooth tubular endoplasmic reticulum that may be involved in optical reflectance and light-adaptational responses, respectively. The extracellular basal lamina of the basement membrane is confluent with glial cell capillary walls and may prove to be a viaduct for the transmission of hemolymph-borne substances to the retina or of retinal degradation products to the hemolymph. In addition to dense pigment granules, the distal pigment cells are shown for the first time to contain migratory reflecting platelets that are usually polymorphic in light-adapted eyes but are rectangular in dark-adapted eyes. In the latter these plates become aligned against the crystalline cones and presumably contribute to the reflection superposition optics of the grass shrimp. Dark-adapted retinular cells possess well-developed perirhabdomal cisternae, oblong or ovoid mitochondria, generally vesicular rough endoplasmic reticulum, and occasional, spherical, calcium-like intrarhabdomal inclusions. Light-adapted retinular cells possess poorly developed perirhabdomal cisternae, lamelliform rough endoplasmic reticulum, and condensed mitochondria frequently associated with lipid droplets and pigment granules. The cytoplasmic boundaries of the reflecting pigment cells expand into the extracellular spaces between individual ommatidial retinular cells during dark adaptation and recede to the interommatidial extracellular spaces during light adaptation. Cytoplasmic microfilament bundles found only at the bases of partially light-adapted rhabdomeric microvilli may be involved in microvillar shortening.
Winning at Innovation

Those who interpret innovation as invention will be losers. Those who interpret innovation as adoption of new practice in a community will be winners.
Hydrothermal liquefaction of green microalga Kirchneriella sp. under sub- and super-critical water conditions

Abstract Hydrothermal liquefaction (HTL) is one of the promising and reliable thermochemical conversion processes capable of converting wet biomass feedstock into renewable bio-oils. In this study, microalga Kirchneriella sp. was liquefied under hydrothermal conditions in a stainless-steel batch reactor. Various process parameters such as reaction temperature, pressure, biomass solid loading, and reaction duration were varied from 200 to 375 °C, 9–25 MPa, 10–20%, and 15–60 min, respectively. A one-factor-at-a-time approach was employed, and comprehensive experimental runs were further performed at 10% solid loading and a reaction time of 30 min. The maximum bio-crude yield (45.5%) was obtained at 300 °C, 9 MPa, with 10% solid loading and 30 min reaction duration. Fresh algal biomass, bio-oil and biochar samples were characterized by the ultimate and proximate analyses. The bio-oil and bio-char samples obtained at 300 °C, 9 MPa, with 10% solid loading and 30 min reaction duration have a higher heating value of 37.52 and 23.48 MJ kg−1, respectively. The HTL aqueous phase was analyzed for potential co-products by spectrophotometric techniques and is rich in soluble carbohydrates, dissolved ammoniacal nitrogen and phosphates. The metal impurities in the algae, bio-oil, and biochar were identified by ICP-OES where algae and biochar contain a large proportion of phosphorous and magnesium.
Irradiation assisted stress corrosion cracking of commercial and advanced alloys for light water reactor core internals

Abstract Thirteen alloys including high- and low-strength nickel-base alloys, austenitic stainless steels, and ferritic alloys were irradiated using 2 MeV protons to a damage level of 2.5 dpa at 360 °C and assessed for their susceptibility to irradiation assisted stress corrosion cracking (IASCC) in both BWR normal water chemistry (NWC) and PWR primary water. Cracking susceptibility was highest for high strength nickel-base alloys, followed by the low strength nickel-base alloys and then the low strength iron-base alloys. Cracking in the nickel-based alloys was worst in normal water chemistry, which was reversed for the iron-based alloys. In general, cracking correlated with the degree of microstructure changes, though no single feature could be linked to cracking. IGSCC occurred in both the unirradiated and irradiated conditions in high strength nickel-base alloys with susceptibility being considerably higher following irradiation. In all cases, slip was planar, and the degree of slip localization correlated with the probability of IG crack initiation. Low strength nickel-base alloys showed the same dependence on environment as high strength alloys but were considerably less susceptible to IASCC initiation. Among the low strength iron-base alloys, alloy 800 was most susceptible to IASCC initiation in both BWR NWC and PWR primary water, which also correlated with grain boundary chromium depletion and silicon segregation. Across all alloys, cracking correlated with both the degree of localized deformation and the hardness in the irradiated condition. The agreement is expected as increased hardening also correlates with localized deformation, which is likely a necessary, though insufficient condition for cracking.
Novel, culturally sensitive, shared medical appointment model for Hispanic pediatric type 1 diabetes patients.

BACKGROUND/OBJECTIVE Latino patients with type 1 diabetes (T1D) face cultural and language barriers leading to poor outcomes. Shared medical appointments (SMAs) are recognized as effective models of care. Our aim is to develop a culturally sensitive, cost effective SMA program for Latino T1D. SUBJECTS Spanish speaking Latinos 1 to 20 years with T1D (n = 88) and their families. METHODS Routine care alternating with SMAs that included group education was provided. Teens, ages >11 received the SMA separate from parents. Younger children were seen together. Hemoglobin A1c (HbA1c), behavioral questionnaires, and use of diabetes technology were measured at baseline and every 3 to 6 months. RESULTS 57.7% of children and 77.27% of teens completed the 2 years of the Program. There was a significant association between age and change in HbA1c from baseline to year 1 (P = .001) and baseline to year 2 (P = <.0001). For participants <12 years, there was a significant improvement in HbA1c from baseline to year 1 (P = .0146) and from year 1 to year 2 (P = .0069). Participants ≥12 years, had an increase in HbA1c from year 1 to year 2 (P = .0082). Technology use increased significantly from baseline to year 2 for participants <12 years of age (19%-60%, P = .0455) and for participants who were ≥12 years of age (10%-23%, P = .0027). Participants reported a 98% satisfaction rate. CONCLUSIONS The culturally sensitive SMA proved to be an appreciated, feasible, and effective alternative to care for Latinos with T1D.
Illinois Civil Procedure: Chapter 1

The book, published annually, covers civil practices in the Illinois state courts. It primarily focuses on trial procedures in the Illinois Circuit Courts. The book is available both in print and electronic form. The book chiefly reviews both the language of and cases on pertinent Illinois Supreme Court Rules and Illinois Civil Procedure Code provisions, both general and special. It also reviews some local circuit court rules, as well as case precedents untethered to any written laws and civil procedure statutes outside the Code (which often deal with the special processes for certain types of claims, so-called statutory proceedings, like marriage dissolution and probate). While the book is presented as a comprehensive lawyer’s manual, it is often used in law school courses on Illinois civil procedure, frequently supplemented by related contemporary skills training/cutting edge problems (many are available from the author). The Table of Contents for the book is attached. It contains 31 chapters as well as an appendix containing annotated law review/law journal cites to materials relevant to each chapter. Also attached is a draft of Chapter 1 which covers the history of the Illinois Court system and its civil procedure lawmakers, reprinted from Illinois Civil Procedure, 2018-2019 edition with permission; copyright 2018 Matthew Bender & Co., a Lexis Nexis company; all rights reserved.
A Historical Pragmatic Study of Apologies: A Case Study of the Essex Pauper Letters (1731-1837)

In this paper, the realizations of apologies as evidenced in the Essex pauper letters of 19th century England are explored. The paper takes a critical look at the forms and functions of apologies, arguing that apologies in such texts are conventionalized in form. Taking into consideration the social norms of writing specific to this speech community, the study makes a distinction between two main functions of apologies and argues that apologies under scrutiny are not a politeness device that repairs and redresses an offence; rather, they exemplify a politic behavior that helps in the negotiation of interpersonal relationships and the attainment of the writers’ discursive goal.
Guanine, pyrazolo[3,4-d]pyrimidine, and triazolo[4,5-d]pyrimidine (8-azaguanine) phosphonate acyclic derivatives as inhibitors of purine nucleoside phosphorylase.

Phosphonate acyclic derivatives of guanines, pyrazolo[3,4-d]pyrimidines, and triazolo[4,5-d]pyrimidines (8-azaguanines) are inhibitors of the enzyme purine nucleoside phosphorylase (PNPase) with Ki‘ values ranging from 0.05 to 1.6 μM. These compounds are enzymatically stable congeners of the potent PNPase inhibitor acyclovir diphosphate (53).
Abstract 2233: Correlation between mutations found in FFPE tumor tissue and paired cfDNA samples

Cell free DNA (cfDNA) consists of small (150 - 500 bp) DNA fragments that circulate in the blood. cfDNA levels tend to be low in healthy, non-pregnant patients, and increase in patients with cancer, pregnancy, or extensive damage to tissue. cfDNA is believe to derive mostly from apoptotic cells, and biomarkers for a variety of diseases have been found in cfDNA. As cfDNA is extracted from blood, it is a non-invasive way to detect disease; however, there is some concern that cfDNA does not contain the same biomarkers as tumor tissue. This study measures the efficacy of cfDNA as a biomarker detection medium by comparing mutations found in both FFPE tumor samples and paired cfDNA samples. This study determines whether the same biomarkers are found in each sample type, and which of those can be used as biomarkers in both and which are preferred biomarkers for only a single sample type. Trends in mutation detection with the two different sample types are discussed. Citation Format: Lauren Saunders, Antonia Hur, Brittany Niccum, Asmita Patel. Correlation between mutations found in FFPE tumor tissue and paired cfDNA samples [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 2233.
Estimation of the Effect of OAT2-Mediated Active Uptake on Meloxicam Exposure in the Human Liver

Active uptake mediated by organic anion transporter 2 (OAT2) has been previously hypothesized as a key player in hepatic disposition of its substrates. Previous studies have shown that another hepatic uptake transporter, organic anion transporting polypeptides (OATP) 1B1, significantly elevates liver concentrations of drugs transported by it. As tissue concentration typically governs pharmacodynamics, drug-drug interactions, and toxicity in the liver, it is important to understand if OAT2 functions similarly to OATP1B1 in raising liver exposure. Since this is a research problem that cannot be easily assessed in clinical studies at this time, here we estimated human liver exposure of an OAT2 substrate meloxicam using a deduction method based on physiologically based pharmacokinetic (PBPK) modeling of clinical systemic exposure data. Although in vitro data suggest that OAT2-mediated active uptake is involved in meloxicam disposition, the modeling result concludes that its unbound liver exposure is unlikely significantly different from its unbound systemic exposure. This conclusion is further supported by data and modeling from a terminal monkey study and in vitro hepatocyte studies with bovine serum albumin. Overall, based on currently available data, we do not expect that OAT2 has a strong impact on the liver exposure of meloxicam.
Outcomes of Pediatric Syndactyly Repair Using Synthetic Dermal Substitute.

Purpose Full-thickness skin grafts are classically used for areas of skin deficit in syndactyly reconstruction surgery. However, skin grafting requires additional time under anesthesia and includes donor site morbidity. Synthetic dermal substitute has produced favorable web creep and scar appearance outcomes in syndactyly reconstruction. We sought to validate these results using objective outcome measures. Methods All patients who had undergone syndactyly reconstruction with synthetic dermal substitute with 1-year follow-up were identified. We included 23 webs in 16 patients; 2 were lost to follow-up. Median age at surgery was 27 months; 7 patients were female. Five webs were revision surgeries. Two patients had a diagnosis of amniotic constriction band, and 3 had symbrachydactyly. All webs were assessed from blinded clinical photos. Outcome measures included web creep assessment (range, 0–5) and Vancouver Scar Scale. Results Of 21 webs, 2 had minimal creep and 16 had none; 3 had clinically relevant creep (grade 2). Vancouver Scar Scale scores for all webs averaged 1.19 (0 is normal). There was normal vascularity in 20 webs, pigmentation was normal in 17 webs, skin pliability was normal in 13 webs, and scar height was flat in 15 webs. On the visual analog scale assessment, surgeons rated the appearance of the 21 webs as an average of 8.8 (range, 5–10). There were no postoperative complications. Conclusions Synthetic dermal substitute is an effective, efficient, and visually satisfactory option for coverage of skin deficits in syndactyly reconstruction. The current heterogeneous sample revealed that this approach can provide satisfactory outcomes for patients, families, and surgeons. Although no surgical complications were noted, the rate of integration and healing has yet to be determined. Type of study/level of evidence Therapeutic IV.
Drug-Induced Lupus, a One-time Hit or a Harbinger of Future Autoimmunity: A Case Report.

Introduction Drug-induced lupus (DIL) can comprise up to 10% of new lupus cases annually, and the list of medications associated with DIL is increasing. However, it can be difficult to recognize the connection between symptoms and a medication-induced autoimmune syndrome, which can lead to an invasive, costly workup. Given that the prognosis is usually good if therapy with the offending agent is stopped, it is important to identify this clinical entity promptly. Case presentation A healthy, 44-year-old man with hypertension was seen initially because of shoulder pain and again after development of fevers and chest pain. He underwent a thorough infectious workup and then oncologic workup, with his clinical course complicated by a Histoplasma infection. After evaluation by subspecialists, the patient was thought to have an autoimmune condition related to DIL. His symptoms improved after he discontinued the offending drug therapy and received a course of corticosteroids. Discussion Our case highlights how DIL should be on the differential when seemingly disparate symptoms develop in a patient receiving DIL-associated medications. Lupus is one of the "great imitators," in which symptoms can be ascribed to many different underlying causes. Although this patient's presentation may have been confounded by concomitant histoplasmosis, his improvement with cessation of hydralazine treatment argues in favor of DIL. His continued atypical serologic test results could be residual from his DIL and should normalize with time. However, it raises the question whether this bout of DIL has unmasked a previously quiescent autoimmune condition, requiring continued observation.
Characterization of sequence specific binding of LARP6 to the 5’ stem-loop of type I collagen mRNAs and implications for rational design of antifibrotic drugs

Abstract Excessive synthesis of type I collagen characterizes fibrotic diseases. Binding of LARP6 to the 5’ stem-loop (5’SL) of collagen mRNAs regulates their translation and the high rate of biosynthesis in fibrosis. LARP6 needs two domains to form stable complex with 5’SL RNA, the La-domain and the juxtaposed RRM domain (jointly called the La-module). We describe that the La-domain of LARP6 is necessary and sufficient for recognition of 5’SL in sequence specific manner. The three amino acid motif, RNK, located in the flexible loop which connects the second α-helix to the β-sheet of the La domain is critical for binding. Mutation of any of these three amino acids abolishes the binding of La-domain to 5’SL. The major site of crosslinking of LARP6 to 5’SL RNA was mapped to this motif. The RNK motif is not found in other LARPs, which can not bind 5’SL. Presence of RRM increases the stability of complex between La-domain and 5’SL RNA and RRM domain does not make extensive contacts with 5’SL RNA. We propose a model in which the initial recognition of 5’SL by LARP6 is mediated by the RNK epitope and further stabilized by the RRM domain. This discovery suggests that the interaction between LARP6 and collagen mRNAs can be blocked by small molecules that target the RNK epitope and will help rational design of the LARP6 binding inhibitors as specific antifibrotic drugs.
Abstract 17326: Blockade of Thrombospondin1 Attenuates Tgfβ Signaling and Enhances the Automaticity of Tbx18-Induced Pacemaker Cells

Introduction: Transcription factor Tbx18 can reprogram chamber cardiomyocytes to induced pacemaker cells (iPMs). Transcriptomic analysis of Tbx18-iPMs identified Tgfβ pathway as a prominent signali...
From Being System-Involved to Changing the System: Infiltrating the System:

One model for engaging young people to be more civically minded and to help them develop a critically conscious sociopolitical identity is through youth organizing and participatory action research...
Grandma's Garden Seeds

Arthritis pinched her flesh and pricked her nerves; it jerked her knees with cold hands till the foundation of the house trembled to her heavy breathing. As far back as her mind stretched, there wasn't a time since she lived here and her knees didn't swell or hurt deeply. Grandma mixed hot water and Epsom salt to soak her stiffness and wipe away the pain. In gray hours of low clouds she felt like chopping off the knees and throwing them in the ocean, with all the throbbing and burning they gave. It didn't feel natural to be living in such a cold place, but she had been living here so long she'd be a stranger any where else.
Progestin receptors from tissues either exhibiting or lacking estrogen response mechanisms. Comparison of conventional and high-performance liquid chromatography methodology.

Abstract Evidence from a variety of target organs has shown that progesterone receptor (PR) is induced by estrogen receptor (ER) in normal and neoplastic tissues. However, approximately 12% of the normal human uterine samples exhibit only PR with no measurable ER, suggesting the expression of both inducible and constitutive receptor isoforms. We investigated several molecular properties of PR from tissues either exhibiting or lacking ER. All studies were conducted in potassium phosphate buffer containing 10 m M sodium molybdate with a synthetic progestin, [ 3 H]R5020 as the ligand. Radioinert R5020 was used as competitor to assess nonspecific association. Competition analysis showed that PR from both sources exhibited similar ligand specificities and affinities. Relative affinities were ORG 2058 > R5020 > medroxyprogesterone acetate > progesterone /2>-testosterone K d values ranged from 10 −9 to 10 −10 M ; testosterone showed no specific competition). We utilized high-performance liquid chromatography in the size-exclusion (HPSEC) and ion-exchange (HPIC) modes to probe the size and ionic properties of PR. HPSEC profiles showed that the PR isoform from both sources was eluted as a single, sharp peak > 76 A. HPIEC elution profiles indicated no differences in the surface ionic properties in that PR from both tissue types eluted with ca . 100 m M phosphate. These experiments show no difference between the inducible and the putative constitutive form of PR. Thus, some PR species may not require estrogen for their formation.
The clinical utility of vocal dosimetry for assessing voice rest

Objectives/Hypothesis Voice rest is frequently recommended following surgical disruption of vocal fold epithelium, but patients report variable adherence to voice rest recommendations. The objective of this study was to assess the clinical utility of an ambulatory vocal dosimeter for measuring adherence to voice rest recommendations. Study Design Outcomes research. Methods Part 1: To determine the utility of the dosimeter in nonclinical use, the relationship between self-reported voice use and dosimeter measurements was examined in normal subjects (n = 11) who prospectively logged voice use while wearing the dosimeter. Part 2: To determine clinical utility of the dosimeter, patients undergoing vocal fold surgery for which postoperative voice rest was recommended (n = 11) wore a dosimeter for 2 days prior to and 2 days after surgery. Phonation percent and sound level were compared at baseline and during voice rest. Results The dosimeter performed as hypothesized with both normal subjects and patients. A moderate correlation (r = 0.62) was noted between self-reported voice use and dosimeter measurements in normal subjects. In patients on voice rest, a statistically and clinically significant decrease was observed in measured voice use, both in phonation time (P = .002) and intensity of phonation (P = .004). Conclusions Ambulatory vocal dosimetry may have clinical utility for assessing adherence to voice rest recommendations. This information will be useful for the design of future studies on voice rest. Level of Evidence 2c Laryngoscope, 125:171–176, 2015
A practical synopsis of consumption coagulopathy.

Abstract : The objective of this report is to develop a practical synopsis of consumption coagulopathy. Current methodology to recognize and diagnose consumption coagulopathy is reviewed. A consumption coagulopathy panel is devised as a data source for improved methodology. A panel of very simple coagulation tests was devised which can be readily repeated, day or night, weekday or weekend, on demand. It is designated 'consumption coagulopathy panel' to serve as a source of data for diagnosis, surveillance, and therapeutic decisions con erning the case at hand. The report provides a table listing the parameters of the standard consumption coagulopathy panel and the qualitative changes which occur with each parameter for all three types. In addition, the recommended treatment is correlated with the type of consumption coagulopathy. (Author)
DESIGNING PRODUCTS AND PROCESSES FOR POSTPONEMENT

Companies that compete in a global market have to manufacture and configure products to meet the diverse needs and demands of customers. Such diverse needs can result from geographical differences, government regulations, or simply differences in customer preferences and tastes. Uncertainty in the market place makes it extremely difficult to forecast product demands accurately. This results in high inventory costs and low product availability. Postponement is a strategy whereby the final configuration of a product is delayed as much as possible, usually until a customer order is received. Postponement ranges from simple packaging and labeling, bulk packing or special customization, to more complex forms such as localization, assembly and test, and final product integration. Effective implementation of postponement requires careful redesign of the products and the processes involved. In this paper, we describe a conceptual framework for design for postponement, and the appropriate cost drivers that can be used to assess postponement strategies for a company.
Nematogenic fluids in shear flow: A laboratory for nonequilibrium physics

We have briefly described some of the issues, techniques and anticipated phenomenology associated with the modification of the equilibrium isotropic-nematic transition in thermotropic liquid crystals due to applied shear stress. Whilst having significant implications in its own right — from the physics nonequilibrium phase transitions in complex fluids to the processing of modern materials — this subject also appears to provide a rich and fascinating environment in which to explore fundamental issues of nonequilibrium physics.
Template-Based Structured Argumentation

A semiautomated approach to evidential reasoning uses template-based structured argumentation. A template captures best analytic practice as a hierarchically structured set of coordinated questions; an argument answers the questions posed by a template, including references to the source material used as evidence to support those answers. Graphical depictions of arguments readily convey lines of reasoning, from evidence through to conclusions, making it easy to compare and contrast alternative lines of reasoning. Collaborative analysis is supported via simultaneous access to arguments through web browser clients connected to a common argument server. This approach to analysis has been applied to a wide range of analytic problems and has been experimentally shown to speed the development and improve the quality of analytic assessments.
Balancing clarity and politeness in editing sessions with non-native speakers

When communicating with writers who are non-native speakers, technical editors need to be both clear (to prevent miscommunication) and polite (to maintain good working relationships). Concepts from speech act theory can be used to identify linguistic forms that are maximally clear, as well as those that are maximally polite. A problem arises, however, because these two sets of forms do not entirely overlap: maximally clear forms such as direct speech acts may be perceived as impolite, while maximally polite forms such as indirect speech acts may be ambiguous and therefore not entirely clear. Research on intercultural pragmatics can be used to decide how to choose or modify specific linguistic forms so as to maintain both clarity and politeness when communicating with non-native speakers.
The Professionalization of Baccalaureate-Level Social Work

We are occasionally privileged to observe an important event in the history of some aspect of life, but one is rarely an eyewitness to the birth of a profession—or even a specific division of a profession. Nevertheless, along with others, I had the privilege of participating in a series of events over several years that, with hindsight, I realize made me a witness to the birth of baccalaureate social work. This article reflects the perspective of one person engaged in these events and may differ from the observations of other participants. Yet, it represents my best recollection of events that began nearly fifty years ago.
T.S. Eliot’s Theory of Culture

This essay was supposed to be included in CP II but unaccountably omitted. It contains unique and substantial clarifications of the concepts of social class and culture, concepts curiously not focused upon so extensively elsewhere in the oeuvre. There are also indications that Schutz had been studying cultural anthropology. He regarded Eliot highly as a poet but not as a social theorist.
Determination of N-retinylidene-N-retinylethanolamine (A2E) levels in central and peripheral areas of human retinal pigment epithelium

The bis-retinoid N-retinylidene-N-retinylethanolamine (A2E) is one of the major components of lipofuscin, a fluorescent material that accumulates with age in the lysosomes of the retinal pigment epithelium (RPE) of the human eye. Lipofuscin, as well as A2E, exhibit a range of cytotoxic properties, which are thought to contribute to the pathogenesis of degenerative diseases of the retina such as Age-related Macular Degeneration. Consistent with such a pathogenic role, high levels of lipofuscin fluorescence are found in the central area of the human RPE, and decline toward the periphery. Recent reports have however suggested a surprising incongruence between the distributions of lipofuscin and A2E in the human RPE, with A2E levels being lowest in the central area and increasing toward the periphery. To appraise such a possibility, we have quantified the levels of A2E in the central and peripheral RPE areas of 10 eyes from 6 human donors (ages 75–91 years) with HPLC and UV/VIS spectroscopy. The levels of A2E in the central area were on average 3–6 times lower than in peripheral areas of the same eye. Furthermore, continuous accumulation of selected ions (CASI) imaging mass spectrometry showed the presence of A2E in the central RPE, and at lower intensities than in the periphery. We have therefore corroborated that in human RPE the levels of A2E are lower in the central area compared to the periphery. We conclude that the levels of A2E cannot by themselves provide an explanation for the higher lipofuscin fluorescence found in the central area of the human RPE.
Slipped capital femoral epiphysis : a physeal lesion diagnosed by MRI, with radiographic and CT correlation

Objective. To define and compare early lesions associated with slipped capital femoral epiphysis (SCFE) on magnetic resonance imaging (MRI), computed tomography (CT) and radiography. Design and patients. Thirteen patients with 15 symptomatic hips due to SCFE underwent radiography and MRI; CT was performed in 12 patients. SCFE was graded on radiographs, head/neck angles and qualitative changes were evaluated on CT, and morphologic/signal abnormalities were determined on MRI. Results. Physeal widening, apparent on T1-weighted MRI, was evident in every case of SCFE, including one presumed “pre-slip.” T2-weighted images demonstrated synovitis and marrow edema but obscured physeal abnormalities. CT head/neck angles ranged from 4–57° for symptomatic to 0–14° for asymptomatic hips. Physeal and metaphyseal changes were variably identified on both radiographs and CT in all cases of SCFE, but not in the pre-slip. Conclusion. MRI clearly delineates physeal changes of both pre-slip and SCFE, and demonstrates very early changes at a time when radiographs and CT may appear normal.
Serial Bilirubin Determinations as a Prognostic Marker in Clinical Infections

Patients with documented serious infection and total bilirubin values of >2 mg/dl were surveyed for serial changes in bilirubin and other laboratory and clinical features. Of 19 patients studied, 12 (Group A) had persisting or increasing hyperbilirubinemia, and 7 (Group B) had decline in bilirubin after infection onset. None demonstrated marked changes in other liver tests. Only one patient had infection directly involving the hepatobiliary system. There were no significant differences between the two groups with respect to underlying illnesses, active hepatobiliary diseases, pathogens, bacteremia, or administration of cholestatic drugs. All Group A patients died because of uncontrolled infections, whereas all Group B patients survived with resolution of infection (p < .001). Ten of 15 patients with available preinfection liver tests demonstrated serial bilirubin increases without marked changes in other liver tests prior to clinical recognition of infection. These findings demonstrate that hyperbilirubinemia disproportionate to increases in other tests may manifest before recognition of infection and that persistent or progressive hyperbilirubinemia is indicative of ongoing active infection.
Adenylate kinase 3 sensitizes cells to cigarette smoke condensate vapor induced cisplatin resistance.

Background The major established etiologic risk factor for bladder cancer is cigarette smoking and one of the major antineoplastic agents used for the treatment of advanced bladder cancer is cisplatin. A number of reports have suggested that cancer patients who smoke while receiving treatment have lower rates of response and decreased efficacy of cancer therapies. Methodology/Principal Findings In this study, we investigated the effect of cigarette smoke condensate (CSC) vapor on cisplatin toxicity in urothelial cell lines SV-HUC-1 and SCaBER cells. We showed that chronic exposure to CSC vapor induced cisplatin resistance in both cell lines. In addition, we found that the expression of mitochondrial-resident protein adenylate kinase-3 (AK3) is decreased by CSC vapor. We further observed that chronic CSC vapor-exposed cells displayed decreased cellular sensitivity to cisplatin, decreased mitochondrial membrane potential (ΔΨm) and increased basal cellular ROS levels compared to unexposed cells. Re-expression of AK3 in CSC vapor-exposed cells restored cellular sensitivity to cisplatin. Finally, CSC vapor increased the growth of the tumors and also curtail the response of tumor cells to cisplatin chemotherapy in vivo. Conclusions/Significance The current study provides evidence that chronic CSC vapor exposure affects AK3 expression and renders the cells resistant to cisplatin.
Immediate placement of intrauterine devices after first and second trimester pregnancy termination.

Abstract Background We reviewed our experience with intrauterine device (IUD) placement after surgical abortion up to 20 weeks' gestation. Study Design Women presenting for elective abortion between January 2004 and March 2009 who requested an IUD were included in this retrospective review. Results Of 308 women requesting postabortion IUD placement, 221 (72%) planned insertion at the time of abortion (immediate group) and 87 (28%) planned insertion at their postoperative visit (interval group). IUDs were placed in 96% of the immediate group and in 23% of the interval group (212/221 vs. 20/87; p Conclusion Immediate postabortion IUD insertion is safe and effective. Given the low rate of return for interval insertion, immediate placement may be preferable.