cssi/SciSciGPT-SciSciNet huggingface dataset:
Fatal systemic inflammatory response syndrome in a ornithine transcarbamylase deficient patient following adenoviral gene transfer

Abstract We report the death of an 18-year-old male with partial ornithine transcarbmaylase (OTC) deficiency who participated in a pilot (safety) study of gene therapy. The vector used for this trial was based on human adenovirus type 5, deleted in E1 and E4, and contained human OTC cDNA. It was infused into the right hepatic artery at a dose of 6 × 10 11 particles/kg. Approximately 18 h. following gene transfer the subject was noted to have altered mental status and jaundice—clinical signs not seen in any of the first 17 subjects in this study. Subsequently, his clinical course was marked by systemic inflammatory response syndrome, biochemically detectable disseminated intravascular coagulation, and multiple organ system failure, leading to death 98 h following gene transfer. Post-mortem examination was consistent with the clinical course, and vector DNA sequences were readily detectable in most tissues. The subject had high serum levels of IL-6 and IL-10 but normal TNFα immediately after infusion of the vector. This experience points to the limitations of animal studies in predicting human responses, the steep toxicity curve for replication defective adenovirus vectors, substantial subject-to-subject variation in host responses to systemically administered vectors, and the need for further study of the immune response to these vectors.
You can see what 'I' means

This paper takes up the question of whether we can visually represent something as having semantic value. Something has semantic value if it represents some property, thing or concept. An argument is offered that we can represent semantic value based on a variety of number-color synesthesia. This argument is shown to withstand several objections that can be lodged against the popular arguments from phenomenal contrast and from the mundane example of reading.
Motor improvement with bilateral subthalamic nucleus deep brain stimulation in a patient with levodopa-responsive secondary parkinsonism

Abstract At many centers, subthalamic nucleus (STN) deep brain stimulation (DBS) is not considered for patients with secondary forms of parkinsonism, due to higher expected rates of treatment failure. We present the case of a woman with secondary parkinsonism that developed following a Measles, Mumps, and Rubella (MMR) vaccination, who experienced sustained improvement in motor function following STN DBS. Despite the diagnosis of a secondary parkinsonism, this patient responded well to dopaminergic therapy, a good predictor of DBS outcome in patients with idiopathic Parkinson’s disease. This case suggests that DBS may be considered in the setting of secondary parkinsonism if such patients have levodopa-responsive symptoms.
Role of Workforce Management in Bridge Superstructure Labor Productivity

There have been many studies on different aspects of the construction process in regard to how they each impact construction productivity. In reviewing the documentation of this research, very few ...
Southern provenance of upper Jackfork Sandstone, southern Ouachita Mountains: Cathodoluminescence petrology

The source urea of the upper Jackfork Sandstone of the southern Ouachita Mountains of Arkansas has been located by means of Cathodoluminescence (CL) of quartz in conjunction with standard petrography. Comparison of frequency distributions of quartz CL colors between the Jackfork and approximately coeval sandstones of the Black Warrior basin detected genetically based affinities which were not apparent by inspection of gross petrology. The upper Jackfork was derived from the same source area as the Parkwood Formation of the Black Warrior basin of Alabama. The Parkwood is known to have come from the south, and so the Jackfork did also. The Jackfork represents a deep-water lithologic equivalent of the deltaic and nearshore marine Parkwood, deposited as turbidites in the deeper water of the Ouachita trough.
High-Conductance Conformers in Histograms of Single-Molecule Current−Voltage Characteristics

Understanding charge transport across single molecular junctions is essential for the rational design and optimization of molecular device components. However, the correlation between calculated and experimental transport properties of single molecules probed by current–voltage (I–V) characteristics is often uncertain. Part of the challenge is that molecular conductance is sensitive to several factors that are difficult to control, including molecular orientation, conformation, aggregation, and chemical stability. Other challenges include the limitations of computational methodologies. Here, we implement the Σ-Extended Huckel (EH) nonequilibrium Green’s function (NEGF) method to analyze the histogram of I–V curves of 4,4′-diaminostilbene probed by break-junction experiments. We elucidate the nature of the molecular conformations with a widespread distribution of I–V curves, typically probed under experimental conditions. We find maximum conductance for molecules that are not at the minimum energy configur...
Anatomy of the Amazon Gold Rush

Anatomy of the Amazon Gold Rush. David Cleary. Iowa City: University of Iowa Press, 1990. xvi. 245 pp., illustrations, maps, tables, glossaries, index. $17.95 (paper). ISBN 0-87745-262-8.
Associations of retrospective and concurrent lipid levels with subclinical atherosclerosis prediction after 20 years of follow-up: the Coronary Artery Risk Development in Young Adults (CARDIA) study

Abstract Purpose Using data from the Coronary Artery Risk Development in Young Adults (CARDIA) study, we sought to determine how well lipids measured at baseline and at 20 years predict the presence of subclinical atherosclerosis. Methods Complete risk factor, coronary artery calcification (CAC), and carotid intima media thickness (CIMT) data were available for 2435 participants. Lipids were categorized into quartiles, CAC at Y20 was dichotomized as present/absent, and CIMT was dichotomized as ≥84 or Results Lipid levels measured in young adulthood as well as middle age were both associated with subclinical disease in middle age. The discriminatory value of lipids was virtually identical at baseline, when participants were 18–30 years of age, and 20 years later. Neither baseline nor Y20 lipid data were strong predictors of Y20 subclinical disease despite statistically significant associations. Conclusions These results are consistent with a growing body of evidence that early-life exposure to nonoptimal lipids matters and lifestyle modifications administered earlier in the lifespan could slow the progress of the atherosclerotic plaques.
THE PREPARATION AND PROPERTIES OF MACROPHAGE-L CELL HYBRIDS

The plasma membrane of the mouse peritoneal macrophage has specific receptors which enable the cell to bind IgG or complement-coated sheep red cells and is also rich in a divalent cation-dependent adenosine triphosphatase (ATPase) activity. L cells lack these macrophage membrane markers. The question of macrophage membrane receptor expression was investigated in DBA/2 mouse macrophage x mouse LMTK- cell hybrids produced with the aid of Sendai virus. Three independent clones and one mass culture were isolated by their ability to grow in hypoxanthine, aminopterin, and thymidine (HAT) selection medium. These hybrids retained 85–100% of the sum of two parent cells' chromosomes and expressed several genes derived from both parents, including glucose phosphate isomerase isozymes and H-2 antigens. The hybrids displayed ATPase activity which was intermediate between that of the macrophage and L cell. The macrophage specific receptors for antibody or complement-coated red cells could not be demonstrated on hybrid cells. The selective absence of these receptors is probably because of a failure in gene expression rather than to loss of genes.
Patterns of Turbulent and Double-Diffusive Phenomena: Observations from a Rapid-Profiling Microconductivity Probe

Abstract Throughout much of the ocean interior, the diapycnal buoyancy flux is maintained by both mechanical and double-diffusive processes. Assessing the relative roles of each is a challenge, particularly in complex coastal environments. During February–March 1995, a repeat-profiling CTD system, equipped with a dual-needle microconductivity probe, was deployed off the central California coast (35°N, 121°W) from the research platform FLIP. The probe’s vertical resolution (8 cm) appears sufficient to resolve the low wavenumbers of the turbulent inertial subrange. This paper presents depth–time maps, spanning 12 days and 100–400 m, of temperature dissipation rate χ, and Cox number Ĉ. High χ and Ĉ values tend to occur in layers, on a variety of spatial scales. Simultaneously, finescale (6.4-m) Richardson number, effective strain rate, and Turner angle are measured. The occurrence of intense microstructure fluctuations is correlated with all three quantities, affirming that both mechanical turbulence and d...
Development of a Sensitive Bead-Based Assay for Enhanced Monoclonal Antibody Detection

Monoclonal antibodies are increasingly used in the treatment of cancer due to their enhanced targeting and immune system stimulation properties. Dosage guidelines typically do not account for personal cancer load or metabolism, thereby possibly affecting treatment outcome or causing unwanted side effects. The requirement for an assay that can quickly and precisely measure the concentration of the monoclonal antibody in a serum sample of a patient during therapy is unmet. A bead-based assay with peptide antigen mimetics has been developed to rapidly determine the concentration of antibody drug present in serum specimens with high sensitivity. Alemtuzumab (anti-CD52) and rituximab (anti-CD20) antigen mimetic peptides, as discovered by phage display, were synthesized on 10 um TentaGel resin beads using conventional solid phase peptide synthesis techniques. The beads were modified to allow for multiplexing and microfluidic handling via fluorescent labeling and magnetic functionalization. The antigen-displaying fluoromagnetic particles were incubated with spiked serum samples which allowed free antibody to be captured. Primary antibody detection was performed on alemtuzumab while rituximab detection was used to compensate for non-specific serum binding to the beads. After washing, the beads were incubated with a fluorescently tagged secondary label for detection by flow cytometry. (Results) A fast, low cost, specific assay has been developed with several key techniques which allows detection at low concentration (0.1ug/ml) of spiked samples. Primary to achieving this detection limit was the implementation of a compensation scheme where two antigen mimetic peptides behave linearly (R2=0.996) which enables the calculation of the zero response of the antigen mimetic peptide of interest (alemtuzumab antigen mimetic) while measuring the zero response of the compensatory antigen mimetic peptide (rituximab antigen mimetic) during primary assay measurement. This reduces fluorescence response variation due to variations present due to sample preparation, storage and different patients because of the equivalent interactions these effects have on the compensatory beads. The developed assay is therefore robust against serum variation and enables a lower limit of detection.
Hardware libraries: An architecture for economic acceleration in soft multi-core environments

In single processor architectures, computationally- intensive functions are typically accelerated using hardware accelerators, which exploit the concurrency in the function code to achieve a significant speedup over software. The increased design constraints from power density and signal delay have shifted processor architectures in general towards multi-core designs. The migration to multi-core designs introduces the possibility of sharing hardware accelerators between cores. In this paper, we propose the concept of a hardware library, which is a pool of accelerated functions that are accessible by multiple cores. We find that sharing provides significant reductions in the area, logic usage and leakage power required for hardware acceleration. Contention for these units may exist in certain cases; however, the savings in terms of chip area are more appealing to many applications, particularly the embedded domain. We study the performance implications for our proposal using various multi-core arrangements, with actual implementations in FPGA fabrics. FPGAs are particularly appealing due to their cost effectiveness and the attained area savings enable designers to easily add functionality without significant chip revision. Our results show that is possible to save up to 37% of a chip's available logic and interconnect resources at a negligible impact (< 3%) to the performance.
The open verifier framework for foundational verifiers

We present the Open Verifier approach for verifying untrusted code using customized verifiers. This approach can be viewed as an instance of foundational proof-carrying code where an untrusted program can be checked using the verifier most natural for it instead of using a single generic type system. In this paper we focus on a specialized architecture designed to reduce the burden of expressing both type-based and Hoare-style verifiers.A new verifier is created by providing an untrusted executable extension module, which can incorporate directly pre-existing non-foundational verifiers based on dataflow analysis or type checking. The extensions control virtually all aspects of the verification by carrying on a dialogue with the Open Verifier using a language designed both to correspond closely to common verification actions and to carry simple adeQuacy proofs for those actions.We describe the design of the trusted core of the Open Verifier, along with our experience implementing proof-carrying code, typed assembly language, and dataflow or abstract interpretation based verifiers in this unified setting.
Effect of administration of caffeine or green tea on the mutation profile in the p53 gene in early mutant p53-positive patches of epidermal cells induced by chronic UVB-irradiation of hairless SKH-1 mice

Irradiation of SKH-1 mice with UVB light for 20 weeks resulted in a large number of patches of epidermal cells, which was visualized with an antibody that recognizes mutated p53 protein. Oral treatment of mice with caffeine (0.4 mg/ml) or green tea (6 mg tea solids/ml) as the drinking fluid during UVB irradiation decreased the number of patches by approximately 40%. Sequencing analysis of the p53 gene (exons 3 to 9) detected 88, 82 or 39 point mutations in 67, 70 or 29 patches from water, caffeine or tea treated mice, respectively. A major hotspot at codon 270 (Arg-->Cys) accounted for 47.7% (water), 70.7% (caffeine) or 46.2% (tea) of all mutations. Patches from caffeine treated mice had fewer types of mutations than patches from mice treated with water or tea. Administration of caffeine or tea during 20 weeks of UVB irradiation eliminated mutations at codons 149 (Pro-->Ser) and 210 (Arg-->Cys) but increased the frequency of mutations at codon 238 (Ser-->Phe). Topical applications of caffeine (1.2 mg in 100 microl acetone) once a day, five times a week for 6 weeks after stopping UVB decreased the number of patches by 63% when compared with mice treated with acetone. DNA sequencing analysis detected 63 and 68 mutations in 48 and 57 patches from acetone or caffeine treated mice, respectively. Although no differences in the frequency, position or types of mutations were observed, the caffeine group harbored less homozygous mutations (12.3% of the total) than the acetone group (31.3% of the total, P = 0.029). In summary, oral treatment of mice with caffeine or green tea during chronic UVB irradiation changed the mutation profile of the p53 gene in early mutant p53 positive epidermal patches, and topical applications of caffeine after discontinuation of chronic UVB irradiation specifically eliminated patches harboring homozygous p53 mutations.
Pelvic Endometriosis: Spectrum of Magnetic Resonance Imaging Findings

Endometriosis is defined as the presence of endometrial tissue outside the uterus. It is a common cause of pelvic pain and infertility among reproductive age women. Although laparoscopy remains the reference standard for diagnosis, this invasive procedure provides little information on subperitoneal extent of disease or areas hidden by adhesions. In contrast, magnetic resonance imaging (MRI) provides a comprehensive, noninvasive survey of the pelvis with simultaneous tissue characterization. In this article, we review the spectrum of MRI findings in pelvic endometriosis and discuss common complications associated with the disease. Radiologists should be familiar with the spectrum of MRI findings of pelvic endometriosis so that they can provide meaningful information that will guide treatment and future laparoscopic examinations.
Modeling the Western Arctic Caribou Herd During a Positive Growth Phase: Potential Effects of Wolves and Radiocollars

Abstract Population modeling exercises can lead to both expected and unexpected results useful for wildlife research and management, even though inferences must often be qualitative, given underlying assumptions. Our main objective was to use empirical data on wolf (Canis lupus) kill rates and growth of the Western Arctic caribou (Rangifer tarandus) herd (WAH) of Alaska, USA, to assess the potential for predator regulation. We used available data and published literature to construct a deterministic density-dependent population model fitted to trends of the WAH from 1976 through 2003. By increasing wolf densities in the baseline model, we failed to reject the hypothesis that wolves at a density of 6.5 wolves per 1,000 km2 could regulate a caribou herd at a density of 0.4 caribou per km2. In addition, our model may be conservative by underestimating the regulatory potential of wolves. We suggest that this relatively simple predator–prey system shows signs of a predation–food 2-state model. Elasticities fro...
Automated high speed stitching of large 3D microscopic images

High-resolution microscopic imaging of biological samples often produces multiple 3D image tiles to cover a large field of view of specimen. Usually each tile has a large size, in the range of hundreds of megabytes to several gigabytes. For many of our image data sets, existing software tools are often unable to stitch those 3D tiles into a panoramic view, thus impede further data analysis. We propose a simple, but accurate, robust, and automatic method to stitch a group of image tiles without a priori adjacency information of them. We first use a multiscale strategy to register a pair of 3D image tiles rapidly, achieving about 8∼10 times faster speed and 10 times less memory requirement compared to previous methods. Then we design a minimum-spanning-tree based method to determine the optimal adjacency of tiles. We have successfully stitched large image stacks of model animals including C. elegans, fruit fly, dragonfly, and mouse, which could not be stitched by several existing methods.
A survey on the controllability of fuzzy logic systems

This theoretical investigation is targeted at seeking a controllability index or condition for fuzzy logic systems, similar to the one for linear time-invariant (LTI) systems in modern control theory. Such an index should be specified in terms of systems' key fuzzy quantities such as for example, the systems' fuzzy relational matrices and the like. >
PATCH CHOICE AND FORAGING BEHAVIOR OF NONBREEDING AMERICAN FLAMINGOS IN YUCATÁN, MEXICO

Abstract We studied nonbreeding American Flamingos (Phoenicopterus ruber ruber) using the Celestun Lagoon, Yucatan, Mexico, to characterize feeding patches and to describe factors influencing patch choice. Four invertebrate taxa (polychaetes, molluscs, chironomids, crustaceans) and two plant foods (seeds of widgeongrass [Ruppia maritima] and tubercles of muskgrass [Chara fibrosa]) comprised 99% of potential food items in 76 patches we sampled. Patches tended to be dominated either by invertebrates or plant foods; only 12% of patches had 50:50 ratios of plant and invertebrate foods. Food was more abundant in invertebrate versus plant-dominated patches (157 vs. 67 items patch−1), but flamingo flock size was smaller in invertebrate-dominated patches (60 vs. 147 birds patch−1). More individuals walk-fed as invertebrates increased in the patch, and more birds stamp-fed as plant foods increased. We argue that groups of foraging flamingos apparently indicate location of food for conspecifics (local enhancement),...
Biological Energy Recovery Using Dairy Cow Waste

Laboratory research which applied anaerobic digestion to dairy manure was concerned with optimizing gas production for potential energy recovery. The digesters were operated successfully at loading rates of 0.14 lb VS/cu ft/day to 0.56 lb VS/cu ft/day (2.3 kg/m³/day to 9.2 kg/m³/day) which corresponded to SRT values of 7.5 to 30 days. Calculated heat balances for a 1,270-cu ft (36 m³) digester indicate that winter is the critical time for which the system must be economical. Operation at a high loading rate and short SRT are recommended for maximizing energy recovery. Estimated energy surpluses for a 60-cow herd increased from 116,000 Btu/day to 722,000 Btu/day (35,000 kg.cal/day to 199,000 kg.cal/day) for the loading rates examined. Operation of the prototype digester is being directed by the laboratory findings. The prototype digester, including primary and secondary zones, has a fluid volume of 3,600 cu ft (100 m³).
AN EIGENVALUE METHOD FOR THE CALCULATION OF NUCLEAR BURNUP

An iterative approach to the reactor burnup problem is developed on the basis of analytical solutions for the variable-coefficient burnup equations. The time dependence of the depletion matrices, A(t), is approximated by a polynomial representation. The number of basic time points for which spatial-diffusion calculations during burnup are required is determined only by the order of approximation necessary to give a reasonably good fit for the time dependence of A(t). Usually a low-order approximation is sufficient, so the number of diffusion calculations is reduced to a minimum. The method is applicable both to survey-type calculations and to detailed reactor-burnup studies. A comparison of some results obtained with the method described and with standard calculational methods is given. The results show the rapid convergence and accuracy of the proposed procedure. (auth)
Snooping and Sexting: Digital Media as a Context for Dating Aggression and Abuse Among College Students

Digital dating abuse (DDA) is a pattern of behaviors that control, pressure, or threaten a dating partner using a cell phone or the Internet. A survey of 365 college students was conducted, finding that digital monitoring behaviors were especially common. There were no gender differences in number of DDA behaviors experienced, but women reported more negative hypothetical reactions to sexual messaging than men. DDA was associated with measures of physical, sexual, and psychological dating violence. Results suggest that digital media are a context for potentially harmful dating behaviors, and the experience of DDA may differ by gender for sexual behaviors.
Crystal structures of fac-tri-chlorido-tris-(tri-methyl-phosphane-κP)rhodium(III) monohydrate and fac-tri-chlorido-tris-(tri-methyl-phosphane-κP)rhodium(III) methanol hemisolvate: rhodium structures that are isotypic with their iridium analogs.

The crystal structures of two solvates of fac-tri­chlorido­tris­(tri­methyl­phosphane-κP)rhodium(III) are reported, i.e. one with water in the crystal lattice, fac-[RhCl3(Me3P)3]·H2O, and one with methanol in the crystal lattice, fac-[RhCl3(Me3P)3]·0.5CH3OH. These rhodium compounds exhibit distorted octahedral coordination spheres at the metal and are isotypic with the analogous iridium compounds previously reported by us [Merola et al. (2013). Polyhedron, 54, 67–73]. Comparison is made between the rhodium and iridium compounds, highlighting their isostructural relationships.
Next-Generation Applications to Translation Research

ps3 The rapid escalation of throughput from next generation sequencing instruments, coupled with decreasing costs per experiment, is resulting in a rapid accumulation of knowledge about the genome-wide spectrum of variation in disease. One result of such discoveries will be the correlation of certain variants with disease susceptibility, outcome, and other clinical disease aspects. Variants with correlative significance have translational potential, and must be coupled with an appropriate technology that has a straightforward template preparation method, hands-off operation, and a read-out that can be subjected to algorithmic interpretation. This talk will focus on the translational potential that may result from our work to sequence cancer genomes using whole genome methods and extensive analytical characterization.
Urinary incontinence and estrogen.

? -yrinajry incontinence I is an important issue I for peri-and post I menopausal women. ^k??S In a recent study of U.S. women, half of the partici? pants between the ages of 50 and 90 experienced urine leak? age every month.1 And the sever? ity of incontinence was found to increase with age. Prompt and effective treatment of urinary incontinence symptoms is possi? ble but requires an open conver? sation in which the nurse takes
Raman Spectroscopic Studies of the Vulcanization of Rubbers. III. Studies of Vulcanization Systems Based on 2-Mercaptobenzothiazole

Abstract The shoulder observed at approximately 440 cm−1 in Raman spectra of CB vulcanizates prepared from MBT based vulcanizing systems has been shown to consist of two components. There are Raman lines contributing at 440 cm−1 and 424 cm−1. The former is due to ZnO present as an extra-network material while the latter appears to be associated with polysulfidic structures. The Raman line at 505 cm−1 seen in extracted vulcanizates prepared from CB-MBT-Sulfur-ZnO-Lauric acid recipes does not appear to be solely due to disulfidic structures. The major contribution appears to be associated with an unsaturated cyclic sulfide and is most probably due to a structure of the type (I). If the assignment to cyclic sulfidic structures such as (I) is confirmed, it will have major significance with regard to the mechanism of accelerated sulfur vulcanization.
Synthesis of Granular Systems by Cluster Beam Deposition

Granular films were prepared by simultaneous cluster beam and physical vapor deposition. The clusters are formed by high pressure sputtering and have an average size between 2 and 5 nm. This method makes it possible to control the granule size and concentration, which is not possible using precipitation synthesis of granular alloys. Most importantly, a diverse set of granule/matrix systems are accessible by this system. The method is illustrated for systems of interest for giant magneto-resistance applications: Co/Cu (miscible), and Co/Cr (immiscible.) Synthesis of films with isolated embedded clusters of more complex structures, e.g. intermetallics Ni 3 Al and Cu 3 Au, are also discussed.
Abstract 5208: Overexpression of EZH2 increases multipotent mammary stem cell self-renewal and differentiation

Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL BACKGROUND: A role for EZH2 in stem cell maintenance was recently reported. Our lab has previously demonstrated that overexpression of EZH2 accelerates the initiation of ErbB2-induced mammary tumors. To understand underlying mechanism, we investigated the role of EZH2 in regulation of stem cells and luminal progenitor cells utilizing xenotransplantation studies of preneoplastic mammary glands of EZH2+; neu and EZH2 wt; neu transgenic mice. METHODS: Generation of crossing EZH2+; neu and EZH2 wt; neu transgenic mice was described previously. Mouse handling and experimental procedures were conducted in accordance with the NIH Guide for the Care and Use of Laboratory Animals and were approved by the IACUC of the UM. Stem cells (Lin-/CD49f+/ESA-) and luminal progenitor cells (Lin-/CD49f+/ESA+) were isolated from the hyperplastic mammary glands of 8 weeks old EZH2+; neu and EZH2 wt; neu mice by flow cytometry (n=15 per group). Stem cells and luminal progenitor cells were injected into cleared fat pads of 3 weeks old FVB mice. After 5 weeks the mammary glands were subjected to flow cytometry and histology. RESULTS: Histologic study showed that only the stem cells, but not the luminal progenitor cells, extracted from EZH2+; neu and EZH2 wt; neu transgenic mice were able to form mammary outgrowths. Outgrowths of EZH2+;neu mice had significantly increased epithelial proliferation and increased numbers of terminal end buds (p<0.05) compared to EZH2 wt;neu outgrowths. EZH2 +;neu outgrowths had significant upregulation of Ki67 and pSTAT5 proteins. Flow cytometry of the outgrowths revealed that while all cell populations were increased, EZH2 induced a more pronounced expansion of the stem cell and luminal progenitor cell populations. CONCLUSIONS: Our data show that EZH2 overexpression in ErbB2 mice induces an expansion of the multipotent mammary stem cell population with the ability to regenerate the cellular heterogeneity of the mammary gland and develop hyperplastic outgrowths. We found that EZH2 promotes hyperproliferation and upregulation of STAT5 phosphorylation in the mammary epithelium in vivo. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 5208. doi:1538-7445.AM2012-5208
Demand Expansion and Cannibalization Effects From Retail Store Entry: A Structural Analysis of Multi-Channel Demand

In this paper, we propose a structural framework to study multi-channel demand. Our model explains a comprehensive set of demand outcomes as a function of prices and retail store proximity, including the frequency with which consumers shop, how much they spend per purchase occasion, whether they buy from the online (web) or retail channel, and how they allocate expenditures among multiple product categories. We allow channels to convey different amounts of information about product categories, which in turn affects a consumer's expected utility from purchasing in a particular channel. For example, physical inspection of goods in the retail channel can provide information about product fit and feel that is difficult to assess in the online channel. Another distinguishing feature of our model is that shopping trip expenditures are endogenously determined in the first phase of a multi-stage budgeting process, where consumers allocate their income by trading off utility for the outside option and the expected utility from optimal channel and category expenditure choices with the focal brand. A key methodological contribution of the paper is to advance a highly efficient algorithm to compute optimal expenditures, which facilitates joint estimation of the model parameters by maximum simulated likelihood.We estimate the model using the purchase histories of approximately 10,000 randomly selected customers from a firm that uses both online and retail channels to sell directly to consumers. The firm doubled its retail footprint over our two year observation window, providing a rich source of customer-specific variation in retail store proximity that we leverage to identify the demand effects of interest. We find evidence of channel complementarity through increased overall shopping frequency as the distance to retail outlets decreases, accompanied by increased substitution from online to retail formats. Our estimates imply a 10% reduction in retail store distance increases existing customer annual revenues by 0.53%, by increasing retail revenues 1.96% and decreasing online revenues by 1.43%. In a series of counterfactual experiments, we demonstrate how our model can be used as a decision tool for managers to identify promising locations for new physical stores and to explore channel-based price discrimination policies.
Philosophical Concepts in Physics: The scientific enterprise

I hold that in all cases of inductive inference we must invent hypotheses, until we fall upon some hypothesis which yields deductive results in accordance with experience. Such accordance renders the chosen hypothesis more or less probable, and we may then deduce, with some degree of likelihood, the nature of our future experience, on the assumption that no arbitrary change takes place in the conditions of nature. William Jevons, The Principles of Science: A Treatise on Logic and Scientific Method Pure logical thinking cannot yield us any knowledge of the empirical world; all knowledge of reality starts from experience and ends in it. Propositions arrived at by purely logical means are completely empty as regards reality. Because Galileo saw this, and particularly because he drummed it into the scientific world, he is the father of modern physics – indeed, of modern science altogether. A complete system of theoretical physics is made up of concepts, fundamental laws which are supposed to be valid for those concepts and conclusions to be reached by logical deduction. It is these conclusions which must correspond with our separate experiences. The structure of the system is the work of reason; the empirical contents and their mutual relations must find their representation in the conclusions of the theory. In the possibility of such a representation lie the sole value and justification of the whole system, and especially of the concepts and fundamental principles which underlie i t … [T] hese latter are free inventions of the human intellect, which cannot be justified… a priori .
Quantitative leak test for microholes and microtears in whole gloves and glove pieces

Abstract The current leak tests for gloves are qualitative. The developed quantitative leak test uses vacuum pressure to draw measured volumes of water to detect microholes/tears in whole gloves and glove pieces. A modified plastic vacuum desiccator interfaced with a Frazier air permeability tester allowed exposure of disposable unsupported/unlined/powderless Kimtech Blue nitrile to 50 mL of water for glove pieces or to 600 mL within a whole glove at vacua of 8–9 in. (20–23 cm) and 11–12 in. (28–30 cm) water gauge, respectively. Punctures of known dimensions were made before testing in specific glove areas using 21-, 22-, 26-, 30-, and 33-gauge needles (outer/inner diameters in micrometres of 873/514, 794/413, 635/311, 476/127, 318/159 and 238/133, respectively). The length of the punctures varied from 0.13 ± 0.01 to 0.80 ± 0.11 mm. Flow rates of water through the holes/tears ranged from 2.5 ± 0.4 to 106 ± 7 mL/min for glove pieces. For whole gloves, the ranges were from 31 ± 9 to 543 ± 110 mL/min in the palm area; and 0.23 ± 0.06 to 82 ± 18 mL/min in the finger/fingertip area. The method quantified tear lengths as short as 0.13 ± 0.01 mm.
Linear Time Average Consensus on Fixed Graphs

Abstract We describe a protocol for the average consensus problem on any fixed undirected graph whose convergence time scales linearly in the total number nodes n. More precisely we provide a protocol which results in each node having a value within an e of the initial average after O Inln l|x ‘ x l|2 ) iterations. The protocol is completely distributed, with the exception of requiring all nodes to know the same upper bound U on the total number of nodes which is correct within a constant multiplicative factor.
Arterial elasticity in testicular cancer survivors.

9604 Background: Testicular cancer survivors are known to have an increased risk of cardiovascular (CV) disease. We aimed to explore the impact of platinum based chemotherapy on endothelial functio...
Toll-like receptor 8 agonist R-848 and IFN-γ synergize for IL-12 production by myeloid DC through expressions of Interferon Consensus Sequence Binding Protein (IRF-8) and Interferon Regulatory Factor-1 (IRF-1)

6001 Toll-like receptor (TLR) 8 agonist R-848 and IFN-γ have been shown by us to prime myeloid derived dendritic cells (DC) for high level IL-12 production that induces a Th1 cellular immune response and CD8+ T cells displaying high functional avidity against tumor cells. It would be crucial for cancer vaccine development to determine whether IFN-γ and TLR-8 agonist R-848 can cooperate in the induction of IL-12 and the pathways that mediate IL-12 production. By priming human monocyte-derived DC with the TLR-8 agonist R-848 and IFN-γ for 4 hours followed by CD40L maturation, we observed that IL-12 secretion from DC was 5-20 fold greater compared with that seen using either R-848 or IFN-γ alone. Kinetic studies showed that this synergy resulted not only in the enhanced level of IL-12 production, but also in prolonged secretion time. DC primed by R-848 alone no longer responded to furtherCD40L stimulation if the maturation signal was provided beyond 8h of R-848 treatment. However, addition of IFN-γ to R-848 treatment enabled DC to produce large amounts of IL-12 when CD40 was engaged 24h after R-848 and IFN-γ treatment. Northern Blot analysis revealed that R-848 primarily up-regulated IRF-8 mRNA expression in DC and IFN-γ was a main inducer of IRF-1. Western Blot confirmed that IRF-8 and IRF-1 proteins were increased in such DC. IRF-8 and IRF-1, both transcription factors of the IFN Regulatory Factor Family, have been reported to synergistically activate IL-12p35and p40 gene transcriptions and induce IL-12p70 secretion. Our studies indicate that R-848 and IFN-γ synergistically prime DC for high level of IL-12 production possibly through IRF-1 and IRF-8. These results provide a mechanism to enhance the effectiveness of cancer vaccine taking advantage of the IL-12 inducing properties of the IFN-γ and R-848.
Simulations of the surfaces of soda lime aluminoborosilicate glasses exposed to water

Molecular dynamics simulations of seven compositionally different sodium calcium alumino-borosilicate glasses showed formation of 4B and 5Al more consistent with experimental data without compromising the other structural features that match experimental results observed in recent simulations of these glasses. Analysis of the dry surfaces of these glasses show a lack of 4B in the top 5-6A of the surface in comparison to the bulk concentration for all glasses and no 5Al. Upon exposure to water, the simulations show that the 3B in the top 5-6A of the glasses are preferentially attacked, decreasing the number of B bonds to O originally from the glass, indicating a change in the glass network. Inclusion of all B-O bonds in the top 5-6A (i.e. including O from water) shows a decrease in 3B but an increase in 4B that is consistent with NEXAFS analysis, which the simulations show are hydroxylated. There is an increase in the concentration of 3Al in the dry surface in comparison to the bulk, but exposure to water converts almost all of these 3Al to 4Al. Hydroxyl concentrations vary from 2.6/nm2 to 4.1/nm2, with SiOH and BOH dominating these surface hydroxyls. Upon exposure to water, network linkages to B are preferentially ruptured. This, and the preferential loss of the non-bridging oxygen sites attached to Na, provide atomistic evidence of the initial stages of removal of B and Na from glass surfaces exposed to water. This article is protected by copyright. All rights reserved.
Relationship between loneliness and symptoms of anxiety and depression in African American men and women: Evidence for gender as a moderator

Abstract We examined for gender differences in loneliness and negative affective conditions, namely, symptoms of anxiety and depression, in 168 African American college students. Gender differences were examined as a predictor of negative affective conditions and as a moderator of the relationship between loneliness and negative affective conditions. Although no significant differences were found in levels of loneliness and negative affective conditions between African American men and women, results of regression analyses indicated that gender differences significantly predicted anxious and depressive symptoms. Moreover, a significant Gender × Loneliness interaction effect was found in predicting both anxious and depressive symptoms. Specifically, higher levels of anxious and depressive symptoms were found among lonely African American women, compared to men. These findings suggest that the negative psychological effects of loneliness may be particularly more damaging for African American women than men. Understanding the unique challenges of African American women, compared to men, as a double minority is discussed.
Nonresponse bias in survey research: lessons from a prospective study of breast reconstruction

Abstract Background Survey-based research is essential for evaluating the outcomes of health care in an era of patient-centered care. However, many such studies are hampered by poor response rates in completion of study questionnaires, thus limiting the generalizability of any findings. The objectives of this analysis were to identify independent variables associated with nonresponse to surveys following breast reconstruction to improve future patient-reported outcomes research. Materials and methods The Mastectomy Reconstruction Outcomes Consortium is a prospective cohort study involving 11 leading medical centers from the United States and Canada. Nonresponse rates for surveys assessing satisfaction with breast, satisfaction with care (BREAST-Q), depression (Patient Health Questionnaire-9), and anxiety (Generalized Anxiety Disorder-7) were measured at 1 y and 2 y postoperatively. Clinical complication rates were compared between responders and nonresponders, and multivariable models were used to assess predictors of nonresponse. Results Among 2856 women in the analytic cohort, 1882 (65.9%) underwent implant-based, 817 (28.6%) received autologous, and 157 (5.5%) underwent latissimus dorsi myocutaneous flap breast reconstructions. Nonresponse rates to surveys at 1 y and 2 y were 27.8% and 34.4%, respectively. Race, ethnicity, and annual household income were associated with nonresponse to surveys. Women who underwent implant-based procedures were less likely to complete long-term surveys. Conclusions As survey-based research plays an increasingly prominent role in evaluating the outcomes of breast reconstruction, we found socioeconomic and procedure-related differences in survey response rates. Investigators must consider systematic differences in response rates among particular groups of women on the generalizability and validity of findings and perform rigorous nonresponse bias analyses.
The Times, They Need A Changing: Infusing Social Justice Into Kinesiology Requires Collaboration

ABSTRACTWe live in challenging times. The divisions among people related to race, gender, gender identity, religion, and socioeconomic class are becoming more apparent. Past efforts toward social justice and the dismantling of these divisions seem to be losing their momentum and are even negated. As kinesiologists, we each need to commit to standing for social justice with every opportunity we are presented with and most importantly, in our classes. After a review of terms and clarification of ideas related to diversity and social justice, this lecture will present the position that collaboration will provide an effective approach to develop the knowledge, skills, and confidence essential to appropriately address diversity in our professional lives and advocate for social justice. This collaboration should be across disciplines and move us beyond our own research agendas to working with those whose research agendas or areas of expertise are diversity and social justice. Our collective commitment and colla...
Donald Trump and the Politics of Lying

Donald Trump is probably the biggest liar in the history of the modern US presidency. He tells repeated Big Lies despite empirical evidence and well-documented media reports contracting his lies. When confronted with contrary evidence, Trump and his handlers dismiss any critical claims about Trump as “fake news” and “alternative facts”. Echoing Chairman Mao and Comrade Stalin, Trump calls the media “the enemy of the people” and rarely does a day go by without a barrage of attacks and rants on his Twitter account. Trump is also the biggest bullshitter to inhabit the modern presidency, constantly bragging about himself and his magnificent accomplishment, never failing to BS to the nth about his amazing achievement and fabulous presidency. Thus, the Trump Regime can be seen as “post-truth” and hyper-Orwellian in its use of blatant lies, propaganda, and pure bullshit.
Ozone Indicators Determined at Rural Sites in the Eastern United States by two Monitoring Networks

Ozone levels at Clean Air Status andTrends Network (CASTNet) and nearby rurally-designatedState and Local Air Monitoring Stations (SLAMS) siteslocated in the eastern United States (U.S.) arecompared using daily indicators (i.e., maximum, range,and SUM06) based on hourly O3 measurements. Comparisons are also made using monthly and summertimethree-month SUM06 determinations aggregated from dailydata. Comparison of O3 indicators at the pairedCASTNet-SLAMS sites generally shows better agreementfor O3 maximum than for range or SUM06. Thehighest correlation of daily O3 indicators occursfor paired sites that are separated by less than 100miles, and the correlation diminishes with increasingseparation distance. Correlation coefficients exceed0.70 in 49% of the comparisons of maximum, in23% of range comparisons and in 5% ofSUM06 comparisons, suggesting that substitution ofSLAMS for CASTNet O3 measurements would fail tocapture a substantial amount of the variability inO3 indicators present at the CASTNet sites. Correlations of O3 indicators at CASTNet andpaired nearby SLAMS sites are ≥0.80 in a relativelysmall number of cases. Despite the high correlationin even these cases, O3 behavior at paired sitesis not identical and shows systematic differences thatare reflected in the O3 minimum, hourly averageconcentrations, and the monthly and three-month SUM06. Ozone measurements at nearby rurally-designated SLAMSsites may not capture the `rural' nature of the airquality being monitored at CASTNet sites and, in mostcases, may not be sufficiently representative of theconcentration at CASTNet sites to permit O3measurements at SLAMS sites to be used in lieu ofmeasurements at CASTNet sites. As a result, if ruralO3 concentration data are needed, then O3monitoring at carefully chosen, rurally representativesites is recommended.