crossing96/oncoscreening huggingface dataset:
Risk of cancer among firefighters: a quantitative review of selected malignancies.	Using the fixed-effect model, the author quantitatively estimated the risks of cancers of the colon, bladder, kidneys, and brain as well as non-Hodgkin's lymphoma and leukemia among firefighters. The risk of these six cancers was not markedly elevated when cohort mortality studies were considered. When all mortality studies were considered, however, there was a mild increase in risk for kidney cancer and non-Hodgkin's lymphoma, with a summary relative risk (sumRR) of 1.22 (95% confidence interval [CI] = 1.02-1.43) and 1.40 (95% CI = 1.20-1.60), respectively. A subcohort analysis based on duration of employment revealed that firefighters with 30 or more years of employment had a significantly increased mortality risk for colon cancer, sumRR of 1.51 (95% CI = 1.05-2.11); kidney cancer, sumRR of 6.25 (95% CI = 1.70-16.00); brain cancer, sumRR of 2.53 (95% CI = 1.27 7.07); and leukemia, sumRR of 2.87 (95% CI = 1.43-5.14). After firefighters had 40 or more years of employment, their risk of mortality was significantly increased for colon cancer, sumRR of 4.71 (95% CI = 2.03-9.27); kidney cancer, sumRR of 36.12 (95% CI = 4.03-120.42); and bladder cancer, sumRR of 5.7 (95% CI = 1.56-14.63). The risk for non-Hodgkin's lymphoma was elevated but not significantly so among firefighters with 20 or more years of employment, with sumRR of 1.72 (95% CI = 0.90-3.31). Kidney cancer risk was significantly elevated as early as the second decade of employment.	bladder
Bladder cancer in Taiwan: relationship to trihalomethane concentrations present in drinking-water supplies.	The objective of this study was to evaluate whether exposure to disinfection by-products (DBP) is associated with bladder cancer. A matched case-control study was used to investigate the relationship between the risk of death from bladder cancer and exposure to total trihalomethanes (TTHM) in drinking water in 65 municipalities in Taiwan. All bladder cancer deaths of the 65 municipalities from 1996 through 2005 were obtained from the Bureau of Vital Statistics of the Taiwan Provincial Department of Health. Controls were deaths from other causes and were pair-matched to the cases by gender, year of birth,and year of death. Each matched control was selected randomly from the set of possible controls for each cancer case. Data on TTHM levels in drinking water in study municipalities were collected from the Taiwan Environmental Protection Administration. The municipality of residence for cancer cases and controls was assumed to be the source of the subject's TTHM exposure via drinking water. The adjusted odds ratios for bladder cancer death for those with high TTHM concentrations in their drinking water were 1.8 (1.18-2.74) and 2.11 (1.43-3.11) compared to the lowest group. The results of this study show that there was a significant positive correlation between the concentration of TTHM in drinking water and risk of death from bladder cancer.	bladder
Microsatellite abnormalities and somatic down-regulation of mismatch repair characterize nodular-trabecular muscle-invasive urothelial carcinoma of the bladder.	AIMS: To correlate histological infiltration patterns with genetic and mismatch repair (MMR) profiles in muscle-invasive bladder urothelial carcinomas (UroC). METHODS AND RESULTS: Infiltration patterns were assessed in the deep compartment of muscle-invasive UroC (nodular-trabecular, 45 cases; infiltrative, 27 cases). Tumour compartment (superficial and deep to muscularis mucosa) analysis included: microsatellite pattern of TP53, RB1, WT1 and NF1 by polymerase chain reaction/denaturing gradient gel electrophoresis; mitotic, Ki67, in situ end labelling (ISEL) indices and DNA ploidy. MMR was assessed by MLH1 and MSH2 sequencing and immunohistochemistry in UroC with two or more abnormal microsatellite loci. Statistical differences were tested using anova and Fisher's exact tests. Infiltrative UroC showed lower Ki67 index 14.94 +/- 4.28, ISEL index 14.1 +/- 10.0 and shorter median survival (20 months) than nodular-trabecular UroC (Ki67 index 20.65 +/- 4.94, ISEL 20.2 +/- 22.7, 37-month survival, respectively). The genetic profile was significantly different for RB1 (P = 0.0003) and NF1 (P = 0.0023) only, being more frequently abnormal in nodular-trabecular UroC. A significant decrease in MLH1 or MSH2 protein expression with no gene mutations was identified in UroC with microsatellite abnormalities and a nodular-trabecular growth pattern. CONCLUSIONS: Somatic down-regulation of MMR proteins in nodular-trabecular muscle-invasive UroC results in RB1/NF1 microsatellite abnormalities, correlating with higher cellular turnover and longer survival.	bladder
Effects of N-methyl pyrrolidone on the uptake of hypericin in human bladder carcinoma and co-staining with DAPI investigated by confocal microscopy.	Photodynamic diagnosis (PDD) using hypericin (HY), a natural photosensitizer, detects bladder cancer significantly better than white light endoscopy. However, the lipophilicity of HY complicates its administration for clinical applications. Currently, pharmaceutical preparations for HY without plasma protein are being developed. Formulations containing a biocompatible solvent, N-methyl pyrrolidone (NMP) have been shown to enhance the photodynamic therapeutic effects of HY. It was recently reported that, NMP formulations of HY were able to produce significantly higher contrast for fluorescence detection of tumors than albumin-containing HY formulations. This present work hypothesizes that NMP acts both as a solvent and penetration enhancer to improve the delivery of HY into cells by increasing the permeability of cell membranes. This paper reports the use of 3-D confocal microscopy to monitor real-time uptake of HY in human carcinoma. 3-D confocal microscopy was used to investigate the possibility of nuclear localization of HY in MGH cells. The fluorescence of HY was confirmed to be emitted from HY containing cells using spectrometry. The localization of a DNA fluorescent probe 4', 6-diamidino-2-phenylindole dihydrochloride (DAPI) was used to confirm the possibility of colocalization of DAPI and HY. The colocalization analysis in the present study suggests that it was very unlikely that HY colocalized in the nucleus that was stained by DAPI. Fluorescein leakage tests showed that 1% NMP changes the permeability of cell membranes, and enhanced the delivery of HY into cells resulting in lower cell survival ratios. Thus, NMP was able to enhance the photodynamic therapeutic effects of HY on cancer cells.	bladder
[Electromyographic activity increases the bispectral index up to 98 during anesthesia].	We report the patients who developed sudden unpredicted increases of bispectral index (BIS) value during sevoflurane and fentanyl anesthesia. After the epidural catheter placement, anesthesia was induced with propofol and fentanyl, and muscular relaxation was obtained by vecuronium for tracheal intubation. Anesthesia was maintained with 1-1.5% sevoflurane, intermittent administration of fentanyl and epidural infusion of ropivacaine. The nociceptive stimuli might be unchanged during the surgical procedure of reconstruction, but sudden increases of BIS value were found. At first, the BIS was decreased with small dose of supplemental anesthetics, but finally, it was up to 98 and the depth of anesthesia could not be assessed by BIS value. Because slight shivering was found in the patient immediately after emergence, electromyographic activity might have falsely elevated the BIS excessively. No clear recall or explicit memory during operation was observed after anesthesia, but anesthesiologists might better pay much more attentions to unpredictable changes of anesthetic depth during anesthesia.	bladder
Can bladder adenocarcinomas be distinguished from schistosomiasis-associated bladder cancers by using array comparative genomic hybridization analysis?	Bladder cancer is the most common malignancy in many tropical and subtropical areas, correlating well with the endemicity of schistostomiasis. The majority of schistostomiasis-associated (SA) bladder cancers are squamous cell cancers, whereas the majority of non-SA cases in the Western world are transitional cell cancers, suggesting different carcinogenetic mechanisms. Approximately 6% of SA and 1% of non-SA cases are adenocarcinomas. To achieve fine-resolution information of DNA copy number changes in SA adenocarcinomas, 10 tumor samples were analyzed on an oligonucleotide-based CGH array. The frequency of aberrations ranged from 2 to 17, with an average of 10 alterations per sample. The most frequently gained regions were 20q and 8q (in 70 and 60% of the cases, respectively), whereas the most frequently lost regions were 5q and 8p (both in 40% of the cases). In addition, six regions of amplification were found in three samples, containing both well characterized and novel regions. Comparison of the DNA copy number profiles to previously reported profiles of SA transitional cell carcinoma and squamous cell carcinoma revealed similarities (e.g., gains at 5p and 8q), as well as differences (e.g., TCC- and SCC-associated losses at 18p and 20p, and adenocarcinoma-associated gains at 20q). The results suggest that although SA cancers share genetic features, there also exist histology-specific regions of gain and loss.	bladder
Surveillance of patients with bladder carcinoma using fluorescent in-situ hybridization on bladder washings.	OBJECTIVES: To compare the sensitivity and specificity of the UroVysion (Abbott Laboratories Inc., Downers Grove, IL, USA) fluorescent in-situ hybridization (FISH) assay to that of urinary cytology obtained from bladder irrigation during cystoscopic surveillance in patients with bladder carcinoma. PATIENTS AND METHODS: The medical records were retrospectively reviewed for 41 consecutive patients screened at the authors' institution between August 2000 and December 2006 for recurrence of pathologically confirmed bladder cancer. All 162 cytology examinations and 141 FISH assay results obtained from bladder washing were included. Recurrence was determined by cystoscopy, bladder biopsy and upper-tract imaging. Sensitivity, specificity, positive predictive and negative predictive values were assessed using a chi-square distribution with one degree of freedom. RESULTS: There were 24 men and 17 women (male to female ratio 0.59), the mean (range) age was 56 (33-73) years and the mean follow-up 30 (2-57) months. At the initial diagnosis, 35 of the 41 patients (85%) had superficial tumours (stage <or= T1), while six (15%) had muscle-invasive tumours (stage >or=T2). Twenty-six (63%) had low-grade and 15 (37%) had high-grade tumours. In 16 of 141 (11%) of the FISH assays and 16 of 162 (10%) of the cytological samples that were collected from bladder irrigations, there were too few cells for an adequate analysis. The FISH assay correctly correlated with subsequent cystoscopy, bladder biopsy or upper-tract imaging in 110/125 (88%) cases but not in 15/125 (12%). Cytology correctly correlated with the subsequent evaluation in 112/146 (77%) cases but did not in 34/146 (23%). When the FISH was compared with cytology in this setting, the sensitivity was 77% (30/39) vs 74% (37/50; P > 0.1), the specificity was 93% (80/86) vs 78% (75/96; P < 0.01), the positive predictive value was 83% (30/36) vs 64% (37/58; P < 0.05), and the negative predictive value was 90% (80/89) vs 85% (75/88; P > 0.1), respectively. CONCLUSION: The UroVysion FISH assay obtained from bladder washings during cystoscopic surveillance of patients with a history of bladder cancer provides a similar specificity but greater sensitivity than that of cytology for detecting bladder cancer recurrences. Given the better specificity and similar sensitivity of UroVysion compared with urine cytology obtained from bladder washings, a reasonable approach might be to use the UroVysion assay as the primary marker for recurrence, with urine cytology used as a complementary examination.	bladder
Macroscopic, but not microscopic, perivesical fat invasion at radical cystectomy is an adverse predictor of recurrence and survival.	OBJECTIVE: To examine whether the presence of microscopic (pT3a) or macroscopic (pT3b) disease worsens the prognosis relative to pT2 disease at radical cystectomy, as the prognostic significance of pT3a vs pT3b perivesical fat invasion (pT3) is controversial. PATIENTS AND METHODS: In all, 242 patients with pT3 disease (pT3a in 88, pT3b in 121) had radical cystectomy and bilateral pelvic lymphadenectomy for transitional cell carcinoma of the urinary bladder; they were compared with 172 who had organ-confined muscle-invasive disease (pT2). For the analyses we used univariable and multivariable Cox regression models of recurrence and cancer-specific survival, adjusted for age, tumour grade, lymphovascular invasion and the presence of lymph node metastases. RESULTS: In multivariable analyses, microscopic perivesical fat extension (pT3a) was not associated with higher recurrence (P = 0.3) or the mortality rate (P = 0.06) vs pT2 disease. Conversely, the presence of deep perivesical fat extension (pT3b) was associated with 1.8 times the rate of recurrence (P = 0.002) and with twice the rate of death (P = 0.001) vs pT2 disease. CONCLUSION: These findings imply that a detailed assessment of the cystectomy specimen for the presence of microscopic perivesical fat invasion might not be necessary, as the presence of pT3a disease has no strong effect on recurrence or mortality. Moreover, patients with pT3a disease might not require more aggressive therapy than their counterparts with pT2 disease. However, further validation of our data is required.	bladder
[Bladder leiomyoma. Report of two new cases and bibliographic review].	OBJECTIVE: Primary mesothelial tumors of the bladder are rare entities, being leiomyoma the most frequent of them. It may grow without any clinical symptoms or with very unspecific ones. METHODS: We report two cases of bladder leiomyoma in 2 patients, and performed a bibliographic review. RESULTS: They were treated by transurethral resection, with an excellent outcome, without incidences over five years of follow-up. CONCLUSIONS: Surgical treatment continues to be the best solution; it is easy to perform and has a very limited number of complications. Prognosis is good in any case and progression is anecdotic.	bladder
[Inverted papilloma. Report of two cases and bibliographic review].	OBJECTIVE: Inverted papilloma is a rare polypoid lesion of the urothelium, known as a different entity since 1963. Not many more than 100 cases have been published. Although its malignant potential is still unknown, several scientific publications have noted it. METHODS: We report to cases of inverted papilloma and perform a detailed bibliographic review. RESULTS: Both patients were diagnosed by the Pathology Department and entered periodic follow-up visits, being free of recurrence at 24 and 18 months respectively. CONCLUSIONS: The possibility that inverted papilloma is a lesion with malignant potential should be considered in every case, and we suggest that patients with this diagnosis should enter a follow-up protocol like any bladder neoplasia.	bladder
Chalcone arrests cell cycle progression and induces apoptosis through induction of mitochondrial pathway and inhibition of nuclear factor kappa B signalling in human bladder cancer cells.	Chalcones (1,3-diphenyl-2-propenone) are cancer preventive food components found in a human diet rich in fruits and vegetables. In this study, we first report the chemopreventive effect of chalcone in two human bladder cancer cell lines: T24 and HT-1376. The results show that chalcone inhibits the proliferation of T24 and HT-1376 cells by inducing apoptosis and blocking cell cycle progression in the G2/M phase. Western blot assay showed that chalcone significantly increases the expression of p21 and p27 proteins, and decreases the levels of cyclin B1, cyclin A and Cdc2, thereby contributing to cell cycle arrest. In addition, chalcone increased the expression of Bax and Bak, but decreased the levels of Bcl-2 and Bcl-X(L) and subsequently triggered mitochondrial apoptotic pathway (release of cytochrome c and activation of caspase-9 and caspase-3). Our study suggests that the induction of mitochondrial pathway and inhibition of the nuclear factor kappa B survival system may play important roles in the antiproliferative activity of chalcone in T24 and HT-1376 cells.	bladder
Using satellite derived land cover information for a multi-temporal study of self-reported recall of proximity to farmland.	"Exposure misclassification is a major concern in epidemiologic studies. The potential for misclassification becomes even more problematic when participants are asked to recall historical information. Yet, historical information is important in cancer studies, where latency is long and causative exposures may have occurred years or even decades prior to diagnosis. Even though self-reported proximity to farmland is a commonly used exposure measure, the accuracy of recall is seldom, if ever validated. Geographic Information Systems (GISs) and land cover information derived from satellite imagery can allow researchers to assess the accuracy of this exposure measure, and to quantify the extent and importance of exposure misclassification. As part of a bladder cancer case-control study in Michigan, participants were asked whether they lived on a farm, or within a distance of 1/4, 1/4-1, 1-5, or >5 miles from farmland for each residence over their lifespan. Responses from 531 participants over two time periods--1978 and 2001--were investigated. Self reported proximity to farmland was compared to a ""gold standard"" derived from Michigan land cover files for the same time periods. Logistic regression and other statistical measures including sensitivity, specificity, and percentage matching were evaluated. In comparing self-reported and land cover-derived proximity to farmland, cases exhibited better agreement than controls in 2001 (adjusted OR=1.74; 95% CI=1.01, 2.99) and worse agreement in 1978, although not significantly (adjusted OR=0.74; 95% CI=0.47, 1.16). When comparing 2001 with 1978, both cases and controls showed better agreement in 2001, but only cases showed a significant difference (adjusted OR=2.36; 95% CI=1.33, 4.18). These differences in agreement may be influenced by differences in educational attainment between cases and controls, although adjustment for education did not diminish the association. Gender, age, number of years at residence, and geocoding accuracy did not influence agreement between the proximity approaches. This study suggests that proximity measures taken from satellite-derived land cover imagery may be useful for assessing proximity to farmland, and it raises some concerns about the use of self-reported proximity to farmland in exposure assessments."	bladder
Artificial intelligence for predicting recurrence-free probability of non-invasive high-grade urothelial bladder cell carcinoma.	The objective of our study was to define a neural network for predicting recurrence and progression-free probability in patients affected by recurrent pTaG3 urothelial bladder cancer to use in everyday clinical practice. Among all patients who had undergone transurethral resection for bladder tumors, 143 were finally selected and enrolled. Four follow-ups for recurrence, progression or survival were performed at 6, 9, 12 and 108 months. The data were analyzed by using the commercially available software program NeuralWorks Predict. These data were compared with univariate and multivariate analysis results. The use of Artificial Neural Networks (ANN) in recurrent pTaG3 patients showed a sensitivity of 81.67% and specificity of 95.87% in predicting recurrence-free status after transurethral resection of bladder tumor at 12 months follow-up. Statistical and ANN analyses allowed selection of the number of lesions (multiple, HR=3.31, p=0.008) and the previous recurrence rate (>or=2/year, HR=3.14, p=0.003) as the most influential variables affecting the output decision in predicting the natural history of recurrent pTaG3 urothelial bladder cancer. ANN applications also included selection of the previous adjuvant therapy. We demonstrated the feasibility and reliability of ANN applications in everyday clinical practice, reporting a good recurrence predicting performance. The study identified a single subgroup of pTaG3 patients with multiple lesions, >or=2/year recurrence rate and without any response to previous Bacille Calmette-Guérin adjuvant therapy, that seem to be at high risk of recurrence.	bladder
[Evidence of occurrence of gap junctions in stable nonobstructed human bladder].	OBJECTIVE: To investigate the occurrence of gap junctions and the gap junction forming protein connexin (Cx) in normal human detrusor. METHODS: Specimens of detrusor smooth muscle were obtained from 6 patients with localized bladder tumors during radical cystectomy. Transmission electron microscopy and freeze fracture microscopy were used to observe the microstructure of the detrusor smooth muscle. Reverse transcription polymerase chain reaction (RT-PCR) and fluorescence immunohistochemistry was used to detect the expression of Cx in the detrusor smooth muscle. RESULTS: Ultrastructural and freeze fracture studies showed gap junctions in the detrusor smooth muscle cells. However, these gap junctions appeared to be small and irregularly shaped. RT-PCR amplified 2 fragments with the lengths of 300 bp and 200 bp, and sequencing showed that they were identical with the Cx45 already published. Fluorescence immunohistochemistry showed the existence of immunological signal of Cx45 in most of the detrusor smooth muscles. CONCLUSION: The smooth muscle cells of stable human detrusor are electrically coupled through gap junctions.	bladder
Cystectomy in the ninth decade: operative results and long-term survival outcomes.	INTRODUCTION: Radical cystectomy (RC) with urinary diversion remains as one of the more complex urological procedures despite considerable progress in surgical technique. Increasing patient age, along with associated age-related comorbidities, may portend a poor outcome in those undergoing such complicated surgical procedures. Herein, we report our experience with radical cystectomy in the elderly population. METHODS: We retrospectively reviewed our RC results from 1995 to 2003. Patients >or = 80 years old were included in this analysis. Perioperative outcomes, as well as overall and disease-free survival were evaluated. RESULTS: A total of 517 patients underwent RC with urinary diversion during this time period. Forty-nine (9.5%) patients were >or= 80 years old. Mean age and BMI were 83.4 years (range 80-94) and 27.1kg/m2 (range 17.4-39.0), respectively. Eighty-three percent of the patients had >or= 1 comorbidities and 67% had a significant smoking history. Mean operative time and estimated blood loss were 279 minutes and 985 ml, respectively. Thirty-two patients (76%) required blood transfusion in the perioperative period. Among patients found to have urothelial cancer a pathological analysis (36), 21 patients (58%) had < pT3a while 15 patients (42%) had >or= pT3b or >or= N1. Intraoperative complications (5%) included one large bowel injury and hypogastric artery laceration. Thirty- and 90-day mortality rates were 9.5% and 11%, respectively. Early and late postoperative complications were 57% and 17% and 5-year overall and disease-free survival were 44% and 36%, respectively. CONCLUSIONS: Radical cystectomy with urinary diversion in patients >or= 80 years old is related with significant short-term and long-term morbidity. Proper patient selection assessing performance status and psychosocial parameters appear to optimize survival outcomes. However, regardless of age, timely surgical management for localized disease control is essential for ultimate sustained disease-free survival.	bladder