News Finding: And thanks to their newly-developed in vitro approach, in future it will be possible to identify other proteins that operate in the fusion process.
Paper Finding: 3 G) , suggesting that elevated autophagy is not required to induce the fusion signal.
News Context: These results help to understand autophagy and its underlying molecular processes better. And thanks to their newly-developed in vitro approach, in future it will be possible to identify other proteins that operate in the fusion process. Note: material may have been edited for length and content.
Paper Context: Finally, the fusion frequency was similar with cytosolic fractions isolated from cells grown under nutrient-rich conditions and from rapamycin-treated cells ( Fig. 3 G) , suggesting that elevated autophagy is not required to induce the fusion signal. Taken together, we show that autophagosome-vacuole fusion can be recapitulated in vitro, with ypt7Δ autophagosomes, atg15Δ vacuoles, energy, and at least one soluble cytosolic factor.