News Finding: Interestingly, our results revealed that approximately 15 % of the cell populations were TUJ-1 positive, whereas GFAP expression was detected in only a few cells.
Paper Finding: Surprisingly, in our cell culture conditions, in the absence of any kind of differentiation stimuli, some cells derived from EC-MSCs displayed a typical neuronal and glial-like morphology.
News Context: Surprisingly, in absence of any stimuli, some adherent cells closely resembling neuronal and glial morphology were also observed. Interestingly, our results revealed that approximately 15 % of the cell populations were TUJ-1 positive, whereas GFAP expression was detected in only a few cells. Furthermore, TEM analysis confirmed the stemness of EC-MSCs and identified some cells with a typical neuronal morphology.
Paper Context: These findings confirm that a high percentage of EC-MSCs are naive and undifferentiated sharing ancestral or primitive characteristics . Surprisingly, in our cell culture conditions, in the absence of any kind of differentiation stimuli, some cells derived from EC-MSCs displayed a typical neuronal and glial-like morphology. Interestingly, immunofluorescence analysis confirmed our observations under phase contrast microscopy, showing that approximately 15 % of the cell populations were TUJ-1-positive with a fibrillar cytoplasmic staining among the cells displaying neuronal-like morphology, whereas the GFAP expression was detected in both the cytoplasm and the cytoplasmic extensions of only a few cells.