News Finding: Flow cytometry analysis demonstrated that EC-MSCs expressed CD90, CD105, and CD73, while being negative for the leukocyte common antigen CD45.
Paper Finding: EC-MSCs showed high expression for CD90 (96.3 %), moderate expression for CD105 (64.31 %), and low expression for CD73 (23.09 %) while were negative for the leukocyte common antigen CD45.
News Context: Differentiation assays proved that EC-MSCs are able to differentiate towards mesodermal lineages (osteogenic, adipogenic, chondrogenic). Flow cytometry analysis demonstrated that EC-MSCs expressed CD90, CD105, and CD73, while being negative for the leukocyte common antigen CD45. Immunofluorescence analysis showed a high percentage of positive cells for OCT-4 and SSEA-1.
Paper Context: Actually, while the differentiated cells die within two days after death, the MSCs reside in their hypoxic tissue niche in a state of quiescence or dormancy, and survive by adapting to a low rate of oxygen consumption with a slow Fig. EC-MSCs showed high expression for CD90 (96.3 %), moderate expression for CD105 (64.31 %), and low expression for CD73 (23.09 %) while were negative for the leukocyte common antigen CD45. Consistently, anoxia and a lack of nutrients participate in the positive selection of more robust and undifferentiated stem cells .