News Finding: "In less than 24 hours, because we'd already sequenced Chief and his carrier son, we were able to identify a 'nonsense' mutation on chromosome 5.
Paper Finding: If available, sequence for an animal homozygous for Chief's normal haplotype could also rule out those mutations.
News Context: He and coauthor Heather Adams were able to quickly identify a candidate. "In less than 24 hours, because we'd already sequenced Chief and his carrier son, we were able to identify a 'nonsense' mutation on chromosome 5. It was a eureka moment," Lewin says.
Paper Context: Chief, Mark, Ivanhoe Chief, and Valiant were sequenced to ~31×, 13.5×, ~50×, and ~36× coverage, respectively (Supplementary Table  S1 ; http://dx.doi.org/10.3168/jds.2015-10517). If available, sequence for an animal homozygous for Chief's normal haplotype could also rule out those mutations. Analysis of Chief's DNA within the critical region revealed 3 unique SNP in exons, with the rest being intronic, intergenic, 3 UTR, and downstream regions (Table 4 ).