In the table below, indicate which group of pigs you believe will now be susceptible to inoculation by the indicated viruses. You only need to name the technique. You do NOT need to describe how it works. The experiments reported in June of 1997 in J. Virology by Dove and Racaniello challenge the widely held model for picornavirus entry and uncoating. The following three questions are based upon your knowledge of that paper.
Sedimentation is from right to left.
Dove and Racaniello found that, in their case, some ca mutants had lesions in the viral protein 2C, a non-structural protein. Earlier evidence has implicated this protein in both genome replication and virion assembly. Assuming that the alteration in the case of the ca mutants is at the level of assembly, outline an experiment that might determine of ca mutant virions are , in fact, assembled differently than PV1 virions. Use X-ray crystallographic technizes to reslove structure of these particles. In other experiments, Dove and Racaniello reported that some ca mutations may be found in the 3D protein, the viral RNA polymerase.