The small, medium and large RNA encode the viral N, G1,G2 and L proteins, respectively. Each RNA segment is complexed with the viral N protein forming a nucleoprotein template employed in both transcription and replication. RNA synthesis is catalyzed by the viral L protein. Two glycoproteins, G1 and G2 are embedded in the virion membrane. Our research is focused on elucidating structural organization of the L protein and defining the mechanisms of L protein catalyzed transcriptase and replicase activity.
Schistosomes undergo a complex developmental pathway.
Our goal is to understand the mechanisms employed by mediators of development and cellular differentiation in Schistosoma mansoni . RXR-2, a member of the retinoid X receptor family in Schistosoma mansoni . Identification of a cDNA encoding a RXR homologue from Schistosoma mansoni . Evidence for a role in female-specific gene expression. Use of lysolecithin permeabilized infected cell extracts to investigate the in vitro biochemical phenotypes of poxvirus ts mutations altered in transcription activity.