A Becton Dickinson FACS 440 flow cytometer provides our users with several capabilities including simultaneous, three-color analysis of cell surface markers and additional potential for DNA analysis. The flow cytometer provides modern typing capability for lymphocytes and tumor cells. An ACAS system consisting of an inverted fluorescence microscope with a laser excitation source is available for analysis and clonal isolation of attached cells. The ACAS is also designed for use as a confocal microscope with digital imaging and 3-dimensional image reconstruction capabilities. A core facility for biotechnological research is available to provide research tools in molecular biology.
Technical training is provided to students and faculty for the effective use of the facility.
DNA oligonucleotide synthesis is accomplished with a Beckman Oligo 1000M oligonucleotide synthesizer, and is usually available within one to two days of ordering. It is a complete system composed of an electrophoresis and detection unit with hardware and software for data display. This protein sequencer combines in one single system all the sequencing capabilities such as pulsed liquid or gas-phase chemistry, microgradient PTH amino acid analysis, and computer data handling. The instrument is specially designed for Fmoc chemistry and yields adequate amounts of material for epitope mapping, antisera pro duction, and biorecognition studies. The Mass Spectrometry Laboratory is avail able for use by students and faculty.