Dr. Steven Birken Black Bldg. The Protein Chemistry Core Facility provides protein sequencing, amino acid analysis, peptide synthesis, and DNA synthesis. Investigators are encouraged to meet with appropriate staff members to discuss sample preparation or peptide design before submitting a request for service. In-gel digestion of proteins separated by 1D or 2D gels followed by mass spectroscopic analysis of the resulting peptide mixture and database search to identify known proteins. Amounts as low as 100ng may be used.
Structure is confirmed by amino acid analysis and, if necessary, by mass spectrometry.
The facility has two Applied Biosystems 394 DNA synthesizers and one 3948 synthesizer. Synthesis is performed at the 40 nmole scale unless a larger scale is requested. Cartridge purification is available upon request. Maldi-Tof mass spectrometric analysis is available to all scientists. The facility has a PerSeptive Biosystems delayed extraction-reflector instrument and samples of 1,000 to 100,000 AMU can be analyzed.