Sublateral bundles are visible as chains of discrete bright spots.
Commissures and the amphid dendritic bundles are not normally visible. Some neuronal cell bodies fluoresce faintly. Several lines of evidence imply that this fluorescence is primarily due to VAMP-GFP localizing to synaptic vesicles. First, VAMP antibody staining in wildtype worms shows that VAMP-GFP fluorescence parallels normal VAMP localization. We have performed a pilot screen for synaptogenesis mutants using jsIs1 and have isolated mutants with axonal elongation or migration problems, with accumulation of label in cell bodies, and with inappropriate patches of fluorescence.