If one is preparing large batches of slides, replace the dH20 periodically. Before removing the slides out of the dH2O, you will want to dip them up and down a few times to make sure they have been rinsed well. Using the forceps, lean the slides against the test tube racks in order for them to dry. Repeat this process until you have coated sufficient slides. The slides should be clear.
White spots are a sign of improper rinsing.
When you are finished dipping the slides, clean up the hood. Do not save the stock solution longer than a couple of hours. Place the stock solution from the stock bottle and first Coplin jar into the appropriate hazardous waste bottle. Rinse these two containers with the acetone from the second Coplin jar. Discard the acetone into the appropriate hazardous waste bottle.