In the Taqman system, flanking primers are used to amplify the polymorphic region, and two competing fluorogenic oligonucleotide probes complementary to each allele are cleaved if they anneal to the amplified fragment. The cleave of the probe matching the allele present in the amplified fragment is enhanced over that of the competing primer. By monitoring the appropriate florescent wavelengths for each fluorogenic probe, the genotype of the homozygous or heterozygous loci can be determined. This endpoint PCR technique permits the rapid screening for a particular SNP loci of interest. The capacity of this system can exceed thousands of genotypes per day.
Some projects require a variety of technical approaches to identify the traits or genes of interest.
Lark will work with the client to incorporate the technology needed into a unified project. Finally, when searching for novel genes, identifying SNP's in a population, or detecting de novo mutations in known gene, Lark's sequencing capacity can play an integral role in identifying key information leading to more complete knowledge of the trait under investigation. All site contents, copyright Lark Technologies, Inc., 1998.