Thus, a specific amount of material at specified conditions will absorb a specific fraction of the light striking it. Determining the response of some analytical method to known amounts of pure analyte. The amount of solute in a given volume of solution, e.g., moles per liter. Transparent receptacle in which sample solutions are introduced into the light path of spectrometers. For UV work, the material is quartz.
Visible work permits the use of glass or plastic cuvettes.
Device used to detect the intensity of the radiation from either sample or reference beams. Usually a simple silicon diode or a more sensitive photomultiplier tube. The number of times per unit time that the magnitude of an electromagnetic wave goes from maximum to minimum then back to maximum amplitude. The distance between grooves and the angle of the faces are determined by the wavelengths to be separated. Lowest amount of analyte that may be seen above noise level of instrument.