The LC should be 80 uA. Turn gun bias dial counterclockwise to increase the LC and clockwise to decrease it. Change magnification to 500x. Press Pic1 on the key pad and adjust contrast and brightness so an image is visible. Press TV mode, decrease magnification as necessary, and move specimen until portion you are interested in is in your field of view. Use the x, y, and z controls on the specimen chamber.
X moves the specimen horizontally on the screen.
Y moves the specimen vertically on the screen. Z moves the specimen perpendicular to the plane of the screen. The r control will also be helpful if you need to rotate your specimen. Tilt control will allow you to inspect the specimen at different angles. At high magnification, the joy stick on the console will give you more fine tuned control in moving your specimen.