Some other dyes can substitute for methylene blue. Make a fresh glucose solution on the day of the experiment. This experiment can be performed by students. The students may shake the solutions in stoppered test tubes. Emphasize the safety precautions.
Skin irritation is possible if exposure is prolonged.
Methylene blue will stain clothing. Go to Top Precautions Wear eye protection. Wash off spills. Wear old clothing or lab aprons. Check that the safety shower and eye wash fountain are in working condition.