It is absolutely imperative that we get the best possible fixation for synapses.
Synapses have a large lipid component.
We have at our disposal UV light and Progressive Lowering of Temperature techniques, as well as standard oven polymerization. We would so much appreciate any help we can get. Our antigens tend to be very difficult to locate with Au in material fixed with osmium and embedded in epoxides, which, of course, would give us our best fixation of synapses. Hildy, You did not indicate whether it is possible to perfuse fix your material. A combined light and electron microscopic immunocytochemical method for the simultaneous localization of multiple tissue antigens.