Analysis Address for samples sent by express mail services and the intercampus courier The PSCF at the University of Nebraska Medical Center is a non-profit service facility providing amino acid analysis, protein sequencing and mass spectrometry on a contract basis. The facility is supported by funds from the Nebraska Research Initiative. Use of the facility is not limited to the University system. Other amino acids can be quantitated upon request.
Samples can be either liquid or blotted onto PVDF membrane.
We perform vapor hydrolysis using 6N HCl with phenol and sodium sulfite added. 20-24 hours of hydrolysis under argon is generally used. Amino acids are then analyzed with a Beckman 6300. The Beckman 6300 uses an ion exchange column to first separate the amino acids. This method may take samples which are contaminated with salts, urea, and small amounts of detergents.