Differential repression of transcription factor binding to nucleosomes by histone H1 is regulated by the core histone amino termini. Binding of disparate transcriptional activators to nucleosomal DNA is inherently cooperative. Acetylation of histone H4 plays a primary role in enhancing transcription factor binding to nucleosomal DNA in vitro. Binding of the wheat bzip protein, EMBP-1, to nucleosomal DNA is modulated by nucleosome positioning. Histone H1 repression of transcription factor binding to a stably positioned nucleosome.
Transcriptional activators target histone acetyltransferase complexes to nucleosomes.
Alteration of nucleosome structure as a mechanism of transcriptional regulation. Purified histone acetyltransferase complexes stimulate HIV-1 transcription from preassembled nucleosomal arrays. The ATM-related cofactor Tra1 is a component of the purified SAGA complex. Regulation of transcription by multisubunit complexes that alter nucleosome structure. Activation domain specific and general transcription stimulation by native histone acetyltransferase complexes.