Nutlets disperse by adhering to tires, shoes and clothing of people, fur, feathers, and feet of animals. Germination requires warm temperatures. The largest seed in a nutlet is usually the first to germinate. Other seeds may germinate or remain dormant depending on moisture availability. Buried seed can remain viable for several years.
Seedlings emerge early spring through summer, often in flushes following increased soil moisture.
Seedlings develop a deep root system in a few weeks, and flowers may be produced within 3 weeks, burrs within 6 weeks. In tropical regions, plants develop woody roots and become perennial. Removal of plants with burrs and repeated cultivation to prevent burr formation or planting competitive vegetation can help control infestations. Puncturevine is unlikely to be confused with other weeds.