Polarized emission is increased when the molecular rotation of a fluorophore is reduced. The magnitude of the polarized signal relates quantitatively to the extent of fluorescent ligand binding. FP assay yields equivalent MC3 agonist rank order potencies as published MC3 data using radioligand as tracer. 2 Test Kits cover a range of characterized receptor-ligand combinations developed and validated in fluorescence polarization based binding assays. Ideal for researchers who want to test this platform in their own lab and to gain experience implementing and optimizing a FP based binding assay.
Special pricing is possible based on the number of compounds screened.
A selected receptor-ligand combination can be rapidly evaluated for its potential as a primary screening target, and further developed into a comprehensively validated package which is transferred to the customer for evaluation before commitment to screening. Recommendations for optimizing the principle components of the assay.