Do not leave tubes open on bench. Place eppendorf tube containing oligonucleotide in heated vacuum centrifuge. Leave on dry ice for 15-20 mins. Decant supernatant from pellet. Measure O.D. at 260nm to determine concentration.
Specific primers exist.
The sequence can be efficiently amplified by PCR ie. A duplicate reaction is set up, as a positive control, without the 32P dCTP and replacing the hot dNTP mix with the 10x dNTP mix. The positive control is run out on a mini gel, to check that an amplified product was produced and also that a single band was produced with no contamination. The length of the extension time, depends on the length of sequence to be amplified. The theoretical maximum efficiency of DNA Taq polymerase is 3.6Kb per minute.