Lay the flat plate on the right end of the apparatus, scratched side facing down. Place two white spacers along the edges of the back plate and secure with small black clips. Place the notched plate at the far left end of the apparatus and let it overlap on the back plate, scratch mark facing up. Acrylamide 100.0 ml 10X TBE Add urea, water, and acrylamide and stir into solution over a warm water bath. Con tinue to stir for 10 minutes.
Vacuum filter the slurry and collect solu tion.
Add 10X TBE to solution. Make up difference to 1 liter with dH20. Mix the solutions together and then draw it up in a 60-cc syringe. 1.99 ml dH2O Add 1.00 g of ammonium persulfate with dH2O to 10 ml. 3 -Slowly dispense the acrylamide mix onto the back plate and push the notched plate over it.