Also covers the separation of chiral compounds, and assay requirements and techniques for the sensitive and accurate measurement of drugs and metabolites in biological matrices, with emphasis on pharmacokinetics and biopharmaceutical applications. The class discusses and uses computer programs to analyze data representing actual experimental situations. The methodologies covered include ultraviolet-visible, infrared, nuclear magnetic resonance, and mass and fluorescence spectrometry. The class includes discussions of physical principles, instrumentation involved, exercises in the interpretation of spectrometric data, and examples of applications. The course includes laboratory experiments to exemplify the techniques discussed in the lectures.
Students write and submit reports in a selected pharmacology journal format.
Topics include tissue culture, radioisotopes, signal transduction, radioligand binding, drug metabolism, protein and nucleic acid identification and quantification, electrophysiological, and in vivo techniques. Pharmacodynamics is the study of the biochemical and physiological effect of drugs on biological systems. The course covers mechanisms by which pharmacological agents interact with the living organism to provide the student with a rational basis for investigations in biomedical research. Topics include the pharmacodynamics of drugs influencing the central and peripheral nervous system, and the endocrine, renal, respiratory, and cardiovascular systems. Lectures supplement weekly conferences and discussion groups.