This 5 mL sample was homogenized with 50 mL of deionized water and then enumerated using the above technique. Slides are enumerated using the ocular reticule described above. The amount of time each slide is in the canal is given. Results are an average of 4 separate counts. Slides are enmerated using the ocular reticule and magnification described above.
Time spent in the canal is given.
Cells or colonies enumerated by filtering 10 mL of sample through a 0.22 micron millipore filter and all of the filtrate counted and identified. Those species too numerous to count using the above method. In these cases, a Neubauer Improved hemacytometer was used. Slides are enumerated by taking all of the attached growth on a slide and homogenizing with 10 mL of deionized water. This is then enumerated using a Neubauer Improved hemacytometer.