Teacher can remove gels after they have run sufficiently and place them in baggies or saran wrap until ready to do the staining. 55 minute period to stain and destain the gels, and to analyze. Teacher can shorten this time period by doing the staining and destaining if not able to devote to full periods to this activity. If students have not used a micropipettor and have not loaded samples into a gel, then time must be spent practicing these techniques prior to doing this lab . To practice loading wells the teacher can pour some agarose into petri dishes that have combs in place.
Students can use a mixture of loading dye and water to practice loading gels.
BamHI 20 ul. HindIII 120 ul. 2x compromise restriction buffer 20 ul. 5 liters 1X TBE Buffer Carolina Blue stain 50 microcentrifuge tubes 10 0.5 - 10 ul. The 200 ul.