This method is advantageous for saving the occasional cultures that become contaminated. Yeast contaminated cultures will appear cloudy when slightly shaken and lymphocytes will not cluster together as much as normal. If cultures are suspect, a drop of culture can be streaked on a YPD media plate to check for growth of yeast colonies, or a 5 ml sample can be taken to Barnes Diagnostic Center for identification of yeast strain. To keep a written and computerized record of all cell lines, the dates when cell lines were received and frozen, freezer locations, and any other important information such as dates of birth, sex, etc. Sample problems for determining cell number, cell density, viability, etc.
Some cells stick to coveslips better if they are coated with poly amino acids or collagen.
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