Click here for more information for methods of sample preparation. Amounts as low as 100ng may be used. Click here for more information for methods of sample preparation. Includes MS analysis of the dig est and selected peptides. Samples submitted fo r the Beckman analyzer may contain salt, but those for the ABI 420 must be salt-free.
Structure is confirmed by amino acid analysis and, if necessary, mass spectrometry.
The facility has three Applied Biosystems 394 DNA synthesizers. Synthesis is performed at the 50 nmole scale unless a larger scale is requested. Cartridge purification is available upon request. Maldi-Tof mass spectrometric analysis is available to all scientists. The facility has acquired a PerSeptive Biosystems delayed extraction-reflector instrument.