Flaps of epithelial tissue still attached. About 5 mL of blood with anticoagulant. If the specimens can be delivered to a laboratory within 24 hours, they should be placed on ice. If delivery will take longer, quick-freeze the specimens and do not allow them to thaw during transit. If dry ice is used, be sure that the vials are tightly sealed with stopper and tape so that no carbon dioxide enters the vial.
Serology is complicated by cross-reactions with other undefined porcine enteroviruses.