The purified enzyme is free of these traces and is recommended for analytical use in the presence of di- or polysaccharides. Considerable work has been done on immobilized glucose oxidase. Tsuge et al . The a anomer is not acted upon. Dry preparations are stable for years when stored cold.
Solutions are reasonably stable under a variety of conditions.
The reaction velocity is determined by an increase in absorbance at 460 nm resulting from the oxidation of o-dianisidine through a peroxidase coupled system. One unit causes the oxidation of one micromole of o-dianisidine per minute at 25deg. C and pH 6.0 under the conditions specified. Handle with care. Saturate with oxygen for 10 minutes within 30 minutes of use.