Data will be collected twice a month from each sampling site throughout the school year. Time and days will vary according to scheduling restraints. The following water quality variables have been selected for there importance in the assessment of the types of potential non-point source pollution in the watershed. Physical and Chemical variables 1. Dissolved oxygen will be measured to assess biological health.
Temperature is a biological indicator.
The bottle will then be turned upright into the current and filled completely. Samples are preserved in an ice chest for maximum holding time of 1hr. D. Fecal coliform membrane filter procedure using a Millipore Sterifil Aseptic System and Portable Single Chamber Incubator. Using the sanitized forceps, place an absorbent pad in the presterilized petri dish. Unscrew the neck of the broth plastic tube with the ampoule breaker and drain the broth onto the pad.