Rinse slides in distilled water and air dry with compressed air or use a blow dryer. Check the slides using a Zeiss Micoscope, 100X plan-apochromatic oil objective, brightfield. Adequately trypsinized chromosomes will show telomeres not overly digested and G-Bands will appear sharp and in contrast. If slides are under-treated with trypsin, destain the slides before rebanding. Rinse in distilled water, air dry and reband.
Transfer slides to microslide box or suitable storage container.
Aliquot into 1 ml quantities and store at -20 degrees C for up to 1 year. Thaw just before using. Lowering the temperature of the trypsin in the working salt solution will lower its activity, thus requiring longer exposure times and also increasing the life of the solution. Test slides should be run to determine optimum exposure times. 1 tablet is dissolved in 1 liter distilled water.