We inserted GFP cDNA into two separate strains of mouse melanoma. The amelanotic strain was disappointing and showed little or no fluorescence. Our strain produced approximately six viable subclones of antibiotic resistant cultures. Two of these show particular promise in their metastatic abilities in whole animal experiments. All six are frozen and awaiting further analysis.
Work continues to enhance the fluorescence as well as re-insertion of newer brighter fluorochromes.
We also attempted to insert fluorescent beads into the cells, but the process damaged the cells. We could get the beads into the cells, but they no longer were metastatic. This process may still be useful since the beads are bright enough to be detected in whole animal or tissue slices. She is currently starting her first year in medical school. Eric Armstrong completed the work on the fluorescent beads and also worked with the GFP insertion.