If the protein is bound to the DNA, it will cover the nucleotides at its binding site and protect the fragment from cleavage. The labeled fragments that terminate at the binding site will be missing. Assay Protein molecules bound to the DNA will cause it to migrate slower through the gel, thus indicating the presence of DNA binding proteins. This technique detects the hybridization of a specific mRNA strand to a radiolabeled DNA probe. Cancer cells often are said to be immortal because they lack the normal cell death and senescence pathways.
Telomeres are the protective caps on the ends of chromosomes.
As cells divide, the telomeres shorten in length because DNA polymerase is unable to complete the synthesis of the end of the strand. Telomerase is an enzyme that counter-acts this by providing a means of finishing the strand. Without telomerase, the DNA strand will become shorter everytime it divides and it will eventually die. Addition of the 5 cap. Addition of the Poly A tail.