Sorbitol is a major photosynthetic product in several members of the Rosaceae family. Once S6P is produced by NADP-S6PDH, the phosphate group of S6P is removed by a phosphatase to yield sorbitol. Additionally, a genomic library of Malus X domestica cv. DNA of positive clones was digested with different restriction enzymes and confirmed via Southern analysis.
Work is in progress to characterize non-coding and upstream and downstream regulatory sequences.
Table 1 Characteristics of genomic nucleotide sequence of NADH-S6PDH from apple. Genomic DNA of 35-41 kbp size was cloned in the BamH I site of SuperCos 1. The total length of the structural region is 3400 bp and is intervened by 5 introns, resulting in 6 translated exons. Exon 1 starts with ATG, the translation start codon, and exon 6 ends at TAA, the translation termination site. Accumulation and translocation of sobitol in apple phloem.