Students determine the molecular weight of two unknown acids, one monoprotic and the other diprotic. Students identify unknown ions based on the Rf values. By plotting a graph of ratio to temperature, the optimum ratio is obtained. Given samples of sugars, starches and proteins, students determine the purpose of an iodine test, a Lugol's reagent test and a Biuret test. The students then identify which of these biochemicals are present in an unknown.
Students then determine the concentrations of three unknowns from the curve.