The samples would consist of small pieces of muscle tissue, along with data on time, place and fish size. Tissues would be preserved in alcohol. Both MtDNA and microsatellite techniques would be applied. Peter M. Grewe and John Hampton. Introduction Knowledge regarding population subdivision is central to sustainable fishery management.
Uncertainty regarding bigeye stock structure seriously restricts the confidence that scientists and.
At a national or sub-regional level, fisheries managers need to have a better idea of the broader surrounding population of bigeye from which the fish in their fisheries are drawn. Examination of mitochondrial DNA markers is now an established technique for elucidating population genetic structure. Until recently, variation in the much larger nuclear genome has been chiefly assessed through allozyme electrophoresis, but more powerful methods are now available. Pre-eminent among these is the detection and analysis of microsatellite variation. Microsatellites show high levels of genetic variation and high mutation rates, meaning that populations are likely to diverge not only by genetic drift but by mutation as well.