For those of you who are interested, here is a terrific link with more information. B. Restriction endonucleases are enzymes found naturally in many bacteria to protect the bacteria agains virus infection. C. Two steps occur in order to bind the two pieces of DNA together 1. The recombinant molecule must then be transferred into a living cell where it can be expressed. There are several ways of getting DNA into other cells 1.
Some cells can take up naked DNA by transformation, as bacteria do.
Protoplast fusion, in which plant or bacterial cells that have been made wall-less by enzymes fuse. See Fig 9.5. If the base sequence is known, DNA can be artificially synthesized by a machine using the various nucleotides and other reagents and enzymes. Selecting a clone from a library A. Blue-white screening Fig 9.11 1. The plasmid also contains the beta -galactosidase gene and a restriction enzyme is used which will make cuts within this gene.