Studies range from in vitro molecular experiments to in vivo human clinical trials.
In vitro experiments involve structure-function studies of cloned human kinin receptors and studies of how receptors transmit signals intracellularly. In particular, we are interested in how bradykinin B1 and B2 receptors interact with specific G-proteins. We are also studying the actions of kinins in vivo. We are using airway challenge techniques to understand the pharmacological mechanisms by which they exert their effects. These studies focus on the role of sensory nerves in the actions of kinins and on how sensory nerve responsiveness to kinins is modified by inflammation.