Most separations occur in twenty minutes or less. Fast separations are a result of the centrifugal action of the spinning Rotor driving the mobile phase through the adsorbent layer. The velocity of the mobile phase enables the use of smaller particle adsorbents. This smaller particle bed versus the 35-75 micron cut typical of low pressure column chromatography allows a higher degree of separation efficiency. Centrifugal action combined with the use of a solvent pump to apply the mobile phase allow complete control of solvent velocity profile.
Tight bands mean eluted fractions can be collected in a minimum volume of solvent.
The solvent metering pump also makes the use of step gradients easy to perform with the CycloGraph. The inlet of the pump may be switched from a weak solvent to a more polar solvent during a run. This enables compounds with a wide range of polarities to be separated. Complex samples such as natural products are quickly separated into component classes. Sweeping the polarity of the mobile phase from weak to strong makes it easy to reuse a Rotor for many sample separations.