Decant into the sieves. Back-rinse the sieves into fingerbowls and examine with a dissecting microscope. The tardigrades are found in the 120 and 90 micron mesh sieves, depending on the size of the tardigrade. Eggs will mainly show up in the last sieve. Using a polyethylene wash bottle, I backwash each sieve into a separate fingerbowl.
Some workers place tardigrades directly into the mounting medium.
I find that this causes some shrinkage of the specimens and prefer to dehydrate the specimens using the technique of Seinhorst, 1959. After all have been placed in the dish, remove as much water as possible. Mounting on Microscope slides Specimens can then be mounted in one of the modifications of Hoyer's mounting medium. Since this is an aqueous medium, the edges of the cover glass must be sealed to prevent drying. Let the slides set for several days so that the medium around the edge of the coverglass slightly solidifies.