Tumor cells in a low pH environment are the most difficult to eradicate during therapy.
This would enable us to learn how to manipulate ion transport in these cells to selectively lower intracellular pH, which compromises cell viability, to make cancer therapy more effective. We also examine the effects microenvironmental pH and bicarbonate concentration on gene expression using differential display of mRNA transcripts to identify new therapeutic targets. Ongoing work includes melanoma, breast cancer, and brain tumor cell lines. Effects of 42EC Hyperthermia on intracellular pH in ovarian carcinoma cells during acute acidification or chronic exposure to low extracellular pH. Int. Temporal association between alterations in proton extrusion and low pH adaptation.