This oxygen enrichment has boosted our M13 growth by a factor of five over standard methods. Growth time is approximately 16 hrs and can be set internally in the shaker electronics. All M13 growth at our center is now performed with this shaker. It has been in operation since May 1997.
Typical growth is approx.
If the starting number of cells is too low, the oxygen atmosphere may inhibit cell growth. If you expect low starting cell concentrations, allow growth to proceed for a few hours before turning on the oxygen. Typical growth is approx. If growth is insufficient, try turning oxygen off entirely. You may then slowly add oxygen on subsequent runs to determine the optimum level for your cells and media.