In cell lines lacking G2, cAMP-induced translocation is blocked, indicating that G2 mediates PKC activation. In cells that overexpress RAS in the active GTP-bound conformation, PKC is constitutively active. Biochemical and immunological studies indicate the existence of a second PKC isoform of 65 kD in the nucleus, which is stimulated by phosphatidylserine. Disruption and overexpression of this gene are in progress. This activation is absent in G4 null mutants, and is increased in cells that overexpress RAS in GTP-bound form.
Universal signals control slime mold stalk formation.
USA 91, 8219-8223. Differentiation 58, 95-100. Development 120, 1997-2002. Two cAMP receptors activate common signaling pathways in Dictyostelium. Cell 5, 703-711.