Back to top Sample resuspension 1. This can be done up to 8 hours prior to loading, where the AM shift adds water to samples for PM shift and vice versa. Cover the trays with cap mat sealers and store at 4 C until just prior to loading. Cover trays with cap mats. Set floor vortexer to medium speed and vortex for 5 minutes.
Samples are ready to load.
In cases where samples must be resuspended immediately prior to loading, please be sure to add a vortexing step and quick spin following the initial addition of water. Back to top Fluid level checks before beginning a new run 1. Before filling buffer container rinse with hot water then rinse with Di water. The metal weight at the end of the line should be resting on the bottom of the container. If the buffer level drops below the end of the feed line, then the run will likely result in abnormal and useless data .