A strain of E. coli is ampicillin resistant. You want to know if it can transfer resistance by conjugation to Salmonella. Salmonella is able to utilize citrate as a source of carbon and energy. E. coli cannot do this. Describe how you would determine if your additive was a potential carcinogen.
Some viruses are able to cause cancer.
Describe two ways in which retroviruses can cause a cell to become cancerous. A gene for kanamycin resistance is found on the chromosome of a pathogenic bacteria. You know that it is found on a single EcoR1 fragment. You wish to clone that gene into the EcoR1 site of a plasmid vector, pUC8. When the EcoR1 site is inactivated, the transformant colonies are white on indicator plates, rather than blue.