Factors affecting development of CTLs include dose and frequency of vector administration, MHC type of the host and the presence of memory CTLs.
Studies suggest that injured epithelia more readily take up Ad vectors.
Polycations increase the effciency of adenovirus-mediated gene transfer to epithelial and endothelial cells in vitro. Towards gene therapy for cystic fibrosis, In Cystic Fibrosis-Current Topics. The Challenge of Fetal Gene Therapy. Nat Med 1, 864-866. Complexes of adenovirus with polycationic polymers and cationic lipids increase the efficiency of gene transfer in vitro and in vivo.