The IR laser is turned off after triggering, to reduce background during the subsequent measurements. After 50 m s of PDP background data has been acquired, the PDP modulator opens to start the fluorescence induction measurement. The classification pulse stretchers are reset after the opening of the shutter, but before the cell passes through the strong beam. The IR pulse stretcher is reset after the IR laser has been turned back on for the next sample. PDP microfluorometric measurement of a pair of Phaeocystis cells collected in the Ross Sea, south of New Zealand.
Several cell pairs from a colony viewed under the measurement conditions.
Time course of relative cell fluorescence yield. PDP microfluorometric measurement of a Corethron cell. The same field at 10-fold lower magnification reveals the entire Corethron cell as well as part of a Phaeocystis colony. Time course of cell fluorescence yield, averaged over 50 flashes. Time course of cell fluorescence yield for the first flash in the measurement series.