Use in wholemounts for anterior neural crest, Schuh et al., 1993. Photos by Tim Schuh. A stage 23 embryo, lateral view.
Stain is only obvious in the head crest.
Once again, only head crest is obvious. Compare to xAP2 which stains more posterior crest in addition to the head. The four head crest streams are obvious. The most anterior is the mandibular crest segment moving around the developing eye. The second-most anterior segment is the hyoid crest.