The isolated preparations were lyophilized. The extent of the modification is sufficient to conclude that at least one modified CAT subunit was included into the catalytically active enzyme tetramer. The data suggest that the efficiency of the derivatives be tested in vivo . Elution profiles of CAT and SOD preparations on a Sephacryl S-300 column. SDS electrophoretic separation of CAT and SOD preparations.
Vertical lines correspond to the positions of the marker proteins during electrophoresis.
Due to stretching, the profile was separated into three volumes after the fractions indicated in Fig. 1 d were pooled. However, electrophoretic separation of the derivatives was not performed. The data do not allow more firm conclusions on the organization of the conjugate composition. Comparative study of the efficiency of various preparations of CAT and SOD in vivo should clarify this point.