High insulin and glucose levels, which are present in patients with type II diabetes, have an additive effect in infragenicular VSMC proliferation in-vitro.
Thiamine is a co-enzyme important in intracellular glucose metabolism.
The objective of this study is to determine the effect of Thiamine on human infragenicular VSMC proliferation induced by high glucose and insulin levels in-vitro. Human infragenicular VSMC isolated from diabetic patients undergoing lower extremity amputation were used. Cells were identified as VSMC by immunohistochemical analysis. Standard hemocytometry and 3H-thymidine incorporation quantified cell proliferation after incubation for 6 days and 24 hours, respectively. These data suggest that thiamin inhibits human infragenicular VSMC proliferation induced by high glucose and insulin.