Estimate which areas of the Trail you will be walking at night and practice them. Two weekends before the event try walking about 8-10 hours on both Saturday and Sunday. This helps to accustom yourself to walking when stiff and tired. Allow your muscles to relax during the following two weeks. For those who really want to complete the course with as little discomfort as possible, a short run every day will give additional basic strength and fitness.
Tired muscles start to stiffen during long rests.
Shorter, more frequent stops may be more advisable during the event. You are strongly advised to take out insurance to cover injury or death, and any damage to personal property.