GTM Isolation of DNA from Mouse Tail Biopsies 1. Hold mouse firmly at base of tail with one hand, with the other cut off 0.5 to 1.0 cm of the tail tip with a scalpel or single edge razor blade. Add 166.7 microliters 6M NaCl. Shake vigorously for 15 sec. Spool precipitated DNA with closed end capillary tube.
Volume depends empirically on pellet size.
Quantitate DNA and store at 4 degrees C until needed. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Research.