The section from Isinours Junction to Preston is a very scenic valley with abundant foliage and wildlife and follows the Root River. From Preston , the trail follows and crosses Watson Creek, the South Branch of the Root River and Camp Creek, passing through wooded areas and farmland eventually climbing out of the river valley and then cross country into Harmony . The trail is rich in scenery of rolling hills, farmland, and abundant wildlife. The Root River State Trail is a 43 mile multiple use trail that descends from Fountain into the Root River Valley through Lanesboro , Whalan, Peterson , Rushford and in to Houston . The trail provides outstanding views of the soaring limestone bluffs of the Root River Valley.
Wildlife is abundant and sightings of wild turkey, deer, hawks and turkey vultures are common.
Rattlesnakes are occasionally found on rock outcrops and in the river bottoms. These permits may be obtained through the County Courthouses, the Department of Natural Resource offices and from agents who sell game and fish licenses.