For virus resistant plants where the transgenes are derived from a viral genome, in addition to transgene protein analysis, determine transgene RNA levels in tissues consistent with the associated regulatory regions driving expression of the transgene. If the protein concentration is below the limits of detection, mRNA data may be substituted. Protein analysis for products of genes used only as selectable markers may be waived under certain circumstances, e.g. when there is at least one complete copy of a selectable marker gene present and the effective expression of the selectable marker gene is verified by the process used to select the transformed tissue. If the target protein levels are below levels of detection, determine target mRNA levels. When a fragment of a coding region designed to be expressed in a plant is detected, determine whether a fusion protein could be produced and in which tissues it may be located.
Sizes are approximations.