When this DNA is in one orientation, promoter is adjacent to coding region and transcription occurs. H segment is flanked by two inverted repeats that contain breakage sites used in recombination. H is not a transposon - it merely inverts and requires at least one host function because inversion in vitro is greatly stimulated by crude cell extracts that don't contain recombinase. Similar situation seen in phage Mu, where G region can be inverted. Cointegrate of conjugative and nonconjugative plasmid formed that is transferred to recipient and resolved.
Transposable element can initially be on either plasmid.
Mutagenesis to localize and characterize genes. Stable mutations but can't be directed. Impact of transposable element on public health by conferring antibiotic resistance. Change in DNA essential to evolution whereby organisms slowly change to create new individuals better able to exploit prevailing environmental conditions.