The process can be reiterated with downstream genes to define pathways. Clients can provide Sequitur with a gene sequence and we will design custom PCR primers and TaqMan probes for that gene using our sequence analysis system. Sequitur also offers a service to develop quantitative-PCR assays. After the appropriate assay conditions have been determined, quantification of experimental samples can be performed by the client or by Sequitur using our ABI 5700 system. View the Pricing page.
Some cell lines are notoriously difficult to transfect using standard vectors.
Sequitur's OmniVect transfection vector, in combination with our advanced transfection protocols, can often succeed where standard vectors fail. OmniVect also often leads to more uniform expression distribution. Test our luciferase control vector in your cell system. Serum stability allows for continuous transfection for up to 5 days. It is highly active in broad spectrum of cell types.