We want to include everyone, so if there are any technical glitches, we'll go to our back-up methods of connection. Stress goes up when participants are overwhelmed by a large number of people to interact with. For example, I am Mary from Fort Worth. Stress goes up when participants are aware of the typical 6 - 8 second delays in transmission. In general, the best advice is to simply slow down and let the audience see that you are not rushed.
Stress goes up when participants are self-conscious of their appearance on monitors.
Measure of monitor should equal the number of people. So, a 28-inch monitor can comfortably service 28 people in the room. Comfortably, just one. Mark the boundary with masking tape or simply a table or chair pushed into position. Make eye contact with the camera, or with a monitor if there is one directly below the camera.