Dip pairs of slides into prewarmed .2X SSC to separate and rinse them before placing in rack. SSC with gentle agitation 3. Repeat NTE wash 5 min 6. Use just enough to cover slides and place on rocking platform at room temp. Make a puddle of antibody solution in a plastic weigh dish.
Sandwich slides together and allow capillary action to pull up solution.
Drain on Kimwipe and repeat, try to avoid bubbles. Elevate slides above wet paper towels in plastic container and allow to sit at room temp for 2 hours. Drain slides on Kimwipes and separate. Place on bottom of plastic container as in step 11. Sandwich slides and draw up solution as in 13.