In our lab experiments are in progress to demonstrate several mechanisms of translational coupling and to determine more generally how coupling is used either to coordinate expression of two adjacent genes or to downregulate expression from one to the next. We are also exploring the principles that govern the relative levels at which in-frame overlapping genes are translated. Finally, we are beginning study of an extraordinarily efficient translational start site which appears to derive its strength from nonstandard elements and hence has the potential to reveal previously unrecognized interactions between mRNA and the ribosome. Emerging features of mRNA decay in bacteria. Appropriate expression of filamentous phage f1 DNA replication genes II and X requires RNase E-dependent processing and separate mRNAs.
Translation limits synthesis of an assembly-initiating coat protein of filamentous phage IKe.
Filamentous phage IKe mRNAs conserve form and function despite divergence in regulatory elements. Mutational analysis of an inherently defective translation initiation site.