Of course, the concentration depends on the cell line. Hybridoma lines can grow in the Cellmax TM for many months. The Cell Center has been able to grow a hybridoma line in the Cellmax TM for 3 months. The cryoprotectant allows the cells to be frozen safely, ensuring that they can be later thawed and grown. The cells can not be grown.
Samples can remain stored in liquid nitrogen for years without loss of viability.
The lab has separated over 6,000 blood samples. Over 200 fibroblast lines have been established at The Cell Center. The blood should be inverted several times to mix the preservative and blood. The likelihood of a good separation is low. Lymphocyte viability will be greatly lowered and cells may be lysed.