Multiple probes are pooled in a single hybridization, and positive colonies are re-arrayed for hybridization with individual probes. The program currently operates in semi-automated manner, with the potential for full automation in the near future. In the current mode, the user loads a phosphorimager file of hybridized filters and selects the filters to be analyzed. Using the positive control offset signals, the program dynamically draws gridlines to indicate the 384 major subdivisions on the filter. When a subgrid containing a positive is selected, the program displays an enlarged view of the positive subgrid with the 6 x 6 minor gridlines drawn.
True positives appear in unique duplicate patterns.
The program highlights the position of the expected duplicate signal when the user selects a positive, thus providing immediate feedback on the validity of a given pair of observed signals. The program is linked to the LLNL mapping database to facilitate entry of hybridization results into the database and retrieval from the database of map information relevant to positive clones identified.