DNA markers linked to white eye have been identified. One of these DNA markers has been cloned, sequenced, and physically positioned by in situ hybridization near the end of the long arm of sex chromosome X1. This suggest the gene controlling white eye and the gene controlling virulence to H13 are also on the long arm of sex chromosome X1. This will provide knowledge at the molecular level of how the insect overcomes resistance in wheat. Dissected alimentary tract and salivary glands of an Hessian fly larva.
Salivary glands are the long filamentous structures going up.
Large basal cells of the glands contain polytene chromosomes. In situ hybridization of cloned genomic DNA sequence to the polytene chromosomes of Hessian fly. Arrows indicate the location of the hybridization location of the hybridization. White and dark-eyed male and female Hessian flies. The white-eye trait in the insect is linked to virulence to resistance gene H13 and resistance gene H9 in wheat.