Purification and catalytic activities of the two domains of the allene oxide synthase-lipoxygenase fusion protein of the coral Plexaura homomalla. To investigate nuclear dynamics in yeast expressing altered levels of PLC1 activity. The nuclear migration and DNA segregation which are dependent upon cytoplasmic and intranuclear microtubules, respectively, will be characterized by microscopy. To characterize the genetic and physical interactions of PLC1 with the MAD1 and BBP1 proteins. Description Microtubules are important cellular structures that are involved in cell division.
Several chemicals can be detrimental to theses systems.
This project involves the study of microtubule toxicity in a yeast model. Benomyl is widely used as a fungicide and causes male reproductive damage in rats and mice. Testicular toxicity may result from the same mechanism by which the compound acts as a fungicide, the inhibition of microtubule polymerization. These observations suggest that PLC1 activity may regulate microtubule stability in yeast. To date this work supports the proposed hypothesis that PLC1 may regulate a microtubule repair pathway.