When the DNA strand has been completely digested, place it into a cup labeled with both enzymes. Running a gel with your pieces Complete the table below to indicate where students will be placing their fragment data. Obtained Student 1 Student 2 Students 1 and 2 Count the number of beads in each fragment, then multiple that number of beads by 1000. By multiplying by 1000, the fragment sizes can be placed onto a logarithmic scale to accurately express the relative distances one sees in electrophoretically separated DNA bands. Fill in an imaginary gel.
Students fill in a paper gel by shading in lines on a piece of logarithmic scale paper.
It is up to the teacher to emphasize those aspects of the fragment migration they wish to. Use logarithmic paper to simulate a gel, having students determine the appropriate distances needed to place their fragments on the paper. It is given in it's DNA and cDNA form. It has been altered slightly ot provide more than one restriction site for a few enzymes. A matrix is provided below that can be used to design restriction problems of varying degrees of complexity.