Make a quick evaluation of the number of cells occurring on the grids. There may be too many or too few. If so, start over with a different dilution. You should be able to count units in at least two dilutions to check accuracy and dilution procedures. Count units in both grid areas.
Units can refer to conidia, zoospores or sexual spores like oospores, ascospores, and basidiospores.
D. Calculations 1. Count the number of spores in several squares and obtain an average number of spores per square. Multiply by 10 to obtain number of spores per mm3. 5. Multiply by 1000 to convert mm3 to ml. Multiply by dilution factor to get spores per ml in stock.