One way to do this is to pour cell lysate over a MOP-GTPgammaS affinity column. Proteins that elute can be separated into fractions or purified from a native gel. Mix your GAP with MOP and GTP, and compare hydrolysis rate with MOP plus GAP alone by the same assay you used in 2a. 2 pts. for explaining that the ribosome is the GAP for EF-Tu, of which MOP is probably a homolog. Stresses increase the activity of kinases in order to down-regulate translation.
Translation is halted.
Some of these cells will contain a mutation in the target protein of the kinase. 3D. 7 pts. Several techniques will work. This is the best, most direct technique to see phosphorylation of one protein by another. If the other protein is bound to the column, you have identified a target of the kinase.