Tube is immediately returned to ice for 2-5 minutes 6. LB broth is added to both tubes using sterile techniques 7. Test tubes are placed in a 37 shaking water bath for 45 minutes 8. Pipette is used to add normal E. coli cells to plate A and transformed cells to plate B. Cells are spread on plates using a cell spreader that has been dipped in ethanol, flamed, and cooled 9. The next day, students observe and count colonies growing on each plate.
Students then create a graph to demonstrate results.
Students create a poster board that describes the experimental procedure and discusses what is happening in each step of the procedure. They will also demonstrate and explain their experimental results.