All antigens should be processed to minimize microbial contamination prior to mixing with adjuvant. Boosters should be given 14-28 days after primary immunization. Test bleeds should be done approximately 10-24 days after the boost. These are guidelines only. Absolute time frames should be dictated by the investigator's experience or pilot studies.
Several methods are available for blood collection.
For assistance and training, consult the LAR veterinarian. Should antibody titers not be high enough, multiple boosters may be administered. Animals with high test titers should be boostered 3-4 weeks after testing, then sacrificed 3 days later for tissue harvesting. For training or assistance, consult the LAR veterinarian. Further work for cell fusion and hybridoma selection is performed in vitro.