The perfusate flows transversely across both cut surfaces. The steady flow of warmed media across the slightly submerged cut surfaces of the slice is sufficient to keep many tissues alive and responsive for 10 or more hours. The fluid level is set to 1mm above the mesh by a dam at the end of the chamber. Fluid overflowing the dam is drained via a vacuum line to waste. This initiates a circular flow and oxygen enriched saline which continuously permeates the slices.
Slices remain viable for hours while awaiting transfer to an examining chamber.
The TC 202A allows the researcher to control the command temperature from alternative locations. Temperature can be controlled either from a thermistor place in the bath, or from a second thermistor permanently positioned on the plate containing the regulated surface of the Pelter devices. Rotating mount storage block for Vibratome price . Building on a tradition of Excellence, The Vibratome Company now offers a complete line of rodent brain matrices. The matrices are designed for easy free hand sectioning of discrete regions of the brain.