Through two weeks of numerous trials, I have learned a great deal about DNA extractions. I originally worked with eight different protocols to modify, simplify, and develop good extraction labs. My intent was to produce simple DNA extractions that use various types of cells. The materials used come from the grocery store, health food stores, and butcher shops.
Several extractions require a centrifuge.
I made it a point to get a centrifuge this year so that I could run the experiments. If you do not have access to a centrifuge, you might run the extractions as written and try to create ways to get around it. The centrifuge step can be skipped in the thymus experiment and good results still obtained. It's amazing what steps can be eliminated or modified. It is best to begin collecting the materials two weeks in advance.