Recoveries decrease for larger and smaller fragments as indicated on the gel. Gel purification of DNA fragments with different sizes Researchers purify DNA fragments covering a wide range of sizes. To test the effectiveness of gel isolations over a wide size range, we performed two tests. All the fragments were successfully recovered. Although fragments larger than 9kb may be extracted from Promega's Low Melting Point Agarose used together with Wizard PCR Preps, the yield decreases with larger size, and the linearized DNA can shear during the mechanical process.
Yields are dependent on fragment size.
Fragments with sizes between 1-4kb will be recovered more efficiently than fragments 6kb or larger or less then 0.5kb. Hin d III DNA fragments from Low Melting Point Agarose. Yields decreased for larger and smaller fragments as indicated on the gel. The two fragments from this double digest were then isolated from Low Melting Point Agarose, religated and transformed into E. coli . The results from this second experiment indicate that T4 DNA Ligase is not inhibited and that no contaminants are present which interfere with transformations.