T.C. Saido, C. Haass, E. Masliah, D.J. Selkoe, D. Games. Computerized image analysis was performed on whole coronal sections. The number of plaques detected by each antibody increased with age but remained a small subset of the those labeled with R1282. We are now examining the accrual of other inflammatory proteins within plaques and defining the plaque morphologies associated with each marker in these mice. Briefly ethanol-fixed brain sections from AD brain were run alongside mouse sections as positive control tissue for immunostaining.
Some preliminary data is presented.
L-Asp 1 is the earliest species deposited and the most abundant species at all ages. All other species appear in subsets of plaques and increase with age. IR was found in fewer plaques but, also increased with age. C3 detected a small subset of plaques in the brain tissue of the 8 month mice. The number of plaques increased with age, but remained of small subset of those reactive for R1282.