The role of Ty3 integrase in position specific transposition. The role of Ty3 integrase in position specific transposition. H. Nymark and S. Sandmeyer . University of California, Irvine Ty3 is a retroviruslike element of S. cerevisiae . Ty3 transposes into positions immediately upstream of RNA pol III transcribed genes.
Transposition is monitored using a genetic assay.
A screen was devised in order to identify proteins that potentially interact with the integration complex. Plasmids carrying the gene for either subunit were mutagenized and introduced to the original transposition assay in order to assess their effect on transposition of the IN mutants. Data on the progress of these experiments will be presented.