Departs from the terminus at Thomastown Railway Station and runs via Station Street, Alexander Avenue, Darebin Drive, Dalton Road, Burton Street, Edmondson Street, Curtain Avenue, Paschke Crescent and Station Street to Lalor Railway Station. It then runs via Station Street to Lalor Railway Station. It then runs via Station Street to the terminus at Thomastown Railway Station. Route 559 is timetabled to connect with trains to the City at Lalor Station and trains from the City at Thomastown Station. There is no service on Sundays or Public Holidays.
Times are subject to alteration without notice.