The RMS is 1.12 microns in the example shown. Curves for 53 traced nuclei positioned as they occur in the sample. Not all nuclei in the data set were traced. The scale is slightly different then in Figure 9. The orientations may be correlated to the differentiated state of these embryonic Drosophila cells.
Volume rendering Above the microscope data is shown as a constant intensity surface.
This 3D representation has advantages and disadvantages over flipping through the 2D stack of images. It shows the 3D geometry well, but you don't see as much detail in the shapes and intensities of the colored spots. A 3D volume model in Chimera gives an improved view of spot shape and intensity. Stereo pair showing volume rendering of FISH labelled Drosophila nucleus. The volume model is a projection of the 3D data set.