The subject matter can be enhanced and explained through the way you drawn and by what drawing media you choose. Crisp line, soft pencil, blurry pastel chalk, crumbly crayon, watercolors and felt tip markers all impart different feelings when in motion. Wet media, such as brush and ink, watercolors or felt tip markers make wet-looking images. The edges of each are different. Felt tip markers tend to bleed beyond your outline when new, and to leave dry, even lines when old.
Watercolors can make the paper wrinkle permanently.
On film, these particles make the image surface very active since each tracing will have a different particle arrangement. Pastels, charcoal and soft graphite will smear easily, so take care in storing and handling pages with these substances. The best way to find out what looks good in rotoscoping is to arm yourself with a variety of drawing tools and make a test. Copy a short sequence of film frames in as many different ways as you can think of, film your drawings and see what looks best. You'll discover that rotoscoping has a special airy or watery surface to it that comes from minute wiggles your hand makes as it traces around a shape.