When possible, use SCSI drives over EIDE or IDE drives. But do NOT split the file over different volume sets on the same drive. This will cause a degradation in performance and will cause excess wear on the drives. Use a defragmenting tool - It is possible to defragment disks without a tool, however, it can be just as tedious as defragmenting an MPE system without one. Install only the network protocols you use.
Unused protocols can use memory and take CPU time away from the one used.
If more than one protocol is used, bind them together. If you have mixed adapter speeds, consider upgrading the slower adapters. Performance slows when a fast adapter sends data to a slow one. Performance Tidbits NT uses some ambient CPU resource every second or two for System operation and pinging of network machines. NT makes great use of drive space by using it as virtual memory so the more drive capacity you can get, the better.