The two types differ only in 3 nucleotides. 2 Bender and Kleckner denatured both elements and reannealed them together in the same tube. Some element fully reformed, but others formed heteroduplexes. 3 Molecules were introduced into cells at a concentration low enough to ensure that no cell would receive more than one.
Transposition occurs from the l vector to the bacterial chromosome.
If no transposition occurred a colony would not form since the Tn10 element would not replicate and confer tetracycline resistance to dividing cells. 5 Colony phenotype indicates the type of mechanism by which Tn10 transposes. As a cell divided, each strand of the heteroduplex served as a template for DNA duplication. If transposition occurred by a replicative mechanism, only solid color colonies would arise. Note that many colonies were of solid color.