1.750 in distilled water, make up to 1000 ml. E. Merck 70-230 mesh Astm Brockman Activity I, Art 1077. Calcium Sulfate anhydrous fine. E. Merck 60-100 mesh Astm Art 12518. F. Merck No. 7734.
Sodium sulfate anhydrous.
Defatted cotton wool. Tap the columm between addition of the materials to ensure even packing. Then seal the column with alcohol lamp. Add 200 ml of extracting solvent and shake contents of the flask well by a shaker for 30 min. or blend with a medium speed blender for 3 min. Filter contents through a folded paper filter into a graduated cylinder.