It defeats the purpose of the introduction if you start out with a penis joke and then segue into a joke on airports. Try to make your opening funny. This seems obvious but is very important and often forgotten. You have one minute in your routine to get the audience on your side and laughing. Make your opening memorable and use it as a springboard to a successful show.
Visualization is VERY important.
Try to picture yourself walking onstage in a comedy club, taking the microphone from the stand, then not passing out. This is also very important. Now visualize yourself saying something funny and the audience laughing. Try not to do this on the Deerfoot 500. Another trick that seasoned comics use is the insult.