The flats are placed on a heated germination bench. Newspaper on top of the plat prevents cooling by evaporation. Newspaper is removed when seeds germinate. Spray inoculum on plants in cotyledon stage up to fifth leaf stage. If leaves become water soaked, leaf tissue on older plants may collapse and resistant plants appear susceptible.
Warmer temperatures can cause a resistant reaction on susceptible plants.
Plants are rated as susceptible or resistant 5-7 days after inoculation. The hypocotyl of susceptible plants is girdled or has sunken necrotic lesions. Resistant plants show no reaction or, rarely, a faint water soaked lesion. A sunken lesion develops on the cotyledon of susceptible plants. Resistant plants show no reaction or a glossy spot where inoculum was applied.