Mostly crepuscular, but may be active day or night. Pronghorns may migrate between summer and winter ranges. In spring, winter herds break up into smaller groups. Home ranges are extremely variable, depending on habitat, year, and location. During periods of extreme winter weather may restrict activity to very small areas.
Territories usually contain some water.
Females are attracted to territories for mating. Polygynous, mating from mid-September to early October. Gestation about 252 days. Twins generally born in spring, single births are less common. Young precocial, and weaned during rut.