In addition, elapsed tape time must be monitored closely so that tapes do not run out in the middle of a test. Identification of the individual mouse will be done by placement of a 3x5 card under the camera view immediately prior to each mouse's test. Experimenters will wear surgical type gloves for this handling. Placement on the maze is done by hand to ensure consistency and orientation. Specific Testing Procedures 1.
Testing is to be done in pairs with the dual mazes.
Mice are set up and transported from the holding area in home cage pairs. Walk the cage to the testing room and place it on the table adjacent to the plus maze. Testing begins as soon as possible. Stopping and starting the VCR between tests of individual mice is recommended to save time on the tapes. Place the 3x5 card under the camera view briefly to identify the mouse to be tested.