T here are presently several techniques employed by commercial and academic laboratories worldwide.
Select Breeders Service is equipped to utilize any of these various techniques in customizing a freezing protocol most suitable for the individual stallion. There are many differences between the techniques, however, a few basic principles are common to all. Following collection, the semen is diluted and centrifuged to remove seminal plasma, which can be detrimental to sperm during the freezing process. This process also concentrates the sperm so that a sufficient number of motile sperm can be included in a single insemination dose. After seminal plasma has been removed, the sperm pellet is re-suspended in an extender which contains ingredients to protect the fragile sperm during the freezing and thawing process.