Conditions of Capillary Electrophoresis Positive, negative, and neutral molecules can be separated.
Separation can take place in a fused silica capillary tube coated with polyamide to give strength.
The fused silica tube are 30 to 100 cm in length with an internal diameter of 25-100um. The hydroxyl group of the fused silica dissociates leaving the tube with a net negative charge.