Additional sources which are of use with restriction enzymes include a number of databases and sequence analysis software packages as well as vendor materials. This article summarizes some of these standard resources. Restriction endonuclease reviews Several excellent review articles that convey a wealth of information concerning the properties of restriction enzymes are available. These references cover the commercial availability of restriction enzymes, their general properties such as sequence recognition, star activity, methylation sensitivity, and basic procedures for use of restriction enzymes. The information presented in the Roberts and Macelis review is updated daily, and these updates are incorporated monthly.
Specialized versions are available.
This important reference contains an enzyme listing detailing the recognition sequence and the microorganism from which each enzyme is isolated. Additional information includes commercial sources of enzymes, any known isoschizomers or neoisoschizomers, and methylation sensitivities of the enzymes, if known. A separate table presents recently identified restriction enzymes and provides a published reference. For previously identified restriction enzymes, a published reference source may be found in earlier editions of this review. The Kessler and Manta reference contains information particularly useful for developing cloning strategies, presented in extensive tables.