In normal circumstances, lacto-glycerol should be used in preference to lactophenol. This removes most of the water. The nematodes are then in pure glycerol and are transferred to a drop of glycerol on a glass slide ready for mounting. Simple Evaporation method. 25 ml Partially cover with a glass plate or microscope slide for the first day, and gradually remove the cover over the next two days until the solution is fully exposed and nematodes are in pure glycerol.
Transfer nematodes to a drop of glycerol on a glass slide ready for mounting.
Slow glycerol evaporation method Transfer nematodes directly from fixative to a few mls. If the processing is done in an oven, the watch glass should be partially covered at first so as to slow down the evaporation process. When all the water has been removed the nematodes can be transferred to a drop of anhydrous glycerol on a glass slide ready for mounting. This can be done by keeping the glycerol for mounting in a desiccator. Mounting Arrange nematodes in the centre of the drop of mounting fluid, separated from each other and resting on the glass slide ie. not floating in the mountant.