DNA as replicative intermediate 3.No Okazaki Fragments found. Possibly type 1 and type 2 replication. Pre-terminal protein linked to 5' deoxy cytidine of the terminal inverted repeat through a phosphodiester bond of serine hydroxyl group and 5'hydroxy terminal of deoxycytidine residue. Proteolytic processing occurs during viral maturation and is a viral function. Replication in vitro requires three viral proteins, the DNA pol that forms a heterodimer with the pre-terminal protein.
Viral replication utilizes a viral encoded polymerase.
Site directed mutagenesis in this area destroys the ability to replicate. Thus Tp protein acts as primer for adenovirus replication. A host encoded non specific DNA binding protein stimulates the replication. This ssDBP also used for elongation of the DNA.Full length elongation displaces parental strands. Chain growth proceeds by base pair copying of the template strand until about half of the molecule is copied.