These are amphiphilic molecules that stabilize the drop by decreasing the interfacial energy. The inverted emulsion droplet sediments into the second water phase. It passes through an interface where the second layer of lipid is sitting, making the final vesicle. Confocal microscopy images of EggPC with rhodamine-PE vesicles in polarized light. Larger, not monodisperse vesicles obtained by extrusion through an 8um filter.
Some vesicles are out of focus.
The picture on the right shows a liposome encapsulating actin fluorescently label with rhodamine. After staining the red liposome are in the cell, and the yellow ones sit outside.