Our laboratory is focused on cellular mechanisms involved in electrolyte transport by proximal and distal renal tubules, and on components of the intrarenal mechanism responsible for feedback control of glomerular filtration rate. Experimental approaches include both in vivo microperfusion of surface segments, and in vitro perfusion of isolated renal tubules. Morphometric techniques are employed to assess structural consequences of chronic changes in transport function.
Transport is assessed by tracer flux measure ment and by electrophysiological methods.
Of particular relevance to current problems in clinical nephrology, the mechanism by which protein feeding reduces the responsiveness of the tubuloglomerular feedback mechanism is under investigation. Active potassium absorption by the renal distal tubule. Renal mechanism of trimethoprim-induced hyperkalemia. Yale Section of Nephrology. All rights reserved.