Samples can be analyzed if they have been fixed with paraformaldehyde, but such treatments can potentially alter the results. Prepration for Cell Sorting Planning ahead is essential. Wistar has only one cell sorter, and access is on a first come, first served basis. Sorting requires much more setup than analysis. Please discuss experiment thoroughly with operator before proceeding - it will save you time and confusion.
Viability is dependent upon cells and harder to predict.
Two sub-populations may be sorted simultaneously. These may be any populations of your choosing, as long as they are mutually exclusive. The flow cytometer's count of number of cells and their viability will probably be significantly lower than expected. Losses occur at every step in the process, which is why there is a correction factor of 0.5. Always bring more cells for sorting than you think are needed, if feasible .