They breed from May to July. They use permanent or semi permanent wetlands to breed. The males call from vegetation in the water. The call is the best way to distinguish this treefrog from the gray treefrog in the field. It sounds like a duck's quack with a trill to it.
Tadpoles have a red tipped tail, unique among Minnesota's tadpole's.
They are always bright green when they transfrom and will remain so for some time. Color change in adults varies depending on temperature, activity level, and color of surroundings. Adults migrate to summer feeding habitats after the mate. They may call periodically from their perches throughout the summer, especially in evening or after rains. They overwinter under shelters of bark, leaves, rocks or logs.