The orientation of this multiple cloning region differentiates this vector from the pGEM -1 Vector. Multiple Cloning Site and Circle Map Figure 1. Vector promoter and multiple cloning site sequence. The sequence shown corresponds to RNA synthesized by SP6 RNA polymerase and is complementary to RNA synthesized by T7 RNA polymerase. Vector circle map and sequence reference points.
Vectors are essentially identical except for the orientation of the multiple cloning site region.
Please note that we have not verified this information by restriction digestion with each enzyme listed. For more information on the cut sites of these enzymes, or if you identify a discrepancy, please contact your local Promega Branch or Distributor. Between 1 and 5 Times. The enzymes listed with asterisks are available from Promega. That Do Not Cut the pGEM -2 Vector.