For example, if being used for antibody production consider the method of peptide-carrier conjugation to be used prior to synthesis, design accordingly, and optimise the chances of subsequent success. All peptides, for whatever purpose, should be analysed both chromatographically and mass spectrometrically to assure a high degree of chemical integrity. Conjugation of peptides to carrier proteins is necessary in order to obtain a high level of immune response. A well designed and prepared conjugate is more likely to produce a desired result. Rabbits are often first choice for polyclonal antibody production but others should be considered.
Sheep have advantages for many users, chickens for others.
Peptide-reactive antisera are seldom the goal. Protein-reactive IgG antibodies arise and peak later in an immunisation schedule. By screening in an appropriate system for the desired reactivity, programs can be modified accordingly with consequent benefit. Consequently, small samples of each test bleed are routinely supplied for Client testing. Projects may then be terminated once the desired response is obtained Antibody purification A polyclonal antiserum should only be purified where it proves to be necessary.