The medium contained inorganic salts, glucose and yeast extract. A callus is an irregularly structured mass of cells whose rates of differentiation and growth differ.
Some parts stay meristematic while others sclerify.
Sclerification leads finally to the dying of the respective tissue part. Callus Explants from Root Pieces of Cymbidium ensifolium . The bar represents 2.5 mm. Such a culture can be kept forever by successive transplantations onto fresh nutrient agar. The transplantations occur every three to eight weeks.