Band resolution better with less complex samples. Not recommended for protein recovery. Band intensities are slightly less than with standard Coomassie stain. Very clear background. Detection sensitivities listed here were determined using minigels.
Sensitivities are typically somewhat lower in large-format gels.
The ChromaPhor Protein Visualization System was developed to allow direct detection of protein during electrophoresis in SDS-PAGE minigels. To use this system, the ChromaPhor stain is simply added to the upper gel reservoir buffer at the start of the run. If additional protein detection sensitivity is needed, staining can be enhanced to 100-200ng of protein per band by a very short incubation of the gel in mild acidic conditions. Because organic solvents are not used with the ChromaPhor System, the SDS coat is not stripped from the separated protein bands. Proteins stained with the ChromaPhor System are not chemically altered or fixed in the gel matrix.