Using Anti-sense Technology . Abstract Anti-sense technology provides a method of decreasing the expression of specific genes. The overall objective of this experiment is to create a tool that can be used in the manipulation of the large sub-unit of Rubisco, and to determine the adverse affects of photosynthesis in Chlamydomonas. Introduction Different technologies available have allowed scientists to decrease the expression of nuclear-encoded genes.
Technology is an authentic experiment .
If successful, it will be the first time anti-sense technology has been used to affect the expression of plastid encoded genes. Photosynthetic eukaryotes reduce CO 2 . Carbon fixation is catalyed by Rubisco in photosynthesis. Rubisco is an enzyme that fixes 200 billion tons of CO 2 annually. The large sub-units are encoded by the gene rbcl in the chloroplast genome and synthesized by the chloroplast ribosomes.