The target cells for HIV infection, either in the human body or in cell cultures, have molecules called receptors. The CD4 receptor and the CCR5 coreceptor are molecules which determine HIV-1 binding. After this binding or fusion event, the virus injects its genetic material into the cell, beginning the complex process of converting the target cell into a virus factory. Within 24-48 hours, envelope proteins, gp120 and gp41, are expressed on the surface of the infected cell. The TAD process starts by labeling or tagging infected target cells with monoclonal antibodies specific for gp120.
Uninfected cells pass through the column and are collected.
Removing the column from the magnetic field allows the collection of infected cells. Bio-Tech Imaging products are currently for Research Use Only and not for use in diagnostic procedures. Purified HIV is a patented product. BTI cannot grant customers any license, express or implied, to make, use, or sell it. A license for purified HIV is available from the National Institutes of Health or corporate licensees having a license to sell it.