Byproduct credits from oil or meal sales can lower production costs. Market size and product form drive the production of grain. High agronomic quality of production lines is positively correlated with yield. Therefore, parent seed for production lines is produced through a rigorous backcross program. This will allow parental lines that consistently produce large amounts of grain from which transgenic proteins can be extracted.
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Perhaps the most important issue in protein production from transgenic plants is that of expression level. Expression of foreign genes is a function of the strength of the promoter, the presence of introns, and the codon usage within the coding sequence, the stability of the mRNA molecule and the location of the protein within the cell. Additionally, the tissue from which the protein is purified can have a major impact on the stability, and consequently the amount, of recombinant protein - seed generally bearing more stable protein than green plant parts . We know that any or all of these issues can have an impact on protein expression levels. Industrial enzymes usually require high volumes and moderate to low purification.