The adhesive on the slide is polymerized by a UV flash, securely anchoring the still frozen section to the slide. When a cut section is mounted on a room temperature slide, the flow of the melting ice causes water-soluble components of the cell to be displaced by the flow. In the CryoJane Tape-Transfer process, sections are cut, transferred and tightly bonded to the microscope slide without ever being permitted to melt. Slow freezing produces large ice-crystals which damage insoluble structural elements and cause displacement of water soluble components. In the CryoJane process the tissue is snap-frozen to minimize ice crystal size so the image quality is excellent for optical microscopy.
Thin sections help clarify structural relationships and reveal greater morphologic detail.
The bond between section and slide is resistant to proteases, alkali and acids. Cutting the Section 3. Transfer to Slide 4. Curing the Adhesive Coating 5. Removal ofTape 6.