Store lyophilized material at 4 DEG C. Store reconstituted material at 4 DEG C for immediate use. DEG C. Avoid frequent freeze thaw cycles. Human fascin is a 55 to 58kda actin- bundling protein, the actin binding ability being regulated by phosphorylation. In non-neoplastic tissues, immunohistochemical detection of fascin is predominantly restricted to dendritic cells and in the thymus was observed only in medullary dendritic cells. In reactive nodes, interdigitating reticulum cells of T cell zones, cells in subscapular areas, and cells of the reticular network are reactive.
Variable reactivity is sen in follicular dendritic cells.
Endothelial cells of all tissues exhibit variable reactivity. Lymphoid cells, myeloid cells and plasma cells are all unreactive. However, in cases of Hodgkins disease, including nodular sclerosis, mixed cellularity lymphocyte depletion and unclassified cases, most or all Reed Sternberg cells are positive for fascin. The staining profile for fascin may indicate dendritic cell derivation eg interdigitating reticulum cells, may evolve into neoplastic cells of Hodgkin's disease. This antibody may be a useful marker for the staining of Reed Sternberg cells in cases of Hodgkins disease and highlight the localization of fascin in other cell types.