Normal tissue embedding procedures involve aqueous chemical fixation, dehydration and embedding in hydrophobic resin. Results can be the possible loss of hydrophilic and lipophilic substances. Melamine resins, a new class of water-soluble embedding media for electron microscopy. Buying in smaller amounts is less expensive and may result in using fresher chemicals. The unique advantage to this resin is that the catalyst is supplied separate from the resin components.
Shelf life is improved and heat induced changes during shipping are eliminated.
Unique cutting properties have been attributed to this resin formulation. It is felt that proteins, nucleic acids and macromolecules are revealed at the surface during sectioning. Mixing of the initiator is required prior to use. It is a pale yellow liquid with a pungent odor that can be cured with either heat or UV radiation. Best results are attained when the resin is microwave cured.