Interpretation of the resulting highly complex data presents a novel challenge to molecular biologists. Development of microarray technologies. Microarrays have also been used to localize DNA deletions and amplifications. Transcriptional dynamics of complex transitions. Growing cells cycle through overlapping stages of responses to internal and external cues.
Some responses require concerted switching from the cycling routine.
We have shown that microarray analysis of mRNA levels can be very useful in delineating such responses by exposing transient changes in the RNA abundance, as well as changes accompanying new steady-states. However, even for well-established processes the exact sequence of transcriptional events accompanying the process is not known. Such sequences can be extracted doing high density fast-stop timecourses with appropriately defined references. I will also apply this approach to analyze Saccharomyces cerevisiae spore germination, one of very few physiologically relevant transitions regarding which we have absolutely no understanding. Bacterial chromosome dynamics and organization.