A syringe may be used to inject a suspension into the stem. Inoculum is made by combining on plate of Rhizoc growing on PDA with 100ml of water for 30 seconds in the blender. Inoculum should be injected until it begins to ooze out of the wound. Symptoms may include necrotic lesions and cracking of the stem. A soil drench may be applied by placing 1 ml of inoculum at the base of the stem.
Symptoms are necrotic lesions and cracking of the stem.
This method is similar to placing an agar disk on the hypocotyl, but it is probably a lot easier. Each flat should be divide into two halves with a piece of wood. In the center of each half flat place 25 oat grains and cover with soil. A template is used to punch twenty spaced holes in the soil mix. Plant one seed in each hole.