It was found to be an immunosuppressant. Smooth muscle relaxation has gained these drugs a reputation as enhancing sexual orgasm, especially among homosexuals. These drugs increase ocular pressure and can be dangerous for those with glaucoma. As with other inhalants, duration of the drug is short and it is difficult to identify intoxication. Nausea, cold sweat, irregular heart beat and increased heart rate can occur.
Some regular users develop a rash around the nose and mouth with a distinctive bluish tinge.
The drug has distinctive odor and the aroma of it will sometimes develop in a room when used. Headache can also be a sign of use, or occur after use, but this symptom dissipates as tolerance develops. There is a flushing of the face, neck, and upper body indicating increased blood flow. Nasal irritation can also be a sign of use of isobutyl nitrites. Nitrite poisoning can occur with repeated use and lead to anemia.