Lecture 5 Lecture 5 Polyclonal and monoclonal Abs Outline 1.
Secondary response is due to the response of clonally expanded memory B cells.
With each division the progeny of the fused cells lose chromosomes from one or both parent cells until the fused cell stabilizes. HAT medium - hypoxanthine, aminopterine, thymidine. De novo and the salvage pathway for nucleotide synthesis. Aminopterine inhibits the de novo pathway. Unfused myeloma cells die in HAT medium.