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Vectors are stable even when the DNA is subjected to freezing and heating.
The PCR process is covered by patents issued and applicable in certain countries. Promega does not encourage or support the unauthorized or unlicensed use of the PCR process. 4,766,072 has been issued to Promega Corporation for transcription vectors having two different bacteriophage RNA polymerase promoter sequences separated by a series of unique restriction sites into which foreign DNA can be inserted. No. 5,487,993 and European Pat. The single 3 -T overhangs at the insertion site greatly improve PCR product ligation efficiency by providing complementary ends to those of the insert.