Inoculate the tube to the bottom and gently rotate between the palms of your hands to disperse the organism. Do not shake or oxygen will be added to the medium. Incubate the tubes for 48 hours. Record the pattern of growth. Place the plate in the anaerobic jar.
Water is added with a pipette.
The jar is sealed and placed in an incubator for 48 hours. The indicator should be checked to make certain that all oxygen has been removed. A second plate should be placed in the incubator as an aerobic control. Observe for growth. Streak the organism on the appropriate agar medium 2.