This document is available in the Adobe Acrobat PDF Format. The soil extracts are washed away after incubation, and the PCB conjugate is added to the tubes.
Unoccupied antibody binding sites bind the PCB conjugate.
Excess PCB conjugate is washed away. An enzyme substrate and a coloring agent are then added. Reported detection limits are 0.1 ppb in water and 0.1 ppm in soil. The test kit was used to screen and quantify PCB contamination in soils at a solvent extraction system demonstration in Washburn, Maine. Highly contaminated soils were easily identified, and quantitative tests provided correlation to contaminant levels obtained by off-site laboratory analyses using EPA Method 8080.