Lateral roots 3 or more feet deep spread from a fibrous taproot. Aerial shoots are sent up at 2 to 6 inch intervals. Basal leaves are produced the first year, flowering stems the next. Pollination is mostly by honeybees, and wind pollination is limited. Most seeds germinate within one year.
Some seeds immediately produce rosettes before winter and emerge to flower the next spring.
Seeds remain viable in soil up to 20 years in some cases. As frequency of Canada thistle increases at a site, species diversity decreases, possibly due to allelopathic substances. Prairies, barrens, savannas, and glades are susceptible, particularly those sites that have been disturbed and are reverting naturally to native species, as well as those undergoing manipulative restoration management. As such, all landowners are required to control the plant if it is growing on their property. Control is considered to be prevention of seed production.