We are utilizing the genetic approach in order to map out differences in signaling mechanisms. Techniques include the use of cell culture, DNA preparations, transfection of cells, Western, Northern and Southern blotting, as well as protein kinase assays. Students should have taken a laboratory course, preferably analytic chemistry. A lab course with biochemical and molecular biology techniques would be advantageous for the student as well. Taking Biochemistry and or Cell and Molecular Biology would improve the students appreciation of the laboratory experience.
Students can receive credit and voluntary work is also available.
The molecular basis of differential gene expression among tissues and during development. Iron metabolism in erythroid cells, the GI tract, neurons and other tissues. This means that this rat has lost the function of the main iron transporter in the GI tract and the transporter responsible for most iron flux within cells. The microcytic mouse has an identical mutation. The hemoglobin deficit mouse has a mutation in a different gene that also affects iron metabolism.