Then adding 1 volume of 2X agar to l volume of 2X broth gives you 2 volumes of 1X medium. Thus, Kit A will gel 60 each 15 by 100 petri plates or 500 each 13 x 100 tubes. or any combination. It is easy to find foods in the kitchen to make media for bacteria, fungi, and yeast experiments. Both are superb media for many bacteria and fungi from fruit rots, plants, and soil. Soak hamburger or ground beef liver in water overnight or for a few hours and then cook and the broth or the complete medium with particles makes a superb medium for most bacteria.
Skim milk is a good medium.
The agar will gel 1000 mL of broths that you have made. TGY is a complete medium which will support the growth of most common bacteria. TGY contains 1 gram of glucose per liter. You may add other sugars for fermentation studies. TGY is a complete medium which will support the growth of most common bacteria.