Place fruits on inoculated soil in flats 8. Grow large quantities of oats. When plants reach tip-over, lightly rake two feet on each side of the row to help the oats stay in place. PDA disks placed in the center of a leaf will cause symptoms if humidity is kept high. Disks should be between 3 and 7 days old, and can be secured to the leaf by smashing the disk with your thumb.
Symptoms are necrotic lesions originating from the agar disk.
PDA disks placed on the hypocotyl at the soil line. Disks are the same as those used for the leaf method. Symptoms are cracking of the stem and brown lesions on the stem. Inoculum is made by combining on plate of Rhizoc growing on PDA with 100ml of water for 30 seconds in the blender. This is then poured in a beaker with one drop of spreader sticker.