Pour gels from top between glass plates. Insert comb, teeth-side down, into top of gel. Allow gel to polymerize. Remove gel from bag for immediate use or heat seal top of bag for storage. Running the Gel Use polyacrylamide gels cast from the Joey Gel Casting System above.
Slide clamps over plates and finger-tighten knobs to form seal between gasket and plate.
Repeat for second get, or clamp two blank glass plates onto other side if running only one gel. Add buffer to LBC to about 2-3mm above base of gel. Add buffer to UBC to about 5mm from top of gel assemblies. Put lid on. Prime the system by turning on water enough to circulate through system.