The collected urine was centrifuged at 5,000 rpm for 10 min at 4 C, or passed through a 0.45 m m filter. For single-cell calcium imaging, the cell was continuously washed out with the BSS solution. Dose response of the putative pheromone receptor. Recombinant adenovirus-infected VNO cells were stimulated by male mouse urine of various concentrations. Mouse urine was diluted with 1x BSS solution.
White arrows indicate the timing of stimulation.