3 pts. for an experiment that would isolate an interacting protein. One way to do this is to pour cell lysate over a MOP-GTPgammaS affinity column. Proteins that elute can be separated into fractions or purified from a native gel. Mix your GAP with MOP and GTP, and compare hydrolysis rate with MOP plus GAP alone by the same assay you used in 2a. 2 pts. for explaining that the ribosome is the GAP for EF-Tu, of which MOP is probably a homolog.
Stresses increase the activity of kinases in order to down-regulate translation.
Translation is halted. Some of these cells will contain a mutation in the target protein of the kinase. 3D. 7 pts. Several techniques will work. This is the best, most direct technique to see phosphorylation of one protein by another.