The purified calreticulin will be used as an antigen for polyclonal antibody production. Available Information and Reagents 1. A 1.4 kb Arabidopsis cDNA has been obtained from the Resource Center and its complete DNA sequence was determined. It was found to encode a 425 amino acid protein with high amino acid homology to human calreticulin. Biochemical supplies are available for purifying proteins containing a polyhistidine sequence from bacterial cell extracts using a metal chelation chromatography kit from Qiagen .
Resources are available for polyclonal antibody production in rabbits using purified antigen.
The plant biologist has expertise in plant immunocytochemistry and biochemistry. The T7 RNA Polymerase is a viral enzyme that only recognizes its own promoter sequence. First, it is a very high activity enzyme and is efficient at producing large amounts of RNA that is then translated into protein. Briefly describe the primary research objective of each of the 4 steps in the diagram. Immune reaction in the rabbit to produce anti-calreticulin antibodies.