In effect a sampled volume image is collapsed into a plane. Features in the volume data set are now in focus and in one plane. Maximum projection images may also be generated with tiffsplit4.exe or the LeicaTCS-NT software.
Maximum projections find the brightest points in the image stack.
This process can work amazingly well, especially for fainter structures such a thin dendrites. Tiffsplit4.exe is an enhanced version of tiffsplit.exe. Tiffsplit4.exe can also extract the image planes of multipaged tiff files into individual tiff or bitmap format files, which can be opened in general purpose imaging software, such as Adobe Photoshop. For a summary of tiffsplit4.exe command line switches execute tiffsplit4 without any command line options. All rights reserved.