RuO 4 staining also has a stabilizing effect against electron beam damage of material films. Ruthenium tetroxide penetrates tissue very slowly, reacting strongly with proteins, glycogen, and monosaccharides. Ruthenium tetroxide is a strong oxidizing agent and must be stored in the refrigerator away from direct sunlight. It reacts violently with filter paper and alcohol. Do not place ruthenium tetroxide solutions into waste containers containing alcohol, ether benzene, pyridine, or other organic compounds.
Ruthenium tetroxide has an acrid odor.
The vapors are irritating to eyes and the respiratory tract. Wear protective goggles and gloves and handle only in a fume hood. In case of spillage, flush with sodium bisulfite solution to decompose RuO 4 and then flush with plenty of water.