Preparing Your Samples for Mycoplasma Testing Cells should be grown for at least 3 days without antibiotics. Media should not be changed prior to testing. For monolayers, scrape cells and provide the cell suspension in a 5ml amount. For suspensions, 5mls is sufficient. Samples are accepted the first week of every month.
Testing is done the second week of every month.
Results will be sent out 2 weeks later. If the result is positive the researcher will be notified immediately. The core will clean up cells that are contaminated with mycoplasma. This is a lengthy process, which involves culturing the cells in specific antibiotics. Cells will be grown with and without antibiotics for several weeks and tested regularly.