An acid acts to release calcium from its combination with these anions and effects an ion exchange to give a soluble calcium salt. The calcium ions are effectively removed from the bone and remain in the decalcification solution. Specimens should be well fixed prior to RDO decalcification. Wash specimens thoroughly in tap water to remove as much fixative as possible. Place specimens in RDO and check periodically for complete decalcification by bending to check flexibility.
Process specimens with routine workload.
RDO as a whole is considered a non-hazardous and biodegradable material and may be disposed of down regular city sewers with a water flush according to federal, state, and local regulations. When architectural features of both bone and marrow are important in diagnosis, aspirated bone marrow is usually obtained with sternal puncture needle. Smears are made and the remainder is processed as a biopsy specimen. Wear gloves and protective clothing during decalcification procedures. Perform procedures under a fume hood.