By manipulating the solvent we were able to see how the carbonyl groups are affected and thus we will be able to assign a correct structure to our peptide. The solvent used is CDCl 3 and diethylamine will be used as our hydrogen-bonding solvent. This lends strong support to the validity of this new method. This method will then in turn be used upon bigger and more complex peptides. Understanding the conformational structure and how they fold in different solutions, and from other physical forces is intricate to be able to further understand how they fully work.
Proteins are extremely large molecules with large groupings of amino acids.
To just jump in and try to understand the chemistry involving one is near impossible. The work done in this research is focused on peptides which are a much smaller groupings of amino acids. In order to prove this we used 13 C NMR spectroscopy to view our carbon atoms. Each carbon will give rise to a single resonance. Where each carbon resonance lines up on the spectrum is determined by what type of groups are attached to it.