All reagents are provided, ready to use. The test most often used to demonstrate the accuracy of an assay is the assessment of recovery. Calibration depends upon the preparation of an analyte-free base matrix to which known concentrations of analyte are added. Recovery assesses the calibration of the assay and the influence of differences between sample and calibrator matrices. Dilution experiments are often referred to as parallelism studies because effectively one is judging whether or not dilutions of sample parallel the calibration curve.
Parallelism is assessed by assaying samples diluted in an appropriate analyte-free matrix.
Incubate 2 hrs. Aspirate and wash. Incubate 1 hr at room temperature. Aspirate and wash. Incubate 30 min.