Specifically, the effect of N-glycosylation on sodium channel gating will be studied. We have shown that as the amount of N-glysosylation of the channel increases, channel gating requires an increased depolarization. Interestingly, different sodium channel isoforms are expressed at different times and in different cell types, each with a unique glycosylation pattern. We will test the relevance of these differing glycosylation patterns to function by first expressing several different sodium channel isoforms in a single cell line comparing the extent and effect of each channel's glycosylation to its function. Then, chimeras will be constructed that test the relevance of specific glycosylation sites to channel function.
Many neurotramsmitter transporters are the sites of action of drugs of abuse and of therapy.
We will study how these proteins work, and determine how drugs such as cocaine, amphetamine, and Prozac alter transport activity. S.S. Tinkle, A.G. Koszowski, and S.R. Levinson. J. Gen. Physiol. E. Bennett , and G.A. Kimmich. Evidence for several potential dependent steps along the transport cycle .