We recommend not using them for high quality DNA sequencing. Provides very high quality DNA sequence. This provides high quality for DNA sequencing because the DNA turns out to be very clean. Contaminating agarose is believed to interfere with quality DNA sequencing. For the direct DNA sequencing of DNA fragments larger than 2.5 kb we recommend using nested primers.
Nested primers are more efficient for DNA sequencing than the same primers used for amplification.
The nested primers only need to be 30-50 bases instream of the amplifying primers. Back to the Top 5. Primer walking projects. The user is expected to analyze output chromatograms, design primers and supply those primers to the Facility for further sequencing. If you require primer synthesis please complete the primer order form .