Users interactively surround cells of interest using a mouse.
In version 2, ISAP permits a choice of methods for smoothing, thresholding, and edge detection. ISAP thresholds the enclosed image, either manually by preselecting a grayvalue threshold or percentage threshold, or automatically according to an algorithm published previously by the authors. Cytologic measurements are saved in an external database that can be uploaded into commercially available spreadsheet applications for statistical analysis of cell populations. Windows v. 3.0 or higher, and 256-color display. To test the robustness of ISAP algorithms, we performed image analysis on 20 normal intermediate cell nuclei at 40x magnification from Papanicolaou-stained cytology smears of the uterine cervix and vagina.