The approach is to be used when experimental errors are high for new sequences. It is shown that the performance is still very good when the nucleotide sequence of a related mammalian protein is available. Inputs for the two versions are slightly different. Genomic nucleotide sequence . The sequence can be named for reporting purpose.
Protein translation is performed according to the standard translation table.
The gene structure can be specified as pairs of numbers indicating exons. Characters other than digits are ignored. The agreement between the predicted and true gene structures is computed using this information. Target sequence . Selection of the target sequence is left to the user.