The actual cut site is generally within the recognition sequence but may be adjacent to or only nearby. Sometimes two different enzymes will recognize the same recognition site, they are known a isoschizomers. An enzyme which cuts DNA at a specific site called the recognition site. They are endonucleases in that they cut within the DNA rather than cutting at the ends. There are three types of restriction endonucleases, the most useful are the type II endonucleases.
Most recognition sites are palidromic.
Polymorphism A technique which detects polymorphisms between two people, such as single base changes or different sized minisatellites. The common feature is that when digested with a restriction endonuclease, DNA products are generated which are different lengths. STR Short Tandem Repeat Areas of repetitive DNA within the genome where the repeating unit is very small, usually 1-6 nucleotides. These are generally polymorphic within a population and can be used for bone marrow transplant engraftment, forensics, identity testing, paternity testing, etc. All rights reserved.