Victor Hatcher and Ruth Hogue Angeletti Albert Einstein College of Medicine.
Sample preparation is often the most challenging part of protein sequencing.
In the face of unpalatable sequence data presented by core facilities, many users give up the project in frustration, or try core facilities at other institutions, usually with similar lack of success. The work summarized here represents a successful resolution of a difficult sequencing problem by an unusually persistent investigator. This lab was trying to sequence several proteins isolated from subcellular fractions of cultured cells. The electrophoretic separations and stained PVDF membranes had an excellent appearance, and did yield sequence data. The original cell cultures required serum- containing medium for growth.