Five small disposable tubes. 10 ml serological pipette. This assay can be used with samples collected by passive drool, plain cotton Salivettes, cotton ropes, or after stimulating saliva flow using Trident Sugarless Gum. Freeze all saliva samples prior to assay in order to precipitate mucins. Pipette clear sample into appropriate wells.
Procedure Bring all reagents to room temperature except concentrated enzyme conjugate.
Keep this at 2-8 C until needed. Here is a suggested layout. Label four microcentrifuge tubes or other small tubes 2 through 5. Pipette 100 l of assay diluent in tubes 2 through 5. After changing pipette tips, remove 50 l from tube 2 to tube 3.