Carrier effect can be prevented by priming animals separately with carrier proteins. Antigen-antibody complex 3. Antigen enter LN thru 1. Afferent lymphatic vessels 2. Activated B-cells and some TH cells migrate from foci to primary Follicles 6.
Primary follicles develop into secondary follicles.
In germinal center, B-cells actively proliferate. Larger Size Expanded cytoplasm Diffuse Chromatin Lack of Ig. Express mIg 10. Centrocytes move from Dark zones into a region rich in follicular dendriatic cells and form Light zones. 1. expressing low affinity Ig or fail to recognize antigen on Follicular dendriatic cells die via apoptosis 2.