K d , so K d can be found if n can be. Otherwise shape very similar to single binding site. Final value again gives n if it can be reached, Shape otherwise the same as the curve for a single binding site. Final value again gives n if it can be reached, Shape otherwise the same as the curve for a single binding site. Overall, binding is inditinguishable from binding to four times the concentration of molecules with one binding site.
Subsequent ligands bind much more strongly than the first.
Linear plot shows only small amounts of intermediaries, sigmoidal Y curve. Scatchard plot has no intercept. The slope is the Hill coefficient or cooperativity Haemoglobin binding oxygen Shows a non-integral cooperativity, about 2.8. See Assignment 2 for an explanation. Oxygen concentration, and hence K d , are usually expressed as partial pressures.