Easy test to perform, for use in a lab. Lowest price per sample. Sensitivity of this test for some antibiotics may not be as good as the tests used at milk plants. The antibiotic in the milk sample competes with an enzyme tracer for antibody binding sites. A substrate then reacts with the enzyme tracer and produces a color change.
Samples containing antibiotics bind to the antibody and prevents a color change.
Easy to perform, for use in lab. Need other equipment from a well stocked lab. Results within 15 minutes. The well is pre-spotted separately with a calibrated level of antibody for the drug and a positive control. When a mixture of the milk sample and enzyme-conjugated substance is applied to the membrane, the sample competes for the antibody binding sites.