Only two-point linkage tests were possible, so the orientation on the chromosome with respect to the marker could not be determined. Tests with aneuploid stocks deficient for the short arm of chromosome 12 placed the gene in the long arm of chromosome 12. Knowledge of the location of the inserted gene would provide the opportunity to develop germplasm and strategies to maximize the efficiency of developing improved germplasm.
Transformation is considered to be a random event, with each inserted gene at a unique location.
So, each transgenic germline is the result of a separate insertion event, and the location of the insertion in the genome could represent different degrees of success in attempts at backcross improvement, depending on associated linkages. The location of transgenes inserted into a genome are important in genetic studies and breeding programs. Two separate germlines that exhibited monogenic dominance for resistance to 2,4-D were selected for linkage analysis. Multiple marker lines T582 and T586 were crossed with the 2,4-D resistant lines. J.E. Quisenberry, U.S. Pacific Basin Agric.