The choice of microplate will affect the performance of the test and allow the user to select the most appropriate procedure for their needs. The test requires a microplate luminometer to measure the light emission. It is also possible to use the kits with cuvette based luminometers and microplate Beta-counters. The assay can be fully automated using a microplate luminometer with reagent injection facilities. In any luminescent microplate assay, the microplate has a significant impact on the data.
Maximum sensitivity is achieved in completely white, high quality plates.
However, these plates are generally not suitable for cell culture as the cells cannot be observed during the culture period. In this case, plates with white walled wells and clear bottoms are available and are recommended. The optical properties for luminescence are slightly reduced in comparison to the completely white plates, therefore some sensitivity may be lost. Description Product Number ViaLight HS - High Sensitivity kit. Unless otherwise stated the following specifications are appropriate to both the ViaLight HS and HT kits.