Opening one clamp at a time. 10 micP3141 Opening Kleinert clamp. 17 micP3148 Macro instruments. 18 micP3149 Grasping needle by touching needle tip to background material. Needle passed perpendicular to the surface, about one needle diameter back from edge.
Tip passes between the jaws of supporting forceps.
Needle is delicately pulled through. Surface of left side of the slit is grasped with tip of forceps and elevated. 49 micG3048 The right-hand instrument is advanced through the loop and grasps the tip of the short end. The free end is pulled through, creating the second throw. The throw is tightened keeping both sides of the loop loose and equal so that the loop remains round as it comes down.