The use of yeast, animals and plants are discussed including the manner and means of creating transgenic organisms, the vectors employed and the associated selection systems. The Scientific and commercial benefits are described with transgenic animal and plant examples and a discussion of medical benefits emphasizing immunological aspects of recombinant DNA techniques. PCR and associated techniques and strategies, e.g. differential display versus hybrid selection. If required notes for lectures 1 -3 may be copied from the file available from RJS office. Vectors, transformation and selection techniques for yeast and animal cells.
Transgenic animals stategies for producing and selecting transgenic animals.
Application of recombinant DNA technology to medical diagnosis. List advantages and disadvantages of techniques and strategies in recombinant DNA technologies. Construct at an elementary theoretical level strategies for isolating and analysing genes. List and describe the attributes of animal, plant, yeast and insect vectors and the means of introducing DNA into their cells. Describe the processes used to construct transgenic animals and plants.