Screening for clones that are antibiotic resistant. It means that these clones carry the human gene. By using reverse transcriptase. This is the exception to the Central Dogma of Biology which states that the gene product or protein is translated from the message on the RNA, which was copied from the DNA or gene. DNA copied from RNA is the reverse of what you've learned so far, thus the name reverse transcriptase.
Viruses use reverse transcriptase.
DNA polymerase, the enzyme that catalyzes DNA synthesis, is added. DNA polymerase synthesizes the new strands on the two templates, so that at the end of the first cycle, the number of DNA strands is doubled. In the second cycle, the two double-stranded DNA are boiled again, unzipped, new DNA is synthesized on the templates, and by the end of the second cycle, four DNA copies would have been produced, and so on and so forth. Read the reports to learn more about the application of recombinant DNA technology in the food industry. There are studies showing a decrease in the population of pollinators such as birds, bees and other insects.