West Indian manatee. Bald eagle, eastern indigo snake, osprey, scrub jay. Numerous wading birds, including herons, egrets, ibis, and limpkins, are regular residents. Alligators and turtles are also abundant. Deer and turkey inhabit the uplands.
Raccoons are plentiful.
The 2,200-acre Lake Woodruff is located at the center of the Refuge. More than 5,000 acres of hardwood swamps form the transition between marsh and upland. There are 1,200 acres of upland at Lake Woodruff Refuge, primarily pine flatwoods. During the cooler months of late fall through early spring bird watching is at its peak as many migratory birds visit the Refuge. The Public Use Area is located at the end of Mud Lake Road, approximately one mile west of the Refuge headquarters.