Activation-dependent antibody staining may be affected by fixation and choice of anticoagulant. When performing assays to detect in vivo platelet activation, it is critical that stimulation of the platelets be minimized during blood collection or sample preparation. It is also important that processing time be minimized to prevent in vitro activation of the platelets. This whole blood method allows for minimal sample manipulation, and fixation of the blood with paraformaldehyde prior to staining inhibits spontaneous platelet activation. The PBS should be filtered through a 0.2- m filter prior to use.
Sodium azide is harmful if swallowed.
Keep out of reach of children. Keep away from food, drink, and animal feedingstuff. Wear suitable protective clothing. If swallowed, seek medical advice immediately and show this container or label. Contact with acids liberates very toxic gas.