When clean-up is complete, remove your outer pair of gloves while still under the biosafety cabinet, then autoclave the pan and bag. The tops must be closed and the outer surface of the tubes should be sprayed with appropriate disinfectant before removing them from the biosafety cabinet. These specimens must be unwrapped and worked on within the biosafety cabinet. Let sit for 20 minutes. Pour down sink drain.
Solid waste is put into an autoclave bag and autoclaved.
Hazardous chemical waste will be handled by Environmental Health and Safety. Autoclave bag must be put into a pan for autoclaving. Water must be added to the autoclave bag. A sterilization indicator strip must be included in each autoclave run. Perform a spore challenge in the autoclave on a weekly basis.