Do not include discussion, rationale, excuses, etc. Describe methods used for statistical analysis. Do not include in the materials and methods why you conducted the study, how it turned out, how you interpreted the results, or the rationale. All of these should be in the results and discussion section. The only purpose for the Materials and Methods is to provide enough information so that the experiments could be reproduced by a competent colleague .
Most authors choose to write the results and discussion together in a single section.
The Results should contain two goals. Second, you should present the data. The presentation of the data is important because you must and unambiguously illustrate your results before you can effectively discuss and present your conclusions. As a general rule, present the results that best clarify the important points of your discussion. If you made only a few determinations of your results, give the results in the text.