DEP-treat water, autoclave and use for making up solutions. Wear plastic gloves and use flamed forcepts to touch lips of tubes or pick up miracloth, etc. When cool add 5 mM thiourea 0.038 g 1 mM aurintricarboxylic acid 0.0422g 10 mM dithiotreitol 0.1542g 2. Adjust volume if necessary. Upon cooling the solution becomes a glue-like mass.
Measure amounts by weight.
Grind tissue with dry ice in coffee grinder. Transfer powder to a sterile, new 50-ml polypropylene orange cap tube with volume marks. When dry ice has evaporated but tissue is still frozen add 20 ml extraction buffer. Place on dry ice or in -80 freezer to freeze quickly. Vortex vigorously and continue thawing.