We have planned to sequence the ends in near future. The order is based on the Ensembl database. The clones are linked to their specific page. For old clones, not fully sequenced, the position on the Golden Path is derived from their STS content or end sequence. When gaps between two contigs are filled the contigs are merged in a new contig wich retain the name of one of the previous contigs.
Some regions are very rich in clones compared to others.
Oligos from the sequence of a specific region can be designed to amplify DNA stretches of appropriate length by long-PCR experiments suitable for FISH experiments multiple. The use of repeat-masked sequences can help in avoiding long repeats that would poorly contributed to the FISH signal. In our hands cloned PCR products are more efficient in FISH experiments than amplified products. To be quick, we just prepare the stab and put it on the mail. You may need to keep it at 37C for some hours.