Thus, it can be useful for sequential fertilization of small batches of eggs or for procedures where manipulations of eggs prior to fertilization are desired. Various batches of ovarian fluid have varying abilities to preserve zebrafish eggs in a fertilizable state. We collected coho ovarian fluid in Vancouver, B.C., Canada.
References below indicate how ovarian fluid is used.
Ovarian fluid is presently shipped by SeaTech on dry ice. As freeze dried samples are much cheaper to mail, we have freeze dried some coho ovarian fluid and will test it shortly for its efficiency in delaying fertilization of zebrafish eggs. We are also working towards developing a defined medium in collaboration with Derek Stemple in Wolfgang Driever's Lab. We have performed an extensive chemical analysis of coho ovarian fluid and hope by the summer of 96 to have a defined medium that has the ability to delay fertilization of zebrafish eggs . At that time we will make the formulation available to the zebrafish community.