Also, the widespread use of formalin fixatives makes many clinical samples inappropriate for the use of previously available TGF-Beta 1 antisera. Sections were dehydrated through graded alcohols and xylene and mounted with Permount. Formalin-fixed and unfixed neonate kidney samples showed positive staining in the lumen side of tubules and in vessel walls. In both adults and neonates, staining was seen exclusively on the cell surface with faint detection in the cytosol. While positive staining was detected with both formalin-treated and unfixed tissue, the use of a fixative yields better results.
Unfixed tissue has a typically distorted morphology compared to fixed sections.
Additionally, unfixed sections tend to dissolve during pronase treatment. Methods are described in the text. The image was captured at 200x magnification. Methods are described in the text. The image was captured at 200x magnification.