Towards a vaccine for HEV Earlier, we have shown the feasibility of using naked DNA constructs expressing pORF2 as a candidate HEV immunogen. As a protein immunogen, we have expressed a truncated mutant of ORF2 in insect cells as well as in Spodoptera litura larvae, using recombinant baculoviruses. Heterotypic interactions of HEV ORF2 and ORF3 proteins in yeast two-hybrid assays. Blue boxes show full-length and deletions of ORF2. Red boxes show full-length and deletions of ORF3.
White boxes show respective regions deleted in both genes.
His - depicts the growth of yeast clones streaked on synthetic dextrose plates lacking histidine.