Not most Biochemistry or Molecular Biology departments. Toward this end, our laboratory has focused on measuring intracellular metabolic processes not previously accessible to study in vivo. Our goal is to answer questions concerning their regulation by diet or other perturbations, and ultimately to apply the techniques in humans.
Much effort is spent on methods development.
For example, we have developed a new stable isotope method for measuring rates of endogenous synthesis of biomolecules such as fatty acids, cholesterol, glucose and proteins. And so on. Accordingly, our group is actually involved in a number of projects. Hellerstein M, Neese R, Linfoot P, Christiansen M, Turner S, Letscher A. Hepatic glucaneogenic fluxes and glycogen turnover during fasting in humans. A stable isotope study.