Jonathan Jones is creating a population of transposon mutagenised lines. For forward screens they are a poor tool, because there are too few insertions per plant. For GARNet a new population will be constructed with a high copy number of dSpm insertions. About 5,000 lines with about 30 independent inserts per line will be created. The lines will become available via the Nottingham Arabidopsis Stock Centre.
SINS can identify a potentially disrupted gene, and define the exact position of the insertion.
The FGT gene trap lines are being constructed under a different grant. SINS on the FGT lines will only be made available after a way has been found to bulk the seeds. Sean May and his staff at the Nottingham Arabidopsis Stock Centre will provide Bioinformatics for GARNet. GARNet intends to integrate with other functional genomics projects wherever possible. A European collaboration for GST primer exchange is also starting.