This method is based on AOAC procedure 954.02. Not directly applicable to canned, fresh, or frozen pet food. Dry such products at 70-110 degrees C., then grind and complete drying by AOAC 934.01. If the fat content falls below the guarantee proceed with this method. The fat content of a sample is determined by measuring the amount of material extracted from the sample by petroleum and ethyl ether after acid hydrolysis.
Sets usually consist of samples, one check sample and one blank.
Add 10 mls of alcohol and 25 mls of ethyl ether. Mix vigorously for 1 minute. Add 25 mls of petroleum ether and mix vigorously for 1 minute. Let stand until the liquid is practically clear. Decant, using transfer pipets, into 200 ml beaker containing a few glass beads.