Wash them in a solution containing CaCl 2 . 2. Stand 0 C for a while, heat 2 min 37 C, chill. Add growth medium, let the cells grow for 1 hr or so to express the antibiotic resistance gene. Spread a portion of the cells on a Petri dish containing the antibiotic. Incubate the plates for 24 hr.
Resistant cells grow, forming a bacterial colony.
The outcome of this experiment is that only those cells taking up the plasmid DNA should form a colony. Assume you have done the above experiment. You observe colonies on your plate. Give other hypotheses which are also compatible with your observation. All rights reserved.