Transfer the contents of tube A to tube B without taking out the tubes from the water bath. Add 20 m l of Superscript II RT Mix well. Maximal volume per column is 75 m l . The draining of the columns can be done outside the hot room. All the spins are done in microcentrifuges.
Place microarray filters individually into polypropylene tubes.
Take care not to crease them. Place on ice. Take into hot room. Place on ice. Add probe to hyb solution.