Incompletely coagulated samples may give false positive results due to fibrin deposition on the bead and subsequent trapping of the conjugated antibody. Reagents and equipments Abbott Auszyme Monoclonal Diagnostic kit. Single bead dispenser. 1 N sulfuric acid. Reaction trays Assay tubes with identifying cartons.
Procedure Handle all materials as though capable of transmitting viral hepatitis.
Mix by gently tapping the tray. Carefully add one bead to each well containing a specimen or control. Apply a cover seal. Gently tap tray to cover the beads and remove any trapped air bubbles. During the last 10 minutes of the incubation, prepare the OPD substrate solution.