This method was originally published by Ingrid Schmid, UCLA School of Medicine, Department of Medicine. It is a gentle fixation and permeabilization procedure for producing a suspension of fixed, permeabilized, and stained whole cells for flow cytometric assessment of relative DNA content, as well as surface and cytoplasmic antigen expression. It is suitable for cells derived from peripheral blood and suspension cultures.
Sodium azide is harmful if swallowed.
Keep out of reach of children. Keep away from food, drink, and animal feedingstuff. Wear suitable protective clothing. If swallowed, seek medical advice immediately and show this container or label. Contact with acids liberates very toxic gas.