Basic concepts to be discussed 1. Details of enzymatic reaction mechanism.
Structures are dynamic and change upon change of condition.
Use of low temperature and low pH to slow down reactions. Resolution of x-ray structures. 1 Porcine Pancreatic Elastase is a serine protease present in most animal species 2. Initial attack leads to an acyl-enzyme intermediate that is trapped in this case due to the relatively large size of Ile. Rcryst and Rfree are measures of the quality of the structure.