Infiltrate tissue in 2-3 changes of catalyzed resin 60 minutes each or overnight, depending on tissue and size. When fully infiltrated the tissue will become translucent. Using a cotton wool bud or swab smear accelerator onto the base of each mold, then add polymerized resin and finally the tissue, allowing it to sink to the base rather than applying pressure. 20 minutes Polymerization is an exothermic reaction and it is important to cool the molds in a bath of cold water to disperse the heat produced. Sections can be obtained from 1-5u, floated onto a warm water bath picked up onto clean slides and dried on a hot plate at 60 degrees C for at least 30 minutes.
Most routine stains give good results on tissue.
Most routine stains give good results on tissue embedded in Histocryl using standard times and temperatures, although it may occasionally be necessary to extend some staining times.