By using these standard microbeads, you can perform quantitative quality control and obtain quantitative immunophenotypic measurements. The kit has a series of 5 beads with well-defined quantities of a primary CD5 MAb in ABC units ranging from 2,500 to 500,000. The beads mimic cells which have been labelled with a specific MAb.
Titration is needed to ensure saturation.
A calibration curve is constructed by plotting the fluorescence of the beads against the ABC units on the beads. The kit provides Blank beads and Calibration beads. The calibration beads have different fluorescent intensities that can be excited by any wavelength of light from 365 to 650 nm. The program is, in conjunction with fluorochrome or antibody-coated beads, designed to calibrate the fluorescence channels and to monitor the performance of your flow cytometer. The program is also useful in conjunction with confocal microscopy.