Also one generally thinks of ovules being fertilized, not ovaries. The final section dealing with Polyploidisation concentrates almost exclusively with treating plantlets, seeds or roots with colchicine. Only one technique uses nitrogen protoxide and it is sketchy, at best. Techniques are given to determine if polyploidisation has taken place. These include measuring of guard cell length, counting of chloroplast number per guard cell, determining the number of nucleolar chromocentromeres, counting the number of stomata per given area and measuring diameter of pollen grains.
Nothing is said but, one assumes the bigger or more abundant indicate an increase in ploidy level.
The Annexes, or Appendices, contain very little information. The recipes for three stains are presented along with instructions for making permanent mounts and taking photographs. Many of the individual protocols had their own instructions for preparing stains, culture media and fixatives so were not included in the Annexes. However, many of the recipes for stains and other chemical solutions were not included and it will be necessary to find them in other sources. As presented in the Introduction, this book is intended for use by teachers and students and as an indispensable tool for researchers and laboratory technicians.