Rehydrate the conjugate with 1.0 mL of IFA buffer. Remove the required number of slides from their foil pouches. Positive, Negative, Buffer Controls must be run each time the test if performed. Allow the slides to reach room temperature. Place slides in a covered, moist chamber and incubate at 37 C 2 C for 30 minutes.
Rinse slides briefly in a light stream of buffer.
Rinse in a staining dish of buffer for 15 minutes. Change the buffer twice during this time. Buffer should be agitated in a magnetic stirrer or by moving the slide rack up and down. Blot the painted mask of the slide with the blotters provided. Do not allow the wells to dry before conjugate addition.