Place slides on 37 C warmer 1-2 minutes before adding probes. Denature probe mix at 70-75 C for 5 mins. Apply denatured probe immediatly onto warmed slide on the hot plate. Let rubber cement dry 5 mins on warmer. Incubate for 2-3 days at 37 C in a humid chamber.
Slide coverslips off gently.
Rinse slides 2X in ddH20 for 5 mins each. Cot DNA varies from lot to lot. We have requested that a single Lot be put on reserve for our use. The amount of probe DNA used can vary, and should be adjusted depending on the intensity of the product. 45 ul Probe size Probe size is probably the most important aspect of CGH success.