1. 3' end of growing backbone 2. Synthesis in 1 bubble then moves toward synthesis in adjacent bubble. Synthesis then progresses around the circle. Must start a new primer using primase Then DNA polymerase III can act and add to 3' end of 2nd RNA primer on lagging strand.
Molecule continues to open up and leading strand synthesis continues continuously.
Cannot join ribose to deoxyribose. Leaves a nick between end of new DNA and DNA segment of adjacent Okazaki fragment. NO base, sugar, or phosphate is lacking. Ligase seals this nick. Ligase neither adds nor removes nucleotides.