RAPD products are routinely resolved on agarose gels and visualized by ethidium bromide. This provides a rapid method of scanning a genome. RAPD is widely used because of its simplicity and low-cost instrumentation. Of all MAAP procedures, DAF utilizes the shortest primers, down to 5 nt in length. DAF products are routinely separated by thin polyacrylamide gels, backed onto plastic Gel-Bond film.
Resultant gels are air-dried and kept for permanent record and evaluation.
DAF products were also resolved by alternative methods. The resultant amplification products were separated on an ABI Sequencer using Gene Scanner software. Single nucleotide resolution was obtained for lower sized amplification products. In general, DAF generates scoreable polymorphisms in the molecular size range from 100 to 800 bp. The ability to detect molecular markers closely associated with genes of agricultural importance makes marker-based breeding an attractive proposition.