An appropriate paradigm for development of technology and approach to genomes of larger eukaryotes e.g. man. Genome-wide expression data obtained using oligonucleotide chips or high density microarrays.
Quantification is a concern.
The commercial ORF expression library of all yeast genes expressed in both yeast and mammalian cells. Tagged for identification and protein isolations. Study your protein product in vitro or see what it does in living cells when overexpressed. Protein-protein interactions addressed through a massive two hybrid search. Creation of appropriate ORF libraries for all 6000 genes versus current random cDNA or genomic libraries.