The J5 Model gradient former is designed for pouring polyacrylamide gradient minigels or for pouring sucrose gradients into small centrifuge tubes up to 5 ml in total volume. The dual-piston design is intended to keep the fluid level in each chamber equal during gradient formation, regardless of the differences in density or viscosity of the two fluids being mixed. A trapped air bubble will not stop the flow, unlike gravity flow types, the company asserts. When the gradient former is attached to a peristaltic pump, the pistons are automatically drawn downward to keep the levels equal.
Researchers also can dispense liquids manually by turning the lead screw to advance the pistons.
It consists of a VialServer, CE module, and UV3000 scanning detector, all of which are controlled by the company's PC1000 software. With up to 110 vial positions, the VialServer offers sample thermostating, automatic buffer mixing, buffer replenishment, and pre-capillary sample derivation. The system accommodates any combination of chemistry, voltage, current, pressure, and temperature for any type of application. It will apply voltage or current at either end of the capillary. Occludin is an integral membrane protein involved in the establishment and maintenance of tight junctions.