Add 4.0 mL of the extraction solvent to each vial. Cap the vials and shake them on a mechanical shaker for 1 h. 3.5. Measure detector response using a suitable method such as electronic integration. Construct a calibration curve using an external standard method by plotting g per sample versus detector response of standard injections. Synthesis of MAVA 4.12.1.
Procedure Recrystallize the maleic anhydride from toluene.
Stir the mixture for 10 min. Evaporate the chloroform in a hood. Dissolve the residue in a minimal amount of chloroform. While heating on a hot plate, slowly add isooctane until the solution just becomes cloudy. Clear the solution with an addition of a drop of chloroform.