Transduction - transfer of DNA from one bacterium to another by phage. I. Classic experiments with phage A. One step - Dilute or inactivate unattached phage to insure synchrony More phage upon lysis with chloroform suggests progeny collect inside host. Complementation groups Recombination frequencies to study precise gene location. Cytosine modified to 5-hydroxymethylcytosine, methylation by host RE system.
Many proteins required since structure is complex.
Some use proteases to produce precursors and coordinated assembly. P22 uses scaffolding protein to build virion, but is not part of final product. E. Lysis and release - T4 lysozyme and lipase. No ds replicative intermediate - breaks apart before completion of replication, so not degraded by host enzymes that destroy dsRNA. 1. Infecting RNA is polycistronic with 3 RBS - No regulatory proteins produced.