They are located inside the foil pouch. All wells in the extra strip are NSB's. The preferred sample is unstimulated whole saliva collected by passive drool. The assay of salivary testosterone is not appropriate in samples collected using cotton swabs, cotton or polyester versions of the Salivette device, or the Salisaver device. Freeze all saliva samples prior to assay in order to precipitate mucins.
Procedure Bring all reagents to room temperature except concentrated enzyme conjugate.
Keep this at 2-8 C until needed. Label five microcentrifuge tubes or other small tubes 2 through 6. Pipette 150 l of assay diluent in tubes 2 through 6. After changing pipette tips, remove 100 l from tube 2 to tube 3. Pipette 18 ml of assay diluent into the disposable tube.