However, indirect methods can result in the formation of complexes that will artifactually stain Fc receptor binding cells. The biotin-avidin method gives the least amount of artifactual staining particularly when streptavidin conjugates are used. However, indirect methods can be used to determine the relative brightness differences between cell populations. Refer to the Ficoll-Paque package insert for materials and reagents required. Store at 2 to 8 C. Do not use PBS containing biotin with the biotin-avidin indirect method.
Sodium azide is harmful if swallowed.
Keep out of reach of children. Keep away from food, drink, and animal feedingstuff. Wear suitable protective clothing. If swallowed, seek medical advice immediately and show the container or label. Contact with acids liberates very toxic gas.