Delta T takes care of the fluid, thermal, and optical environment therefore all you have to do is place the specimen on the adapter. Perfusion ports are included to enable separate perfusion of both sides of the specimen. The Specimen is placed on the aperture and supported by a Silastic band. This defines a plane for the specimen. The specimen is then lowered into the Delta T dish where the height of the specimen can be adjusted relative to the focusing distance of the objective.
Special configurations are available for a variety of specimen consistencies.
The Bioptechs Brain Slice adapter is designed for the observation of temperature controlled specimens that have to be maintained in a perfusable micro-observation environment on an inverted microscope. They are 5mm high and available in a variety of inside diameters including, 4mm, 6mm, 8mm, 10mm, 12mm, and 14mm. The outer diameter is always two mm greater than the inside diameter due to the 1mm wall thickness. The cylinders are made of Pyrex glass and are heavier than plastic cloning rings to eliminate floating. They are optically polished on the bottom surface to mate with and form a tight seal with other glass surfaces such as coverslips and Delta T dishes.