These solutions should always be kept cold. Solution A ready before proceeding. Plus Solution B. Stir well and place into an ice bath while embedding to retard premature polymerization. Anaerobic conditions are needed for polymerization. Polymerization is complete at room temperature in 1-2 hours.
Polymerization proceeds more rapidly in larger batches.
Remove the specimen cast from the mold and carefully wipe off any unpolymerized resin solution. Optimal sectioning is performed with a microtome designed for plastic embedments. 0.5 micron-3 micron sections are cut with a dry glass or diamond knife, collected with forceps, and transferred onto a room temperature water bath surface, releasing sections before they touch the water. Dry sections are stained directly without xylene or alcohol pre-treatment. Longer staining times or higher stain concentrations may be necessary for thin sections.