Mengen isolieren lassen. Virusproduktion und langwierige Titerbestimmungen erfordert, soll die Kapsidherstellung ber integrative Plasmide in Drosophilazellen etabliert werden. The formyl group of FGly is directly involved in sulfate ester cleavage. In eukaryotes the modification is catalyzed by lumenal components of the endoplasmic reticulum posttranslationally and prior to folding of sulfatases.
Modification is directed by a short linear sequence motif surrounding the cysteine.
Sulfatases are the only known targets of this posttranslational modification. So far the enzymatic machinery involved in modification of cysteine into FGly remains unknown. We try to characterize it biochemically and to purify it from a crude extract of lumenal components of the ER using a number of chromatographic techniques with the following identification of proteins. GC-Resistenz entwickelt werden. Andrea Heyer Frau Prof.