The bulk fluid was at the top and the substratum upon which the biofilm grew was at the bottom of the image. The fluorescent color after acridine orange staining was shown to reflect the relative growth rate of the microorganisms.
Orange indicates rapid growth and green indicates slow or no growth.
Rapid growth occurs in the biofilm near the biofillm-bulk fluid interface. In places where the biofilm is thin, microorganisms throughout the biofilm are growing rapidly. Scale bar is 100 microns. Home Site Map Resource Library Industry Connection Workplace Contact Webmaster Terms of Use Links marked by the 3-arrow sign take you to sites with content for which the CBE is not responsible.