A NIH funded postdoctoral position is available immediately to study the folding of several proteins in the intracellular lipid binding protein family. This project examines the folding mechanism of proteins with very similar structures but different sequences, emphasizing a structural genomics approach to the folding problem. Experience in recombinant DNA techniques and steroid biochemistry preferred. Molecular genetic and biochemical approaches are being used to determine how the CAD and KID interact with each other and with other regulators to facilitate the assembly of a transcription initiation complex, its isomerization to an active state, and release of the polymerase to transcribe the target gene. Isom Postdoctoral positions are available immediately to study cellular and molecular aspects of liver pathophysiology in hepatic cell systems.
Position requires experience in molecular biology.
Tax-induced gene expression during the course of adult T cell leukemia and neurodegenerative disease. This project examines the role of cytosolic calcium and annexins in the differentiation process of chondrocytes. Using site-directed mutagenesis, fluorimetry and transfection experiments, this project will investigate the mechanisms of annexin channel formation and its role in terminal differentiation and mineralization of chondrocytes. Candidates who wish to work with our mutants must have a strong genetics background. Model systems include human colon and breast cancer cells and untransformed intestinal and mammary epithelial cells.