Cell Culture Materials Protocol Method Microscopy Troubleshooting Microtubules are involved in many aspects of cell motion including propulsion, mitosis, growth, and organelle transport. Microtubules are easily stained using an anti-tubulin primary antibody and a corresponding secondary antibody conjugated to any fluorochrome. Their regular size and fine structure provide beautiful images. The following procedure reveals smooth and linear microtubules and can be used with a wide variety of other antigens as well.
Most cell types contain microtubules.
Spreading, adherent cells are easier to stain than suspended cells and provide the best microtubule arrays. Grow the cells of interest on 1.2 cm round coverslips in 24-well culture dishes. Cells should be well-established but subconfluent to allow full development of the microtubules. 900ml Bring to pH8 with NaOH before making up to total volume. 25 mg NaBH4 into each of three 50 ml plastic culture tubes.