Only two-point linkage tests were possible, so the orientation on the chromosome with respect to the marker could not be determined. Tests with aneuploid stocks deficient for the short arm of chromosome 12 placed the gene in the long arm of chromosome 12. Transgenes can be inserted as single events, are stable, and segregate with Mendelian expectations. The typical procedure in improved cultivar development is to select and utilize single-event gene insertions. Knowledge of the location of the inserted gene would provide the opportunity to develop germplasm and strategies to maximize the efficiency of developing improved germplasm.
Transformation is considered to be a random event, with each inserted gene at a unique location.
So, each transgenic germline is the result of a separate insertion event, and the location of the insertion in the genome could affect future success in attempts at backcross improvement, depending on associated linkages.