The state of the art may have changed since the publication date.
Many enzyme assays are tedious, requiring the addition of reagents at multiple time intervals.
The HPLC-based analysis of reaction products requires an additional step of vialing the samples and placing them in the autosampler. Such time-consuming, repetitive procedures are ideally suited for automation. We automated an HIV protease assay for the purposes of screening compounds as inhibitors of HIV protease and determining inhibition constants. The resulting automated method produced inhibition constants that were of comparable accuracy and precision to those determined by manual methods. National Library of Medicine.