After micro-centrifuging off the unbound genomic DNA and protein, the bound plasmid DNA is simply eluted from the filter and is immediately ready for use in sequencing or transformations. The purified DNA can also be ligated, when required, by restriction enzymes.
Results are good for both high and low copy plasmid.
A complete protocol is supplied with each kit. It is advisable to wear gloves and eye protection throughout the whole procedure. With care, remove and discard the supernatant. Centrifuge at approximately 12000 x g for 5 minutes. Cap the tube and centrifuge for 1 minute.