As anthers will not dehisce in a damp atmosphere, it is essential to keep the humidity low in these pots. If this is done aseptically, it obviates the need to sterilise the seeds for the next generation. To test for transformation, F1 seedlings are germinated on GM K50. Put seeds on GM K50. Seal with Micropore tape.
Sensitive seedlings form neither roots nor leaves, and have white cotyledons.
Transformed seedlings are phenotypically normal.