By knowing the amount of internal standard added to the reaction, one can determine the amount of target DNA present, in this case Mycoplasma. This quantitative competitive method was designed in our laboratory to quantitate M. incognitus levels over a large linear range, which controls the differences in amplification variability seen with diverse clinical specimens. Quantitation of Mycoplasma DNA levels in clinical specimens is particularly helpful in the evaluation of antibiotic therapy as well as patient stratification. Send by overnight delivery. Culture of Mycoplasma species is expensive, time consuming, labor intensive and it can take up to 6 weeks before becoming positive.
Serological methods are easier to perform and less costly.
However, they are also generally non-specific, insensitive, and retrospective. PCR-based technology for Mycoplasma yields the highest level of sensitivity and specificity. For information regarding these and additional tests, feel free to call us .