Quenching procedures for autofluorescence 1. Eriochrome black . Sodium nitrite 4. 37 o C for 20-30 minutes 8. Mayer s hematoxylin counterstain 9.
Nitrocellulose paper is cut into narrow strips.
Use forceps to handle paper. Wash the paper 5 minutes by gentle agitation in distilled water. Let dry at room temperature. A diluted solution or suspension of antigen in saline is dotted onto the filter. Less complex antigens can be diluted accordingly.