If you view the entire column as the membrane, then only the glucose should pass through, starch should be left behind- which obviously is not the case. If you view each gel bead as the membrane, there is no difference. Sodium chloride elutes the solutes through the gel column as well as cleansing the gel column at the conclusion of the lab. Sodium chloride also keeps the concentration of the salt the same both inside and outside the beads. Ten drops are collected first, as the bottom of the column contains only salt.
Salt comes through the gel column with the starch.
This could be proven by adding lead nitrate to any fraction collected, and obtaining a white precipitate. Purification of biological macromolecules e.g. proteins, nucleic acids, photosynthetic pigments, inorganic mixtures. The gel, LH-20, is required. To separate starch from glucose in a mixture. State a hypothesis concerning the outcome of this experiment based on background information.