Qualifications of individuals performing surgery. Survival surgery is not done in the aseptic surgery suite. It is done in an area set aside for surgery that is easily sanitized. The procedures are done using sterile instruments, surgical gloves, and aseptic procedures. Postoperative care and records are the responsibility of the Principal Investigator and his staff.
Postoperative care is outlined in the protocol.
Euthanasia a. Methods for Each Species - Mice are euthanized by carbon dioxide, and cervical dislocation with justification. Rats by carbon dioxide, decapitation after sedation, and sodium pentobarbital. Opossums by carbon dioxide or sodium pentobarbital. Dogs by sodium pentobarbital. Turtles by decapitation after hypothermia and frogs by double pithing after hypothermia.