They have symptoms of vomiting, nausea, and watery diarrhea, suggesting a possible coliform contamination. What would you expect to see on the plate or broth and explain how the medium selects for coliforms. Non-lactose fermenters are colorless c. Now that you suspect a coliform is present, you attempt to enumerate the amount of bacteria in the drinking water by using MPN in BGLB broth. You use a standard three tube, five dilution method.
Results are below.
All positive tubes have growth and gas. Yes it would invalidate the test. You eventually obtain two bacteria from the water sample and run a battery of tests to find out who they are. Below are listed the tests. You want to isolate a Streptomyces able to degrade starch from a sample of water.