The quality of graphics in this document is inferior to the printed form and may vary considerably, depending on your browser, monitor type and individual printing capabilities. If you need a printed copy of the document, please contact us. Promega Corporation Promega's new Altered Sites II Mammalian Mutagenesis System combines site-directed mutagenesis with mammalian expression capabilities. The system allows for efficient mutagenesis and easy expression of the mutagenized sequences without additional subcloning steps. Introduction Expression of cloned genes in mammalian cells is a useful technique to study cellular processes, protein modification and protein function.
Some vectors also contain an intron which increases expression levels of many cDNAs.
Often, expression studies require mutagenesis of a specific gene. Achieving the desired mutation may involve a number of subcloning steps that can significantly increase the time from the initial expression studies to transfection of the newly mutant sequences. The Altered Sites II Mammalian Mutagenesis System is designed for efficient, easy mutagenesis with high yield. The mismatch in the oligonucleotide is designed to produce the desired mutation. Following hybridization, high fidelity T4 DNA Polymerase extends the oligonucleotide to create a duplex structure.