A footprint indicated by the arrow coincides with the area irradiated by the electron beam. Bar is 1 micron. Bar is 1 micron. 5.Computer processed EDP obtained from glucose-embedded catalase crystal on holey carbon coated with 30 of carbon on both sides by thermal evaporation. The crystal was tilted to 45 o .
Tilt axis is indicated by lines.
The pattern was processed to remove the radial background due to inelastic scattering and the spike. The crystal was tilted to 45 o . The lattice directions are indicated along with the tilt axis. Reduction of charging in protein electron cryomicroscopy. J. Microscopy, in press.