Therefore, although Vm does not change, more product is produced. If we double the amount of enzyme, in a substrated saturated solution, we double the amount of product produced. Enzyme Affinity Affinity has to do with how well a substrate binds to the enzyme. A substrate with an increased affinity will turnover quicker and thus increse the amount of product produced, Vm is increased. As mentioned previously, modulation impacts the enzyme activity, and, thus, the reaction rate.
Modulation is used to control the rate of reaction. .
A sequences of reactions called metabolic pathways. Each reaction in the pathway has its own reaction rate. This rate is controlled by the factors previously mentioned. Finally, an interesting development with metabolic pathways, is that it is possible for a product of a subsequent step to modulate a previous step. That is, it is possible for a reaction product to inhibit the whole pathway and thus limit the rate of product production.