To remove sediment, RDO may be filtered, if desired, without altering its effectiveness. To achieve optimal performance, do not reuse RDO . Since the nature of a decalcifying agent is to release calcium ions from the bone into the acid solution, as the solution becomes saturated with calcium ions, the decalcification process will slow down. Addition of an alcoholic solution can aid in preventing undue swelling and hydrolysis of the tissue. It will, however, slow down the decalcifying agent.
Rinse specimens thoroughly in running tap water after decalcification.
USE RDO is a very rapid bone decalcifier. The acid component of RDO reacts with calcium in mineralized tissues to form soluble calcium salts. The little extra care required in its use will result in routine sections with superb histological detail and staining characteristics in a fraction on the time required with other decalcifiers. Tissues should be thoroughly fixed before decalcification. Most standard fixatives can be used prior to RDO use.