The procedure yields similar results with small and large amounts of blood. The most important pitfall of their method was that they advocate the use of defribrinated blood. This could lead to serious cell loss and activation. As they had observed, monocytes bind strongly to small microscopic blood clots. The adequate blood anticoagulation is then critical.
Temperature is also a critical point.
MCA was supported by a Capes fellowship. MBN and AB are Senior Investigators of CNPq.