Triethylamine was shown to be an effective additive for optimizing resolution of amino acids and biogenic amines. Analytical Biochemistry 231, 440-446. Determination of 2-Oxohistidine by Amino Acid Analysis. Identification of phosphocysteine by electrospray mass spectrometry combined with Edman degradation. During Edman sequencing, phosphocysteine is degraded primarily to cysteine and phosphate.
Sensitivity is in the low pmol range.
Analytical Chemistry 67, 4170-4177. Analytical Chemistry 67, 4197-4204. This procedure minimized sample handling, where the majority of losses occur. Improvements in the Characterization of Glycopeptides and Glycoproteins Using Liquid-Phase Separations and Mass Spectrometry. Current Opinions in Structural Biology 5, 691-698.