Wipe backs of slides and air dry upright on kimwipes. Place slides on 37 C warmer 1-2 minutes before adding probes. Apply denatured probe immediatly onto warmed slide on the hot plate. Let rubber cement dry 5 mins on warmer. Incubate for 2-3 days at 37 C in a humid chamber.
Slide coverslips off gently.
Incubate for 5 mins, then gently remove coverslip . Gently cover with a 24 X 50 mm coverslip. Gently remove coverslip. Rinse slides 2X in ddH20 for 5-10 mins each, air dry upright. Let sit at RT for at least 2 hours.