Signed and witnessed information consent form. Negative medical history of heart disease, kidney disease, cancer, bleeding ulcers, diabetes, jaundice, liver disease, hepatitis and epilepsy. Negative medical history for bacterial or viral infections that may be hazardous to researchers. Neupogen is administered daily for four consecutive days, followed by apheresis on day five. Peripheral blood mononuclear cells are collected by a single apheresis using a Cobe Spectra.
Vascular access is through arm veins only.
The amount of whole blood processed is 10-12 liters. In addition, Blood Chemistries are performed one month following apheresis, and Complete Blood Counts are done at one month, six months, and annually for five years.