Gene 1 Gene 2 Gene 3 Protein 1 Protein 2 Protein 3 DNA contains specific regions called genes which codes for a specific protein. A gene is not a continuous stretch of genetic code. Exons mRNA Gene Splicing Gene A gene contains exons , which code for the protein, interspersed by introns , which constitute non-coding regions. This process is known as gene splicing . This mRNA in turn is transcribed to produce a protein.
Prokaryotes lack introns, whereas eukaryotes contain introns.
Now our objective is to locate a particular gene in the nucleotide sequence. Then locate exons and introns with in the gene. As a first step in any gene identification analysis, locate and remove interspersed and simple repeats from eukaryotic sequences. These repeats rarely overlap promoters or other coding regions. Thus their locations can provide important negative information on the location of gene features.