Allows for the uptake of large plasmids. DNA specific endonuclease I. Mutation shown to improve yield and quality of DNA from plasmid minipreps. F' F' episome, male E. coli host. Necessary for M13 infection. Confers resistance to nalidixic acid.
Mutation increases lambda lysogeny by inactivating specific protease.
D lon Deletion of the lon protease. Reduces degradation of b-galactosidase fusion proteins to enhance antibody screening of l libraries. Inability to utilize maltose. Gene central to general recombination and DNA repair. Mutation eliminates general recombination and renders bacteria sensitive to UV light.