The untreated or enzyme-treated serum was diluted as outlined below and used in immunoprecipitation experiments to characterize the antibodies. The results of the reactions in which the untreated anti bodies were used to immunoprecipitate DNP-BSA are presented below. Predict the results that should be obtained at the different dilutions for each set of circumstances outlined below. Give a brief explanation for each set of results. Illustrate and label the structural components of the secreted form of IgG1, the membrane form of IgM and the secretory form of IgA. Be sure to include all of the accessory molecules, disulfide linkages, points of enzyme attack and domains th at mediate the major biological functions of these immunoglobulin isotypes.
Positive activation leads to responsiveness, negative activation leads to tolerance.
Briefly outline the intrinsic and extrinsic properties which convey immunogenicity and antigenicity to a molecule. Complement component C3 is a 180,000 dalton protein that plays a key role in complement activation and the cleavage products of C3 are important mediators in the some of the biological activities that result from complement activation. Discuss the role of C3 in the activation of the classical and alternative compleme nt pathways. It is decided that immunostaining of histological sections of lymph nodes and spleen are the best way to study the responses. You are given access to a large library of antibodies directed against different cell surface alloantigens.