The cell membrane remained in contact with the microtubules. Pulvini were also subjected to a ABA solution to promote shrinkage of cells and both high and low light conditions were tested. Preliminary results indicate that colchicine prevents microtubules from depolymerizing when samples are transferred from low light to high light and thus the cells cannot shrink as much as control cells. Results on taxol are inconclusive at this point due to the fact that neither taxol treated nor control cells exhibit sufficient reswelling when transferred from high light to low light. Microfilaments appear to aggregate near the cell membrane in cells subjected to high light.
Results are inconclusive due to poor visualization.
In conclusion microtubule conformation is changed drastically when motor cells undergo rapid size change. Inhibitors prevent microtubules from polymerizing and depolymerizing in a normal way which may influence the overall function of the pulvinus during light avoiding movements.