Sample Preparation Centrifuge Gradients Sucrose Solutions Cesium Chloride Gradients Gradients used in ultracentrifugation are difficult to count.
More concentrated solutions require a special procedure.
Dilution of concentrated CsCl gradients is essential to achieve homogeneous counting conditions without self-absorption quench. With minimal effort, a special CsCl solubilizing solution can be prepared. Shake well and count. Shake and count. For ambient temperature counting only.