Optical sectioning microscopy and deconvolution greatly improve image resolution. Optical sectioning also makes 3-D rendering possible. The YRC acquires and deconvolves images using the DeltaVision microscope and software system. We present an example of the power of the deconvolution process to visualize the yeast mitochondria. If you have GFP fusions already made, we can quickly produce the initial three-dimensional images of its localization.
More extensive collaborations are also possible.
We present a brief introduction to FRET and an example of FRET between CFP and YFP which are both linked to the histone Hhf2p. The fluorescence microscopy group joined the YRC in 1998 with members Dr. Trisha Davis, Dr. Eric Muller, Dale Hailey and Bryan Sundin.