See Below for more details. Annotated Instructions available . You may either hand enter the number of mismatched bases or let the computer count them by changing their alphabetical case and choosing the approriate mode on the selector. Simply insert as many underscores as neccessary in place of bases. This is handy when cutting and pasting data which includes numbers, spaces and punctuation.
Most Browsers allow results to be cut and pasted in to other applications.
Alas, some do not. Our oligomers are typically 25 to 45 bases long and contain 1 to 3 base mismatching mutations. We try and keep the mutations no closer than ten bases of the ends of the primer. We use these primers in PCR to create a plasmid containing the altered gene. The above calculator renders finding approriate primers that bracket the desired mutation a trivial matter.