The sera from the two rabbits is used to probe the membrane to determine which peptide of the ninety-six gave the strongest response. The antigenic peptide, so designated by the rabbit's own immune response, is resynthesized as a linear peptide, conjugated to KLH, and then reinjected into the same animals. Finally, the sera is affinity purified by the linear peptide. The result is that the researcher receives antisera raised against the native but purified against the peptide. The problems found by using whole protein for antibody production may be eliminated by careful selection of the peptides bound to the membrane.
Price is for basic service.
Additional fees will apply for special requests. Careful selection of the screening is necessary to achieve desired results. Synthesize two 48 peptide epitope maps. We will select the peptide based on regions which are found only on your protein of choice.