All prices and specifications are subject to change without notice. This document is designed primarily for online viewing. The quality of graphics in this document is inferior to the printed form and may vary considerably, depending on your browser, monitor type and individual printing capabilities. The introduction of foreign DNA into eukaryotic cells has greatly contributed to our understanding of many biological processes. Numerous transfection systems have been developed and optimized in a variety of mammalian cell lines with the goal of reaching high levels of foreign protein expression.
Vector is included in transient transfections.
As with many cellular control mechanisms, the initiation of translation is controlled through reversible phosphorylation and dephosphorylation of protein factors. Figure 1A summarizes normal eIF-2 involvement in initiation of translation. This complex then binds to the 40S ribosomal subunit, which initiates a sequence of reactions leading to the assembly of the 80S initiation complex. During the 80S complex formation, the GTP is hydrolyzed to GDP and is finally released in a complex with eIF-2. DAI is one of several enzymes involved in the host cell's antiviral defense system.