Molecular genetic and biochemical characterization of transcription and nucleosome restructuring at target promoters in these controllable mutants will provide further insight into Snf-Swi function. In contrast to Snf-Swi, RSC is essential and abundant. We have shown that Sth1p possesses a DNA-stimulated ATPase activity critical for function, and that Sfh1p is a phosphoprotein whose phosphorylation is cell cycle regulated.
Recent studies implicate involvement of a mitotic checkpoint pathway.
Genes Dev . Cell Biol . Sfh1p, a component of a novel chromatin-remodeling complex, is required for cell cycle progression. Favorite links from the BC Laurent lab. Our laboratory is supported by a grant from the National Institutes of Health.