This particular phenomenon has become the basis for a newer technique of measuring distances far below the lateral resolution of the light microscope. The occurrence of bleaching has led to a technique known as FRAP, or Fluorescence Recovery After Photobleaching . FRAP is based upon bleaching by short laser bursts and subsequent observation of the recovery of fluorescence caused by the diffusion of fluorophores into the bleached area. Also effective for Rhodamine. Reagent blackens when subjected to light exposure so it should be stored in a dark place.
Skin contact is extremely dangerous.
Although its effect is slightly lower than p-phenylenediamine, it is more resistant to light and features a higher level of safety. G. D. Johnson et. To lessen fading in some specimens, it may be advisable to use a neutral density filter in the light path before the light reaches the excitation filter, thus diminishing the excitation light intensity. For photomicrography or visual observation, rapidly changing the field of view may also avoid fading effects. Authors Mortimer Abramowitz - Olympus America, Inc., Two Corporate Center Drive., Melville, New York, 11747.