Saturation binding experiments measure equilibrium binding of various concentrations of the radioligand. Analyze the relationship between binding and ligand concentration to determine the number of sites, Bmax, and the ligand affinity, Kd. See Introduction to saturation binding experiments Competitive binding experiments measure equilibrium binding of a single concentration of radioligand at various concentrations of an unlabeled competitor. Analyze these data to learn the affinity of the receptor for the competitor. Kinetics experiments measure binding at various times to determine the rate constants for radioligand association and dissociation.
Prism makes it easy to analyze and display data from all three kinds of experiments.
A short cours e on theory and methods , Martinus Nijhoff Publishers, second edition, 1996.