Carefully open bags or uncover containers before starting autoclave. The load must not exceed the capacity of the unit. Steam saturation is essential for maximum heat transfer. Air pockets or inadequate steam supply will cause sterilization failure. Water can be added to the containers to add moisture.
Most people are aware that autoclaves contain live, pressurized steam.
Burns can result if the autoclave door is not properly seated or if the autoclave is opened while there is still pressure in the chamber. If you are not sure how to prepare the autoclave, seek help before starting the run. Infectious or biohazardous waste must be transported from the laboratory to the autoclave in lidded, leak proof containers. Containers must be labeled to indicate the laboratory of origin, the agents present, and the fact that the material has not been autoclaved. Wear heat-resistant gloves and a lab coat when removing items from the autoclave.