A high level of gene expression was detected in melanoma tissues and melanoma cell lines. Treatment of melanoma cells with H11 specific antisense oligonucleotides, inhibited H11 expression and resulted in a marked decrease in cell proliferation. Prognostic tool to determine whether, after tumor removal, surgical margins are free of cancer. High antigenicity makes it likely that serological assays can be developed. Antisense chemotherapy targeting H11 to inhibit tumor growth.
Protein is highly antigenic.
Antibodies are available. They stain formalin-fixed tissues and work in immunoprecipitation, western blot, and immunofluorescence. Demonstrated that antisense oligonucleotide inhibits melanoma cell growth. Animal efficacy studies for gene and immunotherapy. Aurelian, L., Smith, C.C., Winchurch, R., Kulka, M., Gyotoku, T., Zaccaro, L., Chrest, F.J., Burnett, J.W. J. Invest.