A gene library is a collection of recombinant plasmids that have inserts comprising most or all of the DNA sequences of a given organism. Together, the millions of recombinant plasmids would encompass the entire human genome. PCR depends on a special enzyme called Taq polymerase, which can make copies of DNA, even after being heated to temperatures near boiling. PCR also depends upon short DNA primers, which must be the same as the sequence at either end of the target gene. PCR reaction mixture includes Template DNA Short DNA primers to act as anchors Taq polymerase.
Primers bind at both ends of target sequence.
Temperature is lowered and Taq creates new daughter strands. Cycle is repeated dozens of times, with the number of identical daughter DNA molecules doubling each time. The University of Minnesota is an equal opportunity educator and employer.