The Theoretical Biophysics Group at the Beckman Institute at the University of Illinois has launched the Structural Biology Software Database , a database of programs of utility in the fields of structural biology and bioinformatics. The Genome Channel maintained by Oak Ridge National Laboratory provides direct access to sequence data from a wide range of organisms. Application notes on nucleic acid chemistry are available at the Bioresearch section of the Beckman corporate Web site. Genosys maintains a library of technical notes on the characterization, analysis, and purification of oligonucleotides. Surf the Midland site if you need to calculate the molecular weight of an oligonucleotide.
Midland specializes in mass spectrometry of oligonucleotides.
The Genosys site provides short primers on UV quantification of DNA, purification methods, and melting temperature. The Operon site provides an oligonucleotide toolkit. If you provide the sequence, the toolkit will calculate useful properties such as the molecular weight and the melting temperature. Your Web browser must be Java-enabled to use the toolkit. PanVera Corporation provides an on-line tutorial on the PCR technique at its Web site.