Measure changes in microbial abundances and inorganic and organic N concentrations over time. Adjust salinity of source water to match that of receiving waters using precombusted salts, if necessary. Control water should be deionized water, adjusted to the proper salinity, if necessary.
Prepare bacteria concentrate from receiving waters.
Cap the flasks with foil and incubate in the dark at 20 degrees C with gentle stirring. Concentrations of organic N decreased over time while an almost equal increase in DIN concentrations was observed. We are always interested in expanding our knowledge on the bioavailability of DON from different sources.