However, the approach developed here may be much more general to other templates . The cells used in the study cited above have low affinity binding sites for both tPA and plasminogen. These sites have not been fully characterised, but, since tPA and plasminogen can compete for binding, the number of sites for each reactant is set to the same number per cell. Some of these were determined, others were taken from the literature. These are shown in the table below.
Parameters can be varied to investigate the regulation of cell-associated enzyme activity.
Find out here where this expression comes form. It can be thought of as the concentration factor for the reaction. Another way of looking at it is the ratio of observed concentration of reactants found around the cell, relative to the concentration of reactants added to the whole reaction mixture. R i is also affected by the cell concentration. Lambda can be viewed as the concentration factor for a single cell and will be dependent on the height of the shell around the cell where the reaction takes place.