Myf5 have redundant functions. Name 2 cellular components that are required for axis determination in Fucus embryos. Shh mutants exhibit some Pax-1 expression, indicative of limited sclerotomal development. When high concentrations of Noggin are applied to presomitic mesoderm explants, it causes the induction of Pax-1 expression. This is not dependent on Shh signaling.
Noggin mutants suffer severe reduction in sclerotomal derivatives.
Explain your reasoning. At least 6 different genes encoding ligands of the BMP family are expressed in the embryo during somite formation. Design an experiment to determine which of these BMPs regulate sclerotome differentiation during normal development. Explain how you would interpret the experiment. Either Noggin or Shh can induce presomitic mesoderm explants to differentiate as sclerotome in vitro .