The program will automatically convert this raw text into an organized formatted display. Simply insert as many dashes as neccessary in place of bases. This is handy when cutting and pasting data which includes numbers.
Most Browsers allow results to be cut and pasted in to other applications.
Our oligimers are typically 25 to 45 bases long and contain 1 to 3 base mismatching mutations. We try and keep the mutations no closer than ten bases of the ends of the primer. We use these primers in PCR to create a plasmid containing the altered gene. The above calculator renders finding approriate primers that bracket the desired mutation a trivial matter. Perhaps more importantly, since the answers can be cut and pasted, it avoids the possibility of transcription error manual calculations are prone to.