Toxin mass maps Venoms are typically made of some 50 different polypeptide toxins, most of which can be detected by LC-MS analysis. Our approach provides a unique fingerprint of each sample for species determination, detection of new compounds or batch controls prior to medical use. Glycosylation analysis LC-MS is ideal for the analysis of heterogeneous entities such as glycoproteins. After proteolytic digestion, a reversed phase column separates the different peptides. The mass spectra obtained allow characterisation of the different glycoforms.
Protein folding Even studies on folding pathways are possible for proteins.
Intermediates can be trapped with chemical reagents in a time-course experiment. Disulphide bond formation can for example be followed using alkylation of free thiols, enzymatic treatment and final LC-MS analysis. This includes a full mapping on our system of your crude sample with UV trace, TIC trace and mass spectra of all detected compounds. Single ion monitoring for a specific molecular weight is of course also possible. Again, thanks for visiting us.