Microscopy Proper adjustment of the microscope is essential. Slide shows neutrophils and gram positive cocci. Find the Gram positive and Gram negative rods in this field. Crystal violet precipitate crystal on gram stain. Reporting Results Use systematic, descriptive terminology to report gram stain results.
Occasional yeast is normal.
No yeast seen. Difinitive identification requires culture and biochemical testing. Carbolfuchsin is primary stain. Acid fast organisms resist decolorization with acid alcohol. After decolorization, methyelene blue is added to organisms to counterstain any material that is not acid fast.