There is more to effective sterilization than simply loading and running the autoclave.
Numerous factors affect efficacy.
Since autoclaves are high-pressure steam vessels, there also are safety considerations. Laboratory staff should be trained in proper and safe autoclave operation. Always refer to the manufacturer s operating manual for the specific recommendations on proper autoclave operating procedures. Time, temperature, and steam penetration are the main factors contributing to autoclave effectiveness. Whenever possible, you should use a verification process to determine the run time for your typical load.