Eggs are probably laid deep under rock cover, and thus extensive rock cover may be a requirement, but there are no data. Permanent water probably is not required. This species inhabits hilly, mountainous and canyon areas dominated by rocks. Snakes in the warmer southern part of the state will probably be active longer and earlier in the season than in the north. Lyre snakes lay eggs.
Nothing is known about disease, competition, or parasites.
Observations on the California lyre snake, Trimorphodon vandenburghi Klauber, with notes on the effectiveness of its venom. Klauber, L. M. 1940. The worm snakes of the genus Leptotyphlops in the United States and northern Mexico. San Diego Soc. Krutzsch, P. H. 1944.