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Nonlinear regression is an iterative procedure.
It then adjusts these initial values to improve the fit. Prism automatically provides initial values for each variable, calculated from the range of your data. If you enter a user-defined equation, you define the rules. See User-defined equations . You'll find it easy to estimate initial values if you have looked at a graph of the data, understand the model, and understand the meaning of all the parameters in the equation.