You can download our AAA Report Spreadsheet form and look at the cell formulas to gain additional insight into the way the spreadsheet operates. Adjacent hydrophobic residues are slow to hydrolyze and may result in low yields in addition to the formation of dipeptides which elute in unknown areas of the chromatogram. Asparagine is converted to aspartic acid and glutamine is converted to glutamic acid during hydrolysis. The conversion is quantitative. Methionine sulfoxide co-elutes with arginine.
References are available from the laboratory.
If you desire cysteine information, supply us with enough sample for two analyses. One analysis will be standard. In the other analysis we will hydrolyze the sample in the presence of DMSO to oxidize the labile residues present. We will then run a separate analysis on the DMSO hydrolysate for cysteic acid. For more accurate quantitation of specific amino acids, time series hydrolysis using times of between 12 and 96 hours can be arranged.