Store at room temperature. Place fixed, dry slides on slide rack in 95 degrees C oven and bake for 20 minutes. Remove slide from trypsin and immediately immerse in Fisher phospate buffer to stop trypsin action. Place slide cell side up on staining rack and flood with solution of 1 part Leishman's stain and 3 parts pHydrion working solution. Stain for 2 minutes.
Rinse slides thoroughly with distilled water.
Allow slides to drain, then place on 60 C slide warming tray until completely dry. Notes and Precautions 1. Trypsinization time may need to be varied depending on environmental conditions, material being banded, or typsin stock. Always stain one slide first and check banding quality before staining additional slides. Adjust trypsinization time if necessary.