Conductive film formation Particles of evaporated metal are deposited on the surface of the specimen and serve as nucleation sites which began to grow into islands of deposited metal. Islands ultimately grow and fuse into one continuous layer.
Noble metals tend to wet poorly, forming raised beads.
May be absent in deep recesses. Heat damage due to radiant heat from evaporation source. Lengthy setup and cleanup. A DNA spread prepared by Kleinschmidt technique and contrasted with uranium oxide. Large circular molecule is a plasmid standard at 5.3 kbp, the smaller molecule is psittacine circovirus at 1.5 kbp.