The experiment was carried out at the Munic MP tandem accelerator using 27 MeV protons. The outgoing particles were analyzed using the Z3D magnetic spectrometer. Resulting distributions were compared with DWBA calculations. We have PCR amplified the ITS region from 29 accessions of Gleditsia and one accession of its sister genus, Gymnocladus . We have obtained a 425 nucleotide sequence from 16 of these PCR products using cycle sequencing methods.
Phylogenetic analysis provides support for the hypotheses based on cpDNA data.
First, three main clades are apparent within the genus. Also, the two North American species, G. triacanthos and G. aquatica , again appear as closest relatives in all cladograms. We localized the gene, termed 4A11, in the Drosophila genome in order to study its relationship with nearby genes and determine its function. PCR amplification with 4A11-specific primers was used to resolve 4A11's cytological location to 72E4, close to the mutation bulge . This mutation causes blistering of the eye and a large number of irregular facets.