Microtomes are likely contaminated with paraffin residues. It contains mineral oils in an alcoholic medium. In principle it may be considered as a non-harmful cleaning solution. The final results in terms of brightness and contrast obtained with this technique are superior over those obtained with many alternative methods. The presence of this cation is required as it acts like a mordant between the acidic nucleus and the stain.
Modern technology allows a time and temperature controlled process.
Continuous motion avoids precipitation and guarantees a final end product of extreme stability. This stability guarantees consistency, brightness and clearness. Eosine Y is especially manufactured to match our Hematoxyline. Eosine Y is offered as an alcoholic or as an aqueous solution. The use of this Eosine and Scotch Buffer for coloring will guarantee consistent color development and will therefore contribute to extreme brightness and clearness of the stained tissue sections.