Staining corresponds to the future mid-hindbrain junction. Black arrow indicates eye field expression. Black double arrow indicates blastopore expression. Yellow arrow indicates future otic vesicle expression. Red double arrow indicates pronephric anlage expression.
Red arrow indicates pharyngeal arch staining.
White arrowhead indicates pronephric duct expression. Yellow arrow indicates spinal cord staining. Note periodic pattern. Red arrowhead indicates thyroid gland expression. White arrow indicates increased expression in the nephrostomes of the nephric tubules.