Under the hood, move the plantlets to tubes of pretransplant medium. Cap the tubes, and move them back into low light for 2 to 4 weeks. The shoots will continue to grow, but the plantlets will put most of their energy into producing roots. Once roots are visible, you want to move the plantlets from medium to soil. During this fourth stage, handle the plantlets very carefully.
Plants grown in vitro are very sensitive to any change.
Gently wash all the medium from the leaves and roots with distilled water, and transplant them to small, clean pots of African violet planting soil. To maintain the high humidity the plantlets are used to in the capped tubes, put the small pots in a larger container. Add 1 or 2 cm of water in the bottom of the large container, and cover it with cellophane wrap. Each day fold back more and more of the cover. In 1 or 2 weeks, the plants will be acclimated to room conditions.