Immediately after awakening and soon after heavy drinking or playing, your puppy will urinate. Right after eating, or if nervous, your puppy will have to defecate. Circling, whining, sniffing, and generally acting worried usually signals that defacation is imminent. Now that vaccinations are available for these diseases one would think they would no longer be a threat, but many dogs remain unvaccinated and continue to succumb to and spread these potentialy fatal illnessess. Don't let your Chihuahua be one of them.
Puppies recieve their dam's immunity through nursing in the first day of life.
This is why it is important that your pup's mother be properly immunized before breeding, and that your pup be able to nurse from its dam. The immunity gained from the mother will wear off after several weeks, and then the pup will be susceptible to disease unless you provide immunity through vaccinations. The problem is that there is no way to know exactly when this passive immunity will wear off, and vaccinations given before that time are ineffective. So you must revaccinate over a period of weeks so that your pup will not be unprotected and will recieve lasting immunity. I am guilty of breeding.