On 5 April orders were received to accompany General Hartwell on an expedition into the interior and was located at St. Andrews on 21 April when the cessation of hostilities was announced. Here the regiment remained until 7 May when it was ordered to Summerville, twenty-one miles up the Ashley River. On 19 May 1865 the headquarters was established at Orangeburg, South Carolina and the regiment was scattered in small detachments at various locations throughout the state. On 24 August 1865 the regiment proceeded to Charleston, and on 29 August was mustered out of the United States service at Mount Pleasant. On 25 September the entire regiment was paraded on the Common and disbanded.
Research indicates that most of the Ohio residents returned to their hometowns.
Some remained in the Boston area and others returned to South Carolina. Thomas Yoseloff, 1959. Fifty-Fifth Regimental Association. Record of the Service of the Fifty-Fifth Regiment of Massachusetts Volunteer Infantry. Ayers Company Publishers, Inc., 1991.