In general, it is not important to label systematically either the forward or the reverse primer. It is important to label the same primer if one wants to compare data between labs as both strands don't have the same molecular weight and one may obtain different artifactual bands with the forward versus the reverse primer being labeled. Table 1 lists marker quality in the last column. This refers to the optimum conditions to be used with the primers supplied. Please note, TMAC alters annealing temperatures.
References listed are the initial published reports for each locus.
The sizes listed in the table may be different from those published. In a few instances, the primer sequences are totally different from the published sequences resulting in PCR products in the 200-300 bp range. References Alexander, L.J., D.L. Troyer, G.A. Rohrer, T.P.L. Smith, L.B. Schook, and C.W. Beattie. Physical assignments of 68 porcine cosmid and lambda clones containing polymorphic microsatellites. Andersson Dear, D.V. and J.R. Miller.