If alternatives to animals are available, indicate why they cannot be used in this study. It is important to justify why certain species or strains must be used, particularly if they are wild or endangered species, e.g. non-human primates. The explanation of the numbers to be used should give an indication of the experimental design and the group sizes. The housing location is required so that space and handling requirements are adequate to the humane treatment of the animals. A list of searchable databases for alternative to animals may be obtained from the Library.
Many experimental procedures produce effects on animals beyond their original design.
Even a change of housing may cause sufficient distress to result in the expression of previously subclinical disease. In this section, any possible complications should be detailed so that attempts can be made to reduce the effects of the complications. A higher than expected incidence of complications may indicate a basic problem existing with the animals or with the techniques being employed. Detailed descriptions of all pursuit, capture, handling and chemical restraint procedures, and explanations of their appropriateness, are essential. Criteria used to assess suitability for release must be clearly stated.