Application rates vary but generally 1-2 billion nematodes are applied per acre. Insecticidal nematodes can be tanked mixed with certain products but this is not recommended because not all tank-mix combinations have been investigated for compatibility with nematodes. In addition, H . bacteriophora are more sensitive to insecticides than other nematodes thus its best not to mix these nematodes with insecticides. Instead, apply these nematodes at least 2 weeks before or after a pesticide application. Disposal Spray remaining nematodes and sprayer rinse water directly on the crop or dispose of on-site or according to federal and local regulations.
Mortality is temperature dependent.
It takes longer for the nematodes to kill grubs in cooler soil than warmer soil. However, nematode infested grubs should be visible within about a week after application. Dig up plants that you suspect are infested and look for the insects near the roots and crowns. Figure 4 shows a H. bacteriophora infested O. sulcatus pupae on the left and a healthy pupae on the right. Suppliers Refer to the Insect Parasitic Nematode Retail Suppliers page.