The new mutant allele may be located within the nadB gene but maps outside of the end points of the deletion intervals, for example in the same interval as allele 55. The black boxes represent the region removed by each deletion. The map position of several nadB mutations was determined by deletion mapping. The results are shown in the following table. Given these results, indicate the map position of the mutations.
Proteins encoded by the put operon allow cells to use proline as a sole carbon or nitrogen source.
A deletion map of part of the put operon is shown below. Based upon these results, what can you infer about the properties of the mutation. Propose a genetic recombination experiment to test your idea. There are a variety of ways you could test your idea. Based upon these results, what can you infer about the properties of the mutation.