The blood is allowed to spread along the width of the spreader slide and then in a rapid even motion, the spreader slide is pushed across the length of the original slide. The film is now air dried at room temperature. The preparation must now be fixed in methyl alcohol and stained with a Giemsa-type stain. This preparation, when dried and stained, can be examined microscopically under oil immersion. Other procedures Various culture procedures are used to increase the chances of detecting parasitic protozoan parasites.
Many culture media exist and various liquid and cell cultures are available for some organisms.
These procedures are most useful in identifying low level infections that might not be sufficient in number to be detected under routine procedures. Animal inoculation is also useful for this same reason. Specifics about animal susceptibility, route of inoculation, prepatient time, etc. Immunodiagnostic procedures . Specifics about the type of sample needed, collection procedures, and test sensitivity should be determined before considering this procedure.