The envelope of the virus will have two viral glycoproteins. Please limit your answer to the space provided. Messenger RNAs in eukaryotic cells are monocistronic. Viruses have evolved a variety of schemes for growth within this environment, given this constraint. The negative-sense VSV genome is transcribed into five, monocistronic, subgenomic mRNAs.
Methods are available for removing the nuclei from eukaryotic cells in culture.
These enucleated cells can still carry out their cytoplasmic metabolic functions for a period of time. However, the replication of the genome of these viruses differs drastically. In the table below, indicate which feature applies to the replication of the RNA genomes of which of these viruses. Replication Feature Poliovirus Rous sarcoma virus replication requires the use of a host cell tRNA as a primer. Indicate which of these strategies is employed in the case of each of the following viruses.