Triple Sugar Iron agar is a differentialmedium for gram-negative enteric organisms on the basis or theirability to ferment dextrose, lactose and sucrose and to producesulfides. Many culture media formulations combine the propertiesof selectivity and differentiation due to their inclusion of avariety of chemicals. Additionally, thismedium differentiates staphylococcal species on the basis onmannitol fermentation which is visualized by a color change inthe phenol red indicator. Hydrolysis of esculin to esculetin and dextrose. Differential isolation and presumptive identification of group D streptococci by black color around colonies.
Peptone agar with dextrose and ferrous sulfate.
Gram-positive organisms and other Enterobacteriaceae inhibited by the bismuth sulfite and brilliant green. Ferrous sulfate allows for the detection of H2S production. Selective for the isolation of Salmonella from stool. Salmonella colonies are black or green and may be surrounded by a black or dark brown zone with a green metallic sheen. For the cultivation of fastidious microorganisms and the determination of hemolysis reactions.