C. Immortalized cell lines . Remember a macrophage-like cell line is not a macrophage. Just remember, that his Hela cell is not the same as hers and vice versa. Defined apical and basolateral membranes. A plating medium for the detection of gram-negative bacteria.
Positive colonies are indicative of fecal contamination.
Blood agar plates to detect hemolysin egg yolk agar to detect phospholipases Casein agar to detect proteases. During the 1970's, bacterial transposons were characterized and turned into tools for mutagenesis in the late 1970s. For 20 years, transposon mutagenesis was the basic method of genetic analysis in this field. Eventually, it became accepted that cloning into E. coli K12 was safe. The concept of a reporter gene and designer transposons 1.