To Invoke the automatic sequence numbering and codon justification, Click mouse anywhere outside the Text Area. Use the mouse to Select any section of the DNA sequence. See Below for more details. Simply insert as many dashes as neccessary in place of bases. This is handy when cutting and pasting data which includes numbers.
Most Browsers allow results to be cut and pasted in to other applications.
Our oligimers are typically 25 to 45 bases long and contain 1 to 3 base mismatching mutations. We try and keep the mutations within ten bases of the ends of the primer. We use these primers in PCR to create a plasmid containing the altered gene. The above calculator renders finding approriate primers that bracket the desired mutation a trivial matter. Perhaps more importantly, since the answers can be cut and pasted, it avoids the possibility of transcription error manual calculations are prone to.