Therefore when we describe the specific DNA binding sequence for a transcription factor we refer to the non-variable part of the binding sequence, that is, the transcription factor consensus sequence. Transcription factor ODN decoy approach . The basic theory behind the transcription factor ODN decoy approach involves flooding the cell with competing synthetic, transcription factor-specific consensus sequences. Transfected at high concentrations these decoys have been reported in the literature to completely block transcription factor function. Advantages and disadvantages of the ODN decoy approach for studying cellular gene expression.
ODN decoys offer a means of specifically inhibiting transcription factor function in living cells.
Inexpensive compared to other more classical methods of investigating gene expression such as chloramphenicol acetyltransferase and luciferase constructs in promoter-reporter gene transfection experiments. Allows for investigation of both endogenous and pathological gene regulation Proven to be highly effective and selective within in vitro experiments. Easy to use. An emerging technology that has not yet been fully characterized Issues of decoy synthesis. High levels of purity and stability required.