The predicted molecular weight of phosphofructokinase was determined by translating the protein DNA into amino acids using the MacDNAsis program. The number of amino acids played an important role in predicting the molecular weight at 85556.55 daltons. A hydropathy plot was used to determine whether phosphofructokinase is an integral membrane protein or not. The Kyte and Doolittle plot shows the hydrophobicity of the protein, with the positive values indicating more hydrophobic regions. The hydropathy plot generated under the parameters of Kyte and Doolittle using MacDNAsis indicates whether a protein is an integral membrane protein.
Positive numbers signify hydrophobic regions with more hydrophobic regions having higher values.
The Hopp and Woods plot is used to determine what portion of the protein is more antigenic and can be used to make a peptide. This peptide could then be used to make a monoclonal antibody against the peptide. Positive values indicate more antigenic areas of phosphofructokinase. These regions are directed away from the hydrophobic phospholipid bilayer of the membrane and can be used to generate peptides against which monoclonal antibodies can be made. The Chou, Fasman, and Rose plot shows the predicted secondary structure of phosphofructokinase.