Part II Problems and Short Essays 1. Professor Essigsauer is back in the lab playing around with paper chromatography this time. Unfortunately, his impatience is getting in the way of a more systematic approach. Provide a clear explanation for your recommendations. Feel free to use illustrations to support your explanations.
Progress depends on new techniques, new discoveries, and new ideas, probably in that order.
Sydney Brenner Analyze each of the three articles you have studied so far this semester in the context of Brenner's quotation. Department of Biochemistry, Kansas State University, Manhattan, Kansas 66506 Hemolysates from the adult bullfrog, Rana catesbeiana, show an unusually high degree of cooperativity of oxygen binding with Hill coefficients greater than 4. The principal components of the tetrameric hemoglobin, B and C, do not show this high cooperativity when isolated, but it reappears when the components are mixed. Sedimentation velocity measurements show that the unusual behavior results from mixed association of components B and C to form complexes larger than tetramers. Computer simulation of the sedimentation behavior of mixtures of deoxygenated B and C components shows that the gradient profiles can be satisfactorily described in terms of an equilibrium between the B and C tetramers and a BC 2 trimer.