The Gluconeogenesis Pathway 1. Gluconeogenesis utilizes glycolytic enzymes, but must replace hexokinase, PFK, and PK because these enzymes direct the pathway toward glycolysis 2. Phase II Activated carboxyl group is transferred from carboxybiotin to pyruvate 3-step reaction that forms oxaloacetate c. Acetyl-CoA regulates pyruvate carboxylase a.
Oxaloacetate synthesis is an anaplerotic reaction that increases CAC activity b.
If CAC is inhibited, oxaloacetate instead undergoes gluconeogenesis 6. PEP carboxykinase catalyzes the GTP-driven decarboxylation of oxaloacetate to form PEP and GDP 7. Gluconeogenesis requires metabolite transport between mitochondria and cytosol a. Generation of oxaloacetate form pyruvate or CAC intermediates occurs only in the mitochondrion, whereas the enzymes that convert PEP to glucose are cytosolic b. Hydrolytic reactions bypass PFK and hexokinase a. The Cori Cycle 1.