Add 20 live Artemia into each of the above salt mixtures. Place all the bowls on the tray and leave it on the back shelf. Observe and record the survival of Artemia in each of the salt solutions. Present it in a tabular form. For those who are curious about how things work in Artemia .
Neutral red is a particulate stain.
In 200X sea water, add methylene blue and observe the next hour or day. Rinse the animals with fresh solution and observe under a dissecting scope. Methylene blue is a cationic dye. It will accumulate where there is an accumulation of charge.