Keep incompatible gas classes stored seperately. Oxygen and nitrous oxide cylinders must be separated from flammables or fuel gas cylinders and combustible materials by a minimum of 20 feet, or by a 5 feet high barrier with a fire rating of at least one half hour. Segregate gas storage from all other chemicals. Do not expose cylinders to an open flame or to any temperature above 125 degrees Fahrenheit. Attach the regulator securely before opening the valve.
Open cylinder valves slowly.
Do not use a wrench to open or close a hand wheel type valve, if it can not be operated by hand , it should be repaired by the vendor or qualified individuals. Spring loaded pressure relief regulators should be used. When used with hazardous, flammable, or toxic gases, the valve should be vented to the fume hood. These substances may be reactive with some gases such as oxygen. Regulators used with oxidizing agents must be carefully cleaned to avoid the possibility of explosion due to contact of the gas with any reducing agent or oil.