Susceptible check Wisconsin SMR 18. Resistant check Gy 14. Resistant and susceptible checks are sown in row 6. The flats are placed on a heated germination bench. Newspaper on top of the flat prevents cooling by evaporation.
Newspaper is removed when seeds germinate.
Plants are rated as resistant, intermediate, or susceptible 6-8 days after inoculation. Resistant plants develop necrotic tan lesions. Intermediate plants have tan lesions with a yellow green border. Susceptible plants have elongate irregular lesions with yellow margins. Resistant, intermediate, and susceptible plants can be transplanted to steam sterilized soil.