In experiment 4 students isolation the V. fischeri DNA and move the bioluminescence genes into E. coli . In the process many modern techniques used in genetic engineering are taught. Experiment 4 - DNA sequencing A didactic, computer exercise is used to teach some of the concepts of DNA sequencing. In the future we hope to actually have students perform sequencing in the lab. Experiment 5 - Transport Examination of the transport of the amino acid Leucine into the bacterium Pseudomonas fluorecscens in the presence and absence of various inhibitors.
Radioactivity is followed using scintillation counting.
The purified protein is analyzed for purity and a western blot is performed. Experiment 7 - Heat shock Investigation of the heat shock proteins of E. coli . Please note that all information on this website is subject to our disclaimer .