Falcon 2054 tubes come with caps. After initial setup, the minimum volume can be reduced to 100 ul at the previous cell concentration. The minimum recommended volume is 250 ul. The minimum volume that can be run is about 300 ul. Sort Media Sorted cells ride in droplets composed of sheath fluid on their way to the sort collection tube.
Most cell types tolerate exposure to the sheath fluid preservative and thrive after sorting.
In experiments where cells may not tolerate exposure to the preservative in the sheath fluid, we recommend substituting cell growth media for the standard sheath fluid. In order for us to make the sheath fluid subsitution in the instrument, the lab requesting the sort should bring a minimum of 1.5L of growth media to the Flow Cytometry Facility the day before the sort. Sorting for more than one hour will require a larger volume of growth media. Because of spectral overlap between certain fluorochromes, samples must be provided that have been stained positive with each of the single fluorochromes that overlap. For an explanation and procedure, see Mario Roederer's website on compenstation .