Sometimes when a female and her mate enter the water, other males see them entering and attempt to force the male from her back. If this is accomplished, another male will take his positon and fertilize the next clutch eggs. While most frog species lay their eggs directly into the water, A. callidryas lay theirs on the underside of leaves that hang over bodies of water. When the clutches of eggs have developed into tadpoles, which occurs very quickly, the tadpoles swim around within their eggs until the egg ruptures. The rupturing of all the eggs in the clutch occurs within a one minute time period, and the fluid released from the ruptured eggs helps to wash all the tadpoles down the leaf and into the waiting water below.
Reproduction is a very strenous activity for A. callidryas .
Helix, C. 1997. Behavior Some believe that the bright, red eyes of A. callidryas act as a form of defense termed startle coloration. Red-eyed tree frogs are nocturnal and rest during the day. If a predator were to happen upon A. callidryas , the frog would awaken, and its eyes would pop open abruptly. The sudden brightness of their red eyes might startle the predator enough to give A. callidryas the mere seconds necessary for the agile frog to jump to safety.