Non-viral DNA vectors can be delivered to various tissues using a growing variety of methods. We are attempting to deliver the vectors to mice using cationic liposomes, in collaboration with a local biotechnology company. Some diseases of immediate interest to us are cystic fibrosis, hemophilia, and cancer. Isolation of human sequences which replicate autonomously in human cells. Improved EBV shuttle vectors.
Replication initiates at multiple locations on an autonomously replicating plasmid in human cells.
Replication control of autonomously replicating human sequences. Nucleic Acids Res. Analysis of the autonomous replication behavior in human cells of the dihydrofolate reductase putative chromosomal origin of replication. The replication behavior of Saccharomyces cerevisiae DNA in human cells. Autonomous replication in human cells of multimers of specific human and bacterial DNA sequences.