With adequate sample preparation and amount, we routinely sequence 25 residues and have identified over 70 residues in a single run. We also offer protein digests, electrophoresis, electroblotting and desalting, specialty sequencing and database searching. Contact Us Prior to Sample Preparation New customers should discuss their requirements with our experienced protein sequencing specialist Wilson Yan at 514-0101 x1. This avoids inappropriate methods and the loss of precious samples. Protein Sequencing Sample Preparation Protein samples are typically submitted either in solution or as a PVDF blot.
Purity is very important.
Your protein should be free of other proteins and low molecular weight contaminants. For samples submitted in solution, we suggest that you never dry the sample completely. These samples must be accompanied by evidence of purity. Detergents should be avoided, as they reduce binding capacity during desalting, but some are acceptable in low concentrations. See Acceptable Concentrations of Common Detergents.