The polyMICA TM Detection kit is intended for the immunohistochemical detection of antigens in human tissue sections which have first been labelled with primary antibodies raised in mouse and sheep. The principal of this system is the sequential binding of two mutually attractive antibodies. This mutual attraction increases the valancy of the antibodies when compared to traditional multi-step labelling methods, thereby increasing the sensitivity. The enhanced sensitivity of polyMICA TM enables the detection of many low level target antigens, as well as the possibility of diluting primary antibodies 10-20 times further, or greater. As the polyMICA TM system is biotin free, the non-specific staining of endogenous biotin is avoided.
Reagents are ready to use and are presented in kit format.
The kit includes a primary antibody diluent and chromogen.