There was an immediate and marked difference in the appearence of the viscera of these dogs both grossly and ultrastructurally. Based on slice studies we expect these results to be even better than was achieved with high rates of rewarming. We are all in the same boat in terms of high glycerol concentration exposure of our patients at relatively high temperatures. Incidentally, this is why freezing rate is so critically important to survival of cells frozen with CPAs. As the ice forms it freezes out as PURE water and this causes a rapid rise in cryoprotectant concentration.
Reduced temperatures mean reduced toxicity.
There is just too much heat to move. When I was at Alcor and more recently at BPI I examined low concentration approaches to brain cryopreservation. Damage was far worse, as can be seen the EMs published in CryoCare report. This does not make me happy, and, even having paid such a price, we are still experiencing a lot of cryoinjury. While it is certainly true that just because ultrastructre isn't there when we look for it with today's techniques it may be found by tomorrow's.