Many histological stains fail to react with the specimen in the epoxy media.
Using our E.T.S., sections of tissue embedded epoxy can be stained by a very simple method. This polychromatic staining solution can be used for staining sections of epoxy-embedded specimens prepared for electron microscopy examination, e.g., glutaraldehyde and osmium fixation. The sections are placed on a glass microscope slide and dried on a hot plate. A drop of E.T.S. is added to cover the section on the warmed slide, and the slide is put back on the hot plate until a silver rim is formed. Any excess stain is removed with distilled water from a squeeze bottle, the slide is allowed to dry, and the sections are covered with a cover-slip.