However, we have continued parallel experiments in our estrogen-treated animals on alternative tissues that may be responsive, in particular liver, which is present in relatively larger quantities and is known to respond to estradiol. We hope that our encouraging efforts with differential display will provide better liver markers. Our problems with limited amounts of uterine tissue to examine methoxychlor responses would then be solved. In our original proposal we only presented lactoferrin mRNA induction by methoxychlor at a single time point and concentration with a single technique, in situ hybridization. One page printout of poster presented at 1998 Endocrine Meeting in New Orleans.
Reference is below.
The dose and time dependent response to methoxychlor supports its action through receptor binding and saturation. However, in general ERa appears so far to be very much like ERb in its ligand-binding domain specificity. Data were presented comparing the binding affinities of these splice variants to other estrogens, including methoxychlor. No steroid-binding characterization has yet been reported for any of the 12 possible heterodimers. Combined, these two new reports raise the testable possibility of altered ligand specificities with these heterodimers.