Specific work includes the construction of high-resolution maps of human chromosomes 5 and 16 and associated informatics and gene discovery tasks. All available loci continue to be incorporated into the map. Refinement of BAC cloning procedures for future production of chromosome-specific libraries. Successful partial digestion and cloning of microgram quantities of chromosomal DNA embedded in agarose plugs. Efforts continue to increase the average insert size to about 100,000 bases.
SASE data is deposited in publicly available databases to allow for wide distribution.
Finished sequencing is prioritized from initial SASE analysis and pursued by parallel primer walking. Accomplishments SASE sequencing of 1.5 million bases from the p13 region of human chromosome 16. Discovery of more than 100 genes in SASE sequences. Generation of finished sequence for a 240,000-base telomeric region of human chromosome7q. From initial sequences generated by SASE, oligonucleotides were synthesized and used for primer walking directly from cosmids comprising the contig map.