Many of the hazards encountered during the use of the centrifuges in this department are associated with the nature of the materials used rather than the machines themselves, and the appropriate COP should be consulted in each case. Possible exceptions are the creation of aerosols during centrifugation, and the very large amounts of Ethidium bromide used in some density gradient experiments. Rotors Rotor failure is a major hazard associated with the use of high-speed centrifuges. All personnel wishing to use centrifuges other than the low speed centrifuges and the micro-centrifuges in individual laboratories must first obtain obstruction and authorization from a senior member of Departmental staff. The manufacturer's recommendations, especially with regard to sample balancing, maximum rotor speed and speed reduction for samples of high specific gravity, must be strictly adhered to.
Operation manuals are available at each machine.
It is the responsibility of the user to check on the condition of rotors and centrifuges before each use, and to report any real or apparent defect immediately. If a rotor does break the centrifuge may suddenly jump several feet across the room and can easily fracture limbs. Therefore do not lean on the centrifuge or use it as a table when completing the log book. 4.2 Autoclaves Never use an autoclave without prior instruction from an experienced member of the Department. Only named persons can operate specific autoclaves.