Sequence Analysis Sequence analysis is carried out on three Applied Biosystems 377 gel DNA Sequencers and a new capillary ABI 3700 DNA Analyzer . The electrophoretic data are returned as a computer-readable sequence file via ftp and by request a four color eletropherogram plot which may be picked up at BCMM 305.
Quality read length is slightly less with the capillary instrument than with the gels.
Plasmid DNA B . The quality of your sequence will be directly related to the quality and accuracy of the quantitation of your DNA template. Excess salt and ethanol have been shown to inhibit the DNA polymerase. Template preparation for automated sequencing Concentration of template should be based on OD 260 and then Check the quality and verify the concentration of your DNA by gel electrophoresis. Avoid multiple Thymidine residues on 3' and 5' ends.