A typical laminar flow hood. There is a work surface surrounded by plastic sides and a plastic top. Filtered air enters the work space from the back. The incubator is set to 37 degrees centigrade. An additional consideration is the buffering of the culture fluid.
Most culture media contain bicarbonate and the buffering system is carbon dioxide - bicarbonate.
The incubator Fig. 2. A typical incubator for cell culture. It's simply a box whose internal temperature is controlled by a precise thermostat and a heater. The tanks to the right of the incubator are carbon dioxide tanks used to provide the carbon dioxide for cell culture. The inside of a cell culture incubator.