Resistant and susceptible checks are sown in row 6. The flats are placed on a heated germination bench. Newspaper on top of the flat prevents cooling by evaporation. Newspaper is removed when seeds germinate. Plants are rated as resistant, intermediate, or susceptible 6-8 days after inoculation.
Resistant plants develop necrotic tan lesions.
Intermediate plants have tan lesions with a yellow green border. Susceptible plants have elongate irregular lesions with yellow margins. Resistant, intermediate, and susceptible plants can be transplanted to steam sterilized soil. Inoculate with mist sprayer under newer leaves.