If the analogue, at an equal concentration, was added instead, no migration occurred. If the analogue was added first, then IL-72, there was no migration. It is hypothesized that the situation is similar in DTH cells. You set up an in vitro study to test the DTH migration response to IL-72 and its analogue. 1000 cells per well with a membrane barrier.
Positive migration is scored by cells passing through the barrier to the other side.