The only intelligent method of determining the dosage requirements is to test a sample of the bio mass. BioMagic recommends your local Test Lab for the tests to determine the basics of your bio mass. After the lab establishes the parameters of the bio mass, a specific controlled test on a 50 gallon or so of the bio mass should be conducted the verify the assumptions made based on the lab test report. Only facultative anaerobes, that are the natural bacteria from animal and human digestive system can do so. Facultative anaerobe bacteria can use dissolved oxygen or BioMagic as a source of oxygen.
Pure aerobes require dissolved oxygen provided by aeration.