Audubon wrote that the sight of the spring afforded him pleasure sufficient to counterbalance the tediousness of his daylong journey in a carriage drawn by Indian horses from the Bulow plantation. Swimming is not allowed in the spring run but anglers will find bass and bream in abundance. A Florida freshwater fishing license is required for persons 16 years of age or older. DeLeon Springs provides access to Lake Woodruff National Wildlife Refuge, where canoeists may explore 21,500 acres of lakes, creeks and marshes.
Maps are available at the park concession, as are canoe and paddleboat rentals and snacks.
Scuba diving is limited to open-water instructors and six students. A certification check and Instructor Permit are required. A half-mile nature trail meanders through floodplain forest, where a variety of plant and animal life awaits. The trail is extended by Wild Persimmon Trail, built by Florida Trail Association. Picnickers can gather under the live oak trees that grow around the spring.