If you are proposing to use HPLC for a particular analysis, give the column, detector, solvent, and any other parameters that you will use. If the analysis has been published, give a literature citation. Once again, you must be specific. A well thought out budget is essential. It is important not to cut costs to the point where the project cannot be accomplished.
Reviewers are also particularly adept at spotting budgets that are padded.
Adhere to proposal guidelines. Study the proposal guidelines as to format and length and adhere to them to the letter. Your proposal can be removed from competition for being too long, past the deadline, or for failing to answer key questions required by the granting agency.