This result demonstrates that the cannabinoid receptor can modulate the function of other neurotransmitter receptors. We are currently studying the mechanism responsible for this unique ability of the cannabinoid receptor. Calcium currents were recorded using the whole-cell patch clamp technique. A second application of somatostatin also had no effect on thecalcium current. Expression of the human CB1 cannabinoid receptor abolished the effect of somatostatin.
Number of neurons tested is indicated.
In another project in my lab we have found that another G protein coupled receptor, the human gonadotropin-releasing hormone receptor, can inhibit a type of potassium current. In collaboration with research scientists at the University of Chicago we have found that a mutant human gonadotropin-releasing hormone receptor has lost its ability to inhibit this potassium current. This mutant gonadotropin-releasing hormone receptor was found in patients with idiopathic hypogonadotropic hypogonadism. The techniques used in our research represent powerful and sensitive means by which to assay the function of both wild type and mutant neurotranmitter receptors. The long-term goal of the lab is to uncover the basic mechanisms of neuronal communication.