Good ts mutants that involve DNA synthesis. 1.DNA replication is semi-conservative. DNA as replicative intermediate 3.No Okazaki Fragments found. Possibly type 1 and type 2 replication. Pre-terminal protein linked to 5' deoxy cytidine of the terminal inverted repeat through a phosphodiester bond of serine hydroxyl group and 5'hydroxy terminal of deoxycytidine residue.
Proteolytic processing occurs during viral maturation and is a viral function.
Replication in vitro requires three viral proteins, the DNA pol that forms a heterodimer with the pre-terminal protein. Viral replication utilizes a viral encoded polymerase. Site directed mutagenesis in this area destroys the ability to replicate. Thus Tp protein acts as primer for adenovirus replication. A host encoded non specific DNA binding protein stimulates the replication.