Calculate the pH of a buffer that was prepared by dissolving 0.02 moles of NaHCO 3 in slightly less than a liter of water. The pKa of H 2 CO 3 is 6.1. Dianne Beatty was taken to the hospital in a coma. Calculate the pH of Dianne Beatty s blood based on the above blood gas analysis. Extra bicarbonate was being excreted into the urine.
Proteins are particularly important buffers in interstitial fluid.
TCO 2 measurements serve as a good indicator for blood HCO 3 - . Vomiting can result in metabolic acidosis. The amino acid histidine could be used to prepare a buffer that would be effective at pH 6.5 because the imidazole group in the sidechain has a pKa of 6.0. Calculate the moles of each ionic species of histidine that would be required to prepare a 0.05 M histidine buffer at pH 6.5. Metabolic alkalosis 18.