Some equipment requires detailed instruction while others are intuitive. Removing samples and paraphernalia when finished is important to keeping the area manageable. Rotors should be rinsed with tap water and inverted for drying. If you give a wipe while waiting for spins or data, you have made this place better for us all. This allows for tracing even minor problems to their source.
Recent users can help pinpoint a problem by describing any symptoms that they observed.
The answer to this is complex. If you find the equipment is difficult to get on, or you have to use it in sequence with experimental parameters, please plan and reserve only the time you know you need. If you are signed up for a time and do not start within 20 minutes, your reservation is forfeited, and the machine is considered available. If there are consistent conflicts, please check the shared equipment list for alternatives. If there are none, talk with the Operations Manager or your P.I. so that we can consider alternatives or acquisitions.