To date, we are unable to achieve specific staining.
Most references give little information regarding incubation times, etc.
Thanks in advance. Dear Victoria, Many antigens can be stained in JB-4 or Lowicryl without etching of any kind. Many etching procedures are bad for antigenicity, so you might try seeing what you get without etching at all. And, you did not say if you were amining for light microscopy or em. But if you are working in light microscopy and you find that you are unable to get good staining from Lowicryl or JB-4, you might try the removable methacrylate system.