The phycocyanin-labeled antibody reagents are optimally excited at 620 nm, however they are adequately excited with a dye laser tuned to 600 nm. Their emission occurs at 655 nm. Similar to the phycocyanin probes, the allophycocyanin-labeled reagents are optimally excited at 620 nm or higher, however, a dye laser tuned to 600 nm will excite this dye adequately. Their emission occurs in the range of 655 to 660 nm. These reagents may also be used in conjunction with other fluorescent tracers for double or triple-labeling techniques.
Reprints are available upon request.
The highly efficient and specific affinity which avidin displays for biotin and biotin-containing macromolecules, has been utilized extensively to identify artificially biotinylated biomolecules in a myriad of systems. Labeling avidin or streptavidin with a traceable substance provides the simplest form of the Avidin-Biotin Complex detection system. Phycoprobe of the PE type is recommended as a substitute for fluorescein-based systems. The removal of the carbohydrate side chains of avidin considerably improves the quality of avidin as a tracing compound. The phycoerythrin deglycosylated conjugate listed on this page under the trademark of Deglyco-Avidin, in most cases, will produce a better and cleaner signal than those based on native avidin and streptavidin.