The success of chilled semen transport rests very heavily with the veterinarian attending the recipient mares. It will be necessary for that veterinarian to predict the time of ovulation at least 48 hours in advance so that the semen can be collected and transported to the recipient mares. It is then important that the mares be inseminated as close to ovulation as possible and as soon after semen collection as possible. An in-season mare will be required for collection of the semen. The stallion is mounted on the mare and the penis diverted in to an artificial vagina for semen collection.
Most stallions tolerate the procedure quite well.
The semen is evaluated and diluted with semen extender. The extender will help prolong the life of the semen. The recipient mares should be inseminated as soon as possible after the arrival of the chilled semen. Your veterinarian may decide to divide the ejaculate and inseminate several mares from the same collection but should aim to inseminate a minimum of one billion live progressively motile sperm into each mare. Depending on the ejaculate and the stallion, up to 5 mares may be inseminated from a single ejaculate.