While this is considerably less sulfoxidation than observed in rat jejunum, slower perfusion flow rates at lower cimetidine concentration are projected to produce a greater extent of lumenal metabolite. Inhibitors of Cimetidine Sulfoxide Appearance in the Rat Jejunal Lumen. Subsequent to identification of cimetidine sulfoxidation, potential inhibitors were selected from products of intestinal sulfur-containing amino acid metabolism. Co-perfusion of 0.4 mM cimetidine with 2.5 mM glutathione reduced the appearance of cimetidine sulfoxide in the rat jejunal lumen. While the DIDS results suggest that metabolite export might be blocked, lumenal methionine sulfoxide was not detected from cimetidine co-perfusion with methionine.
More mechanistic studies are in process.
Center for advice and clinical study support. This work was supported by an Upjohn Research Endowment Award. D Thesis, University of Michigan, 1992.