Image1 and Image2 must be the same data type but do not have to be the same size. Check Create New Window and a new image or stack will be created to hold the result. Otherwise, the result of the operation replaces some or all of Image1 . In these examples, the source and destination have inverted LUTs so zero pixels are white. Operations on images with non-inverted LUTs, and RGB images, will not produce the same results.
Removes smooth continuous backgrounds from gels and other images.
Based on NIH Image Pascal code written by Michael Castle and Janice Keller of the University of Michigan Mental Health Research Institute. The Rolling Ball Radius should be at least as large as the radius of the largest object in the image that is not part of the background. Repeat Command Reruns the previous command. The Undo and Open commands are skipped.