To get a permanent record of the gel, Xerox the ponceau stained blot. Do the incubation in a sealameal bag for 1 hr at room temp on a rocker. Can go overnight in the fridge. Block again 30 sec in blotto. Add developing solution and incubate on rotator in box lid until bands come up.
Reagents are light sensitive, so it's best to cover with aluminum foil while developing.
Can develop up to several hours, although it's mostly done in an hour. The background fades when the blot dries, so don't be afraid to let the background go up. Rinse in water and let dry between Whatmann sheets. Bands fade in the light, so keep it in your notebook. Mix for 1 minute, then working quickly, dab the blot against Whatmann paper to remove excess liquid while leaving the blot moist, wrap the blot in saran wrap, and expose serially to film in a cassette for 30 seconds, 1.5 min, and 5 minutes.