The Kyte and Doolittle plot shows the hydrophobicity of the protein, with the positive values indicating more hydrophobic regions. The hydropathy plot generated under the parameters of Kyte and Doolittle using MacDNAsis indicates whether a protein is an integral membrane protein. Positive numbers signify hydrophobic regions with more hydrophobic regions having higher values. The Hopp and Woods plot is used to determine what portion of the protein is more antigenic and can be used to make a peptide. This peptide could then be used to make a monoclonal antibody against the peptide.
Positive values indicate more antigenic areas of phosphofructokinase.
These regions are directed away from the hydrophobic phospholipid bilayer of the membrane and can be used to generate peptides against which monoclonal antibodies can be made. The Chou, Fasman, and Rose plot shows the predicted secondary structure of phosphofructokinase. Types of structure are displayed above the graphic. As pictured in Figure 4, the secondary structure of phosphofructokinase includes six turns and many helices, sheets, and coils. The secondary structure can also be view in 3D with the Rasmol Image .