To address these issues, high throughput HPLC methods are needed. This presentation describes the strategies used to develop fast, generic high throughput HPLC methods. Our approach to decrease the typical HPLC run time and solvent consumption involves decreasing column length and diameter, reducing gradient time, and increasing column flow. Reductions in column length and increases in flow usually result in a loss in efficiency, and it is this loss that ultimately limits the method's resolving power. To achieve reductions in run time and mobile phase consumption, while maintaining resolution, other parameters, such as particle size, temperature effects, column types, scaling the flow rate, and scaling the gradient time were also studied and discussed in this presentation.
Moisture content is one of the primary chemical parameters measured.
There are a wide variety of methods for the determination of moisture in food and the method selected depends on the type of food being analyzed. The technique we selected to automate is for the determination of moisture based on oven drying. The method employs a convection vacuum oven. The weight change in the sample is reported as moisture loss. This technique was an ideal candidate for complete automation since we analyze over 5,000 samples a year.