The cuts may be opposite each other on the DNA backbone or staggered.
Opposite cuts produce blunt ends, while staggered cuts produce sticky ends.
Because restriction endonucleases recognize specific sequences and cut DNA to produce sticky ends, they are useful for gene splicing manipulations. Restriction enzymes are named after the organism they are isolated from and usually the temporal order of their isolation. For example Eco RV is the fifth reported from E. coli. Protocol I. Restriction Digestion of Lambda DNA Identify and organize the following reagents on ice. Then, very carefully pipette each in order into a 1.5 ml Eppendorf tube.