The fluoresent in situ hybridization procedure makes it possible to test the expression or splicing of RNA from each allele or a specific gene or transgene within the cell nucleus. Targets for detection may include messages derived from cellular genes or inserted genes which are known to require defined expression or splicing patterns for proper functioning. Specifically, it may be used to assess whether a distinct gene is active, as well as quantitation of that acivity. In addition, particular alleles of genes may be distinguished to determine which homolog is the active form, or whether RNA from each homolog is being spliced and transported. The method allows for visual discrimination of normal and defective processing within the cell.
Previous methods utilize molecular biology and biochemical techniques for detection.
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