I'm having problems with LR White sections. Dehydration is from buffer through ethanol series to n-butanol. LR White and tissue are hardened by heating at 55 degrees C for 24 hours in a Beem capsule. Jung 2040 using a carbide tungsten blade at 3 microns. Sections are placed on a drop of water before air drying.
Problems start at the staining.
The staining looks great, but I can't photograph wavy sections. The protocol I'm trying to use was designed for GMA, not LR White, but I'd hoped to just substitute the less toxic choice. Any help is greatly appreciated. If heated in a drop of water they are likely to stretch better too. This may be the answer to those wrinkles too, but some very thick plant cell walls can be difficult in that respect.