In the center is the target Linbro plate, and a rack with rinse bottles. On the left are coverslips, laid out in a rack ready to receive droplets. Execution Solutions are aspirated in a disposable tip, and transferred to the central Linbro plate and mixed. Protein for the whole run is picked up into a fine 2-bore tip. Next the other bore of the 2-bore tip is used to pick up around 1 microlitre of reservoir solution and transfer it to the appropriate coverslip.
Protein is dispensed simultaneously.
The coverslips are placed on the Linbro plate by hand.