Figure 1 summarizes this quality control protocol. Also to measure the background rate of mutation, uncut plasmid DNA was transformed into E. coli . This number corresponds closely with that for the solution purification and indicates that very little contamination derives from the agarose. In addition, the transformation efficiency for DNA purified from the low melting point gel and religated was better than DNA purified from solution. Purification of a widely sized range of fragments.
Recoveries decrease for larger and smaller fragments as indicated on the gel.
Gel purification of DNA fragments with different sizes Researchers purify DNA fragments covering a wide range of sizes. To test the effectiveness of gel isolations over a wide size range, we performed two tests. All the fragments were successfully recovered. Although fragments larger than 9kb may be extracted from Promega's Low Melting Point Agarose used together with Wizard PCR Preps, the yield decreases with larger size, and the linearized DNA can shear during the mechanical process. Yields are dependent on fragment size.