The P6 reporter reproduces the wildtype muscle expression pattern. The Gal4B2.1 transgene is identical to Gal4BII except that it is missing the downstream intronic region. Neither Gal4BII nor Gal4B2.1 showed expression in the larval brain. However, P6 shows limited, low level expression in the brain. In the first panel an adult P6 transformant was sectioned to display the head, thorax and abdomen.
Expression is obvious in the thorax.
The remaining panels are magnified views of sectioned thoraces. The last two panels are stained thoraces of Gal4B2.1 transformants. In these, the tissue not stained by Xgal has been visualized with a safranin counter-stain. The last section is near the center of the animal. To determine relative expression levels the samples were stained together.