The capacity of the cells to repair UV-induced DNA damage will be indicated by restored reporter activity. Unirradiated DNA construct will be introduced into cells and serve as control. We hypothesize that AOM mediates this induction by increasing the expression of EGF receptor. We will test this hypothesis by determining whether AOM does alter EGF-receptor expression in colonic mucosa. Protein Phosphorylation and Lymphocyte Polarization Circulating lymphocytes must polarize in order to migrate into solid tissue.
Polarization aids both normal immune response and the invasion of malignant lymphocytes.
We hypothesize that polarization is regulated by changes in protein phosphorylation. If a drug produces a change in polarization, we will perform immunofluorescence analysis to determine which cytoskeletal components are most affected. Return to referring page.