Isolation of mutations in Notch which affect its ability to suppress cell fates from the membrane and the nucleus. This function of Notch relies on its interaction with a ligand encoded by the Delta gene, and is mediated by the the intracellular domain of Notch. This project will involve the mutagenesis of the intracellular domain of Notch in vivo and the characterization of the mutants that are obtained in the hope of pinpointing the residues of the intracellular domain required for this novel Notch signalling activity. These will be picked up, DNA will be made from them and the intracellular region of the receptor sequenced and scanned for alterations in the aminoacid sequence that would be responsible for the loss of the dominant activity. Science 284, 770-776.
Notch is required for wingless signalling in the epidermis of Drosophila .
Cell 79, 259-272. A functional analysis of Notch mutations in Drosophila. Genetics 147, 177-188 4. Repression by Notch is required before Wingless signalling during muscle progenitor cell development in Drosophila . Current Biology 9, 707-710. 5.