You should shift gears, pedal, and test the brakes. If you ride clipless pedals, check your cleats for tightness and debris, and lube the mechanism in the pedal. Check any lights, racks, handlebar bags, seat bags, or other items to make sure they are properly attached to the bike. Remove any trash or unused food from your bags, and check the gear you may be carrying in them. Make sure you have the proper tools with you for the ride you are planning.
Most riders carry some tools, or at the very least a pump and patch kit.
The Wrecking Crew recommends taking MORE rather than less if your ride is of an extended duration or distance. Find out what tools your partner is carrying if you are riding with someone. Look for the Fat Tire festival in your state.