This web page was produced as an assignment for an undergraduate course at Davidson College. MacDNAsis Analysis of DNA Polymerase MacDNAsis is a computer program that allows for analysis of several aspects of DNA, RNA, and amino acid sequences. The mRNA from homo sapiens was analyzed using MacDNAsis to determine the molecular weight, number of amino acids, hydropathy, antigenicity, secondary structure, and open reading frames of the delta catalytic subunit of DNA polymerase. Amino acid sequences of the delta catalytic subunit of DNA polymerase found in five different species was also analyzed for homology. Sequence Information Figure 1.
Red triangles indicate start codons, green lines indicate stop codons.
The blue shading indicates the largest open reading frame for this molecule, spanning nucleotides 54-3377. MacDNAsis then translated the largest ORF of human delta catalytic subunit of DNA polymerase into amino acid sequence, producing a sequence of 1108 amino acids with a molecular weight of 123,628.57kDa. . MacDNAsis amino acid translation of human mRNA from the delta catalytic subunit of DNA polymerase. Using the amino acid translation, structural information about the protein could be predicted. Kyte-Doolittle Hydropathy Plot for the delta catalytic subunit of DNA polymerase.