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The Radioisotope Center has facilities for biochemical experiments using radoactive tracers and is equipped with different kinds of radiation sources needed for studies in radiation genetics. The Center has recently expanded to accomodate the large increase in experiments using radioisotopes. Molecular biology of Bacillus subtilis sporulation Sporulation of many gram-positive bacilli represents one of the simplest system of differentiation. The process starts with formation of an asymmetric forespore septum, and is followed by differential gene expression on the chromosomes separated into the two compartments. Multi-component phosphorelay transfers sporulation signal to the regulator protein of transcription, which induces the expression of the genes of RNA polymerases specific to sporulation.