This procedure is used for immunoperoxidase staining of human lymphoid tissue with monoclonal antibodies detecting cell surface antigens. The staining technique consists of two stages. First, the tissue section is incubated with the unconjugated primary monoclonal antibody. The secondary antibody, Goat Anti-Mouse IgG conjugated with horseradish peroxidase, is then applied, binding to the primary antibody. The enzyme-antibody complex is visualized by development with a substrate chromogen.
Reagents contain sodium azide.
Sodium azide is harmful if swallowed. Keep out of reach of children. Keep away from food, drink, and animal feedingstuff. Wear suitable protective clothing. If swallowed, seek medical advice immediately and show this container or label.