One kb is 1000 base pairs. Metaphase - Stage of the cell cycle in mitosis or meiosis when the chromosomes have reached their maximal condensation and individual chromosomes can be identified. Metaphase spread refers to the view of a cell's chromosomes at the metaphase stage spread on a slide. When used in conjunction with DNA or RNA, the NP-40 must be DNase and RNase free. Oncogene - A dominately acting gene that has transforming ability, i.e., causes a cell to grow in an uncontrolled manner and is responsible for tumor development.
Oncogenes play a role in the cell cycle.
Mutation, over-expression, or amplification of oncogenes in somatic cells may cause the cell to transform. A proto-oncogene is the normal equivalent of the oncogene. A mutation in p53 or a deletion of the gene is associated with tumor progression. C hain R eaction. PCR is the in vitro amplification of short DNA sequences.