The distance the pigment moves in a given time period depends on what solvent is chosen. Chlorophyll a is in the reaction center. The reaction center is a location in the membrane of the chloroplast. Electrons from water transferred to photosynthetic pigments in the chloroplast. The spectrophotometer measures the amount of light that is transmitted through a solution at a given point in time.
Light is necessary for the electrons to be boosted from the pigments.
If no light is present, no electrons will be boosted, and therefore DPIP will not be reduced. Chloroplasts in the dark, therefore, cannot reduce DPIP and so DPIP remains present in the oxidized form through which 605nm light is not transmitted. This is the result of chlorophyll in the solution. This entire site protected by copyright. All rights reserved.