The relative insolubility of this compound and its toxicity may lead to renal malfunction and stone formation. There are two protocols for this assay, one is used for red blood cells and one for cultured cells of any type. In the RBC assay the product of the reaction is bound to a filter under conditions that do not allow the binding of the substrate. In the cultured cell assay the substrate and product are separated by thin layer chromatography. After chromatography, the compounds may be visualized by ultraviolet irradiation or by autoradiography.
Quantification is done by liquid scintillation counting.
Turn around time is 3-5 days. Prenatal diagnosis can be performed on cultured amniotic fluid cells or chorionic villus cells. Prior contact with the laboratory is required before submitting any samples for prenatal diagnosis. Turn around time is dependent on cell growth but is usually 1-2 weeks. Whole blood , 3-5 ml in heparinized tube.