The embryo can be removed and aseptically grown, thus a valuable cross can be rescued. Hopefully I will add photos of this method in the fall of 2000. 31 Plantlets with small shoots emerging appears to be the best stage to treat. They are transferred to stage four media for 3-4 days, then transferred back to stage two media. Tetraploid version sometimes doubles and polys.
Pollen is also very difficult to use.
34 One way of confirming whether a plant is tetraploid is to check the pollen. 35 Middle photo is a 10 mm, 0.1 division linear reticle . With out confirming whether pollen is tetraploid, one may waste an entire hybridizing season to find out that all the pods have fell off due to use of diploid pollen on your tetraploid plants. Use of a microscope for confirmation of conversion is mandatory.