Click here to get the Biotechniques paper in PDF format. Examples of all multiplex mixes are presented below. Arrow indicates the presence of an unspecific product. Although not desirable, this product did not interfere with the use of mix E in a microdeletion screening project. Occasionally, mix C was used without the primers for loci 4 and 5.
Primers amplify nonpolymorphic loci.
The sizes of the longest and the shortest product are also indicated. These primers amplify polymorphic loci, and alleles of different sizes can be observed. The shortest alleles of locus 6 and the longest alleles of locus 7 can, sometimes, overlap, making it difficult to assign precisely their origin. The sizes of the longest and the shortest product are also indicated.