Do not forget to flame cap and bottle each time it is opened. Soil Moisture Determination 1. After autoclaving, glass pipets can be disposed of in waste glass container. Used pipet tips and other materials may be disposed of in appropriate waste containers after autoclaving. Rinse dilution bottles in sink, making sure to catch any soil waste and glass beads in fine sieve.
Rinse glass spreading bars, wash bars and dilution bottles in dishwasher.
Autoclave to reuse spreading bars. Refill dilution bottles with MPP buffer and autoclave for later use. Martin's medium plates are to be read 3 and 10 days after inoculation. Starch Casein plates are to be read 14 days after inoculation. Walker, J.D., and R.R. Colwell.1976.