Liver regeneration provides an ideal model to study gene regulation during cellular growth.
In the mouse, the level of AFP gene expression during liver regeneration is regulated by the Afr2 gene. We have mapped the Afr2 gen e to mouse chromosome 2 approximately 76 cM from the centromere. Cloned DNA from the region will be used in exon-trapping and direct selection of cDNA to identify potential genes. He mice in mRNAs isolated fromregenerating liver. My laboratory has identified the element of the AFP locus required for Afr2 regulation of AFP gene expression during liver regeneration .