The following guidelines have been prepared by the Animal Subjects Committee primarily at the request of the faculty in the Department of Biology as an example of an appropriate summary portion in an animal use protocol application for the use of rabbits in antibody production. It should be noted that this is intended only as a suggestion and not as the only acceptable summary statement. PI's are not required to use this format, but may use it to assist in preparing a summary statement.
Rabbits are a well-established species for the induction of specific antibody responses.
The injection sites must be observed at least weekly for evidence of inflammation or large granuloma formation. Small blood samples of 5 ml or less may be obtained from the lateral ear vein. At this time it is recommended that PCV and TPP be determined. Sites of inoculation will be examined daily. The Animal Care Program will be notified if evidence of severe inflammation, large granulomas, ulcers, pain or distress is found.