Translation ends at a stop codon, or non-sense codon. The genes are thus coordinately expressed, but each contains its own ribosome binding site. Grouping of several operons under the control of a common regulatory protein is a regulon. Polarity occurs when a mutation in an operon effects genes downstream in the operon. For example, a transposon insertion in an operon generally prevents expression of downstream genes.
Nonsense codons can be polar because they block translational coupling or promoter mRNA degradation.
Activators promoter RNA polymerase binding while repressors block. Figure 1 Figure 2 1. Membrane sensor histidine kinase and cytoplasmic response regulator. The sensor is phosphorylated upon activation at a histidine residue and by a phosphorelay transferred to an aspartic acid residue on the response regulator which is a transcription factor. Phosphorylation of the response regulator activates its transcriptional activation.