Evolution is an iteration between diversification and selection.
A general protocol for establishing such high affinities was developed. These mutations provide new leads for defining the biophysical principles underlying these affects. The established evolution procedures are now being applied to a number of challenging problems of fundamental biological importance. This technology is based on in vitro translation, in which both the mRNA and the protein product do not leave the ribosome. Correctly folded proteins can be selected, if the folding of the protein on the ribosome is secured.