Well Comments Minimize pressure surges Make rapid injections to reduce pressure changes during sample injection. Maintain pumps to minimize pressure swings resulting from inconsistent flow rates. Place each of these before the column and after the injector. An in-line filter will catch large particulates and a guard column will prevent strongly adsorbed materials from reaching your analytical column. Frequently flush columns with a strong solvent.
Many strong solvents are immiscible with aqueous containing mobile phases.
Remember to flush the column and HPLC system with isopropanol prior to and after the use of CH 2 Cl 2 . Use column temperatures of less than 60 C. Check column manufacturer specifications. Keep mobile phase pH between 3 and 7 for silica based columns. Add 100-200 ppm sodium azide to prevent bacterial growth. Stagnant aqueous reservoirs readily generate bacterial growth which can cause dramatic baseline disturbances and plug columns.