Restriction enzymes are enzymes that digest DNAs at specific DNA sequences. In evolutionary studies, they can be used to determine if a DNA sequence recognized by a restriction enzyme varies between isolates. To digest DNA with a given restriction enzyme, DNA is combined with the enzyme in a buffer that is appropriate for optimal enzyme performance. The ability of the enzyme to digest the DNA is assayed by gel electrophoresis.
Procedures vary with the type of DNA sample.
In order to get the best digestion activity, the correct buffer and temperature must be used. Usually, the appropriate buffer is shipped with purchase of the restriction enzyme. Buffers with the same number from different companies are not the same buffer. Select one DNA sample to test different enzymes . The DNA that you have used as a PCR control is a good bet because you should have a large quantity of this fragment.