Add 200ul wash buffer to each well, then rock 1hr. Remove wash under LOW vacuum. Add 200ul wash buffer, seal plate, then rock overnight at 55 degrees C. 17. Rinse twice with PBT . Wash 5 min at RT with AP buffer.
Embryos are ready to mount or dissect once they have settled to the bottom of the well.
RNA guard Rnase inhibitor Applied Sci.