The pKa values are necessary to calculate the pH of a solution. The ability of a buffer to resist large changes in pH is its buffering capacity. Buffers either absorb or release protons to maintain the pH within narrow limits. In the blood, dissolved carbon dioxide forms a weak acid, carbonic acid, which acts as a buffer. HCO3 - water carbon carbonic acid proton bicarbonate dioxide ion Buffers are essential in most biochemical research protocols.
Enzymatic reactions require particular pHs.
Determination of the correct pH and the choice of the most appropriate buffer requires skill and an understanding of the particular biochemical system. In some instances, a buffer, due to its chemical composition, may interfere with a reaction. The importance of choosing the correct buffer cannot be overemphasized. Use and calibration of a pH meter a. Rinse the pH electrode with distilled water using a wash bottle. Place the electrode in the pH 7 standard solution.