A new version of DNA probe localization technology on chromosomes and chromatin is suggested. In the first case only the perfect duplexes formed by DNA probes with matrice supply the polymerase extension of the probes and become anchored to matrice after irradiation, that is more suitable for localization of small probes.
Extensive washing removes most of the non-specific signals from the samples.
The second approach is more simple for bigger probes. Both technologies will be illustrated by examples. The further development of this approach might provide a possibility of localization of very short probes both on chromosomes and on interphase chromatin. Abstracts scanned from text submitted for November 1994 DOE Human Genome Program Contractor-Grantee Workshop.