The goal is to prepare liver microsomes. Thank you in advance.
Liver tends to be a soft tissue ang grinds easily.
The problem that we have encountered with the electric homogenizers for microsomal work is that they tend to generate alot of heat and may inactivate microsomes. Even the PE tends to generate some heat, and we place the grinder in an ice bath while we grind. The other problem with the electric homogenizers is that it is sometimes difficult to get all the tissue back out of them without excessive rinsing. It is easier to rinse the PE homogenizer.