Other Pages Identification of centromere sequences led to the realization that they are not conserved in evolution. Region I is not essential for CEN function, but plays an enhancing role. Region II is AT rich and binds histone-like proteins. Region III is essential.
Morphological observation suggests a single microtubule binds at each centromere.
Kinetochores are indistinct in this yeast. Each of the three CEN's has a central core sequence flanked by inverted repeats. Flanking the repeats are members of repeated sequence element families. Some are tandemly repeated. Others are interspersed with other repeats.