Due to the numerous polar compounds in culture media, chromatographic interferences may be observed with ion exchange chromatography or if an ion-paring agent is used in a mobile phase. The antibiotics are included to minimize potential bacterial contamination. Phenol red can inhibit phase II glucuronidation reactions. Due to the numerous polar compounds in culture media, chromatographic interferences may be observed with ion exchange chromatography or if an ion-paring agent is used in a reverse phase mobile phase. The antibiotics are included to minimize potential bacterial contamination.
Phenol red can inhibit phase II glucuronidation reactions.
Due to the numerous polar compounds in culture media, chromatographic interferences may be observed with ion exchange chromatography or if an ion-paring agent is used in a reverse phase mobile phase. Due to the numerous polar compounds in culture media, chromatographic interferences may be observed with ion exchange chromatography or if an ion-paring agent is used in a reverse phase mobile phase. Due to the numerous polar compounds in culture media, chromatographic interferences may be observed with ion exchange chromatography or if an ion-paring agent is used in a reverse phase mobile phase. Warm a physiological buffer to 37 C for about 1 hour prior to using. If this rinse buffer is not at 37 C, it will be ineffective in removing the solid matrix that covers the cells.