If you ask intelligent questions, are enthusiastic and a good listener, you will leave a good impression. Remember. . . Be alert and confident. Relax and enjoy the conversation and the opportunity to see what the company is like. If you are nervous, it will make your interviewer nervous, too, and that will not leave a good impression.
People often hire based on feelings and impressions.
Each interview is a chance to learn how to present yourself in the best light. Each is a chance to be sure this is a place you will feel safe working and a place your skills will be appreciated.