T his approach allows the identification of cellular sites of mutant gene action and the tracing of cell lineage. The neurohistological techniques we employ to study this problem are in situ hybridization, immunocytochemistry, and image analysis. We also use embryo manipulation, molecular techniques including use of restriction fragment length polymorphisms, and extreme thoughtfulness in our studies of brain development. The structure of clonal and polyclonal arrays in chimeric mouse retina. Genetic mutations affecting murine cerebellar structure and function.
Hybridization analyses of nervous system gene expression.
Mouse chimeras in the study of genetic and structural determinants of behavior. Trends in Neurosci. Developmental regulation of various annexins in the embryonic palate of the mouse, submitted. Genetic and environmental control of variation in retinal ganglion cell number in mice. Arbitrary primer PCR of dog DNA with estimates of average heterozygosity.