The patient should not urinate into the collection bottle. If one of the samples is accidentally lost or removed within the collection period, the entire collection must be restarted. At the end of the collection period the bladder is emptied and the specimen is added to the collection. Note the ending time on the request form. If removing an aliquot of urine, the total volume for the timed collection must be noted on the request form.
Females Remove appropriate garments for urination.
Wash hands thoroughly. With one hand, separate the urinary opening and hold open until the process is complete. Using a saturated soapy sponge, wash from front to back three times using a different soap and pad each time. Wash with warm sterile water to remove soap residue. The patient should void a few milliliters into the toilet and then collect urine in a sterile container.