Voltages higher than 150 volts are not recommended because of heat generation in the v-shaped channels which causes bubble formation. Recovering nucleic acids. Turn off power supply. Open the two upper clamps first, then slowly open the drain hose clamp. When the buffer level has dropped below the slices in the well you can remove the salt cushion.
Many procedures demand nucleic acids free of other contaminating nucleic acids.
It is recommended that the v-shaped channels be electrophoretically cleaned immediately after using the apparatus. Simply remove the gel slices, refill the channels and wells with buffer and run for a while. Discard buffer, rinse with distilled water and store dry.