In addition, nearly all euglenoids can swim using one or more flagella for locomotion. Chelex 100 resin by Bio-Rad. The amplified regions of SSU rDNA were purified by gel electrophoresis. The rDNA band was cut out of the gel, followed by a Qiaex Gel Extraction procedure as described by Qiagen. In separate experiments, the purified template was also sequenced by using the ABI automated sequencer at Robert Wood Johnson Medical School per their protocol.
Parsimony analysis attempts to explain the given data using the fewest number of steps possible.
The data set was scored with a binary code, depending on the alignment of the primary structure of all 10 organisms. A majority rule analysis was performed on the data set as well, using 500 replicates, to assess the robustness of the tree. The taxon E. anabena has been treated differently by various authors. However, my observations of E. anabaena var . One visible flagellum, located at the anterior end of the cell, is approximately one-half of the body length.