One option is to set up 5 different stations, one for each of the activities and have the students work in a jigsaw arrangement. Each group of 5 students will send one member to one of each of the stations. The students can propose their own hypotheses and design their own experiments. For this lab, you will be using homogenates. Using an homogenate allows some control over volume of enzyme used in each experiment.
Homogenates are easy to prepare.
They are biological catalysts. Label 4 test tubes A, B, C, and D. Put 3 mL liver homogenate in each tube. Put 3 mL of the following solutions in the test tubes as noted. How do you account for the differences in the reactions with liver homogenate and the reactions with pure catalase. Record as reaction A on Data Chart 2.