Now you can go back to Table 6.3 and calculate how many micrograms of protein you added to each beta-galactosidase assay tube. Plot A420 vs. micrograms of protein in the cell-free extract. Convert A420 to micromoles of ONP produced, using the beta-galactosidase calculations below. N.J. Rosebrough, A.L. Farr and R.J. Randall. M.D. Provezano, E. K. Fujimoto, N. M. Goeke, B. J. Olson and D. C. Klenk.
Protein assay using bicinchoninic acid.
Bradford, M.M. 1976. A refined and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.