You may cut these strips with a paper cutter and then place them in 18 x 150 or 16 x 150 culture tubes and cover with a cap or alumin foil before autoclaving. Huff St., Palmyra IN 47164 Please send an e-mail containing your ideas. For safety, have someone familiar with pressure cookers help you with this half of Expt 2. We still have a problem. The ingredients and the glass or jars will already contain bacteria.
Lets try the most important step in Pure Culture Methods.
That is autoclaving the medium. Place on fire, and when steam is coming out nicely, turn flame down and continue cooking for 15 minutes at 15 pounds pressure. Fiveteen pounds of steam pressure for 15 minutes will kill all bacteria, viruses, spores, and other living things. Let it cool, it is best to let it cool to cold gelled medium before you remove the lid. The cooker is a good sterile place for your sterile glasses of medium.