If the mites aren't detected immediately, they spread. The treatment may have to be repeated several times to kill them all. However, I would not soak them in the solution more than twice. The hemipenes will evert easily, but this should only be done by experienced individuals, as damage from excess pressure is possible. In this method, a metal probe is slipped through the vent to check for the presence of a hemipenes, the male copulatory organ.
Every male has two hemipenes in the base of the tail.
Females will probe from 2 to 4 subcaudals. Males will probe to 7 to 8 while immature, the adults will probe to 10 to 12 scales. When looked at from the side, male tails are generally half as thick at the middle as at the base. Female tails are usually half as thick a quarter of the tail's length from the base. While learning, look at adult snakes of known sex before trying it on juveniles and adults of unknown sex.