This experiment is to practice the measure of concentration using a spectrophotometer. This requires a mastery of the operation of the spectrophotometer. To perform the experiment, the experimenter will perform a serial dilution. In this process, a solution of know concentration is repeatedly diluted following a pattern to construct a set of standard dilutions. This flask has been carefully calibrated to contain the designated volume.
Molarity is measured in moles per liter.
A sample of a pure substance is carefully massed. This is done on a suitable balance for the amount to be measured. If an amount less than one gram is needed, it is necessary to use an analytic balance to provide more than 2 significant figures for the concentration. It is not necessary to dry the flask. The flask is partially filled with the solvent.