Within the ten steps of the Krebs Cycle, it requires two turns to metabolize each glycogen molecule. The unstable bond of acetyl coA breaks and its two products bond to the four carbon oxaloacetic acid to form a six carbon citric acid. Now, carbon dioxide leaves the Isocitric acid and the six carbon molecule becomes a five carbon one. This phosphate group is then transferred to GDP to form GTP and succinic acid. The succinic acid is oxidized to fumaric acid and FAD is reduced to FADH2.
Oxaloacetic acid is regenerated to begin the cycle again.
If no ATP is present, the body will be unable to metabolize the fat while maintaining muscle. Pyruvate has the ability to maintain plentiful amounts of ATP so the metabolism will be at the highest rate possible. Pyruvate is found in the natural diet, but only in small amounts containing less than 25 mg in each serving. This means that the mixture of pyruvate with the DHA increased the time period to exhaust the arms and legs by 23 and 13 minutes, respectively. Recently the researchers have said that the pyruvate alone works even more effectively.