All Centricon retentates possessed inhibitory activity and haemagglutination, while stimulatory activity with out haemagglutination was observed with the Centricons filtrates. This stimulatory activity apparently has a molecular weight below 10,000. SDS-PAGE analysis was performed on the protein extracts and heavy banding above the 50,000 M.W. size range was observed corresponding to inhibitory activity in the Centricon fractions. Haemagglutination was also observed with these extracts. Change in Nematode Population in Cultures with KRS-Fractions.
Negative numbers indicate a stimulatory or growth promoting effect of the fraction.
Change in Nematode Population in Cultures with Individual Fractions. Negative numbers indicate a stimulatory or growth promoting effect of the fraction. Protein concentrations in micrograms. Heated extracts were taken to 100 C for 5 minutes. Change in Nematode Population in Cultures Containing Active Fractions After Ultra-filtration.