Calculation of transformation efficiency. Insect cell tissue culture, passing and counting cells. Homologous recombination in insect cells. Molecular modeling of proteins. Amplify by infecting new cells.
Enzyme activity assays.
Purification and concentration. There will be one lab final. There will be problem sets and quizzes given in lab stressing calculations commonly used in molecular biology. The paper will be in the format of the Journal of Biological Chemistry. Complete with Abstract, Introduction, Materials and Methods, Results, Discussion.