Problems result from protein denaturation or starch gelatinization.
Our tests using tetrazolium dye have been successful in visually showing heat damage. Dehydrogenase enzymes involved in respiration react with the tetrazolium, resulting in an insoluble, red formazan color in living cells. Non-living cells retain their natural color. The unheated kernels had a bright red stain. U.S. Department of Agriculture Corn quality factors are important for corn wet milling and dry milling.