A new class of profluorescent protease substrates that utilize intramolecular fluorophore dimers for fluorescence and absorption measurements and a molecular system for drug delivery to intracellular targets. OncoImmunin, Inc. recently designed and synthesized a new class of profluorescent protease substrates for enzymatic activity determination in serum as well as whole cells.
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To ensure specificity, all substrates contain amino acid sequences from both sides of cleavage sites, i.e. from P n through P n '. Our profluorescent protease substrate design allows determination of intracellular enzymatic activity using a fluorescence microscope. Determination of activity in buffer solution as well as in human serum can be accomplished using a standard fluorometer, or for large sample throughput, a fluorescence microplate reader with a standard filter set. Note- Absorption measurements are also possible. Inc. All rights reserved.