Wells containing samples are defined as U1, U2, U3 and so on. The standards should preferably be positioned in the middle of the microplate and the samples symmetrically on opposite sides of the series. This will prevent the error caused by the time difference between the addition of LAL reagent to standards and samples. 50 U1 G U6 C1 C1 U6 H Other plate layouts can also be used. If only a few strips of the plate are used the standards and samples can be added in order from well A1 to H1 continuing with well A2.
Many substances can affect the results of the endotoxin test.
If interference is seen, this can usually be overcome by diluting the sample. By using the Genesis worklist option it is possible to create worklists. Click the Supervisor mode box and in the Instrument box select the iEMS MF Reader. In the Run option select the specific test protocol to be run. A specific Plate Layout and Worklist can also be chosen.