At the top of the page, select the number of discriminant functions to use, namely 5 or 1. Using 5 discriminant functions, you can get better prediction results, especially for 5 and 7 transmembrane proteins. Enter the sequences in the next window.
Fasta format is available.
Or you can also specify a file of fasta sequences on your local disk. If you want to have the prediction of position of transmembrane segments afterwards, TSEG is executed by clicking the ID of an identified transmembrane protein. The discriminant function is used which was constructed to discriminate the most hydrophobic 17 residue-long segments in sequences from 2 groups of the training set, membrane proteins and globular proteins. The training set of membrane proteins consists of 3250 sequences of membrane proteins. Then similar subgroups are merged to form 5 groups.