Various ontogenetic changes in the substrate proteins for PKA and CK2 were also noted. While, the number of PKA substrates progressively increased during development, reaching the highest level at adulthood, the number of CK2 substrates did not change. Developmental changes in the intensity of endogenous substrate proteins phosphorylation were consistent to the level of PKA and CK2 activity. The investigations were performed on 5 domestic dogs aged 3 to 7 years, of both sexes, on carcasses and live animals of large rasses. On carcasses, the providing nerves of arterial muscle rings of limbs were operated and in live animals the rings were denervated in cranial tibial and median arteries.
Obtained results are very interesting.
In chilly and cold conditions, denervated limbs, observed during 3 years, was permanently characterized by higher up to 6 C temperature of digits. However, equal temperature of digits of denervated and not denervated limbs were observed at high room temperatures. Obtained results can have practical importance for clinics specializing in medication of limb artery diseases, and after proper adaptation, also for people at the stage of vasocontraction, preventing pathological changes in hands and feet. The standardised sap and extract from Echinacea purpurea L. herbs at lower concentrations appeared a potent stimulatory action on human white-blood cells in vitro . These may suggest a potent immunostimulating activity of tested solutions in humans, in vivo.