Each primer is defined with a name and its position, with each definition occupying one line. Recommended primer pairs . Each primer pair is specified with a round number followed by the left primer name and then the right primer name. Possibly overlapping view . This shows the possibly overlapping view of regions which follows logically from all given results.
Nothing is known in the first round.
Both of the displays are in plain text format. The user should save them in their local system for future use by using the file saving utility of the web browser. However, some software programs on Mac or PC don't handle newlines from the web browser correctly, although they can handle plain text in general. These software programs can still be used for saving, though, by first creating an empty file from within the software, doing copy and paste of the entire plain text display to the empty file, and then saving the file. Second Round Suppose that the user performs all recommended PCR experiments, the next step is to modify the saved new round file from last round using a plain text editor in the local system.