This softens the block face just enough to help prevent chatter or formation of microcracks. Most procedures call for mounting your sections on slides before staining. Mounting thinner sections to slides before staining also helps to prevent folds from developing in the sections. Haupt's Solution Haupt's Solution 500 ml of distilled water. Heat to 30 C. 5 g of gelatin 10 g of phenol crystals 75 ml of glycerine Stir until fully dissolved and filter.
Procedure Use a positively charged clean slide.
Place a thin film of Haupt's Solution on the slide. Place the section on the alcohol, trying to flatten it out as much as possible with tweezers, applicator stick, or a sable brush. Cover the section with a piece of polyethylene film. Add a piece of filter paper. Roll excess fluid from the stack with a wallpaper seam roller.