Viscosity Transfer 2 g of the sample into a 400-ml beaker and moisten thoroughly with about 4 ml of isopropanol. An opalescent, viscous solution is formed. Transfer 100 ml of this solution into another 400-ml beaker, heat the mixture in a boiling water bath for about 10 min and cool to room temperature. 1 - 5 l. Use galactose and mannose as reference standards. These constituents should be present.
Guar gum shows close groups of round to pear formed cells, their contents being yellow to brown.
Borate Not detectable Dissolve 1 g of the sample in 100 ml of water. The solution should remain fluid and not form a gel on standing. Mix 10 ml of dilute hydrochloric acid with the solution, and apply one drop of the resulting mixture to turmeric paper. A brownish red colour, which upon drying becomes intensified and changes to greenish black when moistened with ammonia TS, should not be formed. The percent of nitrogen in the sample multiplied by 6.25 gives the percent of protein in the sample.