Specifically we are 1. We do this through a genetic analysis of dominantly acting Notch constructs which affect the assignation rather than the suppression of cell fates, and by looking for molecular interactions of the intracellular domain in two hybrid interactions systems. Map of Notch with putative functional domains derived from the genetic and developmental analysis of various mutants. For details see references below. The possibility that Dishevelled binds to the C terminal region of the intracellular domain has been suggested by the experiments of Axelrod et al.
Notch is required for wingless signalling in the epidermis of Drosophila .
A functional analysis of Notch mutations in Drosophila . Interactions between Wingless and Notch during the assignation of cell fates in Drosophila. An activity of Notch regulates JNK signalling and affects dorsal closure in Drosophila. A repressive activity of Notch in the embryo that is antagonized by Wingless. Wingless and Notch signalling play important roles not only in the assignation of cell fates but also in the generation and patterning of large scale structures like the wings and the legs of the fly.