Departure from these guidelines requires adequate justification in the proposal. Consider the use of Incomplete Freund's Adjuvant, or another adjuvant 1 . FCA should be used only for the first antigen dose. The use of two or more doses of the complete adjuvant is rarely warranted and must be adequately justified. Injections containing FCA should be subcutaneous or intraperitoneal.
Intradermal injections can cause skin necrosis and sloughing.
Intramuscular injections may lead to temporary or permanent lameness. Intravenous injections have been known to produce pulmonary lipid embolism. Foot pad injections are not recommended. Injection of the hind limb foot pads in mice, or intradermal injections in rabbits, may be approved if injections at other sites are shown not to produce significant antibody titers to weak antigens. Foot pad injections must be carried out under general anesthesia.