If there were cells that express both CD44 and CD62L, they would appear in the upper right quadrant. The three-color method is extremely useful for quantifying the different subsets of immune cells and may also be applied in cell biology research when simultaneous analysis of three different cellular parameters is desired. Four populations can be observed and quantitated in the histogram below. This information may lead to a new method for predicting health in swine. Sytox is a green fluorescent dye which penetrates membrane compromised gram negative and positive bacteria.
Live cells exclude the dye.
Using the BacLight kit, the live bacteria stain with the green fluorescent cell permeant dye, while the dead bacteria are labeled with the red membrane impermeant dye. In both cases, total cell counts can be performed with the addition of a known concentration of fluorescent beads prior to analysis. Using a Dimeric Cyanine Nucleic Acid Stain Bruno Becker1, Jeffrey Clapper2, Kristi R. Harkins2, and James Olson1 1. YOYO-1 fluoresces brightly only when bound to nucleic acids. Cells are incubated with YOYO-1, and fluorescence is measured before and after the addition of detergent, which allows the dye to enter the cells.