Function Protein proteolytic enzyme or reagent cleavage digest Description digest finds the positions where a specified proteolytic enzyme or reagent might cut a peptide sequence. This programs allows you to input a protein sequence and to specify one proteolytic agent from a list. It will then output a file containing the positions where the agent cuts, together with the peptides produced. If you wish to emulate a partial digestion, then using the -overlap qualifier will display the results from a digest in which all cut sites are used and in which one site at a time is not cut. Here is a sample session with digest.
Overlaps are therefore fragments with exactly one potential cut site within it.
As for overlap but fragments containing more than one potential cut site are included. Overlaps are therefore fragments with exactly one potential cut site within it. No No Input file format Any protein sequence. The first non-blank line is the title containing the name of the program and the name o fthe input sequence with the range considered and the calculated molecular weight of the protein. The next non-blank line gives the name of the reagent used to digest the protein and the number of fragments reported.