With the forceps place a sterile paper towel on the workspace. With the forceps place a runner tip on the towel. Place the forceps in the other hand to hold the tip while the first hand uses the knife or scalpel to cut off 1 cm of the stem. Grab a sterile test tube of medium with the other hand, hold it by the base. With the little finger of the hand holding the forceps, remove the cap and cradle it there while you use the forceps to firmly lay the bud on the medium.
Room temperature is o.k.
8 hours darkness is standard. Check daily for contaminants. If any are found, sterilize tube and contents before discarding the contents. Transfer the explant every two weeks or so until it is actively growing. In one to two months you should be able to divide the culture into two pieces, each of which is about 0.5 cm in diameter.