Most RLUs were left on roads and trails that were on the edge of the territories. Some were left in the brush and on frozen waterways. Most of the RLUs were deposited on objects. This practice allows the mark to be better distributed. It also allows the urine to act as a visual cue.
Positive feedback seems to be the primary stimulus in causing a wolf to remark an area.
A wolf was more likely to renew his own recent marks than those left previously by itself or another wolf. They studied the location of marks as well as the body position used when leaving the marks. Lone wolves did not mark with RLUs. They also did not leave marks of any kind near kills or on top of other marks. They marked less on roads and trails than did resident packs.