However, under our conditions we were not yet able to detect the formation of additional DSBs due to enzymatic cleavage of oxidative lesions by the Fpg protein on such fragments. In the following, we want to test higher doses of gamma-irradiation and higher concentrations of Fpg protein. We observed a dose-effect relationship for the induction of these fragments of defined sizes in repair competent Chinese hamster ovary cells. The more the radiation dose increased, the more the fragment size decreased and increased the amount of fragments with 3'-OH ends. The different types of the DNA fragments induced in different conditions of irradiation will be characterized using different chemical and biochemical markers to get a better understanding of the implication of DNA repair processes in the observed dose rate effects.
Relation entre radiochimie et radiobiologie.
Acad mie des Sciences. Rapports sur la science et la technologie n 4, Juillet 2000, Ed. Acad mie des Sciences. Rapports sur la science et la technologie n 4, Juillet 2000, Ed.