Using data for 3 ejaculates from each males in a group, the ProgenyPlus personnel classify each male evaluated as a DUD, intermediate, or good. These classifications are returned to the breeder or producer, and serve as the basis to cull DUD males. Such culling will raise fertility of the herd or flock. The SBA is based on that reported by Barbato et al. This is possible because most sperm from certain males, and some sperm from many males, have insufficient amounts of a naturally-occurring protein on their outer membrane to maximize the probability of binding to the egg investment.
Peptide is produced in the laboratory, and is of known potency.
With poultry, optimal concentrations of the peptide depend on the line of rooster or tom, and possibly the female line, and should be determined for each situation. Such an onsite application study also helps to familiarize personnel with use on a small scale, before extensive use of the product.