In this communication, we also describe almost complete primary structure of A. acetabulum V type ATPase, B subunit and a partial cDNA clone with extensive divergence in its codon usage and its 3'-untranslated region.
Experimental procedures are almost the same as described in our preceding report.
The clones pVB1 to 3 were amplified with Taq polymerase, and pVB4 with Pfu polymerase. From the position 1 to 6 the EcoRI restriction site was found that was used for construction of cDNA library. Northern analysis was also performed as for two putative cDNA clones coding for V-B subunits. About 2 kb RNAs were detected for pVBL and pVB5 3'-untranslated regions specific probes with similar intensities. Localization of these respective two cDNA clones should be clarified by approaches with expression studies.