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Prism has no common sense.
When it finds the best-fit of an equation to your data, it doesn't know which values are reasonable. Prism might report that the best-fit value of a rate constant or a plateau is a negative number. Prism doesn't know that negative numbers are impossible. Setting a parameter to a constant value The most severe constraint is to set a parameter to a constant value. For example, you can set a plateau variable to zero if you have already subtracted out any baseline or nonspecific data.