The use of the oxazole yellow homodimer YOYO-1 to measure delivery of plasmid expression vector. The fluorescent DNA intercalator YOYO-1 has strong binding to DNA and high quantum yield, and thus, may be used as an intracellular marker for plasmid expression vectors transfected into tumor cells in vitro. The stability of YOYO-1 binding within the lipoplex was first examined. Analysis using DNA-mediated Gene Transfer. Cloning and Sequence Analysis of the Human Gene Encoding Steroidogenic Factor 1.
Endothelial cells are important targets for a variety of treatment strategies.
To reach other targets, for instance tumours, drugs must cross the endothelial barrier. Uptake of blood borne agents into endothelial cells occurs by receptor-mediated and fluid phase mechanisms. We are studying the endocytosis and transcytosis of liposomes and liposomal encapsulated agents to endothelial or sub-endothelial targets. Altered endothelial surface proteins have been noted at tumour sites or atherosclerotic legions may facilitate liposomal formulations which direct agents with a higher degree of specificity to disease sites over normal sites. We are attempting to generate endothelial-targeted liposomes.