For light microscopy, an immunohistochemical ABC technique was used to label vimentin. Studying the results from light and immunofluorescent microscopy, along with correlative work at the electron microscopic level, we have observed not only extensive bundles of vimentin filaments but also prominent vertices which serve as connection points for many of the filament bundles. Scanning electron microscopy was used to better visualize these vertices and verify the IMF results. Possible functions of these vertices and arrangements will be presented. Marvita D.McGuire, Stephanie Wright, Robert S. Conrad, Department of Biochemistry and Microbiology, Oklahoma State University,College of Osteopathic Medicine.
Manifestations are diverse, includmg endocarditis and septicemia.
Vancomycin, a cell wall inhibitor, is the most effective antibiotic available for this organism. The mechanism of resistance to vancomycin in MRSA is not known. Transmission electron microscopy revealed thickened cell walls, multiple septa, and irregular cell borders as compared to the MRSA grown in the absence of vancomycin. Scanning electron microscopy also provided evidence of changes in the morphology. These changes may provide evidence that increased peptidoglycan synthesis and transport alterations may play a role in the mechanism of resistance to vancomycin.