The best questions disguise what you're looking for. The applicant can't figure out what you want, so they answer honestly. But it isn't. It gives the interviewee a chance to hang themselves by being negative. Look for negativity about anything.
Negative people tend to be negative in many different situations.
That can come back to haunt you. Chances are, you're looking for positive, upbeat, dependable, honest, competitive employees who want to be superstars themselves, but who have a team attitude. That's hard to find, but these questions smoke out the small differences between people. Tell me about a time when you had a disagreement with a boss. We're three days behind schedule on a major project.