Any Escherichia coli with a cI function. Start of the nucleotide sequence in the middle of the non-unique EcoRI site between the PL and the T7 promoter. Ligation to other sites will result in the production of a fusion protein. BamHI, BstXI, HindIII, MluI and SacI cannot be used for expression, since the HindIII site contains a termination codon in phase with the ATG codon. U.S. Patent No.
Punctuation indicates reading frame.