Embryos lacking loco function have a normal overall morphology, but fail to hatch.
Ultrastructural analysis of homozygous loco null mutant embryos reveals a severe glial cell differentiation defect. Different RGS proteins vary in their specificities for the Galphai and Galphao subunits. Loco specifically interacts with the Drosophila Galphai-subunit. The strongest loco allele is represented by the embryonic lethal mutation loco delta13 . No abnormal CNS axon pattern phenotype is detected using the mAb BP102, which labels the overall axon pattern.