Transformation systems will also allow the genetic manipulation and modification of pest and vector species. This will result in the development and improvement of marked genetic sexing strains for use in Sterile Insect Release programs. It should also be possible to develop strains of vector species that can no longer transmit a particular virus or parasite, thus providing an alternative method of control. Research in my lab is aimed at improving the transformation system for the yellow fever mosquito, Aedes aegypti . Specific projects involve the use of enhancer elements to increase transgene expression levels and the use of boundary elements to insulate the transgenes from position effects.
Efforts are ongoing to develop transformation systems for other important insect species.
These studies involve the testing of transposable element activity, promoter usage and marker gene suitability. A number of techniques are available that allow a directed molecular search for novel, secreted and membrane-bound proteins in target organs such as the midgut and salivary glands. Discovery of such proteins will provide useful targets in the development of transgenic plants and insects, and also new options for vaccine and drug development to combat agricultural pests and vector species. An eye color gene for the detection of transgenic non-drosophilid insects. Insect Biochem. and Molec.