In addition, should you be interested in Phase II glucuronidation reactions, we recommend using a phenol red free medium. LeCluyse, E. L., et al. Sidhu, J. S., et al. This medium is a typical hepatocyte full nutrient media. Due to the numerous polar compounds in culture media, chromatographic interferences may be observed with ion exchange chromatography or if an ion-paring agent is used in a mobile phase.
Phenol red can inhibit phase II glucuronidation reactions.
Due to the numerous polar compounds in culture media, chromatographic interferences may be observed with ion exchange chromatography or if an ion-paring agent is used in a mobile phase. The antibiotics are included to minimize potential bacterial contamination. Phenol red can inhibit phase II glucuronidation reactions. Due to the numerous polar compounds in culture media, chromatographic interferences may be observed with ion exchange chromatography or if an ion-paring agent is used in a reverse phase mobile phase. The antibiotics are included to minimize potential bacterial contamination.