This web page was produced as an assignment for an undergraduate course at Davidson College. Methods A yeast two-hybrid system for discerning differential interactions using multiple baits Introduction Proteins are highly involved in cellular function.
Many proteins act by interacting with other proteins.
Molecular biologists are begining to sequence many different proteins, but their function is often times unknown. The function of these newly discovered proteins can be investigated by studying the relationship that they share with other known proteins. Sequencing can then be made by screening a cDNA library. The original methods have recently been improved upon by the addition of multiple reporter constructs, and by using a reverse system to test for potential interference of protein function. This is a model of transcriptional activation showing how the Bait protein is linked with the transcription activating region so that as the bait protein and the prey protein enter into close proximity, transcriptional activity can take place.