Remove supernatant by careful aspiration. Analyze in a flow cytometer according to manufacturer's instructions. Proper color compensation is important for unbiased data interpretation. Cell samples stained with the corresponding single reagents of the pair may be used as controls for adjusting compensation. It is advisable to run the appropriate negative controls.
Negative controls establish background fluorescence and non-specific binding of the antibodies.
The ideal negative control reagent is a combination of a FITC, PE, and Quantum Red conjugated mouse monoclonal or myeloma proteins which have no reactivity with human cells. The degree of autofluores cence or negative control reagent fluorescence will vary with the type of cells under study and the sensitivity of the instru ment used. Chorvath, B., et al. Dalchau, R., et al., Eur. Additional terms and conditions may apply.