The chromatin structures of genes and other DNA sequences are also examined in animal tissues and in transgenic mice. Also, we have written computer programs that detect newly discovered features of the DNA sequence that appear to direct nucleosome positioning and array formation. The predictive power of our algorithm is being tested, and the algorithm is being refined. Computational methods are also being developed to perform comparative genetics. Important biological questions can be addressed by identifying and comparing regulatory DNA sequences in othologous mouse and human genes and in different individuals.
Genes involved in development are of particular interest.
Additionally, we have developed an ultra-thin disposable miniaturized gel for DNA sequencing. They run in a few minutes, use very small amounts of reagents, and are loaded in a novel way, suitable for automation. We are further improving the performance of this system and are developing new methods for non-radioactive and automated detection. Resolution comparable to that of conventional gels may be possible. Simple miniaturized gel system for DNA analysis, Nucleic Acids Res.