Second, activated CS was isolated on a Sephadex G-25 column equilibrated with the same buffer. Cu,Zn-SOD from rat liver is an oxidoreductase composed of two identical 16 kD subunits. Catalytic activity is attributable to the dimeric SOD molecule but not to a single subunit. According to native electrophoresis, SOD easily self associates. Arrows correspond to the positions of marker proteins.
Electrostatic interactions initiate the formation of such a complex.
The impact of these interactions on the complex formation between SOD and CS was evaluated. The data of Table 1 indicate that electrostatic interactions play a significant role in interaction of the SOD subunits with each other. Increasing the ionic strength of the solution significantly weakens the interactions. This is the next stage of the study. N. Y. Acad.