The rapid increase in the number of recombinant proteins and monoclonal antibodies used therapeutically has created a demand for methods that accelerate and simplify generation of high producing, stable cell lines. In this system, a derivatized gel matrix surrounding each cell functions analogously to an antibody-coated microtiter well, capturing the protein of interest within the immediate surroundings of the cell. Using FACS , the fluorescence intensity of each GMD can be quantified and high secretors isolated and recovered based on the level of secretion. Any secreted molecule for which there is an appropriate antibody pair can be captured and quantified.
Productivity is confirmed by analyzing the culture supernatant using a standard immunoassay.
In addition, the secretion assay can be used ro rapidly isolate the small population of secretor cells after trancfection and to access the productivity of cells during fermentation. This high throughput screening method improves productivity and shortens biotechnology product development timelines. Add biotinylated capture antibody. Secretion incubation, typically 15 minutes. Add fluorescently labeled detection antibody.