The OxyBlot TM Kit is applicable to purified or crude protein prepara-tions. Samples may be transferred to either nitrocellulose or PVDF membrane. Positive control provided in kit allows interblot comparisons. Fast 1 minute exposure by chemiluminescent detection. Oxygen-derived free radicals have been implicated in various physiological and pathological processes such as aging, apoptosis, cancer, neurodegenerative diseases, chronic inflammatory diseases, pulmonary diseases, and cardiovascular diseases 1 .
Proteins are one of the major targets of oxygen-free radicals and other reactive species.
The Oncor OxyBlotTM Kit provides a new, sensitive method to detect and quantitate oxidized proteins immobilized on nitrocellulose membrane 4 . Pognonec, P., et al. Levine, R.L., et al. Carbonyl assays for determination of oxidatively modified proteins in Methods Enzymol. The proteins were transferred onto nitrocellulose membrane and DNPH-proteins were detected using the OxyBlot TM Oxidized Protein Detection Kit.