4.2.2.10 Purified pectinase is a multi-component preparation highly effective in depolymerizing plant pectins with varying degrees of esterification. The product contains substantial hemicellulase, cellulase, pectinesterase and xylanase activities which together with pectin lyase and polygalacturonase work synergistically to digest plant cell wall tissues. The enzyme is assayed using a method which quantitatively measures the release of D-galacturonic acid from polygalacturonic acid by determination of reducing sugars using a neocuprine hydrochloride color reagent assay. When used with Worthington purified cellulase, purified pectinase has been found to be highly successful for generating good yields of viable protoplast in several plant systems, e.g., corn, soybean, red beet, sunflower, tomato and citrus. Protect from moisture.
Material is very hygroscopic and can get tacky and hard to weigh if exposed to moisture.
J Bact . Pectic Enzymes, Adv. PASE A chromatographically purified preparation also containing hemicellulase, cellulase, pectinesterase and xylanase activities. Suitable for plant protoplast isolation applications. Supplied as a dialyzed, lyophilized powder.