The Materials and Methods sections of research papers Friends and colleagues Use multiple information sources to get a complete picture. Single sources frequently omit important considerations. However, multiple sources frequently propagate errors, so rely also on your own experience. Working in your own laboratory with these techniques will provide you with command of the details of the techniques relevant to your own needs and will aid you in appreciating the principles and details of these techniques as they are used by others. All of these techniques are evolving.
None is optimal yet.
Try experimenting to improve the techniques you need for your own work. Some important tips In all biochemical reactions, alterations in pH, temperature and ionic composition can lead to dramatic effects. Be careful in adjusting the pH of your buffers. If a reaction does not work as intended, check the pH and ionic composition. Similarly, be careful to control temperatures properly.