Figure 2 shows that DNA binding protein has one segment that crosses the 1.8 threshold. This suggests that this segment of the protein may have a transmembrane domain. However, this is the only peak in the plot that is hydrophobic enough to be a transmembrane domain, indicating that DNA-binding protein is most likelly not an integral membrane protein. Hopp and Woods antigenicity plot. This plot shows the hydrophyllicity of this segment of protein.
Posative values indicate hydrophyllic areas,which are also antigenic.
The threshold line is set at 1.8, and the majority of the protein is located between this value and 0.00. The average hydrophyllicity of the area is given as 0.048. Amino acids 1-1148 are included in the range of the plot. This model, developed by Hopp and Woods, shows the hydrophyllicity of the protein segment analyzed. Figure 3 demonstrates that DNA binding protein is more hydrophyllic than hydrophobic, with an average value of 0.48.