I Expressed Sequence Map of Chromosome 16 Michael R. Altherr, Darrell Ricke. Larry Deaven and Robert K. Moyzis.
Individual chromosomes provide the skeletal framework on which genetic data are organized.
Assuming that there are 100,000 human genes, approximately 3,000 should be encoded by chromosome 16. To this end, we have chosen the strategy of exon amplification to identify expressed sequences on chromosome 16. The strategy employed 96-well plate pools of DNA from the flow sorted and arrayed chromosome 16 cosmid library as the substrate for exon trapping. We have generated and archived more than 3,024 exon clones from 126 plates in the chromosome 16 library. These sequences were analyzed using available databases and are being mapped to specific locations on chromosome 16 using a grided array of previously fingerprinted cosmids that are integrated into a high resolution physical map.