Turn the Michaelis-Menten equation upside down. Make a L-B plot from the data in the glucose problem. When the specific activity is a maximum the protein is believed to be pure enzyme. Inhibitors may be reversible or irreversible. E.I Competitive inhibition can be overcome by increasing the concentration of substrate.
Noncompetitive inhibitor Decreases the turnover number of the enzyme.
We can picture the action as a binding of I to a second site which is independent of the S-site. This usually just removes enzyme from solution by turning it into inactive protein. K m since the enzyme that is acting is the same enzyme, just decreased in concentration. Lineweaver-Burk plots are used to distinguish the two types of inhibition. Penicillin is an irreversible inhibitor of an enzyme involved in the building of bacterial cell walls.