Procedures are easily scaleable and result in high yields of target mRNA, and the beads may be regenerated to ensure low cost isolations. Efficient, rapid removal of BioMag particles from a suspension is achieved through the application of an external magnetic field. All lysis, wash and stabilization reagents are included in the kit. BioMag SelectaPure mRNA isolation kit purifies mRNA from total RNA in 15 minutes or from tissue and cell samples in 45 minutes. No Contamination Nuclease-free, highly specific BioMag particles reduce ribosomal contamination associated with columns and centrifugation.
Rapid isolation helps keep mRNA intact.
The mRNA is suitable for cDNA synthesis, PCR, in vitro translation and many other downstream applications. 20 particles may be regenerated up to 6 times using an overnight protocol with commonly available laboratory reagents. No columns are required for this method of mRNA isolation. 25 magnetic particles and magnetic particle separator. 50-100 mg tissue.