Within limits, increasing primer concentration may improve the outcome of the PCR reaction, and should be considered as a way to optimize PCR reactions. This is exemplified in Fig. 14 below, in which increasing primer and template DNA concentrations, both improve the results. PCR with various concentrations of genomic DNA template and various concentrations of a degenerate primer. At each primer concentration, reaction improves with increasing amount of template.
Reaction also improves with increasing amount of primer.