This method consists of substituting Osmium Tetroxide with formaldehyde, along with the addition of glacial acetic acid to the chromating fluid. Golgi Method without Osmium Tetroxide for the Study of the Central Nervous System. Opinions differ regarding the side of grids most suitable for permanent adhesion. The controversy is easily solved by examination of the surfaces involved. Grids are manufactured with a dull or rough side, and a shiny or smooth side.
Epoxy sections exhibit a bumpy surface when viewed in the boat.
Scanning electron microscopy images of epoxy sections without embedded material also reveal an uneven surface. Let us imagine a grid to be a single sided piece of sandpaper and the section to be a double sided piece of sandpaper. Sandpaper grips another piece of sandpaper much more readily than it does a smoothly polished metal surface. Hildegard H. Crowley, Dept. of Biological Sciences, University of Denver, Denver, CO. 80208 28. If you don't have time to glow discharge clean the grid surfaces, try this little trick.