Catalases are inactivated by sunlight under aerobic conditions. Catalase inactivation by peroxidase has also been reported. The enzyme is assayed by following spectrophotometrically the disappearance of peroxide. Catalase is of interest commercially whenever H 2 O 2 is used as a germicide. It is used in the dairy industry to dispose of H 2 O 2 used in milk pasteurization prior to cheese making.
Microencapsulated catalase is also of interest.
CTR, measure desired amount of mixed suspension, centrifuge to collect enzyme crystals, remove supernatant. Resuspend crystals in one-half initial volume of water, respin. Discard wash supernatant and dissolve crystals in buffer of choice. All preparations are stable for 12 months when stored at 5 deg. C. Lyophilized preparation should be protected from moisture.