This paper describes a new approach to forming bioconjugates that attaches the small molecule to a cross-linking reagent that then reacts with a protein. Biomimetically Activated Amino Acids. Ethyl Phosphate Ronald Kluger, Richard W. Loo, and Vince Mazza, J. Am. Alanyl ethyl phosphate is an activated derivative of alanine that is functionally related to the corresponding aminoacyl adenylate, the initial activated amino acid intermediate in protein biosynthesis. In order to establish the inherent reactivity of these species, the kinetic parameters for hydrolysis of alanyl ethyl phosphate in water at 25 C were determined.
Metal ions catalyze the hydrolysis reactions through complex formation.
These results show that aminoacyl alkyl phosphates are sufficiently stable to be used in water and that metal ions can facilitate their reactions. Destruction of Vitamin B1 by Benzaldehyde. Thiamin is destroyed in reacting with benzaldehyde in neutral solution. The reaction has a puzzling mechanism and biochemical implications. Ruling Out Stepwise Cationic Fragmentation Ian F. Moore and Ronald Kluger.