Histochemical staining of HeLa and BHK cells for beta-galactosidase activity. The transfections were performed in the absence of serum for one hour. The cells expressing beta-galactosidase are stained blue. Colonies of resistant cells were counted 2 weeks post-transfection. 4,766,072 has been issued to Promega Corporation for transcription vectors having two different bacteriophage RNA polymerase promoter sequences separated by a series of unique restriction sites into which foreign DNA can be inserted.
Reagents are related products designed for efficient transfection of eukaryotic cells.
These three reagents are heteromeric lipid compounds containing the same cationic lipid species but formulated with different ratios of cationic lipid to neutral, fusogenic lipid. The optimal reagent for a given cell line is determined empirically. Each of the reagents supports transient and stable cell transfections and performs in the presence of serum. Human Gene Therapy 5, 469. Capaccioli, S. et al.