Bands become to overlap and it is difficult or impossible to find and label each band. Multiplex PCR amplification of mixture K, using four different DNA samples. As depicted also in Fig. 7, 2-3 bands become visible for each product. It is unclear where the extra band originates when only one allele is amplified. Denaturing PAA gels, however, are more expensive, time consuming and might prove technically more difficult.
Primers amplify nonpolymorphic loci.
The sizes of the longest and the shortest product are also indicated. These primers amplify polymorphic loci, and alleles of different sizes can be observed. The shortest alleles of locus 6 and the longest alleles of locus 7 can, sometimes, overlap, making it difficult to assign precisely their origin. The sizes of the longest and the shortest product are also indicated.