Summary In summary, heterologous gene expression in baculovirus systems offers several advantages over E. coli . The viruses are considered safe for laboratory personnel. Insect cells infected with the virus recognize and properly process a variety of recognition signals in mammalian, plant and yeast genes, and provide moderate to high yields of biologically active proteins for study. There are numerous books and articles on baculovirus expression. Invitrogen has established an internet baculovirus discussion group that may be of interest or helpful in troubleshooting.
Many companies now sell baculovirus expression vectors or complete expression systems.
Prepared for the November 1998 OMRF Research Technology Forum.