As little as 10 femtomoles of dinitrophenyl residues contained in a single protein are readily detectable in a 30 second exposure. The OxyBlot TM Kit is applicable to purified or crude protein prepara-tions. Samples may be transferred to either nitrocellulose or PVDF membrane.
Positive control provided in kit allows interblot comparisons.
Fast 1 minute exposure by chemiluminescent detection. Oxygen-derived free radicals have been implicated in various physiological and pathological processes such as aging, apoptosis, cancer, neurodegenerative diseases, chronic inflammatory diseases, pulmonary diseases, and cardiovascular diseases 1 . Proteins are one of the major targets of oxygen-free radicals and other reactive species. The Oncor OxyBlotTM Kit provides a new, sensitive method to detect and quantitate oxidized proteins immobilized on nitrocellulose membrane 4 . Pognonec, P., et al.