This is a unique kit available on the market that will not detect mouse monoclonal antibodies of IgA and IgM subclasses that may be of less interest. However, it will allow a researcher to select only IgG1, IgG2a, IgG2b and IgG3 producing murine hybridomas for further antibody development. The Mouse HybriScreen IgG detection system responds to the need in today's cell culture laboratory for more rapid, sensitive, and standardized immunoassays. The reagents in this package detect primary antibodies of mouse isotypes IgG1, IgG2a, IgG2b, IgG3 origin in one simple, antigen-mediated immunoperoxidase assay. The Mouse HybriScreen detection system is sensitive down to 50 ng, fast and easy to perform.
Purified antigen is absorbed to the microtiter plate wells.
The remaining sites for protein binding on the microtiter plate are saturated with blocking protein solution. This complex is incubated with the antibody secreting hybridoma supernatants or diluted ascites. The mouse immunoglobulins bind to the antigen absorbed to the wells. Signal is detected by incubation with horseradish peroxidase labeled Goat Anti-Mouse IgGs. Blue color is developed in the TMB reaction with peroxidase within a few minutes.