Plastic centrifuge tubes should be discarded after one cycle of ultracentrifugation. The failure rate for used tubes is a hazard which justifies the use of new tubes for each high G centrifugation. They must never be heated because of explosive possibility. Test tube brushes must not be used for cleaning the cup cavities. All traces of detergents should be removed prior to air drying.
Emergency Procedures Turn off centrifuge, notify others in laboratory and evacuate.