Start with about 10 grams fresh needles. Chop into short pieces.
Filter homogenate through several layers of cheesecloth and one layer of miracloth.
Move to orange cap tubes. Incubate at 60oC for 15 minutes. Centrifuge at 5000 Xg for 10 minutes at room temperature. Use table top centrifuge. Hook the precipitate out with a hooked pasteur pipet.