Add loading dye with internal size standard. Four key aspects of mutation detection PCR primer design, PCR protocol, separation gradient, separation temperature DYS271and an unknown gene as examples. SNP by microarray Affymetrix HuSNP genotyping chip. Primarily for linkage studies, also for LOH and association studies. Variants of proteins.
Genetic distance is a function of recombination fraction .
LOD score to evaluate whether the recombination fraction you measured is statistically significant.