For the infrequent user, the AIF provides a completely assisted technical support service. For the trained microscopist, the AIF is an available equipment resource. Significant effort is devoted to training investigators who require microscopy techniques to advance their particular research applications. Electron microscopic techniques include TEM and SEM as well as specimen preparation, including freeze fracture and immunogold labeling. Histology includes cytochemical and immunocytochemical staining and labeling.
Light microscopy includes bright field, dark field, phase contrast, and epifluorescence.
The AIF also provides extensive computer support for image processing and quantitation, and its computers are networked for convenient connection with laboratories throughout the university. Human Genetics and Genomics Many human disorders have a genetic basis, and isolating the genes that play a critical role in the disease process will be a first step toward developing diagnostic procedures and therapies. Some of the facilities currently supported by the program are described here. This facility provides high-resolution chromatography and molecular cytogenetic analysis to researchers working on the discovery and isolation of genes associated with specific forms of genetic and malignant disorders. Molecular and cytogenetic analyses include FISH for mapping and positioning DNA probes directly to metaphase chromosomes or to cells in interphase, and staining and chromosome microdissection for mapping genes and dissecting DNA from regions involved in malignancy.