Principle of the STAT Activation HitKit Products. The STAT Activation assays are based on fixed end-point determinations derived from extensive time course examinations in multiple cell types. Following compound treatment, agonists are screened by immunofluorescence monitoring of the STAT isoform's nuclear translocation. Whole cells are fixed, permeabilized, labeled with a STAT isoform-specific primary antibody, and then stained with a secondary antibody conjugated with a fluorescent dye. STAT activation by nuclear translocation.
Kit specifications are subject to change without notice.
Inc. All rights reserved.