A light microscopic technique in which only a small spot is illuminated and observed at a time. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible. See also scanning technology. Includes CCD cameras, lasers . These methods demand intense computing hardware, software and algorithm development.
Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
Although the visual image of a microarray panel is alluring, its information content, per se, is minimal without significant image processing. The most common current application of image cytometry is for DNA analysis, followed by quantitation of immunohistochemical staining. The use of a photosensitive system for the capture, recording, and retrieval of information associated with an object using electromagnetic energy. This phenomenon is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation in the visible region. Lasers operate in the visible, infrared, or ultraviolet regions of the spectrum.