It hibernates in cold weather. Breeding occurs from December to June.
Internal fertilization occurs.
Females lay 150-200 eggs in a strand that then sinks into the mud. In North America, the range of the Three-toed Amphiuma begins from a point in Southeast Missouri and spreads southward to the Gulf of Mexico. In Texas, it is found only in East Texas. This project funded by the University of Texas College of Natural Sciences and the Texas Memorial Museum . Comments welcome .