Wiest PM, Li Y, Olds GR, Bowen WD 1992b. Inhibition of phosphoinositide turnover by praziquantel in Schistosoma mansoni . Bowen WD 1992c. Strategy of construction of the female-specific library that was used to isolate the cDNA sequences described in this paper. Northern blots showing expression of the p14 egg shell gene in paired females after 6 and 10 days of in vitro culture.
Expression is still clearly visible.
Expression of egg shell genes is decreasing after one day of separation already, and has totally disappeared after three days of separation. PDI mRNA, which is not controlled by the male, shows only a slight decrease in expression during the first three days of culture of separated females. Hypothetical scheme of signal transduction in the female following the stimulation by the male inductive signal. The direct target genes for the signal appear not to be the structural genes in the differentiated vitelline cells of classes 2 to 4. Instead, the signal appears to activate a growth factor that acts as a mitogen stimulating the stem cells of the vitellaria to undergo mitoses and subsequent unequal divisions.