One approach to evaluate morphological data is to digitize images from various sources according to optical density and exploit those images to best automate measurement routines, or maximize contrast to provide high quality figures for publication. Analyses range from the simple counting of cells in specific locations and measuring areas of immunocytochemical reaction product, to the analysis of cell movements by video microscopy. Alternatively, instead of using digital images, tissue elements can be mapped interactively based on their selective appearance in tissue. For live video microscopy, workstation software controls a shutter and frame-grabber to provide image acquisition at specified intervals. For confocal microscopy, images are acquired at neighboring McLean Hospital and brought to the Shriver Center for redisplay and further analysis.
Image analysis presents constantly changing problems.
New biological questions call for multiple and different approaches, and sometimes additional equipment or software.