Note the rapid decrease in peripheral F-actin as compared to control cells in isotonic medium, and the more homogeneous distribution around the cell periphery. A higher value of the ratio indicates increasing intracellular pH. Cells were initially suspended in a medium with 145 mM Cl and 25 mM HCO 3 . For SF9 cells infected with a baculovirus designed to express AE1, when the external Cl is lowered, the AE1 mediates an exchange of internal Cl for external HCO 3 . This raises the internal HCO 3 concentration, and hence raises the internal pH and the fluorescence ratio. This method enables us to observe the effects of an inhibitor on the AE1-mediated transport in a single cell.
Hypotonicity induces L-selectin shedding in human neutrophils.
Role of amino acid transport in regulatory volume decrease in promyelocytic leukemic HL-60 cells. Persistence of external chloride and DIDS binding after chemical modification of Glu-681 in human band 3. Differential inhibition of AE1 and AE2 anion exchangers by oxonol dyes and by novel polyaminosterol analogs of the shark antibiotic squalamine. Kinetics of bicarbonate transport in human red blood cell membranes at body temperature. The asymmetry of chloride transport at 38 degrees C in human red blood cell membranes.