EGF stimulated PC12 differentiation. The switching between growth stimulation and differentiation is considered to be due to the duration of the stimulus. The function of the biosignal conjugate was regulated using conjugation methodology. Whereas proteins have many attractive clinical properties, it is difficult to derive their activities in vivo . However, previously, it was generally believed that biosignal proteins stimulate cells only as diffusible proteins with paracrine, endocrine, or autocrine mechanisms.
Growth factors immobilized on artificial matrices show mitogenic effects.
However, this effect is just a multiplication of soluble growth factor. First, the photo-reactive EGF conjugate was synthesized as follows. Finally, the photoreactive EGF conjugate was purified by ultrafiltration. The cells were washed three times for 5 min each with TBST, three times with PBS, briefly rinsed with distilled water, and then mounted in Vectashield TM mounting medium and observed by fluorescence microscopy. The subcultured PC12 cells were harvested by pipetting.