Analysis of the BSA band after SDS-PAGE should show no evidence of degradation. 1 M Na2CO3 Procedure Adjust Spectrophotometer to read at 400 nm. Na 2 CO 3 to both tubes. Blank spectrophotometer to no enzyme control tube. Read Absorbance at 400 nm.
Longer incubations are necessary if fucose is present on the penultimate sugar.
Prime, S. J. Dearnley, A.M. Venton, R.B. Parekh and C.J. Edge. Oligosaccharide sequencing based on exo- and endoglycosidase digestion and liquid chromatographic analysis of the products. M.R. Wormald and R.B. Parekh. Analysis of glycoprotein-associated oligosaccharides.