During this experiment, monohybrid and dihybrid crosses were conducted with Drosophila melanogaster. Our objective was to examine the inheritance from one generation to the next. We collected the data from the crosses and analyzed them in relation to the expected results. For the monohybrid cross in this experiment, we used an F1 generation, which resulted from the mating of a male homozygous wild-type eyed fly with a female homozygous sepia eyed fly. Males and females are distinguished by differences in body shape and size.
Males have a darker and rounder abdomen in comparison to females, which are more pointed.
At week 0, after being anaesthitized by fly-nap, three males and three females were identified under a dissecting microscope and placed in a plastic vial with a foam stopper at the end. The vial remained on it's side until the flies regained consciousness so that they didn't get trapped by the culture medium at the bottom. We allowed the Drosophila to incubate and reproduce for a week. After one week, the vial contains many larva in addition to the F1 generation flies. Next, we removed the F1 generation flies to prevent breeding between the two generations.