The Cancer Center Core Facilities serve Cancer Center members by providing scientific consultation, technologies, and services that facilitate interaction and advance scientific discovery in a reliable and cost-effective manner. TPF technicians retrieve tissue from operating rooms and, in consultation with a surgical pathologist, provide excess waste tissue to researchers with IRB approval. Solid tumors, normal and diseased tissue, blood, urine, and saliva are available.
Investigators can use our new cryostat and microtome as well.
Banked samples were stored as frozen samples in liquid nitrogen, embedded in OCT and frozen, or formalin fixed and embedded in paraffin. RNA was extracted and subjected to quantitative, competitive PCR using an internal control molecule. However, all of the ovarian cancer samples and all poorly-differentiated endometrial cancer samples showed high levels of AREB6 mRNA regardless of estrogen status. In contrast, AREB6 mRNA was undetectable in well-differentiated endometrial carcinomas. The hypothesis that AREB6 promotes the proliferation of some reproductive cancers is supported.