Specify the organism which the query sequence comes from. This allows the program to choose the corresponding Ig germline gene database for annotating the domains and reporting the top three germline V, two D and two J genes correctly. Limit to this organism when searching nr database Choose an organism to limit you search when the specified database is nr. This option has no effect for Ig germline V gene database. Expect The statistical significance threshold for reporting matches against database sequences.
Fractional values are acceptable.
In addition to finding related germline genes to the query sequence, this program can also match the returned hits from the nr database to the closest germline V genes. Users can specify the number of sequences for which they want this function to be done. Keep in mind that the larger the number you choose, the longer it takes to run the program.