The volume must not exceed 0.1 ml. In priming prior to injecting hybridomas, it appears that 0.1 ml is an adequate dose of pristane. In no case should more than 0.2 ml of pristane be injected. Pristane is a suspected carcinogen and should be used with caution. Ascitic fluid should not be collected more than twice from the same mouse.
General anesthesia is recommended.
If general anesthesia is not employed the needle used for tapping should be 22 gauge or smaller. In vitro production of monoclonal antibodies should be considered. J.R. A retrospective review of lesions associated with the use of Freund's adjuvant. Chapel, H.M. and August, P.J. Report of nine cases of accidental injury to man with Freund's Complete Adjuvant. I. The role of hydrophile-lipophile balance.