Conduct the extractions in a well ventilated area. Peroxides can accumulate in open containers of ether. These are explosive and shock sensitive. Check each container opened for more than 30 days for peroxides. Ether-containing peroxides must be disposed with special techniques.
Electrical equipment is to be grounded.
Extractors should be spark-proof. Make sure all ether is evaporated from the beakers before placing them in the oven to avoid a fire or explosion. Weigh a second subsample for dry matter determination. Extract with five 20 mL portions of deionized water allowing each portion to drain, then insert the paper and sample into thimble. Dry for 5 hr at 100oC. Dry beakers to be used for fat determination for at least 1 hr at 100oC. Cool the appropriate number of fat beakers in a desiccator.