Limited information exists on linkage relationships and chromosomal mapping in Cucumis .
It would be useful, therefore, to obtain reproducible and precise genetic markers which might lead to the characterization of linkage groups and eventually to the formation of a chromosome map of this horticulturally important genus. Mature fruit, seed and cotyledons of two inbred C. sativus var. There were no species differences observed with regard to specific activity within tissues but interspecific enzyme polymorphisms were evident. Enzymes were categorized as to their potential usefulness as genetic markers on the basis of their relative activity and resolution. Green cotyledons were determined to be the tissue of choice for subsequent studies investigating the amount and types of available polymorphic loci.