Gene transfer technology. We invented a negative selection strategy using anti-sense RNA as a negative selectable marker for gene targeting. Future work involves implementing this system to disrupt or replace genes in plants. Specifically those genes which regulate cold and frost tolerance. These AFP have been characterized in winter rye as well as in several bony fish species.
Initial results indicate that the anti-freeze protein confers frost resistance to leaves.
Research will now focus on crop improvement and impact on lipid and carbohydrate metabolism. The hcs gene codes for a Ds RNAse which may serve to endonucleolytically destroy the double-stranded replicative form of many plant viruses. Lipid metabolism and gene expression during senescence and under pathological and abiotic stress in plants. Expression of a synthetic antifreeze protein in potato reduces electrolyte release at freezing temperatures. Purification and characterization of beta-hemolysins from Staphylococcus aureus and Staphylococcus intermedius .