The major by-product of protein breakdown is urea, which is a diuretic. Urea stimulates the kidneys to produce more urine. Along with water, minerals are lost in the urine. This deficiency must be made up by the body's calcium reserve, which is taken from the bones. This tissue breakdown is performed by enzymes.
Enzymes offer an important means of calculating the vital evergy of an organism.
That which we call energy, vital force, nerve energy, and strength, may be synonymous with enzyme activity. Our logic tells us that the buildup and the breakdown of tissues are performed by enzymes. In other words, our metabolism is maintained by enzyme activity. Investigations have shown that in warm temperatures, enzymes are used up more rapidly than in cool temperatures. The prevalent thought here is that enzymes are not actually used up, but many tests have shown that various enzymes are found in the urine after fevers and athletic activity.