V Reaction rate. At high concentration of substrate, approximately V max . At low concentrations of substrate, follows first-order kinetics. V max Maximum rate of reaction. K M Michaelis constant.
Large values indicate substrate bonds weakly to enzyme.
The resulting curve is hyperbolic. A Lineweaver-Burk plot using these same two data sets are also given. For example, in the above graphs, V max for both data sets was set equal to 1.00. This is not at all obvious in the first plot, since the concentration of substrate was not taken to a high enough value. This is common, since the solubility limits the maximum amount of substrate that can be dissolved in solution.