Describes the utility of combining amino acid composition analysis with other physical parameters to identify proteins from two-dimensional gels. The same samples are then further characterized by automated Fmoc amino acid analysis. Software then uses the sequence tag, composition data, molecular weight, and isoelectric point to identify the protein by searching sequence databases. Electrophoresis 17, 573-579. Companion article from the same group emphasizing critical parameters involved in amino acid analysis, such as accuracy, reproducibility, and sources of contamination.
Human keratin is singled-out as a common contaminant.
More from the same group, this one emphasizing the critical first steps sampling handling and acid hydrolysisin the method. These steps can be streamlined to minimize sample manipulation by using a single vial for hydrolysis, extraction, and Fmoc-derivatization. Analytical Biochemistry 237, 146-149. Effect of Polybrene on the N-Terminal Sequencing of Peptides Bound to Polyvinylidene Difluoride Membranes. Addresses the commonly held belief that polybrene is needed to successfully sequence PVDF-bound peptides.