We are using recombinant FIV envelope protein as a probe and as an affinity matrix to bind and isolate cell-surface molecules that interact with the FIV envelope. Specific proteins bind to the recombinant protein, and efforts are under way to identify these molecules. This further characterization will increase our understanding of the catalytic mechanism of this unique pyrophosphatase, and, we hope, will yield generalities helpful to the study of all pyrophosphatases. We hope that all these studies will lead to a better understanding of the life cycle of FIV and that this information will direct the development of effective interventions. Johnson, J.C., Gasmi, M., Lim, L.E., Elder, J.H., Yee, J.-K., Jolly, D.J., Campbell, K.P., Davidson, B.L., Sauter, S.L. Minimum requirements for efficient transduction of dividing and nondividing cells by feline immunodeficiency virus vectors.
Host cells range distinctions and specific cytokine upregulation.