The grouper, Epinephelus coioides, a high value marine food fish, is the most popular marine fish cultured in floating net cages in Southeast Asia.
Fingerlings are wild-caught from the coastal region.
Therefore, there is a great urgency to find ways to reduce mortality in grouper. One possible way of achieving this is through vaccination against vibriosis, a bacterial disease that is very serious in the culture of grouper. Four experiments were conducted to determine the effectiveness of vibrio vaccine on the survival of grouper fingerlings. The first experiment consisted of four replicates, each with 60 groupers of 10-20 g in 4 floating net cages each measuring 1.5m x 1.5m x 2m. Two replicates were controls, of which one was injected with saline and the other was not injected.