Literature abounds concerning analytical ultracentrifugation.
Contained within the facility is a book written by Greg Ralston called Introduction to Analytical Centrifugation published by Beckman. There are several on-line treatments of analytical centrifugation including an overview of sedimentation velocity by Allen Furst from Beckman Coulter. Application information is also available from the Beckman Coulter web site. Sedimentation of molecules is dependent on several factors. External factors include rotational speed, temperature, and time.