A gene is composed of Coding Regions and Control Regions. Experiment showing that Tissue-specific gene expression is controlled by regulatory regions of the gene. Implication of the result is that tissue-specific transcription factors are present in differentiated cells. General Model of Transcriptional Control . Particular combinations of gene regulatory proteins are responsible for initiating and maintaining expression of any particular gene.
External signals can regulate gene expression.
Steroid hormones can enter cells and regulate transciption directly. Stages of Muscle Cell Differentiation in Cell Culture. Evidence supports a myoblast fusion model of muscle fiber formation. Substractive Hybridization Cloning experiments were used to find genes that were selectively expressed in cultured myoblasts, but not in undifferentiated mesoderm cells. Outline the steps you would use to do this.