Uses a translocation channel which is group of proteins in the mitochondrial membranes. Translocation requires ATP and an electrochemical potential and a translocation motor. The precursor is unfolded when it crosses the membrane c. Processing - removing the targeting signal. Carried out by an enzyme - matrix protease in mitochondria and stromal protease in chloroplasts. Sorting - putting the protein in the right place.
Further signals send the protein to the right membrane inside the organelle.
Many mitochondrial proteins are part of a complex. Some parts come from the cytoplasm, some are made within the the mitochondria. Describe the main features of mitochondrial targeting sequences.