Banked samples were stored as frozen samples in liquid nitrogen, embedded in OCT and frozen, or formalin fixed and embedded in paraffin. RNA was extracted and subjected to quantitative, competitive PCR using an internal control molecule. However, all of the ovarian cancer samples and all poorly-differentiated endometrial cancer samples showed high levels of AREB6 mRNA regardless of estrogen status. In contrast, AREB6 mRNA was undetectable in well-differentiated endometrial carcinomas. The hypothesis that AREB6 promotes the proliferation of some reproductive cancers is supported.
Experiments are in progress to define the underlying mechanisms.
American Association for Cancer Research Conference on Gene Regulation and Cancer, Hotsprings, VA, 1998. The Tissue Procurement Facility has provided Dr. Sanders with over 100 tissues during the past 3 years, including solid tumors and normal tissue of the endometrium, ovary, cervix, breast, and prostate. Heterogeneity in therapeutic response of genetically altered myeloma cell lines to IL-6, dexamethasone, and melphalan. In press, 2000. We would not be a Comprehensive Cancer Center without first-class core facilities.