Cloning of plant callose synthase catalytic subunit using EST walking. Functional study of the protein. Cloned the CP genes of PVX, PVY and PLRV Chinese isolates, constructed plant expression vectors containing two or three different CP genes and acquired corresponding transgenic potatoes, some of which were resistant to virus coinfection.
Field tests are under way.
Tested using the synergic antibacterial activities of the modified Cecropin and T7 lysozyme. The two genes were fused with PR1b signal sequence respectively and put in a plant expression vector. Transgenic tobacco was confered enhanced resistance to Pseudomonas solanacearum. Used TMV CP subgenomic promoter, barnase gene and TMV 3 non-coding sequence to construct a vector in which these genes were in antisense form and transform the vector into tobacco. Attempted to produce barnase when TMV infects the transgenic plants, a strategy of artificially induced cell death.