Invitrogen's Echo Cloning System promises just that. Based on the Univector Plasmid-fusion System developed by Steve Elledge at Baylor College of Medicine, the Echo System avoids having to PCR amplify and sequence an insert every time it is subcloned into a new vector. The newly released Echo System combines Invitrogen's proven TOPO Cloning System for five-minute cloning of PCR products with the Univector System to rapidly transfer clones into several different protein expression vectors. Lox P and lox H elements are 34 bp sequences that are recognized by the 35 kDa Cre recombinase. N-terminal polyhistidine and epitope tags.
Individual components are also available separately.
The selection of Echo-adapted expression vectors currently offered allows constitutive or inducible expression in bacterial, yeast, insect, or mammalian cells. If the available expression vectors do not suit your needs, any vector can be adapted into an acceptor plasmid by excising the multiple cloning region and inserting an oligonucleotide containing a lox P or lox H sequence. Thus, when you want to express a gene in multiple systems with minimal effort, the Echo System may be just what the cloner ordered. Inc. All rights reserved. We welcome your opinion.