Mix 5 ug of restriction digested DNA from one person with 5 ug of restriction digested and methylated DNA from another person in a total volume of 100 ul. Shake the mixture in a 1.5 ml Eppendorf tube for 48 hours at room temperature. Shake at minimum setting to maintain emulsion. Incubated at RT for 30 mins. Remove BNDC by passing samples through Spin X columns.
Heat inactivate mut proteins.
Incubate reaction mixture with 10-15 ug BNDC in 0.8M LiCl at RT for 30 min. You can map the genomic location of these DNA fragments by typing with genetic markers or using DNA microarrays. H are available as samples only, please contact Amersham for samples. Microarray Protocols Preparation of DNA Samples for Arraying Prepare DNA samples by PCR or plasmid extraction. Hybridizations Preparation of Microarrays for Hybridization Moisten arrays by placing the DNA-side of the slides over a beaker of boiling water UV crosslink the DNA onto the glass.