The first used base at the 5' end of this reading is at position 1198 in the contig. This typically roughly corresponds to the primer position for this reading in the contig. The last used base at the 3' end of the reading is estimated to be the 329th base of the template. Together with the template lengths this gives us an estimate of how much template we have available for a long gel or for walking. The estimated size for this template is 1250 bases.
Gap is supplied a minimum and maximum size when a reading is assembled.
In this case the minimum is 1000 bases, and the maximum 1500. When we have both the first forward and reverse reads assembled into the same contig we can estimate the real length reasonably accurately. This estimate is based purely on the position of the start of the reading in relation to the start of the hole, and the estimated length of a long gel. The asterisk here marks that this coverage is more than enough to completely solve the problem by plugging the positive strand hole. This is due to the fact that there are no suitable readings within the estimated long gel reading length of this problem.