Principle A whole blood lysing system is used to prepare immunologically-stained leukocytes for flow cytometric analysis.
Lysis is accomplished through the use of buffered ammonium chloride.
This laboratory has obtained satisfactory immunophenotype results on leukocytes obtained from whole blood, bone marrow, lymph nodes, and body fluids using this system. Place 100 l of cell suspension in the bottom of test tube. Refer to protocols on individual specimen types for cell concentration requirements. Add antibody, volume according to vendor, to the test tube. Vortex, then immediately add 2 ml of ammonium chloride and vortex each tube immediately after addition.