It is commonly used for screening RET mutations.
Incomplete digestion is a well-known cause of false results.
Herein, we report another limitation of the method. Genetic study was carried out by both restriction analysis and direct sequencing. Independently, both the novel silent mutation and the missense mutation at codon 883 may disrupt the same Alu I restriction site. Based on the restriction pattern we were able to say that both mutations occurred in the same allele. Restriction analysis is an easy approach for screening RET mutations however it is not enough to assign a final diagnosis.