Life Sciences Division and Center for Human Genome Studies, Los Alamos National Laboratory, Los Alamos, NM 87545.
Individual chromosomes provide the skeletal framework on which genomic data is organized.
As the physical map and an ordered assemblage of molecular clones for chromosome 16 nears completion, we have embarked on the construction of an 'expressed sequence map' of this chromosome. Assuming that there are 100,000 human genes, approximately 3,000 should be encoded by chromosome 16. To this end, we have chosen the strategy of exon amplification to identify expressed sequences on chromosome 16. Our strategy employs 96-well plate pools of DNA from the flow sorted and arrayed chromosome 16 cosmid library as the substrate for exon trapping. At present we have generated 1800 exon clones from 75 of the 150 plates in the chromosome 16 library.