We solve this problem by isolating DNA directly from the environment, so capturing all of the enzymic diversity present in that environment. We then make stable libraries containing the DNA that can be searched many times for different enzymes. We will also make these libraries from environmental samples likely to contain the activity you require. EnviroGene, or the customer, can then search through the huge number of enzymes contained in the libraries. Searches can be based on enzyme activity or, if appropriate, nucleotide sequence based.
Libraries can be phage, plasmid or phasmid based.
Inserts within a two-fold range of your specified optimal size are cloned. Primary titres of the library vary, but are typically greater than 1 million. This can be amplified as required. Libraries can be off the shelf or custom made on an exclusive or non-exclusive basis. You can screen the library, you can tell us how to screen the library, or we can advise on and agree a search method.