General Model of Transcriptional Control . Particular combinations of gene regulatory proteins are responsible for initiating and maintaining expression of any particular gene. External signals can regulate gene expression. Steroid hormones can enter cells and regulate transciption directly. Stages of Muscle Cell Differentiation in Cell Culture.
Evidence supports a myoblast fusion model of muscle fiber formation.
Substractive Hybridization Cloning experiments were used to find genes that were selectively expressed in cultured myoblasts, but not in undifferentiated mesoderm cells. Outline the steps you would use to do this. When MyoD is transfected into non-muscle cells in cell culture, muscle specific genes are activated. 9.9 illustrates the experimental design. Conversion of a fibroblast to a muscle cell by transfection with the MyoD1 gene.