Dye or Stain Mixture is suggested for comparison and identification of unknowns. Do not allow agarose gel to cool too long prior to use as it will begin to set up in the flask and will not pour smoothly. Distribute liquid agarose gel to students in 100 ml units contained in 250 ml flasks to facilitate pouring several gels at one time. Remind students to empty pipets while gel is still warm or it will set up in pipet. Prepare buffer a day in advance and keep under refrigeration.
Gels run best with cold buffer.
Pour buffer into gel chambers for students, to avoid excessive use on their part. Under NO circumstances should a commercial variable powersource be used on which power can be cranked up to unsafe levels.