Windows Tools Restriction Mapping and Analysis DNA sequences can be analysed almost instantaneously with the powerful Digester tool. Publication-quality restriction maps of linear or circular DNA are created on the fly. Appropriate enzymes can be rapidly identified by screening based on name or a variety of properties, including recognition site, fragment pattern, and cut frequency. By adjusting the threshold coding length, identification of bonafide genes is simple. DNA sequences can be translated in all possible reading frames on the fly, to allow identification of a particular amino acid sequence.
Expression has extensive support for both ambiguous DNA and degenerate amino acid characters.
Designing Primers for PCR Primer pairs are computed from the set target regions, then screened against a series of parameters to maximise priming efficiency. The powerful real-time interface allows the rapid identification of the theoretical ideal primers for the region to be PCR amplified. All the typical sequence properties are provided by the Calculator Tool, including molecular weight determination, GC content and absorbance factor calculation, amino acid content etc. Information for developers will be available on our web pages soon.