We have developed a glutamate sensor with micrometer to submicrometer diam. We then affix functional groups CHO to the optical fiber surface by employing a bifunctional crosslinking agent, glutaraldehyde. The amino acids of GDH mols. Optimal immobilization of GDH occurred between 20 and 25 h in the enzyme soln. The immobilized GDH enzyme mols.
M glutamate, and the abs.
Response times of the sensors are fast due to the direct GDH mol. The glutamate sensor is selective and stable. A submicrometer glutamate sensor has been tested. Our glutamate probes could be applied to the study of subcellular level neurophysiol.