3 High-performance RPC columns tend to be efficient, stable, and reproducible. When packed with the same materials, the narrow bore column will require less solvent to achieve the same resolving power and provides four to six times more sensitivity. The selectivity of these columns minimizes eluent or gradient modifications to produce optimal separation. Other column particulars to be considered are matrix particle and pore size and the composition and dimensions of the column material. The smaller the particle, the higher the resolution.
High resolution is a necessity when performing nucleic acid analysis.
Michrom offers the Magic Bullet, using patented column technology combining a continuously tapered PEEK bore with titanium fittings for axial concentration of sample components. As previously stated, interactions with the stationary and mobile phases enable chromatographic separation. Paramount to the delivery of the mobile phase from the reservoir is the pump. An isocratic method uses one mobile phase composition throughout analysis. This is most often achieved by premixing a mobile phase in one solvent reservoir and using one pump for delivery.