The entire underside should become wet with the solution. Sit 5' 6. Remove and place on dry Whatman paper to soak up excess solution. Place on Whatman paper to dry. Bake under vacuum at 80 o for 1-2 hours.
Filters are ready for hybridization.
Autoclaving Method for Colony Lifts provided by Dan Bullard, a.k.a. Lift colonies onto membrane as in standard procedure. Place each lift between sheets of Whatman paper. Go 1 minute on liquid cycle. Remove immediately after autoclave comes down.