The amount of MAB needed varies with the intended use.
Larger quantities are necessary for routine diagnostic procedures and for therapeutic purposes.
Commercial biotechnology companies now handle most of the large-scale production, typically using in vitro methods. Small-scale production in individual research labs accounts for most use of the ascites method these days. Core centers, located at academic institutions, offer a good alternative for researchers needing small MAB amounts. Many are at least partially funded by the National Institutes of Health. Using a core laboratory can provide a more cost-effective way for many investigators to use MABs produced by in vitro methods.