The lysosomes may also be localized by the microtubules as well. It has been demonstrated that the lysosomes are arranged in the perinuclear region of the microtubule organizing center. The Golgi apparatus and endocytic organelles are also located perinuclearly. Microtubules are composed of tubulin molecules which are heterodimers of alpha and beta-tubulin. Microtubules are cylindrical structures in which the tubulin heterodimers are packed around a central point.
Lysosomes are membrane-bound vesicles of hydrolytic enzymes.
They are used for the intracellular digestion of macromolecules in a controlled fashion. The enzymes include proteases, nucleases, glycosidases, lipases, phospholipases, phosphatases and sulfatases. These enzymes all have their optimal activities in an acidic environment. The lysosomes represent one of the many organelles present in the cell, so for simplicity we chose to study it in relation to the microtubule network. Nocodazole acts to bind to the tubulin and prevent the polymerization that leads to microtubule formation.