In addition, we have collated cytogenetic data on sequence-tagged BACs from several other laboratories involved in connecting the realm of cytogenetics to the draft sequence and other maps of the human genome. This website lists the names and chromosomal locations of BACs that give robust signals at single locations in the human genome. This map-integration simplifies the discovery of genes disrupted, deleted, or duplicated by chromosomal rearrangements causing human disease. Clones that cross-hybridize to multiple locations, a feature that complicates their use as a cytogenetic reagent, are excluded. Also excluded are clone where FISH locations are inconsistent with available RH and genetic map colony-purified by streaking to single colony.
FISH also checks for clones that hybridize to multiple locations.
So far, the correspondence between the RH and cytogenetic maps is excellent, with only a few discrepancies. Details of the targeted BAC effort can be found here. This effort identifies BACs for regions of the genome not well seeded with STSs. This effort also tests the randomness of the BAC library's coverage of the genome. Details of the random-BAC effort can be found here .