As the questions being asked and the methodologies being applied in biotechnology get increasingly sophisticated, the purity and quality of materials takes on greater importance. And so it is for some of the uses of isolated DNA fragments. Anyone who has ever run an agarose gel will have no trouble accepting the fact that a band may not be a single DNA species, but rather a collection, albeit a small collection, of similarly-sized molecules. Nonetheless, for some studies, even a small amount of another species can confound the analysis. While gel systems exist that have superior resolution power, they are not always the easiest to work with, particularly in high-throughput situations.
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In addition, many of the kit providers have protocols and documentation of their products on their Web sites. During Detection of Unknown Polymorphisms with Temperature Modulated Heteroduplex Chromatography . Inc. All rights reserved. We welcome your opinion.