Load 20.0 ul of DNA marker in to one well of the gel. Do this by sucking the solution into the pipet tip, placing the tip in the top of the well, and gently expelling the liquid into the well. This will cause the liquid to sink to the bottom of the well. Load 20.0 ul of the uncut plasmid and the restriction digestion. Stain the gel by incubating it for 8 min in an ethidium bromide solution.
Ethidium bromide is very carcinogenic.
Handle this gel only while wearing gloves. Never put unprotected fingers in the gel buffer solution. I will move the gel onto the U.V. trans illuminator and take a picture of it. I will denature the ethidium bromide by placing the gel in potassium permanganate solution for 5 minutes, then discard it. The sizes of the molecular weight standards may be different than in figure 2 and will be given to you during lab.