Through the Microscope Photomicrography, microphotography, photography through the microscope or whatever you wish to call it, is quite different from normal daylight or flash photography to which most are much more accustomed.
Light sources are different, so color balance becomes important with photomicrography.
The illumination is reduced compared to daylight or flash, so reciprocity failure of film is an issue. This is especially true in the case of fluorescence photography where the background is nearly black. Obtaining a good photomicrograph involves setting up microscope illumination to get the best image possible and then taking the photograph with attention to the photographic principles involved. To take a good micrograph, the microscope illumination path must be set up properly and optimized for the particular specimen to be photographed. One should consider using a lens of a higher numerical aperture if there is not enough detail in the image and to get the best resolution.