330 nm is max emission for Trp residues in native folded proteins. Simply the existence of a fluorescence signal indicates the presence of protein. After you have initiated release, describe how you could make use of an anti-DnaK antibody and protein A to see whether radiolabeled luciferase is still associated with DnaK. Go through the individual steps of your designated experiment, ending with the final result. The expected procedure was a co-immunoprecipitation. Incubate complex with anti-DnaK. Add protein A, covalently coupled to Sepharose.
F c domains, so that the complex can be pulled down by centrifugation.
Stain and fix the gel, do an autoradiography.