Nosocomial infections are infections that develop in the course of a hospital stay and were not present in the patients upon admission to the hospital. Rub the swab inside a nostril or in the back of the throat. Smear the swab onto about one quarter of the MSA plate, rolling it around to assure good inoculation. Discard the swab as directed. Observe the plate for typical colonies.
Flame sterilize the loop and allow it to cool.
Flame sterilize the loop and allow it to cool. Turn the plate in the same direction and spread the bacteria from the end of area B across area C. Flame sterilize the loop and allow it to cool. Flame sterilize the loop before setting it down.