However, it contains trace amount of polysaccharidases such as amylase, maltase, and sucrase which can contribute to falsely high glucose levels. The purified enzyme is free of these traces and is recommended for analytical use in the presence of di- or polysaccharides. Considerable work has been done on immobilized glucose oxidase. Tsuge et al . The a anomer is not acted upon.
Dry preparations are stable for years when stored cold.
Solutions are reasonably stable under a variety of conditions. The reaction velocity is determined by an increase in absorbance at 460 nm resulting from the oxidation of o-dianisidine through a peroxidase coupled system. One unit causes the oxidation of one micromole of o-dianisidine per minute at 25deg. C and pH 6.0 under the conditions specified. Handle with care.