Five l of this sample was used for the PCR incubation. A psittacine-specific sequence of cytochrome B was chosen as a reporter target to monitor the presence of nucleic acid and the absence of PCR inhibitors in the extraction mix. The bird died suddenly and only formalin fixed liver was submitted for histological examination. Microscopic lesions included multifocal to confluent hepatocellular necrosis. The final diagnosis was avian polyomavirus infection. .
Hepatocellular necrosis is present.
1B. Eclectus Parrot, liver, ISH for polyomavirus. Polyomavirus nuclear inclusions stain blue. The tissue was flattened and fragmented during cancellation by the post office. In addition, the liver was severely autolytic prior to fixation. The definitive diagnosis was Pacheco's parrot disease.