There are NOT 61 different tRNAs corresponding to the different codons. The end to which the amino acid is bound is called the acceptor stem . Both the anticodon and the acceptor stem are important for this recognition. Uses ATP to complete the ester bond. In prokaryotes, the leader contains a ribosome binding site.
Large numbers imply heavier subunits and therefore faster sedimentation.
The major differences between prokaryotes and eukaryotes are in the initiation stage. IF2 has GTP bound. The initiator fMet-tRNA binds to the start codon, and this complex to the 30S subunit. IFs 1 and 3 leave. The start codon codes for Met.