Organic extraction, precipitation, and two vacuum desiccation steps are used to remove small molecule contaminants from every oligonucleotide. Gel purification is the most economical way to purify oligonucleotides for research needs. Purity confirmed by capillary electrophoresis. There are yield differences with each HPLC purification level. Longer lead times may be required for higher purity levels, especially if process development is required.
Final yields can be sequence dependent.