The methodology for the synthesis of the first strand of the cDNA summarized below is based primarily on this source. This will measure the total radioactivity in the sample. This will measure the radioactivity incorporated into high molecular weight nucleic acids.
Ethanol precipitate with ammonium acetate.
Thaw, centrifuge at room temperature for 10 min in a high speed microcentrifuge. Repeat the precipitation. Centrifuge for 10 min, and dry the pellet. I site in the insert you are studying. Molecular Biology Protocols .