The wand tools automatically outlines structures isolated using thresholding. Results from the most recent measurement are displayed in the Info window. Use Show Results to display a table of results since the last time the Reset command was used. The Analyze Particles command automatically counts and measures features of interest. This requires thresholding to discriminate objects of interest from surrounding background based on their gray values.
Image has two thresholding methods.
For successful thresholding, it may be necessary to use the Subtract Background command to remove the effects of uneven illumination. Images can be calibrated to either spatial or density standards using the Set Scale and Calibrate commands respectively. Editing Image 's tool palette contains several tools used for image editing and annotation. The text tool draws text using the current font, size, style, and foreground color. Click on a color with the eyedropper tool to select a new foreground color.