A needle is inserted into the vein, and the blood is collected in an air-tight vial or a syringe. During the procedure, the tourniquet is removed to restore circulation. Once the blood has been collected, the needle is removed, and the puncture site is covered to stop any bleeding . Cotton or a bandage may be applied to the puncture site if there is any continued bleeding.
Immunofluorescence is a laboratory technique.
The exact technique may vary depending on the specific antibody that is being investigated and between different laboratories. The physical and psychological preparation you can provide for this or any test or procedure depends on your child's age, interests, previous experience, and level of trust. When the needle is inserted to draw blood, some people feel moderate pain, while others feel only a prick or stinging sensation. Afterward, there may be some throbbing . A negative test result is normal.