The results suggested that the membrane strain-field was nominally equibiaxial. The in vitro model of TBI was used to apply quantified deformation parameters to the neural-like cells and monitor relative membrane potential alterations. In conjunction with the EFM, a ratiometric fluorescent dye was used to detect membrane potential changes. A ratiometric fluorescent dye produces a change in fluorescent intensity proportional to a membrane potential change. The small changes observed suggest a slight correlation between membrane potential and deformation parameters.
Further experimentation is necessary to reach some definitive conclusion on the working hypothesis.