LLLT modulates and accelerates physiological processes in cells. We compared regeneration in denervated and innervated amphibian muscle and studied whether LLLT before injury can stimulate regeneration in denervated muscle. Control muscle received red light irradiation at the same wavelength. On d9 postdenervation, cold injury was performed on the site of irradiation of both groups of muscles. On d14 postinjury, all muscles were removed for histology and histomorphometry of mononucleated cells, myotubes, and young myofibres in the regenerated zone.
Early regeneration can also take place in skeletal denervated and injured muscles of amphibians.
The kinetics of the regeneration process are identical in denervated and innervated muscles. The three-layer pattern provided the best healing of the entire tear. LDF, tensiometry and hydroxyproline analysis correlated well with histopathological examination. LDF, a more rapid, less invasive and painless procedure, can replace tensile strength measurement or hydroxyproline analysis to assess the progress of teat wound healing. RJ Gomezvillamandos, Univ Cordoba, Fac Vet Med, Dep Vet Clin Pathol, Avda Med Azahara 9, E-14005 Cordoba, Spain.