The yeast cells of P. marneffei divide by fission, but not by budding. Histoplasma capsulatum predominates at the central portion of the lesions. Thus, the selection of the tissue section to be excised for histopathologic and mycologic examination is of particular significance. In contrast to Histoplasma capsulatum , Blastomyces dermatitidis is located more at the periphery of the infected lesion, rather than the center. The buds are attached to the mother cell with narrow necks.
Granule formation is typical.
Chlamydoconidia may be formed. Phaeoid hyphae may also be observed. Fontana-Masson Chromaffin reaction probably oxidizes melanin or melanin precursor as it reduces silver. Cell walls black. Background pale pink.