A few days later, a virus was isolated from sputum. It passed through a 300 nm filter, but not through the 50 nm or 100 nm filters. The virus was BUdR resistant. Replication of RNA viruses is not affected by it. The virus was found to be sensitive to chloroform.
Enveloped viruses have lipids, therefore are susceptible to chloroform, ether, and other solvents.
On Feb 15, the lab collected an additional serum specimen. However, in the presence of specific antibody to the virus, HA is blocked. This phenomenon provided the basis for an assay used to detect and quantitate specific antiviral antibodies in serum. The assays shown below were done on the serum specimens from the patient. E. None of these 7.