Max emission changes because Trp are removed from hydrophobic environment. Justify your answer. Describe briefly the principle of DsbA action. AP has disulfide bonds in oxidizing environment, which stabilize the enzyme. DsbA catalyzes the formation of these S-S bonds through direct interaction.
Forms disulfide without external redox agent.
Can act as oxidant because of internal destabilization S-S bond. Choose one of these techniques and explain its principle. Weakly - elute first. Separation principle size. Column beads with pores of defined size.