Each lot of antigen coated plates is accompanied by information provided by the supplier, including their Lot No., storage instructions, antigen purity analysis, conjugate specifications for analysis and reactivity against a panel of specific reference antisera to document specificity of the antigen for the homologous antiserum. These analytic lot specifications are archived as a permanent documentation of antigenic validation. The teflon is hydrophobic and prevents diluted sera in one well from running across and contaminating the adjacent. Each well contains tissue culture cells with both virus infected and non-infected cells. Moreover, staining of any given test serum must conform in similarity to the staining pattern observed with the positive control serum that accompanies each test run Each new lot of antigen is validated for use in IFA tests.
Information provided by the supplier for each lot includes their Lot No.
Most such procurement is accompanied by Lot No. related validation data attesting to storage conditions and performance characteristics against homologous antigens. Validation data is reviewed at the time of receipt and the Lot No. with accompanying raw data records for each run are archived as permanent records. Thank you for familiarizing yourself with MHV surveillance and detection programs at Taconic. We hope the review can answer some of the questions you may have about these programs. Any further questions or comments you may have about the review or about the status of specific production units at Taconic, is welcomed.