Additionally there were 49,424 BAC-end sequences. The sequence of the ends of 92,000 BACs should be completed by the end of the year. It is important to keep nucleation points to a minimum on each chromosome. The nucleation points must be part of a contig. A variety of methods, including verification of marker restriction fragment size, fingerprinting, use of multiple markers, and end sequences, are required for BAC validation prior to sequencing.
Genetic mapping is required to confirm chromosomal assignments.
We learned that positive funding decisions for both Thailand and India's participation in the project are immanent. DNA markers have been genetically mapped. They constructed an Sau 3AI partial PAC library of 71,000 clones with an average insert size of 112kb. Their current goal is to supplement the physical map with PACs. The current method is to use DNA probing or PCR to map the PACs with mapped DNA markers or with ESTs.