DNA-PK DNA dependent protein kinase. Dominant negative Defective alleles of genes which produce forms of the protein give phenotypic effects even when heterozygous. Such alleles are presumed to produce enough non-functional form of a protein to interfere with the functional form produced by the wild type allele. EAE e xperimental a utoimmune e ncephalomyelitis. Animal model for the human disease multiple sclerosis.
Individuals develop antibodies to myelin basic protein.
Results in demyelination of neurons and paralysis. The DNA is radioactively labeled and then mixed with the protein sample. The mixture is then subjected to electrophoresis. The DNA alone has a given mobility, but if a protein binds to it, the complex is larger than the DNA alone, so the mobility increases. A mobility shift is detected.