Animate animates the images in a stack at a rates up to 100 frames per second. Reslice reconstructs a new 2D image perpendicular to the plane of the slices in a stack. Project does volume rendering, useful for visualizing the internal structures of 3D images. Commands not in the Stacks menu, except for Open, Save and Export, have no knowledge of stacks. For example, the Invert command only inverts the current slice, not the entire stack.
Macros are available, however, for performing various operations on all the slices in a stack.
An image requires one byte of RAM for each pixel plus a few thousand additional bytes for data structures containing information about the image, such as color tables and calibration. For example, a 512 X 512 image requires 262,144 bytes, or 262K, where 1K equals 1024 bytes. To see how much RAM an image uses, use the Get Info command, or pull down the Windows menu, which shows the size of all open images. The number and size of images that Image can work with depends on Image 's RAM allocation. The default size of these buffers is 300K. This can be changed using the Preferences command in the Options menu.