This work describes the effect of the green tea extract on leukocyte transmigration using a modern static cell co-culture system. This article is published in English. Green tea is widely used in Asia and has also become popular in Western countries. The influence of green tea extracts on leukocytes is not well understood.
Leukocytes play a crucial role in the process of inflammation.
They migrate from the intravascular space into the tissue to attack micro-organisms. The aim of the current study was to investigate the influence of epigallocatechin gallate on leukocyte transmigration through endothelial cell monolayers and thereby evaluate its potential role in the inflammatory process. Human umbilical vein endothelial cells were cultured on microporous membranes to achieve a monolayer. Freshly isolated neutrophils from healthy subjects were measured with a migration assay. This is similar to the situation after resorption in-vivo.