Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. The P-lacW construct we mobilized contains components useful in the cloning of DNA adjacent to the insertion site. Restriction enzymes recognize and cut DNA at sequence specific sites. Typically, a series of 6 nucleotide bases are recognized by a given restriction enzyme and the DNA is cut either within this specific sequence or immediately adjacent to the site. The mini- white gene is required for deposition of pigment in the adult eye.
Enhancers usually function in a tissue specific and stage specific fashion.
Thus, movement can be monitored by changes in eye color. After discovering the insertions in grooved and margin , molecular genetics allowed for the cloning and analysis of these two genes. These genes were cloned with the use of the plasmid rescue technique. Using plasmid rescue we have cloned DNA for the insertion sites of two alleles of margin and one allele of grooved . We brought the P-element and the enzyme transposase together by crossing two different strains of flies.