Here, the polarity of a T cell and its activation with a bead coated with anti-T cell receptor antibodies is revealed. The cellular microsurgery can be performed on living cells and combined with confocal microscopy to visualize and monitor the laser microbeam effects. Inset photomicrograph represents the same cell where four of the nine nuclear bodies have been ablated. Opto-poration of DNA and proteins is presently being explored. Arrow indicates the laser-targeted area outside of the cell.
Fluorescence indicates probes that have entered the cells.
Use for detection and characterization of tumors and dosimetry planning for phototherapeutics.