In this example, 22.2 m l would be needed. Place approximately 40 ml of de-ionized water into a beaker and add 22.2 m l of the ethyl ester. This will take several drops. Remember to short the volume of the rest of the KH buffer by at least the volume used to hydrolyze the ethyl acetoacetate. The vendor of the algal FA's should provide an analysis of the mixture so that the molecular weight can be computed from fractional contributions of each fatty acid.
Instructions continue below.
Start the hydrolysis of the ethyl acetoacetate first, since this will take 2 hours. Sprinkle the BSA on top of the water. Do not try to stir, just let it slowly dissolve over about 20 min. Use a 1000ml graduated cylinder. Do not start the preparation of the FA's discussed below until the BSA has completely dissolved.