As a control, shown in Fig. This could be due to the fact that, when Glu was almost depleted, the cells had switched utilizing radiolabeled naphthalene. Once such a mutant is obtained, it is expected to be much more effective in degrading toxic compounds in nature. Further studies in this regard are currently underway. The catabolism of hexose sugars in pseudomonads is very complex.
Glycolysis is the central pathway of all intermediary metabolism.
It has been found that a simple hexose can be catabolized by alternative routes in a microorganism. In the case of pseudomonads, common hexoses are predominantly metabolized by the ED pathway. Therefore, by blocking bottle-neck enzymes such as edd and eda , a P. putida strain can be impaired in its utilization of different sugars. ANL was grown on glucose and aniline, the sugar was utilized first, and aniline was utilized only after much of the glucose carbon was converted to CO 2 . Therefore, glucose can act as a carbon catabolite repressor for aniline degradation.