Computers are evolving from calculators to all-round data processing and publishing machines, and you will be pleased to have GeneBase print out the most complex vector maps and sequence layouts with an unparalleled publishing quality. Automatic Sequence Reading Input of large amounts of sequence data from gels is an uninteresting and laborious task which, if possible, can better be left to a computer. GeneBase provides an algorithm for automatic lane-finding and sequence reading from gels scanned into TIFF files. The probability of the predicted sequence is indicated with a colour code for each nucleotide found and ambiguous regions are reported. Visual interpretation is made easy by simultaneous display of the densitometric curves and the corresponding zoomed gel area.
Manual correction is possible on the densitometric curves or directly on the zoomed gel image.
Databasing and Sequence Editing GeneBase is a versatile multi-user system, in which each user defines his own databases and customizes all settings. Each sequence is described by up to four custom information fields. Lists of sequences, e.g. for multiple alignment, clustering or consensus determination, may be saved to disk. Formats from important databases such as EMBL and GenBank can be directly read. Sequence editing tools include a variety of useful features, such as voice reading, gel reconstruction, subsequence and restriction enzyme search, search for likely subsequences with adjustable stringency, invert complement and translate functions, etc.