Specific enquiries about the organisation of the Biotechnology Program can be made to the Chairman of the Departmental Teaching Committee . During the course the student will have direct access to the most important databases on the Internet. Homology - function and identification. Access to netbased databases. Searching the world wide web.
Emphasis is on mass spectrometry based analytical strategies and techniques.
Sample preparation methods for mass spectrometry Computational data analysis, biological databases and bioinformatics. The course consists of a theoretical part where the methods used are discussed, and a practical part during which the students will purify a protein. The methods used include cell organelle separation by centrifugation salt precipitation ion exchange chromatography gel filtration protein determination photometric enzyme assay. The participants are expected to document purity and to determine molecular weights using gel electrophoresis. The recombinant protein kinase is purified and biochemically characterized.