MacConkey plates were prepared for parallel determinations using standard plate count methods. All dilutions were made with sterile 10mM phosphate buffer. Take 25 g of meat sample and transfer to sterile ziplock bag containing the 225 ml of the pre-warmed media. Each meat sample was prepared in duplicate. All samples were placed on a rotary shaker in a 40 C incubator for 4 hours.
G beads into the face of the pre-wet filter.
Let the filtered antibody bead mixture sit at room temperature in the dark for 15 minutes. Filter the 5 ml solution through a 40 micron cell strainer unit into a 50 ml sterile test tube. Remove 4 ml of the filtrate to a 15 ml test tube and add 200 l of the antibody bead mixture to this sample. Rinse the cell strainer with sterile distilled water and retain. Vortex and incubate at 37 C for an additional 2 hours with rocking.