Vinculin, purified from human uterus, was used as the immuno gen. Sigma Stock No. The antibody reacts with the 116 kD vinculin band in immunoblotting. The product reacts with vin culin of many species.
Good reactivity is obtained with human, bovine, chicken, dog, rat, mouse, turkey and xenopus.
The antibody shows cross reactivity with smooth muscle metavinculin. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. The sequences of chicken, nematode and human vinculin have been determined. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the B-subunit of integrin binds to talin.