The highest peaks are the most hydrophilic and therefore likely to be more antigenic. This means that it is easier to make a monoclonal antibody specific to this region of the protein. Based on the plot, I would try to make an antibody to the region of the protein at either amino acid number 1100 or amino acid number 1400. Amino Acid Sequence Homology. This is an illustration of amino acid sequence homology in myosin for the species chicken, Drosophila, C.Elegans, human, and mouse.
Highlighted regions indicate sequence conservation.
As illustrated in the figure, there are numerous regions where sequence similarity is conserved, as in amino acids 730 to 740. However, there are also regions where there is little to no sequence conservation, as in amino acids 810 to 820. This figure is not very useful in determining the percentage of homology between these sequences, however. For this reason, we will use a phylogenetic tree analysis. A phylogenetic tree showing degrees of similarities between myosin amino acid sequence in the five chosen species.