One or more shutters are important to minimise photobleaching if you using UV excitation, or if you need to capture separate fluorescence and transmission images under software control. Please see our Omega Optical page for details. Aperture For most applications, it will be necessary to adjust an aperture so that the photomultipliers see only the appropriate part of the specimen. The aperture normally mounts on the side camera port of the microscope. A video camera allows the operator to see exactly what is being framed.
Details are on our beamsplitter page.
Allows a dichroic mirror to separate emission wavelengths from the camera illumination. Allows a second dichroic mirror to be used if required to separate dual emission bands. For details, please see our beamsplitter page. These give good sensitivity for fluorescent photometry work, at a moderate cost. Video Camera A video camera is essential to see exactly what is being framed by the adjustable aperture.