Embedding in plastic provides many advantages to the histotechnologist. Thinner sections can be made providing improved detail. Better support is given to cellular components, offering improved morphology. Short, straight-forward protocols are available giving minimum processing times.
Improved chemistry provided by kits gives uniform results and a wider range of application.
Fixation of the biological samples can be done in any way appropriate for the work you are doing. Try to keep the sample size small using 10mm x 10mm x 2mm as a maximum. Dehydration for GMA embedments does not have to be complete because of GMA's miscibility in water. A short defatting step can help with infiltration. If desired, submerge the sample in acetone for 10 minutes.