Describe the major characteristics of the genetic code . Define the terms degenerate, sequential, triplet, and nonoverlapping as they apply to the genetic code. Describe the colinear relationship between the sequence of DNA in a bacterial gene and the sequence of the amino acids in the protein it encodes. Define recombinant DNA . Describe the important features of restriction enzyme DNA substrates and the reactions catalyzed .
Gel electrophoresis is essential to the field of recombinant DNA technology.
Know why this method works and how to predict the results and read DNA sequences. Know that DNA can be synthesized in the test tube for use in recombinant DNA technology. Outline the major steps in cloning a DNA molecule . Describe the reaction catalyzed by DNA ligase . Compare the properties of cloning and expression vectors used in procaryotes vs eucaryotes .