This presentation discusses the integration of biotechnology, electro-optics, and laser technology, which have recently opened new horizons to the development of advanced biosensors for industrial, environmental, biomedical, and biotechnology applications. A new generation of biosensors discussed in this presentation uses antibody and DNA probes.
High sensitivity is provided by laser excitation and optical detection.
The FIS device utilizes the back-scattering of light emitted at the remote sensor probe. A single fiber is used to transmit the excitation radiation into the sample and collect the fluorescence emission from the antigen. The laser radiation reaches the sensor probe and excites the BaP bound to the antibodies immobilized at the fiberoptics probe. The excellent sensitivity of this device illustrates that it has considerable potential to perform trace analyses of chemical and biological samples in complex matrices. Probe recognition is based on the molecular hybridization process, which involves the joining of a strand of nucleic acid with a complementary sequence.