Since the labile pool of Fe is most likely the source available for catalysis of oxidative damage. We are also developing a system for the measurement of a labile Fe pool in the cytoplasm utilizing a fluorescent dye, calcein. Addition of ascorbic acid has dramatic effects on the size of this pool. We are also examining the effects of overexpression of H or L chain on the dynamics of this pool. We have found that low doses of UV light causes a dramatic increase in ferritin synthesis in lens cells.
Higher doses are toxic.
Intriguingly, UV light and ascorbic acid have additive effects on the synthesis of this important antioxidant protein. We are also examining the effects of UV light on Fe metabolism within these cells. This part of the project is just taking off. Dr. Lloyd Fleisher We have collaborated with Dr. Fleisher on his studies of the influence of cytokines on the uveitic response. Please see his website for more information.