Optimization is critical but not always easy to do. Similar time-consuming tests may also be required for denaturation temperature optimization. The MJ Research Gradient Feature allows you to perform optimization reactions in a single experiment by using a range of temperatures simultaneously. With the new Thermal Gradient Software, you can program a temperature gradient up to 24 C across a 96-well sample block. And the gradient feature works with calculated sample temperature mode, so you can generate fully optimized programs in a single step.
Advanced features make optimization easy.
Dynamic ramping means that the temperature gradient forms during ramping. All wells come to the designated temperature at the same time, making the incubation period consistent among all samples in the experiment. Note too the even spread of temperatures, which allows for the greatest quantity of useful data. Note especially how all four cyclers have temperatures exactly where the calculator predicts.