The magnetically labeled cells will remain in the column, while the non-labeled cells and sample particles will elute. The labeled cells will then be eluted from the column when the magnetic force is removed. IMS will be beneficial for the rapid detection of Listeria sp. First, the beads act like magnetic antibodies, so the labeling will be complete within minutes. Also, the beads form a colloidal suspension so they will not precipitate or aggregate.
Immunomagnetic separation shows promise as a technique for the rapid detection of Listeria spp.
Their work showed no significant differences in the quantity of immunomagnetically recovered L. monocytogenes compared to a standard plate count technique. This group was able to use IMS for isolation of Listeria sp. Once the magnetically bound Listeria spp. As a more rapid alternative, a probe measuring electrical impedance of a mammalian cell culture infected with the captured Listeria cells will be employed. A cell and its surrounding environment act like a simple circuit, as shown in Figure 1.