Thus the in vitro virus is an in vitro alternative to phage display for linking genotype and phenotype in molecular evolution studies. In this multienzyme pathway, protein substrates are targeted for degradation by ligation to ubiquitin and subsequent proteolysis by the 26S proteosome. The regulatory subunits are degraded through an allosteric mechanism requiring an elevation of cAMP coincident with the induction of expression of a ubiquitin carboxy-terminal hydrolase. A sensor for DNA damage critical in skin cancer etiology. Cells that suffer DNA damage induced by UV light can either repair the DNA or undergo p53-dependent apoptosis to eliminate precancerous cells in the skin.
Fas ligand is a pro-apoptotic protein.
Cholesteryl esters serve as the intracellular storage form of cholesterol. Researchers in this paper tagged the ACAT-1 protein in order to study the topology of the tagged proteins expressed in Chinese hamster ovary cells. The plasmid constructs were transfected into mutant CHO cells lacking endogenous ACAT and the expressed protein was examined by immunocytofluorescence. No. 5,487,993 and European Pat. No. 5,283,179 and other patents.