Basic fuchsin binds to negatively charged groups in bacteria. This method does not use phenol and has been provided by Roy Ellis , Principal Hospital Scientist, Histopathology, The Queen Elizabeth Hospital, Woodville, South Australia. The two solutions can be kept as stock solution and mixed immediately before use. 3 ml Make up the alcohol solution then add the concentrated acid. Dehydrate, clear .
Acetic acid is a strong irritant and a corrosive.
Use a fume hood and wear protective clothing. It is an inflammable powder and should not be handled close to heat or a naked flame. It is a potential carcinogen and an eye, skin and mucous membrane irritant. The vapour is heavier than air and can travel for a considerable distance along the ground to a source of ignition and flash back. Ethanol is regarded as one of the safest industrial solvents.