Over the next months, this web site will be fleshed out with gel images, databaseses, and links to other pertinent sites. For methods, see refs. Gel Images 1,014 spots have been indexed, and 151 spots have been incorporated into a quality-controlled database for analysis. The indexing was done on a master gel obtained by electrophoresing a mixture of cell types. A central portion of the master gel image is shown below.
Labels indicate spots presumptively identified to date.
This program is demonstrated for the above master image and 2-D gels from MCF7 breast cancer cells. Additional gels from the database will be added over the next months, along with comparison gels run on the same cell lines but in different laboratories. More information on this program, including a reference manual, can be found through the Flicker home page . A demonstration is currently operational for plasma protein 2-D gels, but the master gel shown above, along with others from the NCI drug discovery cell set, will be available soon. This page is under construction, but see Li, et al.