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A Phoma sp. Water activity and temperature and their interactions were significant factors affecting growth of the Phoma sp., with optimum conditions of 0.998-0.980 aw and 25 deg. Squalestatin production was similarly influenced by aw, temperature, time and their interactions. The optimum temp. The ratio of S1 and S2 produced by the Phoma sp. was influenced by aw and temperature, with highest values at 0.99-0.98 aw and at 15 deg.