Expression of the reporter will be regulated by local enhancers. Contrasts with classical genetics, in which a phenotype is first identified and the corresponding gene is then discovered. Bind to ribosome complex at the P site. Single nucleotide polymorphism. Can effectively be a mis-sense or nonsense mutation if it occurs in an open reading frame.
Eukaryotic proteins are often modified post-translationally.
Prokaryotes lack the ability to perform such modifications. The helper phage provides the polymerase required to recognize the phage origin of replication. DNA sequence containing the recognition sequence bound by the bait upstream of an enzyme such as beta-galactosidase or green fluorescent protein. Interaction between the two proteins can be assayed by measuring the expression of the reporter. Also accepted an explanation that the two-hybrid system allows measurement of the strength of interaction.