Unlike many marsupials, the Short Tail Opossums breed any time of year. They are introduced ovulators. These opossums are sexually mature at 4.5 months. I introduce the pair and keep them together for 12 days. The female cycles between the 8th to 12th day once the male has been introduced.
Gestation is fourteen days.
Litter size ranges from two to thirteen babies. On the average, ours have 8 to 10 pups. When the babies are born, the mother will spend the majority of her time in her nest box and may not come out for days. The babies will stay attached to the nipples for one month. When they are about four weeks old, the babies will start to wander about the nest.