Place four-lumen tubing in exit slot on right side of machine. Rotate centrifuge several times to ensure tubing does not twist and upper bearing remains in place. Close centrifuge door and cover. Press l Key to select ELP tubing set. Close both saline lines.
Close roller clamps on access and return lines.
Connect AC line to anticoagulant container, and place AC line in AC level detector. Fill drip chamber half full. Connect inlet and return saline lines to same saline container. Using aseptic technique, clean injection port before inserting metal spike into it. To prevent metal spike from falling out, tape it to the saline container.