With the completion of the working draft for the human genome, aligning ESTs to the human genome has become a practical and powerful strategy for gene prediction and genome annotation.
ESTs are like snapshots of the transcribed genome from various tissue and developmental stages.
These transcript variations are usually difficult to characterize using statistical gene prediction or comparative genome analysis methods. My current research project is focused on using an ESTbased approach to reconstruct and characterize primary and alternative transcripts. Aligned EST Sequences. GR, journal article, talk - Kan, Z., W. Gish, E. Rouchka, J. Glasscock, and D. States. Using Genomically Aligned EST Sequences.