A needle is inserted into the vein, and the blood is collected in an air-tight vial or a syringe. During the procedure, the tourniquet is removed to restore circulation. Once the blood has been collected, the needle is removed, and the puncture site is covered to stop any bleeding . Cotton or a bandage may be applied to the puncture site if there is any continued bleeding.
Immunofluorescence is a laboratory technique.
The exact technique may vary depending on the specific antibody that is being investigated and between different laboratories. No special preparation is necessary. The physical and psychological preparation you can provide for this or any test or procedure depends on your child's age, interests, previous experience, and level of trust. Afterward, there may be some throbbing . It may also be used to detect and quantitate antibodies against Coxiella Brunetti, Rickettsii, Colorado tick virus, and other organisms.