However, not all vectors follow the same sequences and Universal priming sites may be altered. One recent case showing this is a newer vector which has a T7 priming site, but has 2 different bases at the 3 prime end where sequence extension begins, Therefore our T7 primer will not work for this vector. Although we do not know the sequences of all vectors. No it shouldn't. Actually the Applied Biosystems model 3700 capillary sequencer is an instrument made for large genome centers and not core facilities.
Capillary electrophoresis requires that the templates are absolutely clean.
The 3700 CE is more sensitive to template and primer salt contaminants than the model 377 slab gel system and there is a higher number of sample failures. In addition, the matrix used in the capillary has a resolution capacity of a 500 base read length and the model 377 slab gel system has a capacity of a 900 base read length. And finally, the 3700 CE has 30 percent down-time and is difficult to repair. Generally, the chemistry of automated synthesis has been well optimized. If the template has difficult GC rich areas or secondary structure, desalting can help but still may not be necessary.