For your convenience, we accept Mastercard, VISA, Discover, and American Express credit cards. Effect of Pore-Size, Buffer, pH and Column Chemistry on Peptide Purification Different C18 Bonding Chemistry Provide Selectivity Alternatives.
Diversity is an innate characteristic of peptide structures.
Even closely related peptides, for example target product and contaminating side products from a synthetic process, will typically have chemical differences at multiple sites consisting of various hydrophobic, polar, and charged acidic or basic constituents. This remarkable diversity is what allows peptides to exhibit a wide range of biological activities. It also provides chromatographers with the ability to change selectivity and control resolution in reversed-phase separations to a much greater extent than is normally possible with smaller, simpler molecules. Providing tools and understanding to make this possible is one of Vydac's missions. The chromatograms of Figure 1 show separations of the same synthetic peptide mixture on two different reversed-phase columns under identical conditions of flow, temperature, and organic solvent gradient.