The triangles in the lower part of the plot corresponding to the bands in the standard lane. Using the data obtained in the previous step, bands in the sample lanes can be normalized. Linear interpolation is used between standard lanes to estimate the position of the standard bands in the sample lanes. Image produces migration distances for each band by interpolation between the standard bands and can optionally provide fragment size information if a standard is available. This information is transformed using a logarithmic scale for normalization to ensure greater accuracy.
Image can also provide a normalized image of the gel.
A cubic spline is fitted through the pixel intensity values and a normalized image is extracted. This is even capable of converting an ABI gel into a conventional gel image.