Work-Up A A thorough history and clinical examination remains the cornerstones of a reliable allergy diagnosis.
Diagnostic tests are to be used as adjuncts.
Experience is needed for performance and interpretation of SPT reactions. Top E Serum samples should consist of 2 ml of serum. Sera are held for 2 weeks at the laboratory, should individual RAST testing be needed after a positive Phadiatop, total IgE or RAST multi-allergen screening test. It is recommended that a RAST for the specific allergen be performed prior to immunotherapy, even with a concordant clinical history and SPT results. This is especially true for grasses where the RAST will assist in identifying the important grass allergen of the SPT grass mixture, which should be included in the immunotherapy vaccine.