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Background noise can obscure the true sequence data.
Dirty DNA templates are the most common cause of noisy data. Some template preparation procedures are not compatible with fluorescent sequencing. A template that gives poor sequence is usually said to be dirty if it contains some impurity that inhibits the sequencing enzyme or that inactivates some other reagent in the sequencing reaction. Templates that produce poor data can sometimes be purified to a quality that yields good data. Please contact us if you require information on template clean-up techniques.