We also extract protein from nephron segments and identify proteins expressed in the cells by immunoblotting. Using these approaches, we are studying the effects of dietary salt and regulatory hormones on the relative expression of ion transport molecules, adrenergic, dopaminergic, and prostaglandin receptors, as well as intracellular mediators, such as the isoforms of protein kinase C. Recent Publications 1. Kruh ffer M, Kuhn M, Schafer JA, Sun D and Schlatter E. Effects of sodium nitroprusside in the rat cortical collecting duct are independent of the NO-pathway. Schafer JA, Watkins ML, Li L, Herter P, Haxelmans S, and Schlatter E. A simplified method for isolation of large numbers of defined nephron segments. Microtubule disruption prevents an AVP-stimulated chloride secretion but not sodium reabsorption in A6 cells.
Dopamine inhibits vasopressin-dependent cAMP production in the rat cortical collecting duct.
Dopamine D4 receptor isoform mRNA and protein is expressed in the rat cortical collecting duct.