Southwestern Mesic Forest, Oak Savanna and Prairie Upland-southern zone. From the junction of U.S. Hwy. 14 and the Wisconsin River just east of Spring Green, go west on U.S. Hwy. 14 1.75 miles to Davies Road, then north 0.75 mile to Jones Road, then east 0.25 mile to a crushed stone access road next to a residence, then north on access road 0.1 mile to a parking area. Spring Green Reserve features many animal species that utilize a variety of somewhat disturbed plant communities.
Adjacent lands have excellent sand prairies, but the natural area is situated on an old turkey farm.
Debris from the farm still litters the ground but is left untouched because it provides shelter for snakes, lizards, and voles including such rare species as the six-lined racerunner and blue racer. The invertebrates, however, are the most unusual of the Spring Green fauna. Several of the spiders and insects are not known from any other site in Wisconsin. Of special interest are the black widow and several wolf spiders, five species of cicada, eight species of tiger beetles, and predatory wasps. The large pocket gopher population has created patches of open ground where short-lived plants grow.