Pernicious anemia leads to a general muscular weakness and signs of deterioration of the central nervous system. Skin pallor, heart palpitations, and eventually more severe heart disturbances are also symptoms, as are a sore, red, inflamed-looking tongue which eventually becomes smooth and glazed. The first signs of biotin deficiency are dry, peeling skin and mental depression. Other possible effects are muscle pain, extreme fatigue, nausea, and distress around the heart. As a coenzyme it is required for the utilization of sugar and amino acids.
Folic acid is stored primarily in the liver.
Less severe deficiency result in fatigue, dizziness, shortness of breath and a grayish-brown skin pigmentation. Para-aminobenzoic acid and choline cannot be utilized without it, and it is vital to the proper functioning of the adrenal glands. Deficiencies of this are fatigue, abdominal pains, cramps, and nausea, irritability, depression, constant colds, and paresthesia of the hands and feet. As the Pantothenic acid deficiency continued the symptoms got worse and after six weeks could result in becoming very ill. It is also associated with hypoglycemia.