Experimental Virology is designed for students preparing for a research career in virology or related research with proteins and nucleic acids. Lecture topics include laboratory safety, cell and tissue culture, propagation and purification of virus stocks, principles of centrifugation, column chromatography, immunological techniques, protein analysis, nucleic acid methods, x-ray crystallography and electron microscopy, photographic techniques, and an introduction to available computational resources. The emphasis of this course is on the practical laboratory application of theoretical scientific principles. Offered fourth quarter, even years. This course covers the molecular aspects of pathogenesis of virus infections of animals and plants.
Emphasis is placed on infections of animals.
General concepts of viral pathogenesis as well as specific examples of different mechanisms of viral pathogenesis are discussed. The first part of the course features an in-depth discussion of the general mechanisms involved in pathogenesis. Specific viruses for which detailed analysis at the molecular, genetic and immunologic level have been possible are used to illustrate these general mechanisms. The second part of the course is a comprehensive review of a limited number of viruses to illustrate different mechanisms of viral pathogenesis of different organ systems. Examples from either human or animal infections are used.