Construction of viral vectors with altered specificity. Role of TSLC in human cancers. Techniques involve recombinant DNA technology including site-directed mutagenesis, expression of proteins in animal cells, in vitro transformation and tumorigenesis in animals. Mutations in a carboxy-terminal region of vesicular stomatitis virus glycoprotein G that affect membrane fusion activity. Role of the membrane anchoring and cytoplasmic domains in intracellular transport and localization of viral glycoproteins.
G enables mutant virus attachment and entry.