We are close, but not quite done. In words, the enzyme is partitioned between the unbound and substrate bound forms. We cannot at the outset expect to know how much enzyme is in each form. This gives the ratio of the rate of consumption of ES to its rate of production. Importantly, it is clear that M is not an equilibrium constant, because it involves the sum of rate constants from two different elementary processes.
Glucose oxidase catalyzes the oxidation of glucose by oxygen according to the following equation.
Thus by monitoring the absorbance at 725 nm, the kinetics of glucose oxidase catalyzed glucose oxidation can be readily studied. Construct a plot of initial rate versus glucose oxidase concentration. Construct a plot of initial rate versus glucose concentration. Click here for recipes for preparation of solutions. Safety Safety glasses must be worn at all times in the laboratory.