Equilibrating the resin and preparing a slurry 4. Resins come either dry or preswollen. If they are dry they need to be hydrated. The settled resin is therefore carefully decanted to discard these fines. The resin is then equilibrated in the buffer to be used for the analysis.
Equilibration usually involves pH'ing the resin, or buffer exchanges.
Finally, the slurry is degassed prior to packing the column. This will help minimize the formation of air bubbles. Packing the column Low pressure columns are typically packed using gravity. Add a small amount of buffer to the bottom of the column. Place a packing reservoir on the top of the column.