Transcription of exogenous templates as the zygotic genes is tigthly controled and no transcription is detected prior to the normal time for zygotic induction. Using viral promoters, we found that the presence of an activator capable to find its target within a chromatin context is sufficient to obtain a precocious transcription. We are also making use of this system to analyse the role during development of putative chromatin assembly factors. By studying mechanism underlying the control of chromatin dynamics, we hope to better approach the importance of chromatin changes in setting up a gene in an active conformation, as well as its maintenance. Both aspects are critical during development and highly compromised during DNA repair and in tumorigenesis.
Dual incision assays for nucleotide excision repair using DNA with a lesion at a specific site.
Recruitment of phosphorylated chromatin assembly factor 1 to chromatin following UV irradiation of human cells J. Cell. Assays for chromatin remodelling during DNA repair Dans Chromatin , Meth. Duplication and maintenance of heterochromatin at late replication foci J. Cell. Dans Chromatin Protocols , Meth.