The staining technique uses red carbolfuchsin primary stain that is able to enter the waxy coat of this genus. An acid alcohol is used during the staining, along with a methylene blue counterstain. Certain genera of gram positive bacteria are noted to form endospores. Review Questions List two types of microscopes that do not utilize stains for viewing cells. The gram stain is an example of a differential stain.
Counterstains involve using a stain of a different color than the primary stain.
Answers to Review Questions List two types of microscopes that do not utilize stains for viewing cells. Darkfield and Phase Contrast microscopes. The gram stain is an example of a differential stain. True Mycobacteria are identified using a stain known as the acid-fast stain. India ink is used in negative staining to identify a bacterial cell's capsule.