Record any results. Streak a sample from the lactose broth onto an EMB lactose plate using a sterile inoculating loop. Label the bottom of the plate with group identification and source of inoculum. Typical coliform colonies have a dark center or a metallic sheen. Record observations and indicate whether the water sample was confirmed to contain coliforms.
Flame sterilize the loop and allow it to cool.
Flame sterilize the loop and allow it to cool. Turn the plate in the same direction and spread the bacteria from the end of area B across area C. Flame sterilize the loop and allow it to cool. Flame sterilize the loop before setting it down.