Remove the syringe and discard the pre-filter. Elution of DNA from Column Load the syringe with 0.4 ml high salt buffer and reattach to the column. Elute the DNA from the minicolumn by passing the high salt buffer through the column into a 1.5 ml microfuge tube. A second elution is optional. You are highly recommended to use Suprapurereg.
Impurities are lethal to single-cell mouse embryos.
Determine concentration of DNA via optical density.