The pH of the corneal stroma can be measured utilizing the pH sensitive properties of the fluorescent dye fluorescein.
Fluorescein is instilled into the cornea by drops or iontophoresis.
The fluorescence of fluorescein in the cornea is measured by a modified slit-lamp fluorimeter. The ratio of fluorescence emission is proportional to pH and unaffected by the concentration of dye over a wide range. Stromal acidosis affects corneal hydration control. Implications and new directions for contact lens research. Stromal acidosis modulates corneal swelling.