Weigert myelin stain for axons Cortical layers Different functional areas show different pattern of layering and cell types. Shows Brodmann areas superimposed on functional visual areas. They don't line up too well. But this could reflect problems in Brodmann s classification. Afterall, he did that work almost 100 years ago.
Cytochrome oxidase is an enzyme used in metabolism.
In recent years, have observed particular patterning in visual cortex when stain for cytochrome oxidase. Sure enough cytochrome oxidase shows clear differences between functional visual areas. Supports hypothesis that functional areas correspond to differences in cytoarchitecture. Some connections easily visible with naked eye, e.g., visual pathway from eye to V1 Association bundles Each of the major white matter bundles is called a fasciculus. Then sacrifice animal and stain for the tracer.