Contrary to comparable software the algorithm identifies even complex repeats. Primers fitting to existing primers or completely new primer pairs can be created, and multiple constrains for primer design and exclusion criteria can be varied. The program makes appropriate suggestions for primer design constrains and primers calculated are given as a table listing according to conformity with the conditions. This program enables individual tailoring according to selected constrains, with maximum data mobility. It can also be used to detect repeats and unique sequences.
Inappropriate primers cause low yield, artifacts equivocal results and thus, misinterpretation.
They are generally the result of short DNA sequence repeats. An ideal primer should only bind to a unique sequence. To ensure this, the given sequence must be compared with itself to identify repeats. Individual programs only feature some functions and are unsuitable for data transfer. Methods A new program was tailored to meet these conditions.