But now i have a problem that i don't now how to prepare my own gel. The broad range markers generally have few proteins in that range, plus it seems a waste to load a 3-10 marker to see stuff between 4 and 6. I think, commercial IEF gels have a more accurate and reproducible gradient. Samples - max. 50 mM salt.
Filters are the anode and the cathode, respectivily.