Formation of a ligand-binding site for the acetylcholine receptor in vitro. The inhibitor was used in both in vitro transcription and in vitro translation reactions to protect the in vitro transcript. The inhibitor was used to protect 32 P-labeled RNA probes during RNA gel shift assays. The inhibitor was used to protect transcripts from an in vitro transcription reaction and the same transcripts during RNA gel shift assays. The inhibitor was used to protect RNA during the T4 RNA ligase step for 5' RACE analysis.
Human platelets display high-affinity receptors for thrombopoietin.
The inhibitor was used in a very innovative fashion. Peripheral mononuclear cells isolated from blood were directly lysed in a hypotonic buffer containing the inhibitor. Endotoxin induces rat lung ribonuclease activity. We show that within one hour of injection of endotoxin into adult rats, there is an induction of ribonuclease activity in the lung. We suggest that induction by endotoxin of ribonuclease activity is a novel mechanism by which cells could alter gene expression to meet an environmental challenge and caution that the presence of ribonuclease can hinder molecular biological analyses of tissue extracts from endotoxin-treated rats.