Preparation - we have preparation protocols available to help obtain the best sequencing results. Protocols will soon be available over the web . Dideoxy sequencing reactions are performed with fluorescent labels instead of the usual radioactive labels. With dye primer labeling, extension products are identified by using primers tagged with four different florescent dyes in four separate base-specific reactions. The products from these four reactions are combined and loaded into a single gel lane.
Labeled primers are available for common priming sites.
Thus, the growing chain is simultaneously terminated and labeled with the dye that corresponds to that base. An unlabeled primer may be used. Four sequencing reactions performed in one tube. Ordering Order forms may be obtained here in either PDF or HTML format, or may be picked up at 322 Hochstetter Hall. Completed forms should be faxed to, mailed to, or dropped off at, the facility.