The obtained data led to ring tests, and next year to an international co-operation with further laboratories. Perspektiven zum Ersatz des Belastungsversuches bei Rauschbrandvakzinen. Antigene des C.chauvoei. Possibilities and Limits of In Vitro Assays for Quality Control of Blackleg Vaccines. Quality Control of C. septicum Vaccines In analogy to the challenge test for the quality control of blackleg vaccines, vaccines of C.septicum have to be tested.
International regulations demand tests in mice and rabbits.
Rabbits are to be vaccinated and the detection of antibodies is to be done in form of a neutralisation test in mice. An in vitro method based on the lethal a-toxin will be established and tested as an alternative method for the animal procedure. A locality-specific vaccine was produced with a strain of C.septicum from an outbreak in cows on a German farm. The follow-up is monitored clinically by the local veterinary surgeon, serum parameters at the Institute. The efficiency of the locality-specific vaccine in comparison to commercial vaccines will be tested.