Quench on ice. Heat to 65 C for 10min.
Heat inactivate the enzyme when complete.
The volume should equal 25ul per time point. Heat to 30 for 5 min to equibrate at incubation temp. Dispense 20ul 10X mung bean buffer, 155ul DDW into eppendeorf tubes for each time point. Have on hand crushed dry ice. Add 20U of ExoIII per ug of DNAand vortex.