A wide range of concepts and techniques used in the modern laboratory are included in the exercises. A laboratory designed to provide the student with firsthand knowledge of the spirit and practice of current research in biochemistry and molecular biology. A long-term project, lasting the entire quarter, is carried out, in which students isolate restriction endonucleases and use them to obtain physical maps of DNA molecules. 105L. Genetics Laboratory Introduction to theoretical and practical aspects of genetic analysis in selected organisms presenting experiments on mutagenesis, recombination, and genetic mapping. The powerful genetic and molecular techniques available for yeast combined with the complete genomic DNA sequence offers opportunity for discovery of fundamental aspects of eukaryotic life.
Lab provides practical experience in using yeast as an experimental system.
Cell Biology Covers the structure, organization, and function of eukaryotic cells. Topics include biological membranes, organelles, protein and vesicular trafficking, cellular interactions, the cytoskeleton and signal transduction. Eukaryotic Molecular Biology Covers eukaryotic gene and genome organization, DNA, RNA, and protein synthesis, regulation of gene expression, chromosome structure and organization, and the application of recombinant DNA technology to the study of these topics. A laboratory designed to provide students with direct training in basic molecular techniques. Each laboratory is a separate module which together build to allow cloning, isolation, and identification of a nucleic acid sequence from scratch.