It's important for you to know when NOT to expect hearty laughter. It would be a waste of time to use your best material at a time when laughter normally wouldn't be expected. The poor response also brings your energy level down. Many consider brunch and lunch to be the best times of day to expect a responsive audience. In the afternoon people are starting to get tired so don't expect laughter to be as intense.
All female audiences tend to laugh more easily and louder than all male audiences.
Audiences that consist of more than 50 percent women are good too. No, I'm not talking about how much you weigh today. I'm saying that the size of your audience has a direct effect on the types of humor which are most appropriate. Members of small business groups tend to be too self-conscious to laugh much. Use short one-liners.