Chromatogram files from automated sequencers are directly processed. Base-calling and correction, consensus construction, and correction of consensus is possible with display of chromatogram curves. The GeneBase sequence gel reading module provides an algorithm for automatic lane-finding and sequence reading from gels scanned into TIFF files. Visual interpretation is made easy by simultaneous display of densitometric curves and the corresponding zoomed gel area.
Manual correction is possible on the densitometric curves or directly on the zoomed gel image.
Databasing and sequence editing GeneBase is a versatile multi-user system, in which each user defines own databases and customizes all settings. Each sequence is described by up to four custom information fields which are used by the database search algorithms. Lists of sequences, e.g. for multiple alignments, clustering or consensus determination, may be saved to disk. Formats from important databases such as EMBL and GenBank can be directly read. Sequence editing tools include a variety of useful features, such as voice reading, gel reconstruction, subsequence and restriction enzyme search, search for likely subsequences with adjustable stringency, invert, complement and translate functions, etc.