The mail message has two mandatory lines, three optional lines and a line identifying the query sequence as descibed below. No Specify 'tfasta' to compare a protein sequence against a nucleic acid sequence database translating in all 6 or 3 frames. If omitted, 'fasta' is assumed. This line identifies the Ktup value which specifies the sensitivity of the search. Values range between 3 and 6 for nucleic acid searches and between 1 and 2 for protein searches.
Lower values specify more sensitive searches but require more time to complete.
The default value for nucleic acids is 6 and 2 for proteins. No Options may be specified as described in the attached manual page. This line must be included in the message. It should be the last parameter and followed on the next line with the query sequence. See below for the format of the query sequence.