Introduction to many state of the art methods in molecular and cell biology, including isolation of DNA, restriction enzyme analysis, cloning of isolated DNA by ligation into vectors, transformation and selection of recombinant plasmids in E. coli , DNA sequencing, and computer- assisted analysis of sequences. Laboratories meet twice a week for the first half of the term. Special registration procedures apply. Students must consult the instructor by Tuesday, September 12, 2000. Continuation of MCDB 312La to more advanced methods and techniques in molecular and cell biology, including isolation of mRNA, synthesis of cDNA, RNA Northern blots for analysis of gene expression, making cDNA libraries, cloning of genes by screening of cDNA libraries.
Laboratories meet twice a week for the second half of the term.
Special registration procedures apply . Students must consult the instructor by Tuesday, September 12, 2000.