I'm only rude towards the weird crowd from the Shankar foundation. These people have been posting advice of dubious quality, and one have even been caught inventing bogus chapter from a molecular biology manual about a technique that doesn't exist. I wonder what else one may discover that's bogus. If you wish mediocre and misleading advice from people who know very little, you are welcome to it. I won't post anymore as I'm sure that makes you lot happier.
Ignorance is bliss.
I'm do a ligation over night with 400ng of 25bp fragments.