This is very useful for mixing and matching DNA from different species to combine together. Figure 15.3 2. Can move into bacteria 3. Replicate independently of bacterial chromosome, so they can be duplicated before division, ensuring that both daughter cells inherit a copy. After cutting human DNA and plasmid with the same restriction enzymes, you create the sticky ends that are attracted to each other by base pairing.
Ligation is the process of permanently attaching the two pieces.
D. Transformation 1. Bacteria are capable of taking up DNA from their surroundings by this process. How Dolly was cloned 1. In 1997 by researcher Ian Wilmut and colleagues at PPL Therapeutics. A cell was taken from udder of adult sheep and grown in culture in a laboratory to create many daughter cells.