Capillary bleeding may result from over manipulation and patients should be warned of possible spotting. When the samples are obtained they should be transferred to the slide immediately. The direct cervical scraping from a spatula should be spread with a single stroke using moderate pressure to thin out clumps of cells and mucus. Microscopic screening is much easier and less tiring to the cytotechnologist. If there is to be any delay between the sampling of the ecto and endocervix, the first sample should be spread and sprayed with fixative, being careful to protect the unused portion of the slide.
Immediate fixation is essential to retain optimal cellular detail.
Generally, most spray fixatives have a dual action in that they fix the cells and when dry provide a thin protective coating over the smear. The container should be held 15 to 25 cm from the slide to prevent dispersal or destruction of the cells by the force of the spray. Alcohol fixatives may also be used but are not as practical. This method of fixation provides excellent results and may be used for all smears prepared at the side of the patient. Sufficient drying time is necessary to avoid the sample sticking to the slide holder.