Hence, it is of interest to study the modification of the RPA subunits by analogs containing other reactive groups. In this case, it is of interest to attach another reactive group and study the efficiency of RPA subunit labeling depending on the length of the linker connecting this group with the base. The data indicate that each analog labels DNA-polymerase beta to a similar extent. These interactions influence the function of RPA in replication origin and during elongation. This interaction of RPA with DNA-polymerase beta is not very tight and thus cannot be demonstrated by other methods.
Chemical modification enables demonstration of weak interactions between biopolymers.
Thus, changes in linker length and structure of the reactive group have no effect on the pattern of modification of RPA subunits. The p14 subunit was not labeled, whereas the p32 and p70 subunits were labeled by all analogs. Thus, the set of dTTP analogs containing an arylazide group attached to the base by a linker with variable length can be fruitful to study the interaction of DNA with DNA-binding proteins. All rights reserved.