FAD binding is inhibited by several nucleotides. Glucose oxidase is assayed using a method which measures reaction velocity by determining the increase in absorbance at 460 nm resulting from the oxidation of o-dianisidine in a peroxidase coupled system.
Glucose oxidase has application in oxidation reactions.
Glucose oxidase is also used clinically and analytically in the determination of glucose. For analytical applications a purified glucose oxidase should be used since it is low in other polysaccharidases. Subunit Structure of Glucose Oxidase from Aspergillus niger . Studies on the Molecular Complex of Flavins. Activity and FAD-Fluorescence Change Caused by the Chemical Modification of Tryptophyl and Tyrosyl Residues in Glucose Oxidase.