Size and shape of the particles 4. Properties of the media 5. Most commonly used media 3. At a pH of 8.6, protein usually carry a neg. Albumin, alpha 1, alpha 2, beta, gamma.
Albumin migrates the farest, and gamma the least.
Produced by the Liver, so it is decreased in most liver diseases including Liver Cirrhosis. Migrate in the alpha 2 region,It combine free hemoglobin via the alpha chain. If a flipped pattern seen it indicates MI Hemoglobin Electrophoresis Alk. Be careful not to overload the sample otherwise a distorted zone will be seen. Bisalbuminemia is seen when therapeutic drug is present in the serum.