These variations include surface scattering, fiber structures, mechanical manipulation damage, and inherent sample variations. Therefore, this study was preformed to look for general trends, as common in biological studies, and not for absolute numbers 10 . At 1400 nm the beam is highly scattered. Spatial scattering seems to decrease with increasing wavelength. The moving images are much smoother than the stationary images.
All images have a significant pedestal and spot distortion.
Figure 3 . Minimum spot sizes obtained after focusing through sclera. Normalized images on the back surface tissue are shown. Scattering appears to reach a minimum at 1600 nm. The upper left hand corner inset is the minimum unscattered spot obtained without the tissue.