Change gloves before working with bind silane and take care not to cross contaminate the plates with the two treatments. The acrylease treatment only needs to be repeated every four gels or so. Apply with a kimwipe and coat the inner side of the larger plate with one ml and allow to dry 4-5 minutes. Wipe the plate with ethanol in one direction and then perpendicular to first direction, don't use too much pressure. This treatment needs to be repeated every time.
Acrylamide is toxic.
Wear gloves when handling solution and face mask when weighing out powder. A safer alternative is to buy a premix. Rinse all glassware and plastic ware with d.i. Warm and stir the mixture in a beaker of warm water until all the urea is dissolved. Add 1.25 g of amberlite resin and stir 5 min.