Disposable gloves are not reused. All manipulations of infectious materials, necropsy of infected animals, harvesting of tissues or fluids from infected animals or embroyonate eggs, etc., are conducted in a Class II or Class III biological safety cabinet. Respiratory and face protection is used when in rooms containing infected animals. The laboratory is separated from areas that are open to unrestricted traffic flow within the building, and access to the laboratory is restricted. Passage through a series of two self-closing doors is the basic requirement for entry into the laboratory from access corridors.
Doors are lockable.
A clothes change room may be included in the passageway. Each laboratory room contains a sink for handwashing. The sink is hands-free or automatically operated and is located near the room exit door. The interior surfaces of walls, floors, and ceilings of areas where BSL-3 agents are handled are constructed for easy cleaning and decontamination. Seams, if present, must be sealed.