Do not vortex. Run the sample on the cytometer within 3 hours after addition of Solution C. Figure 1. Histogram of stained DNA nuclei from paraffin-embedded tissue. Spermine tetrahydrochloride is irritating to the skin and mucus membranes. Propidium iodide is a suspected mutagen.
Fresh tissue is preferable to paraffin-embedded material for most purposes.
This technique for extracting and staining nuclei from paraffin-embedded tissue is intended for use only in retrospective studies. Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. An indicates this product is an analyte specific reagent. Available in the U.S. only. An indicates this product is for in vitro diagnostic use.