Add 20 mL glacial acetic acid. Stir until solution contains no undissolved particles. Prepare immediately prior to use. Do not store this solution. Reagent contains diaminobenzidine.
Exposure can cause cancer.
If skin contact occurs, flush with water. To 900 mL of distilled water, add 100 mL of 10X Stock Solution. Final pH should be 7.2 0.2. Do not attempt to modify pH with acid or base. Do not add sodium azide.