Being the daughters of the Tsar, the girls lead a much cloistered life. Olga, Tatiana, Marie, and Anastasia. They used the acronym for many things such as signing letters and birthday cards and even with their parents when forming a united front. As one learns about the Imperial family, a sense of deep sadness consumes the spirit as the tragedy of their deaths unfold. Speculation and time have clouded the events that night in the cellar.
Chances are very good, that the entire family perished.
It is possible that Anastasia and Alexei escaped, but it is not probable. The family, who loved each other so deeply and passionately, perished together. No one member was left behind to long for and grieve for the rest. True, not all died instantly, but the decades since the murders have not eaten away at a single soul so as to contaminate their spirit with hatred, or worse indifference towards their country.