Rinse with water. Rinse top of gel well. Insert comb teeth down until teeth just touch the gel. Mount the plates in apparatus. Fill chambers with 0.5X TBE and prerun at low wattage for 10 min.
Load oligonucleotides to gel.
Decant buffer from apparatus. Remove gel and lay flat. Remove comb and side spacers. Pry apart plates with a spatula. If the gel is stuck to the top plate, resettle plates and invert.