The antibody is purified by Protein A affinity chromatography. Rabbit Anti- Human EPO is provided lyophilized from phosphate buffered saline, to which no preservatives have been added. Description Erythropoietin is a humoral factor that stimulates erythropoiesis. 1 EPO has four introns and five exons and contains 165 amino acids. 2 EPO induces growth and differentiation of burst forming units and colony forming units into mature red blood cells.
Human erythropoietin stimulates growth of rodent bone marrow cells.
2 Recombinant erythropoietin is produced in CHO cells by a DNA sequence encoding the human EPO protein. The ND 50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of rhEPO, when rhEPO is present at a concentration just high enough to elicit a maximum response. If aseptic technique is used, no further filtration should be needed for use in cell culture environments. Avoid repeated freezing and thawing. References Carnot, P., et al., C.R. Acad.