Good data can be obtained with methods such as Millipore Ultrafree-MC filters, Dynabeads, Centricon and Wizard PCR preps. For best results, primers must be designed carefully, avoiding particurarly the potential to form primer-dimers and secondary structures. Results DNA template is sequenced by using the provided primer with fluorescent automatic DNA sequencer. Sequencing reactions are performed with fluorescent dye terminators following thermal cycling protocols with a Perkin Elmer Thermal Cycler. Responsibility Submitters bear responsability for providing samples capable of yielding good sequence.
Every run includes control samples to check both sequencing reactions and gel running performances.
The cost of the run submitted will be applied even if the sample fail to produce useful sequence data, unless control samples also fails. All rights reserved.