Protein dephosphorylation, in combination with protein phosphorylation, is the crucial mechanism in regulating multiple cell functions including signal transductions in immune system and cancer. Preparation of rat hepatocytes. The rat was anesthetized with nembutal and the liver was perfused by injecting a medium containing collagenase from the portal vein. Isolated hepatocytes are recovered from the perfused liver and cultured under various conditions to investigate roles of protein phosphatases in proliferation of liver cell. Apoptosis of lymphocytes induced by a tautomycin derivative.
Arrows indicate the position of the hybridization signals.
Purification of proteins by chromatography on FPLC system. Extracts of tissues and cells are loaded on this system and the constituent proteins are separated by chromatography according to desired schedules inputted in advance to the controller. Various enzymes, their substrates and regulatory proteins have been purified on this system.