Histamine is extracted from a sample in a quick water extraction process. This extract is filtered and then diluted into a buffer solution supplied with the test. The buffered sample is then mixed with enzyme-labeled histamine conjugate. The mixed solution is then transferred to an antibody coated well, where the sample histamine competes with the histamine conjugate for antibody binding. Substances that are not antibody bound are removed in a washing step.
Enzyme substrate is added, which turns blue due to the action of bound conjugate.
Red stopping solution is then added and the color of the resulting solution is observed visually. A control is provided in the kit and must be assayed with the samples. If a sample well is more blue than the control, the sample contains less histamine than the control. No instrumentation required. Provides distinct visual color results.