The antifreeze protein keeps the cell from freezing. It does that by attaching to the ice and changing its geometry. The molecule has a salt bridge, A dipole moment and many amino acid. The protein shape is mostly a helix. The influence in these parameters on the energy.
Energy changes in the conformation of the ice.
Its exact structure has been hard to investigate. Drug design based on this protein and its difficulties. Binding of NAD to DT and its consequences. It has been suggested that this enzyme helps dispose of bacteria after they have been killed by other means. M is well known for its lytical activity on cell membranes.