PCR amplification of very low amounts of genomic DNA using a degenerate primer. Amount of PCR product decreases with the decreasing amount of template. Multiplex PCR using primer mixture A in 1x PCR buffer. As the amount of template drops, most products become gradually weaker. Cycling conditions were identical.
Arrow indicates the presence of an unspecific product.
As the DNA template decreases, some bands become weaker in the multiplex reaction. Over the same range of concentrations, this effect is not so visible when only one primer pair is used. Again, the amount of PCR product decreases with the reduction in template DNA but less so when only one primer pair is used. As in Fig. 30 above, to amplify such reduced amounts of DNA template, the same program with low annealing temperature had to be used. Arrow indicates that the polymorphism at locus 4 is detected with the decrease in DNA template amount.