We do not discourage samples prepared by any particular methods. For best results, primers must be designed carefully, avoiding particurarly the potential to form primer-dimers and secondary structures. The DNA template is sequenced by using the provided primer with a fluorescence automated DNA sequencer. Sequencing reactions are performed with fluorescent dye terminators following thermal cycling protocols with a Perkin Elmer Thermal Cycler. Responsability Submitters bear responsability for providing samples capable of yielding good sequence.
Every run includes control samples to check both sequencing reactions and gel running performance.
The cost of the run submitted will be applied even if the sample fails to produce useful sequence data, unless control samples also fail. The turn-around time is 1 week. All rights reserved.