PC was supported by an EMBO long term fellowship, RB was supported by the Pew Scholar's program, Hoechst AG, and an American Cancer Society Faculty Research Award. Tables and Figure Legends Table 1. Rate and equilibrium binding constants of 6 aptamers. Library plasmids from these colonies contained 14 different sequences. In vitro interaction between peptide aptamers and Gst-Cdk2.
Figure shows portions of the curve used to calculate rate binding constants.
Association phase starts upon aptamer injection and dissociation phase upon running buffer injection. We used 5 microliter of 100-fold diluted extract in 30 microliter reactions.