An overview of physical approaches for examining the detailed structure and dynamics of biological molecules. First semester concentrates on the thermodynamics and kinetics of biochemical interactions, with a view towards describing phenomena such as ligand binding, cooperativity, conformational changes of proteins and nucleic acids, and biochemical regulation. In the second semester the major emphasis is on spectroscopy, including optical as well as magnetic resonance methods. X-ray, electron, and neutron diffraction methods will also be introduced, as will direct imaging techniques such as electron and scanning tunneling microscopy. A consideration of the physicochemical principles and technical approaches current in the study of biopolymers.
Emphasis is placed on thermodynamics and statistical mechanics.
Multiple equilibria including subunit interactions, ligand binding, structure and function are discussed in detail. A thorough study of nuclear magnetic resonance principles and practices. Attendance is required of all graduate students in biochemistry. A continuing seminar based on papers in the current literature. Required participation by departmental staff, fellows, and graduate students.