Be sure bolts and entire rosettes are submerged. Bacterial solution can be extended by reusing for at least one additional pot. Place beaker into bell jar. Draw a vacuum until bubbles form on leaf and stem surface and solution starts to bubble a bit, then release vacuum very rapidly. Practice on a few dispensable plants first.
Good infiltration is visibly apparent as uniformly darkened, water-soaked tissue.
Be sure to have good traps in your vacuum system or you will quickly saturate the pump oil. Remove plants from beaker, lay them on their side into a plastic flat and cover with plastic wrap or a dome to maintain humidity. The next day, uncover plants and set upright. Grow approximately four weeks, keeping bolts from each pot together and separated from neighboring pots. Pour selection plates.