Excess solution is aspirated. The solution is allowed to sit for 5 minutes before aspiration. The slide is set on wet paper towels in a sealed chamber and incubated at 4 o C overnight. Excess solution is aspirated. The slide is set on wet paper towels in a sealed chamber and incubated at room temperature for 2 hours.
Excess solution is aspirated.
A coverslip is placed and sealed with nail polish. Slides are stored at -20 o C until examination.