The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1. The suspension is incubated at 37 o C without shaking and checked periodically. Excess solution is aspirated. The solution is allowed to sit for 5 minutes before aspiration. The slide is set on wet paper towels in a sealed chamber and incubated at 4 o C overnight.
Excess solution is aspirated.
The slide is set on wet paper towels in a sealed chamber and incubated at room temperature for 2 hours. Excess solution is aspirated. A coverslip is placed and sealed with nail polish. Slides are stored at -20 o C until examination.