The general objective of the project is to begin to build the proteome of membrane neuronal proteins, using mammalian models. This will imply the development of dedicated techniques for the analysis of membrane proteins, as they are quite reluctant to standard proteomics techniques. This development will be sustained by the permanent staff of the group, which is deeply involved in this topic. Skills are therefore required in cell culture, subcellular fractionation, 2D electrophoresis and immunoblotting. Fluent english or french is required, as well as a PhD in biochemistry or cell biology.
All nationalities are welcome.
The laboratory is near the Paris-Sud University Campus, about 20 km south of Paris. Ten partners are involved and will characterise 5 key developmental stages of considerable interest for academic laboratories and plant breeding companies. The first part of the work will be concerned with developmental analysis and identification of responsive proteins, together with search for correspondence between identified proteins and ESTs obtained in other laboratories involved in the program. For mass spectrometry analysis, the work will be done in collaboration with Dr. M. Zivy and L. Negroni. Participation to coordination meetings and presentation of the results will be part of the position.