Re-attach the vacuum hose to the desicator. Start the pump and rotate the desicator lid to open. Check that the pump exhaust is not pointed into the room to avoid blowing the chloroform into the lab. Disconnect the hose from the lid and allow air to flow into the desicator. Repeat flushing 8 times.
Incubation Carefully remove the lid from the desicator.
Cap both bottles. Place the bottles into the 25 o C environmental chamber for 10 days. Stuff needed Alcohol-free chloroform Place 300 ml of chloroform into a beaker. Heat carefully to 65 o C or until just below boiling. Boil for 5 to 10 minutes.