This is an example rendering of Wayne Rasband's mri-stack.tif to check whether VolumeJ is functioning properly.
All parameters are default except the ones in bold.
Look right between the two images into infinity and you may see the stereo effect. Depth coded rendering of z-series of a HeLa cell, transiently transfected with mitochondrially targeted DsRed1. The z-series was obtained with a Biorad MRC1024 LSCM and deconvoled using a 3D blind deconvolution package from Noran. The rendering was depth coded to demonstrate that there are mitochondria above and below the nucleus rather than in it, as they appear to be in a regular surface rendered image. Image courtesy of Tony Collins.