Oligonucleotide lengths are listed above each sample. A single peak corresponding to the theoretical mass denotes a successful synthesis. Often the observed mass is 18 Da lower than the theoretical mass, presumably due to a dehydration, the source of which is not known. This small mass difference is easily distinguished from the much larger mass differences resulting from a failure in synthesis of the oligonucleotide. Standard Operating Procedure 1.
Group oligonucleotides in ascending order by length, along with appropriate markers.
Markers are oligonucleotides of varying lengths and base compositions synthesized by our resource laboratory. Oligonucleotides less than 15 bases in length will not resolve on these gels and should be submitted for mass spectrometry analysis, if available. Resuspend the sample in 6 mL of sample buffer and vortex. Heat the sample at 100 o C for 2 minutes. Chill the sample on ice.