Since the early part of this century, differences in tumor metabolism compared to non-tumor cells have been reported and include increased glycolysis, lactogenesis, cholesterolgenesis, and changes in fatty acid composition. To examine these questions, I use stable and radio isotopes to quantitate metabolic events coupled with in vitro and in vivo models. I use the MCF-10A cell line transfected with the Harvey ras oncogene to examine the effect of oncogene expression on cellular metabolism. I use a transplantable tumor line implanted on the femoral artery and vein to examine the interaction between host, tumor, and diet. This isolation allows separate, yet simultaneous, measurement of tumor and host metabolism.
Glutamine appearance is increased in precachectic tumor-bearing rats.
Lipolytic suppression following carbohydrate ingestion limits fat oxidation during exercise. J. Physiol. 273, E768-E775. Metabolic effects of triiodothyronine replacement during fasting in obese subjects. Adipose tissue and the effects of fat and calories on breast tumorigenesis in rats. In vivo study of the biosynthesis of long-chain fatty acids using deuterated water.