Real time chemical images of serotonin and protein released from individual cells are obtained. The images show that the amount of material released and the time delay of the event varied from cell to cell, and that different regions of a cell behave asynchronously in releasing material. Similar studies of the release of insulin from rat pancreatic b cells and of adrenaline from bovine adrenal medullary cells will also be presented. We have also developed a novel visualization scheme for studying cell signaling. New insights into neuronal signal transduction pathways are obtained.
Electrophoresis is normally a statistical process involving many molecules of a given type.
The migration velocity is used for sizing DNA and proteins or for distinguishing molecules based on charge and hydrodynamic radius. We will describe a high-throughput imaging approach that allows determination of the individual electrophoretic mobilities of many molecules at a time. Travel east on Montgomery Village Avenue for approximately 1.5-2.0 miles, passing Lake Forest Mall and then a large lake on the right. The restaurant, a white house, is on the left in a strip mall shortly after the lake. Spectral Dimensions, Inc . designs and manufactures chemical imaging instrumentation.