When taking plant material to grow on using plant tissue culture it is important to get the most appropriate material for the end product you are aiming for. Plant tissue has been shown to be totipotent,but different tissues will require different treatment to produce whole plants if that is the aim. Tissue that is dividing such as at the nodes and leaf axils,leaf peiole material are often used. Sharp cuts will decrease the amount of decaying material present,and decrease the possibility of infection. The use of a sharp scalpel is advised.
Forceps are necessary to move the plant material to the growing media.
The cutting should take place in a sterile environment and the growing media only exposed when the plant material is placed in it,after which it should be sealed. Forceps Scalpel Disinfectants-for surfaces,implements and plant material. Laminar Flow Cabinet Bunsen burner Containers-petri dishes,small clear plastic containers,glass jars. The source of the plant material is important as some plant tissue is better suited to tissue culture than others,the ability of plant material to grow and divide in vitro is known as totipotency,but different plant material will need different control to form new plant material. The plant material may form a new embryo,callous tissue or a whole plant depending on how it is looked after.