Chill for an hour in -20degreesC freezer, then spin 30 min. If pellet is difficult to see, use a light source to watch the pellet while removing the liquid. Take up pellet in 200 l 0.1X TE or HPLC H20. Flick the tube with your finger to make sure the DNA is all in solution. You may wish to de-stain a gel for15 minutes in a water bath if the background fluorescence seems too bright.
Equivalent sucrose grinding buffers work fine.
For other recipes see Maniatis.