This will allow you to compare the analytical performance of these two methods of analyzing for iron. To do the AAS analysis, you will first prepare a series of calibration standards. The absorbance data for the standards will be used to generate a calibration plot, and the equation of the line will be used to determine the amount of iron in the vitamin. Section 22-1 in Harris gives an overview of atomic absorption spectroscopy. Dilute solutions of HCl and HNO 3 are used in this experiment.
Glasses are adequate eye protection.
All solutions from this lab may be poured down the sink. After disposing of acidic solutions, flush the sink with plenty of water. The solutions used in AAS analysis are quite dilute. Pipets should be thoroughly cleaned first, then conditioned with the solution to be delivered. Never put a pipet or anything else into the stock bottle, and pour no more from the stock bottle than you need.