2 hrs remove medium and collect into a waste bottle.
Cytotoxic waste needs to be kept separate for incineration.
Return to hood, hit flask on the end to dislodge cells. Add an equal volume of medium, transfer cells to a 10 ml tube. Take a sample to count. Feeder plates can be used 2 hrs after seeding, and for up to one week later. Store at 4 C wrapped in foil as mitomycin C is light sensitive.