Into the other series of solutions place a drop of methylene blue solution and mix. Allow 30 minutes for the tissues to equilibrate with their solutions, then decant to solutions containing the plant material into the corresponding empty, but labeled test tubes. This allows you to test density without interference from the plant material. Extract a little of the methylene blue-dyed solution using a pasteur pipette, carefully lower the pipette tip into the corresponding solution in which the plant material had been placed, and release a drop to determine whether the drop rises, sinks, or remains at the same level. Join the 6 points by a line and estimate the water potential of the tissue as that solution water potential where the drop would not sink or rise.
Lab Reports are due at the beginning of the next lab session.
If you do not get the expected results, the interpretation should be a short explanation of what may have happened that altered the expected results.