The autoclave functions like a pressure of sorts to steralize the growth medium. This avoids the formation of precipitates which normally result when MgSO4 is autoclaved along with the other components in the growth medium. This is the standard recipe for growth medium. The compounds used to grow the medium can be all be ordered through scietific supply catalogues. We had all the compounds in our lab except yeast extract and bactotryptone.
Equipment is also a big part of the job.
When growing bioluminescent bacteria they need oxygen which can easily be provided when a water bath shakeris used to keep the cultures oxygenated. This piece of equipment can be hard to find. You may find it in larger labs in your city. Erlenmeyer Flasks can be found in most labs. These can be found in most labs.