The subcutaneous tissue is dissected and muscle fibres are seperated to visualise the vein. Two threads are passed below the vein. Two ends of each threads are approximated with artery forceps. The vascular structure is confirmed to be vein by Non-obliteration of the radial pulse on lifting the loops of thread containing the artery. Neural tissue is firm and cannot be cannulated.
Distal thread is tied to obliterate the lumen of the vein.
The distal thread is pulled down and nick is made on the wall. The lumen of the vein is dilated with the help of an artery forcep. The bevelled catheter or infant feeding tube is introduced in the vein through the nick and pushed inside. The pulsatile blood column indicates, position of the tip of the catheter. The proximal thread over the vein is tied around the catheter.