PCR allows us to target specific regions of DNA which have been proven to be unique to the organism we are interested in and create millions of copies of these gene fragments. This allows us to produce enough DNA to see on an agarose gel. The exquisite sensitivity of PCR is it s own downfall in many environments. To avoid the problems of contamination leading to false positive results- we have designed the laboratory so that DNA extraction, PCR reaction preparation and analysis of the products are done in separate, environmentally controlled rooms.
Extreme care is taken to avoid cross-contamination of samples.
In addition the laboratory has been designed bearing in mind that these micro-organisms can constitute a hazard to human health. Class II microbiological safety cabinet used for the initial stages of DNA isolation from blood and tissue samples. Using the scientific literature we have identified DNA sequences unique to each of the organisms being studied and have established methods for amplifying these regions of DNA selectively and reproducibly. Agarose gel electrophoresis and imaging room where the analysis of the PCR reactions takes place.