The RNA polymerase eventually falls of the DNA at an ill-defined location. The long RNA transcript then gets cleaved at a specific location near the 3' end to produce the discrete sized pre-mRNA. 11. Explain why polycistronic eukaryotic mRNAs can not work. Once the ribosome has bound to the cap it will start protein synthesis at the nearby AUG codon. Translation from a downstream coding region, therefore, is not possible because the ribosome does not have any cap structure to use for starting translation.
Enhancers play an important role in eukaryotic gene regulation.
3 points It is believed the TFs which are bound to the enhancer can interact directly with TFs bound to the promoter by allowing the DNA to bend and loop out between these two locations. The figure at the right shows the results of an experiment you perform to determine the location of a promoter. The horizontal line indicates a gene, whose transcript starts at position 1. A series of constructs containing different deletions are created using genetic engineering techniques. You put each of these DNAs into a cell and determine if transcription occurs from this gene.