Dry Transfer Dry Transfer Trans-Bot SD Assembly 1. Prepare the transfer buffer. Following electrophoresis, equilibrate the gels in transfer buffer.
Equilibration facilitates the removal of electrophoresis buffer salts and detergents.
The length of time required for equilibration is dependent on the gel thickness. Cut the membrane to the dimensions of the gel. Cut filter paper to the dimensions of the gel. Completely saturate the filter paper by soaking in transfer buffer. If more than one full-size gel is to be transferred at one time, cut a piece of dialysis membrane with the appropriate molecular weight cutoff to the dimensions of the gel.