The bolt should enter at the same location as described for gunshot euthanasia. It is important to follow the manufacturer's recommendations on the selection of the cartridge strength appropriate for the size of the animal. Non-penetrating captive bolt guns will stun and not kill the animal. Poorly coordinated kicking or paddling movements and a period of relaxation will follow, and the pupils of the eyes should be fully dilated. It is important to confirm that the animal is dead y examining the vital signs of life.
Death is confirmed by the lack of rhythmic breathing, heartbeat, and corneal reflex.
An additional euthanasia procedure may be required if there is evidence of any vital signs.