Results have been published in The Immortalist. Because of the difficulty of obtaining here complete cryobiological literature since 1986, we are not sure whether our work is the first of its kind. Freezing of adult mammalian brain tissue with glycerol protection and use of registration BEA to indicate function. Freezing the cat heads to - 90 C did not result in integrative BEA, although single neurons had function. In cryobiology there is much better control of conditions and procedures, and often focus on just one or a few types of cells or tissues.
Cryobiology usually judges success by restoration of vital functions and spontaneous reanimation.
Revival after cryonics may need, and cryonics may allow, artificial or forced or guided reanimation, possibly with deep reconstruction of the whole biological system. I demonstrated and can show in detail that searching for new endocellular hydrophilic cryoprotectants is not promising. Glycerol, ethylene glycol, methanol, and many other compounds that lack specific toxicity at the cellular level may have even strong toxicity at the level of the organism. I think solution of this problem is far in the future, since it demands thorough understanding of the organism's biological organization. I repeat that further significant progress in cryobiology may take a long time.