Recrystallization of the product is no normally required but if desired DNPO can be recrystallised from nitrobenzene or ethyl acetate and TCPO - from benzene. The experiment is better performed in a darkened room. 10-15 ml portions of this solution are added into test tubes to which added a few mg of sensitizers. Reaction with TCPO also requires addition of ca . 0.2 g of sodium salicilate in each tube.
Chemiluminescence is initiated by adding ca .
Amongst the many colours produced, the yellow emission from rubrene is the most easily observed for larger audiences or in rooms that are not completely dark. The organic solvents are flammable, and care should be taken in their use and disposal. In particular, they should be stored in well-ventilated cabinets or hoods separate from acids and oxidising agents and isolated from sparks and flames. Organic solvents containing hydrogen peroxide should not be evaporated to dryness, as explosive organic peroxides may form. The fluorophors used in this demonstration are suspected carcinogens.