Amphibian blood contains nucleated erythrocytes, leukocytes and thrombocytes. Amphibian erythrocytes are biconvex, oval and the largest of all vertebrate erythrocytes. Accurate RBC counts are obtained by means of hemocytometer and standard counting procedures. The use of automated white cell counters is contraindicated. Anesthesia Delicate aquatic species such as Xenopus should be handled with soft nets for unanesthetized procedures.
Chemical restraint is required for prolonged or invasive procedures.
Anesthesia is judged by loss of righting reflexes and respiratory effort. At low temperatures cutaneous respiration appears to provide sufficient oxygen to support life. The absorption and excretion of anesthetic agents are directly affected by environmental temperatures. Therefore, amphibian anesthetic dosages are frequently listed with environmental temperatures. Once the animal has been immersed in the bath, sedation should occur within five minutes.