Injections are made in the lateral aspect of the lower left quadrants. Caution must be taken to avoid puncturing a distended urinary bladder, the bowel or the liver. The most frequently used sites are the back muscles lateral to the vertebrae and caudal to the Aab, or the lateral thigh musculature. Volume should not exceed 0.25 ml per site for adjuvant and antigen combinations. If repeated injections are to be made, rotate sites.
Adequate restraint is important.
The are most frequently used is between shoulder blades. Volume should not exceed 0.25 ml per site for adjuvant and antigen combinations. The most frequently used site is over the shaved back. Volumes should not exceed 0.05 ml per site for adjuvant and antigen combinations. Injection sites should be spaced 3-4 cm from each other to prevent confluence.