If necessary, make drawings to illustrate your point. In detail, describe how you would clone the rabbit globin gene into E. coli. 1. pBR322, a plasmid with the ampr and tetr genes that confer resistance to ampicillin and tetracycline, respectively. There is a BamH1 restriction in the tetr gene 2. BamH1 restriction endonuclease 3.
Bacterial colonies growing on a nutrient agar plate containing ampicillin are blue in color.
These bacterial colonies are the result of a recombinant DNA experiment using the pUC18 plasmid which contains the ampr gene and the lacZ gene. Explain the basis for your answer. There are many practical benefits of recombinant-DNA technology. However, initially many people were very afraid that cloning human genes could produce recombinant bacteria and viruses that could unleash deadly cancers or disease among the public. Discuss the two specific methods of containment that scientist have developed to prevent escape of recombinant organisms into the general population.