Use tested by Worthington using new-born rat pup spinal cord. The package contains sufficient materials for dissociation of five separate tissue aliquots of up to 0.3 - 0.4 cubic cm each. Store at 2-8deg. Store at 2-8deg. This material is 0.22 um membrane filtered and lyophilized in autoclaved vials.
Brief incubation is needed to insure full solubility and activation.
Store at 2-8deg. C, pH 6.2, after activation in a solution containing 1.1 mM EDTA, 0.067 mM mercaptoethanol and 5.5 mM cysteine-HCl for 30 minutes. D2 A component of the Worthington Papain Dissociation System. This material is 0.22 um membrane filtered and lyophilized in autoclaved vials. Avoid vigorous mixing.