For further information contact Christine Brakel. The resultant fragments can be easily sequenced and the data assembled with little or no sequencing redundancy. In addition to the human genome, all 3 billion base pairs of which are to be completely sequenced by the year 2005, a great number of other genome sequences are currently being determined.
Current techniques are time-consuming and wasteful.
Although the sequencing of DNA fragments of an appropriate size is more or less automated at this time, there are no readily automated schemes for preparing the clones for that automated process. This technology addresses these issues. The vectors useful for this process, the modifying enzymes and reagents for producing an ordered set of DNA fragments covering up to 20,000 to 30,000 bp are provided. The vectors and enzymes of this technology are further useful for the production of single stranded DNA probes. Because the vectors can be maintained in a single-copy state in the cells, the system can also be used to maintain clones of normally toxic DNA sequences that can be amplified for production purposes by induction.