Nitrosoguanidine tends to be the agent of choice. 60 and 90 min. You may need to spin the cells for 2 - 4 min to ensure a good pellet. A survival curve can be plotted by plating 100 and 1000 cells per plate and plotting percentage survivors as a function of exposure time to the mutagen.
Cells lose viability but keep for a week or so.
You may consider doing the survival curve after leaving the cells in the cold room overnight. NG is inactivated by soaking any contaminated materials in 0.1 M HCl overnight. Return to the index of Section 1 . All rights reserved. See Disclaimer statement.