Phospholipase C distribution in single smooth muscle cells is detected using immunofluorescence labeling and fluorescence microscopy. Cytosolic and membrane fractions are separated by ultracentrifugation of smooth muscle homogenate. Phospholipase C in the two fractions are separated from other proteins by SDS-polyacrylamide gel electrophoresis. Phospholipase C is detected using immunoblotting. These mechanical parameters are measured using a computer-based lever system and isometric force transducers.
Cytoskeletal reorganization is controlled using pharmacologic agents.
We hypothesize that passive immunosensitization modulates mechanosensitivity of airway smooth muscle. Passive immunosensitization is induced by treating airway smooth muscle with immune serum. An, Steven S. and Chi-Ming Hai. Mechanical strain modulates maximal phosphatidylinositol turnover in airway smooth muscle. Wong, Chun Tung and Chi-Ming Hai.