The mutation is in the RecA gene and renders RecA protein resistant to activation following UV irraditaion. The mutation is in the RecA promoter and results in the complete absence of RecA protein synthesis. The mutation is in the LexA gene and the mutant protein binds irreversibly to LexA operators. The UvrBC complex is an endonuclease. UvrA is a single-strand specific endonuclease.
Branch migration is an isoenergetic process that occurs spontaneously.
Holliday intermediates occur only during general recombination. The RecBCD enzyme is an exonuclease. The RecBCD enzyme has three subunits. The RecB and RecC genes are co-transcribed in E. coli. You get the following results.