I was hopeful of raising consciousness on Very High Pressure, but I would love to see more work on both High Pressure and Very High Pressure. Thanks to work by Ukrainian researchers, Robert Ettinger now feels confident that cracking is not a problem for liquid nitrogen cryostasis. Even if this is true, there remains the larger problems of freezing damage and loss of viability. I believe that immediate use of High Pressure would virtually guarantee reduction of freezing damage and increased viability. Greater use of electromicroscopy in connection with cryopreservation procedures will hopefully result in more appreciation of the distinctions between greater and lesser freezing damage.
Cryoprotectant toxicity is reduced at lower temperature.
Therefore, the use of High Pressure to maintain a liquid state at -20 degrees Celcius and below could mean introducing cryoprotectant at temperatures at which their toxicity is minimal. This could be of particular benefit for DMSO since it is far less toxic at low temperature and it perfuses cells so much better than glycerol. And, of course, arousing the interest of people who have resources needed to investigate these questions.