This technology has a variety of applications. An in-lane standard allows reliable detection of mobility differences. Microsatellite markers or STRs can be detected easily by PCR amplification, separation by electrophoresis using the 377 DNA Sequencer, and analysis with the Genescan Program. The PCR reactions can be multiplexed with up to 3 reactions in a single lane. Bovine paternity kits as well as human identity kits are available.
Allele frequency is also avaiable for horses as well.
Heterozygous or homozygous status can also be determined using Genescan. Southern blot analysis. The Genescan program takes advantage of the ability of a single nucleotide change to alter electrophoertic mobility. 150 samples can be screened within a 2 to 4 hour run after PCR amplification. After SSCP analysis, samples that have caused a mobility shift can then be sequenced to determine the nature of the mutation.