We have developed a microstamping method for depositing biomolecules from aqueous solution onto glassy substrates. The technique is additive, in comparison to the subtractive laser and photoresist based techniques. Unlike previous photoresist, laser, and stamping approaches, this technique is extensible for sequential deposition of several biomolecules on the same substrate in order to create intricate patterns of biomolecules relevant to neural development as well as biotechnology applications.
Any possible geometry is obtainable as it is specified by the mask.
We are using these stamps to define specific protein patterns for studying neuronal growth and development. The scanning electron micrographs below show a stamp having a grid pattern. We have successfully attached various types of proteins to glass surfaces. These and other protein patterns are used to control and guide the neuronal cells. We have used B104 neuroblastoma cells as an assay for the microstamping method.