The Transgenic Mouse Facility produces transgenic and gene-targeted mice for research laboratories of all three divisions at Fox Chase. Rederivation of existing mouse lines is also offered.
Assistance is available for experimental design, analysis, husbandry, and non-surgical techniques.
Transgenic mice are produced by microinjecting a gene construct, made by Center investigators, into the pronucleii of mouse zygotes, which subsequently develop to term after surgical transfer into a recipient female mouse. Mice in which the transgene has integrated into their genome are identified by the investigator's laboratory using Southern blot analysis. After surgical transfer and development to term, mice that carry the mutated ES cells are identified by coat color and transferred to the investigator for breeding. The facility occupies 235 square feet of laboratory space within the Laboratory Animal Facility. Ancillary equipment includes a tissue culture incubator, micropipette puller, microforge, microgrinder, and vibration isolation system.