Macromolecular Core Facility Protein sequencing Two Applied Biosystems 477A protein sequencers are available for sequence analysis. A Milligen 9500 Fmoc peptide synthesizer is available for multiple peptide synthesis. The cost is 75 per base, with no set-up charge.
Custom syntheses such as modified bases and phosphorothioate chemistries are also available.
The densitometric image obtained is then analyzed to locate individual bands, subtract background intensity, and calculate the integrated intensities of each band. A PDI workstation is capable of sophisticated analysis of both one and two-dimensional gel images, as well as slot and dot blot images. For direct quantitation of radioactive bands in dried gels or blots, a Molecular Dynamics PhosphorImager is available, with a linear range of 5 orders of magnitude, and analysis software similar to that of the PDI workstation. A wide range of faculty from the College of Medicine use this core lab, as do many outside contractors. The goal has been to offer fair market costs, with the charges for services used to offset chemical and maintenance costs.