The rough, circular Bacillus sp. Mix on a vortex mixer. Place 0.1 - 0.5 ml of soil slurry on a sterile nutrient agar plate. Spread the slurry with a spreading rod. Or look for actinomycete colonies.
Actinomycetes produce powdery colonies with hyphae embedded in the agar.
Isolates Photo CLCase Isolate one colony and inoculate it in a single line across a nutrient agar plate. Incubate the plate inverted under good growth appears. Inoculate different bacteria perpendicular to the line of growth. Be careful not to touch the existing bacterial growth. Look for zones of inhibition near the original growth.