This project sought to use a gene therapy approach to confer human T-cells with resistance to HIV infection. A t the point at which I left the project to come to UWEC, we had found that cultured cells, which had defective integrase genes introduced into their genomes, were resistant to HIV infection for up to three months. Better understanding of this enzyme could potentially lead to therapeutic interventions targeting integrase . These techniques will include cloning, expression of viral genes in bacterial systems, DNA sequencing, gel electrophoresis, PCR, and protein purification. Cancer cells have lost the normal controls that regulate cell division.
Control points exist immediately before and after the DNA replication steps of the cell cycle.
I am interested in the events that initiate, control, and terminate DNA replication.