The following is taken from a protocol utilizing sheep surgery. All surgical procedures are performed using halothane anesthetic and aseptic technique. All others entering the surgery room also wear hats, masks and shoe covers, in addition to non-sterile gowns or surgical scrubs.
All materials used in surgery are steam or gas sterilized.
Incisions are closed in layers. All external incision sites are sutured with non-wicking surgical material such as Braunamid or Vicryl, or with sterile wound clips. Silk is used for internal sutures, such as fetal skin sutures, and for ligatures. Muscular layers are closed with chromic gut. Sterile surgical clips are also used for ligatures where appropriate.