The knowledge gained from these studies will permit optimal r-HuEPO use. We are also pursuing early phases of collaboration with the Departments of Orthopedics and Geriatrics. In these studies, we hope to use the nonradioactive method for red cell volume in order to preoperatively identify individuals who are at a greater risk of needing blood transfusions associated with surgical procedures, such as arthroplasty, that can often lead to significant blood loss. If these individuals can be identified using our technique or with one of the modern composition techniques, such as photon absorptiometry and air displacement plethysmography, these individuals could donate their own blood for later post-operative use. Increased urinary excretion of 3-hydroxyisovaleric acid and decreased urinary excretion of biotin are sensitive early indicators of decreased biotin status in experimental biotin deficiency.
Biotin catabolism is accelerated in adults receiving long-term therapy with anticonvulsants.
Biotin physiology, dietary sources and requirements. Uptake and metabolism of biotin by human peripheral blood mononuclear cells. The efflux of biotin from human peripheral blood mononuclear cells. Bioavailability of biotin given orally to humans in pharmacologic doses. L.F. Burmeister, D. Kahn, and R.G. Strauss.