The facility provides DNA sequencing services using our ABI 310 single-capillary instrument. We normally use the BigDye terminator chemistry. The customer provides template and primer, we do the rest.
Date is provided on floppy disk or Zip disk.
We will also run samples for those users who prefer to do their own sequencing reactions. We need 3.2 pmol of primer for each reaction. Concentrations must be high enough that template and primer combined are less than 12 microliters. Free Software for viewing and working with DNA sequencing electropherograms is available.