Repeated freeze-thawing results in protein degradation. Before using these samples they should be warmed at 37 C for a few minutes to redissolve SDS which precipitates out of solution. A proper record should be kept for every gel and possibly a gel electrophoresis record made up similar to the one shown in the Appendix. Assign each gel a gel number. Wear gloves at all times when using acrylamide.
Acrylamide is a neurotoxin.
Even after polymerization some unpolymerized acrylamide may be present. If the skin should come into contact with acrylamide solution or powder, wash immediately with soap and copious water. The high electrical power used in gel electrophoresis is very dangerous as such one should never disconnect the electrodes before first turning off the power supply. Excess micelle formation. Minimize the time between sample preparation and electrophoresis.