DAG is an important metabolite during acrosomal exocytosis.
It has become clear from various studies that DAG has a central role as lipid second messenger. The presence of other types of PICs has also been inferred. However, recent studies have revealed that the acrosome may serve functions similar to those played by the endoplasmic reticulum in somatic cells. Inclusion of EGTA almost totally inhibited the enzyme s activity. Several studies have demonstrated that activation of PIC and hydrolysis of PPIs are essential for acrosomal exocytosis.