Different methods are in use for the quantitative determination of IgG subclasses.
This reliable assay is widely used and easy to perform. However, when large numbers of samples have to be processed the relatively long incubation times will be a disadvantage. Test samples, standard sera and control sera are prepared and added to the plates. After 48-64 hours incubation at room temperature the diameters of the immunoprecipitation rings are measured. It is not necessary to make a calibration curve.