Once you've got this relatively easy assay down you can plan an experiment. As we learned in class, flagella are organelles made of microtubules, which are composed of tubulin monomers. Flagella also have many other associated proteins that are important to their structure and movement. Mictrotubules are frequently polymerized and de-polymerized in the cell for different purposes and there is an internal pool of tubulin monomers that are used in this process. The internal pool of tubulin monomers not sufficient for the construction of two new flagella - the cell requires novo synthesis of monomers.
Calcium is important as a second messenger to the re-flagellation process.
Some studies have shown that de-flagellation can protect the cell from heat death. You can choose any one of these questions and build a hypothesis on it. Alternatively, come up with your own question and make a hypothesis. Either way, design an experiment to test your hypothesis. Click here for some other information that will help in your experimental design.