It causes weakness and dysfunction of the back quarters and leads to painful arthritis. Outbreak of the disease is influenced by environmental factors. Today hip dysplasia is diagnosed by radiography. The resulting species specific banding pattern is used to identify and characterize individual chromosomes. In our studies a higher number of red blood cells was observed to compensate the decrease of oxygen uptake that is caused by the narrowed upper respiratory tract.
Detection is carried out by complementary probes conjugated to a fluorescent compound.
The method of real-time PCR has been developed to measure input target templates quantitatively. PCR quantitation has been used in a wide variety of applications, including the evaluation of gene copy number and mRNA expression, the diagnosis and management of infectious diseases, to investigate the efficiency of gene delivery systems, to verify biological product purity and to qualitatively detect genes and alleles in high-throughput assays. Therefore microsatellites are ideal identity markers. After carrying out fluorescence labeled multiplex PCR, the amplificates are electrophoretically separated and automatically detected. The gel image shows the results for 11 different bovine microsatellite loci applied per lane.