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The objective of this study was to determine whether medium supplementation by antioxidants and fatty acids would improve the viability of cultured rat hepatocytes and protect them against doxorubicin toxicity. The mixtures were added 18 hr prior to, simultaneously with or following treatment with doxorubicin and just simultaneously with CCl4. Neither vitamin E, sodium pyruvate nor egg yolk alone improved viability. 24 hr later. The mixtures did not protect against toxicity caused by CCl4, perhaps due to the overwhelming level of damage at its EC50 concentration.