After processing, the specimen is embedded in paraffin and blocked. Accelerated Processing Block the wax and specimen out in a mold. Cut sections on a clean microtome with a clean blade. Use disposable knive blades if possible. Mount on clean non-coated or coated glass slides.
Air dry the paraffinized sections overnight.
In some cases, baking for up to 8 h at 37 or 42 degrees C can be recomended. Some histopathology labs use an adhesive in the water bath to better adhere the tissue section to the slide, and this may result in reduced LCM transfer of tissue. For a successful LCM transfer, the polymer film must be bonded to the targeted tissue so it forms a stronger bond than that between the tissue and the underlying glass slide. Also, make sure to mount the tissue as close to the center of the slide as possible. If the tissue is not mounted in the center of the slide, it may be difficult to lock the slide down to the stage and microdissect the area of interest.