It is preferable that such tubs can be easily sterilized, such as by autoclaving, to prevent cross-contamination of genotypes. Dry material for several days. We dry material in an unlit growth chamber at ca. 30 C for about 48 hours. Decant released spores from glassine envelopes while retaining sporophytic material.
Agitation can help to release additional spores.
Small batches of dried fertile fronds are placed onto a No. 40 sieve and lightly ground with the base of a 25 mL graduated cylinder. This grinding action should break up the fertile frond material, but not compltetly pulverize it and release nearly all of the spores. 60 sieves to the collection pan while the fertile frond material is retained. Stores spores in scew cap containers. All rights reserved.