All membranes seem to be absent.
There are halos where membranes should be but no membranes. I am going to try making up the fix in the culture medium next. Has anyone any thoughts of anything else to try. What are your fixation conditions - monolayer, pellet of cells. I wouldn't add fixatives to the culture medium, depending on its constituents you might have a Canazarro type reaction and fix the medium as well as the cells.