Clostripain Clostripain Clostripain is a sulfhydryl proteinase associated with collagenase and isolated from Clostridium histolyticum . It is highly specific for the carboxyl peptide bond of arginine. Studies on the active site are reported by Porter et al . Citrate, borate, and Tris anions partially inhibit. TLCK reacts with active site.
Calcium ion is essential.
One unit hydrolyzes one micromole of BAEE per minute at 25deg. C and pH 7.6 under the conditions specified. Enzyme Prior to assay, the enzyme must be activated. Activation appears to be complete within 2-3 hours at room temperature or overnight at 5deg. Procedure Adjust spectrophotometer to 253 nm and 25deg.