Administration of sequences and annotation of fully sequenced cDNAs is done at MIPS . All data is stored in the object oriented database system ObjectStore . Hereby data are stored into a set of different database components. An economic and efficient way, based on a three step decision procedure has been chosen to pick novel full length cDNAs of interest. 2 shows the principle of the decision process in more detail.
Automated actions are in yellow, human interactions are in blue coloured boxes.
Step 1 is the systematic sequencing of 5'-ends of all clones from different chromosome and tissue specific libraries, performed by the eight laboratories of the cDNA consortium followed by an extensive automated bioinformatical analysis. Additionally, ORF visualisation and analysis of the derived aminoacid-sequences are performed. Sequences which consist of pure vector, transposable elements or a high number of N's are automatically discarded, thus the biological reviewer is not occupied processing non-informative sequences. After that procedure, the sequences pass several client specific steps of publication until they are released to the public databases . Our public cDNA database contains additional information to the individual cDNA sequences.