Drug application at multiple holding potentials . Drugs are applied using hardware controlled through digital lines from the ITC-16. The decay constants of multiple exponential functions are extracted from the curve during drug application or wash-out. The resulting data are saved in the form of a user defined table. Standard deviation and variance curves can be computed interactively.
Conditioning pulses with variable delay to a test pulse .
A conditioning pulse of fixed or variable width is presented followed by a variable delay. Then a test pulse is presented yielding a family of curves having a decay component that is sensitive to the delay or variable pulse width. The curves are displayed, printed and selected for analysis. Time constants of multiple exponentials are saved in a table that are further analyzed by a statistical analysis program. Here is shown a diagram of the analog and digital outputs in a protocol having incrementing time.