These studies utilize biochemical enzyme assays to assess the metabolic activity of the mice for specific enzymes. Mutations at critical regulatory genes that control cell growth are assessed by analysis of gene structure for mutations that alter gene function. Immunohistochemistry is used to assess the relative levels of protein expression in tumors. Figure Legend Single strand conformation polymorphism analysis of exon 2 of the INK4a gene. The gel was vacuum dried and autoradiographed overnight.
Arrows indicate band shifts indicative of genes containing point mutations.
A second area of interest that is being pursued in my laboratory has been to extend our rodent work into human tumors. We currently have a pilot project ongoing to assess the effect of drug metabolic enzymes on the relative levels of gene mutations in human breast cancer patients. These studies may aid in the development of effective prevention strategies for individuals based on genetic risk factors. Students rotating through my laboratory will be taught the basic techniques of molecular biology and, depending on the length of the rotation and the student's interest, some of the more advanced methodologies for gene mutation analysis. Role of tumor suppressor genes in transplacental lung carcinogenesis.