To use the primer walking service, the client provides the Facility with a sufficient amount of clean DNA to complete the project. For subsequent sequencing and synthesis orders, the facility will log-in the work through your OnCore account so that you will be notified of the progress and can download your results as they are generated. Facility personnel begin by using universal primers or provided custom primers to sequence the DNA from each end. The next two primers are designed at 450-500 bases from the sequence data, depending on the data quality. The process is repeated until the sequences overlap in the middle of the DNA fragment.
Customers also can request that both strands of the DNA be sequenced.