This property is also kept by the hybridoma cells. But they have the additional property of antibody production and secretion. In an intermediate step is a wanted cell line isolated and cultured. Consequently are only very few antibody molecules bound, often is the antigen-antibody reaction at the limit of provability. Detection of Enzyme Activities.
Different methods exist.
Most common is autoradiography. For this, a radioactively tagged substrate or, even better, a substrate analogue that is bound by the enzyme but not converted is needed. After washing off the material that has not been bound can an autoradiogram be produced with the help of a special film that shows the position of the enzyme. The method is especially well established with membrane bound enzymes. Another possibility is the use of a substrate that is processed and renders an insoluble, stained product.