Similar results were achieved with Borrelia burgdorferi - stimulated splenocytes. This immunosuppression facilitates the transmission of pathogens by ticks. These results support the hypothesis on the immunomodulatory effect of SGE based on the stimulation of the Th2 cytokines, possessing the definitive anti-inflammatory effect. This also applies to cytokines induced by borreliae. Control means untreated splenocytes.
Control means untreated splenocytes.
In case of PS cells TBE virions are located inside RER cisternae while in tick cells the virus particles are located exclusively inside vacuoles. The tick cells were significantly more vacuolated in comparison with the mammalian PS line cells. The comparison of the basic pathogenetic parameters of the infected cells at the ultrastructural level resulted in the conclusion that in the mammalian PS cells the virion maturation is of the trans type while in the tick cells it is of the cis type. A total of 163 nymphal and adult Ixodes ricinus ticks were collected in South Bohemia and examined for borreliae by dark-field microscopy. A monoclonal antibody specific for B. afzelii was prepared and deposited in our collection of species-specific antibodies against B. burgdorferi sensu lato.