The Facility's synthesis specialist, Gary Polking, can design primers quickly and at a reasonable cost. To use the primer walking service, a customer provides the Facility with a sufficient amount of clean DNA to complete the project and information about the DNA, such as the size of the DNA insert and the vector used. Facility personnel begin by using universal primers to sequence the DNA from each end. The next two primers are designed at 450 to 500 bases from the sequence data, depending on the data quality. The process is repeated until the sequences overlap in the middle of the DNA fragment.
Customers also can request that both strands of the DNA be sequenced.
The new microscope can image stable or delicate biological specimens composed of low atomic number elements. It can reduce or eliminate charging and will enhance surface detail. The JEOL 5800LV was purchased through a grant from the National Science Foundation with matching funds from Iowa State University. Biotechnology Adds New Dimensions to Botany David Oliver , new Botany Department Chair and biotechnology researcher says that botany is a exciting field in which to apply the molecular tools of biotechnology. One of his major projects is a study to determine how mitochondria, the energy storehouses of cells, change as plants develop.