The plant biologist has expertise in plant immunocytochemistry and biochemistry. The T7 RNA Polymerase is a viral enzyme that only recognizes its own promoter sequence. First, it is a very high activity enzyme and is efficient at producing large amounts of RNA that is then translated into protein. Briefly describe the primary research objective of each of the 4 steps in the diagram. Immune reaction in the rabbit to produce anti-calreticulin antibodies.
Antibodies are specific reagents to bind to plant calreticulin proteins.
List the probable protein sources for the following lanes in the protein gel. The eluted antibody is highly specific for calreticulin and enriched compared to the unpurified serum. All rights reserved.