Easy to use Ultraffinity-EP is based on a macroporous silica to which is bonded a hydrophilic spacer terminating in an active epoxide function. This result in a combination of superior physical characteristics and chemical properties well suited to affinity chromatography. The column is suitable for strong and weak ligand macromolecule interactions, yet non-specific adsorption is reduced. In addition to primary amines, the epoxide function will also react with sulfhydryl and hydroxyl groups. Leaching of ligands is minimized.
Biological activity is preserved.
Some separations require specialized Altex columns and packing materials. These special applications include carbohydrates, amino acids, and organic polymers. The basic HPLC separation mode for large molecular weight compounds is size exclusion. Specific peaks of interest can be collected for further analysis by ion exchange or hydrophobic interaction chromatography. The mobile phase pH can be as high as 12.