MPIZ has started the use of fluorescence-labelled ISTR primers for automatic DNA marker analysis on sequencing machines. A first population of 52 F1 plants from one of the MYD X LAG crosses was available in January 1997 and served as material for the construction of a first ISTR coconut map. An initial analysis of this mapping population identified 51 polymorphic ISTR markers 43 of which could be arranged into 12 linkage groups comprising a total of 542 recombination units. The possible identification of molecular markers cosegregating with desirable traits Unselected F1 populations of controlled crosses were not available to the project. It is foreseen that ISTR will be useful for breeders in EC countries as well as for DC partners in their efforts to analyze national plant genetic resources for their conservation and exploitation.
Coconut germplasm characterization using DNA marker technology.
Randles et al. Rohde et al. Rohde et al. Rohde et al. The calculation of recombination frequencies in crosses of allogamous plant species with application to linkage mapping.