After 1-5 days, use dishes with confluent monolayer in flow assay. Feed cells every 2-3 days, but usually not with 48 hrs. Coated Substrate Preparation 1. Incubate 1 hr. Aspirate off BSA, add 20 l of inhibitor for 1 hr. 5.
Dishes are ready for use in flow assay.
Flow Assay Using Parallel Plate Flow Chamber 1. Assemble flow system apparatus connecting inlet, outlet, and vacuum lines to the flow chamber deck. Fill system with media and remove all air from system. Fill inlet reservoir with cell suspension. Attach dish to flow chamber deck by holding the deck inverted, place a small bubble of media on flow path area, then place 35 mm dish on the deck.