Nature Biotechnology 15, 803. 2. Hawkins, G.A. et al. The amount of PCR product will be about the same as with a traditional nucleotide mix. If necessary, the PCR should be optimized using the BESS PCR Optimization Kit until each primer pair yields only one PCR product for BESS analysis. Separate and visualize the BESS reaction products just as you would for a dideoxy sequencing reaction.
Asterisks indicate sites of mutational change.
Inc. Figure 2. G to T point mutation in codon 1250 on an ABI 310 DNA sequencer. The mutation in codon 1250 results in a Glu to ter change. Contact your local Epicentre Distributor .