Linear correlation analysis was used to compare signal intensities of hybridization to each element by the same target on two different slides. Log10 transformed ratios of hybridization signals for both targets on two different slides were also compared. Pearson correlation coefficients ranging from 0.77 to 0.87 indicate the reproducibility in hybridization between replicate Microarrays. As expected, correlation coefficients demonstrated that hybridization was more variable between slides than between replicates. Signal intensity values ranging from low to high are represented by color changes from blue to green, yellow, red and white, respectively.
Black areas have no detectable hybridization signal.
Signal intensity data for this image can be downloaded in text format. The 605 Endosperm Microarrays are composed of eight subarrays arranged in 4 rows and 2 columns. Each of the 8 subarrays is composed of a 46 x 46 matrix of spots printed at a spacing of 190 um with a 380 um gap between subarrays. Each DNA element on the slide can be located by knowing the Subarray Row and Subarray Column and the Row and Column within the subarray where the element is printed. For more information about the format of the 605 Microarrays visit the information button on the Microarray Elements Search page.