In lieu of individual abstracts, research projects and investigators at the LANL Center for Human Genome Studies are represented in this narrative. More information can be found on the center's Web site . Eventual establishment of the national genetic sequence databank called GenBank, the National Flow Cytometry Resource, numerous related individual research projects, and fulfillment of a key role in the National Laboratory Gene Library Project all contributed to LANL's selection as the site for the Center for Human Genome Studies in 1988. Extensive interdisciplinary interactions are encouraged. Specific work includes the construction of high-resolution maps of human chromosomes 5 and 16 and associated informatics and gene discovery tasks.
All available loci continue to be incorporated into the map.
Refinement of BAC cloning procedures for future production of chromosome-specific libraries. Successful partial digestion and cloning of microgram quantities of chromosomal DNA embedded in agarose plugs. Efforts continue to increase the average insert size to about 100,000 bases. SASE data is deposited in publicly available databases to allow for wide distribution. Finished sequencing is prioritized from initial SASE analysis and pursued by parallel primer walking.