The enhancer first condenses the plasmid DNA and the effectene reagent, non- liposomal lipid, subsequently coats the condensed DNA for efficient uptake into cells.
Cell transformation is an essential tool for molecular, cellular, and genetic research.
By examining the effects of the exogenous molecule on the cell, scientists can trace the pathways of a wide variety of protein synthesis, enzymatic, and immune system processes. Transformation of human cell lines in vitro has shed light on the causes and progression of autoimmune diseases, aging, cancer, and other conditions prior to in vivo testing of candidate drugs. Although the considerations below apply in general to transformation experiments involving any type of molecule for uptake into any suitable target cells, this article focuses on transfection into human cell lines. The measure of a successful cell transformation is high efficiency. The challenge is to maximize the number of cells that are viable after transformation and incorporate the exogenous molecule of interest in the cytoplasm or nucleus.