Thus, Prp40 appears to play a central role in early splicing events. Indeed, we have recently shown that Prp40 interacts directly with other splicing factors early during the splicing pathway. We are now using genetic and biochemical techniques to define these interactions, as this information will shed important new light on how the splicing reaction achieves its high specificity. RNA sequences upstream of the 3' splice site repress splicing of mutant pre-mRNA introns in yeast. The interaction between the first and last nucleotides in the second step of pre-mRNA splicing is independent of other conserved intron nucleotides.
Acids Res .
The amino terminus of the U1 snRNP protein 70K is necessary and sufficient for function in yeast. Copyright 1999 by the Regents of the University of Minnesota.