Reported population densities are 0.5 per ha. Home ranges are relatively small, recorded averages varying from about 0.053 ha. Home ranges apparently overlap extensively, and territorial behavior is not evident. Ochrotomys is a fairly sociable rodent, and up to 8 individuals have been found in the same nest. The reproductive period seems to vary considerably, having been reported as throughout the year in Louisiana, mainly from September to spring in Texas, and from March to October in Kentucky and eastern Tennessee.
Captives breed throughout the year, though with marked peaks in early spring and late summer.
Several litters may be produced annually in the wild, and some captive females have borne as many as 17 litters within 18 months. The gestation period is about 25-30 days. Litter size ranges from 1 to 4 young and averages about 2.7. Captive females have produced young when as old as 6.5 years and have lived as long as 8 years and 5 months.