Introduction to modern techniques used for protein purification and characterization. Emphasis on methods for purification of fusion-proteins. Techniques include gel electrophoresis, immunoblotting, determination of protein concentration, cell fractionation, principles of centrifugation, and column chromatography. Prepares for MCDB 475 or 495. Physics majors are welcome.
Class size is limited.
Students must consult the instructor prior to registration. Advanced laboratory techniques and research strategies common to several disciplines, including biochemistry, physiology, cell biology, video microscopy, and digital imaging. Past modules have included the purification and characterization of microtubules, calmodulin, and myosins. Participation in one or more mini-modules focusing on current research from the laboratory of a senior faculty member. Toward the end of the term each student pursues an independent mini-project involving the purification of a protein of interest.