Ripe for bacterial infections, used to result in death by 20's. As described previously, use RFLP analysis to get close to gene. Protein normally functions to regulate Cl and Na transport. Examine Fig 26-14 3bp deletion cause majority of defects.
Answer is yes.
These are real life difficult questions, especially with regard to epithelial cells in airway - top cells will continuously slough off, so that treatment will not be permenant cure. Use of retrovirus to stably integrate normal gene into target cell Fig 12-13. Very little chane of recombination to allow for production of viral progeny. Fig 28-2 use targeting vector to specifically recombine defective gene with region it lacks. Note different Southern blot patterns.