Buds should be transferred to fresh nutrient solution if there is algal contamination.
BUDS Collect smaller marginal frond buds from greenhouse plants.
Buds should be no larger than about 1 cm and most of the leaf tissue from the mother plant should trimmed into the shape of a small triangle. In addition, leaf tissue from the bud should removed. As buds are collected, float them in water in a styrofoam cup covered with a plastic sandwich bag. Carefully transfer buds to the bottom of a sterile 50 mL centrifuge tube, i.e. do not touch sides of tubes. Do not sterilize more than about 50 buds at one time.