Different sources say different things. It is getting unclear when I am supposed to think bacteria and when I am supposed to think eukaryote. Its action is so critical that inhibition by ciprofloxacin is lethal. The combined action of topoisomerase I acting just ahead of the replication fork and the gyrase underwinding action further down the helix are needed to remove the positive supercoiling strain. Primase does not have the 3' to 5' exonuclease activity but the DNA polymerases do.
Different sources say different things.
It is getting unclear when I am supposed to think bacteria and when I am supposed to think eukaryote. Its action is so critical that inhibition by ciprofloxacin is lethal. The combined action of topoisomerase I acting just ahead of the replication fork and the gyrase underwinding action further down the helix are needed to remove the positive supercoiling strain. Primase does not have the 3' to 5' exonuclease activity but the DNA polymerases do.