You separate the pellet and supernatant in the same way as before. This time you find that if you add the appropriate translational factors to the pellet, you can successfully detect protein synthesis. In your laboratory you are able to follow the path of acetylcholine within the cell, and see that it is first synthesized, then chemically modified, stored for a while, and eventually transported to the plasma membrane. Acetycholine Receptor Protein takes in neurons, showing which organelles are involved in each step. Draw a colored diagram.
Cilia are important cellular structures exclusively possessed by eukaryotic cells.
Some eukaryotes like Paramecium are fully dependent on cilia for motility and food particle retrieval. When examined in ultrastructural detail, cilia appear to have bundles of microtubules arranged in an orderly manner. To test if this really is the case, you decide to carry out an experiment. Then you label your protein with a red fluorescent dye. In the next step, you incubate your microtubules and fluorescent labelled protein under conditions that favor microtubule assembly.