The facility uses ABI 377 and 373A Stretch sequencers, with BigDye Taq FS Terminator or Dye Primer chemistry. Please consult our recommendations for template preparation to avoid failures. Custom primers should be provided by the user, or can be synthesized at the facility. Users can choose between long or short runs. Short runs give a total read length of 600 nucl.
Charges vary according to length of run and extent of service.
Turn around time is generally 2 working days for sequencing requests. Color printouts of analyzed chromatograms are provided for all successful reactions. Sequencing results can also be obtained on disc or over PennNet for further examination and manipulation by the user. Facility staff will consult with users whenever there are problems, and will make suggestions to improve sequencing results. This allows us to process samples using a robot for pipetting reagents.