ACL specialises in the development and manufacture of adsorbents tailored to the current needs of the biotechnology and medical device industries. In addition to an exclusive range of products, a high quality yet inexpensive custom development and synthesis service is offered for the manufacture of adsorbents which are ideally suited to your own requirements. Affinity Chromatography Affinity chromatography, or bioselective adsorption chromatography, has become an indispensable tool for the isolation of proteins. As demands placed on protein purity continue to increase, traditional purification techniques such as ion-exchange or gel permeation chromatography can no longer meet purification requirements of industry and academia alike. There is now a real need for selective adsorbents which offer high degrees of purification and high protein binding capacities, whilst at the same time are highly stable and available at an affordable cost.
Affinity chromatography offers several advantages compared to conventional purification techniques.
An affinity adsorbent allows selective adsorption and desorption of proteins, so providing a considerable degree of purification. Increases in purity of over 1000-fold are not uncommon. In addition, the protein is concentrated and frequently stabilised upon binding to the immobilised ligand which often leads to high recoveries of activity. Consequently, affinity chromatography is ideally suited to processing large volumes of protein containing solution. The use of affinity chromatography in a purification procedure can dramatically reduce the number of steps required for purification.