Plastic is nonporous, which greatly decreases nonspecific binding, so you get the benefit of a clean background with minimal washing. The plastic microarrays are also rigid and do not warp when exposed to high washing temperatures, so they can be thoroughly stripped and reprobed several times and still retain their original configuration. Since the film does not distort, template alignment and image analysis are much easier than analysis of data of nylon membrane-based arrays. Additionally, the spots on this surface yield a uniform signal intensity distribution, which is critical for accurate automated data processing. A closer look at the expression profiling experiment shown in Figure 1 clearly shows differential expression.
Arrows indicate a small subset of differentially expressed genes.
As with our other Atlas Arrays, the plastic microarray contains housekeeping genes and negative controls. Genes per array Cat. Inc. All rights reserved. For International orders, click here . Privacy statement and Disclaimer .