Add 1.2 M sorbitol. The medium is adjusted to pH 5.5. Supplements added as for YES except lysine.
All media is prepared in bulk.
It is sterilized by autoclaving at 10 psi for 20 min. At this pressure very little caramelization of glucose takes place. The media is stored in 500ml bottles and agar is remelted in a microwave oven before using. Return to the index of Section 1 . All rights reserved.