Centricon-100 concentrator spin columns from Amicon are recommended. C. Avoid primers that can have secondary structure or can hybridize to form dimers. Custom purified primers are recommended. Highly recommended are E.coli DH5a and HB101 strains. Jm109, XL1-Blue and MV1190 strains are also recommended.
Customers are responsible for the quality of DNA to be sequenced and custom sequencing primers.
Tubes should be labeled with template name or primer name. The contracting Lab will supply M13F, M13R, T3, T7, or SP6 primers for a sequencing order at no additional cost.