At least four working standards should be prepared, so that a curve of concentration versus response can be plotted. Samples should be bracketed with working standards. Liquid chromatograph conditions. Peak areas are measured by an integrator or other suitable means. A calibration curve is constructed by plotting detector response of standard injections versus ug of diethanolamine.
Any compound having the general retention time of the analyte is an interference.
Possible interferences should be listed on the sample data sheet. LC parameters should be adjusted if necessary so these interferences will pose no problems. Retention time data on a single column is not considered proof of chemical identity. All units must cancel. The above formulas can be consolidated to make the following formula.