Amino Acid Analysis Sample Delivery Requirements Please fill out and submit the Amino acid Analysis Request Form along with your samples. The absolute minimum sample needed is 0.1 ug.
Accuracy increases with quantity.
If you want to submit a sample in a non-volatile or amine salt buffer because you think the amount of salt will not affect the analysis, we strongly suggest that you also submit a separate aliquot of buffer and ask us to amino acid analyze a control peptide or protein in that buffer to ascertain its effect on the analysis. The alternative is post-column derivatization chemistry in which amino acids react with a reagent such as ninhydrin after they elute from an ion exchange column. Pre-column derivatization chemistry, although more sensitive, is subject to interference from the sample matrix. This chemistry is most suitable for samples that do not contain large amounts of interfering substances, making it very important to consider your final purification methodology. Samples should be free of detergent, amine salts and lipids.