A majority of trainees produce transgenic mice during training. Blastocyst Microinjection Participants will learn all techniques necessary to produce embryonic stem cell-mouse chimeras. A minority of trainees produce chimeric mice during training. Mouse Embryo Transfer Participants will learn all techniques necessary to transfer mouse embryos to the reproductive tract of recipients. This includes superovulation, isolation of pathogen free fertilized eggs from infected stocks, transfer pipette fabrication, identification of pseudopregnant females, surgical transfer of eggs to pseudopregnant recipients.
All trainees produce offsrpoing from transferred eggs during training.
We guarantee the production of transgenic mice, but can not guarantee that any given transgene will be expressed in transgenic mice. We guarantee a minimum number of blastocysts will be injected with ES cells, but can not guarantee that any given ES cell clone will form germline mouse chimeras. The Genetically engineered animals provided by this facility can only be used for research purposes. Access to cell lines and plasmids is only provided to investigators who have complete, approved material transfer agreements .