The number of mutations that have accumulated in the sequence indicates that it must have been in cells for millions of years. You have a restriction map of both plasmids, as illustrated in Figure 1, so you take a sample from tube 1 and digest it with the restriction enzymes EcoR1 and BamH1. When you separate the DNA fragments using agarose gel electrophoresis, the fragments show the bending patterns illustrated in Figure 2. S Tube 1 Tube 2 5.0 kb 1.2 kb 1.0 kb Figure 2. DNA Agarose gel.
Boxes indicate DNA bands.