Registration deadline is May 8, 1998. We have successfully tested all of these scales. Salt or pH gradients are software generated and monitored, and multiple-component buffer systems are automatically assembled. Absorbance is monitored simultaneously at three wavelengths, user selectable from 180 to 700 nm, as are conductivity, temperature, and pH. Fraction collection is also flexible, from sub-milliliter to liter volumes, or collection only of peaks can be selected. Software for peak integration and quantification is installed.
All data collected during a run are available for inclusion in customized graphs and reports.
These new dyes contain a unique structure consisting of a fluorescein donor dye linked to a dRhodamine acceptor dye Figure 1. The donor dye is optimized to absorb excitation energy of the argon ion laser in the automated DNA sequencer. The emission spectra of the dRhodamine acceptors are also narrower than standard dye terminators giving less spectral overlap and therefore less noise. The combination of brighter signal and decreased noise provide an overall 4 to 5-fold improvement in sensitivity. Also, the peak height pattern will be more even than with traditional dye technology.