The purpose of our work is to understand how biological systems that contain metal centers work. Detailed schematics available in Rev. Sci. B. Nitrite Reductase . Activated bleomycin is the oxygenated form of bleomycin necessary to induce the free radical by which DNA is broken. D. Paramagnetic Centers of Cytochrome Oxidase .
Cytochrome oxidase is the essential terminal enzyme of aerobic respiration from yeast to man.
This work, directed at finding electronic structural details of partially reduced intermediates, is beyond the power of protein x-ray crystallography to resolve. We collaborate in this work with Dr. R. Gennis, Dept. of Chemistry, U. of Illinois. Integrating the DR to an adjacent rapid-mixer provides for the kinetic detection of paramagnetic species on the millisecond time scale. A submillisecond micro mixer is in the testing stages. Details described in Rev. Sci.