Centrosomes move apart. Nuclear envelope fragments, which allows microtubules to interact with the highly condensed chromosomes. Each chromatid now has a specialized structure, the kinetochore, located at the centromere region. Kinetochore microtubules become attached to the kinetochores and put the chromosomes into agitated motion . Centrosomes are positioned at opposite poles of the cell.
Chromosomes move to the metaphase plate, the plane equidistant between the spindle .
Centromeres of all chromosomes are aligned on the metaphase plate. Kinetochores of sister chromatids face opposite poles, so identical chromatids are attached to kinetochore fibers radiating from opposite ends of the parent cell. Entire structure formed by nonkinetochore microtubules plus kinetochore microtubules is called the spindle. Anaphase Anaphase is characterized by movement. It begins when paired centromeres of each chromosome move apart.