Method for Deriving . . . A method for the identification by modeling of synthetic catalytic RNAs and ribozymes thereof has been derived including a hairpin portion and binding sites for cleaving an RNA substrate at a site which has a GUA sequence following the site of cleavage. Both RNA catalytic centers are novel. These two centers have a preference for target sequences containing GUA following the site of cleavage. The sArMV ribozyme also cleaves target sequences with GUG ollowing the site of cleavage.
Catalytic activity is quite high.
Both ribozymes can be extended in helix 1 to cleave new target sequences.