Cell culture inserts in 10 mm and 25 mm with nonautofluorescent, highly porous, 0.02 m Anopore membranes are also available, as are 8-well strip inserts. Rollin', Rollin', Rollin' Roller bottles are used for large-scale cell growth and virus production. Bottles are made of polystyrene or PETG in a one-piece seamless design. Bottles are made with a smooth or pleated surface, and most are available with or without vented caps. Advantages of roller bottles over flasks are the enlarged growth surface, the slow and steady mixing of the medium over the cells, and the increased medium surface contact versus medium volume.
Bottles are typically tissue culture treated for adherent cells.
BD Biosciences' TufRol roller bottles are manufactured using a special process that yields a stronger polystyrene. Bottles are available with an 850 or 1,450 cm 2 growth surface. Easy grip caps are available with and without venting. Greiner's CellMaster roller bottles are currently made of PETG and available in an 850 cm 2 size. Polystyrene bottles and larger sized bottles will be available soon.