The number of taps is limited to 3. The third tap should be performed under anesthesia or after euthanasia. An increase in the number of taps permitted may be obtained after demonstration to the ACUC that the specific tumor line does not cause significant pain or distress to the animal in the proposed time frame. Consideration should be given to fluid volume replacement to avoid hypovolemic shock if large volumes of ascitic fluid are removed. Mice should be monitored for 20-30 minutes following a tap and again later on the same day.
Animal is lethargic, dehydrated, inappetent, maintains a hunched posture and has a ruffled haircoat.
Pilot studies are recommended for new cell lines to determine the growth characteristics of the cell line and optimum time frame prior to use in a larger number of animals. B. All tumor cells should be screened prior to injection to prevent transmission of common rodent viruses. Amyx, H.L. 1987 Control of animal pain and distress in antibody production and infectious disease studies. A comparison of commercially available adjuvants for use in research. Parameters affecting ascites tumour formation in mice and monoclonal antibody production.