Abstract A discussion of microcalorimetric methods for the detection and analysis of biomolecules.
Detection is based upon ultrasensitive and precise measurement of heat absorbed or released.
MicroCal's VP-ITC can determine binding constants, enthalpy, entropy, heat capacity, and the number of binding sites of unmodified reactants in homogenous solution, and has found increasing application in the functional analysis of protein, lipid, and nucleic acid interaction and modeling of binding to cell membranes. ITC is also used in antibody quality control and characterization, and modeling of ligand interaction with cell surface receptors.