Two weeks later they were euthanized by CO2 inhalation. Twenty euthymic female guinea pigs were inoculated intravaginally with the Herpes simplex virus. An appropriate volume of an isotonic solution was instilled into the upper tracheas of three dogs and promptly removed. The dogs exhibited no signs of pain and only minimal distress from the procedure. Five hamsters are inoculated intraperitoneally with a hybridoma cell line and the ascitic fluid is removed from the peritoneal cavity three times.
All hamsters are alert, active, and eating and drinking normally.
The hamsters are euthanized and the remaining ascitic fluid removed postmortem. Periorbital collection of blood in species without a true orbital sinus, such as rats and guinea pigs. Intracardiac blood collection. Euthanasia performed in accordance with the recommendations of the most recent Report of the AVMA Panel on Euthanasia utilizing procedures that would involve pain or distress if anesthetics, analgesics or tranquilizer drugs were not used, such as pithing in certain species and exsanguination. Twenty dogs were assigned to an ASP involving a major survival surgical procedure, but two were actually used as nonsurgical controls and experienced no pain or distress.