Primer Extension sequencing covers any project using known primer sequences in which sequencing reactions are being performed only from the ends of the plasmid or PCR product. SeqWright will perform individual sequencing reactions using a primer provided by the customer or a universal primer. The customer can provide their own primer or SeqWright can design and synthesize a primer for them. The turn around time for this service is between 24 - 48 hours.
Discounts are available for large scale projects.
The DNA of interest may be either a plasmid insert or a PCR product. The initial sequencing will be performed from each end using either universal primers or primers designated by the customer. In order to completely sequence the region of interest SeqWright will design and synthesize primers as necessary to obtain contiguous sequence information. Single Strand Primer Walks Single strand primer walks provide contiguous sequence information for one strand of the region of interest. Due to the fact that only one strand is sequenced we can only guarantee an accuracy level of between 95 and 98 percent.