It takes us 3-5 minute to finally process the blood to become plasma. We are looking some esterase inhibitor to stop the reaction once the blood is withdrawal. But so far no luck. I'm very interested if someone could give me any suggestion about how to overcome this problem or some experts' names so that I can contact to get help. I would greatly appreciate any help I could get.
Company is also willing to compensate the cost if it is required.
Many esters are not stable under physiological condition so the first thing that needs to be considered is whether conversion is truly enzymatic. Try collecting blood into cooled tubes. Alternatively, hydrolysis may be base catalysed and a low pH buffer may be suitable. If hydrolysis is indeed esterase mediated, chances are that it is a serine hydrolase. Rapid mixing will be required during collection.