TSO is not as simple to setup as the standard Vantage in terms of drop stability, etc., but my guess is most operators would probably pick it up quickly. What we think - so far We really like it . So far, we have been able to at least double the flow rate for most sorting experiments. Many sorts that would usually need 8 to 10 hours of Vantage time are being done in 3 to 4 hours without any loss in sorting efficiency. To illustrate, compare sort recovery values for standard and TSO Vantage parameters.
Care is taken when adjusting the dead time and timing the deflection packets.
We hope to have results soon from some proliferation assays being done on cells sorted at high velocity. The improvement is significant, no huge differences in sorting efficiency when in multi-laser mode. The new board allows for up to 3 laser beams in 3 different spatial positions, although we are still using two spatial positions.