We are also attempting to undertake experiments to directly test the capability of aminoacylated RNAs to conduct protein synthesis in the absence of ribosomes. Several more practical projects that draw from this basic research are also being pursued. One of these is the use of stable RNAs derived from 5S rRNA as monitoring tools for tracking genetically engineered microorganisms in the environment. We have developed an expression system that allows rapid construction of such RNAs. We are also studying the use of rRNA targeted probes for microbial detection in space environments.
Acids Res .