Therefore, CnA and CnB interact only in the presence of 1 mM calcium but not in low calcium conditions. CnB has 4 calcium binding domains and these allow CnB to bind to CnA when calcium is in high enough concentration. This question has some figures that are not necessary to answer the question, but help you understand the importance of this work. Go to the same URL to see the four additional figures. Read the abstract.
Angiogenesis is the biological process of forming new blood vessels.
Remember that blood vessels are lined with endothelial cells. I will tell you that this group started with 20 liters of mouse urine, so you know it is not found in high concentrations. First, I know it is probably a protein since boiling it destroys its activity. So I am looking for a protein. I would continue to purify the active protein away from the non-active proteins.