There is a total of 24 in this Gospel. Until a sinner is regenerated or born again he is spiritually blind, dead and an enemy of God. He cannot help himself. The Law cannot cause rebirth. Only the Gospel does that.
Baptism causes rebirth.
That's why Jesus commanded that all nations be taught and baptized. 6 tells us that physical birth only produces sinful flesh but birth caused by the Holy Spirit causes a person to be spiritual. The Spirit's gift of faith in Christ causes rebirth, a new creation. Nicodemus became a Christian. Jesus is neither flattered by Nicodemus nor does He talk down to him.