Monoclonal Antibodies Unique proteins that recognize and attach to specific foreign substances in the body . Spleen cells are mortal but produce antibodies while myeloma cells are immortal but have no ability to produce asntibodies. The resulting hybrid cell will have both of the above properties. Screen the cell population for hybrid cells. B-Lymphocytes produce immunoglobulins known widely as antibodies.
Antibodies neutralize specific pathogens and prevent spread of infection and reinfection.
Set up conditions so that only your hybrid cells will survive. Myeloma cells will not survive in this medium while the hybrid cells will.