St. Mary's Hosp. Isolate cells 2.
All following steps are at room temperature and in permeabilization buffer.
Treat with permeabilization buffer for 10 minutes. Add unconjugated anti-cytokine monoclonal antibody or isotype control. Incubate for 30 minutes at room temperature. Wash with permeabilization buffer 9. Add FITC anti-mouse or similar 10.