Now open the Options box. For all absorbance runs, it is a good idea to do a radial calibration before the first scan and in most runs, you tell it to Stop XL after the last scan. Acquire intensity data instead of OD data. is used to check lamp intensity and radial calibration using the counterbalanance. Click on OK . Now click on the Method box.
Delayed start is for scanning only and is in minutes.
Once the instrument is set correctly, click on Start Method Scan . Wait 1 hour to allow complete evacuation of the chamber and temperature equilibration of the samples. Hit the Ente r button on the XL console and press Start . The machines are tempermental and Start may have to be pressed more once. If the machine doesn't respond, hit CE and Ente r and then Start .