Air dry and fix in acetone for 5 minutes. Mark circles of 5 mm in diameter on glass slides, using nail varnish. Air dry the slides. Incubate and wash as above. Rinse in distilled water.
Air dry the slides.
Mount the slides in PBS or buffered glycerol and examine for fluorescence. No biological products are in use at present. Acknowledgement Parts of this chapter were taken from or based on the chapters on Trypanosomosis in the 1992 edition of the Manual . The previous chapter was written by P. Finelle. Evaluation du test d'agglutination sur carte pour les trypanosomoses bovines.