Indicators should be on the inside and outside of equipment pack. Expiration dates should be printed on all equipment packs. Color indicators are also included on the outside and inside of equipment sterilized in paper packages. Biological sterilization indicators contain spores that are supplied in closed containers and are included with the instrument being autoclaved. Inability to culture the spores after autoclaving confirms adequate sterilization.
Biological indicators are the most accurate sterilization indicators.
ETO Gas Large Two-Chamber EtO Sterilizer Colorless gas, very toxic and flammable . Requires special equipment Odor similar to ether. Materials must be well aerated after sterilization. Instruments must be dry before immersion. 3 hours exposure time is needed to destroy spores.