E. macularius will accept a variety of foods, and are rarely problem feeders. Crickets are a recommended staple in the captive leopard gecko diet. Wax worms can also be fed. Mealworms may also be offered periodically. It is beneficial to dust food items with a small amount of vitamin and calcium powder prior to feeding.
Calcium supplementation can be especially important for gravid female geckos.
During this period a small, low dish of cuttlebone powder can be placed in the female leopard gecko enclosure. E. macularius are sexually dimorphic with males having a larger and wider head and an overall stockier build. In captivity, breeding can occur at any time of the year by manipulating photoperiod and temperature. Two breeding strategies are commonly employed by herpetoculturists. One is to form a breeding group, or groups, by housing a single male with a number of females within an enclosure of appropriate size.