Rinse 3 or 4 times for 15 min. Please note that these are maximum times for large samples. Smaller samples can use significantly reduced times, e.g. iliac crest biopsies can use steps as short as 2 hours with solution changes on the hour. Small samples can be done in two steps of two hours each. Large samples should be done in two steps of two days each.
All percentages are in weight percent.
Cover the samples for two hours, replace the solution and leave overnight for small samples and two days for large samples. After polymerization, these blocks will react more favorably to the heat and forces generated with the saws and grinding apparatus available today. Use glass, PVC or Teflon embedding molds that can be sealed securely. After covering your sample with embedding solution, draw a vacuum on the mold for 30 minutes. Break vacuum and secure the mold's lid.