Stain in phloxine B at 60 C for 2-3 hours. Rinse in distilled water. Treat in phosphotungstic acid for 1 minute. Rinse in running tap water for 5 minutes. Differential pancreatic islet cell stain.
Cells are pale but useful.
Great stain for goblet cells, paneth cells and microvilli. Dissolve the hematoxylin in the alcohol, and the alum in the water by the aid of heat. Remove each from heat and mix the two solutions. Bring to a boil as rapidly as possible. Remove from heat and add the mercuric oxide slowly.