Title reflects that the location of cap binding site is crucial. Cap works at lots of promoters.
Different promoters have different locations of cap binding sites.
61.5 at lac promoter 41.5 at galp1 promoter 70.5 at MalT promoter. 22 bp near consensus with melR promoter. Use run off transcription assay to map promoters being used. Describe this assay. To see if contacts are similar to know promoter contacts To see if cap is binding even if no activation takes place To see if polymerase promoter complexes are the same, or different.