Rinse in water and let dry between Whatmann sheets. Bands fade in the light, so keep it in your notebook. Mix for 1 minute, then working quickly, dab the blot against Whatmann paper to remove excess liquid while leaving the blot moist, wrap the blot in saran wrap, and expose serially to film in a cassette for 30 seconds, 1.5 min, and 5 minutes. Alkaline phosphatase is a good, sensitive, general purpose detection method. 125I detection is good for quantitation, but isn't any more sensitive, and results can be slow due to long exposure times.
Chemiluminescence can be substantially more sensitive than alk.
There are a couple of chemistries available for this, and it is probably the method of choice where extreme sensitivity is required. As is the case with alk. Remember, if you are using HRP conjugated secondaries to do chemiluminescence, HRP is inhibited by azide, so you don't want to use blotto with azide in it. This style of sealer has an adjustable timer that sets the heating time so that the seals are perfect every time. This style sealer can be purchased from Intermountain Scientific.