Depending on the titer of your donor animal you can calculate what volume of plasma you have to give. The higher the donor plasma titer the lower the volume of plasma that needs to be transfused. Poor transfusions that have had little or no benefits have been from donor plasma that has an unknown concentration. Breeders may use the nearest available animal, draw some blood, prepare plasma, and transfuse the baby. This can often be the case without any outward clinical symptoms.
Blood is drawn from the donor animal into a sterile receptacle.
It is then centrifuged to separate the plasma from the red blood cells. Plasma can be stored frozen for up to a year. It can be thawed and heated for transfusion in warm water. When giving an intraperitoneal transfusion we put the baby on a table with the right side up. With one person holding, triangulate an area in the flank between the front of the back thigh, the last rib and the backbone.