One thousand events were scanned per slide. After electrophoresis, cells with damaged DNA contained less DNA than did the cells in the G 1 phase and therefore appeared in the sub-G 1 area of the histogram. Software statistics provide the percentage of cells in a predefined sub-G 1 area, quantifying the percentage of DNA damage in a cell population. The percentage of cells in the sub-G 1 area is a measure of DNA damage. In addition, the imaging function of the LSC makes it possible to obtain digital images of comets by rescanning the microscope slide.
Comet assay for nuclear DNA damage.
Microscope slide for enhanced analysis of DNA damage using the comet assay. Laser scanning cytometry for comet assay analysis. All rights reserved.