Drain slides on Kimwipes and separate. Place on bottom of plastic container as in step 11. Sandwich slides and draw up solution as in 13. Place slides in plastic container above wet paper towels in total darkness for 1-3 days. Drain, separate slides, rinse in TE to stop reaction.
Color product is soluble.
EtOH 2X histoclear Drain and mount slides in mounting medium. We use Cytoseal 60 from Stephens Scientific. I have listed them below. I have kept tissue like that for over 6 months, with no noticeable loss in signal. This seems to help with the infiltration of the wax into the center of these tissues and does not seem to effect signal.