If your experiment is ruined because you forgot to come in and your colonies overgrew, you may lose points, so don't forget. Keep good records of all of your results, including Klett readings, plate counts, etc. Many experiments rely on seemingly trivial details . All dilutions are to be done in sterile saline unless otherwise instructed. Incubate all liquid cultures in a shaking water bath and incubate plates upside down in the incubator.
Centrifugation is to be done only by the TAs.
On a regular basis remove and discard plates or cultures in the cold room that you no longer need. Each week of classes has FOUR days. Of these the ODD numbered days are the formal lab periods and the EVEN numbered days are the intervening days on which you may have to inoculate cultures, examine plates, etc. Days on which nothing happens are listed to avoid confusion.