An allelism test was performed to determine whether the late-flowering locus from C24, which mapped close to FRI by microsatellite analysis, was a FRI allele. Among 257 testcross progeny, no plants were early flowering, indicating that no recombination occurred between FRI and the late-flowering locus in C24. Thus, the late-flowering locus in C24 is likely to be allelic with FRI . Plants were cold treated for 40 days in short days as described by Lee et al. The averages and standard deviations of the values from 10 plants are presented.
All genotypes exhibit a photoperiod response.
F1 plants from the cross of C24 to Col are later flowering than the C24 parent under all conditions due to the presence of FLC -Col. Microsatellite Analysis of C24 and Col C24 has been assumed to be derived from a Col ecotype. All five Col strains gave identical PCR products for all five microsatellite markers tested. C24 and the Col strains produced the same PCR products for only one marker, nga63. Microsatellite analysis therefore indicates that C24 and Col are not genetically similar.