Future studies will focus on the physical interaction of the multisynthetase complex with other polypeptides and macromolecules, such as elongation factors and ribosomes. This information is intrinsic to a complete understanding of the overall protein biosynthetic process as well as the principles underlying intracellular organization. All of these studies utilize the UMMC Electron Microscopy Facility, which is under the direction of Dr. Norcum. This is based around a LEO912AB transmission electron microscope, which was installed in summer of 1998. The microscope is equipped with a LaB 6 crystalline electron source, integrated Omega energy filter, computerized tilt stage, Oxford cryoholder, and minimum-dose focusing software.
Data collection is via film or a computer-controlled image intensifying camera.
Ancillary instrumentation for computational analysis of biological structures includes an Octane Silicon Graphics computer, AGFA Duoscan flatbed scanner, and Kodak dye sublimation printer. UMMC Electron Microscope Facility. The cytokine portion of p43 occupies a central position within the eukaryotic multisynthetase complex. Norcum, M.T. and Dignam, J.D. 1999. Norcum, M.T. 1999.