See Figure 1 for a sitting drop schematic. In addition to hanging drop and sitting drop vapor diffusion, the Wizard solutions can be used in any kind of macromolecular crystallization technique. Sandwich drop crystallization plates are available from Hampton Research and ICN-Flow. The solubilized biomolecule in its sample suffer can be dialyzed against a larger volume of the Wizard crystallization solution. Microdialysis buttons are recommended for screening by dialysis.
Crystallization occurs by vapor diffusion or liquid-liquid diffusion inside the capillary.
Interpretation of results and optimization Record observations of crystallization trials every one or two days. For 24-well plates, use the supplied crystallization data sheet. The crystallization trials should be viewed under a stereo microscope at 10-100x magnification. Record observations for every condition, even if the crystallization drop remains clear. Crystals suitable for X-ray data collection are generally 0.1 mm or greater in their smallest dimension, and have clean, sharp edges.