The real continuity, through the middle lamella, is scanty and hard to demonstrate. Very satisfactory material for the demonstration of the connecting strands is furnished by the seeds of the Japanese persimmon, Diospyros Kaki. The Philippine Diospyros discolor is even better. Good views of the strands are most abundant in sections cut parallel with the flat surface of the seed. No imbedding is either necessary or desirable.
Cellulose acetate seems to be the best.
Stain in iron-alum haematoxylin. The following schedule for Diospyros Kaki will introduce the method. Fix in formalin alcohol or in glycerin alcohol 4 or 5 days. Chloroform or ether, several hours. Wash in water.