U0126 does not affect vRNA synthesis, transcriptional activity of the viral RDRP or accumulation of viral proteins. Cell lysates were analysed by western blotting for expression of influenza nucleoprotein, M1 and NS1 at different time points after infection. As a loading control, the same membranes were reblotted with a specific antiserum against ERK2. After 25 h cell lysates, normalized for protein content, were assayed for CAT and luciferase activity.
Data are representative of several independent experiments.