In order to obtain best results, it is recommended that each individual user determine their optimum working dilutions by titration assay. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use. Consult the MSDS for information regarding hazards and safe handling practices.
Alcohol fixed rat brain is also suitable.
AEC Mayer's Hematoxylin. Method Deparaffinize and hydrate sections. Develop AEC color for 15 minutes at room temperature as described in the Rabbit ExtrAvidin kit directions. Rinse in H 2 0. Counterstain lightly with Mayer's hematoxylin.