The new technology of CE applies existing theory with new methodology. For example, taking the technique for slab gel separations of proteins and applying it to CE produces precise, quantitative separations. The CE produces very predictable and reproducible results with minimal sample sizes. The theory of CE, three separation methods, and results of these separations are presented here.
Chromatography permits scientists to separate diverse mixtures of closely related compounds.
CE harnesses the power of electophoretic and electroosmotic flow in order to produce separations of samples that consist of only a few nanoliters. The capillary then acts as a salt bridge between the inlet and the outlet vials, continuing the electrical loop. The inner walls of the capillary are lined with a negative charge. This is achieved by the dissociation of functional groups making up the fused-silica surface.1 The walls of the capillay can have any pH above 3. The anions are pulled towards the anode, and conversely, the cations are pulled towards the cathode.