Depending on the study or the object can different adjustments of the camera be chosen. The limiting factor is the wavelength of the light used. This resolution limit does not mean that smaller structures stay invisible. Fluorochromization, for example, allows single molecular complexes to be seen, if the distance between molecules of the same kind is above the resolution limit of the microscope. The appropriate computer program subtracts the content of one image from that recorded seconds or minutes afterwards of the same spot.
All immovable structures vanish and only the moveable ones are depicted.
Cameras with image intensification are used in fluorescence microscopy to document fluorochromes with a fluorescence that is far below that perceivable by the human eye. Again is it possible to use the already mentioned method of vital staining, because far more and far more specific sondes are known today. Since their concentration is smaller than that used in the forties or fifties are the cells damaged less. The technique has only recently been developed, but first results with biological objects exist already.