Add to boiling specially denatured alcohol 3A until a saturated solution is obtained and treat with activated carbon, filtered and crystallize by cooling to room temperature. The third crystallization is washed with diethyl ether and dried in a vacuum desiccator, melting point range 185 deg. The formula and volume required in procedure step V-D is dependent on the adsorptive strength of the Al 2 O 3 . Wash the column with 100 milliliters of chloroform and discard the eluate. Add the Bratton-Marshall reagents.
All fractions show a slight color.
Note the portion containing the first significant increase in pink color. If the color increases in the first portion, the eluting strength of the reagent is too strong. If none of these are suitable, another lot of Al 2 O 3 should be used. If the color increases in the fifth or sixth portion, the eluting strength of the reagent is too weak. L. Hydrochloric acid, 4N. Add two volumes of water to one volume of HCl.