Visualization of gene activity in living cells. Nature Cell Biology 2, 871-878.
Chromatin structure is thought to play a critical role in gene expression.
The ability to directly visualize gene expression in living cells provides a powerful system with which to study the dynamics of nuclear events such as transcription, RNA processing, and DNA repair. Images were taken in living cells every 10 minutes over a 7 hour period. Approximately 2 hours after the turn on of the locus, by the addition of doxycycline, the protein product is detected as a CFP fusion protein that is targeted to peroxisomes. The dynamics of a pre-mRNA splicing factor in living cells. Nature 387, 523-527.