The alternative, shorter protocol described here proved to be an effective way of processing multiple PCR amplification reactions when used in conjunction with Pfu DNA polymerase and the pGEM -T Vector Systems. Vector Systems made it easy to identify recombinants. A-tailing reactions occurred with reduced efficiency if the primers used in the PCR reaction had a 3 terminal A nucleotide. Primers designed with a 3 terminal C, T or G performed with equal efficiency in A-tailing reactions. Vector Systems were also used successfully to clone large PCR products generated using the eLONGase TM enzyme blend.
Acids Res .
Lundberg, K. et al. Kong, H. et al . Cheng, S. et al. DNA Cell Biol. Ordering Information Product Size Cat.