Yeast Genetics Fig. 8. Complementation test procedure for S. cerevisiae . After the streaks have matured, they are replicated onto velveteen at right angles to each other. The streaks on the velveteen are then replicated onto a YPD plate and incubated at 30 degrees for 24 hrs to allow mating at intersections. The resulting diploid colonies are then tested for phenotype.
Colonies displaying wild type phenotypes have mutant genes in different complementation groups.