Each group will need to have the concept of their Lab Practicum approved by Dr. Miesfeld for overall feasibility and scope by Nov. 1, 2000. Scenario A Scenario B Scenario C Scenario D Scenario E 1. Protein has been purified based on a biochemical assay. Express the wild-type and mutant forms of the protein. Determine the molecular mechanism of protein activity.
Developmental gene is isolated from a model organism.
Use GenBank database to find orthologous genes in mammals. Use ESTs to get cDNA and isolate genomic clones. Determine developmental conseqeunces of gene defect in mice. Isolate full-length cDNA and use with ESTs to build contig. Use structure function analyses and cell transfection expts to study function.