Place labeled spin column into collection tube. Add 5 volumes PB Buffer to labeled probe. Transfer to column. Spin 11,000rpm for Eppendorf 5417C for 1 min. Discard flow through.
Discard flow through.
Place column back in collection tube and spin to remove traces of PE Buffer. Transfer column to clean eppendorf tube. Add 50ul EB Buffer. Set one minute. Spin one minute to collect probe.