One Solution Non-Radioactive Cell Proliferation Assays.
Absorbance is directly proportional to the number of living cells in culture in most systems.
The CellTiter 96 and CellTiter 96 AQ ueous Cell Proliferation Assays measure the conversion of a tetrazolium compound into a formazan product by viable cells. Cell Proliferation Assay utilizes MTS rather than MTT as the tetrazolium reagent. During the assay, MTS is converted into a soluble formazan product which does not require the addition of an organic Solubilization Solution as does the MTT-based CellTiter 96 System. One Solution System can be can be read and returned to the incubator, if necessary, for further color development or time course studies. The use of a reference wavelength will reduce background contributed by cell debris, fingerprints and other nonspecific absorbance.