Assignments are performed under close supervision with the intent of enhancing the incumbent's knowledge and skills in order to perform more difficult assignments with greater independence at the next higher grade level and eventually at the full performance level. Typically the research requires the use of preparative centrifugation, gel electrophoresis, recombinant DNA and related molecular biology techniques, mammalian tissue culture and cell suspension, Western Blot, various assays, protein extraction, and handling small rodents. Maintains and grows various bacterial cultures for the preparation of nucleic acids and proteins. Reviews and records results of experiments, compares with expected or past results, and discusses problems and possible causes when problems are encountered. Assists in handling small experimental animals, animal dissection, and preparation of instruments for dissection.
Assists investigator during experimental procedures.
Maintains detailed and accurate records of techniques and procedures employed as a reference point for trouble-shooting when expected results are not achieved, and for incorporation into research papers. Assists the investigator in conducting literature searches. Performs searches to locate research papers related to current research and to verify sources for inclusion into research papers. Performs a variety of laboratory support and administrative duties. Operates, calibrates, and maintains various equipment including microscopes, spectrophotometers, electrophoresis apparatus, fraction collectors, liquid scintillation counters, balances, pH meters, specialized pipets, and centrifuges.