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Cysteine residues are reactive and readily form disulfide bonds.
A peptide map that contains multiple disulfide linked peptides can make analysis and identification of those peptides difficult. At these higher pH if an RCM procedure is not performed disulfide bonds can scramble further complicating the analysis by introducing artifacts. The high pH of the injection mixture will not significantly harm the silica of the reverse phase column because the residence time of the high pH buffer is extremely short. Dithiothreitol Iodoacetic Acid Cysteine S-Carboxymethylated Cysteine . All rights reserved.