Lanes 3-6, reactions contained one component not mixed after thawing, but the assembled components were mixed prior to thermocycling. The Access RT-PCR System provides robust performance over a wide range of reaction parameters, but reactant conditions such as the final magnesium and primer concentration should be optimized for each primer and template combination. The addition of increased amounts of components such as dNTPs and enzyme should be avoided, as this can lead to suboptimal amplification. A Molecular Diagnostics Application. Reverse transcriptase inhibits Taq polymerase activity.
Acids Res .
Promega does not encourage or support the unauthorized or unlicensed use of the PCR process. Use of this product is recommended for persons that either have a license to perform PCR or are not required to obtain a license. 1999 Promega Corporation. All rights reserved.