Therefore, using uncompensable semen will result in a lower pregnancy rate. Research indicates that selection of the male, semen dosage, and site of insemination all positively effect the number of accessory sperm. Bulls vary in the number of accessory sperm per egg, as well as the minimum number of sperm for maximum fertilization. As these traits cannot be predicted from current semen evaluation procedures, they must be gathered from AI non-return data. The data must have sufficient services on a given bull at or below threshold numbers of sperm per dose.
Deep insemination can enhance sperm delivery.
However, site of insemination was found to make only small increases in sperm per egg. So Saacke recommends that very deep insemination be used only when the sperm dose is below threshold, or if sexed semen is being used. Saacke also found that embryonic quality and accessory sperm numbers can be effected by time of insemination. Using varying quality and quantities of semen, he found that the number of accessory sperm was highest when insemination occurred 24 hours after onset of estrus. The quality of embryo, however, was poorest at the time.