RAFT Applications RAFT has been used to select short protein segments that fold in isolation. Pulsed- labeled hydrogen exchange2 as well as equilibrium exchange under both acidic3 and native conditions4 showed that a core of the protein is greatly protected from exchange. By identifying peaks in the RNase HI RAFT profile, a segment, 43-122 ,was identified as a likely autonomous folding unit. Predominantly monomer at CD concentrations. The RAFT scores of all segments of RNase HI are plotted on the left of the figure.
Dark red indicates the highest scores, and dark blue indicates scores approaching zero.
For clarity, segments that have scores less than zero are not shown. Only segments with N termini before their C termini are allowed.