Laboratory emphasis will be on molecular biological techniques utilized in modern research laboratories. Techniques include gene cloning, DNA and protein electrophoresis, protein purification and enzymatic and immunological assays. Chem 220 recommended. Student option grading. Course will teach the fundamentals in tissue culture techniques, subculturing and maintenance of cell lines.
Aseptic technique is emphasized, along with media preparation.
Student option grading. Basic course in the theory and concepts of recombinant DNA techniques. Course will focus on the methods of gene cloning with bacteriophage and plasmid vectors, growth and maintenance of E. coli, extraction and purification of DNA, polymerase chain reaction, southern blots, probe preparation, hybridization methods, and DNA sequencing. The lab will focus on hands on techniques. Student option grading.