This enrichment is added to filtered seawater. Just add dinoflagellates. This enrichment is added to autoclaved natural or artificial seawater. There is also a recipe for artificial seawater, if required. The key to growing dinoflagellates is to give them 12 hr of fluorescent lighting to allow them to perform photosynthesis, and 12 hr of darkness during which they can bioluminesce.
Dinoflagellates only emit bioluminescence during their night phase.
Peak luminescence occurs 2-6 hr into the dark phase. Use a timer to control the lights, with 12 hr of light and 12 hr of dark. 2 hr before you will observe the luminescence. It takes a few days for the dinoflagellates to entrain to a new light cycle.