In general, routine requests take about 48 hours. If purification is required or if you request a sequence containing unusual bases or other non-standard chemistries more time will be needed. The requestor should consult facility personnel at the time unusual orders are placed as to the additional time required. The facility can synthesize oligos incorporating non standard bases, various modifications to either the 5' or 3' end of the oligo, RNA or phosphorothioates. The user is charged the cost of the necessary reagents.
Degenerate bases are routine and there is no additional charge.
We strongly suggest discussing these requests with us before leaving the actual order. If the unusual base or modification is not available through a commercial source, check with us, we have extensive solid phase synthesis capabilities and may be able to help. Purification Most common applications don't require purification of the oligos. The most popular purification method is the trityl-on cartridge. This method exploits the hydrophobic 5' trityl group left at the end of the synthesis and a disposable reverse phase cartridge.