No carbohydrates should be on A or 1 . Golgi and act only on domains in the lumen. In the cytoplasm. The cytoplasmic domains remain in the cytoplasm no matter what organelle membrane the protein is in. Every time a vesicle buds off one organelle and fuses with another, the topology remains the same.
Cytoplasmic domains remain outside the organelle and intra-lumenal domains remain inside.
The SRP should bind to domain 1 . Since the amino end of the peptide is in the cytoplasm, the SP cannot be on the amino end. The SP must correspond to the first transmembrane domain. C-1 . See C-2 below. In gap junctions, all of these are connected to each other.