All biologists express their data on a uniform basis, i.e., in a form that is most universally accepted and understood by all. Two of the most constant units of expression are cell number and DNA content. Often, it is difficult to accurately determine these values.
Acceptable alternatives do exist.
This lab experiment is designed to acquaint you with standard lab procedures for determining the amount of protein present in a solution. The protein content is related to the cell number and can therefore be used to normalize results for day to day or lab to lab comparisons. A protein in solution can be quantitatively assayed by several spectrophotometric methods. Most methods are based on binding of a chromophore to specific amino acids or bonds in the protein. The resulting color development can be detected at some wavelength of visible light.