1982 M.D. University of Rochester 1981 Crystallographic Studies of Drug-Enzyme Complexes. Our laboratory employs the technique of macromolecular crystallography to elucidate molecular structure, and to relate this structure to biological function. Crystallographic methods are used to define the structures of complexes between active ligands and their enzymatic targets. This information is used in conjunction with ancillary modeling and computational techniques to identify, at the molecular level, specific interactions important in stabilizing these complexes. These agents act as general inhibitors of the class of enzymes known as dehydrogenases.
Different inhibitors show marked variations in specificities and affinities for different enzymes.
Structural results at atomic resolution are correlated with biochemical and pharmacological data. The ultimate aim of these studies is to identify conformational features which enhance drug activity. These features can then be preserved in the intelligent design of new analogues. Synthesis, Conformational Analysis, and Biological Activity of C- Thioribonucleosides Related to Tiazofurin. A link between protein structure and enzyme catalyzed hydrogen tunneling.