Cell suspensions were maintained by partial selection of embryogenic cells, which was done by letting the cell suspension settle briefly, followed by discarding the supernatant and replacing the culture medium up to 35 to 40 ml every seven days. Cell suspension growth was measured by embryogenic cell number, packed cell volume and cell fresh and dry matter. No abnormalities in the stomata were observed. The dust did not cause chlorosis in the treated plants. In V. catjung, specific biomagnification of elements like Fe, Ca and Mg was observed.
Different plants behave differently for absorption and biomagnification of elements.
The relationship between hydrogen metabolism, nitrogen assimilation and transport was studied in Glycine max L. cv. USDA DES 122 of Bradyrhizobium japonicum . An increase was observed in the activity of enzymes related to both nitrogen assimilation and ureide synthesis when plants were submitted to shadding. The increase in most observed parameters may explain why plants inoculated with the Hup - mutant were able to compensate for the decrease in total light intensity, resulting in growth rates similar to the control plants. Cultivation of soybean under reduced total light intensity, as in greenhouse or phytotron studies, is likely to show a lack of significant strain effect on sap composition and total fixed nitrogen, and may help to explain why the potential of hydrogenase activity in the association is still a matter of controversy when comparing experiments carried out under different environmental conditions.