CSF and ependyma Ependyma refers to the epithelial lining tissue of the central nervous system. It is the holdover of the original ectodermal lining tissue of the neural tube. During brain development, specific portions of the ependyma along with underlying loose CT, invaginate to form choroid plexus. The CSF is released into the local ventricular spaces, bathes them, and flows out to the spaces surrounding the brain and cord. These are substances known to interfere with cytoskeletal structures.
Colchicine interferes with microtubule assembly-hence arrests chromosome movement in mitosis.
Compare and contrast the following. Both represent results of tissue processing, and can lead to visible effects on the tissue. Improper fixation may result in tissue degeneration. Spreading artifact may occur, especially at the waterbath stage - and lead to artificial gaps between or within tissue layers. Both of these insults would generate observable defects with microscopic scrutiny.