In an early use of RFLP's, Helentjaris and coworkers looked at the segregation of RFLPs in maize. One probe can detect polymorphism at two unlinked loci simultaneously. Segregation of a RFLP in a F 2 population. This figure demonstrates the simple inheritance of a RFLP,9-72s, in a F 1 and F 2 population. In lane 4 are m.w.
Conditions are as noted beofre.
All individuals have at least one A and one B allele. The simplest explanation for this result is that these RFLP's reflect alternative alleles but at more than one locus . A and B each represent discrete loci, because no individuals are seen in which both A or both B alleles are missing. Linkage can be detected between two probes by noting allelic segregation. Table 1 shows two examples of linkage mapping between two linked loci.