The LDA is an immunoassay for the direct detection of Lyme antigen in urine that reacts specifically to rabbit anti- B. burgdorferi antibodies. The urine is processed following the IGeneX, Inc. proprietary sample processing protocol. The antigens present in the processed urine are immobilized onto a membrane in a DOT-BLOT format. The membrane is incubated with anti- B. burgdorferi specific antibodies. The membrane is washed to remove unbound conjugated antibody.
Any positive sample is considered presumptive positive until confirmed by another method.
A negative result indicates no reaction with the above listed antibodies. The RWB can be performed as a confirmation test on any presumptive positive LDA sample. This test is strongly recommended on any presumptive positive LDA when a urine Multiplex PCR was not requested at the same time, or when the urine Multiplex PCR was negative. The RWB is an immunoassay for direct detection and identification of Lyme antigen in urine which specifically react with rabbit anti- B. burgdorferi antibodies. The resolved protein bands are then transferred by electrophoresis to a membrane.