Direct HDL-SL Don't reduce interferences, eliminate them. Today's most advanced homogeneous method incorporates a built-in Interference Clearing System which virtually eliminates triglyceride, hemoglobin, LDL and bilirubin interference. Other direct HDL methods use either antibodies, detergents or polyanions to sequester or block the cholesterol molecules in LDL, VLDL and chylomicrons so that they are unavailable to react with cholesterol esterase and oxidase.
Catalase cleaves hydrogen peroxide to water and oxygen.
The change in reaction conditions exposes HDL particles to the action of cholesterol esterase and oxidase which oxidizes HDL-cholesterol and produces hydrogen peroxide which reacts with a chromogen. Huang Y, Kao J, Tsai K. Evaluation of two homogeneous methods for measuring high density cholesterol. A new direct method for measuring HDL-cholesterol which does not produce any biased results.