Fill up config files for each gel. This step is very important that it is prerequisite for the gel to be analyzed and saved. Fill up list for all gels . Do not delete images when you think that it may be bad. We have computer software to analyze them.
Computer is different from our eyes.
Soak gels in denature solution for 30 min with gentle shaking. Soak gels in neutralization solution for 30 min with gentle shaking. Store the filter dry or start hybridization immediately. Average intensity should be around 200 to 7000 depending on the size of the DNA fragments. The intensity of the marker lanes should be 1000 to 5000.