Treatment of pre-immune and immune sera 1. At the same day of collecting blood inject a boost of your protein 3. Centrifuge at 2,000 g for 15 min at 4oC. Transfer the clear supernatant into new tube. Cool, spin, aliquot as in steps 3, 4. 6. If the titer is low, a third, and fourth injection can be done.
Blood is collected and sera is analyzed see below.