The rabbit is housed for six months. A continuous cell culture system is available for the efficient expression of antibodies in vitro. A ten to fifty-fold increase in antibody concentration is exhibited over traditional cell culture methods.
Additional information is available upon request.
Approximately 5 to 21 days later, depending on the rate of tumor development, the ascites fluid is harvested. Additional flasks of the same cell line wHI reduce the per flask charge. Establishment of Primary Cultures Charges are based on the reagents and complexity of the particular protocol. The sample cell lines are expanded for one week in antibiotic fi-ee medium and then grown on coverslips. The coverslips are stained with Hoechst stain and examined under a fluorescent microscope for mycoplasma Contamination.