The mutant in Lane 8 is characterized by a 4-base deletion at codon 1251, which is easily detected by a significant base shift in the ladder. The mutant in Lane 10 is characterized by a G to T transversion in codon 1250, which is clearly indicated by the appearance of an additional band in the ladder . In all BRCA1 examples, the mutants analyzed were genotypically heterozygous, demonstrating the ability of the BESS T-Scan Kit to accurately detect mutants in such samples. BESS T-Scan analysis of BRCA1 mutations. Reactions were performed as described in the text.
Asterisks indicate sites of mutational change.
Summary The BESS T-Scan Kit is a powerful, new technology for detecting and localizing sequence variations and mutations. The BESS T-Scan method requires minimal bench time, thus expediting the steps between PCR and data acquisition. In addition, the BESS T-Scan system does not require the formation of heteroduplexes, special gels or gel electrophoresis conditions, or extensive reaction optimization. It can be used with either radiolabeled or fluorescently-labeled primers and works well with conventional sequencing equipment. The BESS T-Scan Kit is a novel alternative to currently used mutation screening methods.