Characteristics of human antibody repertoires following active immune responses in vivo. This investigation describes human monoclonal antibodies which in the presence of complement is able to kill meningococci. The variable region sequences of these antibodies are available from GenBank. Human monoclonal antibodies against an epitope on the class 5c outer membrane protein common to many pathogenic strains of Neisseria meningitidis. Antibody reactivities specific for cytomegalovirus glycoprotein B .
Antibody reactivities specific for tetanus toxoid.
Real time analysis of antibody-antigen reaction kinetics. Antibody repertoires developed through in vitro immunization. These technologies permit an analysis of the antibody repertoire found in non-immune individuals. Such repertoires may not necessarily be identical to immune repertoires as shown e.g. by the prefered detection of antibodies against an epitope of HIV-1 gp41 which is not highly immunogenic in vivo. Furthermore, these technologies allow development of antibodies which are not immunogenic in vivo or which are difficult to access because of a lack of suitable immune B cells.