Glycoproteins could be analyzed with high resolution and without fragmentation with the matrix 3-hydroxypicolinic acid, commonly used for oligonucleotide desorption. International Journal of Peptide and Protein Research 45, 312-319. This two-step procedure combined with air oxidation allowed regioselective disulfide bond formation. Analytical Biochemistry 228, 173-177. Purification of synthetic peptides with the aid of reversible chromatographic probes.
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Analytical Biochemistry 224, 451-455. Recipes and tips are given to improve peptide yields when using this popular in situ proteolytic digestion method. Completely drying gel pieces increased peptide recovery about two-fold. Analytical Biochemistry 224, 75-82. Internal Sequences from Proteins Digested in Polyacrylamide Gels.