The deletions are then exploited in several ways to systematically explore the roles of various chromosomal intervals. It is summarized as follows. Irradiation of the targeted ES cell clones is used to induce deletions that remove the selectable marker. These deleted ES cell lines are then injected into blastocysts to generate germline chimeras. We generated several targeted insertions of a tk marker along the proximal t complex, and have isolated numerous cells lines that have deleted the marker gene.
Deletions range in size up to several centiMorgans.
Mice containing several of these deletions have been transmitted through the germline. The data are summarized in the spreadsheet below. This entails crossing progeny of ENU-mutagenized males to mice bearing deletions. Nested deletion breakpoints as described above can then be used to rapidly localize new mutations. We have already generated a series f deletion complexes across the proximal region of Chr 5.