We will supply common primers such as T7, T3, Sp6, M13 forward, and M13 reverse. The CEQ2000 can handle single or double stranded DNA templates or PCR fragments. Please note the mass of DNA to be supplied for different types of sample - the amounts indicated below and on the sample sheet give the best results. Also, the DNA must be column purified for best results. All sections of the form must be completed as this helps us tailor the sequencing reactions to your particular sample.
Crucial information is the length of the DNA molecule .
We can also provide free PC software to view and edit sequences prior to export. We are currently running this service at cost so each sample is a bargain at 3.00 per reaction. This cost will be reviewed in the summer of 2001. The cost will be recharged to a KCL account code and a code must be supplied before use.