Alternatively, this entire web page including the order form may be printed directly. Samples must be prepared using the following guidelines to ensure that the reaction proceeds as it should. Inappropriately prepared samples will be charged the full amount, regardless of the result. All non-labelled sequencing primers must be supplied by the client.
Alkaline lysis is acceptable but is at the bottom of the list.
If this method is chosen, two chloroform-phenol extractions and an ethanol precipitation step should be performed. Promega Wizard preps do not consistently produce good results. It is essential that the DNA prep be extremely clean since the chemistry used in the automatic sequencing protocol is significantly more sensitive than standard 'manual' sequencing techniques. A spectrophotometer should be used to determine DNA and primer concentrations. The labelled dATP is best placed immediately following the ultimate 3' base of the oligo.