Fluorescence is an intrinsic, non-invasive and highly sensitive probe of biological reactions. In addition, fluorescence of chromophores is used to monitor excitation energy migration and transfer. In these pages, we will examine the principles and the methods for measuring the lifetime, quantum yields, polarization, action and emission spectra of fluorescence in several biological systems. Two additional meetings will be added during the course.
Dates are yet to be determined.
References for lecture topics provided under the date's link. Fluorescence Assay in Biology and Medicine. Academic Press D.M. Hercules, Ed. Original is in Russian. With applications to photochemistry and analytical chemistry.