Forms for requesting the production of cell cultures and for requesting Mycoplasma testing services are available for download and printing. The Cell Culture Core provides Cell Banking to Investigators. The Core has the capacity to freeze and store 10,000 vials. As part of our quality control program we have a Mycoplasma and quarantine facility. The core will test for and eradicate mycoplasma from cell cultures.
All cell culture is performed in a sterile environment employing aseptic techniques.
We routinely perform a direct and an indirect method of testing for Mycoplasma. This method requires the use of enriched mycoplasmal media and controlled conditions. This method does not detect all strains but with the appropriate controls this works very well. Direct culturing is a reliable test and takes 2 weeks to complete. We routinely use an Eliza kit from Boehringer Mannheim.