However, the staining time with SYBR green of the SeaKem agarose gels should be doubled. The loading well dimensions are critical because, along with the loading volumes and molar concentrations, they determine the spatial concentrations of DNA in the bands. The fluorescent response for a SYBR green-stained band will saturate when the spatial concentration of DNA in the band is too high. The thickness of the gel also affects image quality.
Bands appear fuzzier on thicker gels because the gel itself is a light-scattering medium.
DNA loading The amounts loaded must be tightly controlled. Allow at least 2 hours at 37 degree C or overnight at 4 degree C for pelletized DNA to dissolve after resuspension - before you measure the concentration. Both the volume and the amount of DNA loaded in each well are critical. The two variables that most affect running times are temperature and voltage gradient. Do not just measure the voltage across the gel box and divide by the box length.