In Florida, it is found from the central peninsula northwest into the panhandle. Outside of Florida, it is found along the Atlantic coast to Virginia. Uncommon, found in cypress strands, sloughs, sphagnum bogs, swamps, creeks, and streams. The glossy crayfish snake is aquatic. It is also occasionally found burrowed beneath logs and crossing roads after heavy rains.
Adults feed primarily on crayfish, fishes, frogs, and salamanders.
Juveniles may also feed on aquatic invertebrates such as dragonfly larvae. It is live-bearing, with breeding occuring in the spring. The young are born in the summer months.