As the technology of genetics has grown so has its uses. Genes and their protein products are the two most important resources to figuring out biology. By isolating genes and their protein product, function of the protein and gene can be demonstrated. Isolating a gene, inserting it into specific plasmids and cloning it into bacteria with protein making machinery produces a protein specific for the gene of interest. The protein can then be used to form antibodies.
Antibodies are excellent diagnostic tools as they are monospecific for a protein.
This process allows one to go from a gene with no known function to a known function for its protein product. It allows specific hunting with antibodies which is a powerful way to see the location of the protein of interest. Antibodies for each were produced but the antibodies cross reacted. In order to compare the two gene products we need antibodies that won t cross react. The Bold portion is the region that was amplified and expressed for antibodies.