Characterization of native andmutant proteins involve enzymological techniques, such as spectral titrations and rapid reaction kinetic measurements, protein chemistry methodology to define cysteine content and reactivity, and structural work, which includes protein crystallization and analytical ultracentrifugation. Alkyl hydroperoxide reductase and thiol-specific antioxidant define a large family of antioxidant enzymes. Flavin-dependent alkyl hydroperoxide reductase from Salmonella typhimurium . Purification and enzymatic activities of overexpressed AhpF and AhpC proteins. Flavin-dependent alkyl hydroperoxide reductase from Salmonella typhimurium .
Cystine disulfides involved in catalysis of peroxide reduction.