Evaluate the direction of the peaks. If negative, change the polarity switch on the detector. Adjust the height of these peaks by changing the AUFS setting on the detector. Evaluate the separation of the peaks. If insufficient for proper quantitation, consult with your instructor regarding the best course of action to take.
Analysis Inject the individual standards to unambiguously identify the peaks.
Inject the mixed standards, starting with the largest concentration. Each of the mixed standards should be injected, in decreasing order, until the series is complete. Then, repeat the injection series two more times. Evaluate the reproducibility of peak heights. The plots should be linear.