Be sure to mix the culture to ensure cells are in uniform suspension before withdrawal . Use 10 ul of culture to make a wet mount on labeled slides at each time point. This will help you plan for any dilution needed to make cell counts using the hemacytometer. Analysis of Cell Cycle Stages during Logarithmic Phase Growth Assess the percentage of yeast cells in G1, S, G2, and M using the following morphological criteria. Phase Contrast Micrographs.
Arrows indicate buds.