Background Prior to 1960, when Moorehead and Nowell described the use of Giemsa in their chromosome preparations, conventional cytologic stains such as acetoorcein, acetocarmine, gentian violet, hematoxylin, Leishman's, Wright's, and Feulgen stains were used to stain chromosomes. Rinse well in buffer. If stain is too intense, wash longer in buffer. If it is too weak, restain the slides. Blot dry with bibulous paper.
Air dry completely.
Rinse in xylene. Mount in neutral mounting medium. In The ACT Cytogenetics Laboratory Manual, Second Edition , edited by M. J. Barch. The Association of Cytogenetic Technologists, Raven Press, Ltd., New York, 1991.