The ligation is incubated at 15 C for 3 hours to overnight. Several methods can be used to increase the number of clones obtained. Caution should be exercised to avoid overexposing DNA to UV light if viewing PCR products in an agarose gel prior to gel purification. Additionally, the use of SOC medium instead of LB after heat shock may enhance system performance. In Griffin, H.G. and Griffin, A.M., eds.
Acids Res .
Protocols and Applications Guide , Promega Corporation, 53. Murray, E. et al . All Rights Reserved. Wizard is a trademark of Promega Corporation.