Add 1 ml of extraction buffer to the tube containing the mycelial powder and mix gently. Let stand at room temperature. for 20 min. Transfer the homogenate to a microfuge tube and centrifuge in a microfuge for 5 min. Let this stand at room temperature. Ten samples can be filtered simultaneously by using two flasks from one aspirator with a Y-junction.
Commercial manifolds are also available.
Place each column into a 15 ml conical tube with the tip of the column resting inside a 0.5 ml microfuge tube at the bottom of the 15 ml tube. Stable for approximately 6 months at room temperature. The quality of the DNA is high, with the size averaging greater than 20 kb. The DNA is more dilute than in typical extraction procedures, but may easily be concentrated by lyophilizing or by precipitation. After transfer to nylon membrane, the DNAs were probed with the transforming DNA fragment that was labeled by the random hexamer priming method.