If DNA was not good enough, repeat the procedure. Boil 5 min and transfer to ice before use.
Air dry the pellets.
Boil 3-5 min aand keep on ice immediately. Rinse gel with dH 2 O for couple of min to remove urea. A processed sample image is available for downloading . If using image 3.x, the 8-bit tiff file from ImageQuant can be used directly. Refer to the Sanger Center for more information and a step by step tutorial.