The beads are used to adjust instrument settings, set fluorescence compensation, and check instrument sensitivity.
Daily use is recommended for monitoring instrument performance over time.
Refer to the information appropriate to the instrument setup you are performing. Both scatter and fluorescent light signals are detected. However, each fluorochrome emits light over a range of wavelengths when excited by the laser beam. Fluorescence sensitivity is determined by measuring the mean channel separation between the signal of the labeled beads and the unlabeled beads. A minimum channel separation must be met for the scatter and fluorescence parameters.