Lymphoblastoid cell lines obtained from the Coriell Cell Repositories were established by Epstein-Barr Virus transformation of peripheral blood mononuclear cells using phytohemagluttinin as a mitogen.
All cells lines are free of bacterial, fungal or mycoplasma contamination.
Lymphoblast cultures are shipped in T25 tissue culture flasks which have been filled to capacity with carbon dioxide-equilibrated medium to provide sufficient nutrients for extended transport times. Flasks should be incubated in an upright position with vented or loose caps. Lymphoblastoid cell lines grow in suspension culture with cells clumped in loose aggregates. These aggregates can be dissociated by gently agitating the culture or by gentle trituration with a pipet. In three to four days the culture is either refed with fresh medium or split again depending upon how fast the particular line grows and the desired number of cells.