Please read the disclaimer and the introductory pages . Your submissions and comments are always welcome. The pages have been assembled by Brendan Babb at the department of Medical Microbiology, University of Cape Town . This is the preferred Solution III for plasmid preparation .
Component stock conc final conc.
10x Component stock conc final conc. C Generally manufacturers supply a ligation buffer, but a tried and tested buffer is given below. 10x Component stock conc final conc. DMSO transformation TSB Component stock conc final conc. If ready made broth is available, one can simply add the PEG, DMSO and Mg salts to it - the level of dilution is not significant.