Methods used for the cloning of genes from large cloned DNA segments such as cDNA selection or exon trapping are compared to gene-identification computer algorithms used for searching genomic DNA sequence databases. Bioinformatics sessions emphasizing end-user applications, comparative genomic analysis and database mining are incorporated into multiple problem solving sessions. Recombinational cloning of disease-related loci using BACs and YACs is used as a means to circumvent conventional library construction and cloning techniques. Studies in mutational analysis are also included. The laboratory-based component is supplemented by lectures from prominent investigators that have pioneered the development and application of techniques utilized in the course.
All participants present a synopsis of their work.
These brief presentations serve as a means to discuss positional cloning strategies in the context of real research projects. Due to conservation of genomic sequence across a diversity of species, investigators engaged in projects outside mainstream sequencing projects are also encouraged to apply.