Our studies on yeast glycoprotein synthesis use a combination of classical enzymology, genetics and molecular biology to try to answer the difficult questions remaining in the N-linked glycoprotein synthesis field. My lab is focusing on a mutant cell line called sec59 which is defective in protein secretion and has abnormally low levels of dolichyl phosphate, a key cofactor required for asparagine-linked glycoprotein synthesis. We have found that the sec59 gene encodes the enzyme dolichol kinase and are using the sec59 mutant, along with suppressor mutations of it to explore novel areas of the glycoprotein synthetic pathway. Specifically we are trying to discover the reasons for the lethality seen in mutants such as alg1 and alg7 that effect early steps in the glycoprotein biosynthetic pathway.
Cells are deficient in dolichol kinase activity.
USA 89, 7013-7016. Meeting of basic and clinical research. J. Florida Med. Intermediates in the folic acid biosynthetic pathway are incorporated into molybdopterin in the yeast, Pichia canadensis. To request information, please visit Applying or Contact Us .