After sorting the data by edited status or reaction conditions, we have generated some preliminary conclusions. Foremost is the effect of editing. Manual editing of the data clearly improves the sequence accuracy. Most of the data returned was obtained using ABI instruments and software and is reflective of that system's strengths and weaknesses. This test sequence included sequence stretches that are extremely difficult for the TaqFS dye-terminator system to read correctly.
Conclusions regarding the effects of altered reaction conditions are preliminary.
The accuracy of non-edited data is improved by adding DMSO to sequencing reactions. However, the effectiveness of altered thermocycling conditions is unclear because different laboratories use different protocols. The Committee hopes this presentation of preliminary results from this study will help member laboratories assess methods for sequencing GC-rich templates. BioTechniques 21, 678. 2. Nature 386, 366-402.