PCR-based methods offer the advantages of speed and convenience for simple substitutions. These methods, however, typically are dependent on the properties of the thermostable DNA polymerases being used. Site-directed mutagenesis possesses much power as a research tool and is the basis for a wealth of the molecular biology experimentation in protein structure and function studies today. Understanding what types of mutations are possible and what resources are necessary is an important first step in exploiting site-directed mutagenesis. Capson, T.L. et al .
Acids Res .
Acids Res . Acids Res . Horton, R.M. et al . 1997 Promega Corporation. All Rights Reserved.