Cool the solution to 60 - 70 C and add dye if desired. Pour the molten Super Agarose into an electrophoretic tray, position the comb in the molten gel and allow it to harden by storing at room temperature for 1 - 1.5 hr. 2. Mix DNA or RNA samples with a glycerol-dye loading solution and load them into the application slots with the gel placed in the electrophoretic apparatus and submerged in the buffer. Alternatively, first load samples into the application slots and then place the tray with gel into the electrophoresis apparatus filled with buffer. For RNA denaturing gels, perform denaturation with either formaldehyde or glyoxal and load the denatured RNA samples into the application slots.
Different applications call for varying agarose concentrations.
When working with multiple samples, load samples into the application slots and start electrophoresis as quickly as possible. Delays during sample application permit premature diffusion of RNA and DNA molecules into the gel. To accelerate uniform entrance of DNA and RNA into the gel, run the gel at 100 V for the first 2 - 3 minutes of electrophoresis. Na 2 EDTA . Na 2 EDTA .