Store at 2 to 8 C until analyzed. Analyze on a FACS brand flow cytometer. Mix samples thoroughly before acquisition. Each research laboratory should establish normal ranges using its own test conditions. Avoid excessive centrifuge speeds and vigorous mixing of cells.
Cell damage can cause non-specific staining, cell clumping, and excessive debris.
The amounts of antibodies recommended for use are based on studies of normal human blood. Donors with nucleated red blood cells can show incomplete lysis of the red blood cell population. An indicates this product is an analyte specific reagent. Available in the U.S. only. An indicates this product is for in vitro diagnostic use.