In each case the two colony phenotypes were present in equal numbers, suggesting that insertion into the plasmid in different orientations results in different expression levels of the lac Z gene. In some cases the insertion of a DNA fragment in one or both orientations may not completely interrupt expression of the plasmid lac Z sequences. The presence of a large number of blue colonies after transformation with a ligation mix may be indicative that some of the blue colonies contain recombinant plasmids. Easy Vector Systems allows investigators flexibility in determining the time and temperature of ligation reaction incubation. The 2X Rapid Ligation Buffer can be used to clone various sized PCR products from different templates into the pGEM -T Vectors.
Acids Res .
Ordering Information Product Size Cat. Easy Vector Systems II include JM109 Competent Cells, High Efficiency. No. 5,487,993 and European Pat. The PCR process is covered by patents issued and applicable in certain countries. Promega does not encourage or support the unauthorized or unlicensed use of the PCR process.