Web Movie 1. Activated T cells migrating on ICAM-1. Locomotory PBTLs are characterized by a remarkably higher degree of polarity compared to the cell failing to undergo net body translocation. This locomotion type commences after initial cell spreading and firm adhesion and thereby is likely to provide adequate resistance to externally applied shear forces, e.g. such as vascular flow.
Cells move at apparently random directions due to the absence of exogenous chemotactic stimulation.
Web Movie 2. Web Movie 3. None of the cells undergo significant translocation over a 3 h observation period. However, the cells remain adherent and are capable of producing multiple short-living pseudopodial structures. Frames collected over 180 min with a 40 min break during the observation period.