Set other options by clicking one of the four buttons at the bottom left of the nonlinear regression parameters dialog. Clicking these buttons will bring up a second dialog. Prism automatically supplies initial values for all variables, so you rarely have to think about them. Press this button to inspect and alter the initial values. See Initial values with Prism .
Constants Fix the value of one or more parameters in the equation to a constant value.
See Fixing parameters to constant values . Method Choose how Prism weights data, handles replicates, and other options. See Method options with Prism Output Configure the output by choosing the beginning and end of the curve, the number of significant digits, and more. See Output options with Prism . Press OK to fit the curve.