On the creek bank, empty contents of Surber into large bucket. Rinse Surber and empty into bucket until all animals are removed. Great care should be taken in this step to collect and preserve all organisms from the Surber sampler as well as from the rocks and water in the bucket. Use of a magnifying glass and tweezers is essential. Rinse bucket through sieve to remove water from sample.
Archive sample Use spatula to move sample from sieve into a plastic vial.
Fill vial to the top with isopropyl alcohol. Put label on inside of vial with name of sampler, date, location, and replicate number. Write location and date on top of vial lid. Place vial in a Ziploc bag labeled with the same information. Collect replicate samples Return to the location of the first sample, walk upstream and collect another sample of invertebrates.