Area above 0.00 possibly corresponds to hydrophobicity when the peak reaches the 2.00 range. This particular hydropathy plot shows that pyruvate kinase has many possible integral membrane domains and could be an integral membrane protein. This is consistent with Pyruvate kinase's role in cellular respiration. The key decoding the colors are listed above the figure. Sequence alignment of five different species' Pyruvate Kinase proteins.
Black boxes indicate similarity between sequences.
Dashed lines are included as spacers to increase similarities between the proteins. Finally, using the MacDNAsis program, I generated a phylogenetic tree illustrating the evolutionary relationship between the five proteins. The similarity between the proteins dropped off quickly between human, mouse and rat and the fruitfly and yeast were by far the least related. HERE Return to the Molecular Biology Home Page , or Immunology Home Page . Copyright 2000 Department of Biology, Davidson College, Davidson, NC 28036.