These holes must NOT be covered during operation. In the event of an accidental release of aerosols from a broken tube, these aerosols will be vented by the centrifuge into the laboratory. Materials presenting a chemical or biological hazard in aerosolized form should not be processed in a centrifuge of this type unless secured in a sealed rotor or basket, or unless the centrifuge is contained within a functioning chemical fume hood or biological safety cabinet, as appropriate. Extreme care should be taken to ensure that the centrifuge is properly balanced, and that tubes are free from visible defects prior to use. DO NOT centrifuge uncovered specimens.
Centrifugation creates a vacuum and volatilizes liquids causing aerosolization.
During centrifugation of samples likely to contain pathogens transmitted by aerosols, such as Mycobacterium sp. or Neisseria meningitidis , centrifuge tubes must be in sealed baskets. Extreme care should be taken to ensure that the centrifuge is properly balanced, and that tubes are free from visible defects prior to use. Tube Stoppers Removing the stopper from a tube may produce aerosols or splatter. The stopper should be twisted gently during removal. Remove stoppers behind a bench shield or while wearing a biohazard face shield will provide protection.