The techniques which we employ to study the above areas include tissue culture, microspectrofluorometry, HPLC, gas chromatography-mass spectrometry and in vivo measurement of brain blood flow. These methods allow us to pursue our objectives, whether they be at the biochemical, cellular or in vivo level. Our efforts are currently supported by two NIH research grants and an NIH training grant. Traumatic injury of cortical neurons causes changes in intracellular calcium stores and capacitative calcium influx. J Biol Chem.
Astrocytes generate isoprostanes in response to trauma or oxygen radicals.
Stretch-induced injury alters mitochondrial membrane potential and cellular ATP in cultured astrocytes and neurons. Alterations in calcium-mediated signal transduction after traumatic injury of cortical neurons. Enhancement of AMPA-mediated current after traumatic injury in cortical neurons. J Biol Chem. J.T. Weber, K.A. Willoughby and E.F. Ellis.