In all of them one or more copies of the ura3 gene of S. macrospora are detectable, while the internal pyrA gene of the recipient strain is not labeled since hybridizations were performed under high stringency conditions. Our data prove that the isolated ura3 gene of S. macrospora is functional and can be used as a selection marker in transformation experiments. Comparison of amino acid sequences from OMPD genes from different eukaryotes. In the cases of Arabidopsis thaliana and Bos tauris, only the OMPD domains of the polypeptides were used. Asterisks below the sequence indicate amino acid residues, which are conserved in all sequences.
Dashes represent gaps introduced to obtain the best alignment.
Chromosomal mapping of the ura3 gene from S. macrospora . Roman numerals indicate positions of S. macrospora chromosomes. On the right, sizes of marker chromosomes are given in Mb. The filter bound chromosomal DNA was probed with a DNA fragment representing the open reading frame and adjacent regions of the ura3 gene. Autoradiograph from a Southern hybridization experiment with DNA from A. niger transformants.