Twelve transmembrane domains of the rat dopamine transporter are shown below. All twelve rDAT TMDs in one figure for comparison of conserved regions is also available.
Black circles are residues conserved only between the monoamine transporters.
Red letters denote mutated residues in helical net figures which affected some aspect of binding or transport of substrates or inhibitors. The results may indicate that the two tyramines are part of a hydrophobic pocket.