In another experiment we have analyzed products synthesized in all wells of the microtiterplate by HPLC and mass spectroscopy. We have not found any traces of contamination by liquid or solid transfer between wells in our model experiments. Figure 6 Figure 9 shows HPLC traces of products synthesized in adjacent wells and Figure 7 shows the mass spectra of the products from the same wells. Later we have built the dedicated centrifuge with 8 positions for microtiter plates. This centrifuge is driven from the computer and all centrifugation parameters can be flexibly changed.
Centrifuge can be.
Inclusion of the pipetting system allows us to perform the whole synthesis in completely automatic regimen. Figure 10 Figure 11 shows the view of the first centrifuge prototype integrated with Packard Multiprobe 104 liquid distribution system for the delivery of individual building blocks and reagents. Figure 11 shows the detail of the instrument deck. This compact system can be easily enclosed in inert atmosphere. Since the goal of this instrument is its affordability in the laboratories with any size budget, we decided to design the system, which can be operated semi-manually and later upgraded to fully automated machine.