TRP should be kept in the fridge and cover bottle with foil. Avoid a dark brown color. Usually 25 min in the autoclave is sufficient and remove medium soon after pressure is down.
Autoclaves stay hot if door is kept closed.
Amino acid powder can be sterilized by boiling it in 2xSD for 2 min in microwave. 300 ml of agar takes approx. 2 min to melt in microwave. Agar can be over-cooked and weakened. Do not autoclave longer than 30 min if possible.