The host animals are hyperimmunized with highly purified immunogens. Any contaminating antibodies are removed by solid phase immunosorbents and other adsorption techniques. All antisera have a microbial growth inhibitor added and are filtered through a 0.2 micron filter. IgG fractions are available upon request. Affinity Purified Antibodies Affinity purified antibodies are isolated from antiserum by affinity chromatography.
Affinity purification provides a high degree of specific immunoreactivity.
Affinity purified antibodies are predominantly of the IgG isotypes but depending upon the particular source, may also contain small amounts of the other isotypes. Each vial of affinity purified antibody contains 1 mg of active antibody in a phosphate buffer with microbial growth inhibitor and is filtered through a 0.2 micron filter. Each vial contains 1 mg of active antibody in a phosphate buffer with a stabilizer and a microbial growth inhibitor. Enzyme Conjugates Affinity purified antibodies are available conjugated to the enzymes horseradish peroxidase or alkaline phosphatase. Each vial of peroxidase conjugate contains 1 mg active antibody in a stabilizing buffer with a microbial growth inhibitor.