The female and male scorpions live in separate places and only come together for a very short period of time to mate. During mating, the courtship resembles a dance. The female will then pick up the spermatophore. Adult females are found under and among the rocks and beneath dead standing trees. Males and immature scorpions inhabit leaf litter and live under the bark of dead trees.
Birth occurs in deep crevices and cavities under rocks or in decayed logs or stumps.
The gestation period is one year and litters may contain as many as 40 young. They are white and resemble adult scorpions. The Plain Eastern Stripeless Scorpion usually gives birth in late summer or early fall. After birth, the young climb onto the back of their mother where they stay for about a week before shedding the outer layer. This is called molting.