Place sterile filter paper on top of PDA media in a petri dish. The moisture from the PDA thus saturates the filter paper. Place a small plug of anthracnose isolate on top of filter paper. Put petri dish containing filter paper inside desiccator for 24-48 hours. The filter paper at this point has probably rolled up.
All cultures are single spored upon initial collection.
The culture is then transferred to the outer edge of agar not covered with filter paper, one piece on each side of the plate. In this way, you can tell if the culture you transferred is contaminated. It will also grow if placed directly on the filter paper, but if your filter paper is contaminated, you will not be able to retransfer it. After 1-3 weeks, when the culture has covered the filter paper from both sides and is sporulating, the disk can be peeled off using sterile forceps. Leave this petri dish in the laminar flow hood with the lid on 3-4 days to dry completely.