The reagents are stable at ambient temperatures for the periods of time expected in normal shipping procedures, but the package should be refrigerated upon arrival. The package contains sufficient materials for dissociation of five separate tissue aliquots of up to 0.3 - 0.4 cm3 each. For larger tissue samples prepare proportionately larger volumes of reagents at each step and combine them in the same ratio as described in the protocol. Aliquots of this vial are used to reconstitute other vials and to prepare dilute inhibitor solution. The material is 0.22 micron membrane filtered and lyophilized in autoclaved vials.
Brief incubation is needed to insure full solubility and activity.
This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. Avoid vigorous mixing. Vial 4 Ovomucoid protease inhibitor with bovine serum albumin, one vial per package. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. The inner rubber stopper can be discarded after reconstitution.