Add 10-15 grains of boiled wheat to 175 mls of dH2O. 2. Inoculate with 20 ml from an excellent existing seed culture dish or with a sample from the commercial inoculant. Grow for 7 to 12 days before using. To each bowl add 8-9 grains of of boiled wheat and 8 ml from a paramecia seed culture. 4.After 10-14 days, the paramecia are ready to feed to the fish larvae.
Cultures remain useable and healthy for a month or more.
Fill plastic mouse cage or equivalent with 2 liters of system water. Cover and store in a warm, well-lit place. The covered mouse cages may be stacked three layers high with the highest layer closest to a warm light. It will be ready to feed first. The cultures are then ready to use in four days and are depleted after two weeks.