Protein Extraction Samples were taken at different developmental stages as indicated in Table 1 and Figure 1. Proteins were analysed in bulked tissue samples which contained both meristematic and corm tissues in unknown proportions. The supernatant contained predominately soluble proteins. The proteins in the phenolic phase were washed with 5 volumes Figure 1. Different developmental stages of tuberose corm apices.
Arrow indicates the early stage of flower primordium.
Effects of different photoperiods on floral initiation and development in tuberose. Early stage of floral initiation. Chang et al. Daylength and flowering in tuberose of 0.1 M ammonium acetate in methanol at -20 C overnight. After centrifugation, the pellets were washed with 0.1 M ammonium acetate in methanol and centrifuged again at 10,000 g at 4 C for 5 min.