Aslanidis et al. Jones et al. The methods can be performed using only one primer and are also useful in cloning protocols and for sequencing large DNAs. Subsequent multiple cycles of annealing primers, extending the primers, and denaturing duplex products are usually performed so as to achieve the desired degree of amplification. Sequencing of large DNAs is performed using multiple rounds of DNA amplification, each round employing a primer homologous with a primer target site in the sequence of interest previously amplified.
Cloning is facilitated by including a replication origin and selectable marker in the adapters.
NatL Acad Sci. Jayaraman, et al. Shuldiner et al. Horton et al. This is a continuation of application Ser.