The applicability of these equations to laboratory situations varies as additional components in the hybridization environment are altered. It is best to consider these predictions guidelines, since they may vary for particular sequences from empirically derived determinations. Background Problems Moderate background on filter hybridizations is common. Howley, P.M., Israel, M.F., Law, M.F., and Martin, M.A. J. Biol. Wallace,R.B., Shaffer,J., Murphy,R.F., Banner,J., Hirose,T., and Itakura,K. Nucl.
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