Man's shame and fear towards God. Before the fall, Adam and Eve walked and talked with God. To us, how lucky they were. After they ate, they could not stand the sight of God, and felt ashamed in confronting God. To those who may take the Garden of Eden less literally than others, you can still see in yourself and others the shame of being around God when we know we've sinned.
All mankind is now sinners.
The Earth is also poisoned by sin. God's wrath against sin. Mankind was kicked out of the Garden of Eden by God for sinning, and was prevented from returning. God hates sin, and it was a while before one's sins could be dealt with in the Mosaic laws. Sin is an act against God, in breaking his rules as to how we are to live.