Take sample material from each cell with an appropriate scoop to the bottom of the cell. If the results of the second analysis are lower than specifications and when averaged with the first analysis produces a result within the specifications, then the lot is considered acceptable. On the other hand, if the second analysis is still higher than the specifications but lower than the first analysis, a third analysis can be performed on the third sample. If the average of the three analyses is within specifications, the lot is still considered acceptable. Otherwise, it is rejected.
Aflatoxin is extracted by aqueous methanol followed by clarification with zinc acetate.
Aflatoxin is then trapped in the florisil layer in a minicolumn. This method only gives results indicating higher or lower amounts than the standard. The whole process takes only 10 minutes. This is a rapid method, but is not recognised by standard organisations. Aflatoxin is extracted by aqueous acetone followed by clarification with ferric gel.