Biol., Amsterdam, Poster 37. Carlson, J.E., L.K. Tulsieram, J.C. Glaubitz, V.W.K. Luk, C. Kauffeldt, and R. Rutledge. Segregation of random amplified DNA markers in F1 progeny of conifers. Chowdhury, M.K.U. and J.K. Vasil. Molecular analysis of plants regenerated from embryogenic cultures of hybrid Figure 1.
Arrow indicates the size of the fragments as compared with markers.
In our study, the amplified products exhibited monomorphisms among all the in vitro plants and were similar to those from control plants. The method is simple and the results are reproducible. Because only micro-amounts of material are necessary, this approach can be used to assess tissue at several stages of in vitro culture. Furthermore, the genome is most probably randomly sampled without the influence of ontogeny. However, only major fragments genetically characterized through segregation analysis should be used as markers.