Those that do survive are killed. Sequoia states that the animals used in the LD50 test are generally force-fed substances that can range from toothpaste to drain cleaner to pesticides. These substances are administered through injection or forced inhalation, or they are placed directly on the animal's skin. According to the March 1995 edition of the journal PETA , there are many reasons why tests such as the LD50 are inaccurate. Dr. Michael W. Fox states that conditions for the animals in the laboratory are deplorable.
Cages are small, and in most cases, the animal barely has room to move.
These animals also have little or no contact with humans and other animals. Many of the animal's basic needs for clean air and adequate nutrition are ignored. These conditions affect the animal's behavior. This makes any results of research done on the animals extremely questionable. The PETA journal published in March 1995 states amazingly that tests like the Draize test and the LD50 test are not required by law.