In the latter, more energy is used for gonad development during the maturation. Abstract Using DAPI stain method, the nuclear developmental processes of the fertilised eggs of scallop Chlamys farreri was observed and described under fluorescence microscope in this study. Male and female scallops obtained from Qingdao suburban scallop farms were induced to spawn in plastic bucket filled with sea water at 18 o C, respectively. After spawning, the eggs and sperms were mixed with proper proportion. I, until activated by seawater.
Activation makes the meiosis continue.
15min after fertilization, a few fertilized eggs extruded the first polar body. During this period, the incorporated sperm nucleus decondensed and enlarged simultaneously. 35min after fertilization, the second polar body could be observed on surface of the eggs. During this period, there were no obvious changes were observed on the sperm nucleus. Formation of the male and female pronucleus.