The diagnostic and prognostic value of DNA ploidy analysis has been demonstrated in a variety of solid tumors. The capability to perform DNA ploidy studies on paraffin-embedded tissue has led to its widespread use in the clinical setting. The ideal specimen for DNA content analysis of archival material would be one that preserved tissue architecture without generating sectioning artifact. However, the process of nuclear extraction causes complete loss of tissue architecture. DNA content analysis of paraffin-extracted nuclei from a breast adenocarcinoma.
Architecture is especially important in biopsies with small foci of tumor.
This produces a searchable plot of the histologic section. DNA content analysis of prostate needle biopsy. Sample Preparation Nuclear Extraction . Three 50 micrometer sections are cut from the tissue block for nuclear extraction. Tissue sections are deparaffinized and rehydrated in xylene and a series of decreasing ethanol to water using a tissue processor.