Additionally, a test on the toxicity of the effluent to aquatic life must be conducted and reported quarterly. The operator fills out a monthly discharge report and submits it to the Tennessee Division of Water Pollution Control. The data is keyed into a national database at EPA and violations are noted. Yes, but not necessarily every month. Sometimes the notice is issued quarterly.
Bacteria tend to grow themselves.
At least 90 days in holding tanks. HVUD has separate sanitary and storm sewer systems. However, any stormwater that infiltrates into the sanitary sewers will be treated. Proper sludge application into the soil takes into account agronomic loading of pollutants or nutrients. In this case, that will be the ammonia loading.