Aggregation stream of cells expressing a CRAC GFP fusion protein.
Cells move towards an aggregation centre in the lower left hand corner.
CRAC GFP accumulates at the leading edge of many cells each time a cAMP wave passes. Note how the cells surround the rear ends of the cells their are following giving rise to an U shaped profile of the leading edge. The cells move from left to right, while the cAMP waves pass from right to left. It can be seen that the cells clearly bind more GRP1-GFP at their leading edge. Mound of CRAC GFP expressing cells.