These methods genotype microsatellite data by deconvolution analysis of a marker's PCR stutter bands, thereby mathematically eliminating the stutter artifact and inferring bands that correspond to just the marker's actual allele sizes present in the sample. Available software analysis programs for automated DNA sequencers do not meet these requirements. We have developed genetic analysis software in the MatLab programming language that meets these quantitative requirements.
Analysis starts with a computer file of a gel electrophoresis image.
Program output is a text file suitable for deconvolution analysis and allele calling. MatLab runs on Macintosh, PC, and UNIX systems. Back to FAST-MAP papers .