Special equipment for precise controlling of the cooling rates is available on the market. Such equipment is very expensive and usually only needed for very sensitive cells. To achieve a similar cooling rate, the cryo vials can be placed in an insulated cube of approximately 1 litre capacity. The insulation can be paper, cotton, wool, or styrofoam. C, as no biological process takes place below this temperature.
C tend to be less stable.
C if the tanks are opened frequently. The temperature at the topof the tank rises as the nitrogen evaporates from the tank. Therefore, a temperature gradient is established from the surface of the liquid nitrogen towards the top of the tank. Protocol for thawing of cells To obtain the best possible survival of the cells, thawing of the cells must be performed as quickly as possible. If liquid nitrogen has entered the CryoTube vial, it will explode upon warming, because liquid nitrogen will expand 700 times during the warming process.