Document ID: EPA-HQ-OPP-2007-0498-0044
Agency: epa
Document Type: Supporting & Related Material
Title: 
Posted Date: 2007-10-15T04:00Z

Characteristic Underlying the Tank Measure 	Sexes Analyzed Separately 
Alternative Hypothesis Used for Comparisons to Control 	WLI	WLI	IGB	IGB

No.	Description

	E2	Atrazine	E2	Atrazine

1	Tadpole mortality	None	2

2	Failure to complete metamorphosis	None	2

3	Age at completion of metamorphosis	M and F	2	M

M, F

	4	Body weight	M and F	2

F: 0.01, 1, 100

5	Snout-to-vent length	M and F	2

F: 0.01, 1, 100

6	Gonadal image area	M and F	2	M	M:100	M

	7	Intersex (gross observation)	None	1+

8	Mixed tissue types (gross observation)	None	1+	None

None

	9	Gonadal adhesions	M and F	1+

10	Gonadal aplasia	M and F	1+

11	Gonadal segmental aplasia	M and F	1+

	M

	12	Gonadal bifurcation	M and F	1+

13	Gonadal deformity	M and F	1+

14	Gonadal displacement	M and F	1+

15	Fused gonads	M and F	1+

16	Gonadal hypertrophy	M and F	1+

17	Gonadal segmental hypertrophy	M and F	1+

F: 25

18	Gonadal hypoplasia	M and F	1+	M	M: 0.10	F

	19	Gonadal segmental hypoplasia	M and F	1+

	F	F: 0.10, 25

20	Gonadal translucence	M and F	2

	M

	21	Gonadal segmental translucence	M and F	2	(EPA F)	F: 0.10, 25, 100;
M: 100

22	Gonadal pearling	M and F	1+

23	Melanophores decreased in ovaries	F only	1+

24	Internal melanophores in testes	M only	1+

25	Frequency of males among just males and females	None	2	None

None

	26	Frequency of males	None	2	None

None

	28	Frequency of intersex	None	1+

29	Frequency of mixed sex	None	1+	None

None

	

Characteristic Underlying the Tank Measure 	Sexes Analyzed Separately 
Alternative Hypothesis Used for Comparisons to Control 	WLI	WLI	IGB	IGB

No.	TREND

Description

	E2	Atrazine	E2	Atrazine

1	Tadpole mortality	None	2

2	Failure to complete metamorphosis	None	2

3	Age at completion of metamorphosis	M and F	2	M

M, F

	4	Body weight	M and F	2

5	Snout-to-vent length	M and F	2

6	Gonadal image area	M and F	2	M

M	M: 100, 25, 1 Decreasing

7	Intersex (gross observation)	None	1+

8	Mixed tissue types (gross observation)	None	1+	None

None

	9	Gonadal adhesions	M and F	1+

10	Gonadal aplasia	M and F	1+

11	Gonadal segmental aplasia	M and F	1+

	M

	12	Gonadal bifurcation	M and F	1+

13	Gonadal deformity	M and F	1+

14	Gonadal displacement	M and F	1+

15	Fused gonads	M and F	1+

16	Gonadal hypertrophy	M and F	1+

17	Gonadal segmental hypertrophy	M and F	1+

18	Gonadal hypoplasia	M and F	1+	M

F

	19	Gonadal segmental hypoplasia	M and F	1+

	F	M: 100, 25

20	Gonadal translucence	M and F	2

	M

	21	Gonadal segmental translucence	M and F	2	(EPA F)	F: 100, 25; (EPA 1,
0.1)

M: 100

22	Gonadal pearling	M and F	1+

23	Melanophores decreased in ovaries	F only	1+

F: 100

24	Internal melanophores in testes	M only	1+

25	Frequency of males among just M & F	None	2	None

None

	26	Frequency of males	None	2	None

None

	28	Frequency of intersex	None	1+

29	Frequency of mixed sex	None	1+	None

None

	



Characteristic Underlying the Tank Measure 1 	Sexes Analyzed Separately
Alternative Hypothesis Used for Comparisons to Control	WLI	WLI	IGB	IGB

No.	Description

	E2	Atrazine	E2	Atrazine

1	Segmental hypoplasia	M and F	1+

2	Segmental aplasia	M and F	1+

3	Decreased germ cells in gonad	M and F	1+

4	Dilated testis tubules	M	1+	>0,>1

>0,>1

	5	Dividing gonocytes in testis	M	1+	>0,>1

>0,>1

	6	Internal melanophores in testis	M	1+	>0,>1

>0,>1

	7	Decreased ovarian pigmentation	F	1+

8	Decreased ovarian cavity size	F	1+	>1

	9	Increased ovarian cavity size	F	1+	>0

>0

	10	Ovarian interstitial fibrosis	F	1+

11	Mononuclear cell infiltrates	F	1+

12	Ovary stage	F	2

13	Fused kidneys	M and F	2

(EPA M >0

at 1.0)

14	Mineralization	M and F	2

15	All other renal diagnoses combined 	M and F	2



Characteristic Underlying the Tank Measure 1 	Sexes Analyzed Separately 
Alternative Hypothesis Used for Comparisons to Control 	WLI	WLI	IGB	IGB

No.	TREND

Description

	E2	Atrazine	E2	Atrazine

1	Segmental hypoplasia	M and F	1+

2	Segmental aplasia	M and F	1+

M: >0: 100, 25

M: >1: 100

3	Decreased germ cells in gonad	M and F	1+

4	Dilated testis tubules	M	1+	>0,>1

	5	Dividing gonocytes in testis	M	1+	>0,>1

	6	Internal melanophores in testis	M	1+	>0,>1

	7	Decreased ovarian pigmentation	F	1+

>0: 100

>1: 100, 25, 1

8	Decreased ovarian cavity size	F	1+	>1

	>0: 100, 25, 1

9	Increased ovarian cavity size	F	1+	>0

	10	Ovarian interstitial fibrosis	F	1+

>0: 100

11	Mononuclear cell infiltrates	F	1+

12	Ovary stage	F	2

13	Fused kidneys	M and F	2

M: >0: 100, (EPA 25)

M: >0: 100

14	Mineralization	M and F	2

M: >0: 100

15	All other renal diagnoses combined 	M and F	2

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