Document ID: EPA-HQ-OPPT-2002-0056-0040
Agency: epa
Document Type: Supporting & Related Material
Title: 
Posted Date: 2002-09-17T04:00Z

&
:
"x
,.
Pacific
Northwest
4
.,
t
i$&­*,
,s&
a:,
National
Laboratory
q&$
&@
r,
fc,
Operated
by
Battelle
for
the
.
,!
Ji.
f"
f:­
I'
!..
:
G
;!?<..
I,
y.~,`;^,
L
­*
/,..:.
,.
'
7­
6
i
*vii
?I,
U.
S.
Department
of
Energy
Chemical
Dosimetry
Group
FINAL
REPORT
Battelle
Project,
No.
41608
October
18,200l
Pharmacokinetics
of
1,1,2­
Trichloroethane
in
Rats
and
Mice
Battelle
Project
No.
41608
T.
S.
PoetI,
T.
L.
Curti,
T.
M.
Luders',
H.
Wu',
K.
G.
Studniski',
K.
K.
Weitzl,
and
R.
A.
Corleyl
`Chemical
Dosimetry
2Small
Animal
Sciences
group
Molecular
Biosciences
Department
902
Battelle
Boulevard
P.
O.
Box
999,
MSIN
P7­
59
Richland,
WA
99352
FOR
Dr.
Michael
L.
Gargas,
Representing
the
HAP
Task
Force
The
Sapphire
Group
4027
Col.
Glenn
Hwy
4*
Floor
Beavercreek,
OH
45431
Corresponding
Author:
Torka
S.
Poet,
Ph.
D.
ph:
509­
376­
7740
fax:
509­
376­
9064
e­
mail:
torka.
poet@
onl.
aov
1,1,2+
richlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
2
of
69
ABSTRACT
This
report
summarizes
the
pharmacokinetic
analyses
of
1,1,2­
trichloroethane
in
female
F344
rats
and
B6C3Fl
mice.
Studies
were
conducted
in
support
of
PBPK
modeling
efforts
by
The
Sapphire
Group.
In­
life
studies
were
conducted
at
either
WIL
Laboratories
or
at
Battelle.
Blood
samples
from
the
subchronic
inhalation
studies
performed
at
WIL
Laboratories
were
sent
to
Battelle,
Pacific
Northwest
Division
for
analysis.
In
mice,
maximal
blood
concentrations
after
4
weeks
of
inhalation
exposure
to
target
concentrations
of
100
ppm
were
approximately
2.1
ug/
ml.
In
rats,
maximal
measured
blood
concentrations
following
the
4
week
inhalation
studies
were
approximately
2.2
ug/
ml.
All
other
studies
described
in
this
report
were
conducted
at
Battelle,
Pacific
Northwest
Division.
Female
rats
and
mice
were
gavaged
with
1,1,2­
TCE
in
corn
oil
at
target
doses
of
92
or
390
mg/
kg/
day
for
5
days,
respectively,
or
in
water
at
target
doses
of
1.7
or
10
mg/
kg/
day,
respectively.
Following
exposures
to
the
higher
concentrations
in
corn
oil,
in
mice
the
lowest
blood
levels
were
observed
at
day
one
and
in
rats,
the
highest
blood
levels
were
obtained
on
day
one.
This
observation
is
consistent
with
results
from
the
inhalation
exposures
conducted
at
WIL
Laboratories,

The
off­
gassing
of
1,1,2­
TCE
was
followed
using
an
MS/
MS
real­
time
breath
analysis
system
following
inhalation
exposures
of
female
mice
to
1000,
500,
or
250
ppm
for
4
to
6
hr.
Overt
toxicity
was
observed
during
1000
ppm
exposures.
Following
exposures
all
5
exposed
animals
were
placed
in
a
2.2
L
off­
gassing
chamber,
maximal
chamber
concentrations
were
approximately
6
and
17
ppm
for
mice
exposed
to
250
or
500
ppm,

respectively.
In
addition,
partition
coefficients
were
measured
in
mouse
blood
and
rat
brain
and
spleen.
Partition
coefficients
for
1,1,2­
TCE
for
mouse
blood:
air,
rat
spleen:
air,

and
rat
brain:
air
were
71,
43,
and
56,
respectively.

This
report
was
submitted
in
fulfillment
of
Agreement
No.
41608,
by
Battele
Memorial
Institute.
This
report
covers
a
period
from
December,
2000
to
August,
2001
and
work
was
completed
as
of
&Iv,
2001.
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
3
of
69
INTRODUCTION
1,1,2­
Trichloroethane
(1,1,2­
TCE)
is
used
as
an
intermediate
in
the
production
of
vinylidene
chloride,
as
a
solvent,
in
adhesives
and
lacquers,
and
in
the
production
of
Teflon@.
In
long­
term
carcinogenicity
studies,
1,1,2­
TCE
administered
by
gavage
resulted
in
hepatic
carcinomas
and
adrenal
gland
tumors
in
both
male
and
female
l36C3Fl
mice.
No
incidences
of
neoplasms
were
detected
in
Osborn
Mendel
rats
(NTP,

1978).
Under
section
4
of
the
Toxic
Substances
Control
Act
(TSCA),
the
USEPA
issued
a
testing
consent
order
incorporating
an
enforceable
consent
agreement
with
the
HAP
Task
Force
to
perform
toxicity
testing
and
mechanistic
and
pharmacokinetic
studies
to
satisfy
EPA's
data
needs
for
1,1,2­
TCE
(Federal
Register
65(
116),
June
15,
2000,

Notices,
~~
37550­
37553).
A
PBPK
model
will
be
used
to
perform
route­
to­
route,
high­

to­
low
dose,
and
species­
to­
species
extrapolations
in
the
characterization
of
certain
potential
health
hazards
resulting
from
inhalation
exposures
based
upon
toxicity
studies
in
rats
and
mice.

Purpose
of
study
Studies
described
herein
were
designed
to
support
the
development
and
validation
of
a
physiologically
based
pharmacokinetic
(PBPK)
model
for
1,1,2­
TCE
under
development
by
The
Sapphire
Group.
The
information
in
this
report
is
restricted
to
kinetic
and
solubility
data
that
may
be
better
interpreted
following
the
PBPK
modeling
efforts.
Therefore,
this
report
is
meant
to
principally
describe
the
concentration
data
with
little
discussion
or
interpretation
of
results.
This
report
describes
the
blood
concentration
results
from
kinetic
studies
conducted
at
either
Battelle,
or
WIL
Laboratories.
Oral
gavage
kinetic
studies
in
female
B6C3Fl
mice
and
female
F344
rats
using
either
a
corn
oil
or
water
vehicle,
partition
coefficient
determinations,
and
inhalation
studies
were
conducted
by
the
Chemical
Dosimetry
Group
at
Battelle
Northwest.
A
subchronic
inhalation
study
was
conducted
by
WIL
.

Laboratories
(Ashland,
Ohio),
and
samples
collected
at
times
on
days
1,
3,
and
5
of
the
4t"
week,
The
partition
coefficient
determinations
and
the
single
inhalation
studies
were
performed
to
determine
tissue
solubility
and
to
provide
data
that
will
be
used
to
1,1,23richlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
4
of
69
estimate
metabolic
rate
constants
for
the
mouse
PBPK
model.
In
all,
5
separate
studies
were
conducted
and
are
described:

Study
1.
Demonstration
of
Periodicity
Following
Repeated
Inhalation
Exposures.

(exposures
conducted
at
WIL
Research
Laboratories)

Study
2.
Demonstration
of
Periodicity
Following
Repeated
Corn
Oil
Oral
Gavage
Administrations.

Study
3.
Demonstration
of
Periodic&
y
Following
Repeated
Aqueous
Oral
Gavage
Administrations.

Study
4.
Determination
of
Metabolic
Parameters.

Study
5.
Determination
of
Partition
Coefficients.

MATERIALS
AND
METHODS
Test
Mat&
a/
s
and
Chemicak
1,1,2­
Trichloroethane
was
obtained
from
Dow
Chemical
Co.
(Midland,
MI).
Test
material
was
99.5%
pure
as
reported
by
the
supplier.

All
other
compounds
and
solvents
were
reagent
grade
or
better.

Test
Animak
For
studies
conducted
at
Battelle
(studies
#2­
5),
adult
(8
week
old)

female
F344
rats
or
B6C3Fl
mice
were
purchased
from
Charles
River
Laboratories,

Raleigh,
NC.
The
animals
were
housed
in
suspended
plastic
cages
with
chipped
bedding
and
acclimated
to
the
laboratory
for
at
least
5
days
prior
to
dosing
with
1,1,2­
TCE.
The
rooms
in
which
the
animals
were
housed
were
on
a
12­
hr
light
cycle
(7
am
­
7
pm)
and
designed
to
maintain
adequate
temperatures,
relative
humidity,
and
airflows
for
the
species
under
study.
Deionized
water
(reverse
osmosis)
and
Purina
Certified
Rodent
Chow
#5002
(Purina
Mills,
Inc.,
St.
Louis,
MO)
were
provided
ad
hiturn.
During
the
acclimation
period,
animals
were
uniquely
marked
with
a
tail
tattoo,
weighed,
and
randomly
assigned
to
subgroups
based
upon
targeted
sacrifice
times.
Animal
weights
were
recorded
on
each
day
of
exposure.
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
5
of
69
Blood
Analysis.
Samples
of
heparinized
whole
blood
were
analyzed
for
1,1,2­
TCE
by
headspace
gas
chromatography.
Chloroform
was
used
as
an
internal
standard.
Blood
was
collected
from
control
{untreated)
female
rats
or
mice
and
standard
curves
were
generated
in
a
blood
matrix.
The
standard
curve
was
linear
up
to
58.9
pg/
ml
and
the
limit
of
detection
was
0.01
ug/
ml.
Aliquots
of
each
blood
sample
(­
O.
lg)
were
added
to
a
20ml
headspace
vial.
The
20
ml
headspace
vials
were
incubated
in
a
100°
C
oven
for
5
minutes
before
GC
analysis.

GC/
ECD
analyses
were
performed
on
a
Hewlett
Packard
6890
Series
gas
chromatograph
equipped
with
an
electron
capture
detector
(ECD).
Separations
were
achieved
with
a
J&
W
(cat.#
125­
1334)
DB­
624
fused
silica
capillary
column
(30
m
x
0.53
mmid,
3
urn
film
thickness;
J&
W,
Folsom,
CA).
Injections
were
splitless
using
a
4
mmid
Carbo­
Frit
liner.

Study
1.
Demonstration
of
Periodicity
Following
Repeated
Inhalation
Exposures.

Study
Design.
Under
a
separate
contract,
WI1
Laboratories
conducted
a
subchronic
inhalation
study
with
1,1,2­
TCE
in
rats
and
mice,
which
included
subgroups
of
animals
for
pharmacokinetic
determinations.
Groups
of
3
female
F344
rats
or
3
female
B6C3Fl
mice
per
sample
time
(36
animals/
species)
were
sacrificed
and
blood
collected
by
WIL
laboratories
at
target
times
of
4,
6,
6.25
and
8
hr
after
the
start
of
the
6­
hr
exposures
to
a
target
concentration
of
100
ppm
TCE
on
exposures
days
1,
3
and
5
of
the
4th
week
of
the
study.
On
day
1,
target
exposure
time
and
blood
sample
collection
were
all
delayed
1
hr
such
that
target
times
were
5,
7,
7.25,
and
9
hr.
Aliquots
of
100
ul
of
blood
were
placed
in
headspace
vials
which
were
tightly
sealed
and
the
weight
of
the
blood
recorded.
Analyses
were
limited
to
the
determination
of
1,1,2­
TCE
in
blood
samples
under
this
statement
of
work.
However,
other
tissue
samples
(brain,
lung,

liver,
spleen
and
kidney)
were
collected
and
stored
frozen
for
potential
future
analyses.

Travel
and
storage
quality
control
spikes
containing
known
amounts
of
1,1,2­
TCE
in
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
6
of
69
each
sample
matrix
were
prepared
by
WIL
Laboratories
to
be
included
in
the
analysis,

Individual
animal
blood
1,1,2­
TCE
exposure
conditions
and
concentration
data
are
given
in
Appendix
A.

Study
2
and
3.
Demonstration
of
Periodicity
Following
Repeated
Oral
Gavage
Administrations
in
either
Corn
Oil
or
Water.

Study
Design.
A
total
of
4
gavage
studies
were
conducted.
Group
1
mice
(animal
#s
MOOl­
M038)
were
administered
1,1,2­
TCE
for
1
to
5
days
by
gavage
at
a
target
dose
of
390
mg/
kg/
day
in
corn
oil.
Group
2
mice
(animal
#s
MlOl­
M138)
were
administered
1,1,2­
TCE
for
1
to
5
days
by
gavage
at
a
target
dose
of
9.5
mg/
kg/
day
in
water.
Group
1
rats
(animal
#s
ROOl­
R038)
were
administered
1,1,2­
TCE
for
1
to
5
days
by
gavage
at
a
target
dose
of
92
mg/
kg/
day
in
corn
oil.
Group
2
rats
(animal
#s
RlOl­
R138)
were
administered
1,1,2­
TCE
for
1
to
5
days
by
gavage
at
target
dose
of
1.7
mg/
kg/
day
in
water.
Two
extra
animals/
study
were
administered
1,1,2­
TCE
as
potential
replacements
in
the
event
that
any
problems
were
encountered
during
dosing
or
sacrifice.

Dose
Solutions
and
Administration.
Corn
oil
solutions
of
1,1,2­
TCE
were
prepared
late
on
the
Friday
for
administration
on
the
following
Monday,
and
stored
at
­80°
C.
Aliquots
of
dosing
solution
were
stored
for
GC
concentration
check
analysis,
For
the
390
mg/
kg/
day
target
dose
(mice),
a
target
concentration
of
39
mg/
ml
was
prepared.
For
the
92
mg/
ml/
day
dose
(rats),
a
target
concentration
of
46
mg/
ml
was
prepared,
These
target
doses
were
chosen
to
match
the
highest
dose
administered
in
the
NTP
chronic
bioassay
(NTP,
1978).

Due
to
the
low
solubility
of
1,1,2­
TCE,
water
target
concentrations
were
less
than
1
mg/
ml.
The
stated
water
solubility
for
1,1,2­
TCE
is
0.45%,
a
sotubility
check
confirmed
this
as
the
maximum,
but
in
repeated
checks
of
homogeneity,
it
was
determined
that
1
mg/
ml
was
the
maximum
concentration
of
1,1,2­
TCE
that
was
consistently
homogenous.
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
7
of
69
Samples
of
each
dose
solution
were
taken
for
analysis
on
the
day
of
dosing
to
confirm
targeted
concentrations.
Each
mouse
was
weighed
and
administered
dose
solutions
by
gavage
at
a
rate
of
­10
ml/
kg
body
weight
to
achieve
the
targeted
dose
levels.
Each
rat
was
weighed
and
administered
dose
solutions
by
gavage
at
a
rate
of
­2
ml/
kg
body
weight
to
achieve
the
target
dose
levels.
The
dosing
syringe
was
weighed
before
and
after
dosing
to
determine
the
actual
volume
delivered
to
each
animal.

Specimen
Collection.
Scheduled
sacrifice
times
were
0.5,
1,
2,
and
8
hr
for
the
corn
oil
gavage
studies
and
0.1,
0.25,
0.5,
and
1
hr
for
the
water
gavage
studies.
At
each
scheduled
sacrifice
time,
animals
were
anesthetized
in
an
80%
CO2
atmosphere
and
blood
samples
were
collected
by
closed­
chest
cardiac
puncture.
To
avoid
volatilization
loss
of
1,1,2­
TCE,
blood
samples
were
quickly
aliquoted
into
duplicate
samples
of
100
ul
each
into
headspace
vials
which
were
immediately
sealed.
The
headspace
vials
were
weighed
and
immediately
frozen
on
dry
ice.
In
some
cases
for
mice,
only
a
single
100
pl
sample
was
available.
Any
remaining
blood
was
placed
into
chilled
heparinized
VacutainersB
in
the
event
that
additional
sample
might
be
needed.
The
time
of
death
was
recorded
at
blood
draw
and
all
animals
were
rapidly
dissected
to
remove,
trim
extraneous
tissues,
and
weigh
the
kidneys,
spleen,
liver,
lung,
and
brain.
The
time
at
which
the
final
tissue
(brain)
was
removed
was
also
recorded.
The
tissues
were
flash­

frozen
and
stored
along
with
the
blood
samples
at
­8O'C.

Study
4.
Determination
of
Metabolic
Parameters.

Study
Resign.
To
provide
data
to
be
used
in
the
PBPK
model
to
determine
metabolic
rate
constants,
five
female
B6C3Fl
mice
were
exposed
by
whole­
body
inhalation
for
4
to
6
hr
to
three
separate
exposure
concentrations
(1000,
500,
and
250
ppm).

Immediately
following
exposure,
all
5
mice
were
placed
in
an
off­
gassing
chamber
and
1,1,2­
TCE
measured
by
direct
sampling
of
the
chamber
air
using
an
ion
trap
mass
spectrometer
(MS/
MS)

Vapor
Generation
System.
High
purity
breathing
air
was
regulated
through
a
Kin­
Tek
Laboratories
670­
C
Precision
Gas
Standards
Generator
at
a
flow
rate
of
approximately
2.0
L/
min.
The
standard
generator
was
loaded
with
three
15
ml
diffusion
tubes
filled
at
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
8
of
69
half
capacity
with
1,1,2­
TCE.
The
exposure
atmosphere
was
delivered
through
a
stainless
steel
circular
tube
located
at
the
bottom
of
the
glass
exposure
chamber.

Chamber
pressurization
was
avoided
by
using
an
unobstructed
%"
exhaust
port
located
on
the
top
chamber
lid.
Target
concentrations
were
obtained
by
varying
the
diffusion
cell
temperature.

Whole
Body
Exposure
Chamber.
A
dynamic
flow­
through
inhalation
chamber
was
designed
to
allow
for
rapid
changeover
between
the
closed,
recirculating
chamber
and
a
dynamic,
fiow­
through
operation,
Each
mouse
was
weighed
and
5
animals
were
exposed
to
target
concentrations
of
either
250,
500
or
1000
ppm
1,1,2­
TCE
for
up
to
6
hr
in
an
8.59­
L
glass
and
stainless
steel
closed
inhalation
chamber.
After
the
completion
of
each
exposure,
the
animals
were
placed
in
off­
gassing
system
described
below.

Chamber
Monitoring.
Exposure
concentrations
of
1,1,2­
TCE
were
determined
by
gas
chromatographic
analysis
of
atmosphere
from
the
exposure
chamber
using
a
Hewlett
Packard
5890
Series
II
capillary
gas
chromatograph
with
flame
ionization
detection
(GC/
FID;
Hewlett
Packard,
Palo
Alto,
CA).
A
30
m
x
0.32
mm
ID
DB­
624
with
3
pm
film
thickness
capillary
column
was
used
to
separate
each
test
chemical
from
the
air
(J&
W
Scientific,
Folsom,
CA).
The
chemical
concentration
during
each
exposure
was
monitored
approximately
every
8
min
via
automatic
injections
of
­1
mL
of
chamber
air
through
a
Valco
gas
sampling
valve.
The
GC/
FID
was
calibrated
with
a
standard
curve
consisting
of
6
standards
prepared
in
3­
L
TedlarD
bags
(SKC,
Fullerton,
CA)
prior
to
each
exposure.

Off­
Gassing,
Immediately
following
the
inhalation
exposures,
all
5
mice
were
placed
in
a
2.2
L
off­
gassing
chamber.
Breathing
air
was
continually
supplied
to
the
mice
through
the
lid
of
the
off­
gassing
chamber
at
a
measured
rate
(179­
183
ml/
min).
Airflow
rates
were
measured
using
flow
meters
from
Sierra
Instruments
(Carmel
Valley,
CA)
and
were
calibrated
prior
to
use.
A
Teledyne
Discovery
II
MS/
MS
equipped
with
an
atmospheric
sampling
glow
discharge
ionization
(ASGDI)
source
sampled
from
the
off­

gassing
chamber
(representing
exhalation
from
the
animal)
approximately
every
5­
set
for
up
to
9
hr
following
the
inhalation
exposure.
The
ASGDI
source
derived
reagent
ions
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
9
of
69
directly
from
the
volatile
chemicals
in
the
sampled
air.
An
electric
potential
was
established
by
applying
400
V
between
two
plates.
Ions
were
then
focused
onto
the
MS/
MS
trap.
Helium
was
used
as
a
buffer
and
collision
gas.
The
intensity
data
from
the
MS/
MS
was
converted
to
concentration
(ppb)
through
the
use
of
external
standards
prepared
in
Tedlar@
bags.
A
standard
curve
was
generated
each
day
of
experimentation
1,1,2­
TCE
was
quantified
by
selective
ion
monitoring
of
the
m­
l
(m/
z
83)
and
m+
l
(m/
z
85)
ions
of
the
daughter
produced.

Study
5.
Determination
of
Partition
Coefficients,

Liquid:
air
and
tissue:
air
partition
coefficients
were
determined
for
mouse
blood
and
rat
brain
and
spleen
using
the
vial
equilibration
technique
of
Gargas
et
a/.
(1989).
Tissues
were
obtained
from
5
individual
rats
and
6
individml
mice,
and
blood
was
pooled
from
4
additional
mice.
Tissues
were
collected
from
the
rats,
rinsed
in
phosphate
buffered
saline,
and
homogenized
in
3
x
v/
w
PBS.
Blood
was
collected
in
heparinized
syringes.

1.0
ml
of
tissue
homogenate
was
incubated
with
shaking
for
1
hr
and
the
headspace
sampled
for
1,1,2­
TCE.
Due
to
the
low
volume
of
mouse
blood
obtained,
0.5
ml
of
whole
blood
was
incubated
for
1
hr.
Headspace
1,1,2­
TCE
concentrations
were
compared
to
vials
containing
1,1,2­
TCE
vapor
only
for
blood
or
vials
containing
0.75
ml
saline
for
the
tissues.
A
pilot
study
with
rat
blood
was
used
to
verify
that
1
hr
incubations
with
0.5
ml
of
blood
were­
sufficient
to
reach
steady
state,

RESULTS
Study
1.
Demonstration
of
Periodicity
Following
Repeated
Inhalation
Exposures.

Samples
of
blood
(~
100
ul)
from
inhalation
studies
in
rats
and
mice
were
supplied
by
.

WIL
Laboratories
in
headspace
vials
with
recorded
gravimetric
weights,
In
addition,
WIL
Laboratories
provided
control
blood
for
the
generation
of
standard
curves
and
storage
1,1,2+
richlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
10
of
69
spikes
with
known
quantities
of
1,1,2­
TCE.
The
1,1,2­
TCE
concentrations
in
the
storage
spikes
were
quantified
using
GC
headspace
analysis.
To
calculate
the
expected
concentrations,
the
supplied
gravimetric
data
was
used
for
volumes
of
blood
and
spiking
solution
added
and
the
stock
solution
concentration
as
analyzed
using
GC
headspace
analysis.
Recoveries
of
1,1,2­
TCE
in
storage
spikes
averaged
98.2
and
99.9%
for
mouse
and
rat
blood,
respectively,
verifying
that
the
sample
collection,

shipping,
and
storage
methods
for
this
study
were
adequate
(Table
1).

Blood
concentrations
in
female
B6C3Fl
mice
on
days
1,
3,
and
5
of
week
4
were
analyzed
using
GC
headspace
methods.
The
blood
concentrations
during
and
after
the
inhalation
exposures
show
the
typical
uptake
and
elimination
profile
for
this
type
of
exposure
(Fig
1).
For
mice,
the
blood
concentration
peaked
at
the
6
hr
target
timepoint
on
day
5
at
2.2
+
0.37
ug/
g
blood.
The
peak
concentration
achieved
in
rat
blood
on
day
1
at
the
7
hr
target
timepoint
was
2.3
t­
0.35
ug/
g
blood.

Study
2
and
3.
Demonstration
of
Periodic&
y
Following
Repeated
Oral
Gavage
Administrations
in
either
Corn
Oil
or
Water,

1,1,2­
TCE
was
administered
to
female
B6C3Fl
mice
or
rats
in
either
a
corn
oil
vehicle
or
water
vehicle.
Due
to
the
limited
solubility
of
1,1,2­
TCE
in
water,
the
target
doses
in
water
were
IO
mg/
kg
for
mice
and
1.7
mg/
kg
in
rats.
Animals
were
sacrificed
at
times
following
administration
on
days
1,
3,
or
5
of
the
5
day
dosing
schedule
and
blood
and
tissues
collected.
Blood
was
analyzed
for
1,1,2­
TCE
concentrations.
Individual
animal
body
weight,
sacrifice
time,
dose
administered,
and
blood
1,1,2­
TCE
concentration
data
are
given
in
Appendix
B.

Dose
Sohtions
and
Adminstration.
Actual
concentrations
of
1,1,2­
TCE
in
the
dose
solutions
were
within
10%
of
target
by
gravimetric
and
GCjECD
analyses
and
were
considered
homogeneous
(Tables
2
and
3).
The
final
GC/
ECD
analyzed
concentrations
were
used
in
determinations
of
actual
doses
of
1,1,2­
TCE
in
subsequent
analyses,

The
dosing
syringes
were
weighed
immediately
prior
to
and
immediately
after
dosing
and
the
actual
volume
dosed
determined
gravimetrically.
The
density
of
corn
oil
(0.92
1,1,2­
trichiorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
11
of
69
g/
ml)
was
included
in
the
dosing
concentration
calculations.
The
average
actual
time
of
sacrifice,
body
weights,
and
1,1,2­
TCE
concentrations
administered
on
the
day
of
sacrifice
are
given
in
Tables
4­
7
for
animals
administered
1,1,2­
TCE
by
gavage
either
in
corn
oil
or
water.
Body
weights
were
determined
immediately
before
dosing
on
each
day.
The.
daily
body
weights
of
the
animals
from
the
group
administered
1,1,2­
ICE
for
all
5
days
are
shown
in
Figure
2.

Blood
Concentrations
following
Corn
Oil
Gavage.
In
mice,
the
peak
blood
concentration
after
corn
oil
gavage
was
observed
at
day
5
at
the
first
timepoint,
0.5
hr
following
dosing.
The
blood
concentrations
from
day
1
dropped
more
rapidly
than
on
days
3
and
5
of
dosing
(Fig.
3).
In
rats,
peak
blood
concentrations
were
observed
at
the
first
timepoint
on
day
1
of
dosing
(Fig.
4).

Blood
Concentrations
fallowing
Water
Gavage.
In
both
rats
and
mice,
peak
1,1,2­
TCE
blood
concentrations
were
observed
at
the
first
timepoint
(6
min)
on
the
first
day
of
dosing
following
administration
of
1,1,2­
TCE
in
a
water
vehicle
(Fig.
5
and
6).

Terminal
Body
and
Tissue
Weights.
Lungs,
liver,
kidneys,
spleen
and
brain
were
collected
from
each
animal
immediately
after
blood
collection
upon
sacrifice.
Tissue
weights
are
given
in
Appendix
C.
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No,
41608
Page
12
of
69
Study
4.
Determination
of
Metabolic
Parameters.

Prefiminarygavage
study.
A
preliminary
study
was
conducted
to
determine
the
utility
of
the
real
time
MS/
MS
breath
analysis
system
to
detect
1,1,2­
TCE
in
mice
following
a
gavage
exposure.
For
the
preliminary
study,
5
mice
weighing
from
22­
24
g
were
gavaged
with
26.6
mg/
kg
1,1,2­
TCE
in
a
corn
oil
vehicle
and
placed
in
the
2.2­
L
off­

gassing
chamber.
Dosing
and
animal
weight
information
is
given
in
Table
8.
It
took
an
average
of
10
minutes
following
dosing
to
begin
off­
gassing
analysis.
Chamber
air
was
monitored
for
3.5
hr
post
dosing,
peak
exhaled
l,
l,
2­
TCE
concentrations
were
achieved
by
30
min
and
were
no
longer
detectable
by
1.5
hr
post­
dosing
(Fig
7).

Inhalatiun
Exposures.
Female
B6C3Fl
mice
were
exposed
to
1,1,2­
TCE
by
inhalation
at
concentrations
of
250,
500,
or
1000
ppm
for
4
to
6
hr
(Fig.
8).
Immediately
after
the
exposure,
all
5
animals
were
placed
in
an
off­
gassing
chamber
and
chamber
1,1,2­
TCE
concentrations
monitored
for
up
to
9
hr.
The
exposure
to
1000
ppm
resulted
in
extreme
lethargy.
About
4
hr
post
exposure
1
animal
died,
at
6
hr
post­
exposure
all
remaining
animals
were
still
lethargic
or
unconscious.
The
exhaled
breath
profile
from
these
animals
was
not
typical
(Fig
9).
Body
weights,
exposure
and
off­
gassing
conditions,
and
comments
are
given
in
Appendix
D.

Due
to
indications
of
developing
solvent­
type
anesthesia,
the
exposure
to
500
ppm
was
stopped
after
4
hr.
In
the
subchronic
inhalation
studies
conducted
by
Wil
Laboratories,

equilibrium
was
reached
by
4
hr.
Animals
showed
no
overt
effects
following
250
ppm
exposures
for
6
hr.
Exhaled
breath
profiles
are
shown
in
Fig
9.

Study
5.
Determination
of
Partition
Coefficients.

A
pilot
study
using
frozen
blood
from
male
F344
rats
was
performed
to
assess
the
length
of
incubation
time
and
validate
the
use
of
less
than
1
ml
of
blood.
Results
and
volumes
are
given
in
Appendix
E.
Based
on
the
results
from
this
study,
incubations
containing
0.5
ml
of
female
B6C3Fl
mouse
blood
or
1
ml
of
female
F344
rat
tissue
were
1,1,2+
richlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
13
of
69
carried
out
for
1
hr.
Tissue:
air
partition
coefficients
are
shown
in
Table
9.
Individual
incubation
data
are
given
in
Appendix
E.
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
14
of
69
DISCUSSION
Target
corn
oil
gavage
doses
of
390
mg/
kg/
day
in
mice
and
92
mg/
kg/
day
in
rats
were
chosen
based
on
the
high
dose
from
the
NTP
chronic
bioassay
(NTP,
1978).
The
doses
for
the
gavage
exposures
in
water
were
based
on
limit
of
solubility.
Blood
elimination
profiles
indicate
that
periodicity
was
reached
over
5
consecutive
days
of
dosing.
This
is
consistent
with
blood
analysis
from
rats
and
mice
exposed
via
inhalation
for
4
weeks.

The
4­
6
hr
inhalation
exposures
indicated
a
more
pronounced
effect
of
1,1,2­
TCE
in
mice
than
expected.
Exposure
to
the
highest
concentration
(1000
ppm)
resulted
in
severe
sedation
and
mortality
in
one
animal,
thus
the
off­
gassing
data
from
this
high
concentration
is
not
viable
for
use
in
a
pharmacokinetic
study.
No
anesthesia
or
adverse
affects
were
observed
during
or
following
exposures
to
250
ppm.

Based
on
a
comparison
of
partition
coefficients,
1,1,2­
TCE
may
be
slightly
more
soluble
in
blood
from
female
B6C3Fl
mice
than
from
male
F344
rats.
Partition
coefficients
in
female
F344
rat
brain
and
spleen
were
56
and
43,
respectively.
l&&
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
15
of
69
Reference
List
Gargas,
M.
L.,
Burgess,
R.
J.,
Voisard,
D.
E.,
Cason,
G.
H.
and
Andersen,
M.
E.
(1989).
Partition
coefficients
of
low­
molecular
weight
volatile
chemicsl
in
various
liquids
and
tissues.
ToxicoL
Apple
Pbarmacol.
98,
87­
99.

Gargas,
M.
L.
(1990).
An
exhaled
breath
chamber
system
for
assessing
rates
of
metabolism
and
rates
of
gastrointestinal
absorption
with
volatile
compounds.
Journal
of
the
American
College
of
Toxicology
9,
447­
453.

NTP.
1,1,2­
Trichloroethane:
bioassay
for
possible
carcinogenicity.
074.
1978,
National
Toxicology
Program.
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
16
of
69
Mouse
1
,I
,2­
TCE
Blood
Cont.
­
Inhalation
Exposures
m
Day
1
rDay3
*Day5
0.01
1
i
/
I
0
1
2
3
4
5
6
7
8
9
Time
(hr)

Rat
1
,1,2­
TCE
Blood
Cont.
­
Inhalation
Exposures
0.01
I
,
,

0
1
2
3
4
5
6
7
a
9
Time
(hr)

Figure
1.
Blood
1,1,2­
TCE
Concentrations
in
female
B6C3Fl
Mice
(top)
and
F344
Rats
(bottom)
following
inhalation
exposures.
Female
rats
and
mice
were
exposed
to
target
concentrations
of
100
ppm
1,1,2­
TCE
for
6­
hr
/day
for
4
weeks.
Blood
was
collected
on
exposure
days
1,
3
and
5
of
the
4'
week
of
the
study.
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
17
of
69
21
­
A)
Mouse
/

.19
I
:
6
i
J
5
181
2
j
17
j
16
I
1
­+­
9.5
mg/
kg
1,
l
,P­
TCE
in
Water
I
­R­
390
mglkg
1
,I
,2­
TCE
in
Corn
Oil
15
1
2
3
4
5
Day
of
Treatment
+
1.5
mglkg
1
,1,2
TCE
in
Water
­B­
92
mg/
kg
1
,I
,2­
TCE
in
Corn
Oil
125
!
1
1
2
3
4
5
Day
of
Treatment
Figure
2.
Rat
and
mouse
body
weights
over
the
five
days
of
treatment
with
1,1,2­
ICE
in
either
corn
oil
or
water.
Body
weights
were
measured
for
each
animal
immediately
.
prior
to
dosing
on
each
day.
These
data
are
from
the
animals
treated
for
all
5
days
only
(n=
l2).
1,1,2+
richlorethane:
PK
in
rats
and
mice
Project
No.
41608
10
i;
j
h
=

=!
s
1
­8
­0
m
0.1
1
,I
,2­
TCE
in
Blood:
Mouse
390
mglkg
in
Corn
Oil
i
f
f:
i
I
Page
18
of
69
r'

1.0
2.0
3.0
4.0
Time
(hr)
5.0
6.0
7.0
8.0
Figure
3.
Blood
1,1,2­
TCE
concentrations
in
female
66C3Fl
mice
following
corn
oil
gavage
with
a
target
of
390
mg/
kg/
day
for
5
days.
Mice
were
sacrificed
and
blood
taken
at
time
points
on
days
1,
3,
and
5.
1,1,2+
richlorethane:
PK
in
rats
and
mice
Project
No.
41608
1
,I
,2­
TCE
in
Blood:
Rat
92
mglkg
in
Corn
Oil
1
j
A
Day
3
l*
Day5
i
Page
19
of
69
I
0.01
!

0.0
2.0
4.0
6.0
8.0
Time
(hr)

Figure
4.
Blood
1,1,2­
TCE
concentrations
in
female
F344
rats
following
corn
oil
gavage
with
a
target
of
92
mg/
kg/
day
for
5
days.
Rats
were
sacrificed
and
blood
taken
at
time
points
on
days
1,
3,
and
5.
1,1,2+
richlorethane:
PK
in
rats
and
mice
Project
No.
41608
I
,I
,2­
TCE
in
Blood:
Mouse
9
mg/
kg
in
Water
n
Day
1
rDay3
*_
Dav
Page
20
of
69
0.0
0.2
0.4
0.6
0.8
1.0
Time
(hr)

Figure
5.
Blood
1,1,2­
TCE
concentrations
in
female
B6C3Fl
mice
following
water
gavage
with
a
target
of
10
mg/
kg/
day
for
5
days.
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
1
,I
,2­
TCE
in
Blood:
Rat
1.5
mg/
kg
in
Water
t
$
/
iDDay
j
I&
Day3
/*
Day5
)
Page
21
of
69
f
t
0.001
i
/_
0.0
0.2
0.4
0.6
0.8
1
.o
Time
(hr)

Figure
6.
Blood
1,1,2­
JCE
concentrations
in
female
F344
rats
following
water
gavage
with
a
target
of
1.7
mg/
kg/
day
for
5
days.
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
22
of
69
8o01
700
­
l
*
l
*
l
a^
600
­
l
*
l
4+
l
l
2
+*
**

­
500
­
l
*
l
+*+

E
l
l
l
s
400
­

k
+*

f
300
­
+
l
*

03
6
l
p+%
+
**

200
­
l
*
*
l
*
l
*,
l
100
­
l
0
0
0.25
0.5
0.75
1
1.25
1.5
Time
(hr)

Figure
7.
Chamber
concentrations
of
1,1,2­
TCE
following
corn
oil
gavage
WITH
26.6
mg/
kg
in
5
female
B6C3Fl
mice.
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
23
of
69
ChamberConcentration:
1,1,2­
Trichloroethane
248.3
+b
a.
54
ppm
­L~­~~­­;
r­
rt­
UrlUA~~
AAbA~
A~~
A~~~
A~~~~~.

6
100
:

01
0:
OO
0:
30
I:
00
1:
30
2:
00
2:
30
3:
00
3:
30
4:
00
4:
30
300
5:
30
6:
00
Elapsed
Time
(hr)

Figure
8,
Mice
were
exposed
to
1,1,2­
TCE
by
inhalation
exposure
for
4­
6
hr
at
target
concentrations
of
1000,
500,
and
250
ppm.
Exposure
chamber
concentrations
were
measured
approximately
every
8
minutes
over
the
time
of
exposure.
The
average
exposure
concentration
rt
SD.
is
given
above
the
data
from
each
exposure
concentration.
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
24
of
69
(
L
250
ppm
A
A­
*
,`,

*
A
4
,
I
I
I
0.0
1
.o
2.0
3.0
4.0
5.0
Time
(hr)

Figure
9.
Off­
gassing
of
1,1,2­
TCE
following
4­
6
hr
inhalation
exposures
(points
averaged
every
one
minute
interval).
Five
mice
were
exposed
to
target
concentrations
of
1000,
500,
or
250
ppm
of
1,1,2­
TCE
via
inhalation
and
off­
gassing
of
1,1,2­
TCE
measured
using
a
real­
time
MS/
MS
system.
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No,
41608
Page
25
of
69
Table
1.
Inhalation
Study
Storage
Spikes
Average
GC/
FID
%
Gravimetric
Cont.
of
Target
@g/
ml)
_+
SD
Mouse
Blood
Samples
173.6
98.2
f
8.5
Rat
Blood
Samples
135.9
99.9
f
11.8
Storage
spikes
were
provided
by
WIL
Laboratories.
The
gravimetric
concentration
was
calculated
using
weights
of
each
component
(blood
and
spike
solution)
supplied
by
WIL
Laboratories
and
the
concentration
of
the
spike
solution.
The
spike
solutions
were
also
supplied
by
WIL
Laboratories
and
analyzed
using
GC
headspace
analysis
to
determine
the
accurate
concentration.
Four
storage
spikes/
species/
day
(total
of
12
for
mice
and
12
for
rats)
were
provided.
1,1,2+
richlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
26
of
69
Table
2.
Oral
Gavage
in
Corn
Oil:
1,1,2­
TCE
Dose
Solutions.
Study
Target
Cont.
&a
vimetric
GC/
FID
Cont.
GC/
FID
96
(mg/
mg
Cont.
(mg/
ml)
(mg/
mf)
of
Target
Mouse:
390
mg/
kg
39
39.0
35.0
89.7
target
dose
Rat:
92
r­
w/
kg
target
dose
46
46.2
46.5
101.1
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
27
of
69
Table
3.
Oral
Gavage
in
Water:
1,1,2­
TCE
Dose
Solutions.
Study
Target
Cont.
Gravimetric
GC/
fID
Cont.
GC/
FID
%
(mg/
mo
Cont.
(mg/
m~
(mq/
ml)
of
Target
Mouse:
10
mg/
kg
0.9
0.895
0.891
99.0
target
dose
Rat:
1.7
mg/
kg
0.9
0.882
0.854
94.9
target
dose
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
28
of
69
Table
4,
Oral
Gavage
in
Corn
Oil:
Body
Weights,
Sacrifice
Times
and
Amounts
of
1,1,2­
TCE.
Mouse:
390
mg/
kg
target
dose
Scheduled
Sacrifice
77me
Actual
Sacrifice
Body
Wt.
at
Actual
Dose
Actual
Dose
(hr:
min)
Time
(bf:
min)
Dosing
(kqy
(mdkd
%
of
Target
0:
30
1:
oo
2:
oo
8:
00
0:
30
1:
oo
2:
oo
8:
00
0:
30
1:
oo
2:
oo
8:
00
Day1
0:
31
I
0:
Ol
0:
58
rt
0:
Ol
1:
57
­+
0:
03
8:
00
k
0:
02
Day3
0:
32
III
0:
02
0:
59
IL
0:
Ol
2:
Ol
If:
0:
Ol
7:
56
+
0:
Ol
Day5
0:
31
i
0:
Ol
0:
58
f
0:
OO
2:
Ol
i
0:
oo
8:
01
I
0:
Ol
0.019
zk
0.001
0.020
rt
0.001
0.020
i­
0.001
0.020
F
0.001
354.3
+
12.5
90.8
352.2
c
8.5
90.3
350.1
i:
7.1
89.7
351.7
k
6.4
90.2
0.020
zk
0.001
0.019
­i:
0.001
0.020
+­
0.001
0.019
rt
0.000
354.2
k
10.9
391.1
i
42.6
360.2
1
32.0
371.7
zk
8.7
90.1
100.3
92.4
95.3
0.019
z!
z
0.001
365.3
z!
z
16.2
93.7
0.019
i
0.001
399.2
+
31.3
102.3
0.019
z!
z
0.001
378.3
+
48.2
97.0
0.018
i
0.001
358.1
f
18.1
91.8
Study
Averages
I
0,019
+
0.001
365.5
+
25.8
93.7
`Animals
were
dosed
on
a
5
day
schedule,
Body
Weight
and
Actual
Dose
are
for
the
day
of
sacrifice.
The
dose
administered
was
calculated
from
the
pre­
and
post­
dosing
syringe
weights
and
the
GC
analysis
of
the
dosing
solution.
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
29of69
Table
5.
Oral
Gavage
Study:
Body
Weights,
Sacrifice
Times
and
Amounts
of
1,1,2­
TCE.

Rat:
92
mg/
kg
ta
Scheduled
Sacrifice
Time
(br:
min)

0:
30
l:
oo
2:
oo
8:
00
0:
30
l:
oo
2:
oo
8:
00
r
T
0:
30
l:
oo
2:
oo
8:
00
let
dose
Actual
SacrRe
Body
Wt.
at
Actual
Dose
Actual
Dose
%
Time
(hr:
min)
Dosing
(kgy
h­
W&?
1
'
of
Target
Day
1
0:
33
5
0:
03
0.145
i:
0.004
97.5
?c
0.9
106.0
0:
57
I
0:
06
0.144
k
0.003
95.7
+
1.6
104.0
2:
Ol
f
0:
Ol
0.145
k
0.005
96.1
t
2.6
104.5
7:
59
rt
0:
oo
0.149
i
0.003
97.0
k
3.2
105.3
Day
3
0:
30
+_
0:
02
0.148
I!
C
0.005
96.3
+­
5.0
104.6
l:
oo
k
0:
Ol
0.148
+
0.006
92.8
+
4.1
100.9
2:
Ol
i­
0:
Ol
0.147
T!
z
0.003
93.4
Lk
2.2
101.5
8:
Ol
+
0:
Ol
0.147
i
0.001
95.1
1:
5.4
103.4
Day
5
0:
30
ri:
0:
Ol
0.149
s_
0.004
93.0
i­
1.8
101.1
1:
03
i­
0:
Ol
0.147
I
0.008
93.0
c_
4.3
101.1
2:
oo
IL
0:
02
0.150
k
0.006
91.3
k
2.6
99.2
8:
02
rt
0:
Ol
0.150
i
0.006
89.7
F
4.6
97.5
Study
Aberages
I
0.147
IO.
0043
94.2
zk
3.7
102.4
`Animals
were
dosed
on
a
5
day
schedule,
Body
Weight
and
Actual
Dose
are
for
the
day
of
sacrifice.
Zrhe
dose
administered
was
calculated
using
the
pre­
and
post­
dosing
syringe
weights
and
the
GC
analysis
of
the
dosing
solution.
1,1,2­
trichforethane:
PK
in
rats
and
mice
Project
No,
41608
Page3Oof69
Table
6.
Oral
Gavage
in
Water:
Body
Weights,
Sacrifice
Times
and
Amounts
of
1,1,2­
ICE,

Mouse:
10.0
r
Scheduled
Sacrifice
77me
(hr:
min)

0:
06
0:
15
0:
30
1:
oo
O:
O6
0:
15
0:
30
l:
oo
0:
06
0:
15
0:
30
1:
oo
Stud
Day
1
0:
06:
05
k
0:
00:
09
0.019
s
0.001
9.9
­;
0.8
99.0
0:
14:
20
ic
0:
00:
17
0.019
k
0.001
10.0
I
0.9
100.0
0:
30:
10
i
0:
00:
23
0.019
r
0.001
9.5
rt
0.7
95.0
0:
59:
40
t
0:
00:
31
0.019
F
0,001
9.6
+_
0.3
96.0
Day
3
0:
07:
00
5
0:
01:
09
0.019
k
0.000
9.6
k
0.3
96.0
0:
14:
40
z!
T
0:
00:
31
0.019
F
0.001
10.6
A
2.2
106.0
0:
30:
55
+
0:
00:
46
0.019
I
0.000
10.7
zk
2.0
107.0
0:
59:
45
+_
0:
00:
15
0.019
Ik
0.000
9.5
t_
1.5
95.0
Day5
0:
06:
30
+
0:
00:
30
0.020
i
0.001
9.61
0.2
96.0
0:
15:
45
z!
z
0:
00:
15
0.020
I
0.000
9.5
k
041
95.0
0:
30:
10
+
0:
00:
23
0.019
+
0.001
9.3
­t
0.8
93.0
0:
59:
40
z!
z
0:
00:
31
0.018
?I
0.001
9.3
k
0.6
93.0
Averages
I
0.019
1.
0.001
9.7
rt
1.1
97.0
'Animals
were
dosed
on
a
5
day
schedule,
Body
Weight
and
Actual
Dose
are
for
the
day
g/
kg
target
dose
Actual
Sacritce
7ime
(hr:
min:
sec)
Body
Wt.
at
Actual
Dose
Actual
Dose
Dosing
(kg)
'
bWW
'
%
of
Target
of
sacrifice.
r
The
dose
administered
was
calculated
from
the
pre­
and
post­
dosing
syringe
weights
and
the
GC
analysis
of
the
dosing
solution.
1,1,2­
trichlorethane:
PKin
rats
and
mice
Project
No.
41608
Page31of69
Table
7.
Oral
Gavage
In
Water:
Body
Weights,
Sacrifice
Times
and
Amounts
of
1,1,2­
TCE.

Rat:
1.7
mg/
kg
target
dose
Scheduled
Actual
Sacrifice
Body
Wt.
at
Actual
Dose
Actual
Dose
Sacrifice
Time
Time
(hcmin)
Dosing
(kgy
hW~
sU
2
%
of
Target'
(hr:
min)
Day
1
0:
06
0:
06:
10
i
0:
00:
09
0.148
it
0,001
1.7
in
0.01
100.0
0:
15
0:
14:
50
+
0:
00:
31
0.147
*
0.002
1.7
i
0.09
100.0
0:
30
0:
30:
55
+
0:
00:
38
0.149
~fr
0.002
1.7
Ii
0.04
100.0
l:
oo
0:
59:
50
ic
0:
00:
31
0.148
rt
0.003
1.7
rt
0.06
100.0
Day3
0:
06
0:
06:
50
i
0:
00:
35
0.154
I
0.004
1.6
I
0.08
94.1
0:
15
0:
14:
45
Ik
o:
oo:
oo
0.151
c
0.004
1.6
+
0.11
94.1
0:
30
0:
30:
00
i:
0:
01:
31
0.152
AZ
0.002
1.6
I
0.03
94.1
l:
oo
0:
59:
45
i
0:
00:
15
0.150
i:
0.003
1.9
+
0.28
111.8
Day5
0:
06
0:
06:
10
I
0:
00:
23
0.155
+
0.002
1.7
t
0.03
100.0
0:
15
0:
15:
20
I
0:
00:
31
0.154
of:
0.005
1.4
I!
0.07
82.4
0:
30
0:
30:
20
AZ
0:
01:
02
0.153
?I
0.005
1.6
­i:
0.02
94.1
l:
oo
1:
00:
00
rt
0:
00:
26
0.154
+
0.001
1.5
rt:
0.18
88.2
Stud
Averages
0.152
i
0.004
1.7
i
0.15
97.6
`Animals
were
dosed
on
a
5
day
schedule,
Body
Weight
and
Actual
Dose
are
for
the
day
of
sacrifice.
The
dose
administered
was
calculated
using
the
pre­
and
post­
dosing
syringe
weights
and
the
GC
analysis
of
the
dosing
solution.
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
32
of
69
Table
8.
Corn
Oil
Gavage:
Preliminary
study
to
determine
the
utility
of
MS/
MS
system.
Body
Weights,
Amounts
of
1,1,2­
TCE.

target
vol
syringe
syringe
lnj
Dose
Inj
Mouse
BW
(g)
(I4
pre­
weight
post­
weighi
(ml)
mg/
kg
1
22
220
5.199
4.944
0.254
28.91
2
23
230
5.156
4.905
0.251
27.25
3
23
230
5.140
4.915
0.225
24.42
4
24
240
Syringe
weights
not
recorded
5
23
230
5.132
4.896
0.236
25.67
Aw
23.0
26.6
SD
0.707
1.94
mg/
kg
Note:
The
administered
dose
is
averaged
from
animals
l­
3
and
5.
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
33
of
69
Table
9.
Female
BK3Fl
Blood:
Air
and
Female
F344
Rat
Brain:
air
and
Spleen:
air
Partition
Coefficients
Tissue
Partition
Coefficients
(mean
ir
S.
D)
Mouse
Blood
71.1
+
8.03
Rat
Spleen
43.0
i
8.16
Rat
Brain
56.1
+
4.94
Saline
11.7
+
2.07
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
34
of
69
APPENDIX
A.
Subchronic
inhalation
exposures
to
100
ppm
for
6
hr/
day
conducted
at
WIL
Laboratories
Table
A­
l
Oral
Gavage:
Female
BK3Fl
Mouse
390
mg/
kg
target
dose
in
corn
oil
Table
A­
2
Oral
Gavage:
Female
F344
Rat
92
mg/
kg
target
dose
in
corn
oil
Table
A­
3
Oral
Gavage:
Female
B6C3Fl
Mouse
10
mg/
kg
target
dose
in
water
Table
A­
4
Blood
concentrations
following
inhalation
exposures
in
mice
Table
A­
5
Blood
concentrations
following
inhalation
exposures
in
rats
B.
Individual
animal
data
for
gavage
studies
Table
B­
l
Oral
Gavage:
Female
B6C3Fl
Mouse
390
mg/
kg
target
dose
in
corn
oil
Table
B­
2
Oral
Gavage:
Female
F344
Rat
92
mg/
kg
target
dose
in
corn
oil
Table
B­
3
Oral
Gavage:
Female
B6C3Fl
Mouse
10
mg/
kg
target
dose
in
water
Table
B­
4
Oral
Gavage:
Female
F344
Rat
1.7
mg/
kg
target
dose
in
water
C.
Terminal
Tissue
Weights.

Table
C­
l.
Oral
Gavage:
Female
B6C3Fl
Mouse
390
mg/
kg
target
dose
in
corn
oil
Table
C­
2.
Oral
Gavage:
Female
F344
rat
92
mg/
kg
target
dose
in
corn
oil
Table
C­
3.
Oral
Gavage:
Female
B6C3Fl
Mouse
10
mg/
kg
target
dose
in
water
Table
C­
4.
Oral
Gavage:
Female
F344
rat
1.7
mg/
kg
target
dose
in
water
D.
Off­
Gassing
Study
Table
D­
l
Pilot
off­
gassing
study:
5
mice
were
treated
with
­25
mg/
kg
1,1,2­
TCE
via
gavage
in
corn
oil.

Table
D­
2
Exposure
and
Animal
information:
1000
ppm
Table
D­
3
Exposure
and
Animal
information:
500
ppm
Table
D­
4
Exposure
and
Animal
information:
250
ppm
Table
D­
5
Off­
gassing
of
1,1,2­
TCE
following
exposure
to
1000
ppm
by
inhalation
Table
D­
6
Off­
gassing
of
1,1,2­
TCE
following
exposure
to
500
ppm
by
inhalation
Table
D­
7
Off­
gassing
of
1,1,2­
TCE
following
exposure
to
250
ppm
by
inhalation
E.
Partition
Coefficients
Table
E­
l
Time
and
volume
determination
using
rat
blood
Table
E­
2
Blood:
Air
Partition
Coefficient:
Female
B6C3Fl
Mouse
Blood
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Page
35
of
69
Project
No.
11GO8
Appendix
A.
Subchronic
inhalation
exposures
to
100
ppm
for
6
hr/
day
conducted
at
VVIL
Laboratories
Table
A­
l.
Exposure
and
blood
collection
information
in
mice
and
rats
Day
1
­
­
_~­.­_­
I­­­_
­­
Exposure
Start
Exposure
End
Time
Animal
Removed­
Blood
Collection
Time
Mean
Cont.
Dody
Weight
­.~­_­_­

Animal
If
Group
#
/HH:
MM)
_­_
fHH:
MM)
/HH:
MM)
JHH:
MM)
.­.
Study
Davof
BW
Dateof
EtW
@pm)
(g)

69365
5
O&
16
15:
16
l3:
16
13:
lQ
99
156
OG125/
01
25
_.­_..
­
­­.­
~____
­______
__._
­.­
l_
.
69372
5
0B:
lG
15:
lG
13:
lG
13:
2G
99
­­­
159
0G/
25101
25
_I_­.
­­
69374
5
OR:
16
15:
16
­.­­­­­­
__
I­
13:
16
13:
21
99
166
06/
25/
01
25
­_­..­­
____
­.­
­_­­________­___­­._­.
69367
5
00~
16
15:
lG
1i:
lG
1520
99
`56
_____
._­
.I_­
25­­­
OG/
25/
01
____..
­.
____
­­­
69397
5
0616
15:
lG
15:
16
15:
20
99
IGD
06/
25/
01
25
­­­
­_­­~
­­­­
_I­­
~..
..­­.__
­­.­
G9400
5
0D:
lG
1516
15:
16
15:
20
99
171
OG/
25/
01
25
____
.­..­­
~
______
­_­.­.­_­.­".
69407
5
O&
16
_____
­
_______
II_^
__
..­
­­.­__
1516
15:
31
1535
99
155
OG/
25/
01
.­_­­­.
­___
__­­
25
­.­­­­
G9410
_­..
­­
.­_..._
­­__..__
5
O&
16
15:
lG
1531
1536
99
156
OGl25lOl
25
_
­.­..­
.___
_­­.­"
­
...____­
69420
5
08:
16
15:
16
15:
31
1535
99
I&
OG125/
01
25
~­­___­­
­
__­­­­
69429
5
O&
16
15:
16
1533
17:
16
99
­­­
K­­­
06/
25/
01
25
_­­­
­­
­___­­
G9430
5
0616
15:
16
1533
163
OF/
25/
01
25
__­­_.­___
­­­­
­
~­­­
I~.­.___­~_
­__
.._­
__
­.
.­­­­
GE)
434
5
__­
Of%:
16
15:
lG
15:
33
17:
18
99
170
06/
25/
01
25
~_.
.._
I.­­__­
~­.
____
­­­
­­.
­
­­­.
._­____­­..­
­­..­­.­
­
­
5207
G
07:
45
13:
45
II:
50
ll:
5G
100
­
2?
5­­
­
­
­
.­.­_­­
__^
­
OGf25l01
25
­­__
­__­
­­­.­
­­
­.­
­­
5212
G
07:
45
13:
45
II:
50
1154
100
24.1
OF/
25/
01
25
..­­
­­­­
­.­­
__
­­­.­
__
.­
­__­
5214
G
07:
45
13:
45
Ii:
50
II:
53
`00
.__­
22.2
OG/
25/
01
25
­_­.
­.
­___
5221
G
07145
13:
45
13:
4G
13:
50
100
22.5
OF/
25/
01
25
­­
­­­
_..­._
5224
___~.
___­_._
__
___.......
­.­
__­_.
­___
G
07:
45
13:
45
13:
46
13:
52
100
22.3
OF/
25/
01
25
._­­_
I_
­
~­­­.
_­
I
­__
__
_____
­I_.
­
.
.
.._
­­.
5225
6
07:
45
13:
45
13:
46
13:
55
100
21.7
OGf25fOl
25
.
­­
..­.
______
­..___.
____
­_..
__­­..­
5231
6
07:
45
13:
45
14:
03
14:
06
fQQ
_____­
­
..­
.
.._
­­__­
21.9
06f25lOl
25
­_­­­._
­­.
I__­­­­.____
5236
G
07:
45
13:
45
14:
03
14:&
i
100
22.1
OG/
25/
01
25
­_­­­
­­­­
­­
__­­
5238
6
07:
45
._____
­..._
.
­­.­­.­­_
13:
45
14:
03
14:
07
100
23.3
OGf2VOl
25
_­­­
.­
5239
6
07:
45
13:
45
14:
06
15:
45
100
23.3
06f25fOl
25
­
5245
F
07:
45
25
5249
G
­
.­­
.­..
__
I_
­..­
07:
45
13:
45
25
.­­
­­__

*
tt
should
bo
noted
that
2
minutes
were
subtracted
from
blood
collection
times
as
recorded
on
form
T5­
091­
5
for
this
study
!O
nCCOUnlfO1
a
discrepancy
of
1
minute
40
seconds
between
the
clock
used
for
exposure
time
and
that
used
in
necropsy.

l
It
should
also
be
noted
that
exposure
was
extended
for
1
hour
on
Group
5
to
account
for
problems
with
the
generation
system
for
that
group
that
occurred
near
the
beginning
of
exposure.
Concentrations
for
this
group
are
reported
as
a
lime
weighted
average
over
the
entire
exposure.
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
11GOU
Page
36
of
69
(Table
A­
l
Continued)

1­
69425
1
5
!
Of351
12:
51
­­
­­­­­
I
13:
06
I
13:
11
I
100
12:
51
13:
06
13:
ll
100
­_____

1320
13:
35
13:
39
`00
21.9
­­­
I
~­___­­
II_~~­­~­­
13:
20
13:
46
15:
21
100
23.8
­­~
13:
20
13:
46
15:
20
­­
­.­­.
100
23.6
_­
­
­__.­
13:
20
1
X46
1520
100
~­
%?~_~_­_
I)~!~!~!

l
It
should
be
noted
that
2
minutes
were
subtracted
from
blood
collection
times
as
recorded
on
form
T5­
091­
5
for
this
study
to
account
for
a
discrepancy
of
1
minute
40
seconds
between
the
clock
used
for
exposure
time
and
that
used
in
necropsy.
1,1,2­
trichlczthane:
PK
in
rats
and
mice
Project
No.
llCO8
(Table
A­
l.
Continued)

­­­

­__­_­

__
I­
­­­­­

69414
)
5
1
06:
54
__­.­­.
­­
__
­­

07:
21
­__­­­
0721
­
07~
21
5227
1
­."­_­
6
!
0721
"_
__
__

__
__

__

_.

­.

_.

__
Page
37
of
69
ExposureEnd
Time
Animal
Removed
Blood
Collection
Time
Mean
Cont.
Body
Weight
!B!
M!
a
iHH:
MM)
JHt­
kJMM_
I
f!­
lEEL.
Is).
­._­%.*
Study
DayofBJ
Date
of
BW
1254
IO:
54
10:
59
101
174
OtY25lOl
25
__
­­.­­­­
­­~
_______
I
12:
54
10:
54
IO:
59
101
178
06125101
25
­­­.
___.
12:
54
1054
1059
101
164
06/
25101
25
12:
54
12:
54
i2:
5a
101
158
06/
25/
01
25
~­.

­­
1254
I?:
54
12157
101
152
06/
25/
01
25
­­
_______
­.­­
12:
54
12;
54
12:
5a
101
164
OG125/
01
25
.­­­
___­.
12:
54
13:
09
13:
f2
101
137
06/
25/
01
25
­­­.
12:
54
13:
09
13:
12
101
170
OG/
25/
01
25
__..
­­.­
­­­__
~­
12:
54
13:
09
13:
12
101
­­
150
­­__­­_­
OG125fOl
25
1254
13:
ll
14157
101
161
OGl25fOl
25
.­­
.­
___­­
.­.­.­

14%
~­
.­

_ll­
13:
21
­­­
13:
21­
L­­­
13:
22
13:
25
­
­_
I­
I
'00
______
c
22.6
4
OBf25f01
25
­_
..­_
I
13:
21
13:
22
____..,
__
­­...­­
13:
21
IX22
­­­__.­
____
I_
­­___~­
13:
21
13:
36
___._
II
__.._
­­­.­_­_____

13:
21
13:
3G
­~~~­
­
_I_.._­­
­­­
13:
21
13:
36
­.­­
13:
21
13:
41
____­..
13:
21
13:
41
13:
21
13:
41
1,1,2­
trichiorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
38
of
69
Table
A­
2.
Blood
concentrations
following
inhalation
exposures
Samples
from
Wil
Labs
Limit
of
detection
0.013
pglg
B6C3Fl
Mouse
Inhalation
Exposures,
Data
collected
June
2529,290l
Collection
_­~
~..~
.­
.____,___
~.
._
.
.
­.­..­­..
.
Date
Sample
Duplicates
1
25Jun
Time
blood
cone
(ug/
g)
Average
for
Timepoint
An#
hr
a
b
avg
AVG
SD
j
5207
5.00
:
0.639
0.57
0.6045
0.608
0.014
j
5212
0.572
0.621
0.5965
j
5214
0.623
0.623
/
5221
7.00
!
1.121
0.97
1.0455
0.951
0.083
i
5224
0.852
0.979
0.9155
I
/
5225
0.859
0.922
0.8905
5231
7.25
1.174
1.289
1.2315
0.960
0.243
5236
0.807
0.722
0.7645
;
5238
0.783
0.984
0.8835
I
5239
9.00
0.047
0.015
0.031
0.022
0.009
/
5245
0.012
0.014
0.013
i
5249
0.024
0.022
0.023
._._
­
..__
­­­
_....
..__
Collection
­­
Date
Sample
Duplicates
27­
Jun
Time
1
blood
cone
&g/
g)
verage
for
Timepoint
An#
hr
'
a
b
avg
1
AVG
SD
5209
4.0
:
2.08
2.02
2.05
j
1.838
0.277
5215
5216
5217
5218
5220
5222
5226
5229
5234
5247
5248
1.5
1.55
1.525
1.84
2.04
1.94
6.0
1.89
2.18
2.035
1.728
0.368
1.8
1.86
1.83
1.11
1.53
1.32
6.25
;
0.53
0.58
0.555
0.433
0.107
I
0.39
0.39
0.39
0.41
0.3
0.355
8.0
ND
#DIV/
O!
#DIV/
O!
#DIV/
O!

8.00
ND
$DIV/
O!

8.00
0
0
_____
­­­­
.­
­
.­­­­
.
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
39
of
69
(Table
A­
2
Continued)

Collection
____~_______
_­­
Date
29Jun
Time!
Sample
Duplicates
An#
hr
/
blood
cone
(pg/
g)
Average
for
Timepoint
a
b
j
avg
AVG
SD
5204
4.0
j
1.98
2.29
2.135
1.983
0.167
5205
I
1.78
1.83
1.805
1.77
2.25
2.01
2.33
2.74
j
2.535
'
2.162
0.368
2.15
2.15
2.15
0.67
0.705
0.592
0.098
1
5232
'
0.54
0.53
0.535
/

5235
a.
0
i
0.04
0.04
:
0.04
0.055
0.015
5240
8.00
;
0.07
0.07
j
0.07
5242
a.
00
i
0.06
0.05
0.055
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
40
of
69
Table
A­
3.
Blood
concentrations
following
inhalation
exposures
in
rats
F344
RAT
inhalation
Exposures,
Data
collected
June
25­
29,
2001
Collection
r
.
.
.
­..­­.
.._
.._.
­_­__­.
.
.
.
.
.
Date
Sample
Duplicates
25Jun
Time,
blood
cone
(pglg)
Average
for
Timepoint
An#
hr
:
a
b
avg
AVG
SD
69365
5.00'
1.87
2.29
2.08
1.997
0.101
69372
2.01
1.76
i
1.885
j
69374
1
2.03
2.02
!
2.025
1
69387
2.6
2.63
2.615
2.337
0.345
j
69397
7.00;

!
2.33
2.56
2.445
i
69400
:
2
1.9
1.95
1
7.25
/
j
69407
1.016
0.964
0.936
0.047
69410
I
0.89
0.923
0.9065
0.99
1
69420
$
0.927
0.893
0.91
1
69429
9.00;
0.122
/
69430
j
0.168
i
69434
I
0.105
Collection
Date
/

27­
Jun
Time/
Sample
Duplicates
Anf:
hrj
blood
cone
@g/
g)
duplicate
Average
for
Timepoint
a
b
:
avg
AVG
SD
j
69357
4.0
j
1.84
I.
8
1.82
1.777
0.051
j
69358
i
1.74
1.7
;
1.72
j
69360
:
1.9
1.68
1.79
i
69399
6.0
j
1.79
2.38
2.085
1.738
0.355
/
69403
I
1.77
1.74
1.755
i
69411
1.41
1.34
1.375
!
69418
j
69421
6.25;
0.64
0.55
!
0.33
0.42
0.595
0.375
0.533
0.138
I
69422
0.53
0.73
0.63
j
69423
8.0
j
0.13
E5
L
0.108
0.058
I
69424
b425
1
0.05
i
0.12
._.­
____
­­_..
___
­...
0.;.
1
1
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
41
of
69
(Table
A­
3
Continued)

Collection
1,1,2­
trichL,
ethane:
PK
in
rats
and
mice
Project
No.
41608
Page
42
of
69
Appendix
B.
Individual
Animal
Data
from
Demonstration
of
Periodicity
Following
Repeated
Gavage
Administrations.

B­
l.
Mouse
Corn
Oil
Gavage
Day
1
1
.I
,2TCE
cont.

Sample
Duplicates
1
,I
,2­
TCE
AVG
AVG
BNW­
MO01
19.2
15.350
19.5
0.57
354.3
12.48
BNW­
MO02
20.2
341.4
31
BNW­
MO03
19.2
366.3
33
­.­­._­­­.­
___­..
BNW­
MO04
19.3
343.1
60
BNW­
MO05
19.7
359.7
58
BNW­
MOO6
20.5
353.9
58
12.430
­­­­­_­
.­.­__.
BNW­
MO07
19.5
BNW­
MO08
19.7
2.820
BNW­
MOO9
20.7
BNW­
MO10
19.5
BNW­
MO11
20.6
BNW­
MO12
20.5
­______­­.____

lay
3
`&&
V­
M013
19.1
364.9
30
BNW­
MO14
20.2
343.2
32
BNW­
MO15
20.4
354.4
35
­__­~­­­­
BNW­
MO1
G
18.9
428.6
Ei8
BNW­
MO17
18.2
399.8
58
BNW­
MO18
20.2
344.8
61
BNW­
MO19
20.0
327.1
120
BNW­
MO20
19.1
391
.o
120
BNW­
MO21
20.3
362.5
123
__­­­­­~~­
BNW­
MO22
18.4
375.8
478
BNW­
MO23
18.5
377.5
477
BNW­
MO24
19.2
361.7
475
Table
B­
l
Continued
0.50
17.005
17.077­

0.53
21.599
20.153
0
58
14.168
­­­
A­­
­­
13.032
0.97
11.560
14.450
0.97
26.040
26.020
1.02
22.110
21.660
2.00
18.300
16.440
2.00
20.990
19.120
2.05
18.450
21.330
7.97
7.210
8.120
7.95
10.990
7.92
7.010
6.680
C
_~­
AVG
BW
(9)
SD
­­­­
.~
17.041
13.9
0.70
20.876
13.600
13.005
19.1
1.02
26.030
21.885
17.370
19.8
0.63
20.055
19.890
­­
7.665
18.7
0.44
10.990
6.845
AVG
AVG
`0~
Q­%$
kgl_
S_
D
E!!
z.(
hi
­
­
­.
354.2
10.85
0.54
­­.___
391.1
42.58
0.98
__­
3G0.2
32.01
2.02
­­
371.7
8.67
7.94
AVG
AVG
AVG
timepoint
AVG
Time
(hr)
AVG
(pg/
g)
SD
0.52
11.132
4.577
.­­
0.98
7.837
3.991
8.00
0.154
I
1
.I
,2TCE
cont.

imepoint
AVG
`VG
(Kl!
cL2L
­­
17.172
3.640
20.307
6.654
­­­____
19.105
1.505
8.500
2.195
1
,I
.2TCE
cont.

AVG
timepoint
AVG
1,1,2­
trichlorcthane:
PK
in
rats
and
mice
Project
No.
11608
Page
13
of
69
Day
5
_­.
­.­
BEls).­
sD­~~~[
n_
g&
Q)>
P~
Tim_
e_
LUAVG(
Clg~~
BNW­
MO25
17.7
372.9
30
19.2
1.45
365.3
16.16
0.52
25.013
10.805
BNW­
M02F
19.3
376.2
30
23.430
24.090
23.760
.­__.­­­
­­­_
19.0
0.57
399.2
31.30
0.98
18.020
4.876
BNW­
MO29
19.5
363.2
59
16.500
BNW­
M03u
19.2
BNW­
MO31
18.2
19.0
0.71
378.3
48.19
2.02
23.462
2.136
BNW­
MO32
19.1
BNW­
MO33
19.6
.­­­­­
__­
_I____
BNW­
MO34
18.1
17.8
0.64
358.1
18.13
8.03
12.022
2.577
BNW­
MO35
17.1
BNW­
MO36
18.3
NJ
19.3
365.5
SD
0.91
25.8
]
93.72%
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
44
of
69
B­
2.
Rat
Corn
Oil
Gavage
Day
1
An
ft
­_­_
BW
&L
.­
BNW­
ROOI
148.2
BNW­
R002
140.9
BNW­
R003
146
6
..­­_­­­­­­­~_
BNW­
R004
146.8
BNW­
R005
141
.O
BNW­
ROO6
145.5
_I_­
­­
BNW­
R007
147.6
BNW­
ROCEI
139.1
Dose
Time
iE!
J!
kL~~­
97.6
34
98.3
33
96.6
33
94.3
50
_­

97.4
60
­95.4
61
96.0
122
98.8
122
BNW­
R009
148.4
93.6
120
­.­.­­__
BNW­
ROIO
152.0
93.5
479
BNW­
ROI
1
146.5
99.6
482
BNW­
R012
149.8
98.0
482
­­­­

klv
3
BNW­
R013
145.5
91.3
29
BNW­
R014
153.1
101.3
28
BNW­
ROf
5
144.3
96
2
­­­
­­­
2
34
BNW­
ROI
G
146.6
94.6
59
BNW­
ROI
7
154.5
88.1
61
BNW­
R018
142.9
95.7
62
­
­­­­­­
­.­­­_____
BNW­
RO19
145.2
94.5
120
BNW­
R020
150.2
90.9
121
BNW­
R02
1
144.0
94.8
123
­.
._­_"
­_­.­.­_
­­­~__­.._.­
BNW­
R022
147.2
91.1
479
BNW­
R023
146.0
92.9
482
BNW­
R024
148.6
101.3
482
­­
___­
Table
B­
2
Continued
­^­.­^..­.
Tirnc
Sample
Duplicate.

(hrJ
A
B
­
I
I_­____~
0.567
0.550
0
550
­.­­
L­­­­
0.833
1
.ooo
1.017
2.033
2.033
2.000
7.983
8.033
a
033
­­
L_­"__

0.483
0.467
0.567
­­
0.983
1.017
1.033
__
I.
2.000
2.017
2.050
­­­­
7.983
8.033
8.033
­­

­­

­­

­­
15.73
18.09
16.33
16.51
J
6.52
16.19
9.06
9.00
11.52
12.72
9.97
9.70
5.20
5.44
5.56
5.10
5.98
6.73
0.23
0.20
0.15
0.15
0.11
0.12
6.42
5.92
9.74
10.79
12.82
12.36
5.62
5.30
6.92
2.56
8.56
9
10
.

2.53
2.58
1.77
1.87
2.11
2.32
­0.05
0.05
0.04
0.04
0.04
0.08
­­

­­

­­

l­

­­

_­

­­
1
,I
,2TCE
cont.

,I
,2­
TCE
AVG
AVG
AVG
timepoint
AVG
BW
(9)
SD
Dose
(mglkg)
SD
Time
(hr)
AVG
&g/
g)
Avg
SD
16.910
145.2
3.84
97.5
0.85
0.56
16.562
0.303
16.420
106.0%

%&/­%
i?(,,
0.95
10.328
1.603
9.835
1
5.320
1
145.0
5.35
1~
1­?%?­­/
5.668
0.595
5.330
104.5%

6.355
0.211
149.2~
2.77
97.0
3.16
8.02
0.158
0.050
0.150
105.5%

0.113
­­
___­

AVG
AVG
AVG
6.170
BW
(91..
14%
SD
DoseJmg/
kg)
SD
Time
(hr
4.77
96.3
5.00
0.51
10.265
104.6%

12.590
­­
__­
­
­­.
.­­­
5.460
14a.
o
5.93
92.8
4.11
1
.Ol
4.740
100.9%
8.830
­­­___
2.555
146.5
3.29
93.4
2.17
2.02
1.820
101
.5%
2.215
­6.050
­__­.
147.3
1.30
95.1
5.44
8.02
0.040
103.4%

0.063
1
2;

­­

­­

­1
,I
,2TCE
cont.
`mepoint
AVG
\LG
(i&
d
­­
._
SD
8.478
3.250
8.122
2.183
2.197
0.368
0.051
0.012
,I
,2TCE
cont.
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
11608
!z!!
5
.­­­.­
BNW­
R025
150.9
91.1
29
BNW­
R02G
144.8
94.6
31
BNW­
R027
151
0
93.4
32
__­­
­­­.
L­­
­­
BNW­
R028
155.7
88.4
63
BNW­
R029
142.5
93.9
62
BNW­
R030
142.9
96.8
65
BNW­
R031
155.0
88.6
118
BNW­
R032
143.6
93.9
120
BNW­
R033
150.6
91.3
122
BNW­
R034
143.9
92.3
482
BNW­
R035
149.1
93.3
479
BNW­
R036
156.7
84.4
482
Av9
147.422
94.244
I
0.483
0.517
0.533
­­­
1.050
1.033
1.083
1.967
2.000
2.033
8.033
7.983
8.033
~­
12.89
13.97
10.54
10.53
6.28
6.66
7.56
7.94
8.84
9.48
7.96
10.25
4.17
4.17
3.16
3.01
4.46
6.47
0.17
0.15
0.05
0.05
0.68
0.60
SD
4.342
3.719
/102.44%
1
I
13.430
10.535
6.470
7.750
9.160
9.105
­­­
4.170
3.085
5.465
0.160
0.048
0.640
Page
45
of
69
AVG
AVG
BW
(4
SD
Dose
mglkg)
SD
­­._
­
148.9
­­
l­­
­­­
3.55
93.0
1.78
101.­
l%

­..
147.0
7.51
93.0
4.27
101.1%

149.7
5.75
91.3
2.65
99.2%

­­­
149.9
6.44
89.7
4.56
97.5%
1
­_

­­

_­

­­
1.192
8.02
­­­
I
0.283
0.315
I
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Page
46
of
69
Project
No.
11608
B­
3.
Mouse
Water
Gavage
Day
1
r­

f
Dose
Time
\n
#
fE!!~
Um_
sLks~~~
E~
BNW­
Ml01
19.4
9.0
6.0
BNW­
Ml02
17.9
10.1
6.0
BNW­
Ml03
18.1
10.3
6.3
BNW­
Ml04
18.2
10.3
14.5
BNW­
MI05
18.3
10.7
14.0
BNW­
Ml06
20.3
8.9
14.5
.­­­­
BNW­
Ml07
20.1
8.8
30.5
BNW­
Ml08
20.0
9.6
29.8
BNW­
Ml09
18.8
102
30
3
­­­­­­­*.^
A
BNW­
Ml10
20.4
9.4
59.3
BNW­
Ml11
19.8
9.4
60.3
BNW­
Ml12
18.1
9.9
59.5
­­

33
BNW­
MI
13
18.8
9.9
6.0
BNW­
Ml14
19.4
9.4
6.8
BNW­
Ml15
18.8
9.6
8.2
BNW­
Ml16
18.1
11.5
14.5
BNW­
MI
17
18.5
8.1
14.3
BNW­
Ml18
19.2
12.1
15.3
~..
__­
BNW­
Ml19
18.4
13.0
31.8
BNW­
Ml20
18.9
10.2
30.3
BNW­
Ml21
19.1
9.1
30.8
%W­
Ml22
­__
19.2
8.7
59.5
BNW­
Ml;;
19.0
8.6
59.8
BNW­
Ml24
18.9
11.2
60.0
l%&
e
B­
3
Continued
­_.__­­
Time
Sample
Duplicates
1
,I
,2­
TCE
AVG
BW
(9)
SD
18.5
0.81
0.104
0.306
0.296
0.301
0.242
b.
270
0.270
18.9
1.18
0.233
0.201
0.198
0.200
0.242
0.182
0.182
0.508
0.130
0.090
0.110
19.6
0.72
0.496
0.168
Oll68
0.504
0.182
0.182
­­
A_
­._­
0.988
0.064
0.064
­19.4
1.19
1.004
0.034
0.026
0.030
0.992
0.030
0.027
0.029
AVG
BW
@>
SD
0.310
0.290
0.300
19.0
0.35
0.086
0.103
0.095
0.110
0.090
0.100
0.033
0.037
18.6
0.56
0.030
0.031
0.062
0.047
­­
­
0.016
0.016
18.8
0.36
0.054
0.063
0.020
0.018
0.017
0.016
19.0
0.15
0.026
nd
C
c
_­
AVG
lose
(mglkg)
SD
9.8
0.70
10.0
0.92
9.5
0.73
9.6
0.29
AVG
lose
(mglkg)
SD
9.6
0.26
10.6
2.18
10.7
2.03
9.5
1.45
0.99
0.041
0.020
­
1
,I
.2TCE
cont.
AVG
timepoint
AVG
E~~.~!
O­
AVGP~~
SD
0.12
0.165
0.117
­­
0.24
0.038
0.008
0.52
0.032
0.026
­___.
1
.oo
0.02
1
0.007
1
.I
.2TCE
cont.
1,1,2­
trichlnwthane:
PK
in
rats
and
mice
Project
No.
11608
Page
47
of
69
`35
­
BNW­
Ml25
20.8
9.6
6.5
BNW­
Ml26
20.2
9.4
7.0
BNW­
Ml27
18.8
9.8
6.0
BNW­
Ml28
20.4
9.4
16.0
BNW­
Ml29
20.1
9.6
15.5
BNW­
Ml30
20.3
6.5
158
.­­
L
BNW­
Ml31
21.5
8.7
30.3
BNW­
Ml32
19.7
10.1
31.3
BNW­
M
133
20.4
9.1
30.3
BNW­
Ml34
20.9
8.7
60.3
BNW­
M135,
20.1
9.7
60.3
BNW­
Ml36
19.2
9.6
61.0
Avg
19.4
9.7
SD
0.9
1.1
­­­
0.108
0.117
0.100
0.267
0.258
0.263
­.­­..­
0.504
0.521
0.504
1.004
1.004
1.017
0.290
0.210
0.130
0.110
0
270
­L­­.
0.280
0.049
0.044
0.039
0.032
0.040
0.100
­­­
0.089
0.093
0.065
0.034
0.030
0.025
0.024
0.040
0
027
­2
0.026
0.250
0.120
0
275
­­.­
L
0.047
0.036
0.070
0.089
0.079
0.032
0.025
0.040
2.027
AVG
AVG
BW_!
g)­
SD
Dose
(mg/
kq)
SD
­­.­
a­
5.
19.9
1.03
9.6
0.20
20.3
0.15
8.5
1.74
2o.
F
0.91
9.3
0.75
20.1
0.85
9.3
0.55
0.26
0.051
0.018
0.51
0.067
0.030
1.01
0.030
0.008
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
B­
4.
Rat
Water
Gavage
Day
1
Dose
Time
A!!
ff
BW
~~­­@~~~~~~
BNW­
RI01
147.8
1.69
6.3
BNW­
RI02
146.9
1.70
6.0
BNW­
RI03
148.4
1.70
6.3
.­­
BNW­
RI
04
144.6
1.73
~­­
15.3
BNW­
RI05
146.3
1.84
14.3
BNW­
RIO6
148
8
1
67
­­­­­
A
15.0
.BNW­
RI
07
150.2
1.70
30.3
BNW­
RI
08
146.9
1.71
31.0
BNW­
RI
09
150.3
1.65
31.5
_­__­­
BNW­
RI10
151.6
1.60
60.3
BNW­
RI
11
148.3
1.72
59.3
BNW­
RI12
151.4
1.68
GO.
0
­~~.­­_.­­
.~
__­__

33
BNW­
RI
13
150.7
1.69
7.5
BNW­
RI
14
158.4
1.54
6.5
BNW­
RI
15
151.8
1.68
6.5
­­­
BNW­
RI
16
146.6
1.75
14.8
BNW­
RI
17
153.4
1
55
14.8
BNW­
RI
18
152.6
1:
59
14.8
BNW­
RI
19
150.1
1.61
28.3
BNW­
RI20
154.2
1.66
31
.o
BNW­
RI21
151.6
1.64
30.8
BNW­
RI22
151.5
1.80
59.8
BNW­
R123
146.3
1.68
60.3
BNW­
RI24
151.2
2.22
,
60.0
Table
B­
4
Continued
Time
­@!
L
0.104
0.100
0
104
­L­­­
0.254
0.238
0.250
___­
0.504
0.517
0
525
­­.
L­_­­
1.004
0.988
1
000
L­..­­
2amplc
Duplicates
1
,I
,2­
TCE
A
B
Avg
­_­
0.047
0.030
0.039
0.038
0.037
0.038
JO32
0.039
0.036
­_
l_
0.020
0.020
0.020
0.015
0.014
0.015
0.030
0.060
0.045
­­
.­
0.020
0.020
0.020
0.041
0.021
0.031
0.022
0.017
0.020
~­_.
~­
­­
0.014
0.014
0.014
0.020
0.010
0.015
0.029
0.020
0
025
L­

0.125
0.043
0.047
0.045
0.108
0.028
0.031
0.030
0.108
0.037
0.040
0.039
0.246
0.014
0.014
0.246
0.018
0.018
0.246
0.050
0.030
0.040
0.471
0.020
0.010
0.015
0.517
0.015
0.015
0.513
0.041
0.053
0.047
0.996
0.052
0.037
0.045
1.004
0.027
0.027
0.027
1
.ooo
0.014
0.013
0.014
PXJC
48
of
69
AVG
AVG
SD
BW
(g)
lose
(mglkg)
SD
147.7
0.75
1.7
0.01
146.6
2.11
149.1
1.93
150.4
1.85
1.7
0.06
AVG
AVG
sw((?)
so~
lose
(mg/
kg)
SD
153.6
4.16
1.6
0.08
150.9
3.72
1
.G
0.11
152.0
2.07
l.
G
0.03
­149.7
2.92
1.9
0.28
­
1.7
0.09
1.7
0.03
­I
­

­.

_

­­

"
I
­­
1
,I
,2TCE
cont.

AVG
timepoint
AVG
Time
(hr)
AVG
(pglg)
SD
0.10
0.037
0.00;

0.25
0.027
0.01
E
0.52
0.024
0.006
­~
1
.oo
0.018
0.006
1
,I
,2TCE
cont.

AVG
timepoint
AVG
Time
(hr)
AVG
&g/
g)
SD
0.11
0.038
0.008
­.­__.­­
__
0.25
0.024
0.014
0.50
0.026
0.018
1
.oo
0.028
0.01
G
1
,I
,2TCE
cont.
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
3ay
5
._
___
..­_
BNW­
RI25
153.7
BNW­
RI26
154.0
1.76
6.3
1.71
5.8
BNW­
R127
157.6
1.73
G.
5
­­­
BNW­
RI
28
150.3
1.49
15.5
BNW­
RI29
159.1
1.34
15.8
BNW­
RI30
151
6
1
45
14.8
__^___
­
.____
­_..­..­.­...
A­­
­:­.
..­­­­­
BNW­
RI
31
156.3
1.63
29.5
BNW­
R132
156.7
1.65
31.5
BNW­
RI33
147
1
­­_­­­­
L
1.67
30.0
BNW­
RI
34
163.9
1.54
59.8
BNW­
RI
35
152.0
1.33
60.5
BNW­
RI36
154
2
__
p­..­­­.­
2
1.68
59.8
AxI
151.6
1.66
SD
4.2
0.15
0.104
0.068
0.079
0.096
0.074
0.079
0.108
0.047
0.041
0.258
0.028
0,024
0.263
0.027
0.038
OmL
3027
__
0.492
0.013
0.013
0.525
0.038
0.016
0.500
0.014
0.046
­
0.996
0.016
0.046
1.008
0.014
­2.­
0
996
.­­
I­
0
031
0.074
0.077
0.044
0.026
0.033
0.027
­._____
0.013
0.027
0.030
0.031
0.014
0.031
_­_
Page
49
of
69
AVG
AVG
BW
(n)
­_.
.._
SD
Dose
(mglkq)
SD
­­­­.­
5
.­­­
155.1
2.17
1.7
0.03
153.7
4.75
1.4
0.08
153.4
5.43
1.7
0.02
156.7
6.33
1.5
0.18
AVG
timepoint
AVG
Time
(hr)
AVG
@g/
g)
..­­
SD
0.10
0.065
0.018
0.26
0.029
0.004
0.51
0.023
0.009
­7.00
0.025
0.010
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
50
of
69
Appendix
C.
Terminal
Tissue
Weights.

Table
C­
l.
Oral
Gavage:
Female
B6C3Fl
Mouse
390
mg/
kg
target
dose
in
corn
oil
Scheduled
Sacrifice(
hr)
Lung
W(
g)
Liver
wt.
(q)
Kidney
wt.
(q)
Spleen
wt.
(g)
Brain
wt.
(q)

Day1
0.5
0.177
I
0.008
1.05
~fr
0.061
0.282
10.005
0.079
5
0.003
0.464
i
0.014
1
0.180
2
0.021
1.03
?I
0.089
,0.290
e
0.031
0.076
f
0.005
0.468
zk
0.011
2
0.178
+
0.001
0.99
+
0.065
0.300+
0.013
0.070
i
0.002
0.475
5
0.002
8
0.163
i
0.013
0.94
i
0.082
0.270
i
0.003
0.068
I
0.001
0.466
i
0.014
Day
3
0.5
0.170
5
0.031
1.17
i
0.115
0.276
k
0.005
0.083
t
0.003
0.474
+
0.013
1
0.165
f
0.014
1.00
f
0.111
0.275
f
0.009
0.069
k
0.002
0.471
f
0.005
2
0.167
i
0.002
1.09
i
0.061
0.284
Z!
I
0.019
0.064
it
0.004
0.469
I!
Z
0.014
8
0.163
+­
0.013
0.94
f
0.082
0.270
i
0.003
0.068
i:
0.001
0.466
+
0.014
Day
5
0.5
0.184
i
0.046
1.34
i
0.155
0.283
­i
0.015
0.066
k
0.015
0.467
2
0.004
1
0.154
f
0.017
1.38
+
0.015
0.273
i
0.002
0.068
2
0.003
0.455
i
0.006
2
0.154
f
0.012
1.26
i
0.081
0.284
i
0.004
0.071&
0.013
0.460
f
0.001
8
0.151+
0.008
1.22
i:
0.078
0.255
i
0.056
0.073
c
0.01
0.449
f
0.010
1,1,2­
trichlorethane:
PKin
rats
and
mice
Page
51of69
Project
No.
41608
Table
C­
2.
Oral
Gavage:
Female
F344
Rat
92
mg/
kg
target
dose
in
corn
oil
Scheduled
Sacrifice
(hr)
Lunq
wt(
g)
Liverwt.
(q)
Kidney
wt.(
q)
Spleen
wt.
(q)
Brain
wt.(
q)
Day1
0.5
0.731
5
0.047
4.89
i
0.385
1.11
f
0.034
0.3912
0.017
1.67
4
0.047
1
0.678
2
0.019
5.04
i
0.155
1.12
I
0.068
0.370
5
0.030
1.66
I
0.037
2
0.715
10.035
4.92
?I
0.307
1.09
10.078
0,367
2
0.014
1.65
t
0.042
8
0.737
I
0.032
4.71
iz
0.317
1.10
F
0.054
0,352
i
0.018
1.62
k
0.033
Day3
0.5
0.698
t
0.023
5.21
f
0.131
1.11
­t
0,054
0,377
+
0.015
lo62
k
0.050
1
0.7312
0.070
5.16
zk
0.293
1.18
I
0.016
0.365
+
0.038
1.67
k
0.046
'2
0.721
f
0.018
5.04
f
0.081
1.18
rt
0.038
0.355
?c
0.012
1.67
f
0.047
8
0.739
+
0.052
4.70
k
0.211
1.15
+
0.059
0.340
f
0.008
1.63
I
0.051
Day
5
0.5
0.723
i
0.010
5.19
+
0.217
1.17
i
0.059
0.345
k
0.009
1.63
1.
0.011
1
0.726
i
0.031
5.16
1.
0.345
1.19
k
0.029
0.360
i
0.016
1.62
f
0.041
2
0.715
f
0.009
5.13
+
0.218
1.15
i
0,014
0.349
k
0.008
1.68
+
0.032
8
O­
741?
0.037
5.00
10.113
1.19
IO.
075
0.348
I
0.027
1.62
f
0.019
1,1,2+
richlorethane:
PK
in
rats
and
mice
Page
52
of
69
Project
No.
41608
Table
C­
3.
Oral
Gavage:
Female
66C3Fl
Mouse
10
mg/
kg
target
dose
in
water
Scheduled
Sacrifice
(hr)
Lunq
wt
@)
Liver
wt.
(q)
Kidneywt,
(g)
Spleen
wt.
(g)
Brain
wt.
(g)
Day
1
0.1
0.137
rt
0.028
0.929
It
0.049
0.249
rt
0,002
0.059
IO.
006
0.437
i
0.034
0.25
0.138
i
0.010
0.909
i
0.097
0.243
I
0.007
0.063
I
0.006
0.440
rt
0.011
0.5
0.134
k
0.010
1.008
i
0.040
0,251~
0.015
0.062
i
0.002
0.436
+
0.005
1
O.
l31_
t
0.012
0.940
5
0.054
0.239
F
0.015
0.058
+
0.009
0.429
+
0.017
Day3
0.1
0.149
k
0.023
0.976
5
0.075
0.266
rt
0.016
0.059
2
0.003
0.457
F
0.006
0.25
0.165
k
0.041
1.098
2
0.359
0.244
k
0.014
0.057
i
0.003
0.433
f
0.005
0.5
0.137
?r:
0.014
0.946
i
0.076
0.245
i:
0.006
0.063
+
0.009
0.437
+
0.008
1
0,157
k
0.027
0.888
rt
0.093
0.244i
0.012
0.059
i.
0.000
0.431_+
0.031
Day5
0.1
0.141
f
0.017
1.024
I
00088
0.250
k
0.009
0.057
+
0.009
0.451
+
0.018
0.25
0.134
t
0.016
0.931
ri:
0.051
0.264
i
0.006
0.057
i:
0.005
0.440
5
0.004
0.5
0.134
f
0.018
0.9911
0.060
0.277
2
0.005
0.063
F
0.009
0.447
I
0.012
1
0.181
i­
0.049
0.940
i
0.029
0.255
+
0.009
0.062
I
0.010
0.444
f
0.012
1,1,2­
trichlorethane:
PKin
rats
and
mice
Page
53
of69
Project
No.
41608
Table
C­
4.
Oral
Gavage:
Female
F344
Rat
1.7
mg/
kg
target
dose
in
water
Scheduled
Sacrifice
(hr)
Lung
wt(
q)
Liverwt.
(g)
Kidneywt.
(q)
Spleen
wt.(
g)
Brain
wt.(
q)
Day
1
0.1
0.685
5
0.024
5.29
k
0.046
1.23
f
0.19
0.362
t
0.008
1.63
2
0.031
0.25
0.744
4
0.044
5.27
i
0.041
1.14
i
0.037
0.376
I
0.006
1.61
I
0.019
0.5
0.793
k
0.060
5.59
i
0.267
1.17
t
0.11
0.365
2
0.026
1.59
+
0.071
1
0.741
+
0.019
5.23
i
0.052
1.17
+
0.044
0.364
rt
0.009
1.61
f:
0.023
Day3
0.1
0.811
k
0.065
5.64
i.
0.318
1.23
t
0.070
0.373
z!
z
0.021
1.67
%
0.022
0.25
0.879
zk
0.080
5.31
i
0.296
1.26
t
0.035
0.380
i
0.016
1.69
5
0.024
0.5
0.804
zk
0.119
5.33
+
0.061
1.20
k
0.063
0,357
5
0,030
1.66
f
0.007
1
0.825
+
0.001
5.43
f
0.423
1.16
k
0.07
0.363
i
0.013
1.69
2
0.014
Day
5
0.1
0.817
zk
0.056
5.60
+
0.375
1.22
+
0.021
0.374
IO.
011
1.71
*
0.010
0.25
0.860
5
0.072
5.75
k
0.159
1.25
i
0.046
0.365
2
0.012
1.66
I
0.058
0.5
0.790
2
0.019
5.45
10.056
1.23
2
0.030
0.379
5
0.013
1.70
i:
0.009
1
0.810
f
0.055
5.75
i
0.454
1.25
i
0.045
0.380
z
0.011
1.68
=
0.005
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Appendix
D.
Off­
Gassing
Data
Table
p­
1.
Pilot
study
for
off­
gassing
of
1,1,2­
TCE
Time
Post
Exposure
(hr)
0.19
0.20
0.22
0.24
0.25
0.27
0.29
0.30
0.32
0.34
0.35
0.37
0.39
0.40
0.42
0.44
0.45
0.47
0.49
0.50
0.52
0.54
0.55
0.57
0.59
0.60
0.62
0.64
0.65
0.67
0.69
0.70
0.72
0.74
0.76
Chamber
Cone,
(wb)
315.6
473.9
524.3
563.0
497.1
522.3
548.8
679.0
661.4
687.5
630.7
646.3
622.3
614.1
604.1
595.5
658.5
600.2
567.1
567.1
526.9
582.6
602.9
536.6
528.1
549.0
490.9
374.2
328.3
362.7
325.2
262.8
243.0
228.9
253.5
Time
Post
Exposure
(hr)
0.77
0.79
0.81
0.82
0.84
0.86
0.87
0.89
0.91
0.92
0.94
0.96
0.97
0.99
1.01
1.02
1.04
1.06
1.07
1.09
I,
11
1.12
1.14
1.16
1.17
1.19
1.21
1.22
1.24
1.26
1.27
1,29
1.31
1.32
1.34
Chamber
Cont.
(wb)
255.1
271.6
253.6
186.4
188.7
243.3
227.3
258.3
212.5
192.1
170.6
176.4
225.9
204.6
73.5
43.9
153.3
126.8
93.7
80.5
44.8
97.3
90.9
57.8
87.1
40.6
1.8
47.4
48.3
72,4
28.1
30.1
12.8
45.0
91.9
Page
54
of
69
Time
Post
Exposure
(hr)
1.36
1.37
1.39
1.41
1.43
1.44
1.46
1.48
1.49
1.51
.
1.53
1.54
1.56
1.58
1.59
1.61
1.63
1.64
1.66
1.68
1.69
1.71
1.73
1.74
1.76
1.78
1.79
1.81
1.83
1.84
1.86
1.88
1.89
1.91
1.93
Chamber
Cont.
cm&

8.;
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
.
0.0
0.0
0.0
0.0
0.0
Page
55
of
69
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Time
Post
Exposure
(hr)
1.94
1.96
1.98
1.99
2.01
2.03
2.04
2.06
2.08
2.10
2.11
2.13
2.15
2.16
2.18
2.20
2.21
2.23
2.25
2.26
2.28
2.30
2.31
2.33
2.35
2.36
2.38
2.40
2.41
2.43
2.45
2.46
Chamber
COIX.
(ppb)
­0l0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
Chamber
Time
Post
Cont.
Exposure
(hr)
(ppb)
2.48
2.50
2.51
2.53
2.55
2.56
2.58
2.60
2.61
2.63
2.65
2.66
2.68
2.70
2.71
2.73
2.75
2.77
2.78
2.80
2.82
2.83
2.85
2.87
2.88
2.90
2.92
2.93
2,95
2.97
2.98
3.00
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
Time
Post
Exposure
(hr)
3.02
3.03
3.05
3.07
3.08
3.10
3.12
3.13
3.15
3.17
3.18
3.20
3.22
3.23
3.25
3.27
3.28
3.30
3.32
3.33
3.35
3.37
3.38
3.40
3.42
3.44
3.45
3.47
3.49
3.50
Chamber
Cont.

(;
P;)

0:
o
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
56
of
69
Table
D­
2.
Exposure
and
Animal
information:
1000
ppm
A%
SD
Mouse
8
$
17.8
1
19.36
2.02
Start
Time
r
4/
12/
01
IO:
04
AM
End
exposure
4/
12101
4104
PM
MS
Start
Time
MS
End
Time
Total
9.02
hr
GC
Conditions/
Comments
Flow
­
2.5
Llmin
Generator
Temperature
69
C
2
hr
into
exposure
animals
let­

3
hr
extremely
lethargic,
no
coordination.

4
hr
­
2
laying
on
side,
shallow,
labored
breathing
6
hr
­
all
animals
slow,
shallow,
labored
breathing,
completely
unconscious
MS/
MS
Conditions/
Comments
Flow
183mVmin
1
hr
post
exposure
­
animals
still
unconscious
2­
3
hr
post
exposure
­
animals
beginning
to
become
active,
still
lethargic
and
uncoordinated
4.5
hr
post
eposure
­
1
animal
dead,
1
still
unconscious
6
hr
post
exposure
­
all
living
animals
lethargic
and
uncoordinated
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Table
D­
3.
Exposure
and
Animaf
information:
500
ppm
+$$/
I
s,,
r&/

AQ
20.48
SD
I
.44
Page
57
of
69
Start
Time
4116/
01
9:
20
AM
End
exposure
4116101
I:
20
PM
Time
between
Exposureend
and
MS
Start
MS
Start
Time
0:
03:
45
min
MS
End
Time
MS
run
hr
GC
Conditions/
Comments
Flow
­
2.5
Llmin
IO:
40
am
animals
OK
1120
am
­
animals
lethargic
I:
00
pm
­
3
lethargic,
2
anesthetized
MS/
MS
Conditions/
Comments
Flow
181
mHmin
All
animals
awake,
2
very
slightly
uncoordinated
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Table
D­
4.
Exposure
and
Animal
information:
Page
58
of
69
250
ppm
Avg
19.62
SD
1.09
Start
Time
1
4/
18/
01
9:
07
AM
1
End
exposure
3:
07
PM
MS
Start
Time
MS
End
Time
MS
run
Time
between
Exposure
end
and
MS
Start
02:
30
min
hr
GC
Conditions/
Comments
Flow
­
2.5
L/
min
MS/
MS
Conditions/
Comments
Flow
179
mllmin
1,1,23richlorethane:
PK
in
rats
and
mice
Page
59
of
69
Project
No.
41608
Table
D­
5.
Off­
Gassing:
6
hr
inhalation
to
1000
ppm
1,1,2­
TCE
Time
Post
Exposure
(hr)
0.05
0.07
0.08
0.09
0.11
0.12
0.13
0.15
0.16
0.17
0.18
0.20
0.21
0.22
0.24
0.25
0.25
0.26
0.27
0.27
0.28
0.28
0.29
0.31
0.33
0.36
0.38
0.41
0.43
0.46
0.48
0.51
0.54
0.56
0.59
0.61
0.64
0.66
0.69
Chamber
Cont.
`P$­
yl
40:
1
30.3
31.6
34.7
34.9
33.3
32.2
32.1
31.6
32.0
31.5
32.9
32.9
31.9
30.3
31.1
30.0
32.5
33.4
33.7
33.5
34.2
32.3
30.9
31.5
34,4
37.1
38.1
37.9
38.7
37.1
41.1
45.5
43.4
41.5
40.4
37.8
38.8
Time
Post
Exposure
(hr)
0.71
0.74
0.75
0.77
0.78
0.80
0.82
0.84
0.85
0.87
0.88
0.90
0.92
0.93
0.94
0.95
0.97
0.99
1.02
1.05
1.08
1.12
1.15
1.18
1.22
1.25
1.28
1.31
1.35
1.38
1.41
1.44
1.48
1.51
1.54
1.58
1.61
1.64
1.67
Chamber
Cont.

`E
42:
7
40.9
43.2
45.0
48.4
49.4
49.6
45.4
47.6
43.4
41.5
41.4
43.1
43.1
42.9
42.0
39.6
37.8
37.5
39.3
40.8
40.5
42.6
43.9
39.1
39.3
37.5
38.5
39.7
37.2
35.5
37.4
36.7
36.1
38.9
42.3
41.7
42.5
Time
Post
Exposure
(hr)
1.71
1.74
1.77
1.80
1.84
1.87
1.90
1.93
1.97
2.01
2.05
2.10
2.15
2.20
2.24
2.29
2.34
2.39
2.43
2.48
2.53
2.58
2.62
2.67
2.72
2.76
2.81
2.86
2.91
2.95
3.00
3.05
3.10
3.14
3.19
3.24
3.29
3.33
3.38
Chamber
Cont.
`~
W?

47.3
45.5
43.8
45.1
45.6
43.2
42.8
43.0
43.8
47.4
44.8
40.3
41.9
45.4
39.2
40.6
42.1
44.0
51.5
54.7
53.6
57.0
56.4
58.2
60.9
65.9
73.9
63.3
59.1
61.7
60.4
59.4
58,3
:
57.8
58.6
60.7
70.3
72.5
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Time
Post
Exposure
(hr)
3.43
3.47
3.52
3.56
3.60
3.64
3.69
3.73
3.77
3.81
3.85
3.90
3.94
3.98
4.02
4.06
4.11
4.15
4.19
4.23
4.27
4.32
4.36
4.40
4.44
4.48
4.53
4.57
4.61
4.65
4.69
4.74
4.78
4.82
4.86
4.90
4.95
4.99
5.03
5.07
Chamber
Cont.
';
gy
'

68:
4
70.5
75.6
73.4
72.9
75.1
69.9
70.2
63.8
65.3
68.6
71.0
71.4
69.5
67.9
66.0
60.5
59.6
64.1
60.2
59.4
63.0
66.1
63.1
66.5
65,9
57.5
60.4
64.3
59.9
60.9
59.8.
56.1
53.9
49.7
49.2
49.4
47.6
46.8
Time
Post
Exposure(
hr)
5.11
5.16
5.20
5.24
5.28
5.32
5.37
5.41
5.45
5.49
5.53
5.58
5.62
5.66
5.70
5.74
5.79
5.83
5.87
5.91
5.95
6.00
6.04
6.08
6.12
6.16
6.21
6.25
6.29
6.33
6.37
6.42
6.46
6.50
6.54
6.58
6.63
6.67
6.71
6.75
Chamber
Cont.

'4psp"
7'

51:
o
49.9
50.4
45.2
48.3
47.2
44,3
43.5
41.2
38.3
42.9
43.3
43.2
41.0
39.6
39.9
39.1
38.1
40.0
39.4
38.5
38.0
36.0
35.3
35.3
34.4
33.6
32,4
32.2
33.8
33.5
30.1
32.6
29.9
30.2
31.2
32,2
29.1
28.0
Page
60
of
69
Chamber
Time
Post
Cont.
Exposure(
hr)
@pm>
6.79
~26.6
6.84
25.4
6.88
25.6
6.92
26.3
6.96
24.9
7.00
26.2
7.05
26.4
7.09
24.6
7.13
25.2
7.17
25.1
7.21
23.6
7.26
23.4
7.30
22.5
7.34
21.8
7.38
20.4
7.42
21.0
7.47
21.4
7.51
21.2
7.55
20.9
7.59
20.0
7.63
18.6
7.68
17.7
7.72
17.0
7.76
16.3
7.80
15.3
7.84
15.1
7.89
14.4
7.93
13,8
7.97
13.3
8.01
12.9
8.05
14.6
8.12
16.8
8.18
15.3
8.24
14.5
8.31
12.7
8.37
10.9
.
.
8.44
9.4
8.50
8.2
8.57
7.6
8863
8.1
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Page
61
of
69
Project
No.
41608
Chamber
Chamber
Chamber
Time
Post
Cont.
Time
Post
Cont.
Time
Post
Cont.
Exposure
(hr)
Exposure
(hr)
Exposure
(hr)
8.70
8.83
8.96
8.76
8.89
9.02
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Page
62
of
69
Project
No.
41608
Table
D­
6.
Off­
Gassing:
4
hr
inhalation
to
500
ppm
1,1,2­
TCE
Time
Post
Exposure(
hr)
0.09
0.10
0.12
0.14
0.15
0.17
0.19
0.20
0.22
0.24
0.25
0.27
0.29
0.30
0.32
0.34
0.35
0.37
0.39
0.40
0.42
0.44
0.45
0.47
0.49
0.50
0.52
0.54
0.55
0.57
0.59
0.60
0.62
0.64
0.65
0.67
0.69
0.70
0.72
Chamber
Cont.

i"
pY:
15:
24
16.78
17.63
17.14
15.11
13.86
13.37
13.03
12.97
12.57
12.23
11.99
11.82
12.03
11.89
11.57
11.61
11.46
11.30
11.00
10.61
10.40
10.36
10.66
10.34
9.88
9,85
9.85
9.48
8.97
9.08
8.90
8.25
8.08
8.39
8.81
8.38
7.56
Time
Post
Exposure(
hr)
0.74
0.75
0.77
0.79
0.80
0.82
0.84
0.85
0.87
0.89
0.90
0.92
0.94
0.95
0.97
0.99
1.00
1.02
1.04
1.05
1.07
1.09
1.10
1.12
1.14
1.15
1.17
1.19
1.20
1.22
1.24
1.25
1.27
1.29
1.30
1.32
1.34
1.35
1.37
Chamber
Cont.
';?
g
7:
49
7.16
6.84
7.27
7.83
7.83
7.55
6.92
6.82
7.31
7.15
6.91
7.06
7.35
7.02
6.31
6.10
6.38
6.38
5.89
6.10
6.69
6.62
6.45
6.71
7.24
7.29
7.37
7.77
7.47
6.69
6.57
6.07
5.28
5.16
5.07
4.95
5.33
Time
Post
Exposure(
hr)
1.39
1.40
1.42
1.44
1.45
1.47
1.49
1.50
1.52
1.54
1.55
1.57
1.59
1.60
1.62
1.64
1.65
1.67
1.69
1.70
1.72
1.74
1.75
1.77
1.79
1.80
1.82
1.84
1.85
1.87
1.89
1.90
1.92
1.94
1,95
1.97
1.99
2.00
2.02
Chamber
Cont.

(~
ps"
z'

5:
99
6.03
5.93
5.52
4.93
4.52
4.12
4.10
4.28
4.37
4.64
4.93
5.08
4.94
4.83
5.01
5.02
4.49
4.10
3.99
3.93
3.86
3.97
4.05
3.81
3.46
3.20
3.24
3.35
3.41
3.73
4.01
4.04
4.09
­.
4.37
4.51
4.57
4.54
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Page
63
of
69
Project
No.
41608
Chamber
Time
Post
Cont.
Exposure
(hr)
0w­
Q
2.04
2.05
2.07
2.09
2.10
2.12
2.14
2.15
`2.17
2.19
2.20
2.22
2.24
2.25
2.27
2.29
2.30
2.32
2.34
2.35
2.37
2.39
2.40
2.42
2.44
2.45
2.47
2.49
2.50
2.52
2.54
2.55
2.57
2.59
2.60
2.62
2.64
2.65
2.67
2.69
4.27
3.95
3.82
3.71
3.48
3.50
3.70
3.65
3.51
3.62
3.55
3.25
3.17
3,12
3.10
3.04
3.11
3.07
2.87
2.81
2.84
2.95
2.91
2.87
2.85
2.75
2.74
2.78
2.56
2.44
2,61
2.86
2.89
2.79
2.75
2.63
2.45
2.22
1.92
2.03
Time
Post
Exposure
(hr)
2.71
2.72
2.74
2.76
2.77
2.79
2.81
2.82
2.84
2.86
2.87
2.89
2.91
2.92
2.94
2.96
2.97
2.99
3.01
3.02
3.04
3.06
3.07
3.09
3.11
3.12
3.14
3.16
3.17
3.19
3.21
3.22
3.24
3.26
3.27
3.29
3.31
3.32
3.34
3.36
Chamber
Cont.

`2pplrl'

2:
04
2.09
2.09
2.08
2.15
2.12
1.92
1.75
1.74
1.66
1.65
1.75
1.92
1.96
1.88
1.76
1.68
1.59
1.65
1.75
1.73
1.77
1.68
1.64
1.67
1.56
1.36
1.35
1.37
1.25
1.18
1.19
1.22
1.22
1.32
1.30
1.21
1.22
1.22
Chamber
Time
Post
Cont.
Exposure
(hr)
(w­
N
3.37
1.22
3.39
1.16
3.41
1.18
3.42
1.21
3.44
1.18
3.46
1.11
3.47
1.16
3.49
1.23
3.51
1.17
3.52
1.16
3.54
1.16
3.56
1.09
3,57
1.06
3.59
1.00
3.61
0.96
3.62
0.91
3.64
0.87
3.66
0.94
3.67
1.03
3.69
1.00
3.71
0.96
3.72
0.91
3.74
0.84
3.76
0.75
3.77
0.69
3.79
0.68
3.81
0.71
3.82
0.74
3.84
0.72
3.86
0.74
3.87
0.76
3.89
0.77
3.91
0.76
3.92
0.73
3.94
0.73
3.96
0.74
..
3.97
0.76
3.99
0.71
4.01
0.72
4.02
0.76
1,1,2­
trichlorethane:
PK
in
rats
and
mice
iroject
No.
41608
Chamber
Time
Post
Cont.
Exposure(
hr)
4.04
';;;
I
4.06
0:
61
4.07
0.59
4.09
0.61
4.11
0.59
4.12
0.59
4.14
0.59
4.16
0.57
4.17
0.59
4.19
0.60
4.21
0.59
4.22
0.53
4.24
0.53
4.26
0.56
4.27
0.54
4.29
0.52
4.31
0.52
4.32
0.54
4.34
0.52
4.36
0.48
Time
Post
Exposure(
hr)
4.37
4.39
4.41
4.42
4.44
4.46
4.47
4.49
4.51
4.52
4,54
4.56
4.57
4.59
4.61
4.62
4.64
4.66
4.67
4.69
Chamber
Cont.

`opP$
'

0:
49
0.49
0.54
0.54
0.52
0.50
0.50
0.48
0.41
0.38
0.42
0.44
0.44
0.40
0.37
0.39
0.39
0.37
0.37
Page
64
of
69
Time
Post
Exposure(
hr)
4.71
4.72
4.74
4.76
4.77
4.79
4.81
4.82
4.84
4.86
4.87
4.89
4.91
4.92
4.94
4.96
4.97
4.99
5.01
Chamber
Cont.
.
';
P$

0:
43
0.41
0.43
0.46
0.45
0.42
0.46
0.47
0.41
0.40
0.42
0.40
0.34
0.31
0.31
0.30
0.30
0.23
1,1,2­
trkhlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
65
of
69
Table
D­
7.
Off­
Gassing:
5
hrinhalationto
250
ppm
1,1,2­
TCE
Time
Post
Exposure(
hr)
0.06
0.08
0.10
0.11
0.13
0.15
0.16
0.18
0.20
0.21
0.23
0.25
0.26
0.28
0.30
0.31
0.33
0.35
0.36
0.38
0.40
0.41
0.43
0.45
0.47
0.48
0.50
0.52
0.53
0.55
0.57
0.58
0.60
0.62
0.63
0.65
0.67
0.68
0.70
Chamber
Cont.

!%
5:
710
5.905
6.067
5.468
4.361
3.835
3.546
3.430
3.292
3.177
3.114
3.042
2.904
2.755
2.653
2.526
2.415
2.396
2.358
2.311
2,262
2.218
2.174
2.055
1.901
1.828
1.788
1.729
1.654
1.627
1.555
1.463
1.415
1.410
1.358
1.326
1.430
1.446
Time
Post
Exposure(
hr)
0.72
0.73
0.75
0.77
0.78
0.80
0.82
0.83
0.85
0.87
0.88
0.90
0.92
0.93
0.95
0.97
0.98
1.00
1.02
1.03
1.05
1.07
1.08
1.10
1.12
1.13
1.15
1.17
1.18
1.20
1.22
1.23
1.25
1.27
1.28
1.30
1.32
1.33
1.35
Chamber
Cont.

!!%­:
1:
321
1.272
1.258
1.119
1.030
1.017
1.014
1.095
1.102
1.043
0.996
0,924
0,945
0.954
0.957
0.896
0.808
0.822
0.801
0.736
0.718
0.754
0.755
0.740
0.749
0.726
0.662
0.675
0.692
0.613
0.622
0.661
0.700
0.657
0.598
0.553
0.544
0.520
Chamber
Time
Post
Cont.
Exposure(
hr)
(PPm)
1.37
0.499
1.38
0,508
1.40
0.537
1.42
0.521
1.43
0.486
1.45
0.477
1.47
0.459
1.48
0.449
1.50
0.457
1.52
0.449
1.53
0.455
1.55
0.452
1.57
0.446
1.58
0.444
1.60
0.473
1.62
0.470
1.63
0.429
1.65
0.401
1.67
0.388
1.68
0,352
1.70
0.360
1.72
0.375
1.73
0.363
1.75
0.329
1.77
0.339
1.78
0.362
1.80
0.331
1.82
0.343
1.83
0.340
1.85
0.328
1.87
0.323
1.88
0.331
1,90
0.304
1.92
0.295
.
.
1.93
0.313
1.95
0.262
1.97
0.259
1.98
0.305
2.00
0.295
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
66
of
69
Time
Post
Exposure
(hr)
2.02
2.03
2.05
2.07
2.08
2.10
2.12
2.13
2.15
2.17
2.18
2.20
2.22
2.23
2.25
2.27
2.28
2.30
2.32
2.33
2.35
2.37
2.38
2.40
2.42
2.43
2.45
2.47
2.48
2.50
2.52
2.53
2.55
2.57
2.58
2.60
2.62
2.63
2.65
2.67
Chamber
Cone,

EL2
0:
240
0.295
0.290
0.230
0.223
0.268
0.267
0.228
0.225
0.252
0.276
0.255
0.235
0.242
0.235
0.247
0.285
0.263
0.253
0.252
0.230
0.222
0.217
0.252
0.255
0.242
0.256
0.248
0,216
0,209
0.242
0.237
0.212
0.213
0.214
0.233
0,251
0.238
0.205
Time
Post
Exposure
(hr)
2.69
2.70
2.72
2.74
2.75
2.77
2.79
2.80
2.82
2.84
2.85
2.87
2.89
2.90
2.92
2.94
2.95
2.97
2.99
3.00
3.02
3.04
3.05
3.07
3.09
3.10
3.12
3.14
3.15
3.17
3.19
3.20
3.22
3.24
3.25
3.27
3.29
3.30
3.32
3.34
Chamber
Cont.

E2
0:
236
0.209
0.192
0.208
0.221
0.228
0.192
0.184
0.203
0.189
0.172
0.181
0.166
0.159
0.152
0.186
0.193
0.173
0.198
0.195
0.177
0.168
0.148
0.139
0.153
0.176
0.187
0.177
0.179
0.196
0.237
0.213
0.162
0.166
0.170
0.146
0.140
0.117
0.093
Time
Post
Exposure
(hr)
3,35
3.37
3.39
3.40
3.42
3.44
3.45
3.47
3.49
3.50
3.52
3.54
3.55
3.57
3.59
3.60
3.62
3.64
3.65
3.67
3.69
3.70
3.72
3.74
3.75
3.77
3,79
3.80
3.82
3.84
3.85
3.87
3.89
3.90
3.92
3.94
3.95
3.97
3.99
4.00
Chamber
Cont.

K.­
01126
0.126
0.116
0.119
0.140
0.175
0.163
0.135
0.120
0.090
0.113
0.189
0.201
0.183
0.192
0.185
0.130
0.104
0.112
0.124
0.117
0.137
0.143
0.141
0.145
0.149
0.143
0.130
0.137
0.128
0.139
0.165
0.164
0.156
0.127
.'
0.1?.
4
0.099
0.084
0.096
1,1,2­
trichlorethane:
PK
in
rats
and
mice
Page
67
of
69
Project
No.
41608
Chamber
Chamber
Time
Post
Cont.
Time
Post
Cone.
Exposure(
hr)
(PPm)
Exposure(
hr)
(iv­
r­
0
4.02
0,117
4.04
0.159
4.05
0.185
4.07
0.157
4.09
0.105
4.10
0.112
4.12
0.119
4.14
0.127
4.15
0.098
4.17
0.101
4.19
0.124
4.20
0.107
4.22
0.114
4.24
0.140
4.25
0.151
4.27
0.126
4.29
0.133
4.30
0.131
4.32
0.115
4.34
0.094
4.35
0.115
4.37
0.123
4.39
0.138
4.40
0.088
4,42
0.028
4.44
0.067
4.45
0.093
4.47
0.087
4.49
0.053
4.50
0.095
4.52
0.108
4.54
0.092
4.55
0.071
4.57
0.083
4.59
0.096
4.60
0.110
4.62
0.097
4.64
0.077
4.65
0.088
4.67
0.055
4.69
0.031
4.70
0.043
4.72
0.065
4.74
0.072
4.75
0.082
4,77
0.119
4.79
0.096
4.80
0.077
4.82
0.080
4.84
0.070
4.86
0.050
4.87
0.057
4.89
0.@
72
4.91
0.084
4.92
0.083
4.94
0.052
4.96
0.057
1,1,23richlorethane:
PK
in
rats
and
mice
Project
No.
41608
Page
68
of
69
Appendix
E:
Partition
Coefficients
Table
E­
l.
Time
and
volume
determination
using
rat
blood.
Incubation
Sample
Volume
Time
(hr)
(9)
Partition
Coefficient
Averaqe
1
0.500
58.0
1
0.498
46.1
52.0
1
1.020
57.5
1
1.036
50.1
53.8
1
2.030
47.8
1
2.010
53.9
1
2.010
53.9
51.9
rfI
3.53
3
0.500
54.6
3
0.500
50.3
3
0.497
50.2
51.7
f:
2.52
3
1.02
54.1
3
1.036
61.7
3
1,030
55.1
56.9
­t
4.17
Page
69
of
69
Table
E­
2.
Blood:
Air
Partition
Coefficient:
Female
B6C3Fl
Mouse
Blood
Sample
Volume
(g)
Partition
Coefficient
Average
1
0.509
82.8
2
0.502
63.1
3
0.509
73.0
4
0.501
76.0
5
0.498
61.5
6
0.502
69.9
71.0
Ik
8.03
Pool
A
1.017
65.1
;::
i
:
1.026
1.009
74.3
66.9
68.7
k
4.87
Note:
The
blood
from
4
extra
animals
was
pooled
with
left­
over
blood
from
the
original
6
animals,
and
partition
coefficients
determined
in
1
ml
volumes
of
the
pooled
blood.