Document ID: EPA-HQ-OPPT-2003-0067-0004
Agency: epa
Document Type: Supporting & Related Material
Title: 
Posted Date: 2003-11-17T05:00Z

83
Ammonia
(
CAS
No.
7664­
41­
7)
Draft,
Unpublished
Version.
Government
of
Canada,
Environment
Canada.:

Arillo,
A.,
Margiocco,
C.,
Melodia,
F.,
Mensi,
P.,
and
Schenone,
G.
1981.
Ammonia
toxicity
mechanism
in
fish:
studies
on
rainbow
trout
(
Salmo
gairdneri
Rich.).
Ecotoxicol.
Environ.
Safety
5:
316­
328.
Kloppic,
E.,
Jasobasch,
G.,
and
Rapoprt,
S.
1967.
Enhancement
of
the
glycolytic
rate
by
action
of
ammonium
ion
on
phosphofructokinase
activity.
Original
in
German.
Acta.
Biol.
Med.
18:
37­
42.
Fromm,
P.
O.,
and
Gillette,
J.
R.
1968.
Effect
of
ambient
ammonia
on
blood
ammonia
and
nitrogen
excretion
of
rainbow
trout
(
Salmo
gaidneri).
Comp.
Biochem.
Physiol.
26:
887­
896.
Person­
LeRuyet,
J.,
Boeuf,
G.
Zambonin­
Infant,
J.,
Helgason,
S.,
and
Le
Roux,
A.
1998.
Short­
term
physiological
changes
in
turbot
and
seabream
juveniles
exposed
to
exogenous
ammonia.
Comp.
Biochem.
Physiol.
119A:
511­
518.
Randall,
D.
J.,
and
Wicks,
B.
J.
1999.
Fish:
ammonia
production,
excretion,
and
toxicity.
In
press.
Paper
presented
in
the
Fifth
International
Symposium
on
Fish
Physiology,
Toxicology,
and
Water
Quality.
University
of
Hong
Kong.

4.9
ADDITIONAL
REMARKS
(
a)
Remarks:
The
toxicity
of
ammonia
to
aquatic
organisms
is
highly
dependent
on
physicochemical
factors,
most
notably
pH
because
of
its
importance
in
chemical
speciation.
The
acute
toxicity
of
ammonia
is
also
influenced
to
a
lesser
degree
by
temperature,
carbon
dioxide,
dissolved
oxygen,
and
salinity.
In
aqueous
solution,
ammonia
exists
primarily
in
two
forms,
un­
ionized
ammonia
(
NH3)
and
ammonium
ion
(
NH4
+),
which
are
in
equilibrium
with
each
other
according
to
the
following
established
relationship:

NH3(
aq)
+
H2O
 
NH4
+
+
OH­

In
general,
as
pH
increases,
the
fraction
of
the
total
ammonia
which
is
un­
ionized
increases.
It
is
this
un­
ionized
ammonia
(
NH3)
which
is
generally
considered
to
be
the
primary
cause
of
toxicity
in
aquatic
systems.
Un­
ionized
ammonia
(
NH3)
is
more
toxic
to
aquatic
organisms
than
the
ammonium
ion
(
NH4
+)
because
the
un­
ionized
form
is
readily
soluble
in
the
lipid
of
the
cell
membrane
and
is
rapidly
absorbed
by
the
gill.
In
contrast,
the
charged
ion
is
not
readily
passed
through
the
charged­
line
hydrophobic
space
in
the
membrane.
EPA
studies
indicate
that
un­
ionized
ammonia
is
190
times
more
toxic
to
guppies
than
ammonium.
84
Ammonia
(
CAS
No.
7664­
41­
7)
Multiple
EPA
studies
have
shown
that
over
the
pH
range
of
6.5­
9.0,
the
toxicity
of
un­
ionized
ammonia
(
NH3)
increases
as
the
pH
decreases.
This
is
either
because
the
additional
hydrogen
ion
(
H+)
concentration
at
lower
pHs
increases
the
toxicity
of
unionized
ammonia
or
because
the
ammonium
ion
(
NH4
+)
exerts
some
level
of
toxicity
at
lower
pH.
However,
it
would
be
an
oversimplification
to
attribute
the
toxic
action
to
only
the
ammonium
ion
at
low
pH
and
to
only
unionized
ammonium
at
high
pH
because
most
likely
both
forms
participate
when
total
ammonia
concentration
is
high
enough
to
cause
toxicity
symptoms.
A
joint
toxicity
model
has
been
proposed,
with
ammonium
causing
most
toxicity
at
high
pH
values
and
ammonium
ion
also
contributing
to
toxicity
at
lower
pH
values.
This
is
supported
through
studies
demonstrating
that
at
a
low
pH
a
new
inward
flux
of
ammonium
ion
can
occur
across
the
gills
of
aquatic
species.
Reference:
Clement
Associates,
Inc.
1990.
Health
Effects
Assessment
for
Ammonia.
Prepared
for
The
Fertilizer
Institute,
Washington,
D.
C.
Erickson,
R.
J.
1985.
An
evaluation
of
mathematical
models
for
the
effects
of
pH
and
temperature
on
ammonia
toxicity
to
aquatic
organisms.
Water
Res.
19:
1047­
1058.
Evans,
D.
E.
and
Cameron,
J.
N.
1986.
Gill
ammonia
transport.
J.
Exp.
Zool.
239:
17­
23.
Thurston,
R.
V.,
Russo,
R.
C.,
Phillips,
G.
R.
1983.
Acute
toxicity
of
ammonia
to
fathead
minnows.
Trans.
Am.
Fish.
Soc.
112:
705­
711.
U.
S.
Environmental
Protection
Agency
(
USEPA).
1998c.
1998
Update
of
Ambient
Water
Quality
Criteria
for
Ammonia.
Office
of
Water,
Washington,
D.
C.
EPA
822­
R­
98­
008.
U.
S.
Environmental
Protection
Agency
(
USEPA).
1985.
Ambient
Water
Quality
Criteria
for
Ammonia
 
1984.
Washington,
D.
C.:
Office
of
Water
Regulations
and
Standards,
Criteria
and
Standards
Division.
EPA­
504/
5­
85­
006.

(
b)
Remarks:
The
amount
of
un­
ionized
ammonia
as
a
percent
of
total
ammonia
in
aqueous
solution
is
dependent
on
pH
and
temperature.
The
authors
derive
this
relationship
and
provide
a
table
showing
the
percent
of
un­
ionized
ammonia
at
various
pH
values
and
temperatures.
Reference:
Emerson,
K.,
Russo,
R.
C.,
Lund,
R.
E.,
and
Thurston,
R.
V.
1975.
Aqueous
ammonia
equilibrium
calculations:
effect
of
pH
and
temperature.
J.
Fish.
Res.
Board
Can.
32:
2379­
2383.
85
Ammonia
(
CAS
No.
7664­
41­
7)
5.
TOXICITY
*
5.1
ACUTE
TOXICITY
5.1.1
ACUTE
ORAL
TOXICITY
Not
an
applicable
route
of
exposure.

5.1.2
ACUTE
INHALATION
TOXICITY
(
a)
Type:
LC0
[
];
LC100
[
];
LC50
[
X];
LCL0
[
];
Other
[
]
Species/
strain:
Rat
(
Wistar)
Exposure
Time:
60
minutes
Value:
LC50
(
male)
=
9,850
mg/
m3
air
(
14,111
ppm)
LC50
(
female)
=
13,770
mg/
m3
air
(
19,727
ppm)
Method:
Male
and
female
SPF­
bred
rats
were
exposed
to
an
ammonia
atmosphere.
Each
dose
group
consisted
of
5
males
and
5
females
and
they
were
exposed
to
an
ammonia/
air
mixture
at
a
rate
of
25
L/
min.
Animals
were
exposed
for
10,
20,
40,
or
60
minutes.
After
exposure,
they
were
housed
5
per
cage
for
an
observation
period
of
14
days.
Food
and
water
were
available
during
the
observation
period,
but
were
withheld
during
exposure.
Body
weight
was
recorded
at
days
1,
2,
4,
7,
and
14.
GLP:
Yes
[
]
No
[
]
?
[
X]
Test
substance:
Liquid
ammonia
(
NH3)
in
a
pressurized
cylinder
(
UKF­
SBB,
The
Netherlands)
Remarks:
Clinical
symptoms
included
eye­
irritation,
especially
in
animals
exposed
for
60
minutes,
wet
noses,
and
nasal
discharge.
Autopsy
revealed
haemorrhagic
lungs.
Reference:
Appelman,
L.
M.,
ten
Berge,
W.
F.,
and
Reuzel,
P.
G.
J.
1982.
Acute
inhalation
toxicity
study
of
ammonia
in
variable
exposure
periods.
Am.
Ind.
Hyg.
Assoc.
J.
43(
9):
662­
665.

(
b)
Type:
LC0
[
];
LC100
[
];
LC50
[
X];
LCL0
[
];
Other
[
]
Species/
strain:
Albino
ICR
mice
Exposure
Time:
1
hour
Value:
LC50
=
4230
ppm
un­
ionized
NH3
Method:
Groups
of
12
mice
each
were
exposed
simultaneously
to
three
concentrations
of
NH3
gas
(
1,190­
4,860
ppm
depending
on
which
of
the
three
experiments)
in
air
using
a
dynamic
inhalation
exposure
system.
The
control
group
was
exposed
to
air
only.
The
mice
were
allowed
free
access
to
food
and
water
during
the
experiment,
except
while
in
chamber
tubes.
The
mice
were
acclimated
for
at
least
1week
prior
to
the
experiment.
Sampling
86
Ammonia
(
CAS
No.
7664­
41­
7)
and
analyses
were
conducted
every
3­
5
minutes.
Animals
were
observed
for
14
days
following
exposure.
GLP:
Yes
[
]
No
[
]
?
[
X]
Test
substance:
Ammonia
(
NH3)
gas
(
Matheson,
East
Rutherford,
NJ)
Remarks:
At
high
NH3
concentrations,
mortality
usually
occurred
within
30
minutes
of
initiation
of
exposure.
Twenty­
five
percent
of
animals
exposed
to
3950
ppm
and
greater
did
not
survive.
Percent
mortality
in
higher
concentrations
(
4220­
4860
ppm)
ranged
from
41.6
to
100%.
The
lungs
of
animals
that
died
during
exposure
were
congested
with
evidence
of
hemorrhage.
The
lungs
of
animals
from
every
treatment
group
sacrificed
displayed
a
mild
to
moderate
degree
of
chronic
focal
pneumonitis
histologically.
The
liver
and
heart
weights
were
increased
in
animals
that
died
during
exposure
to
4860
ppm.
Reference:
Kapeghian,
J.
C.,
Mincer,
H.
H.,
Jones,
A.
B.,
Verlangieri,
A.
J,
and
Waters,
I.
W.
1982.
Acute
inhalation
toxicity
of
ammonia
in
mice.
Bull.
Environ.
Contam.
Toxicol.
29:
371­
378.

(
c)
Type:
LC0
[
];
LC100
[
];
LC50
[
X];
LCL0
[
];
Other
[
]
Species/
strain:
Rat
Exposure
time:
1
hour
Value:
LC50
=
5137
mg/
m3
(
7338
ppm)
Method:
Rats
were
exposed
for
one
hour
to
several
concentrations
of
ammonia.
GLP:
Yes
[
]
No
[
X]
?
[
]
Test
substance:
Ammonia
(
NH3)
gas
Remarks:
Inhalation
produced
immediate
nasal
and
eye
irritation
followed
by
labored
breathing
in
all
test
groups.
Surviving
rats
necropsied
after
14
days
showed
moderate
mottling
of
the
liver
at
the
5474
and
6888
mg/
m3
(
7820
and
9840
ppm)
test
levels.
Reference:
MacEwen,
J.
D.
and
Vernot,
E.
H.
1972.
Toxic
hazards
research
unit
annual
technical
report.
NTIS
AD
755­
358.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

(
d)
Type:
LC0
[
];
LC100
[
];
LC50
[
X];
LCL0
[
];
Other
[
X]
Species/
strain:
Rat
Exposure
time:
5,
15,
30
or
60
minutes
Value:
LC50
(
5­
m)
=
18,693
mg/
m3
(
26,704
ppm)
LC50
(
15­
m)
=
11,342
mg/
m3
(
17,372
ppm)
LC50
(
30­
m)
=
7035
mg/
m3
(
10,050
ppm)
LC50
(
60­
m)
=
7939
mg/
m3
(
11,342
ppm)
NOEL
(
5,
15,
30,
&
60­
m)
=
100
mg/
m3
(
144
ppm)
Method:
White
rats
were
exposed
to
high
concentrations
of
ammonia
(
6000,
3000,
and
1000
mg/
m3,
equivalent
to
6814,
4307
and
1436
ppm)
via
inhalation.
GLP:
Yes
[
]
No
[
X]
?
[
]
Test
substance:
Ammonia
(
NH3)
gas
87
Ammonia
(
CAS
No.
7664­
41­
7)
Remarks:
Other
effects
included
dyspnoea,
irritation
of
the
respiratory
tract
and
eyes,
cyanosis
of
the
extremities,
and
increased
excitability.
In
associated
studies,
rats
exposed
to
3000,
1000
or
300
mg/
m3
(
4307,
1436,
431
ppm)
exhibited
a
drop
in
static
muscular
tension
and
other
sublethal
effects.
No
effects
were
observed
in
rats
exposed
to
100
mg/
m3
(
144
ppm)
for
5,
15,
30
or
60
minutes.
Reference:
Propkopieva,
A.
S.
et
al.
1973.
Materials
for
a
toxicological
characteristic
of
the
long­
term
effect
of
ammonia
on
the
organisms
of
animals
after
brief
exposure.
Gig.
Tr.
Prof.
Zabol.
(
Russian)
6:
56­
57.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

(
e)
Type:
LC0
[
];
LC100
[
];
LC50
[
X];
LCL0
[
];
Other
[
]
Species/
strain:
Mouse
Exposure
time:
1
hour
Value:
LC50
=
3386
mg/
m3
(
4837
ppm)
Method:
Mice
were
exposed
for
one
hour
to
several
concentrations
of
ammonia.
GLP:
Yes
[
]
No
[
X]
?
[
]
Test
substance:
Ammonia
(
NH3)
gas
Remarks:
Inhalation
produced
immediate
nasal
and
eye
irritation
followed
by
labored
breathing
in
all
test
groups.
Mild
congestion
of
the
liver
was
also
observed
in
surviving
mice
in
the
two
highest
dose
levels
of
3185
and
4004
mg/
m3
(
4550
and
5720
ppm).
Reference:
MacEwen,
J.
D.
and
Vernot,
E.
H.
1972.
Toxic
hazards
research
unit
annual
technical
report.
NITS
AD
755­
358.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

(
f)
Type:
LC0
[
];
LC100
[
];
LC50
[
X];
LCL0
[
];
Other
[
]
Species/
strain:
Mouse
Exposure
time:
10
minutes
Value:
LC50
=
7060
mg/
m3
(
10,152
ppm)
Method:
Groups
of
20
mice
were
exposed
to
9
concentrations.
GLP:
Yes
[
]
No
[
X]
?
[
]
Test
substance:
Ammonia
(
NH3)
gas
Remarks:
The
LC50
was
calculated
from
the
mortality
observed
at
10
days.
Most
deaths
occurred
rapidly,
with
equally
rapid
recovery
of
survivors.
Reference:
Silver,
S.
D.
and
McGrath,
F.
P.
1948.
J.
Ind.
Hyg.
Toxicol.
30:
7­
9.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

(
g)
Type:
LC0
[
];
LC100
[
];
LC50
[
];
LCL0
[
];
Other
[
X]
88
Ammonia
(
CAS
No.
7664­
41­
7)
Species/
strain:
Rabbit,
cat
Exposure
time:
1
hour
Value:
See
remarks
Method:
Animals
were
placed
in
a
static
gassing
chamber
of
400
L
capacity
and
exposed
to
from
3.5
to
8.7
mg
un­
ionized
NH3/
L.
Experiments
were
performed
upon
54
rabbits
and
18
cats
subdivided
into
6
groups.
GLP:
Yes
[
]
No
[
X]
?
[
]
Test
substance:
Ammonia
(
NH3)
gas
Remarks:
Exposure
to
ammonia
increased
the
respiratory
tract
fluid
output
two­
to
three­
fold
in
cats
and
rabbits.
Inhalation
of
ammonia
had
no
appreciable
effect
upon
the
water
content
of
respiratory
tract
tissues
of
rabbits
and
cats.
Following
gassing
with
ammonia,
the
iron
content
of
the
tracheal,
bronchial,
and
alveolar
portions
increased
in
rabbits.
There
was
an
initial
slight
drop
in
blood
hemoglobin
in
the
first
4
or
5
hours
of
exposure,
followed
by
a
return
to
normal
levels
by
8
hours
in
both
cats
and
rabbits.
Definite
lipemia
was
observed
in
rabbits.
Reference:
Boyd,
E.
M.,
MacLachlan,
M.
L.,
and
Perry,
W.
F.
1944.
Experimental
ammonia
gas
poisoning
in
rabbits
and
cats.
J.
Ind.
Hyg.
Toxicol.
26:
29­
34.

(
h)
Type:
LC0
[
];
LC100
[
];
LC50
[
];
LCL0
[
];
Other
[
X]
(
RD50)
Species/
strain:
Male
Swiss­
Webster
mice
Exposure
time:
30
minutes
Value:
A
concentration
of
212
mg/
m3
(
303
ppm)
decreases
the
respiratory
frequency
by
50%
(
RD50)
in
mice.
Method:
Mice
were
exposed
to
atmospheric
concentrations
ranging
from
70­
560
mg/
m3
(
100­
1000
ppm).
GLP:
Yes
[
]
No
[
X]
?
[
]
Test
substance:
Ammonia
(
NH3)
gas
Remarks:
Ammonia
is
a
sensory
irritant
to
rodents.
Reference:
Barrow,
C.
S.,
Alarie,
Y.
and
Stock,
M.
F.
1978.
Sensory
irritation
and
incapacitation
evoked
by
thermal
decomposition
products
of
polymers
and
comparisons
with
known
sensory
irritants.
Arch.
Environm.
Health
33:
79­
88.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

(
i)
Type:
LC0
[
];
LC100
[
];
LC50
[
X];
LCL0
[
];
Other
[
]
Species/
strain:
Wistar
rats
(
male
and
female)
Exposure
time:
10,
20,
40,
or
60
minutes
Value:
LC50
(
10­
m)
=
28,130
mg/
m3
(
40,300
ppm)
LC50
(
20­
m)
=
19,960
mg/
m3
(
28,595
ppm)
LC50
(
40­
m)
=
14,170
mg/
m3
(
20,300
ppm)
LC50
(
60­
m)
=
11,590
mg/
m3
(
16,600
ppm)
Method:
Ten
animals
(
5
males,
5
females)
were
exposed
to
each
of
a
series
of
ammonia
concentrations
in
an
inhalation
cylinder
for
89
Ammonia
(
CAS
No.
7664­
41­
7)
10,
20,
40
or
60
minutes.
After
exposure,
rats
were
housed
in
wire
mesh
cages
for
an
observation
period
of
14
days.
GLP:
Yes
[
]
No
[
X]
?
[
]
Test
substance:
Ammonia
(
NH3)
gas
evolved
from
a
pressurized
cylinder
of
liquid
ammonia.
Remarks:
Clinical
signs
of
restlessness,
eye
irritation,
nasal
discharge,
mouth
breathing,
and
labored
respiration
were
seen
during
exposure.
Reference:
Appelman,
L.
M.,
ten
Berg,
W.
F.,
and
Reuzel,
P.
G.
J.
1982.
Acute
inhalation
toxicity
study
of
ammonia
in
rats
with
variable
exposure
periods.
Am.
Ind.
Hyg.
Assoc.
J.
43(
9):
662­
665.

(
j)
Type:
LC0
[
];
LC100
[
];
LC50
[
];
LCL0
[
];
Other
[
]
Species/
strain:
ICR
mice
(
male)
Exposure
time:
1
hour
Value:
LC50
=
2960
mg/
m3
(
4,230
ppm)
Method:
Four
groups
of
12
mice
were
exposed
to
each
of
three
concentrations
of
NH3
gas
in
air.
The
1
hour
exposure
was
followed
by
a
14
day
observation
period.
GLP:
Yes
[
]
No
[
X]
?
[
]
Test
substance:
Ammonia
(
NH3)
gas
Remarks:
Lungs
of
mice
that
died
during
exposure
were
diffusely
haemorrhagic.
A
mild
to
moderate
degree
of
chronic
focal
pneumonitis
was
also
observed.
Reference:
Kapeghian,
J.
C.,
Mincer,
H.
H.,
Jones,
A.
B.,
Verlangieri,
A.
J.,
and
Waters,
I.
W.
1982.
Acute
inhalation
toxicity
of
ammonia
in
mice.
Bull
Environ.
Contam.
Toxicol.
29:
371­
378.

(
k)
Type:
LC0
[
];
LC100
[
];
LC50
[
];
LCL0
[
];
Other
[
X]
Species/
strain:
Cat
Exposure
time:
10
minutes
Method:
Twenty
cats
were
randomly
placed
into
four
groups
of
five
cats
each.
Baseline
pulmonary
function
tests
were
performed.
All
the
animals
were
exposed
to
1,000
ppm
ammonia
gas
for
10
min.
One
group
was
euthanized
after
24
hours
and
the
other
three
groups
were
tested
on
days
7,
21,
and
35
post­
exposure.
GLP:
Yes
[
]
No
[
]
?
[
X]
Test
substance:
Anhydrous
ammonia
(
NH3)
Remarks:
Twenty­
four
hours
after
exposure,
cats
had
severe
dyspnea,
anorexia,
and
dehydration.
Auscultation
revealed
bronchial
breath
sounds,
sonorous
and
sibulent
ronchi,
and
course
rales.
Lungs
showed
varying
degrees
of
congestion,
hemorrhage,
edema,
interstitial
emphysema,
and
collapse.
There
was
no
significant
change
in
Anatomical
Dead
Space
(
ADS).
The
only
significant
change
in
Functional
Residual
Capacity
(
RFC)
was
on
day
21,
at
which
time
this
value
almost
doubled.
Seven
days
after
exposure
to
ammonia
gas,
the
epithelial
damage
was
healed.
90
Ammonia
(
CAS
No.
7664­
41­
7)
Reference:
Dodd,
K.
T.
and
Gross.
D.
R.
1980.
Ammonia
inhalation
toxicity
in
cats:
a
study
of
acute
and
chronic
respiratory
dysfunction.
Arch.
Environ.
Health
35(
1):
6­
14.

(
l)
Type:
LC0
[
];
LC100
[
];
LC50
[
];
LCL0
[
];
Other
[
X]
Species/
strain:
New
Zealand
White
rabbit
Exposure
time:
2.5
to
3.0­
hours
Value:
BUN
(
pre­
exposure)
=
19.4
mg/
100
mL
BUN
(
post­
exposure)
=
24.6
mg/
100
mL
Blood
pH
(
pre­
exposure)
=
7.34
Blood
pH
(
post­
exposure)
=
7.36
Blood
CO2
(
pre­
exposure)
=
14.3
meq/
L
plasma
Blood
CO2
(
post­
exposure)
=
18.9
meq/
L
plasma
Method:
Sixteen
adult
female
rabbits
were
exposed
to
50
ppm
in
experiment
1
and
100
ppm
ammonia
in
experiment
2.
Each
experiment
ran
from
7.5
to
9
hours
duration
with
ammonia
exposure
occurring
only
during
the
second
2.5
to
3.0
hour
segment.
GLP:
Yes
[
]
No
[
]
?
[
X]
Test
substance:
Anhydrous
ammonia
(
NH3)
Remarks:
The
decline
in
respiration
rate
was
essentially
linear
over
the
7.5
hour
period
for
both
groups
under
control
conditions.
The
decrease
in
respiration
rate
upon
exposure
to
ammonia
was
highly
significant
and
essentially
the
same
for
the
50
ppm
and
100
ppm
exposure
levels.
BUN
=
Blood
Urea
Nitrogen.
Reference:
Mayan,
M.
H.
and
Merilan,
C.
P.
1972.
Effects
of
ammonia
inhalation
on
respiration
rate
of
rabbits.
J.
Anim.
Sci.
34(
3):
447­
452.

(
m)
Type:
LC0
[
];
LC100
[
];
LC50
[
];
LCL0
[
];
Other
[
X]
Species/
strain:
Guinea
pigs
Exposure
time:
6
to
18­
weeks
Method:
Twelve
male
guinea
pigs
were
used
for
experimentation
and
6
for
controls.
Exposure
to
ammonia
was
for
6
hours
per
day,
5
days
per
week.
At
intervals
of
6
weeks,
4
experimental
and
2
control
animals
were
sacrificed.
GLP:
Yes
[
]
No
[
]
?
[
X]
Test
substance:
Anhydrous
ammonia
(
NH3)
Remarks:
Animals
exposed
for
6
and
12
weeks
showed
no
abnormalities
which
could
be
attributed
to
ammonia.
After
18
weeks,
animals
showed
considerable
congestion
of
spleens,
livers
and
kidneys,
and
early
degenerative
changes
in
the
suprarenal
gland.
Upper
tubules
of
the
kidneys
showed
fairly
marked
cloudy
swelling
with
precipitated
albumen
in
the
lumens
and
some
casts.
Reference:
Weatherby,
J.
H.
1952.
Chronic
toxicity
of
ammonia
fumes
by
inhalation.
Proc.
Soc.
Exp.
Biol.
Med.
81:
300­
301.

(
n)
Type:
LC0
[
];
LC100
[
];
LC50
[
];
LCL0
[
];
Other
[
X]
91
Ammonia
(
CAS
No.
7664­
41­
7)
Method:
Summary
of
animal
inhalation
studies
for
ammonia.
Remarks:
The
acute
effects
of
ammonia
in
animals
was
reviewed
as
part
of
a
health
effects
assessment
prepared
in
1990
for
The
Fertilizer
Institute.
In
summary,
acute
exposure
to
high
concentrations
of
ammonia
in
air
to
animals
can
result
in
death
[
LC50
values
as
low
as
2961
mg/
m3
(
3796
ppm)
in
mice
and
5137
mg/
m3
(
6586
ppm)
in
rats
for
a
one­
hour
exposure.]
Acute
exposures
to
ammonia
in
the
range
of
200­
700
mg/
m3
(
256­
897
ppm)
resulted
in
excessive
irritation
and
nasal
and
mouth
secretions,
depressed
respiratory
rates,
and
lung
edema
and
hemorrhage
[
it
should
be
noted
that
some
experiments
showed
no
ill
effects
at
levels
as
high
as
700
mg/
m3
(
897
ppm)].
One
frequently
observed
result
of
acute
exposure
is
the
cessation
of
tracheal
ciliary
activity.
For
directly
exposed
tracheas,
ciliary
beating
stopped
after
exposure
to
as
low
as
2.1
mg/
m3
(
3
ppm).
However,
experiments
which
produced
cessation
without
recovery
utilized
concentrations
in
the
range
of
280­
1400
mg/
m3
(
359­
1795
ppm).
Reference:
Clement
Associates,
Inc.
1990.
Health
Effects
Assessment
for
Ammonia.
Prepared
for
The
Fertilizer
Institute,
Washington,
D.
C.

(
o)
Type:
LC0
[
];
LC100
[
];
LC50
[
];
LCL0
[
];
Other
[
X]
Species/
strain:
Duroc
pigs
Exposure
time:
5­
weeks
Values:
NOAEL
=
61
ppm.
Methods:
Duroc
pigs
were
exposed
for
5
weeks
to
the
following
ammonia
concentrations:
8,
43,
72
and
102
mg/
m3
(
12,
61,
103,
and
146
ppm).
Exposures
were
conducted
under
controlled
environmental
conditions
of
21.1oC
dry­
bulb
temperature
and
77%
relative
humidity.
Thirty
six
pigs
with
an
average
initial
weight
of
approximately
54
kg
were
allotted
into
four
2.7
x
4.9
m.
compartments.
Ammonia
was
introduced
into
three
of
the
four
compartments
in
the
supply
air
ducts.
To
control
introduction,
a
corrosion
resistant
pressure­
regulating
valve
was
mounted
on
the
bulk
tank.
The
ammonia
was
metered
from
this
valve
into
a
low­
pressure
chamber
that
was
maintained
at
0.14
to
0.21
kg
per
square
centimeter
gauge
pressure.
Three
stainless
steel
needle
valves
led
from
the
low­
pressure
chamber
through
rotameters
and
then
into
the
supply
air
ducts
of
the
three
compartments.
Ammonia
levels
were
measured
daily
by
bubbling
a
known
quantity
of
air
through
dilute
sulfuric
acid
and
back
titrating
with
dilute
sodium
hydroxide.
GLP:
Yes
[
]
No
[
X]
?
[
]
Results:
Coughing
and
nasal,
lacrimal,
and
oral
secretions
were
observed
in
pigs
exposed
for
5
weeks
to
103
and
146
ppm
ammonia.
No
significant
effects
were
seen
at
12
and
61
ppm
for
the
same
exposure
duration.
Test
Substance:
Atmospheric
ammonia
(
NH3)
92
Ammonia
(
CAS
No.
7664­
41­
7)
Remarks:
The
authors
reported
that
ammonia
exposures
(
12­
146
ppm)
caused
a
highly
significant
adverse
effect
upon
feed
consumption
and
average
daily
gain.
However,
no
statistical
evaluations
were
performed
and
furthermore,
the
data
presented
do
not
appear
to
support
these
conclusions.
Reference:
Stombaugh,
D.
P.,
Teague,
H.
S.,
and
Roller,
W.
L.
1969.
Effects
of
atmospheric
ammonia
on
the
pig.
J.
Anim.
Sci.
28(
6):
844­
847.

5.1.3
ACUTE
DERMAL
TOXICITY
Remarks:
No
data
are
available
regarding
systemic
effects
of
dermal
exposure
to
ammonia
or
ammonium
compounds.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.
93
Ammonia
(
CAS
No.
7664­
41­
7)
5.1.4
ACUTE
TOXICITY
BY
OTHER
ROUTES
OF
ADMINISTRATION
(
a)
Type:
LD0
[
];
LD100
[
];
LD50
[
X];
LDL0
[
];
Other
[
]
Species/
strain:
Mus
musculus
(
Albino
mice)
and
Gallus
domesticus
(
White
Leghorn
chicks)
Exposure
Time:
1­
hour
Value:
LD50
(
intravenous;
chicks)
=
2.72
mmol
CH3COONH4/
kg
LD50
(
intravenous;
mice)
=
5.64
mmol
CH3COONH4/
kg
LD50
(
intraperitoneal;
chicks)
=
10.44
mmol
CH3COONH4/
kg
LD50
(
intraperitoneal;
mice)
=
10.84
mmol
CH3COONH4/
kg
Method:
A
total
of
200
chicks
and
180
mice
were
used.
One­
half
to
1.5
mL
isotonic
saline
containing
ammonium
acetate
was
injected
rapidly
into
the
wing
vein
of
the
chicks
and
into
a
tail
vein
of
the
mice
for
intravenous
studies,
and
into
the
peritoneal
cavity
for
the
intraperitoneal
studies.
Ten
animals
were
exposed
to
each
of
eight
to
eleven
concentration
groups.
Animals
were
observed
continually
until
all
signs
of
acute
toxicity
disappeared
and
the
survivors
counted
after
1
hour.
GLP:
Yes
[
]
No
[
]
?
[
X]
Test
substance:
Ammonium
acetate
(
CH3COONH4)
Remarks:
In
the
intravenous
studies,
reaction
began
immediately
in
both
species,
characterized
by
hyperventilation
and
clonic
convulsions.
In
individuals
who
died,
clonic
convulsions
were
followed
by
a
fatal
tonic
extensor
convulsion.
Mice
which
survived
showed
signs
of
hyperventilation
and
hyperirritability,
but
did
not
go
into
a
coma.
Chicks
exhibited
clonic
convulsions
followed
by
a
gradual
onset
of
coma
and
completely
recovered
in
50­
60
minutes.
The
reaction
to
the
intraperitoneal
dose
was
similar
between
the
two
species.
Clonic
convulsions
accompanied
by
a
gradual
onset
of
coma
began
10­
15
minutes
after
injection.
Animals
either
died
of
a
fatal
tonic
extensor
convulsion
or
recovered
in
50­
60
minutes.
Reference:
Wilson,
R.
P.,
Muhrer,
M.
E.,
and
Bloomfield,
R.
A.
1968.
Comparative
ammonia
toxicity.
Comp.
Biochem.
Physiol.
25:
295­
301.

(
b)
Type:
LD0
[
];
LD100
[
];
LD50
[
X];
LDL0
[
];
Other
[
]
Species/
strain:
Rat
Value:
LD50
(
intraperitoneal
dose)
=
8.2
mmol
NH4OCOCH3/
kg
Method:
Intraperitoneal
injection
GLP:
Yes
[
]
No
[
X]
?
[
]
Test
substance:
Ammonia
acetate
Remarks:
Signs
included
hyperventilation,
clonic
convulsions,
coma
and
death.
These
signs
did
not
appear
until
15­
30
minutes
after
administration.
Reference:
Greenstein,
J.
P.,
Winitz,
M.,
Gullino,
P.,
Birnbaum,
S.
M.,
and
Otey,
M.
C.
1956.
Studies
on
the
metabolism
of
amino
acids
and
related
compounds
in
vivo.
III.
Prevention
of
ammonia
toxicity
by
arginine
and
related
compounds.
Arch.
Biochem.
94
Ammonia
(
CAS
No.
7664­
41­
7)
Biophys.
64:
342­
354.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.
(
c)
Type:
LD0
[
];
LD100
[
];
LD50
[
X];
LDL0
[
];
Other
[
]
Species/
strain:
Mouse
Value:
LD50
(
intravenous
dose)
=
6.23
mmol
NH4OCOCH3/
kg
LD50
(
intravenous
dose)
=
5.05
mmol
NH4HCO3/
kg
LD50
(
intravenous
dose)
=
4.47
mmol
NH4CO3/
kg
LD50
(
intravenous
dose)
=
6.75
mmol
NH4Cl/
kg
LD50
(
intravenous
dose)
=
2.53
mmol
NH4OH/
kg
Method:
Intravenous
injection
GLP:
Yes
[
]
No
[
X]
?
[
]
Test
substance:
Ammonium
acetate
(
NH4OCOCH3),
ammonium
bicarbonate
(
NH4HCO3),
ammonium
carbonate
(
NH4CO3),
ammonium
chloride
(
NH4Cl)
and
ammonium
hydroxide
(
NH4OH)
Remarks:
Toxic
signs
included
immediate
hyperventilation
and
clonic
convulsions
followed
by
coma,
tonic
convulsions
and
death.
Surviving
animals
recover
rapidly
and
completely.
Reference:
Warren,
K.
S.
1958.
The
differential
toxicity
of
ammonium
salts.
J.
Clin.
Invest.
37:
497­
501.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

(
d)
Type:
LD0
[
];
LD100
[
];
LD50
[
];
LDL0
[
];
Other
[
X]
Species/
strain:
White
rat
Exposure
Time:
3­
hours
Value:
Minimum
lethal
dose
=
6.80
mL
(
NH4)
2CrO4;
(
0.072
mg
(
NH4)
2CrO4­
N)
Minimum
lethal
dose
=
6.74
mL
NH4Cl;
(
0.072
mg
NH4Cl­
N)
Minimum
lethal
dose
=
6.21
mL
(
NH4)
2HPO4;
(
0.061
mg
(
NH4)
2HPO4­
N)
Minimum
lethal
dose
=
7.87
mL
No.
1
NH4HCO3;
(
0.073
mg
No.
1
NH4HCO3­
N)
Minimum
lethal
dose
=
5.60
mL
No.
2
NH4(
H)
CO3;
(
0.055
mg
No.
2
NH4(
H)
CO3­
N)
Minimum
lethal
dose
=
6.75
mL
NH4NO3;
(
0.065
mg
NH4NO3­
N)
Minimum
lethal
dose
=
10.00
mL
(
NH4)
2SO4;
(
0.094
mg
(
NH4)
2SO4­
N)
Minimum
lethal
dose
=
6.20
mL
NH4Br;
(
0.055
mg
NH4Br­
N)
Minimum
lethal
dose
=
6.30
mL
(
NH4)
2CO3
+
carbamide;
(
0.055
mg
(
NH4)
2CO3
+
carbamide­
N)
Minimum
lethal
dose
=
4.80
mL
(
NH4)
3PO4
neutral;
(
0.0384
mg
(
NH4)
3PO4
neutral­
N)
Minimum
lethal
dose
=
7.56
mL
(
H2)
NH4PO4;
(
0.05708
mg
(
H2)
NH4PO4­
N)
Minimum
lethal
dose
=
6.00
mL
NH4I
Minimum
lethal
dose
=
7.40
mL
NH4CrSO4
Method:
All
salts
(
ammonium
oxalate,
carbonate,
chromate,
chloride,
nitrate,
citrate,
sulfate,
tartrate,
hydrogen
phosphate,
acetate,
95
Ammonia
(
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No.
7664­
41­
7)
sarcolactate,
and
lactate)
were
made
to
approximate
5
percent
solution
and
injected
into
white
rats.
The
nitrogen
in
the
form
of
NH3
was
determined
in
three
5
percent
solutions.
Six
animals
were
used
for
each
series.
GLP:
Yes
[
]
No
[
X]
?
[
]
Test
substance:
Various
ammonium
salts
(
ammonium
oxalate,
carbonate,
chromate,
chloride,
nitrate,
citrate,
sulfate,
tartrate,
hydrogen
phosphate,
acetate,
sarcolactate,
and
lactate)
Remarks:
Contrary
to
previous
reports
by
Rachford
and
Crane
(
1902),
the
current
study
demonstrated
that
the
toxicity
of
ammonium
salts
to
white
rats
is
directly
proportional
to
the
amount
of
un­
ionized
ammonia
present
(
i.
e.
rather
than
on
the
form
of
salt
used).
Note
that
while
this
study
is
old
(
1922),
it
does
provide
evidence
that
the
anion
of
the
salt
is
less
important
with
respect
to
toxicity
than
un­
ionized
ammonia.
Reference:
Underhill,
F.
P.
and
Kapsinow,
R.
1922.
The
comparative
toxicity
of
ammonium
salts.
J.
Biol.
Chem.
54:
451­
457.

5.2
CORROSIVENESS/
IRRITATION
5.2.1
SKIN
IRRITATION/
CORROSION
Remarks:
Ammonia
in
the
form
of
a
gas
or
an
aqueous
solution
is
a
recognized
skin
irritant.
Most
of
the
information
is
human
clinical
data
and
is
described
in
Section
5.11.
Reference:
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

5.2.2
EYE
IRRITATION/
CORROSION
(
a)
Remarks:
Subacute
and
chronic
exposure
of
animals
to
ammonia
in
the
range
of
200­
1000
ppm
has
been
found
to
produce
eye
damage.
At
100­
200
ppm,
moderate
to
severe
eye
irritation
occurred.
Reference:
BIBRA.
1995.
Toxicology
Profile:
Ammonia.
BIBRA
International.

(
b)
Remarks:
Ammonia,
partly
because
of
its
lipid
solubility,
penetrates
the
corneal
membrane
rapidly.
In
rabbits,
conjunctival
oedema
with
ischaemia
and
segmentation
of
the
limbal
vessels
were
noted
within
30
minutes.
In
rabbits
with
corneal
burns,
neovascularization
occurred
after
1
week,
but
it
was
delayed
in
animals
with
both
corneal
and
limbal
burns.
Reference:
National
Research
Council
(
NRC).
1979.
Ammonia.
Subcommittee
on
Ammonia.
Committee
on
Medical
and
Biologic
Effects
of
Environmental
Pollutants.
Division
of
96
Ammonia
(
CAS
No.
7664­
41­
7)
Medical
Sciences,
Assembly
of
Life
Sciences.
National
Research
Council.
Baltimore:
University
Park
Press.
NTIS
No.
PB
278­
027.

5.3
SKIN
SENSITISATION
Remarks:
No
specific
skin
sensitization
studies
were
identified.

*
5.4
REPEATED
DOSE
TOXICITY
(
a)
Species/
strain:
Male
and
female
Sprague­
Dawley
and
Long­
Evans
rats,
male
and
female
Princeton­
derived
Guinea
pigs,
male
New
Zealand
albino
rabbits,
male
squirrel
monkeys
and
purebred
male
beagle
dogs
Route
of
administration:
Inhalation
Exposure
period:
54,
90
or
114­
days
Frequency
of
treatment:
Repeated
dose
or
continuous
exposures
Dose:
155
(
221
ppm)
or
770
(
1100
ppm)
mg/
m3
(
repeated
dose)
127
(
181
ppm),
262
(
374
ppm),
455
(
650
ppm),
470
(
671
ppm)
mg/
m3
(
continuous
dose)
40
mg/
m3
(
continuous
dose)
Control
group:
Yes
[
X];
No
[
];
No
data
[
]
NOEL:
See
results
LOEL:
See
results
Results:
No
mortality
occurred
in
any
species
exposed
to
either
155
or
770
mg/
m3
in
the
repeated
dose
studies.
In
the
770
mg/
m3
exposure,
mild
to
moderate
lacrimation
and
dyspnea
were
evident
in
the
rabbits
and
dogs
in
the
first
week,
but
symptoms
disappeared
by
the
second
week.
In
rats
and
guinea
pigs,
the
lungs
showed
nonspecific
inflammatory
changes.
In
the
continuous
exposure
studies
with
rats,
no
mortality
or
other
treatment
related
effects
were
observed
at
127
mg/
m3.
No
mortality
was
observed
at
262
mg/
m3,
although
about
25%
of
the
rats
had
mild
nasal
discharge.
Continuous
exposure
of
rats
to
455
mg/
m3
resulted
in
the
death
of
50
of
51
rats
by
day
65.
All
showed
mild
signs
of
dyspnea
and
nasal
irritation.
Continuous
exposure
of
rats
to
470
mg/
m3
resulted
in
the
mortality
of
13
of
515
rats
and
4
of
15
guinea
pigs.
Marked
eye
irritation
was
noted
in
dogs
and
rabbits.
In
a
separate
experiment,
all
species
were
exposed
to
a
continuous
concentration
at
40
mg/
m3
for
114
days.
No
mortality
or
signs
of
toxicity
were
noted
in
any
of
the
animals.
Observations
at
necropsy
were
normal,
and
no
treatment
related
histopathological
findings
were
observed.
Method:
In
repeated
exposure
studies,
there
were
30
8­
hr/
day,
5­
day/
week
exposures.
In
the
continuous
exposure
studies,
rats
were
exposed
24
hours/
day
for
90
days.
In
separate
97
Ammonia
(
CAS
No.
7664­
41­
7)
experiments,
all
species
were
exposed
continuously
for
114
days.
The
rate
of
air
flow
to
the
inhalation
chambers
was
maintained
at
1.2
m3/
min
at
a
temperature
of
77
°
F.
GLP:
Yes
[
]
No
[
X]
?
[
]
Test
substance:
Ammonium
hydroxide
(
NH4OH)
Reference:
Coon,
R.
A.,
Jones,
R.
A.,
Jenkins,
Jr.,
L.
J.,
and
Siegel,
J.
1970.
Animal
inhalation
studies
on
ammonia,
ethylene,
glycol,
formaldehyde,
dimethylamine,
and
ethanol.
Toxicol.
Appl.
Pharmacol.
16:
646­
655.

(
b)
Species/
strain:
White
rats
Sex:
Female
[
];
Male
[
];
Male/
Female
[
];
No
data
[
X]
Route
of
administration:
Inhalation
Exposure
period:
3
months
Frequency
of
treatment:
25
or
60­
minutes
every
48­
hours
Dose:
30
and
100
mg/
m3
(
43
and
143
ppm)
Control
group:
Yes
[
X];
No
[
];
No
data
[
]
NOEL:
30
mg/
m3
LOEL:
100
mg/
m3
Results:
At
100
mg/
m3
there
was
mild
leucocytosis.
However,
there
were
no
differences
from
control
with
regard
to
oxygen
consumption,
neuromuscular
excitation
threshold,
heart
rate,
blood
sugar,
blood
residual
nitrogen
and
total
serum
protein.
No
effects
were
reported
at
the
low
dose
level.
GLP:
Yes
[
]
No
[
X]
?
[
]
Test
substance:
Unspecified
ammonia
gas
Reference:
Propkopieva,
A.
S.
and
Yushkov,
G.
G.
1975.
Toxicology
of
ammonia
in
animals
repeatedly
exposed
for
short
lengths
of
time.
Gig.
Tr.
Prof.
Zabol.
(
Russian)
9:
54­
55.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.
In
Clement
Associates,
Inc.
1990.
Health
Effects
Assessment
for
Ammonia.
Prepared
for
The
Fertilizer
Institute,
Washington,
D.
C.

(
c)
Species/
strain:
Sherman
and
Fisher
rats
Sex:
Female
[
];
Male
[
];
Male/
Female
[
];
No
data
[
X]
Route
of
administration:
Inhalation
Exposure
period:
75­
days
Frequency
of
treatment:
Not
specified
Dose:
average
concentration
of
105
mg/
m3
(
150
ppm)
Control
group:
Yes
[
X];
No
[
];
No
data
[
]
NOEL:
See
results
LOEL:
See
results
Results:
Histological
changes
in
the
olfactory
and
respiratory
epithelia
of
the
nasal
cavity
were
similar
for
all
exposed
rats,
showing
increased
thickness,
pyknotic
nuclei,
and
hyperplasia.
The
submucosa
was
oedematous
with
marked
dilation
of
the
small
vessels.
Lesions
decreased
posteriorly.
98
Ammonia
(
CAS
No.
7664­
41­
7)
Method:
Rats
were
exposed
via
inhalation
for
75
days
to
ammonia
from
natural
sources.
GLP:
Yes
[
]
No
[
X]
?
[
]
Test
substance:
Ammonia
gas
(
NH3)
Reference:
Broderson,
J.
R.,
Lindsey,
J.
R.
and
Cranford,
J.
E.
1976.
The
role
of
environmental
ammonia
in
respiratory
mycoplasmosis
of
rats.
Am.
J.
Pathol.
85:
115­
130.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

(
d)
Species/
strain:
Rat
Sex:
Female
[
];
Male
[
X];
Male/
Female
[
];
No
data
[
]
Route
of
administration:
Inhalation
Exposure
period:
Up
to
8­
weeks
Frequency
of
treatment:
Continuous
Dose:
350
mg/
m3
(
500
ppm)
Control
group:
Yes
[
X];
No
[
];
No
data
[
]
NOEL:
See
results
LOEL:
See
results
Results:
The
animals
were
sacrificed
after
different
times
of
exposure
to
establish
a
time
dependency
of
the
effects.
Nasal
irritation
began
on
the
4th
day
of
exposure.
After
3
weeks,
the
exposed
rats
showed
nasal
irritation
and
inflammation
of
the
upper
respiratory
tract
but
no
effects
were
observed
in
the
bronchioles
and
alveoli.
After
8
weeks,
none
of
these
inflammatory
lesions
were
present.
Method:
Twenty
seven
young
male
rats
were
exposed
to
a
continuous
atmospheric
concentration
of
ammonia.
GLP:
Yes
[
]
No
[
X]
?
[
]
Test
substance:
Ammonia
gas
(
NH3)
Reference:
Richard,
D.,
Bouley,
G.
and
Boudene,
C.
1978.
Effets
de
l'inhalation
continue
d'ammoniac
chez
the
rat
et
la
souris.
Bull.
Eur.
Physiopathol.
Resp.
14:
573­
582.
(
Original
in
French.)
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

(
e)
Species/
strain:
Sherman
and
Fisher
rats
Sex:
Female
[
];
Male
[
];
Male/
Female
[
];
No
data
[
X]
Route
of
administration:
Inhalation
Exposure
period:
35­
days
Frequency
of
treatment:
Not
specified
Dose:
175
mg/
m3
(
250
ppm)
Control
group:
Yes
[
X];
No
[
];
No
data
[
]
NOEL:
See
results
LOEL:
See
results
Results:
Histological
changes
in
the
olfactory
and
respiratory
epithelia
of
the
nasal
cavity
were
similar
in
all
exposed
rats,
showing
99
Ammonia
(
CAS
No.
7664­
41­
7)
increased
thickness,
pyknotic
nuclei
and
hyperplasia.
The
submucosa
was
oedematous
with
marked
dilation
of
small
vessels.
Lesions
decreased
posteriorly.
Method:
Rats
were
exposed
via
inhalation
for
35
days
to
purified
ammonia.
GLP:
Yes
[
]
No
[
X]
?
[
]
Test
substance:
Ammonia
gas
(
NH3)
Reference:
Broderson,
J.
R.
et
al.
1976.
Am.
J.
Pathol.
85:
115­
130.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

(
f)
Species/
strain:
Guinea
pig
Sex:
Female
[
];
Male
[
X];
Male/
Female
[
];
No
data
[
]
Route
of
administration:
Inhalation
Exposure
period:
18­
weeks
Frequency
of
treatment:
6
hours/
day;
5
days/
week
Post
exposure
observation
period:
Dose:
About
119
mg/
m3
(
170
ppm)
[
range
98­
140
mg/
m3
(
140­
200)
ppm]
Control
group:
Yes
[
X];
No
[
];
No
data
[
]
NOEL:
See
results
LOEL:
See
results
Results:
There
were
no
significant
findings
after
6
and
12
weeks.
In
animals
sacrificed
after
18
weeks,
there
was
relatively
mild
but
definite
congestion
of
the
liver,
spleen,
and
kidneys
with
degenerative
changes
in
the
adrenal
glands,
and
hemosiderosis
in
the
spleen
indicating
hematotoxicity.
There
was
cloudy
swelling
in
the
epithelium
of
the
proximale
tubules
of
the
kidney
as
well
as
albumin
precipitation
in
the
lumen
with
some
casts.
Method:
Twelve
male
guinea
pigs
(
plus
6
controls)
were
exposed
to
anhydrous
ammonia
gas
released
into
the
air
of
an
exposure
chamber.
GLP:
Yes
[
]
No
[
X]
?
[
]
Test
substance:
Anhydrous
ammonia
gas
(
NH3)
Reference:
Weatherby,
J.
H.
1952.
Chronic
toxicity
of
ammonia
fumes
by
inhalation.
Proc.
Soc.
Exp.
Biol.
Med.
81:
300­
301.

*
5.5
GENETIC
TOXICITY
IN
VITRO
A.
BACTERIAL
TEST
(
a)
Type:
Bacterial
gene
mutation
assay
System
of
testing:
Salmonella
typhimurium
and
E.
coli
Concentration:
Up
to
25,000
ppm
100
Ammonia
(
CAS
No.
7664­
41­
7)
Metabolic
activation:
With
[
];
Without
[
];
With
and
without
[
X];
No
data
[
]
Results:
Cytotoxicity
conc:
With
metabolic
activation:
25,000
ppm
Without
metabolic
activation:
25,000
ppm
Genotoxic
effects:
+
?
­
With
metabolic
activation:
[
]
[
]
[
X]
Without
metabolic
activation:
[
]
[
]
[
X]
Method:
The
cells
were
exposed
to
concentrations
of
the
ammonia
gas
up
to
25,000
ppm.
GLP:
Yes
[
]
No
[
X]
?
[
]
Test
substance:
Ammonia
gas
(
NH3)
Remarks:
With
and
without
liver
metabolic
activation
system.
Reference:
Shimizu
H.
et
al.
1985.
Jap.
J.
Ind.
Health.
27:
400.
(
cited
in
BIBRA
1995).

(
b)
Type:
Bacterial
gene
mutation
assay
System
of
testing:
Salmonella
and
Saccharomyces
Remarks:
WHO
and
Clement
report
that
ammonium
sulfate
was
found
to
be
non­
mutagenic
in
studies
performed
by
Litton
Bionetics,
Inc.
Reference:
Litton
Bionetics,
Inc.
1975.
Mutagenic
Evaluation
of
Compound
FDA
73­
42
Ammonium
Sulfate,
Granular,
Food
Grade,
Washington,
D.
C.
Litton
Bionetics.
(
NTIS
PB­
245506).
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.
Clement
Associates,
Inc.
1990.
Health
Effects
Assessment
for
Ammonia.
Prepared
for
The
Fertilizer
Institute,
Washington,
D.
C.

B.
NON­
BACTERIAL
IN
VITRO
TEST
Type:
Cytogenetic
assay
System
of
testing:
Chick
fibroblasts
Concentration:
Unspecified
Metabolic
activation:
With
[
];
Without
[
];
With
and
without
[
];
No
data
[
X]
Method:
Not
specified
GLP:
Yes
[
]
No
[
X]
?
[
]
Test
substance:
Ammonia
gas
(
NH3)
Remarks:
The
original
reference
reported
that
ammonia
induced
clumping
of
chromosomes,
arrested
spindle
formation,
and
induced
polyploidy
in
chick
fibroblasts.
The
original
study
was
reported
in
1932.
Reference:
Rosenfeld,
M.
1932.
Experimental
modification
of
mitosis
by
ammonia.
Arch.
Exp.
Zellforsch.
Gewebezuecht.
14:
1.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.
101
Ammonia
(
CAS
No.
7664­
41­
7)
*
5.6
GENETIC
TOXICITY
IN
VIVO
Type:
Drosophila
mutagenicity
test
Species/
strain:
Drosophila
melanogaster
Remarks:
WHO,
BIBRA,
and
Clement
all
report
early
studies
suggesting
mutagenic
activity
occurred
upon
exposure
to
ammonia.
However,
this
activity
was
only
evident
at
doses
that
killed
98%
of
the
flies,
which
casts
significant
doubt
on
the
validity
of
the
results.
The
Clement
report
concludes
that
there
are
no
data
to
show
that
ammonia
is
mutagenic
in
mammals.
Reference:
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.
Clement
Associates,
Inc.
1990.
Health
Effects
Assessment
for
Ammonia.
Prepared
for
The
Fertilizer
Institute,
Washington,
D.
C.
BIBRA.
1995.
Toxicology
Profile:
Ammonia.
BIBRA
International.

5.7
CARCINOGENICITY
(
a)
Species/
strain:
Swiss
and
C3H
mice
Sex:
Female
[
];
Male
[
];
Male/
female
[
X];
No
data
[
]
Route
of
administration:
Drinking
water
Exposure
period:
Life­
time
Frequency
of
treatment:
Ad
libitum
Control
group:
Yes
[
];
No
[
];
No
data
[
X];
Concurrent
no
treatment
[
];
Concurrent
vehicle
[
];
Historical
[
]
Results:
Life­
long
ingestion
in
drinking
water
did
not
produce
any
carcinogenic
effects,
and
had
no
effect
on
the
spontaneous
development
of
aderiocarcinoma
of
the
breast
in
C3H
females,
a
characteristic
of
this
strain.
GLP:
Yes
[
]
No
[
X]
?
[
]
Test
substance:
Ammonium
hydroxide
(
NH4OH)
Doses:
Unspecified
Remarks:
The
compound
tested
was
NH4OH,
which
forms
an
equilibrium
with
NH3
when
ammonia
is
dissolved
in
water.
No
further
information
is
given
in
source.
Reference:
Toth,
B.
1972.
Hydrazine,
methylhydrazine
and
methylhydrazine
sulfate
carcinogenesis
in
Swiss
mice.
Int.
J.
Cancer.
9:
109.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

(
b)
Species/
Strain:
All
102
Ammonia
(
CAS
No.
7664­
41­
7)
Remarks:
WHO
and
Clement
note
that
there
is
no
reliable
evidence
indicating
that
ammonia
is
carcinogenic.
BIBRA
notes
a
poorly
reported
study
(
Gaafar
et
al
1992)
in
which
one
of
50
adult
mice
exposed
15
minutes/
day,
6
days/
week
for
8
weeks
to
"
ammonia
vapour"
(
derived
from
a
12%
ammonia
solution,
but
not
further
specified)
developed
nasal
cancer.
However,
there
was
a
low
incidence
of
other
microscopic
changes
in
the
nasal
tissues
suggestive
of
a
tumorigenic
response.
Reference:
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.
Clement
Associates,
Inc.
1990.
Health
Effects
Assessment
for
Ammonia.
Prepared
for
The
Fertilizer
Institute,
Washington,
D.
C.
BIBRA.
1995.
Toxicology
Profile:
Ammonia.
BIBRA
International.

(
c)
Species/
Strain:
Humans
Remarks:
Epidemiological
studies
by
Bittershol
that
have
been
widely
cited,
have
shown
excess
incidence
of
cancers
in
manufacturing
plants.
However,
the
study
has
methodological
flaws
and
confounding
influences
from
carcinogens
in
the
ambient
environment.
Reference:
Bittershol,
G.
(
1971)
Epidemiologic
study
on
cancer
of
workers
in
a
chemical
plant.
Arch.
Gesh.
38:
198­
209
(
Original
in
German).
Clement
Associates,
Inc.
1990.
Health
Effects
Assessment
for
Ammonia.
Prepared
for
The
Fertilizer
Institute,
Washington,
D.
C.

*
5.8
TOXICITY
TO
REPRODUCTION
Type:
Fertility
[
];
One
generation
study
[
X];
Two
generation
study
[
];
Other
[
]
Species/
strain:
Yorkshire
x
Hampshire
x
Chester
White
gilts
(
a
young
sow)
Sex:
Female
[
];
Male
[
];
Male/
Female
[
X];
No
data
[
]
Route
of
Administration:
Inhalation
of
ammonia
in
air
environment
Exposure
Period:
6­
weeks;
10­
weeks
Frequency
of
treatment:
Continuous
Postexposure
observation
period:
Premating
exposure
period:
male:
female:
6
weeks
Duration
of
the
test:
6
weeks
Doses:
mean
7
ppm
NH3,
mean
35
ppm
NH3
Control
group:
Yes
[
];
No
[
X];
No
data
[
];
Concurrent
no
treatment
[
];
Concurrent
vehicle
[
];
Historical
[
]
NOAEL
Prenatal:
See
results
NOAEL
F1
Offspring:
See
results
NOAEL
F2
Offspring:
See
results
103
Ammonia
(
CAS
No.
7664­
41­
7)
Results:
At
the
end
of
two
weeks,
gilts
in
the
moderate
exposure
group
weighed
less
than
those
in
the
low
exposure.
After
2
weeks
gilts
acclimated
and
the
Mean
Daily
Gain
(
MDG)
was
similar
for
the
rest
of
the
experiment.
The
gilts
sacrificed
at
6
weeks
showed
that
the
animals
in
the
low
exposure
were
heavier.
At
day
30
of
gestation,
number
of
fetuses,
fetal
length,
and
fetusto
corpus
luteum
ratio
were
all
similar
between
the
two
groups.
Method:
From
2­
4.5
months
of
age,
gilts
were
exposed
naturally
to
Mycoplasma
hyponeumoniae
and
Pasteurella
multocida,
which
cause
enzootic
pneumonia
and
atrophic
rhinitis,
respectively.
At
4.5
months
of
age,
the
gilts
were
moved
to
one
of
two
rooms
and
exposed
to
either
low
(
mean
7
ppm)
or
moderate
(
mean
36
ppm)
aerial
concentrations
of
ammonia.
Each
exposure
group
consisted
of
40
individuals.
In
the
room
with
low
ammonia
concentration,
manure
was
flushed
weekly
to
maintain
a
0.3
m
depth.
In
the
room
with
moderate
ammonia
concentration,
manure
accumulated
to
0.48
m
depth.
Moderate
aerial
ammonia
concentration
was
obtained
initially
and
maintained
by
adding
anhydrous
ammonia
from
a
steel
tank.
Mean
Daily
Gain
(
MDG)
was
determined
by
weighing
the
gilts
biweekly.
Half
the
gilts
from
each
exposure
concentration
were
sacrificed
after
6
weeks.
The
remaining
gilts
were
maintained
in
their
respective
environments,
exposed
daily
to
mature
boars,
bred
at
first
estrus,
and
sacrificed
at
day
30
of
gestation.
GLP:
Yes
[
]
No
[
]
?
[
X]
Test
substance:
Ammonia
(
NH3)
obtained
by
flushing
of
manure
pits
weekly.
Remarks:
These
data
indicate
that
exposure
of
gilts
to
mean
aerial
ammonia
concentrations
of
36
ppm
depressed
MDG
for
2
weeks
but
failed
to
alter
onset
of
puberty
or
litter
size
through
day
30
of
gestation.
(
relative
to
exposure
to
7ppm).
Reference:
Diekman,
M.
A.,
Scheidt,
A.
B.,
Sutton,
A.
L.,
Green,
M.
L.,
Clapper,
J.
A.,
Kelly,
D.
T.,
and
Van
Alstine,
W.
G.
1993.
Growth
and
reproductive
performance,
during
exposure
to
ammonia,
of
gilts
afflicted
with
pneumonia
and
atrophic
rhinitis.
Am.
J.
Vet.
Res.
54(
12):
2128­
2131.

*
5.9
DEVELOPMENTAL
TOXICITY/
TERATOGENICITY
Remarks:
No
specific
studies
on
developmental
or
teratorgenic
effects
were
identified.

5.10
OTHER
RELEVANT
INFORMATION
A.
SPECIFIC
TOXICITIES
(
NEUROTOXICITY,
IMMUNOTOXICITY
etc.)

B.
TOXICODYNAMICS,
TOXICOKINETICS
(
a)
Type:
Animal
[
X];
Aquatic
[
];
Plant
[
];
Terrestrial
[
];
104
Ammonia
(
CAS
No.
7664­
41­
7)
Other
[
]
Results:
At
the
2
lowest
concentrations,
there
was
no
increase
in
bloodammonia
However,
after
8
hours
at
220
and
826
mg/
m3
unionized
NH3,
significant
increases
of
3.26
and
4.18
mg
NH3/
L
were
noted
indicating
pulmonary
absorption
of
ammonia.
These
levels
declined
over
time
at
higher
concentrations,
suggesting
that
compensation
was
occurring.
Findings
suggest
that
environmental
ammonia
concentrations
found
in
animal
holding
rooms
would
have
minimal
adverse
effects
in
healthy
rats.
Remarks:
Four
groups
of
rats,
8
per
group,
were
exposed
to
average
ammonia
concentrations
of
11,
23,
220
or
826
mg/
m3
(
15,
32,
310,
or
1,157
ppm)
for
24
hours.
Reference:
Schaerdel,
A.
D.,
White,
W.
J.,
Lang,
C.
M.,
Dvorchik,
B.
H.,
and
Bohner,
K.
1983.
Localized
and
systemic
effects
of
environmental
ammonia
in
rats.
Lab.
Anim.
Sci.
33(
1):
40­
45.

(
b)
Type:
Results:
Remarks:
Mucosal
cell
migration
rate
appears
to
be
accelerated
by
longterm
administration
of
0.01%
ammonia,
especially
in
the
antrum.
The
effect
of
ammonia
on
cell
kinetics
differs
between
the
antral
and
body
mucosa.
The
toxicity
of
NH3­
NH4
+
solution
to
rat
gastric
mucosa
was
enhanced
with
an
increase
in
pH
of
the
solution.
The
amount
of
un­
ionized
NH3
increases
with
increasing
pH,
and
it
causes
cell
damage.
In
the
body
mucosa,
which
is
an
acid­
secreting
mucosa,
the
cytotoxicity
of
ammonia
is
lessened
by
secreted
hydrochloric
acid
to
form
NH4Cl.
The
pH
in
the
antral
mucosa
is
higher
than
that
on
the
body
mucosa,
which
may
explain
the
different
effects
of
ammonia
on
the
gastric
mucosal
cell
migration
rate.
References:
Tsujii,
M.,
Kawano,
S.,
Tsuji,
S.,
Ito,
T.,
Nagano,
K.,
Sasaki,
Y.,
Hayashi,
N.,
Fusamoto,
H.,
and
Kamada,
T.
1993.
Cell
kinetics
of
mucosal
atrophy
in
rat
stomach
induced
by
longterm
administration
of
ammonia.
Gastroenterol.
104:
796­
801.

*
5.11
EXPERIENCE
WITH
HUMAN
EXPOSURE
(
a)
Type:
Occupational
exposure
Results:
There
were
no
differences
in
symptoms,
sense
of
smell,
acute
changes
in
lung
function
during
exposure,
or
changes
in
baseline
lung
function
in
exposed
workers
compared
to
control
workers.
There
was
no
relationship
demonstrated
between
the
level
of
exposure
to
ammonia
(
average
of
9.2
ppm)
and
acute
changes
in
lung
function
over
the
work
shift.
No
chronic
effects
on
lung
function
that
could
be
related
to
the
length
of
exposure
were
demonstrated.
105
Ammonia
(
CAS
No.
7664­
41­
7)
Remarks:
The
chronic
effects
of
ammonia
on
industrial
workers
to
levels
less
than
50
ppm
were
assessed.
The
study
also
examined
the
acute
effects
of
ammonia
exposure
on
the
respiratory
system,
eyes,
and
skin.
A
total
of
52
soda
ash
plant
workers,
6
maintenance
workers,
and
35
control
workers
were
assessed.
All
participants
were
male,
mean
age
40.5
years,
and
plant
tenure
approximately
15
years.
Exposed
workers
were
assessed
on
2
workdays;
on
the
first
and
last
workdays
of
their
workweek.
The
study
was
carried
out
over
a
period
of
one
week.
Maintenance
workers
were
assessed
on
one
workday
in
the
middle
of
their
workweek.
The
control
group
was
assessed
twice
on
two
separate
days
during
the
same
week.
All
participants
completed
a
questionnaire
regarding
past
occupational
exposures,
working
conditions,
smoking
history,
and
respiratory
symptoms
and
eye
and
skin
complaints.
Each
participant's
sense
of
smell
was
assessed
at
the
beginning
and
end
of
the
workweek.
Spriometry
was
performed
at
the
beginning
and
end
of
each
work
shift
on
both
test
days
so
that
each
worker
had
four
tests
done.
The
average
concentration
of
ammonia
to
which
workers
were
exposed
during
their
work
shifts
was
determined
using
NIOSH­
recommended
procedures.
Exposed
and
control
workers
were
sampled
over
one
work
shift.
The
average
sample
collection
period
was
8.4
hours.
The
mean
time­
weighted
average
(
TWA)
ammonia
exposure
of
the
exposed
group
was
9.2
ppm,
0.3
ppm
for
the
control
group.
Three
workers
were
exposed
to
TWA
concentrations
in
excess
of
25
ppm.
Reference:
Holness,
D.
G.,
Purdham,
J.
T.,
and
Nethercott,
J.
R.
1989.
Acute
and
chronic
respiratory
effects
of
occupational
exposure
to
ammonia.
Am.
Ind.
Hyg.
Assoc.
J.
50(
12):
646­
650.

(
b)
Type:
Inhalation
exposure
by
human
volunteers
Results:
After
acclimation,
continuous
exposure
to
100
ppm,
with
occasional
excursions
to
200
ppm,
is
easily
tolerated
with
no
observed
effect
on
general
health.
Remarks:
Six
unacclimated
male
and
female
volunteers
were
exposed
six
hours
per
day
over
a
six
week
period
to
concentrations
of
25,
50,
and
100
ppm
ammonia
in
an
industrial
environment,
under
strict
medical
surveillance.
Inurement
to
eye,
nose,
and
throat
irritation
was
demonstrated
after
two
to
three
weeks
in
addition
to
short­
term
subjective
adaptation.
There
were
no
significant
differences
between
subjects
or
controls
on
common
biological
indicators,
in
physical
examinations,
or
in
performance
of
normal
job
duties.
References:
Ferguson,
W.
S.,
Koch,
W.
C.,
Webster,
B.
A.,
and
Gould,
J.
R.
1977.
Human
physiological
response
and
adaptation
to
ammonia.
J.
Occup.
Med.
19(
5):
319­
326.

(
c)
Type:
Inhalation
exposure
by
human
volunteers.
106
Ammonia
(
CAS
No.
7664­
41­
7)
Results:
Concentrations
of
72
ppm
or
higher
caused
discomfort
and
probably
would
not
be
tolerated
by
some
human
subjects.
Concentrations
of
50
ppm
or
less
could
be
tolerated
for
at
least
five
minutes
and
probably
much
longer.
The
level
of
50
ppm
appeared
to
be
below
the
threshold
concentration
causing
discomfort.
Remarks:
Ten
human
subjects
were
exposed
for
five
minutes
to
concentrations
of
32,
50,
72,
or
134
ppm
in
a
dynamic
chamber.
The
results
indicated
irritation
at
72
or
134
ppm
with
more
irritation
at
the
higher
level.
There
was
some
dryness
of
the
nose
in
one
or
two
subjects
exposed
to
32
or
50
ppm,
but
there
was
not
a
definite
irritation
at
these
levels.
Reference:
Keplinger,
M.
L.
1973.
Report
to
International
Institute
of
Ammonia
Refrigeration:
Irritation
Threshold
Evaluation
Study
With
Ammonia.
Industrial
BIOTEST
Laboratories,
Inc.

(
d)
Type:
Inhalation
exposure
by
human
volunteers
Results:
Silverman
et
al.
(
1949)
reported
on
7
human
volunteers
exposed
to
a
concentration
of
300
mg/
m3
(
500
ppm)
ammonia
for
30
min
using
an
oral­
nasal
mask.
All
7
experienced
upper
respiratory
irritation,
which
lasted
up
to
24
hours
in
2
of
the
volunteers.
Two
subjects
experienced
marked
lachrymation,
in
spite
of
the
exposure
being
by
oro­
nasal
mask.
The
average
respiratory
minute
volume
increased
markedly
compared
with
control
values,
and
in
the
5
subjects
in
which
minute­
by­
minute
expired
volumes
were
measured,
there
was
a
marked
cyclical
variation
in
minute
volume
with
a
period
of
4­
7
min.
No
coughing
was
noted.
After
exposure,
respiratory
minute
volumes
fell
to
levels
below
the
pre­
exposure
rate,
but
returned
to
pre­
exposure
values
within
5
min
of
exposure.
Ammonia
retention
decreased
progressively
until
an
equilibrium
of
24%
retention
(
ranging
from
4
to
30%)
was
reached
at
approximately
19
min
(
range
10­
27
min).
The
indices
of
nitrogen
metabolism
(
BUN,
NPN,
urine­
urea,
and
urine­
ammonia)
remained
normal.
The
carbon
dioxide
combining
power
did
not
change.
Remarks:
Seven
male
volunteers
were
exposed
to
350
mg/
m3
(
500
ppm)
NH3
for
30
minutes.
Reference:
Silverman,
L.,
Whittenberger,
J.
L.,
and
Muller,
J.
1949.
Physiological
response
of
man
to
ammonia
in
low
concentrations.
J.
Ind.
Hyg.
Toxicol.
31:
74­
78.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

(
e)
Type:
Inhalation
exposure
by
human
volunteers.
Results:
No
effect
on
vital
capacity
(
VC),
forced
expiratory
volume
[
1
sec]
(
FEV1),
or
forced
inspiratory
volume
[
1
sec]
(
FIV1).
107
Ammonia
(
CAS
No.
7664­
41­
7)
Remarks:
Sixteen
volunteers,
eight
experts
(
29.53
yr)
and
eight
nonexperts
(
students,
18­
30
yr),
were
exposed
for
two
hours
to
ammonia
in
concentrations
of
50,
80,
110
and
140
ppm.
None
of
the
test
subjects
were
considered
to
be
hypersusceptible
to
non­
specific
irritants
or
demonstrated
a
decrease
in
respiratory
function.
Before
leaving
the
test
chamber,
every
subject
described
at
least
one
symptom
as
"
unbearable";
three
times
this
was
irritation
of
throat,
two
times
urge
to
cough,
and
one
time
each
for
smell,
irritation
of
eyes,
nose
or
breast,
headache,
and
general
discomfort.
All
subjects
perceived
a
hypo­
esthesia
of
the
exposed
skin
and
two
noted
excessive
lacrimation.
Reference:
Verberk,
M.
M.
1977.
Effects
of
ammonia
in
volunteers.
Arch.
Occup.
Environ.
Hlth.
39:
73­
81.

(
f)
Remarks:
As
reported
in
NRC
(
1979),
gaseous
ammonia
can
elicit
the
following
effects
at
the
concentration
specified.
400
ppm
(
in
air)
=
Immediate
throat
irritation
700
ppm
(
in
air)
=
Eye
irritation
 
1700
ppm
(
in
air)
=
Coughing
2500
­
6500
ppm
(
in
air)
=
Life
threatening
after
0.5
hour
500
­
10000
ppm
(
in
air)
=
Mortality
Reference:
National
Research
Council
(
NRC).
1979.
Ammonia.
Subcommittee
on
Ammonia.
Committee
on
Medical
and
Biological
Effects
of
Pollutants.
Division
of
Medical
Sciences,
Assembly
of
Life
Sciences.
National
Research
Council.
Baltimore:
University
Park
Press.
NTIS
No.
PB
278­
027.
108
Ammonia
(
CAS
No.
7664­
41­
7)
6.
REFERENCES
29
CFR
1910.1000.
June
1,
1998.
In
Hazardous
Substances
Database
(
HSDB).
Ammonia.
1999.
National
Library
of
Medicine,
Bethesda,
MD.

Admiraal,
W.
1977.
Tolerance
of
estuarine
benthic
diatoms
to
high
concentrations
of
ammonia,
nitrite
ion,
nitrate
ion,
and
orthophosphate.
Mar.
Biol.
43:
307­
315.

Anderson,
D.
P.,
Beard,
C.
W.,
and
Hanson,
R.
P.
1964.
The
adverse
effects
of
ammonia
on
chickens
including
resistance
to
infection
with
newcastle
disease
virus.
Avian
Dis.
8:
369­
379.

Ankley,
G.
T.,
Schubauer­
Berigan,
and
Monson,
P.
D.
1995.
Influence
of
pH
and
hardness
on
toxicity
of
ammonia
to
the
amphipod
Hyalella
azteca.
Can.
J.
Fish.
Aquat.
Sci.
52:
2078­
2083.

Anthonisen,
A.
C.,
Loehr,
R.
C.,
Prakasam,
T.
B.
S.,
and
Srinath,
E.
G.
1976.
Inhibition
of
nitrification
by
ammonia
and
nitrous
acid.
J.
Water
Poll.
Control
Fed.
48:
835­
852.

Appelman,
L.
M.,
ten
Berge,
W.
F.,
and
Reuzel,
P.
G.
J.
1982.
Acute
inhalation
toxicity
study
of
ammonia
in
variable
exposure
periods.
Am.
Ind.
Hyg.
Assoc.
J.
43(
9):
662­
665.

Arthur,
J.
W.,
West,
C.
W.,
Allen,
K.
N.,
and
Hedtke,
S.
F.
1987.
Seasonal
toxicity
of
ammonia
to
five
fish
and
nine
invertebrate
species.
Environ.
Contam.
Toxicol.
38:
324­
331.

Asman,
W.
A.
and
Janssen,
A.
J.
1987.
A
long­
range
transport
model
for
ammonia
and
ammonium
for
Europe.
Atmos.
Environ.
21(
10).
In
Constable,
M.,
Jensen,
F.,
McLernon,
J.
Craig,
G.,
Moore,
D.
1999.
Canadian
Environmental
Protection
Act,
Priority
Substances
List
II:
Supporting
document
for
Ammonia
in
the
Aquatic
Environment.
Draft,
Unpublished
Version.
Government
of
Canada,
Environment
Canada.

Asman,
W.
A.,
Pinksterboer,
E.
F.,
Mass,
H.
F.,
Erisman,
J.
W.,
Waijers­
Ypelaan,
A.,
Slanina,
J.,
and
Horst,
T.
W.
1989.
Gradients
of
the
ammonia
concentration
in
a
nature
reserve:
Model
results
and
measurements.
Atmos.
Environ.
23(
10):
2259.
In
Constable,
M.,
Jensen,
F.,
McLernon,
J.,
Craig,
G.,
Moore,
D.
1999.
Canadian
Environmental
Protection
Act,
Priority
Substances
List
II:
Supporting
Document
for
Ammonia
in
the
Aquatic
Environment.
Draft,
Unpublished
Version.
Government
of
Canada,
Environment
Canada.

Austin,
E.
R.
and
Lee,
G.
F.
1973.
Nitrogen
release
from
lake
sediments.
J.
Water
Pollut.
Control
Fed.
45:
870­
879.

Bader,
J.
A.
and
Grizzle,
J.
M.
1992.
Effects
of
ammonia
on
growth
and
survival
of
recently
hatched
channel
catfish.
Amer.
Fish.
Soc.
4:
17­
23.

Baker,
J.
H.
et
al.
1959.
Determination
of
application
losses
of
anhydrous
ammonia.
Agronomy
Journal
51:
361­
362.
In
U.
S.
Environmental
Protection
Agency
(
USEPA).
1998b.
Emission
Factor
Documentation
for
AP­
42
Section
9.2.1.
Draft.
Research
Triangle
Park:
Office
of
Air
Quality
Planning
and
Standards.
109
Ammonia
(
CAS
No.
7664­
41­
7)
Barrow,
C.
S.,
Alarie,
Y.
and
Stock,
M.
F.
1978.
Sensory
irritation
and
incapacitation
evoked
by
thermal
decomposition
products
of
polymers
and
comparisons
with
known
sensory
irritants.
Arch.
Environ.
Health.
33:
79­
88.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Barton,
L.
V.
1940.
Toxicity
of
ammonia,
chlorine,
hydrogen
cyanide,
hydrogen
sulphide,
and
sulphur
dioxide
gases.
IV.
Seeds.
Contrib.
Boyce
Thompson
Inst.
11:
357­
363.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

BASF
AG,
Abteilung
Analytik;
unveroeffentlichte
Untersuchung
(
BRU
92.004).
In
European
Commission
1996.
Ammonia.
International
Uniform
Chemical
Information
Database.

BASF
AG,
Sicherheitsdatenblatt
Ammoniak
fluessig
(
27.04.94).
In
European
Commission.
1996.
Ammonia.
International
Uniform
Chemical
Information
Database.

Beaumont,
A.
R.
and
Budd,
M.
D.
1984.
Marine.
Poll.
Bull.
15:
402­
405.
In
Vorlaeufige
Ergebnisse
des
F+
E­
Vorhabens
Bewertung
wassergefaehrdender
Stoffe.
1985.
vgl.
Datenblaetter
zum
Katalog
wassergefaehrdender
Stoffee,
KBwS,
Nr.
5.
In
European
Commission.
1996.
Ammonia.
International
Uniform
Chemical
Information
Database.

Benedict,
H.
M.
and
Breen,
W.
H.
1955.
The
use
of
weeds
as
a
means
of
evaluating
vegetation
damage
caused
by
air
pollution.
Proc.
3rd
Natl.
Pollution
Symp.
Pasadena,
Calif.
Pp.
177­
190.
Zitiert
nach:
DSM,
Inventory
of
data
on
chemicals,
Ammonia,
Delft,
1992.
RO471001/
4150J.
In
European
Commission.
1996.
Ammonia.
International
Uniform
Chemical
Information
Database.

Betterton,
E.
A.
1992.
In
Nriagu,
J.
O.
(
ed.).
In
Gaseous
Pollutants:
Characterization
and
Cycling.
New
York:
John
Wiley
&
Sons,
Inc.
Pp.
1­
50.

BIBRA.
1995.
Toxicology
Profile:
Ammonia.
BIBRA
International.

Bittershol,
G.
1971.
Epidemiologic
study
on
cancer
of
workers
in
a
chemical
plant.
Arch.
Gesh.
38:
198­
209
(
Original
in
German).

Booth,
N.
H.
and
McDonald,
L.
E.
1982.
Veterinary
Pharmacology
and
Therapeutics.
5th
edition.
Pp.
938­
939.

Boyd,
E.
M.,
MacLachlan,
M.
L.,
and
Perry,
W.
F.
1944.
Experimental
ammonia
gas
poisoning
in
rabbits
and
cats.
J.
Ind.
Hyg.
Toxicol.
26:
29­
34.

Broderson,
J.
R.,
Lindsey,
J.
R.
and
Cranford,
J.
E.
1976.
The
role
of
environmental
ammonia
in
respiratory
mycoplasmosis
of
rats.
Am.
J.
Pathol.
85:
115­
130.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Brown,
V.
M.;
Jordan,
D.
H.
M.;
and
Tiller,
B.
A.
1969.
The
acute
toxicity
to
rainbow
trout
of
fluctuating
concentrations
and
mixtures
of
ammonia,
phenol
and
zinc.
J.
Fish.
Biol.
1:
1­
9.
110
Ammonia
(
CAS
No.
7664­
41­
7)
Buckley,
J.
A.
1978.
Acute
toxicity
of
un­
ionized
ammonia
to
fingerling
coho
salmon.
Prog.
Fish­
Cult.
40(
1):
30­
32.

Budavari,
S.
(
ed.).
1996.
Merck
Index.
12th
ed.
Whitehouse
Station:
Merck
Research
Laboratories.

Burkhalter,
D.
E.
and
Kaya,
C.
M.
1977.
Effects
of
prolonged
exposure
to
ammonia
on
fertilized
eggs
and
sac
fry
of
rainbow
trout
(
Salmo
gairdneri).
Trans.
Amer.
Fish.
Soc.
106(
5):
470­
474.

Calamari,
D.,
Marchetti,
R.,
and
Vailati,
G.
1981.
Effects
of
long­
term
exposure
to
ammonia
on
the
developmental
stages
of
rainbow
trout
(
Salmo
gairdneri
Richardson).
Rapp.
P.­
v.
Reun.
Cons.
Int.
Explor.
Mer.
178:
81­
86.

Cameron,
J.
N.
and
Heisler,
N.
1983.
Studies
of
ammonia
in
the
rainbow
trout:
physicochemical
parameters,
acid­
base
behaviour
and
respiratory
clearance.
J.
Exp.
Biol.
105:
107­
125.

Canadian
Fertilizer
Institute
(
CFI).
1996.
1995
Emissions
inventory,
update.
Ottawa.
In
Constable,
M.,
Jensen,
F.,
McLeron,
J.
Craig,
G.,
Moore
D.
1996.
Canadian
Environmental
Protection
Act,
Priority
Substances
List
II:
Supporting
Document
for
Ammonia
in
the
Aquatic
Environment.
Draft,
Unpublished
Version.
Government
of
Canada,
environment
Canada.

Charles,
D.
R.
and
Payne,
C.
G.
1966.
The
influence
of
graded
levels
of
atmospheric
ammonia
on
chickens.
II.
Effects
on
the
performance
of
laying
hens.
Br.
Poult.
Sci.
7:
189­
198.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Chen,
J.
C.
and
Kou,
Y.
Z.
1993.
Accumulation
of
ammonia
in
the
haemolymph
of
Penaeus
monodon
exposed
to
ambient
ammonia.
Aquaculture
109:
177­
185.

Clement
Associates,
Inc.
1990.
Health
Effects
Assessment
for
Ammonia.
Prepared
for
The
Fertilizer
Institute,
Washington,
D.
C.

Coffee,
R.
C.
and
Bartholomew,
W.
V.
1964.
Some
aspects
of
ammonia
sorption
by
soil
surfaces.
Soil
Sci.
Soc.
Proc.
28:
485­
490.

Colt,
J.
and
Tchobanoglous,
G.
1978.
Chronic
exposure
of
channel
catfish
Ictalurus
punctatus,
to
ammonia:
effects
on
growth
and
survival.
Aquaculture
15:
353­
372.

Compressed
Gas
Association,
Inc.
1990.
Handbook
of
Compressed
Gases.
3rd
ed.
Pp.
259­
260.
In
Compressed
Gas
Association,
Inc.
1999.
American
National
Standard
Safety
Requirements
for
the
Storage
and
Handling
of
Anhydrous
Ammonia.
5th
ed.
Arlington:
Compressed
Gas
Association,
Inc.
111
Ammonia
(
CAS
No.
7664­
41­
7)
Constable,
M.,
Jensen,
F.,
McLeron,
J.
Craig.,
G.,
Moore,
D.
1999.
Canadian
Environmental
Protection
Act,
Priority
Substances
List
II:
Supporting
Document
for
Ammonia
in
the
Aquatic
Environment.
Draft,
Unpublished
Version.
Government
of
Canada,
Environment
Canada.

Coon,
R.
A.,
Jones,
R.
A.,
Jenkins,
Jr,
L.
J.,
and
Siegel,
J.
1970.
Animal
inhalation
studies
on
ammonia,
ethylene,
glycol,
formaldehyde,
dimethylamine,
and
ethanol.
Toxicol.
Appl.
Pharmacol.
16:
646­
655.

Dabrowski,
H.
and
Skiora,
H.
1986.
Acute
toxicity
of
ammonia
to
common
carp
(
Cyprinus
carpioL.).
Pol.
Arch.
Hydrobiol.
33(
1):
121­
128.

Day,
D.
L.,
Hansen,
E.
L,
and
Anderson,
S.
1965.
Gases
and
odors
in
confinement
swine
buildings.
Trans.
Am.
Soc.
Agric.
Eng.
8:
118­
121.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Denmead,
O.
T.
et
al.
1982.
Atmospheric
dispersion
of
ammonia
during
application
of
anhydrous
ammonia
fertilizer.
J.
Environ.
Qual.
11(
4):
568­
572.
In
U.
S.
Environmental
Protection
Agency
(
USEPA).
1998a.
AP­
42.
Draft.
Vol.
1
5th
ed.
Research
Triangle
Park:
Office
of
Air
Quality
Planning
and
Standards.

Denmead,
O.
T.
et
al.
1977.
A
direct
field
measurement
of
ammonia
emission
after
injection
of
anhydrous
ammonia.
Soil
Sci.
Soc.
Amer.
J.
41:
1001­
1004.
In
U.
S.
Environmental
Protection
Agency
(
USEPA).
1998a.
AP­
42.
Draft.
Vol.
1
5th
ed.
Research
Triangle
Park:
Office
of
Air
Quality
Planning
and
Standards.

Diamond,
J.
M.,
Mackler,
D.
G.,
Rasnake,
W.
J.,
and
Gruber,
D.
1993.
Derivation
of
site­
specific
ammonia
criteria
for
an
effluent­
dominated
headwater
stream.
Environ.
Toxicol.
Chem.
12:
649­
658.

Diekman,
M.
A.,
Scheidt,
A.
B.,
Sutton,
A.
L.,
Green,
M.
L.,
Clapper,
J.
A.,
Kelly,
D.
T.,
and
Van
Alstine,
W.
G.
1993.
Growth
and
reproductive
performance,
during
exposure
to
ammonia,
of
gilts
afflicted
with
pneumonia
and
atrophic
rhinitis.
Am.
J.
Vet.
Res.
54(
12):
2128­
2131.

Directive
92/
32
of
April
30,
1992.
1992.
Official
Journal
of
the
European
Communities,
No.
L154
of
June
5,
1992.

Dodd,
K.
T.
and
Gross.
D.
R.
1980.
Ammonia
inhalation
toxicity
in
cats:
a
study
of
acute
and
chronic
respiratory
dysfunction.
Arch.
Environ.
Health.
35(
1):
6­
14.

DSM,
Inventory
of
Data
on
chemicals,
Ammonia,
Delft.
1992.
LRO471001/
4150J.
In
European
Commission.
1996.
Ammonia.
International
Uniform
Chemical
Information
Database.

Ecological
Analysts,
Inc.
1981.
The
Sources,
Chemistry,
Fate,
and
Effects
of
Ammonia
in
Aquatic
Environments.
Washington
DC:
American
Petroleum
Institute.

Emerson,
K.,
Russo,
R.
C.,
Lund,
R.
E.,
and
Thurston,
R.
V.
1975.
Aqueous
ammonia
equilibrium
calculations:
effect
of
pH
and
temperature.
J.
Fish.
Res.
Board
Can.
32:
2379­
2383.
112
Ammonia
(
CAS
No.
7664­
41­
7)
Environment
Canada.
1984.
Technical
Information
for
Problem
Spills:
Ammonia.
Ottawa,
Ontario:
Environmental
Protection
Service,
Technical
Services
Branch.

Epifanio,
C.
E.
and
Srna,
R.
F.
1975.
Toxicity
of
ammonia,
nitrite
ion,
nitrate
ion,
and
orthophosphate
to
Mercenaria
mercenaria
and
Crassostrea
virginica.
Mar.
Biol.
33:
241­
246.

Erickson,
R.
J.
1985.
An
evaluation
of
mathematical
models
for
the
effects
of
pH
and
temperature
on
ammonia
toxicity
to
aquatic
organisms.
Water
Res.
19(
8):
1047­
1058.

European
Commission.
1996.
Ammonia.
International
Uniform
Chemical
Information
Database.

Evans,
D.
E.
and
Cameron,
J.
N.
1956.
Gi11
ammonia
transport.
J.
Exp.
Zool.
239:
17­
23.

Fenn,
L.
and
Hossner,
L.
1985.
Ammonia
volatilization
from
ammonia
or
ammonium
forming
nitrogen
fertilizers.
In
Advances
in
Soil
Science.
Vol.
I.
New
York:
Springer
Verlag.
In
Constable,
M.,
Jensen,
F.,
McLernon,
J.
Craig,
G.,
Moore,
D.
1999.
Canadian
Environmental
Protection
Act.,
Priority
Substances
List
II:
Supporting
Document
for
Ammonia
in
the
Aquatic
Environment.
Draft,
Unpublished
Version.
Government
of
Canada,
Environment
Canada.

Ferguson,
W.
S.,
Koch,
W.
C.,
Webster,
L.
B.,
and
Gould,
J.
R.
1977.
Human
physiological
response
and
adaptation
to
ammonia.
J.
Occup.
Med.
19(
5):
319­
326.

Fromm,
P.
O.,
and
Gillette,
J.
R.
1968.
Effect
of
ambient
ammonia
on
blood
ammonia
and
nitrogen
excretion
of
rainbow
trout
(
Salmo
gaidneri).
Comp.
Biochem.
Physiol.
26:
887­
896.

Fuhrer,
J.
1985.
Formation
of
secondary
air
pollutants
and
their
occurrence
in
Europe.
Experientia
(
Basel)
41:
286­
301.

Gersich,
F.
M.
and
Hopkins,
D.
L.
1986.
Site­
specific
acute
and
chronic
toxicity
of
ammonia
to
Daphnia
magna
Straus.
Environ.
Toxicol.
Chem.
5:
443­
447.

Graedel,
T.
E.
and
Allara,
D.
L.
1977.
Pp.
467­
473.
EPA­
600/
3­
77­
001A,
PB­
264.

Greenstein,
J.
P.,
Winitz,
M.,
Gullino,
P.,
Birnbaum,
S.
M.,
and
Otey,
M.
C.
1956.
Studies
on
the
metabolism
of
amino
acids
and
related
compounds
in
vivo.
III.
Prevention
of
ammonia
toxicity
by
arginine
and
related
compounds.
Arch.
Biochem.
Biophys.
64:
342­
354.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Hales,
J.
M.
and
Drewes,
D.
R.
1979.
Solubility
of
ammonia
in
water
at
low
concentrations.
Atmos.
Environ.
13:
1133­
1147.

Harader,
R.
R.
and
Allen,
G.
H.
1983.
Ammonia
toxicity
to
chinook
salmon
parr:
reduction
in
saline
water.
Trans.
Amer.
Fish.
Soc.
112:
834­
837.
113
Ammonia
(
CAS
No.
7664­
41­
7)
Hazardous
Substances
Data
Bank
(
HSDB).
1999.
Ammonia.
National
Library
of
Medicine,
Bethesda,
MD.

Hermanutz,
R.
O.,
Hedtke,
S.
F.,
Arthur,
J.
W.,
Andrew,
R.
W.,
Allen,
K.
N.,
and
Helgen,
J.
C.
1987.
Ammonia
effects
on
microinvertebrates
and
fish
in
outdoor
experimental
streams.
Environ.
Poll.
47:
249­
283.
Hickey,
C.
W.
and
Vickers,
M.
L.
1994.
Toxicity
of
ammonia
to
nine
native
New
Zealand
freshwater
invertebrate
species.
Arch.
Environ.
Contam.
Toxicol.
26:
292­
298.

Holness,
D.
G.,
Purdham,
J.
T.,
and
Nethercott,
J.
R.
1989.
Acute
and
chronic
respiratory
effects
of
occupational
exposure
to
ammonia.
Am.
Ind.
Hyg.
Assoc.
J.
50(
12):
646­
650.

Hutchinson,
G.
L.
and
Viets,
F.
G.,
Jr.
1969.
Nitrogen
enrichment
of
surface
water
by
absorption
of
ammonia
volatilized
from
cattle
feedlots.
Science
166:
514­
515.

IFDC.
2002.
North
America
Fertilizer
Capacity,
January
2002.

Indorato,
A.
M.
et
al.
1985.
First
International
Symposium
on
Toxicity
Testing
using
Bacteria.
Burlington,
Ontario,
Canada
17­
19
May
1983.
In
Datenblaetter
zum
Katalog
wassergefaehrdender
Stoffe,
KBwS.
Nr.
5.
In
European
Commission.
1996.
Ammonia.
International
Uniform
Chemical
Information
Database.

Jofre,
M.
B.,
and
Karasov,
W.
H.
1999.
Direct
effect
of
ammonia
on
three
species
of
North
American
anuran
amphibians.
Environ.
Toxicol.
Chem.
18(
8):
1806­
1812.

Jones,
K.
1973.
Ammonia.
In
Bailar,
J.
C.,
Jr.,
Emeleus,
H.
J.,
Nyholm,
R.
and
Trotman­
Dickenson,
A.
F.,
(
ed.).
Comprehensive
Inorganic
Chemistry.
New
York:
Pergammon
Press.
2,
Pp.
199­
227.

Kapeghian,
J.
C.,
Mincer,
H.
H.,
Jones,
A.
B.,
Verlangieri,
A.
J.,
and
Waters,
I.
W.
1982.
Acute
inhalation
toxicity
of
ammonia
in
mice.
Bull.
Environm.
Contam.
Toxicol.
29:
371­
378.

Keeney,
D.
R.
1972.
The
Fate
of
Nitrogen
in
Aquatic
Ecosystems.
Literature
Review
No.
3.
Madison:
University
of
Wisconsin
Water
Resources
Center.

Keplinger,
M.
L.
1973.
Report
to
International
Institute
of
Ammonia
Refrigeration:
Irritation
Threshold
Evaluation
Study
with
Ammonia.
Industrial
BIOTEST
Laboratories,
Inc.

Keyes,
W.
F.
1975.
Nitrogen.
In
Mineral
facts
and
problems.
Washington
DC:
US
Department
of
the
Interior.
P.
749­
760.
In
World
Health
Organization
(
WHO).
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Kloppic,
E.,
Jasobasch,
G.,
and
Rapoprt,
S.
1967.
Enhancement
of
the
glycolytic
rate
by
action
of
ammonium
ion
on
phosphofructokinase
activity.
Original
in
German.
Acta.
Biol.
Med.
18:
37­
42.
In
Constable,
M.,
Jensen,
F.,
McLernon,
J.
Craig,
G.,
Moore,
D.
1999.
Canadian
Environmental
Protection
Act,
Priority
Substances
List
II:
Supporting
Document
for
114
Ammonia
(
CAS
No.
7664­
41­
7)
Ammonia
in
the
Aquatic
Environment.
Draft,
Unpublished
Version.
Government
of
Canada,
Environment
Canada.

Knepp,
G.
L.
and
Arkin,
G.
F.
1973.
Ammonia
toxicity
levels
and
nitrate
tolerance
of
channel
catfish.
Prog.
Fish­
Cult.
35(
4):
221­
224.

Knoph,
M.
B.
1992.
Acute
toxicity
of
ammonia
to
Atlantic
Salmon
(
Salmo
salar)
parr.
Comp.
Biochem.
Physiol.
101C(
2):
275­
282.

Landfair,
S.
W.,
Brown,
E.
L.,
Israel,
R.
J.,
and
Kreis,
P.
B.
1993.
The
EC's
seventh
amendment:
Fundamental
changes
in
the
regulation
of
chemical
substances
manufactured
and
imported
into
the
European
community.
International
Environmental
Reporter
0149­
8738/
93:
785­
790.

Langowska,
I.,
and
Moskal,
J.
1974.
Effect
of
ammonia
and
urea
on
nitrobacteria
in
water
environment.
Pol.
Arch.
Hydrobiol.
21(
1):
119­
123.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Lide,
D.
R.
(
ed.).
1999.
CRC
Handbook
of
Chemistry
and
Physics.
80th
Edition.
Boston:
CRC
Press.

Linden,
E.,
Bengtsson,
B.
E.,
Svanberg,
O.,
and
Sundstrom,
G.
1979.
The
acute
toxicity
of
78
chemicals
and
pesticide
formulations
against
two
brackish
water
organisms,
the
bleak
(
Alburnus
alburnus)
and
the
harpacticoid
(
Nitocra
spinipes).
Chemosphere
11/
12:
843­
851.

Litton
Bionetics,
Inc.
1975.
Mutagenic
Evaluation
of
Compound
FDA
73­
42
Ammonium
Sulfate,
Granular,
Food
Grade,
Washington,
D.
C.
Litton
Bionetics.
(
NTIS
PB­
245506).
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

MacEwen,
J.
D.
and
Vernot,
E.
H.
1972.
Toxic
hazards
research
unit
annual
technical
report.
NTIS
AD
755­
358.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Mackay,
D.,
Shiu,
W.
Y.,
and
Ma,
K.
C.
1994.
Level
I,
II
and
III
Fugacity
Calculations
for
the
Illustrated
Handbook
for
Physical­
chemical
Properties
and
Environmental
Fate
for
Organic
Chemicals.
New
York:
CRC
Press.

Marsalek,
J.,
and
Ng,
H.
Y.
F.
1989.
Evaluation
of
pollution
loadings
from
urban
nonpoint
sources:
Methodology
and
applications.
J.
Great
Lakes
Res.
15(
3):
444­
451.
In
Constable,
M.,
Jensen,
F.,
McLernon,
J.
Craig,
G.,
Moore,
D.
1999.
Canadian
Environmental
Protection
Act,
Priority
Substances
List
II:
Supporting
Document
for
Ammonia
in
the
Aquatic
Environment.
Draft,
Unpublished
Version.
Government
of
Canada,
Environment
Canada.
Government
of
Canada,
Environment
Canada.

Mayan,
M.
H.
and
Merilan,
C.
P.
1972.
Effects
of
ammonia
inhalation
on
respiration
rate
of
rabbits.
J.
Anim.
Sci.
34(
3):
447­
452.
115
Ammonia
(
CAS
No.
7664­
41­
7)
Mayes,
M.
A.,
Alexander,
H.
C.,
Hopkins,
D.
L.,
and
Latvaitas,
P.
B.
1986.
Acute
and
chronic
toxicity
of
ammonia
to
freshwater
fish:
a
site­
specific
study.
Environ.
Toxicol.
Chem.
5:
437­
442.

McCormick,
J.
H.,
Broderius,
S.
J.,
and
Fiandt,
J.
T.
1984.
Toxicity
of
ammonia
to
early
life
stages
of
the
green
sunfish
Lepomis
cyanellus.
Environ.
Poll.
(
Series
A)
36:
147­
163.

Miller,
D.
C.,
Poucher,
S.,
Cardin,
J.
A.,
and
Hansen,
D.
1990.
The
acute
and
chronic
toxicity
of
ammonia
to
marine
fish
and
a
mysid.
Arch.
Environ.
Contam.
Toxicol.
19:
40­
48.

Monda,
D.
P.,
Galat,
D.
L.,
Finger,
S.
E.,
and
Kaiser,
M.
S.
1995.
Acute
toxicity
of
ammonia
(
NH3­
N)
in
sewage
effluent
to
Chironomus
riparius:
II.
Using
a
generalized
linear
model.
Arch.
Environ.
Contam.
Toxicol.
28:
385­
390.

Mosier,
A.
R.
1978.
Inhibition
of
photosynthesis
and
nitrogen
fixation
in
algae
by
volatile
nitrogen
bases.
J.
Environ.
Qual.
7(
2):
237­
240.

Mount,
D.
I.
and
Norberg,
T.
J.
1984.
A
seven­
day
life­
cycle
cladoceran
toxicity
test.
Environ.
Toxicol.
Chem.
3:
425­
434.

National
Institute
for
Occupational
Safety
and
Health
(
NIOSH).
1997.
NIOSH
Pocket
Guide
to
Chemical
Hazards
P.
359
National
Research
Council
(
NRC).
1979.
Ammonia.
Subcommittee
on
Ammonia.
Committee
on
Medical
and
Biologic
Effects
of
Environmental
Pollutants.
Division
of
Medical
Sciences,
Assembly
of
Life
Sciences.
National
Research
Council.
Baltimore:
University
Park
Press.
NTIS
No.
PB
278­
027.

Neufeld,
R.
D.,
Hill,
A.
J.,
and
Adekoya,
D.
O.
1980.
Phenol
and
free
ammonia
inhibition
to
Nitrosomonas
activity.
Water
Res.
14(
12):
1695­
1703.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Nimmo,
D.
W.
R.,
Link,
D.,
Parrish,
L.
P.,
Rodriguez,
G.
J.,
and
Wuerthele,
W.
1989.
Comparison
of
on­
site
and
laboratory
toxicity
tests:
derivation
of
site­
specific
criteria
for
un­
ionized
ammonia
in
a
Colorado
transitional
stream.
Environ.
Toxicol.
Chem.
8:
1177­
1189.

OECD.
1997.
SIDS
Manual.
OECD
Secretariat,
3rd
Revision,
July
1997.

Overrein,
L.
N.,
Seip,
H.
M.,
and
Tollan,
A.
(
eds.).
1980.
Acid
Precipitation:
Effects
on
Forest
and
Fish.
Final
Report.
Oslo.
SNSF
Project
1972­
1980.

Person­
LeRuyet,
J.,
Boeuf,
G.
Zambonin­
Infant,
J.,
Helgason,
S.,
and
Le
Roux,
A.
1998.
Short­
term
physiological
changes
in
turbot
and
seabream
juveniles
exposed
to
exogenous
ammonia.
Comp.
Biochem.
Physiol.
119A:
511­
518.
116
Ammonia
(
CAS
No.
7664­
41­
7)
Powers,
W.
L.,
Terry,
R.
V.,
and
Murphy,
L.
S.
1977.
Fate
of
nitrogen
from
manure
disposal.
In
Proceedings
of
the
National
Conference
on
Disposal
of
Residues
on
Land.
Pp.
156­
160.

Propkopieva,
A.
S.
and
Yushkov,
G.
C.
1975.
Toxicology
of
ammonia
in
animals
repeatedly
exposed
for
short
lengths
of
time.
Gig.
Tr.
Prof.
Zabol.
9:
54­
55.
(
Russian).
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Propkopieva,
A.
S.
et
al.
1973.
Materials
for
a
toxicological
characteristic
of
the
long­
term
effect
of
ammonia
on
the
organisms
of
animals
after
brief
exposure.
Gig.
Tr.
Prof.
Zabol.
6:
56­
57.
(
Russian).
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Qureshi,
A.
A.,
Flood,
K.
W.,
Thompson,
S.
R.,
Janhurst,
S.
M.,
Iniss,
C.
S.,
and
Rokosh,
D.
A.
1982.
Comparison
of
luminescent
bacterial
test
with
other
bioassays
for
determining
toxicity
of
pure
compounds
and
complex
effluents.
Aquatic
Toxicology
and
Hazard
Assessment:
Fifth
Conference,
ASTM
STP
766.
Pearson,
J.
G.,
Foster,
R.
B.,
and
Bishop,
W.
E.
(
eds.).
American
Society
for
Testing
and
Materials
(
ASTM).
Pp.
179­
195.

Randall,
D.
J.,
and
Wicks,
B.
J.
1999.
Fish:
ammonia
production,
excretion,
and
toxicity.
In
press.
Paper
presented
in
the
Fifth
International
Symposium
on
Fish
Physiology,
Toxicology,
and
Water
Quality.
University
of
Hong
Kong.

Rice,
S.
D.
and
Bailey,
J.
E.
1980.
Survival,
size,
and
emergence
of
pink
salmon,
Oncorhynchus
gorbuscha,
alevins
after
short­
and
long­
term
exposures
to
ammonia.
Fish.
Bull.
78(
3):
641­
648.

Richard,
D.,
Bouley,
G.
and
Boudene,
C.
1978.
Effets
de
l'inhalation
continue
d'ammoniac
chez
the
rat
et
la
souris.
Bull.
Eur.
Physiopathol.
Resp.
14:
573­
582.
(
Original
in
French).
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Richardson,
J.
1997.
Acute
ammonia
toxicity
for
eight
New
Zealand
indigenous
freshwater
species.
NZ
J.
Mar.
Freshwater
Res.
31:
185­
190.

Rosenfeld,
M.
1932.
Experimental
modification
of
mitosis
by
ammonia.
Arch.
Exp.
Zellforsch.
Gewebezuecht.
14:
1.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Rubin,
A.
J.
and
Elmaraghy,
G.
A.
1977.
Studies
on
the
toxicity
of
ammonia,
nitrate
and
their
mixtures
to
guppy
fry.
Water
Res.
11:
927­
935.

Ruyet,
J.
P.
and
Quemener,
H.
C.
1995.
Comparative
acute
ammonia
toxicity
in
marine
fish
and
plasma
ammonia
response.
Aquaculture
136:
181­
194.

Schaerdel,
A.
D.,
White,
W.
J.,
Lang,
C.
M.,
Dvorchik,
B.
H.,
and
Bohner,
K.
1983.
Localized
and
systemic
effects
of
environmental
ammonia
in
rats.
Lab.
Anim.
Sci.
33(
1):
40­
45.
117
Ammonia
(
CAS
No.
7664­
41­
7)
Schlesinger,
W.
H.
1991.
Biogeochemistry:
An
Analysis
of
Global
Change.
New
York:
Academic
Press.

Schubauer­
Berigan,
M.
K.,
Monson,
P.
D.,
West,
C.
W.,
and
Ankley,
G.
T.
1995.
Influence
of
pH
on
the
toxicity
of
ammonia
to
Chironomus
tentans
and
Lumbriculus
variegatus.
Environ.
Toxicol.
Chem.
14(
4):
713­
717.

Shah,
K.
D.
1988.
Hazardous
properties
of
ammonia,
Draft.
zitiert
nach:
DSM,
Inventory
of
data
on
chemicals,
Ammonia,
Delft.
1992.
RO471001/
4150J.
In
European
Commission.
1996.
Ammonia.
International
Uniform
Chemical
Information
Database.

Sheehan,
R.
J.
and
Lewis,
W.
M.
1986.
Influence
of
pH
and
ammonia
salts
on
ammonia
toxicity
and
water
balance
in
young
channel
catfish.
Trans.
Amer.
Fish.
Soc.
115:
891­
899.

Shimizu
H.
et
al.
1985.
Jap.
J.
Ind.
Health.
27:
400.
Silver,
S.
D.
and
McGrath,
F.
P.
1948.
A
comparison
of
acute
toxicities
of
ethylene
imine
and
ammonia
to
mice.
J.
Ind.
Hyg.
Toxicol.
30:
7­
9.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Silverman,
L.,
Whittenberger,
J.
L.,
and
Muller,
J.
1949.
Physiological
response
of
man
to
ammonia
in
low
concentrations.
J.
Ind.
Hyg.
Toxicol.
31:
74­
78.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Soderberg,
R.
W.
and
Meade,
J.
W.
1992.
Effects
of
sodium
and
calcium
on
acute
toxicity
of
unionized
ammonia
to
Atlantic
salmon
and
lake
trout.
J.
Appl.
Aquaculture
1(
4):
83­
92.

Stombaugh,
D.
P.,
Teague,
H.
S.,
and
Roller,
W.
L.
1969.
Effects
of
atmospheric
ammonia
on
the
pig.
J.
Anim.
Sci.
28(
6):
844­
847.

Tam,
N.
F.
Y.
and
Wong,
Y.
S.
1996.
Effect
of
ammonia
concentrations
on
growth
of
Chlorella
vulgaris
and
nitrogen
removal
from
media.
Bioresource
Technol.
57:
45­
50.

Thornton,
N.
C.
and
Setterstrom,
C.
1940.
Toxicity
of
ammonia,
chlorine,
hydrogen
cyanide,
hydrogen
sulphide,
and
sulphur,
dioxide
gases.
III.
Green
Plants.
Contrib.
Boyce
Thompson
Inst.
11:
343­
356.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Thurston,
R.
V.,
Russo,
R.
C.,
Luedtke,
R.
J.,
Smith,
C.
E.,
Meyn,
E.
L.,
Chakoumakos,
C.
and
Brown,
C.
J.
D.
1984.
Chronic
toxicity
of
ammonia
to
rainbow
trout.
Trans.
Amer.
Fish.
Soc.
113:
56­
73.

Thurston,
R.
V.,
Chakoumakos,
C.,
and
Russo,
R.
C.
1981.
Effect
of
fluctuating
exposures
on
the
acute
toxicity
of
ammonia
to
rainbow
trout
(
Salmo
gairdneri)
and
cutthroat
trout
(
S.
clarki).
Water
Res.
15:
911­
917.
118
Ammonia
(
CAS
No.
7664­
41­
7)
Thurston,
R.
V.
and
Russo,
R.
C.
1983.
Acute
toxicity
of
ammonia
to
rainbow
trout.
Trans.
Amer.
Fish.
Soc.
112:
696­
704.

Thurston,
R.
V.;
Russo,
R.
C.;
and
Phillips,
G.
R.
1983.
Acute
toxicity
of
ammonia
to
fathead
minnows.
Trans.
Amer.
Fish.
Soc.
112:
705­
711.

Thurston,
R.
V.,
Russo,
R.
C.
and
Smith,
C.
E.
1978.
Acute
toxicity
of
ammonia
and
nitrite
to
cutthroat
trout
fry.
Trans.
Amer.
Fish.
Soc.
107(
2):
361­
368.

Tomasso,
J.
R.
and
Carmichael,
G.
J.
1986.
Acute
toxicity
of
ammonia,
nitrite,
and
nitrate
to
the
Guadalupe
bass,
Micropterus
treculi.
Bull.
Environ.
Contam.
Toxicol.
36:
866­
870.

Tomasso,
J.
R.,
Goudie,
C.
A.,
Simco,
B.
A.,
and
Davis,
K.
B.
1980.
Effects
of
environmental
pH
and
calcium
on
ammonia
toxicity
in
channel
catfish.
Trans.
Amer.
Fish.
Soc.
109:
229­
234.

Toth,
B.
1972.
Hydrazine,
methylhydrazine
and
methylhydrazine
sulfate
carcinogenesis
in
Swiss
mice.
Failure
of
ammonium
hydroxide
to
interfere
in
the
development
of
tumors.
Int.
J.
Cancer.
9:
109.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Tsujii,
M.,
Kawano,
S.,
Tsuji,
S.,
Ito,
T.,
Nagano,
K.,
Sasaki,
Y.,
Hayashi,
N.,
Fusamoto,
H.,
and
Kamada,
T.
1993.
Cell
kinetics
of
mucosal
atrophy
in
rat
stomach
induced
by
long­
term
administration
of
ammonia.
Gastroenterol.
104:
796­
801.

Underhill,
F.
P.
and
Kapsinow,
R.
1922.
The
comparative
toxicity
of
ammonium
salts.
J.
Biol.
Chem.
54:
451­
457.

U.
S.
Environmental
Protection
Agency
(
USEPA).
1998a.
AP­
42.
Draft.
Vol.
1.
5th
ed.
Research
Triangle
Park:
Office
of
Air
Quality
Planning
and
Standards.

U.
S.
Environmental
Protection
Agency
(
USEPA).
1998b.
Emission
Factor
Documentation
for
AP­
42
Section
9.2.1.
Draft.
Research
Triangle
Park:
Office
of
Air
Quality
Planning
and
Standards.

U.
S.
Environmental
Protection
Agency
(
USEPA).
1998c.
1998
Update
of
Ambient
Water
Quality
Criteria
for
Ammonia.
Washington,
D.
C.:
Office
of
Water.
EPA
822­
R­
98­
008.

U.
S.
Environmental
Protection
Agency
(
USEPA).
1985.
Ambient
Water
Quality
Criteria
for
Ammonia.
1984.
Washington,
D.
C.:
Office
of
Water
Regulations
and
Standards,
Criteria
and
Standards
Division.
EPA­
50415­
85­
006.

U.
S.
Environmental
Protection
Agency
(
USEPA).
1999.
Development
of
Chemical
Categories
in
the
HPV
Challenge
Program.
www.
epa.
gov/
chemrtk/
catdoc29.
pdf.

Valentine,
H.
1964.
A
study
of
the
effect
of
different
ventilation
rates
on
the
ammonia
concentrations
in
the
atmosphere
of
broiler
houses.
Br.
Poult.
Sci.
5:
149­
159.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.
119
Ammonia
(
CAS
No.
7664­
41­
7)
Verberk,
M.
M.
1977.
Effects
of
ammonia
in
volunteers.
Arch.
Occup.
Environ.
Hlth.
39:
73­
81.

Verschueren,
K.
1996.
Handbook
of
Environmental
Data
and
Organic
Chemicals.
New
York:
John
Wiley
&
Sons,
Inc.

Vezina,
C.
1997.
National
Ammonia
Inventory.
Draft.
Ottawa:
Environment
Canada,
Pollution
Data
Brarnch.
In
Constable,
M.,
Jensen,
F.,
McLeron,
J.
Craig,
G.,
Moore,
D.
1999.
Canadian
Environmental
Protection
Act,
Priority
Substances
List
II:
Supporting
Document
for
Ammonia
in
the
Aquatic
Environment.
Draft,
Unpublished
Version.
Government
of
Canada,
Environment
Canada.

Vines,
H.
M.
and
Wedding,
R.
T.
1960.
Some
effects
of
ammonia
on
plant
metabolism
and
a
possible
mechanism
for
ammonia
toxicity.
Plant
Physiol.
35:
820­
825.

Wallingford,
G.
W.
1977.
Fate
of
nitrogen
from
fertilizer
practices.
In
Disposal
of
residues
on
land.
Rockville:
Information
Transfer,
Inc.
Pp.
152­
155.

Walsh,
L.
M.
1977.
Updating
the
nitrogen
cycle.
In
Disposal
of
residues
on
land.
Rockville:
Information
Transfer,
Inc.
Pp.
146­
151.

Warren,
K.
S.
1958.
The
differential
toxicity
of
ammonium
salts.
J.
Clin.
Invest.
37:
497­
501.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Warren
Spring
Laboratory.
1982.
Acidity
of
rainfall
in
the
United
Kingdom.

Weatherby,
J.
H.
1952.
Chronic
toxicity
of
ammonia
fumes
by
inhalation.
Proc.
Soc.
Exp.
Biol.
Med.
81:
300­
301.

Weiler,
R.
R.
1979.
Rate
of
loss
of
ammonia
from
water
to
the
atmosphere.
J.
Fish
Res.
Board
Can.
36:
685­
689.

Williams,
K.
A.,
Green,
D.
W.,
and
Pascoe,
D.
1986.
Studies
on
the
acute
toxicity
of
pollutants
to
freshwater
macroinvertebrates.
Arch.
Hydrobiol.
106(
1):
61­
70.

Wilson,
R.
P.,
Muhrer,
M.
E.,
and
Bloomfield,
R.
A.
1968.
Comparative
ammonia
toxicity.
Comp.
Biochem.
Physiol.
25:
295­
301.

Zimmerman,
P.
W.
1949.
Impurities
in
the
air
and
their
influence
on
plant
life.
In
Proceedings
of
the
First
National
Air
Pollution
Symposium.
Pasadean,
California.
Pp.
135­
141.
Zitiert
nach:
DSM,
Inventory
of
data
on
chemicals,
Ammonia,
Delft.
1992.
In
European
Commission.
1996.
Ammonia.
International
Uniform
Chemical
Information
Database.
In
World
Health
Organization
(
WHO).
1986.
Ammonia­
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.
120
Ammonia
(
CAS
No.
7664­
41­
7)
Zischke,
J.
A.
and
Arthur,
J.
W.
1987.
Effects
of
elevated
ammonia
levels
on
the
fingernail
clam,
Musculium
transversum,
in
outdoor
experimental
streams.
Arch.
Environ.
Contam.
Toxicol.
16:
225­
231.
APPENDIX
A
­
SIDS
DATA
AVAILABILITY
SUMMARY
Ammonia
(
CAS
No.
7664­
41­
7)
SIDS
DATA
AVAILABILITY
SUMMARY
DATE:
January
27,
2003
Ammonia
CAS
No:
7664­
41­
7
Information
OECD
Study
GLP
Other
Study
Estimated
Method
Acceptable
SIDS
Testing
Recommended
STUDY
Y/
N
Y/
N
Y/
N
Y/
N
Y/
N
Y/
N
Y/
N
PHYSICAL­
CHEMICAL
DATA
2.1
2.2
2.3
2.4
2.5
2.6
2.12
Melting
Point
Boiling
Point
Density
Vapour
Pressure
Partition
Coefficient
Water
Solubility
pH
and
pKa
Values
Oxidation:
Reduction
Potential
Y
Y
Y
Y
Y
Y
Y
Y
N
N
N
N
Y
N
N
N
N
N
N
N
Y
N
N
N
N
N
N
N
N
N
Y
N
N
N
N
N
N
N
N
N
Y
Y
Y
Y
Y
Y
Y
Y
N
N
N
N
N
N
N
N
OTHER
P/
C
STUDIES
RECEIVED
Y
N
N
Y
N
Y
N
ENVIRONMENTAL
FATE
and
PATHWAY
3.1.1
3.1.2
3.2
3.3
3.5
Photodegradation
Stability
in
Water
Monitoring
Data
Transport
and
Distribution
Biodegradation
Y
Y
Y
Y
Y
N
N
N
N
N
N
N
N
N
N
N
Y
N
Y
N
N
N
N
N
N
Y
Y
Y
Y
Y
N
N
N
N
N
OTHER
ENV
FATE
STUDIES
RECEIVED
Y
N
N
Y
N
Y
N
ECOTOXICITY
4.1
4.2
4.3
4.5.1
4.5.2
4.6.1
4.6.2
4.6.3
Acute
Toxicity
to
Fish
Acute
Toxicity
to
Daphnia
Toxicity
to
Algae
Chronic
Toxicity
to
Fish
Chronic
Toxicity
to
Daphnia
Toxicity
to
Soil
Dwelling
Organisms
Toxicity
to
Terrestrial
Plants
Toxicity
to
Birds
Y
Y
Y
Y
Y
N
Y
Y
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
Y
Y
Y
Y
Y
N
Y
Y
N
N
N
N
N
N
N
N
Y
Y
Y
Y
Y
­
Y
Y
N
N
N
N
N
N
N
N
OTHER
ECOTOXICITY
STUDIES
RECEIVED
Y
N
N
Y
N
Y
N
TOXICITY
5.1.1
5.1.2
5.1.3
5.4
5.5
5.6
5.8
5.9
5.11
Acute
Oral
Acute
Inhalation
Acute
Dermal
Repeated
Dose
Genetic
Toxicity
in
vitro
.
Gene
mutation
.
Chromosomal
aberration
Genetic
Toxicity
in
vivo
Reproduction
Toxicity
Development/
Teratogenicity
Human
Experience
N
Y
N
Y
Y
Y
Y
Y
N
Y
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
Y
N
Y
Y
Y
Y
Y
N
Y
N
N
N
N
N
N
N
N
N
N
­
Y
­
Y
Y
Y
Y
Y
­
Y
N
N
N
N
N
N
N
N
N
N
OTHER
TOXICITY
STUDIES
RECEIVED
Y
N
N
Y
N
Y
N
APPENDIX
B
­
COMPREHENSIVE
BIBLIOGRAPHY
COMPREHENSIVE
BIBLIOGRAPHY
Achorn,
F.
P.,
Balay,
H.
L.,
Myers,
E.
D.,
and
Grisso,
R.
D.
1971.
A
pollution
solution
for
granulation
plants.
Farm
Chem.
Croplife
134(
8):
28­
31.

Admiraal,
W.
1977.
Tolerance
of
estuarine
benthic
diatoms
to
high
concentrations
of
ammonia,
nitrite
ion,
nitrate
ion,
and
orthophosphate.
Mar.
Biol.
43:
307­
315.

Agency
for
Toxic
Substances
and
Disease
Registry
(
ATSDR).
1990.
Toxicological
Profile
for
Ammonia.

Aickin,
C.
C.,
Deisz,
R.
A.,
and
Lux,
H.
D.
1982.
Ammonium
action
on
post­
synaptic
inhibition
in
crayfish
neurons:
implications
for
the
mechanism
of
chloride
extrusion.
J.
Physiol.
329:
319­
339.

Al­
Dabbagh,
S.,
Forman,
D.,
Bryson,
D.,
Stratton,
I.,
and
Doll,
R.
1986.
Mortality
of
nitrate
fertiliser
workers.
British
J.
Ind.
Med.
43:
507­
515.

Alarie,
Y.
1981.
Dose­
response
analysis
in
animal
studies:
prediction
of
human
responses.
Env.
Health
Perspect.
42:
9­
13.

Alexander,
H.
C.,
Latvaitis,
B.,
and
Hopkins,
D.
L.
1986.
Site­
specific
toxicity
of
un­
ionized
ammonia
in
the
Tittabawassee
River
at
Midland,
Michigan:
Overview.
Environ.
Toxicol.
Chem.
5:
427­
435.

Alger,
B.
E.
and
Nicoll,
R.
A.
1983.
Ammonia
does
not
selectively
block
IPSPs
in
rat
hippocampal
pyramidal
cells.
J.
Neurophy.
49:
1381­
1391.

Allen,
G.
I.,
Eccles,
J.,
Nicoll,
R.
Z.,
Oshima,
T.,
and
Rubia,
F.
J.
1977.
The
ionic
mechanisms
concerned
in
generating
the
IPSPs
of
hippocampal
pyramidal
cells.
Roc.
R.
Soc.
Lond.
B.
198:
363­
384.

Alliance
Technologies
Corporation
for
the
U.
S.
EPA
1987.
Ammonia
Emission
Factors
for
the
NAPAP
(
National
Acid
Precipitation
Assessment
Program)
Emission
Inventory.
EPA/
600/
7­
87/
001.

Al­
Mashhadani,
E.
H.
and
Beck,
M.
M.
1985.
Effect
of
atmospheric
ammonia
on
the
surface
ultrastructure
of
the
lung
and
trachea
of
broiler
chicks.
Poul.
Sci.
64:
2056­
2061.

Alpatov,
I.
M.
1964.
A
study
of
gaseous
ammonia
toxicity.
Gig.
Tr.
Prof.
Zabol
2:
14­
18.
(
Russian).

Alpatov,
I.
M.
and
Mikhailov,
V.
I.
1963.
Inquires
into
gaseous
ammonia
toxicity
­
communications.
Gig.
Tr.
Prof.
Zabol
12:
51­
53.
(
Russian).

American
Conference
of
Governmental
Industrial
Hygienists,
Inc.
(
ACGIH).
1986.
Documentation
of
the
Threshold
Limit
Values
and
Biological
Exposure
Indices.
5th
ed.
Cincinnati:
ACGIH.
Pp.
27.
American
Society
of
Testing
and
Materials
(
ASTM).
1984.
Standard
test
method
for
estimating
sensory
irritancy
of
airborne
chemicals.
E
981­
984.
Ames,
R.
G.
1991.
Multiple­
episode
conjunctivitis
outbreak
among
workers
at
a
nut
processing
facility.
J.
Occup.
Med.
34:
505­
509.

Anderson,
D.
P.,
Beard,
C.
W.,
and
Hanson,
R.
P.
1964.
The
adverse
effects
of
ammonia
on
chickens
including
resistance
to
infection
with
newcastle
disease
virus.
Avian
Dis.
8:
369­
379.

Anghileri,
L.
J.
1975.
Tumor
growth
inhibition
by
ammonium
chloride­
induced
acidosis.
Int.
J.
Pharmacol.
12:
320­
326.

Ankley,
G.
T.,
Schubauer­
Berigan,
and
Monson,
P.
D.
1995.
Influence
of
pH
and
hardness
on
toxicity
of
ammonia
to
the
amphipod
Hyalella
azteca.
Can.
J.
Fish.
Aquat.
Sci.
52:
2078­
2083.

Anonymous.
1988a.
Ammonia
makers
are
bullish
in
'
88.
Chem.
Mktg.
Rep.
233:
3­
28.

Anonymous.
1988b.
Ammonia
runs
full,
but
prices
in
question.
Chem.
Mktg.
Rep.
234:
3­
32.

Anonymous.
1988c.
Inorganics:
Despite
1987'
s
growth,
output
of
many
products
was
below
levels
of
late
1970'
s.
Chem.
Eng.
News
June.
20:
41.

Anonymous.
1988d.
Nitrogen:
Output
edged
higher
but
remained
well
below
recent
peak.
Chem.
Eng.
News
June.
20:
43.

Anonymous.
1988e.
Output
will
be
up
this
year
and
next
for
nearly
all
major
chemicals
and
polymers:
inorganic
chemicals.
Chem.
Eng.
News
Dec.
12:
30.

Anonymous.
1988f.
World:
Chemical
and
polymer
production
growth,
1986­
2000.
Chem.
Mktg.
Rep.
Pp.
14.

Anonymous.
1959.
The
Chemistry
of
Industrial
Toxicology.
In
Elkins,
H.
B.
(
ed.).
New
York:
J.
Wiley
&
Sons,
Inc.

Anthonisen,
A.
C.,
Loehr,
R.
C.,
Prakasam,
T.
B.
S.,
and
Srinath,
E.
G.
1976.
Inhibition
of
nitrification
by
ammonia
and
nitrous
acid.
J.
Water
Poll.
Control
Fed.
48:
835­
852.

Appelman,
L.
M.,
ten
Berge,
W.
F.,
and
Reuzel,
P.
G.
J.
1982.
Acute
inhalation
toxicity
study
of
ammonia
in
rats
with
variable
exposure
periods.
Am.
Ind.
Hyg.
Assoc.
J.
43:
662­
665.

Arillo,
A.,
Margiocco,
C.,
Melodia,
F.,
Mensi,
P.,
and
Schenone,
G.
1981.
Ammonia
toxicity
mechanism
in
fish:
studies
on
rainbow
trout
(
Salmo
gairdneri
Rich.).
Ecotoxicol.
Environ.
Safety
5:
316­
328.
Armstrong,
D.
A.,
Chippendale,
D.,
Knight,
A.
W.,
and
Colt,
J.
E.
1978.
Interaction
of
ionized
and
un­
ionized
ammonia
on
short­
term
survival
and
growth
of
prawn
larvae,
Macrobrachium
rosenbergii.
Biol.
Bull.
154:
15­
31.

Arthur,
J.
W.,
West,
C.
W.,
Allen,
K.
N.,
and
Hedtke,
S.
F.
1987.
Seasonal
toxicity
of
ammonia
to
five
fish
and
nine
invertebrate
species.
Environ.
Contam.
Toxicol.
38:
324­
331.

Asman,
W.
A.
and
Janssen,
A.
J.
1987.
A
long­
range
transport
model
for
ammonia
and
ammonium
for
Europe.
Atmos.
Environ.
21(
10).
In
Constable,
M.,
Jensen,
F.,
McLernon,
J.
Craig,
G.,
Moore,
D.
1999.
Canadian
Environmental
Protection
Act,
Priority
Substances
List
II:
Supporting
document
for
Ammonia
in
the
Aquatic
Environment.
Draft,
Unpublished
Version.
Government
of
Canada,
Environment
Canada
Austin,
E.
R.
and
Lee,
G.
F.
1973.
Nitrogen
release
from
lake
sediments.
J.
Water
Pollut.
Control
Fed.
45:
870­
879.

Bacon,
C.
W.
1986.
Effects
of
broiler
litter
volatiles
and
ammonia
on
fungal
spore
germination.
Poul.
Sci.
65:
710­
716.

Bader,
J.
A.
and
Grizzle,
J.
M.
1992.
Effects
of
ammonia
on
growth
and
survival
of
recently
hatched
channel
catfish.
Amer.
Fish.
Soc.
4:
17­
23.

Baker,
J.
H.
et
al.
1959.
Determination
of
application
losses
of
anhydrous
ammonia.
Agronomy
Journal
51:
361­
362.
In
U.
S.
Environmental
Protection
Agency
(
USEPA).
1998b.
Emission
Factor
Documentation
for
AP­
42
Section
9.2.1.
Draft.
Research
Triangle
Park:
Office
of
Air
Quality
and
Standards.

Barrow,
C.
S.,
Alarie,
Y.,
and
Stock,
M.
F.
1978.
Sensory
irritation
and
incapacitation
evoked
by
thermal
decomposition
products
of
polymers
and
comparisons
with
known
sensory
irritants.
Arch.
Environ.
Health
33:
79­
88.

Barrow,
C.
S.,
and
Steinhagen,
W.
H.
1980.
NH3
concentrations
in
the
expired
air
of
the
rat:
Importance
to
inhalation
toxicology.
Toxicol.
Appl.
Pharmacol.
53:
116­
121.

Barton,
L.
V.
1940.
Toxicity
of
ammonia,
chlorine,
hydrogen
cyanide,
hydrogen
sulphide,
and
sulphur
dioxide
gases.
IV.
Seeds.
Contrib.
Boyce
Thompson
Inst.
11:
357­
363.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Barzel,
U.
S.
1975.
The
effect
of
chronic
ammonium
chloride
ingestion
on
parathyroid
hormone
function.
Nephron.
14:
339­
346.

Barzel,
U.
S.
and
Jowsay,
J.
1969.
The
effects
of
chronic
acid
and
alkali
administration
on
bone
turnover
in
adult
rats.
Clin.
Sci.
36:
517­
524.

BASF
AG,
Sicherheitsdatenblatt
Ammoniak
fluessig
(
27.04.94).
In
European
Commission.
1996.
Ammonia.
International
Uniform
Chemical
Information
Database.
BASF
AG,
Abteilung
Analytik;
unveroeffentlichte
Untersuchung
(
BRU
92.004).
In
European
Commission.
1996.
Ammonia.
International
Uniform
Chemical
Information
Database.

Batshaw,
M.
L.
and
Monahan,
P.
S.
1987.
Treatment
of
Urea
Cycle
Disorders.
Recent
Adv.
Inborn
Errors
of
Metabolism
Proc.
4th
Int.
Congr.,
Sendai
Enzyme
38:
242­
250.

Bauer,
C.
F.
1985.
Emissions
of
vapor­
phase
fluorine
and
ammonia
from
the
Columbia
coalfired
power
plant.
Environ.
Sci.
Tech.
19:
1099­
1103.

Beaumont,
A.
R.
and
Budd,
M.
D.
1984.
Marine.
Poll.
Bull.
15:
402­
405.
In
Vorlaeufige
Ergebnisse
des
F+
E­
Vorhabens
Bewertung
wassergefaehrdender
Stoffe.
1985.
vgl.
Datenblaetter
zum
Katalog
wassergefaehrdender
Stoffee,
KBwS,
Nr.
5.
In
European
Commission.
1996.
Ammonia.
International
Uniform
Chemical
Information
Database.

Benedict,
H.
M.
and
Breen,
W.
H.
1955.
The
use
of
weeds
as
a
means
of
evaluating
vegetation
damage
caused
by
air
pollution.
Proc.
3rd
Natl.
Pollution
Symp.
Pasadena,
Calif.
Pp.
177­
190.
Zitiert
nach:
DSM,
Inventory
of
data
on
chemicals,
Ammonia,
Delft,
1992.
RO471001/
4150J.
In
European
Commission.
1996.
Ammonia.
International
Uniform
Chemical
Information
Database.

Benjamin,
A.
M.
1981.
Control
of
glutaminase
activity
in
rat
brain
cortex
in
vitro:
Influence
of
glutamate,
phosphate,
ammonium,
calcium
and
hydrogen
ions.
Brain
Res.
208:
363­
377.

Benyajati,
S.
and
Goldstein,
L.
1975.
Renal
glutaminase
adaptation
and
ammonia
excretion
in
infant
rats.
Am.
J.
Physiol.
228:
693­
698.

Bergerhouse,
D.
L.
1993.
Lethal
effects
of
elevated
pH
and
ammonia
on
early
life
stages
of
hybrid
striped
bass.
In:
Tave,
D.
(
ed.).
Strategies
and
Tactics
for
Management
of
Fertilized
Hatchery
Ponds.
New
York:
The
Haworth
Press,
Inc.
Pp.
81­
101.

Bergerhouse,
D.
L.
1992.
Lethal
effects
of
elevated
pH
and
ammonia
on
early
life
stages
of
walleye.
N.
Amer.
J.
Fish.
Man.
12:
356­
366.

Beri,
S.,
Takagaki,
G.,
Clarke,
D.
D.,
and
Waelsch,
H.
1962.
Metabolic
compartments
in
vivoammonia
and
glutamic
acid
metabolism
in
brain
and
liver.
J.
Biol.
Chem.
237:
2562­
2569.

Besser,
J.
M.,
Ingersoll,
C.
G.,
Leonard,
E.
N.,
Mount,
D.
R.
1988.
Effect
of
zeolite
on
toxicity
of
ammonia
in
freshwater
sediments:
implications
for
toxicity
identification
evaluation
procedures.
Environ.
Toxicol.
Chem.
17(
11):
2310­
2317.

Bessman,
S.
P.
and
Pal,
N.
1982.
Ammonia
intoxication:
Energy
metabolism
and
brain
protein
synthesis.
Isr.
J.
Med.
Sci.
18:
171­
175.

Bessman,
S.
P.
and
Bessman,
A.
N.
1955.
The
cerebral
and
peripheral
uptake
of
ammonia
in
liver
disease
with
a
hypothesis
for
the
mechanism
of
hepatic
coma.
J.
Clin.
Invest.
34:
622­
628.
Betterton,
E.
A.
1992.
In
Nriagu,
J.
O.
(
ed.).
Gaseous
Pollutants:
Characterization
and
Cycling.
New
York:
John
Wiley
&
Sons,
Inc.
Pp.
1­
50.

Beyenbach,
K.
W.
1985.
Comparative
physiology
of
the
renal
proximal
tubule.
Renal
Physiol.
8:
222­
236.

Bhattacharya,
S.
and
Mukherjee,
S.
1976.
Activity
of
the
hepatopancreatic
protease
and
esterase
in
fish
exposed
t
industrial
pollutants.
Comp.
Physiol.
Ecol.
1:
45­
50.

BIBRA.
1995.
Toxicology
Profile:
Ammonia.
BIBRA
International.

Bimbaum,
S.
M.,
Winitz,
M.,
and
Greenstein,
J.
P.
1957.
Quantitative
nutritional
studies
with
water­
soluable
chemically
defined
diets.
III.
Individual
amino
acids
as
sources
of
"
nonessential
nitrogen.
Arch.
Biochem.
Biophys.
72:
428­
436.
Bittershol,
G.
1971.
Epidemiologic
study
on
cancer
of
workers
in
a
chemical
plant.
Arch.
Gesh.
38:
198­
209.
(
Original
in
German).

Blade,
M.
C.,
Arola,
L.
and
Alemany,
M.
1988.
Rapid
detoxification
of
infused
ammonium
by
the
anesthetized
rat.
Biochem.
Internatl.
16:
859­
867.

Blalock,
J.
E.
1989.
A
molecular
basis
for
bidirectional
communication
between
the
immune
and
neuroendocrine
systems.
Physiol.
Rev.
69:
1­
32.

Bloomfield,
R.
A.,
Kearley,
E.
O.,
Creach,
D.
O.,
and
Muhrer,
M.
E.
1963.
Ruminal
pH
and
absorption
of
ammonia
and
VFA.
J.
Anim.
Sci.
22(
1):
833.

Bolon,
B.,
Bonnefoi,
M.
S.,
Roberts,
K.
C.,
Marshall,
M.
W.,
and
Morgan,
K.
T.
1991.
Toxic
interactions
in
the
rat
nose:
pollutants
from
soiled
bedding
and
methyl
bromide.
Toxicological
Pathol.
19(
4
pt.
2):
571­
579.

Bond,
G.
G.,
Shellenberger,
R.
F.,
Flores,
G.
H.,
Cook,
R.
R.,
and
Fishbeck,
W.
A.
1985.
A
case
control
study
of
renal
cancer
mortality
at
a
Texas
chemical
plant.
Am.
J.
Ind.
Med.
7:
123­
140.

Booth,
N.
H.
and
McDonald,
L.
E.
1982.
Veterinary
Pharmacology
and
Therapeutics.
5th
edition.
Pp.
938­
939.

Bouhuys,
A.
1977.
Environmental
lung
diseases.
The
Physiology
of
Breathing.

Boutros,
A.
R.,
Esfandiari,
S.,
Orlowski,
J.
P.,
and
Smith,
J.
S.
1980.
Reye
syndrome:
A
predictably
curable
disease.
Pediatr.
Clin.
North
Am.
27:
539­
552.

Bower,
C.
E.
and
Bidwell,
J.
P.
1978.
Ionization
of
ammonia
in
seawater:
effects
of
temperature,
pH,
and
salinity.
J.
Fish.
Res.
Board
Can.
35:
1012­
1016.

Boyd,
E.
M.,
MacLachlan,
M.
L.,
and
Perry,
W.
F.
1944.
Experimental
ammonia
gas
poisoning
in
rabbits
and
cats.
J.
Ind.
Hyg.
Toxicol.
26:
29­
34.
Boyd,
E.
M.
and
Seymour,
K.
G.
W.
1946.
Ethylenediamine
dihydrochloride
or
"
chlor­
ethamine".
Exp.
Med.
Surg.
4:
223­
227.

Bradford,
H.
F.
and
Ward,
H.
K.
1975.
Glutamine
as
a
metabolic
substrate
for
isolated
nerveendings
Inhibition
by
ammonium
ions.
Biochem.
Soc.
Trans.
­
559th
Meeting.
3:
1223­
1226.

Braun,
E.
J.
1985.
Comparative
aspects
of
the
urinary
concentrating
process.
Renal
Physiol.
8:
249­
260.

Braunwald,
E.,
Isselbacher,
K.
J.,
Petersdori,
R.
G.,
Wilson,
J.
D.,
Martin,
J.
B.,
and
Fauci,
A.
S.
1987.
Harrison's
Principles
of
Internal
Medicine.
McGraw­
Hill
Publ.
Pp.
1054.

Bressani,
R.
and
Brahma,
J.
E.
1977.
Effect
of
frequency
of
feeding
on
blood
serum
urea
changes
in
dogs.
Nutr.
Rep.
Int.
16:
305­
316.

Brock,
T.
D.
1970.
Biology
of
Microorganisms.
Englewood
Cliffs,
NJ.:
Prentice
Hall,
Inc.
Broderius,
S.,
Drummond,
R.,
Fiandt,
J.,
and
Russom,
C.
1985.
Toxicity
of
ammonia
to
early
life
stages
of
the
smallmouth
bass
at
four
pH
values.
Environ.
Toxicol.
Chem.
4:
87­
96.

Broderson,
J.
R.,
Lindsey,
J.
R.,
and
Crawford,
J.
E.
1976.
The
role
of
environmental
ammonia
in
respiratory
mycoplasmosis
of
rats.
Am.
J.
Pathol.
85:
115­
130.
In
World
Health
Organization
(
WHO).
1986.
Ammonia­
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Brown,
D.
A.
and
McLeay,
D.
J.
1975.
Effect
of
nitrite
on
methemoglobin
and
total
hemoglobin
of
juvenile
rainbow
trout.
Prog.
Fish­
Cult.
37(
1):
36­
38.

Brown,
V.
M.
1968.
The
calculation
of
the
acute
toxicity
of
mixtures
of
poisons
to
rainbow
trout.
Water
Res.
2:
723­
733.

Brown,
V.
M.,
Jordan,
D.
H.
M.,
and
Tiller,
B.
A.
1969.
The
acute
toxicity
to
rainbow
trout
of
fluctuating
concentrations
and
mixtures
of
ammonia,
phenol
and
zinc.
J.
Fish.
Biol.
1:
1­
9.

Buckley,
J.
A.
1978.
Acute
toxicity
of
un­
ionized
ammonia
to
fingerling
coho
salmon.
Prog.
Fish­
Cult.
40(
1):
30­
32.

Buckley,
L.
A.,
Jiang,
X.
Z.,
James,
R.
A.,
Morgan,
K.
T.,
and
Barrow,
C.
S.
1984.
Respiratory
tract
lesions
induced
by
sensory
irritants
at
the
RD50
concentration.
Toxicol.
Appl.
Pharmacol.
74:
417­
429.

Budavari,
S.
(
ed.).
1996.
Merck
Index.
12th
ed.
Whitehouse
Station:
Merck
Research
Laboratories.

Burkhalter,
D.
E.
and
Kaya,
C.
M.
1977.
Effects
of
prolonged
exposure
to
ammonia
on
fertilized
eggs
and
sac
fry
of
rainbow
trout
(
Salmo
gairdneri).
Trans.
Amer.
Fish
Soc.
106(
5):
470­
474.
Cadle,
S.
H.
1985.
Seasonal
variations
in
nitric
acid,
nitrate,
strong
aerosol
acidity,
and
ammonia
in
an
urban
area.
Atmos.
Environ.
19:
181­
188.

Cadle,
S.
H.,
Countess,
R.
J.,
and
Kelly,
N.
A.
1982.
Nitric
acid
and
ammonia
in
urban
and
rural
locations.
Atmos.
Environ.
16:
2501­
2506.

Calamari,
D.,
Marchetti,
R.,
and
Vailati,
G.
1981.
Effects
of
long­
term
exposure
to
ammonia
on
the
developmental
stages
of
rainbow
trout
(
Salmo
gairdneri
Richardson).
Rapp.
P.­
v.
Reun.
Cons.
int.
Explor.
Mer
178:
81­
86.

Cameron,
J.
N.
and
Heisler,
N.
1983.
Studies
of
ammonia
in
the
rainbow
trout:
physicochemical
parameters,
acid­
base
behaviour
and
respiratory
clearance.
J.
Exp.
Biol.
105:
107­
125.

Canadian
Fertilizer
Institute
(
CFI).
1996.
1995
Emissions
inventory,
update.
Ottawa.
In
Constable,
M.,
Jensen,
F.,
McLeron,
J.
Craig,
G.,
Moore
D.
1996.
Canadian
Environmental
Protection
Act,
Priority
Substances
List
II:
Supporting
Document
for
Ammonia
in
the
Aquatic
Environment.
Draft,
Unpublished
Version.
Government
of
Canada,
environment
Canada.

Caplin,
M.
1941.
Ammonia­
gas
poisoning:
Forty­
seven
cases
in
a
London
shelter.
Lancet
Pp.
95­
97.

Challoner,
K.
R.
and
McCarron,
M.
M.
1988.
Ammonium
nitrate
cold
pack
ingestion.
J.
Emergency
Med.
6:
289­
293.

Charles,
D.
R.
and
Payne,
C.
F.
1966.
The
influence
of
graded
levels
of
atmospheric
ammonia
on
chickens.
I.
Effects
on
respiration
and
on
the
performance
of
broilers
and
replacement
growing
stock.
Br.
Poult.
Sci.
7:
177­
187.

Charles,
D.
R.
and
Payne,
C.
G.
1966.
The
influence
of
graded
levies
of
atmospheric
ammonia
on
chickens.
II.
Effects
on
the
performance
of
laying
hens.
Br.
Poult.
Sci.
7:
189­
198.

Chassin,
J.
L.
and
Slattery,
L.
R.
1953.
Jejungal
structure
due
to
ingestion
of
ammonia:
Report
of
a
case
complicated
by
severe
potassium
deficiency.
J.
Am.
Med.
Assoc.
152:
134­
136.

1996.
Chem.
Eng.
News.
74:
13.
In
Hazardous
Substances
Database
(
HSDB).
1999.
Ammonia.
National
Library
of
Medicine,
Bethesda,
MD.

1980.
C
and
EN's
top
fifty
chemical
products.
Chem.
Eng.
News.
16.
In
World
Health
Organization
(
WHO).
1986.
Ammonia­
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Chen,
J.
C.
and
Kou,
Y.
Z.
1993.
Accumulation
of
ammonia
in
the
haemolymph
of
Penaeus
monodon
exposed
to
ambient
ammonia.
Aquaculture
109:
177­
185.
Clawson,
A.
J.
and
Amstrong,
W.
D.
1981.
Ammonium
polyphosphate
as
a
source
of
phosphorus
and
nonprotein
nitrogen
for
monogastrics.
J.
Anim.
Sci.
52:
1­
7.

Clement
Associates
Inc.
1990.
Health
Effects
Assessment
for
Ammonia.
The
Fertilizer
Institute:
Washington,
D.
C.

Close,
L.
G.,
Catlin,
F.
I.,
and
Cohn,
A.
M.
1980.
Acute
and
chronic
effects
of
ammonia
burns
of
the
respiratory
tract.
Arch.
Otolaryngol.
106:
151­
158.

Coffee,
R.
C.
and
Bartholomew,
W.
V.
1964.
Some
aspects
of
ammonia
sorption
by
soil
surfaces.
Soil
Sci.
Soc.
Proc.
28(
4):
485­
490.

Cole,
T.
J.,
Cotes,
J.
E.,
Johnson,
G.
R.,
de
V.
Martin,
H.,
Reed,
J.
W.
and
Saunders,
M.
J.
1977.
Ventilation,
cardiac
frequency
and
pattern
of
breathing
during
exercise
in
men
exposed
to
o­
chlorobenzylidene
malononitrile
(
CS)
and
ammonia
gas
in
low
concentrations.
Quart.
J.
Exper.
Physiol.
62:
341­
351.

Colt,
J.
and
Tchobanoglous,
G.
1978.
Chronic
exposure
of
channel
catfish
Ictalurus
punctatus,
to
ammonia:
effects
on
growth
and
survival.
Aquaculture
15:
353­
372.

Cometto­
Muniz,
J.
E.
and
Hernandez,
S.
M.
1990.
Odorous
and
pungent
attributes
of
mixed
and
unmixed
odorants.
Perception
and
Psychophysics
47(
4):
391­
399.

Compressed
Gas
Association,
Inc.
1990.
Handbook
of
Compressed
Gases.
3rd
ed.
Pp.
259­
260.
In
Compressed
Gas
Association,
Inc.
1999.
American
National
Standard
Safety
Requirements
for
the
Storage
and
Handling
of
Anhydrous
Ammonia.
5th
ed.
Arlington:
Compressed
Gas
Association,
Inc.

Conn,
H.
E.
1972.
Studies
of
the
source
and
significance
of
blood
ammonia
IV.
Early
ammonia
peaks
after
ingestion
of
ammonium
salts.
Yale
J.
Biol.
Med.
45:
543­
549.

Constable,
M.,
Jensen,
F.,
McLeron,
J.
Craig.,
G.,
Moore,
D.
1999.
Canadian
Environmental
Protection
Act,
Priority
Substances
List
II:
Supporting
Document
for
Ammonia
in
the
Aquatic
Environment.
Draft,
Unpublished
Version.
Government
of
Canada,
Environment
Canada.

Coon,
R.
A.,
Jones,
R.
A.,
Jenkins,
Jr,
L.
J.,
and
Siegel,
J.
1970.
Animal
inhalation
studies
on
ammonia,
ethylene,
glycol,
formaldehyde,
dimethylamine,
and
ethanol.
Toxicol.
Appl.
Pharmacol.
16:
646­
655.

Corpron,
K.
E.
and
Armstrong,
D.
A.
1983.
Removal
of
nitrogen
by
an
aquatic
plant,
elodea
densa,
in
recirculating
macrobrachium
culture
systems.
Aquaculture
32:
347­
360.

Cralley,
L.
V.
1942.
The
effect
of
irritant
gases
upon
the
rate
of
ciliary
activity.
J.
Ind.
Hyg.
Tox.
24:
193­
198.
Cribb,
G.
S.
1964.
Nitrogen.
Mellor's
Comprehensive
Treatise
on
Inorganic
and
Theoretical
Chemistry.
London:
Longmans,
276­
327.

Crook,
B.,
Robertson,
J.
F.,
Travers
Glass,
S.
Z.,
Botheroyd,
E.
M.,
Lacey,
J.,
and
Topping,
M.
D.
1991.
Airborne
dust,
ammonia,
microorganisms,
and
antigens
in
pig
confinement
houses
and
the
respiratory
health
of
exposed
farm
workers.
Am.
Ind.
Hyg.
Assoc.
J.
52(
7):
271­
279.

Curtis,
S.
E.,
Anderson,
C.
R.,
Simon,
J.,
Jensen,
A.
J.,
Day,
D.
L.,
and
Kelley,
K.
W.
1975.
Effects
of
aerial
ammonia,
hydrogen
sulfide
and
swine­
house
dust
on
rate
of
gain
and
respiratorytract
structure
in
swine.
J.
Anim.
Sci.
41:
735­
739.

Dabrowski,
H.
and
Sikora,
H.
1986.
Acute
toxicity
of
ammonia
to
common
carp
(
Cyprinus
carpio
L.).
Pol.
Arch.
Hydrobiol.
33(
1):
121­
128.

Dalhamn,
T.
1963.
Effect
of
ammonia
alone
and
combined
with
carbon
particles
on
ciliary
activity
in
the
rabbit
trachea
in
vivo,
with
studies
of
the
absorption
capacity
of
the
nasal
cavity.
Int.
J.
Air.
Wat.
Poll.
7:
531­
539.

Dalhmanm,
T.
1963.
Mucous
flow
and
ciliary
activity
in
the
trachea
of
healthy
rats
exposed
to
respiratory
irritant
gases
(
SO2,
H3N,
HCHOO).
VIII.
The
reaction
of
the
tracheal
ciliary
activity
to
single
exposure
to
respiratory
irritant
gases
and
studies
of
the
pH.
Acta.
Physiol.
Scand.
123S:
93­
105.

Dalhamn,
T.
1956.
The
reaction
of
the
tracheal
ciliary
activity
to
single
exposure
to
respiratory
irritant
gases
and
studies
of
the
pH.
Acta.
Physiol.
Scand.
36S:
93­
105.

Dalhamn,
T.
and
Reid,
L.
Ciliary
Activity
and
Histologic
Observations
in
the
Trachea
After
Exposure
to
Ammonia
and
Carbon
Particles.
In
Davis,
C.
N.
(
ed.).
Inhaled
Particles
and
Vapors.
II.
Proceedings
of
an
International
Symposium.
New
York:
Pergamon
Press.
Pp.
299­
306.

Dalhamn,
T.
and
Sjoholm,
J.
1963.
Studies
on
SO2,
NO2,
and
NH3:
Effect
on
ciliary
activity
in
rabbit
trachea
of
single
in
vitro
exposure
and
resorption
in
rabbit
nasal
cavity.
Acta.
Physiol.
Scand.
58:
287­
291.

Daoust,
P.
Y.
and
Ferguson,
H.
W.
1984.
The
pathology
of
chronic
ammonia
toxicity
in
rainbow
trout,
Salmo
gairdneri
Richardson.
J.
Fish
Dis.
7:
199­
205.

Day,
D.
L.,
Hansen,
E.
L,
and
Anderson,
S.
1965.
Gases
and
odors
in
confinement
swine
buildings.
Trans.
Am.
Soc.
Agric.
Eng.
8:
118­
121.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

De,
S.
N.
and
Bhattacharya,
S.
1976.
Effect
of
some
industrial
pollutants
on
fish
thyroid
peroxidase
activity
and
role
of
cytochrome
c
thereon.
Indian
J.
Exp.
Biol.
14:
561­
563.

Deaton,
J.
W.,
Reece,
F.
N.,
and
Lott,
B.
D.
1984.
Effect
of
atmospheric
ammonia
on
pullets
at
point
of
lay.
Poult.
Sci.
63:
384­
385.
Deaton,
J.
W.,
Reece,
F.
N.,
and
Lott,
B.
D.
1982.
Effect
of
atmospheric
ammonia
on
laying
hen
performance.
Poult.
Sci.
61:
1815­
1817.

Deaton,
J.
W.,
Reece,
F.
N.,
and
Thornberry,
F.
D.
1986.
Atmospheric
ammonia
and
incidence
of
blood
spots
in
eggs.
Poult.
Sci.
65:
1427­
1428.

Demerec,
M.,
Bertani,
G.,
and
Flint,
J.
1951.
A
survey
of
chemicals
for
mutagenic
action
on
E.
coli.
Amer.
Natural.
85:
119­
136.

Denmead,
O.
T.
et
al.
1982.
Atmospheric
dispersion
of
ammonia
during
application
of
anhydrous
ammonia
fertilizer.
J.
Environ.
Qual.
11(
4):
568­
572.
In
U.
S.
Environmental
Protection
Agency
(
USEPA).
1998a.
AP­
42.
Draft.
Vol.
1.
5th
ed.
Research
Triangle
Park:
Office
of
Air
Quality
Planning
and
Standards.

Denmead,
O.
T.
et
al.
1977.
A
direct
field
measurement
of
ammonia
emission
after
injection
of
anhydrous
ammonia.
Soil
Sci.
Soc.
Amer.
J.
41:
1001­
1004.
In
U.
S.
Environmental
Protection
Agency
(
USEPA).
1998a.
AP­
42.
Draft.
Vol.
1.
5th
ed.
Research
Triangle
Park:
Office
of
Air
Quality
Planning
and
Standards.

Diamond,
J.
M.,
Mackler,
D.
G.,
Rasnake,
W.
J.,
and
Gruber,
D.
1993.
Derivation
of
site­
specific
ammonia
criteria
for
an
effluent­
dominated
headwater
stream.
Environ.
Toxicol.
Chem.
12:
649­
658.

Diekman,
M.
A.,
Scheidt
A.
B.,
Sutton,
A.
L.,
Green,
M.
L.,
Clapper,
J.
A.,
Kelly,
D.
T.,
and
Van
Alstine,
W.
G.
1993.
Growth
and
reproductive
performance,
during
exposure
to
ammonia,
of
gilts
afflicted
with
pneumonia
and
atrophic
rhinitis.
Am.
J.
Vet.
Res.
54(
12):
2128­
2131.

Directive
92/
32
of
April
30,
1992.
1992.
Official
Journal
of
the
European
Communities,
No.
L154
of
June
5,
1992.

Dodd,
K.
T.
and
Gross,
D.
R.
1980.
Ammonia
inhalation
toxicity
in
cats:
A
study
of
acute
and
chronic
respiratory
dysfunction.
Arch.
Environ.
Health
35:
6­
14.

Doig,
P.
A.
and
Willoughby,
R.
A.
1971.
Response
of
swine
to
atmospheric
ammonia
and
organic
dust.
J.
Am.
Vet.
Med.
Assoc.
159:
1353­
1361.

Donham,
K.
J.
and
Popendorf,
W.
J.
1985.
Ambient
levels
of
selected
gases
inside
swine
confinement
buildings.
Am.
Hyg.
Assoc.
J.
46:
658­
661.

Donham,
K.
J.
and
Zavala,
D.
C.
1984.
Acute
effects
of
work
environment
on
pulmonary
function
of
swine
confinement
workers.
Am.
J.
Ind.
Med.
5:
367­
375.

Douglas,
R.
B.
and
Coe,
J.
E.
1987.
The
relative
sensitivity
of
the
human
eye
and
lung
to
irritant
gases.
Ann.
Occup.
Hyg.
31:
265­
267.

Doyle,
G.
J.,
Tuazon,
E.
C.,
Graham,
R.
A.,
Mischke,
T.
M.,
Winer,
A.
M.,
and
Pitts,
Jr.,
J.
N.
1979.
Simultaneous
concentrations
of
ammonia
and
nitric
acid
in
a
polluted
atmosphere
and
their
equilibrium
relationship
to
particulate
ammonium
nitrate.
Environ.
Sci.
Tech.
13(
11):
1416­
1419.
Drummond,
J.
G.,
Curtis,
S.
E.,
Simon,
J.,
and
Norton,
H.
W.
1980.
Effects
of
aerial
ammonia
on
growth
and
health
of
young
pigs.
J.
Anim.
Sci.
50(
6):
1085­
1091.

DSM,
Inventory
of
Data
on
chemicals,
Ammonia,
Delft.
1992.
LRO471001/
4150J.
In
European
Commission.
1996.
Ammonia.
International
Uniform
Chemical
Information
Database.

Duda,
G.
D.
and
Handler,
P.
1958.
Kinetics
of
ammonia
metabolism
in
vivo.
J.
Biol.
Chem.
232:
303­
314.

du
Ruisseau,
J.
P.,
Greenstein,
J.
P.,
Winitz,
M.,
and
Birnbaum,
S.
M.
1957.
Studies
on
the
metabolism
of
amino
acids
and
related
compounds
in
vivo.
VI.
Free
amino
acid
levels
in
the
tissues
of
rats
protected
against
ammonia
toxicity.
Arch.
Biochem.
Biophysiol.
68:
161­
171.

Ecological
Analysts,
Inc.
1981.
The
Sources,
Chemistry,
Fate,
and
Effects
of
Ammonia
in
Aquatic
Environments.
Washington,
DC:
American
Petroleum
Institute.

Egle,
Jr.,
J.
L.
1973.
Retention
of
inhaled
acetone
and
ammonia
in
the
dog.
Am.
Ind.
Hyg.
Assoc.
J.
34:
533­
539.

Ehrlich,
R.
1966.
Effect
of
nitrogen
dioxide
on
resistance
to
respiratory
infection.
Bact.
Rev.
30:
604­
614.

Elissa,
M.
G.
1983.
Arch.
Hydrobiol.
98(
3):
327­
343.

Ellen,
G.
and
Schuller.
1982.
No
volatile
N­
nitrosamines
detected
in
blood
and
urine
from
patients
ingesting
daily
large
amounts
of
ammonium
nitrate.
Fd.
Chem.
Toxic.
20:
879­
882.
Elliott,
L.
F.,
Schuman,
G.
E.,
and
Viets,
F.
G.,
Jr.
1971.
Volatilization
of
nitrogen­
containing
compounds
from
beef
cattle
areas.
Soil
Sci.
Soc.
Amer.
Proc.
35:
752­
755.

Emerson,
K.,
Russo,
R.
C.,
Lund,
R.
E.,
and
Thurston,
R.
V.
1975.
Aqeuous
ammonia
equilibrium
calculations:
effect
of
pH
and
temperature.
J.
Fish.
Res.
Board
Can.
32:
2379­
2383.

Environment
Canada.
1984.
Technical
Information
for
Problem
Spills:
Ammonia.
Ottawa,
Ontario:
Environmental
Protection
Service,
Technical
Services
Branch.

Epifanio,
C.
E.
and
Srna,
R.
F.
1975.
Toxicity
of
ammonia,
nitrite
ion,
nitrate
ion,
and
orthophosphate
to
Mercenaria
mercenaria
and
Crassostrea
virginica.
Mar.
Biol.
33:
241­
246.

Erickson,
R.
J.
1985.
An
evaluation
of
mathematical
models
for
the
effects
of
pH
and
temperature
on
ammonia
toxicity
to
aquatic
organisms.
Water
Res.
19(
8):
1047­
1058.

European
Commission.
1996.
Ammonia.
International
Uniform
Chemical
Information
Database.

Evans,
D.
E.
and
Cameron,
J.
N.
1956.
Gi11
ammonia
transport.
J.
Exp.
Zool.
239:
17­
23.

Fazekas,
I.
G.
1954.
Enlargement
of
the
parathyroids
by
treatment
with
simple
acidotic
compounds.
Arch.
Pathol.
Anat.
Physiol.
324:
531­
542.
Fazekas,
I.
G.
1939.
Experimental
suprarenal
hypertrophy
induced
by
ammonium
hydroxide.
Endokrinologie.
21:
315­
337.
(
German).

Fazekas,
J.
F.
1949.
Experimental
data
on
influencing
ovarian
functions
by
simple
compounds.
Orv.
Hetil.
90:
77­
781.

Fazekas,
J.
F.,
Ticktin,
H.
E.,
Ehrmantraut,
W.
R.,
and
Alman,
R.
W.
1956.
Cerebral
metabolism
in
hepatic
insufficiency.
Amer.
J.
Med.
21:
843­
849.

Felipo,
V.,
Minana,
M.
D.,
and
Grisolia,
S.
1988.
Paradoxical
protection
of
both
protein­
free
and
high
protein
diets
against
acute
ammonium
intoxication.
Biochem.
Biophys.
Res.
Commun.
156:
506­
510.

Fenn,
L.
B.
and
Hossner,
L.
R.
1985.
Ammonia
volatilization
from
ammonium
or
ammonium­
forming
nitrogen
fertilizers.
Adv.
Soil
Sci.
1:
123­
169.

Ferguson,
W.
S.,
Koch,
W.
C.,
Webster,
L.
B.,
and
Gould,
J.
R.
1977.
Human
physiological
response
and
adaptation
to
ammonia.
J.
Occup.
Med.
19:
319­
326.

Feth,
J.
H.
1966.
Nitrogen
compounds
in
natural
water
 
A
review.
Water
Resources
Res.
2:
41­
58.

Flaks,
A.,
Hamilton,
J.
M.,
and
Clayson,
D.
B.
1973.
Effect
of
ammonium
chloride
on
incidence
of
bladder
tumors
induced
by
4­
ethylsulfonylnaphthalene­
1
sulfonamide.
J.
Natl.
Cancer
Inst.
51:
2007­
2008.

Flis,
J.
1963.
Anatomicohistopathological
changes
induced
in
carp
(
Cyrinus
carpio
L.)
by
ammonia
water.
Part
II.
Effects
of
subtoxic
concentrations.
Acta.
Hydrobiol.
10:
225­
238.

Flury,
K.
E.,
Dines,
P.
E.,
Rodarte,
J.
R.,
and
Rodgers,
R.
1983.
Airway
obstruction
due
to
inhalation
of
ammonia.
Mayo.
Clin.
Proc.
58:
389­
393.

Frank,
J.
F.
1948.
The
toxicity
of
sodium
chlorate
herbicides.
Can.
J.
Comp.
Med.
12:
216­
218.

Freedman,
L.
R.
and
Beeson,
P.
B.
1961.
Experimental
pyelonephritis
 
VIII.
The
effect
of
acidifying
agents
on
susceptibility
to
infection.
Yale
J.
Biol.
Med.
33:
318­
332.

Frizzola,
J.
B.
and
Baier,
J.
H.
1975.
Contaminants
in
rainwater
and
their
relation
to
water
quality.
Water
Sewage
Works
122:
94­
95.

Fuhrer,
J.
1985.
Formation
of
secondary
air
pollutants
and
their
occurrence
in
Europe.
Experientia
(
Basel)
41:
286­
301.

Gaafar,
H.,
Girgis,
R.,
Hussein,
M.,
and
El­
Nemr,
F.
1992.
The
effect
of
ammonia
on
the
respiratory
nasal
mucosa
of
mice.
A
histological
and
histochemical
study.
Acta.
Otolaryngol.
112:
339­
342.

Ganong,
W.
F.
1985.
Gas
transport
between
the
lung
and
tissues.
Review
of
Medical
Physiology.
12th
ed.

Gargas,
M.
L.
and
Andersen,
M.
E.
1988.
Physiologically
based
approaches
for
examining
the
pharmacokinetics
of
inhaled
vapors.
In
Gardner,
D.
E.,
Carpo,
J.
D.,
Massaro,
E.
J.,
(
eds.).
Toxicology
of
the
Lung.
New
York:
Raven
Press.
Gerald,
M.
J.
and
Slot,
M.
D.
1938.
Ammonia
gas
burns
(
an
account
of
six
cases).
Lancet
Pp.
1356­
1357.

Gersich,
F.
M.
and
Hopkins,
D.
L.
1986.
Site­
specific
acute
and
chronic
toxicity
of
ammonia
to
Daphnia
magna
Straus.
Environ.
Toxicol.
Chem.
5:
443­
447.

Gibson,
G.
E.,
Zimber,
A.,
Krook,
L.,
and
Visek,
W.
J.
1971.
Nucleic
acids
and
brain
intestinal
lesions
in
ammonia
intoxicated
mice.
Nutrition
578ABS:
2145.

Giguz,
T.
L.
1968.
Effect
of
low
concentrations
of
ammonia
and
nitrogen
oxides
in
adolescents
undergoing
vocational
training
in
the
chemical
industry.
Hyg.
Sanit.
33:
491­
434.

Gilliam,
J.
W.,
Daniels,
R.
B.
and
Lutz,
J.
F.
1974.
Nitrogen
content
of
shallow
ground
water
in
the
North
Carolina
coastal
plain.
J.
Environ.
Qual.
3:
147­
151.

Glasglow,
A.
M.
1983.
Hypoglycin
toxicity:
Studies
of
ammonia
metabolism.
Biochem.
Pharmacol.
32:
746­
748.

Goldhaber,
M.
B.
and
Kaplan,
I.
R.
1974.
The
sulfur
cycle.
In:
Goldberg,
D.
(
ed.).
The
Sea:
Ideas
and
Observations
in
Progress
in
the
Study
of
the
Seas.
Vol.
5,
New
York:
John
Wiley
Sons,
Inc.
Pp.
569­
655.

Goldman,
A.
S.
and
Yakovac,
W.
C.
1964.
Salicylate
intoxication
and
congenital
anomalies.
Arch.
Env.
Health
8:
648­
656.

Gomaa,
H.
M.
and
LeBlanc,
J.
R.
1985.
Environmental
considerations
for
the
nitrogen
fertilizer
industry.
Fertilizer
News
30:
23­
37.

Gould,
W.
D.,
Hagedorn,
C.,
and
McCready,
R.
G.
L.
1986.
Urea
transformations
and
fertilizer
efficiency
in
soil.
Adv.
Agronomy
40:
209­
238.

Graedel,
T.
E.
and
Allara,
D.
L.
1977.
Pp.
467­
473.
EPA­
600/
3­
77­
001A,
PB­
264.

Grayson,
M.
1978.
Kirk­
Othmer
Encyclopedia
of
Chemical
Technology.
Vol.
2,
New
York:
John
Wiley
and
Sons,
Inc.

Greenstein,
J.
P.,
Winitz,
M.,
Gullino,
P.,
Bimbaum,
S.
M.,
and
Otey,
M.
C.
1956.
Studies
on
the
metabolism
of
amino
acids
&
related
compound
in
Vivo­
Prevention
of
ammonia
toxicity
by
arginine
&
related
compounds.
Arch.
Biochem.
Biophys.
64:
342­
354.

Griffiths,
R.
F.
and
Megson,
L.
C.
1984.
The
effect
of
uncertain
ties
human
toxic
response
on
hazard
range
estimation
for
ammonia
and
chlorine.
Atmos.
Environ.
18:
1195­
1206.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Grossi,
C.
E.,
Prytz,
B.,
and
Rousselot,
L.
M.
1968.
Adensoine
triphosphate
in
prevention
of
ammonia
intoxication
in
dogs.
Surgical
Forum
19:
363­
369.

Gupta,
B.
N.,
Khanna,
R.
N.,
and
Datta,
K.
K.
1979.
Toxicological
studies
of
ammonium
sulfamate
in
rat
after
repeated
oral
administration.
Toxicol.
13:
45­
49.
Hales,
J.
M.
and
Drewes,
D.
R.
1979.
Solubility
of
ammonia
in
water
at
low
concentrations.
Atmos.
Environ.
13:
1133­
1147.

Hanst,
P.
L.,
Wong,
N.
W.,
and
Bragin,
J.
1982.
A
long­
path
infra­
red
study
of
Los
Angeles
smog.
Atmos.
Environ.
15:
969­
981.

Harader,
R.
R.
and
Allen,
G.
H.
1983.
Ammonia
toxicity
to
chinook
salmon
parr:
reduction
in
saline
water.
Trans.
Amer.
Fish.
Soc.
112:
834­
837.

Harris,
D.
J.,
Siegel,
E.,
and
Baska,
R.
D.
1987.
Transient
hyperammonaemia.
A.
Inher.
Metab.
Dis.
10:
269.

Harward,
C.
N.,
McClenny,
W.
A.,
Hoell,
J.
M.,
Williams,
J.
A.,
and
Williams,
B.
S.
1982.
Ambient
ammonia
measurements
in
coastal
southeastern
Virginia.
Atmos.
Env.
16:
2496­
2500.

Haussinger,
D.
1986.
Regulation
of
hepatic
ammonia
metabolism:
the
intercellular
glutamine
cycle.
Adv.
Enzyme
Regue.
25:
159­
180.

Haussinger,
D.
and
Gerok.
1986.
Metabolism
of
amino
acids
and
ammonia.
In
Thurma,
R.
G.,
Kauffman,
F.
C.,
Jungermann,
K.
(
eds.).
Regulation
of
Hepatic
Metabolism:
Intra
and
Intercellular
Compartmentation.
New
York:
Plenum
Publ.
Pp.
253­
291.
Hawkins,
R.
A.,
Miller,
A.
L.,
Nielsen,
R.
C.,
and
Veech,
R.
L.
1973.
The
acute
action
of
ammonia
on
rat
brain
metabolism
in
vivo.
Biochem.
J.
134:
1001­
1008.

Hazardous
Substances
Data
Bank
(
HSDB).
1999.
Ammonia.
National
Library
of
Medicine,
Bethesda,
MD.

Helmers,
S.,
Top,
F.
H.,
and
Knapp,
L.
W.
1971.
Ammonia
injuries
in
agriculture.
J.
Iowa
Med.
Soc.
61:
271­
280.

Hemens,
J.
1965.
The
Institute
of
Sewage
Purification
Southern
African
branch
memorandum
to
the
honourable
the
minister
of
water
affairs,
on
effluent
standards.
J.
Inst.
Sew.
Purif.
65:
265­
271.

Herbert,
D.
W.
M.
and
Shurben,
D.
S.
1964.
The
toxicity
to
fish
of
mixtures
of
poisons.
I.
Salts
of
ammonia
and
zinc.
Ann.
App.
Biol.
53:
33­
41.

Hermann,
G.
and
Viehrig,
J.
1979.
[
Irritative
respiratory
tract
diseases
from
ammonia
and
sulfuric
acid
aerosols
in
a
cuprammonium
rayon
spinning
mill].
Z.
Gesamte
Hyg.
25:
581­
584.
(
Russian).

Hermanutz,
R.
O.,
Hedtke,
S.
F.,
Arthur,
J.
W.,
Andrew,
R.
W.,
Allen,
K.
N.,
and
Helgen,
J.
C.
1987.
Ammonia
effects
on
microinvertebrates
and
fish
in
outdoor
experimental
streams.
Environ.
Poll.
47:
249­
283.

Heuner,
A.,
Azar,
C.,
Mildenberger,
S.,
and
Silbernagl,
S.
1988.
Renal
handling
of
glutathione.
Contr.
Nephrol.
63:
49­
52.

Heymans,
C.
and
Es,
J.
J.
1949.
Blood
Changes
due
to
ammonia
inhalation?
Science
109:
121­
122.
Hickey,
C.
W.
and
Vickers,
M.
L.
1994.
Toxicity
of
ammonia
to
nine
native
New
Zealand
freshwater
invertebrate
species.
Arch.
Environ.
Contam.
Toxicol.
26:
292­
298.

Hidy,
G.
M.
1974.
Characterization
of
Aerosols
in
California
(
ACHEX).
Thousand
Oaks,
California:
Rockwell
International.

Hillaby,
B.
A.
and
Randall,
D.
J.
1979.
Acute
ammonia
toxicity
and
ammonia
excretion
in
rainbow
trout
(
Salmo
gairdneri).
J.
Fish.
Res.
Board
Can.
36:
621­
629.

Hoeft,
R.
G.,
Kenney,
D.
R.,
and
Walsh,
L.
M.
1972.
Nitrogen
and
sulfur
in
precipitation
and
sulfur
dioxide
in
the
atmosphere
in
Wisconsin.
J.
Environ.
Qual.
1:
203­
208.

Holness,
D.
L.,
Prudham,
J.
T.,
and
Nethercott,
J.
R.
1989.
Acute
and
chronic
respiratory
effects
of
occupational
exposure
to
ammonia.
Am.
Iond.
Hyg.
Assoc.
J.
50(
12):
646­
650.

Hou,
S.,
Zhang,
Y.
G.,
Lao,
D.
F.,
He.,
J.,
and
Zhong,
N.
S.
1991.
The
chronic
latent
lung
function
damage
in
workers
exposed
to
mild
ammonia
pollution.
Am.
Rev.
Resp.
Dis.
143(
4pt.
2):
A105.

Hunt,
R.
D.
1977.
Measurement
of
partial
pressure
of
ammonia
in
breath
and
arterial
blood.
Evidence
for
secretion
of
ammonia
into
expired
gas
by
metabolism
of
monoamines
in
the
lung.
Diss.
Abstr.
Int.
Ser.
B.
37:
3279.

Hutchinson,
G.
L.,
Mosier,
A.
R.,
and
Andre,
C.
E.
1982.
Ammonia
and
amine
emissions
from
a
large
cattle
feedlot.
J.
Environ.
Qual.
11:
288­
293.

Hutchinson,
G.
L.
and
Viets,
F.
G.,
Jr.
1969.
Nitrogen
enrichment
of
surface
water
by
absorption
of
ammonia
volatilized
from
cattle
feedlots.
Science
166:
514­
515.

Iles,
J.
F.
and
Jack,
J.
J.
B.
1980.
Ammonia:
Assessment
of
its
action
on
postsynaptic
inhibition
as
a
cause
of
convulsion.
Brain
103:
555­
578.

Indorato,
A.
M.
et
al.
1985.
First
International
Symposium
on
Toxicity
Testing
using
Bacteria.
Burlington,
Ontario,
Canada
17­
19
May
1983.
In
Datenblaetter
zum
Katalog
wassergefaehrdender
Stoffe,
KBwS.
Nr.
5.
In
European
Commission.
1996.
Ammonia.
International
Uniform
Chemical
Information
Database.

Industrial
BIO­
TEST
Laboratories,
Inc.
1973.
Report
to
International
Institute
of
Ammonia
Refrigeration
 
Irritation
Threshold
Evaluation
Study
with
Ammonia.
March
23.
IBT
No.
663­
03161.

Institution
of
Chemical
Engineers.
1988.
Second
Report
on
the
Toxicity
Working
Party.
Ammonia
Toxicity
Monograph.
Warwidkshire,
England.

Jacobsohn,
D.
and
Jorgensen,
C.
B.
1955.
Survival
and
function
of
auto­
and
homografts
of
adenohypophysial
tissue
in
the
toad,
Bufo
bufo
(
L).
Acta.
Phys.
Scandinav.
36:
1­
12.

Jaffe,
H.
L.,
Bodansky,
A.,
and
Blair,
J.
E.
1932.
The
effects
of
parathormone
and
ammonium
chloride
on
the
bones
of
rabbits.
J.
Exp.
Med.
55:
695­
701.
Jaffe,
H.
L.,
Bodansky,
A.,
and
Chandler,
J.
P.
1932.
Ammonium
chloride
decalcification,
as
modified
by
calcium
intake:
The
relation
between
generalized
osteoporosis
and
ostitis
fibrosa.
J.
Exp.
Med.
56:
823­
834.

Janicki,
R.
H.
1970.
Renal
adaptation
during
chronic
NH4Cl
acidosis
in
the
rat:
No
role
for
hyperplasia.
Am.
J.
Physiol.
219:
613­
618.

Jofre,
M.
B.,
and
Karasov,
W.
H.
1999.
Direct
effect
of
ammonia
on
three
species
of
North
American
anuran
amphibians.
Environ.
Toxicol.
Chem.
18(
8):
1806­
1812.

Jojima,
T.
and
Sato,
T.
1975.
Pollution
abatement
in
a
urea
plant.
Chem.
Age
India.
26:
524­
529.

Jones,
K.
1973.
Ammonia.
In
Bailar,
Jr.,
J.
C.,
Emeleus,
H.
J.,
Nyhom,
R.,
Trotman­
Dickenson,
A.
F.
(
eds.).
Comprehensive
Inorganic
Chemistry.
Vol.
2,
New
York:
Pergammon
Press.
Pp.
199­
227.

Jones,
S.
R.
1945.
Ammonia
and
the
nervous
system.
J.
Am.
Med.
Assoc.
Pp.
620.

Jungermann,
K.
1988.
Metabolic
zonation
of
liver
parenchyma.
Semin.
Liver
Dis.
8:
329­
341.
Kagota,
K.,
Iwase,
T.,
and
Kojima,
T.
1979.
Diammonium
citrate
addition
to
a
diet
restricted
nonessential
amino
acids
for
young
pigs.
Jap.
J.
Vet.
Sci.
41:
131­
138.

Kane,
L.
E.,
Barrow,
C.
S.,
and
Alarie,
Y.
1979.
A
short­
term
test
to
predict
acceptable
levels
of
exposure
to
airborne
sensory
irritants.
Amer.
Inc.
Hyg.
Assoc.
40:
207­
229.

Kane,
L.
E.
Dombroske,
R.,
and
Alarie,
Y.
1980.
Evaluation
of
sensory
irritation
from
some
common
industrial
solvents.
Am.
Ind.
Hyg.
Assoc.
J.
41:
451­
455.

Kaniewska­
Purs,
M.
1982.
The
effect
of
ammonia,
chlorine,
and
chloramine
toxicity
on
the
mortality
of
Daphnia
magna
straus.
Pol.
Arch.
Hydrobiol.
29(
3­
4):
607­
624.

Kapeghian,
J.
C.,
Jones,
A.
B.,
and
Water,
I.
W.
1985.
Effects
of
ammonia
on
selected
hepatic
microsomal
enzyme
activity
in
mice.
Bull.
Environ.
Contam.
Toxicol.
35:
15­
22.

Kapeghian,
J.
C.,
Mincer,
H.
H.,
Jones,
A.
B.,
Verlangieri,
A.
J.,
and
Waters,
I.
W.
1982.
Acute
inhalation
toxicity
of
ammonia
in
mice.
Bull.
Environ.
Contam.
Toxicol.
29:
371­
378.

Karasawa,
Y.
1986.
Ammonia
production
and
its
contribution
to
urinary
nitrogenous
compounds
in
chickens
fed
low
or
high
protein
diet.
J.
Nutr.
116:
2378­
2386.

Karasawa,
Y.,
1984.
Ammonia
absorption
from
the
intestine
in
adult
cockerels.
Comp.
Biochem.
Physiol.
A.
77:
139­
141.

Kass,
I.,
Zamel,
N.,
Droby,
C.
A.
et
al.
1972.
Bronchlectasis
following
ammonia
burns
of
the
respiratory
tract.
Chest
62:
282­
285.

Katz,
B.
1979.
Relationship
of
the
physiology
of
aquatic
organisms
to
the
lethality
of
toxicants:
a
broad
overview
with
emphasis
on
membrane
permeability.
Aquatic
Toxicology.
ASTM
STP
667.
Marking,
L.
L.
and
Kimerle,
R.
A.
(
eds.).
American
Society
for
Testing
and
Materials.
Pp.
62­
76.
Kazasov,
P.
L.
et
al.
1971.
Study
of
air
pollution
around
the
nitrogen
fertilizer
plant
near
Stara
Zayora
and
its
effects
on
the
health
of
the
population.
Khig
Zdraveopa.
14:
45­
52.

Keeney,
D.
R.
1972.
The
Fate
of
Nitrogen
in
Aquatic
Ecosystems.
Literature
Review
No.
3.
Madison:
University
of
Wisconsin
Water
Resources
Center.

Keeney,
D.
R.
and
Sahrawat,
K.
L.
1986.
Nitrogen
transformations
in
flooded
rice
soils.
Fertilizer
Res.
9:
15­
38.

Kemp,
M.
B.
and
Quayle,
J.
R.
1966.
Microbial
growth
on
C1
compounds.
Biochem.
J.
99:
41­
48.

Keplinger,
M.
L.
1973.
Report
to
International
Institute
of
Ammonia
Refrigeration:
Irritation
Threshold
Evaluation
Study
With
Ammonia.
Industrial
BIOTEST
Laboratories,
Inc.

Keplinger,
M.
L.,
Schadeberg,
K.
J.,
Goode,
J.
W.,
and
Calandra,
J.
C.
1973.
Irritation
threshold
evaluation
study
with
ammonia.
Report
to
the
Institute
of
Ammonia
Refrigeration.
Northbrook,
Illinois.

Keyes,
W.
F.
1975.
Nitrogen.
In
Mineral
facts
and
problems.
Washington
DC:
US
Department
of
the
Interior.
P.
749­
760.
In
World
Health
Organization
(
WHO).
1986.
Ammonia­
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Kfir,
R.
and
Prozesky,
O.
W.
1981.
Detection
of
toxic
substances
in
water
by
means
of
a
mammalian
cell
culture
technique.
Water
Res.
15:
553­
559.

King,
P.
A.
and
Goldstein,
L.
1985.
Renal
excretion
of
nitrogenous
compounds
invertebrates.
Renal
Physiol.
8:
261­
278.

Kirschner,
E.
M.
1996.
Growth
of
top
50
chemicals
slowed
in
1995
from
very
high
1994
rate.
Chem.
Eng.
News.
74:
16­
22.

Klassen,
C.
D.,
Amdur,
M.
O.,
and
Doull,
J.
1986.
Casarett
and
Doull's
Toxicology.
3rd
ed.
New
York:
Pergamon
Press.

Klontz,
G.
W.,
Stewart,
B.
C.,
and
Eib,
D.
W.
1985.
On
the
etiology
and
pathophysiology
of
environmental
gill
disease
in
juvenile
salmonids.
Fish
Shellfish
Pathol.
199­
210.

Kluesenser,
J.
W.
and
Lee,
G.
F.
1974.
Nutrient
loading
from
a
separate
storm
sewer
in
Madison,
Wisconsin.
J.
Water
Pollut.
Control
Fed.
46:
920­
936.

Knepp,
G.
L.
and
Arkin,
G.
F.
1973.
Ammonia
toxicity
levels
and
nitrate
tolerance
of
channel
catfish.
Prog.
Fish­
Cult.
35(
4):
221­
224.

Knepper,
M.
A.,
Packer,
R.,
and
Good,
D.
W.
1989.
Ammonium
transport
in
the
kidney.
Physiol.
Rev.
69:
179­
249.

Knoph,
M.
B.
1992.
Acute
toxicity
of
ammonia
to
Atlantic
Salmon
(
Salmo
salar)
parr.
Comp.
Biochem.
Physiol.
101C(
2):
275­
282.
Koenig,
H.
and
Koenig,
R.
1949.
Production
of
acute
pulmonary
edema
by
ammonium
salts.
Proc.
Soc.
Exp.
Biol.
Med.
70:
375­
380.

Kowalenko,
C.
G.
1978.
Organic
Nitrogen,
Phosphorus
and
Sulfur
in
Soils.
In:
Schnitzer,
M.
and
Khan,
S.
U.
(
eds.).
Soil
Organic
Matter.
New
York:
Scientific
Publishing
Co.

Kulasek,
G.,
Siwecka,
B.,
Owczarczyk,
B.,
Rynca,
J.,
Barej,
W.
1975.
Effect
of
protein
level
and
NH4Cl
addition
to
diet
on
ammonia
detoxication
in
rats.
Zb.
Vet.
Med.
A.
22:
215­
223.

L'Abbe,
K.
A.
and
Hoey,
J.
R.
1984.
Review
of
the
health
effects
of
urea­
formaldehyde
foam
insulation.
Environ.
Res.
35:
246­
263.

La
Rosa,
M.,
Mancuso,
G.
R.,
Greco,
D.,
Di
Paola,
M.,
Schiliro,
G.,
Bagnato,
G.
F.,
and
Bellanti,
J.
A.
1991.
Studies
of
delayed
hypersensitivity
responses
in
children
in
an
industrialized
region
of
region.
Annals
Allergy.
66:
481­
484.

Lagerstrand,
L.
and
Hedenstierna,
G.
1990.
Gas­
exchange
impairment:
its
correlation
to
lung
mechanics
in
acute
airway
obstruction
(
studies
on
a
rabbit
asthma
model).
Clin.
Physiol.
10:
363­
380.

Lagler,
K.
F.,
Bardach,
J.
E.,
Miller,
R.
R.,
and
Passino,
D.
R.
M.
1977.
Ichthyology.
2nd
ed.
New
York:
John
Wiley
and
Sons,
Inc.
Pp.
506.

Landahl,
H.
D.
and
Herrmann,
R.
G.
1950.
Retention
of
vapors
and
gases
in
the
human
nose
and
lung.
Ind.
Hyg.
Occup.
Med.
Pp.
36­
45.

Landfair,
S.
W.,
Brown,
E.
L.,
Israel,
R.
J.,
and
Kreis,
P.
B.
1993.
The
EC's
seventh
amendment:
Fundamental
changes
in
the
regulation
of
chemical
substances
manufactured
and
imported
into
the
European
community.
International
Environmental
Reporter
0149­
8738/
93:
785­
790.

Langowska,
I.,
and
Moskal,
J.
1974.
Effect
of
ammonia
and
urea
on
nitrobacteria
in
water
environment.
Pol.
Arch.
Hydrobiol.
21(
1):
119­
123.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Langton,
J.
A.,
Murphy,
P.
J.,
Barker,
P.,
Key,
A.,
and
Smith,
G.
1993.
Measurement
of
the
sensitivity
of
upper
airway
reflexed.
Br.
J.
Anaesthesia
70:
126­
130.

Larson,
T.
V.,
Covert,
D.
S.,
Frank,
R.,
and
Charlson,
R.
J.
1977.
Ammonia
in
the
human
airways:
Neutralization
of
inspired
acid
sulfate
aerosois.
Science
197:
161­
163.

LeDuc,
D.,
Gris,
P.,
Lheureux,
P.,
Gevenois,
P.
A.,
DeVuyst,
P.,
and
Yernault,
J.
C.
1992.
Acute
and
long
term
respiratory
damage
following
inhalation
of
ammonia.
Thorax
47:
755­
757.

Legters,
L.
1980.
Biological
Effects
of
Short,
High­
Level
Exposure
to
Gases:
Ammonia.
Prepared
by
Enviro
Control,
Inc.
for
the
U.
S.
Army
Medical
Research
and
Development
Command.
May.
Contract
number:
DAMD17­
79­
C­
9086.
Lehmann,
K.
B.
1986.
Experimental
studies
on
the
effects
of
technically
and
hygienically
important
gases
and
vapors
on
the
organism
(
Parts
I
&
II
Ammonia
and
Hydrogen
Chloride).
Arch.
Hyg.
5:
1­
126.

Leonardos,
G.
D.,
Kendal,
D.,
and
Barnard,
N.
1969.
Odor
threshold
determinations
of
53
odorant
chemicals.
J.
Air
Poll.
Control
Assoc.
19:
91­
95.

Leuven,
R.
S.
E.
W.
and
Shuurkes,
J.
A.
A.
R.
1984.
Effects
of
acid
precipitation
containing
sulfur
and
nitrogen
on
weakly
buffered
waters
low
in
nutrients.
The
Netherlands,
Laboratory
of
Aquatic
Ecology,
University
of
Nymegen,
and
Ministry
of
Housing,
Physical
Planning
and
Environment.

Levy,
D.
M.,
Divertie,
M.
B.,
Litzow,
T.
J.
and
Henderson,
J.
W.
1964.
Ammonia
burns
of
the
face
and
respiratory
tract.
J.
Am.
Med.
Assoc.
190:
95­
98.

Lide,
D.
R.
(
ed.).
1999.
CRC
Handbook
of
Chemistry
and
Physics.
80th
Edition.
Boston:
CRC
Press.

Liebhardt,
W.
C.,
Golt,
C.,
and
Tupin,
J.
1979.
Nitrate
and
ammonium
concentrations
of
ground
water
resulting
from
poultry
manure
applications.
J.
Environ.
Qual.
8:
211­
215.

Lin,
S.
and
Raabe,
W.
1985.
Ammonia
Intoxication:
Effects
on
cerebral
cortex
and
spinal
cord.
J.
Neurochem.
44:
1252­
1258.
Linden,
E.,
Bengtsson,
B.
E.,
Svanberg,
O.,
and
Sundstrom,
G.
1979.
The
acute
toxicity
of
78
chemicals
and
pesticide
formulations
against
two
brackish
water
organisms,
the
bleak
(
Alburnus
alburnus)
and
the
harpacticoid
(
Nitocra
spinipes).
Chemosphere
11/
12:
843­
851.

Litton
Bionetics,
Inc.
prepared
for
the
FDA.
1975.
Mutagenic
Evaluation
of
Compound
FDA73­
42,
Ammonium
Sulfate
Granular,
Food
Grade,
Washington
D.
C.
Litton
Bionetics.
(
NTIS
PB­
245506).
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Llinas,
R.,
Baker,
R.,
and
Precht,
W.
1974.
Blockage
of
inhibition
by
ammonium
acetate
action
on
chloride
pump
in
cat
trochlear
motorneurons.
J.
Neurophysiol.
37:
522­
533.

Lockwood,
A.
H.,
McDonald,
J.
M.,
Reiman,
R.
E.,
Gelbard,
A.
S.,
Laughlin,
J.
S.,
Duffy,
T.
E.,
and
Plum,
F.
1979.
The
dynamics
of
ammonia
metabolism
in
man.
Effects
of
liver
disease
and
hyperammonia.
J.
Clin.
Invest.
Pp.
63.

Loehr,
R.
C.
1974.
Characteristics
and
comparative
magnitude
of
non­
point
sources.
J.
Water
Pollut.
Control
Fed.
46(
8):
1849­
1872.

Lotspeich,
W.
D.
1965.
Renal
hypertrophy
in
metabolic
acidosis
and
its
relation
to
ammonia
excretion.
Am.
J.
Physiol.
208:
1135­
1142.
Lundholm,
M.,
Laverll,
G.,
and
Mathiasson,
L.
1990.
Self­
leveling
mortar
as
a
possible
cause
of
symptoms
associated
with
"
sick
building
syndrome."
Arch.
Environm.
Health
45(
3):
135­
140.

Lux,
H.
D.
1971.
Ammonium
and
chloride
extrusion:
Hyperpolarizing
synaptic
inhibition
in
spinal
motoneurons.
Science
Pp.
555­
557.

Lux,
H.
D.,
Loracher,
C.,
and
Neher,
E.
1970.
The
action
of
ammonium
on
postsynaptic
inhibition
of
cat
spinal
motorneurons.
Exp.
Brain
Res.
11:
431­
447.

Maetz,
J.
1973.
Na+/
NH4
+,
Na+/
H+
exchanges
and
NH3
movement
across
the
gill
of
Carassius
auratus.
J.
Exp.
Biol.
58:
255­
275.

MacEwen,
J.
D.,
Theodore,
J.,
and
Vernot,
E.
H.
1970.
Human
exposure
to
EEL
concentrations
of
monomethlyhydrazine.
Proceedings
of
the
1st
Annual
Conference
on
Environmental
Toxicology,
Ohio,
Wright­
Patterson
Air
Force
Base,
9­
11
September,
1970.
Pp.
355­
363.

MacEwen,
J.
D.
and
Vernot,
E.
H.
1972.
Toxic
hazards
research
unit
annual
technical
report.
NTIS
AD
755­
358.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Mackay,
D.,
Shiu,
W.
Y.,
and
Ma,
K.
C.
1994.
Level
I,
II
and
III
Fugacity
Calculations
for
the
Illustrated
Handbook
for
Physical­
chemical
Properties
and
Environmental
Fate
for
Organic
Chemicals.
New
York:
CRC
Press.

Maguire,
M.
P.
1974.
Chemically
induced
abnormal
chromosome
behavior
at
meiosis
in
maize.
Chromosoma
42:
213­
223.
Manninen,
A.,
Anttiia,
S.,
and
Savoiainen,
H.
1988.
Rat
metabolic
adaptation
to
ammonia
inhalation
(
42664).
Proc.
Soc.
Exp.
Biol.
Med.
187:
278­
281.

Marsalek,
J.,
and
Ng,
H.
Y.
F.
1989.
Evaluation
of
pollution
loadings
from
urban
nonpoint
sources:
Methodology
and
applications.
J.
Great
Lakes
Res.
15(
3):
444­
451.
In
Constable,
M.,
Jensen,
F.,
McLernon,
J.
Craig,
G.,
Moore,
D.
1999.
Canadian
Environmental
Protection
Act,
Priority
Substances
List
II:
Supporting
Document
for
Ammonia
in
the
Aquatic
Environment.
Draft,
Unpublished
Version.
Government
of
Canada,
Environment
Canada.
Government
of
Canada,
Environment
Canada.

Marschang,
F.
and
Crainiceanu,
E.
1971.
Investigations
on
the
connections
between
cowshed
climate
and
calf
mortality.
Berlin
Muchen
Tieraerzti
Wochenschr.
84:
417­
450.
(
German).

Marschang,
F.
and
Petre,
C.
1971.
The
NH3
content
of
cowshed
air
and
its
influence
on
morbidity
and
animal
losses
in
cattle­
fattening
pens.
Zentralbl
Veternaermed.
18:
646­
654.
(
Original
in
German).

Martin,
J.
L.
1979.
Review
of
literature
on
the
toxicity
of
ammonia.
For
the
Fertilizer
Institute.

Matasa,
C.
and
Matasa,
E.
1968.
L'industrie
moderne
des
produitsazotes.
Paris,
France.
Pp.
63­
208.
Matheson,
D.
F.
and
Van
Den
Berg,
C.
J.
1975.
Ammonia
and
Brain
glutamine:
inhibition
of
glutamine
degradation
by
ammonia.
Biochem.
Soc.
Trans
 
559th
Meeting.
3:
525­
528.

Matsumoto,
S.
1989.
Effects
of
ammonia
and
histamine
on
lung
irritant
receptors
in
the
rabbit.
Respir.
Physio.
77:
301­
308.

Mayan,
M.
H.
and
Merilan,
C.
P.
1972.
Effects
of
ammonia
inhalation
on
respiration
rate
of
rabbits.
J.
Anim.
Sci.
34:
448­
452.

Mayes,
M.
A.,
Alexander,
H.
C.,
Hopkins,
D.
L.,
and
Latvaitas,
P.
B.
1986.
Acute
and
chronic
toxicity
of
ammonia
to
freshwater
fish:
a
site­
specific
study.
Environ.
Toxicol.
Chem.
5:
437­
442.

McCandles,
D.
W.
and
Schenkers,
S.
1981.
Effect
of
acute
ammonia
toxication
on
energy
stores
in
the
cerebral
reticular
activating
system.
Exp.
Brain
Res.
4:
325­
330.

McCormick,
J.
H.,
Broderius,
S.
J.,
and
Fiandt,
J.
T.
1984.
Toxicity
of
ammonia
to
early
life
stages
of
the
green
sunfish
Lepomis
cyanellus.
Environ.
Poll.
(
Series
A)
36:
147­
163.

McKay,
H.
A.
C.
1969.
Ammonia
and
air
pollution.
Chem.
Ind.
34:
1162­
1165.

McKhann,
G.
M.
and
Tower,
D.
B.
1961.
Ammonia
toxicity
and
cerebral
oxidative
metabolism.
Am.
J.
Physiol.
200:
420­
424.

McLean,
J.
A.,
Mathews,
K.
P.,
Brayton,
P.
R.,
et
al.
1979.
Effect
of
ammonia
on
nasal
resistance
in
atopic
and
nonatopic
subjects.
Ann.
Otol.
Rhinol.
Laryngol.
88:
228­
234.

Mead,
J.
W.
1985.
Allowable
ammonia
for
fish
culture.
Prog.
Fish­
Cult.
47(
3):
135­
145.

Mikhailuts,
A.
P.,
Poponnikox,
V.
A.,
Goluber,
L.
V.,
and
Lambina,
S.
A.
1979.
Effects
of
ammonia
and
nitrogen
dioxide
on
respiratory
microbial
infections.
Gig.
Sanit.
8:
8­
11.
(
Russian).

Miles,
F.
D.
1961.
Nitric
Acid
Manufacture
and
Uses.
London:
Oxford
University
Press.

Miller,
D.
C.,
Poucher,
S.,
Cardin,
J.
A.,
and
Hansen,
D.
1990.
The
acute
and
chronic
toxicity
of
ammonia
to
marine
fish
and
a
mysid.
Arch.
Environ.
Contam.
Toxicol.
19:
40­
48.

Miller,
T.
J.
1973.
Cleft
palate
formation:
the
effects
of
fasting
and
iodacetic
acid
on
mice.
Teratology
7:
177­
181.

Minana,
M­
D.,
Felipo,
V.,
and
Grisolla,
S.
1988.
Protective
effect
of
long
term
ammonium
ingestion
against
acute
ammonium
intoxication.
Biochem
Biophys
Res.
Commun.
153:
979­
983.
Monda,
D.
P.,
Galat,
D.
L.,
Finger,
S.
E.,
and
Kaiser,
M.
S.
1995.
Acute
toxicity
of
ammonia
(
NH3­
N)
in
sewage
effluent
to
Chironomus
riparius:
II.
Using
a
generalized
linear
model.
Arch.
Environ.
Contam.
Toxicol.
28:
385­
390.

Montague,
T.
J.
and
Macnell,
A.
R.
1980.
Mass
ammonia
inhalation.
Chest
77:
496­
498.

Moore,
E.
W.,
Strohmeyer,
G.
W.,
Chalmers,
T.
C.,
Rasmusen,
I.
K.
and
Gordon,
S.
J.
1963.
Distribution
of
ammonia
across
the
blood­
cerebrospinal
fluid
barrier
in
patients
with
hepatic
failure.
J.
Am.
Med.
Assoc.
35:
350­
362.

Morris,
J.
C.
1978.
Chemistry
of
aqueous
chlorine
in
relation
to
water
chlorination.
In
Jolley,
R.
L.
(
ed.).
Water
Chlorination:
Environmental
Impact
and
Health
Effects.

Morris,
J.
G.
and
Rogers,
Q.
R.
1978.
Ammonia
intoxication
in
the
near­
adult
cat
as
a
result
of
a
dietary
deficiency
of
arginine.
Science
199:
431­
432.

Mosier,
A.
R.
1978.
Inhibition
of
photosynthesis
and
nitrogen
fixation
in
algae
by
volatile
nitrogen
bases.
J.
Environ.
Qual.
7(
2):
237­
240.

Mosier,
A.
R.,
Andres,
C.
E.,
and
Viets,
Jr.,
F.
G.
1973.
Identification
of
aliphatic
amines
volatilized
from
cattle
feedyard.
Environ.
Sci.
Tech.
7:
642­
644.

Motyl,
T.
and
Debski,
B.
1977.
The
influence
of
increasing
amounts
of
NH4CL
in
the
diet
on
ammonia
detoxication
in
rats.
Zbl
Vet.
Med.
24:
333­
342.

Mount,
D.
I.
and
Norberg,
T.
J.
1984.
A
seven­
day
life­
cycle
cladoceran
toxicity
test.
Environ.
Toxicol.
Chem.
3:
425­
434.

Mount,
D.
R.,
Gulley,
D.
D.,
Hockett,
J.
R.,
Garrison,
T.
D.,
and
Evans,
J.
M.
1997.
Statistical
models
to
predict
the
toxicity
of
major
ions
to
Ceriodaphnia
dubia,
Daphnia
magna
and
Pimephales
promelas
(
fathead
minnows).
Environ.
Toxicol.
Chem.
16(
10):
2009­
2019.

Mudge,
G.
H.
1985.
Agents
affecting
volume
and
composition
of
body
fluids.
In
Goodman
and
Gilman
(
ed.).
The
Pharmacological
Basic
of
Therapeutics.
7
ed.
New
York:
MacMillan
Publishing
Company.
Pp.
846­
879.
Mukherjee,
S.
and
Bhattacharya,
S.
1977.
Variations
in
the
hepatopancreatic
alpha­
amylase
activity
in
fishes
exposed
to
some
industrial
pollutants.
Water
Res.
11:
71­
74.

Mulhausen,
J.
R.,
McJilton,
C.
E.,
Redig,
P.
T.,
and
Janni,
K.
A.
1987.
Aspergillus
and
other
human
respiratory
disease
agents
in
Turkey
confinement
houses.
Am.
Ind.
Hyg.
Assoc.
J.
48:
894­
899.

Natarajan,
K.
V.
1970.
Toxicity
of
ammonia
to
marine
diatoms.
J.
Water
Pollut.
Control
Fed.
42(
5):
R184­
R190.

National
Institute
for
Occupational
Safety
and
Health
(
NIOSH).
1974.
Criteria
for
a
recommended
standard 
Occupational
Exposure
to
Ammonia.
National
Institute
for
Occupational
Safety
and
Health
(
NIOSH).
1997.
NIOSH
Pocket
Guide
to
Chemical
Hazards
P.
359.

National
Research
Council
(
NRC).
1977.
Ammonia.
Prepared
for
the
U.
S.
EPA.,
Office
of
Research
and
Development,
Research
Triangle
Park,
North
Carolina.
EPA­
600/
1­
77­
054.

National
Research
Council
(
NRC).
1979.
Ammonia.
Subcommittee
on
Ammonia.
Committee
on
Medical
and
Biologic
Effects
of
Environmental
Pollutants.
Division
of
Medical
Sciences,
Assembly
of
Life
Sciences.
National
Research
Council.
Baltimore:
University
Park
Press.
NTIS
No.
PB
278­
027.

Navazio,
F.,
Gerritsen,
T.,
and
Wright,
G.
J.
1961.
Relationship
of
ammonia
intoxication
to
convulsion
and
coma
in
rats.
J.
Neurochem.
8:
146­
151.

Neufeld,
R.
D.,
Hill,
A.
J.,
and
Adekoya,
D.
O.
1980.
Phenol
and
free
ammonia
inhibition
to
Nitrosomonas
activity.
Water
Res.
14(
12):
1695­
1703.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Nicholas,
D.
J.
D.
1963.
The
metabolism
of
inorganic
nitrogen
and
its
compounds
in
microorganisms
Biol.
Rev.
38:
530­
568.

Nicoli,
R.
A.
1978.
The
blockade
of
gaba
mediated
responses
in
the
frog
spinal
cord
by
ammonium
ions
and
furosemide.
J.
Physiol.
283­
121­
132.

Niden,
A.
H.
1968.
Effects
of
Ammonia
inhalation
on
the
terminal
airways.
11th
Aspen
Emphysema
Conference.
41­
44.

Nimmo,
D.
W.
R.,
Link,
D.,
Parrish,
L.
P.,
Rodriguez,
G.
J.,
and
Wuerthele,
W.
1989.
Comparison
of
on­
site
and
laboratory
toxicity
tests:
derivation
of
site­
specific
criteria
for
un­
ionized
ammonia
in
a
Colorado
transitional
stream.
Environ.
Toxicol.
Chem.
8:
1177­
1189.

Nishimura,
H.
1985.
Endocrine
control
of
renal
handling
of
solutes
and
water
in
vertebrates.
Renal
Physiol.
8:
279­
300.

Noda,
K.
and
Chikamori,
K.
1976.
Effects
of
ammonia
via
prepyriform
cortex
on
regulation
of
food
intake
in
the
rat.
Am.
J.
Physiol.
231:
1263­
1266.

Odum,
E.
P.
1971.
Fundamentals
of
Ecology.
3rd
ed.
Philadelphia,
PA:
W.
B.
Saunders
Co.
Pp.
88.

Overrein,
L.
N.,
Seip,
H.
M.,
and
Tollan,
A.
(
eds.).
1980.
Acid
Precipitation:
Effects
on
Forest
and
Fish.
Final
Report.
Oslo.
SNSF
Project
1972­
1980.

Oyetunde,
O.
O.
F.,
Thomson,
R.
G.,
and
Carlson,
H.
C.
1978.
Aerosol
exposure
of
ammonia
dust
and
escherchia
coli
in
broiler
chickens.
Can.
Vet.
J.
19:
187­
193.
Pacific
Environmental
Services,
Inc.
(
PES
Inc.).
1987.
Toxic
air
pollutant/
source
crosswalk
 
a
screening
tool
for
locating
possible
sources
emitting
toxic
air
pollutants.
USEPA
Technical
Report
No.
PB88­
161146.

Pedersen,
F.
and
Selig,
R.
1989.
Predicting
the
consequences
of
short­
term
exposure
to
high
concentrations
of
gaseous
ammonia.
J.
Haz.
Mat.
21:
143­
159.

Phillips,
R.
W.
1970.
Further
clinical
studies
of
trimethylpurin­
6­
Ylammonium
chloride
(
NSC­
51095)
in
malignant
disease
with
special
emphasis
on
cancer
of
urinary
bladder.
Cancer
Chemotherapy
Reports
Part
1
54:
181­
185.

Phillips,
S.
C.
1981.
The
toxicity
to
rat
cerebral
cortex
of
topical
applications
of
acetaldehyde,
ammonia
or
bilirubin.
Neuropathol.
Appl.
Neurobiol.
7:
205­
216.

Poliak,
V.
E.
1981.
Air
pollution
by
ammonia
around
swine­
breeding
complexes.
Gig.
I.
Sanit.
6:
67.
(
Russian).

Powers,
W.
L.,
Terry,
R.
V.,
and
Murphy,
L.
S.
1977.
Fate
of
nitrogen
from
manure
disposal.
In
Proceedings
of
the
National
Conference
on
Disposal
of
Residues
on
Land.
Pp.
156­
160.

Proctor,
N.
H.,
Hughes,
J.
P.,
and
Fischman,
M.
1988.
Chemical
Hazards
in
the
Work
Place.
2nd
edition.
Philadelphia:
I.
B.
Lippinocott
Company.

Propkopieva,
A.
S.
and
Yushkov,
G.
C.
1975.
Toxicology
of
ammonia
in
animals
repeatedly
exposed
for
short
lengths
of
time.
Gig.
Tr.
Prof.
Zabol.
9:
54­
55.
(
Russian).
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Propkopieva,
A.
S.
Yushkov,
G.
C.,
and
Ubasheev,
I.
O.
1973.
Materials
for
a
toxicological
characteristics
of
the
long­
term
effect
of
ammonia
on
the
organisms
of
animal
repeatedly
exposed.
Gig.
Tr.
Prof.
Zabol.
6:
56­
57.
(
Russian).
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Qureshi,
A.
A.,
Flood,
K.
W.,
Thompson,
S.
R.,
Janhurst,
S.
M.,
Iniss,
C.
S.,
and
Rokosh,
D.
A.
1982.
Comparison
of
luminescent
bacterial
test
with
other
bioassays
for
determining
toxicity
of
pure
compounds
and
complex
effluents.
Aquatic
Toxicology
and
Hazard
Assessment:
Fifth
Conference,
ASTM
STP
766.
Pearson,
J.
G.,
Foster,
R.
B.,
and
Bishop,
W.
E.
(
eds.).
American
Society
for
Testing
and
Materials
(
ASTM).
Pp.
179­
195.

Raabe,
W.
and
Lin,
S.
1985.
Pathophysiology
of
ammonia
intoxication.
Exp.
Neurol.
87:
519­
532.
Raabe,
W.
and
Lin,
S.
1984.
Ammonia,
postsynaptic
inhibition
and
CNS­
energy
state.
Brain
Res.
303:
67­
76.
Raabe,
W.
and
Lin,
S.
1983.
Ammonia
intoxication
and
hyperpolarizing
postsynaptic
inhibition.
Exp.
Neurol.
82:
711­
715.

Raabe,
W.
A.
1981.
Ammonia
and
disinhibition
in
cat
motor
cortex
by
ammonium
acetate,
monofluoroacetate
and
insulin­
induced
hypoglycemia.
Bain
Res.
210:
311­
322.

Raabe,
W.
A.
and
Gumnit,
R.
J.
1974.
Disinhibition
in
cat
motor
cortex
by
ammonia.
Pp.
347­
355.

Raabe,
W.
A.
and
Onstad,
G.
R.
1982.
Ammonia
and
methionine
sulfoximine
intoxications.
Brain
Res.
242:
291­
298.

Raj,
P.
K.,
Hagopian,
J.
H.,
Kalelkar,
A.
S.,
and
Cece,
J.
1975.
Predicting
health
hazards
from
ammonia
spills.
Ammonia
Plant
Safety
17:
102­
108.

Rajagopal,
R.
1978.
Impact
of
land
use
on
ground
water
quality
in
the
Grand
Traverse
Bay
region
of
Michigan.
J.
Environ.
Qual.
7:
93­
98.

Rankin,
D.
P.
1979.
The
Influence
of
Un­
ionized
Ammonia
on
the
Long­
term
Survival
of
Sockeye
Salmon
Eggs.
Fisheries
and
Marine
Service
Technical
Report
912.

Reece,
F.
N.,
Lott,
B.
D.,
and
Deaton,
J.
W.
1981.
Low
concentrations
of
ammonia
during
brooding
decrease
broiler
weight.
Poult.
Sci.
60:
937­
940.

Rice,
S.
D.
and
Bailey,
J.
E.
1980.
Survival,
size,
and
emergence
of
pink
salmon,
Oncorhynchus
gorbuscha,
alevins
after
short­
and
long­
term
exposures
to
ammonia.
Fish.
Bull.
78(
3):
641­
648.

Richard,
D.,
Bouley,
G.,
and
Coudene,
C.
1978a.
Effects
of
ammonia
gas
continuously
inhaled
by
rats
and
mice.
Bull
Europ.
Physiopath.
Resp.
14:
573­
582.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Richard,
D.,
Jouany,
J.
M.,
and
Boudene,
C.
1978b.
Acute
toxicity
via
inhalation
route
of
ammonia
gas
in
the
rabbit.
CR
Acad.
Sci.
D.
287:
375­
378.
(
French).

Richardson,
J.
1997.
Acute
ammonia
toxicity
for
eight
New
Zealand
indigenous
freshwater
species.
NZ
J.
Mar.
Freshwater
Res.
31:
185­
190.

Roberge,
A.
and
Charbonneau,
R.
1968.
Metabolism
of
ammonia.
I.
Biochemical
aspect
of
ammonia
intoxication.
Rev.
Can.
Biol.
27(
4):
321­
331.

Roberts,
R.
H.
and
Handman,
S.
E.
1986.
Minimize
ammonia
releases.
Hydrocarbon
Processing
March.
Pp.
58­
64.

Robin,
E.
D.,
Travis,
D.
M.,
Bromberg,
P.
A.,
Forkner,
C.
E.,
and
Tyler,
J.
M.
1959.
Ammonia
excretion
by
mammalian
lung.
Science
129:
270­
271.
Robinette,
H.
R.
1976.
Effect
of
selected
sublethal
levels
of
ammonia
on
the
growth
of
channel
catfish
(
Ictalurus
punctatus).
Prog.
Fish­
Cult.
38(
1):
26­
29.

Rosenfeld,
M.
1932.
Experimental
modification
of
mitosis
by
ammonia.
Arch.
Exp.
Zellforsch.
Gewebezuecht.
14:
1.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Rubin,
A.
J.
and
Elmaraghy,
G.
A.
1977.
Studies
on
the
toxicity
of
ammonia,
nitrate
and
their
mixtures
to
guppy
fry.
Water
Res.
11:
927­
935.

Russo,
R.
C.
and
Thurston,
R.
V.
1977.
The
acute
toxicity
of
nitrite
to
fishes.
In:
Tubb,
R.
A.
(
ed.).
Recent
Advances
in
Fish
Toxicology.
Corvallis:
Corvallis
Environmental
Research
Laboratory.
EPA
600/
3­
77­
085.

Ruyet,
J.
P.
and
Quemener,
H.
C.
1995.
Comparative
acute
ammonia
toxicity
in
marine
fish
and
plasma
ammonia
response.
Aquaculture
136:
181­
194.

Sadasivudu,
B.,
Rao,
T.
I.,
and
Murthy,
C.
R.
1979.
Chronic
metabolic
effects
of
ammonia
in
mouse
brain.
Arch.
Int.
Physiol.
Biochem.
87:
871­
885.

Saifutdinov,
M.
M.
1966.
Maximum
permissible
concentration
of
ammonia
in
the
atmosphere.
Hyg.
Sanit.
31:
171­
176.

Salvatore,
F.,
Bocchini,
V.,
and
Cimino,
F.
1963.
Ammonia
intoxication
and
its
effects
on
brain
an
blood
ammonia
levels.
Biochem.
Pharm.
12:
1­
6.

Saul,
R.
I.
and
Archer,
M.
C.
1984.
Oxidation
of
ammonia
and
hydroxylamine
to
nitrate
in
the
rat
and
in
vitro.
Carcinogen.
5:
77­
81.

Saxena,
P.,
Seigneur,
C.,
and
Pollack,
A.
K.
1986.
A
critical
assessment
of
existing
data
bases
for
acid
deposition
modeling
studies.
J.
Air
Pollut.
Control
Assoc.
36:
48­
54.

Schaerdel,
A.
D.,
White,
W.
J.,
Lang,
C.
M.,
Dvorchik,
B.
H.,
and
Bohner,
K.
1983.
Localized
and
systemic
effects
of
environmental
ammonia
in
rats.
Lab
Anim.
Sci.
33:
40­
45.

Schenker,
S.
and
Warren,
K.
S.
1962.
Effects
of
temperature
variation
on
toxicity
and
metabolism
of
ammonia
in
mice.
J.
Lab
&
Clin.
Med.
60:
291­
301.

Schlesinger,
W.
H.
1991.
Biogeochemistry:
An
Analysis
of
Global
Change.
New
York:
Academic
Press.

Schoeb,
T.
R.,
Davidson,
M.
K.,
and
Linsay,
J.
R.
1982.
Intracage
ammonia
promotes
growth
of
mycoplasma
pulmonis
in
the
respiratory
tract
of
rats.
Infect.
Immum.
38:
212­
217.

Schubauer­
Berigan,
M.
K.,
Monson,
P.
D.,
West,
C.
W.,
and
Ankley,
G.
T.
1995.
Influence
of
pH
on
the
toxicity
of
ammonia
to
Chironomus
tentans
and
Lumbriculus
variegatus.
Environ.
Toxicol.
Chem.
14(
4):
713­
717.
Seegal,
B.
C.
1927.
Chronic
acidosis
in
rabbits
and
in
dogs
with
relation
to
kidney
pathologic
change.
Arch.
Intern.
Med.
39:
550­
563.

Sermon,
B.
A.,
Leung,
P.
M.
B.,
Rogers,
Q.
R.,
and
Glentzen,
D.
W.
1988.
Increase
in
plasma
ammonia
and
amino
acids
when
rats
are
fed
a
44
%
casein
diet.
Physiol.
Behav.
43:
631­
636.

Shah,
K.
D.
1988.
Hazardous
properties
of
ammonia.
Draft.
zitiert
nach:
DSM,
Inventory
of
data
on
chemicals,
Ammonia,
Delft.
1992.
RO471001/
4150J.
In
European
Commission.
1996.
Ammonia.
International
Uniform
Chemical
Information
Database.

Sheehan,
R.
J.
and
Lewis,
W.
M.
1986.
Influence
of
pH
and
ammonia
salts
on
ammonia
toxicity
and
water
balance
in
young
channel
catfish.
Trans.
Amer.
Fish.
Soc.
115:
891­
899.

Shimizu
H.
et
al.
1985.
Jap.
J.
Ind.
Health
27:
400.

Shimkin,
M.
B.,
Lormier,
A.
A.,
Mitchell,
J.
R.,
and
Burroughs,
T.
P.
1954.
Appearance
of
carcinoma
following
single
exposure
to
a
refrigeration
ammonia­
oil
mixture.
Ind.
Hyg.
Occup.
Med.
9:
186­
193.

Sholkovitz,
E.
1973.
Interstitial
water
chemistry
of
the
Santa
Barbara
Basin
sediments.
Geochem.
Cosmochem.
Acta.
37:
2043­
2073.

Silver,
S.
D.
and
McGrath,
F.
P.
1948.
A
comparison
of
acute
toxicities
of
ethylene
imine
and
ammonia
to
mice.
J.
In.
Med.
Toxicol.
30:
7­
9.
In
European
Commission.
1996.
Ammonia.
International
Uniform
Chemical
Information
Database.

Silverman,
L.
and
Whittenberger,
J.
L.
1949.
Blood
changes
due
to
ammonia
inhalation.
Science
109:
121­
122.

Silverman,
L.,
Whittenberger,
J.
L.,
and
Muller,
J.
1949.
Physiological
response
to
man
to
ammonia
in
low
concentrations.
J.
Ind.
Med.
Toxicol.
34:
74­
78.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Sloan,
C.
H.
and
Morie,
G.
P.
1974.
Determination
of
ammonia
in
tobacco
and
tobacco
smoke
with
the
ammonia
electrode.
Anal.
Chem.
Acta.
69:
243­
247.

Slot,
G.
M.
J.
1938.
Ammonia
gas
burns:
an
account
of
six
cases.
Lancet
2:
1356­
57.

Smith,
C.
E.
1974.
Experimental
nitrite
toxicity
in
rainbow
trout
and
chinook
salmon.
Trans.
Amer.
Fish.
Soc.
2:
389­
390.

Smith,
K.
A.
and
Scott,
A.
1983.
Continuous­
Flow
and
discrete
analysis.
In:
Smith,
K.
A.
(
ed.).
Soil
Analysis.
New
York:
Marcel
Dekker,
Inc.
Soderberg,
R.
W.
and
Meade,
J.
W.
1992.
Effects
of
sodium
and
calcium
on
acute
toxicity
of
unionized
ammonia
to
Atlantic
salmon
and
lake
trout.
J.
Appl.
Aquaculture
1(
4):
83­
92.

Soderlund,
R.
and
Svensson,
B.
H.
1976.
The
global
nitrogen
cycle.
In
Svensson,
G.
H.
and
Soderlund,
R.,
(
eds.).
Nitrogen,
Phosphorus,
and
Sulfur:
Global
Sources.
Stockholm.
Pp.
23­
76.

Solbe,
J.
F.
L.
G.
and
Shurben
D.
G.
1989.
Toxicity
of
ammonia
to
early
life
stages
of
rainbow
trout
(
Salmo
gairdneri).
Water
Res.
23(
1):
127­
129.

SRI,
International.
1988.
1988
directory
of
chemical
producers
 
United
States
of
America.
Pp.
457­
468.

Stanley,
M.
and
Andrykovich,
G.
1984.
Living:
An
Introduction
to
Biology.
2nd
ed.
Addison­
Wesley
Pub.
Co.

Stevens,
C.,
Kennaway,
N.
G.,
and
Fellman,
J.
H.
1975.
Ammonia
intoxication:
a
hazard
during
rehabilitation
of
protein­
deprived
rats.
J.
Nutr.
105:
1384­
1390.

Stewart,
B.
A.,
Viets,
F.
G.,
Hutchinson,
G.
L.,
and
Kemper,
W.
D.
1967.
Nitrate
and
other
water
pollutants
under
fields
and
feedlots.
Environ.
Sci.
Tech.
1:
736­
739.

Stoipe,
J.
and
Sedlag,
R.
1976.
Arch.
Exp.
Vet.
Med.
30:
533­
539.
(
German).

Stombaugh,
D.
P.,
Teague,
H.
S.,
and
Roller,
W.
L.
1969.
Effects
of
atmospheric
ammonia
on
the
pig.
J.
Anim.
Sci.
28(
6):
844­
847.

Stoner,
L.
C.
1985.
The
movement
of
solutes
and
water
across
the
vertebrate
distal
nephron.
Renal.
Physiol.
8:
237­
248.

Struzewski,
E.
J.
1971.
Current
status
on
control
and
treatment
of
storm
and
combined
sewer
overflow.
Edison
Water
Quality
Laboratory.

Summerskill,
W.
H.
J.
and
Wolpert,
E.
1970.
Ammonia
metabolism
in
the
gut.
Am.
J.
Clin.
Nutr.
23:
633­
639.

Swaminathan,
B.
V.
and
Jain,
B.
K.
1986.
Safety
in
ammonia
storage
tanks
in
Indian
fertilizer
plants.
Fertilizer
News
31:
35­
36.

Swigert,
J.
P.
and
Spacie,
A.
1983.
Survival
and
Growth
of
Warmwater
Fishes
Exposed
to
Ammonia
Under
Low
Flow
Conditions.
West
Lafayette:
Purdue
University
Water
Resources
Research
Center.

Syracuse
Research
Corporation
(
SRC)
­
LOGKOW
software
V.
1.50
Syracuse
Research
Corporation
(
SRC)
­
WSKOWWIN
V1.17
SysteMed
Corp.
Prepared
for
NASA
and
USAF.
1972.
Toxic
Hazards
Research
Unit
Annual
Technical
Support.

Taiganides,
E.
P.
and
White,
R.
K.
1969.
The
menace
of
noxious
gases
in
animal
units.
Trans.
Am.
Soc.
Agric.
Eng.
12:
359­
362.

Tam,
N.
F.
Y.
and
Wong,
Y.
S.
1996.
Effect
of
ammonia
concentrations
on
growth
of
Chlorella
vulgaris
and
nitrogen
removal
from
media.
Bioresource
Technol.
57:
45­
50.

Targowski,
S.
P.,
Klucinski,
W.,
Babikers,
S.,
and
Nonnecke,
B.
J.
1984.
Effect
of
ammonia
on
in
vivo
and
in
vitro
immune
responses.
Infect.
Immun.
43:
289­
293.
Temple,
P.
J.,
Harper,
D.
S.,
Pearson,
R.
G.,
and
Linzar,
S.
1979.
Toxic
effects
of
ammonia
on
vegetation
in
Ontario.
Environ.
Pollut.
20:
297­
302.

ten
Berge,
W.
F.
1986.
Concentration­
time
mortality
response
relationship
of
irritant
and
systemically
acting
vapours
and
gases.
J.
Haz.
Mat.
13:
301­
309.

Tennessee
Valley
Authority.
1980.
Fertilizer
Trends
1979.
Muscle
Shoals:
National
Fertilizer
Development
Center.

Tepper,
A.,
Constock,
G.
W.,
and
Levine,
M.
1991.
A
longitudinal
study
of
pulmonary
function
in
fire
fighters.
Am.
J.
Ind.
Med.
20:
307­
316.

Tepper,
J.
S.
and
Wood,
R.
W.
1985.
Alterations
in
behavioral
evaluation
of
the
irritating
properties
of
ozone.
Toxicol.
Appl.
Pharmacol.
78:
404­
411.

Terrill,
J.
B.,
Montgomery,
R.
R.,
and
Reinhardt,
C.
F.
1978.
Toxic
gases
from
fires.
Science
200:
1343­
1347.

Thompson,
R.
Y.
and
Halliburton,
I.
W.
1966.
Effect
of
diet
on
the
composition
of
the
kidney.
Biochem.
J.
99:
44.

Thornton,
N.
C.
and
Setterstrom,
C.
1940.
Toxicity
of
ammonia,
chlorine,
hydrogen
cyanide,
hydrogen
sulphide,
and
sulphur,
dioxide
gases.
III.
Green
Plants.
Contrib.
Boyce
Thompson
Inst.
11:
343­
356.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Thurman,
R.
G.,
Kauffman,
F.
C.,
and
Jungerman,
D.
(
eds.).
1986.
Regulation
of
Hepatic
Metabolism:
Intra
and
Intercellular
Compartmentation.

Thurmond,
L.
M.
and
Dean,
J.
H.
1988.
Immunological
responses
following
inhalation
exposure
to
chemical
hazards.
In
Gardner,
D.
E.,
Crapo,
J.
D.,
and
Massaro,
E.
J.
(
eds.).
Toxicology
of
the
Lung.
New
York:
Raven
Press.
Pp.
375­
406.

Thurston,
R.
V.,
Chakoumakos,
C.,
and
Russo,
R.
C.
1981.
Effect
of
fluctuating
exposures
on
the
acute
toxicity
of
ammonia
to
rainbow
trout
(
Salmo
gairdneri)
and
cutthroat
trout
(
S.
clarki).
Water
Res.
15:
911­
917.
Thurston,
R.
V.
and
Russo,
R.
C.
1983.
Acute
toxicity
of
ammonia
to
rainbow
trout.
Trans.
Amer.
Fish
Soc.
112:
696­
704.

Thurston,
R.
V.,
Russo,
R.
C.,
Luedtke,
R.
J.,
Smith,
C.
E.,
Meyn,
E.
L.,
Chakoumakos,
C.
and
Brown,
C.
J.
D.
1984.
Chronic
toxicity
of
ammonia
to
rainbow
trout.
Trans.
Amer.
Fish
Soc.
113:
56­
73.
Thurston,
R.
V.,
Russo,
R.
C.,
and
Phillips,
G.
R.
1983.
Acute
toxicity
of
ammonia
to
fathead
minnows.
Trans.
Amer.
Fish.
Soc.
112:
705­
711.

Thurston,
R.
V.,
Russo,
R.
C.
and
Smith,
C.
E.
1978.
Acute
toxicity
of
ammonia
and
nitrite
to
cutthroat
trout
fry.
Trans.
Amer.
Fish.
Soc.
107(
2):
361­
368.

Tizianello,
A.,
Deferrari,
G.,
Baribotto,
G.,
Robaudo,
C.,
Saffioti,
S.,
Paoletti,
E.,
and
Bruzzone,
M.
1988.
Renal
ammoniagenesis
in
man
with
acute
metabolic
alkalosis.
Contr.
Nephrol.
63:
105­
113.

Tomasso,
J.
R.
and
Carmichael,
G.
J.
1986.
Acute
toxicity
of
ammonia,
nitrite,
and
nitrate
to
the
Guadalupe
bass,
Micropterus
treculi.
Bull.
Environ.
Contam.
Toxicol.
36:
866­
870.

Tomasso,
J.
R.,
Goudie,
C.
A.,
Simco,
B.
A.,
and
Davis,
K.
B.
1980.
Effects
of
environmental
pH
and
calcium
on
ammonia
toxicity
in
channel
catfish.
Trans.
Amer.
Fish.
Soc.
109:
229­
234.

Topping,
D.
C.
and
Visek,
W.
J.
1976.
Nitrogen
intake
and
tumorigenesis
in
rats
injected
with
1,2­
dimethyl.
J.
Nutr.
106:
1583­
1590.

Torda,
C.
1953.
Ammonium
ion
content
and
electrical
activity
of
the
brain
and
during
the
perconvulsive
and
convulsive
phases
induced
by
various
convulsants.
J.
Pharmacol.
Exp.
Ther.
107:
197­
203.

Toth,
B.
1972.
Hydrazine,
methylhydrazine
and
methylhydrazine
sulfate
carcinogenesis
in
Swiss
mice.
Failure
of
ammonium
hydroxide
to
interfere
in
the
development
of
tumors.
Int.
J.
Cancer
9:
109­
118.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Toth,
B.
1969.
Lung
tumor
induction
and
inhibition
of
breast
adenocarcinomas
by
hydrazine
sulfate
in
mice.
J.
Nat.
Cancer
Inst.
42:
469­
475.

Toxic
Release
Inventory
(
TRI)
Industry
Report.
1996.
Nitrogen
fertilizers
(
SIC
2873);
Phosphatic
fertilizers
(
SIC
2874).

Trevisani,
F.,
Bernardi,
M.,
Grilli,
F.,
De
Palma,
R.,
Piazzi,
S.,
Patrono,
D.,
and
Gasbarrini,
G.
1988.
Role
of
gluconeogenesis
from
amino
acids
in
determining
fasting
and
absorptive
levels
of
plasma
ammonia
in
cirrhosis.
Am.
J.
Gastroenterol.
83:
646­
651.

Trussell,
R.
P.
1972.
The
percent
un­
ionized
aqueous
ammonia
solutions
at
different
pH
levels
and
temperatures.
J.
Fish
Res.
Board
Can.
29(
10):
1505­
1507.
Tsujii,
M.,
Kawano,
S.,
Tsuji,
S.,
Ito,
T.,
Nagano,
K.,
Sasaki,
Y.,
Hayashi,
N.,
Fusamoto,
H.,
and
Kamada,
T.
1993.
Cell
kinetics
of
mucosal
atrophy
in
rat
stomach
induced
by
long­
term
administration
of
ammonia.
Gastroenterol.
104:
796­
801.

Underhill,
F.
P.
and
Kapsinow,
R.
1922.
The
comparative
toxicity
of
ammonium
salts.
J.
Biol.
Chem.
54:
451­
457.

U.
S.
Environmental
Protection
Agency
(
USEPA).
1998a.
AP­
42.
Draft.
Vol.
1.
5th
ed.
Research
Triangle
Park:
Office
of
Air
Quality
Planning
and
Standards.

U.
S.
Environmental
Protection
Agency
(
USEPA).
1998b.
Emission
Factor
Documentation
for
AP­
42
Section
9.2.1.
Draft.
Research
Triangle
Park:
Office
of
Air
Quality
Planning
and
Standards.

U.
S.
Environmental
Protection
Agency
(
USEPA).
1998c.
1998
Update
of
Ambient
Water
Quality
Criteria
for
Ammonia.
Washington,
D.
C.:
Office
of
Water.
EPA
822­
R­
98­
008.

U.
S.
Environmental
Protection
Agency
(
USEPA).
1989.
Ambient
Water
Quality
Criteria
for
Ammonia
(
Saltwater)­
1989.
Washington,
D.
C:
Office
of
Water.
EPA
44015­
89­
004
U.
S.
Environmental
Protection
Agency
(
USEPA).
1987a.
Health
Effects
Assessment
for
Ammonia.
Cincinnati.
EPA/
600/
8­
88/
017.

U.
S.
Environmental
Protection
Agency
(
USEPA).
1987b.
National
Air
Toxics
Information
Clearinghouse
(
NATICH)
Data
Base
Report
on
State,
Local
and
EPA
Air
Toxic
Activities.

U.
S.
Environmental
Protection
Agency
(
USEPA).
1987c.
Proposed
Ambient
Water
Quality
Criteria
Document
for
Ammonia
in
Saltwater.
Federal
Register.
Vol.
52,
No.
195.

U.
S.
Environmental
Protection
Agency
(
USEPA).
1986a.
Quality
Criteria
for
Water
 
1986.
Washington,
D.
C.:
Office
of
Water
Regulations
and
Standards.
EPA­
440/
5­
86­
001.

U.
S.
Environmental
Protection
Agency
(
USEPA).
Office
of
Research
and
Development.
1986b.
Summary
Review
for
Health
Effects
Associated
with
Ammonia:
Health
Issue
Assessment.

U.
S.
Environmental
Protection
Agency
(
USEPA).
1985a.
Ambient
Water
Quality
Criteria
for
Ammonia
 
1984.
Washington,
D.
C.:
Office
of
Water
Regulations
and
Standards,
Criteria
and
Standards
Division
EPA­
504/
5­
85­
006.

U.
S.
Environmental
Protection
Agency
(
USEPA).
1985b.
Hazard
Evaluation
Division
Standard
Evaluation
Procedure
Acute
Toxicity
Test
for
Freshwater
Fish.
Washington,
D.
C.
EPA­
540/
9­
85­
006.

U.
S.
Environmental
Protection
Agency
(
USEPA).
1984.
Compilation
of
Air
Pollution
Emission
Factors.
Research
Triangle
Park,
NC.
U.
S.
Environmental
Protection
Agency
(
USEPA).
1981.
Multimedia
Health
Assessment
Document
on
Ammonia.
Cincinnati,
Ohio:
Environmental
Criteria
and
Assessment
Office.
Report
No.
ECAO/
82­
D010.

U.
S.
Environmental
Protection
Agency
(
USEPA).
1981.
Ammonia
Health
Effects.
Ann
Arbor,
MI:
Office
of
Mobile
Source
and
Air
Pollution
Control.
Contract
No.
68­
03­
2928.

U.
S.
Environmental
Protection
Agency
(
USEPA).
1977.
Ammonia.
Research
Triangle
Park:
Office
of
Research
and
Development.
EPA­
600/
1­
77054.

U.
S.
Environmental
Protection
Agency
(
USEPA).
1972.
Ammonia
Study
Using
STORET
Ambient
Monitoring
Data.
Office
of
Water
Planning
and
Standards.

Uzvolgyl,
E.
and
Bojan,
F.
1980.
Possible
in
vivo
formation
of
a
carcinogenic
substance
from
diethlpyrocarbonate
and
ammonia.
J.
Cancer
Res.
Clin.
Oncol.
97:
205­
207.
Valentine,
H.
1964.
A
study
of
the
effect
of
different
ventilation
rates
on
the
ammonia
concentrations
in
the
atmosphere
of
boiler
houses.
Br.
Poult.
Sci.
5:
149­
159.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Verberk,
M.
M.
1977.
Effects
of
ammonia
in
volunteers.
Int.
Arch.
Occup.
Env.
Health
39:
73­
81.

Verschueren,
K.
1996.
Handbook
of
Environmental
Data
and
Organic
Chemicals.
New
York:
John
Wiley
&
Sons,
Inc.

Vezina,
C.
1997.
National
Ammonia
Inventory.
Draft.
Ottawa:
Environment
Canada,
Pollution
Data
Brarnch.
In
Constable,
M.,
Jensen,
F.,
McLernon,
J.
Craig,
G.,
Moore,
D.
1999.
Canadian
Environmental
Protection
Act,
Priority
Substances
List
II:
Supporting
Document
for
Ammonia
in
the
Aquatic
Environment.
Draft,
Unpublished
Version.
Government
of
Canada,
Environment
Canada.

Vines,
H.
M.
and
Wedding,
R.
T.
1960.
Some
effects
of
ammonia
on
plant
metabolism
and
a
possible
mechanism
for
ammonia
toxicity.
Plant
Physiol.
35:
820­
825.

Vinokurova,
M.
K.
and
Mal'kova,
V.
B.
1963.
Toxicology
of
the
selective
herbicide
ammonium
sulfamate.
Gig.
Tr.
Prof.
Zabol.
7:
56­
57.
(
Russian).

Visek,
W.
J.
1984.
Ammonia:
Its
effects
on
biological
systems,
metabolic
hormones
and
reproduction.
J.
diary
Soc.
67:
481­
498.

Visek,
W.
J.
1972.
Effects
of
urea
hydrolysis
on
cell
life­
span
and
metabolism.
Federation
Proceedings.
31(
3):
1178­
1193.

Visek,
W.
J.,
Kolondy,
G.
J.,
and
Gross,
P.
R.
1972.
Ammonia
effects
in
cultures
of
normal
and
transformed
313
cells.
J.
Cell
Physiol.
80:
373.

Walkup,
H.
G.,
and
Nevins,
J.
L.
1966.
The
cost
of
doing
business
in
agricultural
ammonia
for
direct
application.
Agricul.
Ammonia
News
16(
6):
96­
100.

Wallingford,
G.
W.
1977.
Fate
of
nitrogen
from
fertilizer
practices.
In
Disposal
of
residues
on
land.
Rockville:
Information
Transfer,
Inc.
Pp.
152­
155.

Walsh,
L.
M.
1977.
Updating
the
nitrogen
cycle.
In
Disposal
of
residues
on
land.
Rockville,
Maryland:
Information
Transfer,
Inc.

Walton,
M.
1973.
Industrial
ammonia
gassing.
Br.
J.
Ind.
Med.
30:
78­
86.

Warren,
K.
S.
1958.
The
differential
toxicity
of
ammonium
slats.
J.
Clin.
Invest.
37:
497­
501.
In
World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.
Warren,
K.
S.
and
Nathan,
D.
G.
1958.
The
passage
of
ammonia
across
the
blood­
brain
barrier
and
its
relation
to
blood
pH.
J.
Clin.
Invest.
37:
1724­
1728.

Warren
Spring
Laboratory.
1982.
Acidity
of
rainfall
in
the
United
Kingdom.
Wason,
S.,
Stephan,
M.,
and
Breide,
C.
1990.
Ingestion
of
aromatic
ammonia
`
smelling
salts'
capsules.
AJDC.
144:
139­
140.

Waxman,
H.
A.
(
Chairman).
1989.
The
National
Toxic
Release
Inventory
 
Preliminary
Air
toxic
Data.
Subcommittee
on
Health
and
Environment
committee
on
Energy
and
Commerce,
U.
S.
House
of
Representatives.
Washington,
D.
C.

Weatherby,
J.
H.
1952.
Chronic
toxicity
of
ammonia
fumes
by
inhalation.
Proc.
Soc.
Exp.
Biol.
Med.
81:
300­
301.

Weber,
F.
L.,
Friedman,
D.
W.,
and
Fresard,
K.
M.
1998.
Ammonia
production
from
intraluminal
amino
acids
in
canine
jejunum.
Amer.
J.
Physio.
254:
264­
268.

Wehrbein,
G.
F.,
Vipperman,
Jr.,
P.
E.,
Peo,
E.
R.,
and
Cunningham,
P.
J.
1970.
Diammonium
citrate
and
diammonium
phosphate
as
sources
of
dietary
nitrogen
for
growing­
finishing
swine.
J.
Anim.
Sci.
31:
327­
332.

Weiler,
R.
R.
1979.
Rate
of
loss
of
ammonia
from
water
to
the
atmosphere.
J.
Fish
Res.
Board
Can.
36:
685­
689.

Welch,
G.
B.
1969.
Farm
Chem.
122:
31.

West,
Todd,
and
Mason,
Van
Bruggen.
1966.
Textbook
of
Biochemistry.
4th
ed.
New
York:
The
Macmillian
Company.

White,
E.
S.
1971.
A
case
of
near
fatal
ammonia
poisoning.
J.
Occup.
Med.
13:
549­
550.

White,
W.
J.
and
Mans,
A.
M.
1984.
Effect
of
bedding
changes
and
room
ventilation
rates
on
blood
and
brain
ammonia
level
in
normal
rats
and
rats
with
portacaval
shunts.
Lab
Anim.
Sci.
34:
49­
52.

Whitfield,
M.
1974.
The
hydrolysis
of
ammonium
ions
in
sea
water
 
a
theoretical
study.
J.
Mar.
Biol.
Ass.
54:
565­
680.

Wilkin,
D.
C.
and
Flemal,
R.
C.
1980.
Feasibility
of
water
quality
improvement
in
three
Illinois
rivers.
J.
Water
Pollut.
Control
Fed.
52(
2):
293.

Williams,
K.
A.,
Green,
D.
W.,
and
Pascoe,
D.
1986.
Studies
on
the
acute
toxicity
of
pollutants
to
freshwater
macroinvertebrates.
Arch.
Hydrobiol.
106(
1):
61­
70.

Wilson,
R.
P.,
Muhrer,
M.
E.,
and
Bloomfield,
R.
A.
1968.
Comparative
ammonia
toxicity.
Comp.
Biochem.
Physiol.
25:
295­
301.
Windholz,
M.,
Budavari,
S.,
Stroumtsos,
I.
Y.,
and
Fertig,
M.
N.
1976.
Merck
Index.
9th
ed.
Rahway,
New
Jersey:
Merck
and
Co.,
Inc.,
Pp.
69.

Wolaver,
T.
C.
1972.
Distribution
of
Natural
and
Anthropogenic
Elements
and
Levels
in
Precipitation
Across
the
U.
S.:
Theory
and
Quantitative
Models.
Chapel
Hill,
North
Carolina.

Wood,
R.
W.
1981.
Determinants
of
irritant
termination
behavior.
Toxicol.
Appl.
Pharmacol.
61:
260­
268.

World
Health
Organization
(
WHO).
1986.
Ammonia
 
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Yokota,
B.
and
Dantzler.
198.
Comparative
aspects
of
glomerular
filtration.
Renal
Physiol.
8:
194­
300.

Yoshida,
J.,
Nakama,
K.,
and
Nakamura,
R.
1957.
Toxicity
of
urea
and
its
control.
II.
Toxicity
of
ammonium
slats
and
urea
in
rabbits
and
goats.
Nipon
Chikusangaku
Kaiho
28:
185­
191.

Zimmerman,
P.
W.
1949.
Impurities
in
the
air
and
their
influence
on
plant
life.
In
Proceedings
of
the
First
National
Air
Pollution
Symposium.
Pasadean,
California.
Pp.
135­
141.
Zitiert
nach:
DSM,
Inventory
of
data
on
chemicals,
Ammonia,
Delft.
1992.
In
European
Commission.
1996.
Ammonia.
International
Uniform
Chemical
Information
Database.
In
World
Health
Organization
(
WHO).
1986.
Ammonia­
Environmental
Health
Criteria
54.
Geneva:
International
Programme
on
Chemical
Safety.

Zischke,
J.
A.
and
Arthur,
J.
W.
1987.
Effects
of
elevated
ammonia
levels
on
the
fingernail
clam,
Musculium
transversum,
in
outdoor
experimental
streams.
Arch.
Environ.
Contam.
Toxicol.
16:
225­
231.