Document ID: EPA-HQ-OPPT-2003-0067-0011
Agency: epa
Document Type: Supporting & Related Material
Title: 
Posted Date: 2003-11-17T05:00Z

PAGE
INTENTIONALLY
LEFT
BLANK
William
C.
Herz
Director
of
Scientific
Programs
M
E
M
O
R
A
N
D
U
M
TO:
Product
Testing
Recipient
FROM:
William
C.
Herz,
Director,
Scientific
Programs
SUBJECT:
Diammonium
Phosphate
Product
Testing
Dossier
DATE:
March
15,
2003
It
is
with
great
pleasure
that
The
Fertilizer
Institute
(
TFI)
announces
the
completion
and
distribution
of
the
final
product
testing
dossier
for
Diammonium
Phosphate
(
CAS
#
7783­
28­
0).

As
you
are
aware,
TFI
sponsored
this
four
 
year
program
to
develop
and
summarize
screeninglevel
hazard
information
for
high
production
volume
(
HPV)
chemicals.
The
data
elements
generated
represent
a
broad
overview
of
human
health
and
ecological
parameters.
These
include
a
physical
­
chemical
characterization,
environmental
fate,
mammalian
toxicity
and
ecotoxicity.
A
health
and
environmental
safety
data
summary
dossier
was
prepared
for
each
of
the
23
materials.
It
summarizes
the
available
literature
data,
new
testing
data,
category
description
and
read
across
data
as
well
as
provides
a
conclusion
regarding
the
inherent
hazards
of
the
material.

Upon
receipt
of
this
data
a
90
calendar
day
regulatory
trigger
starts
within
which
you
must
update
your
material
safety
data
sheets
(
MS­
DS).
Upon
first
product
shipment
you
must
also
notify
your
distributors
and
employers
once
the
MS­
DS
has
been
updated.
These
regulatory
requirements
are
detailed
in
29
CFR
1910.1200(
g)(
5)
and
29
CFR
1910.1200(
g)(
6)(
i).
The
requirements
are
found
in
the
Occupational
Safety
and
Health
Administration
"
Hazard
Communication
Standard"
(
29
CFR
1910.1200).

Please
note
that
by
receipt
of
this
data;
you
agree
not
to
distribute
or
sell
this
data
beyond
your
own
company.
PAGE
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HEALTH
&
ENVIRONMENTAL
SAFETY
DATA
SUMMARY
DOCUMENT
DIAMMONIUM
PHOSPHATE
(
DAP)
CAS
NO.
7783­
28­
0
Prepared
for:

THE
FERTILIZER
INSTITUTE
January
27,
2003
DRAFT
THE
WEINBERG
GROUP
INC.
1220
Nineteenth
St,
NW,
Suite
300
Washington,
DC
20036­
2400
e­
mail
science@
weinberggroup.
com
WASHINGTON
NEW
YORK
SAN
FRANCISCO
BRUSSELS
PARIS
PAGE
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LEFT
BLANK
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
ii
TABLE
OF
CONTENTS
Page
EXECUTIVE
OVERVIEW..........................................................................................................................
1
SIDS
DATA
PROFILE....................................................................................................................
8
SIDS
DATA
SUMMARY...............................................................................................................
9
1.
GENERAL
INFORMATION...............................................................................................
11
2.
PHYSICAL
CHEMICAL
DATA.........................................................................................
15
3.
ENVIRONMENTAL
FATE
AND
PATHWAYS................................................................
18
4.
ECOTOXICITY....................................................................................................................
19
5.
TOXICITY............................................................................................................................
27
6.
REFERENCES
.....................................................................................................................
34
APPENDICES
Appendix
A
SIDS
Data
Availability
Summary
Appendix
B
SIDS
Data
Summaries
for
the
Phosphate
Compounds
Category:
Diammonium
Phosphate,
Liquid
Polyphosphate,
Monoammonium
Phosphate,
and
Super
Phosphates
ACRONYMS
AND
ABBREVIATIONS
BCF
Bioconcentration
Factor
bw
Body
Weight
DAP
Diammonium
Phosphate
DOT
Department
of
Transportation
FDA
U.
S.
Food
and
Drug
Administration
g/
L
Grams
per
Liter
GLP
Good
Laboratory
Practices
GTSP
Granular
Triple
Super
Phosphate
g/
mL
Grams
per
Milliliter
HSDB
Hazardous
Substance
Data
Bank
IPCS
International
Programme
for
Chemical
Safety
KNO3
Potassium
Nitrate
Koc
Organic
Carbon
Partition
Coefficient
Kow
Octanol/
Water
Partition
Coefficient
LC50
Median
Lethal
Concentration
LD50
Median
Lethal
Dose
LOAEL
Lowest
Observable
Adverse
Effect
Level
LOEC
Lowest
Observable
Effect
Concentration
LOEL
Lowest
Observable
Effect
Level
M
Molar
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
iii
MAP
Monoammonium
Phosphate
mg/
kg
Milligrams
per
Kilogram
mg/
kg/
day
Milligrams
per
Kilogram
per
Day
mg/
L
Milligrams
per
Liter
MHb
Methyl
Hemoglobin
mm
Hg
Millimeters
of
Mercury
mmol/
L
Millimoles
per
Liter
NaNo3
Sodium
Nitrate
NFPA
National
Fire
Prevention
Association
(
NH4)
2NO3
Ammonium
Nitrate
NIOSH
National
Institute
for
Occupational
Safety
and
Health
NO3
Nitrate
NOAEL
No
Observable
Adverse
Effect
Level
NOEC
No
Observable
Effect
Concentration
NOEL
No
Observable
Effect
Level
OECD
Organisation
for
Economic
Co­
operation
and
Development
Pa
Pascal
ppm
Parts
per
Million
SCAS
Semi
Continuous
Activated
Sludge
SIDS
Screening
Information
Data
Set
SSP
Single
Super
Phosphate
TLm
Median
Toxicity
Level
TLV
Threshold
Limit
Value
TFI
The
Fertilizer
Institute
UAN
Urea
Ammonia
Nitrogen
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
1
EXECUTIVE
OVERVIEW
I.
Introduction
The
Fertilizer
Institute,
on
behalf
of
its
member
companies,
initiated
a
Product
Testing
Project
to
collect,
review,
summarize,
and
where
necessary
develop
additional
health
and
environmental
safety
data
for
23
of
its
high
production
volume
inorganic
fertilizer
materials.
These
data
and
summaries
provide
valuable
information
that
can
be
used
to
update
Material
Safety
Data
Sheets,
answer
customers'
questions,
and
support
product
stewardship
efforts.
The
chemical
industry
is
also
participating
in
a
voluntary
program
of
comparable
scope
for
high
production
volume
organic
chemicals.
1
The
23
fertilizer
materials
were
divided
into
five
categories
(
i.
e.,
ammonia
compounds,
nitrate
compounds,
phosphate
compounds,
salts
and
acids)
based
on
their
primary
constituents
as
shown
in
Table
1.
The
use
of
categories
is
a
recognized
and
accepted
method
that
allows
health
and
environmental
safety
data
from
one
chemical
in
the
category
to
be
used
to
represent
one
or
more
other
related
chemicals
in
the
category
(
USEPA
1999).
The
key
is
to
find
similar,
or
at
least
predictable,
patterns
and
trends
among
the
chemicals
in
a
category.
In
this
way,
data
can
be
pooled,
resources
are
optimized,
and
fewer
animals
are
used
in
testing,
all
without
losing
the
ability
to
evaluate
the
hazards
and
safety
of
the
individual
chemicals.
Note
that
some
of
the
materials
fall
into
more
than
one
category
(
e.
g.,
diammonium
phosphate
[
DAP]
is
in
both
the
phosphate
and
ammonia
categories).

Searches
were
conducted
using
on­
line
databases,
standard
scientific
data
compendia,
and
other
published
sources
for
toxicity,
ecotoxicity,
environmental
fate,
and
physical­
chemical
properties.
The
collected
data
were
reviewed
for
quality
and
acceptability
and
then
summarized
according
to
the
Organization
for
Economic
Cooperation
and
Development
(
OECD)
Screening
Information
Data
Set
(
SIDS)
dossier
format
(
OECD
1997).
The
OECD
countries
(
including
the
United
States)
have
agreed
on
a
set
of
tests
and
on
types
of
data
that
are
generally
necessary
to
characterize
the
chemical
behavior
and
potential
hazards
of
chemicals
released
into
the
environment.
The
OECD
SIDS
dossier
was
chosen
as
a
standard
format
for
the
TFI
Product
Testing
Project
in
order
that
it
would
be
scientifically
defensible,
broadly
applicable
and
easily
understandable
to
a
wide
range
of
stakeholders.

The
following
sections
of
this
Executive
Overview
provide
the
rationale
for
development
of
the
Phosphate
Compounds
category
(
Section
II);
a
synopsis
of
the
available
data
related
to
the
physical­
chemical
properties,
environmental
fate,
ecotoxicity
and
toxicity
of
the
phosphate
compounds
in
this
category
(
Section
III);
and
a
conclusion
regarding
the
adequacy
of
the
data
to
sufficiently
characterize
the
category
and
the
need
for
additional
testing
(
Section
IV).

The
data
for
diammonium
phosphate
(
DAP)
are
summarized
in
the
Chemical
Profile
and
Data
Summary
tables.
Because
the
evaluation
of
data
adequacy
relies
on
a
category
approach,
the
Data
Summary
table
references
Appendix
B
when
data
are
available
for
one
or
more
of
the
other
materials
in
the
category
for
those
data
elements
where
no
DAP
data
are
available.
Appendix
B
provides
a
summary
of
the
data
for
all
of
the
phosphate
compounds
in
the
category.
The
1
HPV
Chemical
Challenge
Program;
USEPA
1999
(
http://
www.
epa.
gov/
opptintr/
chemrtk/
volchall.
htm)
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
2
individual
studies
for
DAP
itself
are
presented
in
subsequent
pages
of
this
document.
Separate
data
summary
documents
are
available
for
each
of
the
phosphate
compounds
in
the
category.

II.
Rationale
for
the
Phosphate
Compounds
Category
The
phosphate
compounds
category
for
fertilizer
materials
includes
diammonium
phosphate
(
DAP),
monoammonium
phosphate
(
MAP),
liquid
polyphosphate,
single
superphosphate
(
SSP),
and
granular
triple
superphosphate
(
GTSP)
2.
These
compounds
are
grouped
because
of
chemical
composition
similarities;
phosphate
being
a
primary
component.
All
four
compounds
in
the
category
contain
phosphorus,
hydrogen
and
oxygen.
Three
of
the
compounds
also
contain
nitrogen.
In
additional,
the
four
compounds
have
similar
and
generally
predictable
patterns
of
behavior
in
the
environment
as
well
as
toxicological
properties.

Phosphate
compounds
disassociate
in
water,
and
therefore
may
be
regarded
as
degradable
(
European
Commission
2000a).
They
are
used
by
all
living
organisms
and
both
the
anion
and
cation
are
nutrients
for
algae
(
European
Commission
2000b).
The
primary
contributor
to
aquatic
toxicity
appears
to
be
the
un­
ionized
ammonia
component
of
DAP
and
MAP,
although
excess
phosphate
may
also
have
an
effect
(
Johnson
and
Sanders
1977).
As
a
nutrient,
phosphate
may
also
stimulate
growth
in
algae
(
Aufderheide
and
Bussard
2000).
When
GTSP
is
applied
to
soil,
the
water­
soluble
monocalcium
phosphate
rapidly
dissolves
into
the
soil
solution
and
is
quickly
precipitated
as
available
dicalcium
phosphate.
Inorganic
phosphates
are
generally
retained
by
most
soils
in
fixed
forms
and
therefore
little
is
lost
by
leaching
(
European
Commission
2000a).
Liquid
polyphosphate
fertilizers
behave
essentially
as
salts
in
soil
(
Lohry
2001).

Phosphate
compounds
are
Generally
Recognized
As
Safe
(
GRAS)
by
the
U.
S.
Food
and
Drug
Administration
(
21
CFR
582.1141;
Bhat
and
Ramaswamy
1993)
which
allows
them
to
be
used
as
food
and
color
additives.
Phosphate
is
an
essential
nutrient
required
by
all
organisms
for
proper
metabolism.
Orthophosphates
in
general
are
absorbed
from,
and
to
a
limited
extent
secreted
into,
the
gastrointestinal
tract.
The
transport
of
phosphate
from
the
lumen
of
the
gut
is
an
active,
energy­
dependent
process,
and
there
are
factors
that
appear
to
modify
the
degree
of
its
intestinal
absorption.
Vitamin
D
stimulates
phosphate
absorption,
and
this
effect
has
been
reported
to
precede
the
action
of
the
vitamin
on
transport
of
calcium
ion.
In
general,
in
adults,
about
two
thirds
of
the
ingested
phosphate
is
absorbed
from
the
bowel,
and
that
which
is
absorbed
from
the
gut
is
almost
entirely
excreted
into
the
urine.

2
SSP
and
GTSP
are
combined
into
a
single
Health
and
Environmental
Safety
Data
Summary
Document.
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
3
III.
Summary
of
Data
Available
for
the
Phosphate
Compounds
Category
Detailed
data
summaries
for
phosphate
compounds
are
included
in
subsequent
sections
of
this
Health
and
Environmental
Safety
Data
Summary
Document
for
DAP.
These
data
are
briefly
summarized
here.

Physical­
Chemical
Data
The
density
of
phosphate
compounds
varies
from
0.93­
1.83
g/
L.
All
phosphate
compounds
are
highly
soluble
in
water
with
solubility
ranging
from
18­
588
g/
L
at
temperatures
from
0
to
30
°
C.
The
one
vapor
pressure
value
reported
for
DAP
indicates
low
volatility
(<
100
Pa
at
20
°
C).
No
specific
data
on
the
octanol­
water
partition
coefficient
(
Kow)
are
available,
but
based
on
the
high
water
solubility
it
is
expected
that
Kow
and
bioaccumulation
potential
would
be
low,
although
the
body
does
absorb
phosphates
as
necessary
to
carry
out
vital
functions.
These
compounds
dissociate
and
do
not
undergo
oxidation­
reduction
reactions
so
redox
potentials
are
not
applicable.

Environmental
Fate
and
Pathway
Little
direct
environmental
fate
testing
has
been
conducted
with
the
phosphate
compounds,
largely
because
such
compounds
generally
dissociate
in
solution
and
therefore
photodegradation
and
biodegradation
mechanisms
are
less
important.
However,
phosphates
may
be
regarded
as
biodegradable,
as
they
are
utilized
by
all
living
organisms.
The
phosphorus
cycle
is
well
understood
(
e.
g.
see
www.
ppi
far.
org/
ppiweb/
ppibase.
nsf/$
webindex/
article=
678CA758852569B5005C148685E4A99F).

Ecotoxicity
Acute
96­
hour
studies
have
been
reported
in
the
literature
on
various
fish
species.
These
reported
LC50
values
range
from
90­
5,900
mg/
L
for
DAP
and
other
compounds
in
the
phosphate
category.
Additional,
acute
fish
toxicity
studies
have
been
conducted
for
MAP
and
liquid
polyphosphate
according
to
OECD
protocols
and
under
Good
Laboratory
Practice
(
GLP)
conditions.
Results
indicate
no
toxicity
at
the
highest
nominal
concentration
(
100
mg/
L)
tested
in
both
tests.
MAP
and
liquid
polyphosphate
concentrations
were
calculated
based
on
measurements
of
total
phosphate.
For
the
high
concentration
in
each
test,
the
measured
concentrations
were
85.9
and
101
mg/
L
of
MAP
and
liquid
polyphosphate,
respectively.
Acute
studies
with
Daphnia
magna
were
not
available
for
members
of
the
phosphate
category,
but
studies
have
been
conducted
with
commercial
grade
superphosphate
on
a
similar
daphnid
species,
Daphnia
carinata.
These
72
hour
studies
resulted
in
LC50
values
of
approximately
1,800
mg/
L
at
both
22
and
30
º
C.
Results
from
acute
toxicity
testing
of
other
aquatic
invertebrates
have
also
been
reported,
with
LC50
values
ranging
from
about
1000
to
over
5,000
mg/
L
for
DAP
and
superphosphates.
Based
on
the
standard
Federal
Insecticide
Fungicide
and
Rodenticide
Act
(
FIFRA)
acute
toxicity
ratings
for
fish
and
Daphnia
(
below),
the
compounds
in
this
category
are
considered
slightly
toxic
to
practically
non­
toxic.
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
4
EC/
LC50
(
mg/
L)
Toxicity
Description
<
0.1
Very
Highly
Toxic
0.1­
1
Highly
Toxic
1­
10
Moderately
Toxic
10­
100
Slightly
Toxic
>
100
Practically
Non­
Toxic
Additional
studies
have
been
conducted
to
evaluate
the
toxicity
of
DAP
and
GTSP
to
an
alga
species,
Selenastrum
capricornutum.
Both
materials
were
tested
according
to
OECD
protocols
under
GLP
conditions.
The
resultant
72­
hr
NOECs
were
97.1
and
87.6
mg/
L,
for
DAP
and
GTSP,
respectively.
No
adverse
inhibitory
effects
were
observed
in
either
test.
Some
stimulation
of
growth
was
noted
in
both
tests,
with
resultant
NOEC
values
of
3.57
and
21.6
mg/
L
for
DAP
and
GTSP
respectively.
This
effect
is
consistent
with
the
known
stimulatory
(
nutrient)
effect
of
phosphate
compounds
and
reflects
the
phenomenon
that
some
level
of
phosphate
is
essential
(
e.
g.,
fisheries
productivity
is
dependent
on
sufficient
nutrient
availability)
but
excess
nutrient
levels
can
lead
to
overproduction.

Mammalian
Toxicity
Acute
toxicity
The
only
toxicity
study
available
from
the
literature
was
one
in
which
ewes
were
dosed
with
a
granular
preparation
of
superphosphate.
The
reported
LD50
for
this
study
was
5,000­
6,000
mg/
kg
bw.
To
provide
additional
data
for
the
category,
additional
studies
were
initiated
for
DAP,
MAP,
and
liquid
polyphosphate
using
the
OECD
"
up­
and­
down"
protocol
under
GLP
conditions.
For
all
three
compounds,
rats
received
an
initial
dose
of
2000
mg/
kg
bw
by
oral
gavage.
All
animals
survived,
gained
weight,
and
appeared
active
and
healthy
throughout
the
study
period,
with
no
signs
of
toxicity
observed
for
any
of
the
test
materials.
Similarly,
additional
acute
toxicity
studies
for
exposure
via
the
dermal
route
were
conducted
on
DAP,
MAP,
and
liquid
polyphosphate
using
the
OECD
limit
test
protocol
under
GLP
conditions.
In
dermal
exposures
of
5,000
mg/
kg
bw,
no
signs
of
toxicity
were
observed
and
all
animals
survived,
gained
weight,
and
appeared
active
and
healthy
throughout
the
tests.
Based
on
the
standard
FIFRA
acute
toxicity
ratings
for
mammals
(
below),
the
compounds
in
this
category
are
considered
to
be
of
low
to
very
low
toxicity
(
40
CFR
156.62).

Toxicity
Category
I
II
III
IV
Toxicity
Rating
High
Moderate
Low
Very
Low
Oral
LD50
 
50
mg/
kg
>
50­
500
mg/
kg
>
500­
5000
mg/
kg
>
5000
mg/
kg
Dermal
LC50
 
200
mg/
kg
>
200­
2000
mg/
kg
>
2000­
20,000
mg/
kg
>
20,000
mg/
kg
Inhalation
LC50
 
0.2
mg/
L
>
0.2­
2
mg/
L
>
2­
20
mg/
L
>
20
mg/
L
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
5
Skin
and
eye
irritation
No
specific
animal
studies
are
available
for
skin
and
eye
irritation.
Data
for
the
dermal
studies
described
above
indicate
that
skin
exposures
are
not
a
significant
concern.
However,
dust
from
the
solid
materials
and
concentrated
solutions
of
the
liquid
materials
can
be
mild
irritants.

Genotoxicity
In
vitro
bacterial
and
non­
bacterial
genotoxicity
studies
have
been
conducted
on
DAP
and
GTSP
as
representatives
of
the
category.
Using
the
OECD
bacterial
reverse
mutation
assay
(
Ames)
protocol
under
GLP
conditions,
S.
typhimurium
strains
TA
98,
TA
100,
TA
1535,
TA
1537
were
tested
at
concentrations
of
50,
150,
500,
1,500,
and
5,000
µ
g/
plate.
Using
the
OECD
mammalian
chromosome
aberration
test
under
GLP
conditions,
Chinese
hamster
ovaries
were
tested
at
concentrations
from
185
to
1,480
µ
g/
mL.
In
all
of
the
in
vitro
genotoxicity
studies,
results
were
negative
in
all
cases
both
with
and
without
metabolic
activation.
One
in
vivo
study
is
reported
in
the
literature
from
1988
in
which
single
superphosphate
was
incorporated
into
the
food
of
mice
at
500
mg/
day
for
7
days.
Chromosomal
aberrations
were
observed
in
the
bone
marrow
and
the
authors
concluded
that
single
superphosphate
was
clastogenic
and
possibly
mutagenic.
Given
the
high
doses
of
this
study
and
the
lack
of
effect
noted
in
the
in
vitro
studies
conducted
under
GLP
conditions,
the
genotoxicity
potential
appears
to
be
low.

Repeated
dose,
reproductive
and
developmental
toxicity
Studies
have
been
conducted
on
DAP
and
GTSP
using
the
OECD
Guideline
422
combined
protocol
that
provides
data
on
repeated
dose
exposures
as
well
as
reproductive
and
developmental
endpoints.
Doses
of
250,
750,
and
1500
mg/
kg/
day
were
given
by
gavage
to
male
and
female
rats
daily
(
7
days/
week).
Animals
were
divided
between
separate
toxicity
and
reproductive
subgroups.
The
exposure
period
for
the
toxicity
subgroup
was
35
days,
while
the
exposure
period
for
the
reproductive
subgroup
was
at
most
28
days
among
males
and
53
days
among
females.
No
treatment­
related
mortality
or
signs
of
overt
clinical
toxicity
were
observed
in
either
subgroup.
In
the
toxicity
subgroup,
several
hematological,
clinical
chemistry,
and
histopathological
parameters
among
the
low­,
mid­,
and
high­
dose
animals
showed
effects.
In
the
reproductive
subgroup,
effects
on
body
weight
and
food
consumption
were
observed
in
a
non
dose­
related
manner.
No
effects
on
offspring
were
observed
in
either
study.
For
GTSP,
the
resulting
NOAEL
for
reproductive/
developmental
toxicity
was
750
mg/
kg/
day.
The
resultant
LOAELs
were
250
and
1,500
mg/
kg/
day
for
the
general
toxicity
and
reproductive/
developmental
toxicity,
respectively.
For
DAP,
the
NOAEL
for
general
toxicity
was
250
mg/
kg/
day
and
for
reproductive/
developmental
toxicity
was
1,500
mg/
kg/
day.
The
LOAELs
were
500
and
>
1,500
mg/
kg/
day
for
the
general
toxicity
and
reproductive/
developmental
toxicity,
respectively.

IV.
Conclusion
for
the
Phosphate
Compounds
Category
A
substantial
amount
of
data
has
been
produced
for
the
compounds
in
the
phosphate
compounds
category.
These
data
include
GLP
studies
on
the
acute
oral
and
dermal
toxicity
of
DAP,
MAP,
and
liquid
phosphate
and
bacterial
and
non­
bacterial
genotoxicity
studies
on
DAP
and
GTSP.
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
6
Additionally,
GLP
acute
fish
studies
were
conducted
on
liquid
polyphosphate
and
MAP,
and
GLP
algae
studies
were
conducted
on
DAP
and
GTSP.
Combined
repeat
dose/
reproductive/
developmental
studies
were
also
conducted
on
DAP
and
GTSP.
The
results
indicate
that
these
compounds
are
slightly
toxic
to
practically
non­
toxic
to
aquatic
organisms
and
of
low
to
very
low
toxicity
to
mammals.
The
compounds
in
the
phosphate
category
are
adequately
characterized.
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
7
TABLE
1:
CATEGORIES
FOR
PRODUCT
TESTING
PROJECT
CATEGORY
COMPOUND
CAS
NUMBER
Ammonia
Compounds
Anhydrous
ammonia
Aqua
ammonia
Ammonium
nitrate
Ammonium
sulfate
Ammonium
thiosulfate
Nitrogen
solutions
(
UAN)*
Ammonium
phosphate
sulfate
Diammonium
phosphate
(
DAP)
Monoammonium
phosphate
(
MAP)
Urea
7664­
41­
7
1336­
21­
6
6484­
52­
2
7783­
20­
2
7783­
18­
8
15978­
77­
5
12593­
60­
1
7783­
28­
0
7722­
76­
1
57­
13­
6
Nitrate
Compounds
Sodium
nitrate
Ammonium
nitrate
Potassium
nitrate
Potassium
sodium
nitrate
Nitrogen
solutions
(
UAN)*
7631­
99­
4
6484­
52­
2
7757­
79­
1
7757­
79­
1/
7631­
99­
4
15978­
77­
5
Phosphate
Compounds
Diammonium
phosphate
(
DAP)
Monoammonium
phosphate
(
MAP)
Liquid
polyphosphate
Single
superphosphate**
Granular
triple
superphosphate**
7783­
28­
0
7722­
76­
1
­­
8011­
76­
5
65996­
95­
4
Salts
Potassium
chloride
Potassium
magnesium
sulfate
Potassium
nitrate
Potassium
sodium
nitrate
Potassium
sulfate
Calcium
sulfate
7447­
40­
7
14168­
73­
1
7757­
79­
1
7757­
79­
1/
7631­
99­
4
7778­
80­
5
7778­
18­
9
Acids
Phosphoric
acid
Nitric
acid
Sulfuric
acid
7664­
38­
2
7697­
37­
2
7664­
93­
9
*
=
Nitrogen
solutions
are
represented
largely
by
Urea­
Ammonia­
Nitrogen
(
UAN;
15978­
77­
5)
**
=
Single
superphosphate
and
granular
triple
superphosphate
are
combined
into
a
single
dossier.
­­
=
No
CAS
number
readily
available
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
8
V.
REFERENCES
CITED
21
CFR
582.1141
as
cited
in
Hazardous
Substances
Data
Bank
(
HSDB).
1999.
Calcium
Superphosphate.
National
Library
of
Medicine,
Bethesda,
MD.

40
CFR
156.62.
Toxicity
Category.

Aufderheide,
J.
A.
and
Bussard,
J.
B.
2000.
Toxicity
of
Diammonium
Phosphate
(
DAP)
to
the
Unicellular
Green
Alga,
Selenastrum
Capricornutum.
ABC
Study
No.
46207.

Bhat,
M.
R.
and
Ramaswamy,
C.
1993.
Effect
of
ammonia,
urea
and
diammonium
phosphate
(
DAP)
on
lung
functions
in
fertilizer
plant
workers.
Indian
J.
Physiol.
Pharmacol.
37:
222­
224.

Gilman.
1990.
Pharm
Basis
Therap
8th
Ed.
p.
1501
as
cited
in
HSDB.
1999.
Calcium
Superphosphate.
National
Library
of
Medicine,
Bethesda,
MD.

European
Commission.
2000a.
Superphosphate,
Concd.
International
Uniform
Chemical
Information
Database.
Year
2000
CD
 
ROM
edition.

European
Commission.
2000b.
Diammonium
Hydrogenorthophosphate.
International
Uniform
Chemical
Information
Database.
Year
2000
CD
 
ROM
edition.

Johnson,
W.
W.
and
Sanders,
H.
O.
1977.
Chemical
forest
fire
retardants:
Acute
toxicity
to
five
freshwater
fishes
and
a
scud.
Technical
Papers
of
the
U.
S.
Fish
and
Wildlife
Service.

Lohry,
R.
2001.
Ortho
vs.
poly.
The
Fluid
Journal.
Issue
#
35,
9(
4):
1­
3.

OECD.
1997.
SIDS
Manual.
OECD
Secretariat,
3rd
Revision,
July
1997.

USEPA.
1999.
Development
of
Chemical
Categories
in
the
HPV
Challenge
Program.
www.
epa.
gove/
chemrtk/
catdoc29.
pdf.
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
9
SIDS
DATA
PROFILE
Date:
January
27,
2003
1.01
A.
CAS
No.
7783­
28­
0
1.01
C.
CHEMICAL
NAME
(
OECD
NAME)
Diammonium
phosphate
1.01
D
CAS
DESCRIPTOR
Diammonium
phosphate
1.01
G
STRUCTURAL
FORMULA
(
NH4)
2HPO4
OTHER
CHEMICAL
IDENTITY
INFORMATION
N/
A
1.5
QUANTITY
>
1,000,000
tonnes
1.7
USE
PATTERN
Ammonium
phosphates,
particularly
DAP,
are
the
most
popular
fertilizers
used
worldwide.
Other,
more
limited,
uses
of
DAP
include
textile
processing,
flame
retardants,
laboratory
chemicals,
intermediates,
food
additives,
and
corrosion
inhibitors.
1.9
SOURCES
AND
LEVELS
OF
EXPOSURE
Low
human
exposure
is
expected
because
DAP
is
manufactured
in
closed
systems.
Exposure
to
dusts
and
direct
contact
with
the
material
is
possible
during
fertilizer
application.
ISSUES
FOR
DISCUSSION
The
data
for
diammonium
phosphate
(
DAP)
should
be
evaluated
in
combination
with
the
other
materials
in
the
phosphate
category,
especially
monoammonium
phosphate
(
MAP).
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
10
SIDS
DATA
SUMMARY
Date:
January
27,
2003
DIAMMONIUM
PHOSPHATE
(
CAS
NO.
7783­
28­
0)
SPECIES
PROTOCOL
RESULTS
PHYSICAL­
CHEMICAL
DATA
2.1
Melting
Point
155
°
C
2.2
Boiling
Point
Decomposes
2.3
Density
0.93­
1.619
g/
mL
at
20oC
2.4
Vapor
Pressure
<
100
Pa
at
20
°
C
2.5
Octanol/
Water
Partition
Coefficient
See
Appendix
B*
2.6A
Water
Solubility
588
g/
L
at
20
°
C
2.6B
pH
Value
~
8
2.7
Flash
Point
Not
applicable
2.8
Auto
Flammability
Not
flammable
2.9
Flammability
Not
flammable
2.10
Explosive
Properties
Not
explosive
2.11
Oxidizing
Properties
Not
an
oxidizer
2.12
Oxidation:
Reduction
Potential
See
Appendix
B*
2.13A
Additional
data
See
text
ENVIRONMENTAL
FATE
and
PATHWAY
3.1.1
Photodegradation
No
data
available
3.1.2
Stability
in
Water
Stable
3.1.3
Stability
in
Soil
Stable
3.3.2
Distribution
Calculated,
Fugacity
Level
III
6.5
x
10­
15%
to
air
45.3%
to
water
54.6%
to
soil
0.0755%
to
sediment
3.5
Biodegradation
Phosphorus
cycle
is
well
understood
3.7
Bioaccumulation
Does
not
bioaccumulate
ECOTOXICITY
4.1
Acute
Toxicity
to
Fish
Coho
salmon,
Chinook
salmon,
Rainbow
trout,
Bluegill,
Largemouth
bass,
Tilapia,
Fathead
minnow
96
hrs
LC50
=
90­
1,875
mg/
L
4.2
Acute
Toxicity
to
Aquatic
Invertebrates
Daphnia
magna
Amphipod
Snail,
Worm
96
hrs
96
hrs
See
Appendix
B*
LC50
=
40­
52
mg/
L
LC50
=
1,005­
2,472
mg/
L
4.3
Toxicity
to
Aquatic
Plants
(
Algae)
Selenastrum
capricornutum
72
hrs
NOEC
(
toxicity)
=
97.1
mg
DAP/
L
NOEC
(
stimulation)
=
3.57
mg
DAP/
L
4.4
Toxicity
to
Bacteria
No
data
available
4.5.1
Chronic
Toxicity
to
Fish
No
data
available
4.5.2
Chronic
Toxicity
to
Aquatic
Invertebrates
No
data
available
4.6.1
Toxicity
to
Soil
Dwelling
Organisms
No
data
available
4.6.2
Toxicity
to
Terrestrial
Plants
See
Appendix
B*
4.6.3
Toxicity
to
Other
Non­
Mammalian
Terrestrial
Species
No
data
available
*
See
Appendix
B
results
for
data
on
other
chemicals
in
the
phosphate
category.
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
11
DIAMMONIUM
PHOSPHATE
(
CAS
NO.
7783­
28­
0)
SPECIES
PROTOCOL
RESULTS
TOXICITY
5.1.1
Acute
Oral
Toxicity
Rat
OECD
425
LD50
>
2,000
mg/
kg
bw
5.1.2
Acute
Inhalation
Toxicity
No
data
available
5.1.3
Acute
Dermal
Toxicity
Rat
OECD
402
LD50
>
5000
mg/
kg
bw
5.1.4
Acute
Toxicity,
Other
Routes
No
data
available
5.2.1
Skin
Irritation/
Corrosion
Irritant
5.2.2
Eye
Irritation/
Corrosion
Irritant
5.3
Skin
Sensitization
No
data
available
5.4
Repeated
Dose
Toxicity
Rat
OECD
422
NOAEL
=
250
mg/
kg/
day
5.5
Genetic
Toxicity
in
vitro
.
Gene
mutation
Salmonella
typhimurium
Bacterial
reverse
mutation
assay
(
OECD
471)
Negative
.
Chromosomal
aberration
Chinese
Hamster
Ovaries
Chromosome
aberration
test
(
OECD
473)
Negative
5.6
Genetic
Toxicity
in
vivo
See
Appendix
B*
5.7
Carcinogenicity
No
data
available
5.8
Toxicity
to
Reproduction
Rat
OECD
422
NOAEL
=
1500
mg/
kg/
day
5.9
Developmental
Toxicity/
Teratogenicity
Rat
OECD
422
NOAEL
=
1500
mg/
kg/
day
5.10
Additional
Information
See
text
5.11
Human
Experience
See
text
1.8
Occupational
Exposure
Limits
TLV
as
inert
dust
15
mg/
m3
*
See
Appendix
B
results
for
data
on
other
chemicals
in
the
phosphate
category.
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
12
1.
GENERAL
INFORMATION
1.01
SUBSTANCE
INFORMATION
A.
CAS
number
7783­
28­
0
B.
Name
(
IUPAC
name)
Diammonium
hydrogenorthophosphate
C.
Name
(
OECD
name)
Diammonium
phosphate
(
DAP)

D.
CAS
Descriptor
Diammonium
phosphate
(
DAP)

E.
EINECS
Number
231­
987­
8
F.
Molecular
Formula
H9N2O4P
G.
Structural
Formula
(
NH4)
2HPO4
SMILES:
N(
H)(
H)(
H)(
H)
OP(=
O)(
O)
ON(
H)(
H)(
H)(
H)

H.
Substance
Group
Not
applicable
I.
Substance
Remark
Not
applicable
J.
Molecular
Weight
132.06
1.02
OECD
INFORMATION
A.
Sponsor
Country
Not
applicable
B.
Lead
Organization
Not
applicable
C.
Name
of
Responder
Name:
Mr.
William
C.
Herz,
Director
of
Scientific
Programs
Address/
Phones:
The
Fertilizer
Institute
Union
Center
Plaza
820
First
Street,
NE,
Suite
430
Washington,
DC
20002
USA
Tel:
(
202)
962­
0490
Fax:
(
202)
962­
0577
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
13
1.1
GENERAL
SUBSTANCE
INFORMATION
A.
Type
of
Substance
element
[
];
inorganic
[
X];
natural
substance
[
];
organic
[
];
organometallic
[
];
petroleum
product
[
]

B.
Physical
State
(
at
20
°
C
and
1.013
hPa)

gaseous
[
];
liquid
[
];
solid
[
X]

C.
Purity
>
98%;
pure
DAP
is
21.19%
N,
53.76%
P2O5
1.2
SYNONYMS
Ammonium
phosphate,
dibasic
Diammonium
hydrogenorthophosphate
Diammonium
hydrogenphosphate
Diammonium
phosphate
DAP
Phosphoric
acid,
diammonium
salt
Ammonium
hydrogen
phosphate
1.3
IMPURITIES
Remarks:
None
identified
1.4
ADDITIVES
Remarks:
None
identified
1.5
QUANTITY
(
a)
Remarks:
>
1,000,000
tonnes
References:
European
Commission
2000.
Ammonium
sulfate.
International
Uniform
Chemical
Information
Database.
Year
2000
CD
 
ROM
edition.

(
b)
Remarks:
18,700,000
tonnes
(
1992)
Reference:
United
Nations
Industrial
Development
Organization
(
UNIDO)
and
International
Fertilizer
Development
Center
(
IFDC).
1998.
Fertilizer
Manual.
Kluwer
Academic
Publishers,
The
Netherlands.
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
14
1.6
LABELING
AND
CLASSIFICATION
Remarks:
No
specific
labeling
required.

1.7
USE
PATTERN
A.
General
Type
of
Use:
Category:

(
a)
main
Wide
dispersive
use
industrial
Agricultural
use
Fertilizers
(
b)
main
Wide
and
nondispersive
uses
industrial
Chemical
use
Textile
processing,
flame
retardants,
laboratory
chemicals,
intermediates,
food
additives,
and
corrosive
inhibitors
Remarks:
Ammonium
phosphates,
particularly
DAP,
are
the
most
popular
fertilizers
worldwide
because
of
their
high
analysis
and
good
physical
properties.
Reference:
1)
European
Commission
2000.
Diammonium
Hydrogenorthophosphate.
International
Uniform
Chemical
Information
Database.
Year
2000
CD
 
ROM
edition.
2)
United
Nations
Industrial
Development
Organization
(
UNIDO)
and
International
Fertilizer
Development
Center
(
IFDC).
1998.
Fertilizer
Manual.
Kluwer
Academic
Publishers,
The
Netherlands.

B.
Uses
in
Consumer
Products
Remarks:
DAP
may
be
a
component
of
lawn
and
garden
fertilizers.

1.8
OCCUPATIONAL
EXPOSURE
LIMIT
VALUE
Exposure
limit
value
Type:
OSHA
PEL
Value:
15
mg/
m3
total
dust;
5
mg/
m3
respirable
fraction.
Remark:
No
specific
limit
has
been
set,
however,
the
federal
OSHA
PEL
for
particulates
not
otherwise
regulated
applies
to
all
fertilizer
dusts.
Regulated
limits
may
vary
in
local
jurisdictions.
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
15
1.9
SOURCES
OF
EXPOSURE
Remarks:
Exposures
are
expected
to
be
limited
because
DAP
is
manufactured
in
closed
systems.
DAP
can
be
absorbed
into
the
body
by
inhalation
of
its
aerosol
or
dust,
or
after
direct
exposure
to
skin
or
eyes.
Engineering
controls
such
as
mechanical
ventilation,
process
or
personnel
enclosure,
control
of
process
conditions,
and
process
modifications
significantly
reduce
exposure.
Local
exhaust
(
ventilation)
control
as
close
to
the
point
of
generation
is
both
the
most
economical
and
safest
method
to
minimize
personnel
exposure
to
airborne
concentrations.
Personal
protective
equipment
includes
neoprene
or
nitrile
gloves,
dust
masks
or
fume
hoods
as
appropriate,
chemical
safety
goggles,
and
impervious
apron,
sleeves
and
boots.
Field
exposure
to
workers
is
possible
when
handling
and
using
on
farms
as
a
fertilizer.
However,
fertilizers
are
applied
by
mechanical
spreaders,
which
reduces
contact
exposure.
Environmental
releases
are
controlled
at
the
manufacturing
plant.
Gaseous
emissions
are
low
due
to
use
of
scrubbers
and
liquid
effluent
is
recovered
as
much
as
possible.
Exposure
to
environmental
receptors
is
dependent
on
the
amount
used
as
fertilizer
on
fields,
the
climatic
conditions,
and
the
hydrological
conditions
of
the
area
of
application.
Phosphate
materials
are
common
in
the
environment
and
are
essential
nutrients
for
both
plants
and
animals.
References:
1)
HSDB.
1999.
Diammonium
phosphate.
National
Library
of
Medicine,
Bethesda,
MD.
2)
IPCS.
1993.
International
Chemical
Safety
Cards
No.
0217.
Ammonium
Phosphate
Dibasic.

1.10
ADDITIONAL
REMARKS
A.
Options
for
disposal
Remarks:
DAP
should
be
disposed
of
in
accordance
with
federal,
state
and
local
environmental
control
regulations.
Generally
a
sufficient
method
is
to
sweep
spilled
substance
into
covered
containers,
and
wash
away
the
remainder
with
plenty
of
water.
References:
IPCS.
1993.
International
Chemical
Safety
Cards
No.
0217.
Ammonium
Phosphate
Dibasic.
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
16
2.
PHYSICAL­
CHEMICAL
DATA
2.1
MELTING
POINT
Value:
155
°
C
Decomposition:
Yes
[
X]
No
[
]
Ambiguous
[
]
Sublimation:
Yes
[
]
No
[
X]
Ambiguous
[
]
Method:
Not
specified
GLP:
Yes
[
]
No
[
]
?
[
X]
References:
Lide,
D.
R.
1992.
CRC
Handbook
of
Chemistry
and
Physics.
Boston:
CRC
Press.

2.2
BOILING
POINT
Remarks:
Not
applicable.
Decomposes
References:
European
Commission
2000.
Diammonium
Hydrogenorthophosphate.
International
Uniform
Chemical
Information
Database.
Year
2000
CD
 
ROM
edition.
IPCS.
1993.
International
Chemical
Safety
Cards
No.
0217.
Ammonium
Phosphate
Dibasic.

2.3
DENSITY
(
Relative
Density)

(
a)
Type:
Bulk
density
[
];
Density
[
X];
Relative
Density
[
]
Value:
1.619
g/
mL
Temperature:
20
°
C
Method:
Not
specified
GLP:
Yes
[
]
No
[
]
?
[
X]
References:
Lide,
D.
R.
1992.
CRC
Handbook
of
Chemistry
and
Physics.
Boston:
CRC
Press.

(
b)
Type:
Bulk
density
[
X];
Density
[
];
Relative
Density
[
]
Value:
0.93
g/
mL
Temperature:
20
°
C
Method:
Not
specified
GLP:
Yes
[
]
No
[
]
?
[
]
Remarks:
Granular
product
for
fertilizers
References:
Lewis,
Jr.,
R.
J.
1992.
Sax's
Dangerous
Properties
of
Industrial
Materials.
8th
ed.
Vol.
II.
New
York:
Van
Nostrand
Reinhold.
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
17
2.4
VAPOR
PRESSURE
(
a)
Value:
<
100
Pa
Temperature:
20
°
C
Method:
Not
specified
GLP:
Yes
[
]
No
[
]
?
[
X]
Remarks:
Ammonia
gas
over
granular
fertilizer
product.
References:
European
Commission
2000.
Diammonium
Hydrogenorthophosphate.
International
Uniform
Chemical
Information
Database.
Year
2000
CD
 
ROM
edition.

(
b)
Remarks:
Evaporation
at
20oC
is
negligible.
References:
IPCS.
1993.
International
Chemical
Safety
Cards
No.
0217.
Ammonium
Phosphate
Dibasic.

2.5
PARTITION
COEFFICIENT
logPow
Remarks:
No
specific
data
available.
Based
on
water
solubility
it
is
expected
that
the
log
Pow
would
be
very
low.

2.6
WATER
SOLUBILITY
A.
Solubility
(
a)
Value:
5.88
x
105
mg/
L
Temperature:
20
°
C
Description:
Miscible
[
];
Of
very
high
solubility
[
X];
Of
high
solubility
[
];
Soluble
[
];
Slightly
soluble
[
]
Of
low
solubility
[
];
Of
very
low
solubility
[
];
Not
soluble
[
]
Method:
Not
specified
GLP:
Yes
[
]
No
[
]
?
[
X]
Remarks:
Practically
insoluble
in
ethanol,
acetone,
and
ammonia
References:
Budavari,
S.
1996.
The
Merck
Index.
Whitehouse
Station:
Merck
Research
Laboratories.

B.
pH
Value,
pKa
Value
pH
Value:
~
8
Concentration:
1%
Temperature:
20
°
C
Method:
Not
specified
GLP:
Yes
[
]
No
[
]
?
[
X]
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
18
References:
Budavari,
S.
1996.
The
Merck
Index.
Whitehouse
Station:
Merck
Research
Laboratories.

2.7
FLASH
POINT
(
liquids)

Remarks:
Not
applicable
2.8
AUTO
FLAMMABILITY
(
solid/
gases)

Remarks:
Not
flammable
References:
European
Commission
2000.
Diammonium
Hydrogenorthophosphate.
International
Uniform
Chemical
Information
Database.
Year
2000
CD
 
ROM
edition.

2.9
FLAMMABILITY
Remarks:
Not
flammable
References:
European
Commission
2000.
Diammonium
Hydrogenorthophosphate.
International
Uniform
Chemical
Information
Database.
Year
2000
CD
 
ROM
edition.

2.10
EXPLOSIVE
PROPERTIES
Remarks:
Not
explosive
References:
European
Commission
2000.
Diammonium
Hydrogenorthophosphate.
International
Uniform
Chemical
Information
Database.
Year
2000
CD
 
ROM
edition.

2.11
OXIDIZING
PROPERTIES
Remarks:
No
known
oxidizing
properties
References:
European
Commission
2000.
Diammonium
Hydrogenorthophosphate.
International
Uniform
Chemical
Information
Database.
Year
2000
CD
 
ROM
edition.

2.12
OXIDATION:
REDUCTION
POTENTIAL
Remarks:
No
data
available
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
19
2.13
ADDITIONAL
DATA
Remarks:
The
substance
decomposes
on
heating
above
100oC,
on
contact
with
strong
bases,
producing
toxic
and
corrosive
fumes
including
ammonia,
nitrogen
oxides
and
phosphorous
oxides.
The
solution
in
water
is
a
weak
base.
Reacts
vigorously
with
strong
acids
and
strong
oxidants.
Gradually
gives
off
ammonia
on
exposure
to
air.
References:
IPCS.
1993.
International
Chemical
Safety
Cards
No.
0217.
Ammonium
Phosphate
Dibasic.

3.
ENVIRONMENTAL
FATE
AND
PATHWAYS
3.1
STABILITY
3.1.1
PHOTODEGRADATION
Remarks:
No
data
available
3.1.2
STABILITY
IN
WATER
Remarks:
Can
persist
in
natural
water.
Both
the
anion
and
cation
are
nutrients
for
algae.
References:
European
Commission
2000.
Diammonium
Hydrogenorthophosphate.
International
Uniform
Chemical
Information
Database.
Year
2000
CD
 
ROM
edition.

3.1.3
STABILITY
IN
SOIL
Remarks:
Both
the
anion
and
cation
are
nutrients
for
plants.
References:
European
Commission
2000.
Diammonium
Hydrogenorthophosphate.
International
Uniform
Chemical
Information
Database.
Year
2000
CD
 
ROM
edition.

3.2
MONITORING
DATA
(
ENVIRONMENTAL)

Remarks:
No
data
available.
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
20
3.3
TRANSPORT
AND
DISTRIBUTION
BETWEEN
ENVIRONMENTAL
COMPARTMENTS
INCLUDING
ESTIMATED
ENVIRONMENTAL
CONCENTRATIONS
AND
DISTRIBUTION
PATHWAYS
3.3.1
TRANSPORT
Remarks:
No
data
available.

3.3.2
THEORETICAL
DISTRIBUTION
(
FUGACITY
CALCULATION)

Method:
Fugacity
Level
III
(
Mackay
type)
Mass
Distribution
by
Environmental
Compartment:
Air
6.5
x
10­
15%
Water
45.3%
Soil
54.6%
Sediment
0.0755%
References:
USEPA.
2000.
EPISUITE
Estimation
Program
V.
3.10.
US
Environmental
Protection
Agency.

3.4
IDENTIFICATION
OF
MAIN
MODE
OF
DEGRADABILITY
IN
ACTUAL
USE
Remarks:
No
data
available.

3.5
BIODEGRADATION
Remarks:
The
phosphorus
cycle
is
well
understood.

3.6
BOD5,
COD
OR
RATIO
BOD5/
COD
Remarks:
No
data
available.

3.7
BIOACCUMULATION
Remarks:
Will
not
bioaccumulate.

4.
ECOTOXICITY
4.1
ACUTE/
PROLONGED
TOXICITY
TO
FISH
(
a)
Type
of
test:
Static
[
X];
Semi­
static
[
];
Flow­
through
[
];
Opensystem
[
X];
Closed­
system
[
]
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
21
Species:
Oncorhynchus
kisutch
(
Coho
salmon),
Salmo
gairdneri
(
Rainbow
trout),
Pimephales
promelas
(
Fathead
minnows),
Lepomis
macrochirus
(
Bluegills),
Micropterus
salmoides
(
Largemouth
bass)
Exposure
period:
96
hour
Results:
LC50
(
Coho
salmon
fry;
11oC)
=
90­
580
mg/
L
LC50
(
Coho
salmon
fingerling;
11oC)
=
1,000­>
1,500
mg/
L
LC50
(
Rainbow
trout
fry;
11oC)
=
150­
700
mg/
L
LC50
(
Rainbow
trout
fingerling;
11oC)
=
1,000
mg/
L
LC50
(
Fathead
minnow;
21oC)
=
940­>
1,000
mg/
L
LC50
(
Bluegills;
21oC)
=
>
1,500
mg/
L
LC50
(
Largemouth
bass;
21oC)
=
1,160­>
1,500
mg/
L
Analytical
monitoring:
Yes
[
];
No
[
X];
?
[
]
Method:
Static
tests
were
performed
in
reconstituted
water
with
a
pH
of
7.4.
Fish
were
acclimated
for
at
least
14
days
before
testing.
GLP:
Yes
[
]
No
[
]
?
[
X]
Test
substance:
Chemical
forest
fire
retardant
formulations:
Fire­
Trol
100
and
Fire­
Trol
931
(
ammonium
sulfate
or
polyphosphate
with
an
attapulgite
thickener),
and
Phos­
Chek
202
and
Phos­
Chek
259
(
diammonium
phosphate
with
a
guargum
derivative
thickener)
were
mixed
with
water.
Remarks:
In
all
formulations,
the
toxic
effects
generally
manifested
within
24­
h
of
exposure
and
LC50
values
at
24
and
96
h
were
not
substantially
different.
The
primary
contributor
to
the
acute
toxicity
of
the
compounds
is
believed
to
be
un­
ionized
ammonia.
References:
Johnson,
W.
W.
and
Sanders,
H.
O.
1977.
Chemical
forest
fire
retardants:
Acute
toxicity
to
five
freshwater
fishes
and
a
scud.
Technical
Papers
of
the
U.
S.
Fish
and
Wildlife
Service.

(
b)
Type
of
test:
Static
[
X];
Semi­
static
[
X];
Flow­
through
[
];
Opensystem
[
X];
Closed­
system
[
]
Species:
Sarotherodon
mossambicus
(
Tilapia)
Exposure
period:
96
hour
Results:
LC50
(
pH
7.4;
28oC)
=
550
mg/
L
Analytical
monitoring:
Yes
[
X];
No
[
];
?
[
]
Method:
For
the
mortality
study,
ten
individuals
were
exposed
in
static
systems
to
concentrations
of
DAP
(
100
to
1000
mg/
L).
For
the
food
utilization
and
growth
study,
five
fish
were
exposed
to
DAP
concentrations
ranging
from
200
to
500
mg/
L
These
fish
were
fed
twice
a
day,
and
the
culture
medium
was
changed
every
two
days.
GLP:
Yes
[
]
No
[
]
?
[
X]
Test
substance:
(
NH4)
2HPO4
(
fertilizer
grade
DAP;
NPK
ratio
18:
46:
0)
Remarks:
The
LC50
was
obtained
by
graphical
interpolation
of
mortality
at
different
concentrations.
Feeding
rates
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
22
decreased
with
increasing
concentration
(
LOEC
=
300
mg/
L).
Growth
also
decreased
with
increasing
concentration.
Metabolites
of
DAP
contain
ionized
ammonium
and
phosphate,
and
these
could
disturb
physiological
activities
of
fish
at
high
concentrations.
This
sublethal
concentration
is
40
times
lower
than
that
of
urea.
References:
Palanichamy,
S.,
Arunachalam,
S.,
and
Balasubramanian,
M.
P.
1985.
Food
consumption
of
Sarotherodon
mossambicus
(
Trewaves)
exposed
to
sublethal
concentration
of
diammonium
phosphate.
Hydrobiologia
128:
233­
237.

(
c)
Type
of
test:
Static
[
X];
Semi­
static
[
];
Flow­
through
[
X];
Opensystem
[
X];
Closed­
system
[
]
Species:
Pimephales
promelas
(
Fathead
minnows)
Exposure
period:
96
hour
Results:
LC50
(
DAP;
pH
7.8)
=
155
mg/
L
LC50
(
Phos­
Check
®
202,
7­
8
static)
=
1320
µ
l
PC202/
L
(
equivalent
to
150
mg
DAP/
L)
Analytical
monitoring:
Yes
[
X];
No
[
];
?
[
]
Method:
Concentrations
of
PC202
used
in
both
the
flow
through
and
static
bioassays
were
2500,
1877,
1400,
1050,
and
800
µ
l
PC202/
L
slurry.
Each
20­
L
glass
jar
contained
15­
L
of
solution
and
10
fish.
DAP
toxicity
was
also
measured
directly
in
static
experiments.
GLP:
Yes
[
]
No
[
]
?
[
X]
Test
substance:
DAP
and
Phos­
Check
®
202
(
PC202)
Remarks:
The
Phos­
Check
®
202
slurry
contained
10.6%
DAP
diluted
with
water,
plus
thickeners.
The
flowthrough
LC50
for
PC202
was
1,022
µ
l
PC202/
L.
The
acute
toxicity
of
PC202
is
mainly
due
to
the
toxicity
of
the
DAP
content.
References:
Inman,
R.
C.
1974.
Acute
toxicity
of
Phos­
Check
®
202
and
Diammonium
Phosphate
to
Fathead
Minnows.
U.
S.
Air
Force
Environmental
Health
Laboratory,
Kelly
Air
Force
Base,
TX.
Report
EHL(
K)
74­
31.

(
d)
Type
of
test:
Static
[
X];
Semi­
static
[
];
Flow­
through
[
];
Opensystem
[
X];
Closed­
system
[
]
Species:
Cirrhinus
mrigala
Exposure
period:
96­
hours
Results:
LC50
(
fry;
21
°
C)
=
1,700
mg/
L
LC50
(
fry;
26
°
C)
=
1,610
mg/
L
LC50
(
fry;
31
°
C)
=
298
mg/
L
LC50
(
fry;
36
°
C)
=
115
mg/
L
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
23
LC50
(
fingerlings;
21
°
C)
=
1,875
mg/
L
LC50
(
fingerlings;
26
°
C)
=
1,665
mg/
L
LC50
(
fingerlings;
31
°
C)
=
970
mg/
L
LC50
(
fingerlings;
36
°
C)
=
655
mg/
L
Analytical
monitoring:
Yes
[
];
No
[
];
?
[
X]
Method:
Bioassay
tests
were
conducted
on
fry
and
fingerlings
after
methods
described
in
APHA
(
1985).
Tests
were
made
in
10­
L
aquaria
at
pH
7.5.
Each
concentration
was
tested
in
four
replicates
accompanied
by
controls,
with
ten
fry
and
four
fingerlings
per
aquarium
(
i.
e.,
forty
fry
and
sixteen
fingerlings
per
concentration).
GLP:
Yes
[
]
No
[
]
?
[
X]
Test
substance:
Diammonium
phosphate
((
NH4)
2HPO4),
commercial
grade
Remarks:
The
toxicity
of
DAP
to
fish
increased
with
increasing
water
temperature.
In
addition,
in
tests
in
which
fish
were
exposed
to
both
DAP
and
the
pesticide
simazine,
the
presence
of
DAP
fertilizer
reduced
the
toxicity
of
simazine.
References:
1)
Sarkar,
S.
K.
1997.
Toxicity
of
simazine,
diammonium
phosphate
and
rock
phosphate
to
fish,
snail
and
worm
at
various
water
temperatures.
Geobios
24:
142­
145.
2)
American
Public
Health
Association
(
APHA).
1985.
Standard
Methods
for
the
Examination
of
Water
and
Wastewater.
Washington,
D.
C.

4.2
ACUTE
TOXICITY
TO
AQUATIC
INVERTEBRATES
A.
Daphnia
Remarks:
No
data
available.

B.
Other
aquatic
organisms
(
a)
Type
of
test:
Static
[
X];
Semi­
static
[
];
Flow­
through
[
];
Opensystem
[
X];
Closed­
system
[
]
Species:
Gammarus
pseudolimnaeus
(
amphipod)
Exposure
period:
96­
hours
Results:
LC50
(
Phos­
Chek
202)
=
52
mg/
L
LC50
(
Phos­
Chek
259)
=
40
mg/
L
Analytical
monitoring:
Yes
[
];
No
[
];
?
[
X]
Method:
At
least
10
amphipods
per
concentration
were
exposed
for
96
hours
to
six
concentrations
of
a
commercial
formulation
containing
89%
DAP
plus
thickening,
coloring,
and
anti­
corrosion
additives.
Tests
were
conducted
n
reconstituted
deionized
water
at
a
pH
of
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
24
7.2­
7.5
and
hardness
of
40­
50
mg/
L
as
CaCO3.
Temperature
was
maintained
at
18
±
1oC.
GLP:
Yes
[
]
No
[
]
?
[
X]
Test
substance:
Phos­
Chek
202
and
Phos­
Chek
259
References:
Johnson,
W.
W.
and
Finley,
M.
T.
1980.
Handbook
of
Acute
Toxicity
of
Chemicals
to
Fish
and
Aquatic
Invertebrates.
USDOI,
Fish
&
Wildlife
Service
Resource
Publication
137.

(
b)
Type
of
test:
Static
[
X];
Semi­
static
[
];
Flow­
through
[
];
Opensystem
[
X];
Closed­
system
[
]
Species:
Viviparus
bengalensis
(
snail)
and
Branchiura
sowerbyi
(
worm)
Exposure
period:
96­
hours
Results:
LC50
(
V.
bengalensis;
21
°
C)
=
2,472
mg/
L
LC50
(
V.
bengalensis;
26
°
C)
=
2,450
mg/
L
LC50
(
V.
bengalensis;
31
°
C)
=
1,850
mg/
L
LC50
(
V.
bengalensis;
36
°
C)
=
1,005
mg/
L
LC50
(
B.
sowerbyi;
21
°
C)
=
2,455
mg/
L
LC50
(
B.
sowerbyi;
26
°
C)
=
2,005
mg/
L
LC50
(
B.
sowerbyi;
31
°
C)
=
1,740
mg/
L
LC50
(
B.
sowerbyi;
36
°
C)
=
1,200
mg/
L
Analytical
monitoring:
Yes
[
];
No
[
];
?
[
X]
Method:
Bioassay
tests
were
conducted
on
snails
and
worms
after
methods
described
in
APHA
(
1985).
Tests
were
made
in
500
mL
glass
beakers
at
pH
7.5.
Each
concentration
was
tested
in
four
replicates
accompanied
by
controls,
with
either
six
snails
or
ten
worms
per
beaker
(
i.
e.,
24
snails
and
40
worms
per
concentration).
GLP:
Yes
[
]
No
[
]
?
[
X]
Test
substance:
Diammonium
phosphate
((
NH4)
2HPO4),
commercial
grade
Remarks:
The
toxicity
of
DAP
to
both
species
increased
with
increasing
water
temperature.
In
addition,
in
tests
in
which
snails
and
worms
were
exposed
to
both
DAP
and
the
pesticide
simazine,
the
presence
of
DAP
fertilizer
reduced
the
toxicity
of
simazine.
References:
1)
Sarkar,
S.
K.
1997.
Toxicity
of
simazine,
diammonium
phosphate
and
rock
phosphate
to
fish,
snail
and
worm
at
various
water
temperatures.
Geobios
24:
142­
145.
2)
American
Public
Health
Association
(
APHA).
1985.
Standard
Methods
for
the
Examination
of
Water
and
Wastewater.
Washington,
D.
C.
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
25
4.3
TOXICITY
TO
AQUATIC
PLANTS,
e.
g.
algae
Species:
Selenastrum
capricornutum
Endpoint:
Biomass
[
X
];
Growth
rate
[
X
];
Other
[
]
Exposure
period:
72
hours
Results:
NOEC
(
toxicity)
=
97.1
mg
DAP/
L
NOEC
(
stimulation)
=
3.57
mg
DAP/
L
Analytical
monitoring:
Yes
[
X
];
No
[
];
?
[
]
Method:
OECD
201,
1984.
Open­
system
[
X
];
Closed­
system
[
]
GLP:
Yes
[
X
]
No
[
]
?
[
]
Test
substance:
Diammonium
phosphate
(
7783­
28­
0)
Remarks:
No
adverse
inhibitory
effects
were
observed.
There
was
some
stimulation
at
6.25
mg/
L
and
above.
This
was
consistent
with
the
known
stimulatory
effect
of
phosphate
compounds.
The
reported
NOEC
reflects
this
stimulatory
response
and
is
not
considered
to
be
an
adverse
effect.
All
reported
concentrations
were
calculated
from
the
total
phosphate
measurements.
References:
Aufderheide,
J.
A.
and
Bussard,
J.
B.
2000.
Toxicity
of
Diammonium
Phosphate
(
DAP)
to
the
Unicellular
Green
Alga,
Selenastrum
capricornutum.
ABC
Study
No.
46207.

4.4
TOXICITY
TO
BACTERIA
Remarks:
No
data
available.

4.5
CHRONIC
TOXICITY
TO
AQUATIC
ORGANISMS
4.5.1
CHRONIC
TOXICITY
TO
FISH
Remarks:
No
data
available.

4.5.2
CHRONIC
TOXICITY
TO
AQUATIC
INVERTEBRATES
Remarks:
No
data
available.

4.6
TOXICITY
TO
TERRESTRIAL
ORGANISMS
4.6.1
TOXICITY
TO
SOIL
DWELLING
ORGANISMS
Remarks:
No
data
available.
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
26
4.6.2
TOXICITY
TO
TERRESTRIAL
PLANTS
Remarks:
No
data
available.

4.6.3
TOXICITY
TO
OTHER
NON
MAMMALIAN
TERRESTRIAL
SPECIES
(
INCLUDING
AVIAN)

Remarks:
No
data
available.

4.7
BIOLOGICAL
EFFECTS
MONITORING
(
INCLUDING
BIOMAGNIFICATION)

Remarks:
No
data
available.

4.8
BIOTRANSFORMATION
AND
KINETICS
Remarks:
No
data
available.

4.9
ADDITIONAL
REMARKS
(
a)
Type
of
test:
Static
[
];
Semi­
static
[
];
Flow­
through
[
X];
Other
(
e.
g.
field
test)
[
]
Open­
system
[
];
Closed­
system
[
]
Species:
Clarias
batrachus
Exposure
period:
144­
h
Results:
See
remarks.
Analytical
monitoring:
Yes
[
X];
No
[
];
?
[
]
Method:
Healthy
fish
collected
from
the
river
Gomti
of
Lucknow
(
India)
were
exposed
to
0,
810,
860,
920,
970,
1010,
or
1100
mg
DAP/
L.
The
pH
varied
between
7.05
and
7.57
and
the
temperature
varied
between
23.1
and
25.1oC.
Blood
was
collected
and
analyzed
for
hemoglobin,
RBC
count,
Hct,
and
TLC.
GLP:
Yes
[
]
No
[
]
?
[
X]
Test
substance:
Diammonium
phosphate
((
NH4)
2HPO4)
Remarks:
Hemoglobin
content
decreased
at
all
concentrations.
Hemoglobin
was
minimum
at
highest
exposures,
which
proved
lethal
to
fish.
At
all
concentrations,
the
RBC
count
was
lowest
at
the
144­
h
exposure.
At
higher
concentrations
the
cell
count
decreased
suddenly.
The
hematocrit
content
decreased
over
long
durations,
but
seemed
unaffected
at
lower
concentrations
and
during
shorter
intervals.
Similar
results
were
seen
with
another
fish
species,
Heteropneutes
fossilis.
Effects
of
DAP
were
more
pronounced
than
urea.
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
27
References:
1)
Trivedi,
S.
P.,
Singh,
P.,
Sethi,
N.
and
Singh,
R.
K.
1990.
Evaluations
of
hematotoxic
effects
of
two
commonly
used
fertilizers,
diammonium
phosphate
and
urea,
on
fish
Clarias
batrachus.
Ecotoxicology
and
Environmental
Safety.
19:
135­
142.
2)
Trivedi,
S.
P.,
Sethi,
N.,
Singh,
S.
K.
and
Singh,
P.
1989.
Impact
of
fertilizer,
diammonium
phosphate,
on
haematological
profile
of
teleost
fish,
Heteropneutes
fossilis.
Bio.
Mem.
15:
166­
174.

(
b)
Type
of
test:
Static
[
X];
Semi­
static
[
];
Flow­
through
[
];
Other
(
e.
g.
field
test)
[
]
Open­
system
[
X];
Closed­
system
[
]
Species:
Clarias
batrachus
(
teleost)
Exposure
period:
144­
hours
Results:
See
remarks.
Analytical
monitoring:
Yes
[
];
No
[
];
?
[
X]
Method:
Fish
were
collected
from
river
Gomti
of
District
Lucknow
(
India)
and
were
exposed
to
six
concentrations
of
diammonium
phosphate
ranging
from
810
to
1100
mg/
L.
After
144­
hours,
fish
were
sacrificed
and
their
liver,
kidney,
and
muscle
tissues
examined.
GLP:
Yes
[
]
No
[
]
?
[
X]
Test
substance:
Diammonium
phosphate
((
NH4)
2HPO4)
Remarks:
Peak
elevations
in
5'­
Nucleotidase
levels
were
seen
after
120­
hours
exposure
at
860
mg/
L
in
liver
and
kidney
and
after
144­
hours
at
810
mg/
L
in
muscles.
Peak
inhibition
was
observed
after
24­
hours
at
1010
mg/
L
in
liver
and
at
1100
mg/
L
in
kidney
and
muscle
tissue.
The
authors
suggest
that
diammonium
phosphate
may
result
in
disturbances
in
the
general
metabolism
of
fish
by
altering
the
activity
of
5'­
Nucleotidase.
The
significance
of
this
biomarker
of
high
dose
exposure
to
actual
toxicity
at
environmentally
relevant
doses
is
uncertain.
References:
Naqvi,
T.
S.,
Naqvi.
M.
S.,
and
Singh,
R.
K.
1993.
Effect
of
fertilizer
diammonium
phosphate
on
liver,
kidney
and
muscle
5'­
nucleotidase
activity
of
fresh
water
teleost
fish
Clarias
batrachus.
Biomedical
and
Environmental
Sciences
6:
385­
388.

(
c)
Type
of
test:
Static
[
X];
Semi­
static
[
];
Flow­
through
[
];
Other
(
e.
g.
field
test)
[
]
Open­
system
[
X];
Closed­
system
[
]
Species:
Clarias
batrachus
(
teleost)
Endpoint:
Length
of
fish
[
];
Weight
of
fish
[
];
Reproduction
rate
[
];
Other
[
X]
Exposure
period:
24­
to
144­
hours
Results:
See
remarks.
Analytical
monitoring:
Yes
[
];
No
[
];
?
[
X]
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
28
Method:
Healthy
fish
collected
from
the
river
Gomti
at
Lucknow
(
India)
were
acclimatized
for
96­
hours.
Aquaria
were
maintained
at
28
°
C
and
pH
7.22.
Fish
were
exposed
for
24,
48,
72,
96,
120
or
144­
hours
to
810,
860,
920,
970,
1010,
and
1100
mg/
L
diammonium
phosphate.
Each
treatment
group
consisted
of
ten
fish.
Blood
was
collected
from
the
tail
to
determine
levels
of
serum
glutamate
pyruvate
transaminase.
GLP:
Yes
[
]
No
[
]
?
[
X]
Test
substance:
Diammonium
phosphate
((
NH4)
2HPO4).
Remarks:
The
enzymatic
activity
increased
above
control
in
the
initial
periods
of
exposures
at
all
concentrations,
except
at
the
highest
concentration,
which
proved
lethal
and
reduced
activity.
While
the
authors
conclude
that
DAP
causes
toxicity
in
the
liver
of
C.
batrachus,
this
endpoint
really
is
a
biomarker
for
exposure.
The
true
toxicological
significance
of
this
high
dose
response
relative
to
environmentally
significant
doses
and
endpoints
is
uncertain.
References:
Singh,
R.,
Singh,
R.
K.,
and
Pande,
P.
N.
1995.
Biochemical
toxicity
of
fertilizer
di­
ammonium
phosphate
to
fish
Clarias
batrachus.
Uttar
Pradesh
J.
Zool.
15(
2):
144­
148.

5.
TOXICITY
5.1
ACUTE
TOXICITY
5.1.1
ACUTE
ORAL
TOXICITY
Type:
LD0
[
];
LD100
[
];
LD50
[
X
];
LDL0
[
];
Other
[
]
Species/
strain:
Sprague­
Dawley
rat,
albino
Value:
>
2,000
mg/
kg
bw
Method:
OECD
425,
"
Up­
and­
Down
Protocol"
An
initial
dose
of
two
thousand
milligrams
of
the
test
substance
per
kilogram
of
bodyweight
was
administered
to
one
healthy
female
rat
by
oral
gavage.
Due
to
the
absence
of
mortality
in
this
animal
a
second
female
received
the
same
dose
level.
Following
the
completion
of
dosing
and
100%
survival
in
a
total
of
three
females,
a
group
of
three
males
was
tested
(
simultaneously)
at
the
above
dose
level.
All
animals
were
observed
for
mortality,
signs
of
gross
toxicity,
and
behavioral
changes
at
least
once
daily
for
14
days
after
dosing.
Bodyweights
were
recorded
prior
to
administration
and
again
on
Days
7
and
14
(
termination)
after
dosing.
Necropsies
were
performed
on
all
animals
at
terminal
sacrifice.
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
29
GLP:
Yes
[
X
]
No
[
]
?
[
]
Test
substance:
Diammonium
phosphate
(
dark
brown
granules)
Remarks:
Prior
to
use,
the
test
substance
was
ground
in
a
coffee
mill
and
administered
by
gavage
as
a
70%
w/
w
suspension
in
distilled
water
(
preliminary
solubility
testing
indicated
that
suspension
in
excess
of
70%
were
too
viscous
to
be
administered
properly).
All
animals
survived,
gained
weight
and
appeared
active
and
healthy
throughout
the
study
period.
No
signs
of
toxicity
were
observed.
References:
Merkel,
D.
J.
2000.
Acute
oral
toxicity:
Up
and
down
procedure
in
rats­
limit
test.
Product
Safety
Labs
study
number
9733.

5.1.2
ACUTE
INHALATION
TOXICITY
Remarks:
No
data
available.

5.1.3
ACUTE
DERMAL
TOXICITY
Type:
LD0
[
];
LD100
[
];
LD50
[
X
];
LDL0
[
];
Other
[
]
Species/
strain:
Sprague­
Dawley
rat,
albino
Value:
>
5,000
mg/
kg
bw
Method:
OECD
402,
1987.
Limit
test
(
5
males/
5
females).
DAP
was
applied
to
the
shaved
intact
skin
on
the
back
of
each
rat
and
covered
with
gauze
pads.
After
24
hours
the
pads
were
removed
and
the
test
areas
gently
wiped
with
water.
GLP:
Yes
[
X
]
No
[
]
?
[
]
Test
substance:
Diammonium
phosphate
(
dark
brown
granules)
Remarks:
Prior
to
application,
the
test
substance
was
ground
in
a
coffee
mill
and
moistened
to
achieve
a
dry
paste
by
preparing
a
85%
w/
w
mixture.
All
animals
survived,
gained
weight
and
appeared
active
and
healthy
throughout
the
test.
No
signs
of
toxicity
were
observed.
References:
Merkel,
D.
J.
2000.
Acute
dermal
toxicity
study
in
ratslimit
test.
Product
Safety
Labs
study
number
9736.

5.1.4
ACUTE
TOXICITY
BY
OTHER
ROUTES
OF
ADMINISTRATION
Remarks:
No
data
available.
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
30
5.2
CORROSIVENESS/
IRRITATION
5.2.1
SKIN
IRRITATION/
CORROSION
Remarks:
Irritant
References:
European
Commission
2000.
Diammonium
Hydrogenorthophosphate.
International
Uniform
Chemical
Information
Database.
Year
2000
CD
 
ROM
edition.

5.2.2
EYE
IRRITATION/
CORROSION
Remarks:
Irritant
References:
European
Commission
2000.
Diammonium
Hydrogenorthophosphate.
International
Uniform
Chemical
Information
Database.
Year
2000
CD
 
ROM
edition.

5.3
SKIN
SENSITIZATION
Remarks:
No
data
available.

5.4
REPEATED
DOSE
TOXICITY
Species:
Rat
Sex/
strain:
Male/
Female
Crl:
CD(
SD)
IGS
BR
Route
of
administration:
Gavage
Exposure
period:
Animals
were
divided
between
two
subgroups
(
toxicity
and
reproductive
subgroups).
The
exposure
period
for
the
toxicity
subgroup
was
35
days,
while
the
exposure
period
for
the
reproductive
subgroup
was
at
most
28
days
among
male
and
53
days
among
females.
Frequency
of
treatment:
Daily
Doses:
0,
250,
750,
and
1,500
mg/
kg/
day
(
doses
were
selected
based
on
parameters
assessed
in
a
range­
finding
study
at
concentrations
up
to
1,000
mg/
kg/
day)
NOAEL:
250
mg/
kg/
day
(
general
toxicity)
1,500
mg/
kg/
day
(
reproduction/
developmental
toxicity)
LOAEL:
500
mg/
kg/
day
(
general
toxicity)
>
1,500
mg/
kg/
day
(
reproduction/
developmental
toxicity)
GLP:
Yes
Method:
Toxicity
subgroup:
Animals
comprising
the
toxicity
subgroup
(
5
males
and
5
females
per
dose
group)
were
administered
Diammonium
Phosphate
(
DAP)
for
5
weeks
(
7
days/
week)
via
gavage
administration.
Among
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
31
toxicity
subgroup
animals,
functional
observations
(
sensory
reactivity,
grip
strength,
motor
activity)
and
bleeds
for
hematology
and
blood
chemistry
were
conducted
during
week
5
of
treatment.
Organ
weights
were
recorded
at
termination
during
Week
6
and
major
organs
and
tissues
(
and
any
other
abnormalities
observed
at
necropsy)
were
processed
for
microscopic
examination.
Reproductive
subgroup:
Animals
comprising
the
reproductive
subgroup
(
5
males
and
10
females)
were
administered
DAP
for
a
period
encompassing
approximately
53
continuous
days
via
oral
gavage:
14
days
of
initial
treatment,
plus
a
maximum
of
14
days
of
cohabitation
to
ensure
mating,
and
among
females,
at
least
25
days
to
litter
and
rear
their
young
until
day
4
of
age.
Histology
for
reproductive
subgroup
animals
was
restricted
to
retained
reproductive
organs
(
and
any
other
abnormalities
observed
at
necropsy).
Result:
Toxicity
subgroup:
There
were
no
treatment­
related
deaths
and
no
signs
of
overt
clinical
toxicity.
Bodyweight
gain
and
food
consumption
appeared
to
be
suppressed
among
males
at
1,500
mg/
kg/
day.
Bodyweight
gain
for
reproductive
subgroup
females
receiving
1,500
mg/
kg/
day
was
reduced
during
the
first
week
of
gestation,
after
which
they
returned
to
levels
comparable
to
the
controls
for
the
remainder
of
the
study.
The
only
treatment­
related
change
evident
in
haematology
was
a
reduction
in
activated
partial
thromboplastin
time
for
males
at
750
or
1,500
mg/
kg/
day.
There
was
a
slightly
greater
variability
in
blood
chemistry
parameters,
and
the
following
were
blood
chemistry
changes
for
males
that
may
represent
an
effect
of
treatment:
a
non
dosage­
dependant
elevation
of
alkaline
phosphatase
levels
at
750
and
1,500
mg/
kg/
day;
reduced
glucose
and
phosphorous
levels
at
1,500
mg/
kg/
day;
a
dosage­
dependant
reduction
in
total
protein
at
750
and
1,500
mg/
kg/
day
with
a
slightly
elevated
albumin/
globulin
ratio
at
the
high
dose.
Changes
in
females
were
limited
to
a
decrease
in
phosphorous
levels
and
a
non­
significant
increase
in
alkaline
phosphatase
levels
at
1,500
mg/
kg/
day.
Reddening
of
the
extremities
during
the
first
week
of
dosing
was
observed
in
all
doses,
including
250
mg/
kg/
day
but
reduced
as
the
treatment
period
progressed.
Histological
examination
of
the
stomachs
revealed
some
submucosal
inflammation
at
all
doses,
but
this
change
was
not
dose
dependent
and
was
not
statistically
significant
at
the
low
dose.
Reproductive
subgroup:
There
were
no
treatment­
related
deaths
and
no
signs
of
overt
clinical
toxicity.
Mating
performance
and
fertility
were
unaffected
by
treatment,
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
32
and
parental
treatment
had
no
apparent
effect
on
the
offspring
to
day
4
of
age.
Test
substance:
Diammonium
Phosphate
(
DAP)
Reference:
Huntingdon
Life
Sciences
Ltd.
2002.
DAP:
4­
Week
General
Toxicity
and
Reproductive/
Developmental
Toxicity
Screening
Test.
Study
Number
WBG
001/
022948.

5.5
GENETIC
TOXICITY
IN
VITRO
A.
Bacterial
Test
Type:
Bacterial
reverse
mutation
assay
System
of
testing:
Species/
strain:
Salmonella
typhimurium
TA
98,
TA100,
TA1535,
TA1537
and
E.
coli
WP2
uvrA
Concentration:
50,
150,
500,
1,500,
and
5,000
µ
g/
plate
Metabolic
activation:
With
[
];
Without
[
];
With
and
Without
[
X];
Results:
Cytotoxicity
conc:
With
metabolic
activation:
None
observed
Without
metabolic
activation:
None
observed
Precipitation
conc:
5,000
µ
g/
plate
Genotoxic
effects:
+
?
­
With
metabolic
activation:
[
]
[
]
[
X]
Without
metabolic
activation:
[
]
[
]
[
X]
Method:
OECD
Guideline
471
(
Ninth
Addendum;
February
1998)
GLP:
Yes
[
X]
No
[
]
?
[
]
Test
substance:
Diammonium
phosphate
(
DAP),
purity:
100%
Remarks:
Concentrations
from
10
to
50
mg/
mL
of
the
test
article
in
water
were
workable
suspensions.
References:
Wagner,
V.
O.
and
Klug,
M.
L.
2001.
Bacterial
Reverse
Mutation
Assay:
Diammonium
Phosphate
(
DAP).
BioReliance.
Sponsor
Project
Number
TFI002­
AMES.

B.
Non­
bacterial
in
vitro
test
Type:
Mammalian
chromosome
aberration
test
System
of
testing:
Species/
strain:
Chinese
hamster
ovaries
Concentration:
185
to
1,480
µ
g/
mL
Metabolic
activation:
With
[
];
Without
[
];
With
and
Without
[
X];
Results:
Cytotoxicity
conc:
With
metabolic
activation:
None
Without
metabolic
activation:
None
Precipitation
conc:
None
Genotoxic
effects:
+
?
­
With
metabolic
activation:
[
]
[
]
[
X]
Without
metabolic
activation:
[
]
[
]
[
X]
Method:
OECD
Guideline
473
(
Ninth
Addendum;
February
1998)
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
33
GLP:
Yes
[
X]
No
[
]
?
[
]
Test
substance:
Diammonium
phosphate
(
DAP),
purity:
100%
References:
Gudi,
R.
and
Brown,
C.
2001.
In
Vitro
Mammalian
Chromosome
Aberration
Test:
Diammonium
Phosphate
(
DAP).
BioReliance.
Sponsor
Project
Number
TFI002­
CHO.

5.6
GENETIC
TOXICITY
IN
VIVO
Remarks:
No
data
available.

5.7
CARCINOGENICITY
Remarks:
No
data
available.

5.8
TOXICITY
TO
REPRODUCTION
Remarks:
See
summary
in
Section
5.4
5.9
DEVELOPMENTAL
TOXICITY/
TERATOGENICITY
Remarks:
See
summary
in
Section
5.4
5.10
OTHER
RELEVANT
INFORMATION
Remarks:
Toxicity
of
diammonium
phosphate
is
considered
to
be
"
low
to
moderate".
It
is
an
eye,
respiratory
and
skin
irritant,
and
appears
to
be
stronger
in
these
respects
than
monoammonium
phosphate.
No
evidence
was
found
for
chronic
effects
distinct
from
acute
toxicity.
A
number
of
instances
of
livestock
poisoning
have
been
reported
due
to
accidents
in
the
handling
of
feeds
and
fertilizer
fortified
with
ammonium
salts,
including
DAP.
Reference:
European
Commission
2000.
Diammonium
Hydrogenorthophosphate.
International
Uniform
Chemical
Information
Database.
Year
2000
CD
 
ROM
edition
.
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
34
5.11
EXPERIENCE
WITH
HUMAN
EXPOSURE
(
a)
Remarks:
DAP
is
affirmed
as
"
generally
recognized
as
safe"
(
GRAS)
for
use
as
a
food
additive
for
both
human
food
and
ruminant
feed
according
to
prescribed
conditions.
Reference:
21
CFR
582.1141
as
cited
in
Hazardous
Substances
Data
Bank
(
HSDB).

(
b)
Results:
Urea
plant
workers
showed
significant
reduction
in
Peak
Expiratory
Flow
Rate
per
minute
(
PEFR/
min),
but
not
Forced
Vital
Capacity
(
FVC)
and
Forced
Expiratory
Volume
(
FEV),
when
compared
to
the
controls.
Ammonia
plant
workers
showed
a
decrease
in
FEV,
and
FEFR/
min,
but
not
FVC.
DAP
plant
workers
showed
a
significant
reduction
in
all
three
parameters.
All
three
fertilizer
chemicals
appear
to
affect
larger
airways
immediately.
According
to
the
authors,
the
volatility
of
ammonia
and
DAP
enable
them
to
enter
the
smaller
bronchioles
and
cause
hypersensitive
reaction
leading
to
bronchospasm.
These
changes
apparently
occur
slowly
and
continuously.
These
fertilizers
also
affect
the
pulmonary
alveoli
only
after
longer
exposure
and
cause
restrictive
types
of
lung
disorders.
Of
the
three
materials,
DAP
has
the
greatest
affect,
followed
by
ammonia,
and
urea
has
the
least
affect.
Remarks:
Lung
function
tests
were
performed
on
30
diammonium
phosphate,
31
ammonia,
and
30
urea
plant
workers
at
the
Mangalore
Fertilizer
Chemical
Plant.
A
group
of
68
people
served
as
controls.
All
tests
were
performed
in
the
standing
position,
exhaling
into
a
spirometer.
Each
test
was
repeated
three
times
and
the
highest
reading
was
taken
for
calculation.
Subjects
were
split
into
two
groups
of
up
to
10­
years
of
exposure
and
more
than
10­
years
of
exposure.
Reference:
Bhat,
M.
R.
and
Ramaswamy,
C.
1993.
Effect
of
ammonia,
urea
and
diammonium
phosphate
(
DAP)
on
lung
functions
in
fertilizer
plant
workers.
Indian
J.
Physiol.
Pharmacol.
37:
222­
224.
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
35
6.
REFERENCES
21
CFR
582.1141
as
cited
in
Hazardous
Substances
Data
Bank
(
HSDB).

American
Public
Health
Association
(
APHA).
1985.
Standard
Methods
for
the
Examination
of
Water
and
Wastewater.
Washington,
D.
C.

Aufderheide,
J.
A.
and
Bussard,
J.
B.
2000.
Toxicity
of
Diammonium
Phosphate
(
DAP)
to
the
Unicellular
Green
Alga,
Selenastrum
Capricornutum.
ABC
Study
No.
46207.

Bhat,
M.
R.
and
Ramaswamy,
C.
1993.
Effect
of
ammonia,
urea
and
diammonium
phosphate
(
DAP)
on
lung
functions
in
fertilizer
plant
workers.
Indian
J.
Physiol.
Pharmacol.
37:
222­
224.

Budavari,
S.
1996.
The
Merck
Index.
Whitehouse
Station:
Merck
Research
Laboratories.

European
Commission
2000.
Diammonium
Hydrogenorthophosphate.
International
Uniform
Chemical
Information
Database.
Year
2000
CD
 
ROM
edition.

Gudi,
R.
and
Brown,
C.
2001.
In
Vitro
Mammalian
Chromosome
Aberration
Test:
Diammonium
Phosphate
(
DAP).
BioReliance.
Sponsor
Project
Number
TFI002­
CHO.

Hazardous
Substances
Data
Bank
(
HSDB).
1999.
Diammonium
phosphate.
National
Library
of
Medicine,
Bethesda,
MD.

Huntingdon
Life
Sciences
Ltd.
2002.
DAP:
4­
Week
General
Toxicity
and
Reproductive/
Developmental
Toxicity
Screening
Test.
Study
Number
WBG
001/
022948.

Inman,
R.
C.
1974.
Acute
toxicity
of
Phos­
Check
®
202
and
Diammonium
Phosphate
to
Fathead
Minnows.
U.
S.
Air
Force
Environmental
Health
Laboratory,
Kelly
Air
Force
Base,
TX.
Report
EHL(
K)
74­
31.

IPCS.
1993.
International
Chemical
Safety
Cards
No.
0217.
Ammonium
Phosphate
Dibasic.

Johnson,
W.
W.
and
Finley,
M.
T.
1980.
Handbook
of
Acute
Toxicity
of
Chemicals
to
Fish
and
Aquatic
Invertebrates.
USDOI,
Fish
&
Wildlife
Service
Resource
Publication
137.

Johnson,
W.
W.
and
Sanders,
H.
O.
1977.
Chemical
forest
fire
retardants:
Acute
toxicity
to
five
freshwater
fishes
and
a
scud.
Technical
Papers
of
the
U.
S.
Fish
and
Wildlife
Service.

Lewis,
Jr.,
R.
J.
1992.
Sax's
Dangerous
Properties
of
Industrial
Materials.
8th
ed.
Vol.
II.
New
York:
Van
Nostrand
Reinhold.
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
36
Lide,
D.
R.
1992.
CRC
Handbook
of
Chemistry
and
Physics.
Boston:
CRC
Press.

Merkel,
D.
J.
2000.
Acute
dermal
toxicity
study
in
rats­
limit
test.
Product
Safety
Labs
study
number
9736.

Merkel,
D.
J.
2000.
Acute
oral
toxicity:
Up
and
down
procedure
in
rats­
limit
test.
Product
Safety
Labs
study
number
9733.

Naqvi,
T.
S.,
Naqvi.
M.
S.,
and
Singh,
R.
K.
1993.
Effect
of
fertilizer
diammonium
phosphate
on
liver,
kidney
and
muscle
5'­
nucleotidase
activity
of
fresh
water
teleost
fish
Clarias
batrachus.
Biomedical
and
Environmental
Sciences
6:
385­
388.

Palanichamy,
S.,
Arunachalam,
S.,
and
Balasubramanian,
M.
P.
1985.
Food
consumption
of
Sarotherodon
mossambicus
(
Trewaves)
exposed
to
sublethal
concentration
of
diammonium
phosphate.
Hydrobiologia
128:
233­
237.

Sarkar,
S.
K.
1997.
Toxicity
of
simazine,
diammonium
phosphate
and
rock
phosphate
to
fish,
snail
and
worm
at
various
water
temperatures.
Geobios
24:
142­
145.

Singh,
R.,
Singh,
R.
K.,
and
Pande,
P.
N.
1995.
Biochemical
toxicity
of
fertilizer
diammonium
phosphate
to
fish
Clarias
batrachus.
Uttar
Pradesh
J.
Zool.
15(
2):
144­
148.

Trivedi,
S.
P.,
Sethi,
N.,
Singh,
S.
K.
and
Singh,
P.
1989.
Impact
of
fertilizer,
diammonium
phosphate,
on
haematological
profile
of
teleost
fish,
Heteropneutes
fossilis.
Bio.
Mem.
15:
166­
174.

Trivedi,
S.
P.,
Singh,
P.,
Sethi,
N.
and
Singh,
R.
K.
1990.
Evaluations
of
hematotoxic
effects
of
two
commonly
used
fertilizers,
diammonium
phosphate
and
urea,
on
fish
Clarias
batrachus.
Ecotoxicology
and
Environmental
Safety.
19:
135­
142.

United
Nations
Industrial
Development
Organization
(
UNIDO)
and
International
Fertilizer
Development
Center
(
IFDC).
1998.
Fertilizer
Manual.
Kluwer
Academic
Publishers,
The
Netherlands.

USEPA.
2000.
EPISUITE
Estimation
Program
V.
3.10.
US
Environmental
Protection
Agency.

Wagner,
V.
O.
and
Klug,
M.
L.
2001.
Bacterial
Reverse
Mutation
Assay:
Diammonium
Phosphate
(
DAP).
BioReliance.
Sponsor
Project
Number
TFI002­
AMES.
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
APPENDIX
A
 
SIDS
Data
Availability
Summary
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
Explanation
of
Appendix
A
Appendix
A
is
a
SIDS
Data
Summary
table
that
provides
an
overview
of
availability
and
quality
of
the
data
used
in
this
Health
and
Environmental
Safety
Data
Summary
for
Diammonium
Phosphate
(
DAP).
Y
(
Yes)
and
N
(
No)
designations
indicate
whether
data
are
available
that
meet
the
criteria
for
each
column.
The
columns
are
described
as:

Information:
Data
exist
that
were
useful
for
describing
the
data
element.
These
data
may
be
from
standard
laboratory
tests
or
from
generally
recognized
published
sources
or
professional
experience.

OECD
Study:
The
data
were
developed
using
standard
OECD
or
essentially
similar
(
e.
g.,
EPA
harmonized
protocols)
guidelines.

GLP:
The
data
were
developed
under
standard
Good
Laboratory
Practice
provisions.
These
generally
represent
the
highest
quality
data.

Other
Study:
Studies
were
conducted
that
could
not
be
definitively
identified
as
following
OECD
or
GLP
protocols.
These
studies
were
included
when
they
were
determined
to
be
of
adequate
quality
and
provided
relevant
information
to
the
characterization
of
the
compound.

Estimation
Method:
In
some
cases,
data
may
be
estimated
using
established
structure
activity
relationships.
These
methods
are
common
for
physical­
chemical
parameters
such
as
octanolwater
partition
coefficients
and
water
solubility.

Acceptable:
This
column
indicates
whether
the
data
are
deemed
acceptable
by
standard
acceptability
criteria
and
professional
judgment.
Only
the
data
meeting
adequacy
standards
are
included
in
this
summary
document.

SIDS
Testing
Recommended:
This
column
indicates
whether
additional
testing
is
recommended
based
on
an
evaluation
of
the
available
data
summarized
in
this
document.
It
is
generally
not
necessary
to
fill
all
of
the
apparent
data
gaps
in
order
to
adequately
characterize
the
inherent
hazard
of
chemicals.
Information
from
other
data
elements
and
from
other
chemicals
in
the
category,
along
with
professional
judgment,
are
useful
in
the
final
determination
of
the
need
for
further
testing.
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
SIDS
DATA
AVAILABILITY
SUMMARY
DATE:
January
27,
2003
Diammonium
Phosphate
(
DAP)
CAS
No:
1336­
21­
6
Information
OECD
Study
GLP
Other
Study
Estimated
Method
Acceptable
SIDS
Testing
Recommended
STUDY
Y/
N
Y/
N
Y/
N
Y/
N
Y/
N
Y/
N
Y/
N
PHYSICAL­
CHEMICAL
DATA
2.1
2.2
2.3
2.4
2.5
2.6
2.8
2.9
2.10
2.11
2.12
Melting
Point
Boiling
Point
Density
Vapor
Pressure
Partition
Coefficient
Water
Solubility
pH
and
pKa
Values
Autoflammability
Flammability
Explosive
Properties
Oxidizing
Properties
Oxidation:
Reduction
Potential
Y
Y
Y
Y
N
Y
Y
Y
Y
Y
Y
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
Y
Y
Y
Y
­
Y
Y
Y
Y
Y
Y
­
N
N
N
N
N
N
N
N
N
N
N
N
OTHER
P/
C
STUDIES
RECEIVED
Y
N
N
N
N
Y
N
ENVIRONMENTAL
FATE
and
PATHWAY
3.1.1
3.1.2
3.1.3
3.2
3.3
3.5
3.7
Photodegradation
Stability
in
Water
Stability
in
Soil
Monitoring
Data
Transport
and
Distribution
Biodegradation
Bioaccumulation
N
Y
Y
N
Y
Y
Y
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
Y
N
­
Y
Y
­
Y
Y
Y
N
N
N
N
N
N
N
OTHER
ENV
FATE
STUDIES
RECEIVED
N
N
N
N
N
­
N
ECOTOXICITY
4.1
4.2A
4.2B
4.3
4.5.1
4.5.2
4.6.1
4.6.2
4.6.3
Acute
Toxicity
to
Fish
Acute
Toxicity
to
Daphnia
Acute
Toxicity
to
Other
Aquatic
Organisms
Toxicity
to
Algae
Chronic
Toxicity
to
Fish
Chronic
Toxicity
to
Daphnia
Toxicity
to
Soil
Dwelling
Organisms
Toxicity
to
Terrestrial
Plants
Toxicity
to
Birds
Y
N
Y
Y
N
N
N
N
N
N
N
N
Y
N
N
N
N
N
N
N
N
Y
N
N
N
N
N
Y
N
Y
N
N
N
N
N
N
N
N
N
N
N
N
N
N
N
Y
­
Y
Y
­
­
­
­
­
N
N
N
N
N
N
N
N
N
OTHER
ECOTOXICITY
STUDIES
RECEIVED
Y
N
N
Y
N
Y
N
TOXICITY
5.1.1
5.1.2
5.1.3
5.2
5.4
5.5
5.6
5.8
5.9
5.11
Acute
Oral
Acute
Inhalation
Acute
Dermal
Skin/
Eye
Irritation
Repeated
Dose
Genetic
Toxicity
in
vitro
.
Gene
mutation
.
Chromosomal
aberration
Genetic
Toxicity
in
vivo
Reproduction
Toxicity
Development/
Teratogenicity
Human
Experience
Y
N
Y
N
Y
Y
Y
N
Y
Y
Y
Y
N
Y
N
Y
Y
Y
N
Y
Y
N
Y
N
Y
N
Y
Y
Y
N
Y
Y
N
N
N
N
N
N
N
N
N
N
N
Y
N
N
N
N
N
N
N
N
N
N
N
Y
­
Y
­
­

Y
Y
­
Y
Y
Y
N
N
N
N
N
N
N
N
N
N
N
OTHER
TOXICITY
STUDIES
RECEIVED
Y
N
N
Y
N
Y
N
Diammonium
Phosphate
(
DAP)
CAS
#
7783­
28­
0
Appendix
B
 
SIDS
Data
Summaries
for
the
Phosphate
Compounds
Category:
Diammonium
Phosphate,
Liquid
Polyphosphate,
Monoammonium
Phosphate,
and
Super
Phosphates
SIDS
DATA
SUMMARIES
FOR
THE
PHOSPHATE
CATEGORY
Date:
January
27,
2003
Diammonium
Phosphate
(
DAP)

CAS
#
7783­
28­
0
Diammonium
Phosphate
Liquid
Polyphosphate
Monoammonium
Phosphate
DATA
ELEMENTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
PHYSICAL­
CHEMICAL
DATA
2.1
Melting
Point
155
°
C
190
°
C
2.2
Boiling
Point
Decomposes
Decomposes
2.3
Density
0.93­
1.619
g/
mL
at
20oC
1.43
g/
mL
at
60oC
1.83
g/
mL
at
25oC
2.4
Vapor
Pressure
<
100
Pa
at
20
°
C
<
100
Pa
at
20
°
C
2.5
Octanol/
Water
Partition
Coefficient
2.6A
Water
Solubility
588
g/
L
at
20
°
C
Miscible
328
g/
L
at
20
°
C
2.6B
pH
and
pKa
values
~
8
6.5­
7
4.2
in
0.2M
aqueous
solution
2.7
Flash
Point
Not
applicable
Not
flammable
Not
applicable
2.8
Auto
Flammability
Not
flammable
Not
applicable
Not
flammable
2.9
Flammability
Not
flammable
Not
flammable
Not
flammable
2.10
Explosive
Properties
Not
explosive
Not
explosive
Not
explosive
2.11
Oxidizing
Properties
Not
an
oxidizer
Not
an
oxidizer
2.12
Oxidation:

Reduction
Potential
2.13A
Additional
Data
See
text
SIDS
DATA
SUMMARIES
FOR
THE
PHOSPHATE
CATEGORY
Date:
January
27,
2003
Diammonium
Phosphate
(
DAP)

CAS
#
7783­
28­
0
Diammonium
Phosphate
Liquid
Polyphosphate
Monoammonium
Phosphate
DATA
ELEMENTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
ENVIRONMENTAL
FATE
and
PATHWAY
3.1.1
Photodegradation
3.1.2
Stability
in
Water
Stable
Stable
Stable
3.1.3
Stability
in
Soil
Stable
Behave
as
salts
Stable
3.3.2
Distribution
Calculated,

Fugacity
Level
III
6.5
x
10­
15%
to
air
45.3%
to
water
54.6%
to
soil
0.0755%
to
sediment
Calculated,

Fugacity
Level
III
3.98
x
10­
12%
to
air
45.3%
to
water
54.6%
to
soil
0.0755%
to
sediment
3.5
Biodegradation
Phosphorus
cycles
is
well
understood
Phosphorus
cycles
is
well
understood
Phosphorus
cycles
is
well
understood
3.7
Bioaccumulation
Does
not
bioaccumulate
Does
not
bioaccumulate
Does
not
bioaccumulate
SIDS
DATA
SUMMARIES
FOR
THE
PHOSPHATE
CATEGORY
Date:
January
27,
2003
Diammonium
Phosphate
(
DAP)

CAS
#
7783­
28­
0
Diammonium
Phosphate
Liquid
Polyphosphate
Monoammonium
Phosphate
DATA
ELEMENTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
ECOTOXICITY
4.1
Acute
toxicity
to
Fish
Coho
salmon,
Chinook
salmon,
Rainbow
trout,
Bluegill,
Largemout
h
bass,
Tilapia,
Fathead
minnow
96
hrs
LC50
=
90­
1,875
mg/
L
Oncorhync
hus
mykiss
96
hrs
LC50
=
>
101
mg/
L
Oncorhync
hus
mykiss
96
hrs
LC50
=
>
85.9
mg/
L
4.2
Acute
Toxicity
to
Aquatic
Invertebrates
Daphnia
magna
Amphipod
Snail,
Worm
96
hrs
96
hrs
LC50
=
40­
52
mg/
L
LC50
=
1,005­

2,472
mg/
L
4.3
Toxicity
to
Aquatic
Plants
(
Algae)
Selenastrum
capricornutum
72
hrs
NOEC
(
stimulation)
=

3.57
mg
DAP/
L
NOEC
(
toxicity)

=
97.1
mg
DAP/
L
4.4
Toxicity
to
Bacteria
4.5.1
Chronic
Toxicity
to
Fish
4.5.2
Chronic
Toxicity
to
Aquatic
Invertebrates
4.6.1
Toxicity
to
Soil
Dwelling
Organisms
SIDS
DATA
SUMMARIES
FOR
THE
PHOSPHATE
CATEGORY
Date:
January
27,
2003
Diammonium
Phosphate
(
DAP)

CAS
#
7783­
28­
0
Diammonium
Phosphate
Liquid
Polyphosphate
Monoammonium
Phosphate
DATA
ELEMENTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
4.6.2
Toxicity
to
Terrestrial
Plants
4.6.3
Toxicity
to
Other
Non­
Mammalian
Terrestrial
Species
SIDS
DATA
SUMMARIES
FOR
THE
PHOSPHATE
CATEGORY
Date:
January
27,
2003
Diammonium
Phosphate
(
DAP)

CAS
#
7783­
28­
0
Diammonium
Phosphate
Liquid
Polyphosphate
Monoammonium
Phosphate
DATA
ELEMENTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
TOXICITY
5.1.1
Acute
Oral
Toxicity
Rat
OECD
425
LD50
>
2,000
mg/
kg
bw
Rat
OECD
425
LD50
>
2,000
mg/
kg
bw
Rat
OECD
425
LD50
>
2000
mg/
kg
bw
5.1.2
Acute
Inhalation
5.1.3
Acute
Dermal
Toxicity
Rat
OECD
402
LD50
>
5000
mg/
kg
bw
Rat
OECD
402
LD50
>
5,000
mg/
kg
bw
Rat
OECD
402
LD50
>
5000
mg/
kg
bw
5.1.4
Acute
Toxicity,

Other
Routes
5.2.1
Skin
Irritation/

Corrosion
Irritant
Mild
irritant
5.2.2
Eye
Irritation/

Corrosion
Irritant
Mild
irritant
5.3
Skin
Sensitization
5.4
Repeated
Dose
Rat
OECD
422
NOAEL
=
250
mg/
kg/
day
5.5
Genetic
Toxicity
in
vitro
.
Gene
mutation
Salmonella
typhimurium
Bacterial
reverse
mutation
assay
(
OECD
471)
Negative
.
Chromosomal
aberration
Chinese
hamster
ovaries
Chromosome
aberration
test
(
OECD
473)
Negative
5.6
Genetic
Toxicity
in
vivo
5.7
Carcinogenicity
5.8
Toxicity
to
Reproduction
Rat
OECD
422
NOAEL
=
1500
mg/
kg/
day
5.9
Development
Toxicity/
Teratogenicity
Rat
OECD
422
NOAEL
=
1500
mg/
kg/
day
5.10
Metabolism
and
Toxicokinetics
See
text
See
text
5.11
Human
Experience
See
text
See
text
See
text
SIDS
DATA
SUMMARIES
FOR
THE
PHOSPHATE
CATEGORY
Date:
January
27,
2003
Diammonium
Phosphate
(
DAP)

CAS
#
7783­
28­
0
Diammonium
Phosphate
Liquid
Polyphosphate
Monoammonium
Phosphate
DATA
ELEMENTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
1.8
Occupational
Exposure
Limits
TLV
as
inert
dust
15
mg/
m3
No
TLV
established
TLV
as
inert
dust
15
mg/
m3
SIDS
DATA
SUMMARIES
FOR
THE
PHOSPHATE
CATEGORY
Date:
January
27,
2003
Diammonium
Phosphate
(
DAP)

CAS
#
7783­
28­
0
Super
Phosphates
DATA
ELEMENTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
PHYSICAL­
CHEMICAL
DATA
2.1
Melting
Point
110
°
C
2.2
Boiling
Point
2.3
Density
1,035
kg/
m3
2.4
Vapor
Pressure
2.5
Octanol/
Water
Partition
Coefficient
2.6A
Water
Solubility
18
g/
L
at
20
°
C
2.6B
pH
and
pKa
values
2.8­
2.9
in
10%

water
suspension
2.7
Flash
Point
Not
applicable
2.8
Auto
Flammability
2.9
Flammability
2.10
Explosive
Properties
2.11
Oxidizing
Properties
2.12
Oxidation:

Reduction
Potential
2.13A
Additional
Data
SIDS
DATA
SUMMARIES
FOR
THE
PHOSPHATE
CATEGORY
Date:
January
27,
2003
Diammonium
Phosphate
(
DAP)

CAS
#
7783­
28­
0
Super
Phosphates
DATA
ELEMENTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
ENVIRONMENTAL
FATE
and
PATHWAY
3.1.1
Photodegradation
3.1.2
Stability
in
Water
3.1.3
Stability
in
Soil
Stable
3.3.2
Distribution
Calculated,

Fugacity
Level
III
1x10­
6%
to
air
49.8%
to
water
50.1%
to
soil
0.092%
to
sediment
3.5
Biodegradation
Biodegradable
3.7
Bioaccumulation
SIDS
DATA
SUMMARIES
FOR
THE
PHOSPHATE
CATEGORY
Date:
January
27,
2003
Diammonium
Phosphate
(
DAP)

CAS
#
7783­
28­
0
Super
Phosphates
DATA
ELEMENTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
ECOTOXICITY
4.1
Acute
toxicity
to
Fish
Labeo
rohita,
Catla
catla,
Cirrhinus
mrigala,
Cyprinus
carpio,
Tilapia
mossambica
96
hrs
LC50
=
1,560­

5,900
ppm
4.2A
Acute
Toxicity
to
Aquatic
Invertebrates
Daphnia
carinata
LC50
=
1,790­

1,825
mg/
L
4.2B
Acute
Toxicity
to
Other
Aquatic
Organisms
Moina
micrura,

Cyclops
viridis
72
hrs
LC50
=
1,625­

2,305
ppm
Branchiura
sowerbyi,
Planorbis
exustus,
Lymnaea
leuteola,
Viviparus
bengalensis,

Chironomus
sp
96
hrs
LC50
=
1,133­

5,005
ppm
4.3
Toxicity
to
Aquatic
Plants
(
Algae)
Selenastrum
capricornutum
OECD
201
NOEC
(
toxicity)
=

87.6
mg/
L
NOEC
(
stimulation)
=

21.6
mg/
L
4.4
Toxicity
to
Bacteria
SIDS
DATA
SUMMARIES
FOR
THE
PHOSPHATE
CATEGORY
Date:
January
27,
2003
Diammonium
Phosphate
(
DAP)

CAS
#
7783­
28­
0
Super
Phosphates
DATA
ELEMENTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
4.5.1
Chronic
Toxicity
to
Fish
4.5.2
Chronic
Toxicity
to
Aquatic
Invertebrates
4.6.1
Toxicity
to
Soil
Dwelling
Aquatic
Invertebrates
4.6.2
Toxicity
to
Terrestrial
Plants
See
text
4.6.3
Toxicity
to
Other
Non­
Mammalian
Terrestrial
Species
SIDS
DATA
SUMMARIES
FOR
THE
PHOSPHATE
CATEGORY
Date:
January
27,
2003
Diammonium
Phosphate
(
DAP)

CAS
#
7783­
28­
0
Super
Phosphates
DATA
ELEMENTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
TOXICITY
5.1.1
Acute
Oral
Toxicity
Sheep
LD50
=
5,000­

6,000
mg/
kg
bw
5.1.2
Acute
Inhalation
5.1.3
Acute
Dermal
Toxicity
5.1.4
Acute
Toxicity,

Other
Routes
5.2.1
Skin
Irritation/

Corrosion
Mild
irritant
5.2.2
Eye
Irritation/

Corrosion
Mild
irritant
5.3
Skin
Sensitization
5.4
Repeated
Dose
Rat
OECD
422
NOAEL
=
750
mg/
kg/
day
5.5
Genetic
Toxicity
in
vitro
.
Gene
mutation
Salmonella
typhimurium
OECD
471
Negative
.
Chromosomal
aberration
Chinese
hamster
ovaries
OECD
473
Negative
5.6
Genetic
Toxicity
in
vivo
Mice
Chromosome
aberration
Clastogenic,

possibly
mutagenic
5.7
Carcinogenicity
5.8
Toxicity
to
Reproduction
Rat
OECD
422
NOAEL
=
750
mg/
kg/
day
5.9
Development
Toxicity/
Teratogenicity
Rat
OECD
422
NOAEL
=
750
mg/
kg/
day
SIDS
DATA
SUMMARIES
FOR
THE
PHOSPHATE
CATEGORY
Date:
January
27,
2003
Diammonium
Phosphate
(
DAP)

CAS
#
7783­
28­
0
Super
Phosphates
DATA
ELEMENTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
5.10
Metabolism
and
Toxicokinetics
See
text
5.11
Human
Experience
See
text
1.8
Occupational
Exposure
Limits
TLV
as
inert
dust
15
mg/
m3