Document ID: EPA-HQ-OPPT-2014-0766-0011
Agency: epa
Document Type: Supporting & Related Material
Title: 
Posted Date: 2015-02-25T05:00Z

Note to Docket ID., EPA-HQ-OPPT-2014-0766-0002, OCSPP 890.2100: Avian Two-generation Toxicity Test in the Japanese Quail (JQTT), Public Draft dated 12/172014.  Errata  -  References to Appendix 1 and 7.
1. Reference to Appendix 1, p. 13-14 is incorrect [see underlined text].  The correct reference is Appendix 6, Histopathology Guidance.
a. First reference, p. 12, Middle of the page.
"...Organs including kidneys, liver, spleen, adrenal glands, reproductive organs and associated structures, cloacal gland, bursa, and right thyroid gland should be excised and preserved in Davidson's fixative or a suitable alternative for histopathological examination.  A detailed description of excision, fixation, and tissue preparation is provided in Appendix I (pages 3-41) of this document." Correct reference is Appendix 6 (no page numbers).
b. Second reference, p. 13, Top of the page.
"...14 day old chicks will be fixed in situ.  A detailed description of excision, fixation, and tissue preparation is provided in Appendix I (pages 93- 114) of this document."   Correct reference is Appendix 6 (no page numbers).
2.  p. 14, Incorrect Reference to Appendix 7.  The correct reference should be Appendix 5, Recommendations for Preparation of Samples, where there is a detailed description of sample preparation for blood, thyroid gland, and yolk.

a. Incorrect reference to Appendix 7 (p. 14) appears in the following two sentences:

A detailed description of blood, thyroid gland, and yolk preparation for thyroid hormone and immunoassay analyses are found in Appendix 7."

"A detailed description of blood, thyroid gland, and yolk preparation for steroid hormone and immunoassay analyses are found in Appendix 7."

b. Correct reference to Appendix 5. The sentence (it appears twice) should be rewritten as follows, "A detailed description of blood, thyroid gland, and yolk preparation for thyroid (or steroid) hormone analysis and immunoassay is found in Appendix 5."