Document ID: EPA-HQ-OPP-2003-0024-0022
Agency: epa
Document Type: Supporting & Related Material
Title: 
Posted Date: 2003-06-04T04:00Z

Data
Evaluation
Report
on
South
African
Analytical
Support
 
Hormone
and
Aromatase
Analysis
EPA
MRID
Number
458675­
01
Data
Requirement:
:
EPA
DP
Barcode
D288775
EPA
MRID
458675­
01
EPA
Guideline
70­
1(
Special
Study)

Test
material:
Purity:
not
reported
Common
name
Atrazine
Chemical
name:
IUPAC
CAS
name
6­
chloro­
N­
ethyl­
N'­(
1­
methylethyl)­
1,3,5­
triazine­
2,4­
diamine
CAS
No.
1912­
24­
9
Synonyms
EPA
PC
Code:
80803
Primary
Reviewer:
Thomas
M.
Steeger,
Ph.
D.,
Senior
Biologist
Date:
April
1,
2003
Environmental
Fate
and
Effects
Division,
ERB
4,
U.
S.
Environmental
Protection
Agency
Secondary
Reviewer(
s):
Joseph
E.
Tietge,
M.
S.,
Research
Aquatic
Biologist
Date:
Mid­
Continent
Ecology
Division,
National
Health
and
Environmental
Effects
Research
Laboratory
(
Duluth),
U.
S.
Environmental
Protection
Agency
Stephanie
Irene,
Ph.
D.,
Senior
Advisor
Date:
Environmental
Fate
and
Effects
Division,
ERB
3,
U.
S.
Environmental
Protection
Agency
Mary
J.
Frankenberry,
Senior
Statistician
Date:
Environmental
Fate
and
Effects
Division,
ERB
3,
U.
S.
Environmental
Protection
Agency
EPA
PC
Code
080803
Date
Evaluation
Completed:
06/
01/
2003
CITATION:
Giesy,
J.
P.,
M.
Hecker,
and
P.
D.
Jones.
2003.
South
African
Analytical
Support
 
Hormone
and
Aromatase
Analysis
(
SA­
01C).
Aquatic
Toxicology
Laboratory,
Michigan
State
University,
National
Food
Safety
and
Toxicology
Center,
E.
Lansing,
MI.
Sponsor:
Syngenta
Crop
Protection,
Inc.,
Laboratory
Study
ID
ECORISK
Number
MSU­
07.
Data
Evaluation
Report
on
South
African
Analytical
Support
 
Hormone
and
Aromatase
Analysis
EPA
MRID
Number
458675­
01
Page
2
of
152
Data
Evaluation
Report
on
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Support
 
Hormone
and
Aromatase
Analysis
EPA
MRID
Number
458675­
01
Page
3
of
152
EXECUTIVE
SUMMARY:

A
field
study
was
conducted
in
corn­
growing
(
experimental)
and
non­
corn
growing
(
reference)
areas
in
the
Potchefstroom
region
of
South
Africa
to
determine
whether
exposure
to
atrazine
and
related
triazines
could
affect
aromatization
of
testosterone
to
estradiol
in
wild
populations
of
African
clawed
frogs
(
Xenopus
laevis)
In
this
region,
atrazine
is
typically
applied
in
October
to
November.
Most
frogs
were
collected
in
April
and
May
after
the
rainy
season;
however,
in
some
atrazine­
use
areas,
frog
collections
extended
until
mid­
September
due
to
the
low
sample
sizes.
Frogs
were
collected
from
baited
traps
after
48
hours,
transferred
to
the
lab,
and
housed
individually
in
2­
L
plastic
cages
for
48
hours
before
collecting
samples.
Blood
samples
were
collected
for
plasma
testosterone
and
estradiol
analysis
and
gonad
(
one)
samples
were
collected
for
aromatase
activity
analysis.
Snout­
vent
length,
body
weight,
and
gonad
weight
were
measured.
Gonadosomatic
index
(
GSI
=
gonad
weight
÷
body
weight),
condition
index
(
CI
=
body
weight
÷
snout­
vent
length)
and
the
ratio
of
plasma
testosterone
to
estradiol
were
calculated.

Mean
and
median
GSI
were
greater
for
both
males
and
females
in
high
atrazine
exposure
(
experimental)
sites
compared
to
low
atrazine
exposure
(
reference)
sites;
however,
there
was
no
difference
in
body
weight
(
CI)
between
high
and
low
atrazine
exposure
sites.
When
comparisons
were
made
between
ponds
with
the
highest
atrazine
concentrations
to
those
with
the
lowest
atrazine
concentrations,
males
from
high
atrazine
exposure
sites
had
significantly
lower
median
plasma
testosterone
than
males
from
low
atrazine
exposure
sites.
Although
male
plasma
testosterone
was
not
significantly
correlated
with
atrazine
concentrations,
there
was
a
significant
negative
correlation
between
the
log
of
male
plasma
testosterone
levels
and
log
of
the
atrazine
degradate
diaminochlorotriazine
(
DACT)
concentration.
Additionally,
female
plasma
testosterone
levels
were
negatively
correlated
with
atrazine
concentrations,
and
females
collected
from
reference
ponds
had
significantly
higher
testosterone
levels
than
females
collected
from
experimental
sites.

Mean
plasma
estradiol
concentrations
were
significantly
lower
in
experimental
site
males
and
females
compared
to
reference
site
animals.
Male
plasma
estradiol
was
negatively
correlated
with
log
DACT
concentrations,
while
female
estradiol
concentrations
were
negatively
correlated
with
atrazine
concentrations.

Ideally,
a
study
should
be
designed
based
on
the
variability
associated
with
the
measurement
endpoints.
Sample
sizes
should
reflect
the
number
of
test
animals
required
to
identify
a
specified
difference
within
a
given
level
of
certainty.
Potential
sources
of
variability
should
also
be
identified
and
controlled
to
the
extent
possible.
The
current
study
did
not
appear
to
base
its
design
on
the
variability
associated
with
the
range
of
measurement
endpoints.
Animals
were
collected
over
some
fairly
broad
periods
of
time
(
up
to
6
months)
that
would
almost
assure
that
animals
within
the
same
group
would
vary
considerably
in
terms
of
development.
Based
on
the
number
of
animals
collected,
frogs
appeared
to
be
more
abundant
in
reference
sites
than
in
corn­
growing
regions
where
half
as
many
frogs
were
collected.
The
entire
design
was
questionable,
including
extended
sampling
periods
in
ponds
of
questionable
similarity,
baiting
with
potentially
hormone­
laden
liver/
meat,
and
confinement
in
cages
at
unknown
loading
rates.
These
factors
could
confound
the
study's
ability
to
reliably
and
reasonably
quantify
what
"
typical"
steroid
hormone
levels
and/
or
aromatase
activity
is
for
Xenopus.
Additionally,
the
presence
of
atrazine
and
triazine
degradates
across
all
sites
(
reference
and
corn­
growing)
and
the
failure
of
the
study
to
characterize
the
suite
of
pesticides
other
than
triazines
in
the
study
sites
contributes
to
the
uncertain
utility
of
this
study
in
attempting
to
document
potential
effects
of
atrazine.

In
order
for
such
a
study
to
be
of
value,
the
authors
would
need
to
conduct
a
pilot
study
first
to
establish
the
necessary
sample
size
required
to
detect
a
specified
difference
in
the
biological
attributes
being
measured
within
a
certain
level
of
confidence.
The
sample
size
would
be
based
on
the
variability
associated
with
a
particular
Data
Evaluation
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parameter.
Unfortunately,
the
levels
of
variability
associated
with
the
parameters
of
interest
in
this
study
would
likely
require
very
high
sample
sizes.
A
more
practical
approach
would
be
to
identify
major
sources
of
variability
and
attempt
to
better
control
those
conditions.

The
current
data
suggest
that
atrazine
and/
or
its
degradates
may
be
impacting
plasma
testosterone;
however,
the
data
are
not
conclusive
and
the
mechanism
underlying
this
phenomena
can
not
be
identified
based
on
this
study.

I.
MATERIALS
AND
METHODS
GUIDELINE
FOLLOWED:
Nonguideline
Study
COMPLIANCE:
Not
conducted
under
full
GLP;
however,
most
practices
as
defined
by
40
CFR
Part
160,
August
19,
1989
were
established
for
this
study,
including
but
not
limited
to:

°
Written,
authorized
protocol
°
Written,
authorized
Standard
Operating
Procedures
for
all
key
procedures.

°
Organization
and
Personnel
were
sufficient
in
terms
of
number,
education,
training
and
experience.

°
Facilities
were
of
suitable
size
and
construction
°
Equipment
used
was
of
appropriate
design
and
adequate
capacity.

°
Independent
QA
Inspections
were
conducted.

°
Phase
Report
was
written
°
Raw
data,
documentation,
records,
protocols,
and
final
phase
report
will
be
archieved.

A.
MATERIALS:

1.
Test
Material
Atrazine
Description:
Not
reported
Lot
No./
Batch
No.
:
Not
reported
Purity:
Not
reported
Data
Evaluation
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Stability
of
compound
under
test
conditions:
Not
reported
Storage
conditions
of
test
chemicals:
Not
reported
2.
Test
organism:

Species:
African
clawed
frog
(
Xenopus
laevis)

.

Age
at
test
initiation:
Adults
Weight
at
study
initiation
(
mean
and
range):
Not
reported
Length
at
study
initiation
(
mean
and
range):
Not
reported
Source:
Adult
X.
laevis
were
field
collected
in
two
areas
(
non­
corn
growing
and
corn
growing
)
in
the
vicinity
of
Potchefstrooom,
South
Africa,
using
traps
baited
with
liver
and
meat
scraps.
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B.
STUDY
DESIGN:

Objective:
1.
To
determine
whether
exposure
to
atrazine
and
related
triazines
under
field
conditions
in
a
major
corn­
growing
area
in
South
Africa
could
affect
aromatization
of
testosterone
and
estradiol
in
wild
populations
of
clawed
frogs
(
X.
laevis)
and
more
specifically:

a.
to
determine
if
exposure
to
triazines
used
in
corn
production
affects
plasma
concentrations
of
the
sex
steroid
hormones
T
and
E2
in
male
and
female
X.
laevis
adults;

b.
to
determine
whether
there
are
alterations
in
aromatase
activity
in
adult
X.
laevis
in
both
sexes
in
response
to
exposure
to
triazines;

c.
to
evaluate
the
range
of
triazine
concentrations,
if
any,
that
might
lead
to
changes
in
plasma
hormone
concentrations
and
aromatase
activity
synthesis;
and
d.
to
evaluate
the
possible
relevance
of
triazine­
mediated
alterations
in
the
aromatization
of
T
to
E2
to
reproduction.

1.
Experimental
Conditions
a)
Range­
finding
Study:

b)
Definitive
Study
Table
1
.
Experimental
Parameters
Sampling
was
conducted
in
April
and
May
approximately
6
months
after
atrazine
was
applied
(
October
/

November);
water
samples
to
characterize
exposure
was
collected
one
month
prior
to
frog
sampling.

Environmental
parameters
measured
at
each
site
included
habitat
description
(
vegetative
cover
and
water
depth).
Water
samples
were
analyzed
for
atrazine,
related
triazines,
other
pesticides
and
metals.
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A
total
of
eight
sites
in
two
adjacent
regions
(
Viljoenskroon
corn
growing
region
=
E
for
experimental
were
sampled;
Potchefstroom
non­
corn
growing
region
=
C
or
R
or
control/
reference)
in
South
Africa.

Compounds
of
interest
included
atrazine,
its
metabolites
desethylated
atrazine
(
DEA),
desisopropyl
atrazine
(
DIA),
diaminochlorotriazine
(
DACT),
plus
terbutylazine,
simazine
and
acetochlor.

Non­
corn
growing
sites
had
secchi
disc
readings
ranging
from
6.5
to
32
cm;
pH
ranged
from
5.1
to
8.8;
some
of
the
control
ponds
were
subject
to
drying
(
semi­
permanent).

Corn­
growing
sites
had
surface
areas
ranging
from
2,400
m2
to
68,000
m2;
pH
ranged
from
7.2
­
10.8
and
upper
secchi
disc
value
of
207
cm.

Frogs
were
sampled
after
the
rainy
season
in
April
and
May;
however,
at
atrazine
site
E8,
frogs
were
sampled
over
a
six
­
month
period
(
April
­
September)
due
to
low
sample
sizes.
Sampling
at
all
other
sites
took
place
once
or
twice
within
a
"
few
days."
Frogs
were
collected
in
10
baited
traps
and
harvested
after
two
days.

Afterwards,
animals
were
housed
individually
in
2­
L
plastic
containers
for
48
hours
to
recover
from
capture
stress.
Blood
was
collected
by
cardiac
puncture;
gonads
were
removed,
weighed
and
measured.
One
gonad
was
fixed
in
10%
neutral
buffered
formalin
or
Bouin's,
while
the
second
was
snap
frozen.
Gonadosomatic
index
(
weight
of
the
gonad
÷
snout­
vent
length)
and
the
condition
index
(
body
weight
÷
snout­
vent
length)
were
calculated.

Concentrations
of
testosterone
(
T)
and
estradiol
(
E2)
in
blood
plasma
were
measured
by
competitive
ELISA
as
described
by
Cuisset
et
al.
1994
with
modifications
by
Hecker
et
al.
2002
using
COSTAR
high
binding
plates.
Working
ranges
of
the
assays
were
from
0.78
to
800
pg/
well
for
both
testosterone
and
estradiol.

Aromatase
activity
was
measured
following
the
protocol
from
Lephart
and
Simpson
1991
with
minor
modifications
(
Sanderson
2000).
Less
than
0.5
g
of
gonad
was
homogenized.
Data
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II.
RESULTS
and
DISCUSSION:
[
All
results
discussed
in
this
section
and
the
next
are
those
reported
by
the
study
authors.
Although
supplemental
data
are
typically
used
in
a
qualitative
manner
only,
EFED
verified
spreadsheet
data
and
ran
basic
statistical
analyses
on
the
major
study
parameters.
See
attached
appendix.
If
results
appeared
to
differ
in
any
substantive
way,
the
difference
was
reported
in
the
text
below.]

Atrazine
Concentrations
With
the
exception
of
site
E8
(
peak
concentration
in
late
January
at
9

g/
l),
the
greatest
concentrations
of
atrazine
in
pond
water
were
observed
at
the
end
of
the
rainy
season
(
March
through
May);
highest
atrazine
concentrations
were
measured
at
sites
E1
(
4.1

g/
L),
E6
(
3.9

g/
L)
and
E8
(
3.5

g/
L).
Concentrations
at
C1,

C3,
C6
and
E4
were
low
but
could
be
quantified.
The
log
concentrations
of
triazines
DIA,
DEA
and
atrazine
were
significantly
correlated
with
pH
of
the
water.
(
p
<
0.034,
r­
range:
0.770
­
0.802).
Log
DIA
also
showed
a
positive
linear
regression
with
log
temperature
(
7
=
0.801;
p=
0.017)
and
log
visual
depth
®
=
0.814;
p
=

0.014).
Monitoring
data
(
Table
1)
indicated
that
measurable
levels
of
atrazine
were
detected
at
all
sampling
sites
(
range
0.2
­
3.53

g/
L).
The
atrazine
degradate
desisopropyl
atrazine
(
DIA)
was
as
high
in
C3
(
0.61

g/
L)
as
in
many
of
the
corn­
growing
sites,
while
diaminochlorotriazine
(
DACT)
in
C1
(
3.91

g/
L)
was
the
second
highest
concentration
of
the
degradate
detected
at
any
of
the
sampling
sites.
Mean­
measured
atrazine
concentrations,
which
were
determined
four
weeks
before
frogs
were
collected
and
averaged
over
November
2001
to
June
2002
(
Table
2),
indicated
that
atrazine
in
reference
sites
was
generally
an
order
of
magnitude
lower
than
sites
located
in
corn­
growing
areas.
However,
all
concentrations
were
apparently
readily
quantified.

Table
1.
Concentrations
of
pesticides
(

g/
L)
at
the
different
sampling
sites.
Values
were
calculated
from
two
individual
samplings
that
were
conducted
with
a
time
period
in
2002
of
4
weeks
before
collecting
X.

laevis.

Site
C1
C3
C6
E1
E3
E4
E6
E8
4/
2
4/
15
3/
18
4/
2
5/
1
5/
13
4/
15
5/
1
4/
1
4/
15
4/
2
4/
15
4/
15
5/
1
4/
15
5/
1
Simazin
e
<
0.5
<
0.5
<
0.5
<
0.5
<
0.5
<
0.5
<
0.5
<
0.5
<
0.5
<
0.5
<
0.5
<
0.5
<
0.5
<
0.5
1.7
1.2
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Evaluation
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DACT
3.91
<
0.1
<
0.1
<
0.1
<
0.1
<
0.1
<
0.1
<
0.1
0.38
<
0.1
<
0.1
<
0.1
5.45
<
0.1
1.24
<
0.1
DIA
0.23
0.27
0.3
0.61
0.13
0.15
0.7
0.93
0.47
0.28
0.18
0.64
0.49
0.32
0.7
0.88
DEA
0.18
<
0.1
0.15
<
0.1
0.12
0.13
1.29
1.16
0.43
0.38
0.11
<
0.1
1.09
0.9
1.04
0.91
Atrazine
0.28
0.26
0.36
0.57
<
0.1
0.2
4.14
3.5
1.05
1.01
0.32
0.29
3.78
3.9
3.53
3.12
Terbuty
l
<
0.1
<
0.1
<
0.1
<
0.1
0.51
0.59
3.66
2.95
0.97
1.07
0.19
0.24
2.46
2.4
0.86
0.72
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Table
2.
Mean
concentrations
of
atrazine
at
the
sampling
locations
measured
during
four
weeks
collecting
frogs,
and
time
weighted
means
of
atrazine
measured
between
November
2001
and
June
2002.

Values
listed
in
ascending
order
of
concentration
Location
Mean
Atrazine
Concentration
4
weeks
Before
Sampling
Location
Time­
weighted
Mean
Atrazine
Concentration
C6
0.13
C1
0.15
C1
0.27
C6
0.19
E4
0.31
C3
0.25
C3
0.47
E4
0.48
E3
1.0
E3
0.82
E8
3.3
E6
2.6
E1
3.8
E1
3.2
E6
3.8
E8
3.4
Gonadosomatic
Index
(
GSI)

The
means
of
the
median
GSI
values
for
both
female
(
t­
test;
p
=
0.0640)
and
male
(
t­
test;
p
=
0.0725)
from
corn
growing
areas
were
greater
than
the
female
and
male
means
from
the
non­
corn
growing
areas.
The
GSI
of
females
form
E1
and
E8
were
more
variable
than
those
at
other
locations.

Condition
Index
No
statistically
significant
differences
were
observed
in
the
means
of
the
site­
specific
CI
values
between
the
corn­
growing
and
non­
corn
growing
sites
regardless
of
whether
the
analysis
was
based
on
means
or
medians.

Testosterone
(
T)
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Plasma
T
concentrations
were
greater
in
males
than
in
females
except
in
reference
pond
C1
where
mean
male
plasma
testosterone
(

105
pg/
mL)
was
roughly
an
order
of
magnitude
lower
than
females'
mean
plasma
testosterone
(

1005
pg/
mL).
At
sites
C6
and
E6,
males
and
females
contained
roughly
similar
amounts
of
plasma
testosterone.
For
males,
there
was
no
significant
difference
between
plasma
T
in
frogs
from
corngrowing
and
non­
corn
growing
sites,
and
there
was
no
correlation
between
plasma
T
and
atrazine.
However,

there
was
a
significant
negative
correlation
between
the
log
of
male
plasma
T
concentration
and
the
log
of
the
DACT
concentration
(
r=
­
0.839,
p
=
0.009)
Median
and
mean
plasma
T
concentrations
of
males
were
not
significantly
different
between
the
experimental
and
reference
sites.
However,
when
the
sites
were
grouped
into
either
those
with
the
four
greatest
and
four
least
atrazine
concentrations
or
into
groups
where
atrazine
concentrations
were
greater
than
3

g/
L
vs
those
with
concentrations
that
were
less
than
1

g/
L,
there
were
significant
differences,
with
the
frogs
from
the
greater
atrazine
sites
having
less
median
concentrations
of
plasma
T.

Although
the
report
states
that
mean
and
median
female
plasma
testosterone
levels
were
significantly
higher
in
experimental
ponds
than
at
reference
sites
(
t­
test,
mean
p
=
0.018;
median
0.0061),
the
figure
(
Figure
4)

depicting
this
relationship
suggests
the
opposite,
i.
e.,
that
median
female
plasma
testosterone
concentrations
were
higher
in
reference
sites.
This
would
better
support
the
fact
that
female
plasma
T
concentrations
were
negatively
correlated
with
atrazine
concentrations
(
r
=
­
0.725;
p
=
0.042).

Estradiol
(
E2)

Median
female
plasma
concentrations
of
E2
from
experimental
sites
were
significantly
less
than
those
of
females
from
reference
sites
(
t­
test;
p
=
0.0018),
but
there
was
no
significant
difference
for
males.
Mean
plasma
estradiol
concentrations
were
less
at
experimental
sites
for
both
males
(
t­
test,
p
=
0.0186)
and
females
(
t­
test;
p
=
0.0052).
Concentrations
of
E2
in
the
plasma
of
both
males
and
females
were
more
variable
in
reference
sites
than
those
from
experimental
sites.
Male
plasma
E2
were
not
related
to
atrazine
but
were
negatively
correlated
with
the
log
of
DACT
concentrations
(
r
=
­
0.779;
p
=
0.023).
In
females,
plasma
E2
was
negatively
correlated
with
atrazine
concentration
(
r
=
­
0.833;
p
=
0.010).
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E2/
T
Ratio
Because
of
the
magnitude
of
variability
associated
with
this
index,
E2/
T
ratios
for
both
males
and
females
were
not
significantly
different
either
between
the
experimental
and
reference
sites
or
between
other
location
groupings
regardless
of
whether
they
were
based
on
means
or
medians.
E2/
T
ratio
in
males
was
negatively
correlated
with
DIA
concentrations
(
r
=
­
0.830;
p
=
0.011).
There
were
no
significant
relationships
for
E2/
T
ratio
for
females
and
any
triazine
residues.

Aromatase
Activity
Aromatase
levels
in
testes
were
not
measurable
at
most
sites.
There
were
no
significant
differences
in
median
aromatase
activity
in
ovaries
between
experimental
and
reference
or
when
locations
were
grouped
based
on
the
highest
and
lowest
atrazine
concentrations.
The
greatest
variability
in
ovarian
aromatase
activity
was
at
site
E8
and
was
attributed
to
the
extended
sampling
period
(
April
to
September).
Log
ovarian
aromatase
activity
was
correlated
with
the
log
of
the
4WM
water
temperature
(
7
=
0.786;
p
=
0.021);
aromatase
activity
was
not
correlated
with
atrazine
concentration
but
was
correlated
with
with
the
log
of
the
DIA
concentration
(
r
=

0.857;
p
=
0.007).

There
were
statistically
significant
linear
regressions
between
the
logarithms
of
T
and
E2
in
both
males
(
r
=

0.757;
p
<
0.001)
and
females
(
r
=
0.868;
p
<
0.001).
Both
sexes
also
exhibited
positive
relationships
between
log
E2
and
log
E2/
T
(
males:
r
=
0.804,
p
<
0.001;
females:
r
=
0.582,
p
<
0.001).
However,

aromatase
in
females
was
not
related
to
sex
steroid
concentrations.

C.
REPORTED
STATISTICS:
Data
used
in
statistical
tests
were
stratified
by
sex,
i.
e.,
male
and
female
data
analyzed
separately.
Studies
were
designed
to
be
analyzed
by
both
fixed­
effects
models
and
by
regression
type
statistics
because
specific
locations
could
not
be
classified
as
exposed
or
unexposed
since
atrazine
residues
were
also
detected
in
non­
corn
growing
"
reference
sites".
Statistical
comparisons
were
made
between
the
median
concentrations
of
plasma
testosterone
and
estradiol
as
well
as
the
E2/
T
ratio,
the
GSI,
the
EI
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gonadal
aromatase;
regression
analysis
between
atrazine
concentration
and
these
parameters
was
also
conducted..
For
statistical
tests,
locations
were
grouped
several
ways:
grouped
based
on
whether
they
were
in
corn­
growing
(
E)
or
non­
corn
growing
(
C)
areas;
grouped
with
a
mean
atrazine
concentration

1.0

g/
L
(
C1,
C3,
C6,
E3,
E4)
versus
>
3

g/
L
(
E1,
E6,
E8).
Finally,
study
sites
were
grouped
with
the
four
sites
with
the
4
smallest
means
in
one
group,
and
the
remaining
four
sites
with
the
four
largest
means
in
the
other
group.

Two­
sided
two
sample
t­
test
was
used
to
compare
the
mean
of
the
site
values
in
the
first
group
to
the
mean
of
the
site
values
in
the
second
group.
Two­
sample
Kolmogorov­
Smirnov
test
was
used
to
compare
individual
locations.
Linear
model
comparisons
of
four
­
week
average
atrazine
and/
or
metabolites
were
used
for
each
parameter.

D.
VERIFICATION
OF
STATISTICAL
RESULTS:
See
attached
SAS
©
output
(
Statistical
Analysis
System,
Release
8.01,
Cary,
North
Carolina.

;

E.
STUDY
DEFICIENCIES:

Atrazine
and/
or
triazine
degradates
were
present
in
control
sites.

While
the
low
sample
size
at
sites
E1
and
E8
may
have
been
explained
by
the
sudden
introduction
of
predatory
catfish,
it
does
not
explain
why
the
remaining
corn­
growing
sites
(
E3,
E4
and
E6)
also
yielded
fewer
frogs
than
non­
corn
growing
sites.

Because
frogs
were
sampled
over
disproportionate
periods
of
time,
e.
g.,
6
months
for
site
E8,
samples
would
likely
contain
animals
at
different
stages
of
development
Frog
traps
were
baited
with
liver/
meat
scraps
that
may
have
contained
hormones.

Following
capture,
frogs
were
housed
individually
in
"
clean
water"
for
48
hours.
The
volume
and
characteristics
of
this
water
were
not
described
in
the
study.
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F.
REVIEWER'S
COMMENTS:

The
large
amount
of
variability
associated
with
aromatase
and
plasma
hormone
data
in
laboratory
studies
make
it
difficult
to
understand
the
utility
of
conducting
a
field
study
with
these
parameters.
Collecting
animals
over
a
six­
month
period
would
also
contribute
to
extremely
high
variability.

Atrazine
is
typically
applied
in
October
/
November
when
corn
is
planted
in
South
Africa.
Frog
sampling
was
conducted
after
the
rainy
season
in
April
to
May
and
water
samples
to
occurred
within
a
time
span
of
four
weeks
before
frogs
were
sampled.
At
exposure
site
E8,
frogs
were
sampled
over
a
6­
month
time
frame.
The
fewer
number
of
frogs
was
attributed
to
a
high
runoff
event
that
washed
catfish
into
the
pond;
the
catfish
preyed
on
the
frogs.

Reference
and
atrazine­
exposure
sites
appeared
to
be
widely
divergent,
ranging
from
5.1
­
8.8
at
reference
sites
and
7.2
­
10.8
at
atrazine
sites.

Frogs
were
attracted
into
baited
traps
containing
liver
and/
or
scrap
meat
which
may
have
contained
hormones.

Apparently
trapped
animals
were
held
in
cages
for
two
days
which
should
have
provided
sufficient
time
to
consume
all
of
the
bait.
Also,
depending
on
catch
rates,
the
loading
within
the
cages
could
have
stressed
the
frogs.
Although
it
doesn't
state
the
desired
sample
size
in
the
report's
methodology,
apparently
(
based
on
the
raw
data),
20
male
and
20
female
frogs
were
to
be
sampled
at
each
site.
Table
3
shows
that
while
the
desired
sample
size
was
generally
available
for
frogs
collected
in
non­
corn
growing
areas,
sample
sizes
were
minimally
50%
lower
in
corn­
growing
areas.
In
site
E1,
sampling
for
female
frogs
extended
into
late
June,
and
at
site
E8
sampling
extended
into
mid­
August
for
females
and
into
mid­
September
for
males.
Extended
sampling
periods
at
these
two
sites
may
have
resulted
in
collecting
animals
that
were
at
considerably
different
states
of
development.
The
gonadosomatic
index
and
ovarian
aromatase
activity
in
females
collected
from
sites
E1
and
E8
expressed
the
highest
variability
relative
to
any
of
the
other
treatment
sites.

Table
3.
Summary
of
total
number
of
Xenopus
laevis
collected
from
April
through
May
2002
at
non­
corn
growing
(
C)
and
corn
growing
(
E)
sites
in
South
Africa.
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Site
Males
Females
C1
20
(
Apr
21
­
Apr
23)
20
(
Apr
21
­
May
1)

C3
17
(
Apr
4
­
Apr
11)
20
(
Apr
4
­
Apr
11)

C6
20
(
May
13
­
May
15)
20
(
May
13
­
May
14)

E1
8
(
April
29
­
May
7)
8
(
April
29
­
June
29)

E3
10
(
Apr
9
­
Apr
17)
10
(
Apr
9
­
Apr
18)

E4
10
(
Apr
13
­
Apr
15)
10
(
Apr
14
­
Apr
16)

E6
10
(
May
5
­
May
9)
10
(
May
5
­
May
6)

E8
6
(
Apr
29
­
Sep
17)
15
(
Apr
29
­
Aug
17)

Statistical
analyses
were
more
regression­
based
because
specific
sampling
locations
could
not
be
classified
as
exposed
or
unexposed.
There
was
a
range
of
concentrations
of
primary
residues
of
concern,
with
greater
concentrations
in
the
corn­
growing
areas;
however,
residues
were
also
detected
in
non­
corn
growing
areas.

In
Phase
II
of
the
study
(
Smith
et
al.
2003),
the
authors
suggested
that
atrazine
residues
in
reference
ponds
may
be
a
result
of
atmospheric
deposition
(
wind).

The
GSI
and
aromatase
ovarian
activity
of
females
from
E8
were
more
variable
than
those
at
other
locations.

This
variability
may
have
resulted
from
the
long
sampling
period
(
six
months)
and
the
considerable
changes
in
the
frogs'
reproductive
state.

Gonadosomatic
index
suggests
that
both
males
and
females
collected
in
corn­
growing
areas
were
more
sexually
developed
than
their
counterparts
in
"
reference"
sites.

Comparisons
between
male
and
female
plasma
testosterone
levels
were
also
are
hindered
by
the
considerable
variability
within
pond
and
within
treatment
groups.
Although
median
plasma
testosterone
levels
were
qualitatively
different.
Males
in
reference
ponds
had
higher
median
plasma
testosterone
levels
than
males
in
experimental
ponds
by
approximately
a
factor
of
2.
Similarly,
females
in
reference
ponds
had
higher
median
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plasma
testosterone
levels
than
females
in
experimental
ponds
by
approximately
a
factor
of
8.
However,

given
the
magnitude
of
variability,
it
wasn't
possible
to
document
statistical
differences
in
means
or
medians.

Only
when
the
samples
were
grouped
based
on
the
three
highest
and
three
lowest
atrazine
exposures
could
statistical
differences
be
documented
in
median
T
levels.
Also,
the
text
may
be
in
error
when
it
concludes
that
female
plasma
testosterone
levels
were
higher
in
corn­
growing
areas
(
experimental)
than
in
non­
corn
growing
areas
because
the
statement
appears
to
contradict
the
figure
(
Figure
4)
depicting
the
data
and
from
the
significant
correlation
showing
that
female
plasma
testosterone
is
negatively
related
to
atrazine.

Based
on
Figure
5
,
median
plasma
E2
concentrations
in
males
from
reference
sites
were
approximately
similar
to
female
plasma
estradiol
concentrations
in
reference
sites,
while
median
male
plasma
estradiol
at
reference
sites
was
approximately
five­
fold
higher
than
plasma
estradiol
concentrations
of
males
from
experimental
sites.

A
similar
pattern
existed
for
females
where
reference
site
animals
had
roughly
six­
fold
higher
estradiol
concentrations
than
females
at
experimental
sites.

Although
the
study
authors
conclude
that
there
were
no
statistical
differences
in
the
ratio
of
estradiol
to
testosterone
(
E2/
T),
in
a
qualitative
sense,
the
median
values
for
females
were
relatively
consistent
across
experimental
and
reference
sites.
However,
the
median
ratio
for
males
tended
to
be
roughly
double
in
animals
collected
from
reference
sites
compared
to
those
collected
at
experimental
sites.

Although
not
considered
very
predictive
(
correlation
coefficients
ranged
from
0.22
to
0.38),
there
were
significant
correlations
(
p<
0.026)
between
condition
index
and
both
gonadosomatic
index
and
log
aromatase
activity
in
females.
This
correlation
is
intuitively
obvious
because
more
gravid
females
should
weigh
more
and
have
higher
aromatase
activity
than
less
developed
animals.

The
report
discussed
a
number
of
statistical
differences
that
were
detected
and
how
misleading
this
could
be
based
on
the
number
of
statistical
comparisons
that
were
run.
The
authors
suggested
that
if
Bonferroni's
correction
had
been
made,
none
of
the
p
values
would
have
been
significant.
The
Bonferroni
correction
is
used
to
control
experiment
error
rates
and
is
obtained
by
dividing
the
overall
 ­
level
for
the
study
by
the
number
of
groups
being
compared.
If
the
study
has
a
large
number
of
means,
however,
this
process
can
be
very
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conservative.
It
may
also
fail
to
detect
significant
differences
between
groups
and
increase
the
likelihood
of
making
a
Type
II
error.

In
this
study,
the
authors
discuss
the
relevancy
of
the
Tavera­
Mendoza
et
al.
(
2002a
and
2002b)
studies
which
showed
decreased
testicular
volume
in
animals
exposed
to
21

g/
L
and
how
the
current
study
demonstrated
increases
in
GSI.
At
the
same
time,
though,
they
note
that
the
current
study's
maximum
exposure
values
were
roughly
five­
fold
lower
than
those
used
in
the
Tavera­
Mendoza
study
and
that
improved
food
sources
may
have
resulted
in
increased
gonadal
weight
.
Others
differences
in
the
Tavera­
Mendoza
study
include:
the
laboratory
study
was
presumably
conducted
on
animals
that
were
probably
in
the
same
state
of
development,
the
environmental
conditions
in
the
laboratory
were
better
defined,
and
the
laboratory
study
was
an
acute
exposure
(
48
hour)
as
opposed
to
the
current
chronic
study.

The
authors
state
that
there
is
no
plausible
mechanism
that
could
be
postulated
to
explain
the
negative
correlation
between
plasma
E2
concentrations
in
females
and
concentrations
of
atrazine
in
pond
water;

however,
they
note
that
correlations
do
not
imply
causality.

Since
there
were
no
differences
in
aromatase
activities
between
locations
or
significant
correlations
between
exposure
to
atrazine
and
aromatase
activity,
the
authors
believe
that
these
findings
do
not
support
the
Hayes
et
al.
2002a,
b
hypothesis
that
atrazine
causes
an
increase
in
the
production
of
estrogen
by
inducing
aromatase
activity.
The
fact
that
plasma
T
concentrations
were
less
in
groups
with
the
higher
atrazine
exposure
values
was
viewed
as
being
consistent
with
the
hypothesis
that
atrazine
decreased
plasma
T,
but
the
fact
that
atrazine
concentrations
were
not
correlated
with
plasma
T
was
viewed
as
suggesting
that
the
statistical
significance
was
artifactual.
The
significant
negative
correlation
between
the
E2/
T
ratio
of
males
and
DIA
was
viewed
as
being
inconsistent
with
Sanderson
et
al.
2001,
showing
that
DIA
significantly
increased
CYP­
19
mRNA
expression
as
well
as
aromatase
activity.
They
concluded,
though,
that
the
differences
in
plasma
steroid
hormone
concentrations
were
not
caused
by
up­
regulation
of
aromatase
activity.
The
report
states
"
although
significant
negative
correlations
were
observed
between
exposure
to
atrazine
and
its
metabolite
DACT
and
plasma
concentrations
of
sex
steroids
in
wild
X.
laevis,
it
is
impossible
from
this
study
to
conclude
the
exact
cause
or
accuracy
of
these
differences."
The
experimental
corn­
growing
regions
were
subject
to
a
range
of
chemicals
there
were
not
fully
characterized
and
that
may
have
contributed
to
the
observed
statistical
differences.
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G.
CONCLUSIONS:

This
study
indicates
that
plasma
hormone
levels
and
gonadal
aromatase
activity
are
highly
variable
and
depend
on
a
number
of
factors
that
were
not
anticipated
when
the
study
was
designed.
Reference
ponds
in
areas
of
South
Africa
where
corn
is
not
grown
were
subject
to
variable
levels
of
atrazine
contamination
that
approached
exposure
values
for
ponds
in
corn­
growing
regions.
Collecting
frogs
over
protracted
periods
of
time
may
have
constituted
one
of
the
greatest
sources
of
variability
because
the
frogs
were
probably
at
different
stages
of
their
sexual
cycles.
It
is
unclear
what
effect
the
bait
used
to
attract
frogs
into
traps
may
have
had
on
hormone
levels.
In
spite
of
the
fact
that
in
some
comparisons
the
study
was
unable
to
differentiate
male
and
female
hormone
levels,
some
evidence
suggested
that
males
subjected
to
higher
atrazine
concentrations
tended
to
have
reduced
plasma
testosterone
and
that
higher
concentrations
of
the
atrazine
degradate
DACT
were
associated
with
reduced
plasma
testosterone
levels.
Also,
contrary
to
what
the
authors
state,
median
female
plasma
testosterone
appeared
to
be
significantly
higher
in
reference
sites
where
atrazine
exposure
was
reduced.
This
conclusion
is
consistent
with
the
authors'
observation
that
female
plasma
testosterone
was
negatively
correlated
with
atrazine
concentration.
Mean
plasma
estradiol
concentrations
in
both
males
and
females
were
significantly
lower
in
high
atrazine
exposure
sites,
and
like
testosterone,
male
estradiol
concentrations
were
negatively
correlated
with
concentrations
of
the
atrazine
degradate
DACT.
Female
estradiol
concentrations
were
negatively
correlated
with
atrazine
concentrations.
Gonadal
aromatase
activity
in
males
could
not
be
accurately
characterized
because
of
its
low
level
across
the
majority
of
collection
sites,

while
high
variability
in
female
aromatase
activity
made
all
the
comparisons
insignificant.
Aromatase
activity,

though,
was
negatively
correlated
with
concentrations
of
the
atrazine
degradate
DIA.

Ideally,
a
study
should
be
designed
based
on
the
variability
associated
with
the
measurement
endpoints.

Sample
sizes
should
reflect
the
number
of
test
animals
required
to
identify
a
specified
difference
within
a
given
level
of
certainty.
Potential
sources
of
variability
should
also
be
identified
and
controlled
to
the
extent
possible.

The
current
study
did
not
appear
to
base
its
design
on
the
variability
associated
with
the
range
of
measurement
endpoints.
Animals
were
collected
over
broad
periods
of
time
(
up
to
six
months)
which
would
assure
variation
within
the
same
group
in
terms
of
development.
Based
on
the
number
of
animals
collected,
frogs
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appeared
to
be
more
abundant
in
reference
sites
than
in
corn­
growing
regions
where
half
as
many
frogs
were
collected.
The
entire
design
was
questionable,
including:
the
extended
sampling
periods
in
ponds
of
questionable
similarity,
baiting
with
potentially
hormone­
laden
liver/
meat,
and
confinement
in
cages
at
unknown
loading
rates.
These
factors
could
confound
the
study's
ability
to
reliably
and
reasonably
quantify
"
typical"
steroid
hormone
levels
and/
or
aromatase
activity
in
Xenopus.
Additionally,
the
presence
of
atrazine
and
triazine
degradates
across
all
sites
(
reference
and
corn­
growing)
and
the
failure
to
characterize
other
pesticides
at
the
study
sites
limits
the
usefulness
of
this
study
in
determining
potential
effects
of
atrazine
and
in
supporting
the
study's
hypothesis.

Before
a
field
study
of
this
scope
is
undertaken,
researchers
need
to
conduct
a
pilot
study
to
establish
the
necessary
sample
size
for
detecting
a
specified
difference
in
the
biological
attribute
being
measured
within
a
certain
level
of
confidence.
The
sample
size
would
be
based
on
the
variability
associated
with
a
particular
parameter.
Unfortunately,
the
levels
of
variability
associated
with
the
parameters
of
interest
in
this
study
would
likely
require
very
high
sample
sizes.
A
more
practical
approach
would
be
to
identify
major
sources
of
variability
and
then
try
to
control
those
conditions.

The
current
data
suggest
that
atrazine
and/
or
its
degradates
may
be
impacting
plasma
testosterone;
however,

the
data
are
not
conclusive
for
the
reasons
cited
above.

H.
REFERENCES:

Cuisset,
G.,
P.
Pradelles,
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E.
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ketotestosterone
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Hayes,
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B.,
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S.
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Hermaphroditic,
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Hayes,
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Environmental
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Kennedy,
S.
W.
and
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P.
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Simultaneous
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Laphart,
E.
D.
and
E.
R.
Simpson.
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Assay
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Sanderson,
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T.,
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Seinen,
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P.
Giesy
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van
den
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2000.
2­
chloro­
S­
triazine
herbicides
induce
aromatase
activity
in
H295R
human
adreno
cortical
carcinoma
cells.
A
novel
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for
estrogenicity.

Toxicol.
Sci.,
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Sanderson,
J.
t.,
R.
J.
Letcher,
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Heneweer,
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P.
Giesy,
M.
van
den
Berg.
2001.
Effects
of
chloro­
S­
triazine
herbicides
and
metabolites
on
aromatase
activity
in
various
human
cell
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and
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Smith,
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2003.
Field
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laevis
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exposure
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gonadal
responses.
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&
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Texas
79490
(
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and
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Potchefstroom
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of
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Tavera­
Mendoza,
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Ruby,
P.
Brousseau,
M.
Fournier,
D.
Cyr
and
D.
Marcogliese.
2001.
Response
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ovary.
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Tavera­
Mendoza,
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D.
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on
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Analytical
Support
 
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152
MEAN
LENGTHS
OF
FROGS
COLLECTED
BY
POND
AND
SEX
391
Obs
POND
Sex
GROUP
_
TYPE_
_
FREQ_
LENGTH
L_
SD
L_
CV
1
C1
F
REF
0
20
69.5450
8.0156
11.5258
2
C1
M
REF
0
20
58.0000
6.6373
11.4436
3
C3
F
REF
0
20
68.9100
14.4936
21.0326
4
C3
M
REF
0
20
56.9882
10.9170
19.1565
5
C6
F
REF
0
20
76.9500
7.0433
9.1531
6
C6
M
REF
0
20
72.1100
7.1908
9.9721
7
E1
F
EXP
0
10
64.4833
14.3272
22.2185
8
E1
M
EXP
0
10
59.2000
9.4870
16.0253
9
E3
F
EXP
0
10
74.3900
6.9437
9.3341
10
E3
M
EXP
0
10
66.6300
6.9863
10.4853
11
E4
F
EXP
0
10
77.5400
10.9218
14.0853
12
E4
M
EXP
0
10
63.8300
3.3549
5.2561
13
E6
F
EXP
0
10
88.5000
9.5274
10.7654
14
E6
M
EXP
0
10
62.6600
9.5755
15.2816
15
E8
F
EXP
0
15
64.4733
13.8195
21.4344
16
E8
M
EXP
0
10
60.7167
7.2841
11.9968
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ANOVA
FOR
LENGTH
OF
FROGS
BETWEEN
PONDS
BY
SEX
392
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
POND
8
C1
C3
C6
E1
E3
E4
E6
E8
Number
of
observations
115
NOTE:
Due
to
missing
values,
only
111
observations
can
be
used
in
this
analysis.

Dependent
Variable:
LENGTH
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
7
5026.98373
718.14053
6.07
<.
0001
Error
103
12178.98817
118.24260
Corrected
Total
110
17205.97189
R­
Square
Coeff
Var
Root
MSE
LENGTH
Mean
0.292165
14.96340
10.87394
72.67027
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
POND
7
5026.983725
718.140532
6.07
<.
0001
Levene's
Test
for
Homogeneity
of
LENGTH
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
POND
7
432098
61728.3
3.51
0.0020
Error
103
1810469
17577.4
Bartlett's
Test
for
Homogeneity
of
LENGTH
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
POND
7
17.4523
0.0147
Dunnett's
t
Tests
for
LENGTH
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.
Data
Evaluation
Report
on
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Number
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01
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24
of
152
Alpha
0.05
Error
Degrees
of
Freedom
103
Error
Mean
Square
118.2426
Critical
Value
of
Dunnett's
t
2.69261
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
POND
Between
Simultaneous
95%

Comparison
Means
Confidence
Limits
E6
­
C1
18.955
7.615
30.295
***

E4
­
C1
7.995
­
3.345
19.335
C6
­
C1
7.405
­
1.854
16.664
E3
­
C1
4.845
­
6.495
16.185
C3
­
C1
­
0.635
­
9.894
8.624
E1
­
C1
­
5.062
­
18.690
8.567
E8
­
C1
­
5.072
­
15.072
4.929
Data
Evaluation
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25
of
152
ANOVA
FOR
LENGTH
OF
FROGS
BETWEEN
PONDS
BY
SEX
396
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
POND
8
C1
C3
C6
E1
E3
E4
E6
E8
Number
of
observations
110
NOTE:
Due
to
missing
values,
only
101
observations
can
be
used
in
this
analysis.

Dependent
Variable:
LENGTH
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
7
3055.667841
436.523977
6.78
<.
0001
Error
93
5987.449980
64.381183
Corrected
Total
100
9043.117822
R­
Square
Coeff
Var
Root
MSE
LENGTH
Mean
0.337900
12.78217
8.023789
62.77327
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
POND
7
3055.667841
436.523977
6.78
<.
0001
Levene's
Test
for
Homogeneity
of
LENGTH
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
POND
7
92002.7
13143.2
2.03
0.0591
Error
93
601693
6469.8
Bartlett's
Test
for
Homogeneity
of
LENGTH
Variance
Data
Evaluation
Report
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EPA
MRID
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Source
DF
Chi­
Square
Pr
>
ChiSq
POND
7
14.5701
0.0419
Dunnett's
t
Tests
for
LENGTH
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
93
Error
Mean
Square
64.38118
Critical
Value
of
Dunnett's
t
2.70384
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
POND
Between
Simultaneous
95%

Comparison
Means
Confidence
Limits
C6
­
C1
14.110
7.249
20.971
***

E3
­
C1
8.630
0.228
17.032
***

E4
­
C1
5.830
­
2.572
14.232
E6
­
C1
4.660
­
3.742
13.062
E8
­
C1
2.717
­
7.382
12.815
E1
­
C1
1.200
­
7.876
10.276
C3
­
C1
­
1.012
­
8.169
6.145
Data
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NONPARAMETRIC
COMPARISON
OF
FROG
LENGTH
BETWEEN
PONDS
400
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
LENGTH
Classified
by
Variable
POND
Sum
of
Expected
Std
Dev
Mean
POND
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C1
20
896.00
1120.0
130.323757
44.800000
C3
20
975.50
1120.0
130.323757
48.775000
C6
20
1361.00
1120.0
130.323757
68.050000
E1
6
209.00
336.0
76.675775
34.833333
E3
10
593.00
560.0
97.084207
59.300000
E4
10
656.00
560.0
97.084207
65.600000
E6
10
945.50
560.0
97.084207
94.550000
E8
15
580.00
840.0
115.922875
38.666667
Average
scores
were
used
for
ties.

Kruskal­
Wallis
Test
Chi­
Square
28.5206
DF
7
Pr
>
Chi­
Square
0.0002
The
NPAR1WAY
Procedure
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
LENGTH
Classified
by
Variable
POND
Sum
of
Expected
Std
Dev
Mean
POND
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C1
20
7.0
9.909910
2.033723
0.350000
C3
20
6.0
9.909910
2.033723
0.300000
C6
20
14.0
9.909910
2.033723
0.700000
E1
6
1.0
2.972973
1.196537
0.166667
E3
10
6.0
4.954955
1.515014
0.600000
E4
10
5.0
4.954955
1.515014
0.500000
E6
10
10.0
4.954955
1.515014
1.000000
E8
15
6.0
7.432432
1.808995
0.400000
Average
scores
were
used
for
ties.
Data
Evaluation
Report
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EPA
MRID
Number
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28
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152
Median
One­
Way
Analysis
Chi­
Square
21.6625
DF
7
Pr
>
Chi­
Square
0.0029
Data
Evaluation
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EPA
MRID
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NONPARAMETRIC
COMPARISON
OF
FROG
LENGTH
BETWEEN
PONDS
403
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

Wilcoxon
Scores
(
Rank
Sums)
for
Variable
LENGTH
Classified
by
Variable
POND
Sum
of
Expected
Std
Dev
Mean
POND
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C1
20
713.00
1020.0
117.339834
35.650000
C3
17
559.00
867.0
110.167138
32.882353
C6
20
1600.50
1020.0
117.339834
80.025000
E1
8
312.50
408.0
79.519644
39.062500
E3
10
644.50
510.0
87.944495
64.450000
E4
10
552.50
510.0
87.944495
55.250000
E6
10
494.00
510.0
87.944495
49.400000
E8
6
275.00
306.0
69.602588
45.833333
Average
scores
were
used
for
ties.

Kruskal­
Wallis
Test
Chi­
Square
35.4807
DF
7
Pr
>
Chi­
Square
<.
0001
The
NPAR1WAY
Procedure
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
LENGTH
Classified
by
Variable
POND
Sum
of
Expected
Std
Dev
Mean
POND
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C1
20
6.0
9.900990
2.012363
0.300000
C3
17
3.0
8.415842
1.889352
0.176471
C6
20
20.0
9.900990
2.012363
1.000000
E1
8
2.0
3.960396
1.363751
0.250000
E3
10
6.0
4.950495
1.508236
0.600000
E4
10
6.0
4.950495
1.508236
0.600000
E6
10
4.0
4.950495
1.508236
0.400000
E8
6
3.0
2.970297
1.193675
0.500000
Average
scores
were
used
for
ties.
Data
Evaluation
Report
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EPA
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Number
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Median
One­
Way
Analysis
Chi­
Square
33.1792
DF
7
Pr
>
Chi­
Square
<.
0001
Data
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EPA
MRID
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Page
31
of
152
ANOVA
FOR
LENGTH
OF
FROGS
BETWEEN
REFERENCE
(
REF)
AND
EXPERIMENTAL
(
EXP)
SITES
404
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
GROUP
2
EXP
REF
Number
of
observations
8
Dependent
Variable:
LENGTH
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
8.0782352
8.0782352
0.11
0.7524
Error
6
444.1424033
74.0237339
Corrected
Total
7
452.2206385
R­
Square
Coeff
Var
Root
MSE
LENGTH
Mean
0.017863
11.76994
8.603705
73.09896
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
GROUP
1
8.07823521
8.07823521
0.11
0.7524
Levene's
Test
for
Homogeneity
of
LENGTH
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
GROUP
1
8547.2
8547.2
1.76
0.2326
Error
6
29101.8
4850.3
Bartlett's
Test
for
Homogeneity
of
LENGTH
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
GROUP
1
1.1508
0.2834
Dunnett's
t
Tests
for
LENGTH
Data
Evaluation
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EPA
MRID
Number
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01
Page
32
of
152
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
6
Error
Mean
Square
74.02373
Critical
Value
of
Dunnett's
t
2.44695
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
Simultaneous
GROUP
Between
95%
Confidence
Comparison
Means
Limits
REF
­
EXP
­
2.076
­
17.451
13.299
Data
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Number
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ANOVA
FOR
LENGTH
OF
FROGS
BETWEEN
REFERENCE
(
REF)
AND
EXPERIMENTAL
(
EXP)
SITES
408
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

Class
Level
Information
Class
Levels
Values
GROUP
2
EXP
REF
Number
of
observations
8
Dependent
Variable:
LENGTH
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
0.1091324
0.1091324
0.00
0.9533
Error
6
175.7919208
29.2986535
Corrected
Total
7
175.9010531
R­
Square
Coeff
Var
Root
MSE
LENGTH
Mean
0.000620
8.658181
5.412823
62.51686
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
GROUP
1
0.10913236
0.10913236
0.00
0.9533
Levene's
Test
for
Homogeneity
of
LENGTH
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
GROUP
1
3162.6
3162.6
5.27
0.0615
Error
6
3600.4
600.1
Bartlett's
Test
for
Homogeneity
of
LENGTH
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
GROUP
1
2.7649
0.0964
Dunnett's
t
Tests
for
LENGTH
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
Data
Evaluation
Report
on
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Analysis
EPA
MRID
Number
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Page
34
of
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against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
6
Error
Mean
Square
29.29865
Critical
Value
of
Dunnett's
t
2.44695
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
GROUP
Between
Simultaneous
95%

Comparison
Means
Confidence
Limits
REF
­
EXP
­
0.2413
­
9.9140
9.4315
Data
Evaluation
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EPA
MRID
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NONPARAMETRIC
COMPARISON
OF
FROG
LENGTH
BETWEEN
REFERENCE
AND
EXPERIMENTAL
SITES
412
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
LENGTH
Classified
by
Variable
GROUP
Sum
of
Expected
Std
Dev
Mean
GROUP
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
EXP
5
23.0
22.50
3.354102
4.600000
REF
3
13.0
13.50
3.354102
4.333333
Wilcoxon
Two­
Sample
Test
Statistic
13.0000
Normal
Approximation
Z
0.0000
One­
Sided
Pr
<
Z
0.5000
Two­
Sided
Pr
>
|
Z|
1.0000
t
Approximation
One­
Sided
Pr
<
Z
0.5000
Two­
Sided
Pr
>
|
Z|
1.0000
Z
includes
a
continuity
correction
of
0.5.

Kruskal­
Wallis
Test
Chi­
Square
0.0222
DF
1
Pr
>
Chi­
Square
0.8815
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
LENGTH
Classified
by
Variable
GROUP
Sum
of
Expected
Std
Dev
Mean
GROUP
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
EXP
5
3.0
2.50
0.731925
0.600000
REF
3
1.0
1.50
0.731925
0.333333
Data
Evaluation
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Median
Two­
Sample
Test
Statistic
1.0000
Z
­
0.6831
One­
Sided
Pr
<
Z
0.2473
Two­
Sided
Pr
>
|
Z|
0.4945
Median
One­
Way
Analysis
Chi­
Square
0.4667
DF
1
Pr
>
Chi­
Square
0.4945
Data
Evaluation
Report
on
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Hormone
and
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Analysis
EPA
MRID
Number
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01
Page
37
of
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NONPARAMETRIC
COMPARISON
OF
FROG
LENGTH
BETWEEN
REFERENCE
AND
EXPERIMENTAL
SITES
414
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
LENGTH
Classified
by
Variable
GROUP
Sum
of
Expected
Std
Dev
Mean
GROUP
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
EXP
5
25.0
22.50
3.354102
5.000000
REF
3
11.0
13.50
3.354102
3.666667
Wilcoxon
Two­
Sample
Test
Statistic
11.0000
Normal
Approximation
Z
­
0.5963
One­
Sided
Pr
<
Z
0.2755
Two­
Sided
Pr
>
|
Z|
0.5510
t
Approximation
One­
Sided
Pr
<
Z
0.2849
Two­
Sided
Pr
>
|
Z|
0.5698
Z
includes
a
continuity
correction
of
0.5.

Kruskal­
Wallis
Test
Chi­
Square
0.5556
DF
1
Pr
>
Chi­
Square
0.4561
The
NPAR1WAY
Procedure
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
LENGTH
Classified
by
Variable
GROUP
Sum
of
Expected
Std
Dev
Mean
GROUP
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
EXP
5
3.0
2.50
0.731925
0.600000
Data
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REF
3
1.0
1.50
0.731925
0.333333
Median
Two­
Sample
Test
Statistic
1.0000
Z
­
0.6831
One­
Sided
Pr
<
Z
0.2473
Two­
Sided
Pr
>
|
Z|
0.4945
Median
One­
Way
Analysis
Chi­
Square
0.4667
DF
1
Pr
>
Chi­
Square
0.4945
Data
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MEAN
WEIGHTS
OF
FROGS
COLLECTED
BY
POND
AND
SEX
416
Obs
POND
Sex
GROUP
_
TYPE_
_
FREQ_
WEIGHT
W_
SD
W_
CV
1
C1
F
REF
0
20
32.8315
11.1449
33.9459
2
C1
M
REF
0
20
19.4445
6.1022
31.3826
3
C3
F
REF
0
20
35.3455
20.2334
57.2446
4
C3
M
REF
0
20
20.7541
13.2996
64.0818
5
C6
F
REF
0
20
41.2495
10.4261
25.2757
6
C6
M
REF
0
20
37.2590
12.0340
32.2982
7
E1
F
EXP
0
10
29.1450
19.2315
65.9856
8
E1
M
EXP
0
10
23.3888
14.4006
61.5704
9
E3
F
EXP
0
10
39.3810
8.4697
21.5071
10
E3
M
EXP
0
10
32.5740
8.9938
27.6105
11
E4
F
EXP
0
10
48.8210
18.5235
37.9417
12
E4
M
EXP
0
10
29.9000
4.1673
13.9376
13
E6
F
EXP
0
10
66.9560
22.6873
33.8838
14
E6
M
EXP
0
10
26.1050
12.3935
47.4758
15
E8
F
EXP
0
15
29.8047
18.8819
63.3521
16
E8
M
EXP
0
10
23.0917
8.7925
38.0765
Data
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ANOVA
FOR
WEIGHT
OF
FROGS
BETWEEN
PONDS
BY
SEX
417
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
POND
8
C1
C3
C6
E1
E3
E4
E6
E8
Number
of
observations
115
NOTE:
Due
to
missing
values,
only
111
observations
can
be
used
in
this
analysis.

Dependent
Variable:
WEIGHT
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
7
11751.86955
1678.83851
6.31
<.
0001
Error
103
27410.49679
266.12133
Corrected
Total
110
39162.36634
R­
Square
Coeff
Var
Root
MSE
WEIGHT
Mean
0.300081
41.51186
16.31323
39.29775
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
POND
7
11751.86955
1678.83851
6.31
<.
0001
Levene's
Test
for
Homogeneity
of
WEIGHT
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
POND
7
2120258
302894
2.53
0.0190
Error
103
12311095
119525
Bartlett's
Test
for
Homogeneity
of
WEIGHT
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
POND
7
20.4080
0.0048
Dunnett's
t
Tests
for
WEIGHT
Data
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NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
103
Error
Mean
Square
266.1213
Critical
Value
of
Dunnett's
t
2.69261
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
POND
Between
Simultaneous
95%

Comparison
Means
Confidence
Limits
E6
­
C1
34.124
17.112
51.137
***

E4
­
C1
15.990
­
1.023
33.002
C6
­
C1
8.418
­
5.472
22.308
E3
­
C1
6.549
­
10.463
23.562
C3
­
C1
2.514
­
11.376
16.404
E8
­
C1
­
3.027
­
18.030
11.976
E1
­
C1
­
3.687
­
24.133
16.760
Data
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ANOVA
FOR
WEIGHT
OF
FROGS
BETWEEN
PONDS
BY
SEX
421
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
POND
8
C1
C3
C6
E1
E3
E4
E6
E8
Number
of
observations
110
NOTE:
Due
to
missing
values,
only
101
observations
can
be
used
in
this
analysis.

Dependent
Variable:
WEIGHT
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
7
4500.49282
642.92755
5.75
<.
0001
Error
93
10393.96375
111.76305
Corrected
Total
100
14894.45657
R­
Square
Coeff
Var
Root
MSE
WEIGHT
Mean
0.302159
39.57071
10.57180
26.71624
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
POND
7
4500.492823
642.927546
5.75
<.
0001
Levene's
Test
for
Homogeneity
of
WEIGHT
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
POND
7
339909
48558.4
1.40
0.2144
Error
93
3223997
34666.6
Bartlett's
Test
for
Homogeneity
of
WEIGHT
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
Data
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POND
7
22.0595
0.0025
Dunnett's
t
Tests
for
WEIGHT
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
93
Error
Mean
Square
111.7631
Critical
Value
of
Dunnett's
t
2.70384
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
POND
Between
Simultaneous
95%

Comparison
Means
Confidence
Limits
C6
­
C1
17.815
8.775
26.854
***

E3
­
C1
13.130
2.059
24.200
***

E4
­
C1
10.455
­
0.615
21.526
E6
­
C1
6.661
­
4.410
17.731
E1
­
C1
3.944
­
8.013
15.902
E8
­
C1
3.647
­
9.658
16.953
C3
­
C1
1.310
­
8.120
10.739
Data
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NONPARAMETRIC
COMPARISON
OF
FROG
WEIGHT
BETWEEN
PONDS
425
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
WEIGHT
Classified
by
Variable
POND
Sum
of
Expected
Std
Dev
Mean
POND
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C1
20
895.50
1120.0
130.332621
44.775000
C3
20
971.00
1120.0
130.332621
48.550000
C6
20
1266.00
1120.0
130.332621
63.300000
E1
6
215.50
336.0
76.680990
35.916667
E3
10
601.00
560.0
97.090810
60.100000
E4
10
724.00
560.0
97.090810
72.400000
E6
10
950.00
560.0
97.090810
95.000000
E8
15
593.00
840.0
115.930760
39.533333
Average
scores
were
used
for
ties.

Kruskal­
Wallis
Test
Chi­
Square
28.2346
DF
7
Pr
>
Chi­
Square
0.0002
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
WEIGHT
Classified
by
Variable
POND
Sum
of
Expected
Std
Dev
Mean
POND
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C1
20
7.0
9.909910
2.033723
0.350000
C3
20
8.0
9.909910
2.033723
0.400000
C6
20
12.0
9.909910
2.033723
0.600000
E1
6
1.0
2.972973
1.196537
0.166667
E3
10
6.0
4.954955
1.515014
0.600000
E4
10
6.0
4.954955
1.515014
0.600000
E6
10
10.0
4.954955
1.515014
1.000000
E8
15
5.0
7.432432
1.808995
0.333333
Average
scores
were
used
for
ties.
Data
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Median
One­
Way
Analysis
Chi­
Square
18.3589
DF
7
Pr
>
Chi­
Square
0.0105
Data
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NONPARAMETRIC
COMPARISON
OF
FROG
WEIGHT
BETWEEN
PONDS
427
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
WEIGHT
Classified
by
Variable
POND
Sum
of
Expected
Std
Dev
Mean
POND
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C1
20
649.0
1020.0
117.344960
32.450000
C3
17
568.0
867.0
110.171950
33.411765
C6
20
1525.0
1020.0
117.344960
76.250000
E1
8
311.0
408.0
79.523118
38.875000
E3
10
681.0
510.0
87.948337
68.100000
E4
10
658.0
510.0
87.948337
65.800000
E6
10
484.0
510.0
87.948337
48.400000
E8
6
275.0
306.0
69.605629
45.833333
Average
scores
were
used
for
ties.

Kruskal­
Wallis
Test
Chi­
Square
36.5882
DF
7
Pr
>
Chi­
Square
<.
0001
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
WEIGHT
Classified
by
Variable
POND
Sum
of
Expected
Std
Dev
Mean
POND
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C1
20
4.0
9.900990
2.012363
0.200000
C3
17
4.0
8.415842
1.889352
0.235294
C6
20
18.0
9.900990
2.012363
0.900000
E1
8
2.0
3.960396
1.363751
0.250000
E3
10
6.0
4.950495
1.508236
0.600000
E4
10
9.0
4.950495
1.508236
0.900000
E6
10
4.0
4.950495
1.508236
0.400000
E8
6
3.0
2.970297
1.193675
0.500000
Average
scores
were
used
for
ties.
Data
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Median
One­
Way
Analysis
Chi­
Square
33.6219
DF
7
Pr
>
Chi­
Square
<.
0001
Data
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ANOVA
FOR
WEIGHT
OF
FROGS
BETWEEN
REFERENCE
(
REF)
AND
EXPERIMENTAL
(
EXP)
SITES
429
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
GROUP
2
EXP
REF
Number
of
observations
8
Dependent
Variable:
WEIGHT
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
75.510261
75.510261
0.44
0.5307
Error
6
1024.136445
170.689408
Corrected
Total
7
1099.646706
R­
Square
Coeff
Var
Root
MSE
WEIGHT
Mean
0.068668
32.30525
13.06482
40.44177
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
GROUP
1
75.51026075
75.51026075
0.44
0.5307
Levene's
Test
for
Homogeneity
of
WEIGHT
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
GROUP
1
64108.8
64108.8
1.83
0.2246
Error
6
209888
34981.3
Bartlett's
Test
for
Homogeneity
of
WEIGHT
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
GROUP
1
2.4716
0.1159
Dunnett's
t
Tests
for
WEIGHT
Data
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EPA
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Page
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NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
6
Error
Mean
Square
170.6894
Critical
Value
of
Dunnett's
t
2.44695
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
Simultaneous
GROUP
Between
95%
Confidence
Comparison
Means
Limits
REF
­
EXP
­
6.346
­
29.693
17.001
Data
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Page
50
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ANOVA
FOR
WEIGHT
OF
FROGS
BETWEEN
REFERENCE
(
REF)
AND
EXPERIMENTAL
(
EXP)
SITES
433
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
GROUP
2
EXP
REF
Number
of
observations
8
Dependent
Variable:
WEIGHT
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
2.6671491
2.6671491
0.06
0.8143
Error
6
265.7568743
44.2928124
Corrected
Total
7
268.4240234
R­
Square
Coeff
Var
Root
MSE
WEIGHT
Mean
0.009936
25.05318
6.655285
26.56463
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
GROUP
1
2.66714907
2.66714907
0.06
0.8143
Levene's
Test
for
Homogeneity
of
WEIGHT
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
GROUP
1
5070.2
5070.2
4.36
0.0818
Error
6
6973.5
1162.3
Bartlett's
Test
for
Homogeneity
of
WEIGHT
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
GROUP
1
1.8380
0.1752
Dunnett's
t
Tests
for
WEIGHT
Data
Evaluation
Report
on
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Hormone
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EPA
MRID
Number
458675­
01
Page
51
of
152
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
6
Error
Mean
Square
44.29281
Critical
Value
of
Dunnett's
t
2.44695
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
Simultaneous
GROUP
Between
95%
Confidence
Comparison
Means
Limits
REF
­
EXP
­
1.193
­
13.086
10.700
Data
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NONPARAMETRIC
COMPARISON
OF
FROG
WEIGHT
BETWEEN
GROUPS
437
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
WEIGHT
Classified
by
Variable
GROUP
Sum
of
Expected
Std
Dev
Mean
GROUP
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
EXP
5
23.0
22.50
3.354102
4.600000
REF
3
13.0
13.50
3.354102
4.333333
Wilcoxon
Two­
Sample
Test
Statistic
13.0000
Normal
Approximation
Z
0.0000
One­
Sided
Pr
<
Z
0.5000
Two­
Sided
Pr
>
|
Z|
1.0000
t
Approximation
One­
Sided
Pr
<
Z
0.5000
Two­
Sided
Pr
>
|
Z|
1.0000
Z
includes
a
continuity
correction
of
0.5.

Kruskal­
Wallis
Test
Chi­
Square
0.0222
DF
1
Pr
>
Chi­
Square
0.8815
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
WEIGHT
Classified
by
Variable
GROUP
Sum
of
Expected
Std
Dev
Mean
GROUP
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
EXP
5
3.0
2.50
0.731925
0.600000
REF
3
1.0
1.50
0.731925
0.333333
Data
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Median
Two­
Sample
Test
Statistic
1.0000
Z
­
0.6831
One­
Sided
Pr
<
Z
0.2473
Two­
Sided
Pr
>
|
Z|
0.4945
Median
One­
Way
Analysis
Chi­
Square
0.4667
DF
1
Pr
>
Chi­
Square
0.4945
Data
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NONPARAMETRIC
COMPARISON
OF
FROG
WEIGHT
BETWEEN
GROUPS
439
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
WEIGHT
Classified
by
Variable
GROUP
Sum
of
Expected
Std
Dev
Mean
GROUP
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
EXP
5
25.0
22.50
3.354102
5.000000
REF
3
11.0
13.50
3.354102
3.666667
Wilcoxon
Two­
Sample
Test
Statistic
11.0000
Normal
Approximation
Z
­
0.5963
One­
Sided
Pr
<
Z
0.2755
Two­
Sided
Pr
>
|
Z|
0.5510
t
Approximation
One­
Sided
Pr
<
Z
0.2849
Two­
Sided
Pr
>
|
Z|
0.5698
Z
includes
a
continuity
correction
of
0.5.

Kruskal­
Wallis
Test
Chi­
Square
0.5556
DF
1
Pr
>
Chi­
Square
0.4561
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
WEIGHT
Classified
by
Variable
GROUP
Sum
of
Expected
Std
Dev
Mean
GROUP
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
EXP
5
3.0
2.50
0.731925
0.600000
REF
3
1.0
1.50
0.731925
0.333333
Data
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Median
Two­
Sample
Test
Statistic
1.0000
Z
­
0.6831
One­
Sided
Pr
<
Z
0.2473
Two­
Sided
Pr
>
|
Z|
0.4945
Median
One­
Way
Analysis
Chi­
Square
0.4667
DF
1
Pr
>
Chi­
Square
0.4945
Data
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56
of
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MEAN
TESTICULAR
WEIGHTS
FOR
FROGS
COLLECTED
BY
POND
441
Obs
POND
GROUP
_
TYPE_
_
FREQ_
TESTES
T_
SD
T_
CV
1
C1
REF
0
20
0.024585
0.010550
42.9144
2
C3
REF
0
20
0.030953
0.023958
77.4001
3
C6
REF
0
20
0.047570
0.016667
35.0366
4
E1
EXP
0
10
0.036925
0.014242
38.5703
5
E3
EXP
0
10
0.053570
0.017665
32.9753
6
E4
EXP
0
10
0.057670
0.009618
16.6769
7
E6
EXP
0
10
0.033560
0.018074
53.8565
8
E8
EXP
0
10
0.044200
0.019885
44.9884
Data
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57
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ANOVA
FOR
TESTICULAR
WEIGHT
FOR
FROGS
BETWEEN
PONDS
442
The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
POND
8
C1
C3
C6
E1
E3
E4
E6
E8
Number
of
observations
8
Dependent
Variable:
TESTES
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
7
0.00093152
0.00013307
.
.

Error
0
0.00000000
.

Corrected
Total
7
0.00093152
R­
Square
Coeff
Var
Root
MSE
TESTES
Mean
1.000000
.
.
0.041129
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
POND
7
0.00093152
0.00013307
.
.

Levene's
Test
for
Homogeneity
of
TESTES
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
POND
0
0
.
.
.

Error
0
0
.

Bartlett's
Test
for
Homogeneity
of
TESTES
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
POND
0
0
.

ANOVA
FOR
TESTICULAR
WEIGHT
FOR
FROGS
BETWEEN
PONDS
445
The
ANOVA
Procedure
Level
of
­­­­­­­­­­­­
TESTES­­­­­­­­­­­
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POND
N
Mean
Std
Dev
C1
1
0.02458500
.

C3
1
0.03095294
.

C6
1
0.04757000
.

E1
1
0.03692500
.

E3
1
0.05357000
.

E4
1
0.05767000
.

E6
1
0.03356000
.

E8
1
0.04420000
.
Data
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Page
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of
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ANOVA
FOR
TESTICULAR
WEIGHT
FOR
FROGS
BETWEEN
REFERENCE
(
REF)
AND
EXPERIMENTAL
(
EXP)
SITE
446
The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
GROUP
2
EXP
REF
Number
of
observations
8
Dependent
Variable:
TESTES
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
0.00021934
0.00021934
1.85
0.2229
Error
6
0.00071218
0.00011870
Corrected
Total
7
0.00093152
R­
Square
Coeff
Var
Root
MSE
TESTES
Mean
0.235460
26.48934
0.010895
0.041129
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
GROUP
1
0.00021934
0.00021934
1.85
0.2229
Levene's
Test
for
Homogeneity
of
TESTES
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
GROUP
1
1.14E­
10
1.14E­
10
0.02
0.8818
Error
6
2.831E­
8
4.719E­
9
Bartlett's
Test
for
Homogeneity
of
TESTES
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
GROUP
1
0.0415
0.8386
Dunnett's
t
Tests
for
TESTES
Data
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Page
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NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
6
Error
Mean
Square
0.000119
Critical
Value
of
Dunnett's
t
2.44695
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
GROUP
Between
Simultaneous
95%

Comparison
Means
Confidence
Limits
REF
­
EXP
­
0.010816
­
0.030285
0.008653
Data
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Page
61
of
152
NONPARAMETRIC
COMPARISON
OF
FROG
TESTICULAR
WEIGHT
BETWEEN
TREATMENT
GROUPS
450
The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
TESTES
Classified
by
Variable
GROUP
Sum
of
Expected
Std
Dev
Mean
GROUP
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
EXP
5
27.0
22.50
3.354102
5.40
REF
3
9.0
13.50
3.354102
3.00
Wilcoxon
Two­
Sample
Test
Statistic
9.0000
Normal
Approximation
Z
­
1.1926
One­
Sided
Pr
<
Z
0.1165
Two­
Sided
Pr
>
|
Z|
0.2330
t
Approximation
One­
Sided
Pr
<
Z
0.1359
Two­
Sided
Pr
>
|
Z|
0.2719
Z
includes
a
continuity
correction
of
0.5.

Kruskal­
Wallis
Test
Chi­
Square
1.8000
DF
1
Pr
>
Chi­
Square
0.1797
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
TESTES
Classified
by
Variable
GROUP
Sum
of
Expected
Std
Dev
Mean
GROUP
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
EXP
5
3.0
2.50
0.731925
0.600000
REF
3
1.0
1.50
0.731925
0.333333
Median
Two­
Sample
Test
Data
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Statistic
1.0000
Z
­
0.6831
One­
Sided
Pr
<
Z
0.2473
Two­
Sided
Pr
>
|
Z|
0.4945
Median
One­
Way
Analysis
Chi­
Square
0.4667
DF
1
Pr
>
Chi­
Square
0.4945
Data
Evaluation
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Page
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MEAN
GONADOSOMATIC
INDEX
(
GSI)
OF
FROGS
COLLECTED
BY
POND
AND
SEX
452
Obs
POND
Sex
GROUP
_
TYPE_
_
FREQ_
GSI
GSI_
SD
GSI_
CV
1
C1
F
REF
0
20
1.20519
0.59529
49.3938
2
C1
M
REF
0
20
0.12568
0.02906
23.1225
3
C3
F
REF
0
20
2.32511
1.78135
76.6135
4
C3
M
REF
0
20
0.13554
0.04066
29.9998
5
C6
F
REF
0
20
1.56896
1.23447
78.6810
6
C6
M
REF
0
20
0.12992
0.03488
26.8432
7
E1
F
EXP
0
10
3.84820
3.58562
93.1766
8
E1
M
EXP
0
10
0.17529
0.04832
27.5681
9
E3
F
EXP
0
10
5.74161
2.38193
41.4853
10
E3
M
EXP
0
10
0.16518
0.03373
20.4210
11
E4
F
EXP
0
10
5.39806
1.45422
26.9397
12
E4
M
EXP
0
10
0.19433
0.03069
15.7908
13
E6
F
EXP
0
10
2.52463
1.94072
76.8714
14
E6
M
EXP
0
10
0.13001
0.04169
32.0636
15
E8
F
EXP
0
15
4.60581
3.38835
73.5669
16
E8
M
EXP
0
10
0.18583
0.02224
11.9674
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ANOVA
FOR
GSI
OF
FROGS
BETWEEN
PONDS
BY
SEX
453
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
POND
8
C1
C3
C6
E1
E3
E4
E6
E8
Number
of
observations
115
NOTE:
Due
to
missing
values,
only
111
observations
can
be
used
in
this
analysis.

Dependent
Variable:
GSI
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
7
292.3774342
41.7682049
10.12
<.
0001
Error
103
424.9868075
4.1260855
Corrected
Total
110
717.3642417
R­
Square
Coeff
Var
Root
MSE
GSI
Mean
0.407572
68.15863
2.031277
2.980220
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
POND
7
292.3774342
41.7682049
10.12
<.
0001
Levene's
Test
for
Homogeneity
of
GSI
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
POND
7
1421.7
203.1
7.65
<.
0001
Error
103
2732.9
26.5335
Bartlett's
Test
for
Homogeneity
of
GSI
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
Data
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POND
7
52.6276
<.
0001
Dunnett's
t
Tests
for
GSI
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
103
Error
Mean
Square
4.126086
Critical
Value
of
Dunnett's
t
2.69261
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
POND
Between
Simultaneous
95%

Comparison
Means
Confidence
Limits
E3
­
C1
4.5364
2.4181
6.6547
***

E4
­
C1
4.1929
2.0746
6.3112
***

E8
­
C1
3.4006
1.5325
5.2688
***

E1
­
C1
2.6430
0.0971
5.1889
***

E6
­
C1
1.3194
­
0.7989
3.4377
C3
­
C1
1.1199
­
0.6097
2.8495
C6
­
C1
0.3638
­
1.3658
2.0934
Data
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ANOVA
FOR
GSI
OF
FROGS
BETWEEN
PONDS
BY
SEX
457
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
POND
8
C1
C3
C6
E1
E3
E4
E6
E8
Number
of
observations
110
NOTE:
Due
to
missing
values,
only
101
observations
can
be
used
in
this
analysis.

Dependent
Variable:
GSI
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
7
0.06119627
0.00874232
6.84
<.
0001
Error
93
0.11878563
0.00127726
Corrected
Total
100
0.17998190
R­
Square
Coeff
Var
Root
MSE
GSI
Mean
0.340013
24.34181
0.035739
0.146821
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
POND
7
0.06119627
0.00874232
6.84
<.
0001
Levene's
Test
for
Homogeneity
of
GSI
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
POND
7
0.000018
2.513E­
6
0.86
0.5406
Error
93
0.000272
2.92E­
6
Bartlett's
Test
for
Homogeneity
of
GSI
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
Data
Evaluation
Report
on
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African
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EPA
MRID
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152
POND
7
5.9559
0.5449
Dunnett's
t
Tests
for
GSI
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
93
Error
Mean
Square
0.001277
Critical
Value
of
Dunnett's
t
2.70384
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
POND
Between
Simultaneous
95%

Comparison
Means
Confidence
Limits
E4
­
C1
0.06865
0.03122
0.10607
***

E8
­
C1
0.06015
0.01517
0.10513
***

E1
­
C1
0.04961
0.00918
0.09003
***

E3
­
C1
0.03950
0.00208
0.07693
***

C3
­
C1
0.00986
­
0.02202
0.04173
E6
­
C1
0.00433
­
0.03309
0.04176
C6
­
C1
0.00424
­
0.02632
0.03480
Data
Evaluation
Report
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and
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EPA
MRID
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Page
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NONPARAMETRIC
COMPARISON
OF
GSI
BETWEEN
PONDS
BY
SEX
461
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
GSI
Classified
by
Variable
POND
Sum
of
Expected
Std
Dev
Mean
POND
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C1
20
640.0
1120.0
130.332907
32.000000
C3
20
1005.0
1120.0
130.332907
50.250000
C6
20
780.0
1120.0
130.332907
39.000000
E1
6
347.0
336.0
76.681158
57.833333
E3
10
885.0
560.0
97.091023
88.500000
E4
10
887.0
560.0
97.091023
88.700000
E6
10
577.0
560.0
97.091023
57.700000
E8
15
1095.0
840.0
115.931014
73.000000
Kruskal­
Wallis
Test
Chi­
Square
42.0856
DF
7
Pr
>
Chi­
Square
<.
0001
The
NPAR1WAY
Procedure
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
GSI
Classified
by
Variable
POND
Sum
of
Expected
Std
Dev
Mean
POND
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C1
20
3.0
9.909910
2.033723
0.150000
C3
20
11.0
9.909910
2.033723
0.550000
C6
20
4.0
9.909910
2.033723
0.200000
E1
6
3.0
2.972973
1.196537
0.500000
E3
10
9.0
4.954955
1.515014
0.900000
E4
10
10.0
4.954955
1.515014
1.000000
E6
10
4.0
4.954955
1.515014
0.400000
E8
15
11.0
7.432432
1.808995
0.733333
Median
One­
Way
Analysis
Data
Evaluation
Report
on
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African
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and
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EPA
MRID
Number
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Page
69
of
152
Chi­
Square
36.9250
DF
7
Pr
>
Chi­
Square
<.
0001
Data
Evaluation
Report
on
South
African
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Support
 
Hormone
and
Aromatase
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EPA
MRID
Number
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01
Page
70
of
152
NONPARAMETRIC
COMPARISON
OF
GSI
BETWEEN
PONDS
BY
SEX
463
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
GSI
Classified
by
Variable
POND
Sum
of
Expected
Std
Dev
Mean
POND
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C1
20
714.0
1020.0
117.345643
35.700000
C3
17
783.0
867.0
110.172592
46.058824
C6
20
757.0
1020.0
117.345643
37.850000
E1
8
548.0
408.0
79.523581
68.500000
E3
10
645.0
510.0
87.948849
64.500000
E4
10
829.0
510.0
87.948849
82.900000
E6
10
394.0
510.0
87.948849
39.400000
E8
6
481.0
306.0
69.606034
80.166667
Kruskal­
Wallis
Test
Chi­
Square
34.3083
DF
7
Pr
>
Chi­
Square
<.
0001
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
GSI
Classified
by
Variable
POND
Sum
of
Expected
Std
Dev
Mean
POND
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C1
20
5.0
9.900990
2.012363
0.250000
C3
17
9.0
8.415842
1.889352
0.529412
C6
20
4.0
9.900990
2.012363
0.200000
E1
8
6.0
3.960396
1.363751
0.750000
E3
10
6.0
4.950495
1.508236
0.600000
E4
10
10.0
4.950495
1.508236
1.000000
E6
10
4.0
4.950495
1.508236
0.400000
E8
6
6.0
2.970297
1.193675
1.000000
Median
One­
Way
Analysis
Chi­
Square
30.7445
Data
Evaluation
Report
on
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EPA
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Number
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Page
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DF
7
Pr
>
Chi­
Square
<.
0001
Data
Evaluation
Report
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African
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and
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EPA
MRID
Number
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01
Page
72
of
152
ANOVA
FOR
GSI
OF
FROGS
BETWEEN
REFERENCE
(
REF)
AND
EXPERIMENTAL
(
EXP)
SITES
465
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
GROUP
2
EXP
REF
Number
of
observations
8
Dependent
Variable:
GSI
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
13.91190688
13.91190688
11.42
0.0149
Error
6
7.30985217
1.21830869
Corrected
Total
7
21.22175905
R­
Square
Coeff
Var
Root
MSE
GSI
Mean
0.655549
32.44289
1.103770
3.402195
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
GROUP
1
13.91190688
13.91190688
11.42
0.0149
Levene's
Test
for
Homogeneity
of
GSI
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
GROUP
1
2.3261
2.3261
1.69
0.2409
Error
6
8.2425
1.3737
Bartlett's
Test
for
Homogeneity
of
GSI
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
GROUP
1
1.1609
0.2813
Dunnett's
t
Tests
for
GSI
Data
Evaluation
Report
on
South
African
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Support
 
Hormone
and
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Analysis
EPA
MRID
Number
458675­
01
Page
73
of
152
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
6
Error
Mean
Square
1.218309
Critical
Value
of
Dunnett's
t
2.44695
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
GROUP
Between
Simultaneous
95%

Comparison
Means
Confidence
Limits
REF
­
EXP
­
2.7239
­
4.6963
­
0.7515
***
Data
Evaluation
Report
on
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African
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Hormone
and
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EPA
MRID
Number
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01
Page
74
of
152
ANOVA
FOR
GSI
OF
FROGS
BETWEEN
REFERENCE
(
REF)
AND
EXPERIMENTAL
(
EXP)
SITES
469
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
GROUP
2
EXP
REF
Number
of
observations
8
Dependent
Variable:
GSI
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
0.00296251
0.00296251
6.99
0.0383
Error
6
0.00254148
0.00042358
Corrected
Total
7
0.00550399
R­
Square
Coeff
Var
Root
MSE
GSI
Mean
0.538248
13.25886
0.020581
0.155225
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
GROUP
1
0.00296251
0.00296251
6.99
0.0383
Levene's
Test
for
Homogeneity
of
GSI
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
GROUP
1
4.36E­
7
4.36E­
7
1.49
0.2676
Error
6
1.753E­
6
2.921E­
7
Bartlett's
Test
for
Homogeneity
of
GSI
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
GROUP
1
3.4829
0.0620
Dunnett's
t
Tests
for
GSI
Data
Evaluation
Report
on
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African
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Hormone
and
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Analysis
EPA
MRID
Number
458675­
01
Page
75
of
152
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
6
Error
Mean
Square
0.000424
Critical
Value
of
Dunnett's
t
2.44695
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
GROUP
Between
Simultaneous
95%

Comparison
Means
Confidence
Limits
REF
­
EXP
­
0.03975
­
0.07653
­
0.00297
***
Data
Evaluation
Report
on
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African
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Hormone
and
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EPA
MRID
Number
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01
Page
76
of
152
NONPARAMETRIC
COMPARISON
OF
GSI
BETWEEN
TREATMENT
GROUPS
BY
SEX
473
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
GSI
Classified
by
Variable
GROUP
Sum
of
Expected
Std
Dev
Mean
GROUP
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
EXP
5
30.0
22.50
3.354102
6.0
REF
3
6.0
13.50
3.354102
2.0
Wilcoxon
Two­
Sample
Test
Statistic
6.0000
Normal
Approximation
Z
­
2.0870
One­
Sided
Pr
<
Z
0.0184
Two­
Sided
Pr
>
|
Z|
0.0369
t
Approximation
One­
Sided
Pr
<
Z
0.0377
Two­
Sided
Pr
>
|
Z|
0.0753
Z
includes
a
continuity
correction
of
0.5.

Kruskal­
Wallis
Test
Chi­
Square
5.0000
DF
1
Pr
>
Chi­
Square
0.0253
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
GSI
Classified
by
Variable
GROUP
Sum
of
Expected
Std
Dev
Mean
GROUP
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
EXP
5
4.0
2.50
0.731925
0.80
REF
3
0.0
1.50
0.731925
0.00
Data
Evaluation
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Median
Two­
Sample
Test
Statistic
0.0000
Z
­
2.0494
One­
Sided
Pr
<
Z
0.0202
Two­
Sided
Pr
>
|
Z|
0.0404
Median
One­
Way
Analysis
Chi­
Square
4.2000
DF
1
Pr
>
Chi­
Square
0.0404
Data
Evaluation
Report
on
South
African
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Support
 
Hormone
and
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Analysis
EPA
MRID
Number
458675­
01
Page
78
of
152
NONPARAMETRIC
COMPARISON
OF
GSI
BETWEEN
TREATMENT
GROUPS
BY
SEX
475
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
GSI
Classified
by
Variable
GROUP
Sum
of
Expected
Std
Dev
Mean
GROUP
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
EXP
5
29.0
22.50
3.354102
5.800000
REF
3
7.0
13.50
3.354102
2.333333
Wilcoxon
Two­
Sample
Test
Statistic
7.0000
Normal
Approximation
Z
­
1.7889
One­
Sided
Pr
<
Z
0.0368
Two­
Sided
Pr
>
|
Z|
0.0736
t
Approximation
One­
Sided
Pr
<
Z
0.0584
Two­
Sided
Pr
>
|
Z|
0.1168
Z
includes
a
continuity
correction
of
0.5.

Kruskal­
Wallis
Test
Chi­
Square
3.7556
DF
1
Pr
>
Chi­
Square
0.0526
The
NPAR1WAY
Procedure
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
GSI
Classified
by
Variable
GROUP
Sum
of
Expected
Std
Dev
Mean
GROUP
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
EXP
5
4.0
2.50
0.731925
0.80
Data
Evaluation
Report
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REF
3
0.0
1.50
0.731925
0.00
Median
Two­
Sample
Test
Statistic
0.0000
Z
­
2.0494
One­
Sided
Pr
<
Z
0.0202
Two­
Sided
Pr
>
|
Z|
0.0404
Median
One­
Way
Analysis
Chi­
Square
4.2000
DF
1
Pr
>
Chi­
Square
0.0404
Data
Evaluation
Report
on
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MEAN
CONDITION
INDEX
OF
FROGS
COLLECTED
BY
POND
AND
SEX
477
Obs
POND
Sex
GROUP
_
TYPE_
_
FREQ_
CONDITION
CI_
SD
CI_
CV
1
C1
F
REF
0
20
0.46055
0.10789
23.4271
2
C1
M
REF
0
20
0.32900
0.06735
20.4721
3
C3
F
REF
0
20
0.47447
0.19746
41.6174
4
C3
M
REF
0
20
0.33864
0.14453
42.6786
5
C6
F
REF
0
20
0.53024
0.09217
17.3826
6
C6
M
REF
0
20
0.50706
0.10983
21.6600
7
E1
F
EXP
0
10
0.41857
0.18672
44.6087
8
E1
M
EXP
0
10
0.37327
0.15799
42.3275
9
E3
F
EXP
0
10
0.52443
0.06631
12.6435
10
E3
M
EXP
0
10
0.48108
0.09040
18.7901
11
E4
F
EXP
0
10
0.61236
0.14012
22.8822
12
E4
M
EXP
0
10
0.46696
0.04620
9.8932
13
E6
F
EXP
0
10
0.74118
0.17227
23.2423
14
E6
M
EXP
0
10
0.40045
0.12809
31.9870
15
E8
F
EXP
0
15
0.42604
0.19041
44.6937
16
E8
M
EXP
0
10
0.37014
0.11131
30.0735
Data
Evaluation
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ANOVA
FOR
CONDITION
INDEX
OF
FROGS
BETWEEN
PONDS
BY
SEX
478
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
POND
8
C1
C3
C6
E1
E3
E4
E6
E8
Number
of
observations
115
NOTE:
Due
to
missing
values,
only
111
observations
can
be
used
in
this
analysis.

Dependent
Variable:
CI
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
7
0.87825562
0.12546509
5.65
<.
0001
Error
103
2.28870294
0.02222042
Corrected
Total
110
3.16695856
R­
Square
Coeff
Var
Root
MSE
CI
Mean
0.277318
29.03514
0.149065
0.513396
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
POND
7
0.87825562
0.12546509
5.65
<.
0001
Levene's
Test
for
Homogeneity
of
CI
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
POND
7
0.0167
0.00238
3.16
0.0046
Error
103
0.0775
0.000753
Bartlett's
Test
for
Homogeneity
of
CI
Variance
Data
Evaluation
Report
on
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African
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Source
DF
Chi­
Square
Pr
>
ChiSq
POND
7
23.0265
0.0017
Dunnett's
t
Tests
for
CI
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
103
Error
Mean
Square
0.02222
Critical
Value
of
Dunnett's
t
2.69261
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
POND
Between
Simultaneous
95%

Comparison
Means
Confidence
Limits
E6
­
C1
0.28063
0.12518
0.43608
***

E4
­
C1
0.15181
­
0.00365
0.30726
C6
­
C1
0.06969
­
0.05723
0.19662
E3
­
C1
0.06388
­
0.09157
0.21933
C3
­
C1
0.01392
­
0.11300
0.14085
E8
­
C1
­
0.03451
­
0.17161
0.10258
E1
­
C1
­
0.04198
­
0.22881
0.14485
Data
Evaluation
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ANOVA
FOR
CONDITION
INDEX
OF
FROGS
BETWEEN
PONDS
BY
SEX
482
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
POND
8
C1
C3
C6
E1
E3
E4
E6
E8
Number
of
observations
110
NOTE:
Due
to
missing
values,
only
101
observations
can
be
used
in
this
analysis.

Dependent
Variable:
CI
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
7
0.50980220
0.07282889
6.01
<.
0001
Error
93
1.12670273
0.01211508
Corrected
Total
100
1.63650493
R­
Square
Coeff
Var
Root
MSE
CI
Mean
0.311519
27.00248
0.110069
0.407624
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
POND
7
0.50980220
0.07282889
6.01
<.
0001
Levene's
Test
for
Homogeneity
of
CI
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
POND
7
0.00421
0.000602
1.65
0.1310
Error
93
0.0339
0.000365
Bartlett's
Test
for
Homogeneity
of
CI
Variance
Data
Evaluation
Report
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EPA
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Source
DF
Chi­
Square
Pr
>
ChiSq
POND
7
20.4868
0.0046
Dunnett's
t
Tests
for
CI
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
93
Error
Mean
Square
0.012115
Critical
Value
of
Dunnett's
t
2.70384
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
POND
Between
Simultaneous
95%

Comparison
Means
Confidence
Limits
C6
­
C1
0.17805
0.08394
0.27217
***

E3
­
C1
0.15208
0.03682
0.26734
***

E4
­
C1
0.13795
0.02269
0.25322
***

E6
­
C1
0.07145
­
0.04381
0.18672
E1
­
C1
0.04426
­
0.08024
0.16876
E8
­
C1
0.04114
­
0.09739
0.17967
C3
­
C1
0.00964
­
0.08853
0.10782
Data
Evaluation
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NONPARAMETRIC
COMPARISON
OF
CONDITION
INDEX
BETWEEN
PONDS
BY
SEX
486
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
CI
Classified
by
Variable
POND
Sum
of
Expected
Std
Dev
Mean
POND
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C1
20
901.0
1120.0
130.332907
45.050000
C3
20
982.0
1120.0
130.332907
49.100000
C6
20
1200.0
1120.0
130.332907
60.000000
E1
6
216.0
336.0
76.681158
36.000000
E3
10
607.0
560.0
97.091023
60.700000
E4
10
762.0
560.0
97.091023
76.200000
E6
10
946.0
560.0
97.091023
94.600000
E8
15
602.0
840.0
115.931014
40.133333
Kruskal­
Wallis
Test
Chi­
Square
28.0380
DF
7
Pr
>
Chi­
Square
0.0002
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
CI
Classified
by
Variable
POND
Sum
of
Expected
Std
Dev
Mean
POND
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C1
20
7.0
9.909910
2.033723
0.350000
C3
20
8.0
9.909910
2.033723
0.400000
C6
20
11.0
9.909910
2.033723
0.550000
E1
6
1.0
2.972973
1.196537
0.166667
E3
10
6.0
4.954955
1.515014
0.600000
E4
10
8.0
4.954955
1.515014
0.800000
E6
10
10.0
4.954955
1.515014
1.000000
E8
15
4.0
7.432432
1.808995
0.266667
Median
One­
Way
Analysis
Chi­
Square
22.5214
Data
Evaluation
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EPA
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DF
7
Pr
>
Chi­
Square
0.0021
Data
Evaluation
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EPA
MRID
Number
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Page
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NONPARAMETRIC
COMPARISON
OF
CONDITION
INDEX
BETWEEN
PONDS
BY
SEX
488
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
CI
Classified
by
Variable
POND
Sum
of
Expected
Std
Dev
Mean
POND
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C1
20
632.0
1020.0
117.345643
31.600000
C3
17
572.0
867.0
110.172592
33.647059
C6
20
1476.0
1020.0
117.345643
73.800000
E1
8
319.0
408.0
79.523581
39.875000
E3
10
701.0
510.0
87.948849
70.100000
E4
10
700.0
510.0
87.948849
70.000000
E6
10
480.0
510.0
87.948849
48.000000
E8
6
271.0
306.0
69.606034
45.166667
Kruskal­
Wallis
Test
Chi­
Square
36.7915
DF
7
Pr
>
Chi­
Square
<.
0001
The
NPAR1WAY
Procedure
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
CI
Classified
by
Variable
POND
Sum
of
Expected
Std
Dev
Mean
POND
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C1
20
2.0
9.900990
2.012363
0.100000
C3
17
4.0
8.415842
1.889352
0.235294
C6
20
17.0
9.900990
2.012363
0.850000
E1
8
3.0
3.960396
1.363751
0.375000
E3
10
7.0
4.950495
1.508236
0.700000
E4
10
10.0
4.950495
1.508236
1.000000
E6
10
3.0
4.950495
1.508236
0.300000
E8
6
4.0
2.970297
1.193675
0.666667
Median
One­
Way
Analysis
Data
Evaluation
Report
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Chi­
Square
41.3124
DF
7
Pr
>
Chi­
Square
<.
0001
Data
Evaluation
Report
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and
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MRID
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Page
89
of
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ANOVA
FOR
CONDITION
INDEX
OF
FROGS
BETWEEN
REFERENCE
(
REF)
AND
EXPERIMENTAL
(
EXP)
SITES
490
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
GROUP
2
EXP
REF
Number
of
observations
8
Dependent
Variable:
CONDITION
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
0.00589954
0.00589954
0.46
0.5212
Error
6
0.07630211
0.01271702
Corrected
Total
7
0.08220165
R­
Square
Coeff
Var
Root
MSE
CONDITION
Mean
0.071769
21.54234
0.112770
0.523480
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
GROUP
1
0.00589954
0.00589954
0.46
0.5212
Levene's
Test
for
Homogeneity
of
CONDITION
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
GROUP
1
0.000358
0.000358
2.43
0.1703
Error
6
0.000884
0.000147
Bartlett's
Test
for
Homogeneity
of
CONDITION
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
GROUP
1
2.5067
0.1134
Dunnett's
t
Tests
for
CONDITION
Data
Evaluation
Report
on
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Support
 
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and
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EPA
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Number
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Page
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of
152
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
6
Error
Mean
Square
0.012717
Critical
Value
of
Dunnett's
t
2.44695
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
GROUP
Between
Simultaneous
95%

Comparison
Means
Confidence
Limits
REF
­
EXP
­
0.05609
­
0.25761
0.14543
Data
Evaluation
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and
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MRID
Number
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Page
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of
152
ANOVA
FOR
CONDITION
INDEX
OF
FROGS
BETWEEN
REFERENCE
(
REF)
AND
EXPERIMENTAL
(
EXP)
SITES
494
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
GROUP
2
EXP
REF
Number
of
observations
8
Dependent
Variable:
CONDITION
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
0.00134800
0.00134800
0.26
0.6279
Error
6
0.03102745
0.00517124
Corrected
Total
7
0.03237545
R­
Square
Coeff
Var
Root
MSE
CONDITION
Mean
0.041636
17.61129
0.071911
0.408325
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
GROUP
1
0.00134800
0.00134800
0.26
0.6279
Levene's
Test
for
Homogeneity
of
CONDITION
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
GROUP
1
0.000038
0.000038
3.08
0.1298
Error
6
0.000074
0.000012
Bartlett's
Test
for
Homogeneity
of
CONDITION
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
GROUP
1
1.0139
0.3140
Data
Evaluation
Report
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and
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of
152
Dunnett's
t
Tests
for
CONDITION
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
6
Error
Mean
Square
0.005171
Critical
Value
of
Dunnett's
t
2.44695
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
GROUP
Between
Simultaneous
95%

Comparison
Means
Confidence
Limits
REF
­
EXP
­
0.02681
­
0.15532
0.10169
Data
Evaluation
Report
on
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African
Analytical
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Hormone
and
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Analysis
EPA
MRID
Number
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01
Page
93
of
152
NONPARAMETRIC
COMPARISON
OF
CONDITION
INDEX
BETWEEN
GROUPS
BY
SEX
498
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
CONDITION
Classified
by
Variable
GROUP
Sum
of
Expected
Std
Dev
Mean
GROUP
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
EXP
5
23.0
22.50
3.354102
4.600000
REF
3
13.0
13.50
3.354102
4.333333
Wilcoxon
Two­
Sample
Test
Statistic
13.0000
Normal
Approximation
Z
0.0000
One­
Sided
Pr
<
Z
0.5000
Two­
Sided
Pr
>
|
Z|
1.0000
t
Approximation
One­
Sided
Pr
<
Z
0.5000
Two­
Sided
Pr
>
|
Z|
1.0000
Z
includes
a
continuity
correction
of
0.5.

Kruskal­
Wallis
Test
Chi­
Square
0.0222
DF
1
Pr
>
Chi­
Square
0.8815
The
NPAR1WAY
Procedure
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
CONDITION
Classified
by
Variable
GROUP
Sum
of
Expected
Std
Dev
Mean
GROUP
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
EXP
5
3.0
2.50
0.731925
0.600000
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REF
3
1.0
1.50
0.731925
0.333333
Median
Two­
Sample
Test
Statistic
1.0000
Z
­
0.6831
One­
Sided
Pr
<
Z
0.2473
Two­
Sided
Pr
>
|
Z|
0.4945
Median
One­
Way
Analysis
Chi­
Square
0.4667
DF
1
Pr
>
Chi­
Square
0.4945
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NONPARAMETRIC
COMPARISON
OF
CONDITION
INDEX
BETWEEN
GROUPS
BY
SEX
500
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
CONDITION
Classified
by
Variable
GROUP
Sum
of
Expected
Std
Dev
Mean
GROUP
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
EXP
5
25.0
22.50
3.354102
5.000000
REF
3
11.0
13.50
3.354102
3.666667
Wilcoxon
Two­
Sample
Test
Statistic
11.0000
Normal
Approximation
Z
­
0.5963
One­
Sided
Pr
<
Z
0.2755
Two­
Sided
Pr
>
|
Z|
0.5510
t
Approximation
One­
Sided
Pr
<
Z
0.2849
Two­
Sided
Pr
>
|
Z|
0.5698
Z
includes
a
continuity
correction
of
0.5.

Kruskal­
Wallis
Test
Chi­
Square
0.5556
DF
1
Pr
>
Chi­
Square
0.4561
The
NPAR1WAY
Procedure
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
CONDITION
Classified
by
Variable
GROUP
Sum
of
Expected
Std
Dev
Mean
GROUP
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
EXP
5
3.0
2.50
0.731925
0.600000
Data
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REF
3
1.0
1.50
0.731925
0.333333
Median
Two­
Sample
Test
Statistic
1.0000
Z
­
0.6831
One­
Sided
Pr
<
Z
0.2473
Two­
Sided
Pr
>
|
Z|
0.4945
Median
One­
Way
Analysis
Chi­
Square
0.4667
DF
1
Pr
>
Chi­
Square
0.4945
Data
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MEAN
FEMALE
PLASMA
ESTRADIOL
CONCENTRATIONS
BY
SITE
AND
ANIMAL
NUMBER
82
Obs
Site
Ind_
No
_
TYPE_
_
FREQ_
E2
STD
CV
1
C1
21
0
3
4748.49
330.25
6.9549
2
C1
22
0
3
97.48
21.83
22.3947
3
C1
23
0
3
22.77
12.41
54.5215
4
C1
24
0
3
33025.69
7581.68
22.9569
5
C1
25
0
3
51.59
27.63
53.5657
6
C1
26
0
3
3137.18
267.56
8.5286
7
C1
27
0
3
3877.23
780.16
20.1215
8
C1
28
0
3
13.78
2.62
19.0307
9
C1
29
0
3
2781.17
285.40
10.2618
10
C1
30
0
3
110.61
25.45
23.0079
11
C1
31
0
3
25.21
10.25
40.6502
12
C1
32
0
3
5463.82
1002.76
18.3527
13
C1
33
0
3
49.35
24.61
49.8719
14
C1
34
0
3
292.85
184.51
63.0053
15
C1
35
0
3
55.43
25.69
46.3413
16
C1
36
0
3
6696.34
1406.15
20.9987
17
C1
37
0
3
.
.
.

18
C1
38
0
3
29043.30
0.00
0.0000
19
C1
39
0
3
16585.36
2876.22
17.3419
20
C1
40
0
3
14608.67
1333.87
9.1307
21
C3
21
0
3
18963.76
6968.94
36.7487
22
C3
22
0
3
69.64
8.83
12.6770
23
C3
23
0
3
242.59
62.08
25.5892
24
C3
24
0
3
7865.85
343.34
4.3649
25
C3
25
0
3
.
.
.

26
C3
26
0
3
367.45
87.33
23.7661
27
C3
27
0
3
6028.80
244.98
4.0635
28
C3
28
0
3
.
.
.

29
C3
29
0
3
145.50
58.21
40.0054
30
C3
30
0
3
6402.32
782.25
12.2182
31
C3
31
0
3
8629.88
1789.24
20.7330
32
C3
32
0
3
5017.56
551.41
10.9895
33
C3
33
0
3
33218.85
0.00
0.0000
34
C3
34
0
3
33.97
3.34
9.8194
35
C3
35
0
3
23.23
10.79
46.4362
36
C3
36
0
3
249.98
8.50
3.4014
37
C3
37
0
3
169.56
36.07
21.2708
38
C3
38
0
3
15342.62
4160.93
27.1200
39
C3
39
0
3
5834.65
1516.16
25.9854
40
C3
40
0
3
108.12
63.16
58.4186
41
C6
21
0
3
8718.85
770.64
8.8388
42
C6
22
0
3
112.90
30.66
27.1571
43
C6
23
0
3
.
.
.
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44
C6
24
0
3
4578.96
512.44
11.1912
45
C6
25
0
3
546.55
199.05
36.4200
46
C6
26
0
3
26.31
19.35
73.5299
47
C6
27
0
3
4376.85
163.55
3.7366
48
C6
28
0
3
31.89
5.44
17.0485
49
C6
29
0
3
5682.53
431.56
7.5946
50
C6
30
0
3
4741.45
502.20
10.5918
51
C6
31
0
3
73.75
21.62
29.3201
52
C6
32
0
3
3646.29
166.95
4.5785
53
C6
33
0
3
4181.47
.
.

54
C6
34
0
3
19.63
16.47
83.9291
55
C6
35
0
3
26.74
14.71
55.0063
56
C6
36
0
3
56754.90
0.00
0.0000
57
C6
37
0
3
4586.95
357.81
7.8006
58
C6
38
0
3
34923.37
1076.57
3.0827
59
C6
39
0
3
7233.54
943.02
13.0367
60
C6
40
0
3
105573.43
9858.48
9.3380
61
E1
1
0
3
260.32
44.45
17.0755
62
E1
2
0
3
1859.49
92.60
4.9797
63
E1
3
0
3
3229.19
374.05
11.5835
64
E1
4
0
3
680.29
79.79
11.7284
65
E1
5
0
3
46.21
19.26
41.6735
66
E1
6
0
3
.
.
.

67
E3
1
0
3
128.90
23.59
18.3008
68
E3
2
0
3
198.40
45.41
22.8901
69
E3
3
0
3
162.61
19.74
12.1382
70
E3
4
0
3
266.59
4.57
1.7142
71
E3
5
0
3
.
.
.
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MEAN
FEMALE
PLASMA
ESTRADIOL
CONCENTRATIONS
BY
SITE
AND
ANIMAL
NUMBER
83
Obs
Site
Ind_
No
_
TYPE_
_
FREQ_
E2
STD
CV
72
E3
6
0
3
206.28
25.87
12.5389
73
E3
7
0
3
89.89
11.12
12.3714
74
E3
8
0
3
296.97
13.75
4.6306
75
E3
9
0
3
367.43
44.06
11.9904
76
E3
10
0
3
122.72
13.99
11.3976
77
E4
1
0
3
1041.18
134.13
12.8821
78
E4
2
0
3
2967.96
495.96
16.7104
79
E4
3
0
3
1810.90
319.77
17.6580
80
E4
4
0
3
659.32
146.41
22.2065
81
E4
5
0
3
561.15
83.25
14.8360
82
E4
6
0
3
3533.16
209.97
5.9427
83
E4
7
0
3
781.20
25.54
3.2692
84
E4
8
0
3
76.74
4.14
5.3991
85
E4
9
0
3
7830.98
1283.47
16.3897
86
E4
10
0
3
2956.11
376.50
12.7363
87
E6
1
0
3
95.92
40.27
41.9873
88
E6
2
0
3
124.57
27.11
21.7657
89
E6
3
0
3
7.63
0.00
0.0000
90
E6
4
0
3
138.21
47.09
34.0689
91
E6
5
0
3
87.17
2.84
3.2539
92
E6
6
0
3
166.49
32.02
19.2329
93
E6
7
0
3
182.97
58.74
32.1016
94
E6
8
0
3
10.56
0.00
0.0000
95
E6
9
0
3
140.54
29.13
20.7254
96
E6
10
0
3
128.53
31.29
24.3410
97
E8
1
0
3
392.23
31.03
7.9118
98
E8
2
0
3
1894.28
290.91
15.3573
99
E8
3
0
3
248.29
16.21
6.5302
100
E8
4
0
3
739.52
140.60
19.0130
101
E8
5
0
3
3581.29
223.32
6.2359
102
E8
6
0
3
594.92
53.15
8.9345
103
E8
7
0
3
567.99
61.997
10.9152
104
E8
8
0
3
8292.57
562.256
6.7802
105
E8
9
0
3
253.48
13.662
5.3897
106
E8
10
0
3
245.23
35.042
14.2896
107
E8
11
0
3
490.67
159.633
32.5339
108
E8
12
0
3
114.38
23.707
20.7265
109
E8
13
0
3
.
.
.

110
E8
14
0
3
.
.
.

111
E8
15
0
3
.
.
.
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ANOVA
FOR
FEMALE
PLASMA
ESTRADIOL
BETWEEN
SITES
85
The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
Site
8
C1
C3
C6
E1
E3
E4
E6
E8
Number
of
observations
111
NOTE:
Due
to
missing
values,
only
102
observations
can
be
used
in
this
analysis.

Dependent
Variable:
E2
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
7
1995951818
285135974
1.69
0.1218
Error
94
15898863008
169136841
Corrected
Total
101
17894814826
R­
Square
Coeff
Var
Root
MSE
E2
Mean
0.111538
253.2194
13005.26
5135.965
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
Site
7
1995951818
285135974
1.69
0.1218
Levene's
Test
for
Homogeneity
of
E2
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
Site
7
6.25E18
8.929E17
1.19
0.3154
Error
94
7.045E19
7.495E17
Bartlett's
Test
for
Homogeneity
of
E2
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
Site
7
250.4
<.
0001
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Dunnett's
t
Tests
for
E2
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
94
Error
Mean
Square
1.6914E8
Critical
Value
of
Dunnett's
t
2.69946
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
Simultaneous
Site
Between
95%
Confidence
Comparison
Means
Limits
C6
­
C1
6587
­
4803
17977
C3
­
C1
­
312
­
11860
11235
E4
­
C1
­
4130
­
17846
9586
E8
­
C1
­
4901
­
17846
8045
E1
­
C1
­
5137
­
22783
12509
E3
­
C1
­
6147
­
20354
8059
E6
­
C1
­
6244
­
19959
7472
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NONPARAMETRIC
COMPARISON
OF
FEMALE
PLASMA
ESTRADIOL
BETWEEN
SITES
89
The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
E2
Classified
by
Variable
Site
Sum
of
Expected
Std
Dev
Mean
Site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C1
19
985.0
978.50
116.343958
51.842105
C3
18
1059.0
927.00
113.921025
58.833333
C6
19
1142.0
978.50
116.343958
60.105263
E1
5
247.0
257.50
64.520669
49.400000
E3
9
331.0
463.50
84.759955
36.777778
E4
10
603.0
515.00
88.863191
60.300000
E6
10
235.0
515.00
88.863191
23.500000
E8
12
651.0
618.00
96.280839
54.250000
Kruskal­
Wallis
Test
Chi­
Square
14.9116
DF
7
Pr
>
Chi­
Square
0.0371
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
E2
Classified
by
Variable
Site
Sum
of
Expected
Std
Dev
Mean
Site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C1
19
10.0
9.50
1.975719
0.526316
C3
18
9.0
9.00
1.934573
0.500000
C6
19
13.0
9.50
1.975719
0.684211
E1
5
3.0
2.50
1.095671
0.600000
E3
9
0.0
4.50
1.439369
0.000000
E4
10
9.0
5.00
1.509049
0.900000
E6
10
0.0
5.00
1.509049
0.000000
E8
12
7.0
6.00
1.635013
0.583333
Median
One­
Way
Analysis
Chi­
Square
28.2849
DF
7
Pr
>
Chi­
Square
0.0002
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FEMALE
PLASMA
ESTRADIOL
CONCENTRATIONS
BY
TREATMENT
91
Obs
TREAT
_
TYPE_
_
FREQ_
E2
STD
CV
1
ATRAZINE
0
153
1057.20
1810.05
171.211
2
CONTROL
0
180
8612.35
17414.02
202.198
ANOVA
FOR
FEMALE
PLASMA
ESTRADIOL
ACROSS
TREATMENTS
92
The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
TREAT
2
ATRAZINE
CONTROL
Number
of
observations
333
NOTE:
Due
to
missing
values,
only
302
observations
can
be
used
in
this
analysis.

Dependent
Variable:
E2
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
4277610072
4277610072
25.73
<.
0001
Error
300
49878301383
166261005
Corrected
Total
301
54155911454
R­
Square
Coeff
Var
Root
MSE
E2
Mean
0.078987
249.8882
12894.22
5159.997
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
TREAT
1
4277610072
4277610072
25.73
<.
0001
Levene's
Test
for
Homogeneity
of
E2
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
TREAT
1
6.662E18
6.662E18
7.08
0.0082
Error
300
2.821E20
9.404E17
Bartlett's
Test
for
Homogeneity
of
E2
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
TREAT
1
438.5
<.
0001
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Dunnett's
t
Tests
for
E2
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
300
Error
Mean
Square
1.6626E8
Critical
Value
of
Dunnett's
t
1.96790
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Simultaneous
Difference
95%

TREAT
Between
Confidence
Comparison
Means
Limits
CONTROL
­
ATRAZINE
7555
4624
10486
***
Data
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NONPARAMETRIC
COMPARISON
OF
FEMALE
PLASMA
ESTRADIOL
BETWEEN
TREATMENTS
96
The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
E2
Classified
by
Variable
TREAT
Sum
of
Expected
Std
Dev
Mean
TREAT
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
ATRAZINE
138
18405.0
20907.0
755.946352
133.369565
CONTROL
164
27348.0
24846.0
755.946352
166.756098
Average
scores
were
used
for
ties.

Wilcoxon
Two­
Sample
Test
Statistic
18405.0000
Normal
Approximation
Z
­
3.3091
One­
Sided
Pr
<
Z
0.0005
Two­
Sided
Pr
>
|
Z|
0.0009
t
Approximation
One­
Sided
Pr
<
Z
0.0005
Two­
Sided
Pr
>
|
Z|
0.0010
Z
includes
a
continuity
correction
of
0.5.

Kruskal­
Wallis
Test
Chi­
Square
10.9545
DF
1
Pr
>
Chi­
Square
0.0009
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
E2
Classified
by
Variable
TREAT
Sum
of
Expected
Std
Dev
Mean
TREAT
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
ATRAZINE
138
57.0
69.0
4.335590
0.413043
CONTROL
164
94.0
82.0
4.335590
0.573171
Data
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Average
scores
were
used
for
ties.

Median
Two­
Sample
Test
Statistic
57.0000
Z
­
2.7678
One­
Sided
Pr
<
Z
0.0028
Two­
Sided
Pr
>
|
Z|
0.0056
Median
One­
Way
Analysis
Chi­
Square
7.6607
DF
1
Pr
>
Chi­
Square
0.0056
Data
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MEAN
FEMALE
PLASMA
TESTOSTERONE
CONCENTRATIONS
BY
SITE
AND
ANIMAL
NUMBER
98
Obs
Site
Ind_
No
TREAT
_
TYPE_
_
FREQ_
T
STD
CV
1
C1
21
CONTROL
0
6
1934.52
262.42
13.565
2
C1
22
CONTROL
0
6
321.86
293.90
91.313
3
C1
23
CONTROL
0
6
86.37
34.90
40.408
4
C1
24
CONTROL
0
6
14971.64
3255.59
21.745
5
C1
25
CONTROL
0
6
139.76
121.89
87.217
6
C1
26
CONTROL
0
6
103.94
85.34
82.100
7
C1
27
CONTROL
0
6
7023.04
900.09
12.816
8
C1
28
CONTROL
0
6
120.39
143.49
119.182
9
C1
29
CONTROL
0
6
3169.52
186.57
5.887
10
C1
30
CONTROL
0
6
173.79
21.49
12.363
11
C1
31
CONTROL
0
6
21.74
4.23
19.473
12
C1
32
CONTROL
0
6
2863.16
374.89
13.094
13
C1
33
CONTROL
0
6
7710.56
11725.47
152.070
14
C1
34
CONTROL
0
6
882.00
742.98
84.238
15
C1
35
CONTROL
0
6
19.60
6.26
31.937
16
C1
36
CONTROL
0
6
2153.11
262.17
12.176
17
C1
37
CONTROL
0
6
.
.
.

18
C1
38
CONTROL
0
6
14521.65
0.00
0.000
19
C1
39
CONTROL
0
6
6802.20
479.18
7.045
20
C1
40
CONTROL
0
6
4473.92
192.11
4.294
21
C3
21
CONTROL
0
6
7195.61
2147.40
29.843
22
C3
22
CONTROL
0
6
182.50
51.98
28.479
23
C3
23
CONTROL
0
6
208.80
18.94
9.072
24
C3
24
CONTROL
0
6
2036.82
371.23
18.226
25
C3
25
CONTROL
0
6
.
.
.

26
C3
26
CONTROL
0
6
217.95
46.48
21.327
27
C3
27
CONTROL
0
6
2243.51
289.97
12.925
28
C3
28
CONTROL
0
6
177.61
172.35
97.040
29
C3
29
CONTROL
0
6
491.07
415.26
84.562
30
C3
30
CONTROL
0
6
2786.38
1293.81
46.433
31
C3
31
CONTROL
0
6
9437.32
1086.34
11.511
32
C3
32
CONTROL
0
6
2538.57
225.46
8.882
33
C3
33
CONTROL
0
6
13840.63
3033.06
21.914
34
C3
34
CONTROL
0
6
67.85
32.83
48.383
35
C3
35
CONTROL
0
6
71.99
48.21
66.959
36
C3
36
CONTROL
0
6
899.73
253.65
28.191
37
C3
37
CONTROL
0
6
198.41
0.00
0.000
38
C3
38
CONTROL
0
6
238.65
15.39
6.449
39
C3
39
CONTROL
0
6
6217.78
4372.15
70.317
40
C3
40
CONTROL
0
6
102.76
79.92
77.772
41
C6
21
CONTROL
0
6
1706.25
400.48
23.471
42
C6
22
CONTROL
0
6
278.77
15.39
5.521
43
C6
23
CONTROL
0
6
1442.72
311.10
21.564
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44
C6
24
CONTROL
0
6
627.55
65.07
10.369
45
C6
25
CONTROL
0
6
175.93
67.73
38.501
46
C6
26
CONTROL
0
6
36.73
16.11
43.849
47
C6
27
CONTROL
0
6
2129.90
518.64
24.351
48
C6
28
CONTROL
0
6
60.51
20.16
33.320
49
C6
29
CONTROL
0
6
1756.10
227.47
12.953
50
C6
30
CONTROL
0
6
1697.45
526.60
31.023
51
C6
31
CONTROL
0
6
14.63
3.13
21.411
52
C6
32
CONTROL
0
6
1888.82
361.66
19.147
53
C6
33
CONTROL
0
6
2307.73
238.14
10.319
54
C6
34
CONTROL
0
6
74.49
123.46
165.746
55
C6
35
CONTROL
0
6
45.12
8.36
18.519
56
C6
36
CONTROL
0
6
14815.70
5414.25
36.544
57
C6
37
CONTROL
0
6
1428.39
413.12
28.922
58
C6
38
CONTROL
0
6
3102.61
411.83
13.274
59
C6
39
CONTROL
0
6
3876.90
735.67
18.976
60
C6
40
CONTROL
0
6
3085.45
900.82
29.196
61
E1
1
ATRAZINE
0
6
34.64
4.44
12.830
62
E1
2
ATRAZINE
0
6
1532.07
142.84
9.323
63
E1
3
ATRAZINE
0
6
2127.42
184.66
8.680
64
E1
4
ATRAZINE
0
6
63.66
31.31
49.174
65
E1
5
ATRAZINE
0
6
44.52
33.72
75.747
66
E1
6
ATRAZINE
0
6
.
.
.

67
E3
1
ATRAZINE
0
6
119.73
13.69
11.430
68
E3
2
ATRAZINE
0
6
244.45
99.19
40.576
69
E3
3
ATRAZINE
0
6
154.14
42.81
27.772
70
E3
4
ATRAZINE
0
6
129.56
43.94
33.913
71
E3
5
ATRAZINE
0
6
6.00
0.00
0.000
Data
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Page
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MEAN
FEMALE
PLASMA
TESTOSTERONE
CONCENTRATIONS
BY
SITE
AND
ANIMAL
NUMBER
99
Obs
Site
Ind_
No
TREAT
_
TYPE_
_
FREQ_
T
STD
CV
72
E3
6
ATRAZINE
0
3
.
.
.

73
E3
7
ATRAZINE
0
6
63.25
42.27
66.832
74
E3
8
ATRAZINE
0
6
110.76
55.10
49.745
75
E3
9
ATRAZINE
0
6
136.19
118.46
86.983
76
E3
10
ATRAZINE
0
6
519.34
783.60
150.883
77
E4
1
ATRAZINE
0
6
501.40
214.98
42.876
78
E4
2
ATRAZINE
0
6
2196.90
244.47
11.128
79
E4
3
ATRAZINE
0
6
663.50
97.44
14.685
80
E4
4
ATRAZINE
0
6
396.55
37.25
9.392
81
E4
5
ATRAZINE
0
6
239.31
50.18
20.969
82
E4
6
ATRAZINE
0
6
4495.01
2233.98
49.699
83
E4
7
ATRAZINE
0
6
371.69
32.59
8.767
84
E4
8
ATRAZINE
0
6
103.61
44.09
42.550
85
E4
9
ATRAZINE
0
6
4473.15
870.89
19.469
86
E4
10
ATRAZINE
0
6
1838.00
170.56
9.280
87
E6
1
ATRAZINE
0
6
72.81
15.26
20.956
88
E6
2
ATRAZINE
0
6
37.52
10.78
28.742
89
E6
3
ATRAZINE
0
6
.
.
.

90
E6
4
ATRAZINE
0
6
99.46
19.78
19.888
91
E6
5
ATRAZINE
0
6
103.05
42.38
41.123
92
E6
6
ATRAZINE
0
6
154.92
30.48
19.675
93
E6
7
ATRAZINE
0
6
29.62
17.58
59.351
94
E6
8
ATRAZINE
0
6
.
.
.

95
E6
9
ATRAZINE
0
6
199.67
112.48
56.334
96
E6
10
ATRAZINE
0
6
70.86
10.91
15.391
97
E8
1
ATRAZINE
0
6
338.68
254.52
75.151
98
E8
2
ATRAZINE
0
6
2491.05
1026.81
41.220
99
E8
3
ATRAZINE
0
6
74.43
22.66
30.452
100
E8
4
ATRAZINE
0
6
186.29
26.58
14.270
101
E8
5
ATRAZINE
0
6
2214.21
1006.46
45.455
102
E8
6
ATRAZINE
0
6
110.45
18.60
16.842
103
E8
7
ATRAZINE
0
6
183.41
15.589
8.4997
104
E8
8
ATRAZINE
0
6
4381.40
601.186
13.7213
105
E8
9
ATRAZINE
0
6
74.89
28.512
38.0707
106
E8
10
ATRAZINE
0
6
34.50
15.136
43.8774
107
E8
11
ATRAZINE
0
6
109.70
24.201
22.0601
108
E8
12
ATRAZINE
0
6
348.47
37.360
10.7213
109
E8
13
ATRAZINE
0
5
.
.
.

110
E8
14
ATRAZINE
0
5
.
.
.

111
E8
15
ATRAZINE
0
5
.
.
.
Data
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EPA
MRID
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Page
111
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ANOVA
FOR
FEMALE
PLASMA
TESTOSTERONE
BETWEEN
SITES
101
The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
Site
8
C1
C3
C6
E1
E3
E4
E6
E8
Number
of
observations
111
NOTE:
Due
to
missing
values,
only
102
observations
can
be
used
in
this
analysis.

Dependent
Variable:
T
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
7
134465197
19209314
1.97
0.0673
Error
94
916631986
9751404
Corrected
Total
101
1051097183
R­
Square
Coeff
Var
Root
MSE
T
Mean
0.127928
168.4578
3122.724
1853.713
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
Site
7
134465196.7
19209313.8
1.97
0.0673
Levene's
Test
for
Homogeneity
of
T
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
Site
7
5.972E15
8.531E14
1.23
0.2963
Error
94
6.54E16
6.958E14
Bartlett's
Test
for
Homogeneity
of
T
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
Site
7
114.0
<.
0001
Dunnett's
t
Tests
for
T
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
Data
Evaluation
Report
on
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African
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Hormone
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Analysis
EPA
MRID
Number
458675­
01
Page
112
of
152
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
94
Error
Mean
Square
9751404
Critical
Value
of
Dunnett's
t
2.70019
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
Site
Between
Simultaneous
95%

Comparison
Means
Confidence
Limits
C3
­
C1
­
965.2
­
3700.9
1770.5
C6
­
C1
­
1524.7
­
4225.9
1176.6
E4
­
C1
­
2024.3
­
5318.5
1269.9
E8
­
C1
­
2673.3
­
5782.4
435.8
E1
­
C1
­
2791.8
­
7029.9
1446.3
E3
­
C1
­
3387.4
­
6799.4
24.6
E6
­
C1
­
3456.3
­
7010.0
97.5
Data
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EPA
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Page
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NONPARAMETRIC
COMPARISON
OF
FEMALE
PLASMA
TESTOSTERONE
ACROSS
SITES
105
The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
T
Classified
by
Variable
Site
Sum
of
Expected
Std
Dev
Mean
Site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C1
19
1173.0
978.50
116.343958
61.736842
C3
19
1145.0
978.50
116.343958
60.263158
C6
20
1122.0
1030.00
118.645129
56.100000
E1
5
171.0
257.50
64.520669
34.200000
E3
9
287.0
463.50
84.759955
31.888889
E4
10
634.0
515.00
88.863191
63.400000
E6
8
178.0
412.00
80.340940
22.250000
E8
12
543.0
618.00
96.280839
45.250000
Kruskal­
Wallis
Test
Chi­
Square
20.0577
DF
7
Pr
>
Chi­
Square
0.0054
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
T
Classified
by
Variable
Site
Sum
of
Expected
Std
Dev
Mean
Site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C1
19
12.0
9.50
1.975719
0.631579
C3
19
10.0
9.50
1.975719
0.526316
C6
20
13.0
10.00
2.014797
0.650000
E1
5
2.0
2.50
1.095671
0.400000
E3
9
1.0
4.50
1.439369
0.111111
E4
10
8.0
5.00
1.509049
0.800000
E6
8
0.0
4.00
1.364326
0.000000
E8
12
5.0
6.00
1.635013
0.416667
Median
One­
Way
Analysis
Chi­
Square
20.5428
DF
7
Pr
>
Chi­
Square
0.0045
Data
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152
MEAN
FEMALE
PLASMA
TESTOSTERONE
CONCENTRATIONS
BY
TREATMENT
107
Obs
TREAT
_
TYPE_
_
FREQ_
T
STD
CV
1
ATRAZINE
0
300
724.55
1270.60
175.364
2
CONTROL
0
360
2942.16
4636.87
157.601
ANOVA
FOR
FEMALE
PLASMA
TESTOSTERONE
ACROSS
TREATMENTS
108
The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
TREAT
2
ATRAZINE
CONTROL
Number
of
observations
111
NOTE:
Due
to
missing
values,
only
102
observations
can
be
used
in
this
analysis.

Dependent
Variable:
T
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
98659132
98659132
10.36
0.0017
Error
100
952438051
9524381
Corrected
Total
101
1051097183
R­
Square
Coeff
Var
Root
MSE
T
Mean
0.093863
166.4853
3086.160
1853.713
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
TREAT
1
98659131.80
98659131.80
10.36
0.0017
Levene's
Test
for
Homogeneity
of
T
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
TREAT
1
4.797E15
4.797E15
6.77
0.0107
Error
100
7.081E16
7.081E14
Bartlett's
Test
for
Homogeneity
of
T
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
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TREAT
1
50.9636
<.
0001
Dunnett's
t
Tests
for
T
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
100
Error
Mean
Square
9524381
Critical
Value
of
Dunnett's
t
1.98398
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
TREAT
Between
Simultaneous
95%

Comparison
Means
Confidence
Limits
CONTROL
­
ATRAZINE
1985.8
761.7
3209.9
***
Data
Evaluation
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MEAN
MALE
PLASMA
ESTRADIOL
CONCENTRATIONS
BY
SITE
AND
ANIMAL
NUMBER
112
Obs
Site
Ind_
No
TREAT
_
TYPE_
_
FREQ_
E2
STD
CV
1
C1
1
CONTROL
0
3
15.87
9.19
57.913
2
C1
2
CONTROL
0
3
4492.83
294.58
6.557
3
C1
3
CONTROL
0
3
2404.51
250.12
10.402
4
C1
4
CONTROL
0
3
8.01
2.40
29.951
5
C1
5
CONTROL
0
3
4130.31
228.71
5.537
6
C1
6
CONTROL
0
3
4077.53
326.52
8.008
7
C1
7
CONTROL
0
3
5541.93
1066.63
19.247
8
C1
8
CONTROL
0
3
12.86
4.63
35.962
9
C1
9
CONTROL
0
3
41.91
8.78
20.956
10
C1
10
CONTROL
0
3
31.25
11.07
35.417
11
C1
11
CONTROL
0
3
64380.80
0.00
0.000
12
C1
12
CONTROL
0
3
35.75
17.69
49.488
13
C1
13
CONTROL
0
3
.
.
.

14
C1
14
CONTROL
0
3
98.39
46.18
46.939
15
C1
15
CONTROL
0
3
24.46
3.23
13.187
16
C1
16
CONTROL
0
3
63399.39
0.00
0.000
17
C1
17
CONTROL
0
3
21.41
0.54
2.518
18
C1
18
CONTROL
0
3
13.60
7.82
57.470
19
C1
19
CONTROL
0
3
.
.
.

20
C1
20
CONTROL
0
3
55.10
19.47
35.334
21
C3
1
CONTROL
0
3
.
.
.

22
C3
2
CONTROL
0
3
4683.85
269.53
5.754
23
C3
3
CONTROL
0
3
6290.86
1152.91
18.327
24
C3
4
CONTROL
0
3
48.31
55.54
114.965
25
C3
5
CONTROL
0
3
40.76
9.89
24.262
26
C3
6
CONTROL
0
3
25.63
0.70
2.715
27
C3
7
CONTROL
0
3
23262.42
2510.43
10.792
28
C3
8
CONTROL
0
3
4743.00
257.49
5.429
29
C3
9
CONTROL
0
3
28.66
7.32
25.555
30
C3
10
CONTROL
0
3
6568.09
1080.88
16.456
31
C3
11
CONTROL
0
3
5361.80
893.98
16.673
32
C3
12
CONTROL
0
3
4603.38
113.50
2.466
33
C3
13
CONTROL
0
3
10.13
5.90
58.246
34
C3
14
CONTROL
0
3
62.73
23.13
36.871
35
C3
15
CONTROL
0
3
98.45
.
.

36
C3
16
CONTROL
0
3
315.76
84.04
26.617
37
C3
17
CONTROL
0
3
.
.
.

38
C6
1
CONTROL
0
3
.
.
.

39
C6
2
CONTROL
0
3
433.93
54.03
12.452
40
C6
3
CONTROL
0
3
4619.07
1398.84
30.284
41
C6
4
CONTROL
0
3
29462.37
0.00
0.000
42
C6
5
CONTROL
0
3
7268.35
.
.

43
C6
6
CONTROL
0
3
13.29
6.66
50.074
Data
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44
C6
7
CONTROL
0
3
4376.36
307.80
7.033
45
C6
8
CONTROL
0
3
7893.73
619.58
7.849
46
C6
9
CONTROL
0
3
18.34
7.19
39.233
47
C6
10
CONTROL
0
3
5383.19
1515.08
28.145
48
C6
11
CONTROL
0
3
16.80
9.09
54.124
49
C6
12
CONTROL
0
3
26817.92
834.57
3.112
50
C6
13
CONTROL
0
3
3.37
0.00
0.000
51
C6
14
CONTROL
0
3
12815.92
2261.06
17.643
52
C6
15
CONTROL
0
3
10.54
0.00
0.000
53
C6
16
CONTROL
0
3
6065.52
199.53
3.290
54
C6
17
CONTROL
0
3
9.45
0.00
0.000
55
C6
18
CONTROL
0
3
4763.25
1793.75
37.658
56
C6
19
CONTROL
0
3
82865.65
.
.

57
C6
20
CONTROL
0
3
5527.13
292.49
5.292
58
E1
1
ATRAZINE
0
3
193.25
37.43
19.368
59
E1
2
ATRAZINE
0
3
238.75
12.20
5.109
60
E1
3
ATRAZINE
0
3
6.11
0.00
0.000
61
E1
4
ATRAZINE
0
3
106.33
44.92
42.243
62
E1
5
ATRAZINE
0
3
187.66
47.96
25.559
63
E1
6
ATRAZINE
0
3
6.85
0.00
0.000
64
E1
7
ATRAZINE
0
3
8.03
0.00
0.000
65
E1
8
ATRAZINE
0
3
9991.09
2055.96
20.578
66
E3
1
ATRAZINE
0
3
201.97
70.70
35.004
67
E3
2
ATRAZINE
0
3
182.84
7.29
3.987
68
E3
3
ATRAZINE
0
3
507.43
53.75
10.592
69
E3
4
ATRAZINE
0
3
9399.57
1177.61
12.528
70
E3
5
ATRAZINE
0
3
244.15
39.68
16.254
71
E3
6
ATRAZINE
0
3
263.68
31.09
11.790
Data
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MEAN
MALE
PLASMA
ESTRADIOL
CONCENTRATIONS
BY
SITE
AND
ANIMAL
NUMBER
113
Obs
Site
Ind_
No
TREAT
_
TYPE_
_
FREQ_
E2
STD
CV
72
E3
7
ATRAZINE
0
3
7777.23
759.22
9.762
73
E3
8
ATRAZINE
0
3
182.68
27.28
14.934
74
E3
9
ATRAZINE
0
3
5.14
0.00
0.000
75
E3
10
ATRAZINE
0
3
149.09
13.68
9.179
76
E4
1
ATRAZINE
0
3
7943.10
3964.57
49.912
77
E4
2
ATRAZINE
0
3
150.47
18.66
12.403
78
E4
3
ATRAZINE
0
3
79.94
52.95
66.242
79
E4
4
ATRAZINE
0
3
36.94
21.52
58.246
80
E4
5
ATRAZINE
0
3
3410.68
436.81
12.807
81
E4
6
ATRAZINE
0
3
3640.90
352.58
9.684
82
E4
7
ATRAZINE
0
3
.
.
.

83
E4
8
ATRAZINE
0
3
6524.77
286.56
4.392
84
E4
9
ATRAZINE
0
3
20742.65
2690.87
12.973
85
E4
10
ATRAZINE
0
3
23.56
24.36
103.364
86
E6
1
ATRAZINE
0
3
108.45
39.44
36.370
87
E6
2
ATRAZINE
0
3
29.56
18.42
62.328
88
E6
3
ATRAZINE
0
3
103.20
11.59
11.230
89
E6
4
ATRAZINE
0
3
129.57
48.82
37.675
90
E6
5
ATRAZINE
0
3
166.00
15.83
9.538
91
E6
6
ATRAZINE
0
3
63.51
12.19
19.192
92
E6
7
ATRAZINE
0
3
180.53
104.00
57.608
93
E6
8
ATRAZINE
0
3
6032.94
293.69
4.868
94
E6
9
ATRAZINE
0
3
131.07
19.68
15.011
95
E6
10
ATRAZINE
0
3
.
.
.

96
E8
1
ATRAZINE
0
3
86.70
21.36
24.632
97
E8
2
ATRAZINE
0
3
192.36
28.20
14.662
98
E8
3
ATRAZINE
0
3
2861.36
166.52
5.820
99
E8
4
ATRAZINE
0
3
.
.
.

100
E8
5
ATRAZINE
0
3
134.59
26.26
19.513
101
E8
6
ATRAZINE
0
3
129.23
58.11
44.970
Data
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ANOVA
FOR
MALE
PLASMA
ESTRADIOL
BETWEEN
SITES
114
The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
Site
8
C1
C3
C6
E1
E3
E4
E6
E8
Number
of
observations
101
NOTE:
Due
to
missing
values,
only
93
observations
can
be
used
in
this
analysis.

Dependent
Variable:
E2
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
7
1231861396
175980199
1.00
0.4368
Error
85
14951054355
175894757
Corrected
Total
92
16182915750
R­
Square
Coeff
Var
Root
MSE
E2
Mean
0.076121
253.8687
13262.53
5224.170
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
Site
7
1231861396
175980199
1.00
0.4368
Levene's
Test
for
Homogeneity
of
E2
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
Site
7
2.821E18
4.031E17
0.82
0.5770
Error
85
4.201E19
4.943E17
Bartlett's
Test
for
Homogeneity
of
E2
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
Site
7
92.5314
<.
0001
Dunnett's
t
Tests
for
E2
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
Data
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EPA
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against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
85
Error
Mean
Square
1.7589E8
Critical
Value
of
Dunnett's
t
2.70761
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Simultaneous
Difference
95%

Site
Between
Confidence
Comparison
Means
Limits
C6
­
C1
2174
­
9637
13986
E4
­
C1
­
3538
­
18198
11122
C3
­
C1
­
4523
­
17077
8031
E3
­
C1
­
6375
­
20537
7788
E1
­
C1
­
6924
­
22182
8335
E6
­
C1
­
7494
­
22154
7166
E8
­
C1
­
7585
­
25738
10568
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NONPARAMETRIC
COMPARISON
OF
MALE
PLASMA
ESTRADIOL
BETWEEN
SITES
118
The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
E2
Classified
by
Variable
Site
Sum
of
Expected
Std
Dev
Mean
Site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C1
18
711.0
846.0
102.834819
39.500000
C3
15
735.0
705.0
95.734006
49.000000
C6
19
1055.0
893.0
104.946018
55.526316
E1
8
284.0
376.0
72.984017
35.500000
E3
10
517.0
470.0
80.632913
51.700000
E4
9
484.0
423.0
76.954532
53.777778
E6
9
366.0
423.0
76.954532
40.666667
E8
5
219.0
235.0
58.708319
43.800000
Kruskal­
Wallis
Test
Chi­
Square
6.2571
DF
7
Pr
>
Chi­
Square
0.5101
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
E2
Classified
by
Variable
Site
Sum
of
Expected
Std
Dev
Mean
Site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C1
18
7.0
8.903226
1.915216
0.388889
C3
15
8.0
7.419355
1.782969
0.533333
C6
19
13.0
9.397849
1.954536
0.684211
E1
8
4.0
3.956989
1.359269
0.500000
E3
10
6.0
4.946237
1.501724
0.600000
E4
9
5.0
4.451613
1.433217
0.555556
E6
9
1.0
4.451613
1.433217
0.111111
E8
5
2.0
2.473118
1.093396
0.400000
Median
One­
Way
Analysis
Chi­
Square
9.5763
DF
7
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Pr
>
Chi­
Square
0.2139
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MEAN
MALE
PLASMA
ESTRADIOL
CONCENTRATIONS
BY
TREATMENT
120
Obs
TREAT
_
TYPE_
_
FREQ_
E2
STD
CV
1
ATRAZINE
0
132
2013.51
4174.05
207.302
2
CONTROL
0
171
6878.78
15084.70
219.293
ANOVA
FOR
MALE
PLASMA
ESTRADIOL
ACROSS
TREATMENTS
121
The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
TREAT
2
ATRAZINE
CONTROL
Number
of
observations
101
NOTE:
Due
to
missing
values,
only
93
observations
can
be
used
in
this
analysis.

ANOVA
FOR
MALE
PLASMA
ESTRADIOL
ACROSS
TREATMENTS
122
The
ANOVA
Procedure
Dependent
Variable:
E2
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
755878968
755878968
4.46
0.0375
Error
91
15427036782
169527877
Corrected
Total
92
16182915750
R­
Square
Coeff
Var
Root
MSE
E2
Mean
0.046708
249.2317
13020.29
5224.170
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
TREAT
1
755878968.2
755878968.2
4.46
0.0375
Levene's
Test
for
Homogeneity
of
E2
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
TREAT
1
1.63E18
1.63E18
3.08
0.0826
Error
91
4.814E19
5.291E17
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Bartlett's
Test
for
Homogeneity
of
E2
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
TREAT
1
63.5686
<.
0001
Dunnett's
t
Tests
for
E2
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
91
Error
Mean
Square
1.6953E8
Critical
Value
of
Dunnett's
t
1.98639
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Simultaneous
Difference
95%

TREAT
Between
Confidence
Comparison
Means
Limits
CONTROL
­
ATRAZINE
5742
340
11144
***
Data
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MEAN
MALE
PLASMA
TESTOSTERONE
CONCENTRATIONS
BY
SITE
AND
ANIMAL
NUMBER
125
Obs
Site
Ind_
No
TREAT
_
TYPE_
_
FREQ_
T
STD
CV
1
C1
1
CONTROL
0
6
139.03
139.95
100.663
2
C1
2
CONTROL
0
6
2737.00
242.96
8.877
3
C1
3
CONTROL
0
6
1674.80
769.50
45.946
4
C1
4
CONTROL
0
6
83.59
28.35
33.911
5
C1
5
CONTROL
0
6
2696.11
388.14
14.396
6
C1
6
CONTROL
0
6
2581.18
188.89
7.318
7
C1
7
CONTROL
0
6
2896.29
226.63
7.825
8
C1
8
CONTROL
0
6
194.78
39.95
20.511
9
C1
9
CONTROL
0
6
144.59
27.16
18.786
10
C1
10
CONTROL
0
6
82.04
6.21
7.566
11
C1
11
CONTROL
0
6
32190.40
0.00
0.000
12
C1
12
CONTROL
0
6
113.76
24.18
21.255
13
C1
13
CONTROL
0
6
.
.
.

14
C1
14
CONTROL
0
6
59.60
13.25
22.232
15
C1
15
CONTROL
0
6
57.70
18.65
32.317
16
C1
16
CONTROL
0
6
21112.00
0.00
0.000
17
C1
17
CONTROL
0
6
31.36
5.77
18.406
18
C1
18
CONTROL
0
6
88.85
15.25
17.161
19
C1
19
CONTROL
0
6
68.54
4.30
6.279
20
C1
20
CONTROL
0
6
161.21
35.78
22.196
21
C3
1
CONTROL
0
6
.
.
.

22
C3
2
CONTROL
0
6
2208.90
518.72
23.483
23
C3
3
CONTROL
0
6
9935.35
1378.10
13.871
24
C3
4
CONTROL
0
6
592.11
68.26
11.528
25
C3
5
CONTROL
0
6
265.97
175.53
65.994
26
C3
6
CONTROL
0
6
654.53
239.70
36.622
27
C3
7
CONTROL
0
6
8145.75
547.31
6.719
28
C3
8
CONTROL
0
6
2755.58
1362.81
49.456
29
C3
9
CONTROL
0
6
.
.
.

30
C3
10
CONTROL
0
6
2611.80
296.82
11.364
31
C3
11
CONTROL
0
6
2376.55
142.75
6.006
32
C3
12
CONTROL
0
6
2372.41
421.51
17.767
33
C3
13
CONTROL
0
6
159.89
97.48
60.967
34
C3
14
CONTROL
0
6
3556.26
417.34
11.735
35
C3
15
CONTROL
0
6
221.63
28.98
13.076
36
C3
16
CONTROL
0
6
190.61
137.99
72.396
37
C3
17
CONTROL
0
6
.
.
.

38
C6
1
CONTROL
0
6
79.25
22.98
28.991
39
C6
2
CONTROL
0
6
1217.66
856.84
70.368
40
C6
3
CONTROL
0
6
3018.72
506.00
16.762
41
C6
4
CONTROL
0
6
12570.29
909.41
7.235
42
C6
5
CONTROL
0
6
3905.44
1376.31
35.241
43
C6
6
CONTROL
0
6
224.60
40.01
17.816
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44
C6
7
CONTROL
0
6
1857.56
339.98
18.302
45
C6
8
CONTROL
0
6
4493.02
1951.16
43.426
46
C6
9
CONTROL
0
6
685.27
98.23
14.334
47
C6
10
CONTROL
0
6
2264.66
220.37
9.731
48
C6
11
CONTROL
0
6
602.58
86.20
14.305
49
C6
12
CONTROL
0
6
1928.61
329.99
17.110
50
C6
13
CONTROL
0
6
204.25
37.57
18.393
51
C6
14
CONTROL
0
6
5314.58
1069.39
20.122
52
C6
15
CONTROL
0
6
1034.23
35.23
3.4068
53
C6
16
CONTROL
0
6
3573.74
1077.58
30.1527
54
C6
17
CONTROL
0
6
9867.56
773.75
7.8413
55
C6
18
CONTROL
0
6
1494.39
143.13
9.5777
56
C6
19
CONTROL
0
6
7387.18
1162.12
15.7315
57
C6
20
CONTROL
0
6
1603.54
282.26
17.6021
58
E1
1
ATRAZINE
0
6
831.37
85.38
10.2698
59
E1
2
ATRAZINE
0
6
484.80
16.27
3.3550
60
E1
3
ATRAZINE
0
6
763.44
23.01
3.0134
61
E1
4
ATRAZINE
0
6
245.16
33.41
13.6296
62
E1
5
ATRAZINE
0
6
192.02
56.66
29.5075
63
E1
6
ATRAZINE
0
6
146.08
12.43
8.5100
64
E1
7
ATRAZINE
0
6
56.13
6.37
11.3552
65
E1
8
ATRAZINE
0
6
8262.43
1120.15
13.5572
66
E3
1
ATRAZINE
0
6
598.56
46.73
7.8074
67
E3
2
ATRAZINE
0
6
324.72
35.74
11.0078
68
E3
3
ATRAZINE
0
6
595.42
198.93
33.4092
69
E3
4
ATRAZINE
0
6
5124.19
260.81
5.0898
70
E3
5
ATRAZINE
0
6
440.39
59.79
13.5763
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MEAN
MALE
PLASMA
TESTOSTERONE
CONCENTRATIONS
BY
SITE
AND
ANIMAL
NUMBER
126
Obs
Site
Ind_
No
TREAT
_
TYPE_
_
FREQ_
T
STD
CV
71
E3
6
ATRAZINE
0
6
255.93
28.83
11.2640
72
E3
7
ATRAZINE
0
6
3936.60
660.75
16.7849
73
E3
8
ATRAZINE
0
6
138.52
48.12
34.7396
74
E3
9
ATRAZINE
0
6
250.10
32.27
12.9048
75
E3
10
ATRAZINE
0
6
252.42
39.72
15.7351
76
E4
1
ATRAZINE
0
6
5144.47
203.92
3.9639
77
E4
2
ATRAZINE
0
6
1060.29
112.53
10.6135
78
E4
3
ATRAZINE
0
6
2872.23
1444.02
50.2754
79
E4
4
ATRAZINE
0
6
1546.88
560.44
36.2305
80
E4
5
ATRAZINE
0
6
1638.72
291.68
17.7991
81
E4
6
ATRAZINE
0
6
3915.86
898.21
22.9377
82
E4
7
ATRAZINE
0
6
.
.
.

83
E4
8
ATRAZINE
0
6
5112.10
796.32
15.5771
84
E4
9
ATRAZINE
0
6
15218.92
2038.78
13.3963
85
E4
10
ATRAZINE
0
6
4992.90
3455.19
69.2020
86
E6
1
ATRAZINE
0
6
185.87
12.22
6.5729
87
E6
2
ATRAZINE
0
6
86.22
22.24
25.7912
88
E6
3
ATRAZINE
0
6
685.96
218.27
31.8191
89
E6
4
ATRAZINE
0
6
124.22
6.12
4.9292
90
E6
5
ATRAZINE
0
6
179.40
42.24
23.5432
91
E6
6
ATRAZINE
0
6
63.85
8.17
12.7968
92
E6
7
ATRAZINE
0
6
92.07
3.03
3.2897
93
E6
8
ATRAZINE
0
6
2759.97
218.01
7.8989
94
E6
9
ATRAZINE
0
6
47.38
18.61
39.2792
95
E6
10
ATRAZINE
0
6
.
.
.

96
E8
1
ATRAZINE
0
6
241.58
54.17
22.4250
97
E8
2
ATRAZINE
0
6
503.73
56.20
11.1575
98
E8
3
ATRAZINE
0
6
1441.63
307.45
21.3265
99
E8
4
ATRAZINE
0
6
.
.
.

100
E8
5
ATRAZINE
0
6
876.52
152.11
17.3543
101
E8
6
ATRAZINE
0
6
81.07
20.93
25.8156
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ANOVA
FOR
MALE
PLASMA
TESTOSTERONE
BETWEEN
SITES
127
The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
Site
8
C1
C3
C6
E1
E3
E4
E6
E8
Number
of
observations
101
NOTE:
Due
to
missing
values,
only
94
observations
can
be
used
in
this
analysis.

Dependent
Variable:
T
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
7
151109859
21587123
1.00
0.4339
Error
86
1848208528
21490797
Corrected
Total
93
1999318387
R­
Square
Coeff
Var
Root
MSE
T
Mean
0.075581
182.8974
4635.817
2534.654
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
Site
7
151109858.7
21587122.7
1.00
0.4339
Levene's
Test
for
Homogeneity
of
T
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
Site
7
5.575E16
7.964E15
0.96
0.4620
Error
86
7.098E17
8.254E15
Bartlett's
Test
for
Homogeneity
of
T
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
Site
7
68.1321
<.
0001
Dunnett's
t
Tests
for
T
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.
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Alpha
0.05
Error
Degrees
of
Freedom
86
Error
Mean
Square
21490797
Critical
Value
of
Dunnett's
t
2.70970
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Simultaneous
Difference
95%

Site
Between
Confidence
Comparison
Means
Limits
E4
­
C1
1079
­
4004
6162
C6
­
C1
­
366
­
4390
3658
C3
­
C1
­
957
­
5382
3467
E1
­
C1
­
2160
­
7454
3135
E3
­
C1
­
2341
­
7248
2567
E8
­
C1
­
2903
­
9217
3410
E6
­
C1
­
3063
­
8146
2020
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NONPARAMETRIC
COMPARISON
OF
MALE
PLASMA
TESTOSTERONE
ACROSS
SITES
131
The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
T
Classified
by
Variable
Site
Sum
of
Expected
Std
Dev
Mean
Site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C1
19
674.0
902.50
106.213229
35.473684
C3
14
777.0
665.00
94.162979
55.500000
C6
20
1214.0
950.00
108.243553
60.700000
E1
8
305.0
380.00
73.801536
38.125000
E3
10
441.0
475.00
81.547532
44.100000
E4
9
655.0
427.50
77.821912
72.777778
E6
9
215.0
427.50
77.821912
23.888889
E8
5
184.0
237.50
59.354163
36.800000
Kruskal­
Wallis
Test
Chi­
Square
25.9190
DF
7
Pr
>
Chi­
Square
0.0005
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
T
Classified
by
Variable
Site
Sum
of
Expected
Std
Dev
Mean
Site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C1
19
7.0
9.50
1.957203
0.368421
C3
14
8.0
7.00
1.735152
0.571429
C6
20
15.0
10.00
1.994616
0.750000
E1
8
3.0
4.00
1.359949
0.375000
E3
10
2.0
5.00
1.502686
0.200000
E4
9
9.0
4.50
1.434033
1.000000
E6
9
1.0
4.50
1.434033
0.111111
E8
5
2.0
2.50
1.093726
0.400000
Median
One­
Way
Analysis
Chi­
Square
25.0763
DF
7
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Pr
>
Chi­
Square
0.0007
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MEAN
MALE
PLASMA
TESTOSTERONE
CONCENTRATIONS
BY
TREATMENT
133
Obs
TREAT
_
TYPE_
_
FREQ_
T
STD
CV
1
ATRAZINE
0
264
1750.49
2943.22
168.137
2
CONTROL
0
342
3113.39
5255.50
168.803
ANOVA
FOR
MALE
PLASMA
TESTOSTERONE
ACROSS
TREATMENTS
134
The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
TREAT
2
ATRAZINE
CONTROL
Number
of
observations
101
NOTE:
Due
to
missing
values,
only
94
observations
can
be
used
in
this
analysis.

Dependent
Variable:
T
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
44714480
44714480
2.10
0.1503
Error
92
1954603906
21245695
Corrected
Total
93
1999318387
R­
Square
Coeff
Var
Root
MSE
T
Mean
0.022365
181.8515
4609.305
2534.654
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
TREAT
1
44714480.50
44714480.50
2.10
0.1503
Levene's
Test
for
Homogeneity
of
T
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
TREAT
1
1.147E16
1.147E16
1.30
0.2579
Error
92
8.145E17
8.853E15
Data
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Bartlett's
Test
for
Homogeneity
of
T
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
TREAT
1
16.9361
<.
0001
Dunnett's
t
Tests
for
T
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
92
Error
Mean
Square
21245695
Critical
Value
of
Dunnett's
t
1.98609
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
TREAT
Between
Simultaneous
95%

Comparison
Means
Confidence
Limits
CONTROL
­
ATRAZINE
1390.8
­
513.2
3294.8
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MEAN
FEMALE
AROMATASE
ACTIVITY
BY
SITE
AND
ANIMAL
NUMBER
138
Obs
Site
Ind_
No
TREAT
_
TYPE_
_
FREQ_
A
STD
CV
1
C1
21
CONTROL
0
2
153.70
5.5665
3.6218
2
C1
22
CONTROL
0
2
26.59
1.0643
4.0021
3
C1
23
CONTROL
0
2
80.68
7.1499
8.8622
4
C1
24
CONTROL
0
2
160.21
13.3182
8.3131
5
C1
25
CONTROL
0
2
4.54
0.3710
8.1696
6
C1
26
CONTROL
0
2
138.02
3.7682
2.7301
7
C1
27
CONTROL
0
2
53.28
3.2616
6.1219
8
C1
28
CONTROL
0
2
28.71
1.6017
5.5786
9
C1
29
CONTROL
0
2
61.51
6.6017
10.7327
10
C1
30
CONTROL
0
2
31.80
3.7195
11.6980
11
C1
31
CONTROL
0
2
82.95
3.5850
4.3219
12
C1
32
CONTROL
0
2
13.51
0.1155
0.8547
13
C1
33
CONTROL
0
2
93.41
6.4528
6.9083
14
C1
34
CONTROL
0
2
8.99
0.0119
0.1329
15
C1
35
CONTROL
0
2
.
.
.

16
C1
36
CONTROL
0
2
84.56
1.5973
1.8888
17
C1
37
CONTROL
0
2
11.18
0.2770
2.4780
18
C1
38
CONTROL
0
2
52.10
4.7007
9.0231
19
C1
39
CONTROL
0
2
.
.
.

20
C1
40
CONTROL
0
2
68.07
5.0388
7.4027
21
C3
21
CONTROL
0
2
185.19
0.1718
0.0928
22
C3
22
CONTROL
0
2
50.45
3.9401
7.8098
23
C3
23
CONTROL
0
2
725.37
3.2272
0.4449
24
C3
24
CONTROL
0
2
328.68
8.1464
2.4785
25
C3
25
CONTROL
0
2
1409.86
50.6053
3.5894
26
C3
26
CONTROL
0
2
113.26
2.0057
1.7709
27
C3
27
CONTROL
0
2
59.76
2.5647
4.2918
28
C3
28
CONTROL
0
2
308.24
0.6218
0.2017
29
C3
29
CONTROL
0
2
120.86
2.6017
2.1527
30
C3
30
CONTROL
0
2
102.66
0.3044
0.2965
31
C3
31
CONTROL
0
2
790.03
4.1639
0.5271
32
C3
32
CONTROL
0
2
357.96
8.6876
2.4269
33
C3
33
CONTROL
0
2
886.19
0.6158
0.0695
34
C3
34
CONTROL
0
2
130.78
1.5195
1.1619
35
C3
35
CONTROL
0
2
47.21
1.4813
3.1376
36
C3
36
CONTROL
0
2
182.98
1.6464
0.8998
37
C3
37
CONTROL
0
2
70.96
1.4720
2.0742
38
C3
38
CONTROL
0
2
215.63
4.4122
2.0462
39
C3
39
CONTROL
0
2
279.03
1.4497
0.5195
40
C3
40
CONTROL
0
2
194.26
5.8231
2.9975
41
C6
21
CONTROL
0
2
33.45
0.3585
1.0717
42
C6
22
CONTROL
0
2
29.55
0.4214
1.4258
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43
C6
23
CONTROL
0
2
4.03
0.3213
7.9645
44
C6
24
CONTROL
0
2
.
.
.

45
C6
25
CONTROL
0
2
19.69
0.7304
3.7097
46
C6
26
CONTROL
0
2
3.07
0.8702
28.3025
47
C6
27
CONTROL
0
2
0.23
0.0352
15.1253
48
C6
28
CONTROL
0
2
24.29
0.4593
1.8907
49
C6
29
CONTROL
0
2
.
.
.

50
C6
30
CONTROL
0
2
31.64
4.3236
13.6663
51
C6
31
CONTROL
0
2
.
.
.

52
C6
32
CONTROL
0
2
5.482
0.2420
4.4148
53
C6
33
CONTROL
0
2
4.245
0.5198
12.2429
54
C6
34
CONTROL
0
2
23.058
0.9027
3.9149
55
C6
35
CONTROL
0
2
24.524
0.0634
0.2584
56
C6
36
CONTROL
0
2
.
.
.

57
C6
37
CONTROL
0
2
22.756
0.0416
0.1830
58
C6
38
CONTROL
0
2
2.335
0.7771
33.2828
59
C6
39
CONTROL
0
2
18.042
3.0613
16.9672
60
C6
40
CONTROL
0
2
2.070
0.1799
8.6880
61
E1
1
ATRAZINE
0
2
78.142
0.2266
0.2899
62
E1
2
ATRAZINE
0
2
465.218
9.6807
2.0809
63
E1
3
ATRAZINE
0
2
34.272
1.5139
4.4172
64
E1
4
ATRAZINE
0
2
345.492
15.7471
4.5579
65
E1
5
ATRAZINE
0
2
153.642
1.7230
1.1214
66
E1
6
ATRAZINE
0
2
39.003
0.7964
2.0418
67
E3
1
ATRAZINE
0
2
25.565
0.8305
3.2488
68
E3
2
ATRAZINE
0
2
20.220
0.3865
1.9116
69
E3
3
ATRAZINE
0
2
149.341
2.2374
1.4982
Data
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EPA
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01
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MEAN
FEMALE
AROMATASE
ACTIVITY
BY
SITE
AND
ANIMAL
NUMBER
139
Obs
Site
Ind_
No
TREAT
_
TYPE_
_
FREQ_
A
STD
CV
70
E3
4
ATRAZINE
0
2
129.350
0.7513
0.5808
71
E3
5
ATRAZINE
0
2
41.427
0.7490
1.8080
72
E3
6
ATRAZINE
0
2
19.066
0.6227
3.2662
73
E3
7
ATRAZINE
0
2
12.951
2.5439
19.6431
74
E3
8
ATRAZINE
0
2
91.475
2.0786
2.2723
75
E3
9
ATRAZINE
0
2
21.132
0.1224
0.5793
76
E3
10
ATRAZINE
0
2
31.900
0.2137
0.6699
77
E4
1
ATRAZINE
0
2
48.127
4.5793
9.5151
78
E4
2
ATRAZINE
0
2
39.544
3.2288
8.1650
79
E4
3
ATRAZINE
0
2
71.724
0.0110
0.0153
80
E4
4
ATRAZINE
0
2
17.726
2.3697
13.3684
81
E4
5
ATRAZINE
0
2
.
.
.

82
E4
6
ATRAZINE
0
2
78.712
2.6865
3.4131
83
E4
7
ATRAZINE
0
2
35.272
2.6168
7.4189
84
E4
8
ATRAZINE
0
2
137.632
3.3057
2.4019
85
E4
9
ATRAZINE
0
2
205.137
6.1020
2.9746
86
E4
10
ATRAZINE
0
2
143.463
8.7359
6.0893
87
E6
1
ATRAZINE
0
2
84.509
0.1741
0.2060
88
E6
2
ATRAZINE
0
2
61.436
1.5234
2.4797
89
E6
3
ATRAZINE
0
2
52.416
0.7188
1.3713
90
E6
4
ATRAZINE
0
2
56.748
1.8847
3.3211
91
E6
5
ATRAZINE
0
2
74.332
2.6188
3.5231
92
E6
6
ATRAZINE
0
2
105.314
1.3007
1.2351
93
E6
7
ATRAZINE
0
2
44.214
1.0374
2.3463
94
E6
8
ATRAZINE
0
2
162.100
6.6676
4.1133
95
E6
9
ATRAZINE
0
2
26.301
0.1825
0.6937
96
E6
10
ATRAZINE
0
2
247.262
5.6848
2.2991
97
E8
1
ATRAZINE
0
2
66.779
3.0958
4.6359
98
E8
2
ATRAZINE
0
2
41.074
2.6246
6.3900
99
E8
3
ATRAZINE
0
2
117.380
5.7140
4.8679
100
E8
4
ATRAZINE
0
2
288.086
17.8714
6.2035
101
E8
5
ATRAZINE
0
2
838.400
22.9345
2.7355
102
E8
6
ATRAZINE
0
2
775.106
55.8228
7.2020
103
E8
7
ATRAZINE
0
2
142.010
8.6639
6.10087
104
E8
8
ATRAZINE
0
2
616.996
29.9642
4.85647
105
E8
9
ATRAZINE
0
2
183.710
12.7623
6.94694
106
E8
10
ATRAZINE
0
2
436.498
28.6119
6.55487
107
E8
11
ATRAZINE
0
2
355.159
22.3086
6.28129
108
E8
12
ATRAZINE
0
2
407.640
12.3229
3.02299
109
E8
13
ATRAZINE
0
2
.
.
.

110
E8
14
ATRAZINE
0
2
.
.
.

111
E8
15
ATRAZINE
0
2
.
.
.
Data
Evaluation
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EPA
MRID
Number
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Page
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ANOVA
FOR
FEMALE
AROMATASE
ACTIVITY
BETWEEN
SITES
141
The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
Site
8
C1
C3
C6
E1
E3
E4
E6
E8
Number
of
observations
111
NOTE:
Due
to
missing
values,
only
101
observations
can
be
used
in
this
analysis.

Dependent
Variable:
A
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
7
1731846.446
247406.635
6.57
<.
0001
Error
93
3504430.360
37682.047
Corrected
Total
100
5236276.806
R­
Square
Coeff
Var
Root
MSE
A
Mean
0.330740
125.8355
194.1186
154.2639
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
Site
7
1731846.446
247406.635
6.57
<.
0001
Levene's
Test
for
Homogeneity
of
A
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
Site
7
2.256E11
3.222E10
2.23
0.0385
Error
93
1.344E12
1.445E10
Bartlett's
Test
for
Homogeneity
of
A
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
Site
7
156.5
<.
0001
Dunnett's
t
Tests
for
A
Data
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NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
93
Error
Mean
Square
37682.05
Critical
Value
of
Dunnett's
t
2.69659
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
Site
Between
Simultaneous
95%

Comparison
Means
Confidence
Limits
E8
­
C1
291.64
96.56
486.72
***

C3
­
C1
263.87
93.80
433.94
***

E1
­
C1
121.86
­
124.90
368.62
E6
­
C1
27.36
­
179.09
233.82
E4
­
C1
22.27
­
191.43
235.97
E3
­
C1
­
9.86
­
216.31
196.60
C6
­
C1
­
48.57
­
228.43
131.29
Data
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NONPARAMETRIC
COMPARISON
OF
FEMALE
AROMATASE
ACTIVITY
ACROSS
SITES
145
The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
A
Classified
by
Variable
Site
Sum
of
Expected
Std
Dev
Mean
Site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C1
18
756.0
918.0
112.689840
42.000000
C3
20
1505.0
1020.0
117.345643
75.250000
C6
16
227.0
816.0
107.517440
14.187500
E1
6
377.0
306.0
69.606034
62.833333
E3
10
351.0
510.0
87.948849
35.100000
E4
9
451.0
459.0
83.892789
50.111111
E6
10
532.0
510.0
87.948849
53.200000
E8
12
952.0
612.0
95.278539
79.333333
Kruskal­
Wallis
Test
Chi­
Square
55.8636
DF
7
Pr
>
Chi­
Square
<.
0001
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
A
Classified
by
Variable
Site
Sum
of
Expected
Std
Dev
Mean
Site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C1
18
7.0
8.910891
1.932520
0.388889
C3
20
16.0
9.900990
2.012363
0.800000
C6
16
0.0
7.920792
1.843819
0.000000
E1
6
4.0
2.970297
1.193675
0.666667
E3
10
3.0
4.950495
1.508236
0.300000
E4
9
5.0
4.455446
1.438679
0.555556
E6
10
5.0
4.950495
1.508236
0.500000
E8
12
10.0
5.940594
1.633933
0.833333
Median
One­
Way
Analysis
Chi­
Square
31.4784
DF
7
Pr
>
Chi­
Square
<.
0001
Data
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EPA
MRID
Number
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01
Page
142
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MEAN
FEMALE
AROMATASE
ACTIVITY
BY
TREATMENT
147
Obs
TREAT
_
TYPE_
_
FREQ_
A
STD
CV
1
ATRAZINE
0
102
162.106
193.158
119.155
2
CONTROL
0
120
147.438
255.819
173.510
ANOVA
FOR
FEMALE
AROMATASE
ACTIVITY
ACROSS
TREATMENTS
148
The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
TREAT
2
ATRAZINE
CONTROL
Number
of
observations
111
NOTE:
Due
to
missing
values,
only
101
observations
can
be
used
in
this
analysis.

Dependent
Variable:
A
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
5406.778
5406.778
0.10
0.7497
Error
99
5230870.029
52837.071
Corrected
Total
100
5236276.806
R­
Square
Coeff
Var
Root
MSE
A
Mean
0.001033
149.0065
229.8632
154.2639
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
TREAT
1
5406.777677
5406.777677
0.10
0.7497
Levene's
Test
for
Homogeneity
of
A
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
TREAT
1
1.966E10
1.966E10
0.60
0.4416
Error
99
3.26E12
3.293E10
Bartlett's
Test
for
Homogeneity
of
A
Variance
Data
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Source
DF
Chi­
Square
Pr
>
ChiSq
TREAT
1
3.7531
0.0527
Dunnett's
t
Tests
for
A
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
99
Error
Mean
Square
52837.07
Critical
Value
of
Dunnett's
t
1.98422
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
Simultaneous
TREAT
Between
95%
Confidence
Comparison
Means
Limits
CONTROL
­
ATRAZINE
­
14.67
­
105.65
76.32
Data
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MEAN
MALE
AROMATASE
ACTIVITY
BY
SITE
AND
ANIMAL
NUMBER
152
Obs
Site
Ind_
No
TREAT
_
TYPE_
_
FREQ_
A
STD
CV
1
C1
1
CONTROL
0
2
.
.
.

2
C1
2
CONTROL
0
2
.
.
.

3
C1
3
CONTROL
0
2
.
.
.

4
C1
4
CONTROL
0
2
.
.
.

5
C1
5
CONTROL
0
2
.
.
.

6
C1
6
CONTROL
0
2
.
.
.

7
C1
7
CONTROL
0
2
.
.
.

8
C1
8
CONTROL
0
2
.
.
.

9
C1
9
CONTROL
0
2
.
.
.

10
C1
10
CONTROL
0
2
.
.
.

11
C1
11
CONTROL
0
2
.
.
.

12
C1
12
CONTROL
0
2
.
.
.

13
C1
13
CONTROL
0
2
.
.
.

14
C1
14
CONTROL
0
2
.
.
.

15
C1
15
CONTROL
0
2
.
.
.

16
C1
16
CONTROL
0
2
.
.
.

17
C1
17
CONTROL
0
2
.
.
.

18
C1
18
CONTROL
0
2
.
.
.

19
C1
19
CONTROL
0
2
.
.
.

20
C1
20
CONTROL
0
2
.
.
.

21
C3
1
CONTROL
0
2
.
.
.

22
C3
2
CONTROL
0
2
.
.
.

23
C3
3
CONTROL
0
2
21.1445
.
.

24
C3
4
CONTROL
0
2
22.9172
13.5041
58.926
25
C3
5
CONTROL
0
2
20.3058
6.1536
30.305
26
C3
6
CONTROL
0
2
24.3767
22.0849
90.598
27
C3
7
CONTROL
0
2
22.2198
2.3277
10.476
28
C3
8
CONTROL
0
2
16.0041
12.6224
78.870
29
C3
9
CONTROL
0
2
6.8434
.
.

30
C3
10
CONTROL
0
2
22.5209
11.1054
49.311
31
C3
11
CONTROL
0
2
.
.
.

32
C3
12
CONTROL
0
2
5.9473
0.8273
13.910
33
C3
13
CONTROL
0
2
10.6430
12.5429
117.851
34
C3
14
CONTROL
0
2
52.5030
44.8708
85.463
35
C3
15
CONTROL
0
2
.
.
.

36
C3
16
CONTROL
0
2
4.2133
.
.

37
C3
17
CONTROL
0
2
.
.
.

38
C6
1
CONTROL
0
2
.
.
.

39
C6
2
CONTROL
0
2
.
.
.

40
C6
3
CONTROL
0
2
.
.
.

41
C6
4
CONTROL
0
2
.
.
.

42
C6
5
CONTROL
0
2
.
.
.

43
C6
6
CONTROL
0
2
.
.
.
Data
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EPA
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44
C6
7
CONTROL
0
2
.
.
.

45
C6
8
CONTROL
0
2
.
.
.

46
C6
9
CONTROL
0
2
.
.
.

47
C6
10
CONTROL
0
2
.
.
.

48
C6
11
CONTROL
0
2
.
.
.

49
C6
12
CONTROL
0
2
.
.
.

50
C6
13
CONTROL
0
2
.
.
.

51
C6
14
CONTROL
0
2
.
.
.

52
C6
15
CONTROL
0
2
.
.
.

53
C6
16
CONTROL
0
2
.
.
.

54
C6
17
CONTROL
0
2
.
.
.

55
C6
18
CONTROL
0
2
.
.
.

56
C6
19
CONTROL
0
2
.
.
.

57
C6
20
CONTROL
0
2
.
.
.

58
E1
1
ATRAZINE
0
2
17.134
.
.

59
E1
2
ATRAZINE
0
2
.
.
.

60
E1
3
ATRAZINE
0
2
.
.
.

61
E1
4
ATRAZINE
0
2
.
.
.

62
E1
5
ATRAZINE
0
2
.
.
.

63
E1
6
ATRAZINE
0
2
.
.
.

64
E1
7
ATRAZINE
0
2
.
.
.

65
E1
8
ATRAZINE
0
2
.
.
.

66
E3
1
ATRAZINE
0
2
217.908
.
.

67
E3
2
ATRAZINE
0
2
.
.
.

68
E3
3
ATRAZINE
0
2
1.978
.
.

69
E3
4
ATRAZINE
0
2
0.675
.
.

70
E3
5
ATRAZINE
0
2
9.357
9.5111
101.647
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MEAN
MALE
AROMATASE
ACTIVITY
BY
SITE
AND
ANIMAL
NUMBER
153
Obs
Site
Ind_
No
TREAT
_
TYPE_
_
FREQ_
A
STD
CV
71
E3
6
ATRAZINE
0
2
.
.
.

72
E3
7
ATRAZINE
0
2
2.707
.
.

73
E3
8
ATRAZINE
0
2
.
.
.

74
E3
9
ATRAZINE
0
2
6.979
.
.

75
E3
10
ATRAZINE
0
2
.
.
.

76
E4
1
ATRAZINE
0
2
19.360
7.9034
40.823
77
E4
2
ATRAZINE
0
2
12.020
2.7125
22.567
78
E4
3
ATRAZINE
0
2
4.930
1.5075
30.578
79
E4
4
ATRAZINE
0
2
11.255
1.5916
14.142
80
E4
5
ATRAZINE
0
2
6.312
1.5449
24.477
81
E4
6
ATRAZINE
0
2
.
.
.

82
E4
7
ATRAZINE
0
2
7.929
0.4772
6.018
83
E4
8
ATRAZINE
0
2
44.522
26.0913
58.603
84
E4
9
ATRAZINE
0
2
21.735
2.4152
11.112
85
E4
10
ATRAZINE
0
2
33.740
16.4240
48.678
86
E6
1
ATRAZINE
0
2
.
.
.

87
E6
2
ATRAZINE
0
2
.
.
.

88
E6
3
ATRAZINE
0
2
.
.
.

89
E6
4
ATRAZINE
0
2
.
.
.

90
E6
5
ATRAZINE
0
2
.
.
.

91
E6
6
ATRAZINE
0
2
.
.
.

92
E6
7
ATRAZINE
0
2
.
.
.

93
E6
8
ATRAZINE
0
2
.
.
.

94
E6
9
ATRAZINE
0
2
.
.
.

95
E6
10
ATRAZINE
0
2
.
.
.

96
E8
1
ATRAZINE
0
2
.
.
.

97
E8
2
ATRAZINE
0
2
.
.
.

98
E8
3
ATRAZINE
0
2
.
.
.

99
E8
4
ATRAZINE
0
2
.
.
.

100
E8
5
ATRAZINE
0
2
.
.
.

101
E8
6
ATRAZINE
0
2
.
.
.
Data
Evaluation
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Hormone
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EPA
MRID
Number
458675­
01
Page
147
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152
ANOVA
FOR
MALE
AROMATASE
ACTIVITY
BETWEEN
SITES
154
The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
Site
8
C1
C3
C6
E1
E3
E4
E6
E8
Number
of
observations
101
NOTE:
Due
to
missing
values,
only
28
observations
can
be
used
in
this
analysis.

Dependent
Variable:
A
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
3
2160.42889
720.14296
0.42
0.7416
Error
24
41331.85569
1722.16065
Corrected
Total
27
43492.28458
R­
Square
Coeff
Var
Root
MSE
A
Mean
0.049674
179.2665
41.49892
23.14929
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
Site
3
2160.428887
720.142962
0.42
0.7416
Levene's
Test
for
Homogeneity
of
A
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
Site
2
1.7871E8
89355288
2.78
0.0821
Error
24
7.717E8
32154336
Bartlett's
Test
for
Homogeneity
of
A
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
Site
2
34.2288
<.
0001
Data
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NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
24
Error
Mean
Square
1722.161
Critical
Value
of
Dunnett's
t
2.54459
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
Simultaneous
Site
Between
95%
Confidence
Comparison
Means
Limits
E3
­
C3
20.80
­
32.00
73.60
E4
­
C3
­
1.16
­
47.72
45.41
E1
­
C3
­
2.00
­
111.91
107.91
Data
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NONPARAMETRIC
COMPARISON
OF
MALE
AROMATASE
ACTIVITY
ACROSS
SITES
158
The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
A
Classified
by
Variable
Site
Sum
of
Expected
Std
Dev
Mean
Site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C3
12
199.0
174.00
21.540659
16.583333
E1
1
16.0
14.50
8.077747
16.000000
E3
6
54.0
87.00
17.860571
9.000000
E4
9
137.0
130.50
20.328551
15.222222
Kruskal­
Wallis
Test
Chi­
Square
3.5546
DF
3
Pr
>
Chi­
Square
0.3137
The
NPAR1WAY
Procedure
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
A
Classified
by
Variable
Site
Sum
of
Expected
Std
Dev
Mean
Site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
C3
12
8.0
6.00
1.333333
0.666667
E1
1
1.0
0.50
0.500000
1.000000
E3
6
1.0
3.00
1.105542
0.166667
E4
9
4.0
4.50
1.258306
0.444444
Median
One­
Way
Analysis
Chi­
Square
4.9286
DF
3
Pr
>
Chi­
Square
0.1771
Data
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Page
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MEAN
MALE
AROMATASE
ACTIVITY
BY
TREATMENT
160
Obs
TREAT
_
TYPE_
_
FREQ_
A
STD
CV
1
ATRAZINE
0
88
22.6808
42.1873
186.004
2
CONTROL
0
114
20.3370
17.8629
87.834
ANOVA
FOR
MALE
AROMATASE
ACTIVITY
ACROSS
TREATMENTS
161
The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
TREAT
2
ATRAZINE
CONTROL
Number
of
observations
101
NOTE:
Due
to
missing
values,
only
28
observations
can
be
used
in
this
analysis.

Dependent
Variable:
A
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
338.13513
338.13513
0.20
0.6555
Error
26
43154.14945
1659.77498
Corrected
Total
27
43492.28458
R­
Square
Coeff
Var
Root
MSE
A
Mean
0.007775
175.9896
40.74034
23.14929
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
TREAT
1
338.1351305
338.1351305
0.20
0.6555
Levene's
Test
for
Homogeneity
of
A
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
TREAT
1
40590974
40590974
0.85
0.3663
Error
26
1.2481E9
48003230
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Bartlett's
Test
for
Homogeneity
of
A
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
TREAT
1
17.1290
<.
0001
Dunnett's
t
Tests
for
A
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
26
Error
Mean
Square
1659.775
Critical
Value
of
Dunnett's
t
2.05558
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
TREAT
Between
Simultaneous
95%

Comparison
Means
Confidence
Limits
CONTROL
­
ATRAZINE
­
7.022
­
39.003
24.958
Data
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