Document ID: EPA-HQ-OPP-2006-0689-0006
Agency: epa
Document Type: Supporting & Related Material
Title: 
Posted Date: 2007-01-24T05:00Z

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY

     WASHINGTON, D.C. 20460

										          OFFICE OF PREVENTION,                               
                                                                        
          

                                  PESTICIDES AND TOXIC  		      
SUBSTANCES    

              			 

MAY 25 2006

MEMORANDUM

SUBJECT:	Review of materials submitted by Monsanto Company in support of
a proposed Section G Experimental Use Permit (EUP No: 524-EUP-OT, DP#
324864) for MON 89034, which produces the Cry1A.105 and Cry2Ab2
proteins, MON 88017, which produces Cry3Bb1 protein,  and MON 89034 x
MON 88017 corn. Submission dated November 17, 2005. Final acreage
calculation confirmed in email from Monsanto to Tessa Milofsky on May
25, 2006.

TO:			Mike Mendelsohn, Senior Regulatory Action Leader	

			Microbial Pesticides Branch 

Biopesticides and Pollution Prevention Division (7511C)

FROM:		Tessa Milofsky, M.S. Agronomist				signed

			Microbial Pesticides Branch 

Biopesticides and Pollution Prevention Division (7511C)

PEER REVIEW:	Alan Reynolds, M.S. Entomologist				signed

			Microbial Pesticides Branch

			Biopesticides and Pollution Prevention Division (7511C)

CONCLUSION: 

The requested EUP and corresponding acreage are ACCEPTABLE. It is
recommended that the registrant’s full request of 2,797 and 6,480 EUP
acres for growing seasons 2006-2007 and 2007-2008, respectively, should
be granted.

COMMENTS/RECOMMENDATIONS:

Names and contact information of collaborators must be submitted prior
to experiment initiation.

Containment measures described in the submission are adequate provided
that a tolerance exemption (temporary or permanent) is in place prior to
granting this EUP.

Total seed needed for the requested acreage, and A.I. present in that
seed, was presented as a sum for both growing seasons. It is recommended
that in future submissions, seed and A.I. quantity should be presented
separately for each program year.

Monsanto Company’s acreage request did not include EUP acreage
containing non-PIP plants. However, information provided in the Appendix
(Tables 6A and 10A) enabled the reviewer to recalculate acreage to
include the non-PIP EUP acreage. Since the reviewer’s modifications to
Monsanto Company’s acreage request bring the calculation methods into
compliance with the guidance described above, the EUP acreage presented
in Tables 1 and 2 are acceptable. Future EUP program requests should
include non-PIP plants contained within EUP experiments.

BACKGROUND:

Monsanto Company has submitted materials in support of their request for
an Experimental Use Permit (EUP), covering the period July 2006 through
July 2008, to allow field testing of MON 89034, MON 88017, and MON 89034
x MON 88017 cotton derived corn plants), which contains the inert marker
CP4 EPSPS. The product is said to protect against the larvae of
lepidopteran corn pests. Planting proposed under the EUP will take place
in 29 states and Puerto Rico, and will not exceed 2,336 and 5,949 total
acres for the 2006-2007 and 2007-2008 growing seasons, respectively.

Program Overview

Participant Information

The names, telephone numbers, and mailing addresses of cooperators and
participants who will supervise experimental work covered under this EUP
are listed in the confidential appendix. 

BPPD Review

Participant information provided in the submission is sufficient. Names
and contact information of collaborators must be submitted prior to
experiment initiation. 

Field Trial Containment

The proposed field trial containment program outlined in Monsanto’s
November, 2005 submission states that one or more of the following
measures may be used:

Test plants will be destroyed prior to anthesis.

Test plants will be located at least 660 feet from open-pollinating
conventional corn that is not a part of this research.  

Tassels will be kept bagged prior to anthesis until pollen shed is
complete or until tassels are removed from the plant.  

Test plants will be detasseled prior to the onset of anthesis. 

Test plants may be located within 660 feet of open-pollinating
conventional corn that is not part of this research, provided that the
period of pollen shed for the test plants does not overlap with the
presence of sexually receptive silks of the conventional plants. 

Test plants may be located within 660 feet of conventional corn in a
breeding nursery, provided the ear shoots of the conventional plants are
covered for the period of time during which the silks of the
conventional plants are viable; seed harvested from the covered ears may
then be handled as conventional material for breeding and other research
purposes only.

BPPD Review

Containment measures described in the submission are adequate provided
that a tolerance exemption (temporary or permanent) is in place prior to
granting this EUP. 

Acreage Calculation

Total EUP acreage is equivalent to the sum of the acreage comprised in
EUP test blocks included in this program request. This calculation would
include all MON 89034, MON 88017, and MON 889034 x 88017 test plants,
non-Bt PIP corn plants used for breeding purposes, non-Bt PIP corn
plants included in experimental trials that are not intentional
recipients of the afore mentioned proteins (e.g. isoline control plots),
and associated border rows contained within the test block, including
border rows which outline the perimeter of the test block. Monsanto is
requesting 2797 and 6480 EUP acres, respectively, for the 2006-2007 and
2007-2008 seasons.   

Table 1.  Tabulation of proposed acreage for each state and territory
for

MON 89034, MON 89034 x MON 88017, and MON 88017 for 2006 - 2007.

STATE	MON 89034 	MON 89034 x MON 88017 	MON 88017	

NON-PIP PLANTS*	TOTAL 

AL	7	0	0	4	7

AR	7	0	0	4	7

CA	118	55	15	19	188

CO	12	10	4	3	26

FL	13	0	0	8	13

GA	8	0	0	6	8

HI	347	95	22	76	464

IA	197	112	64	72	373

ID	6	0	1	6	7

IL	142	102	64	57	308

IN	40	22	57	22	119

KS	16	10	3	7	29

KY	5	0	0	4	5

LA	5	0	0	4	5

MD	3	0	0	2	3

MI	75	100	50	26	225

MN	9	2	1	4	12

MO	20	8	3	6	31

MS	11	0	0	6	11

NC	7	0	0	6	7

NE	57	24	58	31	139

OH	18	0	0	6	18

OR	3	0	0	2	3

PA	12	0	0	8	12

PR	67	15	7	31	89

SD	9	5	3	6	17

TN	14	0	0	6	14

TX	14	0	0	8	14

WA	107	55	10	19	172

WI	7	2	1	2	10

Total	1356	617	363	

461	2797

* Non-PIP plants refers to all corn plants included in the experiment,
and in internal and external 

border areas, which do not contain the Bt proteins produced in Events
MON 89034, MON 89034 

x MON 88017, or MON 88017.

Table 2.  Tabulation of proposed acreage for each state and territory
for

MON 89034, MON 89034 × MON 88017, and MON 88017 for 2007 – 2008.

STATE	MON 89034 	MON 89034 x MON 88017 	MON 88017	

NON-PIP PLANTS*	TOTAL

AL	6	0	0	4	6

AR	6	0	0	4	6

CA	422	110	25	30	557

CO	12	10	4	4	26

FL	10	0	0	8	10

GA	8	0	0	6	8

HI	532	160	62	66	754

IA	531	232	239	82	1002

ID	6	0	1	6	7

IL	220	132	239	78	591

IN	122	92	232	28	446

KS	18	10	3	12	31

KY	5	0	0	4	5

LA	5	0	0	4	5

MD	3	0	0	2	3

MI	403	125	225	32	753

MN	10	2	1	5	13

MO	20	3	3	9	26

MS	9	0	0	7	9

NC	6	0	0	6	6

NE	140	97	233	31	470

OH	18	0	0	8	18

OR	2	0	0	2	2

PA	12	0	0	7	12

PR	57	20	7	31	84

SD	10	5	3	7	18

TN	15	0	0	8	15

TX	13	0	0	7	13

WA	912	110	20	30	1042

WI	8	2	1	3	11

Total	3541	1110	1298	

531	6480

* Non-PIP plants refers to all corn plants included in the experiment,
and in internal and external 

border areas, which do not contain the Bt proteins produced in Events
MON 89034, MON 89034 

x MON 88017, or MON 88017.

BPPD Review

The agency requests that registrants use the following guidance to
calculate EUP acreage.

PIP EUP Acreage Calculation Guidance

Total EUP acreage is equivalent to the sum of the acreage contained
within each experiment covered under protocols listed in the EUP program
submission. If a single experiment is divided among multiple locations,
acreage at all locations must be summed to determine total acreage for
the experiment. It is recommended that total EUP acreage should be
broken down into PIP acres and non-PIP acres, since this delineation
will assist the registrant in their efforts to justify the requested EUP
acreage. For the purposes of PIP EUP acreage calculation, PIP plants are
defined as plants containing a PIP and all non-PIP plants that are
intentionally crossed with PIP plants. Non-PIP plants are defined as all
non-PIP containing plants included within the experiment, such as border
row (internal and external) and control plants, that are not intentional
recipients of pollen from PIP-containing plants. Buffer acreage (e.g.
660 feet for corn) is not included in PIP EUP acreage calculation.

Monsanto Company’s acreage request did not include EUP acreage
containing non-PIP plants. However, information provided in the Appendix
(Tables 6A and 10A) enabled the reviewer to recalculate acreage to
include the non-PIP EUP acreage. Since the reviewer’s modifications to
Monsanto Company’s acreage request bring the calculation methods into
compliance with the guidance described above, the EUP acreage presented
in Tables 1 and 2 are acceptable. Future EUP program requests should
include non-PIP plants contained within EUP experiments, as described
above.

Seed and Active Ingredient Quantities

Calculations of seed required for proposed acreage and corresponding
quantity of A.I. present in that seed is presented in Appendix Table 1A.

BPPD Review

Total seed needed for the requested acreage, and A.I. present in that
seed, was presented as a sum for both growing seasons. It is recommended
that in future submissions, seed and A.I. quantity should be presented
separately for each program year. 

Trial Protocols

Trial protocols for the proposed EUP are described below. The submission
included a tables which list, on a state by state basis, the number of
acres and locations where a protocol may be implemented (Tables 3 and 4
and Appendix Tables 2A-10A). Protocol descriptions include information
on objective, experimental design, genotypes, estimated planting and
harvest schedules for each trial, and containment procedures (shipping,
during trials, and post-harvest). Details of these components may be
found in the submission.

Protocol 1 - Breeding and Observation Nursery

Objective: Observe the phenotype of MON 89034 and MON 89034 × MON 88017
corn expressing in various elite germplasm.  Maintain these
biotechnology-derived lines through seed production. 

Description: The test MON 89034 and MON 89034 × MON 88017 corn plants
will be hand pollinated with conventional or other biotechnology-derived
corn.  The test corn will be self-pollinated, backcrossed, or crossed to
other test plants.  Samples may be collected for analyses to determine
phenotype and/or genotype.  

Protocol 2 - Inbred Seed Increase Production

Objective: Produce seed and plant material for further testing and
product development.

Description: The MON 89034 and MON 89034 × MON 88017 corn plants will
be allowed to open pollinate in isolation to generate seed.

Protocol 3 - Line Per Se, Hybrid Yield and Herbicide Tolerance Trials 

Objective: Assess any potential agronomic effects of the genetic
insertion in MON 89034 and any effects of combining MON 89034 with MON
88017 in various genetic backgrounds.  In addition, crops may be sprayed
with glyphosate herbicides to assess the glyphosate tolerance of MON
89034 and MON 89034 x MON 88017.  

Description: Corn will be planted in a randomized design or single large
blocks.  For the evaluation of MON 89034, each line may be represented
by a segregant without the cry1A.105 and cry2Ab2 genes and a segregant
with the cry1A.105 and cry2Ab2 genes in inbred and hybrid forms.  For
the evaluation of MON 89034 × MON 88017, each line may be represented
by a segregant without the cry3Bb1 and cry1A.105 / cry2Ab2 genes and a
segregant with the cry3Bb1 and cry1A.105 / cry2Ab2 genes in inbred and
hybrid forms.  These trials could include up to 100 different lines per
location.  Lines will be evaluated for several agronomic
characteristics, such as early vigor, plant color, plant height, days to
flowering, days to maturity, yield and test weight.  At some sites,
multiple sets of this type of trial may be conducted.  These will be
considered as unique locations for computational purposes.

Protocol 4 - Insect Efficacy Trials

Objective: Assess the efficacy of a) MON 89034 in various genetic
backgrounds against lepidopteran pests of corn and other potential soil
pests of corn, and b) MON 89034 × MON 88017 corn in various genetic
backgrounds against corn borers, corn rootworm, and other potential soil
pests.

Description: The trials will be planted in a randomized design or single
large blocks.  Plants will be evaluated for efficacy against corn
rootworm, several other insect pests and yield.  At some sites, multiple
sets of this type of trial may be conducted.  These will be considered
as unique locations for computational purposes.

Protocol 5 – Product Characterization and Performance Trials

Objective: Produce grain and plant material for product characterization
and performance studies.

Description: MON 89034 and/or MON 89034 x MON 88017 will be either hand-
or open pollinated to generate seed and/or sampled for various types of
laboratory analyses.  Some or all lines may be hand pollinated or open
pollinated with conventional or other biotechnology-derived corn.

Protocol 6 - Insect Resistance Management Trials

Objective: Studies will be performed to assess the level of control that
MON 89034 provides of key lepidopteran pests, potentially including
European corn borer (ECB), corn earworm (CEW), fall armyworm (FAW) and
Diatraea spp.  

Description: Testing will include rating levels of damage and mortality
for the relevant species in naturally and artificially infested field
plots.  These same plots will be used to assess levels of larval
movement among plants for these species under field conditions.  These
data will be used to support the IRM plan for MON 89034.

Protocol 7 - Benefit Trials

Objective: Develop data to assess the benefits of MON 89034 alone and/or
in combination with MON 88017.

Description: Trials will be conducted to assess the benefits conferred
by MON 89034 through the control of ear pests, particularly the
potential for improved grain quality through mycotoxin reduction. 
Trials will be infested with European corn borer, corn earworm, and/or
fall armyworm, followed by ear inoculations with mycotoxin-producing
fungal species such as Aspergillus flavus and Fusarium verticillioides. 
Ears from MON 89034 plants will be assessed for insect damage and mold
incidence and compared to conventional controls.  Mycotoxin
concentrations will be determined from bulked grain samples using GC,
HPLC, or ELISA techniques.

Protocol 8 - Seed Treatment Trials 

Objective: Evaluate effects of diverse chemical seed treatments, in
combination with test corn, on corn rootworm and additional insect
pests.

Description: Test and conventional seed will be coated with a variety of
seed treatments that will contain soil insecticides and/or other insect
deterrents.  Trials will be planted in a randomized design or single
large blocks.  Plants with the various treatments will be evaluated for
efficacy against corn rootworm, other insect pests, and any effects of
the seed treatment on agronomic characteristics, such as germination and
seedling vigor.

Table 3. Requested total acreage for MON 89034, MON 89034 x MON 88017,
MON 88017 and non-Bt corn acreage by protocol for growing season
2006-2007.

PROTOCOL	MON 89034	MON 89034 x MON 88017	MON 88017	NON-PIP PLANTS*	TOTAL

1	319	117	25	91	552

2	537	250	15	92	894

3	215	182	285	123	805

4	211	58	28	92	389

5	8	0	0	3	11

6	27	5	5	26	63

7	29	5	5	24	63

8	10	0	0	10	20

TOTAL	1356	617	363	461	2797

* Non-PIP plants refers to all corn plants included in the experiment,
and in internal and external 

border areas, which do not contain the Bt proteins produced in Events
MON 89034, MON 89034 

x MON 88017, or MON 88017.

Table 4. Requested total acreage for MON 89034, MON 89034 x MON 88017,
MON 88017 and non-Bt corn acreage by protocol for growing season
2007-2008.

PROTOCOL	MON 89034	MON 89034 x MON 88017	MON 88017	NON-PIP PLANTS*	TOTAL

1	327	125	25	99	576

2	2284	450	15	97	2846

3	657	467	1220	178	2522

4	204	58	28	94	384

5	8	0	0	3	11

6	26	5	5	26	62

7	25	5	5	24	59

8	10	0	0	10	20

TOTAL	3541	1110	1298	531	6480

* Non-PIP plants refers to all corn plants included in the experiment,
and in internal and external 

border areas, which do not contain the Bt proteins produced in Events
MON 89034, MON 89034 

x MON 88017, or MON 88017.

BPPD Review

Acreage requests for each protocol are acceptable.

APPENDIX (Tables 1A-10A)

Table 1A.  Tabulation of proposed active ingredient (a.i.) quantities
for each state and territory

for MON 89034 × MON 88017, MON 89034 and MON 88017 for 2006 through
2008.

 	Planting Seed (lb)    2006-2008	A.I. in Planting Seed (g)            
                                                               2006-2008

STATE	Total	Cry1A.105a	Cry2Ab2b	Cry3Bb1c

AL	260	0.42	0.36	0.00

AR	260	0.42	0.36	0.00

CA	14900	22.56	19.74	41.00

CO	1040	1.41	1.23	5.60

FL	460	0.74	0.64	0.00

GA	320	0.51	0.45	0.00

HI	24360	36.29	31.75	67.80

IA	27500	34.30	30.02	129.40

ID	280	0.38	0.34	0.40

IL	17980	19.07	16.69	107.40

IN	11300	8.83	7.73	80.60

KS	1200	1.73	1.51	5.20

KY	200	0.32	0.28	0.00

LA	200	0.32	0.28	0.00

MD	120	0.19	0.17	0.00

MI	19560	22.50	19.68	100.00

MN	500	0.74	0.64	1.20

MO	1140	1.63	1.43	3.40

MS	400	0.64	0.56	0.00

NC	260	0.42	0.36	0.00

NE	12180	10.18	8.90	82.40

OH	720	1.15	1.01	0.00

OR	100	0.16	0.14	0.00

PA	480	0.77	0.67	0.00

PR	3460	5.09	4.45	9.80

SD	700	0.93	0.81	3.20

TN	580	0.93	0.81	0.00

TX	540	0.86	0.76	0.00

WA	24280	37.89	33.15	39.00

WI	420	0.61	0.53	1.20

Total	165700	211.97	185.47	677.60

 	Pounds (lbs)	0.47	0.41	1.49

a Based on a maximum of 0.00036% of Cry1A.105 protein in corn seed (dry
wt. basis); total values may differ slightly from the sum of individual
values due to rounding.  The products that contain Cry1A.105 are MON
89034 and MON 89034 x MON 88017.

b Based on a maximum of 0.00030% of Cry2Ab2 protein in corn seed (dry
wt. basis); total values may differ slightly from the sum of individual
values due to rounding.  The products that contain Cry2Ab2 are MON 89034
and MON 89034 x MON 88017.

c Based on a maximum of 0.0022% of Cry3Bb1 protein in corn seed (dry wt.
basis); total values may differ slightly from the sum of individual
values due to rounding.   The products that contain Cry3Bb1 are MON
88017 and MON 89034 x MON 88017.Table 2A.   Requested total acreage for
MON 89034 × MON 88017, MON 89034, MON 88017 and border areas (to be
planted with 

non-regulated corn) by trial type for each state and territory for 2006
through 2008.

2006-2008 Total Acres by Trial Type (Protocol):

STATE	1	2	3	4	5	6	7	8	Total

AL	0	0	0	12	0	0	9	0	21

AR	0	0	0	12	0	9	0	0	21

CA	0	707	87	0	0	0	0	0	794

CO	0	0	14	45	0	0	0	0	59

FL	9	0	9	12	0	0	9	0	39

GA	0	0	0	12	0	8	8	0	28

HI	439	755	160	0	6	0	0	0	1360

IA	238	573	598	88	0	12	12	8	1529

ID	8	10	8	0	0	0	0	0	26

IL	232	44	637	79	10	12	12	8	1034

IN	0	0	542	53	0	4	8	8	615

KS	0	0	31	36	0	8	4	0	79

KY	0	0	0	10	0	0	0	8	18

LA	0	0	0	10	0	0	8	0	18

MD	0	0	0	10	0	0	0	0	10

MI	0	474	552	10	0	0	0	0	1036

MN	0	0	0	26	0	8	0	0	34

MO	0	0	26	26	0	8	12	0	72

MS	0	0	0	17	0	8	8	0	33

NC	0	0	0	9	0	8	8	0	25

NE	33	0	535	87	0	12	4	0	671

OH	0	0	19	23	0	0	0	8	50

OR	0	0	0	9	0	0	0	0	9

PA	0	0	0	22	0	8	9	0	39

PR	169	0	0	60	6	0	0	0	235

SD	0	0	8	28	0	12	0	0	48

TN	0	0	15	20	0	8	0	0	43

TX	0	0	0	31	0	0	11	0	42

WA	0	1177	86	0	0	0	0	0	1263

WI	0	0	0	26	0	0	0	0	26

Total	1128	3740	3327	773	22	125	122	40	9277

	

Table 3A.  Requested acreage for MON 89034 by trial type for each state
and territory for 2006 through 2007.

2006-2007 Acres by Trial Type (Protocol):

STATE	1	2	3	4	5	6	7	8	Total

AL	 	 	 	4	 	 	3	 	7

AR	 	 	 	4	 	3	 	 	7

CA	 	110	8	 	 	 	 	 	118

CO	 	 	 	12	 	 	 	 	12

FL	3	 	3	4	 	 	3	 	13

GA	 	 	 	4	 	2	2	 	8

HI	140	200	5	 	2 	 	 	 	347

IA	63	63	43	22	 	2	2	2	197

ID	2	2	2	 	 	 	 	 	6

IL	63	12	42	15	4 	2	2	2	142

IN	 	 	28	8	 	 	2	2	40

KS	 	 	7	7	 	2	 	 	16

KY	 	 	 	3	 	 	 	2	5

LA	 	 	 	3	 	 	2	 	5

MD	 	 	 	3	 	 	 	 	3

MI	 	50	22	3	 	 	 	 	75

MN	 	 	 	7	 	2	 	 	9

MO	 	 	8	8	 	2	2	 	20

MS	 	 	 	7	 	2	2	 	11

NC	 	 	 	3	 	2	2	 	7

NE	8	 	25	22	 	2	 	 	57

OH	 	 	8	8	 	 	 	2	18

OR	 	 	 	3	 	 	 	 	3

PA	 	 	 	7	 	2	3	 	12

PR	40	 	 	25	2	 	 	 	67

SD	 	 	 	7	 	2	 	 	9

TN	 	 	7	5	 	2	 	 	14

TX	 	 	 	10	 	 	4	 	14

WA	 	100	7	 	 	 	 	 	107

WI	 	 	 	7	 	 	 	 	7

Total	319	537	215	211	8	27	29	10	1356

	Table 4A.   Requested acreage for MON 89034 × MON 88017 by trial type
for each state and territory for 2006 through 2007.

2006-2007 Acres by Trial Type (Protocol):

STATE	1	2	3	4	5	6	7	8	Total

AL	 	 	 	 	 	 	 	 	0

AR	 	 	 	 	 	 	 	 	0

CA	 	50	5	 	 	 	 	 	55

CO	 	 	5	5	 	 	 	 	10

FL	 	 	 	 	 	 	 	 	0

GA	 	 	 	 	 	 	 	 	0

HI	40	50	5	 	 	 	 	 	95

IA	30	50	20	10	 	1	1	 	112

ID	 	 	 	 	 	 	 	 	0

IL	30	 	60	10	 	1	1	 	102

IN	 	 	10	10	 	1	1	 	22

KS	 	 	5	5	 	 	 	 	10

KY	 	 	 	 	 	 	 	 	0

LA	 	 	 	 	 	 	 	 	0

MD	 	 	 	 	 	 	 	 	0

MI	 	50	50	 	 	 	 	 	100

MN	 	 	 	2	 	 	 	 	2

MO	 	 	5	2	 	 	1	 	8

MS	 	 	 	 	 	 	 	 	0

NC	 	 	 	 	 	 	 	 	0

NE	2	 	10	10	 	1	1	 	24

OH	 	 	 	 	 	 	 	 	0

OR	 	 	 	 	 	 	 	 	0

PA	 	 	 	 	 	 	 	 	0

PR	15	 	 	 	 	 	 	 	15

SD	 	 	2	2	 	1	 	 	5

TN	 	 	 	 	 	 	 	 	0

TX	 	 	 	 	 	 	 	 	0

WA	 	50	5	 	 	 	 	 	55

WI	 	 	 	2	 	 	 	 	2

Total	117	250	182	58	0	5	5	0	617

	Table 5A.  Requested acreage for MON 88017 by trial type for each
state and territory for 2006 through 2007.

2006-2007 Acres by Trial Type (Protocol):

STATE	1	2	3	4	5	6	7	8	Total

AL	 	 	 	 	 	 	 	 	0

AR	 	 	 	 	 	 	 	 	0

CA	 	5	10	 	 	 	 	 	15

CO	 	 	2	2	 	 	 	 	4

FL	 	 	 	 	 	 	 	 	0

GA	 	 	 	 	 	 	 	 	0

HI	7	5	10	 	 	 	 	 	22

IA	5	2	50	5	 	1	1	 	64

ID	 	1	 	 	 	 	 	 	1

IL	5	2	50	5	 	1	1	 	64

IN	 	 	50	5	 	1	1	 	57

KS	 	 	1	2	 	 	 	 	3

KY	 	 	 	 	 	 	 	 	0

LA	 	 	 	 	 	 	 	 	0

MD	 	 	 	 	 	 	 	 	0

MI	 	 	50	 	 	 	 	 	50

MN	 	 	 	1	 	 	 	 	1

MO	 	 	1	1	 	 	1	 	3

MS	 	 	 	 	 	 	 	 	0

NC	 	 	 	 	 	 	 	 	0

NE	1	 	50	5	 	1	1	 	58

OH	 	 	 	 	 	 	 	 	0

OR	 	 	 	 	 	 	 	 	0

PA	 	 	 	 	 	 	 	 	0

PR	7	 	 	 	 	 	 	 	7

SD	 	 	1	1	 	1	 	 	3

TN	 	 	 	 	 	 	 	 	0

TX	 	 	 	 	 	 	 	 	0

WA	 	 	10	 	 	 	 	 	10

WI	 	 	 	1	 	 	 	 	1

Total	25	15	285	28	0	5	5	0	363

Table 6A.  Requested acreage for border areas (to be planted with
non-regulated corn) by trial type for each state and territory for 2006 

through 2007.

2006-2007 Acres by Trial Type (Protocol):

STATE	1	2	3	4	5	6	7	8	Total

AL	 	 	 	2	 	 	2	 	4

AR	 	 	 	2	 	2	 	 	4

CA	 	12	7	 	 	 	 	 	19

CO	 	 	 	3	 	 	 	 	3

FL	2	 	2	2	 	 	2	 	8

GA	 	 	 	2	 	2	2	 	6

HI	30	30	15	 	1 	 	 	 	76

IA	18	18	22	8	 	2	2	2	72

ID	2	2	2	 	 	 	 	 	6

IL	14	6	22	8	1 	2	2	2	57

IN	 	 	17	3	 	 	 	2	22

KS	 	 	 	3	 	2	2	 	7

KY	 	 	 	2	 	 	 	2	4

LA	 	 	 	2	 	 	2	 	4

MD	 	 	 	2	 	 	 	 	2

MI	 	12	12	2	 	 	 	 	26

MN	 	 	 	2	 	2	 	 	4

MO	 	 	 	2	 	2	2	 	6

MS	 	 	 	2	 	2	2	 	6

NC	 	 	 	2	 	2	2	 	6

NE	5	 	17	7	 	2	 	 	31

OH	 	 	 	4	 	 	 	2	6

OR	 	 	 	2	 	 	 	 	2

PA	 	 	 	4	 	2	2	 	8

PR	20	 	 	10	1 	 	 	 	31

SD	 	 	 	4	 	2	 	 	6

TN	 	 	 	4	 	2	 	 	6

TX	 	 	 	6	 	 	2	 	8

WA	 	12	7	 	 	 	 	 	19

WI	 	 	 	2	 	 	 	 	2

Total	91	92	123	92	3	26	24	10	461

	

Table 7A.  Requested acreage for MON 89034 by trial type for each state
and territory for 2007 through 2008.

2007-2008 Acres by Trial Type (Protocol):

STATE	1	2	3	4	5	6	7	8	Total

AL	 	 	 	4	 	 	2	 	6

AR	 	 	 	4	 	2	 	 	6

CA	 	410	12	 	 	 	 	 	422

CO	 	 	 	12	 	 	 	 	12

FL	2	 	2	4	 	 	2	 	10

GA	 	 	 	4	 	2	2	 	8

HI	150	340	40	 	2 	 	 	 	532

IA	65	320	120	20	 	2	2	2	531

ID	2	2	2	 	 	 	 	 	6

IL	60	12	120	18	 4	2	2	2	220

IN	 	 	110	8	 	 	2	2	122

KS	 	 	8	8	 	2	 	 	18

KY	 	 	 	3	 	 	 	2	5

LA	 	 	 	3	 	 	2	 	5

MD	 	 	 	3	 	 	 	 	3

MI	 	300	100	3	 	 	 	 	403

MN	 	 	 	8	 	2	 	 	10

MO	 	 	8	8	 	2	2	 	20

MS	 	 	 	5	 	2	2	 	9

NC	 	 	 	2	 	2	2	 	6

NE	8	 	110	20	 	2	 	 	140

OH	 	 	8	8	 	 	 	2	18

OR	 	 	 	2	 	 	 	 	2

PA	 	 	 	8	 	2	2	 	12

PR	40	 	 	15	2 	 	 	 	57

SD	 	 	 	8	 	2	 	 	10

TN	 	 	5	8	 	2	 	 	15

TX	 	 	 	10	 	 	3	 	13

WA	 	900	12	 	 	 	 	 	912

WI	 	 	 	8	 	 	 	 	8

Total	327	2284	657	204	8	26	25	10	3541

	Table 8A.  Requested acreage for MON 89034 × MON 88017 by trial type
for each state and territory for 2007 through 2008.

2007-2008 Acres by Trial Type (Protocol):

STATE	1	2	3	4	5	6	7	8	Total

AL	 	 	 	 	 	 	 	 	0

AR	 	 	 	 	 	 	 	 	0

CA	 	100	10	 	 	 	 	 	110

CO	 	 	5	5	 	 	 	 	10

FL	 	 	 	 	 	 	 	 	0

GA	 	 	 	 	 	 	 	 	0

HI	40	100	20	 	 	 	 	 	160

IA	30	100	90	10	 	1	1	 	232

ID	 	 	 	 	 	 	 	 	0

IL	30	 	90	10	 	1	1	 	132

IN	 	 	80	10	 	1	1	 	92

KS	 	 	5	5	 	 	 	 	10

KY	 	 	 	 	 	 	 	 	0

LA	 	 	 	 	 	 	 	 	0

MD	 	 	 	 	 	 	 	 	0

MI	 	50	75	 	 	 	 	 	125

MN	 	 	 	2	 	 	 	 	2

MO	 	 	 	2	 	 	1	 	3

MS	 	 	 	 	 	 	 	 	0

NC	 	 	 	 	 	 	 	 	0

NE	5	 	80	10	 	1	1	 	97

OH	 	 	 	 	 	 	 	 	0

OR	 	 	 	 	 	 	 	 	0

PA	 	 	 	 	 	 	 	 	0

PR	20	 	 	 	 	 	 	 	20

SD	 	 	2	2	 	1	 	 	5

TN	 	 	 	 	 	 	 	 	0

TX	 	 	 	 	 	 	 	 	0

WA	 	100	10	 	 	 	 	 	110

WI	 	 	 	2	 	 	 	 	2

Total	125	450	467	58	0	5	5	0	1110

	

Table 9A.  Requested acreage for MON 88017 by trial type for each state
and territory for 2007 through 2008.

2007-2008 Acres by Trial Type (Protocol):

STATE	1	2	3	4	5	6	7	8	Total

AL	 	 	 	 	 	 	 	 	0

AR	 	 	 	 	 	 	 	 	0

CA	 	5	20	 	 	 	 	 	25

CO	 	 	2	2	 	 	 	 	4

FL	 	 	 	 	 	 	 	 	0

GA	 	 	 	 	 	 	 	 	0

HI	7	5	50	 	 	 	 	 	62

IA	5	2	225	5	 	1	1	 	239

ID	 	1	 	 	 	 	 	 	1

IL	5	2	225	5	 	1	1	 	239

IN	 	 	225	5	 	1	1	 	232

KS	 	 	1	2	 	 	 	 	3

KY	 	 	 	 	 	 	 	 	0

LA	 	 	 	 	 	 	 	 	0

MD	 	 	 	 	 	 	 	 	0

MI	 	 	225	 	 	 	 	 	225

MN	 	 	 	1	 	 	 	 	1

MO	 	 	1	1	 	 	1	 	3

MS	 	 	 	 	 	 	 	 	0

NC	 	 	 	 	 	 	 	 	0

NE	1	 	225	5	 	1	1	 	233

OH	 	 	 	 	 	 	 	 	0

OR	 	 	 	 	 	 	 	 	0

PA	 	 	 	 	 	 	 	 	0

PR	7	 	 	 	 	 	 	 	7

SD	 	 	1	1	 	1	 	 	3

TN	 	 	 	 	 	 	 	 	0

TX	 	 	 	 	 	 	 	 	0

WA	 	 	20	 	 	 	 	 	20

WI	 	 	 	1	 	 	 	 	1

Total	25	15	1220	28	0	5	5	0	1298

	

Table 10A.  Requested acreage for border areas (to be planted with
non-regulated corn) by trial type for each state and territory for 2007
through 2008.

2007-2008 Acres by Trial Type (Protocol):

STATE	1	2	3	4	5	6	7	8	Total

AL	 	 	 	2	 	 	2	 	4

AR	 	 	 	2	 	2	 	 	4

CA	 	15	15	 	 	 	 	 	30

CO	 	 	 	4	 	 	 	 	4

FL	2	 	2	2	 	 	2	 	8

GA	 	 	 	2	 	2	2	 	6

HI	25	25	15	 	1 	 	 	 	66

IA	22	18	28	8	 	2	2	2	82

ID	2	2	2	 	 	 	 	 	6

IL	25	10	28	8	1 	2	2	2	78

IN	 	 	22	4	 	 	 	2	28

KS	 	 	4	4	 	2	2	 	12

KY	 	 	 	2	 	 	 	2	4

LA	 	 	 	2	 	 	2	 	4

MD	 	 	 	2	 	 	 	 	2

MI	 	12	18	2	 	 	 	 	32

MN	 	 	 	3	 	2	 	 	5

MO	 	 	3	2	 	2	2	 	9

MS	 	 	 	3	 	2	2	 	7

NC	 	 	 	2	 	2	2	 	6

NE	3	 	18	8	 	2	 	 	31

OH	 	 	3	3	 	 	 	2	8

OR	 	 	 	2	 	 	 	 	2

PA	 	 	 	3	 	2	2	 	7

PR	20	 	 	10	 1	 	 	 	31

SD	 	 	2	3	 	2	 	 	7

TN	 	 	3	3	 	2	 	 	8

TX	 	 	 	5	 	 	2	 	7

WA	 	15	15	 	 	 	 	 	30

WI	 	 	 	3	 	 	 	 	3

Total	99	97	178	94	3	26	24	10	531

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