Document ID: EPA-HQ-OPP-2004-0411-0010
Agency: epa
Document Type: Supporting & Related Material
Title: 
Posted Date: 2004-12-21T05:00Z

UNITED
STATES
ENVIRONMENTAL
PROTECTION
AGENCY
WASHINGTON,
D.
C.
20460
OFFICE
OF
PREVENTION,
PESTICIDES
AND
TOXIC
SUBSTANCES
MEMORANDUM
Date:
03­
NOV­
2004
Subject:
Ametryn.
Residue
Chemistry
Considerations
for
Reregistration
Eligibility
Decision.

DP
Barcode:
D307104
Case
No.
2010
PC
Codes:
080801
40
CFR:.
§
180.258
From:
William
H.
Donovan,
Ph.
D.,
Chemist
Reregistration
Branch
3
(
RRB3)
Health
Effects
Division
(
HED)
(
7509C)

Through:
Danette
Drew,
Branch
Senior
Scientist
RRB3/
HED
(
7509C)

Chemistry
Science
Advisory
Council
Health
Effects
Division
(
7509C)

To:
Mark
T.
Howard,
CRM
Reregistration
Branch
3
Special
Review
and
Registration
Division
(
7508C)

This
document
was
originally
prepared
under
contract
by
Dynamac
Corporation
(
1910
Sedwick
Rd.,
Building
100,
Durham,
NC
27713;
submitted
7/
26/
2004).
The
document
has
been
reviewed
by
the
HED
and
revised
to
reflect
current
OPP
policies.
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
2
Executive
Summary
Ametryn
(
2­
ethylamino)­
4­(
isopropylamino)­
6­(
methylthio)­
s­
triazine
is
a
methylthiotriazine
herbicide
registered
for
the
preemergent
control
of
weeds
in
bananas,
corn,
pineapples,
plantains,
and
sugarcane.
Ametryn
is
registered
in
the
U.
S.
to
Syngenta
Crop
Protection,
Inc.
under
the
trade
name
EVIK
®
and
is
formulated
as
an
80%
water
dispersible
granule
(
DF),
which
is
typically
applied
as
a
broadcast
preemergence
and/
or
a
directed
postemergence
application
using
ground
or
aerial
equipment.

The
nature
of
the
residue
in
plants
and
animals
is
adequately
understood
based
on
the
available
corn,
sugarcane,
banana,
goat
and
hen
metabolism
studies.
In
plants,
ametryn
is
extensively
metabolized
by
N­
dealkylation
and
desulfation
(
oxidation/
hydroxylation)
to
a
variety
of
triazine
ring
containing
metabolites.
The
metabolism
of
ametryn
in
animals
also
involves
N­
dealkylation
of
the
isopropyl
and
ethyl
side
chains,
as
well
as
modification
at
the
6­
position,
followed
by
conjugation,
with
the
triazine
ring
structure
remaining
intact.
The
regulated
residues
in
plant
commodities
consist
of
ametryn
per
se.
The
residues
of
concern
for
tolerance
enforcement
and
risk
assessment
(
food
only)
also
consist
of
ametryn
per
se.

Permanent
tolerances
are
established
for
residues
of
ametryn
per
se
in/
on
various
plant
commodities
at
0.25
ppm,
with
the
exceptions
of
corn
forage
and
stover,
each
at
0.5
ppm,
and
cassava
root
at
0.1
ppm
[
40
CFR
§
180.258].
There
are
currently
no
tolerances
for
ametryn
residues
in
livestock
commodities
or
for
inadvertent
residues
in
rotational
crops.

Adequate
methods
are
available
for
enforcing
tolerances
and/
or
collecting
data
on
ametryn
residues
in/
on
plant
and
livestock
commodities.
Two
GC
methods
are
available
for
enforcing
tolerances
of
ametryn
in
plant
commodities
and
are
listed
as
Methods
I
and
A
in
PAM
Vol.
II
(
section
180.258).
Method
I
is
a
GC/
microcoulometric
(
MC)
detection
method
for
determining
ametryn
per
se,
with
a
limit
of
quantitation
(
LOQ)
of
0.05
ppm.
Method
A
is
a
GC/
flame
photometric
detection
(
sulfur
mode,
FPD­
S)
method
for
determining
residues
of
ametryn
and
its
three
thiomethyl
metabolites
(
GS­
11354,
GS­
11355,
and
GS­
26831),
with
a
LOQ
of
0.05
ppm
for
parent
and
0.1
ppm
for
each
metabolite.

Considering
the
data
from
the
available
animal
metabolism
and
feeding
studies
and
the
calculated
maximum
theoretical
dietary
burdens
(
MTDBs)
of
0.15­
0.18
ppm
for
cattle
and
0.04
ppm
for
poultry
and
swine,
HED
concludes
that
there
is
no
reasonable
expectation
of
quantifiable
residues
of
ametryn
occurring
in
livestock
commodities
[
40
CFR
§
180.6(
a)(
3)].
Therefore,
tolerances
for
livestock
commodities
are
not
required
at
the
present
time.

Adequate
field
trial
data
are
available
to
support
the
use
of
ametryn
on
corn
(
field
and
pop),
pineapples,
and
sugarcane
provided
the
appropriate
label
amendments
are
made.
An
adequate
number
of
tests
were
conducted
in
the
appropriate
geographical
regions
using
the
appropriate
formulation
applied
at
the
maximum
use
rate.
These
studies
are
also
supported
by
adequate
storage
stability
data.
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
3
The
available
processing
studies
for
corn
(
5x
rate)
and
pineapple
(
3x
rate)
are
adequate
and
indicate
that
residues
of
ametryn
and
its
three
thiomethyl
metabolites
are
not
likely
to
be
quantifiable
(

0.02
ppm)
in
corn
and
pineapple
processed
fractions
derived
from
crops
treated
at
the
maximum
labeled
rates.
However,
the
available
sugarcane
processing
study
is
not
adequate
because
residues
were
<
LOQ
in
cane
(
RAC)
following
applications
at
only
1x
the
maximum
rate.
A
new
sugarcane
processing
study
is
required.

An
adequate
confined
rotational
crop
study
is
available
and
indicates
that
the
metabolism
in
rotational
crops
is
similar
to
the
primary
crops.
Provided
that
the
required
label
amendments
regarding
minimum
plantback
intervals
(
PHIs)
are
made
to
corn
and
sugarcane
use
directions,
adequate
field
rotational
crop
studies
are
also
available
and
indicate
that
rotational
crop
tolerances
are
not
required.

Residue
Chemistry
Deficiencies
°
The
following
label
amendments
for
uses
on
corn
and
sugarcane
should
be
submitted:
°
Corn
­
remove
sweet
corn
or
provide
residue
data.
°
Corn
­
rotational
crop
restrictions
­
minimum
PBIs
of
3
months
for
small
grains,
10
months
for
spinach
and
potatoes,
and
11
months
for
all
other
crops
following
application
of
ametryn
to
corn.
°
Sugarcane
­
change
the
maximum
application
rate
to
12
lb
ai/
A/
crop
cycle
from
16
lb
ai/
A/
crop
cycle,
or
provide
additional
crop
field
trial
data
to
support
the
16
lb
use
rate.
°
Sugarcane
­
rotational
crop
restriction
­
restrict
rotation
to
soybean,
sorghum,
or
cotton
with
a
PBI
of
11
months
following
application
of
ametryn
to
sugarcane.
No
rotational
crop
restriction
is
needed
for
corn
since
it
is
labeled
as
a
primary
crop
for
ametryn
use.

°
Unless
uses
on
sweet
corn
are
deleted
from
all
end­
use
products
(
EPs),
data
are
required
depicting
residues
of
ametryn
and
its
three
thiomethyl
metabolites
in/
on
sweet
corn
ears
(
kernels
plus
cob
with
husks
removed)
following
treatment
at
the
maximum
labeled
rate.

°
A
new
sugarcane
processing
study
is
required
using
sugarcane
bearing
detectable
residues
or
sugarcane
from
plants
treated
at
5x
the
maximum
label
rate.
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
4
N
N
N
N
H
S
C
H
3
NH
CH
3
CH
3
CH
3
Background
Ametryn,
(
2­
ethylamino)­
4­(
isopropylamino)­
6­(
methylthio)­
s­
triazine,
is
a
selective
methylthiotriazine
herbicide
registered
for
the
control
of
broadleaf
and
grass
weeds
primarily
in
banana,
corn,
pineapple,
plantains
and
sugarcane.
In
the
U.
S.,
ametryn
is
registered
to
Syngenta
Crop
Protection,
Inc.
(
the
basic
producer)
under
the
trade
name
EVIK
®
and
is
formulated
as
a
80%
water
dispersible
granule
(
DF).
This
formulation
can
be
applied
as
a
broadcast
preemergence
and/
or
a
directed
postemergence
application
using
ground
or
aerial
equipment.

Ametryn
is
a
FIFRA
List
B
pesticide
that
was
the
subject
of
a
Phase
4
Review,
dated
11/
19/
90,
and
a
Reregistration
Update,
dated
12/
9/
94.
Tolerances
are
currently
established
for
the
residues
of
ametryn
per
se
[
40
CFR
§
180.258],
and
are
set
at
0.25
ppm
for
all
plant
commodities,
with
the
exceptions
of
corn
forage
and
stover,
each
at
0.5
ppm,
and
cassava
root
at
0.1
ppm.
There
are
currently
no
livestock
tolerances
for
ametryn.
The
Ametryn
Update
recommended
that
the
regulated
residues
of
ametryn
should
include
parent
and
its
three
thiomethyl
metabolites
(
GS­
11354,
GS­
11355,
and
GS­
26831),
pending
receipt
and
review
of
oncogenicity
studies
for
ametryn
and
hydroxy
atrazine.
The
Ametryn
Update
also
recommended
that
conclusions
regarding
the
residue
of
concern
should
be
reevaluated
once
these
toxicity
studies
are
available.

Several
data
submissions
have
been
received
and
evaluated
since
the
Ametryn
Update
(
12/
9/
94).
The
information
contained
in
this
document
outlines
the
current
Residue
Chemistry
Science
Assessments
with
respect
to
the
reregistration
of
ametryn.
The
PC
Code
and
nomenclature
of
ametryn
and
its
thiomethyl
metabolites
are
listed
below
in
Table
1
and
the
physicochemical
properties
of
ametryn
are
listed
in
Table
2.

Table
1.
Chemical
Structure
and
Nomenclature
for
Ametryn
and
its
Thiomethyl
Metabolites.

Chemical
structure
Common
name
Ametryn
Molecular
Formula
C9H17N5S
Molecular
Weight
227.35
IUPAC
name
N2­
ethyl­
N4­
isopropyl­
6­
methylthio­
1,3,5­
triazine­
2,4­
diamine
CAS
name
N­
ethyl­
N

­
(
1­
methylethyl)­
6­(
methylthio)­
1,3,5­
triazine­
2,4­
diamine
CAS
#
834­
12­
8
PC
Code
080801
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
Table
1.
Chemical
Structure
and
Nomenclature
for
Ametryn
and
its
Thiomethyl
Metabolites.

5
N
N
N
N
H
2
N
S
CH
3
CH
3
CH
3
N
N
N
N
H
2
N
S
CH
3
CH
3
N
N
N
N
H
2
NH
2
S
CH
3
Current
Food/
Feed
Site
Registration
Banana,
Corn,
Pineapple,
and
Sugarcane
Chemical
structure
Common
name
NA
Company
experimental
name
GS­
11354;
CG­
3
IUPAC
name
NA
CAS
name
N­
isopropyl­
6­(
methylthio)­
1,3,5­
triazine­
2,4­
diamine
CAS
registry
number
4147­
57­
3
Chemical
structure
Common
name
NA
Company
experimental
name
GS­
11355;
CG­
4
IUPAC
name
NA
CAS
name
N­
ethyl­
6­(
methylthio)­
1,3,5­
triazine­
2,4­
diamine
CAS
registry
number
4147­
58­
4
Chemical
structure
Common
name
NA
Company
experimental
name
GS­
26831;
CG­
2
IUPAC
name
NA
CAS
name
6­(
methylthio)­
1,3,5­
triazine­
2,4­
diamine
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
Table
1.
Chemical
Structure
and
Nomenclature
for
Ametryn
and
its
Thiomethyl
Metabolites.

6
CAS
registry
number
5397­
01­
3
Table
2.
Physicochemical
Properties
of
Ametryn
Parameter
Value
Reference
Melting
point
84.5­
86

C
MRID
40877301;
Ametryn
PC
RED
Chapter
pH
8­
9
at
20

C
(
1%
solution
in
water)

Density,
bulk
density,
or
specific
gravity
0.373
g/
mL
at
20

C
Water
solubility
0.020
g/
100
mL
at
20

C
Solvent
solubility
56.9
g/
100
mL
in
acetone
61.4
g/
100/
mL
in
methylene
chloride
51.6
g/
100
mL
in
methanol
46.0
g/
100
mL
in
toluene
24.2
g/
100
mL
in
n­
octanol
1.4
g/
100mL
in
n­
hexane
Vapor
pressure
2.74
x
10­
6
mm
Hg
at
25

C
Dissociation
constant,
pKa
4.02
at
20

C
Octanol/
water
partition
coefficient
KOW
=
423
(
log
P
=
2.63)

UV/
visible
absorption
spectrum
Not
available
RESIDUE
CHARACTERIZATION
General
Discussion
on
Residue
Chemistry
of
Ametryn
860.1200
Directions
for
Use
Product
List
A
query
of
HED's
OPPIN
database
conducted
on
2/
10/
04
identified
three
end­
use
products
(
EPs)
for
ametryn
with
feed/
food
uses
(
Table
3).
Only
one
of
these
products
(
80%
DF)
is
registered
to
the
Basic
producer,
Syngenta
Crop
Protection
(
formerly
Novartis).
The
other
two
labels
are
an
80%
wettable
powder
(
WP)
registered
to
Marman
USA,
Inc.
and
a
4
lb/
gal
flowable
concentrate
(
FlC)
registered
to
Micro
Flo
Co.

Table
3.
Ametryn
End­
use
Products
(
EPs)
with
Registered
Food/
Feed
Uses
EPA
Reg.
No.
Formulation
Registrant
Approved
Label
Date
Product
Name
100­
786
80%
DF
Syngenta
3/
16/
04
Evik
®
DF
Herbicide
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
Table
3.
Ametryn
End­
use
Products
(
EPs)
with
Registered
Food/
Feed
Uses
EPA
Reg.
No.
Formulation
Registrant
Approved
Label
Date
Product
Name
7
48273­
3
80%
WP
Marman
USA,
Inc.
8/
21/
01
Ametryne
80W
51036­
105
4
lb/
gal
FlC
Micro
Flo
Co.
11/
04/
96
Ametryne
4FL
Herbicide
Use
Pattern
Table
A
comprehensive
summary
of
the
registered
food/
feed
use
patterns
of
ametryn,
as
prepared
by
BEAD,
is
presented
in
Appendix
1.
A
tabular
summary
of
the
residue
chemistry
science
assessments
for
reregistration
of
ametryn
is
presented
in
Table
5.
The
conclusions
listed
in
Table
5
regarding
the
reregistration
eligibility
of
ametryn
food/
feed
uses
are
based
on
the
use
patterns
supported
by
the
basic
producer,
Syngenta
Crop
Protection.
When
end­
use
product
DCIs
are
developed,
RD
should
require
that
all
end­
use
product
labels
(
e.
g.,
MAI
labels,
SLNs,
and
products
subject
to
the
generic
data
exemption)
be
amended
such
that
they
are
consistent
with
the
basic
producer's
labels.
Based
on
the
available
field
trial
data,
the
following
amendments
should
be
made
to
all
end­
use
products:

°
In
a
letter
dated
3/
15/
99
with
accompanying
draft
labels,
Syngenta
indicated
that
the
use
on
sweet
corn
was
being
deleted
from
the
label
and
the
use
on
corn
(
field
and
pop)
was
being
amended
to
specify
one
application
per
growing
season.
However,
the
most
recent
version
of
Syngenta's
80%
DF
label
still
includes
the
use
on
sweet
corn
and
does
not
include
a
restriction
to
one
application
per
season
on
corn.
Uses
on
sweet
corn
should
be
removed
from
ametryn
EP
labels,
and
the
use
on
corn
should
be
restricted
to
a
single
application
per
season.

°
As
indicated
on
Syngenta's
draft
label
of
3/
19/
99,
the
total
use
rate
for
sugarcane
should
be
restricted
to
a
maximum
of
12
lb
ai/
A/
crop
cycle
at
all
locations.
(
Use
directions
currently
allow
for
up
to
16
lb
ai/
A/
crop
in
PR).

°
The
label
should
be
amended
to
restrict
rotation
to
either
soybean,
sorghum,
or
cotton
with
a
PBI
of
11
months
following
application
of
ametryn
to
sugarcane
(
see
Field
Rotational
Crop
Section).
No
rotational
crop
restriction
is
needed
for
corn
since
it
is
labeled
as
a
primary
crop
for
ametryn
use.

°
The
available
rotational
crop
field
trial
data
will
support
amending
labels
to
allow
minimum
rotational
crop
PBIs
of
3
months
for
small
grains,
10
months
for
spinach
and
potatoes,
and
11
months
for
all
other
crops
following
application
of
ametryn
to
corn.
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
8
860.1300
Nature
of
the
Residue
­
Plants
44107901.
mem.
wpd
The
qualitative
nature
of
ametryn
residues
in
corn,
pineapple,
and
sugarcane
is
understood
based
on
the
adequate
corn,
sugarcane,
and
banana
metabolism
studies.
The
currently
regulated
residues
in
plant
commodities
consist
of
ametryn
per
se.
The
Ametryn
Update
recommended
that
the
regulated
residues
of
ametryn
should
include
parent
and
its
three
thiomethyl
metabolites
(
GS­
11354,
GS­
11355,
and
GS­
26831),
pending
receipt
and
review
of
oncogenicity
studies
for
ametryn
and
hydroxyatrazine.
The
Ametryn
Update
also
recommended
that
conclusions
regarding
the
residue
of
concern
should
be
reevaluated
once
these
toxicity
studies
are
available.

Hydroxyatrazine
has
not
been
classified
as
to
its
carcinogenic
potential
by
the
HED
Cancer
Peer
Review
Committee.
The
HED
Metabolism
Committee
concluded
in
a
September
29,
1995
meeting
that:
"
For
Atrazine,
the
residues
of
concern
for
cancer
dietary
risk
are
parent
and
chloro
metabolites".
Hydroxyatrazine
is
not
a
chlorometabolite
and
is
not
considered
by
the
HED
Metabolism
Committee
to
possess
carcinogenic
potential
(
HED
Doc.
No.
014312,
R.
Hawks,
28­
AUG­
2000).

The
Cancer
Assessment
Review
Committee
(
CARC)
met
to
evaluate
the
carcinogenic
potential
of
ametryn
on
21­
JUL­
2004
and
concluded
that
the
"
data
are
inadequate
for
an
assessment
of
human
carcinogenic
potential".
The
carcinogenicity
study
in
rats
is
unacceptable
because
the
high
dose
was
considered
to
be
excessive.
The
CARC
requested
that
the
carcinogenicity
study
in
rats
be
repeated
at
a
dose
that
is
adequate
to
assess
carcinogenicity
(
Evaluation
of
the
Carcinogenic
Potential
of
Ametryn,
J.
Kidwell,
02­
SEP­
2004).

The
ametryn
risk
assessment
team
has
determined
that
the
regulated
residues
in
corn,
sugarcane,
and
pineapple
will
include
parent
only
for
the
purpose
of
tolerance
enforcement
and
risk
assessment.
The
basis
for
this
decision
is
as
follows:

°
Residue
levels
of
ametryn
and
its
three
thiomethyl
metabolites
are
very
low
in
the
edible
parts
of
crops
that
will
be
treated
with
ametryn.


In
corn
grain,
residue
levels
of
ametryn
and
its
three
thiomethyl
metabolites
were
each
<
0.02
ppm
(
LOQ)
in
29
field
trials
conducted
at
1x,
9
trials
at
2x,
4
trials
at
3x,
and
4
trials
at
5x.


In
pineapple,
residue
levels
of
the
three
thiomethyl
metabolites
were
each
<
0.02
ppm
(
LOQ)
in
8
field
trials
conducted
at
1x,
3
trials
at
2x,
and
2
trials
at
3x.
One
1x
treated
pineapple
sample
was
reported
to
contain
0.05
ppm
ametryn.


In
sugarcane,
residue
levels
of
ametryn
and
its
three
thiomethyl
metabolites
were
each
<
0.02
ppm
(
LOQ)
in
17
field
trials
conducted
at
a
nominal
rate
of
1x.
°
Corn,
sugarcane,
and
banana
metabolism
studies
show
that
the
thiomethyl
metabolites
each
comprise
<
10%
TRR
in
the
edible
parts
of
the
crops
studied.
°
The
toxicological
database
does
not
indicate
a
special
concern
regarding
the
toxicity
of
three
thiomethyl
metabolites.
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
9
In
the
corn
metabolism
study,
[
U­
14C­
triazine]
ametryn
was
applied
postemergence
as
a
basal/
soil
directed
spray
to
greenhouse­
grown
corn
at
4
lb
ai/
A
(
2x
the
maximum
label
rate)
when
plants
were
12­
18
inches
in
height.
Total
radioactive
residues
(
TRR)
were
2.47
ppm
in
forage
at
56
days
after
treatment
(
DAT),
and
4.56
ppm
in
fodder
and
0.16
ppm
in
grain
at
maturity
(
111
DAT).
Organosoluble
14C­
residues
in
immature
forage
declined
from
100%
of
the
TRR
on
the
day
of
application
to
11%
of
the
TRR
in
mature
fodder.
Radioactive
residues
in
immature
forage
were
not
further
analyzed.
At
maturity,
organosoluble
residues
in
fodder
consisted
principally
of
ametryn
(
1.8%
TRR)
and
GS­
11354
(
0.2%
TRR).
In
mature
tissues,
the
majority
of
14C­
residues
consisted
of
aqueous
soluble
(
45­
58%
TRR)
and
insoluble
(
31­
57%
TRR)
residues.
Cation
exchange
chromatography
separated
aqueous
soluble
14C­
residues
from
mature
tissues
into
numerous
distinct
components
each
of
which
accounted
for
<
10%
of
the
TRR,
with
the
exception
of
P3
fractions
from
stalks
and
cobs.
The
cation
exchange
elution
profiles
were
qualitatively
similar
for
14C­
residues
from
stalks,
cobs,
and
grain.
Aqueous
soluble
14C­
residues
(
60.5%
TRR)
in
stalks
with
foliage
were
separated
by
cation
exchange
chromatography
into
14
distinct
components,
with
each
one
accounting
for
0.7­
12.7%
of
the
TRR.
HPLC
and
2D­
TLC
analyses
of
the
isolated
fractions
identified
the
metabolites
GS­
17792
(
1.8%
TRR)
and
GS­
17794
(
3.6%
TRR).
Insoluble
14C­
residues
in
stalks/
foliage
were
solubilized
by
refluxing
in
aqueous
MeOH
and
by
enzymatic
and
acid
hydrolyses,
and
were
polar
in
nature.

In
the
sugarcane
metabolism
study,
[
U­
14C­
triazine]
ametryn
was
applied
to
greenhouse­
grown
sugarcane
as
a
preemergence
application
at
8
lb
ai/
A
and
again
as
soil/
basal
directed
sprays
at
4
lb
ai/
A/
application
at
29
and
50
days
after
planting,
for
a
total
of
16
lb
ai/
A
(
1x
maximum
use
rate).
TRR
in
forage
were
0.12
and
1.57
ppm
in
forage
collected
29
and
50
days
after
the
first
application.
In
separated
stalks
and
foliage,
TRR
were
0.40
and
2.17
ppm,
respectively,
at
84
DAT
and
0.42
and
3.06
ppm
at
202
DAT.
Organosoluble
14C­
residues
in
immature
sugarcane
declined
from
55.2%
of
the
TRR
on
Day­
29
to
28­
32%
of
the
TRR
in
Day­
84
stalks
and
foliage
and
3.4­
7.2%
of
the
TRR
in
mature
(
Day­
202)
stalks
and
foliage.
Organosoluble
residues
were
not
further
analyzed.
Cation
exchange
chromatography
separated
aqueous
soluble
14C­
residues
from
mature
tissues
into
14
distinct
components.
Each
of
these
fractions
accounted
for
<
10%
of
the
TRR,
with
the
exception
of
one
fraction
(
12.6%
TRR)
in
foliage.
Insoluble
14C­
residues
from
mature
tissues
were
released
by
refluxing
in
aqueous
MeOH
and
by
enzymatic
and
acid
hydrolyses.

In
the
banana
metabolism
study,
[
U­
14C­
triazine]
ametryn
was
applied
three
times
as
a
directed
soil
application
to
greenhouse­
grown
banana
plants
at
112­
120
days
retreatment
intervals,
beginning
when
plants
were
3.5
feet
in
height.
TRR
were
0.579
in
immature
leaves
collected
31
days
after
the
second
application
and
1.59
ppm
in
mature
leaves
collected
at
fruit
maturity,
69
days
after
the
second
application.
TRR
in
mature
fruit
(
0.087
ppm)
were
substantially
lower
than
in
leaves
and
were
equally
distributed
between
the
peel
(
0.098
ppm)
and
pulp
(
0.076
ppm).
Methanolic
extraction
of
leaf
and
fruit
samples
released
61.4­
90.9%
of
the
TRR,
and
subsequent
analyses
of
organic
and
aqueous
soluble
14C­
residues
identified
40.6­
61.6%
of
the
TRR
in
leaves
and
58.7­
75.6%
of
the
TRR
in
whole
fruit,
peel
and
pulp
samples.
Including
ametryn,
a
total
of
10
triazine­
ring
containing
compounds
were
identified
in
leaves
and
fruit.
In
mature
leaves,
ametryn
was
detected
at
3.0%
of
the
TRR
and
the
major
metabolite
was
GS­
11957
(
39.7%
TRR);
the
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
10
remaining
8
metabolites
each
accounted
for

5.1%
of
the
TRR
and
included:
GS­
11354,
GS­
26831,
G­
34048,
GS­
17791,
GS­
17792,
GS­
17794,
G­
28521,
and
GS­
12517.
In
whole
fruit,
peel
and
pulp,
the
major
14C­
residues
were
GS­
11957
(
39.3­
56.7%
TRR)
and
G­
28521
(
15.1­
17.2%
TRR);
the
remaining
4
metabolites
each
accounted
for

3.3%
of
the
TRR
and
included:
G­
34048,
GS­
17791,
GS­
17792,
and
GS­
17794.

A
separate
acid
hydrolysis
released
87­
96.1%
of
the
TRR
from
leaves
and
fruit
fractions,
and
subsequent
analyses
identified
6­
7
triazine­
ring
containing
metabolites,
accounting
for
72.9­
79.2%
of
the
TRR.
The
major
acid
hydrolyzed
14C­
residues
in
mature
leaves
were
G­
28521
(
18.2%
TRR),
GS­
17794
(
14.8%
TRR),
G­
34048
(
12.1%
TRR),
and
GS­
11957
(
30.4%
TRR).
The
major
acid
hydrolyzed
residues
in
whole
fruit
and
pulp
were
G­
28521
(
40.7­
49.0%
TRR)
and
GS­
11957
(
19.7­
23.8%
TRR).

Based
on
the
banana
metabolism
study,
for
which
the
most
extensive
data
were
available,
ametryn
is
extensively
metabolized
in
plants
primarily
by
N­
dealkylation
and
desulfation
(
oxidation/
hydroxylation)
to
a
variety
of
triazine
ring
containing
metabolites.
At
least
in
bananas,
glutathione
conjugation
did
not
appear
to
be
an
important
route
of
metabolism.

860.1300
Nature
of
the
Residue
­
Livestock
The
qualitative
nature
of
ametryn
residues
in
poultry
and
ruminants
is
understood
based
on
the
adequate
goat
and
hen
metabolism
studies.
There
are
currently
no
tolerances
for
ametryn
residues
in
livestock
commodities,
and
the
available
metabolism
and
feeding
studies
indicate
that
none
are
presently
required.

The
ametryn
risk
assessment
team
has
determined
that,
if
new
ametryn
uses
are
added
so
that
tolerances
are
needed
for
livestock
commodities,
the
regulated
residues
in
livestock
commodities
should
include
parent
and
its
three
thiomethyl
metabolites
(
GS­
11354,
GS­
11355,
and
GS­
26831)
for
the
purpose
of
tolerance
reassessment
and
risk
assessment.
The
basis
for
this
decision
is
as
follows:

°
Metabolism
studies
in
goats
and
hens
demonstrate
that
ametryn
and
the
thiomethyl
metabolites
comprise
>
10%
TRR
in
several
edible
livestock
matrices.
°
An
adequate
data
collection
method
is
available
(
Method
AG­
649)
for
determination
of
ametryn
and
its
three
thiomethyl
metabolites.
If
necessary,
this
method
could
be
validated
for
tolerance
enforcement
purposes.

In
the
goat
metabolism
study,
two
goats
were
dosed
orally
for
3
days
with
[
U­
14C­
triazine]
ametryn
at
a
level
equivalent
to
50
ppm
in
the
diet,
which
is

280x
the
maximum
theoretical
dietary
burden
(
MTDB)
for
dairy
cattle.
Maximum
14C­
residues
were
attained
in
milk
on
Day
2
at
0.686
or
1.277
ppm.
At
sacrifice,
TRR
were
2.71­
2.89
ppm
in
liver,
3.0­
3.05
ppm
in
kidney,
0.092­
0.137
ppm
in
muscle,
and
0.084­
0.088
ppm
in
fat.
Solvent
extraction
released
84%
of
the
TRR
from
fat,
42­
53%
of
the
TRR
from
milk,
muscle,
and
kidney,
and
29%
of
the
TRR
from
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
11
liver.
Subsequent
acid
hydrolyses
released
the
remaining
radioactivity
from
each
matrix,
and
HPLC
and
TLC
analyses
identified
>
80%
of
the
TRR
in
each
matrix.

The
principal
organosoluble
residues
in
goats
milk
and
tissues
were
ametryn,
GS­
11354,
and
GS­
26831.
Ametryn
accounted
for
40%
of
the
TRR
in
fat,
9.7%
TRR
in
muscle,
and
0.2­
2.3%
TRR
in
liver,
kidney,
and
milk,
and
GS­
11354
accounted
for
20.8%
TRR
in
fat,
11.5%
TRR
in
muscle,
and
1.9­
2.4%
TRR
in
liver,
kidney,
and
milk.
Metabolite
GS­
26831
accounted
for
13.5%
TRR
in
muscle,
7.0­
9.6%
TRR
in
milk
and
kidney,
and
3.3­
4.7%
TRR
in
liver
and
fat.
The
organosoluble
metabolite
GS­
11355
was
also
identified
in
milk
and
all
tissues
at
0.9­
4.6%
of
the
TRR.
The
combine
residues
of
ametryn
its
three
thiomethyl
metabolites
(
GS­
11354,
GS­
11355,
and
GS­
26831)
accounted
for
16.4%
TRR
in
milk,
9.1%
TRR
in
liver,
12.5%
TRR
in
kidney,
37.1%
TRR
in
muscle,
and
71.2%
TRR
in
fat;
actual
levels
of
these
combined
residues
ranged
from
0.03
ppm
in
muscle
to
0.37
ppm
in
kidney.
Other
minor
solvent
extracted
metabolites
containing
the
triazine­
ring
included:
GS­
11794,
GS­
11957,
G­
4048,
BPM­
VI­
31P,
GS­
17791,
and
GS­
17792;
each
of
these
metabolites
were
detected
in
various
tissues
or
milk
at
0.3­
9.8%
TRR.
The
14C­
residues
released
by
acid
hydrolysis
were
attributed
to
2,6­
dihydroxy­
4­
amino­
triazine
(
G­
35713)
at
61%
TRR
78%
TRR
in
liver,
47­
58%
TRR
in
milk,
muscle,
and
kidney,
and
16%
TRR
in
fat.

In
the
poultry
metabolism
study,
10
hens
were
dosed
orally
for
3
days
with
[
U­
14C­
triazine]
ametryn
at
levels
equivalent
to
50
ppm
in
the
diet
(
1,250x
MTDB).
Maximum
14C­
residues
in
eggs
were
attained
on
Day
3
at
0.099
ppm
in
egg
whites
and
0.268
ppm
in
egg
yolks.
At
sacrifice,
TRR
were
4.98
ppm
in
liver,
0.379­
0.558
in
muscle,
0.618
ppm
in
skin,
and
0.240
ppm
in
fat.
Solvent
extraction
released
72
and
58%
of
the
TRR
from
egg
whites
and
fat,
respectively,
and
16­
25%
of
the
TRR
from
the
remaining
matrices.
Subsequent
protease
and
acid
hydrolyses
released
essentially
all
of
the
remaining
radioactivity
from
each
matrix.

The
principal
organosoluble
residues
in
poultry
were
ametryn,
accounting
for
40%
of
the
TRR
in
fat
and
0.3­
2.7%
of
the
TRR
in
skin,
yolks,
and
muscle,
and
GS­
26831,
accounting
for
52%
TRR
in
egg
whites,
10.4%
TRR
in
yolks,
5.7­
8.7%
TRR
in
muscle,
and
1.2­
1.6%
TRR
in
fat
and
liver.
Other
minor
organosoluble
residues
in
eggs
and
tissues
included
GS­
11355
(
0.06­
5.2%
TRR),
GS­
11354
(
0.1­
3.0%
TRR),
G­
34048
(
0.2­
0.4%
TRR),
and
a
sulfoxide
metabolite
(
0.4%
TRR).
The
combine
residues
of
ametryn
its
three
thiomethyl
metabolites
(
GS­
11354,
GS­
11355,
and
GS­
26831)
accounted
for
63.7%
TRR
in
egg
white,
14.3%
TRR
in
yolks,
14­
24%
TRR
in
muscle,
51.7%
TRR
in
fat,
and
7.4%
TRR
in
liver;
actual
levels
of
the
combined
residues
ranged
from
0.03
ppm
in
yolks
to
0.42
ppm
in
liver.
Acid
hydrolysis
of
the
residual
solids
released
the
following
triazine­
ring
containing
metabolites:
G­
35713
at
61%
TRR
in
egg
yolks
and
21­
36%
TRR
in
liver,
muscle,
skin,
and
fat;
G­
28521
at
17­
18%
TRR
in
liver,
muscle,
and
skin;
and
GS­
17792
at
9.4­
21%
TRR
in
liver,
muscle,
and
skin.

The
metabolism
of
ametryn
in
livestock
involves
N­
dealkylation
of
the
isopropyl
and
ethyl
side
chains,
as
well
as
modification
at
the
6­
position,
followed
by
conjugation,
possibly
to
proteins,
with
the
triazine
ring
structure
remaining
intact.
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
12
860.1340
Residue
Analytical
Methods
­
Plants
Tolerance
enforcement
methods:
Two
methods
are
available
for
enforcing
tolerances
of
ametryn
in
plant
commodities
and
are
listed
as
Method
I
and
A
in
PAM
Vol.
II.
Method
I
is
a
GC/
microcoulometric
(
MC)
detection
method
for
determining
ametryn
per
se,
and
Method
A
is
a
GC/
FPD­
S
method
for
determining
residues
of
ametryn
and
its
three
thiomethyl
metabolites
(
GS­
11354,
GS­
11355,
and
GS­
26831).

For
Method
I
(
Method
AG­
63),
residues
are
extracted
from
plant
commodities
with
acetonitrile,
diluted
with
water,
and
partitioned
into
methylene
chloride.
Residues
are
then
cleaned
up
using
an
alumina
column
and
analyzed
by
GC
/
MC.
The
LOQ
for
ametryn
is
0.05
ppm.

For
Method
A
(
Method
AG­
169),
residues
are
extracted
with
chloroform
and
concentrated.
For
analysis
of
ametryn,
extracted
residues
are
redissolved
in
carbon
tetrachloride
and
cleaned
up
using
a
sodium
bisulfate
column.
For
analysis
of
the
metabolites,
extracted
residues
are
dissolved
in
0.5
N
HCl
and
partitioned
against
hexane.
The
aqueous
fraction
is
made
basic
and
metabolite
residues
are
then
partitioned
into
methylene
chloride.
Parent
and
metabolite
residues
are
then
determined
by
GC/
FPD­
S.
The
LOQ
is
0.05
ppm
for
parent
and
0.1
ppm
for
each
metabolite.

In
addition
to
the
above
methods,
a
more
recent
GC/
FPD­
S
method
(
Method
AG­
563)
is
available.
This
method,
which
has
been
used
extensively
for
data
collection,
has
undergone
a
successful
independent
laboratory
method
validation
trial
and
has
been
validated
using
14C­
labeled
samples
from
the
corn
and
sugarcane
metabolism
studies.

For
Method
AG­
563,
residues
of
ametryn
and
its
thiomethyl
metabolites
are
extracted
by
refluxing
in
methanol/
water
(
9:
1,
v/
v)
for
1
hour,
concentrated
to
an
aqueous
solution,
acidified,
and
partitioned
against
hexane.
Residues
in
the
aqueous
solution
are
then
made
alkaline
(
pH
~
10)
and
cleaned
up
using
a
Chem
Elute
extraction
column
eluted
with
methylene
chloride.
Residues
are
concentrated
and
determined
by
GC/
FPD­
S.
The
LOQ
for
each
of
the
four
analytes
is
0.02
ppm.

Data
collection
methods:
Residue
data
from
the
acceptable
field
trials
and
processing
studies
have
been
collected
using
the
adequate
Method
AG­
563,
described
above,
and
a
related
GC/
FPDS
method
(
Method
AG­
581)
that
was
used
for
the
analysis
of
residues
in
soapstock.
For
Method
AG­
581,
soapstock
is
dissolved
in
water,
acidified,
filtered,
and
extracted
with
hexane.
Residues
in
the
remaining
aqueous
phase
are
then
made
alkaline
and
cleaned
up
using
a
Chem
Elut
extraction
column
eluted
with
methylene
chloride.
Residues
are
concentrated
and
determined
by
GC/
FPD­
S.
The
LOQ
for
Method
AG­
581
is
0.05
ppm
for
each
analyte.
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
13
860.1340
Residue
Analytical
Methods
­
Livestock
commodities
44477701.
mem.
wpd
44477702.
mem.
wpd
Tolerance
enforcement
method:
As
tolerances
are
not
currently
necessary
for
livestock
commodities,
an
enforcement
method
is
not
required.

Data
collection
method:
An
adequate
GC/
NPD
method
(
Method
AG­
649)
is
available
for
determining
residues
of
ametryn
and
its
three
thiomethyl
metabolites
(
GS­
11354,
GS­
11355,
and
GS­
26831)
in
livestock
commodities.
For
this
method,
residues
are
extracted
with
methanol/
water
(
tissues
and
egg)
or
acetone/
water
(
milk),
filtered,
and
concentrated.
Residues
are
then
cleaned­
up
using
a
C
18
solid
phase
extraction
cartridge
and
a
Florisil
column.
Residues
are
determined
by
GC/
NPD
with
all
four
analytes
determined
in
a
single
injection,
and
residues
are
express
in
terms
of
each
analyte.
The
LOQ
for
ametryn
and
each
metabolite
is
0.02
ppm
in
tissues
and
eggs
and
0.01
ppm
in
milk.
The
above
method
was
successfully
validated
by
the
developing
laboratory
and
was
radiovalidated
using
milk,
liver,
and
kidney
samples
from
an
14C­
ametryn
dosed
dairy
goat.

860.1360
Multiresidue
Methods
The
FDA
PESTDATA
database
dated
11/
01
(
PAM
Volume
I,
Appendix
I)
indicates
that
ametryn
per
se
is
completely
recovered
using
Multiresidue
Methods
Section
302.
The
Ametryn
Update
also
indicated
that
additional
data
have
been
submitted
examining
the
recovery
of
ametryn
and
its
thiomethyl
metabolites
and
hydroxy
triazine
methods
through
the
Multiresidue
Method
Testing
Protocols.

860.1380
Storage
Stability
43931001.
mem.
wpd
In
support
of
previously
submitted
goat
and
poultry
metabolism
studies,
Syngenta
Crop
Protection,
Inc.
submitted
data
supporting
the
stability
of
14C­
residues
in
sample
matrices
over
the
course
of
these
metabolism
studies.
In
the
goat
study,
samples
were
initially
analyzed
within
3
months
of
sampling,
and
reanalyzed
toward
the
end
of
the
study
after

18
months
of
frozen
storage.
In
the
poultry
study,
samples
were
initially
analyzed
within
5
months
of
sampling,
and
reanalyzed
toward
the
end
of
the
study
after

17
months
of
frozen
storage.
The
supporting
storage
stability
data
indicate
that
the
extractability
of
14C­
residues
and
the
distribution
of
radioactivity
between
solvent
fractions
was
similar
for
each
sample
matrix
at
the
different
storage
intervals.
In
addition,
the
chromatographic
profile
of
selected
solvent
fractions
was
similar
for
the
two
analyses
indicating
that
14C­
residues
were
stable
in
frozen
storage
over
the
course
of
these
studies.

44783701.
mem.
wpd
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
14
The
available
storage
stability
data
for
plant
RACs
and
processed
commodities
are
adequate
and
indicate
that
ametryn
and
its
three
thiomethyl
metabolites
are
stable
at
­
20

C
for
at
least
30
months
in
sugarcane
and
corn
grain,
28
months
in
corn
oil,
20­
24
months
in
corn
forage,
25
months
in
bananas,
and
22
months
in
pineapples,
sugarcane
molasses
and
sugar,
and
corn
meal,
grits,
flour,
and
starch.
These
data
support
the
sample
storage
intervals
in
the
field
trials
for
primary
and
rotational
crops
and
the
processing
studies.

44477704.
mem.
wpd
The
available
interim
storage
stability
data
on
livestock
commodities
indicate
that
residues
of
ametryn
and
its
thiomethyl
metabolites
are
completely
stable
at
­
20

C
in
eggs
and
milk
for
at
least
14
months.
The
three
thiomethyl
metabolites
are
also
relatively
stable
in
frozen
muscle,
with
only
a
15%
decline
occurring
after
14
months
of
storage.
Ametryn
appears
to
be
less
stable
in
muscle,
declining
by
28%
after
14
months.
Residues
of
ametryn
and
all
three
metabolites
declined
steadily
in
liver.
By
the
14­
month
sampling
interval,
residues
of
ametryn
and
its
thiomethyl
metabolites
had
declined
by
20­
34%
in
frozen
liver.
The
current
data
reported
in
the
interim
study
adequately
support
the
cattle
feeding
study,
in
which
samples
of
milk
and
tissues
analyzed
after

10­
12
months
of
storage,
but
do
not
cover
the
maximum
sample
storage
interval
in
the
poultry
feeding
study,
in
which
egg
and
tissue
samples
were
stored
for
18­
20
months
prior
to
analysis.

Conclusions
The
reregistration
requirements
for
storage
stability
data
are
fulfilled
for
plant
and
livestock
commodities.
HED
notes
that
the
interim
storage
stability
data
on
poultry
commodities
covers
storage
intervals
up
to
14
months
while
samples
from
the
livestock
feeding
studies
were
stored
frozen
for
up
to
20
months.
However,
HED
will
not
require
additional
storage
stability
data
for
poultry
at
this
time
for
the
following
reasons:
1)
submitted
data
demonstrate
stability
in
eggs
and
milk
for
at
least
14
months,
and
2)
the
low
poultry
dietary
burden
and
low
transfer
rate
of
residues
to
poultry
tissues
results
in
no
reasonable
expectation
of
finite
residues
in
poultry
tissues
[
40
CFR
180.6(
a)(
3)].
This
conclusion
should
be
reevaluated
if
any
new
ametryn
uses
are
added
that
result
in
an
increase
in
the
dietary
burden
to
poultry.

860.1400
Water,
Fish,
and
Irrigated
Crops
Ametryn
is
not
registered
for
direct
use
on
water
and
aquatic
food
and
feed
crops;
therefore,
no
residue
chemistry
data
are
required
under
these
guideline
topics.

860.1460
Food
Handling
Ametryn
is
not
registered
for
use
in
food­
handling
establishments;
therefore,
no
residue
chemistry
data
are
required
under
this
guideline
topic.

860.1480
Meat,
Milk,
Poultry,
and
Eggs
44477705.
mem.
wpd
44477706.
mem.
wpd
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
15
Based
on
the
use
of
ametryn
on
corn
and
pineapples,
there
is
the
potential
for
exposure
of
livestock
to
ametryn
residues
in
livestock
feed
items.
The
maximum
theoretical
dietary
burdens
(
MTDB)
of
ametryn
residues
for
livestock
are
calculated
below
in
Table
4
considering
the
regulation
of
ametryn
per
se.
Based
on
reassessed
tolerances
for
ametryn
per
se,
the
MTDB
is
0.15
and
0.18
ppm
for
beef
and
dairy
cattle,
respectively,
and
the
MTDB
for
both
poultry
and
swine
is
0.04
ppm.

Table
4.
Calculation
of
Maximum
Theoretical
Dietary
Burdens
of
Livestock
for
Ametryn.
1
Feed
Commodity
%
Dry
Matter1
%
Diet2
Reassessed
Tolerance
(
ppm)
Dietary
Contribution
(
ppm)
3
ametryn
residues
ametryn
residues
Beef
and
Dairy
Cattle
Corn,
forage
40
40/
50
0.10
0.100/
0.125
Corn,
grain
88
20
0.05
0.011
Pineapple,
process
residue
4
25
20
0.05
0.040
TOTAL
BURDEN
0.15/
0.18
5
Poultry
and
Swine
Corn
grain
NA
80
0.05
0.040
TOTAL
BURDEN
0.040
1
The
MTBD
was
calculated
based
on
residues
of
ametryn
per
se.
2
Table
1
(
OPPTS
Guideline
860.1000).
3
Contribution
=
([
tolerance
/%
DM]
X
%
diet)
for
beef
and
dairy
cattle;
Contribution
=
(
tolerance
X
%
diet)
for
poultry
and
swine.
4
The
tolerance
value
for
pineapple
was
used
for
residues
in
pineapple
processing
waste.
5
MTDB
for
beef/
dairy
cattle
respectively.

In
an
adequate
ruminant
feeding
study,
three
groups
of
dairy
cattle
(
3
cows/
group)
were
dosed
orally
via
capsules
once
a
day
for
28­
30
consecutive
days
with
ametryn
(
97.2%
ai)
at
levels
equivalent
to
2.15,
6.20,
and
20.1
ppm
in
the
feed.
These
dose
levels
are
equivalent
to
12x,
34x
and
112x
the
MTBD
for
dairy
cattle.
Milk
samples
were
collected
on
Study
Days
1,
3,
7,
14,
21,
and
26,
and
samples
of
blood,
liver,
kidneys,
muscle,
and
fat
were
collected
within
24
hours
of
the
final
dose.
All
samples
were
stored
frozen
(
ca.
­
20

C)
for
up
~
12
months
prior
to
analysis,
an
interval
that
is
supported
by
the
available
storage
stability
data.

Milk
and
tissue
samples
were
analyzed
for
residues
of
ametryn
and
its
three
thiomethyl
metabolites
(
GS­
11354,
GS­
11355,
and
GS­
26831)
using
an
adequate
GC/
NPD
method
(
AG­
649).
The
validated
LOQ
for
ametryn
and
each
metabolite
is
0.02
ppm
in
tissues
and
0.01
ppm
in
milk,
for
combined
LOQs
of
0.08
and
0.04
ppm,
respectively.

All
samples
of
milk
and
tissues
were
analyzed
from
the
20.1
ppm
dose
group
(
112x),
but
only
milk
(
day
26)
and
muscle
were
analyzed
from
the
2.15
and
6.2
ppm
dose
groups
(
12x
and
34x).
Residues
of
each
analyte
were
<
0.01
ppm
(<
LOQ)
in
all
milk
samples
from
cows
dosed
at
20.1
ppm,
for
combined
residues
of
<
0.04
ppm,
and
residues
were
also
<
0.01
ppm
for
each
analyte
in
the
selected
milk
samples
from
the
2.15
and
6.2
ppm
dose
groups.
In
tissues,
residues
of
each
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
16
analyte
were
<
0.02
ppm
(<
LOQ)
in
blood,
liver,
kidney,
muscle,
and
fat
from
each
cow
in
the
20.1
ppm
dose
group,
for
combined
residues
of
<
0.08
ppm
in
each
matrix.

Based
on
the
results
of
the
ruminant
metabolism
and
feeding
study,
HED
concludes
that
there
is
no
reasonable
expectation
of
finite
residues
occurring
in
milk
and
tissues
of
cattle,
goats,
hogs,
horses,
and
sheep
[
40
CFR
§
180.6(
a)(
3)].
Therefore,
tolerances
for
ametryn
residues
in
livestock
commodities
are
not
required
to
support
the
current
use
pattern.

In
the
poultry
feeding
study,
three
groups
of
laying
hens
(
15
hens/
group)
were
fed
ad
libitum
for
28
consecutive
days
with
feed
containing
ametryn
at
nominal
levels
of
0.5,
1.5,
and
5.0
ppm,
which
are
equivalent
to
12.5x,
38x,
and
125x
the
MTDB.
Egg
samples
were
collected
on
Study
Days
0
(
pre­
dose),
1,
3,
7,
14,
21,
and
28
and
composited
by
subgroup.
Hens
were
sacrificed
44­
48
hours
following
removal
from
treated
feed
and
samples
of
liver,
muscle
(
composite
of
breast
and
leg),
skin
(
including
attached
fat)
and
fat
(
peritoneal)
were
collected
and
composited
by
subgroup.
All
samples
were
stored
frozen
until
analysis.
The
maximum
frozen
(
ca.
­
15

C)
storage
interval
was
20
months
for
eggs
and
tissues
and
is
not
supported
by
the
available
storage
stability
data,
which
assessed
residue
stability
up
to
only
14
months.
However,
because
the
poultry
dietary
burden
is
so
low,
HED
will
not
require
the
registrant
to
submit
addition
storage
stability
data
to
cover
the
extra
six
months
of
storage
time.
Samples
were
analyzed
for
residues
of
ametryn
and
its
thiomethyl
metabolites
using
an
adequate
GC/
NPD
method
(
AG­
649),
which
has
a
validated
LOQ
of
0.02
ppm
for
each
analyte
in
eggs
and
tissues.

All
collected
egg
and
tissue
samples
from
the
5
ppm
dose
group
were
analyzed,
but
only
liver,
muscle,
and
Day
7
egg
samples
were
analyzed
from
the
0.5
and
1.5
ppm
dose
groups
because
residues
in
the
high
dose
group
were
<
LOQ.
Residues
of
ametryn
and
each
metabolites
were
<
0.02
ppm
(<
LOQ)
in
all
analyzed
samples
of
whole
eggs
from
each
group,
for
combined
residues
of
<
0.08
ppm.
Residues
of
ametryn
and
each
metabolite
were
also
<
0.02
ppm
(<
LOQ),
in
all
tissues
samples
from
the
5
ppm
dose
group,
and
in
the
analyzed
muscle
and
liver
samples
from
the
0.5
and
1.5
ppm
dose
groups.
Combined
residues
were
<
0.08
ppm
in
each
tissue
sample.

Information
from
the
feeding
study
together
with
the
adequate
poultry
metabolism
study
are
sufficient
to
assess
the
need
for
tolerances
in
poultry
commodities
based
on
the
current
use
pattern.

The
available
residue
data
on
eggs
from
the
feeding
study
indicate
that
quantifiable
residues
are
unlikely
to
occur
in
eggs
as
residues
of
ametryn
and
the
thiomethyl
metabolites
were
each
<
LOQ
for
the
at
5
ppm
dose
group
(
125x).
The
egg
samples
were
collected
at
the
appropriate
intervals,
and
although
samples
were
stored
frozen
for
19­
20
months,
the
available
storage
stability
data
indicate
that
residues
in
frozen
eggs
are
completely
stable
for
at
least
14
months,
with
recoveries
of
>
100%.
Therefore,
substantial
declines
would
not
be
expected
by
20
months.

In
addition,
in
the
poultry
metabolism
study
conducted
for
3
days
at
1,250x
the
MTDB,
the
combined
residues
of
ametryn
and
its
three
thiomethyl
metabolites
in
eggs
and
tissues
ranged
from
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
17
0.03
ppm
in
yolks
to
0.42
ppm
in
liver.
If
residues
are
adjusted
to
a
100x
dose
level,
then
the
range
of
expected
combined
residues
would
be
0.002­
0.033
ppm,
which
are
below
the
combined
LOQ
of
0.08
ppm
for
the
analytical
method.
Considering
the
current
dietary
exposure
of
poultry
and
the
above
data,
HED
concludes
that
there
is
no
reasonable
expectation
of
finite
residues
occurring
in
poultry
commodities
[
40
CFR
§
180.6(
a)(
3)],
and
an
additional
poultry
feeding
study
is
not
required
are
the
present
time.

860.1500
Crop
Field
Trials
The
reregistration
requirements
for
magnitude
of
the
residue
in
plants
are
fulfilled
for
field
corn,
pineapple,
and
sugarcane.
An
adequate
number
of
tests
were
conducted
on
these
crops
in
the
appropriate
geographical
regions
at
the
maximum
labeled
use
rates.
Results
from
these
field
trials
are
summarized
below.
Field
trial
data
are
also
available
on
bananas;
however,
uses
on
bananas
and
plantains
have
been
deleted
from
the
most
recent
version
of
the
Syngenta's
label
for
the
80%
DF
(
EPA
Reg
No.
100­
786,
dated
3/
30/
04).
The
available
data
for
field
corn
will
also
support
the
use
on
pop
corn,
and
the
field
corn
forage
and
stover
data
can
be
used
to
cover
residues
in/
on
sweet
corn
forage
and
stover.
However,
data
are
required
depicting
residues
of
ametryn
and
its
three
thiomethyl
metabolites
in/
on
sweet
corn
ears
(
kernels
plus
cob
with
husks
removed)
if
the
use
on
sweet
corn
is
to
be
maintained.
Although
Syngenta
indicated
in
1999
that
the
use
on
sweet
corn
was
being
deleted
from
its
label
(
MRID
44783700),
the
most
recent
label
for
the
80%
DF
still
includes
use
directions
for
sweet
corn.

Field
Corn.
Adequate
data
are
available
from
a
total
of
28
tests
conducted
on
field
corn
throughout
the
U.
S.
Ametryn
(
80%
WP)
was
applied
using
ground
equipment
as
a
single
postemergence
directed
spray
at
2
lb
ai/
A
(
1x)
in
15
tests,
4
lb
ai/
A
(
2x)
in
9
tests,
6
lb
ai/
A
(
3x)
in
2
tests,
and
10
lb
ai/
A
(
5x)
in
2
tests.
Samples
of
forage
and
silage­
stage
forage
were
collected
from
each
test
at
28­
36
DAT
and
42­
85
DAT,
respectively;
and
samples
of
grain
and
fodder
were
collected
at
maturity
(
80­
126
DAT).
The
label
currently
specifies
30­
day
PHI
for
forage
and
prohibits
applications
within
3
weeks
of
tasseling.

For
the
tests
conducted
at
1x,
residues
of
ametryn
were
<
0.02­
0.10
ppm
in/
on
forage
(
n=
30),
<
0.02­
0.08
ppm
in/
on
silage­
stage
forage
(
n=
30),
and
<
0.02
ppm
in/
on
fodder
(
n=
30).
Residues
of
GS­
11354
were
<
0.02­
0.04,
<
0.02­
0.05,
and
<
0.02­
0.03
ppm
in
the
1x
forage,
silage­
stage
forage,
and
fodder
samples,
respectively.
Residues
of
GS­
11355
were
<
0.02­
0.04
ppm
in
the
1x
forage,
silage­
stage
forage,
and
fodder
samples.
Residues
of
GS­
26831
were
<
0.02­
0.02
ppm
in
the
1x
forage
samples,
and
were
<
0.02
ppm
in
the
1x
silage­
stage
forage
and
fodder
samples.
Maximum
combined
residues
were
0.20
ppm
in/
on
forage
and
<
0.11
ppm
in/
on
fodder.
Residues
of
ametryn,
GS­
11354,
and
GS­
26831
were
each
<
0.02
ppm
in
all
1x
treated
grain
samples
(
n=
29).
Residues
of
GS­
11355
were
also
<
0.02
ppm
in
all
treated
samples
except
in
two
samples
from
one
test
with
residues
at
0.03
and
0.04
ppm.

For
the
exaggerated
rate
tests,
residue
analyses
were
performed
on
a
total
of
51
samples
of
forage,
silage­
stage
forage,
and
fodder
treated
at
2x
(
9
samples
per
matrix),
3x
(
4
samples
per
matrix),
and
5x
(
4
samples
per
matrix).
Residues
were

0.16
ppm
for
ametryn
and
residues
of
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
18
GS­
11354,
GS­
11355,
GS­
26831
were
each
<
0.02­
0.04
ppm
in/
on
forage,
silage­
stage
forage,
and
fodder
samples
from
the
2x,
3x,
and
5x
tests.
Residues
of
ametryn
and
each
metabolite
were
<
0.02
ppm
in/
on
all
grains
samples
from
the
exaggerated
rate
trials
(
2x,
9
samples;
3x,
4
samples;
and
5x,
4
samples).

Pineapple.
Adequate
residue
data
are
available
from
a
total
of
13
tests
conducted
in
HI
on
pineapple.
Ametryn
(
80%
WP)
was
applied
as
a
single
broadcast
application
to
the
planting
or
ratoon
pineapple
crop
at
rates
of
7.2
lb
ai/
A
(
1x)
in
8
tests,
14.4
lb
ai/
A
(
2x)
in
3
tests,
and
21.6
lb
ai/
A
(
3x)
in
2
tests.
Samples
of
pineapple
were
harvested
at
142­
161
days
after
treatment;
the
labeled
PHI
is
160
days.
Residues
of
ametryn
were
<
0.02­
0.05
ppm
and
residues
of
GS­
11354,
GS­
11355,
and
GS­
26831
were
each
<
0.02
ppm
in/
on
16
samples
of
pineapples
from
the
1x
application;
combined
residues
were
<
0.08­<
0.11
ppm.
Residues
of
ametryn
and
each
metabolite
were
<
0.02
ppm
in/
on
the
6
treated
samples
from
the
2x
trials
and
the
4
treated
samples
from
the
3x
trials.

Sugarcane.
Residue
data
reflecting
a
variety
of
application
rates
and
timings
for
ametryn
(
80%
WP)
are
available
from
17
tests
conducted
on
sugarcane.
In
nine
of
the
tests
conducted
in
FL(
1),
HI(
4),
LA(
2),
and
PR(
2),
ametryn
was
applied
as
a
combination
of
one
preemergence
broadcast
application
at
5.6
lb
ai/
A
and
two
postemergence
broadcast
applications
at
2.4
and
4
lb
ai/
A
for
a
total
of
12
lb
ai/
A/
crop.
In
two
other
tests
conducted
in
FL,
ametryn
was
applied
as
three
directed
postemergence
applications
at
1.2
lb
ai/
A/
application
for
a
total
of
3.6
lb
ai/
A/
crop.
In
three
more
tests
conducted
in
TX,
ametryn
was
applied
as
one
preemergence
and
one
postemergence
broadcast
application
at
2.4
lb
ai/
A/
application
for
a
total
of
4.8
lb
ai/
A/
crop.
In
two
other
tests
in
LA,
ametryn
was
applied
as
one
broadcast
and
one
directed
postemergence
application
at
2.5
lb
ai/
A/
application
for
a
total
of
5
lb
ai/
A/
crop.
In
one
exaggerated
rate
test
in
HI,
ametryn
was
applied
as
three
broadcast
applications,
one
preemergence
and
two
postemergence
at
11.2,
4.8,
and
8
lb
ai/
A,
for
a
total
of
24
lb
ai/
A/
crop.
In
each
test,
the
final
postemergence
application
was
made
prior
to
close­
in.

Samples
of
mature
sugarcane
were
harvested
143­
300
days
following
the
final
treatment.
Residues
of
ametryn,
GS­
11354,
GS­
11355,
and
GS­
26831
were
each
<
0.02
ppm
in
all
33
treated
samples
of
sugarcane
(
without
leaves),
for
combined
residues
of
<
0.08
ppm.

The
current
use
directions
for
ametryn
on
sugarcane
generally
allow
for
a
preemergence
and
two
postemergence
broadcast
or
directed
applications,
with
the
last
application
being
made
prior
to
close­
in.
Use
rates
vary
considerably
depending
on
location.
Maximum
rates
allowed
per
sugarcane
crop
are
as
follows:
FL
­
3.6
lb
ai/
A;
HI
­
12
lb
ai/
A;
LA
­
6.8
lb
ai/
A;
PR
­
16
lb
ai/
A;
and
TX
­
6
lb
ai/
A.
Provided
the
maximum
use
rate
per
crop
cycle
is
restricted
to
a
maximum
of
12
lb
ai/
A
at
all
locations,
then
the
available
residue
data
for
sugarcane
are
adequate.
Syngenta
previously
indicated
in
a
letter
(
MRID
44783700)
that
the
maximum
use
rate
in
PR
would
be
reduced
to
12
lb
ai/
A;
however,
the
current
label
still
bears
the
higher
use
rate
(
16
lb
ai/
A)
for
PR.

Banana.
Although
Syngenta
is
no
longer
supporting
the
use
of
ametryn
on
bananas
and
plantains,
adequate
residue
data
are
available
from
8
tests
conducted
in
HI(
4)
and
PR(
4).
In
each
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
19
test,
ametryn
(
80%
WP)
was
applied
to
bananas
as
three
soil­
directed
applications
at

30­
day
intervals
using
ground
equipment.
Ametryn
was
applied
at
3.2
lb
ai/
A/
application
in
six
of
the
tests,
for
a
total
of
9.6
lb
ai/
A/
crop,
and
at
6.4
lb
ai/
A/
application
in
the
other
two
tests,
for
a
total
of
19.2
lb
ai/
A.
Banana
samples
were
harvested
6­
7
days
following
the
last
application
in
each
test,
and
subsamples
were
separated
into
peel
and
pulp.

Following
three
applications
totaling
9.6
lb
ai/
A/
crop,
residues
in/
on
11
treated
whole
banana
samples
were
<
0.02­
0.04
ppm
for
ametryn
and
<
0.02
ppm
each
for
GS­
11354,
GS­
11355,
and
GS­
26831,
for
combined
residues
of
<
0.08­<
0.12
ppm.
Residues
of
ametryn
and
the
three
metabolites
were
each
<
0.02
ppm
in
11
banana
pulp
samples,
for
combined
residues
of
<
0.08
ppm.
Following
three
applications
totaling
19.2
lb
ai/
A/
crop,
residues
in/
on
3
treated
whole
banana
samples
were
<
0.02­
0.17
ppm
for
ametryn,
<
0.02­
0.04
ppm
for
GS­
11354,
and
<
0.02
ppm
each
for
GS­
11355
and
GS­
26831,
for
combined
residues
of
<
0.08­<
0.25
ppm.
In
the
3
associated
pulp
samples,
residues
were
<
0.02
ppm
each
for
ametryn
and
the
three
metabolites,
for
combined
residues
of
<
0.08
ppm.

The
available
banana
residue
data
would
support
the
use
of
up
to
three
directed
applications
of
ametryn
(
WP,
FLC
or
DF)
to
bananas
or
plantains
at
3.2
lb
ai/
A/
application
at
30­
day
intervals,
using
ground
equipment,
for
a
maximum
use
rate
of
9.6
lb
ai/
A/
crop
cycle
with
a
7­
day
PHI.

860.1520
Processed
Food
and
Feed
The
reregistration
requirements
for
magnitude
of
the
residue
in
processed
food/
feed
are
satisfied
for
corn
and
pineapple,
but
not
sugarcane.
The
available
processing
data
for
corn
(
5x
rate)
and
pineapple
(
3x
rate)
indicate
that
residues
of
ametryn
and
its
three
thiomethyl
metabolites
are
not
likely
to
be
quantifiable
(

0.02
ppm)
in
corn
and
pineapple
processed
fractions
derived
from
crops
treated
at
the
maximum
labeled
rates.
However,
the
available
sugarcane
processing
study
is
not
adequate
because
residues
were
<
LOQ
in
cane
(
RAC)
following
applications
at
only
1x
the
maximum
rate.

Corn.
In
three
tests
conducted
in
IL,
ametryn
(
80%
WP)
was
applied
to
field
corn
as
a
single
postemergence,
directed
spray
at
either
2
lb
ai/
A
(
1x),
6
lb
ai/
A
(
3x),
or
10
lb
ai/
A
(
5x).
Bulk
corn
grain
samples
was
harvested
from
each
test
at
maturity,
116
days
post­
treatment,
and
processed
using
simulated
dry­
and
wet­
milling
procedures
into
the
following
fractions:
large
grits,
small
grits,
meal,
flour,
starch,
crude
oil
and
refined
oil.

Residues
of
ametryn,
GS­
11354,
and
GS­
26831
were
each
<
0.02
ppm
in
all
samples
of
corn
grain
and
processed
fractions
from
each
test.
Residues
of
GS­
11355
were
also
<
0.02
ppm
in
all
processed
fractions
with
the
exception
of
small
grits
(
0.02
ppm
in
the
3x
and
5x
samples)
and
wet­
milled
crude
oil
(
0.02
ppm
in
the
3x
and
5x
samples)
and
refined
oil
(
0.03
ppm
in
the
1x
sample).
However,
the
detectable
residues
were
reported
to
be
the
result
of
an
interfering
peak,
and
reanalysis
of
small
grits
and
crude
oil
using
GC/
MS
indicated
that
residues
of
ametryn
and
the
three
thiomethyl
metabolites
were
each
<
0.01
ppm
in
these
fractions.
As
residues
of
each
analyte
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
20
were
<
LOQ
in
all
processed
fractions
and
corn
grain
from
plants
treated
at
5x
the
maximum
label
rate,
no
additional
corn
grain
processing
data
are
required.

Pineapple.
In
four
tests
conducted
in
HI,
ametryn
(
80%
WP)
was
applied
to
pineapples
as
a
single
broadcast
application
at
either
7.2
lb
ai/
A
(
1x,
2
tests)
or
21.6
lb
ai/
A
(
3x,
2
tests).
Mature
pineapple
fruits
were
harvested
at
151­
161
days
post­
treatment
and
processed
using
simulated
commercial
procedures
into
bran,
pulp,
slices,
and
juice.

Residues
of
ametryn
and
the
three
metabolites
were
each
<
0.02
ppm
(<
LOQ)
in/
on
whole
pineapples
treated
at
1x
or
3x,
and
were
<
0.02
ppm
in
juice,
pulp,
and
slices,
and
<
0.1
ppm
(<
LOQ)
in
bran
processed
from
1x
and
3x
treated
fruits.
Although
pineapples
were
not
treated
at
the
maximum
theoretical
concentration
factor
of
3.8x,
HED
concludes
that
the
3x
treatment
rate
was
sufficient
considering
the
long
interval
between
application
and
harvest
of
fruit.
Therefore
the
pineapple
processing
study
is
adequate
and
indicates
that
residues
are
unlikely
to
concentrate
in
pineapple
processed
fractions.

Sugarcane.
In
two
tests
conducted
in
LA,
ametryn
(
80%
WP)
was
applied
to
sugarcane
as
either
one
broadcast
and
one
directed
postemergence
application
at
2.5
lb
ai/
A/
application
(
5
lb
ai/
A/
crop;
0.4x),
or
as
a
preemergence
broadcast
application
at
5.6
lb
ai/
A
and
two
postemergence
broadcast
applications
at
2.4
and
4
lb
ai/
A
(
12
lb
ai/
A/
crop;
1x).
Mature
canes
were
harvested
165
days
following
the
final
application
and
processed
using
simulated
commercial
procedures
into
bagasse,
cane
juice,
molasses,
raw
sugar,
and
refined
sugar.

Residues
of
ametryn,
GS­
11354,
GS­
11355,
and
GS­
26831
were
each
<
0.02
ppm
in
all
treated
fractions
(
1
sample
per
matrix
per
test),
except
that
residues
of
GS­
11355
were
0.02
ppm
in
the
treated
molasses
sample
from
the
0.4x
test.
The
sugarcane
processing
study
is
not
adequate
as
residues
were
<
LOQ
in
the
RAC
and
the
plants
were
only
treated
at
1x
the
maximum
rate.
Another
processing
study
should
be
conducted
using
sugarcane
bearing
detectable
residues
or
using
sugarcane
from
plants
treated
at
5x
the
maximum
label
rate.

860.1650
Submittal
of
Analytical
Reference
Standards
As
of
12/
10/
2003,
an
analytical
reference
standard
for
ametryn
is
available
at
the
EPA
National
Pesticide
Standards
Repository.

860.1850
Confined
Accumulation
in
Rotational
Crops
The
reregistration
requirement
for
confined
accumulation
in
rotational
crops
is
satisfied.
In
an
adequate
confined
study,
[
14C­
triazine]
ametryn
was
applied
three
times
to
greenhouse­
grown
sugarcane
plants
growing
in
a
sandy
loam
soil.
The
[
14C]
ametryn
was
applied
to
the
soil
as
a
preemergence
(
8
lb
ai/
A)
and
two
post­
emergence
soil­
directed
applications
(
4
lb
ai/
A/
application),
for
a
total
of
16
lb
ai/
A,
which
is
1x
the
maximum
seasonal
use
on
sugarcane
grown
in
Puerto
Rico
and
is
the
highest
rate
on
any
rotated
crop.
The
sugarcane
plants
were
harvest
152
days
after
the
final
treatment
(
DAT),
and
initial
attempts
at
planting
rotational
crops
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
21
at
153
and
259
DAT
were
unsuccessful
due
to
phytotoxicity.
Rotational
crops
of
lettuce,
soybean,
wheat
and
sugar
beets
were
successfully
planted
at
422
DAT
and
harvested
at
normal
crop
maturity.

Total
radioactive
residues
were
0.42
ppm
in
mature
lettuce
and
0.79
ppm
in
mature
sugar
beet
tops
and
roots.
In
wheat,
TRR
were
1.15­
1.37
ppm
in
forage,
6.56
ppm
in
straw,
and
1.08
ppm
in
grain.
In
soybeans,
TRR
were
0.41­
2.11
in
forage,
9.09
ppm
in
straw,
and
0.71
ppm
in
seeds.

Initial
solvent
extraction
released
64­
97%
of
the
TRR
from
all
samples,
and
a
total
of
86­
98%
of
the
TRR
was
extracted
following
acid
hydrolysis
of
residual
solids.
For
the
mature
matrices,
62.4­
84.4%
of
the
TRR
were
identified.
Although
there
were
quantitative
differences
in
metabolite
levels
between
matrices,
the
metabolite
profile
was
qualitatively
similar
among
the
various
rotational
crops.
The
major
14C­
residues
were
comprised
of
the
hydroxylated
triazine
metabolites,
G­
35173
(
12.5­
40.7%
TRR),
GS­
17794
(
0.8­
30.8%
TRR),
and
G­
28521
(
4.5­
12.7%
TRR).
Other
triazine
containing
metabolites
identified
in
rotational
crops
included
GS­
11354
(
ND­
2.4%
TRR),
GS­
26831/
CGA­
236433
(
ND­
10.7%
TRR),
G­
34048
(
ND­
3.3%
TRR),
and
GS­
17791
(
ND­
1.5%
TRR).
Ametryn
was
detected
in
all
rotation
crop
matrices
from
the
422­
day
PBI
at
levels
of
0.011­
0.229
ppm,
accounting
for
1.1­
23.0%
of
the
TRR.
Analysis
of
solvent
extractable
soil
14C­
residues
also
detected
ametryn,
G­
34048,
GS­
11354,
GS­
11355,
and
GS­
17794
in
the
soil.

The
results
of
the
confined
rotational
crop
study
were
similar
to
the
metabolism
of
ametryn
in
primary
crops.
Ametryn
is
metabolized
in
soil
and
plants
by
N­
dealkylation
and
desulfation
(
oxidation/
hydroxylation)
to
a
variety
of
metabolites
containing
a
hydroxylated
triazine
ring.

860.1900
Field
Accumulation
in
Rotational
Crops
The
reregistration
requirements
for
field
accumulation
in
rotational
crops
are
satisfied
provided
the
requested
label
amendments
are
made.
Current
use
directions
for
corn
allow
for
rotation
to
small
grains
(
e.
g.
wheat)
the
fall
following
application
and
restrict
rotation
to
all
other
crops
until
the
following
year.
However,
draft
use
directions
submitted
by
Syngenta
(
3/
19/
99)
would
specify
minimum
plant­
back
intervals
(
PBI)
of
3
months
for
wheat,
10
months
for
soybeans,
spinach,
and
potatoes,
and
11
months
for
all
other
rotational
crops.

Adequate
residue
data
are
available
from
extensive
rotational
crop
field
trials
and
processing
studies
depicting
residues
of
ametryn
and
its
three
thiomethyl
metabolites
in
the
RACs
and
processed
commodities
of
soybeans,
sorghum,
cotton,
and
peanuts
planted

11
months
following
a
single
application
of
ametryn
(
WP)
to
field
corn
at
either
2,
4,
6
or
10
lb
ai/
A
(
1x,
2x,
3x,
and
5x).
The
data,
which
are
discussed
in
detail
below,
indicate
that
residues
of
ametryn
and
its
three
metabolites
are
each
<
0.02
ppm
(<
LOQ)
in
regulated
RACs
and
processed
fractions
from
rotational
cotton,
peanuts,
sorghum,
and
soybeans
planted

11
months
following
a
1x
application
of
ametryn
to
corn.
These
data
support
a
general
11­
month
PBI
for
all
crops
following
ametryn
treated
corn,
and
indicate
that
rotational
crop
tolerances
are
not
required.
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
22
To
support
reduced
PBIs
of
3
months
for
small
grains
and
10
months
for
potatoes,
spinach,
and
soybeans,
Syngenta
has
also
submitted
two
limited
rotational
crop
field
trials
(
discussed
below)
depicting
residues
of
ametryn
per
se
in
RACs
from
these
crops
planted
following
an
application
to
the
soil
at
1x
the
maximum
use
rate
for
corn.
The
residue
data
for
wheat,
spinach
and
potatoes
are
adequate
and
indicate
that
residues
of
ametryn
were
<
0.02
ppm
in
all
regulated
RACs
from
wheat,
spinach
and
potatoes
planted

3
or

10
months
following
a
1x
application
to
corn.
However,
the
soybean
residue
data
are
not
adequate
and
do
not
support
reducing
the
PBI
to
10
months
for
soybeans
as
only
two
samples
of
soybean
seeds
were
collected
from
one
site
and
samples
of
soybean
forage
and
hay
were
not
collected
from
either
site.

Considering
all
of
the
rotational
field
crop
data,
the
registrants
should
amend
EP
labels
to
specify
PBIs
of
3
months
for
small
grains,
10
months
for
spinach
and
potatoes,
and
11
months
for
all
other
crops
planted
in
rotation
with
corn.

HED
also
notes
that
no
rotational
crop
intervals
have
been
proposed
following
application(
s)
of
ametryn
to
sugarcane.
Although
sugarcane
grown
in
HI,
PR
and
FL
is
not
typically
rotated
with
other
crops,
sugarcane
grown
in
the
Mississippi
Delta
region
(
TX
and
LA)
is
typically
rotated
after
3
years
to
soybeans
or
corn.
Considering
the
maximum
use
rates
on
sugarcane
in
LA
and
TX
(
6­
6.8
lb
ai/
A/
crop
cycle)
and
the
available
residue
data
from
the
extensive
rotational
crop
field
trials
(
3x
and
5x
tests)
discussed
below,
rotational
crop
tolerances
will
not
be
required
provided
that
the
label
is
amended
to
restrict
rotation
to
soybean,
sorghum,
or
cotton
with
a
PBI
of
11
months
following
application
of
ametryn
to
sugarcane.
No
rotational
crop
restriction
is
needed
for
corn
since
it
is
labeled
as
a
primary
crop
for
ametryn
use.

Extensive
rotational
field
trials.
In
a
total
of
14
tests
conducted
in
the
major
cotton
growing
regions,
cotton
was
planted

11
months
following
a
single
application
of
ametryn
(
80%
WP)
to
a
primary
crop
of
corn
at
a
rate
of
2
lb
ai/
A
(
6
tests),
4
lb
ai/
A
(
4
tests),
6
lb
ai/
A
(
2
tests),
or
10
lb
ai/
A
(
2
tests).
Duplicate
samples
of
cottonseed
were
collected
122­
189
day
after
planting
(
DAP)
from
each
test.
Residues
of
ametryn
and
the
three
metabolites
were
each
<
0.02
ppm
in
all
28
treated
samples
of
cottonseed,
including
samples
from
the
5x
tests.
Bulk
samples
of
undelinted
cottonseeds
from
one
of
each
of
the
1x,
3x,
and
5x
tests
were
also
processed
into
delinted
seeds,
hulls,
kernels,
meal,
soapstock,
and
oil.
Residues
of
ametryn
and
the
three
metabolites
were
each
<
0.02
ppm
in
all
processed
fractions.

In
a
total
of
17
tests
conducted
in
the
major
peanut
growing
regions
of
the
U.
S.,
peanuts
were
planted

11
months
following
a
single
application
of
ametryn
(
80%
WP)
to
a
primary
crop
of
corn
at
a
rate
of
2
lb
ai/
A
(
7
tests),
4
lb
ai/
A
(
6
tests),
6
lb
ai/
A
(
2
tests),
or
10
lb
ai/
A
(
2
tests).
At
maturity
(
116­
150
DAP),
duplicate
samples
of
peanut
nutmeats
and
vines
were
collected
from
15
tests
and
duplicate
samples
of
peanut
hay
were
collected
from
17
tests.
Residues
of
ametryn
and
the
three
metabolites
were
each
<
0.02
ppm
in
all
30
samples
of
peanut
nutmeats
and
vines,
and
all
34
samples
of
hay,
including
samples
from
5x
tests.
Bulk
samples
of
peanuts
from
one
of
each
of
the
1x,
3x,
and
5x
tests
were
also
processed
into
hulls,
kernels,
expeller
presscake,
soapstock
and
oil.
Residues
of
ametryn
and
the
three
metabolites
were
each
<
0.02
ppm
in
all
processed
fractions.
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
23
In
a
total
of
21
tests
conducted
throughout
the
U.
S.,
sorghum
was
planted

11
months
following
a
single
application
of
ametryn
(
80%
WP)
to
a
primary
crop
of
corn
at
a
rate
of
2
lb
ai/
A
(
10
tests),
4
lb
ai/
A
(
7
tests),
6
lb
ai/
A
(
2
tests),
or
10
lb
ai/
A
(
2
tests).
For
each
test,
duplicate
forage
and
hay
samples
were
collected
at
56­
60
DAP;
duplicate
silage­
stage
forage
samples
were
collected
at
71­
116
DAP;
and
duplicate
grain
and
stover
samples
were
collected
at
113­
182
DAP.
Residues
of
ametryn
and
the
three
metabolites
were
each
<
0.02
ppm
in
all
42
samples
each
of
sorghum
forage,
silage­
stage
forage,
hay,
grain
and
stover,
including
samples
from
the
5x
tests.
Bulk
samples
of
grain
from
one
of
each
of
the
1x,
3x,
and
5x
tests
were
also
processed
by
dryand
wet­
milling
into
bran,
grits,
flour
and
germ.
Residues
of
ametryn
and
the
three
metabolites
were
each
<
0.02
ppm
in
all
processed
fractions.

In
a
total
of
18
tests
conducted
throughout
the
U.
S.,
soybeans
were
planted

11
months
following
a
single
application
of
ametryn
(
80%
WP)
to
a
primary
crop
of
corn
at
a
rate
of
2
lb
ai/
A
(
9
tests),
4
lb
ai/
A
(
5
tests),
6
lb
ai/
A
(
2
tests),
or
10
lb
ai/
A
(
2
tests).
For
each
test,
duplicate
forage
and
hay
samples
were
collected
at
59­
92
DAP,
and
duplicate
samples
of
seeds
were
collected
at
maturity
(
126­
198
DAP).
Residues
of
ametryn
and
the
three
metabolites
were
each
<
0.02
ppm
in
all
38
samples
of
seeds,
including
samples
from
the
5x
tests.
For
forage,
residues
of
all
three
metabolites
were
each
<
0.02
ppm
in
all
38
samples
of
forage
from
all
tests.
Residues
of
ametryn
were
also
<
0.02
ppm
in
36
of
the
forage
samples
from
all
of
the
1x,
2x,
3x
tests
and
one
of
the
5x
tests.
In
the
other
5x
test,
residues
of
ametryn
were
0.03
ppm
in
two
forage
samples.
For
soybean
hay,
residues
of
all
three
metabolites
were
each
<
0.02
ppm
in
all
hay
samples
(
n=
41),
with
the
exception
of
one
hay
samples
from
one
5x
tests
with
residues
of
GS­
11354
at
0.02
ppm.
Residues
of
ametryn
in
hay
were
<
0.02
ppm
in
20
out
of
21
samples
from
the
1x
tests,
with
one
sample
bearing
ametryn
residues
of
0.04­
0.07
ppm.
HED
previously
concluded
in
the
Ametryn
Update
that
the
detectable
residues
in
the
single
1x­
treated
hay
sample
were
artifactual.
For
the
2x
tests,
residues
of
ametryn
in
hay
were
<
0.02­
0.10
ppm,
with
detectable
residues
in
5
of
the
12
samples.
Residues
of
ametryn
in
hay
were
also
<
0.02­
0.07
ppm
in
3
of
the
4
samples
from
the
3x
tests
and
were
0.03­
0.11
ppm
in
all
4
samples
from
the
5x
tests.
Bulk
samples
of
soybean
seeds
from
one
of
each
of
the
1x,
3x,
and
5x
tests
were
also
processed
into
hulls,
meal,
soapstock,
and
oil.
Residues
of
ametryn
and
the
three
metabolites
were
each
<
0.02
ppm
in
all
processed
fractions,
except
for
residues
of
GS­
11355
were
detected
at
0.02­
0.03
ppm
in
hulls
from
the
3x
and
5x
tests.
However,
apparent
residues
of
GS­
11355
were
also
detected
at
0.02
ppm
in
the
control
samples
of
hulls.
Therefore,
the
low
level
detection
of
GS­
11355
in
the
treated
hull
samples
is
considered
an
artifact.

44783702.
mem.
wpd
Limited
rotational
field
trials.
In
limited
field
rotational
crop
trials
conducted
at
two
sites
(
NC
and
IL),
ametryn
(
80%
DF)
was
applied
to
the
bare
soil
at
2.0
lb
ai/
A
(
1x
the
maximum
rate
for
corn).
Winter
wheat
was
planted
at
3­
4
months
after
treatment,
and
soybeans,
spinach,
and
potatoes
were
planted
8­
10
months
after
treatment.
With
the
exception
of
soybeans,
duplicate
samples
of
each
of
the
representative
RAC
commodities
were
harvested
at
intervals
reflecting
normal
agricultural
practices.
Wheat
forage
was
harvested
at
60­
228
DAP,
wheat
hay
was
harvested
186­
265
DAP,
wheat
grain
and
straw
were
harvested
219­
301
DAP,
potato
tubers
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
24
were
harvested
94­
139
DAP,
and
spinach
leaves
were
harvested
58­
84
DAP.
Samples
of
soybean
forage
and
hay
were
not
collected
from
either
trial
and
soybean
seeds
were
harvested
(
160
DAP)
from
only
one
of
the
two
sites.
All
samples
were
analyzed
for
residues
of
ametryn,
but
residues
of
the
three
thiomethyl
metabolites
(
GS­
11354,
GS­
11355
and
GS­
26831)
were
not
determined.
At
both
test
sites,
residues
of
ametryn
were
<
0.02
ppm
in
all
samples
of
rotational
wheat
(
forage,
hay,
straw,
and
grain)
from
the
3­
or
4­
month
PBI
and
in
spinach
and
potatoes
from
the
8­
or
10­
month
PBIs.
Residues
were
also
<
0.02
ppm
in
soybean
seeds
from
the
10­
month
PBI
at
one
site.
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
25
Table
5.
Residue
Chemistry
Science
Assessment
for
Reregistration
of
Ametryn
(
PC
Code
080801).

GLN
Data
Requirements
Current
Tolerances
(
ppm)
[
§
180.258]
Additional
Data
Needed?
MRID
Nos.
1
860.1200:
Directions
for
Use
N/
A
=
Not
Applicable
Yes
See
Table
3
and
Appendix
1
860.1300:
Nature
of
the
Residue
­
Plants
N/
A
No
41662301
41662302
441079012
860.1300:
Nature
of
the
Residue
­
Animals
N/
A
No
41662303
41662304
41662305
41662306
439310012
860.1340:
Residue
Analytical
Method
­
Plant
Commodities
N/
A
No
3
41557102
41557103
41872304
42391601
­
Livestock
commodities
N/
A
No
444777012
444777022
860.1360:
Multiresidue
Method
N/
A
No
413972014
414234014
860.1380:
Storage
Stability
Data
­
Plant
Commodities
N/
A
No
41557101
43335902
43342401
447837012
­
Livestock
commodities
N/
A
No
5
444777042
860.1400:
Magnitude
of
the
Residue
­
Water,
Fish,
and
Irrigated
Crops
N/
A
No
860.1460:
Magnitude
of
the
Residue
­
Food
Handling
N/
A
No
860.1480:
Magnitude
of
the
Residue
­
Meat,
Milk,
Poultry,
Eggs
­
Fat,
Meat,
and
Meat
Byproducts
of
Cattle,
Goats,
Hogs,
Horses,
and
Sheep
None
established
No
444777062
­
Milk
­
Eggs
and
the
Fat,
Meat,
and
Meat
Byproducts
of
Poultry
None
established
No6
444777052
860.1500:
Crop
Field
Trials
Root
and
Tuber
Vegetables
­
Cassava,
root
0.1
No7
­
Tanier
0.25
No7
­
Yam,
true,
tuber
0.25
No7
Cereal
Grains
­
Corn,
grain
0.25
No
41909501
­
Corn,
sweet,
kernel
plus
cob
with
husks
removed
0.25
Yes
8
Forage,
Fodder
and
Straw
of
Cereal
Grains
­
Corn,
forage
and
stover
0.5
No
41909501
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
Table
5.
Residue
Chemistry
Science
Assessment
for
Reregistration
of
Ametryn
(
PC
Code
080801).

GLN
Data
Requirements
Current
Tolerances
(
ppm)
[
§
180.258]
Additional
Data
Needed?
MRID
Nos.
1
26
Miscellaneous
Commodities
­
Banana
0.25
No9
41872303
­
Pineapple
0.25
No
41909502
­
Pineapple,
fodder
and
forage
0.25
No10
­
Sugarcane,
cane
0.25
No11
41846601
­
Sugarcane,
fodder
and
forage
0.25
No10
860.1520:
Processed
Food/
Feed
­
Corn,
grain
None
No
42391602
­
Pineapple
None
No
41909502
­
Sugarcane
None
Yes12
41846601
860.1650:
Submittal
of
Analytical
Reference
Standards
N/
A
No
860.1850:
Confined
Accumulation
in
Rotational
Crops
N/
A
No
41986301
41986302
860.1900:
Field
Accumulation
in
Rotational
Crops
N/
A
No
42391602
447837022
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
27
1.
Bolded
references
were
discussed
in
the
Ametryn
Phase
4
Review,
dated
11/
19/
90
from
S.
Funk.
Unless
otherwise
noted,
all
other
references
were
reviewed
or
discussed
in
conjunction
with
the
Ametryn
Reregistration
Update
(
12/
9/
94).

2.
DP
No.
D307104,
W.
Donovan,
03­
NOV­
2004
3.
Method
AG­
563
has
undergone
a
successful
independent
laboratory
method
validation
trial
and
has
been
validated
using
14C­
labeled
samples
from
the
corn
and
sugarcane
metabolism
studies.

4.
The
Ametryn
Update
indicated
that
Multiresidue
Method
Testing
studies
had
been
submitted
for
ametryn
and
its
thiomethyl
metabolites
and
hydroxy
triazine
metabolites,
but
did
not
summarize
the
findings
of
these
studies.

5.
The
available
storage
stability
data
for
livestock
commodities
are
from
a
14­
month
interim
report
of
a
study
designed
to
run
for
20
months.
In
order
to
cover
the
maximum
sample
storage
interval
for
eggs
from
the
poultry
feeding
study,
the
registrant
may
submit
the
final
report
detailing
the
stability
of
ametryn
and
its
metabolites
after
20
months
of
frozen
storage.

6.
Data
from
the
feeding
study
together
with
the
adequate
poultry
metabolism
study
are
sufficient
to
assess
the
need
for
tolerances
in
poultry
commodities.
Considering
the
current
dietary
exposure
of
poultry,
HED
concludes
that
there
is
no
reasonable
expectation
of
finite
residues
occurring
in
poultry
commodities
[
40
CFR
§
180.6(
a)(
3)].

7.
Syngenta
is
not
supporting
uses
on
cassava,
yams,
and
tanier,
and
there
is
no
indication
that
another
party
will
be
supporting
these
uses.
Therefore,
tolerances
on
these
commodities
should
be
revoked.

8.
Data
are
required
depicting
residues
of
ametryn
and
its
three
thiomethyl
metabolites
in/
on
sweet
corn
ears
(
kernels
plus
cob
with
husks
removed).
Alternatively,
the
use
on
sweet
corn
should
be
deleted
from
all
EPs.

9.
Syngenta
is
no
longer
supporting
the
use
of
ametryn
on
bananas
or
plantains
and
has
removed
these
uses
from
its
label
for
the
80%
DF.

10.
Forage
and
fodder
of
pineapple
and
sugarcane
are
no
longer
considered
significant
livestock
feed
items
and
are
therefore
not
regulated.

11.
The
available
sugarcane
residue
data
are
adequate
provided
the
maximum
use
rate
per
crop
cycle
is
restricted
to
a
maximum
of
12
lb
ai/
A
at
all
locations.

12.
The
sugarcane
processing
study
is
not
adequate.
Residues
were
<
LOQ
(<
0.02
ppm)
in/
on
sugarcane
(
RAC),
bagasse,
molasses,
and
sugar
derived
from
plants
treated
at
12
lb
ai/
A/
crop;
however,
this
rate
is
equivalent
to
only
1x
the
maximum
rate.
Another
processing
study
should
be
conducted
using
sugarcane
bearing
detectable
residues
or
using
sugarcane
from
plants
treated
at
up
to
5x
the
maximum
label
rate.
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
28
TOLERANCE
REASSESSMENT
SUMMARY
Tolerance
Reassessments
for
Ametryn
The
tolerances
listed
in
40
CFR
§
180.258(
a
and
c)
are
currently
expressed
in
terms
of
ametryn
(
2­
ethylamino)­
4­(
isopropylamino)­
6­(
methylthio)­
s­
triazine
per
se.
The
ametryn
risk
assessment
team
has
determined
that
the
residues
of
concern
for
tolerance
and
risk
assessment
purposes
consists
of
ametryn
per
se.
A
summary
of
ametryn
tolerance
reassessments
is
presented
in
Table
6.

Tolerances
Listed
Under
40
CFR
§
180.258
(
a
and
c):

Adequate
residue
data
are
available
to
reassess
the
established
tolerances
on
bananas,
corn
(
forage,
grain,
and
stover),
pineapples,
and
sugarcane.
However,
data
are
not
available
to
support
the
tolerance
on
sweet
corn,
kernels
plus
cobs
with
husks
removed.
If
no
registrant
intends
to
support
the
use
on
sweet
corn,
then
this
tolerance
should
be
revoked
once
use
directions
for
sweet
corn
are
deleted
from
all
EPs.

Although
Syngenta,
the
basic
producer,
has
deleted
uses
on
bananas
and
plantains
from
its
label,
adequate
residue
data
are
available
to
support
a
banana
tolerance
if
another
registrant
intends
to
support
this
use.
However,
use
directions
for
bananas
and
plantains
must
be
amended
to
reflect
the
use
pattern
supported
by
the
available
residue
data.
In
accordance
with
§
180.1(
h),
the
tolerance
on
banana
will
cover
plantains.

The
available
residue
data
indicate
that
tolerances
can
be
lowered
for
all
commodities
(
see
Table
6).
Tolerances
on
corn
grain,
forage,
and
stover
should
be
split
to
include
field
and
pop
corn
(
e.
g.
Corn,
field,
grain
and
Corn,
pop,
grain)

Based
on
the
available
livestock
metabolism
and
feeding
studies,
there
is
no
reasonable
expectation
of
finite
residues
occurring
in
livestock
commodities.
Therefore,
tolerances
for
livestock
commodities
are
not
currently
required.

The
tolerances
on
forage
and
fodder
of
pineapples
and
sugarcane
should
be
revoked
as
these
commodities
are
no
longer
regulated,
and
the
tolerances
on
cassava,
tanier,
and
yams
should
be
revoked
as
uses
on
these
crops
are
not
being
supported.
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
29
Table
6.
Tolerance
Reassessment
Summary
for
Ametryn.

Commodity
Current
Tolerance
(
ppm)
Range
of
Residues
1
(
ppm)
Tolerance
Reassessment
1
(
ppm)
Comment/[
Correct
Commodity
Definition]

Tolerances
Listed
Under
40
CFR
§
180.258(
a):

Banana
0.25
<
0.02­
0.04
Revoke
Use
is
not
being
supported.

Corn,
forage
0.5
<
0.02­
0.10
0.1
Tolerances
for
forage,
grain
and
stover
should
each
be
split
into
separate
tolerances
for
Corn,
field
and
Corn,
pop.
Corn,
grain
0.25
<
0.02
0.05
2
Corn,
stover
0.5
<
0.02
0.05
2
Corn,
sweet,
kernel
plus
cob
with
husks
removed
0.25
No
data
TBD
3
Residue
data
remain
outstanding.
If
uses
on
sweet
corn
are
deleted
from
EPs,
then
this
tolerance
should
be
revoked.

Pineapple
0.25
<
0.02­
0.05
0.05
2
Pineapple,
fodder
0.25
not
applicable
Revoke
These
commodities
are
no
longer
regulated
livestock
feed
items
Pineapple,
forage
0.25
not
applicable
Sugarcane,
cane
0.25
<
0.02
0.05
2
Sugarcane,
fodder
0.25
not
applicable
Revoke
These
commodities
are
no
longer
regulated
livestock
feed
items
Sugarcane,
forage
0.25
not
applicable
Tanier
0.25
no
data
Revoke
Uses
are
not
being
support
on
these
crops.
Yam,
true,
tuber
0.25
no
data
Tolerances
Listed
under
40
CFR
180.258(
c):

Cassava,
root
0.1
no
data
Revoke
Use
is
not
being
supported
1
The
range
of
residues
for
ametryn
per
se.
2
The
LOQ
for
the
current
enforcement
method
is
0.05
ppm
for
ametryn
per
se.
3
TBD
=
to
be
determined.
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
30
Codex/
International
Harmonization
There
are
no
questions
with
respect
to
Codex/
U.
S.
tolerance
compatibility
as
there
are
no
Codex
MRLs
for
residues
of
ametryn.
There
are
also
no
Canadian
MRLs
for
ametryn;
however,
Mexico
has
established
MRLs
for
ametryn
(
ametrina)
on
several
crops.
A
comparison
of
the
Mexican
MRLs
and
the
corresponding
reassessed
U.
S.
tolerances
is
presented
in
Table
7.

Table
7.
Mexican
MRLs
and
applicable
U.
S.
tolerances
for
Ametryn.
Recommendations
are
based
on
conclusions
following
reassessment
of
U.
S.
tolerances.

Mexican
MRLs
Reassessed
U.
S.
Tolerance,
ppm
Recommendation
and
Comments
Commodity,
As
Defined
MRL
(
mg/
kg)

Corn
0.25
grain
and
stover
­
0.05
forage
­
0.1
The
available
residue
data
support
the
lower
tolerances
on
all
commodities.
Pineapple
0.25
0.05
Plantain
0.25
Revoke
Sugar
cane
0.25
0.05
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
31
BIBLIOGRAPHY
Study
Citations
41397201
Yokley,
R.
(
1990)
Determination
of
Ametryn,
Prometryn,
and
Metabolites
by
U.
S.
Food
and
Drug
Administration
Multiresidue
Method
Testing:
Lab
Project
Number:
ABR­
89064:
ABR­
77060:
102065.
Unpublished
study
prepared
by
Ciba­
Geigy
Corp.
78
p.

41423401
Williams,
R.
(
1990)
Multiresidue
Method
Testing
of
Atrazine,
Simazine,
and
their
Chloro­
and
Hydroxy­
traizine
Metabolites
in
Crops
and
Animal
Tissues:
Lab
Project
ID:
ABR­
89010.
Unpublished
study
prepared
by
Ciba­
Geigy
Corp.
130
p.

41557101
Beidler,
W.
(
1990)
Ametryn:
Storage
Stability
of
Ametryn
and
Selected
Metabolites
in
Crops
Under
Freezer
Storage
Conditions
(
Six
Month
Interim
Report):
Lab
Project
Number:
ABR­
90058.
Unpublished
study
prepared
by
Ciba­
Geigy
Corp.
94
p.

41557102
Vargo,
J.
(
1990)
Analytical
Method
for
the
Determination
of
Ametryn
and
Metabolites
GS­
11354,
GS­
11355,
and
GS­
26831
in
Crops
and
Crop
Fractions
by
Gas
Chromatography:
Lab
Project
No:
AG­
563.
Unpublished
study
prepared
by
Ciba­
Geigy
Corp.
57
p.

41557103
Morris,
A.;
Clayton,
F.
(
1990)
Method
Validation
Ruggedness
Trial
for
Ciba­
Geigy
Method
AG­
563,
Analytical
Method
for
the
Determination
of
Ametryn
and
Metabolites
GS­
26831
in
Crop
Fractions
by
Gas
Chromatography:
Final
Report:
Lab
Project
Number:
90­
0078
CGAG.
Unpublished
study
prepared
by
EN­
CAS
Analytical
Laboratories.
19
p.

41662301
Detra,
R.;
Chib,
J.
(
1990)
Ametryn:
Metabolism
of
Triazine
carbon­
14
Ametryn
in
Sugarcane:
Lab
Project
Number:
N/
0963/
1800.
Unpublished
study
prepared
by
Battelle
Columbus
Div.
94
p.

41662302
Detra,
R.;
Chib,
J.
(
1990)
Ametryn:
Metabolism
of
Triazine­
carbon­
14­
Ametryn
in
Corn:
Lab
Project
Number:
N/
0963/
1800.
Unpublished
study
prepared
by
Battelle.
130
p.

41662303
Liu,
D.
(
1990)
Ametryn:
Metabolism
of
carbon
14­
Ametryn
in
Laying
Hens:
Analysis
and
Quantitation
of
Metabolites
in
Eggs,
Edible
Tissues,
and
Excreta:
Lab
Project
Number:
RPT0024.
Unpublished
study
prepared
by
Xenobiotic
Laboratories,
Inc.
350
p.

41662304
Lin,
P.
(
1990)
Metabolism
Study
in
Laying
Hens
Feeding
carbon
14­
Ametryn:
Lab
Project
Number:
P01736.
Unpublished
study
prepared
by
Biological
Test
Center.
43
p.
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
32
41662305
Liu,
D.
(
1990)
Ametryn:
Metabolism
of
carbon
14­
Ametryn
in
Lactating
Goats:
Analysis
and
Quantitation
of
Metabolites
in
Milk,
Edible
Tissues,
and
Excreta:
Lab
Project
Number:
RPT0023.
Unpublished
study
prepared
by
Xenobiotic
Laboratories,
Inc.
142
p.

41662306
Lin,
P.
(
1990)
Metabolism
Study
in
Lactating
Goat
Feeding
carbon­
14­
Ametryn:
Lab
Project
Number:
P01734.
Unpublished
study
prepared
by
Biological
Test
Center.
53
p.

41846601
Selman,
F.
(
1991)
Ametryn­
Magnitude
of
Residues
in
Sugarcane
Forage
,
Stripped
Cane,
and
Stripped
Cane
Processed
Fractions
Resulting
from
Applications
of
Evik
80W:
Lab
Project
Number:
ABR­
91002.
Unpublished
study
prepared
by
Ciba­
Geigy
Corp.
417
p.

41872303
Selman,
F.
(
1991)
Ametryn­
Magnitude
of
Residues
in
Whole
Bananas,
Banana
Peel,
and
Banana
Pulp
Resulting
From
Application
of
Evik
80W:
Lab
Project
Number:
ABR­
91003.
Unpublished
study
prepared
by
Ciba­
Geigy
Corp.
159
p.

41872304
Vargo,
J.
(
1991)
Validation
of
Analytical
Method
AG­
563
for
the
Determination
of
Ametryn,
GS­
11354,
GS­
11355,
and
GS­
26831
in
Corn
and
Sugarcane:
Lab
Project
Number:
ABR­
90049.
Unpublished
Study
prepared
by
Ciba­
Geigy
Corp.
50
p.

41909501
Selman,
F.
(
1991)
Ametryn:
Magnitude
of
Residues
in
Field
Corn
Forage,
Fodder,
Grain,
and
Processed
Corn
Grain
Fractions
Resulting
From
Applications
of
EVIK
80W:
Lab
Project
Number:
ABR­
91001.
Unpublished
study
prepared
by
Ciba­
Geigy
Corporation.
507
p.

41909502
Wong,
L.
(
1991)
Ametryn:
Pineapple
Residue
Study:
Lab
Project
Number:
36­
5294:
PGA­
ES
91­
5294:
96­
20­
26.
Unpublished
study
prepared
by
Hawaiian
Sugar
Planters'
Association.
208
p.

41986301
Thede,
B.
(
1991)
Uptake
and
Metabolism
in
Greenhouse
Grown
Rotational
Crops
Grown
after
Triazine­
Carbon
14­
Ametryn­
Treated
Sugarcane:
Lab
Project
Number:
ABR­
91043.
Unpublished
study
prepared
by
Ciba­
Geigy
Corp.
114
p.

41986302
Kazee,
B.
(
1991)
Analytical
Report:
Uptake
and
Metabolism
of
in
Greenhouse
Grown
Rotational
Crops
Grown
after
Triazine­
Carbon­
14­
Treated
Sugarcane:
Lab
Project
Number:
N0963­
18R.
Unpublished
study
prepared
by
Battelle,
Columbus
Div.
63
p.

42391601
Vargo,
J.
(
1991)
Analytical
Method
for
the
Determination
of
Ametryn,
Prometryn
and
Metabolites
GS­
11354,
GS­
11355
and
GS­
26831
in
Corn
Soapstock
and
Cottonseed
Soapstock
by
Gas
Chromatography
including
Validation
Data:
Lab
Project
Number:
METHOD
AG­
581.
Unpublished
study
prepared
by
Ciba­
Geigy
Corp.
38
p.
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
33
42391602
Selman,
F.
(
1992)
Ametryn:
Magnitude
of
the
Residues
in
Field
Corn
Forage,
Silage­
Stage
Fodder,
Fodder,
Grain
and
Processed
Corn
Grain
Fractions
Resulting
from
Application
of
Evik
80W:
Amendment
1:
Lab
Project
Number
ABR­
91001.
Unpublished
study
prepared
by
Ciba­
Geigy
Corp.
in
coop
with
ABC
Labs,
Inc.;
EN­
CAS
Analytical
Labs;
Harris
Labs,
Inc.
and
others.
1335
p.

43335902
Eudy,
L.
(
1994)
Storage
Stability
of
Ametryn
and
Selected
Metabolites
in
Crops
Under
Freezer
Storage
Conditions:
Lab
Project
Number:
ABR­
94020:
102925:
248­
89.
Unpublished
study
prepared
by
Ciba­
Geigy
Corp.
159
p.

43342401
Ciba­
Geigy
Corp.
(
1994)
Sample
Storage
of
Greenhouse
Grown
Rotational
Crops
Grown
After
Triazine­(
carbon
14)­
Ametryn
Treated
Sugarcane:
Response
to
EPA
Review:
Lab
Project
Number:
N­
0963­
18R:
ABR­
91043.
Unpublished
study.
6
p.

43931001
Wu,
J.
(
1995)
Ametryn:
Storage
Stability
on
Ametryn:
Goat
and
Hen
Metabolism:
(
Supplement):
Lab
Project
Number:
RPT0023:
RPT0024:
88085.
Unpublished
study
prepared
by
Xenobiotic
Labs,
Inc.
27
p.

44107901
Thalacker,
F.;
Ash,
S.
(
1996)
(
Carbon
14)­
Ametryn:
Nature
of
the
Residue
in
Bananas:
Lab
Project
Number:
HWI
6117­
270:
318­
94:
94.255.
Unpublished
study
prepared
by
Hazleton
Wisconsin,
Inc.
141
p.

44477701
Lin,
K.
(
1997)
Determination
of
Residues
of
Ametryn,
GS­
11354,
GS­
11355,
and
GS­
26831
in
Animal
Tissue,
Milk,
and
Egg:
Lab
Project
Number:
AG­
649:
632­
95:
102925.
Unpublished
study
prepared
by
Novartis
Crop
Protection,
Inc.
55
p.
{
OPPTS
860.1340}

44477702
Lin,
K.
(
1997)
Validation
of
Analytical
Method
AG­
649
for
Determination
of
Ametryn,
GS­
11354,
GS­
11355,
and
GS­
26831
in
Animal
Tissues,
Milk,
and
Poultry
Eggs
by
Capillary
Gas
Chromatography:
Lab
Project
Number:
ABR­
97127:
631­
95:
ANPHI­
96003.
Unpublished
study
prepared
by
Novartis
Crop
Protection,
Inc.
133
p.
{
OPPTS
860.1340}

44477703
Hayworth,
C.
(
1997)
Stability
of
Ametryn
and
Selected
Metabolites
in
Processed
Fractions
Under
Freezer
Storage
Conditions:
6­
Month
Interim
Report:
Lab
Project
Number:
ABR­
97124:
213­
96:
102925.
Unpublished
study
prepared
by
Novartis
Crop
Protection,
Inc.
107
p.
{
OPPTS
860.1380}

44477704
Hayworth,
C.
(
1997)
Stability
of
Ametryn
and
Selected
Metabolites
in
Meat,
Milk,
and
Eggs
Under
Freezer
Storage
Conditions:
14­
Month
Interim
Report:
Lab
Project
Number:
ABR­
97126:
222­
96:
102925.
Unpublished
study
prepared
by
Novartis
Crop
Protection,
Inc.
98
p.
{
OPPTS
860.1380}
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
34
44477705
Hamilton,
L.
(
1997)
Ametryn­­
Magnitude
of
the
Residues
in
Meat
and
Eggs
Resulting
from
the
Feeding
of
Three
Levels
to
Poultry:
Lab
Project
Number:
ABR­
96110:
134­
95:
BIOL­
95005.
Unpublished
study
prepared
by
Novartis
Crop
Protection,
Inc.
187
p.
{
OPPTS
860.1480}

44477706
Boyette,
S.
(
1997)
Ametryn­­
Magnitude
of
the
Residues
in
Meat
and
Milk
Resulting
from
the
Feeding
of
Three
Levels
to
Dairy
Cattle:
Lab
Project
Number:
ABR­
96046:
144­
95:
BIOL­
95006.
Unpublished
study
prepared
by
Novartis
Crop
Protection,
Inc.
186
p.
{
OPPTS
860.1480}

44783701
Hayworth,
C.
(
1999)
Stability
of
Ametryn
and
Selected
Metabolites
in
Processed
Fractions
Under
Freezer
Storage
Conditions:
Final
Report:
Lab
Project
Number:
213­
96.
Unpublished
study
prepared
by
Novartis
Crop
Protection,
Inc.
100
p.
{
OPPTS
860.1380}

44783702
Lin,
K.
(
1999)
Ametryn­­
Field
Rotational
Crops
Following
Bare
Ground
Application
of
Evik
80W:
Final
Report:
Lab
Project
Number:
80­
95:
102925:
80­
95­
A.
Unpublished
study
prepared
by
Novartis
Crop
Protection,
Inc.
107
p.
{
OPPTS
860.1900}
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
35
Agency
Memoranda
Citations
Table
8.
Agency
Memoranda
Citations.

Date
DP
Barcode
CB
No.
From
To
MRID
Nos.
Subject
12/
9/
94
D165469,
D167228,

D167229,
D180728,

D182840,
D182842,

D190031,
D206668,

D206842
8140,
10230,

10617,
10618,

12934,
12935,

12936,
14228,

14259
R.
Perfetti
K.
Davis
41662301­
41662306,

41846601,
41872303,

41872304,
41909501,

41909502,
42391601,

42391602,
43342401,

43335902
Response
to
the
Ametryn
DCI:
Update
List
B
Case
No.
2010;
Chemical
No.
080801.

12/
22/
94
D209893
14812
R.
Perfetti
K.
Davis
None
Response
to
the
Ametryn
DCI:
List
B
Case
No.

2010;
Chemical
No.
080801.
Data
Waiver
Request
11/
3/
04
D309463
None
W.
Donovan
M.
Howard
44107901
DER:
Banana
Metabolism
Study
43931001
DER:
Supporting
Storage
Stability
Data
for
Goat
and
Poultry
Metabolism
Studies
44477701,
44477702
DER:
Residue
Analytical
Method
for
Livestock
commodities
44477704
DER:
Interim
Storage
Stability
Data
for
Residues
in
Livestock
commodities
44783701,
44477703
DER:
Storage
Stability
Data
for
Residues
in
Pineapples
and
Plant
Processed
Commodities
44477705
DER:
Poultry
Feeding
Study
44477706
DER:
Cattle
Feeding
Study
44783702
DER:
Field
Rotational
Crop
Data
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
36
List
of
Attachments
1.
Food
Use
Pattern
Table
for
Ametryn
2.
43931001.
der.
wpd
3.
44107901.
der.
wpd
4.
44477701.
der.
wpd
5.
44477704.
der.
wpd
6.
44477705.
der.
wpd
7.
44477706.
der.
wpd
8.
44783701.
der.
wpd
9.
44783702.
der.
wpd
cc
(
with
all
Attachments):
W.
Donovan
RDI:
HED
ChemSAC
(
22­
SEP­
2004),
D.
Drew
(
13­
OCT­
2004)
W.
H.
Donovan:
821G:
CM#
2:(
703)
305­
7330:
7509C:
RRB3
Ametryn
Residue
Chemistry
Considerations
­
RED
Barcode:
D307104
37
ATTACHMENT
1
Food/
Food
Use
Pattern
Table
for
Ametryn
Generated
by
BEAD/
OPP
TABLE
A2.
FOOD/
FEED
USE
PATTERNS
SUMMARY
FOR
Ametryn
(
ANSI)
(
CASE
2010)

Current
As
Of:
04/
02/
2003
Printed
On:
02/
10/
2004
Appendix
1
­
Page
1
SITE
NAME
LIMITATIONS
Application
Timing
(
for
any
Reg.#
at
any
rate)

Application
Type
(
for
any
Reg.#
at
any
rate)

Application
Equipment
(
for
any
Reg.#
at
any
rate)
Max.
Single
Appl.

Rate
to
a
Single
Site
Max.
Seasonal
Rate
Max.
#

Apps/

cc
&
yr
M
R
I
R
E
I
PHI/
PGI/
PSI
Use
Limitations
(
May
not
apply
to
all
Reg.
#
s)

BANANA
Do
not
apply
directly
to
water,
or
to
areas
where
surface
water
is
present
or
to
intertidal
areas
below
the
mean
high
water
mark.

Do
not
apply
through
any
type
of
irrigation
system.

Do
not
contaminate
water
by
cleaning
of
equipment
or
disposal
of
equipment
wash
waters.

Do
not
contaminate
water,
food,
or
feed
by
storage
or
disposal.

Do
not
enter
treated
areas
without
protective
clothing
until
sprays
have
dried.

Groundwater
restriction.

Rotational/
plant
back
crop
restriction.

Foliar
Basal
spray
treatment/
Broadcast/
Spray
Ground/
Sprayer
8
lb
A
24
lb/
yr
NS
NS
30
12
h
Geographic
allowable:
Other
Postplant
Basal
spray
treatment/
Spray
Ground/
Sprayer
8
lb
A
24
lb/
yr
NS
NS
90
12
h
Geographic
allowable:
HI
PR
CORN,
FIELD
30
day(
s)
prefeeding,
pregrazing
and
preharvest
intervals.

Do
not
apply
directly
to
water,
or
to
areas
where
surface
water
is
present
or
to
intertidal
areas
below
the
mean
high
water
mark.

Do
not
apply
through
any
type
of
irrigation
system.

Do
not
contaminate
water
by
cleaning
of
equipment
or
disposal
of
equipment
wash
waters.

Do
not
contaminate
water,
food,
or
feed
by
storage
or
disposal.

Do
not
enter
treated
areas
without
protective
clothing
until
sprays
have
dried.

Groundwater
restriction.

Rotational/
plant
back
crop
restriction.

Geographic
allowable:
AL
AR
AZ
CA
CO
CT
DE
FL
GA
IA
ID
IL
IN
KS
KY
LA
MA
MD
ME
MI
MN
MO
MS
MT
NC
ND
NE
NH
NJ
NM
NV
NY
OH
OK
OR
PA
RI
SC
SD
TN
TX
UT
VA
VT
WA
WI
WV
Foliar
Basal
spray
treatment/
Broadcast
Sprayer
2
lb
A
2
lb/
yr
NS
NS
NS
12
h
Postemergence
Directed
spray
Sprayer
2
lb
A
NS
NS
NS
NS
12
h
Geographic
allowable:
AK
HI
TABLE
A2.
FOOD/
FEED
USE
PATTERNS
SUMMARY
FOR
Ametryn
(
ANSI)
(
CASE
2010)

Current
As
Of:
04/
02/
2003
Printed
On:
02/
10/
2004
Appendix
1
­
Page
2
SITE
NAME
LIMITATIONS
Application
Timing
(
for
any
Reg.#
at
any
rate)

Application
Type
(
for
any
Reg.#
at
any
rate)

Application
Equipment
(
for
any
Reg.#
at
any
rate)
Max.
Single
Appl.

Rate
to
a
Single
Site
Max.
Seasonal
Rate
Max.
#

Apps/

cc
&
yr
M
R
I
R
E
I
PHI/
PGI/
PSI
Use
Limitations
(
May
not
apply
to
all
Reg.
#
s)

CORN,
POP
30
day(
s)
prefeeding,
pregrazing
and
preharvest
intervals.

Do
not
apply
directly
to
water,
or
to
areas
where
surface
water
is
present
or
to
intertidal
areas
below
the
mean
high
water
mark.

Do
not
apply
through
any
type
of
irrigation
system.

Do
not
contaminate
water
by
cleaning
of
equipment
or
disposal
of
equipment
wash
waters.

Do
not
contaminate
water,
food,
or
feed
by
storage
or
disposal.

Do
not
enter
treated
areas
without
protective
clothing
until
sprays
have
dried.

Groundwater
restriction.

Rotational/
plant
back
crop
restriction.

Geographic
allowable:
AL
AR
AZ
CA
CO
CT
DE
FL
GA
IA
ID
IL
IN
KS
KY
LA
MA
MD
ME
MI
MN
MO
MS
MT
NC
ND
NE
NH
NJ
NM
NV
NY
OH
OK
OR
PA
RI
SC
SD
TN
TX
UT
VA
VT
WA
WI
WV
Foliar
Basal
spray
treatment/
Broadcast
Sprayer
2
lb
A
2
lb/
yr
NS
NS
NS
12
h
Postemergence
Directed
spray
Sprayer
2
lb
A
NS
NS
NS
NS
12
h
Geographic
allowable:
AK
HI
CORN,
SWEET
30
day(
s)
prefeeding,
pregrazing
and
preharvest
intervals.

Do
not
apply
directly
to
water,
or
to
areas
where
surface
water
is
present
or
to
intertidal
areas
below
the
mean
high
water
mark.

Do
not
apply
through
any
type
of
irrigation
system.

Do
not
contaminate
water
by
cleaning
of
equipment
or
disposal
of
equipment
wash
waters.

Do
not
contaminate
water,
food,
or
feed
by
storage
or
disposal.

Do
not
enter
treated
areas
without
protective
clothing
until
sprays
have
dried.

Groundwater
restriction.

Rotational/
plant
back
crop
restriction.

Geographic
allowable:
AL
AR
AZ
CA
CO
CT
DE
FL
GA
IA
ID
IL
IN
KS
KY
LA
MA
MD
ME
MI
MN
MO
MS
MT
NC
ND
NE
NH
NJ
NM
NV
NY
OH
OK
OR
PA
RI
SC
SD
TN
TX
UT
VA
VT
WA
WI
WV
Foliar
Basal
spray
treatment/
Broadcast
Sprayer
2
lb
A
2
lb/
yr
NS
NS
NS
12
h
Postemergence
Directed
spray
2
lb
A
NS
NS
NS
NS
12
h
Geographic
allowable:
AK
HI
TABLE
A2.
FOOD/
FEED
USE
PATTERNS
SUMMARY
FOR
Ametryn
(
ANSI)
(
CASE
2010)

Current
As
Of:
04/
02/
2003
Printed
On:
02/
10/
2004
Appendix
1
­
Page
3
SITE
NAME
LIMITATIONS
Application
Timing
(
for
any
Reg.#
at
any
rate)

Application
Type
(
for
any
Reg.#
at
any
rate)

Application
Equipment
(
for
any
Reg.#
at
any
rate)
Max.
Single
Appl.

Rate
to
a
Single
Site
Max.
Seasonal
Rate
Max.
#

Apps/

cc
&
yr
M
R
I
R
E
I
PHI/
PGI/
PSI
Use
Limitations
(
May
not
apply
to
all
Reg.
#
s)

Sprayer
PINEAPPLE
160
day(
s)
preharvest
interval.

Do
not
apply
directly
to
water,
or
to
areas
where
surface
water
is
present
or
to
intertidal
areas
below
the
mean
high
water
mark.

Do
not
apply
through
any
type
of
irrigation
system.

Do
not
contaminate
water
by
cleaning
of
equipment
or
disposal
of
equipment
wash
waters.

Do
not
contaminate
water,
food,
or
feed
by
storage
or
disposal.

Do
not
enter
treated
areas
without
protective
clothing
until
sprays
have
dried.

Groundwater
restriction.

Rotational/
plant
back
crop
restriction.

Postharvest
Broadcast/
Spray
Sprayer
8
lb
A
30
lb/
cc
NS
NS
30
12
h
160
day(
s)
preharvest
interval.

Postplant
Broadcast/
Spray
Sprayer
8
lb
A
30
lb/
cc
NS
NS
30
12
h
PLANTAIN
Do
not
apply
directly
to
water,
or
to
areas
where
surface
water
is
present
or
to
intertidal
areas
below
the
mean
high
water
mark.

Do
not
apply
through
any
type
of
irrigation
system.

Do
not
contaminate
water
by
cleaning
of
equipment
or
disposal
of
equipment
wash
waters.

Do
not
contaminate
water,
food,
or
feed
by
storage
or
disposal.

Do
not
enter
treated
areas
without
protective
clothing
until
sprays
have
dried.

Groundwater
restriction.

Rotational/
plant
back
crop
restriction.

Foliar
Basal
spray
treatment/
Broadcast/
Spray
Ground/
Sprayer
8
lb
A
24
lb/
yr
NS
NS
30
12
h
Geographic
allowable:
Other
Postplant
Basal
spray
treatment/
Spray
Ground/
Sprayer
8
lb
A
24
lb/
yr
NS
NS
90
12
h
Geographic
allowable:
HI
PR
TABLE
A2.
FOOD/
FEED
USE
PATTERNS
SUMMARY
FOR
Ametryn
(
ANSI)
(
CASE
2010)

Current
As
Of:
04/
02/
2003
Printed
On:
02/
10/
2004
Appendix
1
­
Page
4
SITE
NAME
LIMITATIONS
Application
Timing
(
for
any
Reg.#
at
any
rate)

Application
Type
(
for
any
Reg.#
at
any
rate)

Application
Equipment
(
for
any
Reg.#
at
any
rate)
Max.
Single
Appl.

Rate
to
a
Single
Site
Max.
Seasonal
Rate
Max.
#

Apps/

cc
&
yr
M
R
I
R
E
I
PHI/
PGI/
PSI
Use
Limitations
(
May
not
apply
to
all
Reg.
#
s)

SUGARCANE
Do
not
apply
directly
to
water,
or
to
areas
where
surface
water
is
present
or
to
intertidal
areas
below
the
mean
high
water
mark.

Do
not
apply
through
any
type
of
irrigation
system.

Do
not
contaminate
water
by
cleaning
of
equipment
or
disposal
of
equipment
wash
waters.

Do
not
contaminate
water,
food,
or
feed
by
storage
or
disposal.

Do
not
enter
treated
areas
without
protective
clothing
until
sprays
have
dried.

Groundwater
restriction.

Rotational/
plant
back
crop
restriction.

Foliar
Band
treatment/
Basal
spray
treatment/
Broadcast/
Soil
band
treatment
Aircraft/
Band
sprayer/
Ground/
Sprayer
(
L)

4
lb
A
NS
3/
cc
NS
30
12
h
Geographic
allowable:
FL
LA
PR
Postemergence
Broadcast
Ground/
Sprayer
2
lb
A
NS
3/
cc
NS
NS
12
h
Geographic
allowable:
TX
Preemergence
Basal
spray
treatment/
Broadcast/
Soil
broadcast
treatment
Ground/
High
pressure
sprayer/
Sprayer
8
lb
A
16
lb/
cc
3/
cc
NS
30
12
h
Geographic
allowable:
HI
PR
TX
Ratoon
Band
treatment/
Basal
spray
treatment/
Broadcast/
Soil
band
treatment
Band
sprayer/
Ground/
Sprayer
(
L)

8
lb
A
NS
3/
cc
NS
30
12
h
Geographic
allowable:
FL
HI
LA
PR
Tops
Broadcast
Sprayer
2
lb
A
NS
NS
NS
NS
12
h
Geographic
allowable:
LA
When
needed
Broadcast
Aircraft
4
lb
A
NS
NS
NS
NS
12
h
PRODUCT
NUMBERS
CONTAINED
IN
THIS
REPORT
000100­
00786,
048273­
00003,
051036­
00105
HOMEOWNER
PRODUCTS
CONTAINED
IN
THIS
REPORT
None
TABLE
A2.
FOOD/
FEED
USE
PATTERNS
SUMMARY
FOR
Ametryn
(
ANSI)
(
CASE
2010)

Current
As
Of:
04/
02/
2003
Printed
On:
02/
10/
2004
Appendix
1
­
Page
5
SITE
NAME
LIMITATIONS
Application
Timing
(
for
any
Reg.#
at
any
rate)

Application
Type
(
for
any
Reg.#
at
any
rate)

Application
Equipment
(
for
any
Reg.#
at
any
rate)
Max.
Single
Appl.

Rate
to
a
Single
Site
Max.
Seasonal
Rate
Max.
#

Apps/

cc
&
yr
M
R
I
R
E
I
PHI/
PGI/
PSI
Use
Limitations
(
May
not
apply
to
all
Reg.
#
s)

HEADER
ABBREVIATIONS
Site
Name
­
The
site
name
refers
to
the
entity
(
crop,
building,
surface
or
article)
where
a
pesticide
is
applied
and/
or
which
is
being
protected.

Limitations
­
Precautionary
statements
related
to
the
use
of
the
product(
s).

Application
Timing
­
The
timing
of
pesticide
application
and
is
the
primary
application
sort
(
not
aggregated).

Application
Type
­
The
type
of
pesticide
application
(
aggregated).

Application
Equipment
­
The
equipment
used
to
apply
pesticide
(
aggregated).

Max.
Single
Appl.
Rate
to
a
Single
Site
­
Maximum
Dose
for
a
single
application
to
a
single
site.
System
calculated.

Max
Seasonal
Rate
­
The
maximum
amount
of
pesticide
that
can
be
applied
to
a
site
in
one
growing
season
(/
cc)
and
during
the
span
of
one
year
(/
yr).

Max.
#
Apps/
cc
&
yr
­
Maximum
Number
of
Applications
per
crop
cycle
and
per
year.

M
R
I
­
Minimum
Retreatment
Interval
(
days)
(
at
any
rate).
The
minimum
interval
between
pesticide
application
(
days).

R
E
I
­
ReEntry
Interval
­
The
minimum
amount
of
time
that
must
elapse
before
workers
can
reenter
a
treated
area.

PHI/
PGI/
PSI
Use
Limitations
(
May
not
apply
to
all
Reg.#
s)
­
Preharvest/
Pregrazing/
Preslaughter
Interval
use
limitations
pertinent
to
the
application.

Current
As
Of:
­
The
label
data
for
the
listed
products
in
this
report
is
current
of
this
date.

ABBREVIATIONS
AN
­
As
needed
NA
­
Not
Applicable
NS
­
Not
Specified
(
on
label)

(
L)
­
The
dosage
information
provided
is
from
the
label
in
terms
of
product
(
e.
g.,
ounces,
gallons,
or
pounds
of
the
product)
because
there
was
insufficient
information
(
e.
g.,
missing
density,
area,
or
active
ingredient
percentages)
to
provide
converted
dosage
information.
This
report
provides
active
ingredient
percentage
in
the
product
for
the
reported
chemical
for
all
unconverted
label
dosage
information
if
this
information
is
available.
This
active
ingredient
percentage
information
is
displayed
next
to
the
form
code
abbreviations
(
e.
g.,
80%
WP).

APPLICATION
RATE
cwt
:
Hundred
Weight
nnE­
xx
:
nn
times
(
10
power
­
xx),
for
instance,
"
1.234E­
04"
is
equivalent
to
".
0001234"