Document ID: EPA-HQ-OW-2002-0039-0086
Agency: epa
Document Type: Supporting & Related Material
Title: 
Posted Date: 2003-07-09T04:00Z

Draft
June
2003
Laboratory
name:
Laboratory
ID:

Method
1622/
1623
Slide
Examination
Form
Client
sample
number:
Internal
laboratory
sample
ID
(
if
applicable):

Analyst:
10­
mL
subsample
ID
(
if
packed
pellet
>
0.5
mL):

Examination/
confirmation
completion
date:
Volume
examined
(
in
L)
on
this
slide:
Exam./
confirm.
completion
time
:
(
must
be
completed
within
7
days
of
staining)
Positive
staining
control
acceptable
9
YES
9
NO
Negative
staining
control
acceptable
9
YES
9
NO
Cryptosporidium
Results
Object
located
by
FA
No.
Shape
(
oval
or
round)
Size
L
x
W
(
µ
m)
DAPI
­
DAPI
+
D.
I.
C.
Light
blue
internal
staining,
no
distinct
nuclei,
green
rim
(
A)
Intense
blue
internal
staining
(
B)
Number
of
nuclei
stained
sky
blue
(
C)
Empty
oocysts
(
D)
Oocysts
with
amorphous
structure
(
E)
Oocysts
with
internal
structure
(
F)

Number
of
sporozoites
1
2
3
4
5
6
7
8
9
10
Total
FA
number
from
this
slide:
D.
I.
C.
­
Total
number
of
empty
oocysts
(
D):

DAPI
­:
Total
number
(
A):
D.
I.
C.
­
Total
number
of
oocysts
with
amorphous
structure
(
E):

DAPI
+:
Total
number
(
B):
D.
I.
C.
­
Total
number
of
oocysts
with
internal
structure
(
F):

DAPI
+:
Total
number
(
C):
Total
count
DAPI
+
(
C)
that
show
structure
by
D.
I.
C.
(
F):

Giardia
Results
Object
located
by
FA
No.
Shape
(
oval
or
round)
Size
L
x
W
(
µ
m)
DAPI
­
DAPI
+
D.
I.
C.

Light
blue
internal
staining,
no
distinct
nuclei,
green
rim
(
A)
Intense
blue
internal
staining
(
B)
Number
of
nuclei
stained
sky
blue
(
C)
Empty
cysts
(
D)
Cysts
with
amorphous
structure
(
E)
Cysts
with
internal
structure
(
F)

Number
of
nuclei
Median
body
Axonemes
1
2
3
4
5
6
7
8
9
10
Total
FA
number
from
this
slide:
D.
I.
C.:
Total
number
of
empty
cysts
(
D):

DAPI­:
Total
number
(
A):
D.
I.
C.:
Total
number
of
cysts
with
amorphous
structure
(
E):
DAPI+:
Total
number
(
B):
D.
I.
C.:
Total
number
of
cysts
with
one
internal
structure
(
F):
DAPI+:
Total
number
(
C):
D.
I.
C.:
Total
number
of
cysts
with
>
one
internal
structure
(
F):

Total
number
DAPI
+
(
C)
that
show
structure
by
D.
I.
C.
(
F):