cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Targeting an audience: A discussion of relevant variables and a test of priming
Creators: name: Lane, Kaylin
name: Thomas, Hunter Scott
Publisher: name: Taylor & Francis
Year: 2022
Subjects: subject: Biochemistry
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Ecology
subject: FOS: Biological sciences
subject: Science Policy
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified

description: “Targeting an Audience: A Discussion of Relevant Variables and a Test of Priming” is a teaching activity created to further students’ knowledge of persuasive message reception and priming—a persuasion tactic often used in advertising by brands. Explanations of intended courses, learning objectives, and rationale are provided to help instructors understand why this activity may be impactful in their classrooms. Additionally, advice for facilitating the activity, including information on the materials, preparation, and a breakdown of the activity’s two parts, is provided as a guide for instructors to add this activity to their lesson plan. This activity was written for submission after successful facilitation in a graduate-level communication course, although applications extend beyond this level. An appraisal of the activity suggests limitations and variations of the activity. Originally developed as part of an instructional discussion for a graduate seminar in persuasion, “Targeting an Audience: A Discussion of Relevant Variables and a Test of Priming” is presented in this manuscript as an engaging activity to introduce any college-level student to the ideas of persuasive message reception and priming. Myers et al. (A curricular view of communication course offerings of national communication association department members. Communication Education, 70(4), 421–434, 2021) found persuasion to be the second most commonly offered course among National Communication Association department members, which may suggest that materials and activities to teach persuasion, such as presented in this manuscript, are in high demand. Courses: This activity is intended for use as part of a persuasive communication course lesson in which persuasive message effects on receivers are discussed. Although originally developed for a graduate seminar in persuasion, this activity may also be facilitated in undergraduate persuasion or introductory communication courses. Objectives: By participating in this activity, students will: (1) be able to define individual variables that influence a message receiver; (2) understand the effect of the variables on message reception through discussion of basic research findings and personal experiences; (3) apply the variables to suggest the “target audience” of real-life advertising examples; and (4) understand that receivers are not blank slates.
Application of Lightning Strike Protection on Thermoplastic Structures by Automated Fiber Placement
Creators: name: Nguyen, Chinh
name: Kolbe, Andreas
name: Bäns, Constantin
Publisher: name: Zenodo
Year: 2020
Subjects: Lightning strike protection, automated fiber placement

description: Application of Lightning Strike Protection on Thermoplastic Structures by Automated Fiber Placement Presentation held at the virtual IHTEC 2020 conference. Presents the advances in automated lay-up of lightning strike protection and the required materials.
description: Acknowledgement This project has received funding from the Clean Sky 2 Joint Undertaking (JU) under grant agreement No 945583. The JU receives support from the European Union's Horizon 2020 research and innovation programme and the Clean Sky 2 JU members other than the Union. Disclaimer The results, opinions, conclusions, etc. presented in this work are those of the author(s) only and do not necessarily represent the position of the JU; the JU is not responsible for any use made of the information contained herein.
Oncogenic Neu/ErbB-2 Increases Ets, AP-1, and NF-B-dependent Gene Expression, and Inhibiting Ets Activation Blocks Neu-mediated Cellular Transformation
Creators: name: Der, Channing J.
name: Oshima, Robert G.
name: Solski, Patricia A.
name: García-Ramírez, José J.
name: Galang, Christina K.
name: Hauser, Craig A.
name: Neznanov, Nickolay N.
name: Westwick, John K.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: Overexpression of Neu (ErbB-2/HER2) is found in approximately 20% of breast tumors. Activation of Neu by a point mutation (NeuT) causes constitutive tyrosine kinase activity of this transmembrane receptor and transforming activity in fibroblasts. To identify downstream targets of Neu, we have analyzed the ability of Neu to activate gene expression. Expression of NeuT, but not normal Neu, caused transcriptional activation of Ets, AP-1, or NF-kappaB-dependent reporter genes. Dominant inhibitory Ras or Raf mutants blocked the Neu-mediated transcriptional activation, confirming that Ras signaling pathways were required for this activation. Analysis with Ets2 mutants indicated that activation of Ets2 transcriptional activity mediated by NeuT or oncogenic Ras required phosphorylation of the same Ets2 residue, threonine 72. Cotransfection of dominant inhibitory Ets2 mutants specifically blocked NeuT-mediated activation of Ets-dependent reporter genes. Furthermore, in focus formation assays using NIH 3T3 cells, the transforming activity of NeuT was inhibited 5-fold when NeuT was cotransfected with a dominant negative Ets2 mutant. However, parallel colony formation assays showed that the Ets2 dominant negative mutant did not inhibit the growth of normal cells. Together, these data show that NeuT activates a variety of transcription factor families via the Ras signaling pathway and that Ets activation is required for NeuT-mediated cellular transformation. Thus, downstream targets of Neu, including Ets transcription factors, may be useful points for therapeutic intervention in Neu/ErbB-2-associated cancers.
(2011) Frances [Stead]
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Demokratie und Menschenrechte im Europa-Asien-Dialog
Creators: name: Loewen, Howard
Publisher: name: ASIEN: The German Journal on Contemporary Asia
Year: 2022

description: This paper addresses the question of whether differences between European and Asian forms and principles of state interaction can account for interregional cooperation problems in the areas of human rights and democracy. Whereas the EU favours formal, binding and thus supranational approach to co-operation, the Association of Southeast Asian Nations (ASEAN) is based on informal, non-binding, intergovernmental forms of co-operation. Based on empirical evidence taken from human rights and democracy dialogues between the EU and ASEAN on the one hand and the institutional context of the Asia-Europe Meeting (ASEM) on the other, the author argues that clash of co-operation cultures basically occurs in both institutional forms of interregional co-operation between Asia and Europe, with slight variations due to the institutional context. While clashes over democracy and human rights between the EU and ASEAN have led to complete standstill of co-operation, the flexible institutional mechanisms of ASEM seem to mitigate the negative effects of such dialogues at first sight. Yet, informality does not remove the issues from the agenda, as the recurrent disputes over Burma's participation in ASEM clearly indicate. These issues therefore have to be tackled in formal, institutionalised manner if ASEM wants to become what it aspires to be functioning link between Asia and Europe in the system of global governance.
description: ASIEN: The German Journal on Contemporary Asia, Nr. 95 (2005): ASIEN (April)
The American Soldier in World War II: Equipment and Supplies
Creators: name: Research Branch, Information
Publisher: name: Roper Center for Public Opinion Research
Year: 2020

description: Rank in military; branch of army; military occupation; length of time overseas; length of time with field jacket; types of jackets; raincoats; all-around winter coats; long woolen underwear; blankets and respondent's warmth; infantry field pack; musette bag; gas masks; field rations; halazone tablets; satisfaction of canteen; footwear; leggings; helmets.
gesellschaftliche Heterogenität der Länder Ostasiens
Creators: name: Ziltener, Patrick
Publisher: name: ASIEN: The German Journal on Contemporary Asia
Year: 2022

description: The critical examination of the ethnic, linguistic and religious heterogeneity of the countries of sample is now standard procedure in cross-national analysis. This article compares and re-evaluates the heterogeneity values of East Asian countries provided by new data sets as part of reference sample of 90 African and Asian countries. The different heterogeneity indices correlate highly, but case analyses show that there are markedly diverging codings in some countries cases. Thus, the ethnic heterogeneity of the Philippines and that of Taiwan have been estimated quite differently. Regarding religious heterogeneity, the syncretistic religious practices dominating in East Asia pose fundamental problem for coding and index construction. Two groups of countries can be differentiated: the more homogeneous Northeast Asian countries and the more heterogeneous Southeast Asian states, while Cambodia has level of societal heterogeneity corresponding more to that of the first group and Vietnam occupies position lying between the two groups.
description: ASIEN: The German Journal on Contemporary Asia, Nr. 95 (2005): ASIEN (April)
Synthesis of novel heterocyclic compounds bearing tetralin moiety of potential anticancer activity targeting the intrinsic apoptotic pathway
Creators: name: Hamdy, Nehal A.
name: Sayed, Mardia T. El
name: Hussein, Hoda A. R.
name: Mounier, Marwa M.
name: Anwar, Manal M.
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Biochemistry
subject: Cell Biology
subject: Molecular Biology
subject: Neuroscience
subject: Pharmacology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Developmental Biology
subject: Cancer
subject: 111714 Mental Health
subject: FOS: Health sciences
subject: Hematology
subject: 110309 Infectious Diseases

description: This study deals with the readily reaction of the enaminone derivative 3 with different reagents to give the corresponding pyrazolo[3,4-b]pyridine, pyrazolo[1,5-a]pyrimidine, pyrazole, pyrazolopyridazine, dienamide, and pyranone derivatives. In-vitro cytotoxic screening was performed for all analogs against several human cancer cell lines. Compound 21 showed significant anti-breast cancer activity and a safety profile against the normal human cell lines (BJ-1). Furthermore, compound 21 was chosen as a representative example to study its potential apoptotic mechanism in the MCF-7 cancer cell line. It enhanced DNA fragmentation alongside its characteristic effect on tubulin polymerization. In addition, compound 21 up-regulated the pro-apoptotic Bax protein, decreased the Bcl-2 anti-apoptotic protein, and increased p53 and Caspase-7 levels confirming its mitochondrial intrinsic apoptotic activation.
Vom Nichtimmigrationsland zum Immigrationsland
Creators: name: Chiavacci, David
Publisher: name: ASIEN: The German Journal on Contemporary Asia
Year: 2022

description: Japan has often been regarded as an exception among advanced industrialised economies. The lack of labour immigration from abroad in the post-war era up to the late 1970s seems to be an example of this singularity. National factors such as the large labour pool in Japan agricultural sector at the beginning of the high-growth era, mono-ethnic self-definition of Japan as nation and the influence of the strong developmental state have all been proposed by experts as explanations of the absence of immigration movements. This article argues that Japan apparent uniqueness regarding immigration needs to be analysed in regional context; by taking this approach, Japan can be seen to have followed the general development in East Asia. Parallel to East Asia change from non-migration to migration region, Japan turned from non-immigration country to land of immigration. Japan has become partial exception from regional viewpoint only because of the return migration of Japanese emigrants and their descendants from South America after the reform of Japanese migration regulations in 1990.
description: ASIEN: The German Journal on Contemporary Asia, Nr. 95 (2005): ASIEN (April)
Overexpression of Parathyroid Hormone-related Protein in the Pancreatic Islets of Transgenic Mice Causes Islet Hyperplasia, Hyperinsulinemia, and Hypoglycemia
Creators: name: Stewart, Andrew F.
name: Zawalich, Walter
name: D'Ercole, A. Joseph
name: Philbrick, William
name: Dann, Pamela
name: Madlener, Alex L.
name: Burtis, William J.
name: Zawalich, Kathleen
name: Cavaliere, Christi
name: Vasavada, Rupangi C.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: Parathyroid hormone-related protein (PTHrP) is produced by the pancreatic islet. It also has receptors on islet cells, suggesting that it may serve a paracrine or autocrine role within the islet. We have developed transgenic mice, which overexpress PTHrP in the islet through the use of the rat insulin II promoter (RIP). Glucose homeostasis in these mice is markedly abnormal; RIP-PTHrP mice are hypoglycemic in the postprandial and fasting states and display inappropriate hyperinsulinemia. At the end of a 24-hour fast, blood glucose values are 49 mg/dl in RIP-PTHrP mice, as compared to 77 mg/dl in normal littermates; insulin concentrations at this time are 6.3 and 3.9 ng/ml, respectively. Islet perifusion studies failed to demonstrate abnormalities in insulin secretion. In contrast, quantitative islet histomorphometry demonstrates that the total islet number and total islet mass are 2-fold higher in RIP-PTHrP mice than in their normal littermates. PTHrP very likely plays a normal physiologic role within the pancreatic islet. This role is most likely paracrine or autocrine. PTHrP appears to regulate insulin secretion either directly or indirectly, through developmental or growth effects on islet mass. PTHrP may have a role as an agent that enhances islet mass and/or enhances insulin secretion.
(2012) Benjamin Robinson
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Overproduction, Purification, and Characterization of the XPC Subunit of the Human DNA Repair Excision Nuclease
Creators: name: Reardon, Joyce T.
name: Mu, David
name: Sancar, Aziz
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: Xeroderma pigmentosum complementation group C gene (XPC) encodes a protein of 125 kDa which is present in a tight complex with a 58-kDa protein encoded by the human homolog of the yeast RAD23 gene, HHR23B (Masutani, C., Sugasawa, K., Yanagisawa, J., Sonoyama, T., Ui, M., Enomoto, T., Takio, K., Tanaka, K., van der Spek, P. J., Bootsma, D., Hoeijmakers, J. H. J., and Hanaoka, F.(1994) EMBO J. 13, 1831-1843). The XPC-HHR23B complex is required for excision of thymine dimers from DNA in a human excision nuclease system reconstituted from purified proteins. In order to understand the role of the XPC-HHR23B complex in excision repair, we have overexpressed each subunit alone and the heterodimer in heterologous systems, purified them, and characterized their biochemical properties. We find that both XPC and the heterodimer bind DNA with high affinity and UV-damaged DNA with slightly higher preference. Surprisingly, we find that the XPC subunit alone is sufficient for reconstitution of the human excision nuclease and that the HHR23B subunit has no detectable effect on the excision activity of the reconstituted system.
Purification and G Protein Subunit Regulation of a Phospholipase C-β from Xenopus laevis Oocytes
Creators: name: Paterson, Andrew
name: Filtz, Theresa M.
name: Kendall Harden, T.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: Xenopus oocytes exhibit both pertussis toxin-sensitive and -insensitive inositol lipid signaling responses to G protein-coupled receptor activation. The G protein subunits Galphai, Galphao, Galphaq, Galphas, and Gbetagamma all have been proposed to function as activators of phospholipase C in oocytes. Ma et al. (Ma, H.-W., Blitzer, R. D., Healy, E. C., Premont, R. T., Landau, E. M., and Iyengar, R. J. Biol. Chem. 268, 19915-19918) cloned a Xenopus phospholipase C (PLC-betaX) that exhibits homology to the PLC-beta class of isoenzymes. Although this enzyme was proposed to function as a signaling protein in the pertussis toxin-sensitive inositol lipid signaling pathway of oocytes, its regulation by G protein subunits has not been directly assessed. As such we have utilized baculovirus-promoted overexpression of PLC-betaX in Sf9 insect cells and have purified a recombinant 150-kDa isoenzyme. PLC-betaX catalyzes hydrolysis of phosphatidylinositol(4,5)bisphosphate and phosphatidylinositol(4)monophosphate, and reaction velocity is dependent on Ca2+. Recombinant PLC-betaX was activated by both Galphaq and Gbetagamma. PLC-betaX exhibited a higher apparent affinity for Galphaq than Gbetagamma, and Galphaq was more efficacious than Gbetagamma at lower concentrations of PLC-betaX. Relative to other PLC-beta isoenzymes, PLC-betaX was less sensitive to stimulation by Galphaq than PLC-beta1 but similar to PLC-beta2 and PLC-betaT. PLC-betaX was more sensitive to stimulation by Gbetagamma than PLC-beta1 but less sensitive than PLC-beta2 and PLC-betaT. In contrast PLC-betaX was not activated by the pertussis toxin substrate G proteins Galphai1, Galphai2, Galphai3, or Galphao. These results are consistent with the idea that PLC-betaX is regulated by alpha-subunits of the Gq family and by Gbetagamma and do not support the idea that alpha-subunits of pertussis toxin-sensitive G proteins are directly involved in regulation of this protein.
Lorentzian wormholes in Eddington-inspired Born-Infeld gravity
Creators: name: Shaikh, Rajibul
Publisher: name: arXiv
Year: 2015
Subjects: subject: General Relativity and Quantum Cosmology (gr-qc)
subject: High Energy Physics - Theory (hep-th)
subject: FOS: Physical sciences

description: We show that it is possible to construct a wide class of Lorentzian wormholes in Eddington-inspired Born-Infeld gravity with a stress energy which does not violate the weak or null energy condition. The wormholes exist in a certain region of the parameter space. In fact, it is shown that there is a critical value of a parameter defined in our work, below which we have wormholes. Above the critical value, we have a regular black hole spacetime. We put a restriction on the equation of state parameter $α$ ($p_θ=αρ$) to have wormholes. We also put a lower limit on both the theory parameter $|κ|$ and the throat radius, to restrict the tidal acceleration (at the throat) below one Earth gravity. As a special case of our general solution, we retrieve the wormhole supported by an electric field for a charge-to-mass ratio greater than the critical value $\left(\frac{Q}{M}\right)_c\approx 1.144$.
description: 15 pages, 5 figures, revised version accepted for publication in Phys. Rev. D
Ras Interaction with Two Distinct Binding Domains in Raf-1 5 Be Required for Ras Transformation
Creators: name: Campbell, Sharon L.
name: Der, Channing J.
name: Khosravi-Far, Roya
name: Drugan, Jonelle K.
name: White, Michael A.
name: Hwan, Yu Wen
name: Sung, Ying Ju
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: Although Raf-1 is a critical Ras effector target, how Ras mediates Raf-1 activation remains unresolved. Raf-1 residues 55-131 define a Ras-binding domain essential for Raf-1 activation. Therefore, our identification of a second Ras-binding site in the Raf-1 cysteine-rich domain (residues 139-184) was unexpected and suggested a more complex role for Ras in Raf-1 activation. Both Ras recognition domains preferentially associate with Ras-GTP. Therefore, mutations that impair Ras activity by perturbing regions that distinguish Ras-GDP from Ras-GTP (switch I and II) may disrupt interactions with either Raf-1-binding domain. We observed that mutations of Ras that impaired Ras transformation by perturbing its switch I (T35A and E37G) or switch II (G60A and Y64W) domain preferentially diminished binding to Raf-1-(55-131) or the Raf-1 cysteine-rich domain, respectively. Thus, these Ras-binding domains recognize distinct Ras-GTP determinants, and both may be essential for Ras transforming activity. Finally, since Ha-Ras T35A and E37G mutations prevent Ras interaction with full-length Raf-1, we suggest that Raf-Cys is a cryptic binding site that is unmasked upon Ras interaction with Raf-1-(55-131).
Time-evolution of the GaAs(0 0 1) pre-roughening process
Creators: name: Ding, Z.
name: Bullock, D. W.
name: Thibado, P. M.
name: LaBella, V. P.
name: Mullen, K.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Mesoscale and Nanoscale Physics (cond-mat.mes-hall)
subject: Materials Science (cond-mat.mtrl-sci)
subject: FOS: Physical sciences

description: The GaAs(0 0 1) surface is observed to evolve from being perfectly flat to a surface half covered with one-monolayer high spontaneously formed GaAs islands. The dynamics of this process are monitored with atomic-scale resolution using scanning tunneling microscopy. Surprisingly, pit formation dominates the early stages of island formation. Insight into the nucleation process is reported.
description: 8 pages, 4 figures
A complete Randomized Benchmarking Protocol accounting for Leakage Errors
Creators: name: Chasseur, T.
name: Wilhelm, F. K.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Quantum Physics (quant-ph)
subject: FOS: Physical sciences

description: Randomized Benchmarking allows to efficiently and scalably characterize the average error of an unitary 2-design such as the Clifford group $\mathcal{C}$ on a physical candidate for quantum computation, as long as there are no non-computational leakage levels in the system. We investigate the effect of leakage errors on Randomized Benchmarking induced from an additional level per physical qubit and provide a modified protocol that allows to derive reliable estimates for the error per gate in their presence. We assess the variance of the sequence fidelity corresponding to the number of random sequences needed for valid fidelity estimation. Our protocol allows for gate dependent error channels without being restricted to perturbations. We show that our protocol is compatible with Interleaved Randomized Benchmarking and expand to benchmarking of arbitrary gates. This setting is relevant for superconducting transmon qubits, among other systems.
Supporting figure S1 from Analysis of modularity and integration suggests evolution of dragonfly wing venation mainly in response to functional demands.
Creators: name: Blanke, Alexander
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: Evolutionary Biology
subject: FOS: Biological sciences
subject: 110601 Biomechanics
subject: FOS: Health sciences

description: Phylomorphospace plots of the first three principal components of the uncorrected (A+C) and allometry and phylogeny corrected (B+D) landmark datasets for the forewings (A+B) and hindwings (C+D). The branching pattern is based on the phylogeny of Letsch et al. (2016).
Reaction Mechanism of Human DNA Repair Excision Nuclease
Creators: name: Hsu, David S.
name: Sancar, Aziz
name: Mu, David
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: Nucleotide excision repair consists of removal of the damaged nucleotide(s) from DNA by dual incision of the damaged strand on both sides of the lesion, followed by filling of the resulting gap and ligation. In humans, 14-16 polypeptides are required for the dual incision step. We have purified the required proteins to homogeneity and reconstituted the dual incision activity (excision nuclease) in a defined enzyme/substrate system. The system was highly efficient, removing >30% of the thymine dimers under optimal conditions. All of the six fractions that constitute the excision nuclease were required for dual incision of the thymine dimer substrate. However, when a cholesterol-substituted oligonucleotide was used as substrate, excision occurred in the absence of the XPC-HHR23B complex, reminiscent of transcription-coupled repair in the XP-C mutant cell line. Replication protein A is absolutely required for both incisions. The XPG subunit is essential to the formation of the preincision complex, but the repair complex can assemble and produce normal levels of 3'-incision in the absence of XPF-ERCC1. Kinetic experiments revealed that the 3'-incision precedes the 5'-incision. Consistent with the kinetic data, uncoupled 5'-incision was never observed in the reconstituted system. Two forms of TFIIH were used in the reconstitution reaction, one containing the CDK7-cyclin H pair and one lacking it. Both forms were equally active in excision. The excised oligomer dissociated from the gapped DNA in a nucleoprotein complex. In total, these results provide a detailed account of the reactions occurring during damage removal by human excision nuclease.
Greener and rapid synthesis of benzal-based Schiff base ligands as an efficient antibacterial, antioxidant, and anticancer agent
Creators: name: Saranya, G.
name: Devendraprasad, K.
name: Jayanthi, P.
name: Shanmugapriya, P.
name: Bhuvaneshwari, N.
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Biophysics
subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Pharmacology
subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Cancer

description: Using an ecologically friendly sonication protocol, certain benzal-based Schiff base ligands were produced by combining benzaldehyde and its para-derivatives with 2-(2-methoxyphenoxy)ethanamine. The reaction lasts around 2 min and yields 86.27% of the Schiff base ligands. Human tumor cell lines were used to test the ligand anticancer action and the results showed that MPEA-ANI (Antiproliferation of Breast cancer MDA MB 231 is 71% and Lung cancer A 549 is 85%) exhibits significant inhibition against both cell lines. Through the scavenging effects of ligands on DPPH, hydrogen peroxide, and nitric oxide radicals, the ligands antioxidant properties were investigated. The bacteria strains used to assess the antibacterial effectiveness of ligands were Bacillus subtilis, Staphylococcus aureus (Gram-positive bacteria), and Escherichia coli (Gram-negative bacterium). The ligands were described using elemental analysis, FT-IR, 1H NMR, 13C NMR, and mass spectroscopy.
A monad measure space for logarithmic density
Creators: name: Di Nasso, Mauro
name: Goldbring, Isaac
name: Jin, Renling
name: Leth, Steven
name: Lupini, Martino
name: Mahlburg, Karl
Publisher: name: arXiv
Year: 2015
Subjects: subject: Logic (math.LO)
subject: Combinatorics (math.CO)
subject: Dynamical Systems (math.DS)
subject: Number Theory (math.NT)
subject: FOS: Mathematics

description: We provide a framework for proofs of structural theorems about sets with positive Banach logarithmic density. For example, we prove that if $A\subseteq \mathbb{N}$ has positive Banach logarithmic density, then $A$ contains an approximate geometric progression of any length. We also prove that if $A,B\subseteq \mathbb{N}$ have positive Banach logarithmic density, then there are arbitrarily long intervals whose gaps on $A\cdot B$ are multiplicatively bounded, a multiplicative version Jin's sumset theorem. The main technical tool is the use of a quotient of a Loeb measure space with respect to a multiplicative cut.
description: 26 pages
Supporting figure S2 from Analysis of modularity and integration suggests evolution of dragonfly wing venation mainly in response to functional demands.
Creators: name: Blanke, Alexander
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: Evolutionary Biology
subject: FOS: Biological sciences
subject: 110601 Biomechanics
subject: FOS: Health sciences

description: Summary of the outlier analysis of wing shapes for fore- and hindwings before and after allometric and phylogenetic correction. The identified outlier species correspond to the triangles in Figure 2.
Replication Protein A Confers Structure-specific Endonuclease Activities to the XPF-ERCC1 and XPG Subunits of Human DNA Repair Excision Nuclease
Creators: name: Bessho, Tadayoshi
name: Mu, David
name: Matsunaga, Tsukasa
name: Sancar, Aziz
name: Park, Chi Hyun
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: XPF-ERCC1 and XPG proteins are nucleases that are involved in human nucleotide excision repair. In this study, we characterized the structure-specific junction-cutting activities of both nucleases using DNA substrates containing a bubble or loop structure. We found that the junction-cutting activities of XPF-ERCC1 and XPG were greatly stimulated by human replication protein A (RPA), while heterologous single-stranded DNA-binding proteins could not substitute for human RPA. To test for specific interaction between RPA and XPF-ERCC1 as is known to occur between RPA and XPG, we employed a pull-down assay with immobilized "bubble" substrate. We found that the binding of XPF-ERCC1 complex to the bubble substrate was enhanced by RPA, suggesting a possible mechanism for RPA in the excision nuclease system, that is the targeting of the nuclease subunits to their specific sites of action. Furthermore, the RPA-promoted junction cutting by XPF-ERCC1 and XPG nucleases was observed with "loop" substrates as well, raising the possibility that XPF-ERCC1, XPG, and RPA may function in removing loop structures from DNA, independent of the other subunits of the human excinuclease.
(2006) Hannah Nicholson
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Synthesis, characterization and biological evaluation of the novel chromenopyridothiazolopyrimidines and chromenopyridopyrimidothiazolo-pyrimidines
Creators: name: Alshaye, Najla A.
name: Ibrahim, Magdy A.
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Biochemistry
subject: 29999 Physical Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: Microbiology
subject: FOS: Biological sciences
subject: Pharmacology
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: Inorganic Chemistry
subject: Hematology

description: Friedländer reaction of 4-aminocoumarin-3-carboxaldehyde (1) with thiobarbituric acid provided 2-thioxochromenopyridopyrimidine 2 which employed as a synthetic intermediate to create a novel annulated chromenopyridothiazolopyrimidines. Treatment of compound 2 with a diversity of nucleophiles including; dibromoethane, oxalyl chloride, phenacyl bromide, chloroacetic acid, 3-chloropentanedione, ethyl 2-chloro-3-oxobutanoate, chloroacetonitrile and bromomalononitrile afforded chromenopyridothiazolopyrimidines 3–10, respectively. Acidic hydrolysis of carbonitrile 10 afforded the corresponding carboxamide derivative 11. Compound 11 reacted with some mono-electrophilic reagents namely acetyl chloride, benzoyl chloride, chloroacetyl chloride, triethyl orthoformate, diethyl carbonate, and carbon disulfide producing the first known chromeno[3′,4′:5,6]pyrido[2,3-d]pyrimido [4',5':4,5][1,3]thiazolo[3,2-a]pyrimidines 12–17. The antimicrobial and anticancer activities were estimated for the synthesized products and some of them appeared notable activities. The structural elucidation of the newly synthesized products was determined using analytical and spectroscopic data.
Site-directed Mutagenesis of Rabbit Proacrosin: IDENTIFICATION OF RESIDUES INVOLVED IN ZONA PELLUCIDA BINDING
Creators: name: Richardson, Richard T.
name: O'Rand, Michael G.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: The mammalian acrosomal sperm protease proacrosin plays a role in fertilization by proteolysis of the oocyte's outer investments. In addition to its serine protease activity, acrosin from several species is known to have binding activity for the zona pellucida, and this action may serve to anchor sperm during zona penetration. In this study, proacrosin was purified from acid extracts of rabbit sperm and shown to bind to homologous zona pellucida using an in vitro assay. Measurement of this binding activity indicated a high affinity saturable interaction with a KD = 1.4 x 10(-8) M. Using cDNAs obtained from previously cloned and sequenced rabbit proacrosin and a splice variant that encodes a shorter form of acrosin (Richardson, R. T., and O'Rand, M. G. (1994) Biochim. Biophys. Acta 1219, 215-218), constructs of various sizes were produced using polymerase chain reaction and expressed as recombinant proteins. In the same in vitro zona binding assay, a construct representing residues 1-279 of rabbit proacrosin was found to bind to zona with a high affinity similar to that of native proacrosin, KD = 2.1 x 10(-8) M. By making smaller recombinant fragments and assaying them for zona binding activity, the location of the binding site was mapped to residues 47-94. Protein modeling of rabbit proacrosin using chymotrypsinogen A as a three-dimensional model indicated that an exposed loop Asp35 to His40 in chymotrypsinogen A is extended with an additional five amino acid residues in rabbit proacrosin from Ile43 to His53 containing arginine residues Arg47, Arg50 and Arg51. Site-directed mutagenesis of arginine residues Arg50 and Arg51 to alanine produced a recombinant without significant zona binding activity. These results are consistent with the hypothesis that rabbit proacrosin contains a specific zona pellucida binding site and that the loop containing arginine residues 50 and 51 is critical for zona binding activity.
(2007) Joash Nicholson
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
The American Soldier in World War II: Trend Study on Attitudes
Creators: name: Research Branch, Information
Publisher: name: Roper Center for Public Opinion Research
Year: 2020

description: Rank in military; state in which respondent spend last five years of civilian life; length of time in army; length of time at station; branch of army; military occupation; pride in company; overseas combat v.s. overseas non-combat duty v.s. US duty; officer/enlisted men relations; soldier v.s. working a war job; good food in army; back to college after war; GI bill; show seen while in military; Red Cross job performance; Red Cross girls; refreshments served in canteens; Class F allotment; Class E allotment; Class B allotment; send pay home each month; best way to save money; war bonds; life insurance; 'soldiers deposit' account; CBI Round Up; pin-up pictures; Yank magazine.
Site-directed Mutagenesis of the Cysteine Residues in the Neurospora crassa Plasma Membrane H-ATPase
Creators: name: Mahanty, Sanjoy K.
name: Scarborough, Gene A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: A high-yield yeast expression system for site-directed mutagenesis of the Neurospora crassa plasma membrane H(+)-ATPase has recently been reported (Mahanty, S. K., Rao, U. S., Nicholas, R. A., and Scarborough, G. A. (1994) J. Biol. Chem. 269, 17705-17712). Using this system, each of the eight cysteine residues in the ATPase was changed to a serine or an alanine residue, producing strains C148S and C148A, C376S and C376A, C409S and C409A, C472S and C472A, C532S and C532A, C545S and C545A, C840S and C840A, and C869S and C869A, respectively. With the exception of C376S and C532S, all of the mutant ATPases are able to support the growth of yeast cells to different extents, indicating that they are functional. The C376S and C532S enzymes appear to be non-functional. After solubilization of the functional mutant ATPase molecules from isolated membranes with lysolecithin, all behaved similar to the native enzyme when subjected to glycerol density gradient centrifugation, indicating that they fold in a natural manner. The kinetic properties of these mutant enzymes were also similar to the native ATPase with the exception of C409A, which has a substantially higher Km. These results clearly indicate that none of the eight cysteine residues in the H(+)-ATPase molecule are essential for ATPase activity, but that Cys376, Cys409, and Cys532 may be in or near important sites. They also demonstrate that the previously described disulfide bridge between Cys148 and Cys840 or Cys869 plays no obvious role in the structure or function of this membrane transport enzyme.
Melting temperature of graphene
Creators: name: Los, J. H.
name: Zakharchenko, K. V.
name: Katsnelson, M. I.
name: Fasolino, Annalisa
Publisher: name: arXiv
Year: 2015
Subjects: subject: Mesoscale and Nanoscale Physics (cond-mat.mes-hall)
subject: FOS: Physical sciences

description: We present an approach to the melting of graphene based on nucleation theory for a first order phase transition from the 2D solid to the 3D liquid via an intermediate quasi-2D liquid. The applicability of nucleation theory, supported by the results of systematic atomistic Monte Carlo simulations, provides an intrinsic definition of the melting temperature of graphene, $ T_m $, and allows us to determine it. We find $T_m \simeq 4510$ K, about 250 K higher than that of graphite using the same interatomic interaction model. The found melting temperature is shown to be in good agreement with the asymptotic results of melting simulations for finite disks and ribbons of graphene. Our results strongly suggest that graphene is the most refractory of all known materials.
220907 Buffering AB-MODEL EQUATIONS.docx
Creators: name: WOLF, MATTHEW
Publisher: name: figshare
Year: 2022
Subjects: subject: 111603 Systems Physiology
subject: FOS: Basic medicine
subject: Physiology
subject: FOS: Biological sciences
subject: 60199 Biochemistry and Cell Biology not elsewhere classified

description: Physicochemical blood model equations
(2008) Sarah [Nicholson]
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Specific Interaction of Glyceraldehyde 3-Phosphate Dehydrogenase with the 5′-Nontranslated RNA of Hepatitis A Virus
Creators: name: Schultz, Derk E.
name: Hardin, Charles C.
name: Lemon, Stanley M.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: Initiation of translation of hepatitis A virus (HAV) RNA occurs by internal entry and is likely to involve the interaction of trans-acting cellular protein factors with cis-acting structural elements of an internal ribosomal entry segment (IRES) within the 5'-nontranslated RNA. To characterize interactions between African green monkey kidney (BS-C-1) cell proteins and the predicted stem-loop IIIa (nucleotides 155-235) located at the 5' border of the HAV IRES, we utilized an electrophoresis mobility shift assay (EMSA) to identify a 39-kDa RNA-binding protein (p39). Amino-terminal amino acid sequencing of highly purified p39 revealed absolute identity with human glyceraldehyde 3-phosphate dehydrogenase (GAPDH). The identity of p39 as simian GAPDH was further confirmed by antigenic and biochemical similarities between p39 and human GAPDH. Analysis of the RNA binding properties of simian GAPDH revealed that this cellular protein interacts with two additional sites in the HAV 5'-nontranslated RNA, one located between nucleotides 1-148 and the other between nucleotides 597-746. Competitive EMSAs also demonstrated that GAPDH and human polypyrimidine tract-binding protein, a putative picornavirus translation initiation factor, compete with each other for binding to stem-loop IIIa, suggesting that the relative cytoplasmic abundance of GAPDH and polypyrimidine tract-binding protein in individual cell-types may be an important determinant of viral translation activity. Human GAPDH was found to destabilize the folded structure of the stem-loop IIIa RNA based upon observed decreases in the circular dichroism spectra of this RNA following binding of the protein. This RNA helix-destabilizing activity of GAPDH could directly influence IRES-dependent translation and/or replication of picornavirus RNA.
Structural Integrity of the -Carboxyglutamic Acid Domain of Human Blood Coagulation Factor IXa Is Required for Its Binding to Cofactor VIIIa
Creators: name: Larson, Peter J.
name: Stanfield-Oakley, Sherry A.
name: VanDusen, William J.
name: Smith, Kenneth J.
name: Kasper, Carol K.
name: High, Katherine A.
name: Monroe, Dougald M.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: This report describes the analysis of a novel mutant human factor IX protein from a patient with hemophilia B (factor IX activity <1%; factor IX antigen 45%). Enzymatic amplification of all eight exons of the factor IX gene followed by direct sequence analysis reveals a single nucleotide change (a guanine --> adenine transition) in exon 2 at nucleotide 6409 which results in a glycine --> arginine substitution at amino acid 12 in the gamma-carboxyglutamic acid rich (Gla) domain of the mature protein. Factor IX was isolated by immunoaffinity chromatography from plasma obtained from the proband. The purified protein is indistinguishable from normal factor IX by polyacrylamide gel electrophoresis. Characterization of the variant in purified component assays reveals that it is activated normally by its physiologic activator factor XIa, but its phospholipid-dependent activation by the factor VIIa-tissue factor complex is diminished. In the presence of phospholipid and 5 mM Ca2+, the activities of variant and normal plasma-derived factor IX are similar; however, in the presence of activated factor VIIIa (intrinsic tenase complex), the normal augmentation of the cleavage of the specific substrate of factor IX, factor X, is not observed. The determination of the association constants for normal and variant factor IXa with factor VIIIa shows that the affinity of the activated variant factor IX for the cofactor factor VIIIa is 172-fold lower than normal. Competition studies using active site-inactivated factor IXas in the intrinsic tenase complex confirm that the defect in the variant protein is in its binding to factor VIIIa. We conclude that the structural integrity of the Gla domain of human factor IX is critical for the normal binding of factor IXa to factor VIIIa in the intrinsic tenase complex. In addition, a glycine at amino acid 12 is necessary for normal activation of factor IX by the factor VIIa-tissue factor complex.