Trichoderma subalni Y. B. Zhang & W. Y. Zhuang HMAS 2018, sp. nov.
Creators: name: Zhang, Yu-Bo
name: Zhuang, Wen-Ying
Publisher: name: Zenodo
Year: 2018
Subjects: Biodiversity
Taxonomy
Fungi
Ascomycota
Pezizomycetes
Pezizales
Pezizaceae
Trichoderma
Trichoderma subalni

description: Trichoderma subalni Y.B. Zhang & W.Y. Zhuang, sp. nov. (Fig. 4)   
Fungal names: FN 570574  
 Etymology:— The specific epithet refers to the morphological similarity to T. alni.   
 Type:— CHINA. Tibet: Bomê, Gangxiang Nature Reserve, alt. 2700 m, on rotten wood, 19 Sep 2016, Y.B. Zhang, Z.Q. Zeng, Z.H. Yu, H.D. Zheng, K. Chen & X.C. Wang 11017 (holotype HMAS 275683, ex-type culture HMAS 247267).   
 Stromata solitary or aggregated in small groups, pulvinate, outline irregular, ca. 1–3 mm in diam., 1 mm high. Surface yellowish brown to reddish brown, becoming dark in 3% KOH. Ostioles inconspicuous. In section, cortical tissue of textura angularis, 12–25 μm thick, cells luteofulvous, thick-walled, (2–)2.9–8(–11) × 1.5–6 μm (n = 30); subcortical tissue of textura angularis, cells slightly orange, thin-walled, 6–10 × 4–6 μm; subperithecial tissue of textura epidermoidea, cells hyaline, thin-walled, 9–33 × 4.5–16 μm; tissue at the base of textura angularis mixed with hyphae, cells hyaline to light orange, thin-walled, 6–18 × 4–10 μm, hyphae hyaline to light orange, thin-walled, 2.8–9 μm wide. Perithecia subglobose or flask-shaped, 191–243 × 88–176 μm; peridium light orange to orange-yellow, 3.5–12 μm thick at flanks, 5–11 μm thick at the base. Ostioles 50–77 μm high, 19–41 μm wide at the apex. Asci subcylindrical, 76–100 × 4–5 μm, with a stipe 9–17 μm long. Ascospores green, verruculose, dimorphic, distal cells globose or oblong, 3–5 × 3–4.5 μm, L/W 1–1.4(–1.5); proximal cells subglobose to ellipsoid, 3–5 × 2.5–3.5 μm, L/ W 1.1 –1.7(–1.9).   
Colony on CMD 41–48 mm in radius after 72 h at 25 °C, covering the plate after 5 days. Colony circular, semitranslucent, aerial hyphae loosely distributed. Conidiation noted after 4 days on aerial hyphae and in pustules. No diffusing pigment produced; no distinct odor detected.  
Colony on PDA 27–43 mm in radius after 72 h at 25 °C, covering the plate after 7 days. Colony outline irregular, with margin lobed, zonate, aerial hyphae abundant, downy. Conidiation noted after 2 days on aerial hyphae near the plug. Yellowish brown pigment produced; no distinct odor detected.  
 Colony on SNA 26–47 mm in radius after 72 h at 25 °C, covering the plate after 7 days. Colony outline irregular, hyaline, margin lobed, inconspicuously zonate, aerial hyphae sparsely disposed. Conidiation noted after 4 days on aerial hyphae. Conidiophores trichoderma-like, often re-branching once. Phialides solitary or commonly divergent in whorls of 2–4, subulate, lageniform or ampulliform, 7–15 × 2–3.5 μm, L/W 2–5.3, 1.5–3 μm wide at the base. Conidia green, surface smooth, globose, oblong or ellipsoid, 2.5–5 × 2–3 μm, L/W 1–1.6. No diffusing pigment produced; no distinct odor detected.   
 Additional specimen examined:— CHINA. Tibet: Bomê, Gangxiang Nature Reserve, alt. 2700 m, on rotten wood, 19 Sep 2016, Y.B. Zhang, Z.Q. Zeng, Z.H. Yu, H.D. Zheng, K. Chen & X.C. Wang 11020 (HMAS 275684, culture HMAS 247268).   
 Notes:— Trichoderma subalni is morphologically similar to T. alni in stroma appearance, anatomical structure and size of ascospores. But T. alni differs in somewhat loose conidiophore branching pattern, slightly larger ascospores [proximal cells (3.5–)4.5–6(–7.2) × (2.7–)3–3.5(–4) μm], and sequence data (Jaklitsch et al. 2008). Phylogenetically, T. subalni is closely related to T. zayuense, although the latter grows faster, produces chlamydospores on PDA and SNA, and possesses wider phialides (3.1–4.2 × 2.8–3.8 μm) (Chen & Zhuang 2017a). There are 30–40 bp divergences in TEF 1 sequence between T. subalni and T. zayuense.
description: Published as part of Zhang, Yu-Bo & Zhuang, Wen-Ying, 2018, New species of Trichoderma in the Harzianum, Longibrachiatum and Viride clades, pp. 131-142 in Phytotaxa 379 (2) on page 139, DOI: 10.11646/phytotaxa.379.2.1, http://zenodo.org/record/13724719