title: Ð¡Ð¾Ð²Ð¼ÐµÑÑÐ½Ð¾Ðµ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÑÐ¸ÑÐ¾Ð¿Ð°ÑÐ¾Ð³ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð³ÑÐ¸Ð±Ð° Beauveria bassiana ÑÑ. 2301 Ð¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð¸ Bacillus thuringiensis ÑÑ. 1903 Ð´Ð»Ñ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ½Ð¾Ð³Ð¾ Ð±Ð¸Ð¾Ð¸Ð½ÑÐµÐºÑÐ¸ÑÐ¸Ð´Ð° ÐºÐ¸ÑÐµÑÐ½Ð¾-ÐºÐ¾Ð½ÑÐ°ÐºÑÐ½Ð¾Ð³Ð¾ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Joint cultivation of the phytopathogenic fungus Beauveria bassiana pcs. 2301 and the bacterium Bacillus thuringiensis pcs. 1903 to obtain a complex bioinsecticide of intestinal contact action
Creators: name: ÐÐ²Ð°Ð½Ð¾Ð², ÐÑÑÐµÑÐ»Ð°Ð²
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð±Ð¸Ð¾Ð¸Ð½ÑÐµÐºÑÐ¸ÑÐ¸Ð´
Ð¿Ð¾Ð»ÑÑÐ¸Ð½ÑÐµÑÐ¸ÑÐµÑÐºÐ¸Ðµ Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½ÑÐµ ÑÑÐµÐ´Ñ
ÑÐ¾Ð²Ð¼ÐµÑÑÐ½Ð¾Ðµ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
Ð»Ð¸Ð¼Ð¾Ð½Ð½Ð°Ñ ÐºÐ¸ÑÐ»Ð¾ÑÐ°
Ð½ÐµÐ¸Ð¾Ð½Ð¾Ð³ÐµÐ½Ð½Ð¾Ðµ Ð¿Ð¾Ð²ÐµÑÑ Ð½Ð¾ÑÑÐ½Ð¾-Ð°ÐºÑÐ¸Ð²Ð½Ð¾Ðµ Ð²ÐµÑÐµÑÑÐ²Ð¾
Ð±Ð¸Ð¾ÑÐµÐ°ÐºÑÐ¾Ñ
bioinsecticide
semi-synthetic nutrient media
co-cultivation
citric acid
nonionic surfactant
bioreactor

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° ÑÐ¾Ð²Ð¼ÐµÑÑÐ½Ð¾Ð¼Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÑÐ¸ÑÐ¾Ð°ÑÐ¾Ð³ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð³ÑÐ¸Ð±ÐºÐ° B.bassiana ÑÑÐ°Ð¼Ð¼ EK2302 Ð¸ Bacillus thuringiensis ÑÑ. 1903, Ñ ÑÐµÐ»ÑÑ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ½Ð¾Ð³Ð¾ Ð±Ð¸Ð¾Ð¸Ð½ÑÐµÐºÑÐ¸ÑÐ¸Ð´Ð° ÐºÐ¸ÑÐµÑÐ½Ð¾-ÐºÐ¾Ð½ÑÐ°ÐºÑÐ½Ð¾Ð³Ð¾ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ. ÐÐ±ÑÐµÐºÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ - ÑÑÐ°Ð¼Ð¼Ñ Beauveria bassiana ÑÑ. EK2302 Ð¸ Bacillus thuringiensis ÑÑ ÐÐ1903 Ð¸Ð· ÐºÐ¾Ð»Ð»ÐµÐºÑÐ¸Ð¸ Ð¿Ð¾Ð»ÐµÐ·Ð½ÑÑ Ð¼Ð¸ÐºÑÐ¾Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð¾Ð² Ð¤ÐÐÐÐ£ ÐÐÐÐÐ¡Ð¥Ð. Ð Ð¿ÑÐ¾ÑÐµÑÑÐµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¾ Ð¸Ð·ÑÑÐµÐ½Ð¾, ÐºÐ°Ðº ÑÐ¾Ð²Ð¼ÐµÑÑÐ½Ð¾Ðµ Ð²ÑÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ðµ 0,4 % Ð¸ 3,47 % Ð³ÑÐ¸Ð±ÐºÐ° B.bassiana Ð¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð¸ B.thuringiensis Ð¾Ñ Ð¾Ð±ÑÐµÐ³Ð¾ Ð¾Ð±ÑÐµÐ¼Ð° Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ´Ñ Ð±Ð»Ð°Ð³Ð¾Ð¿ÑÐ¸ÑÑÐ½Ð¾ Ð²Ð»Ð¸ÑÑÑ Ð´ÑÑÐ³ Ð½Ð° Ð´ÑÑÐ³Ð°. ÐÑÐ»Ð¾ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¾, ÑÑÐ¾ Ð´Ð¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸Ðµ Ð»Ð¸Ð¼Ð¾Ð½Ð½Ð¾Ð¹ ÐºÐ¸ÑÐ»Ð¾ÑÑ Ð² ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ 0,25 % Ð¸ 0,5 % Ð¾Ñ Ð¾Ð±ÑÐµÐ³Ð¾ Ð¾Ð±ÑÐµÐ¼Ð° Ð²ÐµÑÐµÑÑÐ²Ð° ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ Ð¿ÑÐµÐ´Ð¾ÑÐ²ÑÐ°ÑÐ°ÐµÑ Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð±Ð¸Ð¾Ð¿Ð»ÑÐ½Ð¾Ðº. ÐÑÐ»Ð¾ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¾, ÑÑÐ¾ Ð²Ð½ÐµÑÐµÐ½Ð¸Ðµ 0,05 % Ð¾Ñ Ð¾Ð±ÑÐµÐ³Ð¾ Ð¾Ð±ÑÐµÐ¼Ð° Ð²ÐµÑÐµÑÑÐ²Ð° Ð½ÐµÐ¸Ð¾Ð½Ð¾Ð³ÐµÐ½Ð½Ð¾Ð³Ð¾ ÐÐÐ Ð² ÑÐ¾ÑÑÐ°Ð²Ðµ Ð¿ÑÐµÐ¿Ð°ÑÐ°Ñ Â«ÐÐ´Ð³ÐµÐ·Ð¸Ð²-ÐÐ¸Ð¾Â» Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÐµÑ ÐºÐ¾Ð½ÑÑÐ¾Ð»Ð¸ÑÐ¾Ð²Ð°ÑÑ ÑÐ¾ÑÑ Ð¸ Ð¾Ð±ÐµÑÐ¿ÐµÑÐ¸Ð²Ð°ÐµÑ ÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾ÑÑÑ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ° Ð¿ÑÐ¸ ÑÑÐ°Ð½ÐµÐ½Ð¸Ð¸. ÐÑÑÐ²Ð»ÐµÐ½Ñ Ð¾Ð¿ÑÐ¸Ð¼Ð°Ð»ÑÐ½ÑÐµ ÑÐ¾Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ñ Ð½ÐµÐ¸Ð¾Ð½Ð¾Ð³ÐµÐ½Ð½Ð¾Ð³Ð¾ ÐÐÐ, Ð² ÑÐ¾ÑÑÐ°Ð²Ðµ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ° Â«ÐÐ´Ð³ÐµÐ·Ð¸Ð²-ÐÐ¸Ð¾Â», Ð¸ Ð»Ð¸Ð¼Ð¾Ð½Ð½Ð¾Ð¹ ÐºÐ¸ÑÐ»Ð¾ÑÑ ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐ¾ÑÑÐ°Ð²Ð»ÑÑÑ 0,1 % Ð¸ 0,25 % Ð¾Ñ Ð¾Ð±ÑÐµÐ³Ð¾ Ð¾Ð±ÑÐµÐ¼Ð° Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ°. ÐÑÐ¸ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð¸Ð¸ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±Ð·Ð¾ÑÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ eLIBRARY.RU, Elsevier ScienceDirect, Scopus, PubMed, PubChem, Taylor &amp; Fransic Online, MDPI â Publisher of Open Access Journals. Ð¡ÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÑÑ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»Ð¸ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿Ð°ÐºÐµÑÐ° Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼ Microsoft Excel, GraphPad Prism Ð²ÐµÑÑÐ¸Ð¸ 8.0, ÑÐ·ÑÐºÐ° Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Python 3.10.
description: This work is devoted to the joint cultivation of the phytopathogenic fungus B.bassiana strain EK2302 and Bacillus thuringiensis, 1903, to obtain a complex bioinsecticide of intestinal contact action. The object of the study is the strains of Beauveria bassiana pcs. EK2302 and Bacillus thuringiensis pcs. EK1903 from the collection of beneficial micro-organisms of the Federal State Budgetary Scientific Institution VNIISHM. In the course of the study, it was studied how the joint cultivation of 0,4 % and 3.47% of the fungus B.bassiana and the bacterium B.thuringiensis from the total volume of the nutrient medium has a beneficial effect on each other. It was found that the addition of citric acid in concentrations of 0,25 % and 0,5 % of the total volume of the substance effectively prevents the for-mation of biofilms. It was determined that the introduction of 0,05 % of the total volume of non-ionic surfactant in the composition of Adhesive-Bio allows you to control growth and ensures the stability of the drug during storage. The optimal ratios of non-ionic surfactants in the composition of Adhe-sive-Bio and citric acid were revealed, which make up 0,1 % and 0,25 % of the total volume of the drug. These ratios allow you to most effectively prevent the formation of biofilms and control the growth of microorganisms during the storage of the biological product. The following databases were used to perform the literature review: eLibrary.RU, Elsevier ScienceDirect, Scopus, PubMed, PubChem, Taylor &amp; Fransic Online, MDPI â Publisher of Open Access Journals. Statistical processing of experimental data was carried out using the Microsoft Excel software package, GraphPad Prism version 8.0, and the Python 3.10 programming language.