title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² ÑÐµÐ¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÐºÐ»ÐµÑÐ¾Ðº Ð½Ð° Ð½Ð°ÑÑÑÐµÐ½Ð¸Ñ ÐºÐ°Ð»ÑÑÐ¸ÐµÐ²Ð¾Ð¹ ÑÐ¸Ð³Ð½Ð°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð² Ð¿Ð°ÑÐ¸ÐµÐ½Ñ-ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐ½ÑÑ Ð¼Ð¾Ð´ÐµÐ»ÑÑ Ð½ÐµÐ¹ÑÐ¾Ð´ÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸Ð²Ð½ÑÑ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ð¹
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: The influence of cell reprogramming methods on disturbances in calcium signaling in patient-specific models of neurodegenerative diseases
Creators: name: ÐÑÑÑÐ¸Ð½Ð¸Ð½Ð°, ÐÐ½Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: ÑÐµÐ¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐµ Ð¿Ð»ÑÑÐ¸Ð¿Ð¾ÑÐµÐ½ÑÐ½ÑÐµ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÐµ
Ð´ÐµÐ¿Ð¾-ÑÐ¿ÑÐ°Ð²Ð»ÑÐµÐ¼ÑÐ¹ Ð²Ñ Ð¾Ð´ ÐºÐ°Ð»ÑÑÐ¸Ñ
ÐºÐ°Ð»ÑÑÐ¸ÐµÐ²Ð°Ñ ÑÐ¸Ð³Ð½Ð°Ð»Ð¸Ð·Ð°ÑÐ¸Ñ
reprogramming
induced pluripotent stem cells
store operated calcium entry
calcium signaling

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description: The aim of this paper is to investigate the influence of integrative and non-integrative cell reprogramming methods on disturbances in calcium signaling in patient-specific models of neurodegenerative diseases. The following tasks were accomplished in the course of the study: 1. Compare depo-activated calcium entry in cell lines obtained using integrative and non-integrative reprogramming methods for juvenile Huntingtons disease model and healthy donor. 2. Compare potential-activated calcium entry in cell lines obtained using integrative and non-integrative reprogramming methods for juvenile Huntingtons disease model and healthy donor. 3. Compare the levels of proteins, activators of depo-activated channels, STIM1 and STIM2, and channel-forming protein Orai1 in cell lines obtained using integrative and non-integrative reprogramming methods for juvenile Huntingtons disease model and healthy donor. Based on the results, it can be concluded that depo- and potential-activated calcium entry, as well as the level of STIM1 and STIM2 proteins, are not dependent on the method of cell reprogramming.