Additional file 1 of Amphibian breeding phenology influences offspring size and response to a common wetland contaminant
Creators: name: Buss, Nicholas
name: Swierk, Lindsey
name: Hua, Jessica
Publisher: name: figshare
Year: 2021
Subjects: subject: Ecology
subject: FOS: Biological sciences

description: Additional file 1: Supplementary Methods and Results. Creation of salt solutions. Collection, analysis and results of temperature data. Table 1. Coordinates for wood frog populations. Table 2. Sample size of wood frog tadpoles that were weighed within each respective breeding cohort (early or late), year (2018 or 2019) and NaCl acclimation treatment (0, 0.5 or 1 g L-1 NaCl) combination. Figure A1. Natal pond conductivity for early vs. late breeding cohorts in 2018 and 2019. Different letters represent significance at p &lt;0.05. Figure A2. Survival curves of tadpoles from seven populations of wood frogs in the early breeding cohort in 2018. The blue line represents tadpole tolerance to 8 g L-1 when not placed in an acclimation treatment. The red line represents tadpole tolerance to 8 g L-1 when reared in 0.5 g L-1 NaCl from the egg to tadpole stage. Figure A3. Survival curves of tadpoles from seven populations of wood frogs in the late breeding cohort in 2018. The blue line represents tadpole tolerance to 8 g L-1 when not placed in an acclimation treatment. The red line represents tadpole tolerance to 8 g L-1 when reared in 0.5 g L-1 NaCl from the egg to tadpole stage. Figure A4. Survival curves of tadpoles from seven populations of wood frogs in the early breeding cohort in 2018. The blue line represents tadpole tolerance to 8 g L-1 when not placed in an acclimation treatment. The red line represents tadpole tolerance to 8 g L-1 when reared in 1 g L-1 NaCl from the egg to tadpole stage. Figure A5. Survival curves of tadpoles from seven populations of wood frogs in the late breeding cohort in 2018. The blue line represents tadpole tolerance to 8 g L-1 when not placed in an acclimation treatment. The red line represents tadpole tolerance to 8 g L-1 when reared in 1 g L-1 NaCl from the egg to tadpole stage. Figure A6. Survival curves of tadpoles from five populations of wood frogs in the early breeding cohort in 2019. The blue line represents tadpole tolerance to 8 g L-1 when not placed in an acclimation treatment. The red line represents tadpole tolerance to 8 g L-1 when reared in 0.5 g L-1 NaCl from the egg to tadpole stage. Figure A7. Survival curves of tadpoles from five populations of wood frogs in the late breeding cohort in 2019. The blue line represents tadpole tolerance to 8 g L-1 when not placed in an acclimation treatment. The red line represents tadpole tolerance to 8 g L-1 when reared in 0.5 g L-1 NaCl from the egg to tadpole stage. Figure A8. Survival curves of tadpoles from five populations of wood frogs in the early breeding cohort in 2019. The blue line represents tadpole tolerance to 8 g L-1 when not placed in an acclimation treatment. The red line represents tadpole tolerance to 8 g L-1 when reared in 1 g L-1 NaCl from the egg to tadpole stage. Figure A9. Survival curves of tadpoles from five populations of wood frogs in the late breeding cohort in 2019. The blue line represents tadpole tolerance to 8 g L-1 when not placed in an acclimation treatment. The red line represents tadpole tolerance to 8 g L-1 when reared in 1 g L-1 NaCl from the egg to tadpole stage. Figure A10. Survival curves of tadpoles from three populations of wood frogs. The blue line represents the tolerance of tadpoles in the early-breeding cohort to NaCl. The red line represents the tolerance of tadpoles in the late-breeding cohort to NaCl.