title: New molecular diagnostic and immunological tools for tuberculosis research
title: Nuovi approci diagnostici ed immunologici per tubercolosi
titleType: AlternativeTitle
Creators: name: CHIACCHIO, TERESA
Publisher: name: Università degli Studi di Roma "Tor Vergata"
Year: 2009
Subjects: subject: tuberculosis; latent TB infection; active TB disease; transrenal DNA; interferon gamma; cytokines; chemokines; RD1 proteins; regulatory T cells; CD39
subject: tuberculosis
subject: latent TB infection
subject: active TB disease
subject: transrenal DNA
subject: interferon gamma
subject: cytokines
subject: chemokines
subject: RD1 proteins
subject: regulatory T cells
subject: CD39

description: 21. ciclo
description: Diagnosis of TB - Active TB disease - Latent TB infection - Pathogenesis of TB - Impact of Treg on suppressing M. tuberculosis-specific response - Aims - Materials and methods 16 First Part: New tools to detect active tuberculosis infection: Mycobacterium tuberculosis DNA in soluble fraction of urine specimens - Study population - Urine specimen preparation - DNA isolation - PCR primer design - PCR amplification and electrophoresis analysis - Second Part: New tools to detect latent tuberculosis infection: evaluation of memory responses and new biomarkers in response to RD1 antigens in TST+ subjects exposed to Mycobacterium tuberculosis - Study population - TST - Whole blood Assay (WBA) based on proteins at day 1 and day 7: Effector and Memory response - Cytokine and chemokine determination - Commercially available assay - Statistical analysis - Third Part: Characterization of regulatory T-cells identified as CD4+CD25highCD39+ in patients with active tuberculosis - Study population - Isolation of peripheral blood mononuclear cells and cultures - Depletion of CD4+CD25+ T-cells 22 Cytokine detection - Antigen-specific response evaluation - Phenotypic analysis - Statistical analysis - Results - First Part: New tools to detect active tuberculosis infection: Mycobacterium tuberculosis DNA in soluble fraction of urine specimens - Second Part: New tools to detect latent tuberculosis infection: evaluation of memory responses and new biomarkers in response to RD1 antigens in TST+ subjects exposed to Mycobacterium tuberculosis - Characteristics of the population - In vitro responses to PPD, RD1 proteins and QF at day 1 post-in vitro stimulation - Memory Response to RD1 proteins - Cytokines different from IFN- that are induced by RD1 proteins - Third Part: Characterization of regulatory T-cells identified as CD4+CD25highCD39+ in patients with active tuberculosis - Epidemiological and demographic characteristics of the subjects enrolled - Phenotypic Analysis of Treg - Characterization of CD39+ T-cells - Ex-vivo and in vitro evaluation of CD4 Treg by different markers in healthy donors and TB patients - Cytokine analysis after RD1-specific stimulation - Depletion of CD4+CD25+CD39+ T-cells from TB patients PBMC increases responses to recall antigens - Discussion - First Part: New tools to detect active tuberculosis infection: Mycobacterium tuberculosis DNA in soluble fraction of urine specimens - Second Part: New tools to detect latent tuberculosis infection: evaluation of memory responses and new biomarkers in response to RD1 antigens in TST+ subjects exposed to Mycobacterium tuberculosis - Third Part: Characterization of regulatory T-cells identified as CD4+CD25highCD39+ in patients with active tuberculosis - References - List of publications