cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
An in vitro coculture approach to study the interplay between dental pulp cells and Streptococcus mutans
Creators: name: Akamp, Tobias
name: Rosendahl, Andreas
name: Galler, Kerstin M.
name: Wölflick, Melanie
name: Buchalla, Wolfgang
name: Widbiller, Matthias
Publisher: name: Universität Regensburg
Year: 2023
Subjects: subject: bacteria, coculture techniques, dental pulp, dentin, glucose, lactic acid
subject: 610 Medizin

description: Aim
To develop a new coculture system that allows exposure of dental pulp cells (DPCs) to Streptococcus mutans and dentine matrix proteins (eDMP) to study cellular interactions in dentine caries.
Methodology
Dental pulp cells and S. mutans were cocultured with or without eDMP for 72 h. Cell proliferation and viability were assessed by cell counting and MTT assays, while bacterial growth and viability were determined by CFU and LIVE/DEAD staining. Glucose catabolism and lactate excretion were measured photometrically as metabolic indicators. To evaluate the inflammatory response, the release of cytokines and growth factors (IL-6, IL-8, TGF-β1, VEGF) was determined by ELISA. Non-parametric statistical analyses were performed to compare all groups and time points (Mann–Whitney U test or Kruskal–Wallis test; α = .05).
Results
While eDMP and especially S. mutans reduced the number and viability of DPCs (p ≤ .0462), neither DPCs nor eDMP affected the growth and viability of S. mutans during coculture (p > .0546). The growth of S. mutans followed a common curve, but the death phase was not reached within 72 h. S. mutans consumed medium glucose in only 30 h, whereas in the absence of S. mutans, cells were able to catabolize glucose throughout 72 h, resulting in the corresponding amount of l-lactate. No change in medium pH was observed. S. mutans induced IL-6 production in DPCs (p ≤ .0011), whereas eDMP had no discernible effect (p > .7509). No significant changes in IL-8 were observed (p > .198). TGF-β1, available from eDMP supplementation, was reduced by DPCs over time. VEGF, on the other hand, was increased in all groups during coculture.
Conclusions
The results show that the coculture of DPCs and S. mutans is possible without functional impairment. The bacterially induced stimulation of proinflammatory and regenerative cytokines provides a basis for future investigations and the elucidation of molecular biological relationships in pulp defence against caries.
title: Fully Integrated Autonomous System-on-Chips with Wireless Energy and Data Transmission
Creators: name: Korner, Dominic
Publisher: name: UNSPECIFIED
Year: 2024

description: Integrated autonomous System-on-Chips (SoCs) with wireless data and energy transmission enable applications in areas that were previously inaccessible due to size constraints or the lack of an energy source. Depending on the transmission medium, the integration of an energy source and communication into the Application-Specific Integrated Circuit ( ASIC ) requires specialized circuitry. Ideally, an autonomous SoC consists of a single ASIC, requiring no external components for operation, making it hereby particularly small.
This work focuses on developing, designing, and fabricating autonomous SoCs with the smallest possible size and as few external components as feasible while still being capable of energy and data transmission. Four different ASICs have been designed and fabricated for three different applications. Two fabricated ASICs use ultrasound for energy and bidirectional data transmission, whereas the other two ASICs use visible light for energy and unidirectional data transmission. The ultrasonic-powered systems presented are a Sensor-integrated Machine element (SiMe) and a medical smart implant. The SiMe can generate a power of 60 mW, providing power to sensors even in fully encapsulated metal enclosures deep inside the machine. The medical smart implant focuses on minimal-sized implants for the human body.
External piezoelectric crystals, required for ultrasound, consume much space compared to the size of an ASIC. Visible light as an energy and data carrier offers the possibility to integrate all components into a single die by using on-chip solar cells. The presented system for discovering novel drug candidates with an ASIC tag uses visible light as an energy and data carrier, and therefore requires no external components. It operates at a high illumination intensity of 10 Mlx, decreasing the overall system size to a minimum. A single solar cell generates voltages of up to 0.7 V, which is not enough to supply the ASIC. Instead of charge pumps, which consume energy and chip area, a serialization of solar cells in a Silicon-on-Insulator (SOI ) technology provides the required supply voltage. To predict the power generated by the solar cell array on the ASIC, different types of solar cells are fabricated and measured. Light encountering the ASIC generates leakage current on the p-n junctions in sensitive circuitry. A countermeasure was taken to reduce the light-induced leakage currents: Measurements show a reduction of the leakage current by over 90 % when using a metal shield and give instructions on how to design it.
This thesis provides design and implementation recommendations for autonomous SoCs with wireless energy and data transmission. It considers light and ultrasound transmission principles for energy and data, as well as provides a system architecture overview for the implementation. The fabricated ASICs prove the effectiveness of the presented recommendations.
Ludioctenus Fairmaire 1893
Creators: name: Kundrаtа, Robin
name: Sormovа, Eliskа
name: Prosvirov, Аlexаnder S.
Publisher: name: Zenodo
Year: 2018
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Coleoptera
Elateridae
Ludioctenus

description: Genus Ludioctenus Fairmaire, 1893
Type species: Ludioctenus akbesianus Fairmaire, 1893; by monotypy [currently synonym of Ludioctenus cyprius (Baudi di Selve, 1871)].
= Elatrigus Reitter, 1905
Type species: Tetrigus cyprius Baudi di Selve, 1871; by monotypy.
description: Published as part of Kundrаtа, Robin, Sormovа, Eliskа & Prosvirov, Аlexаnder S., 2018, Revisiting the diversity of Ludioctenus Fairmaire (Elateridae: Agrypninae), with description of a new species from Afghanistan, and discussion on the systematic position of Tetrigusina, pp. 377-388 in Zootaxa 4455 (2) on page 378, DOI: 10.11646/zootaxa.4455.2.7, http://zenodo.org/record/1457325
Corbidinychus Womersley 1961
Creators: name: Halliday, R. B.
Publisher: name: Zenodo
Year: 2015
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Arachnida
Mesostigmata
Uropodidae
Corbidinychus

description: Corbidinychus Womersley, 1961
Corbidinychus Womersley, 1961a: 107.
Type species Corbidinychus corbicularis Womersley, 1961a: 108, by monotypy.
description: Published as part of Halliday, R. B., 2015, Catalogue of genera and their type species in the mite Suborder Uropodina (Acari: Mesostigmata), pp. 101-147 in Zootaxa 3972 (2) on page 110, DOI: 10.11646/zootaxa.3972.2.1, http://zenodo.org/record/232777
Additional file 1: of Association between trauma and socioeconomic deprivation: a registry-based, Scotland-wide retrospective cohort study of 9,238 patients
Creators: name: Corfield, Alasdair
name: MacKay, Danny
name: Pell, Jill
Publisher: name: Figshare
Year: 2016
Subjects: subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Developmental Biology
subject: Science Policy
subject: Computational Biology

description: Inclusion and exclusion criteria for Scottish Trauma Audit Group. (DOC 47 kb)
Prevalence of genital Mycoplasma in pregnancies with shortened cervix
Creators: name: Rauh, Maximilian
name: Werle, Franziska
name: Schmidt, Börge
name: Litzka, Christian
name: Solano, Maria Emilia
name: Köninger, Angela
Publisher: name: Universität Regensburg
Year: 2023
Subjects: subject: Mycoplasma · Ureaplasma · Cervical insufficiency · Preterm birth
subject: 610 Medizin

description: Objective
To determine whether colonisation with genital Mycoplasma species (spp.) in patients presenting with a shortened cervix before 34th week of pregnancy is associated with preterm birth.
Methods
The collection of this retrospective study consisted of 100 pregnant women who presented to a German Tertiary Perinatal Center between 2017 and 2020 due to a shortened cervix defined as a cervical length of 25 mm or shorter measured by transvaginal ultrasound before 34 weeks of gestation. At the time of admission, gestational age ranged from 18 + 4 to 33 + 3 weeks (+ days) of pregnancy. All patients underwent urine polymerase chain reaction (PCR) for genital Mycoplasma [Ureaplasma (U.) urealyticum, U. parvum, M. hominis or M. genitalium]. Patients who were tested positive underwent a therapy with macrolides (azithromycin or clarithromycin).
Results
37% of the patients were positive for Ureaplasma spp., whereas 5% (5 patients) were Mycoplasma spp.-positive. All the latter were simultaneously colonised with Ureaplasma spp. Ureaplasma-positive patients were significantly younger than those who were tested negative. Median maternal age at examination was 30 years (a) versus 31a (p = 0.04). There was no difference between Ureaplasma-positive and -negative patients regarding median maternal body mass index (BMI) (kg/m2) (23.4 versus 22.3, p = 0.41), cervical length at admission (mm) (15 versus 17, p = 0.17), gestational age at examination (days, d) (198 versus 197, p = 0.97) or gestational age at birth (d) (250 versus 257, p = 0.33), respectively. Comparing U. parvum-positive and U. urealyticum-positive patients, there was some weak indication that U. parvum-positive patients may get a shortening of the cervix earlier in pregnancy, as the median gestational age at examination was 196d versus 215d (p = 0.06). Regarding Mycoplasma-positive and -negative patients, there was no difference in all examined parameters.
Conclusions
Overall, one-third of all women in our study with a shortened cervix before 34th week of pregnancy were colonised with genital Mycoplasma spp. We were able to show that pregnant women, who were treated with antibiotics when tested positive for genital Mycoplasma, gave birth at the same gestational age as patients with a shortened cervix without detected Mycoplasma. This raises the question of whether routine testing and early antibiotic treatment should be established in prenatal care.
Additional file 1: of Total joint arthroplasty: practice variation of physiotherapy across the continuum of care in Alberta
Creators: name: C. Jones
name: Martin, Ruben
name: Westby, Marie
name: Beaupre, Lauren
Publisher: name: Figshare
Year: 2016
Subjects: subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Ecology
subject: FOS: Biological sciences
subject: Science Policy

description: On-line Survey. (PDF 1971Â kb)
The role of growing degree-days in explaining moth species distributions at broad scales
Creators: name: Kharouba, Heather
name: Keefe, Hannah
Publisher: name: Zenodo
Year: 2024
Subjects: growing degree-days
species distribution models
hybrid models
thermal thresholds
Lepidoptera
moths

description: Growing degree-days (GDD), an estimate of an organism's growing season length, has been shown to be an important predictor of Lepidopteran species' distributions and could be influencing Lepidopteran range shifts to climate change. Yet, one understudied simplification in this literature is that the same thermal threshold is used in the calculations of GDD for all species instead of a species-specific threshold. By characterizing the phenological process influenced by climate, a species-specific estimate of GDD should improve the accuracy of species distribution models (SDMs). To test this hypothesis, we use published lab-estimated thermal thresholds and modeled the current geographic distribution of 30 moth species native to North America. We found that the predictive performance of models based on a species-specific estimate of GDD was indistinguishable from models based on a standard estimate of GDD. This is likely because GDD was not an important predictor of these species' distributions. Our findings suggest that lab-estimated thermal thresholds may not always scale up to be predictive at broad scales and that more work is needed to leverage the data from lab experiments into SDMs to accurately predict species' range shifts in response to climate change.
description: Funding provided by: Natural Sciences and Engineering Research CouncilCrossref Funder Registry ID: https://ror.org/01h531d29Award Number:
Coxequesoma Sellnick 1926
Creators: name: Halliday, R. B.
Publisher: name: Zenodo
Year: 2015
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Arachnida
Mesostigmata
Uropodidae
Coxequesoma

description: Coxequesoma Sellnick, 1926
Coxequesoma Sellnick, 1926: 47.
Type species Coxequesoma collegianorum Sellnick, 1926: 47, by original designation.
description: Published as part of Halliday, R. B., 2015, Catalogue of genera and their type species in the mite Suborder Uropodina (Acari: Mesostigmata), pp. 101-147 in Zootaxa 3972 (2) on page 110, DOI: 10.11646/zootaxa.3972.2.1, http://zenodo.org/record/232777
Additional file 1: of Safety and effectiveness evaluation of a domestic peritoneal dialysis fluid packed in non-PVC bags: study protocol for a randomized controlled trial
Creators: name: Jianhui Zhou
name: Xueying Cao
name: Hongli Lin
name: Zhaohui Ni
name: He, Yani
name: Menghua Chen
name: Hongguang Zheng
name: Xiangmei Chen
Publisher: name: Figshare
Year: 2015
Subjects: subject: Medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Cancer
subject: Science Policy

description: The ethical bodies that approved the study in the various centers. This file includes the names of all the ethical bodies who approved the study protocol in every center participating in this clinical trial. (DOC 34 kb)
Crinitodiscus Sellnick 1931
Creators: name: Halliday, R. B.
Publisher: name: Zenodo
Year: 2015
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Arachnida
Mesostigmata
Trachyuropodidae
Crinitodiscus

description: Crinitodiscus Sellnick, 1931
Discopoma (Crinitodiscus) Sellnick, 1931: 721.
Type species Discopoma (Crinitodiscus) beieri Sellnick, 1931: 722, by original designation.
description: Published as part of Halliday, R. B., 2015, Catalogue of genera and their type species in the mite Suborder Uropodina (Acari: Mesostigmata), pp. 101-147 in Zootaxa 3972 (2) on page 110, DOI: 10.11646/zootaxa.3972.2.1, http://zenodo.org/record/232777
Vietnamosasa Nguyen 1990
Creators: name: HAEVERMANS, Thomas
name: NGUYEN, Bich Loan
name: GURGAND, Jacques
name: HAEVERMANS, Agathe
name: DRANSFIELD, Soejatmi
name: DIEP, My Hanh
Publisher: name: Zenodo
Year: 2013
Subjects: Biodiversity
Taxonomy
Plantae
Tracheophyta
Liliopsida
Poales
Poaceae
Vietnamosasa

description: Vietnamosasa Nguyen (1990: 221).
Type:— Vietnamosasa darlacensis T.Q.Nguyen.
description: Published as part of HAEVERMANS, Thomas, NGUYEN, Bich Loan, GURGAND, Jacques, HAEVERMANS, Agathe, DRANSFIELD, Soejatmi & DIEP, My Hanh, 2013, Clearing up Vietnamosasa (Poaceae, Bambusoideae): typification and nomenclature of a distinctive paleotropical bamboo genus, pp. 57-60 in Phytotaxa 137 (1) on page 58, DOI: 10.11646/phytotaxa.137.1.7, http://zenodo.org/record/5086317
Additional file 3: of Identifying the determinants of use of the G&G interventions for older adults in health and social care: protocol of a multilevel approach
Creators: name: Kuiper, Daphne
name: Goedendorp, Martine
name: Sanderman, Robbert
name: Sijmen Reijneveld
name: Nardi Steverink
Publisher: name: Figshare
Year: 2015
Subjects: subject: Medicine
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics
subject: Science Policy
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Factors, time points and number of items per stakeholder level.
Cyclacarus Ewing 1933
Creators: name: Halliday, R. B.
Publisher: name: Zenodo
Year: 2015
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Arachnida
Mesostigmata
Uropodidae
Cyclacarus

description: Cyclacarus Ewing, 1933
Cyclacarus Ewing, 1933: 13.
Type species Cyclacarus aberrans Ewing, 1933: 14, by original designation.
description: Published as part of Halliday, R. B., 2015, Catalogue of genera and their type species in the mite Suborder Uropodina (Acari: Mesostigmata), pp. 101-147 in Zootaxa 3972 (2) on page 110, DOI: 10.11646/zootaxa.3972.2.1, http://zenodo.org/record/232777
Additional file 1: Table S1. of Chronic spinal cord injury attenuates influenza virus-specific antiviral immunity
Creators: name: Bracchi-Ricard, Valerie
name: Zha, Ji
name: Smith, Annalise
name: Darlah Lopez-Rodriguez
name: Bethea, John
name: Andreansky, Samita
Publisher: name: Figshare
Year: 2016
Subjects: subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Biotechnology
subject: Evolutionary Biology
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: Plant Biology
subject: 60506 Virology

description: Primer sequences for qRT PCR. (PDF 146Â kb)
MOESM1 of The impact of a single-nucleotide mutation of bgl2 on cellulase induction in a Trichoderma reesei mutant
Creators: name: Shida, Yosuke
name: Yamaguchi, Kaori
name: Nitta, Mikiko
name: Nakamura, Ayana
name: Takahashi, Machiko
name: Shun-Ichi Kidokoro
name: Mori, Kazuki
name: Tashiro, Kosuke
name: Kuhara, Satoru
name: Matsuzawa, Tomohiko
name: Katsuro Yaoi
name: Sakamoto, Yasumitsu
name: Nobutada Tanaka
name: Morikawa, Yasushi
name: Ogasawara, Wataru
Publisher: name: Figshare
Year: 2015
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Molecular Biology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: Plant Biology

description: Additional file 1: Figure S1. Secreted protein by transformant of PC-3-7 and QM9414. A: Avicel cultivation; 10 µL of the supernatant from day 5 of PC-3-7 series and day 6 of QM9414 series were subjected to SDS-PAGE. Gels were stained by Coomassie Brilliant Blue. B: Cellobiose cultivation; 20 µL of supernatant from day 2.5 of PC-3-7 series and day 3 of QM9414 series were subjected to SDS-PAGE. Gel of Avicel cultivation was stained by Coomassie Brilliant Blue and that of cellobiose cultivation was silver stained.   Figure S2. Gene expression profile of PC-3-7 transformants on Avicel induction. Genes analyzed were cbh1 (A), egl1 (B), bgl2 (C), and xyr1 (D). Stippled gray bar represents PC-3-7, solid gray bar represents PC-Wbgl2, and the shaded gray bar indicates PC-∆bgl2. Values represent the relative expression of each gene normalized to act1 as an internal control. Values represent the means of triplicate experiments. Error bars indicate standard deviations.   Figure S3. Effect of bgl2 mutation and disruption in T. reesei QM9414. A: specific activity of the intracellular cellobiase from transformants QM9414, QM-Mbgl2 and QM-∆bgl2. Cellobiase activity is derived from the mean of triplicate experiments. Error bars indicate standard deviation. B: HPLC analysis of transglycosylation products using cell-free extracts from transformants. Details are as in Fig. 2. Putative transglycosylation products are indicated by arrows.   Figure S4. Southern analysis of transformants for bgl2 analysis. A: the schematic representation of genomic structure of each transformants. B: results of hybridization by gene specific probe for bgl2 and pyr4. Genomic DNA of each transformants was digested by SacI. Lane M represents molecular marker. Table S1. PCR primers used for plasmid construction. Table S2. primers used for real-time quantitative PCR.
Arthrosis diagnosis and treatment recommendations in clinical practice: an exploratory investigation with the generative AI model GPT-4
Creators: name: Pagano, Stefano
name: Holzapfel, Sabrina
name: Kappenschneider, Tobias
name: Meyer, Matthias
name: Maderbacher, Günther
name: Grifka, Joachim
name: Holzapfel, Dominik Emanuel
Publisher: name: Universität Regensburg
Year: 2023
Subjects: subject: Artificial intelligence, ChatGPT-4, Large language model, Orthopaedics, Total joint replacement, Arthrosis
subject: 610 Medizin

description: Background
The spread of artificial intelligence (AI) has led to transformative advancements in diverse sectors, including healthcare. Specifically, generative writing systems have shown potential in various applications, but their effectiveness in clinical settings has been barely investigated. In this context, we evaluated the proficiency of ChatGPT-4 in diagnosing gonarthrosis and coxarthrosis and recommending appropriate treatments compared with orthopaedic specialists.
Methods
A retrospective review was conducted using anonymized medical records of 100 patients previously diagnosed with either knee or hip arthrosis. ChatGPT-4 was employed to analyse these historical records, formulating both a diagnosis and potential treatment suggestions. Subsequently, a comparative analysis was conducted to assess the concordance between the AI’s conclusions and the original clinical decisions made by the physicians.
Results
In diagnostic evaluations, ChatGPT-4 consistently aligned with the conclusions previously drawn by physicians. In terms of treatment recommendations, there was an 83% agreement between the AI and orthopaedic specialists. The therapeutic concordance was verified by the calculation of a Cohen’s Kappa coefficient of 0.580 (p < 0.001). This indicates a moderate-to-good level of agreement. In recommendations pertaining to surgical treatment, the AI demonstrated a sensitivity and specificity of 78% and 80%, respectively. Multivariable logistic regression demonstrated that the variables reduced quality of life (OR 49.97, p < 0.001) and start-up pain (OR 12.54, p = 0.028) have an influence on ChatGPT-4’s recommendation for a surgery.
Conclusion
This study emphasises ChatGPT-4’s notable potential in diagnosing conditions such as gonarthrosis and coxarthrosis and in aligning its treatment recommendations with those of orthopaedic specialists. However, it is crucial to acknowledge that AI tools such as ChatGPT-4 are not meant to replace the nuanced expertise and clinical judgment of seasoned orthopaedic surgeons, particularly in complex decision-making scenarios regarding treatment indications. Due to the exploratory nature of the study, further research with larger patient populations and more complex diagnoses is necessary to validate the findings and explore the broader potential of AI in healthcare.

Level of Evidence: Level III evidence.
Additional file 1: of Identifying the determinants of use of the G&G interventions for older adults in health and social care: protocol of a multilevel approach
Creators: name: Kuiper, Daphne
name: Goedendorp, Martine
name: Sanderman, Robbert
name: Sijmen Reijneveld
name: Nardi Steverink
Publisher: name: Figshare
Year: 2015
Subjects: subject: Medicine
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics

description: Two-step procedure constructing theoretical framework.
Cyllibula Berlese 1916
Creators: name: Halliday, R. B.
Publisher: name: Zenodo
Year: 2015
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Arachnida
Mesostigmata
Trachyuropodidae
Cyllibula

description: Cyllibula Berlese, 1916
Cillibano (Cyllibula) Berlese, 1916a: 23.
Type species Cillibano (Cyllibula) infumata Berlese, 1916a: 23, by original designation.
description: Published as part of Halliday, R. B., 2015, Catalogue of genera and their type species in the mite Suborder Uropodina (Acari: Mesostigmata), pp. 101-147 in Zootaxa 3972 (2) on page 111, DOI: 10.11646/zootaxa.3972.2.1, http://zenodo.org/record/232777
Additional file 1: of Ebola virus disease complicated with viral interstitial pneumonia: a case report
Creators: name: Petrosillo, Nicola
name: Nicastri, Emanuele
name: Lanini, Simone
name: Capobianchi, Maria
name: Caro, Antonino Di
name: Antonini, Mario
name: Puro, Vincenzo
name: Lauria, Francesco
name: Nakono Shindo
name: Magrini, Nicola
name: Kobinger, Gary
name: Ippolito, Giuseppe
Publisher: name: Figshare
Year: 2015
Subjects: subject: Medicine
subject: Molecular Biology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Sociology
subject: FOS: Sociology
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Marine Biology
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: 60506 Virology
subject: Computational Biology

description: Laboratory methods.
Additional file 4: of First do no harm: pain relief for the peripheral venous cannulation of adults, a systematic review and network meta-analysis
Creators: name: Bond, Mary
name: Crathorne, Louise
name: Peters, Jaime
name: Coelho, Helen
name: Haasova, Marcela
name: Cooper, Chris
name: Milner, Quentin
name: Shawyer, Vicki
name: Hyde, Christopher
name: Powell, Roy
Publisher: name: Figshare
Year: 2016
Subjects: subject: Space Science
subject: Medicine
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Science Policy
subject: Computational Biology

description: CONSORT critical appraisal of controlled studies.docx. CONSORT critical appraisal. CONSORT critical appraisal. (DOCX 94Â kb)
Additional file 5: of Microalgal triacylglycerides production in outdoor batch-operated tubular PBRs
Creators: name: Benvenuti, Giulia
name: Rouke Bosma
name: Klok, Anne
name: Ji, Fang
name: Packo Lamers
name: Barbosa, Maria
name: RenĂŠ Wijffels
Publisher: name: Figshare
Year: 2015
Subjects: subject: 29999 Physical Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: Molecular Biology
subject: Evolutionary Biology
subject: FOS: Biological sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: Inorganic Chemistry
subject: FOS: Chemical sciences
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Schematic representation of time-evolution of (time-averaged) TAG productivity.
Identification of DUSP4/6 overexpression as a potential rheostat to NRAS-induced hepatocarcinogenesis
Creators: name: Klemm, Sophie
name: Evert, Katja
name: Utpatel, Kirsten
name: Muggli, Alexandra
name: Simile, Maria M.
name: Chen, Xin
name: Evert, Matthias
name: Calvisi, Diego F.
name: Scheiter, Alexander
Publisher: name: Universität Regensburg
Year: 2023
Subjects: subject: Hepatocellular carcinoma, Cholangiocarcinoma, NRAS, DUSP4, CD133, MAPK pathway
subject: 610 Medizin

description: Background
Upregulation of the mitogen-activated protein kinase (MAPK) cascade is common in hepatocellular carcinoma (HCC). Neuroblastoma RAS viral oncogene homolog (NRAS) is mutated in a small percentage of HCC and is hitherto considered insufficient for hepatocarcinogenesis. We aimed to characterize the process of N-Ras-dependent carcinogenesis in the liver and to identify potential therapeutic vulnerabilities.
Methods
NRAS V12 plasmid was delivered into the mouse liver via hydrodynamic tail vein injection (HTVI). The resulting tumours, preneoplastic lesions, and normal tissue were characterized by NanoString® gene expression analysis, Western Blot, and Immunohistochemistry (IHC). The results were further confirmed by in vitro analyses of HCC cell lines.
Results
HTVI with NRAS V12 plasmid resulted in the gradual formation of preneoplastic and neoplastic lesions in the liver three months post-injection. These lesions mostly showed characteristics of HCC, with some exceptions of spindle cell/ cholangiocellular differentiation. Progressive upregulation of the RAS/RAF/MEK/ERK signalling was detectable in the lesions by Western Blot and IHC. NanoString® gene expression analysis of preneoplastic and tumorous tissue revealed a gradual overexpression of the cancer stem cell marker CD133 and Dual Specificity Phosphatases 4 and 6 (DUSP4/6). In vitro, transfection of HCC cell lines with NRAS V12 plasmid resulted in a coherent upregulation of DUSP4 and DUSP6. Paradoxically, this upregulation in PLC/PRF/5 cells was accompanied by a downregulation of phosphorylated extracellular-signal-regulated kinase (pERK), suggesting an overshooting compensation. Silencing of DUSP4 and DUSP6 increased proliferation in HCC cell lines.
Conclusions
Contrary to prior assumptions, the G12V NRAS mutant form is sufficient to elicit hepatocarcinogenesis in the mouse. Furthermore, the upregulation of the MAPK cascade was paralleled by the overexpression of DUSP4, DUSP6, and CD133 in vivo and in vitro. Therefore, DUSP4 and DUSP6 might fine-tune the excessive MAPK activation, a mechanism that can potentially be harnessed therapeutically.
Additional file 2: Table S2. of Association of nutritional status-related indices and chemotherapy-induced adverse events in gastric cancer patients
Creators: name: Seo, Seung
name: Kim, Sung-Eun
name: Yoon-Koo Kang
name: Baek-Yeol Ryoo
name: Ryu, Min-Hee
name: Jeong, Jae
name: Kang, Shin
name: Mihi Yang
name: Lee, Jung
name: Sung, Mi-Kyung
Publisher: name: Figshare
Year: 2016
Subjects: subject: Space Science
subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: Cancer
subject: Science Policy

description: The relationships of PG-SGA and NRI with diarrhea and abdominal pain. (DOCX 12 kb)
Dendrouropoda Willmann 1959
Creators: name: Halliday, R. B.
Publisher: name: Zenodo
Year: 2015
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Arachnida
Mesostigmata
Uropodidae
Dendrouropoda

description: Dendrouropoda Willmann, 1959
Dendrouropoda Willmann, 1959: 100.
Type species Dendrouropoda schulzi Willmann, 1959: 102, by original designation.
description: Published as part of Halliday, R. B., 2015, Catalogue of genera and their type species in the mite Suborder Uropodina (Acari: Mesostigmata), pp. 101-147 in Zootaxa 3972 (2) on page 111, DOI: 10.11646/zootaxa.3972.2.1, http://zenodo.org/record/232777
Additional file 3: of Ebola virus disease complicated with viral interstitial pneumonia: a case report
Creators: name: Petrosillo, Nicola
name: Nicastri, Emanuele
name: Lanini, Simone
name: Capobianchi, Maria
name: Caro, Antonino Di
name: Antonini, Mario
name: Puro, Vincenzo
name: Lauria, Francesco
name: Nakono Shindo
name: Magrini, Nicola
name: Kobinger, Gary
name: Ippolito, Giuseppe
Publisher: name: Figshare
Year: 2015
Subjects: subject: Medicine
subject: Molecular Biology
subject: Biotechnology
subject: Sociology
subject: FOS: Sociology
subject: Immunology
subject: FOS: Clinical medicine
subject: Marine Biology
subject: Cancer
subject: Science Policy
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: 60506 Virology
subject: FOS: Biological sciences
subject: Computational Biology

description: Person and institution involved in the organization and management of the patient.
Additional file 4: of Microalgal triacylglycerides production in outdoor batch-operated tubular PBRs
Creators: name: Benvenuti, Giulia
name: Rouke Bosma
name: Klok, Anne
name: Ji, Fang
name: Packo Lamers
name: Barbosa, Maria
name: RenĂŠ Wijffels
Publisher: name: Figshare
Year: 2015
Subjects: subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: FOS: Biological sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: Developmental Biology

description: Average culture temperature during the outdoor runs.
Additional file 3: of First do no harm: pain relief for the peripheral venous cannulation of adults, a systematic review and network meta-analysis
Creators: name: Bond, Mary
name: Crathorne, Louise
name: Peters, Jaime
name: Coelho, Helen
name: Haasova, Marcela
name: Cooper, Chris
name: Milner, Quentin
name: Shawyer, Vicki
name: Hyde, Christopher
name: Powell, Roy
Publisher: name: Figshare
Year: 2016
Subjects: subject: Medicine
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Science Policy

description: Study characteristics.docx Study characteristics Characteristics of included studies. (DOCX 87Â kb)
Additional file 1: Table S1. of Continuous evolution of clinical phenotype in 578 Japanese patients with BehĂ§etâ  s disease: a retrospective observational study
Creators: name: Kirino, Yohei
name: Ideguchi, Haruko
name: Takeno, Mitsuhiro
name: Suda, Akiko
name: Higashitani, Kana
name: Kunishita, Yosuke
name: Takase-Minegishi, Kaoru
name: Maasa Tamura
name: Watanabe, Toshiyuki
name: Asami, Yukiko
name: Uehara, Takeaki
name: Yoshimi, Ryusuke
name: Yamazaki, Tetsu
name: Sekiguchi, Akiko
name: Ihata, Atsushi
name: Ohno, Shigeru
name: Ueda, Atsuhisa
name: Toshihisa Igarashi
name: Nagaoka, Shohei
name: Ishigatsubo, Yoshiaki
name: Nakajima, Hideaki
Publisher: name: Figshare
Year: 2016
Subjects: subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics
subject: Developmental Biology
subject: Cancer
subject: Science Policy
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: Computational Biology

description: Phenotypes of patients with special-type BD. (DOC 32 kb)
Additional file 1: Table S1. of Association of nutritional status-related indices and chemotherapy-induced adverse events in gastric cancer patients
Creators: name: Seo, Seung
name: Kim, Sung-Eun
name: Yoon-Koo Kang
name: Baek-Yeol Ryoo
name: Ryu, Min-Hee
name: Jeong, Jae
name: Kang, Shin
name: Mihi Yang
name: Lee, Jung
name: Sung, Mi-Kyung
Publisher: name: Figshare
Year: 2016
Subjects: subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Pharmacology
subject: Biotechnology
subject: Sociology
subject: FOS: Sociology
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics
subject: Marine Biology
subject: Cancer
subject: Science Policy
subject: Hematology

description: Multivariable logistic regression analysis of risk factors for chemotherapy regimen. (DOCX 19 kb)
Characteristics and outcomes of patients hospitalized for infection with influenza, SARS-CoV-2 or respiratory syncytial virus in the season 2022/2023 in a large German primary care centre
Creators: name: Quarg, C.
name: Jörres, R. A.
name: Engelhardt, S.
name: Alter, P.
name: Budweiser, S.
Publisher: name: Universität Regensburg
Year: 2023
Subjects: subject: Hospitalization, PCR test, SARS-CoV-2, RSV, Influenza, Covid-19, Children, Adults, Mortality, ICU admission
subject: 610 Medizin

description: Background
In 2022/2023, Influenza A and Respiratory Syncytial Virus (RSV) reappeared in hospitalized patients, which was in parallel to ongoing SARS-CoV-2 infections. The aim of our study was to compare the characteristics and outcomes of these infections during the same time.
Methods
We included patients of all ages with a positive polymerase chain reaction (PCR) test for Influenza A/B, RSV, or SARS-CoV-2 virus hospitalized in the neurological, internal or paediatric units of the RoMed Hospital Rosenheim, Germany, between October 1st 2022 and February 28th 2023.
Results
A total of 906 patients were included (45.6% female; median age 68.0 years; 21.9% Influenza A, 48.2% SARS-CoV-2, 28.3% RSV). Influenza B (0.2%) and co-infections (1.5%) played a minor role. In patients aged ≥ 18 years (n = 637, 71%), Influenza A, SARS-CoV-2 and RSV groups differed in age (median 72, 79, 76 years, respectively; p < 0.001). Comorbidities, particularly asthma and COPD, were most prevalent for RSV. 103 patients were admitted to the intensive care unit (ICU) (16.3% Influenza A, 15.3% SARS-CoV-2, 19.2% RSV; p = 0.649), 56 died (6.8% Influenza A, 9% SARS-CoV-2, 11.1% RSV; p = 0.496). RSV showed the highest frequencies of low-flow oxygen supplementation for admission and stay. Differences in the length of stay were minor (median 7 days). Conversely, in patients aged < 18 years (n = 261, 28,8%), 19.5%, 17.6% and 60.2% were in the Influenza A, SARS-CoV-2 and RSV groups, respectively; 0.4% showed Influenza B and 2.3% co-infections. 17 patients were admitted to ICU (3.9% Influenza A, 9.6% RSV, 0% SARS-CoV-2); none died. RSV showed the highest frequencies of high- and low-flow oxygen supplementation, SARS-CoV-2 the lowest.
Conclusion
When comparing infections with Influenza, SARS-CoV-2 and RSV in the winter 2022/2023 in hospitalized adult patients, rates of ICU admission and mortality were similar. RSV showed the highest frequencies of obstructive airway diseases, and of oxygen supplementation. The latter was also true in children/adolescents, in whom RSV dominated. Thus, in the situation of declining importance of SARS-CoV-2, RSV showed a disease burden that was relatively higher than that from Influenza and SARS-CoV-2 across ages, and this might be relevant for the seasons coming.
Amelioration of Parkinsonian tremor evoked by DBS: which role play cerebello-(sub)thalamic fiber tracts?
Creators: name: Deuter, Daniel
name: Mederer, Tobias
name: Kohl, Zacharias
name: Forras, Patricia
name: Rosengarth, Katharina
name: Schlabeck, Mona
name: Röhrl, Daniela
name: Wendl, Christina
name: Fellner, Claudia
name: Schmidt, Nils-Ole
name: Schlaier, Jürgen
Publisher: name: Universität Regensburg
Year: 2023
Subjects: subject: DBS · Parkinson’s disease · Movement disorders · Functional network · Probabilistic tractography · Dentatorubro-thalamic tract
subject: 610 Medizin

description: Background
Current pathophysiological models of Parkinson’s disease (PD) assume a malfunctioning network being adjusted by the DBS signal. As various authors showed a main involvement of the cerebellum within this network, cerebello-cerebral fiber tracts are gaining special interest regarding the mediation of DBS effects.
Objectives
The crossing and non-decussating fibers of the dentato-rubro-thalamic tract (c-DRTT/nd-DRTT) and the subthalamo-ponto-cerebellar tract (SPCT) are thought to build up an integrated network enabling a bidimensional communication between the cerebellum and the basal ganglia. The aim of this study was to investigate the influence of these tracts on clinical control of Parkinsonian tremor evoked by DBS.
Methods
We analyzed 120 electrode contacts from a cohort of 14 patients with tremor-dominant or equivalence-type PD having received bilateral STN-DBS. Probabilistic tractography was performed to depict the c-DRTT, nd-DRTT, and SPCT. Distance maps were calculated for the tracts and correlated to clinical tremor control for each electrode pole.
Results
A significant difference between “effective” and “less-effective” contacts was only found for the c-DRTT (p = 0.039), but not for the SPCT, nor the nd-DRTT. In logistic and linear regressions, significant results were also found for the c-DRTT only (pmodel logistic = 0.035, ptract logistic = 0,044; plinear = 0.027).
Conclusions
We found a significant correlation between the distance of the DBS electrode pole to the c-DRTT and the clinical efficacy regarding tremor reduction. The c-DRTT might therefore play a major role in the mechanisms of alleviation of Parkinsonian tremor and could eventually serve as a possible DBS target for tremor-dominant PD in future.
Dentibaiulus Hirschmann 1979
Creators: name: Halliday, R. B.
Publisher: name: Zenodo
Year: 2015
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Arachnida
Mesostigmata
Deraiophoridae
Dentibaiulus

description: Dentibaiulus Hirschmann, 1979
Dentibaiulus Schuster, 1962: 407 (nomen nudum).
Dentibaiulus Hirschmann, 1979b: 61.
Type species Deraiophorus adriaticus Hirschmann & Zirngiebl-Nicol, 1972a: 15, by original designation.
Notes. Schuster (1962) used the name " Dentibaiulus adriaticus (mihi, i. litt.)", but the descriptions of this genus and species were apparently never published. Hirschmann & Zirngiebl-Nicol (1972a) listed the name Dentibaiulus adriaticus Schuster, 1962 as a nomen nudum, and described Schuster's species as Deriaophorus adriaticus Hirschmann & Zirngiebl-Nicol, 1972. Hirschmann (1979b) then made the name Dentibaiulus available for the first time.
description: Published as part of Halliday, R. B., 2015, Catalogue of genera and their type species in the mite Suborder Uropodina (Acari: Mesostigmata), pp. 101-147 in Zootaxa 3972 (2) on page 111, DOI: 10.11646/zootaxa.3972.2.1, http://zenodo.org/record/232777
Additional file 2: of Microalgal triacylglycerides production in outdoor batch-operated tubular PBRs
Creators: name: Benvenuti, Giulia
name: Rouke Bosma
name: Klok, Anne
name: Ji, Fang
name: Packo Lamers
name: Barbosa, Maria
name: RenĂŠ Wijffels
Publisher: name: Figshare
Year: 2015
Subjects: subject: Biochemistry
subject: Space Science
subject: Pharmacology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: Cancer

description: Residual N-NO 3 â   concentration in the medium at the start of the outdoor runs.
Additional file 1: of Microalgal triacylglycerides production in outdoor batch-operated tubular PBRs
Creators: name: Benvenuti, Giulia
name: Rouke Bosma
name: Klok, Anne
name: Ji, Fang
name: Packo Lamers
name: Barbosa, Maria
name: RenĂŠ Wijffels
Publisher: name: Figshare
Year: 2015
Subjects: subject: Evolutionary Biology
subject: FOS: Biological sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: Developmental Biology
subject: Computational Biology

description: Time-evolution of main parameters followed during nitrogen-starvation.
Altered fecal bile acid composition in active ulcerative colitis
Creators: name: Sommersberger, Stefanie
name: Gunawan, Stefan
name: Elger, Tanja
name: Fererberger, Tanja
name: Loibl, Johanna
name: Huss, Muriel
name: Kandulski, Arne
name: Krautbauer, Sabrina
name: Müller, Martina
name: Liebisch, Gerhard
name: Buechler, Christa
name: Tews, Hauke Christian
Publisher: name: Universität Regensburg
Year: 2023
Subjects: subject: Fecal calprotectin, Cholic acid, Deoxycholic acid, Ulcerative Colitis, Crohn´s Disease, Bile acids
subject: 610 Medizin

description: Background
Disturbed bile acid homeostasis associated with a rise of primary and a decline of secondary bile acids is a consistent finding in inflammatory bowel diseases (IBDs). Whether fecal bile acids may emerge as biomarkers for IBD diagnosis and disease severity is less clear. Our study aimed to identify associations of 18 fecal bile acid species with IBD entity and disease activity.
Methods
Stool samples of 62 IBD patients and 17 controls were collected. Eighteen fecal bile acid species were quantified by LC–MS/MS using stable isotope dilution. Lipid levels normalized to a dry weight of the fecal homogenates and ratios of single bile acid species to total bile acid levels were used for calculations.
Results
IBD patients exhibited altered primary and secondary bile acid ratios in stool, with notable distinctions between ulcerative colitis (UC) compared to Crohn’s disease (CD) and healthy controls. Fecal calprotectin was negatively correlated with glycolithocholic acid (GLCA) and hyodeoxycholic acid (HDCA) in UC. These bile acids were reduced in stool of UC patients with fecal calprotectin levels > 500 µg/g compared to UC patients with low calprotectin levels. Moreover, negative associations of six secondary bile acids with C-reactive protein (CRP) existed in UC. In CD patients, fecal bile acids did not correlate with CRP or fecal calprotectin. Diarrhoea is common in IBD, and UC patients with diarrhoea had reduced deoxycholic acid (DCA), glycine conjugated DCA (GDCA) and lithocholic acid in stool in contrast to patients with normal stool consistency. Fecal bile acid levels were not associated with diarrhoea in CD patients. UC patients treated with mesalazine had increased levels of fecal GDCA whereas no such changes were observed in CD patients. Bile acid levels of CD and UC patients treated with biologicals or corticosteroids did not change. Relative levels of GHDCA (specificity: 79%, sensitivity: 67%) and glycochenodeoxycholic acid (specificity: 74%, sensitivity: 63%) were the most specific to distinguish UC from CD.
Conclusion
Disrupted fecal bile acid homeostasis is associated with disease severity and disease symptoms in UC but not in CD, potentially aiding in distinguishing IBD subtypes and classifying the pathophysiology of diarrhoea in UC.
Additional file 5: of First do no harm: pain relief for the peripheral venous cannulation of adults, a systematic review and network meta-analysis
Creators: name: Bond, Mary
name: Crathorne, Louise
name: Peters, Jaime
name: Coelho, Helen
name: Haasova, Marcela
name: Cooper, Chris
name: Milner, Quentin
name: Shawyer, Vicki
name: Hyde, Christopher
name: Powell, Roy
Publisher: name: Figshare
Year: 2016
Subjects: subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Cancer
subject: Science Policy
subject: Computational Biology

description: STROBE crtiical appraisal of surveys.docx. STROBE critical appraisal. STROBE critical appraisal. (DOCX 32Â kb)
Dentidinychus Sellnick 1926
Creators: name: Halliday, R. B.
Publisher: name: Zenodo
Year: 2015
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Arachnida
Mesostigmata
Trematuridae
Dentidinychus

description: Dentidinychus Sellnick, 1926
Dentidinychus Sellnick, 1926: 30.
Type species Dentidinychus zikani Sellnick, 1926: 31, by original designation.
description: Published as part of Halliday, R. B., 2015, Catalogue of genera and their type species in the mite Suborder Uropodina (Acari: Mesostigmata), pp. 101-147 in Zootaxa 3972 (2) on page 111, DOI: 10.11646/zootaxa.3972.2.1, http://zenodo.org/record/232777
Additional file 3: Table S3. of Continuous evolution of clinical phenotype in 578 Japanese patients with BehĂ§etâ  s disease: a retrospective observational study
Creators: name: Kirino, Yohei
name: Ideguchi, Haruko
name: Takeno, Mitsuhiro
name: Suda, Akiko
name: Higashitani, Kana
name: Kunishita, Yosuke
name: Takase-Minegishi, Kaoru
name: Maasa Tamura
name: Watanabe, Toshiyuki
name: Asami, Yukiko
name: Uehara, Takeaki
name: Yoshimi, Ryusuke
name: Yamazaki, Tetsu
name: Sekiguchi, Akiko
name: Ihata, Atsushi
name: Ohno, Shigeru
name: Ueda, Atsuhisa
name: Toshihisa Igarashi
name: Nagaoka, Shohei
name: Ishigatsubo, Yoshiaki
name: Nakajima, Hideaki
Publisher: name: Figshare
Year: 2016
Subjects: subject: Medicine
subject: Evolutionary Biology
subject: FOS: Biological sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Science Policy

description: Rate of therapeutic agents use in groups with different date of onset. (DOC 31 kb)