title: ÐÐ¿ÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð¼ÐµÑÐ¾Ð´ Ð´Ð»Ñ Ð¾ÑÐµÐ½ÐºÐ¸ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÐ¾Ð² Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð¶Ð¸Ð´ÐºÐ¾ÑÑÐµÐ¹
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: An optical method for estimating the parameters of biological fluids, specifically , particle sizes, their electrophoretic mobility and zeta potential
Creators: name: Ð£Ð²Ð°ÑÐ¾Ð²Ð°, ÐÐºÐ°ÑÐµÑÐ¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð¿Ð¾Ð»Ð½Ð¾Ðµ Ð²Ð½ÑÑÑÐµÐ½Ð½ÐµÐµ Ð¾ÑÑÐ°Ð¶ÐµÐ½Ð¸Ðµ
Ð´Ð¸Ð½Ð°Ð¼Ð¸ÑÐµÑÐºÐ¾Ðµ ÑÐ°ÑÑÐµÑÐ½Ð¸Ðµ
ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÑÐ¸ÑÐµÑÐºÐ°Ñ Ð¿Ð¾Ð´Ð²Ð¸Ð¶Ð½Ð¾ÑÑÑ
ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÑÐ¸ÑÐµÑÐºÐ¾Ðµ ÑÐ²ÐµÑÐ¾ÑÐ°ÑÑÐµÑÐ½Ð¸Ðµ
Ð¾Ð¿ÑÐ¸ÑÐµÑÐºÐ¸Ðµ Ð¼ÐµÑÐ¾Ð´Ñ
total internal reflection
dynamic scattering
electrophoretic mobility
electrophoretic light scattering
optical methods

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description: In the work, an analysis of existing and relevant optical methods was carried out and, based on it and the parameters of liquids necessary for consideration, a modified method of electrophoretic light scattering on a prism in the mode of total internal reflection was chosen, which is a combination of the method of electrophoresis and dynamic light scattering. Several experiments were conducted on various liquids: water, an aqueous solution containing 100 nm nanoparticles, and tryptophan.Â  As a result, autocorrelation functions were obtained, with the help of which the parameters of the studied liquids were evaluated. In the course of the work, the accuracy of the method and its advantages were proved, the results obtained indicate its relevance.Â  As a result of the work, a modified optical electrophoretic scattering method was studied and applied, the main advantage of which is the ability to measure particles with a radius in the range from 0.3 nm to several microns. Also, the total internal reflection mode allows you to significantly reduce the volume of the studied liquid. The modified method allows measuring up to 4 parameters simultaneously using the dependence of the studied parameters on the obtained correlation time and the oscillation period of the autocorrelation function. In this work, the radius of the particles, their electrophoretic mobility and zeta potential were measured.