title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ ÑÐ¾ÐµÐ´Ð¸Ð½ÐµÐ½Ð¸Ð¹ ÐºÐ»Ð°ÑÑÐ° Ð´Ð¸ÑÐ¸Ð¾Ð´Ð¸Ð°Ð·Ð¾Ð»Ð¾Ð² Ð½Ð° ÐºÐ°Ð»ÑÑÐ¸ÐµÐ²ÑÑ ÑÐ¸Ð³Ð½Ð°Ð»Ð¸Ð·Ð°ÑÐ¸Ñ Ð² ÐºÐ»ÐµÑÐºÐ°Ñ Ð»Ð¸Ð½Ð¸Ð¸ HEK293
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: The effect of dithiodiazole class compounds on calcium signaling in HEK293 cells
Creators: name: ÐÐ¾Ð²Ð¸ÐºÐ¾Ð²Ð°, Ð®Ð»Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Ð´Ð¸ÑÐ¸Ð°Ð´Ð¸Ð°Ð·Ð¾Ð»Ñ
HEK293
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ÐºÐ°Ð»ÑÑÐ¸ÐµÐ²Ð°Ñ ÑÐ¸Ð³Ð½Ð°Ð»Ð¸Ð·Ð°ÑÐ¸Ñ
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ÑÐ°Ð¿ÑÐ¸Ð³Ð°ÑÐ³Ð¸Ð½-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¹ Ð¾ÑÐ²ÐµÑ
dithiadiazoles
store-operated calcium entry
calcium signaling
modulators
inhibition
tapsigargin-induced response

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«ÐÐ»Ð¸ÑÐ½Ð¸Ðµ ÑÐ¾ÐµÐ´Ð¸Ð½ÐµÐ½Ð¸Ð¹ ÐºÐ»Ð°ÑÑÐ° Ð´Ð¸ÑÐ¸Ð¾Ð´Ð¸Ð°Ð·Ð¾Ð»Ð¾Ð² Ð½Ð° ÐºÐ°Ð»ÑÑÐ¸ÐµÐ²ÑÑ ÑÐ¸Ð³Ð½Ð°Ð»Ð¸Ð·Ð°ÑÐ¸Ñ Ð² ÐºÐ»ÐµÑÐºÐ°Ñ Ð»Ð¸Ð½Ð¸Ð¸ HEK293Â». ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ ÑÐ¾ÐµÐ´Ð¸Ð½ÐµÐ½Ð¸Ð¹ ÐºÐ»Ð°ÑÑÐ° Ð´Ð¸ÑÐ¸Ð°Ð´Ð¸Ð°Ð·Ð¾Ð»Ð¾Ð² Ð½Ð° ÐºÐ°Ð»ÑÑÐ¸ÐµÐ²ÑÑ ÑÐ¸Ð³Ð½Ð°Ð»Ð¸Ð·Ð°ÑÐ¸Ñ Ð² ÐºÐ»ÐµÑÐºÐ°Ñ Ð»Ð¸Ð½Ð¸Ð¸ HEK293. Ð¦ÐµÐ»Ñ ÑÐ°Ð±Ð¾ÑÑ - Ð¿Ð¾Ð¸ÑÐº Ð½Ð¾Ð²ÑÑ Ð¼Ð¾Ð´ÑÐ»ÑÑÐ¾ÑÐ¾Ð² ÐºÐ°Ð»ÑÑÐ¸ÐµÐ²Ð¾Ð³Ð¾ ÑÐ¸Ð³Ð½Ð°Ð»Ð¸Ð½Ð³Ð° ÑÑÐµÐ´Ð¸ Ð²ÐµÑÐµÑÑÐ², Ð¾ÑÐ½Ð¾ÑÑÑÐ¸ÑÑÑ Ðº ÐºÐ»Ð°ÑÑÑ Ð´Ð¸ÑÐ¸Ð°Ð´Ð¸Ð°Ð·Ð¾Ð»Ð¾Ð². Ð ÑÐ¾Ð´Ðµ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð¸Ñ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¸ ÑÐµÑÐµÐ½Ñ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð·Ð°Ð´Ð°ÑÐ¸: 1) ÐÑÐ¾Ð²ÐµÐ´ÐµÐ½ Ð¿ÑÐµÐ´Ð²Ð°ÑÐ¸ÑÐµÐ»ÑÐ½ÑÐ¹ ÑÐºÑÐ¸Ð½Ð¸Ð½Ð³ Ð²ÐµÑÐµÑÑÐ² Ð½Ð° ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ ÑÐ¼ÐµÐ½ÑÑÐ°ÑÑ ÑÐ°Ð¿ÑÐ¸Ð³Ð°ÑÐ³Ð¸Ð½-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¹ Ð¾ÑÐ²ÐµÑ; 2) ÐÐ·ÑÑÐµÐ½Ð° Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾ÑÑÑ Ð¸Ð½Ð´ÐµÐºÑÐ° Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¾Ñ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ Ð´Ð»Ñ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÐ¾ÐµÐ´Ð¸Ð½ÐµÐ½Ð¸Ñ; 3) ÐÐ·ÑÑÐµÐ½Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð¿ÑÐµÐ´Ð²Ð°ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ð¹ Ð¸Ð½ÐºÑÐ±Ð°ÑÐ¸Ð¸ Ñ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ ÑÑÑÐµÐºÑÐ¸Ð²Ð½ÑÐ¼ ÑÐ¾ÐµÐ´Ð¸Ð½ÐµÐ½Ð¸ÐµÐ¼ Ð½Ð° ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ðµ ÐºÐ°Ð»ÑÑÐ¸Ñ Ð²Ð¾ Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð´ÐµÐ¿Ð¾, Ð½Ð° ÑÑÐ¾Ð²ÐµÐ½Ñ ÑÐ°Ð¿ÑÐ¸Ð³Ð°ÑÐ³Ð¸Ð½-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ ÐºÐ°Ð»ÑÑÐ¸ÐµÐ²Ð¾Ð³Ð¾ Ð²ÑÐ±ÑÐ¾ÑÐ° Ð¸ Ð°Ð¼Ð¿Ð»Ð¸ÑÑÐ´Ñ Ð´ÐµÐ¿Ð¾-ÑÐ¿ÑÐ°Ð²Ð»ÑÐµÐ¼Ð¾Ð³Ð¾ ÐºÐ°Ð»ÑÑÐ¸ÐµÐ²Ð¾Ð³Ð¾ Ð²ÑÐ¾Ð´Ð°; 4) ÐÑÐµÐ½ÐµÐ½Ð° ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÐ¾ÐµÐ´Ð¸Ð½ÐµÐ½Ð¸Ñ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾Ð²Ð°ÑÑ Ð´ÐµÐ¿Ð¾-ÑÐ¿ÑÐ°Ð²Ð»ÑÐµÐ¼ÑÐ¹ Ð²ÑÐ¾Ð´ ÐºÐ°Ð»ÑÑÐ¸Ñ Ñ ÑÐ¶Ðµ Ð°ÐºÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¼Ð¸ Ð´ÐµÐ¿Ð¾-ÑÐ¿ÑÐ°Ð²Ð»ÑÐµÐ¼ÑÐ¼Ð¸ ÐºÐ°Ð½Ð°Ð»Ð°Ð¼Ð¸. ÐÑÐµ Ð´Ð°Ð½Ð½ÑÐµ Ð¿Ð¾Ð»ÑÑÐµÐ½Ñ Ð½Ð° Ð±Ð°Ð·Ðµ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ Ð¸Ð¾Ð½Ð½ÑÑ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð¼ÐµÐ¼Ð±ÑÐ°Ð½ Ð² Ð¾ÑÐ´ÐµÐ»Ðµ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾Ð¹ ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ð¸ ÐºÐ»ÐµÑÐºÐ¸ ÐÐ½ÑÑÐ¸ÑÑÑÐ° ÑÐ¸ÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð ÐÐ. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð¿ÑÐµÐ´Ð²Ð°ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ ÑÐµÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¾ Ð½Ð°Ð¹Ð´ÐµÐ½Ð¾ Ð²ÐµÑÐµÑÑÐ²Ð¾, ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾Ðµ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾Ð²Ð°ÑÑ ÑÐ°Ð¿ÑÐ¸Ð³Ð°ÑÐ³Ð¸Ð½-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¹ ÐºÐ°Ð»ÑÑÐ¸ÐµÐ²ÑÐ¹ Ð¾ÑÐ²ÐµÑ, Ð´Ð°Ð»ÐµÐµ Ð¼Ñ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð»Ð¸, ÑÑÐ¾ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð¿ÑÐ¾Ð¸ÑÑÐ¾Ð´Ð¸Ñ Ð½Ðµ ÑÐ¾Ð»ÑÐºÐ¾ Ð¿ÑÐ¸Â Ð¿ÑÐµÐ´Ð²Ð°ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ð¹ Ð¸Ð½ÐºÑÐ±Ð°ÑÐ¸Ð¸ Ñ Ð´Ð°Ð½Ð½ÑÐ¼ Ð²ÐµÑÐµÑÑÐ²Ð¾Ð¼, Ð½Ð¾ Ð² ÑÐ°Ðº Ð½Ð°Ð·ÑÐ²Ð°ÐµÐ¼Ð¾Ð¼ Â«Ð¾ÑÑÑÐ¾Ð¼ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐµÂ». ÐÑ Ð¿ÑÐµÐ´Ð¿Ð¾Ð»Ð°Ð³Ð°ÐµÐ¼, ÑÑÐ¾ Ð² Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐµÐ¼ Ð´Ð°Ð½Ð½Ð¾Ðµ ÑÐ¾ÐµÐ´Ð¸Ð½ÐµÐ½Ð¸Ðµ Ð¼Ð¾Ð¶ÐµÑ Ð±ÑÑÑ ÑÐ°ÑÑÐ¼Ð¾ÑÑÐµÐ½Ð¾ ÐºÐ°Ðº Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»ÑÐ½Ð¾Ðµ Ð»ÐµÐºÐ°ÑÑÑÐ²ÐµÐ½Ð½Ð¾Ðµ ÑÑÐµÐ´ÑÑÐ²Ð¾ Ð¸Ð»Ð¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°ÑÑÑÑ Ð² Ð½Ð°ÑÑÐ½Ð¾Ð¹ Ð¿ÑÐ°ÐºÑÐ¸ÐºÐµ ÐºÐ°Ðº Ð¼Ð¾Ð´ÑÐ»ÑÑÐ¾Ñ ÐºÐ°Ð»ÑÑÐ¸ÐµÐ²Ð¾Ð¹ ÑÐ¸Ð³Ð½Ð°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸.
description: The subject of the graduate qualification work is âThe effect of dithiodiazole class compounds on calcium signaling in HEK293 cellsâ. The given work is devoted to the study of the effect of the ditiadiazole class compounds on calcium signaling in HEK293 cells. The main goal of the project was to find new calcium signaling modulators among substances belonging to the class of dithiadiazoles.As a result of the work, the following tasks were solved: 1) Preliminary screening of compounds for the ability to reduce the tapsigargin-induced calcium response was carried out; 2) The dependence of the inhibition index on the concentration for the most effective compound was studied; 3) The effects of preincubation with the most effective compound were studied, including calcium concentration in intracellular storage, the level of tapsigargin-induced calcium release and the amplitude of store-operated calcium entry; 4) The ability of the most effective compound to inhibit activated store-operated calcium channels was evaluated.All data were obtained in the laboratory of ion channels of cell membranes of the molecular physiology of the cell department of the Institute of Cytology of the Russian Academy of Sciences. As a result of preliminary testing, a compound capable of inhibiting the tapsigargin-induced calcium response was found, further experiments confirmed this ability, and it was shown that inhibition occurs not only during preincubation with this compound but also in the so-called "acute experiment". We assume that in the future this compound can be considered as a potential drug or used in scientific practice as a calcium signaling modulator.