title: ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ñ ÑÐ¾Ð¼Ð°Ð½ÑÐ¸Ð½-ÑÐµÐ¼Ð¾Ð´ÐµÐ»Ð¸ÑÑÑÑÐµÐ³Ð¾ ÑÐ°ÐºÑÐ¾ÑÐ° CHD1 Ð¸ Ð°ÑÐµÑÐ¸Ð»ÑÑÐ°Ð½ÑÑÐµÑÐ°Ð·Ñ MOF
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Investigation of the interaction of the chromatin-remodeling factor CHD1 and acetyltransferase MOF
Creators: name: Ð ÑÐ±Ð°Ð»ÐºÐ¸Ð½Ð°, ÐÐ½Ð°ÑÑÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Drosophila melanogaster
Ð´Ð¾Ð·Ð¾Ð²Ð°Ñ ÐºÐ¾Ð¼Ð¿ÐµÐ½ÑÐ°ÑÐ¸Ñ
CHD1
Ð°ÑÐµÑÐ¸Ð»ÑÑÐ°Ð½ÑÑÐµÑÐ°Ð·Ð° MOF
Ð¿Ð¾Ð»Ð¸ÑÐµÐ½Ð½ÑÐµ Ñ ÑÐ¾Ð¼Ð¾ÑÐ¾Ð¼Ñ
Ð¸Ð¼Ð¼ÑÐ½Ð¾Ð±Ð»Ð¾ÑÑÐ¸Ð½Ð³
dose compensation
MOF acetyltransferase
polytene chromosomes
immunoblotting

description: Ð¦ÐµÐ»ÑÑ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÐµÑÑÑ Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ ÑÐ²ÐµÑÑ-ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ ÑÐ°Ðº-ÑÐ¾ÑÐ° CHD1 Ð½Ð° Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ñ Ð¥-ÑÑÐ¾Ð¼Ð¾ÑÐ¾Ð¼Ñ ÑÐ°Ð¼ÑÐ¾Ð² Ð´ÑÐ¾Ð·Ð¾ÑÐ¸Ð»Ñ Ð¸ Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ñ Ð°ÑÐµÑÐ¸Ð»ÑÑÐ°Ð½ÑÑÐµÑÐ°Ð·Ñ MOF, Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ ÑÐ²ÐµÑÑ-ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð°ÑÐµÑÐ¸Ð»ÑÑÐ°Ð½ÑÑÐµ-ÑÐ°Ð·Ñ MOF Ð½Ð° Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ñ Ð¥-ÑÑÐ¾Ð¼Ð¾ÑÐ¾Ð¼Ñ ÑÐ°Ð¼ÑÐ¾Ð² Ð´ÑÐ¾Ð·Ð¾ÑÐ¸Ð»Ñ Ð¸ Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ñ ÑÐ°ÐºÑÐ¾ÑÐ° CHD1, Ð²Ð·Ð°Ð¸Ð¼Ð½Ð¾Ð¹ Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ CHD1 Ñ Ð°ÑÐµÑÐ¸Ð»ÑÑÐ°Ð½ÑÑÐµÑÐ°Ð·Ð¾Ð¹ MOF, Ð° ÑÐ°ÐºÐ¶Ðµ ÑÐ¾ÑÑÐ°Ð²Ð»ÐµÐ½Ð¸Ðµ Ð¿ÑÐ¾ÑÐ¾ÐºÐ¾Ð»Ð° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ Ð¸Ð¼Ð¼ÑÐ½Ð¾Ð±Ð»Ð¾ÑÑÐ¸Ð½Ð³Ð° Ð´Ð»Ñ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¸Ñ Ð±ÐµÐ»ÐºÐ¾Ð² CHD1 Ð¸ MOF. ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð¸Ñ Ð²ÑÐ¿ÑÑÐº-Ð½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: 1) Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÑ Ð¾ÑÐµÑÐµÑÑÐ²ÐµÐ½Ð½ÑÑ Ð¸ Ð·Ð°ÑÑÐ±ÐµÐ¶Ð½ÑÑ Ð¸ÑÑÐ¾ÑÐ½Ð¸ÐºÐ¾Ð² Ð¿Ð¾ Ð²Ð¾Ð¿ÑÐ¾ÑÐ°Ð¼: Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð° ÑÐ°Ð±Ð¾ÑÑ Ð´Ð¾Ð·Ð¾Ð²Ð¾Ð¹ ÐºÐ¾Ð¼Ð¿ÐµÐ½ÑÐ°ÑÐ¸Ð¸ Ñ Drosophila melanogaster, ÑÑÐ¾Ð¼Ð°ÑÐ¸Ð½-ÑÐµÐ¼Ð¾Ð´ÐµÐ»Ð¸ÑÑÑÑÐµÐ³Ð¾ ÑÐ°ÐºÑÐ¾ÑÐ° CHD1 Ð¸ Ð°ÑÐµÑÐ¸Ð»ÑÑÐ°Ð½ÑÑÐµÑÐ°Ð·Ñ MOF; 2) Ð¾ÑÑÐ°Ð±Ð¾ÑÐºÐ° ÑÑÐ»Ð¾Ð²Ð¸Ð¹ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ Ð¸Ð¼Ð¼ÑÐ½Ð¾Ð±Ð»Ð¾ÑÑÐ¸Ð½Ð³Ð° Ð½Ð° Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¸Ðµ Ð¸ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ðµ Ð±ÐµÐ»ÐºÐ¾Ð² CHD1 Ð¸ MOF; 3) Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ðµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð² Ð¸Ð¼Ð¼ÑÐ½Ð¾Ð³Ð¸ÑÑÐ¾ÑÐ¸Ð¼Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð°, Ð¾ÑÑÐ°Ð¶Ð°Ñ-ÑÐ¸Ñ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¥-ÑÑÐ¾Ð¼Ð¾ÑÐ¾Ð¼Ñ ÑÐ°Ð¼ÑÐ¾Ð² Ð¸ Ð¿ÑÐ¸Ð²Ð»ÐµÑÐµÐ½Ð¸Ñ Ð±ÐµÐ»ÐºÐ° CHD1 Ðº Ð½ÐµÐ¹ Ð¿ÑÐ¸ Ð¿Ð¾Ð²ÑÑÐµÐ½Ð½Ð¾Ð¹ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ ÑÑÐ¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ°; 4) Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ðµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð² Ð¸Ð¼Ð¼ÑÐ½Ð¾Ð³Ð¸ÑÑÐ¾ÑÐ¸Ð¼Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð°, Ð¾ÑÑÐ°Ð¶Ð°Ñ-ÑÐµÐ³Ð¾ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¥-ÑÑÐ¾Ð¼Ð¾ÑÐ¾Ð¼Ñ ÑÐ°Ð¼ÑÐ¾Ð² Ð¸ Ð¿ÑÐ¸Ð²Ð»ÐµÑÐµÐ½Ð¸Ðµ Ð±ÐµÐ»ÐºÐ° CHD1 Ðº Ð½ÐµÐ¹ Ð¿ÑÐ¸ Ð¿Ð¾Ð²ÑÑÐµÐ½Ð½Ð¾Ð¹ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð±ÐµÐ»ÐºÐ° MOF; 5) Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ðµ Ð´Ð°Ð½Ð½ÑÑ Ð¿Ð¾ Ð²Ð·Ð°Ð¸Ð¼Ð½Ð¾Ð¹ Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ ÑÐ°ÐºÑÐ¾ÑÐ° ÑÐ±Ð¾ÑÐºÐ¸ Ð¸ ÑÐµ-Ð¼Ð¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÑÑÐ¾Ð¼Ð°ÑÐ¸Ð½Ð° CHD1 Ð¸ Ð°ÑÐµÑÐ¸Ð»ÑÑÐ°Ð½ÑÑÐµÑÐ°Ð·Ñ MOF Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð½ÐµÐ¿ÑÑ-Ð¼Ð¾Ð¹ Ð¸Ð¼Ð¼ÑÐ½Ð¾ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸. ÐÑÐ¸ Ð½Ð°Ð¿Ð¸ÑÐ°Ð½Ð¸Ð¸ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±Ð·Ð¾ÑÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð±Ð°Ð·ÑÂ  Ð´Ð°Ð½Ð½ÑÑ: ÐÐºÐ°Ð´ÐµÐ¼Ð¸Ñ Google, eLIBRARY, PubMed.Â  ÐÐ°ÑÐµÑÑÐ²ÐµÐ½Ð½ÑÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð² Ð²Ð¸Ð´Ðµ ÑÐ»ÑÐ¾ÑÐµÑ-ÑÐµÐ½ÑÐ½ÑÑ Ð¼Ð¸ÐºÑÐ¾ÑÐ¾ÑÐ¾Ð³ÑÐ°ÑÐ¸Ð¹, Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»Ð¸ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ñ Ado-be Photoshop. Ð ÑÐ¾Ð´Ðµ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ñ Ð¿Ð°ÑÑÐµÑÐ½Ñ Ð¿Ð¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ ÑÐ°ÐºÑÐ¾ÑÐ° ÑÐ±Ð¾ÑÐºÐ¸ Ð¸ ÑÐµÐ¼Ð¾Ð´ÐµÐ»Ð¸ÑÐ²Ð¾Ð°Ð½Ð¸Ñ ÑÑÐ¾Ð¼Ð°ÑÐ¸Ð½Ð° CHD1 Ð¸ Ð°ÑÐµÑÐ¸Ð»ÑÑÑÐ°Ð½ÑÑÐµÑÐ°Ð·Ñ MOF, ÐºÐ¾Ð¼Ð¿Ð¾Ð½ÐµÐ½ÑÐ° ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ° Ð´Ð¾Ð·Ð¾Ð²Ð¾Ð¹ ÐºÐ¾Ð¼Ð¿ÐµÐ½ÑÐ°ÑÐ¸Ð¸, Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ Ð½Ð¾ÑÐ¼Ð°Ð»ÑÐ½Ð¾Ð¹ Ð¸ Ð¿Ð¾Ð²ÑÑÐµÐ½Ð½Ð¾Ð¹ Ð¸Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ñ ÑÐ°Ð¼ÑÐ¾Ð² Drosophila melanogaster.Â  ÐÑÐ»Ð¾ ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¾, ÑÑÐ¾ Ñ Ð¾ÑÐ¾Ð±ÐµÐ¹ Ð´Ð¸ÐºÐ¾Ð³Ð¾ ÑÐ¸Ð¿Ð° Ð¿ÑÐ¸ Ð½Ð¾ÑÐ¼Ð°Ð»ÑÐ½Ð¾Ð¹ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ CHD1 Ð¸ MOF ÑÐ²ÑÐ·ÑÐ²Ð°ÑÑÑÑ ÑÐ¾ ÑÐ¿ÐµÑÐ¸-ÑÐ¸ÑÐ½ÑÐ¼Ð¸ Ð´Ð»Ñ Ð½Ð¸Ñ ÑÐ°Ð¹ÑÐ°Ð¼Ð¸ ÑÐ²ÑÐ·ÑÐ²Ð°Ð½Ð¸Ñ Ð½Ð° Ð¥-ÑÑÐ¾Ð¼Ð¾ÑÐ¾Ð¼Ðµ ÑÐ°Ð¼ÑÐ° Ð¸ Ð² Ð°ÑÑÐ¾ÑÐ¾Ð¼Ð°Ñ (CHD1). ÐÐ´Ð½Ð°ÐºÐ¾ Ð¿ÑÐ¸ ÑÐ²ÐµÑÑ-ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ CHD1 Ð°ÑÐµÑÐ¸Ð»ÑÑÐ°Ð½ÑÑÐµÑÐ°Ð·Ð° MOF ÑÐµÐºÑÑ-ÑÐ¸ÑÑÐµÑÑÑ Ð² ÑÐºÑÐ¾Ð¿Ð¸ÑÐµÑÐºÐ¸Ðµ ÑÐ°Ð¹ÑÑ Ð½Ð° Ð°ÑÑÐ¾ÑÐ¾Ð¼Ð°Ñ, ÑÑÑÑÐºÑÑÑÐ° Ð¥-ÑÑÐ¾Ð¼Ð¾ÑÐ¾Ð¼ ÑÐ¸Ð»ÑÐ½Ð¾ Ð¸Ð·Ð¼ÐµÐ½ÑÐµÑÑÑ: Ð¾Ð½Ð¸ ÑÑÐ°Ð½Ð¾Ð²ÑÑÑÑ Ð´ÐµÐºÐ¾Ð½Ð´ÐµÐ½ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¼Ð¸ Ð² ÑÐ°Ð¹Ð¾Ð½Ð°Ñ ÑÐ²ÑÐ·ÑÐ²Ð°Ð½Ð¸Ñ Ñ CHD1, Ð¿ÑÐ¸Ð²Ð»ÐµÑÐµÐ½Ð¸Ðµ MOF Ð½Ð°ÑÑÑÐ°ÐµÑÑÑ Ð² Ð´ÐµÐºÐ¾Ð½Ð´ÐµÐ½ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ ÑÐ°Ð¹Ð¾Ð½Ð°Ñ ÑÑÐ¾Ð¼Ð¾-ÑÐ¾Ð¼, Ð° Ð¿ÑÐ¸ ÑÐ²ÐµÑÑ-ÑÐºÑÐ¿ÑÐµÑÑÐ¸ MOF Ð½Ð°Ð±Ð»ÑÐ´Ð°ÐµÑÑÑ Ð¾Ð±ÑÐ°ÑÐ½ÑÐ¹ ÑÑÑÐµÐºÑ: MOF Ð²ÑÑÐµÑ-Ð½ÑÐµÑ CHD1 Ñ Ð¥-ÑÑÐ¾Ð¼Ð¾ÑÐ¾Ð¼Ñ, Ð¸ Ð¿ÑÐ¾Ð¸ÑÑÐ¾Ð´Ð¸Ñ Ð³Ð¸Ð¿ÐµÑ-Ð°ÑÐµÑÐ¸Ð»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð²ÑÐµÑ ÑÑÐ¾Ð¼Ð¾-ÑÐ¾Ð¼, Ð¿ÑÐ¸ ÑÑÐ¾Ð¼ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ñ Ð¥-ÑÑÐ¾Ð¼Ð¾ÑÐ¾Ð¼Ñ Ð½Ðµ Ð¾ÑÐ»Ð¸ÑÐ°ÐµÑÑÑ Ð¾Ñ ÑÑÑÐ¾ÐµÐ½Ð¸Ñ Ð¥-ÑÑÐ¾Ð¼Ð¾ÑÐ¾Ð¼ Ð¾ÑÐ¾Ð±ÐµÐ¹ Ð´Ð¸ÐºÐ¾Ð³Ð¾ ÑÐ¸Ð¿Ð°. ÐÑÑÐ²Ð»ÐµÐ½Ð¾, ÑÑÐ¾ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ Ð² ÑÑÐ¾Ð²Ð½Ðµ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð±ÐµÐ»ÐºÐ° CHD1 Ð¿ÑÐµÐ¿ÑÑÑÑÐ²ÑÐµÑ ÑÐ¾ÑÐ½Ð¾Ð¼Ñ Ð½Ð°ÑÐµÐ»Ð¸Ð²Ð°Ð½Ð¸Ñ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ° Ð´Ð¾Ð·Ð¾Ð²Ð¾Ð¹ ÐºÐ¾Ð¼Ð¿ÐµÐ½-ÑÐ°ÑÐ¸Ð¸ Ð½Ð° Ð¥-ÑÑÐ¾Ð¼Ð¾ÑÐ¾Ð¼Ñ ÑÐ°Ð¼ÑÐ¾Ð² Ð´ÑÐ¾Ð·Ð¾ÑÐ¸Ð»Ñ. Ð¢Ð°ÐºÐ¶Ðµ Ð±ÑÐ»Ð¾ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°-Ð½Ð¸Ðµ Ð¿Ð¾ Ð´ÐµÑÐµÐºÑÐ¸Ð¸ Ð±ÐµÐ»ÐºÐ¾Ð² CHD1 Ð¸ MOF Ð² Ð±ÐµÐ»ÐºÐ¾Ð²Ð¾Ð¼ ÑÐºÑÑÑÐ°ÐºÑÐµ, Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½Ð¾Ð¼ Ð¸Ð· ÑÐ¼Ð±ÑÐ¸Ð¾Ð½Ð¾Ð² Ð¼ÑÑ Ð´Ð¸ÐºÐ¾Ð³Ð¾ ÑÐ¸Ð¿Ð°, Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð¸Ð¼Ð¼ÑÐ½Ð¾Ð±Ð»Ð¾ÑÑÐ¸Ð½Ð³Ð°. Ð ÑÐ¾Ð´Ðµ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¾ ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¾ Ð·Ð°ÑÑÑÐ´Ð½Ð¸ÑÐµÐ»ÑÐ½Ð¾Ðµ Ð²ÑÑÐ²Ð»ÐµÐ½Ð¸Ðµ Ð±ÐµÐ»ÐºÐ¾Ð² CHD1 Ð¸ MOF Ð¾Ð´Ð½Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾ Ð½Ð° Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ðµ Ð¿ÑÐ¸ ÑÐµÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð¿Ð¾Ð»ÑÑÑÑÐ¾Ð³Ð¾ Ð¸ Ð¼Ð¾ÐºÑÐ¾Ð³Ð¾ Ð¿ÐµÑÐµÐ½Ð¾ÑÐ° Ñ ÐÐÐÐ Ð¿Ð¾ Ð¿ÑÐ¸-ÑÐ¸Ð½Ðµ Ð²ÑÑÐ¾ÐºÐ¾Ð³Ð¾ Ð¸ Ð½Ð¸Ð·ÐºÐ¾Ð³Ð¾ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾Ð³Ð¾ Ð²ÐµÑÐ° Ð±ÐµÐ»ÐºÐ°, Ð´Ð»Ñ CHD1 Ð¸ MOF ÑÐ¾Ð¾Ñ-Ð²ÐµÑÑÑÐ²ÐµÐ½Ð½Ð¾. Ð Ð°Ð±Ð¾ÑÐ° Ð±ÑÐ»Ð° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ ÐÐ°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ Ð­ÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½Ð¾Ð¹ ÐÐµÐ½ÐµÑÐ¸-ÐºÐ¸ ÐÐÐ Ð Â«ÐÐÐ¦ ÐÑÑÑÐ°ÑÐ¾Ð²ÑÐºÐ¸Ð¹ Ð¸Ð½ÑÑÐ¸ÑÑÑ â ÐÐÐ¯Ð¤Â».
description: The aim of this work is to study the effect of CHD1 or MOF overexpression on the morphology of the X chromosome in Drosophila males, the mutual localization of CHD1 with MOF acetyltransferase, and to develop a protocol for immunoblotting to detect CHD1 and MOF proteins. Tasks that were solved during the final qualifica-tion work: 1) studying the literature of domestic and foreign sources on the following is-sues: the mechanism of dose compensation in Drosophila melanogaster, chromatin-remodeling factor CHD1 and acetyltransferase MOF; 2) development of the conditions for conducting immunoblotting for the detec-tion and interaction of CHD1 and MOF proteins; 3) obtaining the results of immunohistochemical analysis, reflecting the change in the morphology of the X chromosome of males and the attraction of the CHD1 protein to it with an increased expression of this protein; 4) obtaining the results of immunohistochemical analysis, reflecting the change in the morphology of the X chromosome of males and the attraction of the CHD1 protein to it with an increased expression of MOF; 5) obtaining data on the mutual localization of the chromatin assembly and re-modeling factor CHD1 and acetyltransferase MOF by indirect immunofluorescence. When writing the literature review, the following databases were used: Google Academy, eLIBRARY, PubMed. The experimental data obtained in the form of fluorescent micrographs were qualitative processed using the Adobe Photoshop program. In the course of the work, the patterns of behavior of the chromatin assembly areÂ  remodeling factor CHD1 and MOF acetyltransferase, a component of the dose com-pensation complex, were studied under conditions of their normal and increased ex-pression in Drosophila melanogaster males. It was found that in wild-type individu-als with normal expression, CHD1 and MOF bind to their specific binding sites on the male X chromosome and in autosom regions (CHD1). However, when CHD1 is overexpressed, MOF acetyltransferase is recruited to ectopic sites on autosomes, the structure of X chromosomes changes dramatically: they become decondensed, MOF disappears from the decondensed regions, and when MOF is overexpressed, the op-posite effect is observed: MOF displaces CHD1 from the X chromosome , and hy-per-acetylation of all chromosomes occurs, while the morphology of the X chromo-some does not differ from the structure of the X chromosomes of wild-type individ-uals. It was found that changes in the level of expression of CHD1 and MOF pro-teins prevent the precise targeting of the dosage compensation complex to the X chromosome of Drosophila males. A study was also carried out on the detection of CHD1 and MOF proteins in a protein extract isolated from wild-type fly embryos by immunoblotting. In the course of the work, difficult migration of CHD1 and MOF proteins from PAAG to the membrane during semi-dry and wet transfer was estab-lished due to the high and low molecular weight of the protein for CHD1 and MOF, respectively. The work was performed based on the Laboratory of Experimental Genetics of the OMRB "NRC Kurchatov Institute â PNPI".