title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ ÑÑÐ»Ð¾Ð²Ð¸Ð¹ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Pseudomonas aÐµruginosa Ð½Ð° Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° Ð±Ð¸Ð¾Ð¼ÐµÐ¼Ð±ÑÐ°Ð½
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: The influence of the culturing conditions of Pseudomonas aeruginosa on the morphological properties of biomembranes
Creators: name: ÐÑÑÐ¸Ð½, ÐÐ¼Ð¸ÑÑÐ¸Ð¹
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Ð±Ð¸Ð¾Ð¿Ð»ÐµÐ½ÐºÐ¸
pseudomonas aeruginosa
96-Ð»ÑÐ½Ð¾ÑÐ½ÑÐ¹ Ð¿Ð»Ð°Ð½ÑÐµÑ
Ð°Ð²ÑÐ¾Ð¼Ð°ÑÐ¸ÑÐµÑÐºÐ¾Ðµ ÑÐ¾ÑÐ¾Ð¼ÐµÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
Ð±Ð¸Ð¾Ð¼Ð°ÑÑÐ° Ð±Ð¸Ð¾Ð¿Ð»ÐµÐ½Ð¾ÑÐ½ÑÑ ÑÑÑÑÐºÑÑÑ
biofilms
96-well plate
automatic photometry
bio-mass of biofilm structures

description: ÐÐ°Ð´Ð°ÑÐ¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ ÑÐ¾ÑÑÐ° Ð¸ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ñ Ð±Ð¸Ð¾Ð¿Ð»ÐµÐ½Ð¾ÑÐ½ÑÑ ÑÑÑÑÐºÑÑÑ, Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð½ÑÑ Ð¼Ð¸ÐºÑÐ¾Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð¾Ð¼ P. Ð°eruginosa Ð¿ÑÐ¸ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ ÑÑÐ»Ð¾Ð²Ð¸ÑÑ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ, Ð¿ÑÐµÐ¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð´Ð°Ð½Ð½ÑÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð´Ð»Ñ ÑÐ°ÑÑÐµÑÐ° Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð±Ð¸Ð¾Ð¿Ð»ÐµÐ½Ð¾Ðº. ÐÐ±ÑÐµÐºÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â ÑÑ. P. Ð°eruginosa 7681, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÐ¹ Ð¸Ð· Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ð° Ð¿Ð°ÑÐ¸ÐµÐ½ÑÐ° Ð¡ÐÐ± ÐÐÐ£Ð "ÐÐ»Ð¸Ð·Ð°Ð²ÐµÑÐ¸Ð½ÑÐºÐ°Ñ Ð±Ð¾Ð»ÑÐ½Ð¸ÑÐ°" Ð¿ÑÐ¸ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐµ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ñ Ñ Ð¿Ð°ÑÐ¸ÐµÐ½ÑÐ°. Ð Ð°Ð±Ð¾ÑÐ° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ Ð¤ÐÐ£Ð ÐÐÐÐ­Ð Ð¸Ð¼. ÐÐ°ÑÑÐµÑÐ°. ÐÑÐ½Ð¾Ð²Ð½ÑÐ¼Ð¸ Ð¼ÐµÑÐ¾Ð´Ð°Ð¼Ð¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐ²Ð»ÑÐ»Ð¸ÑÑ: Â«ÐÑÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ðµ Ð±Ð¸Ð¾Ð¿Ð»ÐµÐ½Ð¾Ðº Ð½Ð° 96-Ð»ÑÐ½Ð½Ð¾Ð¼ Ð¿Ð»Ð°Ð½ÑÐµÑÐµ Ð² ÐÐÐÂ», Â«ÐÐ°ÑÐ°Ð»Ð»ÐµÐ»ÑÐ½Ð¾Ðµ Ð²ÑÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ðµ Ð±Ð¸Ð¾Ð¿Ð»ÐµÐ½Ð¾Ðº Ð½Ð° 96-Ð»ÑÐ½Ð½Ð¾Ð¼ Ð¿Ð»Ð°Ð½ÑÐµÑÐµ Ð² ÐÐÐ Ñ Ð´Ð¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸Ñ 0,5 % ÑÐ°ÑÑÐ²Ð¾ÑÐ° Ð³Ð»ÑÐºÐ¾Ð·ÑÂ» Ð¸ Â«ÐÐ°ÑÐ°Ð»Ð»ÐµÐ»ÑÐ½Ð¾Ðµ Ð²ÑÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ðµ Ð±Ð¸Ð¾Ð¿Ð»ÐµÐ½Ð¾Ðº Ð½Ð° 96-Ð»ÑÐ½Ð½Ð¾Ð¼ Ð¿Ð»Ð°Ð½ÑÐµÑÐµ Ð² ÐÐÐ Ð¸ Ð² ÑÐ¸Ð·ÑÐ°ÑÑÐ²Ð¾ÑÐµÂ». ÐÐ¾ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ°Ð¼ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ° Ð±ÑÐ»Ð¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ñ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð² Ð±Ð¸Ð¾Ð¼Ð°ÑÑÐµ Ð¿ÑÐ¸ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ ÑÑÐ»Ð¾Ð²Ð¸ÑÑ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÑÑ. P. Ð°eruginosa 7681.Â 
description: The objectives of the study: to explore the growth and development of biofilm structures formed by the microorganism P. aeruginosa under various cultivation conditions, the transformation of the obtained experimental data to calculate the biomass of the obtained biofilms. The object of the study is the strain P. aeruginosa 7681, obtained from the biological material of the patient of the St. Petersburg State Medical Institution "Elizabethan Hospital" during the diagnosis of the disease in the patient. The work was carried out on the basis of the Saint-Petersburg Pasteur Institute. The main research methods were: "Cultivation of biofilms on a 96-moon tablet in beef-extract broth", "Parallel cultivation of biofilms on a 96-moon tablet in beef-extract broth with the addition of 0,5 % glucose solution" and "Parallel cultivation of biofilms on a 96-moon tablet in beef-extract broth and in saline". According to the results of the study, changes in biomass were investigated under various conditions of cultivation of strain P. aeruginosa 7681.Â