title: Ð Ð°Ð·Ð»Ð¸ÑÐ½ÑÐµ ÑÐ¿Ð¾ÑÐ¾Ð±Ñ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÐºÐ°Ð¿ÑÐ¾Ð»Ð°ÐºÑÐ¾Ð½Ð¾Ð²ÑÑ Ð¼Ð°ÑÑÐ¸Ñ, Ð¿ÑÐµÐ´Ð½Ð°Ð·Ð½Ð°ÑÐµÐ½Ð½ÑÑ Ð´Ð»Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÐºÐ»ÐµÑÐ¾Ðº
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Various methods of modification of polycaprolactone matrices intended for cell culture
Creators: name: Ð§Ð°Ð±Ð¸Ð½Ð°, ÐÐ»Ð¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: ÐÐ¾Ð¼Ð¿Ð¾Ð·Ð¸ÑÐ¸Ð¾Ð½Ð½ÑÐµ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ñ
ÐÐ»ÐµÑÐºÐ° (Ð±Ð¸Ð¾Ð».)
ÐÐ¾Ð»Ð¸Ð¼ÐµÑÑ
Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ñ
Ð°Ð¼Ð¸Ð½Ð¾Ð³ÑÑÐ¿Ð¿Ð°
modification
amino group

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Ð¿Ð¾Ð»Ð¸ÑÑÐ¸ÑÐ¾Ð², Ð² ÑÐ°ÑÑÐ½Ð¾ÑÑÐ¸ Ð¿Ð¾Ð»Ð¸-É-ÐºÐ°Ð¿ÑÐ¾Ð»Ð°ÐºÑÐ¾Ð½Ð° (ÐÐÐ), Ð²Ð²ÐµÐ´ÐµÐ½Ð¸ÐµÐ¼ ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð°Ð»ÑÐ½ÑÑ Ð³ÑÑÐ¿Ð¿, Ð° Ð¸Ð¼ÐµÐ½Ð½Ð¾ Ð°Ð¼Ð¸Ð½Ð¾Ð³ÑÑÐ¿Ð¿ Ð¸ Ð³Ð¸Ð´ÑÐ¾ÐºÑÐ¸Ð»ÑÐ½Ð¾Ð¹ Ð³ÑÑÐ¿Ð¿Ñ, Ð½Ð° Ð¿Ð¾Ð²ÐµÑÑÐ½Ð¾ÑÑÑ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ¾Ð². ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: 1. ÐÐ¾Ð»ÑÑÐ¸ÑÑ Ð¼Ð°ÑÑÐ¸ÑÑ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ ÐÐÐ Ñ ÑÐ°Ð·Ð½Ð¾Ð¹ Ð¿Ð¾Ð²ÐµÑÑÐ½Ð¾ÑÑÐ½Ð¾Ð¹ ÐºÑÐ¸ÑÑÐ°Ð»Ð»Ð¸ÑÐµÑÐºÐ¾Ð¹ ÑÑÑÑÐºÑÑÑÐ¾Ð¹ Ð¸ Ð¸Ð·ÑÑÐ¸ÑÑ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð²Ð²ÐµÐ´ÐµÐ½Ð¸Ñ Ð°Ð¼Ð¸Ð½Ð¾Ð³ÑÑÐ¿Ð¿Ñ Ð·Ð° ÑÑÐµÑ Ð°ÑÐ³Ð¸Ð½Ð¸Ð½Ð¾Ð²Ð¾Ð¹ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Ð½Ð° ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° Ð¿Ð¾Ð²ÐµÑÑÐ½Ð¾ÑÑÐ¸ ÐÐÐ Ð¼Ð°ÑÑÐ¸Ñ; 2. ÐÐ·ÑÑÐ¸ÑÑ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð²Ð²ÐµÐ´ÐµÐ½Ð¸Ñ Ð°Ð¼Ð¸Ð½Ð¾Ð³ÑÑÐ¿Ð¿Ñ Ð¸ Ð³Ð¸Ð´ÑÐ¾ÐºÑÐ¸Ð»ÑÐ½Ð¾Ð¹ Ð³ÑÑÐ¿Ð¿Ñ Ð½Ð° Ð±Ð¸Ð¾ÑÐ¾Ð²Ð¼ÐµÑÑÐ¸Ð¼Ð¾ÑÑÑ Ð¿Ð¾Ð»Ð¸ÑÑÐ¸ÑÐ¾Ð² Ð·Ð° ÑÑÐµÑ ÑÐ¾Ð·Ð´Ð°Ð½Ð¸Ñ ÐºÐ¾Ð¼Ð¿Ð¾Ð·Ð¸ÑÐ½ÑÑ Ð¼Ð°ÑÑÐ¸Ñ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ ÑÐ¼ÐµÑÐ¸ Ð¿Ð¾Ð»Ð¸ÑÑÐ¸ÑÐ¾Ð² Ð¸ ÐÐ­Ð/ÐÐ­Ð-2NH2; 3. ÐÑÐµÐ½Ð¸ÑÑ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¹ Ð½Ð° Ð°Ð´Ð³ÐµÐ·Ð¸Ñ Ð¸ Ð¿ÑÐ¾Ð»Ð¸ÑÐµÑÐ°ÑÐ¸Ñ ÐÐ¡Ð. Ð Ð°Ð±Ð¾ÑÐ° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ ÐÐ½ÑÑÐ¸ÑÑÑÐ° ÑÐ¸ÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð ÐÐ, Ð³Ð´Ðµ Ð±ÑÐ»Ð° ÑÐ´ÐµÐ»Ð°Ð½Ð° Ð±Ð¾Ð»ÑÑÐ°Ñ ÑÐ°ÑÑÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹: Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ðµ Ð¾Ð±ÑÐ°Ð·ÑÐ¾Ð² Ð¸ Ð¸Ñ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ñ, Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ, Ð¿Ð¾Ð´ÑÑÐµÑ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð° ÐºÐ»ÐµÑÐ¾Ðº Ð¸ Ð¸Ð·Ð¼ÐµÑÐµÐ½Ð¸Ðµ ÑÐ³Ð»Ð¾Ð² ÑÐ¼Ð°ÑÐ¸Ð²Ð°Ð½Ð¸Ñ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ¾Ð². ÐÑÐ» Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½ Ð°Ð½Ð°Ð»Ð¸Ð· Ð²Ð·Ð°Ð¸Ð¼Ð¾ÑÐ²ÑÐ·Ð¸ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð², Ð¿Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÑÑÐ¸Ñ, ÐºÐ°ÐºÐ¸Ð¼ Ð¾Ð±ÑÐ°Ð·Ð¾Ð¼ ÑÑÑÑÐºÑÑÑÐ° Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ° Ð¸ Ð²Ð¸Ð´ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÑÑ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð½Ð° ÑÐºÐ°Ð½ÐµÐ¸Ð½Ð¶ÐµÐ½ÐµÑÐ½ÑÑ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ñ. ÐÐ½Ð°Ð»Ð¸Ð· Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»Ð¸ Ð² Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ð°Ñ ImageJ, MS Office, Adobe Photoshop, GraphPad Prism 9. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð±ÑÐ»Ð¸ Ð¿ÑÐ¾Ð°Ð½Ð°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°Ð½Ñ Ð²Ð°ÑÐ¸Ð°Ð½ÑÑ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ ÐÐÐ Ð¼Ð°ÑÑÐ¸Ñ. ÐÐ°Ð»Ð¾Ð¶ÐµÐ½Ð° Ð¾ÑÐ½Ð¾Ð²Ð° Ð¿ÑÐµÐ¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ñ ÐÐÐ Ð¼Ð°ÑÑÐ¸ÑÑ Ð² Â«ÑÐ¼Ð°ÑÑ-Ð¿Ð¾Ð²ÐµÑÑÐ½Ð¾ÑÑÑÂ». ÐÐ° Ð¾ÑÐ½Ð¾Ð²Ð°Ð½Ð¸Ð¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð½ÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ Ð² ÐÐÐ¦ Ð ÐÐ Ð±ÑÐ»Ð¸ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ñ Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð¸Ñ Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐµÐ³Ð¾ Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð¼Ð°ÑÑÐ¸Ñ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ ÐÐÐ Ñ Ð°ÑÐ³Ð¸Ð½Ð¸Ð½Ð¾Ð²Ð¾Ð¹ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸ÐµÐ¹.
description: This work is devoted to the studying of modification of polyesters, such as poly-É-caprolactone (PCL), by the introduction of functional groups, such as amino groups and hydroxyl groups, on the surface of polymers. The research set the following goals: 1. To obtain PCL matrices with different surface crystal structures and to study the effect of the introduction of an amino group due to arginine modification on the surface properties of PCL matrices; 2. To study the effect of the introduction of amino groups and hydroxyl groups on the biocompatibility of polyesters by creating composite matrices based on a mixture of polyesters and PEG/PEG-2NH2; 3. Evaluate the effect of modifications on the adhesion and proliferation of MSCs. The work was fulfilled on the premises of the Institute of Cytology of the Russian Academy of Sciences, where most of the experimental studies were car-ried out: matrices obtaining and modification, microscopy, counting the number of cells and studying wetting angles of polymers. The analysis of the relationship between factors shows us how the structure of the polymer and the type of modifica-tion affect the tissue engineering construction. The analysis was carried out in such programs, as ImageJ, MS Office, Adobe Photoshop, GraphPad Prism 9. As a result, different variants of PCL matrices modifications were analyzed. The basis for the transformation of the PCL matrix into a "smart surface" was laid. Based on the research conducted at the INC RAS, the horizons for further studies of such matrices with arginine modifications were stated.