cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Development of a toroidal-shaped differential mobility analyzer for effective measurements of airborne particles: Experiment and modeling
Creators: name: Lee, Handol
name: Lee, Gun Ho
name: Ahn, Kang-Ho
Publisher: name: Taylor & Francis
Year: 2019
Subjects: subject: Cell Biology
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: FOS: Biological sciences
subject: Cancer

description: A toroidal-shaped differential mobility analyzer (DMA), called toroidal Hanyang-DMA (toroidal Hy-DMA), was developed for particle characterization. The height, width, and weight of the newly developed toroidal Hy-DMA are 8 cm, 14 cm, and 1.2 kg, respectively, indicating that it is much more compact and lighter than the TSI long-DMA; nevertheless, the classifiable particle size range is up to 400 nm. Therefore, the toroidal Hy-DMA can be useful for many applications in a limited space owing to its small size. The performance of the Hy-DMA was evaluated using tandem differential mobility analyzer (TDMA) experiments with two identical Hy-DMAs and numerical simulations using a single-particle tracking analysis in a Lagrangian framework. To simulate particle behaviors, a flow-rate-weighted particle injection method, which is more realistic, was employed, and the proposed particle tracking method can be widely applied to any non-plug flow conditions. The obtained experimental data and numerical results of central particle sizes are consistent with each other. Empirical and numerical transfer functions of the toroidal Hy-DMA were obtained and compared with those of other types of well-known DMAs. It is concluded that the toroidal Hy-DMA has an empirical transmission probability ranging from 0.5 to 0.9 and a sizing resolution ranging from 5.5 to 9.0, which indicates acceptable performance in classifying monodisperse particles within the test size range of 20 to 400 nm. Copyright © 2019 American Association for Aerosol Research
beta-globin transcript found in induced murine erythroleukemia cells is homologous to the beta h0 and beta h1 genes.
Creators: name: Brown, B. A.
name: Hardies, S. C.
name: Edgell, M. H.
name: Padgett, R. W.
name: Hutchison, C. A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1982

description: RNA transcripts complementary to at least one of the four beta-globin homologous genes (beta h) are found in high concentration in the murine erythroleukemic (MEL) cell line GM979 after butyric acid induction. Hybridization data indicate that the gene expressed is Hbb-beta h0 or Hbb-beta h1, or both. The level of beta h0/1 transcripts in the MEL cell is similar to the level of adult transcripts. The Hbb-beta h0/1 transcript is about 800 nucleotides in length. In addition, there is a larger beta h0/1 transcript of the same size and relative intensity as the adult beta-globin precursor. We also report significant levels of embryonic gene Hbb-y transcripts in induced GM979 cells. We have determined that the GM979 cell line has the [Hbb]s haplotype on the basis of an examination of its globin DNA restriction pattern. An additional MEL cell line of haplotype [Hbb]d (DBA/2 line 6A11A) was examined and found to contain no significant level of Hbb-beta h0, Hbb-beta h1, Hbb-beta h2, or Hbb-y gene transcripts.
In situ aerosol acidity measurements using a UV–Visible micro-spectrometer and its application to the ambient air
Creators: name: Jang, Myoseon
name: Sun, Shiqi
name: Winslow, Ryan
name: Han, Sanghee
name: Yu, Zechen
Publisher: name: Taylor & Francis
Year: 2020
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Genetics
subject: Pharmacology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: 20199 Astronomical and Space Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: 69999 Biological Sciences not elsewhere classified

description: An in situ analytical method was demonstrated to measure the proton concentration ([H+]C-RUV) of an aerosol particle by using colorimetry integrated with a Reflectance UV-Visible spectrometer (C-RUV). Acidic particles comprising ammonium, sulfate, and water were generated in a flow tube under varying humidity and employed to calibrate the method using the inorganic thermodynamic models (i.e., E-AIM and ISORROPIA). The predictive [H+]C-RUV equation derived using strongly acidic compositions was then extended to ammonia-rich aerosols, which were lacking in the database of the thermodynamic models. The predictive [H+]C-RUV equation was also expanded to aerosols composed of sodium, ammonium, and sulfate. [H+]C-RUV generally agrees with both E-AIM predicted [H+] and ISORROPIA predicted [H+] for highly acidic aerosols, or aerosols at high humidity. For ammonia-rich aerosols under low humidity, [H+]C-RUV disagrees with that predicted from inorganic thermodynamic models. C-RUV was feasible for ambient aerosols because colorimetry is specific to aerosol acidity. Most aerosols collected at the University of Florida between 2018 and 2019 were acidic. Sodium ions appeared during the spring and summer, as coastal sea breezes traveled inland. The concentrations of ammonium and nitrate were high in the winter due to the phase partitioning of nitric acid and ammonia gases. The fraction of non-electrolytic dialkyl-organosulfate (diOS) to total sulfate is estimated by comparing the actual particle [H+] measured by C-RUV to the [H+] predicted using the inorganic composition and the inorganic thermodynamic models. The diOS fraction varied from 0% to 60% and was higher in the summer months when [H+] is high. Copyright © 2020 American Association for Aerosol Research
Circulant orbitals and the electron-correlation problem
Creators: name: Chen, M.-B.
name: Parr, R. G.
name: Pauncz, R.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1982

description: Circulant, as well as canonical, orbitals are used in the different orbitals for different spins method for treating electron correlation. Circulant orbitals provide a theoretical justification for the use of a single parameter, even when the canonical orbitals have widely different orbital energies. Illustrative calculations on the ground state of the Be atom show the importance of choosing the correct "pairs" in the method. A two-parameter version of the conventional method gives 74% of the improvement obtained by a full configuration-interaction treatment using 20 linear parameters, while a one-parameter linear combination of two different coupling schemes of the circulant method gives approximately 61%. The latter wavefunction provides a compact description of the electron correlation.
Collagen structural microheterogeneity and a possible role for glycosylated hydroxylysine in type I collagen
Creators: name: Yamauchi, M.
name: Mechanic, G. L.
name: Noyes, C.
name: Kuboki, Y.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1982

description: A three-chained peptide from type I collagen, crosslinked by hydroxyaldolhistidine, has been isolated from a tryptic digest of 5 M guanidine.HCl-insoluble bovine skin collagen (a small but as yet unknown percentage of the total collagen in whole skin). OsO(4)/NaIO(4) specifically cleaved the crosslink at its double bond into a two-chained crosslink peptide and a single peptide. The sequence of the two-chained peptide containing the bifunctional crosslink was determined after amino acid analysis of the separated peptides. The crosslink consists of an aldehyde derived from hydroxylysine-87 in the aldehyde-containing cyanogen bromide fragment alpha1CB5(ald) and an aldehyde derived from the lysine in the COOH-terminal nonhelical region of the alpha1CB6(ald) fragment. The alpha1CB6(ald) portion of the peptide exhibited structural microheterogeneity, containing the inverted sequence Ala-Lys-His instead of the normal sequence Lys-Ala-His. This indicates that another structural gene exists for alpha1(I) chain. The original three-chained peptide did not contain any glycosylated hydroxylysine or glycosylated hydroxyaldolhistidine. The lack of glycosylation of hydroxylysine-87 in alpha1CB5, which is usually glycosylated, allowed formation of the aldehyde, and this, coupled with the sequence inversion, may have allowed formation of the nonreducible crosslink hydroxyaldolhistidine. We suggest that the role of glycosylation, a posttranslational modification, of specific hydroxylysine residues is to prevent their oxidative deamination to aldehydes, thereby precluding formation of complex stable crosslinks. Complex crosslinks would decrease the rate of collagen turnover. The decrease, with time, would increase the population of stable crosslinked collagen molecules, which would eventually accumulate with age.
Cycloartenol-derived sterol biosynthesis in the Peronosporales
Creators: name: Sovocool, G. W.
name: Domnas, A. J.
name: Eierman, D. F.
name: Warner, S. A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1982

description: Selected species of the order Peronosporales, which are unable to epoxidize squalene and thus synthesize sterols, are able to metabolize exogenous cycloartenol to lanosterol and in some organisms to fucosterol, ergosterol, and cholesterol. Lanosterol was less effectively utilized but some ergosterol and cholesterol were yielded. Fucosterol was very efficiently metabolized by most species to ergosterol, Delta(7)-ergostenol, Delta(5)-ergostenol, cholestanol, and cholesterol. Several unknown sterols were observed in most trials. These data suggest a vestigial sterol synthetic pathway derived from cycloartenol, followed by possible isomerization to lanosterol and then to other sterols.
DNA precursor pool: a significant target for N-methyl-N-nitrosourea in C3H/10T1/2 clone 8 cells.
Creators: name: Baker, M. S.
name: Topal, M. D.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1982

description: Synchronized C3H/10T1/2 clone 8 cells were treated in vitro with a nontoxic dose of N-methyl-N-nitrosourea during their S phase. Chromatographic isolation of the deoxyribonucleotide DNA precursor pool and measurement of the precursor content per cell showed that a nucleic acid residue in the precursor pool is 190-13,000 times more susceptible to methylation than a residue in the DNA duplex, depending on the site of methylation. This conclusion comes from measurements indicating that, for example, the N-1 position of adenine in dATP is 6.3 times more methylated than the same position in the DNA, even though the adenine content of the pool is only a fraction (0.0005) of the adenine content of the DNA helix. The comparative susceptibility between pool and DNA was found to vary with the site of methylation in the order the N-1 position of adenine greater than phosphate greater than the N-3 position of adenine greater than the O6 position of guanine greater than the N-7 position of guanine. The significance of these results for chemical mutagenesis and carcinogenesis is discussed.
title: Advancing Research Through DataCite’s Global Access Fund: National Science Library of Georgia
Creators: name: Pavliashvili, Nino
name: Giorgobiani, Natali
name: Mostafa, Mohamad
Publisher: name: DataCite
publisherIdentifier: https://ror.org/04wxnsj81
publisherIdentifierScheme: ROR
Year: 2024

description: The DataCite Global Access Fund (GAF) is a strategic initiative designed to enhance equitable access to research infrastructure by supporting organizations in regions and communities currently underrepresented in the global open science infrastructure landscape. In this blog post series, we will explore the projects of the GAF awardees, sharing insights into the outcomes and future aspirations. Each of the blog posts will focus on how the projects lead to the creation of a more inclusive global research community, sharing what they have achieved so far and outlining their plans for further development and impact.

Supported by funding from the DataCite Global Access Fund (GAF), the National Science Library of Georgia is undertaking a significant project to develop a research infrastructure – Openscience.ge – an open-access DSpace-based digital repository of Georgian scientific works. The project aims to enhance the collection and dissemination of scientific materials, including journal articles, conference materials, theses, and dissertations, etc, and to create comprehensive researcher profiles. The primary goal of the project is to establish and advance a national repository that will consolidate all scientific outputs produced by Georgian scientists and researchers. The project was launched in January 2024 and will be finished by December of this year.
MPEC 2023-F234 : 2023 FS11
Creators: name: Minor Planet Center Staff
Publisher: name: The Minor Planet Center
Year: 2023
Subjects: subject: Small Solar System bodies
valueUri: http://astrothesaurus.org/uat/1469
subject: 2023 FS11

description: The Minor Planet Electronic Circulars contain information on unusual minor planets, routine data on comets and natural satellites, and occasional editorial announcements.  They are published on behalf of Division F of the International Astronomical Union by the Minor Planet Center, Smithsonian Astrophysical Observatory, Cambridge, MA 02138, U.S.A.
Spectral Time Series Analysis of Ocean Wave Buoy Measurements
Creators: name: Grainger, Jake
Publisher: name: Lancaster University
Year: 2022

description: Waves in the ocean can be as dangerous as they are impressive. In order to study the behaviour of such waves, buoys are commonly deployed to collect recordings of the ocean surface over time. This results in large quantities of high-frequency multivariate time series data. The statistical analysis of such data is of great importance in a variety of engineering and scientific contexts, from the design of coastal flood defences to offshore structures. We develop methodology for analysing such buoy data, investigating two key questions. Firstly, how should we perform parameter inference for models of the frequency domain behaviour of the surface, given recorded buoy data? Secondly, how can we detect statistically significant non-linearities present in these time series? For parameter inference, we find that pseudo-likelihood approaches greatly outperform state-of-the-art methodologies. As a result, not only can we obtain more reliable parameter estimates, but we can also perform inference for more complicated models, allowing for a more intricate description of the waves. Due to the improved performance of such estimates, we are able to see the evolution of these parameters throughout storm events, using recorded buoy data from both California and the North Sea. For detecting non-linearities, we develop a robust testing procedure by evaluating the bispectrum of the observed time series against the bispectrum of bootstrap simulated Gaussian processes with similar characteristics. We explore the performance of this technique in simulation studies, and apply the approach to buoy data from California.
Supplementary document for Miniaturized fiber optic accelerometer incorporating liquid metal as proof mass - 7329075.pdf
Creators: name: Xue, Chenxing
name: Zhou, Rui
name: Xia, Yuanjia
name: Gao, Wei
name: Li, Min
name: Gao, Hong-Yun
name: Wen, Xiaoyan
name: Lu, Haifei
name: Chen, Guobing
name: chen, desheng
Publisher: name: Optica Publishing Group
Year: 2025
Subjects: Uncategorized

description: Supplemental Document
Precision characterization of three ultrafine condensation particle counters using singly charged salt clusters in the 1–4 nm size range generated by a bipolar electrospray source
Creators: name: Brilke, Sophia
name: Resch, Julian
name: Leiminger, Markus
name: Steiner, Gerhard
name: Tauber, Christian
name: Wlasits, Peter J.
name: Winkler, Paul M.
Publisher: name: Taylor & Francis
Year: 2020
Subjects: subject: Biophysics
subject: 29999 Physical Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: Medicine
subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences

description: Molecular singly charged clusters generated by electrospray sources are commonly used for calibration measurements of ultrafine Condensation Particle Counters (CPCs) in the sub-2 nm size range. This technique has been limited to the smallest singly charged clusters. In this study, we used a bipolar electrospray source combining two electrosprays of opposite polarities to generate singly charged clusters of ammonium salts dissolved in acetonitrile, i.e., tetra-heptyl ammonium bromide (THABr) and tetra-butyl ammonium iodide (TBAI) clusters. A high-resolution UDMA acts as a classifier for positively and negatively charged clusters which are used as mobility standards for the measurement of the complete ascent of the detection efficiency curve of three ultrafine CPCs. The CPCs characterized in this work are two laminar flow-type CPCs using n-butanol (TSI UCPC Model 3776, TSI Inc., Minneapolis, MN USA) and diethylene glycol (DEG) (TSI UCPC Model 3777, TSI Inc.) as working fluid and the turbulent mixing-type DEG-based Particle Size Magnifier (Airmodus A10 PSM). The clusters generated by the bipolar electrospray source are analyzed for their elemental composition using the Atmospheric Pressure interface Time-Of-Flight mass spectrometer (ioniAPi-TOF, Ionicon Analytik GmbH, Austria) in positive and negative ion mode. We present mobility-dependent mass spectra from experiments using the UDMA and the ioniAPi-TOF in series for the analysis of positively charged clusters when operating the electrospray source in unipolar and in bipolar mode to demonstrate the charge reduction and purity of the sample.
Escherichia coli single-strand binding protein organizes single-stranded DNA in nucleosome-like units.
Creators: name: Griffith, J.
name: Chrysogelos, S.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1982

description: Electron microscopy shows that complexes of the single-strand DNA binding protein (SSB) of Escherichia coli and phage fd DNA appear as beaded fiber loops containing an average of 38 beads, 1 per 170 bases of DNA. Extensive digestion of native unfixed SSB-fd DNA complexes with micrococcal nuclease reveals a protected DNA fragment of 145 bases, while shorter digestion periods result in a sequence of fragments in multiples of 160 +/- 25 bases. Digestion of these complexes with DNase I produces a repeating pattern of bands, multiples of approximately 15 bases with strong bands at 60, 105, 118, 130, 145, 150, and 210 bases. Isopycnic banding in CsCl solution yields densities of 1.272 and 1.700 g/ml, respectively, for SSB alone and for fd DNA and, after fixation, of 1.388 g/ml for fd DNA-SSB beaded fibers and 1.373 g/ml for the individual protein-DNA beads. Based on these data and the molecular weights of SSB and fd DNA, we suggest that the nucleoprotein chain consists of eight molecules of SSB bound to 145 bases of DNA, with these units linked by roughly 30 bases of protein-free DNA. The excellent concord between results obtained by enzyme digestion of unfixed native samples and, after fixation, by electron microscopy and density banding supports the conclusion that SSB organizes single-stranded DNA in a manner similar to the organization of duplex DNA by histones.
Repositórios e Plano de Gestão de Dados: o caso IPAlliance
Creators: name: Lopes, Mafalda
name: Padilha, Miguel
name: Vieira, Francisco
name: Karimova, Yulia
Publisher: name: Zenodo
Year: 2023
Subjects: Data management plan
Open Science
IPAlliance

description: Demonstrar que a criação de um Plano de Gestão de Dados (PGD) contribui para uma melhor organização do projeto, dando suporte aos investigadores na tomada de decisão em questões relacionadas com a Gestão de Dados de Investigação (GDI) e permitindo tomar medidas de proteção e uso de dados pessoais.
Hormonal regulation of protein synthesis, secretion, and phosphorylation in cultured rat Sertoli cells.
Creators: name: Kierszenbaum, A. L.
name: DePhilip, R. M.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1982

description: The accumulation of two polypeptides, SCm1 and SCm2, in the medium of Sertoli cell cultures is enhanced by follicle-stimulating hormone (FSH) but is unaffected by either the cAMP analog, N6,O2'-dibutyrl cAMP or luteinizing hormone. The assigned molecular weights of SCm1 and SCm2 differ from those of androgen-binding protein subunits or any other previously identified Sertoli cell secretory product. Incubation of Sertoli cell cultures with either FSH or N6,O2'-dibutyryl cAMP also stimulates the incorporation of [35S]methionine into two intracellular polypeptides, SCc1 and SCc2. In addition, the phosphorylation of three intracellular polypeptides, SCc3, SCc4, and SCc5, is intensified when Sertoli cell cultures are treated with either FSH or N6,O2'-dibutyryl cAMP. Based on these results and on previous work, we conclude that (i) SCm1 and SCm2 may, like androgen-binding protein, be secreted by Sertoli cells and function extracellularly while SCc1 and SCc2 are involved in FSH-dependent intracellular activity; (ii) SCc3, SCc4, and SCc5 are possible substrates for FSH-stimulated, cAMP-dependent protein kinase activity; and (iii) SCc5 is an isoelectric variant of vimentin-type intermediate filament protein presumably involved in FSH- and N6,O2'-dibutyryl cAMP-induced Sertoli cell shape changes.
MPEC 2023-F235 : 2023 FT11
Creators: name: Minor Planet Center Staff
Publisher: name: The Minor Planet Center
Year: 2023
Subjects: subject: Small Solar System bodies
valueUri: http://astrothesaurus.org/uat/1469
subject: 2023 FT11

description: The Minor Planet Electronic Circulars contain information on unusual minor planets, routine data on comets and natural satellites, and occasional editorial announcements.  They are published on behalf of Division F of the International Astronomical Union by the Minor Planet Center, Smithsonian Astrophysical Observatory, Cambridge, MA 02138, U.S.A.
Pollen Clustering Strategies Using a Newly Developed Single-Particle Fluorescence Spectrometer
Creators: name: Swanson, Benjamin E.
name: Huffman, J. Alex
Publisher: name: Taylor & Francis
Year: 2020
Subjects: subject: Biophysics
subject: Space Science
subject: Molecular Biology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Ecology
subject: FOS: Biological sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences

description: Pollen is routinely monitored and forecasted with respect to public health and allergies, but monitoring networks generally utilize a manual process of collection, analysis, and modeling that leads to poor sampling density and high measurement cost. Here, we discuss application of a single-particle fluorescence sensor recently developed for the purpose of real-time detection and recognition of pollen and spores. The sensor operates by collecting fluorescence emission spectra from many individual pollen grains sampled onto a microscope slide for each of four excitation wavelengths (280, 350, 405, and 450 nm) associated with pollen fluorophores. The sensor also records major and minor diameters of each particle. Approximately 25-30 particles for each of eight commercially purchased pollen species were interrogated. Data were analyzed using four classification methods: hierarchical agglomerative and k-means clustering (unsupervised) and random forest and gradient boosting algorithms (supervised). The purpose of the manuscript is to show development of a computational strategy to analyze spectral input data of this kind in order to support further efforts to automate sensor data collection and analysis. Both unsupervised methods showed insufficient accuracy for separating pollen species (76% k-means, 9% HAC) whereas supervised methods performed similarly well (94-95%). The random forest algorithm was then utilized to further optimize operational parameters, based on its higher computational speed. Analyzing the relative importance of each optical source for sensor performance highlighted ways that may be useful to lower sensor cost with minimal reduction to analysis quality. The results provide a framework for the application of this and similar sensors to ambient pollen detection and classification.
Hydrolysis of human platelet membrane glycoproteins with a Serratia marcescens metalloprotease: Effect on response to thrombin and von Willebrand factor
Creators: name: Wagner, R. H.
name: Cooper, H. A.
name: White, G. C.
name: Bennett, W. P.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1982

description: Metalloproteases from at least two Gram-negative organisms selectively hydrolyze the surface of human fixed washed platelets. The protease-treated platelets lose their ability to aggregate with bovine von Willebrand factor and human von Willebrand factor plus ristocetin. The present study reports the membrane glycoprotein alterations and loss of platelet function that occur after incubation of fresh washed human platelets with a purified protease produced by
Interpretation of Volatility Tandem Differential Mobility Analyzer (V-TDMA) Data for Accurate Vapor Pressure and Enthalpy Measurement: Operational Considerations, Multiple Charging, and Introduction to a New Analysis Program (TAO)
Creators: name: Oxford, Christopher R.
name: Dang, Audrey J.
name: Rapp, Charles M.
name: Williams, Brent J.
Publisher: name: Taylor & Francis
Year: 2020
Subjects: subject: Space Science
subject: 29999 Physical Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences

description: Significant evaporation of pure aerosols in a Volatility Tandem Differential Mobility Analyzer (V-TDMA) creates two Condensation Particle Counter (CPC) response peaks. Two hypotheses for the observed peaks have been proposed: the existence of two phases or the separation of the singly charged experimental size distribution from the remaining experimental size distributions with charges greater than 1 (charge separation). To explore this observation, we atomized pure levoglucosan aerosol and evaporated the aerosol until two peaks formed. We used an additional classifier and neutralizer to select particles from each of the two peaks and assessed the number of charges on the particles. The smaller diameter peak contained singly charged particles, and the larger diameter peak contained the remaining charges. The charge separation hypothesis alone accounts for the two-peak observations. We used a new V-TDMA model named TAO and show that charge separation should occur in other pure components as well. The TAO model was then used to display the impact of different DMA transfer functions, different inlet size distributions, and different oven residence time distributions (RTDs) on the CPC response. Large errors are possible when direct measurement of the RTD is not performed or when wide RTDs are used. We recommend use of narrow transfer functions with narrow RTDs to detect charge separation. When the singly charged CPC response is isolated (smaller diameter peak in the two peak response), accurate estimations of vapor pressure can be recovered, assuming accurate values for gas phase diffusivity, surface energy, particle density, etc. are used.
Pre trained models used in the paper "A Note on the Regularity of Images Generated by Convolutional Neural Networks"
Creators: name: Habring, Andreas
Publisher: name: Zenodo
Year: 2023

description: Pre-trained network weights to reproduce the results shown in the paper "A Note on the Regularity of Images Generated by Convolutional Neural Networks" by Andreas Habring and Martin Holler and to appear in SIAM Journal on Mathematics of Data Science.
description: {"references": ["Habring, Andreas, and Martin Holler. \"A Note on the Regularity of Images Generated by Convolutional Neural Networks.\" (2023)"]}
Modulation of the epidermal growth factor receptor by platelet-derived growth factor and choleragen: Effects on mitogenesis
Creators: name: Pledger, W. J.
name: Wharton, W.
name: O'Keefe, E. J.
name: Leof, E.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1982

description: The addition of fresh medium supplemented with partially purified platelet-derived growth factor (PDGF) to quiescent density-arrested cultures of BALB/c-3T3 cells decreases the subsequent binding of radiolabeled epidermal growth factor (EGF). The decrease in EGF binding can be observed 1 hr after the addition of PDGF. This effect is maximal in 2-3 hr, and binding remains diminished for at least 6 hr. These effects can be accounted for by a decrease in the number of EGF receptors with no change in receptor affinity. The action of PDGF is concentration dependent, but even at very high concentrations of PDGF the reduction in EGF binding is never more than 50%. Similar decreases in EGF binding are produced by other treatments that render BALB/c-3T3 cells competent, such as the addition of fibroblast growth factor or medium previously exposed to the macrophage-like cell line P388D(1). Cholera toxin (choleragen), which alone had no effect on EGF binding, dramatically potentiated the ability of PDGF to down regulate EGF receptors. Two to three hours after the addition of PDGF and choleragen, EGF binding was reduced by 80-90% compared with control values. The ability of PDGF and choleragen together to decrease EGF binding was substantially inhibited by cycloheximide. Autoradiography of [(3)H]thymidine-labeled cells shows that choleragen potentiates the action of PDGF; lower concentrations of PDGF are required to make cells competent after choleragen treatment. Furthermore, cells treated with PDGF and choleragen no longer require EGF for traverse of G(1) phase and initiation of DNA synthesis in defined medium. The reduction in receptor number produced by choleragen and PDGF, which may be due to internalization of the EGF receptor, may mimic the action of EGF and thereby remove the EGF requirement for DNA synthesis.
Repositórios e Plano de Gestão de Dados: o caso IPAlliance
Creators: name: Lopes, Mafalda
name: Padilha, Miguel
name: Vieira
name: Karimova, Yulia
Publisher: name: Zenodo
Year: 2023
Subjects: Data management plan
Open Science
IPAlliance

description: Demonstrar que a criação de um Plano de Gestão de Dados (PGD) contribui para uma melhor organização do projeto, dando suporte aos investigadores na tomada de decisão em questões relacionadas com a Gestão de Dados de Investigação (GDI) e permitindo tomar medidas de proteção e uso de dados pessoais.
Periportal and pericentral pyridine nucleotide fluorescence from the surface of the perfused liver: evaluation of the hypothesis that chronic treatment with ethanol produces pericentral hypoxia.
Creators: name: Lemasters, J. J.
name: Ji, S.
name: Christenson, V.
name: Thurman, R. G.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1982

description: Pyridine nucleotide fluorescence made from the surface of the hemoglobin-free perfused rat liver was measured continuously by using a "micro-light guide" placed on selected periportal and pericentral regions of the liver lobule. From the portal oxygen tension at which pyridine nucleotide reduction first occurred in pericentral regions, the oxygen gradient across the liver lobule was estimated in livers from rats treated chronically with ethanol or sucrose. Chronic treatment with ethanol increased the average lobular oxygen gradient from 275 to 400 torr (1 torr = 133 Pa), primarily due to the increase in the oxygen gradient in pericentral regions. Ethanol treatment also increased hepatic oxygen uptake significantly, from 110 to 144 (mumol/g)/hr. Treatment with the antithyroid drug 6-propyl-2-thiouracil reversed the effect of ethanol on O2 uptake and on the lobular oxygen gradient. The oxygen gradients measured with the micro-light guide were confirmed by direct measurement of tissue oxygen tensions in periportal and pericentral areas by using an oxygen electrode. These data are consistent with the hypothesis that chronic treatment with ethanol causes the pericentral region of the liver lobule to become susceptible to hypoxic cellular injury. This may be responsible, at least in part, for the localized hepatotoxic effects of ethanol.
Separation of two thyrotropin binding components from porcine thyroid tissue by affinity chromatography: characterization of high and low affinity sites.
Creators: name: McQuade, R.
name: Nayfeh, S. N.
name: Grunwald, R.
name: Thomas, C. G.
name: Drummond, R. W.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1982

description: Two distinct thyrotropin (TSH) binding species have been separated from solubilized porcine thyroid membranes. Membranes was solubilized with 1% Triton X-100, and the supernatant was recovered by centrifugation at 105,000 X g. Scatchard analysis of thyrotropin binding to solubilized membranes (SM) yielded a nonlinear plot with Kd values for the high and low affinity components similar to those of intact membranes. Chromatography of the SM preparation on concanavalin A-Sepharose 4B resulted in the retention of 10-20% of the binding activity. Upon elution of the column, a peak of binding material (5-7% of total activity) was eluted at 0.3 M alpha-methyl-D-mannoside. This concanavalin A (Con A) bound fraction exhibited a linear Scatchard plot with a Kd value similar to that of the high affinity component of the SM. The protein fraction that did not bind to Con A (Con A unbound) also exhibited a linear Scatchard plot, but with affinity similar to that of the low affinity component of SM. Discontinuous sucrose density gradient ultracentrifugation revealed the presence of two major binding peaks in the solubilized membrane preparation. The slowly sedimenting peak corresponded to that seen in the Con A bound fraction, whereas the rapidly sedimenting peak corresponded to that of the Con A unbound fraction. Sepharose 6B chromatography indicated that in the case of the Con A unbound fraction, a single peak of specific binding activity was eluted in the void volume, and in the case of the Con A bound fraction, one major peak with an approximate Stokes radius of 67 A and several other minor peaks were eluted. These results demonstrate the physical separation of two distinct TSH binding species from thyroid membranes and provide further support for the model of multiple classes of binding sites.
Analysis, design and development of packages - Report R1
Creators: name: Oggioni, Alessandro
name: Bordogna, Gloria
name: Lotti, Alessandro
name: Minelli, Annalisa
name: Sordoni, Silvia
name: Tagliolato, Paolo
name: Babbini, Lorenza
Publisher: name: Zenodo
Year: 2023

description: The 3 proposed solutions for Dashboard, GeoStories and Knowledge Hub carried out under phase 1 of the agreement to analysis, design and development of packages related to the development of the UNEP-MAP Knowledge Platform (KP) are described here.

The ...
Ultrastructure of a hyaluronic acid matrix
Creators: name: Williams, L. D.
name: Nermut, M. V.
name: Hadler, N. M.
name: Dourmashkin, R. R.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1982

description: Freeze-etch replicas of a hylauronic acid matrix were visualized by electron microscopy. In water a coarse branching fibrillar network of hyaluronic acid aggregates was seen. The high solvent permeability of this matrix suggests that the spaces observed are relatively devoid of unaggregated polymer. Addition of calcium disordered the matrix, resulting in a more dispersed felt of polymer.
Stable non-Fermi liquid phase of itinerant spin-orbit coupled ferromagnets
Creators: name: Bahri, Yasaman
name: Potter, Andrew C.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Strongly Correlated Electrons (cond-mat.str-el)
subject: Mesoscale and Nanoscale Physics (cond-mat.mes-hall)
subject: FOS: Physical sciences

description: Direct coupling between gapless bosons and a Fermi surface results in the destruction of Landau quasiparticles and a breakdown of Fermi liquid theory. Such a non-Fermi liquid phase arises in spin-orbit coupled ferromagnets with spontaneously broken continuous symmetries due to strong coupling between rotational Goldstone modes and itinerant electrons. These systems provide an experimentally accessible context for studying non-Fermi liquid physics. Possible examples include low-density Rashba coupled electron gases, which have a natural tendency towards spontaneous ferromagnetism, or topological insulator surface states with proximity-induced ferromagnetism. Crucially, unlike the related case of a spontaneous nematic distortion of the Fermi surface, for which the non-Fermi liquid regime is expected to be masked by a superconducting dome, we show that the non-Fermi liquid phase in spin-orbit coupled ferromagnets is stable.
description: 14 pages; typos fixed and transport/disorder sections revised
In Situ Aerosol Acidity Measurements Using a UV-Visible Micro-Spectrometer and its Application to the Ambient Air
Creators: name: Jang, Myoseon
name: Sun, Shiqi
name: Winslow, Ryan
name: Han, Sanghee
name: Yu, Zechen
Publisher: name: Taylor & Francis
Year: 2020
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Genetics
subject: Pharmacology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: 20199 Astronomical and Space Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: 69999 Biological Sciences not elsewhere classified

description: An in situ analytical method was demonstrated to measure the proton concentration ([H+]C-RUV) of an aerosol particle by using colorimetry integrated with a Reflectance UV-Visible spectrometer (C-RUV). Acidic particles comprising ammonium, sulfate, and water were generated in a flow tube under varying humidity and employed to calibrate the method using the inorganic thermodynamic models (i.e., E-AIM and ISORROPIA). The predictive [H+]C-RUV equation derived using strongly acidic compositions was then extended to ammonia-rich aerosols, which were lacking in the database of the thermodynamic models. The predictive [H+]C-RUV equation was also expanded to aerosols composed of sodium, ammonium, and sulfate. [H+]C-RUV generally agrees with both E-AIM predicted [H+] and ISORROPIA predicted [H+] for highly acidic aerosols, or aerosols at high humidity. For ammonia-rich aerosols under low humidity, [H+]C-RUV disagrees with that predicted from inorganic thermodynamic models. C-RUV was feasible for ambient aerosols because colorimetry is specific to aerosol acidity. Most aerosols collected at the University of Florida between 2018 and 2019 were acidic. Sodium ions appeared during the spring and summer, as coastal sea breezes travelled inland. The concentrations of ammonium and nitrate were high in the winter due to the phase partitioning of nitric acid and ammonia gases. The fraction of non-electrolytic dialkyl-organosulfate (diOS) to total sulfate is estimated by comparing the actual particle [H+] measured by C-RUV to the [H+] predicted using the inorganic composition and the inorganic thermodynamic models. The diOS fraction varied from 0% to 60% and was higher in the summer months when [H+] is high.
Botrocetin (venom coagglutinin): reaction with a broad spectrum of multimeric forms of factor VIII macromolecular complex.
Creators: name: Fricke, W. A.
name: Wagner, R. H.
name: Read, M. S.
name: Brinkhous, K. M.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1983

description: Botrocetin, originally called venom coagglutinin, is a Bothrops factor that causes aggregation of blood platelets in the presence of the von Willebrand component of the factor VIII macromolecular complex. The complex consists of a series of multimers with a molecular weight of about 1-20 x 10(6). Ristocetin, another agent that causes platelet aggregation dependent on von Willebrand factor, reacts with only the higher molecular weight multimers. We report on the reactivity of botrocetin in relation to the multimeric structure of the factor VIII complex. Several plasmas or plasma fractions with abnormal distribution of the multimeric sizes were examined, including variant von Willebrand disease type IIA with lack of the higher molecular weight forms, commercial antihemophilic factor concentrates with a preponderance of lower molecular weight forms, cryoprecipitate-free plasma containing mainly the smaller multimers, and a chromatographic fraction of plasma containing only the highest molecular weight polymers. Factor VIII-related antigen content was adjusted to 25-100%. All of the preparations lacking the high molecular weight forms caused prompt platelet aggregation with botrocetin, but none of them caused aggregation in the ristocetin test made isochronal with the botrocetin test. The very high molecular weight polymers were equally effective with botrocetin and ristocetin. These findings indicate that the Bothrops factor is reactive with a broad spectrum of high to low molecular weight forms of the factor VIII complex, suggesting that bioassays of von Willebrand factor with botrocetin should correlate better with immunoassays for factor VIII-related antigen and could reflect better the full platelet-aggregating function of the complex than do ristocetin determinations.
Calcium/calmodulin inhibition of coupled NaCl transport in membrane vesicles from rabbit ileal brush border.
Creators: name: Powell, D. W.
name: Fan, C. C.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1983

description: The role of Ca2+ and calmodulin in regulating coupled NaCl transport has been investigated in membrane vesicles from rabbit ileal brush border. Uptake of 22Na+ and 36Cl- was determined by a rapid filtration technique in vesicles isolated with a sucrose density gradient ultracentrifugation method. Ca2+ on the inside of the vesicle inhibited Na+ uptake when Cl- was the anion and Cl- uptake when Na+ was the cation by approximately equal to 30%. Ca2+ on the outside had no effect. When gluconate was the anion or when choline was the cation, Na+ or Cl- uptake was reduced by only 9-12%. A similar inhibition of D-[3H]mannitol uptake (10-17%) suggests this was due to a nonspecific decrease in the membrane permeability. Other cations such as Ba2+ and Mg2+ had no effect, but La3+ inhibited Na+ and Cl- uptake to the same degree as Ca2+. Calmodulin (2 microM) in combination with Ca2+ (1 microM, free concentration) significantly inhibited Na+ uptake when Cl- was the anion by 21-32% and Cl- uptake when Na+ was the cation by 20-27%. This effect was completely reversed by 10 microM trifluoperazine. When gluconate was the anion or when choline was the cation, Na+ or Cl- uptake was unaffected by Ca2+/calmodulin and trifluoperazine. The Ki for Ca2+ inhibition of Cl- -coupled Na+ uptake was reduced from 200 microM to 0.2 microM by incubation with 20 microM calmodulin. The Ki for exogenously added calmodulin studied at 1 microM Ca2+ was 0.2 microM. The Ki for trifluoperazine inhibition of the Ca2+/calmodulin response was 3 microM. These results represent compelling evidence for intracellular Ca2+/calmodulin regulation of coupled NaCl transport across the intestinal microvillus membrane. The exact mechanism of this regulation remains to be delineated.
The prognostic implications of radiologic extranodal extension in the lymph nodes of patients with nasopharyngeal cancer
Creators: name: Zhou, Pingting
name: Luo, Yunxiu
name: Wang, Chenxi
name: Wang, Rensheng
Publisher: name: Taylor & Francis
Year: 2024
Subjects: subject: Medicine
subject: Cell Biology
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Marine Biology
subject: Cancer
subject: Science Policy

description: Objective: This study was developed to explore the prognostic relevance of radiologic extranodal extension (rENE) in lymph node-positive nasopharyngeal carcinoma (NPC) patients. Materials and methods: A retrospective review of data from 249 eligible patients with NPC was performed, with magnetic resonance imaging scans being used for rENE grading. The prognostic value of rENE was assessed through univariate and multivariate analyses. Results: Log-rank tests revealed significant differences between patients with and without rENE in terms of overall survival, progression-free survival (PFS) and distant metastasis-free survival (DMFS). G2 and G3 patients tended to exhibit worse PFS and DMFS relative to G0/G1 patients (p < 0.05). Long-term chemotherapy cycles were associated with significant improvements in the PFS and DMFS of G2 and G3 patients. Conclusion: These results suggest that higher rENE grades (G2/G3) are independently associated with worse survival outcomes among NPC patients, with more aggressive treatment strategies potentially affording greater prognostic benefits to these individuals. In this study, the clinical features and prognostic outcomes of nasopharyngeal carcinoma (NPC) patients with radiologic extranodal extension (rENE) were analyzed. Larger lymph nodes (≥3 cm) were found to be more commonly associated with more advanced rENE grading (G2 and G3). Significant correlations were observed between rENE and higher Epstein–Barr’s virus-DNA levels, lymph node necrosis and larger metastatic cervical lymph nodes. Rates of complete remission rates at the end of treatment and 3 months postradiotherapy declined with rising rENE grades. Univariate analyses indicated that patients with and without rENE exhibited significant differences in overall survival, progression-free survival (PFS) and distant metastasis-free survival (DMFS) rates. Multivariate analyses indicated that rENE was independently associated with both PFS and DMFS in NPC patients, but not with overall survival. Survival analyses revealed that patients with rENE tended to exhibit poorer outcomes, particularly for patients exhibiting higher rENE grades. In patients with high rENE grades (G2/G3), long-term chemotherapy cycles (≥4) were associated with significant improvements in PFS and DMFS, whereas no similar differences were observed in patients with low rENE grades (G0/G1).
Calcium/calmodulin-dependent phosphorylation of vimentin in rat sertoli cells.
Creators: name: Tres, L. L.
name: Spruill, W. A.
name: Kierszenbaum, A. L.
name: Zysk, J. R.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1983

description: Ca2+-dependent protein phosphorylation and the role of calmodulin in this process was investigated in subcellular fractions of primary cultures of rat Sertoli cells. Significant Ca2+/calmodulin-dependent protein phosphorylation in Sertoli cells was restricted to the cytosol fraction. The calmodulin dependence of these effects was confirmed by using the calmodulin inhibitor trifluoperazine. One of the Ca2+/calmodulin-dependent phosphoproteins was identified as the intermediate filament protein vimentin, based on the following criteria: (i) migration pattern in two-dimensional polyacrylamide gels, (ii) Ca2+/calmodulin-dependent phosphorylation of a 58-kilodalton protein present in detergent-insoluble intermediate filament protein extract of Sertoli cells, and (iii) peptide mapping of the phosphoprotein. These data support a role for Ca2+/calmodulin-dependent protein phosphorylation in the modulation of Sertoli cell cytoskeletal components.