cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Additional file 12 of A facile and scalable in production non-viral gene engineered mesenchymal stem cells for effective suppression of temozolomide-resistant (TMZR) glioblastoma growth
Creators: name: Tu, Geraldine Xue En
name: Ho, Yoon Khei
name: Ng, Zhi Xu
name: Teo, Ke Jia
name: Yeo, Tseng Tsai
name: Heng-Phon Too
Publisher: name: figshare
Year: 2020
Subjects: subject: Biophysics
subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Molecular Biology
subject: Pharmacology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Cancer
subject: Hematology
subject: Plant Biology
subject: 60506 Virology

description: Additional file 12. Coculture duration and 5FC concentration required for optimal in vitro cytotoxicity study. a CD::UPRT::GFP_AD-MSCs were cultured with U-251MG in low serum DMEM, in the presence or absence of 100 μg/mL 5FC. With pre-seeded U-251MG, the therapeutic cells were added at ratios of 1 CD::UPRT::GFP_AD-MSCs to 10, 5, 1 cancer cells. Three, five and seven days later (D3, D5, D7), cell viability in the treatment conditions was evaluated spectrophotometrically by MTS assay. Cell viability was defined as sample/control × 100%. Conditions without 5FC treatment served as controls for respective AD-MSCs to U-251MG ratios. Data of biological quadruplicates were expressed as mean + SD. b Similar study was performed to determine the 5FC concentration required for maximal cytotoxic effect. The coculture of CD::UPRT::GFP_AD-MSCs and U-251MG cells were treated with various concentration of 5FC for 7 days. At the end of the experiment, MTS assay was performed. Data of biological quadruplicates are expressed as mean + SD. Significant differences between 50 μg/mL of 5FC and other 5FC concentrations were calculated using two tailed Student’s t-test. *, P
Synthetic and Mechanistic Studies in Ruthenium-catalyzed Olefin Metathesis
Creators: name: Reckling, Amy
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2013
Subjects: Ruthenium catalysts
Boomerang mechanism
Free carbene
Metathesis
Clean synthesis

description: Ruthenium - catalyzed olefin metathesis is now an invaluable tool in organic synthesis. However, routes to the dominant metathesis catalysts, the second - generation Grubbs and Hoveyda catalysts (RuCl 2 (PCy 3 )(H 2 IMes)(=CHPh) and RuCl 2 (H 2 IMes)[= CH( o - O i Pr)C 6 H 4 ], respectively) are plagued with problems. The common reliance on in situ methods to generate the N - heterocyclic carbene H 2 IMes severely limits stoichiometric control, and results in contamination by byproducts, some of which are readily overlooked, and some of which are difficult to remove. Both can affect batch - to - batch reproducibility in catalysis. This thesis work demonstrated that widespread perceptions of the instability of free H 2 IMes are erroneous, and that the free carbene is readily handled under water - free conditions. Clean, convenient, near - quantitative routes were developed to these second - generation catalysts by ligand exchange of their first - gen eration counterparts RuCl 2 (PCy 3 ) 2 (=CHPh), RuCl 2 (PCy 3 )[= CH( o - O i Pr)C 6 H 4 ] with free H 2 IMes, with sequestration of the liberated phosphine by an ion - exchange resin. A second focus was examination of a much - debated hypothesis in olefin metathesis: that is, the extent to which the high productivity of the Hoveyda catalysts reflects re - uptake of the styrenyl ether functionality released in the initial cycle of metathesis. Current evidence for and against this "boomerang" hypothesis is critically examined, and new approaches to examining its operation are described. Specifically, the rate of decomposition, vs. re - uptake, is examined for the active species RuCl 2 (PCy 3 )(=CH 2 ), and background exchange of the parent catalyst with free styrenyl ether is measured by use of a 13 C - labelled styrenyl ether. These studies confirm the relevance of the boomerang mechanism for first - generation Hoveyda catalysts.
The categories for the running of the model from GIS-ODE: Linking dynamic population models with GIS to predict pathogen vector abundance across a country under climate change scenarios
Creators: name: Worton, Adrian
name: Norman, Rachel
name: Gilbert, Lucy
name: Porter, Ros
Publisher: name: The Royal Society
Year: 2024
Subjects: classificationCode: 490102
subject: Biological mathematics

description: The 3km × 3km cells were firstly split by habitat type, based on the UK Land Cover Map 2000. Upland moorland cells were split according to their deer densities, according to average historical Deer Management Group counts, with the third with the lowest density placed in the high grouse/no deer group, the highest third placed in the no grouse/high deer group, and the remaining third in the medium grouse/medium deer group. Finally, the cells within each habitat were split into temperature groups, based on Met Office averages for 1971-2000, divided so there were approximately 100 cells in each group.
Charismatic Healing: A Phenomenological Study of Spiritual Healing in Ottawa, Canada
Creators: name: Pohran, Nadya
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2015
Subjects: Spiritual healing
Charismatic
Protestant
Christian
lived religion
phenomenology
cultural anthropology
ethnography
Ottawa, Canada

description: Spiritual healing is a ubiquitous and fundamental part of Charismatic Christianity; it is indelibly linked to understandings of God, society, and individual identity. And yet, the phenomenon of spiritual healing—particularly its expression within North American, Abrahamic traditions—has been understudied within academia. In this thesis, I take a phenomenological approach in order to better comprehend the meaning-making process behind spiritual healing rituals amongst Charismatic Protestant Christians in Ottawa, Canada. Through a small-scale, local ethnographic study in Ottawa in which I conducted participant observation and several in-depth interviews, I explore Charismatic Christianity through the lens of lived religion. Based on a series of focused case studies, I conclude that the Charismatic cosmological worldview (one in which cosmic-wide restoration is emphasised) correlates with, and contributes to, the Charismatic emphasis on individual healing.
Neue Fördermöglichkeiten zur optimierten Gasfassung und Deponiebelüftung im Rahmen der Nationalen Klimaschutzinitiative
Creators: name: Butz, Wolfgang
Publisher: name: My University
Year: 2019

description: Seit 1990 konnten in Deutschland die jährlichen Methanemissionen aus Deponien durch rechtliche Anforderungen sowie abfallwirtschaftliche und deponietechnische Maßnahmen um ca. 80% gemindert werden. Trotz dieser Erfolge emittieren die Deponien noch immer etwa 300.000 Tonnen Methan pro Jahr (~7,5 Mio. t/a Co2-Äquivalente), sodass hier noch erhebliche Minderungspotenziale bestehen. Seit 2013 fördert das Bundesministerium (BMU) Investitionen zur Minderung der Deponieemissionen durch die Nationale Klimaschutzinitiative (NKI). Im zusammenhang mit dem Klimaschutzgesetz hat das BMU die Förderung von NKI-Deponieprojekten intensiviert und um zusätzliche Förderschwerpunkte erweitert. Quelle: Bioabfall- und stoffspezifische Verwertung III / Witzenhausen-Institut ; Herausgeber: Prof. Dr.-Ing. Klaus Wiemer [und zwei weitere]. - 1. Auflage. - Witzenhausen - (2019), Seiten 419
Additional file 3 of A facile and scalable in production non-viral gene engineered mesenchymal stem cells for effective suppression of temozolomide-resistant (TMZR) glioblastoma growth
Creators: name: Tu, Geraldine Xue En
name: Ho, Yoon Khei
name: Ng, Zhi Xu
name: Teo, Ke Jia
name: Yeo, Tseng Tsai
name: Heng-Phon Too
Publisher: name: figshare
Year: 2020
Subjects: subject: Biophysics
subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Molecular Biology
subject: Pharmacology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Cancer
subject: Hematology
subject: Plant Biology
subject: 60506 Virology

description: Additional file 3. Gene expression of genes related in 5FU resistance pathway. Fold change of expression for DPYD, TYMS and UMPS in a U-251MG and b U-87MG TMRZ cell lines were calculated in relative to their respective parental cell lines. Similarly, changes in the expression of ABCC5 transporter were calculated accordingly for c U-251MG and d U-87MG TMZR cell lines. All samples were analysed in triplicates. The fold change in the expressions of the gene of interest was calculated after normalization to the house keeping gene GAPDH. Line graph shows the average fold change in gene expression, mean + SD (n = 3). Significant differences between parental and TMZR cells were calculated using unpaired, two-tailed Student’s t-test. p-value
Development and Usability Testing of a Neonatal Intensive Care Unit Physician-Parent Decision Support Tool (PPADS)
Creators: name: Weyand, Sabine A
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2011
Subjects: Clinical decision support
Neonatal intensive care unit
Parent decision support
Decision support tool
Usability testing

description: This thesis presents the development and evaluation of a computerized physician-parent decision support tool for a neonatal intensive care unit (NICU), known as Physician and Parent Decision Support (PPADS). The NICU is a specialized hospital unit that treats very-ill neonates. Many difficult care decisions are made daily for this vulnerable population. The PPADS tool aims to augment current NICU decision-making by helping parents and physicians make more informed decisions, improving physician-parent communication, increasing parent decision-making satisfaction, decreasing conflict, and increasing decision efficiency. The development of the PPADS tool followed a five-step methodology: assessing the clinical environment, establishing the design criteria, developing the system design, implementing the system, and performing usability testing. Usability testing of the PPADS tool was performed on neonatologists and on parents of neonates who have graduated (survived) from a tertiary level NICU. The usability testing demonstrated the usefulness and ease of use of the tool.
An innovative linkage of curricula design, power engineering industries and universities in Queensland Australia to promote engineering education
Creators: name: Hosseinzadeh, Nasser
name: Senini, Steven
Publisher: name: Swinburne
Year: 2024

description: The demand for engineers and their educators has been continuously growing in Australia. In recent years, the academia and industry leaders have proposed and implemented a number of strategies to meet the demand. One of these strategies in the State of Queensland has been the formation of Power Engineering Alliance (PEA), which brings together Industry and University Partners with a common interest in education, research and professional development associated with Power Engineering. The Objectives of the PEA are to boost the quality and number of engineering graduates with the skills and motivation for a career in power engineering, and to contribute to their ongoing professional development. Central Queensland University (CQU) has been one of the leading University Partners to this alliance. CQU has been recognised as a provider of engineering teaching excellence to the Central Queensland region and beyond.
Additional file 13 of A facile and scalable in production non-viral gene engineered mesenchymal stem cells for effective suppression of temozolomide-resistant (TMZR) glioblastoma growth
Creators: name: Tu, Geraldine Xue En
name: Ho, Yoon Khei
name: Ng, Zhi Xu
name: Teo, Ke Jia
name: Yeo, Tseng Tsai
name: Heng-Phon Too
Publisher: name: figshare
Year: 2020
Subjects: subject: Biophysics
subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Molecular Biology
subject: Pharmacology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Cancer
subject: Hematology
subject: Plant Biology
subject: 60506 Virology

description: Additional file 13. Unmodified AD-MSCs were seeded in 24-well plate at 2500/cm2. One day later, the culture media is replaced with low serum DMEM, in the presence or absence of 100 μg/mL 5FC. Cells were further incubated for 4 days. At the end of experiment, cells were trypsinised and subjected to cell count with the automated cell counter NC-3000. Graph bar presents the absolute cell number harvested from triplicates of each condition (mean + SD).
Collaboration: the key towards a resource resilient society
Creators: name: Vasseur, Karolien
name: Campforts, Mieke
name: Van Camp, Maurits
Publisher: name: Swinburne
Year: 2024

description: The global demand for technology materials is continuously increasing as the world's population grows and high standards of living are sought in developing and transition countries. To secure a reliable and sustainable supply of these metals, innovative solutions need to be developed along the entire value chain. This requires a system-wide, collaborative approach focusing on sustainable mining methods, substitution of critical metals and recovery of metals from secondary sources.
The Perfect Approach to Adverbs: Applying Variation Theory to Competing Models
Creators: name: Roy, Joseph
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2014
Subjects: present perfect
adverbs
Canadian English
Early Modern English
Statistics in Sociolinguistics
logistic mixed effects model
Goldvarb X

description: The question of adverbs and the meaning of the present perfect across varieties of English is central to sociolinguistic variationist methodologies that have approached the study of the present perfect (Winford, 1993; Tagliamonte, 1997; van Herk, 2008, 2010; Davydova, 2010; Tagliamonte, 2013). This dissertation attempts to disentangle the effect of adverbial support from the three canonical readings of the present perfect (Resultative, Experiential and Continuative). Canadian English, an understudied variety of English, is used to situate the results seen in the Early Modern English data. Early Modern English reflects the time period in which English has acquired the full modern use of the present perfect with the three readings. In order to address both these questions and current controversies over statistical models in sociolinguistics, different statistical models are used: both the traditional Goldvarb X (Sankoff, Tagliamonte and Smith, 2005) and the newer mixed-effects logistic regression (Johnson, 2009). What is missing from the previous literature in sociolinguistics that advocates logistic mixed-effects models, and provided in this dissertation, is a clear statement of where they are inappropriate to use and their limitations. The rate of adverbial marking of the present perfect in Canadian English falls between rates reported for US and British English in previous studies. The data show in both time periods that while adverbs are highly favored in continuative contexts, they are strongly disfavored in experiential and resultative contexts. In Early Modern English, adverbial support functions statistically differently for resultatives and experientials, but that difference collapses in the Canadian English sample. Both this and the other linguistic contexts support a different analysis for each set of data with respect to adverbial independence from the meaning of the present perfect form. Finally, when the focus of the analysis is on linguistic rather than social factors, both the traditional and newer models provide similar results. Where there are differences, however, these can be accounted for by the number of tokens and different estimation techniques for each model.
The Correlation Analysis of Futures Pricing Mechanism in China’s Carbon Financial Market
Creators: name: Sheng, Chunguang
name: Wang, Guangyu
name: Geng, Yude
name: Chen, Lirong
Publisher: name: Multidisciplinary Digital Publishing Institute
Year: 2020

description: China, taking the concept of sustainable development as the premise, puts forward Intended Nationally Determined Contributions (INDC) to reduce the greenhouse gas emissions in response to climate change. In this context, with the purpose of seeking solutions to a carbon financial market pricing mechanism to build China’s carbon finance market actively and thus achieving the goal of sustainable development, this paper, based on the autoregressive integrated moving average (ARIMA) model, established a carbon price prediction model for the carbon financial market, and studied the relationship between Certified Emission Reduction (CER) futures prices and spot prices, as well as the relationship between European Union allowances (EUA) futures prices and CER futures prices in an empirical manner. In this paper, EUA and CER futures prices of the European Climate Exchange (ECX) and EUA and CER spot prices of the BlueNext Environmental Exchange were selected as research objects. Granger causality test, co-integration test, and ECM were used to form a progressive econometric analysis framework. The results show that firstly, the ARIMA model can effectively predict carbon futures prices; secondly, CER futures prices cannot guide spot price, and the futures pricing function does not play a role in this market; thirdly, EUA futures price can, in the short term, effectively guide the trend of CER futures prices, with the futures pricing function between the two markets. In the long run, however, the future pricing function of the two markets is not obvious. Therefore, great differences among maturity of the two markets, degree of policy influence, and market share lead to the failure of long-run futures pricing functions.
Exploring the Role of Corporate Social Responsibility and its Influence on Branding: A Grounded Theory Case Study of Hydro Ottawa
Creators: name: Phillips, Michele
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2015
Subjects: Corporate social responsibility
CSR
Branding
Stakeholder
Grounded Theory
Case Study
Hydro Ottawa
Energy
Stakeholder Theory
Extended Stakeholder Theory

description: The following research examined the role of corporate social responsibility (CSR) and its influence on branding in the energy sector. This research took place at Hydro Ottawa because it is a power distribution and a power generation company, with a mixed private and public business model operating in a monopolistic context, which is a well-rounded example that can be transferred to other organizations and other sectors. This research followed a case study approach guided by Strauss and Corbin’s (1990, 1998) grounded theory. This study attempted to answer the following research question 1) Who are the main stakeholders at Hydro Ottawa and what type of influence do they have on the development of their CSR and branding strategies? 2) How is CSR enunciated and applied at Hydro Ottawa? 3) How does CSR shape the brand image of Hydro Ottawa? In order to explore the research questions, the data collected for this study consisted of interviews, organizational documents, as well as observation. Due to the time limitation for a master’s thesis, six participants were chosen for interviews, which were later transcribed, coded and analysed by the researcher. The findings indicated that building a CSR strategy based on a healthy organizational culture, stakeholder needs, and the triple bottom line (financial, social, environmental) led to CSR essentially becoming the brand. The brand can then be communicated and shaped from and inside-out (from the employees to the external stakeholders)/outside-in (from external stakeholders to inside the organization) approach. Based on a grounded theory analysis, the researcher created a step-by-step prescriptive model that could be used to advance corporate social responsibility into Hydro Ottawa’s brand as well as offer a critical perspective of the findings.
Clinical Prediction Rule for Treatment Change Based on Echocardiogram Findings in Transient Ischemic Attack and Non-Disabling Stroke
Creators: name: Alsadoon, Abdulaziz
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2015
Subjects: Echocardiogram
Cardiac ultrasound
Prediction rule
Decision rule
Transient ischemic attack
Neurology
Treatment change
Stroke

description: The goal of this study was to derive a clinical prediction rule for transient ischemic attack (TIA) and non-disabling stroke to predict a treatment change based on echocardiogram. Methods: We conducted a cohort sub-study for TIA and non-disabling stroke patients collected over five years from 8 Emergency Departments. We compiled a list of 27 potential predictors to look for treatment change based on echocardiogram findings. We used a univariate, logistic regression and recursive partitioning analysis to develop the final prediction model. Results: The frequency of treatment change was seen in 87 (3.1%) of 2804 cases. The final model contains six predictors: age less than 50 years old, coronary artery disease history, history of heart failure, any language deficit, posterior circulation infarct and middle cerebral artery infarct on neuroimaging. Conclusions: We have developed a highly sensitive clinic prediction rule to guide in the use of echocardiogram in TIA and non-disabling stroke.
Additional file 4 of A facile and scalable in production non-viral gene engineered mesenchymal stem cells for effective suppression of temozolomide-resistant (TMZR) glioblastoma growth
Creators: name: Tu, Geraldine Xue En
name: Ho, Yoon Khei
name: Ng, Zhi Xu
name: Teo, Ke Jia
name: Yeo, Tseng Tsai
name: Heng-Phon Too
Publisher: name: figshare
Year: 2020
Subjects: subject: Biophysics
subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Molecular Biology
subject: Pharmacology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Cancer
subject: Hematology
subject: Plant Biology
subject: 60506 Virology

description: Additional file 4. Gene expression z-score of patient derived cell lines for 5-FU pathway. DPYD, TYMS, UMPS, TYMP and ABCC5 expression levels are shown. The information was obtained from www.hgcc.se Dated: 3 Jun 2019.
The Pacification of Favelas of Rio de Janeiro: A Neoliberal Twist to an Old-Fashioned Intervention
Creators: name: Pier Angelli, De Luca Maciel
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2015
Subjects: Pacification
Security
Neoliberalism
Marginalized areas
Urban Renewal
Militarization
Favelas
Police power

description: In 2008, a policy to address the territorialisation of the drug trade in Rio de Janeiro’s favelas was developed: the pacification of favelas. It involves two key elements, policing and local development, which are said to work in tandem to conquer the territory from drug dealers and integrate these areas to the city. Drawing from the literature on the governance of marginalized areas and neoliberal practices of security, this study explores how the pacification of favelas unfolds within a neoliberal context. The findings of this study are based on a thematic analysis of twenty-five in-depth interviews with key actors involved in the development and implementation of three core projects of the policy (the Pacifying Police Unit and two projects that coordinates social efforts, UPP Social and Peace Territories). Using the theoretical lenses of pacification proposed by Neocleous (2011) and Rigakos (2011), this research argues that the pacification of favelas can be considered a "project of pacification" (Rigakos, 2011). The analysis demonstrates that the dual strategy of the policy reinforces neoliberal practices to govern through fear, resulting in the militarization of favelas. Moreover, this study also finds that this policy serves capitalist interests when implemented within a neoliberal framework, even though its core elements resemble interventionist initiatives of colonial enterprises. My findings also reveal that some participants resist the implementation of initiatives based on a neoliberal framework. However, although their actions seek to emancipate and build an ethical community in favelas, based on long-term and fraternal bonds, the mainstream approach is still prevalent. As a result, favelas are progressively turned into aesthetic communities in which the interests of the market are the driving force.
ЯЗЫКОВАЯ ПОЛИТИКА В СУДОПРОИЗВОДСТВЕ: ИСПОЛЬЗОВАНИЕ УЗБЕКСКОГО, РУССКОГО ЯЗЫКОВ И ЯЗЫКОВ МЕНЬШИНСТВ В СУДАХ И ОФИЦИАЛЬНЫХ ДОКУМЕНТАХ УЗБЕКИСТАНА
Creators: name: Очилов, Аслиддин
Publisher: name: Zenodo
Year: 2024

description: В данной статье рассматривается сложный языковой ландшафт правовой системы Узбекистана, уделяя особое внимание использованию узбекского, русского и языков меньшинств в судах и официальных документах. В нем исследуются исторический контекст, действующая законодательная база и практическая реализация языковой политики в судопроизводстве страны. В исследовании анализируются проблемы и возможности, предоставляемые многоязычием в правовой сфере, с учетом аспектов доступа к правосудию, культурных прав и национальной идентичности. Благодаря всестороннему обзору законодательства, тематическим исследованиям и сравнительному анализу это исследование вносит вклад в более широкий дискурс о языковых правах и правовом плюрализме в постсоветской Центральной Азии.
Additional file 14 of A facile and scalable in production non-viral gene engineered mesenchymal stem cells for effective suppression of temozolomide-resistant (TMZR) glioblastoma growth
Creators: name: Tu, Geraldine Xue En
name: Ho, Yoon Khei
name: Ng, Zhi Xu
name: Teo, Ke Jia
name: Yeo, Tseng Tsai
name: Heng-Phon Too
Publisher: name: figshare
Year: 2020
Subjects: subject: Biophysics
subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Molecular Biology
subject: Pharmacology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Cancer
subject: Hematology
subject: Plant Biology
subject: 60506 Virology

description: Additional file 14. AD-MSCs generated with PEI plus Enhancers outperformed other methods. AD-MSCs were transfected with CD::UPRT::GFP plasmid using various protocols. Twenty-four hours post-transfection, CD::UPRT::GFP_AD-MSCs were cocultured with U-251MG in DMEM supplemented with 2% FBS, in the presence or absence of 100 μg/mL 5FC. The therapeutic cells were mixed at ratios of 1 CD::UPRT::GFP_AD-MSC to 25, 10, 5, 1 cancer cells. Seven days later, cell viability in the treatment conditions was evaluated spectrophotometrically by MTS assay. Cell viability was defined as sample/control × 100%. Conditions without 5FC treatment served as controls. No MSC condition suggests lack of 5FC cytotoxicity in cancer cells. Data presents mean + SD of the biological quadruplicates. Statistical differences between Lipofectamine 3000 and other methods were calculated using two tailed Student’s t-test. **, P
Additional file 2 of A facile and scalable in production non-viral gene engineered mesenchymal stem cells for effective suppression of temozolomide-resistant (TMZR) glioblastoma growth
Creators: name: Tu, Geraldine Xue En
name: Ho, Yoon Khei
name: Ng, Zhi Xu
name: Teo, Ke Jia
name: Yeo, Tseng Tsai
name: Heng-Phon Too
Publisher: name: figshare
Year: 2020
Subjects: subject: Biophysics
subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Molecular Biology
subject: Pharmacology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Cancer
subject: Hematology
subject: Plant Biology
subject: 60506 Virology

description: Additional file 2. Flow cytometry profiles of the cell cycle of the TMZ sensitive and resistant cell lines in the presence and absence of TMZ treatment. The number of cells acquired during flow cytometry measurement is 5000 per sample. The analysis of the cell cycle is presented in Fig. 1c and d.
Inter-Organizational Problem Solving Among Disaster Managers: The Role of Common Ground
Creators: name: Blust-Volpato, Stephanie Anna
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2014
Subjects: Common Ground
Shared Mental Models
Disaster Management
Collaboration
Problem Solving
Inter-Organizational
Emergency Management
Coordination

description: Despite disaster managers’ best efforts, inter-organizational disaster management suffers from varying levels of success. One factor that is likely to account for these variations is team mutual understanding, also known as common ground. To validate the potential effect of common ground in disaster management, the thesis investigates common ground in several interviews with disaster managers and in an experimental study involving an inter-organizational disaster event scenario. Analysis of interviews revealed that disaster managers perceived gaps in understanding between responders, the importance of mutual understanding, and perceived common ground similarly to depictions in theory with a few exceptions. Analyses of the experimental study indicated that contextual factors of Team composition, Problem solving approach and Type of tasks differently impacted measures of performance and implicit coordination, and that implicit coordination partially mediated and supressed the relationship between contextual factors and decision quality. Findings suggest the variation in disaster managers’ performance can be ascribed to common ground, implicit coordination, and contextual factors. Moreover, results showed the satisfaction with outcome did not correlate with expert rated quality of decision; and that while satisfaction related to consensus and quality of the decision was linked to generating alternative ideas and debate. Collaboration proved to be more effective in public communication tasks, especially for homogenous team composition. The findings support initiatives for more cross-training and further lab and field experiments.
Additional file 6 of A facile and scalable in production non-viral gene engineered mesenchymal stem cells for effective suppression of temozolomide-resistant (TMZR) glioblastoma growth
Creators: name: Tu, Geraldine Xue En
name: Ho, Yoon Khei
name: Ng, Zhi Xu
name: Teo, Ke Jia
name: Yeo, Tseng Tsai
name: Heng-Phon Too
Publisher: name: figshare
Year: 2020
Subjects: subject: Biophysics
subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Molecular Biology
subject: Pharmacology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Cancer
subject: Hematology
subject: Plant Biology
subject: 60506 Virology

description: Additional file 6. Supplementation of Enhancer to improve transfection in AD-MSCs. One day post seeding of 50,000 AD-MSCs, cells were transfected by 150-500 ng of PF463-CMV-GFP complexed with PEI at 1 μg pDNA to 3 μL PEI, in the presence or absence of Enhancer. a Two days post-transfection, the fluorescent images were captured at 4x magnification. Representative images are presented. b After which, cells were harvested for FACS analysis. Untransfected MSC served as negative control for gating. Data represents mean ± SD, n = 3 (Bar graph).
Jews and the English Nation: An Intertextual Approach to Evolving Representations of Jews in British Fiction, 1701-1876
Creators: name: Kaiserman, Aaron Samuel
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2016
Subjects: British Literature
Jews
Jewish Representation

description: Recent scholarship on the representations of Jews in British Romantic fiction has explored the relationship between the radical changes in Jewish characterization of the period and shifting cultural values. Judith Page, for example, considers the effect of Romantic notions of sentiment, detailing especially how Jews test the limits of sympathetic feeling, and Michael Ragussis has linked the surge of interest in Jews to their value as rhetorically useful subjects in relation to debates surrounding English and British identity. Such studies at times draw attention to the impact of older characterizations of Jews on the new, typically to reinforce claims that relate changing Jewish portrayals to particular cultural and historical developments. Yet, the impact of literary precedent itself has not been fully considered as a leading factor in inspiring new ideas about Jewish characterization. This study takes as its centrepiece the development of the sympathetic or benevolent Jew in the Romantic period, best characterized by Richard Cumberland’s sentimental comedy The Jew (1794), and the historical novels Harrington (1814), and Ivanhoe (1819) by Maria Edgeworth and Walter Scott respectively. These works draw heavily on pre-existing Jewish-themed texts, notably Shakespeare’s The Merchant of Venice (1598). While the play’s Jewish villain Shylock exerts a powerful and well-documented influence on later Jewish characters, the relevance of these Shylockian imitators merits more minute investigation in terms of their impact on the gradual transformation of ideas about Jews in fiction. For this reason, this dissertation takes a long period of history as its subject in order to emphasize that innovation in Jewish portrayal results not from ongoing social change alone, but equally from the interplay of past literary influences and developments in style and genre.
Additional file 7 of A facile and scalable in production non-viral gene engineered mesenchymal stem cells for effective suppression of temozolomide-resistant (TMZR) glioblastoma growth
Creators: name: Tu, Geraldine Xue En
name: Ho, Yoon Khei
name: Ng, Zhi Xu
name: Teo, Ke Jia
name: Yeo, Tseng Tsai
name: Heng-Phon Too
Publisher: name: figshare
Year: 2020
Subjects: subject: Biophysics
subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Molecular Biology
subject: Pharmacology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Cancer
subject: Hematology
subject: Plant Biology
subject: 60506 Virology

description: Additional file 7. Scaling out MSC modification process on flat-bed culture. AD-MSCs were seeded in 6-well plate, T25, T75 and T175 flasks at 20,000/cm2. One day post seeding, cells were transfected with 150 ng PF463-CMV-GFP/ cm2 complexed at 1 μg pDNA to 3 μL PEI. Two-day post transfection, cells were harvested for analysis. Total number of cells harvested from each culture vessels were determined by the automated cell counter NC-3000. After which, the % of GFP+ cells measured with flow cytometry. a The scatter plot represents absolute number of GFP+ cells collected from each culture vessel; deduced from the readout of cell counting and flow cytometry of the GFP expression. Data of biological triplicates are expressed as mean + SD. b Representative images present the flow cytometry profile of unmodified and modified AD-MSCs harvested from T175 flasks.
Dioxine und dioxinähnliche PCB in Umwelt und Nahrungsketten
Creators: name: Behnke, Anja
name: Blondzik, Katrin
name: Böhnhardt, Anna
name: Bussian, Bernd M.
name: Dauert, Ute
name: Fettig, Ina
name: Gärtner, Philipp
name: Giese, Evelyn
name: Juhrich, Kristina
name: Knetsch, Gerlinde
name: Konietzka, Rainer
name: Koschorreck, Jan
name: Markard, Christiane
name: Minkos, Andrea
name: Moriske, Heinz-Jörn
name: Plickert, Sebastian
name: Rappolder, Marianne
name: Schmidt, Simone
name: Utermann, Jens
name: Schröter-Kermani, Christa
Publisher: name: My University
Year: 2017
Subjects: subject: Dioxine
subject: Koplanare PCB
subject: Polychlorierte Biphenyle
subject: Dioxinähnliche PCB
subject: Furane
subject: dl-PCB
subject: PCB
subject: Dioxinähnliche polychlorierte Biphenyle
subject: Chemikalien

description: Dioxine und dioxinähnliche polychlorierte Biphenyle (dl-PCB) gehören zu den gefährlichsten Umweltgiften. Obwohl die Dioxin-Emissionen seit 1990 in Deutschland und Europa deutlich verringert und der Einsatz von PCB 1989 verboten wurde, kommen Dioxine und PCB aufgrund ihrer Langlebigkeit noch immer in der Luft, im Boden oder in Gewässersedimenten vor und gelangen so in die Nahrungskette von Tier und Mensch. Um das zu vermeiden, ist es notwendig, alle Quellen aufzuspüren und die Belastungen der Umwelt weiter zu minimieren. Das aktualisierte Hintergrundpapier des Umweltbundesamtes informiert ausführlich über die Zusammenhänge zwischen der Belastung von Umwelt und tierischen Nahrungsmitteln durch Dioxine und dl-PCB - von der Entstehung über den Transfer in die Nahrungsketten bis zu den Wirkungen auf den Menschen. Quelle: https://www.umweltbundesamt.de
description: ⁠Dioxine⁠ und dioxinähnliche polychlorierte Biphenyle (⁠dl-PCB⁠) gehören zu den gefährlichsten Umweltgiften. Obwohl die Dioxin-Emissionen seit 1990 in Deutschland und Europa deutlich verringert und der Einsatz von PCB 1989 verboten wurde, kommen Dioxine und PCB aufgrund ihrer Langlebigkeit noch immer in der Luft, im Boden oder in Gewässersedimenten vor und gelangen so in die Nahrungskette von Tier und Mensch. Um das zu vermeiden, ist es notwendig, alle Quellen aufzuspüren und die Belastungen der Umwelt weiter zu minimieren. Das aktualisierte Hintergrundpapier des Umweltbundesamtes informiert ausführlich über die Zusammenhänge zwischen der Belastung von Umwelt und tierischen Nahrungsmitteln durch Dioxine und dl-PCB - von der Entstehung über den Transfer in die Nahrungsketten bis zu den Wirkungen auf den Menschen.
Les Zones franches d'exportation au carrefour entre les politiques d'exception et les cycles d'accumulation du capital
Creators: name: Falaise, Cynthia
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2015
Subjects: Zones franches d'exportation
État d'exception
El Salvador
Spatial fix
David Harvey
Giorgio Agamben
Capitalisme
Néolibéralisme
Amérique latine
IBC
USAID
Zones franches
Export processing zone
EPZ
Salvador

description: En érigeant un cadre théorique réunissant les concepts de fixation spatiale et d’état d’exception, la présente thèse vise à brosser un portrait des Zones franches d’exportation (ZF) à la lumière du cas salvadorien entre les années 1980 et 2000. L’objectif est de proposer des pistes de réflexion quant au caractère exceptionnel des ZF et leur insertion dans les cycles d’accumulation capitalistes. Sont ainsi abordés les discours légitimant la création des ZF et leur maintien, les moyens financiers, juridiques et logistiques déployés pour permettre leur construction, les relations de pouvoir exceptionnelles qui caractérisent les rapports intra et extra-ZF ainsi que les intérêts que les ZF servent. Cette thèse se veut donc une tentative de compréhension du processus général à l’oeuvre derrière les ZF, dans l’objectif de mieux cibler les possibilités de résistance. *** This thesis aims to analyse the emergence of export processing zones (EPZ) through a theoretical framework that combines the concepts of spatial fix and state of exception. Taking as an illustration the Salvadoran case in the 1980-2000 period, the objective is to suggest means for reflecting on the exceptional character of the EPZ and their insertion in cycles of capitalist accumulation. The thesis will also explore the discourses legitimizing the creation and the retention of EPZ, the logistical, juridical and financial means deployed to allow their construction, the exceptional power relations that characterize the intra and extra-EPZ relations and the interests served by EPZ. This thesis is thus an attempt to encompass the general process behind the EPZ, in order to identify the resistance possibilities.
The mere exposure effect: application to emotionally-laden stimuli
Creators: name: Aimers, Nicole Louise
Publisher: name: Swinburne
Year: 2024

description: The mere exposure effect refers to the observation that repeated, unreinforced exposure to a stimulus increases affective evaluations of that stimulus. Nicole applied the theory of mere exposure to cigarette health warnings and identified that liking ratings of high sensation seeking individuals initially increased with repeated exposure. In addition, for both low and high sensation seekers, the health warning images became more pleasant and less arousing over the course of exposure. These findings were problematic, especially because high sensation seekers are more likely to engage in the behaviour that the warnings are designed to eliminate. Nicole’s research called into question the reliance on the health warning images as an effective medium for communicating the detrimental effects of smoking.
The Ubuntu approach to teaching systems development: report on a South African study in progress
Creators: name: Pretorius, J. H. O.
name: Scheepers, H.
Publisher: name: Swinburne
Year: 2024

description: This paper looks at the problem of teaching an undergraduate systems development course which would enable students to develop systems which are functionally 'correct', and to be socially sensitive to the impact of such systems on users and the organization.
Understanding the Pathophysiology of Spinal Muscular Atrophy Skeletal Muscle
Creators: name: Boyer, Justin
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2013
Subjects: Spinal muscular atrophy
myogenesis
skeletal muscle

description: The disruption of the survival motor neuron (SMN1) gene leads to the children’s genetic disease spinal muscular atrophy (SMA). SMA is characterized by the degeneration of α-motor neurons and skeletal muscle atrophy. Although SMA is primarily considered a motor neuron disease, the involvement of muscle in its pathophysiology has not been ruled out. To gain a better understanding of the involvement of skeletal muscle pathophysiology in SMA, we have developed three aims: to identify cell-specific Smn-interacting proteins, to characterize postnatal skeletal muscle development in mouse models of SMA, and to assess the functional capacity of muscles from SMA model mice. We have used tandem affinity purification to discover Smn interacting partners in disease relevant cell types. We have identified novel cell-specific Smn interacting proteins of which we have validated myosin regulatory light chain as a muscle-specific Smn associated protein in vivo. We have taken advantage of two different mouse models of SMA, the severe Smn-/-;SMN2 mouse and the less severe Smn2B/- mouse, to study the postnatal development of skeletal muscle. Primary myoblasts from Smn2B/- mice demonstrate delayed myotube fusion and aberrant expression of the myogenic program. In addition, the expression of myogenic proteins was delayed in muscles from severe Smn-/-;SMN2 and less severe Smn2B/- SMA model mice. Muscle denervation and degeneration, however, are not the cause of the aberrant myogenic program. At the functional level, we demonstrate a significant decrease in force production in pre-symptomatic Smn-/-;SMN2 and Smn2B/- mice indicating that muscle weakness is an early event in these mice. Immunoblot analyses from hindlimb skeletal muscle samples revealed aberrant levels of developmentally regulated proteins important for muscle function, which may impact muscle force production in skeletal muscle of SMA model mice. The present study demonstrates early and profound intrinsic muscle weakness and aberrant expression of muscle proteins in mouse models of SMA, thus demonstrating how muscle defects can contribute to the disease phenotype independently of and in addition to that caused by motor neuron pathology.
Additional file 8 of A facile and scalable in production non-viral gene engineered mesenchymal stem cells for effective suppression of temozolomide-resistant (TMZR) glioblastoma growth
Creators: name: Tu, Geraldine Xue En
name: Ho, Yoon Khei
name: Ng, Zhi Xu
name: Teo, Ke Jia
name: Yeo, Tseng Tsai
name: Heng-Phon Too
Publisher: name: figshare
Year: 2020
Subjects: subject: Biophysics
subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Molecular Biology
subject: Pharmacology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Cancer
subject: Hematology
subject: Plant Biology
subject: 60506 Virology

description: Additional file 8. Scaling up MSC modification process on microcarrier culture. AD-MSCs were seeded at 28,000/cm2 on Cytodex® 3 microcarriers (total surface area of 1.9 cm2) in 24-well non-adherent plates, with agitation speed of 50 rpm for 24 h before transfection. Similar to flat-bed transfection, the polymer and DNA complex (at 200-300 ng of pDNA) were added to the cell culture using a dropwise manner after 15 min incubation. Cells were transfected in the presence or absence of the enhancer. Two-day post transfection, a the fluorescent images were captured at 10x magnification. Representative images are shown. b After which, cells were harvested through trypsinization. To separate the cells from microcarrier, the suspension cells were filtered through 70 μm cell strainer. The transfection efficiencies were determined through flow cytometry analysis. Cell viability based on PI exclusion was determined with NC-3000 automated cell counter. The combination chart presents % of GFP+ cells (graph bar) and cell viability (mark type) Data of biological triplicates are expressed as mean + SD. c For microcarriers with total surface area of 1.9 cm2 and 9.5 cm2, cells were transfected in 24-well non-adherent plates. To further scale up the transfection process, AD-MSCs were seeded on Cytodex® 3 (total surface area of 47.5 cm2) at 28,000 cells/cm2 in 125 mL Erlenmeyer flasks. One day later, cells were transfected in the presence of Enhancer. The amount of DNA was fixed at 150 ng/cm2 of microcarriers. Two-day post transfection, transfection efficiency was analysed. The scatter plot represents absolute number of GFP+ cells collected from each culture vessel; deduced from the readout of cell counting and flow cytometry analysis of the GFP expression. Data are expressed as mean + SD, n = 3.
Data Collection, Analysis, and Classification for the Development of a Sailing Performance Evaluation System
Creators: name: Sammon, Ryan
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2013
Subjects: artificial neural network
nearest-neighbor search
classification
inertial measurement unit
Kalman filtering
sailing

description: The work described in this thesis contributes to the development of a system to evaluate sailing performance. This work was motivated by the lack of tools available to evaluate sailing performance. The goal of the work presented is to detect and classify the turns of a sailing yacht. Data was collected using a BlackBerry PlayBook affixed to a J/24 sailing yacht. This data was manually annotated with three types of turn: tack, gybe, and mark rounding. This manually annotated data was used to train classification methods. Classification methods tested were multi-layer perceptrons (MLPs) of two sizes in various committees and nearest- neighbour search. Pre-processing algorithms tested were Kalman filtering, categorization using quantiles, and residual normalization. The best solution was found to be an averaged answer committee of small MLPs, with Kalman filtering and residual normalization performed on the input as pre-processing.