title: ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ ÑÐ¾Ð»Ð¸ ÑÐ¸Ð³Ð¼Ð°-1 ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° Ð² ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¸Ñ ÐºÐ¾Ð½ÑÐ°ÐºÑÐ¾Ð² Ð½ÐµÐ¹ÑÐ¾Ð½Ð¾Ð² ÑÑÑÐ¸Ð°ÑÑÐ¼Ð° Ð½Ð° Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ Ð¥Ð°Ð½ÑÐ¸Ð½Ð³ÑÐ¾Ð½Ð°
title: Ð¼Ð°Ð³Ð¸ÑÑÐµÑÑÐºÐ°Ñ Ð´Ð¸ÑÑÐµÑÑÐ°ÑÐ¸Ñ
titleType: Subtitle
Creators: name: ÐÐ°Ð¹Ð½ÑÐ»Ð»Ð¸Ð½Ð°, ÐÐ½Ð°ÑÑÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2016
Subjects: ÐÐµÐ¹ÑÐ¾Ð½Ñ
ÐÐ»ÐµÑÐºÐ° (Ð±Ð¸Ð¾Ð»)
Ð±Ð¾Ð»ÐµÐ·Ð½Ñ Ð¥Ð°Ð½ÑÐ¸Ð½Ð³ÑÐ¾Ð½Ð°
ÐºÐ»ÐµÑÐ¾ÑÐ½Ð°Ñ Ð¼Ð¾Ð´ÐµÐ»Ñ in vitro
ÐºÐ¾ÑÑÐ¸ÐºÐ¾-ÑÑÑÐ¸Ð°ÑÐ°Ð»ÑÐ½ÑÐµ ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¸Ðµ ÐºÐ¾Ð½ÑÐ°ÐºÑÑ
Huntington's disease
cell model in vitro
cortico-striatal synaptic contacts

description: ÐÐ¾ÑÑÐ¸ÐºÐ¾-ÑÑÑÐ¸Ð°ÑÐ°Ð»ÑÐ½ÑÐµ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐµ ÐºÑÐ»ÑÑÑÑÑ Ð±ÑÐ»Ð¸ Ð¾Ð¿ÑÐ¸Ð¼Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°Ð½Ñ Ñ ÑÐµÐ»ÑÑ ÑÐ¼Ð¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°ÑÑ Ð±Ð¾Ð»ÐµÐ·Ð½Ñ Ð¥Ð°Ð½ÑÐ¸Ð½Ð³ÑÐ¾Ð½Ð° in vitro. ÐÐµÑÐ¾Ð´Ð¾Ð¼ Ð¸Ð¼Ð¼ÑÐ½Ð¾ÑÐ¸ÑÐ¾ÑÐ¸Ð¼Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¾ÐºÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ñ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð° ÑÐ»Ð¸Ð¼Ð¸Ð½Ð°ÑÐ¸Ñ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð² Ð½ÐµÐ¹ÑÐ¾Ð½Ð¾Ð² ÑÑÑÐ¸Ð°ÑÑÐ¼Ð° Ð½Ð° 21 Ð´ÐµÐ½Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð² ÐºÑÐ»ÑÑÑÑÐ°Ñ Ð¼ÑÑÐ°Ð½ÑÐ½Ð¾Ð³Ð¾ ÑÐ¸Ð¿Ð° YAC128, Ð½Ðµ Ð½Ð°Ð±Ð»ÑÐ´Ð°ÐµÐ¼Ð°Ñ Ð² ÐºÑÐ»ÑÑÑÑÐ°Ñ Ð´Ð¸ÐºÐ¾Ð³Ð¾ ÑÐ¸Ð¿Ð° (WT). Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²ÐµÐ½Ð½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð· ÑÐµÐ½Ð¾ÑÐ¸Ð¿Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¿ÑÐ¾ÑÐ²Ð»ÐµÐ½Ð¸Ñ Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ Ð¿ÑÑÑÐ¼ Ð¿Ð¾Ð´ÑÑÑÑÐ° Ð¸ Ð¾ÑÐµÐ½ÐºÐ¸ ÑÐ¾ÑÐ¼Ñ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð² Ð½Ð° Ð¿Ð¾ÑÑÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ ÑÑÐ¾ÑÐ¾Ð½Ðµ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÐºÐ¾Ð¼Ð¿ÑÑÑÐµÑÐ½Ð¾Ð³Ð¾ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ð½Ð¾Ð³Ð¾ Ð¾Ð±ÐµÑÐ¿ÐµÑÐµÐ½Ð¸Ñ Neuron Studio. ÐÑÐ¾Ð´ÐµÐ¼Ð¾Ð½ÑÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¾ ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ðµ Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÐ¸ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð² Ð² ÐºÑÐ»ÑÑÑÑÐ°Ñ Ð¼ÑÑÐ°Ð½ÑÐ½Ð¾Ð³Ð¾ ÑÐ¸Ð¿Ð° Ð¿ÑÐ¸ Ð´Ð¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸Ð¸ Ð² ÐºÑÐ»ÑÑÑÑÐ°Ð»ÑÐ½ÑÑ ÑÑÐµÐ´Ñ Ð°Ð³Ð¾Ð½Ð¸ÑÑÐ° ÑÐ¸Ð³Ð¼Ð°-1 ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° 3-Ð Ð Ð . ÐÐ¾ÐºÐ´Ð°ÑÐ½ ÑÐ¸Ð³Ð¼Ð°-1 ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° Ð²ÑÐ·Ð²Ð°Ð» ÑÐ»Ð¸Ð¼Ð¸Ð½Ð°ÑÐ¸Ñ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð² Ð² ÐºÑÐ»ÑÑÑÑÐ°Ñ Ð´Ð¸ÐºÐ¾Ð³Ð¾ ÑÐ¸Ð¿Ð° Ð¸ Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐµÐµ ÑÐ¼ÐµÐ½ÑÑÐµÐ½Ð¸Ðµ Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÐ¸ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð² Ð² YAC128 ÐºÑÐ»ÑÑÑÑÐ°Ñ. ÐÑÐ¸ ÑÑÐ¾Ð¼ Ð¾ÑÐ¼ÐµÑÐµÐ½Ð¾ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ ÑÐ¾Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ñ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ Ð²Ð¸Ð´Ð¾Ð² ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð². ÐÑÑÑÑÑÑÐ²Ð¸Ðµ ÑÑÑÐµÐºÑÐ° Ð²Ð¾ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¸Ñ Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÐ¸ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð² Ð¿Ð¾ÑÐ»Ðµ Ð´Ð¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸Ñ 3-Ð Ð Ð  Ð² ÐºÑÐ»ÑÑÑÑÐ°Ð»ÑÐ½ÑÑ ÑÑÐµÐ´Ñ Ð½ÐµÐ¹ÑÐ¾Ð½Ð¾Ð² Ñ Ð½Ð¾ÐºÐ´Ð°ÑÐ½Ð¾Ð¼ ÑÐ¸Ð³Ð¼Ð°-1 ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° Ð´Ð¾ÐºÐ°Ð·Ð°Ð»Ð¾ ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐ½Ð¾ÑÑÑ ÐµÐ³Ð¾ Ð²Ð¾Ð·Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ð¸Ð¼ÐµÐ½Ð½Ð¾ Ð½Ð° Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼ÑÐ¹ ÑÐµÑÐµÐ¿ÑÐ¾Ñ. Ð¡ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¾ÑÐ¼ÑÐ²ÐºÐ¸ Ð°ÐºÑÐ¸Ð²Ð¸ÑÑÑÑÐµÐ³Ð¾ Ð°Ð³ÐµÐ½ÑÐ° Ñ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐµÐ¹ ÑÐ¸ÐºÑÐ°ÑÐ¸ÐµÐ¹ ÐºÐ»ÐµÑÐ¾Ðº Ð¸ Ð¿Ð¾Ð´ÑÑÑÑÐ¾Ð¼ Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÐ¸ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð² Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð° Ð´Ð»Ð¸ÑÐµÐ»ÑÐ½Ð¾ÑÑÑ ÑÑÑÐµÐºÑÐ° Ð°ÐºÑÐ¸Ð²Ð°ÑÐ¸Ð¸ ÑÐ¸Ð³Ð¼Ð°-1 ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ°, Ð¾ÐºÐ°Ð·Ð°Ð²ÑÐ°ÑÑÑ ÑÐ°Ð²Ð½Ð¾Ð¹ Ð¿ÑÐ¸Ð¼ÐµÑÐ½Ð¾ 8 ÑÐ°ÑÐ°Ð¼ Ð² ÑÐ°Ð¼ÐºÐ°Ñ Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼Ð¾Ð¹ Ð¼Ð¾Ð´ÐµÐ»Ð¸. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ ÑÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÐ¸ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐ°Ð½Ñ Ð¸ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÐµÐ½Ñ Ð² Ð²Ð¸Ð´Ðµ Ð³ÑÐ°ÑÐ¸ÐºÐ¾Ð² Ð¸ ÑÑÐ¾Ð»Ð±Ð¸ÐºÐ¾Ð²ÑÑ Ð´Ð¸Ð°Ð³ÑÐ°Ð¼Ð¼.
description: Cortico-striatal cell culture was optimized to model Huntington's disease in vitro. On the 21st day of in vitro cultivation spine elimination of striatal neurons was shown by the method of immunocytochemistry in mutant cultures (YAC128) but not in wild type (WT) ones. Quantitative analysis was worked out to evaluate phenotypic expression of the disease. Spine counting and its shape analysis were performed automatically by Neuron Studio program. Spine rescue in YAC128 cultures was shown after sigma-1 receptor's agonist 3-PPP exposure. Knockdown of sigma-1 receptor caused spine elimination both in YAC128 and WT cultures. Herein change in the ratio of different spine types was marked. No spine rescue effect after 3-PPP exposure to knockdown cell cultures evidenced for its specific action on sigma-1 receptor. Washing 3-PPP out and cell fixing with further spine counting made it possible to estimate time of spine rescue effect which occurred to be 8 hours approximately within the framework of the working model. The results were statistically processed and presented as graphs and bar charts.