title: ÐÐ¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð¾ÐºÐ¸ÑÐ»Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð¹ Ð±Ð¸Ð¾ÑÑÐ°Ð½ÑÑÐ¾ÑÐ¼Ð°ÑÐ¸Ð¸ Ð´Ð¸ÐºÐ»Ð¾ÑÐµÐ½Ð°ÐºÐ° in vitro Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð¹ TIO2 ÐÐÐÐÐ Ð¼Ð¸ÑÐµÐ½Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Simulation of oxidative biotransformation of diclofenac in vitro using a modified TiO2 MALDI target
Creators: name: Ð¡ÐµÐ²Ð½Ð¸ÑÐºÐ°Ñ, ÐÐ»Ð¸Ð·Ð°Ð²ÐµÑÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: Ð´Ð¸Ð¾ÐºÑÐ¸Ð´ ÑÐ¸ÑÐ°Ð½Ð°
ÑÐ¾ÑÐ¾ÐºÐ°ÑÐ°Ð»Ð¸Ð·
ÑÐ¾ÑÐ¾ÐºÐ°ÑÐ°Ð»Ð¸ÑÐ¸ÑÐµÑÐºÐ¾Ðµ Ð¾ÐºÐ¸ÑÐ»ÐµÐ½Ð¸Ðµ
ÑÐ»ÐµÐºÑÑÐ¾Ñ Ð¸Ð¼Ð¸ÑÐµÑÐºÐ¾Ðµ Ð¾ÐºÐ¸ÑÐ»ÐµÐ½Ð¸Ðµ
Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¼ Ð»ÐµÐºÐ°ÑÑÑÐ²ÐµÐ½Ð½ÑÑ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð²
ÐÐÐÐÐ ÐÐ¡
titanium dioxide
photocatalysis
photocatalysis oxidation
electrochemical oxidation
drug metabolism
MALDI MS

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¸Ð¼Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¸ ÑÐ¾ÑÐ¾ÐºÐ°ÑÐ°Ð»Ð¸ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¾ÐºÐ¸ÑÐ»ÐµÐ½Ð¸Ñ Ð´Ð¸ÐºÐ»Ð¾ÑÐµÐ½Ð°ÐºÐ°. ÐÐ° Ð¾ÑÐ½Ð¾Ð²Ð°Ð½Ð¸Ð¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð½ÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¿ÑÐµÐ´Ð»Ð¾Ð¶ÐµÐ½ Ð¿Ð¾Ð´ÑÐ¾Ð´, Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÑÑÐ¸Ð¹ Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸ÑÑ Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ ÑÐ¾ÑÐ¾ÐºÐ°ÑÐ°Ð»Ð¸ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¾ÐºÐ¸ÑÐ»ÐµÐ½Ð¸Ñ ÐºÑÐµÐ½Ð¾Ð±Ð¸Ð¾ÑÐ¸ÐºÐ¾Ð² Ð½ÐµÐ¿Ð¾ÑÑÐµÐ´ÑÑÐ²ÐµÐ½Ð½Ð¾ Ð½Ð° ÐÐÐÐÐ Ð¼Ð¸ÑÐµÐ½Ð¸ Ñ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐ¸Ð¼ Ð¼Ð°ÑÑ-ÑÐ¿ÐµÐºÑÑÐ¾Ð¼ÐµÑÑÐ¸ÑÐµÑÐºÐ¸Ð¼ Ð°Ð½Ð°Ð»Ð¸Ð·Ð¾Ð¼. ÐÐ° Ð¾ÑÐ½Ð¾Ð²Ð°Ð½Ð¸Ð¸ ÑÑÐ°Ð²Ð½Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð¿ÑÐ¾Ð´ÑÐºÑÐ¾Ð² Ð¾ÐºÐ¸ÑÐ»ÐµÐ½Ð¸Ñ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÑÐ°Ð½Ð´ÐµÐ¼Ð½Ð¾Ð¹ Ð¼Ð°ÑÑ-ÑÐ¿ÐµÐºÑÑÐ¾Ð¼ÐµÑÑÐ¸Ð¸ Ð²ÑÑÐ¾ÐºÐ¾Ð³Ð¾ ÑÐ°Ð·ÑÐµÑÐµÐ½Ð¸Ñ Ð±ÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð£Ð¤-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ðµ ÑÐ¾ÑÐ¾ÐºÐ°ÑÐ°Ð»Ð¸ÑÐ¸ÑÐµÑÐºÐ¾Ðµ Ð¾ÐºÐ¸ÑÐ»ÐµÐ½Ð¸Ðµ Ð² Ð¿ÑÐ¸ÑÑÑÑÑÐ²Ð¸Ðµ Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸Ñ TiO2(Ð£Ð¤/TiO2-Ð¤ÐÐ) ÑÐ²Ð»ÑÐµÑÑÑ Ð±Ð¾Ð»ÐµÐµ Ð¿ÑÐµÐ´Ð¿Ð¾ÑÑÐ¸ÑÐµÐ»ÑÐ½ÑÐ¼ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð¼Ð¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¾ÐºÐ¸ÑÐ»Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¼Ð° Ð´Ð¸ÐºÐ»Ð¾ÑÐµÐ½Ð°ÐºÐ°, ÑÐµÐ¼ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¸Ð¼Ð¸ÑÐµÑÐºÐ¾Ðµ Ð¾ÐºÐ¸ÑÐ»ÐµÐ½Ð¸Ðµ. ÐÑÐµÐ´Ð»Ð¾Ð¶ÐµÐ½ Ð¿ÑÐ¾ÑÑÐ¾Ð¹, Ð±ÑÑÑÑÑÐ¹ Ð¸ Ð²ÑÑÐ¾ÐºÐ¾Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´Ð¸ÑÐµÐ»ÑÐ½ÑÐ¹ Ð¿Ð¾Ð´ÑÐ¾Ð´ Ð´Ð»Ñ Ð¼Ð¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¾ÐºÐ¸ÑÐ»Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¼Ð°, Ð¾ÑÐ½Ð¾Ð²Ð°Ð½Ð½ÑÐ¹ Ð½Ð° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ð¸ Ð£Ð¤/ TiO2 -Ð¤ÐÐ Ð½ÐµÐ¿Ð¾ÑÑÐµÐ´ÑÑÐ²ÐµÐ½Ð½Ð¾ Ð½Ð° ÐÐÐÐÐ Ð¼Ð¸ÑÐµÐ½Ð¸ Ñ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐ¸Ð¼ Ð¼Ð°ÑÑ-ÑÐ¿ÐµÐºÑÑÐ¾Ð¼ÐµÑÑÐ¸ÑÐµÑÐºÐ¸Ð¼ Ð°Ð½Ð°Ð»Ð¸Ð·Ð¾Ð¼. ÐÐ°ÑÑ-ÑÐ¿ÐµÐºÑÑÑ Ð¸ Ð²ÑÑÐ¾Ð´ Ð¿ÑÐ¾Ð´ÑÐºÑÐ¾Ð² Ð¾ÐºÐ¸ÑÐ»ÐµÐ½Ð¸Ñ Ð´Ð¸ÐºÐ»Ð¾ÑÐµÐ½Ð°ÐºÐ°, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð¿ÑÑÑÐ¼ ÑÑÐ°Ð½Ð´Ð°ÑÑÐ½Ð¾Ð³Ð¾ Ð£Ð¤/ TiO2-Ð¤ÐÐ Ð² Ð¾Ð±ÑÐµÐ¼Ðµ Ð¸ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¿ÑÐµÐ´Ð»Ð¾Ð¶ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð²Ð°ÑÐ¸Ð°Ð½ÑÐ° Ð½Ð° ÐÐÐÐÐ Ð¼Ð¸ÑÐµÐ½Ð¸ Ð¿ÑÐµÐºÑÐ°ÑÐ½Ð¾ ÑÐ¾Ð³Ð»Ð°ÑÑÑÑÑÑ Ð¼ÐµÐ¶Ð´Ñ ÑÐ¾Ð±Ð¾Ð¹
description: This work is devoted to the study of electrochemical and photocatalytic oxidation of diclofenac. Based on the studies, an approach was proposed that allows the study of the photocatalytic oxidation of xenobiotics in outer space. Based on a comparative analysis of oxidation products using high resolution tandem mass spectrometry, it was shown that UV-induced photocatalytic oxidation in the presence of TiO2 nanoparticles (UV /TiO2-FCO) is a more preferable method for modeling the oxidative metabolism of diclofenac than electrochemical oxidation. A simple, fast, and high-performance approach is proposed for modeling oxidative metabolism, based on UV / TiO2-FCO directly on the MALDI target, followed by mass spectrometric analysis. Mass spectra and yield of diclofenac oxidation products obtained by standard UV / TiO2-FCO in volume and using the proposed option on MALDI targets are in excellent agreement