cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
A genetic tool kit for cellular and behavioral analyses of insect sugar receptors
Creators: name: Yavuz, Ahmet
name: Jagge, Christopher
name: Slone, Jesse
name: Amrein, Hubert
Publisher: name: Taylor & Francis
Year: 2014
Subjects: subject: Developmental Biology
subject: Biological Sciences
subject: Ecology
subject: FOS: Biological sciences
subject: Evolutionary Biology
subject: Molecular Biology
subject: Genetics
subject: Microbiology

description: Arthropods employ a large family of up to 100 putative taste or gustatory receptors (Grs) for the recognition of a wide range of non-volatile chemicals. In Drosophila melanogaster, a small subfamily of 8 Gr genes is thought to mediate the detection of sugars, the fly's major nutritional source. However, the specific roles for most sugar Gr genes are not known. Here, we report the generation of a series of mutant sugar Gr knock-in alleles and several composite sugar Gr mutant strains, including a sugar blind strain, which will facilitate the characterization of this gene family. Using Ca2+ imaging experiments, we show that most gustatory receptor neurons (GRNs) of sugar blind flies (lacking all 8 sugar Gr genes) fail to respond to any sugar tested. Moreover, expression of single sugar Gr genes in most sweet GRNs of sugar-blind flies does not restore sugar responses. However, when pair-wise combinations of sugar Gr genes are introduced to sweet GRNs, responses to select sugars are restored. We also examined the cellular phenotype of flies homozygous mutant for Gr64a, a Gr gene previously reported to be a major contributor for the detection of many sugars. In contrast to these claims, we find that sweet GRNs of Gr64a homozygous mutant flies show normal responses to most sugars, and only modestly reduced responses to maltose and maltotriose. Thus, the precisely engineered genetic mutations of single Gr genes and construction of a sugar-blind strain provide powerful analytical tools for examining the roles of Drosophila and other insect sugar Gr genes in sweet taste.
MPEC 2024-V11 : 2024 UP11
Creators: name: Minor Planet Center Staff
Publisher: name: The Minor Planet Center
Year: 2024
Subjects: subject: Small Solar System bodies
valueUri: http://astrothesaurus.org/uat/1469
subject: 2024 UP11

description: The Minor Planet Electronic Circulars contain information on unusual minor planets, routine data on comets and natural satellites, and occasional editorial announcements.  They are published on behalf of Division F of the International Astronomical Union by the Minor Planet Center, Smithsonian Astrophysical Observatory, Cambridge, MA 02138, U.S.A.
Characterization of Particulate Matter Morphology and Volatility from a Compression-Ignition Natural Gas Direct-Injection Engine
Creators: name: Graves, Brian
name: Olfert, Jason
name: Patychuk, Bronson
name: Dastanpour, Ramin
name: Rogak, Steven
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Ecology
subject: FOS: Biological sciences
subject: Earth and Environmental Sciences
subject: Biotechnology
subject: Physics
subject: Engineering
subject: Biochemistry
subject: Biophysics

description: The particulate matter emitted from a single-cylinder compression-ignition, natural-gas engine fitted with a High-Pressure Direct-Injection (HPDI) system distinctly different from a duel fuel engine was investigated, and characterized by size distribution, morphology, mass-mobility exponent, effective density, volatility, mixing state, and primary particle size using transmission electron microscopy (TEM), and tandem measurements from differential mobility analyzers (DMA) and a centrifugal particle mass analyser (CPMA). Six engine conditions were selected which varied load, speed, EGR fraction, and fuel delivery strategy. An increase in engine load increased both the number concentration and the geometric mean diameter of the particulate. The fraction of the number of purely volatile particles to total number of particles (number volatile fraction, NVF) was found to decrease as load increased, although the lower speed, partially premixed mode had the lowest NVF. All size distributions were also found to be unimodal. The size-segregated ratio of the mass of internally mixed volatile material to total particle mass (MVF) decreased with load and with particle mobility-equivalent diameter. A roughly constant amount of volatile material is likely produced at each engine mode, and the decrease in MVF is due to the increase in PM number with load. Effective density and mass-mobility exponent of the non-volatile soot at the different engine loads was the same or slightly higher than soot from traditional Diesel engines. Denuded effective density trends were observed to collapse to approximately the same line, although engine modes with higher MVFs had slightly higher effective densities suggesting that the soot structures have collapsed into more dense shapes: a suspicion that is confirmed with TEM images. TEM results also indicated that primary particle size first decreases from low to medium load, then increases from medium to high load. An increase in EGR was also seen to increase primary particle size. Coefficients were determined for a relation which gives primary particle diameter as a function of projected area equivalent diameter. A decrease in load or speed results in a stronger correlation.
Lessons from the street: leading complex work at the front line
Creators: name: Cherry, Nita
Publisher: name: Swinburne
Year: 2024

description: Emerging academic commentary asks how people can be prepared for work and leadership under conditions of increasing complexity. It has been noted, however, that treatments of leadership in the management literature have been slow to engage with complexity thinking and also remain largely focused on executive and middle level leadership. This paper explores the implications of complexity thinking for front-line leadership work. It does that, firstly, by considering leadership questions implied by newer theoretical perspectives on complex practice and secondly, by revisiting earlier insights into complex work at street level. Lipsky’s (1980) exploration of street-level bureaucracy suggested that unresolvable paradoxes in public policy have always created significant dilemmas for front-line practitioners and leaders. Policing, education and welfare are prime examples of this space. Indeed, Perez (2011) suggests that operational police and their leaders face uniquely complex dilemmas. Thirdly, then, this paper references research undertaken with 50 serving police officers in Melbourne to focus on key issues for contemporary front-line leadership.
Towards the development of a novel proteomic tool to map trimethyllysine marks on histones via a Hofmann-type chemical modification.
Creators: name: Ekinci, Melis Eda
Publisher: name: Imperial College London
Year: 2019

description: One of the key epigenetic processes for transcriptional control is dynamic post-translational modification (PTM) of histone proteins. Dysregulation of these epigenetic mechanisms have been linked to the aetiology of human diseases such as cancer and neurological disorders. To decipher the epigenetic pathways leading to the development of disease and to gain insight into the function of post-translational modifications of histones, a great interest was taken on the genome-wide mapping of epigenetic marks. The most commonly used method to profile PTMs on histones is the ChIP-seq technique (chromatin immunoprecipitation followed by DNA sequencing), which is based on the use of antibodies to target and enrich specific epigenetic marks. However, the dependence of ChIP-seq on antibodies constitutes a significant limitation due to possible cross-reactivity and poor specificity of those antibodies towards the targeted epigenetic marks. In addition, the ChIP-seq technique only allows the mapping of single epigenetic marks to genomic loci. Here we present a novel antibody-free chemical biological tool ‘CLICK-seq’ to map combinatorial histone lysine trimethylation marks on intact mono-nucleosomes. It is based on the selective modification of trimethyllysine residues on histone proteins to obtain an alkene functionality on these substrates, which enables the introduction of affinity tags via a thiol-ene click chemistry for selective enrichment. This work demonstrates diverse chemical approaches taken towards the elimination of trimethylamine in quaternary ammonium substrates to achieve the formation of an alkene under mild and ‘protein-compatible’ conditions. With the development of a novel reaction - Pd-catalysed Hofmann-type elimination – the removal of trimethylamine was achieved at low temperature on model small molecules (albeit in a low yield). In the second project, a novel design for acid-cleavable cross-linkers was developed to simultaneously achieve the cross-linking of histone protein complexes and the subsequent chemical labelling of histone lysine residues upon acid-catalysed cleavage. This work portrays our efforts towards the synthesis of a small library of acid-cleavable cross-linkers.
Comparative analysis of betalain content in Stenocereus stellatus fruits and other cactus fruits using principal component analysis
Creators: name: Pérez-Loredo, María Guadalupe
name: García-Ochoa, Felipe
name: Barragán-Huerta, Blanca E.
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Plant Biology
subject: FOS: Biological sciences
subject: Science Policy
subject: 60506 Virology
subject: Biological Sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: Ecology
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Cell Biology
subject: Microbiology
subject: Medicine

description: Stenocereus stellatus fruits from Puebla, Mexico, with red, purple, yellow, and white pulp were characterized regarding their total betalain (TB) content, and physicochemical properties. The TB content was 856.07–2968 µg per gram of pulp dry weight (DW). Using principal component analysis, the levels of pigments in S. stellatus fruits were compared with 32 samples from other cactus fruits. As a result, 3 confidence intervals for TB content (mg g−1) were calculated: low (< 1208), medium (2935–3288) and high (4488–9248). Thus, the cactus fruits can be categorized as a poor, good, and excellent source of betalain pigments.
Leadership in liquid modernity
Creators: name: Lippi, Julian
Publisher: name: Swinburne
Year: 2024

description: This paper explores possibilities for refreshing thinking about leadership from perspectives of liquid modernity suggested by Bauman (2000), who identified several dimensions of modern uncertainty in human life and work. Reflecting unease with post-modernism, Bauman’s work has been influential in contemporary understandings of society, culture, learning and identity. It is also being taken up in emerging explorations of liquid learning in the educational literature. However, its implications for leadership remain largely unexplored in the management literature. This paper considers how current conceptions of leadership that emphasise the power of individual agency might be usefully re-considered in the light of Bauman’s contribution and it develops a number of generative questions to help that process. It then turns to conceptions of liquid learning to suggest some navigational aids for leading under conditions of liquidity.
A Genetic Tool Kit for Cellular and Behavioral Analyses of Insect Sugar Receptors
Creators: name: Yavuz, Ahmet
name: Jagge, Christopher
name: Slone, Jesse
name: Amrein, Hubert
Publisher: name: Taylor & Francis
Year: 2014
Subjects: subject: Developmental Biology
subject: Biological Sciences
subject: Ecology
subject: FOS: Biological sciences
subject: Evolutionary Biology
subject: Molecular Biology
subject: Genetics
subject: Microbiology

description: SUMMARYArthropods employ a large family of up to 100 putative taste or gustatory receptors (Grs) for the recognition of a wide range of non-volatile chemicals. In Drosophila melanogaster, a small subfamily of eight Gr genes is thought to mediate the detection of sugars, fly's major nutritional source. However, the specific roles for most sugar Gr genes are not known. Here, we report the generation of a series of mutant sugar Gr knock-in alleles and several composite sugar Gr mutant strains, including a sugar blind strain, which will facilitate the characterization of this gene family. Using Ca2+ imaging experiments, we show that most gustatory receptor neurons (GRNs) of sugar blind flies (lacking all eight sugar Gr genes) fail to respond to any sugar tested. Moreover, expression of single sugar Gr genes in most sweet GRNs of sugar-blind flies does not restore sugar responses. However, when pair-wise combinations of sugar Gr genes are introduced to sweet GRNs, responses to select sugars are restored. We also examined the cellular phenotype of flies homozygous mutant for the Gr64a gene, a sugar receptor previously reported to be a major contributor for the detection of many sugars. In contrast to these claims, we find that sweet GRNs of Gr64a homozygous mutant flies show normal responses to most sugars, and only modestly reduced responses to maltose and maltotriose. Thus, the precisely engineered genetic mutations of single Gr genes and construction of a sugar-blind strain provide powerful analytical tools for examining the roles of Drosophila and other insect sugar Gr genes in sweet taste.
The mRMR variable selection method: a comparative study for functional data
Creators: name: J.R. Berrendero
name: A. Cuevas
name: J.L. Torrecilla
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Cancer
subject: Mathematics
subject: FOS: Mathematics
subject: Biological Sciences
subject: Chemistry
subject: Biotechnology
subject: Biochemistry

description: The use of variable selection methods is particularly appealing in statistical problems with functional data. The obvious general criterion for variable selection is to choose the ‘most representative’ or ‘most relevant’ variables. However, it is also clear that a purely relevance-oriented criterion could lead to select many redundant variables. The minimum Redundance Maximum Relevance (mRMR) procedure, proposed by Ding and Peng [Minimum redundancy feature selection from microarray gene expression data. J Bioinform Comput Biol. 2005;3:185–205] and Peng et al. [Feature selection based on mutual information: criteria of max-dependency, max-relevance, and min-redundancy. IEEE Trans Pattern Anal Mach Intell. 2005;27:1226–1238] is an algorithm to systematically perform variable selection, achieving a reasonable trade-off between relevance and redundancy. In its original form, this procedure is based on the use of the so-called mutual information criterion to assess relevance and redundancy. Keeping the focus on functional data problems, we propose here a modified version of the mRMR method, obtained by replacing the mutual information by the new association measure (called distance correlation) suggested by Székely et al. [Measuring and testing dependence by correlation of distances. Ann Statist. 2007;35:2769–2794]. We have also performed an extensive simulation study, including 1600 functional experiments (100 functional models ×4 sample sizes ×4 classifiers) and three real-data examples aimed at comparing the different versions of the mRMR methodology. The results are quite conclusive in favour of the new proposed alternative.
A genetic tool kit for cellular and behavioral analyses of insect sugar receptors
Creators: name: Yavuz, Ahmet
name: Jagge, Christopher
name: Slone, Jesse
name: Amrein, Hubert
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Developmental Biology
subject: Biological Sciences
subject: Ecology
subject: FOS: Biological sciences
subject: Evolutionary Biology
subject: Molecular Biology
subject: Genetics
subject: Microbiology

description: Arthropods employ a large family of up to 100 putative taste or gustatory receptors (Grs) for the recognition of a wide range of non-volatile chemicals. In Drosophila melanogaster, a small subfamily of 8 Gr genes is thought to mediate the detection of sugars, the fly's major nutritional source. However, the specific roles for most sugar Gr genes are not known. Here, we report the generation of a series of mutant sugar Gr knock-in alleles and several composite sugar Gr mutant strains, including a sugar blind strain, which will facilitate the characterization of this gene family. Using Ca2+ imaging experiments, we show that most gustatory receptor neurons (GRNs) of sugar blind flies (lacking all 8 sugar Gr genes) fail to respond to any sugar tested. Moreover, expression of single sugar Gr genes in most sweet GRNs of sugar-blind flies does not restore sugar responses. However, when pair-wise combinations of sugar Gr genes are introduced to sweet GRNs, responses to select sugars are restored. We also examined the cellular phenotype of flies homozygous mutant for Gr64a, a Gr gene previously reported to be a major contributor for the detection of many sugars. In contrast to these claims, we find that sweet GRNs of Gr64a homozygous mutant flies show normal responses to most sugars, and only modestly reduced responses to maltose and maltotriose. Thus, the precisely engineered genetic mutations of single Gr genes and construction of a sugar-blind strain provide powerful analytical tools for examining the roles of Drosophila and other insect sugar Gr genes in sweet taste.
Colonic Mass After NSAID Use and Concerns for Malignancy
Creators: name: Ho, Kimberly
name: Garcia-Aguilar, Julio
name: Nagao, Sayaka
name: Jacobson, Ira
Publisher: name: PRIME
Year: 2024
Subjects: classificationCode: 320209
subject: Gastroenterology and hepatology

description: No abstract available
High mobility organic semiconductors for microelectronic applications
Creators: name: Panidi, Ioulianna
Publisher: name: Imperial College London
Year: 2019

description: The potential of organic electronic devices, over the past few years, has been proven tremendous. Flexible and transparent electronic devices will soon enter the market, but there are still some challenging aspects requiring further improvements. A generic approach to enhance the charge transport properties of the organic semiconductors is by introducing molecular additives. In particular for thin lm transistors, doping the semiconductor layer leads to higher charge carrier mobility, as well as improving several parameters of the device. The work in this thesis focuses on identifying suitable molecular additives that can dope organic semiconductors for thin lm transistors. Chapter 5 presents the application of tris(penta uorophenyl)borane B(C6F5)3 as an e ective p-type dopant. B(C6F5)3 was applied in several small molecule:polymer blends and the improvement in the charge transport properties, as measured from organic thin lm transistors (OTFTs), was remarkable. In particular the blend diF-TESADT:PTAA and the C8-BTBT:C16-IDT-BT showed hole mobility of 8 and 11 cm2/Vs, respectively. OTFT parameters, such as the threshold voltage and the contact resistance were signi cantly reduced with the addition of molecular additives. The impact of the dopant addition in the blends was studied by AFM and XRD, which revealed that long-range crystallinity was induced in certain systems. Chapter 6 presents the DMBI-BDZC as a new n-type dopant. For transistors integration into circuits balanced charge transport is required and thus extensive optimisation is still required for n-type materials. DMBI-BDZC was used to dope a small molecule (NDI3HU-DTYM2) and a polymeric (N2200) semiconductor. In both systems free electron generation was observed at an optimum dopant concentration, as measured by electron paramagnetic resonance (EPR). OTFTs with enhanced charge carrier mobility and reduced contact resistance was achieved with the dopant addition. By further analysis of the device characteristics, reduction in both activation energy and density of traps states was observed in the doped devices. The morphology studies of the organic semiconducting thin lms revealed that the dopant addition is not disrupting the structure. Furthermore, the reduction in activation energy at low voltages is in accordance with the trap density calculations as performed from the OTFTs. In chapter 7 a single organic layer light emitting transistor (OLET) is demonstrated. The key behind this device structure was the identi cation of the polymer PDITTT. This polymer combines high charge transport properties (1 cm2/Vs) as well as having green light emission. The properties of the polymer allowed the fabrication of a single layer organic semiconducting OLET. Blending the polymer with the small molecule C8-BTBT resulted in enhanced charge transport OTFTs. OTFTs and OLETs were fabricated from both the PDITTT and C8-BTBT:PDITTT.
A longitudinal study of student outcomes from participation in an international study tour: some preliminary findings
Creators: name: Tucker, Mark
name: Weaver-Cowdroy, Debbi
Publisher: name: Swinburne
Year: 2024

description: Students returning from an international business study tour program were interviewed about their experiences and perceptions of the professional and personal impact of the program. When interviews were conducted within 3-4 months of the students' return, mixed responses were received, with some students highly positive about their experiences, but other students highly distressed about the level of independence required of them. When students were consulted two to six years after the completion of the program, including re-interviewing the initial study participants, students appeared more able to appreciate the benefits of the program. This was evident in their responses that highlighted an improvement in discipline-based knowledge, a better understanding of personal and intercultural issues, and the further development of their generic academic skills. Negative emotions had all but disappeared from students' responses. While these findings are from small sample sizes and are not conclusive, the results raise important questions about the timing of critical program evaluations.
Cool beats and timely accents
Creators: name: Tofts, Darren
name: Gye, Lisa
Publisher: name: Swinburne
Year: 2024

description: In 1978 the music critic Lester Bangs published a typically pugnacious essay with the fighting title, 'The Ten Most Ridiculous Albums of the Seventies'. Before deliciously launching into his execution of Uri Geller's self-titled album or Rick Dees' The Original Disco Duck, Bangs asserts that because that decade was history's silliest, it stands to reason 'that ridiculous records should become the norm instead of anomalies', that abominations should be the best of our time. This absurd pretzel logic sounds uncannily like Jacques Derrida's definition of the 'post condition, since for it to arrive it begins by not arriving. Lester is thinking like a poststructuralist. The oddness of the most singularly odd album out in Bangs' greatest misses of the seventies had nothing to do with how ridiculous it was, but the fact that it even existed at all.
MORPHEME ANALYSIS OF ENGLISH LANGUAGE
Creators: name: Ma'murova, Diyora
name: Astanova, Dilafruz
Publisher: name: Zenodo
Year: 2024

description: Morphology  is  the study of  words. Morphemes are the minimal units  of  words  that have  a  meaning  and  can  not  be  subdivided  further.  There  are two  main types:  free  and bound.  Morpheme  is  article  includes  a  listof  references,  but  its  sources  remain  unclear because  it  has  insufficient.Therefore  the  researchers  conducted  the  research  about morpheme. The research  methode  that  was  used  is  decriptive  qualitative  method. To gain the  data  the researchers  used  literature  review in which  the data gained  from  dictionary.Every  morpheme  can  be  classified  as  either  free  or  bound.  Bound  morphemes  can  be further classified as derivational or inflectional. These categories are mutually exclusive, and as such, a given morpheme will belong to  exactly  one  of  them.  Free  morphemes  can  function  independently  as  words.  Bound morphemes  appear  only  as  part  us  of  words,  always  in  conjunction  with  a  root  and sometimes with other bound morphemes.
Effects of the Endobarrier on eating behaviour in humans
Creators: name: Aldhwayan, Madhawi
Publisher: name: Imperial College London
Year: 2019

description: Background: Roux-en-Y gastric bypass (RYGB) surgery decreases hunger, increases satiety and changes food preferences. It is unclear which component of the RYGB is responsible for these changes. The duodenal jejunal bypass liner (DJBL) is used for the treatment of patients with obesity and type 2 diabetes and reproduces the exclusion of the proximal small intestine of the RYGB. The objective of this study was to employ a reductionist approach to determine whether the bypass of the proximal intestine is the component conferring the effects of RYGB on eating behaviour using the DJBL as a research tool. Aims: To investigate the effects of the DJBL versus standard medical care for patients with obesity and T2DM on: a) Total caloric intake and food preferences b) Sweet taste detection and intensity. c) Appetitive and consummatory reward value of sweet taste. e) Psychological aspects of eating behaviour. f) Fasting and postprandial concentrations of glucose, insulin, GLP-1, PYY. Methods: This thesis was part of a large randomised clinical trial comparing standard medical therapy with vs. without the DJBL for patients with obesity and type 2 diabetes. Forty-seven participants were involved in this nested study (27 DJBL group and 20 control group). Mechanistic visits were performed at baseline, ten days, six months, 12 months, and 24 months after intervention using direct and indirect assessments. Results: 1. Both groups significantly reduced total caloric intake up to 12 months, then gradually started to increase through the second year. 2. There were no consistent changes in food preferences. 3. There were no significant changes within or between groups in the sensory or reward domain of sweet taste. 4. Both groups had improvements in the psychological aspects related to eating behaviour. 5. GLP-1 and PYY were significantly higher in the DJBL group at six months compared to the control group. 6. Late-phase glucose and insulin concentrations were significantly lower in the DJBL group compared to the control group. Conclusion: Weight loss was shown to be primarily due to caloric reduction with no superior benefit caused by the bypass of the proximal small bowel. The DJBL also resulted in a modest change in food preferences. No changes were observed in any of taste domains. There was a higher concentration of GLP-1 and PYY at six months, as well as lower glucose and insulin concentrations in the DJBL group. My findings suggest that the bypass of the proximal intestine in RYGB might not be the component of the surgery that underlies the reduction in energy intake and change in food preferences.
White on black: A white-box-oriented approach for selecting black box-generated test cases
Creators: name: Chen, Tsong
name: Poon, P. L.
name: Tang, S. F.
name: Yu, Y. T.
Publisher: name: Swinburne
Year: 2024

description: Many useful test case construction methods that are based on important aspects of the specification have been proposed in the literature. A comprehensive test suite thus obtained is often very large and yet is non-redundant with respect to the aspects identified from the specification. This paper addresses the problem of selecting a subset of test cases from such a test suite. We propose the use of white box criteria to select test cases from the initial black-box-generated test suite. We illustrate our ideas with examples and demonstrate the viability and benefits of our approach by means of a case study.
Distinct change in zooplankton zoogeography in high-Arctic Isfjorden, Svalbard
Creators: name: Stürzinger, Vegard
name: Søreide, Janne
name: Daase, Malin
Publisher: name: Munin UiT
Year: 2023
Subjects: Atlantification
Svalbard
Zoogeography
European Arctic
Time series

description: Ongoing global warming in air and sea temperatures are changing the environment at a drastic pace. Arctic fjords are becoming warmer and fjord ice is decreasing and, in some cases, not forming. The zooplankton community is proposed to shift from an Arctic to a more boreal one – popularly termed “Atlantification” of the zooplankton community in the European Arctic. The extent and rate of change is challenging to determine due to the lack of longer zooplankton time series with sufficient species and zoogeographical resolution. The Isfjorden Marine Observatory Svalbard (IMOS) is a high-Arctic zooplankton time series with seasonal resolution along a transect from warm Atlantic influenced to cold Arctic water masses. Using zoogeography defined in the literature and customized for Isfjorden, determined from multivariate zooplankton and environmental analyses, in the period 2011-2022, zooplankton species were defined to be Arctic, widespread, Atlantic, or not defined. Multivariate and literature zoogeography revealed that there is ongoing Atlantification in Isfjorden, except in the innermost part (Billefjorden) which is protected by a shallow sill preventing Atlantic water masses from entering. A clear environmental and zooplankton zoogeographical gradient from the warmer fjord mouth to the inner cold Arctic sill fjord were found. According to literature zoogeography there are between ~34-45% Atlantic and widespread species in the outermost station, while in Billefjorden there are less than ~30%. According to the multivariate zoogeography there are 75-85% Atlantic species on the outermost station and 60% in Billefjorden. Many of the species defined in this thesis could be beneficial for management as it allows us to look at the whole zooplankton community and not only a few species for signs of Atlantification or Arctic resilience. Central is also an evaluation of data handling in modern science, and with the escalation of cloud storage, it is paramount to make data available. This increases any value data collected has and enables large-scale biological data analysis.
Regulation of cellular senescence in COPD small airway fibroblasts
Creators: name: Wrench, Catherine Louise
Publisher: name: Imperial College London
Year: 2019

description: Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory lung condition associated with small airway obstruction due to fibrosis and airway collapse (small airways disease (SAD)). COPD is associated with cellular senescence; by which cells undergo irreversible growth arrest and can be induced by stressors such as oxidative stress. Senescent cells are not inert but remain metabolically active expressing the senescence-associated secretory phenotype (SASP), that induces inflammation and tissue remodelling. Fibroblasts deposit extracellular matrix (ECM) and may facilitate SAD. Senescence is associated with a pro-fibrotic fibroblast phenotype in fibrotic lung disease, however only parenchymal-derived fibroblasts have been studied in this context in COPD. Whether small airway-derived fibroblasts (SAF) are senescent in COPD and how this contributes to SAD is largely unknown. SAF from age-matched non-smokers, smokers and COPD patients were examined for their senescent and fibrotic characteristics, and whether oxidative stress induces this phenotype. To better understand the pathways involved in driving senescence and if this is linked to a fibrotic phenotype, a senescent-enriched population of COPD SAF was isolated using fluorescence-activated cell sorting (FACS) and studied further. Markers of senescence were increased in COPD SAF compared to non-smoker SAF. These included SASP mediators that can promote fibrosis. COPD SAF trended towards depositing more collagen type III compared to non-smoker SAF. Oxidative stress-induced cellular senescence and pro-fibrotic mediator expression but did not elevate ECM expression in this model. Using FACS, a senescence-enriched population of SAF was isolated, as determined by senescence marker expression and displayed increased ECM markers expression and a SASP that may influence fibrosis. This study suggests COPD SAF are senescent and possibly associated with fibrotic properties. Oxidative stress can mediate this senescent phenotype but needs further exploration for its contribution to fibrosis. Targeting of cellular senescence in SAF may limit fibrosis and therefore the progression of SAD.
XXX_XXX
Creators: name: Wilson, Steven Ray
Publisher: name: figshare
Year: 2015
Subjects: Cancer
Biological Techniques

description: Targeted proteomics through nanoLC-MS/MS has the potential to measure several proteins in a complex sample in reasonable analysis time. However, to be a contender to existing methods; WB and ELISA, sample preparation has to be minimized, sensitivity high and analysis time short. We compared four commercially available nanoLC columns with various morphologies (Chromolith® (silica monolith), PepMap™ (porous particles), Accucore™ (solid core particles), and PepSwift™ (organic monolith)) for determination of Wnt/beta-catenin related pathway proteins in unfractionated/undepleted cell samples with gradients of 30 minutes. We found that the solid core particle packed column outperformed the other columns in terms of peak capacity (~190 at 50% peak height in 30 minutes). The average LC retention time shift was also least for the two particle based columns (<1 minute), whereas the monoliths were more prone to retention time instability when sample complexity increased. This will affect selectivity of identifications in a complex background, and effectively reduce assay robustness. Of the particle-packed columns, the solid core particle column had the best retention time repeatability in complex samples (1 % RSD). Only small difference in sensitivity was observed, even if the flow-rate was altered in the range of 200 – 600 nL/min. With the solid core particle packed column, Wnt/beta-catenin signal pathway members beta-catenin and GSK3beta were easily identified in non-fractionated samples and could be measured precisely (below 10 % variation) in complex backgrounds using SILAC labeled cell lines as internal standard which corrects for each sample preparation step and LC-MS variation. However, less abundant pathway proteins such as AXIN2 or TNKS2 could not be detected, perhaps calling for more narrow LC columns for fast trace analysis.
Combined musculoskeletal and finite element modelling of the lumbar spine and lower limbs
Creators: name: Favier, Clement David
Publisher: name: Imperial College London
Year: 2019

description: Bone health deterioration is a major public health issue increasing the risk of fragility fracture with a substantial associated psychosocioeconomic impact. In the lumbar spine, physical deconditioning associated with ageing and chronic pain is a potential promoter of bone structural degradation. General guidelines for the limitation of bone loss and the management of pain have been issued, prescribing a healthy lifestyle and a minimum level of physical activity. However, there is no specific recommendation regarding targeted activities that can effectively maintain lumbar spine bone health in populations at risk. The aim of this thesis was to develop a new predictive computational modelling framework for the study of bone structural adaptation to healthy and pathological conditions in the lumbar spine. The approach is based on the combination of a musculoskeletal model of the lumbar spine and lower limbs with structural finite element models of the lumbar vertebrae. These models are built with bone and muscle geometries derived from healthy individuals. Based on daily living activities, musculoskeletal simulations provide physiological loading conditions to the finite element models. Cortical and trabecular bone are modelled with shell and truss elements whose thicknesses and radii are adapted to withstand the physiological mechanical environment using a strain driven optimisation algorithm. This modelling framework allows to generate healthy bone architecture when a loading envelope representative of a healthy lifestyle is applied to the vertebrae, and identify influential activities. Prediction of bone remodelling under altered loading scenarios characteristic of lumbar pathologies can also be achieved. The modelling approach developed in this thesis is a powerful tool for the investigation of bone remodelling in the lumbar spine. Preliminary results indicate that locomotion activities are insufficient to maintain lumbar spine bone health. Specific recommendations to limit the effect of physical deconditioning related to muscle weakening back pain are suggested. The approach is also promising for the investigation of other lumbar pathologies such as age related osteoporosis and scoliosis.
Evaluating the impact of DNS and HTTP session characteristics on consumer ISP web traffic
Creators: name: But, Jason
name: Keller, Urs
name: Armitage, Grenville
Publisher: name: Swinburne
Year: 2024

description: Web caches are generally considered useful because they reduce replication of network traffic flowing from original content sources. In this paper we experimentally characterise the network and transport layer consequences of web caching in the consumer ISP context. We instrumented a small number of Australian, broadband-attached homes to collect round-trip time (RTT) and hop count statistics for their HTTP/TCP sessions, and collect DNS lookup statistics associated with each HTTP exchange.We estimated the impact of DNS lookup delays on overall HTTP session times, and use our RTT and hop count statistics to show that consumer ISPs would benefit greatly from local caching, particularly in Australia where speed of light delays have a large impact on session times when retrieving international content.
Distribution, abundance and length of macrozooplankton species in Svalbard fjords
Creators: name: Lunde, Andreas
name: Søreide, Janne
name: Vanhatalo, Jarno
Publisher: name: Zenodo
Year: 2023
Subjects: Svalbard
Macrozooplankton
Fjords
Amphipod
Krill
Arctic
Distribution
Abundance
Length distribution

description: Macrozooplankton is an understudied size class of plankton in the Arctic, but even though species composition is similar around the Svalbard archipelago, the relative composition can act as a proxy for climate change. This study investigated if the fjords around Svalbard are similar in species composition and if any differences could be explained. 11 fjords were sampled with a focus on common and indicator species in terms of abundance, relative composition and length distribution. I found low presence of T.libellula and high abundances of T.abyssorum and T.inermis. T.abyssorum was the only species whos abundance was significantly correlated to water mass (Atlantic water), but T.libellula, T.abyssorum, T.inermis, T.longicaudata and A.digitale all showed significant differences in length distribution. This study provides further understanding of species composition in the different high Arctic fjords around Svalbard.
D4.3 - Review of tool support for ontology management and ontology-based data documentation
Creators: name: Skjæveland, Martin
name: Slaughter, Laura
name: Kindermann, Christian
Publisher: name: Zenodo
Year: 2022
Subjects: Ontology Engineering
Toolkit
Ontology Tools
Software Systems

description: This report provides a landscape analysis of software systems for ontology engineering. We collect software systems that are said to be used in practice and compile them in an index providing information about their homepage, documentation, and other publicly available materials.Furthermore, we classify collected software systems according to high-level categories that have been proposed in the report on “Ontology Ecosystem Specification”. This categorisation can be used as a first guide by practitioners to search and select software systems for ontology engineering tasks that are most suitable for their needs. Lastly, we conduct a desk review analysis of the collected software systems considering novel challenges arising from application domains as put forward by the report on “Requirements on ontology tools and ontologies and criteria for selection of further cases”. We find that some areas in ontology engineering seem to be lacking in terms of tool support, e.g., modularisation, conceptual modelling, and ontology reuse. However, our results also suggests that for many challenges arising in practice there is often some kind of tool support that may prove to be useful for addressing said challenges.
Tracing water masses and pollution in the Southern Ocean using neodymium and lead isotopes
Creators: name: Griffiths, Alexander Matthew
Publisher: name: Imperial College London
Year: 2019

description: Trace elements and their isotopes play a vital role in the ocean as participants in, and tracers of, processes of interest to climate change and environmental pollution. This thesis focuses on the use of isotopic variations in neodymium (Nd) and lead (Pb) to understand the cycling of these elements in the Southern Ocean. Neodymium isotopes have been used as a palaeo-proxy to understand changes in Antarctic Bottom Water (AABW) circulation through time. The biogeochemical processes controlling Nd in seawater, however, remain under-constrained due to a paucity of modern observations in the Southern Ocean. In chapter 2, Nd isotope and rare earth element (REE) data are presented for the Wilkes Land continental margin. In this region, AABW exhibits a distinct εNd signature that is intermediate between Atlantic and Pacific sector AABW. The REE data confirm that the εNd signature is not caused by distinct local continental inputs but by mixing of advected AABW with (modified) Circumpolar Deep Water (CDW). Anthropogenic emissions from mining, smelting and fossil fuel combustion are important sources of oceanic Pb contamination. Seawater Pb isotope data, however, is currently severely limited by analytical challenges such as sample contamination, time-consuming extraction procedures and insufficient instrumental detection limits. In chapter 3, a novel method is presented for the determination of seawater Pb isotope compositions and concentrations. The method encompasses solid-phase extraction of Pb from seawater with Nobias chelate PA-1 resin followed by multi-collector inductively coupled mass spectrometry (MC-ICP-MS) analyses using a 207Pb-204Pb double-spike to correct for instrumental mass discrimination. When compared to an established double-spike procedure that employs thermal ionisation mass spectrometry (TIMS), the results are unbiased by systematic error and demonstrates improved precision for the and the minor 204Pb ratios (by about a factor of 2). In chapter 4, the new method is applied to seawater samples from the Australian sector of the Southern Ocean to assess the sources and processes governing the distribution of Pb in this region. Surface waters exhibit a high fraction of anthropogenic Pb (~30–50%). Reversible scavenging and equilibrium exchange are the dominant processes responsible for the vertical transport of this anthropogenic Pb to deeper waters. These processes may account for ~80% of the observed dissolved Pb isotope in the intermediate depth waters at the Polar Front. Overall, the thesis highlights the essential role that isotope analyses play in deconvolving the processes responsible for the biogeochemical cycling of Nd and Pb in the ocean.
title: Rebe :
title: Richtlinien für die Durchführung der Prüfung auf Unterscheidbarkeit, Homogenität und Beständigkeit.
titleType: Subtitle
Creators: name: (:Unas)
Publisher: name: Unknown
Year: 2008

description: Zweck dieser Richtlinien („Prüfungsrichtlinien“) ist es, die in der Allgemeinen Einführung (Dokument TG/1/3) und deren verbundenen TGP-Dokumenten enthaltenen Grundsätze in detaillierte praktische Anleitung für die harmonisierte Prüfung der Unterscheidbarkeit, der Homogenität und der Beständigkeit (DUS) umzusetzen und insbesondere geeignete Merkmale für die DUS-Prüfung und die Erstellung harmonisierter Sortenbeschreibungen auszuweisen.
XXX_XXX
Creators: name: Wilson, Steven Ray
Publisher: name: figshare
Year: 2016
Subjects: Cancer
Biological Techniques

description: Targeted proteomics through nanoLC-MS/MS has the potential to measure several proteins in a complex sample in reasonable analysis time. However, to be a contender to existing methods; WB and ELISA, sample preparation has to be minimized, sensitivity high and analysis time short. We compared four commercially available nanoLC columns with various morphologies (Chromolith® (silica monolith), PepMap™ (porous particles), Accucore™ (solid core particles), and PepSwift™ (organic monolith)) for determination of Wnt/beta-catenin related pathway proteins in unfractionated/undepleted cell samples with gradients of 30 minutes. We found that the solid core particle packed column outperformed the other columns in terms of peak capacity (~190 at 50% peak height in 30 minutes). The average LC retention time shift was also least for the two particle based columns (<1 minute), whereas the monoliths were more prone to retention time instability when sample complexity increased. This will affect selectivity of identifications in a complex background, and effectively reduce assay robustness. Of the particle-packed columns, the solid core particle column had the best retention time repeatability in complex samples (1 % RSD). Only small difference in sensitivity was observed, even if the flow-rate was altered in the range of 200 – 600 nL/min. With the solid core particle packed column, Wnt/beta-catenin signal pathway members beta-catenin and GSK3beta were easily identified in non-fractionated samples and could be measured precisely (below 10 % variation) in complex backgrounds using SILAC labeled cell lines as internal standard which corrects for each sample preparation step and LC-MS variation. However, less abundant pathway proteins such as AXIN2 or TNKS2 could not be detected, perhaps calling for more narrow LC columns for fast trace analysis.