cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
GC/MS profiling, in vitro anti-leptospiral and haemolytic activities of Boesenbergia rotunda (L.) Mansf. used as a medicinal plant by Nicobarese of Andaman and Nicobar Islands
Creators: name: M. Punnam Chander
name: K. Vinod Kumar
name: Lall, Chandan
name: R. Vimal Raj
name: P. Vijayachari
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Biochemistry
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Pharmacology
subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: Immunology
subject: FOS: Clinical medicine

description: Leaves of the plant Boesenbergia rotunda are used by the Nicobarese tribe of Andaman and Nicobar Islands, India, to prepare traditional medicine for treating fever, headache and body ache. In the present investigation, methanol fraction of these leaves were analysed by GC/MS that revealed the presence of 25 compounds. The anti-leptospiral activity of methanol crude extract was determined by both microdilution and macrodilution methods. The MICs of the extract were tested against 24 pathogenic leptospiral strains and ranged between 62.5–125 μg/mL in both microdilution and macrodilution. The range of MBCs was 250 and 500 μg/mL in macrodilution and microdilution respectively. The crude extract was subjected to cytotoxic studies and found to have negligible or no haemolytic activity, exhibiting IC50 values of greater than 4 mg/mL. Further in vivo studies are needed to investigate the pharmacological and toxicological properties of Boesenbergia rotunda, before it can be considered as a new anti-leptospiral agent.
Helpful or harmful? Exploring the impact of social media usage on intimate relationships
Creators: name: Whiteside, N.
name: Aleti, T.
name: Pallant, Jason
name: Zeleznikow, J.
Publisher: name: Swinburne
Year: 2024
Subjects: classificationCode: 350699
subject: Marketing not elsewhere classified

description: Internet access in Australia is increasing with the most popular online activity reported by Australians being social media use. The literature is divided as to whether social media use is helpful or harmful to intimate relationships. Recent research from an Australian family counselling provider indicates that social media use is resulting in negative intimate relationship outcomes, echoing recent American research findings. However, other researchers note that social media use can have positive outcomes for intimate relationships and individuals. These different findings prompted this research which focuses on the impact of social media usage on intimate relationships using Facebook as a case study. A survey of 518 Australians examining Facebook usage, relationship satisfaction and household income was undertaken. The results suggest that relationship satisfaction varies based on types of social media usage when controlled for household income. For most respondents, social media usage did not have a negative impact on their relationship. However, results indicated that where their partner was a ‘friend on social media, they were less likely to report concerns relating to social media usage impacting their relationship. It was also found that those living alone or with children on low incomes were more likely to be dissatisfied with their relationships and rely on social media to connect with others. Finally, four types of Facebook usage were identified and mapped against intensity of usage and relationship satisfaction, indicating that the way in which social media is used impacts relationship satisfaction. These findings indicate that social media use is helpful for some intimate relationships and harmful for others based on individual usage and their interaction with their partner via the platform.
The hydrophobic patch directs cyclin B to centrosomes to promote global CDK phosphorylation at mitosis
Creators: name: Souradeep Basu
name: Roberts, Emma L
name: Jones, Andrew W
name: Swaffer, Matthew P
name: Snijders, Ambrosius P
name: Nurse, Paul
Publisher: name: The Francis Crick Institute
Year: 2020
Subjects: Model organisms
Biochemistry & Proteomics
Synthetic Biology
Genetics & Genomics
Cell Biology
Cell Cycle & Chromosomes
Chemical Biology & High Throughput
Computational & Systems Biology

description: The cyclin-dependent kinases (CDKs) are the major cell-cycle regulators that phosphorylate hundreds of substrates, controlling the onset of S phase and M phase [1-3]. However, the patterns of substrate phosphorylation increase are not uniform, as different substrates become phosphorylated at different times as cells proceed through the cell cycle [4, 5]. In fission yeast, the correct ordering of CDK substrate phosphorylation can be established by the activity of a single mitotic cyclin-CDK complex [6, 7]. Here, we investigate the substrate-docking region, the hydrophobic patch, on the fission yeast mitotic cyclin Cdc13 as a potential mechanism to correctly order CDK substrate phosphorylation. We show that the hydrophobic patch targets Cdc13 to the yeast centrosome equivalent, the spindle pole body (SPB), and disruption of this motif prevents both centrosomal localization of Cdc13 and the onset of mitosis but does not prevent S phase. CDK phosphorylation in mitosis is compromised for approximately half of all mitotic CDK substrates, with substrates affected generally being those that require the highest levels of CDK activity to become phosphorylated and those that are located at the SPB. Our experiments suggest that the hydrophobic patch of mitotic cyclins contributes to CDK substrate selection by directing the localization of Cdc13-CDK to centrosomes and that this localization of CDK contributes to the CDK substrate phosphorylation necessary to ensure proper entry into mitosis. Finally, we show that mutation of the hydrophobic patch prevents cyclin B1 localization to centrosomes in human cells, suggesting that this mechanism of cyclin-CDK spatial regulation may be conserved across eukaryotes.
Concise Synthesis of (±)-Clopidogrel via Carboxylation of Benzylamine with CO2
Creators: name: V. Venkataramasubramanian
name: Sudalai, Arumugam
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Engineering
subject: Information and Computing Sciences
subject: Biological Sciences
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Biotechnology
subject: Microbiology
subject: FOS: Biological sciences
subject: Biochemistry
subject: Biophysics

description: A concise and efficient synthesis of (±)-clopidogrel, an antithrombotic agent, is achieved by inserting CO2 at the benzylic position as the key reaction without using any toxic transition metals. The overall yield of the synthetic process is 38% and the salient features include operationally simple process chemistry and fewer steps.
Butler enables rapid cloud-based analysis of thousands of human genomes.
Creators: name: Yakneen, Sergei
name: Waszak, Sebastian M
name: PCAWG Technical Working Group
name: Gertz, Michael
name: Korbel, Jan O
name: PCAWG Consortium
Publisher: name: The Francis Crick Institute
Year: 2020
Subjects: Genetics & Genomics
Human Biology & Physiology
Ecology,Evolution & Ethology
Tumour Biology
Computational & Systems Biology

description: We present Butler, a computational tool that facilitates large-scale genomic analyses on public and academic clouds. Butler includes innovative anomaly detection and self-healing functions that improve the efficiency of data processing and analysis by 43% compared with current approaches. Butler enabled processing of a 725-terabyte cancer genome dataset from the Pan-Cancer Analysis of Whole Genomes (PCAWG) project in a time-efficient and uniform manner.
Using the GB2 Income Distribution
Creators: name: Chotikapanich, Duangkamon
name: Griffiths, William
name: Hajargasht, Reza
name: Karunarathne, Wasana
name: Rao, D.
Publisher: name: Swinburne
Year: 2024

description: To use the generalized beta distribution of the second kind (GB2) for the analysis of income and other positively skewed distributions, knowledge of estimation methods and the ability to compute quantities of interest from the estimated parameters are required. We review estimation methodology that has appeared in the literature, and summarize expressions for inequality, poverty, and pro-poor growth that can be used to compute these measures from GB2 parameter estimates. An application to data from China and Indonesia is provided.
Anti-PD-1 immunotherapy leads to tuberculosis reactivation via dysregulation of TNF-α.
Creators: name: Liku B Tezera
name: Bielecka, Magdalena K
name: Ogongo, Paul
name: Walker, Naomi F
name: Ellis, Matthew
name: Garay-Baquero, Diana J
name: Thomas, Kristian
name: Reichmann, Michaela T
name: Johnston, David A
name: Wilkinson, Katalin Andrea
name: Ahmed, Mohamed
name: Jogai, Sanjay
name: Suwan N Jayasinghe
name: Wilkinson, Robert J
name: Mansour, Salah
name: Thomas, Gareth J
name: Ottensmeier, Christian H
name: Leslie, Alasdair
name: Elkington, Paul T
Publisher: name: The Francis Crick Institute
Year: 2020
Subjects: subject: Model organisms
subject: Infectious Disease
subject: Human Biology & Physiology
subject: Immunology
subject: FOS: Clinical medicine

description: Previously, we developed a 3-dimensional cell culture model of human tuberculosis (TB) and demonstrated its potential to interrogate the host-pathogen interaction (Tezera et al, 2017). Here, we use the model to investigate mechanisms whereby immune checkpoint therapy for cancer paradoxically activates TB infection. In patients, PD-1 is expressed in Mycobacterium tuberculosis (Mtb)-infected lung tissue but absent in areas of immunopathology. In the microsphere model, PD-1 ligands are up-regulated by infection, and the PD-1/PD-L1 axis is further induced by hypoxia. Inhibition of PD-1 signalling increases Mtb growth, and augments cytokine secretion. TNF-α is responsible for accelerated Mtb growth, and TNF-α neutralisation reverses augmented Mtb growth caused by anti-PD-1 treatment. In human TB, pulmonary TNF-α immunoreactivity is increased and circulating PD-1 expression negatively correlates with sputum TNF-α concentrations. Together, our findings demonstrate that PD-1 regulates the immune response in TB, and inhibition of PD-1 accelerates Mtb growth via excessive TNF-α secretion.
Facile Denitrosation of Cyclic N-Nitrosamines with Hydrazoic Acid
Creators: name: S. Ponnuswamy
name: A. Akila
name: D. Kiruthiga Devi
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Cancer
subject: Developmental Biology
subject: Mathematics
subject: FOS: Mathematics
subject: Biological Sciences
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Biotechnology
subject: Cell Biology
subject: Microbiology
subject: FOS: Biological sciences
subject: Biochemistry

description: A simple and facile method for the denitrosation of cyclic N-nitrosamines using HN3 (concentrated H2SO4 + NaN3) is reported. In this method, limited usage of this reagent does not affect the carbonyl group.
Experimental investigation on flexural properties of FDM processed Nylon 12 parts using RSM
Creators: name: Kamoona, Salam Nori
name: Masood, Syed
name: Mohamed, Omar Ahmed
Publisher: name: Swinburne
Year: 2024

description: Fused deposition modelling (FDM) is one of the leading additive manufacturing technologies for producing plastic parts in a layer by layer manner d from a digital file. The actual levels of processed parameters of the manufactured parts using fused deposition modelling (FDM) are considered the major obstruction to optimize the mechanical properties. As a result of the FDM parameters and the interaction among them the parts’ mechanical properties of the manufactured parts are affected, thus, many experiments have been dedicated to find the proper parameter setting. This paper presents a study on the influence of three FDM process parameters (air gap, raster angle, and build orientation) on the flexural strength of the FDM Nylon 12 manufactured parts. Response surface methodology (RSM) is used as a statistical technique to analyse results. Analysis of variance (ANOVA) method is also used to test the significance of parameters. The results indicate that the air gap and raster angle are the most influential parameters affecting the flexural strength.
Concise Synthesis of (±)-Clopidogrel via Carboxylation of Benzylamine with CO2
Creators: name: V. Venkataramasubramanian
name: Sudalai, Arumugam
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Engineering
subject: Information and Computing Sciences
subject: Biological Sciences
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Biotechnology
subject: Microbiology
subject: FOS: Biological sciences
subject: Biochemistry
subject: Biophysics

description: A concise and efficient synthesis of (±)-clopidogrel, anti-thrombotic agent, is achieved by employing the insertion of CO2 at the benzylic position as the key reaction without using any toxic transition metals. The overall yield of the synthetic process is 38% and the salient features include operationally simple process chemistry and less number of steps.
Facile Denitrosation of Cyclic N-Nitrosamines with Hydrazoic Acid
Creators: name: S. Ponnuswamy
name: A. Akila
name: D. Kiruthiga Devi
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Cancer
subject: Developmental Biology
subject: Mathematics
subject: FOS: Mathematics
subject: Biological Sciences
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Biotechnology
subject: Cell Biology
subject: Microbiology
subject: FOS: Biological sciences
subject: Biochemistry

description: A simple and facile method for the denitrosation of cyclic N-nitrosamines using HN3 (Conc.H2SO4 +NaN3) is reported. In this method limited usage of this reagent does not affect the carbonyl group.
A brief overview of solar and wind energy in Libya: Current trends and the future development
Creators: name: Mohamed, Omar Ahmed
name: Masood, Syed
Publisher: name: Swinburne
Year: 2024

description: Libya is one of the oil exporters and natural gas exporters to become one of the top lists of primary energy sources in the world. On the other hand, Libya, like other countries in the world suffers from high energy consumption, high conventional energy prices and environment issues, combined with rapid demand growth. As a result, the Renewable Energy Authority of Libya (REAOL) has been founded to promote the development of renewable energy in Libya to increase the utilization of renewable energy from 6% to 10% by the year of 2020. This study corresponds to a revision of the current scenario of energy resources; provide future potential of renewable energy resources in Libya and implementation of the future projects for the utilization of renewable energy. This study shows that there is huge potential for renewable energy in Libya, especially solar and wind. The Libyan government will have to be more aggressive targets to promotion of renewable energy to achieve environmental sustainability in Libya.
Promoting the Use of Green Roofs on Street-Level Surfaces to Improve Public Awareness about Stormwater Management in Boston
Creators: name: Andreoni, Victor
name: Sullivan, Oliver
name: Payne, Kevin
Publisher: name: QUBES Educational Resources
Year: 2025

description: Stormwater runoff is a problem in the City of Boston due to the urbanization of the area. The goal of our project was to determine the technical and financial feasibility of installing green roofs on street-level surfaces in the City of Boston with the purpose of raising public awareness and encouraging a change in the public’s behavior with respect to stormwater runoff. We
conducted interviews with representatives from similar projects in other cities and performed an observational study to determine the possibility of installing green roofs on bus stops for the purpose of raising public awareness. Our research led to a set of recommendations involving the technical, financial, informational, and visual aspects for the design of street-level green roofs on bus stops.
A guide to selecting high-performing antibodies for STXBP1 (UniProt ID: P61764) for use in western blot, immunoprecipitation, and immunofluorescence
Creators: name: Ayoubi, Riham
name: Alende, Charles
name: Fotouhi, Maryam
name: Gonzalez Bolivar, Sara
name: Laflamme, Carl
name: NeuroSGC/YCharOS/EDDU collaborative group
name: ABIF consortium
Publisher: name: Zenodo
Year: 2024

description: A peer-reviewed antibody characterization article corresponding to this Zenodo preprint is openly available at F1000Research: https://doi.org/10.12688/f1000research.160174.1

This report presents a guide to selecting high-quality commercial antibodies against STXBP1 in Western blot, immunoprecipitation and immunofluorescence, using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls.

This study was funded in part by the Simons Foundation Autism Research Initiative (SFARI), an organization that funds innovative research to enhance the understanding, diagnosis and treatment of autism spectrum disorders.
Getting elections right? Measuring electoral integrity
Creators: name: Ham, Carolien Van
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Plant Biology
subject: FOS: Biological sciences
subject: 111714 Mental Health
subject: FOS: Health sciences
subject: Science Policy
subject: Cancer
subject: Information and Computing Sciences
subject: Chemistry
subject: Biotechnology
subject: Medicine
subject: Biochemistry

description: Holding elections has become a global norm. Unfortunately, the integrity of elections varies strongly, ranging from “free and fair” elections with genuine contestation to “façade” elections marred by manipulation and fraud. Clearly, electoral integrity is a topic of increasing concern. Yet electoral integrity is notoriously difficult to measure, and hence taking stock of the available data is important. This article compares cross-national data sets measuring electoral integrity. The first part evaluates how the different data sets (a) conceptualize electoral integrity, (b) move from concepts to indicators, and (c) move from indicators to data. The second part analyses how different data sets code the same elections, seeking to explain the sources of disagreement about electoral integrity. The sample analysed comprises 746 elections in 95 third and fourth wave regimes from 1974 until 2009. I find that conceptual and measurement choices affect disagreement about election integrity, and also find that elections of lower integrity and post-conflict elections generate higher disagreement about election integrity. The article concludes with a discussion of results and suggestions for future research.
A single cell transcriptome atlas of the developing zebrafish hindbrain.
Creators: name: Tambalo, Monica
name: Mitter, Richard
name: Wilkinson, David G
Publisher: name: The Francis Crick Institute
Year: 2020
Subjects: subject: Neurosciences
subject: FOS: Clinical medicine
subject: Model organisms
subject: Tumour Biology
subject: Genetics & Genomics
subject: Developmental Biology
subject: Genome Integrity & Repair
subject: Human Biology & Physiology
subject: Stem Cells
subject: Chemical Biology & High Throughput
subject: Computational & Systems Biology

description: Segmentation of the vertebrate hindbrain leads to the formation of rhombomeres, each with a distinct anteroposterior identity. Specialised boundary cells form at segment borders that act as a source or regulator of neuronal differentiation. In zebrafish, there is spatial patterning of neurogenesis in which non-neurogenic zones form at boundaries and segment centres, in part mediated by Fgf20 signaling. To further understand the control of neurogenesis, we have carried out single cell RNA sequencing of the zebrafish hindbrain at three different stages of patterning. Analyses of the data reveal known and novel markers of distinct hindbrain segments, of cell types along the dorsoventral axis, and of the transition of progenitors to neuronal differentiation. We find major shifts in the transcriptome of progenitors and of differentiating cells between the different stages analysed. Supervised clustering with markers of boundary cells and segment centres, together with RNA-seq analysis of Fgf-regulated genes, has revealed new candidate regulators of cell differentiation in the hindbrain. These data provide a valuable resource for functional investigations of the patterning of neurogenesis and the transition of progenitors to neuronal differentiation.
DNA copy number motifs are strong and independent predictors of survival in breast cancer.
Creators: name: Pladsen, Arne V
name: Nilsen, Gro
name: Rueda, Oscar M
name: Aure, Miriam R
name: Borgan, Ørnulf
name: Liestøl, Knut
name: Vitelli, Valeria
name: Frigessi, Arnoldo
name: Langerød, Anita
name: Mathelier, Anthony
name: OSBREAC
name: Engebråten, Olav
name: Kristensen, Vessela
name: Wedge, David C
name: Loo, Peter Van
name: Caldas, Carlos
name: Anne-Lise Børresen-Dale
name: Russnes, Hege G
name: Lingjærde, Ole Christian
Publisher: name: The Francis Crick Institute
Year: 2020
Subjects: Genetics & Genomics
Human Biology & Physiology
Ecology,Evolution & Ethology
Tumour Biology
Computational & Systems Biology

description: Somatic copy number alterations are a frequent sign of genome instability in cancer. A precise characterization of the genome architecture would reveal underlying instability mechanisms and provide an instrument for outcome prediction and treatment guidance. Here we show that the local spatial behavior of copy number profiles conveys important information about this architecture. Six filters were defined to characterize regional traits in copy number profiles, and the resulting Copy Aberration Regional Mapping Analysis (CARMA) algorithm was applied to tumors in four breast cancer cohorts (n = 2919). The derived motifs represent a layer of information that complements established molecular classifications of breast cancer. A score reflecting presence or absence of motifs provided a highly significant independent prognostic predictor. Results were consistent between cohorts. The nonsite-specific occurrence of the detected patterns suggests that CARMA captures underlying replication and repair defects and could have a future potential in treatment stratification.
Regulation of the RNAPII pool is integral to the DNA damage response.
Creators: name: Vidaković, Ana Tufegdžić
name: Mitter, Richard
name: Kelly, Gavin P
name: Neumann, Michelle
name: Harreman, Michelle
name: Rodríguez-Martínez, Marta
name: Herlihy, Anna
name: Weems, Juston C
name: Boeing, Stefan
name: Encheva, Vesela
name: Gaul, Liam
name: Milligan, Laura
name: Tollervey, David
name: Conaway, Ronald C
name: Conaway, Joan W
name: Snijders, Ambrosius P
name: Stewart, Aengus
name: Svejstrup, Jesper Q
Publisher: name: The Francis Crick Institute
Year: 2020
Subjects: Model organisms
Biochemistry & Proteomics
Tumour Biology
Gene Expression
Genetics & Genomics
Genome Integrity & Repair
Chemical Biology & High Throughput
Computational & Systems Biology

description: In response to transcription-blocking DNA damage, cells orchestrate a multi-pronged reaction, involving transcription-coupled DNA repair, degradation of RNA polymerase II (RNAPII), and genome-wide transcription shutdown. Here, we provide insight into how these responses are connected by the finding that ubiquitylation of RNAPII itself, at a single lysine (RPB1 K1268), is the focal point for DNA-damage-response coordination. K1268 ubiquitylation affects DNA repair and signals RNAPII degradation, essential for surviving genotoxic insult. RNAPII degradation results in a shutdown of transcriptional initiation, in the absence of which cells display dramatic transcriptome alterations. Additionally, regulation of RNAPII stability is central to transcription recovery-persistent RNAPII depletion underlies the failure of this process in Cockayne syndrome B cells. These data expose regulation of global RNAPII levels as integral to the cellular DNA-damage response and open the intriguing possibility that RNAPII pool size generally affects cell-specific transcription programs in genome instability disorders and even normal cells.
Intel and its relation with COVID 19
Creators: name: Victoria, Artur
Publisher: name: Zenodo
Year: 2020

description: Pre-Conclusion The ultimate purpose of intelligence it is the dissemination. The adequate diffusion of Intelligence can be judged from the point of view of its priority, taking into account the following criteria. 1- Information Such as intelligence must be placed in a timely manner in the hands of the person who will primarily use it, in order to allow its evaluation and interpretation, the formulation of plans and the initiation of action under existing conditions, before the picture changes. 2- Intelligence. If the information is disseminated without processing, it can be clearly indicated that said information has not been evaluated. If security permits, the source of the information will be identified. 1- Only essential Intelligence of interest to the unit under consideration will be disseminated. 2- The importance and priority of disseminated intelligence will be carefully considered. The means of communication chosen for broadcasting must be such that they produce the least intelligence in the operation of the communications system. Conclusion Effectively what has been noticed in this pandemic situation of the COVID19 virus has: 1 - Speculative information and misinformation; 2 - The inexistence of an information service dedicated to epidemics and catastrophes with truly professional characteristics that allow the various operational commands and decision makers to perceive the dangerous situation, its permanent evolution. This intelligence information service must have the characteristics of this mission like: - Detection of the epidemic, catastrophe or fire-starting forest fires; - Operational intelligence staff in the affected or potentially affected areas; - Professional intelligence work through the methods succinctly proposed for its dissemination to the operational posts, operational commands and or policy makers. I therefore agree with my esteemed General that this COVID 19is a matter of national security and as such it should be dealt with in the future, in cooperation with international counterparts.
Butyrophilin-2A1 directly binds germline-encoded regions of the Vγ9Vδ2 TCR and is essential for phosphoantigen sensing.
Creators: name: Mohindar M Karunakaran
name: Willcox, Carrie R
name: Mahboob Salim
name: Paletta, Daniel
name: Fichtner, Alina S
name: Noll, Angela
name: Starick, Lisa
name: Nöhren, Anna
name: Begley, Charlotte R
name: Berwick, Katie A
name: Chaleil, Raphaël AG
name: Pitard, Vincent
name: Déchanet-Merville, Julie
name: Bates, Paul A
name: Kimmel, Brigitte
name: Knowles, Timothy J
name: Kunzmann, Volker
name: Walter, Lutz
name: Jeeves, Mark
name: Fiyaz Mohammed
name: Willcox, Benjamin E
name: Herrmann, Thomas
Publisher: name: The Francis Crick Institute
Year: 2020
Subjects: Structural Biology & Biophysics
Computational & Systems Biology

description: Vγ9Vδ2 T cells respond in a TCR-dependent fashion to both microbial and host-derived pyrophosphate compounds (phosphoantigens, or P-Ag). Butyrophilin-3A1 (BTN3A1), a protein structurally related to the B7 family of costimulatory molecules, is necessary but insufficient for this process. We performed radiation hybrid screens to uncover direct TCR ligands and cofactors that potentiate BTN3A1's P-Ag sensing function. These experiments identified butyrophilin-2A1 (BTN2A1) as essential to Vγ9Vδ2 T cell recognition. BTN2A1 synergised with BTN3A1 in sensitizing P-Ag-exposed cells for Vγ9Vδ2 TCR-mediated responses. Surface plasmon resonance experiments established Vγ9Vδ2 TCRs used germline-encoded Vγ9 regions to directly bind the BTN2A1 CFG-IgV domain surface. Notably, somatically recombined CDR3 loops implicated in P-Ag recognition were uninvolved. Immunoprecipitations demonstrated close cell-surface BTN2A1-BTN3A1 association independent of P-Ag stimulation. Thus, BTN2A1 is a BTN3A1-linked co-factor critical to Vγ9Vδ2 TCR recognition. Furthermore, these results suggest a composite-ligand model of P-Ag sensing wherein the Vγ9Vδ2 TCR directly interacts with both BTN2A1 and an additional ligand recognized in a CDR3-dependent manner.
Cep55 promotes cytokinesis of neural progenitors but is dispensable for most mammalian cell divisions
Creators: name: Tedeschi, Antonio
name: Almagro, Jorge
name: Renshaw, Matthew J
name: Messal, Hendrik A
name: Behrens, Axel
name: Petronczki, Mark
Publisher: name: The Francis Crick Institute
Year: 2020
Subjects: Model organisms
Imaging
Tumour Biology
Gene Expression
Genetics & Genomics
Genome Integrity & Repair
Stem Cells

description: No description supplied
microRNA-mediated noise processing in cells: A fight or a game?
Creators: name: Ferro, Elsi
name: Bena, Chiara Enrico
name: Grigolon, Silvia
name: Bosia, Carla
Publisher: name: The Francis Crick Institute
Year: 2020
Subjects: Structural Biology & Biophysics
Computational & Systems Biology

description: In the past decades, microRNAs (miRNA) have much attracted the attention of researchers at the interface between life and theoretical sciences for their involvement in post-transcriptional regulation and related diseases. Thanks to the always more sophisticated experimental techniques, the role of miRNAs as "noise processing units" has been further elucidated and two main ways of miRNA noise-control have emerged by combinations of theoretical and experimental studies. While on one side miRNAs were thought to buffer gene expression noise, it has recently been suggested that miRNAs could also increase the cell-to-cell variability of their targets. In this Mini Review, we focus on the role of miRNAs in molecular noise processing and on the advantages as well as current limitations of theoretical modelling.
To what extent does the Leiden Manifesto also apply to altmetrics? A discussion of the manifesto against the background of research into altmetrics
Creators: name: Bornmann, Lutz
name: Haunschild, Robin
Publisher: name: figshare
Year: 2015
Subjects: subject: Library and Information Studies
subject: FOS: Media and communications

description: Purpose: Hicks, Wouters, Waltman, de Rijcke, and Rafols (2015) have formulated the so-called Leiden manifesto, in which they have assembled the ten principles for a meaningful evaluation of research on the basis of bibliometric data.Approach: In this work the attempt is made to indicate the relevance of the Leiden manifesto for altmetrics.Results: As shown by the discussion of the ten principles against the background of the knowledge about and the research into altmetrics, the principles also have a great importance for altmetrics and should be taken into account in their application.Originality: Altmetrics is already frequently used in the area of research evaluation. Thus, it is important that the user of altmetrics data knows the relevance of the Leiden manifesto also in this area.
To what extent does the Leiden Manifesto also apply to altmetrics? A discussion of the manifesto against the background of research into altmetrics
Creators: name: Bornmann, Lutz
name: Haunschild, Robin
Publisher: name: figshare
Year: 2016
Subjects: subject: Library and Information Studies
subject: FOS: Media and communications

description: Purpose: Hicks, Wouters, Waltman, de Rijcke, and Rafols (2015) have formulated the so-called Leiden manifesto, in which they have assembled the ten principles for a meaningful evaluation of research on the basis of bibliometric data.Approach: In this work the attempt is made to indicate the relevance of the Leiden manifesto for altmetrics.Results: As shown by the discussion of the ten principles against the background of the knowledge about and the research into altmetrics, the principles also have a great importance for altmetrics and should be taken into account in their application.Originality: Altmetrics is already frequently used in the area of research evaluation. Thus, it is important that the user of altmetrics data knows the relevance of the Leiden manifesto also in this area.
To what extent does the Leiden Manifesto also apply to altmetrics? A discussion of the manifesto against the background of research into altmetrics
Creators: name: Bornmann, Lutz
name: Haunschild, Robin
Publisher: name: figshare
Year: 2015
Subjects: subject: Library and Information Studies
subject: FOS: Media and communications

description: Hicks, Wouters, Waltman, de Rijcke, and Rafols (2015) have formulated the so-called Leiden manifesto, in which they have assembled the ten principles for a meaningful evaluation of research on the basis of bibliometric data. In this work the attempt is made to indicate the relevance of the Leiden manifesto for altmetrics. As shown by the discussion of the ten principles against the background of the knowledge about and the research into altmetrics, the principles also have a great importance for altmetrics and should be taken into account in their application. Especially important points in this connection are: 1) If altmetrics are really to be used for evaluations in the future, they should only be used in the framework of an informed peer review. 2) To obtain informative results, advanced altmetric indicators (normalized indicators) should be used. 3) In an evaluation with the help of altmetrics data, the context of the research unit should be taken into account. Analyses should only be undertaken if they make sense for the unit. 4) The altmetrics data used in an evaluation should be transparent and openly accessible. 5) The presentation of altmetrics data should avoid a misplaced concreteness and false precision, since the underlying data does not permit this. 6) The use of data in research evaluation should be accompanied by continuing scientometric research, in order to optimize altmetric indicators or to develop new ones. Attention must be paid to system effects of indicators, in order e.g. to recognize gaming.
Managing and eradicating wildlife tuberculosis in New Zealand
Creators: name: B Warburton
name: P Livingstone
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: Science Policy
subject: Cancer
subject: Information and Computing Sciences
subject: Biological Sciences
subject: Ecology
subject: FOS: Biological sciences
subject: Molecular Biology

description: Tuberculosis (TB) due to Mycobacterium bovis infection was first identified in brushtail possums (Trichosurus vulpecula) in New Zealand in the late 1960s. Since the early 1970s, possums in New Zealand have been controlled as part of an ongoing strategy to manage the disease in livestock. The TB management authority (TBfree New Zealand) currently implements three strategic choices for disease-related possum control: firstly TB eradication in areas selected for eradication of the disease from livestock and wildlife, secondly Free Area Protection in areas in which possums are maintained at low densities, normally along a Vector Risk Area (VRA) boundary, and thirdly Infected Herd Suppression, which includes the remaining parts of VRA where possums are targeted to minimise the infection risk to livestock. Management is primarily through a range of lethal control options. The frequency and intensity of control is driven by a requirement to reduce populations to very low levels (usually to a trap-catch index below 2%), then to hold them at or below this level for 5–10 years to ensure disease eradication.Lethal possum control is implemented using aerial- and ground-based applications, under various regulatory and operational constraints. Extensive research has been undertaken aimed at improving the efficacy and efficiency of control. Aerial applications use sodium fluoroacetate (1080) bait for controlling possums over extensive and rugged areas of forest that are difficult to access by foot. Ground-based control uses a range of toxins (primarily, a potassium cyanide-based product) and traps. In the last 5 years there has been a shift from simple possum population control to the collection of spatial data on possum presence/absence and relative density, using simple possum detection devices using global positioning system-supported data collection tools, with recovery of possum carcasses for diagnostic necropsy. Such data provide information subsequently used in predictive epidemiological models to generate a probability of TB freedom.The strategies for managing TB in New Zealand wildlife now operate on four major principles: firstly a target threshold for possum population reduction is defined and set, secondly an objective methodology is applied for assessing whether target reductions have been achieved, thirdly effective control tools for achieving possum population reductions are used, and fourthly the necessary legislative support is in place to ensure compliance. TBfree New Zealand's possum control programme meets these requirements, providing an excellent example of an effective pest and disease control programme.
Development of the New Zealand strategy for local eradication of tuberculosis from wildlife and livestock
Creators: name: G Nugent
name: PG Livingstone
name: N Hancox
name: G Mackereth
name: SA Hutchings
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Computational Biology
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: Science Policy
subject: Inorganic Chemistry
subject: FOS: Chemical sciences
subject: Cancer
subject: Biological Sciences
subject: Ecology
subject: FOS: Biological sciences
subject: Biotechnology
subject: Medicine

description: We describe the progressive development of New Zealand's national strategy for control of tuberculosis (TB) in its agricultural sector over the last four decades. The strategy is globally unique, reflecting the need for effective and co-ordinated management of TB in a wildlife maintenance host, the brushtail possum (Trichosurus vulpecula), in addition to controlling infection in cattle and farmed deer herds. Since the early 1990s, the strategy has been developed by the Animal Health Board (AHB), formed to empower the farming industry to take the leadership role in funding of TB control, policy development and administration.The AHB became the first non-government organisation to develop and gain acceptance by the funders (farming industry and government) of a National Pest Management Strategy (NPMS) under the Biosecurity Act 1993. A key outcome of the NPMS for TB control was the development and inclusion of very challenging objectives that provided direction for management, research and possum control. This paper describes the process whereby the NPMS was revised twice, following achievement of each successive set of strategy objectives within budget. Success was based on firstly, reorganisation of the AHB and its operational systems to achieve increased efficiency; secondly, improved efficiency through contracting possum and disease control, and thirdly research delivering effective and practical applications, while also providing a scientific basis for setting directions for future control strategies. The last revision of the NPMS was implemented in 2011, and included objectives to eradicate Mycobacterium bovis-infected wildlife populations over 2.5 million hectares by 2026. This ambitious objective was adopted only after extensive forecast modelling enabled stakeholders to identify and select the most cost-effective long-term solution for the management of M. bovis-infected possum populations.The accomplishment of New Zealand's TB control programme, in meeting successive sets of demanding NPMS objectives, has seen a 95% decrease in the number of infected cattle and deer herds since they peaked at 1,694 in 1994, and the eradication of TB from infected possum populations from 830,000 hectares. Provided the current level of funding continues, New Zealand is positioned to achieve national eradication of TB well in advance of the 40–50-year timeline forecast 3 years ago.
Entrada y salida del trabajo: comparativa con otros países
Creators: name: Martín Olalla, José María
Publisher: name: Politikon
Year: 2018
Subjects: subject: Econometrics
subject: FOS: Economics and business
subject: Social work
subject: Social sciences
subject: FOS: Social sciences

description: Se describen las frecuencias de ocurrencias de entrada y salida del trabajo en España, Italia, Reino Unido, Dinamarca, Estados Unidos y Canadá. 

Se relacionan estas ocurrencias con el ciclo de luz y oscuridad.

Publicado originalmente en Politikon el 20 de diciembre del año 2018

 

https://politikon.es/2018/12/20/entrada-y-salida-del-trabajo-comparativa-con-otros-paises/#
A Complete Proof of the Collatz Conjecture
Creators: name: Olsson, Ivar
Publisher: name: Zenodo
Year: 2025
Subjects: Collatz Conjecture
Number Theory
Recursive Functions
Discrete Mathematics
Integer Dynamics

description: This preprint presents a complete and elementary proof of the Collatz Conjecture. The work eliminates the possibility of nontrivial cycles by formal derivation of the general cycle formula, and proves convergence to the trivial cycle {1, 2, 4} via a recursive inversion method. The result was independently developed by the author and is intended to serve as a public record of priority and to invite formal verification and commentary.
title: Loop(s) of loneliness: Exploring cognitive and behavioral aspects of (mal)adaptive loneliness
Creators: name: Skoko, Andrej
Publisher: name: Universität Bern
Year: 2024
Subjects: subject: 150 Psychology

description: Loneliness is a profoundly distressing state that stems from an innate need to belong. This pervasive feeling has repeatedly been shown to have associations with detrimental physical and mental health outcomes. One prominent cognitive model delves into the cognitive and behavioral components of loneliness, depicting prolonged loneliness as a vicious self-perpetuating cycle. Despite its widespread acknowledgments in the scientific community and some empirical support, significant gaps remain regarding some of its assumptions. Current interventions addressing loneliness and its cognitive and behavioral aspects have shown promising results. However, the need for low-threshold interventions such as internet-based solutions might be beneficial due to the associated self-stigmatization of lonely individuals.
This dissertation explores the multifaceted phenomenon of loneliness by examining the cognitive underpinnings and behavioral manifestations. Further, it investigates the potential of an internet-based cognitive behavioral intervention in mitigating feelings of loneliness. Four studies conducted within this dissertation underscore the complexity of loneliness and highlight the potential of internet-based cognitive behavioral interventions to alleviate it. The dissertation discusses an alternative and more flexible theoretical approach by implementing a self-regulatory perspective on adaptive and maladaptive loneliness. Based on the results and the self-regulatory perspective, future directions are formulated concerning potential longitudinal studies, the assessment of loneliness, and interventions.
CH3F5 Computer Workshop additional 3 - Taxol (taxotere) + microtubules
Creators: name: Marsh, Andrew
Publisher: name: figshare
Year: 2015
Subjects: subject: Computational Chemistry
subject: 60102 Bioinformatics
subject: FOS: Computer and information sciences
subject: Cheminformatics
subject: Cancer
subject: Biophysics

description: Computer workshop on using PDB. Exercise on microtubule polymerization and Taxol plus other drugs which stabilise or destabilise.
Intergenerational Changes in Knee Height Among Maya Mothers and Their Adult Daughters from Merida, Mexico
Creators: name: Azcorra, Hugo
name: Rodriguez, Luis
name: Varela-Silva, Maria Ines
name: Sudip Data Banik
name: Dickinson, Federico
Publisher: name: figshare
Year: 2015
Subjects: subject: Anthropology
subject: FOS: Sociology
subject: Diseases
subject: Ethnic Studies

description: Objectives: To analyze differences in knee height (KH) between adult Maya mothers and daughters in Merida City, Mexico, and determine if these differences are associated with their childhood socioeconomic conditions.
Methods: From September 2011 to January 2014, we measured KH and collected data on childhood conditions (place of birth, type of drinking water, family size, and fathers’ occupation) from a sample of 180 Maya mother-daughter dyads. Mean KH intergenerational difference was calculated and compared for each category of socioeconomic variables and a multiple regression model was used to assess the association between childhood conditions and KH difference.
Results: A relative increase of 1.05 cm (SD52.3 cm) or 0.45 standard deviations (effect size of difference) was
observed in KH between generations. Place of birth was significantly associated with KH. With three other variables
statistically adjusted for, the intergenerational KH difference was 1.5 cm greater when mothers born outside Merida
but daughters born in the city. Piped water consumption by mother-daughter dyads was associated with 1.5 cm of
increase in KH difference compared with dyads who consumed well water (P50.058).
Conclusions: The relative increase in KH between mothers and daughters represents a portion of the expected
change in growth in a group that has experienced few substantial improvements in their living conditions. Some improvements in childhood living conditions resulting from the intergenerational transition from rural to urban environments
seem to be linked to a modest, but statistically significant intergenerational increase in KH among Maya women in Merida. Am. J. Hum. Biol. 00:000–000, 2015.
CH3F5 Computer Workshop additional 4 - beta adrenergic receptor
Creators: name: Marsh, Andrew
Publisher: name: figshare
Year: 2015
Subjects: Computational Chemistry
Computational Biology
Biochemistry
Cheminformatics
Biophysics

description: CH3F5 Bioorgnaic Chemistry Year 3 module. Workshop on molecular interactions and using the PDB: beta adrenergic receptor.
CH3F5 Computer Workshop 1 - chymotrypsin Jmol version
Creators: name: Marsh, Andrew
name: 0000-0002-9721-0375
Publisher: name: figshare
Year: 2015
Subjects: subject: Computational Chemistry
subject: 60102 Bioinformatics
subject: FOS: Computer and information sciences
subject: Cheminformatics
subject: Biochemistry
subject: Biophysics

description: Computer workshop introduction to using the PDB and visualisation using JMol. Example uses chymotrypsin: 5cha.pdb. Updated Jan 2018.
Multi-Scale Brain Parcellator: a BIDS App for the Lausanne Connectome Parcellation
Creators: name: Tourbier, Sebastien
name: Alemán-Gómez, Yasser
name: Griffa, Alessandra
name: Meritxell Bach Cuadra
name: Hagmann, Patric
Publisher: name: F1000 Research Limited
Year: 2020

description: This poster presents the release of the Multi-scale Brain Parcellator, an open-source processing pipeline software that adopts the BIDS and BIDS App standards and implements the 5-scale Lausanne Connectome Parcellation. It is freely available on GitHub at https://github.com/sebastientourbier/multiscalebrainparcellator.