title: ÐÐ½Ð°ÑÐµÐ½Ð¸Ðµ Ð±ÐµÐ»ÐºÐ¾Ð² Ð²Ð½ÑÑÑÐµÐ½Ð½ÐµÐ³Ð¾ ÑÐµÐ»Ð° ÑÐ°Ð³Ð° phiKZ Ð´Ð»Ñ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸ ÐºÐ»ÐµÑÐ¾Ðº-Ñ Ð¾Ð·ÑÐµÐ²
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Importance of phiKZ phage internal body protein for host cell infection
Creators: name: Ð£ÑÐ°ÑÑÑ, ÐÐ½Ð´ÑÐµÐ¹
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³ phikz
pseudomonas aeruginosa
Ð¿ÑÐµÐ²Ð´Ð¾-ÑÐ´ÑÐ¾
Ð²Ð½ÑÑÑÐµÐ½Ð½ÐµÐµ ÑÐµÐ»Ð¾
Ð±ÐµÐ»ÐºÐ¸ Ð²Ð½ÑÑÑÐµÐ½Ð½ÐµÐ³Ð¾ ÑÐµÐ»Ð°
gp89
gp162
phikz bacteriophage
pseudo-nucleus
inner body
inner body proteins

description: ÐÐ¸Ð³Ð°Ð½ÑÑÐºÐ¸Ð¹ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³ phiKZ Ð¸Ð¼ÐµÐµÑ ÑÐ½Ð¸ÐºÐ°Ð»ÑÐ½ÑÐ¹ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼ ÑÐ¿Ð°ÐºÐ¾Ð²ÐºÐ¸ ÐÐÐ Ð² ÑÐ°Ð³Ð¾Ð²Ð¾Ð¹ ÑÐ°ÑÑÐ¸ÑÐµ â ÐÐÐ Ð½Ð°ÐºÑÑÑÐµÐ½Ð¾ Ð½Ð° Ð¿ÑÐ¾Ð´Ð¾Ð»Ð³Ð¾Ð²Ð°ÑÐ¾Ðµ Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð½Ð°Ð·Ð²Ð°Ð½Ð½Ð¾Ðµ Ð²Ð½ÑÑÑÐµÐ½Ð½Ð¸Ð¼ ÑÐµÐ»Ð¾Ð¼. ÐÐ»Ð¾ÑÐ½Ð¾ÑÑÑ ÑÐ¿Ð°ÐºÐ¾Ð²ÐºÐ¸ ÐÐÐ Ð²Ð½ÑÑÑÐ¸ Ð³Ð¾Ð»Ð¾Ð²Ñ ÑÐ°Ð³Ð° Ð¿ÑÐµÐ²ÑÑÐ°ÐµÑ Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÑ ÑÐ¿Ð°ÐºÐ¾Ð²ÐºÐ¸ Ð²Ð½ÑÑÑÐ¸ ÑÑÐºÐ°ÑÐ¸Ð¾ÑÐ¸ÑÐµÑÐºÐ¸Ñ ÐºÐ»ÐµÑÐ¾Ðº. Ð ÑÐ¾Ð´Ðµ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¸ ÑÐ¾Ð·Ð´Ð°Ð½Ñ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ðµ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ð¸, Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÑÑÑÐ¸Ðµ ÑÐ¾Ð±Ð¾Ð¹ Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð´Ñ, ÑÐ¾Ð´ÐµÑÐ¶Ð°ÑÐ¸Ðµ Ð³ÐµÐ½Ñ Ð±ÐµÐ»ÐºÐ¾Ð² Ð²Ð½ÑÑÑÐµÐ½Ð½ÐµÐ³Ð¾ ÑÐµÐ»Ð° (Gp89 Ð»Ð¸Ð±Ð¾ Gp162), ÑÐ°ÑÐ¿Ð¾Ð»Ð¾Ð¶ÐµÐ½Ð½ÑÐµ ÑÑÐ´Ð¾Ð¼ Ñ Ð³ÐµÐ½Ð¾Ð¼ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° mNeonGreen, ÑÑÐ¾ Ð´ÐµÐ»Ð°Ð»Ð¾ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½ÑÐ¼ ÑÐ¸Ð½ÑÐµÐ· Ð±ÐµÐ»ÐºÐ° Ð²Ð½ÑÑÑÐµÐ½Ð½ÐµÐ³Ð¾ ÑÐµÐ»Ð°, ÑÐ»Ð¸ÑÐ¾Ð³Ð¾ Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½ÑÐ¼ Ð±ÐµÐ»ÐºÐ¾Ð¼. ÐÐ»Ð°Ð·Ð¼Ð¸Ð´Ð°Ð¼Ð¸ Ð±ÑÐ»Ð¸ ÑÑÐ°Ð½ÑÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ñ ÐºÐ»ÐµÑÐºÐ¸ ÑÑÐ°Ð¼Ð¼Ð° Ð seudomonas aeruginosa PAO1. ÐÐ»ÐµÑÐºÐ¸ Ñ Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð´Ð°Ð¼Ð¸ Ð±ÑÐ»Ð¸ Ð¸Ð½ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ñ ÑÐ°Ð³Ð¾Ð¼ phiKZ, Ð¿Ð¾ÑÐ»Ðµ ÑÐµÐ³Ð¾ Ð±ÑÐ»Ð° ÑÐ´ÐµÐ»Ð°Ð½Ð° ÑÐµÑÐ¸Ñ ÑÐ½Ð¸Ð¼ÐºÐ¾Ð² Ð½Ð° ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð¼ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ðµ, ÑÑÐ¾ Ð¿Ð¾Ð·Ð²Ð¾Ð»Ð¸Ð»Ð¾ Ð¾Ð¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ Ð¾ÑÐ¾Ð±ÐµÐ½Ð½Ð¾ÑÑÐ¸ Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð±ÐµÐ»ÐºÐ¾Ð² Ð²Ð½ÑÑÑÐµÐ½Ð½ÐµÐ³Ð¾ ÑÐµÐ»Ð° Ð² ÑÐ¾Ð´Ðµ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸. Ð¢Ð°ÐºÐ¶Ðµ Ð±ÑÐ»Ð° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð¿ÑÐ¾Ð²ÐµÑÐºÐ° Ð½Ð° Ð½Ð°Ð»Ð¸ÑÐ¸Ðµ Ð¿ÑÐ¾ÑÐµÐ¾Ð»Ð¸Ð·Ð° ÑÐ»Ð¸ÑÑÑ Ð±ÐµÐ»ÐºÐ¾Ð² Ð²Ð½ÑÑÑÐµÐ½Ð½ÐµÐ³Ð¾ ÑÐµÐ»Ð° Gp89 Ð¸ Gp162 Ð¿ÑÐ¸ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸ PAO1 ÑÐ°Ð³Ð¾Ð¼ phiKZ. ÐÐ»Ñ ÑÑÐ¾Ð³Ð¾ ÐºÐ»ÐµÑÐºÐ¸ ÑÑÐ°Ð¼Ð¼Ð° Ð . aeruginosa PAO1, ÑÐ¾Ð´ÐµÑÐ¶Ð°ÑÐ¸Ðµ ÑÐµÐ»ÐµÐ²ÑÐµ Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð´Ñ, Ð±ÑÐ»Ð¸ Ð¸Ð½ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ñ ÑÐ°Ð³Ð¾Ð¼ phiKZ, Ð¿Ð¾ÑÐ»Ðµ ÑÐµÐ³Ð¾ Ð±ÑÐ» Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½ Ð²ÐµÑÑÐµÑÐ½-Ð±Ð»Ð¾ÑÑÐ¸Ð½Ð³ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð°Ð½ÑÐ¸-ÑÐµÐ» Ðº ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð¼Ñ Ð±ÐµÐ»ÐºÑ mNeonGreen. Ð ÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð²ÐµÑÑÐµÑÐ½-Ð±Ð»Ð¾ÑÑÐ¸Ð½Ð³Ð° Ð½Ðµ Ð¿Ð¾Ð´ÑÐ²ÐµÑÐ´Ð¸Ð»Ð¸ Ð½Ð°Ð»Ð¸ÑÐ¸Ðµ Ð¿ÑÐ¾ÑÐµÐ¾Ð»Ð¸Ð·Ð° Ð±ÐµÐ»ÐºÐ¾Ð² Ð²Ð½ÑÑÑÐµÐ½Ð½ÐµÐ³Ð¾ ÑÐµÐ»Ð° Gp89 Ð¸ Gp162 Ð¿ÑÐ¸ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸ PAO1 ÑÐ°Ð³Ð¾Ð¼ phiKZ. ÐÐ·ÑÑÐµÐ½Ð¸Ðµ Ð¿Ð¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ Ð±ÐµÐ»ÐºÐ¾Ð² Ð²Ð½ÑÑÑÐµÐ½Ð½ÐµÐ³Ð¾ ÑÐµÐ»Ð° ÑÐ°Ð³Ð° Ð¿ÑÐ¸ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸ Ð´Ð°ÐµÑ Ð½Ð¾Ð²ÑÐµ ÑÐ²ÐµÐ´ÐµÐ½Ð¸Ñ Ð¾Ð± Ð¾ÑÐ¾Ð±ÐµÐ½Ð½Ð¾ÑÑÑÑ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð° Ð·Ð°ÑÐ°Ð¶ÐµÐ½Ð¸Ñ ÐºÐ»ÐµÑÐ¾Ðº ÑÐ°Ð³Ð¾Ð¼ phiKZ. ÐÐ°Ð½Ð½ÑÐµ Ð·Ð½Ð°Ð½Ð¸Ñ Ð¼Ð¾Ð³ÑÑ Ð¿Ð¾Ð·Ð²Ð¾Ð»Ð¸ÑÑ ÑÐ°ÑÑÐ¸ÑÐ¸ÑÑ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»ÑÐ½ÑÐµ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾ÑÑÐ¸ ÑÐ°Ð³Ð¾Ð²Ð¾Ð¹ ÑÐµÑÐ°Ð¿Ð¸Ð¸.
description: This work is devoted to the study of the localization of the innner body proteins of the phiKZ bacteriophage during Pseudomonas aeruginosa infection. For the study, plasmids with the inner body proteins genes (Gp89 or Gp162), located next to the gene for the fluorescent protein mNeonGreen were created to synthesize the innner body proteins fused with the fluorescent protein. Cells of the P. aeruginosa strain PAO1were transformed with the resulted plasmids. Cells with the plasmids were infected with the phiKZ phage, after which a series of images were taken under a fluorescence microscope to determine the peculiarities of the localization of proteins of the inner body during the infection. It was also tested for the presence of proteolysis of the fusion proteins Gp89 and Gp162 during PAO1 infection with the phiKZ phage. For this, cells of the Pseudomonas aeruginosa PAO1 strain containing the target plasmids were infected with the phiKZ phage, after which western-blotting was performed using antibodies to the mNeonGreen fluorescent protein. Western-blotting results did not confirm the presence of proteolysis of the inner body proteins Gp89 and Gp162 during PAO1 infection with the phiKZ phage. The our study results provide new information about the characteristic of the infection mechanism of the phiKZ phage; this knowledge can expand the potential of phage therapy.