Cystobasidium raffinophilum Q. M. Wang, F. Y. Bai & A. H. Li 2020, sp. nov.
Creators: name: Li, A. - H.
name: Yuan, F. - X.
name: Groenewald, M.
name: Bensch, K.
name: Yurkov, A. M.
name: Li, K.
name: Han, P. - J.
name: Guo, L. - D.
name: Aime, M. C.
name: Sampaio, J. P.
name: Jindamorakot, S.
name: Turchetti, B.
name: Inacio, J.
name: Fungsin, B.
name: Wang, Q. - M.
name: Bai, F. - Y.
Publisher: name: Zenodo
Year: 2020
Subjects: Biodiversity
Taxonomy
Fungi
Basidiomycota
Cystobasidiomycetes
Cystobasidiales
Cystobasidiaceae
Cystobasidium
Cystobasidium raffinophilum

description: Cystobasidium raffinophilum Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828822. Fig. 15M.   
 Etymology: the specific epithet raffinophilum refers to the ability to assimilate raffinose.   
 Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ellipsoidal, 3.1–5.0 × 4.5 –6.8 μm and single, budding is polar (Fig. 15M), a sediment is present. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is orange-pink, mucoid,smooth and glistening.The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.   
 Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose (delayed), sucrose, cellobiose, trehalose, raffinose, melezitose, inulin (weak), D-xylose, Larabinose, D-arabinose, D-ribose (delayed and weak), ethanol, glycerol, ribitol (delayed and weak), galactitol, D-mannitol, succinate (weak) and citrate (weak) are assimilated as sole carbon sources. L-sorbose, maltose, lactose, melibiose, soluble starch, Lrhamnose, D-glucosamine, N-Acetyl-D-glucosamine, methanol, erythritol, D-glucitol, Methyl-α- D-glucoside, salicin, DL-lactate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate and potassium nitrate are assimilated as sole nitrogen sources. Sodium nitrite, L-lysine, ethylamine hydrochloride and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 26–27 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.   
 Physiologically, Cy. raffinophilum differs from its closely related species Cy. fimetarium in its inability to assimilate lactose, salicin, DL-lactate and its ability to assimilate galactose, raffi- nose, melezitose, galactitol and potassium nitrate (Table S1.26).   
 Typus: China, Yecheng county, Xinjiang province, obtained from soil, Jul. 2007, Q.-M. Wang (holotype CGMCC 2.3822 T preserved in a metabolically inactive state, ex-type CBS 15509 = 141.4).
description: Published as part of Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, pp. 17-140 in Studies In Mycology 96 on page 116, DOI: 10.1016/j.simyco.2020.01.002