Additional file 1 of Antibiofilm activity of biosynthesized silver and copper nanoparticles using Streptomyces S29
Creators: name: Elshaer, Soha
name: Shaaban, Mona I.
Publisher: name: figshare
Year: 2024
Subjects: subject: Microbiology
subject: FOS: Biological sciences

description: Additional file 1: Table S1. Primer sets used for RT-PCR. Table S2. UV/Vis spectroscopy data of the cell-free supernatant of Streptomyces isolate S29 either untreated or treated with AgNO3 and CuSO4 (5 mM each), in a wavelength range of 300–1000 nm. Figure S1: Optimization of NPs through (a) different concentrations (3 and 5 mM) of CuSO4, (b) different salts of CuSO4 and CuCL2 at fixed concentration (5 mM) with particle size and zeta potential of CuCL2 and different incubation contact time (24 h and 48 h) of (d) Ag-NPs and (e) Cu-NPs. Figure S2: Particle size distribution of (a) Ag-NPs, and (b) Cu-NPs and zeta potentials of biosynthesized (c) Ag-NPs, and (d) Cu-NPs. Figure S3; Effect of different concentration of Ag-NPs and Cu-NPs on the initial attachment and biofilm formation; a) untreated biofilm of A. baumannii ATCC 19606, and A. baumannii A11 stained with crystal violet (CV) method and biofilm treated with (b) Ag-NPs (2x, 1x, 0.5x and 0.25x MIC) and (c) Cu-NPs (50% and 25% concentration) stained with CV. d) untreated biofilm of A. baumannii ATCC 19606, and A. baumannii A11 stained with Tri-phenyl tetrazolium chloride (TTC) method and treated biofilm with (e) Ag-NPs (2x, 1x, 0.5x and 0.25x MIC) and (f) Cu-NPs (50% and 25% concentration) stained with TTC. Figure S4; Effect of different concentration of Ag-NPs and Cu-NPs on the initial attachment and biofilm formation; a) untreated biofilm of K. pneumoniae ATCC 51503, and K. pneumoniae K7 stained with crystal violet (CV) method and biofilm treated with (b) Ag-NPs (2x, 1x, 0.5x and 0.25x MIC) and (c) Cu-NPs (50% and 25% concentration) stained with CV. d) untreated biofilm of K. pneumoniae ATCC 51503, and K. pneumoniae K7 stained with Tri-phenyl tetrazolium chloride (TTC) method and treated biofilm with (e) Ag-NPs (2x, 1x, 0.5x and 0.25x MIC) and (f) Cu-NPs (50% and 25% concentration) stained with TTC. Figure S5; Effect of different concentration of Ag-NPs and Cu-NPs on the initial attachment and biofilm formation; a) untreated biofilm of P. aeruginosa PAO1 and P. aeruginosa P2 stained with crystal violet (CV) method and biofilm treated with (b) Ag-NPs (2x, 1x, 0.5x and 0.25x MIC) and (c) Cu-NPs (50% and 25% concentration) stained with CV. d) untreated biofilm of P. aeruginosa PAO1 and P. aeruginosa P2 stained with Tri-phenyl tetrazolium chloride (TTC) method and treated biofilm with (e) Ag-NPs (2x, 1x, 0.5x and 0.25x MIC) and (f) Cu-NPs (50% and 25% concentration) stained with TTC. Figure S6; Effect of different concentration of Ag-NPs and Cu-NPs on mature biofilm; a) untreated biofilm of A. baumannii ATCC 19606, and A. baumannii A11 stained with crystal violet (CV) method and biofilm treated with (b) Ag-NPs (16x, 8x, 4x, 2x, 1x, 0.5x and 0.25x MIC) and (c) Cu-NPs (100%, 50% and 25%) stained with CV method. d) untreated biofilm of A. baumannii ATCC 19606, and A. baumannii A11 stained with Tri-phenyl tetrazolium chloride (TTC) method and treated biofilm with (e) Ag-NPs (16x, 8x, 4x, 2x, 1x, 0.5x and 0.25x MIC) and (f) Cu-NPs (100%, 50% and 25% concentration) stained with TTC. Figure S7; Effect of different concentration of Ag-NPs and Cu-NPs on mature biofilm; a) untreated biofilm of K. pneumoniae ATCC 51503, and K. pneumoniae K7 stained with crystal violet (CV) method and biofilm treated with (b) Ag-NPs (16x, 8x, 4x, 2x, 1x, 0.5x and 0.25x MIC) and (c) Cu-NPs (100%, 50% and 25% concentration) stained with CV. d) untreated biofilm of K. pneumoniae ATCC 51503, and K. pneumoniae K7 stained with Tri-phenyl tetrazolium chloride (TTC) method and treated biofilm with (e) Ag-NPs (16x, 8x, 4x, 2x, 1x, 0.5x and 0.25x MIC) and (f) Cu-NPs (100, 50% and 25 % concentration) stained with TTC. Figure S8; Effect of different concentration of Ag-NPs and Cu-NPs on the mature; a) untreated biofilm of P. aeruginosa PAO1 and P. aeruginosa P2 stained with crystal violet (CV) method and biofilm treated with (b) Ag-NPs (16x, 8x, 4x, 2x, 1x, 0.5x and 0.25x MIC and (c) Cu-NPs (100%, 50% and 25% concentration) stained with CV method. d) untreated biofilm of P. aeruginosa PAO1 and P. aeruginosa P2 stained with Tri-phenyl tetrazolium chloride (TTC) method and treated biofilm with (e) Ag-NPs (16x, 8x, 4x, 2x, 1x, 0.5x and 0.25x MIC) and (f) Cu-NPs (100%, 50% and 25% concentration) stained with TTC.