title: Ð¢ÑÐµÑ Ð¼ÐµÑÐ½ÑÐµ Ð¿Ð¾ÑÐ¸ÑÑÑÐµ ÑÐºÐ°Ð½ÐµÐ¸Ð½Ð¶ÐµÐ½ÐµÑÐ½ÑÐµ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ð¸ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ Ñ Ð¸ÑÐ¾Ð·Ð°Ð½Ð° Ð¸ Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸Ñ Ð³Ð¸Ð´ÑÐ¾ÐºÑÐ¸Ð°Ð¿Ð°ÑÐ¸ÑÐ° Ð´Ð»Ñ ÐºÐ¾ÑÑÐ½Ð¾Ð¹ Ð¿Ð»Ð°ÑÑÐ¸ÐºÐ¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Three-dimensional porous tissue-engineering scaffolds based on chitosan and hydroxyapatite nanoparticles for bone grafting
Creators: name: ÐÐ¾Ð²Ð¾ÑÐ°Ð´, Ð®ÑÐ¸Ð¹
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÐÐ°ÑÑÐ¸ÑÑ (ÑÐµÑ Ð½.)
ÐÐ¸Ð¾ÑÐµÑ Ð½Ð¾Ð»Ð¾Ð³Ð¸Ñ
Ñ Ð¸ÑÐ¾Ð·Ð°Ð½Ð¾Ð²ÑÐµ Ð¼Ð°ÑÑÐ¸ÑÑ
Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸ÑÑ Ð³Ð¸Ð´ÑÐ¾ÐºÑÐ¸Ð°Ð¿Ð°ÑÐ¸ÑÐ°
ÐºÐ¾ÑÑÐ½Ð°Ñ Ð¿Ð»Ð°ÑÑÐ¸ÐºÐ°
Ð»Ð¸Ð¾ÑÐ¸Ð»ÑÐ½Ð°Ñ ÑÑÑÐºÐ°
Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÐµ ÑÑÑÐ¾Ð¼Ð°Ð»ÑÐ½ÑÐµ ÐºÐ»ÐµÑÐºÐ¸
chitosan scaffolds
hydroxyapatite nanoparticlesbone plastic
bone grafting
freeze drying
mesenchymal stem cells

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description: Topic of master's thesis: "Three-dimensional porous tissue-engineering scaffolds based on chitosan and hydroxyapatite nanoparticles for bone grafting". In the course of the work, chitosan matrices with different content of HA particles were obtained. It was found that HA particles are evenly distributed over the surface of the matrices, which allows cells to freely contact with the particles. The mechanical characteristics of the matrices make it possible to use them for implantation in ââbone tissue defects that do not carry a significant load. It has been shown that matrices deposited in alkali have a negative effect on cellsÂ in vitro. During research, it was found that cell apoptosis is associated with the accumulation of alkali in HA particles and its extraction into the culture medium. To solve this problem, a different method of deposition of chitosan matrix samples was chosen. To convert the matrices into a form insoluble in water, the method of heating samples at a temperature of 100â and heating with the addition of beta-glycerophosphate disodium salt (BGFD) at a temperature of 40â was used. The use of these methods makes it possible to obtain chitosan matrices filled with HA nanoparticles that do not have a negative effect on cells. With such methods of processing chitosan matrices, no changes in the structure of the samples were observed. BGDF also does not affect the structure of the matrices, which reduces the temperature at which the matrices are converted into a form insoluble in water. Changing the methods of converting matrices into a form insoluble in water led to an increase in the elastic characteristics of matrices in dry form.