title: ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ ÑÐ¾ÐºÑÐ¸ÑÐ½Ð¾ÑÑÐ¸ Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸Ñ ÑÐµÑÐµÐ±ÑÐ° Ð½Ð° Ð¼Ð¾Ð´ÐµÐ»Ð¸ C.elegans Ñ Ð½Ð°ÑÑÑÐµÐ½Ð½ÑÐ¼ Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¼Ð¾Ð¼ Ð¼ÐµÐ´Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Study of silver nanoparticles toxicity in C. elegans model with impaired copper metabolism
Creators: name: ÐÐµÑÐ¾Ð²Ð°, ÐÐ»ÐµÐºÑÐ°Ð½Ð´ÑÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Ð¡ÐµÑÐµÐ±ÑÐ¾
ÐÐµÐ´Ñ
Ð±Ð¾Ð»ÐµÐ·Ð½Ñ ÐÐ¸Ð»ÑÑÐ¾Ð½Ð°
Wilsonâs disease

description: ÐÐ°Ð½Ð¾ÑÐ°ÑÑÐ¸ÑÑ ÑÐµÑÐµÐ±ÑÐ° (ÐÐ§Ð¡) ÑÐ¸ÑÐ¾ÐºÐ¾ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·ÑÑÑÑÑ Ð²Ð¾ Ð¼Ð½Ð¾Ð³Ð¸Ñ ÑÑÐµÑÐ°Ñ Ð¿ÑÐ¾Ð¼ÑÑÐ»ÐµÐ½Ð½Ð¾ÑÑÐ¸, Ð¾Ð´Ð½Ð°ÐºÐ¾, Ð¸Ñ Ð±ÐµÐ·Ð¾Ð¿Ð°ÑÐ½Ð¾ÑÑÑ Ð´Ð»Ñ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð¿Ð¾ÐºÐ° Ð½Ðµ Ð´Ð¾ÐºÐ°Ð·Ð°Ð½Ð°. ÐÐ§Ð¡ Ð¿ÑÐ¾Ð´ÑÑÐ¸ÑÑÑÑ Ð¸Ð¾Ð½Ñ Ag(I), ÐºÐ¾ÑÐ¾ÑÑÐµ Ð¸Ð·Ð¾ÑÐ»ÐµÐºÑÑÐ¾Ð½Ð½Ñ Ð¸Ð¾Ð½Ð°Ð¼ Ð¼ÐµÐ´Ð¸ Cu(I) Ð¸ Ð¼Ð¾Ð³ÑÑ Ð½Ð°ÑÑÑÐ°ÑÑ Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¼ Ð¼ÐµÐ´Ð¸, Ð²ÑÑÑÐ°Ð¸Ð²Ð°ÑÑÑ Ð² ÐµÐµ Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸ÑÐµÑÐºÐ¸Ðµ Ð¿ÑÑÐ¸. ÐÐµÐ´Ñ â Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼ÑÐ¹ Ð¼Ð¸ÐºÑÐ¾ÑÐ»ÐµÐ¼ÐµÐ½Ñ Ð´Ð»Ñ Ð²ÑÐµÑ Ð¶Ð¸Ð²ÑÑ Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð¾Ð², Ð¿Ð¾ÑÑÐ¾Ð¼Ñ Ñ Ð½Ð°ÑÑÑÐµÐ½Ð¸ÐµÐ¼ ÐµÐµ Ð³Ð¾Ð¼ÐµÐ¾ÑÑÐ°Ð·Ð° Ð°ÑÑÐ¾ÑÐ¸Ð¸ÑÐ¾Ð²Ð°Ð½ ÑÑÐ´ Ð¼ÐµÐ´Ñ-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼ÑÑ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ð¹. ÐÐ°Ð¿ÑÐ¸Ð¼ÐµÑ, Ð±Ð¾Ð»ÐµÐ·Ð½Ñ ÐÐ¸Ð»ÑÑÐ¾Ð½Ð° (ÐÐ), Ð¿ÑÐ¸ ÐºÐ¾ÑÐ¾ÑÐ¾Ð¹ Ð¼ÐµÐ´Ñ Ð³Ð¸Ð¿ÐµÑÐ°ÐºÐºÑÐ¼ÑÐ»Ð¸ÑÑÐµÑÑÑ Ð² Ð¿ÐµÑÐµÐ½Ð¸ Ð¸ Ð¼Ð¾Ð·Ð³Ðµ, Ð²Ð¾Ð·Ð½Ð¸ÐºÐ°ÐµÑ Ð¸Ð·-Ð·Ð° Ð¼ÑÑÐ°ÑÐ¸Ð¹ Ð² Ð¼ÐµÐ´Ñ-ÑÑÐ°Ð½ÑÐ¿Ð¾ÑÑÐ½Ð¾Ð¹ ÐÐ¢Ð¤Ð°Ð·Ðµ ÐÐ¢Ð 7Ð, Ð² ÐµÐ²ÑÐ¾Ð¿ÐµÐ¹ÑÐºÐ¾Ð¹ Ð¿Ð¾Ð¿ÑÐ»ÑÑÐ¸Ð¸ ÑÐ°ÑÐµ Ð²ÑÐµÐ³Ð¾ Ð²ÑÑÑÐµÑÐ°ÐµÑÑÑ Ð¼ÑÑÐ°ÑÐ¸Ñ H1069Q. ÐÐ»Ñ Ð¿Ð°ÑÐ¸ÐµÐ½ÑÐ¾Ð² Ñ ÐÐ, ÑÐµÐ¹ Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¼ Ð¼ÐµÐ´Ð¸ ÑÐ¶Ðµ Ð½Ð°ÑÑÑÐµÐ½, ÐºÐ¾Ð½ÑÐ°ÐºÑ Ñ ÐÐ§Ð¡ Ð¼Ð¾Ð¶ÐµÑ Ð½ÐµÑÑÐ¸ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»ÑÐ½ÑÑ ÑÐ³ÑÐ¾Ð·Ñ ÑÑÑÐ´ÑÐµÐ½Ð¸Ñ ÑÐµÑÐµÐ½Ð¸Ñ Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸. Ð Ð½Ð°ÑÑÐ¾ÑÑÐµÐ¹ ÑÐ°Ð±Ð¾ÑÐµ Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ Ð½ÐµÐ¼Ð°ÑÐ¾Ð´Ñ C. elegans ÑÑÐ°Ð¼Ð¼Ð¾Ð² Ð´Ð¸ÐºÐ¾Ð³Ð¾ (N2) Ð¸ Ð¼ÑÑÐ°Ð½ÑÐ½Ð¾Ð³Ð¾ (H828Q) ÑÐ¸Ð¿Ð°. ÐÑÑÐ°ÑÐ¸Ñ H828Q Ð² Ð±ÐµÐ»ÐºÐµ CUA-1C. elegans (Ð³Ð¾Ð¼Ð¾Ð»Ð¾Ð³ ÐÐ¢Ð 7Ð) Ð°Ð½Ð°Ð»Ð¾Ð³Ð¸ÑÐ½Ð° Ð¼ÑÑÐ°ÑÐ¸Ð¸ H1069 Ð² ÐÐ¢Ð 7Ð, Ð¿Ð¾ÑÑÐ¾Ð¼Ñ Ð½ÐµÐ¼Ð°ÑÐ¾Ð´Ñ ÑÑÐ°Ð¼Ð¼Ð° H828Q Ð¿ÑÐ¾ÑÐ²Ð»ÑÑÑ ÑÐµÐ½Ð¾ÑÐ¸Ð¿, ÑÑÐ¾Ð¶Ð¸Ð¹ Ñ ÑÐµÐ½Ð¾ÑÐ¸Ð¿Ð¾Ð¼ Ð»ÑÐ´ÐµÐ¹, ÑÑÑÐ°Ð´Ð°ÑÑÐ¸Ñ ÐÐ. Ð¦ÐµÐ»Ñ Ð½Ð°ÑÑÐ¾ÑÑÐµÐ¹ ÑÐ°Ð±Ð¾ÑÑ â Ð¾ÑÐµÐ½Ð¸ÑÑ ÑÐ¾ÐºÑÐ¸ÑÐ½Ð¾ÑÑÑ ÐÐ§Ð¡ Ð´Ð»Ñ C. elegans ÑÑÐ°Ð¼Ð¼Ð¾Ð² N2 Ð¸ H828Q. ÐÐµÐ¼Ð°ÑÐ¾Ð´ Ð¸Ð½ÐºÑÐ±Ð¸ÑÐ¾Ð²Ð°Ð»Ð¸ Ñ ÐÐ§Ð¡ (0 â 2 Ð¼ÐºÐ³/Ð¼Ð») Ð¸ ÑÑÐ°Ð²Ð½Ð¸Ð²Ð°Ð»Ð¸ ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÑ Ð´Ð²ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð² Ð¼ÐµÐ¶Ð´Ñ ÑÐ¾Ð±Ð¾Ð¹. ÐÐ¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ ÐÐ§Ð¡ ÑÐ½Ð¸Ð¶Ð°ÑÑ Ð¿Ð¾Ð´Ð²Ð¸Ð¶Ð½Ð¾ÑÑÑ, ÑÐºÐ¾ÑÐ¾ÑÑÑ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ñ Ð¸ Ð¿ÑÐ¾Ð´Ð¾Ð»Ð¶Ð¸ÑÐµÐ»ÑÐ½Ð¾ÑÑÑ Ð¶Ð¸Ð·Ð½Ð¸ Ð¼ÑÑÐ°Ð½ÑÐ½Ð¾Ð³Ð¾ ÑÑÐ°Ð¼Ð¼Ð° Ð·Ð½Ð°ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾ ÑÐ¸Ð»ÑÐ½ÐµÐµ, ÑÐµÐ¼ Ð´Ð¸ÐºÐ¾Ð³Ð¾, Ð° ÑÐ°ÐºÐ¶Ðµ ÑÑÐ°Ð¼Ð¼ H828Q Ð½Ð°ÐºÐ°Ð¿Ð»Ð¸Ð²Ð°ÐµÑ Ð±Ð¾Ð»ÑÑÐµ Ag, Ð¿Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ ÑÐ¾ ÑÑÐ°Ð¼Ð¼Ð¾Ð¼ N2. Ð¢Ð°ÐºÐ¸Ð¼ Ð¾Ð±ÑÐ°Ð·Ð¾Ð¼, ÐÐ§Ð¡ Ð²ÑÑÐ¾ÐºÐ¾ ÑÐ¾ÐºÑÐ¸ÑÐ½Ñ Ð´Ð»Ñ Ð½ÐµÐ¼Ð°ÑÐ¾Ð´ Ñ Ð½Ð°ÑÑÑÐµÐ½Ð½ÑÐ¼ Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¼Ð¾Ð¼ Ð¼ÐµÐ´Ð¸.
description: Silver nanoparticles (SNPs) are widely used in many industries. However, their safety for humans has not been clearly proven yet. SNPs produce Ag(I) ions, which are isoelectronic to copper ions Cu(I) and can disturb copper metabolism by incorporating into its metabolic pathways. Copper is an essential microelement for all living organisms; therefore, many copper-dependent diseases are associated with copper metabolism disorders. For example, Wilson's disease (WD), which is characterized by copper excessive accumulation in liver and brain, is due to mutations in the copper transport ATPase ATP7B; the H1069Q mutation is the most common in the European population. For patients with WD whose copper metabolism is already impaired, contact with SNPs may have a potential risk of worsening the disease. In this work, the nematode C. elegans of wild (N2) and mutant (H828Q) strains was used as an experimental model. The H828Q mutation in the CUA-1 protein of C. elegans (ATP7B homologue) is similar to the H1069 mutation in ATP7B, so the H828Q nematode strain exhibits a phenotype similar to that of humans with WD. The purpose of this work is to evaluate the toxicity of SNPs to C. elegans strain H828Q. In the work, nematodes were incubated in media with SNP concentrations of 0â2 Î¼g/ml, then various physiological parameters of the two strains were measured and compared to each other. It was shown that SNPs reduce the mobility, development rate, and lifespan of the mutant strain much more strongly than the wild strain, and the H828Q strain accumulates more Ag compared with the N2 strain. Thus, SNPs are highly toxic for nematodes with impaired copper metabolism.