title: Ð¡ÐºÑÐ¸Ð½Ð¸Ð½Ð³ ÑÐ°Ð½Ð½Ð¸Ñ Ð³ÐµÐ½Ð¾Ð² Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð° phiKZ Ð´Ð»Ñ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ ÑÐ¾ÐºÑÐ¸ÑÐ½ÑÑ Ð±ÐµÐ»ÐºÐ¾Ð² Ð¼ÐµÐ¶Ð´Ñ Gp103 Ð¸ Gp113
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Toxicity screening to identify phiKZ anti-microbial early proteins between Gp103 and Gp113
Creators: name: ÐÐ¼Ð¸ÑÑÐ¸ÐµÐ²Ð°, ÐÐ°ÑÑÑÐ¼
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: ÐÐ°ÐºÑÐµÑÐ¸Ð¸
ÐÐµÐ»ÐºÐ¸
ÑÐ°Ð½Ð½Ð¸Ðµ Ð³ÐµÐ½Ñ
Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³ phiKZ
Ð°Ð½ÑÐ±Ð¸Ð¾ÑÐ¸ÐºÐ¾ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾ÑÑÑ
early genes
phiKZ bacteriophage
antibiotic resistance

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð´Ð²ÑÑ ÑÐ°Ð½Ð½Ð¸Ñ Ð³ÐµÐ½Ð¾Ð² Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð° phiKZ Ñ ÑÐµÐ»ÑÑ Ð¿Ð¾Ð¸ÑÐºÐ° Ð±ÐµÐ»ÐºÐ¾Ð², Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÑÑÑÐ¸Ñ ÑÐ¾ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð±Ð°ÐºÑÐµÑÐ¸Ð¹. Ð ÑÐ¾Ð´Ðµ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð° Ð¾ÑÑÑÐµÑÑÐ²Ð»ÐµÐ½Ð° ÑÐ±Ð¾ÑÐºÐ° ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¾Ð½Ð½ÑÑ Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð´, ÐºÐ¾Ð´Ð¸ÑÑÑÑÐ¸Ñ Ð±ÐµÐ»ÐºÐ¸, ÑÐ»Ð¸ÑÑÐµ Ñ Ð°ÑÑÐ¸Ð½Ð½ÑÐ¼Ð¸ Ð¿Ð¾Ð»Ð¸Ð¿ÐµÐ¿ÑÐ¸Ð´Ð°Ð¼Ð¸. ÐÑÐ»Ð¾ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¾ ÑÐµÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² gp103 Ð¸ gp113 Ð½Ð° Ð¶Ð¸Ð·Ð½ÐµÐ´ÐµÑÑÐµÐ»ÑÐ½Ð¾ÑÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹. ÐÐµÑÐ²ÑÐ¹ ÑÑÐ°Ð¿ ÑÐµÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð·Ð°ÐºÐ»ÑÑÐ°Ð»ÑÑ Ð² Ð²ÑÑÐµÐ²Ð°Ð½Ð¸Ð¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹, ÑÐ¾Ð´ÐµÑÐ¶Ð°ÑÐ¸Ñ Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð´Ñ Ñ Ð³ÐµÐ½Ð°Ð¼Ð¸, ÐºÐ¾Ð´Ð¸ÑÑÑÑÐ¸Ð¼Ð¸ Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼ÑÐµ Ð±ÐµÐ»ÐºÐ¸, Ð² Ð¿ÑÐ¸ÑÑÑÑÑÐ²Ð¸Ð¸ Ð¸ Ð¾ÑÑÑÑÑÑÐ²Ð¸Ð¸ Ð¸Ð½Ð´ÑÐºÑÐ¾ÑÐ°. ÐÑÐ¾ÑÐ¾Ð¹ ÑÑÐ°Ð¿ ÑÐµÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð·Ð°ÐºÐ»ÑÑÐ°Ð»ÑÑ Ð² Ð°Ð½Ð°Ð»Ð¸Ð·Ðµ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹ Ð¾Ð¿ÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÐµÐ¹ ÐºÑÐ»ÑÑÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð² Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾ÑÑÐ¸ Ð¾Ñ ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ñ Ð¸Ð½Ð´ÑÐºÑÐ¾ÑÐ° Ð² ÑÑÐµÐ´Ðµ. Ð ÑÐ¾Ð´Ðµ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ¾Ð² Ð±ÑÐ» Ð²ÑÑÐ²Ð»ÐµÐ½ Ð¾Ð´Ð¸Ð½ Ð±ÐµÐ»Ð¾Ðº-ÐºÐ°Ð½Ð´Ð¸Ð´Ð°Ñ, Ð¿ÑÐ¾ÑÐ²Ð»ÑÑÑÐ¸Ð¹ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÑÑÑÑÑ ÑÐ¾ÑÑ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð² Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ð¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ P. aeruginosa Ð¸ E. coli. ÐÑÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¼ÐµÑÐ°Ð»Ð»Ð¾-Ð¾Ð±Ð¼ÐµÐ½Ð½Ð¾Ð¹ ÑÑÐ¾Ð¼Ð°ÑÐ¾Ð³ÑÐ°ÑÐ¸Ð¸ Ð¿Ð¾Ð·Ð²Ð¾Ð»Ð¸Ð»Ð¾ Ð¿Ð¾Ð´ÑÐ²ÐµÑÐ´Ð¸ÑÑ Ð½Ð°Ð»Ð¸ÑÐ¸Ðµ Ð±ÐµÐ»ÐºÐ° Gp103 Ð²Ð½ÑÑÑÐ¸ ÐºÐ»ÐµÑÐ¾Ðº. ÐÐ»Ð°Ð³Ð¾Ð´Ð°ÑÑ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð½ÑÐ¼ Ð±Ð°ÐºÑÐµÑÐ¸ÑÐ¸Ð´Ð½ÑÐ¼ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð°Ð¼ Ð±ÐµÐ»ÐºÐ° Gp103, Ð´Ð°Ð½Ð½Ð¾Ðµ Ð¾ÑÐºÑÑÑÐ¸Ðµ Ð¸Ð¼ÐµÐµÑ Ð±Ð¾Ð»ÑÑÐ¾Ð¹ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð» Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð² Ð»ÐµÑÐµÐ½Ð¸Ð¸ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¹, Ð²ÑÐ·Ð²Ð°Ð½Ð½ÑÑ Ð³ÑÐ°Ð¼Ð¾ÑÑÐ¸ÑÐ°ÑÐµÐ»ÑÐ½ÑÐ¼Ð¸ P. aeruginosa Ð¸ E. coli.
description: The given work is devoted to the study of two early bacteriophage genes phiKZ in order to find proteins that inhibit the growth of bacterial cells. In the course of the work, was carried out the assembly of expression plasmids encoding proteins fused with affine polypeptides. The effect of gp103 and gp113 gene expression on the vital activity of bacteria was tested. The first stage of testing consisted in seeding bacteria containing plasmids with genes encoding the studied proteins in the presence and absence of an inductor. The second stage of testing consisted in the analysis of changes in the optical densities of cell cultures depending on the content of the inductor in the medium. During the experiments, one candidate protein was identified that exhibits growth-inhibiting activity against P. aeruginosa and E. coli bacteria. Isolation by metal-exchange chromatography made it possible to confirm the presence of Gp103 protein inside cells. Due to the discovered bactericidal properties of the Gp103 protein, this discovery has great potential for use in the treatment of infections caused by gram-negative P. aeruginosa and E. coli.