title: ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð·Ð°ÑÐ¸ÑÐ½ÑÑ ÑÐ²Ð¾Ð¹ÑÑÐ² Bacillus subtilis Ð¿ÑÐ¸ Ð²ÑÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ð¸ ÐºÑÐ»ÑÑÑÑ Tagetes Ð½Ð° Ð·Ð°Ð³ÑÑÐ·Ð½ÐµÐ½Ð½ÑÑ Ð¿Ð¾ÑÐ²Ð°Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Research of the protective properties of Bacillus subtilis at growing Tagetes crops on polluted soils
Creators: name: Ð§Ð¶ÐµÐ½, ÐÐ¸ÐºÑÐ¾ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: ÑÐ¸ÑÐ¾ÑÐµÐ¼ÐµÐ´Ð¸Ð°ÑÐ¸Ñ
ÑÑÐ¶ÐµÐ»ÑÐµ Ð¼ÐµÑÐ°Ð»Ð»Ñ
Ð´ÐµÐºÐ¾ÑÐ°ÑÐ¸Ð²Ð½ÑÐµ ÐºÑÐ»ÑÑÑÑÑ
Ð±Ð°ÑÑ Ð°ÑÑÑ
phytoremediation
heavy metals
ornamental plant crops
Tagetes

description: Ð¦ÐµÐ»ÑÑ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÐµÑÑÑ Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Bacillus subtilis Ð½Ð° ÑÐ¾ÑÑ Ð¸ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ðµ Ð±Ð°ÑÑÐ°ÑÑÐµÐ² (Tagetes), ÑÐ°ÑÑÑÑÐ¸Ñ Ð½Ð° Ð¿Ð¾ÑÐ²Ð°Ñ, Ð·Ð°Ð³ÑÑÐ·Ð½ÐµÐ½Ð½ÑÑ ÑÑÐ¶ÐµÐ»ÑÐ¼Ð¸ Ð¼ÐµÑÐ°Ð»Ð»Ð°Ð¼Ð¸.Â  ÐÐ°Ð´Ð°ÑÐ¸, ÑÐµÑÐ°ÐµÐ¼ÑÐµ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: - Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÑ Ð¿Ð¾ ÑÐµÐ¼Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ; - Ð²ÑÑÐ°Ð´ÐºÐ° Ð±Ð°ÑÑÐ°ÑÑÐµÐ² (Tagetes) Ð² Ð¿Ð¾ÑÐ²Ð¾ÑÐ¼ÐµÑÐ¸ Ñ Ð·Ð°ÑÐ°Ð½ÐµÐµ Ð²Ð²ÐµÐ´ÐµÐ½Ð½Ð¾Ð¹ ÐºÑÐ»ÑÑÑÑÐ¾Ð¹ Bacillus subtilis Ð¸ Ñ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÐ¼Ð¸ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸ÑÐ¼Ð¸ ÑÑÐ¶ÐµÐ»ÑÑ Ð¼ÐµÑÐ°Ð»Ð»Ð¾Ð² (Ð¼ÐµÐ´Ð¸, ÑÐ²Ð¸Ð½ÑÐ° Ð¸ ÑÐ¸Ð½ÐºÐ°); - Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Bacillus subtilis Ð¸ ÑÑÐ¶ÐµÐ»ÑÑ Ð¼ÐµÑÐ°Ð»Ð»Ð¾Ð² (Ð¼ÐµÐ´Ð¸, ÑÐ²Ð¸Ð½ÑÐ° Ð¸ ÑÐ¸Ð½ÐºÐ°) Ð½Ð° ÑÐ¾ÑÑ Ð¸ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ðµ Ð±Ð°ÑÑÐ°ÑÑÐµÐ² Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ð°Ð½Ð¸Ð¸ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð° Ð²ÑÐ¶Ð¸Ð²ÑÐ¸Ñ ÑÐ°ÑÑÐµÐ½Ð¸Ð¹ Ð¸ ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¸Ðµ Ð¼ÐµÑÐ°Ð»Ð»Ð°, Ðº ÐºÐ¾ÑÐ¾ÑÐ¾Ð¼Ñ ÑÐ°ÑÑÐµÐ½Ð¸Ñ Ð¿ÑÐ¾ÑÐ²Ð»ÑÑÑ Ð½Ð°Ð¸Ð±Ð¾Ð»ÑÑÑÑ ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾ÑÑÑ; - Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Bacillus subtilis Ð½Ð° Ð²Ð½ÐµÑÐ½Ð¸Ð¹ Ð²Ð¸Ð´ Ð¸ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÑ (Ð²ÐµÑ Ð¸ Ð²ÑÑÐ¾ÑÑ) Ð±Ð°ÑÑÐ°ÑÑÐµÐ², Ð²ÑÑÐ°ÑÐµÐ½Ð½ÑÑ Ð´Ð¾ Ð¿Ð¾Ð»Ð½Ð¾ÑÐµÐ½Ð½Ð¾Ð³Ð¾ ÑÐ²ÐµÑÑÑÐµÐ³Ð¾ ÑÐ°ÑÑÐµÐ½Ð¸Ñ Ð½Ð° Ð¿Ð¾ÑÐ²Ð¾ÑÐ¼ÐµÑÑÑ Ñ Ð¼ÐµÐ´ÑÑ Ð² ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸ÑÑ; - Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ Ð¼Ð¸ÐºÑÐ¾ÑÐ»Ð¾ÑÑ Ð¿ÑÐ¸ÐºÐ¾ÑÐ½ÐµÐ²Ð¾Ð¹ Ð¾Ð±Ð»Ð°ÑÑÐ¸ Ð±Ð°ÑÑÐ°ÑÑÐµÐ², Ð²ÑÑÐ°ÑÐµÐ½Ð½ÑÑ Ð½Ð° Ð¿Ð¾ÑÐ²Ð°Ñ, Ð·Ð°Ð³ÑÑÐ·Ð½ÐµÐ½Ð½ÑÑ Ð¼ÐµÐ´ÑÑ Ð¸ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾ÑÑÐ¸ ÐºÑÐ»ÑÑÑÑÑ Bacillus subtilis s21 Ðº Ð¼ÐµÐ´Ð¸. ÐÐ±ÑÐµÐºÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: Ð´ÐµÐºÐ¾ÑÐ°ÑÐ¸Ð²Ð½ÑÐµ ÑÐ°ÑÑÐµÐ½Ð¸Ñ Ð¸Ð· ÑÐ¾Ð´Ð° Ð±Ð°ÑÑÐ°ÑÑÑ (Tagetes), ÐºÑÐ»ÑÑÑÑÐ° Bacillus subtilis s21. ÐÑÐ¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ð¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ Ð¾Ð±ÑÐ°Ð·ÑÑ Ð¿Ð¾ÑÐ²Ð¾ÑÐ¼ÐµÑÐµÐ¹ c ÑÑÐ¶ÐµÐ»ÑÐ¼Ð¸Â  Ð¼ÐµÑÐ°Ð»Ð»Ð°Ð¼Ð¸ (Ð¼ÐµÐ´ÑÑ, ÑÐ²Ð¸Ð½ÑÐ¾Ð¼ Ð¸ ÑÐ¸Ð½ÐºÐ¾Ð¼) Ð² Ð·Ð°Ð´Ð°Ð½Ð½ÑÑ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸ÑÑ. ÐÑÐ°ÐºÑÐ¸ÑÐµÑÐºÐ°Ñ ÑÐ°ÑÑÑ ÑÐ°Ð±Ð¾ÑÑ Ð²ÑÐ¿Ð¾Ð»Ð½ÑÐ»Ð°ÑÑ Ð½Ð° Ð±Ð°Ð·Ðµ ÐÐÐ¦ Â«ÐÐ¸Ð¼Ð½Ð¸Ð¹ ÑÐ°Ð´Â» ÐÐÐ£ Ð¸Ð¼. ÐÑÑÐºÐ¸Ð½Ð° Ð¸ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ Ð¤ÐÐÐÐ£ ÐÐÐÐÐ¡Ð¥Ð. Ð ÑÐ¾Ð´Ðµ ÑÐ°Ð±Ð¾ÑÑ ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¾, ÑÑÐ¾ ÑÑÐ°Ð¼Ð¼ Bacillus subtilis s21 ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸Ð·ÑÐµÑÑÑ Ð½Ð¸Ð·ÐºÐ¾Ð¹ ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾ÑÑÑÑ Ðº Ð²ÑÑÐ¾ÐºÐ¸Ð¼ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸ÑÐ¼ ÑÑÐ¶ÐµÐ»ÑÑ Ð¼ÐµÑÐ°Ð»Ð»Ð¾Ð², Ð½Ð¾ Ð¿Ð¾Ð»Ð¾Ð¶Ð¸ÑÐµÐ»ÑÐ½Ð¾ Ð²Ð»Ð¸ÑÐµÑ Ð½Ð° Ð²ÑÐ¶Ð¸Ð²Ð°ÐµÐ¼Ð¾ÑÑÑ Ð±Ð°ÑÑÐ°ÑÑÐµÐ² (Tagetes) Ð½Ð° Ð¿Ð¾ÑÐ²Ð°Ñ Ñ ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸ÐµÐ¼ Ð¼ÐµÐ´Ð¸ Ð¸ ÑÐ¸Ð½ÐºÐ°. ÐÑÐ»ÑÑÑÑÐ° Bacillus subtilis Ð² ÑÐ¾ÑÑÐ°Ð²Ðµ Ð¿Ð¾ÑÐ²Ð¾ÑÐ¼ÐµÑÐ¸ ÑÐ¿Ð¾ÑÐ¾Ð±ÑÑÐ²ÑÐµÑ ÑÐ»ÑÑÑÐµÐ½Ð¸Ñ Ð²Ð½ÐµÑÐ½ÐµÐ³Ð¾ Ð²Ð¸Ð´Ð° Ð±Ð°ÑÑÐ°ÑÑÐµÐ² Ð½Ð° ÑÑÐ°Ð´Ð¸Ð¸ ÑÐ²ÐµÑÐµÐ½Ð¸Ñ. ÐÐ½Ð° Ð½Ðµ Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÐµÑ ÑÑÑÐµÑÑÐ²ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð½Ð° Ð²ÑÑÐ¾ÑÑ Ð¸ Ð²ÐµÑ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð±Ð°ÑÑÐ°ÑÑÐµÐ², Ð²ÑÑÐ°ÑÐ¸Ð²Ð°ÐµÐ¼ÑÑ Ð½Ð° Ð¿Ð¾ÑÐ²Ð°Ñ, Ð·Ð°Ð³ÑÑÐ·Ð½ÐµÐ½Ð½ÑÑ Ð¼ÐµÐ´ÑÑ. ÐÐ½ÐµÑÐµÐ½Ð¸Ðµ ÐºÑÐ»ÑÑÑÑÑ Bacillus subtilis s21 ÑÐ²ÐµÐ»Ð¸ÑÐ¸Ð²Ð°ÐµÑ Ð¾Ð±ÑÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÑ Ð¾Ð±ÑÐµÐ¼ÐµÐ½ÐµÐ½Ð½Ð¾ÑÑÑ Ð¿ÑÐ¸ÐºÐ¾ÑÐ½ÐµÐ²Ð¾Ð¹ Ð·Ð¾Ð½Ñ Ð±Ð°ÑÑÐ°ÑÑÐµÐ². ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð¼Ð¾Ð³ÑÑ Ð±ÑÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð¾ÑÐ½Ð¾Ð²Ñ Ð´Ð»Ñ Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐ¸Ñ, Ð±Ð¾Ð»ÐµÐµ Ð¼Ð°ÑÑÑÐ°Ð±Ð½ÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ Ð² Ð¾Ð±Ð»Ð°ÑÑÐ¸ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð±Ð°ÑÑÐ°ÑÑÐµÐ² (Tagetes), Ð²ÑÑÐ°ÑÐ¸Ð²Ð°ÐµÐ¼ÑÑ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¼Ð¸ÐºÑÐ¾Ð±Ð½ÑÑ Ð±Ð¸Ð¾Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð², Ð´Ð»Ñ Ð¾ÑÐ¸ÑÑÐºÐ¸ Ð·Ð°Ð³ÑÑÐ·Ð½ÐµÐ½Ð½ÑÑ Ð¿Ð¾ÑÐ² Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ Ð³Ð¾ÑÐ¾Ð´Ð°. ÐÑÐ¸ Ð½Ð°Ð¿Ð¸ÑÐ°Ð½Ð¸Ð¸ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±Ð·Ð¾ÑÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ: eLIBRARY.RU, Scopus. Ð¡ÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÑÑ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»Ð¸ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿Ð°ÐºÐµÑÐ° Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼: Microsoft Excel, Microsoft Word.
description: The aim of the work is to study the effect of Bacillus subtilis on the growth and development of marigolds (Tagetes) growing on soils contaminated with heavy metals. Tasks to be solved during the research: - study of the literature on the research topic; - planting of marigolds (Tagetes) in soil mixtures with a pre-introduced culture of Bacillus subtilis and with different concentrations of heavy metals (copper, lead, and zinc); - investigation of the effect of Bacillus subtilis and heavy metals (copper, lead, and zinc) on the growth and development of marigolds based on the number of surviving plants and the establishment of the metal to which plants show the greatest resistance; - determination of the effect of Bacillus subtilis on the appearance and morphological parameters (weight and height) of marigolds grown to a full-fledged flowering plant on soil mixtures with copper in various concentrations; - study of the microflora of the root region of marigolds grown on soils contaminated with copper and determination of the resistance of Bacillus subtilis s21 culture to copper. Objects of research: ornamental plants from the genus marigolds (Tagetes), culture of Bacillus subtilis s21. During the research, samples of soil mixtures with heavy metals (copper, lead, and zinc) in specified concentrations were used. The practical part of the work was carried out on the basis of the Pushkin Leningrad State University and the Laboratory of All-Russia Research Institute for AgriculturalÂ  Microbiology. During the work, it was found that the Bacillus subtilis s21 strain is characterized by low resistance to high concentrations of heavy metals but has a positive effect on the survival of marigolds (Tagetes) on soils containing copper and zinc. The culture of Bacillus subtilis in the composition of the soil mixture helps to improve the appearance of marigolds at the flowering stage. It does not significantly affect the height and weight of the biomass of marigolds grown on soils contaminated with copper. The introduction of Bacillus subtilis s21 culture increases the total bacterial contamination of the root zone of marigolds. The results obtained can be used as a basis for further, more extensive research in the field of marigold (Tagetes) grown using microbial biologics to clean polluted soils in urban conditions. When writing the literature review, the following databases were used: eLIBRARY.RU, Scopus. Statistical processing of experimental data was carried out using the software package: Microsoft Excel, Microsoft Word.