cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Cross-reactive monoclonal antibodies to multiple HIV-1 subtype and SIVcpz envelope glycoproteins
Creators: name: Moody, M. Anthony
name: Cohen, Myron S.
name: Gao, Feng
name: Hora, Bhavna
name: Fiscus, Susan A.
name: Korber, Bette T.
name: Haynes, Barton F.
name: Liao, Hua-xin
name: Hahn, Beatrice H.
name: Lomas, Woodrow E.
name: Alam, S. Munir
name: Parks, Robert J.
name: Ogburn, Damon F.
name: Maino, Vernon C.
name: Hohm, Julie E.
name: Xia, Shimao
name: Park, Emily
name: Scearce, Richard M.
name: Tomaras, Georgia D.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2009

description: The extraordinarily high level of genetic variation of HIV-1 env genes poses a challenge to obtain antibodies that cross-react with multiple subtype Env glycoproteins. To determine if cross-reactive monoclonal antibodies (mAbs) to highly conserved epitopes in HIV-1 envelope glycoproteins can be induced, we immunized mice with wild-type or consensus HIV-1 Env proteins and characterized a panel of ten mAbs that reacted with varying breadth to subtypes A, B, C, D, F, G, CRF01_AE and a highly divergent SIVcpzUS Env proteins by ELISA and Western blot analysis. Two mAbs (3B3 and 16H3) cross–reacted with all tested Env proteins, including SIVcpzUS Env. Surface plasmon resonance analyses showed both 3B3 and 16H3 bound Env proteins with high affinity. However, neither neutralized primary HIV-1 pseudoviruses. These data indicate that broadly-reactive non-neutralizing monoclonal antibodies can be elicited, but that the conserved epitopes that they recognize are not present on functional virion trimers. Nonetheless, such mAbs represent valuable reagents to study the biochemistry and structural biology of Env protein oligomers.
Dengue virus neutralization by human immune sera: Role of envelope protein domain III-reactive antibody
Creators: name: Kraus, Annette A.
name: de Silva, Aravinda M.
name: Wahala, W.M.P.B.
name: Accavitti-Loper, Mary Ann
name: Haymore, Laura B.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2009

description: Dengue viruses (DENV) are the etiological agents of dengue fever (DF) and dengue hemorrhagic fever (DHF). The DENV complex consists of four closely related viruses designated DENV serotypes 1 through-4. Although infection with one serotype induces cross reactive antibody to all 4 serotypes, the long term protective antibody response is restricted to the serotype responsible for infection. Cross-reactive antibodies appear to enhance infection during a second infection with a different serotype. The goal of the present study was to characterize the binding specificity and functional properties of human DENV immune sera. The study focused on domain III of the viral envelope protein (EDIII), as this region has a well characterized epitope that is recognized by strongly neutralizing serotype-specific mouse monoclonal antibodies (Mabs). Our results demonstrate that EDIII-reactive antibodies are present in primary and secondary DENV immune human sera. Human antibodies bound to a serotype specific epitope on EDIII after primary infection and a serotype cross reactive epitope on EDIII after secondary infection. However, EDIII-binding antibodies constituted only a small fraction of the total antibody in immune sera binding to DENV. Studies with complete and EDIII antibody depleted human immune sera demonstrated that EDIII binding antibodies play a minor role in DENV neutralization. We propose that human antibodies directed to other epitopes on the virus are primarily responsible for DENV neutralization. Our results have implications for understanding protective immunity following natural DENV infection and for evaluating DENV vaccines.
Inspección de obras viales en la Dirección de Proyectos Especiales del Ministerio de Obras Públicas
Creators: name: Lopez, Bayron
Publisher: name: figshare
Year: 2022
Subjects: subject: 90502 Construction Engineering
subject: FOS: Civil engineering
subject: 90501 Civil Geotechnical Engineering
subject: 91503 Engineering Practice
subject: FOS: Other engineering and technologies

description: En este trabajo se resumirá la experiencia profesional adquirida por el estudiante en la Dirección de Proyectos Especiales del Ministerio de Obras Públicas, donde tuvo la oportunidad de participar, en diferentes etapas de su práctica, en los equipos de inspección pertenecientes a los proyectos: Carretera Transístmica, tramo Plaza Ágora-Estación San Isidro, Intercambiador Vial (Howard), Tramo: Puente de las Américas-Arraiján, Ampliación 8 carriles Arraiján. Donde además de desempeñar el papel de inspector de campo, también fungió de asistente en labores administrativas, como en revisiones de cuentas de cobro, trámites de adendas y otras labores propias del cargo de Ingeniero Residente para un proyecto del Estado.
A rev1–vpu polymorphism unique to HIV-1 subtype A and C strains impairs envelope glycoprotein expression from rev–vpu–env cassettes and reduces virion infectivity in pseudotyping assays
Creators: name: McPherson, David T.
name: Hahn, Beatrice H.
name: Shaw, Katharina S.
name: Shaw, George M.
name: Swanstrom, Ronald
name: Parrish, Nicholas F.
name: Williamson, Carolyn
name: Salazar-Gonzalez, Jesus F.
name: Keele, Brandon F.
name: Anderson, Jeffrey A.
name: Ping, Li-Hua
name: Kraus, Matthias H.
name: Grayson, Truman
name: Decker, Julie M.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2010

description: Functional studies of HIV-1 envelope glycoproteins (Envs) commonly include the generation of pseudoviruses, which are produced by co-transfection of rev-vpu-env cassettes with an env-deficient provirus. Here, we describe six Env constructs from transmitted/founder HIV-1 that were defective in the pseudotyping assay, although two produced infectious virions when expressed from their cognate proviruses. All of these constructs exhibited an unusual gene arrangement in which the first exon of rev (rev1) and vpu were in the same reading frame without an intervening stop codon. Disruption of the rev1-vpu fusion gene by frameshift mutation, stop codon, or abrogation of the rev initiation codon restored pseudovirion infectivity. Introduction of the fusion gene into wildtype Env cassettes severely compromised their function. The defect was not due to altered env and rev transcription or a dominant negative effect of the expressed fusion protein, but seemed to be caused by inefficient translation at the env initiation codon. Although the rev1-vpu polymorphism affects Env expression only in vitro, it can cause problems in studies requiring Env complementation, such as analyses of co-receptor usage and neutralization properties, since 3% of subtype A, 20% of subtype C and 5% of CRF01_A/E viruses encode the fusion gene. A solution is to eliminate the rev initiation codon when amplifying rev-vpu-env cassettes since this increases Env expression irrespective of the presence of the polymorphism.
True precision limits in quantum metrology
Creators: name: Jarzyna, Marcin
name: Demkowicz-Dobrzanski, Rafal
Publisher: name: arXiv
Year: 2014
Subjects: subject: Quantum Physics (quant-ph)
subject: FOS: Physical sciences

description: We show that quantification of the performance of quantum-enhanced measurement schemes based on the concept of quantum Fisher information yields asymptotically equivalent results as the rigorous Bayesian approach, provided generic uncorrelated noise is present in the setup. At the same time, we show that for the problem of decoherence-free phase estimation this equivalence breaks down and the achievable estimation uncertainty calculated within the Bayesian approach is by a $π$ factor larger than that predicted by the QFI even in the large prior knowledge (small parameter fluctuation) regime, where QFI is conventionally regarded as a reliable figure of merit. We conjecture that the analogous discrepancy is present in arbitrary decoherence-free unitary parameter estimation scheme and propose a general formula for the asymptotically achievable precision limit. We also discuss protocols utilizing states with indefinite number of particles and show that within the Bayesian approach it is legitimate to replace the number of particles with the mean number of particles in the formulas for the asymptotic precision, which as a consequence provides another argument that proposals based on the properties of the QFI of indefinite particle number states leading to sub-Heisenberg precisions are not practically feasible.
description: 20 pages, 4 figures
Epstein–Barr virus BRLF1 inhibits transcription of IRF3 and IRF7 and suppresses induction of interferon-β
Creators: name: Pagano, Joseph S.
name: Liu, Renshui
name: Hahn, Angela M.
name: Bentz, Gretchen L.
name: Shackelford, Julia
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2010

description: Activation of interferon regulatory factors (IRFs) 3 and 7 is essential for the induction of Type I interferons (IFN) and innate antiviral responses, and herpesviruses have evolved mechanisms to evade such responses. We previously reported that Epstein-Barr Virus BZLF1, an immediate-early (IE) protein, inhibits the function of IRF7, but the role of BRLF1, the other IE transactivator, in IRF regulation has not been examined. We now show that BRLF1 expression decreased induction of IFN-β, and reduced expression of IRF3 and IRF7; effects were dependent on N- and C-terminal regions of BRLF1 and its nuclear localization signal. Endogenous IRF3 and IRF7 RNA and protein levels were also decreased during cytolytic EBV infection. Finally, production of IFN-β was decreased during lytic EBV infection and was associated with increased susceptibility to superinfection with Sendai virus. These data suggest a new role for BRLF1 in the ability of to evade host innate immune responses.
Phase diagram of the J1-J2 Heisenberg model on the kagome lattice
Creators: name: Kolley, Fabian
name: Depenbrock, Stefan
name: McCulloch, Ian P.
name: Schollwöck, Ulrich
name: Alba, Vincenzo
Publisher: name: arXiv
Year: 2014
Subjects: subject: Strongly Correlated Electrons (cond-mat.str-el)
subject: Statistical Mechanics (cond-mat.stat-mech)
subject: Quantum Physics (quant-ph)
subject: FOS: Physical sciences

description: We perform an extensive density matrix renormalization group (DMRG) study of the ground-state phase diagram of the spin-1/2 J_1-J_2 Heisenberg model on the kagome lattice. We focus on the region of the phase diagram around the kagome Heisenberg antiferromagnet, i.e., at J_2=0. We investigate the static spin structure factor, the magnetic correlation lengths, and the spin gaps. Our results are consistent with the absence of magnetic order in a narrow region around J_2\approx 0, although strong finite-size effects do not allow us to accurately determine the phase boundaries. This result is in agreement with the presence of an extended spin-liquid region, as it has been proposed recently. Outside the disordered region, we find that for ferromagnetic and antiferromagnetic J_2 the ground state displays signatures of the magnetic order of the \sqrt{3}\times\sqrt{3} and the q=0 type, respectively. Finally, we focus on the structure of the entanglement spectrum (ES) in the q=0 ordered phase. We discuss the importance of the choice of the bipartition on the finite-size structure of the ES.
description: 9 pages, 10 figures. Figure added, minor modifications, as published
Modulation of type I IFN induction by a virulence determinant within the alphavirus nsP1 protein
Creators: name: Heise, Mark T.
name: Morrison, Thomas E.
name: Johnston, Robert E.
name: Shabman, Reed
name: Montgomery, Stephanie A.
name: Suthar, Mehul S.
name: Cruz, Catherine C.
name: Simmons, Jason
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2010

description: Alphaviruses are mosquito-borne viruses that cause serious human and animal diseases. Previous studies demonstrated that a determinant within the nsP1/nsP2 cleavage domain of the virulent Sindbis AR86 virus played a key role in regulating adult mouse virulence without adversely affecting viral replication. Additional characterization of this determinant demonstrated that a virus with the attenuating mutation induced more type I IFN production both in vivo and in vitro. Interestingly, this phenotype was not specific to the Sindbis AR86 virus, as a similar mutation in a distantly related alphavirus, Ross River Virus (RRV), also led to enhanced IFN induction. This effect was independent of virus-induced host shutoff, since IRF-3 phosphorylation, which occurs independently of de novo host transcription/translation, was induced more robustly in cells infected with the mutant viruses. Altogether, these results demonstrate that critical determinants within the nsP1/nsP2 cleavage domain play an important role in regulating alphavirus induced IFN responses.
AAV-6 mediated efficient transduction of mouse lower airways
Creators: name: Pickles, Raymond J.
name: Zhang, Liqun
name: Wu, Zhijian
name: Samulski, R. Jude
name: Li, Wuping
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: AAV1 and AAV6 are two closely related AAV serotypes. In the present study, we found AAV6 was more efficient in transducing mouse lower airway epithelia in vitro and in vivo than AAV1. To further explore the mechanism of this difference, we found that significantly more AAV1 bound to mouse airway epithelia than AAV6, yet transduction by AAV6 was far superior. Lectin competition assays demonstrated that both AAV1 and AAV6 similarly utilize α-2, 3-, and to a lesser extend α-2, 6- linked sialic acids as the receptors for transduction. Furthermore, the rates of AAV endocytosis could not account for the transduction differences of AAV1 and AAV6. Finally, it was revealed that AAV6 was less susceptible to ubiquitin/proteasome-mediated blocks than AAV1 when transducing mouse airway epithelia. Thus compared with AAV1, AAV6 has a unique ability to escape proteasome-mediated degradation, which is likely responsible for its higher transduction efficiency in mouse airway epithelium.
Comparison of differing cytopathic effects in human airway epithelium of parainfluenza virus 5 (W3A), parainfluenza virus type 3, and respiratory syncytial virus
Creators: name: Zhang, Liqun
name: Collins, Peter L.
name: Pickles, Raymond J.
name: Lamb, Robert A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: Parainfluenza virus 5 (PIV5) infects a wide range of animals including dogs, pigs, cats, and humans; however, its association with disease in humans remains controversial. In contrast to parainfluenza virus 3 (PIV3) or respiratory syncytial virus (RSV), PIV5 is remarkably non-cytopathic in monolayer cultures of immortalized epithelial cells. To compare the cytopathology produced by these viruses in a relevant human tissue, we infected an in vitro model of human ciliated airway epithelium and measured outcomes of cytopathology. PIV5, PIV3 and, RSV all infected ciliated cells, and PIV5 and PIV3 infection was dependent on sialic acid residues. Only PIV5-infected cells formed syncytia. PIV5 infection resulted in a more rapid loss of infected cells by shedding of infected cells into the lumen. These studies revealed striking differences in cytopathology of PIV5 versus PIV3 or RSV and indicate the extent of cytopathology determined in cell-lines does not predict events in differentiated airway cells.
Foxp3 inhibits HDAC1 activity to modulate gene expression in human T cells
Creators: name: Gao, Jianmei
name: Holmes, Derek
name: Su, Lishan
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: We have previously reported that HIV-1 preferentially infects Foxp3+ Treg cells in vitro and in vivo, and Foxp3 enhances the HIV-1 LTR expression through epigenetic mechanisms in T cells. We report here that histone deacetylase inhibitor (HDACi) failed to further enhance HIV gene expression in FoxP3+ T cells. We discovered that Foxp3 inhibited cellular HDAC activity in T cells, and mutations in the forkhead domain that ablate Foxp3 function also abolished its ability to inhibit HDAC. When co-expressed, Foxp3 specifically inhibited the deacetylase activity of HDAC1. We further showed that Foxp3 was associated with HDAC1, and mutations in the forkhead domain that ablate Foxp3 function in Treg cells also inhibited Foxp3 association with and inhibition of HDAC1. Finally, Foxp3 failed to enhance HIV-1 gene expression in human T cells expressing HDAC1-specific shRNA. We conclude that Foxp3 modulates gene expression in human T cells at least partly by inhibiting HDAC1 activity.
title: Writing the Good War
Creators: name: Nunziante, Laura Elisa
Year: 2022

description: Diese Arbeit untersucht ausgewählte Reportagen der US-Kriegsberichterstatterinnen Janet Flanner und Lee Miller im Zweiten Weltkrieg anhand feministischer literarischer Diskurse und der Metapherntheorie von Lakoff und Johnson (2003). Flanner und Miller arbeiteten mit Sprachbilder und Metaphern, die sie in ihrer vorherigen Laufbahn entwickelt hatten: Miller schöpfte aus dem Fashion-Ressort der Vogue, für die sie unter anderem über die Befreiung von Dachau berichtete (1945); Flanner etablierte im New Yorker eine High-Class-Gossip-Kolumne und schrieb in diesem Stil unter anderem ein Profil über Hitler (1936). Zeitgenössische Diskurse über binäre Geschlechtermodelle geben weiter Aufschluss darüber, inwieweit die Autorinnen diese übernahmen. Mit Ernest Hemingways How We Came To Paris (1944) wird eine von patriarchaler Schrift (Cixous:1975) geprägte Reportage zum Vergleich herangezogen. Daran wird deutlich, dass Flanner und Miller innerhalb der weiblichen Reportage einen subversiven Beitrag zur US-Kriegsberichterstattung im Zweiten Weltkrieg beisteuerten, der diversere Rollenzuschreibungen zuließ.
description: This thesis examines WWII-dispatches from US-war correspondents Janet Flanner and Lee Miller in respect of feminist and literary discourses as well as the theory of metaphor (Lakoff and Johnson: 2003). Flanner and Miller worked with vivid imagery and metaphors they had developed in their former careers: Miller within the fashion department of Vogue for which she covered the liberation of Dachau (1945); Flanner with a High-Class-Gossip column in the New Yorker, which literary style she used in her profile about Hitler (1936). A comparison with contemporary discourses about binary systems regarding gender and bodies explain further how Flanner and Miller positioned themselves in opposition to them. Ernest Hemingways dispatch How We Came To Paris (1944) is drawn upon for a further comparative analysis regarding male war coverage to show the subversive contribution Janet Flanner and Lee Miller made to discourses about gender and body within the war coverage of WWII.
Topological incommensurate magnetization plateaus in quasi-periodic quantum spin chains
Creators: name: Hu, Hai-Ping
name: Cheng, Chen
name: Luo, Hong-Gang
name: Chen, Shu
Publisher: name: arXiv
Year: 2014
Subjects: subject: Strongly Correlated Electrons (cond-mat.str-el)
subject: FOS: Physical sciences

description: Uncovering topologically nontrivial states in nature is an intriguing and important issue in recent years. While most studies are based on the topological band insulators, the topological state in strongly correlated low-dimensional systems has not been extensively explored due to the failure of direct explanation from the topological band insulator theory on such systems and the origin of the topological property is unclear. Here we report the theoretical discovery of strongly correlated topological states in quasi-periodic Heisenberg spin chain systems corresponding to a series of incommensurate magnetization plateaus under the presence of the magnetic field, which are uniquely determined by the quasi-periodic structure of exchange couplings. The topological features of plateau states are demonstrated by the existence of non-trivial spin-flip edge excitations, which can be well characterized by nonzero topological invariants defined in a two-dimensional parameter space. Furthermore, we demonstrate that the topological invariant of the plateau state can be read out from a generalized Streda formula and the spin-flip excitation spectrum exhibits a similar structure of the Hofstadter's butterfly spectrum for the two-dimensional quantum Hall system on a lattice.
description: 6 pages, 6 figures
Identification of interaction domains within the UL37 tegument protein of herpes simplex virus type 1
Creators: name: Bucks, Michelle A.
name: O'Regan, Kevin J.
name: Murphy, Michael A.
name: Courtney, Richard J.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: Herpes simplex virus type 1 (HSV-1) UL37 is a 1,123 amino acid tegument protein that self-associates and binds to the tegument protein UL36 (VP1/2). Studies were undertaken to identify regions of UL37 involved in these protein-protein interactions. Coimmunoprecipitation assays showed that residues within the carboxy-terminal half of UL37, amino acids 568–1123, are important for interaction with UL36. Coimmunoprecipitation assays also revealed that amino acids 1–300 and 568–1123 of UL37 are capable of self-association. UL37 appears to self-associate only under conditions when UL36 is not present or is present in low amounts, suggesting UL36 and UL37 may compete for binding. Transfection-infection experiments were performed to identify domains of UL37 that complement the UL37 deletion virus, KΔUL37. The carboxy terminal region of UL37 (residues 568–1123) partially rescues the KΔUL37 infection. These results suggest the C-terminus of UL37 may contribute to its essential functional role within the virus-infected cell.
$δ-δ^\prime$ generalized Robin boundary conditions and quantum vacuum fluctuations
Creators: name: Munoz-Castaneda, J. M.
name: Guilarte, J. Mateos
Publisher: name: arXiv
Year: 2014
Subjects: subject: High Energy Physics - Theory (hep-th)
subject: Mathematical Physics (math-ph)
subject: Quantum Physics (quant-ph)
subject: FOS: Physical sciences

description: The effects induced by the quantum vacuum fluctuations of one massless real scalar field on a configuration of two partially transparent plates are investigated. The physical properties of the infinitely thin plates are simulated by means of Dirac-$δ-δ^\prime$ point interactions. It is shown that the distortion caused on the fluctuations by this external background gives rise to a generalization of Robin boundary conditions. The $T$-operator for potentials concentrated on points with non defined parity is computed with total generality. The quantum vacuum interaction energy between the two plates is computed using the $TGTG$ formula to find positive, negative, and zero Casimir energies. The parity properties of the $δ-δ^\prime$ potential allow repulsive quantum vacuum force between identical plates.
description: 21 pages and 11 figures. PhysRevD
Induction and function of virus-specific CD4+ T cell responses
Creators: name: Whitmire, Jason K.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: CD4+ T cells -- often referred to as T-helper cells -- play a central role in immune defense and pathogenesis. Virus infections and vaccines stimulate and expand populations of antigen-specific CD4+ T cells in mice and in man. These virus-specific CD4+ T cells are extremely important in antiviral protection: deficiencies in CD4+ T cells are associated with virus reactivation, generalized susceptibility to opportunistic infections, and poor vaccine efficacy. As described below, CD4+ T cells influence effector and memory CD8+ T cell responses, humoral immunity, and the antimicrobial activity of macrophages and are involved in recruiting cells to sites of infection. This review summarizes a few key points about the dynamics of the CD4+ T cell response to virus infection, the positive role of pro-inflammatory cytokines in the differentiation of virus-specific CD4+ T cells, and new areas of investigation to improve vaccines against virus infection.
Four-point amplitudes in $\mathcal{N}=2$ SCQCD
Creators: name: Leoni, Marta
name: Mauri, Andrea
name: Santambrogio, Alberto
Publisher: name: arXiv
Year: 2014
Subjects: subject: High Energy Physics - Theory (hep-th)
subject: FOS: Physical sciences

description: We compute four-point scattering amplitudes in $\mathcal{N}=2$ SCQCD with general external matter configurations using $\mathcal{N}=1$ superspace Feynman diagrams, at one loop in the general case and up to two loops in the fundamental sector. In the pure adjoint sector at one loop we confirm exact agreement with the corresponding amplitudes in $\mathcal{N}=4$ SYM theory, supporting the idea that a closed subsector of the SCQCD might be exactly integrable. External matter in the fundamental representation breaks dual conformal invariance already at one loop and also the maximum transcendentality principle at two loops.
description: 38 pages, jheppub; v2: minor corrections, JHEP published version; v3: two-loop chiral propagator corrected, maximal transcendentality at two loops not respected anymore, JHEP Erratum
Webinars sobre Buenas Prácticas en los Procesos Editoriales de Revistas Científicas para LILACS (2020) - Sesión III - Tema: Tema: "Flujo Editorial"
Creators: name: BIREME/OPS/OMS
name: Tarragó, Dra. Nancy Sánchez
name: Suga, Sueli Mitiko Yano
Publisher: name: Zenodo
Year: 2023
Subjects: Buenas prácticas
Ciencia aberta
Lilacs
Editores Lilacs
Revista Lilacs
Buenas Prácticas Editoriales

description: (03|09) 𝗙𝗟𝗨𝗝𝗢 𝗘𝗗𝗜𝗧𝗢𝗥𝗜𝗔𝗟 - Buenas Prácticas Procesos Editoriales LILACS 2020 Sesión 03 | 20 de mayo de 2020 • Tema: "Flujo Editorial" • Ponente: Profª Drª Nancy Sánchez Tarragó 00:00 - Presentación (Sueli Suga) 04:25 - Introducción 07:25 - Motivaciones y expectativas de los actores de la Comunicación Científica 14:51- ¿Qué es el proceso o flujo editorial? 20:57 - Etapas generales 22:00 - Participantes en el proceso editorial 24:09 - Funciones del Editor 27:27 - Evaluadores 28:15 - Corrector de Estilo 29:25 - Diseñador / diagramador / maquetador 30:30 - Bibliotecário 33:18 - Etapas del proceso editorial 34:10 - Sistemas de gestión electrónica de manuscritos 35:58 - Envío y evaluación del manuscrito 43:03 - Corrección y diagramación 47:26 - Publicación 48:23 - Modelo de producción editorial tradicional: número completo 49:39 - Publicación antecipada 51:00 - Publicación contínua 54:30 - Divulgación 56:06 - Indexación 56:45 - LILACS-Express 59:50 - Medición de impacto y relevancia del contenido 01:01:40 - Revistas y repositorios de preprints 01:04:04 - Conclusión 01:05:22 - Preguntas de los participantes 01:36:50 - Cierre de la sesión Más informaciones → https://lilacs.bvsalud.org/es/buenas-practicas-en-los-procesos-editoriales-de-revistas-cientificas-para-lilacs-2020/
The Epstein–Barr Virus BART microRNAs target the pro-apoptotic protein Bim
Creators: name: Mathur, Anuja
name: Marquitz, Aron R.
name: Nam, Cyd Stacy
name: Raab-Traub, Nancy
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: In Epstein-Barr virus infected epithelial cancers, the alternatively spliced BamHI A rightward transcripts (BARTs) are abundantly expressed and are the template for two large clusters of miRNAs. This study indicates that both of these clusters independently can inhibit apoptosis in response to etoposide in an epithelial cell line. The Bcl-2 interacting mediator of cell death (Bim) was identified using gene expression microarrays and bioinformatic analysis indicated multiple potential binding sites for several BART miRNAs in the Bim 3’UTR Bim protein was reduced by Cluster I and the individual expression of several miRNAs, while mRNA levels were unaffected. In reporter assays, the Bim 3' untranslated region (UTR) was inhibited by both clusters but not by any individual miRNAs. These results are consistent with the BART miRNAs downregulating Bim post-transcriptionally in part through the 3’UTR and suggest that there are miRNA recognition sites within other areas of the Bim mRNA.
Antibodies targeting dengue virus envelope domain III are not required for serotype-specific protection or prevention of enhancement in vivo
Creators: name: Orozco, Susana
name: Harris, Eva
name: Wahala, Wahala M.P.B.
name: de Silva, Aravinda M.
name: Williams, Katherine L.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2012

description: The envelope (E) protein of dengue virus (DENV) is composed of three domains (EDI, EDII, EDIII) and is the main target of neutralizing antibodies. Many monoclonal antibodies that bind EDIII strongly neutralize DENV. However in vitro studies indicate that anti-EDIII antibodies contribute little to the neutralizing potency of human DENV-immune serum. In this study, we assess the role of anti-EDIII antibodies in mouse and human DENV-immune serum in neutralizing or enhancing DENV infection in mice. We demonstrate that EDIII-depleted human DENV-immune serum was protective against homologous DENV infection in vivo. Although EDIII-depleted DENV-immune mouse serum demonstrated decreased neutralization potency in vitro, reduced protection in some organs, and enhanced disease in vivo, administration of increased volumes of EDIII-depleted serum abrogated these effects. These data indicate that anti-EDIII antibodies contribute to protection and minimize enhancement when present, but can be replaced by neutralizing antibodies targeting other epitopes on the dengue virion.
Human parainfluenza virus serotypes differ in their kinetics of replication and cytokine secretion in human tracheobronchial airway epithelium
Creators: name: Schaap-Nutt, Anne
name: Liesman, Rachael
name: Collins, Peter L.
name: Scull, Margaret A.
name: Bartlett, Emmalene J.
name: Schmidt, Alexander C.
name: Pickles, Raymond J.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2012

description: Human parainfluenza viruses (PIVs) cause acute respiratory illness in children, the elderly, and immunocompromised patients. PIV3 is a common cause of bronchiolitis and pneumonia, whereas PIV1 and 2 are frequent causes of upper respiratory tract illness and croup. To assess how PIV1, 2, and 3 differ with regard to replication and induction of type I interferons, interleukin-6, and relevant chemokines, we infected primary human airway epithelium (HAE) cultures from the same tissue donors and examined replication kinetics and cytokine secretion. PIV1 replicated to high titer yet did not induce cytokine secretion until late in infection, while PIV2 replicated less efficiently but induced an early cytokine peak. PIV3 replicated to high titer but induced a slower rise in cytokine secretion. The T cell chemoattractants CXCL10 and CXCL11 were the most abundant chemokines induced. Differences in replication and cytokine secretion might explain some of the differences in PIV serotype-specific pathogenesis and epidemiology.
Analog geometry in an expanding fluid from AdS/CFT perspective
Creators: name: Bilić, Neven
name: Domazet, Silvije
name: Tolić, Dijana
Publisher: name: arXiv
Year: 2014
Subjects: subject: High Energy Physics - Theory (hep-th)
subject: General Relativity and Quantum Cosmology (gr-qc)
subject: FOS: Physical sciences

description: The dynamics of an expanding hadron fluid at temperatures below the chiral transition is studied in the framework of AdS/CFT correspondence. We establish a correspondence between the asymptotic AdS geometry in the 4+1-dimensional bulk with the analog spacetime geometry on its 3+1 dimensional boundary with the background fluid undergoing a spherical Bjorken type expansion. The analog metric tensor on the boundary depends locally on the soft pion dispersion relation and the four-velocity of the fluid. The AdS/CFT correspondence provides a relation between the pion velocity and the critical temperature of the chiral phase transition.
description: 16 pages, 2 figures, revised to match the published version, relation to static solutions clarified, the section on de Sitter boundary removed, results unchanged
Vironome of Kaposi sarcoma associated herpesvirus-inflammatory cytokine syndrome in an AIDS patient reveals co-infection of human herpesvirus 8 and human herpesvirus 6A
Creators: name: van der Horst, Charles
name: Dittmer, Dirk P.
name: Moll, Stephan
name: Damania, Blossom
name: Lucas, Amy
name: Yang, Dongmei
name: Malouf, Nadia
name: Moylan, Vincent
name: Sin, Sang-Hoon
name: Tamburro, Kristen M.
name: Poisson, Jessica
name: Fedoriw, Yuri
name: Roy, Debasmita
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2012

description: KSHV inflammatory cytokine syndrome (KICS) is a newly described condition characterized by systemic illness as a result of systemic, lytic KSHV infection. We used Illumina sequencing to establish the DNA vironome of blood from such a patient. It identified concurrent high-level viremia of human herpesvirus (HHV) 8 and 6a. The HHV8 plasma viral load was 5,300,000 copies/ml, which is the highest reported to date; this despite <5 skin lesions and no HHV8 associated lymphoma. This is the first report of systemic HHV6a/KSHV co-infection in a patient. It is the first whole genome KSHV sequence to be determined directly from patient plasma rather than cultured or biopsied tumor material. This case supports KICS as a new clinical entity associated with KSHV.
An attenuating mutation in a neurovirulent Sindbis virus strain interacts with the IPS-1 signaling pathway in vivo
Creators: name: Wollish, Amy C.
name: Heise, Mark T.
name: Ferris, Martin T.
name: Loo, Yueh-Ming
name: Gale, Michael
name: Blevins, Lance K.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2013

description: The AR86 strain of Sindbis virus causes lethal neurologic disease in adult mice. Previous studies have identified a virulence determinant at nonstructural protein (nsP) 1 position 538 that regulates neurovirulence, modulates clearance from the CNS, and interferes with the type I interferon pathway. The studies herein demonstrate that in the absence of type I interferon signaling, the attenuated mutant exhibited equivalent virulence to S300 virus. Furthermore, both S300 and nsP1 T538I viruses displayed similar neurovirulence and replication kinetics in IPS-1-/- mice. TRIF dependent signaling played a modest role in protecting against disease by both S300 and nsP1 T538I, but did not contribute to control of nsP1 T538I replication within the CNS, while MyD88 played no role in the disease process. These results indicate that the control of the nsP1 T538I mutant virus is largely mediated by IPS-1-dependent RLR signaling, with TRIF-dependent TLR signaling also contributing to protection from virus-induced neurologic disease.
A New Observable for Identifying Dijet Resonances
Creators: name: Izaguirre, Eder
name: Shuve, Brian
name: Yavin, Itay
Publisher: name: arXiv
Year: 2014
Subjects: subject: High Energy Physics - Phenomenology (hep-ph)
subject: High Energy Physics - Experiment (hep-ex)
subject: FOS: Physical sciences

description: The development of techniques for identifying hadronic signals from the overwhelming multi-jet backgrounds is an important part of the Large Hadron Collider (LHC) program. Of prime importance are resonances decaying into a pair of partons, such as the Higgs and $\rm W$/$\rm Z$ bosons, as well as hypothetical new particles. We present a simple observable to help discriminate a dijet resonance from background that is effective even when the decaying resonance is not strongly boosted. We find consistent performance of the observable over a variety of processes and degree of boosts, and show that it leads to a reduction of the background by a factor of $3-5$ relative to signal at the price of $10-20\%$ signal efficiency. This approach represents a significant increase in sensitivity for Standard Model (SM) measurements and searches for new physics that are dominated by systematic uncertainties, which is true of many analyses involving jets - particularly in the high-luminosity running of the LHC.
description: 6 pages, 6 figures. Version to appear in PRL
数据过程.zip
Creators: name: 黄, 志龙
Publisher: name: figshare
Year: 2023
Subjects: classificationCode: 321104
subject: Cancer therapy (excl. chemotherapy and radiation therapy)

description: We aim to conduct a critical review of only the currently available evidence from randomised controlled studies comparing the effectiveness, safety and clinical outcomes (O)of robotic surgery (I) versus traditional laparoscopic surgery (C) for the excision of colorectal cancer in adult patients (P)
Emergence potential of sylvatic dengue virus type 4 in the urban transmission cycle is restrained by vaccination and homotypic immunity
Creators: name: Munoz-Jordan, Jorge L.
name: Jane Cardosa, M.
name: Tesh, Robert B.
name: Rossi, Shannan L.
name: Ramos-Castaneda, Jose
name: Durbin, Anna P.
name: Eong Ooi, Eng
name: Messer, William B.
name: Weaver, Scott C.
name: Vasilakis, Nikos
name: Mayer, Sandra V.
name: Amaya-Larios, Irma Y.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2013

description: Sylvatic dengue viruses (DENV) are both evolutionarily and ecologically distinct from human DENV and are maintained in an enzootic transmission cycle. Evidence of sylvatic human infections from West Africa and Southeast Asia suggests that sylvatic DENV come into regular contact with humans. Thus, this potential of emergence into the human transmission cycle could limit the potential for eradicating this cycle with vaccines currently in late stages of development. We assessed the likelihood of sylvatic DENV-4 emergence in the face of natural immunity to current human strains and vaccination with two DENV-4 vaccine candidates. Our data indicate homotypic neutralization of sylvatic and human DENV-4 strains by human primary convalescent and vaccinee sera but limited heterotypic immunity. These results suggest that emergence of sylvatic strains into the human cycle would be limited by homotypic immunity mediated by virus neutralizing antibodies produced by natural infection or vaccination.
Snapshots: Chromatin control of viral infection
Creators: name: Margolis, David
name: McBride, Alison A.
name: Jung, Jae U.
name: Nieto, Amelia
name: Knipe, David M.
name: Morris, Kevin V.
name: Nevels, Michael
name: Ott, Melanie
name: Kristie, Thomas M.
name: Lieberman, Paul M.
name: Parks, Robin J.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2013

description: Like their cellular host counterparts, many invading viral pathogens must contend with, modulate, and utilize the host cell’s chromatin machinery to promote efficient lytic infection or control persistent-latent states. While not intended to be comprehensive, this review represents a compilation of conceptual snapshots of the dynamic interplay of viruses with the chromatin environment. Contributions focus on chromatin dynamics during infection, viral circumvention of cellular chromatin repression, chromatin organization of large DNA viruses, tethering and persistence, viral interactions with cellular chromatin modulation machinery, and control of viral latency-reactivation cycles.
The mechanism of differential neutralization of dengue serotype 3 strains by monoclonal antibody 8A1
Creators: name: Messer, William B.
name: de Silva, Aravinda M.
name: Huynh, Jeremy P.
name: Zhou, Yang
name: Yount, Boyd L.
name: Baric, Ralph S.
name: Fremont, Daved H.
name: Austin, S.Kyle
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2013

description: While previous studies have demonstrated that envelope (E) glycoprotein variation between dengue viruses (DENV) genotypes can influence antibody neutralization potency, the mechanisms of variable neutralization remain incompletely understood. Here we characterize epitope antibody interactions of a DENV-3 EDIII binding mouse mAb 8A1 which displays highly variable neutralizing activity against DENV-3 genotypes. Using a DENV-3 reverse genetics platform, we characterize ability of 8A1 to bind and neutralize naturally occurring DENV-3 E genotypic variant viruses. Introduction of single and multiple amino acid mutations into the parental clone background demonstrates that mutations at positions 301 and 383 on EDIII are responsible for 8A1 differential neutralization phenotypes. ELISA and surface plasmon resonance (SPR) studies indicate differences in binding are responsible for the variable neutralization. Variability at position 301 primarily determined binding difference through influencing antibody-EDIII dissociation rate. Our findings are relevant to the many groups focusing on DENV EDIII as a vaccine target.
Entropic one-class classifiers
Creators: name: Livi, Lorenzo
name: Sadeghian, Alireza
name: Pedrycz, Witold
Publisher: name: arXiv
Year: 2014
Subjects: subject: Computer Vision and Pattern Recognition (cs.CV)
subject: Machine Learning (cs.LG)
subject: Machine Learning (stat.ML)
subject: FOS: Computer and information sciences
subject: I.2.6; K.2.3

description: The one-class classification problem is a well-known research endeavor in pattern recognition. The problem is also known under different names, such as outlier and novelty/anomaly detection. The core of the problem consists in modeling and recognizing patterns belonging only to a so-called target class. All other patterns are termed non-target, and therefore they should be recognized as such. In this paper, we propose a novel one-class classification system that is based on an interplay of different techniques. Primarily, we follow a dissimilarity representation based approach; we embed the input data into the dissimilarity space by means of an appropriate parametric dissimilarity measure. This step allows us to process virtually any type of data. The dissimilarity vectors are then represented through a weighted Euclidean graphs, which we use to (i) determine the entropy of the data distribution in the dissimilarity space, and at the same time (ii) derive effective decision regions that are modeled as clusters of vertices. Since the dissimilarity measure for the input data is parametric, we optimize its parameters by means of a global optimization scheme, which considers both mesoscopic and structural characteristics of the data represented through the graphs. The proposed one-class classifier is designed to provide both hard (Boolean) and soft decisions about the recognition of test patterns, allowing an accurate description of the classification process. We evaluate the performance of the system on different benchmarking datasets, containing either feature-based or structured patterns. Experimental results demonstrate the effectiveness of the proposed technique.
description: To appear in IEEE-TNNLS
Partial facial reduction: simplified, equivalent SDPs via approximations of the PSD cone
Creators: name: Permenter, Frank
name: Parrilo, Pablo
Publisher: name: arXiv
Year: 2014
Subjects: subject: Optimization and Control (math.OC)
subject: FOS: Mathematics

description: We develop a practical semidefinite programming (SDP) facial reduction procedure that utilizes computationally efficient approximations of the positive semidefinite cone. The proposed method simplifies SDPs with no strictly feasible solution (a frequent output of parsers) by solving a sequence of easier optimization problems and could be a useful pre-processing technique for SDP solvers. We demonstrate effectiveness of the method on SDPs arising in practice, and describe our publicly-available software implementation. We also show how to find maximum rank matrices in our PSD cone approximations (which helps us find maximal simplifications), and we give a post-processing procedure for dual solution recovery that generally applies to facial-reduction-based pre-processing techniques. Finally, we show how approximations can be chosen to preserve problem sparsity.
description: Expanded computational results, added results on sparsity, and revised dual solution recovery section
Each of the eight simian hemorrhagic fever virus minor structural proteins is functionally important
Creators: name: Donaldson, Eric F.
name: Vatter, Heather A.
name: Di, Han
name: Brinton, Margo A.
name: Baric, Ralph S.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2014

description: The simian hemorrhagic fever virus (SHFV) genome differs from those of other members of the family Arterivirus in encoding two adjacent sets of four minor structural protein open reading frames (ORFs). A stable, full-length, infectious SHFV-LVR cDNA clone was constructed. Virus produced from this clone had replication characteristics similar to those of the parental virus. A subgenomic mRNA was identified for the SHFV ORF previously identified as 2b. As an initial means of analyzing the functional relevance of each of the SHFV minor structural proteins, a set of mutant infectious clones was generated, each with the start codon of one minor structural protein ORF mutated. Different phenotypes were observed for each ortholog of the pairs of minor glycoproteins and all of the eight minor structural proteins were required for the production of infectious extracellular virus indicating that the duplicated sets of SHFV minor structural proteins are not functionally redundant.
Calculation of oscillation probabilities of atmospheric neutrinos using nuCraft
Creators: name: Wallraff, Marius
name: Wiebusch, Christopher
Publisher: name: arXiv
Year: 2014
Subjects: subject: Instrumentation and Methods for Astrophysics (astro-ph.IM)
subject: High Energy Physics - Phenomenology (hep-ph)
subject: Computational Physics (physics.comp-ph)
subject: FOS: Physical sciences

description: NuCraft (nucraft.hepforge.org) is an open-source Python project that calculates neutrino oscillation probabilities for neutrinos from cosmic-ray interactions in the atmosphere for their propagation through Earth. The solution is obtained by numerically solving the Schrödinger equation. The code supports arbitrary numbers of neutrino flavors including additional sterile neutrinos, CP violation, arbitrary mass hierarchies, matter effects with a configurable Earth model, and takes into account the production height distribution of neutrinos in the Earth's atmosphere.
description: submitted to Computer Physics Communications
ATTITUDES ON POPULISM: A COMPARATIVE ENQUIRY ACROSS COUNTRIES AND PARTIES
Creators: name: ESTEBAN PEREZ, IRENE
Publisher: name: Universit� degli Studi di Milano
Year: 2019

description: This thesis addresses populism from the demand side. The dissertation focuses on populist attitudes, their different dimensions, interactions with other political attitudes and the relationship between different types of populism and different populist attitudes. In sum, this work aims to fill a gap in the literature with the exploration and expansion of populist attitudes in different European contexts.
Deep Learning for Reliable Soft Sensor Development
Creators: name: DING, ZE YANG
Publisher: name: Monash University
Year: 2023
Subjects: Deep learning
Neural networks

description: Soft sensors are widely used in industrial systems to monitor key variables that are difficult to measure, using measurements from other available physical sensors. This thesis aims to develop efficient modeling strategies using deep learning methods to construct data-driven soft sensors that are both accurate and reliable. The outcomes of this research have significant impacts towards advancing the state-of-the-art of deep learning-based soft sensors by enhancing their practicality in modern industries.