Additional file 1 of A mitochondria-targeted molecular phototheranostic platform for NIR-II imaging-guided synergistic photothermal/photodynamic/immune therapy
Creators: name: Yang, Sha
name: Wu, Gui-long
name: Li, Na
name: Wang, Minghui
name: Wu, Peixian
name: He, Yuxuan
name: Zhou, Wei
name: Xiao, Hao
name: Tan, Xiaofeng
name: Tang, Li
name: Yang, Qinglai
Publisher: name: figshare
Year: 2022
Subjects: Space Science
Medicine
Cell Biology
Chemical Sciences not elsewhere classified
Science Policy
Information Systems not elsewhere classified
Cancer
Computational Biology

description: Additional file 1: Fig. S1. Synthetic routes of FEPT and FEP. Fig. S2. 1H NMR of compound 2. Fig. S3. 13C NMR of compound 2. Fig. S4. 1H NMR of compound 5. Fig. S5. 13C NMR of compound 5. Fig. S6. 1H NMR of compound IR-FE. Fig. S7. 13C NMR of compound IR-FE. Fig. S8. 1H NMR of compound 8. Fig. S9. 13C NMR of compound 8. Fig. S10. 1H NMR of compound FEPT. Fig. S11. 13C NMR of compound FEPT. Fig. S12. 13P NMR of compound FEPT. Fig. S13. HRMS of compound FEPT. Fig. S14. 1H NMR of compound FEP. Fig. S15. 13C NMR of compound FEP. Fig. S16. HRMS of compound FEP. Fig. S17. A UV–Vis absorbance spectra of FEP and FEPT. B UV–Vis absorbance of FEP (black line) and NIR-II fluorescence emission of FEP (red line) with a peak at 1085 nm under 808 nm laser excitation (solvents: water). C The fluorescence emission of FEP (red line) and FEPT (black line) under 405 nm laser excitation (solvents: water). D Photothermal conversion performance curves of FEP. Fig. S18. A UV–Vis absorbance stability of FEPT in different PH resolution. B NIR-II fluorescence stability of FEPT with a peak at 1085 nm under 808 nm laser excitation (solvents: different PH solutions). C The size of FEPT measured by dynamic light scattering (solvents: water). D The TEM of FEPT in water. Scale bar:100 nm. Fig. S19. Cellular uptake images of FEPT in 4T1 cells captured by LSM980. Scale bar: 20 μm. Fig. S20. A Mean fluorescence intensity of ROS in 4T1 cells detected with DCFH-DA probe after different treatments. B Changes in mitochondrial membrane potential (ΔΨm) of the relative fluorescent intensity of JC-1 aggregates and monomer in 4T1 cells with different treatments. C Quantitative fluorescence intensity of cleaved caspase-3 in 4T1 cells with different treatments analyzed by CLSM. D Cleaved caspase-3 detection of 4T1 cells after different treatments by ELISA. Error bars: mean ± SD (n = 3). *P &lt; 0.05; **P &lt; 0.01; ***P &lt; 0.001. Fig. S21. H&amp;E staining images of five major organs (heart, liver, spleen, lung and kidney) in the healthy Balb/c mice after intravenous injection of PBS, FEP and FEPT without 808 nm laser irradiation at 15 d  post-injection. Scale bar: 50 μm. Fig. S22. A Blood routine examinations in the PBS, FEP and FEPT groups. B Chemistry indexes of the liver and renal function tests in the PBS, FEP and FEPT groups. All the tests are detected in the healthy Balb/c mice without NIR irradiation at 15 d post-injection. Fig. S23. H&amp;E staining images of five major organs (heart, liver, spleen, lung, and kidneys) in 4T1 tumor-bearing mice with different treatments at 15 d post-injection. Scale bar: 50 μm. Fig. S24. Quantitative results of CD3+CD4+T cells, CD3+CD8+T cells, and Tregs in CD4+T cells in splenic lymphocytes isolated from different-treated mice. Mean ± SD (n = 3). *P &lt; 0.05; **P &lt; 0.01; ***P &lt; 0.001. Fig. S25. The IHC analysis of Tregs and the IF analysis of infiltrating CD4+T (green) and CD8+T (red) cells in the spleen and tumor tissues. The blue signals in IHC and IF staining indicate cellular nuclei that stained with hematoxylin and Hoechst 33258, respectively. The positive signal of Tregs in IHC staining is brown. Green and red fluorescence signal indicates CD4+T, CD8+T cells, respectively. Scale bar: 50 μm.