cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
BRCA1 and FancJ cooperatively promote interstrand crosslinker induced centrosome amplification through the activation of polo-like kinase 1
Creators: name: Zou, Jianqiu
name: Zhang, Deli
name: Qin, Guang
name: Chen, Xiangming
name: Wang, Hongmin
name: Zhang, Dong
Publisher: name: Taylor & Francis
Year: 2014
Subjects: subject: Cancer
subject: Biological Sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: Molecular Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Cell Biology
subject: Biochemistry

description: DNA damage response (DDR) and the centrosome cycle are 2 of the most critical cellular processes affecting the genome stability in animal cells. Yet the cross-talks between DDR and the centrosome are poorly understood. Here we showed that deficiency of the breast cancer 1, early onset gene (BRCA1) induces centrosome amplification in non-stressed cells as previously reported while attenuating DNA damage-induced centrosome amplification (DDICA) in cells experiencing prolonged genotoxic stress. Mechanistically, the function of BRCA1 in promoting DDICA is through binding and recruiting polo-like kinase 1 (PLK1) to the centrosome. In a recent study, we showed that FancJ also suppresses centrosome amplification in non-stressed cells while promoting DDICA in both hydroxyurea and mitomycin C treated cells. FancJ is a key component of the BRCA1 B-complex. Here, we further demonstrated that, in coordination with BRCA1, FancJ promotes DDICA by recruiting both BRCA1 and PLK1 to the centrosome in the DNA damaged cells. Thus, we have uncovered a novel role of BRCA1 and FancJ in the regulation of DDICA. Dysregulation of DDR or centrosome cycle leads to aneuploidy, which is frequently seen in both solid and hematological cancers. BRCA1 and FancJ are known tumor suppressors and have well-recognized functions in DNA damage checkpoint and DNA repair. Together with our recent findings, we demonstrated here that BRCA1 and FancJ also play an important role in centrosome cycle especially in DDICA. DDICA is thought to be an alternative fail-safe mechanism to prevent cells experiencing severe DNA damage from becoming carcinogenic. Therefore, BRCA1 and FancJ are potential liaisons linking early DDR with the DDICA. We propose that together with their functions in DDR, the role of BRCA1 and FancJ in the activation of DDICA is also crucial for their tumor suppression functions in vivo.
A New Efficient Synthesis of 4-Alkoxy-1,6-diaryl-1H-pyrazolo[3,4-d]pyrimidine Derivatives
Creators: name: Liu, Ju
name: Zhang, Xin-wei
name: Wang, Yang
name: Chen, Ye
name: Zhang, Mei-rong
name: Cai, Zhi-qiang
name: Zhou, Yun-peng
name: Xu, Li-feng
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Ecology
subject: FOS: Biological sciences
subject: Biotechnology
subject: Physics
subject: Biochemistry
subject: Biological Sciences
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Cell Biology

description: A new synthesis of 4-methoxy (or ethoxy)-1,6-diaryl-1H-pyrazolo[3,4-d]pyrimidine derivatives is described. An efficient one-step methodology for the title compounds was achieved by using 5-amino-1-aryl-1H-pyrazole-4-carbonitriles with arylaldehydes in CH3OH or C2H5OH and NaOH at 60 °C. The new compounds were obtained in moderate to good yields as stable solids and easily purified by filtration.
Evaluating the quality of radiotherapy treatment plans with uncertainty using data envelopment analysis
Creators: name: Stubington, Emma
Publisher: name: Lancaster University
Year: 2020

description: External beam radiation therapy is a common treatment method for cancer. Radiotherapy is planned with the aim of achieving conflicting goals: while a sufficiently high dose of radiation is necessary for tumour control, a low dose of radiation is desirable to avoid complications in normal, healthy, tissue. This thesis aims to support the radiotherapy treatment planning process for prostate cancer by evaluating the quality of proposed treatment plans relative to previous plans. We develop a variable selection technique, autoPCA, to select the most relevant variables for use in our Data Envelopment Analysis (DEA) models. This allows us to evaluate how well plans perform in terms of achieving the conflicting goals of radiotherapy. We develop the uncertain DEA problem (uDEA) for the case of box uncertainty and show that for small problems this can be solved exactly. This study of uncertainty is motivated by the inherently uncertain nature of the treatment process. Robust DEA, uDEA and simulation are applied to prostate cancer treatment plans to investigate this uncertainty. We identify plans that have the potential to be improved, which clinicians then replan for us. Small improvements were seen and we discuss the potential difference this could make to planning cases that are more complex. To aid this, we develop a prototype software, EvaluatePlan, that assesses the efficiency of a plan compared to past treatment plans.
A New Efficient Synthesis of 4- Alkoxy-1,6-diaryl-1H-pyrazolo[3,4-d]pyrimidine Derivatives
Creators: name: Liu, Ju
name: Zhang, Xin-wei
name: Wang, Yang
name: Chen, Ye
name: Zhang, Mei-rong
name: Cai, Zhi-qiang
name: Zhou, Yun-peng
name: Xu, Li-feng
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Ecology
subject: FOS: Biological sciences
subject: Biotechnology
subject: Physics
subject: Biochemistry
subject: Biological Sciences
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Cell Biology

description: GRAPHICAL ABSTRACT
edxia through the Glue interface
Creators: name: Georget, Fabien
name: Wilson, William
name: Scrivener, Karen
Publisher: name: Zenodo
Year: 2020
Subjects: SEM-EDS
image analysis
edxia
Glueviz
Tutorial

description: This documentation explains how to analyse a SEM-EDS hypermap using edxia and Glue.
Elemental mercury oxidation in an electrostatic precipitator enhanced with in situ soft X-ray irradiation
Creators: name: Jing, He
name: Wang, Xiaofei
name: Wang, Wei-Ning
name: Biswas, Pratim
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Physiology
subject: FOS: Biological sciences
subject: Physics
subject: Biophysics
subject: Plant Biology
subject: Ecology
subject: Chemistry
subject: Earth and Environmental Sciences

description: Corona discharge based techniques are promising approaches for oxidizing elemental mercury (Hg0) in flue gas from coal combustion. In this study, in-situ soft X-rays were coupled to a DC (direct current) corona-based electrostatic precipitator (ESP). The soft X-rays significantly enhanced Hg0 oxidation, due to generation of electrons from photoionization of gas molecules and the ESP electrodes. This coupling technique worked better in the positive corona discharge mode because more electrons were in the high energy region near the electrode. Detailed mechanisms of Hg0 oxidation are proposed and discussed based on ozone generation measurements and Hg0 oxidation behavior observations in single gas environments (O2, N2, and CO2). The effect of O2 concentration in flue gas, as well as the effects of particles (SiO2, TiO2, and KI) was also evaluated. In addition, the performance of a soft X-rays coupled ESP in Hg0 oxidations was investigated in a lab-scale coal combustion system. With the ESP voltage at +10 kV, soft X-ray enhancement, and KI addition, mercury oxidation was maximized.Implications: Mercury is a significant-impact atmospheric pollutant due to its toxicity. Coal-fired power plants are the primary emission sources of anthropogenic releases of mercury; hence, mercury emission control from coal-fired power plant is important. This study provides an alternative mercury control technology for coal-fired power plants. The proposed electrostatic precipitator with in situ soft X-rays has high efficiency on elemental mercury conversion. Effects of flue gas conditions (gas compositions, particles, etc.) on performance of this technology were also evaluated, which provided guidance on the application of the technology for coal-fired power plant mercury control.
Enhanced solubility of piperine using hydrophilic carrier-based potent solid dispersion systems
Creators: name: Kathavarayan Thenmozhi
name: Yoo, Young Je
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Biochemistry
subject: Space Science
subject: Medicine
subject: Pharmacology
subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences

description: Context: Piperine alkaloid, an important constituent of black pepper, exhibits numerous therapeutic properties, whereas its usage as a drug is limited due to its poor solubility in aqueous medium, which leads to poor bioavailability. Objective: Herein, a new method has been developed to improve the solubility of this drug based on the development of solid dispersions with improved dissolution rate using hydrophilic carriers such as sorbitol (Sor), polyethylene glycol (PEG) and polyvinyl pyrrolidone K30 (PVP) by solvent method. Physical mixtures of piperine and carriers were also prepared for comparison. Methods: The physicochemical properties of the prepared solid dispersions were examined using SEM, TEM, DSC, XRD and FT-IR. In vitro dissolution profile of the solid dispersions was recorded and compared with that of the pure piperine and physical mixtures. The effect of these carriers on the aqueous solubility of piperine has been investigated. Results: The solid dispersions of piperine with Sor, PEG and PVP exhibited superior performance for the dissolution of piperine with a drug release of 70%, 76% and 89%, respectively after 2 h compared to physical mixtures and pure piperine, which could be due to its transformation from crystalline to amorphous form as well as the attachment of hydrophilic carriers to the surface of poorly water-soluble piperine. Conclusion: Results suggest that the piperine solid dispersions prepared with improved in vitro release exhibit potential advantage in delivering poorly water-soluble piperine as an oral supplement.
DNA binding, cleavage, and cytotoxicity of binuclear phenolate nickel(II) complexes
Creators: name: Li, Dong-Dong
name: Zhao, Xiu-Mei
name: Gu, Na
name: Zhi, Shuang
name: Zun-Wei Tao
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Biophysics
subject: Biochemistry
subject: Cell Biology
subject: Molecular Biology
subject: Pharmacology
subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Cancer

description: Three binuclear phenolate complexes, [Ni2(L1)2(OAc)](BPh4)·DMF (1), [Ni2(L2)2(OAc)](BPh4) (2), and [Ni2(L3)2(OAc)](OH)·3H2O (3), where L1 = 2-{[bis-(2-hydroxy-ethyl)-amino]-methyl}-4-methyl-phenol, L2 = 2-{[bis-(2-hydroxy-ethyl)-amino]-methyl}-4-methoxy-phenol, and L3 = 2-{[bis-(2-hydroxy-ethyl)-amino]-methyl}-4-tert-butyl-phenol), have been synthesized. Single-crystal diffraction reveals that all the metal atoms are in a distorted octahedral geometry. The interactions of the complexes with calf thymus DNA (CT-DNA) have been investigated by UV–vis absorption, fluorescence emission, and circular dichroism spectroscopy and viscosity measurements. Furthermore, DNA cleavage mechanism shows that the complexes may be capable to promote DNA cleavage through oxidative DNA damage pathway, which is indicative of the involvement of hydroxyl radical, singlet oxygen, or singlet oxygen-like entity in the cleavage process. Cytotoxicity studies on the Hela and MCF-7 cancer cell lines show that complexes 1–3 exhibit excellent activity toward the tested tumor cell lines with respect to the standard drug carboplatin, revealing that they have the potential to act as effective metal-based anticancer drugs.
Elemental Mercury Oxidation in an Electrostatic Precipitator Enhanced with In Situ Soft X-ray Irradiation
Creators: name: Jing, He
name: Wang, Xiaofei
name: Wang, Wei-Ning
name: Biswas, Pratim
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Physiology
subject: FOS: Biological sciences
subject: Physics
subject: Biophysics
subject: Plant Biology
subject: Ecology
subject: Chemistry
subject: Earth and Environmental Sciences

description: IMPLICATIONSMercury is a significant-impact atmospheric pollutant due to its toxicity. Coal-fired power plants are the primary emission sources of anthropogenic releases of mercury, hence mercury emission control from coal-fired power plant is important. This study provides an alternative mercury control technology for coal-fired power plants. The proposed electrostatic precipitator with in situ soft X-rays has high efficiency on elemental mercury conversion. Effects of flue gas conditions (gas compositions, particles, etc.) on performance of this technology were also evaluated, which provided guidance on the application of the technology for coal-fired power plant mercury control.
Blended learning and Syrian refugees' empowerment through a capability approach lens
Creators: name: Abdelfattah Ahmed Younes, Mona
Publisher: name: Lancaster University
Year: 2020
Subjects: Blended Learning; Refugees; Syrian Refugees; Empowerment; Capability Approach

description: This study analyses the learning experiences of three Syrian refugee youth who enrolled in and completed blended learning (BL) programmes in Jordan, in order to explicate how BL has/or has not empowered those refugees. To achieve this objective, the following research questions framed this study: Does blended learning empower Syrian refugee youth living in Jordan? 1. Is blended learning, in the case of Syrian refugee youth, an empowering capability? 2. Does blended learning help Syrian refugee youth to overcome their restrictive ‘rules of the game’? 3. Does blended learning improve Syrian refugee youth’s resource portfolio? This study followed a case study approach. Three Syrian youth, each of whom attended a different BL programme, were interviewed between December 2018 and March 2019. While all three cases expressed aspirations that are education-bound, this study shows some difference in the aspirations of males and females and of urban refugees versus those residing in the camps. BL has been for all three cases a feasible learning opportunity. Programme providers designed the courses in a manner that accommodated to refugees’ locations, time, and economic status. Social media disseminated information about educational opportunities and possessing smartphones made following up with lectures and assignments possible and easy. The investigated BL programmes proved also to be enjoyable due to their constructive, learner-centred and collaborative approach, and competent facilitators. However, this finding does not indicate that those programmes were “empowering”. Restrictive legislations that constrain refugees’ work, movement, and lives, as well as patriarchal traditions that hampered females’ choices of education, were “rules of the game” that hindered students’ ability to fully benefit from the provided programmes. Despite the development of oral and public speaking, employability, and English language skills and despite the enhancement of self-esteem, confidence, and respect towards others, those programmes did not lead to refugees’ full empowerment.
Requirement for CRIF1 in RNA interference and Dicer-2 stability
Creators: name: Jun Lim, Su
name: Scott, Anthony
name: Xiong, Xiao-Peng
name: Vahidpour, Shabnam
name: Karijolich, John
name: Guo, Dongdong
name: Pei, Shanshan
name: Yu, Yi-Tao
name: Zhou, Rui
name: X Li, Willis
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Virology
subject: Hematology
subject: Mental Health
subject: Developmental Biology
subject: Biological Sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: Earth and Environmental Sciences
subject: Molecular Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Cell Biology
subject: Microbiology
subject: Biochemistry
subject: Biophysics

description: RNA interference (RNAi) is a eukaryotic gene-silencing system. Although the biochemistry of RNAi is relatively well defined, how this pathway is regulated remains incompletely understood. To identify genes involved in regulating the RNAi pathway, we screened for genetic mutations in Drosophila that alter the efficiency of RNAi. We identified the Drosophila homolog of the mammalian CR6-interacting factor 1 (CRIF1), also known as growth arrest and DNA-damage-inducible 45-gamma interacting protein (Gadd45GIP1), as a potential new regulator of the RNAi pathway. Loss-of-function mutants of Drosophila CRIF1 (dCRIF) are deficient in RNAi-mediated target gene knock-down, in the biogenesis of small interfering RNA (siRNA) molecules, and in antiviral immunity. Moreover, we show that dCRIF may function by interacting with, and stabilizing, the RNase III enzyme Dicer-2. Our results suggest that dCRIF may play an important role in regulating the RNAi pathway.
Requirement for CRIF1 in RNA interference and Dicer-2 stability
Creators: name: Jun Lim, Su
name: Scott, Anthony
name: Xiong, Xiao-Peng
name: Vahidpour, Shabnam
name: Karijolich, John
name: Guo, Dongdong
name: Pei, Shanshan
name: Yu, Yi-Tao
name: Zhou, Rui
name: X Li, Willis
Publisher: name: Taylor & Francis
Year: 2014
Subjects: subject: Virology
subject: Hematology
subject: Mental Health
subject: Developmental Biology
subject: Biological Sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: Earth and Environmental Sciences
subject: Molecular Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Cell Biology
subject: Microbiology
subject: Biochemistry
subject: Biophysics

description: RNA interference (RNAi) is a eukaryotic gene-silencing system. Although the biochemistry of RNAi is relatively well defined, how this pathway is regulated remains incompletely understood. To identify genes involved in regulating the RNAi pathway, we screened for genetic mutations in Drosophila that alter the efficiency of RNAi. We identified the Drosophila homolog of the mammalian CR6-interacting factor 1 (CRIF1), also known as growth arrest and DNA-damage-inducible 45-gamma interacting protein (Gadd45GIP1), as a potential new regulator of the RNAi pathway. Loss-of-function mutants of Drosophila CRIF1 (dCRIF) are deficient in RNAi-mediated target gene knock-down, in the biogenesis of small interfering RNA (siRNA) molecules, and in antiviral immunity. Moreover, we show that dCRIF may function by interacting with, and stabilizing, the RNase III enzyme Dicer-2. Our results suggest that dCRIF may play an important role in regulating the RNAi pathway.
title: Marc Girard
Creators: name: Girard, Marc (1936-....)
Publisher: name: NAKALA - https://nakala.fr (Huma-Num - CNRS)
publisherIdentifier: https://ror.org/04ces3204
publisherIdentifierScheme: ROR
Year: 2020

description: Autre destinataire : Marie-Paule Kieny. 1 fichier (1 page).
CO-CREATING EXERGAMES WITH PARKINSON'S DISEASE PATIENTS
Creators: name: Savvidis, Theodore
name: Konstantinidis, Evdokimos
name: Sofia, Dias
name: Zilidou, Vassiliki
name: Romanopoulou Evangelia
name: Hadjileontiadis Leontios
name: Bamidis Panagiotis
Publisher: name: Zenodo
Year: 2019

description: In this study, the Minimum Viable Product (MVP) model was applied with regards to “Measure-developlearn” circle along with its impact on the decision for the development tools and technologies. An example of designing exergames for Parkinson’s disease (PD) patients by considering their valuable feedback [1] and how this influenced low-level technical decisions (changing game engine from 2D Exergames to 3D) are thoroughly discussed.
title: 02_1712
Creators: name: Wesley Baldwin, John
name: Baldwin, John W.
Publisher: name: NAKALA - https://nakala.fr (Huma-Num - CNRS)
publisherIdentifier: https://ror.org/04ces3204
publisherIdentifierScheme: ROR
Year: 2018
Subjects: subject: LaMOP
subject: Laboratoire de Médiévistique Occidentale de Paris

description: L'ensemble de ces notes ont été crée par John Baldwin lors d'une de ses premières périodes de résidence à Paris. Ces documents comprennent tous les magistri qu’il avait pu rencontrer dans ses sources. Aux XIIème et XIIIème siècles, les universités n'existant pas encore, le titre de magister s'il évoque certainement une compétence n'est pas rattaché de façon systématique au passage par les écoles. De surcroît, les magistris sont le plus souvent désignés par leur seul prénom. La lecture de ces données est donc difficile et suppose un long travail pour tenter d'identifier un individu auquel on pourrait rattacher plusieurs mentions. John Baldwin avait donc renoncé à utiliser ces données pour donner un prolongement à sa magnifique étude sur les gradués d'université et leur emploi par les Capétiens et les Plantagenêts. Néanmoins, il a conservé ces données et elles sont potentiellement très utile dans le cadre de Studium, comme le sont toutes les mentions dans les volumes des Fasti Ecclesiae Gallicanae de chanoines portant ce titre de magister sans autres précisions. L'ampleur des dépouillements rend ces données très utiles et nous devons une grande reconnaissance à John Baldwin pour avoir pris la peine de faire photocopier ses fiches et de nous les avoir transmises amicalement.
Les données ainsi que les images des fiches sont accessibles sur le site Internet à l'adresse suivante : http://studium-baldwin.lamop.fr/.
Les transcriptions des fiches ont été effectuées par des étudiants de Paris 1 Panthéon - Sorbonne mais elles n'ont pas été corrigées par la suite.
description: Relation: https://lamop.hypotheses.org/
Technological diversity in Europe: empirical evidence from agri-food research projects
Creators: name: Muscio, Alessandro
name: Ciffolilli, Andrea
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: Science Policy

description: Technological diversity in Europe: empirical evidence from agri-food research projects. Regional Studies. Regional technological diversity is the capacity of regions to carry out research in multiple technology fields. Based on the analysis of 730 competitive research projects funded by the 7th European Framework Programme in agri-food, we show that the capacity to obtain funding and the regional degree of centrality in collaborative networks promote technological diversity. European programmes for research tend to encourage specialization in peripheral territories and technological diversity in central regions. This has challenging implications for a policy that wants to promote research excellence and, simultaneously, to reduce the wide regional variation in research and innovation performance across the European Union.
Electrochemically Reduced Carboxyl Graphene Modified Electrode for Simultaneous Determination of Guanine and Adenine
Creators: name: Xu, Juan
name: Li, Tianbao
name: Shen, Shaofei
name: Zhao, Lei
name: Ma, Chao
name: Mahmoud, Abd Elaal
name: Wang, Jinyi
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Physiology
subject: FOS: Biological sciences
subject: Biochemistry
subject: Biophysics
subject: Biological Sciences
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Biotechnology

description: An electrochemically reduced carboxyl graphene modified glassy carbon electrode (ERCGr/GCE) was prepared from a carboxyl graphene modified glassy carbon electrode (CGr/GCE) and employed for the simultaneous determination of guanine and adenine. The ERCGr/GCE showed an enhanced voltammetric response toward the oxidation of guanine and adenine compared with the CGr/GCE because the conductivity and electrochemical active surface area increased during the reduction process. The voltammetric peak current was linearly dependent on guanine and adenine concentration over the ranges of 0.5–10 and 2.5–50 µmol L−1, respectively. The detection limits were 0.15 µmol L−1 for guanine and 0.10 µmol L−1 for adenine in 50 µmol L−1 phosphate buffer at pH 6.86. Determination of guanine and adenine in thermally denatured herring sperm DNA showed that the ratio of guanine/adenine was 0.758 demonstrating practical application of the ERCGr/GCE.
Positioning Peanuts: Linus, Charlie, and the making of auteur comics magazines in Italy and France (1965-1975)
Creators: name: BUSI RIZZI, GIORGIO
name: Zanetti, Sara
Publisher: name: Zenodo
Year: 2024
Subjects: network analysis
comics
Linus
Charlie
media sociology
sociology of literature
cultural history

description: This academic poster was created for the DH Benelux 2024 conference. It proposes a preliminary survey of the early years of the auteur comics magazines Linus and Charlie.
Electrochemically Reduced Carboxyl Graphene Modified Electrode for Simultaneous Determination of Guanine and Adenine
Creators: name: Xu, Juan
name: Li, Tianbao
name: Shen, Shaofei
name: Zhao, Lei
name: Ma, Chao
name: Mahmoud, Abd Elaal
name: Wang, Jinyi
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Physiology
subject: FOS: Biological sciences
subject: Biochemistry
subject: Biophysics
subject: Biological Sciences
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Biotechnology

description: An electrochemically reduced carboxyl graphene modified glassy carbon electrode (ERCGr/GCE) was prepared from a carboxyl graphene modified glassy carbon electrode (CGr/GCE) and employed for the simultaneous determination of guanine and adenine. The ERCGr/GCE showed an enhanced voltammetric response towards the oxidation of guanine and adenine compared with the CGr/GCE because the conductivity and electrochemical active surface area increased during the reduction process. The voltammetric peak current was linearly dependent on guanine and adenine concentration over the ranges of 0.5–10 and 2.5–50 micromolars, respectively. The detection limits were 0.15 micromolar for guanine and 0.10 micromolar for adenine in 50 millimolars phosphate buffer at pH 6.86. Determination of guanine and adenine in thermally denatured herring sperm DNA showed that the ratio of guanine/adenine was 0.758 demonstrating practical application of the ERCGr/GCE.
DNA binding, cleavage and cytotoxicity of binuclear phenolate nickel(II) complexes
Creators: name: Li, Dong-Dong
name: Zhao, Xiu-Mei
name: Gu, Na
name: Zhi, Shuang
name: Zun-Wei Tao
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Biophysics
subject: Biochemistry
subject: Cell Biology
subject: Molecular Biology
subject: Pharmacology
subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Cancer

description: Three binuclear phenolate complexes, [Ni2(L1)2(OAc)](BPh4)·DMF (1), [Ni2(L2)2(OAc)](BPh4) (2) and [Ni2(L3)2(OAc)](OH)·3H2O (3), where L1 = 2-{[bis-(2-hydroxy-ethyl)-amino]-methyl}-4-methyl-phenol, L2 = 2-{[bis-(2-hydroxy-ethyl)-amino]-methyl}-4-methoxy-phenol and L3 = 2-{[bis-(2-hydroxy-ethyl)-amino]-methyl}-4-tert-butyl-phenol), have been synthesized. Single-crystal diffraction reveals that all the metal atoms are in a distorted octahedral geometry. The interactions of the complexes with calf thymus DNA (CT-DNA) have been investigated by UV-vis absorption, fluorescence emission and circular dichroism spectroscopy and viscosity measurements. Furthermore, DNA-cleavage mechanism shows that the complexes may be capable to promote DNA-cleavage through oxidative DNA-damage pathway, which is indicative of the involvement of hydroxyl radical, singlet oxygen or singlet oxygen-like entity in the cleavage process. Cytotoxicity studies on the Hela and MCF-7 cancer cell lines show that complexes 1-3 exhibit excellent activity toward the tested tumor cell lines with respect to the standard drug carboplatin, revealing that they have the potential to act as effective metal-based anticancer drugs.
Syntheses, crystal structures, electrochemical studies, and antioxidant activities of zinc(II) and copper(II) complexes with bis(2-benzimidazolyl) aniline derivatives
Creators: name: Wu, Huilu
name: Zhang, Jiawen
name: Zhang, Yanhui
name: Chen, Chengyong
name: Li, Zhen
name: Wu, Mingchang
name: Yang, Zaihui
Publisher: name: Taylor & Francis
Year: 2015
Subjects: Biological Sciences
Chemistry
Earth and Environmental Sciences
Cell Biology
Biochemistry
Biophysics

description: Two ligands, bis(benzimidazol-2-ylmethyl) aniline (bba) and bis(N-methyl-benzimidazol-2-ylmethyl) aniline (Mebba), and their transition metal complexes [Zn(bba)(Br)2]·2DMF (1) and [Cu(Mebba)(Br)2]·2DMF (2) have been synthesized and characterized by elemental analyses, molar conductivities, UV–vis spectra, IR spectra, NMR spectroscopy, and X-ray crystallography. The structure around Zn(II) can be described as distorted tetrahedral. Complex 2 can be described as distorted trigonal bipyramidal. Cyclic voltammograms of 2 indicate a quasireversible Cu2+/Cu+ couple. Additionally, the antioxidant activities of the free ligands and their complexes were determined by the superoxide and hydroxyl radical scavenging methods in vitro. Complexes 1 and 2 possess potent hydroxyl radical scavenging activity and better than standard antioxidants such as vitamin C and mannitol. Complex 2 possesses excellent superoxide radical activity.
Persistent response of Fanconi anemia haematopoietic stem and progenitor cells to oxidative stress
Creators: name: Yibo Li
name: Amarachintha, Surya
name: Wilson, Andrew F.
name: Li, Xue
name: Du, Wei
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Molecular Biology
subject: Physiology
subject: 69999 Biological Sciences not elsewhere classified
subject: Cancer
subject: 111714 Mental Health
subject: FOS: Health sciences
subject: Computational Biology

description: Oxidative stress is considered as an important pathogenic factor in many human diseases including Fanconi anemia (FA), an inherited bone marrow failure syndrome with extremely high risk of leukemic transformation. Members of the FA protein family are involved in DNA damage and other cellular stress responses. Loss of FA proteins renders cells hypersensitive to oxidative stress and cancer transformation. However, how FA cells respond to oxidative DNA damage remains unclear. By using an in vivo stress-response mouse strain expressing the Gadd45β-luciferase transgene, we show here that haematopoietic stem and progenitor cells (HSPCs) from mice deficient for the FA gene Fanca or Fancc persistently responded to oxidative stress. Mechanistically, we demonstrated that accumulation of unrepaired DNA damage, particularly in oxidative damage-sensitive genes, was responsible for the long-lasting response in FA HSPCs. Furthermore, genetic correction of Fanca deficiency almost completely abolished the persistent oxidative stress-induced G2/M arrest and DNA damage response in vivo. Our study suggests that FA pathway is an integral part of a versatile cellular mechanism by which HSPCs respond to oxidative stress.
Syntheses, crystal structures, electrochemical studies and antioxidant activities of zinc(II) and copper(II) complexes with bis(2-benzimidazolyl)aniline derivatives
Creators: name: Wu, Huilu
name: Zhang, Jiawen
name: Zhang, Yanhui
name: Chen, Chengyong
name: Li, Zhen
name: Wu, Mingchang
name: Yang, Zaihui
Publisher: name: Taylor & Francis
Year: 2015
Subjects: Biological Sciences
Chemistry
Earth and Environmental Sciences
Cell Biology
Biochemistry
Biophysics

description: Two ligands, bis(benzimidazol-2-ylmethyl)aniline (bba) and bis(N-methyl-benzimidazol-2ylmethyl)aniline (Mebba), and their transition metal complexes [Zn(bba)(Br)2]·2DMF (1) and [Cu(Mebba)(Br)2]·2DMF (2) have been synthesized and characterized by elemental analyses, molar conductivities, UV–Vis spectra, IR spectra, NMR spectroscopy and X-ray crystallography. The structure around Zn(II) can be described as distorted tetrahedral. Complex 2 can be described as distorted trigonal bipyramidal. Cyclic voltammograms of 2 indicate a quasireversible Cu2+/Cu+ couple. Additionally, the antioxidant activities of the free ligands and their complexes were determined by the superoxide and hydroxyl radical scavenging methods in vitro. Complexes 1 and 2 possess potent hydroxyl radical scavenging activity and better than standard antioxidants like vitamin C and mannitol. Complex 2 possesses excellent superoxide radical activity.
Exploring the reaction channels between arsine and the hydroxyl radical
Creators: name: Rommel B. Viana
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Genetics
subject: Molecular Biology
subject: Physiology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: Cancer
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: The aim of this study was to present the reaction mechanism channels between arsine (AsH3) and hydroxyl (OH) which was evaluated at CCSD(T)/CBS//CCSD/cc-pVTZ level. One potential channel is the hydrogen abstraction pathway (R1), leading to AsH2 and H2O products, which occurs due to the formation of an entrance complex (AsH3OH) followed by a 1,2-hydrogen shift pathway (involving the proton transfer from the arsine group to hydroxyls, with one leading to the products). Additional channels are accessed via H-elimination pathways of the entrance complexes, forming arsinous acid (AsH2OH; R2) and arsine oxide (AsH3O; R3). In this respect, R2 is the only exoergic route of the three exit channels, representing the major branching ratio at 200–1000 K and, after 2000 K, R1 increases gradually becoming the major route of this reaction. In contrast, even at 4000 K, R3 is a highly unfeasible pathway. Therefore, the information predicted here provides new insights into the neutral–neutral chemical reaction dynamics regarding the Group V hydrides. On the other side, the R2 pathway may have some potential to solve the arsine oxidation puzzle as a possible primary pathway to the arsenic-oxygen species formation.
Bioinspired study of energy and electron transfer in photovoltaic system
Creators: name: Md Moniruddin
name: Ilyassov, Baurzhan
name: Seliverstova, Evgeniya
name: Shabdan, Yerkin
name: Bakranov, Nurlan
name: Niyazbek Ibrayev
name: Nurxat Nuraje
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Biophysics
subject: Biochemistry
subject: Space Science
subject: 29999 Physical Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: Microbiology
subject: FOS: Biological sciences
subject: Physiology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified

description: This study focuses on understanding the fundamentals of energy transfer and electron transport in photovoltaic devices with uniquely designed nanostructures by analysing energy transfer in purple photosynthetic bacteria using dye-sensitised solar cell systems. Förster resonance energy transfer between the xanthene dye (donor of energy) and a new polymethine dye (acceptor of energy) was studied in dye-sensitised solar cells, which leads to a doubling of energy conversion efficiency in comparison to the cell with only the polymethine dye. The electron transport in the two different nanostructures of zinc oxide (nanorods and nanosheets) was investigated by spectroscopic methods (UV-vis spectrometer, time-resolved photoluminescence spectroscopy) and electrochemical potentiostat methods. The nanosheet structure of zinc oxide showed high short circuit current and long diffusion length. This fundamental study will lead to efficient artificial photosystem designs.
The synthesis, structure, and fluorescence properties of two heptanuclear coordination compounds containing trigonal prismatic [LnCu6(μ-OH)3(Gly)6Im6]6+ cations (Ln: Eu, Sm)
Creators: name: Pan, Lu
name: Gao, Xiao-Han
name: Lv, Xue-Chuan
name: Cao, Hui
name: Tan, Zhi-Cheng
name: Jiao, Yu-Juan
name: Geng, Ren-Yong
name: Luo, Guan-Hua
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Plant Biology
subject: FOS: Biological sciences
subject: Biological Sciences
subject: Chemistry
subject: Biotechnology
subject: Physics
subject: Biochemistry
subject: Biophysics

description: Two heptanuclear trigonal prismatic polyhedra, [EuCu6(μ-OH)3(Gly)6Im6](ClO4)6·3H2O and [SmCu6(μ-OH)3(Gly)6Im6](ClO4)6·3H2O were synthesized through self-assembly of Eu3+(Sm3+), Cu2+, glycine, and imidazole in aqueous solution and characterized by X-ray single crystal diffraction. Both complexes crystallize in the rhombohedral R3 space group (a = b = 15.8673 Å, c = 23.4038 Å, V = 5103.0(6) Å3, Z = 3) and (a = b = 15.8749 Å, c = 23.455 Å, V = 5119.1(8) Å3, Z = 3). In the clusters, Sm3+ and Eu3+ ions are situated in the center of a prism formed by six copper ions and coordinate to nine oxygens with a tricapped trigonal prismatic coordination polyhedron. Six glycinato ligands and six imidazole terminal ligands (each coordinates to one Cu2+) construct the cluster. The fluorescent excitation and emission spectra showed that the complexes have strong ligand-based fluorescence.
Agribusiness Firms and Rural Dairy Development. A Case of FrieslandCampina Dairy Development Programme in Nigeria
Creators: name: Amolegbe, Khadijat Busola
name: Adewumi, Matthew Olaniyi
name: Amolegbe, Khadijat Busola
name: Adewumi, Matthew Olaniyi
Publisher: name: Unknown
Year: 2022
Subjects: subject: Agribusiness
subject: Agricultural and Food Policy
subject: Community/Rural/Urban Development
subject: dairy
subject: Nigeria
subject: agribusiness firms
subject: FrieslandCampina
subject: rural farmers

description: Rural development interventions funded by private agribusiness firms may positively or negatively affect rural farmers' welfare. A positive effect is that such interventions may provide farmers with market access. The negative effect could be that such firms may be solely motivated by profit and may exploit the farmers. In this paper, we explore the role of FrieslandCampina Dairy Development Programme, a multinational firm with headquarters in Europe, in improving the welfare of rural dairy farmers in Nigeria. We use a two-wave panel survey of 122 programme participants and 95 non-participants. We focus on two outcome measures – annual dairy income and daily milk yield - and use a pooled ordinary least squares method to understand the programme effect. We also explore the mechanism of effect by assessing the programme effects on farmers' sustainable dairy management practices using a negative binomial regression method. Our results suggest that the programme has positive welfare effects on farmers. We attribute these effects to farmers' access to reliable markets offered by the programme and the informal business arrangement between the farmers and the agribusiness firm. Potential policy implications include that governments should encourage other private agribusiness firms to set up similar development programmes.
The synthesis, structure, and fluorescence properties of two heptanuclear coordination compounds containing trigonal prismatic [LnCu6(μ-OH)3(Gly)6Im6]6+ cations (Ln: Eu, Sm)
Creators: name: Pan, Lu
name: Gao, Xiao-Han
name: Lv, Xue-Chuan
name: Cao, Hui
name: Tan, Zhi-Cheng
name: Jiao, Yu-Juan
name: Geng, Ren-Yong
name: Luo, Guan-Hua
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Plant Biology
subject: FOS: Biological sciences
subject: Biological Sciences
subject: Chemistry
subject: Biotechnology
subject: Physics
subject: Biochemistry
subject: Biophysics

description: Two heptanuclear trigonal prismatic polyhedra, [EuCu6(μ-OH)3(Gly)6Im6](ClO4)6∙3H2O and [SmCu6(μ-OH)3(Gly)6Im6](ClO4)6∙3H2O, were synthesized through self-assembly of Eu3+(Sm3+), Cu2+, glycine and imidazole in aqueous solution and characterized by X-ray single crystal diffraction. Both complexes crystallize in the rhombohedral R3 space group (a = b = 15.8673 Å, c = 23.4038 Å, V = 5103.0(6) Å3, Z = 3) and (a = b = 15.8749 Å, c = 23.455 Å, V = 5119.1(8) Å3, Z = 3). In the clusters, Sm3+ and Eu3+ ions are situated in the center of a prism formed by six copper ions and coordinate to nine oxygens with a tricapped trigonal prismatic coordination polyhedron. Six glycinato ligands and six imidazole terminal ligands (each coordinates to one Cu2+) construct the cluster. The fluorescent excitation and emission spectra showed that the complexes have strong ligand-based fluorescence.
title: 'Surfing the edge of chaos'
title: an ethnography of police joint working
titleType: Subtitle
Creators: name: Youansamouth, Lindsay
Publisher: name: Lancaster University
Year: 2020
Subjects: Policing
Multi-agency
Ethnography
Organisational cultures
Vulnerability
Law enforcement

description: Police joint working is a messy area of enquiry, which has thus far been somewhat compartmentalised in previous research. This thesis reflects the complexities of police joint working amidst an era of austerity. It advances complex adaptive systems (CAS) as a theoretical construct, through which to study and comprehend the process of joint working. The principal aim is to provide a deeper and richer understanding of joint working, by drawing primarily on observations and experiences of warranted police officers and civilian police staff. The more specific objectives are to identify the utility of police joint working as a solution to social problems; investigate how joint work is accomplished using an ethnographic approach; contribute to understanding how joint working challenges might be approach differently. Through the application of CAS, a holistic and contextualised account of joint working is provided. In this respect, the thesis differs from previous atheoretical studies and literature employing a ‘communities of practice’ (CoP) approach. Whilst acknowledging the relevance of shared cultural factors, this thesis seeks to shift attention to wider – personal and structural – contextual influences that give rise to the ‘punctuated’ progress in working together, generally overlooked in existing joint working theory. Ethnographic methods were applied as a key to unlock the intricacies and diversity of joint working experiences – both front- and back-stage – as the researcher was immersed in a large police force in England for 18-months. Fieldwork insights exposed the fragmented, unpredictable and interconnected ways in which joint working changes over time. Thus, networked policing was revealed as messy, evolving and seemingly out of control. The implications section stresses the importance of developing a formalised supervision model as a source of stability through, which employees can find ways to navigate change ‘churn’ and ‘surf the chaos’.
Persistent response of Fanconi anemia hematopoietic stem and progenitor cells to oxidative stress
Creators: name: Yibo Li
name: Amarachintha, Surya
name: Wilson, Andrew F.
name: Li, Xue
name: Du, Wei
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Molecular Biology
subject: Physiology
subject: 69999 Biological Sciences not elsewhere classified
subject: 111714 Mental Health
subject: FOS: Health sciences
subject: Computational Biology

description: Oxidative stress is considered as an important pathogenic factor in many human diseases including Fanconi anemia (FA), an inherited bone marrow failure syndrome with extremely high risk of leukemic transformation. Members of the FA protein family are involved in DNA damage and other cellular stress responses. Loss of FA proteins renders cells hypersensitive to oxidative stress and cancer transformation. However, how FA cells respond to oxidative DNA damage remains unclear. By employing an in vivo stress-response mouse strain expressing the Gadd45β-luciferase transgene, we show here that hematopoietic stem and progenitor cells (HSPCs) from mice deficient for the FA gene Fanca or Fancc persistently responded to oxidative stress. Mechanistically, we demonstrated that accumulation of unrepaired DNA damage, particularly in oxidative damage-sensitive genes, was responsible for the long-lasting response in FA HSPCs. Furthermore, genetic correction of Fanca deficiency almost completely abolished the persistent oxidative stress-induced G2/M arrest and DNA damage response in vivo. Our study suggests that FA pathway is an integral part of a versatile cellular mechanism by which HSPCs respond to oxidative stress.
title: Social justice is the best therapy
title: exploring lived-experiences of welfare reform in the United Kingdom
titleType: Subtitle
Creators: name: Thompson, Charlotte
Publisher: name: Lancaster University
Year: 2020

description: This thesis consists of a literature review, a research paper and a critical appraisal. The focus of this thesis is to understand the impact of welfare reform and austerity on individuals, with particular focus on mental wellbeing. In attempt to explore the impact of stigma on individuals who claim benefits a qualitative metasynthesis of 18 papers was completed. Findings demonstrated that individuals experienced multiple levels of benefit stigma, that stigma had social and emotional impact, individuals adopted various strategies to manage their stigmatised identity and that benefits stigma created an atmosphere of distrust and suspicion within communities. The research paper specifically explored the impact of applying for Personal Independence Payment (PIP) on mental wellbeing. PIP is a form of disability benefit introduced under the Welfare Reform Act. Thematic analysis of qualitative data yielded from 16 interviews led to the development of six related themes: 1) PIP is ‘gruelling’ throughout: designed to frustrate?; 2) ‘Treated like a criminal’ - a dehumanising, criminalising process; 3) ‘It’s life threatening for some people’ - impact of PIP on mental health; 4) ‘It feels like they’re trying to cull the weak’- lacking a sense of belonging and worth in society; 5) ‘They need to make improvements’ –how PIP could be improved. These findings captured the distressing nature of the PIP process. Finally, the critical appraisal provides a reflective stance on a central issue underlying many of the recommendations made within the literature review and empirical paper- the role of Clinical Psychology in politics and social justice. It is argued that psychological thinking can offer unique insight into some of the most pressing issues society faces. Therefore we have a responsibility to contribute our research findings and our theoretical understandings to public, social and political debates.
Proyecto Ley MinCyT #FPC
Creators: name: Por El Conocimiento Frente
Publisher: name: figshare
Year: 2017
Subjects: subject: 160699 Political Science not elsewhere classified
subject: FOS: Political science
subject: 160511 Research, Science and Technology Policy

description: Indicaciones al proyecto de Ley que crea el Ministerio de Ciencia y Tecnología