cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Мониторинг ферментативной активности уреазы в серых лесных почвах Верхневолжья
Creators: name: С. И. ЗИНЧЕНКО
name: М. К. ЗИНЧЕНКО
Publisher: name: Земледелие
Year: 2020
Subjects: ферментативная активность уреазы
агроландшафты
серая лесная почва
фон интенсификации
транс- формация мочевины

description: Исследования проводили с целью мо- ниторинга активности уреазы в пахотной серой лесной слабооподзоленной средне- суглинистой почве. Работу осуществляли в 2012–2018 гг. во Владимирской области в многофакторном полевом опыте на сле- дующих фонах применения минеральных и органоминеральных удобрений: низкий – навоз 40 т/га; интенсивный – N100P80K160 + навоз 40 т/га; интенсивный минеральный – N350P220K390; интенсивный органоминераль- ный – N310P150K310 + навоз 60 т/га; высоко- интенсивный минеральный – N480P280K575; высокоинтенсивный органоминеральный – N430P160K360 + навоз 80 т/га (количество удо- брений приведено за ротацию 6-польного севооборота). Содержание гумуса в па- хотном слое (0…20 см) почвы составляло 3,9…4,2 %, подвижного фосфора – 100… 150 мг/кг, обменного калия –100…120 мг/ кг, pHKCl – 5,9…6,1 ед. Природный аналог почвы отбирали на сопредельном с опы- том участке многолетней залежи. Выявлен устойчивый тренд увеличения активности уреазы в конце июня – июле на всех фонах интенсификации. На органоминеральных фонах наблюдали достоверно более высо- кую уреазную активность, по сравнению с минеральными: в среднем соответственно 0,22…0,30 и 0,15…0,18 мг N-NН4/г почвы за 4 часа (НСР05 = 0,08). Выявлена тесная положительная связь между активностью уреазы и численностью бактерий, транс- формирующих азот органических остатков (аминогетеротрофов) – r = 0,85. Фермен- тативная трансформация мочевины более активно протекала на органоминеральных фонах с плоскорезной обработкой. В этом варианте при внесении 60 т/га навоза она была в среднем на 26 % выше, чем по отвальной вспашке. Самую высокую ско- рость разложения мочевины при оценке активности уреазы экспресс-методом Т. В. Аристовской отмечали в почве залежи и на высокоинтенсивном органоминераль- ном фоне. Наименьшей она была на низком и высокоинтенсивном минеральном фоне. Результаты мониторинга фермента могут быть использованы при агроэкологической диагностике состояния агроландшафтов.
Additional file 2 of Impacts of attending an inclusive STEM high school: meta-analytic estimates from five studies
Creators: name: Means, Barbara
name: Wang, Haiwen
name: Wei, Xin
name: Young, Viki
name: Iwatani, Emi
Publisher: name: figshare
Year: 2021
Subjects: subject: Medicine
subject: Environmental Sciences not elsewhere classified
subject: Sociology
subject: FOS: Sociology
subject: Biological Sciences not elsewhere classified
subject: Science Policy

description: Additional file 2: Appendix A-2. Outcome Measures Used in the Meta-Analysis
Additional file 2 of SHOOT: phylogenetic gene search and ortholog inference
Creators: name: Emms, David Mark
name: Kelly, Steven
Publisher: name: figshare
Year: 2022
Subjects: subject: Biochemistry
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Evolutionary Biology
subject: 69999 Biological Sciences not elsewhere classified
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: Cancer
subject: Plant Biology

description: Additional file 2. Review history.
Additional file 1 of Abundant small RNAs in the reproductive tissues and eggs of the honey bee, Apis mellifera
Creators: name: Watson, Owen T.
name: Buchmann, Gabriele
name: Young, Paul
name: Lo, Kitty
name: Remnant, Emily J.
name: Yagound, Boris
name: Shambrook, Mitch
name: Hill, Andrew F.
name: Oldroyd, Benjamin P.
name: Ashe, Alyson
Publisher: name: figshare
Year: 2022
Subjects: subject: Genetics
subject: FOS: Biological sciences

description: Additional file 1.
Биологическая активность и питательный режим почвы при различных приемах обработки под однолетние травы
Creators: name: Л. В. ГАРМАШОВА
name: М. П. КРЯЧКОВА
name: В. Н. ГОВОРОВ
name: И. М. КОРНИЛОВ
name: Н. А. НУЖНАЯ
name: В. М. ГАРМАШОВ
name: В. И. ТУРУСОВ
Publisher: name: Земледелие
Year: 2020
Subjects: обработка почвы
биологическая активность почвы
питательный режим
однолетние травы
урожайность

description: Исследования проводили с целью выявления эффективных приемов об- работки почвы под однолетние травы в Воронежской области в 2018–2020 гг. Схема опыта предполагала изучение следующих вариантов: прием обработки почвы (фактор А) – ежегодная вспашка на 20…22 см; ежегодная вспашка на 14…16 см; ежегодная безотвальная обработка на 14…16 см; ежегодная поверхностная обработка на 6…8 см; нулевая; удобрения (фактор В) – без удобрений, N60Р60К60. Почва опытного участка – чернозем обыкновенный среднегумусный тяже- лосуглинистый с содержанием гумуса в слое 0…30 см 6,48 %, Р2О5 – 100…140 мг/кг, К2О – 50…90 мг/кг, рН солевой вы- тяжки – 6,99 ед. Изменение свойств по- чвы сравнивали с залежью. Применение мелкой безотвальной, поверхностной и нулевой обработок приводит к снижению биологической активности почвы и обе- спеченности почвы нитратным азотом, по сравнению с вспашкой на глубину 20… 22 см. Урожайность однолетних трав (го- рох + овес) на зеленый корм имела четкую тенденцию к снижению при минимали- зации обработки почвы, статистически доказуемую при нулевой обработке. В почвенно-климатических условиях юго- востока ЦЧР при выращивании однолет- них трав (горох + овес) на зеленый корм возможно уменьшение глубины обработки почвы независимо от способа до 14…16 см и даже применение поверхностной обработки на глубину 6…8 см при тенден- ции к снижению урожайности. Нулевая обработка почвы под однолетние травы приводит к существенному (в 1,5…1,8 раза) уменьшению сбора продукции на удобренном и неудобренном фонах.
Предпосевная обработка почвы при разных способах посева зерновых культур
Creators: name: Н. К. ПЕРВУШИНА
name: М. В. ГУЛЯЕВ
name: Л. И. ПЕТРОВА
name: Ю. И. МИТРОФАНОВ
Publisher: name: Земледелие
Year: 2020
Subjects: гребнистый посев
культивация
комбинированные агрегаты
полевая всхожесть
сохранность
струк- тура урожая
урожайность

description: Исследования проводили с целью изучения эффективности приемов пред- посевной обработки почвы при разных способах посева зерновых культур. Ра- боту осуществляли в 1991–1994 гг. и 2016–2019 гг. в условиях Тверской области на дерново-подзолистой хорошо окуль- туренной легкосуглинистой глееватой почве, осушаемой закрытым дренажом. На хорошо дренированных почвах легко- го гранулометрического состава лучший вариант предпосевной обработки при использовании обычных зерновых сеялок СЗ-3,6 с дисковыми сошниками – культи- вация в один след на глубину 4…6 см с по- следующей обработкой комбинированным агрегатом типа РВК-3,6. Его применение обеспечивает повышение полевой всхо- жести семян овса на 5,9 %, ячменя – на 3,0 %, озимой ржи – на 7,6 % и увеличение урожайности – соответственно на 0,24, 0,32 и 0,27…0,36 т/га. При гребнистом ленточно-разбросном способе посева с локальным уплотнением почвы примене- ние РВК-3,6 влияния на урожайность зер- новых культур не оказывает. Сбор зерна овса в варианте с двойной культивацией составлял 3,78 т/га, с РВК-3,6 – 3,71 т/га, ячменя – соответственно 3,93 и 3,85 т/га, ржи – 5,71 и 5,79 т/га. Преимущество гребнистого способа посева обусловлено оптимизацией площади питания растений и улучшением агрофизических условий в посевном слое почвы. Прибавки урожая овса при гребнистом способе посева, по сравнению с рядовым, составляли 0,13…0,37 т/га, ячменя – 0,23…0,55 т/га, озимой ржи – 0,58…0,85 т/га. Улучшенный гребнистый ленточно-разбросной способ посева (высота гребней 6…8 см, ширина лент – 13…15 см) рекомендуется для вы- ращивания овса, ячменя и озимой ржи в условиях повышенного увлажнения почвы, прежде всего в северо-западной части Нечерноземной зоны РФ.
Агробиологические особенности нового сорта клевера лугового Таежник
Creators: name: И. В. ЗОБНИНА
name: О. Б. БАТАКОВА
name: В. А. КОРЕЛИНА
Publisher: name: Земледелие
Year: 2020
Subjects: клевер луговой (Trifolium pretense)
селекция
отбор
кор- мовая и семенная продуктивность
зимо- стойкость
пластичность

description: Исследования проводили с целью соз- дания нового зимостойкого сорта клевера лугового с высокой потенциальной кормо- вой и семенной продуктивностью, а также устойчивостью к основным болезням и стрессам в условиях Европейского Севера Российской Федерации. Работу осущест- вляли в 2001–2016 гг. В ходе селекционной работы методом свободно-ограниченного переопыления при направленном подборе родительских форм создан сорт Таеж- ник, хорошо адаптированный к климату и почве Северо-Западного региона РФ. Селекционный номер СГП-12/01, выде- ленный в конкурсном сортоиспытании в 2014–2016 гг. и показавший преимущество по комплексу хозяйственно биологических признаков над стандартом Нива, был пере- дан в 2016 г. в Государственное сортоиспы- тание как сорт Таежник. Сорт диплоидный, среднеспелый, одноукосный. Это сложно- гибридная популяция, созданная методом поликросса, на основе дикорастущей формы из Алтайского края и местных по- пуляций Северного региона. Период от начала весеннего отрастания до первого укоса в среднем составляет 66 дней, до со- зревания семян – 110 дней. Зимостойкость высокая – 95…98 %. Средняя урожайность зеленой массы по результатам конкурсно- го сортоиспытания 38,0 т/га, что больше стандарта Нива на 4,93 т/га. Потенциал продуктивности сорта составляет 11,0 т/ га сухого вещества. Содержание в сухом веществе сырого протеина 14,9 %, сырой клетчатки – 24,4 % (на уровне стандарта Нива). Антракнозом и корневыми гнилями поражается в средней степени. В 2020 г. сорт Таежник внесен в Государственный реестр селекционных достижений, до- пущенных к использованию по Северо- Западному региону.
Осеннее внесение метрибузина для защиты озимой пшеницы от двудольных и злаковых сорных растений
Creators: name: В. И. ДОЛЖЕНКО
name: А. С. ГОЛУБЕВ
Publisher: name: Земледелие
Year: 2020
Subjects: пшеница озимая (Triticum aestivum L.)
гербициды
сорняки
метрибузин
метлица обыкновенная

description: Полевые мелкоделяночные опыты по осеннему использованию 138 и 210 г/га метрибузина (0,23 и 0,35 л/га – по количеству использующегося препарата Лазурит Ультра, СК) на посевах озимой пшеницы были проведены в течение двух вегетационных сезонов (2017–2018 и 2018–2019 гг.) в трех различных климати- ческих зонах Российской Федерации (Вол- гоградская область, Рязанская область и Краснодарский край). Опыты закладывали согласно «Методическим указаниям по ре- гистрационным испытаниям гербицидов в сельском хозяйстве» (2013). Учеты сорных растений проводили количественно- весовым методом. Урожайность учиты- вали вручную, методом пробных снопов с площади 1 м2 на каждой опытной делянке. Биологическая и хозяйственная эффек- тивность изучаемого гербицида в целом находилась на уровне эталона Морион, СК (500 г/л изопротурона + 100 г/л дифлюфе- никана) в нормах 0,75 и 1,0 л/га. Снижение количества метлицы обыкновенной под действием 210 г/га метрибузина состав- ляло не менее 90 %. Против дескурайнии Софии, пастушьей сумки обыкновенной, ярутки полевой, подмаренника цепкого, трехреберника непахучего и фиалки полевой эффективность препарата со- ставляла 80…100 %. Гербицид также оказывал значимое влияние на растения сокирок великолепных и лисохвоста мы- шехвостникового. Урожайность озимой пшеницы без обработки гербицидами в осенний период в зависимости от сорта и погодных условий варьировала от 2,03 т/ га (Волгоградская область, вегетационный период 2017–2018 гг.) до 5,39 т/га (Крас- нодарский край, вегетационный период 2018–2019 гг.). Устранение конкуренции со стороны сорных растений после осен- него внесения метрибузина способство- вало достоверному сохранению урожая (от 0,22 до 0,42 т/га).
Additional file 1 of Recombinant adenovirus expressing vesicular stomatitis virus G proteins induce both humoral and cell-mediated immune responses in mice and goats
Creators: name: Xue, Xiaojuan
name: Yu, Zhaorong
name: Jin, Hongyan
name: Liang, Lin
name: Li, Jiayang
name: Li, Xiaolu
name: Wang, Yong
name: Cui, Shangjin
name: Li, Gang
Publisher: name: figshare
Year: 2021
Subjects: subject: Immunology
subject: FOS: Clinical medicine

description: Additional file 1.
Новый фунгицид Протазокс в защите озимого ячменя от пятнистостей листьев в условиях Краснодарского края
Creators: name: Г. В. ВОЛКОВА
name: Ю. С. КИМ
name: А. В. ДАНИЛОВА
Publisher: name: Земледелие
Year: 2020
Subjects: ячмень (Hordeum vulgare L.)
сетчатая пятнистость (Pyrenophora teres)
темно-бурая пятнистость (Bipolaris sorokiniana)
фунгициды
эффективность
защита растений

description: Исследования проводили с целью из- учения биологической и хозяйственной эффективности нового трехкомпонентного контактно-системного фунгицида Протазокс, КС против пятнистостей озимого ячменя в условиях Краснодарского края для совер- шенствования агротехники возделывании культуры. Работу выполняли в Краснодар- ском крае в вегетационные сезоны 2017/18 и 2018/19 гг. Почва экспериментального участка типичная для центральной зоны Северо-Кавказского региона – чернозем вы- щелоченный. В опыте выращивали озимый ячмень сорта Рубеж. Обработку посевов экспериментальным препаратом проводили однократно в фазе выхода флагового листа в нормах 0,5 л/га, 0,75 л/га и 1,0 л/га; эталоном служил Амистар Трио, КЭ, 1,0 л/га, контроль – без обработки. Учёт развития болезней вели в динамике по шкалам, предложенным для пятнистостей листьев. Расчет биологической и хозяйственной эффективности осущест- вляли по общепринятым методикам. Био- логическая эффективность Протазокс, КС против сетчатой пятнистости варьировала от 70,7 % до 81,5 %, против темно-бурой пятни- стости – от 51,8 % до 64,8 %, что в вариантах с нормами 0,75 л/га и 1,0 л/га сопоставимо с эталоном Амистар Трио, КЭ (76,8 % и 60,6 % соответственно). Обработка Протазокс, КС позволила сохранить от 0,21 т/га до 0,53 т/га урожайности культуры (от 3,3 % до 8,4 % к контролю). При нормах использования 0,75 л/га и 1,0 л/га сохранность была на уровне эталона (0,52 т/га, или 8,3 %). Наибольший эффект обеспечивает опрыскивание новым фунгицидом в норме 1,0 л/га.
Additional file 1 of Using virtual reality in lumbar puncture training improves students learning experience
Creators: name: Vrillon, Agathe
name: Gonzales-Marabal, Laurent
name: Ceccaldi, Pierre-François
name: Plaisance, Patrick
name: Desrentes, Eric
name: Paquet, Claire
name: Dumurgier, Julien
Publisher: name: figshare
Year: 2022
Subjects: subject: Artificial Intelligence and Image Processing
subject: FOS: Computer and information sciences

description: Additional file 1.
Additional file 1 of Dysbiosis of vaginal microbiota associated with persistent high-risk human papilloma virus infection
Creators: name: Mei, Ling
name: Wang, Tao
name: Chen, Yueyue
name: Wei, Dongmei
name: Zhang, Yueting
name: Cui, Tao
name: Meng, Jian
name: Zhang, Xiaoli
name: Liu, Yuqing
name: Ding, Lisha
name: Niu, Xiaoyu
Publisher: name: figshare
Year: 2022
Subjects: subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Ecology
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Science Policy
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Virology

description: Additional file 1. Rarefaction curves. The horizontal axis indicates the sampling depth, and the vertical axis indicates the index. All the curves become flat as the sampling depth increases, indicating that the high sampling coverage was achieved in all samples.
Brain-targeted delivery of PEGylated nano-bacitracin A against Penicillin-sensitive and -resistant Pneumococcal meningitis: formulated with RVG29 and Pluronic® P85 unimers
Creators: name: Hong, Wei
name: Zhang, Zehui
name: Liu, Lipeng
name: Zhao, Yining
name: Zhang, Dexian
name: Liu, Mingchun
Publisher: name: Taylor & Francis
Year: 2018
Subjects: subject: Space Science
subject: Medicine
subject: Molecular Biology
subject: Neuroscience
subject: Pharmacology
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Hematology
subject: Infectious Diseases
subject: FOS: Health sciences

description: Pneumococcal meningitis (PM), caused by Streptococcus pneumonia, remains a high-burden disease in developing countries. Antibiotic therapy has been limited due to the inefficiency of drug transport across the blood-brain barrier (BBB) and the emergence of drug-resistant strains. In our preliminary study, PEGylated nano-self-assemblies of bacitracin A (PEGylated Nano-BA12K) demonstrated a strong antibacterial potency against S. pneumonia. In this study, the potential application of this micelle for the treatment of both Penicillin-sensitive and -resistant PM was studied. To address BBB-targeting and -crossing issues, PEGylated Nano-BA12K was formulated with a specific brain-targeting peptide (rabies virus glycopeptide-29, RVG29) and a P-glycoprotein inhibitor (Pluronic® P85 unimers) to construct a mixed micellar system (RVG29-Nano-BAP85). RVG29-Nano-BAP85 demonstrated a strong antibacterial potency against 13 clinical isolates of S. pneumonia, even higher than that of Penicillin G, a conventional anti-PM agent. RVG29-Nano-BAP85 had more cellular uptake in brain capillary endothelial cells (BCECs) and higher BBB-crossing efficiency than single formulated Nano-BAs as shown in an in vitro BBB model. The enhanced BBB-permeability was attributed to the synergetic effect of RVG29 and P85 unimers through receptor-mediated transcytosis, exhaustion of ATP, and reduction in membrane microviscosity. In vivo results further demonstrated that RVG29-Nano-BAP85 was able to accumulate in brain parenchyma as confirmed by in vivo optical imaging. In addition, RVG29-Nano-BAP85 exhibited high therapeutic efficiencies in both Penicillin-sensitive and -resistant PM mouse models with negligible systemic toxicity. Collectively, RVG29-Nano-BAP85 could effectively overcome BBB barriers and suppressed the growth of both drug-sensitive and -resistant S. pneumonia in the brain tissues, which demonstrated its potential for the treatment of PM.
Understanding Participating Site (pSite) Requirements for the National Covid Cohort Collaborative (N3C)
Creators: name: Chute, Chris
name: N3C Consortium
Publisher: name: Zenodo
Year: 2020
Subjects: IRB
SmartIRB
N3C
Clinical Data Sharing

description: The National COVID Cohort Collaborative (N3C) opted to submit a single IRB at one of the organizing sites (Johns Hopkins University) to minimize the burden of participation for each site ("pSite") contributing clinical data to the N3C Secure Data Enclave. The N3C community collaboratively contributed to the core protocol. However, respecting site autonomy, the central IRB was not required; each site could opt to submit the core protocol to their own local IRB. This presentation summarizes the requirements for each pSite. The most recent version of this document can be referenced as https://doi.org/10.5281/zenodo.4010868. For more information about N3C visit covid.cd2h.org
title: Sehbo – der Laubsägebock
title: Didaktische Hinweise zur Fertigung des Laubsägebocks Sehbo
titleType: Subtitle
Creators: name: Penning, Isabelle
name: Gulich, Riccardo
name: Glugla, Markus
Publisher: name: Universität Potsdam
Year: 2024
Subjects: subject: 370 Bildung und Erziehung

description: Lehrkräfte sind mit Blick auf ihre heterogenen Lerngruppen im Technikunterricht darauf angewiesen, einen differenzierten Unterricht zu gestalten, der personelle, organisatorische und sachliche Ressourcen miteinbezieht. Bei Fertigungsaufgaben bietet sich daher der Einsatz von Vorrichtungen an, über die auf die unterschiedlichen Lernvoraussetzungen von Schüler:innen reagiert werden kann. So lassen sich die Anforderungen von Fertigungsaufgaben gezielt variieren. 
Die vorliegende Publikation beschreibt den Sägebock „Sehbo“. Dieser ist eine Erweiterung einer klassischen Hilfsvorrichtung für die Arbeit mit einer Laubsäge, da sich die Arbeitshöhe individuell an die Körpergröße anpassen lässt. Dadurch wird eine ergonomische Arbeitshaltung ermöglicht, was insbesondere bei längeren Arbeitsphasen wichtig ist. Der lange Stiel des Sägebocks ermöglicht eine stufenlose Einstellung von Arbeitshöhen zwischen 100 cm und 120 cm durch Einspannen in der Werkbank. Zusätzlich zur Standardversion kann eine Einspannhilfe zum Wechsel des Sägeblattes der Laubsäge am Ende des Sägekopfes und einen Stiel mit Tischbefestigung für den Einsatz an Tischen ohne Spannzangen optional ergänzt werden.
Die Dokumentationen umfasst neben den Fertigungsunterlagen kleinschrittige Bauanleitungen, die ggf. auch gemeinsam mit Schüler:innen umgesetzt werden können. Zudem enthalten sind beispielhafte Kostenrechnungen sowie Schablonen, die zur Erleichterung des Fertigungsprozesses verwendet werden können. Des Weiteren wird durch vereinzelt beigefügte interaktive technische Zeichnungen die Adaption der Vorrichtungen ermöglicht.
Additional file 1 of Retirement as a predictor of physical functioning trajectories among older businessmen
Creators: name: Haapanen, Markus J.
name: Strandberg, Timo E.
name: Törmäkangas, Timo
name: von Bonsdorff, Monika E.
name: Strandberg, Arto Y.
name: von Bonsdorff, Mikaela B.
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Physiology
subject: FOS: Biological sciences
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Sociology
subject: FOS: Sociology
subject: Immunology
subject: FOS: Clinical medicine
subject: Developmental Biology
subject: Cancer

description: Additional file 1: Supplementary Table 1. Model Fit Statistics, Group Sizes and Average Latent Class Probabilities for Most Likely Class Membership. Figures. Reproduced with permission from [15]. Supplementary Fig. 1. Individual observations belonging to each of the five identified physical functioning trajectories. Reproduced with permission from [15].
Syntheses, crystal structures, optical and antibacterial properties of two organic-inorganic hybrids containing pyridinium-substituted triphenylphosphonium and tetrachlorocobaltate/copperate(II)
Creators: name: Wang, Zong
name: Chen, Ke-Lai
name: Li, Yu-Ting
name: Huang, Lin
name: Xu, Peng
name: Jiang, Xin-Yi
name: Ni, Chun-Lin
name: Li, Yan-Xian
name: Liang, Jin-Ying
name: Zhang, Yao-Mou
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Biophysics
subject: Biochemistry
subject: Physical Sciences not elsewhere classified
subject: Microbiology
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Pharmacology
subject: Biotechnology
subject: Environmental Sciences not elsewhere classified
subject: Chemical Sciences not elsewhere classified
subject: Ecology

description: In this article, we present the syntheses, crystal structures, vibrational spectra, optical and antibacterial properties of two new organic-inorganic hybrids, [Py(CH2)3TPP][CoCl4]·H2O (1) and [Py(CH2)3TPP][CuCl4] (2), where [Py(CH2)3TPP]2+ was 1-(1′-pyridyl-propyl)-3-triphenyl phosphonium. Two hybrids were crystallized in the monoclinic space group P21/n. The hybrids consisted of a [Py(CH2)3TPP]2+ cation and a tetrahedral [CoCl4]2−/[CuCl4]2− anion, where the crystal structure was stabilized by C−H···Cl, O−H···Cl, and C−H···O hydrogen bonds due to the presence of a water molecule in 1, and the crystal structure of 2 was stabilized by C−H···Cl hydrogen bonds. Experimental vibrational bands (IR and Raman) have been discussed and assigned. The intermolecular energy, the energy gap between HOMO and LUMO orbitals, as well as the first hyperpolarizabilities were predicted based on DFT calculations. The low energy gaps (1.26 eV for 1 and 1.20 eV for 2) were responsible for the charge transfer interactions that eventually occur between the cation and anion. The first hyperpolarizabilities (βtot) of 1 and 2 were 82 and 237 times as high as that of the standard KDP crystal, respectively, indicating that the as-prepared hybrids were excellent materials for NLO applications. Furthermore, it was found that both salts exhibit good bactericidal activity against Escherichia coli and Staphylococcus aureus.
ABC News/Washington Post Poll: Trump/2020 Democratic Presidential Nomination/Iran Drone Strike
Creators: name: ABC News
name: Washington Post
Publisher: name: Roper Center for Public Opinion Research
Year: 2020

description: President Donald Trump job performance (4); 2020 Democratic presidential nomination (6); characteristics of Democratic presidential candidates (5); Trump vs. Democratic candidates for President (6); enthusiasm for/against Trump (2); Trump impeachment investigation (1); Trump trial by Senate (4); Trump-ordered drone strike against Iran military leader (5); worried about maintaining current standard of living (1); registered to vote (1); voted in 2016 presidential election (2).
Additional file 1 of Verbal fluency as a quick and simple tool to help in deciding when to refer patients with a possible brain tumour
Creators: name: Zienius, Karolis
name: Ozawa, Mio
name: Hamilton, Willie
name: Hollingworth, Will
name: Weller, David
name: Porteous, Lorna
name: Ben-Shlomo, Yoav
name: Grant, Robin
name: Brennan, Paul M.
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Cell Biology
subject: Neuroscience
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Cancer
subject: Science Policy
subject: 111714 Mental Health
subject: FOS: Health sciences

description: Additional file 1: Supplementary Table 1. ANCOVA Model tables for each outcome verbal fluency outcome measure. Supplementary Table 2. Sensitivity and Specificity of cut off points on receiver operating curve forsemantic verbal fluency.
Additional file 1 of Tuberculosis infection screening in children with close contact: a hospital-based study
Creators: name: Sun, Lin
name: Qi, Xue
name: Guo, Yajie
name: Qi, Hui
name: Li, Jieqiong
name: Wu, Xirong
name: Yin, Qingqin
name: Guo, Yan
name: Xu, Baoping
name: Wang, Yacui
name: Jin, Qi
name: Gao, Lei
name: Shen, Adong
Publisher: name: figshare
Year: 2021
Subjects: Medicine
Biotechnology
Biological Sciences not elsewhere classified
Science Policy
Computational Biology

description: Additional file 1. Additional tables.
title: 03_0914
Creators: name: Wesley Baldwin, John
name: Baldwin, John W.
Publisher: name: NAKALA - https://nakala.fr (Huma-Num - CNRS)
publisherIdentifier: https://ror.org/04ces3204
publisherIdentifierScheme: ROR
Year: 2018
Subjects: subject: LaMOP
subject: Laboratoire de Médiévistique Occidentale de Paris

description: L'ensemble de ces notes ont été crée par John Baldwin lors d'une de ses premières périodes de résidence à Paris. Ces documents comprennent tous les magistri qu’il avait pu rencontrer dans ses sources. Aux XIIème et XIIIème siècles, les universités n'existant pas encore, le titre de magister s'il évoque certainement une compétence n'est pas rattaché de façon systématique au passage par les écoles. De surcroît, les magistris sont le plus souvent désignés par leur seul prénom. La lecture de ces données est donc difficile et suppose un long travail pour tenter d'identifier un individu auquel on pourrait rattacher plusieurs mentions. John Baldwin avait donc renoncé à utiliser ces données pour donner un prolongement à sa magnifique étude sur les gradués d'université et leur emploi par les Capétiens et les Plantagenêts. Néanmoins, il a conservé ces données et elles sont potentiellement très utile dans le cadre de Studium, comme le sont toutes les mentions dans les volumes des Fasti Ecclesiae Gallicanae de chanoines portant ce titre de magister sans autres précisions. L'ampleur des dépouillements rend ces données très utiles et nous devons une grande reconnaissance à John Baldwin pour avoir pris la peine de faire photocopier ses fiches et de nous les avoir transmises amicalement.
Les données ainsi que les images des fiches sont accessibles sur le site Internet à l'adresse suivante : http://studium-baldwin.lamop.fr/.
Les transcriptions des fiches ont été effectuées par des étudiants de Paris 1 Panthéon - Sorbonne mais elles n'ont pas été corrigées par la suite.
description: Relation: https://lamop.hypotheses.org/
FORMATIVE EVALUATION OF AUTHENTIC READING ASSESSMENT MODEL ON BLENDED LEARNING TO PROMOTE SELF-DIRECTED LEARNING SKILLS
Creators: name: Yulmiati
name: M. Zaim
name: Atmazaki
Publisher: name: Zenodo
Year: 2024

description: Abstract

This study aims to investigate the quality of the authentic reading assessment model on blended learning in promoting self-directed learning skills as the research product in the form of a basic reading book. This study follows formative evaluation activities proposed by Tessmer in Plomp & Nieveen (2013) comprising self-evaluation, expert review, one-to-one evaluation, small group, and field test. The selecting participants involve six experts to review the research products in the form of basic reading book, five users in one-to-one evaluation, 15 students in small group, and 35 students in field test. Data were collected through the specification checklist to determine the specification of the research product. Then, a validation sheet was used to get information about the validity, the interview for one-to-one evaluation, and the questionnaires for students and lecturers to determine the practicality. Data of the effectiveness was collected through a comparison of pretest and posttest values. The data were analyzed quantitatively and qualitatively depending on the instruments used. It was found that the components of reading, authentic assessment, blended learning, and self-directed learning achieved the criteria. Viewing the validity of the research product, the results of the experts review show that it was very valid (4.01). Meanwhile, from the result of the one-to-one evaluation, the book was valid from the users’ review. The book was categorized as very practical with a score of 4.22. Authentic reading assessment model on blended learning developed that promotes students’ self-directed learning significantly reached the score of t-calculated 14.17, higher than t-table 2.02. It indicates that the research products were effective in enhancing students’ reading skills. The authentic reading assessment model on blended learning developed achieved quality with high resistance to revision.
Additional file 1 of Systematic review and meta-analysis on the use of human platelet lysate for mesenchymal stem cell cultures: comparison with fetal bovine serum and considerations on the production protocol
Creators: name: Palombella, Silvia
name: Perucca Orfei, Carlotta
name: Castellini, Greta
name: Gianola, Silvia
name: Lopa, Silvia
name: Mastrogiacomo, Maddalena
name: Moretti, Matteo
name: de Girolamo, Laura
Publisher: name: figshare
Year: 2022
Subjects: subject: Space Science
subject: Cell Biology
subject: Molecular Biology
subject: Physiology
subject: FOS: Biological sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Cancer
subject: Science Policy
subject: Hematology

description: Additional file 1. Forest plots of cumulative population doubling for FBS versus HPL at different cell passage for both BMSCs and ASCs.
Additional file 2 of Systematic review and meta-analysis on the use of human platelet lysate for mesenchymal stem cell cultures: comparison with fetal bovine serum and considerations on the production protocol
Creators: name: Palombella, Silvia
name: Perucca Orfei, Carlotta
name: Castellini, Greta
name: Gianola, Silvia
name: Lopa, Silvia
name: Mastrogiacomo, Maddalena
name: Moretti, Matteo
name: de Girolamo, Laura
Publisher: name: figshare
Year: 2022
Subjects: subject: Space Science
subject: Cell Biology
subject: Molecular Biology
subject: Physiology
subject: FOS: Biological sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Cancer
subject: Science Policy
subject: Hematology

description: Additional file 2. Expression of surface markers evaluated by FACS.
Additional file 8 of Assessment of bidirectional relationships between 98 genera of the human gut microbiota and amyotrophic lateral sclerosis: a 2-sample Mendelian randomization study
Creators: name: Zhang, Linjing
name: Zhuang, Zhenhuang
name: Zhang, Gan
name: Huang, Tao
name: Fan, Dongsheng
Publisher: name: figshare
Year: 2022
Subjects: subject: Microbiology
subject: FOS: Biological sciences
subject: Genetics
subject: Ecology
subject: Biological Sciences not elsewhere classified

description: Additional file 8: eFigure 3. Association of genetically predicted amyotrophic lateral sclerosis with OTU4607 Sutterella. Squares represent the effect estimates of the relative abundance ofOTU4607 Sutterellaper 1-unit higher log odds of amyotrophic lateral sclerosis; horizontal lines represent 95% confidence intervals (CIs); diamond represent the effect size with its 95% CI.
Additional file 3 of Systematic review and meta-analysis on the use of human platelet lysate for mesenchymal stem cell cultures: comparison with fetal bovine serum and considerations on the production protocol
Creators: name: Palombella, Silvia
name: Perucca Orfei, Carlotta
name: Castellini, Greta
name: Gianola, Silvia
name: Lopa, Silvia
name: Mastrogiacomo, Maddalena
name: Moretti, Matteo
name: de Girolamo, Laura
Publisher: name: figshare
Year: 2022
Subjects: subject: Space Science
subject: Cell Biology
subject: Molecular Biology
subject: Physiology
subject: FOS: Biological sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Cancer
subject: Science Policy
subject: Hematology

description: Additional file 3. Risk of bias across the studies.
Additional file 4 of Systematic review and meta-analysis on the use of human platelet lysate for mesenchymal stem cell cultures: comparison with fetal bovine serum and considerations on the production protocol
Creators: name: Palombella, Silvia
name: Perucca Orfei, Carlotta
name: Castellini, Greta
name: Gianola, Silvia
name: Lopa, Silvia
name: Mastrogiacomo, Maddalena
name: Moretti, Matteo
name: de Girolamo, Laura
Publisher: name: figshare
Year: 2022
Subjects: subject: Space Science
subject: Cell Biology
subject: Molecular Biology
subject: Physiology
subject: FOS: Biological sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Cancer
subject: Science Policy
subject: Hematology

description: Additional file 4. Sensitivity Analysis—Forest plots of doubling time for FBS 10% versus HPL 10% and FBS10% versus HPL 5%.
Additional file 1 of Liver transplantation in a child with liver cirrhosis caused by langerhans cell histiocytosis: a case report
Creators: name: Wang, Qi
name: Jin, Shuguang
name: Xiang, Bo
name: Chen, Jing
Publisher: name: figshare
Year: 2022
Subjects: Space Science
Physical Sciences not elsewhere classified
Medicine
Biotechnology
Chemical Sciences not elsewhere classified
Cancer
Virology

description: Additional file 1: Figure S1. Hematoxylin and eosin staining of liver biopsy.① the damage and regeneration of liver cells and pseudolobule formation. ② dilation of portal area, proliferation of fibrous tissue and small bile duct. ③ infiltration of inflammation cells. Figure S2. The changes of liver function in this LCH patient. The patient was found to have an abnormal liver function at 20 months old, and was referred to our hospital at 25 months old. Then the living-donor liver transplantation was perfomed when he was 27 months old. ALT: alanine aminotransferase; AST: aspartate aminotransferase; PT: prothrombin time; TB: total bilirubin; ALB: albumin. Figure S3. Macroscopic appearance of the recipient’s liver. a The diaphragmatic surface of liver. b The visceral surface of liver. Figure S4. The enhanced CT of the chest and ultrasound examination of the liver. a-b: the enhanced CT of the chest was performed when the patient was 39 months old and the results were normal; c-d: the ultrasound examination of the liver was performed regularly every 3-6 month since the liver transplantation, and the results were all shown that the echo of the transplanted liver parenchyma was uniform and no dilation of the intrahepatic biliary tract was observed, the presented images were performed when the patient was 45 months old.
FREYA Guest Webinar: CoronaWhy Knowlegde Graph
Creators: name: Tykhonov, Vyacheslav
name: Braukmann, Ricarda
Publisher: name: Zenodo
Year: 2020
Subjects: Persistent Identifiers
Knowlegde Graph
COVID-19
CoronaWhy
CORD-19

description: This upload consists of the presentations and the video recording from the FREYA guest webinar on the CoronaWhy Knowlegde Graph. The webinar was held on Tuesday 1st of September 2020 at 16:00 CEST
Additional file 1 of Potential effect of amniotic fluid-derived stem cells on hyperoxia-induced pulmonary alveolar injury
Creators: name: Solaiman, Amany
name: Mehanna, Radwa A.
name: Meheissen, Ghada A.
name: Elatrebi, Soha
name: Said, Rasha
name: Elsokkary, Nahed H.
Publisher: name: figshare
Year: 2022
Subjects: subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Molecular Biology
subject: Physiology
subject: FOS: Biological sciences
subject: Biotechnology
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Cancer
subject: Computational Biology

description: Additional file 1. Supplementary information file (S1) for figure 7: Full length original western blot analysis for IL-6 and RhoA in all studied experimental groups. Beta actin was used for normalization.
Additional file 1 of Predictors of response to intra-arterial vasodilatory therapy of non-occlusive mesenteric ischemia in patients with severe shock: results from a prospective observational study
Creators: name: Rittgerodt, Nina
name: Pape, Thorben
name: Busch, Markus
name: Becker, Lena S.
name: Schneider, Andrea
name: Wedemeyer, Heiner
name: Seeliger, Benjamin
name: Schmidt, Julius
name: Hunkemöller, Anna Maria
name: Fuge, Jan
name: Knitsch, Wolfgang
name: Fegbeutel, Christine
name: Gillmann, Hans-Jörg
name: Meyer, Bernhard C.
name: Hoeper, Marius M.
name: Hinrichs, Jan B.
name: David, Sascha
name: Stahl, Klaus
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: Science Policy
subject: Hematology
subject: Computational Biology

description: Additional file 1: Figure 1. Standardized diagnostic workup for patients with suspected NOMI. If patients met inclusion criteria, a standardized diagnostic workup following an in house protocol was initiated employing initial biphasic contrast enhanced computed tomography angiography (CT) and digital subtraction angiography (DSA). If both examinations suggested presence of NOMI and excluded complications that required emergency surgical exploration a joint decision (Surgery, Critical Care, Interventional Radiology Team) was made to commence on intra-arterial prostaglandin therapy. If patients or their legal representative gave informed consent, patients were included into the study.
Additional file 1 of Development of a new adapted QuinteT Recruitment Intervention (QRI-Two) for rapid application to RCTs underway with enrolment shortfalls—to identify previously hidden barriers and improve recruitment
Creators: name: Donovan, Jenny L.
name: Jepson, Marcus
name: Rooshenas, Leila
name: Paramasivan, Sangeetha
name: Mills, Nicola
name: Elliott, Daisy
name: Wade, Julia
name: Reda, Domenic
name: Blazeby, Jane M.
name: Moghanaki, Drew
name: Hwang, E. Shelley
name: Davies, Louise
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Cell Biology
subject: Biotechnology
subject: Science Policy
subject: Hematology
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Additional file 1: Table S1. Summary of findings from QRIs attempted with ongoing RCTs with enrolment shortfalls 2007-2017 (n-12). Table S2. Summary of findings from QRI-Two undertaken with actively recruiting RCTs with enrolment shortfalls, 2018-2019 (n-14). Table S3. Summary of findings from QRI-Two undertaken with RCTs at the start-up stage, 2018-2019 (n-6).
Ethics Approval
Creators: name: Hickey, Paige Connors
name: Urry, Heather L.
name: Garay, Maria
name: Pagan, Jeremy
name: Raea E. Rasmussen
name: Eddine, Samer Nour
Publisher: name: OSF Registries
Year: 2020

description: In this component, we uploaded our approval notice from the Tufts University Social, Behavioral, & Educational Research Institutional Review Board. They determined that the study is exempt.
Additional file 2 of Predictors of response to intra-arterial vasodilatory therapy of non-occlusive mesenteric ischemia in patients with severe shock: results from a prospective observational study
Creators: name: Rittgerodt, Nina
name: Pape, Thorben
name: Busch, Markus
name: Becker, Lena S.
name: Schneider, Andrea
name: Wedemeyer, Heiner
name: Seeliger, Benjamin
name: Schmidt, Julius
name: Hunkemöller, Anna Maria
name: Fuge, Jan
name: Knitsch, Wolfgang
name: Fegbeutel, Christine
name: Gillmann, Hans-Jörg
name: Meyer, Bernhard C.
name: Hoeper, Marius M.
name: Hinrichs, Jan B.
name: David, Sascha
name: Stahl, Klaus
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: Science Policy
subject: Hematology
subject: Computational Biology

description: Additional file 2: Figure 2. Angiographic parameters. Violine plots showing analysis of semi-quantitative NOMI score (A), peak density (PD-PV) (B) and time to peak (TTP-PV) (C) in the portal vein as well as area under the curve contrast intensity in the aorta (AU-Aorta) (E) in a subset (n = 40 for NOMI score, n = 30 for 2D-perfusion angiography-related parameters) of NOMI patients before (pre) and directly after (post) initial administration of intra-arterial prostaglandin bolus. Median (IQR) NOMI score decreased following initial prostaglandin administration (pre: 5 (4–7) points vs after: 2 (1–3) points, p
Additional file 3 of Predictors of response to intra-arterial vasodilatory therapy of non-occlusive mesenteric ischemia in patients with severe shock: results from a prospective observational study
Creators: name: Rittgerodt, Nina
name: Pape, Thorben
name: Busch, Markus
name: Becker, Lena S.
name: Schneider, Andrea
name: Wedemeyer, Heiner
name: Seeliger, Benjamin
name: Schmidt, Julius
name: Hunkemöller, Anna Maria
name: Fuge, Jan
name: Knitsch, Wolfgang
name: Fegbeutel, Christine
name: Gillmann, Hans-Jörg
name: Meyer, Bernhard C.
name: Hoeper, Marius M.
name: Hinrichs, Jan B.
name: David, Sascha
name: Stahl, Klaus
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: Science Policy
subject: Hematology
subject: Computational Biology

description: Additional file 3: Figure 3. Biomarkers of intestinal ischemia. Violine plots showing analysis of L-FABP (A), i-FABP (B) and SM22 (C) in NOMI patients (n = 22) at inclusion compared to healthy controls (Ctrl) (n = 20). Median L-FABP concentrations were more than 10 times higher in NOMI patients compared to healthy controls (197 (179–206) ng/ml vs. 16 (10–18) ng/ml, p
Additional file 4 of Predictors of response to intra-arterial vasodilatory therapy of non-occlusive mesenteric ischemia in patients with severe shock: results from a prospective observational study
Creators: name: Rittgerodt, Nina
name: Pape, Thorben
name: Busch, Markus
name: Becker, Lena S.
name: Schneider, Andrea
name: Wedemeyer, Heiner
name: Seeliger, Benjamin
name: Schmidt, Julius
name: Hunkemöller, Anna Maria
name: Fuge, Jan
name: Knitsch, Wolfgang
name: Fegbeutel, Christine
name: Gillmann, Hans-Jörg
name: Meyer, Bernhard C.
name: Hoeper, Marius M.
name: Hinrichs, Jan B.
name: David, Sascha
name: Stahl, Klaus
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: Science Policy
subject: Hematology
subject: Computational Biology

description: Additional file 4: Table 1. Demographic, clinical, angiographic and biochemical characteristics for non-surviving (n = 30) and surviving (n = 12) patients. 28-day mortality was the key secondary outcome. Values are presented as median (25% to 75% interquartile range) or if categorical as numbers and percentages. Peak density (PD), time to peak (TTP) and area under the curve (AUC) are in relation to the superior mesenteric artery (SMA) as reference.
Additional file 1 of Nonoperative treatment for pain sensitization in patients with low back pain: protocol for a systematic review
Creators: name: Nopsopon, Tanawin
name: Suputtitada, Areerat
name: Lertparinyaphorn, Irin
name: Pongpirul, Krit
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Biotechnology
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: Cancer
subject: Science Policy
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Additional file 1. PRISMA-P 2015 Checklist.
title: Bacterial chemoreceptor signaling complexes control kinase activity by stabilizing the catalytic domain of CheA
Creators: name: Tran, Thomas
name: Karunanayake Mudiyanselage, Aruni PKK
name: Eyles, Stephen J.
name: Thompson, Lynmarie K
Publisher: name: University of Massachusetts Amherst
Year: 2023

description: Motile bacteria have a chemotaxis system that enables them to sense their environment and direct their swimming toward favorable conditions. Chemotaxis involves a signaling process in which ligand binding to the extracellular domain of the chemoreceptor alters the activity of the histidine kinase, CheA, bound ~300 Å away to the distal cytoplasmic tip of the receptor, to initiate a phosphorylation cascade that controls flagellar rotation. The cytoplasmic domain of the receptor is thought to propagate this signal via changes in dynamics and/or stability, but it is unclear how these changes modulate the kinase activity of CheA. To address this question, we have used hydrogen deuterium exchange mass spectrometry to probe the structure and dynamics of CheA within functional signaling complexes of the Escherichia coli aspartate receptor cytoplasmic fragment, CheA, and CheW. Our results reveal that stabilization of the P4 catalytic domain of CheA correlates with kinase activation. Furthermore, differences in activation of the kinase that occur during sensory adaptation depend on receptor destabilization of the P3 dimerization domain of CheA. Finally, hydrogen exchange properties of the P1 domain that bears the phosphorylated histidine identify the dimer interface of P1/P1’ in the CheA dimer and support an ordered sequential binding mechanism of catalysis, in which dimeric P1/P1’ has productive interactions with P4 only upon nucleotide binding. Thus stabilization/destabilization of domains is a key element of the mechanism of modulating CheA kinase activity in chemotaxis, and may play a role in the control of other kinases.