title: Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð¼ÐµÑÐ¾Ð´Ð° Ð¿Ð¾Ð´Ð³Ð¾ÑÐ¾Ð²ÐºÐ¸ Ð¾Ð±ÑÐ°Ð·ÑÐ° Ð´Ð»Ñ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ ÑÐºÑÐ¿ÑÐµÑÑ-Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐ¸ Ð²Ð¸ÑÑÑÐ½ÑÑ Ð¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÑ Ð¿Ð°ÑÐ¾Ð³ÐµÐ½Ð¾Ð²
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Development of a sample preparation method for rapid diagnosis of viral and bacterial pathogens
Creators: name: ÐÐ¸ÑÐ°Ð»Ð¾, ÐÐ°ÑÐ°Ð»ÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: CRISPR-CAS
ÑÐºÑÐ¿ÑÐµÑÑ-Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐ°
ÑÐºÑÑÑÐ°ÐºÑÐ¸Ñ
Ð°Ð¼Ð¿Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ñ
expressÂ diagnostics
extraction
amplification

description: ÐÐ°Ð´Ð°ÑÐ¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: - Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐ°ÑÑ ÑÐ¾ÑÑÐ°Ð² Ð»Ð¸Ð·Ð¸ÑÑÑÑÐµÐ³Ð¾ Ð±ÑÑÐµÑÐ°, Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÑÑÐµÐ³Ð¾ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ ÑÐºÑÑÑÐ°Ð³Ð¸ÑÐ¾Ð²Ð°ÑÑ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð» Ð¸Ð· Ð¾Ð±ÑÐ°Ð·ÑÐ°; - Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐ°ÑÑ Ð¿ÑÐ¾ÑÐ¾ÐºÐ¾Ð» ÑÐµÑÐ¼Ð¸ÑÐµÑÐºÐ¾Ð¹ ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ð° Ð¸Ð· Ð¾Ð±ÑÐ°Ð·ÑÐ°; - ÐÑÐµÐ½Ð¸ÑÑ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ ÑÐ¿Ð¾ÑÐ¾Ð±Ð¾Ð² ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ð° Ð¸ Ð²ÑÐ±ÑÐ°ÑÑ Ð¾Ð¿ÑÐ¸Ð¼Ð°Ð»ÑÐ½ÑÐ¹. ÐÐ±ÑÐµÐºÑÐ¾Ð¼ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÑÑÑÑ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÐµ Ð¼ÐµÑÐ¾Ð´Ñ ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ð° Ð¸Ð· Ð¾Ð±ÑÐ°Ð·ÑÐ°. ÐÑÐµÐ´Ð¼ÐµÑÐ¾Ð¼ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐ²Ð»ÑÐµÑÑÑ Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ Ð¿ÑÐ¾ÑÑÐ¾Ð³Ð¾ Ð¸ ÑÐ¾ÑÐ¾ÑÐ¾ Ð¿Ð¾Ð´ÑÐ¾Ð´ÑÑÐµÐ³Ð¾ Ð´Ð»Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ñ Ð² ÑÐµÐ°ÐºÑÐ¸Ð¸ Ð¸Ð·Ð¾ÑÐµÑÐ¼Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð°Ð¼Ð¿Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ ÑÐ¿Ð¾ÑÐ¾Ð±Ð° ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ð° Ð¸Ð· Ð¾Ð±ÑÐ°Ð·ÑÐ°. ÐÑÐ¸ Ð½Ð°Ð¿Ð¸ÑÐ°Ð½Ð¸Ð¸ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±Ð·Ð¾ÑÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ: eLIBRARY.RU, PubMed. Ð¡ÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÑÑ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»Ð¸ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿Ð°ÐºÐµÑÐ° Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼ Microsoft Excel. Ð Ð°Ð±Ð¾ÑÐ° Ð±ÑÐ»Ð° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ ÐÐÐ Â«ÐÐ°Ð½Ð¾Ð±Ð¸Ð¾ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¸Â» Ð¡Ð°Ð½ÐºÑ ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¾Ð³Ð¾ Ð³Ð¾ÑÑÐ´Ð°ÑÑÑÐ²ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑÐ° ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾. Ð ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÑÐ°Ð»Ð¾ ÑÑÐ½Ð¾, ÑÑÐ¾ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ ÑÑÑÐµÐºÑÐ¸Ð²Ð½ÑÐ¼ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ð° Ð¸Ð· Ð¾Ð±ÑÐ°Ð·ÑÐ° ÑÐ²Ð»ÑÐµÑÑÑ Ð¼ÐµÑÐ¾Ð´ Ñ Ð²Ð¾Ð´Ð¾Ð¹ Milli-Q. ÐÑÐµ Ð¿ÑÐ¾ÑÐµÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐµ ÑÐ¾ÑÑÐ°Ð²Ñ Ð±ÑÑÐµÑÐ¾Ð² Ð¿Ð¾ÐºÐ°Ð·Ð°Ð»Ð¸ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ ÑÑÐ¶Ðµ, ÑÐµÐ¼ Ð¼ÐµÑÐ¾Ð´ ÑÐµÑÐ¼Ð¸ÑÐµÑÐºÐ¾Ð¹ ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸ Ð²Ð¾Ð´Ð¾Ð¹ Milli-Q. ÐÑÐ» ÑÐ¾ÑÑÐ°Ð²Ð»ÐµÐ½ Ð¿ÑÐ¾ÑÐ¾ÐºÐ¾Ð» ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸.Â 
description: The tasks of the study: - To develop the composition of a lysing buffer that allows efficient extraction of genetic material from a sample; - To develop a protocol for thermal extraction of genetic material from a sample; - Evaluate the effectiveness of various methods of extracting genetic material and choose the best one. The object of the study of this work is various methods of extraction of genetic material from a sample. The subject of the study is the study of the simplest and most suitable method for extraction of genetic material from a sample for use in an isothermal amplification reaction. When writing the literary review, the following databases were used: eLibrary.RU, PubMed. Statistical processing of experimental data was carried out using the Microsoft Excel software package. The work was carried out in the analytical center of nano- and biotechnologies of Peter the Great St. Petersburg Polytechnic University. During the study, it became clear that the most effective method of extracting genetic material from a sample is the Milli-Q water extraction method. All tested buffer formulations showed worse results than the Milli-Q water extraction method. An extraction protocol was drawn up.