Additional file 1 of A model study for the manufacture and validation of clinical-grade deciduous dental pulp stem cells for chronic liver fibrosis treatment
Creators: name: Iwanaka, Tsuyoshi
name: Yamaza, Takayoshi
name: Sonoda, Soichiro
name: Yoshimaru, Koichiro
name: Matsuura, Toshiharu
name: Haruyoshi Yamaza
name: Ohga, Shouichi
name: Yoshinao Oda
name: Taguchi, Tomoaki
Publisher: name: figshare
Year: 2020
Subjects: subject: Space Science
subject: Medicine
subject: Cell Biology
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: FOS: Chemical sciences
subject: Ecology
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics
subject: FOS: Mathematics
subject: Developmental Biology
subject: Cancer
subject: Hematology

description: Supplementary Methods. Table S1. Summary of population doubling level in human deciduous pulp stem cell (hDPSC) products. Table S2. Summary of expression of cell surface antigens for mesenchymal stem cells in human deciduous pulp stem cell products. Table S3. Summary of expression of cell surface antigens for immunogenicity in human deciduous pulp stem cell products. Table S4. Antibody information for flow cytometry. Table S5. TaqMan probe information for qRT-PCR assay to human adipocyte-, chondrocyte-, and osteoblast-specific genes. Table S6. TaqMan probe information for qRT-PCR assay to human hepatocyte-specific genes. Table S7. TaqMan probe information for real-time RT-PCR. Table S8. Commercially available kit information for biochemical analysis and enzyme-labeled immunosorbent assay. Table S9. Antibody information for immunohistochemistry. Fig. S1. Cell surface marker analysis of the P3 hDPSCs was tested by flow cytometric assay. The results are shown as representative histograms. The red- and black-colored histograms indicate the frequency stained with target antigen-specific and isotype-matched antibodies, respectively. R-PE, Rphycoerythrin. Fig. S2. Cell surface marker analysis of the P10 hDPSCs was tested by flow cytometric assay. The results are shown as representative histograms. The red- and black-colored histograms indicate the frequency stained with target antigen-specific and isotype-matched antibodies, respectively. R-PE, Rphycoerythrin. Fig. S3. Cell surface marker analysis of the P10 hDPSCs was tested by flow cytometric assay. The results are shown as representative histograms. The red- and black-colored histograms indicate the frequency stained with target antigen-specific and isotype-matched antibodies, respectively. R-PE, Rphycoerythrin. Fig. S4. Transplantation of P3 hDPSC products improved chronically CCl4-damaged liver dysfunction in mice. Fig. S5. Anti-fibrotic effects of P10 MCB-hDPSC transplantation in mouse CCl4-induced fibrotic liver. Fig. S6. Cell surface marker analysis of the P10 hDPSCs was tested by flow cytometric assay. Markers for mesenchymal stem cells (a) and immunogenic antigen (b) were assessed in the final hDPSC products. The results are shown as representative histograms. The red- and black-colored histograms indicate the frequency stained with target antigen-specific and isotype-matched antibodies, respectively. R-PE, R-phycoerythrin. Fig. S7. Transplantation of final hDPSC-products from WCB improves CCl4-induced pro-fibrotic markers in mice. (PDF 5848 kb)