Additional file 1 of OsPP65 Negatively Regulates Osmotic and Salt Stress Responses Through Regulating Phytohormone and Raffinose Family Oligosaccharide Metabolic Pathways in Rice
Creators: name: Liu, Qing
name: Ding, Jierong
name: Huang, Wenjie
name: Yu, Hang
name: Wu, Shaowen
name: Li, Wenyan
name: Mao, Xingxue
name: Chen, Wenfeng
name: Xing, Junlian
name: Li, Chen
name: Yan, Shijuan
Publisher: name: figshare
Year: 2022
Subjects: subject: Plant Biology
subject: FOS: Biological sciences

description: Additional file 1: Fig. S1. Phenotypes of the wild-type (WT) and OsPP65 knockout plants at 30 days after flowering under normal conditions. Fig. S2. OsPP65 knockout rice plants show enhanced salt stress tolerance at the germination stage. A, Seeds of the WT and OsPP65 knockout plants were germinated on 1/2 MS medium with (150 and 200 mM) or without (0 mM) NaCl for 7 days. B, Shoot lengths of the germinated seeds of WT and OsPP65 knockout plants. C, Root lengths of the germinated seeds of WT and OsPP65 knockout plants. Data represent means ± SD of three biological replicates (20 plants for each replicate), and the asterisks indicate significant differences compared with the WT plants at *P &lt; 0.05 (Dunnett's test). Fig. S3. The OsPP65 knockout seeds were less sensitive to exogenous ABA relative to the wild-type seeds. A, Photograph showing the germinated seeds of WT and OsPP65 knockout plants with or without ABA treatment. B, Shoot lengths of the germinated seeds of WT and OsPP65 knockout plants. C, Root lengths of the germinated seeds of WT and OsPP65 knockout plants. Data represent means ± SD of three biological replicates (20 plants for each replicate) and the asterisks indicate significant differences compared to the WT plants at *P &lt; 0.05 (Dunnett's test). Fig. S4. The OsPP65 knockout seeds were less sensitive to exogenous JA relative to the WT seeds. A, Phenotypes of the germinated seeds of WT and OsPP65 knockout plants with or without JA treatment. B, Shoot lengths of the germinated seeds of WT and OsPP65 knockout plants. C, Root lengths of the germinated seeds of WT and OsPP65 knockout plants. Data represent means ± SD of three biological replicates (20 plants for each replicate) and the asterisks indicate significant differences compared to the WT plants at *P &lt; 0.05 (Dunnett's test). Fig. S5. Exogenous ABA treatment did not affect the JA signaling pathway in OsPP65 knockout plants. A, Transcription analysis of four JA biosynthesis genes in the WT and OsPP65 knockout plants before and after ABA treatment. B, Quantification of endogenous JA in the WT and OsPP65 knockout plants before and after ABA treatment. FW, fresh weight. Fig. S6. Exogenous JA treatment did not affect the JA signaling pathway in OsPP65 knockout plants. A, Transcription analysis of two ABA biosynthesis genes and five OsLEA genes in the WT and OsPP65 knockout plants before and after JA treatment. Data represent means ± SD of three biological replicates and the asterisks indicate significant differences compared to the WT plants at **P &lt; 0.01 and *P &lt; 0.05 (Dunnett's test). B, Quantification of endogenous JA in the WT and OsPP65 knockout plants before and after JA treatment. FW, fresh weight. Fig. S7. Score plot generated from the GC–MS metabonomics data using the crossvalidated OPLS-DA model. Fig. S8. The fold changes of other sugars or amino acids in WT and OsPP65 knockout plants at 4 h osmotic stress treatment relative to 0 h treatment. Data represent means ± SD of three biological replicates and the asterisks indicate significant differences compared to the WT plants at **P &lt; 0.01 and *P &lt; 0.05 (Dunnett's test). Fig. S9. Expression levels of OsSPCH2 and OsICE1 in WT and OsPP65 knockout plants under normal condition. Data represent means ± SD of three biological replicates and the asterisks indicate significant differences compared to the WT plants at *P &lt; 0.05 and **P &lt; 0.01 (Dunnett's test). Fig. S10. Expression levels of OsJAR1 in WT and OsPP65 knockout plants with or without osmotic stress treatment. Data represent means ± SD of three biological replicates and the asterisks indicate significant differences compared to the WT plants at *P &lt; 0.05 (Dunnett's test).