Comparison Between The Effects Of Caspafungin, Amphotericin B And Ketocanazole On Cytokine Levels Of Thp-1 Cells Activated By Lps
Creators: name: Canturk, Zerrin
Publisher: name: Zenodo
Year: 2016
Subjects: THP-1
cytokine
caspafungin

description: Cytokines are polypeptide molecules that regulate immune and inflammatory reactions by secreting antimicrobial or other antigens. To investigate the role of cytokines in inflammation, THP-1 monocyte cell line is generally used as a model in literature. In immune regulation and immune response determination studies, interleukin 1?, interleukin 6, interleukin 8 and tumor necrosis factor-? that play role in proinflammation and interleukin 10 that play role in antiinflammation are the most important and commonly used cytokines. IkB complex phosphorylated by lipopolysaccharides (LPS) that produced by gram negative bacteria stimulation and NFkB that translocated into the cell nucleus in different genes activate the mediators that responsible from proinflammatory response. In this study, amphotericin b, caspofungin and ketaconazole were administered before and after LPS treatment on THP-1 cells. Cytokine levels of the supernatants that collected after treatment, were evaluated by Cytometric Bead Array. Tumor necrosis factor and interleukin 8 are the preliminary cytokines that secrete by monocytes in immune response to microbial products. As a result, the highest interleukin 8 levels were obtained by amphotericin b (195.59 pg/mL) treatment before LPS and the proinflammatory effect were continued. Caspofungin (657.11 pg/mL) and ketaconazole (254.44 pg/mL) were found effective as antiinflammatory. The results described in our study clearly indicate that the microbicidal activity of human monocytes is closely associated with the activities of proinflamatory cytokines.Cytokines are polypeptide molecules that regulate immune and inflammatory reactions by secreting antimicrobial or other antigens. To investigate the role of cytokines in inflammation, THP-1 monocyte cell line is generally used as a model in literature. In immune regulation and immune response determination studies, interleukin 1?, interleukin 6, interleukin 8 and tumor necrosis factor-? that play role in proinflammation and interleukin 10 that play role in antiinflammation are the most important and commonly used cytokines. IkB complex phosphorylated by lipopolysaccharides (LPS) that produced by gram negative bacteria stimulation and NFkB that translocated into the cell nucleus in different genes activate the mediators that responsible from proinflammatory response. In this study, amphotericin b, caspofungin and ketaconazole were administered before and after LPS treatment on THP-1 cells. Cytokine levels of the supernatants that collected after treatment, were evaluated by Cytometric Bead Array. Tumor necrosis factor and interleukin 8 are the preliminary cytokines that secrete by monocytes in immune response to microbial products. As a result, the highest interleukin 8 levels were obtained by amphotericin b (195.59 pg/mL) treatment before LPS and the proinflammatory effect were continued. Caspofungin (657.11 pg/mL) and ketaconazole (254.44 pg/mL) were found effective as antiinflammatory. The results described in our study clearly indicate that the microbicidal activity of human monocytes is closely associated with the activities of proinflamatory cytokines.