title: ÐÐ·Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ Ð¾ÑÐ½Ð¾ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ ÑÐµÑÐ¼ÐµÐ½ÑÐ¾Ð² ÑÐ¸ÑÑÐµÐ¼Ñ ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ II ÑÐ¸Ð¿Ð° Ð¿ÑÐ¸ Ð·Ð°ÑÐ°Ð¶ÐµÐ½Ð¸Ð¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð¾Ð¼
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Change in the relative concentration of enzymes of the restriction-modification system of type II upon infection of bacterial cells with a bacteriophage
Creators: name: ÐÐ½Ð¾Ð±Ð¸ÑÐµÐ²Ð°, ÐÐ»ÐµÐ½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: ÑÐ¸ÑÑÐµÐ¼Ð° ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸
esp396i
Ð¼ÐµÑÐ¸Ð»ÑÑÐ°Ð½ÑÑÐµÑÐ°Ð·Ð°
ÑÐ½Ð´Ð¾Ð½ÑÐºÐ»ÐµÐ°Ð·Ð° ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸
Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³ Î»
restriction-modification system
methyltransferase
restriction endonuclease
bacteriophage Î»

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«ÐÐ·Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ Ð¾ÑÐ½Ð¾ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ ÑÐµÑÐ¼ÐµÐ½ÑÐ¾Ð² ÑÐ¸ÑÑÐµÐ¼Ñ ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ II ÑÐ¸Ð¿Ð° Ð¿ÑÐ¸ Ð·Ð°ÑÐ°Ð¶ÐµÐ½Ð¸Ð¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð¾Ð¼Â». ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð¾ÑÐ½Ð¾ÑÐ¸ÑÐµÐ»ÑÐ½ÑÑ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¹ Ð±ÐµÐ»ÐºÐ¾Ð² ÑÐ¸ÑÑÐµÐ¼Ñ ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ (Ð -Ð) Esp1396I Ð² Ð¾ÑÐ´ÐµÐ»ÑÐ½ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ Escherichia coli Ð½Ð° ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð¿ÑÐµÐ¾Ð´Ð¾Ð»ÐµÐ½Ð¸Ñ Ð¸Ñ Ð·Ð°ÑÐ¸ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð¾Ð¼ Î»vir Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¿ÑÐ¾ÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐ¸ÑÐ¾Ð¼ÐµÑÑÐ¸Ð¸. ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð¿Ð¾ ÑÐ¾Ð´Ñ ÑÐ°Ð±Ð¾ÑÑ: 1) ÐÐ·Ð¼ÐµÑÐµÐ½Ð¸Ðµ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ Escherichia coli, Ð½ÐµÑÑÑÐ¸Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾-Ð¼ÐµÑÐµÐ½Ð½ÑÑ ÑÐ¸ÑÑÐµÐ¼Ñ Ð -Ð Esp1396I Ð² ÐºÐ°Ð½Ð°Ð»Ð°Ñ Venus (ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²ÑÐµÑ Ð¼ÐµÑÐ¸Ð»ÑÑÐ°Ð½ÑÑÐµÑÐ°Ð·Ðµ, Ð) Ð¸ mCherry (ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²ÑÐµÑ ÑÐ½Ð´Ð¾Ð½ÑÐºÐ»ÐµÐ°Ð·Ðµ ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸, Ð­Ð ), Ð¿ÑÐ¸ Ð·Ð°ÑÐ°Ð¶ÐµÐ½Ð¸Ð¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð¾Ð¼ Î»vir Ð¸ Ð±ÐµÐ· Ð·Ð°ÑÐ°Ð¶ÐµÐ½Ð¸Ñ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¿ÑÐ¾ÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐ¸ÑÐ¾Ð¼ÐµÑÑÐ¸Ð¸. 2) ÐÐ·Ð¼ÐµÑÐµÐ½Ð¸Ðµ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ Escherichia coli, Ð½ÐµÑÑÑÐ¸Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾-Ð¼ÐµÑÐµÐ½Ð½ÑÑ ÑÐ¸ÑÑÐµÐ¼Ñ Ð -Ð Esp1396I Ñ Ð´Ð¾Ð¿Ð¾Ð»Ð½Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÐºÑÐ¿ÑÐµÑÑÐ¸ÐµÐ¹ Ð Ð¸ Ð­Ð  Ð² ÐºÐ°Ð½Ð°Ð»Ð°Ñ Venus (ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²ÑÐµÑ Ð) Ð¸ mCherry (ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²ÑÐµÑ Ð­Ð ), Ð¿ÑÐ¸ Ð·Ð°ÑÐ°Ð¶ÐµÐ½Ð¸Ð¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð¾Ð¼ Î»vir Ð¸ Ð±ÐµÐ· Ð·Ð°ÑÐ°Ð¶ÐµÐ½Ð¸Ñ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¿ÑÐ¾ÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐ¸ÑÐ¾Ð¼ÐµÑÑÐ¸Ð¸. 3) ÐÐ½Ð°Ð»Ð¸Ð· ÑÐ°ÑÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ð¹ Ð¿Ð¾ Ð¸Ð½ÑÐµÐ½ÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ Escherichia coli, Ð½ÐµÑÑÑÐ¸Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾-Ð¼ÐµÑÐµÐ½Ð½ÑÑ ÑÐ¸ÑÑÐµÐ¼Ñ Ð -Ð Esp1396I Ð¸ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ ÑÑÐ¾Ð³Ð¾ ÑÐ°ÑÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¿ÑÐ¸ Ð·Ð°ÑÐ°Ð¶ÐµÐ½Ð¸Ð¸ ÐºÐ»ÐµÑÐ¾Ðº Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð¾Ð¼ Î»vir. Ð Ð°Ð±Ð¾ÑÐ° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ Ð¡ÐÐ±ÐÐ£ Ð² ÐÐÐ Â«ÐÐ°Ð½Ð¾Ð±Ð¸Ð¾ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¸Â», Ð³Ð´Ðµ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð¾ÑÐ½Ð¾Ð²Ð½Ð°Ñ ÑÐ°ÑÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: ÑÐºÐ¾Ð½ÑÑÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ñ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÐµ ÑÑÐ°Ð¼Ð¼Ñ, Ð½ÐµÑÑÑÐ¸Ðµ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾-Ð¼ÐµÑÐµÐ½ÑÑ ÑÐ¸ÑÑÐµÐ¼Ñ Ð -Ð Esp1396I, Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸ Ð¸ Ð¿ÑÐ¾ÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐ¸ÑÐ¾Ð¼ÐµÑÑÐ¸Ð¸ ÑÐ¾Ð±ÑÐ°Ð½Ñ Ð´Ð°Ð½Ð½ÑÐµ Ð¾ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²ÐµÐ½Ð½Ð¾Ð¼ ÑÐ°ÑÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ð¸ Ð±ÐµÐ»ÐºÐ¾Ð² ÑÐ¸ÑÑÐµÐ¼Ñ Ð -Ð Ð² Ð¾Ð´Ð¸Ð½Ð¾ÑÐ½ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ ÑÐ´Ð°Ð»Ð¾ÑÑ Ð¾Ð¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ, ÐºÐ°ÐºÐ¸Ð¼ Ð¾Ð±ÑÐ°Ð·Ð¾Ð¼ Ð¾ÑÐ½Ð¾ÑÐ¸ÑÐµÐ»ÑÐ½ÑÐµ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ Ð±ÐµÐ»ÐºÐ¾Ð² ÑÐ¸ÑÑÐµÐ¼Ñ Ð -Ð Esp1396I Ð² Ð¾ÑÐ´ÐµÐ»ÑÐ½ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ Escherichia coli Ð²Ð»Ð¸ÑÑÑ Ð½Ð° ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð¿ÑÐµÐ¾Ð´Ð¾Ð»ÐµÐ½Ð¸Ñ Ð·Ð°ÑÐ¸ÑÐ½Ð¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ñ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð¾Ð¼. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ Ð´Ð°Ð½Ð½ÑÐµ Ð¼Ð¾Ð³ÑÑ Ð²Ð½ÐµÑÑÐ¸ Ð±Ð¾Ð»ÑÑÐµÐµ Ð¿Ð¾Ð½Ð¸-Ð¼Ð°Ð½Ð¸Ðµ Ð² Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ ÑÐ¸ÑÑÐµÐ¼ Ð -Ð ÑÐ¸Ð¿Ð° II, Ð°, ÑÐ°ÐºÐ¶Ðµ, Ð½Ð°Ð¹ÑÐ¸ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ Ð² ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐµ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð² Ð¿ÑÐ¾ÑÐ¸Ð² Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÑ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ð¹, Ð¸Ð¼ÐµÑÑÐ¸Ñ ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾ÑÑÑ Ðº Ð°Ð½ÑÐ¸Ð±Ð¸Ð¾ÑÐ¸ÐºÐ°Ð¼.
description: The subject of the graduate qualification work is âChange in the relative concentration of enzymes of the restriction-modification system of type II upon infection of bacterial cells with a bacteriophageâ. This work is devoted to determining the influence of the relative concentrations of the proteins of the restriction-modification system (R-M) Esp1396I in individual bacterial cells of Escherichia coli on the effectiveness of overcoming their protection by the bacteriophage Î»vir using flow cytometry. To achieve the main aim of the study the following objectives have been formulated: 1) Fluorescence measurement of Escherichia coli bacteria carrying the fluorescently labelled R-M system Esp1396I in Venus channels (corresponds to methyltransferase, M) and mCherry (corresponds to restriction endonuclease, R) during infection with bacteriophage Î»vir and without infection by flow cytometry. 2) Fluorescence measurement of Escherichia coli bacteria carrying the fluorescently labelled R-M system Esp1396I with additional expression of M and R in the Venus channels (corresponds to M) and mCherry (corresponds to R) during infection with bacteriophage Î»vir and without infection by flow cytometry. 3) Analysis of the fluorescence intensity distribution of Escherichia coli bacteria carrying the fluorescently labelled R-M system Esp1396I and a change in this distribution upon infection of the cells with bacteriophage Î»vir. The work was carried out on the basis of SPbPU in the Center for âNanobiotechnologyâ, where a significant part of the study was performed: bacterial strains carrying the fluorescence-labelled R-M system Esp1396I were constructed; using fluorescence microscopy and flow cytometry data on the quantitative distribution of proteins of the R-M system in single cells were collected. As a result, we determined how the relative concentrations of proteins of the R-M system Esp1396I affect the efficiency of overcoming the protective system by the bacteriophage in individual bacterial cells of Escherichia coli. The data obtained can make a better understanding of the R-M systems type II mechanism of action, and also find application in the development of drugs against bacterial diseases that are resistant to antibiotics.