cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
title: 01_1570
Creators: name: Wesley Baldwin, John
name: Baldwin, John W.
Publisher: name: NAKALA - https://nakala.fr (Huma-Num - CNRS)
publisherIdentifier: https://ror.org/04ces3204
publisherIdentifierScheme: ROR
Year: 2018
Subjects: subject: LaMOP
subject: Laboratoire de Médiévistique Occidentale de Paris

description: L'ensemble de ces notes ont été crée par John Baldwin lors d'une de ses premières périodes de résidence à Paris. Ces documents comprennent tous les magistri qu’il avait pu rencontrer dans ses sources. Aux XIIème et XIIIème siècles, les universités n'existant pas encore, le titre de magister s'il évoque certainement une compétence n'est pas rattaché de façon systématique au passage par les écoles. De surcroît, les magistris sont le plus souvent désignés par leur seul prénom. La lecture de ces données est donc difficile et suppose un long travail pour tenter d'identifier un individu auquel on pourrait rattacher plusieurs mentions. John Baldwin avait donc renoncé à utiliser ces données pour donner un prolongement à sa magnifique étude sur les gradués d'université et leur emploi par les Capétiens et les Plantagenêts. Néanmoins, il a conservé ces données et elles sont potentiellement très utile dans le cadre de Studium, comme le sont toutes les mentions dans les volumes des Fasti Ecclesiae Gallicanae de chanoines portant ce titre de magister sans autres précisions. L'ampleur des dépouillements rend ces données très utiles et nous devons une grande reconnaissance à John Baldwin pour avoir pris la peine de faire photocopier ses fiches et de nous les avoir transmises amicalement.
Les données ainsi que les images des fiches sont accessibles sur le site Internet à l'adresse suivante : http://studium-baldwin.lamop.fr/.
Les transcriptions des fiches ont été effectuées par des étudiants de Paris 1 Panthéon - Sorbonne mais elles n'ont pas été corrigées par la suite.
description: Relation: https://lamop.hypotheses.org/
Chemical constituents and antileukemic activity of Eugenia dysenterica
Creators: name: Vitek, Renan
name: Leice M. R. De Novais
name: Heron F. V. Torquato
name: Paredes-Gamero, Edgar J.
name: Carvalho, Mário G. De
name: Sousa, Paulo T. De
name: Jacinto, Marcos J.
name: Silva, Virgínia C. Da
Publisher: name: Taylor & Francis
Year: 2016
Subjects: subject: Biochemistry
subject: Genetics
subject: FOS: Biological sciences
subject: Pharmacology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Cancer

description: The study about Eugenia dysenterica led to the isolation of 3-acetyl-urs-12-en-28-oic (1), 3-acetyl-olean-12-en-28-oic acid (2) and isoquercetin (3) from the stem barks, and of 3-O-β-glucopyranosyl-β-sitosterol (4), methyl 3-hydroxy-4-methoxybenzoate (5), methyl 4-hydroxyphenyl propionate (6), E-methyl-4-hydroxycinnamate (7), quercetin-3-O-(6ꞌꞌ-O-galloyl)-β-d-glucopyranoside (8) and quercetin-3-O-β-d-galactopyranoside (9) from the leaves. The structures 1–9 were set through the analysis of their NMR spectroscopic data. Compounds 2, 3 and 5–8 were reported for the first time in the Eugenia genus. Compound 8 reduced cell viability and presented IC50 values 40.3 and 36.7 μM, for the CCRF-CEM and the Kasumi-1 cells, respectively.
Ação da leptina no metabolismo da glucose das células de Sertoli: impacto na fertilidade masculina
Creators: name: Martins, Ana D.
name: Moreira, Ana C.
name: Sá, Rosália
name: Monteiro, Mariana P.
name: Sousa, Mário
name: Carvalho, Rui A.
name: Silva, Branca M.
name: Oliveira, Pedro F.
name: Alves, Marco G.
Publisher: name: Revista Captar: Ciência e Ambiente para Todos
Year: 2016

description: Nos últimos anos os hábitos alimentares e o estilo de vida têm vindo a tornar-se pouco saudáveis, favorecendo o desenvolvimento de doenças metabólicas (por exemplo Diabetes tipo 2 e obesidade). A leptina é uma hormona produzida no tecido adiposo responsável pela sensação de saciedade. Os níveis desta hormona estão positivamente relacionados com a quantidade de gordura corporal e têm sido descritos efeitos negativos associados ao aumento da sua concentração na função reprodutora masculina. [...]
description: Revista Captar: Ciência e Ambiente para Todos, vol. 6 n.º 1 (2016)
title: The Codex – an Atlas of Relations
Creators: name: Neill, Iian
name: Kuczera, Andreas
Publisher: name: Herzog August Bibliothek
Year: 2019

description: Die Modellierung des Zweifels – Schlüsselideen und -konzepte zur graphbasierten Modellierung von Unsicherheiten.
            [Ausgewählte Beiträge der Tagung 19.-20.01.2018 an der Akademie der Wissenschaften und der Literatur, Mainz]
            Hg. von Andreas Kuczera / Thorsten Wübbena / Thomas Kollatz. Wolfenbüttel 2019. (= Zeitschrift für digitale Geisteswissenschaften / Sonderbände, 4)
description: In diesem Beitrag wird The Codex vorgestellt, ein Projekt, in dem basierend auf Standoff Properties Texte multidimensional annotiert und die Annotationen in eine Graphdatenbank eingebettet werden können. Darüberhinaus sind Basistext und Annotationen im Unterschied zu vielen anderen Standoff-Markup-Systemen editierbar. Auch Annotationen selbst können wieder annotiert werden, was den Forschungsdiskurs leichter nachvollziehbar machen könnte.
ENDOGENOUS LEVEL OF ACETIC ACID IN YELLOWFIN TUNA (Thunnus albacares): A PILOT STUDY ABOUT A POSSIBLE CONTROVERSY ON ITS RESIDUE NATURE
Creators: name: Chiesa, Luca Maria
name: Pasquale, Elisa
name: Panseri, Sara
name: Britti, Domenico
name: Malandra, Renato
name: Villa, Roberto
name: Arioli, Francesco
Publisher: name: Taylor & Francis
Year: 2016
Subjects: subject: Biophysics
subject: Molecular Biology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: FOS: Biological sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Inorganic Chemistry
subject: Science Policy

description: A method based on headspace solid-phase microextraction (SPME) followed by GC-MS analysis was developed for the determination of underivatized acetic acid in fresh tuna fish muscle. Parameters such as the fibre selected and the extraction time and temperature were optimised and the linearity, detection limits and precision of the whole analytical procedure were assessed. The method was then applied to determine the acetic acid concentration in fresh Yellowfin tuna muscles (Thunnus albacares), in order to evaluate the endogenous level and its variations during the shelf life under different storage conditions. A qualitative comparison was also made with variations in histamine levels, to evaluate the possibility of the joint monitoring of acetic acid and histamine to identify fish stored in poor conditions. The caudal area always had a lower content of acetic acid than the ventral area, independent of the storage time and temperature. A difference was found between the six-day and three-day time points and day 0 at a storage temperature of 8 °C and between the six-day time point and day 0 at a storage temperature of 0 °C, independent of the anatomical area of the sampled tissue. The evaluation of acetic acid could represent an important approach in the field of food safety, to detect the illicit use of acetic acid as an antibacterial preservative treatment or to eliminate the unpleasant smell of trimethylamine.
Role of Endoplasmic Reticulum stress in sensitization induced dendritic-like cell maturation/toxicity
Creators: name: Luís, Andreia
name: Neves, Bruno
Publisher: name: Revista Captar: Ciência e Ambiente para Todos
Year: 2016

description: The pathogenesis of allergic contact dermatitis, the most common manifestation of immunotoxicity in humans, is intimately connected to hapten-induced maturation of dendritic cells (DC). The molecular mechanisms driving this maturational program are not completely known, however, initial danger signals such as the generation of reactive oxygen species (ROS) were shown to play a critical role. Recent evidence linking ROS production, endoplasmic reticulum (ER) stress and the pathogenesis of several inflammatory diseases led us to analyze, in the present work, the ability of the skin sensitizer 1-fluoro-2,4dinitrobenzene (DNFB) to evoke ER stress in DC-like THP-1 cells and the concomitant consequences to their immunobiology. [...]
description: Revista Captar: Ciência e Ambiente para Todos, vol. 6 n.º 1 (2016)
The speed barrier
Creators: name: Alapat, Daniel
Publisher: name: figshare
Year: 2016
Subjects: subject: Special Relativity
subject: General Relativity
subject: 20105 General Relativity and Gravitational Waves
subject: FOS: Physical sciences

description: The speed of light is one of the most important topics in physics. It is so important because it tells us so much about our universe. The speed of light is the maximum speed at which any particle can travel, it’s the ultimate speed limit. The funny part is that we don’t know why it is so. This speed limits prohibits time travel and prevents us from going back in time like doctor who. In this paper I discuss different cases where the speed limit is thought to broken, why it makes meeting my ‘brother from another plant’ improbable and what happens when we try to break such rules in physics. I also discuss the very interesting theories of relativity and why light is special, it does not accelerate. So as soon as you light a match stick the light moves at the speed of light it doesn’t take time to acquire that speed.
Effects of Di-(2-ethylhexyl) phthalate on vascular smooth muscle cells
Creators: name: Mariana, Melissa
name: Cairrão, Elisa
name: Verde, Ignacio
Publisher: name: Revista Captar: Ciência e Ambiente para Todos
Year: 2016

description: Every year million tons of plastic are produced around the world and humans are increasingly exposed to them. This constant exposure has raised some concerns against human health and substances leaching from plastics, such as phthalates. These compounds, used to give flexibility to polyvinyl chloride (PVC), are endocrine-disrupting compounds because they have the ability to interfere with hormone pathways. Phthalates are not covalently bound to the plastic, which makes them susceptible to go to the atmosphere, foods and consequently into body fluids. [...]
description: Revista Captar: Ciência e Ambiente para Todos, vol. 6 n.º 1 (2016)
Identificação das isoformas Aquaporina 4 e 9 e a sua interação com o CFTR (regulador de condutância trans-membranar de fibrose cística) em células de Sertoli de rato
Creators: name: Jesus, Tito T.
name: Bernardino, Raquel L.
name: Martins, Ana D.
name: Sá, Rosália
name: Sousa, Mário
name: Alves, Marco G.
name: Oliveira, Pedro F.
Publisher: name: Revista Captar: Ciência e Ambiente para Todos
Year: 2016

description: As células de Sertoli (SCs) formam a barreira hematotesticular (BHT), controlando o microambiente onde a espermatogénese ocorre. O regulador de condutância transmembranar de fibrose cística (CFTR) tem um papel preponderante na fertilidade masculina, pois indivíduos com mutações no gene CFTR são normalmente sub-férteis ou inférteis. Para além de ser responsável pelo transporte de Cl-/HCO3-, o CFTR pode controlar o movimento de fluidos, regulando sistemas de transporte de água (Aquaporinas). [...]
description: Revista Captar: Ciência e Ambiente para Todos, vol. 6 n.º 1 (2016)
Séraphin de Flines du Fresnoy
Creators: name: Plouvain, Pierre-Antoine-Samuel-Joseph (1754-1832)
Publisher: name: NAKALA - https://nakala.fr (Huma-Num - CNRS)
publisherIdentifier: https://ror.org/04ces3204
publisherIdentifierScheme: ROR
Year: 2017
Subjects: Officier de justice d'Ancien Régime

description: Source: http://www.sudoc.fr/080675700
description: Coverage: Parlement de Flandre
Confounding in longitudinal studies in addiction treatment research
Creators: name: Pierce, Matthias
name: Dunn, Graham
name: Millar, Tim
Publisher: name: Taylor & Francis
Year: 2016
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Evolutionary Biology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics
subject: Science Policy
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: 60506 Virology

description: Background: The effectiveness of treatment for people with substance use disorders is usually examined using longitudinal cohorts. In these studies, treatment is often considered as a time-varying exposure. The aim of this commentary is to examine confounding in this context, when the confounding variable is time-invariant and when it is time-varying. Method: Types of confounding are described with examples and illustrated using path diagrams. Simulations are used to demonstrate the direction of confounding bias and the extent that it is accounted for using standard regression adjustment techniques. Results: When the confounding variable is time invariant or time varying and not influenced by prior treatment, then standard adjustment techniques are adequate to control for confounding bias, provided that in the latter scenario the time-varying form of the variable is used. When the confounder is time varying and affected by prior treatment status (i.e. it is a mediator of treatment), then standard methods of adjustment result in inconsistency. Conclusions: In longitudinal cohorts where treatment exposure is time varying, confounding is an issue which should be considered, even if treatment exposure is initially randomized. In these studies, standard methods of adjustment may result be inadequate, even when all confounders have been identified. This occurs when the confounder is also a mediator of treatment. This is a likely scenario in many studies in addiction.
Adult demography, spatial distribution and movements of Phengaris alcon (Lepidoptera: Lycaenidae) populations in Portugal
Creators: name: Gonzalez, Darinka
name: Oliveira, Irene
name: Pacheco, Rita
name: Lopes, Domingos
name: Arnaldo, Paula Seixas
Publisher: name: Revista Captar: Ciência e Ambiente para Todos
Year: 2016

description: Herein we present the results of the first mark-release-recapture (MRR) study on five populations of Phengaris alcon near Lamas D´Ôlo, a small village located in the Natural Park of Alvão (PNA) in Portugal. For the study of the adult demographic parameters, a MRR study was conducted during the flight period on the five meadow sites, named: Li: Libania; PM: Plana dos Mijaceiros; PR: Ponte Romana; FM: Frente do Moinho and MO: Moinho near Lamas D´Ôlo Village. The data were analysed using MARK 8.0 software according to Cormack-Jolly-Seber type constrained models to obtain the estimates of survival and capture probability for each population site. [...]
description: Revista Captar: Ciência e Ambiente para Todos, vol. 6 n.º 1 (2016)
Chromium(III), manganese(II) and iron(III) complexes based on hydrazone Schiff-base and azide ligands: synthesis, crystal structure and antimicrobial activity
Creators: name: Shaabani, Behrouz
name: Khandar, Ali Akbar
name: Ramazani, Nahid
name: Fleck, Michel
name: Haedeh Mobaiyen
name: Cunha-Silva, Luís
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Biophysics
subject: Biochemistry
subject: 29999 Physical Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: Cell Biology
subject: Molecular Biology
subject: Pharmacology
subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences

description: A tridentate NNO donor hydrazine Schiff base, HL, was obtained from condensation of pyridine 2-carbaldehyde and 4-hydroxy benzohydrazide. HL and azide ligands with Cr(III), Mn(II) and Fe(III) have been used to synthesize [Cr(L)(N3)(OCH3)]2 (1), [Mn(HL)2(N3)2] (2), and [Fe(L)(N3)(OCH3)]2·H2O (3). HL is quite diverse in its chelating ability and can be a neutral or monoanionic ligand as a tridentate unit. In this paper, we report structures showing different denticities of the ligand having different charges. The ligand 1–3 was characterized by elemental analysis, FT-IR, and UV–vis spectral studies and solid-state structures were determined by single-crystal X-ray diffraction analysis, revealing that 1 and 3 are binuclear, while 2 is mononuclear. The efficiencies of the ligand and the three complexes were evaluated for antimicrobial activity; MIC data revealed that HL 1–3 are not strongly active in comparison to standard drugs.
Neuronal intermediate filament inclusion disease may be incorrectly classified as a subtype of FTLD-FUS
Creators: name: Bieniek, Kevin F
name: Josephs, Keith Anthony
name: Lin, Wen-Lang
name: Dickson, Dennis W
Publisher: name: Free Neuropathology
Year: 2020

description: Background: The majority of cases of frontotemporal lobar degeneration (FTLD) are characterized by focal cortical atrophy with an underlying tau or TDP-43 proteinopathy. A subset of FTLD cases, however, lack tau and TDP-43 immunoreactivity, but have neuronal inclusions positive for ubiquitin, referred to as atypical FTLD (aFTLD-U). Studies have demonstrated that ubiquitin-positive inclusions in aFTLD-U are immunoreactive for fused in sarcoma (FUS). As such, the current nosology for this entity is FTLD-FUS, which is thought to include not only aFTLD-U but also neuronal intermediate filament inclusion disease (NIFID) and basophilic inclusion body disease. Objective: To compare pathological features of cases of aFTLD-U and NIFID. Methods: We reviewed the neuropathology of 15 patients (10 males and 5 females; average age at death 54 years (range 41-69 years)) with an antemortem clinical diagnosis of a frontotemporal dementia and pathological diagnosis of aFTLD-U (n=8) or NIFID (n=7). Sections were processed for immunohistochemistry and immunoelectron microscopy with FUS, TDP-43, and α-internexin (αINX) antibodies. Results: Eight cases had pathologic features consistent with FTLD-FUS, with severe striatal atrophy (7/8 cases), as well as FUS-positive neuronal cytoplasmic and vermiform intranuclear inclusions, but no αINX immunoreactivity. Five cases had features consistent with NIFID, with neuronal inclusions positive for both FUS and αINX. Striatal atrophy was present in only two of the NIFID cases. Two cases had αINX-positive neuronal inclusions consistent with NIFID, but both lacked striatal atrophy and FUS immunoreactivity. Surprisingly, one of these two NIFID cases had lesions immunoreactive for TDP-43. Discussion: While FUS pathology remains a prominent feature of aFTLD-U, there is pathologic heterogeneity, including rare cases of NIFID with TDP-43- rather than FUS-positive inclusions.
description: Free Neuropathology, Bd. 1 (2020)
Diversidade e sensibilidade da microbiota da pele de rãs de um local contaminado e de um local de referência a Co e Cd
Creators: name: Gonçalves, Ana C. D.
name: Figueiredo, Daniela
name: Pereira, Ruth
name: Gonçalves, Fernando
name: Marques, Sérgio M.
Publisher: name: Revista Captar: Ciência e Ambiente para Todos
Year: 2016

description: A microbiota da pele dos anfíbios é considerada parte do seu sistema imunitário já com provas dadas de inclusivamente poder inibir o fungo patogénico Batrachochytrium dendrobatidis. A composição do microbiota pode variar entre locais dependendo de vários parâmetros ambientais. Considerando que alguns contaminantes como os metais são persistentes, podendo afetar as comunidades bacterianas, e que inclusivamente algumas espécies anfíbias como a rã verde (Pelophylax perezi) podem ser encontradas em locais contaminados por metais, é essencial estudar a composição do seu microbiota, bem como a sensibilidade deste a estes metais. [..]
description: Revista Captar: Ciência e Ambiente para Todos, vol. 6 n.º 1 (2016)
Finding meaningful participation in volunteer geographic information and citizen science: a case comparison in environmental application
Creators: name: Mu-Ning W. Brandeis
name: Zamanillo, Maria Isabel Carrera
Publisher: name: Taylor & Francis
Year: 2016
Subjects: subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Ecology
subject: FOS: Biological sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Marine Biology
subject: Cancer
subject: Science Policy

description: Volunteer geographic information (VGI) and citizen science (CS) have been used as a way to promote a form of democratization of knowledge. Crowdsourcing facilitates the massive collection of data through nonexpert volunteers. This study compares 57 VGI and CS projects through a cross-case comparison method to explore the level of involvement of participants, based on three main analytic dimensions (project rationale, actor interaction, and data flow) and 12 sub-variables. The primary goal of this research is to analyze the purpose and the role of technology in VGI and CS projects, using three matrices to explore emerging trade-offs. Finally, we synthesized the workflow of VGI and CS project and compared based on project purposes. At the end of this study, five main purposes were found among the selected projects, uncovering that data collection is the most common purpose. A general workflow can be conceptualized, but detail implementation will involve various trade-offs, especially between local involvement and large-area expert-oriented protocol implementations. By exploring diverse relationships among selected variables of analysis in VGI and CS projects, this study pretends to build a foundation for larger, global meta-analysis that can bring some transparency in the epistemic interactions between experts and nonexperts.
40 anos depois: a explosão de uma fábrica de produtos químicos que ainda afeta o ambiente
Creators: name: Fasola, Emmanuele
name: Scali, Stefano
name: Ferraguti, Marco
Publisher: name: Revista Captar: Ciência e Ambiente para Todos
Year: 2016

description: No dia 10 de julho de 1976 a explosão da fábrica ICMESA, no norte da Itália, gerou uma nuvem tóxica composta principalmente por 2,3,7,8-tetraclorodibenzo-p-dioxina (2,3,7,8-TCDD). O vento transportou os subprodutos em direção a uma área de 18,1 km2, contaminando pelo menos nove cidades. Cinco dias depois, os animais começaram a morrer e as pessoas tiveram a sua pele queimada com reações alérgicas. Nos anos seguintes, a taxa de doenças crônicas e agudas (por exemplo, pulmonares, cardio-vasculares e dermatológicas), nas áreas afetadas, aumentou drasticamente. Após uma campanha para remediação do solo nas áreas mais afetadas, este foi removido e os níveis de dioxina baixaram com o passar do tempo. Mas as características da dioxina (alto Poct/água e alto t1/2 no solo) permitem a sua persistência quase indefinidamente no ambiente. Esta situação pode levar a dioxina a ser transferida para a biota em indivíduos com risco de bioacumulação e biomagnificação através da cadeia trófica. Para investigar se os níveis atuais de dioxinas no solo ainda poderão afetar a biota, uma análise morfológica e imunológica foi conduzida em lagartos da espécie Podarcis muralis. Esta espécie pode ser encontrada em quase todos os tipos de habitat, primitivos e poluídos, pelo que a amostragem de indivíduos realizou-se ao longo de um gradiente de aumento da distância desde o local da explosão. Hemogramas (contagem de leucócitos) permitem inferir sobre as condições imunológicas dos indivíduos, enquanto a morfologia dos caracteres sexuais secundários dos machos permitem inferir sobre os efeitos de disrupção endócrina. Estes foram mé-todos não-invasivos e não destrutivos, que produziram indiretamente, estimativas suficientemente fiáveis de efeitos de depressão do sistema imunológico ou disrupção endócrina. Estas últimas análises não detetaram qualquer diferença estatística entre as populações. Embora os resultados das análises morfológicas mostrassem que os lagartos machos que viviam junto do local afetado sofreram feminização dos caracteres sexuais secundários. Seja ou não devido à dioxina libertada, a disrupção endócrina é provável, mas ainda precisa ser comprovada por mais investigações.
description: Revista Captar: Ciência e Ambiente para Todos, vol. 6 n.º 1 (2016)
A metformina reduz a fragmentação do DNA e a condensação imatura da cromatina em espermatozoides humanos in vitro
Creators: name: Ferreira, Carolina
name: Rabaça, Ana
name: Gonçalves, Ana
name: Barros, Alberto
name: Oliveira, Pedro F.
name: Sousa, Mário
name: Alves, Marco G.
name: Sá, Rosália
Publisher: name: Revista Captar: Ciência e Ambiente para Todos
Year: 2016

description: A metformina é um antidiabético oral da classe das biguanidas largamente prescrito para o tratamento da Diabetes mellitus tipo 2. A metformina tem sido utilizada em diversas patologias reprodutivas nomeadamente nas associadas com a resistência à insulina. Vários estudos avaliaram o efeito deste fármaco no sistema reprodutor masculino ao nível dos testículos, células de Sertoli e a sua capacidade antioxidante em espermatozoides de ratinho após criopreservação. Contudo, pouco se sabe acerca do seu efeito no aparelho reprodutor masculino adulto humano, particularmente em espermatozoides. O presente trabalho teve, portanto, como objetivo estudar este efeito in vitro.As amostras de sémen foram usadas após consentimento e os espermatozoides incubados na ausência e na presença de metformina (0, 5, 50 e 500 μM). A análise do sémen foi realizada de acordo com as diretrizes da Organização Mundial para a Saúde. A incidência de espermatozoides morfologicamente normais com fragmentação de DNA e condensação imatura da cromatina foi avaliada pelo ensaio TUNEL e pela técnica do azul de anilina, respetivamente. Após incubação com 50 μM de metformina verificou-se uma redução na percentagem de espermatozoides móveis. Doses mais baixas de metformina aumentaram a fragmentação do DNA e a condensação imatura da cromatina, ao passo que a concentração de 500 μM restaurou estes parâmetros para valores similares aos do grupo contro-lo. Perante estes resultados, a metformina apresenta efeitos benéficos importantes para os espermatozoides humanos durante a incubação, o que parece ser dependente da dose utilizada. Enquanto concentrações mais elevadas de metformina preservam a integridade do DNA, concentrações mais baixas são capazes de prevenir a redução da motilidade. Deste modo, a par das novas aplicações clínicas que têm sido atribuídas à metformina, propomos a sua utilização como aditivo ao meio de incubação dos espermatozoides, de forma a prevenir a degeneração e fragmentação do seu DNA.
description: Revista Captar: Ciência e Ambiente para Todos, vol. 6 n.º 1 (2016)
New planar trans-copper(II) β-dithioester chelate complexes: synthesis, characterization, anticancer activity and DNA-binding/cleavage studies
Creators: name: Yadav, Manoj Kumar
name: Akhilendra Kumar Maurya
name: Rajput, Gunjan
name: Manar, Krishna Kumar
name: Vinayak, Manjula
name: Drew, Michael G. B.
name: Nanhai Singh
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Biophysics
subject: Biochemistry
subject: Cell Biology
subject: Molecular Biology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Cancer
subject: 111714 Mental Health
subject: FOS: Health sciences

description: New planar trans-copper(II) β-dithioester complexes, [Cu(L)2] (L = methyl-3-hydroxy-(3-pyridyl)-2-propenedithioate (L1 in 1), methyl-3-hydroxy-(2-naphthyl)-2-propenedithioate (L2 in 2), methyl-3-hydroxy-3-(p-methoxyphenyl)-2-propenedithioate (L3 in 3), methyl-3-hydroxy-3-(p-fluorophenyl)-2-propenedithioate (L4 in 4), and methyl-3-hydroxy-3-(p-bromophenyl)-2-propenedithioate (L5 in 5)), have been synthesized and characterized by elemental (C, H, N and S) analysis, ESI-MS, IR, and UV-vis spectra. The structures of HL3 and its corresponding complex 3 have been determined by X-ray crystallography. Electrochemical behavior of all complexes has been studied by cyclic voltammetry. All five planar complexes show efficient DNA-binding and DNA (PBR322)-cleavage in a concentration-dependent manner (1 > 3 > 5 > 2 > 4). Cleavage efficiency is enhanced in the presence of H2O2 as well as ascorbic acid. However, the order of increased efficiency of Cu(II) chelates differs in the presence of H2O2 as 4 > 1 > 2 > 3 > 5. Among these complexes, the pyridyl- and methoxy-functionalized 1 and 3 have shown higher self-activating capability in DNA-cleavage. All complexes show significant variation in IC50 on MCF-7 cell line. Additionally, treatment with the complexes gradually increases apoptotic cell death in dose-dependent manner in RAW 264.7 cell line. These findings highlight potential cancer protective nature of these complexes.
U.S. Agricultural Colleges’ International Strategic Partnerships:
Creators: name: Lacy, William
name: Merilus, Jean-Yves
name: Liu, Xiaoguang
name: Lacy, Laura R.
Publisher: name: The International Journal of Sociology of Agriculture and Food
Year: 2022

description: Historically, U.S. colleges of agriculture have been leaders in generating, disseminating, and applying the latest science to address the needs of society, in educating the next generation of professionals and leaders, and in engaging in international partnerships. As these processes and goals become more global, strategic international partnerships become more critical. To understand how college leaders are approaching these critical relationships, deans of 33 leading U.S. colleges of agriculture were surveyed on key aspects of their international partnerships. The most frequently identified major goals were: ‘enhancing the quality of research and scholarship’; and ‘strengthening students’ education’. However, more than half the deans noted four challenging issues for realizing productive partnerships: ‘difference in educational quality and standards among partners’; ‘incongruent expectations between the institutions’; ‘language and cultural differences’; and ‘different levels of institutional commitment’. Fewer than half the deans indicated that dedicated resources were allocated for the partnerships. Ten critical topics often identified by college strategic plans were seen by most of the deans as important for their college but not nearly as important for their international partnerships. Three quarters of the deans identified seven factors for future successful international partnerships, including: ‘leadership at the program and college level’; and ‘adequate resources, including funding, eligible faculty, facilities and space’. Several future research needs were identified: international strategic partnerships will require informed and creative college leadership and likely need to expand in scale, scope, diversity and complexity, draw successfully on the scientific knowledge worldwide, and carefully consider the wide, unique opportunities and challenges of these partnerships.
description: The International Journal of Sociology of Agriculture and Food, Vol. 27 No. 2 (2021): Regular Issue
Presença de bactérias patogénicas e resistência antimicrobiana em ungulados selvagens portugueses
Creators: name: Dias, D.
name: Torres, R.
name: Fonseca, C.
name: Mendo, S.
name: Caetano, T.
Publisher: name: Revista Captar: Ciência e Ambiente para Todos
Year: 2016

description: De uma forma geral, os animais selvagens não estão expostos a agentes antimicrobianos. Assim, espera-se que a resistência da sua flora bacteriana a antibacterianos seja muito reduzida. Contudo, a crescente interação destes animais com atividades antropogénicas pode contribuir para um aumento da resistência das suas comunidades bacterianas. O presente estudo teve como objetivo: i) avaliar os níveis de resistência de isolados de E. coli e Salmonella spp. ii) avaliar o nível de ocorrência de estirpes de E. coli produtoras da toxina shiga (STEC). Para tal, foram recolhidas 67 amostras fecais das três espécies de ungulados com maior distribuição em Portugal: veado (n=42), javali (n=21) e corço (n=4), em três localizações geográficas distintas (Montesinho, Idanha-a-Nova e Lousã). Dez isolados de E. coli de cada amostra foram selecionados com base no seu crescimento em agar e amplificação dos genes uidA e gadA/B. Antes da realização dos testes de suscetibilidade a antibióticos, os isolados foram submetidos a BOX-PCR para selecionar estirpes geneticamente diferentes em cada amostra (n=152). Os resultados mostraram que 16,5% dos isolados de E. coli apresentam resistência a pelo menos um antibiótico, incluindo ampicilina (10%), tetraciclina (9%), streptomicina (5%), cotrimoxazol (4%), amoxicilina/ácido clavulânico (2%) e cefoxitina (1%), tendo em consideração os cut-offs clinicamente relevantes. Um fenótipo de multirresistência foi encontrado em 3% destes isolados, todos provenientes do centro de Portugal (Lousã). De acordo com os cut-offs epidemiológicos, 18% da população de E. coli analisada apresentou um fenótipo do tipo não-selvagem a pelo menos um antibiótico. No que se refere à ocorrência de Salmonella spp., os resultados indicam uma baixa prevalência (1,5%) deste agente etiológico na população de ungulados selvagens estudada, tendo sido apenas identificada num javali da região da Lousã. A pesquisa de STEC revelou que tanto veados como corços dos três locais estudados são portadores destas estirpes. Foram detetadas três variantes do gene stx (gene que codifica para a toxina shiga) nos isolados STEC, incluindo stx1c, stx2d e stx2g. Os resultados obtidos mostram que as populações de ungulados selvagens estudadas são reservatórios de bactérias resistentes, assim como de bactérias potencialmente patogénicas e podem, por isso, atuar como veículo de transmissão destas bactérias entre a vida selvagem, a pecuária e o Homem.
description: Revista Captar: Ciência e Ambiente para Todos, vol. 6 n.º 1 (2016)
Collaborating for Change
Creators: name: Zwart, Tjitske Anna
name: Lamers, Machiel
name: Mathijs, Erik
Publisher: name: The International Journal of Sociology of Agriculture and Food
Year: 2022

description: Worldwide, partnerships are popular vehicles for addressing (agro-food related) sustainability challenges. Their popularity is reflected in a proliferation of studies on partnerships. Yet, the current body of literature does not sufficiently take into account: (i) the importance of the institutional contexts in which the partnerships are embedded; and (ii) how interactions and tensions between the different partners influence the process of shaping the partnership over time. Therefore, in this paper, we aim to gain in-depth insights on processes of shaping a partnership for sustainable agriculture and food, and the role that the context in which a partnership is embedded plays in these processes. Our findings are based on the single case study of a participatory action research project of a partnership in Belgium. In our analysis, we take a social practices approach and thus conceptualise partnerships as different sets of practices from which a new set of practices is created. The results show that in talking about the sustainability of agriculture and food, it is important to take into account existing practice bundles because: (i) they set the scene in which sustainability innovations are shaped; and (ii) they create the boundaries for possible kinds of change. Participating in a new set of practices requires a move away from ‘normal’ ways of working. Yet, although there are shared goals and practices, each partner organisation is also constrained by and working towards its own goals. This is likewise the case for how ‘sustainability’ is shaped, implying that existing practices play an important role in shaping ‘sustainable’ practices. In turn, these findings highlight the need to not simply assume that partnerships will contribute to sustainability transitions, but also to reflect on: (i) whether and how this might be the case; and (ii) who or what factors have the power in shaping and defining ‘sustainability’.
description: The International Journal of Sociology of Agriculture and Food, Vol. 27 No. 2 (2021): Regular Issue
Innovation at the Margins: The Challenges for Bangladesh’s Aquaculture Hatchery Sector
Creators: name: Butcher, Andrea
name: Rahman, Muhammad Meezanur
name: Hinchliffe, Stephen J.
Publisher: name: The International Journal of Sociology of Agriculture and Food
Year: 2022

description: Reducing disease and improving quality control for shrimp and prawn production are key policy aims for Bangladesh’s export aquaculture with its hatchery sector, the foundation of aquaculture production, targeted for technical innovation. Hatchery reared postlarvae are carriage for the transferral of pathogens and potentially antibiotic resistant genes onto farms, compelling the Department of Fisheries to introduce the Hatchery Act in 2011, stipulating that all hatchery reared PL should be tested before onward sale. Policy implementation is achieved in collaboration with externally funded, locally managed development programmes for upgrading production management practices, delivering genetically enhanced breeds, and improving business margins by providing opportunities for pathogen-free guarantees and branding. However, uptake of improvements in the hatcheries has been low. Disease remains a key production challenge, and despite a reduction in antibiotic use, antibiotics remain a necessary component of disease control. In this paper, we develop a sociotechnical diagram, inspired by Actor Network Theory, that combines analytical models for innovation and disease outbreaks with a sociology of translation. Our approach highlights how vernacular trading practices, farmer financial and adaptive capacities, poor quality materials, and limited laboratory facilities tighten business margins and increase production risks, leaving hatcheries with little to no extra investment capacity or incentive to implement seed testing and improved management practices. We demonstrate the kinds of alliances to be made, interests to be negotiated, and adjustments to be considered for seed innovations to gain purchase in Bangladesh’s food production ecology.
description: The International Journal of Sociology of Agriculture and Food, Vol. 27 No. 2 (2021): Regular Issue
Cafeína e fertilidade masculina: efeitos metabólicos e perfil oxidativo em células de Sertoli humanas
Creators: name: Dias, Tânia
name: Alves, Marco
name: Bernardino, Raquel
name: Martins, Ana
name: Moreira, Carolina
name: Silva, Joaquina
name: Barros, Alberto
name: Sousa, Mário
name: Silva, Branca
name: Oliveira, Pedro
Publisher: name: Revista Captar: Ciência e Ambiente para Todos
Year: 2016

description: A cafeína é um componente comum do café, chá, bebidas energéticas, produtos que contenham cacau ou chocolate e até mesmo de vários medicamentos. O consumo excessivo de cafeína tem sido associado a diversos problemas de foro reprodutivo. Além disso, foi demonstrado que este composto tem uma ação moduladora no metabolismo celular. Isto levou-nos a colocar a hipótese de a cafeína poder alterar o metabolismo das células de Sertoli humanas (hSCs), as quais são essenciais para a espermatogénese e para a fertilidade masculina. Para verificar esta hipótese, as hSCs foram cultivadas na presença de cafeína (5, 50 e 500 μM) e o seu perfil glicolítico foi avaliado através do estudo do consumo de glucose e da produção de lactato e alanina. Os níveis de expressão proteica dos transportadores de glucose (GLUT1 e GLUT3), fosfofrutoquinase 1 (PFK1), lactato desidrogenase (LDH) e transportador de monocarboxilatos 4 (MCT4) foram também determinados, assim como a atividade da enzima LDH. Para além disso, como a cafeína demonstrou possuir propriedades antioxidantes, avaliámos a capacidade antioxidante das hSCs após a exposição à cafeína, assim como a formação de grupos carbonilo e a peroxidação lipídica. A cafeína nas concentrações mais baixas (5 e 50 μM) estimulou a produção de lactato, mas apenas as hSCs expostas a uma concentração de 50 μM apresentaram um aumento da expressão dos GLUTs. Na concentração mais elevada (500 μM), a atividade da LDH foi estimulada de forma a sustentar a produção de lactato. Notavelmente, a capacidade antioxidante das hSCs diminuiu de uma forma dose-dependente. Os nossos resultados indicam claramente que a exposição das hSCs à cafeína altera o seu metabolismo celular. Além da estimulação da produção de lactato nas hSCs expostas à cafeína, o qual é essencial para o desenvolvimento e sobrevivência das células germinativas, o perfil oxidativo destas células foi alterado. Adicionalmente, a concentração mais elevada de cafeína (500 μM) induziu um estado pró-oxidante, o que pode ser deletério para a progressão da espermatogénese, uma vez que esta normalmente já envolve níveis elevados de stress oxidativo. Mais estudos serão necessários para clarificar que doses de cafeína serão benéficas/prejudiciais para a função das hSCs, a fim de evitar problemas a nível da reprodução masculina.
description: Revista Captar: Ciência e Ambiente para Todos, vol. 6 n.º 1 (2016)
Prenatal exposure to tobacco smoke leads to increased mitochondrial DNA content in umbilical cord serum associated to reduced gestational age
Creators: name: Pirini, Francesca
name: Goldman, Lynn R.
name: Soudry, Ethan
name: Halden, Rolf U.
name: Witter, Frank
name: Sidransky, David
name: Guerrero-Preston, Rafael
Publisher: name: Taylor & Francis
Year: 2016
Subjects: subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Physiology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics
subject: Cancer
subject: 60506 Virology

description: We investigated if prenatal exposures to tobacco smoke lead to changes in mitochondrial DNA content (mtDNA) in cord serum and adversely affect newborns’ health. Umbilical cord serum cotinine levels were used to determine in utero exposure to smoking. Cord serum mtDNA was measured by quantitative polymerase chain reaction analysis of the genes coding for cytochrome c oxidase1 (MT-CO1) and cytochrome c oxidase2 (MT-CO2). Log transformed levels of mtDNA coding for MT-CO1 and MT-CO2 were significantly higher among infants of active smokers with higher serum level of cotinine (p p = 0.08; p = 0.02). Structural equation modeling results confirmed a positive association between cotinine and MT-CO1 and2 (p p = 0.02) and IGF-1 (p MT-CO1 and MT-CO2 associated to increased cord serum cotinine and decreased gestational age.
Data
Creators: name: Halim, Stefani Felita
Publisher: name: OSF Registries
Year: 2023

description: Loneliness adalah perasaan kekurangan dan ketidakpuasan yang dihasilkan oleh perbedaan antara hubungan sosial yang diinginkan dan jenis hubungan sosial yang dimiliki (Perlman & Peplau, 1981). Menurut Russell (1996), kesepian adalah hubungan sosial yang tidak sesuai dari apa yang diinginkan/dicapai. Self esteem merupakan sikap positif dan negatif individu terhadap diri sendiri (Rosenberg, 1965).
ВКЛАД ЖЕНЩИН В СПОРТИВНУЮ НАУКУ
Creators: name: Важенин, Р.Р.
name: Козаченко, А.Д.
name: Махинова, М.В.
Publisher: name: Zenodo
Year: 2024

description: В настоящей работе на основе научно-методической литературы рассмотрены вклады женщин в науке и спорте
Chromium(III), manganese(II) and iron(III) complexes based on hydrazone Schiff-base and azide ligands: Synthesis, crystal structure and antimicrobial activity
Creators: name: Shaabani, Behrouz
name: Khandar, Ali Akbar
name: Ramazani, Nahid
name: Fleck, Michel
name: Haedeh Mobaiyen
name: Cunha-Silva, Luís
Publisher: name: Taylor & Francis
Year: 2016
Subjects: subject: Biophysics
subject: Biochemistry
subject: 29999 Physical Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: Cell Biology
subject: Molecular Biology
subject: Pharmacology
subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences

description: A tridentate NNO donor hydrazine Schiff base, HL, was obtained from condensation of pyridine 2-carbaldehyde and 4-hydroxy benzohydrazide. HL and azide ligands with Cr(III), Mn(II) and Fe(III) have been used to synthesize [Cr(L)(N3)(OCH3)]2 (1), [Mn(HL)2(N3)2] (2) and [Fe(L)(N3)(OCH3)]2·H2O (3). HL is quite diverse in its chelating ability and can be a neutral or monoanionic ligand as a tridentate unit. In this paper, we report structures showing different denticities of the ligand having different charges. The ligand, 1, 2 and 3 were characterized by elemental analysis, FT-IR and UV–Vis spectral studies and solid-state structures were determined by single-crystal X-ray diffraction analysis, revealing that 1 and 3 are binuclear while 2 is mononuclear. The efficiencies of the ligand and the three complexes were evaluated for antimicrobial activity; MIC data revealed that HL, 1, 2 and 3 are not strongly active in comparison to standard drugs.
On the malleability of the meaning of contexts: the influence of another person’s emotion expressions on situation perception
Creators: name: Hess, Ursula
name: Hareli, Shlomo
Publisher: name: Taylor & Francis
Year: 2016
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Sociology
subject: FOS: Sociology
subject: Developmental Biology
subject: Science Policy
subject: 60506 Virology

description: Research on the relationship between context and facial expressions generally assumes a unidirectional effect of context on expressions. However, according to the model of the meaning of emotion expressions in context (MEEC) the effect should be bidirectional. The present research tested the effect of emotion expression on the interpretation of scenes. A total of 380 participants either (a) rated facial expressions with regard to the likely appraisal of the eliciting situation by the emoter, (b) appraised the scenes alone or (c) appraised scenes shown together with the expressions they supposedly elicited. The findings strongly supported the MEEC. When a scene was combined with an expression signalling a situation that is undesirable, or high in locus of control or sudden, the participants appraised the scene correspondingly. Thus, the meaning of scenes is malleable and affected by the way that people are seen to react to them.
model_final_randomscale_0.5_1_40000.pth
Creators: name: James, Edward
name: Wilson, Alex
name: Schwartz, Odelia
name: Pan, Xu
Publisher: name: figshare
Year: 2022
Subjects: subject: 60307 Host-Parasite Interactions
subject: FOS: Biological sciences

description: The .pth file for quantifying and phenotype Buchnera within confocal images of aphid bacteriocytes. Swapping this model with one trained in different systems would allow wider application of SymbiQuant.