title: Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð¿Ð°Ð½ÐµÐ»Ð¸ Ð¿ÑÐ°Ð¹Ð¼ÐµÑÐ¾Ð² Ð´Ð»Ñ ÑÐµÐ·Ð¸ÑÑÐ¾Ð¼Ð° Ð¾ÐºÑÑÐ¶Ð°ÑÑÐµÐ¹ ÑÑÐµÐ´Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Development of a panel of primers for the evaluation of the environmental resist
Creators: name: ÐÐ»Ð¸Ð¼Ð¾Ð²Ð°, ÐÐ»Ð¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Ð°Ð½ÑÐ¸Ð±Ð¸Ð¾ÑÐ¸ÐºÐ¾ÑÐµÐ·Ð¸ÑÑÐµÐ½ÑÐ½Ð¾ÑÑÑ
Klebsiella pneumoniae
Ð³ÐµÐ½Ñ ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾ÑÑÐ¸
Ð´Ð¸Ð·Ð°Ð¹Ð½ Ð¿ÑÐ°Ð¹Ð¼ÐµÑÐ¾Ð²
Ð¼ÑÐ»ÑÑÐ¸Ð¿Ð»ÐµÐºÑÐ½Ð°Ñ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð½Ð°Ñ ÑÐµÐ¿Ð½Ð°Ñ ÑÐµÐ°ÐºÑÐ¸Ñ
antibiotic resistance
resistance genes
primer design
multiplex polymerase chain reaction

description: ÐÐ°Ð½Ð½Ð°Ñ Ð½Ð°ÑÑÐ½Ð¾-Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÑÐºÐ°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð³ÐµÐ½Ð¾Ð² Ð°Ð½ÑÐ¸Ð±Ð¸Ð¾ÑÐ¸ÐºÐ¾ÑÐµÐ·Ð¸ÑÑÐµÐ½ÑÐ½Ð¾ÑÑÐ¸ Ñ Klebsiella pneumoniae Ð¸ ÑÐ¾Ð·Ð´Ð°Ð½Ð¸Ñ ÑÐµÑÐ¸Ð¸ ÑÐµÑÑ-ÑÐ¸ÑÑÐµÐ¼ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ Ð¼ÐµÑÐ¾Ð´Ð° Real-Time ÐÐ¦Ð  Ð² Ð¼ÑÐ»ÑÑÐ¸Ð¿Ð»ÐµÐºÑÐ½Ð¾Ð¹ ÑÐ¾ÑÐ¼Ð°ÑÐµ Ð´Ð»Ñ Ð´ÐµÑÐµÐºÑÐ¸Ð¸ ÑÑÐ¸Ñ ÑÐ¿Ð¸Ð´ÐµÐ¼Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸ Ð°ÐºÑÑÐ°Ð»ÑÐ½ÑÑ Ð³ÐµÐ½Ð¾Ð² Ð»ÐµÐºÐ°ÑÑÑÐ²ÐµÐ½Ð½Ð¾Ð¹ ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾ÑÑÐ¸ Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ Ð¼ÐµÐ´Ð¸ÑÐ¸Ð½ÑÐºÐ¾Ð¹ Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð¸ Ð¿ÑÐ¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ð¸ Ð¼Ð¾Ð½Ð¸ÑÐ¾ÑÐ¸Ð½Ð³Ð° Ð°Ð½ÑÑÐ¾Ð¿Ð¾Ð³ÐµÐ½Ð½ÑÑ Ð·Ð°Ð³ÑÑÐ·Ð½ÐµÐ½Ð¸Ð¹ Ð² Ð¿ÑÐ¸ÑÐ¾Ð´Ð½ÑÑ ÑÐºÐ¾ÑÐ¸ÑÑÐµÐ¼Ð°Ñ.ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: 1)Â ÐÑÑÐ°Ð±Ð¾ÑÐºÐ° Ð¼ÐµÑÐ¾Ð´Ð¸ÐºÐ¸ Ð¸Ð´ÐµÐ½ÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² ÑÐµÐ·Ð¸ÑÑÐµÐ½ÑÐ½Ð¾ÑÑÐ¸ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ ÐÐ¦Ð  Ñ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÐ·Ð½ÑÐ¼ ÑÐ¾ÑÐ¼Ð°ÑÐ¾Ð¼ Ð´ÐµÑÐµÐºÑÐ¸Ð¸; 2)Â Ð¤Ð¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÐºÐ¾Ð»Ð»ÐµÐºÑÐ¸Ð¸ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½ÑÑ Ð¾Ð±ÑÐ°Ð·ÑÐ¾Ð² Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð¹ ÐÐÐ, ÑÐ¾Ð´ÐµÑÐ¶Ð°ÑÐµÐ¹ Ð³ÐµÐ½Ñ ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾ÑÑÐ¸ Ðº Ð°Ð½ÑÐ¸Ð±Ð¸Ð¾ÑÐ¸ÐºÐ°Ð¼; 3)Â ÐÐ´ÐµÐ½ÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ñ ÐºÐ¾Ð½ÑÐµÑÐ²Ð°ÑÐ¸Ð²Ð½ÑÑ ÑÑÐ°ÑÑÐºÐ¾Ð² Ð³ÐµÐ½Ð¾Ð² Ð¸ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð´Ð¸Ð·Ð°Ð¹Ð½Ð° Ð¿ÑÐ°Ð¹Ð¼ÐµÑÐ¾Ð² Ð´Ð»Ñ Ð¼ÑÐ»ÑÑÐ¸Ð¿Ð»ÐµÐºÑÐ½Ð¾Ð¹ Real-Time ÐÐ¦Ð , Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÑÑÐ¸Ð¹ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÑÑ Ð´ÐµÑÐµÐºÑÐ¸Ñ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÐµÐ¹ Ð´Ð°Ð½Ð½ÑÑ Ð³ÐµÐ½Ð¾Ð²; 4)Â ÐÐ¿ÑÐ¾Ð±Ð°ÑÐ¸Ñ Ð¼ÑÐ»ÑÑÐ¸Ð¿Ð»ÐµÐºÑÐ½Ð¾Ð¹ Real-Time ÐÐ¦Ð  Ð½Ð° ÐºÐ¾Ð»Ð»ÐµÐºÑÐ¸Ð¸ ÐÐÐ ÐºÐ»Ð¸Ð½Ð¸ÑÐµÑÐºÐ¸Ñ Ð¼ÑÐ»ÑÑÐ¸Ð°Ð½ÑÐ¸Ð±Ð¸Ð¾ÑÐ¸ÐºÐ¾ÑÐµÐ·Ð¸ÑÑÐµÐ½ÑÐ½ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð² Klebsiella pneumoniae. ÐÐ±ÑÐµÐºÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð¼Ð¾Ð´ÐµÐ»ÑÐ½Ð¾Ð³Ð¾ Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð° Ð´Ð»Ñ Ð¾ÑÑÐ°Ð±Ð¾ÑÐºÐ¸ ÑÐµÑÑ-Ð¿Ð°Ð½ÐµÐ»Ð¸ Ð±ÑÐ»Ð¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ ÑÑÐ°Ð¼Ð¼Ñ K. pneumoniae, Ð½Ð° ÐºÐ¾ÑÐ¾ÑÑÑ Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»Ð°ÑÑ Ð°Ð¿ÑÐ¾Ð±Ð°ÑÐ¸Ñ Ð¼ÑÐ»ÑÑÐ¸Ð¿Ð»ÐµÐºÑÐ½Ð¾Ð¹ Real-Time ÐÐ¦Ð  Ð½Ð° Ð½Ð°Ð»Ð¸ÑÐ¸Ðµ Ð°ÐºÑÑÐ°Ð»ÑÐ½ÑÑ Ð³ÐµÐ½Ð¾Ð² Ð»ÐµÐºÐ°ÑÑÑÐ²ÐµÐ½Ð½Ð¾Ð¹ ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾ÑÑÐ¸.Â ÐÐ°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ½ÑÐµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»Ð¸ÑÑ Ð² Ð¼Ð¸ÐºÑÐ¾Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð¸ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾-Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ ÐºÐ°ÑÐµÐ´ÑÑ ÑÐ¿Ð¸Ð´ÐµÐ¼Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ð¸, Ð¿Ð°ÑÐ°Ð·Ð¸ÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¸ Ð´ÐµÐ·Ð¸Ð½ÑÐµÐºÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¡ÐÐÐÐ£ Ð¸Ð¼. Ð. Ð. ÐÐµÑÐ½Ð¸ÐºÐ¾Ð²Ð°. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¸ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ñ Ð³ÐµÐ½Ñ Ð°Ð½ÑÐ¸Ð±Ð¸Ð¾ÑÐ¸ÐºÐ¾ÑÐµÐ·Ð¸ÑÑÐµÐ½ÑÐ½Ð¾ÑÑÐ¸ Ð²Ð¾ Ð²ÑÐµÑ Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼ÑÑ Ð¾Ð±ÑÐ°Ð·ÑÐ°Ñ, Ð¿ÑÐµÐ´Ð»Ð¾Ð¶ÐµÐ½ Ð´Ð¸Ð·Ð°Ð¹Ð½ Ð¿ÑÐ°Ð¹Ð¼ÐµÑÐ¾Ð² Ð´Ð»Ñ ÐºÐ¾Ð½ÑÐµÑÐ²Ð°ÑÐ¸Ð²Ð½ÑÑ ÑÑÐ°ÑÑÐºÐ¾Ð² Ð³ÐµÐ½Ð¾Ð² Ð¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð¼ÑÐ»ÑÑÐ¸Ð¿Ð»ÐµÐºÑÐ½Ð°Ñ Real-Time Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð½Ð°Ñ ÑÐµÐ¿Ð½Ð°Ñ ÑÐµÐ°ÐºÑÐ¸Ñ Ð½Ð° ÐºÐ¾Ð»Ð»ÐµÐºÑÐ¸Ð¸ ÐÐÐ ÐºÐ»Ð¸Ð½Ð¸ÑÐµÑÐºÐ¸Ñ Ð¼ÑÐ»ÑÑÐ¸Ð°Ð½ÑÐ¸Ð±Ð¸Ð¾ÑÐ¸ÐºÐ¾ÑÐµÐ·Ð¸ÑÑÐµÐ½ÑÐ½ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð² Klebsiella pneumoniae.
description: This research work is devoted to the study of antibiotic resistance genes in Klebsiella pneumoniae and the creation of a series of test systems based on the Real-Time PCR method in a multiplex format for the detection of these epidemiologically relevant drug resistance genes in a medical organization and when monitoring anthropogenic pollution in ecosystems.Tasks that were solved during the study: 1) Development of a methodology for identifying resistance genes using PCR with an electrophoretic detection format; 2) Formation of a collection of control samples of bacterial DNA containing genes for resistance to antibiotics; 3)Â Identification of conservative regions of genes and development of primer design for multiplex Real-Time PCR, which allows detection of these gene sequences; 4) Approbation of multiplex Real-Time PCR on the DNA collection of clinical multi-antibiotic resistant strains of Klebsiella pneumoniae.Â The object of the study. K. pneumoniae strains were used as a model organism for testing the test panel, on which multiplex Real-Time PCR was tested for the presence of relevant drug resistance genes. Laboratory studies were carried out in the microbiological and molecular genetic laboratory of the Department of Epidemiology, Parasitology and Disinfectology of the North-Western State Medical University. I. I. Mechnikov. As a result of the study, antibiotic resistance genes were found in all studied samples, a primer design for conservative gene regions was proposed, and a multiplex Real-Time polymerase chain reaction was carried out on DNA collections of clinical multi-antibiotic-resistant strains of Klebsiella pneumoniae.