title: ÐÐ¾Ð»ÑÑÐµÐ½Ð¸Ðµ Ð¸ Ñ Ð°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ° ÑÐµÐºÐ¾Ð¼Ð±Ð¸Ð½Ð°Ð·Ð½Ð¾Ð¹ Ð½ÑÐºÐ»ÐµÐ°Ð·Ñ LBCAS12A
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Obtaining and characteristics of recombinase nuclease LBCAS12A
Creators: name: Ð¢ÑÑÐ²Ð°ÑÑÐ»Ð»Ð¸Ð½, Ð ÑÑÐ»Ð°Ð½
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: ÑÐµÐºÐ¾Ð¼Ð±Ð¸Ð½Ð°Ð·Ð½Ð°Ñ Ð½ÑÐºÐ»ÐµÐ°Ð·Ð° LBCAS12A
Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ðµ Ð±ÐµÐ»ÐºÐ°
Ð¿ÑÐ¾Ð²ÐµÑÐºÐ° Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð½Ð° ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐ½Ð¾ÑÑÑ
ÑÐµÐ´Ð°ÐºÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð³ÐµÐ½Ð¾Ð¼Ð°
LBCAS12A recombinase nuclease
protein production
activity testing for specificity
genome editing

description: Ð Ð¿Ð¾ÑÐ»ÐµÐ´Ð½ÐµÐµ Ð²ÑÐµÐ¼Ñ Ð¿Ð¾ÑÐ²Ð»ÑÐµÑÑÑ Ð²ÑÐµ Ð±Ð¾Ð»ÑÑÐµ Ð½Ð°ÑÑÐ½ÑÑ ÑÐ°Ð±Ð¾Ñ, Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð½ÑÑ ÑÐ¸ÑÑÐµÐ¼Ðµ CRISPR/Cas â ÑÐ°ÑÑÐ¸ Ð°Ð´Ð°Ð¿ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ Ð¸Ð¼Ð¼ÑÐ½Ð¸ÑÐµÑÐ° Ð¿ÑÐ¾ÐºÐ°ÑÐ¸Ð¾Ñ, ÐºÐ¾ÑÐ¾ÑÑÑ Ð±ÑÐ»Ð° Ð¿ÑÐ¸ÑÐ¿Ð¾ÑÐ¾Ð±Ð»ÐµÐ½Ð° Ð² ÑÑÑÐµÐºÑÐ¸Ð²Ð½ÑÐ¹ Ð¸Ð½ÑÑÑÑÐ¼ÐµÐ½Ñ ÑÐµÐ´Ð°ÐºÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð³ÐµÐ½Ð¾Ð¼Ð°. ÐÐ°Ð½Ð½Ð°Ñ ÑÐ¸ÑÑÐµÐ¼Ð° Ð²ÐºÐ»ÑÑÐ°ÐµÑ Ð¼Ð°ÑÑÐ¸Ð² CRISPR, Ð²ÑÑÑÐ¾ÐµÐ½Ð½ÑÐ¹ Ð² Ð³ÐµÐ½Ð¾Ð¼ Ð°ÑÑÐµÐ¸ Ð¸Ð»Ð¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð¸, Ð² ÐºÐ¾ÑÐ¾ÑÐ¾Ð¼ ÑÑÐ°Ð½Ð¸ÑÑÑ Ð¸Ð½ÑÐ¾ÑÐ¼Ð°ÑÐ¸Ñ Ð¾ Ð³ÐµÐ½Ð¾Ð¼Ðµ Ð¿Ð°ÑÐ¾Ð³ÐµÐ½Ð½ÑÑ ÑÐ°Ð³Ð¾Ð², Ð° ÑÐ°ÐºÐ¶Ðµ Ð³ÐµÐ½Ñ, Ð¾ÑÐ²ÐµÑÐ°ÑÑÐ¸Ðµ Ð·Ð° Cas-Ð±ÐµÐ»ÐºÐ¸. ÐÑÐ¸ÑÐ¸Ð½Ð° Ð¿Ð¾Ð¿ÑÐ»ÑÑÐ½Ð¾ÑÑÐ¸ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ñ ÑÐ²Ð»ÑÐµÑÑÑ Ð² Ð¿ÑÐ¾ÑÑÐ¾ÑÐµ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ñ, Ð²ÑÑÐ¾ÐºÐ°Ñ ÑÐ¾ÑÐ½Ð¾ÑÑÑ, Ð¾ÑÐ½Ð¾ÑÐ¸ÑÐµÐ»ÑÐ½Ð°Ñ Ð»ÐµÐ³ÐºÐ¾ÑÑÑ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ Ð² Ð±Ð¸Ð¾ÑÐ¸Ð¼Ð¸ÑÐµÑÐºÐ¸Ñ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ½ÑÑ ÑÑÐ»Ð¾Ð²Ð¸ÑÑ. ÐÐ»Ð°Ð²Ð½Ð°Ñ Ð¿ÑÐ¸ÑÐ¸Ð½Ð° Ð¼Ð½Ð¾Ð³Ð¸Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ â ÑÑÐ¾ Ð±ÑÐ´ÑÑÐ¸Ðµ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ CRISPR/Cas Ð² Ð¼ÐµÐ´Ð¸ÑÐ¸Ð½Ðµ Ð¸ Ð±Ð¸Ð¾ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¸, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ðµ Ð½Ð¾Ð²ÑÑ Ð²Ð¸Ð´Ð¾Ð² ÑÐ°ÑÑÐµÐ½Ð¸Ð¹ Ð¸ Ð¶Ð¸Ð²Ð¾ÑÐ½ÑÑ, Ð¿Ð¾Ð»ÐµÐ·Ð½ÑÑ Ð² ÑÐµÐ»ÑÑÐºÐ¾Ð¼ ÑÐ¾Ð·ÑÐ¹ÑÑÐ²Ðµ, Ð²ÑÐ²ÐµÐ´ÐµÐ½Ð¸Ðµ Ð½Ð¾Ð²ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð² Ð±Ð°ÐºÑÐµÑÐ¸Ð¹, ÑÐ¿Ð¾ÑÐ¾Ð±Ð½ÑÑ Ð²ÑÑÐ°Ð±Ð°ÑÑÐ²Ð°ÑÑ Ð² Ð±Ð¾Ð»ÑÑÐ¾Ð¼ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ðµ Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼ÑÐµ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÑ. ÐÐºÑÑÐ°Ð»ÑÐ½Ð¾ÑÑÑ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ Ð·Ð°ÐºÐ»ÑÑÐ°ÐµÑÑÑ Ð² Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¸ Ð±ÐµÐ»ÐºÐ° Cas12a, ÐºÐ¾ÑÐ¾ÑÑÐ¹ ÑÐ²Ð»ÑÐµÑÑÑ Ð¿ÐµÑÑÐ¿ÐµÐºÑÐ¸Ð²Ð½ÑÐ¼ Ñ ÑÐ¾ÑÐºÐ¸ Ð·ÑÐµÐ½Ð¸Ñ Ð³ÐµÐ½Ð½Ð¾Ð¹ Ð¸Ð½Ð¶ÐµÐ½ÐµÑÐ¸Ð¸. ÐÐ°Ð½Ð½ÑÐ¹ ÑÐµÑÐ¼ÐµÐ½Ñ Ð¿Ð¾Ð´Ð°ÐµÑ Ð±Ð¾Ð»ÑÑÐ¸Ðµ Ð½Ð°Ð´ÐµÐ¶Ð´Ñ Ð¸Ð·-Ð·Ð° ÑÐ²Ð¾ÐµÐ¹ ÑÐ¾ÑÐ½Ð¾ÑÑÐ¸ Ð¸ ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐ½Ð¾ÑÑÐ¸. ÐÐ°Ð¼ ÑÐ´Ð°Ð»Ð¾ÑÑ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°ÑÑ Ð¿ÑÐ¾ÑÐ¾ÐºÐ¾Ð» Ð´Ð»Ñ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ Ð´Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð¹ ÑÑÐ°Ð½ÑÑÐ¾ÑÐ¼Ð°ÑÐ¸Ð¸, Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÑ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ Ð¿Ð¾Ð´ÑÐ¾Ð´ÑÑÐ¸Ðµ Ð±ÑÑÐµÑÐ½ÑÐµ ÑÐ¸ÑÑÐµÐ¼Ñ. ÐÑ Ð¿ÑÐ¾Ð²ÐµÐ»Ð¸ ÑÐµÑÑ Ð½Ð° ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐ½Ð¾ÑÑÑ, Ð² ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ ÐºÐ¾ÑÐ¾ÑÐ¾Ð³Ð¾ Ð·Ð°Ð´Ð°Ð½Ð½ÑÐµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° Ð±ÐµÐ»ÐºÐ° Ð¿Ð¾Ð´ÑÐ²ÐµÑÐ´Ð¸Ð»Ð¸ÑÑ, ÑÑÐ¾ ÑÐ²Ð¸Ð´ÐµÑÐµÐ»ÑÑÑÐ²ÑÐµÑ Ð¾ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾Ð¼ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¸ LbCas12a ÐºÐ°Ðº ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ Ð¸Ð½ÑÑÑÑÐ¼ÐµÐ½ÑÐ° ÑÐµÐ´Ð°ÐºÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð³ÐµÐ½Ð¾Ð¼Ð°.
description: Recently, more and more scientific papers have appeared on the CRISPR / Cas system, this is a part of the adaptive immunity of prokaryotes, which has been adapted into an effective tool for genome editing. This system includes a CRISPR array built into the genome of archaea or bacteria, which stores information about the genome of pathogenic phages, as well as the genes responsible for Cas proteins. The reason for the popularity of this system is its ease of use, high accuracy, and relative ease of production in biochemical laboratory conditions. The main reason for many studies is the future use of CRISPR / Cas in medicine and biotechnology, the production of new species of plants and animals useful in agriculture, the breeding of new strains of bacteria capable of producing the necessary drugs in large quantities. The relevance of this work lies in the study of the Cas12a protein, which is promising from the point of view of genetic engineering. This enzyme is promising due to its precision and specificity. We were able to develop a protocol for obtaining this protein by the method of bacterial transformation, to investigate the most suitable buffer systems. We conducted a specificity test, as a result of which the specified properties of the protein were confirmed, which indicates the possible use of LbCas12a as an effective tool for genome editing.