title: ÐÐ´ÐµÐ½ÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ñ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ Bifidobacterium longum Ð² ÐºÐ¸ÑÐµÑÐ½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾Ð±Ð¸Ð¾ÑÐµ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð½Ð¾Ð¹ ÑÐµÐ¿Ð½Ð¾Ð¹ ÑÐµÐ°ÐºÑÐ¸Ð¸ Ð² ÑÐµÐ¶Ð¸Ð¼Ðµ ÑÐµÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð²ÑÐµÐ¼ÐµÐ½Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Identification of Bifidobacterium longum bacteria in the human intestinal microbiota using apolymerase chain reaction in real time
Creators: name: Ð¡Ð°Ð²ÐµÐ»ÑÐµÐ²Ð°, ÐÐ»Ð¸Ð·Ð°Ð²ÐµÑÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: ÐÐ¸Ð¾ÑÐµÑ Ð½Ð¾Ð»Ð¾Ð³Ð¸Ñ
ÐÐ°ÐºÑÐµÑÐ¸Ð¸
Ð¿ÑÑ Ð² ÑÐµÐ¶Ð¸Ð¼Ðµ ÑÐµÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð²ÑÐµÐ¼ÐµÐ½Ð¸
Ð¾Ð»Ð¸Ð³Ð¾Ð½ÑÐºÐ»ÐµÐ¾ÑÐ¸Ð´Ð½ÑÐµ Ð¿ÑÐ°Ð¹Ð¼ÐµÑÑ
Ð¾Ð¿ÑÐ¸Ð¼Ð°Ð»ÑÐ½ÑÐµ ÑÑÐ»Ð¾Ð²Ð¸Ñ Ð¿ÑÑ
ÐºÐ¸ÑÐµÑÐ½Ð°Ñ Ð¼Ð¸ÐºÑÐ¾Ð±Ð¸Ð¾ÑÐ°
bifidobacterium longum
real-time pcr
selection of oligonucleotide primers and probe
selection of optimal conditions for pcr
intestinal microbiota

description: ÐÐ°Ð´Ð°ÑÐ¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: 1. ÐÐ¾Ð´Ð¾Ð±ÑÐ°ÑÑ Ð¾Ð»Ð¸Ð³Ð¾Ð½ÑÐºÐ»ÐµÐ¾ÑÐ¸Ð´Ð½ÑÐµ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÐ¸ Ð¿ÑÐ°Ð¹Ð¼ÐµÑÐ¾Ð² Ð¸ Ð·Ð¾Ð½Ð´Ð° Ð´Ð»Ñ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐ¸ Bifidobacterium longum. 2. ÐÐ¿ÑÐ¸Ð¼Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°ÑÑ ÑÑÐ»Ð¾Ð²Ð¸Ñ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð½Ð¾Ð¹ ÑÐµÐ¿Ð½Ð¾Ð¹ ÑÐµÐ°ÐºÑÐ¸Ð¸ Ð² ÑÐµÐ¶Ð¸Ð¼Ðµ ÑÐµÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð²ÑÐµÐ¼ÐµÐ½Ð¸ Ð´Ð»Ñ Ð¸Ð´ÐµÐ½ÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ Bifidobacterium longum. ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ñ Ð½Ð° Ð±Ð°Ð·Ðµ ÐÐÐ Â«ÐÐ»ÑÑÐ°Ð»Ð°Ð±Â» (Ð¡ÐÐ±). ÐÑÐ»Ð¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ñ ÑÐ°ÑÑÐµÑÑ, Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÑÑÑÐ¸Ðµ Ð½Ð°Ð»Ð¸ÑÐ¸Ðµ Ð¸ ÑÐ°ÑÑÐ¾ÑÑ Ð²ÑÑÑÐµÑÐ°ÐµÐ¼Ð¾ÑÑÐ¸ Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼Ð¾Ð³Ð¾ ÑÑÐ°ÑÑÐºÐ° Ð³ÐµÐ½Ð° Ð² Ð³ÐµÐ½Ð¾ÑÐ¸Ð¿Ðµ Bifidobacterium longum, Ð¸ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÑÐ°Ð±Ð¾ÑÑ Ð¿Ð¾Ð´Ð¾Ð±ÑÐ°Ð½Ð½ÑÑ Ð¿ÑÐ°Ð¹Ð¼ÐµÑÐ¾Ð² Ð¸ Ð·Ð¾Ð½Ð´Ð°. ÐÐ½Ð°Ð»Ð¸Ð· Ð³ÐµÐ½Ð¾ÑÐ¸Ð¿Ð° Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»ÑÑ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð¼Ð°ÑÐµÐ¼Ð°ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¼Ð¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ð½Ð¾Ð³Ð¾ Ð¾Ð±ÐµÑÐ¿ÐµÑÐµÐ½Ð¸Ñ Unipro Ugene, Ð´Ð»Ñ Ð¿Ð¾Ð´Ð±Ð¾ÑÐ° Ð¿ÑÐ°Ð¹Ð¼ÐµÑÐ¾Ð² Ð¸ Ð·Ð¾Ð½Ð´Ð° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»ÑÑ Ð¸Ð½ÑÐµÑÐ½ÐµÑ ÑÐµÑÑÑÑ PrimerQuest. Ð­ÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÑÐ°ÑÑÑÐ¸ÑÑÐ²Ð°Ð»Ð°ÑÑ Ð¿Ð¾ ÑÐ¾ÑÐ¼ÑÐ»Ðµ, Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ð½Ð¾Ð³Ð¾ Ð¾Ð±ÐµÑÐ¿ÐµÑÐµÐ½Ñ Excel. ÐÑÑÑÐµÑÑÐ²Ð»ÐµÐ½Ñ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ½ÑÐµ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÑ Ð¿Ð¾ Ð¿Ð¾Ð´Ð±Ð¾ÑÑ Ð¾Ð¿ÑÐ¸Ð¼Ð°Ð»ÑÐ½ÑÑ ÑÑÐ»Ð¾Ð²Ð¸Ð¹ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ ÐÐ¦Ð  Ð² ÑÐµÐ¶Ð¸Ð¼Ðµ ÑÐµÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð²ÑÐµÐ¼ÐµÐ½Ð¸. Ð ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ Ð±ÑÐ»Ð¸ Ð¿Ð¾Ð´Ð¾Ð±ÑÐ°Ð½Ñ Ð¾Ð»Ð¸Ð³Ð¾Ð½ÑÐºÐ»ÐµÐ¾ÑÐ¸Ð´Ð½ÑÐµ Ð¿ÑÐ°Ð¹Ð¼ÐµÑÑ Ð¸ Ð·Ð¾Ð½Ð´, Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ñ Ð¸ ÑÐµÐºÐ¾Ð¼ÐµÐ½Ð´Ð¾Ð²Ð°Ð½Ñ Ð¾Ð¿ÑÐ¸Ð¼Ð°Ð»ÑÐ½ÑÐµ ÑÑÐ»Ð¾Ð²Ð¸Ñ Ð´Ð»Ñ ÐÐ¦Ð  Ð² ÑÐµÐ¶Ð¸Ð¼Ðµ ÑÐµÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð²ÑÐµÐ¼ÐµÐ½Ð¸ Ð´Ð»Ñ Ð¸Ð´ÐµÐ½ÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Bifidobacterium longum Ð² Ð¼Ð¸ÐºÑÐ¾Ð±Ð¸Ð¾ÑÐµ ÐºÐ¸ÑÐµÑÐ½Ð¸ÐºÐ°.
description: The research set the following goals: 1. To select the oligonucleotide sequences of primers and probe for the diagnosis of Bifidobacterium longum. 2. Optimize the conditions of the polymerase chain reaction in real time for theidentification of Bifidobacterium longum bacteria. The work was fulfilled on the premises of LLC âAlfalabâ, where laboratory experiments were conducted to select optimal conditions for real-time PCR. Calculations were performed to determine the presence and frequency of occurrence of the studied gene site in the genotype of Bifidobacterium longum, andthe effectiveness of the selected primers and probe. The genotype analysis was carried out by mathematical modeling using the Unipro Ugene software, and the PrimerQuest Internet resource was used to select primers and a probe. The efficiency was calculated using a formula using Excel software. As a result, oligonucleotide primers and a probe were selected to identify Bifidobacterium longum in the intestinal microbiota, optimal conditions for realtime PCR were investigated and recommended.