title: ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¿ÑÐ¾ÑÐµÑÑÐ¾Ð² Ð°Ð½Ð¸Ð·Ð¾ÑÑÐ¾Ð¿Ð½Ð¾Ð¹ Ð±ÐµÐ·ÑÐ·Ð»ÑÑÐ°ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÐµÐ»Ð°ÐºÑÐ°ÑÐ¸Ð¸ Ð² Ð²Ð¾Ð´Ð½Ð¾-ÑÐ¿Ð¸ÑÑÐ¾Ð²ÑÑ ÑÐ°ÑÑÐ²Ð¾ÑÐ°Ñ NADH Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð½Ð°ÐºÐ°ÑÐºÐ°-Ð·Ð¾Ð½Ð´Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Study of anisotropic relaxation of NADH in methanol and propylene glycol solution
Creators: name: ÐÐ¾Ð»ÐºÐ¾Ð², ÐÐµÐ½Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Ð Ð°ÑÑÐ²Ð¾ÑÑ
Ð Ð°ÑÑÐ²Ð¾ÑÑ Ð²Ð¾Ð´Ð½ÑÐµ
ÐÐµÑÐ¸Ð»Ð¾Ð²ÑÐ¹ ÑÐ¿Ð¸ÑÑ
Ð¡Ð¿ÐµÐºÑÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ
Ð¼ÐµÑÐ¾Ð´ Ð¿Ð¾Ð»ÑÑÐ¸Ð·Ð°ÑÐ¸Ð¾Ð½Ð½Ð¾-Ð¼Ð¾Ð´ÑÐ»ÑÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ¿ÐµÐºÑÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸ Ð½Ð°ÐºÐ°ÑÐºÐ°-Ð·Ð¾Ð½Ð´Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
Ð¿ÑÐ¾Ð¿Ð¸Ð»ÐµÐ½Ð³Ð»Ð¸ÐºÐ¾Ð»Ñ
Ð°Ð½Ð¸Ð·Ð¾ÑÑÐ¾Ð¿Ð½Ð°Ñ ÐºÐ¾Ð»ÐµÐ±Ð°ÑÐµÐ»ÑÐ½Ð°Ñ ÑÐµÐ»Ð°ÐºÑÐ°ÑÐ¸Ñ
Ð²ÑÐµÐ¼Ñ Ð°Ð½Ð¸Ð·Ð¾ÑÑÐ¾Ð¿Ð½Ð¾Ð¹ ÐºÐ¾Ð»ÐµÐ±Ð°ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÐµÐ»Ð°ÐºÑÐ°ÑÐ¸Ð¸
ÑÑÐ¸Ð¼ÑÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ðµ Ð Ð°Ð¼Ð°Ð½Ð¾Ð²ÑÐºÐ¾Ðµ ÑÐ°ÑÑÐµÑÐ½Ð¸Ðµ
polarization-modulation pump-probe transient spectroscopy
propylene glycol
anisotropic relaxation
anisotropy relaxation time
stimulated Raman scattering

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¿ÑÐ¾ÑÐµÑÑÐ¾Ð² Ð°Ð½Ð¸Ð·Ð¾ÑÑÐ¾Ð¿Ð½Ð¾Ð¹ ÐºÐ¾Ð»ÐµÐ±Ð°ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÐµÐ»Ð°ÐºÑÐ°ÑÐ¸Ð¸ Ð¼Ð¾Ð»ÐµÐºÑÐ» Ð½Ð¸ÐºÐ¾ÑÐ¸Ð½Ð°Ð¼Ð¸Ð´-Ð°Ð´ÐµÐ½Ð¸Ð½-Ð´Ð¸Ð½ÑÐºÐ»ÐµÐ¾ÑÐ¸Ð´ (NADH) Ð² Ð²Ð¾Ð´Ð½Ð¾-ÑÐ¿Ð¸ÑÑÐ¾Ð²ÑÑ ÑÐ°ÑÑÐ²Ð¾ÑÐ°Ñ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð½ÐµÐ´Ð°Ð²Ð½Ð¾ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð½Ð¾Ð³Ð¾ Ð¼ÐµÑÐ¾Ð´Ð° Ð¿Ð¾Ð»ÑÑÐ¸Ð·Ð°ÑÐ¸Ð¾Ð½Ð½Ð¾-Ð¼Ð¾Ð´ÑÐ»ÑÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ¿ÐµÐºÑÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸ Ð½Ð°ÐºÐ°ÑÐºÐ°-Ð·Ð¾Ð½Ð´Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ. ÐÐµÑÐ¾Ð´ Ð¾ÑÐ½Ð¾Ð²Ð°Ð½ Ð½Ð° Ð²ÑÑÐ¾ÐºÐ¾ÑÐ°ÑÑÐ¾ÑÐ½Ð¾Ð¹ Ð¼Ð¾Ð´ÑÐ»ÑÑÐ¸Ð¸ Ð¿Ð¾Ð»ÑÑÐ¸Ð·Ð°ÑÐ¸Ð¸ Ð¸Ð·Ð»ÑÑÐµÐ½Ð¸Ñ Ð½Ð°ÐºÐ°ÑÐºÐ¸ Ð¸ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐµÐ³Ð¾ Ð½Ð°Ð±Ð»ÑÐ´ÐµÐ½Ð¸Ñ ÑÐ°Ð·Ð½Ð¾ÑÑÐ½Ð¾Ð³Ð¾ Ð¿Ð¾Ð³Ð»Ð¾ÑÐµÐ½Ð¸Ñ Ð¾ÑÑÐ¾Ð³Ð¾Ð½Ð°Ð»ÑÐ½ÑÑ ÐºÐ¾Ð¼Ð¿Ð¾Ð½ÐµÐ½Ñ Ð¿Ð¾Ð»ÑÑÐ¸Ð·Ð°ÑÐ¸Ð¸ Ð·Ð¾Ð½Ð´Ð¸ÑÑÑÑÐµÐ³Ð¾ Ð¿ÑÑÐºÐ° Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð±Ð°Ð»Ð°Ð½ÑÐ½Ð¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ñ Ð´ÐµÑÐµÐºÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¸ ÑÐ¸Ð½ÑÑÐ¾Ð½Ð½Ð¾Ð³Ð¾ ÑÑÐ¸Ð»Ð¸ÑÐµÐ»Ñ. ÐÑÑ Ð½Ð°ÐºÐ°ÑÐºÐ¸ Ñ Ð´Ð»Ð¸Ð½Ð¾Ð¹ Ð²Ð¾Ð»Ð½Ñ 360 Ð½Ð¼ Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÐµÑ Ð¿ÐµÑÐµÐ²Ð¾Ð´Ð¸ÑÑ Ð¼Ð¾Ð»ÐµÐºÑÐ»Ñ NADH Ð² Ð¿ÐµÑÐ²Ð¾Ðµ Ð²Ð¾Ð·Ð±ÑÐ¶Ð´ÐµÐ½Ð½Ð¾Ðµ ÑÐ¾ÑÑÐ¾ÑÐ½Ð¸Ðµ, Ð° Ð·Ð¾Ð½Ð´Ð¸ÑÑÑÑÐµÐµ Ð¸Ð·Ð»ÑÑÐµÐ½Ð¸Ðµ Ñ Ð´Ð»Ð¸Ð½Ð¾Ð¹ Ð²Ð¾Ð»Ð½Ñ 720 Ð½Ð¼ Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÐµÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÑ Ð¿ÑÐ¾ÑÐµÑÑÑ Ð°Ð½Ð¸Ð·Ð¾ÑÑÐ¾Ð¿Ð½Ð¾Ð¹ ÐºÐ¾Ð»ÐµÐ±Ð°ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÐµÐ»Ð°ÐºÑÐ°ÑÐ¸Ð¸ ÑÑÐ¾Ð³Ð¾ ÑÐ¾ÑÑÐ¾ÑÐ½Ð¸Ñ. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ° Ð¸ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÐ¸ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ ÑÐ¸Ð³Ð½Ð°Ð»Ð¾Ð², Ð±ÑÐ»Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ñ Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾ÑÑÐ¸ Ð²ÑÐµÐ¼ÐµÐ½Ð¸ Ð°Ð½Ð¸Ð·Ð¾ÑÑÐ¾Ð¿Ð½Ð¾Ð¹ ÐºÐ¾Ð»ÐµÐ±Ð°ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÐµÐ»Ð°ÐºÑÐ°ÑÐ¸Ð¸ Ïv Ð¸ ÐºÐ¾ÑÑÑÐ¸ÑÐ¸ÐµÐ½ÑÐ¾Ð² A1 Ð¸ A0 Ð¾Ñ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ ÑÐ¿Ð¸ÑÑÐ° Ð² ÑÐ°ÑÑÐ²Ð¾ÑÐµ. ÐÐ°Ð²Ð¸ÑÐ¸Ð¼Ð¾ÑÑÑ Ïv Ð¾Ñ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ Ð¼ÐµÑÐ°Ð½Ð¾Ð»Ð° Ð¾ÐºÐ°Ð·Ð°Ð»Ð°ÑÑ Ð»Ð¸Ð½ÐµÐ¹Ð½Ð¾Ð¹ Ð¸ Ð²Ð¾Ð·ÑÐ°ÑÑÐ°ÑÑÐµÐ¹, ÑÑÐ¾ ÑÐ²ÑÐ·Ð°Ð½Ð¾ Ñ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸ÐµÐ¼ Ð¿Ð¾Ð»ÑÑÐ½Ð¾ÑÑÐ¸ ÑÐ°ÑÑÐ²Ð¾ÑÐ¸ÑÐµÐ»Ñ. ÐÐ°Ð²Ð¸ÑÐ¸Ð¼Ð¾ÑÑÑ Ïv Ð¾Ñ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ Ð¿ÑÐ¾Ð¿Ð¸Ð»ÐµÐ½Ð³Ð»Ð¸ÐºÐ¾Ð»Ñ Ð² Ð¼ÐµÑÐ°Ð½Ð¾Ð»Ðµ Ð±ÑÐ»Ð° Ð½ÐµÐ»Ð¸Ð½ÐµÐ¹Ð½Ð¾Ð¹ Ð¸ Ð²Ð¾Ð·ÑÐ°ÑÑÐ°ÑÑÐµÐ¹, ÑÑÐ¾ ÑÐ²ÑÐ·Ð°Ð½Ð¾ Ñ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸ÐµÐ¼ Ð²ÑÐ·ÐºÐ¾ÑÑÐ¸ ÑÐ°ÑÑÐ²Ð¾ÑÐ¸ÑÐµÐ»Ñ. ÐÑÐµÐ¼ÐµÐ½Ð° Ð°Ð½Ð¸Ð·Ð¾ÑÑÐ¾Ð¿Ð½Ð¾Ð¹ ÑÐµÐ»Ð°ÐºÑÐ°ÑÐ¸Ð¸ NADH ÑÐ¾ÑÑÐ°Ð²Ð¸Ð»Ð¸: Ð² Ð²Ð¾Ð´Ðµ Ïv = 1.4 Ð¿Ñ, Ð² Ð¼ÐµÑÐ°Ð½Ð¾Ð»Ðµ Ïv = 8 Ð¿Ñ, Ð² Ð¿ÑÐ¾Ð¿Ð¸Ð»ÐµÐ½Ð³Ð»Ð¸ÐºÐ¾Ð»Ðµ Ïv = 66 Ð¿Ñ. ÐÐ¾ÑÑÑÐ¸ÑÐ¸ÐµÐ½ÑÑ A1 Ð¸ A0 Ð»Ð¸Ð½ÐµÐ¹Ð½Ð¾ ÑÐ±ÑÐ²Ð°Ð»Ð¸ Ð¾Ñ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ Ð¼ÐµÑÐ°Ð½Ð¾Ð»Ð°. Ð Ð² ÑÐ¼ÐµÑÐ¸ Ð¿ÑÐ¾Ð¿Ð¸Ð»ÐµÐ½Ð³Ð»Ð¸ÐºÐ¾Ð»Ñ-Ð¼ÐµÑÐ°Ð½Ð¾Ð» Ð¿Ð¾Ð²ÐµÐ´ÐµÐ½Ð¸Ðµ ÑÑÐ¸Ñ ÐºÐ¾ÑÑÑÐ¸ÑÐ¸ÐµÐ½ÑÐ¾Ð² Ð¾ÑÐ»Ð¸ÑÐ°Ð»Ð¾ÑÑ. Ð ÐµÐ·ÑÐ»ÑÑÐ°ÑÑ ÑÐ°Ð±Ð¾ÑÑ Ð¸Ð¼ÐµÑÑ Ð²Ð°Ð¶Ð½Ð¾Ðµ Ð·Ð½Ð°ÑÐµÐ½Ð¸Ðµ Ð´Ð»Ñ ÑÐ¿ÐµÐºÑÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸ Ð¸ Ð¼Ð¾Ð³ÑÑ Ð±ÑÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ Ð´Ð»Ñ Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐµÐ³Ð¾ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ñ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² ÑÐµÐ¼ÑÐ¾ÑÐ¸Ð¼Ð¸Ð¸ Ð¸ Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð¿ÑÐ¾ÑÐµÑÑÐ¾Ð² ÑÐµÐ»Ð°ÐºÑÐ°ÑÐ¸Ð¸ Ð²Ð¾Ð·Ð±ÑÐ¶Ð´ÐµÐ½Ð½ÑÑ ÑÐ¾ÑÑÐ¾ÑÐ½Ð¸Ð¹.
description: The given work is devoted to the study of the processes of anisotropic vibrational relaxation of nicotinamide-adenine-dinucleotide (NADH) molecules in aqueous-alcohol solutions by the recently developed polarization-modulation pump-probe transient spectroscopy. The method is based on modulation of the polarizationÂ  of the pump beam and observation of the difference absorption of the orthogonalÂ  components of the polarization of the probe beam using a balanced detection system and a lock-in amplifier. The wavelength of the pump beam allows transfer of NADH molecules to the first excited state, and the probe beam wavelength makes it possible to study processes of anisotropic vibrational relaxation of this state. As a result of the experiments and the processing of experimental signals, the dependences of the anisotropic vibrational relaxation time tv and the coefficients A1, A0 on the alcohol concentration in the solution were obtained. The dependence of tv on the concentration of methanol turned out to be linear and increasing, which is associated with a change in the polarity of the solvent. The dependence of tv on the propylene glycol concentration in the methanol was nonlinear and increasing, which is associated with a change in the viscosity of the solvent. The anisotropic relaxation times of NADH were in water Ïv = 1.4 ps, in methanol Ïv = 8 ps, in propylene glycol Ïv = 66 ps. the coefficients A1 and A0 decreased linearly with the concentration of methanol increasing. In a solution of propylene glycol and methanol, A1 is nonlinear to concentrations of propylene glycol. The results of the work are important for spectroscopy and can be used for further development of methods of Femtochemistry and study of relaxation processes of excited states.