cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Additional file 1 of Using structural equation modeling to investigate students’ satisfaction with an undergraduate tutorial system
Creators: name: Wu, Xiaorong
name: Liu, Hui
name: Zhang, Cong
name: Zhang, Fangda
name: Xie, Biao
name: Zhong, Xiaoni
Publisher: name: figshare
Year: 2024
Subjects: subject: Biotechnology
subject: Ecology
subject: FOS: Biological sciences
subject: Sociology
subject: FOS: Sociology
subject: Biological Sciences not elsewhere classified
subject: Science Policy

description: Supplementary Material 1
Obesity alters endoxifen plasma levels in young breast cancer patients: A pharmacometric simulation approach
Creators: name: Mueller‐Schoell, Anna
name: Klopp‐Schulze, Lena
name: Michelet, Robin
name: Kloft, Charlotte
name: Schroth, Werner
name: Mürdter, Thomas
name: Brauch, Hiltrud
name: Huisinga, Wilhelm
name: Joerger, Markus
name: Neven, Patrick
Publisher: name: Freie Universität Berlin
Year: 2020
Subjects: subject: obesity
subject: breast cancer
subject: endoxifen plasma
subject: 500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::572 Biochemie

description: Endoxifen is one of the most important metabolites of the prodrug tamoxifen. High interindividual variability in endoxifen steady‐state concentrations (CSS,min ENDX) is observed under tamoxifen standard dosing and patients with breast cancer who do not reach endoxifen concentrations above a proposed therapeutic threshold of 5.97 ng/mL may be at a 26% higher recurrence risk compared with patients with endoxifen concentrations exceeding this value. In this investigation, 10 clinical tamoxifen studies were pooled (1,388 patients) to investigate influential factors on CSS,min ENDX using nonlinear mixed‐effects modeling. Age and body weight were found to significantly impact CSS,min ENDX in addition to CYP2D6 phenotype. Compared with postmenopausal patients, premenopausal patients had a 30% higher risk for subtarget CSS,min ENDX at tamoxifen 20 mg per day. In treatment simulations for distinct patient subpopulations, young overweight patients had a 3.1–13.8‐fold higher risk for subtarget CSS,min ENDX compared with elderly low‐weight patients. Considering ever‐rising obesity rates and the clinical importance of tamoxifen for premenopausal patients, this subpopulation may benefit most from individualized tamoxifen dosing.
A π‐Conjugated, Covalent Phosphinine Framework
Creators: name: Huang, Jieyang
name: Tarábek, Ján
name: Kulkarni, Ranjit
name: Wang, Cui
name: Dračínský, Martin
name: Smales, Glen J.
name: Tian, Yu
name: Ren, Shijie
name: Pauw, Brian R.
name: Resch‐Genger, Ute
Publisher: name: Freie Universität Berlin
Year: 2019
Subjects: subject: fluorescence
subject: phosphinine
subject: polymers
subject: Suzuki–Miyaura coupling
subject: π-conjugated frameworks
subject: 500 Naturwissenschaften und Mathematik::540 Chemie::540 Chemie und zugeordnete Wissenschaften

description: Structural modularity of polymer frameworks is a key advantage of covalent organic polymers, however, only C, N, O, Si, and S have found their way into their building blocks so far. Here, the toolbox available to polymer and materials chemists is expanded by one additional nonmetal, phosphorus. Starting with a building block that contains a λ5‐phosphinine (C5P) moiety, a number of polymerization protocols are evaluated, finally obtaining a π‐conjugated, covalent phosphinine‐based framework (CPF‐1) through Suzuki–Miyaura coupling. CPF‐1 is a weakly porous polymer glass (72.4 m2 g−1 BET at 77 K) with green fluorescence (λmax=546 nm) and extremely high thermal stability. The polymer catalyzes hydrogen evolution from water under UV and visible light irradiation without the need for additional co‐catalyst at a rate of 33.3 μmol h−1 g−1. These results demonstrate for the first time the incorporation of the phosphinine motif into a complex polymer framework. Phosphinine‐based frameworks show promising electronic and optical properties, which might spark future interest in their applications in light‐emitting devices and heterogeneous catalysis.
Potential energy function for a photo‐switchable lipid molecule
Creators: name: Klaja, Oskar
name: Frank, James A.
name: Trauner, Dirk
name: Bondar, Ana-Nicoleta
Publisher: name: Freie Universität Berlin
Year: 2020
Subjects: subject: cis‐azobenzene
subject: force‐field
subject: photo‐switchable lipids
subject: potential energy function
subject: trans‐azobenzene
subject: 500 Naturwissenschaften und Mathematik::530 Physik::530 Physik
subject: 500 Naturwissenschaften und Mathematik::540 Chemie::540 Chemie und zugeordnete Wissenschaften

description: Photo‐switchable lipids are synthetic lipid molecules used in photo‐pharmacology to alter membrane lateral pressure and thus control opening and closing of mechanosensitive ion channels. The molecular picture of how photo‐switchable lipids interact with membranes or ion channels is poorly understood. To facilitate all‐atom simulations that could provide a molecular picture of membranes with photo‐switchable lipids, we derived force field parameters for atomistic computations of the azobenzene‐based fatty acid FAAzo‐4. We implemented a Phyton‐based algorithm to make the optimization of atomic partial charges more efficient. Overall, the parameters we derived give good description of the equilibrium structure, torsional properties, and non‐bonded interactions for the photo‐switchable lipid in its trans and cis intermediate states, and crystal lattice parameters for trans‐FAAzo‐4. These parameters can be extended to all‐atom descriptions of various photo‐switchable lipids that have an azobenzene moiety.
Additional file 1 of Predictive modeling of postoperative gastrointestinal dysfunction: the role of serum bilirubin, sodium levels, and surgical duration in gynecological cancer care
Creators: name: He, Lijuan
name: Hu, Jun
name: Han, Yun
name: Xiong, Wenli
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Cell Biology
subject: Physiology
subject: FOS: Biological sciences
subject: Biological Sciences not elsewhere classified
subject: Mathematical Sciences not elsewhere classified
subject: Science Policy

description: Additional file 1: Supplemental Fig. 1. Comprehensive Visualization of Key Dataset Variables.
sj-docx-1-heb-10.1177_10901981231188136 – Supplemental material for Development and Evaluation of a Blended Self-Management Program for Metabolic Syndrome in Patients With Rheumatic Diseases
Creators: name: Im, BoAe
name: Oh, HyunSoo
name: Kim, SooHyun
name: Jeong, HyeSun
name: Seo, WhaSook
Publisher: name: SAGE Journals
Year: 2023
Subjects: subject: Health and Community Services
subject: Psychology not elsewhere classified
subject: Psychiatry (incl. Psychotherapy)
subject: Public Health and Health Services not elsewhere classified
subject: Sociology
subject: FOS: Sociology

description: Supplemental material, sj-docx-1-heb-10.1177_10901981231188136 for Development and Evaluation of a Blended Self-Management Program for Metabolic Syndrome in Patients With Rheumatic Diseases by BoAe Im, HyunSoo Oh, SooHyun Kim, HyeSun Jeong and WhaSook Seo in Health Education & Behavior
The GFZ GRACE RL06 monthly gravity field time series: Processing details and quality assessment
Creators: name: Dahle, Christoph
name: Sulzbach, Roman
name: Murböck, Michael
name: Flechtner, Frank
name: Dobslaw, Henryk
name: Michalak, Grzegorz
name: Neumayer, Karl Hans
name: Abrykosov, Oleh
name: Reinhold, Anton
name: König, Rolf
name: Förste, Christoph
Publisher: name: Freie Universität Berlin
Year: 2019
Subjects: subject: satellite gravimetry
subject: GRACE
subject: Level-2 processing
subject: time-variable gravity field
subject: mass change monitoring
subject: 500 Naturwissenschaften und Mathematik::550 Geowissenschaften, Geologie::551 Geologie, Hydrologie, Meteorologie

description: Time-variable gravity field models derived from observations of the Gravity Recovery and Climate Experiment (GRACE) mission, whose science operations phase ended in June 2017 after more than 15 years, enabled a multitude of studies of Earth’s surface mass transport processes and climate change. The German Research Centre for Geosciences (GFZ), routinely processing such monthly gravity fields as part of the GRACE Science Data System, has reprocessed the complete GRACE mission and released an improved GFZ GRACE RL06 monthly gravity field time series. This study provides an insight into the processing strategy of GFZ RL06 which has been considerably changed with respect to previous GFZ GRACE releases, and modifications relative to the precursor GFZ RL05a are described. The quality of the RL06 gravity field models is analyzed and discussed both in the spectral and spatial domain in comparison to the RL05a time series. All results indicate significant improvements of about 40% in terms of reduced noise. It is also shown that the GFZ RL06 time series is a step forward in terms of consistency, and that errors of the gravity field coefficients are more realistic. These findings are confirmed as well by independent validation of the monthly GRACE models, as done in this work by means of ocean bottom pressure in situ observations and orbit tests with the GOCE satellite. Thus, the GFZ GRACE RL06 time series allows for a better quantification of mass changes in the Earth system.
An essential thioredoxin-type protein of Trypanosoma brucei acts as redox-regulated mitochondrial chaperone
Creators: name: Currier, Rachel B.
name: Antelmann, Haike
name: Ulrich, Kathrin
name: Leroux, Alejandro E.
name: Dirdjaja, Natalie
name: Deambrosi, Matías
name: Bonilla, Mariana
name: Ahmed, Yasar Luqman
name: Adrian, Lorenz
name: Jakob, Ursula
Publisher: name: Freie Universität Berlin
Year: 2019
Subjects: subject: Trypanosoma brucei gambiense
subject: luciferase
subject: RNA interference
subject: parasitic diseases
subject: Trypanosoma
subject: mitochondria
subject: recombinant proteins
subject: cysteine
subject: 500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::579 Mikroorganismen, Pilze, Algen

description: Most known thioredoxin-type proteins (Trx) participate in redox pathways, using two highly conserved cysteine residues to catalyze thiol-disulfide exchange reactions. Here we demonstrate that the so far unexplored Trx2 from African trypanosomes (Trypanosoma brucei) lacks protein disulfide reductase activity but functions as an effective temperature-activated and redox-regulated chaperone. Immunofluorescence microscopy and fractionated cell lysis revealed that Trx2 is located in the mitochondrion of the parasite. RNA-interference and gene knock-out approaches showed that depletion of Trx2 impairs growth of both mammalian bloodstream and insect stage procyclic parasites. Procyclic cells lacking Trx2 stop proliferation under standard culture conditions at 27°C and are unable to survive prolonged exposure to 37°C, indicating that Trx2 plays a vital role that becomes augmented under heat stress. Moreover, we found that Trx2 contributes to the in vivo infectivity of T. brucei. Remarkably, a Trx2 version, in which all five cysteines were replaced by serine residues, complements for the wildtype protein in conditional knock-out cells and confers parasite infectivity in the mouse model. Characterization of the recombinant protein revealed that Trx2 can coordinate an iron sulfur cluster and is highly sensitive towards spontaneous oxidation. Moreover, we discovered that both wildtype and mutant Trx2 protect other proteins against thermal aggregation and preserve their ability to refold upon return to non-stress conditions. Activation of the chaperone function of Trx2 appears to be triggered by temperature-mediated structural changes and inhibited by oxidative disulfide bond formation. Our studies indicate that Trx2 acts as a novel chaperone in the unique single mitochondrion of T. brucei and reveal a new perspective regarding the physiological function of thioredoxin-type proteins in trypanosomes.
title: Βιώσιμες εναλλακτικές πηγές πρωτεϊνών τελευταίας γενιάς: Μέθοδοι επεξεργασίας και ιδιότητες για εφαρμογή σε τρόφιμα
title: State-of-the-art sustainable alternative protein sources: Processing paths and properties for food applications
titleType: AlternativeTitle
Creators: name: Κυριάκου, Χριστίνα
Publisher: name: Πανεπιστήμιο Δυτικής Αττικής
Year: 2024
Subjects: Εναλλακτικές πρωτεΐνες
Καλλιεργημένο κρέας
Φυτικές πρωτεΐνες
Πρωτεΐνες από έντομα
Μονοκυτταρική πρωτεΐνη

description: Η ασφάλεια των τροφίμων και τα περιβαλλοντικά προβλήματα έχουν εξελιχθεί σε παγκόσμιες κρίσεις που απαιτούν ριζικές λύσεις. Ο παγκόσμιος πληθυσμός αναμένεται να φτάσει τα 9,8 δισεκατομμύρια μέχρι το 2050, και η πλειονότητα αυτών των ανθρώπων θα ζει σε αναπτυσσόμενες χώρες της Αφρικής και της Ασίας. Η αυξανόμενη κατανάλωση κρέατος σε παγκόσμιο επίπεδο συνδέεται με αρνητικές περιβαλλοντικές επιπτώσεις, όπως η αύξηση των εκπομπών αερίων του θερμοκηπίου, που συμβάλλουν στην υπερθέρμανση του πλανήτη, καθώς και η αυξημένη χρήση νερού και γης. Καθώς η παραγωγή ζωικών προϊόντων γίνεται ολοένα και λιγότερο βιώσιμη, είναι απαραίτητο να βρεθούν γρήγορα εναλλακτικές πηγές πρωτεΐνης. Οι εναλλακτικές πρωτεΐνες αποτελούν «λύση» στην επιστήμη των τροφίμων. Αυτές προέρχονται από καλλιεργημένο κρέας, φυτικές πηγές, έντομα και από μονοκύτταρους οργανισμούς. Ειδικότερα, στην παρούσα εργασία γίνεται ανασκόπηση γύρω από τις ζωικής μορφής πρωτεϊνών, και πως έχει «αναγκαστεί» η επιστήμη των τροφίμων να δημιουργήσει νέες μορφές εναλλακτικών πηγών πρωτεΐνης με απώτερο σκοπό ένα βιώσιμο περιβάλλον. Επιπλέον, παρουσιάζονται Κανονισμοί και Νομοθεσίες που έχουν τεθεί σε ισχύ μετά από έρευνες, σε συνδυασμό με την βιωσιμότητα και τη μειωμένη εκπομπή αερίων του θερμοκηπίου, συγκριτικά με την παραγωγή ζωικών προϊόντων όπως είναι γνωστή έως και σήμερα. Τέλος, γίνεται αναφορά στη προτίμηση των καταναλωτών, η οποία απαιτεί περεταίρω έρευνα σχετικά με τα κριτήρια αλλά και την ηθική πλευρά του κάθε ατόμου για την αγορά ή όχι, προϊόντων που περιέχουν «καλλιεργημένο κρέας», φυτικές πρωτεΐνες, πρωτεΐνες από έντομα ή από μονοκύτταρους οργανισμούς.
Additional file 3 of Ameliorating the drought stress tolerance of a susceptible soybean cultivar, MAUS 2 through dual inoculation with selected rhizobia and AM fungus
Creators: name: Ashwin, Revanna
name: Bagyaraj, Davis Joseph
name: Mohan Raju, Basavaiah
Publisher: name: figshare
Year: 2024
Subjects: subject: Plant Biology
subject: FOS: Biological sciences

description: Additional file 3: Fig. S3. Comparison of leaf growth in soybean cultivar, MAUS 2 inoculated with Ambispora leptoticha + Bradyrhizobium liaoningense grown under irrigated and moisture stressed field conditions.
title: Διερεύνηση του εγκλήματος στον κυβερνοχώρο: Διερεύνηση των τεχνικών και τεχνολογιών που χρησιμοποιούνται στις έρευνες εγκλήματος στον κυβερνοχώρο, όπως η ψηφιακή εγκληματολογία, η ανάλυση δικτύων και η κοινωνική μηχανική
title: Investigating cybercrime: Exploring the techniques and technologies used in cybercrime investigations, such as digital forensics, network analysis, and social engineering
titleType: AlternativeTitle
Creators: name: Ιωάννης, Ατσάλης
Publisher: name: Πανεπιστήμιο Δυτικής Αττικής
Year: 2024
Subjects: Κυβερνοασφάλεια
Κοινωνική μηχανική
Ψηφιακή εγκληματολογία
Ανάλυση κυκλοφορίας δικτύων

description: Η παρούσα διπλωματική εργασία ασχολείται με τη διερεύνηση εγκλήματος στον κυβερνοχώρο, εστιάζοντας στις τεχνικές και τεχνολογίες που χρησιμοποιούνται για την ανίχνευση, ανάλυση και πρόληψη ψηφιακών επιθέσεων. Το περιεχόμενο της εργασίας καλύπτει τρεις βασικούς τομείς: την ψηφιακή εγκληματολογία, την ανάλυση δικτύων και την κοινωνική μηχανική. Η ψηφιακή εγκληματολογία αναλύει τις μεθόδους συλλογής, διατήρησης και ανάλυσης ψηφιακών αποδεικτικών στοιχείων, ενώ εξετάζει τις προκλήσεις της ανάκτησης χαμένων δεδομένων και την ανάλυση αρχείων καταγραφής συστημάτων για τον εντοπισμό κακόβουλων δραστηριοτήτων. Η ανάλυση δικτύων εστιάζει στον εντοπισμό και την ανάλυση της κυκλοφορίας των δεδομένων στα δίκτυα, ώστε να αναγνωριστούν οι διαδρομές και οι πηγές των κυβερνοεπιθέσεων. Η ανάλυση αυτή είναι κρίσιμη για την κατανόηση των μεθόδων που χρησιμοποιούν οι επιτιθέμενοι και την εφαρμογή βέλτιστων πρακτικών κυβερνοασφάλειας. Η κοινωνική μηχανική εξετάζει τις ψυχολογικές στρατηγικές που χρησιμοποιούν οι επιτιθέμενοι για να χειραγωγήσουν άτομα, με στόχο την απόκτηση μη εξουσιοδοτημένης πρόσβασης σε πληροφορίες ή συστήματα. Μέσα από την ανάλυση τεχνικών όπως το phishing, αναδεικνύεται η σημασία της εκπαίδευσης και ευαισθητοποίησης για την πρόληψη αυτών των επιθέσεων. Συμπερασματικά, η παρούσα διατριβή παρέχει μια ολοκληρωμένη διερεύνηση των τεχνικών και τεχνολογιών που στηρίζουν τις έρευνες για την αντιμετώπιση του εγκλήματος στον κυβερνοχώρο. Μέσα από μια εις βάθος ανάλυση της ψηφιακής εγκληματολογίας, της ανάλυσης δικτύων και της κοινωνικής μηχανικής, υπογραμμίζεται η σημασία μιας πολύπλευρης προσέγγισης για την καταπολέμηση των απειλών στον κυβερνοχώρο. Καθώς το ψηφιακό τοπίο συνεχίζει να εξελίσσεται, τα ευρήματά μου τονίζουν την ανάγκη συνεχούς εκπαίδευσης, συνεργασίας και ενσωμάτωσης προηγμένων εργαλείων για την αποτελεσματική αντιμετώπιση των προκλήσεων που τίθενται από τα εγκλήματα στον κυβερνοχώρο. Με τη διάδοση αυτής της γνώσης, συμβάλλουμε σε έναν ασφαλέστερο ψηφιακό κόσμο για άτομα, οργανισμούς και κοινωνίες γενικότερα.
sj-docx-3-jia-10.1177_23259582221101811 - Supplemental material for Depression and Anxiety in Youth and Young Adults Living with HIV: Frequency and Associated Factors in Thai Setting
Creators: name: Chantaratin, Sasitorn
name: Trimetha, Kawita
name: Werarak, Peerawong
name: Lapphra, Keswadee
name: Maleesatharn, Alan
name: Rungmaitree, Supattra
name: Wittawatmongkol, Orasri
name: Phongsamart, Wanatpreeya
name: Kongstan, Nantaka
name: Khumcha, Benjawan
name: Chokephaibulkit, Kulkanya
Publisher: name: SAGE Journals
Year: 2022
Subjects: subject: 111708 Health and Community Services
subject: FOS: Health sciences
subject: 111702 Aged Health Care
subject: 111799 Public Health and Health Services not elsewhere classified

description: Supplemental material, sj-docx-3-jia-10.1177_23259582221101811 for Depression and Anxiety in Youth and Young Adults Living with HIV: Frequency and Associated Factors in Thai Setting by Sasitorn Chantaratin, Kawita Trimetha, Peerawong Werarak, Keswadee Lapphra, Alan Maleesatharn, Supattra Rungmaitree, Orasri Wittawatmongkol, Wanatpreeya Phongsamart, Nantaka Kongstan, Benjawan Khumcha and Kulkanya Chokephaibulkit in Journal of the International Association of Providers of AIDS Care (JIAPAC)
Retracing the molecular basis and evolutionary history of the loss of benzaldehyde emission in the genus Capsella
Creators: name: Jantzen, Friederike
name: Höfflin, Jona
name: Hilker, Monika
name: Lynch, Joseph H.
name: Kappel, Christian
name: Skaliter, Oded
name: Wozniak, Natalia
name: Sicard, Adrien
name: Sas, Claudia
name: Adebesin, Funmilayo
Publisher: name: Freie Universität Berlin
Year: 2019
Subjects: subject: benzaldehyde
subject: Capsella
subject: cinnamate‐CoA ligase
subject: evolution
subject: floral scent
subject: selfing syndrome
subject: shepherd's purse
subject: 500 Naturwissenschaften und Mathematik::590 Tiere (Zoologie)::590 Tiere (Zoologie)

description: The transition from pollinator‐mediated outbreeding to selfing has occurred many times in angiosperms. This is generally accompanied by a reduction in traits attracting pollinators, including reduced emission of floral scent. In Capsella, emission of benzaldehyde as a main component of floral scent has been lost in selfing C. rubella by mutation of cinnamate‐CoA ligase CNL1. However, the biochemical basis and evolutionary history of this loss remain unknown, as does the reason for the absence of benzaldehyde emission in the independently derived selfer Capsella orientalis. We used plant transformation, in vitro enzyme assays, population genetics and quantitative genetics to address these questions. CNL1 has been inactivated twice independently by point mutations in C. rubella, causing a loss of enzymatic activity. Both inactive haplotypes are found within and outside of Greece, the centre of origin of C. rubella, indicating that they arose before its geographical spread. By contrast, the loss of benzaldehyde emission in C. orientalis is not due to an inactivating mutation in CNL1. CNL1 represents a hotspot for mutations that eliminate benzaldehyde emission, potentially reflecting the limited pleiotropy and large effect of its inactivation. Nevertheless, even closely related species have followed different evolutionary routes in reducing floral scent.
Dual-scattering near-field microscope for correlative nanoimaging of SERS and electromagnetic hotspots
Creators: name: Kusch, Patryk
name: Mastel, Stefan
name: Mueller, Niclas S.
name: Azpiazu, Nieves Morquillas
name: Heeg, Sebastian
name: Gorbachev, Roman
name: Schedin, Fredrik
name: Hübner, Uwe
name: Pascual, Jose I.
name: Reich, Stephanie
Publisher: name: Freie Universität Berlin
Year: 2017
Subjects: subject: SERS
subject: s-SNOM
subject: Raman scattering
subject: plasmon
subject: hotspot mapping
subject: 500 Naturwissenschaften und Mathematik::530 Physik::530 Physik

description: Surface-enhanced Raman spectroscopy (SERS) enables sensitive chemical studies and materials identification, relying on electromagnetic (EM) and chemical-enhancement mechanisms. Here we introduce a tool for the correlative nanoimaging of EM and SERS hotspots, areas of strongly enhanced EM fields and Raman scattering, respectively. To that end, we implemented a grating spectrometer into a scattering-type scanning near-field optical microscope (s-SNOM) for mapping of both the elastically and inelastically (Raman) scattered light from the near-field probe, that is, a sharp silicon tip. With plasmon-resonant gold dimers (canonical SERS substrates) we demonstrate with nanoscale spatial resolution that the enhanced Raman scattering from the tip is strongly correlated with its enhanced elastic scattering, the latter providing access to the EM-field enhancement at the illumination frequency. Our technique has wide application potential in the correlative nanoimaging of local-field enhancement and SERS efficiency as well as in the investigation and quality control of novel SERS substrates.
Additional file 1 of Ameliorating the drought stress tolerance of a susceptible soybean cultivar, MAUS 2 through dual inoculation with selected rhizobia and AM fungus
Creators: name: Ashwin, Revanna
name: Bagyaraj, Davis Joseph
name: Mohan Raju, Basavaiah
Publisher: name: figshare
Year: 2024
Subjects: subject: Plant Biology
subject: FOS: Biological sciences

description: Additional file 1: Fig. S1. Influence of dual inoculation on a soil moisture, b soil water potential and c soil temperature of study plots analyzed at flowering and pod filling stage in a drought susceptible soybean cultivar, MAUS 2 grown under irrigated and moisture stressed field conditions. Dual inoculation: Ambispora leptoticha + Bradyrhizobium liaoningense; UI un-inoculated, I inoculated, UIS un-inoculated stress, IS inoculated stress, Flowering stage: 1st stress period (35-60 DAS); Pod filling stage: 2nd stress period (85-100 DAS); No significant differences (p ≤ 0.05) observed relative to controls UI & UIS and their respective treatments.
Effects of trophic status, water level, and temperature on shallow lake metabolism and metabolic balance: A standardized pan‐European mesocosm experiment
Creators: name: Scharfenberger, Ulrike
name: Adrian, Rita
name: Jeppesen, Erik
name: Beklioğlu, Meryem
name: Søndergaard, Martin
name: Angeler, David G.
name: Çakıroğlu, Ayşe İdil
name: Drakare, Stina
name: Hejzlar, Josef
name: Mahdy, Aldoushy
Publisher: name: Freie Universität Berlin
Year: 2018
Subjects: subject: metabolism
subject: shallow lakes
subject: trophic state
subject: water level change
subject: temperature threshold
subject: climate change
subject: 500 Naturwissenschaften und Mathematik::590 Tiere (Zoologie)::590 Tiere (Zoologie)

description: Important drivers of gross primary production (GPP) and ecosystem respiration (ER) in lakes are temperature, nutrients, and light availability, which are predicted to be affected by climate change. Little is known about how these three factors jointly influence shallow lakes metabolism and metabolic status as net heterotrophic or autotrophic. We conducted a pan‐European standardized mesocosm experiment covering a temperature gradient from Sweden to Greece to test the differential temperature sensitivity of GPP and ER at two nutrient levels (mesotrophic or eutrophic) crossed with two water levels (1 m and 2 m) to simulate different light regimes. The findings from our experiment were compared with predictions made according the metabolic theory of ecology (MTE). GPP and ER were significantly higher in eutrophic mesocosms than in mesotrophic ones, and in shallow mesocosms compared to deep ones, while nutrient status and depth did not interact. The estimated temperature gains for ER of ~ 0.62 eV were comparable with those predicted by MTE. Temperature sensitivity for GPP was slightly higher than expected ~ 0.54 eV, but when corrected for daylight length, it was more consistent with predictions from MTE ~ 0.31 eV. The threshold temperature for the switch from autotrophy to heterotrophy was lower under mesotrophic (~ 11°C) than eutrophic conditions (~ 20°C). Therefore, despite a lack of significant temperature‐treatment interactions in driving metabolism, the mesocosm's nutrient level proved to be crucial for how much warming a system can tolerate before it switches from net autotrophy to net heterotrophy.
Molecular movie of ultrafast coherent rotational dynamics of OCS
Creators: name: Karamatskos, Evangelos T.
name: Vrakking, Marc J. J.
name: Raabe, Sebastian
name: Mullins, Terry
name: Trabattoni, Andrea
name: Stammer, Philipp
name: Goldsztejn, Gildas
name: Johansen, Rasmus R.
name: Długołecki, Karol
name: Stapelfeldt, Henrik
Publisher: name: Freie Universität Berlin
Year: 2019
Subjects: subject: molecular interactions with photons
subject: atomic interactions with photons
subject: chemical physics
subject: imaging
subject: sensing
subject: optical manipulation
subject: tweezers
subject: 500 Naturwissenschaften und Mathematik::530 Physik::530 Physik

description: Recording molecular movies on ultrafast timescales has been a longstanding goal for unravelling detailed information about molecular dynamics. Here we present the direct experimental recording of very-high-resolution and -fidelity molecular movies over more than one-and-a-half periods of the laser-induced rotational dynamics of carbonylsulfide (OCS) molecules. Utilising the combination of single quantum-state selection and an optimised two-pulse sequence to create a tailored rotational wavepacket, an unprecedented degree of field-free alignment, 〈cos2θ2D〉 = 0.96 (〈cos2θ〉 = 0.94) is achieved, exceeding the theoretical limit for single-pulse alignment. The very rich experimentally observed quantum dynamics is fully recovered by the angular probability distribution obtained from solutions of the time-dependent Schrödinger equation with parameters refined against the experiment. The populations and phases of rotational states in the retrieved time-dependent three-dimensional wavepacket rationalises the observed very high degree of alignment.
Additional file 8 of Ameliorating the drought stress tolerance of a susceptible soybean cultivar, MAUS 2 through dual inoculation with selected rhizobia and AM fungus
Creators: name: Ashwin, Revanna
name: Bagyaraj, Davis Joseph
name: Mohan Raju, Basavaiah
Publisher: name: figshare
Year: 2024
Subjects: subject: Plant Biology
subject: FOS: Biological sciences

description: Additional file 8: Table S4. Summary of cropping and treatment details.
53605: Home [2]
Creators: name: Project Team, First World War Poetry Digital Archive
Publisher: name: University of Oxford
Year: 2024
Subjects: classificationCode: 430304
subject: British history
classificationCode: 430308
subject: European history (excl. British, classical Greek and Roman)
classificationCode: 430316
subject: International history

description: Typescript. The manuscript is listed as '61 Home [2]' in 'Edward Thomas Collected Poems'.
Lessons from LIMK1 enzymology and their impact on inhibitor design
Creators: name: Salah, Eidarus
name: Chatterjee, Deep
name: Beltrami, Alessandra
name: Tumber, Anthony
name: Preuss, Franziska
name: Canning, Peter
name: Chaikuad, Apirat
name: Knaus, Petra
name: Knapp, Stefan
name: Bullock, Alex N.
Publisher: name: Freie Universität Berlin
Year: 2019
Subjects: subject: kinase
subject: LIMK1
subject: reaction mechanism
subject: small-molecule inhibitors
subject: substrate recognition
subject: 500 Naturwissenschaften und Mathematik::540 Chemie::547 Organische Chemie

description: LIM domain kinase 1 (LIMK1) is a key regulator of actin dynamics. It is thereby a potential therapeutic target for the prevention of fragile X syndrome and amyotrophic lateral sclerosis. Herein, we use X-ray crystallography and activity assays to describe how LIMK1 accomplishes substrate specificity, to suggest a unique ‘rock-and-poke’ mechanism of catalysis and to explore the regulation of the kinase by activation loop phosphorylation. Based on these findings, a differential scanning fluorimetry assay and a RapidFire mass spectrometry activity assay were established, leading to the discovery and confirmation of a set of small-molecule LIMK1 inhibitors. Interestingly, several of the inhibitors were inactive towards the closely related isoform LIMK2. Finally, crystal structures of the LIMK1 kinase domain in complex with inhibitors (PF-477736 and staurosporine, respectively) are presented, providing insights into LIMK1 plasticity upon inhibitor binding.
Additional file 1 of Zeaxanthin epoxidase is involved in the carotenoid biosynthesis and light-dependent growth of the marine alga Nannochloropsis oceanica
Creators: name: Liu, Meijing
name: Ding, Wei
name: Pan, Yufang
name: Hu, Hanhua
name: Liu, Jin
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Environmental Sciences not elsewhere classified
subject: Ecology
subject: Biological Sciences not elsewhere classified
subject: Marine Biology
subject: Plant Biology

description: Additional file 1: Figure S1. Proposed carotenoid biosynthetic pathways in N. ocenica. Figure S2. Gene structures of NoZEP1 and NoZEP2. Figure S3. Transmembrane domains predication for NoZEP1 and NoZEP2 by TMHMM (V2.0, http://www.cbs.dtu.dk/services/TMHMM/ ). Figure S4. Predicted domains of NoZEP1 and NoZEP2 via SMART analysis ( http://smart.embl-heidelberg.de/ ). Figure S5. HPLC elution profiles of carotenoids produced in the zeaxanthin-producing E. coli strain without (top) or with NoZEPs (middle and bottom). Figure S6. Subcellular localization predication for NoZEP1 and NoZEP2. Figure S7. Cultures of WT and NoZEP-knockdown lines from 2 days of HL in Fig. 4. Figure S8. Content of TFA and TAG as affected by NoZEP1 or NoZEP2 knockdown in N. oceanica under NL and HL conditions. Figure S9. Content of individual fatty acids of MGDG, DGDG, and SQDG as affected by NoZEP1 or NoZEP2 knockdown in N. oceanica under NL and HL stages. Figure S10. Correlation between chlorophyll a and violaxanthin and between chlorophyll a and total carotenoids in N. oceanica. Figure S11. Correlation between membrane lipids and violaxanthin in N. oceanica. Figure S12. Illustration of vectors used in this study for N. oceanica transformation. Table S1. Primers used in the present study.
sj-docx-1-jbc-10.1177_08839115221098059 – Supplemental material for CMC-based hydrogels loaded with Hypericum perforatum nanoemulsion for potential wound dressing applications
Creators: name: Moradi, Mina
name: Barati, Aboulfazl
name: Moradi, Sara
name: Arjomandzadegan, Mohammad
Publisher: name: SAGE Journals
Year: 2022
Subjects: subject: 91299 Materials Engineering not elsewhere classified
subject: FOS: Materials engineering

description: Supplemental material, sj-docx-1-jbc-10.1177_08839115221098059 for CMC-based hydrogels loaded with Hypericum perforatum nanoemulsion for potential wound dressing applications by Mina Moradi, Aboulfazl Barati, Sara Moradi and Mohammad Arjomandzadegan in Journal of Bioactive and Compatible Polymers
Technical note: drifting versus anchored flux chambers for measuring greenhouse gas emissions from running waters
Creators: name: Lorke, A.
name: Bodmer, Pascal
name: Noss, C.
name: Alshboul, Z.
name: Koschorreck, M.
name: Somlai-Haase, C.
name: Bastviken, D.
name: Flury, S.
name: McGinnis, D. F.
name: Maeck, A.
name: Müller, D.
name: Premke, K.
Publisher: name: Freie Universität Berlin
Year: 2015
Subjects: subject: 500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie

description: Stream networks have recently been discovered to be major but poorly constrained natural greenhouse gas (GHG) sources. A fundamental problem is that several measurement approaches have been used without cross-comparisons. Flux chambers represent a potentially powerful methodological approach if robust and reliable ways to use chambers on running water can be defined. Here we compare the use of anchored and freely drifting chambers on various streams with different flow velocities. The study clearly shows that (1) anchored chambers enhance turbulence under the chambers and thus elevate fluxes, (2) drifting chambers have a very small impact on the water turbulence under the chamber and thus generate more reliable fluxes, (3) the bias of the anchored chambers greatly depends on chamber design and sampling conditions, and (4) there is a promising method to reduce the bias from anchored chambers by using a flexible plastic foil collar to seal the chambers to the water surface, rather than having rigid chamber walls penetrating into the water. Altogether, these results provide novel guidance on how to apply flux chambers in running water, which will have important consequences for measurements to constrain the global GHG balances.
sj-docx-1-bmi-10.1177_11772719221106600 – Supplemental material for Elevated Levels of Pleiotropic Interleukin-6 (IL-6) and Interleukin-10 (IL-10) are Critically Involved With the Severity and Mortality of COVID-19: An Updated Longitudinal Meta-Analysis and Systematic Review on 147 Studies
Creators: name: Jafrin, Sarah
name: Aziz, Md. Abdul
name: Islam, Mohammad Safiqul
Publisher: name: SAGE Journals
Year: 2022
Subjects: Biochemistry

description: Supplemental material, sj-docx-1-bmi-10.1177_11772719221106600 for Elevated Levels of Pleiotropic Interleukin-6 (IL-6) and Interleukin-10 (IL-10) are Critically Involved With the Severity and Mortality of COVID-19: An Updated Longitudinal Meta-Analysis and Systematic Review on 147 Studies by Sarah Jafrin, Md. Abdul Aziz and Mohammad Safiqul Islam in Biomarker Insights
Additional file 5 of Ameliorating the drought stress tolerance of a susceptible soybean cultivar, MAUS 2 through dual inoculation with selected rhizobia and AM fungus
Creators: name: Ashwin, Revanna
name: Bagyaraj, Davis Joseph
name: Mohan Raju, Basavaiah
Publisher: name: figshare
Year: 2024
Subjects: subject: Plant Biology
subject: FOS: Biological sciences

description: Additional file 5: Table S2. Influence of dual inoculation on number of nodules, nodule weight, mycorrhizal spore numbers in the root zone soil and the percent mycorrhizal root colonization in a drought susceptible soybean cultivar, MAUS 2 grown under irrigated and moisture stressed field conditions.
Resilience trinity: safeguarding ecosystem functioning and services across three different time horizons and decision contexts
Creators: name: Weise, Hanna
name: Guilbaud, Camille S. E.
name: Tietjen, Britta
name: Auge, Harald
name: Baessler, Cornelia
name: Bärlund, Ilona
name: Bennett, Elene M.
name: Berger, Uta
name: Bohn, Friedrich
name: Bonn, Aletta
Publisher: name: Freie Universität Berlin
Year: 2020
Subjects: subject: concepts
subject: ecosystems
subject: ecosystem services provisioning
subject: management
subject: resilience
subject: 500 Naturwissenschaften und Mathematik::590 Tiere (Zoologie)::590 Tiere (Zoologie)

description: Ensuring ecosystem resilience is an intuitive approach to safeguard the functioning of ecosystems and hence the future provisioning of ecosystem services (ES). However, resilience is a multi‐faceted concept that is difficult to operationalize. Focusing on resilience mechanisms, such as diversity, network architectures or adaptive capacity, has recently been suggested as means to operationalize resilience. Still, the focus on mechanisms is not specific enough. We suggest a conceptual framework, resilience trinity, to facilitate management based on resilience mechanisms in three distinctive decision contexts and time‐horizons: 1) reactive, when there is an imminent threat to ES resilience and a high pressure to act, 2) adjustive, when the threat is known in general but there is still time to adapt management and 3) provident, when time horizons are very long and the nature of the threats is uncertain, leading to a low willingness to act. Resilience has different interpretations and implications at these different time horizons, which also prevail in different disciplines. Social ecology, ecology and engineering are often implicitly focussing on provident, adjustive or reactive resilience, respectively, but these different notions of resilience and their corresponding social, ecological and economic tradeoffs need to be reconciled. Otherwise, we keep risking unintended consequences of reactive actions, or shying away from provident action because of uncertainties that cannot be reduced. The suggested trinity of time horizons and their decision contexts could help ensuring that longer‐term management actions are not missed while urgent threats to ES are given priority.
Additional file 6 of Ameliorating the drought stress tolerance of a susceptible soybean cultivar, MAUS 2 through dual inoculation with selected rhizobia and AM fungus
Creators: name: Ashwin, Revanna
name: Bagyaraj, Davis Joseph
name: Mohan Raju, Basavaiah
Publisher: name: figshare
Year: 2024
Subjects: subject: Plant Biology
subject: FOS: Biological sciences

description: Additional file 6: Table S3. Influence of dual inoculation on rhizosphere microbial communities and dehydrogenase activity before and after imposing the stress in a drought susceptible soybean cultivar, MAUS 2 grown under irrigated and moisture stressed field conditions
One-year oral toxicity study on a genetically modified maize MON810 variety in Wistar Han RCC rats (EU 7th Framework Programme project GRACE)
Creators: name: Zeljenková, Dagmar
name: Sharbati, Jutta
name: Bohmer, Marc
name: Bohmer, Nils
name: Einspanier, Ralf
name: Aláčová, Radka
name: Ondrejková, Júlia
name: Ambrušová, Katarína
name: Bartušová, Mária
name: Kebis, Anton
Publisher: name: Freie Universität Berlin
Year: 2016
Subjects: subject: Food/Feed Guidance Document of the EFSA Scientific Committee (2011)
subject: genetically modified maize MON810
subject: GRACE
subject: OECD Test Guideline No. 452–Chronic toxicity studies (2009)
subject: rat feeding trial
subject: chronic oral toxicity study
subject: 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit

description: The GRACE (GMO Risk Assessment and Communication of Evidence; www.grace-fp7.eu) project was funded by the European Commission within the 7th Framework Programme. A key objective of GRACE was to conduct 90-day animal feeding trials, animal studies with an extended time frame as well as analytical, in vitro and in silico studies on genetically modified (GM) maize in order to comparatively evaluate their use in GM plant risk assessment. In the present study, the results of a 1-year feeding trial with a GM maize MON810 variety, its near-isogenic non-GM comparator and an additional conventional maize variety are presented. The feeding trials were performed by taking into account the guidance for such studies published by the EFSA Scientific Committee in 2011 and the OECD Test Guideline 452. The results obtained show that the MON810 maize at a level of up to 33 % in the diet did not induce adverse effects in male and female Wistar Han RCC rats after a chronic exposure.
sj-docx-1-tam-10.1177_17588359221105024 – Supplemental material for Network meta-analysis of immune-oncology monotherapy as first-line treatment for advanced non-small-cell lung cancer in patients with PD-L1 expression ⩾50%
Creators: name: Freemantle, Nick
name: Xu, Yingxin
name: Wilson, Florence R.
name: Guyot, Patricia
name: Chen, Chieh-I
name: Keeping, Sam
name: Konidaris, Gerasimos
name: Chan, Keith
name: Kuznik, Andreas
name: Atsou, Kokuvi
name: Glowienka, Emily
name: Pouliot, Jean-Francois
name: Gullo, Giuseppe
name: Rietschel, Petra
Publisher: name: SAGE Journals
Year: 2022
Subjects: subject: 111299 Oncology and Carcinogenesis not elsewhere classified
subject: FOS: Clinical medicine
subject: 111702 Aged Health Care
subject: FOS: Health sciences
subject: 111599 Pharmacology and Pharmaceutical Sciences not elsewhere classified
subject: 110203 Respiratory Diseases

description: Supplemental material, sj-docx-1-tam-10.1177_17588359221105024 for Network meta-analysis of immune-oncology monotherapy as first-line treatment for advanced non-small-cell lung cancer in patients with PD-L1 expression ⩾50% by Nick Freemantle, Yingxin Xu, Florence R. Wilson, Patricia Guyot, Chieh-I Chen, Sam Keeping, Gerasimos Konidaris, Keith Chan, Andreas Kuznik, Kokuvi Atsou, Emily Glowienka, Jean-Francois Pouliot, Giuseppe Gullo and Petra Rietschel in Therapeutic Advances in Medical Oncology
Ninety-day oral toxicity studies on two genetically modified maize MON810 varieties in Wistar Han RCC rats (EU 7th Framework Programme project GRACE)
Creators: name: Zeljenková, Dagmar
name: Sharbati, Jutta
name: Hanisch, Carlos
name: Einspanier, Ralf
name: Ambrušová, Katarína
name: Bartušová, Mária
name: Kebis, Anton
name: Kovrižnych, Jevgenij
name: Krivošíková, Zora
name: Kuricová, Miroslava
Publisher: name: Freie Universität Berlin
Year: 2014
Subjects: subject: Food/Feed Guidance Document of the EFSA Scientific Committee (2011)
subject: genetically modified maize MON810
subject: GRACE
subject: rat feeding trial
subject: subchronic oral toxicity study
subject: 500 Naturwissenschaften und Mathematik::530 Physik::530 Physik

description: The GMO Risk Assessment and Communication of Evidence (GRACE; www.grace-fp7.eu) project is funded by the European Commission within the 7th Framework Programme. A key objective of GRACE is to conduct 90-day animal feeding trials, animal studies with an extended time frame as well as analytical, in vitro and in silico studies on genetically modified (GM) maize in order to comparatively evaluate their use in GM plant risk assessment. In the present study, the results of two 90-day feeding trials with two different GM maize MON810 varieties, their near-isogenic non-GM varieties and four additional conventional maize varieties are presented. The feeding trials were performed by taking into account the guidance for such studies published by the EFSA Scientific Committee in 2011 and the OECD Test Guideline 408. The results obtained show that the MON810 maize at a level of up to 33 % in the diet did not induce adverse effects in male and female Wistar Han RCC rats after subchronic exposure, independently of the two different genetic backgrounds of the event.
Additional file 1 of Utilization of spontaneous breathing trial, objective cough test, and diaphragmatic ultrasound results to predict extubation success: COBRE-US trial
Creators: name: Varón-Vega, Fabio
name: Giraldo-Cadavid, Luis F.
name: Uribe, Ana María
name: Rincón, Adriana
name: Palacios, Jonathan
name: Crevoisier, Stephanie
name: Tuta-Quintero, Eduardo
name: Ordoñez, Lina
name: Boada, Natalia
name: Rincón, Paola
name: Poveda, Marcela
name: Monedero, Pablo
Publisher: name: figshare
Year: 2024
Subjects: subject: Space Science
subject: Biological Sciences not elsewhere classified
subject: Mathematical Sciences not elsewhere classified
subject: Infectious Diseases
subject: FOS: Health sciences

description: Additional file 1. Interaction and collinearity analysis.
sj-docx-1-smo-10.1177_20503121221104436 – Supplemental material for Nurse’s spiritual care perception in Ethiopia: A multicenter cross-sectional study
Creators: name: Seid, Kalid
name: Eneyew, Mekdes
Publisher: name: SAGE Journals
Year: 2022
Subjects: subject: 111708 Health and Community Services
subject: FOS: Health sciences
subject: Cardiology
subject: 110599 Dentistry not elsewhere classified
subject: FOS: Clinical medicine
subject: 110305 Emergency Medicine
subject: 110306 Endocrinology
subject: 110308 Geriatrics and Gerontology
subject: 110904 Neurology and Neuromuscular Diseases
subject: 111099 Nursing not elsewhere classified
subject: 111199 Nutrition and Dietetics not elsewhere classified
subject: 111299 Oncology and Carcinogenesis not elsewhere classified
subject: 110314 Orthopaedics
subject: 110604 Sports Medicine
subject: 110315 Otorhinolaryngology
subject: 111702 Aged Health Care
subject: 111403 Paediatrics
subject: 111599 Pharmacology and Pharmaceutical Sciences not elsewhere classified
subject: 170199 Psychology not elsewhere classified
subject: FOS: Psychology
subject: 110319 Psychiatry (incl. Psychotherapy)
subject: 111799 Public Health and Health Services not elsewhere classified
subject: 110203 Respiratory Diseases
subject: 160807 Sociological Methodology and Research Methods
subject: FOS: Sociology
subject: 110323 Surgery
subject: 70706 Veterinary Medicine
subject: FOS: Veterinary sciences

description: Supplemental material, sj-docx-1-smo-10.1177_20503121221104436 for Nurse’s spiritual care perception in Ethiopia: A multicenter cross-sectional study by Kalid Seid and Mekdes Eneyew in SAGE Open Medicine
Supplemental Material - Motivating Protective Behavior against COVID-19: Fear Versus Hope
Creators: name: Sand, Gregor
name: Bristle, Johanna
Publisher: name: SAGE Journals
Year: 2022
Subjects: subject: 110306 Endocrinology
subject: FOS: Clinical medicine
subject: 110308 Geriatrics and Gerontology
subject: 111799 Public Health and Health Services not elsewhere classified
subject: FOS: Health sciences

description: Supplemental Material for Motivating Protective Behavior against COVID-19: Fear Versus Hope by Gregor Sand, and Johanna Bristle in Journal of Aging and Health
Rapid testing leads to the underestimation of the scrapie prevalence in an affected sheep and goat flock
Creators: name: Reckzeh, Claudia
name: Hoffmann, Christine
name: Fries, Reinhard
name: Buschmann, Anne
name: Buda, Silke
name: Budras, Klaus-Dieter
name: Reckling, Karl-Friedrich
name: Bellmann, Steffi
name: Knobloch, Hartmut
name: Erhardt, Georg
Publisher: name: Freie Universität Berlin
Year: 2007
Subjects: subject: preclinical scrapie
subject: immunohistochemistry
subject: lymphoid tissue
subject: 500 Naturwissenschaften und Mathematik::590 Tiere (Zoologie)::590 Tiere (Zoologie)
subject: 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit

description: To obtain a more detailed understanding of the prevalence of classical scrapie infections in a heavily affected German sheep flock (composed of 603 sheep and 6 goats), we analysed 169 sheep and 6 goats that carried the genotypes susceptible to the disease and that were therefore culled following discovery of the index case. The initial tests were performed using the Biorad TeSeE ELISA and reactive results were verified by official confirmatory methods (OIE-immunoblot and/or immunohistochemistry (IHC)) to demonstrate the deposition of scrapie-associated PrPSc in the brain stem (obex). This approach led to the discovery of 40 additional subclinically scrapie-infected sheep. Furthermore, peripheral lymphatic and nervous tissue samples of the 129 sheep and 6 goats with a negative CNS result were examined by IHC in order to identify any preclinical infections which had not already spread to the central nervous system (CNS). Using this approach we found 13 additional sheep with PrPSc depositions in the gut-associated lymph nodes (GALT) as well as in the enteric nervous system. Moreover, in most of these cases PrPSc was also deposited in the spleen and in the retropharyngeal and superficial cervical lymph nodes. Taken together, these results show a 30.3% infection prevalence in this scrapie-affected flock. Almost 7.4% of the infected animals harboured PrPSc exclusively in the peripheral lymphatic and nervous tissue and were therefore missed by the currently used testing strategy.