Additional file 1 of circFBXO7/miR-96-5p/MTSS1 axis is an important regulator in the Wnt signaling pathway in ovarian cancer
Creators: name: Wu, Mengting
name: Qiu, Qiongzi
name: Zhou, Qing
name: Li, Jia
name: Yang, Juze
name: Zheng, Chengcai
name: Luo, Aoran
name: Li, Xufan
name: Zhang, Honghe
name: Cheng, Xiaodong
name: Lu, Weiguo
name: Liu, Pengyuan
name: Lu, Bingjian
name: Lu, Yan
Publisher: name: figshare
Year: 2022
Subjects: subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Pharmacology
subject: Developmental Biology
subject: Cancer

description: Additional file 1: Table S1. Demographic and clinical information of ovarian cancer patients used in this study. Table S2. Quality control metrics of rRNA-depleted RNA-seq libraries in this study. Table S3. Sequences of primers and siRNAs used in this study. Table S4. Quality control metrics of ployA-enriched RNA-seq libraries in this study. Table S5. Information of antibodies used in the study. Figure S1. Genomic information of circFBXO7. (A) circBase annotation of circFBXO7 (ID: hsa_circ_0001222). (B) Sequence of full-length circFBXO7 from Sanger sequencing. Figure S2. circFBXO7 exerts tumor suppressive effects in ovarian cancer cells. (A) qRT-PCR analysis of circFBXO7 and linear-FBXO7 expression in SKOV3 and OV90 cells transfected with circFBXO7 siRNA and negative control siRNA. (B) Growth curve of SKOV3 and OV90 cells transfected with circFBXO7 siRNA and negative control siRNA, as assessed by CCK-8 assays. (C) Migration ability of SKOV3 and OV90 cells transfected with circFBXO7 siRNA and negative control siRNAs, as assessed by transwell assays. (D) Invasion ability of SKOV3 and OV90 cells transfected with circFBXO7 siRNA and negative control siRNAs, as assessed by transwell assays. Figure S3. MTSS1 inhibits ovarian cancer cell proliferation and migration. (A) qRT-PCR analysis of MTSS1 expression in A2780 and MDAH2774 cells transfected with MTSS1 overexpression and mock plasmids. (B) Western blot analysis of the expression of MTSS1 in A2780 and MDAH2774 cells transfected with MTSS1 overexpression and mock plasmids. (C) Growth curve of A2780 and MDAH2774 transfected with MTSS1 overexpression and mock plasmids, as assessed by CCK-8 assays. (D) Proliferation of A2780 and MDAH2774 cells transfected with MTSS1 overexpression and mock plasmids, as determined by colony formation assay. (E) Migration ability of A2780 and MDAH2774 cells transfected with MTSS1 overexpression and mock plasmids. (F) Western blot analysis of the expression of VIM and snail in A2780 and MDAH2774 cells transfected with MTSS1 siRNA and negative control siRNA. Student’s two-sided t tests were used. *** P &lt; 0.001, ** P &lt; 0.01, * P &lt; 0.05. Figure S4. Heat map of differentially expressed genes between circFBXO7-overexpressing and control ovarian cancer cells. Figure S5. circFBXO7/MTSS1 is involved in the regulation of Wnt/β-catenin signaling. (A) Western blot analysis of the expression of wnt/β-catenin and p-GSK3β in A2780 and MDAH2774 cells transfected with MTSS1 overexpression and mock plasmids. (B) Western blot analysis of the expression of β-catenin and p-GSK3β in A2780 and MDAH2774 cells transfected with circFBXO7 siRNA or negative controls. (C) Western blot analysis of the expression of β-catenin and p-GSK3β in MTSS1 overexpression A2780 and MDAH2774 cells transfected with LiCl, NaCl, or mock plasmids. (D) Representative images of IF micrographs of the subcellular localization and expression of β-catenin (green) in MTSS1 overexpression MDAH2774 cells transfected with LiCl. Nuclei were counterstained with DAPI (blue). Student’s two-sided t tests were used. *** P &lt; 0.001, ** P &lt; 0.01, * P &lt; 0.05. Figure S6. circFBXO7 and FBXO7 expression in low- and high-grade ovarian tumors. (A) CircFBXO7 expression in our FFPE cohort. (B) FBXO7 expression in the TCGA cohort.