title: ÐÐµÑÐµÐºÑÐ¸Ñ RET-ÑÑÐ°Ð½ÑÐ»Ð¾ÐºÐ°ÑÐ¸Ð¹ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ ÐÐ¢-ÐÐ¦Ð Ð² Ð¾Ð¿ÑÑ Ð¾Ð»ÑÑ Ð»ÑÐ³ÐºÐ¸Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Detection of RET translocations by RT-PCR in lung tumors
Creators: name: ÐÑÐ¸Ð²Ð¾ÑÐµÐµÐ²Ð°, ÐÐ»ÐµÐ½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÑÐ°Ðº Ð»ÐµÐ³ÐºÐ¾Ð³Ð¾
ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ½ÑÐµ ÑÐ¸ÑÐ¾Ð·Ð¸Ð½ÐºÐ¸Ð½Ð°Ð·Ñ
ÑÑÐ°Ð½ÑÐ»Ð¾ÐºÐ°ÑÐ¸Ñ
Ð½ÐµÑÐ±Ð°Ð»Ð°Ð½ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð°Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ
Ð²Ð°ÑÐ¸Ð°Ð½Ñ-ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐµÑÐºÐ°Ñ ÐÐ¦Ð
lung cancer
receptor tyrosine kinases
translocation
unbalansed expression
variant-independent detection PCR assays

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description: The given work is devoted to the study of major and rare RET-translocations and optimization of testing lung tumor samples for their detection. Reverse transcription polymerase chain reaction (RT-PCR) was chosen as a method of analysis because of its availability, the possibility to use nucleic acid samples from paraffin blocks that do not require special storage conditions, and high sensitivity. The assay methodology includes both assessment for unbalanced expression of RET gene and detection of a specific translocation variant using a separate, specific, PCR assay.In the work, 7475 samples were analyzed, and the rearrangement was detected in 131 of them (mutation incidence was 1.1%). A total of 9 mutation variants were identified, of which 3 were rare. The unbalanced expression test correlated with the presence of a chimeric transcript in 93.8% of cases. According to the results of statistical processing, an association with female gender was determined and no association with age was detected. This analysis is relevant and clinically demanded due to the use of selective medicines (tyrosine kinase inhibitors) targeting tumor suppression of receptor tyrosine kinase translocation in oncology practice.