title: ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÑÑÐ»Ð»ÐµÑÐµÐ½Ð¾Ð²ÑÑ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´Ð½ÑÑ Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾ÑÐ¾Ð² ÑÐ¸Ð±Ð¾Ð½ÑÐºÐ»ÐµÐ°Ð·Ð½Ð¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð¾Ð±ÑÐ°ÑÐ½Ð¾Ð¹ ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ°Ð·Ñ ÐÐÐ§-1
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Study of the effectiveness of fullerene derivatives as inhibitors of the ribonuclease activity of HIV-1 reverse transcriptase
Creators: name: ÐÐ°Ð»ÐµÐµÐ²Ð°, ÐÐ¸Ð»ÑÑÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2025
Subjects: Ð²Ð¸Ñ-1
Ð¾Ð±ÑÐ°ÑÐ½Ð°Ñ ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ°Ð·Ð°
ÑÐ¸Ð±Ð¾Ð½ÑÐºÐ»ÐµÐ°Ð·Ð½Ð°Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ
ÑÑÐ»Ð»ÐµÑÐµÐ½Ñ
hiv-1
reverse transcriptase
ribonuclease activity
fullerenes

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description: This work is devoted to the analysis of the ability of four fullerene derivatives with different side radicals to inhibit the ribonuclease activity of HIV-1 reverse transcriptase. Tasks that were solved in the course of the study: â conduct an analysis of current literature on the research topic; â select fullerene derivatives for research: three samples have charged acid radicals: residues of carboxylic, sulfo- and phosphoric acids; and the fourth is an uncharged ester group; â study the activity of fullerene derivatives with different structures of side radicals in the system using gel electrophoresis under denaturing conditions and determine IC50 (half-maximal inhibition concentration); â optimize the conditions for analyzing RNase H activity in plates (method based on fluorescence detection): select the structure of the substrate, as well as select the concentrations of the enzyme and substrate; determine IC50 for the same fullerene derivatives in a spot system; â compare inhibition data obtained by two different methods. The object of the study is fullerene derivatives with different structures of radicals. Three substances have charged acid radicals: residues of carboxylic, sulfo- and phosphoric acids, and the fourth derivative has an uncharged ester group. The main analyzed parameter was half-maximal inhibitory concentration. The study found that each of the four fullerene derivatives could inhibit the ribonuclease activity of HIV-1 reverse transcriptase. It was also established that the IC50 values btained by the two methods were correlated. The fullerene derivative with phosphoric acid residues in its radicals was the most active, while the fullerene derivative with carboxylic acid residues in its radicals was the least active. When writing the literature review, the modern databases in biotechnology, bioanalytical chemistry and microbiological analysis (Elibrary, Scopus, Google Scholar, Elsevier, Google Scholar) were used. Statistical processing of experimental data was carried out using the software package: Microsoft Excel, Quantity OneTM 4.6.6, GraphPad Prism 7.