title: ÐÑÐ¾Ð±ÐµÐ½Ð½Ð¾ÑÑÐ¸ ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¿Ð¾Ñ Ð½Ð¸Ð·Ð¸Ð½Ð¾Ð¼ Ð² Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ð°Ñ Ð¸Ð· Ð³Ð»Ð¸ÑÐµÑÐ¾ÑÐ¾ÑÑÐ¾Ð»Ð¸Ð¿Ð¸Ð´Ð¾Ð²
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Features of pore formation by nisin in membranes from glycerophospholipids
Creators: name: Ð§ÐµÑÐ½ÑÑÐ¾Ð²Ð°, ÐÐ°ÑÐ½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÐÐ¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ñ
ÐÐ¸Ð¾ÑÐµÑ Ð½Ð¾Ð»Ð¾Ð³Ð¸Ñ
Ð½Ð¸Ð·Ð¸Ð½
Ð»Ð¸Ð¿Ð¸Ð´Ð½ÑÐµ Ð±Ð¸ÑÐ»Ð¾Ð¸
Ð»Ð¸Ð¿Ð¾ÑÐ¾Ð¼Ñ
Ð³Ð»Ð¸ÑÐµÑÐ¾ÑÐ¾ÑÑÐ¾Ð»Ð¸Ð¿Ð¸Ð´
Ð¼Ð°Ð»ÑÐµ Ð¼Ð¾Ð»ÐµÐºÑÐ»Ñ
nisin
lipid bilayers
liposomes
glycerophospholipid
small molecules

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description: The aim of the work: the investigation of the molecular mechanisms of lantibiotic nisin action on the model lipid membranes and the detection of the potential regulator of the pore-forming activity of nisin using small molecules of various chemical structures. Electrophysiological and fluorimetric methods were used to measure the bilayer boundary and dipole potential, respectively; the measurement of the leakage of a fluorescent marker from lipid vesicles was used to measure the membrane permeability in the presence of nisin. The single channels formed by nisin and its multichannel activity were studied. Phloretin, RH 421, tetracaine, 3-benzyl-5-phenyl-3H-1,2,3,4-dithiadiazole-2-oxide, and N'-(3,5-difluorophenyl)- benzenecarbothiohydrazide were used as potential regulators of the pore-forming activity of nisin. It was found that nisin forms the transmembrane pores in the membranes, including the phosphoglycerol and cardiolipin, which may indicate the specificity of its action in the bilayers. Phloretin and N'-(3,5-difluorophenyl)-benzenecarbothiohydrazide were found to potentiate the membrane activity of nisin in cardiolipin-containing membranes. It was found that the influence of small molecules on the packing density of lipids in the membrane does not affect the membrane activity of the lantibiotic and is due to a change in the membrane boundary potential during the adsorption of small molecules.