Additional file 8: Figure S5. of The genome of the yellow potato cyst nematode, Globodera rostochiensis, reveals insights into the basis of parasitism and virulence
Creators: name: Akker, Sebastian Eves-Van Den
name: Laetsch, Dominik
name: Thorpe, Peter
name: Lilley, Catherine
name: Danchin, Etienne
name: Rocha, Martine Da
name: Rancurel, Corinne
name: Holroyd, Nancy
name: Cotton, James
name: Szitenberg, Amir
name: Grenier, Eric
name: Montarry, Josselin
name: Mimee, Benjamin
name: Marc-Olivier Duceppe
name: Boyes, Ian
name: Marvin, Jessica
name: Jones, Laura
name: Hazijah Yusup
name: JoĂŤl Lafond-Lapalme
name: Esquibet, Magali
name: Sabeh, Michael
name: Rott, Michael
name: Overmars, Hein
name: Finkers-Tomczak, Anna
name: Smant, Geert
name: Koutsovoulos, Georgios
name: Blok, Vivian
name: Mantelin, Sophie
name: Cock, Peter
name: Phillips, Wendy
name: Henrissat, Bernard
name: Urwin, Peter
name: Blaxter, Mark
name: Jones, John
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Genetics
subject: Molecular Biology
subject: Evolutionary Biology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: FOS: Earth and related environmental sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: 60506 Virology
subject: Computational Biology

description: Phylogenetic analysis of Worm-specific Argonauts (WAGOs). A. A total of 604 nematode and non-nematode argonaute proteins drawn as an unrooted phylogram. It is assumed that each subtree (Bâ  F) is effectively rooted by the other subtrees; however, the extreme divergence between these proteins yields low support for some subtrees. Bâ  F Subtrees containing G. rostochiensis WAGOs (GrWAGOs). Branch widths of subtrees are drawn proportional to branch support. Coloured boxes indicate membership of taxa to phylogenetic groups (nematode Clade I, Clade III, Clade IV and Clade V, non-nematode taxa) and coloured stars indicate clades composed entirely of Heteroderidae-WAGOs (Globodera spp., Meloidogyne spp., Heterodera glycines). Orange dots indicate GrWAGOs in differential expression super-clusters 19, 20 or 21. GrWAGOs are placed within Nematode WAGO-subclades ALG1/ALG2 (1), RDE1/ERGO1/PRG1/2 (2), WAGO1/2/4/5 (7), CSR/WAGO-III/WAGO-IV (8) and NRDE/WAGO-10/11 (5), sensu Buck and Blaxter, 2013 [30]. As expected, no GrWAGOs were observed in WAGO-subclades SAGO2/PPW, WAGO-III/-V and ALG3/ALG4. Subclades NRDE/WAGO-10/11, WAGO1/2/4/5 and CSR/WAGO-III/WAGO-IV show increased numbers of paralogous expansion of these gene families within Clade IV in general and Heteroderidae in particular. B All Globodera spp. and Meloidogyne spp. exhibit one ALG1/ALG2 orthologue each, which form a clade. C The RDE1/ERGO1/PRG1/2 subtree contains another Heteroderidae-specific clade; however, one M. incognita sequence is sister to a subclade of Clade III taxa. This is surprising since the Clade III parasites A. suum and B. pahangi are thought to have lost the piRNA pathway [30] and may be an artefact. D The NRDE/WAGO-10/WAGO-11 subtree shows an expansion of paralogous Heteroderidae-WAGOs, with three GrWAGOs expressed at 14 dpi. E The WAGO1/2/4/5 subtree depicts two Heterodera-specific expansions, of which the larger subclade contains four GrWAGOs expressed at 14 dpi. F The CSR/WAGO-III/WAGO-IV subtree contains another two Heteroderidae-specific expansion, of which one also includes a sequence from the Clade V nematode Dictyocaulus viviparus (ochre star). (PDF 64 kb)