title: Ð¤Ð»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð°Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ Ð¿Ð¾Ð»Ð½Ð¾Ð³Ð¾ Ð²Ð½ÑÑÑÐµÐ½Ð½ÐµÐ³Ð¾ Ð¾ÑÑÐ°Ð¶ÐµÐ½Ð¸Ñ Ð´Ð»Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¼Ð°ÐºÑÐ¾Ð¼Ð¾Ð»ÐµÐºÑÐ»
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Total internal reflection fluorescence microscopy for the study of macromolecules
Creators: name: Ð¤Ð°Ð½ Ð®Ð¹Ñ ÑÐ½
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: Ð¾Ð´Ð¸Ð½Ð¾ÑÐ½ÑÐµ Ð¼Ð¾Ð»ÐµÐºÑÐ»Ñ
Ð¼Ð°ÐºÑÐ¾Ð¼Ð¾Ð»ÐµÐºÑÐ»Ñ
ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ñ
ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð°Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ Ð¿Ð¾Ð»Ð½Ð¾Ð³Ð¾ Ð²Ð½ÑÑÑÐµÐ½Ð½ÐµÐ³Ð¾ Ð¾ÑÑÐ°Ð¶ÐµÐ½Ð¸Ñ
ÑÐ²Ð»ÐµÐ½Ð¸Ðµ Ð¿Ð¾Ð»Ð¾Ð³Ð¾ Ð²Ð½ÑÑÑÐµÐ½Ð½ÐµÐ³Ð¾ Ð¾ÑÑÐ°Ð¶ÐµÐ½Ð¸Ñ
single molecules
macromolecules
fluorescence
total internal reflection fluorescence microscopy
hollow internal reflection phenomenon

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ ÑÐ¸Ð·Ð¸ÑÐµÑÐºÐ¸Ñ Ð¾ÑÐ½Ð¾Ð² Ð¼ÐµÑÐ¾Ð´Ð° ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸ Ð¿Ð¾Ð»Ð½Ð¾Ð³Ð¾ Ð²Ð½ÑÑÑÐµÐ½Ð½ÐµÐ³Ð¾ Ð¾ÑÑÐ°Ð¶ÐµÐ½Ð¸Ñ Ð¸ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐµ Ð½Ð° ÐµÐ³Ð¾ Ð¾ÑÐ½Ð¾Ð²Ðµ ÑÑÐµÐ¼Ñ ÑÑÑÐ°Ð½Ð¾Ð²ÐºÐ¸ Ð´Ð»Ñ Ð´ÐµÑÐµÐºÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¾Ð´Ð¸Ð½Ð¾ÑÐ½ÑÑ Ð¼Ð¾Ð»ÐµÐºÑÐ» ÐºÑÐ°ÑÐ¸ÑÐµÐ»Ñ ÑÐ¾Ð´Ð°Ð¼Ð¸Ð½Ð° 6 G. Ð ÑÐ¾Ð´Ðµ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¸ ÑÐµÑÐµÐ½Ñ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð·Ð°Ð´Ð°ÑÐ¸: Ð¾Ð±Ð¾ÑÐ½Ð¾Ð²Ð°Ð½Ð° Ð¿ÑÐ¸Ð¼ÐµÐ½Ð¸Ð¼Ð¾ÑÑÑ Ð¸ Ð°ÐºÑÑÐ°Ð»ÑÐ½Ð¾ÑÑÑ Ð²ÑÐ±ÑÐ°Ð½Ð½Ð¾Ð³Ð¾ Ð¼ÐµÑÐ¾Ð´Ð°, ÑÐ°ÑÑÐ¼Ð¾ÑÑÐµÐ½Ñ ÑÐ¸Ð·Ð¸ÑÐµÑÐºÐ¸Ðµ Ð¾ÑÐ½Ð¾Ð²Ñ Ð¼ÐµÑÐ¾Ð´Ð°, ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð° ÑÑÐµÐ¼Ð° Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð°, ÑÐ°ÑÑÑÐ¸ÑÐ°Ð½Ñ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÑ ÑÑÐµÐ¼Ñ Ð¸ Ð¿ÑÐ¸Ð²ÐµÐ´ÐµÐ½Ð¾ Ð¾Ð±Ð¾ÑÐ½Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð²ÑÐ±ÑÐ°Ð½Ð½ÑÑ ÑÐ»ÐµÐ¼ÐµÐ½ÑÐ¾Ð². ÐÐ¾Ð²Ð¸Ð·Ð½Ð° Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ Ð·Ð°ÐºÐ»ÑÑÐ°ÐµÑÑÑ Ð² ÑÐ¾Ð¼, ÑÑÐ¾ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð½Ð°Ñ ÑÑÐµÐ¼Ð° ÑÑÑÐ°Ð½Ð¾Ð²ÐºÐ¸ Ð¸Ð¼ÐµÐµÑ Ð¼Ð°Ð»ÑÐµ Ð³Ð°Ð±Ð°ÑÐ¸ÑÑ, ÑÐ¾ÑÑÐ¾Ð¸Ñ Ð¸Ð· Ð½ÐµÐ´Ð¾ÑÐ¾Ð³Ð¾ÑÑÐ¾ÑÑÐ¸Ð¹ ÑÐ»ÐµÐ¼ÐµÐ½ÑÐ¾Ð²: Ð¿Ð¾Ð»ÑÐ¿ÑÐ¾Ð²Ð¾Ð´Ð½Ð¸ÐºÐ¾Ð²Ð¾Ð³Ð¾ Ð»Ð°Ð·ÐµÑÐ° Ð²Ð¼ÐµÑÑÐ¾ ÑÐ²ÐµÑÐ´Ð¾ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ Ð½ÐµÐ¾Ð´Ð¸Ð¼Ð¾Ð²Ð¾Ð³Ð¾ Ð»Ð°Ð·ÐµÑÐ°, ÐºÐ¾ÑÐ¾ÑÑÐ¹ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·ÑÐµÑÑÑ Ð² ÐºÐ¾Ð¼Ð¼ÐµÑÑÐµÑÐºÐ¸Ð¹ Ð¿ÑÐ¸Ð±Ð¾ÑÐ¾Ð², Ð° ÑÐ°ÐºÐ¶Ðµ Ð°Ð»ÑÑÐµÑÐ½Ð°ÑÐ¸Ð²Ð½Ð°Ñ Ð³ÐµÐ¾Ð¼ÐµÑÑÐ¸Ñ Ð²Ð¾Ð·Ð±ÑÐ¶Ð´ÐµÐ½Ð¸Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸, Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÐºÐ¾ÑÐ¾ÑÐ¾Ð¹ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾ ÑÐ¼ÐµÐ½ÑÑÐ¸ÑÑ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ ÑÐ¾Ð½Ð¾Ð²Ð¾Ð¹ Ð·Ð°ÑÐ²ÐµÑÐºÐ¸ Ð¸ ÑÐ²ÐµÐ»Ð¸ÑÐ¸ÑÑ ÑÐ¾Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ðµ ÑÐ¸Ð³Ð½Ð°Ð»/ÑÑÐ¼.
description: This work is devoted to the study of the physical foundations of the method of fluorescence microscopy of total internal reflection and the development on its basis of an experimental microscope scheme for the detection of single molecules of the dye rhodamine 6 G. In the course of the work the following problems were solved: the applicability and relevance of the selected method were considered, the physical foundations of the method, the optical scheme of the microscope was developed, the parameters of the scheme were calculated, and the substantiation of the selected elements was given. The novelty of this work lies in the fact that the developed setup scheme has small dimensions, consists of inexpensive elements: a semiconductor laser instead of a solid-state neodymium laser, which is used in commercial devices, as well as an alternative geometry of fluorescence excitation, with which it is possible to reduce influence of background light and increase the signal-to-noise ratio.