Additional file 1 of Sodium levels and grazing pressure shape natural communities of the intracellular pathogen Legionella
Creators: name: Bergman, Oded
name: Be’eri-Shlevin, Yaron
name: Ninio, Shira
Publisher: name: figshare
Year: 2023
Subjects: subject: Ecology
subject: FOS: Biological sciences

description: Additional file 1: Supplementary Methods. Supplementary Figure 1. Map of sampling stations. Supplementary Figure 2. Principal-component analysis (PCA) of the various physiochemical characteristics measured at each sampling site. Supplementary Figure 3. Loading scores of Prinicipal-Component Analysis (PCA) of selected physiochemical characteristics measured at each sampling site, representing the four spring clusters. Supplementary Figure 4. Hierarchical K-means clustering of Legionella spp. abundance between different samples. Supplementary Figure 5. Rarefaction curves for Next generation Sequencing (NGS). Supplementary Figure 6. Analysis of batch effect between the Next Generation Sequencing (NGS) centers. Supplementary Figure 7. Legionella spp. levels do not correlate between water and biofilm samples. Supplementary Figure 8. Alpha and beta diversity of Legionella spp. microbial population in water vs. biofilm samples. Supplementary Figure 9. Venn diagram of Legionella spp. in water and biofilm samples. Supplementary Figure 10. Alpha diversity of Legionella spp. across spring clusters. Supplementary Figure 11. Alpha diversity of potential Legionella hosts. Supplementary Figure 12. Phylogenetic analysis of Legionella amplicon sequence variants (ASVs) in water samples. Supplementary Figure 13. Cooccurrence of Legionella ASVs with protozoan-hosts in Tabgha and Fuliya spring clusters. Supplementary Table 1. Physiochemical characteristics of selected springs stations surrounding lake Kinneret. Supplementary Table 2. Ranges of physicochemical characteristics for the studied springs sites surrounding lake Kinneret. Supplementary Table 3. Ranges of physicochemical characteristics representing springs clusters surrounding lake Kinneret. Supplementary Table 4. Loading scores of environmental variables included in the initial PCA analysis. Supplementary Table 5. Correlations between the physicochemical parameters comprising the initial PCA. Supplementary Table 6. Loading scores of environmental variables included in the final PCA analysis. Supplementary Table 7. Correlations between the selected physicochemical parameters included in the final PCA. Supplementary Table 8. K-means clustering summery table for qPCR heatmap (Fig. 2C). Supplementary Table 9. Kruskal-Wallis and post hoc via Wilcox test for Legionella spp. qPCR levels, between the different spring clusters and collection dates. Supplementary Table 10. Feature and sequence counts for all primer sets used in the study. Supplementary Table 11. Kruskal-Wallis test results of 16S NGS alpha diversity between the two sequencing centers. Supplementary Table 12. PERMANOVA, PERMDISP and pairwise post hoc results of 16S NGS beta between the two sequencing centers, collection years and water vs. biofilm. Supplementary Table 13. Kruskal-Wallis and post hoc via Wilcox tests of 16S NGS alpha diversity between the different spring clusters and collection dates. Supplementary Table 14. PERMANOVA and PERMDISP results of 16S NGS beta diversity between the different spring clusters. Supplementary Table 15. PERMANOVA and PERMDISP post hoc pairwise comparison results of 16S NGS beta diversity between the different spring clusters. Supplementary Table 16. ADONIS test results of 16S NGS beta diversity with selected environmental variables. Supplementary Table 17. Kruskal-Wallis test results of 18S NGS alpha diversity between the two sequencing centers. Supplementary Table 18. PERMANOVA and PERMDISP results of 18S NGS beta diversity between the two sequencing centers and collection years. Supplementary Table 19. Kruskal-Wallis and post hoc via Wilcox tests of 18S NGS alpha diversity between the different spring clusters and collection dates. Supplementary Table 20. PERMANOVA and PERMDISP results of 18S NGS beta diversity between the different spring clusters. Supplementary Table 21. PERMANOVA and PERMDISP post hoc pairwise comparison results of 18S NGS beta diversity between the different spring clusters.