cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
An updated checklist of marine and brackish water Amphipoda (Crustacea: Peracarida) of the southern Bay of Biscay (NE Atlantic)
Creators: name: BACHELET, Guy
name: DAUVIN, Jean-Claude
name: SORBE, Jean-Claude
Publisher: name: Station Biologique de Roscoff (SBR)
Year: 2003

description: An updated list of the marine and brackish water amphipods recorded from the southern Bay of Biscay, i.e. from
 46°N and 9°W to the Atlantic coastlines of Spain and France, is given. This checklist, based on literature review and
 unpublished observations, includes recent systematics revision and provides distributional and ecological data for each
 species. A total of 319 species (14 Caprellidea, 284 Gammaridea, 21 Hyperiidea) has been recorded; this number is higher
 than in the English Channel which is a continental shelf sea, while the number of Gammaridea species is similar to that of
 the Mediterranean French waters. Nineteen species (15 in the bathyal zone, 2 on the continental shelf, and 2 in the intertidal
 zone) appear "endemic" to the Bay of Biscay. Bathymetric distribution shows that the highest amphipod diversity is found
 in the 0-10 m depth zone (148 species). This study also emphasizes the importance of bathyal amphipods (131 species),
 especially on the lower continental slope (500-2000 m depth), related to the presence of Cap Ferret and Capbreton canyons.
Finite-Elemente-Methode am 1D-Beispiel singulärer Randwertprobleme
Creators: name: Homberger, Stefanie
Publisher: name: Zenodo
Year: 2023
Subjects: FEM
Finite-Elemente-Methode
1D
singuläre Randwertprobleme

description: Die Finite-Elemente-Methode (FEM) ist eine mathematische Technik zur Lösung von Differentialgleichungen, die in vielen ingenieurwissenschaftlichen Anwendungen Verwendung findet. Diese Arbeit konzentriert sich auf die Anwendung der FEM im eindimensionalen Fall auf singuläre Randwertprobleme. Dabei werden die Probleme numerisch mithilfe eigens entwickelter Programme in Matlab gelöst.
description: Download: https://www.amazon.de/dp/B0C5W4S1T5
Testicular Expression of Adora3i2 in Adora3 Knockout Mice Reveals a Role of Mouse A3Ri2 and Human A3Ri3 Adenosine Receptors in Sperm
Creators: name: Unadkat, Jashvant D.
name: Burnett, Lindsey A.
name: Tilley, Stephen L.
name: Blais, Edik M.
name: Babcock, Donner F.
name: Hille, Bertil
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2010

description: Adenosine is a candidate modulator of sperm motility in the female reproductive tract that increases sperm flagellar beat frequency in vitro. Past work suggested that this acceleration may involve equilibrative (ENT) and concentrative (CNT) nucleoside transporters. Here we show that Slc29a1 (ENT-1) is the predominant nucleoside transporter expressed in the mouse testis. Unexpectedly, the beat of Slc29a1-null sperm still accelerates in response to 2-chloro-2′-deoxyadenosine (Cl-dAdo). Moreover, in wild-type sperm neither blockade of CNTs by removal of external Na+, nor inhibition of ENTs with nitrobenzylthioionosine, prevents acceleration of the sperm beat by Cl-dAdo. In contrast, pertussis toxin produces strong blockade, indicating involvement of a Gαi/o-coupled adenosine receptor. Although agonists selective for adenosine receptors A1R, A2aR, and A2bR are ineffective, A3R-selective agonists Cl-IB-MECA and IB-MECA do accelerate the beat. Consistent with this pharmacological profile, the predominant Adora transcripts in the testis are products of the nested Adora3i1 and Adora3i2 genes. Surprisingly, Cl-IB-MECA and Cl-dAdo still accelerate the beat of Adora3i1-null sperm indicating that the remaining Adora3i2 transcript produces an A3R that functions in sperm. When cloned Adora3i2 is heterologously expressed in tsA-201 cells, Cl-dAdo decreases forskolin-evoked accumulation of cAMP, indicating that Adora3i2 specifies a functional A3Ri2 adenosine receptor that couples through Gαi. Database mining reveals that mouse Adora3i2 is expressed primarily in testis, almost exclusively in spermatids. Expression of the orthologous ADORA3i3 transcript also is most prominent in human testis; presumably producing an A3Ri3 receptor that is functional in sperm and that may be a target for development of male-directed contraceptives.
(3999) Mary Ann Banks Taig
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Two new species of deep-water caprellids (Crustacea: Amphipoda) from northeastern Brazil
Creators: name: GUERRA-GARCÍA, José Manuel
Publisher: name: Station Biologique de Roscoff (SBR)
Year: 2003

description: Two new caprellid species, Liropus nelsonae sp. nov. and Parvipalpus colemani sp. nov. are described and
 illustrated based on specimens of deep-sea samples collected from northeastern Brazil. Liropus nelsonae is the only species
 in the genus that has pereopod 5 three-articulate. Parvipalpus colemani can be distinguished from the remaining species of
 Parvipalpus mainly by the presence of abdominal appendages and a mandibular palp with 7-10 apical setae. Liropus
 nelsonae and Parvipalpus colemani represent the southernmost record of the genera Liropus and Parvipalpus respectively.
 A key to the world species of the genera Liropus and Parvipalpus and an up-to-date list of the deep-sea caprellidea are
 included.
LES ACTIVITÉS RYTHMIQUES COMPORTEMENTALES DE LA COLONIE DE VERETILLUM CYNOMORIUM (CNIDAIRE PENNATULIDAE)
Creators: name: BUISSON, Bernard
Publisher: name: Station Biologique de Roscoff (SBR)
Year: 1971

description: The Veretillum, possessing a rachis with polyps and a stalk, is either contracted and wormlike or turgid with expanded polyps. A long expansion takes place between both aspects. Then, the first aspect is to be found again after a general contraction. The rachis and the stalk show movements. Some of which enable the implantation of the foot.
The presence of rhythmical behavioural activities of middle and high frequenciesareprecised.
Among the middle frequency rhythms an endogenous one with external correlate (light) is discriminated. This Pennatulid expands during the evening and contracts on the next day. Under constant conditions the regular succession continues chiefly with anchored animals.
The high frequency rhythms observed during the same time correspond to peristaltic waves and pulsations. These give successive sequencies of various frequencies.
The anchorage only takes place when the Veretillum is contracted and appears if peristaltic waves and pulsations show a sufficient frequency.
The different fragments issued from the Veretillum sectionning show the localization of these activities. Pacemakers are regionally recognized. So expansion pacemakers and anchorage pacemakers are respectively found in the rachis and in the proximal part of the stalk. The pacemakers which fonction one after the other give local or general sequences. In the two cases transmission systems are necessary. State changes are also suggested.
These data are related to those provided elsewhere by ultrastructural histology (Buisson, 1970 a) and by the study of spontaneous electrical activities and of motor reactions during behavioural rhythms (Buisson, 1970 b).
Additional file 3 of Mental health resilience in offspring of depressed parents: a systematic literature review protocol
Creators: name: Padaigaitė, Eglė
name: Maruyama, Jessica Mayumi
name: Hammerton, Gemma
name: Rice, Frances
name: Collishaw, Stephan
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Biotechnology
subject: Cancer
subject: Science Policy
subject: 111714 Mental Health
subject: FOS: Health sciences

description: Additional file 3. Full search strategy for each database
The Cystic Fibrosis Transmembrane Conductance Regulator Impedes Proteolytic Stimulation of the Epithelial Na + Channel
Creators: name: Dang, Hong
name: Boucher, Richard C.
name: Garcia-Caballero, Agustin
name: Gentzsch, Martina
name: Dang, Yan
name: Suchindran, Hamsa
name: Stutts, M. Jackson
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2010

description: Cystic fibrosis (CF) is caused by mutations in the CF transmembrane conductance regulator (CFTR) that prevent its proper folding and trafficking to the apical membrane of epithelial cells. Absence of cAMP-mediated Cl− secretion in CF airways causes poorly hydrated airway surfaces in CF patients, and this condition is exacerbated by excessive Na+ absorption. The mechanistic link between missing CFTR and increased Na+ absorption in airway epithelia has remained elusive, although substantial evidence implicates hyperactivity of the epithelial Na+ channel (ENaC). ENaC is known to be activated by selective endoproteolysis of the extracellular domains of its α- and γ-subunits, and it was recently reported that ENaC and CFTR physically associate in mammalian cells. We confirmed this interaction in oocytes by co-immunoprecipitation and found that ENaC associated with wild-type CFTR was protected from proteolytic cleavage and stimulation of open probability. In contrast, ΔF508 CFTR, the most common mutant protein in CF patients, failed to protect ENaC from proteolytic cleavage and stimulation. In normal airway epithelial cells, ENaC was contained in the anti-CFTR immunoprecipitate. In CF airway epithelial cultures, the proportion of full-length to total α-ENaC protein signal was consistently reduced compared with normal cultures. Our results identify limiting proteolytic cleavage of ENaC as a mechanism by which CFTR down-regulates Na+ absorption.
Decentralized Sequential Composite Hypothesis Test Based on One-Bit Communication
Creators: name: Li, Shang
name: Li, Xiaoou
name: Wang, Xiaodong
name: Liu, Jingchen
Publisher: name: arXiv
Year: 2015
Subjects: subject: Applications (stat.AP)
subject: Information Theory (cs.IT)
subject: FOS: Computer and information sciences

description: This paper considers the sequential composite hypothesis test with multiple sensors. The sensors observe random samples in parallel and communicate with a fusion center, who makes the global decision based on the sensor inputs. On one hand, in the centralized scenario, where local samples are precisely transmitted to the fusion center, the generalized sequential likelihood ratio test (GSPRT) is shown to be asymptotically optimal in terms of the expected sample size as error rates tend to zero. On the other hand, for systems with limited power and bandwidth resources, decentralized solutions that only send a summary of local samples (we particularly focus on a one-bit communication protocol) to the fusion center is of great importance. To this end, we first consider a decentralized scheme where sensors send their one-bit quantized statistics every fixed period of time to the fusion center. We show that such a uniform sampling and quantization scheme is strictly suboptimal and its suboptimality can be quantified by the KL divergence of the distributions of the quantized statistics under both hypotheses. We then propose a decentralized GSPRT based on level-triggered sampling. That is, each sensor runs its own GSPRT repeatedly and reports its local decision to the fusion center asynchronously. We show that this scheme is asymptotically optimal as the local thresholds and global thresholds grow large at different rates. Lastly, two particular models and their associated applications are studied to compare the centralized and decentralized approaches. Numerical results are provided to demonstrate that the proposed level-triggered sampling based decentralized scheme aligns closely with the centralized scheme with substantially lower communication overhead, and significantly outperforms the uniform sampling and quantization based decentralized scheme.
description: 39 pages
The Hippo Tumor Pathway Promotes TAZ Degradation by Phosphorylating a Phosphodegron and Recruiting the SCF β-TrCP E3 Ligase
Creators: name: Lei, Qun Ying
name: Zhao, Shimin
name: Guan, Kun Liang
name: Huang, Wei
name: Zha, Zheng Yu
name: Li, Tingting
name: Liu, Chen Ying
name: Lim, Chun Jye
name: Hong, Wanjin
name: Chan, Siew Wee
name: Xiong, Yue
name: Zhang, Heng
name: Zhao, Di
name: Zhou, Xin
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2010

description: The TAZ transcription co-activator promotes cell proliferation and epithelial-mesenchymal transition. TAZ is inhibited by the Hippo tumor suppressor pathway, which promotes TAZ cytoplasmic localization by phosphorylation. We report here that TAZ protein stability is controlled by a phosphodegron recognized by the F-box protein β-TrCP and ubiquitylated by the SCF/CRL1β-TrCP E3 ligase. The interaction between TAZ and β-TrCP is regulated by the Hippo pathway. Phosphorylation of a phosphodegron in TAZ by LATS primes it for further phosphorylation by CK1ϵ and subsequent binding by β-TrCP. Therefore, the Hippo pathway negatively regulates TAZ function by both limiting its nuclear accumulation and promoting its degradation. The phosphodegron-mediated TAZ degradation plays an important role in negatively regulating TAZ biological functions.
Additional file 1 of Dental caries and periodontitis and the risk of myopia in young adults: CHIEF oral health study
Creators: name: Tsai, Kun-Zhe
name: Liu, Pang-Yen
name: Lin, Yen-Po
name: Pao, Shu-I.
name: Tai, Ming-Cheng
name: Chen, Jiann-Torng
name: Lin, Gen-Min
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Physiology
subject: FOS: Biological sciences
subject: Sociology
subject: FOS: Sociology
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: Science Policy
subject: 111714 Mental Health
subject: FOS: Health sciences

description: Additional file 1: Table 1. Association of Stage I and Stage II/III Periodontitis With Myopia
The Insulin-like Growth Factor-1 Binding Protein Acid-labile Subunit Alters Mesenchymal Stromal Cell Fate
Creators: name: Sun, Hui
name: Courtland, Hayden Williams
name: Kawashima, Yuki
name: Yakar, Shoshana
name: Wu, Yinjgie
name: Clemmons, David
name: Elis, Sebastien
name: Fritton, J. Christopher
name: Rosen, Clifford J.
name: Mejia, Wilson
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2010

description: Age-related osteoporosis is accompanied by an increase in marrow adiposity and a reduction in serum insulin-like growth factor-1 (IGF-1) and the binding proteins that stabilize IGF-1. To determine the relationship between these proteins and bone marrow adiposity, we evaluated the adipogenic potential of marrow-derived mesenchymal stromal cells (MSCs) from mice with decreased serum IGF-1 due to knockdown of IGF-1 production by the liver or knock-out of the binding proteins. We employed 10–16-week-old, liver-specific IGF-1-deficient, IGFBP-3 knock-out (BP3KO) and acid-labile subunit knock-out (ALSKO) mice. We found that expression of the late adipocyte differentiation marker peroxisome proliferator-activated receptor γ was increased in marrow isolated from ALSKO mice. When induced with adipogenic media, MSC cultures from ALSKO mice revealed a significantly greater number of differentiated adipocytes compared with controls. MSCs from ALSKO mice also exhibited decreased alkaline-phosphatase positive colony size in cultures that were stimulated with osteoblast differentiation media. These osteoblast-like cells from ALSKO mice failed to induce osteoclastogenesis of control cells in co-culture assays, indicating that impairment of IGF-1 complex formation with ALS in bone marrow alters cell fate, leading to increased adipogenesis.
title: Fool me thrice: a case report of a twice-missed fracture of the lateral process of the talus (snowboarder’s fracture)
Creators: name: Harvey, James
Publisher: name: The Royal Australian and New Zealand College of Radiologists
Year: 2017
Subjects: subject: Athletic injuries
subject: Diagnostic procedure
subject: Plain radiographic studies
subject: CT
subject: Trauma
subject: Musculoskeletal bone
subject: Anatomy

description: Learning objectives Background Imaging findings OR Procedure details Conclusion Personal information References
description: Learning objectives: Fractures of the lateral process of the talus (LPT) are frequently misdiagnosed - the mechanism, typical presentation and management of this fracture is presented. Appreciate that these fractures are often comminuted and displaced and may cause...
High temperature conductance of a two-dimensional superlattice controlled by spin-orbit interaction
Creators: name: Foldi, Peter
name: Szaszko-Bogar, Viktor
name: Peeters, F. M.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Mesoscale and Nanoscale Physics (cond-mat.mes-hall)
subject: Quantum Physics (quant-ph)
subject: FOS: Physical sciences

description: Rashba-type spin-orbit interaction (SOI) controlled band structure of a two-dimensional superlattice allows for the modulation of the conductance of finite size devices by changing the strength of the SOI. We consider rectangular arrays and find that the temperature dependence of the conductance disappears for high temperatures, but the strength of the SOI still affects the conductance at these temperatures. The modulation effect can be seen even in the presence of strong dephasing, which can be important in case of practical applications.
description: 6 pages 7 figures
title: The sensitivity and role of ultrasonography in patients with clinically suspected appendicitis and the predictors of a high-yield study.
Creators: name: Denby, Edward
Publisher: name: The Royal Australian and New Zealand College of Radiologists
Year: 2017
Subjects: subject: Colon
subject: Gastrointestinal tract
subject: Abdomen
subject: Ultrasound
subject: Diagnostic procedure
subject: Observer performance
subject: Inflammation
subject: Acute

description: Purpose Methods and materials Results Conclusion Personal information References
description: Purpose: Ultrasound for suspected appendicitis has a variable diagnostic accuracy with sensitivities ranging from 76% to 90% and speciﬁcities between 83% and 100% (1) . Anecdotally, rates of equivocal results at the Gold Coast University Hospital (GCUH)...
(3994) Mary Ann Banks Taig
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
LARVAL TRANSPORT AND RECRUITMENT IN THE INTERTIDAL POPULATION OF POLYDORA-CILIATA (ANNELIDA, POLYCHAETA) AT BOULOGNE-SUR-MER
Creators: name: Lagadeuc, Y.
name: Brylinski, J. M.
Publisher: name: Station Biologique de Roscoff (SBR)
Year: 1987

Abstract
title: Image guided percutaneous pleural biopsy diagnostic yield and accuracy for benign and malignant pleural lesions
Creators: name: Saffar, Bann
Publisher: name: The Royal Australian and New Zealand College of Radiologists
Year: 2017
Subjects: subject: Thorax
subject: Interventional non-vascular
subject: CT
subject: Ultrasound
subject: Fluoroscopy
subject: Biopsy
subject: Cancer

description: Purpose Methods and materials Results Conclusion Personal information References
description: Purpose: Pleural biopsy can be performed medicaly via pleuroscopy, surgicaly via video-assistend thoracoscopic surgery (VATS) and open surgical thoracotomy or by image guidance. Image-guided pleural biopsy is less invasive procedure, less expensive and...
The Mechanism of Microcin C Resistance Provided by the MccF Peptidase
Creators: name: Tikhonov, Anton
name: Van Aerschot, Arthur
name: Reader, John S.
name: Severinov, Konstantin
name: Serebryakova, Marina
name: Govorun, Vadim M.
name: Kazakov, Teymur
name: Vondenhoff, Gaston
name: Semenova, Ekaterina
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2010

description: The heptapeptide-nucleotide microcin C (McC) is a potent inhibitor of enteric bacteria growth. Inside a sensitive cell, McC is processed by aminopeptidases, which release a nonhydrolyzable aspartyl-adenylate, a strong inhibitor of aspartyl-tRNA synthetase. The mccABCDE operon is sufficient for McC production and resistance of the producing cell to McC. An additional gene, mccF, which is adjacent to but not part of the mccABCDE operon, also provides resistance to exogenous McC. MccF is similar to Escherichia coli LdcA, an l,d-carboxypeptidase whose substrate is monomeric murotetrapeptide l-Ala-d-Glu-meso-A2pm-d-Ala or its UDP-activated murein precursor. The mechanism by which MccF provides McC resistance remained unknown. Here, we show that MccF detoxifies both intact and processed McC by cleaving an amide bond between the C-terminal aspartate and the nucleotide moiety. MccF also cleaves the same bond in nonhydrolyzable aminoacyl sulfamoyl adenosines containing aspartyl, glutamyl, and, to a lesser extent, seryl aminoacyl moieties but is ineffective against other aminoacyl adenylates.
title: Radiology and pathology of thymoma relevant to clinical management: series of 16 cases.
Creators: name: Premarathna, Medagodage DN
Publisher: name: The Royal Australian and New Zealand College of Radiologists
Year: 2017
Subjects: subject: Thorax
subject: CT
subject: Technology assessment
subject: Observer performance
subject: Staging
subject: Pathology
subject: Neoplasia

description: Learning objectives Background Imaging findings OR Procedure details Conclusion Personal information References
description: Learning objectives: To assess and highlight the features of pathology and radiology of 16 cases of thymoma with relevant to clinical...
Contractions from $osp(1|32) \oplus osp(1|32)$ to the M-theory superalgebra extended by additional fermionic generators
Creators: name: Fernandez, J. J.
name: Izquierdo, J. M.
name: del Olmo, M. A.
Publisher: name: arXiv
Year: 2015
Subjects: subject: High Energy Physics - Theory (hep-th)
subject: Mathematical Physics (math-ph)
subject: FOS: Physical sciences

description: We study here the generalized Weimar-Woods contractions of the superalgebra $osp(1|32) \oplus osp(1|32)$ in order to obtain a suitable algebra that could describe the gauge group of $D=11$ supergravity. The contracted superalgebras are assumed to be given in terms of fermionic extensions of the M-theory superalgebra. We show that the only superalgebra of this type obtained by contraction is the only one for which the three-form of $D=11$ supergravity cannot be trivialized. Therefore, $D=11$ supergravity cannot be connected in this way with a contraction of $osp(1|32) \oplus osp(1|32)$.
(3996) Mary Reid
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
First episode of vibriosis in wild specimens of Pagellus bogaraveo (Brünnich, 1768) in the Mediterranean Sea
Creators: name: MANCUSO, Monique
name: GENOVESE, Maria
name: GUERRERA, Maria Cristina
name: CASELLA, Gianluca
name: GENOVESE, Lucrezia
name: PICCOLO, Giovani
name: MARICCHIOLO, Giulia
Publisher: name: Station Biologique de Roscoff (SBR)
Year: 2015

description: The blackspot seabream Pagellus bogaraveo (Brünnich, 1768) is a carnivorous fish species and is a promising candidate for the diversification of European aquaculture thanks to the high commercial value and its adaptability to intensive farming. In this paper the authors reported the first case of vibriosis in Pagellus bogaraveo wild specimens. 36 wild blackspot sea bream caught in Sicily, were transported and placed into the tanks of the Experimental Aquaculture Plant of the Institute for Coastal Marine Environment (IAMC-CNR) of Messina. Two days after the capture fish presented external hemorrhages and skin ulcers reaching 69% of cumulative mortality. Some specimens were sacrificed, previous euthanasia, for the histological, bacteriological and molecular assays. Results showed that the disease was a Vibriosis caused by Vibrio anguillarum and the histopathological examination revealed severe damages of the liver, kidney and spleen.
Tipin-Replication Protein A Interaction Mediates Chk1 Phosphorylation by ATR in Response to Genotoxic Stress
Creators: name: Kaufmann, William K.
name: Sancar, Aziz
name: Abraham, Robert T.
name: Smith-Roe, Stephanie L.
name: Akan, Zafer
name: Cordeiro-Stone, Marila
name: Yilmaz, Seçil
name: Grillo, Mary
name: Kemp, Michael G.
name: Ünsal-Kaçmaz, Keziban
name: Kang, Tae Hong
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2010

description: Mammalian Timeless is a multifunctional protein that performs essential roles in the circadian clock, chromosome cohesion, DNA replication fork protection, and DNA replication/DNA damage checkpoint pathways. The human Timeless exists in a tight complex with a smaller protein called Tipin (Timeless-interacting protein). Here we investigated the mechanism by which the Timeless-Tipin complex functions as a mediator in the ATR-Chk1 DNA damage checkpoint pathway. We find that the Timeless-Tipin complex specifically mediates Chk1 phosphorylation by ATR in response to DNA damage and replication stress through interaction of Tipin with the 34-kDa subunit of replication protein A (RPA). The Tipin-RPA interaction stabilizes Timeless-Tipin and Tipin-Claspin complexes on RPA-coated ssDNA and in doing so promotes Claspin-mediated phosphorylation of Chk1 by ATR. Our results therefore indicate that RPA-covered ssDNA not only supports recruitment and activation of ATR but also, through Tipin and Claspin, it plays an important role in the action of ATR on its critical downstream target Chk1.
Transcriptional Synergy between Melanoma Antigen Gene Protein-A11 (MAGE-11) and p300 in Androgen Receptor Signaling
Creators: name: Askew, Emily B.
name: Wilson, Elizabeth M.
name: Bai, Suxia
name: Blackwelder, Amanda J.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2010

description: Androgen receptor (AR)-mediated gene regulation involves interactions with coregulatory proteins that include the melanoma antigen gene protein-A11 (MAGE-11). To understand the functional significance of sequence similarity between MAGE-11 and the adenovirus early protein E1A, we determined whether MAGE-11 contributes to AR transcriptional activity through an interaction with p300, a potent and ubiquitous transcriptional regulator. Here, we report that MAGE-11 interacts with the NH2-terminal region of p300 through the MAGE-11 MXXIF motif 185MXXIF189, with transcriptional activity depending on the MAGE-11 F-box and MAPK phosphorylation. The MAGE-11- and p300-dependent increase in AR transactivation required the NH2-terminal regions of AR and p300, p300 acetyltransferase activity, and the AR FXXLF motif 23FQNLF27 interaction with MAGE-11. MAGE-11 linked AR to p300 and the p160 coactivator, transcriptional intermediary protein 2 (TIF2). The p300 NH2-terminal FXXLF motif 33FGSLF37 was required for transcriptional activation by TIF2. Increased expression of p300 decreased the ubiquitinylation of MAGE-11 and transiently increased endogenous MAGE-11 levels. Autoacetylation of p300 and decreased acetylation of TIF2 were evident in the MAGE-11, p300, and TIF2 complex. The studies suggest that MAGE-11 links NH2-terminal domains of AR and p300 to promote transcriptional synergy through a cadre of FXXLF-related interacting motifs.
Tryptophanyl-tRNA Synthetase Urzyme: A MODEL TO RECAPITULATE MOLECULAR EVOLUTION AND INVESTIGATE INTRAMOLECULAR COMPLEMENTATION
Creators: name: Carter Jr., Charles W.
name: Weinreb, Violetta
name: Hu, Hao
name: Kuhlman, Brian
name: Pham, Yen
name: Butterfoss, Glenn L.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2010

description: We substantiate our preliminary description of the class I tryptophanyl-tRNA synthetase minimal catalytic domain with details of its construction, structure, and steady-state kinetic parameters. Generating that active fragment involved deleting 65% of the contemporary enzyme, including the anticodon-binding domain and connecting peptide 1, CP1, a 74-residue internal segment from within the Rossmann fold. We used protein design (Rosetta), rather than phylogenetic sequence alignments, to identify mutations to compensate for the severe loss of modularity, thus restoring stability, as evidenced by renaturation described previously and by 70-ns molecular dynamics simulations. Sufficient solubility to enable biochemical studies was achieved by expressing the redesigned Urzyme as a maltose-binding protein fusion. Michaelis-Menten kinetic parameters from amino acid activation assays showed that, compared with the native full-length enzyme, TrpRS Urzyme binds ATP with similar affinity. This suggests that neither of the two deleted structural modules has a strong influence on ground-state ATP binding. However, tryptophan has 103 lower affinity, and the Urzyme has comparably reduced specificity relative to the related amino acid, tyrosine. Molecular dynamics simulations revealed how CP1 may contribute significantly to cognate amino acid specificity. As class Ia editing domains are nested within the CP1, this finding suggests that this module enhanced amino acid specificity continuously, throughout their evolution. We call this type of reconstructed protein catalyst an Urzyme (Ur prefix indicates original, primitive, or earliest). It establishes a model for recapitulating very early steps in molecular evolution in which fitness may have been enhanced by accumulating entire modules, rather than by discrete amino acid sequence changes.
Unraveling the Specificity of Heparanase Utilizing Synthetic Substrates
Creators: name: Liu, Jian
name: Peterson, Sherket B.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2010

description: Heparanase is a promising anticancer target because of its involvement in cancer invasion and metastasis. Heparanase cleaves heparan sulfate (HS), a sulfated polysaccharide, and activates a series of HS-mediated cell proliferation and angiogenesis processes. Understanding the substrate specificity of heparanase will aid the discovery of heparanase inhibitors. Here, we sought to determine the specificity of heparanase using synthetic polysaccharide substrates. The substrates were prepared using purified HS biosynthetic enzymes. Using these substrates, we were able to dissect the structural moieties required for heparanase. Our data suggest that heparanase cleaves the linkage between a GlcA unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. In addition, heparanase cleaves the linkage of a GlcA unit and N-sulfo glucosamine unit with a 2-O-sulfated GlcA residue, not a 2-O-sulfated IdoA residue, in proximity. We also discovered that the polysaccharide with repeating disaccharide units of IdoA2S-GlcNS inhibits the activity of heparanase. Our findings advance the understanding of the substrate specificity of heparanase and identify a lead compound for developing polysaccharide-based heparanase inhibitors.
Financial risk : charter school-specific risk factors and idiosyncratic risk
Creators: name: Hinojosa, Luis Paul
Publisher: name: The University of Texas at Austin
Year: 2023
Subjects: Charter school
Charter school specific risk factor
Credit enhancement
Credit risk
Debt guarantee
Education revenue bond
Financial risk
Idiosyncratic risk
Investment risk premium
Nonsystematic risk
Risk management

description: The literature lacked resources or frameworks for identifying and managing charter school-specific risk (CSSR) factors that could affect the investors’ risk perceptions in K-12 education. This study aimed to evaluate how CSSR factors and idiosyncratic risk predict the perceived credit risk of an open enrollment public charter school and the resulting investment risk premiums required by potential investors. The correlational research design included all Texas open enrollment charter schools with municipal education revenue bonds. The analysis included two phases. Phase 1 involved performing simple linear regression modeling to understand the unique contribution of predictor variables to explain the investment risk premium. The significant predictor variables were identified in Phase 1. Phase 2 involved applying multiple linear regression modeling to determine the optimal set of predictor variables influencing investment risk premium. Phase 1 yielded a finding that participation in the Texas Bond Guarantee Program explained 66.7% of the variability in the investment risk premium in each Texas municipal education revenue bond transaction since 2019. On a standalone basis, the CSSR Factor 11 of unrestricted net assets explained 45.5% of the variability in the investment risk premium. In Phase 2, the multivariate linear regression modeling indicated that the predictive power of CSSR Factors 2 through 12 diminished significantly when the TPSF guaranteed a municipal revenue education bond transaction. The addition of a debt guarantee introduced a factor, or possibly factors, outside of the idiosyncratic CSSR factors associated with each municipal education revenue bond issuance. Due to the addition of the TPSF guarantee, the explanatory or predictive power of the CSSR factors diminished from 60.8% to 19.5%. Additionally, a combination of CSSR factors was determined to explain 60.8% of the variability in the investment risk premium. This empirically based understanding of financial risk based on how CSSR factors impact risk perception of open enrollment public charter schools could benefit the efficiency of free public schools. In addition, the state of Texas could benefit from individual public charter schools proactively and deliberately understanding and managing their CSSR factors and idiosyncratic risk. Further recommendations appear in Chapter 5.
Using Engineered 2- O -Sulfotransferase to Determine the Activity of Heparan Sulfate C 5 -epimerase and Its Mutants
Creators: name: Bethea, Heather N.
name: Li, Kai
name: Liu, Jian
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2010

description: Heparan sulfate (HS) is involved in essential physiological and pathophysiological functions. HS is a highly sulfated polysaccharide consisting of glucuronic acid (or iduronic acid) linked to glucosamine carrying various sulfo groups. Biosynthesis of HS involves sulfotransferases and an epimerase. The HS C5-epimerase converts glucuronic acid to iduronic acid. The method for determining the activity has been cumbersome due to the use of a site-specifically 3H-labeled polysaccharide substrate. Here, we report a two-enzyme coupling assay to determine the activity of C5-epimerase. HS 2-O-sulfotransferase (2OST) transfers the sulfo group to the 2-OH-position of glucuronic or iduronic acid. Unlike the wild type protein, 2-O-sulfotransferase mutant (2OST Y94I) transfers sulfate to the iduronic acid but not to the glucuronic acid. Thus, 2OST Y94I cannot sulfate N-sulfated heparosan, a polysaccharide containing glucuronic acid. Incubating N-sulfated heparosan with C5-epimerase converts some of the glucuronic acid to iduronic acid, thus becoming a substrate for 2OST Y94I. The susceptibility of the C5-epimerase-treated N-sulfated heparosan to 2OST Y94I modification directly correlates to the amount of the activity of C5-epimerase, proving that this two-enzyme coupling system can be used to assay for C5-epimerase. The method was further used to determine the activities of various C5-epimerase mutants. Our approach will significantly reduce the complexity for assaying the activity of C5-epimerase and facilitate the structural and functional analysis of C5-epimerase.
(3995) James Menelaws
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Additional file 1 of Effect of Omega-3 fatty acids supplementation on serum level of C-reactive protein in patients with COVID-19: a systematic review and meta-analysis of randomized controlled trials
Creators: name: Taha, Amira Mohamed
name: Shaarawy, Ahmad Shehata
name: Omar, Mohamed Mosad
name: Abouelmagd, Khaled
name: Shalma, Noran Magdy
name: Alhashemi, Mais
name: Ahmed, Hala Mahmoud
name: Allam, Ahmed Hafez
name: Abd-ElGawad, Mohamed
Publisher: name: figshare
Year: 2022
Subjects: subject: Biochemistry
subject: Medicine
subject: Neuroscience
subject: Biotechnology
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Science Policy

description: Additional file 1: PRISMA Checklist.
Interferences in quantum eraser reveal geometric phases in modular and weak values
Creators: name: Cormann, Mirko
name: Remy, Mathilde
name: Kolaric, Branko
name: Caudano, Yves
Publisher: name: arXiv
Year: 2015
Subjects: subject: Quantum Physics (quant-ph)
subject: FOS: Physical sciences

description: In this letter, we present a new procedure to determine completely the complex modular values of arbitrary observables of pre- and post-selected ensembles, which works experimentally for all measurement strengths and all post-selected states. This procedure allows us to discuss the physics of modular and weak values in interferometric experiments involving a qubit meter. We determine both the modulus and the argument of the modular value for any measurement strength in a single step, by controlling simultaneously the visibility and the phase in a quantum eraser interference experiment. Modular and weak values are closely related. Using entangled qubits for the probed and meter systems, we show that the phase of the modular and weak values has a topological origin. This phase is completely defined by the intrinsic physical properties of the probed system and its time evolution. The physical significance of this phase can thus be used to evaluate the quantumness of weak values.
Southeastward expansion of the blue crab Callinectes sapidus (Rathbun, 1896) in the Black Sea
Creators: name: AK, Orhan
name: HAŞİMOĞLU, Atilla
name: BAYRAM, Kaan
Publisher: name: Station Biologique de Roscoff (SBR)
Year: 2015

description: There are already thirteen previously published reported records of the blue crab Callinectes sapidus (Portunidae) from the Black Sea. However, this is the first record coming from the southern easternmost part of the Black Sea (Trabzon, Turkey). The carapace length, width and fresh weight of this female were 19.1 cm, 9.0 cm and 545 g, respectively.
Acireductone Dioxygenase 1 (ARD1) Is an Effector of the Heterotrimeric G Protein  Subunit in Arabidopsis
Creators: name: Jiang, Kun
name: Deshpande, Aditi
name: Friedman, Erin J.
name: Pochapsky, Thomas C.
name: Wang, Helen X.
name: Jones, Alan M.
name: Hicks, Stephanie N.
name: Harden, T. Kendall
name: Perovic, Iva
name: Temple, Brenda R.S.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: Heterotrimeric G protein complexes are conserved from plants to mammals, but the complexity of each system varies. Arabidopsis thaliana contains one Gα, one Gβ (AGB1), and at least three Gγ subunits, allowing it to form three versions of the heterotrimer. This plant model is ideal for genetic studies because mammalian systems contain hundreds of unique heterotrimers. The activation of these complexes promotes interactions between both the Gα subunit and the Gβγ dimer with enzymes and scaffolds to propagate signaling to the cytoplasm. However, although effectors of Gα and Gβ are known in mammals, no Gβ effectors were previously known in plants. Toward identifying AGB1 effectors, we genetically screened for dominant mutations that suppress Gβ-null mutant (agb1-2) phenotypes. We found that overexpression of acireductone dioxygenase 1 (ARD1) suppresses the 2-day-old etiolated phenotype of agb1-2. ARD1 is homologous to prokaryotic and eukaryotic ARD proteins; one function of ARDs is to operate in the methionine salvage pathway. We show here that ARD1 is an active metalloenzyme, and AGB1 and ARD1 both control embryonic hypocotyl length by modulating cell division; they also may contribute to the production of ethylene, a product of the methionine salvage pathway. ARD1 physically interacts with AGB1, and ARD enzymatic activity is stimulated by AGB1 in vitro. The binding interface on AGB1 was deduced using a comparative evolutionary approach and tested using recombinant AGB1 mutants. A possible mechanism for AGB1 activation of ARD1 activity was tested using directed mutations in a loop near the substrate-binding site.
A PROPOS D'UNE ÉVENTUELLE PARENTÉ DE DEUX CHLOROPHYCÉES MARINES : ACROCHAETE REPENS ET BOLBOCOLEON PILIFERUM (CHAETOPHORACÉES-ULOTRICHALES)
Creators: name: KERMARREC, André
Publisher: name: Station Biologique de Roscoff (SBR)
Year: 1970

Abstract
Reproductive cycle and gonad development of the commercial clam Donax trunculus from the Bay of Tunis (Northern Tunisia, South Mediterranean coast): effects of environmental variability
Creators: name: BOUSSOUFA, Dhouha
name: GHAZALI, Nabila
name: RABEH, Imen
name: SOUDANI, Nejla
name: NAVARRO, Juan Carlos
name: EL CAFSI, Mhamed
Publisher: name: Station Biologique de Roscoff (SBR)
Year: 2015

description: In Tunisia the commercial exploitation of Donax trunculus is limited to the unregulated catches which threaten the biological sustainability of this species. Knowledge of the gametogenic development of a species is a prerequisite for proper management and sustainable exploitation of this fishery. The reproductive cycle of D. trunculus from the Bay of Tunis was carried out between November 2006 and January 2008, using the dry weight changes, the histological examination of the gonad and the measurement of oocyte size. Results showed that, sex ratio were in favor of males without any hermaphroditism. The gametogenesis activity of both sexes, started with decreasing seawater temperature during late autumn and the main spawning event took place from June to October when temperatures rose. During this time, clams loose, gradually, their weights and condition and maturity indices reached their lowest values immediately after spawning period. The mean oocyte diameter varied significantly between 12.1 ± 9.8 µm in early gametogenesis and 45.9 ±13.6 µm during maturity. Considering these data, a closed season from May to July coinciding with the most intensive gamete release should be considered for the preservation and the management of the natural stock of D. trunculus from the Bay of Tunis.
title: Protect your neck – What every radiologist should know about the clearance of cervical spinal column injury
Creators: name: Gajera, Jay
Publisher: name: The Royal Australian and New Zealand College of Radiologists
Year: 2017
Subjects: subject: Trauma
subject: Emergency
subject: Musculoskeletal spine
subject: CT
subject: Computer Applications-Detection, diagnosis
subject: Decision analysis
subject: Trauma

description: Learning objectives Background Imaging findings OR Procedure details Conclusion Personal information References
description: Learning objectives: Normal 0 false false false EN-AU X-NONE X-NONE Demonstrate an understanding of the clinical decision tools (Canadian C-spine clearance rules and NEXUS criteria) which identify patients that require further imaging. Be familiar with the major studies...
Anti-heparan Sulfate Peptides That Block Herpes Simplex Virus Infection in Vivo
Creators: name: Valyi-Nagy, Tibor
name: Shukla, Deepak
name: Tiwari, Vaibhav
name: Liu, Jian
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: Heparan sulfate (HS) and its highly modified form, 3-O-sulfated heparan sulfate (3-OS HS), contribute strongly to herpes simplex virus type-1 (HSV-1) infection in vitro. Here we report results from a random M13-phage display library screening to isolate 12-mer peptides that bind specifically to HS, 3-OS HS, and block HSV-1 entry. The screening identified representative candidates from two-different groups of anti-HS peptides with high positive charge densities. Group 1, represented by G1 peptide (LRSRTKIIRIRH), belongs to a class with alternating charges (XRXRXKXXRXRX), and group 2, represented by G2 peptide (MPRRRRIRRRQK), shows repetitive charges (XXRRRRXRRRXK). Viral entry and glycoprotein D binding assays together with fluorescent microscopy data indicated that both G1 and G2 were potent in blocking HSV-1 entry into primary cultures of human corneal fibroblasts and CHO-K1 cells transiently expressing different glycoprotein D receptors. Interestingly, G2 peptide isolated against 3-OS HS displayed wider ability to inhibit entry of clinically relevant strains of HSV-1 and some divergent members of herpesvirus family including cytomegalovirus and human herpesvirus-8. To identify functional residues within G1 and G2, we performed point mutations and alanine-scanning mutagenesis. Several arginine and a lysine residues were needed for anti-HSV-1 activity, suggesting the importance of the positively charged residues in virus-cell binding and virus-induced membrane fusion. In vivo administration of G1 or G2 peptide as a prophylactic eye drop completely blocked HSV-1 spread in the mouse cornea as evident by immunohistochemistry. This result also highlights an in vivo significance of HS and 3-OS HS during ocular herpes infection.