Different gene expression profiles in normo- and dyslipidemic men after fish oil supplementation: Results from a randomized controlled trial
Creators: name: Schmidt, Simone
name: Stahl, Frank
name: Mutz, Kai-Oliver
name: Scheper, Thomas
name: Hahn, Andreas
name: Schuchardt, Jan Philipp
Publisher: name: London : BioMed Central Ltd.
Year: 2012
Subjects: subject: Cardiovascular disease
subject: Dyslipidemia
subject: Gene regulation
subject: Genome microarrays
subject: Hypertriglyceridemia
subject: Lipid metabolism
subject: Omega-3 fatty acids
subject: Omega-3 index
subject: Pathway analysis
subject: cholesterol
subject: docosahexaenoic acid
subject: fish oil
subject: high density lipoprotein
subject: icosapentaenoic acid
subject: low density lipoprotein
subject: membrane lipid
subject: omega 3 fatty acid
subject: triacylglycerol
subject: adult
subject: antiinflammatory activity
subject: article
subject: cardiovascular disease
subject: clinical article
subject: controlled study
subject: diet supplementation
subject: double blind procedure
subject: down regulation
subject: dyslipidemia
subject: erythrocyte membrane
subject: gene expression profiling
subject: gene expression regulation
subject: human
subject: immune system
subject: immunomodulation
subject: lipid blood level
subject: lipid composition
subject: lipid metabolism
subject: microarray analysis
subject: nucleotide sequence
subject: randomized controlled trial
subject: Adult
subject: Corn Oil
subject: Double-Blind Method
subject: Dyslipidemias
subject: Erythrocyte Membrane
subject: Fatty Acids
subject: Fatty Acids, Omega-3
subject: Fish Oils
subject: Humans
subject: Immune System
subject: Lipid Metabolism
subject: Lipids
subject: Male
subject: Middle Aged
subject: Oligonucleotide Array Sequence Analysis
subject: RNA
subject: Transcriptome
subject: Zea mays
subject: Dewey Decimal Classification::500 | Naturwissenschaften
subject: Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie

description: Background: Epidemiological studies have suggested the benefits of omega-3 polyunsaturated fatty acids (n-3 PUFAs) on cardiovascular health, but only limited data are available describing n-3 PUFA regulated pathways in humans. The aim of this study was to investigate the effects of n-3 PUFA administration on whole genome expression profiles in the blood of normo- and dyslipidemic subjects. Methods. Differentially expressed genes were detected after four hours, one week and twelve weeks of supplementation with either fish oil (FO) or corn oil in normo- and dyslipidemic men using whole genome microarrays. Results: Independent of the oil, a significantly higher number of genes was regulated in dyslipidemic subjects compared to normolipidemic subjects. Pathway analyses discovered metabolisms dominantly affected by FO after twelve weeks of supplementation, including the lipid metabolism, immune system and cardiovascular diseases. Several pro-inflammatory genes, in particular, were down-regulated in dyslipidemic subjects, indicating the immune-modulatory and anti-inflammatory capability of FO and its bioactive FAs, eicosapentaenoic acid and docosahexaenoic acid. Conclusions: This is the first study showing significant differences in gene expression profiles between normo- and dyslipidemic men after FO supplementation. Further studies need to clarify the exact role of n-3 PUFAs in pathways and metabolisms which were identified as being regulated after FO supplementation in this study. Trial registration. ClinicalTrials.gov (ID: NCT01089231).