title: Ð¡Ð¾Ð·Ð´Ð°Ð½Ð¸Ðµ Ð¼ÐµÑÐ¾Ð´Ð° Ð°Ð½Ð°Ð»Ð¸Ð·Ð° ÑÐ¸ÑÐºÑÐ»Ð¸ÑÑÑÑÐ¸Ñ Ð¼Ð¸ÐºÑÐ¾Ð ÐÐ Ð´Ð»Ñ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐ¸ Ð¸ Ð¼Ð¾Ð½Ð¸ÑÐ¾ÑÐ¸Ð½Ð³Ð° ÑÑÑÐµÐºÑÐ° ÑÐµÑÐ°Ð¿Ð¸Ð¸ Ð³ÐµÑÐ¼Ð¸Ð½Ð¾Ð³ÐµÐ½Ð½ÑÑ Ð¾Ð¿ÑÑ Ð¾Ð»ÐµÐ¹
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Development of a method for the analysis of circulating microRNAs for diagnostics and monitoring of the effect of therapy for germ cell tumors
Creators: name: ÐÐ½ÑÐ·ÐµÐ²Ð°, ÐÐ°ÑÐ³Ð°ÑÐ¸ÑÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: ÐÐ»ÐµÑÐºÐ° (Ð±Ð¸Ð¾Ð».)
ÐÐµÐ»ÐºÐ¸
ÐÐ¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð°Ñ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ñ
Ð³ÐµÑÐ¼Ð¸Ð½Ð¾Ð³ÐµÐ½Ð½ÑÐµ Ð¾Ð¿ÑÑ Ð¾Ð»Ð¸
ÑÐ°Ðº ÑÐ¸ÑÐµÐº
Ð¾Ð±ÑÐ°ÑÐ½Ð°Ñ ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸Ñ Ñ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐµÐ¹ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð½Ð¾Ð¹ ÑÐµÐ¿Ð½Ð¾Ð¹ ÑÐµÐ°ÐºÑÐ¸ÐµÐ¹
Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐ°
testicular cancer
germ cell tumor
RT-QPCR
diagnostics

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description: MicroRNAs are a class of small molecules that regulate gene expression at the posttranscriptional level. Many pathological conditions are accompanied by characteristic changes in the cellular profile of miRNA, which makes it possible to consider these molecules as a new promising class of biological markers. In this work, a method was developed for the analysis of circulating microRNAs for the diagnosis and monitoring of the effect of therapy for germ cell tumors. A new method for the specific isolation of microRNAs from plasma and a new approach to two-tailed-RT-PCR were used in this study. Reciprocal pairs of microRNAs have been developed for the primary diagnosis of TGCT and the differential diagnosis of seminoma and embryonic carcinoma. The levels of marker molecules were downregulated after treatment of the TGCT.