title: Optimization of kinetic and operating parameters in bioreactors using evolutionary algorithms
Creators: name: Barrera Hernández, Gonzalo Irving
Publisher: name: Instituto Tecnológico y de Estudios Superiores de Monterrey
Year: 2024
Subjects: subject: INGENIERÍA Y TECNOLOGÍA::CIENCIAS TECNOLÓGICAS::OTRAS ESPECIALIDADES TECNOLÓGICAS::OTRAS
subject: Technology
subject: Science
subject: Bioreactors
subject: Optimization
subject: Evolutionary algorithms
subject: Multi-objective optimization
subject: Kinetic parameters
subject: Operational variables
subject: Fed-batch process
subject: Escherichia coli
subject: Orange fluorescent protein (OFP)
subject: Biomass production
subject: Substrate consumption
subject: Aeration rate
subject: Genetic algorithms
subject: Biotechnology
subject: Bioprocess engineering

description: https://orcid.org/0000-0002-1099-8148
description: Bioreactors play a role in creating biological products such as medicines and biofuels by care fully controlling factors such as substrate levels and temperature within them to obtain optimal production results, bioreactor production process poses a challenge that poses a challenge to engineers due to the intricate setup involved. In the field of microbiology and biotechnology, conventional approaches such as the Monod model, logistic growth models, and fed-batch techniques have been employed to predict and improve the growth conditions of microor ganisms and the production of proteins of interest in fermenters. However, these approaches could face challenges when they encounter nonlinear systems and conflicting objectives. To address these challenges, our suggestion is to approach the configuration of factors in bioreactors as an optimization problem using an evolutionary algorithm that can improve the effectiveness and quality of the operating process. The objective of this study is to in vestigate and create a pipeline that integrates evolutionary algorithms to solve multi-objective and scalar optimization problems, aimed at identifying kinetic and critical parameters within a bioreactor system. The optimization process involves, in the first stage, a least squares ap proach that considers product, biomass, dissolved oxygen, and substrate concentrations as objectives, with the kinetic parameters (e.g., maximum specific growth rate and substrate affinity) serving as decision variables. The second stage focuses solely on maximizing the amount of produced product, specifically biomass, using critical operational variables, such as feed rate and aeration, as decision variables. The research employs Escherichia coli as a microorganism that has been genetically al tered to produce orange fluorescent protein (OFP) to test the validity of improvement frame works. Initially, in the simulation and process tuning phase, experimental information, from batch cultures, is used to accurately determine the factors. Later, in the fed-batch phase, the application of an algorithm is used to optimize biomass yield while considering operational constraints such as oxygen levels and maximum reactor volume. The findings show that this method accurately calculates factors during the fed-batch phase and efficiently increases biomass production in the continuous fed phase. The use of algorithms such as multiple NSGA-III and single-objective genetic algorithms provides valuable benefits when dealing with intricate bioreactor configurations that have conflicting objectives such as managing substrate consumption and improving production yield. This approach has promising prospects for improving the accuracy and efficiency of bioprocess optimization, while increasing its scalability, in the field of biotechnology in the future.