Additional file 3 of Astrocytes in CA1 modulate schema establishment in the hippocampal-cortical neuron network
Creators: name: Liu, Shu
name: Wong, Heung Yan
name: Xie, Li
name: Iqbal, Zafar
name: Lei, Zhuogui
name: Fu, Zhongqi
name: Lam, Yuk Yan
name: Ramkrishnan, Aruna Surendran
name: Li, Ying
Publisher: name: figshare
Year: 2022
Subjects: subject: Neuroscience
subject: Physiology
subject: FOS: Biological sciences
subject: Biological Sciences not elsewhere classified
subject: Science Policy

description: Additional file 3: Fig S3. CA1 astrocyte Gi activation impairs initial schema memory by inhibiting the activation of CA1-ACC projecting neurons (Calcium level). (A) Timeline of CNO/saline i.p., session 1 training and Calcium signals fiber photometry recording. (B) Representative Calcium signals transient of CA1-ACC projecting neurons during session 1 training of saline-injected (B) or CNO-injected (C); vertical axis, 10% ΔF/F%, horizontal axis, 10 s; gradient of heatmap, -5%-10% (ΔF/F%). (D) Probability distribution of averaged ΔF/F% of Ca2+ signals of CA1-ACC projecting neurons between hM4Di_Saline and hM4Di_CNO groups in session 1 (n=4, trial= 17 each). (E) Comparison between the averaged Ca2+ signals of CA1-ACC projecting neurons between hM4Di_Saline and hM4Di_CNO groups in session 1 (n=4, trial=17 each). (F) Representative Calcium signals transient of ACC neurons during session 1 training of saline-injected rats (F) or CNO-injected rats (G); vertical axis, 10% ΔF/F%, horizontal axis, 10 s; gradient of heatmap, 0%-6% (ΔF/F%). (H) Probability distribution of averaged ΔF/F% of Ca2+ signals of ACC neurons between hM4Di_Saline and hM4Di_CNO groups of rats in session 1 (n=4, trial= 16 in hM4Di_saline group, n=4 trials=18 in hM4Di_CNO group). (I) Comparison between the averaged Ca2+ signals of ACC neurons between hM4Di_Saline and hM4Di_CNO groups of rats in session 1 (n=4, trial= 16 in hM4Di_saline group, n=4 trials=18 in hM4Di_CNO group). (J) Left, schematic of CA1-NAc projecting neurons experiment: AAV2-retro-CaMKII-Cre was injected into the NAc, and AAV2/9-ef1α-DIO-EYFP together with AAV8-GFAP-hM4Di–mCherry were injected into the CA1; scale bar, 20 µm; Right, EYFP-positive axons of CA1 projection neurons in the NAc; scale bar, 100 µm. (K) Representative images of hM4Di in astrocytes (red), EYFP in NAc projecting CA1 neurons (green) and c-Fos (magenta) in the CA1 of saline-injected rats (K) or CNO-injected rats (L). (M) Representative images of c-Fos (red) in NAc of saline-injected rats (M) or CNO-injected (N). (O) Comparison of the percent of CA1 cells projecting into the NAc that express c-Fos between session 1 (n=5) and home-cage group (n=4). (P) Comparison of c-Fos expression level in NAc between session 1 (n=5) and home-cage group (n=4). (Q) Comparison of the percent of CA1 cells projecting into the NAc that express c-Fos between hM4Di_CNO and hM4Di_Saline groups (n=5 in hM4Di_Saline, n=4 in hM4Di_CNO group). (R) Comparison of c-Fos expression level in NAc between hM4Di_CNO (n=4) and hM4Di_Saline group (n=5). Probability distribution are presented as the mean ± SD, other data are presented as the mean ± SEM, *p&lt;0.05, ***p&lt;0.001, ns p&gt;0.05.