title: ÐÐ¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÐºÑÐ»ÑÑÑÑÐ°Ð»ÑÐ½ÑÑ ÑÑÐµÐ´ Chlorella vulgaris Ð´Ð»Ñ Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð±Ð¸Ð¾ÑÐ¸Ð½ÑÐµÐ·Ð° Ð±ÐµÐ»ÐºÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Modelling of culture media of Chlorella vulgaris for directed protein biosynthesis
Creators: name: Ð§Ð¸ÑÐºÐ¾Ð²Ð°, ÐÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Ð±ÐµÐ»Ð¾Ðº
Ð±Ð¸Ð¾ÑÐ¸Ð½ÑÐµÐ·
ÐºÑÐ»ÑÑÑÑÐ°Ð»ÑÐ½ÑÐµ ÑÑÐµÐ´Ñ
Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ð¸
Ð¼Ð¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
Ñ Ð»Ð¾ÑÐµÐ»Ð»Ð°
protein
biosynthesis
culture media
microalgae
modelling
chlorella

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÐºÑÐ»ÑÑÑÑÐ°Ð»ÑÐ½ÑÑ ÑÑÐµÐ´ Ð´Ð»Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð°ÑÐ¸Ð¸ Chlorella vulgaris Ñ ÑÐµÐ»ÑÑ Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð±Ð¸Ð¾ÑÐ¸Ð½ÑÐµÐ·Ð° Ð±ÐµÐ»ÐºÐ°. Ð¦ÐµÐ»ÑÑ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÐµÑÑÑ Ð¼Ð¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½ÑÑ ÑÑÐµÐ´ Ð´Ð»Ñ Ð²ÑÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ñ ÐºÑÐ»ÑÑÑÑÑ ÑÐ»Ð¾ÑÐµÐ»Ð»Ñ Ñ ÑÐµÐ»ÑÑ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¸Ð· Ð½ÐµÑ Ð¼Ð°ÐºÑÐ¸Ð¼Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð° Ð±ÐµÐ»ÐºÐ°, ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ½Ð°Ñ Ð¾ÑÐµÐ½ÐºÐ° ÑÐ¾ÑÑÐ° Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ, Ð°Ð½Ð°Ð»Ð¸Ð· Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½Ð¾Ð¹ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð½Ð° ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ðµ Ð±ÐµÐ»ÐºÐ°. ÐÐ°Ð´Ð°ÑÐ¸, ÑÐµÑÐ°ÐµÐ¼ÑÐµ Ð² ÑÐ¾Ð´Ðµ Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð¼ÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹: ÐÐ¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½ÑÑ ÑÑÐµÐ´ Ð´Ð»Ñ Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð±Ð¸Ð¾ÑÐ¸Ð½ÑÐµÐ·Ð° Ð±ÐµÐ»ÐºÐ°; 1) ÐÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÑÐ»Ð¾ÑÐµÐ»Ð»Ñ Ð½Ð° ÑÑÐ°Ð½Ð´Ð°ÑÑÐ½Ð¾Ð¹ ÑÑÐµÐ´Ðµ Ð¢Ð°Ð¼Ð¸Ñ Ð¸ Ð½Ð° Ð¼Ð¾Ð´ÐµÐ»ÑÐ½ÑÑ ÑÑÐµÐ´Ð°Ñ; 2) ÐÑÐµÐ½ÐºÐ° ÑÐ¾ÑÑÐ° Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÐ¼Ð¸ Ð¼ÐµÑÐ¾Ð´Ð°Ð¼Ð¸; 3) ÐÑÐµÐ½ÐºÐ° Ð¿ÑÐ¸ÑÐ¾ÑÑÐ° Ð±ÐµÐ»ÐºÐ° Ð² Ð·Ð°Ð´Ð°Ð½Ð½ÑÑ ÐºÑÐ»ÑÑÑÑÐ°Ð»ÑÐ½ÑÑ ÑÑÐ»Ð¾Ð²Ð¸ÑÑ; 4) ÐÐ±ÑÐ°Ð±Ð¾ÑÐºÐ° Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð´Ð°Ð½Ð½ÑÑ; 5) Ð ÐµÐºÐ¾Ð¼ÐµÐ½Ð´Ð°ÑÐ¸Ð¸ Ð¿Ð¾ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Chlorella vulgaris Ð² Ð¸ÑÐºÑÑÑÑÐ²ÐµÐ½Ð½ÑÑ ÑÑÐ»Ð¾Ð²Ð¸ÑÑ ÐÐ±ÑÐµÐºÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: Chlorella vulgaris. Ð Ð°Ð±Ð¾ÑÐ° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð² Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ ÐÐ¨ÐÐ¸ÐÐ, Ð³Ð´Ðµ Ð¸Ð·ÑÑÐ°Ð»Ð°ÑÑ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹ Ðº Ð½Ð°ÐºÐ¾Ð¿Ð»ÐµÐ½Ð¸Ñ Ð±ÐµÐ»ÐºÐ° Ð² Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÑÐ½Ð½ÑÑ Ð·Ð°Ð´Ð°Ð½Ð½ÑÑ ÑÑÐ»Ð¾Ð²Ð¸ÑÑ. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¾ Ð²ÑÑÑÐ½ÐµÐ½Ð¾, ÑÑÐ¾ ÑÐ»Ð¾ÑÐµÐ»Ð»Ð° ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð° Ð½Ð°ÐºÐ°Ð¿Ð»Ð¸Ð²Ð°ÑÑ Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐ¹ Ð±ÐµÐ»Ð¾Ðº. ÐÑÑÑÐ¸Ð¼Ð¸ ÑÑÐ»Ð¾Ð²Ð¸ÑÐ¼Ð¸ Ð´Ð»Ñ Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð±Ð¸Ð¾ÑÐ¸Ð½ÑÐµÐ·Ð° ÑÐ²Ð»ÑÐµÑÑÑ ÑÑÐµÐ´Ð° Ð¢Ð°Ð¼Ð¸Ñ Ñ Ð´Ð¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸ÐµÐ¼ Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð´Ð¾Ð±Ð°Ð²ÐºÐ¸ ÑÐºÑÑÑÐ°ÐºÑÐ° Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹ Ñ Ð³Ð»ÑÐºÐ¾Ð·Ð¾Ð¹. ÐÐ°ÐºÑÐ¸Ð¼Ð°Ð»ÑÐ½ÑÐ¹ Ð¿ÑÐ¸ÑÐ¾ÑÑ Ð±ÐµÐ»ÐºÐ° Ð½Ð°Ð±Ð»ÑÐ´Ð°Ð»ÑÑ Ð½Ð° Ð¿Ð¾ÑÐ»ÐµÐ´Ð½Ð¸Ðµ ÑÑÑÐºÐ¸ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¸ ÑÐ¾ÑÑÐ°Ð²Ð¸Ð» 56,19 % Ð´Ð¾Ð»Ñ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±ÑÐ°Ð·ÑÐ°, 76,85 % Ð´Ð»Ñ Ð¾Ð±ÑÐ°Ð·ÑÐ° Ñ Ð´Ð¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸ÐµÐ¼ Ð´ÑÐ¾Ð¶Ð¶ÐµÐ²Ð¾Ð³Ð¾ ÑÐºÑÑÑÐ°ÐºÑÐ° Ð¸ 62,55 % Ð´Ð»Ñ Ð¾Ð±ÑÐ°Ð·ÑÐ° Ñ Ð´Ð¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸ÐµÐ¼ Ð°Ð²ÑÐ¾Ð»Ð¸Ð·Ð°ÑÐ° Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹.
description: This work focuses on the study of culture media for the cultivation of Chlorella vulgaris for directed protein biosynthesis. The aim of this work is to simulate culture media for the cultivation of Chlorella vulgaris in order to extract the maximum amount of protein from it, a comprehensive evaluation of biomass growth, the analysis of the resulting biomass for protein content. Objectives of the research carried out: 1) Simulation of nutrient media for directed protein biosynthesis; 2) Cultivation of chlorella on standard Tamiya medium and on model media; 3) Evaluation of biomass growth by experimental methods; 4) Evaluation of protein growth under given culture conditions; 5) Data processing; 6) Recommendations for the cultivation of Chlorella vulgaris under artificial conditions. Subject: Chlorella vulgaris. This work was carried out in the laboratory of the Higher School of Biology and Pharmacology to study the ability of microalgae to accumulate protein under certain prescribed conditions. As a result of the study, it was found that Chlorella is capable of accumulating intracellular protein. The best conditions for directed biosynthesis are Tamiya medium with the addition of yeast extract with glucose as an additive. Maximum protein increase was observed on the last day of cultivation and was 56.19 % for the control sample, 76.85 % for the sample with addition of yeast extract and 62.55 % for the sample with addition of yeast autolysate