MOESM2 of High NCALD expression predicts poor prognosis of cytogenetic normal acute myeloid leukemia
Creators: name: Song, Ying
name: Zhang, Weilong
name: He, Xue
name: Liu, Xiaoni
name: Yang, Ping
name: Wang, Jing
name: Hu, Kai
name: Liu, Weiyou
name: Zhang, Xiuru
name: Jing, Hongmei
name: Yuan, Xiaoliang
Publisher: name: figshare
Year: 2019
Subjects: subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Cancer
subject: Science Policy
subject: Hematology

description: Additional file 2: Figure S1. High NCALD expression predicts worse survival of CN-AML. The X axis represents time (months) and the Y axis represents survival probability. Kaplan-Meier curves were used for OS in different NCALD expression groups of CN-AML patients from GSE71014. The cutoff values for the high and low NCALD groups were 6.8281. OS, P &lt; 0.0001; log rank test. Figure S2. High NCALD expression predicts poor survival of CN-AML. The X axis represents time and the Y axis represents survival probability. All patients were divided into two groups based on quartiles of NCALD expression levels. Top 75% patients are NCALD high expression group and the other 25% patients are NCALD low expression group by ranking NCALD gene expression from high to low. A, Kaplan-Meier curves were used for EFS and OS in different NCALD expression groups of CN-AML in the TCGA dataset. EFS, P &lt; 0.0001; OS, P = 0.00035; log rank test. B, Kaplan-Meier curves were used for OS in different NCALD expression groups of CN-AML in GSE12417. OS, P &lt; 0.0001; log rank test. C, Kaplan-Meier curves were used for OS in different NCALD expression groups in GSE22778. OS, P &lt; 0.0001; log rank test. Figure S3. High NCALD expression predicts poor survival of CN-AML. The X axis represents time (months) and the Y axis represents survival probability. All patients were divided into two groups based on quartiles of NCALD expression levels. Top 75% patients are NCALD high expression group and the other 25% patients are NCALD low expression group by ranking NCALD gene expression from high to low. Kaplan-Meier curves were used for OS in different NCALD expression groups of CN-AML patients from GSE71014. OS, P &lt; 0.0001; log rank test. Figure S4. High NCALD expression predicts poor survival of AML patients after allo-HSCT or chemotherapy from the TCGA dataset. The X axis represents time (months) and the Y axis represents survival probability. All patients were divided into two groups based on quartiles of NCALD expression levels. Top 75% patients are NCALD high expression group and the other 25% patients are NCALD low expression group by ranking NCALD gene expression from high to low. A, Kaplan–Meier curves were used of EFS and OS in different NCALD expression groups after allogeneic hematopoietic stem cell transplantation (allo-HSCT). EFS, P = 0.0051; OS, P = 0.028; log rank test. B, Kaplan–Meier curves were used of EFS and OS in different NCALD expression group after chemotherapy. EFS, P = 0.011; OS, P = 0.01; log rank test. Figure S5. The NCALD gene is the most significant predictor of TCGA CN-AML survivals. The X axis represents the hazard ratio (log2 HR) and the Y axis represents the P-value (−log10). A, HR and P-value scatter plots for EFS univariate analysis of all variables (17 variables). The lower and upper 95% confidence interval of HR was showed in the scatter plot. B, HR and P-value scatter plots for OS univariate analysis of all variables (17 variables). The lower and upper 95% confidence interval of HR was showed in the scatter plot. Figure S6. ROC curves of survival for age in TCGA CN-AML patients. The X axis represents false positive (FP) and the Y axis represents true positive (TP). A, ROC curves for age and EFS of 75 TCGA CN-AML patients were performed. AUC reached 0.559 (1 year survival, left panel), 0.551 (2 year survival, middle panel) and 0.591 (3 year survival, right panel) respectively. B, ROC curves for age and OS of 75 TCGA CN-AML patients were performed. AUC reached 0.605 (1 year survival, left panel), 0.62 (2 year survival, middle panel) and 0.605 (3 year survival, right panel) respectively. The grey shadow of the curves represents 95% confidence interval. Figure S7. ROC curves of survival for WBC count in TCGA CN-AML patients. The X axis represents false positive (FP) and the Y axis represents true positive (TP). A, ROC curves for WBC count and EFS of 75 CN-AML TCGA patients were performed. AUC reached 0.598 (1 year survival, left panel), 0.633 (2 year survival, middle panel) and 0.604 (3 year survival, right panel) respectively. B, ROC curves for WBC count and OS of 75 CN-AML TCGA patients were performed. AUC reached 0.56 (1 year survival, left panel), 0.624 (2 year survival, middle panel) and 0.616 (3 year survival, right panel) respectively. The grey shadow of the curves represents 95% confidence interval. Figure S8. ROC curves of survival for DNMT3A mutation in TCGA CN-AML patients. The X axis represents false positive (FP) and the Y axis represents true positive (TP). A, ROC curves for DNMT3A mutation and EFS of 75 CN-AML TCGA patients were performed. AUC reached 0.639 (1 year survival, left panel), 0.64 (2 year survival, middle panel) and 0.643 (3 year survival, right panel) respectively. B, ROC curves for DNMT3A mutation and OS of 75 CN-AML TCGA patients were performed. AUC reached 0.641 (1 year survival, left panel), 0.599 (2 year survival, middle panel) and 0.622 (3 year survival, right panel) respectively. The grey shadow of the curves represents 95% confidence interval. Figure S9. Expression level of NCALD gene in different subtypes of AML. The X axis represents different subtypes and the Y axis represents gene expression. NCALD gene expression was measured as log2. A, Comparison of NCALD expression levels in four subtypes of AML, P = 0.00019; ANOVA, ns, ** and *** indicate P &gt; 0.05, P ≤ 0.01 and P ≤ 0.001, respectively. Each group is compared to the average of the entire data. Add a horizontal dashed line at the average. B, High expression level of NCALD gene in LSC+, P = 0.01; unpaired t test, two sided.