title: ÐÐ¾Ð²ÑÑÐµÐ½Ð¸Ðµ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð°Ð½ÑÐ¸Ð¼Ð¸ÐºÑÐ¾Ð±Ð½ÑÑ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð² Ð¿ÑÑÐµÐ¼ Ð¸Ð½ÐºÐ°Ð¿ÑÑÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð² Ð»Ð¸Ð¿Ð¾ÑÐ¾Ð¼Ñ ÑÐ¾ÑÑÐ¾Ð»Ð¸Ð¿Ð¸Ð´Ð¾Ð²
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Increasing the efficiency of antimicrobial drugs by encapsulation of phospholipids into liposomes
Creators: name: ÐÐµÐ²Ð¸Ð½, ÐÐ³Ð¾ÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÐÐ½ÑÐ¸Ð±Ð¸Ð¾ÑÐ¸ÐºÐ¸
Ð°Ð½ÑÐ¸Ð±Ð¸Ð¾ÑÐ¸ÐºÐ¾ÑÐµÐ·Ð¸ÑÑÐµÐ½ÑÐ½Ð¾ÑÑÑ
Ð»Ð¸Ð¿Ð¾ÑÐ¾Ð¼Ñ
Ð½Ð°Ð½Ð¾ÐºÐ¾Ð½ÑÐµÐ¹Ð½ÐµÑÑ
ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ðµ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð°Ð½ÑÐ¸Ð±Ð¸Ð¾ÑÐ¸ÐºÐ¾Ð²
antibiotic resistance
liposomes
nanocontainers
increased antibiotics efficiecy

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description: The theme of the final qualification work: "Improving the effectiveness of antimicrobial drugs by encapsulating phospholipids in liposomes". This work is devoted to the study of the possibility of increasing the effectiveness of antimicrobial drugs by encapsulating them in liposomes of phospholipids. Tasks that were solved in the course of the study: 1. Based on literature data, identify the advantages and disadvantages of the methods used to create liposomal capsules; 2. Explore approaches to the creation of liposomes as structures that deliver antibiotics to the site of action; 3. Show the effectiveness of the use of an encapsulated antibiotic in relation to bacterial cells. The work was carried out at the Cherkizovo Scientific and Research Center, where a significant part of the factual material was performed: a liposomal suspension of levofloxacin was created and its effectiveness was evaluated. Liposomes for antibiotic loading were prepared in two steps, by thin lipoid film hydration and then by sonication. The loading of levoflaxacin was carried out by the method of creating a transmembrane gradient of ammonium sulfate. Efficacy was evaluated by co-incubation of the resulting liposomal suspension and the classic form of levofloxacin. As a result, a liposomal suspension of levofloxacin was created and its effectiveness was evaluated. Based on the conducted studies, it can be concluded that the liposomal form of levofloxacin contributes to a decrease in cell viability by 27.4% compared to the classical form.