cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Development of iminosugar-based glycosidase inhibitors as drug candidates for SARS-CoV-2 virus via molecular modelling and in vitro studies
Creators: name: Ferjancic, Zorana
name: Bihelovic, Filip
name: Vulovic, Bojan
name: Matovic, Radomir
name: Trmcic, Milena
name: Jankovic, Aleksandar
name: Pavlovic, Milos
name: Djurkovic, Filip
name: Prodanovic, Radivoje
name: Djelmas, Aleksandra Djurdjevic
name: Kalicanin, Nevena
name: Zlatovic, Mario
name: Sladic, Dusan
name: Vallet, Thomas
name: Vignuzzi, Marco
name: Saicic, Radomir N.
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Biochemistry
subject: Pharmacology
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Information Systems not elsewhere classified
subject: Cancer
subject: Mental Health
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Virology

description: We developed new iminosugar-based glycosidase inhibitors against SARS-CoV-2. Known drugs (miglustat, migalastat, miglitol, and swainsonine) were chosen as lead compounds to develop three classes of glycosidase inhibitors (α-glucosidase, α-galactosidase, and mannosidase). Molecular modelling of the lead compounds, synthesis of the compounds with the highest docking scores, enzyme inhibition tests, and in vitro antiviral assays afforded rationally designed inhibitors. Two highly active α-glucosidase inhibitors were discovered, where one of them is the most potent iminosugar-based anti-SARS-CoV-2 agent to date (EC90 = 1.94 µM in A549-ACE2 cells against Omicron BA.1 strain). However, galactosidase inhibitors did not exhibit antiviral activity, whereas mannosidase inhibitors were both active and cytotoxic. As our iminosugar-based drug candidates act by a host-directed mechanism, they should be more resilient to drug resistance. Moreover, this strategy could be extended to identify potential drug candidates for other viral infections.
Low molecular weight cellulose ethers as aerosols for the the consolidation of cohesively weak paint layers
Creators: name: Stahmann, Charlotte N.
name: Soppa, Karolina
name: Glüsen, Birgit
name: Storevik-Tveit, Eva
name: Käckel, Andreas
name: Demuth, Petra
Year: 2022

description: Due to Edvard Munch’s (1863-1944) unconventional painting technique, choice of materials, and the unstable climate conditions of his studios, where the paintings were stored during his lifetime, many of his paintings, now housed at MUNCH, have cohesively weak and loose paint layers. As a result, consolidation and re-adhesion of these fragile paint layers are the most frequently performed conservation treatments on his paintings. A selection of low molecular weight (lmw) hydroxypropyl methylcellulose ethers (HPMC), new to the field of conservation, have been evaluated in comparison to methylcellulose (MC) A4C and sturgeon glue regarding their suitability for the consolidation of cohesively weak paint layers. The mock-ups used for these investigations were of a similar composition (pigment, binding medium and pigment-binding medium ratio) and porosity to a paint sample from the painting “Beach Landscape with Trees and Boats” from 1905-06 by Edvard Munch. Viscosity and surface tension of aqueous solutions of the consolidants and their influence on the imbibition time and depth into porous paint layers were investigated. Fluorescence labelling was used to visualize the imbibition depth of an aqueous solution of the lmw HPMC E3 and MC A4C, applied as an aerosol. With this method it could be shown that the applied amount and the application method of the consolidant (with or without intermediate drying steps) can play a crucial role in the imbibition depth. To evaluate the consolidation effect of the tested polymers, the aerosols of their aqueous solutions were applied on the paint mock-ups in a reproducible and standardized way, using an automated two-axis-table. A customised abrasion test was developed to evaluate the comparative increase of the paint layer cohesion after consolidation. These preliminary investigations show lmw HPMC as promising alternatives to established consolidants. They allow for an ultrasonic nebulisation in higher concentrations and thus for the paint layer’s consolidation in a lower number of applications.
Identification of potential inhibitors against Staphylococcus aureus shikimate dehydrogenase through virtual screening and susceptibility test
Creators: name: Zhu, Mengfan
name: Qu, Jinfeng
name: Deng, Qi
Publisher: name: Taylor & Francis
Year: 2024
Subjects: subject: Biochemistry
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Pharmacology
subject: Biotechnology
subject: Environmental Sciences not elsewhere classified
subject: Chemical Sciences not elsewhere classified
subject: Cancer
subject: Hematology
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Virology

description: Staphylococcus aureus shikimate dehydrogenase (SaSDH) plays a crucial role in the growth of Staphylococcus aureus (S. aureus), but absent in mammals and therefore a potential target for antibacterial drugs to treat drug-resistant S. aureus infection. In this study, a 3D model of SaSDH was constructed by homology modelling and inhibitors of SaSDH were screened through virtual screening. (-)-Gallocatechin gallate and rhodiosin were identified as inhibitors with Kis of 2.47 μM and 73.38 μM, respectively. Molecular docking and isothermal titration calorimetry showed that both inhibitors interact with SaSDH with a KD of 44.65 μM for (-)-gallocatechin gallate and 16.45 μM for rhodiosin. Both inhibitors had antibacterial activity, showing MICs of 50 μg/mL for (-)-gallocatechin gallate and 250 μg/mL for rhodiosin against S. aureus. The current findings have the potential for identification of drugs to treat S. aureus infections by targeting SaSDH.
Antifungal and anti-biofilm effects of hydrazone derivatives on Candida spp
Creators: name: Popczyk, Pierre
name: Ghinet, Alina
name: Bortolus, Clovis
name: Kamus, Laure
name: Lensink, Marc F.
name: de Ruyck, Jérôme
name: Sendid, Boualem
name: Dubar, Faustine
Publisher: name: Taylor & Francis
Year: 2024
Subjects: subject: Biochemistry
subject: Pharmacology
subject: Biotechnology
subject: Ecology
subject: FOS: Biological sciences
subject: Biological Sciences not elsewhere classified
subject: Science Policy
subject: Infectious Diseases
subject: FOS: Health sciences

description: Worldwide, invasive candidiasis are a burden for the health system due to difficulties to manage patients, to the increasing of the resistance of the current therapeutics and the emergence of naturally resistant species of Candida. In this context, the development of innovative antifungal drugs is urgently needed. During invasive candidiasis, yeast is submitted to many stresses (oxidative, thermic, osmotic) in the human host. In order to resist in this context, yeast develops different strategy, especially the biosynthesis of trehalose. Starting from the 3D structural data of TPS2, an enzyme implicated in trehalose biosynthesis, we identified hydrazone as an interesting scaffold to design new antifungal drugs. Interestingly, our hydrazone derivatives which demonstrate antifungal and anti-biofilm effects on Candida spp., are non-toxic in in vitro and in vivo models (Galleria mellonella).
Lutjanus quinquelineatus
Creators: name: Swain, Ritanjali
name: Kisku, Subash
name: Nayak, Lakshman
name: Mishra, Subhrendu Sekhar
name: Patro, Shesdev
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Chordata
Perciformes
Lutjanidae
Lutjanus
Lutjanus quinquelineatus

description: 3. Lutjanus quinquelineatus (Bloch, 1790): Five-lined Snapper (Figure 3)
Material examined: EBEC /ZSI/F-13366, 1 ex., 175 mm SL, Gopalpur fish landing center, coll. R. Swain, 27 th October 2021.
Diagnosis: D X, 14; A III, 8; P 16; V 1, 5; LL 60; GL 10+10. Body depth 2.73, head length 2.64 times in SL; eye diameter 3.31, suborbital depth 5.84 times in HL; less than eye diameter. Preopercular notch and knob well developed; vomerine tooth patch crescentic without a medial posterior extension; tongue smooth, without teeth; scale rows above lateral line rise obliquely; caudal fin emarginated. Colour: Body bright yellow; brownish dorsally; five bright blue stripes on sides; a round black spot on lateral line below soft part of dorsal fin; fins yellow.
Distribution: Indo-West Pacific: from Persian Gulf and the Gulf of Oman to Fiji, north to southern Japan, south to New South Wales (Australia) and Lord Howe Island (Froece & Pauly, 2021). India – Kerala (Pillay, 1929), Tamil Nadu (Murty, 1969), Andhra Pradesh (Barman et al., 2004), West Bengal (Ray et al., 2017), Andaman & Nicobar Islands (Day, 1871, as Genyoroge grammica, a synonym) and Odisha (present report).
Remarks: This species is known to have distributed along coasts of both the neighboring states, West Bengal in the north (Ray et al., 2017) and Andhra Pradesh in the south (Barman et al., 2004) along the east coast. It was probably confused with Lutjanus kasmira (Fabricius, 1775) in earlier works which is known to occur along the Odisha coast. The present report forms its first record from the Odisha coast.
description: Published as part of Swain, Ritanjali, Kisku, Subash, Nayak, Lakshman, Mishra, Subhrendu Sekhar & Patro, Shesdev, 2022, First record of four snapper fishes (Perciformes: Lutjanidae) from Odisha, East coast of India, pp. 43-49 in Records of the Zoological Survey of India 122 (1) on pages 44-45, DOI: 10.26515/rzsi/v122/i1/2022/167436, http://zenodo.org/record/10913879
Lutjanus vitta
Creators: name: Swain, Ritanjali
name: Kisku, Subash
name: Nayak, Lakshman
name: Mishra, Subhrendu Sekhar
name: Patro, Shesdev
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Chordata
Perciformes
Lutjanidae
Lutjanus
Lutjanus vitta

description: 4. Lutjanus vitta (Quoy & Gaimard, 1824): Blackstriped snapper (Figure 4)
Material examined: EBEC /ZSI/F-13370, 1 ex., 239 mm SL, Gopalpur fish landing center, coll. R. Swain, 25 th November 2021.
Diagnosis: D X, 14; A III, 9; P 15; V 1, 5; LL 52; GL 9+10. Body depth 2.91 times and HL 2.67 times in SL; eye diameter 4.09 times and suborbital depth7.20 times in HL; preopercular notch and knob poorly developed; vomerine tooth patch crescentic without a medial posterior extension; tongue smooth, without teeth; 9 transverse scale rows on cheek; caudal fin slightly emarginated; scale rows oblique above lateral line but horizontal below. Colour: Dorsal profile of body brown with a greenish tinge, lower sides and abdomen pinkish white, with a dark broad median lateral stripe on sides.
Distribution: Indo-West Pacific: Seychelles, Sri Lanka to New Caledonia, New Ireland (Papua New Guinea) and Gilbert Islands, northward to the Ryukyu Islands, Japan (Froece & Pauly, 2021). India – Andhra Pradesh (Barman et al., 2004), Tamil Nadu (Krishnan et al., 2007), Gujarat (Barman et al., 2000), Maharashtra (Barman et al., 2012), Karnataka (Barman et al., 2013), Kerala (Barman, 2017), Lakshadweep (Barman et al., 2014), Andaman & Nicobar Islands (Rao et al., 2000) and Odisha (present report).
Remarks: This species is reported from the southern neighboring state, Andhra Pradesh (Barman et al., 2004) most probably based on the distribution map given in Allen (1985), however it is not known to occur along West Bengal coast (Barman, 2017) to the north of Odisha. The present paper reports Lutjanus vitta as first record from the Odisha coast with material evidence.
description: Published as part of Swain, Ritanjali, Kisku, Subash, Nayak, Lakshman, Mishra, Subhrendu Sekhar & Patro, Shesdev, 2022, First record of four snapper fishes (Perciformes: Lutjanidae) from Odisha, East coast of India, pp. 43-49 in Records of the Zoological Survey of India 122 (1) on pages 45-46, DOI: 10.26515/rzsi/v122/i1/2022/167436, http://zenodo.org/record/10913879
Additional file 1: of
                     P16-specific DNA methylation by engineered zinc finger methyltransferase inactivates gene transcription and promotes cancer metastasis
Creators: name: Chenghua Cui
name: Gan, Ying
name: Liankun Gu
name: Wilson, James
name: Zhaojun Liu
name: Baozhen Zhang
name: Dajun Deng
Publisher: name: Figshare
Year: 2015
Subjects: subject: Biochemistry
subject: Space Science
subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Plant Biology

description: Figures S1 to S6. (DOC 3228 kb)
Täglich zwischen Physiologie und Medikalisierung
Creators: name: Zaugg, Sibil
name: Antener, Sophie
name: Origlia Ikhilor, Paola
Publisher: name: Schweizerischer Hebammenverband (SHV)
Year: 2019

description: Die Geburt in den Kliniken ist zuweilen stark medikalisiert, während bei Hebammen ein von Physiologie geprägtes Berufsverständnis vorherrscht. Eine Bachelorthesis untersuchte, inwiefern Hebammen ein solches Spannungsfeld wahrnehmen und ob es einen Einfluss auf ihre Arbeitszufriedenheit hat. Sie berichteten über einen aufreibenden Kampf für die Physiologie, fragwürdige Indikationen und eine wenig frauenzentrierte Betreuung aufgrund einer vorgegebenen Spitalroutine.
Additional file 1: of Early diagnosis of gestational trophoblastic neoplasia based on trajectory classification with compartment modeling
Creators: name: Burny, Claire
name: Rabilloud, Muriel
name: FranĂ§Ois Golfier
name: JĂŠrĂ´Me Massardier
name: Hajri, Touria
name: Schott, Anne-Marie
name: Subtil, Fabien
Publisher: name: Figshare
Year: 2016
Subjects: subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics

description: Simulation design and results. (PDF 101 kb)
MOESM1 of BoHV-4-based vector delivering Ebola virus surface glycoprotein
Creators: name: Rosamilia, Alfonso
name: Jacca, Sarah
name: Tebaldi, Giulia
name: Tiberti, Silvia
name: Franceschi, Valentina
name: Macchi, Francesca
name: Cavirani, Sandro
name: Kobinger, Gary
name: Knowles, Donald
name: Donofrio, Gaetano
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biophysics
subject: Biochemistry
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: 60506 Virology
subject: FOS: Biological sciences
subject: Computational Biology

description: Additional file 1: Figure S1. Overall strategy employed for cloning and expression of EBOV GP as a soluble secreted form. A Diagram (not to scale) showing the structure of gD106 tagged (red) EBOV GP. The EBOsecgD106 has been obtained by eliminating the transmembrane domain (TM). EBOsecgD106 peptide has been produced in serum free medium of HEK 293T cells transfected with pCMVEBOsecgD106. B A time course to optimize EBOsecgD106 expression at different time post transfection as analyzed by western immunoblotting was used. Twenty-four hours was considered as the best time post transfection to collect the cell sovranatant.
Additional file 9: Figure S3. of Automatically visualise and analyse data on pathways using PathVisioRPC from any programming environment
Creators: name: Anwesha Bohler
name: Eijssen, Lars
name: Iersel, Martijn Van
name: Leemans, Christ
name: Willighagen, Egon
name: Kutmon, Martina
name: Jaillard, Magali
name: Evelo, Chris
Publisher: name: Figshare
Year: 2015
Subjects: subject: Space Science
subject: Genetics
subject: FOS: Biological sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Inorganic Chemistry
subject: FOS: Chemical sciences
subject: Plant Biology
subject: 60506 Virology
subject: Computational Biology

description: Oxidative stress pathway [52] for bone marrow cells showing the logFC and P-value for day 1, day 2, and day 5. (PDF 105Â kb)
Expanding the antiprotozoal activity and the mechanism of action of n-butyl and iso-butyl ester of quinoxaline-1,4-di-N-oxide derivatives against Giardia lamblia, Trichomonas vaginalis, and Entamoeba histolytica. An in vitro and in silico approach
Creators: name: González-González, Alonzo
name: Sánchez-Sánchez, Oscar
name: Yépez-Mulia, Lilián
name: Delgado-Maldonado, Timoteo
name: Vázquez-Jiménez, Lenci K.
name: López-Velázquez, Gabriel
name: la Mora, José Ignacio de la Mora-de
name: Pacheco-Gutierrez, Sebastian
name: Chino-Ríos, Laura
name: Arias, Diego
name: Moreno-Rodríguez, Adriana
name: Paz-González, Alma
name: Ortíz-Pérez, Eyra
name: Rivera, Gildardo
Publisher: name: Taylor & Francis
Year: 2024
Subjects: subject: Biochemistry
subject: Space Science
subject: Physical Sciences not elsewhere classified
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Pharmacology
subject: Chemical Sciences not elsewhere classified
subject: Science Policy
subject: Biological Sciences not elsewhere classified
subject: Infectious Diseases
subject: FOS: Health sciences

description: In this study, n-butyl and iso-butyl quinoxaline-7-carboxylate-1,4-di-N-oxide derivatives were evaluated in vitro against Giardia lamblia (G. lamblia), Trichomonas vaginalis (T. vaginalis), and Entamoeba histolytica (E. histolytica). The potential mechanism of action determination was approached by in silico analysis on G. lamblia and T. vaginalis triosephosphate isomerase (GlTIM and TvTIM, respectively), and on E. histolytica thioredoxin reductase (EhTrxR). Enzyme inactivation assays were performed on recombinant GlTIM and EhTrxR. Compound T-167 showed the best giardicidal activity (IC50 = 25.53 nM) and the highest inactivation efficiency against GlTIM without significantly perturbing its human homolog. Compounds T-142 and T-143 showed the best amoebicidal (IC50 = 9.20 nM) and trichomonacidal (IC50 = 45.20 nM) activity, respectively. Additionally, T-143 had a high activity as giardicial (IC50 = 29.13 nM) and amoebicidal (IC50 = 15.14 nM), proposing it as a broad-spectrum antiparasitic agent. Compounds T-145, and T-161 were the best EhTrxR inhibitors with IC50 of 16 µM, and 18 µM, respectively.
Additional file 5: Figure S1. of Automatically visualise and analyse data on pathways using PathVisioRPC from any programming environment
Creators: name: Anwesha Bohler
name: Eijssen, Lars
name: Iersel, Martijn Van
name: Leemans, Christ
name: Willighagen, Egon
name: Kutmon, Martina
name: Jaillard, Magali
name: Evelo, Chris
Publisher: name: Figshare
Year: 2015
Subjects: subject: Space Science
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics
subject: Marine Biology

description: Box plots showing array quality control and normalisation results. Box plots showing array quality control and normalization results using different normalization methods (BGcorrected, Loess, Loess + Scale, Loess + Quantile). (PDF 424 kb)
Neolithic and Bronze Age Archery Equipment from Alpine Ice-Patches: A Review on Components, Construction Techniques and Functionality
Creators: name: Junkmanns, Jürgen
name: Klügl, Johanna
name: Schoch, Werner
name: Di Pietro, Giovanna
Year: 2019
Subjects: prehistoric archery equipment
ice-patch archaeology
glacial archaeology
Neolithic
Bronze Age
Alps

description: The alpine ice-patch sites of Tisenjoch (I), Schnidejoch and Loetschenpass (CH) brought to light the most complete archery equipment known from European Prehistory. From the end of the last glaciation until the Middle Ages, bows and arrows were the most important weapons for hunting and warfare. The first verified artefacts of archery equipment are the arrows from Stellmoor, Northern Germany, which date to 10,000 BC, while the oldest bows found so far are still the two elm bows from Holmegard in Southern Denmark, dated to ca. 8000 – 6500 BC (Junkmanns 2013). During the Neolithic, bows were made almost exclusively from yew wood (Taxus baccata). Despite their different shapes, all prehistoric bows found in Europe are simple man-tall bows made from a single piece of wood with a more or less D-shaped cross-section and a flat belly side. Arrows were made from split wood or thin saplings and equipped with different types of points made from stone, bone/ antler material or the wood itself, according to their specific intended purpose. The manufacturing process can be described from several finds of unfinished bow blanks, as in the case of the Tisenjoch finds. Neolithic arrows were made from shoots of hazel (Corylus avellana), guelder rose (Viburnum sp.) or other hardwoods. They were straightened by heat and are generally longer and thicker than modern sporting arrows for increased weight and penetration power. Their fletching with three split feathers is practically the same as fletching used today. Bowstrings are extremely rare in European archaeological sites. Only two assured samples, stemming from the Tisenjoch and the Schnidejoch ice-patches, are known to date. They were made from animal sinew fibres, which are not preserved in non-frozen sites. Although there was almost certainly a need for a cover to protect a bow against bad weather, there is only one example of a Neolithic bow case known to date. The cover, made from water resistant birch bark measuring a little longer than the bow carried inside it, was found on Schnidejoch. It incorporates a carrying system of leather straps, which enabled the user to wear it over the shoulder, keeping the hands free for other tasks. It is supposed that other bow cases, which very probably existed in the Neolithic, were made from animal hide or leather which did not survive in waterlogged sites. That there were protective carrying devices for archery gear is also generally testified by the leather arrow quiver found on Tisenjoch and by numerous other ethnographic and historic examples.
MOESM2 of BoHV-4-based vector delivering Ebola virus surface glycoprotein
Creators: name: Rosamilia, Alfonso
name: Jacca, Sarah
name: Tebaldi, Giulia
name: Tiberti, Silvia
name: Franceschi, Valentina
name: Macchi, Francesca
name: Cavirani, Sandro
name: Kobinger, Gary
name: Knowles, Donald
name: Donofrio, Gaetano
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biochemistry
subject: Molecular Biology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Ecology
subject: FOS: Biological sciences
subject: Cancer
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: 60506 Virology

description: Additional file 2: Figure S2. Incorporation of EBOV GP into recombinant BoHV-4 particles. Extracts of purified viruses (BoHV-4-A and BoHV-4-syEBOVgD106) analyzed by Western immunoblotting.
Die maximal tragbare Radlast – eine zweckmässige Kenngrösse für die Praxis
Creators: name: Chervet, A.
name: Sturny, W.
name: Gut, S.
name: Sommer, M.
name: Stettler, Matthias
name: Weisskopf, P.
name: Keller, Th.
Publisher: name: Agroscope Liebefeld-Posieux ALP
Year: 2016
Subjects: soil compaction
precompression stress
trafficability
soil matric potential
no-tillage
mouldboard ploughing

description: Hohe Radlasten führen vor allem in Unterböden zu Druckbelastungen, die dauerhafte Verformungen und damit Beeinträchtigungen der Bodenfunktionen und der Ertragsfähigkeit verursachen. Die maximal tragbare Radlast ist jene Radlast, bei der die Bodenbelastung gerade noch unterhalb der Bodenfestigkeit (bestimmt als Vorbelastung) liegt und die Bodenstruktur deshalb noch nicht dauerhaft verformt wird. Wir berechneten die saisonalen Veränderungen der maximal tragbaren Radlast für die beiden Anbausysteme Direktsaat und Pflug sowie für den Dauergrünlandstreifen zwischen den Versuchsparzellen der Dauerbeobachtungsfläche Oberacker (sandiger Lehm). Gemessen wurde die Bodenfeuchtigkeit (als Matrixpotenzial) in situ und die Vorbelastung bei verschiedenen Matrixpotenzialen an ungestörten Zylinderproben im Labor. Die Simulationen wurden für eine Referenztiefe von 35 cm sowohl für Standardreifen als auch für Niederdruckreifen durchgeführt. Es zeigte sich, dass sowohl die Vorbelastung als auch die maximal tragbare Radlast stark von der Bodenfeuchtigkeit abhängig sind. Bei Niederdruckreifen ist die maximal tragbare Radlast höher als bei Standardreifen. Die Anzahl Tage, an denen der Boden ohne Verdichtungsrisiko befahren werden kann, schwankt stark von Jahr zu Jahr, ist beim Pflugsystem leicht höher als beim Direktsaatsystem und nimmt mit steigender Radlast ab. Die Darstellung des Verdichtungsrisikos mit dem Parameter «maximal tragbare Radlast» ist einfach zu interpretieren und deshalb nützlich für die Prävention von Bodenverdichtungen.
Sire-feed interactions for fattening performance and meat quality traits in growing-finishing pigs under a conventional and an organic feeding regimen
Creators: name: Quander-Stoll, Nele
name: Früh, Barbara
name: Bautze, David
name: Zollitsch, Werner
name: Leiber, Florian
name: Scheeder, Martin
Publisher: name: Elsevier
Year: 2021
Subjects: Organic pig production
Genotype-environment interactions
Sire lines
Protein sources
Feed efficiency
Lipid composition

description: In a two-factorial feeding trial 120 growing-finishing pigs from eleven sires were fed on an organic (ORG) or a conventional (CON) diet. Diet ORG contained mainly oil press cakes and legume grains as protein source containing higher protein and crude fiber content along with slight deficiencies of limiting amino acids. Pigs were allocated to treatments balanced according to litter, sex and initial weight. Feed was offered ad libitum. Feed consumption, weight gain as well as carcass, meat and fat quality traits were recorded. ORG fed animals had lower weight gain, poorer feed conversion, lower loin muscle area, higher intramuscular fat content, higher ultimate pH (loin, ham), and a higher PUFA content in backfat. Despite for cook loss and dressing percentage, no sire-feed interactions were found. This indicates no need for a performance test, specifically designed for organic production. However, weight of the breeding values for the various traits and selection criteria should be adapted to the needs of organic production.
Dilution rates of cattle slurry affect ammonia uptake and protein production of duckweed grown in recirculating systems
Creators: name: Stadtlander, Timo
name: Bandy, Jaclyn
name: Rosskothen, Dennis
name: Pietsch, Constanze
name: Tschudi, Fridolin
name: Sigrist, Mathias
name: Seitz, Andreas
name: Leiber, Florian
Publisher: name: Elsevier
Year: 2022
Subjects: Duckweed
Cow slurry
total ammonia nitrogen
biomass and protein production

description: Duckweed is a potential collector of nitrogen from animal liquid manure and a source of protein suitable as feed for livestock and fish. Therefore, it provides opportunities for circular economy systems. Two duckweed species, Spirodela polyrhiza and Landoltia punctata, were grown in five recirculating systems each connected to a reservoir tank filled with water and graded organic cattle slurry concentrations. Fresh and dry biomass, protein production and amino acid profiles among the nitrogen removal were evaluated. Spirodela polyrhiza showed a significantly higher fresh biomass production but L. punctata dry matter content was generally higher resulting in similar dry biomass production for both species This study shows clearly that the crude protein content, ranging between 29.3 and 37.9% of dry matter, was positively correlated to slurry and total ammonia nitrogen (TAN) concentration of the substrate, independent of the duckweed species. Total crude protein yield was in the range of 1.37–1.95 g/m2/d, following a quadratic function regarding slurry and TAN concentrations, with marginal differences between species. Biomass and crude protein yields were optimal for both duckweed species at a TAN concentration of 19 mg/l, which corresponded to a slurry dilution of 1:8. The results of this study provide important information for operation of recirculating duckweed production systems on slurry and operators should aim to keep TAN concentrations in that range for optimization of protein production in conjunction with TAN removal.
Additional file 2: of Effect of herd size on subclinical infection of swine in Vietnam with influenza A viruses
Creators: name: Takemae, Nobuhiro
name: Yugo Shobugawa
name: Nguyen, Phuong
name: Nguyen, Tung
name: Nguyen, Tien
name: To, Thanh
name: Thai, Phuong
name: Nguyen, Tho
name: Nguyen, Duy
name: Nguyen, Dung
name: Do, Hoa
name: Le, Thi
name: Hua, Phan
name: Vo, Hung Van
name: Nguyen, Diep
name: Nguyen, Dang
name: Uchida, Yuko
name: Saito, Reiko
name: Saito, Takehiko
Publisher: name: Figshare
Year: 2016
Subjects: subject: Medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Science Policy
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: 60506 Virology

description: Farm and sample information sheets used in the interviews with farm owners in this study. (DOC 107Â kb)
Waldpolitik 2020. Stand der Massnahmenumsetzung und der Zielerreichung Ende 2015
Creators: name: Wilkes-Allemann, Jerylee
name: Zabel Von Felten, Astrid Mirjam
name: Lieberherr, Eva
Publisher: name: Schweizerischer Forstverein
Year: 2018

description: Im Auftrag des Bundesamts für Umwelt (BAFU) wurde im Jahr 2016 eine Zwischenevaluation der ersten Etappe (2012–2015) der Waldpolitik 2020 durchgeführt. Hauptziel war es, den Stand der Massnahmenumsetzung und der Zielerreichung aufzuarbeiten, um Folgerungen für die zweite Etappe ziehen zu können. Dieser Beitrag fasst die wichtigsten Ergebnisse zusammen.
Additional file 4: of Prognostic value of morphologic and morphometric analyses in IgA nephropathy biopsies
Creators: name: Viola, Patrizia
name: Centurione, Lucia
name: Felaco, Paolo
name: Lattanzio, Giuseppe
name: DâAntuono, Tommaso
name: Liberatore, Marcella
name: Pietro, Roberta Di
name: Ranelletti, Franco
name: Bonomini, Mario
name: Aiello, Francesca
Publisher: name: Figshare
Year: 2016
Subjects: subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Molecular Biology
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics
subject: Developmental Biology
subject: Marine Biology
subject: Cancer
subject: Inorganic Chemistry
subject: FOS: Chemical sciences

description: Reading keys for database 2. (DOC 26Â kb)
Additional file 1: of The Munich-Evaluation-of-Mentoring-Questionnaire (MEMeQ) – a novel instrument for evaluating protégés’ satisfaction with mentoring relationships in medical education
Creators: name: Schäfer, Matthias
name: Pander, Tanja
name: Pinilla, Severin
name: Fischer, Martin
name: Borch, Philip Von Der
name: Dimitriadis, Konstantinos
Publisher: name: Figshare
Year: 2015
Subjects: subject: Biophysics
subject: Medicine
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Science Policy

description: The Munich-Evaluation-of-Mentoring-Questionnaire (MEMeQ). (DOCX 124 kb)
Hip-abductor fatigue influences sagittal plane ankle kinematics and shank muscle activity during a single-leg forward jump
Creators: name: Gafner, Simone C.
name: Hoevel, Veronika
name: Punt, Ilona M.
name: Schmid, Stefan
name: Armand, Stephane
name: Allet, Lara
Publisher: name: Elsevier
Year: 2018

description: Diminished hip abductor strength decreases postural control which is a parameter that is associated with an increased risk of ankle sprains. In our study we evaluated the influence of hip abductor fatigue on sagittal plane ankle kinematics and shank muscle activity during a single-leg forward jump. Sagittal ankle plane kinematics are important in ankle sprains but less studied than frontal plane kinematics. Therefore, we evaluated differences in sagittal ankle kinematics (12-camera motion capture system) and lower limb surface electromyographic muscle activity in 20 healthy, recreationally active adults (9 females, 11 males, mean age 30.3 SD 4.0 years, mean BMI 23.6 SD 2.8 kg/m2) before and after a hip abductor fatigue protocol (200-ms prior, at and in the 250-ms following initial contact (IC)). After fatigue, the maximal ankle plantar-flexion angle decreased prior to IC (median 3.8° [interquartile range 0.1, 7.2], p = 0.014), at IC (4.1° [−0.3, 5.0], p = 0.027) and post IC (4.1° [−1.3, 5.0] p = 0.036). Gastrocnemius activity onset was delayed (−28.0 ms [−44.0, 0.0], p < 0.01). Average activity of the tibialis anterior increased prior to IC (pre-fatigue 19.32% [14.89, 33.45], post-fatigue 28.95% [18.49, 34.81], p < 0.05). Hip-abductor fatigue influenced sagittal ankle kinematics and shank muscle activity during single-leg landings.
o-Vanillin binds covalently to MAL/TIRAP Lys-210 but independently inhibits TLR2
Creators: name: Rahaman, Md. Habibur
name: Thygesen, Sara J.
name: Maxwell, Michael J.
name: Kim, Hyoyoung
name: Mudai, Prerna
name: Nanson, Jeffrey D.
name: Jia, Xinying
name: Vajjhala, Parimala R.
name: Hedger, Andrew
name: Vetter, Irina
name: Haselhorst, Thomas
name: Robertson, Avril A. B.
name: Dymock, Brian
name: Ve, Thomas
name: Mobli, Mehdi
name: Stacey, Katryn J.
name: Kobe, Bostjan
Publisher: name: Taylor & Francis
Year: 2024
Subjects: subject: Biophysics
subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Molecular Biology
subject: Pharmacology
subject: Chemical Sciences not elsewhere classified
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Cancer
subject: Infectious Diseases
subject: FOS: Health sciences

description: Toll-like receptor (TLR) innate immunity signalling protects against pathogens, but excessive or prolonged signalling contributes to a range of inflammatory conditions. Structural information on the TLR cytoplasmic TIR (Toll/interleukin-1 receptor) domains and the downstream adaptor proteins can help us develop inhibitors targeting this pathway. The small molecule o-vanillin has previously been reported as an inhibitor of TLR2 signalling. To study its mechanism of action, we tested its binding to the TIR domain of the TLR adaptor MAL/TIRAP (MALTIR). We show that o-vanillin binds to MALTIR and inhibits its higher-order assembly in vitro. Using NMR approaches, we show that o-vanillin forms a covalent bond with lysine 210 of MAL. We confirm in mouse and human cells that o-vanillin inhibits TLR2 but not TLR4 signalling, independently of MAL, suggesting it may covalently modify TLR2 signalling complexes directly. Reactive aldehyde-containing small molecules such as o-vanillin may target multiple proteins in the cell.
Additional file 4: Table S4. of Changes in malaria burden and transmission in sentinel sites after the roll-out of long-lasting insecticidal nets in Papua New Guinea
Creators: name: Hetzel, Manuel
name: Reimer, Lisa
name: Gibson Gideon
name: Gussy Koimbu
name: CĂŠline Barnadas
name: Makita, Leo
name: Siba, Peter
name: Mueller, Ivo
Publisher: name: Figshare
Year: 2016
Subjects: subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: Cancer
subject: Computational Biology

description: Morbidity indicators in the general population before and after LLIN distribution. (DOCX 19Â kb)
Hylebainosoma Verhoeff 1899
Creators: name: Tajovský, Karel
name: Mock, Andrej
name: Papáč, Vladimír
Publisher: name: Zenodo
Year: 2014
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Diplopoda
Chordeumatida
Haaseidae
Hylebainosoma

description: Genus Hylebainosoma Verhoeff, 1899
syn. Romanosoma Ceuca, 1967
description: Published as part of Tajovský, Karel, Mock, Andrej & Papáč, Vladimír, 2014, The genus Hylebainosoma Verhoeff, 1899 (Diplopoda, Chordeumatida, Haaseidae): Redescription of Hylebainosoma tatranum, description of a new troglobiont species and notes to the Hylebainosoma – Romanosoma species group, pp. 501-523 in Zootaxa 3764 (5) on page 518, DOI: 10.11646/zootaxa.3764.5.1, http://zenodo.org/record/231022
Discovery of novel and potent CDK8 inhibitors for the treatment of acute myeloid leukaemia
Creators: name: Chen, Zhuoying
name: Wang, Quan
name: Yan, Yao Yao
name: Jin, Dalong
name: Wang, Yumeng
name: Zhang, Xing Xing
name: Liu, Xin Hua
Publisher: name: Taylor & Francis
Year: 2024
Subjects: Biochemistry
Cell Biology
Pharmacology
Environmental Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Cancer
Hematology

description: It has been reported that CDK8 plays a key role in acute myeloid leukaemia. Here, a total of 40 compounds were rational designed and synthesised based on the previous SAR. Among them, compound 12 (3-(3-(furan-3-yl)-1H-pyrrolo[2,3-b]pyridin-5-yl)benzamide) showed the most potent inhibiting activity against CDK8 with an IC50 value of 39.2 ± 6.3 nM and anti AML cell proliferation activity (molm-13 GC50 = 0.02 ± 0.01 μM, MV4-11 GC50 = 0.03 ± 0.01 μM). Mechanistic studies revealed that this compound 12 could inhibit the phosphorylation of STAT-1 and STAT-5. Importantly, compound 12 showed relative good bioavailability (F = 38.80%) and low toxicity in vivo. This study has great significance for the discovery of more efficient CDK8 inhibitors and the development of drugs for treating AML in the future.
Additional file 3: Table S3. of Changes in malaria burden and transmission in sentinel sites after the roll-out of long-lasting insecticidal nets in Papua New Guinea
Creators: name: Hetzel, Manuel
name: Reimer, Lisa
name: Gibson Gideon
name: Gussy Koimbu
name: CĂŠline Barnadas
name: Makita, Leo
name: Siba, Peter
name: Mueller, Ivo
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biochemistry
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics
subject: Cancer
subject: Computational Biology

description: Morbidity indicators in two sentinel health facilities before and after LLIN distribution. (DOCX 15Â kb)
Discovery of selective ACAT2 antagonist via a combination strategy based on deep docking, pharmacophore modelling, and molecular dynamics simulation
Creators: name: Liu, Yanfeng
name: Ding, Feng
name: Deng, Liangying
name: Zhang, Shuran
name: Wu, Lixing
name: Tong, Huangjin
Publisher: name: Taylor & Francis
Year: 2024
Subjects: subject: Biochemistry
subject: Physiology
subject: FOS: Biological sciences
subject: Pharmacology
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Information Systems not elsewhere classified
subject: Cancer

description: Acyl-CoA: cholesterol acyltransferase (ACAT), a pivotal enzyme in the absorption and metabolism of cholesterol, is primarily responsible for intracellular esterification. ACAT inhibition is expected to diminish plasma lipid levels by impeding intestinal cholesterol absorption, thereby preventing the progression of atherosclerotic lesions. A previous study shows that selective inhibition of ACAT2 significantly mitigated hypercholesterolaemia and atherosclerosis in mouse models. Therefore, the need for ACAT2 selective inhibitors becomes particularly urgent. In this study, we established a multilayer virtual screening workflow and subjected biologically evaluated representative compounds to enzyme inhibitory assays. The experimental results indicated that the two compounds, STL565001 (inhibition rate at 25 μM: 75.7 ± 27.8%, selectivity = 6) and STL528213 (inhibition rate at 25 μM: 87.8 ± 12.4%, selectivity = 13), demonstrated robust activity against ACAT2, displaying greater selectivity for ACAT2 than for ACAT1. The molecular mechanisms governing the inhibitory activities of the selected compounds were systematically elucidated using computational approaches. In addition, hotspot residues in ACAT2 that are crucial for ligand binding were successfully identified. In summary, we devised a multilayer screening scheme to expeditiously and efficiently identify compounds with enzyme inhibitory activity, offering novel scaffolds for subsequent drug design centred on ACAT2 targets.
Additional file 2: Figure S1. of Distribution of the DNA transposon family, Pokey in the Daphnia pulex species complex
Creators: name: Eagle, Shannon
name: Crease, Teresa
Publisher: name: Figshare
Year: 2016
Subjects: subject: Microbiology
subject: FOS: Biological sciences
subject: Genetics
subject: Molecular Biology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified

description: Correlation between 18S and 28S rRNA gene number D. pulex lineages. Figure S2. Correlation between 28S rRNA gene and rPokey family number in D. pulex lineages. Figure S3. Repeat landscape for mPok from Daphnia arenata isolate AR1.1. (PDF 34Â kb)
MOESM1 of Structural and functional characterization of a highly stable endo-β-1,4-xylanase from Fusarium oxysporum and its development as an efficient immobilized biocatalyst
Creators: name: Gómez, Sara
name: Payne, Asia
name: Savko, Martin
name: Fox, Gavin
name: Shepard, William
name: Fernandez, Francisco
name: M. Cristina Vega
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biophysics
subject: Biochemistry
subject: Space Science
subject: Medicine
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences

description: Additional file 1. Additional Fig. 1. Schematic representation of Xyl2 topology. Additional Fig. 2. Docking of a xylose hexaoligosaccharide on Xyl2 (pH 5). Additional Fig. 3. Schematic representation of the rationale for random enzyme immobilization via the carrier or carrier-free approaches. Negative correlation between Xyl2 activity yield and functionalization degree in high and low agarose supports. Additional Table 1. Guiding values for binding capacities of commercial agarose beads employed for Xyl2 immobilization.
Additional file 3: of Health literacy and refugees’ experiences of the health examination for asylum seekers – a Swedish cross-sectional study
Creators: name: Wångdahl, Josefin
name: Lytsy, Per
name: Mårtensson, Lena
name: Westerling, Ragnar
Publisher: name: Figshare
Year: 2015
Subjects: subject: Sociology
subject: FOS: Sociology
subject: Developmental Biology
subject: Science Policy

description: Questions about and dependent variables in the health examination with response categories. (DOCX 13 kb)
Hylebainosoma tatranum Verhoeff 1899
Creators: name: Tajovský, Karel
name: Mock, Andrej
name: Papáč, Vladimír
Publisher: name: Zenodo
Year: 2014
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Diplopoda
Chordeumatida
Haaseidae
Hylebainosoma
Hylebainosoma tatranum

description: Hylebainosoma tatranum Verhoeff, 1899
Hylebainosoma tatranum Verhoeff, 1899: 136 –137, Figs. 3–6
Hylebainosoma tatranum var. Dudichi Verhoeff, 1941: 232 –234, Figs. 1–4 Hylebainosoma tatranum jósvaense Loksa, 1962: 158 –159, Figs. 9–11
Hylebainosoma nontronensis Mauriès & Kime 1999): 371–373, Fig. 2
Hylebainososma gulickai n. sp. this contribution
Hylebainosoma cavernicola (Ceuca, 1967) n. comb.
syn. Romanosoma cavernicola Ceuca, 1967: 107 –109, Figs. I–III Hylebainosoma oltenica (Ceuca, 1967) n. comb.
syn. Romanosoma oltenica Ceuca, 1967: 109 –111, Figs. IV–VI
Hylebainosoma odici (Ceuca, 1979) n. comb.
syn. Romanosoma odici Ceuca, 1979: 337 –339, Figs. 1–3
[Romanosoma (?) birtei Ceuca, 1967: 111 –113; nom. nud.]
description: Published as part of Tajovský, Karel, Mock, Andrej & Papáč, Vladimír, 2014, The genus Hylebainosoma Verhoeff, 1899 (Diplopoda, Chordeumatida, Haaseidae): Redescription of Hylebainosoma tatranum, description of a new troglobiont species and notes to the Hylebainosoma – Romanosoma species group, pp. 501-523 in Zootaxa 3764 (5) on pages 518-519, DOI: 10.11646/zootaxa.3764.5.1, http://zenodo.org/record/231022
Additional file 1: Figure S1. of Voluntary resistance wheel exercise from mid-life prevents sarcopenia and increases markers of mitochondrial function and autophagy in muscles of old male and female C57BL/6J mice
Creators: name: White, Zoe
name: Terrill, Jessica
name: White, Robert
name: McMahon, Christopher
name: Sheard, Phillip
name: Grounds, Miranda
name: Shavlakadze, Tea
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Neuroscience
subject: Physiology
subject: Pharmacology
subject: Immunology
subject: FOS: Clinical medicine
subject: 111714 Mental Health
subject: FOS: Health sciences
subject: Computational Biology

description: Murf1 (A) and Atrogin-1 (B) mRNA in quadriceps muscles of 15-month SED, 23-month SED, and 23-month RWE mice, of both sexes. Gene expression in the quadriceps muscles was normalized to the geometric mean of Hprt and Ppia expression values. Data were analyzed by ANOVA, using age and sex, and sex and activity as variables. Data are mean ± SEM. Asterisk (*) denotes significance at *P
Novel anti-neuroinflammatory pyranone-carbamate derivatives as selective butyrylcholinesterase inhibitors for treating Alzheimer’s disease
Creators: name: Yu, Chuanyu
name: Liu, Xueyan
name: Ma, Bingxiang
name: Xu, Jiexin
name: Chen, Yiquan
name: Dai, Chaoxian
name: Peng, Huaping
name: Zha, Daijun
Publisher: name: Taylor & Francis
Year: 2024
Subjects: Biochemistry
Neuroscience
Pharmacology
Biotechnology
Chemical Sciences not elsewhere classified
Cancer
Virology

description: Butyrylcholinesterase (BuChE) and neuroinflammation have recently emerged as promising therapeutic directions for Alzheimer’s disease (AD). Herein, we synthesised 19 novel pyranone-carbamate derivatives and evaluated their activities against cholinesterases and neuroinflammation. The optimal compound 7p exhibited balanced BuChE inhibitory activity (eqBuChE IC50 = 4.68 nM; huBuChE IC50 = 9.12 nM) and anti-neuroinflammatory activity (NO inhibition = 28.82% at 10 μM, comparable to hydrocortisone). Enzyme kinetic and docking studies confirmed compound 7p was a mix-type BuChE inhibitor. Additionally, compound 7p displayed favourable drug-likeness properties in silico prediction, and exhibited high BBB permeability in the PAMPA-BBB assay. Compound 7p had good safety in vivo as verified by an acute toxicity assay (LD50 > 1000 mg/kg). Most importantly, compound 7p effectively mitigated cognitive and memory impairments in the scopolamine-induced mouse model, showing comparable effects to Rivastigmine. Therefore, we envisioned that compound 7p could serve as a promising lead compound for treating AD.