title: ÐÐ°Ð²Ð¸ÑÐ¸Ð¼Ð¾ÑÑÑ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÑÐ¸ÑÑÐµÐ¼Ñ ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ II ÑÐ¸Ð¿Ð° Esp1396I Ð¾Ñ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð° ÑÐµÐ³ÑÐ»ÑÑÐ¾ÑÐ½Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° C.Esp1396I Ð² ÐºÐ»ÐµÑÐºÐµ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Dependence of Esp1396I restriction-modification II type system protection efficiency on amount of regulatory protein C.Esp1396I
Creators: name: ÐÐ¾ÑÐ¾Ð²ÑÐºÐ°Ñ, ÐÐºÑÐ°Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÑÐ¸ÑÑÐµÐ¼Ð° ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ II ÑÐ¸Ð¿Ð°
Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³ Î»
Esp1396I
ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð°Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ
ÐÐÐ-ÑÐ²ÑÐ·ÑÐ²Ð°ÑÑÐ¸Ð¹ Ð±ÐµÐ»Ð¾Ðº
ÑÐµÐ³ÑÐ»ÑÑÐ¸Ñ ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸Ð¸
type II restriction-modification system
bacteriophage Î»
fluorescence microscopy
DNA-binding protein
transcription regulation

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«ÐÐ°Ð²Ð¸ÑÐ¸Ð¼Ð¾ÑÑÑ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÑÐ¸ÑÑÐµÐ¼Ñ ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ II ÑÐ¸Ð¿Ð° Esp1396I Ð¾Ñ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð° ÑÐµÐ³ÑÐ»ÑÑÐ¾ÑÐ½Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° C.Esp1396I Ð² ÐºÐ»ÐµÑÐºÐµÂ».Ð¡Ð¸ÑÑÐµÐ¼Ñ ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ â ÑÑÐ¾ Ð·Ð°ÑÐ¸ÑÐ½ÑÐµ ÑÐ¸ÑÑÐµÐ¼Ñ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ Ð¸ Ð°ÑÑÐµÐ¹, Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÑÑÐ¸Ðµ Ð·Ð°ÑÐ¸ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð¾Ñ Ð¸Ð½Ð²Ð°Ð·Ð¸Ð²Ð½Ð¾Ð¹ ÐÐÐ. Ð ÑÐ¾ÑÑÐ°Ð²Ðµ ÑÐ¸ÑÑÐµÐ¼ ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Ð·Ð°ÑÐ¸ÑÐ° Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐµÑÑÑ Ð·Ð° ÑÑÑÑ ÑÐµÑÐ¼ÐµÐ½ÑÐ¾Ð², Ð¾Ð±Ð»Ð°Ð´Ð°ÑÑÐ¸Ðµ Ð´Ð²ÑÐ¼Ñ Ð²Ð¸Ð´Ð°Ð¼Ð¸ ÐºÐ°ÑÐ°Ð»Ð¸ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸: ÑÐ½Ð´Ð¾Ð½ÑÐºÐ»ÐµÐ°Ð·Ð½Ð°Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ, Ð±Ð»Ð°Ð³Ð¾Ð´Ð°ÑÑ ÐºÐ¾ÑÐ¾ÑÐ¾Ð¹ Ð¿ÑÐ¾Ð¸ÑÑÐ¾Ð´Ð¸Ñ ÑÐ°ÑÑÐµÐ¿Ð»ÐµÐ½Ð¸Ðµ ÑÑÐ¶ÐµÑÐ¾Ð´Ð½Ð¾Ð¹ ÐÐÐ Ð² ÑÐ°Ð¹ÑÐ°Ñ ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸, Ð¸ Ð¼ÐµÑÐ¸Ð»ÑÑÐ°Ð½ÑÑÐµÑÐ°Ð·Ð½Ð°Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ, Ð¾Ð±ÐµÑÐ¿ÐµÑÐµÐ½Ð¸Ðµ ÐºÐ¾ÑÐ¾ÑÐ¾Ð¹ Ð¿ÑÐ¸Ð²Ð¾Ð´Ð¸Ñ Ðº Ð·Ð°ÑÐ¸ÑÐµ ÐÐÐ-ÐºÐ»ÐµÑÐºÐ¸ ÑÐ¾Ð·ÑÐ¸Ð½Ð° Ð¾Ñ ÑÐ°ÑÑÐµÐ¿Ð»ÐµÐ½Ð¸Ñ Ð² Ð´Ð°Ð½Ð½ÑÑ ÑÐ°Ð¹ÑÐ°Ñ. Ð­ÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð·Ð°ÑÐ¸ÑÑ Ð·Ð°Ð²Ð¸ÑÐ¸Ñ Ð¾Ñ Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð¹ Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐ¸ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð±ÐµÐ»ÐºÐ¾Ð² ÑÐ¸ÑÑÐµÐ¼Ñ ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸, Ð¿Ð¾ÑÑÐ¾Ð¼Ñ Ð²Ð°Ð¶Ð½Ð° ÑÐµÐ³ÑÐ»ÑÑÐ¸Ñ Ð¸Ñ ÑÐ°Ð±Ð¾ÑÑ.ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¾ÑÐµÐ½ÐºÐµ Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾ÑÑÐ¸ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÑÐ¸ÑÑÐµÐ¼Ñ ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ II ÑÐ¸Ð¿Ð° Esp1396I Ð¾Ñ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð° ÑÐµÐ³ÑÐ»ÑÑÐ¾ÑÐ½Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° C.Esp1396I Ð² ÐºÐ»ÐµÑÐºÐµ. Ð¡ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð°Ð½Ð°Ð»Ð¸Ð·Ð° ÑÑÐ¾Ð²Ð½Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸ (Ð½Ð° ÑÑÐ¾Ð²Ð½Ðµ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ ÐºÑÐ»ÑÑÑÑ Ð¸ Ð½Ð° ÑÑÐ¾Ð²Ð½Ðµ Ð¾ÑÐ´ÐµÐ»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº) Ð¸ Ð¾Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÐ¸ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ ÐºÑÐ»ÑÑÑÑ Ð±ÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ðµ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð° Ð³Ð¸Ð±ÑÐ¸Ð´Ð½Ð¾Ð³Ð¾ ÑÐµÐ³ÑÐ»ÑÑÐ¾ÑÐ½Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° C.Esp1396I, ÑÐ»Ð¸ÑÐ¾Ð³Ð¾ Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½ÑÐ¼ Ð±ÐµÐ»ÐºÐ¾Ð¼ mNeonGreen, Ð¿ÑÐ¸Ð²Ð¾Ð´Ð¸Ñ Ðº Ð½Ð°ÑÑÑÐµÐ½Ð¸Ñ Ð±Ð°Ð»Ð°Ð½ÑÐ° Ð±ÐµÐ»ÐºÐ¾Ð² ÑÐ¸ÑÑÐµÐ¼Ñ ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Esp1396I, ÑÑÐ¾ Ð¿Ð¾ÑÐ¾Ð¶Ð´Ð°ÐµÑ Ð·Ð½Ð°ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ðµ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð·Ð°ÑÐ¸ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ Ð¾Ñ Ð²Ð¸ÑÑÑÐ°.
description: The subject of the graduate qualification work is âDependence of Esp1396I restriction-modification II type system protection efficiency on amount of regulatory protein C.Esp1396Iâ.Restriction-modification systems are protective systems of bacteria and archaea that prevent invasive DNA spreading in cells. As part of restriction-modification systems, protection is carried out by enzymes having two types of catalytic activity: endonuclease activity, due to which foreign DNA is cleaved at restriction sites, and methyltransferase activity, the effort of which leads to the protection of the host DNA cell from cleavage at these sites. The effectiveness of protection depends on the temporal dynamics of the restriction-modification system proteins expression; therefore, regulation of their work is too important.This work is devoted to the evaluation of the dependence of the efficiency of the restriction-modification system type II Esp1396I on the amount of regulatory protein C.Esp1396I in cells. Using methods of analysis of the level of fluorescence (at the level of cell cultures and at the level of individual cells) and the optical density of cell cultures, it was shown that an increase in the amount of hybrid regulatory protein C.Esp1396I fused with the fluorescent protein mNeonGreen leads to a violation of the restriction-modification system protein balance, which generates a significant change in the effectiveness of protecting bacteria from the bacteriophage.