Supplementary materials
Creators: name: Zhao, Meisheng
Publisher: name: figshare
Year: 2024
Subjects: classificationCode: 310706
subject: Virology
classificationCode: 310805
subject: Plant pathology
classificationCode: 310510
subject: Molecular evolution

description: Fig. S1 SlGeBP has sequence-non-specific DNA binding activity. Fig. S2 No interaction between SlGeBP and viral protein (V1, V2, C1, C2, C3 and C4) encoded by TbCSV. Fig. S3 Phylogenetic analysis of GeBP protein family. The phylogenetic tree of GeBP protein family in several species (S. lycopersicum, S. tuberosum, N. benthamiana, C. annuum, A. thaliana and T. aestivum) was analyzed by MEGA X software by the Maximum-Likelihood method. The bootstrap consensus tree inferred from 1000 replicates is taken to represent the evolutionary history of the taxa analyzed. Fig. S4 NbGeBP protein has self-interaction. BiFC analysis of NbGeBP protein self-interaction in N. benthamiana leaves. The RFP-H2B is nuclear localization marker. Co-expression of YN-NbGeBP and YC or YN and YC-NbGeBP were used as negative controls. Confocal imaging was performed at 48 hpai. Scale bars, 50 μm. These experiments were performed with three independent biological replicates with similar results. Fig. S5 SlGeBP is not required for TbCSV/TbCSB infection in tomato. A, Symptoms of tomato plants inoculated with PVX-GUS, or PVX-SlGeBP at 19 dpi. B, RT-qPCR analysis SlGeBP mRNA levels in the systemic leaves of tomato plants shown in a. Data are the mean of three independent biological replicates. Error bars represent SEM. An asterisk indicates a statistically significant difference according to unpaired Student’s t test (two-tailed), * p &lt; 0.05. C, βC1 analysis by PCR in the systemic leaves of tomato plants shown in a inoculated with TbCSV+B at 12 dpi. D, qPCR analysis of TbCSV and TbCSB DNA accumulation in the samples shown in c. Data are the mean of eight independent biological replicates. Error bars represent SEM. The ns indicates no significant difference according to unpaired Student’s t test (two-tailed). Fig. S6 AtGeBP is required for TbCSV/TbCSB infection in Arabidopsis. A, Locations of T-DNA inserts in AT5G28040.1 gene. Gray boxes represent exons, and horizontal lines represent introns or untranslated regions (UTR). T-DNA inserts (arrowheads) are not drawn to scale. B, Molecular characterization of AtGeBP T-DNA insertion alleles in Arabidopsis plants. C, Symptoms of Arabidopsis plants inoculated with TbCSV+B at 7 dpi. D, qPCR analysis of TbCSV and TbCSB DNA accumulation in the systemic leaves of Arabidopsis plants shown in c. Data are the mean of four independent biological replicates. Error bars represent SEM. An asterisk indicates a statistically significant difference according to unpaired Student’s t test (two-tailed), * p &lt; 0.05, ** p &lt; 0.01. Fig. S7 Phylogenetic analysis of βC1 proteins encoded by betasatellites of monopartite begomoviruses. Total 134 βC1 protein sequences were get from a reference sequence database at the NCBI and aligned by the ClustalW program in BioEdit v. 7.2.6 with default parameters to produce a FASTA file, which further analyzed by MEGA X software by the Maximum-Likelihood method to generate a phylogenetic tree. The betasatellites initially discovered in Solanaceae plants were marked with red fonts. The detailed information of 134 βC1 proteins is available in Table S4. Fig. S8 The tissue-specific expression of SlGeBP (Solyc05g051330) in Tomato eFP Browser. Fig. S9 The tissue-specific expression of NbGeBP (Niben101Scf10342g01018.1) in Nb Browser. Fig. S10 NbGeBP (Niben101Scf10342g01018.1) is different from the other 11 proteins in N. benthamiana GeBP family. A, The amino acid sequences identity of N. benthamiana GeBP family proteins. B, Phylogenetic tree was constructed by the Maximum-Likelihood method using the amino acid sequences from N. benthamiana GeBP family proteins. Table S1 Primers used in this study. Table S2 The candidate interacting proteins of βC1 identified in tomato cDNA library through Y2H screening. Table S3 The host range of TbCSV, TbCSV/TbCSB, TYLCCNV/TYLCCNB, and MaYVV/MaYVB. Table S4 The detailed information of 134 βC1 proteins.