title: ÐÐ½Ð¾Ð³Ð¾ÐºÐ°Ð½Ð°Ð»ÑÐ½Ð°Ñ ÑÐ¸ÑÑÐµÐ¼Ð° Ð¿Ð¾Ð´Ð³Ð¾ÑÐ¾Ð²ÐºÐ¸ Ð¼Ð¸ÐºÑÐ¾ÑÐ»ÑÐ¸Ð´Ð½ÑÑ ÑÐ¸Ð¿Ð¾Ð² Ð´Ð»Ñ ÐÐ¦Ð -Ð°Ð½Ð°Ð»Ð¸Ð·Ð°
title: Multichannel microfluidic chip preparation system for PCR analysis
Creators: name: ÐÑÐ»ÑÐ½Ð¸ÑÐ°, ÐÐ½ÑÐ¾Ð½
name: ÐÑÐ¸ÐºÐ¾Ð²Ð°, ÐÐ°Ð´ÐµÐ¶Ð´Ð°
name: ÐÐ²ÑÑÑÐ°Ð¿Ð¾Ð², ÐÐ½Ð°ÑÐ¾Ð»Ð¸Ð¹
name: "Ð¡Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ðµ Ð¼Ð°ÑÐ¸Ð½Ð¾ÑÑÑÐ¾ÐµÐ½Ð¸Ðµ: Ð½Ð°ÑÐºÐ° Ð¸ Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ðµ", Ð¼ÐµÐ¶Ð´ÑÐ½Ð°ÑÐ¾Ð´Ð½Ð°Ñ Ð½Ð°ÑÑÐ½Ð°Ñ ÐºÐ¾Ð½ÑÐµÑÐµÐ½ÑÐ¸Ñ (13; 2024; Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³)
Publisher: name: ÐÐÐÐÐ¢ÐÐ¥-ÐÐ ÐÐ¡Ð¡
Year: 2024
Subjects: ÐÑÐ¸Ð±Ð¾ÑÐ¾ÑÑÑÐ¾ÐµÐ½Ð¸Ðµ
ÐÐ¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð°Ñ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ñ
Ð¢ÐµÑÐ¼Ð¾Ð¿Ð»Ð°ÑÑÑ
Ð¼Ð½Ð¾Ð³Ð¾ÐºÐ°Ð½Ð°Ð»ÑÐ½Ð¾Ðµ Ð¼Ð¸ÐºÑÐ¾ÑÐ»ÑÐ¸Ð´Ð½Ð¾Ðµ ÑÑÑÑÐ¾Ð¹ÑÑÐ²Ð¾
ÑÐ°ÑÑÐ²Ð¾ÑÐµÐ½Ð¸Ðµ
Ð´Ð¸ÑÑÑÐ·Ð¸Ñ
Ð°ÐºÑÐ¸Ð²Ð½Ð¾Ðµ Ð¿ÐµÑÐµÐ¼ÐµÑÐ¸Ð²Ð°Ð½Ð¸Ðµ
Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð½Ð°Ñ ÑÐµÐ¿Ð½Ð°Ñ ÑÐµÐ°ÐºÑÐ¸Ñ Ð² ÑÐµÐ°Ð»ÑÐ½Ð¾Ð¼ Ð²ÑÐµÐ¼ÐµÐ½Ð¸
Ð·Ð°Ð¿Ð¾Ð»Ð½ÐµÐ½Ð¸Ðµ ÑÐµÐ°ÐºÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÐºÐ°Ð¼ÐµÑÑ
multichannel microfluidic device
dissolution
diffusion
active mixing
real-time polymerase chain reaction
filling of the reaction chamber

description: Ð¡Ð¾Ð·Ð´Ð°Ð½Ð¸Ðµ Ð¼Ð¸ÐºÑÐ¾ÑÐ»ÑÐ¸Ð´Ð½ÑÑ ÑÐ¸Ð¿Ð¾Ð² Ñ ÑÐµÐ°Ð³ÐµÐ½ÑÐ°Ð¼Ð¸, Ð²Ð½ÐµÐ´ÑÐµÐ½Ð½ÑÐ¼Ð¸ Ð² ÑÐµÐ°ÐºÑÐ¸Ð¾Ð½Ð½ÑÑ ÐºÐ°Ð¼ÐµÑÑ, Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÐµÑ ÑÑÑÐµÑÑÐ²ÐµÐ½Ð½Ð¾ ÑÐ¿ÑÐ¾ÑÑÐ¸ÑÑ Ð¿Ð¾Ð´Ð³Ð¾ÑÐ¾Ð²Ð¸ÑÐµÐ»ÑÐ½ÑÐµ Ð¿ÑÐ¾ÑÐµÐ´ÑÑÑ Ð¸ ÑÐ¾ÐºÑÐ°ÑÐ¸ÑÑ Ð²ÑÐµÐ¼Ñ Ð¿ÑÐ¸ Ð°Ð½Ð°Ð»Ð¸Ð·Ðµ Ð¿ÑÐ¾Ð±Ñ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ ÐÐ¦Ð -Ð Ð. Ð ÑÐ°Ð±Ð¾ÑÐµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ñ Ð¸ Ð¾Ð±ÑÑÐ¶Ð´ÐµÐ½Ñ Ð½ÐµÐºÐ¾ÑÐ¾ÑÑÐµ ÐºÐ»ÑÑÐµÐ²ÑÐµ Ð¿ÑÐ¾ÑÐµÑÑÑ, Ð¿ÑÐ¾ÑÐµÐºÐ°ÑÑÐ¸Ðµ Ð¿ÑÐ¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ð¸ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ ÐÐ¦Ð -Ð Ð Ð² Ð¼Ð¸ÐºÑÐ¾ÑÐ»ÑÐ¸Ð´Ð½ÑÑ ÑÐ¸Ð¿Ð°Ñ Ð¸Ð· Ð¿Ð¾Ð»Ð¸ÐºÐ°ÑÐ±Ð¾Ð½Ð°ÑÐ° Ñ Ð»Ð¸Ð¾ÑÐ¸Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¼Ð¸ ÑÐµÐ°Ð³ÐµÐ½ÑÐ°Ð¼Ð¸. ÐÑÐµÐ½ÐºÐ° Ð´ÐµÑÐ¾ÑÐ¼Ð°ÑÐ¸Ð¸ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ½Ð¾Ð³Ð¾ ÑÐ¸Ð¿Ð° Ð²ÑÐ»ÐµÐ´ÑÑÐ²Ð¸Ðµ ÑÐµÐ¿Ð»Ð¾Ð²Ð¾Ð³Ð¾ ÑÐ°ÑÑÐ¸ÑÐµÐ½Ð¸Ñ Ð¿ÑÐ¸ ÑÐ¸ÐºÐ»Ð¸ÑÐµÑÐºÐ¸Ñ ÑÐµÐ¶Ð¸Ð¼Ð°Ñ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÑ Ð² Ð¿ÑÐµÐ´ÐµÐ»Ð°Ñ 60 â 95 Ð¾Ð¡ ÑÐ²Ð¸Ð´ÐµÑÐµÐ»ÑÑÑÐ²ÑÐµÑ Ð¾ Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼Ð¾ÑÑÐ¸ ÑÑÐ¸ÑÑÐ²Ð°ÑÑ ÑÑÐ¸ ÑÑÑÐµÐºÑÑ Ð¿ÑÐ¸ ÐºÐ¾Ð½ÑÑÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ ÑÐ¸Ð¿Ð¾Ð² Ð¸Ð· ÑÐµÑÐ¼Ð¾Ð¿Ð»Ð°ÑÑÐ¸ÑÐ½ÑÑ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ð¾Ð². ÐÑÐµÐ´Ð²Ð°ÑÐ¸ÑÐµÐ»ÑÐ½ÑÐ¹ ÑÐ°ÑÑÐµÑ ÑÑÐ»Ð¾Ð²Ð¸Ð¹ Ð·Ð°Ð³ÑÑÐ·ÐºÐ¸ Ð¿ÑÐ¾Ð±Ñ Ð¿Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÐµÑ, ÑÑÐ¾ Ð¿Ð¾Ð´Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ðµ ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÑ Ð² Ð¸Ð½ÑÐµÑÐ²Ð°Ð»Ðµ Ð¾Ñ 50 Ð´Ð¾ 90 Ð¾Ð¡ Ð² ÑÐµÑÐµÐ½Ð¸Ðµ 5 Ð¼Ð¸Ð½ Ð¿ÐµÑÐµÐ´ Ð½Ð°ÑÐ°Ð»Ð¾Ð¼ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð¾Ð±ÐµÑÐ¿ÐµÑÐ¸Ð²Ð°ÐµÑ Ð¿ÑÐ°ÐºÑÐ¸ÑÐµÑÐºÐ¸ ÑÐ°Ð²Ð½Ð¾Ð¼ÐµÑÐ½Ð¾Ðµ ÑÐ°ÑÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð¿ÑÐ°Ð¹Ð¼ÐµÑÐ¾Ð² Ð¿Ð¾ Ð²ÑÐµÐ¹ ÑÐµÐ°ÐºÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÐºÐ°Ð¼ÐµÑÐµ Ð·Ð° ÑÑÐµÑ Ð´Ð¸ÑÑÑÐ·Ð¸Ð¾Ð½Ð½Ð¾Ð³Ð¾ Ð¿ÐµÑÐµÐ¼ÐµÑÐµÐ½Ð¸Ñ Ð¸ Ð´Ð¾Ð¿Ð¾Ð»Ð½Ð¸ÑÐµÐ»ÑÐ½ÑÐµ Ð¼ÐµÑÑ Ð¿Ð¾ Ð¸Ð½ÑÐµÐ½ÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ ÑÑÐ¾Ð³Ð¾ Ð¿ÑÐ¾ÑÐµÑÑÐ° Ð½Ðµ ÑÑÐµÐ±ÑÑÑÑÑ.
description: The creation of microfluidic chips with reagents embedded in the reaction chamber can significantly simplify preparatory procedures and reduce the time when analyzing a sample using RT-PCR. The work examines and discusses some of the key processes that occur during RT-PCR analysis in microfluidic polycarbonate chips with lyophilized reagents. An assessment of the deformation of a polymer chip due to thermal expansion under cyclic temperature changes in the range of 60â95 Â°C indicates the need to take these effects into account when designing chips from thermoplastic materials. A preliminary calculation of the sample loading conditions shows that maintaining the temperature in the range from 50 to 90 Â°C for 5 minutes before starting the analysis ensures an almost uniform distribution of primers throughout the reaction chamber due to diffusion movement and additional measures to intensify this process are not required.