title: ÐÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÐºÐ»ÐµÑÐ¾Ðº Ð² ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ð¾Ð²ÑÑ Ð³ÐµÐ»ÑÑ , Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ ÐºÐ°ÑÐ±Ð¾ÐºÑÐ¸Ð¼ÐµÑÐ¸Ð»ÑÐµÐ»Ð»ÑÐ»Ð¾Ð·Ð¾Ð¹
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Cell cultivation in collagen gels modified with carboxymethyl cellulose
Creators: name: ÐÐ¾Ð½ÑÐ¾Ð½, ÐÐ°Ð»ÐµÐ½ÑÐ¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÐÐ»ÐµÑÐºÐ° (Ð±Ð¸Ð¾Ð».)
ÐÐ¸Ð¾ÑÐµÑ Ð½Ð¾Ð»Ð¾Ð³Ð¸Ñ
Ð³Ð¸Ð´ÑÐ¾Ð³ÐµÐ»Ð¸
ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½
ÐºÐ°ÑÐ±Ð¾ÐºÑÐ¸Ð¼ÐµÑÐ¸Ð»ÑÐµÐ»Ð»ÑÐ»Ð¾Ð·Ð°
Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÐµ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸
ÑÑÐµÑ Ð¼ÐµÑÐ½Ð¾Ðµ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
hydrogels
collagen
carboxymethyl cellulose
mesenchymal stem cells
3D cell culture

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð½Ð° ÐºÐ»ÐµÑÐºÐ¸ Ð»Ð¸Ð½Ð¸Ð¸ fetMSC Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ð¾Ð²Ð¾Ð³Ð¾ Ð³ÐµÐ»Ñ ÐºÐ°ÑÐ±Ð¾ÐºÑÐ¸Ð¼ÐµÑÐ¸Ð»ÑÐµÐ»Ð»ÑÐ»Ð¾Ð·Ð¾Ð¹ (ÐÐÐ¦). ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ Ð±ÑÐ»Ð¸ ÑÐµÑÐµÐ½Ñ Ð² ÑÐ¾Ð´Ðµ ÑÐ°Ð±Ð¾ÑÑ: 1. Ð¡ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°ÑÑ Ð³ÐµÐ»Ð¸ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ð° Ñ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÐ¼ ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸ÐµÐ¼ ÐÐÐ¦. 2. ÐÑÐµÐ½Ð¸ÑÑ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Ð½Ð° ÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾ÑÑÑ Ð³ÐµÐ»ÐµÐ¹. 3. ÐÐ·ÑÑÐ¸ÑÑ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Ð½Ð° ÑÐ°ÐºÐ¸Ðµ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ¸ ÐºÐ»ÐµÑÐ¾Ðº, ÐºÐ°Ðº: Ð¶Ð¸Ð·Ð½ÐµÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ, Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ñ, ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ Ðº Ð°Ð´Ð³ÐµÐ·Ð¸Ð¸, Ð¿ÑÐ¾Ð»Ð¸ÑÐµÑÐ°ÑÐ¸Ð²Ð½Ð°Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ, ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ Ðº Ð¾ÑÑÐµÐ¾Ð³ÐµÐ½Ð½Ð¾Ð¹ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐµ Ð¸ ÑÐ¸Ð½ÑÐµÐ·Ñ ÑÐ¸Ð±ÑÐ¾Ð½ÐµÐºÑÐ¸Ð½Ð°. Ð ÑÐ°Ð±Ð¾ÑÐµ Ð±ÑÐ»Ð¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ ÑÐ°ÐºÐ¸Ðµ Ð¼ÐµÑÐ¾Ð´Ñ, ÐºÐ°Ðº ÑÐ²ÐµÑÐ¾Ð²Ð°Ñ, ÑÐ»ÐµÐºÑÑÐ¾Ð½Ð½Ð°Ñ Ð¸ ÐºÐ¾Ð½ÑÐ¾ÐºÐ°Ð»ÑÐ½Ð°Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð°Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ; ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÐ·; Ð¸Ð¼Ð¼ÑÐ½Ð¾ÑÐ¸ÑÐ¾ÑÐ¸Ð¼Ð¸Ñ. ÐÐ¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ ÐÐÐ¦ ÑÐ»ÑÑÑÐ°ÐµÑ Ð¼ÐµÑÐ°Ð½Ð¸ÑÐµÑÐºÐ¸Ðµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ð¾Ð²Ð¾Ð³Ð¾ Ð³ÐµÐ»Ñ Ð¸ Ð½Ðµ Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÐµÑ Ð½ÐµÐ³Ð°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð½Ð° Ð¶Ð¸Ð·Ð½ÐµÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð² Ð³ÐµÐ»ÑÑ, Ð° Ð¿ÑÐ¸ Ð´Ð»Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð¼ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ ÐºÐ»ÐµÑÐºÐ¸ Ð¿ÑÐ¾ÑÐ²Ð»ÑÑÑ Ð½Ð°Ð¸Ð±Ð¾Ð»ÑÑÑÑ Ð¿ÑÐ¾Ð»Ð¸ÑÐµÑÐ°ÑÐ¸Ð²Ð½ÑÑ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð² Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ Ð³ÐµÐ»ÑÑ. ÐÑÐ» ÑÐ´ÐµÐ»Ð°Ð½ Ð²ÑÐ²Ð¾Ð´ Ð¾ ÑÐ¾Ð¼, ÑÑÐ¾ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐµ ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ð¾Ð²ÑÐµ Ð¼Ð°ÑÑÐ¸ÑÑ Ð»ÑÑÑÐµ Ð¿Ð¾Ð´ÑÐ¾Ð´ÑÑ Ð´Ð»Ñ Ð´Ð¾Ð»Ð³Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð³Ð¾ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÐºÐ»ÐµÑÐ¾Ðº. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ Ð¼Ð°ÑÑÐ¸ÑÑ Ð¼Ð¾Ð³ÑÑ Ð±ÑÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ ÐºÐ°Ðº Ð¿Ð»Ð°ÑÑÐ¾ÑÐ¼Ñ Ð´Ð»Ñ ÑÐµÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð»ÐµÐºÐ°ÑÑÑÐ², ÐºÐ°Ðº ÑÐ¸ÑÑÐµÐ¼Ð° Ð´Ð»Ñ Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ ÐºÐ»ÐµÑÐ¾Ðº Ð¼ÐµÐ¶Ð´Ñ ÑÐ¾Ð±Ð¾Ð¹ Ð¸ Ñ Ð²Ð½ÐµÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐ¼ Ð¼Ð°ÑÑÐ¸ÐºÑÐ¾Ð¼ Ð¸ ÐºÐ°Ðº Ð½Ð¾ÑÐ¸ÑÐµÐ»Ñ Ð´Ð»Ñ ÑÑÐ°Ð½ÑÐ¿Ð»Ð°Ð½ÑÐ¸ÑÑÐµÐ¼ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð² ÑÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸Ð²Ð½Ð¾Ð¹ Ð¼ÐµÐ´Ð¸ÑÐ¸Ð½Ðµ.
description: The given work is devoted to the study of the effect of modification of collagen gel with carboxymethyl cellulose (CMC) on fetMSC cells. Tasks that were solved during the work: 1. Form gels based on collagen with different content of CMC. 2. Evaluate the effect of modification on gels stability. 3. To study the effect of modification on cell characteristics: viability, morphology, adhesion ability, proliferative activity, ability for osteogenic differentiation and fibronectin synthesis. Methods that were used in this work include light, electron and confocal fluorescence microscopy; electrophoresis; immunocytochemistry. It has been shown that CMC improves the mechanical properties of collagen gel and does not adversely affect cell viability in gels, and cells show the highest proliferative activity in modified gels during long-term cultivation. It was concluded that modified collagen matrices are better suited for long-term cell culture. The resulting matrices can be used as platforms for drug testing, as a system for studying of cells interaction with each other and with an extracellular matrix, and as a carrier for transplanted cells in regenerative medicine.