Additional file 1 of Contribution of tetrodotoxin-resistant persistent Na+ currents to the excitability of C-type dural afferent neurons in rats
Creators: name: Nakamura, Michiko
name: Jang, Il-Sung
Publisher: name: figshare
Year: 2022
Subjects: subject: Biophysics
subject: Biochemistry
subject: Cell Biology
subject: Neuroscience
subject: Physiology
subject: FOS: Biological sciences
subject: Evolutionary Biology

description: Additional file 1: Supplementary Fig. S1. Basal properties of TTX-RINaP and IRamp in adult male and female rats. A. Typical traces of TTX-R INaP(a) and IRamp (b) in the absence and presence of 0.1% DMSO (v/v). Similar results were obtained from five independent experiments. B. Scatter plots of the density of TTX-R INaP against membranecapacitance (Cm) obtained from DiI-positive neurons derived from young male (a, n = 71 neurons, same to Fig. 1D), adult male (b, n = 62 neurons), and adult female rats (c, n = 60neurons). The linear trend lines represent the best fit using a least-squaresfit (a; r = 0.65, b; r = 0.73, c; r = 0.76). C. The mean values of the density of TTX-R INaP in small-sized (a; n = 31 neurons for young male, n = 28 neurons for adult male, and n = 31 neurons for adult female rats) and medium-sized (b; n = 40 neurons for young male, n = 34 neurons for adult male, and n = 29 neurons for adult female rats) DiI-positive neurons. The columns and error bars represent the mean and SEM. n.s; not significant (unpaired t-test). D. Scatter plots of the density of TTX-R IRamp against membrane capacitance (Cm) obtained from DiI-positive neurons derived from young adult male (a, n = 152 neurons, same to Fig. 2Da), adult male (b, n = 62 neurons), and adult female rats (c,n = 60 neurons). The linear trend lines represent the best fit using aleast-squares fit (a; r = 0.47, b; r = 0.69, c; r= 0.76). E. The mean values of the density of TTX-R IRamp in small-sized (a; n = 60 neurons for young male, n = 28 neurons for adult male, and n = 31 neurons for adult female rats) and medium-sized (b; n = 92 neurons for young male, n = 34 neurons for adult male, and n = 29 neurons for adult female rats) DiI-positive neurons. The columns and errorbars represent the mean and SEM. n.s; not significant (unpaired t-test). Supplementary Fig. S2. Voltage dependence of TTX-R Na+ channels in dural afferent neurons. A. a, Typical traces of TTX-R INa elicited by step pulses (100 msdepolarization pulses from -80 to +30 mV in 10 mV increments at a VH of -80 mV) in small- (left) and medium-sized (right) DiI-positive neurons. b, Conductance-voltage relationships of TTX-R Na+channels in small- (open circles) and medium-sized (closed circles) DiI-positive neurons. The points and error bars represent the mean and SEM from 32 small-sized and 44 medium-sized DiI-positive neurons. The continuous lines represent the best fits using a Boltzmann function. B. Scatter plot of the half-maximal voltage for activation (V50, activation) against membrane capacitance (Cm) (n = 76). The linear trend line represents the best fit using a least-squares fit (r = 0.01). C. Scatter plot of the half-maximal voltage for activation (V50, activation) against the density of TTX-R INaP (n = 76). The linear trend line represents the best fit using a least-squares fit (r = 0.02). D. Typical traces of TTX-R INa elicited by step pulses (100 ms duration; up to -50 mV in 10 mV increments) at VHs of -80 mV (a) or -120 mV (b) in the same small- (left) and medium-sized (right) DiI-positive neurons. Notably, these slowly desensitizing INa, which were mediated by NaV1.9, were elicited when neurons were held at a VH of -120 mV but not of -80 mV. E. Current-voltage relationships of TTX-R Na+ channels in small- (left) and medium-sized (right) DiI-positive neurons. The points and error bars represent the mean and SEM from eight small-sized and seven medium-sized DiI-positive neurons. F. a, Schematic illustration of the voltage step stimulation for steady-state fast inactivation of TTX-R Na+ channels. b, Typical traces of TTX-R INa elicited by step pulses in medium-sized DiI-positive neurons. The inset represents the INaP region (dotted box) with an expanded time scale. c, Current-voltage relationships of TTX-R INaT (open circles) and INaP (closed circles) in medium-sized DiI-positive neurons. Each point represents the mean and SEM from 10experiments. The continuous lines represent the best fits using a Boltzmann function.