Additional file 1 of A novel circRNA, hsa_circ_0069382, regulates gastric cancer progression
Creators: name: Wang, Haoying
name: Yuan, Hao
name: Guo, Qinghong
name: Zeng, Xi
name: Liu, Mengxiao
name: Ji, Rui
name: Chen, Zhaofeng
name: Guan, Quanlin
name: Zheng, Ya
name: Wang, Yuping
name: Zhou, Yongning
Publisher: name: figshare
Year: 2024
Subjects: subject: Biophysics
subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Chemical Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Science Policy

description: Additional file 1: Fig. S1 qRT-PCR examined the outcome of transfecting miR-15a-5p inhibitor in (A) HGC-27, (B) SGC-7901, and (C) AGS cells. qRT-PCR examined the outcome of transfecting miR-15a-5p mimics in (D) HGC-27, (E) SGC-7901, and (F) AGS cells. (G) Graphical representation the miR-15a-5p inhibitor effect on apoptosis of HGC-27 and AGS cells. (H) Graphical representation of the effect of miR-15a-5p inhibitor and mimics on AGS cell cycle. Luciferase reporter gene assays at (I) 24 h and (J) 48 h in miR-15a-5p and BTG2. TCGA data showed low levels of BTG2 in gastric cancer tissues of (K) paired and (L) unpaired samples. (M) Correlation between miR-15a-5p and BTG2 in gastric cancer in TCGA. (N) Correlation between miR- 15a-5p and FAK (PTK2) in gastric cancer in TCGA. (O) Immunohistochemical staining results from the Human Protein Atlas database showed that FAK was highly expressed in gastric cancer. Fig. S2 (A) qRT-PCR for the expression of the homologous linear molecule TBC1D19 of hsa_circ_0069382 in gastric cancer cells. Luciferase reporter gene assays at (B) 24 h and (C) 48 h in miR-15a-5p and hsa_circ_0069382. qRT-PCR results of transfection shows the expression of hsa_circ_0069382 in (D) PEX-3 and hsa_circ_0069382 plasmid transfection group in HGC-27, (E) PEX-3 plasmid, hsa_circ_0069382 plasmid, and miR-15a-5p mimics with hsa_circ_0069382 plasmid transfection group in HGC-27 (F) hsa_circ_0069382 plasmid had no effect on TBC1D19 in HGC-27 (G) The expression of hsa_circ_0069382 in PEX-3 plasmid, hsa_circ_0069382 plasmid, and miR-15a-5p mimics with hsa_circ_0069382 plasmid transfection group in AGS (H) hsa_circ_0069382 plasmid had no effect on TBC1D19 in AGS. The expression of miR-15a-5p in PEX-3 plasmid, hsa_circ_0069382 plasmid, and miR-15a-5p mimics with hsa_circ_0069382plasmid transfection group in (I) HGC-27 and (J) AGS. The expression of BTG2 in PEX-3 plasmid, hsa_circ_0069382 plasmid, and miR-15a-5p mimics with hsa_circ_0069382 plasmid transfection group in (K) HGC-27 and (L) AGS. Fig. S3 Lentivirus transfection and colony formation. The structure of (A) LV3 and (B) LV5. The lentivirus transfection results of (C) AGS and (D) SGC-7901, scale bar, 20μm. (E) Colony formation of AGS. (F), (G) Statistical chart of E. (H) (I) Statistical chart of C, D. Table S1. Target genes of miR-15a-5p. Table S2. The clinical features of 68 gastric cancer patients. Table S3. Prediction of binding sites between miR-15a-5p and circRNA. Table S4. Predicted upstream transcription factors of hsa_circ_0069382. Table S5. Primers used for qRT-PCR, inhibitor and mimic sequences, FISH probe sequences, lentivirus insertion sequences and antibodies. Table S6. Potential IRES for hsa_circ_0069382. Table S7. Potential ORFs for hsa_circ_0069382.