title: ÐÐ°ÑÑÐ¸ÑÑ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ ÑÐ¸Ð±ÑÐ¾Ð¸Ð½Ð° ÑÐµÐ»ÐºÐ° Ð´Ð»Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÐºÐ»ÐµÑÐ¾Ðº
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Silk fibroin matrices for cell culture
Creators: name: ÐÐ¾Ð½ÑÐ³Ð¸Ð½Ð°, ÐÐ¸Ð¾Ð»ÐµÑÑÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: ÑÐ¸Ð±ÑÐ¾Ð¸Ð½Ð¾Ð²ÑÐµ Ð¿Ð»ÐµÐ½ÐºÐ¸
Ð¿Ð¾Ð»Ð¸ÑÑÐ¸Ð»ÐµÐ½Ð³Ð»Ð¸ÐºÐ¾Ð»Ñ
ÑÐºÐ°Ð½ÐµÐ²Ð°Ñ Ð¸Ð½Ð¶ÐµÐ½ÐµÑÐ¸Ñ
Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð°Ð»ÑÐ½ÑÐµ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸
fibroin films
polyethylene glycol
tissue engineering
mesenchymal stem cells

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description: The theme of the final qualifying work: "Matrices based on silk fibroin for cell cultivation." In this work, a technology was developed for obtaining films based on silk fibroin intended for cell cultivation. To create tissue-engineered structures, the method of pouring an aqueous solution of fibroin onto a polystyrene substrate was used. To obtain water-insoluble films, we worked out the conditions for the incubation of films in alcohols. Using IR spectroscopy, the conformational transition from the disordered structure of the untreated film to the Î²-fold structure of the article treated with alcohol was confirmed. During heat treatment of the samples in the temperature range from 30 Â°C to 140 Â°C, a weight loss (&lt;7%) was observed, associated with the release of moisture from the samples. DSC revealed the glass transition temperature of the film untreated with methanol and the film after exposure to methanol, 216 Â°C and 201 Â°C, respectively. The study of mechanical properties showed that, depending on the medium of the experiment: dry or liquid, the film material has different strength and deformation characteristics. Films based on silk fibroin with the addition of PEG or PEG - diamine do not have a toxic effect on the mesenchymal stromal cells of the human bone marrow. All of the above points indicate that fibroin-based film products can be used as tissue-engineered constructs.