title: Ð£ÑÑÑÐ¾Ð¹ÑÑÐ²Ð¾ Ð¿ÑÐ¾Ð±Ð¾Ð¿Ð¾Ð´Ð³Ð¾ÑÐ¾Ð²ÐºÐ¸ Ð´Ð»Ñ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾-Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð¶Ð¸Ð´ÐºÐ¸Ñ ÑÑÐµÐ´
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Sample preparation device for molecular genetic analysis liquid media
Creators: name: ÐÐ¾Ð½Ð¾Ð²Ð°Ð»Ð¾Ð²Ð°, ÐÑÐ±Ð¾Ð²Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Ð¿ÑÐ¾Ð±Ð¾Ð¿Ð¾Ð´Ð³Ð¾ÑÐ¾Ð²ÐºÐ° Ð½ÑÐºÐ»ÐµÐ¸Ð½Ð¾Ð²ÑÑ ÐºÐ¸ÑÐ»Ð¾Ñ
Ð¼Ð¸ÐºÑÐ¾ÑÐ»ÑÐ¸Ð´Ð½Ð¾Ðµ ÑÑÑÑÐ¾Ð¹ÑÑÐ²Ð¾
"Ð¼ÑÐ³ÐºÐ°Ñ" Ð»Ð¸ÑÐ¾Ð³ÑÐ°ÑÐ¸Ñ
ÑÐ¸Ð»Ð¸ÐºÐ¾Ð½Ð¾Ð²Ð°Ñ ÑÐµÐ·Ð¸Ð½Ð°
ÐÐ¦Ð Ð² ÑÐµÐ°Ð»ÑÐ½Ð¾Ð¼ Ð²ÑÐµÐ¼ÐµÐ½Ð¸ (ÐÐ¦Ð -Ð Ð)
sample preparation of nucleic acids
microfluidic device
Â«softÂ» lithography
silicone rubber
realâtime polymerase chain reaction (PCRâRT)

description: ÐÐµÑÐ¾Ð´Ñ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾âÐ³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð¿Ð¾Ð»ÑÑÐ¸Ð»Ð¸ ÑÐ¸ÑÐ¾ÐºÐ¾Ðµ ÑÐ°ÑÐ¿ÑÐ¾ÑÑÑÐ°Ð½ÐµÐ½Ð¸Ðµ Ð² ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ Ð¾Ð±Ð»Ð°ÑÑÑÑ. ÐÐ´Ð½Ð°ÐºÐ¾, Ð´Ð»Ñ Ð½Ð¸Ñ ÑÑÐµÐ±ÑÐµÑÑÑ Ð¿ÑÐ¾Ð±Ð¾Ð¿Ð¾Ð´Ð³Ð¾ÑÐ¾Ð²ÐºÐ°, Ð¾Ð±ÑÑÐ½Ð¾ Ð·Ð°ÐºÐ»ÑÑÐ°ÑÑÐ°ÑÑÑ Ð² Ð»Ð¸Ð·Ð¸ÑÐµ Ð¿ÑÐ¾Ð±Ñ Ð¸ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ð¸ Ð¸Ð· Ð½ÐµÐµ Ð½ÑÐºÐ»ÐµÐ¸Ð½Ð¾Ð²ÑÑ ÐºÐ¸ÑÐ»Ð¾Ñ. ÐÐ¸ÐºÑÐ¾ÑÐ»ÑÐ¸Ð´Ð½ÑÐµ ÑÑÑÑÐ¾Ð¹ÑÑÐ²Ð° Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÑÑ Ð¾Ð±ÑÐµÐ´Ð¸Ð½Ð¸ÑÑ ÑÐ°ÑÑÑ Ð¸Ð»Ð¸ Ð²ÑÐµ ÑÑÐ°Ð´Ð¸Ð¸ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð½Ð° Ð¾Ð´Ð½Ð¾Ð¼ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ð²Ðµ Ð¸ Ð°Ð²ÑÐ¾Ð¼Ð°ÑÐ¸Ð·Ð¸ÑÐ¾Ð²Ð°ÑÑ ÑÑÐ¸ Ð¿ÑÐ¾ÑÐµÑÑÑ, Ð¸ÑÐºÐ»ÑÑÐ¸Ð² Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ ÑÐµÐ»Ð¾Ð²ÐµÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐ°ÐºÑÐ¾ÑÐ° Ð½Ð° ÑÐµÐ·ÑÐ»ÑÑÐ°Ñ. ÐÐ¾ÑÑÐ¾Ð¼Ñ ÑÐµÐ»ÑÑ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÐ»Ð°ÑÑ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð¿ÑÐ¾ÑÐ¾ÑÐ¸Ð¿Ð° ÑÑÑÑÐ¾Ð¹ÑÑÐ²Ð° Ð¿ÑÐ¾Ð±Ð¾Ð¿Ð¾Ð´Ð³Ð¾ÑÐ¾Ð²ÐºÐ¸ Ð¶Ð¸Ð´ÐºÐ¸Ñ ÑÑÐµÐ´, Ð¿ÑÐµÐ´Ð¿Ð¾Ð»Ð°Ð³Ð°ÑÑÐµÐ³Ð¾ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐµÐµ Ð¾Ð±ÑÐµÐ´Ð¸Ð½ÐµÐ½Ð¸Ðµ Ñ ÑÑÑÑÐ¾Ð¹ÑÑÐ²Ð¾Ð¼ Ð´Ð»Ñ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾âÐ³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð°. ÐÑÐ»Ð¾ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð¾ Ð¸ Ð°Ð¿ÑÐ¾Ð±Ð¸ÑÐ¾Ð²Ð°Ð½Ð¾ Ð´Ð²Ð° Ð²Ð°ÑÐ¸Ð°Ð½ÑÐ° ÑÑÑÑÐ¾Ð¹ÑÑÐ²Ð°, Ð¿ÑÐµÐ´Ð½Ð°Ð·Ð½Ð°ÑÐµÐ½Ð½ÑÑ Ð´Ð»Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ñ Ð½Ð°Ð±Ð¾ÑÐ° ÑÐµÐ°Ð³ÐµÐ½ÑÐ¾Ð² Â«ÐâÐ¡Ð¾ÑÐ±âÐÐÐÂ» (ÐÐÐ Â«Ð¡Ð¸Ð½ÑÐ¾Ð»Â»). ÐÑÐ¾ÑÐ¾ÑÐ¸Ð¿Ñ ÑÑÑÑÐ¾Ð¹ÑÑÐ² ÑÐ¾Ð·Ð´Ð°Ð½Ñ Ð¸Ð· ÑÐ¸Ð»Ð¸ÐºÐ¾Ð½Ð¾Ð²Ð¾Ð¹ ÑÐµÐ·Ð¸Ð½Ñ PlatSet 40 Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Â«Ð¼ÑÐ³ÐºÐ¾Ð¹Â» Ð»Ð¸ÑÐ¾Ð³ÑÐ°ÑÐ¸Ð¸ Ñ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐµÐ¹ Ð³ÐµÑÐ¼ÐµÑÐ¸Ð·Ð°ÑÐ¸ÐµÐ¹ ÐºÐ¸ÑÐ»Ð¾ÑÐ¾Ð´Ð½Ð¾Ð¹ Ð¿Ð»Ð°Ð·Ð¼Ð¾Ð¹. ÐÐ¿ÑÐ¾Ð±Ð°ÑÐ¸Ñ ÑÑÑÑÐ¾Ð¹ÑÑÐ² Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð½Ð° Ð¿ÑÐ¸Ð¼ÐµÑÐµ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð½ÑÐºÐ»ÐµÐ¸Ð½Ð¾Ð²ÑÑ ÐºÐ¸ÑÐ»Ð¾Ñ Ð¸Ð· ÐºÐ»ÐµÑÐ¾Ðº E.coli. ÐÑÐ¾Ð´ÐµÐ¼Ð¾Ð½ÑÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð° ÑÐ°Ð±Ð¾ÑÐ¾ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ Ð²ÑÐ¾ÑÐ¾Ð³Ð¾ Ð²Ð°ÑÐ¸Ð°Ð½ÑÐ° ÑÑÑÑÐ¾Ð¹ÑÑÐ²Ð°. ÐÐ´Ð½Ð°ÐºÐ¾, ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ñ Ð²ÑÐ´ÐµÐ»ÑÐµÐ¼ÑÑ ÑÑÑÐµÑÑÐ²ÐµÐ½Ð½ÑÑ ÐºÐ¸ÑÐ»Ð¾Ñ ÑÑÑÑÐ¿Ð°ÐµÑ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½Ð¾Ð¹ Ð¿ÑÐ¸ ÑÑÑÐ½Ð¾Ð¼ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ð¸ (Ð¿Ð¾ÑÐ¾Ð³Ð¾Ð²ÑÐµ ÑÐ¸ÐºÐ»Ñ ÐÐ¦Ð âÐ Ð ÑÐ¾ÑÑÐ°Ð²Ð¸Ð»Ð¸ ÑÐ¸ÑÑÑ Ð¸ ÑÐ¸ÑÑÑ, ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²ÐµÐ½Ð½Ð¾). Ð­ÑÐ¾, Ð²ÐµÑÐ¾ÑÑÐ½Ð¾ ÑÐ²ÑÐ·Ð°Ð½Ð¾ Ñ ÑÐ¾ÑÐ±ÑÐ¸ÐµÐ¹ Ð½Ð° ÑÑÐµÐ½ÐºÐ°Ñ ÐºÐ°Ð½Ð°Ð»Ð¾Ð². ÐÐ»Ñ ÐµÐµ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ñ Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐ°ÑÑ ÑÑÑÑÐ¾Ð¹ÑÑÐ²Ð¾ Ð¿ÐµÑÐµÐ´ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼, Ð½Ð°Ð¿ÑÐ¸Ð¼ÐµÑ, Ð±ÑÑÑÐ¸Ð¼ ÑÑÐ²Ð¾ÑÐ¾ÑÐ¾ÑÐ½ÑÐ¼ Ð°Ð»ÑÐ±ÑÐ¼Ð¸Ð½Ð¾Ð¼.
description: Methods of molecular genetic analysis are widely used in various fields. However, they require sample preparation, usually consisting in lysis of the sample and isolation of nucleic acids from it. Microfluidic devices allow you to combine part or all of the analysis stages on one construct and automate these processes, eliminating the influence of the human factor on the result. Therefore, the aim of the work was to develop a prototype of a sample preparation device for liquid media, involving subsequent integration with a device for molecular genetic analysis. Two versions of the device designed for use with the Â«MâSorbâOOMÂ» reagent kit (Syntol LLC) were developed and tested. Prototypes of the devices were created from silicone rubber PlatSet 40 by the method of "soft" lithography followed by sealing with oxygen plasma. The devices were tested using the example of the isolation of nucleic acids from E.coli cells. The operability of the second version of the device is demonstrated. However, the concentration of the essential acids released is inferior to that obtained by manual isolation (the threshold cycles of PCRâRV were figures and figures, respectively). This is probably due to sorption on the walls of the channels. To reduce it, it is necessary to treat the device before use, for example, with bovine serum albumin.