title: ÐÐµÑÐ¸Ð»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÐÐÐ Ð² Ð³ÐµÐ½Ð°Ñ grin1 Ð¸ hif1Î± Ñ ÐºÑÑÑ Ñ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÐ¼ ÑÑÐ¾Ð²Ð½ÐµÐ¼ Ð²Ð¾Ð·Ð±ÑÐ´Ð¸Ð¼Ð¾ÑÑÐ¸ Ð½ÐµÑÐ²Ð½Ð¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ñ
title: Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÑÐºÐ°Ñ ÑÐ°Ð±Ð¾ÑÐ°
titleType: Subtitle
Creators: name: ÐÑÑÐºÐ¾Ð²Ð°, ÐÐµÑÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2016
Subjects: Ð³ÐµÐ½Ñ
Ð¼ÐµÑÐ¸Ð»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
CpG Ð¾ÑÑÑÐ¾Ð²ÐºÐ¸
ÑÑÑÐµÑÑ
Ð²Ð¾Ð·Ð±ÑÐ´Ð¸Ð¼Ð¾ÑÑÑ
Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿
ÐºÐ¾ÑÑÐ½ÑÐ¹ Ð¼Ð¾Ð·Ð³
ÐºÑÑÑÑ
genes
methylation
CpG islands
stress
excitability
hippocampus
bone marrow
rats

description: ÐÐµÑÐ¾Ð´Ð¾Ð¼ Ð¼ÐµÑÐ¸Ð»ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð½Ð¾Ð¹ ÑÐµÐ¿Ð½Ð¾Ð¹ ÑÐµÐ°ÐºÑÐ¸Ð¸ (ÐÐ¡Ð) Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½ ÑÑÐ°ÑÑÑ Ð¼ÐµÑÐ¸Ð»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¡ÑG Ð¾ÑÑÑÐ¾Ð²ÐºÐ¾Ð² Ð² Ð¿ÑÐ¾Ð¼Ð¾ÑÐ¾ÑÐ°Ñ Ð³ÐµÐ½Ð¾Ð² grin1 Ð¸ hif1Î± Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð³ÐµÐ½Ð¾Ð¼Ð½Ð¾Ð¹ ÐÐÐ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð° Ð¸ ÐºÐ¾ÑÑÐ½Ð¾Ð³Ð¾ Ð¼Ð¾Ð·Ð³Ð° ÐºÑÑÑ Ð»Ð¸Ð½Ð¸Ð¹, ÑÐ°Ð·Ð»Ð¸ÑÐ°ÑÑÐ¸ÑÑÑ Ð¿Ð¾ ÑÑÐ¾Ð²Ð½Ñ Ð²Ð¾Ð·Ð±ÑÐ´Ð¸Ð¼Ð¾ÑÑÐ¸ Ð½ÐµÑÐ²Ð½Ð¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ñ - ÐÐ Ð¸ ÐÐ (ÐÐ - Ð²ÑÑÐ¾ÐºÐ¸Ð¹ Ð¿Ð¾ÑÐ¾Ð³ Ð¸ ÐÐ - Ð½Ð¸Ð·ÐºÐ¸Ð¹ Ð¿Ð¾ÑÐ¾Ð³ Ð²Ð¾Ð·Ð±ÑÐ´Ð¸Ð¼Ð¾ÑÑÐ¸) Ð² Ð½Ð¾ÑÐ¼Ð°Ð»ÑÐ½ÑÑ ÑÑÐ»Ð¾Ð²Ð¸ÑÑ Ð¸ Ð¿Ð¾Ð´ Ð´ÐµÐ¹ÑÑÐ²Ð¸ÐµÐ¼ Ð´Ð»Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ ÑÐ¼Ð¾ÑÐ¸Ð¾Ð½Ð°Ð»ÑÐ½Ð¾-Ð±Ð¾Ð»ÐµÐ²Ð¾Ð³Ð¾ ÑÑÑÐµÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ (ÐÐ­ÐÐ¡). Ð¡ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ NCBI Ð¸ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ñ MethPrimer Ð²ÑÑÐ²Ð»ÐµÐ½Ð¾ 10 Ð¡ÑG Ð¾ÑÑÑÐ¾Ð²ÐºÐ¾Ð² Ð² Ð³ÐµÐ½Ðµ grin1 Ð¸ 8 Ð¡ÑG Ð¾ÑÑÑÐ¾Ð²ÐºÐ¾Ð² Ð² Ð³ÐµÐ½Ðµ hif1Î±. ÐÑÐ±ÑÐ°Ð½Ñ Ð¡ÑG Ð¾ÑÑÑÐ¾Ð²ÐºÐ¸, Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ Ð²Ð°Ð¶Ð½ÑÐµ Ð´Ð»Ñ ÑÐµÐ³ÑÐ»ÑÑÐ¸Ð¸ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð². Ð£ Ð¸Ð½ÑÐ°ÐºÑÐ½ÑÑ ÐºÑÑÑ Ð´Ð²ÑÑ Ð»Ð¸Ð½Ð¸Ð¹ ÐÐ Ð¸ ÐÐ Ð½Ðµ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¾ Ð¼ÐµÐ¶Ð»Ð¸Ð½ÐµÐ¹Ð½ÑÑ ÑÐ°Ð·Ð»Ð¸ÑÐ¸Ð¹ Ð² ÑÑÐ¾Ð²Ð½Ðµ Ð¼ÐµÑÐ¸Ð»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼ÑÑ Ð¡ÑG Ð¾ÑÑÑÐ¾Ð²ÐºÐ¾Ð² Ð³ÐµÐ½Ð¾Ð² grin1 Ð¸ hif1Î±. ÐÑÑÐ²Ð»ÐµÐ½Ñ ÑÐºÐ°Ð½ÐµÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐµÑÐºÐ¸Ðµ ÑÐ°Ð·Ð»Ð¸ÑÐ¸Ñ Ð² ÑÐ²ÑÐ·ÑÐ²Ð°Ð½Ð¸Ð¸ Ñ Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼Ð¾Ð¹ Ð¿Ð°ÑÐ¾Ð¹ Ð¿ÑÐ°Ð¹Ð¼ÐµÑÐ¾Ð² Ðº ÑÑÐ°ÑÑÐºÑ Ð¡ÑG Ð¾ÑÑÑÐ¾Ð²ÐºÐ° Ð¿ÑÐ¾Ð¼Ð¾ÑÐ¾ÑÐ½Ð¾Ð¹ Ð¾Ð±Ð»Ð°ÑÑÐ¸ Ð³ÐµÐ½Ð° grin1.ÐÐ¾Ð´ Ð²Ð»Ð¸ÑÐ½Ð¸ÐµÐ¼ ÐÐ­ÐÐ¡ ÑÐ¾Ð»ÑÐºÐ¾ Ð² ÐºÐ¾ÑÑÐ½Ð¾Ð¼ Ð¼Ð¾Ð·Ð³Ðµ ÐºÑÑÑ Ð»Ð¸Ð½Ð¸Ð¸ ÐÐ Ð¿ÑÐ¾Ð¸ÑÑÐ¾Ð´Ð¸Ñ Ð´ÐµÐ¼ÐµÑÐ¸Ð»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼Ð¾Ð³Ð¾ Ð¡ÑG Ð¾ÑÑÑÐ¾Ð²ÐºÐ° Ð² Ð¿ÑÐ¾Ð¼Ð¾ÑÐ¾ÑÐ½Ð¾Ð¹ Ð¾Ð±Ð»Ð°ÑÑÐ¸ Ð³ÐµÐ½Ð° grin1. Ð Ð³ÐµÐ½Ðµ hif1Ð° ÐÐ­ÐÐ¡ Ð½Ðµ Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÐµÑ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð½Ð° ÑÑÐ°ÑÑÑ Ð¼ÐµÑÐ¸Ð»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼Ð¾Ð³Ð¾ Ð¡ÑG Ð¾ÑÑÑÐ¾Ð²ÐºÐ°.
description: By use of methyl-specific polymeraze chain reaction (MSP)' method we researched the status of methylation of CpG islands in genes grin1 and hif1Î± promoters with a use of genomic DNA of rats' hippocampus and bone marrow with lines varied by the nervous system' excitability - HT and LT (high threshold and low threshold of excitability) in normal conditions and with long-term effect of emotional pain stress (LTEPS). By means of NCBI database and Meth Primer program we educed 10 GpG islands in grin1 gene and 8 CpG islands in gene hiflÎ±. Besides that we chose CpG islands the most important for the regulation genes' expression. Intact rats of two lines (high threshold and low threshold) didn't discover interline difference in methlation level of researched (Ð¾ÑÑÑÐ¾Ð²ÐºÐ¾Ð²) of genes grin1 and hiflÎ±. We also discovered tissue-specific distinction in the way of connection of researched primer-pair to the CpG part of the island of the promotor field of the gene grin1. On the influence of the long-term effect of emotional pain stress demethylation of researched CpG island in promotor field of grin1 gene happens just in rats' bone marrow of the line with a high threshold. Long-term effect of emotional pain stress (LTEPS) in the grin 1 gene doesn't influence on the status of methylation of the researched CpG island.