title: ÐÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾ÑÑÐ¸ ÑÑÐ°Ð¼Ð¼Ð¾Ð² ÑÐ¸Ð·Ð¾Ð±Ð¸Ð¹ ÑÐ¾Ð¸ Ðº ÑÑÑÐµÑÑÐ¾Ð²ÑÐ¼ ÑÐ°ÐºÑÐ¾ÑÐ°Ð¼
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Determination of resistance of soybean rhizobia strains to stress factors
Creators: name: ÐÐ¾Ð»ÑÐºÐ¾Ð², ÐÐ¸ÑÐ¸Ð»Ð»
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: bradyrhizobium japonicum ÑÑ. H9
Ð²Ð°Ð½ÐºÐ¾Ð¼Ð¸ÑÐ¸Ð½
Ð°Ð¼ÑÐ¾ÑÐµÑÐ¸ÑÐ¸Ð½ b
Ð¾ÑÐ¼Ð¾ÑÐ¸ÑÐµÑÐºÐ¸Ð¹ ÑÑÑÐµÑÑ
Ñ Ð¸Ð¼Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÑÑÐµÑÑ
bradyrhizobium japonicum pcs. H9
vancomycin
amphotericin b
osmotic stress
chemical stress

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾ÑÑÐ¸ ÐºÐ»ÑÐ±ÐµÐ½ÑÐºÐ¾Ð²ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ B. japonicum ÑÑ. H9 Ðº Ð¾ÑÐ¼Ð¾ÑÐ¸ÑÐµÑÐºÐ¾Ð¼Ñ, ÑÐ¸Ð¼Ð¸ÑÐµÑÐºÐ¾Ð¼Ñ Ð¸ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð¼Ñ ÑÑÑÐµÑÑÐ°Ð¼ Ð½Ð° Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð½ÑÑ ÑÐµÐ¼ÐµÐ½Ð°Ñ Ð¸ Ð¿ÑÐ¸ Ð½ÐµÑÑÐµÑÐ¸Ð»ÑÐ½Ð¾Ð¼ Ð³Ð»ÑÐ±Ð¸Ð½Ð½Ð¾Ð¼ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ Ð² Ð¿ÑÐ¾Ð¼ÑÑÐ»ÐµÐ½Ð½Ð¾Ð¼ ÑÐµÑÐ¼ÐµÐ½ÑÐµÑ Ñ Ð´Ð¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸ÐµÐ¼ ÑÐµÐ»ÐµÐºÑÐ¸Ð²Ð½ÑÑ Ð°Ð½ÑÐ¸Ð±Ð¸Ð¾ÑÐ¸ÐºÐ¾Ð² - ÐÐ°Ð½ÐºÐ¾Ð¼Ð¸ÑÐ¸Ð½Ð° Ð¸ ÐÐ¼ÑÐ¾ÑÐµÑÐ¸ÑÐ¸Ð½Ð° B.ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: 1. ÐÐ·ÑÑÐ¸ÑÑ ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾ÑÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ B. japonicum ÑÑ. H9 Ðº Ð¾ÑÐ¼Ð¾ÑÐ¸ÑÐµÑÐºÐ¾Ð¼Ñ ÑÑÑÐµÑÑÑ Ð½Ð° Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð½ÑÑ ÑÐµÐ¼ÐµÐ½Ð°Ñ; 2. ÐÐ¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÑÐ³Ð»ÐµÐ²Ð¾Ð´Ð½Ð¾Ð³Ð¾ Ð±Ð¸Ð¾ÑÑÐ°Ð±Ð¸Ð»Ð¸Ð·Ð°ÑÐ¾ÑÐ° ÐºÐ°Ðº ÑÑÐµÐ´ÑÑÐ²Ð° Ð·Ð°ÑÐ¸ÑÑ ÐºÐ»ÐµÑÐ¾Ðº B. japonicum ÑÑ. H9 Ð¾Ñ Ð¾ÑÐ¼Ð¾ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÑÑÐµÑÑÐ°; 3. ÐÐ·ÑÑÐ¸ÑÑ ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾ÑÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ B. japonicum ÑÑ. H9 Ðº ÑÐ¸Ð¼Ð¸ÑÐµÑÐºÐ¾Ð¼Ñ ÑÑÑÐµÑÑÑ Ð½Ð° Ð¿ÑÐ¾ÑÑÐ°Ð²Ð»ÐµÐ½Ð½ÑÑ Ð¿ÐµÑÑÐ¸ÑÐ¸Ð´Ð°Ð¼Ð¸ ÑÐµÐ¼ÐµÐ½Ð°Ñ; 4. ÐÐ·ÑÑÐ¸ÑÑ ÐºÑÐ»ÑÑÑÑÐ°Ð»ÑÐ½ÑÐµ Ð¿ÑÐ¸Ð·Ð½Ð°ÐºÐ¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ B. japonicum ÑÑ. H9, Ð¿Ð¾Ð´Ð¾Ð±ÑÐ°ÑÑ ÐºÐ¾Ð¼Ð¿Ð¾Ð½ÐµÐ½ÑÑ Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ´Ñ Ð¸ Ð¾ÑÑÑÐµÑÑÐ²Ð¸ÑÑ Ð¿ÑÐ¾ÑÐµÑÑ Ð½ÐµÑÑÐµÑÐ¸Ð»ÑÐ½Ð¾Ð³Ð¾ Ð³Ð»ÑÐ±Ð¸Ð½Ð½Ð¾Ð³Ð¾ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÑÐ¸Ð·Ð¾Ð±Ð¸Ð¹ Ð² Ð¿ÑÐ¾Ð¼ÑÑÐ»ÐµÐ½Ð½Ð¾Ð¼ ÑÐµÑÐ¼ÐµÐ½ÑÐµÑÐµ Ñ Ð´Ð¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸ÐµÐ¼ ÑÐµÐ»ÐµÐºÑÐ¸Ð²Ð½ÑÑ Ð°Ð½ÑÐ¸Ð±Ð¸Ð¾ÑÐ¸ÐºÐ¾Ð² - ÐÐ°Ð½ÐºÐ¾Ð¼Ð¸ÑÐ¸Ð½Ð° Ð¸ ÐÐ¼ÑÐ¾ÑÐµÑÐ¸ÑÐ¸Ð½Ð° B. ÐÐ±ÑÐµÐºÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ - ÑÑÐ°Ð¼Ð¼Ñ B. japonicum ÑÑ. H9 Ð¸Ð·Ð²ÐµÐ´Ð¾Ð¼ÑÑÐ²ÐµÐ½Ð½Ð¾Ð¹ ÐºÐ¾Ð»Ð»ÐµÐºÑÐ¸Ð¸ Ð¿Ð¾Ð»ÐµÐ·Ð½ÑÑ Ð¼Ð¸ÐºÑÐ¾Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð¾Ð² ÑÐµÐ»ÑÑÐºÐ¾ÑÐ¾Ð·ÑÐ¹ÑÑÐ²ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð·Ð½Ð°ÑÐµÐ½Ð¸Ñ ÐÑÐµÑÐ¾ÑÑÐ¸Ð¹ÑÐºÐ¾Ð³Ð¾ Ð½Ð°ÑÑÐ½Ð¾-Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÑÐºÐ¾Ð³Ð¾ Ð¸Ð½ÑÑÐ¸ÑÑÑÐ° ÑÐµÐ»ÑÑÐºÐ¾ÑÐ¾Ð·ÑÐ¹ÑÑÐ²ÐµÐ½Ð½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ð¸ (ÐÐÐÐÐ¡Ð¥Ð, Ð³. Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³), Ð¥Ð¸Ð¼Ð¸ÑÐµÑÐºÐ¸Ðµ ÑÑÐ½Ð³Ð¸ÑÐ¸Ð´Ð½ÑÐµ Ð¿ÑÐ¾ÑÑÐ°Ð²Ð¸ÑÐµÐ»Ð¸ Ð¡Ð¸Ð½ÐºÐ»ÐµÑ, ÐÐ¿Ð»Ð¾Ñ, Ð¢Ð¸ÑÐ°Ð´Ð°, ÑÐ³Ð»ÐµÐ²Ð¾Ð´Ð½ÑÐ¹ ÑÑÐ°Ð±Ð¸Ð»Ð¸Ð·Ð°ÑÐ¾Ñ.Â Ð Ð°Ð±Ð¾ÑÐ° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ ÐÑÐµÑÐ¾ÑÑÐ¸Ð¹ÑÐºÐ¾Ð³Ð¾ Ð½Ð°ÑÑÐ½Ð¾ â Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÑÐºÐ¾Ð³Ð¾ Ð¸Ð½ÑÑÐ¸ÑÑÑÐ° ÑÐµÐ»ÑÑÐºÐ¾ÑÐ¾Ð·ÑÐ¹ÑÑÐ²ÐµÐ½Ð½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ð¸. Ð ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ Ð±ÑÐ»Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ñ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ: 1. ÐÐ·ÑÑÐµÐ½Ð° ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾ÑÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ B. japonicum ÑÑ. H9 Ðº Ð¾ÑÐ¼Ð¾ÑÐ¸ÑÐµÑÐºÐ¾Ð¼Ñ ÑÑÑÐµÑÑÑ Ð½Ð° Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð½ÑÑ ÑÐµÐ¼ÐµÐ½Ð°Ñ. 2. ÐÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð° ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÑÐ³Ð»ÐµÐ²Ð¾Ð´Ð½Ð¾Ð³Ð¾ Ð±Ð¸Ð¾ÑÑÐ°Ð±Ð¸Ð»Ð¸Ð·Ð°ÑÐ¾ÑÐ° ÐºÐ°Ðº ÑÑÐµÐ´ÑÑÐ²Ð° Ð·Ð°ÑÐ¸ÑÑ ÐºÐ»ÐµÑÐ¾Ðº B. japonicum ÑÑ. H9 Ð¾Ñ Ð¾ÑÐ¼Ð¾ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÑÑÐµÑÑÐ°. 3. ÐÐ·ÑÑÐµÐ½Ð° ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾ÑÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ B. japonicum ÑÑ. H9 Ðº ÑÐ¸Ð¼Ð¸ÑÐµÑÐºÐ¾Ð¼Ñ ÑÑÑÐµÑÑÑ Ð½Ð° Ð¿ÑÐ¾ÑÑÐ°Ð²Ð»ÐµÐ½Ð½ÑÑ Ð¿ÐµÑÑÐ¸ÑÐ¸Ð´Ð°Ð¼Ð¸ ÑÐµÐ¼ÐµÐ½Ð°Ñ. 4. ÐÐ·ÑÑÐµÐ½Ñ ÐºÑÐ»ÑÑÑÑÐ°Ð»ÑÐ½ÑÐµ Ð¿ÑÐ¸Ð·Ð½Ð°ÐºÐ¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ B. japonicum ÑÑ. H9, Ð¿Ð¾Ð´Ð¾Ð±ÑÐ°Ð½Ñ ÐºÐ¾Ð¼Ð¿Ð¾Ð½ÐµÐ½ÑÑ Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ´Ñ Ð¸ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÐµÐ½Ñ Ð¿ÑÐ¾ÑÐµÑÑÑ Ð½ÐµÑÑÐµÑÐ¸Ð»ÑÐ½Ð¾Ð³Ð¾ Ð³Ð»ÑÐ±Ð¸Ð½Ð½Ð¾Ð³Ð¾ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÑÐ¸Ð·Ð¾Ð±Ð¸Ð¹ Ð² Ð¿ÑÐ¾Ð¼ÑÑÐ»ÐµÐ½Ð½Ð¾Ð¼ ÑÐµÑÐ¼ÐµÐ½ÑÐµÑÐµ Ñ Ð´Ð¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸ÐµÐ¼ ÑÐµÐ»ÐµÐºÑÐ¸Ð²Ð½ÑÑ Ð°Ð½ÑÐ¸Ð±Ð¸Ð¾ÑÐ¸ÐºÐ¾Ð² - ÐÐ°Ð½ÐºÐ¾Ð¼Ð¸ÑÐ¸Ð½Ð° Ð¸ ÐÐ¼ÑÐ¾ÑÐµÑÐ¸ÑÐ¸Ð½Ð° B.
description: This work is devoted to the study of the resistance of nodule bacteria B. japonicum pcs. H9 to osmotic, chemical and biological stresses on treated seeds and during non-sterile deep culturing in an industrial fermenter with the addition of selective antibacterial agents - Vancomycin and Amphotericin B. Tasks that were solved during the study: 1. To study the resistance of B. japonicum H9 bacteria to osmotic stress on treated seeds; 2. To determine the effectiveness of a carbohydrate biostabilizer as a means of protecting B. japonicum H9 cells from osmotic stress; 3. To study the resistance of B. japonicum P. H9 bacteria to chemical stress on pesticide-etched seeds; 4. To study the cultural characteristics of bacteria B. japonicum pcs. H9, to obtain the components of the nutrient medium and to carry out the process of unsterile deep cultivation of rhizobia in an industrial fermenter with the addition of selective antibiotics - Vancomycin and Amphotericin B. The object of research is strains of B. japonicum pcs. H9 from the departmental collection of useful microorganisms of agricultural significance of the All-Russian Research Institute of Agricultural Microbiology (VNIISHM, St. Petersburg), Chemical fungicidal protectants Sinclair, Oplot, Tirade, carbohydrate stabilizer. The work was carried out based on the laboratory of the AllâRussian Research Institute of Agricultural Microbiology. In the course of the research, the following results were obtained: 1. The resistance of B. japonicum H9 bacteria to osmotic stress on treated seeds were studied. 2. The effectiveness of a carbohydrate biostabilizer as a means of protecting B. japonicum H9 cells from osmotic stress was determined. 3. The resistance of B. japonicum H9 bacteria to chemical stress on pesticide-etched seeds were studied. 4. The cultural characteristics of the bacteria B. japonicum pcs. H9 have been studied, the components of the nutrient medium have been obtained and the processes of unstable deep cultivation of rhizobia in an industrial enzyme with the addition of selective antibiotics - Vancomycin and Amphotericin B.