Additional file 1 of Antifungal bio-coating of endotracheal tube built by overexpressing the MCP1 gene of Saccharomyces boulardii and employing hydrogel as a “house” to antagonize Candida albicans
Creators: name: Wei, Yunyun
name: Qiu, Jianfeng
name: Han, Ziqiang
name: Wang, Xuanyi
name: Zhang, Hui
name: Hou, Xinya
name: Lv, Xiangwei
name: Mao, Xiaolong
Publisher: name: figshare
Year: 2023
Subjects: subject: Space Science
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Infectious Diseases
subject: FOS: Health sciences

description: Additional file 1: Figure S1. (A) S. boulardii inhibit adhesion of C. albicans. S. boulardii and C. albicans were mixed and cultured in polyethylene culture dish for 1 h. Bar = 10 μm. (B) Crystal violet staining method was used to determine the adhesion of C. albicans. (C) S. boulardii inhibition the hyphal development of C. albicans. Bar = 10 μm. (D) Determination the growth of hyphal. The length of the hypha is measured by Image J. Statistical significance was defined based on the different p-values: *p &lt; 0.05, **p &lt; 0.01, and ***p &lt; 0.001. Figure S2. Compression and bending resistance tests of GelMA hydrogel. Figure S3. (A) The images of GelMA + PEO hydrogel and rhodamine B-labelled GelMA + PEO hydrogel. (B) Pore size distribution of GelMA+PEO hydrogel. Figure S4. Viability determination of B16 cells. The Calcein-AM, PI staining and merge images of B16 cells cultured respectively with DMSO and the extraction solution of GelMA and GelMA + PEO hydrogel. (Green remarked live cells, red remarked dead cells). Bar = 50 μm. Figure S5. (A) A full scan of Fig. 3G (Mcp1-GFP) entire original gel; (B) A full scan of Fig. 3G (Tubulin) entire original gel. Figure S6. Leaching test of S. boulardii encapsulated in coating (A) The images of bio-coatings containing S. boulardii and transgenic S. bourlardii were immersed in physiological saline (0.9% NaCl) for 4 days, respectively. (B) Photos of S. boulardii and transgenic S. bourlardii colonies on agar broth plates separated from the soaking solution with different bio-coating. Figure S7. In vivo implanted studies (A) Photos of S. boulardii hydrogels in vitro and in vivo, and the attachment tissue sections with different hydrogels derived from mice. (B) The cell survival rate of S. boulardii and transgenic S. boulardii encapsulated in hydrogels after implanted in vivo for 4 days. Statistical significance was defined based on the different p-values: *p &lt; 0.05, **p &lt; 0.01, and ***p &lt; 0.001. Figure S8. (A) Calcein-AM/PI staining of S. boulardii in bioactive materials under condition of UV at 180 min. (Green remarked live cells, red remarked dead cells) Bar = 50 μm. (B) The cells survival rate of S. boulardii in bioactive materials under condition of UV at 30, 60, 120, and 180 min. Statistical significance was defined based on the different p-values: *p &lt; 0.05, **p &lt; 0.01, and ***p &lt; 0.001. Figure S9. Overexpression of MCP1 enhances mitochondrial function of S. boulardii in bioactive materials after Co-incubation with C. albicans. (A) The expression levels of mitochondrial related genes ATP15 and SHH4 of S. boulardi revealed by RT-PCR. Expression levels of electron transport chain related genes CYC1, CYC7, CYT1 and SDH3 revealed by RT-PCR. ACT1 was used as the normalization gene. Statistical significance was defined based on the different p-values: *p &lt; 0.05, **p &lt; 0.01, and ***p &lt; 0.001.