title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ Ð²Ð°ÑÐ¸Ð°ÑÐ¸Ð¹ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÐ¸ Ð¨Ð°Ð¹Ð½Ð°-ÐÐ°Ð»ÑÐ³Ð°ÑÐ½Ð¾ Ð¼Ð ÐÐ Ð½Ð° ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð±Ð¸Ð¾ÑÐ¸Ð½ÑÐµÐ·Ð° Ð±ÐµÐ»ÐºÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Influence of variations in the Shine-Dalgarno mRNA sequence on the efficiency of protein biosynthesis
Creators: name: Ð¨Ð°Ð¼Ð°ÑÐ´Ð¸Ð½Ð°, ÐÐ°Ð´Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Ð±Ð¸Ð¾ÑÐ¸Ð½ÑÐµÐ· Ð±ÐµÐ»ÐºÐ°
Ð¸Ð½Ð¸ÑÐ¸Ð°ÑÐ¸Ñ ÑÑÐ°Ð½ÑÐ»ÑÑÐ¸Ð¸
Ð¼Ð ÐÐ
Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÑ Ð¨Ð°Ð¹Ð½Ð°-ÐÐ°Ð»ÑÐ³Ð°ÑÐ½Ð¾
5â-Ð½ÐµÑÑÐ°Ð½ÑÐ»Ð¸ÑÑÐµÐ¼Ð°Ñ Ð¾Ð±Ð»Ð°ÑÑÑ
70S Ð¸Ð½Ð¸ÑÐ¸Ð°ÑÐ¾ÑÐ½ÑÐ¹ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑ
Ð¾ÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð½ÑÐ¹ Ð¿Ð¾ÑÐ¾Ðº
protein biosynthesis
translation initiation
mRNA
Shine-Dalgarno sequence
5â-untranslated region
70S initiation complex
stopped flow

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«ÐÐ»Ð¸ÑÐ½Ð¸Ðµ Ð²Ð°ÑÐ¸Ð°ÑÐ¸Ð¹ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÐ¸ Ð¨Ð°Ð¹Ð½Ð°-ÐÐ°Ð»ÑÐ³Ð°ÑÐ½Ð¾ Ð¼Ð ÐÐ Ð½Ð° ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð±Ð¸Ð¾ÑÐ¸Ð½ÑÐµÐ·Ð° Ð±ÐµÐ»ÐºÐ°Â». Ð¦ÐµÐ»Ñ ÑÐ°Ð±Ð¾ÑÑ: Ð°Ð½Ð°Ð»Ð¸Ð· Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹ Ð² Ð´Ð»Ð¸Ð½Ðµ Ð¸Ð½Ð¸ÑÐ¸Ð°ÑÐ¾ÑÐ½Ð¾Ð¹ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÐ¸ Ð¨Ð°Ð¹Ð½Ð°-ÐÐ°Ð»ÑÐ³Ð°ÑÐ½Ð¾ (SD) Ð¼Ð ÐÐ, Ð° ÑÐ°ÐºÐ¶Ðµ Ð² Ð½ÑÐºÐ»ÐµÐ¾ÑÐ¸Ð´Ð½Ð¾Ð¼ ÑÐ°ÑÑÑÐ¾ÑÐ½Ð¸Ð¸ Ð¼ÐµÐ¶Ð´Ñ SD Ð¸ AUG ÑÑÐ°ÑÑÐ¾Ð²ÑÐ¼ ÐºÐ¾Ð´Ð¾Ð½Ð¾Ð¼ Ð½Ð° Ð¿Ð°ÑÑÐ¸Ð°Ð»ÑÐ½ÑÐµ ÑÐµÐ°ÐºÑÐ¸Ð¸ Ð¿ÑÐ¾ÑÐµÑÑÐ° ÑÑÐ°Ð½ÑÐ»ÑÑÐ¸Ð¸ invitro. Ð Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÐµÐ½Ð½ÑÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ñ Ð±ÑÐ»Ð¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÑ Ð¼Ð ÐÐ, ÐºÐ¾Ð´Ð¸ÑÑÑÑÐ¸Ðµ Ð»Ð°Ð·ÑÑÐ½ÑÐ¹ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½ÑÐ¹ Ð±ÐµÐ»Ð¾Ðº Cerulean (CER) Ñ Ð²Ð½ÐµÑÐµÐ½Ð½ÑÐ¼Ð¸ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸ÑÐ¼Ð¸ Ð² 5â-Ð½ÐµÑÑÐ°Ð½ÑÐ»Ð¸ÑÑÐµÐ¼Ð¾Ð¹ Ð¾Ð±Ð»Ð°ÑÑÐ¸ (5â-UTR) Ð³ÐµÐ½Ð°. ÐÑÐ¿Ð¾Ð»ÑÐ·ÑÑ Ð¼ÐµÑÐ¾Ð´Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð°, Ð³ÐµÐ»Ñ-ÑÐ¸Ð»ÑÑÑÐ°ÑÐ¸Ð¸, Ð¾Ð±ÑÐ°ÑÐ½Ð¾ÑÐ°Ð·Ð½Ð¾Ð¹ ÑÑÐ¾Ð¼Ð°ÑÐ¾Ð³ÑÐ°ÑÐ¸Ð¸ Ð¸ Ð¾ÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¿Ð¾ÑÐ¾ÐºÐ°, Ð² ÑÐ°Ð¼ÐºÐ°Ñ Ð´Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¾ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÐ¸ SD Ð½Ð° ÑÑÐ°Ð´Ð¸Ñ Ð¸Ð½Ð¸ÑÐ¸Ð°ÑÐ¸Ð¸, ÑÐ²ÑÐ·ÑÐ²Ð°Ð½Ð¸Ðµ Ð°Ð¼Ð¸Ð½Ð¾Ð°ÑÐ¸Ð»-ÑÐ ÐÐ Ð² Ð ÑÐ°Ð¹Ñ ÑÐ¸Ð±Ð¾ÑÐ¾Ð¼Ñ Ð¸ Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¿ÐµÑÐ²Ð¾Ð¹ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð½Ð¾Ð¹ ÑÐ²ÑÐ·Ð¸. ÐÑÐºÑÑÑÐ¸Ðµ Ð½Ð¾Ð²ÑÑ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð¾Ð² ÑÐµÐ³ÑÐ»ÑÑÐ¸Ð¸ ÑÑÐ°Ð½ÑÐ»ÑÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð¸Ð¼ÐµÐµÑ Ð²ÑÑÐ¾ÐºÑÑ Ð°ÐºÑÑÐ°Ð»ÑÐ½Ð¾ÑÑÑ Ð² Ð³ÐµÐ½Ð½Ð¾Ð¹ Ð¸Ð½Ð¶ÐµÐ½ÐµÑÐ¸Ð¸, Ð° ÑÐ°ÐºÐ¶Ðµ Ð² ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐµ Ð½Ð¾Ð²ÑÑ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² ÑÐµÑÐ°Ð¿Ð¸Ð¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÑ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¹. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð²Ð½Ð¾ÑÑÑ Ð²ÐºÐ»Ð°Ð´ Ð² Ð¿Ð¾Ð½Ð¸Ð¼Ð°Ð½Ð¸Ðµ ÑÑÐ½Ð´Ð°Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð¾ÑÐ½Ð¾Ð² Ð¿ÑÐ¾ÑÐµÑÑÐ° ÑÑÐ°Ð½ÑÐ»ÑÑÐ¸Ð¸, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¾ÑÐºÑÑÐ²Ð°ÑÑ Ð½Ð¾Ð²ÑÐµ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾ÑÑÐ¸ Ð² Ð±Ð¾ÑÑÐ±Ðµ Ñ Ð±Ð¾Ð»ÐµÐ·Ð½ÐµÑÐ²Ð¾ÑÐ½ÑÐ¼Ð¸ Ð¼Ð¸ÐºÑÐ¾Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð°Ð¼Ð¸.
description: Topic of the final qualification work: "Influence of variations in the Shine-Dalgarno mRNA sequence on the efficiency of protein biosynthesis". The aim of this work was to analyze the effect of changes in the length of the Shine-Dalgarno (SD) mRNA initiation sequence, as well as in the nucleotide distance between the SD and AUG start codons, on the partial reactions of the translation process in vitro. In the experiments presented, mRNA transcripts encoding the azure fluorescent protein Cerulean (CER) with changes in the 5â-untranslated region (5â-UTR) of the gene were used. Using fluorescence analysis, gel filtration, reverse phase chromatography, and stopped flow methods, this study determined the effect of SD sequence changes on the initiation step, binding of aminoacyl-tRNA to the A site of the ribosome, and formation of the first peptide bond. The discovery of new mechanisms for the regulation of translational efficiency is of high relevance in genetic engineering, as well as in the development of new methods of therapy. The results obtained contribute to the understanding of the fundamental principles of the translation process, as well as open up new possibilities in the fight against pathogens.