Plasma response to a single dose of dietary βcryptoxanthin esters from papaya (Carica papaya L.) or non-esterified β-cryptoxanthin in adult human subjects: A comparative study
Creators: name: Breithaupt, Dietmar E.
name: Weller, Philipp
name: Wolters, Maike
name: Hahn, Andreas
Publisher: name: Cambridge : Cambridge University Press
Year: 2003
Subjects: subject: β-Cryptoxanthin
subject: Carotenoid ester
subject: Liquid chromatography-atmospheric pressure chemical ionization-mass spectroscopy
subject: Plasma carotenoid response
subject: beta cryptoxanthin
subject: cholesterol
subject: cryptoxanthin
subject: ester
subject: triacylglycerol
subject: unclassified drug
subject: yoghurt
subject: antineoplastic agent
subject: beta carotene
subject: drug derivative
subject: xanthophyll
subject: adult
subject: area under the curve
subject: article
subject: bioavailability
subject: blood level
subject: blood sampling
subject: cholesterol blood level
subject: clinical article
subject: clinical trial
subject: comparative study
subject: controlled clinical trial
subject: controlled study
subject: crossover procedure
subject: diet restriction
subject: dietary intake
subject: esterification
subject: female
subject: high performance liquid chromatography
subject: human
subject: human experiment
subject: male
subject: mass spectrometry
subject: meal
subject: normal human
subject: papaya
subject: randomized controlled trial
subject: single blind procedure
subject: standardization
subject: triacylglycerol blood level
subject: blood
subject: chemistry
subject: diet supplementation
subject: intestine absorption
subject: methodology
subject: Carica
subject: Carica papaya
subject: Adult
subject: Anticarcinogenic Agents
subject: beta Carotene
subject: Cholesterol
subject: Chromatography, High Pressure Liquid
subject: Cross-Over Studies
subject: Dietary Supplements
subject: Esters
subject: Female
subject: Humans
subject: Intestinal Absorption
subject: Male
subject: Single-Blind Method
subject: Triglycerides
subject: Xanthophylls
subject: Yogurt
subject: Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit

description: Many orange-coloured fruits contain β-cryptoxanthin in its non-esterified as well as its esterified form. Information concerning the absorption of β-cryptoxanthin, especially with regard to the metabolism of its fatty acid esters, is rather scarce. The present study assessed the plasma concentration reached after consumption of a single dose of native β-cryptoxanthin esters from papaya (Carica papaya L.) or non-esterified β-cryptoxanthin in equal total amounts. In a randomized, single-blind crossover study, twelve subjects were served a portion of yoghurt containing esterified or non-esterified β-cryptoxanthin (1.3 mg absolute) together with a balanced breakfast. Between the two intervention days, there was a 2-week depletion period. After a fasting blood sample had been taken, futher samples were taken from the subjects at 3, 6, 9, 12 and 24 h. The concentration of non-esterified β-cryptoxanthin in the whole plasma was determined by HPLC; β-cryptoxanthin identification was confirmed by liquid chromatography-atmospheric pressure chemical ionization-MS analyses. Irrespective of the consumed diet, the plasma β-cryptoxanthin concentrations increased significantly (P=0.05) and peaked after 6-12h. The concentration curves, as well as the areas under the curves, were not distinguishable according to two-sided F and t tests (P=0.05). Standardization of β-cryptoxanthin concentrations to plasma triacylglycerol and cholesterol had no impact on the results. Thus, the present study indicates comparable bioavailability of both non-esterified β-cryptoxanthin and mixtures of β-cryptoxanthin esters. The results support the existence of an effective enzymatic cleavage system accepting various p-cryptoxanthin esters.