cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Regulation of Cell Signaling Dynamics by the Protein Kinase-Scaffold Ste5
Creators: name: Behar, Marcelo
name: Elston, Timothy C.
name: Errede, Beverly
name: Dohlman, Henrik G.
name: Shanks, Ryan H.
name: Hao, Nan
name: Hasty, Jeffrey
name: Nayak, Sujata
name: Nagiec, Michal J.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2008

description: Cell differentiation requires the ability to detect and respond appropriately to a variety of extracellular signals. Here we investigate a differentiation switch induced by changes in the concentration of a single stimulus. Yeast cells exposed to high doses of mating pheromone undergo cell division arrest. Cells at intermediate doses become elongated and divide in the direction of a pheromone gradient (chemotropic-growth). Either of the pheromone-responsive MAP kinases, Fus3 and Kss1, promotes cell elongation, but only Fus3 promotes chemotropic growth. Whereas Kss1 is activated rapidly and with a graded dose-response profile, Fus3 is activated slowly and exhibits a steeper dose-response relationship (ultrasensitivity). Fus3 activity requires the scaffold protein Ste5; when binding to Ste5 is abrogated Fus3 behaves like Kss1, and the cells no longer respond to a gradient or mate efficiently with distant partners. We propose that scaffold proteins serve to modulate the temporal and dose-response behavior of the MAP kinase.
A Core Complex of CPSF73, CPSF100, and Symplekin May Form Two Different Cleavage Factors for Processing of Poly(A) and Histone mRNAs
Creators: name: Marzluff, William F.
name: Steiniger, Mindy
name: Sullivan, Kelly D.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2009

description: Metazoan histone mRNAs are unique: their pre-mRNAs contain no introns and the mRNAs are not polyadenylated ending instead in a conserved stem-loop structure. In Drosophila, canonical poly(A) signals are located downstream of the normal cleavage site of each histone gene, and are utilized when histone 3’end formation is inhibited. Here we define a sub-complex of poly(A) factors required for histone pre-mRNA processing. We demonstrate that Symplekin, CPSF73 and CPSF100 are present in a stable complex and interact with histone specific processing factors. We use chromatin immunoprecipitation to show that Symplekin and CPSF73, but not CstF50, cotranscriptionally associate with histone genes. Depletion of SLBP recruits CstF50 to histone genes. Knockdown of CPSF160 or CstF64 downregulates Symplekin but does not affect histone pre-mRNA processing or association of Symplekin with the histone locus. These results suggest that a common core cleavage factor is required for processing of histone and polyadenylated pre-mRNAs.
Charmonium Transverse Momentum Distribution in High Energy Nuclear Collisions
Creators: name: Tang, Zebo
name: Xu, Nu
name: Zhou, Kai
name: Zhuang, Pengfei
Publisher: name: arXiv
Year: 2014
Subjects: subject: Nuclear Theory (nucl-th)
subject: FOS: Physical sciences

description: The Charmonium transverse momentum distribution is more sensitive to the nature of the hot QCD matter created in high energy nuclear collisions, in comparison with the yield. Taking a detailed transport approach for charmonium motion together with a hydrodynamic description for the medium evolution, the cancelation between the two hot nuclear matter effects, the dissociation and the regeneration, controls the charmonium transverse momentum distribution. Especially, the second moment of the distribution can be used to differentiate between the hot mediums produced at SPS, RHIC and LHC energies.
description: 22 pages, 7 figures; Review. Accepted by JPG
Patterns of language use in Malawi
Creators: name: Kayambazinthu, Edrinnie Lora
Publisher: name: La Trobe
Year: 2023

description: This paper reports on results of a sociolinguistic investigation on patterns of language use in Malawi. The analysis which was limited to seven domains of interaction revealed two main patterns of language use. One pattern involving intraethnic or ingroup communication encoded ethnic identity, familiarity and traditional status quo. Another pattern involving interethnic or outgroup communication encoded social distance and interpersonal interactions along power and hierarchical differences. The results in general showed that language use in Malawi-is fluid and not compartmentalised. Based on these findings the paper attempts to argue for the importance of integrating macro and micro-level sociolinguistic theories to account for the large corpus of data that showed use of more than one language in a particular domain or speech event.
Testament von Johannes Amerbach
Creators: name: Amerbach, Johannes
Publisher: name: [s.n.]
Year: 1513

description: Kauf 1661 Öffentliche Bibliothek der Universität Basel
description: Lateinisch
description: Druck: Die Amerbachkorrespondenz / bearb. und hrsg. von Alfred Hartmann, Beat Rudolf Jenny ... [et al.]. - Basel : Verlag der Universitätsbibliothek, 1942-2010. - Bd. 1, Nr. 491a, S. 467ff.
description: Verzeichnung=Description=Inventaire Mai 2015 Hans Berner
Drosophila MUS312 and the Vertebrate Ortholog BTBD12 Interact with DNA Structure-Specific Endonucleases in DNA Repair and Recombination
Creators: name: LaRocque, Jeannine R.
name: Moore, Chris B.
name: Bergstralh, Daniel T.
name: Andersen, Sabrina L.
name: Sekelsky, Jeff
name: Kohl, Kathryn P.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2009

description: DNA recombination and repair pathways require structure-specific endonucleases to process DNA structures that include forks, flaps, and Holliday junctions. Previously, we determined that the Drosophila MEI-9-ERCC1 endonuclease interacts with the novel MUS312 protein to produce meiotic crossovers, and that MUS312 has a MEI-9-independent role in interstrand crosslink (ICL) repair. The importance of MUS312 to pathways crucial for maintaining genomic stability in Drosophila prompted us to search for orthologs in other organisms. Based on sequence, expression pattern, conserved protein-protein interactions, and ICL repair function, we determined that the mammalian ortholog of MUS312 is BTBD12. Orthology between these proteins and S. cerevisiae Slx4 helped identify a conserved interaction with a second structure-specific endonuclease, SLX1. Genetic and biochemical evidence described here and in related papers suggest that MUS312 and BTBD12 direct Holliday junction resolution by at least two distinct endonucleases in different recombination and repair contexts.
Beam pattern evolution of accreting X-ray pulsar 1A 0535+262 during its 2020 giant outburst
Creators: name: Hu, Y. F.
name: Ji, L.
name: Yu, C.
name: Wang, P. J.
name: Doroshenko, V.
name: Santangelo, A.
name: Saathoff, I.
name: Zhang, S. N.
name: Zhang, S.
name: Kong, L. D.
Publisher: name: arXiv
Year: 2023
Subjects: subject: High Energy Astrophysical Phenomena (astro-ph.HE)
subject: FOS: Physical sciences

description: We report on pulse profile decomposition analysis of a bright transient X-ray pulsar 1A 0535+262 using the broadband Insight-HXMT observations during a giant outburst of the source in 2020. We show that the observed pulse profile shape can be described in terms of a combination of two symmetric single-pole contributions for wide range of energies and luminosities for a fixed geometry defining basic geometry of the pulsar. This corresponds to a slightly distorted dipole magnetic field, i.e., one pole has to be offset by $\sim 12^{\circ}$ from the antipodal position of the other pole. We reconstruct the intrinsic beam patterns of the pulsar assuming the geometry recovered from the decomposition analysis, and find evidence for a transition between "pencil" and "fan" beams in energy ranges above the cyclotron line energy which can be interpreted as transition from sub- to super-critical accretion regimes associated with onset of an accretion column. At lower energies the beam pattern appears, however, to be more complex, and contains substantial "fan" beam and an additional "pencil" beam component at all luminosities. The latter is not related to the accretion rate and is stronger in the fading phase of the outburst. We finally discuss results in context of other observational and theoretical findings earlier reported for the source in the literature.
description: 14 pages, 8 figures. Accepted by ApJ
Strings aplicadas às bases - MSL
Creators: name: Da Costa Junior, Alfredo Lima
name: Da Silva, Karen Sulliany Souza
name: Araújo, Fabíola Pantoja Oliveira
Publisher: name: Zenodo
Year: 2022

description: Documento que possibilita a visualização da planilha que descreve as strings aplicadas a cada uma das bases de busca do trabalho Uso da Aprendizagem Tangencial Através de Jogos: Um Mapeamento Sistemático da Literatura.
Role of CD73 in patients with advanced melanoma treated with anti-PD-1 agents
Creators: name: Turiello, Roberta
Publisher: name: Universita degli studi di Salerno
Year: 2022
Subjects: subject: BIO/14 FARMACOLOGIA
subject: Melanoma
subject: Immunoterapia

description: 2020 - 2021
description: The treatment of metastatic melanoma was revolutionized by the approval of inhibitors of immune-checkpoints, including antibodies targeting the programmed cell death protein 1 (PD- 1), such as nivolumab and pembrolizumab. Anti PD-1 agents improved the survival of patients with advanced melanoma; however, a great percentage of patients do not benefit from treatment with these drugs and understanding the mechanisms influencing the response to anti PD-1 agents is an urgent need. Extracellular adenosine is a potent anti-inflammatory mediator able to impair anti-tumor immune response. The adenosine pathway has been indicated as one of the mechanisms causing immune suppression and resistance to immune-checkpoint inhibitors. The main enzyme responsible for extracellular adenosine production is the ectonucleotidase CD73, which hydrolyzes AMP into adenosine and inorganic phosphate. CD73 is anchored to the membrane of many cell types, and its expression is upregulated in several human cancers. This ectonucleotidase is also found on the membrane of exosomes, extracellular vesicles (30-150 nm) that are involved in cell-to-cell communication and are produced by almost all cell types, including cancer cells and immune cells. CD73 can be cleaved from the cell membrane and the soluble form is free to circulate in biological fluids. The main goal of this PhD project was to investigate the potential of all the forms of CD73 as predictive factors of response in patients with advanced melanoma receiving anti- PD-1 agents (nivolumab, pembrolizumab) alone or in combination with anti CTLA-4 (ipilimumab). The project was divided in four parts, each one focused on a different form of CD73: the cell-bound form expressed by circulating lymphocytes, the soluble and the exosomal form in serum and the cell-bound form within the melanoma lesion. .. [edited by Author]
FLASH, a Proapoptotic Protein Involved in Activation of Caspase-8, Is Essential for 3′ End Processing of Histone Pre-mRNAs
Creators: name: Yan, Yan
name: Yang, Xiao-cui
name: Burch, Brandon D.
name: Dominski, Zbigniew
name: Marzluff, William F.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2009

description: 3′ end processing of histone pre-mRNA requires U7 snRNP, which binds downstream of the cleavage site and recruits the endonuclease CPSF-73. U7 snRNP contains a unique Sm ring in which the canonical SmD2 protein is replaced by Lsm11. We used the yeast two-hybrid system to identify binding partners of Lsm11 and selected the pro-apoptotic protein FLASH. Human FLASH interacts with Lsm11 in vitro and stimulates 3′ end processing of histone pre-mRNA in mammalian nuclear extracts. We also identified the FLASH ortholog in Drosophila and demonstrate that it interacts with Lsm11 in vitro and in vivo. Drosophila FLASH localizes to histone locus bodies and its depletion in fly cells inhibits U7-dependent processing resulting in polyadenylation of histone mRNAs. These results demonstrate that FLASH is an essential factor required for 3′ end maturation of histone mRNAs in both vertebrates and invertebrates and suggest a potential link between this process and apoptosis.
Matching branches of non-perturbative conformal block at its singularity divisor
Creators: name: Itoyama, H.
name: Mironov, A.
name: Morozov, A.
Publisher: name: arXiv
Year: 2014
Subjects: subject: High Energy Physics - Theory (hep-th)
subject: FOS: Physical sciences

description: Conformal block is a function of many variables, usually represented as a formal series, with coefficients which are certain matrix elements in the chiral (e.g. Virasoro) algebra. Non-perturbative conformal block is a multi-valued function, defined globally over the space of dimensions, with many branches and, perhaps, additional free parameters, not seen at the perturbative level. We discuss additional complications of non-perturbative description, caused by the fact that all the best studied examples of conformal blocks lie at the singularity locus in the moduli space (at divisors of the coefficients or, simply, at zeroes of the Kac determinant). A typical example is the Ashkin-Teller point, where at least two naive non-perturbative expressions are provided by elliptic Dotsenko-Fateev integral and by the celebrated Zamolodchikov formula in terms of theta-constants, and they are different. The situation is somewhat similar at the Ising and other minimal model points.
description: 28 pages
Highly Transcribed RNA Polymerase II Genes Are Impediments to Replication Fork Progression in Saccharomyces cerevisiae
Creators: name: Giresi, Paul G.
name: Zakian, Virginia A.
name: Azvolinsky, Anna
name: Lieb, Jason D.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2009

description: Replication forks face multiple obstacles that slow their progression. By two-dimensional gel analysis, yeast forks pause at stable DNA protein complexes, and this pausing is greatly increased in the absence of the Rrm3 helicase. We used a genome wide approach to identify 96 sites of very high DNA polymerase binding in wild type cells. Most of these binding sites were not previously identified pause sites. Rather, the most highly represented genomic category among high DNA polymerase binding sites was the open reading frames (ORFs) of highly transcribed RNA polymerase II genes. Twice as many pause sites were identified in rrm3 compared to wild type cells as pausing in this strain occurred at both highly transcribed RNA polymerase II genes and the previously identified protein DNA complexes. ORFs of highly transcribed RNA polymerase II genes are the first class of natural pause sites that are not exacerbated in rrm3 cells.
The discovery of blue-cored dwarf early-type galaxies in isolated environments
Creators: name: Rey, Soo-Chang
name: Kim, Suk
name: Chung, Jiwon
name: Lee, Youngdae
Publisher: name: arXiv
Year: 2023
Subjects: subject: Astrophysics of Galaxies (astro-ph.GA)
subject: FOS: Physical sciences

description: The presence of blue-cored dwarf early-type galaxies (dE(bc)s) in high-density environments supports the scenario of the transformation of infalling late-type galaxies into quiescent dwarf early-type galaxies by environmental effects. While low-density environments lacking environmental processes could not be relevant to the formation of dE(bc)s, we discovered a large sample of rare dE(bc)s in isolated environments at z < 0.01 using the NASA-Sloan Atlas catalog. Thirty-two isolated dE(bc)s were identified by visual inspection of the Sloan Digital Sky Survey images and g - r color profiles. We found that (1) isolated dE(bc)s exhibit similar structural parameters to dE(bc)s in the Virgo cluster; (2) based on the ultraviolet-r color-magnitude relation, color gradients, and optical emission lines of dE(bc)s, isolated dE(bc)s show more vigorous, centrally concentrated SF compared to their counterparts in the Virgo cluster; (3) at a given stellar mass, isolated dE(bc)s tend to have a larger fraction of gas mass than their Virgo counterparts. We discuss a scenario of episodic SF sustained by gas accretion, suggested by Sanchez Almeida et al., in which the star-bursting blue compact dwarf galaxy (BCD)-quiescent BCD (QBCD) cycle can be repeated during the Hubble time. We suggest that, in this cadence, isolated dE(bc)s might be QBCDs at pre- or post-BCD stages. Our results imply that dE(bc)s comprise a mixture of objects with two types of origins, nature or nurture, depending on their environment.
description: 8 pages, 5 figures, accepted for publication in Astrophysical Journal
Plants and processes in wetlands: a background
Creators: name: Boon, Paul I
name: Brock, Margaret A
Publisher: name: La Trobe
Year: 2023

description: Murray-Darling Freshwater Research Centre

MDFRC item.

It is easy to gain an impression from the recent contents of Australian scientific journals dealing with ecological research that little attention is paid to the botanical ecology of Australia's inland wetlands. Less than 1% of the papers published in key Australian ecological journals over 1987-93 dealt with some aspect of the vegetation ecology of these environments. Yet over the period 1982-94 research on this topic accounted for up to 23% of the papers presented at annual conferences of the two major Australian scientific societies to which Australian limnologists are likely to belong. This discrepancy indicates that wetland vegetation is the subject of a considerable research effort by Australian limnologists, but that few of their research findings are published in refereed Australian journals. Analyses of the references cited in key review articles suggests that refereed journals outside Australia cannot account fully for the 'missing' publications: we demonstrate that much research is placed in the largely inaccessible 'grey literature' published by government departments and the like. It is also possible that some research is destined never to be published. This imbalance between the intensity of the research effort and the history of publication in Australian refereed journals prompted local scientists involved in wetland research to participate in a Special Issue dedicated to the botanical and process-oriented aspects of wetland ecology.
Systematic Analysis of Essential Genes Reveals Important Regulators of G Protein Signaling
Creators: name: Dohlman, Henrik G.
name: Baker, Rachael
name: Skowyra, Dorota
name: Cappell, Steven D.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2010

description: The yeast pheromone pathway consists of a canonical heterotrimeric G protein and MAP kinase cascade. To identify new signaling components we systematically evaluated 870 essential genes using a library of repressible-promoter strains. Quantitative transcription-reporter and MAPK activity assays were used to identify strains that exhibit altered pheromone sensitivity. Of the 92 newly identified essential genes required for proper G protein signaling, those involved with protein degradation were most highly-represented. Included in this group are members of the SCF (Skp-Cullin-F-Box) ubiquitin ligase complex. Further genetic and biochemical analysis reveals that SCFCdc4 acts together with the Cdc34 ubiquitin conjugating enzyme at the level of the G protein, promotes degradation of the G protein α subunit, Gpa1, in vivo and catalyzes Gpa1 ubiquitination in vitro. These new insights to the G protein signaling network reveal the essential-genome as an untapped resource for identifying new components and regulators of signal transduction pathways.
Six-dimensional weak-strong simulations of head-on beam-beam compensation in RHIC
Creators: name: Luo, Y.
name: Fischer, W.
name: Abreu, N. P.
name: Gu, X.
name: Pikin, A.
name: Robert-Demolaize, G.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Accelerator Physics (physics.acc-ph)
subject: FOS: Physical sciences

description: To compensate the large beam-beam tune spread and beam-beam resonance driving terms in the polarized proton operation in the Relativistic Heavy Ion Collider (RHIC), we will introduce a low-energy DC electron beam into each ring to collide head-on with the opposing proton beam. The device to provide the electron beam is called an electron lens. In this article, using a 6-D weak-strong-beam-beam interaction simulation model, we investigate the effects of head-on beam-beam compensation with electron lenses on the proton beam dynamics in the RHIC 250 GeV polarized proton operation. This article is abridged from the published article [1].
description: 5 pages, contribution to the ICFA Mini-Workshop on Beam-Beam Effects in Hadron Colliders, CERN, Geneva, Switzerland, 18-22 Mar 2013
Acetylation Regulates Gluconeogenesis by Promoting PEPCK1 Degradation via Recruiting the UBR5 Ubiquitin Ligase
Creators: name: Zhao, Shimin
name: Lei, Qunying
name: Lin, Yan
name: Zhou, Lisha
name: Xiao, Mengtao
name: Wang, Shiwen
name: Jiang, Wenqing
name: Guan, Kun-Liang
name: Xiong, Yue
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: Protein acetylation has emerged as a major mechanism in regulating cellular metabolism. Whereas most glycolytic steps are reversible, the reaction catalyzed by pyruvate kinase is irreversible and the reverse reaction requires phosphoenolpyruvate carboxykinase (PEPCK1) to commit for gluconeogenesis. Here we show that acetylation regulates the stability of the gluconeogenic rate limiting enzyme PEPCK1, thereby modulating cellular response to glucose. High glucose destabilizes PEPCK1 by stimulating its acetylation. PEPCK1 is acetylated by the P300 acetyltransferase and this acetylation stimulates the interaction between PEPCK1 and UBR5, a HECT domain containing E3 ubiquitin ligase, therefore promoting PEPCK1 ubiquitinylation and degradation. Conversely, SIRT2 deacetylates and stabilizes PEPCK1. These observations represent an example that acetylation targets a metabolic enzyme to a specific E3 ligase in response to metabolic condition changes. Given that increased levels of PEPCK is linked with type II diabetes, this study also identifies potential therapeutic targets for diabetes.
Acetylation Targets the M2 Isoform of Pyruvate Kinase for Degradation through Chaperone-Mediated Autophagy and Promotes Tumor Growth
Creators: name: Lei, Qun-Ying
name: Huang, Yiran
name: Li, Zi
name: Zha, Zhengyu
name: Lv, Lei
name: Li, Dong
name: Lin, Ruiting
name: Guan, Kun-Liang
name: Xiong, Yue
name: Xu, Yanping
name: Zhao, Di
name: Wang, Gang
name: Liu, Ying
name: Zhang, Heng
name: Chu, Yajing
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: Most tumor cells take up more glucose than normal cells but metabolize glucose via glycolysis even in the presence of normal levels of oxygen, a phenomenon known as the Warburg effect. Tumor cells commonly express the embryonic M2 isoform of pyruvate kinase (PKM2) that may contribute to the metabolism shift from oxidative phosphorylation to aerobic glycolysis and tumorigenesis. Here we show that PKM2 is acetylated on lysine 305 and that this acetylation is stimulated by high glucose concentration. PKM2 K305 acetylation decreases PKM2 enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy (CMA). Acetylation increases PKM2 interaction with HSC70, a chaperone for CMA, and association with lysosomes. Ectopic expression of an acetylation mimetic K305Q mutant accumulates glycolytic intermediates and promotes cell proliferation and tumor growth. These results reveal an acetylation regulation of pyruvate kinase and the link between lysine acetylation and CMA.
Preschoolers' classification and induction of word meaning
Creators: name: Kelly, Barbara
Publisher: name: La Trobe
Year: 2023

description: This paper reports the results of two experiments examining preschoolers’ induction of word meaning and their ability to extend word meanings at the basic and superordinate levels The first experiment examined preschoolers’ classification of unfamiliar animate and inanimate objects according, to superordinate categories and inductions regarding their ability for self-generated movement. Three and four-year-olds showed some ability to classify both animate and inanimate objects at the superordinate level and to make inductions about movement from this classification. The second experiment examined preschoolers’ extension of word meaning across objects with the same basic category label. Children aged three and four indicated some ability to make inductions about an unfamiliar object when its category label was known, but the results for the two age groups was not significant.
Direct, Noncatalytic Mechanism of IKK Inhibition by A20
Creators: name: Chen, Zhijian J.
name: Skaug, Brian
name: Chen, Jueqi
name: Ma, Averil
name: He, Jin
name: Du, Fenghe
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: A20 is a potent anti-inflammatory protein that inhibits NF-κB, and A20 dysfunction is associated with autoimmunity and B-cell lymphoma. A20 harbors a deubiquitination enzyme domain and can employ multiple mechanisms to antagonize ubiquitination upstream of NEMO, a regulatory subunit of the IκB kinase complex (IKK). However, direct evidence of IKK inhibition by A20 is lacking, and the inhibitory mechanism remains poorly understood. Here we show that A20 can directly impair IKK activation without deubiquitination or impairment of ubiquitination enzymes. We find that polyubiquitin binding by A20, which is largely dependent on A20’s seventh zinc finger motif (ZnF7), induces specific binding to NEMO. Remarkably, this ubiquitin-induced recruitment of A20 to NEMO is sufficient to block IKK phosphorylation by its upstream kinase TAK1. Our results suggest a non-catalytic mechanism of IKK inhibition by A20 and a means by which polyubiquitin chains can specify a signaling outcome.
Accounting for non-ideal mixing effects in the hydrogen-helium equation of state
Creators: name: Howard, Saburo
name: Guillot, Tristan
Publisher: name: arXiv
Year: 2023
Subjects: subject: Earth and Planetary Astrophysics (astro-ph.EP)
subject: FOS: Physical sciences

description: The equation of state for hydrogen and helium is fundamental for studying stars and giant planets. It has been shown that because of interactions at atomic and molecular levels, the behaviour of a mixture of hydrogen and helium cannot be accurately represented by considering these elements separately. This paper aims at providing a simple method to account for interactions between hydrogen and helium in interior and evolution models of giant planets. Using on the one hand ab initio simulations that involve a system of interacting hydrogen and helium particles and pure equations of state for hydrogen and helium on the other, we derived the contributions in density and entropy of the interactions between hydrogen and helium particles. We show that relative variations of up to 15% in density and entropy arise when non-ideal mixing is accounted for. These non-ideal mixing effects must be considered in interior models of giant planets based on accurate gravity field measurements, particularly in the context of variations in the helium-to-hydrogen ratio. They also affect the mass-radius relation of exoplanets. We provide a table that contains the volume and entropy of mixing as a function of pressure and temperature. This table is to be combined with pure hydrogen and pure helium equations of state to obtain an equation of state that self-consistently includes mixing effects for any hydrogen and helium mixing ratio and may be used to model the interior structure and evolution of giant planets to brown dwarfs. Non-linear mixing must be included in accurate calculations of the equations of state of hydrogen and helium. Uncertainties on the equation of state still exist, however. Ab initio calculations of the behaviour of the hydrogen-helium mixture in the megabar regime for various compositions should be performed in order to gain accuracy.
Essential Role for Ubiquitin-Ubiquitin-Conjugating Enzyme Interaction in Ubiquitin Discharge from Cdc34 to Substrate
Creators: name: Kuhlman, Brian
name: Lewis, Steven
name: Deshaies, Raymond J.
name: Saha, Anjanabha
name: Kleiger, Gary
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: During ubiquitin conjugation, the thioester bond that links ‘donor’ ubiquitin to ubiquitin-conjugating enzyme (E2) undergoes nucleophilic attack by the ε-amino group of an acceptor lysine, resulting in formation of an isopeptide bond. Models of ubiquitination have envisioned the donor ubiquitin to be a passive participant in this process. However, we show here that the I44A mutation in ubiquitin profoundly inhibits its ability to serve as a donor for ubiquitin chain initiation or elongation, but can be rescued by computationally-predicted compensatory mutations in the E2 Cdc34. The donor defect of ubiquitin-I44A can be partially suppressed either by using a low pKa amine (hydroxylamine) as the acceptor or by performing reactions at higher pH, suggesting that the discharge defect arises in part due to inefficient deprotonation of the acceptor lysine. We propose that interaction between Cdc34 and the donor ubiquitin organizes the active site to promote efficient ubiquitination of substrate.
Subgame-Perfect Equilibria in Stochastic Timing Games
Creators: name: Riedel, Frank
name: Steg, Jan-Henrik
Publisher: name: arXiv
Year: 2014
Subjects: subject: Optimization and Control (math.OC)
subject: Probability (math.PR)
subject: FOS: Mathematics
subject: 60G40, 91A25, 91A40, 91A55

description: We introduce a notion of subgames for stochastic timing games and the related notion of subgame-perfect equilibrium in possibly mixed strategies. While a good notion of subgame-perfect equilibrium for continuous-time games is not available in general, we argue that our model is the appropriate version for timing games. We show that the notion coincides with the usual one for discrete-time games. Many timing games in continuous time have only equilibria in mixed strategies -- in particular preemption games, which often occur in the strategic real option literature. We provide a sound foundation for some workhorse equilibria of that literature, which has been lacking as we show. We obtain a general constructive existence result for subgame-perfect equilibria in preemption games and illustrate our findings by several explicit applications.
description: 27 pages, 1 figure
Maps of secular resonances in the NEO region
Creators: name: Fenucci, M.
name: Gronchi, G. F.
name: Novaković, B.
Publisher: name: arXiv
Year: 2023
Subjects: subject: Earth and Planetary Astrophysics (astro-ph.EP)
subject: Mathematical Physics (math-ph)
subject: FOS: Physical sciences

description: Context. From numerical simulations, it is known that some secular resonances may affect the motion of near-Earth objects (NEOs). However, the specific location of the secular resonance inside the NEO region is not fully known, because the methods previously used to predict their location can not be used for highly eccentric orbits and when the NEOs cross the orbits of the planets. Aims. In this paper, we aim to map the secular resonances with the planets from Venus to Saturn in the NEO region, even for high values of the eccentricity. Methods. We used an averaged semi-analytical model that can deal with orbit crossing singularities for the computation of the secular dynamics of NEOs, from which we can obtain suitable proper elements and proper frequencies. Then, we computed the proper frequencies over a uniform grid in the proper elements space. Secular resonances are thus located by the level curves corresponding to the proper frequencies of the planets. Results. We determined the location of the secular resonances with the planets from Venus to Saturn, showing that they appear well inside the NEO region. By using full numerical N-body simulations we also showed that the location predicted by our method is fairly accurate. Finally, we provided some indications about possible dynamical paths inside the NEO region, due to the presence of secular resonances.
description: Accepted for publication in A&A
NF-κB Induction of the SUMO Protease SENP2: A Negative Feedback Loop to Attenuate Cell Survival Response to Genotoxic Stress
Creators: name: Lee, Moon Hee
name: Yeh, Edward T.H.
name: Miyamoto, Shigeki
name: Mabb, Angela M.
name: Gill, Grace B.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: Activation of NF-κB, pivotal for immunity and oncogenesis, is tightly controlled by multiple feedback mechanisms. In response to DNA damage, SUMOylation of NEMO (NF-κB essential modulator) is critical for NF-κB activation, however SUMO proteases and feedback mechanisms involved remain unknown. Here we show that among the six known SENPs (Sentrin/SUMO-specific proteases) only SENP2 can efficiently associate with NEMO, deSUMOylate NEMO and inhibit NF-κB activation induced by DNA damage. We further show that NF-κB induces SENP2 (and SENP1) transcription selectively in response to genotoxic stimuli, which involves ATM (ataxia telangiectasia mutated)-dependent histone methylation of SENP2 promoter κB regions and NF-κB recruitment. SENP2-null cells display biphasic NEMO SUMOylation and activation of IKK and NF-κB, and higher resistance to DNA damage-induced cell death. Our study establishes a self-attenuating feedback mechanism selective to DNA damage induced signaling to limit NF-κB-dependent cell survival responses.
Rotating Ayón-Beato-García black hole as a particle accelerator
Creators: name: Ghosh, Sushant G.
name: Sheoran, Pankaj
name: Amir, Muhammed
Publisher: name: arXiv
Year: 2014
Subjects: subject: General Relativity and Quantum Cosmology (gr-qc)
subject: High Energy Astrophysical Phenomena (astro-ph.HE)
subject: High Energy Physics - Theory (hep-th)
subject: FOS: Physical sciences

description: We study the collision of two particles with equal masses moving in the equatorial plane near horizon of the rotating regular Ayón-Beato-García (ABG) black hole (BH) and calculate the center-of-mass (CM) energy for the colliding particles for both extremal and non-extremal cases. It turns out that CM energy depends not only on rotation parameter $a$ but also on charge $Q$. Particularly for the extremal rotating regular ABG BH, CM energy of two colliding particles could be arbitrarily high for critical angular momentum of particles. Furthermore, we also show that, for a non-extremal BH, there exist a finite upper limit of CM energy, which changes with charge $Q$. A comparison, with Kerr and Kerr-Newman black holes, is included.
description: 11 pages, 13 figures, 2 tables, accepted for publication in Physical Review D
Differential Interferometric Signatures of Close Binaries of Supermassive Black Holes in Active Galactic Nuclei: II. Merged Broad Line Regions
Creators: name: Songsheng, Yu-Yang
name: Wang, Jian-Min
Publisher: name: arXiv
Year: 2023
Subjects: subject: Astrophysics of Galaxies (astro-ph.GA)
subject: FOS: Physical sciences

description: Pairs of supermassive black holes (SMBHs) at different stages are natural results of galaxy mergers in the hierarchical framework of galaxy formation and evolution. However, identifications of close binaries of SMBHs (CB-SMBHs) with sub-parsec separations in observations are still elusive. Recently, unprecedented spatial resolutions achieved by GRAVITY/GRAVITY+ onboard The Very Large Telescope Interferometer through spectroastrometry (SA) provide new opportunities to resolve CB-SMBHs. Differential phase curves of CB-SMBHs with two independent broad-line regions (BLRs) are found to have distinguished characteristic structures from a single BLR \citep{songsheng2019}. Once the CB-SMBH evolves to the stage where BLRs merge to form a circumbinary BLR, it will hopefully be resolved by the pulsar timing array (PTA) in the near future as sources of nano-hertz gravitational waves. In this work, we use a parameterized model for circumbinary BLRs to calculate line profiles and differential phase curves for SA observations. We show that both profiles and phase curves exhibit asymmetries caused by the Doppler boosting effect of accretion disks around individual black holes, depending on the orbital parameters of the binary and geometries of the BLR. We also generate mock SA data using the model and then recover orbital parameters by fitting the mock data. Degeneracies between parameters contribute greatly to uncertainties of parameters but can be eased through joint analysis of multiple-epoch SA observations and reverberation mappings.
description: 21 pages, 17 figures, accepted by ApJ
Rate of larval development of Pantala flavescens (Fabricius) at its southern limit of range in Australia (Anisoptera: Libellulidae)
Creators: name: Hawking, J. H
name: Ingram, B. A
Publisher: name: La Trobe
Year: 2023

description: Murray-Darling Freshwater Research Centre

MDFRC item.

The larvae of Pantala flavescens completed egg and larval development in less than 51 days in fish farm ponds, at a latitude 2 degree south of its previously known distributional range in southern Australia. The development rate was less than recorded in earlier studies. Larvae of a second generation were collected from the ponds until May, and were absent until the following February. This evidence indicates that the larvae can complete a life cycle, but their progeny do not overwinter, and that the new population each year is probably derived from migratory adults.
Untangling Mixed Architectural Plans. Chapter 1. Cerro Santa Lucia. Ghosts in the shells
Creators: name: delgado ruz, isabel
Publisher: name: figshare
Year: 2022
Subjects: subject: Behavioral Neuroscience
subject: Neuroscience and Physiological Psychology
subject: Neuroscience
subject: 110999 Neurosciences not elsewhere classified
subject: FOS: Clinical medicine

description: In this book Untangling Architectural Plans we present different aspects of World history that have been represented on Chilean architecture and urban planning.
Probing the Active Massive Black Hole Candidate in the Center of NGC 404 with VLBI
Creators: name: Paragi, Z.
name: Frey, S.
name: Kaaret, P.
name: Cseh, D.
name: Overzier, R.
name: Kharb, P.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Astrophysics of Galaxies (astro-ph.GA)
subject: High Energy Astrophysical Phenomena (astro-ph.HE)
subject: FOS: Physical sciences

description: Recently Nyland et al. (2012) argued that the radio emission observed in the center of the dwarf galaxy NGC 404 originates in a low-luminosity active galactic nucleus (LLAGN) powered by a massive black hole ($M\sim<10^6$ M$_{\odot}$). High-resolution radio detections of MBHs are rare. Here we present sensitive, contemporaneous Chandra X-ray, and very long baseline interferometry (VLBI) radio observations with the European VLBI Network (EVN). The source is detected in the X-rays, and shows no long-term variability. If the hard X-ray source is powered by accretion, the apparent low accretion efficiency would be consistent with a black hole in the hard state. Hard state black holes are known to show radio emission compact on the milliarcsecond scales. However, the central region of NGC 404 is resolved out on 10 milliarcsecond (0.15-1.5 pc) scales. Our VLBI non-detection of a compact, partially self-absorbed radio core in NGC 404 implies that either the black hole mass is smaller than $3^{+5}_{-2}\times10^5$ M$_{\odot}$, or the source does not follow the fundamental plane of black hole activity relation. An alternative explanation is that the central black hole is not in the hard state. The radio emission observed on arcsecond (tens of pc) scales may originate in nuclear star formation or extended emission due to AGN activity, although the latter would not be typical considering the structural properties of low-ionization nuclear emission-line region galaxies (LINERs) with confirmed nuclear activity.
description: Accepted for publication in the Astrophysical Journal. 7 pages, 2 figures, 1 table
The effect of thermal non-equilibrium on kinetic nucleation
Creators: name: Kiefer, Sven
name: Gobrecht, David
name: Decin, Leen
name: Helling, Christiane
Publisher: name: arXiv
Year: 2023
Subjects: subject: Earth and Planetary Astrophysics (astro-ph.EP)
subject: Astrophysics of Galaxies (astro-ph.GA)
subject: Solar and Stellar Astrophysics (astro-ph.SR)
subject: FOS: Physical sciences

description: Nucleation is considered to be the first step in dust and cloud formation in the atmospheres of asymptotic giant branch (AGB) stars, exoplanets, and brown dwarfs. In these environments dust and cloud particles grow to macroscopic sizes when gas phase species condense onto cloud condensation nuclei (CCNs). Understanding the formation processes of CCNs and dust in AGB stars is important because the species that formed in their outflows enrich the interstellar medium. Although widely used, the validity of chemical and thermal equilibrium conditions is debatable in some of these highly dynamical astrophysical environments. We aim to derive a kinetic nucleation model that includes the effects of thermal non-equilibrium by adopting different temperatures for nucleating species, and to quantify the impact of thermal non-equilibrium on kinetic nucleation. Forward and backward rate coefficients are derived as part of a collisional kinetic nucleation theory ansatz. The endothermic backward rates are derived from the law of mass action in thermal non-equilibrium. We consider elastic collisions as thermal equilibrium drivers. For homogeneous TiO2 nucleation and a gas temperature of 1250 K, we find that differences in the kinetic cluster temperatures as small as 20 K increase the formation of larger TiO2 clusters by over an order of magnitude. An increase in cluster temperature of around 20 K at gas temperatures of 1000 K can reduce the formation of a larger TiO2 cluster by over an order of magnitude. Our results confirm and quantify the prediction of previous thermal non-equilibrium studies. Small thermal non-equilibria can cause a significant change in the synthesis of larger clusters. Therefore, it is important to use kinetic nucleation models that include thermal non-equilibrium to describe the formation of clusters in environments where even small thermal non-equilibria can be present.
description: 13 pages, 4 figures
PTB-Anforderungen 5.02 „Lagerbehälter“. Ausgabe September 2022
Creators: name: Physikalisch-Technische Bundesanstalt (PTB)
Publisher: name: Physikalisch-Technische Bundesanstalt (PTB)
Year: 2022
Subjects: PTB-Anforderungen
Volumen
Messgeräte zur Bestimmung des Volumens
Lagerbehälter
PTB-A 5.02

description: PTB-Anforderungen enthalten Anforderungen zu technischen Spezifikationen und Verwendungspflichten für Messgeräte, die den Regelungen des Mess- und Eichrechts unterliegen. Sie werden von der PTB unter Beteiligung der betroffenen Kreise erstellt. PTB-Anforderungen bestehen aus zwei Teilen. Der erste Teil behandelt Regeln und technische Spezifikationen für Messgeräte, um die wesentlichen Anforderungen an Messgeräte nach § 6 des Mess- und Eichgesetzes i. V. m. § 7 der Mess- und Eichverordnung zu konkretisieren. Der zweite Teil behandelt Regeln und Erkenntnisse zur näheren Bestimmung der Pflichten von Personen, die Messgeräte oder Messwerte verwenden, nach §§ 31 und 33 Mess- und Eichgesetz und §§ 22 und 23 Mess- und Eichverordnung. Diese PTB-Anforderungen enthalten Konkretisierungen für die Teile 1 und 2 für folgende Messgeräte nach § 1 Absatz 1 Nummer 5 der Mess- und Eichverordnung zur Bestimmung des Volumens: Lagerbehälter. Diese PTB-Anforderungen befassen sich nicht mit separaten automatischen Füllstandsmessgeräten oder Peilbandmaßen. Diese PTB-Anforderungen ersetzen die bisherigen PTB-A 5.02 der Ausgabe Juli 2020.
X-Linked Mental Retardation Gene CUL4B Targets Ubiquitylation of H3K4 Methyltransferase Component WDR5 and Regulates Neuronal Gene Expression
Creators: name: Xiong, Yue
name: Nakagawa, Tadashi
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: CUL4B, encoding a scaffold protein for the assembly of Cullin4B-Ring ubiquitin ligase (CRL4B) complexes, is frequently mutated in X-linked mental retardation (XLMR) patients. Here, we show that CUL4B, but not its paralogue CUL4A, targets WDR5, a core subunit of histone H3 lysine 4 (H3K4) methyltransferase complexes, for ubiquitination and degradation in the nucleus. Knocking down CUL4B increases WDR5 and trimethylated H3K4 (H3K4me3) on the neuronal gene promoters and induces their expression. Furthermore, CUL4B depletion suppresses neurite outgrowth of PC12 neuroendocrine cells which can be rescued by co-depletion of WDR5. XLMR-linked mutations destabilize CUL4B and impair its ability to support neurite outgrowth of PC12 cells. Our results identify WDR5 as a critical substrate of CUL4B in regulating neuronal gene expression and suggest epigenetic change as a common pathogenic mechanism for CUL4B-associated XLMR.
The first Delta a observations of three globular clusters
Creators: name: Paunzen, E.
name: Iliev, I. Kh.
name: Pintado, O. I.
name: Baum, H.
name: Maitzen, H. M.
name: Netopil, M.
name: Oenehag, A.
name: Zejda, M.
name: Fraga, L.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Solar and Stellar Astrophysics (astro-ph.SR)
subject: Instrumentation and Methods for Astrophysics (astro-ph.IM)
subject: FOS: Physical sciences

description: Globular clusters are main astrophysical laboratories to test and modify evolutionary models. Thought to be rather homogeneous in their local elemental Distribution of members, results suggest a wide variety of chemical peculiarities. Besides different main sequences, believed to be caused by different helium abundances, peculiarities of blue horizontal-branch stars and on the red giant branch were found. This whole zoo of peculiar objects has to be explained in the context of stellar formation and evolution. The tool of Delta a photometry is employed in order to detect peculiar stars in the whole spectral range. This three filter narrow band system measures the flux distribution in the region from 4900 to 5600A in order to find any peculiarities around 5200A. It is highly efficient to detect classical chemically peculiar stars of the upper main sequence, Be/Ae, shell and metal-weak objects in the Milky Way and Magellanic Clouds. We present Delta a photometry of 2266 stars from 109 individual frames for three globular Clusters (NGC 104, NGC 6205, and NGC 7099). A comparison with published abundances, for three horizontal-branch stars, only, yield an excellent agreement. According to the 3 sigma detection limit of each globular cluster, about 3% of the stars lie in abnormal regions in the diagnostic diagrams. The first observations of three widely different aggregates give very promising results, which will serve as a solid basis for follow-up observations including photometric as well as spectroscopic studies.
description: 8 pages, 5 figures, MNRAS, 443, 2492 (2014)
Algebraic observers & fixed-time estimation using Modulating Functions
Creators: name: Noack, Matti
Publisher: name: Zenodo
Year: 2022
Subjects: Modulating Functions
Algebraic observers
Linearization
Auxiliary problem

description: The seminar deals with different concepts of Modulating Function based algebraic observers. After an introduction to the underlying operator form, different estimation tasks are outlined. The considered algebraic observers include the classical approach using UMFs, the usage of observability canonical form as well as the construction via auxiliary problems. Based on that, a linearization framework for a class of nonlinear systems is established. Furthermore, the convolvible nonlinearity perspective is introduced and sketched for robotic problems.