title: ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð¸Ð¾Ð½Ð¾Ð² ÐºÐ°Ð»ÑÑÐ¸Ñ Ð½Ð° ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° ÑÐ½Ð´Ð¾Ð³ÐµÐ½Ð½ÑÑ ÐºÐ°Ð»ÑÑÐ¸Ð¹-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼ÑÑ Ñ Ð»Ð¾ÑÐ½ÑÑ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² ANO6 Ð² ÐºÐ»ÐµÑÐºÐ°Ñ H293T
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Study of the effect of calcium ions on the electrophysiological properties of endogenous calcium-activated chloride channels ANO6 in HEK293T cells
Creators: name: Ð ÐµÑÐµÑÐ¸Ð½, ÐÐ¼Ð¸ÑÑÐ¸Ð¹
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: ÐºÐ°Ð»ÑÑÐ¸Ð¹-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼ÑÐµ Ñ Ð»Ð¾ÑÐ½ÑÐµ ÐºÐ°Ð½Ð°Ð»Ñ
Ð¼ÐµÑÐ¾Ð´ Ð»Ð¾ÐºÐ°Ð»ÑÐ½Ð¾Ð¹ ÑÐ¸ÐºÑÐ°ÑÐ¸Ð¸ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð°
ÑÐµÐ³Ð¸ÑÑÑÐ°ÑÐ¸Ñ ÑÐ¾ÐºÐ¾Ð² ÑÐµÑÐµÐ· Ð¾Ð´Ð¸Ð½Ð¾ÑÐ½ÑÐµ ÐºÐ°Ð½Ð°Ð»Ñ
calcium-activated chloride channels
patch-clamp
single channels recording

description: ÐÐµÐ»ÐºÐ¸ ÑÐµÐ¼ÐµÐ¹ÑÑÐ²Ð° Ð°Ð½Ð¾ÐºÑÐ°Ð¼Ð¸Ð½Ð¾Ð² (ANO) ÑÐ¾ÑÐ¼Ð¸ÑÑÑÑ ÐºÐ°Ð»ÑÑÐ¸Ð¹-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼ÑÐµ ÑÐ»Ð¾ÑÐ½ÑÐµ ÐºÐ°Ð½Ð°Ð»Ñ (CaCC). ÐÐ°Ð¸Ð±Ð¾Ð»ÐµÐµ Ð¸Ð½ÑÐµÑÐµÑÐ½ÑÐ¼ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð¸ÑÐµÐ»ÐµÐ¼ ÑÐµÐ¼ÐµÐ¹ÑÑÐ²Ð° ÑÐ²Ð»ÑÐµÑÑÑ ANO6 (TMEM16F). ANO6 Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÑÐµÑ ÑÐ¾Ð±Ð¾Ð¹ ÑÑÐ°Ð½ÑÐ¼ÐµÐ¼Ð±ÑÐ°Ð½Ð½ÑÐ¹ Ð±ÐµÐ»Ð¾Ðº, Ð¾Ð±Ð»Ð°Ð´Ð°ÑÑÐ¸Ð¹ ÑÑÐ½ÐºÑÐ¸ÑÐ¼Ð¸ Ð»Ð¸Ð¿Ð¸Ð´Ð½Ð¾Ð¹ ÑÐºÑÐ°Ð¼Ð±Ð»Ð°Ð·Ñ Ð¸ Ð¸Ð¾Ð½Ð½Ð¾Ð³Ð¾ ÐºÐ°Ð½Ð°Ð»Ð°. ANO6 ÑÑÐ°ÑÑÐ²ÑÐµÑ Ð² Ð¼Ð¾Ð´ÑÐ»ÑÑÐ¸Ð¸ Ð¿Ð»Ð¾ÑÐ°Ð´Ð¸ Ð¿Ð»Ð°Ð·Ð¼Ð°ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ñ Ð¸ ÑÐµÐ³ÑÐ»ÑÑÐ¸Ð¸ ÑÑÐ°Ð½ÑÐ¼ÐµÐ¼Ð±ÑÐ°Ð½Ð½Ð¾Ð³Ð¾ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð°. ÐÐ°ÑÑÑÐµÐ½Ð¸Ñ ÑÑÐ½ÐºÑÐ¸Ð¹ ANO6 Ð¿ÑÐ¸Ð²Ð¾Ð´ÑÑ Ðº ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ñ Ð¼Ð½Ð¾Ð¶ÐµÑÑÐ²Ð° Ð¿Ð°ÑÐ¾Ð»Ð¾Ð³Ð¸Ð¹. Ð¡ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¼ÐµÑÐ¾Ð´Ð° Ð»Ð¾ÐºÐ°Ð»ÑÐ½Ð¾Ð¹ ÑÐ¸ÐºÑÐ°ÑÐ¸Ð¸ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð° (patch-clamp) Ð¼Ñ ÑÐµÐ³Ð¸ÑÑÑÐ¸ÑÐ¾Ð²Ð°Ð»Ð¸ ÑÐ¾ÐºÐ¸ ÑÐµÑÐµÐ· Ð¾Ð´Ð¸Ð½Ð¾ÑÐ½ÑÐµ ÑÐ½Ð´Ð¾Ð³ÐµÐ½Ð½ÑÐµ ÐºÐ°Ð½Ð°Ð»Ñ ANO6 Ñ ÑÐµÐ»ÑÑ Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð¸Ð¾Ð½Ð¾Ð² ÐºÐ°Ð»ÑÑÐ¸Ñ Ð¿ÑÐ¸ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð¸ Ð½Ð°ÑÑÐ¶Ð½ÑÑ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸ÑÑ Ð½Ð° ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° ÐºÐ°Ð½Ð°Ð»Ð¾Ð². ÐÑ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð»Ð¸, ÑÑÐ¾ Ð²ÑÑÐ¾ÐºÐ°Ñ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ñ Ð¸Ð¾Ð½Ð¾Ð² ÐºÐ°Ð»ÑÑÐ¸Ñ Ð²Ð¾ Ð²Ð½ÐµÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¼ ÑÐ°ÑÑÐ²Ð¾ÑÐµ ÑÐ²ÐµÐ»Ð¸ÑÐ¸Ð²Ð°ÐµÑ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÑÐ½Ð´Ð¾Ð³ÐµÐ½Ð½ÑÑ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² ANO6; Ð¿ÑÐ¾Ð½Ð¸ÑÐ°ÐµÐ¼Ð¾ÑÑÑ ÑÐ½Ð´Ð¾Ð³ÐµÐ½Ð½ÑÑ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² ANO6 Ð¿Ð¾ Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ñ Ðº ÑÐ»Ð¾ÑÐ¸Ð´-Ð¸Ð¾Ð½Ð°Ð¼ Ð½Ðµ Ð·Ð°Ð²Ð¸ÑÐ¸Ñ Ð¾Ñ Ð²Ð½ÐµÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ Ð¸Ð¾Ð½Ð¾Ð² ÐºÐ°Ð»ÑÑÐ¸Ñ; ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ðµ Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ ÐºÐ°Ð»ÑÑÐ¸Ñ Ð¿ÑÐ¸Ð²Ð¾Ð´Ð¸Ñ Ðº Ð°ÐºÑÐ¸Ð²Ð°ÑÐ¸Ð¸ ÑÐ½Ð´Ð¾Ð³ÐµÐ½Ð½ÑÑ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² ANO6 Ñ Ð´Ð²Ð¾Ð¹Ð½Ð¾Ð¹ Ð°Ð¼Ð¿Ð»Ð¸ÑÑÐ´Ð¾Ð¹ ÑÐ¾ÐºÐ°; ÐºÐ¸Ð½ÐµÑÐ¸ÐºÐ° ÑÐ°Ð±Ð¾ÑÑ ÐºÐ°Ð½Ð°Ð»Ð° Ð·Ð°Ð²Ð¸ÑÐ¸Ñ Ð¾Ñ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð° Ð¿Ð»Ð°Ð·Ð¼Ð°ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ñ, Ð½Ð¾ Ð½Ðµ Ð¾Ñ Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ Ð¸Ð¾Ð½Ð¾Ð² ÐºÐ°Ð»ÑÑÐ¸Ñ. ÐÑÑÐ²Ð»ÐµÐ½Ð½ÑÐµ Ð½Ð°Ð¼Ð¸ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ñ ÑÐµÐ³ÑÐ»ÑÑÐ¸Ð¸ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² ANO6 Ð´ÐµÐ¼Ð¾Ð½ÑÑÑÐ¸ÑÑÑÑ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½ÑÐµ Ð¿ÑÑÐ¸ ÑÐ¾Ð½ÐºÐ¾Ð¹ Ð½Ð°ÑÑÑÐ¾Ð¹ÐºÐ¸ ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÐºÐ°Ð½Ð°Ð»Ð° Ð² Ð½Ð¾ÑÐ¼Ð°Ð»ÑÐ½ÑÑ ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ ÑÑÐ»Ð¾Ð²Ð¸ÑÑ Ð¸ Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ Ð¿Ð°ÑÐ¾ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ð¸.
description: Proteins of the anoctamine (ANO) family form calcium-activated chloride channels (CaCC). The most interesting member of the family is ANO6 (TMEM16F). ANO6 is a transmembrane protein with lipid scramblase and ion channel functions. ANO6 is involved in the modulation of plasma membrane area and regulation of transmembrane potential. Dysfunctions of ANO6 lead to many pathologies. Using patch-clamp method, we recorded currents through single endogenous ANO6 channels to study the effect of calcium ions at various intracellular and external concentrations on the electrophysiological properties of the channels. We showed that a high concentration of calcium ions in the extracellular solution increases the activity of endogenous ANO6 channels; the permeability of endogenous ANO6 channels to chloride ions does not depend on the extracellular concentration of calcium ions; an increase in intracellular calcium concentration leads to activation of endogenous ANO6 channels with double amplitude; the kinetics of the channel depends on the plasma membrane potential, but not on the intracellular concentration of calcium ions. The mechanisms we identified for regulating the activity of ANO6 channels demonstrate possible ways to fine-tune the functioning of the channel under normal physiological conditions and under pathophysiological conditions.