Additional file 1 of Dose-related adaptive reconstruction of DMN in isoflurane administration: a study in the rat
Creators: name: Guo, Fengru
name: Li, Yuqin
name: Jian, Zhaoxin
name: Cui, Yan
name: Gong, Wenhui
name: Li, Airui
name: Jing, Wei
name: Xu, Peng
name: Chen, Ke
name: Guo, Daqing
name: Yao, Dezhong
name: Xia, Yang
Publisher: name: figshare
Year: 2023
Subjects: subject: Biophysics
subject: Physical Sciences not elsewhere classified
subject: Genetics
subject: FOS: Biological sciences
subject: Neuroscience
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Information Systems not elsewhere classified

description: Additional file1: Figure S1. Original data QA, Quiet awake state. PrL, prelimbic cortex; OFC, orbitofrontal cortex; Cg, cingulate cortex; RSC, retrosplenial cortex; Hip, hippocampus; PPC, posterior parietal cortex; V2, secondary visual cortex; TeA, auditory/temporal association cortex. R, right, L, left. Figure S2. Histological tests were used to determine electrode positions [atlas adapted from (Paxinos and Watson, 2005)]. (a) Electrode position of PrL. (b) Electrode position of OFC. (c) Electrode position of CG. (d) Electrode position of Hip. STable 2. Vital parameters. under different isoflurane doses. Figure S3. Functional connectivity Functional connectivity under QA and three isoflurane-dose states in different frequency bands. (a-d) Network topology in the delta, theta, alpha, and beta bands in QA state, 0.75%, 1.25%, and 1.75%. PrL, prelimbic cortex; OFC, orbital cortex; Cg, cingulate cortex; Hip, hippocampus; PPC, posterior parietal cortex; TeA, auditory/temporal association cortex; V2, secondary visual cortex; RSC, retrosplenial cortex. n = 20. Figure S4. FCS of the DMN under QA and three isoflurane-dose states in the four frequency bands. The dots refer to the FCS values of each rat. ∗ indicates a significant difference between each state (QA, 0.75% isoflurane, 1.25% isoflurane, and 1.75% isoflurane) using one-way ANOVA with a Bonferroni post hoc test. The level of significance is p &lt; 0.05. n = 20. Figure S5. DMN topologies with the 20% smallest fluctuation FC in the DMN across different states are measured by fuzzy entropy in 1-30 Hz in different bands. (a–d) 20% of smallest the connections are based on fuzzy entropy of network topology in the delta, theta, alpha, and beta bands across QA, 0.75%, 1.25%, and 1.75% isoflurane-dose states. The blue lines indicate the top 20% largest variation of connections based on the fuzzy entropy of PLVs. The dot size indicates the degree of centrality of the DMN regions. n = 20. Figure S6. DMN topologies with the 20% largest fluctuation FC in the DMN across different states are measured by fuzzy entropy in 1-30 Hz. (a–d) 20% of the largest variations of the connections are based on fuzzy entropy of network topology in the delta and beta bands across QA, 0.75%, 1.25%, and 0.75% states. The red lines indicate the smallest 20% of connections based on the fuzzy entropy of PLVs. n = 20. Figure S7. 20% smallest and 20% largest fluctuation FC in the DMN across different states in the four frequency bands. (a–d) The FCS of the stable network topology. (e-h) The FCS of flexible network topology (large fluctuation). (a-d) The FCS of the flexible network topology. n = 20. STable 3. Simple linear regression between different isoflurane doses and FCS, PSD, and topological features.