Additional file 2: Figure S1. of The genome of newly classified Ochroconis mirabilis: Insights into fungal adaptation to different living conditions
Creators: name: Yew, Su
name: Chan, Chai
name: Kuan, Chee
name: Toh, Yue
name: Ngeow, Yun
name: Shiang Na
name: Lee, Kok
name: Chee-Choong Hoh
name: Wai-Yan Yee
name: Ng, Kee
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biochemistry
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Genetics
subject: Molecular Biology
subject: Pharmacology
subject: Evolutionary Biology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: FOS: Earth and related environmental sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: FOS: Health sciences
subject: Plant Biology
subject: 60506 Virology
subject: Computational Biology

description: KEGG map of styrene degradation. Genes annotated via KEGG are shaded. Z-phenylacetaldoxime degradation by nitrilase (EC 3.5.5.1), nitrile hydratase (EC 4.2.1.84) and amidase (EC 3.5.1.4). Although the phenylacetaldoxime dehydratase (EC 4.99.1.7) was not mapped, the gene was found in the genome. Figure S2. Alignment of putative phenylacetaldoxime dehydratase of O. mirabilis UM 578 (UM578_4049) with Bacillus sp. OxB-1 (P82604). Identical and similar residues are black and gray shaded respectively. The haem-containing dehydratase region is indicated by asterisk. Figure S3. Putative aldoxime-nitrile pathway gene cluster of UM 578. The phyenylacetaldoxime dehydratase (UM578_4049) and nitrilase (UM578_5050). The direction of transcription is indicated by the arrow for each gene. Figure S4. KEGG map of atrazine degradation. Genes annotated via KEGG are shaded. Cyanamide was degraded by cyanamide hydratase (EC 4.2.1.69) and urease (EC 35.1.5). Figure S5. Alignment of predicted metallopeptidase M14A of O. mirabilis UM 578 (UM578_1644). Alignment was carried out with metallopeptidase MeCPA from Metarhizium anisopliae (AAB68600) and TruMcpA from Trichophyton rubrum (ABW79919). Identical and similar residues are black and gray shaded, respectively. The zinc-binding residues are indicated by an asterisk. The active-site residues are indicated by circles. Conserved residues involved in substrate binding are indicated by solid triangles. The conserved Cys residues forming disulfide bridges are indicated by solid rhombus. Figure S6. Alignment of predicted serine carboxypeptidase of O.mirabilis UM 578 (UM578_13449). Alignment was carried out with TruSCPA from Trichophyton rubrum (AAS76667) and AfuCp1 from Aspergillus fumigatus (AAR91697). Identical and similar residues are black and gray shaded respectively. The consensus active residues are indicated by asterisk (Ser228, Asp439 and His497). Figure S7. Alignment of predicted leucine aminopeptidase (LAP) of O. mirabilis UM 578 (UM578_7056). Alignment was carried out with TruLAP1 from Trichophyton rubrum (AAS76670) and AfuLAP1 from Aspergillus fumigatus (AAR996058). Identical and similar residues are black and gray shaded respectively. The consensus binding sites for the first and the second Zn2+ ion binding sites are indicated in triangle (His180 and Asp265) and in rhombus (Glu238 and His347) respectively. The Asp199 is the residue bridging the two Zn2+ ions is indicated in circle. The active sites (Asp182 and Glu237) are indicated by asterisk. Figure S8. Alignment of predicted leucine aminopeptidase (LAP) of O.mirabilis UM 578 (UM578_5513). Alignment was carried out with TruLAP2 from Trichophyton rubrum (AAS76669) and AfuLAP2 from Aspergillus fumigatus (AAR96059). Identical and similar residues are black and gray shaded respectively. The consensus binding sites for the first and the second Zn2+ ion binding sites are indicated in triangle (His252 and Asp326) and in rhombus (Glu297 and His424) respectively. The Asp264 is the residue bridging the two Zn2+ ions is indicated in circle. The active sites (Asp254 and Glu296) are indicated in asterisk. Figure S9. Alignment of predicted dipeptidyl peptidase IV (DPPIV) of O. mirabilis UM 578 (UM578_9285). Alignment was carried out with TruDPPIV from Trichophyton rubrum (AAS76665) and AfuDPPIV from Aspergillus fumigatus (AAC34310). Identical and similar residues are black and gray shaded respectively. The catalytic triad is indicated in asterisk (Ser619, Asp 696, His731). Figure S10. Alignment of predicted dipeptidyl peptidase V (DPPV) of O. mirabilis UM 578 (UM578_9264). Alignment was carried out with TruDPPIV from Trichophyton rubrum (AAN03632) and AfuDPPIV from Aspergillus fumigatus (AAB67282). Identical and similar residues are black and gray shaded respectively. The catalytic triad is indicated in asterisk (Ser566, Asp647, His679). Figure S11. TMpred output of putative sulphite efflux pump (ssu1) in UM 578. The putative gene, UM578_9214 has ten membrane-spanning helixes and hydrophilic N- and C- termini. Figure S12. Putative melanin biosynthesis cluster in UM 578. The organisation and orientation of genes involved in melanin biosynthesis are similar to that of the reported melanin gene cluster in C. heterostrophus [GenBank: AAR90272] and A. brassicicola [GenBank: BAD22832]. The predicted genes encode polyketide synthase (UM578_2557), transcription factor Cmr1 (UM578_2558) and tetrahydroxynaphthalene reductase (UM578_2559). The direction of transcription is indicated by the arrow for each gene. Figure S13. Putative trichothecene biosynthesis cluster in UM 578. The 6751Â bp cluster encompasses the trichodiene synthase (UM578_3030) with two cytochrome P450 encoding genes (UM578_3031 and UM578_3032) and the trichothecene efflux pump (UM578_3033). The direction of transcription is indicated by the arrow for each gene. Figure S14. Putative gene organisation of mating type genes in UM 578. The neighbouring genes of alpha-domain containing gene (UM578_3656) encompass the homeodomain-containing protein (UM578_3655), DNA lyase APN2 (UM578_3657) and cytochrome C oxidase Vla Cox13 (UM578_3658). The direction of transcription is indicated by the arrow for each gene. Figure S15. Phylogenomic tree showing number of each node in expansion/ contraction analysis. The number of genes and P-value for UM 578 (node 24) and the internode (node 23) are shown in Additional file 1: Table S12. (PDF 1000 kb)