cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Mitochondrial DAMPs-dependent inflammasome activation during aging induces vascular smooth muscle cell dysfunction and aortic stiffness in low aerobic capacity rats
Creators: name: Canugovi, Chandrika
name: Stevenson, Mark D
name: Vendrov, Aleksandr E
name: Lozhkin, Andrey
name: Britton, Steven L
name: Koch, Lauren G
name: Runge, Marschall S
name: Madamanchi, Nageswara R
Publisher: name: OAE Publishing Inc.
Year: 2022
Subjects: Cardiovascular

description: Introduction: Low aerobic exercise capacity is an independent risk factor for cardiovascular disease (CVD) and a predictor of premature death. In combination with aging, low aerobic capacity lowers the threshold for CVD. Aim: Since low aerobic capacity and aging have been linked to mitochondrial oxidative stress and dysfunction, we investigated whether aged Low-Capacity Runner (LCR) rats (27 months) had vascular dysfunction compared to High-Capacity Runner (HCR) rats. Methods and Results: A significant decrease in aortic eNOS levels and vasodilation as well as an increase in aortic collagen and stiffness were observed in aged LCR rats compared to age and sex-matched HCR rats. There was a correlation between age-related vascular dysfunction and increased levels of ROS and DNA damage in aortas of LCR rats. Moreover, mitochondrial oxygen consumption, membrane potential, ATP levels, and mitophagy were lower in VSMCs of aged LCR rats. VSMCs from older LCR rats showed AIM2 inflammasome activation. VSMCs of young (4 months old) LCR rats treated with purified mitochondrial damage-associated molecular patterns (DAMP) recapitulated an inflammasome activation phenotype similar to that seen in aged rat VSMCs. Rapamycin, a potent immunosuppressant, induced mitophagy, stimulated electron transport chain activity, reduced inflammasome activity, mitochondrial ROS and DAMP levels in VSMCs from aged LCR rats. MitoTEMPO, a mitochondrial ROS scavenger, was similarly effective on VSMCs from aged rats. Conclusion: The findings suggest that impaired mitophagy and inflammasome activation in the vasculature under conditions of low aerobic exercise capacity during aging results in arterial dysfunction and aortic stiffness. In older adults with reduced aerobic capacity, mitochondrial antioxidants, mitophagy induction, and inflammasome inhibition may be effective therapeutic strategies for enhancing vascular health.
Differential games of partial information forward-backward doubly stochastic differential equations and applications
Creators: name: Hui, Eddie C. M.
name: Xiao, Hua
Publisher: name: arXiv
Year: 2011
Subjects: subject: Optimization and Control (math.OC)
subject: Systems and Control (eess.SY)
subject: FOS: Mathematics
subject: FOS: Electrical engineering, electronic engineering, information engineering
subject: 93E05, 90C39, 93E20

description: This paper is concerned with a new type of differential game problems of forwardbackward stochastic systems. There are three distinguishing features: Firstly, our game systems are forward-backward doubly stochastic differential equations, which is a class of more general game systems than other forward-backward stochastic game systems without doubly stochastic terms; Secondly, forward equations are directly related to backward equations at initial time, not terminal time; Thirdly, the admissible control is required to be adapted to a sub-information of the full information generated by the underlying Brownian motions. We give a necessary and a sufficient conditions for both an equilibrium point of nonzero-sum games and a saddle point of zero-sum games. Finally, we work out an example of linear-quadratic nonzero-sum differential games to illustrate the theoretical applications. Applying some stochastic filtering techniques, we obtain the explicit expression of the equilibrium point.
description: 29 pages
Stabilization Of Native & Functional Membrane Proteins: Calixar Approach
Creators: name: Mandon, Elodie
Publisher: name: Zenodo
Year: 2015
Subjects: Native functional membrane proteins
stability
Membrane protein production

description: Membrane proteins represents up to 70% of therapeutic targets that are involved in different key cellular processes and signaling events. Surprisingly, less than 1% of protein structures in the Protein Data Bank correspond to membrane proteins. Those targets are very often instable which represents a bottleneck for their production in homogenous and stable state suitable for structural studies, antibody development and HTS ligand screening. CALIXAR has developed an innovative approach consisting on native isolation of therapeutic targets from the expression, solubilization/stabilisation, and purification to functional as well as structural characterization. Instead of modifying the protein (by truncations and mutations) to adapt to the environment, we modify the chemical environment to adapt to the protein, using innovative proprietary detergents/ surfactants compounds and combination of compounds to help solubilize and stabilize in the same time. The starting material can be either endogenous (Organs, Primary cells, bacteria, and viruses), or recombinant (E.coli, Yeast, insect cells, CHO and HEK cells). Here we will describe examples of highly druggable targets produced in their most native state without any single mutation, truncation or fusion and that could exhibit striking thermo-stability increase and homogeneity improvement, while keeping their functional features. Indeed, more than 30C thermostability improvement was obtained for the human transporter BCRP while keeping its native sequence and modifying only the chemical environment. We show striking stability improvement of 7TM proteins: bacteriorhodopsin and wild type and non-truncated adenosine receptor for which crystals could be obtained and antibodies were derived. Finally, conditions of functional stabilization of the trimer of HIV envelop protein were also found. This will pave the way to future structural investigations and conformational antibody development of native HIV envelop.
The Carnegie Hubble Program
Creators: name: Freedman, Wendy L.
name: Madore, Barry F.
name: Scowcroft, Vicky
name: Monson, Andy
name: Persson, S. E.
name: Seibert, Mark
name: Rigby, Jane
name: Sturch, Laura
name: Stetson, Peter
Publisher: name: arXiv
Year: 2011
Subjects: subject: Cosmology and Nongalactic Astrophysics (astro-ph.CO)
subject: FOS: Physical sciences

description: We present an overview of and preliminary results from an ongoing comprehensive program that has a goal of determining the Hubble constant to a systematic accuracy of 2%. As part of this program, we are currently obtaining 3.6 micron data using the Infrared Array Camera (IRAC) on Spitzer, and the program is designed to include JWST in the future. We demonstrate that the mid-infrared period-luminosity relation for Cepheids at 3.6 microns is the most accurate means of measuring Cepheid distances to date. At 3.6 microns, it is possible to minimize the known remaining systematic uncertainties in the Cepheid extragalactic distance scale. We discuss the advantages of 3.6 micron observations in minimizing systematic effects in the Cepheid calibration of the Hubble constant including the absolute zero point, extinction corrections, and the effects of metallicity on the colors and magnitudes of Cepheids. We are undertaking three independent tests of the sensitivity of the mid-IR Cepheid Leavitt Law to metallicity, which when combined will allow a robust constraint on the effect. Finally, we are providing a new mid-IR Tully-Fisher relation for spiral galaxies.
In Two Minds: Arts and Science Differences at Sixth Form Level, 1980
Creators: name: Bamber, J. H.
name: Corbett, W. D.
name: Bill, J. M.
name: Boyd, F. E.
Publisher: name: UK Data Service
Year: 1982

description: The purpose of this study was to identify differences between arts and science specialists at sixth form level on a range of cognitive, personality and attitudinal measures.
An Assessment of toxic waste control policy in Alberta and Ontario: the PCB example
Creators: name: O'Keefe, Cornelius J.
Publisher: name: Environmental Design
Year: 1990
Subjects: subject: T 55.3 H3 O53 1990
subject: Hazardous wastes - Law and legislation - Alberta
subject: Hazardous wastes - Law and legislation - Ontario
subject: Polychlorinated biphenyls

description: Bibliography: p. 284-307.
Unravelling the novel Higgs mechanism in (2+1)d Chern-Simons theories
Creators: name: Mukhi, Sunil
Publisher: name: arXiv
Year: 2011
Subjects: subject: High Energy Physics - Theory (hep-th)
subject: Strongly Correlated Electrons (cond-mat.str-el)
subject: General Relativity and Quantum Cosmology (gr-qc)
subject: FOS: Physical sciences

description: Chern-Simons gauge theories in 2+1 dimensions with multiple gauge fields exhibit novel properties that are analysed here in some detail. A striking feature is the possibility of a non-propagating Chern-Simons field acquiring a massless propagating mode via a Higgs mechanism. This novel Higgs mechanism, originally discovered in the context of M-theory, is studied here without reference to M-theory or supersymmetry. It is revealed as a variant of topological mass generation and shown to arise only when Chern-Simons and mass matrices are not simultaneously diagonalisable. Sufficient conditions are found for it to occur. It is speculated that some analogue of the NHM could occur in theories of condensed-matter systems similar to those exhibiting the fractional quantum Hall effect, as well as in 2+1 dimensional gravity.
description: 28 pages
Coronary heart disease among the Dariusleut Hutterites of Alberta
Creators: name: Brunt, J. Howard
Publisher: name: University of Calgary
Year: 1990
Subjects: subject: RC 685 C6 B78 1989
subject: Coronary heart disease - Alberta
subject: Hutterite Brethern - Alberta - Diseases

description: Bibliography: p. 171-191.
Trade Unionism among Married Women in Relation to Their Family Roles, 1979
Creators: name: Cunnison, S.
Publisher: name: UK Data Service
Year: 1982

description: The purpose of this study was:
 1) to examine similarities and differences between men and women with regard to union involvement and perceptions of objectives in career and promotion, and the relation of these to family responsibilities;
 2) to examine similarities and differences between women members of the National Union of Teachers and National Association of Schoolmasters/Union of Women Teachers.
Anisotropic magnetoresistance of bulk carbon nanotube sheets
Creators: name: Cimpoiasu, E.
name: Levin, G. A.
name: White, B.
name: Lashmore, D.
Publisher: name: arXiv
Year: 2012
Subjects: subject: Mesoscale and Nanoscale Physics (cond-mat.mes-hall)
subject: FOS: Physical sciences

description: We have measured the magnetoresistance of stretched sheets of carbon nanotubes in temperatures ranging from 2 K to 300 K and in magnetic fields up to 9 T, oriented either perpendicular or parallel to the plane of the sheets. The samples have been partially aligned by post-fabrication stretching, such that the direction of stretching was either parallel or perpendicular to the direction of applied electric current. We have observed large differences between the magnetoresistance measured under the two field orientations, most pronounced at the lowest temperatures, highest fields, and for the laterally-aligned sample. Treatment of the sheets with nitric acid affects this anisotropy. We analyzed the results within the theoretical framework of weak and strong localization and concluded that the anisotropy bears the mark of a more unusual phenomenon, possibly magnetically-induced mechanical strain.
description: 34 pages, 10 figures
Computational Methods In Gpcr Mechanism Study And Drug Discovery
Creators: name: Yuan, Shuguang
Publisher: name: Zenodo
Year: 2015
Subjects: GPCR
Computational Structural Biology
Activation Mechanism
Water Channel

description: More than 800 human GPCRs allow the selective detection of extracellular signals as diverse as photons, odorants, flavors, nucleotides, hormones, neurotransmitters ? revealing GPCRs fundamental role in signal transduction. As they regulate many central physiological processes and are thus implicated in many diseases, GPCRs are among the most important targets for modern medicines. In spite of this medical importance and the recent progress in elucidating the 3D structures of various GPCRs, central questions how these receptors recognize extracellular chemical signals and transfer them across the cellular membrane to finally evoke an intracellular response are largely unresolved at a molecular level, mainly because the different steps during signal transmission are not directly accessible by experiments. In this context we are concentrating on central questions of GPCR mediated cellular signalling using computer based molecular dynamics simulations and structural modelling. Our work revealed for the first time, in atomic detail, the entire process of transmembrane signalling of various GPCRs: we found that ligand binding induces a series of conformational changes within a GPCR which opened a gate inside the receptor for water molecules entering the internal region of the receptor and subsequently driving conformational switches within the receptor which finally led to the activation of a G protein on the intracellular side of the receptor. With the uncovered principle, 17 out 25 newly screened compounds were proved to be active for odorant receptors. The discovery of these internal water channels  paved the way for novel approaches in drug development.
Ultrasensitive Measurement Of Mutant Huntingtin In Human Csf And Rodent Samples
Creators: name: Fodale, Valentina
Publisher: name: Zenodo
Year: 2015
Subjects: ultra-sensitive measurement
CSF
huntingtin
huntington disease

description: Huntington disease (HD) is a neurodegenerative disorder caused by a genetic defect in Huntingtin gene (HTT) that leads to the expression of an expanded poly-glutamine protein. HD has a broad impact on a person's functional abilities due to progressive motor dysfunction, cognitive decline, and psychiatric disturbance, probably caused by both neuronal dysfunction and neuronal cell death. New strategies for drug discovery and new therapeutic approaches are now leading to slow the progression of HD; one of these promising strategies consists in reducing the mutant protein level through its direct reduction (gene silencing) or increasing its clearance (compound treatment). In this framework mutant HTT protein can be a biomarker for treatment efficacy and disease progression, raising the necessity to develop highly sensitive immunoassays to quantify its level in biological fluids. The Single Molecule Counting (SMC?) technology, powered by Singulex?, enabled the ultrasensitive measurement of mutant HTT at levels previously undetectable in human Cerebrospinal fluid (CSF). The immunoassay was developed using the 2B7 and the MW1 antibodies that recognize the N-terminal region and the poly-Q trait of the protein respectively and was successfully applied to human and murine derived fluids and murine tissues. Here is presented a summary of results obtained from the assay validation process, including the evaluation of calibration curve performance, specificity, matrix effect (spike recovery, parallelism and dilution linearity), selectivity, accuracy, precision and stability
Pulmonary inflammation and immune responses to the namatode nippostrongylus brasiliensis
Creators: name: Ramaswamy, Kalyanasundaram
Publisher: name: University of Calgary
Year: 1990
Subjects: subject: QL 391 N4 R35 1990
subject: Nippostrongylus brasiliensis
subject: Helminthiasis

description: Bibliography: p. 294-358.
The Division of housework between dual earner couples: an exploratory study
Creators: name: Robertson, Shan A.
Publisher: name: University of Calgary
Year: 1990
Subjects: subject: HD 6060.6 R63 1990
subject: Home economics
subject: Wives - Attitudes
subject: Husbands - Attitudes
subject: Sexual division of labor

description: Bibliography: p. 79-84.
Anomalous light shift through quantum jumps in quasi-resonant Rayleigh scattering
Creators: name: Norris, D. G.
name: Cimmarusti, A. D.
name: Orozco, L. A.
name: Barberis-Blostein, P.
name: Carmichael, H. J.
Publisher: name: arXiv
Year: 2012
Subjects: subject: Quantum Physics (quant-ph)
subject: Atomic Physics (physics.atom-ph)
subject: FOS: Physical sciences

description: An anomalous light shift in the precession of a ground-state Zeeman coherence is observed: the Larmor frequency increases with the strength of a drive that is blue (red) detuned from a transition out of the lower (upper) energy level. Our measurements are made on Rb 85 atoms traversing an optical cavity containing a few photons; shifts as large as 1% per photon are recorded. The anomalous shift arises from an accumulation of phase driven by quantum jumps. It is stochastic and accompanied by broadening.
description: 5 pages, 4 figures
Microscale Hydrogel Patterning To Manipulate Stem Cell Fate
Creators: name: Kolb, Laura
name: Mosiewicz, Katarzyna A.
name: Andr? J. Van Der Vlies
name: Nikolche Gjorevski
name: Lutolf, Matthias P.
Publisher: name: Zenodo
Year: 2015

description: Stem cell behavior is strongly influenced by the physical and chemical cues originating from the extracellular matrix (ECM). In vivo, ECM signals are displayed in a spatiotemporally complex fashion. Current in vitro models to study the role of ECM signals in regulating stem cell behavior are rather limited in recapitulating this microenvironmental complexity, as they are mostly static and homogeneous. In order to achieve a dynamic control of the physical and biochemical properties of a hydrogel network, we here combined two crosslinking reaction schemes with photochemistry in order to control hydrogel stiffness in space, time and intensity.
First, thiol moieties of one of the reactive poly(ethylene glycol) (PEG) macromers undergoing crosslinking into a hydrogel network via Michael-type addition were equipped with caging groups. This prevented their reaction with vinyl sulfone groups on the termini of the complementary PEG macromers. Thus, the crosslinking density of the hydrogel network could be controlled by uncaging with light, directly translating into differential hydrogel stiffness. Using this approach, user-defined two-dimensional stiffness patterns between 3-8 kPa (E modulus) were obtained and shown to influence the directional migration of mesenchymal stem/progenitor cells in a Young?s modulus-dependent manner with constant gradient strength. Cell migration was overall more directed on the photo-patterned substrate compared to the non-patterned region, with a bias towards the stiffer region above 5 kPa.
Second, we present an approach based on light-activated enzymatic crosslinking by which the biophysical properties of synthetic hydrogels can be modulated in 3D. A peptide substrate of the activated transglutaminase Factor XIII (FXIIIa), a key ECM crosslinking enzyme, was rendered photosensitive by masking its active site with a photolabile cage group. Covalent incorporation of this caged FXIIIa substrate together with the corresponding acceptor enzyme substrate into PEG hydrogels and subsequent laser-scanning lithography affords highly localized additional crosslinking. By encapsulation of intestinal stem cell-derived organoids followed by the photopatterning of precise three-dimensional stiffness areas we hope to be able to direct their self-organization.
These cytocompatible and versatile patterning concepts are highly promising for the manipulation of cell fate in stem cell research, disease modeling, and regenerative medicine.
A relativistic iron emission line from the neutron star low-mass X-ray binary GX 3+1
Creators: name: Piraino, S.
name: Santangelo, A.
name: Kaaret, P.
name: Mück, B.
name: D'Aì, A.
name: DI Salvo, T.
name: Iaria, R.
name: Robba, N.
name: Burderi, L.
name: Egron, E.
Publisher: name: arXiv
Year: 2012
Subjects: subject: High Energy Astrophysical Phenomena (astro-ph.HE)
subject: FOS: Physical sciences

description: We present the results of a spectroscopic study of the Fe Kα emission of the persistent neutron-star atoll low-mass X-ray binary and type I X-ray burster GX 3+1 with the EPIC-PN on board XMM-Newton. The source shows a flux modulation over several years and we observed it during its fainter phase, which corresponds to an X-ray luminosity of Lx~10^37 ergs/s. When fitted with a two-component model, the X-ray spectrum shows broad residuals at \sim6-7 keV that can be ascribed to an iron Kα fluorescence line. In addition, lower energy features are observed at \sim3.3 keV, \sim3.9 keV and might originate from Ar XVIII and Ca XIX. The broad iron line feature is well fitted with a relativistically smeared profile. This result is robust against possible systematics caused by instrumental pile-up effects. Assuming that the line is produced by reflection from the inner accretion disk, we infer an inner disk radius of \sim25 Rg and a disk inclination of 35° < i < 44°.
description: 4 pages, 3 figures Accepted for publication in Astronomy and Astrophysics
The Modified Trebble-Bishnoi equation of state
Creators: name: Salim, Paul Hartawan
Publisher: name: Engineering
Year: 1990
Subjects: subject: QC 173.4 E65 S34 1990
subject: Equations of state

description: Bibliography: p. 122-127.
description: An inconsistency in thermodynamic property prediction using the Trebble- Bishnoi equation of state was recently identified by scientists at the Shell research center in Amsterdam. Specifically, it was found that the Trebble-Bishnoi equation of state predicted negative values of isochoric heat capacity and imaginary values for speed of sound at extremely low temperatures. Subsequent investigation has led to the conclusion that any temperature dependence whatsoever in the covolume term of van der Waals type equations will re~ult in anomalies in the predicted thermodynamic properties of pure fluids at extreme conditions. This work presents a discussion of the implication of temperature dependence in the Trebble-Bishnoi equation of state parameters and describes a modification to the equation in which temperature dependence is completely removed from the covolume term. By altering the temperature dependence in the attractive parameter, it is possible to correlate liquid-vapor phase behaviour with the modified equation more accurately than with the original equation, and the inconsistency has been corrected. The _modified equation was examined further through the calculation of eight thermodynamic properties in liquid-vapor regions over a very broad range of temperature and pressure. Predictions of second virial coefficients, internal energy, enthalpy, entropy, isobaric and isochoric heat capacities, speed of sound, and Joule-Thomson coefficients were compared to experimental data and to properties calculated using the Peng-Robinson equation of state. Results from the modified equation are of reasonable accuracy and are comparable to those obtained from the Peng-Robinson equation. No thermodynamic anomalies were detected for either equation of state apart from the fact that an infinite value for isochoric heat capacity is not predicted at the critical point. Having developed a satisfactory model for describing liquid-vapor regions, it was desirable to extend the equation of state to pure solids so that consistent calculation of solid-vapor equilibria could also be achieved. Based on correct thermophysical constraints and utilizing solid heat capacity, solid density and heat of sublimation at the triple point, a methodology is presented for extending the use of the equation of state to pure solids. The methodology is tested through the predictions of PVT behaviour and isobaric heat capacities for solids from their triple points to very low reduced temperatures. It is found that there are no negative heat capacity predictions and that an equation of state with at least four parameters is required to provide good prediction of solid-vapor phase behavior. Also discussed is the estimation of binary interaction parameters for binary solid-supercritical fluid systems using classical equation of state mixing rules and a common flash algorithm.
Supplemental Material of Vivienne: Relational Verification of Cryptographic Implementations in WebAssembly
Creators: name: Tsoupidi, Rodothea Myrsini
name: Balliu, Musard
name: Baudry, Benoit
Publisher: name: Zenodo
Year: 2021

description: Supplemental material for the paper "Vivienne: Relational Verification of Cryptographic Implementations in WebAssembly".
Jayce Technology To Decipher Receptor Biology And Pharmacology
Creators: name: Kiry, Manon
Publisher: name: Zenodo
Year: 2015
Subjects: GPCR
Biased signaling
Luminescence
TagLite
Real-time

description: Traditional pharmacology posits that a ligand can be either classified as an agonist (full or partial), antagonist or inverse agonist through a specific receptor subtype, and that this characteristic will be consistent with all effector (second messenger) systems coupled to that receptor. While this dogma has been the backbone of ligand-receptor interactions for decades, recent data indicates that this classic definition of ligand-protein associations does not hold true for a number of compounds; such compounds may be termed as mixed agonists-antagonists. Functional selectivity posits that a ligand may inherently produce a mix of the classic characteristics through a single receptor isoform depending on the effector pathway coupled to that receptor. For instance, a ligand cannot easily be classified as an agonist or antagonist, because it can combine both attribute, depending on its preferred signal transduction pathways. Thus, such ligands must instead be classified on the basis of their individual effects in the cell, instead of being either an agonist or antagonist to a receptor. Actelion?s JAYCE platform generates proprietary biological information delineating beneficial and adverse signaling pathways at specific receptors, and identifies ligands that activate only the pathways associated with therapeutic benefit. The platform is a purpose-built set of integrated tools and techniques including diverse proprietary assays of GPCR function and regulation , which can predict therapeutic index and offer insights into G protein and b-arrestin-mediated mechanisms associated with pharmacological responses. These assays are aligned with biological signaling information obtained by dissecting GPCR/7TM signal transduction networks with real-time measurement. The JAYCE platform is an effective and efficient means of discovering meaningfully differentiated biased ligands targeting important clinical needs.
Astronomical Sky Quality Near Eureka, in the Canadian High Arctic
Creators: name: Steinbring, Eric
name: Ward, William
name: Drummond, James R.
Publisher: name: arXiv
Year: 2011
Subjects: subject: Instrumentation and Methods for Astrophysics (astro-ph.IM)
subject: FOS: Physical sciences

description: Nighttime visible-light sky brightness and transparency are reported for the Polar Environment Research Laboratory (PEARL), located on a 610-m high ridge near the Eureka research station, on Ellesmere Island, Canada. Photometry of Polaris obtained in V band with the PEARL All Sky Imager (PASI) over two winters is supported by standard meteorological measurements and visual estimates of sky conditions from sea level. These data show that during the period of the study, October through March of 2008/09 and 2009/10, the sky near zenith had a mean surface brightness of 19.7 mag/square-arcsec when the sun was more than 12 deg below the horizon, reaching 20.7 mag/square-arcsec during astronomical darkness with no moon. Skies were without thick cloud and potentially usable for astronomy 86% of the time (extinction <2 mag). Up to 68% of the time was spectroscopic (<0.5 mag), attenuated by ice crystals, or clear with stable atmospheric transparency. Those conditions can persist for over 100 hours at a time. Further analysis suggests the sky was entirely free of ice crystals (truly photometric) 48+/-3% of the time at PEARL in winter, and that a higher elevation location nearby may be better.
description: 14 pages, 1 table, 11 figures; accepted for publication in PASP
Nuclear medium effects in $ν(\barν)$-nucleus deep inelastic scattering
Creators: name: Haider, H.
name: Simo, I. Ruiz
name: Athar, M. Sajjad
name: Vacas, M. J. Vicente
Publisher: name: arXiv
Year: 2011
Subjects: subject: Nuclear Theory (nucl-th)
subject: FOS: Physical sciences

description: We study the nuclear medium effects in the weak structure functions $F_2(x,Q^2)$ and $F_3(x,Q^2)$ in the deep inelastic neutrino/antineutrino reactions in nuclei. We use a theoretical model for the nuclear spectral functions which incorporates the conventional nuclear effects, such as Fermi motion, binding and nucleon correlations. We also consider the pion and rho meson cloud contributions calculated from a microscopic model for meson-nucleus self-energies. The calculations have been performed using relativistic nuclear spectral functions which include nucleon correlations. Our results are compared with the experimental data of NuTeV and CDHSW.
description: 24 pages, 14 figures
Metagenomics And Clinical Benchmarking With Low Coverage Oxford Nanopore Data
Creators: name: Alexander, Noah
Publisher: name: Zenodo
Year: 2015
Subjects: Nanopore

description: We have sequenced an equimolar mixture of six bacterial genomes of interest (Pseudomonas fluorescens, Escherichia coli, Halobacillus halophilus, Pseudoalteromonas haloplanktis, Staphylococcus epidermidis, and Micrococcus luteus) at low coverage using the oxford nanopore minION. We checked the utility of these data for metagenomics work using classification tools BLAST and Kraken. We have also sequenced an Enterobacter cloacae sample at low coverage (1.4X coverage of 2D data) to begin benchmarking with a sample containing known genetic material with an available reference genome with the same algorithms that were used with the bacterial pool. This dataset remains very low-coverage but does provide more coverage than the bacterial pool dataset affords each constituent sample. We have also sequenced the Genome in a Bottle HG-002 human genome reference material sample, one of the most deeply sequenced samples in existence, in order to assess the Viability of current nanopore methods for clinical sequencing work involving human genetic material.
Increased Production Of Antioxidants In The Presence Of Cadmium By Promicromonospora Sp. Utmc2243
Creators: name: Mirjalili, Meisam
Publisher: name: Zenodo
Year: 2015
Subjects: Actinomycetes
antioxidant
Promicromonospora
heavy metal resistance

description:  Reactive free radicals are detrimental agents which have various side effects cause many diseases such as cancer, atherosclerosis, etc. They also play an important role in deterioration of food. Therefore, antioxidant compounds with high performance are still demanding. Promicromonospora sp. UTMC 2243 is a rare actinomycete which was used in this experiment for considering its ability to produce antioxidants. In Previous studies resistance and removal of heavy metals has been shown by Promicromonospora sp. UTMC 2243. In this study increased production of antioxidants in the presence of cadmium ions was evaluated. The ISP2 media with different concentrations of cadmium chloride (0, 4.6 and 9.2 mM.5% v/v) was used to ferment the Promicromonospora UTMC2243. After fermentation, extraction with solvent was done to obtain crude extracts of each medium. All experiments were carried out in triplicate. Different concentrations of obtained extracts were used for free radical scavenging activity. Volumes of 10 ?l of various concentrations of obtained extracts (solutions with 100, 200 and 300 ?g of extract/mL of methanol) and Ascorbic acid and BHT (solution with 10, 20 , 30 ?g/ml) were mixed with 200 ?l of methanolic solution containing DPPH radicals (0.005%w/v). Each mixture was shaken vigorously and kept in a dark place for half hour and afterward the absorbance was read against a blank at 545nm. Inhibition of free radical DPPH in percent (I %) was calculated.  The extract concentration providing 50% inhibition (EC50) was calculated from the graph of scavenging effect against extracts concentration. The results showed that, secondary metabolites produced in media with high concentration of cd have more antioxidant activity. Even at the sub-MIC concentrations of cadmium chloride, antioxidants production were increased excessively which was comparable with Ascorbic acid and BHT. Metabolites produce in absence, 4.6 mM and 9.2 mM of cadmium chloride ha  EC 70-80 % showed 85-95% scavenging activity compared to 45% and 55% for Ascorbic acid and BHT respectively. It can be concluded that growing the Promicromonospora sp. UTMC 2243 under the metal stress can enhance the amount of the antioxidants which are produced by that. This phenomenon can be used in mass production of the antioxidant compound(s) that is consequently required for their structure elucidation.
A Predicted Chemo-Polypharmacophoric Agent Comprising (Propeptide-Fc)/Mgf Peptide Mimicking Interactive Of High Free Binding Energy Properties Towards Wnt7A/Fzd7 Signalling Akt/Mtor Anabolic Growth Igf-I/Pi3K/Akt -I/Mapk/Erk Pathways.
Creators: name: Grigoriadis, Ioannis
Publisher: name: Zenodo
Year: 2015
Subjects: A predicted chemo-polypharmacophoric agent comprising (Propeptide-Fc)/MGF peptide mimicking interactive of high free binding energy properties towards Wnt7a/Fzd7 signalling Akt/mTOR anabolic growth IGF-I/PI3K/Akt -I/MAPK/ERK pathways.

description: The insulin-like growth factor-I (IGF-I) is a key regulator of skeletal muscle growth in vertebrates, promoting mitogenic and anabolic effects through the activation of the MAPK/ERK and the PI3K/Akt signaling pathways. Also, these results show that there is a time-dependent regulation of IGF-I plasma levels and its signaling pathways in muscle. The insulin-like growth factor-I (IGF-I) is a key regulatory hormone that controls growth in vertebrates. Particularly, skeletal muscle growth is strongly stimulated by this hormone. IGFI stimulates both proliferation and differentiation of myoblasts, as well as promoting myotube hypertrophy in vitro and in vivo. The mitogenic and anabolic effects of IGF-I on muscle cells are mediated through specific binding with the IGF-I receptor (IGF-IR). This ligand-receptor interaction promotes the activation of two major intracellular signaling pathways, the mitogen-activated protein kinases (MAPKs), specifically the extracellular signal-regulated kinase (ERK), and the phosphatidylinositol 3 kinase (PI3K)/Akt. The MAPK (RAF/MEK/ERK) is a key signaling pathway in skeletal muscle, where its activation is absolutely indispensable for muscle cell proliferation. Biologically active polypeptides derived from the E domain that forms the C-terminus of the insulin-like growth factor I (IGF-I) splice variant known as mechano growth factor which have been demonstrated neuroprotective and cardioprotective properties, as well as the ability to increase the strength of normal and dystrophic skeletal muscle. Ligands selected from phage-displayed random peptide libraries tend to be directed to biologically relevant sites on the surface of the target protein. Protein-peptide interactions form the basis of many cellular processes. Consequently, peptides derived from library screenings often modulate the target protein's activity in vitro and in vivo and can be used as lead compounds in drug design and as alternatives to antibodies for target validation in both genomics and drug discovery. In this research and science project we for the first time a predicted chemo-polypharmacophoric agent comprising (Propeptide-Fc)/MGF peptide mimicking properties for the possible increasement of the Muscle Mass Fiber Size towards Wnt7a/Fzd7 Signalling to the Akt/mTOR Anabolic Growth IGF-I/PI3K/Akt -I/MAPK/ERK pathways utilising (Propeptide-Fc)/MGF phage-displayed random peptide libraries through a KNIME-RDkit-CDK clustering pipeline.
Hippocampal formation theta field and cell activities are controlled by cholinergic and GABAergic
Creators: name: Smythe, James William
Publisher: name: University of Calgary
Year: 1990
Subjects: subject: QP 383.25 S59 1990
subject: Theta rhythm
subject: Hippocampus (Brain)

description: Bibliography: p. 253-270.
description: offsite
Survey on the Condition of London's Housing, 1967
Creators: name: Greater London Council
Publisher: name: UK Data Service
Year: 1982

description: To provide information required in the preparation of the Greater London Development Plan. Estimates of the future population of London were to be based on the stock of dwellings that would exist in London. It was therefore necessary to know more about the condition and useful life of existing housing. At the same time, if occupancy rates were to be projected accurately, more needed to be known about the pressure for homes from hidden households (those living as part of another household because they cannot find separate accomodation).
Meiotic segregation of sperm chromosomes in two human reciprocal translocation heterozygotes
Creators: name: Spriggs, Elizabeth L.
Publisher: name: University of Calgary
Year: 1990
Subjects: subject: QH 605.3 S67 1990
subject: Meiosis
subject: Translocation (Genetics)
subject: Spermatozoa - Analysis

description: Bibliography: p. 112-120.
Real and complex supersymmetric d=1 sigma models with torsions
Creators: name: Fedoruk, S. A.
name: Ivanov, E. A.
name: Smilga, A. V.
Publisher: name: arXiv
Year: 2012
Subjects: subject: High Energy Physics - Theory (hep-th)
subject: Mathematical Physics (math-ph)
subject: FOS: Physical sciences

description: We derive and discuss, at both the classical and the quantum levels, generalized N = 2 supersymmetric quantum mechanical sigma models describing the motion over an arbitrary real or an arbitrary complex manifold with extra torsions. We analyze the relevant vacuum states to make explicit the fact that their number is not affected by adding the torsion terms.
description: 25 pages
A Mechanistic In Silico Molecular Recognized Approach For The Ligand Based Generation Of A Dual N-Formyl-Met-Leu-Phe (Fmlp), And Mmk-1Peptide Mimetic Hyper-Agonist Fmlp Targeted Receptor Against The Pge2 Ep4 Pathway Chemotherapy-Induced Alopecia.
Creators: name: Grigoriadis, Ioannis
Publisher: name: Zenodo
Year: 2015
Subjects: ligand based generation
dual N-formyl-Met-Leu-Phe (fMLP)
MMK-1peptide mimetic hyper-agonist
fMLP targeted receptor
PGE2 EP4 pathway chemotherapy-induced alopecia

description: It has been shown that the Oral administration for 6 days of 100 mg/kg MMK-1, of an agonist peptide selective for the FPRL1 receptor, suppressed alopecia induced by the anticancer drug etoposide in neonatal rats. However, the anti-alopecia effect of orally administered MMK-1 was inhibited by indomethacin, an inhibitor of cyclooxygenase (COX), or AH-23848B, an antagonist of the EP4 receptor for prostaglandin (PG) E2, suggesting involvement of PGE2 release and the EP4 receptor in the oral MMK-1 anti-alopecia mechanism. The anti-alopecia effect of orally administered MMK-1 was also blocked by an inhibitor of nuclear factor-kappaB (NF-kappaB), pyrrolidine dithiocarbamate, suggesting that the oral anti-alopecia effect of MMK-1 may be mediated by activation of NF-kappaB. These results suggested that MMK-1 bound to FPRL1 receptor might suppress etoposide-induced apoptosis of hair follicle cells and alopecia by way of PGE2 release and NF-kappaB activation. Previously, it has also been found that an intraperitoneally administered chemotactic peptide, N-formyl-Met-Leu-Phe (fMLP), and MMK-1, a selective agonist of formyl peptide receptor-like 1 (FPRL1) receptor, the low affinity subtype of the fMLP receptor, prevented the alopecia in neonatal rats induced by the anticancer agent etoposide. The anti-alopecia effect of fMLP was not inhibited at all by Boc-FLFLF, a selective antagonist of formylpeptide receptor (FPR), which is the high affinity subtype of the receptor, but it was partly inhibited by Trp-Arg-Trp-Trp-Trp-Trp-NH(2) (WRW(4)), an antagonist of FPRL1 receptor. The anti-alopecia effects of fMLP and MMK-1 were also inhibited by Lys-D-Pro-Thr (K(D)PT) and pyrrolidine dithiocarbamate, which are inhibitors of interleukin-1 (IL-1) and nuclear factor-kappaB (NF-kappaB) respectively. Computational methods utilizing the structural and functional information help to understand specific molecular recognition events between the target biomolecule and candidate hits and make it possible to design improved lead molecules for the target. Here we represents a massive on-going scientific endeavor to provide a freely accessible state of the art software suite for protein and DNA targeted lead molecule discovery. by resulting in a Mechanistic in silico molecular recognized approach for the ligand based generation of a dual N-formyl-Met-Leu-Phe (fMLP), and MMK-1peptide mimetic agonists formyl-peptide hyper-agonist interactive receptors against chemotherapy-induced alopecia.
Use of Television Equipment in Scottish Secondary Schools, 1980
Creators: name: Lawrie, N. L.
name: Hessett, C.
name: Waters, C. D. J.
Publisher: name: UK Data Service
Year: 1982

description: To collect data from schools about television equipment in schools, its use, organisational arrangements for its use, movement of pupils and equipment occurring in the course of use, the extent of software (in the form of programs on video cassette) held in schools, and about relevant aspects of the schools themselves, viz. roll, staffing and school capacity, with a view to finding the effect of a range of quantitative factors on use.
Matrix Product States with long-range Localizable Entanglement
Creators: name: Wahl, Thorsten B.
name: Perez-Garcia, David
name: Cirac, J. Ignacio
Publisher: name: arXiv
Year: 2012
Subjects: subject: Quantum Physics (quant-ph)
subject: Other Condensed Matter (cond-mat.other)
subject: FOS: Physical sciences

description: We derive a criterion to determine when a translationally invariant matrix product state (MPS) has long-range localizable entanglement, where that quantity remains finite in the thermodynamic limit. We give examples fulfilling this criterion and eventually use it to obtain all such MPS with bond dimension 2 and 3.
description: 8 pages, 1 figure
An In Silico Chemoproteomic Prediction-Scan For The Generation Of A Tyrosinase Aa95-104Fmgfncgnck Antigenic Patternlfa-3/Igg Fusion Polypeptide Ilealaargargpheleuoh (Kinetensin) Mimetic Pharmacophore On Conserved Vitiligo Post-Trancripts Domains.
Creators: name: Grigoriadis, Ioannis
Publisher: name: Zenodo
Year: 2015
Subjects: An in silico chemoproteomic prediction-scan
generation of a tyrosinase aa95-104FMGFNCGNCK antigenic pattern
LFA-3/IgG fusion polypeptide
IleAlaArgArgPheLeuOH (Kinetensin) mimetic pharmacophore
conserved Vitiligo post-trancripts domains

description: Vitiligo is a skin disorder characterized by selective melanocyte destruction and concomitant appearance of depigmented macules that over time enlarge, coalesce, and form patches. It has been suggested that vitiligo is, at least in part, caused by autoimmune responses mediated by cytotoxic T cells against melanocytes, causing depigmentation Immune responses contribute to the pathogenesis of vitiligo and target melanoma sometimes associated with vitiligo-like depigmentation in some melanoma patients. It has been perviously reported that the tyrosinase autoantigen was immunorecognized with the same molecular pattern by sera from vitiligo and melanoma patients. Five autoantigen peptides was found to compose the immunodominant antityrosinase response: aa95-104FMGFNCGNCK; aa175-182 LFVWMHYY; aa176-190FVWMHYYVSMDALLG; aa222-236IQKLTGDENFTIPYW, and aa233-247IPYWDWRDAEKCDIC. Synergistic therapies for the treatment of vitiligo are provided. The major therapies for the treatment of vitiligo a pigmentary disorder characterized by patchy depigmentation of skin are Psoralens plus UV-A, steroids, basic fibroblast growth factor (bFGF) peptide location or surgical procedures. Psoralens plus UV-A is effective in about 50% of cases, steroids are limitedly effective only in fast spreading cases of vitiligo and often reoccurs on stoppage of treatment. Surgical treatment is the last resort for vitiligo therapy, when all other therapies failed. It is limitedly effective. Basic fibroblast growth factor peptide(s) location was developed as a new mode therapy for the treatment of vitiligo. Therefore, SEQ ID NO: 01 VPHIPPN, SEQ ID NO: 02 MPPTQVS, SEQ ID NO: 03 QMHPWPP, SEQ ID NO: 1 1 LPLTPLP, SEQ ID NO: 12 QLNVNHQARADQ, SEQ ID NO: 13 TSASTRPELHYP, SEQ ID NO: 14 TFLPHQMHPWPP peptides, modified peptides and antibody or antibody fragments inhibiting the activity of MIA and can be used for treating vitiligo by inducing re-pigmentation. Fragment-based lead discovery is a method used for finding lead compounds as part of the drug discovery process. In this science project we perfomed an in silico ChemoProteomic Prediction-Scan for the generation of Antigenic PatternLFA-3/IgG fusion polypeptide aa95-104FMGFNCGNCK; aa175-182 LFVWMHYY; aa176-190FVWMHYYVSMDALLG; aa222-236IQKLTGDENFTIPYW, and aa233-247IPYWDWRDAEKCDICmimetic pharmacophore on conserved Vitiligo post-trancripts domains.
Magnetic Field and Atmospheric Chemical Abundances of the Magnetic Ap Star HD 318107
Creators: name: Bailey, J. D.
name: Landstreet, J. D.
name: Bagnulo, S.
name: Fossati, L.
name: Kochukhov, O.
name: Paladini, C.
name: Silvester, J.
name: Wade, G.
Publisher: name: arXiv
Year: 2011
Subjects: subject: Solar and Stellar Astrophysics (astro-ph.SR)
subject: FOS: Physical sciences

description: New spectra have been obtained with the ESPaDOnS spectropolarimeter supplemented with unpolarised spectra from the ESO UVES, UVES-FLAMES, and HARPS spectrographs of the very peculiar large-field magnetic Ap star HD 318107, a member of the open cluster NGC 6405. The available data provide sufficient material with which to re-analyse the first-order model of the magnetic field geometry and to derive abundances of Si, Ti, Fe, Nd, Pr, Mg, Cr, Mn, O, and Ca. The magnetic field structure was modelled with a low-order colinear multipole expansion, using coefficients derived from the observed variations of the field strength with rotation phase. The abundances of several elements were determined using spectral synthesis. After experiments with a very simple model of uniform abundance on each of three rings of equal width in co-latitude and symmetric about the assumed magnetic axis, we decided to model the spectra assuming uniform abundances of each element over the stellar surface. The new magnetic field measurements allow us to refine the rotation period of HD 318107 to P = 9.7088 +/- 0.0007 days. Appropriate magnetic field model parameters were found that very coarsely describe the (apparently rather complex) field moment variations. Spectrum synthesis leads to the derivation of mean abundances for the elements Mg, Si, Ca, Ti, Cr, Fe, Nd, and Pr. All of these elements except for Mg and Ca are strongly overabundant compared to the solar abundance ratios. There is considerable evidence of non-uniformity, for example in the different values of B_z found using lines of different elements. The present data set, while limited, is nevertheless sufficient to provide a useful first-order assessment of both the magnetic and surface abundance properties of HD 318107, making it one of the very few magnetic Ap stars of well-known age for which both of these properties have been studied.
description: 12 pages, 4 figures, accepted for publication in A&A