title: ÐÑÐµÐ½ÐºÐ° Ñ ÐµÐ»Ð°ÑÐ¸ÑÑÑÑÐ¸Ñ ÑÐ²Ð¾Ð¹ÑÑÐ² ÑÐµÐºÐ¾Ð¼Ð±Ð¸Ð½Ð°Ð½ÑÐ½Ð¾Ð³Ð¾ NdCTR1 Ð¿ÑÐ¸ ÑÐ¿Ð°ÑÐµÐ½Ð¸Ð¸ Ð½ÐµÐ¼Ð°ÑÐ¾Ð´ C. elegans, Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð½ÑÑ Ð¸Ð¾Ð½Ð°Ð¼Ð¸ ÑÐµÑÐµÐ±ÑÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Evaluation of the chelating properties of the recombinant NdCTR1 in the rescue of nematodes C. elegans treated with silver ions
Creators: name: Ð©ÑÐºÐ¸Ð½Ð°, ÐÐ½Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Ð¼ÐµÐ´Ñ
Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¼ Ð¼ÐµÐ´Ð¸
ATP7B
Ð±Ð¾Ð»ÐµÐ·Ð½Ñ ÐÐ¸Ð»ÑÑÐ¾Ð½Ð°
Ð½ÐµÐ¼Ð°ÑÐ¾Ð´Ð° C. elegans
CUA-1
ÑÐµÑÐµÐ±ÑÐ¾
copper
copper metabolism
Wilsons disease
nematode C. elegans
silver

description: ÐÐµÐ´Ñ â Ð¶Ð¸Ð·Ð½ÐµÐ½Ð½Ð¾ Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼ÑÐ¹ Ð¼Ð¸ÐºÑÐ¾ÑÐ»ÐµÐ¼ÐµÐ½Ñ Ð´Ð»Ñ Ð²ÑÐµÑ Ð¶Ð¸Ð²ÑÑ Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð¾Ð². ÐÐ°ÑÑÑÐµÐ½Ð¸Ñ Ð² ÑÐ°Ð±Ð¾ÑÐµ ÑÑÐ°Ð½ÑÐ¿Ð¾ÑÑÐ½Ð¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ñ Ð¼ÐµÐ´Ð¸ (Ð¢Ð¡Ð) Ð¿ÑÐ¸Ð²Ð¾Ð´ÑÑ Ðº ÑÐµÑÑÐµÐ·Ð½ÑÐ¼ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸ÑÐ¼. ÐÐ´Ð½Ð¸Ð¼ Ð¸Ð· Ð¿ÑÐ¸Ð¼ÐµÑÐ¾Ð² ÑÐ²Ð»ÑÐµÑÑÑ Ð±Ð¾Ð»ÐµÐ·Ð½Ñ ÐÐ¸Ð»ÑÑÐ¾Ð½Ð° (ÐÐ) â Ð°ÑÑÐ¾ÑÐ¾Ð¼Ð½Ð¾-ÑÐµÑÐµÑÑÐ¸Ð²Ð½Ð¾Ðµ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ðµ, Ð²ÑÐ·ÑÐ²Ð°ÐµÐ¼Ð¾Ðµ Ð¼ÑÑÐ°ÑÐ¸ÑÐ¼Ð¸ Ð² Ð³ÐµÐ½Ðµ ATP7B. ÐÐ»Ñ ÑÐµÑÐ°Ð¿Ð¸Ð¸ ÐÐ Ð¿ÑÐ¸Ð¼ÐµÐ½ÑÑÑÑÑ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÐµ ÑÐµÐ»Ð°ÑÐ¾ÑÑ, ÐºÐ¾ÑÐ¾ÑÑÐµ, Ð¾Ð´Ð½Ð°ÐºÐ¾, Ð¾Ð±Ð»Ð°Ð´Ð°ÑÑ ÑÑÐ´Ð¾Ð¼ Ð½ÐµÐ´Ð¾ÑÑÐ°ÑÐºÐ¾Ð² Ð¸ ÑÑÐ¶ÐµÐ»ÑÑ Ð¿Ð¾Ð±Ð¾ÑÐ½ÑÑ ÑÑÑÐµÐºÑÐ¾Ð². Ð Ð½Ð°ÑÑÐ¾ÑÑÐµÐ¹ ÑÐ°Ð±Ð¾ÑÐµ Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÐµÐ»Ð°ÑÐ¾ÑÐ° Ð¿ÑÐ¸ÑÐ¾Ð´Ð½Ð¾Ð³Ð¾ Ð¿ÑÐ¾Ð¸ÑÑÐ¾Ð¶Ð´ÐµÐ½Ð¸Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð»Ð¸ N-ÐºÐ¾Ð½ÑÐµÐ²Ð¾Ð¹ Ð´Ð¾Ð¼ÐµÐ½ Ð²ÑÑÐ¾ÐºÐ¾ Ð°ÑÑÐ¸Ð½Ð½Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ°-Ð¿ÐµÑÐµÐ½Ð¾ÑÑÐ¸ÐºÐ° Ð¼ÐµÐ´Ð¸ hCTR1 (NdCTR1), ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ ÐºÐ¾ÑÐ¾ÑÐ¾Ð³Ð¾ ÑÐµÐ»Ð°ÑÐ¸ÑÐ¾Ð²Ð°ÑÑ ÑÐµÑÐµÐ±ÑÐ¾ (Ag) Ð±ÑÐ»Ð° Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð° ÑÐ°Ð½ÐµÐµ Ð½Ð° Ð±Ð°ÐºÑÐµÑÐ¸ÑÑ E. coli ÑÑÐ°Ð¼Ð¼Ð° (DE3). Ag â Ð°Ð±Ð¸Ð¾Ð³ÐµÐ½Ð½ÑÐ¹ Ð¼ÐµÑÐ°Ð»Ð», Ð¿ÑÐ¸ ÑÑÐ¾Ð¼ Ð¸Ð¾Ð½Ñ Ag(I) Ð¸ Cu(I) Ð¸Ð·Ð¾ÑÐ»ÐµÐºÑÑÐ¾Ð½Ð½Ñ, ÑÑÐ¾ Ð´ÐµÐ»Ð°ÐµÑ Ag ÑÐ´Ð¾Ð±Ð½ÑÐ¼ Ð¸Ð½ÑÑÑÑÐ¼ÐµÐ½ÑÐ¾Ð¼ Ð´Ð»Ñ Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð¢Ð¡Ð. Ð ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð¼Ð¾Ð´ÐµÐ»ÑÐ½Ð¾Ð³Ð¾ Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð° Ð² Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ Ð½ÐµÐ¼Ð°ÑÐ¾Ð´Ñ Caenorhabditis elegans Ð´Ð¸ÐºÐ¾Ð³Ð¾ ÑÑÐ°Ð¼Ð¼Ð° N2 Ð¸ Ð¼ÑÑÐ°Ð½ÑÐ½Ð¾Ð³Ð¾ ÑÑÐ°Ð¼Ð¼Ð° H828Q, Ð¸Ð¼ÐµÑÑÐµÐ³Ð¾ ÑÐºÐ°Ð·Ð°Ð½Ð½ÑÑ Ð°Ð¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾ÑÐ½ÑÑ Ð·Ð°Ð¼ÐµÐ½Ñ Ð² Ð¼ÐµÐ´Ñ-ÑÑÐ°Ð½ÑÐ¿Ð¾ÑÑÐ¸ÑÑÑÑÐµÐ¹ ÐÐ¢Ð¤Ð°Ð·Ðµ CUA-1, Ð³Ð¾Ð¼Ð¾Ð»Ð¾Ð³Ð¸ÑÐ½Ð¾Ð¹ ATP7B, Ð¼ÐµÐ´Ñ-ÑÑÐ°Ð½ÑÐ¿Ð¾ÑÑÐ½Ð¾Ð¹ ÐÐ¢Ð¤Ð°Ð·Ðµ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°, Ð¸ Ð´ÐµÐ¼Ð¾Ð½ÑÑÑÐ¸ÑÑÑÑÐµÐ³Ð¾ ÑÐµÐ½Ð¾ÑÐ¸Ð¿ ÐÐ. Ð ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð½Ðµ Ð±ÑÐ»Ð¾ Ð²ÑÑÐ²Ð»ÐµÐ½Ð¾ ÑÐ¾ÐºÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð²Ð¾Ð·Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ NdCTR1 Ð½Ð° ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ðµ Ð½ÐµÐ¼Ð°ÑÐ¾Ð´ Ð´Ð¸ÐºÐ¾Ð³Ð¾ Ð¸ Ð¼ÑÑÐ°Ð½ÑÐ½Ð¾Ð³Ð¾ ÑÑÐ°Ð¼Ð¼Ð¾Ð². ÐÑÐ»Ð¸ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ñ Ð¿Ð¾Ð»ÑÐ»ÐµÑÐ°Ð»ÑÐ½ÑÐµ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ Ð½Ð¸ÑÑÐ°ÑÐ° ÑÐµÑÐµÐ±ÑÐ° Ð´Ð»Ñ Ð½ÐµÐ¼Ð°ÑÐ¾Ð´ Ð¾Ð±Ð¾Ð¸Ñ ÑÑÐ°Ð¼Ð¼Ð¾Ð². ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ Ð² ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐµ Ð¿Ð¾ Ð¾ÑÐµÐ½ÐºÐµ Ð²ÑÐ¶Ð¸Ð²Ð°ÐµÐ¼Ð¾ÑÑÐ¸ Ð½ÐµÐ¼Ð°ÑÐ¾Ð´, Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð½ÑÑ Ð¸Ð¾Ð½Ð°Ð¼Ð¸ ÑÐµÑÐµÐ±ÑÐ°, ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð¿Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÑÑ, ÑÑÐ¾ NdCTR1 Ð´Ð¾ÑÑÐ¾Ð²ÐµÑÐ½Ð¾ ÑÐ¿Ð°ÑÐ°ÐµÑ Ð½ÐµÐ¼Ð°ÑÐ¾Ð´ Ð¾Ð±Ð¾Ð¸Ñ ÑÑÐ°Ð¼Ð¼Ð¾Ð². Ð¢Ð°ÐºÐ¸Ð¼ Ð¾Ð±ÑÐ°Ð·Ð¾Ð¼, NdCTR1 ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ ÑÐµÐ»Ð°ÑÐ¸ÑÑÐµÑ ÑÐµÑÐµÐ±ÑÐ¾ Ð¸ Ð½ÑÐ¶Ð´Ð°ÐµÑÑÑ Ð² Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐ¸Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸ÑÑ ÐºÐ°Ðº Ð¿ÐµÑÑÐ¿ÐµÐºÑÐ¸Ð²Ð½ÑÐ¹ ÑÐµÐ»Ð°ÑÐ¾Ñ Ð´Ð»Ñ ÑÐµÑÐ°Ð¿Ð¸Ð¸ ÐÐ.
description: Copper is a vital trace element for all living organisms. Copper transport system (CTS) operation impairments can cause severe diseases. One example is Wilsons disease (WD) - autosomal recessive disorder caused by mutations in the ATP7B gene. Various chelators are used in WD treatment; however, they have several disadvantages and severe side effects. In this work, the N-terminal domain of human high-affinity copper uptake protein hCTR1 (NdCTR1), whose ability toÂ  chelate silver (Ag) was previously shown in experiments with E. coli (DE3) strain, was studied as a potential chelator of natural origin. Ag is an abiogenic metal, meanwhile the Ag(I) and Cu(I) ions are isoelectronic, which makes Ag a convenient tool for studying CTS. The nematodes Caenorhabditis elegans of the wild strain N2 and the mutant strain H828Q, which has the indicated amino acid substitution in the copper-transporting ATPase CUA-1 homologous to the human ATP7B ATPase and demonstrates the WD phenotype, were used as a model organism in this work. The study did not reveal any toxic effects of NdCTR1 on the development of wild and mutant strain nematodes. Semi-lethal concentrations of silver nitrate were determined for nematodes of both strains. The results obtained in the experiment on evaluating the survival of nematodes treated with silver ions have shown that NdCTR1 significantly rescues nematodes of both strains. Thus, the NdCTR1 effectively chelates silver and needs further research as a promising chelator for the treatment of WD.