Additional file 1 of Infusion of blood from mice displaying cerebral amyloidosis accelerates amyloid pathology in animal models of Alzheimer’s disease
Creators: name: Morales, Rodrigo
name: Duran-Aniotz, Claudia
name: Bravo-Alegria, Javiera
name: Lisbell D. Estrada
name: Shahnawaz, Mohammad
name: Hu, Ping-Ping
name: Kramm, Carlos
name: Morales-Scheihing, Diego
name: Urayama, Akihiko
name: Soto, Claudio
Publisher: name: figshare
Year: 2020
Subjects: subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Molecular Biology
subject: Neuroscience
subject: Immunology
subject: FOS: Clinical medicine
subject: FOS: Clinical medicine
subject: 111714 Mental Health
subject: FOS: Health sciences
subject: FOS: Health sciences
subject: Hematology

description: Additional file 1: Figure S1. Experimental strategy for animal treatments by intravenous infusions. Schematic summary of treatments in animals. A) applies to Experiments 1 and 2; B) applies to experiment 3; C) applies to Experiment 4; D) applies to Experiment 5, 6 and 7; and E) applies to experiment 8. Figure S2. APP/PS1 blood infusion increases cerebral Aβ deposition in younger animals carrying the same transgene (Experiment 4). A similar experiment to the one shown in Figure 1 was independently performed using APP/PS1 mice as explained in Materials and Methods. Image analysis was used to quantify the burden of 4G8-stained plaques in the cerebral cortex of mice receiving blood from 12 months old APP/PS1 (red squares) or age-matched WT littermates (black circles). The values were expressed as the mean ± SEM (n=4-5). Data was analyzed by Student’s t-test. * P&lt;0.05. Figure S3. Blood transfusion experiment using blood from 2-4 months old Tg2576 donors (Experiment 5). Young Tg2576 mice received blood from Tg2576 (red squares) or WT (black circles) mice ranging from 2 to 4.3 months old as explained in Materials and Methods. PBS-insoluble levels of Aβ42 measured by ELISA were assessed in the brain of blood-treated mice at the endpoint. The horizontal/dotted line was used to define the attack rate as explained in the body of the manuscript. Data was analyzed by Student’s t-test. n.s; no-significant. Figure S4. Blood transfusion experiment using blood from 7-9 months old Tg2576 donors (Experiment 6). Young Tg2576 mice received blood from Tg2576 (red squares) or WT (black circles) mice ranging from 7.9 to 9.2 months old as explained in Materials and Methods. PBS-insoluble levels of Aβ42 measured by ELISA were assessed in the brain of blood-treated mice at the endpoint. The horizontal/dotted line was used to define the attack rate as explained in the body of the manuscript. Data was analyzed by Student’s t-test. n.s; no-significant. Figure S5. Blood transfusion experiment using blood from 15-20 months old Tg2576 donors (Experiment 7). Young Tg2576 mice received blood from Tg2576 (red squares) or WT (black circles) mice ranging from 15.5 to 20 months old as explained in Materials and Methods. PBS-insoluble levels of Aβ42 measured by ELISA were assessed in the brain of experimental mice at the endpoint. The horizontal/dotted line was used to define the attack rate as explained in the body of the manuscript. Data was analyzed by Student’s t-test. n.s; no-significant. Figure S6. Intravenous administration of Tg2576 brain homogenate in young Tg2576 mice (Experiment 8). Young Tg2576 mice were intravenously injected with a 10% w/v Tg2576 brain homogenate (red squares) obtained from a 365 days old Tg2576 mice. Image analysis was used to quantify the burden of 4G8-stained plaques in the cerebral cortex and hippocampus. Mice receiving blood from age matched WT mice (black circles) were used as controls to define attack rates (same as depicted in Figure 1) as defined in the body of the manuscript. Table S1. Sex distribution of mice. The table below list the number of female and male mice used for each experiment included in this manuscript.