MOESM1 of Genome-wide analysis of polygalacturonase gene family from pear genome and identification of the member involved in pear softening
Creators: name: Zhang, Suling
name: Ma, Min
name: Zhang, Huping
name: Zhang, Shaoling
name: Qian, Ming
name: Zhang, Zhen
name: Luo, Weiqi
name: Fan, Jinbu
name: Liu, Zhiqiang
name: Wang, Libin
Publisher: name: figshare
Year: 2019
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Genetics
subject: Molecular Biology
subject: Evolutionary Biology
subject: Ecology
subject: Biological Sciences not elsewhere classified
subject: Plant Biology

description: Additional file 1: Figure S1. Multiple sequence alignment of PbrPGs. The red wireframe parts indicated four typical conserved domains of PbrPGs, which were named as domain I, II, III and IV, respectively. Figure S2. Comparative analysis of the 1.5 kb upstream of paralogous gene pairs. Divergence between upstream sequences of each paralogous gene pairs was measured by the GATA program (Nix and Eisen, 2005), with window size set as seven and lower cutoff score 12 bit. Solid dark lines connect similar regions and red broken lines connect matched regions in reversed orientation. Figure S3. Expression profiles of PbrPGs during development of different pear fruit. ‘Housui’ (a), ‘Kuerlexiangli’ (b), ‘Nanguo’ (c), ‘Starkrimson’ (d), ‘Yali’ (e) fruit were harvested from a commercial field at five developmental stages, including fruit-setting stage (period 1), physiological fruit dropping stage (period 2), fruit rapid enlargement stage (period 3), a month after fruit enlargement stage (period 4), and commercially mature stage (period 5). Data adapted from Zhang et al. (2015). Color scale at the top represented log2 transformed (FPKM + 1). Green indicated low level, black indicated a medium level, and red indicated high level. Figure S4. Dynamic changes of ethylene evolution and respiration rate during ‘Housui’ pear storage. Uniform and defect-free ‘Housui’ pear fruit were selected, randomly divided into several groups, packed with plastic bags, and then stored at 25 °C. Samples were taken every 6 d until decay rate over 20%. Data represented the mean value ± SE of three biological replicates. Different small letters with the same treatment mean significant difference among samples (p &lt; 0.05). Figure S5. qRT-PCR validation of the expression patterns of genes based on transcriptome analysis. Uniform and defect-free ‘Housui’ pear fruit were selected, randomly divided into several groups, packed with plastic bags, and then stored at 25 °C. Samples were taken every 6 d until decay rate over 20%. Data represented the mean ± SE of three biological replicates for qRT-PCR analysis. The expression level of PbrPG1 and Pbr5ERF39 at 0th d was set as 1.0. Different small letters with the same treatment mean significant difference among samples (p &lt; 0.05). Figure S6. Impact of 1-MCP and ethrel treatments on cortex firmness during pear storage. ‘Housui’ pears were harvested from an experimental orchard in Nanjing in 2017, and then divided into three treatments: (1) fumigated with 1.5 μL L− 1 1-MCP for 24 h, (2) dipped in 0.5 mL L− 1 ethrel for 5 min, and (3) dipped in 0.5 mL L− 1 H2O for 5 min (control). After treatments, fruits were packed with plastic bags and stored at 25 °C. Samples were taken every 6 d. Data represent the mean ± SE of three biological replicates. Different lowercase letters with the same treatment mean significant difference among samples, and different capital letters in the same sampling data mean significance among treatments (p &lt; 0.05). Figure S7. Impact of transient overexpression of PbrACO1 on PbrPG6 mRNAs and firmness of pear fruit. (a) Expression profile of PbrACO1 in samples. (b) Impact of transient on PbrPG6 mRNAs. (c) Impact of overexpression of PbrACO1 on cortex firmness. ‘Housui’ fruit infiltrated with the empty vector was used as control. Data represented the mean ± SE of three biological replicates. Different lowercase letters meant significance between samples (p &lt; 0.05). The expression level of PbrACO1/PbrPG6 in control fruit was set as 1.0. Vertical bars labeled with different small letters indicated significant difference between samples at p &lt; 0.05 level using Duncan’s multiple range test.