MOESM1 of Engineering protein production by rationally choosing a carbon and nitrogen source using E. coli BL21 acetate metabolism knockout strains
Creators: name: Terol, Gema Lozano
name: Gallego-Jara, Julia
name: Martínez, Rosa Sola
name: Díaz, Manuel Cánovas
name: Puente, Teresa Diego
Publisher: name: figshare
Year: 2019
Subjects: subject: Biophysics
subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Cell Biology
subject: Molecular Biology
subject: Physiology
subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: FOS: Chemical sciences
subject: 20199 Astronomical and Space Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: FOS: Physical sciences
subject: Developmental Biology
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: FOS: Health sciences
subject: 60506 Virology

description: Additional file 1: Table S1. List of plasmid used in this study. Table S2. List of primers employed in this study. Figure S1. E. coli BL21 wt grown at OD600 and acetate, glucose and glycerol extracellular concentrations. E. coli BL21 wt growth (black), extracellular acetate (blue) and glucose or glycerol consumption (green) in TB7 glucose (A), TB7 glycerol (B), MM9 glucose (C) and MM9 glycerol (D). Figure S2. E. coli BL21 ΔpatZ grown at OD600 and acetate, glucose and glycerol extracellular concentrations. E. coli BL21 ΔpatZ growth (black), extracellular acetate (blue) and glucose or glycerol consumption (green) in TB7 glucose (A), TB7 glycerol (B), MM9 glucose (C) and MM9 glycerol (D). Figure S3. E. coli BL21 ΔcobB grown at OD600 and acetate, glucose and glycerol extracellular concentrations. E. coli BL21 ΔcobB growth (black), extracellular acetate (blue) and glucose or glycerol consumption (green) in TB7 glucose (A), TB7 glycerol (B), MM9 glucose (C) and MM9 glycerol (D). Figure S4. E. coli BL21 Δacs grown at OD600 and acetate, glucose and glycerol extracellular concentrations. E. coli BL21 Δacs growth (black), extracellular acetate (blue) and glucose or glycerol consumption (green) in TB7 glucose (A), TB7 glycerol (B), MM9 glucose (C) and MM9 glycerol (D). Figure S5. E. coli BL21 ΔackA grown at OD600 and acetate, glucose and glycerol extracellular concentrations. E. coli BL21 ΔackA growth (black), extracellular acetate (blue) and glucose or glycerol consumption (green) in TB7 glucose (A), TB7 glycerol (B), MM9 glucose (C) and MM9 glycerol (D). Figure S6. E. coli BL21 Δpta grown at OD600 and acetate, glucose and glycerol extracellular concentrations. E. coli BL21 Δpta growth (black), extracellular acetate (blue) and glucose or glycerol consumption (green) in TB7 glucose (A), TB7 glycerol (B), MM9 glucose (C) and MM9 glycerol (D). Figure S7. Orotate extracellular concentration. Orotate extracellular concentration detected for E. coli BL21 wt, ΔpatZ, ΔcobB, Δacs, ΔackA and Δpta growing in TB7 glucose (A), TB7 glycerol (B), MM9 glucose (C) and MM9 glycerol (D). Figure S8. GFP fluorescence images. GFP fluorescence images of plating of E. coli BL21 wt (1) and mutant derivative (2: ΔpatZ; 3: ΔcobB; 4: Δacs; 5: ΔackA; 6: Δpta) cells expressing GFP. (A): plate image. (B): fluorescence plate image obtained with the Cy2 filter allowing GFP fluorescence detection with the Amersham Imager 600 (GE Healthcare). Figure S9. Relative fluorescence recorded during culture growth of E. coli BL21 wt in glycerol-TB7.