title: Ð¡Ð¾Ð·Ð´Ð°Ð½Ð¸Ðµ Ð¼Ð°ÐºÐµÑÐ° ÑÑÑÑÐ¾Ð¹ÑÑÐ²Ð° ÑÐµÐ³Ð¸ÑÑÑÐ°ÑÐ¸Ð¸ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸ Ð¾Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐ»ÑÐ¸Ð´Ð½ÑÑ ÑÐ¸Ð¿Ð¾Ð²
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Creation of a mock-up of a device for registering fluorescence from microfluidic chips
Creators: name: ÐÐ¾Ð±ÑÐµÑÐ¾Ð², Ð Ð¾Ð´Ð¸Ð¾Ð½
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð½Ð°Ñ ÑÐµÐ¿Ð½Ð°Ñ ÑÐµÐ°ÐºÑÐ¸Ñ (ÐÐ¦Ð )
Ð¼Ð¸ÐºÑÐ¾ÑÐ»ÑÐ¸Ð´Ð½ÑÐ¹ ÑÐ¸Ð¿
ÐÐÐ
ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ñ
ÐºÑÐ°ÑÐ¸ÑÐµÐ»Ð¸
ÑÐµÑÐ¼Ð¾ÑÐ¸ÐºÐ»ÐµÑ
Ð¾Ð¿ÑÐ¾Ð²Ð¾Ð»Ð¾ÐºÐ½Ð¾
Ð°Ð¼Ð¿Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ñ
polymerase chain reaction (PCR)
microfluidic chip
DNA
fluorescence
dyes
thermocycler
optical fiber
amplification

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description: The aim of this work is to create a working model for registering fluorescence from microfluidic chips during polymerase chain reaction (PCR). The paper discusses the main parameters of the PCR reaction in real time. Existing commer-cial instruments for real-time PCR reactions have been investigated and analyzed. The characteristics of the main elements used to create a prototype of a device for detecting fluorescence are given. The results of experiments in testing the performance of the elements of the model and microfluidic chips are presented. The ability of a photodetector (digital camera) to register the fluorescence signal of a fluorescein solution with concentrations of 10â¾âµ, 10â¾â¶ and 10â¾â·, which fills a microfluidic chip, has been tested. The efficiency of the assembled model has been demonstrated when carrying out a PCR reaction in real time. The model assem-bled as a result of the work can be further used for setting real-time PCR on microfluidic chips in biological and medical research. The subject of research in this work is a mock-up of a device for detecting fuorescence from microfluidic chips; research methods are: fluorometry, spectro-photometry and PCR analysis on a mycrofluidic chip.