Cordyceps yaoluopingensis Y. Yang, Y. P. Xiao &amp; X. Luo 2022, sp. nov.
Creators: name: Yang, Yu
name: Xiao, Yuan-Ping
name: Cheng, Yan-Ni
name: Xu, Fei-Yan
name: Han, Hao-Ming
name: Luo, Xia
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Fungi
Ascomycota
Sordariomycetes
Hypocreales
Cordycipitaceae
Cordyceps
Cordyceps yaoluopingensis

description: <i>Cordyceps yaoluopingensis</i> Y.Yang, Y.P. Xiao &amp; X. Luo, <i>sp. nov.</i> <i>Index Fungorum number</i>: IF559645, Facesoffungi number: FoF 10839 <i>Etymology</i>: the new species is named after China, Anhui, Yaoluoping Nature Reserve, its type locality <i>Holotype</i>: CZ01 (ex-type living cultures: CGMCC 23076) <i>Host</i>: Pupae of Lepidoptera with white surface mycelium, collected from the underside of leaf litter and emanating from multiple parts of the host. <i>Sexual morph</i>: Undetermined. <i>Asexual morph</i>: <i>Isaria</i> -like. <i>Synnemata</i> arising from the pupae of Lepidoptera, erect, multiple, flexuous, white, fleshy, up to 0.5-0.8 cm long, with terminal branches of 1–2 × 1.2–1.6 mm. <i>Conidiophores</i> 20–50 × 2.2–4.1 μm (<i>x</i> = 35 × 4.25 μm, n = 30), complex verticillate branches with whorls of 2 to 7 phialides, hyaline, smooth-walled. <i>Phialides</i> 6.9–9.8 × 2–3.5 µm (<i>x</i> = 8.35 × 2.75 µm, n = 50), verticillate, globose basal portion with a thin neck about 0.5 µm wide. <i>Conidia</i> 2.0–5.0 × 1.5–2.6 µm (<i>x</i> = 3.5 × 2.05 µm, n = 50), one-celled, broadly fusiform, hyaline, smooth-walled. <i>Culture characteristics</i>: Colonies on PDA medium, moderately fast-growing, 2–3 cm diameter in 14 days at 25 °C, white to yellowish, cottony, with low mycelial density, circular. <i>Synnemata</i> 0.6–1.6 × 0.1–0.4 cm (<i>x</i> = 1.1 × 0.25 cm, n = 20), emerging after 15 days in the margin of the colony, single or branched as multiple branches. <i>Hyphae</i> 2.1–4.1 µm wide, smooth-walled, branched, septate, hyaline. <i>Conidiophores</i> 34–62 × 2.5–3.4 µm (<i>x</i> = 48 × 2.95 µm, n = 30), erect, arising from the aerial and prostrate hyphae, verticillate, with phialides in whorls of 3 to 8, smooth-walled, cylindrical to ellipsoidal. <i>Phialides</i> 7.1–11.2 × 3.1–4.2 µm (<i>x</i> = 6.1 × 3.65 µm, n = 50), verticillate, consisting of a swollen, more or less ellipsoidal basal portion, tapering into a thin neck, 0.5–0.8 µm wide. <i>Conidia</i> 4.3–7.0 × 2.1–3.7 µm (<i>x</i> = 5.65 × 2.9 µm, n = 50), hyaline, one-celled, ovoid or fusiform. <i>Material examined</i>: CHINA, Anhui Province, Yaoluoping Nature Reserve, at 1187m, 116.05 E, 30.98 N, 1 October 2018, collected by Luo X (Holotype: CZ01), ex-type living culture: CGMCC 23076. Notes: In the phylogenetic tree, <i>Cordyceps yaoluopingensis</i> shared the same branches with <i>C.tenuipes</i> and <i>C. takaomontana</i> (Figure 2). Six loci of <i>C. tenuipes</i> (YFCC 4266), <i>C. tenuipes</i> (BUC 394), <i>C. tenuipes</i> (ARSEF 5135) and <i>C. takaomontana</i> (ANDES-F 1274) shared between 96% (<i>tef1-α</i>), 98% and 99% (<i>rpb1</i>) and 91% (<i>rpb2</i>) similarity with <i>C. yaoluopingensis</i>. However, they also can be distinguished by morphological characters. <i>C. yaoluopingensis</i> has shorter synnemata, longer phialides, and bigger ovoid or fusiform conidia compared to <i>C. takaomontana</i> (Castillo <i>et al.</i> 2018, Table 2). <i>Cordyceps tenuipes</i> from Japan were initially described and illustrated by Samson (Samson 1974). It differs from <i>C. yaoluopingensis</i> in having longer synnemata and shorter phialides (Samson 1974, TABLE 2). Liang <i>et al.</i> (2003) was initially described a specimen of <i>C. takaomontana</i> (LFRGU86-913) from China, growing on Lepidoptera larva. Morphologically, LFRGU86-913 differs from the new species due to its longer phialides and conidia (Liang <i>et al.</i> 2003). Meanwhile, Li <i>et al.</i> (2007) recorded another description of <i>C. takaomontana</i> (TTZ060731-62) from China. The specimen (TTZ060731-62) developed on a large and nearly rounded (slightly ovate) cocooned pupae (Lepidoptera), with its asexual morph matching Samson’s description (Li <i>et al.</i> 2007). However, both Chinese specimens are missing DNA sequence data. Chen <i>et al.</i> (2019b) provided sequence data for <i>C. tenuipes</i> strains YFCC 4266, BUC 394, and ARSEF 5135, but not morphological data. To summarise, sufficient evidence from morphological and molecular data suggests that <i>C. yaoluopingensis</i> is a novel <i>Cordyceps</i> species.
description: Published as part of <i>Yang, Yu, Xiao, Yuan-Ping, Cheng, Yan-Ni, Xu, Fei-Yan, Han, Hao-Ming &amp; Luo, Xia, 2022, Cordyceps yaoluopingensis sp. nov., a new entomopathogenic species from China, pp. 278-290 in Phytotaxa 571 (3)</i> on pages 282-284, DOI: 10.11646/phytotaxa.571.3.2, http://zenodo.org/record/7284647
description: {"references": ["Castillo, L. P., Osorio, A. Vargas, N., Sanjuan, T., Grajales, A. &amp; Restrepo, S. (2018) Genetic diversity of the entomopathogenic fungus Cordyceps tenuipes in forests and butterfly gardens in Quindio, Colombia. Fungal Biology 122 (9): 891 - 899. https: // doi. org / 10.1016 / j. funbio. 2018.05.003", "Samson, R. (1974) Paecilomyces and some allied Hyphomycetes. Studies in Mycology 6.", "Liang, Z. Q., Liu, A. Y., Liu, M. H. &amp; Kang, J. C. (2003) The genus Cordyceps and its allies from the Kuankuoshui Reserve in Guizhou III. Fungal Diversity 14: 95 - 101.", "Li, C. R., Zuo, D. P., Nam, S. H., Pu, S. C., Fan, M. Z. &amp; Li, Z. Z. (2007) Cordyceps takaomontana and its anamorph Paecilomyces tenuipes. Mycosystema 26 (2): 217 - 220. https: // doi. org / 10.13346 / j. mycosystema. 2007.02.012", "Chen, Z., Wang, Y., Dai, Y., Chen, K., Xu, L. &amp; He, Q. (2019 b) Species diversity and seasonal fluctuation of entomogenous fungi of Ascomycota in Taibaoshan Forest Park in western Yunnan. Biodiversity Science 27 (9): 993 - 1001."]}