cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
title: High precision quark mass determinations and studies of meson properties using lattice QCD
Creators: name: Hatton, Daniel
Publisher: name: University of Glasgow
Year: 2020
Subjects: subject: lattice QCD, quark masses.

description: Strongly interacting systems such as the internal dynamics of the myriad bound states seen in particle physics experiments are notoriously difficult to obtain a precise quantitative theoretical description of. Without systematically improvable methods of analysis of such system a vast region of particle physics phenomenology is left without proper confrontation with theory and systematic uncertainties arising from knowledge of fundamental particle physics parameters in the strong sector are difficult to control. Lattice quantum chromodynamics (QCD) has arisen as a methodological solution to the calculational difficulties raised by the theory of the strong interaction. The main testing ground for high precision calculations utilised here is the properties of the two lowest lying states of the chamronium spectrum, the $\eta_c$ and the $J/\psi$. Certain properties of these mesons are experimentally determined to high precision which makes them good targets for high precision lattice QCD calculations. At this level of precision it becomes necessary to include at least leading order electromagnetic corrections. This is addressed in this thesis with the inclusion of quenched QED in the study of charmonium properties. In various calculations renormalisation factors need to be computed to high precision. The technique of nonperturbatively computing renormalisation factors in a momentum subtraction scheme on the lattice provides a computationally cheap method for the determination of these factors and is examined using the Highly Improved Staggered Quark (HISQ) action. The charmonium calculations are then extended to higher masses leading up to the $b$ quark mass allowing for comparisons with experimental results. It is demonstrated that this method offers a way to improve on the precision of previous lattice calculations that used nonrelativistic quark actions. Finally, sub-percent determinations of the strange and charm quark masses are presented, making use of nonperturbatively determined mass renormalisation factors. These are shown to agree, at this high level of precision, with other lattice calculations that use different methodologies, indicating that the systematic uncertainties are appropriately controlled.
Molecular Basis for Atovaquone Binding to the Cytochrome bc 1 Complex
Creators: name: Hunte, Carola
name: Meunier, Brigitte
name: Meshnick, Steve
name: Lange, Benjamin B.
name: Kessl, Jacques J.
name: Zwicker, Klaus
name: Hill, Philip
name: Merbitz-Zahradnik, Torsten
name: Pálsdóttir, Hildur
name: Trumpower, Bernard L.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2003

description: Atovaquone is a substituted 2-hydroxynaphthoquinone that is used therapeutically to treat Plasmodium falciparum malaria, Pneumocystis carinii pneumonia, and Toxoplasma gondii toxoplasmosis. It is thought to act on these organisms by inhibiting the cytochrome bc1 complex. We have examined the interaction of atovaquone with the bc1 complex isolated from Saccharomyces cerevisiae, a surrogate, nonpathogenic fungus. Atovaquone inhibits the bc1 complex competitively with apparent Ki = 9 nm, raises the midpoint potential of the Rieske iron-sulfur protein from 285 to 385 mV, and shifts the g values in the EPR spectrum of the Rieske center. These results indicate that atovaquone binds to the ubiquinol oxidation pocket of the bc1 complex, where it interacts with the Rieske iron-sulfur protein. A computed energy-minimized structure for atovaquone liganded to the yeast bc1 complex suggests that a phenylalanine at position 275 of cytochrome b in the bovine bc1 complex, as opposed to leucine at the equivalent position in the yeast enzyme, is responsible for the decreased sensitivity of the bovine bc1 complex (Ki = 80 nm) to atovaquone. When a L275F mutation was introduced into the yeast cytochrome b, the sensitivity of the yeast enzyme to atovaquone decreased (Ki = 100 nm) with no loss in activity, confirming that the L275F exchange contributes to the differential sensitivity of these two species to atovaquone. These results provide the first molecular description of how atovaquone binds to the bc1 complex and explain the differential inhibition of the fungal versus mammalian enzymes.
Supplementary Figure 1 from α-Amylase immobilization on amidoximated acrylic microfibres activated by cyanuric chloride
Creators: name: Yaaser Q. Almulaiky
name: Aqlan, Faisal M.
name: Musab Aldhahri
name: Baeshen, Mohammed
name: Khan, Tariq Jamal
name: Khan, Khalid A.
name: Afifi, Mohamed
name: AL-Farga, Ammar
name: Mohiuddin Khan Warsi
name: Alkaled, Mohammed
name: Alayafi, Aisha A. M.
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: Biochemistry
subject: 90301 Biomaterials
subject: FOS: Medical engineering

description: Effect of immobilization time
Current Status of OpenFF and our Near-Term Roadmap
Creators: name: Mobley, David L.
Publisher: name: Zenodo
Year: 2020
Subjects: Open Force Field Initiative
force fields
benchmarking
data selection
training

description: This presentation is a part of the Open Force Field Virtual Meeting 2020. Abstract: This presentation provides an overview of the recent milestones achieved by the Open Force Field Initiative and the near-term plans in force field development and benchmarking.
Molecular Basis of Calmodulin Binding to Cardiac Muscle Ca 2 + Release Channel (Ryanodine Receptor)
Creators: name: Evans, Kelly E.
name: Xu, Le
name: Yamaguchi, Naohiro
name: Pasek, Daniel A.
name: Meissner, Gerhard
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2003

description: Calmodulin (CaM) is a ubiquitous Ca2+-binding protein that regulates the ryanodine receptors (RyRs) by direct binding. CaM inhibits the skeletal muscle ryanodine receptor (RyR1) and cardiac muscle receptor (RyR2) at >1 microm Ca2+ but activates RyR1 and inhibits RyR2 at <1 microm Ca2+. Here we tested whether CaM regulates RyR2 by binding to a highly conserved site identified previously in RyR1. Deletion of RyR2 amino acid residues 3583-3603 resulted in background [35S]CaM binding levels. In single channel measurements, deletion of the putative CaM binding site eliminated CaM inhibition of RyR2 at Ca2+ concentrations below and above 1 microm. Five RyR2 single or double mutants in the CaM binding region (W3587A, L3591D, F3603A, W3587A/L3591D, L3591D/F3603A) eliminated or greatly reduced [35S]CaM binding and inhibition of single channel activities by CaM depending on the Ca2+ concentration. An RyR2 mutant, which assessed the effects of 4 amino acid residues that differ between RyR1 and RyR2 in or flanking the CaM binding domain, bound [35S]CaM and was inhibited by CaM, essentially identical to wild type (WT)-RyR2. Three RyR1 mutants (W3620A, L3624D, F3636A) showed responses to CaM that differed from corresponding mutations in RyR2. The results indicate that CaM regulates RyR1 and RyR2 by binding to a single, highly conserved CaM binding site and that other RyR type-specific sites are likely responsible for the differential functional regulation of RyR1 and RyR2 by CaM.
Multifunctional Roles for the PH Domain of Dbs in Regulating Rho GTPase Activation
Creators: name: Rossman, Kent L.
name: Snyder, Jason T.
name: Sondek, John
name: Rojas, Rafael J.
name: Cheng, Li
name: Whitehead, Ian P.
name: Mahon, Gwendolyn M.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2003

description: Dbl family members are guanine nucleotide exchange factors specific for Rho guanosine triphosphatases (GTPases) and invariably possess tandem Dbl (DH) and pleckstrin homology (PH) domains. Dbs, a Dbl family member specific for Cdc42 and RhoA, exhibits transforming activity when overexpressed in NIH 3T3 mouse fibroblasts. In this study, the PH domain of Dbs was mutated to impair selectively either guanine nucleotide exchange or phosphoinositide binding in vitro and resulting physiological alterations were assessed. As anticipated, substitution of residues within the PH domain of Dbs integral to the interface with GTPases reduced nucleotide exchange and eliminated the ability of Dbs to transform NIH 3T3 cells. More interestingly, substitutions within the PH domain that prevent interaction with phosphoinositides yet do not alter in vitro activation of GTPases also do not transform NIH 3T3 cell and fail to activate RhoA in vivo despite proper subcellular localization. Therefore, the PH domain of Dbs serves multiple roles in the activation of GTPases and cannot be viewed as a simple membrane-anchoring device. In particular, the data suggest that binding of phosphoinositides to the PH domain within the context of membrane surfaces may direct orientations or conformations of the linked DH and PH domains to regulate GTPases activation.
Nitric Oxide, NOC-12, and S -Nitrosoglutathione Modulate the Skeletal Muscle Calcium Release Channel/Ryanodine Receptor by Different Mechanisms: AN ALLOSTERIC FUNCTION FOR O2INS-NITROSYLATION OF THE CHANNEL
Creators: name: Sun, Junhui
name: Eu, Jerry P.
name: Meissner, Gerhard
name: Stamler, Jonathan S.
name: Xu, Le
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2003

description: The skeletal muscle Ca(2+) release channel/ryanodine receptor (RyR1) contains approximately 50 thiols per subunit. These thiols have been grouped according to their reactivity/responsiveness toward NO, O(2), and glutathione, but the molecular mechanism enabling redox active molecules to modulate channel activity is poorly understood. In the case of NO, very low concentrations (submicromolar) activate RyR1 by S-nitrosylation of a single cysteine residue (Cys-3635), which resides within a calmodulin binding domain. S-Nitrosylation of Cys-3635 only takes place at physiological tissue O(2) tension (pO(2); i.e. approximately 10 mm Hg) but not at pO(2) approximately 150 mm Hg. Two explanations have been offered for the loss of RyR1 responsiveness to NO at ambient pO(2), i.e. Cys-3635 is oxidized by O(2) versus O(2) subserves an allosteric function (Eu, J. P., Sun, J. H., Xu, L., Stamler, J. S., and Meissner, G. (2000) Cell 102, 499-509). Here we report that the NO donors NOC-12 and S-nitrosoglutathione both activate RyR1 by release of NO but do so independently of pO(2). Moreover, NOC-12 activates the channel by S-nitrosylation of Cys-3635 and thereby reverses channel inhibition by calmodulin. In contrast, S-nitrosoglutathione activates RyR1 by oxidation and S-nitrosylation of thiols other than Cys-3635 (and calmodulin is not involved). Our results suggest that the effect of pO(2) on RyR1 S-nitrosylation is exerted through an allosteric mechanism.
The influence of physical effort in reminder-setting on strategic offloading behaviour
Creators: name: Gilbert, Sam
name: Chiu, Gavin
Publisher: name: OSF Registries
Year: 2023
Subjects: subject: Cognitive Psychology
subject: Social and Behavioral Sciences
subject: Psychology
subject: FOS: Psychology

description: Intention offloading involves the use of external reminders such as diaries, to-do lists, and digital alerts to help us remember delayed intentions. Recent studies have provided evidence for various cognitive and metacognitive factors that guide intention offloading, but little research has investigated the physical cost of reminder-setting itself. Here we present two pre-registered experiments investigating how the cost of physical reminder effort influences strategic intention offloading under different levels of memory load. At all memory loads, reminder-setting was reduced when it was more effortful. The ability to set reminders allowed participants to compensate for the influence of memory load on accuracy in the low-effort condition; this effect was attenuated in the high-effort condition. Contrary to prediction, effort had the greatest effect on reminder-setting at intermediate memory loads. We speculate that the physical costs of reminder-setting might have the greatest impact when participants are uncertain about the correct strategy. These results demonstrate the importance of physical reminder effort as one of the factors relevant to cost-benefit decision-making about cognitive offloading strategies.
Supplementary Figure 5 from α-Amylase immobilization on amidoximated acrylic microfibres activated by cyanuric chloride.
Creators: name: Yaaser Q. Almulaiky
name: Aqlan, Faisal M.
name: Musab Aldhahri
name: Baeshen, Mohammed
name: Khan, Tariq Jamal
name: Khan, Khalid A.
name: Afifi, Mohamed
name: AL-Farga, Ammar
name: Mohiuddin Khan Warsi
name: Alkaled, Mohammed
name: Alayafi, Aisha A. M.
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: Biochemistry
subject: 90301 Biomaterials
subject: FOS: Medical engineering

description: Effect of pH, temperature, km
(1717) Louisa Jane Haigh
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Supplementary Figure 2 from α-Amylase immobilization on amidoximated acrylic microfibres activated by cyanuric chloride
Creators: name: Yaaser Q. Almulaiky
name: Aqlan, Faisal M.
name: Musab Aldhahri
name: Baeshen, Mohammed
name: Khan, Tariq Jamal
name: Khan, Khalid A.
name: Afifi, Mohamed
name: AL-Farga, Ammar
name: Mohiuddin Khan Warsi
name: Alkaled, Mohammed
name: Alayafi, Aisha A. M.
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: Biochemistry
subject: 90301 Biomaterials
subject: FOS: Medical engineering

description: FTIR
Tunable deep-subwavelength superscattering using graphene monolayers
Creators: name: Li, R. J.
name: Lin, X.
name: Lin, S. S.
name: Liu, X.
name: Chen, H. S.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Optics (physics.optics)
subject: Materials Science (cond-mat.mtrl-sci)
subject: FOS: Physical sciences

description: In this Letter, we theoretically propose for the first time that graphene monolayers can be used for superscatterer designs. We show that the scattering cross section of the bare deep-subwavelength dielectric cylinder is markedly enhanced by six orders of magnitude due to the excitation of the first-order resonance of graphene plamons. By utilizing the tunability of the plasmonic resonance through tuning graphene's chemical potential, the graphene superscatterer works in a wide range of frequencies from several terahertz to tens of terahertz.
description: This paper has been accepted by Optics Letter
Présentation du principe et du logiciel de fusion de données appliqués aux ENDs en Génie Civil
Creators: name: Garnier, Vincent
name: Loudiyi, Mohammed
name: Rabone, Jeremy
Publisher: name: Academic Journal of Civil Engineering
Year: 2021

description: La surveillance des ouvrages en béton par des Essais Destructifs (ED) sur prélèvements peut être remplacée avec un moindre coût et sans endommagement de la structure par des Essais Non Destructifs (END). Les techniques non destructives peuvent être employées dans les ouvrages en béton à des fins de détection ou de quantification. Dans le premier cas, il s’agit de détecter la présence d’un défaut (fissure par exemple) en combinant plusieurs END et en s’appuyant sur leurs sensibilités aux changements. Dans le deuxième cas, il s’agit de quantifier une grandeur à partir des END en s’appuyant sur des modèles de conversion. Ces démarches de combinaison, de conversion et d’optimisation ont été implantées dans deux outils de fusion MATLAB. Nous consacrons ce document pour présenter l’outil de quantification des caractéristiques d’un béton. Une brève description du principe de la méthode de fusion, de l’outil développé ainsi qu’une application concrète, sur des données issues de projets nationaux
description: Academic Journal of Civil Engineering, Vol 38 No 2 (2021): Special Issue - Diagnobéton 2021
An introduction to some imperfections of CCD sensors
Creators: name: Astier, Pierre
Publisher: name: arXiv
Year: 2015
Subjects: subject: Instrumentation and Methods for Astrophysics (astro-ph.IM)
subject: FOS: Physical sciences

description: CCD sensors do not deliver a perfect image of the light they receive. Beyond the well known linear image smearing due to diffusion of charges during their drift towards the pixel wells, non-linear effects are at play in these sensors. We now have ample evidence for both a flux- dependent and static image distortions, especially but not only, on deep-depleted CCDs. For large surveys relying on CCD sensors, these effects should now be taken into account when reducing data. We present here a summary of current results on sensor characterization and mitigation methods.
description: 10 pages. Contribution to the proceedings of PACCD14. Accepted for publication in JINST
Supplementary Table 1 from α-Amylase immobilization on amidoximated acrylic microfibres activated by cyanuric chloride
Creators: name: Yaaser Q. Almulaiky
name: Aqlan, Faisal M.
name: Musab Aldhahri
name: Baeshen, Mohammed
name: Khan, Tariq Jamal
name: Khan, Khalid A.
name: Afifi, Mohamed
name: AL-Farga, Ammar
name: Mohiuddin Khan Warsi
name: Alkaled, Mohammed
name: Alayafi, Aisha A. M.
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: Biochemistry
subject: 90301 Biomaterials
subject: FOS: Medical engineering

description: Effect of cyanuric chloride percentage and pH on the immobilization efficiency
Diagnostic de la corrosion des structures en béton armé par mesure simultanée du potentiel de corrosion, de la résistivité du béton d’enrobage et de la vitesse de corrosion des armatures
Creators: name: Deby, Fabrice
Publisher: name: Academic Journal of Civil Engineering
Year: 2021

description: Ce travail fait partie du projet DIAMOND (https://www.projet-diamond.com/) qui a permis de développer une solution technique complète et innovante de diagnostic de la corrosion. Un nouveau type de sonde a été mis au point permettant à la fois de mesurer le potentiel de corrosion, d’évaluer la résistivité du béton d’enrobage et la vitesse de corrosion des armatures. Le design géométrique de la sonde est simple : un courant est injecté par une contre-électrode annulaire et la réponse en potentiel est mesurée au centre, au niveau d’une électrode de référence. Afin d’inverser les mesures réalisées en surface pour en déduire la résistivité du béton d’enrobage et la vitesse de corrosion des armatures, l’ensemble du dispositif expérimental a fait l’objet d’un jumeau numérique fondé sur un modèle 3D éléments finis.
description: Academic Journal of Civil Engineering, Vol 38 No 2 (2021): Special Issue - Diagnobéton 2021
Overexpression of the Linker Histone-binding Protein tNASP Affects Progression through the Cell Cycle
Creators: name: Alekseev, Oleg M.
name: Bencic, David C.
name: Richardson, Richard T.
name: Widgren, Esther E.
name: O'Rand, Michael G.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2003

description: NASP is an H1 histone-binding protein that is cell cycle-regulated and occurs in two major forms: tNASP, found in gametes, embryonic cells, and transformed cells; and sNASP, found in all rapidly dividing somatic cells (Richardson, R. T., Batova, I. N., Widgren, E. E., Zheng, L. X., Whitfield, M., Marzluff, W. F., and O'Rand, M. G. (2000) J. Biol. Chem. 275, 30378-30386). When full-length tNASP fused to green fluorescent protein (GFP) is transiently transfected into HeLa cells, it is efficiently transported into the nucleus within 2 h after translation in the cytoplasm, whereas the NASP nuclear localization signal (NLS) deletion mutant (NASP-DeltaNLS-GFP) is retained in the cytoplasm. In HeLa cells synchronized by a double thymidine block and transiently transfected to overexpress full-length tNASP or NASP-DeltaNLS, progression through the G(1)/S border is delayed. Cells transiently transfected to overexpress the histone-binding site (HBS) deletion mutant (NASP-DeltaHBS) or sNASP were not delayed in progression through the G(1)/S border. By using a DNA supercoiling assay, in vitro binding data demonstrate that H1 histone-tNASP complexes can transfer H1 histones to DNA, whereas NASP-DeltaHBS cannot. Measurement of NASP mobility in the nucleus by fluorescence recovery after photobleaching indicates that NASP mobility is virtually identical to that reported for H1 histones. These data suggest that NASP-H1 complexes exist in the nucleus and that tNASP can influence cell cycle progression through the G(1)/S border through mediation of DNA-H1 histone binding.
A field-portable colorimetric method for the measurement of peracetic acid vapors: a comparison of glass and plastic impingers
Creators: name: Stastny, Angela L.
name: Doepke, Amos
name: Streicher, Robert P.
Publisher: name: Taylor & Francis
Year: 2022
Subjects: subject: Biophysics
subject: Biochemistry
subject: Space Science
subject: 29999 Physical Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Genetics
subject: Molecular Biology
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences

description: A method for measuring peracetic acid vapors in air using impinger sampling and field-portable colorimetric analysis is presented. The capture efficiency of aqueous media in glass and plastic impingers was evaluated when used for peracetic acid vapor sampling. Measurement of peracetic acid was done using an N,N-diethyl-p-phenylenediamine colorimetric method with a field-portable spectrometer. The linearity of the N,N-diethyl-p-phenylenediamine method was determined for peracetic acid both in solution and captured from vapor phase using glass or plastic impingers. The Limits of Detection for the glass and plastic impingers were 0.24 mg/m3 and 0.28 mg/m3, respectively, for a 15 L air sample. The Limits of Quantitation were 0.79 mg/m3 and 0.92 mg/m3 for the glass and plastic impingers, respectively. Both metrics were below the American Conference of Governmental Industrial Hygienists Threshold Limit Value Short-Term Exposure Limit of 1.24 mg/m3 (0.4 ppmv) during a 15-min period. The impinger sampling method presented herein allows for an easy-to-use and rapid in-field measurement that can be used for evaluating occupational exposure to peracetic acid.
Phonons, pions and quasi-long-range order in spatially modulated chiral condensates
Creators: name: Hidaka, Yoshimasa
name: Kamikado, Kazuhiko
name: Kanazawa, Takuya
name: Noumi, Toshifumi
Publisher: name: arXiv
Year: 2015
Subjects: subject: High Energy Physics - Phenomenology (hep-ph)
subject: High Energy Physics - Theory (hep-th)
subject: Nuclear Theory (nucl-th)
subject: FOS: Physical sciences

description: We investigate low-energy fluctuations in the real kink crystal phase of dense quark matter within the Nambu--Jona-Lasinio model. The modulated chiral condensate breaks both the translational symmetry and chiral symmetry spontaneously, which leads to the appearance of phonons and pions that are dominant degrees of freedom in the infrared. Using the Ginzburg-Landau expansion near the Lifshitz point, we derive elastic free energies for phonons and pions in dependence on the temperature and chemical potential. We show that the one-dimensional modulation is destroyed by thermal fluctuations of phonons at nonzero temperature, and compute the exponent that characterizes the anisotropic algebraic decay of quasicondensate correlations at long distance. We also estimate finite-volume effects on the stability of the real kink crystal and briefly discuss the possibility of its existence in neutron stars.
description: 17 pages, 9 figures. v2: minor changes, published version
Detection and evaluation of macro-cracks and micro-cracking by using automated surface wave non-contact scanners
Creators: name: Piwakowski, Bogdan
name: Ciccarone, Charles
name: Skoczylas, Frederic
name: Drelich, Radoslaw
name: Henaut, Jean-Marie
Publisher: name: Academic Journal of Civil Engineering
Year: 2021

description: The goal of the presented work is the detection and evaluation of cracks, in the VeRCoRs mock-up which constitutes the double-wall containment building of a Nuclear Power Plant in scale 1/3. The experiments are conducted in situ, on the inner wall of the VeRCoRs mock-up. The detection is performed by means of two automated scanners designed for the non-destructive non-contact control of concrete using surface waves (SW). The SW signal assures the penetration in a concrete wall between 1 to 60 cm. The scanners provide the non-contact measure which permits to avoid a modification of the concrete surface by a coupling agent and a time consuming coupling procedure. The results, obtained during pressure test in the VeRCoRs mock-up, prove that the scanner can be successfully used in the detection of micro-cracking, of cracks location, the evaluation of their opening and for in the progressive aging of the mock-up.
description: Academic Journal of Civil Engineering, Vol 38 No 2 (2021): Special Issue - Diagnobéton 2021
(1709) Hannah Maria Willans
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
PB1 Domains of MEKK2 and MEKK3 Interact with the MEK5 PB1 Domain for Activation of the ERK5 Pathway
Creators: name: Nakamura, Kazuhiro
name: Johnson, Gary L.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2003

description: MEKK2 and MEKK3 are MAPK kinase kinases that activate the ERK5 pathway by phosphorylating and activating the MAPK kinase, MEK5. Activated MEK5 then phosphorylates and activates ERK5. PB1 domains were first defined in the p67phox and Bem1p proteins and have been shown to mediate protein-protein heterodimerization. A PB1 domain is encoded within the N-terminal portion of MEKK2, MEKK3, and MEK5. Herein, we analyze the functional role of MEKK2, MEKK3, and MEK5 PB1 domains in the ERK5 activation pathway. The PB1 domains of MEKK2 and MEKK3 bind the PB1 domain of MEK5 but do not significantly homo- or heterodimerize with one another in vitro. Furthermore, co-immunoprecipitation of MEKK2 and MEK5 from cell lysates shows that they form a complex in vivo. Deletion or mutation of the MEKK2 PB1 domain abolishes MEKK2-MEK5 complexes, demonstrating that the PB1 domain interaction is required for MEKK2-MEK5 interactions. Expression in cells of the MEKK2 or MEKK3 PB1 domain inhibits ERK5 activation, whereas expression of a mutant MEKK2 unable to bind the MEK5 PB1 domain or expression of the p67phox PB1 domain has no effect on ERK5 activation. These findings demonstrate that the PB1 domain mediates the association of MEKK2 and MEKK3 with MEK5 and that the respective PB1 domains of these kinases are critical for regulation of the ERK5 pathway. The free PB1 domain of MEKK2 or MEKK3 functions effectively to inhibit the ERK5 pathway but not the p38 or JNK pathways, demonstrating the specific and unique requirement of the MEKK2 and MEKK3 PB1 domain in regulating ERK5 activation.
A perfused in vitro human iPSC-derived blood–brain barrier faithfully mimics transferrin receptor-mediated transcytosis of therapeutic antibodies
Creators: name: Burgio, Floriana
name: Gaiser, Carine
name: Brady, Kevin
name: Gatta, Viviana
name: Class, Reiner
name: Schrage, Ramona
name: Suter-Dick, Laura
Publisher: name: Springer
Year: 2023
Subjects: subject: 500 - Naturwissenschaften und Mathematik

description: Delivering biologics to elicit a therapeutic response in the central nervous system (CNS) remains challenging due to the presence of the blood brain barrier (BBB). Receptor-mediated transcytosis is a strategy to improve brain exposure after systemic drug administration. The availability of a clinically relevant in vitro BBB model is crucial to investigate transcytosis pathways and to predict the penetration of biologics into the CNS. We created a perfused human in vitro BBB model made of induced pluripotent stem cells (iPSC)-derived brain microvascular endothelial cells (BMEC) for studying transferrin receptor-mediated transcytosis. iPSC-derived BMEC were seeded in the top channel of a three-lane microfluidic device (OrganoPlate®). After 2 days in culture, the established cell model exhibited relevant BBB features, including physiological transendothelial electrical resistance in a transwell setting (1500 Ω*cm), reduced apparent permeability (Papp) to the fluorescence tracer Lucifer yellow (20-fold less than cell-free chips), expression of key BBB markers such as tight junctions proteins, transporters, receptors and functional P-gp efflux pump. Moreover, the model exhibited functional transferrin receptor-mediated uptake and transcytosis. To assess selective transferrin receptor-mediated transcytosis, a mixture of anti-human transferrin receptor (MEM-189) and control (sheep IgG anti-bovine serum albumin) antibodies was perfused in the top channel for 2 h. The Papp of MEM-189 was 11-fold higher than that of the control antibody, demonstrating facilitated receptor-mediated transcytosis. Compared to published work reporting a 2-fold ratio, this result is remarkable and establishes the suitability of our model for exploring receptor-mediated transcytosis and screening of antibodies for putative brain shuttle application. A perfused in vitro human model made of iPSC-derived BMEC with the chief characteristics (barrier tightness, functionality) of the human BBB can be applied to study transferrin receptor (TfR)-mediated transcytosis of therapeutic antibodies. This may bring critical advances in drug shuttle technology. Graphical abstract generated with biorender.com.
(1710) Thomas Freeman Firth
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Purification and Characterization of Three Members of the Photolyase/Cryptochrome Family Blue-light Photoreceptors from Vibrio cholerae
Creators: name: Berrocal-Tito, Gloria
name: Sancar, Aziz
name: Kavakli, I. Halil
name: Bondo, Bruce E.
name: Worthington, Erin N.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2003

description: The sequence of Vibrio cholerae genome revealed three genes belonging to the photolyase/cryptochrome blue-light photoreceptor family. The proteins encoded by the three genes were purified and characterized. All three proteins contain folate and flavin cofactors and have absorption peaks in the range of 350-500 nm. Only one of the three, VcPhr, is a photolyase specific for cyclobutane pyrimidine dimers. The other two are cryptochromes and were designated VcCry1 and VcCry2, respectively. Mutation of phr abolishes photoreactivation of UV-induced killing, whereas mutations in cry1 and cry2 do not affect photorepair activity. VcCry1 exhibits some unique features. Of all cryptochromes characterized to date, it is the only one that contains stoichiometric amounts of both chromophores and retains its flavin cofactor in the two-electron reduced FADH2 form. In addition, VcCry1 exhibits RNA binding activity and co-purifies with an RNA of 60-70 nucleotides in length.
Supplementary Table 4 from α-Amylase immobilization on amidoximated acrylic microfibres activated by cyanuric chloride.
Creators: name: Yaaser Q. Almulaiky
name: Aqlan, Faisal M.
name: Musab Aldhahri
name: Baeshen, Mohammed
name: Khan, Tariq Jamal
name: Khan, Khalid A.
name: Afifi, Mohamed
name: AL-Farga, Ammar
name: Mohiuddin Khan Warsi
name: Alkaled, Mohammed
name: Alayafi, Aisha A. M.
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: Biochemistry
subject: 90301 Biomaterials
subject: FOS: Medical engineering

description: Inhibitor effects on soluble and immobilized α-amylase.
Effects of contaminants detected in animal biological indicators of Sicily on the zebrafish's genome
Creators: name: Vella, Antonio
name: Macaluso, Andrea
name: Cammilleri, Gaetano
name: Collura, Rosaria
name: Pantano, Licia
name: Graci, Stefania
name: Buscemi, Maria Drussilla
name: Calvaruso, Enza
name: Giaccone, Vita
name: Lo Dico, Gianluigi Maria
name: Cicero, Antonello
name: Giangrosso, Giuseppe
name: Germanà, Antonino
name: Piccione, Giuseppe
name: Ferrantelli, Vincenzo
Publisher: name: INFN Open Access Repository
Year: 2017

description: The research aimed to obtain data on the presence of toxic substances in dairy surrounding the Sicilian risk areas and to analyse the in vivo effects of these toxic agents in embryos and larvae of zebrafish. We examined 368 milk samples from cattle and sheep farms (50 for dioxins and 318 for heavy metals detection, respectively). The analysis of heavy metals has provided the development of an ICP-MS method. The dioxins levels were assessed by a HRGC/HRMS method. The results on milk samples revealed a significantly greater presence of dioxins in sheep milk, with average values near the limit of the Reg. EC 1259/2011. Ten samples that came from farms close to Bellolampo dump have detected concentrations greater than EC Reg. 1259/2011 limits. Only six samples of sheep milk have detected Pb concentrations over the LOD but below the limits imposed by the EC Reg. 1881/2006. The highest concentrations of dioxin detected in milk samples studied lead to a decrease in the survival of the larvae and malformations of the skeletal and circulatory systems. Furthermore, an over-expression of genes involved in phenomena of environmental stress such as hsp70, gadd45b, ATF3 CYP1A was found. The data obtained in this study confirm the close relationship between environmental factors and health effects of the population.
Continuous NHANES survey data for environmental ambient and occupational hazard identification—feasibility and preliminary findings for osteoporosis and kidney disease
Creators: name: Park, Robert M.
name: An, Yu
Publisher: name: Taylor & Francis
Year: 2022
Subjects: subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Physiology
subject: Pharmacology
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: Immunology
subject: FOS: Clinical medicine
subject: Marine Biology
subject: Science Policy

description: The Continuous NHANES Survey provides detailed health and environmental chemical burden information on the U.S. population. As of 2012, there were data for 72,000 participants. Based on single biomarker determinations, cumulative burdens were estimated. Because age distributions would differ comparing ambient environmental and occupational exposures, a procedure to distinguish ambient from likely occupational exposures was applied. Associations are reported for osteoporosis and kidney disease-related outcomes with cadmium, lead, and other metals. Cumulative cadmium burden (from blood cadmium, ambient and occupational) was a strong predictor of bone fracture risk and ambient tungsten also had a positive association. Cumulative lead (ambient and occupational) had a negative (“protective”) association with fractures as did mercury (occupational). Bone mineral density was statistically significant and similarly predicted by metal exposures. Kidney disease was significantly associated with cumulative lead burdens from both the estimated ambient and occupational sources and with ambient blood cadmium but was most strongly associated with cumulative occupational uranium burden. Systolic blood pressure statistically significantly increased with cumulative ambient and occupational lead (blood) burden and with ambient cadmium and cobalt. Diastolic blood pressure was significantly associated with several cadmium and cobalt metrics along with ambient and occupational cumulative burdens for lead. For environmental substances with burden half-lives measured in years, NHANES offers opportunities for hypothesis generation and confirmation.
Étude et correction de l’effet des électrodes pour les mesures TRE à petite échelle et application
Creators: name: Verdet, Cécile
name: Sirieix, Colette
name: Anguy, Yannick
name: Gaborieau, Cécile
name: Clément, Rémi
Publisher: name: Academic Journal of Civil Engineering
Year: 2021

description: La tomographie de résistivité électrique (TRE) est une méthode géophysique permettant d'estimer indirectement les variations du degré de saturation des matériaux. On adapte cette méthode pour un diagnostic par imagerie électrique à petite échelle d’une paroi naturelle ou bâtie. On montre que la taille finie des électrodes impacte la mesure électrique quand le ratio enfoncement / espacement des électrodes est supérieur à 20%. On corrige cet effet fallacieux via une inversion des résistances de transfert mesurées en surface avec une électrode équivalente ponctuelle (EEP) placée en profondeur à 73% de la longueur de l’électrode. Cette correction est appliquée à des mesures de micro-TRE à la paroi d’une carrière calcaire. On peut ainsi suivre les mouvements d’eau dans la paroi durant plusieurs mois, avant et après des feux, démontrant un impact durable de ces feux sur les premiers centimètres de la roche.
description: Academic Journal of Civil Engineering, Vol 38 No 2 (2021): Special Issue - Diagnobéton 2021
RASSF2 Is a Novel K-Ras-specific Effector and Potential Tumor Suppressor
Creators: name: Taylor, Barbara J.
name: Elam, Candice
name: Clark, Geoffrey J.
name: Ülkü, Aylin S.
name: Ellis, Chad A.
name: Vos, Michele D.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2003

description: Ras proteins regulate a wide range of biological processes by interacting with a broad assortment of effector proteins. Although activated forms of Ras are frequently associated with oncogenesis, they may also provoke growth-antagonistic effects. These include senescence, cell cycle arrest, differentiation, and apoptosis. The mechanisms that underlie these growth-inhibitory activities are relatively poorly understood. Recently, two related novel Ras effectors, NORE1 and RASSF1, have been identified as mediators of apoptosis and cell cycle arrest. Both of these proteins exhibit many of the properties normally associated with tumor suppressors. We now identify a novel third member of this family, designated RASSF2. RASSF2 binds directly to K-Ras in a GTP-dependent manner via the Ras effector domain. However, RASSF2 only weakly interacts with H-Ras. Moreover, RASSF2 promotes apoptosis and cell cycle arrest and is frequently down-regulated in lung tumor cell lines. Thus, we identify RASSF2 as a new member of the RASSF1 family of Ras effectors/tumor suppressors that exhibits a specificity for interacting with K-Ras.
Supplementary Table 2 from α-Amylase immobilization on amidoximated acrylic microfibres activated by cyanuric chloride.
Creators: name: Yaaser Q. Almulaiky
name: Aqlan, Faisal M.
name: Musab Aldhahri
name: Baeshen, Mohammed
name: Khan, Tariq Jamal
name: Khan, Khalid A.
name: Afifi, Mohamed
name: AL-Farga, Ammar
name: Mohiuddin Khan Warsi
name: Alkaled, Mohammed
name: Alayafi, Aisha A. M.
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: Biochemistry
subject: 90301 Biomaterials
subject: FOS: Medical engineering

description: substrate specificity of soluble and immobilized α-amylase.
Evaluation of Anisakis presence in fish products of Sicily and survey on the efficiency of storage methods on the viability and allergy potential
Creators: name: Cammilleri, Gaetano
name: Costa, Antonella
name: Vella, Antonio
name: Macaluso, Andrea
name: Graci, Stefania
name: Buscemi, Maria Drussilla
name: Cassata, Giovanni
name: Migliazzo, Aldo
name: Giuffrida, Alessandro
name: Ferrantelli, Vincenzo
Publisher: name: INFN Open Access Repository
Year: 2017

description: The research aimed to evaluate the prevalence, geographical distribution and seasonal occurrence of Anisakidae nematodes infestation in fish products marketed in Sicily. A study on mercury detection was also conducted in order to evaluate a difference in the accumulation between parasitized and not parasitized fishes. We examined 1419 fish samples for Anisakidae larvae detection by visual inspection and digestion method. The collected larvae were identified at genus level by optical microscopy and at species level by molecular methods (PCR-RFLP and multiplex- PCR). A Real Time PCR was carried out for the assessment of DNA of Anisakidae presence in 23 transformed fish products. The method showed high sensitivity and specificity for the scope. The results obtained revealed the presence of Anisakidae larve in 463 fish samples (32,6%) and of Anisakidae DNA in 3 trasformed fish products (13%). All the larvae detected in transformed fish products were dead, with absence of motility until 24 h. Molecular analysis confirm Anisakis pegreffii as the prevalent species of Mediterranean Sea. Furthermore, the presence of A. simplex s.s., A. physeteris, A. pegreffii/A. simplex s.s. hybrid forms and Hysterothylacium fabri was verified with lower prevalence values. A significant correlation was found between Anisakidae infestation and presence of heavy metals (Hg) levels (p<0.05).
Monitoring of ultrasonic pulse velocity for earth concrete properties evaluation - Effect of curing conditions
Creators: name: Saliba, Jacqueline
name: SBARTAÏ, Zoubir Mehdi
name: SAIYOURI, Nadia
name: NGO, Duc Chinh
Publisher: name: Academic Journal of Civil Engineering
Year: 2021

description: This paper aims to study the effect of curing conditions on the mechanical properties of earth concrete. The studied earth concrete mixtures were constituted of different percentages of clayey and sandy soil with limited quantities of cement, lime and hemp fibers. Earth concrete mixtures have been cast and vibrated as normal concrete. Compression tests have been carried out on samples at different age and curing conditions. The ultrasonic non-destructive technique has been used for monitoring the hardening of soil concrete in function of the curing conditions. The results show that the Ultrasonic Pulse Velocity (UPV) decreases with the addition of clayey soil and hemp fibers. In addition, a good correlation has been observed between the compressive strength and the UPV and thus UPV can be used as an indicator for the evaluation of earth concrete mechanical properties.
description: Academic Journal of Civil Engineering, Vol 38 No 2 (2021): Special Issue - Diagnobéton 2021
Supplementary Figure 5 from α-Amylase immobilization on amidoximated acrylic microfibres activated by cyanuric chloride
Creators: name: Yaaser Q. Almulaiky
name: Aqlan, Faisal M.
name: Musab Aldhahri
name: Baeshen, Mohammed
name: Khan, Tariq Jamal
name: Khan, Khalid A.
name: Afifi, Mohamed
name: AL-Farga, Ammar
name: Mohiuddin Khan Warsi
name: Alkaled, Mohammed
name: Alayafi, Aisha A. M.
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: Biochemistry
subject: 90301 Biomaterials
subject: FOS: Medical engineering

description: Effect of pH, temperature, km
Regulation of the Epithelial Na + Channel by Cytosolic ATP
Creators: name: Marunaka, Yoshinori
name: Ishikawa, Toru
name: Jiang, Chong
name: Stutts, M. Jackson
name: Rotin, Daniela
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2003

description: The epithelial Na+ channel (ENaC), composed of three subunits (alphabetagamma), is expressed in various Na(+)-absorbing epithelia and plays a critical role in salt and water balance and in the regulation of blood pressure. By using patch clamp techniques, we have examined the effect of cytosolic ATP on the activity of the rat alphabetagammaENaC (rENaC) stably expressed in NIH-3T3 cells and in Madin-Darby canine kidney epithelial cells. The inward whole-cell current attributable to rENaC activity ran down when these cells were dialyzed with an ATP-free pipette solution in the conventional whole-cell voltage-clamping technique. This run down was prevented by 2 mM ATP (but not by AMP or ADP) in the pipette solution or by the poorly or non-hydrolyzable analogues of ATP (adenosine 5'-O-(thiotriphosphate) and adenosine 5'-(beta,gamma-imino)triphosphate) in both cell lines, suggesting that protection from run down was mediated through non-hydrolytic nucleotide binding. Accordingly, we demonstrate binding of ATP (but not AMP) to alpharENaC expressed in Madin-Darby canine kidney cells, which was inhibited upon mutation of the two putative nucleotide-binding motifs of alpharENaC. Single channel analyses indicated that the run down of currents observed in the whole-cell recording was attributable to run down of channel activity, defined as NPo (the product of the number of channels and open probability). We propose that this novel ATP regulation of ENaC may be, at least in part, involved in the fine-tuning of ENaC activity under physiologic and pathophysiologic conditions.