Resitin and adiponectin in the Bahraini population : plasma levels, gene polymorphisms and their association with type 2 diabetes and related metabolic parameters.
Creators: name: Hannan, Fatima Al
Publisher: name: Royal College of Surgeons in Ireland
Year: 2019
Subjects: Uncategorized

description: <strong>Low concentrations of adiponectin, the protein product of the adiponectin </strong><strong>gene (ADIPOQ), and high concentrations of resistin have been reported </strong><strong>to be associated with obesity and insulin resistance. Several adiponectin </strong><strong>gene polymorphisms have been described, and their association with </strong><strong>obesity, type 2 diabetes mellitus (T2DM), and metabolic syndrome, in </strong><strong>different populations and sample types, is controversial. The purpose of </strong><strong>the present study was to investigate the association of the two most well-known single nucleotide polymorphisms (SNPs) of ADIPOQ (+45T&gt;G </strong><strong>and +276G&gt;T) with serum adiponectin concentrations, metabolic </strong><strong>parameters associated with diabetes, and risk of T2DM in the Bahraini </strong><strong>population. We also aimed to investigate the associations between </strong><strong>serum adiponectin and resistin levels with the metabolic parameters and </strong><strong>the prevalence of T2DM in Bahraini population.</strong> <strong></strong><strong>We performed a cross-sectional study using a representative sample of </strong><strong>140 unrelated Bahraini patients with T2DM and 66 non-diabetic healthy </strong><strong>subjects. The polymerase chain reaction-restriction fragment length </strong><strong>polymorphism (PCR-RFLP) method was used to determine the </strong><strong>distribution of allele and genotype frequency of the SNP +45T&gt;G </strong><strong>polymorphism (exon2) and SNP +276G&gt;T polymorphism (intron 2) in </strong><strong>ADIPOQ. Lipid profile was measured by enzymatic methods. An ELISA </strong><strong>was used to determine serum adiponectin and resistin levels.</strong> <strong></strong><strong>It was observed that obesity, insulin resistance and T2DM are associated </strong><strong>with low serum adiponectin levels. Compared with the control group, the </strong><strong>T2DM group exhibited lower adiponectin levels and higher resistin levels. </strong><strong>The G allele and TG/GG genotype of SNP +45T&gt;G occurred more </strong><strong>frequently than the common T allele and TT genotype in T2DM patients </strong><strong>compared to the controls (P<strong>SNP +45T&gt;G were associated with lower serum adiponectin levels. </strong><strong>There was no statistically significant difference in allele and genotype </strong><strong>frequencies of SNP +276G&gt;T comparing control group with T2DM group.</strong></strong> <strong></strong><strong>No association with metabolic parameters was detected with either of the </strong><strong>SNPs. In summary, our results demonstrated that, adiponectin SNP </strong><strong>+45T&gt;G, rather than SNP +276G&gt;T is more associated with adiponectin </strong><strong>levels. However, we could not confirm an association of these two SNPs </strong><strong>with metabolic parameters of the metabolic syndrome.</strong>