Dasatinib and Quercetin Short-term simultaneous administration improves physical capacity in human
Creators: name: Tkemaladze, Jaba
Publisher: name: figshare
Year: 2021
Subjects: subject: Health Care
subject: 90399 Biomedical Engineering not elsewhere classified
subject: FOS: Medical engineering
subject: FOS: Medical engineering
subject: 110308 Geriatrics and Gerontology
subject: FOS: Clinical medicine
subject: FOS: Clinical medicine

description: Participants The experiment included middle aged and elderly men between the ages of 36-60. Average age was 53, 1years. The majority of them led sedentary lifestyle, others were slightly active, some were smokers, some moderately drank alcohol. The participants did not have any specific illnesses that potentially could affect the experiment. These 64 participants were equally divided into 4 different groups. Such as D+Q, D+P, Q+P and P+P. D+Q group orally administered 50mg dasatinib with 500mg quercetin. D+P group orally administered dasatinib along with placebo. Q+P group took 500mg quercetin with 50mg placebo. P+P group orally administered two compounds of placebo with dosage of 500mg and 50mg accordingly. All participants provided informed consent. The whole research was conductedin accordance with the Helsinki Declaration. Reagents Dasatinib is an inhibitor of multiple tyrosine kinases, used for treating cancers, and is known to interfere with EFNB-dependent suppression of apoptosis. Dasatinib (50 mg compound) was obtained from LC Laboratories [13]. Quercetin is a natural flavonol, inhibits PI3K, other kinases, and serpines. Quercetin (500 mg compound) were obtained from SigmaAldrich Co. (St. Louis, MO, USA; catalog number Q4951) [14,15]. Statistical Analysis All data were presented as mean ± standard deviation. The values between groups for the dependent variables were analyzed to determine if the distributions were normal using Kolmogorov-Smirnov (K-S) Normality test. Comparison between pre- and post-exercise was performed through the Paired-Samples T test procedure in order to examine the differences between tests. It was also used for comparing the differences for pre-test ME and EE and post-tests ME and EE. Significance level was set at α = 0.05 and 0.01. The statistical package program was used to evaluate the results of the study. Medical Screening A medical screening consisting of complete blood count, C-reactive protein and creatinine blood test were performed on participants a day prior and 21 days after the administration of compounds. Complete blood count covered all basic parameters. Venous blood from participants was collected into anti-coagulant EDTA tubes and differential full blood counts were determined on a Beckman Coulter. C-reactive protein concentrations and creatinine blood test were determined on a Beckman auto-analyser. Stair Ascending Test (SAT) For the accurate assessment of senolytic effect of all compounds stair ascending test was done by participants a day prior to the start of trial and 21 days after the end of trial along with medical screening each time. SAT was performed at a randomly-selected block of flats. Participants were required to ascend 4 floors [16]. The total vertical distance covered in climbing the 4 floors was 12.48 metres. This vertical distance covered was based on the number of steps climbed and summed up as follows: Height of a step = 13 cm Number of steps per flight: 12 steps Number of flights= 8. Therefore, vertical height of 4 floors= (13 cm x 12 steps) x 8 flights) = 1248 cm = 12.48 m. Participants was clearly acquainted with SAT requirements in advance. They needed to ascend at rapid, but even pace during the whole test and also to take only one step at a time. They went through a standardized warm-up protocol that consisted of five minutes of stretching for the quadriceps, hamstrings and calf muscles. Exercise attire was mandatory. Namely, shorts, t-shirts and sports shoes. A five-minute rest interval was initiated between the standardized warm-up and the SAT. Before the warm-up the systolic and diastolic blood pressures of participants were measured once. Then right away after reaching 4 floors we measured their systolic and diastolic blood pressures 4 more times with 10 minutes of interval between each. Overall the whole process took about 40-45 minutes.