title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ SOS-Ð¾ÑÐ²ÐµÑÐ° Ð½Ð° Ð¿ÑÐ¾ÑÐµÑÑ Ð´ÐµÐ»ÐµÐ½Ð¸Ñ Escherichia coli
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Influence of the SOS response on the Escherichia coli division process
Creators: name: Ð ÑÐ¼ÑÐ½ÑÐµÐ²Ð°, ÐÐ°ÑÐ°Ð»ÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÐÐ¸Ð¾ÑÐ¸Ð·Ð¸ÐºÐ°
ÐÐ»ÐµÑÐºÐ° (Ð±Ð¸Ð¾Ð».)
ÑÐµÐºÐ²ÐµÑÑÑÐ°ÑÐ¸Ñ
ÐºÑÐ¿Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
ÑÐ¸ÑÑÐµÐ¼Ð° Ð½ÑÐºÐ»ÐµÐ¾Ð¸Ð´Ð½Ð¾Ð¹ Ð¾ÐºÐºÐ»ÑÐ·Ð¸Ð¸
sequestration
capping
nucleoid occlusion

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«ÐÐ»Ð¸ÑÐ½Ð¸Ðµ SOS-Ð¾ÑÐ²ÐµÑÐ° Ð½Ð° Ð¿ÑÐ¾ÑÐµÑÑ Ð´ÐµÐ»ÐµÐ½Ð¸Ñ Escherichia coliÂ». ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð²Ð·Ð°Ð¸Ð¼Ð¾ÑÐ²ÑÐ·Ð¸ SOS-Ð¾ÑÐ²ÐµÑÐ° Ñ Ð¿ÑÐ¾ÑÐµÑÑÐ¾Ð¼ Ð´ÐµÐ»ÐµÐ½Ð¸Ñ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ E. coli. ÐÐ»Ñ ÑÑÐ¾Ð³Ð¾ Ð±ÑÐ»Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ñ ÑÑÐ°Ð¼Ð¼Ñ Ñ Ð´ÐµÐ»ÐµÑÐ¸ÑÐ¼Ð¸ Ð¸ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ðµ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ð¸ Ð´Ð»Ñ ÐºÐ¾Ð½ÑÑÐ¾Ð»Ñ ÑÑÐ¾Ð²Ð½Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð±ÐµÐ»ÐºÐ° SulA. Ð ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð±ÑÐ»Ð¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ ÑÑÐ°Ð½Ð´Ð°ÑÑÐ½ÑÐµ Ð¼ÐµÑÐ¾Ð´Ñ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾Ð¹ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ð¸, ÑÑÐ°Ð´Ð¸ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð°Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ, ÑÐ²ÐµÑÑÑÐ°Ð·ÑÐµÑÐ°ÑÑÐ°Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¿Ð¾Ð»ÑÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²ÐµÐ½Ð½ÑÐ¹ Ð¸Ð¼Ð¼ÑÐ½Ð¾Ð±Ð»Ð¾ÑÑÐ¸Ð½Ð³ Ð´Ð»Ñ Ð¸Ð·Ð¼ÐµÑÐµÐ½Ð¸Ñ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸. Ð ÑÐ¾Ð´Ðµ ÑÐ°Ð±Ð¾ÑÑ ÐºÐ¾ÑÐ²ÐµÐ½Ð½ÑÐ¼ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð±ÑÐ»Ð° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð¾ÑÐµÐ½ÐºÐ° ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ Ð±ÐµÐ»ÐºÐ° SulA Ð² ÐºÐ»ÐµÑÐºÐ°Ñ Ð²Ð¾ Ð²ÑÐµÐ¼Ñ SOS-Ð¾ÑÐ²ÐµÑÐ°. Ð ÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð³Ð¾Ð²Ð¾ÑÑÑ Ð¾ ÑÐ¾Ð¼, ÑÑÐ¾ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ñ SulA Ð² Ð½ÐµÑÐºÐ¾Ð»ÑÐºÐ¾ ÑÐ°Ð· Ð¼ÐµÐ½ÑÑÐµ, ÑÐµÐ¼ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ñ FtsZ Ð² ÑÐµÑ Ð¶Ðµ ÐºÐ»ÐµÑÐºÐ°Ñ. ÐÑÐ¾Ð¼Ðµ ÑÐ¾Ð³Ð¾, Ð±ÑÐ»Ð° Ð¸Ð·ÑÑÐµÐ½Ð° ÑÐ¾Ð»Ñ ÑÐ¸ÑÑÐµÐ¼ Ð¿Ð¾Ð·Ð¸ÑÐ¸Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Z-ÐºÐ¾Ð»ÑÑÐ° Ð¿ÑÐ¸ Ð²Ð¾ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¸Ð¸ Ð´ÐµÐ»ÐµÐ½Ð¸Ñ, Ð² ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð±ÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Min-ÑÐ¸ÑÑÐµÐ¼Ð° Ð¸Ð³ÑÐ°ÐµÑ ÐºÐ»ÑÑÐµÐ²ÑÑ ÑÐ¾Ð»Ñ Ð² ÑÑÐ¾Ð¼ Ð¿ÑÐ¾ÑÐµÑÑÐµ. ÐÐ°Ð½Ð½ÑÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð³Ð¾Ð²Ð¾ÑÑÑ Ð¾ ÑÐ¾Ð¼, ÑÑÐ¾ Ð¾ÑÐ½Ð¾Ð²Ð°Ð½Ð½Ð°Ñ Ð½Ð° ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ñ in vitro Ð¼Ð¾Ð´ÐµÐ»Ñ ÑÐµÐºÐ²ÐµÑÑÑÐ°ÑÐ¸Ð¸ Ð½Ðµ Ð¾Ð±ÑÑÑÐ½ÑÐµÑ Ð¿ÑÐ¾ÑÐµÑÑ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¿Ð¾Ð´ Ð´ÐµÐ¹ÑÑÐ²Ð¸ÐµÐ¼ SulA Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ in vivo. ÐÐ¾Ð²ÑÐµ Ð´Ð°Ð½Ð½ÑÐµ Ð¾ ÑÐ¾Ð»Ð¸ ÑÐ¸ÑÑÐµÐ¼ Ð¿Ð¾Ð·Ð¸ÑÐ¸Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¿ÑÐ¸ Ð²Ð¾ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¸Ð¸ Ð´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¼Ð¾Ð³ÑÑ Ð¿Ð¾Ð¼Ð¾ÑÑ Ð¿Ð¾Ð½ÑÑÑ, ÐºÐ°Ðº Ð±Ð°ÐºÑÐµÑÐ¸ÑÐ¼ ÑÐ´Ð°ÐµÑÑÑ Ð¿ÐµÑÐµÐ¶Ð¸Ð²Ð°ÑÑ Ð½ÐµÐ±Ð»Ð°Ð³Ð¾Ð¿ÑÐ¸ÑÑÐ½ÑÐµ ÑÑÐ»Ð¾Ð²Ð¸Ñ, Ð½Ð°Ð¿ÑÐ¸Ð¼ÐµÑ, ÑÐµÑÐ°Ð¿Ð¸Ñ Ð°Ð½ÑÐ¸Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÐ¼Ð¸ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ°Ð¼Ð¸.
description: The subject of the graduate qualification work is âInfluence of the SOS response on the Escherichia coli division processâ. The given work is devoted to study relationship between the SOS response and the cell division process of E. coli. For this, strains with several gene deletions and genetic constructions for control of SulA level were created. Standard methods of molecular biology, conventional fluorescence microscopy, super-resolution microscopy, and semi-quantitative Western blotting for concentration measurements were used. During the work an indirect method was used to estimate the concentration of the SulA protein in cells during the SOS response. The results indicate that the SulA concentration was several times lower than the FtsZ concentration in the same cells. In addition, the role of Z-ring positioning systems in the cell division recovery was studied and it was shown that the Min-system plays a key role in this process. These results indicate that the sequestration model based on in vitro experiments does not explain the process of division inhibition by SulA in vivo. Also, new knowledge about the role of positioning systems in the cell division recovery could help to understand how bacteria are able to survive under adverse conditions, such as antibiotic therapy.