The cuticular wax compositions and crystal coverage of leaves and petals differ in a consistent way between plant species: supplementary data and analytical code
Creators: name: Tunstad, Sverre Aarseth
name: D. Bull, Ian
name: Rands, Sean
name: Whitney, Heather
Publisher: name: figshare
Year: 2024
Subjects: classificationCode: 310410
subject: Phylogeny and comparative analysis
classificationCode: 310802
subject: Plant biochemistry
classificationCode: 310806
subject: Plant physiology
classificationCode: 340101
subject: Analytical spectrometry

description: Supplementary datasets and analysis code supporting '<i>The cuticular wax compositions and crystal coverage of leaves and petals differ in a consistent way between plant species</i>' by Sverre Aarseth Tunstad, Ian D. Bull, Sean A. Rands and Heather M. Whitney (Open Biology <b>14</b>: 230430 https://doi.org/10.1098/rsob.230430)<br>SUPPLEMENTARY_DATA_total_wax_amounts.txt, csv:The table consists of eight columns. "Plant_ug_cm2" represents the total amount of waxes that was extracted from in each sample, divided by the total surface area. "Crystals" refers to the presence or absence of crystals on each sample. "Yes" or "No" refers to coverage on both sides, "Half" means only one side was covered in wax-crystals.SUPPLEMENTARY_DATA_compound_classes.txt, csv:The table consists of 12 columns."Compound_class" contains the "Plant_ug_cm2" is the same as above. "Compound_class_ug_cm2" describes the total sum of each compound per class (e.g. tetracosanol, hexacosanol and octacosanol in "Primary alcohols") per cm2. "Compound_class_percentage_of_total" is the percentage which each compound class makes up of the total wax coverage.SUPPLEMENTARY_DATA_ACL.txt, csv:The table consists of 12 columns. "ACL" refers to the Average Chain Length, for each aliphatic compound class found in each sample. For our analysis, we only considered the five main ones (primary alcohols, aldehydes, fatty acids, esters and alkanes), but all aliphatic compounds have been included in this table.<br>'<i>wax_analysis_v2.r</i>' is a text file with the detail of the <i>R</i> analysis used in 'The cuticular wax compositions and crystal coverage of leaves and petals[ST1] differ in a consistent manner across 49 plant species' by Tunstad et al. - annotations are given throughout the text<br>The zipped file '<i>processed_datafiles_used_in_analysis.zip'</i> contains 14 files, which are either text files containing taxonomic detail necessary for generating the phylogenetic trees, or else rearrangements or processed versions of the main data files (see main unzipped files in this figshare directory), presented in a format suitable for the various packages called within the <i>R</i> code presented within<i> wax_analysis.r</i>.Tidied comma-delimited file containing relevant wax data by species formatted for <i>R</i> analysis* wax_data.csvSpecies phylogenetic detail needed to generate tree* wax_species.csvRearranged data tables arranging the leaf and petal compounds for analysis* leaf_chemistry_actual_table.txt* petal_chemistry_actual table.txtTen additional files: five containing the processed average carbon chain lengths of the major long-chain compounds in the leaves and petals of species, and five accompanying files that list necessary phylogenetic detail for tree calculation* chain_length_aldehyde.txt* chain_length_alkane.txt* chain_length_ester.txt* chain_length_fatty_acid.txt* chain_length_primary_alcohol.txt* chain_species_aldehyde.txt* chain_species_alkane.txt* chain_species_ester.txt* chain_species_fatty_acid.txt* chain_species_primary_alcohol.txt<br><b>NOTE ON CHANGES FROM VERSION 1: In response to reviewer suggestions, we have altered the analysis slightly, by adding normality tests and conducting tests on logged data. The code presented in </b><i>wax_analysis_v2.r</i> <b>reflects these changes. The other four data files have not been changed.</b>