Pitfalls in assessing stromal tumor infiltrating lymphocytes (sTILs) in breast cancer
Creators: name: Kos, Zuzana
name: Roblin, Elvire
name: Kim, Rim S.
name: Michiels, Stefan
name: Gallas, Brandon D.
name: Chen, Weijie
name: Van De Vijver, Koen K.
name: Goel, Shom
name: Adams, Sylvia
name: Demaria, Sandra
name: Viale, Giuseppe
name: Nielsen, Torsten O.
name: Badve, Sunil S.
name: Symmans, W. Fraser
name: Sotiriou, Christos
name: Rimm, David L.
name: Hewitt, Stephen
name: Denkert, Carsten
name: Loibl, Sibylle
name: Luen, Stephen J.
name: Bartlett, John M. S.
name: Savas, Peter
name: Pruneri, Giancarlo
name: Dillon, Deborah A.
name: Cheang, Maggie Chon U.
name: Tutt, Andrew
name: Hall, Jacqueline A.
name: Kok, Marleen
name: Horlings, Hugo M.
name: Madabhushi, Anant
name: Van Der Laak, Jeroen
name: Ciompi, Francesco
name: Laenkholm, Anne-Vibeke
name: Bellolio, Enrique
name: Gruosso, Tina
name: Fox, Stephen B.
name: Araya, Juan Carlos
name: Floris, Giuseppe
name: Hudeček, Jan
name: Voorwerk, Leonie
name: Beck, Andrew H.
name: Kerner, Jen
name: Larsimont, Denis
name: Declercq, Sabine
name: Van Den Eynden, Gert
name: Pusztai, Lajos
name: Ehinger, Anna
name: Yang, Wentao
name: AbdulJabbar, Khalid
name: Yuan, Yinyin
name: Singh, Rajendra
name: Hiley, Crispin
name: Bakir, Maise Al
name: Lazar, Alexander J.
name: Naber, Stephen
name: Wienert, Stephan
name: Castillo, Miluska
name: Curigliano, Giuseppe
name: Dieci, Maria-Vittoria
name: André, Fabrice
name: Swanton, Charles
name: Reis-Filho, Jorge
name: Sparano, Joseph
name: Balslev, Eva
name: Chen, I-Chun
name: Stovgaard, Elisabeth Ida Specht
name: Pogue-Geile, Katherine
name: Blenman, Kim R. M.
name: Penault-Llorca, Frédérique
name: Schnitt, Stuart
name: Lakhani, Sunil R.
name: Vincent-Salomon, Anne
name: Rojo, Federico
name: Braybrooke, Jeremy P.
name: Hanna, Matthew G.
name: Soler-Monsó, M. Teresa
name: Bethmann, Daniel
name: Castaneda, Carlos A.
name: Willard-Gallo, Karen
name: Sharma, Ashish
name: Lien, Huang-Chun
name: Fineberg, Susan
name: Thagaard, Jeppe
name: Comerma, Laura
name: Gonzalez-Ericsson, Paula
name: Brogi, Edi
name: Loi, Sherene
name: Saltz, Joel
name: Klaushen, Frederick
name: Cooper, Lee
name: Amgad, Mohamed
name: Moore, David A.
name: Salgado, Roberto
name: Hyytiäinen, Aini
name: Hida, Akira I.
name: Thompson, Alastair
name: Lefevre, Alex
name: Gown, Allen
name: Lo, Amy
name: Sapino, Anna
name: Moreira, Andre M.
name: Richardson, Andrea
name: Vingiani, Andrea
name: Bellizzi, Andrew M.
name: Guerrero, Angel
name: Grigoriadis, Anita
name: Garrido-Castro, Ana C.
name: Cimino-Mathews, Ashley
name: Srinivasan, Ashok
name: Acs, Balazs
name: Singh, Baljit
name: Calhoun, Benjamin
name: Haibe-Kans, Benjamin
name: Solomon, Benjamin
name: Thapa, Bibhusal
name: Nelson, Brad H.
name: Ballesteroes-Merino, Carmen
name: Criscitiello, Carmen
name: Boeckx, Carolien
name: Colpaert, Cecile
name: Quinn, Cecily
name: Chennubhotla, Chakra S.
name: Solinas, Cinzia
name: Drubay, Damien
name: Sabanathan, Dhanusha
name: Peeters, Dieter
name: Zardavas, Dimitrios
name: Höflmayer, Doris
name: Johnson, Douglas B.
name: Thompson, E. Aubrey
name: Perez, Edith
name: ElGabry, Ehab A.
name: Blackley, Elizabeth F.
name: Reisenbichler, Emily
name: Chmielik, Ewa
name: Gaire, Fabien
name: Lu, Fang-I
name: Azmoudeh-Ardalan, Farid
name: Peale, Franklin
name: Hirsch, Fred R.
name: Acosta-Haab, Gabriela
name: Farshid, Gelareh
name: Broeckx, Glenn
name: Koeppen, Harmut
name: Haynes, Harry R.
name: McArthur, Heather
name: Joensuu, Heikki
name: Olofsson, Helena
name: Cree, Ian
name: Nederlof, Iris
name: Frahm, Isabel
name: Brcic, Iva
name: Chan, Jack
name: Ziai, James
name: Brock, Jane
name: Weseling, Jelle
name: Giltnane, Jennifer
name: Lemonnier, Jerome
name: Zha, Jiping
name: Ribeiro, Joana
name: Lennerz, Jochen K.
name: Carter, Jodi M.
name: Hartman, Johan
name: Hainfellner, Johannes
name: Le Quesne, John
name: Juco, Jonathan W.
name: Van Den Berg, Jose
name: Sanchez, Joselyn
name: Cucherousset, Joël
name: Adam, Julien
name: Balko, Justin M.
name: Saeger, Kai
name: Siziopikou, Kalliopi
name: Sikorska, Karolina
name: Weber, Karsten
name: Steele, Keith E.
name: Emancipator, Kenneth
name: El Bairi, Khalid
name: Allison, Kimberly H.
name: Korski, Konstanty
name: Buisseret, Laurence
name: Shi, Leming
name: Kooreman, Loes F. S.
name: Molinero, Luciana
name: Estrada, M. Valeria
name: Van Seijen, Maartje
name: Lacroix-Triki, Magali
name: Sebastian, Manu M.
name: Balancin, Marcelo L.
name: Mathieu, Marie-Christine
name: Van De Vijver, Mark
name: Rebelatto, Marlon C.
name: Piccart, Martine
name: Goetz, Matthew P.
name: Preusser, Matthias
name: Khojasteh, Mehrnoush
name: Sanders, Melinda E.
name: Regan, Meredith M.
name: Barnes, Michael
name: Christie, Michael
name: Misialek, Michael
name: Ignatiadis, Michail
name: De Maaker, Michiel
name: Van Bockstal, Mieke
name: Harbeck, Nadia
name: Tung, Nadine
name: Laudus, Nele
name: Sirtaine, Nicolas
name: Burchardi, Nicole
name: Ternes, Nils
name: Radosevic-Robin, Nina
name: Gluz, Oleg
name: Grimm, Oliver
name: Nuciforo, Paolo
name: Jank, Paul
name: Kirtani, Pawan
name: Watson, Peter H.
name: Jelinic, Peter
name: Francis, Prudence A.
name: Russell, Prudence A.
name: Pierce, Robert H.
name: Hills, Robert
name: Leon-Ferre, Roberto
name: De Wind, Roland
name: Shui, Ruohong
name: Leung, Samuel
name: Tabbarah, Sami
name: Souza, Sandra C.
name: O’Toole, Sandra
name: Swain, Sandra
name: Dudgeon, Sarah
name: Willis, Scooter
name: Ely, Scott
name: Bedri, Shahinaz
name: Irshad, Sheeba
name: Liu, Shiwei
name: Hendry, Shona
name: Bianchi, Simonetta
name: Bragança, Sofia
name: Paik, Soonmyung
name: Luz, Sua
name: Gevaert, Thomas
name: D’Alfons, Timothy
name: John, Tom
name: Sugie, Tomohagu
name: Kurkure, Uday
name: Bossuyt, Veerle
name: Manem, Venkata
name: Cámaea, Vincente Peg
name: Tong, Weida
name: Tran, William T.
name: Wang, Yihong
name: Allory, Yves
name: Husain, Zaheed
name: Bago-Horvath, Zsuzsanna
Publisher: name: Apollo - University of Cambridge Repository
Year: 2020
Subjects: Article
/631/67/580/1884
/692/53/2422
article

description: Abstract: Stromal tumor-infiltrating lymphocytes (sTILs) are important prognostic and predictive biomarkers in triple-negative (TNBC) and HER2-positive breast cancer. Incorporating sTILs into clinical practice necessitates reproducible assessment. Previously developed standardized scoring guidelines have been widely embraced by the clinical and research communities. We evaluated sources of variability in sTIL assessment by pathologists in three previous sTIL ring studies. We identify common challenges and evaluate impact of discrepancies on outcome estimates in early TNBC using a newly-developed prognostic tool. Discordant sTIL assessment is driven by heterogeneity in lymphocyte distribution. Additional factors include: technical slide-related issues; scoring outside the tumor boundary; tumors with minimal assessable stroma; including lymphocytes associated with other structures; and including other inflammatory cells. Small variations in sTIL assessment modestly alter risk estimation in early TNBC but have the potential to affect treatment selection if cutpoints are employed. Scoring and averaging multiple areas, as well as use of reference images, improve consistency of sTIL evaluation. Moreover, to assist in avoiding the pitfalls identified in this analysis, we developed an educational resource available at www.tilsinbreastcancer.org/pitfalls.