Additional file 1 of Quantitative parameters of lymphocyte nuclear morphology in bronchoalveolar lavage fluid as novel biomarkers for sarcoidosis
Creators: name: Horimasu, Yasushi
name: Yamaguchi, Kakuhiro
name: Sakamoto, Shinjiro
name: Masuda, Takeshi
name: Miyamoto, Shintaro
name: Nakashima, Taku
name: Iwamoto, Hiroshi
name: Fujitaka, Kazunori
name: Hamada, Hironobu
name: Hattori, Noboru
Publisher: name: figshare
Year: 2021
Subjects: subject: 29999 Physical Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: Medicine
subject: Cell Biology
subject: Molecular Biology
subject: Pharmacology
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: FOS: Biological sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics
subject: Developmental Biology
subject: Hematology

description: Additional file 1: Fig. S1. Frequency distribution of BALF lymphocyte nuclear area. The dark gray bars represent the frequency distribution of the BALF lymphocyte nuclear area in sarcoidosis patients, while the light gray bars represent those in the other ILD patients. The vertical dotted line represents the sarcoidosis-diagnostic threshold of the nuclear area (428.1 μm2). Representative lymphocytes with small nuclear areas, those near the threshold value, and those with large nuclear areas are shown in the lower left panel, lower middle panel, and lower right panel, respectively. Fig. S2. Frequency distribution of BALF lymphocyte nuclear perimeters. The dark gray bars represent the frequency distribution of the BALF lymphocyte nuclear perimeters in sarcoidosis patients, while the light gray bars represent those in the other ILD patients. The vertical dotted line represents the sarcoidosis-diagnostic threshold of the nuclear perimeter (71.3 μm). Representative lymphocytes with short nuclear perimeters, those near the threshold value, and those with large nuclear perimeters are shown in the lower left panel, lower middle panel, and lower right panel, respectively. Fig. S3. Frequency distribution of BALF lymphocyte nuclear radius ratios. The dark gray bars represent the frequency distribution of the BALF lymphocyte nuclear radius ratios in sarcoidosis patients, while the light gray bars represent those in the other ILD patients. The vertical dotted line represents the sarcoidosis diagnostic threshold of the nuclear radius ratio (1.346). Representative lymphocytes with small nuclear radius ratios, those near the threshold value, and those with large nuclear radius ratios are shown in the lower left panel, lower middle panel, and lower right panel, respectively. Fig. S4. Frequency distribution of BALF lymphocyte nuclear roundness. The dark gray bars represent the frequency distribution of BALF lymphocyte nuclear roundness in sarcoidosis patients, while the light gray bars represent those in the other ILD patients. The vertical dotted line represents the sarcoidosis diagnostic threshold for nuclear roundness (1.0057). Representative lymphocytes with small nuclear roundness, those near the threshold value, and those with large nuclear roundness are shown in the lower left panel, lower middle panel, and lower right panel, respectively. Fig. S5. The four morphological parameters of BALF lymphocyte nuclei. The figures are representing the definition of area, perimeter, radius ratio and roundness of lymphocyte nucleus