cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Phosphorylation of Protease-activated Receptor-2 Differentially Regulates Desensitization and Internalization
Creators: name: Trejo, Jo Ann
name: Ricks, Tiffany K.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2009

description: Protease-activated receptor 2 (PAR2) is a G protein-coupled receptor irreversibly activated by extracellular proteases. Activated PAR2 couples to multiple heterotrimeric G-protein subtypes including Gαq, Gαi, and Gα12/13. Most activated G protein-coupled receptors are rapidly desensitized and internalized following phosphorylation and β-arrestin binding. However, the role of phosphorylation in regulation of PAR2 signaling and trafficking is not known. To investigate the function of phosphorylation, we generated a PAR2 mutant in which all serines and threonines in the C-tail were converted to alanines and designated it PAR2 0P. In mammalian cells, the addition of agonist induced a rapid and robust increase in phosphorylation of wild-type PAR2 but not the 0P mutant, suggesting that the major sites of phosphorylation occur within the C-tail domain. Moreover, desensitization of PAR2 0P signaling was markedly impaired compared with the wild-type receptor. Wild-type phosphorylated PAR2 internalized through a canonical dynamin, clathrin- and β-arrestin-dependent pathway. Strikingly, PAR2 0P mutant internalization proceeded through a dynamin-dependent but clathrin- and β-arrestin-independent pathway in both a constitutive and agonist-dependent manner. Collectively, our studies show that PAR2 phosphorylation is essential for β-arrestin binding and uncoupling from heterotrimeric G-protein signaling and that the presence of serine and threonine residues in the PAR2 C-tail hinder constitutive internalization through a non-canonical pathway. Thus, our studies reveal a novel function for phosphorylation that differentially regulates PAR2 desensitization and endocytic trafficking.
Ribosomal S6 Kinase 2 Directly Phosphorylates the 5-Hydroxytryptamine 2A (5-HT 2A ) Serotonin Receptor, Thereby Modulating 5-HT 2A Signaling
Creators: name: Kiselar, Janna G.
name: Roth, Bryan L.
name: Kinter, Michael
name: Strachan, Ryan T.
name: Willard, Belinda
name: Sheffler, Douglas J.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2009

description: The 5-hydroxytryptamine 2A (5-HT2A) receptor is a member of the G protein-coupled receptor superfamily (GPCR) and plays a key role in transducing a variety of cellular signals elicited by 5-hydroxytryptamine in both peripheral and central tissues. Despite its broad physiological importance, our current understanding of 5-HT2A receptor regulation is incomplete. We recently reported the novel finding that the multifunctional ERK effector ribosomal S6 kinase 2 (RSK2) physically interacts with the 5-HT2A receptor third intracellular (i3) loop and modulates receptor signaling (Sheffler, D. J., Kroeze, W. K., Garcia, B. G., Deutch, A. Y., Hufeisen, S. J., Leahy, P., Bruning, J. C., and Roth, B. L. (2006) Proc. Natl. Acad. Sci. U. S. A. 103, 4717–4722). We report here that RSK2 directly phosphorylates the 5-HT2A receptor i3 loop at the conserved residue Ser-314, thereby modulating 5-HT2A receptor signaling. Furthermore, these studies led to the discovery that RSK2 is required for epidermal growth factor-mediated heterologous desensitization of the 5-HT2A receptor. We arrived at these conclusions via multiple lines of evidence, including in vitro kinase experiments, tandem mass spectrometry, and site-directed mutagenesis. Our findings were further validated using phospho-specific Western blot analysis, metabolic labeling studies, and whole-cell signaling experiments. These results support a novel regulatory mechanism in which a downstream effector of the ERK/MAPK pathway directly interacts with, phosphorylates, and modulates signaling of the 5-HT2A serotonin receptor. To our knowledge, these findings are the first to demonstrate that a downstream member of the ERK/MAPK cascade phosphorylates a GPCR as well as mediates cross-talk between a growth factor and a GPCR.
(4026) Cleonice Ridgegway
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Symmetric Complementary Logic Inverter Using Integrated Black Phosphorus and MoS2 Transistors
Creators: name: Su, Yang
name: Kshirsagar, Chaitanya U.
name: Robbins, Matthew C.
name: Haratipour, Nazila
name: Koester, Steven J.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Mesoscale and Nanoscale Physics (cond-mat.mes-hall)
subject: FOS: Physical sciences

description: The operation of an integrated two-dimensional complementary metal-oxide-semiconductor inverter with well-matched input/output voltages is reported. The circuit combines a few-layer MoS2 n-MOSFET and a black phosphorus (BP) p-MOSFET fabricated using a common local backgate electrode with thin (20 nm) HfO2 gate dielectric. The constituent devices have linear threshold voltages of -0.8 V and +0.8 V and produce peak transconductances of 16 uS/um and 41 uS/um for the MoS2 n-MOSFET and BP p-MOSFET, respectively. The inverter shows a voltage gain of 3.5 at a supply voltage, VDD = 2.5 V, and has peak switching current of 108 uA and off-state current of 8.4 uA (2.4 uA) at VIN = 0 (VIN = 2.5 V). In addition, the inverter has voltage gain greater than unity for VDD > 0.5 V, has open butterfly curves for VDD > 1 V, and achieves static noise margin over 500 mV at VDD = 2.5 V. The voltage gain was found to be insensitive to temperature between 270 K and 340 K, and AC large and small-signal operation was demonstrated at frequencies up to 100 kHz. The demonstration of a complementary 2D inverter which operates in a symmetric voltage window suitable for driving a subsequent logic stage is a significant step forward in developing practical applications for devices based upon 2D materials.
Additional file 1 of Application of a Reactive Agility Training Program Using Light-Based Stimuli to Enhance the Physical and Cognitive Performance of Car Racing Drivers: A Randomized Controlled Trial
Creators: name: Horváth, Dávid
name: Négyesi, János
name: Győri, Tamás
name: Szűcs, Botond
name: Tóth, Péter János
name: Matics, Zsolt
name: Ökrös, Csaba
name: Sáfár, Sándor
name: Szabó, Nikolett
name: Takács, Beáta
name: Kathy, Róbert
name: Tóth, Klára
name: Ferguson, David P.
name: Nagatomi, Ryoichi
name: Rácz, Levente
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Neuroscience
subject: Physiology
subject: FOS: Biological sciences
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: 111714 Mental Health
subject: FOS: Health sciences

description: Additional file 1. CONSORT Checklist.
title: Use of arterial spin-labeling characteristics as a predictor of glioma grade
Creators: name: Wood, Verity
Publisher: name: The Royal Australian and New Zealand College of Radiologists
Year: 2017
Subjects: subject: Neuroradiology brain
subject: Vascular
subject: MR physics
subject: MR
subject: Imaging sequences
subject: Neoplasia
subject: Image registration
subject: Pathology

description: Purpose Methods and materials Results Conclusion Personal information References
description: Purpose: Arterial spin labeling (ASL) is a relatively new but increasingly available technique in magnetic resonance imaging (MRI). It provides a measure of cerebral blood flow (CBF) by electromagnetically labelling inflowing arterial blood water to allow...
Stress-dependent Daxx-CHIP Interaction Suppresses the p53 Apoptotic Program
Creators: name: Charles, Peter C.
name: Portbury, Andrea
name: Cyr, Douglas M.
name: Qian, Shu Bing
name: Hilliard, Eleanor G.
name: McDonough, Holly
name: Min, Jin Na
name: Patterson, Cam
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2009

description: Our previous studies have implicated CHIP (carboxyl terminus of Hsp70-interacting protein) as a co-chaperone/ubiquitin ligase whose activities yield protection against stress-induced apoptotic events. In this report, we demonstrate a stress-dependent interaction between CHIP and Daxx (death domain-associated protein). This interaction interferes with the stress-dependent association of HIPK2 with Daxx, blocking phosphorylation of serine 46 in p53 and inhibiting the p53-dependent apoptotic program. Microarray analysis confirmed suppression of the p53-dependent transcriptional portrait in CHIP+/+ but not in CHIP−/− heat shocked mouse embryonic fibroblasts. The interaction between CHIP and Daxx results in ubiquitination of Daxx, which is then partitioned to an insoluble compartment of the cell. In vitro ubiquitination of Daxx by CHIP revealed that ubiquitin chain formation utilizes non-canonical lysine linkages associated with resistance to proteasomal degradation. The ubiquitination of Daxx by CHIP utilizes lysines 630 and 631 and competes with the sumoylation machinery of the cell at these residues. These studies implicate CHIP as a stress-dependent regulator of Daxx that counters the pro-apoptotic influence of Daxx in the cell. By abrogating p53-dependent apoptotic pathways and by ubiquitination competitive with Daxx sumoylation, CHIP integrates the proteotoxic stress response of the cell with cell cycle pathways that influence cell survival.
The complex chemistry of outflow cavity walls exposed: the case of low-mass protostars
Creators: name: Drozdovskaya, Maria N.
name: Walsh, Catherine
name: Visser, Ruud
name: Harsono, Daniel
name: van Dishoeck, Ewine F.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Solar and Stellar Astrophysics (astro-ph.SR)
subject: FOS: Physical sciences

description: Complex organic molecules are ubiquitous companions of young low-mass protostars. Recent observations suggest that their emission stems, not only from the traditional hot corino, but also from offset positions. In this work, 2D physicochemical modelling of an envelope-cavity system is carried out. Wavelength-dependent radiative transfer calculations are performed and a comprehensive gas-grain chemical network is used to simulate the physical and chemical structure. The morphology of the system delineates three distinct regions: the cavity wall layer with time-dependent and species-variant enhancements; a torus rich in complex organic ices, but not reflected in gas-phase abundances; and the remaining outer envelope abundant in simpler solid and gaseous molecules. Strongly irradiated regions, such as the cavity wall layer, are subject to frequent photodissociation in the solid phase. Subsequent recombination of the photoproducts leads to frequent reactive desorption, causing gas-phase enhancements of several orders of magnitude. This mechanism remains to be quantified with laboratory experiments. Direct photodesorption is found to be relatively inefficient. If radicals are not produced directly in the icy mantle, the formation of complex organics is impeded. For efficiency, a sufficient number of FUV photons needs to penetrate the envelope; and elevated cool dust temperatures need to enable grain-surface radical mobility. As a result, a high stellar luminosity and a sufficiently wide cavity favor chemical complexity. Furthermore within this paradigm, complex organics are demonstrated to have unique lifetimes and be grouped into early (formaldehyde, ketene, methanol, formic acid, methyl formate, acetic acid, glycolaldehyde) and late (acetaldehyde, dimethyl ether, ethanol) species.
description: Accepted for publication in MNRAS; 24 pages, 20 figures, 3 tables
title: The impact of antibiotic pre-treatment on diagnostic yield of CT-guided biopsy for spondylodiscitis: A multicentre retrospective study and meta-analysis.
Creators: name: Tarr, Gregory
Publisher: name: The Royal Australian and New Zealand College of Radiologists
Year: 2017
Subjects: subject: Musculoskeletal spine
subject: MR
subject: CT
subject: Biopsy
subject: Acute

description: Purpose Methods and materials Results Conclusion Personal information References
description: Purpose: Optimal treatment of spondylodiscitis requires accurate microbiological information, and radiology-guided percutaneous biopsy plays an important role in directly sampling lesion material. However, patients undergoing percutaneous biopsy have often...
Organisation spatiale des peuplements macrozoobenthiques subtidaux d'un estuaire de la côte atlantique marocaine : l'estuaire du Bou Regreg
Creators: name: CHERKAOUI, Essediya
name: BAYED, Abdellatif
name: HILY, Christian
Publisher: name: Station Biologique de Roscoff (SBR)
Year: 2003

description: Spatial organization of the macrobenthos in the Bou Regreg estuary, Moroccan Atlantic coast. On the Moroccan
 Atlantic coast, the Bou Regreg estuary has been disrupted by many human activities since more than 30 years, in relation
 with the extension of Rabat and Salé cities on its strands and especially the installation upstream of a dam in 1974. The aims
 of this work, realized from March 1998 to January 1999, were to establish a first ecological assessment of the subtidal
 ecosystem of the estuary after the achievement of the dam, before the establishment of a large development of the area. The
 results of hydrological analyses showed an upstream shifting of salinity in comparison with the previous situation.
 Multivariate analyses showed three communities successively distributed from the sea to the upper part of the estuary. The
 hydrological factors expressed by the distance from the mouth of the estuary seem to be the main factors that controlled the
 three macrozoobenthic communities, from downstream to upstream respectively: (1) the Haustorius arenarius community,
 composed of two assemblages dominated respectively by Gastrosaccus spinifer and Solen marginatus; (2) the
 Cerastoderma edule community, characterized by a high species richness, dominated by tolerant species with opportunistic
 ones, indicating a strong organic matter impact; (3) the Scrobicularia plana community that characterizes estuarine
 environmental conditions. Biotic indices values, using ecological groups of macrofauna species, indicated that this estuary
 is globally unbalanced, excepted at the mouth part. A comparison with the situation before the construction of the dam in
 Reçu le 23 janvier 2003 ; accepté après révision le 15 octobre 2003
 Received 23 January 2003; accepted in revised form 15 October 2003
Introduction
 Rompant la linéarité des côtes atlantiques marocaines,
 l'oued Bou Regreg et son bassin versant constituent un
 milieu naturel exceptionnel ayant favorisé l'implantation de
 populations humaines et le développeme
Resource costs for fault-tolerant linear optical quantum computing
Creators: name: Li, Ying
name: Humphreys, Peter C.
name: Mendoza, Gabriel J.
name: Benjamin, Simon C.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Quantum Physics (quant-ph)
subject: FOS: Physical sciences

description: Linear optical quantum computing (LOQC) seems attractively simple: information is borne entirely by light and processed by components such as beam splitters, phase shifters and detectors. However this very simplicity leads to limitations, such as the lack of deterministic entangling operations, which are compensated for by using substantial hardware overheads. Here we quantify the resource costs for full scale LOQC by proposing a specific protocol based on the surface code. With the caveat that our protocol can be further optimised, we report that the required number of physical components is at least five orders of magnitude greater than in comparable matter-based systems. Moreover the resource requirements grow higher if the per-component photon loss rate is worse than one in a thousand, or the per-component noise rate is worse than $10^{-5}$. We identify the performance of switches in the network as the single most influential factor influencing resource scaling.
title: Retrospective audit of new diffusion weighted imaging change following cerebral angiography at a single institution over a three year period.
Creators: name: Macdonald, Mark
Publisher: name: The Royal Australian and New Zealand College of Radiologists
Year: 2017
Subjects: subject: Neuroradiology brain
subject: MR-Diffusion/Perfusion
subject: Complications
subject: Ischaemia / Infarction

description: Purpose Methods and materials Results Conclusion Personal information References
description: Purpose: Microembolic phenomena detected on diffusion weighted imaging (DWI) are increasingly recognised to be common following neuroendovascular and endovascular cardiac procedures. We sought to evaluate the incidence of DWI focifollowing diagnostic and...
SÉLECTION DES GRAINS DU TUBE CHEZ LES AMPHICTENIDAE (POLYCHÈTES SÉDENTAIRES)
Creators: name: VOVELLE, Jean
Publisher: name: Station Biologique de Roscoff (SBR)
Year: 1971

description: The tubes of four species belonging to the Amphictenid Polychaetes have been collected from the slimy or conchitic bottoms of the Gullmarfjord and the Lysekil Archipelago for granulometric studies of their possible dépendance on thesediment.
Every species presents a caracteristic maximum of the dimensions of grains, which is the same in the different collecting places and comparable with values established for previously studied biotops in Brittany or Normandy: as it is always discordant with the granulometric graph of the surrounding sediment,it denotes an active selection which each species in a peculiar spot expresses by its own original interpretation. These granulometric data depend on the growth of the animal and are related to the building-organs complex and its specific behaviour: so can be explained the difference between the tubes of Pectinaria and Petta.
title: An audit of CT guided lung biopsies for diagnostic adequacy and complication rates in a teaching hospital
Creators: name: Brennan, Tommy
Publisher: name: The Royal Australian and New Zealand College of Radiologists
Year: 2017
Subjects: subject: Plain radiographic studies
subject: CT
subject: Thorax
subject: Interventional non-vascular
subject: Lung
subject: Percutaneous
subject: Biopsy
subject: Audit and standards
subject: Safety
subject: Cancer
subject: Pathology
subject: Outcomes

description: Purpose Methods and materials Results Conclusion Personal information References
description: Purpose: Computed tomography (CT) guided lung biopsies are utilised in the pathological diagnosis of masses identified in patient imaging and have a significant influence on patient management. The aims of this audit are: 1. To assess the Logan Hospital...
An Analysis of B-L=-2 Operators from Matter-Higgs Interactions in a Class of Supersymmetric SO(10) Models
Creators: name: Nath, Pran
name: Syed, Raza M.
Publisher: name: arXiv
Year: 2015
Subjects: subject: High Energy Physics - Phenomenology (hep-ph)
subject: High Energy Physics - Theory (hep-th)
subject: FOS: Physical sciences

description: Recently interest in GUT baryogenesis has been resurrected due to the observation that B-violating dimension seven operators that arise in grand unified theories that also violate B-L produce baryon asymmetry that cannot be wiped out by sphaleron processes. While a general analysis of such higher dimensional operators from a bottom up approach exists in the literature, a full analysis of them derived from grand unification does not exist. In this work we present a complete analysis of B-L=-2 operators within a realistic SO(10) grand unification where the doublet-triplet splitting is automatic via a missing partner mechanism. Specifically we compute all allowed dimension five, dimension seven and dimension nine operators arising from matter-Higgs interactions. The relative strength of all the allowed B-L=-2 operators is given. Such interactions are useful in the study of neutrino masses, baryogenesis, proton decay and $n-\bar n$ oscillations within a common realistic grand unification framework.
description: 30 pages. Accepted for publication in PRD
title: Case Series: Traumatic Testicular Injury on Polytrauma CT.
Creators: name: Lu, Shisheng
Publisher: name: The Royal Australian and New Zealand College of Radiologists
Year: 2017
Subjects: subject: Genital / Reproductive system male
subject: Emergency
subject: CT
subject: Diagnostic procedure
subject: Education
subject: Trauma

description: Learning objectives Background Imaging findings OR Procedure details Conclusion Personal information References
description: Learning objectives: This poster pays particular focus on CT manifestations of testicular trauma. This poster also aims to raise awareness of anuncommon entity called testicular dislocation, which is less well known in the radiology...
(4023) John Stuart Wallace
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
title: Das ärztliche Ethos - zwischen Bedeutung, Etablierungsproblematik und Erneuerung
Creators: name: Hajek-Prouza, Karin
Year: 2022

description: Diese Arbeit widmet sich der Frage, ob das ärztliche Ethos neu zu denken ist. Dabei wird der Versuch seiner Verortung zwischen historischer Bedeutung und gegenwärtigen Gefahren unternommen. Dies dient der Klärung des Vorhandenseins von basalen Instabilitäten und einer multikausalen Etablierungsproblematik, die eine erneuerte Herangehensweise an das ärztliche Ethos nötig werden lässt. Daher präsentiere ich das Neudenken des ärztlichen Ethos in einer strukturellen Gleichsetzung mit der Verantwortung und zugleich als fundamentale Vernetzungsaufgabe des Arztes, die in einer Über- und Annahme und nicht in einem bloßen Haben von Verantwortung besteht. Für den praktischen Kontext bedeutet dies, dass eine Evaluierung der Zugangsbeschränkungen zum Human- und Zahnmedizinstudium dringend erforderlich ist.
description: My thesis is dedicated to the question whether medical ethos needs to be rethought or not. An attempt is made to locate the concept of a medical ethos between historical significance and modern dangers. This serves to clarify the existence of basal instabilities and a multicausal establishment problem, which requires a renewed approach to medical ethos. Thus, I present the rethinking of the medical ethos in a structural equation with responsibility and at the same time as a fundamental networking task of the doctor, which consists in accepting and not in simply having responsibility. For the practical context, this means that an evaluation of the access restrictions to medical and dental studies is urgently recommended.
Additional file 2 of Application of a Reactive Agility Training Program Using Light-Based Stimuli to Enhance the Physical and Cognitive Performance of Car Racing Drivers: A Randomized Controlled Trial
Creators: name: Horváth, Dávid
name: Négyesi, János
name: Győri, Tamás
name: Szűcs, Botond
name: Tóth, Péter János
name: Matics, Zsolt
name: Ökrös, Csaba
name: Sáfár, Sándor
name: Szabó, Nikolett
name: Takács, Beáta
name: Kathy, Róbert
name: Tóth, Klára
name: Ferguson, David P.
name: Nagatomi, Ryoichi
name: Rácz, Levente
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Neuroscience
subject: Physiology
subject: FOS: Biological sciences
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: 111714 Mental Health
subject: FOS: Health sciences

description: Additional file 2. Placement of the 8 LED lamps during the reactive agility training program.
Structural and Biophysical Characterization of the Proteins Interacting with the Herpes Simplex Virus 1 Origin of Replication
Creators: name: Svergun, Dmitri
name: Griffith, Jack D.
name: Konarev, Peter
name: Kalkhof, Stefan
name: Sinz, Andrea
name: Ticker, Paul A.
name: Malchov, Alexander M.
name: Cary, Peter D.
name: Fullerton, Stephen W.
name: Mumtsidu, Eleni
name: Kneale, Geoff G.
name: Manolaridis, Ioannis
name: McGeehan, John E.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2009

description: The C terminus of the herpes simplex virus type 1 origin-binding protein, UL9ct, interacts directly with the viral single-stranded DNA-binding protein ICP8. We show that a 60-amino acid C-terminal deletion mutant of ICP8 (ICP8ΔC) also binds very strongly to UL9ct. Using small angle x-ray scattering, the low resolution solution structures of UL9ct alone, in complex with ICP8ΔC, and in complex with a 15-mer double-stranded DNA containing Box I of the origin of replication are described. Size exclusion chromatography, analytical ultracentrifugation, and electrophoretic mobility shift assays, backed up by isothermal titration calorimetry measurements, are used to show that the stoichiometry of the UL9ct-dsDNA15-mer complex is 2:1 at micromolar protein concentrations. The reaction occurs in two steps with initial binding of UL9ct to DNA (Kd ∼ 6 nm) followed by a second binding event (Kd ∼ 0.8 nm). It is also shown that the stoichiometry of the ternary UL9ct-ICP8ΔC-dsDNA15-mer complex is 2:1:1, at the concentrations used in the different assays. Electron microscopy indicates that the complex assembled on the extended origin, oriS, rather than Box I alone, is much larger. The results are consistent with a simple model whereby a conformational switch of the UL9 DNA-binding domain upon binding to Box I allows the recruitment of a UL9-ICP8 complex by interaction between the UL9 DNA-binding domains.
(4024) Mary J[amieson] Wallace
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Reproductive biology of the silver scabbard fish Lepidopus caudatus (Euphrasen, 1788) in the East coast of Tunisia
Creators: name: DHAKER, Troudi
name: RIMEL, Benmessaoud
name: WAFA, Hajlaoui
name: MISSAOUI, Hechmi
Publisher: name: Station Biologique de Roscoff (SBR)
Year: 2016

description: The present work constitutes a contribution to study the different aspects of sexual cycle of Lepidopus caudatus. The sex ratio showed a significant difference. It varied with the size and females were significantly more likely in larger sizes. The size at first maturity was reached at 94.6 to 100.3 cm respectively in males and females. Breeding season was between September and December, with maximum spawning period in September. The K condition factor was high in the summer period before the start of reproduction. This implied an accumulation of reserves before the breeding season. The mean absolute fecundity was 260,518 (± 45,250) by act of laying eggs and mature female with a mean total length of 132.4 (± 30.7) cm. The relative fecundity is 133 (± 54) oocytes.g-1 of mature female. The absolute fecundity increases with total length and total weight of individuals.
Mario Faust-Scalisi - Intersectionality and Activism - Chilean Fanzines (paper)
Creators: name: Faust-Scalisi, Mario
Publisher: name: University of the Arts London
Year: 2020
Subjects: subject: 199999 Studies in the Creative Arts and Writing not elsewhere classified
subject: FOS: Arts (arts, history of arts, performing arts, music)

description: Chile witnesses multiple forms of intersectional discriminations as gender or racist on a daily basis. We know this latest since the performance ‘Un violador en tu camino’ by Las-Tesis went global. In Chile some fight for social justice on the street, other by raising awareness with comics, more specific with self-printed non-sexist activist-fanzines. For this stands the self-declared feminist comic collective Las Tetas, and especially the linked artist Katherine Supnem. In these fanzines dealing with personal intersectional discrimination experiences is combined with fighting for social justice and minority rights in Chile and beyond by raising awareness of discriminations and injustice happening – here the idea of “personalized politics” by fanzines can be witnessed empirically (see Bobel 2010, p. 115ff.). By linking personal intersectional challenges with society problems these fanzines get credibility and at the same time reach. But activism doesn’t stop with drawing and writing comics, with the question of getting a voice as discussed by Roth (2004). In their distribution or presentation activist approaches are followed, too. At the same time comic authors like Supnem come together with others to not only fight injustice with pen or paper, but on the streets, in workshops or elsewhere . Drawing comics empowers Supnem personally, and her fanzines empower others to fight diverse forms of intersectional discrimination in multiple ways (see for the question of empowerment and multiple forms of expression e.g. Dale 2012, S. 149ff.).
Recruit survival of Cymodocea nodosa along a depth gradient
Creators: name: PAULO, Diogo
name: MANENT, Pablo
name: BARRIO, Juan M.
name: SERRÃO, Ester Álvares
name: ALBERTO, Filipe
Publisher: name: Station Biologique de Roscoff (SBR)
Year: 2016

description: Clonal plants can reproduce sexually and clonally, expressing different proportions of these different life histories in distinctive environments. In seagrass meadows, light attenuation hinders marine plant colonization in deeper areas of its vertical distribution. Therefore it is expected that the fluorescence maximum quantum yield of seedlings will be higher at deep limits of the meadow relative to shallower depths. We hypothesized that seagrass seedlings experience higher mortality in deeper areas of the meadows than at shallower levels. Our objective was to test survival of seagrass seedlings along a depth gradient in meadows of Cymodocea nodosa in the Canary Islands. Seedlings germinated in laboratory conditions, were transplanted to a natural meadow at three water depths: shallow (5 m), medium (8 m) and deep (12 m). Transplanted seedlings were monitored by measuring maximum quantum yield, leaf length, number of leaves per shoot and survival. Our data suggest that light does not hinder survival of seedlings along the depth gradient of the meadow. Before establishing clonal growth, seedlings in deeper areas had higher survival rates than those in shallow areas. Even though seeds are present at shallow depths and sexual allocation is high, sexual contribution can be lower than expected due to seedling loss.
Structural Determinants of G-protein α Subunit Selectivity by Regulator of G-protein Signaling 2 (RGS2)
Creators: name: Urban, Daniel J.
name: Temple, Brenda R.S.
name: Knapp, Stefan
name: Roos, Annette K.
name: Willard, Francis S.
name: Roth, Bryan L.
name: Kimple, Adam J.
name: Siderovski, David P.
name: Soundararajan, Meera
name: Hutsell, Stephanie Q.
name: Setola, Vincent
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2009

description: “Regulator of G-protein signaling” (RGS) proteins facilitate the termination of G protein-coupled receptor (GPCR) signaling via their ability to increase the intrinsic GTP hydrolysis rate of Gα subunits (known as GTPase-accelerating protein or “GAP” activity). RGS2 is unique in its in vitro potency and selectivity as a GAP for Gαq subunits. As many vasoconstrictive hormones signal via Gq heterotrimer-coupled receptors, it is perhaps not surprising that RGS2-deficient mice exhibit constitutive hypertension. However, to date the particular structural features within RGS2 determining its selectivity for Gαq over Gαi/o substrates have not been completely characterized. Here, we examine a trio of point mutations to RGS2 that elicits Gαi-directed binding and GAP activities without perturbing its association with Gαq. Using x-ray crystallography, we determined a model of the triple mutant RGS2 in complex with a transition state mimetic form of Gαi at 2.8-Å resolution. Structural comparison with unliganded, wild type RGS2 and of other RGS domain/Gα complexes highlighted the roles of these residues in wild type RGS2 that weaken Gαi subunit association. Moreover, these three amino acids are seen to be evolutionarily conserved among organisms with modern cardiovascular systems, suggesting that RGS2 arose from the R4-subfamily of RGS proteins to have specialized activity as a potent and selective Gαq GAP that modulates cardiovascular function.
Age, growth and reproduction of the striped sea bream Lithognathus mormyrus (Linnaeus, 1758) in the gulf of Gabes (Southeastern Tunisia, Central Mediterranean)
Creators: name: BEN ABDALLAH-BEN HADJ HAMIDA, Olfa
name: BEN HADJ HAMIDA, Nader
name: CHAOUCH, Houda
name: JARBOUI, Othman
name: MISSAOUI, Hechmi
Publisher: name: Station Biologique de Roscoff (SBR)
Year: 2016

description: The striped sea bream Lithognathus mormyrus (Linnaeus, 1758) is a commercially valuable sparid fish frequently caught and landed off the Gulf of Gabes (southern coasts of Tunisia). Age, growth and reproductive aspects of the southeastern Tunisian population were estimated by analyzing 1221 specimens ranging in total length (TL) from 9.7 to 24.1 cm. Ages were estimated by counting growth bands on otoliths and verified by marginal increment analysis, with specimens ranging in age from 1 to 8 years. The von Bertalanffy growth function was selected as the most adequate model to fit this species' growth, with the estimated parameters being L∞ = 31.22 cm TL, k = 0.112 year-1 and t0 = -2.307 years. Gonads were analyzed macroscopically over a one-year period and the spawning season found to occur from September to November. The length at first maturity for males occurs at 13.99 cm TL and 3.4 years while females mature at slightly larger size (14.32 cm TL) and earlier age (3.1 years). The results presented in this study purpose to fill the gaps in knowledge concerning aspects of population dynamics, namely age and growth and reproduction of striped seabream in the Gulf of Gabes.
Structure of Long Telomeric RNA Transcripts: THE G-RICH RNA FORMS A COMPACT REPEATING STRUCTURE CONTAINING G-QUARTETS
Creators: name: Griffith, Jack D.
name: Randall, Adrian
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2009

description: Recent studies have identified RNA transcripts arising from mammalian telomeres with the transcript of the C-rich strand (r(5′-UUAGGG-3′)n) being much more abundant than transcripts of the G-rich strand. Here we used transmission electron microscopy, CD, and nuclease digestion to investigate the structure of ∼640-nucleotide (nt) RNA transcripts of the C-rich and G-rich strands of mammalian telomeric DNA. The CD spectrum of the C-rich RNA in low salt (10 mm KCl) or high salt (100 mm KCl) was typical of mixed sequence RNA, whereas the CD spectrum for the G-rich RNA differed with changes characteristic of parallel G-quadruplexes at the higher salt concentration. Electron microscopy visualization of the C-rich RNA revealed relatively extended unstructured molecules 59.7 ± 17.8 nm in length and with a width consistent with single-stranded RNA following metal coating. In contrast, the G-rich RNA was observed as round particles and short, thick rods with the rods being most prevalent in high salt conditions and absent in low salt. The rods were 22.7 ± 4.8 nm in length and 7.6 nm in width. Digestion of the G-rich RNA with T1 RNA nuclease revealed a ladder of bands whose sizes were integral multiples of 24 nt plus a 4-nt overhang. These observations suggest a model in which G-rich telomeric RNA folds into chains of particles each consisting of four (UUAGGG) repeats stabilized by parallel G-quartets and joined by UUA linkers. These chains further condense to form short rods and round particles.
Confirmed occurrence in the Mediterranean Sea of milk shark Rhizoprionodon acutus (Chondrichthyes: Carcharhinidae) and first record off the Tunisian coast
Creators: name: BEN AMOR, Mohamed Mourad
name: DIATTA, Youssouph
name: DIOP, Mansor
name: BEN SALEM, Mohamed
name: CAPAPÉ, Christian
Publisher: name: Station Biologique de Roscoff (SBR)
Year: 2016

description: The authors present in this paper the second Mediterranean record of milk shark Rhizoprionodon acutus (Rüppell, 1837) which is also the first record from the Tunisian coast. The specimen was captured off Zarzis in southern Tunisia, it is described together with a specimen caught off the Senegalese coast, used as reference for identification. The occurrence of the species in the area and the Mediterranean Sea is discussed and commented. It appears that the origin of the Mediterranean specimens of R. acutus remains still questionnable.
Synaptotagmin 2 Couples Mucin Granule Exocytosis to Ca 2+ Signaling from Endoplasmic Reticulum
Creators: name: Davis, C. Williams
name: Tuvim, Michael J.
name: Chua, Michael
name: Mohler, Peter J.
name: Mospan, Andrea Rossi
name: Ammar-Aouchiche, Zoulikha
name: Burns, Kimberlie A.
name: Melicoff, Ernestina
name: Dickey, Burton F.
name: Adachi, Roberto
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2009

description: Synaptotagmin 2 (Syt2) functions as a low affinity, fast exocytic Ca2+ sensor in neurons, where it is activated by Ca2+ influx through voltage-gated channels. Targeted insertion of lacZ into the mouse syt2 locus reveals expression in mucin-secreting goblet cells of the airways. In these cells, rapid Ca2+ entry from the extracellular medium does not contribute significantly to stimulated secretion (Davis, C. W., and Dickey, B. F. (2008) Annu. Rev. Physiol. 70, 487–512). Nonetheless, Syt2–/– mice show a severe defect in acute agonist-stimulated airway mucin secretion, and Syt2+/– mice show a partial defect. In contrast to Munc13-2–/– mice (Zhu, Y., Ehre, C., Abdullah, L. H., Sheehan, J. K., Roy, M., Evans, C. M., Dickey, B. F., and Davis, C. W. (2008) J. Physiol. (Lond.) 586, 1977–1992), Syt2–/– mice show no spontaneous mucin accumulation, consistent with the inhibitory action of Syt2 at resting cytoplasmic Ca2+ in neurons. In human airway goblet cells, inositol trisphosphate receptors are found in rough endoplasmic reticulum that closely invests apical mucin granules, consistent with the known dependence of exocytic Ca2+ signaling on intracellular stores in these cells. Hence, Syt2 can serve as an exocytic sensor for diverse Ca2+ signaling systems, and its levels are limiting for stimulated secretory function in airway goblet cells.
title: The Introduction of a Multiuse Contrast Delivery System Results in Significant Savings
Creators: name: Thompson, Nadine
Publisher: name: The Royal Australian and New Zealand College of Radiologists
Year: 2017
Subjects: subject: Contrast agents
subject: Management
subject: CT
subject: Contrast agent-intravenous
subject: Economics

description: Purpose Methods and materials Results Conclusion Personal information References
description: Purpose: The cost of providing Radiology services is increasing and Medicare Schedule Fees are not increasing to match this rate Efficiencies and cost reduction strategiescan directly impact a radiology practice's profitability A large proportion of CT scans...
von Kàrmàn--Howarth and Corrsin equations closure based on Lagrangian description of the fluid motion
Creators: name: de Divitiis, Nicola
Publisher: name: arXiv
Year: 2015
Subjects: subject: Fluid Dynamics (physics.flu-dyn)
subject: Atmospheric and Oceanic Physics (physics.ao-ph)
subject: Classical Physics (physics.class-ph)
subject: FOS: Physical sciences

description: A new approach to obtain the closure formulas for the von Kármán--Howarth and Corrsin equations is presented, which is based on the Lagrangian representation of the fluid motion, and on the Liouville theorem associated to the kinematics of a pair of fluid particles. This kinematics, characterized by the finite--scale separation vector, is assumed to be statistically independent from the velocity field. Such assumption is justified by the hypothesis of fully developed turbulence and by the property that this vector varies much more rapidly than the velocity field. This formulation leads to the closure formulas of von Kármán--Howarth and Corrsin equations in terms of longitudinal velocity and temperature correlations following a demonstration completely different with respect to the previous works. Some of the properties and the limitations of the closed equations are discussed. In particular, the times of evolution of the developed kinetic energy and temperature spectra are shown to be finite quantities which depend on the initial conditions.
description: 32 pages, 2 figures, Annals of Physics, 2016
The Accessory SecA2 System of Mycobacteria Requires ATP Binding and the Canonical SecA1
Creators: name: Rigel, Nathan W.
name: McCann, Jessica R.
name: McDonough, Justin A.
name: Kurtz, Sherry
name: Gibbons, Henry S.
name: Braunstein, Miriam
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2009

description: In bacteria, the majority of exported proteins are transported by the general Sec pathway from their site of synthesis in the cytoplasm across the cytoplasmic membrane. The essential SecA ATPase powers this Sec-mediated export. Mycobacteria possess two nonredundant SecA homologs: SecA1 and SecA2. In pathogenic Mycobacterium tuberculosis and the nonpathogenic model mycobacterium Mycobacterium smegmatis, SecA1 is essential for protein export and is the “housekeeping” SecA, whereas SecA2 is an accessory SecA that exports a specific subset of proteins. In M. tuberculosis the accessory SecA2 pathway plays a role in virulence. In this study, we uncovered basic properties of the mycobacterial SecA2 protein and its pathway for exporting select proteins. By constructing secA2 mutant alleles that encode proteins defective in ATP binding, we showed that ATP binding is required for SecA2 function. SecA2 mutant proteins unable to bind ATP were nonfunctional and dominant negative. By evaluating the subcellular distribution of each SecA, SecA1 was shown to be equally divided between cytosolic and cell envelope fractions, whereas SecA2 was predominantly localized to the cytosol. Finally, we showed that the canonical SecA1 has a role in the process of SecA2-dependent export. The accessory SecA2 export system is important to the physiology and virulence of mycobacteria. These studies help establish the mechanism of this new type of specialized protein export pathway.
The Intrinsic Reactivity of ATP and the Catalytic Proficiencies of Kinases Acting on Glucose, N -Acetylgalactosamine, and Homoserine: A THERMODYNAMIC ANALYSIS
Creators: name: Wolfenden, Richard
name: Stockbridge, Randy B.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2009

description: To evaluate the rate enhancements produced by representative kinases and their thermodynamic basis, rate constants were determined as a function of changing temperature for 1) the spontaneous methanolysis of ATP and 2) reactions catalyzed by kinases to which different mechanisms of action have been ascribed. For each of these enzymes, the minor effects of changing viscosity indicate that kcat/Km is governed by the central chemical events in the enzyme-substrate complex rather than by enzyme-substrate encounter. Individual Arrhenius plots, obtained at intervals between pH 4.8 and 11.0, yielded ΔH‡ and TΔS‡ for the nonenzymatic methanolysis of ATP2−, ATP3−, and ATP4− in the absence of Mg2+. The addition of Mg2+ led to partly compensating changes in ΔH‡ and TΔS‡, accelerating the nonenzymatic methanolysis of ATP 11-fold at pH 7 and 25 °C. The rate enhancements produced by yeast hexokinase, homoserine kinase, and N-acetylgalactosamine kinase (obtained by comparison of their kcat/Km values in the presence of saturating phosphoryl acceptor with the second order rate constant for methanolysis of MgATP) ranged between 1012- and 1014-fold. Their nominal affinities for the altered substrates in the transition state were 2.1 × 10−16m for N-acetylgalactosamine kinase, 7.4 × 10−17m for homoserine kinase, and 6.4 × 10−18m for hexokinase. Compared with nonenzymatic phosphoryl transfer, all three kinases were found to produce major reductions in the entropy of activation, in accord with the likelihood that substrate juxtaposition and desolvation play prominent roles in their catalytic action.
title: Quality of Fat Saturation in triple echo T2 Dixon Versus Traditional STIR and T2 Fat Saturated Sequences
Creators: name: Zhou, Jingyu
Publisher: name: The Royal Australian and New Zealand College of Radiologists
Year: 2017
Subjects: subject: Neuroradiology spine
subject: Musculoskeletal spine
subject: MR
subject: Comparative studies
subject: Artifacts

description: Purpose Methods and materials Results Conclusion Personal information References
description: Purpose: To evaluate the quality of fat suppression in T2-weighted triple-echo Dixon compared to conventional short-tau inversion recovery (STIR) and T2 weighted fast recovery with fat suppression (T2...
On the quantum discord of general X states
Creators: name: Yurischev, M. A.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Quantum Physics (quant-ph)
subject: Information Theory (cs.IT)
subject: FOS: Physical sciences
subject: FOS: Computer and information sciences

description: Quantum discord Q is a function of density matrix elements. The domain of such a function in the case of two-qubit system with X density matrix may consist of three subdomains at most: two ones where the quantum discord is expressed in closed analytical forms (Q_{π/2} and Q_0) and an intermediate subdomain for which, to extract the quantum discord Q_θ, it is required to solve in general numerically a one-dimensional minimization problem to find the optimal measurement angle θ\in(0,π/2). Hence the quantum discord is given by a piecewise-analytic-numerical formula Q=\min{Q_{π/2}, Q_θ, Q_0}. Equations for determining the boundaries between these subdomains are obtained. The boundaries consist of bifurcation points. The Q_θ subdomains are discovered in the generalized Horodecki states, in the dynamical phase flip channel model, in the anisotropic spin systems at thermal equilibrium, in the heteronuclear dimers in an external magnetic field. We found that transitions between Q_θ subdomain and Q_{π/2} and Q_0 ones occur suddenly but continuously and smoothly, i.e., nonanalyticity is hidden and can be observed in higher derivatives of discord function.
description: 23 pages, 12 figures. arXiv admin note: text overlap with arXiv:1404.5735