title: Ð¤Ð¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð»Ð°Ð·ÐµÑÐ½Ð¾Ð³Ð¾ Ð¿ÑÑÐºÐ° Ð² Ð¾Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ðµ Ð¿ÑÐ¸Ð±Ð¾ÑÐ° ÐºÐ°Ð¿Ð¸Ð»Ð»ÑÑÐ½Ð¾Ð³Ð¾ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÐ·Ð° Ð¿ÑÐ¸ Ð¿Ð¾Ð¼Ð¾ÑÐ¸ Ð°Ð½Ð°Ð¼Ð¾ÑÑÐ½ÑÑ Ð¿ÑÐ¸Ð·Ð¼
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Laser beam formation in optical system of capillar electroforesis by anamorphic prisms
Creators: name: Ð¡ÐµÐ¼Ð¸ÑÐµÐ²Ð°, ÐÐºÐ°ÑÐµÑÐ¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð°Ð½Ð°Ð»Ð¸Ð·Ð°ÑÐ¾Ñ
ÑÐµÐºÐ²ÐµÐ½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
Ð°Ð½Ð°Ð¼Ð¾ÑÑÐ½ÑÐµ Ð¿ÑÐ¸Ð·Ð¼Ñ
Ð»Ð¸Ð½Ð·Ð° ÐÐ°ÑÑÐ»Ð»Ð°
Ð´ÐµÑÐµÐºÑÐ¾Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸
genetic analyzer
sequencing
anamorphic prisms
Powell lens
fluorescence detector

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description: Topic of graduate qualification work: "Laser beam formation in optical system of capillar electroforesis by anamorphic prisms". The object of the study is the optical system of genetic analyzer NANOFOR 05, which is a capillary electrophoresis device with laser-induced fluorescence detection. Genetic analyzer NANOFOR 05 is designed to determine the sequence of nucleotides in DNA (sequencing), as well as to determine the length and/or relative intensity of fluorescence of DNA fragments (fragment analysis). The aim of the work is to extend the laser beam in the optical system of the genetic analyzer from 1.6 mm to 3.6 mm to illuminate the object, which is a line of 24 capillaries filled with samples. Problems solved in the work: â Modeling the existing beam expansion scheme consisting of a pair of anamorphic prisms in the optical system of the genetic analyzer using the ZEMAX program and determining the maximum possible beam magnification for the given optical components (prisms). â Experimental construction of a scheme with a pair of anamorphic prisms, measurement of laser power and power loss values for different prism configuration relative to each other. Comparison of the results with the ZEMAX simulation results. â Simulation of an alternative beam expansion scheme using the Powell lens in ZEMAX. In this work, we performed computer modeling of optical schemes for laser beam expansion in the ZEMAX program and physical modeling of the scheme using a pair of anamorphic prisms, carried out experimental measurements of laser beam power at the output of the scheme with prisms, and drew conclusions about the possibilities of expanding the laser beam. The results will help increase the performance of the NANOFOR 05 genetic analyzer by increasing the number of samples measured simultaneously during the analysis, which entails reducing the time required to analyze a large number of samples and increasing the cost-effectiveness of the analyzer.