cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Estimation of lifespan distribution of motorcycles in Vietnam
Creators: name: Kurogi, Daiki
name: Kosai, Shoki
name: Lai, Thai P.
name: Nguyen, Duc Q.
name: Murakami, Genya
name: Yamasue, Eiji
Publisher: name: Technische Universität Berlin
Year: 2021
Subjects: subject: 500 Naturwissenschaften und Mathematik
subject: 600 Technik, Technologie
subject: 300 Sozialwissenschaften
subject: lifespan distribution
subject: population balance model
subject: Vietnam
subject: motorcycle

description: Demand for various products has rapidly increased in developing countries due to rapid urbanization, increase in population, and change in lifestyle, which potentially causes a significant amount of waste shortly. It is expected that, because of the rapid increase in wastes, the current capacity of facilities would not sufficiently meet the requirement of proper treatment and recycling, potentially leading to vital environmental pollution. In particular, Motorcycles are used as major transportation means in Vietnam. The average possession of motorcycles per capita is high, approximately 36% as of 2016, and it is expected to continuously increase due to economic growth. Although there are several informal recycling sectors such as “craft village”, hazardous substances are not adequately treated, causing air pollution and soil contamination in the surrounding environment. Therefore, it is of paramount importance to predict the number of wastes of motorcycles in Vietnam. This study assessed the lifespan distribution of motorcycles in Vietnam through questionnaire survey by using the Weibull distribution and predicted the number of motorcycle wastes and metals contained in obsolete motorcycles in Vietnam in 2019-2030. Considering the increase in the number of scrapped motorcycles and the demand for resources induced by motorcycles, the estimated value might assist policymakers in developing the strategy for efficient motorcycle recycling.
Auxin-binding Protein 1 Does Not Bind Auxin within the Endoplasmic Reticulum Despite This Being the Predominant Subcellular Location for This Hormone Receptor
Creators: name: Tian, H.
name: Jones, A. M.
name: Klambt, D.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: Auxin-binding protein 1 (ABP1) is a unique hormone receptor because it resides primarily in the lumen of the endoplasmic reticulum (ER); however, two lines of evidence presented here suggest that ABP1 does not bind auxin within the endoplasmic reticulum, despite its predominant location there. First, ABP1 cannot be photolabeled in intact cells that have accumulated the auxin and photolabeling reagent 5-[7-3H]azidoindole-3-acetic acid, indicating either that auxin is excluded from the ER and is not available for photolabeling to ABP1 or that binding conditions within the ER lumen are insufficient for photolabeling. Second, at the pH of the ER lumen, auxin binding to ABP1 is not detectable. The pH estimate of the ER lumen is based on an indirect assay, which indicates that the pH is closer to pH 7 than to the binding optimum of pH 5.5. These results indicate that ABP1 does not bind auxin within the ER and point to a site of action that is post-ER. The effect of auxin on its trafficking from the ER was tested in an animal expression system. ABP1 expressed at high levels in COS7 cells is efficiently retained in the ER lumen and is not secreted even in the presence of 190 microM indole-3-acetic acid, an auxin concentration that is 40 times above the Kd for indole-3-acetic acid binding to ABP1.
Biogenesis of ISP6, a Small Carboxyl-terminal Anchored Protein of the Receptor Complex of the Mitochondrial Outer Membrane
Creators: name: Cao, W.
name: Douglas, M. G.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: To study the biogenesis of ISP6, an outer membrane component of the mitochondrial protein translocation complex, two fusion proteins have been made by fusing ISP6 to either the carboxyl- or amino-terminal end of the mouse dihydrofolate reductase (DHFR). In vitro import experiments showed that when DHFR was placed at the carboxyl-terminal end of ISP6, the resulting fusion protein 6-DHFR inserted into mitochondrial membrane less efficiently than the other form of the fusion proteins. In vivo this fusion protein lost its ability to suppress the temperature-sensitive phenotype of an isp42 mutant, while the other fusion protein DHFR-6, which was found targeted correctly to mitochondria, suppressed the mutant as well as the wild-type ISP6. Further analysis showed that the binding and insertion of DHFR-6 to mitochondrial outer membrane was not affected by deletion of either of the two mitochondrial protein receptors or by the predigestion of mitochondrial surface proteins prior to import. Additional data indicated that ISP42, which closely associates with ISP6 in the translocation complex, does not likely play the role of a targeting partner for ISP6. In summary, these data suggest that ISP6 may target to mitochondria by sequences at its carboxyl terminus and that the import process of ISP6 is most likely distinct from that of most other mitochondrial precursors, which are recognized by protein receptors on mitochondrial surface.
Additional file 1 of Compensation for wind drift prevails for a shorebird on a long-distance, transoceanic flight
Creators: name: Linscott, Jennifer A.
name: Navedo, Juan G.
name: Clements, Sarah J.
name: Loghry, Jason P.
name: Ruiz, Jorge
name: Ballard, Bart M.
name: Weegman, Mitch D.
name: Senner, Nathan R.
Publisher: name: figshare
Year: 2022
Subjects: subject: Ecology
subject: FOS: Biological sciences

description: Additional file 1: Methods—Procedural information for defining the migratory corridor, details on tolerance value selection, and R code for behavioral analyses. Results—Table S1: Results of LMMs evaluating the effect of wind support on measured ground speeds. Table S2: Model estimates for angular difference between realized and preferred directions in godwit northward migration. Table S3: Results of LMMs for godwit forward and lateral movement as a result of wind support and crosswind, respectively. Fig. S1: Movement tracks of individuals followed repeatedly over three years. Fig. S2: Comparison of behavioral classifications using a single preferred direction versus a range of the preferred direction. Fig. S3: For movements classified as fully compensating, godwit divergence from the preferred direction at varying wind speeds.
Geodynamic controls on clastic-dominated base metal deposits
Creators: name: Glerum, Anne C.
name: Brune, Sascha
name: Magnall, Joseph M.
name: Weis, Philipp
name: Gleeson, Sarah A.
Publisher: name: Freie Universität Berlin
Year: 2024
Subjects: subject: metal deposits
subject: mineralizing processes
subject: geodynamic controls
subject: 500 Naturwissenschaften und Mathematik::550 Geowissenschaften, Geologie::550 Geowissenschaften

description: To meet the growing global demand for metal resources, new ore deposit discoveries are required. However, finding new high-grade deposits, particularly those not exposed at the Earth's surface, is very challenging. Therefore, understanding the geodynamic controls on the mineralizing processes can help identify new areas for exploration. Here we focus on clastic-dominated Zn–Pb deposits, the largest global resource of zinc and lead, which formed in sedimentary basins of extensional systems. Using numerical modelling of lithospheric extension coupled with surface erosion and sedimentation, we determine the geodynamic conditions required to generate the rare spatiotemporal window where potential metal source rocks, transport pathways, and host sequences are present. We show that the largest potential metal endowment can be expected in narrow asymmetric rifts, where the mineralization window spans about 1–3 Myr in the upper ∼ 4 km of the sedimentary infill close to shore. The narrow asymmetric rift type is characterized by rift migration, a process that successively generates hyper-extended crust through sequential faulting, resulting in one wide and one narrow conjugate margin. Rift migration also leads to (1) a sufficient life span of the migration-side border fault to accommodate a thick submarine package of sediments, including coarse (permeable) continental sediments that can act as source rock; (2) rising asthenosphere beneath the thinned lithosphere and crust, resulting in elevated temperatures in these overlying sediments that are favourable for leaching metals from the source rock; (3) the deposition of organic-rich sediments that form the host rock at shallower burial depths and lower temperatures; and (4) the generation of smaller faults that cut the major basin created by the border fault and provide additional pathways for focused fluid flow from source to host rock. Wide rifts with rift migration can have similarly favourable configurations, but these occur less frequently and less potential source rock is produced, thereby limiting potential metal endowment. In simulations of narrow symmetric rifts, the conditions to form ore deposits are rarely fulfilled. Based on these insights, exploration programmes should prioritize the narrow margins formed in asymmetric rift systems, in particular regions within several tens of kilometres from the paleo-shoreline, where we predict the highest-value deposits to have formed.
All-Optical Generation of Surface Plasmons in Graphene
Creators: name: Constant, Thomas J.
name: Hornett, Samuel M.
name: Chang, Darrick E.
name: Hendry, Euan
Publisher: name: arXiv
Year: 2015
Subjects: subject: Optics (physics.optics)
subject: Mesoscale and Nanoscale Physics (cond-mat.mes-hall)
subject: FOS: Physical sciences

description: Here we present an all-optical plasmon coupling scheme, utilising the intrinsic nonlinear optical response of graphene. We demonstrate coupling of free-space, visible light pulses to the surface plasmons in a planar, un-patterned graphene sheet by using nonlinear wave mixing to match both the wavevector and energy of the surface wave. By carefully controlling the phase-matching conditions, we show that one can excite surface plasmons with a defined wavevector and direction across a large frequency range, with an estimated photon efficiency in our experiments approaching $10^{-5}$.
description: 27 pages, 12 figures, includes supplementary material
(2047) Fanny Bates
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Both p16 and p21 Families of Cyclin-dependent Kinase (CDK) Inhibitors Block the Phosphorylation of Cyclin-dependent Kinases by the CDK-activating Kinase
Creators: name: Xiong, Y.
name: Aprelikova, O.
name: Liu, E. T.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: Phosphorylation of cyclin-dependent kinases (CDKs) by the CDK-activating kinase is required for the activation of CDK enzymes. Members of two families of CDK inhibitors, p16/p18 and p21/p27, become physically associated with and inhibit the activity of CDKs in response to a variety of growth-modulating signals. Here, we show that the representative members of both families of CDK inhibitors, p21waf1,cip1, p27kip1, and p18, can prevent the phosphorylation of their CDK partners, CDK2 and CDK6, by CDK-activating kinase. No direct interaction between CDK-activating kinase and the CDK inhibitors could be detected, suggesting that binding of these CDK inhibitors to CDK subunits renders CDK inaccessible to the CDK-activating kinase phosphorylation. These findings suggest that a general mechanism of CDK inhibitor function is to block the phosphorylation of CDK enzymes by CDK-activating kinase.
Additional file 1 of Exploring experiences and needs of spousal carers of people with behavioural variant frontotemporal dementia (bvFTD) including those with familial FTD (fFTD): a qualitative study
Creators: name: Tookey, Sara
name: Greaves, Caroline V.
name: Rohrer, Jonathan D.
name: Desai, Roopal
name: Stott, Joshua
Publisher: name: figshare
Year: 2022
Subjects: subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Science Policy
subject: 111714 Mental Health
subject: FOS: Health sciences

description: Additional file1: AppendixA. Interview Schedule
Data-driven classification of individual cells by their non-Markovian motion
Creators: name: Klimek, Anton
name: Mondal, Debasmita
name: Block, Stephan
name: Sharma, Prerna
name: Netz, Roland R.
Publisher: name: Freie Universität Berlin
Year: 2024
Subjects: subject: Data-driven classification
subject: individual cells
subject: non-Markovian motion
subject: 500 Naturwissenschaften und Mathematik::570 Biowissenschaften; Biologie::570 Biowissenschaften; Biologie

description: We present a method to differentiate organisms solely by their motion based on the generalized Langevin equation (GLE) and use it to distinguish two different swimming modes of strongly confined unicellular microalgae Chlamydomonas reinhardtii. The GLE is a general model for active or passive motion of organisms and particles that can be derived from a time-dependent general many-body Hamiltonian and in particular includes non-Markovian effects (i.e., the trajectory memory of its past). We extract all GLE parameters from individual cell trajectories and perform an unbiased cluster analysis to group them into different classes. For the specific cell population employed in the experiments, the GLE-based assignment into the two different swimming modes works perfectly, as checked by control experiments. The classification and sorting of single cells and organisms is important in different areas; our method, which is based on motion trajectories, offers wide-ranging applications in biology and medicine.
Per the website instructions (http://rspb.royalsocietypublishing.org/final-file-submission-guidelines), all but 1 ESM file was combined into a single file with captions provided for each separate figure, table, or document. from The role of dietary competition in the origination and early diversification of North American euprimates.
Creators: name: Stroik, Laura K.
name: Schwartz, Gary T.
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: Evolutionary Biology
subject: FOS: Biological sciences
subject: 40308 Palaeontology (incl. Palynology)
subject: FOS: Earth and related environmental sciences

description: The conditions under which early euprimates (adapids and omomyids) originated and evolved is an area of longstanding debate. The leading hypotheses of euprimate origins promulgate diet as a core component of the early evolution of this group, despite the role of dietary competition in euprimate originations never being tested directly. This study compared three competition models (non-competition, competitive displacement, competitive coexistence) with observed patterns of dietary niche overlap, reconstructed from three-dimensional molar morphology, at the time of the euprimate radiation in North America (at the Paleocene–Eocene boundary). Overlap of reconstructed multidimensional dietary niches between euprimates and members of their guild were analysed using a modified MANOVA to establish the nature of the competitive environment surrounding euprimate origins in North America (an immigration event). Results indicated that adapids entered the mammalian guild in the absence of competition, suggesting dietary adaptations that were unique within the community. Conversely, omomyids experienced strong, but transitory, competition with nyctitheriids, suggesting that omomyids possessed the ability to out-compete this group. These results show that adapids and omomyids experienced different competitive scenarios upon their arrival (origination) in North America and confirms the significance of diet (and dietary adaptations) in euprimate origination and early diversification in mammalian communities.
In situ Patch-seq analysis of microglia reveals a lack of stress genes as found in FACS-isolated microglia
Creators: name: Bakina, Olga
name: Conrad, Thomas
name: Mitic, Nina
name: Oliveira Vidal, Ramon
name: Obrusnik, Tessa
name: Sai, Somesh
name: Nolte, Christiane
name: Semtner, Marcus
name: Kettenmann, Helmut
Publisher: name: Freie Universität Berlin
Year: 2024
Subjects: subject: Microglial cells
subject: Pipettes
subject: Hippocampus
subject: 500 Naturwissenschaften und Mathematik::540 Chemie::540 Chemie und zugeordnete Wissenschaften

description: We applied the patch-seq technique to harvest transcripts from individual microglial cells from cortex, hippocampus and corpus callosum of acute brain slices from adult mice. After recording membrane currents with the patch-clamp technique, the cytoplasm was collected via the pipette and underwent adapted SMART-seq2 preparation with subsequent sequencing. On average, 4138 genes were detected in 113 cells from hippocampus, corpus callosum and cortex, including microglia markers such as Tmem119, P2ry12 and Siglec-H. Comparing our dataset to previously published single cell mRNA sequencing data from FACS-isolated microglia indicated that two clusters of cells were absent in our patch-seq dataset. Pathway analysis of marker genes in FACS-specific clusters revealed association with microglial activation and stress response. This indicates that under normal conditions microglia in situ lack transcripts associated with a stress-response, and that the microglia-isolation procedure by mechanical dissociation and FACS triggers the expression of genes related to activation and stress.
(2048) George Bates
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
title: Patient preferences of genomic testing in precision cancer medicine
Creators: name: Fulton, Ben Alexander
Publisher: name: University of Glasgow
Year: 2020
Subjects: subject: precision medicine, cancer, patient preferences.

description: Aims: The aim of this thesis was to identify and rate themed patient preference attributes of genomic testing in precision cancer medicine (PCM). The effect of clinical treatment intent and time since completing treatment was examined as a novel hypothesis that these factors influence identified preference attribute themes and/or ratings. This thesis then benchmarked the identified preference attributes against the ATLANTIS clinical trial design, in order to assess how a current clinical trial incorporates patient preferences. Methods: A narrative review of current cancer treatment paradigms was undertaken alongside systematic review of the literature assessing patient preferences of genomic testing in PCM. In addition, mixed methods research, using Nominal Group Technique (NGT), identified and rated preference attribute themes of genomic testing amongst cancer patients. These preference attributes were then benchmarked against genomic testing undertaken within the ATLANTIS clinical trial, to determine how a novel PCM study design incorporated the attributes. Results: Patient preferences of genomic testing in PCM are influenced by clinical treatment intent and time since completing treatment. Patients undergoing cancer treatment with radical intent demonstrated higher preference ratings for test sensitivity (true positive) and specificity (true negative). Invasiveness of testing and test turnaround time were higher rated preference attributes amongst patients undergoing treatment with palliative intent. Ten preference attribute themes of genomic testing were identified: regulatory/NHS approval, test turnaround time, invasiveness of testing, physician approval, test sensitivity (true positive), test specificity (true negative), prevalence of variant, distance to travel, implications for family and family endorsement for testing. The novel adaptive design of the ATLANTIS trial incorporated many of the preference attribute themes of genomic testing demonstrated in this thesis. Conclusions: Patient preferences of genomic testing in PCM are influenced by clinical treatment intent. This thesis identified and rated preference attribute themes of genomic testing for patients, as well as benchmarking these against a current UK PCM clinical trial. The adaptive design of the ATLANTIS trial incorporated many of the preference attributes, but does not allow for assessment of interaction between multiple inter-related attributes. The results of this thesis augment novel clinical trial design for studies incorporating genomic testing in order they retain patient-centred values at their core.
Ceramide Activates the Stress-activated Protein Kinases
Creators: name: Westwick, J. K.
name: Hannun, Y. A.
name: Dbaibo, G.
name: Bielawska, A. E.
name: Brenner, D. A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: Tumor necrosis factor alpha (TNF alpha) activates the stress-activated protein kinases (SAPKs, also known as Jun nuclear kinases or JNKs) resulting in the stimulation of AP-1-dependent gene transcription and induces the translocation of NF kappa B to the nucleus resulting in the stimulation of NF kappa B-dependent gene transcription. A potential second messenger for these signaling pathways is ceramide, which is generated when TNF alpha activates sphingomyelinases. We show that treatment of HL-60 human promyelocytic cells with exogenous sphingomyelinase leads to rapid stimulation of JNK/SAPK activity, an effect not mimicked by treatment with phospholipase A2, C, or D. Further, JNK/SAPK activity is stimulated 2.7- and 2.8-fold, respectively, in cells exposed to C2-ceramide (5 microM) or TNF alpha (10 ng/ml). The prolonged stimulation of this kinase activity by C2-ceramide is similar to that previously reported for TNF alpha. In contrast, the related mitogen-activated protein kinases ERK1 and ERK2 are weakly stimulated following TNF alpha treatment (1.5-fold) and are inhibited by C2-ceramide treatment. TNF alpha also potently stimulates NF-kappa B DNA binding activity and transcriptional activity, but these effects are not mimicked by addition of C2-ceramide or sphingomyelinase to intact cells. Furthermore, TNF alpha, sphingomyelinase, and C2-ceramide induce c-jun, a gene that is stimulated by the ATF-2 and c-Jun transcription factors. These data suggest that ceramide may act as a second messenger for a subset of TNF alpha's biochemical and biological effects.
IPCC Data Distribution Centre
Creators: name: Stockhause, Martina
Publisher: name: Zenodo
Year: 2022
Subjects: Data Distribution Centre
DDC
IPCC FAIR data

description: The introduction to the IPCC Data Distribution Centre was recorded for the IPCC Interactive Atlas Regional Webinars and is part of the outreach materials of the Task Group on Data Support for Climate Change Assessments (TG-Data).
Dataset 3 key from Phylogenetic and functional evidence suggests that deep-ocean ecosystems are highly sensitive to environmental change and direct human disturbance
Creators: name: Ashford, Oliver S.
name: Kenny, Andrew J.
name: Froján, Christopher R. S. Barrio
name: Bonsall, Michael B.
name: Horton, Tammy
name: Brandt, Angelika
name: Bird, Graham J.
name: Gerken, Sarah
name: Rogers, Alex D.
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: Environmental Science
subject: Ecology
subject: FOS: Biological sciences

description: Key to references cited in Dataset 3.
Additional file 1 of “We assist the health system doing the work that should be done by others” – a qualitative study on experiences of grassroots level organizations providing refugee health care during the 2015 migration event in Germany
Creators: name: Brenner, Stephan
name: Lok, Vincent
Publisher: name: figshare
Year: 2022
Subjects: subject: Law
subject: FOS: Law

description: Additional file 1.
(2049) Aked Bates
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Barjols, hôtel du Pont d'or, le félibre Gabriel Larose, fondateur de l'Escolo Caramy de Brignoles, répond aux questions de Claudie Marcel-Dubois
Creators: name: Musée National Des Arts Et Traditions Populaires (Paris)
name: Centre D'ethnologie Française
Publisher: name: École des Hautes Études en Sciences Sociales; Institut  interdisciplinaire d'anthropologie du contemporain (Paris); Laboratoire d'anthropologie et d'histoire de l'institution de la culture (Paris)
Year: 2024

Abstract
Characterization of the Human Inosine-5′-monophosphate Dehydrogenase Type II Gene
Creators: name: Mitchell, Beverly S.
name: Zimmermann, Albert G.
name: Spychalal, Jozef
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: Inosine-5'-monophosphate dehydrogenase (IMPDH) activity and mRNA levels are induced up to 15-fold upon mitogenic or antigenic stimulation of human peripheral blood T lymphocytes. This increase in IMPDH activity is required for cellular proliferation and has been associated with malignant transformation. We have cloned the human IMPDH type II gene and show that it contains 14 exons and is approximately 5.8 kilobases in length. Exons vary in size from 49 to 207 base pairs and introns from 73 to 1065 base pairs. The transcription start site was mapped to a position 50 nucleotides upstream of the translation initiation site. The 5'-flanking region consisting of 463 base pairs upstream of the translation initiation site confers induced transcription and differential regulation upon a chloramphenicol acetyltransferase reporter gene when transfected into Jurkat T cells and human peripheral blood T lymphocytes, respectively. DNase I footprinting analysis using Jurkat T cell nuclear extract identified four protected regions in the promoter which coincide with consensus transcription factor binding sites for the nuclear factors AP2, ATF, CREB, Egr-1, Nm23, and Sp1. These findings suggest that several of these nuclear factors may play a critical role in the regulation of IMPDH type II gene expression during T lymphocyte activation.
A NuSTAR observation of the eclipsing binary system OAO 1657-415: The revival of the cyclotron line
Creators: name: Saavedra, Enzo A.
name: Fogantini, Federico A.
name: Combi, Jorge A.
name: García, Federico
name: Chaty, Sylvain
Publisher: name: arXiv
Year: 2021
Subjects: subject: High Energy Astrophysical Phenomena (astro-ph.HE)
subject: FOS: Physical sciences

description: OAO 1657-415 is an accreting X-ray pulsar with a high mass companion that has been observed by several telescopes over the years, in different orbital phases. Back in 1999, observations performed with Beppo-SAX lead to the detection of a cyclotron-resonant-scattering feature, which has not been found again with any other instrument. A recent NuSTAR X-ray observation, performed during the brightest phase of the source, allows us to perform sensitive searches for cyclotron-resonant-scattering features in the hard X-ray spectrum of the source. We aim to characterise the source by means of temporal and spectral X-ray analysis, and to confidently search for the presence of cyclotron-resonant-scattering features. The observation was divided into four time intervals in order to characterise each one. Several timing analysis tools were used to obtain the pulse of the neutron star, and the light curves folded into the time intervals. The NuSTAR spectrum in the energy range 3-79 keV was used, which was modelled with a power-law continuum emission model with a high-energy cutoff. We find the pulsations associated with the source in the full observation, which are shifted due to the orbital Doppler effect. We show evidence that a cyclotron line at $35.6 \pm 2.5$ keV is present in the spectrum. We use this energy to estimate the dipolar magnetic field at the pulsar surface to be $4.0\pm 0.2\,\times\,10^{12}$ G. We further estimate a lower limit in the distance to OAO 1657-415 of $\simeq 1$ kpc. And we also find a possible positive correlation between the luminosity and the energy associated with the cyclotron line. We conclude that the cyclotron line at $35.6\pm2.5$ keV is the same as that detected by Beppo-SAX. Our detection has a significance of $\sim$ 3.4$σ$.
description: 9 pages, 9 figures. Accepted for publication in A&A
Per the website instructions (http://rspb.royalsocietypublishing.org/final-file-submission-guidelines), all but 1 ESM file was combined into a single file with captions provided for each separate figure, table, or document. from The role of dietary competition in the origination and early diversification of North American euprimates
Creators: name: Stroik, Laura K.
name: Schwartz, Gary T.
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: Evolutionary Biology
subject: FOS: Biological sciences
subject: 40308 Palaeontology (incl. Palynology)
subject: FOS: Earth and related environmental sciences

description: The conditions under which early euprimates (adapids and omomyids) originated and evolved is an area of longstanding debate. The leading hypotheses of euprimate origins promulgate diet as a core component of the early evolution of this group, despite the role of dietary competition in euprimate originations never being tested directly. This study compared three competition models (non-competition, competitive displacement, competitive coexistence) to observed patterns of dietary niche overlap, reconstructed from three-dimensional molar morphology, at the time of the euprimate radiation in North America (at the Paleocene–Eocene boundary). Overlap of reconstructed multidimensional dietary niches between euprimates and members of their guild were analysed using a modified MANOVA to establish the nature of the competitive environment surrounding euprimate origins in North America (an immigration event). Results indicated that adapids entered the mammalian guild in the absence of competition, suggesting dietary adaptations that were unique within the community. Conversely, omomyids experienced strong, but transitory, competition with nyctitheriids, suggesting that omomyids possessed the ability to out-compete this group. These results show that adapids and omomyids experienced different competitive scenarios upon their arrival (origination) in North America and confirms the significance of diet (and dietary adaptations) in euprimate origination and early diversification in mammalian communities.
Cloning and Expression of Mitochondrial Translational Elongation Factor Ts from Bovine and Human Liver
Creators: name: Woriax, V.
name: Xin, H.
name: Spremulli, L. L.
name: Burkhart, W.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: The sequences of the cDNAs for the mitochondrial translational elongation factor Ts (EF-Tsmt) from bovine and human liver have been obtained. The deduced amino acid sequence of bovine liver EF-Tsmt is 338 residues in length and includes a 55-amino acid signal peptide and a mature protein of 283 residues. The sequence of the mature form of bovine EF-Tsmt is 91% identical to that of human EF-Tsmt and 29% identical to Escherichia coli EF-Ts. Southern analysis indicates that there are two genes for EF-Tsmt in bovine liver chromosomal DNA. A 224-base pair intron is located near the 5'-end of at least one of these genes. Northern analysis using a human multiple tissue blot indicates that EF-Tsmt is expressed in all tissues, with the highest levels of expression in skeletal muscle, liver, and kidney. Both the mature and precursor forms of bovine liver EF-Tsmt have been expressed in E. coli as histidine-tagged proteins. The mature form of EF-Tsmt forms a complex with E. coli elongation factor Tu. This complex is active in poly(U)-directed polymerization of phenylalanine. The precursor form is expressed as a 42-kDa protein, which is rapidly degraded in the cell.
(2045) Henry Timothy Bates
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
ESM Section 1 from Two types of social grooming methods depending on the trade-off between the number and strength of social relationships.
Creators: name: Takano, Masanori
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: 179999 Psychology and Cognitive Sciences not elsewhere classified
subject: FOS: Psychology
subject: 80201 Analysis of Algorithms and Complexity
subject: FOS: Computer and information sciences

description: Data-Sets section
Quantum-State-Sensitive Detection of Alkali Dimers on Helium Nanodroplets by Laser-Induced Coulomb Explosion
Creators: name: Kristensen, Henrik H.
name: Kranabetter, Lorenz
name: Schouder, Constant A.
name: Stapper, Christoph
name: Arlt, Jacqueline
name: Mudrich, Marcel
name: Stapelfeldt, Henrik
Publisher: name: arXiv
Year: 2021
Subjects: subject: Atomic and Molecular Clusters (physics.atm-clus)
subject: Chemical Physics (physics.chem-ph)
subject: Optics (physics.optics)
subject: FOS: Physical sciences

description: Rubidium dimers residing on the surface of He nanodroplets are doubly ionized by an intense fs laser pulse leading to fragmentation into a pair of $\mathrm{Rb^+}$ ions. We show that the kinetic energy of the $\mathrm{Rb^+}$ fragment ions can be used to identify dimers formed in either the X $^1Σ_{\mathrm{g}}^+$ ground state or in the lowest-lying triplet state, a $^3Σ_{\mathrm{u}}^+$. From the experiment, we estimate the abundance ratio of dimers in the a and X states as a function of the mean droplet size and find values between 4:1 and 5:1. Our technique applies generally to dimers and trimers of alkali atoms, here also demonstrated for $\mathrm{Li_2}$, $\mathrm{Na_2}$, and $\mathrm{K_2}$, and will enable fs time-resolved measurements of their rotational and vibrational dynamics, possibly with atomic structural resolution.
description: 5 pages, 4 figures
Cloning and Sequence Analysis of the Human Mitochondrial Translational Initiation Factor 2 cDNA
Creators: name: Ma, L.
name: Spremulli, L. L.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: Complete cDNAs encoding human mitochondrial translational initiation factor 2 (IF-2mt) have been obtained from liver, heart, and fetal brain cDNA libraries. These cDNAs have a long open reading frame 2181 residues in length encoding a protein of 727 amino acids. Overall, human IF-2mt has 30-40% identity to the corresponding prokaryotic factors. Surprisingly, it is no more homologous to yeast IF-2mt than to the IF-2s from bacterial sources. The greatest region of conservation lies in the G-domain of this factor with less conservation in the COOH-terminal half of the protein and very little homology near the amino terminus. The 5'-untranslated leaders of the liver and heart cDNAs contain a number of short open reading frames. These sequences may play a role in the translational activity of the IF-2mt mRNA. Northern analysis indicates that the IF-2mt gene is expressed in all tissues but that the level of expression varies over a wide range.
Correlation of Real-time Catecholamine Release and Cytosolic Ca 2+ at Single Bovine Chromaffin Cells
Creators: name: Wightman, R. Mark
name: Finnegan, Jennifer M.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: Previous investigations of the role of Ca2+ in stimulus-secretion coupling have been undertaken in populations of adrenal chromaffin cells. In the present study, the simultaneous detection of intracellular Ca2+, with the fluorescent probe fura-2, and catecholamine release, using a carbon-fiber microelectrode, are examined at single chromaffin cells in culture. Results from classic depolarizing stimuli, high potassium (30-140 mM) and 1,1-dimethyl-4-phenylpiperazinium (3-50 microM), show a dependence of peak cytosolic Ca2+ concentration and catecholamine release on secretagogue concentration. Catecholamine release induced by transient high K+ stimulation increases logarithmically with K+ concentration. Continuous exposure to veratridine (50 microM) induces oscillations in intracellular Ca2+ and at higher concentrations (100 microM) concomitant fluctuation of cytosolic Ca2+ and catecholamine secretion. Mobilization of both caffeine- and inositol trisphosphate-sensitive intracellular Ca2+ stores is found to elicit secretion with or without extracellular Ca2+. Caffeine-sensitive intracellular Ca2+ stores can be depleted, refilled, and cause exocytosis in medium without Ca2+. Single cell measurement of exocytosis and the increase in cytosolic Ca2+ induced by bradykinin-activated intracellular stores reveal cell to cell variability in exocytotic responses which is masked in populations of cells. Taken together, these results show that exocytosis of catecholamines can be induced by an increase in cytosolic Ca2+ either as a result of transmembrane entry or by release of internal stores.
(2042) Clara Beardshaw
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Design of a Ruthenium-Cytochrome c Derivative to Measure Electron Transfer to the Initial Acceptor in Cytochrome c Oxidase
Creators: name: Fine, Bryan R.
name: Geren, Lois M.
name: Millett, Francis
name: Beasley, James R.
name: Saunders, Aleister J.
name: Pielak, Gary J.
name: Hibdon, Sharon
name: Durham, Bill
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: A ruthenium-labeled cytochrome c derivative was prepared to meet two design criteria: the ruthenium group must transfer an electron rapidly to the heme group, but not alter the interaction with cytochrome c oxidase. Site-directed mutagenesis was used to replace His39 on the backside of yeast C102T iso-1-cytochrome c with a cysteine residue, and the single sulfhydryl group was labeled with (4-bromomethyl-4' methylbipyridine) (bis-bipyridine)ruthenium(II) to form Ru-39-cytochrome c (cyt c). There is an efficient pathway for electron transfer from the ruthenium group to the heme group of Ru-39-cyt c comprising 13 covalent bonds and one hydrogen bond. Electron transfer from the excited state Ru(II*) to ferric heme c occurred with a rate constant of (6.0 +/- 2.0) x 10(5) s-1, followed by electron transfer from ferrous heme c to Ru(III) with a rate constant of (1.0 +/- 0.2) x 10(6) s-1. Laser excitation of a complex between Ru-39-cyt c and beef cytochrome c oxidase in low ionic strength buffer (5 mM phosphate, pH7) resulted in electron transfer from photoreduced heme c to CuA with a rate constant of (6 +/- 2) x 10(4) s-1, followed by electron transfer from CuA to heme a with a rate constant of (1.8 +/- 0.3) x 10(4) s-1. Increasing the ionic strength to 100 mM leads to bimolecular kinetics as the complex is dissociated. The second-order rate constant is (2.5 +/- 0.4) x 10(7) M-1s-1 at 230 mM ionic strength, nearly the same as that of wild-type iso-1-cytochrome c.
Horologium II: a Second Ultra-faint Milky Way Satellite in the Horologium Constellation
Creators: name: Kim, Dongwon
name: Jerjen, Helmut
Publisher: name: arXiv
Year: 2015
Subjects: subject: Astrophysics of Galaxies (astro-ph.GA)
subject: FOS: Physical sciences

description: We report the discovery of a new ultra-faint Milky Way satellite candidate, Horologium II, detected in the Dark Energy Survey Y1A1 public data. Horologium II features a half light radius of $r_{h}=47\pm10$ pc and a total luminosity of $M_{V}=-2.6^{+0.2}_{-0.3}$ that place it in the realm of ultra-faint dwarf galaxies on the size-luminosity plane. The stellar population of the new satellite is consistent with an old ($\sim13.5$ Gyr) and metal-poor ([Fe/H]$\sim-2.1$) isochrone at a distance modulus of $(m-M)=19.46\pm0.20$, or a heliocentric distance of $78\pm8$ kpc, in the color-magnitude diagram. Horologium II has a distance similar to the Sculptor dwarf spheroidal galaxy ($\sim82$ kpc) and the recently reported ultra-faint satellites Eridanus III ($87\pm8$ kpc) and Horologium I ($79\pm8$ kpc). All four satellites are well aligned on the sky, which suggests a possible common origin. As Sculptor is moving on a retrograde orbit within the Vast Polar Structure when compared to the other classical MW satellite galaxies including the Magellanic Clouds, this hypothesis can be tested once proper motion measurements become available.
description: 5 pages, 3 figures, 1 table. Accepted for publication in ApJL. (w.r.t. v1: figures updated; minor changes throughout the text)
Evidence for an Anti-parallel Orientation of the Ligand-activated Human Androgen Receptor Dimer
Creators: name: Zhou, Zhoug Xun
name: Wilson, Elizabeth M.
name: Langley, Elizabeth
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: Domain interactions of the human androgen receptor (AR) dimer were investigated using a protein-protein interaction assay in which the NH2- and carboxyl-terminal regions of human AR were fused to the Saccharomyces cerevisiae GAL4 DNA-binding domain and herpes simplex virus VP16 transactivation domain to produce chimeric proteins. Transcriptional activation of a GAL4 luciferase reporter vector up to 100-fold was greater than Fos/Jun leucine zipper binding, indicating stable AR interaction between AR NH2-terminal residues 1-503 and steroid-binding domain residues 624-919 that was specific for and dependent on androgen binding to the steroid-binding domain and was inhibited by anti-androgen binding. Deletion mutagenesis within the NH2-terminal region indicated transactivation domain residues 142-337 were not required for dimerization, whereas deletions near the NH2 terminus (delta 14-150) or NH2-terminal to the DNA-binding domain (delta 339-499) reduced or eliminated the AR interaction, respectively. An NH2-/NH2-terminal interaction was also observed, but no interaction was detected between ligand-free or bound steroid-binding domains. The results indicate that high affinity androgen binding promotes interactions between the NH2-terminal and steroid-binding domains of human AR, raising the possibility of an androgen-induced anti-parallel AR dimer.
(2043) Joseph Bates
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
ESM Section 1 from Two types of social grooming methods depending on the trade-off between the number and strength of social relationships
Creators: name: Takano, Masanori
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: 179999 Psychology and Cognitive Sciences not elsewhere classified
subject: FOS: Psychology
subject: 80201 Analysis of Algorithms and Complexity
subject: FOS: Computer and information sciences

description: Data-Sets section
(2044) Elizabeth Clark
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
ESM Table 2 from Two types of social grooming methods depending on the trade-off between the number and strength of social relationships.
Creators: name: Takano, Masanori
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: 179999 Psychology and Cognitive Sciences not elsewhere classified
subject: FOS: Psychology
subject: 80201 Analysis of Algorithms and Complexity
subject: FOS: Computer and information sciences

description: The results of the linear regression models in Fig. 11b. All p-values of coefficients of a (β1) were significant (at the 5% level).