Dengue-specific subviral nanoparticles: Design, creation and characterization
Creators: name: Khetarpal, Niyati
name: Poddar, Ankur
name: Nemani, Satish K.
name: Dhar, Nisha
name: Patil, Aravind
name: Negi, Priyanka
name: Perween, Ashiya
name: Viswanathan, Ramaswamy
name: Lünsdorf, Heinrich
name: Tyagi, Poornima
name: Raut, Rajendra
name: Arora, Upsana
name: Jain, Swatantra K.
name: Rinas, Ursula
name: Swaminathan, Sathyamangalam
name: Khanna, Navin
Publisher: name: London : BioMed Central Ltd.
Year: 2013
Subjects: subject: Bionanoparticles
subject: Dengue envelope domain III
subject: Hepatitis B surface antigen
subject: Pichia pastoris
subject: Virus-like particle
subject: Envelope domains
subject: Pichia Pastoris
subject: Virus-like particles
subject: Antigens
subject: Diagnosis
subject: Medical applications
subject: Viruses
subject: antigen
subject: hepatitis B surface antigen
subject: nanoparticle
subject: s antigen
subject: unclassified drug
subject: virus envelope protein
subject: cell extract
subject: hybrid protein
subject: S envelope protein, hepatitis B virus
subject: virus antigen
subject: virus protein
subject: article
subject: cell lysate
subject: Dengue virus
subject: gene dosage
subject: gene expression
subject: molecular cloning
subject: nonhuman
subject: protein domain
subject: virus like agent
subject: animal
subject: chemistry
subject: Chlorocebus aethiops
subject: dengue
subject: isolation and purification
subject: metabolism
subject: physiology
subject: Pichia
subject: protein tertiary structure
subject: species difference
subject: ultrastructure
subject: Vero cell line
subject: virion
subject: virology
subject: Hepatitis B virus
subject: Animals
subject: Antigens, Viral
subject: Cell Extracts
subject: Cercopithecus aethiops
subject: Dengue
subject: Dengue Virus
subject: Nanoparticles
subject: Protein Structure, Tertiary
subject: Recombinant Fusion Proteins
subject: Species Specificity
subject: Vero Cells
subject: Viral Envelope Proteins
subject: Viral Proteins
subject: Virion
subject: Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
subject: Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit

description: Background: Dengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like particles (VLP) offer a versatile nanoscale platform for developing tools with potential biomedical applications. From the perspective of a potentially useful dengue-specific tool, the dengue virus envelope protein domain III (EDIII), endowed with serotype-specificity, host receptor recognition and the capacity to elicit virus-neutralizing antibodies, is an attractive candidate.Methods: We have developed a strategy to co-express and co-purify Hepatitis B virus surface (S) antigen in two forms: independently and as a fusion with EDIII. We characterized these physically and functionally.Results: The two forms of the S antigen associate into VLPs. The ability of these to display EDIII in a functionally accessible manner is dependent upon the relative levels of the two forms of the S antigen. Mosaic VLPs containing the fused and un-fused components in 1:4 ratio displayed maximal functional competence.Conclusions: VLPs armed with EDIII may be potentially useful in diagnostic, therapeutic and prophylactic applications.