Limosilactobacillus reuteri SUBSP. MURIUM 2021, SUBSP. NOV.
Creators: name: Li, Fuyong
name: Cheng, Christopher C.
name: Zheng, Jinshui
name: Liu, Junhong
name: Quevedo, Rodrigo Margain
name: Li, Junjie
name: Roos, Stefan
name: Gänzle, Michael G.
name: Walter, Jens
Publisher: name: Zenodo
Year: 2021
Subjects: Biodiversity
Taxonomy
Bacteria
Firmicutes
Bacilli
Lactobacillales
Lactobacillaceae
Limosilactobacillus
Limosilactobacillus reuteri

description: DESCRIPTION OF LIMOSILACTOBACILLUS REUTERI SUBSP. MURIUM SUBSP. NOV.   
 Limosilactobacillus reuteri subsp. murium (mu′ ri.um. L. plur. gen. n. murium of mice, referring to the adaptation of strains of the subspecies to rodents including mice).   
 L. reuteri strains clustered in lineage I (Fig. 3) belong to L. reuteri subsp. murium and they were isolated from rodents [5 – 7]. Strains of this subspecies have ANI values of 96.8–99.1 % with each other and ANI values of 94.5–96.5% with other L. reuteri strains belonging to different subspecies (Fig. 4). Acid is produced from L-arabinose,D-ribose, D-galactose,D-glucose, maltose, lactose, melibiose, sucrose and raffinose; acid production from potassium gluconate is strain-specific; acid is not produced from D-xylose, D-fructose, D-mannose, methyl α- D-glucopyranoside, aesculin, glycerol, erythritol, D-arabinose, L-xylose, D-adonitol, methyl β -D-xylopyranoside, L-sorbose, L-rhamnose, dulcitol, inositol, D-mannitol, D-sorbitol, methyl α- D-mannopyranoside, N-acetylglucosamine, amygdalin, arbutin, salicin, cellobiose, trehalose, inulin, melezitose, starch, glycogen, xylitol, gentiobiose, turanose, D-lyxose,D-tagatose,D-fucose,L-fucose,D-arabitol, L-arabitol, potassium 2-ketogluconate or potassium 5-ketogluconate. Phylogenetic analyses based on the core genes identified in this study (Fig. 3) and a previous studies [5], AFLP and MLSA (using concatenated sequences of ddl, pkt, leuS, gyrB, dltA, rpoA and recA genes) [7] indicate that strains clustered in this lineage are rodent-specific. Strains of L. reuteri subsp. murium displayed elevated fitness in mice through the colonization and biofilm formation on the forestomach epithelium [5, 7, 11], suggesting that their evolution with rodents was adaptive and led to host specificity. Large surface proteins (>750 aa) exist among strains belonging to this subspecies, which involve in epithelial adhesion and biofilm formation [6]. Strains of this subspecies produce the enzyme urease for acid resistance and rarely produce the antimicrobial compound reuterin [6, 8].   
 The type strain, lpuph1 T (=DSM 110570 T =LMG 31634 T), was isolated from mouse gastrointestinal tract [6, 7], with a DNA G+C content of 38.4mol%.
description: Published as part of Li, Fuyong, Cheng, Christopher C., Zheng, Jinshui, Liu, Junhong, Quevedo, Rodrigo Margain, Li, Junjie, Roos, Stefan, Gänzle, Michael G. & Walter, Jens, 2021, Limosilactobacillus balticus sp. nov., Limosilactobacillus agrestis sp. nov., Limosilactobacillus albertensis sp. nov., Limosilactobacillus rudii sp. nov. and Limosilactobacillus fastidiosus sp. nov., five novel Limosilactobacillus species isolated from the vertebrate gastrointestinal tract, and proposal of six subspecies of Limosilactobacillus reuteri adapted to the gastrointestinal tract of specific vertebrate hosts, pp. 1-21 in International Journal of Systematic and Evolutionary Microbiology (004644) (004644) 71 (2) on page 19, DOI: 10.1099/ijsem.0.004644, http://zenodo.org/record/6048739