title: ÐÑÐ¾Ð±ÐµÐ½Ð½Ð¾ÑÑÐ¸ Ð¿ÑÐ¾ÑÐµÐºÐ°Ð½Ð¸Ñ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸ÑÐ¾ÑÐ¼Ð½Ð¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð² Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ðµ ÐºÑÑÑ Ð½Ð° ÑÐ°Ð·Ð½ÑÑ ÑÑÐ°Ð´Ð¸ÑÑ Ð¾Ð½ÑÐ¾Ð³ÐµÐ½ÐµÐ·Ð°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Features of the progression of epileptiform activity in the rat hippocampus at different stages of ontogenesis
Creators: name: ÐÐ¸Ð»Ð»Ð¾, ÐÐ°Ð»ÐµÐ½ÑÐ¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿
Ð²Ð¸ÑÐ¾ÑÐ½Ð°Ñ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ñ
4-Ð°Ð¼Ð¸Ð½Ð¾Ð¿Ð¸ÑÐ¸Ð´Ð¸Ð½Ð¾Ð²Ð°Ñ Ð¼Ð¾Ð´ÐµÐ»Ñ
Ð²Ð¾Ð·ÑÐ°ÑÑÐ½ÑÐµ Ð¾ÑÐ¾Ð±ÐµÐ½Ð½Ð¾ÑÑÐ¸
hippocampus
temporal lobe epilepsy
4-aminopyridine model
age-related features

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð²Ð¾Ð·ÑÐ°ÑÑÐ½ÑÑ Ð¾ÑÐ¾Ð±ÐµÐ½Ð½Ð¾ÑÑÐµÐ¹ Ð¿ÑÐ¾ÑÐµÐºÐ°Ð½Ð¸Ñ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸ÑÐ¾ÑÐ¼Ð½Ð¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ (Ð­Ð) Ð² Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ðµ ÐºÑÑÑ, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð²Ð¾Ð·ÑÐ°ÑÑÐ½Ð¾Ð³Ð¾ Ð¿ÐµÑÐ¸Ð¾Ð´Ð°, Ð² ÐºÐ¾ÑÐ¾ÑÑÐ¹ Ð¿ÑÐ¾Ð¸ÑÑÐ¾Ð´Ð¸Ñ ÑÐ¼ÐµÐ½Ð° Ð¾ÑÐ½Ð¾Ð²Ð½ÑÑ Ð¿Ð°ÑÑÐµÑÐ½Ð¾Ð² Ð­Ð. ÐÐ°Ð´Ð°ÑÐ¸, ÑÐµÑÐ°ÐµÐ¼ÑÐµ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: 1. Ð¡ÑÐ°Ð²Ð½Ð¸ÑÑ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ Ð¿Ð°ÑÑÐµÑÐ½Ñ Ð­Ð Ð² Ð¿Ð¾Ð»Ðµ Ð¡Ð1 Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð° Ñ ÐºÑÑÑ ÑÐ°Ð·Ð½ÑÑ Ð²Ð¾Ð·ÑÐ°ÑÑÐ½ÑÑ Ð¿ÐµÑÐ¸Ð¾Ð´Ð¾Ð²: P5, P7, P10, P12, P14, P18, P21, P60, P120, P180, Ð 270, P360, Ð 540; 2. ÐÑÑÐ²Ð¸ÑÑ ÐºÑÐ¸ÑÐ¸ÑÐµÑÐºÐ¸Ðµ Ð²Ð¾Ð·ÑÐ°ÑÑÐ½ÑÐµ Ð¿ÐµÑÐ¸Ð¾Ð´Ñ, Ð² ÐºÐ¾ÑÐ¾ÑÑÐµ Ð¿ÑÐ¾Ð¸ÑÑÐ¾Ð´Ð¸Ñ ÑÐ¼ÐµÐ½Ð° Ð¿Ð°ÑÑÐµÑÐ½Ð¾Ð² Ð­Ð Ð² Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ðµ. Ð Ð°Ð±Ð¾ÑÐ° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð² ÐÐ­Ð¤Ð Ð. Ð. Ð¡ÐµÑÐµÐ½Ð¾Ð²Ð° Ð ÐÐ Ð² Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½ÑÑ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð¾Ð² Ð½ÐµÐ¹ÑÐ¾Ð½Ð½ÑÑ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ð¹. Ð­Ð»ÐµÐºÑÑÐ¾ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÑ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ñ Ð½Ð° Ð³Ð¾ÑÐ¸Ð·Ð¾Ð½ÑÐ°Ð»ÑÐ½ÑÑ Ð¿ÐµÑÐµÐ¶Ð¸Ð²Ð°ÑÑÐ¸Ñ ÑÑÐµÐ·Ð°Ñ Ð³Ð¾Ð»Ð¾Ð²Ð½Ð¾Ð³Ð¾ Ð¼Ð¾Ð·Ð³Ð° ÐºÑÑÑ ÐÐ¸ÑÑÐ°Ñ. ÐÑÐ²Ð¾Ð´Ð¸Ð»Ð¸ Ð¿Ð¾Ð»ÐµÐ²ÑÐµ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ñ Ð¾Ñ Ð»ÑÑÐ¸ÑÑÐ¾Ð³Ð¾ ÑÐ»Ð¾Ñ Ð¿Ð¾Ð»Ñ Ð¡Ð1 Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð° Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ Ð¸Ð½Ð´ÑÐºÑÐ¸Ð¸ Ð­Ð in vitro. Ð­Ð Ð²ÑÐ·ÑÐ²Ð°Ð»Ð¸ Ð°Ð¿Ð»Ð¸ÐºÐ°ÑÐ¸ÐµÐ¹ Ð¿ÑÐ¾ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐ°ÑÑÐ²Ð¾ÑÐ°, ÑÐ¾Ð´ÐµÑÐ¶Ð°ÑÐµÐ³Ð¾ Ð¿Ð¾Ð½Ð¸Ð¶ÐµÐ½Ð½ÑÑ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ñ Ð¸Ð¾Ð½Ð¾Ð² Mg2+ (0.25 Ð¼Ð), Ð¿Ð¾Ð²ÑÑÐµÐ½Ð½ÑÑ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ñ Ð¸Ð¾Ð½Ð¾Ð² K+ (3.5 Ð¼Ð), Ð¸ ÑÐµÐ¼Ð¾ÐºÐ¾Ð½Ð²ÑÐ»ÑÑÐ°Ð½Ñ 4-Ð°Ð¼Ð¸Ð½Ð¾Ð¿Ð¸ÑÐ¸Ð´Ð¸Ð½ (100 Ð¼ÐºÐ). Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð±ÑÐ»Ð¾ Ð²ÑÑÐ²Ð»ÐµÐ½Ð¾, ÑÑÐ¾ Ð½ÐµÐ¹ÑÐ¾Ð½Ð½ÑÐµ ÑÐµÑÐ¸ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð° Ð´ÐµÐ¼Ð¾Ð½ÑÑÑÐ¸ÑÑÑÑ ÑÐ°Ð·Ð½ÑÐµ Ð¿Ð°ÑÑÐµÑÐ½Ñ Ð­Ð Ñ ÐºÑÑÑ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ Ð²Ð¾Ð·ÑÐ°ÑÑÐ¾Ð². Ð¡ Ð²Ð¾Ð·ÑÐ°ÑÑÐ¾Ð¼ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð¾ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸ÑÐ¾ÑÐ¼Ð½ÑÑ ÑÐ¾Ð±ÑÑÐ¸Ð¹ Ð¸ Ð»Ð°ÑÐµÐ½ÑÐ½ÑÐµ Ð¿ÐµÑÐ¸Ð¾Ð´Ñ Ð²Ð¾Ð·Ð½Ð¸ÐºÐ½Ð¾Ð²ÐµÐ½Ð¸Ñ Ð­Ð ÑÐ²ÐµÐ»Ð¸ÑÐ¸Ð²Ð°ÐµÑÑÑ. 18-ÑÐ¸ Ð´Ð½ÐµÐ²Ð½ÑÐ¹ Ð²Ð¾Ð·ÑÐ°ÑÑ ÑÐ²Ð»ÑÐµÑÑÑ ÐºÑÐ¸ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ ÑÐ¾ÑÐºÐ¾Ð¹ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ñ, Ð² ÐºÐ¾ÑÐ¾ÑÑÐ¹ Ð¿ÑÐ¾Ð¸ÑÑÐ¾Ð´Ð¸Ñ ÑÐ¼ÐµÐ½Ð° Ð¾ÑÐ½Ð¾Ð²Ð½ÑÑ Ð¿Ð°ÑÑÐµÑÐ½Ð¾Ð² Ð­Ð. Ð ÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð¼Ð¾Ð³ÑÑ Ð±ÑÑÑ Ð¿Ð¾Ð»ÐµÐ·Ð½Ñ Ð´Ð»Ñ Ð¿Ð¾Ð½Ð¸Ð¼Ð°Ð½Ð¸Ñ Ð¿ÑÐ¸Ð½ÑÐ¸Ð¿Ð¾Ð² ÑÐ°Ð±Ð¾ÑÑ Ð¼Ð¾Ð·Ð³Ð° Ð¿ÑÐ¸ ÐµÐ³Ð¾ Ð½Ð¾ÑÐ¼Ð°Ð»ÑÐ½Ð¾Ð¼ Ð¸ Ð¿Ð°ÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð¼ ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸.
description: This work is devoted to the study of age-specific features of the course of epileptiform activity (EA) in the rat hippocampus, as well as to the determination of the age period in which the change of the main EA patterns occurs. The objectives of the study are: 1. To compare electrophysiological patterns of EA in the CA1 field of the hippocampus in rats of different age periods: P5, P7, P10, P12, P14, P18, P21, P60, P120, P180, P270, P360, P540; 2. Identify critical age periods in which there is a change of EA patterns in the hippocampus. The work was carried out at the I. M. Sechenov IEFB of the Russian Academy of Sciences in the laboratory of molecular mechanisms of neuronal interactions. Electrophysiological experiments were performed on horizontal surviving brain slices of Wistar rats. Field potentials were withdrawn from the radial layer of the CA1 field of the hippocampus under conditions of EA induction in vitro. EA was induced by the application of a proepileptic solution containing a reduced concentration of Mg2+ ions (0.25 mM), an increased concentration of K+ ions (3.5 mM), and the chemoconvulsant 4-aminopyridine (100 Î¼M). The results revealed that hippocampal neuronal networks exhibit different patterns of EA in rats of different ages. The number of epileptiform events and the latency periods of EA occurrence increase with age. The 18-day-old age is a critical developmental point at which a change in the basic EA patterns occurs. The results may be useful for understanding the principles of brain function in normal and pathological functioning.