title: ÐÐµÑ Ð°Ð½Ð¸Ð·Ð¼Ñ ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ñ Ð¿ÑÐ¾Ð½Ð¸ÑÐ°ÐµÐ¼Ð¾ÑÑÐ¸ Ð¼ÐµÐ¼Ð±ÑÐ°Ð½ Ð² Ð¿ÑÐ¸ÑÑÑÑÑÐ²Ð¸Ð¸ Ð¿Ð¾Ð»Ð¸Ð¼Ð¸ÐºÑÐ¸Ð½Ð° Ð
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Mechanisms for increasing of the membrane permeability in the presence of polymyxin B
Creators: name: Ð¥Ð°Ð»ÐµÐ½ÑÐ²Ð°, ÐÐ°ÑÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: Ð¼Ð¾Ð´ÐµÐ»ÑÐ½ÑÐµ Ð»Ð¸Ð¿Ð¸Ð´Ð½ÑÐµ Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ñ
Ð»Ð¸Ð¿Ð¾ÑÐ¾Ð¼Ñ
Ð¿Ð¾Ð»Ð¸Ð¼Ð¸ÐºÑÐ¸Ð½ Ð²
Ð¼Ð°Ð»ÑÐµ Ð¼Ð¾Ð»ÐµÐºÑÐ»Ñ
model lipid membranes
liposomes
polymyxin b
small molecules

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description: The aim of the work was to study the mechanisms of increasing in the permeability of membranes modified by polymyxin B (PMB), and to study the ways of regulating the membrane activity of PMB by small molecules. The method for reconstruction of the ion channels into planar lipid membranes and the fluorimetry of marker leakage from lipid vesicles in the presence of PMB with and without the small molecules have been performed. It is found that PMB at the concentration up to 3 Î¼M does not change the electric parameter of uncharged and negatively charged lipid bilayers. It is shown that positively charged PMB forms ion-permeable pores at the concentrations from 14 to 20 Î¼M in the negatively charged lipid bilayers compared to the membranes composed of the equimolar mixture of dioleoylphosphoserine and dioleoylphosphoethanolamine or palmitooleoylphosphoglycerol and palmitoooleoylphosphoethanolamine. The introduction of PMB up to 15 Î¼M into solutions bathing uncharged dioleoylphosphocholine bilayers does not induce the step-like current fluctuations. An increase in the concentration of PMB above 15 and 100 Î¼M causes a rupture of the uncharged and charged bilayers respectively. It is demonstrated that the addition of phloretin and triiodothyronine to the negatively charged bilayers causes the 20% and 40% decrease, while the introduction of RH421 and tetracaine was accompanied by the 50% and 20% increase in the PMB-induced steady-state transmembrane current, respectively. It was concluded that the membrane dipole potential plays a significant role in the regulation of the pore-forming activity of PMB.