A potent drug candidature of Cu(II) pyrazino[2,3‐f][1,10]phenanthroline complexes with bioactive ligands: synthesis, crystal structures, biomolecular interactions, radical scavenging and cytotoxicities
Creators: name: İnci, Duygu
name: Aydın, Rahmiye
name: Vatan, Özgür
name: Zorlu, Yunus
Publisher: name: Taylor & Francis
Year: 2020
Subjects: subject: Biophysics
subject: Biochemistry
subject: Space Science
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Cancer

description: A novel ternary copper(II) complexes, - [Cu(py-phen)(asn)(NO<sub>3</sub>)(H<sub>2</sub>O)] (<b>1</b>) and [Cu(py-phen)(trp)(H<sub>2</sub>O)]NO<sub>3</sub> (<b>2</b>)- (py-phen: pyrazino[2,3‐f][1,10]phenanthroline, asn: asparagine, trp: tryptophan), have been synthesized and characterized by CHN analysis, ESI-MS, FTIR and single-crystal X-ray diffraction techniques. Interaction of the complexes <b>1</b> and <b>2</b> with CT-DNA has been investigated by absorption spectral titration, EB and Hoechst 33258 displacement assay. The interaction between the complexes <b>1</b> and <b>2</b> and BSA was investigated by electronic absorption and fluorescence spectroscopy methods. The experimental outcomes indicate that the fluorescence quenching mechanism between the complexes <b>1</b> and <b>2</b> and BSA is a static quenching process. The Stern-Volmer constants, binding constants, binding sites and the corresponding thermodynamic parameters (ΔG, ΔH, ΔS) of BSA + complex systems were determined at different temperatures. The binding distance between the complexes <b>1</b> and <b>2</b> and BSA was calculated according to FRET. The effect of the complexes <b>1</b> and <b>2</b> on the conformation of BSA was also examined using synchronous, two dimensional (2D) and three dimensional (3D) fluorescence spectroscopy. Radical scavenging activity of the complex was determined in terms of EC<sub>50</sub>, using the DPPH and H<sub>2</sub>O<sub>2</sub> method. The anticancer activities of the complexes <b>1</b> and <b>2</b> were investigated using an XTT assay against three cancer cell lines (MCF-7, Caco-2 and A549) and non-tumor cell line (BEAS-2B). Communicated by Ramaswamy H. Sarma A potent drug candidature of two new copper(II) complexes, - [Cu(py-phen)(asn)(NO<sub>3</sub>)(H<sub>2</sub>O)] (<b>1</b>) and [Cu(py-phen)(trp)(H<sub>2</sub>O)]NO<sub>3</sub> (<b>2</b>)- (py-phen: pyrazino[2,3‐f][1,10]phenanthroline, asn: asparagine, trp: tryptophan), have been synthesized and characterized by CHN analysis, FTIR, ESI-MS and single-crystal X-ray diffraction techniques. The complexes have been tested for their <i>in vitro</i> biomolecular interactions by the spectroscopic methods. Furthermore, radical scavenging and anticancer activities of the complexes was also investigated. HighlightsA potent drug candidature of two new copper(II) complexes were synthesized and structurally characterized.Single crystal analysis of the two complexes was performed.The complexes interact with CT-DNA by a moderate intercalation binding mode.The quenching mechanism found between the complexes and BSA is a static type.The complexes demonstrate radical scavenging activity DPPH and H2O2 methods.Furthermore, the complexes exhibit anticancer activity against MCF-7, Caco-2 and A549 cell lines A potent drug candidature of two new copper(II) complexes were synthesized and structurally characterized. Single crystal analysis of the two complexes was performed. The complexes interact with CT-DNA by a moderate intercalation binding mode. The quenching mechanism found between the complexes and BSA is a static type. The complexes demonstrate radical scavenging activity DPPH and H2O2 methods. Furthermore, the complexes exhibit anticancer activity against MCF-7, Caco-2 and A549 cell lines