title: ÐÐµÐ·Ð¸ÐºÑÐ»Ñ ÑÐ°ÑÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ Ð¿ÑÐ¾Ð¸ÑÑ Ð¾Ð¶Ð´ÐµÐ½Ð¸Ñ ÐºÐ°Ðº Ð¿ÐµÑÐµÐ½Ð¾ÑÑÐ¸ÐºÐ¸ Ð±Ð¸Ð¾Ð°ÐºÑÐ¸Ð²Ð½ÑÑ Ð¼Ð¾Ð»ÐµÐºÑÐ»
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Plant vesicles as carriers of bioactive molecules
Creators: name: ÐÑÑÐµÐ²Ð¸Ñ, ÐÐ»ÐµÐ½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÑÐºÑÑÑÐ°ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐµ Ð²ÐµÐ·Ð¸ÐºÑÐ»Ñ ÑÐ°ÑÑÐ¸ÑÐµÐ»ÑÐ½ÑÐ³Ð¾ Ð¿ÑÐ¾Ð¸ÑÑ Ð¾Ð¶Ð´ÐµÐ½Ð¸Ñ
ÑÐºÐ·Ð¾Ð³ÐµÐ½Ð½ÑÐµ Ð±Ð¸Ð¾Ð¼Ð¾Ð»ÐµÐºÑÐ»Ñ
Ð²ÑÑÑÐ¸Ðµ ÑÐ°ÑÑÐµÐ½Ð¸Ñ
Ð¾Ð´Ð½Ð¾ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐµ Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ð¸
plant extracellular vesicles
exogenous biomolecules
higher plants
unicellular algae

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«ÐÐµÐ·Ð¸ÐºÑÐ»Ñ ÑÐ°ÑÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ Ð¿ÑÐ¾Ð¸ÑÑÐ¾Ð¶Ð´ÐµÐ½Ð¸Ñ ÐºÐ°Ðº Ð¿ÐµÑÐµÐ½Ð¾ÑÑÐ¸ÐºÐ¸ Ð±Ð¸Ð¾Ð°ÐºÑÐ¸Ð²Ð½ÑÑ Ð¼Ð¾Ð»ÐµÐºÑÐ»Â». ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° ÑÐ°ÑÑÐ¸ÑÐµÐ»ÑÐ½ÑÐ¼ ÑÐºÑÑÑÐ°ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐ¼ Ð²ÐµÐ·Ð¸ÐºÑÐ»Ð°Ð¼ (Ð Ð­Ð). Ð¦ÐµÐ»ÑÑ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ ÑÑÐ°Ð» Ð¿Ð¾Ð¸ÑÐº ÑÑÐµÐ´Ð¸ Ð Ð­Ð Ð¿ÐµÑÑÐ¿ÐµÐºÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ Ð´Ð¾ÑÑÐ°Ð²ÑÐ¸ÐºÐ° ÑÐºÐ·Ð¾Ð³ÐµÐ½Ð½ÑÑ Ð¼Ð¾Ð»ÐµÐºÑÐ» Ðº ÐºÐ»ÐµÑÐºÐ°Ð¼ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° in vitro. ÐÑÐ´ÐµÐ»ÐµÐ½Ð½ÑÐµ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ ÑÐ»ÑÑÑÐ°ÑÐµÐ½ÑÑÐ¸ÑÑÐ³Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¸Ð· ÑÐ¾ÐºÐ° Ð¿Ð»Ð¾Ð´Ð¾Ð² 7-Ð¼Ð¸ Ð²ÑÑÑÐ¸Ñ ÑÐ°ÑÑÐµÐ½Ð¸Ð¹ Ð¸ ÐºÑÐ»ÑÑÑÑÐ°Ð»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ´Ñ 2-Ñ ÑÐ¸Ð¿Ð¾Ð² Ð¾Ð´Ð½Ð¾ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹ Ð Ð­Ð Ð±ÑÐ»Ð¸ Ð¸Ð·ÑÑÐµÐ½Ñ Ð¼ÐµÑÐ¾Ð´Ð°Ð¼Ð¸ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° ÑÑÐ°ÐµÐºÑÐ¾ÑÐ¸Ð¸ Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸Ñ (nanoparticle tracking analysis - NTA), Ð°ÑÐ¾Ð¼Ð½Ð¾-ÑÐ¸Ð»Ð¾Ð²Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸ (AFM), ÐºÑÐ¸Ð¾ÑÐ»ÐµÐºÑÑÐ¾Ð½Ð½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸ (cryogenic electron microscopy - Cryo-EM), ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐ°ÑÑÐµÑÐ½Ð¸Ñ ÑÐ²ÐµÑÐ°. ÐÑÐ¸ Ð¿Ð¾Ð¼Ð¾ÑÐ¸ ÑÑÐ¸Ñ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð±ÑÐ»Ð¸ Ð¾ÑÐµÐ½ÐµÐ½Ñ ÑÐ°Ð·Ð¼ÐµÑÑ, ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ñ, Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ñ Ð¸ Ð´Ð·ÐµÑÐ°-Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð» ÑÐ°ÑÑÐ¸Ñ. Ð­ÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð½Ð°Ð³ÑÑÐ·ÐºÐ¸ Ð Ð­Ð ÑÐºÐ·Ð¾Ð³ÐµÐ½Ð½ÑÐ¼ Ð±ÐµÐ»ÐºÐ¾Ð¼ Ð±ÑÐ»Ð° Ð¾ÑÐµÐ½ÐµÐ½Ð° Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ ÑÐ»ÑÐ¾ÑÐ¸Ð¼ÐµÑÑÐ¸Ð¸, Ð° ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð´Ð¾ÑÑÐ°Ð²ÐºÐ¸ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾ Ð¼ÐµÑÐµÐ½Ð½Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° HSP70-Alexa Ð¿ÑÐ¸ Ð¿Ð¾Ð¼Ð¾ÑÐ¸ Ð Ð­Ð Ð² ÐºÐ»ÐµÑÐºÐ¸ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° in vitro Ð¾ÑÐµÐ½Ð¸Ð²Ð°Ð»Ð°ÑÑ Ð¿ÑÐ¸ Ð¿Ð¾Ð¼Ð¾ÑÐ¸ Ð¿ÑÐ¾ÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐ¸ÑÐ¾Ð¼ÐµÑÑÐ¸Ð¸. ÐÐ¾ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÐ¼ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ°Ð¼ Ð±ÑÐ» Ð¿ÑÐ¾Ð¸Ð·Ð²ÐµÐ´ÐµÐ½ ÑÑÐ°Ð²Ð½Ð¸ÑÐµÐ»ÑÐ½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð· Ð²ÑÐµÑ Ð Ð­Ð Ð¿Ð¾ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð½Ð°Ð³ÑÑÐ·ÐºÐ¸ Ð¸ Ð´Ð¾ÑÑÐ°Ð²ÐºÐ¸ ÑÐºÐ·Ð¾Ð³ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ°. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ ÑÐ°Ð±Ð¾ÑÑ, Ð±ÑÐ»Ð¸ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ñ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ Ð¿ÐµÑÑÐ¿ÐµÐºÑÐ¸Ð²Ð½ÑÐµ Ð² Ð¸Ð½Ð´ÑÑÑÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð¼ ÐºÐ»ÑÑÐµ Ð¿ÑÐ¾Ð´ÑÑÐµÐ½ÑÑ Ð Ð­Ð Ð¿Ð¾ ÑÐ¾Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ñ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð´Ð¾ÑÑÐ°Ð²ÐºÐ¸/ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð²ÑÑÐ¾Ð´Ð°.
description: The subject of the graduate qualification work is âPlant vesicles as carriers of bioactive moleculesâ. The given work is devoted to plant extracellular vesicles (PEVs). The aim of this work was to search among PEVs for a promising supplier of exogenous molecules to human cells in vitro. PEVs was isolated by ultracentrifugation from the fruit juice of 7 higher plants and the culture medium of 2 types of unicellular algae. PEVs were studied by nanoparticle tracking analysis (NTA), atomic force microscopy (AFM), cryogenic electron microscopy (Cryo-EM), electrophoretic light scattering. These methods were used to evaluate particle size, concentration, morphology, and zeta potential. The efficiency of loading PEVs with an exogenous protein was assessed by fluorimetry, and the efficiency of delivery of the fluorescently labeled HSP70-Alexa protein by PEVs to human cells in vitro was evaluated using flow cytometry. Based on the results obtained, a comparative analysis of all PEVs was carried out in terms of the efficiency of loading and delivery of exogenous protein. Thus, PEVs producers were identified, which in the future will be used as delivery systems for bioactive molecule.