cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
An excess of dusty starbursts related to the Spiderweb galaxy
Creators: name: Dannerbauer, H.
name: Kurk, J. D.
name: De Breuck, C.
name: Wylezalek, D.
name: Santos, J. S.
name: Koyama, Y.
name: Seymour, N.
name: Tanaka, M.
name: Hatch, N.
name: Altieri, B.
name: Coia, D.
name: Galametz, A.
name: Kodama, T.
name: Miley, G.
name: Röttgering, H.
name: Sanchez-Portal, M.
name: Valtchanov, I.
name: Venemans, B.
name: Ziegler, B.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Astrophysics of Galaxies (astro-ph.GA)
subject: FOS: Physical sciences

description: We present APEX LABOCA 870 micron observations of the field around the high-redshift radio galaxy MRC1138-262 at z=2.16. We detect 16 submillimeter galaxies in this ~140 square arcmin bolometer map with flux densities in the range 3-11 mJy. The raw number counts indicate a density of submillimeter galaxies (SMGs) that is up to four times that of blank field surveys. Based on an exquisite multiwavelength database, including VLA 1.4 GHz radio and infrared observations, we investigate whether these sources are members of the protocluster structure at z=2.2. Using Herschel PACS+SPIRE and Spitzer MIPS photometry, we derive reliable far-infrared photometric redshifts for all sources. Follow-up VLT ISAAC and SINFONI near-infrared spectra confirm that four of these SMGs have redshifts of z=2.2. We also present evidence that another SMG in this field, detected earlier at 850 micron, has a counterpart that exhibits Halpha and CO(1-0) emission at z=2.15. Including the radio galaxy and two SMGs with far-IR photometric redshifts at z=2.2, we conclude that at least eight submm sources are part of the protocluster at z=2.16 associated with the radio galaxy MRC1138-262. We measure a star formation rate density SFRD ~1500 Msun yr^-1 Mpc^-3, four magnitudes higher than the global SFRD of blank fields at this redshift. Strikingly, these eight sources are concentrated within a region of 2 Mpc (the typical size of clusters in the local universe) and are distributed within the filaments traced by the Halpha emitters at z=2.2. This concentration of massive, dusty starbursts is not centered on the submillimeter-bright radio galaxy which could support the infalling of these sources into the cluster center. Approximately half (6/11) of the SMGs that are covered by the Halpha imaging data are associated with Halpha emitters, demonstrating the potential of tracing SMG counterparts with this population (abridged).
description: Accepted by A&A. ESO Press Release on 15 October 2014. 19 pages, 9 figures and 4 tables
Functional dissection of adenovirus VAI RNA.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1989

description: During the course of adenovirus infection, the VAI RNA protects the translation apparatus of host cells by preventing the activation of host double-stranded RNA-activated protein kinase, which phosphorylates and thereby inactivates the protein synthesis initiation factor eIF-2. In the absence of VAI RNA, protein synthesis is drastically inhibited at late times in infected cells. The experimentally derived secondary structure of VAI RNA consists of two extended base-paired regions, stems I and III, which are joined by a short base-paired region, stem II, at the center. Stems I and II are joined by a small loop, A, and stem III contains a hairpin loop, B. At the center of the molecule and at the 3' side, stems II and III are connected by a short stem-loop (stem IV and hairpin loop C). A fourth, minor loop, D, exists between stems II and IV. To determine sequences and domains critical for function within this VAI RNA structure, we have constructed adenovirus mutants with linker-scan substitution mutations in defined regions of the molecule. Cells infected with these mutants were analyzed for polypeptide synthesis, virus yield, and eIF-2 alpha kinase activity. Our results showed that disruption of base-paired regions in the distal parts of the longest stems, I and III, did not affect function, whereas mutations causing structural perturbations in the central part of the molecule containing stem II, the proximal part of stem III, and the central short stem-loop led to loss of function. Surprisingly, one substitution mutant, sub742, although dramatically perturbing the integrity of the structure of this central portion, showed a wild-type phenotype, suggesting that an RNA with an alternate secondary structure is functional. On the basis of sensitivity to single-strand-specific RNases, we can derive a novel secondary structure for the mutant RNA in which a portion of the sequences may fold to form a structure that resembles the central part of the wild-type molecule, which suggests that only the short stem-loop located in the center of the molecule and the adjoining base-paired regions may define the functional domain. These results also imply that only a portion of the VAI RNA structure may be recognized by the host factor(s).
Evidence of resource partitioning between fin and sei whales during the twentieth-century whaling period
Creators: name: Buss, Danielle L
name: Hearne, Ella
name: Loy, Rebecca HY
name: Manica, Andrea
name: O’Connell, Tamsin C
name: Jackson, Jennifer A
Publisher: name: Apollo - University of Cambridge Repository
Year: 2022
Subjects: Original Paper
Balaenoptera borealis
Balaenoptera physalus
Resource partitioning
Stable isotope analysis
Whaling; South Georgia
Baleen

description: AbstractInvestigating resource partitioning of marine predators is essential for understanding coexistence of sympatric species and the functional role they play in marine ecosystems. Baleen whales are a key component of sub-Antarctic ecosystems, foraging predominantly on zooplankton and small forage fish. During the twentieth century, baleen whales were unsustainably exploited across the Southern Ocean. Within the exclusive economic zone of South Georgia and the South Sandwich Islands (SGSSI EEZ) in the South Atlantic, approximately 98,000 fin whales (Balaenoptera physalus) and 16,000 sei whales (B.borealis) were harvested. Despite both species historically occurring in high numbers and feeding in sub-polar waters, little is known about the mechanisms of coexistence. Here, by measuring stable isotope ratios of carbon (δ13C) and nitrogen (δ15N) in archived baleen plates and analysing historic catch data, we investigate resource partitioning of fin and sei whale during the commercial whaling period. Temporal and spatial occupancy at SGSSI EEZ (inferred from whaling catches that occurred between 1904 and 1976), alongside historic stomach contents (from the literature), and δ13C and δ15N results (observed in this study), suggests that despite using a common prey resource there was limited overlap in isotopic niches between the two species, with sei whales using SGSSI waters later in the season and for a shorter period than fin whales. We hypothesise that the isotopic differences were most likely due to sei whales foraging at lower latitudes prior to arrival at SGSSI. Our data provide novel insight into how two sympatric whale species co-occurring at SGSSI during the commercial whaling period may have partitioned resources and provide a potential ecological baseline to assess changes in resource use in recovering whale populations.
An organ-on-chip model of pulmonary arterial hypertension identifies a BMPR2-SOX17-prostacyclin signalling axis.
Creators: name: Ainscough, Alexander J
name: Smith, Timothy J
name: Haensel, Maike
name: Rhodes, Christopher J
name: Fellows, Adam
name: Whitwell, Harry J
name: Vasilaki, Eleni
name: Gray, Kelly
name: Freeman, Adrian
name: Howard, Luke S
name: Wharton, John
name: Dunmore, Benjamin
name: Upton, Paul D
name: Wilkins, Martin R
name: Edel, Joshua B
name: Wojciak-Stothard, Beata
Publisher: name: Apollo - University of Cambridge Repository
Year: 2022
Subjects: Article
/692/420
/631/80
/631/1647
article

description: Pulmonary arterial hypertension (PAH) is an unmet clinical need. The lack of models of human disease is a key obstacle to drug development. We present a biomimetic model of pulmonary arterial endothelial-smooth muscle cell interactions in PAH, combining natural and induced bone morphogenetic protein receptor 2 (BMPR2) dysfunction with hypoxia to induce smooth muscle activation and proliferation, which is responsive to drug treatment. BMPR2- and oxygenation-specific changes in endothelial and smooth muscle gene expression, consistent with observations made in genomic and biochemical studies of PAH, enable insights into underlying disease pathways and mechanisms of drug response. The model captures key changes in the pulmonary endothelial phenotype that are essential for the induction of SMC remodelling, including a BMPR2-SOX17-prostacyclin signalling axis and offers an easily accessible approach for researchers to study pulmonary vascular remodelling and advance drug development in PAH.
Identification and some properties of a unique DNA polymerase from cells infected with human B-lymphotropic virus.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1989

description: A new DNA polymerase and DNase activity were identified from cells infected with human B-lymphotropic herpesvirus (HBLV). DNA polymerase associated with HBLV infection was similar in its sensitivity to inhibition by ppi analogs as other herpesvirus-specific DNA polymerases but was dissimilar in its inhibition by certain nucleoside triphosphates.
Aging of aqueous laponite dispersions in the presence of sodium polystyrene sulfonate
Creators: name: Savenko, V.
name: Bulavin, L.
name: Rawiso, M.
name: Lebovka, N.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Soft Condensed Matter (cond-mat.soft)
subject: FOS: Physical sciences

description: Aqueous suspensions of Laponite with discotic particles are well-studied and find a wide range of applications in industry. A new direction of their implementation is polymer composites that can exhibit improved physical properties.We have studied the aging of aqueous suspensions of Laponite and sodium polystyrene sulfonate (PSS-Na) and both their microscopic (small-angle X-ray scattering, SAXS) and macroscopic (small amplitude oscillatory shear (SAOS) rheometry) properties. The concentration of Laponite, $C_L$, was fixed at 2.5% wt and concentration of PSS-Na, $C_p$, was varied within 0-0.5% wt (0-24.2~mM). It is shown that the adding of PSS-Na significantly accelerates the aging.Nevertheless, the systems were stable against the sedimentation, and the flocculation didn't occur. Polyelectrolyte induced the appearance of large-scale fractal heterogeneities, which became more compact in the course of the aging. Polyelectrolyte induced the appearance of large-scale fractal heterogeneities, which became more compact in the course of the aging.
description: 6 pages, 7 figures, (www.ujp.bitp.kiev.ua)
MPEC 2024-R340 : 2024 RV21
Creators: name: Minor Planet Center Staff
Publisher: name: The Minor Planet Center
Year: 2024
Subjects: subject: Small Solar System bodies
valueUri: http://astrothesaurus.org/uat/1469
subject: 2024 RV21

description: The Minor Planet Electronic Circulars contain information on unusual minor planets, routine data on comets and natural satellites, and occasional editorial announcements.  They are published on behalf of Division F of the International Astronomical Union by the Minor Planet Center, Smithsonian Astrophysical Observatory, Cambridge, MA 02138, U.S.A.
First step towards a consensus strategy for multi-locus diagnostic testing of imprinting disorders.
Creators: name: Mackay, Deborah
name: Bliek, Jet
name: Kagami, Masayo
name: Tenorio-Castano, Jair
name: Pereda, Arrate
name: Brioude, Frédéric
name: Netchine, Irène
name: Papingi, Dzhoy
name: De Franco, Elisa
name: Lever, Margaret
name: Sillibourne, Julie
name: Lombardi, Paola
name: Gaston, Véronique
name: Tauber, Maithé
name: Diene, Gwenaelle
name: Bieth, Eric
name: Fernandez, Luis
name: Nevado, Julian
name: Tümer, Zeynep
name: Riccio, Andrea
name: Maher, Eamonn R
name: Beygo, Jasmin
name: Tannorella, Pierpaola
name: Russo, Silvia
name: De Nanclares, Guiomar Perez
name: Temple, I Karen
name: Ogata, Tsutomu
name: Lapunzina, Pablo
name: Eggermann, Thomas
Publisher: name: Apollo - University of Cambridge Repository
Year: 2022
Subjects: Research
Imprinting disorders
Genetic testing
Multi-locus testing
Unexpected molecular diagnosis
Overlapping phenotypes
Multi-locus imprinting disorder

description: Funder: RWTH Aachen University (3131)
description: BACKGROUND: Imprinting disorders, which affect growth, development, metabolism and neoplasia risk, are caused by genetic or epigenetic changes to genes that are expressed from only one parental allele. Disease may result from changes in coding sequences, copy number changes, uniparental disomy or imprinting defects. Some imprinting disorders are clinically heterogeneous, some are associated with more than one imprinted locus, and some patients have alterations affecting multiple loci. Most imprinting disorders are diagnosed by stepwise analysis of gene dosage and methylation of single loci, but some laboratories assay a panel of loci associated with different imprinting disorders. We looked into the experience of several laboratories using single-locus and/or multi-locus diagnostic testing to explore how different testing strategies affect diagnostic outcomes and whether multi-locus testing has the potential to increase the diagnostic efficiency or reveal unforeseen diagnoses. RESULTS: We collected data from 11 laboratories in seven countries, involving 16,364 individuals and eight imprinting disorders. Among the 4721 individuals tested for the growth restriction disorder Silver-Russell syndrome, 731 had changes on chromosomes 7 and 11 classically associated with the disorder, but 115 had unexpected diagnoses that involved atypical molecular changes, imprinted loci on chromosomes other than 7 or 11 or multi-locus imprinting disorder. In a similar way, the molecular changes detected in Beckwith-Wiedemann syndrome and other imprinting disorders depended on the testing strategies employed by the different laboratories. CONCLUSIONS: Based on our findings, we discuss how multi-locus testing might optimise diagnosis for patients with classical and less familiar clinical imprinting disorders. Additionally, our compiled data reflect the daily life experiences of diagnostic laboratories, with a lower diagnostic yield than in clinically well-characterised cohorts, and illustrate the need for systematising clinical and molecular data.
M protein (M1) of influenza virus: antigenic analysis and intracellular localization with monoclonal antibodies.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1989

description: A panel of 16 monoclonal antibodies recognizing M protein (M1) of influenza virus was generated. Competition analyses resulted in localization of 14 monoclonal antibodies to three antigenic sites. Antigenic variation of M proteins was seen when the monoclonal antibodies were tested against 14 strains of type A influenza viruses. Immunofluorescence analysis with the monoclonal antibodies showed migration of M protein to the nucleus during the replicative cycle and demonstrated association of M protein with actin filaments in the cytoplasm.A panel of 16 monoclonal antibodies recognizing M protein (M1) of influenza virus was generated. Competition analyses resulted in localization of 14 monoclonal antibodies to three antigenic sites. Antigenic variation of M proteins was seen when the monoclonal antibodies were tested against 14 strains of type A influenza viruses. Immunofluorescence analysis with the monoclonal antibodies showed migration of M protein to the nucleus during the replicative cycle and demonstrated association of M protein with actin filaments in the cytoplasm.
Mutational analysis of human immunodeficiency virus type 1 protease suggests functional homology with aspartic proteinases.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1989

description: Processing of the retroviral gag and pol gene products is mediated by a viral protease. Bacterial expression systems have been developed which permit genetic analysis of the human immunodeficiency virus type 1 protease as measured by cleavage of the pol protein precursor. Deletion analysis of the pol reading frame locates the sequences required to encode a protein with appropriate proteolytic activity near the left end of the pol reading frame but largely outside the gag-pol overlap region, which is at the extreme left end of pol. Most missense mutations within an 11-amino-acid domain highly conserved among retroviral proteases and with sequence similarity to the active site of aspartic proteinases abolish appropriate processing, suggesting that the retrovirus proteases share a catalytic mechanism with aspartic proteinases. Substitution of the amino acids flanking the scissile bond at three of the processing sites encoded by pol demonstrates distinct sequence requirements for cleavage at these different sites. The inclusion of a charged amino acid at the processing site blocks cleavage. A subset of these substitutions also inhibits processing at the nonmutated sites.
Interfacial Dzyaloshinskii-Moriya interaction in Ta\Co20Fe60B20\MgO nanowires
Creators: name: Conte, R. Lo
name: Martinez, E.
name: Hrabec, A.
name: Lamperti, A.
name: Schulz, T.
name: Nasi, L.
name: Lazzarini, L.
name: Maccherozzi, F.
name: Dhesi, S. S.
name: Ocker, B.
name: Marrows, C. H.
name: Moore, T. A.
name: Klaeui, M.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Mesoscale and Nanoscale Physics (cond-mat.mes-hall)
subject: FOS: Physical sciences

description: We report current-induced domain wall motion (CIDWM) in Ta\Co20Fe60B20\MgO nanowires. Domain walls are observed to move against the electron flow when no magnetic field is applied, while a field along the nanowires strongly affects the domain wall motion direction and velocity. A symmetric effect is observed for up-down and down-up domain walls. This indicates the presence of right-handed domain walls, due to a Dzyaloshinskii-Moriya interaction (DMI) with a DMI coefficient D=+0.06 mJ/m2. The positive DMI coefficient is interpreted to be a consequence of boron diffusion into the tantalum buffer layer during annealing. In a Pt\Co68Fe22B10\MgO nanowire CIDWM along the electron flow was observed, corroborating this interpretation. The experimental results are compared to 1D-model simulations including the effects of pinning. This advanced modelling allows us to reproduce the experiment outcomes and reliably extract a spin-Hall angle θSH=-0.11 for Ta in the nanowires, showing the importance of an analysis that goes beyond the currently used model for perfect nanowires.
description: 22 pages, 5 figures
Inclination Dependence of Lyman-Alpha Properties in a Turbulent Disk Galaxy
Creators: name: Behrens, Christoph
name: Braun, Harald
Publisher: name: arXiv
Year: 2014
Subjects: subject: Astrophysics of Galaxies (astro-ph.GA)
subject: FOS: Physical sciences

description: We present simulations of Lyman-Alpha radiation transfer in an isolated disk galaxy with a turbulence sub-grid model, multi-phase interstellar medium and detailed star formation modelling. We investigate the influence of inclination on the observed Lyman-Alpha properties for different snapshots. The Lyman-alpha spectrum, equivalent width distribution and escape fractions vary significantly with the detailed morphology of the disk, leading to variations from one snapshot to another. In particular, we find that supernova-driven cavities near star-forming regions in the simulation can dominate the transmitted Lyman-alpha fraction, suggesting a variability of LAEs on the timescales of the star formation activity.
description: accepted for publication, 9 pages
Exchange interaction between the triplet exciton and the localized spin in copper-phthalocyanine
Creators: name: Wu, Wei
Publisher: name: arXiv
Year: 2014
Subjects: subject: Quantum Physics (quant-ph)
subject: Materials Science (cond-mat.mtrl-sci)
subject: Atomic and Molecular Clusters (physics.atm-clus)
subject: FOS: Physical sciences

description: Triplet excitonic state in the organic molecule may arise from a singlet excitation and the following inter-system crossing. Especially for a spin-bearing molecule, an exchange interaction between the triplet exciton and the original spin on the molecule can be expected. In this paper, such exchange interaction in copper-phthalocyanine (CuPc, spin-$\frac{1}{2}$) was investigated from first-principles by using density-functional theory within a variety of approximations to the exchange correlation, ranging from local-density approximation to long-range corrected hybrid-exchange functional. The magnitude of the computed exchange interaction is in the order of meV with the minimum value (1.5 meV, ferromagnetic) given by the long-range corrected hybrid-exchange functional CAM-B3LYP. This exchange interaction can therefore give rise to a spin coherence with an oscillation period in the order of picoseconds, which is much shorter than the triplet lifetime in CuPc (typically tens of nanoseconds). This implies that it might be possible to manipulate the localised spin on Cu experimentally using optical excitation and inter-system crossing well before the triplet state disappears.
description: 6 pages, 3 figures, WW2014_TripletExchange
NAVIGATOR: an Italian regional imaging biobank to promote precision medicine for oncologic patients.
Creators: name: Borgheresi, Rita
name: Barucci, Andrea
name: Colantonio, Sara
name: Aghakhanyan, Gayane
name: Assante, Massimiliano
name: Bertelli, Elena
name: Carlini, Emanuele
name: Carpi, Roberto
name: Caudai, Claudia
name: Cavallero, Diletta
name: Cioni, Dania
name: Cirillo, Roberto
name: Colcelli, Valentina
name: Dell'Amico, Andrea
name: Di Gangi, Domnico
name: Erba, Paola Anna
name: Faggioni, Lorenzo
name: Falaschi, Zeno
name: Gabelloni, Michela
name: Gini, Rosa
name: Lelii, Lucio
name: Liò, Pietro
name: Lorito, Antonio
name: Lucarini, Silvia
name: Manghi, Paolo
name: Mangiacrapa, Francesco
name: Marzi, Chiara
name: Mazzei, Maria Antonietta
name: Mercatelli, Laura
name: Mirabile, Antonella
name: Mungai, Francesco
name: Miele, Vittorio
name: Olmastroni, Maristella
name: Pagano, Pasquale
name: Paiar, Fabiola
name: Panichi, Giancarlo
name: Pascali, Maria Antonietta
name: Pasquinelli, Filippo
name: Shortrede, Jorge Eduardo
name: Tumminello, Lorenzo
name: Volterrani, Luca
name: Neri, Emanuele
name: NAVIGATOR Consortium Group
Publisher: name: Apollo - University of Cambridge Repository
Year: 2022
Subjects: Guideline/Position paper
Artificial intelligence
Biobanks
Biomarkers
Precision medicine
Radiomics

description: NAVIGATOR is an Italian regional project boosting precision medicine in oncology with the aim of making it more predictive, preventive, and personalised by advancing translational research based on quantitative imaging and integrative omics analyses. The project's goal is to develop an open imaging biobank for the collection and preservation of a large amount of standardised imaging multimodal datasets, including computed tomography, magnetic resonance imaging, and positron emission tomography data, together with the corresponding patient-related and omics-related relevant information extracted from regional healthcare services using an adapted privacy-preserving model. The project is based on an open-source imaging biobank and an open-science oriented virtual research environment (VRE). Available integrative omics and multi-imaging data of three use cases (prostate cancer, rectal cancer, and gastric cancer) will be collected. All data confined in NAVIGATOR (i.e., standard and novel imaging biomarkers, non-imaging data, health agency data) will be used to create a digital patient model, to support the reliable prediction of the disease phenotype and risk stratification. The VRE that relies on a well-established infrastructure, called D4Science.org, will further provide a multiset infrastructure for processing the integrative omics data, extracting specific radiomic signatures, and for identification and testing of novel imaging biomarkers through big data analytics and artificial intelligence.
Towards Chemical Constraints on Hot Jupiter Migration
Creators: name: Madhusudhan, Nikku
name: Amin, Mustafa A.
name: Kennedy, Grant M.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Earth and Planetary Astrophysics (astro-ph.EP)
subject: FOS: Physical sciences

description: The origin of hot Jupiters -- gas giant exoplanets orbiting very close to their host stars -- is a long-standing puzzle. Planet formation theories suggest that such planets are unlikely to have formed in-situ but instead may have formed at large orbital separations beyond the snow line and migrated inward to their present orbits. Two competing hypotheses suggest that the planets migrated either through interaction with the protoplanetary disk during their formation, or by disk-free mechanisms such as gravitational interactions with a third body. Observations of eccentricities and spin-orbit misalignments of hot Jupiter systems have been unable to differentiate between the two hypotheses. In the present work, we suggest that chemical depletions in hot Jupiter atmospheres might be able to constrain their migration mechanisms. We find that sub-solar carbon and oxygen abundances in Jovian-mass hot Jupiters around Sun-like stars are hard to explain by disk migration. Instead, such abundances are more readily explained by giant planets forming at large orbital separations, either by core accretion or gravitational instability, and migrating to close-in orbits via disk-free mechanisms involving dynamical encounters. Such planets also contain solar or super-solar C/O ratios. On the contrary, hot Jupiters with super-solar O and C abundances can be explained by a variety of formation-migration pathways which, however, lead to solar or sub-solar C/O ratios. Current estimates of low oxygen abundances in hot Jupiter atmospheres may be indicative of disk-free migration mechanisms. We discuss open questions in this area which future studies will need to investigate.
description: Accepted for publication in ApJ Letters
The Epstein-Barr virus (EBV) BZLF1 immediate-early gene product differentially affects latent versus productive EBV promoters.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1989

description: The Epstein-Barr virus (EBV) BZLF1 gene product is thought to mediate the disruption of latent EBV infection. We have examined the regulatory effects of BZLF1 by studying its transactivating effects on seven different EBV promoters. We find that whereas the BZLF1 gene product increases the activity of the two early promoters, BMLF1 and BMRF1, it decreases the activity of three latent promoters (the BamHI-C and BamHI-W Epstein-Barr nuclear antigen promoters and the latent membrane protein promoter). The BZLF1-induced changes in promoter-directed chloramphenicol acetyltransferase activity occur in EBV-negative as well as EBV-positive cell lines and are accompanied by a similar change in chloramphenicol acetyltransferase mRNA. Deletion analysis of the BamHI Z fragment indicates that in a portion of the amino-terminal half of the BZLF1 gene product (amino acids 24 to 86) is not essential for positive transactivating effects but is required for down-regulating effects. Thus, different domains of the same EBV immediate-early gene product can either increase the function of EBV promoters active in productive infection or decrease the function of key promoters active in latent infection.
Towards a Practical Architecture for India Centric Internet of Things
Creators: name: Misra, Prasant
name: Simmhan, Yogesh
name: Warrior, Jay
Publisher: name: arXiv
Year: 2014
Subjects: subject: Human-Computer Interaction (cs.HC)
subject: Computers and Society (cs.CY)
subject: FOS: Computer and information sciences

description: An effective architecture for the Internet of Things (IoT), particularly for an emerging nation like India with limited technology penetration at the national scale, should be based on tangible technology advances in the present, practical application scenarios of social and entrepreneurial value, and ubiquitous capabilities that make the realization of IoT affordable and sustainable. Humans, data, communication and devices play key roles in the IoT ecosystem that we perceive. In a push towards this sustainable and practical IoT Architecture for India, we synthesize ten design paradigms to consider.
The Epstein-Barr virus BMLF1 promoter contains an enhancer element that is responsive to the BZLF1 and BRLF1 transactivators.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1989

description: Here, we show that the EBV BRLF1 immediate-early gene product transactivates the BMLF1 promoter in either EBV-positive or EBV-negative B cells. Deletional analysis revealed that both the BZLF1-responsive region and the BRLF1-responsive region of the BMLF1 promoter are contained within the same 140-base-pair FokI-PvuII fragment located 300 base pairs upstream of the mRNA start site. The effect of BZLF1 on the BMLF1 promoter is likely to be indirect and mediated through the BRLF1 transactivator.Here, we show that the EBV BRLF1 immediate-early gene product transactivates the BMLF1 promoter in either EBV-positive or EBV-negative B cells. Deletional analysis revealed that both the BZLF1-responsive region and the BRLF1-responsive region of the BMLF1 promoter are contained within the same 140-base-pair FokI-PvuII fragment located 300 base pairs upstream of the mRNA start site. The effect of BZLF1 on the BMLF1 promoter is likely to be indirect and mediated through the BRLF1 transactivator.
The Epstein-Barr virus immediate-early gene product, BMLF1, acts in trans by a posttranscriptional mechanism which is reporter gene dependent.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1989

description: In DNA cotransfection experiments, the Epstein-Barr virus immediate-early gene product, BMLF1, stimulated the chloramphenicol acetyltransferase (CAT) activity of both latent and productive EBV promoters linked to CAT. This BMLF1-induced increase in CAT activity was out of proportion to the effect on CAT mRNA, suggesting a posttranscriptional mechanism. Furthermore, when growth hormone was used as a reporter gene instead of CAT, BMLF1 no longer functioned. Thus, the BMLF1 effect was reporter-gene dependent. The effect of the BMLF1 gene product does not then appear to be directed at promoter activation, but instead may function to increase the level of an as yet unidentified protein(s) required for Epstein-Barr virus infection.
Monoclonal B-cell lymphocytosis
Creators: name: D'Arena, Giovanni
name: Musto, Pellegrino
Publisher: name: Universita' degli Studi di Salerno
Year: 2014
Subjects: Monoclonal B-cell lymphocytosis
Chronic lymphocytic leukemia
Diagnostic criteria
Management

description: Monoclonal B-cell lymphocytosis (MBL) is an asymptomatic hematologic condition defined by the presence of a small (<5 x 109/L) clonal B-cell population in the peripheral blood in the absence of lymph-node enlargement, cytopenias or autoimmune diseases. It is found in approximately 3-12% of normal persons depending on the accuracy of analytical techniques applied. According to the immunophenotypic profile of clonal B-cells, the majority of MBL cases (75%) are classified as chronic lymphocytic leukemia (CLL)-like. This form may progress into CLL at a rate of 1–2% per year. It is thought that CLL is always preceded by MBL. The remaining MBL cases are defined as atypical CLL-like (CD5+/CD20bright) and CD5- MBL. The MBL clone size is quite heterogenous. Accordingly, two forms of MBL are identified: i) high-count, or ‘clinical’ MBL, in which an evidence of lymphocytosis (<5 x 109/L clonal B-cells) is seen, and ii) a low-count MBL, in which a normal leukocyte count is found and that is identified only in population-screening studies. Both forms of MBL may carry the cytogenetic abnormalities that are the hallmark of CLL, including 13q-, 17p- and trisomy 12. Consistent with the indolent phenotype of this condition, genetic lesions, such as TP53, ATM, NOTCH1 and SF3B1 mutations, usually associated with high-risk CLL, are rarely seen. Overall, no prognostic indicator of evolution of MBL to overt CLL has been found at present time. However, taking into account this possibility, a clinical and lab monitoring (at least annually), is recommended.
Concatameric replication of Epstein-Barr virus: structure of the termini in virus-producer and newly transformed cell lines.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1990

description: The linear form of Epstein-Barr virus (EBV) DNA has homologous direct tandem repeats of approximately 500 bp at each terminus (TR). After infection, EBV DNA circularizes via the TR to form the intracellular episomal DNA. To analyze the mechanism of the synthesis of linear DNA through possible replicative intermediates, the terminal fragments were identified in the total intracellular DNA and the covalently closed circular DNA from a productively infected cell line after induction of replication or after treatment with an inhibitor of viral DNA synthesis. These studies indicate that some of the fused terminal fragments detected in the total intracellular DNA are replication-dependent forms which are selectively excluded from the covalently closed circular fraction and are eliminated after treatment with acyclovir. The EBV terminal restriction enzyme fragments were identified in three producer cell lines, each with a characteristic number of TR in the intracellular episomal DNA. Identification of the termini in cell lines established with the three virus strains revealed that the newly transformed cell lines had a greater number of TR than did the template DNA in the producer cell line. The increase in the number of TR in progeny episomes indicates that linear DNA is produced from concatameric replicative intermediates rather than from amplified catenated circular intermediates.
Defective viral DNA in Epstein-Barr virus-associated oral hairy leukoplakia.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1990

description: Defective Epstein-Barr virus (EBV) has a deleted and rearranged genome (termed het DNA) that disrupts latency and induces standard EBV to replicate in vitro. We used the polymerase chain reaction to detect, in 2 of 10 patient samples, the junction of abnormally juxtaposed EBV DNA fragments BamHI W and Z, a genomic rearrangement responsible for the biologic activity of het DNA. By sequence analysis, the junction in wild-type defective DNA appears to be similar but not identical to the recombination in the DNA of laboratory strain P3HR-1. The presence of this marker for het DNA in the epithelial lesions of two patients suggests a role for defective EBV in a human pathologic process.
Immunogenic domains of hepatitis delta virus antigen: peptide mapping of epitopes recognized by human and woodchuck antibodies.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1990

description: Hepatitis delta virus (HDV) is a defective RNA virus which is dependent on hepatitis B virus for essential helper functions. Only a single highly basic phosphoprotein, HDV antigen (HDAg), is expressed by the HDV genome during infection in humans. Antibody directed to HDAg is important in the diagnosis of HDV infection, and it is likely but not yet proven that the immune response to HDAg provides significant protection against subsequent exposures to HDV. In an effort to map the antigenic domains of HDAg, 209 overlapping hexapeptides, spanning the entire 214 amino acid residues of the protein, were synthesized on polyethylene pins and probed by enzyme-linked immunosorbent assay with sera containing high titers of anti-HD antibodies. Domains recognized by antibodies present in serum from human chronic carriers of this virus included residues 2 to 7, 63 to 74, 86 to 91, 94 to 100, 159 to 172, 174 to 195, and 197 to 207. Antibody from an acutely superinfected woodchuck recognized similar epitopes, as well as a domain spanning residues 121 to 128. Together, residues in these antigenic domains constitute 41% of the HDAg molecule. Oligopeptides 15 to 29 residues in length and representing epitopes of HDAg found to be dominant in humans (residues 2 to 17, 156 to 184, and 197 to 211) were synthesized in bulk and found to possess significant antigenic activity by microdilution enzyme-linked immunosorbent assay. The reactivity of peptide 197-211 with human sera confirms that the entire 214 amino acids of HDAg are expressed during infection in vivo. In addition, these results suggest that synthetic peptides may be useful reagents for development of new and improved diagnostic tests for HDV infection.
Novel transcription from the Epstein-Barr virus terminal EcoRI fragment, DIJhet, in a nasopharyngeal carcinoma.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1990

description: The data suggest that the C15 tumor represents a latently infected tumor and that the transcription from BamHI-A, which is expressed in all cells, is not associated with virus replication.The data suggest that the C15 tumor represents a latently infected tumor and that the transcription from BamHI-A, which is expressed in all cells, is not associated with virus replication.
Supplementary material for "Characterisation of oxylipin receptors and their potential role in inter-partner signalling in a model cnidarian-dinoflagellate symbiosis"
Creators: name: Gamba, Andrea G.
name: Oakley, Clinton A.
name: Ashley, Immy A,
name: Grossman, Arthur R.
name: Weis, Virginia M.
name: Suggett, David J.
name: Davy, Simon K.
Publisher: name: Zenodo
Year: 2024

description: Supplementary material of "Characterisation of oxylipin receptors and their potential role in inter-partner signalling in a model cnidarian-dinoflagellate symbiosis"
Analysis of the spiral structure in a simulated galaxy
Creators: name: Mata-Chávez, Dolores
name: Gómez, Gilberto C.
name: Puerari, Ivânio
Publisher: name: arXiv
Year: 2014
Subjects: subject: Astrophysics of Galaxies (astro-ph.GA)
subject: FOS: Physical sciences

description: We analyze the spiral structure that results in a numerical simulation of a galactic disk with stellar and gaseous components evolving in a potential that includes an axisymmetric halo and bulge. We perform a second simulation without the gas component to observe how it affects the spiral structure in the disk. To quantify this, we use a Fourier analysis and obtain values for the pitch angle and the velocity of the self-excited spiral pattern of the disk. The results show a tighter spiral in the simulation with gaseous component. The spiral structure is consistent with a superposition of waves, each with a constant pattern velocity in given radial ranges.
Experimental realization of a dynamic squeezing gate
Creators: name: Miyata, Kazunori
name: Ogawa, Hisashi
name: Marek, Petr
name: Filip, Radim
name: Yonezawa, Hidehiro
name: Yoshikawa, Jun-ichi
name: Furusawa, Akira
Publisher: name: arXiv
Year: 2014
Subjects: subject: Quantum Physics (quant-ph)
subject: FOS: Physical sciences

description: Squeezing is a nonlinear Gaussian operation that is the key component in construction of other nonlinear Gaussian gates. In our implementation of the squeezing gate, the amount and the orientation of the squeezing can be controlled by an external driving signal with 1 MHz operational bandwidth. This opens a brand new area of dynamic Gaussian processing. In particular, the gate can be immediately employed as the feed-forward needed for the deterministic implementation of the quantum cubic gate, which is a key piece of universal quantum information processing.
description: 7 pages, 5 figures
Enabling Portability and Reusability of Open Science Infrastructures
Creators: name: Heibi, Ivan
Publisher: name: Zenodo
Year: 2022
Subjects: Open Science Infrastructures
OpenCitations
FAIR
POSI

description: The slides used in the presentation held by Ivan Heibi in the context of the 26th International Conference on Theory and Practice of Digital Libraries (TPDL 2022). Abstract This paper presents a methodology for designing a containerized and distributed open science infrastructure to simplify its reusability, replicability, and portability in different environments. The methodology is depicted in a step-by-step schema based on four main phases: (1) Analysis, (2) Design, (3) Definition, and (4) Managing and provisioning. We accompany the description of each step with existing technologies and concrete examples of application.
Higgs production in $e^{-}e^{+}$ collisions as a probe of noncommutativity
Creators: name: Ghasemkhani, M.
name: Goldouzian, R.
name: Khanpour, H.
name: Khatiri, M.
name: Mohammadi, M.
Publisher: name: arXiv
Year: 2014
Subjects: subject: High Energy Physics - Phenomenology (hep-ph)
subject: FOS: Physical sciences

description: We examine the sensitivity of the angular distribution of the Higgs boson in the process of $e^+e^-\to Z H$ and the total cross section in the minimal noncommutative standard model (mNCSM) framework to set lower limit on the noncommutative charactristic scale ($Λ$). Contrary to the standard model case, in this process the Higgs boson tends to be emitted anisotropically in the transverse plane. Based on this fact, the profile likelihood ratio is used to set lower limit on $Λ$. The lower limit is presented as a function of the integrated luminosity. We show that at the center-of-mass energy of 1.5 TeV and with 500 fb$^{-1}$ of data, the noncommutative characteristic energy scale $Λ$ can be excluded up to 1.2 TeV.
description: 6 figures, 2 tables
Plasmid origin of replication of herpesvirus papio: DNA sequence and enhancer function.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1990

description: Herpesvirus papio (HVP) is a lymphotropic virus of baboons which is related to Epstein-Barr virus (EBV) and produces latent infection. The nucleotide sequence of the 5,775-base-pair (bp) EcoRI K fragment of HVP, which has previously been shown to confer the ability to replicate autonomously, has been determined. Within this DNA fragment is a region which bears structural and sequence similarity to the ori-P region of EBV. The HVP ori-P region has a 10- by 26-bp tandem array which is related to the 20- by 30-bp tandem array from the EBV ori-P region. In HVP there is an intervening region of 764 bp followed by five partial copies of the 26-bp monomer. Both the EBV and HVP 3' regions have the potential to form dyad structures which, however, differ in arrangement. We also demonstrate that a transcriptional enhancer which requires transactivation by a virus-encoded factor is present in the HVP ori-P.
Fixed-Node Diffusion Monte Carlo potential energy curve of the fluorine molecule F2 using selected configuration interaction trial wavefunctions
Creators: name: Giner, Emmanuel
name: Scemama, Anthony
name: Caffarel, Michel
Publisher: name: arXiv
Year: 2014
Subjects: subject: Chemical Physics (physics.chem-ph)
subject: FOS: Physical sciences

description: The potential energy curve of the F$_2$ molecule is calculated with Fixed-Node Diffusion Monte Carlo (FN-DMC) using Configuration Interaction (CI)-type trial wavefunctions. To keep the number of determinants reasonable (the first and second derivatives of the trial wavefunction need to be calculated at each step of FN-DMC), the CI expansion is restricted to those determinants that contribute the most to the total energy. The selection of the determinants is made using the so-called CIPSI approach (Configuration Interaction using a Perturbative Selection made Iteratively). Quite remarkably, the nodes of CIPSI wavefunctions are found to be systematically improved when increasing the number of selected determinants. To reduce the non-parallelism error of the potential energy curve a scheme based on the use of a $R$-dependent number of determinants is introduced. Numerical results show that improved FN-DMC energy curves for the F$_2$ molecule are obtained when employing CIPSI trial wavefunctions. Using the Dunning's cc-pVDZ basis set the FN-DMC energy curve is of a quality similar to that obtained with FCI/cc-pVQZ. A key advantage of using selected CI in FN-DMC is the possibility of improving nodes in a systematic and automatic way without resorting to a preliminary multi-parameter stochastic optimization of the trial wavefunction performed at the Variational Monte Carlo level as usually done in FN-DMC.
description: 16 pages, 15 figures
Rearrangements in unintegrated retroviral DNA are complex and are the result of multiple genetic determinants.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1990

description: We used a replication-competent retrovirus shuttle vector based on a DNA clone of the Schmidt-Ruppin A strain of Rous sarcoma virus to characterize rearrangements in circular viral DNA. In this system, circular molecules of viral DNA present after acute infection of cultured cells were cloned as plasmids directly into bacteria. The use of a replication-competent shuttle vector permitted convenient isolation of a large number of viral DNA clones; in this study, over 1,000 clones were analyzed. The circular DNA molecules could be placed into a limited number of categories. Approximately one-third of the rescued molecules had deletions in which one boundary was very near the edge of a long terminal repeat (LTR) unit. Subtle differences in the patterns of deletions in circular DNAs with one versus two copies of the LTR sequence were observed, and differences between deletions emanating from the right and left boundaries of the LTR were seen. A virus with a missense mutation in the region of the pol gene responsible for integration and exhibiting a temperature sensitivity phenotype for replication had a marked decrease in the number of rescued molecules with LTR-associated deletions when infection was performed at the nonpermissive temperature. This result suggests that determinants in the pol gene, possibly in the integration protein, play a role in the generation of LTR-associated deletions. Sequences in a second region of the genome, probably within the viral gag gene, were also found to affect the types of circular viral DNA molecules present after infection. Sequences in this region from different strains of avian sarcoma-leukosis viruses influenced the fraction of circular molecules with LTR-associated deletions, as well as the relative proportion of circular molecules with either one or two copies of the LTR. Thus, the profile of rearrangements in unintegrated viral DNA is complex and dependent upon the nature of sequences in the gag and pol regions.