Drug susceptibility testing and mortality in patients treated for tuberculosis in high-burden countries: a multicentre cohort study
Creators: name: Zürcher, Kathrin
name: Ballif, Marie
name: Fenner, Lukas
name: Borrell, Sonia
name: Keller, Peter M
name: Gnokoro, Joachim
name: Marcy, Olivier
name: Yotebieng, Marcel
name: Lameck Diero
name: E Jane Carter
name: Neesha Rockwood
name: Wilkinson, Robert J
name: Cox, Helen
name: Ezati, Nicholas
name: Alash'le G Abimiku
name: Collantes, Jimena
name: Anchalee Avihingsanon
name: Kamon Kawkitinarong
name: Reinhard, Miriam
name: Hömke, Rico
name: Huebner, Robin
name: Gagneux, Sebastien
name: Böttger, Erik C
name: Egger, Matthias
name: International Epidemiology Databases To Evaluate AIDS (IeDEA) Consortium
Publisher: name: The Francis Crick Institute
Year: 2019
Subjects: subject: Model organisms
subject: Infectious Disease
subject: Human Biology &amp; Physiology
subject: Immunology
subject: FOS: Clinical medicine

description: BACKGROUND: Drug resistance is a challenge for the global control of tuberculosis. We examined mortality in patients with tuberculosis from high-burden countries, according to concordance or discordance of results from drug susceptibility testing done locally and in a reference laboratory. METHODS: This multicentre cohort study was done in Côte d'Ivoire, Democratic Republic of the Congo, Kenya, Nigeria, South Africa, Peru, and Thailand. We collected Mycobacterium tuberculosis isolates and clinical data from adult patients aged 16 years or older. Patients were stratified by HIV status and tuberculosis drug resistance. Molecular or phenotypic drug susceptibility testing was done locally and at the Swiss National Center for Mycobacteria, Zurich, Switzerland. We examined mortality during treatment according to drug susceptibility test results and treatment adequacy in multivariable logistic regression models adjusting for sex, age, sputum microscopy, and HIV status. FINDINGS: We obtained M tuberculosis isolates from 871 patients diagnosed between 2013 and 2016. After exclusion of 237 patients, 634 patients with tuberculosis were included in this analysis; the median age was 33·2 years (IQR 26·9-42·5), 239 (38%) were women, 272 (43%) were HIV-positive, and 69 (11%) patients died. Based on the reference laboratory drug susceptibility test, 394 (62%) strains were pan-susceptible, 45 (7%) monoresistant, 163 (26%) multidrug-resistant (MDR), and 30 (5%) had pre-extensively or extensively drug resistant (pre-XDR or XDR) tuberculosis. Results of reference and local laboratories were concordant for 513 (81%) of 634 patients and discordant for 121 (19%) of 634. Overall, sensitivity to detect any resistance was 90·8% (95% CI 86·5-94·2) and specificity 84·3% (80·3-87·7). Mortality ranged from 6% (20 of 336) in patients with pan-susceptible tuberculosis treated according to WHO guidelines to 57% (eight of 14) in patients with resistant strains who were under-treated. In logistic regression models, compared with concordant drug susceptibility test results, the adjusted odds ratio of death was 7·33 (95% CI 2·70-19·95) for patients with discordant results potentially leading to under-treatment. INTERPRETATION: Inaccurate drug susceptibility testing by comparison with a reference standard leads to under-treatment of drug-resistant tuberculosis and increased mortality. Rapid molecular drug susceptibility test of first-line and second-line drugs at diagnosis is required to improve outcomes in patients with MDR tuberculosis and pre-XDR or XDR tuberculosis. FUNDING: National Institutes of Allergy and Infectious Diseases, Swiss National Science Foundation, Swiss National Center for Mycobacteria.