title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸Ñ ÑÐµÑÐµÐ±ÑÐ° Ð½Ð° Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¼ Ð¼ÐµÐ´Ð¸ Ð² Ð»ÐµÐ³ÐºÐ¸Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: The effect of silver nanoparticles on copper metabolism in the lungs
Creators: name: ÐÐ°Ð³Ð°Ð·ÐµÐ½ÐºÐ¾Ð²Ð°, ÐÐ°ÑÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Ð¡ÐµÑÐµÐ±ÑÐ¾
ÐÐµÐ´Ñ
ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð³ÐµÐ½Ð¾Ð²
Ð»ÐµÐ³ÐºÐ¸Ðµ
ÑÐµÑÑÐ»Ð¾Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð½
gene expression
lungs
ceruloplasmin

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«ÐÐ»Ð¸ÑÐ½Ð¸Ðµ Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸Ñ ÑÐµÑÐµÐ±ÑÐ° Ð½Ð° Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¼ Ð¼ÐµÐ´Ð¸ Ð² Ð»ÐµÐ³ÐºÐ¸ÑÂ». ÐÐ¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ AgNPs Ð¾Ð±Ð»Ð°Ð´Ð°ÑÑ Ð¿ÑÐ¾ÑÐ¸Ð²Ð¾Ð²Ð¸ÑÑÑÐ½ÑÐ¼ Ð´ÐµÐ¹ÑÑÐ²Ð¸ÐµÐ¼, Ð¾Ð´Ð½Ð°ÐºÐ¾ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼ Ð¸Ñ Ð·Ð°ÑÐ¸ÑÑ Ð¾Ñ Ð³ÑÐ¸Ð¿Ð¿Ð° Ð¸ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð½Ð° Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¼ Ð¼ÐµÐ´Ð¸ Ð² Ð»ÐµÐ³ÐºÐ¸Ñ Ð¸Ð·ÑÑÐµÐ½ Ð½ÐµÐ´Ð¾ÑÑÐ°ÑÐ¾ÑÐ½Ð¾. ÐÐ¾ÑÑÐ¾Ð¼Ñ ÑÐµÐ»ÑÑ Ð½Ð°ÑÑÐ¾ÑÑÐµÐ³Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¾ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ AgNPs Ð½Ð° Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¼ Ð¼ÐµÐ´Ð¸ Ð² Ð»ÐµÐ³ÐºÐ¸Ñ. ÐÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð¿Ð¾ÑÐ»Ðµ Ð¾Ð´Ð½Ð¾ÐºÑÐ°ÑÐ½Ð¾Ð³Ð¾ Ð²Ð½ÑÑÑÐ¸Ð±ÑÑÑÐ¸Ð½Ð½Ð¾Ð³Ð¾ Ð²Ð²ÐµÐ´ÐµÐ½Ð¸Ñ AgNPs ÑÐµÑÐµÐ±ÑÐ¾ Ð¿ÑÐ°ÐºÑÐ¸ÑÐµÑÐºÐ¸ ÑÑÐ°Ð·Ñ Ð¿Ð¾ÑÐ²Ð»ÑÐ»Ð¾ÑÑ Ð² ÐºÑÐ¾Ð²Ð¾ÑÐ¾ÐºÐµ, ÑÐµÑÐµÐ· ÑÐ°Ñ Ð´Ð¾ÑÑÐ¸Ð³Ð°Ð»Ð¾ Ð¼Ð°ÐºÑÐ¸Ð¼Ð°Ð»ÑÐ½Ð¾Ð¹ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸, Ð° Ð·Ð°ÑÐµÐ¼ Ð¿Ð¾ÑÑÐµÐ¿ÐµÐ½Ð½Ð¾ ÑÐ½Ð¸Ð¶Ð°Ð»Ð¾ÑÑ. Ð ÑÐºÐ°Ð½ÑÑ ÑÐµÑÐµÐ±ÑÐ¾ Ð½Ð°ÐºÐ°Ð¿Ð»Ð¸Ð²Ð°Ð»Ð¾ÑÑ Ð½ÐµÑÐ°Ð²Ð½Ð¾Ð¼ÐµÑÐ½Ð¾: Ð¿ÐµÑÐµÐ½Ñ Ð¸ ÑÐµÐ»ÐµÐ·ÐµÐ½ÐºÐ° Ð¾Ð±Ð»Ð°Ð´Ð°ÑÑ Ð½Ð°Ð¸Ð±Ð¾Ð»ÑÑÐ¸Ð¼Ð¸ Ð°ÐºÐºÑÐ¼ÑÐ»Ð¸ÑÑÑÑÐ¸Ð¼Ð¸ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑÐ¼Ð¸. ÐÑÐ¸ ÐµÐ¶ÐµÐ´Ð½ÐµÐ²Ð½Ð¾Ð¼ Ð²Ð²ÐµÐ´ÐµÐ½Ð¸Ð¸ AgNP ÑÐµÑÐµÐ±ÑÐ¾ Ð½Ð°ÐºÐ°Ð¿Ð»Ð¸Ð²Ð°ÐµÑÑÑ Ð² ÑÐºÐ°Ð½ÑÑ Ð¸ ÑÐµÑÐµÐ· 4 Ð´Ð½Ñ Ð´Ð¾ÑÑÐ¸Ð³Ð°ÐµÑ Ð¼Ð°ÐºÑÐ¸Ð¼Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð·Ð½Ð°ÑÐµÐ½Ð¸Ñ, ÐºÐ¾ÑÐ¾ÑÐ¾Ðµ Ð½Ðµ Ð¸Ð·Ð¼ÐµÐ½ÑÐµÑÑÑ Ð¿ÑÐ¸ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐ¸Ñ Ð¸Ð½ÑÐµÐºÑÐ¸ÑÑ. Ð¢Ð°ÐºÐ¶Ðµ Ð±ÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð´Ð»Ð¸ÑÐµÐ»ÑÐ½Ð¾Ðµ Ð²Ð²ÐµÐ´ÐµÐ½Ð¸Ðµ AgNP ÑÐ½Ð¸Ð¶Ð°ÐµÑ Ð½Ðµ ÑÐ¾Ð»ÑÐºÐ¾ Ð¾ÐºÑÐ¸Ð´Ð°Ð·Ð½ÑÑ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÑÑÐ²Ð¾ÑÐ¾ÑÐ¾ÑÐ½Ð¾Ð³Ð¾ Ð¦Ð, Ð½Ð¾ Ð¸ Ð¾ÐºÑÐ¸Ð´Ð°Ð·Ð½ÑÑ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ GPI-Cp. ÐÐ½Ð°Ð»Ð¸Ð· ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Ð¿Ð¾ÐºÐ°Ð·Ð°Ð», ÑÑÐ¾ Ð²Ð²ÐµÐ´ÐµÐ½Ð¸Ðµ AgNPs Ð¿ÑÐ¸Ð²Ð¾Ð´Ð¸Ñ Ðº ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ñ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð° Ð¼Ð ÐÐ Ð³ÐµÐ½Ð¾Ð² CTR1, Cp, SOD1, CCS, Mt Ð¸ PrP, Ð² ÑÐ¾ Ð²ÑÐµÐ¼Ñ ÐºÐ°Ðº ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð³ÐµÐ½Ð¾Ð² ATP7A, GPI-Cp Ð¸ ATOX Ð½Ðµ Ð¸Ð·Ð¼ÐµÐ½ÑÐµÑÑÑ. ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð±ÐµÐ»ÐºÐ¾Ð² Ð¿Ð¾ÐºÐ°Ð·Ð°Ð»Ð¾, ÑÑÐ¾ Ð²Ð²ÐµÐ´ÐµÐ½Ð¸Ðµ AgNPs ÑÐ²ÐµÐ»Ð¸ÑÐ¸Ð²Ð°ÐµÑ ÑÐ¸Ð½ÑÐµÐ· Ð±ÐµÐ»ÐºÐ° CTR1 Ð¸ ÑÐ½Ð¸Ð¶Ð°ÐµÑ Ð²ÑÑÐ°Ð±Ð¾ÑÐºÑ SOD1. Ð¢ÐµÐ¼ Ð½Ðµ Ð¼ÐµÐ½ÐµÐµ Ð¸Ð·Ð¼ÐµÑÐµÐ½Ð¸Ðµ SOD-Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð½Ðµ Ð²ÑÑÐ²Ð¸Ð»Ð¾ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð° Ð°ÐºÑÐ¸Ð²Ð½ÑÑ ÑÐ¾ÑÐ¼ Ð±ÐµÐ»ÐºÐ°. ÐÐ»Ñ GPI-Cp Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹ Ð² ÑÐ¸Ð½ÑÐµÐ·Ðµ Ð±ÐµÐ»ÐºÐ° Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¾ Ð½Ðµ Ð±ÑÐ»Ð¾.
description: The subject of the graduate qualification work is Â«The effect of silver nanoparticles on copper metabolism in the lungsÂ». It was shown that AgNPs have antiviral effect, however the mechanism of how they protect against influenza and how the interfere with copper metabolism in the lungs is poorly studied. Hence the aim of this study was determination of the effect of AgNPs on copper metabolism in the lungs. The gained data from current study illustrated that after one injection in peritoneal cavity, AgNPs almost immediately appeared in the blood circulation where it reaches its maximum concentration after one hour, and subsequently it decreases gradually. In tissues, silver accumulates unevenly, where the liver and spleen accumulate the biggest portions of silver ions. Upon daily administration of AgNPs, silver accumulates in tissues and after 4 days it reaches a maximum value, which does not increase with additional injections. Also, long-term administration of AgNPs decreases the oxidase activity of both Cp and GPI-Cp. Gene expression analysis showed that the introduction of AgNPs leads to an increase in the expression of the CTR1, Cp, SOD1, CCS, Mt and PrP genes while the expression of the ATP7A, GPI-Cp and ATOX genes does not change. Further investigation on the proteins expressed by the previous genes illustrated that the introduction of AgNPs increases the synthesis of the CTR1 protein. SOD1, on the contrary, despite the increase in the amount of mRNA showed a decrease in the amount of protein after the introduction of AgNPs. However, the measurement of SOD activity did not reveal changes in the number of active forms of protein. For GPI-Cp, no changes in protein synthesis were found.