cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Evaluation of an automated light transmission aggregometry
Creators: name: Ling, Li-qin
name: Liao, Juan
name: Niu, Qian
name: Wang, Xia
name: Jia, Jin
name: Zuo, Cheng-hua
name: Jiang, Hong
name: Zhou, Jing
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Biochemistry
subject: Medicine
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Hematology
subject: Infectious Diseases
subject: FOS: Health sciences

description: Light transmission aggregometry (LTA) is the “gold standard” for platelet function assessment, but it is time-consuming and labor intensive. Recently, an automated platelet aggregation method has been developed on a routine coagulation analyzer (Sysmex CS-2100i). In this study, the performances of CS-2100i including repeatability, correlation with a reference aggregometer (Chrono-log Model 700), and the threshold limitation of platelet counts in platelet-rich plasma (PRP) were evaluated for clinical use. The agonists were adenosine diphosphate (ADP), arachidonic acid, collagen, epinephrine, and ristocetin. The platelet concentration of PRP was adjusted with platelet-poor plasma (PPP) and physiological saline (PS). The CS-2100i showed an excellent repeatability and a strong correlation with the Chrono-log 700 in performing platelet aggregation, and its threshold limitation of platelet counts in PRP was 80 × 109/L. PPP had an inhibitory impact on platelet aggregation induced by ADP, arachidonic acid, collagen or epinephrine; while PS had an inhibitory impact on ristocetin-induced aggregation. PS should be used to adjust PRP for ADP-, arachidonic acid-, collagen-, or epinephrine-induced aggregation; while PPP was recommended for ristocetin-induced aggregation. The CS-2100i showed an excellent repeatability, a strong correlation with Chrono-log 700, a lower platelet count requirement, a shorter turnaround time for samples, the advantage of being a walk-away technology, and the ability to perform a highly standardized platelet function assessment.
Differential deposition of H2A.Z in rice seedling tissue during the day-night cycle
Creators: name: Zhang, Kang
name: Xu, Wenying
name: Wang, Chunchao
name: Yi, Xin
name: Su, Zhen
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Environmental Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Plant Biology

description: Chromatin structure has an important role in modulating gene expression. The incorporation of histone variants into the nucleosome leads to important changes in the chromatin structure. The histone variant H2A.Z is highly conserved between different species of fungi, animals, and plants. However, dynamic changes to H2A.Z in rice have not been reported during the day-night cycle. In this study, we generated genome wide maps of H2A.Z for day and night time in harvested seedling tissues by combining chromatin immunoprecipitation and high-throughput sequencing. The analysis results for the H2A.Z data sets detected 7099 genes with higher depositions of H2A.Z in seedling tissues harvested at night compared with seedling tissues harvested during the day, whereas 4597 genes had higher H2A.Z depositions in seedlings harvested during the day. The gene expression profiles data suggested that H2A.Z probably negatively regulated gene expression during the day-night cycle and was involved in many important biologic processes. In general, our results indicated that H2A.Z may play an important role in plant responses to the diurnal oscillation process.
Two new megastigmanes from Chinese traditional medicinal plant Sedum sarmentosum
Creators: name: Ma, Xinhua
name: Yang, Jie
name: Deng, Shihao
name: Huang, Mi
name: Zheng, Sijian
name: Xu, Shicheng
name: Cai, Jinyan
name: Yang, Xinzhou
name: Ai, Honglian
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Biochemistry
subject: Biotechnology
subject: Environmental Sciences not elsewhere classified
subject: Chemical Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Plant Biology
subject: FOS: Biological sciences

description: To discover new bioactive compounds from nature plants, a primary screening of traditional Chinese medicines had been taken. The screening results showed that a EtOAc extract of Sedum sarmentosum displayed a certain degree of cytotoxic activity and bioassay-directed isolation of EtOAc extract gave two new megastigmanes, (6S,9R)-2-hydroxy-4-(2,6,6-trimethyl-4-oxo-cyclohex-2-enyl)-butyric acid (1) and (6S,9R)-2-hydroxy-4-(2,6,6-trimethyl-4-oxo-cyclohex-2-enyl)-butyric acid methyl ester (2) together with seven known flavonoids. The chemical structures of 1 and 2 were elucidated on the basis of detailed 1D, 2D NMR and MS data. When tested against HepG2 and Hep3B hepatocellular carcinoma cell lines, compounds 1–9 showed weak anti-HCC activity. In addition, in vitro antioxidant activities of 1–9 were evaluated by ABTS radical cation-scavenging assay. 1 and 2 exhibited weak activity with per micromoles equivalent to 0.039 and 0.042 μM of Trolox, respectively. The flavonoid component, quercetin (9) showed the highest antioxidant activities with per micromoles equivalent 0.67 μM of Trolox.
Alashinols F and G, two lignans from stem bark of Syringa pinnatifolia
Creators: name: Feng, Xiao
name: Su, Guozhu
name: Ye, Yunyun
name: Zhang, Ruifei
name: Yang, Xinyao
name: Du, Bingzhao
name: Peng, Bing
name: Tu, Pengfei
name: Chai, Xingyun
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Biophysics
subject: Biochemistry
subject: Environmental Sciences not elsewhere classified
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: FOS: Biological sciences
subject: Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Science Policy
subject: Plant Biology

description: Two new lignans, alashinols F and G (1 and 2), together with two known analogues (−)-secoisolariciresinol (3) and meso-secoisolariciresinol (4) were isolated from the stem bark of Syringa pinnatifolia, a Mongolian folk medicine with anti-myocardial ischaemic effects. Their structures were elucidated on basis of spectroscopic data analyses, including MS and 1D and 2D NMR, and their absolute configurations were elucidated on the basis of experimental and calculated electronic circular dichroisms. The in vitro anti-inflammation and anti-hypoxia evaluations were also discussed.
Prognostic and discriminative power of the 7th TNM classification for patients with surgically treated papillary renal cell carcinoma: results of a multi-institutional validation study (CORONA subtype project)
Creators: name: May, Matthias
name: Surcel, Cristian
name: Capitanio, Umberto
name: Dell’Oglio, Paolo
name: Klatte, Tobias
name: Shariat, Shahrokh
name: Ecke, Thorsten
name: Wolff, Ingmar
name: Vergho, Daniel
name: Wagener, Nina
name: Huck, Nina
name: Pahernik, Sascha
name: Zastrow, Stefan
name: Wirth, Manfred
name: Borgmann, Hendrik
name: Haferkamp, Axel
name: Musquera, Mireia
name: Krabbe, Laura M.
name: Herrmann, Edwin
name: Scavuzzo, Anna
name: Mirvald, Cristian
name: Hutterer, Georg
name: Zigeuner, Richard
name: Stief, Christian G.
name: Waidelich, Raphaela
name: Cindolo, Luca
name: Kalusova, Krystina
name: Brookman-May, Sabine D.
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Medicine
subject: Cell Biology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Cancer
subject: Science Policy

description: Objective: Studies on the prognostic reliability of the Union for International Cancer Control tumor, node, metastasis (TNM) staging system for renal cell carcinoma (RCC) predominantly focus on clear-cell RCC. Therefore, the aim of this study was to investigate whether the oncological prognosis of surgically treated papillary RCC (papRCC) patients is reliably given by the current TNM system, by analyzing the largest database reported to date. Materials and methods: Data on 2325 papRCC patients who underwent surgical treatment in 1984– 2015 were collated from 17 international centers (median follow-up 47 months). Tumor stage was adapted to the 7th edition of the TNM system. Multivariable, bootstrap-corrected Cox regression models were applied to assess the independent impact of the TNM system on cancer-specific mortality (CSM) and all-cause mortality (ACM). Results: The median age at diagnosis was 63 years (interquartile range 54–70 years) and 77% of patients were male. Nephron-sparing surgery was performed in 42%, and 82% were with symptom free at diagnosis. In 6.7% (n = 156), organ metastasis (stage M1) was present at the time of surgery. On multivariable analysis, the TNM system and Fuhrman grade had an independent impact on both CSM and ACM, while patient age affected ACM only. The discriminative ability of the pT classification was significant for both endpoints: 5 year CSM rates were 5%, 17%, 36% and 56% for stages pT1, pT2, pT3 and pT4, respectively (each p p Conclusions: The 2010 TNM staging system can be reliably applied to papRCC patients and allows certain prognostic discrimination.
HydroShare: Advancing Hydrology through Collaborative Data and Model Sharing
Creators: name: Tarboton, David
name: Idaszak, Ray
name: Horsburgh, Jeffery
name: Ames, Dan
name: Goodall, Jon
name: Band, Larry
name: Merwade, Venkatesh
name: Couch, Alva
name: Hooper, Richard
name: Maidment, David
name: Dash, Pabitra
name: Stealey, Michael
name: Yi, Hong
name: Gan, Tian
name: Castronova, Tony
name: Miles, Brian
name: Li, Zhiyi
name: Morsy, Mohamed
name: Crawley, Shawn
name: Ramirez, Mauriel
name: Sadler, Jeff
name: Xue, Zhaokun
name: Lemann, Alex
name: Liu, Yan
name: Wang, Shaowen
name: Bandaragoda, Christina
Publisher: name: figshare
Year: 2017
Subjects: classificationCode: 370799
subject: Hydrology not elsewhere classified

description: HydroShare is an online, collaborative system for open sharing of hydrologic data, analytical tools, and models.  It supports the sharing of and collaboration around “resources” which are defined by standardized content types for data formats and models commonly used in hydrology.  These include time series, geographic grids and shapes, multidimensional space-time data as well as models and model instances. This poster illustrates the HydroShare collaborative environment and web based services developed to support the sharing and processing of hydrologic data and models.  With HydroShare you can: Share your data and models with colleagues; Manage who has access to the content that you share; Share, access, visualize and manipulate a broad set of hydrologic data types and models; Use the web services application programming interface (API) to program automated and client access; Publish data and models and obtain a citable digital object identifier (DOI); Aggregate your resources into collections; Discover and access data and models published by others; Use web apps to visualize, analyze and run models on data in HydroShare.  The capability to assign DOIs to HydroShare resources means that they are permanently citable helping researchers who share their data get credit for the data published.  Models, and Model Instances, which in HydroShare are a model application to a specific site with its input and output data can also receive DOI's.  Collections allow multiple resources from a study to be aggregated together providing a comprehensive archival record of the research outcomes, supporting transparency and reproducibility, thereby enhancing trust in the findings.  Reuse to support additional research is also enabled.  HydroShare supports web apps to act on resources for cloud (server) based visualization and analysis, including large scale geographic and digital elevation model analysis at the CyberGIS center at the National Center for Supercomputing Applications (NCSA) and execution of SWAT and RHESSys models.
Pharmacophore generation, atom-based 3D-QSAR and molecular dynamics simulation analyses of pyridine-3-carboxamide-6-yl-urea analogues as potential gyrase B inhibitors
Creators: name: M. A. Azam
name: J. Thathan
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Biophysics
subject: Biochemistry
subject: Pharmacology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: Cancer

description: DNA gyrase subunit B (GyrB) is an attractive drug target for the development of antibacterial agents with therapeutic potential. In the present study, computational studies based on pharmacophore modelling, atom-based QSAR, molecular docking, free binding energy calculation and dynamics simulation were performed on a series of pyridine-3-carboxamide-6-yl-urea derivatives. A pharmacophore model using 49 molecules revealed structural and chemical features necessary for these molecules to inhibit GyrB. The best fitted model AADDR.13 was generated with a coefficient of determination (r²) of 0.918. This model was validated using test set molecules and had a good r² of 0.78. 3D contour maps generated by the 3D atom-based QSAR revealed the key structural features responsible for the GyrB inhibitory activity. Extra precision molecular docking showed hydrogen bond interactions with key amino acid residues of ATP-binding pocket, important for inhibitor binding. Further, binding free energy was calculated by the MM-GBSA rescoring approach to validate the binding affinity. A 10 ns MD simulation of inhibitor #47 showed the stability of the predicted binding conformations. We identified 10 virtual hits by in silico high-throughput screening. A few new molecules were also designed as potent GyrB inhibitors. The information obtained from these methodologies may be helpful to design novel inhibitors of GyrB.
Two new biphenyls from the stems of Garcinia tetralata
Creators: name: Ji, Bing-Kun
name: Gao, Xue-Mei
name: Cui, Di
name: Wang, Shan-Shan
name: Huang, Wen-Zhong
name: Li, Yin-Ke
name: Mei, Shuang-Xi
name: Yang, Zhi
name: Li, Gan-Peng
name: Jiang, Meng-Yuan
name: He, Yong-Hui
name: Jiang, Zhi-Yong
name: Du, Gang
name: Pan, Xiao-Xia
name: Liu, Wen-Xing
name: Hu, Qiu-Fen
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Biochemistry
subject: Physical Sciences not elsewhere classified
subject: Molecular Biology
subject: Environmental Sciences not elsewhere classified
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: FOS: Biological sciences
subject: Biological Sciences not elsewhere classified
subject: Science Policy

description: Two new biphenyls (1 and 2) and three known xanthones (3–5) were isolated from the ethanol extract of the stems of Garcinia tetralata. Structural elucidations of 1–2 were elucidated by spectroscopic methods including extensive 1D- and 2D-nuclear magnetic resonance spectroscopy techniques. Compounds 1–2 showed anti-rotavirus activities with SI above 10.
МЕТОД ОБУЧЕНИЯ 4К: РАЗВИТИЕ КЛЮЧЕВЫХ НАВЫКОВ ДЛЯ УСПЕХА В СОВРЕМЕННОМ МИРЕ
Creators: name: Атаева, Гулсина
name: Курбанова, Дилнора
Publisher: name: Zenodo
Year: 2024

description: Современная образовательная парадигма претерпевает значительные изменения в ответ на вызовы быстро меняющегося мира. Одной из ключевых концепций, нацеленных на подготовку учащихся к успеху в XXI веке, является метод обучения 4К. Этот метод направлен на развитие четырех основных навыков: критическое мышление, креативность, коммуникация и кооперация (сотрудничество).
Social Ecological Variables of Coppice System - Montagna di Torricchio
Creators: name: [Unknown]
Publisher: name: FZ-Juelich B2SHARE
Year: 2020

description: Here below we provide and describe a data table with the quantitative means of socio-ecological variables relating to the area of ​​study and materials and method methods published in the following article: https://www.sciencedirect.com/science/article/pii/S037811271631057X Specifically, eight variables were included: road (the presence and condition of forestry roads), age, aspect (the compass direction a forest slope faces), diameter (tree diameter at chest height, or 1.30 m), height, slope, soil and tree cover. The Lter 'Montagna di Torricchio' site is part of the study area and includes the type of loggers defined as 'Comunanza Agraria'
Sago Palm Genome Size Estimation via Real-Time Quantitative PCR
Creators: name: Hairul Azman Roslan
Publisher: name: King Mongkut's Institute of Technology Ladkrabang
Year: 2020
Subjects: Genome size, Metroxylon sagu, real-time PCR, copy number, Pichia pastoris

description: Current Applied Science and Technology, 20, 2, 208-216
Study 2 Auxiliary Hypotheses
Creators: name: Garay, Maria
name: Urry, Heather L.
name: Hickey, Paige Connors
name: Raea E. Rasmussen
name: Eddine, Samer Nour
name: Pagan, Jeremy
Publisher: name: OSF Registries
Year: 2020

description: In this component, lab groups will document their survey item and hypothesis for the extension part of our direct replication-plus-extension of the De Neys et al. (2013) study.
Two copper(II)-bbtz modified Keggin and β-octamolybdate compounds captured in one-pot under hydrothermal conditions
Creators: name: Ying, Jun
name: Liu, Jia-Ni
name: Li, Ting-Ting
name: Tian, Yan
name: Ni, Huai-Ping
name: Ji, Xue-Bin
name: Liu, Guo-Cheng
name: Tian, Ai-Xiang
Publisher: name: Taylor & Francis
Year: 2017
Subjects: Biochemistry
Physical Sciences not elsewhere classified
Cell Biology
Chemical Sciences not elsewhere classified
Biological Sciences not elsewhere classified

description: Under hydrothermal conditions two compounds based on Keggin and β-octamolybdate, respectively, [Cu2(bbtz)4(SiMo12O40)] (1) and [Cu2(bbtz)4(Mo8O26)]1/2·H2O (2) (bbtz = 1,4-bis(1,2,4-triazol-1-ylmethyl)benzene), were synthesized in one-pot and characterized by single-crystal X-ray diffraction, elemental analyses, and IR spectroscopy. In compound 1, two adjacent wave-like CuII-bbtz lines are connected by SiMo12 anions and bbtz to generate a ladder-like chain. Adjacent chains share the same CuII ions to build a three-dimensional (3-D) framework. In compound 2, the β-Mo8 anions link Cu3(bbtz)2 subunits alternately to form a one-dimensional (1-D) chain. These chains connect each other through sharing CuII ions and a 3-D network is constructed. Moreover, we studied the electrocatalytic and photocatalytic activities of these two compounds. The results indicate that 1 and 2 show good electrocatalytic performance for the reduction of nitrite and bromate; they also exhibit photocatalytic properties for degradation of MB and RhB.
Risk for spontaneous preterm birth among inter-racial/ethnic couples*
Creators: name: Bat Zion Shachar
name: Mayo, Jonathan A.
name: Lyell, Deirdre J.
name: Stevenson, David K.
name: Shaw, Gary M.
name: Blumenfeld, Yair J.
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Medicine
subject: Neuroscience
subject: Physiology
subject: FOS: Biological sciences
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Sociology
subject: FOS: Sociology
subject: 111714 Mental Health
subject: FOS: Health sciences

description: Objective: Approximately 10% of US couples are inter-racial/ethnic. Substantial variation in preterm birth (PTB) rates is seen when stratified by race/ethnicity, although most studies focused solely on maternal racial/ethnic demographics. Our aims were to analyze the contribution of paternal in addition to maternal race/ethnicity, and to evaluate risk of spontaneous PTB for previously understudied inter-racial/ethnic couples. Methods: California singleton live births from 2007 to 2010 were included. Race/ethnicity was determined based on self-report, obtained from birth certificates and defined as African American (AA), Hispanic, Asian, and White. Logistic regression was used to estimate odds ratios of spontaneous PTB at 20–23, 24–31, 32–36 and <37 weeks of gestation, with White–White couples as reference. Results were stratified by previous PTB, pre-gestational and gestational diabetes and hypertension. To investigate the paternal contribution to the risk for any given maternal race/ethnicity we assessed the rates of PTB among inter-racial/ethnic couples compared to the respective same-race couple. Odds ratios were adjusted for maternal age, parity, BMI, prenatal care, payor status, education and smoking. Results: Among 1,664,939 live births, 13% (n = 216,417) were born to inter-racial/ethnic couples. Compared to White–White couples, risk for spontaneous PTB was increased across all inter-racial/ethnic couples with a non-White mother, except when the father was Asian. Patterns of association were similar after stratification by previous PTB, hypertension and diabetes. Paternal race/ethnicity was also a significant risk factor for PTB. Conclusions: Increased risks for spontaneous PTB were seen in most inter-racial/ethnic couple groupings. In addition to maternal race/ethnicity, paternal race/ethnicity was a significant risk factor in many inter-racial/ethnic couplings. Identifying such different risk profiles based on both maternal and paternal race/ethnicity may offer new lines of research inquiry for the underlying etiologies of PTB.
Hypermethylated LTR retrotransposon exhibits enhancer activity
Creators: name: Hu, Tianxiang
name: Zhu, Xingguo
name: Pi, Wenhu
name: Yu, Miao
name: Shi, Huidong
name: Tuan, Dorothy
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Environmental Sciences not elsewhere classified
subject: Chemical Sciences not elsewhere classified
subject: Science Policy
subject: Immunology
subject: FOS: Clinical medicine
subject: Developmental Biology
subject: Cancer
subject: Mental Health
subject: Hematology
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Computational Biology

description: LTR retrotransposons are repetitive DNA elements comprising ∼10% of the human genome. They are silenced by hypermethylation of cytosines in CpG dinucleotides and are considered parasitic DNA serving no useful function for the host genome. However, hypermethylated LTRs contain enhancer and promoter sequences and can promote tissue-specific transcription of cis-linked genes. To resolve the apparent paradox of hypermethylated LTRs possessing transcriptional activities, we studied the ERV-9 LTR retrotransposon located at the 5′ border of the transcriptionally active β-globin gene locus in human erythroid progenitor and erythroleukemia K562 cells. We found that the ERV-9 LTR, containing 65 CpGs in 1.7 kb DNA, was hypermethylated (with > 90% methylated CpGs). Hypermethylated LTR possessed transcriptional enhancer activity, since in vivo deletion of the LTR by CRISPR-cas9 suppressed transcription of the globin genes by > 50%. ChIP-qPCR and ChIP-seq studies showed that the hypermethylated LTR enhancer spanning recurrent CCAATCG and GATA motifs associated respectively with key transcription factors (TFs) NF-Y and GATA-1 and -2 at reduced levels, compared with the unmethylated LTR in transfected LTR-reporter gene plasmids. Electrophoretic mobility shift assays with methylated LTR enhancer probe showed that the methylated probe bound both NF-Y and GATA-1 and -2 with lower affinities than the unmethylated enhancer probe. Thus, hypermethylation drastically reduced, but did not totally abolish, the binding affinities of the enhancer motifs to the key TFs to assemble the LTR-pol II transcription complex that activated transcription of cis-linked genes at reduced efficiency.
LTSER Neajlov catchment biogeochemical parameters
Creators: name: [Unknown]
Publisher: name: FZ-Juelich B2SHARE
Year: 2020

description: Meteorological data for Neajlov LTSER site was recorded at Videle station for the period 2000-2016. Chemical parameters (June 2012 - June 2013) correspond to 3 sampling points located in Neajlov catchment. Plant species composition was compiled in 2012
Hypermethylated LTR retrotransposon exhibits enhancer activity
Creators: name: Hu, Tianxiang
name: Zhu, Xingguo
name: Pi, Wenhu
name: Yu, Miao
name: Shi, Huidong
name: Tuan, Dorothy
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Environmental Sciences not elsewhere classified
subject: Chemical Sciences not elsewhere classified
subject: Science Policy
subject: Immunology
subject: FOS: Clinical medicine
subject: Developmental Biology
subject: Cancer
subject: Mental Health
subject: Hematology
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Computational Biology

description: LTR retrotransposons are repetitive DNA elements comprising ∼10% of the human genome. They are silenced by hypermethylation of cytosines in CpG dinucleotides and are considered parasitic DNA serving no useful function for the host genome. However, hypermethylated LTRs contain enhancer and promoter sequences and can promote tissue-specific transcription of cis-linked genes. To resolve the apparent paradox of hypermethylated LTRs possessing transcriptional activities, we studied the ERV-9 LTR retrotransposon located at the 5′ border of the transcriptionally active β-globin gene locus in human erythroid progenitor and erythroleukemia K562 cells. We found that the ERV-9 LTR, containing 65 CpGs in 1.7 kb DNA, was hypermethylated (with >90% methylated CpGs). Hypermethylated LTR possessed transcriptional enhancer activity, since in vivo deletion of the LTR by CRISPR-cas9 suppressed transcription of the globin genes by >50%. ChIP-qPCR and ChIP-seq studies showed that the hypermethylated LTR enhancer spanning recurrent CCAATCG and GATA motifs associated respectively with key transcription factors (TFs) NF-Y and GATA-1 and −2 at reduced levels, compared to the unmethylated LTR in transfected LTR-reporter gene plasmids. Electrophoretic mobility shift assays with methylated LTR enhancer probe showed that the methylated probe bound both NF-Y and GATA-1 and −2 with lower affinities than the unmethylated enhancer probe. Thus, hypermethylation drastically reduced, but did not totally abolish, the binding affinities of the enhancer motifs to the key TFs to assemble the LTR-pol II transcription complex that activated transcription of cis-linked genes at reduced efficiency.
Preparation, characterization, and antioxidative activity of Bletilla striata polysaccharide/chitosan microspheres for oligomeric proanthocyanidins
Creators: name: Liu, Kang
name: Feng, Zhanqin
name: Shan, Lu
name: Yang, Tingting
name: Qin, Meng
name: Tang, Jinbao
name: Zhang, Weifen
Publisher: name: Taylor & Francis
Year: 2017
Subjects: Biophysics
Biochemistry
Pharmacology
Biotechnology
Chemical Sciences not elsewhere classified

description: Novel oligomeric proanthocyanidins/Bletilla striata polysaccharide/chitosan (OPC/BSP/CTS) microspheres were obtained by spray-drying method. The morphology, size, physicochemical characterization, and in vitro release behavior of the formulation were evaluated. The results of scanning electron microscopy showed OPC-loaded microspheres with considerably wrinkled surfaces and drug-free microspheres with smooth surfaces. Particle-size analysis showed that the diameters of the OPC/BSP/CTS microspheres were 1–5 µm, with uniform distribution (polydispersity below 0.465). Fourier transform infrared indicated the occurrence of a chemical reaction between BSP and CTS. The OPC formed intermolecular hydrogen bonds with BSP/CTS carriers or transmuted amorphous structure. Differential scanning calorimetry displayed the absence of interaction; OPC was only physically encapsulated in the polymer matrix and OPC changed the crystallinity. All the microspheres showed desirable biodegradation rates; the high CTS content retarded their degradation and extended administration times. OPC/BSP/CTS microspheres showed pronounced antioxidant activity than pure OPC. These microspheres can be used as a potential platform to efficiently deliver unstable drugs as a sustained-release oral drug delivery system.
68Ga-PSMA als Radiopharmakon für die Prostatakarzinom-Bildgebung
Creators: name: Cuber, Philipp Martin
Publisher: name: Universität Rostock
Year: 2018
Subjects: subject: 610 Medical sciences Medicine

description: In dieser Arbeit wurde die Expression von PSMA, die Kinetik der Internalisierung und der Einfluss von Docetaxel auf die Internalisierung des Radiopharmazeutikums 68Ga-PSMA an LNCaP- und PC3-Zellen in-vitro untersucht. Es erfolgte der Nachweis der Zielstruktur durch Western-Blot und Immunhistochemie. Weiterhin wurde nach Versuchen zur Festlegung der Standardbedingungen die Enzymkinetik nach Michaelis Menten sowie eine spezifische Hemmung untersucht. Abschließend wurde die Modulation durch das Chemotherapeutikum Docetaxel bezüglich einer Aufnahmemodulation untersucht.
Preparation, Characterization, and Antioxidative Activity of Bletilla striata Polysaccharide/Chitosan Microspheres for Oligomeric Proanthocyanidins
Creators: name: Liu, Kang
name: Feng, Zhanqin
name: Shan, Lu
name: Yang, Tingting
name: Qin, Meng
name: Tang, Jinbao
name: Zhang, Weifen
Publisher: name: Taylor & Francis
Year: 2017
Subjects: Biophysics
Biochemistry
Cell Biology
Neuroscience
Pharmacology
Biotechnology
Chemical Sciences not elsewhere classified

description: Novel oligomeric proanthocyanidins/Bletilla striata polysaccharide/chitosan (OPC/BSP/CTS) microspheres were obtained by spray-drying method. The morphology, size, physicochemical characterization and in vitro release behavior of the formulation were evaluated. The results of SEM showed OPC-loaded microspheres with considerably wrinkled surfaces and drug-free microspheres with smooth surfaces. Particle-size analysis showed that the diameters of the OPC/BSP/CTS microspheres were 1–5 µm, with uniform distribution (polydispersity below 0.465). FT-IR indicated the occurrence of a chemical reaction between BSP and CTS. The OPC formed intermolecular hydrogen bonds with BSP/CTS carriers or transmuted amorphous structure. DSC displayed the absence of interaction; OPC was only physically encapsulated in the polymer matrix and OPC changed the crystallinity. All the microspheres showed desirable biodegradation rates; the high CTS content retarded their degradation and extended administration times. OPC/BSP/CTS microspheres showed pronounced antioxidant activity than pure OPC. These microspheres can be used as a potential platform to efficiently deliver unstable drugs as a sustained-release oral drug delivery system.
Vegetation data of Rosalia Lehrforst, Austria, between the years 1977 and 1981
Creators: name: [Unknown]
Publisher: name: FZ-Juelich B2SHARE
Year: 2020

description: Vegetation data of Rosalia Lehrforst, Austria, between the years 1977 and 1981. Location (Lat, Lon) known within 100-200m
null tierexperimentelle Studie zur in-vivo-Testung eines biodegradierbaren Polylactid Stents aus PLLA/P4HB
Creators: name: Goosmann, Martin
Publisher: name: Universität Rostock
Year: 2018
Subjects: subject: 610 Medical sciences Medicine

description: In 5 Landschweine wurde jeweils ein biodegradierbarer PLLA/P4HB-Stent und ein Metall-Stent in die linke beziehungsweise rechte A. iliaca com. implantiert, 6 Wochen belassen und nach Tötung der Tiere explantiert und sowohl histologisch als auch planimetrisch untersucht. Hierbei zeigte sich eine gesteigerte Entzündungsreaktion sowie vermehrtes Gewebewachstum um die Polylactid-Stents, was zu einem reduzierten Lumen führte. Ein kompletter Verschluss trat jedoch nicht auf. Der neue Stent war sicher zu implantieren, die Optimierung der Biokompatibilität bedarf jedoch weiterer Forschung.
Gelling properties of myosin as affected by L-lysine and L-arginine by changing the main molecular forces and microstructure
Creators: name: Fu, Yuan
name: Yadong Zheng
name: Lei, Zhen
name: Xu, Peng
name: Cunliu Zhou
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Biochemistry
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Evolutionary Biology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified

description: This paper investigated the effects of l-lysine (Lys) and l-arginine (Arg) on the water-holding capacity (WHC) and hardness of myosin gel as well as the mechanism of these effects. The results showed that Lys, Arg, and KOH increased the WHC but decreased the hardness in all cases. At pH 6.32, the WHC increased in the order control < KOH < Lys ≈ Arg, while the hardness decreased in the order control > KOH > Lys > Arg. Lys shortened low field nuclear magnetic resonance spin-spin relaxation times (T2), while Arg and KOH slightly affected T2. Lys, Arg, and KOH had different influences on the molecular forces in myosin gels and the distribution of myosin size. Lys formed a gel with porous clusters, while Arg generated a gel with fine caves. Therefore, both Lys and Arg changed the microstructure of myosin gel by changing the distribution of the myosin size as well as the molecular forces that form and/or maintain myosin gel, ultimately contributing to the differences in the WHC, hardness, and T2.
Braila Islands LTSER site (LTER_EU_RO_006) chemical parameters
Creators: name: [Unknown]
Publisher: name: FZ-Juelich B2SHARE
Year: 2020

description: The data is for the Braila Islands LTSER site (LTER_EU_RO_006), for the following chemical parameters : NH4N, NO3N, NO2N, Ntot, SS, PH, OM, HH, PO4P, Ptot, for the years 2006, 2008, 2009, 2011, 2013
Expression profile of SIRT2 in human melanoma and implications for sirtuin-based chemotherapy
Creators: name: Wilking-Busch, Melissa Jean
name: Ndiaye, Mary Ann
name: Huang, Wei
name: Ahmad, Nihal
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Physiology
subject: Pharmacology
subject: Environmental Sciences not elsewhere classified
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Cancer

description: Melanoma is cancer of melanin-containing melanocyte cells. This neoplasm is one of the most deadly forms of skin cancer, and currently available therapeutic options are insufficient in significantly improve outcomes for many patients. Therefore, novel targets are required to effectively manage this neoplasm. Several sirtuins have previously been found to be upregulated in melanoma, so in this study, the expression profile of SIRT2 was determined. Employing a tissue microarray containing benign nevi, primary melanomas, and lymph node metastases, we have found that the tissue from lymph node metastases appears to have a significant upregulation of SIRT2 relative to primary tumors across the nuclear, cytoplasmic, and whole cell data. Additionally, SIRT2 staining was found to be higher in the nucleus of metastatic melanomas compared to cytoplasmic staining. As SIRT2 is considered to be a predominantly cytoplasmic protein, this is a novel and very interesting finding. This, combined with previous studies that show other sirtuins are increased in melanoma and involved in cellular proliferation and survival, leads to the suggestion that exploring pan-sirtuin inhibitors may be the best target for the next iteration of melanoma chemotherapeutics.
Expression Profile of SIRT2 in Human Melanoma and Implications for Sirtuin-Based Chemotherapy
Creators: name: Wilking-Busch, Melissa Jean
name: Ndiaye, Mary Ann
name: Huang, Wei
name: Ahmad, Nihal
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Physiology
subject: Pharmacology
subject: Environmental Sciences not elsewhere classified
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Cancer

description: Melanoma is cancer of melanin-containing melanocyte cells. This neoplasm is one of the most deadly forms of skin cancer, and currently available therapeutic options are insufficient in significantly improve outcomes for many patients. Therefore, novel targets are required to effectively manage this neoplasm. Several sirtuins have previously been found to be upregulated in melanoma, so in this study, the expression profile of SIRT2 was determined. Employing a tissue microarray containing benign nevi, primary melanomas, and lymph node metastases, we have found that the tissue from lymph node metastases appears to have a significant upregulation of SIRT2 relative to primary tumors across the nuclear, cytoplasmic, and whole cell data. Additionally, SIRT2 staining was found to be higher in the nucleus of metastatic melanomas compared to cytoplasmic staining. As SIRT2 is considered to be a predominantly cytoplasmic protein, this is a novel and very interesting finding. This, combined with previous studies that show other sirtuins are increased in melanoma and involved in cellular proliferation and survival, leads to the suggestion that exploring pan-sirtuin inhibitors may be the best target for the next iteration of melanoma chemotherapeutics.
Gelling Properties of Myosin as Affected by l-Lysine and l-Arginine by Changing the Main Molecular Forces and Microstructure
Creators: name: Fu, Yuan
name: Yadong Zheng
name: Lei, Zhen
name: Xu, Peng
name: Cunliu Zhou
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Biochemistry
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Evolutionary Biology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified

description: This paper investigated the effects of l-lysine (Lys) and l-arginine (Arg) on the water-holding capacity (WHC) and hardness of myosin gel as well as the mechanism of these effects. The results showed that Lys, Arg and KOH increased the WHC but decreased hardness in all cases. At pH 6.32, the WHC increased in the order control < KOH < Lys ≈ Arg, while the hardness decreased in the order control > KOH > Lys > Arg. Lys shortened low field nuclear magnetic resonance spin-spin relaxation times (T2), while Arg and KOH slightly affected T2. Lys, Arg and KOH had different influences on the molecular forces in myosin gels and the distribution of the myosin size. Lys formed a gel with porous clusters, while Arg generated a gel with fine caves. Therefore, both Lys and Arg changed the microstructure of myosin gel by changing the distribution of the myosin size as well as the molecular forces that form and/or maintain myosin gel, ultimately contributing to the differences in the WHC, hardness and T2.
Toxicity assessment of molindone hydrochloride, a dopamine D2/D5 receptor antagonist in juvenile and adult rats
Creators: name: Gopala Krishna
name: Gopa Gopalakrishnan
name: Saryu Goel
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Biochemistry
subject: Medicine
subject: Neuroscience
subject: Physiology
subject: FOS: Biological sciences
subject: Pharmacology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: Developmental Biology
subject: Science Policy

description: Neuroleptic drug molindone hydrochloride is a dopamine D2/D5 receptor antagonist and it is in late stage development for the treatment of impulsive aggression in children and adolescents who have attention deficient/hyperactivity disorder (ADHD). This new indication for this drug would expand the target population to include younger patients, and therefore, toxicity assessments in juvenile animals were undertaken in order to determine susceptibility differences, if any, between this age group and the adult rats. Adult rats were administered molindone by oral gavage for 13 weeks at dose levels of 0, 5, 20, or 60 mg/kg-bw/day. Juvenile rats were dosed for 8 weeks by oral gavage at dose levels of 0, 5, 25, 50, or 75 mg/kg-bw/day. Standard toxicological assessments were made using relevant study designs in consultation with FDA. Treatment-related elevation in serum cholesterol and triglycerides and decreases in glucose levels were observed in both the age groups. Organ weight changes included increases in liver, adrenal gland and seminal vesicles/prostate weights. Decreases in uterine weights were also observed in adult females exposed to the top two doses with sufficient exposure. In juveniles, sexual maturity parameters secondary to decreased body weights were observed, but, were reversed. In conclusion, the adverse effects noted in reproductive tissues of adults were attributed to hyperprolactinemia and these changes were not considered to be relevant to humans due to species differences in hormonal regulation of reproduction. On the whole, there were no remarkable differences in the toxicity profile of the drug between the two age groups.
Designing the Part-Whole Self
Creators: name: Rodighiero, Dario
Publisher: name: Zenodo
Year: 2020

description: According to the mathematical theory of communication, the circulation of information is given by a double translation that corresponds to two phases of encoding and decoding (Shannon 1948). With respect to data visualization, encoding translates data into graphic elements and decoding takes place during the interpretation given by the reader. Considering the self as the basic element of Claude Shannon’s theory implies a serious reflection about the design process employed to create visualizations. How to present individuals? What is the best way to present a community? Are minorities properly represented in visual artifacts? What is the relationship between the individual and his/her community? All these questions belong to the ethical sphere of design. When we adopt, in particular, network visualizations to represent small and large communities using the relational paradigm, these questions can be addressed more precisely. Social relations (edges) connect individuals (nodes) through well-known libraries such as d3.js, giving a graphic form to a whole community on laptop screens, smartphones, and tablets. Network visualizations represent communities by individuals, as a visual re-appropriation of the Aristotelian part-whole theory. Yet design is a personal practice (Drucker 2014) that charges the designer with specific questions and brings to practical actions. How to draw nodes to present individuals? Which metrics can fairly space out individuals? How to manage the comparison between nodes and visual centrality? And how to give visibility to minorities? This proposal is based on my Ph.D. dissertation (Rodighiero 2018) and my postdoctoral studies (Moon and Rodighiero 2020) that focus on the design as an ethical process to display communities and their members by making use of network visualizations, with a keen interest in both self and collective representation.
Studies list.docx
Creators: name: Rossi, Rodolfo
Publisher: name: figshare
Year: 2017
Subjects: subject: 111716 Preventive Medicine
subject: FOS: Health sciences
subject: 111402 Obstetrics and Gynaecology
subject: FOS: Clinical medicine
subject: 110804 Medical Virology
subject: 110709 Tumour Immunology

description: List of studies retrieved to perform a systematic review exploring the impact of HAART on HPV infection, onset of cervical abnormal cytology and consequent cervical cancer.
Total synthesis of an isoflavone C-glycoside: 6-tert-butylpuerarin
Creators: name: Zou, Yunpeng
name: Zhang, Shouguo
name: Wang, Gang
name: Wen, Xiaoxue
name: Liu, Shuchen
name: Peng, Tao
name: Gao, Yue
name: Wang, Lin
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Microbiology
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Plant Biology
subject: Computational Biology

description: The first total synthesis of an isoflavone C-glycoside (6-tert-butylpuerarin) using commercially available 4,6-di-tert-butylbenzene-1,3-diol as starting material was achieved in five steps with an overall yield of 2.8%. The key intermediate 4 was obtained by de-tert-butylation of 2 with trifluoroacetic acid and Friedel-Crafts acetylation of 2-C-β-D-glucopyranoside 3. Condensation of 4 with 4-(benzyloxy)benzaldehyde resulted in the formation of C-glucosylchalcone 5, which was cyclized by oxidative rearrangement using (diacetoxyiodo)benzene (DIB) and p-toluenesulfonic acid to obtain the target molecule 6. This environmentally friendly and concise synthetic pathway should be applicable to the large-scale synthesis of various isoflavone C-glycosides.
Park Shaked Long-Term Socio-ecological Research Platform: LTER_EU_IL_001:Basic climate at Park Shaked LTSER Platform (1999-2018)
Creators: name: [Unknown]
Publisher: name: FZ-Juelich B2SHARE
Year: 2020

description: Meteorology data for Park Shaked - Israel Park Shaked LTER station (31.267 N, 34.653 E) Average Air Temp Maximum Air Temp Minimum Air Temp Maximun Relative Humidity Minimum Relative Humidity Maximum Temparture Deficit Minimum Temparture Deficit Average Vapor Pressure Deficit Average Vapor Pressure Maximum Barometric Pressure Minimum Barometric Pressure Maximum Solar Insolation Total Solar Radiation Average Wind Speed Maximum Wind Speed Ground Temp. 25cm depth Ground Temp. 50cm depth Ground Moisture 25cm depth Ground Moisture 50cm depth Total Rain Min DataLogger Battery Level Min Cellphone Battery Level
RO_SI000726_Meteorological_data_2018.08.24
Creators: name: [Unknown]
Publisher: name: FZ-Juelich B2SHARE
Year: 2020

description: The data is for the Braila Islands LTSER site (LTER_EU_RO_006) for the meteorological data recorded at the Braila weather station, for the period 2000-2016. Plant species composition was compiled in 2007.
Selection on flower size in Kielmeyera
Creators: name: Teixido, Alberto
Publisher: name: figshare
Year: 2017
Subjects: subject: 60299 Ecology not elsewhere classified
subject: FOS: Biological sciences
subject: 60399 Evolutionary Biology not elsewhere classified
subject: Botany

description: Differential gender selection on flower size in two Neotropical savanna congeneric species
خدمات المعلومات عن بعد في المكتبات الجامعية دراسة ميدانية
Creators: name: سويفي،رحاب عبدالهادي
Publisher: name: Zenodo
Year: 2021
Subjects: المكتبات الجامعية | خدمة المعلومات عن بعد | خدمات المعلومات | University Libraries | Remote Information Services | Information Services

description: المكتبة كمؤسسة اجتماعية تتأثر بالتغيرات والتحديات التي تواجه المجتمعات ولعل جائحة كورونا وما تبعه من إغلاق للمكتبات ومؤسسات المعلومات وإغلاق الخدمات مؤقتا أو تقليلها إلى الحد الأدنى للمساهمة في الجهود المبذولة للحد من انتشار المرض ويمكن القول بأن تعبير إغلاق المكتبة ليس هو التعبير الدقيق أو على الأقل الأنسب، فقد تكون مباني المكتبات قد أغلقت، ولكن المكتبات ككيان ماتزال مفتوحة، خاصة مع إمكانية عمل موظفي المكتبات عن بعد من منازلهم اعتمادا على البنية التقنية المتطورة وتدفق سير العمل المتماسك والانسيابي في نفس الوقت، بل وقدمت بعض المكتبات خدماتها بشكل غير تقليدي ولا يتطلب حضور المستفيد إلى المكتبة، ومن هنا تأتى هذه الدراسة للتعرف على تلك الخدمات التي تقدمها المكتبات الجامعية عن بعد، ومدى رضا المستفيدين عن تقديم تلك الخدمات، وذلك بالتعرف على بعض النماذج للمكتبات ومراكز المعلومات التي تقدم خدماتها عن بعد، ومقومات تقديم هذه النوعية من الخدمات، والمهارات اللازم توافرها في أخصائي المعلومات التي يقوم بأداء تلك المهمة.