title: Ð¤ÑÐ½ÐºÑÐ¸Ð¾Ð½Ð°Ð»ÑÐ½Ð°Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÑÐ»ÐµÐºÑÑÐ¾Ð²Ð¾Ð·Ð±ÑÐ´Ð¸Ð¼ÑÑ Ð½Ð°ÑÑÐ¸ÐµÐ²ÑÑ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² Ð² ÐºÐ»ÐµÑÐºÐ°Ñ Ð»Ð¸Ð½Ð¸Ð¸ Ðº562 Ð¸ Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Functional activity of excitable sodium channels in k562 cell line and human mesenchymal stem cells
Creators: name: Ð¨Ð°Ð¼Ð°ÑÐ¾Ð²Ð°, ÐÐ°ÑÐ³Ð°ÑÐ¸ÑÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: ÐºÐ»ÐµÑÐºÐ¸ Ð»ÐµÐ¹ÐºÐµÐ¼Ð¸Ð¸ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð562
ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ð°Ð»ÑÐ½ÑÐµ
Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÐµ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸
ÑÐ»ÐµÐºÑÑÐ¾Ð²Ð¾Ð·Ð±ÑÐ´Ð¸Ð¼ÑÐµ Ð½Ð°ÑÑÐ¸ÐµÐ²ÑÐµ ÐºÐ°Ð½Ð°Ð»Ñ
Ð¼Ð¸Ð³ÑÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ
human leukemia cells K562
endometrial mesenchymal stem cells
voltage-gated sodium channels
migration ability

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð°Ð»ÑÐ½Ð¾Ð¹ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ ÑÐ»ÐµÐºÑÑÐ¾Ð²Ð¾Ð·Ð±ÑÐ´Ð¸Ð¼ÑÑ Ð½Ð°ÑÑÐ¸ÐµÐ²ÑÑ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² (Nav) Ð² ÐºÐ»ÐµÑÐºÐ°Ñ Ð»ÐµÐ¹ÐºÐµÐ¼Ð¸Ð¸ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð562 Ð¸ Ð² ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ð°Ð»ÑÐ½ÑÑ Ð¼ÐµÐ·ÐµÐ½ÑÐ¸Ð¼Ð½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° (ÑÐÐ¡Ð), Ð° ÑÐ°ÐºÐ¶Ðµ Ð² Ð¿ÑÐ¾Ð²ÐµÑÐºÐµ Ð¿ÑÐµÐ´Ð¿Ð¾Ð»Ð¾Ð¶ÐµÐ½Ð¸Ð¹ Ð¾Ð± ÑÑÐ°ÑÑÐ¸Ð¸ NaV Ð² ÑÐµÐ³ÑÐ»ÑÑÐ¸Ð¸ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ Ð¿Ð¾Ð´Ð²Ð¸Ð¶Ð½Ð¾ÑÑÐ¸ ÑÐÐ¡Ð. Ð ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ñ, Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð½ÑÑ Ð² ÑÐµÐ¶Ð¸Ð¼Ðµ Ð¾ÑÐ²ÐµÐ´ÐµÐ½Ð¸Ñ ÑÐ¸Ð³Ð½Ð°Ð»Ð¾Ð² Ð¾Ñ ÑÐµÐ»Ð¾Ð¹ ÐºÐ»ÐµÑÐºÐ¸ (whole-cell) Ð¼ÐµÑÐ¾Ð´Ð° patch-clamp Ð½Ð° ÐºÐ»ÐµÑÐºÐ°Ñ Ð562 Ð¸ ÑÐÐ¡Ð ÑÐµÐ³Ð¸ÑÑÑÐ¸ÑÐ¾Ð²Ð°Ð»Ð¸ Ð¸Ð¾Ð½Ð½ÑÐµ ÑÐ¾ÐºÐ¸ Ð²ÑÐ¾Ð´ÑÑÐµÐ³Ð¾ Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð¸Ñ, Ð°ÐºÑÐ¸Ð²Ð¸ÑÑÐµÐ¼ÑÐµ Ð¿ÑÐ¸ Ð´ÐµÐ¿Ð¾Ð»ÑÑÐ¸Ð·Ð°ÑÐ¸Ð¸Ð¸ Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ñ Ð¸ Ð¾Ð±Ð»Ð°Ð´Ð°ÑÑÐ¸Ðµ ÐºÐ¸Ð½ÐµÑÐ¸ÐºÐ¾Ð¹, ÑÐ°ÑÐ°ÐºÑÐµÑÐ½Ð¾Ð¹ Ð´Ð»Ñ ÑÐ»ÐµÐºÑÑÐ¾Ð²Ð¾Ð·Ð±ÑÐ´Ð¸Ð¼ÑÑ Ð½Ð°ÑÑÐ¸ÐµÐ²ÑÑ ÐºÐ°Ð½Ð°Ð»Ð¾Ð². ÐÑÐµ Ð·Ð°ÑÐµÐ³Ð¸ÑÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐµ ÐºÐ°Ð½Ð°Ð»Ñ Ð±Ð»Ð¾ÐºÐ¸ÑÐ¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐµÑÐºÐ¸Ð¼ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾ÑÐ¾Ð¼ Ð¢Ð¢Ð¥, ÑÑÐ¾ ÑÐ»ÑÐ¶Ð¸Ñ Ð´Ð¾ÐºÐ°Ð·Ð°ÑÐµÐ»ÑÑÑÐ²Ð¾Ð¼ ÑÐ¾Ð³Ð¾, ÑÑÐ¾ Ð´Ð°Ð½Ð½ÑÐµ ÐºÐ°Ð½Ð°Ð»Ñ ÑÐ²Ð»ÑÑÑÑÑ NaV. Ð¤ÑÐ½ÐºÑÐ¸Ð¾Ð½Ð°Ð»ÑÐ½Ð°Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² Ð±ÑÐ»Ð° Ð¿Ð¾Ð´ÑÐ²ÐµÑÐ¶Ð´ÐµÐ½Ð° Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¸Ð¼Ð¼ÑÐ½Ð¾ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð³Ð¾ Ð¾ÐºÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ñ ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐµÑÐºÐ¸Ð¼Ð¸ Ð°Ð½ÑÐ¸ÑÐµÐ»Ð°Ð¼Ð¸ Ðº ÑÑÐ±ÑÐµÐ´Ð¸Ð½Ð¸ÑÐ°Ð¼ NaV ÐºÐ°Ð½Ð°Ð»Ð°. ÐÐ»Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¼Ð¸Ð³ÑÐ°ÑÐ¸Ð¾Ð½Ð½ÑÑ ÑÐ²Ð¾Ð¹ÑÑÐ² ÐºÐ»ÐµÑÐ¾Ðº Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Â«Ð·Ð°Ð¶Ð¸Ð²Ð»ÐµÐ½Ð¸Ñ ÑÐ°Ð½ÑÂ» (scratch-ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÑ). Ð­ÐºÑÐ¿ÑÐµÑÑÐ¸Ñ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² NaV ÑÐ²ÑÐ·Ð°Ð½Ð° Ñ Ð¸Ð½Ð²Ð°Ð·Ð¸Ð²Ð½Ð¾ÑÑÑÑ Ð½ÐµÐºÐ¾ÑÐ¾ÑÑÑ ÑÐ¸Ð¿Ð¾Ð² ÑÐ°ÐºÐ¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº, Ð² ÑÐ¾Ð¼ ÑÐ¸ÑÐ»Ðµ Ð¸ Ð² Ð562. ÐÐµÐ·ÐµÐ½ÑÐ¸Ð¼Ð½ÑÐµ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÐµ Ð¸Ð· Ð´ÐµÑÐºÐ²Ð°Ð¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ñ (ÑÐÐ¡Ð), Ð¼Ð¾Ð³ÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°ÑÑÑÑ Ð² ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐµÑÐ°Ð¿Ð¸Ð¸. ÐÐ¾ÑÑÐ¾Ð¼Ñ Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ ÑÐ¾Ð»Ð¸ Ð¸Ð¾Ð½Ð½ÑÑ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² Ð² Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ ÑÐ²Ð»ÑÐµÑÑÑ Ð°ÐºÑÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð·Ð°Ð´Ð°ÑÐµÐ¹.
description: This work is devoted to the study of the functional expression of excitable sodium channels (Nav) in human leukemia Ð562 cells and in human endometrial mesenchymal stem cells (eMSCs), and testing the assumption about the participation of Nav in the regulation of cellular mobility of eMSCs. In whole-cell experiments of the patch-clamp method on Ð562 and eMSC cells, inward ion currents, activated by depolarization of the membrane and have the kinetics which is typical for excitable sodium channels, were recorded. All registered channels were blocked by a specific inhibitor TTX, which serves as evidence that these channels are Nav. Functional channel expression was confirmed by immunofluorescent staining with specific antibodies to the Nav channel subunits. To study the migratory properties of cells, the âwound healingâ assay (scratch experiments) was used. The expression of Nav channels is associated with invasiveness of some types of cancer cells, including K562. Human mesenchymal stem cells derived from desquamated endometrium (eMSC) can be used in cell therapy. Therefore, the study of the role of ion channels in the studied cells is an actual task.