title: ÐÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²ÐµÐ½Ð½Ð¾Ð¹ MALDI Ð¼Ð°ÑÑ-ÑÐ¿ÐµÐºÑÑÐ¾Ð¼ÐµÑÑÐ¸Ð¸ Ð´Ð»Ñ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ Ð°Ð»ÑÑÐ°-2-Ð¼Ð°ÐºÑÐ¾Ð³Ð»Ð¾Ð±ÑÐ»Ð¸Ð½Ð° Ð¸ ÑÐ¸Ð±ÑÐ¸Ð½Ð¾Ð¿ÐµÐ¿ÑÐ¸Ð´Ð° Ð Ð² ÑÑÐ²Ð¾ÑÐ¾ÑÐºÐµ ÐºÑÐ¾Ð²Ð¸ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Application of quantitative MALDI mass spectrometry to determine the concentration of alpha-2-macroglobulin and fibrinopeptide A in human serum
Creators: name: Ð¢ÑÐ»Ð¸Ð½, ÐÐ»ÐµÐºÑÐ°Ð½Ð´Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: Ð²Ð¾ÑÐ¿Ð°Ð»ÐµÐ½Ð¸Ðµ
ÑÐµÐ¿ÑÐ¸Ñ
Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐ°
ÑÑÐ²Ð¾ÑÐ¾ÑÐ¾ÑÐ½ÑÐµ Ð±Ð¸Ð¾Ð¼Ð°ÑÐºÐµÑÑ
ÑÐ¸Ð±ÑÐ¸Ð½Ð¾Ð¿ÐµÐ¿ÑÐ¸Ð´ Ð
Ð°Ð»ÑÑÐ°-2-Ð¼Ð°ÐºÑÐ¾Ð³Ð»Ð¾Ð±ÑÐ»Ð¸Ð½
ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²ÐµÐ½Ð½Ð°Ñ Ð¼Ð°ÑÑ-ÑÐ¿ÐµÐºÑÑÐ¾Ð¼ÐµÑÑÐ¸Ñ
MALDI-TOF Ð¼Ð°ÑÑ-ÑÐ¿ÐµÐºÑÑÐ¾Ð¼ÐµÑÑÐ¸Ñ
inflammation
sepsis
diagnostics
serum biomarkers
fibrinopeptide a
alpha-2-macroglobulin
quantitative mass spectrometry
MALDI-TOF mass spectrometry

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description: The topic of the graduation work: âApplication of quantitative MALDI mass spectrometry to determine the concentration of alpha-2-macroglobulin and fibrinopeptide A in human serum". This work is devoted to the development of a method for the quantitative assessment of the concentrations of such inflammatory markers as alpha-2-macroglobulin and fibrinopeptide A in blood serum using MALDI-TOF mass spectrometry with an isotopically labeled internal standards. As a result of the work, the peptides included in the considered markers were identified, and isotopically labeled standards were obtained from the synthesized analogs. The optimal detection time for the considered compounds was determined, and the features of the enzymatic reaction of trypsin with alpha-2-macroglobulin and alpha-1-antitrypsin -the second most important protease inhibitor in human serum -were established. In conclusion, the method for quantitative assessment of serum levels of alpha-2-macroglobulin and fibrinopeptide A using modern mass spectrometric approaches has been developed and verified. It allows analysis in blood serum withoutitspreliminary fractionation. The proposed methodis simpler to implement in comparison with standard methods of quantitative analysis of serum markers, and also faster, since the total analysis time is about 1 hour from the moment of obtaining a sample of the patient's blood serum to quantifying the levels of the considered compounds.