title: Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð°Ð»Ð³Ð¾ÑÐ¸ÑÐ¼Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¼Ð° ÐºÑÐµÐ½Ð¾Ð±Ð¸Ð¾ÑÐ¸ÐºÐ¾Ð² in vitro
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Development of an algorithm for studying the metabolism of xenobiotics in vitro
Creators: name: ÐÐ»ÑÑÑÐ¾Ð½Ð¾Ðº, Ð¡ÐµÐ¼ÑÐ½
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: ÑÐ»ÐµÐºÑÑÐ¾Ñ Ð¸Ð¼Ð¸ÑÐµÑÐºÐ¾Ðµ Ð¾ÐºÐ¸ÑÐ»ÐµÐ½Ð¸Ðµ
ÑÐ¾ÑÐ¾ÐºÐ°ÑÐ°Ð»Ð¸ÑÐ¸ÑÐµÑÐºÐ¾Ðµ Ð¾ÐºÐ¸ÑÐ»ÐµÐ½Ð¸Ðµ Ð² ÑÑÑÐ¿ÐµÐ½Ð·Ð¸Ð¸ Ð´Ð¸Ð¾ÐºÑÐ¸Ð´Ð° ÑÐ¸ÑÐ°Ð½Ð°
Ð±ÐµÑÐºÐ°Ð¿ÐµÐ»ÑÐ½Ð¾Ðµ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ°ÑÐ¿ÑÐ»ÐµÐ½Ð¸Ðµ
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ÐÐÐÐÐ Ð¼Ð°ÑÑ-ÑÐ¿ÐµÐºÑÑÐ¾Ð¼ÐµÑÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð°Ð½Ð°Ð»Ð¸Ð·
electrochemical oxidation
photocatalytic oxidation in titanium dioxide suspension
dropless electrospray
LC-mass spectrometric analysis
MALDI mass spectrometric analysis

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description: The work is aimed at developing and optimizing methods for modeling the biotransformation of xenobiotics, which make it possible to identify substances capable of forming reactive metabolites. Objective: development of a method for rapid assessment of the possibility of formation of reactive metabolites Tasks solved during the research: 1. Obtaining reactive metabolites of xenobiotics by electrochemical oxidation and photocatalytic oxidation using a suspension of titanium dioxide. 2. Development of a method for photocatalytic oxidation of xenobiotics on MALDI targets. 3. Evaluation of the ability of the obtained metabolites to form adducts with glutathione and human globin. 4. Identification of oxidation products and adducts using mass spectrometric analysis. 5. Comparison of the efficiency of obtaining reactive metabolites for quick assessment of drug toxicity. The work was carried out at the Federal State-Financed Institution Golikov Research Clinical Center of Toxicology under the Federal Medical Biological Agency. As a result, an electrospray setup was assembled, and a fast and efficient method for modeling xenobiotic metabolism on the surface of a MALDI target functionalized with titanium dioxide was developed.