Additional file 1 of Direct 3D printed biocompatible microfluidics: assessment of human mesenchymal stem cell differentiation and cytotoxic drug screening in a dynamic culture system
Creators: name: Riester, Oliver
name: Laufer, Stefan
name: Deigner, Hans-Peter
Publisher: name: figshare
Year: 2024
Subjects: subject: Space Science
subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Biotechnology
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Hematology

description: Additional file 1: Figure S1. Obtained dimensions of the 3D printed poly (lactic acid) (PLA) test device for various printing temperatures. Remaining parameters were kept constant at v = 30 mm/s, h = 50 µm and fan = 100%. Results are shown for the X-, Y- and Z-axis separately. Values shown as mean ± standard deviation of 3 devices. Figure S2. Obtained dimensions of the 3D printed poly (methyl methacrylate) (PMMA) test device for various printing temperatures. Remaining parameters were kept constant at v = 50 mm/s, h = 100 µm and fan = 50%. Results are shown for the X-, Y- and Z-axis separately. Values shown as mean ± standard deviation of 3 devices. Figure S3. Obtained dimensions of the 3D printed polycarbonate (PC) test device for various printing temperatures. Remaining parameters were kept constant at v = 50 mm/s, h = 100 µm and fan = 0%. Results are shown for the X-, Y- and Z-axis separately. Values shown as mean ± standard deviation of 3 devices. Figure S4. Obtained dimensions of the 3D printed poly (lactic acid) (PLA) test device for various printing speeds. Remaining parameters were kept constant at ϑ = 190 °C, h = 50 µm and fan = 100%. Results are shown for the X-, Y- and Z-axis separately. Values shown as mean ± standard deviation of 3 devices. Figure S5. Obtained dimensions of the 3D printed poly (methyl methacrylate) (PMMA) test device for various printing speeds. Remaining parameters were kept constant at ϑ = 245 °C, h = 100 µm and fan = 50%. Results are shown for the X-, Y- and Z-axis separately. Values shown as mean ± standard deviation of 3 devices. Figure S6. Obtained dimensions of the 3D printed polycarbonate (PC) test device for various printing speeds. Remaining parameters were kept constant at ϑ = 240 °C, h = 100 µm and fan = 0%. Results are shown for the X-, Y- and Z-axis separately Values shown as mean ± standard deviation of 3 devices. Figure S7. Obtained dimensions of the 3D printed poly (lactic acid) (PLA) test device for various layer heights. Remaining parameters were kept constant at ϑ = 190 °C, v =70mm/sand fan = 100%. Results are shown for the X-, Y- and Z-axis separately. Values shown as mean ± standard deviation of 3 devices. Figure S8. Obtained dimensions of the 3D printed poly (methyl methacrylate) (PMMA) test device for various layer heights. Remaining parameters were kept constant at ϑ = 245 °C, v = 70 mm/s and fan = 50%. Results are shown for the X-, Y- and Z-axis separately. Values shown as mean ± standard deviation of 3 devices. Figure S9. Obtained dimensions of the 3D printed polycarbonate (PC) test device for various layer heights. Remaining parameters were kept constant at ϑ = 240 °C, v = 80 mm/s and fan = 0%. Results are shown for the X-, Y- and Z-axis separately. Values shown as mean ± standard deviation of 3 devices. Figure S10. Obtained dimensions of the 3D printed poly (lactic acid) (PLA) test device for various fan speeds. Remaining parameters were kept constant at ϑ = 190 °C, v = 70 mm/s and h = 100 µm. Results are shown for the X-, Y- and Z-axis separately. Values shown as mean ± standard deviation of 3 devices. Figure S11. Obtained dimensions of the 3D printed poly (methyl methacrylate) (PMMA) test device for various fan speeds. Remaining parameters were kept constant at ϑ = 245 °C, v = 70 mm/s and h = 100 µm. Results are shown for the X-, Y- and Z-axis separately Values shown as mean ± standard deviation of 3 devices. Figure S12. Obtained dimensions of the 3D printed polycarbonate (PC) test device for various fan speeds. Remaining parameters were kept constant at ϑ = 240 °C, v = 80 mm/s and h = 100 µm. Results are shown for the X-, Y- and Z-axis separately. Values shown as mean ± standard deviation of 3 devices. Figure S13. Representative microscopic images of FDM printed micro channels in a poly (lactic acid) (PLA) test device. Analysis performed with “imageJ” (Version 1.52a, National Institutes of Health, USA). Scale bar measures 200 µm. Figure S14. Representative microscopic images of FDM printed micro channels in a poly (methyl methacrylate) (PMMA) test device. Analysis performed with “imageJ” (Version 1.52a, National Institutes of Health, USA). Scale bar measures 200 µm. Figure S15. Representative microscopic images of FDM printed micro channels in a PC test device. Analysis performed with “imageJ” (Version 1.52a, National Institutes of Health, USA). Scale bar measures 200 µm. Figure S16. Relative deviation between experimental dimensions and CAD dimensions in X-direction. Figure S17. Relative deviation between experimental dimensions and CAD dimensions in X-direction. Figure S18. Relative deviation between experimental dimensions and CAD dimensions in X-direction. Figure S19. Representative microscopic images of FDM printed polymer discs cultivated with human mesenchymal stem cells (hMSC) for 24 and 48 hours. Viability of hMSC cultivated on PLA, PMMA and PC discs was analyzed with MTT assay and compared to untreated hMSCs cultivated in a standard tissue culture 24-well plate. Images were taken after staining with MTT. Scale bar measures 500 µm.