Bradyrhizobium acaciae Avontuur &amp; Palmer &amp; Beukes &amp; Chan &amp; Tasiya &amp; Zyl &amp; Coetzee &amp; Stepkowski &amp; Venter &amp; Steenkamp 2022, sp. nov.
Creators: name: Avontuur, Juanita R.
name: Palmer, Marike
name: Beukes, Chrizelle W.
name: Chan, Wai Y.
name: Tasiya, Taponeswa
name: Zyl, Elritha van
name: Coetzee, Martin P. A.
name: Stepkowski, Tomasz
name: Venter, Stephanus N.
name: Steenkamp, Emma T.
Publisher: name: Zenodo
Year: 2021
Subjects: Biodiversity
Taxonomy
Bacteria
Proteobacteria
Alphaproteobacteria
Rhizobiales
Bradyrhizobiaceae
Bradyrhizobium
Bradyrhizobium acaciae

description: <i>4.1. Description of Bradyrhizobium acaciae sp. nov.</i> <i>Bradyrhizobium acaciae</i> (a.ca’ ci.ae. N.L. fem. gen. n. <i>acaciae</i>, referring to the host plant genus <i>Acacia</i>, from which the species was isolated). Cells are Gram-negative, motile, rod-shaped and approximately 2.35 × 0.65 μm in size. Colonies whengrownon YMA at 28 ◦ C after 7 daysare convex and can either be translucent or opaque, cream in colour and less than 1 mm in diameter. Isolates within this species can grow in temperatures ranging from 10 to 40 ◦ C and at a pH between 5 and 10 and cannot grow in the presence of more than 1.5% NaCl (w/v). They are positive for oxidase, urease, β- glucosidase and β- galactosidase activity. Positive reactions were recorded for the following carbon utilization: Larabinose, D-mannose, potassium gluconate, Tween 40, L-asparagine, Tween 80, L-frucotose, D-galactose, D-galacturonic acid, α- D-glucose, Larabinose, L-rhamnose, formic acid, D-saccharic acid, glucuronamide, D- and L- alanine, and L-aspartic acid. Carbon utilization reactions was negative for L-tryptophan, glucose fermentation, hydrolysis of gelatin, D-mannitol, D-maltose, i-erythritol, gentibiose, m-inositol, D-melibiose, L-ornithine, capric acid, adipic acid, citrate utilization, phenylacetic acid, malic acid, α- cyclodextrin, glycogen, N-acetyl-D-galactosamine, glucosamine, D-raffinose, adonitol, D-arabitol, D-cellobiose, D-glucoside, D-psicose, sucrose, D-trehalose, xylitol, D-sorbitol, D-glucoronic acid, glucose-1-phosphate and glucose-6-phosphate. Resistance to ampicillin (10 μg/ml), penicillin (10 μg/ml), erythromycin (30 μg/ml), streptomycin (25 μg/ml), tetracycline (30 μg/ml), neomycin (10 μg/ml), chloramphenicol (30 μg/ml), kanamycin (30 μg/ml) and susceptible to gentamycin (10 μg/ml) and streptomycin (10 μg/ml). The type strain, 10 BBT (=SARCC 730 = LMG 31409), was isolated from nodules of <i>Acacia dealbata</i> growing in the Gauteng Province of South Africa and can form effective nodules on cowpea (<i>Vigna unguiculata</i>) and siratro (<i>Macroptilium atropurpureum</i>). The DNA G + Ccontent of the type strain is 63.6 mol%. GenBank/EMBL/DDBJ accession numbers for the genome sequence and gene sequences of this type strain are: whole genome sequence (JACMYK000000000), 16S rRNA (LR877293), <i>atpD</i> (LR877274), <i>glnII</i> (LR877284), <i>dnaK</i> (LR877288), <i>gyrB</i> (LR877279), <i>rpoB</i> (LR877266) and <i>nodA</i> (LR880919).
description: Published as part of <i>Avontuur, Juanita R., Palmer, Marike, Beukes, Chrizelle W., Chan, Wai Y., Tasiya, Taponeswa, Zyl, Elritha van, Coetzee, Martin P. A., Stepkowski, Tomasz, Venter, Stephanus N. &amp; Steenkamp, Emma T., 2022, Bradyrhizobium altum sp. nov., Bradyrhizobium oropedii sp. nov. and Bradyrhizobium acaciae sp. nov. from South Africa show locally restricted and pantropical nodA phylogeographic patterns, pp. 1-13 in Molecular Phylogenetics and Evolution (107338) (107338) 167</i> on page 10, DOI: 10.1016/j.ympev.2021.107338, http://zenodo.org/record/5728354