Additional file 1: Table S1. of Circulating proteins in response to combined-modality therapy in rectal cancer identified by antibody array screening
Creators: name: Erta Kalanxhi
name: Hektoen, Helga
name: Meltzer, Sebastian
name: Dueland, Svein
name: Flatmark, Kjersti
name: Ree, Anne
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biochemistry
subject: Space Science
subject: Medicine
subject: Cell Biology
subject: Biotechnology
subject: Immunology
subject: FOS: Clinical medicine
subject: FOS: Clinical medicine
subject: Cancer
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: FOS: Health sciences
subject: 60506 Virology
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Computational Biology

description: Numbers of serum samples from study patients. The initial population of 66 study patients had serum sampled at baseline, following induction neoadjuvant chemotherapy (post-NACT) and sequential chemoradiotherapy (post-CRT), and at evaluation of the neoadjuvant treatment. Applying Common Terminology Criteria for Adverse Events (CTCAE) version 3.0 and omitting cases reporting CTCAE grade &gt;0 diarrhea at baseline or from whom definite maximum CTCAE score was not available, the initial number of 53 study patients remained for the analysis of treatment toxicity (maximum diarrhea CTCAE score) during the neoadjuvant treatment course. Missing values as a result of processing of the antibody array data from serum samples led to reduction in the number of cases with lipocalin-2 (LCN2) and matrix metalloproteinase-9 (MMP9) measurements and subsequently, the number of cases with matched values for fold-change from baseline ratios available for down-stream analyses. Table S2. Association between altered serum values (array fluorescence intensities as fold-change from baseline) of the four listed proteins (identities given by the gene names) at completion of induction neoadjuvant chemotherapy (post-NACT) and sequential chemoradiotherapy (post-CRT), entered as continuous data from lowest to highest measurements, and PFS was modeled by Cox regression analysis and given as adjusted hazard ratio (HR) with 95% confidence interval (CI); HR above 1: higher probability of unfavorable PFS for the higher value(s) of the parameter. In neither case, altered array values at completion of NACT or CRT were associated with PFS. Table S3. Study patients’ characteristics. The initial population of 66 study patients had serum sampled at baseline, following induction neoadjuvant chemotherapy (post-NACT) and sequential chemoradiotherapy (post-CRT), and at evaluation of the neoadjuvant treatment. One patient had disease progression in the pelvic cavity during the neoadjuvant treatment and therefore proceeded to palliative surgery. As a consequence, histologic tumor response data (ypTN stage and tumor regression grade (TRG) scoring) were missing. The serum samples were analyzed with an antibody array. Array data processing led to reduction in the number of cases with lipocalin-2 (LCN2) and matrix metalloproteinase-9 (MMP9) measurements and subsequently, the number of cases with matched values for fold-change from baseline ratios – given here – that were available for down-stream analyses. Distribution of parameters was analyzed using Pearson product correlation for continuous data and paired t-test for categorical variables. Within the total study population as well as all of the LCN2 and MMP9 groups of study cases, patients’ characteristics were equally distributed. Table S4. Treatment-induced enteropathy in study patients. Using one-way analysis of ranks, altered serum values (array fluorescence intensities as fold-change from baseline) of lipocalin-2 (LCN2) and matrix metalloproteinase-9 (MMP9) following induction neoadjuvant chemotherapy (post-NACT) and sequential chemoradiotherapy (post-CRT) were compared for patients categorized into four groups of maximum diarrhea scores, as assessed by Common Terminology Criteria for Adverse Events (CTCAE) version 3.0, during the neoadjuvant treatment course. Categories are Group 0: CTCAE grade 0 diarrhea; Group 1: CTCAE grade 1 diarrhea; Group 2: CTCAE grade 2 diarrhea; Group 3: CTCAE grade 3 diarrhea. (DOC 99 kb)