Additional file 1 of Highly selective synthesis of d-amino acids via stereoinversion of corresponding counterpart by an in vivo cascade cell factory
Creators: name: Zhang, Dan-Ping
name: Jing, Xiao-Ran
name: Wu, Lun-Jie
name: Fan, An-Wen
name: Nie, Yao
name: Xu, Yan
Publisher: name: figshare
Year: 2021
Subjects: subject: Biochemistry
subject: Space Science
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Genetics
subject: Molecular Biology
subject: Chemical Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Infectious Diseases
subject: FOS: Health sciences

description: Additional file 1: Table S1. Primers used in this study. Fig. S1. SDS-PAGE analysis of E. coli pET-21b-MBP-laad/pET-28a-dapdh-fdh induced by different concentrations of IPTG. T: total cell lysate; S: soluble fraction. The samples were generated from 50 mg/mL of E. coli pET-21b-MBP-laad/pET-28a-dapdh-fdh cells. Fig. S2. SDS-PAGE analysis of E. coli pET-21b-laad/pET-28a-dapdh-fdh induced by different concentrations of IPTG. T: total cell lysate; S: soluble fraction. The samples were generated from 50 mg/mL of E. coli pET-21b-laad/pET-28a-dapdh-fdh cells. Fig. S3. SDS-PAGE analysis of E. coli pET-28a-dapdh-fdh-laad induced by different concentrations of IPTG. T: total cell lysate; S: soluble fraction. The samples were generated from 50 mg/mL of E. colii pET-28a-dapdh-fdh-laad cells. Fig. S4. Time course of d-Phe production from diferent concentration of l-Phe. A: The concentration of l-Phe was 100 mM; B: The concentration of l-Phe was 150 mM. The concentration of E. coli pET-21b-MBP-laad/pET-28a-dapdh-fdh whole-cell biocatalyst was 75 mg/mL wet cell, the dosage of NADP+ was 0.1 time of substrate concentration, the dosage of NH4Cl was 4 times of the substrate concentration, and the dosage of sodium formate was 3 times of substrate concentration. All the reactions were carried out in Tris-HCl buffer (50 mM, pH 9.0) at 30 ℃ and 220 rpm. Fig. S5. HPLC analysis of l-Phe and d-Phe. Retention time of l-Phe is 34.620 min; retention time of d-Phe is 41.287 min. Fig. S6. HPLC analysis of l-leucine and d-leucine. Retention time of l-leucine is 32.313 min; retention time of d-leucine is 36.100 min. Fig. S7. HPLC analysis of l-glutamic acid and d-glutamic acid. Retention time of l-glutamic acid is 18.760 min; retention time of d-glutamic acid is 20.327 min. Fig. S8. HPLC analysis of l-lysine and d-lysine. Retention time of l-lysine is 28.267 min; retention time of d-lysine is 34.46 min. Fig. S9. HPLC analysis of l-methionine and d-methionine. Retention time of l-methionine is 28.54 min; retention time of d-methionine is 31.92 min. Fig. S10. HPLC analysis of l-norvaline and d-norvaline. Retention time of l-norvaline is 29.153 min; retention time of d-norvaline is 33.120 min. Fig. S11. HPLC analysis of l-tyrosine and D-tyrosine. Retention time of l-tyrosine is 37.067 min; retention time of d-tyrosine is 40.067 min. Fig. S12. HPLC analysis of l-tryptophan and D-tryptophan. Retention time of l-tryptophan is 30.773 min; retention time of l-tryptophan is 33.153 min. Fig. S13. HPLC analysis of l-homophenyalanine and d-homophenyalanine. Retention time of l-homophenyalanine is 35.04 min; retention time of d-homophenyalanine is 38.713 min. Fig. S14. HPLC analysis of 2-chloro-l-phenylalanine and 2-chloro-d-phenylalanine. Retention time of 2-chloro-l-phenylalanine is 37.66 min; retention time of 2-chloro-d-phenylalanine is 34.227 min. Fig. S15. HPLC analysis of 3-chloro-l-phenylalanine and 3-chloro-d-phenylalanine. Retention time of 3-chloro-l-phenylalanine is 35.473 min; retention time of 3-chloro-d-phenylalanine is 38.713 min. Fig. S16. HPLC analysis of 4-chloro-l-phenylalanine and 4-chloro-d-phenylalanine. Retention time of 4-chloro-l-phenylalanine is 36.12 min; retention time of 4-chloro-d-phenylalanine is 39.42 min. Fig. S17. HPLC analysis of 4-methyl-l-phenylalanine and 4-methyl-d-phenylalanine. Retention time of 4-methyl-l-phenylalanine is 35.347 min; retention time of 4-methyl-d-phenylalanine is 38.600 min.