cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Additional file 9: Figure S7. of A global analysis of the complex landscape of isoforms and regulatory networks of p63 in human cells and tissues
Creators: name: Isha Sethi
name: Rose-Anne Romano
name: Gluck, Christian
name: Smalley, Kirsten
name: Vojtesek, Borivoj
name: Buck, Michael
name: Satrajit Sinha
Publisher: name: Figshare
Year: 2015
Subjects: subject: Biochemistry
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Cancer
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Unsupervised Clustering of the human cell-lines. The 40 cell-types (54 experiments) clustered using unsupervised hierarchical clustering based on average linkage and Pearson correlation distance metric. TAp63 expressing cell-lines are shown in green, Î Np63 expressing cell-lines are shown in red. rep1: replicate 1, rep2: replicate 2. (PDF 30 kb)
Antiochtha leucograpta Onebala
Creators: name: Das, Apurva
name: Pathania, Prakash C.
name: Singh, Navneet
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Lepidoptera
Lecithoceridae
Antiochtha
Antiochtha leucograpta

description: 115. Antiochtha leucograpta (Meyrick, 1923 c: 45) (Onebala)
Type locality: India, Tamil Nadu, Ootacamund
Distribution: Indian records: Tamil Nadu. Global records: China.
description: Published as part of Das, Apurva, Pathania, Prakash C. & Singh, Navneet, 2022, A catalogue of Indian Lecithoceridae (Lepidoptera: Gelechioidea), pp. 56-78 in Zootaxa 5165 (1) on page 70, DOI: 10.11646/zootaxa.5165.1.2, http://zenodo.org/record/6825526
Wyushinamia bifurcata , Zhang and Duan 2006, sp. nov.
Creators: name: Zhang, Yalin
name: Duan, Yani
Publisher: name: Zenodo
Year: 2006
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hemiptera
Cicadellidae
Wyushinamia
Wyushinamia bifurcata

description: Wyushinamia bifurcata Zhang and Duan sp. nov.
Figs. 9–16
Vertex light yellow with six small dark brown marks on anterior margin, and brown longitudinal band midway between midline and eye extending to posterior margin of pronotum; eyes dark, ocelli light yellow; clypeus with fuscous arcs. Pronotum light yellow with six brown longitudinal stripes including the two middle ones from vertex. Scutellum stramineous. Legs marked with brown. Forewings stramineous or brown, cells bordered with brown, veins paler.
Vertex convex, nearly as long as next to eye, ocellus separated from eye by less than ocellus’ diameter; apex of clypellus tapered. Pronotum nearly as long as vertex. Scutellum slightly shorter than vertex. Forewing macropterous, outer anteapical cell more than 1/2 central anteapical cell, central anteapical cell constricted, inner anteapical cell open basally, veins conspicuous, discal cell near costal area and with a cross vein.
Male genitalia: Pygofer constricted distally. Subgenital plate with lateral margin concave. Valve with anterior margin arched, posterior margin triangularly produced medially. Aedeagal shaft slender, longer than twice length of connective, apex bifurcate with a toothlike protrusion at inside of each branch, aedeagal shaft sinuate in lateral aspect, gonopore apical on ventral surface.
Measurement Male Length (including tegmen): 4.1mm.
Material examined
Holotype ♂, China: Fujian Prov., Mt. Wuyishan, Xianfengling, 21 July 2003, coll. Duan Yani (NWAFU). Paratypes: 2 ♂, 1 ♀, China, Fujian Prov., Mt. Wuyishan, Pikeng, 20 July 2006, coll. Yang Meixia.
Remarks
Externally this species resembles some species of Deltocephalus but differs in having more elongate male pygofer and subgenital plates, and the aedeagal shaft apex bifurcate with a toothlike protuberance at inside of each branch.
Etymology The species name is based on the apically bifurcate aedeagal shaft.
description: Published as part of Zhang, Yalin & Duan, Yani, 2006, Two new Deltocephalini leafhopper genera (Hemiptera: Cicadellidae: Deltocephalinae) from China, pp. 51-56 in Zootaxa 1332 (1) on pages 54-56, DOI: 10.11646/zootaxa.1332.1.3, http://zenodo.org/record/5071228
Psychosocial stress induction in vivo vs. in virtuo and the influence of a health app on the acute stress reaction in youths: a study protocol for a randomized controlled trial
Creators: name: Schleicher, Daniel
name: Ecker, Angelika
name: Kocur, Martin
name: Jarvers, Irina
name: Nash, Colin
name: Götz, Leonie
name: Otto, Alexandra H.
name: Kandsperger, Stephanie
name: Brunner, Romuald
Publisher: name: Universität Regensburg
Year: 2022
Subjects: subject: EARLY-LIFE STRESS; CORTISOL RESPONSES; SALIVARY CORTISOL; QUESTIONNAIRE; CHILDREN; VALIDATION; INVENTORY; MANAGEMENT; ENDOCRINE; VERSION; Trier Social Stress Test; Virtual reality; Ambulatory Assessment; Cortisol; Stress mechanisms; Coping; Children and adolescents; Relaxation; Eye-tracking
subject: 004 Informatik
subject: 610 Medizin

description: Background Acute and everyday stress is substantial for the development of mental and physical diseases, therefore it is crucial to get a better understanding of its pathogenesis. Different methods (e.g., Ambulatory Assessment) and stress reactivity paradigms (e.g., Trier Social Stress Test / TSST) in laboratory settings are often used to investigate basic mechanisms of this process. Due to the technological progress of the last years and especially due to children and adolescents growing up with it, the application of these developments in clinical research is reasonable. The aim of this project is to successfully transfer the TSST for children and adolescents into the virtual world, which will be compared to a real TSST situation. Physiological and psychological stress reactions will be analyzed in order to assess similarities and differences. Moreover, it will be investigated whether a Heart Coherence Exercise (HCE) has a stronger influence on coping with acute stress compared to Natural Relaxation (NR). Methods This single-center experimental study will examine acute and everyday stress and coping processes in eighty-four healthy children and adolescents between the ages of 11 and 17. For everyday stress, different parameters (e.g., hormonal profiles and mood ratings) as well as a history of stressful life events and utilized coping methods will be recorded and a relaxation exercise will be practiced on a smartphone over 2 days. Regarding the acute stress reaction, the participants will be confronted either with the virtual or the real version of the TSST, followed by the trained relaxation exercise (HCE vs. NR). Physiological (e.g., cortisol and heart rate) and psychological stress markers (e.g., mood and gaze behavior) will be recorded continuously. Discussion Studies are sparse using a virtual version of the TSST in children and adolescents. A successful virtual TSST would constitute an economical variant, which would also make it easier to administer it in clinical or population-based samples. Effective ambulatory relaxation exercises would be a useful addition to clinical treatment approaches.
Use and acceptance of traditional, complementary and integrative medicine in Germany—an online representative cross-sectional study
Creators: name: Jeitler, Michael
name: Ortiz, Miriam
name: Brinkhaus, Benno
name: Sigl, Mike
name: Hoffmann, Rasmus
name: Trübner, Miriam
name: Michalsen, Andreas
name: Wischnewsky, Manfred
name: Kessler, Christian
Publisher: name: Humboldt-Universität zu Berlin
Year: 2024
Subjects: subject: traditional medicine
subject: traditional European medicine
subject: complementary medicine
subject: integrative medicine
subject: alternative medicine
subject: online-representative
subject: cross-sectional study
subject: Naturheilkunde
subject: 610 Medizin und Gesundheit

description: Background: Older representative surveys show that Traditional, Complementary and Integrative Medicine (TCIM) is used by about 60% of the German population. However, no data exists for the current nationwide situation. The main aim of this cross-sectional study is to investigate the current use and acceptance of TCIM in Germany. Methods: This study is based on a representative sample of the German population aged 18–75 years. Participants were asked about the use and acceptance of TCIM. The survey was conducted online using Computer Assisted Web Interview (CAWI) in 2022 by three renowned German market research institutes on behalf of and in close coordination with the working group. The data set was analyzed descriptively and inferentially. Results: In total, 4,065 participants (52% female, 48% male, 0.4% diverse) responded completely (response rate: 21.5%). Among participants, 70% stated that they had used TCIM at some point in their lives, with 32% doing so in the last 12 months and 18% currently. The most common reason given (17%) was musculoskeletal pain. For their own health, 39% stated that TCIM is important. Traditional European Medicine was rated as very/mainly effective by 27% of participants and as partly effective by 44% (conventional medicine: 69% very/mainly effective, 19% partly effective). As a complementary treatment strategy to conventional medicine, 35% considered TCIM to be optimal (“Complementary Medicine”), 33% in combination with conventional medicine (“Integrative Medicine”) and 5% without conventional medicine (“Alternative Medicine”). The majority of the participants were in favor of more research on TCIM and stated that the costs of TCIM services should be covered by health insurance companies (71% and 69%, respectively). Conclusion: These results from a representative online-population suggest that the use of TCIM in Germany remains at a high level. The nationwide relevance of TCIM should be given greater consideration in German health care policy making. TCIM should be systematically investigated using appropriate study designs and methods including high quality randomized clinical trials to investigate their effectiveness, efficacy, therapeutic safety and costs in the future.
Expanding the genetic toolbox for the obligate human pathogen Streptococcus pyogenes
Creators: name: Lautenschläger, Nina
name: Schmidt, Katja
name: Schiffer, Carolin
name: Wulff, Thomas Fabian
name: Hahnke, Karin
name: Finstermeier, Knut
name: Mansour, Moïse
name: Elsholz, Alexander K. W.
name: Charpentier, Emmanuelle
Publisher: name: Humboldt-Universität zu Berlin
Year: 2024
Subjects: subject: Streptococcus pyogenes
subject: plasmid collection
subject: inducible promoter
subject: genetic engineering
subject: reporter gene
subject: scarless gene deletion
subject: synthetic biology
subject: 570 Biologie

description: Genetic tools form the basis for the study of molecular mechanisms. Despite many recent advances in the field of genetic engineering in bacteria, genetic toolsets remain scarce for non-model organisms, such as the obligatory human pathogen Streptococcus pyogenes. To overcome this limitation and enable the straightforward investigation of gene functions in S. pyogenes, we have developed a comprehensive genetic toolset. By adapting and combining different tools previously applied in other Gram-positive bacteria, we have created new replicative and integrative plasmids for gene expression and genetic manipulation, constitutive and inducible promoters as well as fluorescence reporters for S. pyogenes. The new replicative plasmids feature low- and high-copy replicons combined with different resistance cassettes and a standardized multiple cloning site for rapid cloning procedures. We designed site-specific integrative plasmids and verified their integration by nanopore sequencing. To minimize the effect of plasmid integration on bacterial physiology, we screened publicly available RNA-sequencing datasets for transcriptionally silent sites. We validated this approach by designing the integrative plasmid pSpy0K6 targeting the transcriptionally silent gene SPy_1078. Analysis of the activity of different constitutive promoters indicated a wide variety of strengths, with the lactococcal promoter P23 showing the strongest activity and the synthetic promoter PxylS2 showing the weakest activity. Further, we assessed the functionality of three inducible regulatory elements including a zinc- and an IPTG-inducible promoter as well as an erythromycin-inducible riboswitch that showed low-to-no background expression and high inducibility. Additionally, we demonstrated the applicability of two codon-optimized fluorescent proteins, mNeongreen and mKate2, as reporters in S. pyogenes. We therefore adapted the chemically defined medium called RPMI4Spy that showed reduced autofluorescence and enabled efficient signal detection in plate reader assays and fluorescence microscopy. Finally, we developed a plasmid-based system for genome engineering in S. pyogenes featuring the counterselection marker pheS*, which enabled the scarless deletion of the sagB gene. This new toolbox simplifies previously laborious genetic manipulation procedures and lays the foundation for new methodologies to study gene functions in S. pyogenes, leading to a better understanding of its virulence mechanisms and physiology.
Paraclevelandia brevis Kidder 1937
Creators: name: Kotyk, Michael
name: Bourland, William A.
name: Soviš, Matyáš
name: Méndez-Sánchez, Daniel
name: Škaloud, Pavel
name: Varadínová, Zuzana Kotyková
name: Čepička, Ivan
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Chromista
Ciliophora
Clevelandellidae
Paraclevelandia
Paraclevelandia brevis

description: Paraclevelandia brevis in ‘simplex’ form
(Figs 23H–P, 24C, F; Supporting Information, Tables S30–S33)
When present, the simplex form was present in low to moderate abundance.
Description based on ASS, PAA and STH in vivo,andPAA, STH, and SRU in protargol preparations: Medium-sized Clevelandellidae (on average 84 × 47 µm in vivo, range 70–101 × 38–58 µm; on average 76 × 45 in protargol preparations, range 61–98 × 37–53 µm). Ovate to almost broadly fusiform, anterior end tapers to rounded point, posterior end broadly rounded, peristomial projection absent as in ‘brevis’ form; distinct left lobe absent. Macronucleus elongated, cylindrical, situated longitudinally in anterior half of cell near right cell margin. Micronucleus oval, near midportion of macronucleus, smaller compared to brevis form (3.4 vs. 5.3 µm). A sack-like structure formed between anterior margin of macronucleus and anterior end of cell as reported by Kidder (1937, 1938), visible both in vivo (Fig. 23H, I) and protargol preparations (Fig. 23J). Karyophore inconspicuous, not visible in vivo, sometimes visible in protargol preparations (Fig. 23J, O), attached to both anterior and posterior end of macronucleus. Cortex with refractile interkinetal granules, probably mucocysts (Fig. 23I). Somatic ciliature in same pattern and distribution as ‘brevis’ form. Kinetal furrows conspicuous. Up to 20 free kinetofragments in right suture (Fig. 23K, P). Organization of oral ciliature as for the family with same exceptions as ‘brevis’ form. Adoral zone narrows from posterior to anterior, extends about 50% of cell length on average, composed of an average of 28 membranelles (range 25–32).
description: Published as part of Kotyk, Michael, Bourland, William A., Soviš, Matyáš, Méndez-Sánchez, Daniel, Škaloud, Pavel, Varadínová, Zuzana Kotyková & Čepička, Ivan, 2024, Morphology maưers: congruence of morphology and phylogeny in the integrative taxonomy of Clevelandellidae (Ciliophora: Armophorea) with description of six new species, pp. 1-51 in Zoological Journal of the Linnean Society 202 (1) on page 24, DOI: 10.1093/zoolinnean/zlad154, http://zenodo.org/record/14541981
Antiochtha trigonopis Tiriza
Creators: name: Das, Apurva
name: Pathania, Prakash C.
name: Singh, Navneet
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Lepidoptera
Lecithoceridae
Antiochtha
Antiochtha trigonopis

description: 116. Antiochtha trigonopis (Meyrick, 1907: 737) (Tiriza)
Type locality: India, Himachal Pradesh, Simla [Shimla]
Distribution: Indian records: Himachal Pradesh.
description: Published as part of Das, Apurva, Pathania, Prakash C. & Singh, Navneet, 2022, A catalogue of Indian Lecithoceridae (Lepidoptera: Gelechioidea), pp. 56-78 in Zootaxa 5165 (1) on page 70, DOI: 10.11646/zootaxa.5165.1.2, http://zenodo.org/record/6825526
Workshop zum Forschungsdatenmanagement an HAWs / FHs: Austausch über Strategien, Herausforderungen und Lösungsansätze
Creators: name: Schneidenbach, Esther
name: Herrmann, Karoline
name: Schmidt, Diana
name: Heister, Eske
Publisher: name: Zenodo
Year: 2024

description: Workshop-Dokumentation des "Workshops zum Forschungsdatenmanagement an HAWs / FHs: Austausch über Strategien, Herausforderungen und Lösungsansätze" auf der FORTRAMA Jahrestagung in Regensburg am 12.03.2024
Aproparia Gozmany 1972
Creators: name: Das, Apurva
name: Pathania, Prakash C.
name: Singh, Navneet
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Lepidoptera
Lecithoceridae
Aproparia

description: Genus Aproparia Gozmány, 1972: 291
Type species: Onebala pselaphistis Meyrick, 1910b, by original designation
description: Published as part of Das, Apurva, Pathania, Prakash C. & Singh, Navneet, 2022, A catalogue of Indian Lecithoceridae (Lepidoptera: Gelechioidea), pp. 56-78 in Zootaxa 5165 (1) on page 71, DOI: 10.11646/zootaxa.5165.1.2, http://zenodo.org/record/6825526
Additional file 3: Figure S1. of Molecular Pap smear: HPV genotype and DNA methylation of ADCY8, CDH8, and ZNF582 as an integrated biomarker for high-grade cervical cytology
Creators: name: Shen-Gunther, Jane
name: Chiou-Miin Wang
name: Poage, Graham
name: Lin, Chun-Lin
name: Perez, Luis
name: Banks, Nancy
name: Huang, Tim
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biochemistry
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: 69999 Biological Sciences not elsewhere classified
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics
subject: Developmental Biology
subject: Cancer
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: 60506 Virology
subject: Computational Biology

description: DNA methylation and gene expression of ADCY8, CDH8, ZNF582, and MGMT in the TCGA cervical cancer cohort. (PDF 106 kb)
Quantum-limited superresolution of two arbitrary incoherent point sources: beating the resurgence of Rayleigh's curse
Creators: name: Li, Junyan
name: Pang, Shengshi
Publisher: name: arXiv
Year: 2024
Subjects: subject: Quantum Physics (quant-ph)
subject: FOS: Physical sciences

description: Abstract Superresolution has been demonstrated to overcome the limitation of the Rayleigh's criterion and achieve significant improvement of the precision in resolving the separation of two incoherent optical point sources. However, in recent years, it was found that if the photon numbers of the two incoherent optical sources are unknown, the precision of superresolution vanishes when the two photon numbers are actually different. In this work, we first analyze the estimation precision of the separation between two incoherent optical sources with the same point-spread functions in detail, and show that when the photon numbers of the two optical sources are different but sufficiently close, the superresolution can still realized but with different precisions. We find the condition on how close the photon numbers of two optical sources need to be to realize the superresolution, and derive the precision of superresolution in different regimes of the photon number difference. We further consider the superresolution for two incoherent optical sources with different point-spread functions, and show that the competition between the difference of photon numbers, the difference of the two point-spread functions and the separation of the two optical sources determines the precision of superresolution. The results exhibit precision limits distinct from the case of two point sources with identical point-spread functions and equal photon numbers, and extend the realizable regimes of the quantum superresolution technique. The results are finally illustrated by Gaussian point-spread functions.
description: v2: Close to the published version
Aproparia pselaphistis Onebala
Creators: name: Das, Apurva
name: Pathania, Prakash C.
name: Singh, Navneet
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Lepidoptera
Lecithoceridae
Aproparia
Aproparia pselaphistis

description: 117. Aproparia pselaphistis (Meyrick, 1910b: 456) (Onebala)
Type locality: India, Assam, Khasis [Now in Meghalaya]
Distribution: Indian records: Meghalaya.
description: Published as part of Das, Apurva, Pathania, Prakash C. & Singh, Navneet, 2022, A catalogue of Indian Lecithoceridae (Lepidoptera: Gelechioidea), pp. 56-78 in Zootaxa 5165 (1) on page 71, DOI: 10.11646/zootaxa.5165.1.2, http://zenodo.org/record/6825526
Clevelandella parapanesthiae
Creators: name: Kotyk, Michael
name: Bourland, William A.
name: Soviš, Matyáš
name: Méndez-Sánchez, Daniel
name: Škaloud, Pavel
name: Varadínová, Zuzana Kotyková
name: Čepička, Ivan
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Chromista
Ciliophora
Heterotrichea
Heterotrichida
Clevelandellidae
Clevelandella
Clevelandella parapanesthiae

description: Clevelandella parapanesthiae (Kidder, 1937)
(Figs 28, 29; Supporting Information, Tables S35–S40) Four populations (ASS, PGW, PAC, and MP) were morphologically characterized by both in vivo observations and in protargol preparations and two (PT and STH) only in vivo. The ciliate was moderately to highly abundant.
Description based on ASS, PGW, PAC, and MP populations: Medium-sized Clevelandella (on average 99 × 55 µm in vivo, range 66–119 × 31–71 µm; on average 86 × 46 µm in protargol preparations, range 70–114 × 33–56 µm). Spade-shaped with distinct left notch and protrusive left lobe; peristomial projection short, broad, about 24% of body length. Macronucleus from slender inverted teardrop-shape (Fig. 28A, G, H) to scimitar-like (Fig. 28C, E, F), obliquely oriented, situated right of midline, posterior end extends into posterior part of body proper, karyophore at posterior end of macronucleus, visible only inconsistently in vivo and in protargol preparations. In MP population cells with S-like (Fig. 28B) macronucleus (as well as normal teardrop-shaped) were observed. Micronucleus ellipsoidal, inconspicuous. Somatic cilia limited to approximately anterior 67% of cell. About 10 obliquely oriented, free right sutural kinetofragments in the right suture (Fig. 28D). Adoral zone extends about 56% of cell length on average, composed of an average of 38 membranelles (range 35–42). POM diplostichomonad only in anterior quarter (Fig. 28F).
description: Published as part of Kotyk, Michael, Bourland, William A., Soviš, Matyáš, Méndez-Sánchez, Daniel, Škaloud, Pavel, Varadínová, Zuzana Kotyková & Čepička, Ivan, 2024, Morphology maưers: congruence of morphology and phylogeny in the integrative taxonomy of Clevelandellidae (Ciliophora: Armophorea) with description of six new species, pp. 1-51 in Zoological Journal of the Linnean Society 202 (1) on page 30, DOI: 10.1093/zoolinnean/zlad154, http://zenodo.org/record/14541981
Multistep screening and selection of COVID‐19 convalescent plasma donors at the early stage of the SARS‐CoV‐2 pandemic: A retrospective analysis
Creators: name: Brosig, Andreas Michael
name: Ossner, Thomas
name: Pamler, Irene
name: Friedinger, Susanne
name: Bica, Adelina‐Florina
name: Mohrez, Morad
name: Tlili, Ikram
name: Mueller, Viktoria
name: Becke, Christine
name: Haehnel, Viola
name: Baeuerlein, Veronika
name: Stemmer, Barbara
name: Burkhardt, Ralph
name: Offner, Robert
Publisher: name: Universität Regensburg
Year: 2022
Subjects: subject: blood donation, convalescent plasma, COVID‐19, donor screening, SARS‐CoV‐2 pandemic
subject: 610 Medizin

description: Background and Aims
The COVID-19 pandemic reached Bavaria in February 2020. Almost simultaneously, Chinese physicians published reports on the first successful treatments with plasma from COVID-19 convalescent donors. With these silver linings on the horizon, we decided to establish the manufacturing of anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibody-containing plasma from COVID-19 convalescent donors at our site. Here we describe our donor selection process, built from the ground up, which enabled us to cope with the immense resonance after our social media call for donors.
Methods
As a first step, we created a specific questionnaire for telephone interviews applied by trained students to filter the wave of callers interested in plasma donation. Afterward, the medical staff evaluated the hotline questionnaires and chose eligible donors to be invited for on-site donor evaluation. Data documentation was performed with MS Excel, and statistical analyses were calculated with GraphPad Prism 8. A quantitative in-house ELISA was used to detect anti-SARS-CoV-2 antibodies and determine specific titers.
Results
Out of 1465 calls from potential plasma donors, we could register 420 persons with a completed questionnaire. Evaluation of questionnaires identified 222 of 420 persons as eligible for donation, and 55 were directly asked for on-site donor qualification. Subsequently, as anti-SARS-CoV-2 antibody titers ≥1:800 were required, we invited 89 of 222 potential donors for an antibody screening. This procedure resulted in another 28 potential donors for an on-site evaluation. Finally, 12 donors qualified with a titer of 1:400 and 24 with ≥1:800.
Conclusion
Identifying suitable COVID-19 convalescent plasma donors was expected to be highly time-consuming. Implementing a screening procedure with our hotline questionnaire helped us streamline the donor selection process and reduce the workload for the staff. We propose combining the described selection process with the later introduced on-site antibody screening as an effective strategy.
Additional file 2: Table S1. of Effects of Greenselect PhytosomeÂŽ on weight maintenance after weight loss in obese women: a randomized placebo-controlled study
Creators: name: Gilardini, Luisa
name: Pasqualinotto, Lucia
name: Pierro, Francesco Di
name: Risso, Paolo
name: Invitti, Cecilia
Publisher: name: Figshare
Year: 2016
Subjects: subject: Medicine
subject: Physiology
subject: FOS: Biological sciences
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics
subject: Developmental Biology
subject: Computational Biology

description: Dietary composition and physical activity of obese women of GSP and P group before and after the 3-month lifestyle intervention. (DOCX 16Â kb)
Praxiseinsatz Elektronischer Patientenakten: Erkenntnisse aus 2 Versorgungsprojekten in Zentren für Seltene Erkrankungen
Creators: name: Rashid, Asarnusch
name: Choukair, Daniela
name: Bauer, Christoph
name: Ullrich, Melanie
name: Maisch, Tim
Publisher: name: Universität Regensburg
Year: 2022
Subjects: subject: HEALTH-CARE; eHealth; Networks; Telemedicine; Teleconsultation; Digitization
subject: 610 Medizin

description: An electronic patient record offers opportunities for digital networks between medical care providers and for the digital communication between health service providers and their patients. Patients with rare diseases benefit from a diagnosis and treatment information at an early stage and receive precise treatment on the basis of multiprofessional case management. Regarding the patient care and medical research in rare diseases, electronic patient records can help to collect all data in a structured manner and to digitally map the workflows in registration, admission, diagnosis, and treatment. This can reduce costs in our healthcare system, as diagnosis and treatment can be targeted better at the patients and unnecessary medical examinations can be reduced. In two pilot projects, first experiences with electronic patient records for patients with rare diseases were gathered. In cooperation with several medical care providers, the projects BASE-Netz and TRANSLATE-NAMSE analyzed the requirements of an electronic patient record, demonstrated the technical and legal feasibility, and evaluated the practicability for medical care providers and patients. The participating centers for rare diseases see benefits in the structured registration of the patients and the simplification of cross-institutional patient management, as patients can fulfil more tasks on their own and the health professionals can easily share data. The development of the Telematikinfrastructure of the Gematik offers opportunities to ease the digital connection between doctors' offices and the center for rare diseases. In particular, constant clarification and transparency are essential in order to provide information on data protection issues. Training and support should also be provided to promote patients' digital skills.
Effects of percutaneous coronary intervention on dyspnea in stable coronary artery disease
Creators: name: Wester, Michael
name: Koll, Franziska
name: Luedde, Mark
name: Langer, Christoph
name: Resch, Markus
name: Luchner, Andreas
name: Müller, Karolina
name: Zeman, Florian
name: Koller, Michael
name: Maier, Lars S.
name: Sossalla, Samuel
Publisher: name: Universität Regensburg
Year: 2022
Subjects: subject: PCI, Stable CAD, QoL, PLA-pCi-EBO, Dyspnea
subject: 610 Medizin

description: Background
Dyspnea is a frequent symptom in patients with stable coronary artery disease (CAD) and is recognized as a possible angina equivalent.
Objectives
This study was to assess the impact of percutaneous coronary intervention (PCI) on dyspnea, quality of life, and angina pectoris in patients with stable CAD.
Methods
The prospective, multi-center PLA-pCi-EBO-pilot trial included 144 patients with symptomatic stable CAD and successful PCI. The prespecified endpoints angina pectoris (Seattle Angina Questionnaire–SAQ) and dyspnea (NYHA scale) were assessed 6 months after PCI. Predictors for symptomatic improvement were assessed with uni- and multivariable logistic regression analyses.
Results
Patients with concomitant dyspnea had worse SAQ physical limitation scores at baseline (49.5 ± 21.0 vs 58.9 ± 22.0, p = 0.013) but showed no difference for angina frequency or quality of life. Overall, symptomatic burden of angina pectoris and dyspnea was alleviated by PCI. However, patients with concomitant dyspnea had markedly worse scores for physical limitation (78.9 ± 25.0 vs 94.3 ± 10.6, p < 0.001), angina frequency (77.9 ± 22.8 vs 91.1 ± 12.4, p < 0.001), and quality of life (69.4 ± 24.1 vs 82.5 ± 14.4, p < 0.001) after PCI. The prevalence of dyspnea (NYHA class ≥ 2) declined from 73% before PCI to 54%. Of 95 initially dyspneic patients, 57 (60%) improved at least one NYHA class 6 months after PCI. In a multivariable logistic regression analysis, “atypical angina pectoris” was associated with improved NYHA class, whereas “diabetes mellitus” had a negative association.
Conclusion
PCI effectively reduced dyspnea, which is a frequent and demanding symptom in patients with CAD. The German Clinical Trials Register registration number is DRKS0001752 (www.drks.de).
Additional file 2: of Linking rare and common disease: mapping clinical disease-phenotypes to ontologies in therapeutic target validation
Creators: name: Sirarat Sarntivijai
name: Drashtti Vasant
name: Jupp, Simon
name: Saunders, Gary
name: A. Bento
name: Gonzalez, Daniel
name: Betts, Joanna
name: Samiul Hasan
name: Koscielny, Gautier
name: Dunham, Ian
name: Parkinson, Helen
name: Malone, James
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biochemistry
subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Pharmacology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: Marine Biology
subject: Cancer
subject: Science Policy

description: The list of journals mined for disease-phenotype assocations. (PDF 13 kb)
Additional file 1: of Effects of Greenselect PhytosomeÂŽ on weight maintenance after weight loss in obese women: a randomized placebo-controlled study
Creators: name: Gilardini, Luisa
name: Pasqualinotto, Lucia
name: Pierro, Francesco Di
name: Risso, Paolo
name: Invitti, Cecilia
Publisher: name: Figshare
Year: 2016
Subjects: subject: Medicine
subject: Physiology
subject: FOS: Biological sciences
subject: Sociology
subject: FOS: Sociology
subject: Developmental Biology
subject: Computational Biology

description: Questionnaire for the evaluation of adherence to the diet. (DOCX 16Â kb)
Cynicostola Meyrick 1925
Creators: name: Das, Apurva
name: Pathania, Prakash C.
name: Singh, Navneet
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Lepidoptera
Lecithoceridae
Cynicostola

description: Genus Cynicostola Meyrick, 1925: 230
Type species: Onebala pogonias Meyrick, 1923 c, by original designation
description: Published as part of Das, Apurva, Pathania, Prakash C. & Singh, Navneet, 2022, A catalogue of Indian Lecithoceridae (Lepidoptera: Gelechioidea), pp. 56-78 in Zootaxa 5165 (1) on page 71, DOI: 10.11646/zootaxa.5165.1.2, http://zenodo.org/record/6825526
Clevelandella kidderi Mandal and Nair 1974
Creators: name: Kotyk, Michael
name: Bourland, William A.
name: Soviš, Matyáš
name: Méndez-Sánchez, Daniel
name: Škaloud, Pavel
name: Varadínová, Zuzana Kotyková
name: Čepička, Ivan
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Chromista
Ciliophora
Heterotrichea
Heterotrichida
Clevelandellidae
Clevelandella
Clevelandella kidderi

description: Clevelandella kidderi Mandal and Nair, 1974
(Figs 30, 31; Supporting Information, Tables S41, S42)
One population (ASS) was morphologically characterized by both in vivo observations and in protargol preparations and one (PA) only in vivo. The ciliate was moderately to highly abundant.
Description based on ASS and PA populations: Medium-sized Clevelandella (on average 128 × 58 µm in vivo, range 101–168 × 44–72 µm; on average 94 × 50 µm in protargol preparations, range 84–107 × 42–59 µm). Spade-shaped, peristomial projection about 32% of body length, conspicuous left-sided notch at junction of peristomial projection and body proper, left lobe well developed. Macronucleus inverted teardrop-shape with finely granular chromatin, obliquely oriented, situated right of midline in anterior half of body proper. Karyophore at posterior end of macronucleus, visible inconsistently (Fig. 30E). Micronucleus globular near anterior margin of macronucleus. Somatic cilia limited to approximately anterior 60% of cell. Right sutural kinetofragments probably developed (Fig. 30C), but better oriented and stained cells would be needed to know for sure. Adoral zone extends about 58% of cell length on average, composed of an average of 47 membranelles (range 45–49). POM as described for the family (Fig. 30D).
description: Published as part of Kotyk, Michael, Bourland, William A., Soviš, Matyáš, Méndez-Sánchez, Daniel, Škaloud, Pavel, Varadínová, Zuzana Kotyková & Čepička, Ivan, 2024, Morphology maưers: congruence of morphology and phylogeny in the integrative taxonomy of Clevelandellidae (Ciliophora: Armophorea) with description of six new species, pp. 1-51 in Zoological Journal of the Linnean Society 202 (1) on page 30, DOI: 10.1093/zoolinnean/zlad154, http://zenodo.org/record/14541981
Additional file 1: Figure S1. of Characterization of HAF-4- and HAF-9-localizing organelles as distinct organelles in Caenorhabditis elegans intestinal cells
Creators: name: Tanji, Takahiro
name: Nishikori, Kenji
name: Syoko Haga
name: Kanno, Yuki
name: Kobayashi, Yusuke
name: Takaya, Mai
name: Gengyo-Ando, Keiko
name: Mitani, Shohei
name: Shiraishi, Hirohisa
name: Ohashi-Kobayashi, Ayako
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biophysics
subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Molecular Biology
subject: Physiology
subject: FOS: Biological sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Staining of Is[haf-4::GFP] (a) and Is[haf-9::GFP] (b) with the lysochrome dye Nile Red after fixation. Figure S2. DHS-3-positive intestinal granules are not autofluorescent granules. Figure S3. lmp-1 is not required for the biogenesis of DHS-3::GFP-positive lipid droplets. Figure S4. VIT-2::GFP did not localize to either the autofluorescent granules or the lipid droplets. Figure S5. LMP-1::mRFP did not localize to VIT-2::GFP-positive granules. Figure S6. GLO-1::GFP-positive granules are autofluorescent but not Nile Red-positive. Figure S7. LMP-1::mRFP did not localize to GLO-1::GFP-positive granules. Figure S8. glo-1 is not required for the biogenesis of DHS-3::GFP-positive lipid droplets. Figure S9. HAF-4 and HAF-9 did not localize to either the peroxisomes or the mitochondria. Figure S10. GFP::RAB-7 did not localize to either the autofluorescent granules or the Nile Red-positive lipid droplets. Figure S11. rab-7 is required for the biogenesis of LMP-1::mCherry-positive granules. Figure S12. rab-7 is not required for the biogenesis of autofluorescent granules and DHS-3::GFP-positive lipid droplets. Figure S13. Other representative images. (PDF 5985 kb)
MOESM4 of Emerging knock-down resistance in Anopheles arabiensis populations of Dakar, Senegal: first evidence of a high prevalence of kdr-e mutation in West African urban area
Creators: name: Ndiath, Mamadou
name: Cailleau, Aurélie
name: Orlandi-Pradines, Eve
name: Bessell, Paul
name: Fréderic Pagès
name: Jean-François Trape
name: Rogier, Christophe
Publisher: name: Figshare
Year: 2015
Subjects: subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Genetics
subject: Biotechnology
subject: Evolutionary Biology
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Additional file 4. Insecticide-induced mortality of mosquitoes in Pikine, Yarakh and Almadies with and without prior exposure to PBO.
Paraclevelandia brevis Kidder 1937
Creators: name: Kotyk, Michael
name: Bourland, William A.
name: Soviš, Matyáš
name: Méndez-Sánchez, Daniel
name: Škaloud, Pavel
name: Varadínová, Zuzana Kotyková
name: Čepička, Ivan
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Chromista
Ciliophora
Clevelandellidae
Paraclevelandia
Paraclevelandia brevis

description: Paraclevelandia brevis in ‘brevis’ form
(Figs 23A–G, 24A, B, D, E;
Supporting Information, Tables S25–S29)
When present, the ‘brevis’ form was highly abundant.
Description based on ASS, PAA, PAC, PRG, STH in vivo, and PRG and STH in protargol preparation: Small Clevelandellidae (on average 44 × 28 µm in vivo, range 34–58 × 21–48 µm; on average 34 × 21 in protargol preparations, range 26–47 × 15–28 µm). Broadly ovate, slightly dorsoventrally flattened, anterior end tapers to rounded point, posterior end transversely truncate. Posterior peristomial projection absent. Peristomial overture on left posterior margin of cell. Macronucleus elongated ellipsoidal, chromatin coarsely granular. Micronucleus ellipsoidal, relatively large (4–6 µm across), dorsal to macronucleus (Fig. 23D). Karyophore not detected. A sack-like structure formed between anterior margin of macronucleus and anterior end of cell as reported by Kidder (1937, 1938), not evident in protargol preparations (Fig. 23A). Cortex with refractile interkinetal granules, probably mucocysts (Fig. 23B). Somatic kineties strongly spiralled leftward (Fig. 24B). Somatic cilia absent on right, ventral, left parts of posterior cortex (Fig. 24A, B, E), extend to posterior cell margin dorsally. Kinetal furrows prominent. Two ciliated circumperistomial kineties. About 10 ciliated kinetofragments in midportion of right suture, increase in length from posterior to anterior (Figs 23E, F, 24B, D). Organization of oral ciliature as for the family except, in absence of peristomial projection, oral structures are situated in body proper (Fig. 23A, B, D, H, J). Adoral zone narrows from posterior to anterior, extends about 50% of cell length on average, composed of an average of 15 membranelles (range 14–17).
description: Published as part of Kotyk, Michael, Bourland, William A., Soviš, Matyáš, Méndez-Sánchez, Daniel, Škaloud, Pavel, Varadínová, Zuzana Kotyková & Čepička, Ivan, 2024, Morphology maưers: congruence of morphology and phylogeny in the integrative taxonomy of Clevelandellidae (Ciliophora: Armophorea) with description of six new species, pp. 1-51 in Zoological Journal of the Linnean Society 202 (1) on page 22, DOI: 10.1093/zoolinnean/zlad154, http://zenodo.org/record/14541981
Additional file 1: of Hepatoprotective activity of Lepidium sativum seeds against D-galactosamine/lipopolysaccharide induced hepatotoxicity in animal model
Creators: name: Raish, Mohammad
name: Ahmad, Ajaz
name: Alkharfy, Khalid
name: Ahamad, Syed
name: Mohsin, Kazi
name: Al-Jenoobi, Fahad
name: Al-Mohizea, Abdullah
name: Ansari, Mushtaq
Publisher: name: Figshare
Year: 2016
Subjects: subject: Molecular Biology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: Developmental Biology
subject: Marine Biology
subject: Cancer

description: Supplementary data file 1. (DOCX 64Â kb)
Nematopus indus
Creators: name: Brailovsky, Harry
Publisher: name: Zenodo
Year: 2015
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hemiptera
Coreidae
Nematopus
Nematopus indus

description: Nematopus indus (Linnaeus, 1758)
(Fig. 7)
Cimex indus Linnaeus, 1758: 447.
Material examined. FRENCH GUYANA: 3 ♁♁, Remire, 29.iii.1986, P. J. Clausen (UMSP); 1 ♀, Barrage de Petit Saut, 15.i.1998, P. Cerdan (MNHN); 1 ♁, Maison Vidal, 4.ix.1992, P. Gombauld (MNHN); 1♀, Demerara, Timebri, 0–50 m, 6°30 ′ N 58°15 ′ W, 24.i.1981, J. Longino (LACM); 1 ♁, 14 km E of N2 on Road to Degrad Correze, 108 m a.s.l., 04°29 ′ N 050°20 ′ W, 6.xii.2002, J. E. Eger (FSCA); 25 ♁♁ 16 ♀♀, Mana River, iii.1917, v.1917, vi.1917 (CMNH); 1 ♀, Roroia, x.1977 (MNHN); 1 ♁, Camopi, 1900, F. Geay (MNHN); 2 ♁♁, Les Roches de Kovrov, 1907, E. Le Moult (MNHN); 1 ♀, Kourou, km 15 SW, 6.viii.1976, D. Roubik (UNAM); 2 ♁♁, Reserve Naturelle de la Trivite, Roche Benitier, Comune de Mana, 4 °37 ′ 9.3 ″ N 53°24 ′ 32.3 ″ W, 8.xii.2010 (Piege vitre), S. Brulle, P. H. Dalens and S. Fernandez (MNHN); 11 ♁♁ 7 ♀♀, Point de vue du Belvedere de Saül, 250 m a.s.l., window trap on the top of the mountain, 3°37 ′ 22 ″ N 53°12 ′ 34 ″ W, 28.ii.2010, 30.xi.2010, 11.i.2011, 23.ii.2011, 14.–30.iii.2011, 20.–23.v.2011, 15.vi.2011, 15.vii.2011, 20.–24.xii.2011, S. Brulle, P.H. Dalens, and S. Fernandez (MNHN, UNAM); 5 ♁♁ 2 ♀♀, Montagne des Cheveaux, 20 m a.s.l., window trap, 04°43 ′ 0.12 ″ N 52°25 ′ 0.12 ″ W, 10.x.2007, 11.–23. xii.2011, 17.ii.2012, 2.xii.2012, 13.i.2013, S. Brulle, P. H. Dalens and S. Fernandez (MNHN, UNAM); 3 ♁♁ 7 ♀♀, Amazon Nature Lodge, 30 km SE Roura on Kaw Road, 300 m a.s.l., 04°33 ′ 57 ″ N 05°12 ′ 43 ″ W, 2.–13.vi.2005, 10.–18.iv.2007, J. E. Eger, D. G. Hall, and M. T. Messenger (FSCA, UNAM); 1 ♁ 2 ♀♀, vic. Remire, E of Cayenne, 21.iv.2007, J. E. Eger (FSCA, UNAM).
Diagnosis. This species is recognized by having the antennal segment IV black with yellow ring near basal third; antennal segments II and III black with basal joint yellow; connexivum and abdominal sterna III to VII shiny orange; male genital capsule and female genital plates black; thorax including the anterior and posterior lobe of metathoracic peritreme black (eventually the anterior lobe is dark yellow); femora entirely black; fore and middle tibiae black with apical third dark yellow; hind tibiae dark yellow with basal third black.
Distribution. Argentina, Bolivia, Brazil, Chile, French Guiana, Guyana, Suriname, Venezuela (AMYOT & SERVILLE 1843, WALKER 1871, PACKAUSKAS 2010). In French Guiana cited only from Cayenne (WALKER 1871).
description: Published as part of Brailovsky, Harry, 2015, The genus Nematopus (Hemiptera: Heteroptera: Coreidae) of French Guiana with a description of one new species, pp. 39-45 in Acta Entomologica Musei Nationalis Pragae (suppl.) (suppl.) 55 (1) on page 44, DOI: 10.5281/zenodo.4468281
Cynicostola pogonias Onebala
Creators: name: Das, Apurva
name: Pathania, Prakash C.
name: Singh, Navneet
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Lepidoptera
Lecithoceridae
Cynicostola
Cynicostola pogonias

description: 118. Cynicostola pogonias (Meyrick, 1923 c: 43) (Onebala)
Type locality: India, Tamil Nadu, Nilgiris
Distribution: Indian records: Tamil Nadu.
description: Published as part of Das, Apurva, Pathania, Prakash C. & Singh, Navneet, 2022, A catalogue of Indian Lecithoceridae (Lepidoptera: Gelechioidea), pp. 56-78 in Zootaxa 5165 (1) on page 71, DOI: 10.11646/zootaxa.5165.1.2, http://zenodo.org/record/6825526
Rheo-SINDy: Finding a Constitutive Model from Rheological Data for Complex Fluids Using Sparse Identification for Nonlinear Dynamics
Creators: name: Sato, Takeshi
name: Miyamoto, Souta
name: Kato, Shota
Publisher: name: arXiv
Year: 2024
Subjects: subject: Soft Condensed Matter (cond-mat.soft)
subject: FOS: Physical sciences

description: Rheology plays a pivotal role in understanding the flow behavior of fluids by discovering governing equations that relate deformation and stress, known as constitutive equations. Despite the importance of these equations, current methods for deriving them lack a systematic methodology, often relying on sense of physics and incurring substantial costs. To overcome this problem, we propose a novel method named Rheo-SINDy, which employs the sparse identification of nonlinear dynamics (SINDy) algorithm for discovering constitutive models from rheological data. Rheo-SINDy was applied to five distinct scenarios, four with well-established constitutive equations and one without predefined equations. Our results demonstrate that Rheo-SINDy successfully identified accurate models for the known constitutive equations and derived physically plausible approximate models for the scenario without established equations. Notably, the identified approximate models can accurately reproduce nonlinear shear rheological properties, especially at steady state, including shear thinning. These findings validate the robustness of Rheo-SINDy in handling data complexities and underscore its efficacy as a tool for advancing the development of data-driven approaches in rheology.
description: 19 pages, 17 figures
Additional file 2: Figure S2. of Identification and functional analysis of two Golgi-localized UDP-galactofuranose transporters with overlapping functions in Aspergillus niger
Creators: name: Joohae Park
name: Tefsen, Boris
name: Heemskerk, Marc
name: Lagendijk, Ellen
name: Hondel, Cees Van Den
name: Die, Irma Van
name: Ram, Arthur
Publisher: name: Figshare
Year: 2015
Subjects: subject: Biophysics
subject: Biochemistry
subject: 29999 Physical Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: Microbiology
subject: FOS: Biological sciences
subject: Genetics
subject: Molecular Biology
subject: Physiology
subject: Pharmacology
subject: Biotechnology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: 60506 Virology

description: Amino acid alignment of A. niger UgtA and UgtB proteins with UgtA/GlfB of A. fumigatus. Transmembrane domains predicted in UgtA/GlfB and homologous region in the A. niger proteins are highlighted. (DOCX 16 kb)