title: Ð ÐµÐ³ÑÐ»ÑÑÐ¸Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ð¾Ð² 1 Ð¸ 4 ÑÐ¸Ð¿Ð° Ð¿ÑÐ¸ ÑÐ¾ÑÑÐ´Ð¸ÑÑÐ¾Ð¼ ÑÐ¸Ð±ÑÐ¾Ð·Ðµ Ñ ÐºÑÑÑ Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ Ð¼Ð¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ñ ÑÐ¾Ð½Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð¿Ð¾ÑÐµÑÐ½Ð¾Ð¹ Ð½ÐµÐ´Ð¾ÑÑÐ°ÑÐ¾ÑÐ½Ð¾ÑÑÐ¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: The regulation of collagen type 1 and 4 expression in vascular fibrosis in rats in a model of chronic renal failure
Creators: name: ÐÑÑÐ½ÐºÐ¾Ð², ÐÑÑÐµÐ½Ð¸Ð¹
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: ÑÐ¾ÑÑÐ´Ð¸ÑÑÑÐ¹ ÑÐ¸Ð±ÑÐ¾Ð·
Ð¥ÐÐ
Ð½ÐµÑÑÑÐºÑÐ¾Ð¼Ð¸Ñ
fli1
ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½ I
ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½ IV
vascular fibrosis
ckd
nephrectomy
collagen I
collagen IV

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ ÑÐ¸Ð±ÑÐ¾Ð·Ð° Ð¾ÑÐ³Ð°Ð½Ð¾Ð² ÑÐµÑÐ´ÐµÑÐ½Ð¾-ÑÐ¾ÑÑÐ´Ð¸ÑÑÐ¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ñ Ñ ÐºÑÑÑ Ð¿ÑÐ¸ Ð¼Ð¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ ÑÑÐ¾Ð½Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð¿Ð¾ÑÐµÑÐ½Ð¾Ð¹ Ð½Ðµ-Ð´Ð¾ÑÑÐ°ÑÐ¾ÑÐ½Ð¾ÑÑÐ¸ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð½ÐµÑÑÐµÐºÑÐ¾Ð¼Ð¸Ð¸. Ð¦ÐµÐ»Ñ ÑÐ°Ð±Ð¾ÑÑ: Ð¾ÑÐµÐ½ÐºÐ° ÑÑÐµÐ¿ÐµÐ½Ð¸ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ñ ÑÐ¸Ð±ÑÐ¾Ð·Ð° Ð² Ð±ÑÑÑÐ½Ð¾Ð¹ Ð°Ð¾ÑÑÐµ Ð¸ ÑÐµÑÐ´ÑÐµ Ñ ÐºÑÑÑ Ð»Ð¸Ð½Ð¸Ð¸ Sprague-Dawley Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ Ð¼Ð¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¥ÐÐ. Ð ÑÐ¾Ð´Ðµ ÑÐ°Ð±Ð¾ÑÑ ÑÐµÑÐ°Ð»Ð¸ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð·Ð°Ð´Ð°ÑÐ¸: 1. ÐÑÐµÐ½Ð¸ÑÑ ÑÑÐ¾Ð²Ð½Ð¸ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð², ÐºÐ¾Ð´Ð¸ÑÑÑÑÐ¸Ñ ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ñ I Ð¸ IV, Ð° ÑÐ°ÐºÐ¶Ðµ ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸Ð¾Ð½Ð½Ð¾Ð³Ð¾ ÑÐ°ÐºÑÐ¾ÑÐ° Fli1 Ð² Ð±ÑÑÑÐ½Ð¾Ð¹ ÑÐ°ÑÑÐ¸ Ð°Ð¾ÑÑÑ Ð¸ Ð² ÑÐµÑÐ´ÑÐµ; 2. ÐÑÐ¾Ð²ÐµÑÑÐ¸ ÐºÐ¾ÑÑÐµÐ»ÑÑÐ¸Ð¾Ð½Ð½ÑÐ¹ Ð°Ð½Ð°-Ð»Ð¸Ð· ÑÑÐ¾Ð²Ð½ÐµÐ¹ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð², ÐºÐ¾Ð´Ð¸ÑÑÑÑÐ¸Ñ ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½ I Ð¸ ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸Ð¾Ð½-Ð½ÑÐ¹ ÑÐ°ÐºÑÐ¾Ñ Fli1; 3. ÐÑÐµÐ½Ð¸ÑÑ ÑÑÐ¾Ð²ÐµÐ½Ñ Ð±ÐµÐ»ÐºÐ¾Ð² Fli1 Ð¸ ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ð° I Ð² Ð±ÑÑÑÐ½Ð¾Ð¹ ÑÐ°ÑÑÐ¸ Ð°Ð¾ÑÑÑ; 4. Ð¡ÑÐ°Ð²Ð½Ð¸ÑÑ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÐµ Ð½Ð° Ð´Ð²ÑÑ Ð»Ð¸Ð½Ð¸ÑÑ ÐºÑÑÑ (Sprague-Dawley Ð¸ Wistar); 5. Ð¡ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð³Ð¸ÑÑÐ¾ÑÐ¸Ð¼Ð¸Ð¸ Ð¸ Ð¸Ð¼Ð¼ÑÐ½Ð¾-Ð³Ð¸ÑÑÐ¾ÑÐ¸Ð¼Ð¸Ð¸ Ð¿ÑÐ¾Ð²ÐµÑÑÐ¸ Ð°Ð½Ð°Ð»Ð¸Ð· ÑÑÐ¾Ð²Ð½Ñ ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ð¾Ð² Ð² Ð±ÑÑÑÐ½Ð¾Ð¹ Ð°Ð¾ÑÑÐµ Ð¸ ÑÐ¾ÑÑ-Ð´Ð°Ñ Ð¼Ð¾Ð·Ð³Ð° ÐºÑÑÑ Ð»Ð¸Ð½Ð¸Ð¸ Sprague-Dawley. Ð Ð°Ð±Ð¾ÑÐ° Ð±ÑÐ»Ð° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð² Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ Ð¸Ð½ÑÐµÐ³ÑÐ°ÑÐ¸Ð²Ð½Ð¾Ð¹ Ð½ÐµÐ¹ÑÐ¾ÑÐ½Ð´Ð¾-ÐºÑÐ¸Ð½Ð¾Ð»Ð¾Ð³Ð¸Ð¸ Ð² ÐÐ­Ð¤Ð Ð¸Ð¼. Ð.Ð. Ð¡ÐµÑÐµÐ½Ð¾Ð²Ð°. Ð ÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð¼ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÐµÑÑÑ Ð¿Ð¾Ð´ÑÐ²ÐµÑÐ¶Ð´ÐµÐ½Ð¸Ðµ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ñ ÑÐ¸Ð±ÑÐ¾Ð·Ð° Ð² ÑÐ¾-ÑÑÐ´Ð¸ÑÑÐ¾Ð¹ ÑÑÐµÐ½ÐºÐµ Ð¸ Ð»ÐµÐ²Ð¾Ð¼ Ð¶ÐµÐ»ÑÐ´Ð¾ÑÐºÐµ ÐºÑÑÑ Ð»Ð¸Ð½Ð¸Ð¸ Sprague-Dawley (Ð¿Ð¾Ð²ÑÑÐµ-Ð½Ð¸Ðµ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Col1A1 Ð¸ Col4A1, Ð½Ð°ÐºÐ¾Ð¿Ð»ÐµÐ½Ð¸Ðµ ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ð¾Ð² Ð² ÑÐºÐ°Ð½Ð¸). Ð£ ÐºÑÑÑ Wistar ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ðµ ÑÐ¸Ð±ÑÐ¾Ð·Ð° Ð½Ðµ Ð½Ð°Ð±Ð»ÑÐ´Ð°ÐµÑÑÑ, ÑÑÐ¾ Ð¼Ð¾Ð¶Ð½Ð¾ Ð¾Ð±ÑÑÑÐ½Ð¸ÑÑ Ð°ÐºÑÐ¸-Ð²Ð°ÑÐ¸ÐµÐ¹ Ð¸Ð½ÑÑ ÐºÐ¾Ð¼Ð¿ÐµÐ½ÑÐ°ÑÐ¾ÑÐ½ÑÑ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð¾Ð². ÐÐ¾Ð´ÐµÐ»Ñ Ð¥ÐÐ Ð½Ð° ÐºÑÑÑÐ°Ñ Ð»Ð¸Ð½Ð¸Ð¸ Sprague-Dawley Ð¼Ð¾Ð¶ÐµÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°ÑÑÑÑ Ð´Ð»Ñ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ¸ Ð¿ÑÑÐµÐ¹ ÑÐ°ÑÐ¼Ð°ÐºÐ¾Ð»Ð¾-Ð³Ð¸ÑÐµÑÐºÐ¾Ð¹ ÐºÐ¾ÑÑÐµÐºÑÐ¸Ð¸ ÑÐ¸Ð±ÑÐ¾Ð·Ð°.
description: This work is devoted to the study of cardiovascular fibrosis in rats under chronic renal failure modeling by nephrectomy. Objective: to assess the degree of fibrosis development in the abdominal aorta and heart in Sprague-Dawley rats under conditions of chronic renal fail-ure modeling. The following task were assigned: 1. Estimate the expression levels of genes encoding collagens I and IV, transcription factor Fli1 in ab-dominal aorta and heart; 2. Perform correlation analysis of expression levels of genes encoding collagen I and transcription factor Fli1; 3. Estimate the levels of protein Fli1 and collagen I in abdominal part of aorta; 4. Compare the results obtained in two rat lines (Sprague-Dawley and Wistar); 5. Analyze collagen level in abdominal aorta and brain vessels of Sprague-Dawley line rats using histochemistry and immunohistochemistry methods. The work was performed at the laboratory of integrative neuroendocri-nology at Sechenov IEFB. The work resulted in confirmation of fibrosis development in the vascu-lar wall and left ventricle of Sprague-Dawley rats (increased expression of Col1A1 and Col4A1 genes, collagen accumulation in the tissue). The develop-ment of fibrosis was not observed in Wistar rats, which can be explained by activation of other compensatory mechanisms. The CRF model in Sprague-Dawley rats can be used to develop ways of pharmacological correction of fi-brosis.