title: ÐÐ½Ð°Ð»Ð¸Ð· Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¸ ÑÑÐ½ÐºÑÐ¸Ð¹ Ð³Ð»Ð¸Ð°Ð»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð½Ð° Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ ÐÐ»ÑÑÐ³ÐµÐ¹Ð¼ÐµÑÐ° Drosophila melanogaster
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Analysis of the morphology and functions of glial cells in the model of Alzheimer's disease Drosophila melanogaster
Creators: name: Ð¡ÑÑÐ¸Ð½Ð°, ÐÐ¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: Ð±Ð¾Ð»ÐµÐ·Ð½Ñ ÐÐ»ÑÑÐ³ÐµÐ¹Ð¼ÐµÑÐ°
Ð±ÐµÐ»Ð¾Ðº Ð¿ÑÐµÐ´ÑÐµÑÑÐ²ÐµÐ½Ð½Ð¸Ðº Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð°
drosophila melanogaster
Ð³Ð»Ð¸Ð°Ð»ÑÐ½ÑÐµ ÐºÐ»ÐµÑÐºÐ¸
Ð³ÐµÐ¼Ð°ÑÐ¾ÑÐ½ÑÐµÑÐ°Ð»Ð¸ÑÐµÑÐºÐ¸Ð¹ Ð±Ð°ÑÑÐµÑ
Alzheimer's disease
amyloid precursor protein
glial cells
blood-brain barrier

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð°Ð½Ð°Ð»Ð¸Ð·Ñ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð³Ð»Ð¸Ð°Ð»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº, Ð² ÑÐ°ÑÑÐ½Ð¾ÑÑÐ¸, ÐºÐ»ÐµÑÐ¾Ðº Ð¿Ð¾Ð²ÐµÑÑÐ½Ð¾ÑÑÐ½Ð¾Ð¹ Ð³Ð»Ð¸Ð¸ Ð¸ Ð³Ð»Ð¸Ð¸ ÐºÐ¾ÑÑÐµÐºÑÐ°, Ð½Ð° Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ ÐÐ»ÑÑÐ³ÐµÐ¹Ð¼ÐµÑÐ° Ñ Drosophila melanogaster, Ð° ÑÐ°ÐºÐ¶Ðµ Ð°Ð½Ð°Ð»Ð¸Ð·Ñ ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð³ÐµÐ¼Ð°ÑÐ¾ÑÐ½ÑÐµÑÐ°Ð»Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð±Ð°ÑÑÐµÑÐ° Ð¿ÑÐ¸ Ð¼Ð¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ ÐÐ»ÑÑÐ³ÐµÐ¹Ð¼ÐµÑÐ°. ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ Ð±ÑÐ»Ð¸ Ð¿Ð¾ÑÑÐ°Ð²Ð»ÐµÐ½Ñ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: 1. ÐÑÐµÐ½ÐºÐ° Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¿ÐµÑÐ¸Ð½ÐµÐ²ÑÐ°Ð»ÑÐ½Ð¾Ð¹, ÑÑÐ±Ð¿ÐµÑÐ¸Ð½ÐµÐ²ÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð³Ð»Ð¸Ð¸ Ð¸ Ð³Ð»Ð¸Ð¸ ÐºÐ¾ÑÑÐµÐºÑÐ° Ð² ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½ÑÑ Ð»Ð¸Ð½Ð¸ÑÑ Ð¸ Ð² ÑÑÐ°Ð½ÑÐ³ÐµÐ½Ð½ÑÑ Ð»Ð¸Ð½Ð¸ÑÑ Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸ÐµÐ¹ APP Ð¸ BACE ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°. 2. ÐÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð° ÑÐ´ÐµÑ ÐºÐ»ÐµÑÐ¾Ðº Ð¿ÐµÑÐ¸Ð½ÐµÐ²ÑÐ°Ð»ÑÐ½Ð¾Ð¹, ÑÑÐ±Ð¿ÐµÑÐ¸Ð½ÐµÐ²ÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð³Ð»Ð¸Ð¸ Ð¸ Ð³Ð»Ð¸Ð¸ ÐºÐ¾ÑÑÐµÐºÑÐ° Ð² ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½ÑÑ Ð»Ð¸Ð½Ð¸ÑÑ Ð¸ Ð² ÑÑÐ°Ð½ÑÐ³ÐµÐ½Ð½ÑÑ Ð»Ð¸Ð½Ð¸ÑÑ Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸ÐµÐ¹ APP Ð¸ BACE ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°. 3. ÐÑÐµÐ½ÐºÐ° ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð°Ð»ÑÐ½Ð¾Ð¹ ÑÐµÐ»Ð¾ÑÑÐ½Ð¾ÑÑÐ¸ Ð³ÐµÐ¼Ð°ÑÐ¾ÑÐ½ÑÐµÑÐ°Ð»Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð±Ð°ÑÑÐµÑÐ° Ð² ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½Ð¾Ð¹ Ð»Ð¸Ð½Ð¸Ð¸ Ð¸ Ð² ÑÑÐ°Ð½ÑÐ³ÐµÐ½Ð½Ð¾Ð¹ Ð»Ð¸Ð½Ð¸Ð¸ Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸ÐµÐ¹ APP Ð¸ BACE ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð² ÐºÐ»ÐµÑÐºÐ°Ñ ÑÑÐ±Ð¿ÐµÑÐ¸Ð½ÐµÐ²ÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð³Ð»Ð¸Ð¸. Ð Ð°Ð±Ð¾ÑÐ° Ð±ÑÐ»Ð° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ Ð¤ÐµÐ´ÐµÑÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð³Ð¾ÑÑÐ´Ð°ÑÑÑÐ²ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð±ÑÐ´Ð¶ÐµÑÐ½Ð¾Ð³Ð¾ ÑÑÑÐµÐ¶Ð´ÐµÐ½Ð¸Ñ Â«ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¸Ð½ÑÑÐ¸ÑÑÑ ÑÐ´ÐµÑÐ½Ð¾Ð¹ ÑÐ¸Ð·Ð¸ÐºÐ¸ Ð¸Ð¼. Ð.Ð. ÐÐ¾Ð½ÑÑÐ°Ð½ÑÐ¸Ð½Ð¾Ð²Ð° ÐÐ°ÑÐ¸Ð¾Ð½Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÑÐºÐ¾Ð³Ð¾ ÑÐµÐ½ÑÑÐ° Â«ÐÑÑÑÐ°ÑÐ¾Ð²ÑÐºÐ¸Ð¹ Ð¸Ð½ÑÑÐ¸ÑÑÑÂ» Ð² Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð¸ Ð¿ÑÐ¸ÐºÐ»Ð°Ð´Ð½Ð¾Ð¹ Ð³ÐµÐ½ÐµÑÐ¸ÐºÐ¸ Ð¿Ð¾Ð´ ÑÑÐºÐ¾Ð²Ð¾Ð´ÑÑÐ²Ð¾Ð¼ Ð´Ð¾ÐºÑÐ¾ÑÐ° Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð½Ð°ÑÐº Ð¡Ð²ÐµÑÐ»Ð°Ð½Ñ ÐÐ»Ð°Ð´Ð¸Ð¼Ð¸ÑÐ¾Ð²Ð½Ñ Ð¡Ð°ÑÐ°Ð½ÑÐµÐ²Ð¾Ð¹.
description: This work is devoted to the analysis of the morphology of glial cells, in particular surface glia and cortex glia cells, on the model of Alzheimer's disease in Drosophila melanogaster, as well as the analysis of the functioning of the blood-brain barrier in modeling Alzheimer's disease. The tasks that were set during the study: 1. Evaluation of the morphology of perineural, subperineural glia and cortex glia in control lines and transgenic lines with expression of human APP and BACE. 2. Determination of the number of cell nuclei of perineural, subperineural glia and cortex glia in control lines and in transgenic lines with expression of human APP and BACE. 3. Assessment of the functional integrity of the blood-brain barrier in the control line and in the transgenic line with the expression of human APP and BACE in subperineural glia cells. The work was carried out on the basis of the Federal State Budgetary Institution Petersburg Institute of Nuclear Physics named after B.P. Konstantinov National Research Center Â«Kurchatov InstituteÂ» in the laboratory of experimental and applied genetics under the guidance of Doctor of Biological Sciences Svetlana Vladimirovna Sarantseva.