title: ÐÑÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð³ÐµÐ½Ð¾Ð¼Ð½Ð¾Ð¹ ÐÐÐ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð¸Ð· DryBloodSpotÐ´Ð»Ñ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐ¸ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ð¹ Ð½Ð¾Ð²Ð¾ÑÐ¾Ð¶Ð´ÐµÐ½Ð½ÑÑ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Isolation of human genomic DNA from DryBloodSpot for the diagnosis of genetic diseases in newborns
Creators: name: ÐÐµÑÐµÐ·ÐµÐ½ÐºÐ¾Ð²Ð°, ÐÐ°ÑÐ°Ð»ÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: ÑÑÑ Ð¸Ðµ Ð¿ÑÑÐ½Ð° ÐºÑÐ¾Ð²Ð¸
Ð¼Ð°Ð³Ð½Ð¸ÑÐ½ÑÐµ ÑÐ°ÑÑÐ¸ÑÑ
Ð½ÐµÐ¾Ð½Ð°ÑÐ°Ð»ÑÐ½ÑÐ¹ ÑÐºÑÐ¸Ð½Ð¸Ð½Ð³
Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐ°
dry blood spots
magnetic beads
neonatal screening
diagnosis

description: Ð¦ÐµÐ»Ñ ÑÐ°Ð±Ð¾ÑÑ â ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°ÑÑ Ð½Ð°Ð±Ð¾Ñ Ð´Ð»Ñ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¸Ð· ÑÑÑÐ¸Ñ Ð¿ÑÑÐµÐ½ ÐºÑÐ¾Ð²Ð¸ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ ÑÐ¾ÑÐ±ÑÐ¸Ð¸ Ð½Ð° Ð¼Ð°Ð³Ð½Ð¸ÑÐ½ÑÑ ÑÐ°ÑÑÐ¸ÑÐ°Ñ ÐÐ°Ð³Ð½Ð¾Ð¡Ð-ÐÐ-ÐÐ¸Ð¾ (Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´Ð¸ÑÐµÐ»Ñ: ÐÐ ÐÐ»ÐºÐ¾Ñ ÐÐ¸Ð¾, Ð Ð¾ÑÑÐ¸Ñ). ÐÐ°Ð´Ð°ÑÐ¸: 1. ÐÐ·ÑÑÐµÐ½Ð¸Ðµ Ð½Ð°ÑÑÐ½Ð¾Ð¹ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÑ Ð¿Ð¾ ÑÐµÐ¼Ðµ ÑÐ°Ð±Ð¾ÑÑ 2. ÐÐ¾Ð´Ð±Ð¾Ñ Ð¾Ð¿ÑÐ¸Ð¼Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÐ¾ÑÑÐ°Ð²Ð° Ð½Ð°Ð±Ð¾ÑÐ° Ð´Ð»Ñ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¸Ð· ÑÑÑÐ¸Ñ Ð¿ÑÑÐµÐ½ ÐºÑÐ¾Ð²Ð¸ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ ÑÐ¾ÑÐ±ÑÐ¸Ð¸ Ð½Ð° Ð¼Ð°Ð³Ð½Ð¸ÑÐ½ÑÑ ÑÐ°ÑÑÐ¸ÑÐ°Ñ 3. Ð¡ÑÐ°Ð²Ð½ÐµÐ½Ð¸Ðµ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¸Ð· ÑÑÑÐ¸Ñ Ð¿ÑÑÐµÐ½ ÐºÑÐ¾Ð²Ð¸ Ð¾ÑÐ½Ð¾ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾ Ð²ÑÐ´ÐµÐ»Ð¸ÑÐµÐ»ÑÐ½ÑÑ Ð½Ð°Ð±Ð¾ÑÐ¾Ð² Ð´ÑÑÐ³Ð¸Ñ ÐºÐ¾Ð¼Ð¿Ð°Ð½Ð¸Ð¹ 4. ÐÐ¿ÑÐ¾Ð±Ð°ÑÐ¸Ñ Ð½Ð°Ð±Ð¾ÑÐ° Ð½Ð° Ð±Ð¾Ð»ÑÑÐµÐ¼ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ðµ Ð¾Ð±ÑÐ°Ð·ÑÐ¾Ð² 5. ÐÐ½Ð°Ð»Ð¸Ð· Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð² Ð ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð¾ÑÐ½Ð¾Ð²Ð½ÑÑ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð´Ð»Ñ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ¸ Ð½Ð°Ð±Ð¾ÑÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ Ð¼ÐµÑÐ¾Ð´ ÑÑÑÐ¸Ñ Ð¿ÑÑÐµÐ½, ÑÐºÑÑÑÐ°ÐºÑÐ¸Ñ Ð½ÑÐºÐ»ÐµÐ¸Ð½Ð¾Ð²ÑÑ ÐºÐ¸ÑÐ»Ð¾Ñ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ ÑÐ¾ÑÐ±ÑÐ¸Ð¸ Ð½Ð° Ð¼Ð°Ð³Ð½Ð¸ÑÐ½ÑÑ ÑÐ°ÑÑÐ¸ÑÐ°Ñ, Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð½Ð°Ñ ÑÐµÐ¿Ð½Ð°Ñ ÑÐµÐ°ÐºÑÐ¸Ñ (ÐÐ¦Ð ), Ð¸Ð·Ð¼ÐµÑÐµÐ½Ð¸Ðµ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¹ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ ÑÐ»ÑÐ¾ÑÐ¸Ð¼ÐµÑÑÐ¸Ð¸. ÐÑÐ¸ Ð½Ð°Ð¿Ð¸ÑÐ°Ð½Ð¸Ðµ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±Ð·Ð¾ÑÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ Scopus, PubMed, cyberleninka.ru, nature.com, Elibrary.ru Ð¡ÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÑÑ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»Ð¸ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿Ð°ÐºÐµÑÐ° Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼ Microsoft Excel ÐÐ¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð¾ÑÐºÐ»Ð¾Ð½ÐµÐ½Ð¸Ð¹ Ð½Ð° ÑÐ°Ð½Ð½Ð¸Ñ ÑÑÐ°Ð¿Ð°Ñ Ð¶Ð¸Ð·Ð½Ð¸ Ð½Ð¾Ð²Ð¾-ÑÐ¾Ð¶Ð´ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¿Ð¾Ð¼Ð¾Ð³Ð°ÐµÑ ÑÐ²Ð¾ÐµÐ²ÑÐµÐ¼ÐµÐ½Ð½Ð¾ Ð½Ð°Ð·Ð½Ð°ÑÐ¸ÑÑ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾Ðµ Ð»ÐµÑÐµÐ½Ð¸Ðµ Ð¸ Ð¿ÑÐµÐ´Ð¾ÑÐ²ÑÐ°ÑÐ¸ÑÑ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ðµ ÑÑÐ¶ÐµÐ»ÑÑ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÐ²Ð¸Ð¹. ÐÐµÑÐ¾Ð´ ÑÑÑÐ¸Ñ Ð¿ÑÑÐµÐ½ â ÑÑÐ¾ Ð¿ÑÐ¾ÑÑÐ¾Ð¹ Ð¸ ÑÐ´Ð¾Ð±Ð½ÑÐ¹ ÑÐ¿Ð¾ÑÐ¾Ð± ÑÑÐ°Ð½ÐµÐ½Ð¸Ñ ÐÐÐ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·ÑÐµÑÑÑ Ð´Ð»Ñ Ð½ÐµÐ¾Ð½Ð°ÑÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÐºÑÐ¸Ð½Ð¸Ð½Ð³Ð°. Ð¡ 31 Ð´ÐµÐºÐ°Ð±ÑÑ 2022 Ð³Ð¾Ð´Ð° Ð²ÑÑÐµÐ» Ð¿ÑÐ¸ÐºÐ°Ð· Ð¾ ÑÐ°ÑÑÐ¸ÑÐµÐ½Ð¸Ñ Ð½ÐµÐ¾Ð½Ð°ÑÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÐºÑÐ¸Ð½Ð¸Ð½Ð³Ð° Ð´Ð¾ 36 Ð³ÑÑÐ¿Ð¿ Ð½Ð¾Ð·Ð¾Ð»Ð¾Ð³Ð¸Ð¹. Ð ÑÐ²ÑÐ·Ð¸ Ñ ÑÑÐ¸Ð¼, Ð²Ð¾Ð·ÑÐ°ÑÑÐ°ÐµÑ Ð¿Ð¾ÑÑÐµÐ±Ð½Ð¾ÑÑÑ Ð² Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾ÑÑÐ¸ Ð°Ð²ÑÐ¾Ð¼Ð°ÑÐ¸Ð·Ð¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ ÐÐÐ ÑÐ¾ ÑÐ¿ÐµÑÐ¸Ð°Ð»ÑÐ½ÑÑ ÑÐµÑÑ- Ð±Ð»Ð°Ð½ÐºÐ¾Ð² Ð´Ð»Ñ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐµÐ³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð°. Ð­ÑÐ¾ Ð¿Ð¾Ð¼Ð¾Ð¶ÐµÑ ÑÐ¿ÑÐ¾ÑÑÐ¸ÑÑ Ð¿ÑÐ¾ÑÐµÑÑ ÑÐ°Ð±Ð¾ÑÑ ÑÐ¾ÑÑÑÐ´Ð½Ð¸ÐºÐ¾Ð² Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¹ Ð¸ ÑÑÐºÐ¾ÑÐ¸ÑÑ Ð¿ÑÐ¾ÑÐµÑÑ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð² Ð´Ð»Ñ ÑÐ²Ð¾ÐµÐ²ÑÐµÐ¼ÐµÐ½Ð½Ð¾-Ð³Ð¾ Ð¾Ð¿Ð¾Ð²ÐµÑÐµÐ½Ð¸Ñ Ð¾ Ð¿Ð°ÑÐ¾Ð»Ð¾Ð³Ð¸ÑÑ Ð¿Ð°ÑÐ¸ÐµÐ½ÑÐ° Ð¸ Ð½Ð°Ð·Ð½Ð°ÑÐµÐ½Ð¸Ñ ÐºÐ¾ÑÑÐµÐºÑÐ½Ð¾Ð³Ð¾ Ð»ÐµÑÐµÐ½Ð¸Ñ.
description: Research objective: to develop an extraction kit from dried blood spots based on adsorption on magnetic beads MagnoSP-NA-Bio (manufacturer: GC Alkor Bio, Rus-sia) Objectives: 1. Review of scientific literature related to the research topic 2. Selecting the optimum composition of the kit for separation from dried blood-stains based on sorption onto magnetic particles 3. To compare the extraction efficiency of dried blood spots with extraction kits from other manufacturers 4. Testing the kit on more samples. 5. Analyzing the results. The main methods used in the development of the kit were dry spot, nucleic acid extraction based on adsorption on magnetic beads, polymerase chain reaction (PCR) and concentration measurement by fluorescence measurement. Scopus, PubMed, cyberleninka.ru, nature.com and Elibrary.ru databases were used to prepare the literature search. Statistical processing of experimental data was performed using Microsoft Excel software package. Early detection of genetic abnormalities in newborns ensures timely prescription of effective treatments and prevents serious consequences. The dry spot method is a convenient way to preserve human DNA and is used for newborn screening; an order has been issued to extend newborn screening to 36 disease groups from December 31, 2022. As a result, there is a growing need to automatically extract DNA from special test forms and make it available for further analysis. This will facilitate the work of laboratory staff and speed up the process of obtaining results, thus informing them in time about the patients condition and enabling them to prescribe the right treatment.