Additional file 1 of Near-infrared light triggered in situ release of CO for enhanced therapy of glioblastoma
Creators: name: Ge, Juan
name: Zuo, Miaomiao
name: Wang, Qirong
name: Li, Zhen
Publisher: name: figshare
Year: 2025
Subjects: Biophysics
Space Science
Physical Sciences not elsewhere classified
Medicine
Pharmacology
Chemical Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Cancer

description: Additional file 1: Figure S1. 1H-NMR spectrum of 3HBQ. Figure S2. 13C-NMR spectrum of 3HBQ. Figure S3. High resolution mass spectrum (HRMS) of 3HBQ. Figure S4. 3HBQ solution light response releases CO. Figure S5. The fluorescence emission spectra of 3HBQ solution after being irradiated with a 450-nm LED light source. Figure S6. The mechanism of CO detecting by FL-CO. Figure S7. The fluorescence spectra of FL-CO incubated with 3HBQ and irradiated for different times in 50% DMSO-PBS buffer. Figure S8. HRMS of the product obtained by irradiating 3HBQ. Figure S9. The mechanism of CO release by 3HBQ. Figure S10. The histograms of the size distribution of (a) C, (b) C-S1, (c) C-S1-S2, (d) C-S1-S2-S3, (e) C-S1-S2-S3-S4 structured UCNPs. Figure S11. HRTEM image of OA-UCNPs. Figure S12. Constituent elements and contents of OA-UCNPs. Figure S13. Upconversion emission spectrum of UCNPs under 980-nm laser excitation. Figure S14. FTIR spectra of OA-UCNPs, PC and PC-UCNPs. Figure S15. The TEM image (a) and histograms of the size distribution (b) of UCNPs@Ce6/3HBQ. Figure S16. UV–vis absorption spectra of (a) Ce6 and (c) 3HBQ with different concentrations in DMSO. Figure S17. UV–visible absorption spectra of UCNPs@Ce6/3HBQ and the supernatant after centrifugation within 48 h. Figure S18. Detection of reactive oxygen species production by UCNPs@Ce6 and CO release by UCNPs@3HBQ. Figure S19. Control experiments to exclude the direct effect of NIR on 3HBQ and Ce6 molecules. Figure S20. UCNPs@Ce6/3HBQ stability test. Figure S21. UCNPs@Ce6/3HBQ cell uptake imaging. Figure S22. Expression levels of HMOX-1, AKT-1 and NRF-2 in U87MG cells after receiving different treatments. Figure S23. ATP levels in U87MG cells after receiving different treatments. Figure S24. Expression levels of TNF-α and IL-6 in U87MG cells after receiving different treatments. Figure S25. Zeta potential of 1) CM, 2) UCNPs@Ce6/3HBQ and 3) UCNPs@Ce6/3HBQ@CM. Figure S26. Hydrodynamic diameter of UCNPs@Ce6/3HBQ and UCNPs@Ce6/3HBQ@CM. Figure S27. The TEM image and histogram of the size distribution of UCNPs@Ce6/3HBQ@CM. Figure S28. SDS-PAGE protein analysis and western blot analysis of UCNPs@Ce6/3HBQ@CM. Figure S29. Western blot analysis and TEM images of UCNPs@Ce6/3HBQ@CM. Figure S30. H&amp;E staining of major organs (healthy mice) after injection with different dosages of UCNPs@Ce6/3HBQ@CM. Figure S31. Blood routine and serum biochemical levels of mice after injection with different doses of UCNPs@Ce6/3HBQ@CM. Figure S32. Blood concentration–time curve of UCNPs@Ce6/3HBQ@CM in mice. Figure S33. Y3+ content in major organs of mice after injection of UCNPs@Ce6/3HBQ@CM for 7 days and 14 days. Figure S34. HMOX-1 and NRF-2 levels of GBM site on the 15th day. Figure S35. TNF-α and IL-6 levels of GBM site on the 15th day. Figure S36. H&amp;E stained major organs of U87MG-Luc glioma-bearing mice after receiving different treatments.