title: ÐÑÐ¾Ð±ÐµÐ½Ð½Ð¾ÑÑÐ¸ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Ð°ÑÑÑÐ¾ÑÐ¸ÑÐ°ÑÐ½ÑÑ Ð±ÐµÐ»ÐºÐ¾Ð² Ð² ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð¼Ð¾Ð´ÐµÐ»ÑÑ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Changes in the expression of astrocyte protein genes in various experimental models of epilepsy
Creators: name: ÐÐ°ÑÐ°ÑÐ¾Ð²Ð°, ÐÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: GFAP
EAAT2
IP3R2
Il-1Î²
TNFÎ±
ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ñ
Ð¿ÐµÐ½ÑÐ¸Ð»ÐµÐ½ÑÐµÑÑÐ°Ð·Ð¾Ð»Ð¾Ð²Ð°Ñ Ð¼Ð¾Ð´ÐµÐ»Ñ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÑÐ°ÑÑÑÐ°
ÑÐµÐ±ÑÐ¸Ð»ÑÐ½ÑÐµ ÑÑÐ´Ð¾ÑÐ¾Ð³Ð¸
Ð²Ð¸ÑÐ¾ÑÐ½Ð°Ñ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ñ
Ð»Ð¸ÑÐ¸Ð¹-Ð¿Ð¸Ð»Ð¾ÐºÐ°ÑÐ¿Ð¸Ð½Ð¾Ð²Ð°Ñ Ð¼Ð¾Ð´ÐµÐ»Ñ
epilepsy
pentilentetrazole model of status epilepticus
febrile seizure
temporal lobe epilepsy
lithium-pilocarpine model

description: Ð Ð°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐ¸ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Ð°ÑÑÑÐ¾ÑÐ¸ÑÐ°ÑÐ½ÑÑ Ð±ÐµÐ»ÐºÐ¾Ð² GFAP, IP3R2, EAAT2, IL-1Î² Ð¸ TNFÎ± Ð² ÐºÐ»ÐµÑÐºÐ°Ñ Ð¼Ð¾Ð·Ð³Ð° ÐºÑÑÑ Ð² ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð¼Ð¾Ð´ÐµÐ»ÑÑ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ð¸. Ð Ð°Ð±Ð¾ÑÐ° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ ÐÐ°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½ÑÑ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð¾Ð² Ð½ÐµÐ¹ÑÐ¾Ð½Ð½ÑÑ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ð¹ ÐÐ­Ð¤Ð Ð ÐÐ. ÐÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð¿ÐµÐ½ÑÐ¸Ð»ÐµÐ½ÑÐµÑÑÐ°Ð·Ð¾Ð»-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÑÐ°ÑÑÑÐ° (ÐÐ¢Ð), ÑÐµÐ±ÑÐ¸Ð»ÑÐ½ÑÑ ÑÑÐ´Ð¾ÑÐ¾Ð³ (Ð¤Ð¡), Ð»Ð¸ÑÐ¸Ð¹-Ð¿Ð¸Ð»Ð¾ÐºÐ°ÑÐ¿Ð¸Ð½Ð¾Ð²Ð°Ñ Ð¼Ð¾Ð´ÐµÐ»Ñ Ð²Ð¸ÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ð¸ (ÐÐ). ÐÐ½Ð°Ð»Ð¸Ð· ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Gfap, Itpr2, Slc1a2 (Ð±ÐµÐ»Ð¾Ðº - EAAT2), Il-1Î² Ð¸ TnfÎ± Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»Ð¸ Ð¼ÐµÑÐ¾Ð´Ð°Ð¼Ð¸ ÐÐ¢-ÐÐ¦Ð  Ð² ÑÐµÐ°Ð»ÑÐ½Ð¾Ð¼ Ð²ÑÐµÐ¼ÐµÐ½Ð¸ Ð¸ Ð²ÐµÑÑÐµÑÐ½ Ð±Ð»Ð¾ÑÑÐ¸Ð½Ð³Ð°. Ð ÐÐ¢Ð Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð¾ÑÑÑÐ¾Ð³Ð¾ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÑÐ°ÑÑÑÐ° Ð²ÑÑÐ²Ð»ÐµÐ½Ð¾ ÑÑÐ¸Ð»ÐµÐ½Ð¸Ðµ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Gfap, Itpr2, TnfÎ± Ð¸ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ðµ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Slc1a2 (EAAT2). ÐÐ·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð±Ð¾Ð»ÐµÐµ Ð²ÑÑÐ°Ð¶ÐµÐ½Ñ Ð² Ð¿ÐµÑÐ²ÑÐµ Ð´Ð½Ð¸ Ð¿Ð¾ÑÐ»Ðµ ÑÑÐ´Ð¾ÑÐ¾Ð³. Ð ÐÐ Ð¼Ð¾Ð´ÐµÐ»Ð¸ ÑÑÐ¾Ð½Ð¸ÑÐµÑÐºÐ¾Ð¹ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ð¸ ÑÑÐ¸Ð»ÐµÐ½Ð¸Ðµ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Ð³ÐµÐ½Ð¾Ð² Gfap, Itpr2, TnfÎ±, Ð½Ð°Ð±Ð»ÑÐ´Ð°ÐµÑÑÑ Ð²Ð¾ Ð²ÑÐµÑ Ð¾Ð±ÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð½ÑÑ Ð¾Ð±Ð»Ð°ÑÑÑÑ Ð¼Ð¾Ð·Ð³Ð°, Ð´Ð»Ñ Gfap Ð¾Ð½Ð¸ Ð½Ð¾ÑÑÑ Ð´Ð¾Ð»Ð³Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½ÑÐ¹ ÑÐ°ÑÐ°ÐºÑÐµÑ. ÐÐ¾ÑÐ»Ðµ Ð¤Ð¡ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ (ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ðµ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Slc1a2, Gfap, Itpr2 Ð¸ TnfÎ±) Ð±Ð¾Ð»ÐµÐµ Ð²ÑÑÐ°Ð¶ÐµÐ½Ñ Ð² Ð´Ð¾ÑÐ·Ð°Ð»ÑÐ½Ð¾Ð¼ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ðµ, ÐºÑÐ¾Ð¼Ðµ ÑÐ¾Ð³Ð¾, Ñ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ ÐºÑÑÑ Ð½Ð°Ð±Ð»ÑÐ´Ð°Ð»Ð¾ÑÑ Ð½Ð°ÑÑÑÐµÐ½Ð¸Ðµ Ð²Ð¾Ð·ÑÐ°ÑÑÐ½Ð¾Ð¹ Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐ¸ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Slc1a2 Ð¸ Gfap. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ Ð´Ð°Ð½Ð½ÑÐµ Ð¼Ð¾Ð³ÑÑ Ð±ÑÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ Ð¿ÑÐ¸ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐµ Ð½Ð¾Ð²ÑÑ Ð¿ÑÐ¾ÑÐ¸Ð²Ð¾ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð².
description: The work is devoted to study the dynamics of the astrocyte protein genes (GFAP, IP3R2, EAAT2, IL-1Î², and TNFÎ±) expression in rat brain in experimental models of epilepsy.The study was performed in the Laboratory of molecular mechanisms of neural interactions, IEPhB RAS. We used models of pentylenetetrazole-induced status epilepticus (PTZ), febrile seizures (FS), and a lithium-pilocarpine model of temporal lobe epilepsy (PC). Analysis of Gfap, Itpr2, Slc1a2 (protein - EAAT2), Il-1Î², and TnfÎ± gene expression was carried out by real-time RT-PCR and Western blot. In the PTZ model of acute status epilepticus, an increase in the expression of the Gfap, Itpr2, TnfÎ± genes, and a decrease in the expression of Slc1a2 (EAAT2) were revealed. The changes were more pronounced in the first days after seizures. Increased Gfap, Itpr2, TnfÎ± gene expression was observed in all examined brain regions in the PC model of chronic epilepsy. The changes in Gfap expression were long-term. After FS we found decreased expression of the Slc1a2, Gfap, Itpr2, and TnfÎ± genes; the changes were more pronounced in the dorsal hippocampus. Moreover, a disruption of the age dynamics of the Slc1a2 and Gfap genes expression was observed in experimental rats. The obtained data can be used in the development of new antiepileptic drugs.