Characterization of HIV-1 pol sequences in newly HIV-1 diagnosed patients: identification of drug resistance markers and their involvement in the transmission events
Creators: name: ALTERI, CLAUDIA
Publisher: name: Università degli Studi di Roma "Tor Vergata"
Year: 2010
Subjects: subject: HIV-1; drug resistance markers; transmission of drug resistance; drug naïve patients
subject: HIV-1
subject: drug resistance markers
subject: transmission of drug resistance
subject: drug naïve patients

description: 22. ciclo
description: 1 Introduction 1.1 HIV and the retroviruses 1.1.1 Morphology 1.1.2 Genome 1.1.3 Replication 1.1.4 Pathogenesis 1.1.5 Epidemiology 1.2 Drug resistance development 1.2.1 Antiretroviral treatment 1.2.2 Drug resistance 1.2.3 Mechanism of drug resistance 1.2.4 Combination therapy 1.2.5 Detection of drug resistance 1.3 Transmission of HIV-1 drug resistance 1.3.1 Evolution of drug resistance in treatment naïve patients 1.3.2 Mechanism of transmission of drug resistant virus 1.3.3 Estimation rate of transmission of drug resistant virus 1.3.4 Clinical implications of transmitted drug resistance 1.4 Rationale of the work. - 2 Methods 2.1 Characterization of the patterns of drug resistance mutations in newly diagnosed HIV-1 infected patients naïve to the antiretroviral drugs 2.1.1 Study population 2.1.2 The IgG avidity assay 2.1.3 HIV sequencing 2.1.4 Phylogenetic analysis 2.1.5 Determination of drug resistance mutations 2.1.6 Statistical analysis 2.2 Epidemiological network analysis in HIV-1 B infected patients diagnosed in Italy between 2000-2008 2.2.1 Study population 2.2.2 HIV sequencing 2.2.3 Phylogenetic analysis 2.2.4 Genotypic resistance analysis 2.2.5 Time-scaled phylogenies 2.3 “Sentinel” mutations in standard population sequencing can predict the presence of RT major mutations detected only by 454-pyrosequencing 2.3.1 Study population 2.3.2 HIV sequencing 2.3.3 Determination of drug resistance minority variants 2.3.4 Statistical analysis. - 3 Results 3.1 Characterization of the patterns of drug resistance mutations in newly diagnosed HIV-1 infected patients naïve to the antiretroviral drugs 3.1.1 Study population 3.1.2 Circulation of HIV-1 subtypes in newly diagnosed patients in Italy 3.1.3 Prevalence of major mutations associated with drug resistance 3.1.4 Association among drug resistance mutations in newly diagnosed HIV-1 infected patients 3.2 Epidemiological network analysis in HIV-1 B infected patients diagnosed in Italy between 2000-2008 3.2.1 Study population 3.2.2 Phylogenetic analysis 3.2.3 Identification of antiretroviral drug resistant HIV-1 lineages in drug naïve patients 3.2.4 Description of the major lineages 3.2.5 Timing of origin of the major lineages 3.3 “Sentinel” mutations in standard population sequencing can predict the presence of RT major mutations detected only by 454-pyrosequencing 3.3.1 Study population 3.3.2 Coverage profile and UDPS results 3.3.3 Role of atypical RT mutations in predicting minority resistance variants 3.3.4 Response to HAART. - 4 Discussion 4.1 Characterization of the patterns of drug resistance mutations in newly diagnosed HIV-1 infected patients naïve to the antiretroviral drugs 4.2 Epidemiological network analysis in HIV-1 B infected patients diagnosed in Italy between 2000-2008 4.3 “Sentinel” mutations in standard population sequencing can predict the presence of RT major mutations detected only by 454-pyrosequencing . - 5 Conclusion