title: ÐÐ·ÑÑÐµÐ½Ð¸Ðµ Ð½ÐµÐºÐ°Ð½Ð¾Ð½Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð½ÐµÐ²Ð¸ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ Ð ÐÐ-Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ñ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð° phiKZ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ° Ð¿Ð¾ Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð¸Ñ 03.04.02 - Ð¤Ð¸Ð·Ð¸ÐºÐ° ; 03.04.02_02 - ÐÐ¸Ð¾ÑÐ¸Ð·Ð¸ÐºÐ°
titleType: Subtitle
Creators: name: ÐÑÐµÑÐ¾Ð²Ð°, ÐÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2019
Subjects: ÐÐÐ-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð°Ñ Ð ÐÐ-Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð°
Ð¼Ð½Ð¾Ð³Ð¾ÑÑÐ±ÑÐµÐ´Ð¸Ð½Ð¸ÑÐ½Ð°Ñ Ð½ÐµÐºÐ°Ð½Ð¾Ð½Ð¸ÑÐµÑÐºÐ°Ñ Ð ÐÐ-Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð°
Ð½ÐµÐ²Ð¸ÑÐ¸Ð¾Ð½Ð½Ð°Ñ Ð ÐÐ-Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð°
ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸Ñ
Ð³Ð¸Ð³Ð°Ð½ÑÑÐºÐ¸Ð¹ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³ phiKZ
Ð±ÐµÐ»Ð¾Ðº-Ð±ÐµÐ»ÐºÐ¾Ð²ÑÐµ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ
ÐÐÐ-Ð±ÐµÐ»ÐºÐ¾Ð²ÑÐµ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð½ÐµÐºÐ°Ð½Ð¾Ð½Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð½ÐµÐ²Ð¸ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÐÐÐ-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾Ð¹ Ð ÐÐ-Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ñ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð° phiKZ, Ð² ÑÐ°ÑÑÐ½Ð¾ÑÑÐ¸ Ð²Ð½Ð¸Ð¼Ð°-Ð½Ð¸Ðµ Ð±ÑÐ»Ð¾ ÑÐ´ÐµÐ»ÐµÐ½Ð¾ ÑÐ±Ð¾ÑÐºÐµ Ð°ÐºÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ° Ð½ÐµÐ²Ð¸ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ Ð ÐÐÐ Ð´Ð¾ Ð½Ð°ÑÐ°-Ð»Ð° ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸Ð¾Ð½Ð½Ð¾Ð³Ð¾ ÑÐ¸ÐºÐ»Ð°. ÐÐµÐ²Ð¸ÑÐ¸Ð¾Ð½Ð½Ð°Ñ Ð ÐÐÐ ÑÐ¾ÑÑÐ¾Ð¸Ñ Ð¸Ð· 5 ÑÑÐ±ÑÐµÐ´Ð¸Ð½Ð¸Ñ, 4 Ð¸Ð· ÐºÐ¾ÑÐ¾ÑÑÑ ÑÐ²Ð»ÑÑÑÑÑ Ð³Ð¾Ð¼Ð¾Ð»Ð¾Ð³Ð°Ð¼Ð¸ Ð±Ð¾Ð»ÑÑÐ¸Ñ ÐºÐ°ÑÐ°Ð»Ð¸ÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÑÐ±ÑÐµÐ´Ð¸Ð½Ð¸Ñ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð¹ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ñ, Ð° Ð¿ÑÑÐ°Ñ ÑÑÐ±ÑÐµÐ´Ð¸Ð½Ð¸ÑÐ° Ð½Ðµ Ð¸Ð¼ÐµÐµÑ Ð³Ð¾Ð¼Ð¾Ð»Ð¾Ð³Ð¾Ð² Ñ Ð¸Ð·-Ð²ÐµÑÑÐ½Ð¾Ð¹ ÑÑÐ½ÐºÑÐ¸ÐµÐ¹. ÐÑÐ»Ð¾ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¾, ÑÑÐ¾ Ð¿ÑÐ¸ÑÑÑÑÑÐ²Ð¸Ðµ Ð¿ÑÑÐ¾Ð¹ ÑÑÐ±ÑÐµÐ´Ð¸Ð½Ð¸ÑÑ Ð² ÐºÐ»ÐµÑÐºÐµ Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼Ð¾ Ð´Ð»Ñ ÑÐ±Ð¾ÑÐºÐ¸ Ð°ÐºÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ°. Ð¢Ð°ÐºÐ¶Ðµ Ð±ÑÐ»Ð¾ Ð¾ÑÐ¼ÐµÑÐµÐ½Ð¾, ÑÑÐ¾ ÑÐ±Ð¾ÑÐºÐ° ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ° Ð½Ð°ÑÐ¸Ð½Ð°ÐµÑÑÑ Ñ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ ÑÑÐ±ÑÐµÐ´Ð¸Ð½Ð¸Ñ, Ð¾Ð±ÑÐ°Ð·ÑÑ-ÑÐ¸Ñ Ð¾ÑÐ½Ð¾Ð²Ð½ÑÑ ÑÐ°ÑÑÑ Ð°ÐºÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÐµÐ½ÑÑÐ° Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ñ, Ð° Ð¸Ñ ÑÐ¸ÐºÑÐ°ÑÐ¸Ñ Ð² Ð¿ÑÐ°-Ð²Ð¸Ð»ÑÐ½Ð¾Ð¹ ÐºÐ¾Ð½ÑÐ¾ÑÐ¼Ð°ÑÐ¸Ð¸, Ð¿Ð¾-Ð²Ð¸Ð´Ð¸Ð¼Ð¾Ð¼Ñ, Ð¿ÑÐ¾Ð¸ÑÑÐ¾Ð´Ð¸Ñ Ð¿ÑÐ¸ Ð¿Ð¾Ð¼Ð¾ÑÐ¸ Ð¿ÑÑÐ¾Ð¹ ÑÑÐ±Ñ-ÐµÐ´Ð¸Ð½Ð¸ÑÑ. ÐÐ¾Ð¿Ð¾Ð»Ð½Ð¸ÑÐµÐ»ÑÐ½Ð¾ Ð±ÑÐ»Ð¸ Ð²ÑÑÐ²Ð»ÐµÐ½Ñ ÑÑÐ±ÑÐµÐ´Ð¸Ð½Ð¸ÑÑ Ð½ÐµÐ²Ð¸ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ Ð ÐÐÐ, ÐºÐ¾ÑÐ¾ÑÑÐµ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²ÑÑÑ Ñ ÐÐÐ.
description: This work is devoted to the study of non-canonical multisubunit non-virion DNA-dependent RNA-polymerase encoded by giant bacteriophage phiKZ. The main topic of presented work was the assembly of the active complex non-virion RNAP be-fore the transcription cycle begins. The non-virion RNAP consists of 5 subunits, 4 of which are homologs of the large catalytic subunits of bacterial polymerase, and the fifth subunit has no homologs with a known function. It was determined that the presence of the fifth subunit in the cell is necessary for the assembly of the ac-tive complex. It was also noted that the assembly of the complex begins with the interaction of subunits forming the main part of the polymerase active center, and it seems that their fixation in the correct conformation occurs with the help of the fifth subunit. Additionally, subunits of non-virion RNAP that interact with DNA were identified.