BNT162b2 Vaccine: possible codons misreading, errors in protein synthesis and alternative splicing's anomalies
Creators: name: Smith, Kira
Publisher: name: Zenodo
Year: 2021
Subjects: Covid
Covid-19
Vaccine
BNT162b2
Alternative splicing
Misreading
Codons
Codon
Codon bias
Wobble
Translation
Errors
Protein synthesis
Harmful
Long-Term
Safety
Vaccines safety
Vaccines
Covid Vaccine
Covid-19 vaccine
Vaccine Covid

description: <strong>BNT162b2</strong> vaccine against <strong>Covid-19</strong> is composed of an <strong>RNA</strong> having <strong>4284 nucleotides</strong>, divided into <strong>6 sections</strong>, which bring the <strong>information</strong> to create a <strong>factory of S Spike proteins</strong>, the ones used by <strong>Sars-CoV-2 (Covid-19)</strong> to <strong>infect</strong> the host. After that, these <strong>proteins</strong> are directed <strong>outside the cell</strong>, triggering the <strong>immune reaction</strong> and <strong>antibody</strong> production. The problem is the <strong>heavy alteration of the mRNA</strong>: <strong>Uracil</strong> is replaced to fool the immune system with <strong>Ψ</strong><strong> </strong>(<strong>Pseudouridine</strong>); the <strong>letters of all codon triplets</strong> are replaced by a <strong>C</strong> or a <strong>G</strong>, to <strong>extremely increase </strong>the<strong> speed </strong>of <strong>protein synthesis</strong>; replacement of some <strong>amino acids</strong> with <strong>Proline</strong>; addition of a <strong>sequence (3'-UTR)</strong> with <strong>unknown</strong> alteration. These <strong>impairments</strong> could cause strong <strong>doubts</strong> about the presence of <strong>codon usage errors</strong>. An eventual<strong> </strong><strong>mistranslation</strong> has consequences on the <strong>pathophysiology</strong> of a <strong>variety of diseases</strong>.<br> In addition, <strong>mRNA injected</strong> is a <strong>pre-mRNA</strong>, which can <strong>lead</strong> to the <strong>multiple</strong> mature <strong>mRNAs</strong>; these are <strong><em>alternative splicing</em></strong> <strong>anomalies</strong>, direct <strong>source</strong> of serious <strong>long-term harm</strong> on the human health. In essence, what will be created <strong>may not be identical</strong> with <strong>protein S Spike</strong>: just an <strong>error in translational decoding</strong>, codons<strong> misreading</strong>, production of <strong>different</strong> amino acids, then <strong>proteins</strong>, to cause serious <strong>long-term damage</strong> to human <strong>health</strong>, despite the <strong>DNA is not modified</strong>, being instead in the <strong>cell nucleus</strong> and <strong>not in the cytoplasm</strong>, where the modified mRNA arrives. However, in this case, the <strong>correlation</strong> between <strong>speed of synthesis</strong> and <strong>protein expression</strong> with <strong>synthesis errors</strong>, as well as the <strong>mechanism</strong> that could affect the <strong>translation of the sequence</strong> remain obscure, many <strong>trials</strong> have <strong>not yet</strong> been <strong>performed</strong>.
description: Published on Journal of Antivirals and Andtiretrovirals by Longdom