title: ÐÐ¿ÑÐ¸Ð¼Ð¸Ð·Ð°ÑÐ¸Ñ ÑÐµÐ¶Ð¸Ð¼Ð¾Ð² ÑÐ°Ð±Ð¾ÑÑ ÑÐµÐºÐ²ÐµÐ½Ð°ÑÐ¾ÑÐ° ÐÐÐÐÐ¤ÐÐ -Ð¡ÐÐ¡
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Optimization of the operation modes of the sequenator nanofor SPS
Creators: name: ÐÐ¸Ð½Ð°ÐºÐ¾Ð², ÐÐµÐ½Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: ÑÐµÐºÐ²ÐµÐ½Ð°ÑÐ¾Ñ
ÐÐ°Ð½Ð¾ÑÐ¾Ñ Ð¡ÐÐ¡
Ð¾Ð¿ÑÐ¸Ð¼Ð¸Ð·Ð°ÑÐ¸Ñ
ÑÐ»ÐµÐ¼ÐµÐ½Ñ ÐÐµÐ»ÑÑÑÐµ
Ð³Ð¸Ð´ÑÐ°Ð²Ð»Ð¸ÑÐµÑÐºÐ°Ñ ÑÐ¸ÑÑÐµÐ¼Ð°
sequencer
Nanofor SPS
operating modes
optimization
hydraulic system
Pelte element

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description: The given work is devoted to the study of the modules of the NANOFOR SPS sequencer. To optimize the operation of the hydraulic system, the temperature control system of the cell and the thermal cycler, the following software was developed: âWeight Testâ, âRefrigerator Testâ, âThermocycler Testâ. For each program, a graphical interface was developed and written, allowing a person without special skills to use tests and check the correct operation of modules. With the help of the developed software, the above modules were tested. According to the results of the work, it was found that, for a hydraulic system, the fluid pumping speed does not affect the error in the fluid pumping volume, and also that, regardless of the fluid volume pumped, the error also remains within the limits declared by the manufacturer. This means that the hydraulic system is functioning properly. The temperature modules of the device were also tested and, as a result of the work, the optimal temperature coefficients were selected, both for the temperature control system of the cell and for the thermal cycler. This reduced the temperature gap between the built-in temperature sensors and the external precision thermometer to 0.1 Â°C. An error was also identified in the operation of the cell temperature control system, in which moisture entered the analog circuit of the temperature sensor, due to which the sensor showed a temperature of -100 Â°C and the cell temperature control system turned off. The results of this work are improved temperature performance of temperature modules. This affected both the safety of the reagents used in the operation of the sequencer and the accuracy of cell heating at different stages of the operation of the NANOFOR SPS sequencer.