title: ÐÐ½Ð°Ð»Ð¸Ð· Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹ Ð² Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð°ÐºÑÐ¸Ð½Ð¾Ð²Ð¾Ð³Ð¾ ÑÐ¸ÑÐ¾ÑÐºÐµÐ»ÐµÑÐ° ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ Ð»Ð¸Ð½Ð¸Ð¸ FetMSC Ð² Ð¿ÑÐ¾ÑÐµÑÑÐµ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Analysis of changes in the organization of the actin cytoskeleton of the FetMSC cell line during replicative senescence
Creators: name: ÐÐ¾ÑÐ±, ÐÐ½Ð°ÑÑÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÐµ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸
FetMSC
Ð°ÐºÑÐ¸Ð½Ð¾Ð²ÑÐ¹ ÑÐ¸ÑÐ¾ÑÐºÐµÐ»ÐµÑ
ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ðµ ÑÑÐ°ÑÐµÐ½Ð¸Ðµ
RhoA
Rac1
mesenchymal stem cells
actin cytoskeleton
replicative senescence
GTPase RhoA
GTPase Rac1

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸Ðº, ÑÐ²ÑÐ·Ð°Ð½Ð½ÑÑ Ñ Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð°ÑÐ¸ÐµÐ¹ Ð°ÐºÑÐ¸Ð½Ð¾Ð²Ð¾Ð³Ð¾ ÑÐ¸ÑÐ¾ÑÐºÐµÐ»ÐµÑÐ°, Ð² Ð¿ÑÐ¾ÑÐµÑÑÐµ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ Ð»Ð¸Ð½Ð¸Ð¸ FetMSC ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: 1. ÐÑÐ°ÑÐ°ÐºÑÐµÑÐ¸Ð·Ð¾Ð²Ð°ÑÑ Ð¿ÑÐ¾ÑÐµÑÑ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð¾ÑÐµÐ½ÐºÐ¸ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹ ÐºÐ»ÐµÑÐ¾Ðº FetMSC Ð² Ð¿ÑÐ¾ÑÐµÑÑÐµ Ð´Ð»Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ; 2. ÐÑÐµÐ½Ð¸ÑÑ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð°ÐºÑÐ¸Ð½Ð¾Ð²Ð¾Ð³Ð¾ ÑÐ¸ÑÐ¾ÑÐºÐµÐ»ÐµÑÐ° ÐºÐ»ÐµÑÐ¾Ðº FetMSC Ð² Ð¿ÑÐ¾ÑÐµÑÑÐµ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ; 3. ÐÑÐµÐ½Ð¸ÑÑ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ ÑÐ´ÐµÑÐ½Ð¾Ð¹ Ð¸ ÑÐ¸ÑÐ¾Ð¿Ð»Ð°Ð·Ð¼Ð°ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ RhoA Ð¸ Rac1 Ð² ÐºÐ»ÐµÑÐºÐ°Ñ FetMSC Ð² Ð¿ÑÐ¾ÑÐµÑÑÐµ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð¿ÑÐ¾Ð´ÐµÐ»Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¾ Ð²ÑÑÑÐ½ÐµÐ½Ð¾, ÑÑÐ¾: 1. ÐÐ»ÐµÑÐ¾ÑÐ½Ð°Ñ Ð»Ð¸Ð½Ð¸Ñ FetMSC Ð¿ÑÐ¾ÑÐ²Ð»ÑÐµÑ Ð¿ÑÐ¸Ð·Ð½Ð°ÐºÐ¸ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ Ð² Ð¿ÑÐ¾ÑÐµÑÑÐµ Ð´Ð»Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ; 2. Ð² Ð¿ÑÐ¾ÑÐµÑÑÐµ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ ÐºÐ»ÐµÑÐ¾Ðº FetMSC Ð½Ð°Ð±Ð»ÑÐ´Ð°ÐµÑÑÑ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ðµ ÑÐ´ÐµÑÐ½Ð¾Ð¹ Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ ÐÐ¢Ð¤Ð°Ð· RhoA Ð¸ Rac1, ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ðµ ÐºÐ¾Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ Rac1 Ñ F-Ð°ÐºÑÐ¸Ð½Ð¾Ð¼, ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ðµ ÐºÐ¾Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ RhoA Ñ F-Ð°ÐºÑÐ¸Ð½Ð¾Ð¼ Ð½Ð° ÑÐ°Ð½Ð½Ð¸Ñ Ð¿Ð°ÑÑÐ°Ð¶Ð°Ñ Ð¸ Ð¿Ð¾Ð²ÑÑÐµÐ½Ð¸Ðµ Ð½Ð° Ð¿Ð¾Ð·Ð´Ð½Ð¸Ñ Ð¿Ð°ÑÑÐ°Ð¶Ð°Ñ; 3. Ð² Ð¿ÑÐ¾ÑÐµÑÑÐµ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ ÐºÐ»ÐµÑÐ¾Ðº FetMSC Ð½Ð°Ð±Ð»ÑÐ´Ð°ÐµÑÑÑ ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ðµ Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¾Ð²Ð°Ð½Ð½Ð¾ÑÑÐ¸ Ð°ÐºÑÐ¸Ð½Ð¾Ð²Ð¾Ð³Ð¾ ÑÐ¸ÑÐ¾ÑÐºÐµÐ»ÐµÑÐ° Ð½Ð° Ð¿Ð¾Ð·Ð´Ð½Ð¸Ñ Ð¿Ð°ÑÑÐ°Ð¶Ð°Ñ.
description: This work is devoted to the study of changes in characteristics associated with the organization of the actin cytoskeleton during replicative senescence of the FetMSC cell line. The goals of the study are: 1. to characterize the process of replicative senescence by evaluation of morphological changes in FetMSC cells during long-term cultivation; 2. to evaluate the morphological changes in the actin cytoskeleton of FetMSC cells during replicative senescence; 3. to evaluate the changes in nuclear and cytoplasmic localization of RhoA and Rac1 in FetMSC cells during replicative senescence. As a result of this work it was found that: 1. FetMSC cell line exhibits replicative senescence during long-term cultivation; 2. During replicative senescence of FetMSC cells, a decrease in nuclear localization of RhoA and Rac1 GTPases, a decrease in Rac1 colocalization with F-actin, a decrease in RhoA colocalization with F-actin in early passages and an increase in late passages were observed; 3. During replicative senescence of FetMSC cells, an increase in actin cytoskeleton organization in late passages is observed.