Fusarium chuoi Crous, Osieck, Jurjevi, Boers, Iperen, Starink-Willemse, Dima, Balashov, Bulgakov, Johnston, Morozova, Pinruan, Sommai, Alvarado, Decock, Lebel, McMullan-Fisher, Moreno, Shivas, Zhao, Abdollahzadeh, Abrinbana, Ageev, Akhmetova, Alexandrova, Altés, Amaral, Angelini, Antonín, Arenas, Asselman, Badali, Baghela, Bañares, Barreto, Baseia, Bellanger, Berraf-Tebbal, Biketova, Bukharova, Burgess, Cabero, Câmara, Cano-Lira, Ceryngier, Chávez, Cowan, Lima, Oliveira, Denman, Dang, Dovana, Duarte, Eichmeier, Erhard, Esteve-Raventós, Fellin, Ferisin, Ferreira, Ferrer, Finy, Gaya, Geering, Gil-Durán, Glässnerová, Glushakova, Gramaje, Guard, Guarnizo, Haelewaters, Halling, Hill, Hirooka, Hubka, Iliushin, Ivanova, Ivanushkina, Jangsantear, Justo, Kachalkin, Kato, Khamsuntorn, Kirtsideli, Knapp, Kochkina, Koukol, Kovács, Kruse, Kumar, Kušan, Læssøe, Larsson, Lebeuf, Levicán, Loizides, Marinho, Luangsa-ard, Lukina, Magaña-Dueñas, Maggs-Kölling, Malysheva, Malysheva, Martín, Martín, Matočec, McTaggart, Mehrabi-Koushki, Mešić, Miller, Mironova, Moreau, Morte, Müller, Nagy, Nanu, Navarro-Ródenas, Nel, Nguyen, Nóbrega, Noordeloos, Olariaga, Overton, Ozerskaya, Palani, Pancorbo, Papp, Pawłowska, Pham, Phosri, Popov, Portugal, Pošta, Reschke, Reul, Ricci, Rodríguez, Romanowski, Ruchikachorn, Saar, Safi, Sakolrak, Salzmann, Sandoval-Denis, Sangwichein, Sanhueza, Sato, Sastoque, Senn-Irlet, Shibata, Siepe, Somrithipol, Spetik, Sridhar, Stchigel, Stuskova, Suwannasai, Tan, Thangavel, Tiago, Tiwari, Tkalčec, Tomashevskaya, Tonegawa, Tran, Tran, Trovão, Trubitsyn, Wyk, Vieira, Vila, Visagie, Vizzini, Volobuev, Vu, Wangsawat, Yaguchi, Ercole, Ferreira, Souza, Vieira &amp; Groenewald, 2021, sp. nov.
Creators: name: Crous, P. W.
name: Osieck, E. R.
name: Jurjevi, Ž
name: Boers, J.
name: Van Iperen, A. L.
name: Starink-Willemse, M.
name: Dima, B.
name: Balashov, S.
name: Bulgakov, T. S.
name: Johnston, P. R.
name: Morozova, O. V.
name: Pinruan, U.
name: Sommai, S.
name: Alvarado, P.
name: Decock, C. A.
name: Lebel, T.
name: McMullan-Fisher, S.
name: Moreno, G.
name: Shivas, R. G.
name: Zhao, L.
name: Abdollahzadeh, J.
name: Abrinbana, M.
name: Ageev, D. V.
name: Akhmetova, G.
name: Alexandrova, A. V.
name: Altés, A.
name: Amaral, A. G. G.
name: Angelini, C.
name: Antonín, V.
name: Arenas, F.
name: Asselman, P.
name: Badali, F.
name: Baghela, A.
name: Bañares, A.
name: Barreto, R. W.
name: Baseia, I. G.
name: Bellanger, J. - M.
name: Berraf-Tebbal, A.
name: Biketova, A. Yu.
name: Bukharova, N. V.
name: Burgess, T. I.
name: Cabero, J.
name: Câmara, M. P. S.
name: Cano-Lira, J. F.
name: Ceryngier, P.
name: Chávez, R.
name: Cowan, D. A.
name: de Lima, A. F.
name: Oliveira, R. L.
name: Denman, S.
name: Dang, Q. N.
name: Dovana, F.
name: Duarte, I. G.
name: Eichmeier, A.
name: Erhard, A.
name: Esteve-Raventós, F.
name: Fellin, A.
name: Ferisin, G.
name: Ferreira, R. J.
name: Ferrer, A.
name: Finy, P.
name: Gaya, E.
name: Geering, A. D. W.
name: Gil-Durán, C.
name: Glässnerová, K.
name: Glushakova, A. M.
name: Gramaje, D.
name: Guard, F. E.
name: Guarnizo, A. L.
name: Haelewaters, D.
name: Halling, R. E.
name: Hill, R.
name: Hirooka, Y.
name: Hubka, V.
name: Iliushin, V. A.
name: Ivanova, D. D.
name: Ivanushkina, N. E.
name: Jangsantear, P.
name: Justo, A.
name: Kachalkin, A. V.
name: Kato, S.
name: Khamsuntorn, P.
name: Kirtsideli, I. Y.
name: Knapp, D. G.
name: Kochkina, G. A.
name: Koukol, O.
name: Kovács, G. M.
name: Kruse, J.
name: Kumar, T. K. A.
name: Kušan, I.
name: Læssøe, T.
name: Larsson, E.
name: Lebeuf, R.
name: Levicán, G.
name: Loizides, M.
name: Marinho, P.
name: Luangsa-ard, J. J.
name: Lukina, E. G.
name: Magaña-Dueñas, V.
name: Maggs-Kölling, G.
name: Malysheva, E. F.
name: Malysheva, V. F.
name: Martín, B.
name: Martín, M. P.
name: Matočec, N.
name: McTaggart, A. R.
name: Mehrabi-Koushki, M.
name: Mešić, A.
name: Miller, A. N.
name: Mironova, P.
name: Moreau, P. - A.
name: Morte, A.
name: Müller, K.
name: Nagy, L. G.
name: Nanu, S.
name: Navarro-Ródenas, A.
name: Nel, W. J.
name: Nguyen, T. H.
name: Nóbrega, T. F.
name: Noordeloos, M. E.
name: Olariaga, I.
name: Overton, B. E.
name: Ozerskaya, S. M.
name: Palani, P.
name: Pancorbo, F.
name: Papp, V.
name: Pawłowska, J.
name: Pham, T. Q.
name: Phosri, C.
name: Popov, E. S.
name: Portugal, A.
name: Pošta, A.
name: Reschke, K.
name: Reul, M.
name: Ricci, G. M.
name: Rodríguez, A.
name: Romanowski, J.
name: Ruchikachorn, N.
name: Saar, I.
name: Safi, A.
name: Sakolrak, B.
name: Salzmann, F.
name: Sandoval-Denis, M.
name: Sangwichein, E.
name: Sanhueza, L.
name: Sato, T.
name: Sastoque, A.
name: Senn-Irlet, B.
name: Shibata, A.
name: Siepe, K.
name: Somrithipol, S.
name: Spetik, M.
name: Sridhar, P.
name: Stchigel, A. M.
name: Stuskova, K.
name: Suwannasai, N.
name: Tan, Y. P.
name: Thangavel, R.
name: Tiago, I.
name: Tiwari, S.
name: Tkalčec, Z.
name: Tomashevskaya, M. A.
name: Tonegawa, C.
name: Tran, H. X.
name: Tran, N. T.
name: Trovão, J.
name: Trubitsyn, V. E.
name: Van Wyk, J.
name: Vieira, W. A. S.
name: Vila, J.
name: Visagie, C. M.
name: Vizzini, A.
name: Volobuev, S. V.
name: Vu, D. T.
name: Wangsawat, N.
name: Yaguchi, T.
name: Ercole, E.
name: Ferreira, B. W.
name: de Souza, A. P.
name: Vieira, B. S.
name: Groenewald, J. Z.
Publisher: name: Zenodo
Year: 2021
Subjects: Biodiversity
Taxonomy
Fungi
Ascomycota
Sordariomycetes
Hypocreales
Nectriaceae
Fusarium
Fusarium chuoi

description: <i>Fusarium chuoi</i> R. Hill, Gaya, D.T. Vu, Sand.-Den. &amp; Crous, <i>sp. nov.</i> <i>Etymology</i>. From <i>chuối</i>, Vietnamese vernacular name for <i>Musa</i> spp., from which the ex-type strain was isolated. Classification — <i>Nectriaceae, Hypocreales, Sordariomycetes.</i> On SNA and CLA, sporulation abundant from aerial conidiophores and sporodochia. <i>Aerial conidiophores</i> erect or prostrate, copiously branching laterally and sympodially, giving rise to macro-, and rarely, microconidia; <i>aerial conidiogenous cells</i> mono- and polyphialidic, subulate to subcylindrical, smooth- and thin-walled, proliferating sympodially, 6.5–40.5 <b>×</b> 2.5–4 µm, with apical flared collarette and periclinal thickening; aerial conidia of two types: <i>microconidia</i> often produced on prostrate conidiophores, rarely on aerial mycelium, aggregating in false heads, ellipsoidal, subcylindrical to slightly falcate, 0 –1-septate, 8–15 <b>×</b> 2–29.5 µm; <i>macroconidia</i> fusiform to falcate, straight to apically dorsiventrally curved, apex curved to pointed, base obtuse to papillate, 1–3-septate, smooth- and thin-walled; 1-septate conidia: (14–)18–27.5(–29.5) <b>×</b> (2.5–)3–4 μm (av. 22.8 <b>×</b> 3.2 μm); 2-septate conidia: 26–28.5 <b>×</b> 3–4 μm (av. 27.4 <b>×</b> 3.6 μm); 3-septate conidia: (28–)31.5–43(–50.5) <b>×</b> 3–4 μm (av. 37.3 <b>×</b> 3.5 μm). <i>Sporodochia</i> saffron, luteous to ochreous coloured (Rayner 1970), formed abundantly on the agar surface and carnation leaves under nuv. <i>Conidiophores in sporodochia</i>, densely and irregularly branched, bearing apical whorls of 2 – 4 monophialides; sporodochial monophialides subcylindrical, 10–26 <b>×</b> 2.5–4.5 µm, smooth- and thin-walled, with a distinct apical collarette. <i>Sporodochial conidia</i> (macroconidia) falcate, almost straight to gently curved, tapering at both ends, apex curved to blunt, base poorly- to well-developed foot-shaped, 1–6-septate, hyaline, smooth- and thin-walled; 1-septate conidia: (14.5–)15–20.5(–24) <b>×</b> 3–4.5 µm (av. 17.9 <b>×</b> 3.9 µm); 2-septate conidia: 21.5–32 <b>×</b> 3–4.5 µm (av. 26.4 <b>×</b> 3.5 µm); 3-septate conidia: (33–)43–61(–71.5) <b>×</b> (3–)4–5 µm (av. 51.8 <b>×</b> 4.2 µm); 4-septate conidia: (50.5–)55–69(–74.5) <b>×</b> 3.5–5 µm (av. 62.3 <b>×</b> 4.2 µm); 5-septate conidia: 54 <b>×</b> 4.5 µm (rare); 6-septate conidia: (49.5–)56.5–71(–73) <b>×</b> (3.5–)4–4.5(–5) µm (av. 63.8 <b>×</b> 4.3 µm). <i>Chlamydospores</i> not observed. Culture characteristics — Colonies on potato dextrose agar (PDA) and oatmeal agar (OA) growing in the dark at 24 ° C covering and entire 9 cm Petri dish in 7 d. Colony surface peach to vinaceous, flat, velvety to felty with abundant floccose aerial mycelium forming concentric rings; colony margins undulate. Reverse flesh to salmon with diffuse coral to brick pigment throughout the medium. <i>Typus</i>. VIETNAM, Hà T&amp;itilde;nh Province, HƯƠng SƠn District,SƠn Kim commune, N18°25'37.38" E105°12'53.95", inside seed of <i>Musa itinerans</i> (<i>Musaceae</i>), 9 Nov. 2014, <i>D.M. Thu, L.T. Phong &amp; T.T. Duong</i>, isol. <i>R. Hill</i> (holotype CBS H-24901,culture ex-type CBS 148464; ITS, LSU, <i>cmdA</i>, <i>rpb1</i>, <i>rpb2</i>, <i>tef1</i> and <i>tub2</i> sequences GenBank OK586454, OK586452, OK626304, OK626306, OK626302, OK626308 and OK626310, MycoBank MB 841865). <i>Colour illustrations</i>. Flowers, fruits, leaves and seeds of <i>Musa itinerans</i> (background photo by D. T. Vu); from top to bottom and left to right: colony on PDA after 14 d at 24 ° C in darkness (left = obverse,right = reverse), sporodochia formed on CLA,aerial conidiophore,aerial conidiogenous cells,aerial conidia, sporodochial conidia. Scale bars: black = 20 µm, white = 10 µm. <i>Additional material examined</i>. VIETNAM, Nghệ An Province, Con Cuông District, Châu Khê commune, N19°1'48.73" E104°43'31.97", inside seed of <i>M. itinerans</i>, 18 Nov. 2014, <i>L.T. Phong, V.V. Tung &amp; T.T. Duong</i>, isol. <i>R. Hill</i> (culture CBS 148465; ITS, LSU, <i>cmdA</i>, <i>rpb1</i>, <i>rpb2</i>, <i>tef1</i> and <i>tub2</i> sequences GenBank OK586455, OK586453, OK626305, OK626307, OK626303, OK626309 and OK626311). Notes — <i>Fusarium chuoi</i> resides in the Asian clade of the <i>Fusarium fujikuroi</i> species complex (FFSC: O’Donnell et al. 1998, Yilmaz et al. 2021, Crous et al. 2021b). Based on nucleotide searches using the <i>Fusarium</i> Pairwise ID engine on the Fusarioid-ID database (www.fusarium.org, Crous et al. 2021) the closest hit using the <b>ITS</b> sequence was <i>Fusarium siculi</i> (strain CBS 142422; identities = 449/450 (99 %), no gaps). The closest hit using the <b>LSU</b> sequence was <i>F. siculi</i> (strain CBS 142422; identities = 804/805 (99 %), no gaps). Closest hit using the <i>cmdA</i> sequence was <i>Fusarium fractiflexum</i> (strain NRRL 28852; identities = 426/434 (98 %), no gaps). Closest hit using the <i>rpb1</i> sequence was <i>F. fujikuroi</i> (strain NRRL 13566; identities = 687/702 (98 %), no gaps). Closest hit using the <i>rpb2</i> sequence was <i>Fusarium globosum</i> (strain CBS 428.97; identities = 856/867 (98 %), no gaps). Closest hit using the <i>tef1</i> sequence was <i>F. fractiflexum</i> (strain NRRL 28852; identities = 619/643 (96 %), 2 gaps (0.3 %)). The phylogenetic results, however, showed that <i>F. chuoi</i> is not directly related to any of the previously described species of FFSC (see Suppl. material FP1353), clustering as the second basal-most species of that clade after <i>F. sacchari</i>. Asian <i>Fusarium</i> spp. in the FFSC are characterised by mono-and polyphialides producing oval to ellipsoid, rarely pyriform to globose (i.e., <i>F. annulatum</i>, <i>F. fujikuroi</i> and <i>F. globosum</i>) microconidia organized in chains or false heads; 3–5-septate sporodochial conidia and lacking chlamydospores. The elaborate, profusely branched aerial conidiophores of <i>F. chuoi</i> are comparable to those of <i>F. concentricum</i>, <i>F. lumajangense</i>, <i>F. mangiferae</i> and <i>F. sacchari</i>, all the latter species producing oval, ellipsoidal to allantoid microconidia on false heads.Aerial conidiophores of <i>F. chuoi</i>, however, mostly produce macroconidia, while microconidia grouped on false heads are restricted to short, mostly unbranched and prostrate conidiophores formed on the surface on the culture media. Several Asian species of the FFSC have been reported from <i>Musa</i> spp. i.e., <i>F. annulatum</i>, <i>F. concentricum</i>, <i>F. fujikuroi</i>, <i>F. lumajangense</i> and <i>F. sacchari</i> (Leslie &amp; Summerell 2006, Maryani et al. 2019, Farr &amp; Rossman 2021). The two strains representing <i>F. chuoi</i> were isolated as endophytes from asymptomatic seeds of wild banana (<i>Musa itinerans</i>), which had been collected predispersal and stored in the Millennium Seed Bank for ~2.5 years at -20 ° C prior to isolation. Supplementary material <b>FP1353</b> Phylogenetic tree.
description: Published as part of <i>Crous, P. W., Osieck, E. R., Jurjevi, Ž, Boers, J., Van Iperen, A. L., Starink-Willemse, M., Dima, B., Balashov, S., Bulgakov, T. S., Johnston, P. R., Morozova, O. V., Pinruan, U., Sommai, S., Alvarado, P., Decock, C. A., Lebel, T., McMullan-Fisher, S., Moreno, G., Shivas, R. G., Zhao, L., Abdollahzadeh, J., Abrinbana, M., Ageev, D. V., Akhmetova, G., Alexandrova, A. V., Altés, A., Amaral, A. G. G., Angelini, C., Antonín, V., Arenas, F., Asselman, P., Badali, F., Baghela, A., Bañares, A., Barreto, R. W., Baseia, I. G., Bellanger, J. - M., Berraf-Tebbal, A., Biketova, A. Yu., Bukharova, N. V., Burgess, T. I., Cabero, J., Câmara, M. P. S., Cano-Lira, J. F., Ceryngier, P., Chávez, R., Cowan, D. A., de Lima, A. F., Oliveira, R. L., Denman, S., Dang, Q. N., Dovana, F., Duarte, I. G., Eichmeier, A., Erhard, A., Esteve-Raventós, F., Fellin, A., Ferisin, G., Ferreira, R. J., Ferrer, A., Finy, P., Gaya, E., Geering, A. D. W., Gil-Durán, C., Glässnerová, K., Glushakova, A. M., Gramaje, D., Guard, F. E., Guarnizo, A. L., Haelewaters, D., Halling, R. E., Hill, R., Hirooka, Y., Hubka, V., Iliushin, V. A., Ivanova, D. D., Ivanushkina, N. E., Jangsantear, P., Justo, A., Kachalkin, A. V., Kato, S., Khamsuntorn, P., Kirtsideli, I. Y., Knapp, D. G., Kochkina, G. A., Koukol, O., Kovács, G. M., Kruse, J., Kumar, T. K. A., Kušan, I., Læssøe, T., Larsson, E., Lebeuf, R., Levicán, G., Loizides, M., Marinho, P., Luangsa-ard, J. J., Lukina, E. G., Magaña-Dueñas, V., Maggs-Kölling, G., Malysheva, E. F., Malysheva, V. F., Martín, B., Martín, M. P., Matočec, N., McTaggart, A. R., Mehrabi-Koushki, M., Mešić, A., Miller, A. N., Mironova, P., Moreau, P. - A., Morte, A., Müller, K., Nagy, L. G., Nanu, S., Navarro-Ródenas, A., Nel, W. J., Nguyen, T. H., Nóbrega, T. F., Noordeloos, M. E., Olariaga, I., Overton, B. E., Ozerskaya, S. M., Palani, P., Pancorbo, F., Papp, V., Pawłowska, J., Pham, T. Q., Phosri, C., Popov, E. S., Portugal, A., Pošta, A., Reschke, K., Reul, M., Ricci, G. M., Rodríguez, A., Romanowski, J., Ruchikachorn, N., Saar, I., Safi, A., Sakolrak, B., Salzmann, F., Sandoval-Denis, M., Sangwichein, E., Sanhueza, L., Sato, T., Sastoque, A., Senn-Irlet, B., Shibata, A., Siepe, K., Somrithipol, S., Spetik, M., Sridhar, P., Stchigel, A. M., Stuskova, K., Suwannasai, N., Tan, Y. P., Thangavel, R., Tiago, I., Tiwari, S., Tkalčec, Z., Tomashevskaya, M. A., Tonegawa, C., Tran, H. X., Tran, N. T., Trovão, J., Trubitsyn, V. E., Van Wyk, J., Vieira, W. A. S., Vila, J., Visagie, C. M., Vizzini, A., Volobuev, S. V., Vu, D. T., Wangsawat, N., Yaguchi, T., Ercole, E., Ferreira, B. W., de Souza, A. P., Vieira, B. S. &amp; Groenewald, J. Z., 2021, Fusarium chuoi R. Hill, Gaya, D. T. Vu, Sand. - Den. &amp; Crous, R. Hill, Gaya, D. T. Vu, Sand. - Den. &amp; Crous sp. nov., pp. 310-311 in Fungal Planet 47 (1)</i> on page 311, DOI: 10.5281/zenodo.5856199
description: {"references": ["Rayner RW. 1970. A Mycological Colour Chart. Commonwealth Mycological Institute, Kew and British Mycological Society.", "O'Donnell K, Cigelnik E, Nirenberg H. 1998. Molecular systematics and phylogeography of the Gibberella fujikuroi species complex. Mycologia 90: 465 - 493.", "Yilmaz N, Sandoval-Denis M, Lombard L, et al. 2021. Redefining species limits in the Fusarium fujikuroi species complex. Persoonia 46: 129 - 162.", "Crous PW, Lombard L, Sandoval-Denis M, et al. 2021 b. Fusarium: more than a node or a foot-shaped basal cell. Studies in Mycology 98: 100116.", "Crous PW, Cowan DA, Maggs-Kolling G, et al. 2021 a. Fungal Planet descrip- tion sheets: 1182 - 1283. Persoonia 46: 313 - 528.", "Leslie JF, Summerell BA. 2006. The Fusarium laboratory manual. Blackwell Publishing, Ames.", "Maryani N, Sandoval-Denis M, Lombard L, et al. 2019. New endemic Fusarium species hitch-hiking with pathogenic Fusarium strains causing Panama disease in small-holder banana plots in Indonesia. Persoonia 43: 48 - 69."]}