Additional file 1 of Validating Star-Oddi heart rate and acceleration data storage tags for use in Atlantic salmon (Salmo salar)
Creators: name: Zrini, Zoe Amanda
name: A. Kurt Gamperl
Publisher: name: figshare
Year: 2021
Subjects: subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: FOS: Earth and related environmental sciences
subject: Ecology
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Inorganic Chemistry
subject: FOS: Chemical sciences
subject: FOS: Chemical sciences
subject: Science Policy
subject: 111714 Mental Health
subject: FOS: Health sciences
subject: FOS: Health sciences

description: Additional file 1: Figure S1. Milli-TD DSTs (that record depth and temperature) were attached to the fish externally using a “plate holder kit” provided by Star-Oddi and stainless steel wire (0.02″ diameter). Tags were prepared for attachment by looping pre-sterilized stainless steel wire over the tag and passing the ends of the wire through one of the kit’s silicone pads and the pre-drilled holes in the kit’s plastic mold. Four pre-sterilized stainless steel hypodermic needles (15 gauge, 3.5″ long) were then passed through the skin and muscle below the dorsal fin to allow the stainless steel wire to be guided through. Then, the hypodermic needles were removed and the 4 wires exiting the muscle were passed through the kit’s other silicone pad and plastic mold, and the wires were twisted together to secure the DST to the fish. Figure S2. An electrocardiogram (ECG) recorded in a salmon during 1 week of recovery. The ECG was randomly chosen to represent the typical recording from a salmon, where Bin ECG represents the amplitude of the PQRS waveform and ranges from 0 to 1000 mV. Heart rate (fH) was calculated from the ECGs as the time between R wave peaks (measured in seconds). These values were then averaged, and 60 was divided by the average to obtain the fish’s fH in bpm. Heart rate variability (HRV) was calculated as the standard deviation of the time between successive R wave peaks (in ms). Table S1. Photographs of the Star-Oddi data storage tags used in this paper. Further information on these products can be found at https://www.star-oddi.com . Table S2. Linear mixed-effects models were used to determine the relationships between external acceleration (EA, mg) and swimming speed (BL s−1) and tail beat frequency (tail beats min−1), and between the latter two parameters and variation in EA (VAR, mg2), with fish as a random factor. The lowest Akaike information criterion (AIC) value was used to select between the two models (linear regression and log transformed data). Table S3. Summary of the statistical outputs from linear mixed-effects models that were used to examine the effects of swimming speed on heart rate parameters in Atlantic salmon. Linear mixed-effects models were used to assess the effects of swimming speed (body lengths s−1) on heart rate, heart rate variability and the percentage of quality index values equal to zero (QI0). Significant differences are shown in bold font. Table S4. Summary of the statistical outputs from the linear mixed-effects models that examined the effects of night/day (photoperiod), days post-implantation, and their interaction, on fH parameters [fH and percentage of quality index values equal to zero (QI0)] and external acceleration (EA) in salmon for 7 days post-surgery. Significant differences are shown in bold. Table S5. Summary of the statistical output from the linear mixed-effects models that examined the effects of night/day (photoperiod), at every 10 days or for all days post-implantation, on fH parameters [fH and percentage of quality index values equal to zero (QI0)] and external acceleration (EA) in Atlantic salmon for 6 weeks post-implantation, and their interaction. Due to issues with facility temperature control, the data is separated into two trials with a tank temperature of 8 °C in trial 1 (a 10 days; b all data) and 8–12 °C in trial 2 (c).