title: ÐÐ´ÐµÐ½ÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ñ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸ Ð°ÐºÑÐ¸Ð²Ð½ÑÑ Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸ÑÐ¾Ð² Ð¸ ÑÑÐ¸Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð² Ð² ÐºÑÐ»ÑÑÑÑÐ°Ð»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ´Ðµ ÑÑÐ°Ð¼Ð¼Ð¾Ð² L.helveticus D75 Ð¸ D76
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Identification of biologically active metabolites and tripeptides in the culture medium of strains of L.helveticus D75 and D76
Creators: name: ÐÑÐ²Ð°ÐºÐ¸Ð½, ÐÐ¸ÑÐ¸Ð»Ð»
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: ÐÐ¸Ð´ÑÐ¾Ð»Ð¸Ð·
ÐÐµÐ¿ÑÐ¸Ð´Ñ
Î²-ÐºÐ°Ð·ÐµÐ¸Ð½
lactobacillus helveticus d75 Ð¸ d76
Ð°Ð½ÑÐ¸Ð³Ð¸Ð¿ÐµÑÑÐµÐ½Ð·Ð¸Ð²Ð½Ð¾Ðµ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ðµ
Ð²Ð°Ð»Ð¸Ð½-Ð¿ÑÐ¾Ð»Ð¸Ð½-Ð¿ÑÐ¾Ð»Ð¸Ð½
Ð¸Ð·Ð¾Ð»ÐµÐ¹ÑÐ¸Ð½-Ð¿ÑÐ¾Ð»Ð¸Ð½-Ð¿ÑÐ¾Ð»Ð¸Ð½
Ð¼Ð¾Ð»Ð¾ÐºÐ¾
Ð¿ÑÐ¾Ð±Ð¸Ð¾ÑÐ¸ÐºÐ¸
Î²-casein
antihypertensive effect
hydrolysis
ile-pro-pro
lactobacillus helveticus d75 and d76
milk
probiotics
val-pro-pro

description: ÐÐ° 66 Ñ., 6 ÑÐ°Ð±Ð»., 30 ÑÐ¸Ñ. Ð¦ÐµÐ»ÑÑ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÐ»ÑÑ Ð¿Ð¾Ð¸ÑÐº Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸ Ð°ÐºÑÐ¸Ð²Ð½ÑÑ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð² Ð¸Ð·Ð¾Ð»ÐµÐ¹ÑÐ¸Ð½-Ð¿ÑÐ¾Ð»Ð¸Ð½-Ð¿ÑÐ¾Ð»Ð¸Ð½ (ÐÐÐ, Ile-Pro-Pro) Ð¸ Ð²Ð°Ð»Ð¸Ð½-Ð¿ÑÐ¾Ð»Ð¸Ð½-Ð¿ÑÐ¾Ð»Ð¸Ð½ (ÐÐÐ, Val-Pro-Pro) Ñ Ð°Ð½ÑÐ¸Ð³Ð¸Ð¿ÐµÑÑÐµÐ½Ð·Ð¸Ð²Ð½ÑÐ¼ Ð´ÐµÐ¹ÑÑÐ²Ð¸ÐµÐ¼, Ð° ÑÐ°ÐºÐ¶Ðµ ÐºÐ°ÑÐ±Ð¾Ð½Ð¾Ð²ÑÑ Ð¸ Ð°Ð¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾Ñ Ð² ÐºÑÐ»ÑÑÑÑÐ°Ð»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ´Ðµ (ÐÐ¡) Ð¿ÑÐ¾Ð±Ð¸Ð¾ÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÑÐ°Ð¼Ð¼Ð¾Ð² Lactobacillus helveticus D75 Ð¸ D76 Ð¿ÑÐ¸ Ð¸Ñ Ð²ÑÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ð¸ Ð½Ð° Ð¼Ð¾Ð»Ð¾ÐºÐµ. ÐÐ½Ð°Ð»Ð¸Ð· ÑÐ¾ÑÑÐ°Ð²Ð° ÐÐ¡ Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»Ð¸ Ð¼ÐµÑÐ¾Ð´Ð°Ð¼Ð¸ ÐÐ­ÐÐ¥-Ð£Ð¤, Ð£ÐÐ­ÐÐ¥-ÐÐ¡ Ð¸ ÐÐÐÐÐ-Ð¼Ð°ÑÑ-ÑÐ¿ÐµÐºÑÑÐ¾Ð¼ÐµÑÑÐ¸Ð¸ Ð¸Ð¾Ð½Ð½Ð¾-ÑÐ¸ÐºÐ»Ð¾ÑÑÐ¾Ð½Ð½Ð¾Ð³Ð¾ ÑÐµÐ·Ð¾Ð½Ð°Ð½ÑÐ° (MALDI FT-ICR MS, Varian). ÐÑÐ¸ Ð½ÐµÐ¿Ð¾ÑÑÐµÐ´ÑÑÐ²ÐµÐ½Ð½Ð¾Ð¼ Ð°Ð½Ð°Ð»Ð¸Ð·Ðµ ÐÐ¡ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ FT-ICR Ð¾Ð±Ð½Ð°ÑÑÐ¶Ð¸ÑÑ Ð¿ÐµÐ¿ÑÐ¸Ð´Ñ Ð² ÑÐ¾ÑÑÐ°Ð²Ðµ Ð¸ÑÑÐ¾Ð´Ð½ÑÑ ÐÐ¡ Ð½Ðµ ÑÐ´Ð°Ð»Ð¾ÑÑ Ð¸Ð·-Ð·Ð° Ð²ÑÑÐ¾ÐºÐ¾Ð¹ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ ÑÐ¾Ð¿ÑÑÑÑÐ²ÑÑÑÐ¸Ñ ÐºÐ¾Ð¼Ð¿Ð¾Ð½ÐµÐ½ÑÐ¾Ð², Ð¾Ð´Ð½Ð°ÐºÐ¾ Ð¿Ð¾ÑÐ»Ðµ Ð¿ÑÐµÐ´Ð²Ð°ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ ÑÑÐ°ÐºÑÐ¸Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÐÐ¡ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ ÐÐ­ÐÐ¥ Ð¾Ð±Ð° Ð¿ÐµÐ¿ÑÐ¸Ð´Ð° Ð±ÑÐ»Ð¸ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ñ. ÐÐ°Ð¹Ð´ÐµÐ½Ð½ÑÐµ Ð¼Ð°ÑÑÑ (m/z) ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð¸Ð¾Ð½Ð¾Ð² ÑÐ¾ÑÑÐ°Ð²Ð»ÑÐ»Ð¸ 312,19 (ÐÐÐ) Ð¸ 326,21 (ÐÐÐ). ÐÐ¾Ð»Ð¸ÑÐµÑÑÐ²ÐµÐ½Ð½Ð¾Ðµ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð² Ð±ÑÐ»Ð¾ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¾ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð¾Ð´Ð½Ð¾Ð³Ð¾ ÑÑÐ°Ð»Ð¾Ð½Ð° Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ ÐÐ­ÐÐ¥ Ð¸ Ð²ÑÐµÐ¼ÑÐ¿ÑÐ¾Ð»ÐµÑÐ½Ð¾Ð¹ Ð¼Ð°ÑÑ-ÑÐ¿ÐµÐºÑÑÐ¾Ð¼ÐµÑÑÐ¸Ð¸. ÐÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ ÐÐÐ Ð¸ ÐÐÐ, ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²ÐµÐ½Ð½Ð¾, Ð² ÐÐ¡ ÑÑÐ°Ð¼Ð¼Ð° D75 Ð´Ð¾ÑÑÐ¸Ð³Ð°Ð»Ð¸ 18,0 Ð¼ÐºÐ³/Ð¼Ð» Ð¸ 25,4 Ð¼ÐºÐ³/Ð¼Ð», Ð² ÐÐ¡ ÑÑÐ°Ð¼Ð¼Ð° D76 Ð¾Ð½Ð¸ Ð±ÑÐ»Ð¸ Ð¼ÐµÐ½ÑÑÐµ â 12,7 Ð¼ÐºÐ³/Ð¼Ð» Ð¸ 16,2 Ð¼ÐºÐ³/Ð¼Ð», Ð° Ð¿ÑÐ¸ Ð²ÑÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ð¸ ÑÐ¼ÐµÑÐ°Ð½Ð½Ð¾Ð¹ ÐºÑÐ»ÑÑÑÑÑ Ð¾Ð±Ð¾Ð¸Ñ ÑÑÐ°Ð¼Ð¼Ð¾Ð² Ð´Ð¾ÑÑÐ¸Ð³Ð°Ð»Ð¸ Ð½ÐµÐºÐ¾ÑÐ¾ÑÐ¾Ð³Ð¾ Ð¿ÑÐ¾Ð¼ÐµÐ¶ÑÑÐ¾ÑÐ½Ð¾Ð³Ð¾ Ð·Ð½Ð°ÑÐµÐ½Ð¸Ñ â 15,7 Ð¼ÐºÐ³/Ð¼Ð» Ð¸ 24,0 Ð¼ÐºÐ³/Ð¼Ð». ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ Ð´Ð°Ð½Ð½ÑÐµ ÑÐ²Ð¸Ð´ÐµÑÐµÐ»ÑÑÑÐ²ÑÑÑ Ð¾ ÑÑÑÐµÑÑÐ²Ð¾Ð²Ð°Ð½Ð¸Ð¸ Ð°ÐºÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ° Ð²Ð½ÐµÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ ÑÐµÑÐ¼ÐµÐ½ÑÐ¾Ð² Ñ L. helveticus D75 Ð¸ D76, Ð¾Ð±ÐµÑÐ¿ÐµÑÐ¸Ð²Ð°ÑÑÐ¸Ñ Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð² ÐÐÐ Ð¸ ÐÐÐ Ñ Ð°Ð½ÑÐ¸Ð³Ð¸Ð¿ÐµÑÑÐµÐ½Ð·Ð¸Ð²Ð½ÑÐ¼ Ð´ÐµÐ¹ÑÑÐ²Ð¸ÐµÐ¼, Ð¸ Ð¿Ð¾Ð´ÑÐ²ÐµÑÐ¶Ð´Ð°ÑÑ Ð½Ð°Ð»Ð¸ÑÐ¸Ðµ Ñ Ð½Ð¸Ñ ÑÐ¸Ð¼Ð±Ð¸Ð¾ÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÐ²Ð¾Ð¹ÑÑÐ² ÑÐ¸Ð½ÐµÑÐ³Ð¸Ð·Ð¼Ð° Ð¸ ÑÐ¸Ð½ÑÑÐ¾ÑÐ¸Ð¸. Ð Ð°Ð±Ð¾ÑÐ° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð² Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ Ð¿ÑÐ¸ÐºÐ»Ð°Ð´Ð½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¤ÐÐ£Ð Â«ÐÐ¾Ñ. ÐÐÐ ÐÐ§ÐÂ» Ð¤ÐÐÐ Ð Ð¾ÑÑÐ¸Ð¸ Ð¿Ð¾Ð´ ÑÑÐºÐ¾Ð²Ð¾Ð´ÑÑÐ²Ð¾Ð¼ Ð½Ð°ÑÑÐ½Ð¾Ð³Ð¾ ÐºÐ¾Ð½ÑÑÐ»ÑÑÐ°Ð½ÑÐ° ÐÐ°ÑÐ¸ÑÐ¾Ð²Ð° Ð¢. Ð¯.
description: 66 pages, 6 tables, 30 pictures The aim of the work was to search for bioactive peptides Ile-Pro-Pro (IPP) and Val-Pro-Pro (VPP) with antihypertensive action, as well as carboxylic and amino acids in the culture medium (CM) of probiotic Lactobacillus helveticus D75 and D76 strains when cultured in milk. The CM composition was analyzed using HPLC-UV, HPLC-MS and MALDI Fourier transformâion cyclotron resonance mass spectrometry (MALDI FT-ICR MS, Varian). Direct analysis of CM using FT-ICR failed to detect peptides in the original CM due to the high concentration of concomitant components, but after preliminary fractionation of CM by HPLC, both peptides were detected. The masses (m/z) of characteristic ions were 312.19 (VPP) and 326.21 (IPP). Peptides were quantified using a single reference method with HPLC and time-of-flight mass spectrometry. The concentrations of VPP and PPI in the CM of D75 strain reached 18.0 Âµg/ml and 25.4 Âµg/ml, in the CM of D76 strain they were lower (12.7 Âµg/ml and 16.2 Âµg/ml), and in the mixed CM (both strains) the concentrations reached intermediate values of 15.7 Âµg/ml and 24.0 Âµg/ml. The data obtained indicate the existence of an active complex of extracellular enzymes in L. helveticus D75 and D76, which provide the production of IPP and VPP peptides with antihypertensive effect. The results also confirm the symbiotic properties of D75 and D76 strains (synergism and syntrophy). The work was carried out on the basis of the laboratory of applied microbiology of the State Research Institute of Highly Pure Biopreparation FMBA of Russia under the guidance of scientific adviser Vakhitov T.Y.