Additional file 1 of Non-targeted discovery of high-value bio-products in Nicotiana glauca L: a potential renewable plant feedstock
Creators: name: Carreno-Quintero, Natalia
name: Tohge, Takayuki
name: Van Acker, Rebecca
name: McKee, Lauren S.
name: Zhou, Qi
name: Bolze, Antje
name: Xing, Xiaohui
name: Özparpucu, Merve
name: Rüggeberg, Markus
name: Piofczyk, Thomas
name: Koram, Yaw
name: Bulone, Vincent
name: Boerjan, Wout
name: Fernie, Alisdair R.
name: Fraser, Paul D.
Publisher: name: figshare
Year: 2024
Subjects: subject: Biochemistry
subject: Cell Biology
subject: Molecular Biology
subject: Pharmacology
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: FOS: Biological sciences
subject: Biological Sciences not elsewhere classified
subject: Inorganic Chemistry
subject: FOS: Chemical sciences
subject: Hematology
subject: Plant Biology

description: Additional file 1: Figure S1. Schematic diagram of extraction procedures used to enrich metabolites for tobacco tree biomass. Figure S2. Relative metabolite content in the wax fraction of mature leaves N. glauca. Figure S3. Effect of a pre-treatment after 72 h of saccharification, including glucose release per dry weight, glucose release per cell wall residue (CWR) and cellulose conversion for N. glauca, maize and poplar. Bars represent averages of 5 biological replicates, error bars are standard deviations. * indicates significant changes compared to maize and † indicates significant changes compared to poplar within each pre-treatment (T test, P &lt; 0.05). Table S1. Relative abundance of non-polar metabolites detected in non-polar extract 1 and 2 of mature leaves of N. glauca by GC–MS analysis. Metabolites were identified partly following the metabolomics reporting standards [1]. Relative abundance was evaluated by normalization. Table S2. Polar metabolites detected in aqueous fraction of mature leaves of N. glauca by GC–MS analysis. Metabolites were identified in comparison to database entries of authentic standards (Kopka et al 2005; Schauer et al 2005). Relative abundance was evaluated by normalization. Table S3. Glucose releases, expressed as percentage dry weight (DW) or percentage cell wall residue (CWR), after 72 h of saccharification. Based on the measured crystalline cellulose contents, the cellulose conversion could be calculated. Values are the means of 5 biological replicates ± SD. Values in bold correspond to the highest value in each treatment. T test comparisons between N. glauca and maize (*) and N. glauca and poplar (†) represent statistical significance at the 0.05 threshold. Table S4. Cell wall composition and cellulose crystallinity in N. glauca tissues. Composition values are presented as µg of component per mg of dry cell wall, where cell wall material does not include ash, proteins or other extractives. Values are the means for six biological replicate samples, each analysed in duplicate ± SD. Cellulose crystallinity index (CrI) values were calculated from XRD data shown in Fig. S4. Fuc: Fucose, Ara: Arabinose, Rha: Rhamnose, Gal: Galactose, Glc: Glucose, Xyl: Xylose. Table S5. Average values and standard deviation of mechanical Properties of xylem of mature stems of N. glauca extracted from micro-tensile tests of tissue slices (n = 33).