title: Ð ÐµÐ³ÑÐ»ÑÑÐ¸Ñ Ð´ÐµÐ¿Ð¾-ÑÐ¿ÑÐ°Ð²Ð»ÑÐµÐ¼ÑÐ¼ Ð²Ñ Ð¾Ð´Ð¾Ð¼ ÐºÐ°Ð»ÑÑÐ¸Ñ ÑÐ½Ð´Ð¾Ð³ÐµÐ½Ð½ÑÑ ÐºÐ°Ð»ÑÑÐ¸Ð¹-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼ÑÑ Ñ Ð»Ð¾ÑÐ½ÑÑ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² ANO6 Ð² ÐºÐ»ÐµÑÐºÐ°Ñ HEK293
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Regulation of endogenous calcium activated chloride channels ANO6 in HEK293 cells by store-operated calcium entry
Creators: name: ÐÑÐ¸Ð³Ð¾ÑÑÐµÐ²Ð°, ÐÐ²Ð³ÐµÐ½Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: ÐºÐ°Ð»ÑÑÐ¸Ð¹-Ð°ÐºÑÐ¸Ð²Ð¸ÑÑÐµÐ¼ÑÐµ Ñ Ð»Ð¾ÑÐ½ÑÐµ ÐºÐ°Ð½Ð°Ð»Ñ
Ð´ÐµÐ¿Ð¾-ÑÐ¿ÑÐ°Ð²Ð»ÑÐµÐ¼ÑÐ¹ Ð²Ñ Ð¾Ð´ ÐºÐ°Ð»ÑÑÐ¸Ñ
ÑÐ°Ð¿ÑÐ¸Ð³Ð°ÑÐ³Ð¸Ð½
Ð½Ð¸ÑÐ»ÑÐ¼Ð¾Ð²Ð°Ñ ÐºÐ¸ÑÐ»Ð¾ÑÐ°
calcium-activated chloride channels
store-operated calcium entry
thapsigargin
niflumic acid

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ð¼ÐµÐ¶Ð´Ñ Ð´ÐµÐ¿Ð¾-ÑÐ¿ÑÐ°Ð²Ð»ÑÐµÐ¼ÑÐ¼Ð¸ ÐºÐ°Ð½Ð°Ð»Ð°Ð¼Ð¸ Orai Ð¸ ÑÐ½Ð´Ð¾Ð³ÐµÐ½Ð½ÑÐ¼Ð¸ ÐºÐ°Ð»ÑÑÐ¸Ð¹-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼ÑÐ¼Ð¸ ÑÐ»Ð¾ÑÐ½ÑÐ¼Ð¸ ÐºÐ°Ð½Ð°Ð»Ð°Ð¼Ð¸ (Ð¡Ð°Ð¡Ð¡) ANO6 Ð² ÐºÐ»ÐµÑÐºÐ°Ñ HEK293. ÐÐ½Ð¾ÐºÑÐ°Ð¼Ð¸Ð½ 6 (ANO6/TMEM16F) ÑÐ¾Ð²Ð¼ÐµÑÐ°ÐµÑ Ð² ÑÐµÐ±Ðµ ÑÑÐ½ÐºÑÐ¸Ð¸ ÐºÐ°Ð»ÑÑÐ¸Ð¹-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾Ð³Ð¾ Ð¸Ð¾Ð½Ð½Ð¾Ð³Ð¾ ÐºÐ°Ð½Ð°Ð»Ð° Ð¸ ÑÐ¾ÑÑÐ¾Ð»Ð¸Ð¿Ð¸Ð´Ð½Ð¾Ð¹ ÑÐºÑÐ°Ð¼Ð±Ð»Ð°Ð·Ñ. ÐÐ°ÑÑÑÐµÐ½Ð¸Ðµ ÐµÐ³Ð¾ ÑÐ°Ð±Ð¾ÑÑ Ð¿ÑÐ¸Ð²Ð¾Ð´Ð¸Ñ Ðº ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ñ Ð¼Ð½Ð¾Ð¶ÐµÑÑÐ²Ð° Ð¿Ð°ÑÐ¾Ð»Ð¾Ð³Ð¸Ð¹ Ð¿Ð¾ÑÑÐ¸ Ð²Ð¾ Ð²ÑÐµÑ Ð¾ÑÐ³Ð°Ð½Ð°Ñ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°. ÐÐ»Ñ Ð°ÐºÑÐ¸Ð²Ð°ÑÐ¸Ð¸ ANO6 ÑÑÐµÐ±ÑÑÑÑÑ Ð²ÑÑÐ¾ÐºÐ°Ñ Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½Ð°Ñ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ñ ÐºÐ°Ð»ÑÑÐ¸Ñ. ÐÐµÐ¿Ð¾-ÑÐ¿ÑÐ°Ð²Ð»ÑÐµÐ¼ÑÐ¹ Ð²ÑÐ¾Ð´ ÐºÐ°Ð»ÑÑÐ¸Ñ, Ð¾Ð¿Ð¾ÑÑÐµÐ´Ð¾Ð²Ð°Ð½Ð½ÑÐ¹ ÐºÐ°Ð»ÑÑÐ¸ÐµÐ²ÑÐ¼Ð¸ ÑÐµÐ½ÑÐ¾ÑÐ°Ð¼Ð¸ STIM Ð¸ Ð´ÐµÐ¿Ð¾-ÑÐ¿ÑÐ°Ð²Ð»ÑÐµÐ¼ÑÐ¼Ð¸ ÐºÐ°Ð½Ð°Ð»Ð°Ð¼Ð¸ Orai ÑÐ²Ð»ÑÐµÑÑÑ Ð¾Ð´Ð½Ð¸Ð¼ Ð¸Ð· Ð¾ÑÐ½Ð¾Ð²Ð½ÑÑ Ð¸ÑÑÐ¾ÑÐ½Ð¸ÐºÐ¾Ð² ÐºÐ°Ð»ÑÑÐ¸Ñ Ð² ÑÐ»ÐµÐºÑÑÐ¾-Ð½ÐµÐ²Ð¾Ð·Ð±ÑÐ´Ð¸Ð¼ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ. ÐÑÐ¸ Ð¿Ð¾Ð¼Ð¾ÑÐ¸ Ð¼ÐµÑÐ¾Ð´Ð° Ð»Ð¾ÐºÐ°Ð»ÑÐ½Ð¾Ð¹ ÑÐ¸ÐºÑÐ°ÑÐ¸Ð¸ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð°, ÑÐµÐ³Ð¸ÑÑÑÐ¸ÑÑÑ Ð¸Ð¾Ð½Ð½ÑÐµ ÑÐ¾ÐºÐ¸ ÑÐµÑÐµÐ· Ð¾Ð´Ð¸Ð½Ð¾ÑÐ½ÑÐµ ÐºÐ°Ð½Ð°Ð»Ñ, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¸Ð½ÑÐµÐ³ÑÐ°Ð»ÑÐ½ÑÐ¹ ÑÐ¾Ðº ÑÐµÑÐµÐ· Ð¿Ð»Ð°Ð·Ð¼Ð°ÑÐ¸ÑÐµÑÐºÑÑ Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ñ Ð² ÐºÐ»ÐµÑÐºÐ°Ñ HEK293, Ð¼Ñ Ð²Ð¿ÐµÑÐ²ÑÐµ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð»Ð¸, ÑÑÐ¾ ÑÐ½Ð´Ð¾Ð³ÐµÐ½Ð½ÑÐµ Ð¡Ð°Ð¡Ð¡ Ð² ÐºÐ»ÐµÑÐºÐ°Ñ HEK293 ÑÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ñ Ð±ÐµÐ»ÐºÐ°Ð¼Ð¸ ANO6. ÐÐ°Ð¼Ð¸ Ð±ÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð¿Ð°ÑÑÐ¸Ð²Ð½Ð¾Ðµ Ð¾Ð¿ÑÑÑÐ¾ÑÐµÐ½Ð¸Ðµ ÐºÐ°Ð»ÑÑÐ¸ÐµÐ²Ð¾Ð³Ð¾ Ð´ÐµÐ¿Ð¾ Ð¿ÑÐ¸Ð²Ð¾Ð´Ð¸Ñ Ðº Ð°ÐºÑÐ¸Ð²Ð°ÑÐ¸Ð¸ ÑÐ½Ð´Ð¾Ð³ÐµÐ½Ð½ÑÑ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² ANO6 Ð² ÐºÐ»ÐµÑÐºÐ°Ñ HEK293. ÐÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÑÐ½Ð´Ð¾Ð³ÐµÐ½Ð½ÑÑ Ð´ÐµÐ¿Ð¾-ÑÐ¿ÑÐ°Ð²Ð»ÑÐµÐ¼ÑÑ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² Orai Ð¿Ð¾Ð´Ð°Ð²Ð»ÑÐ»Ð°ÑÑ ÑÐµÐ»ÐµÐºÑÐ¸Ð²Ð½ÑÐ¼ Ð±Ð»Ð¾ÐºÐ°ÑÐ¾ÑÐ¾Ð¼ Synta66 Ð¸Ð»Ð¸ Ð´Ð¾Ð¼Ð¸Ð½Ð°Ð½ÑÐ½Ð¾-Ð½ÐµÐ³Ð°ÑÐ¸Ð²Ð½ÑÐ¼ Ð¼ÑÑÐ°Ð½ÑÐ¾Ð¼ Orai1 E106Q. Ð¢Ð°ÐºÐ¸Ð¼ Ð¾Ð±ÑÐ°Ð·Ð¾Ð¼, Ð½Ð°ÑÐ¸ Ð´Ð°Ð½Ð½ÑÐµ Ð¿Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÑÑ, ÑÑÐ¾ Ð²ÑÐ¾Ð´ Ð¸Ð¾Ð½Ð¾Ð² ÐºÐ°Ð»ÑÑÐ¸Ñ ÑÐµÑÐµÐ· ÐºÐ°Ð½Ð°Ð» Orai Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼ Ð´Ð»Ñ Ð°ÐºÑÐ¸Ð²Ð°ÑÐ¸Ð¸ ÑÐ½Ð´Ð¾Ð³ÐµÐ½Ð½ÑÑ ANO6 Ð² ÐºÐ»ÐµÑÐºÐ°Ñ HEK293.
description: This work is dedicated to the study of calcium activated chloride channels (CaCC) ANO6 and endogenous store-operated Orai channels. Anoctamine 6 (ANO6/TMEM16F) combines the functions of a calcium-activated ion channel and phospholipid scramblase.Â  Its malfunction leads to the development of many pathologies in almost all human body organs. High intracellular calcium concentrations are required to activate ANO6. Store-operated calcium entry, mediated by STIM calcium sensors and store-operated Orai channels, is one of the main sources of calcium in non-excitable cells. Using patch clamp methods, we have registered currents through single ion channels, as well as a whole cell current across the plasma membrane in HEK293 cells. For the first time we have shown that endogenous CaCC in HEK293 cells are formed by ANO6 proteins. We have shown that passive depletion of the calcium store leads to activation of endogenous ANO6 channels in HEK293 cells. The activity of endogenous store-operated Orai channels was suppressed by the selective Orai inhibitor Synta66 and by the dominant-negative mutant Orai1 E106Q overexpression. Thus, our data show that the entry of calcium ions through the Orai channels is necessary for the activation of endogenous ANO6 channels in HEK293 cells.