title: ÐÐ½Ð°Ð»Ð¸Ð· ÐºÐ»Ð°ÑÑÐµÑÐ¸Ð·Ð°ÑÐ¸Ð¸ ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° IP3 Ñ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸ÐµÐ¼ Ð¼ÐµÑÐ¾Ð´Ð° ÑÐºÑÐ¿Ð°Ð½ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Application of the Expansion microscopy to IP3 clusterization analysis
Creators: name: Ð§Ð¸Ð³ÑÑÐ¹, ÐÐ°ÑÐ³Ð°ÑÐ¸ÑÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: ÑÐºÑÐ¿Ð°Ð½ÑÐ¸Ð¾Ð½Ð½Ð°Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ
IP3R
Bcl-2
Ð±Ð¾Ð»ÐµÐ·Ð½Ñ ÐÐ»ÑÑÐ³ÐµÐ¹Ð¼ÐµÑÐ°
Ca2+ ÑÐ¸Ð³Ð½Ð°Ð»Ð¸Ð½Ð³
ÐºÐ¾-Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ñ
ÐºÐ»Ð°ÑÑÐµÑÐ¸Ð·Ð°ÑÐ¸Ñ
expansion microscopy
Alzheimerâs disease
Ca2+ signaling
co-localization
clusterization

description: Ð­ÐºÑÐ¿Ð°Ð½ÑÐ¸Ð¾Ð½Ð½Ð°Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ â Ð¼ÐµÑÐ¾Ð´, Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÑÑÐ¸Ð¹ Ð¼Ð½Ð¾Ð³Ð¾ÐºÑÐ°ÑÐ½Ð¾ ÑÐ²ÐµÐ»Ð¸ÑÐ¸Ð²Ð°ÑÑ ÑÐ°Ð·Ð¼ÐµÑ Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼Ð¾Ð³Ð¾ Ð¾Ð±ÑÐ°Ð·ÑÐ°, Ð¿ÑÐ¸Ð¼ÐµÐ½ÑÐµÐ¼ÑÐ¹ Ð´Ð»Ñ ÑÐ»ÑÑÑÐµÐ½Ð¸Ñ ÐºÐ°ÑÐµÑÑÐ²Ð° Ð¸Ð·Ð¾Ð±ÑÐ°Ð¶ÐµÐ½Ð¸Ñ Ð¼Ð¸ÐºÑÐ¾- Ð¸ Ð½Ð°Ð½Ð¾-ÑÐ°Ð·Ð¼ÐµÑÐ½ÑÑ Ð¾Ð±ÑÐµÐºÑÐ¾Ð². IP3 ÑÐµÑÐµÐ¿ÑÐ¾ÑÑ ÑÐ²Ð»ÑÑÑÑÑ Ð¾Ð´Ð½Ð¸Ð¼Ð¸ Ð¸Ð· Ð¾ÑÐ½Ð¾Ð²Ð½ÑÑ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² Ð²ÑÑÐ²Ð¾Ð±Ð¾Ð¶Ð´ÐµÐ½Ð¸Ñ Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ ÐºÐ°Ð»ÑÑÐ¸Ñ Ð¸Ð· ÑÐ½Ð´Ð¾Ð¿Ð»Ð°Ð·Ð¼Ð°ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐµÑÐ¸ÐºÑÐ»ÑÐ¼Ð°. ÐÐ´Ð½Ð¾Ð¹ Ð¸Ð· ÑÐ°ÑÐ°ÐºÑÐµÑÐ½ÑÑ ÑÐµÑÑ Ð¿Ð°ÑÐ¾Ð³ÐµÐ½ÐµÐ·Ð° Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ ÐÐ»ÑÑÐ³ÐµÐ¹Ð¼ÐµÑÐ° (ÐÐ) ÑÐ²Ð»ÑÐµÑÑÑ Ð½Ð°ÑÑÑÐµÐ½Ð¸Ðµ ÑÐµÐ³ÑÐ»ÑÑÐ¸Ð¸ ÐºÐ°Ð»ÑÑÐ¸Ñ Ð² Ð½ÐµÐ¹ÑÐ¾Ð½Ð°Ñ, Ð¿Ð¾ÑÑÐ¾Ð¼Ñ ÑÐµÑÐµÐ¿ÑÐ¾Ñ IP3 ÑÐ²Ð»ÑÐµÑÑÑ Ð¿ÐµÑÑÐ¿ÐµÐºÑÐ¸Ð²Ð½Ð¾Ð¹ Ð¼Ð¸ÑÐµÐ½ÑÑ Ð´Ð»Ñ Ð±Ð¾ÑÑÐ±Ñ Ñ Ð´Ð°Ð½Ð½ÑÐ¼ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸ÐµÐ¼. Ð ÑÐ°Ð±Ð¾ÑÐµ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½ ÑÑÐ°Ð²Ð½Ð¸ÑÐµÐ»ÑÐ½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð· ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÐºÐ»Ð°ÑÑÐµÑÐ¾Ð² ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° IP3 Ð² Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ðµ Ð·Ð´Ð¾ÑÐ¾Ð²ÑÑ Ð¼ÑÑÐµÐ¹ Ð¸ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ðµ Ð¼ÑÑÐµÐ¹, Ð¼Ð¾Ð´ÐµÐ»Ð¸ÑÑÑÑÐ¸Ñ Ð±Ð¾Ð»ÐµÐ·Ð½Ñ ÐÐ»ÑÑÐ³ÐµÐ¹Ð¼ÐµÑÐ°. Ð ÑÐ°Ð±Ð¾ÑÐµ Ð¿ÑÐ¾Ð¸Ð·Ð²ÐµÐ´ÐµÐ½Ð° Ð¾ÑÐµÐ½ÐºÐ° ÐºÐ¾-Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° IP3 Ð¸ Ð±ÐµÐ»ÐºÐ° Bcl-2, ÑÐ°Ðº ÐºÐ°Ðº Ð¿Ð¾ÑÐ»ÐµÐ´Ð½Ð¸Ð¹ ÑÐ²Ð»ÑÐµÑÑÑ Ð²Ð°Ð¶Ð½ÑÐ¼ Ð¼Ð¾Ð´ÑÐ»ÑÑÐ¾ÑÐ¾Ð¼ Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐ¸ Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ ÐºÐ°Ð»ÑÑÐ¸Ñ Ð¸ ÑÐ¿Ð¾ÑÐ¾Ð±ÐµÐ½ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾Ð²Ð°ÑÑ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ðµ IP3 ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ°. Ð ÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ Ð¿Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÑÑ ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ðµ ÑÑÐ¾Ð²Ð½Ñ ÐºÐ¾-Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð±ÐµÐ»ÐºÐ° Bcl-2 Ð¸ ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° IP3 Ð² Ð³ÑÑÐ¿Ð¿Ðµ Ð¼ÑÑÐµÐ¹ Ñ ÐÐ, Ð° ÑÐ°ÐºÐ¶Ðµ Ð´ÐµÐ¼Ð¾Ð½ÑÑÑÐ¸ÑÑÑÑ ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ðµ ÑÑÐ¾Ð²Ð½Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ IP3R, Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÐ¸ Ð¸ ÑÐ°Ð·Ð¼ÐµÑÐ° ÐºÐ»Ð°ÑÑÐµÑÐ¾Ð² ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° IP3 Ð² Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ðµ Ð¼ÑÑÐµÐ¹, Ð¼Ð¾Ð´ÐµÐ»Ð¸ÑÑÑÑÐ¸Ñ Ð±Ð¾Ð»ÐµÐ·Ð½Ñ ÐÐ»ÑÑÐ³ÐµÐ¹Ð¼ÐµÑÐ°. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ ÑÐºÐ°Ð·ÑÐ²Ð°ÑÑ Ð½Ð° Ð²Ð°Ð¶Ð½Ð¾ÑÑÑ Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° IP3 Ð² ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐµ ÑÐµÑÐ°Ð¿ÐµÐ²ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¿Ð¾Ð´ÑÐ¾Ð´Ð° Ð´Ð»Ñ Ð»ÐµÑÐµÐ½Ð¸Ñ Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ ÐÐ»ÑÑÐ³ÐµÐ¹Ð¼ÐµÑÐ°.
description: Expansion microscopy is a method that allows to increase the size of the sample in many times; it is used to improve the image quality of micro- and nano- sized objects. IP3 receptors are one of the main channels for the release of intracellular calcium from the endoplasmic reticulum. One of the commonly accepted hallmarks of the pathogenesis of Alzheimers disease (AD) is the dysregulation of calcium in neurons, so the IP3 receptor is a promising target to fight against this disease. In this work, a comparative analysis of the formation of IP3 receptor clusters in the hippocampus of wild-type mice and the hippocampus of mouse model of Alzheimerâs disease was carried out. In this work, the co- localization of the IP3 receptor and the Bcl-2 protein was assessed, since the latter is an important modulator of intracellular calcium dynamics and it is able to inhibit the action of IP3 receptor. The results of this work indicate an increase in the level of co-localization of Bcl-2 protein and IP3 receptor in the group of mice with AD, as well as confirm an increase in the expression level of the IP3 receptor, density and size of IP3R clusters in the hippocampus of mice modeling Alzheimers disease. These results indicate the importance of studying the IP3 receptor in the development of a therapeutic approach for the treatment of Alzheimers disease.