Additional file 1 of Electroacupuncture protective effects after cerebral ischemia are mediated through miR-219a inhibition
Creators: name: Dai, Yaling
name: Wang, Sinuo
name: Yang, Minguang
name: Zhuo, Peiyuan
name: Ding, Yanyi
name: Li, Xiaoling
name: Cao, Yajun
name: Guo, Xiaoqin
name: Lin, Huawei
name: Tao, Jing
name: Chen, Lidian
name: Liu, Weilin
Publisher: name: figshare
Year: 2023
Subjects: subject: Biochemistry
subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Neuroscience
subject: Physiology
subject: Pharmacology
subject: Mental Health

description: Figure S1. EA could improve the cognitive function of MCAO rats A. Schematic representation of electroacupuncture points. B. Timeline and stages of the intervention design. C. Neurological deficit scores before treatment. D. Neurological deficit scores 24 hours after treatment. E. Neurological deficit scores 7 days after treatment. F. Neurological deficit scores 14 days after treatment. G. Results from the step-down avoidance test for each group before treatment. H. Results from the step-down avoidance test for each group 14 days after treatment. I. Escape latency in the Morris water maze test in each group. J. Morris water maze - visible platform task. K. Percentage of time spent in the target quadrant. *VS Sham group, P&lt;0.05,**, P&lt;0.01. #,VS 2-VO group, P&lt;0.05. ^,VS EA group, P&lt;0.05.(C-H, J-K,One-way ANOVA, followed by Tukey?s post hoc test, Data represent the mean ± SD. n= 18 rat /group, I . C. Repeated Measures Analysis of Variance, RM ANOVA, followed by Tukey?s post hoc test, n=10 rats/group, Data represent the mean ± SD). Figure S2. Effect of EA on cerebral infarction in MCAO rats. A. Size quantification of the infarcted brain region in MCAO group. B. Size quantification of the infarcted brain region in EA group. C. Size quantification of the infarcted brain region in Non-acu group. D. Determination of the infarct area in each group. (the dotted line shows the boundary of hippocampus brain regions) *. Sham vs MCAO, #.MCAO vs EA , ^.EA vs Non-acu. P&lt;0.05.( D. One-way ANOVA, followed by Tukey?s post hoc test, n= 18 rat /group, Data represent the mean ± SD). Figure S3 The cognitive function of PSCI is negatively correlated with the expression of miR-219a. A. Identification of serum miRNA signature change in PSCI and PSCIND group. B. Correlation analysis was conducted between the levels of miR-219a and MMSE score was identified. *, VS PSCIND group , P&lt;0.05,**, P&lt;0.01.(A. Independent t test., PSNCI group n=10, PSCI group=9; B linear correlation analysis?Data represent the mean ± SD?PSNCI group n=10, PSCI group=9). Figure S4. miR-219a negatively regulates NMDAR and affects learning and memory A. Escape latency in the Morris water maze test in WT and WT+miR-219a group. B. Morris water maze - visible platform task. C. The percentage of time spent in the target quadrant. D-E. Anxiety levels were measured using the elevated plus maze. F-G. OFT refers to Open Field Test. Time in the center measures anxiety levels. WT: wild type NS: non-significant.(A. Repeated Measures Analysis of Variance, RM ANOVA, B-G. Independent t test. n = 5 rats/ group, followed by Tukey?s post hoc test,). figure S5 flowchart of the experimental design