Data for: Assessment of cryogenic pretreatment for simulating environmental weathering in the formation of surrogate micro- and nanoplastics from agricultural mulch film
Creators: name: Astner, Anton
name: O'Neill, Hugh
name: Evans, Barbara
name: Pingali, Sai Venkatesh
name: Urban, Volker
name: Schaeffer, Sean
name: Young, Timothy
name: Hayes, Douglas
Publisher: name: Zenodo
Year: 2023
Subjects: Nanoplastics
Microplastics
Biodegradable Mulch films
biodegradation
polybutylene co-adipate- co-terephthalate (PBAT)
polylactic acid (PLA)
plastic pollution

description: Microplastics (MPs) and nanoplastics (NPs) from mulch films and other plastic materials employed in vegetable and small fruit production pose a major threat to agricultural ecosystems. For conducting controlled studies on MPs' and NPs' (MNPs') ecotoxicity to soil organisms and plants and fate and transport in soil, surrogate MNPs are<br>required that mimic MNPs that form in agricultural fields. We have developed a procedure to prepare MPs from plastic films or pellets using mechanical milling and sieving, and conversion of the resultant MPs into NPs through wet grinding, both steps of which mimic the degradation and fragmentation of plastics in nature. The major goal of this study was to determine if cryogenic exposure of two biodegradable mulch films effectively mimics the embrittlement caused by environmental weathering in terms of the dimensional, thermal, chemical, and biodegradability properties of the formed MNPs. We found differences in size, surface charge, thermal and chemical properties, and biodegradability in soil between MNPs' prepared from cryogenically treated vs. environmentally weathered films, related to the photochemical reactions occurring in the environment that were not mimicked by cryogenic treatment, such as depolymerization and cross-link formation. We also investigated the size reduction process for NPs and found that the size distribution was bimodal, with populations centered at 50 nm and 150–300 nm, and as the size reduction process progressed, the former subpopulation's proportion increased. The biodegradability of MPs in soil was greater than for NPs, a counter-intuitive trend since greater surface area exposure for NPs would increase biodegradability. The result is associated with differences in surface and chemical properties and to minor components that are readily leached out during the formation of NPs. In summary, the use of weathered plastics as feedstock would likely produce MNPs that are more realistic than cryogenically-treated unweathered films for use in experimental studies.
description: Only Microsoft Excel is required for opening the data files.Funding provided by: U.S. Department of Agriculture*<br>Crossref Funder Registry ID: <br>Award Number: 2020-67019-31167
description: The dataset was collected using scanning electron microscopy, dynamic light scattering, thermogravimetric analysis, and the ASTM D5988 standardized test for measuring the biodegradability of plastics under ambient soil conditions. JMP software was used to prepare ta model curve fit to the size distribution data for nanoplastics. Data have not been processed beyond what was provided by the instrumenton, except for the size of microplastics, which was determined from electron micrographs using ImageJ software.