cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Agrobacterium rhizogenes-mediated transformation of Arachis hypogaea: an efficient tool for functional study of genes
Creators: name: Shuai Liu
name: Liangchen Su
name: Xiaojun Zeng
name: Danmin Zheng
name: Hong, Lan
name: Li, Ling
Publisher: name: Taylor & Francis
Year: 2018
Subjects: subject: Space Science
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Plant Biology

description: We have developed a technique for efficient transformation of hairy roots of Arachis hypogaea L. using Agrobacterium rhizogenes K599, and have validated this approach for the investigation of gene function. As a model transgene, AhAREB1, a drought-resistance gene from peanut, was fused to green fluorescent protein, and four parameters that might influence the transformation efficiency were tested. The optimal procedure involved the use of petioles with four expanded leaves as explants, infection by K599 at optical density (OD600) of 0.6 for 15 min and co-cultivation for 2 d, giving transformation efficiencies of up to 91%. Hairy roots from transgenic peanut plants overexpressing AhAREB1 were unaffected by treatment with polyethylene glycol (PEG), demonstrating increased drought tolerance, whereas control roots showed clear signs of plasmolysis. Transgenic roots accumulated less superoxide anion (O2 −) than control roots under drought conditions. Additionally, transgenic roots displayed upregulation of four stress-response genes encoding WRKY transcription factor (WRKY33), MYB transcription factor (MYB92), abscisic acid receptor (PYL5) and dehydrin 2 (DHN2).
Agrobacterium rhizogenes-mediated transformation of Arachis hypogaea: an efficient tool for functional study of genes
Creators: name: Shuai Liu
name: Liangchen Su
name: Xiaojun Zeng
name: Danmin Zheng
name: Hong, Lan
name: Li, Ling
Publisher: name: Taylor & Francis
Year: 2016
Subjects: subject: Space Science
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Plant Biology

description: We have developed a technique for efficient transformation of hairy roots of Arachis hypogaea L. using Agrobacterium rhizogenes K599, and have validated this approach for the investigation of gene function. As a model transgene, AhAREB1, a drought-resistance gene from peanut, was fused to green fluorescent protein, and four parameters that might influence the transformation efficiency were tested. The optimal procedure involved the use of petioles with four expanded leaves as explants, infection by K599 at optical density (OD600) of 0.6 for 15 min and co-cultivation for 2 d, giving transformation efficiencies of up to 91%. Hairy roots from transgenic peanut plants overexpressing AhAREB1 were unaffected by treatment with polyethylene glycol (PEG), demonstrating increased drought tolerance, whereas control roots showed clear signs of plasmolysis. Transgenic roots accumulated less superoxide anion (O2−) than control roots under drought conditions. Additionally, transgenic roots displayed upregulation of four stress-response genes encoding WRKY transcription factor (WRKY33), MYB transcription factor (MYB92), abscisic acid receptor (PYL5) and dehydrin 2 (DHN2).
Vernetzung von Unternehmen und Forschungseinrichtungen in regionalen Innovationssystemen durch Webscraping
Creators: name: Meub, Lukas
name: Proeger, Till
name: Bizer, Kilian
name: Lahner, Jörg
Publisher: name: Göttingen : ifh, Volkswirtschaftliches Institut für Mittelstand und Handwerk an der Universität Göttingen
Year: 2022
Subjects: Regionale Innovationssysteme
Webscraping
Wirtschaftsförderung

description: Diese Studie zeigt am Beispiel von Südniedersachsen, wie mit der Methodik des Webscrapings effizient Informationen zu regionalen Akteuren wie Forschungseinrichtungen und Unternehmen gewonnen werden können. Webscraping beschreibt das systematische Auslesen der Inhalte von Webseiten und deren anschließende statistischer Analyse z.B. im Hinblick auf spezifische Technologien oder Strukturmerkmale von Unternehmen. Auf diesem Wege können gemeinsame technologische Schwerpunkte identifiziert werden, wodurch effizient Netzwerkaktivitäten und Projektverbünde zwischen den Akteuren aufgebaut werden können. Akteuren der Wirtschafts- und Innovationsförderung bietet dies ein innovatives Werkzeug, um die regionalen Innovationsnetzwerke strukturiert auszubauen und neue Kooperationen zu etablieren. Die Studie skizziert verschiedene Anwendungsmöglichkeiten am Beispiel von Südniedersachsen. Zum einen werden regionale Spezialisierungen bei Unternehmen und Forschungseinrichtungen in den Bereichen Wasserstoff und Lasertechnologie aufgezeigt. Ferner erfolgt eine Zuordnung von Unternehmen und Forschungseinrichtungen zu verschiedenen Technologieschwerpunkten. Übergreifend stellt Webscraping ein innovatives und effizient einsetzbares Instrument für regionale Innovationsakteure dar, das die regionale Koordination und den strukturierten, themenbezogenen Ausbau von Innovationsnetzwerken erleichtert.
description: Göttinger Beiträge zur Handwerksforschung ; 62
Fusione tra Vescicole Come Modello di Processi Cellulari
Creators: name: Caschera, Filippo
Publisher: name: figshare
Year: 2016
Subjects: subject: 60101 Analytical Biochemistry
subject: FOS: Biological sciences
subject: Biophysics
subject: Biotechnology
subject: Biological Techniques

description: Lo scopo di questa tesi è lo studio della fusione tra vescicole, come modello per la fusione di cellule e altri compartimenti biologici. La fusione tra compartimenti intracellulari e tra cellule è un evento fisiologico frequente che determina una varietà di processi cellulari di grande rilevanza. L’unione dei gameti, la fusione dei mioblasti, il traffico vescicolare, l’endocitosi e l’esocitosi, la trasmissione sinaptica (solo per citare i casi più noti) sono tutti processi nei quali la fusione tra membrane lipidiche esercita un ruolo chiave. Nelle cellule, la fusione tra membrane è altamente controllata da sistemi proteici il cui meccanismo d’azione (a livello molecolare) non è ancora completamente noto. Dal punto di vista biofisico, tuttavia, è lecito domandarsi se la fusione tra compartimenti separati da membrane possa avvenire sotto il controllo delle sole forze chimico-fisiche, che hanno verosimilmente dominato i processi di fusione tra protocellule in tempi prebiotici o comunque prima dello sviluppo dei meccanismi biomolecolari che osserviamo oggi. La fusione tra vescicole o liposomi convenzionali non è un processo spontaneo, anzi non si osserva affatto in condizioni normali. Una possibile strategia di avviamento della fusione vescicolare è basata su precursori elettrostabili di segno opposto. A tal scopo, in questa tesi si è studiata la reattività di vescicole lipidiche recanti carica opposta (vescicole anioniche e cationiche). Vescicole cationiche a base di didodecil dimetil ammonio bromuro (DDAB) sono fatte reagire con vescicole anioniche a base di acido oleico, al fine di determinare se – e in quali condizioni – la fusione possa aver luogo. Attraverso studi di turbidimetria, dynamic light scattering (DLS), potenziale zeta e fluorescenza si è dimostrato che la fusione tra vescicole è un processo possibile e che soggiace a un delicato equilibrio di forze contrapposte. In particolare, vescicole cationiche (POPC/DDAB 50/50) reagiscono con vescicole anioniche (POPC/oleato 20/80) con una resa di fusione fino al 25%, dipendente dal rapporto tra i reagenti. Dall’analisi dei dati è possibile ipotizzare un meccanismo fusione transiente, simile a quanto noto in neurofisiologia come modello kiss-and-run di rilascio sinaptico. I risultati sono analizzati per mostrare se, e in quale misura, lo studio della fusione tra vescicole sia un utile modello per comprendere la fusione delle membrane cellulari; ed anche per mettere in luce la possibile rilevanza per le metodologie relative alla veicolazione di farmaci e alla terapia genica.
Production of high activity Aspergillus niger BCC4525 β-mannanase in Pichia pastoris and its application for mannooligosaccharides production from biomass hydrolysis
Creators: name: Piyanun Harnpicharnchai
name: Waraporn Pinngoen
name: Wanwisa Teanngam
name: Warasirin Sornlake
name: Kittapong Sae-Tang
name: Pennapa Manitchotpisit
name: Sutipa Tanapongpipat
Publisher: name: Taylor & Francis
Year: 2016
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: Science Policy
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: A cDNA encoding β-mannanase was cloned from Aspergillus niger BCC4525 and expressed in Pichia pastoris KM71. The secreted enzyme hydrolyzed locust bean gum substrate with very high activity (1625 U/mL) and a relatively high kcat/Km (461 mg−1 s−1 mL). The enzyme is thermophilic and thermostable with an optimal temperature of 70 °C and 40% retention of endo-β-1,4-mannanase activity after preincubation at 70 °C. In addition, the enzyme exhibited broad pH stability with an optimal pH of 5.5. The recombinant enzyme hydrolyzes low-cost biomass, including palm kernel meal (PKM) and copra meal, to produce mannooligosaccharides, which is used as prebiotics to promote the growth of beneficial microflora in animals. An in vitro digestibility test simulating the gastrointestinal tract system of broilers suggested that the recombinant β-mannanase could effectively liberate reducing sugars from PKM-containing diet. These characteristics render this enzyme suitable for utilization as a feed additive to improve animal performance. A recombinant β-mannanase from A. niger BCC4525 exhibits high activity. It produces MOS from low-cost biomass and enhance the release of reducing sugars from diet.
The Human-World Relation Mediated by Images
Creators: name: Carvalho, Diana Ponciano Osório De
Publisher: name: Journal of Science and Technology of the Arts
Year: 2024

description: In the relation between human and machine vision, as mediators of a world understanding, there are differences associated with the subjective point of view (inherent to humans) and the objective point of view (inherent to machines). How machine vision contributes to our global awareness, even if it represents perspectives that are not accessible to us? The use of technologies thus influences our knowledge and, although we can obtain answers and solutions through them, such as when we use artificial intelligence, the meaning of this knowledge always requires a creative approach, a critical thinking. The aim is to question the degree to which these aspects can be present in artistic processes and the importance of the subjective gaze.
description: Journal of Science and Technology of the Arts, v. 15 n. 2 (2023): Arts and Gaming, Convergent Feminism and Speculative Futures
Exploring Empathy and Intersectionality in Gaming: a Case Study of Sweetxheart (Small, 2019)
Creators: name: Sousa, Carla
Publisher: name: Journal of Science and Technology of the Arts
Year: 2024

description: This study examines the ability of the game SweetXHeart – a genre-defying slice-of-life creation by Catt Small (2019) – to promote empathy and consciousness of microaggressions, specifically among participants whose characteristics differ from the game's main character – a white male-dominated sample. The study included 39 undergraduate students from a Bachelor’s Degree in videogames, offering a distinct chance to examine the reactions of this specific demographic to feminist and intersectional ideas in gaming.  After playing the game, participants were surveyed, and a mixed-methods approach, including quantitative and content analysis, was applied to their answers. The obtained results emphasize how players gain empathy by adopting the game character’s perspective and experiencing her life, and fostering connections. However, the game’s effectiveness in raising awareness depends on the participants’ understanding of intersectionality and the challenges faced by people in positions of privilege. 
description: Journal of Science and Technology of the Arts, v. 15 n. 2 (2023): Arts and Gaming, Convergent Feminism and Speculative Futures
title: The Isolation and Identification of Polyphenolics from Grape Seeds and Their Activity Towards Specific Cell Lines
Creators: name: Reddy, Joseph A.
Publisher: name: Old Dominion University
publisherIdentifier: https://ror.org/04zjtrb98
publisherIdentifierScheme: ROR
Year: 1993

description: Grape seed extract is being marketed in France for cardiovascular use and skin care. The active constituents of both these drugs are certain polyphenols called procyanidins. A similar seed extract has now been obtained from Virginia Chardonnay grape seeds. Ten of the major components in this extract have been isolated in their pure form by the semi-preparative HPLC separation on a YMC ODS-AQ (Octadecylsilane-AQ) column. The ODS-Aq column has been shown to have a better resolving capacity compared to the Spherisorb ODS (Octadecylsilane) column previously described in the literature. This has been attributed to the ODSAQ support which has both a hydrophobic high carbon loading and a relatively hydrophilic surface. By using published HPLC and TLC methods, six procyanidin dimers and three trimers have been identified from this extract.

Treatment of human fibroblast cell cultures with several of these compounds has shown that they have a growth inhibitory effect on these cells. Similar treatment of HL-60 tumor cell lines with these same procyanidins did not have any inhibition on their growth pattern. Procyanidins are known free radical scavengers and the same fractions have been tested for their protective influence on fibroblast cells against free radical induced damage. Hypoxanthine/xanthine oxidase induced free radical damage was found to be greater with cells treated with procyanidins than with untreated cells grown in MEM/10% BS media. Studies with low levels of serum in media showed some protective influence of these compounds against free radical damage of fibroblast cells. They were also tested for their ability to inhibit xanthine oxidase. No inhibition of xanthine oxidase by procyanidins up to a concentration of 200 pM was observed.
Video Games Against the Male Gaze
Creators: name: Lavrador, Ema
Publisher: name: Journal of Science and Technology of the Arts
Year: 2024

description: Video games are a medium that creates content often based on male gaze, allowing heteronormative and patriarchal portrayals that limit the representation of social minorities. In this article, we will study how video games counter the male gaze through realistic, diverse, and in-depth portrayals of their main characters. The case studies will be the characters Chloe Price from Life is Strange: Before the Storm (2017) by Deck Nine, Ellie and Abby from The Last of Us Part II (2020) by Naughty Dog, and Billie Lurk from Dishonored: Death of the Outsider (2017) by Arkane Studios. These video games feature strong female main characters who go against social norms and represent a diversity of sexuality, bodies, and cultures. Through this article, we intend to understand how these video games, which belong to series for a mass audience, are a step towards a commonplace of acceptance, accessibility, and awareness.


 
description: Journal of Science and Technology of the Arts, v. 15 n. 2 (2023): Arts and Gaming, Convergent Feminism and Speculative Futures
Exact models for the orthogonal delineation of site-specific management zones problem
Creators: name: Vicencio-Medina, Salvador J.
name: Quezada, Franco
name: Cid-Garcia, Nestor M.
name: Rojas, Omar J. Ibarra
name: Rios Solis, Yasmin
Publisher: name: figshare
Year: 2024
Subjects: classificationCode: 490108
subject: Operations research
classificationCode: 300202
subject: Agricultural land management
classificationCode: 490304
subject: Optimisation
classificationCode: 300204
subject: Agricultural management of nutrients

description: Precision agriculture is a concept that has been adopted worldwide to improve agricultural yields by effectively managing different decisions during the precision agriculture cycle. One of the first stages of precision agriculture is delineating the agricultural fields to improve both economic and ecological outcomes in agriculture. The objective of the Site-Specific Management Zone problem is to minimize the number of zones in the fields where each zone must be homogeneous to a soil property so that zones are maneuverable for agricultural machinery. To our knowledge, only heuristics have been proposed to solve this problem with orthogonal management zones. Indeed, one of the main difficulties is integrating the connexity of the orthogonal zones. Thus, this work proposes three mixed integer linear programming mathematical models and one constraint programming model that provide solutions with orthogonal zones and reach the agricultural homogeneous requirement. The proposed models have been tested with a benchmark presented in the literature for the Site-Specific Management Zone problem and compared with the literature heuristic methods. The experimental results show that the models provide high-quality results for small and medium instances.
Instructions and Reflections on How to Play with Time: an Illustrated Story Around the Futuroscopio
Creators: name: Alatorre Guzmán, Diego
Publisher: name: Journal of Science and Technology of the Arts
Year: 2024

description: This contribution delves into the philosophy and design process behind Futuroscopio, a tabletop roleplay game aimed at recovering the players’ agency to transform their present. The article charts the multiple prototypes and insights gained along its iterative development across manifold scenarios. Upon assessment, Futuroscopio emerged as a tool fostering exploration, collaboration, and dialogue, adept at addressing weighty subjects while maintaining players’ engagement. Aligned with Farnè’s Pedagogy of Play, this experience embodies the two fundamental states of education: a spontaneous immersion in natural chaos and a structured reflective process that leads players to learn what they need at their own pace. The conclusions point into an unexplored territory, where play is not limited to basic education, but entails an open attitude for discovery across multiple professional fields, therefore transversal to disciplinary thinking; where games act as provocations to imagine alternative scenarios and transform our present into better possible places.
description: Journal of Science and Technology of the Arts, v. 15 n. 2 (2023): Arts and Gaming, Convergent Feminism and Speculative Futures
Overheating in care settings: magnitude, causes, preparedness and remedies
Creators: name: Rajat Gupta
name: Barnfield, Laura
name: Gregg, Matt
Publisher: name: Taylor & Francis
Year: 2016
Subjects: subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Science Policy

description: Research in UK and elsewhere has highlighted that older people are particularly vulnerable to negative health effects of overheating. This paper examines the magnitude, causes, preparedness and remedies for addressing the risk of summertime overheating in four case study residential care and extra-care settings across the UK, spanning different building types, construction and age. An interdisciplinary approach is adopted, drawing from building science and social science methods, including temperature monitoring, building surveys, and interviews with design and management teams. The findings suggest that overheating is a current and prevalent risk in the case study schemes, yet currently little awareness or preparedness exists to implement suitable and long-term adaptation strategies (e.g., external shading). There was a perception from designers to managers, that cold represents a bigger threat to older occupants’ health than excessive heat. A lack of effective heat management was found across the case studies that included unwanted heat gains from the heating system, confusion in terms of responsibilities to manage indoor temperatures, and conflicts between window opening and occupant safety. Given that care settings should provide protection against risks from cold and hot weather, design, management and care practices need to become better focused towards this goal.
A Mani-Pedi-Anti-Counter-FESTO for Queer Screen Production Practice
Creators: name: Black, Angie
name: Kelly, Patrick
name: Munro, Kim
name: Taylor, Stayci
Publisher: name: Journal of Science and Technology of the Arts
Year: 2024

description: In this audiovisual essay, four practitioner-academics seek to identify and address the need to reimagine queer screen production. Traditional heteronormative storytelling dominates the screen production landscape, necessitating a challenge to create more inclusive and diverse narratives. Through the creation of a manifesto essay film, the researchers collectively reflect on their creative practices, synthesize their approaches, and develop a new vision for queer screen production. The result demonstrates the value of embracing: sustainable practices, queer kinship-making as filmmaking, alternatives to hegemonic forms, queer shame, queer failure, eternal adolescence, and the disruption of the ever-forward momentum (among other approaches). Manifesto-making as a method encourages creative practitioners to question the status quo of screen production contexts and strategies, and to think critically about the storytelling norms in broader creative practice. The researchers argue that such an approach can enable creative practitioners to pave the way for new, innovative collaborations and contribute to a more inclusive and diverse creative landscape.


This film enacts the opportunities that arise when considering the spectrum of screen production in broader, ‘queerer’, ways, through notions of kinship-making, polyphony and the ‘queer art of failure’ (Halberstam 2011). The disruption of dominant narrative models can be considered in the context of queer theory’s critiques of heteronormative temporality, asking how queer approaches to narrative construction might challenge the heteronormative markers of success and happiness, or what Elizabeth Freeman calls ‘chrononormativity’ (2010). Using ‘manifesto as method’, the film combines the authors’ separate practices in filmmaking, screenwriting, mobile media and documentary in ways that deviate from mainstream categorisations, production hierarchies and workflows.
description: Journal of Science and Technology of the Arts, v. 15 n. 2 (2023): Arts and Gaming, Convergent Feminism and Speculative Futures
Identification of mantle peridotite as a possible Iapetan ophiolite sliver in south Shetland, Scottish Caledonides
Creators: name: Day, James M.D.
name: O'Driscoll, Brian
name: Strachan, Rob
name: Daly, Stephen
name: Walker, Richard
Publisher: name: Geological Society of London
Year: 2016
Subjects: subject: Geology
subject: FOS: Earth and related environmental sciences

description: Supplementary information, including methods, supplementary figures and tabulated data.
Regulatory ecotoxicity testing of nanomaterials – proposed modifications of OECD test guidelines based on laboratory experience with silver and titanium dioxide nanoparticles
Creators: name: Hund-Rinke, Kerstin
name: Baun, Anders
name: Cupi, Denisa
name: Fernandes, Teresa F.
name: Handy, Richard
name: Kinross, John H.
name: Navas, José M.
name: Peijnenburg, Willie
name: Schlich, Karsten
name: Shaw, Benjamin J.
name: Janeck J. Scott-Fordsmand
Publisher: name: Taylor & Francis
Year: 2016
Subjects: subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: Inorganic Chemistry

description: Regulatory ecotoxicity testing of chemicals is of societal importance and a large effort is undertaken at the OECD to ensure that OECD test guidelines (TGs) for nanomaterials (NMs) are available. Significant progress to support the adaptation of selected TGs to NMs was achieved in the context of the project MARINA (http://www.marina-fp7.eu/) funded within the 7th European Framework Program. Eight OECD TGs were adapted based on the testing of at least one ion-releasing NM (Ag) and two inert NMs (TiO2). With the materials applied, two main variants of NMs (ion releasing vs. inert NMs) were addressed. As the modifications of the test guidelines refer to general test topics (e.g. test duration or measuring principle), we assume that the described approaches and modifications will be suitable for the testing of further NMs with other chemical compositions. Firm proposals for modification of protocols with scientific justification(s) are presented for the following tests: growth inhibition using the green algae Raphidocelis subcapitata (formerly: Pseudokirchneriella subcapitata; TG 201), acute toxicity with the crustacean Daphnia magna (TG 202), development toxicity with the fish Danio rerio (TG 210), reproduction of the sediment-living worm Lumbriculus variegatus (TG 225), activity of soil microflora (TGs 216, 217), and reproduction of the invertebrates (Enchytraeus crypticus, Eisenia fetida, TGs 220, 222). Additionally, test descriptions for two further test systems (root elongation of plants in hydroponic culture; test on fish cells) are presented. Ecotoxicological data obtained with the modified test guidelines for TiO2 NMs and Ag NM and detailed method descriptions are available.
In vitro assessment of TAT — Ciliary Neurotrophic Factor therapeutic potential for peripheral nerve regeneration
Creators: name: SENTHILKUMAR RAJENDRAN
Publisher: name: figshare
Year: 2016
Subjects: subject: 110502 Dental Therapeutics, Pharmacology and Toxicology
subject: FOS: Clinical medicine

description: In regenerative neurobiology, Ciliary Neurotrophic Factor (CNTF) is raising high interest as a multifunctional neurocytokine, playing a key role in the regeneration of injured peripheral nerves. Despite its promising trophic and regulatory activity, its clinical application is limited by the onset of severe side effects, due to the lack of efficient intracellular trafficking after administration. In this study, recombinant CNTF linked to the transactivator transduction domain (TAT) was investigated in vitro and found to be an optimized fusion protein which preserves neurotrophic activity, besides enhancing cellular uptake for therapeutic advantage. Moreover, a compelling protein delivery method was defined, in the future perspective of improving nerve regeneration strategies. Following determination of TAT-CNTF molecular weight and concentration, its specific effect on neural SH-SY5Y and PC12 cultures was assessed. Cell proliferation assay demonstrated that the fusion protein triggers PC12 cell growth within 6 h of stimulation. At the same time, the activation of signal transduction pathway and enhancement of cellular trafficking were found to be accomplished in both neural cell lines after specific treatment with TAT-CNTF. Finally, the recombinant growth factor was successfully loaded on oxidized polyvinyl alcohol (PVA) scaffolds, and more efficiently released when polymer oxidation rate increased.Taken together, our results highlight that the TAT domain addiction to the protein sequence preserves CNTF specific neurotrophic activity in vitro, besides improving cellular uptake. Moreover, oxidized PVA could represent an ideal biomaterial for the development of nerve conduits loaded with the fusion protein to be delivered to the site of nerve injury.
In vitro assessment of TAT - Ciliary Neurotrophic Factor therapeutic potential for peripheral nerve regeneration.
Creators: name: SENTHILKUMAR RAJENDRAN
Publisher: name: figshare
Year: 2016
Subjects: subject: 110502 Dental Therapeutics, Pharmacology and Toxicology
subject: FOS: Clinical medicine

description: In regenerative neurobiology, Ciliary Neurotrophic Factor (CNTF) is raising high interest as a multifunctional neurocytokine, playing a key role in the regeneration of injured peripheral nerves. Despite its promising trophic and regulatory activity, its clinical application is limited by the onset of severe side effects, due to the lack of efficient intracellular trafficking after administration. In this study, recombinant CNTF linked to the transactivator transduction domain (TAT) was investigated in vitro and found to be an optimized fusion protein which preserves neurotrophic activity, besides enhancing cellular uptake for therapeutic advantage. Moreover, a compelling protein delivery method was defined, in the future perspective of improving nerve regeneration strategies. Following determination of TAT-CNTF molecular weight and concentration, its specific effect on neural SH-SY5Y and PC12 cultures was assessed. Cell proliferation assay demonstrated that the fusion protein triggers PC12 cell growth within 6 h of stimulation. At the same time, the activation of signal transduction pathway and enhancement of cellular trafficking were found to be accomplished in both neural cell lines after specific treatment with TAT-CNTF. Finally, the recombinant growth factor was successfully loaded on oxidized polyvinyl alcohol (PVA) scaffolds, and more efficiently released when polymer oxidation rate increased.Taken together, our results highlight that the TAT domain addiction to the protein sequence preserves CNTF specific neurotrophic activity in vitro, besides improving cellular uptake. Moreover, oxidized PVA could represent an ideal biomaterial for the development of nerve conduits loaded with the fusion protein to be delivered to the site of nerve injury.
Polskie czasopisma z nauk humanistycznych i nauk społecznych w liczbach
Creators: name: Rozkosz, Ewa
name: Kulczycki, Emanuel
Publisher: name: figshare
Year: 2016
Subjects: Science Policy

description: Raport zawiera dane naukometryczne na temat
 818 polskich czasopism naukowych z obszaru nauk humanistycznych i społecznych poddanych ewaluacji i umieszczonych na trzech Wykazach czasopism punktowanych opublikowanych w latach: 2012, 2013 i 2015. Analizowaliśmy zmiany punktacji poszczególnych czasopism jak również rozkład punktów dla czasopism przyporządkowanych do danej dyscypliny naukowej z obszaru nauk humanistycznych i społecznych. Uwzględniliśmy wyłącznie czasopisma znajdujące się we wszystkich trzech wykazach w części B. Nie uwzględniliśmy natomiast czasopism, które w trakcie tych lat były indeksowane na różnych częściach Wykazu czasopism punktowanych, np. w wykazie z 2013 r. znajdowały się w części C, a w 2015 r. zostały przeniesione do części B. Redakcje w ankiecie ewaluacyjnej wskazywały jedną lub dwie dyscypliny podstawowe, w ramach których funkcjonuje czasopismo. Dyscypliny były wskazywane zgodnie z Rozporządzeniem Ministra Nauki i Szkolnictwa Wyższego z dnia 8 sierpnia 2011 r. w sprawie obszarów wiedzy, dziedzin nauki i sztuki oraz dyscyplin naukowych i artystycznych (Dz.U. 2011 nr 179 poz. 1065). Dane: stan na 31 grudnia 2015 r.
Triterpenoids from Coluria longifolia
Creators: name: Gao, Yan-Ping
name: Liang, Jian
name: Guo-Yue Zhong
name: Luo, Yong-Ming
Publisher: name: Taylor & Francis
Year: 2016
Subjects: subject: Biochemistry
subject: Pharmacology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: FOS: Biological sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Cancer
subject: Plant Biology

description: A new triterpenoid named 19-hydroxy swinhoeic acid, together with four known compounds were isolated from the whole plants of Coluria longifolia Maxim. Their structures were elucidated on the basis of spectroscopic data analysis and comparison with previously reported data. Cytotoxicities of the five compounds against HepG2 cell lines were also evaluated, unfortunately, no obvious activities were observed.
Supplementary Material for: High Prevalence of Back Pain and Axial Spondyloarthropathy in Patients with Hidradenitis Suppurativa
Creators: name: Schneider-Burrus, S.
name: Witte-Haendel, E.
name: Christou, D.
name: Rigoni, B.
name: Sabat, R.
name: Diederichs, G.
Publisher: name: Karger Publishers
Year: 2016
Subjects: Medicine

description: Background: Hidradenitis suppurativa (HS) is a chronic inflammatory disease, causing fistulating sinuses in the intertriginous skin of axillary, genitofemoral and perianal sites. Objective: As other chronic inflammatory diseases, e.g. psoriasis, are frequently associated with spondyloarthropathies (SpA), the goal of this study was to quantify the prevalence of back pain and SpA in HS patients. Methods: A prospective questionnaire survey in 100 HS patients and a retrospective evaluation of pelvic magnetic resonance imaging (MRI) scans in 46 HS patients were conducted. Results: 71% of HS patients were suffering from back pain. There was no difference between age at onset of HS, disease duration, body mass index (BMI), or disease severity between HS patients with and without back pain. Evaluating pelvic MRI scans, 32.6% of HS patients showed signs of chronic SpA and 39.1% signs of active SpA. Again, no significant differences between patients with/without SpA were found regarding age at time of MRI, age at onset of HS, disease duration, smoking habits, and BMI. Furthermore, there was no correlation between these parameters and the degree of SpA. Limitations: Only patients with moderate/severe HS (Hurley stage II and III) in genitofemoral/perianal sites were analysed via MRI scans. Conclusion: Back pain and SpA are very common among patients with moderate/severe HS. Neither medical history nor clinical parameters provide hints for the presence of SpA.
title: Bakterielle Lebensmittelinfektionen durch Vibrionen: Gesundheitliche Bewertung zum Vorkommen von Vibrio spp. (Nicht-Cholera-Vibrionen) in Lebensmitteln: Stellungnahme Nr. 011/2022 des BfR vom 13. April 2022
Creators: name: Bundesinstitut Für Risikobewertung
Publisher: name: Bundesbehörden und Einrichtungen im Geschäftsbereich des Bundesministeriums für Ernährung und Landwirtschaft (BMEL)
Year: 2022
Subjects: subject: 000

description: BfR-Stellungnahmen, vol. 2022, no. 011
Maternal citrulline supplementation enhances placental function and fetal growth in a rat model of IUGR: involvement of insulin-like growth factor 2 and angiogenic factors
Creators: name: Nhat-Thang Tran
name: Amarger, Valerie
name: Bourdon, Aurelie
name: Misbert, Emilie
name: Grit, Isabelle
name: Winer, Norbert
name: Darmaun, Dominique
Publisher: name: Taylor & Francis
Year: 2016
Subjects: subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Physiology
subject: FOS: Biological sciences
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Developmental Biology
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Objective: To determine the effects of maternal citrulline supplementation on fetal growth and placental efficiency in a rat model of intrauterine growth restriction (IUGR) induced by maternal protein restriction. Methods: Pregnant Sprague–Dawley rats were randomly assigned to three groups: NP (receiving a control 20% protein diet), LP (a 4% protein diet), or LP-CIT (an LP diet along with L-citrulline, 2 g/kg/d in drinking water). On the 15th and 21st day of gestation (GD15 and GD21, respectively), dams underwent a C-section, by which fetuses and placentas were extracted. The expression of genes involved in placental growth and angiogenesis was studied by quantitative RT-PCR. Results: Maternal citrulline supplementation increased fetal weight at GD21, and fetal weight/placental weight ratio, an index of placental efficiency, from mid gestation (p Igf2-P0, a placenta-specific variant of insulin-like growth factor 2 (Igf2) gene, and Vegf and Flt-1, involved in angiogenic pathways, was enhanced in the LP-CIT group (versus NP, p p p Igf2-P0, Vegf, and Flt-1, respectively). Conclusions: In a model of IUGR induced by protein deprivation, citrulline enhances fetal growth, placental efficiency, and the expression of genes involved in angiogenesis. The relevance of such effect in human pregnancies complicated by IUGR warrants further study.
title: On a hyperbolic wave equation in unbounded domains
title: Μελέτη μιας υπερβολικής κυματικής εξίσωσης σε μη φραγμένα χωρία
titleType: AlternativeTitle
Creators: name: Στρατικόπουλος, Ελευθέριος
Publisher: name: Πανεπιστήμιο Δυτικής Αττικής
Year: 2022
Subjects: Infinite dimensional dynamical systems
Quasilinear hyperbolic wave equations
Banach fixed-point theorem
Semigroup theory
Galerkin method
Blow-up
Concavity method
Potential well

description: The aim of this thesis is the study of the quasilinear damped wave equation of Kirchhoff’s type with variable diffusion coefficient in all of R^N. For the functional analysis of the time dependent problem, we make use of the homogeneous Sobolev spaces and of the generalized Sobolev embeddings, followed by the preceding studies of the Kirchhoff’s type problem. In strong connection with the corresponding natural phenomena, we obtain results concerning the local (unique) existence of the solutions using the Faedo-Galerkin approximation and the Banach Fixed-Point Theorem. We also prove the global existence and energy estimates of the solutions using the method of the modified potential well. We complete our study with the blow-up analysis of the solutions for initial data of negative energy using the concavity method, where for the discrete case (a=2) we prove the modification of the upper-bound of the time T.
title: Transformer models for Greek and English language understanding
title: Μοντέλα μετασχηματιστών για την κατανόηση Ελληνικής και Αγγλικής γλώσσας
titleType: AlternativeTitle
Creators: name: Μαγειρίδης, Χαράλαμπος
Publisher: name: Πανεπιστήμιο Δυτικής Αττικής
Year: 2024
Subjects: Transformers
Sentiment analysis
NLP

description: This thesis examines the effectiveness of Transformer models in natural lan- guage processing (NLP), focusing on text classification and sentiment analysis for both Greek and English texts. It marks a significant advancement in NLP by demonstrating how Transformers, through their self-attention mechanisms, offer a superior approach to understanding context and relationships within language com- pared to traditional RNN architectures.Through a detailed exploration of several Transformer models, such as BERT, RoBERTa, and GPT-2, and their comparison with RNNs, this study evaluates their performance across different languages and text types. Fine-tuning language-specific pretrained versions of these models on two distinct datasets—Greek product reviews and English tweets related to the #metoo movement—allows for an examination of the adaptability of Transformers to diverse linguistic challenges. The research ultimately illustrates that Transformer models significantly outperform traditional RNNs in sentiment analysis, highlighting their considerable potential to enhance language understanding across varied linguistic and cultural contexts.
Impact of acute psychological stress on cardiovascular risk factors in face of insulin resistance
Creators: name: Jones, Kristian T.
name: Shelton, Richard C.
name: Wan, Jun
name: Li, Li
Publisher: name: Taylor & Francis
Year: 2016
Subjects: subject: Medicine
subject: Physiology
subject: FOS: Biological sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: Science Policy
subject: 111714 Mental Health
subject: FOS: Health sciences
subject: Computational Biology

description: Individuals with insulin resistance (IR) are at greater risk for cardiovascular disease (CVD). Psychological stress may contribute to develop CVD in IR, although mechanisms are poorly understood. Our aim was to test the hypothesis that individuals with IR have enhanced emotional and physiological responses to acute psychological stress, leading to increased CVD risk. Sixty participants were enrolled into the study, and classified into IR group (n = 31) and insulin sensitive group (n = 29) according to the Quantitative insulin sensitivity check index, which was calculated based on an oral glucose tolerance test. The Trier social stress test, a standardized experimental stress paradigm, was performed on each participant, and emotional and physiological responses were examined. Blood was collected from each subject for insulin, cytokines, and cortisol measurements. Compared with the insulin-sensitive group, individuals with IR had significantly lower ratings of energy and calm, but higher fatigue levels in response to acute stressors. Individuals with IR also showed blunted heart rate reactivity following stress. In addition, the IR status was worsened by acute psychological stress as demonstrated by further increased insulin secretion. Furthermore, individuals with IR showed significantly increased levels of leptin and interleukin-6, but decreased levels of adiponectin, at baseline, stress test, and post-stress period. Our findings in individuals with IR under acute stress would allow a better understanding of the risks for developing CVD and to tailor the interventions for better outcomes.
Cypripedium L. 1753
Creators: name: Landolt, Elias
Publisher: name: Zenodo
Year: 2001
Subjects: Biodiversity
Taxonomy
Plantae
Tracheophyta
Liliopsida
Asparagales
Orchidaceae
Cypripedium

description: CypripediumL. Frauenschuh
description: Published as part of Elias Landolt, 2001, Orchidaceae, pp. 339-364 in Flora der Stadt Zürich (1984 - 1998), Basel :Birckhäuser Verlag on page 340, DOI: 10.5281/zenodo.208186
Note Illustrative della Carta geologica d'Italia alla scala 1:50.000 F. 252 Barberino di Mugello, Servizio Geologico d'Italia - ISPRA
Creators: name: Bettelli, G.
name: Boccaletti, M.
name: Cibin, U.
name: Panini, F.
name: Poccianti, C.
name: Rosselli, S.
name: Sani, F.
Publisher: name: INFN Open Access Repository
Year: 2002
Subjects: carta geologica
Barberino di Mugello
Emilia-Romagna
Pianura Padana
CARG
stratigrafia
tettonica
sottosuolo
geological map
stratigraphy
tectonics
subsurface

description: Note illustrative redatte per il Foglio geologico n. 252 Barberino di Mugello della Carta Geologica d'Italia alla scala 1:50.000. 130 pp.
A series of 1-D, 2-D and 3-D coordination polymers self-assembled from a flexible dicarboxylate and mixed N-donor ligands: syntheses, structural diversity, and luminescent properties
Creators: name: Gao, Yan-Peng
name: Guo, Le
name: Lv, Yan-Hua
name: Dong, Wei
name: Jia, Min
name: Chang, Fei
Publisher: name: Taylor & Francis
Year: 2016
Subjects: subject: Biophysics
subject: Biochemistry
subject: 29999 Physical Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: Medicine
subject: Pharmacology
subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences

description: We have synthesized a series of metal–organic coordination frameworks under solvothermal conditions, formulated as [Cu(L)(phen)](1), [Co2(L)2(bib)]·CH3OH(2), [Co(L)(btmb)0.5](3), [Zn(L)(bib)]·2H2O(4), [Cu(L)(bib)]·DMF(5), and [Mn4(L)4(bimb)(CH3OH)](6) based on a flexible multicarboxylic bridging ligand 4,4′-methylenebis(oxy)-dibenzoic acid (H2L) and flexible N-donor ligands 1,4-bis (1H-imidazol-1-yl)-butane (bib), 1,4-bis(1H-1,2,4-triazol- 1-ylmethyl)benzene (btmb), and 1,4-bis(1H-imidazol-1-ylmethyl)benzene (bimb). The structures of the frameworks have been determined by single-crystal X-ray diffraction analyses and further characterized by elemental analyses, IR spectra, and thermogravimetric analyses. The crystal structure determinations reveal that 1 has a 1-D Z-shape chain. Compounds 2 and 3 are 2-D twofold parallel interpenetrating 4-connected net with the Schläfli symbol {44·62}. Compound 4 is a 2-D threefold parallel interpenetrating 4-connected nets with the Schläfli symbol {44·62}. Compound 5 is 3-D 4-connected net with the Schläfli symbol {65·8}. Compound 6 is characterized by a three-dimensional framework with one-dimensional homogeneous Mn-carboxylate chain. We found that flexible carboxylate ligands have different coordination modes under different synthetic conditions. The flexible skeleton of ligands and the coordination angle between the ligand and the metal ion is described. The luminescence and thermogravimetric properties of these compounds have been investigated.
Epipogium R. Br. 1810
Creators: name: Landolt, Elias
Publisher: name: Zenodo
Year: 2001
Subjects: Biodiversity
Taxonomy
Plantae
Tracheophyta
Liliopsida
Asparagales
Orchidaceae
Epipogium

description: Epipogium R. Br. Widerbart
description: Published as part of Elias Landolt, 2001, Orchidaceae, pp. 339-364 in Flora der Stadt Zürich (1984 - 1998), Basel :Birckhäuser Verlag on page 340, DOI: 10.5281/zenodo.208186
Epipogium aphyllum Sw. 1814
Creators: name: Landolt, Elias
Publisher: name: Zenodo
Year: 2001
Subjects: Biodiversity
Taxonomy
Plantae
Tracheophyta
Liliopsida
Asparagales
Orchidaceae
Epipogium
Epipogium aphyllum

description: Epipogium aphyllum (F. W. Schmidt) Sw. Widerbart Blüte. Gelblichgrün mit weisser Lippe und rötlichem bis gelblichem Sporn. 7-8. Standort. Kollin und montan. Basenarme, humusreiche Böden in schattigen Lagen. Buchen- und Nadelwäl­ der. F 3, R 2. N 2, H 5, D 4, L 1, T 3, K 3. W: g. Literatur. Zürichberg ( N aegeli 1917). Herbar. 7: Susenberg (o. D.). Verbreitung. Keine rezenten Fundstellen. Könnte vorübergehend angeweht werden. U: 1, H: 3/0, E: 1>0, W: I. - Europa, Westasien.
description: Published as part of Elias Landolt, 2001, Orchidaceae, pp. 339-364 in Flora der Stadt Zürich (1984 - 1998), Basel :Birckhäuser Verlag on pages 340-342, DOI: 10.5281/zenodo.208186