title: ÐÐ¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ñ Ð¼Ð¸ÑÐµÐ½Ð¸ ÐÐÐÐÐ ÑÑÑÑÐºÑÑÑÐ°Ð¼Ð¸ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ Ð´Ð¸Ð¾ÐºÑÐ¸Ð´Ð° ÑÐ¸ÑÐºÐ¾Ð½Ð¸Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Modification of the MALDI-target by structures based on zirconium dioxide
Creators: name: ÐÐ»ÑÑÑÐ¾Ð½Ð¾Ðº, Ð¡ÐµÐ¼ÑÐ½
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÑÐ¸ÑÐµÑÐºÐ¾Ðµ Ð¾ÑÐ°Ð¶Ð´ÐµÐ½Ð¸Ðµ (Ð­Ð¤Ð)
Ð±ÐµÑÐºÐ°Ð¿ÐµÐ»ÑÐ½Ð¾Ðµ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ°ÑÐ¿ÑÐ»ÐµÐ½Ð¸Ðµ
Ð¼ÐµÑÐ°Ð»Ð»-Ð°ÑÑÐ¸Ð½Ð½Ð°Ñ Ñ ÑÐ¾Ð¼Ð°ÑÐ¾Ð³ÑÐ°ÑÐ¸Ñ
ÑÐ¾ÑÐ±ÐµÐ½Ñ
ÑÐ¾ÑÑÐ¾Ð¿ÑÐ¾ÑÐµÐ¾Ð¼Ð¸ÐºÐ°
ÐÐÐÐÐ-Ð¼Ð¸ÑÐµÐ½Ñ
ÐÐÐÐÐ-Ð¼Ð°ÑÑ-ÑÐ¿ÐµÐºÑÑÐ¾Ð¼ÐµÑÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð°Ð½Ð°Ð»Ð¸Ð·
electrophoretic deposition (EPD)
drop-free electric spraying
metal affinity chromatography
metal oxide sorbents
phosphoproteomics
MALDI-target
MALDI-MS analysis

description: Ð Ð°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ Ð´Ð²ÑÑ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ ÐÐÐÐÐ-Ð¼Ð¸ÑÐµÐ½Ð¸ Ð¿Ð¾ÑÐ¸ÑÑÑÐ¼ Ð½Ð°Ð½Ð¾Ð´Ð¸ÑÐ¿ÐµÑÑÐ½ÑÐ¼ Ð¿Ð¾ÑÐ¾ÑÐºÐ¾Ð¼ Ð´Ð¸Ð¾ÐºÑÐ¸Ð´Ð° ÑÐ¸ÑÐºÐ¾Ð½Ð¸Ñ, Ð¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ ÑÐ¸Ð¿Ð° ÑÐ°ÑÑÐ¸Ñ Ð¼ÐµÑÐ°Ð»Ð»-Ð¾ÐºÑÐ¸Ð´Ð½Ð¾Ð³Ð¾ ÑÐ¾ÑÐ±ÐµÐ½ÑÐ° Ð½Ð° ÑÐµÐ·ÑÐ»ÑÑÐ°Ñ Ð¼ÐµÑÐ°Ð»Ð»-Ð°ÑÑÐ¸Ð½Ð½Ð¾Ð¹ ÑÑÐ¾Ð¼Ð°ÑÐ¾Ð³ÑÐ°ÑÐ¸Ð¸. ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: 1. ÐÐ¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ñ ÐÐÐÐÐ-Ð¼Ð¸ÑÐµÐ½Ð¸ Ð¿Ð¾ÑÐ¸ÑÑÑÐ¼ Ð´Ð¸Ð¾ÐºÑÐ¸Ð´Ð¾Ð¼ ÑÐ¸ÑÐºÐ¾Ð½Ð¸Ñ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð±ÐµÑÐºÐ°Ð¿ÐµÐ»ÑÐ½Ð¾Ð³Ð¾ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ°ÑÐ¿ÑÐ»ÐµÐ½Ð¸Ñ. 2. Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½Ð¾Ð¹ ÑÑÑÐ°Ð½Ð¾Ð²ÐºÐ¸ Ð´Ð»Ñ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¾ÑÐ°Ð¶Ð´ÐµÐ½Ð¸Ñ. 3. ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¸ Ð°Ð½Ð°Ð»Ð¸Ð· Ð´ÐµÑÐµÐºÑÐ¾Ð² ÑÐ»Ð¾Ñ, Ð¾ÑÐ°Ð¶Ð´ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¾ÑÐ°Ð¶Ð´ÐµÐ½Ð¸Ñ. 4. ÐÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ðµ Ð¼ÐµÑÐ°Ð»Ð»-Ð°ÑÑÐ¸Ð½Ð½Ð¾Ð¹ ÑÑÐ¾Ð¼Ð°ÑÐ¾Ð³ÑÐ°ÑÐ¸Ð¸ Ð½Ð° Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ ÐÐÐÐÐ- Ð¼Ð¸ÑÐµÐ½ÑÑ. Ð Ð°Ð±Ð¾ÑÐ° Ð±ÑÐ»Ð° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ Ð¤ÐÐÐ£Ð ÐÐ¢ Ð¤ÐÐÐ Ð Ð¾ÑÑÐ¸Ð¸. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð±ÑÐ»Ð° ÑÐ¾Ð±ÑÐ°Ð½Ð° ÑÑÑÐ°Ð½Ð¾Ð²ÐºÐ° Ð´Ð»Ñ Ð­Ð¤Ð, ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð° ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð°Ñ ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ñ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ Ð­Ð¤Ð, ÑÑÐ¾Ð²ÐµÑÑÐµÐ½ÑÑÐ²Ð¾Ð²Ð°Ð½Ð° ÑÑÑÐ°Ð½Ð¾Ð²ÐºÐ° Ð´Ð»Ñ ÑÐ»ÐµÐºÑÐ¾ÑÐ°ÑÐ¿ÑÐ»ÐµÐ½Ð¸Ñ Ð² Ð±ÐµÑÐºÐ°Ð¿ÐµÐ»ÑÐ½Ð¾Ð¼ ÑÐµÐ¶Ð¸Ð¼Ðµ, Ð¿Ð¾Ð´Ð³Ð¾ÑÐ¾Ð²Ð»ÐµÐ½Ð° Ð¼Ð¸ÑÐµÐ½Ñ Ð´Ð»Ñ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ Ð¼ÐµÑÐ°Ð»Ð»-Ð°ÑÑÐ¸Ð½Ð½Ð¾Ð¹ ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸, Ð¸ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð° Ð¿ÑÐ¸Ð½ÑÐ¸Ð¿Ð¸Ð°Ð»ÑÐ½Ð°Ñ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾ÑÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð¹ Ð¼Ð¸ÑÐµÐ½Ð¸ Ð´Ð»Ñ Ð±Ð¸Ð¾Ð¾ÑÐ³Ð°Ð½Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð°.
description: This work is devoted to comparing two methods for modifying a MALDI-target with zirconium dioxide, and to studying the effect of the particle size of the metal-oxide sorbent on the result of metal-affinity chromatography. Purpose: development of surface modification of the MALDI target with nano-dispersed zirconium dioxide powders in order to obtain a functional surface for metal affinity chromatography. Tasks that were solved during the research: 1. Modification of the MALDI target by porous zirconium dioxide by the method of drop-free electrospray. 2. Development of an experimental installation for electrophoretic deposition. 3. Investigation and analysis of defects of the layer deposited by electrophoretic deposition. 4. Carrying out metal affinity chromatography on modified MALDI targets The work was carried out on the basis of Â«Institute of Toxicology, Federal Medical- Biological Agency of RussiaÂ». As a result, an installation for EFD was assembled, the MALDI target was modified by two methods, and metal affinity chromatography was done. Photographs of the deposited zirconium dioxide layer were obtained by the ETF method and an efficient ETF technology was developed.