title: ÐÐ°ÑÑÑÐµÐ½Ð¸Ñ ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¿Ð»Ð°ÑÑÐ¸ÑÐ½Ð¾ÑÑÐ¸ Ð² Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ðµ ÐºÑÑÑ Ð² Ð»Ð¸ÑÐ¸Ð¹-Ð¿Ð¸Ð»Ð¾ÐºÐ°ÑÐ¿Ð¸Ð½Ð¾Ð²Ð¾Ð¹ Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð²Ð¸ÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Disorders of synaptic plasticity in the hippocampus of rats in the lithium-pilocarpine model of temporal epilepsy
Creators: name: ÐÐ¸ÐµÑÐ¿Ð¸ÑÐ¾Ð², ÐÐµÐ¾ÑÐ³Ð¸Ð¹
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: Ð²Ð¸ÑÐ¾ÑÐ½Ð°Ñ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ñ
Ð»Ð¸ÑÐ¸Ð¹-Ð¿Ð¸Ð»Ð¾ÐºÐ°ÑÐ¿Ð¸Ð½Ð¾Ð²Ð°Ñ Ð¼Ð¾Ð´ÐµÐ»Ñ
ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ°Ñ Ð¿Ð»Ð°ÑÑÐ¸ÑÐ½Ð¾ÑÑÑ
Ð´Ð¾Ð»Ð³Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð°Ñ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°ÑÐ¸Ñ
Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿
temporal lobe epilepsy
lithium-pilocarpine model
synaptic plasticity
long-term potentiation
hippocampus

description: ÐÐ°Ð¼Ð¸ Ð±ÑÐ» Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½ ÑÑÐ´ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹, Ð¿Ð¾Ð´ÑÐ²ÐµÑÐ¶Ð´Ð°ÑÑÐ¸Ñ, ÑÑÐ¾ Ð¿ÐµÑÐµÐ½ÐµÑÐµÐ½Ð½ÑÐµ ÑÑÐ´Ð¾ÑÐ¾Ð¶Ð½ÑÐµ ÑÐ¾ÑÑÐ¾ÑÐ½Ð¸Ñ Ð² Ð·Ð½Ð°ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ¿ÐµÐ½Ð¸ Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÑÑ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð½Ð° ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ¸ ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¿Ð»Ð°ÑÑÐ¸ÑÐ½Ð¾ÑÑÐ¸ Ð² Ð¿Ð¾Ð»Ðµ Ð¡A1 Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð° ÐºÑÑÑ. Ð¦ÐµÐ»ÑÑ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¾ Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐ¸ Ð¸ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð¾Ð² ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð´Ð¾Ð»Ð³Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð¹ ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°ÑÐ¸Ð¸ (ÐÐÐ) Ð² Ð¿Ð¾Ð»Ðµ CA1 Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð° ÐºÑÑÑ Ð² Ð»Ð°ÑÐµÐ½ÑÐ½ÑÑ ÑÐ°Ð·Ñ Ð»Ð¸ÑÐ¸Ð¹-Ð¿Ð¸Ð»Ð¾ÐºÐ°ÑÐ¿Ð¸Ð½Ð¾Ð²Ð¾Ð¹ Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð²Ð¸ÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ð¸ (ÐÐ­) Ñ ÐºÑÑÑ. ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»Ð¸ÑÑ ÑÐµÑÐµÐ· 1, 3, 7 Ð´Ð½ÐµÐ¹ Ð¿Ð¾ÑÐ»Ðµ Ð¿Ð¸Ð»Ð¾ÐºÐ°ÑÐ¿Ð¸Ð½-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÑÐ°ÑÑÑÐ° (Ð­Ð¡). Ð ÐµÐ³Ð¸ÑÑÑÐ°ÑÐ¸Ñ Ð¿Ð¾Ð»ÐµÐ²ÑÑ Ð²Ð¾Ð·Ð±ÑÐ¶Ð´Ð°ÑÑÐ¸Ñ Ð¿Ð¾ÑÑÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð¾Ð² (Ð¿ÐÐÐ¡Ð) Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»Ð¸ Ð½Ð° Ð¿ÐµÑÐµÐ¶Ð¸Ð²Ð°ÑÑÐ¸Ñ ÑÑÐµÐ·Ð°Ñ Ð¼Ð¾Ð·Ð³Ð° Ð² Ð»ÑÑÐ¸ÑÑÐ¾Ð¼ ÑÐ»Ð¾Ðµ Ð¿Ð¾Ð»Ñ CA1 Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð°. ÐÐÐ Ð²ÑÐ·ÑÐ²Ð°Ð»Ð¸ Ð²ÑÑÐ¾ÐºÐ¾ÑÐ°ÑÑÐ¾ÑÐ½Ð¾Ð¹ ÑÑÐ¸Ð¼ÑÐ»ÑÑÐ¸ÐµÐ¹ (ÐÐ§Ð¡). ÐÑÑÑÐ½Ð¸Ð»Ð¾ÑÑ, ÑÑÐ¾ ÑÐ¶Ðµ Ð½Ð° 3 Ð¸ 7 Ð´ÐµÐ½Ñ Ð¿Ð¾ÑÐ»Ðµ Ð¿ÐµÑÐµÐ½ÐµÑÐµÐ½Ð½Ð¾Ð³Ð¾ Ð­Ð¡ Ð½Ð°Ð±Ð»ÑÐ´Ð°ÐµÑÑÑ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ðµ ÑÑÐ¾Ð²Ð½Ñ ÐÐÐ. ÐÐ° 3 ÑÑÑÐºÐ¸ Ð±ÑÐ» Ð²ÑÑÐ²Ð»ÐµÐ½ NMDA-R Ð½ÐµÐ·Ð°Ð²Ð¸ÑÐ¸Ð¼ÑÐ¹ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼ Ð¸Ð½Ð´ÑÐºÑÐ¸Ð¸ ÐÐÐ. Ð£ ÐºÑÑÑ ÑÐµÑÐµÐ· 1 Ð´ÐµÐ½Ñ Ð¿Ð¾ÑÐ»Ðµ Ð­Ð¡ ÐÐÐ Ð¿Ð¾Ð»Ð½Ð¾ÑÑÑÑ Ð¾Ð±ÑÑÐ»Ð¾Ð²Ð»ÐµÐ½Ð° ÑÐ°Ð±Ð¾ÑÐ¾Ð¹ GluN2B-ÑÐ¾Ð´ÐµÑÐ¶Ð°ÑÐ¸Ñ NMDA-ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð², Ð° Ð·Ð°ÑÐµÐ¼ Ð² ÑÐµÑÐµÐ½Ð¸Ðµ Ð¿ÐµÑÐ²Ð¾Ð¹ Ð½ÐµÐ´ÐµÐ»Ð¸ Ð¿ÑÐ¾Ð¸ÑÑÐ¾Ð´Ð¸Ñ Ð¿ÐµÑÐµÑÑÑÐ¾Ð¹ÐºÐ° ÑÑÐ±ÑÐµÐ´Ð¸Ð½Ð¸ÑÐ½Ð¾Ð³Ð¾ ÑÐ¾ÑÑÐ°Ð²Ð° NMDA-ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð². ÐÐ»Ð¸Ð°Ð»ÑÐ½ÑÐ¹ Ð½ÐµÐ¹ÑÐ¾ÑÑÐ°Ð½ÑÐ¼Ð¸ÑÑÐµÑ D-ÑÐµÑÐ¸Ð½ ÑÑÐ¸Ð»Ð¸Ð²Ð°Ð» ÐÐÐ Ð½Ð° 7-Ðµ ÑÑÑÐºÐ¸ Ð¿Ð¾ÑÐ»Ðµ Ð­Ð¡, ÑÑÐ¾ Ð¼Ð¾Ð¶ÐµÑ ÑÐ²Ð¸Ð´ÐµÑÐµÐ»ÑÑÑÐ²Ð¾Ð²Ð°ÑÑ Ð¾ Ð½Ð°ÑÑÑÐµÐ½Ð¸Ð¸ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð¾Ð² Ð½ÐµÐ¹ÑÐ¾Ð½-Ð°ÑÑÑÐ¾ÑÐ¸ÑÐ°ÑÐ½ÑÑ Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ð¹ Ð² Ð¿Ð¾Ð´Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ð¸ Ð¿Ð»Ð°ÑÑÐ¸ÑÐ½Ð¾ÑÑÐ¸ Ð¿Ð¾ÑÐ»Ðµ Ð­Ð¡. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ Ð´Ð°Ð½Ð½ÑÐµ Ð¾ÑÑÐ°Ð¶Ð°ÑÑ Ð½ÐµÐ³Ð°ÑÐ¸Ð²Ð½Ð¾Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð¿Ð¸Ð»Ð¾ÐºÐ°ÑÐ¿Ð¸Ð½-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð­Ð¡ Ð½Ð° ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÐÐÐ. Ð Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐµÐ¼ Ð´Ð°Ð½Ð½Ð¾Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¼Ð¾Ð¶ÐµÑ Ð¿Ð¾Ð¼Ð¾ÑÑ Ð½Ð°Ð¹ÑÐ¸ Ð½Ð¾Ð²ÑÐµ Ð¼ÐµÑÐ¾Ð´Ñ Ð»ÐµÑÐµÐ½Ð¸Ñ Ð¸ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐ¸ ÐÐ­.
description: We have conducted a number of electrophysiological studies confirming that the convulsive States suffered significantly affect the characteristics of synaptic plasticity in the CA1 field of the rat hippocampus. The purpose of this work was to study the dynamics and mechanisms of formation of long-term synaptic potentiation (LTP) in the CA1 field of the rat hippocampus in the latent phase of the lithium-pilocarpine model of temporal epilepsy (TE) in rats. Studies were conducted 1, 3, 7 days after pilocarpine-induced epileptic status (SE). Registration of extra-cellular field excitatory postsynaptic potentials (fEPSPs) was performed on experiencing brain sections in the radiant layer of the hippocampal CA1 field. LTP was caused by high-frequency stimulation (HFS). It turned out that already on the 3rd and 7th day after the SE, there is a decrease in the level of LTP. On 3rd day, was detected NMDA-R independent mechanism of LTP induction. On 1st day after SE LTP is completely depends on the work of GluN2B-containing NMDA receptors, and then during the first week there is a restructuring of the subunit composition of NMDA receptors. Glial neurotransmitter D-serine increased LTP on the 7th day after SE, which may indicate a violation of the mechanisms of neuron-astrocyte relations in maintaining plasticity after SE. The obtained data reflect the negative impact of pilocarpine-induced SE on the formation of LTP. In the future, this study may help to find new methods of treatment and diagnosis of temporal lobe epilepsy.