Differential proteomics analysis of Frankliniella occidentalis immune response after infection with Tomato spotted wilt virus (Tospovirus)
Creators: name: Ogada, Pamella Akoth
name: Kiirika, Leonard Muriithi
name: Lorenz, Christin
name: Senkler, Jennifer
name: Braun, Hans-Peter
name: Poehling, Hans-Michael
Publisher: name: Amsterdam [u.a.] : Elsevier Science
Year: 2017
Subjects: subject: 2D-IEF/SDS/PAGE
subject: Frankliniella occidentalis
subject: Innate immunity
subject: Mass spectrometry
subject: Proteomics
subject: Tomato spotted wilt virus
subject: actin
subject: actin related protein 2
subject: adenosylhomocysteinase
subject: aldehyde reductase
subject: aldose reductase related protein 1
subject: alpha tubulin
subject: cathepsin L
subject: chitinase
subject: cyclic AMP dependent protein kinase anchoring protein
subject: dermcidin
subject: DNA polymerase Rev1
subject: enolase
subject: fibrinogen
subject: guanine nucleotide binding protein beta subunit
subject: guanosine phosphate reductase
subject: heat shock protein 70
subject: heterochromatin protein 1
subject: hornerin
subject: hyaluronidase
subject: inactive hyaluronidase B
subject: initiation factor 3
subject: initiation factor 5A
subject: khellin
subject: membrane protein
subject: proton transporting adenosine triphosphate synthase
subject: ribosome protein
subject: ribosome protein L12
subject: ubiquitin
subject: unclassified drug
subject: unindexed drug
subject: valosin containing interacting protein 135
subject: insect protein
subject: peptide
subject: transcriptome
subject: Article
subject: immune response
subject: innate immunity
subject: isoelectric focusing
subject: mass spectrometry
subject: nonhuman
subject: nucleotide metabolism
subject: pilot study
subject: polyacrylamide gel electrophoresis
subject: priority journal
subject: protein analysis
subject: protein expression
subject: protein function
subject: proteomics
subject: virus isolation
subject: animal
subject: arthropod vector
subject: gene expression regulation
subject: genetics
subject: immunology
subject: larva
subject: lipid metabolism
subject: metabolism
subject: Thysanoptera
subject: Tospovirus
subject: Animals
subject: Arthropod Vectors
subject: Gene Expression Regulation
subject: HSP70 Heat-Shock Proteins
subject: Immunity, Innate
subject: Insect Proteins
subject: Larva
subject: Lipid Metabolism
subject: Mass Spectrometry
subject: Peptides
subject: Transcriptome
subject: Ubiquitin
subject: Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit

description: Tomato spotted wilt virus (TSWV) is mainly vectored by Frankliniella occidentalis Pergande, and it potentially activates the vector's immune response. However, molecular background of the altered immune response is not clearly understood. Therefore, using a proteomic approach, we investigated the immune pathways that are activated in F. occidentalis larvae after 24 h exposure to TSWV. Two-dimensional isoelectric focusing/sodium dodecyl sulfate polyacrylamide gel electrophoresis (2D-IEF/SDS/PAGE) combined with mass spectrometry (MS), were used to identify proteins that were differentially expressed upon viral infection. High numbers of proteins were abundantly expressed in F. occidentalis exposed to TSWV (73%) compared to the non-exposed (27%), with the majority functionally linked to the innate immune system such as: signaling, stress response, defense response, translation, cellular lipids and nucleotide metabolism. Key proteins included: 70 kDa heat shock proteins, Ubiquitin and Dermcidin, among others, indicative of a responsive pattern of the vector's innate immune system to viral infection. © 2016 Elsevier Ltd