title: ÐÐµÑÐ¾Ð´ Ð±ÑÑÑÑÐ¾Ð¹ ÑÐ¸ÐºÐ»Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð²Ð¾Ð»ÑÑÐ°Ð¼Ð¿ÐµÑÐ¾Ð¼ÐµÑÑÐ¸Ð¸ Ð´Ð»Ñ Ð¿ÑÐ¸Ð¶Ð¸Ð·Ð½ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¸Ð·Ð¼ÐµÑÐµÐ½Ð¸Ñ ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ñ Ð´Ð¾ÑÐ°Ð¼Ð¸Ð½Ð° Ð² ÑÐµÐ¶Ð¸Ð¼Ðµ ÑÐµÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð²ÑÐµÐ¼ÐµÐ½Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: A method of fastÂ scanning cyclic voltammetry for lifetime measurement of dopamine content in real time
Creators: name: ÐÑÑÑÐºÐ¾Ð², ÐÐ¼Ð¸ÑÑÐ¸Ð¹
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: ÐÐ¡Ð¦ÐÐ
Ð´Ð¾ÑÐ°Ð¼Ð¸Ð½
Ð±ÑÑÑÑÐ¾ ÑÐºÐ°Ð½Ð¸ÑÑÑÑÐ°Ñ ÑÐ¸ÐºÐ»Ð¸ÑÐµÑÐºÐ°Ñ Ð²Ð¾Ð»ÑÑÐ°Ð¼Ð¿ÐµÑÐ¾Ð¼ÐµÑÑÐ¸Ñ
FSCV
dopamine
fast-scan ciclic voltammetry

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·Ð¼ÐµÑÐµÐ½Ð¸Ñ Ð´Ð¾ÑÐ°Ð¼Ð¸Ð½Ð° Ñ ÑÐ²Ð¾Ð±Ð¾Ð´Ð½Ð¾ Ð¿ÐµÑÐµÐ¼ÐµÑÐ°ÑÑÐ¸ÑÑÑ ÐºÑÑÑ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¼ÐµÑÐ¾Ð´Ñ Ð±ÑÑÑÑÐ¾Ð¹ ÑÐ¸ÐºÐ»Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð²Ð¾Ð»ÑÑÐ°Ð¼Ð¿ÐµÑÐ¾Ð¼ÐµÑÑÐ¸Ð¸. ÐÐ¾ÑÐ°Ð¼Ð¸Ð½ - ÐºÐ»ÑÑÐµÐ²Ð¾Ð¹ Ð¼Ð¾Ð½Ð¾Ð°Ð¼Ð¸Ð½, ÑÑÐ°ÑÑÐ²ÑÑÑÐ¸Ð¹ Ð² Ð²Ð¾Ð·Ð½Ð°Ð³ÑÐ°Ð¶Ð´ÐµÐ½Ð¸Ð¸, Ð¾Ð±ÑÑÐµÐ½Ð¸Ð¸, Ð¿Ð°Ð¼ÑÑÐ¸, Ð°Ð³ÑÐµÑÑÐ¸Ð¸ Ð¸ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ Ð´ÑÑÐ³Ð¸Ñ ÑÑÐ½ÐºÑÐ¸ÑÑ. Ð¡Ð»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾, Ð½Ð°ÑÑÑÐµÐ½Ð¸Ðµ ÑÐµÐ³ÑÐ»ÑÑÐ¸Ð¸ Ð´Ð¾ÑÐ°Ð¼Ð¸Ð½ÐµÑÐ³Ð¸ÑÐµÑÐºÐ¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ñ Ð¼Ð¾Ð¶ÐµÑ Ð¿ÑÐ¸Ð²ÐµÑÑÐ¸ Ðº ÑÐµÑÑÐµÐ·Ð½ÑÐ¼ Ð½ÐµÐ³Ð°ÑÐ¸Ð²Ð½ÑÐ¼ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÐ²Ð¸ÑÐ¼ Ð²Ð¾ Ð¼Ð½Ð¾Ð³Ð¸Ñ Ð°ÑÐ¿ÐµÐºÑÐ°Ñ ÑÐµÐ»Ð¾Ð²ÐµÑÐµÑÐºÐ¾Ð¹ Ð¶Ð¸Ð·Ð½Ð¸. ÐÐ»Ñ Ð±Ð¾Ð»ÐµÐµ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾Ð¹ ÐºÐ¾ÑÑÐµÐºÑÐ¸Ð¸ Ð¿Ð°ÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð´Ð¾ÑÐ°Ð¼Ð¸Ð½ÐµÑÐ³Ð¸ÑÐµÑÐºÐ¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ñ ÐºÑÐ°Ð¹Ð½Ðµ Ð²Ð°Ð¶Ð½Ð¾ Ð¸Ð¼ÐµÑÑ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÐµÐ½Ð¸Ðµ Ð¾Ð± ÑÑÐ¾Ð²Ð½Ðµ Ð´Ð¾ÑÐ°Ð¼Ð¸Ð½Ð° Ð² ÑÐµÑÐµÐ½Ð¸Ðµ Ð´Ð½Ñ. ÐÐµÑÐ¾Ð´ ÑÐ¸ÐºÐ»Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð²Ð¾Ð»ÑÑÐ°Ð¼Ð¿ÐµÑÐ¾Ð¼ÐµÑÑÐ¸Ð¸ Ñ Ð±ÑÑÑÑÑÐ¼ ÑÐºÐ°Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÐµÑ Ð¿Ð¾Ð»ÑÑÐ¸ÑÑ Ð²Ð¾Ð»ÑÑÐ°Ð¼Ð¿ÐµÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ñ, ÐºÐ¾ÑÐ¾ÑÐ°Ñ Ð²Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÐ²Ð¸Ð¸ Ð¼Ð¾Ð¶ÐµÑ Ð±ÑÑÑ Ð¿ÑÐµÐ¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð° Ð² ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ñ Ð´Ð¾ÑÐ°Ð¼Ð¸Ð½Ð° Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð²Ð½ÐµÑÐ½Ð¸Ñ ÑÑÐ°Ð½Ð´Ð°ÑÑÐ¾Ð². Ð¦ÐµÐ»Ñ ÑÐ°Ð±Ð¾ÑÑ: ÐÐ¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°ÑÑÂ  Ð¼ÐµÑÐ¾Ð´ ÐÐ¡Ð¦ÐÐ Ð´Ð»Ñ Ð¿ÑÐ¸Ð¶Ð¸Ð·Ð½ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¸Ð·Ð¼ÐµÑÐµÐ½Ð¸Ñ Ð´Ð¾ÑÐ°Ð¼Ð¸Ð½Ð° Ñ ÑÐ²Ð¾Ð±Ð¾Ð´Ð½Ð¾ Ð¿ÐµÑÐµÐ¼ÐµÑÐ°ÑÑÐµÐ³Ð¾ÑÑ Ð¶Ð¸Ð²Ð¾ÑÐ½Ð¾Ð³Ð¾. ÐÐ°Ð´Ð°ÑÐ¸: 1. ÐÐ¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°ÑÑ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ñ Ð´Ð»Ñ ÐÐ¡Ð¦ÐÐ Ð´Ð»Ñ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð½Ð° ÑÐ²Ð¾Ð±Ð¾Ð´Ð½Ð¾ Ð¿ÐµÑÐµÐ¼ÐµÑÐ°ÑÑÐ¸ÑÑÑ Ð¶Ð¸Ð²Ð¾ÑÐ½ÑÑ. 2 ÐÐ¿ÑÐ¾Ð±Ð¸ÑÐ¾Ð²Ð°ÑÑ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ñ Ð½Ð° Ð¸Ð½ÑÐ°ÐºÑÐ½ÑÑ Ð¶Ð¸Ð²Ð¾ÑÐ½ÑÑ Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ ÑÑÐ°Ð½Ð´Ð°ÑÑÐ½Ð¾Ð¹ ÑÐ»ÐµÐºÑÑÐ¸ÑÐµÑÐºÐ¾Ð¹ ÑÑÐ¸Ð¼ÑÐ»ÑÑÐ¸Ð¸. Ð Ð°Ð±Ð¾ÑÐ° Ð±ÑÐ»Ð° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð² ÐÐ°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ Ð½ÐµÐ¹ÑÐ¾ÑÐ¸Ð¼Ð¸Ð¸ Ð¤Ð¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¾ÑÐ´ÐµÐ»Ð° Ð¸Ð¼. Ð.Ð. ÐÐ°Ð²Ð»Ð¾Ð²Ð° Ð¤ÐÐÐÐ£ ÐÐ­Ð. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð¿ÑÐ¾Ð´ÐµÐ»Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ Ð² Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ Ð±ÑÐ» ÑÐ¿ÑÐ¾ÐµÐºÑÐ¸ÑÐ¾Ð²Ð°Ð½ Ð¿ÑÐ¸Ð±Ð¾Ñ Ð¢ÐµÐ»Ðµ-ÐÐ¾Ð»ÑÑÐÐ¼Ð¿ÐµÑÐ¼ÐµÑÑ, Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÑÑÐ¸Ð¹ Ð¸Ð·Ð¼ÐµÑÑÑÑ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ñ Ð´Ð¾ÑÐ°Ð¼Ð¸Ð½Ð° Ð² ÑÑÐ±ÑÐµÐºÑÐ½Ð´Ð½Ð¾Ð¼ Ð¼Ð°ÑÑÑÐ°Ð±Ðµ Ñ ÑÐ²Ð¾Ð±Ð¾Ð´Ð½Ð¾ Ð¿ÐµÑÐµÐ¼ÐµÑÐ°ÑÑÐ¸ÑÑÑ Ð¶Ð¸Ð²Ð¾ÑÐ½ÑÑ.
description: This work is devoted to the measurement of dopamine in freely moving rats using the methods of fast cyclic voltammetry. Dopamine is a key monoamine involved in reward, learning, memory, aggression, and various other functions. Consequently, a violation of the regulation of the dopaminergic system can lead to serious negative consequences in many aspects of human life. For a more effective correction of the pathology of the dopaminergic system, it is extremely important to have an idea of the level of dopamine during the day. The method of cyclic voltammetry with fast scanning allows you to obtain a voltammogram, which can subsequently be converted into a dopamine concentration using external standards. The purpose of the work: To modify the FSCV method for lifetime measurement of dopamine in a freely moving animal. Tasks: 1. Modify the design for the FSCV for use on freely moving animals. 2 To test the modified design on intact animals under the conditions of standard electrical stimulation. The work was performed in the Laboratory of Neurochemistry of the I.P. Pavlov Physiological Department of the IEM FSBI. As a result of the work done, a Tele-voltammeter device was designed in the laboratory, which allows measuring the concentration of dopamine on a subsecond scale in freely moving animals.