Additional file 1 of Novel approach to enhance Bradyrhizobium diazoefficiens nodulation through continuous induction of ROS by manganese ferrite nanomaterials in soybean
Creators: name: Ma, Jun
name: Zhou, Yi
name: Li, Jiaying
name: Song, Zhiyong
name: Han, Heyou
Publisher: name: figshare
Year: 2022
Subjects: subject: Plant Biology
subject: FOS: Biological sciences

description: Additional file 1: Fig. S1. TEM images of (a) MF-NPs, (b) M-NPs, and (c) F-NPs. Fig. S2. Fourier transform infrared (FTIR) spectra of F-NPs, M-NPs and MF-NPs. Fig. S3. Comparison of the efficiency of oxygen production by different materials. Fig.S4. Ultraviolet scan curve of MB fading at different reaction time points in the presence of NPs and CMs. Fig. S5. Aboveground phenotypic images of soybean plants treated with different NPs. Fig. S6. Soybean plant height under exposure to different materials. *P &lt; 0.05, **P &lt; 0.01, and ***P &lt; 0.001 respectively indicate 3 different significance levels. Fig. S7. Stem diameter of soybean plant under exposure to different materials. *P &lt; 0.05, **P &lt; 0.01, and ***P &lt; 0.001 respectively indicate three different significance levels. Fig. S8. Phenotypic image of root system of soybean treated with different NPs. Fig. S9. Root length of soybean treated with different NPs. *P &lt; 0.05, **P &lt; 0.01, and ***P &lt; 0.001 respectively indicate three different significance levels. Fig. S10. Total biomass of soybean under exposure to different NPs. *P &lt; 0.05, **P &lt; 0.01, and ***P &lt; 0.001 respectively indicate three different significance levels. Fig. S11. Number of soybean nodules under exposure to different NPs. *P &lt; 0.05, **P &lt; 0.01, and ***P &lt; 0.001 respectively indicate three different significance levels. Fig. S12. Weight of soybean nodules under exposure to different NPs. *P &lt; 0.05, **P &lt; 0.01, and ***P &lt; 0.001 respectively indicate three different significance levels. Fig.S13. Quantitative analysis of total ROS in root. *P &lt; 0.05, **P &lt; 0.01, and ***P &lt; 0.001 respectively indicate three different significance levels. Fig. S14. Number of differentially expressed genes (DEGs) detected in the three stages (early, middle, and late) post inoculation. Red indicates the significantly up-regulated DEGs. Green indicates the significantly down-regulated DEGs. Blue indicates DEGs with no significant expression change. Fig. S15. Heat map of cluster analysis of differentially expressed genes in the three different stages post inoculation. Fig. S16. GO enrichment histogram of differentially expressed genes (DEGs, early stage). Fig. S17. GO enrichment histogram of differentially expressed genes (DRGs, middle stage). Fig. S18. Directed acyclic graph (DAG) of enriched GO terms (late stage). Fig. S19. Directed acyclic graph (DAG) of GO enrichment analysis of ROS metabolism-related genes (late stage). Fig. S20. KEGG pathway of biosynthesis of various secondary metabolites—part 2 in response to MF-NPs treatment. Fig. S21. KEGG pathway of flavonoid biosynthesis in response to MF-NPs treatment. Fig. S22. KEGG pathway of glutathione metabolism in response to MF-NPs treatment. Fig. S23. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway of nitrogen metabolism in response to MF-NPs treatment. Fig. S24. Standard curve of nitrogenase activity. Table S1. Elemental content of NPs. Table S2. Expression of nodulation related (Nod-R) genes in the roots in response to MF-NPs treatment. Table S3. Expression of flavonoid related (Fla-R) genes in the roots in response to MF-NPs treatment. Table S4. Expression of reactive oxygen species related (ROS-R) genes in roots in response to MF-NPs treatment.