title: ÐÐ·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ðµ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ¾Ð² ÑÐ¸Ð±ÑÐ¸Ð»Ð»Ð¾Ð³ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð° Ñ ÐÐÐ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Interaction of complexes of fibrillogenic peptide with DNA
Creators: name: ÐÑÐ·Ð°Ð¼Ð°ÑÑÐµÐ², ÐÑÐ¸Ð³Ð¾ÑÐ¸Ð¹
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: ÐÐµÐ¿ÑÐ¸Ð´Ñ
ÐÑÐºÐ»ÐµÐ¸Ð½Ð¾Ð²ÑÐµ ÐºÐ¸ÑÐ»Ð¾ÑÑ
Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð¾Ð¿Ð¾Ð´Ð¾Ð±Ð½ÑÐµ ÑÐ¸Ð±ÑÐ¸Ð»Ð»Ñ
Ð¼ÐµÐ¶Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾Ðµ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ðµ
Ð´Ð²ÑÑ ÑÐµÐ¿Ð¾ÑÐµÑÐ½Ð°Ñ ÐÐÐ
Ð·ÐµÑÐºÐ°Ð»ÑÐ½Ð¾-ÑÐ¸Ð¼Ð¼ÐµÑÑÐ¸ÑÐ½ÑÐµ Ð¼Ð¾ÑÐ¸Ð²Ñ
Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð°Ñ Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐ°

description: Ð ÑÐ°Ð±Ð¾ÑÐµ Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸ÑÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ¾Ð² ÑÐ¸Ð±ÑÐ¸Ð»Ð»Ð¾Ð³ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð° SID Ñ Ð¼Ð¾Ð´ÐµÐ»ÑÐ½ÑÐ¼Ð¸ Ð´Ð²ÑÑÑÐµÐ¿Ð¾ÑÐµÑÐ½ÑÐ¼Ð¸ ÐÐÐ ÑÐ°Ð·Ð»Ð¸ÑÐ½Ð¾Ð¹ Ð´Ð»Ð¸Ð½Ñ Ð¸ Ð½ÑÐºÐ»ÐµÐ¾ÑÐ¸Ð´Ð½Ð¾Ð³Ð¾ ÑÐ¾ÑÑÐ°Ð²Ð°. Ð¦ÐµÐ»ÑÑ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¾ Ð¾ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸Ð·Ð¾Ð²Ð°ÑÑ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ ÑÐ²ÑÐ·ÑÐ²Ð°Ð½Ð¸Ñ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð° Ñ Ð´ÑÐÐÐ, Ð¾Ð¿Ð¸ÑÐ°ÑÑ ÑÐ¿ÐµÑÐ¸ÑÐ¸ÐºÑ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ð¼Ð¾Ð»ÐµÐºÑÐ», Ð¿ÑÐ¾Ð°Ð½Ð°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°ÑÑ ÐºÐ¾Ð½ÑÐ¾ÑÐ¼Ð°ÑÐ¸Ð¾Ð½Ð½ÑÐµ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ ÐÐÐ Ð¿ÑÐ¸ ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ¾Ð². ÐÐµÑÐ¾Ð´Ð°Ð¼Ð¸ ÑÐµÑÐ°ÑÐ´Ð°ÑÐ¸Ð¸ Ð² Ð°Ð³Ð°ÑÐ¾Ð·Ð½Ð¾Ð¼ Ð³ÐµÐ»Ðµ Ð¸ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð¼Ð¸ÐºÑÐ¾Ð¼Ð°ÑÑÑÐ°Ð±Ð½Ð¾Ð³Ð¾ ÑÐµÑÐ¼Ð¾ÑÐ¾ÑÐµÐ·Ð° Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð²ÑÐ±ÑÐ°Ð½Ð½ÑÐ¹ Ð¿ÐµÐ¿ÑÐ¸Ð´ ÑÐ¿Ð¾ÑÐ¾Ð±ÐµÐ½ Ðº Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ñ Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð¾Ð¿Ð¾Ð´Ð¾Ð±Ð½ÑÑ ÑÐ¸Ð±ÑÐ¸Ð»Ð» Ð¸ Ðº ÑÐ²ÑÐ·ÑÐ²Ð°Ð½Ð¸Ñ Ð¼Ð¾Ð´ÐµÐ»ÑÐ½ÑÑ ÐÐÐ Ð² ÑÐ°ÑÑÐ²Ð¾ÑÐµ. ÐÑÐ´Ð²Ð¸Ð½ÑÑÐ° Ð¸ Ð¾Ð¿Ð¸ÑÐ°Ð½Ð° Ð³Ð¸Ð¿Ð¾ÑÐµÐ·Ð° Ð¾ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð°Ñ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ¾Ð² Ð¿ÐµÐ¿ÑÐ¸Ð´Ð° SID Ñ ÐÐÐ. ÐÐµÑÐ¾Ð´Ð°Ð¼Ð¸ Ð´Ð¸Ð½Ð°Ð¼Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐ°ÑÑÐµÑÐ½Ð¸Ñ ÑÐ²ÐµÑÐ°, Ð°Ð½Ð°Ð»Ð¸Ð·Ð° ÑÑÐ°ÐµÐºÑÐ¾ÑÐ¸Ð¹ Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸Ñ, Ð¼Ð°Ð»Ð¾ÑÐ³Ð»Ð¾Ð²Ð¾Ð³Ð¾ ÑÐ°ÑÑÐµÑÐ½Ð¸Ñ Ð½ÐµÐ¹ÑÑÐ¾Ð½Ð¾Ð² Ð¸ Ð¿ÑÐ¾ÑÐ²ÐµÑÐ¸Ð²Ð°ÑÑÐµÐ¹ ÑÐ»ÐµÐºÑÑÐ¾Ð½Ð½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸ Ð¸Ð·ÑÑÐµÐ½Ð° Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ñ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ¾Ð² Ð¿ÐµÐ¿ÑÐ¸Ð´-ÐÐÐ. ÐÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð°Ñ Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐ° Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ð¼Ð¾Ð´ÐµÐ»ÑÐ½Ð¾Ð¹ Ð´ÑÐÐÐ Ñ Ð²ÑÐ±ÑÐ°Ð½Ð½ÑÐ¼ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð¼. Ð ÐµÐ·ÑÐ»ÑÑÐ°ÑÑ ÑÐ°Ð±Ð¾ÑÑ Ð¼Ð¾Ð³ÑÑ Ð±ÑÑÑ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ñ Ð² ÑÐ°ÑÐ¼Ð°ÐºÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¿ÑÐ¸ ÑÐ¾Ð·Ð´Ð°Ð½Ð¸Ð¸ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð½ÑÑ Ð°Ð³ÐµÐ½ÑÐ¾Ð², Ð½Ð°ÑÐµÐ»ÐµÐ½Ð½ÑÑ Ð½Ð° Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ðµ Ñ ÐÐÐ, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¿ÑÐ¸ ÑÑÐ½Ð´Ð°Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸ÑÑ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð¾Ð² ÑÐ²ÑÐ·ÑÐ²Ð°Ð½Ð¸Ñ ÐÐÐ Ð¸ Ð±ÐµÐ»ÐºÐ¾Ð².
description: This thesis provides information about the interaction between the fibrilogenic peptide SID with some double-stranded DNA models of various lengths and nucleotide sequences. The aim of the work was to characterize the ability of the peptide to bind to the dsDNA, to describe the specifics of this interaction, and to analyze the conformational changes in the DNA during the complex formation. Retardation in agarose gel and microscale thermophoresis shown that the selected peptide is capable of forming amyloid-like fibrils and binds model DNA in solution. A hypothesis on the interaction mechanisms of the complexes of the SID peptide with DNA was described. The morphology of the peptide-DNA complexes was characterized using dynamic light scattering, nanotracking analysis, small-angle neutron scattering, and transmission electron microscopy. Molecular dynamics of the interaction of the double-stranded DNA with the selected peptide was performed. The research results can be applied in creating peptide drugs that have the DNA as their target. Also, the results would be useful for future theoretical studies in this area.