title: ÐÑÐ¿ÑÑÐ°Ð½Ð¸Ñ ÑÐ¸ÑÑÐµÐ¼Ñ Ð´Ð»Ñ ÐÐ¦Ð -Ð Ð Ð² ÑÐµÐ»ÑÑ ÑÐ¾Ð·Ð´Ð°Ð½Ð¸Ñ ÑÐ°Ð±Ð¾ÑÐµÐ³Ð¾ ÑÑÐ°Ð»Ð¾Ð½Ð°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Testing the real-time PCR system to create a working standard
Creators: name: ÐÐ¸ÑÐ½Ð¸ÑÑÐ½Ð°, ÐÐ½Ð°ÑÑÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: ÐÐ¼Ð¼ÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ñ
Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð½Ð°Ñ ÑÐµÐ¿Ð½Ð°Ñ ÑÐµÐ°ÐºÑÐ¸Ñ (Ð¿ÑÑ)
Ð¼ÐµÑÐ¾Ð´ ÑÐ¸ÑÑÐ¾Ð²Ð¾Ð¹ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð½Ð¾Ð¹ ÑÐµÐ¿Ð½Ð¾Ð¹ ÑÐµÐ°ÐºÑÐ¸Ð¸ (ÑÐ¿ÑÑ)
Ð¼ÐµÑÐ¾Ð´ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð½Ð¾Ð¹ ÑÐµÐ¿Ð½Ð¾Ð¹ ÑÐµÐ°ÐºÑÐ¸Ð¸ Ð² ÑÐµÐ°Ð»ÑÐ½Ð¾Ð¼ Ð²ÑÐµÐ¼ÐµÐ½Ð¸ (Ð¿ÑÑ-ÑÐ²)
ÑÐ°Ð±Ð¾ÑÐ¸Ð¹ ÑÑÐ°Ð»Ð¾Ð½

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÐ¿ÑÑÐ°Ð½Ð¸ÑÐ¼ Ð¿ÑÐ¸Ð±Ð¾ÑÐ° Ð´Ð»Ñ ÐÐ¦Ð -Ð Ð, Ð° Ð¸Ð¼ÐµÐ½Ð½Ð¾ Ð°Ð¼Ð¿Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¾ÑÐ° Ð½ÑÐºÐ»ÐµÐ¸Ð½Ð¾Ð²ÑÑ ÐºÐ¸ÑÐ»Ð¾Ñ CFX96, Ð² ÑÐµÐ»ÑÑ ÑÐ¾Ð·Ð´Ð°Ð½Ð¸Ñ ÑÐ°Ð±Ð¾ÑÐµÐ³Ð¾ ÑÑÐ°Ð»Ð¾Ð½Ð° Ð´Ð»Ñ Ð¿ÐµÑÐµÐ´Ð°ÑÐ¸ ÐµÐ´Ð¸Ð½Ð¸ÑÑ Ð²ÐµÐ»Ð¸ÑÐ¸Ð½Ñ - Ñ Ð±Ð¾Ð»ÐµÐµ Ð²ÑÑÐ¾ÐºÐ¾Ð³Ð¾ Ð¸ÐµÑÐ°ÑÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÑÐ¾Ð²Ð½Ñ ÑÐ°Ð±Ð¾ÑÐ¸Ð¼ ÑÑÐµÐ´ÑÑÐ²Ð°Ð¼ Ð¸Ð·Ð¼ÐµÑÐµÐ½Ð¸Ð¹, Ð¸ÑÐ¿Ð¾Ð»ÑÐ·ÑÐµÐ¼ÑÐ¼ Ð² ÐºÐ»Ð¸Ð½Ð¸ÑÐµÑÐºÐ¸Ñ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸ÑÑ. Ð ÑÐ¾Ð´Ðµ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð½ÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ ÑÐµÑÐ°Ð»Ð¸ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð·Ð°Ð´Ð°ÑÐ¸: 1. ÐÑÐ±Ð¾Ñ Ð¸ Ð¾Ð¿Ð¸ÑÐ°Ð½Ð¸Ðµ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ð° ÑÑÐ°Ð½Ð´Ð°ÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±ÑÐ°Ð·ÑÐ° (Ð¡Ð), Ð¿Ð¾Ð´Ð±Ð¾Ñ Ð¿ÑÐ°Ð¹Ð¼ÐµÑÐ¾Ð² Ð¸ Ð·Ð¾Ð½Ð´Ð¾Ð² Ð´Ð»Ñ Ð°Ð¼Ð¿Ð»Ð¸ÑÐ¸ÑÐ¸ÑÑÐµÐ¼ÑÑ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÐµÐ¹ Ð½ÑÐºÐ»ÐµÐ¾ÑÐ¸Ð´Ð¾Ð²; 2. ÐÑÑÐ°Ð±Ð¾ÑÐºÐ° Ð¼ÐµÑÐ¾Ð´Ð° Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¸ Ð¾ÑÐ¸ÑÑÐºÐ¸ ÐÐÐ; 3. ÐÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÐµÐ¹ Ð½ÑÐºÐ»ÐµÐ¾ÑÐ¸Ð´Ð¾Ð² Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ ÑÐ¸ÑÑÐµÐ¼ Ð´Ð»Ñ ÑÐÐ¦Ð . ÐÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð¼ÐµÑÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸Ðº Ð¡Ð. 4. ÐÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ ÑÐ¸ÑÑÐµÐ¼Ñ ÐÐ¦Ð -Ð Ð (CFX96) Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¡Ð. 5. ÐÐ±ÑÐ°Ð±Ð¾ÑÐºÐ° Ð¸ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ðµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð², Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð¼ÐµÑÐ¾Ð´Ð°Ð¼Ð¸ ÑÐÐ¦Ð  Ð¸ ÐÐ¦Ð -Ð Ð. ÐÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð¼ÐµÑÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸Ðº ÑÐ°Ð±Ð¾ÑÐµÐ³Ð¾ ÑÑÐ°Ð»Ð¾Ð½Ð°.
description: This work is devoted to testing the CFX96 nucleic acid amplifier to create a working standard to transfer unit of value from a higher hierarchical level to working measuring tools that are used in clinical laboratories. Research objectives: 1. Selection and characterization of the reference material (SRM), selection of primers and probes for specific sequences of nucleotides; 2. Extraction and purification of DNA; 3. Study of nucleotide sequences using systems of dPCR. Determination of metrological characteristics of the reference material; 4. Testing of the RT-PCR system (CFX96) using a reference material; 5. Processing and comparison of research results (dPCR and RT-PCR re-sults). Determination of the metrological characteristics of the working standard.