title: Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½ÑÑ ÑÑÐµÐ´ Ð´Ð»Ñ Ð½Ð°ÐºÐ¾Ð¿Ð»ÐµÐ½Ð¸Ñ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ð½ÑÑ Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹ ÑÐ¾Ð´Ð° Rhodotorula
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Development of nutrients for the accumulation of biomass of carotenoid yeasts of the genus Rhodotorula
Creators: name: Ð¢ÐµÐ»ÑÑ, ÐÐ°ÑÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð´ÑÐ¾Ð¶Ð¶Ð¸
Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½ÑÐµ ÑÑÐµÐ´Ñ
ÑÐ¿ÐµÐºÑÑÐ¾ÑÐ¾ÑÐ¾Ð¼ÐµÑÑÐ¸Ñ
yeast
growth medium
spectrophotometry

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐµ Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½ÑÑ ÑÑÐµÐ´ Ð´Ð»Ñ Ð½Ð°ÐºÐ¾Ð¿Ð»ÐµÐ½Ð¸Ñ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð´Ð²ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð² ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ð½ÑÑ Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹ Ð¸ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐµÐ¹ ÑÐ¿ÐµÐºÑÑÐ¾ÑÐ¾ÑÐ¾Ð¼ÐµÑÑÐ¸Ð¸ Ð¾Ð±ÑÐ°Ð·ÑÐ¾Ð² Ð´Ð»Ñ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð½Ð°ÐºÐ¾Ð¿Ð»ÐµÐ½Ð½ÑÑ ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ð¾Ð². ÐÐ°Ð´Ð°ÑÐ¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â Ð¾Ð¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ ÑÐµÑÐµÐ¿ÑÑÑÑ ÑÐ²ÐµÑÐ´ÑÑ Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½ÑÑ ÑÑÐµÐ´, Ð¿ÑÐ¾Ð²ÐµÑÑÐ¸ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹ Ð½Ð° ÑÐ²ÐµÑÐ´ÑÑ Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½ÑÑ ÑÑÐµÐ´Ð°Ñ, Ð¿ÑÐ¾Ð²ÐµÑÑÐ¸ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹ Ð½Ð° Ð¶Ð¸Ð´ÐºÐ¾Ð¹ Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ´Ðµ, Ð¿ÑÐ¾Ð²ÐµÑÑÐ¸ ÑÐ¸Ð»ÑÑÑÐ°ÑÐ¸Ñ Ð¸ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐµÐµ Ð²ÑÑÑÑÐ¸Ð²Ð°Ð½Ð¸Ðµ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹, Ð¾Ð¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ Ð¿ÐµÑÑÐ¿ÐµÐºÑÐ¸Ð²Ð½ÑÐ¹ ÑÑÐ°Ð¼Ð¼ Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹ Ñ Ð±Ð¾Ð»ÑÑÐ¸Ð¼ Ð²ÑÑÐ¾Ð´Ð¾Ð¼ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ, Ð¿ÑÐ¾Ð²ÐµÑÑÐ¸ ÑÐ¿ÐµÐºÑÑÐ¾ÑÐ¾ÑÐ¾Ð¼ÐµÑÑÐ¸Ñ Ð¾Ð±ÑÐ°Ð·ÑÐ¾Ð² Ð´Ð»Ñ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ð¾Ð². ÐÐ±ÑÐµÐºÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â Rhodotorula sp. ÑÑ. 1025, Rhodotorula roseum Ð¸Ð· ÐÑÐµÑÐ¾ÑÑÐ¸Ð¹ÑÐºÐ¾Ð¹ ÐºÐ¾Ð»Ð»ÐµÐºÑÐ¸Ð¸ ÐºÑÐ»ÑÑÑÑ ÑÐµÐ»ÑÑÐºÐ¾ÑÐ¾Ð·ÑÐ¹ÑÑÐ²ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð½Ð°Ð·Ð½Ð°ÑÐµÐ½Ð¸Ñ Ð½Ð°ÑÑÐ½Ð¾-Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÑÐºÐ¾Ð³Ð¾ Ð¸Ð½ÑÑÐ¸ÑÑÑÐ° ÑÐµÐ»ÑÑÐºÐ¾ÑÐ¾Ð·ÑÐ¹ÑÑÐ²ÐµÐ½Ð½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ð¸. ÐÐµÑÐ¾Ð´Ð°Ð¼Ð¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐ²Ð»ÑÐ»Ð¸ÑÑ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ðµ Ð¿Ð¾ÑÐµÐ²Ð½Ð¾Ð³Ð¾ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ð°, Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ðµ Ð¿Ð¾ÑÐµÐ²Ð¾Ð² 10-ÐºÑÐ°ÑÐ½ÑÑ ÑÐ°Ð·Ð²ÐµÐ´ÐµÐ½Ð¸Ð¹ Ð½Ð° ÑÐ°ÑÐºÐ¸ ÐÐµÑÑÐ¸, ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹ Ð½Ð° Ð¶Ð¸Ð´ÐºÐ¾Ð¹ Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ´Ðµ, ÑÐ¸Ð»ÑÑÑÐ°ÑÐ¸Ñ Ñ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸ÐµÐ¼ ÑÐ¸ÑÑÐ¾Ð¹ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð¸ ÑÐ¿ÐµÐºÑÑÐ¾ÑÐ¾ÑÐ¾Ð¼ÐµÑÑÐ¸Ñ. ÐÐ¾ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ°Ð¼ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ» Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½ ÑÐ¸ÑÑ Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼ÑÑ Ð¾Ð±ÑÐ°Ð·ÑÐ¾Ð², Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ñ Ð·Ð½Ð°ÑÐµÐ½Ð¸Ñ Ð¾Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÐ¸ Ð¾Ð±ÑÐ°Ð·ÑÐ¾Ð², Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½ ÑÐ°ÑÑÐµÑ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²ÐµÐ½Ð½Ð¾Ð³Ð¾ ÑÑÐ¼Ð¼Ð°ÑÐ½Ð¾Ð³Ð¾ ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ñ ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ð¾Ð².
description: This work is devoted to the development of nutrient media for the accumulation of biomass of two strains of carotenoid yeasts and subsequent spectrophotometry of samples for the processing of accumulated carotenoids. The objectives of the research are to determine the formulation of organic nutrient media, to cultivate yeast on low-calorie media, to cultivate yeast on a liquid nutrient medium, to filter and subsequently dry the yeast biomass, to determine the most promising yeast strain with a high yield of biomass, to conduct spectrophotometry of samples for the quantitative determination of carotenoids. Objects of study â Rhodotorula sp. strain 1025, Rhodotorula Roseum from the All-Russian collection of cultural agricultural purposes of the Re-search Institute of Agricultural Microbiology. The research methods included obtaining inoculum, inoculating 10-fold dilutions on Petri dishes, cultivating yeast in a liquid nutrient medium, filtration to isolate pure biomass, and spectrophotometry. Based on the results of the work, the titer of the control samples was determined, the optical density of the samples was measured, and the quantitative total content of carotenoids was calculated.