cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Additional file 4: of
                     Cryptosporidium as a testbed for single cell genome characterization of unicellular eukaryotes
Creators: name: Troell, Karin
name: Hallström, Björn
name: Anna-Maria Divne
name: Alsmark, Cecilia
name: Romanico Arrighi
name: Huss, Mikael
name: Beser, Jessica
name: Bertilsson, Stefan
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biophysics
subject: Microbiology
subject: FOS: Biological sciences
subject: Genetics
subject: Molecular Biology
subject: Biotechnology
subject: 69999 Biological Sciences not elsewhere classified
subject: 60506 Virology

description: Partial Least Squares Regression model for genome coverage based on MDA and FACS characteristics. A. Weight vector plot for predictor variables (solid squares) and response variables (open square), for the first two latest variables (LV1 + LV2). FL (antibody-derived fluorescence, FSC (forward scatter), SSC (side scatter), Ct (time to reach MDA threshold), Efficiency (efficiency of the MDA) and Yield (final DNA concentration in MDA) is used as predictor variables. B. Correlation plot based on PLS model for observed vs. predicted genome coverage. The root mean square error (RMSE) and the equation for the linear regression is displayed in the graph. Simcas (Umetrics, Umeå, Sweden) was used for the statistical model and all variables were centered at zero and normalized for variance prior to analysis. (PDF 42 kb)
Additional file 6 of Complex regulation of ADAR-mediated RNA-editing across tissues
Creators: name: Huntley, Melanie
name: Lou, Melanie
name: Goldstein, Leonard
name: Lawrence, Michael
name: Dijkgraaf, Gerrit
name: Kaminker, Joshua
name: Gentleman, Robert
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biochemistry
subject: Space Science
subject: Microbiology
subject: FOS: Biological sciences
subject: Molecular Biology
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: Developmental Biology
subject: 111714 Mental Health
subject: FOS: Health sciences
subject: 60506 Virology

description: Figure S6. Sequence motifs (produced by the motifStack R package) surrounding editing sites, separated by tissue and genic context. (PDF 2389 kb)
Additional file 1: of
                     Cryptosporidium as a testbed for single cell genome characterization of unicellular eukaryotes
Creators: name: Troell, Karin
name: Hallström, Björn
name: Anna-Maria Divne
name: Alsmark, Cecilia
name: Romanico Arrighi
name: Huss, Mikael
name: Beser, Jessica
name: Bertilsson, Stefan
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biophysics
subject: Space Science
subject: 29999 Physical Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: Microbiology
subject: FOS: Biological sciences
subject: Genetics
subject: Molecular Biology
subject: Evolutionary Biology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Cancer
subject: Hematology
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: Plant Biology
subject: 60506 Virology

description: Density profiles showing detailed view of the sequencing depth for all eight chromosomes with the 10 single cell genomes given in color according to (Fig. 2a). “All cells combined” are shown with gray shading. (PDF 222 kb)
Additional file 1: of Correlates for psycho-active substance use among boarding secondary school adolescents in Enugu, South East, Nigeria
Creators: name: Manyike, Pius
name: Josephat Chinawa
name: Awoere Chinawa
name: Obu, Herbert
name: Nwokocha, Ada R.C.
name: Odutola I. Odetunde
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biotechnology
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Computational Biology

description: WHO questionnaire, Geneva for substance use (DOCX 714Â kb)
Additional file 1: Table S1. of Assessing health-related quality of life in COPD: comparing generic and disease-specific instruments with focus on comorbidities
Creators: name: Wacker, Margarethe
name: Jörres, Rudolf
name: Karch, Annika
name: Wilke, Sarah
name: Heinrich, Joachim
name: Karrasch, Stefan
name: Koch, Armin
name: Schulz, Holger
name: Watz, Henrik
name: Leidl, Reiner
name: Vogelmeier, Claus
name: Holle, Rolf
Publisher: name: Figshare
Year: 2016
Subjects: subject: Medicine
subject: Biotechnology
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences

description: Correlations between HRQL instruments and with GOLD grade and BODE as clinical measures: Spearman Correlation Coefficients. Table S2. Adjusted mean EQ-5D utilities, EQ-5D VAS, CAT score, SGRQ total scores for COPD grade 1–4 stratified for group with low (≤3) or high (>3) number of comorbidities. Table S3. Results of regression models considering interactions between COPD grades and low/high number of comorbidity. Figure S1. Lifetime prevalence of self-reported comorbidities (%) in the study population. Figure S2. HRQL scores by FEV1 % pred.: non-parametric quantile regression: quantile fit plots for FEV1 % pred. (DOC 258 kb)
Monerista Meyrick 1925
Creators: name: Das, Apurva
name: Pathania, Prakash C.
name: Singh, Navneet
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Lepidoptera
Lecithoceridae
Monerista

description: Genus Monerista Meyrick, 1925: 208
Type species: Timyra hippastis Meyrick, 1908, by original designation
description: Published as part of Das, Apurva, Pathania, Prakash C. & Singh, Navneet, 2022, A catalogue of Indian Lecithoceridae (Lepidoptera: Gelechioidea), pp. 56-78 in Zootaxa 5165 (1) on page 72, DOI: 10.11646/zootaxa.5165.1.2, http://zenodo.org/record/6825526
Additional file 8 of Complex regulation of ADAR-mediated RNA-editing across tissues
Creators: name: Huntley, Melanie
name: Lou, Melanie
name: Goldstein, Leonard
name: Lawrence, Michael
name: Dijkgraaf, Gerrit
name: Kaminker, Joshua
name: Gentleman, Robert
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biochemistry
subject: Space Science
subject: Molecular Biology
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: Developmental Biology
subject: 60506 Virology
subject: FOS: Biological sciences

description: Figure S1. Each of the five filters was applied independently and results plotted. The final column shows the results of all five filters applied sequentially. (PDF 4 kb)
MOESM1 of Effect of the long-acting insulin analogues glargine and degludec on cardiomyocyte cell signalling and function
Creators: name: Hartmann, Thorsten
name: Overhagen, Sabrina
name: D. Ouwens
name: Raschke, Silja
name: Wohlfart, Paulus
name: Tennagels, Norbert
name: Wronkowitz, Nina
name: Eckel, Jürgen
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biophysics
subject: Biochemistry
subject: Molecular Biology
subject: Physiology
subject: FOS: Biological sciences
subject: Pharmacology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: 60506 Virology
subject: Computational Biology

description: Additional file 1: Figure S1. Competition binding assay using solubilised IR. Solubilised IR preparations were used to analyse binding of Ins (blue), IGlaM1 (green) and IDeg (red) in a competition binding assay. Percentage of binding is normalised to maximum binding of [125I]-labelled human insulin. Data represent mean values ± SEM, n = 6–7.
Additional file 3: of Genomic profiling of fungal cell wall-interfering compounds: identification of a common gene signature
Creators: name: García, Raúl
name: Botet, Javier
name: Rodríguez-Peña, José
name: Bermejo, Clara
name: Ribas, Juan
name: Revuelta, José
name: Nombela, César
name: Arroyo, Javier
Publisher: name: Figshare
Year: 2015
Subjects: subject: Biochemistry
subject: Space Science
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Molecular Biology
subject: Pharmacology
subject: Evolutionary Biology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: 60506 Virology

description: Osmotic remediation by sorbitol of ZYM, CR and CAS hypersensitivity of mutant strains belonging to the “cell wall maintenance (CWM)” group. Minimal Inhibitory concentration (MIC) assays were carried out in 96-well microtiter plates as detailed in Methods either in the absence or in the presence of 0.8 M sorbitol. (PDF 1105 kb)
Monerista hippastis Timyra
Creators: name: Das, Apurva
name: Pathania, Prakash C.
name: Singh, Navneet
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Lepidoptera
Lecithoceridae
Monerista
Monerista hippastis

description: 137. Monerista hippastis (Meyrick, 1908: 451) (Timyra)
Type locality: India, Assam, Khasi Hills [Now in Meghalaya]
Distribution: Indian records: Meghalaya.
description: Published as part of Das, Apurva, Pathania, Prakash C. & Singh, Navneet, 2022, A catalogue of Indian Lecithoceridae (Lepidoptera: Gelechioidea), pp. 56-78 in Zootaxa 5165 (1) on page 72, DOI: 10.11646/zootaxa.5165.1.2, http://zenodo.org/record/6825526
Clevelandella philipi Kotyk, Bourland, Soviš, Méndez-Sánchez, Škaloud, Varadínová & Čepička, 2024, sp.nov.
Creators: name: Kotyk, Michael
name: Bourland, William A.
name: Soviš, Matyáš
name: Méndez-Sánchez, Daniel
name: Škaloud, Pavel
name: Varadínová, Zuzana Kotyková
name: Čepička, Ivan
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Chromista
Ciliophora
Heterotrichea
Heterotrichida
Clevelandellidae
Clevelandella
Clevelandella philipi

description: Clevelandella philipi sp.nov.
ZooBank registration: urn:lsid:zoobank.org:act: 72561759- FBB1-4007-AF97-39630E48C436.
Diagnosis: Spade-shaped, dorsoventrally flattened Clevelandella with average length in protargol preparations 69 µm, mean 31 adoral membranelles, posterior protrusion of left lobe of anterior body part overhanging up to one-third of peristomial projection, and with teardrop-shaped or ellipsoidal macronucleus.
Etymology: Named in honour of Mr Philip Komong, our collaborator from Papua New Guinea.
Type locality: Papua New Guinea, Wanang 3, N5.228, E145.080.
Type host: Salganea ternatensis hirsuta Roth, 1979.
Type material: A protargol-impregnated holotype cell (Fig. 7C, D) marked in a black circle on a slide deposited in the collection of the National Museum of the Czech Republic, Prague, Czech Republic, inventory number P6E 5381.
description: Published as part of Kotyk, Michael, Bourland, William A., Soviš, Matyáš, Méndez-Sánchez, Daniel, Škaloud, Pavel, Varadínová, Zuzana Kotyková & Čepička, Ivan, 2024, Morphology maưers: congruence of morphology and phylogeny in the integrative taxonomy of Clevelandellidae (Ciliophora: Armophorea) with description of six new species, pp. 1-51 in Zoological Journal of the Linnean Society 202 (1) on page 48, DOI: 10.1093/zoolinnean/zlad154, http://zenodo.org/record/14541981
MOESM2 of Effect of the long-acting insulin analogues glargine and degludec on cardiomyocyte cell signalling and function
Creators: name: Hartmann, Thorsten
name: Overhagen, Sabrina
name: D. Ouwens
name: Raschke, Silja
name: Wohlfart, Paulus
name: Tennagels, Norbert
name: Wronkowitz, Nina
name: JĂźrgen Eckel
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biochemistry
subject: Molecular Biology
subject: Physiology
subject: FOS: Biological sciences
subject: Pharmacology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Cancer
subject: 60506 Virology
subject: Computational Biology

description: Additional file 2: Table S1. Summary of competition binding assay using S-IR.
Phatnotis Meyrick 1913
Creators: name: Das, Apurva
name: Pathania, Prakash C.
name: Singh, Navneet
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Lepidoptera
Lecithoceridae
Phatnotis

description: Genus Phatnotis Meyrick, 1913: 180
Type species: Phatnotis factiosa Meyrick, 1913, by original designation
description: Published as part of Das, Apurva, Pathania, Prakash C. & Singh, Navneet, 2022, A catalogue of Indian Lecithoceridae (Lepidoptera: Gelechioidea), pp. 56-78 in Zootaxa 5165 (1) on page 72, DOI: 10.11646/zootaxa.5165.1.2, http://zenodo.org/record/6825526
Impact of cavity shaving on residual tumor rates in patients with primary invasive carcinoma and carcinoma in situ in breast conserving surgery
Creators: name: Fernandez-Pacheco, Miriam
name: Gerken, Michael
name: Ortmann, Olaf
name: Ignatov, Atanas
name: Klinkhammer-Schalke, Monika
name: Hatzipanagiotou, Maria Eleni
name: Inwald, Elisabeth C.
Publisher: name: Universität Regensburg
Year: 2022
Subjects: subject: Breast conserving surgery · Cavity shaving · Invasive breast cancer · Re-excision rates · Residual tumor
subject: 610 Medizin

description: Background
Several international studies reported relatively high re-excision rates due to residual tumor in breast conserving surgery (BCS). Cavity shaving (CS) is a surgical strategy to reduce re-excision rates. This study aimed to investigate the effect of circumferential cavity shaving during BCS to reduce residual tumor.
Material and Methods
A total of 591 patients with early invasive carcinoma or carcinoma in situ of the breast (ICD-10, C50 or D05) who were diagnosed between 01/01/2017 and 31/12/2019 and underwent BCS in a certified breast cancer center of the University Regensburg were analyzed regarding surgical excision methods. Patients with CS during BCS and patients with targeted re-excision in a specific direction depending on the result of intraoperative mammography or sonography during BCS were compared. The risk of pathologic residual tumor (R1) was compared between both groups by means of a multivariable binary logistic regression model to determine if there is a benefit of a certain surgical method to avoid a second intervention for re-excision. We adjusted for age, tumor size, nodal status, histologic type, surgeon, breast side, and neoadjuvant chemotherapy.
Results
80 (n = 13.54%) patients had CS and 511 (n = 86.46%) had a targeted re-excision in a specific direction during BCS according to intraoperative mammography or sonography. After comparing both techniques in a multivariable regression model, there was no significant difference regarding risk of residual tumor (p = 0.738) in the total cohort. However, CS showed a tendency to be favorable regarding rates of residual tumor in patients with invasive breast cancer between 60 and 70 years (p = 0.072) and smaller T1-tumors (p = 0.057) compared to targeted intraoperative re-excision following mammographic or sonographic assessment.
Conclusion
CS showed a tendency to reduce residual tumor compared to the standard technique of intraoperative re-excision in specific subgroups, although no statistical significance was reached. Further studies are needed to overcome potential limitations like surgeon-based bias and missing standardized definitions of CS to reduce residual tumor rates.
Emblems and seraphic pedagogy: two worlds converging in the Franciscan libraries at Saint Anthony of Brazil Province (Bahia, Pernambuco and Paraíba - 18th century)
Creators: name: S. Oliveira, Carla Mary
Publisher: name: figshare
Year: 2024
Subjects: classificationCode: 430317
subject: Latin and South American history

description: Paper treating on Emblematic and FRanciscan libraries in Colonial Brazilian Northeast, 18th century.
Delay of total joint replacement is associated with a higher 90-day revision rate and increased postoperative complications
Creators: name: Holzapfel, Dominik Emanuel
name: Meyer, Matthias
name: Thieme, Max
name: Pagano, Stefano
name: von Kunow, Frederik
name: Weber, Markus
Publisher: name: Universität Regensburg
Year: 2022
Subjects: subject: Surgical delay · Surgery postponement · Elective surgery · TJR · THA · TKA · Postoperative complications · Revision rate · COVID-19 pandemic
subject: 610 Medizin

description: Purpose
Delay of elective surgeries, such as total joint replacement (TJR), is a common procedure in the current pandemic. In trauma surgery, postponement is associated with increased complication rates. This study aimed to evaluate the impact of postponement on surgical revision rates and postoperative complications after elective TJR.
Methods
In a retrospective analysis of 10,140 consecutive patients undergoing primary total hip replacement (THR) or total knee replacement (TKR) between 2011 and 2020, the effect of surgical delay on 90-day surgical revision rate, as well as internal and surgical complication rates, was investigated in a university high-volume arthroplasty center using the institute’s joint registry and data of the hospital administration. Moreover, multivariate logistic regression models were used to adjust for confounding variables.
Results
Two thousand four hundred and eighty TJRs patients were identified with a mean delay of 13.5 ± 29.6 days. Postponed TJR revealed a higher 90-day revision rate (7.1–4.5%, p < 0.001), surgical complications (3.2–1.9%, p < 0.001), internal complications (1.8–1.2% p < 0.041) and transfusion rate (2.6–1.8%, p < 0.023) than on-time TJR. Logistic regression analysis confirmed delay of TJRs as independent risk factor for 90-day revision rate [OR 1.42; 95% CI (1.18–1.72); p < 0.001] and surgical complication rates [OR 1.51; 95% CI (1.14–2.00); p = 0.04].
Conclusion
Alike trauma surgery, delay in elective primary TJR correlates with higher revision and complication rates. Therefore, scheduling should be performed under consideration of the current COVID-19 pandemic.
Level of evidence
Level III—retrospective cohort study.
The SAGA Survey. IV. The Star Formation Properties of 101 Satellite Systems around Milky Way-mass Galaxies
Creators: name: Geha, Marla
name: Mao, Yao-Yuan
name: Wechsler, Risa H.
name: Asali, Yasmeen
name: Kado-Fong, Erin
name: Kallivayalil, Nitya
name: Nadler, Ethan O.
name: Tollerud, Erik J.
name: Weiner, Benjamin
name: Reyes, Mithi A. C. de los
name: Wang, Yunchong
name: Wu, John F.
Publisher: name: arXiv
Year: 2024
Subjects: subject: Astrophysics of Galaxies (astro-ph.GA)
subject: Cosmology and Nongalactic Astrophysics (astro-ph.CO)
subject: FOS: Physical sciences

description: We present the star-forming properties of 378 satellite galaxies around 101 Milky Way analogs in the Satellites Around Galactic Analogs (SAGA) Survey, focusing on the environmental processes that suppress or quench star formation. In the SAGA stellar mass range of 10^6 to 10^10 solar masses, we present quenched fractions, star-forming rates, gas-phase metallicities, and gas content. The fraction of SAGA satellites that are quenched increases with decreasing stellar mass and shows significant system-to-system scatter. SAGA satellite quenched fractions are highest in the central 100 kpc of their hosts and decline out to the virial radius. Splitting by specific star formation rate (sSFR), the least star-forming satellite quartile follows the radial trend of the quenched population. The median sSFR of star-forming satellites increases with decreasing stellar mass and is roughly constant with projected radius. Star-forming SAGA satellites are consistent with the star formation rate--stellar mass relationship determined in the Local Volume, while the median gas-phase metallicity is higher and median HI gas mass is lower at all stellar masses. We investigate the dependence of the satellite quenched fraction on host properties. Quenched fractions are higher in systems with larger host halo mass, but this trend is only seen in the inner 100 kpc; we do not see significant trends with host color or star formation rate. Our results suggest that lower mass satellites and satellites inside 100 kpc are more efficiently quenched in a Milky Way-like environment, with these processes acting sufficiently slowly to preserve a population of star-forming satellites at all stellar masses and projected radii.
description: 25 pages, 13 figures, 2 tables. Accepted by ApJ. This paper is part of the SAGA Survey Data Release 3. Data and notebooks are available on the survey website: https://sagasurvey.org
Phatnotis factiosa Meyrick 1913
Creators: name: Das, Apurva
name: Pathania, Prakash C.
name: Singh, Navneet
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Lepidoptera
Lecithoceridae
Phatnotis
Phatnotis factiosa

description: 138. Phatnotis factiosa Meyrick, 1913: 181
Type locality: India, Tamil Nadu, Palni Hills
Distribution: Indian records: Tamil Nadu.
description: Published as part of Das, Apurva, Pathania, Prakash C. & Singh, Navneet, 2022, A catalogue of Indian Lecithoceridae (Lepidoptera: Gelechioidea), pp. 56-78 in Zootaxa 5165 (1) on page 72, DOI: 10.11646/zootaxa.5165.1.2, http://zenodo.org/record/6825526
Clevelandella sidi Kotyk, Bourland, Soviš, Méndez-Sánchez, Škaloud, Varadínová & Čepička, 2024, sp.nov.
Creators: name: Kotyk, Michael
name: Bourland, William A.
name: Soviš, Matyáš
name: Méndez-Sánchez, Daniel
name: Škaloud, Pavel
name: Varadínová, Zuzana Kotyková
name: Čepička, Ivan
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Chromista
Ciliophora
Heterotrichea
Heterotrichida
Clevelandellidae
Clevelandella
Clevelandella sidi

description: Clevelandella sidi sp.nov.
ZooBank registration: urn:lsid:zoobank.org:act: 312386D2- BE75-45F2-AEE7-E24D28C77719.
Diagnosis: Dorsoventrally flattened Clevelandella with average length in protargol preparations 142 µm, mean 52 adoral membranelles, broad, inverted teardrop-shaped macronucleus, and entirely diplostichomonad POM.
Etymology: Named in honour of exceptional terrain entomologist and leader of PNG expedition, Dr Petr ‘Sidi’ Stiblík.
Typelocality: Cambodia, near Mondulkiri NP, N12.383, E107.176.
Type host: Salganea rugulata Saussure, 1895.
Type material: A protargol-impregnated holotype cell (Fig. 15C) marked in a black circle on a slide deposited in the collection of the National Museum of the Czech Republic, Prague, Czech Republic, inventory number P6E 5438.
description: Published as part of Kotyk, Michael, Bourland, William A., Soviš, Matyáš, Méndez-Sánchez, Daniel, Škaloud, Pavel, Varadínová, Zuzana Kotyková & Čepička, Ivan, 2024, Morphology maưers: congruence of morphology and phylogeny in the integrative taxonomy of Clevelandellidae (Ciliophora: Armophorea) with description of six new species, pp. 1-51 in Zoological Journal of the Linnean Society 202 (1) on page 48, DOI: 10.1093/zoolinnean/zlad154, http://zenodo.org/record/14541981
Double Magnon-Roton Excitations in the Triangular-Lattice Spin Supersolid
Creators: name: Gao, Yuan
name: Zhang, Chuandi
name: Xiang, Junsen
name: Yu, Dehong
name: Lu, Xingye
name: Sun, Peijie
name: Jin, Wentao
name: Su, Gang
name: Li, Wei
Publisher: name: arXiv
Year: 2024
Subjects: subject: Strongly Correlated Electrons (cond-mat.str-el)
subject: FOS: Physical sciences

description: Supersolid is an exotic quantum state of matter that spontaneously hosts the features of both solid and superfluid, which breaks the translation and U(1) gauge symmetries. Here we study the spin dynamics in the triangular-lattice compound Na$_2$BaCo(PO$_4$)$_2$, which is revealed in [Xiang et al., Nature 625, 270-275 (2024)] as a quantum magnetic analog of supersolid. We simulate the easy-axis Heisenberg model with tensor network approach and uncover unique dynamic traits. These features are manifested in two branches of excitations that can be associated with the spin solidity and superfluidity, respectively. One branch contains the U(1) Goldstone and roton modes, while the other comprises pseudo-Goldstone and roton modes. The gapless Goldstone modes of the in-plane superfluid order are confirmed by our inelastic neutron scattering measurements. Together with the evident out-of-plane solid order indicated by the magnetic Bragg peaks, our findings provide spectroscopic evidence for spin supersolidity in this easy-axis antiferromagnet. Akin to the role of phonon-roton modes -- Landau elementary excitations -- in shaping the helium superfluid thermodynamics, the intriguing double magnon-roton dispersion here determines the low-temperature thermodynamics of spin supersolid down to sub-Kelvin regime, explaining the recently observed giant magnetocaloric effect in Na$_2$BaCo(PO$_4$)$_2$.
description: 8+9 pages, 4+9 figures
Additional file 1: of Benefits of an expanded use of plasma exchange for anti-neutrophil cytoplasmic antibody-associated vasculitis within a dedicated clinical service
Creators: name: Dhaun, Neeraj
name: Saunders, Andrew
name: Bellamy, Christopher
name: Gallardo, Rocío
name: Manson, Lynn
name: Kluth, David
Publisher: name: Figshare
Year: 2015
Subjects: subject: Medicine
subject: Cell Biology
subject: Molecular Biology
subject: Physiology
subject: FOS: Biological sciences
subject: Biotechnology
subject: Immunology
subject: FOS: Clinical medicine
subject: Cancer
subject: Hematology
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Supplementary Methods. Table S1. Comparison of patients receiving rituximab or cyclophosphamide (CYC) for induction. Data are shown as number of patients (%), mean ± standard deviation or median and IQR. Table S2. Comparison of rituximab dosing, cumulative glucocorticoid dose at 3 months, remission, relapse and survival in patients <70 and ≥70 years of age. Data are shown as number of patients (%), mean ± standard deviation or median and IQR. (DOCX 114 kb)
Phatnotis legata Meyrick 1913
Creators: name: Das, Apurva
name: Pathania, Prakash C.
name: Singh, Navneet
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Lepidoptera
Lecithoceridae
Phatnotis
Phatnotis legata

description: 139. Phatnotis legata Meyrick, 1913: 181
Type locality: India, Karnataka, N. Coorg
Distribution: Indian records: Karnataka.
Host: Larvae take the leaves of Santalum album (Santalaceae) as their food.
description: Published as part of Das, Apurva, Pathania, Prakash C. & Singh, Navneet, 2022, A catalogue of Indian Lecithoceridae (Lepidoptera: Gelechioidea), pp. 56-78 in Zootaxa 5165 (1) on page 72, DOI: 10.11646/zootaxa.5165.1.2, http://zenodo.org/record/6825526
Clevelandella panesthiae
Creators: name: Kotyk, Michael
name: Bourland, William A.
name: Soviš, Matyáš
name: Méndez-Sánchez, Daniel
name: Škaloud, Pavel
name: Varadínová, Zuzana Kotyková
name: Čepička, Ivan
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Chromista
Ciliophora
Heterotrichea
Heterotrichida
Clevelandellidae
Clevelandella
Clevelandella panesthiae

description: Clevelandella panesthiae (Kidder, 1937)
Synonyms: Emmaninius papilloris Yamasaki, 1939; Clevelandella papilloris (Yamasaki, 1939); Clevelandella nova Pecina and Vďačný, 2022.
description: Published as part of Kotyk, Michael, Bourland, William A., Soviš, Matyáš, Méndez-Sánchez, Daniel, Škaloud, Pavel, Varadínová, Zuzana Kotyková & Čepička, Ivan, 2024, Morphology maưers: congruence of morphology and phylogeny in the integrative taxonomy of Clevelandellidae (Ciliophora: Armophorea) with description of six new species, pp. 1-51 in Zoological Journal of the Linnean Society 202 (1) on page 49, DOI: 10.1093/zoolinnean/zlad154, http://zenodo.org/record/14541981
title: "Sacra Famiglia", derivazione da Sanzio Raffaello, inv. 368 (ITA/EN)
title: Scheda catalogo digitale Borghese
titleType: AlternativeTitle
Creators: name: Minozzi, Marina
name: Galleria Borghese
Publisher: name: Galleria Borghese
Year: 2024

description: Pubblicazione delle schede delle opere conservate nel museo ed estratte dal catalogo digitale di Galleria Borghese, nelle due versioni esistenti online, in italiano e inglese.

Collegamento al catalogo digitale:

ITA: https://www.collezionegalleriaborghese.it/

EN: https://www.collezionegalleriaborghese.it/en
Resolving chaperone-assisted protein folding on the ribosome at the peptide level.
Creators: name: Wales, Thomas E
name: Pajak, Aleksandra
name: Roeselová, Alžběta
name: Shivakumaraswamy, Santosh
name: Howell, Steven
name: Kjær, Svend
name: Hartl, F Ulrich
name: Engen, John R
name: Balchin, David
Publisher: name: The Francis Crick Institute
Year: 2024
Subjects: classificationCode: Chemical Biology & High Throughput
subject: Chemical Biology & High Throughput
classificationCode: Computational & Systems Biology
subject: Computational & Systems Biology
classificationCode: Biochemistry & Proteomics
subject: Biochemistry & Proteomics
classificationCode: Structural Biology & Biophysics
subject: Structural Biology & Biophysics
classificationCode: Imaging
subject: Imaging

description: Protein folding in vivo begins during synthesis on the ribosome and is modulated by molecular chaperones that engage the nascent polypeptide. How these features of protein biogenesis influence the maturation pathway of nascent proteins is incompletely understood. Here, we use hydrogen-deuterium exchange mass spectrometry to define, at peptide resolution, the cotranslational chaperone-assisted folding pathway of Escherichia coli dihydrofolate reductase. The nascent polypeptide folds along an unanticipated pathway through structured intermediates not populated during refolding from denaturant. Association with the ribosome allows these intermediates to form, as otherwise destabilizing carboxy-terminal sequences remain confined in the ribosome exit tunnel. Trigger factor binds partially folded states without disrupting their structure, and the nascent chain is poised to complete folding immediately upon emergence of the C terminus from the exit tunnel. By mapping interactions between the nascent chain and ribosomal proteins, we trace the path of the emerging polypeptide during synthesis. Our work reveals new mechanisms by which cellular factors shape the conformational search for the native state.
Axial Gravitational Perturbations of Slowly-Rotating Compact Objects in General Relativity and Beyond
Creators: name: Feng, Xing-Hui
name: Peng, Jun
Publisher: name: arXiv
Year: 2024
Subjects: subject: General Relativity and Quantum Cosmology (gr-qc)
subject: High Energy Physics - Theory (hep-th)
subject: FOS: Physical sciences

description: We study the axial gravitational perturbations of slowly-rotating compact objects which are assumed to be supported by anisotropic fluids. We find that the gravitational perturbations decouple from the matter perturbations for axial sectors. We obtain a master wave equation whose potential is fully determined by the metric functions. This equation makes the calculations of gravitational QNMs for rotating compact objects extremely easy in specific background configurations.
description: latex, 14 pages, 1 figure; published version
Philharmonia Gozmany 1978
Creators: name: Das, Apurva
name: Pathania, Prakash C.
name: Singh, Navneet
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Lepidoptera
Lecithoceridae
Philharmonia

description: Genus Philharmonia Gozmány, 1978: 248
Type species: Philharmonia paratona Gozmány, 1978, by original designation
description: Published as part of Das, Apurva, Pathania, Prakash C. & Singh, Navneet, 2022, A catalogue of Indian Lecithoceridae (Lepidoptera: Gelechioidea), pp. 56-78 in Zootaxa 5165 (1) on page 72, DOI: 10.11646/zootaxa.5165.1.2, http://zenodo.org/record/6825526
MOESM2 of Global priorities for research and the relative importance of different research outcomes: an international Delphi survey of malaria research experts
Creators: name: Jo-Ann Mulligan
name: Lesong Conteh
Publisher: name: Figshare
Year: 2016
Subjects: subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Science Policy
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Additional file 2. Surprising developments in malaria research: free text responses.
Boletim Fármacos: Farmacovigilância 2024; 1(2)
Creators: name: Salud y Fármacos
name: Homedes, Nuria
Publisher: name: Salud y Fármacos
Year: 2024
Subjects: Medicamentos
Farmacovigilância
Alertas, segurança e recalls
Interações medicamentosas
Interações medicamentosas com a medicina tradicional
Interações entre medicamentos e alimentos
Reações adversas
Solicitações e retiradas do mercado
Solicitações e mudanças na rotulagem
Precauções

description: O Boletín Fármacos é um boletim eletrônico da organização Salud y Fármacos (https://www.saludyfarmacos.org/) publicado quatro vezes por ano: fevereiro, maio, agosto e novembro.

O Boletín Fármacos: Farmacovigilancia inclui artigos e notas informativas sobre a segurança dos medicamentos. Abrange tópicos de pesquisa; alertas para solicitações e/ou recalls de medicamentos; alterações nos rótulos dos medicamentos; informações sobre possíveis interações entre medicamentos, medicamentos e medicamentos, medicina tradicional, medicamentos e alimentos, reações adversas; e alguns outros relatórios de farmacovigilância. Essa seção geralmente inclui traduções dos boletins mais conhecidos: Worst Pills, Best Pills (Public Citizen), Therapeutics Initiative e Prescrire.

Este documento corresponde a 2024, Vol. 1, No. 2.
MOESM1 of Characterization of blood dendritic and regulatory T cells in asymptomatic adults with sub-microscopic Plasmodium falciparum or Plasmodium vivax infection
Creators: name: Kho, Steven
name: Marfurt, Jutta
name: Handayuni, Irene
name: Zuleima Pava
name: Rintis Noviyanti
name: Kusuma, Andreas
name: Piera, Kim
name: Burdam, Faustina
name: Kenangalem, Enny
name: Lampah, Daniel
name: Engwerda, Christian
name: Poespoprodjo, Jeanne
name: Ric Price
name: Anstey, Nicholas
name: Minigo, Gabriela
name: Woodberry, Tonia
Publisher: name: Figshare
Year: 2016
Subjects: subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Physiology
subject: Immunology
subject: FOS: Clinical medicine
subject: Cancer
subject: Plasma Physics
subject: Hematology
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: 60506 Virology
subject: Computational Biology

description: Additional file 1. List of antibody panels. The table provided represents the antibody panels used to characterize DC and Treg cells by flow cytometry.
Qubit encoding for a mixture of localized functions
Creators: name: Kosugi, Taichi
name: Daimon, Shunsuke
name: Nishi, Hirofumi
name: Tsuneyuki, Shinji
name: Matsushita, Yu-ichiro
Publisher: name: arXiv
Year: 2024
Subjects: subject: Quantum Physics (quant-ph)
subject: Strongly Correlated Electrons (cond-mat.str-el)
subject: FOS: Physical sciences

description: One of the crucial generic techniques for quantum computation is amplitude encoding. Although several approaches have been proposed, each of them often requires exponential classical-computational cost or an oracle whose explicit construction is not provided. Given the growing demands for practical quantum computation, we develop moderately specialized encoding techniques that generate an arbitrary linear combination of localized complex functions. We demonstrate that $n_{\mathrm{loc}}$ discrete Lorentzian functions as an expansion basis set lead to eficient probabilistic encoding, whose computational time is $\mathcal{O}( \max ( n_{\mathrm{loc}}^2 \log n_{\mathrm{loc}},n_{\mathrm{loc}}^2 \log n_q, n_q ))$ for $n_q$ data qubits equipped with $\log_2 n_{\mathrm{loc}}$ ancillae. Furthermore, amplitude amplification in combination with amplitude reduction renders it deterministic analytically with controllable errors and the computational time is reduced to $\mathcal{O}( \max ( n_{\mathrm{loc}}^{3/2} \log n_{\mathrm{loc}}, n_{\mathrm{loc}}^{3/2} \log n_q, n_q )).$ We estimate required resources for applying our scheme to quantum chemistry in real space. We also show the results on real superconducting quantum computers to confirm the validity of our techniques.
description: 12 figures
Outcome after veno‐arterial extracorporeal membrane oxygenation in elderly patients: A 14‐year single‐center experience
Creators: name: Provaznik, Zdenek
name: Philipp, Alois
name: Müller, Thomas
name: Kostiantyn, Kozakov
name: Lunz, Dirk
name: Schmid, Christof
name: Floerchinger, Bernhard
Publisher: name: Universität Regensburg
Year: 2022
Subjects: subject: extracorporeal membrane oxygenation, long-term outcome, low cardiac output, resuscitation, survival
subject: 610 Medizin

description: Background
Use of veno-arterial extracorporeal membrane oxygenation (VA-ECMO) in elderly patients is controversial because of presumed poor outcome. Our primary aim was to determine the influence of advanced age on short- and long-term outcome; the secondary aim was to analyze risk factors for impaired outcome.
Methods
Between January 2006 and June 2020, 645 patients underwent VA-ECMO implantation in our department. The patients were categorized into four groups:<50, 50–59.9, 60–69.9 and ≥70 years old. Data were retrospectively analyzed for short- and long-term outcome. Risk factors for in-hospital mortality and mortality during follow-up were assessed using multivariate regression analysis.
Results
VA-ECMO support duration was comparable in all age groups (median 3 days). Weaning rates were 60.8%/n = 104 (<50 years), 51.4%/n = 90 (50–59.9 years), 58.8%/n = 107 (60–69.9), and 67.5%/n = 79 (≥70, p = 0.048). Hospital mortality was highest in the patients aged 50–59.9 years (68%/n = 119), but not in the elderly patients (60–69.9, ≥70:62.1%/n = 113, 58,1%/n = 68). At discharge, the cerebral performance category scores were superior in the patients <50 years. Multivariate logistic regression analysis revealed chronic kidney failure requiring hemodialysis, duration of cardiopulmonary resuscitation, and elevated blood lactate levels before VA-ECMO, but not age as predictors of in-hospital mortality. Cox's regression disclosed age as relevant risk factor for death during follow-up. The patients' physical ability was comparable in all age groups.
Conclusion
VA-ECMO support should not be declined in patients only because of advanced age. Mortality and neurological status at hospital discharge and during follow-up were comparable in all age groups.
Additional file 1: Figure S1. of The structure and diversity of human, animal and environmental resistomes
Creators: name: Pal, Chandan
name: Bengtsson-Palme, Johan
name: Kristiansson, Erik
name: D. Larsson
Publisher: name: Figshare
Year: 2016
Subjects: subject: Space Science
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Molecular Biology
subject: Evolutionary Biology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Heat map showing the most abundant antibiotic resistance genes across environments. Figure S2. Relative abundance and richness of antibiotic resistance genes (ARGs) (per 16S rRNA) in air samples. Figure S3. Distribution of antibiotic resistance genes and bacterial genera among three ecologically distinct compartmentsâ  humans, animals and external environments. Figure S4. Correlations between richness of resistance genes. Figure S5. Heat map showing abundance of different classes of mobile genetic elements (integron-associated integrases and ISCR transposases) across environments. Figure S6. Relative proportion of classified and unclassified bacteria across environments. Figure S7. Heat map showing the most abundant bacterial genera across environments. (PDF 217Â kb)
Philharmonia filiale Gozmany 2002
Creators: name: Das, Apurva
name: Pathania, Prakash C.
name: Singh, Navneet
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Lepidoptera
Lecithoceridae
Philharmonia
Philharmonia filiale

description: 140. Philharmonia filiale Gozmány, 2002: 36
Type locality: India, Rajasthan, Bharatpur, Keoladeo National Park
Distribution: Indian records: Rajasthan.
description: Published as part of Das, Apurva, Pathania, Prakash C. & Singh, Navneet, 2022, A catalogue of Indian Lecithoceridae (Lepidoptera: Gelechioidea), pp. 56-78 in Zootaxa 5165 (1) on pages 72-73, DOI: 10.11646/zootaxa.5165.1.2, http://zenodo.org/record/6825526
Additional file 6: Figure S2. of Similar striatal gene expression profiles in the striatum of the YAC128 and HdhQ150 mouse models of Huntingtonâ  s disease are not reflected in mutant Huntingtin inclusion prevalence
Creators: name: Zubeyde Bayram-Weston
name: Stone, Timothy
name: Giles, Peter
name: Elliston, Linda
name: Nari Janghra
name: Higgs, Gemma
name: Holmans, Peter
name: Dunnett, Stephen
name: Brooks, Simon
name: Jones, Lesley
Publisher: name: Figshare
Year: 2015
Subjects: subject: Biochemistry
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Neuroscience
subject: Physiology
subject: Evolutionary Biology
subject: 69999 Biological Sciences not elsewhere classified
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: Developmental Biology
subject: Cancer
subject: 111714 Mental Health
subject: FOS: Health sciences

description: Analysis of the overlap of changes in gene expression in the YAC128 striatum at A. 6Â months of age B. 12 months of age and C. 18 months of age compared with other HD model mouse striata and human brain. Frequency represents the fraction of the top 200 HdhQ150 expression changes that map to a particular bin of ranked data (1000 genes per bin) in the other dataset, which is then split to identify concordant or discordant direction of expression change. A higher frequency of concordant (green) rather than discordant (red) in the first bins indicates a similarity between the YAC128 caudate and other model or human HD gene expression signature. (PDF 59 kb)
Clevelandella parapanesthiae
Creators: name: Kotyk, Michael
name: Bourland, William A.
name: Soviš, Matyáš
name: Méndez-Sánchez, Daniel
name: Škaloud, Pavel
name: Varadínová, Zuzana Kotyková
name: Čepička, Ivan
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Chromista
Ciliophora
Heterotrichea
Heterotrichida
Clevelandellidae
Clevelandella
Clevelandella parapanesthiae

description: Clevelandella parapanesthiae (Kidder, 1937)
Synonyms: Clevelandella bipanesthiae Pecina and Vďačný, 2022; C.biparapanesthiae Pecina and Vďačný, 2022; C. triparapanesthiae Pecina and Vďačný, 2022.
description: Published as part of Kotyk, Michael, Bourland, William A., Soviš, Matyáš, Méndez-Sánchez, Daniel, Škaloud, Pavel, Varadínová, Zuzana Kotyková & Čepička, Ivan, 2024, Morphology maưers: congruence of morphology and phylogeny in the integrative taxonomy of Clevelandellidae (Ciliophora: Armophorea) with description of six new species, pp. 1-51 in Zoological Journal of the Linnean Society 202 (1) on page 49, DOI: 10.1093/zoolinnean/zlad154, http://zenodo.org/record/14541981
Pompographa Gozmany 1971
Creators: name: Das, Apurva
name: Pathania, Prakash C.
name: Singh, Navneet
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Lepidoptera
Lecithoceridae
Pompographa

description: Genus Pompographa Gozmány, 1971: 252
Type species: Brachmia philosopha Meyrick, 1911a, by original designation
description: Published as part of Das, Apurva, Pathania, Prakash C. & Singh, Navneet, 2022, A catalogue of Indian Lecithoceridae (Lepidoptera: Gelechioidea), pp. 56-78 in Zootaxa 5165 (1) on page 73, DOI: 10.11646/zootaxa.5165.1.2, http://zenodo.org/record/6825526
Pompographa philosopha Brachmia
Creators: name: Das, Apurva
name: Pathania, Prakash C.
name: Singh, Navneet
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Lepidoptera
Lecithoceridae
Pompographa
Pompographa philosopha

description: 141. Pompographa philosopha (Meyrick, 1911a: 708) (Brachmia)
Type locality: India, Assam, Khasi Hills [Now in Meghalaya]
Distribution: Indian records: Meghalaya.
description: Published as part of Das, Apurva, Pathania, Prakash C. & Singh, Navneet, 2022, A catalogue of Indian Lecithoceridae (Lepidoptera: Gelechioidea), pp. 56-78 in Zootaxa 5165 (1) on page 73, DOI: 10.11646/zootaxa.5165.1.2, http://zenodo.org/record/6825526
Additional file 3: Table S3. of Opening the “black box” of nodD3, nodD4 and nodD5 genes of Rhizobium tropici strain CIAT 899
Creators: name: Cerro, Pablo Del
name: Rolla-Santos, Amanda
name: Gomes, Douglas
name: Marks, Bettina
name: Espuny, María Del Rosario
name: Rodríguez-Carvajal, Miguel
name: Soria-Díaz, María
name: Nakatani, André
name: Hungria, Mariangela
name: Ollero, Francisco
name: Megías, Manuel
Publisher: name: Figshare
Year: 2015
Subjects: subject: Biochemistry
subject: Space Science
subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Science Policy
subject: Plant Biology

description: Nod Factor structure biosynthesized in the presence of 300 mM NaCl by wild type CIAT 899 and derivatives. (DOC 81 kb)
Clevelandella kidderi Mandal and Nair 1974
Creators: name: Kotyk, Michael
name: Bourland, William A.
name: Soviš, Matyáš
name: Méndez-Sánchez, Daniel
name: Škaloud, Pavel
name: Varadínová, Zuzana Kotyková
name: Čepička, Ivan
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Chromista
Ciliophora
Heterotrichea
Heterotrichida
Clevelandellidae
Clevelandella
Clevelandella kidderi

description: Clevelandella kidderi Mandal and Nair, 1974
Synonym: Clevelandella lynni Pecina and Vďačný, 2020
description: Published as part of Kotyk, Michael, Bourland, William A., Soviš, Matyáš, Méndez-Sánchez, Daniel, Škaloud, Pavel, Varadínová, Zuzana Kotyková & Čepička, Ivan, 2024, Morphology maưers: congruence of morphology and phylogeny in the integrative taxonomy of Clevelandellidae (Ciliophora: Armophorea) with description of six new species, pp. 1-51 in Zoological Journal of the Linnean Society 202 (1) on page 49, DOI: 10.1093/zoolinnean/zlad154, http://zenodo.org/record/14541981
Granulocyte concentrate splitting does not affect phenotype and function
Creators: name: Koo, Sebastian
name: Offner, Robert
name: Haile, Sophie‐Marie
name: Brosig, Andreas
name: Hähnel, Viola
name: Gruber, Michael
name: Burkhardt, Ralph
name: Ahrens, Norbert
Publisher: name: Universität Regensburg
Year: 2022
Subjects: subject: distribution, granulocyte concentrates, granulocytes, modified fluid gelatin, neutrophils, separation, storage, transfusion
subject: 610 Medizin

description: Background:
More granulocyte concentrates (GCs) could be produced for
more patients from the same donor if apheresis bags were split and stored for
longer periods of time. Hence, we tested the hypothesis that splitting and
extension of storage of GCs do not impair granulocyte function or viability.
Study Design and Methods: 
Granulocyte apheresis concentrates were produced
using modified fluid gelatin as a separation enhancer, split into two portions, and
stored for 24 and 48 h. Granulocyte function, represented by cell migration, reactive
oxygen species (ROS) production, and neutrophil extracellular trap formation
(NETosis), was measured by live-cell imaging. ROS production, adhesive surface
protein expression, and viability were measured by flow cytometry.
Results: 
Splitting had no effect on any of the tested parameters. After 24 h of
storage, live-cell imaging showed no significant difference in migration, time
to maximum ROS production, time to half-maximum NETosis, viability, or
CD11b expression, but ROS production induced by phorbol 12-myristate
13-acetate (PMA) decreased from an initial median fluorescence intensity of
1775–590 artificial units. After 48 h, PMA-induced ROS production, viability,
and migration declined, as reflected by decreases in median total distance
(119 vs. 63.5 μm) and median Euclidean distance (30.75 vs. 14.3 μm).
Conclusion: 
Splitting GC products has no effect on granulocyte viability or
function, but extended storage >24 h does compromise granulocyte function.
The findings confirm that GCs should be transfused within 24 h of collection.
Longer storage cannot be recommended.