Abrochtha kingi Jr &amp; Ricci &amp; Melone &amp; Fontaneto 2011, SP. NOV.
Creators: name: Jr, C. William Birky
name: Ricci, Claudia
name: Melone, Giulio
name: Fontaneto, Diego
Publisher: name: Zenodo
Year: 2011
Subjects: Biodiversity
Taxonomy
Animalia
Rotifera
Eurotatoria
Bdelloidea
Philodinavidae
Abrochtha
Abrochtha kingi

description: <i>ABROCHTHA KINGI</i> SP. NOV. <i>Type material:</i> Slide mounted holotype and ten paratypes, along with digital still and video photographs, deposited in the Academy of Natural Sciences, Philadelphia, accession numbers 2061 (holotype) and 2062 to 2071 (paratypes); DNA sequence of <i>COI</i> deposited in GenBank (accession number GU989444). All specimens were isolated from a clonal lab culture obtained from one individual named Shelf 1.3, collected from the type locality on 29 July 2002 by C. W. B. <i>Type locality:</i> Shelf Lake Number 3, Snowy Range, Wyoming, USA; altitude 3316 m, 41°22 <i>′</i> 43.2 <i>′′</i> N, 106°17 <i>′</i> 20.9 <i>′′</i> W. <i>Etymology:</i> The new species is dedicated to the lifelong studies of Charles Everett King, from Oregon State University, on ecology and genetics of rotifers. <i>Differential diagnosis:</i> No unambiguous discrimination is possible between this species and <i>A. meselsoni</i> so far, except from its <i>COI</i> sequence. Ramus length was significantly smaller than in <i>A. meselsoni</i>, but with a wide overlap; therefore, such difference in size is useless for taxonomic purposes. For <i>A. kingi</i>, we determined the mean ramus length from SEMs of the left and right ramus of 13 individuals. These data were compared to measurements of 40 trophi of <i>A. meselsoni</i>: 22 from the type clone (Angl2.7b), eight animals of clone Angl2.8, and ten from clone Angl1.1. Ramus length was significantly different amongst clones [analysis of variance (ANOVA) test: <i>F</i> 3,49 = 3.80, <i>P</i> = 0.015]; a post-hoc Tukey’s honestly significant difference (HSD) test gave a significant difference between the type clone of <i>A. meselsoni</i> and <i>A. kingi</i> (<i>P</i> = 0.002), but no significant differences amongst the different clones of <i>A. meselsoni</i> (<i>P</i> = 0.34 and 0.61). <i>General morphology:</i> The external morphology (Figs 5A, 6) completely overlaps with that of <i>A. meselsoni</i>. Refer to the previous description for its morphological features. Additionally, trophi shape and number of teeth are not distinguishable from <i>A. meselsoni</i> (Fig. 4C, D). 730 C. W. BIRKY <i>ET AL</i>. <i>Measurements:</i> Body measures overlap those of <i>A. meselsoni</i> and of <i>A. kingi</i>, but some animals reached larger size: total length, 360 Mm; trunk length, 210 Mm; trunk width, 110 Mm; head width, 60 Mm. The ramus length measured from SEM is 16.17 ± 0.63 (SD) Mm (<i>N</i> = 26). <i>Distribution and ecology:</i> This new species is known only from the type locality, Shelf Lake Number 3 in the Snowy Range of Wyoming. This is a small shallow lake in the Alpine Zone at an altitude of 3316 m that is filled with water from snowmelt and rain in summer, but covered by deep snow and at least partly frozen in winter. <i>DNA barcoding:</i> The sequence of partial COI mtDNA of animals collected from the same clonal laboratory culture Shelf1.3 has been deposited in GenBank with accession number GU989444.
description: Published as part of <i>Jr, C. William Birky, Ricci, Claudia, Melone, Giulio &amp; Fontaneto, Diego, 2011, Integrating DNA and morphological taxonomy to describe diversity in poorly studied microscopic animals: new species of the genus Abrochtha Bryce, 1910 (Rotifera: Bdelloidea: Philodinavidae), pp. 723-734 in Zoological Journal of the Linnean Society 161 (4)</i> on pages 729-730, DOI: 10.1111/j.1096-3642.2010.00674.x, http://zenodo.org/record/5440189