title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ Ð½ÐµÐ¾Ð½Ð°ÑÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð²Ð¾ÑÐ¿Ð°Ð»Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ ÑÑÑÐµÑÑÐ°, Ð·Ð°Ð´Ð°Ð²Ð°ÐµÐ¼Ð¾Ð³Ð¾ Ð²Ð²ÐµÐ´ÐµÐ½Ð¸ÑÐ¼Ð¸ ÐÐ-1Î² Ð¸Ð»Ð¸ ÐÐÐ¡, Ð½Ð° ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð³ÐµÐ½Ð¾Ð² Ð½ÐµÐ¹ÑÐ¾Ð¿Ð»Ð°ÑÑÐ¸ÑÐ½Ð¾ÑÑÐ¸ Ð² Ð¾Ð±Ð»Ð°ÑÑÑÑ Ð¼Ð¾Ð·Ð³Ð° ÐºÑÑÑ Ð¿Ð¾ÑÐ¾Ð´Ñ ÐÐ¸ÑÑÐ°Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Effects of neonatal inflammatory stress induced by injections IL-1Î² or LPS on neuroplasticity gene expression in brain structures of Wistar rats
Creators: name: Ð¨Ð¸ÑÐ¾ÐºÐ¾Ð², ÐÐ³Ð¾Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Ð²Ð¾ÑÐ¿Ð°Ð»Ð¸ÑÐµÐ»ÑÐ½ÑÐ¹ ÑÑÑÐµÑÑ
Ð¿ÑÐ¾Ð²Ð¾ÑÐ¿Ð°Ð»Ð¸ÑÐµÐ»ÑÐ½ÑÐµ ÑÐ°ÐºÑÐ¾ÑÑ
ÐÐ-1Î²
ÐÐÐ¡
Ð½ÐµÐ¹ÑÐ¾Ð¿Ð»Ð°ÑÑÐ¸ÑÐ½Ð¾ÑÑÑ
inflammatory stress
proinflammatory factors
IL-1Î²
LPS
neuroplasticity

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description: Inflammatory stress in the early postnatal ontogeny can cause disturbances in CNS development and facilitate predisposition towards various cognitive impairments. Such stress can be modeled in rats by IL-1Î² or LPS administration. Possible mechanism mediating such disturbances is alteration in neuroplasticity genes expression.The aim of this study was to compare effects of neonatal inflammatory stress models, set by intraperitoneal injections of moderately pyrogenic doses of proinflammatory factors IL-1Î² or LPS to Wistar rats during 3rd postnatal week, on relative expression levels of neuroplasticity genes Bdnf, Fgf2 and on the ratio of expression levels of Mmp9 and Timp1 in the tissues of dorsal hippocampus (DH and medial prefrontal cortex (mPFC), both in pupsâ and adult animalsâ brain.Gene expression levels were studied using RT-qPCR in the DH and mPFC samples obtained 2 hours and 2,5 months after the final injection.In order to achieve greater reliability of qPCR results, multiplex reference gene stability testing systems were developed and used in this study. Such most stably expressed housekeeping genes were determined for the experimental setting in question and used as reference genes.It was established, that IL-1Î² administrations cause Fgf2 mRNA upregulation in pupsâ mPFC. LPS administration caused the decrease in Mmp9/Timp1 mRNA ratio in the DH of adult animals, and decreased relative expression of Fgf2 both in DH and mPFC of adult animals.