title: ÐÑÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð¸Ð· Ð¿ÑÐ¸ÑÐ¾Ð´Ð½ÑÑ Ð±Ð¸Ð¾ÑÐµÐ½Ð¾Ð·Ð¾Ð² Ð¸ Ñ Ð°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ° Ð±Ð°ÐºÑÐµÑÐ¸Ð¹, Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¸ÑÑÑÑÐ¸Ñ Ð¿ÐµÐ½ÑÐ°Ð´ÐµÐºÐ°Ð½
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Isolation from natural biocenoses and characterization of bacteria metabolizing pentadecane
Creators: name: Ð¯ÐºÑÑÐ¸Ð½Ð°, ÐÐ¸ÐºÑÐ¾ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Ð¿ÐµÐ½ÑÐ°Ð´ÐµÐºÐ°Ð½
Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¼
Ð¾ÐºÐ¸ÑÐ»ÐµÐ½Ð¸Ðµ
Ð±Ð¸Ð¾Ð¿Ð»ÐµÐ½ÐºÐ¸
Ð±Ð¸Ð¾ÑÐµÐ¼ÐµÐ´Ð¸Ð°ÑÐ¸Ñ
pentadecane
metabolism
oxidation
biofilms
bioremediation

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¸Ð· Ð¿Ð¾ÑÐ²ÐµÐ½Ð½ÑÑ Ð¿ÑÐ¾Ð± Ð¸ Ð¿ÑÐ¾Ð±Ñ Ð¼Ð¾ÑÑÐºÐ¾Ð¹ Ð²Ð¾Ð´Ñ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹, Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¸ÑÑÑÑÐ¸Ñ Ð¾Ð´Ð¸Ð½ Ð¸Ð· ÐºÐ¾Ð¼Ð¿Ð¾Ð½ÐµÐ½ÑÐ¾Ð² Ð½ÐµÑÑÑÐ½ÑÑ Ð·Ð°Ð³ÑÑÐ·Ð½ÐµÐ½Ð¸Ð¹ â Ð¿ÐµÐ½ÑÐ°Ð´ÐµÐºÐ°Ð½, Ð° ÑÐ°ÐºÐ¶ÐµÂ  ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐµ Ð¸Ñ ÑÐ²Ð¾Ð¹ÑÑÐ². ÐÐ°Ð´Ð°ÑÐ¸, ÑÐµÑÐ°ÐµÐ¼ÑÐµ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: - Ð¿ÑÐ¾Ð°Ð½Ð°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°ÑÑ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÑ Ð¾ Ð±Ð°ÐºÑÐµÑÐ¸ÑÑ, Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÑÑÐ¸Ñ Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¼ Ð´Ð»Ð¸Ð½Ð½Ð¾ÑÐµÐ¿Ð¾ÑÐµÑÐ½ÑÑ ÑÐ³Ð»ÐµÐ²Ð¾Ð´Ð¾ÑÐ¾Ð´Ð¾Ð² Ð¸ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼ ÑÑÐ¸Ñ ÑÐµÐ°ÐºÑÐ¸Ð¹; - Ð¸Ð·ÑÑÐ¸ÑÑ Ð¼ÐµÑÐ¾Ð´Ñ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ Ð½Ð°ÐºÐ¾Ð¿Ð¸ÑÐµÐ»ÑÐ½ÑÑ Ð¸ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ ÑÐ¸ÑÑÑÑ ÐºÑÐ»ÑÑÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹; - Ð¿Ð¾Ð»ÑÑÐ¸ÑÑ Ð½Ð°ÐºÐ¾Ð¿Ð¸ÑÐµÐ»ÑÐ½ÑÐµ ÐºÑÐ»ÑÑÑÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ Ð¸Ð· Ð¿Ð¾ÑÐ²ÐµÐ½Ð½ÑÑ Ð¿ÑÐ¾Ð± Ð¸ Ð¿ÑÐ¾Ð±Ñ Ð¼Ð¾ÑÑÐºÐ¾Ð¹ Ð²Ð¾Ð´Ñ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ Ð³ÐµÐ¾Ð³ÑÐ°ÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð·Ð¾Ð½; - Ð²ÑÑÐ²Ð¸ÑÑ Ñ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°ÑÑ Ð¿ÐµÐ½ÑÐ°Ð´ÐµÐºÐ°Ð½ Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ ÐµÐ´Ð¸Ð½ÑÑÐ²ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¸ÑÑÐ¾ÑÐ½Ð¸ÐºÐ° ÑÐ³Ð»ÐµÑÐ¾Ð´Ð° Ð¸ ÑÐ½ÐµÑÐ³Ð¸Ð¸; - Ð¾Ð¿Ð¸ÑÐ°ÑÑ ÐºÑÐ»ÑÑÑÑÐ°Ð»ÑÐ½ÑÐµ Ð¸ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹. ÐÐ±ÑÐµÐºÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â Ð¾Ð±ÑÐ°Ð·ÑÑ Ð¿Ð¾ÑÐ²Ñ, Ð¾ÑÐ¾Ð±ÑÐ°Ð½Ð½ÑÐµ Ð½Ð° Ð¾ÑÑÑÐ¾Ð²Ð°Ñ ÐÐ¸Ð¿Ñ, ÐÑÐºÑÑ (ÐÑÐ°Ð»Ð¸Ñ) Ð¸ Ñ ÑÐµÑÑÐ¸ÑÐ¾ÑÐ¸Ð¸ ÐÐµÐ½ÑÐºÐ¾Ð²ÑÐºÐ¾Ð³Ð¾ ÑÐ¸Ð»Ð¸Ð°Ð»Ð° ÐÐ³ÑÐ¾ÑÐ¸Ð·Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð½Ð°ÑÑÐ½Ð¾-Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÑÐºÐ¾Ð³Ð¾ Ð¸Ð½ÑÑÐ¸ÑÑÑÐ° (Ð Ð¾ÑÑÐ¸Ñ) Ð¸ Ð¼Ð¾ÑÑÐºÐ¾Ð¹ Ð²Ð¾Ð´Ñ Ð¸Ð· Ð¿ÑÐ¸Ð±ÑÐµÐ¶Ð½Ð¾Ð¹ Ð¿Ð¾Ð»Ð¾ÑÑ Ð¾ÑÑÑÐ¾Ð²Ð° ÐÐ¸Ð¿Ñ. Ð Ð°Ð±Ð¾ÑÐ° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ ÑÐµÐºÑÐ¾ÑÐ° ÑÐºÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð¼Ð¸ÐºÑÐ¾Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ð¸ ÐÐ³ÑÐ¾ÑÐ¸Ð·Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð½Ð°ÑÑÐ½Ð¾-Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÑÐºÐ¾Ð³Ð¾ Ð¸Ð½ÑÑÐ¸ÑÑÑÐ°. ÐÐ½Ð°Ð»Ð¸Ð·Ð¸ÑÑÐµÐ¼ÑÐ¼Ð¸ Ð¿Ð¾ÐºÐ°Ð·Ð°ÑÐµÐ»ÑÐ¼Ð¸ ÑÐ¾ÑÑÐ° Ð½Ð° Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ´Ðµ Ñ Ð¿ÐµÐ½ÑÐ°Ð´ÐµÐºÐ°Ð½Ð¾Ð¼ Ð±ÑÐ»Ð¸: Ð¾Ð¿ÑÐ¸ÑÐµÑÐºÐ°Ñ Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÑ Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ´Ñ, Ð° ÑÐ°ÐºÐ¶Ðµ ÐºÑÐ»ÑÑÑÑÐ°Ð»ÑÐ½ÑÐµ Ð¸ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° Ð±Ð°ÐºÑÐµÑÐ¸Ð¹. Ð ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð² Ð¿Ð¾ÑÐ²Ð°Ñ Ð¾. ÐÐ¸Ð¿Ñ, Ð¾. ÐÑÐºÑÑ Ð¸ ÐÐµÐ½ÑÐºÐ¾Ð²ÑÐºÐ¾Ð³Ð¾ ÑÐ¸Ð»Ð¸Ð°Ð»Ð° ÐÐ³ÑÐ¾ÑÐ¸Ð·Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð½Ð°ÑÑÐ½Ð¾-Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÑÐºÐ¾Ð³Ð¾ Ð¸Ð½ÑÑÐ¸ÑÑÑÐ° Ð¿ÑÐ¸ÑÑÑÑÑÐ²ÑÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¸-Ð´ÐµÑÑÑÑÐºÑÐ¾ÑÑ Ð¿ÐµÐ½ÑÐ°Ð´ÐµÐºÐ°Ð½Ð° Ð¸ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½ÑÐµ Ð±Ð°ÐºÑÐµÑÐ¸Ð¸ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ñ ÑÐ°ÑÑÐ¸ Ð½Ð° Ð¼Ð¸Ð½ÐµÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ´Ðµ ÐÐ¾ÑÐ¾ÑÐ¸Ð»Ð¾Ð²Ð¾Ð¹-ÐÐ¸Ð°Ð½Ð¾Ð²Ð¾Ð¹ Ñ Ð¿ÐµÐ½ÑÐ°Ð´ÐµÐºÐ°Ð½Ð¾Ð¼ Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ ÐµÐ´Ð¸Ð½ÑÑÐ²ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¸ÑÑÐ¾ÑÐ½Ð¸ÐºÐ° ÑÐ³Ð»ÐµÑÐ¾Ð´Ð° Ð¸ ÑÐ½ÐµÑÐ³Ð¸Ð¸, Ð¿ÑÐ¸ ÑÑÐ¾Ð¼ Ð¿Ð¾ÑÐ²ÐµÐ½Ð½ÑÐµ ÐºÑÐ»ÑÑÑÑÑ Ð¾Ð±ÑÐ°Ð·ÑÑÑ Ð±Ð¸Ð¾Ð¿Ð»ÐµÐ½ÐºÐ¸. ÐÐ°ÐºÑÐµÑÐ¸Ð¸, Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½ÑÐµ Ð¸Ð· Ð¿Ð¾ÑÐ²ÐµÐ½Ð½ÑÑ Ð¿ÑÐ¾Ð±, Ð¸Ð¼ÐµÑÑ Ð² Ð¾ÑÐ½Ð¾Ð²Ð½Ð¾Ð¼ ÑÐ°ÑÐ¾Ð²Ð¸Ð´Ð½ÑÑ Ð¸ Ð¿Ð°Ð»Ð¾ÑÐºÐ¾Ð²Ð¸Ð´Ð½ÑÑ ÑÐ¾ÑÐ¼Ñ, Ð° Ð±Ð°ÐºÑÐµÑÐ¸Ð¸, Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½ÑÐµ Ð¸Ð· Ð¾Ð±ÑÐ°Ð·ÑÐ° Ð¼Ð¾ÑÑÐºÐ¾Ð¹ Ð²Ð¾Ð´Ñ Ð¿ÑÐ¸Ð±ÑÐµÐ¶Ð½Ð¾Ð¹ Ð·Ð¾Ð½Ñ Ð¾. ÐÐ¸Ð¿Ñ, Ð¸Ð¼ÐµÑÑ ÑÐ¾ÑÐ¼Ñ ÑÐ¾Ð½ÐºÐ¸Ñ, Ð´Ð»Ð¸Ð½Ð½ÑÑ ÑÐ¿Ð¾ÑÐ¾Ð¾Ð±ÑÐ°Ð·ÑÑÑÐ¸Ñ Ð¿Ð°Ð»Ð¾ÑÐµÐº.
description: This work is devoted to the isolation of bacteria from soil samples and seawater samples that metabolize one of the components of oil pollution â pentadecane, as well as the characteristics of their properties. Tasks solved in the course of the study: - to analyze the literature on bacteria that carry out the metabolism of long-chain hydrocarbons and the mechanism of these reactions; - to study methods of obtaining accumulative and isolation of pure bacterial cultures; - to obtain accumulative cultures of bacteria from soil samples and seawater samples of various geographical zones; - to identify the ability of isolated bacteria to use pentadecane as the only source of carbon and energy; - to describe the cultural and morphological properties of the isolated bacteria. The objects of the study are soil samples taken on the islands of Cyprus, Ischia (Italy) and from the territory of the Menkovsky branch of the Agrophysical Scientific Research Institute (Russia) and sea water from the coastal strip of the island of Cyprus. The work was carried out on the basis of the sector of ecological microbiology of the Agrophysical Research Institute. The analyzed growth indicators on a nutrient medium with pentadecane were: the optical density of the growth medium, as well as cultural and morphological properties of bacteria. The research has shown that in the soils of Cyprus, Ischia and the Menkov branch of the Agrophysical Research Institute, pentadecane destructor bacteria are present and the isolated bacteria are able to grow on the mineral nutrient medium of Voroshilova-Dianova with pentadecane as the only source of carbon and energy, while soil crops they form biofilms. The bacteria isolated from soil samples are mainly spherical and rod-shaped, and the bacteria isolated from a sample of seawater of the coastal zone of o. Cyprus, have the shape of thin, long spore-forming stick.