title: ÐÐ´ÐµÐ½ÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ñ ÑÐ°ÑÑÐµÐ½Ð¸Ð¹ ÑÐµÐ¼ÐµÐ¹ÑÑÐ²Ð° Labiatae â Ð¼Ð¾Ð´ÐµÐ»ÑÐ½Ð¾Ð³Ð¾ ÑÐ°ÑÑÐµÐ½Ð¸Ñ-Ñ Ð¾Ð·ÑÐ¸Ð½Ð° Ð´Ð»Ñ Ð¿Ð¾Ð´Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ñ ÐºÐ¾Ð»Ð»ÐµÐºÑÐ¸Ð¸ Ð³ÑÐ¸Ð±Ð¾Ð² Ð°ÑÐ±ÑÑÐºÑÐ»ÑÑÐ½Ð¾Ð¹ Ð¼Ð¸ÐºÐ¾ÑÐ¸Ð·Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Identification of the plant of the Labiatae family â a model host plant for maintaining a collection fungi of arbuscular mycorrhiza
Creators: name: ÐÐ°Ð»Ð¸Ð½Ð¸Ð½Ð°, ÐÐ½Ð°ÑÑÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Ð³ÑÐ¸Ð±Ñ Ð°ÑÐ±ÑÑÐºÑÐ»ÑÑÐ½Ð¾Ð¹ Ð¼Ð¸ÐºÐ¾ÑÐ¸Ð·Ñ
Ð Ð¸Ð·Ð¾ÑÐ°Ð³ÑÑ Ð¸ÑÑÐµÐ³ÑÐ»ÑÑÐ¸Ñ
ÐÐ»ÐµÐºÑÑÐ°Ð½ÑÑÑ
Ð¼Ð¸ÐºÐ¾ÑÐ¸Ð·Ð°ÑÐ¸Ñ
Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾-Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ°Ñ Ð¸Ð´ÐµÐ½ÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ñ
Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð½Ð°Ñ ÑÐµÐ¿Ð½Ð°Ñ ÑÐµÐ°ÐºÑÐ¸Ñ (ÐÐ¦Ð )
arbuscular mycorrhiza fungi
Rhizophagus irregulares
Plectrantus
mycorrhization
molecular genetic identification
polymerase chain reaction (PCR)

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description: The purpose of the work is to identificate of a plant of the Labiatae family â a model host plant for maintaining a collection of arbuscular mycorrhiza fungi.Tasks:1) Conduct a literary and patent review on the subject;2) To master the technique of replanting isolates of AM-fungi, inoculation of the digestive system, cultivation of plant-fungal material for research and maintenance of the collection of the institute;3) To obtain data from sequencing results using the Illumina MiSeq device of soil samples of roots of a plant of the clear-flowered family (presumed species Plectranthus australis R.Br .).;4) To collect plant material for molecular genetic studies, master the technique, adapt and, using techniques with modifications, isolate DNA from the leaves of a plant sample; amplify plant DNA, visualize fragments of amplified DNA on gel electrophoresis, purify amplicons on silica gel; perform Sanger sequencing on ITS sequences, analyze the data obtained by bioinformatic methods using the Genbank database;5) Repeat the genetic identification of the plant using other molecular markers (ribosomal genes of the chloroplast genome), identify the frequency and spectrum of mutations in these areas in the studied specimen, using molecular phylogeny methods to present possible phylogenetic relationships within the genus Plectranthus, draw conclusions from the results obtained;6) To collect plant material at the flowering phase, to carry out a morphological assessment to the type of plant sample with the help of a flower, to draw a conclusion about the species of the host plant.In the course of the research, the molecular genetic identification of the host plant used to maintain a collection of various strains of arbuscular mycorrhiza was carried out. It was determined that the studied plant belongs to a new virtual taxon according to various molecular brands, however, it is difficult to accurately identify the type of plant at this stage of research and requires detailed morphological studies of the generative organ.