Additional file 2: Table S1a. of Pro-survival responses to the dual inhibition of anti-apoptotic Bcl-2 family proteins and mTOR-mediated signaling in hypoxic colorectal carcinoma cells
Creators: name: Risberg, Karianne
name: Redalen, Kathrine
name: Sønstevold, Linda
name: Bjørnetrø, Tonje
name: Sølvernes, Janne
name: Ree, Anne
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biochemistry
subject: Cell Biology
subject: Molecular Biology
subject: Pharmacology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: FOS: Chemical sciences
subject: Developmental Biology
subject: Cancer
subject: Hematology
subject: 60506 Virology
subject: FOS: Biological sciences
subject: FOS: Biological sciences

description: Cell viability of normoxic colorectal carcinoma cell lines. From at least three independent experiments, each at least with triplicate setups, mean ± SD cell viability values were calculated in percentage of values from the corresponding controls (vehicle-treated cells). Treatments (72-h incubations): ABT-737 (inhibitor of anti-apoptotic Bcl-2 family proteins; 10 μM), AZD8055 (mTOR inhibitor; 0.10 μM), both compounds in combination. Difference in values from cells given AZD8055 or the combination treatment was compared by two-tailed Student’s t-test. Mutation status is indicated for each cell line. Table S1b. Cell viability of hypoxic colorectal carcinoma cell lines. From at least three independent experiments, each at least with triplicate setups, mean ± SD cell viability values were calculated in percentage of values from the corresponding controls (vehicle-treated cells). Treatments (72-h incubations): ABT-737 (inhibitor of anti-apoptotic Bcl-2 family proteins; 10 μM), AZD8055 (mTOR inhibitor; 0.10 μM), both compounds in combination. Difference in values from cells given AZD8055 or the combination treatment was compared by two-tailed Student’s t-test. Mutation status is indicated for each cell line. Table S2. Cell viability of normoxic colorectal carcinoma cell lines. From between three and seven independent experiments, each at least with triplicate setups, mean ± SD cell viability values were calculated in percentage of values from the corresponding controls (vehicle-treated cells). Treatments (24-h incubations): ABT-737 (inhibitor of anti-apoptotic Bcl-2 family proteins; 10 μM), AZD8055 (mTOR inhibitor; 10 μM), both compounds in combination (combo-Rx). Difference in values from cells given AZD8055 or combo-Rx was compared by two-tailed Student’s t-test. Mutation status is indicated for each cell line. Table S3a. Cell viability of normoxic colorectal carcinoma cell lines. From at least three independent experiments, each at least with triplicate setups, mean ± SD cell viability values were calculated in percentage of values from the corresponding controls (vehicle-treated cells). Treatments (24-h incubations): ABT-737 (inhibitor of anti-apoptotic Bcl-2 family proteins; 10 μM), BEZ235 (PI3K/mTOR inhibitor; 0.10 μM), both compounds in combination. Difference in values from cells given BEZ235 or the combination treatment was compared by two-tailed Student’s t-test. Mutation status is indicated for each cell line. Table S3b. Cell viability of hypoxic colorectal carcinoma cell lines. From at least three independent experiments, each at least with triplicate setups, mean ± SD cell viability values were calculated in percentage of values from the corresponding controls (vehicle-treated cells). Treatments (24-h incubations): ABT-737 (inhibitor of anti-apoptotic Bcl-2 family proteins; 10 μM), BEZ235 (PI3K/mTOR inhibitor; 0.10 μM), both compounds in combination. Difference in values from cells given BEZ235 or the combination treatment was compared by two-tailed Student’s t-test. Mutation status is indicated for each cell line. (DOCX 36 kb)