title: Ð£ÑÐ¸Ð»ÐµÐ½Ð¸Ðµ Ð¿Ð¾ÑÐ¾Ð¾Ð±ÑÐ°Ð·ÑÑÑÐµÐ¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð°Ð½ÑÐ¸Ð¼Ð¸ÐºÑÐ¾Ð±Ð½ÑÑ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð² Ð¿ÑÐ¸ Ð²Ð²ÐµÐ´ÐµÐ½Ð¸Ð¸ ÑÐ»Ð°Ð²Ð¾Ð½Ð¾Ð¸Ð´Ð¾Ð²
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Enhancement of the pore-forming activity of antimicrobial peptides with the introduction of flavonoids
Creators: name: ÐÐ°ÑÑÑÐ½ÑÐº, ÐÐµÑÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð»Ð¸Ð¿Ð¸Ð´Ð½ÑÐµ Ð±Ð¸ÑÐ»Ð¾Ð¸
ÑÐ»Ð°Ð²Ð¾Ð½Ð¾Ð¸Ð´Ñ
Ð½Ð¸Ð·Ð¸Ð½
Ð¿Ð¾Ð»Ð¸Ð¼Ð¸ÐºÑÐ¸Ð½
Ð³ÑÐ°Ð½Ð¸ÑÐ½ÑÐ¹ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð» Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ñ
ÑÐ°Ð·Ð¾Ð²ÑÐ¹ Ð¿ÐµÑÐµÑ Ð¾Ð´ Ð»Ð¸Ð¿Ð¸Ð´Ð¾Ð²
lipid bilayers
flavonoids
nisin
polymyxin
membrane boundary potential
lipid phase transition

description: ÐÐ°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÐµ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸ Ð¸ Ð¾Ð½ÐºÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ñ Ð¾ÑÑÐ°ÑÑÑÑ Ð¾ÑÑÑÐ¾Ð¹ Ð¿ÑÐ¾Ð±Ð»ÐµÐ¼Ð¾Ð¹ Ð´Ð»Ñ Ð·Ð´ÑÐ°Ð²Ð¾Ð¾ÑÑÐ°Ð½ÐµÐ½Ð¸Ñ Ð¸Ð·-Ð·Ð° ÑÐ¾ÑÑÐ° ÑÐ»ÑÑÐ°ÐµÐ² Ð»ÐµÐºÐ°ÑÑÑÐ²ÐµÐ½Ð½Ð¾Ð¹ ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾ÑÑÐ¸. ÐÐ½ÑÐ¸Ð±Ð¸Ð¾ÑÐ¸Ðº Ð¿Ð¾Ð»Ð¸Ð¼Ð¸ÐºÑÐ¸Ð½ Ð ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸Ð·ÑÐµÑÑÑ ÑÐ¸ÑÐ¾ÐºÐ¸Ð¼ ÑÐ¿ÐµÐºÑÑÐ¾Ð¼ Ð¸ Ð½Ð¸Ð·ÐºÐ¸Ð¼ ÑÐ¸ÑÐºÐ¾Ð¼ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ñ ÑÐµÐ·Ð¸ÑÑÐµÐ½ÑÐ½Ð¾ÑÑÐ¸, Ð½Ð¾ Ð¾Ð±Ð»Ð°Ð´Ð°ÐµÑ Ð²ÑÑÐ¾ÐºÐ¾Ð¹ ÑÐ¾ÐºÑÐ¸ÑÐ½Ð¾ÑÑÑÑ. ÐÐ°Ð½ÑÐ¸Ð±Ð¸Ð¾ÑÐ¸Ðº Ð½Ð¸Ð·Ð¸Ð½ Ð¸Ð¼ÐµÐµÑ Ð¼Ð½Ð¾Ð³Ð¾Ð¾Ð±ÐµÑÐ°ÑÑÐ¸Ð¹ Ð¿ÑÐ¾ÑÐ¸Ð²Ð¾ÑÐ°ÐºÐ¾Ð²ÑÐ¹ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð». ÐÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð¾Ð¼ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ð¾Ð±Ð¾Ð¸Ñ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð² ÑÐ²Ð»ÑÐµÑÑÑ Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¿Ð¾Ñ Ð² Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ð°Ñ ÐºÐ»ÐµÑÐ¾Ðº-Ð¼Ð¸ÑÐµÐ½ÐµÐ¹. Ð ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°ÑÐ¾ÑÐ¾Ð² Ð¿Ð¾ÑÐ¾Ð¾Ð±ÑÐ°Ð·ÑÑÑÐµÐ¹, Ð° ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾, ÑÐµÑÐ°Ð¿ÐµÐ²ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð´Ð°Ð½Ð½ÑÑ Ð°Ð³ÐµÐ½ÑÐ¾Ð² Ð¼Ð¾Ð¶Ð½Ð¾ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°ÑÑ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÐµ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¾ÑÑ ÑÐ¸Ð·Ð¸ÐºÐ¾-ÑÐ¸Ð¼Ð¸ÑÐµÑÐºÐ¸Ñ ÑÐ²Ð¾Ð¹ÑÑÐ² Ð¼ÐµÐ¼Ð±ÑÐ°Ð½. Ð¦ÐµÐ»ÑÑ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÐµÑÑÑ Ð¿Ð¾Ð¸ÑÐº ÑÐ¾ÐµÐ´Ð¸Ð½ÐµÐ½Ð¸Ð¹, ÑÐ¿Ð¾ÑÐ¾Ð±Ð½ÑÑ ÑÑÐ¸Ð»Ð¸Ð²Ð°ÑÑ Ð¿Ð¾ÑÐ¾Ð¾Ð±ÑÐ°Ð·ÑÑÑÑÑ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð½Ð¸Ð·Ð¸Ð½Ð° Ð¸ Ð¿Ð¾Ð»Ð¸Ð¼Ð¸ÐºÑÐ¸Ð½Ð° Ð, ÑÑÐµÐ´Ð¸ ÑÐ»Ð°Ð²Ð¾Ð½Ð¾Ð¸Ð´Ð¾Ð². ÐÑÐ»Ð¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ñ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ Ð¸Ð·Ð¼ÐµÑÐµÐ½Ð¸Ñ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð³ÑÐ°Ð½Ð¸ÑÐ½Ð¾Ð³Ð¾ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð° Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ñ Ð¿ÑÐ¸ Ð²Ð²ÐµÐ´ÐµÐ½Ð¸Ð¸ ÑÐ»Ð°Ð²Ð¾Ð½Ð¾Ð¸Ð´Ð¾Ð², Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð¾ÑÐµÐ½ÐºÐ° Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÑ Ð¸ ÐºÐ¾Ð¾Ð¿ÐµÑÐ°ÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÑÐ°Ð·Ð¾Ð²Ð¾Ð³Ð¾ Ð¿ÐµÑÐµÑÐ¾Ð´Ð° Ð»Ð¸Ð¿Ð¸Ð´Ð¾Ð² Ð² Ð¿ÑÐ¸ÑÑÑÑÑÐ²Ð¸Ð¸ ÑÐµÑÑÐ¸ÑÑÐµÐ¼ÑÑ ÑÐ»Ð°Ð²Ð¾Ð½Ð¾Ð¸Ð´Ð¾Ð². Ð ÑÐ°ÐºÐ¶Ðµ Ð±ÑÐ»Ð° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð¾ÑÐµÐ½ÐºÐ° Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð½Ð¸Ð·Ð¸Ð½- Ð¸ Ð¿Ð¾Ð»Ð¸Ð¼Ð¸ÐºÑÐ¸Ð½- Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð¹ Ð¿ÑÐ¾Ð½Ð¸ÑÐ°ÐµÐ¼Ð¾ÑÑÐ¸ Ð»Ð¸Ð¿Ð¸Ð´Ð½Ð¾Ð³Ð¾ Ð±Ð¸ÑÐ»Ð¾Ñ Ð¿Ð¾Ð´ Ð´ÐµÐ¹ÑÑÐ²Ð¸ÐµÐ¼ Ð»ÑÑÐµÐ¾Ð»Ð¸Ð½Ð°, ÑÐ¸ÑÐµÑÐ¸Ð½Ð°, Ð±ÑÑÐµÐ¸Ð½Ð°, ÐºÐ²ÐµÑÑÐµÑÐ¸Ð½Ð°, Ð¼Ð¸ÑÐ¸ÑÐµÑÐ¸Ð½Ð° Ð¸ ÑÐµÑÐ²ÐµÑÐ°ÑÑÐ¾Ð»Ð°. Ð ÑÐ°Ð±Ð¾ÑÐµ ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¾, ÑÑÐ¾ Ð»ÑÑÐµÐ¾Ð»Ð¸Ð½ Ð¸ ÑÐ¸ÑÐµÑÐ¸Ð½ Ð²ÑÐ·ÑÐ²Ð°ÑÑ Ð·Ð½Ð°ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ðµ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ðµ Ð³ÑÐ°Ð½Ð¸ÑÐ½Ð¾Ð³Ð¾ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð° Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ñ; ÑÑÐµÐ´Ð¸ ÑÐ»Ð°Ð²Ð¾Ð½Ð¾Ð¸Ð´Ð¾Ð² Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½ Ð°Ð³ÐµÐ½Ñ, ÑÐ¿Ð¾ÑÐ¾Ð±Ð½ÑÐ¹ Ð¿Ð¾Ð²ÑÑÐ°ÑÑ Ð³ÑÐ°Ð½Ð¸ÑÐ½ÑÐ¹ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð» Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ñ, Ð±Ð°Ð¹ÐºÐ°Ð»ÐµÐ¸Ð½; Ð»ÑÑÐµÐ¾Ð»Ð¸Ð½, ÑÐ¸ÑÐµÑÐ¸Ð½ Ð¸ ÑÑÐ¸Ð·Ð¸Ð½ Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÑÑ ÑÐ°Ð·ÑÐ¿Ð¾ÑÑÐ´Ð¾ÑÐ¸Ð²Ð°ÑÑÐµÐµ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ðµ Ð½Ð° ÑÐ¾ÑÑÐ¾Ð»Ð¸Ð¿Ð¸Ð´Ð½ÑÐµ Ð±Ð¸ÑÐ»Ð¾Ð¸; Ð²ÑÑÐ²Ð»ÐµÐ½Ð¾, ÑÑÐ¾ ÑÐ¸ÑÐµÑÐ¸Ð½, Ð»ÑÑÐµÐ¾Ð»Ð¸Ð½, ÐºÐ²ÐµÑÑÐµÑÐ¸Ð½ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ñ Ð¿Ð¾ÑÐµÐ½ÑÐ¸ÑÐ¾Ð²Ð°ÑÑ Ð¿Ð¾ÑÐ¾Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¿Ð¾Ð»Ð¸Ð¼Ð¸ÐºÑÐ¸Ð½Ð° Ð Ð¸ Ð½Ð¸Ð·Ð¸Ð½Ð°.
description: Bacterial infections and oncological diseases remain an acute problem for public health due to the increase in cases of drug resistance. The antibiotic polymyxin B is characterized by a wide spectrum and low risk of resistance, but has a high toxicity. The lantibiotic nisin has a promising anti-cancer potential. The mechanism of action of both peptides is the formation of pores in the membranes of target cells. Various modifiers of the physico-chemical properties of membranes can be used as potentiators of the pore-forming and consequently therapeutic activity of the agents. The aim of this work is to search for compounds capable of enhancing the pore-forming activity of nisin and polymyxin B among flavonoids. Electrophysiological measurements of changes in the boundary potential of the membrane at the introduction of flavonoids were carried out, an assessment of the temperature and the cooperativeness of the lipid phase transition changes in the presence of the tested flavonoids was carried out. Changes in the nisin and polymyxin-induced permeability of the lipid bilayer under the action of luteolin, fisetin, butein, quercetin, myricetin and resveratrol were also evaluated. It was found that luteolin and fisetin caused a significant decrease in the membrane boundary potential. Baicalein was capable of increasing the membrane boundary potential. Luteolin, fisetin and chrysin had a disordering effect on phospholipid bilayers; it was revealed that fisetin, luteolin, quercetin were able to potentiate the pore formation of polymyxin B and nisin.