title: ÐÐ¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾-ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐµ Ð¼ÐµÑ Ð°Ð½Ð¸Ð·Ð¼Ñ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð½Ð¾Ð¹ ÑÐµÐ³ÑÐ»ÑÑÐ¸Ð¸ ÑÑÐ½ÐºÑÐ¸Ð¹ ÐºÐ¾Ð¶Ð¸ Ð¿ÑÐ¸ ÐµÑ ÑÑÐ°ÑÐµÐ½Ð¸Ð¸
title: Ð½Ð°ÑÑÐ½ÑÐ¹ Ð´Ð¾ÐºÐ»Ð°Ð´
titleType: Subtitle
title: Molecular and cellular mechanisms of peptide regulation of skin functions during its aging
Creators: name: ÐÑÐ·Ð½ÐµÑÐ¾Ð²Ð°, ÐÐºÐ°ÑÐµÑÐ¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2018
Subjects: ÐÐµÐ»ÐºÐ¸
ÐÐµÐ¿ÑÐ¸Ð´Ñ
ÑÑÐ°ÑÐµÐ½Ð¸Ðµ
ÐºÐ¾Ð¶Ð°
ÐÐÐ

description: ÐÐ°ÑÑÐ½ÑÐ¹ Ð´Ð¾ÐºÐ»Ð°Ð´ Ð¿Ð¾ÑÐ²ÑÑÐµÐ½ Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾-ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð¾Ð² Ð¿ÐµÐ¿ÑÐ¸Ð´Ð½Ð¾Ð¹ ÑÐµÐ³ÑÐ»ÑÑÐ¸Ð¸ ÑÑÐ½ÐºÑÐ¸Ð¹ ÐºÐ¾Ð¶Ð¸ Ð¿ÑÐ¸ ÐµÑ ÑÑÐ°ÑÐµÐ½Ð¸Ð¸. Ð ÑÐ°Ð±Ð¾ÑÐµ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÑ Ð±ÑÐ»Ð¸ ÑÐ°Ð·Ð´ÐµÐ»ÐµÐ½Ñ Ð½Ð° Ð´Ð²Ð° Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ ÑÐ°Ð·Ð´ÐµÐ»Ð°. Ð Ð¿ÐµÑÐ²Ð¾Ð¹ ÑÐ°ÑÑÐ¸ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð¸Ð¼Ð¼ÑÐ½Ð¾ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð¹ ÐºÐ¾Ð½ÑÐ¾ÐºÐ°Ð»ÑÐ½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð² KE, KED Ð¸ AED Ð½Ð° Ð¿ÑÐ¾ÑÐµÑÑÑ Ð¿ÑÐ¾Ð»Ð¸ÑÐµÑÐ°ÑÐ¸Ð¸ (Ki67), ÑÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸Ð¸ Ð¸ ÑÑÐ°ÑÐµÐ½Ð¸Ñ (CD98hc), ÑÐµÐ¼Ð¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¼ÐµÐ¶ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ Ð¼Ð°ÑÑÐ¸ÐºÑÐ° (MMP9) Ð¸ Ð°Ð¿Ð¾Ð¿ÑÐ¾Ð·Ð° (Caspase-3) Ð² ÐºÑÐ»ÑÑÑÑÐµ ÐºÐ»ÐµÑÐ¾Ðº ÑÐ¸Ð±ÑÐ¾Ð±Ð»Ð°ÑÑÐ¾Ð² ÐºÐ¾Ð¶Ð¸ Ð¿ÑÐ¸ Ð¸Ñ ÑÑÐ°ÑÐµÐ½Ð¸Ð¸. ÐÐ°Ð¶Ð´ÑÐ¹ Ð¸Ð· Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð² Ð¿Ð¾Ð²ÑÑÐ°Ð» ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð¼Ð¾Ð»ÐµÐºÑÐ» Ki67 Ð¸ CD98hc, ÑÐ¸Ð½ÑÐµÐ· ÐºÐ¾ÑÐ¾ÑÑÑ ÑÐ½Ð¸Ð¶Ð°ÐµÑÑÑ Ð¿ÑÐ¸ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¼ ÑÑÐ°ÑÐµÐ½Ð¸Ð¸, Ð¸ ÑÐ½Ð¸Ð¶Ð°Ð» Ð²Ð¾Ð·ÑÐ°ÑÑÐ°ÑÑÐ¸Ð¹ Ð¿ÑÐ¸ ÑÑÐ°ÑÐµÐ½Ð¸Ð¸ ÑÐ¸Ð½ÑÐµÐ· ÐÐÐ 9. ÐÐµÐ¿ÑÐ¸Ð´ AED ÑÐ°ÐºÐ¶Ðµ Ð¿Ð¾Ð½Ð¸Ð¶Ð°Ð» Ð¸Ð½ÑÐµÐ½ÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÐºÐ°ÑÐ¿Ð°Ð·Ð°-Ð·Ð°Ð²Ð¸ÑÐ¼Ð¾Ð³Ð¾ Ð°Ð¿Ð¾Ð¿ÑÐ¾Ð·Ð°, Ð²Ð¾Ð·ÑÐ°ÑÑÐ°ÑÑÐ¸Ð¹ Ð¿ÑÐ¸ ÑÑÐ°ÑÐµÐ½Ð¸Ð¸ ÐºÑÐ»ÑÑÑÑ ÐºÐ»ÐµÑÐ¾Ðº. 
ÐÑÐ¾ÑÐ°Ñ ÑÐ°ÑÑÑ ÑÐ°Ð±Ð¾ÑÑ Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾ÑÑÐ¸ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð² KE, KED Ð¸ AED Ð½Ð° ÑÑÑÑÐºÑÑÑÑ Ð¼Ð¾Ð»ÐµÐºÑÐ»Ñ ÐÐÐ Ð² ÑÐ°ÑÑÐ²Ð¾ÑÐµ. ÐÐµÑÐ¾Ð´Ð¾Ð¼ ÑÐ¿ÐµÐºÑÑÐ¾ÑÐ¾ÑÐ¾Ð¼ÐµÑÑÐ¸Ð¸ Ð² ÑÐ»ÑÑÑÐ°ÑÐ¸Ð¾Ð»ÐµÑÐ¾Ð²Ð¾Ð¹ Ð¾Ð±Ð»Ð°ÑÑÐ¸ Ð±ÑÐ»Ð¾ ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¾ Ð¾ÑÑÑÑÑÑÐ²Ð¸Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼ÑÑ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð² Ð½Ð° Ð²ÑÐ¾ÑÐ¸ÑÐ½ÑÑ ÑÑÑÑÐºÑÑÑÑ ÐÐÐ. ÐÐµÑÐ¾Ð´Ð¾Ð¼ Ð²Ð¸ÑÐºÐ¾Ð·Ð¸Ð¼ÐµÑÑÐ¸Ð¸ Ð±ÑÐ»Ð¾ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¾ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸Ðº ÑÑÐµÑÐ¸ÑÐ½Ð¾Ð¹ ÑÑÑÑÐºÑÑÑÑ Ð¼Ð¾Ð»ÐµÐºÑÐ»Ñ ÐÐÐ Ð² ÑÐ°ÑÑÐ²Ð¾ÑÐµ Ð¿ÑÐ¸ Ð´Ð¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸Ð¸ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð² KED Ð¸ AED.
description: Molecular and cellular mechanisms of peptide regulation of skin functions during its aging have been researched. The experiments have been divided into two logical parts. In the first part the influence of the KE, KED, AED peptides on the processes of proliferation (Ki67), regeneration and aging (CD98hc), extracellular matrix remodeling (MMP9), and apoptosis (Caspase-3) in the skin fibroblasts culture during its aging has been researched using the method of confocal microscopy. Each of the peptides increased expression of the Ki67 and CD98hc molecules, synthesis of which lowers during cells aging, and decreased synthesis of MMP9, which rises during aging. AED peptide also lowered intensity of the caspase dependent apoptosis, which increases during the cells culture aging.
The second part of the work describes the research of the possibility of KE, KED, AED peptides to influence DNA structure in solution. The lack of influence of the researched peptides on the secondary DNA structure has been found using a method of spectrophotometery in the ultraviolet interval. Changes in the characteristics of the tertiary DNA structure in solution after the addition of KED and AED peptides has been found using a method of viscosimetry.