THE ORAL MUCOSA REACTION ON THE SUTURE MATERIALS AND THE COMBINED METHOD OF CONNECTING SURGICAL WOUNDS IN THE EXPERIMENT
Creators: name: Pavlenko, O.
name: Boiko, M.
name: Savitskaya, I.
name: Geylenko, O.
Publisher: name: The Scientific Heritage
Year: 2021
Subjects: oral mucosa
oral surgery
polyester
adhesive composition
nylon
wound healing
morphometry
histology.

description: Introduction. Hermetic connection of wound edges and ensuring quality hemostasis is the final stage of any surgery. The connection of tissues and the influence of connecting elements on them, including in oral surgery, is an urgent problem of modern dentistry. Despite the invention of new methods of connection and modification of existing ones, there is no consensus of experts on their usage in each clinical situation. The suture technique is one of a choice. The stage of selection suture material is one of the main points in surgical treatment, on which depends the course of the postoperative period and the timing of wound healing. Due to the ambiguous views of specialists, as an alternative to suturing techniques, a medical adhesive composition was created, which facilitated the surgeons work. However, given the ambiguity of views on the usage of each of the methods, this issue remains debatable to this day. Objectives. Based on the assessment of histological material and analysis of morphometric changes in the oral mucosa (OM) to investigate and study the course of regeneration processes after suturing 5/0 based on polyester, 5/0 polyester in combination with medical adhesive composition and 5/0 nylon. Material and methods. The studies were performed on 76 adult outbred rabbits of different sexes with a body weight of 2, 6 to 4, 2 kg due to the similarity of the histological structure of the mucous membrane with the human. A series of incisions of the soft tissues of the vestibulum of the oral cavity of the maxilla were made. Rabbits were divided into 3 groups. In group 1, the joints of wound edges were made by applying knotted sutures made of 5/0 polyester. In group 2, a combined method of joining wound edges was performed by applying 5/0 polyester sutures and using the medical adhesive composition "Histoacryl". In group 3, oral mucosa connections were performed by applying knotted sutures made of nylon 5/0. On days 3, 7, 14 and 21, the animals were removed from the experiment. Tissue samples in the affected area were removed so that the wound canal with a deviation of 3 mm to healthy tissues fell within its boundaries, histological examination of the taken material was performed. Results. In the course of the study, 45 calculations of the U-test of Mann-Whitney were performed. It was found that in 38 cases there are significant differences in the levels of morphometric parameters. Analysis of the dynamics of the average values studied during the experiment reveals that in rabbits of group 1 (polyester 5/0) they were all recorded at the highest level. In rabbits of group 2 (polyester 5/0 in combination with the adhesive composition) there was a significant difference in quantitative indicators for each of the days in comparison with groups 1 and 3, faster sutures erruption, moderate inflammatory-cellular reaction of tissues. The lowest values at all terms of observation were observed in group 3 (nylon 5/0), the lowest inflammatory-cellular reaction of tissues. Conclusions. The high reliability of differences in the levels of morphometric parameters between groups of rabbits on each of the days was revealed, as evidenced by the calculated values of the U-Mann-Whitney test. In 37 cases, which is 97.4%, the significance level was p≤0.01, in one case p≤0.05. Rabbits of group 3 had the lowest values in all indicators, a decrease in the levels of all studied cells from day 7 was recorded, which indicates a lower inflammatory-cellular response of oral mucosa and a reduction in the time of postoperative wound healing.