title: Ð¡ÑÐ°Ð²Ð½ÐµÐ½Ð¸Ðµ Ð¸ Ð¾Ð¿ÑÐ¸Ð¼Ð¸Ð·Ð°ÑÐ¸Ñ ÑÐ»ÐµÐºÑÑÐ¾Ñ Ð¸Ð¼Ð¸ÑÐµÑÐºÐ¸Ñ Ð¸ Ð¾Ð¿ÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÐµÑ Ð½Ð¾Ð»Ð¾Ð³Ð¸Ð¹ Ð´Ð»Ñ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÑÐµÑÑÐ»Ð¾Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð½Ð°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Comparison and optimization of electrochemical and optical technologies for quantitative analysis of ceruloplasmin activity
Creators: name: ÐÑÐ°ÑÐ½Ð¾Ð¿ÐµÐµÐ², ÐÐ¸ÑÐ¸Ð»Ð»
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: ÑÐµÑÑÐ»Ð¾Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð½
Ð¾ÐºÑÐ¸Ð´Ð°Ð·Ð½Ð°Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ
ÑÐ¿ÐµÐºÑÑÐ¾ÑÐ¾ÑÐ¾Ð¼ÐµÑÑÐ¸ÑÐµÑÐºÐ¸Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ
Ð°Ð¼Ð¿ÐµÑÐ¾Ð¼ÐµÑÑÐ¸ÑÐµÑÐºÐ¸Ðµ Ð¸Ð·Ð¼ÐµÑÐµÐ½Ð¸Ñ
ÑÐµÑÑÐ¾ÐºÑÐ¸Ð´Ð°Ð·Ð°
ÑÐ»ÐµÐºÑÑÐ¾Ð´ ÐºÐ»Ð°ÑÐºÐ°
ceruloplasmin
oxidase activity
spectrophotometric studies
amperometric measurements
ferroxidase
clark electrode

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description: Object of study â Ceruloplasmin in blood serum. Purpose of the work â Optimization of measurement techniques for investigating the oxidase activity level of blood serum in laboratory animals. Research results: During the study, measurement techniques for the oxidative activity of ceruloplasmin (Cp) in rat blood serum were optimized. The research was conducted using spectrophotometric and electrochemical methods. The spectrophotometric method involved measuring the kinetics of o-dianisidine oxidation by oxygen, catalyzed by Cp, under various conditions (pH of the buffer solution, temperature, volume of substrate, and serum). The electrochemical method allowed measurement of ferroxidase Cp activity in serum samples. The obtained data were analyzed using regression analysis, enabling the determination of the specific activity of Cp under various combinations of factors. Novelty of the work lies in comparing two different methods for measuring ceruloplasmin activity and determining the optimal conditions for their application in lab studies and in clinical practice. This approach improves the accuracy of diagnosing diseases related to copper metabolism and proposes new methodologies for studying biochemical parameters of blood serum. The applied software included the control software of Specord-M400 spectrometer and the Econix potentiostat, along with a spreadsheet processor.