Supplementary data to: A previously unknown feeding mode in millipedes and the convergence of fluid feeding across arthropods
Creators: name: Moritz, Leif
name: Borisova, Elena
name: Hammel, Jörg
name: Blanke, Alexander
name: Wesener, Thomas
Publisher: name: Zenodo
Year: 2021
Subjects: Myriapoda, Diplopoda, Colobognatha, Polyzoniida, Siphonocryptida, Siphonophorida

description: <strong>Supplementary material to: A previously unknown feeding mode in millipedes and the convergence of fluid feeding across arthropods</strong> <strong>Article:</strong> Moritz, L., Brisova, E., Hammel, J.U., Blanke, A., Wesener, T. 2022. A previously unknown feeding mode in millipedes and the convergence of fluid feeding across arthropods. <em>Science Advances</em> 8, eabm0577 <br> https://www.science.org/doi/10.1126/sciadv.abm0577 The zip folder contains image stacks of histological sections and SR-µCT data. <strong>Histologcial section</strong> Specimen were fixed in Bouin solution and embedded in in epoxy resin (Araldite CY212, Agar Scientific Ltd., R1030). Sections (1 µm) were obtained with a Leica HistoCore NANOCUT R microtome with a DiATOME histo Jumbo diamond blade and stained with 1% Toluidine blue (PanReac AppliChem A3842.0010) for 2 minutes. Sections were Photographed with an Olympus BX61VS light-microscope equipped with a VS120-S6-W slide loader system. Pixel size is 0.1724000 µm. Histological sections are deposited at the Zoological Research Museum Alexander Koenig (ZFMK-HIST000002 (<em>H. canariensis</em>, female, cross sections), ZFMK-HIST000003 (<em>H. canariensis</em>, male, sagittal sections)). Image Stack can be opened in Fiji/ImageJ by drag and drop. <strong>Hirudicryptus-canariensis_HIST0002:</strong> Cross sections of:<em> Hirudicryptus canariensis </em>(Loksa, 1967), female, SPAIN, Canary Islands, Tenerife, Anaga Mountains, Along hiking trail undernetah Road TF-12 close to junction to Las Carboneras, 28°32'23.26''N 16°16'01.76''W, 805m, leg. L. Moritz &amp; B. Wipfler, 13.x.2019. <strong>Hirdudicryptus-canariensis_male_HIST00003:</strong> Sagittal sections of:<em> Hirudicryptus canariensis</em> (Loksa, 1967), male, SPAIN, Canary Islands, Tenerife, Anaga Mountains, Along hiking trail undernetah Road TF-12 close to junction to Las Carboneras, 28°32'23.26''N 16°16'01.76''W, 805m, leg. L. Moritz &amp; B. Wipfler, 13.x.2019. <strong>Synchrotron micro-computed tomography (SR-µCT)</strong> SR-µCT data has been obtained at DESY (Hamburg, Germany), PSI (Villigen, Switzerland) and SPring8 (Hyogo, Japan). Image Stacks have been cropped, and brigthness and contrast has been adjusten in Fiji/ImageJ 1.53c (https://imagej.net/software/fiji/). Segmentation has been done in ITK-SNAP 3.8.0 (http://www.itksnap.org/pmwiki/pmwiki.php). Image stacks (.tiff &amp; .hdr) can be opened in ImageJ and ITK-SNAP by drag and drop. Segmentation (.gz) can be loaded in ITK-SNAP by drag and drop. <strong>Hirudicryptus-canariensis_MYR0478_SR-CT</strong>: <em>Hirudicryptus canariensis </em>(Loksa, 1967), SPAIN, Canary Islands, Tenerife, Anaga mountains. SR-µCT data obtained at SPring8, voxel-size = 0.2159 µm. <strong>Hirudisoma-roseum_MYR11195_SR-CT:</strong> <em>Hirudisoma roseum</em> (Victor, 1839), female, GEORGIA, Adscharien, Kobuleti District, Kintrishi National Park, at bisection behind ranger station, 41°43'49.17"N, 41°58'54.23"E, 450m, 20.vii.2018. SR-µCT data obtained at DESY, voxel-size = 1.2 µm. <strong>Polyzonium-germanicum_MYR11196_SR-CT:</strong> <em>Polyzonium germanicum</em> Brandt, 1837, female, GERMANY, Dresdner Heide, 51°05'57''N 13°47'07''E, alt. 159m, Südost Hang mit Laubwald, unter Stück Kiefernstamm am Boden neben Waldweg, 24.08.2019. SR-µCT data obtained at DESY, voxel-size = 1.2157 µm. <strong>Rhinotus-purpureus_MYR11197_SR-CT</strong>: <em>Rhinotus purpureus</em> (Pocock, 1894), female, GERMANY, Bonn, Botanical garden, Victoria Haus, leg. L. Moritz,15.iv.2020 . SR-µCT data obtained at PSI, voxel-size = 0.3 µm <strong>Siphonophora-sp_F8_SR-CT:</strong><em> Siphonophora</em> sp., female. SR-µCT data obtained at SPring8, voxel-size = 0.4422166 µm. <strong>Siphonophora-sp_INDOSYMBIOSYS-CCDB26227-E04_SR-CT:</strong><em>Siphonophora</em> sp., female, INDONESIA, Java, Jawa Barat, Cidhau, 6,72779601998627S, 106,713530011475E, 1452 m, Main forest, upper part, leg. Myriapoda Team, 23.ix.2015. SR-µCT data obtained at DESY, voxel-size = 1.2 µm <strong>Siphonorhinus-sp_F9_SR-CT:</strong> <em>Sphonorhinus</em> sp., female. SR-µCT data obtained at SPring8, voxel-size = 0.66 µm.
description: SR-CT data have been acquired at the Deutsche Elektronen Synchrotron (DESY; beamtimes I-20170869, I-20180474, and BAG-20190010), at the Paul Scherrer Institute (PSI; beamtime 20171469), and at the SPring-8 (Hyogo, Japan). Funding: This work received support from the German Research Foundation (DFG; Projects WE 2479/4-1 and BL 1355/5-1), the European Research Council (ERC; grant agreement no. 754290), and the NSF (grants DEB 97-12438 and 05-29715)