Pestalotiopsis rhaphiolepidis C. Z. Yin, Z. X. Zhang & X. G. Zhang 2024, sp. nov.
Creators: name: Yin, Changzhun
name: Zhang, Zhaoxue
name: Wang, Shi
name: Ma, Liguo
name: Zhang, Xiuguo
Publisher: name: Zenodo
Year: 2024
Subjects: Biodiversity
Taxonomy
Fungi
Ascomycota
Sordariomycetes
Amphisphaeriales
Sporocadaceae
Pestalotiopsis
Pestalotiopsis rhaphiolepidis

description: Pestalotiopsis rhaphiolepidis C. Z. Yin, Z. X. Zhang & X. G. Zhang sp. nov.
Fig. 4
Type.
China, Hainan Province, Jianfeng Town (18 ° 42 ' 35 " N, 108 ° 52 ' 35 " E), from diseased leaves of Rhaphiolepis indica, 11 Apr 2023, C. Z. Yin, Z. X. Zhang and X. G. Zhang, holotype HMAS 352669, ex-type living culture SAUCC 367701.
Etymology.
Referring to the name of the host plant Rhaphiolepis indica.
Description.
Conidiomata in culture on PDA, 600–1000 µm diam, globular, solitary, black conidial masses permeated above the mycelium. Conidiophores mostly degenerated into conidiogenous cells, simple, hyaline. Conidiogenous cells fusiform, rough, discrete, 9.8–17.1 × 2.4–3.3 μm. Conidia fusiform, straight or slightly curved, 4 - septate, 18.0–23.1 × 3.8–5.1 μm; basal cell conical, hyaline, rough, thin-walled, 3.3–5.1 µm; three median cells subcylindrical, light brown or brown, rough, thick-walled, the first median cell from base 3.0–4.7 μm, the second median cell 3.4–5.3 μm, the third median cell 3.7–5.6 μm, together 10.1–15.6 μm; apical cell subcylindrical or conical, hyaline, smooth, thin-walled, 2.8–4.7 µm; basal appendage tubular, single, centric, straight or slightly bent, unbranched, 4.7–9.8 µm; apical appendages tubular, 2–3, straight or bent, unbranched, 5.2–18.5 μm. Sexual morph not observed.
Culture characteristics.
After 7 days of dark cultivation at 25 ° C on PDA, the colony diameter reached 90 mm, and the growth rate is 11.8–13.5 mm / day. Colonies filamentous to circular, flat, center raised, aerial mycelium on surface, with irregular edges, white, medium dense, fruiting bodies black; reverse white, multilayer rings from the middle to the edge.
Additional specimen examined.
China, Hainan Province, Jianfeng Town, from diseased leaves of Rhaphiolepis indica, 11 Apr 2023, C. Z. Yin, Z. X. Zhang and X. G. Zhang, living culture SAUCC 367702.
Notes.
According to phylogenetic trees based on ITS, tub 2 and tef 1 α, Pestalotiopsis rhaphiolepidis sp. nov. was closely related to P. inflexa in a well support branch (ML / BI = 98 / 1). P. rhaphiolepidis was different from P. inflexa by 9 / 508 bp in ITS, 30 / 529 bp in tub 2, and 16 / 465 bp in tef 1 α. Morphologically, P. rhaphiolepidis was different from P. inflexa by having shorter and thinner conidia (P. rhaphiolepidis: 18.0–23.1 × 3.8–5.1 vs. P. inflexa: 24.0–31.0 × 6.0–9.0 µm) and shorter apical appendages (P. rhaphiolepidis: 5.2–18.5 vs. P. inflexa: 20.0–30.0 μm) (Maharachchikumbura et al. 2011). Therefore, Pestalotiopsis rhaphiolepidis was identified as a new species of Pestalotiopsis by morphological and phylogenetic comparison.
description: Published as part of Yin, Changzhun, Zhang, Zhaoxue, Wang, Shi, Ma, Liguo & Zhang, Xiuguo, 2024, Three new species of Pestalotiopsis (Amphisphaeriales, Sporocadaceae) were identified by morphology and multigene phylogeny from Hainan and Yunnan, China, pp. 51-74 in MycoKeys 107 on pages 51-74, DOI: 10.3897/mycokeys.107.122026