Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines
Creators: name: Thery, Clotilde
name: Witwer, Kenneth W
name: Aikawa, Elena
name: Alcaraz, Maria Jose
name: Anderson, Johnathon D
name: Andriantsitohaina, Ramaroson
name: Antoniou, Anna
name: Arab, Tanina
name: Archer, Fabienne
name: Atkin-Smith, Georgia
name: Ayre, D Craig
name: Bach, Jean-Marie
name: Bachurski, Daniel
name: Baharvand, Hossein
name: Balaj, Leonora
name: Baldacchino, Shawn
name: Bauer, Natalie N
name: Baxter, Amy
name: Bebawy, Mary
name: Beckham, Carla
name: Zavec, Apolonija Bedina
name: Benmoussa, Abderrahim
name: Berardi, Anna C
name: Bergese, Paolo
name: Bielska, Ewa
name: Blenkiron, Cherie
name: Bobis-Wozowicz, Sylwia
name: Boilard, Eric
name: Boireau, Wilfrid
name: Bongiovanni, Antonella
name: Borras, Francesc E
name: Bosch, Steffi
name: Boulanger, Chantal M
name: Breakefield, Xandra
name: Breglio, Andrew M
name: Brennan, Meadhbh A
name: Brigstock, David R
name: Brisson, Alain
name: Broekman, Marike LD
name: Bromberg, Jacqueline F
name: Bryl-Gorecka, Paulina
name: Buch, Shilpa
name: Buck, Amy H
name: Burger, Dylan
name: Busatto, Sara
name: Buschmann, Dominik
name: Bussolati, Benedetta
name: Buzas, Edit
name: Byrd, James Bryan
name: Camussi, Giovanni
name: Carter, David RF
name: Caruso, Sarah
name: Chamley, Lawrence W
name: Chang, Yu-Ting
name: Chen, Chihchen
name: Chen, Shuai
name: Sim, Lesley
name: Chin, Andrew R
name: Clayton, Aled
name: Clerici, Stefano P
name: Cocks, Alex
name: Cocucci, Emanuele
name: Coffey, Robert J
name: Cordeiro-da-Silva, Anabela
name: Couch, Yvonne
name: Coumans, Frank AW
name: Coyle, Beth
name: Crescitelli, Rossella
name: Criado, Miria Ferreira
name: D'Souza-Schorey, Crislyn
name: Das, Saumya
name: Chaudhuri, Amrita Datta
name: de Candia, Paola
name: De Santana Junior, Eliezer F
name: De Wever, Olivier
name: del Portillo, Hernando A
name: Demaret, Tanguy
name: Deville, Sarah
name: Devitt, Andrew
name: Dhondt, Bert
name: Di Vizio, Dolores
name: Dieterich, Lothar C
name: Dolo, Vincenza
name: Rubio, Ana Paula Dominguez
name: Dominici, Massimo
name: Dourado, Mauricio R
name: Driedonks, Tom AP
name: Duarte, Filipe
name: Duncan, Heather M
name: Eichenberger, Ramon M
name: Ekstrom, Karin
name: Andaloussi, Samir EL
name: Elie-Caille, Celine
name: Erdbrugger, Uta
name: Falcon-Perez, Juan M
name: Fatima, Farah
name: Fish, Jason E
name: Flores-Bellver, Miguel
name: Forsonits, Andras
name: Frelet-Barrand, Annie
name: Fricke, Fabia
name: Fuhrmann, Gregor
name: Gabrielsson, Susanne
name: Gamez-Valero, Ana
name: Gardiner, Chris
name: Gaertner, Kathrin
name: Gaudin, Raphael
name: Gho, Yong Song
name: Giebel, Bernd
name: Gilbert, Caroline
name: Gimona, Mario
name: Giusti, Ilaria
name: Goberdhan, Deborah C
name: Goergens, Andre
name: Gorski, Sharon M
name: Greening, David
name: Gross, Julia Christina
name: Gualerzi, Alice
name: Gupta, Gopal N
name: Gustafson, Dakota
name: Handberg, Aase
name: Haraszti, Reka A
name: Harrison, Paul
name: Hegyesi, Hargita
name: Hendrix, An
name: Hill, Andrew
name: Hochberg, Fred H
name: Hoffmann, Karl F
name: Holder, Beth
name: Holthofer, Harry
name: Hosseinkhani, Baharak
name: Hu, Guoku
name: Huang, Yiyao
name: Huber, Veronica
name: Hunt, Stuart
name: Ibrahim, Ahmed Gamal-Eldin
name: Ikezu, Tsuneya
name: Inal, Jameel M
name: Isin, Mustafa
name: Ivanova, Alena
name: Jackson, Hannah K
name: Jacobsen, Soren
name: Jay, Steven M
name: Jayachandran, Muthuvel
name: Jenster, Guido
name: Jiang, Lanzhou
name: Johnson, Suzanne M
name: Jones, Jennifer C
name: Jong, Ambrose
name: Jovanovic-Talisman, Tijana
name: Jung, Stephanie
name: Kalluri, Raghu
name: Kano, Shin-ichi
name: Kaur, Sukhbir
name: Kawamura, Yumi
name: Keller, Evan T
name: Khamari, Delaram
name: Khomyakova, Elena
name: Khvorova, Anastasia
name: Kierulf, Peter
name: Kim, Kwang Pyo
name: Kislinger, Thomas
name: Klingeborn, Mikael
name: Klinke, David J
name: Kornek, Miroslaw
name: Kosanovic, Maja M
name: Kovacs, Arpad Ferenc
name: Kraemer-Albers, Eva-Maria
name: Krasemann, Susanne
name: Krause, Mirja
name: Kurochkin, Igor
name: Kusuma, Gina D
name: Kuypers, Soren
name: Laitinen, Saara
name: Langevin, Scott M
name: Languino, Lucia R
name: Lannigan, Joanne
name: Lasser, Cecilia
name: Laurent, Louise C
name: Lavieu, Gregory
name: Lazaro-Ibanez, Elisa
name: Le Lay, Soazig
name: Lee, Myung-Shin
name: Lee, Yi Xin Fiona
name: Lemos, Debora S
name: Lenassi, Metka
name: Leszczynska, Aleksandra
name: Li, Isaac TS
name: Liao, Ke
name: Libregts, Sten F
name: Ligeti, Erzsebet
name: Lim, Rebecca
name: Lim, Sai Kiang
name: Line, Aija
name: Linnemannstoens, Karen
name: Llorente, Alicia
name: Lombard, Catherine A
name: Lorenowicz, Magdalena J
name: Lorincz, Akos M
name: Lotvall, Jan
name: Lovett, Jason
name: Lowry, Michelle C
name: Loyer, Xavier
name: Lu, Quan
name: Lukomska, Barbara
name: Lunavat, Taral R
name: Maas, Sybren LN
name: Malhi, Harmeet
name: Marcilla, Antonio
name: Mariani, Jacopo
name: Mariscal, Javier
name: Martens-Uzunova, Elena S
name: Martin-Jaular, Lorena
name: Martinez, M Carmen
name: Martins, Vilma Regina
name: Mathieu, Mathilde
name: Mathivanan, Suresh
name: Maugeri, Marco
name: McGinnis, Lynda K
name: McVey, Mark J
name: Meckes, David G
name: Meehan, Katie L
name: Mertens, Inge
name: Minciacchi, Valentina R
name: Moller, Andreas
name: Jorgensen, Malene Moller
name: Morales-Kastresana, Aizea
name: Morhayim, Jess
name: Mullier, Francois
name: Muraca, Maurizio
name: Musante, Luca
name: Mussack, Veronika
name: Muth, Dillon C
name: Myburgh, Kathryn H
name: Najrana, Tanbir
name: Nawaz, Muhammad
name: Nazarenko, Irina
name: Nejsum, Peter
name: Neri, Christian
name: Neri, Tommaso
name: Nieuwland, Rienk
name: Nimrichter, Leonardo
name: Nolan, John P
name: Hoen, Esther NM Nolte-'t
name: Hooten, Nicole Noren
name: O'Driscoll, Lorraine
name: O'Grady, Tina
name: O'Loghlen, Ana
name: Ochiya, Takahiro
name: Olivier, Martin
name: Ortiz, Alberto
name: Ortiz, Luis A
name: Osteikoetxea, Xabier
name: Ostegaard, Ole
name: Ostrowski, Matias
name: Park, Jaesung
name: Pegtel, D Michiel
name: Peinado, Hector
name: Perut, Francesca
name: Pfaffl, Michael W
name: Phinney, Donald G
name: Pieters, Bartijn CH
name: Pink, Ryan C
name: Pisetsky, David S
name: von Strandmann, Elke Pogge
name: Polakovicova, Iva
name: Poon, Ivan
name: Powell, Bonita H
name: Prada, Ilaria
name: Pulliam, Lynn
name: Quesenberry, Peter
name: Radeghieri, Annalisa
name: Raffai, Robert L
name: Raimondo, Stefania
name: Rak, Janusz
name: Ramirez, Marcel
name: Raposo, Graca
name: Rayyan, Morsi S
name: Regev-Rudzki, Neta
name: Ricklefs, Franz L
name: Robbins, Paul D
name: Roberts, David D
name: Rodrigues, Silvia C
name: Rohde, Eva
name: Rome, Sophie
name: Rouschop, Kasper MA
name: Rughetti, Aurelia
name: Russell, Ashley E
name: Saa, Paula
name: Sahoo, Susmita
name: Salas-Huenuleo, Edison
name: Sanchez, Catherine
name: Saugstad, Julie A
name: Saul, Meike J
name: Schiffelers, Raymond M
name: Schneider, Raphael
name: Schoyen, Tine Hiorth
name: Scott, Aaron
name: Shahaj, Eriomina
name: Sharma, Shivani
name: Shatnyeva, Olga
name: Shekari, Faezeh
name: Shelke, Ganesh Vilas
name: Shetty, Ashok K
name: Shiba, Kiyotaka
name: Siljander, Pia R-M
name: Silva, Andreia M
name: Skowronek, Agata
name: Snyder, Orman L
name: Soares, Rodrigo Pedro
name: Sodar, Barbara W
name: Soekmadji, Carolina
name: Sotillo, Javier
name: Stahl, Philip D
name: Stoorvogel, Willem
name: Stott, Shannon L
name: Strasser, Erwin F
name: Swift, Simon
name: Tahara, Hidetoshi
name: Tewari, Muneesh
name: Timms, Kate
name: Tiwari, Swasti
name: Tixeira, Rochelle
name: Tkach, Mercedes
name: Toh, Wei Seong
name: Tomasini, Richard
name: Torrecilhas, Ana Claudia
name: Tosar, Juan Pablo
name: Toxavidis, Vasilis
name: Urbanelli, Lorena
name: Vader, Pieter
name: van Balkom, Bas WM
name: van der Grein, Susanne G
name: Van Deun, Jan
name: van Herwijnen, Martijn JC
name: Van Keuren-Jensen, Kendall
name: van Niel, Guillaume
name: van Royen, Martin E
name: van Wijnen, Andre J
name: Vasconcelos, M Helena
name: Vechetti, Ivan J
name: Veit, Tiago D
name: Vella, Laura J
name: Velot, Emilie
name: Verweij, Frederik J
name: Vestad, Beate
name: Vinas, Jose L
name: Visnovitz, Tamas
name: Vukman, Krisztina V
name: Wahlgren, Jessica
name: Watson, Dionysios C
name: Wauben, Marca HM
name: Weaver, Alissa
name: Webber, Jason P
name: Weber, Viktoria
name: Wehman, Ann M
name: Weiss, Daniel J
name: Welsh, Joshua A
name: Wendt, Sebastian
name: Wheelock, Asa M
name: Wiener, Zoltan
name: Witte, Leonie
name: Wolfram, Joy
name: Xagorari, Angeliki
name: Xander, Patricia
name: Xu, Jing
name: Yan, Xiaomei
name: Yanez-Mo, Maria
name: Yin, Hang
name: Yuana, Yuana
name: Zappulli, Valentina
name: Zarubova, Jana
name: Zekas, Vytautas
name: Zhang, Jian-ye
name: Zhao, Zezhou
name: Zheng, Lei
name: Zheutlin, Alexander R
name: Zickler, Antje M
name: Zimmermann, Pascale
name: Zivkovic, Angela M
name: Zocco, Davide
name: Zuba-Surma, Ewa K
Publisher: name: La Trobe
Year: 2021

description: The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.