Additional file 1 of The treasure inside barley seeds: microbial diversity and plant beneficial bacteria
Creators: name: Bziuk, Nina
name: Maccario, Lorrie
name: Straube, Benjamin
name: Wehner, Gwendolin
name: Sørensen, Søren J.
name: Schikora, Adam
name: Smalla, Kornelia
Publisher: name: figshare
Year: 2021
Subjects: subject: Microbiology
subject: FOS: Biological sciences
subject: Genetics
subject: Plant Biology

description: Additional file 1. Table S1. Primer sequences. Table S2. Pielou’s evenness, Species richness and Shannon index of endophytic seed microbial communities obtained from seven different barley genotypes. Table S3. Dominant phyla in the endophytic seed microbiome of seven different barley genotypes. Table S4. Pielou’s evenness, Species richness and Shannon index of bulk soil and barley rhizosphere samples from seven different genotypes grown in mouldboard plough (MP) soil. Table S5. Pielou’s evenness, Species richness and Shannon index of bulk soil and barley rhizosphere samples from seven different genotypes grown in cultivator tillage (CT) soil. Table S6. PERMANOVA of each barley genotype of rhizosphere samples obtained from plants grown in mouldboard plough (MP) soil. Table S7. PERMANOVA of each versus each barley genotype of rhizosphere samples obtained from plants grown in cultivator tillage (CT) soil. Table S8. Dominant phyla in bulk soil and barley rhizosphere of seven different genotypes grown in two diverse soils (MP; CT). Table S9. Twenty most abundant genera in bulk soil and barley rhizosphere of seven different genotypes grown in two diverse soils (MP; CT). Figure S1. Rarefaction curves of 16S rRNA gene amplicon sequencing data of the barley seed microbiome. Figure S2. Microbial diversity of the endophytic seed microbiome varied between six barley genotypes originated from the same place. The microbial alpha-diversity indices Species richness (A) and Shannon diversity index (B) varied depending on the plant genotype (ANOVA for Species richness p ≤ 0.001 and Shannon index p ≤ 0.01). Beta-diversity of the endophytic seed microbiome was visualized by NMDS (C). The microbiome composition based on Bray-Curtis community dissimilarities (ASVs obtained from 16S rRNA gene amplicon sequencing) and was assessed from DNA of surface-sterilized barley seeds of six genotypes harvested at the same field site. Figure S3. Logarithm of colony forming units (CFUs)/g seed of seven different barley genotypes determined after 48 h incubation at 28 °C. 0.5 g of surface-sterilized seeds were ground and solved in 4.5 mL sterile double-distilled water. The seed suspension was plated on R2A supplemented with 100 µg/mL cycloheximide. Figure S4. BOX fingerprint of isolated endophytes taxonomically affiliated to the genus Curtobacterium. Figure S5. BOX fingerprint of isolated endophytes taxonomically affiliated to the genus Paenibacillus. Figure S6. Rarefaction curves of 16S rRNA gene amplicon sequencing data of barley rhizosphere and bulk soil samples from two different soils (MP: mouldboard plough; CT: cultivator tillage) and seven different genotypes. Figure S7. Shannon index of bulk soil and barley rhizosphere samples of seven different genotypes grown in MP and CT soils. Figure S8. Non-metric multidimensional scaling (NMDS) of barley rhizosphere microbial communities from seven different genotypes grown in MP (A) and CT (B) soil. The rhizosphere community composition based on Bray-Curtis dissimilarities and was obtained from ASVs. The seven different genotypes were grown in MP (mouldboard plough) and CT (cultivator tillage) soil until BBCH13. Respective bulk soil samples are not shown. ANOSIM verified significant differences between the genotypes (p ≤ 0.001)