cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Lessons learnt over two decades of vaccination against hepatitis B in Italy
Creators: name: Romanò, Luisa
name: Galli, Cristina
name: Tagliacarne, Catia
name: Zanetti, Alessandro Remo
Publisher: name: Department of Health Science - University of Genoa
Year: 2015
Subjects: Hepatitis B; Vaccination; HBV mutants

description: This article reviews topics covered and discussed at the Meeting: âVaccini e vaccinazioni. Migliorare lâoggi e preparare il domaniâ, held in Genoa, Italy, on 12 September 2014. Data presented at the meeting, clearly showed that: 1) hepatitis B vaccination can confer long-term protection and there is no need for booster in immunocompetent vaccinees; 2) vaccination is highly effective in protecting population from clinical acute or chronic HBV infections, including hepatocellular carcinoma; 3) children vaccinatedÂ as infants with hexavalent vaccines maintain immunological memory 5 years after priming, but further studies are needed to assess whether immunity persists during the adolescence and adulthood when risk of exposure to HBV becomes higher; 4) the emergence of vaccine-escape mutants and Pol-gene mutants during antiviral therapy - which can result in changes in the S-gene - is of some concern, but at present there is no evidence that such mutants may pose a threat to the established programs of vaccination.Â
description: Journal of Preventive Medicine and Hygiene, Vol 56, No 1 (2015)
Herpes Zoster: the rationale for the introduction of vaccination in Italy
Creators: name: Paganino, Chiara
name: Alicino, Cristiano
name: Trucchi, Cecilia
name: Albanese, Erika
name: Sticchi, Laura
name: Icardi, Giancarlo
Publisher: name: Department of Health Science - University of Genoa
Year: 2015
Subjects: Herpes Zoster; Vaccine; Italy

description: Herpes Zoster (HZ) and its main complication, post-herpetic neuralgia (PHN), represent an important public health issue because of their relevant burden within older adult population and the actual suboptimal therapeutic management of the diseases. Incidences of HZ and PHN are comparable all over the world and are closely related with the population age. Epidemiological data collected in Italy about HZ and its complications confirmed the trend registered in North America and Europe. Moreover HZ related burden is exacerbated by a significant economic impact related to both direct and indirect costs.

Since 2006 a live, attenuated varicella zoster virus vaccine, that contains VZV Oka strain [Zostavax, Merck & Co., Inc.], was licensed for the prevention of HZ and PHN in adults aged ≥ 60 years. Since 2011, the licensure has been extended to adults between 50 and 59 years. The vaccine has demonstrated a goodÂ immunogenicity, efficacy and safety profiles in two pivotal phase III clinical trials and the effectiveness was further confirmed after vaccine licensure. Pharmaco-economic studies concluded that HZ vaccine is cost-effective in most European countries and generally supported the economic value of this vaccination.

The vaccine is actually recommended in USA, Canada and several European countries. The opportunity to reduce the burden of these diseases by the recommendation of HZ vaccination have been evaluated and suggested also in our Country and some Regions have been recently introduced the vaccine in their immunization plan. If the good results, already obtained with HZ vaccine in other countries, will be confirmed by these Italian pilot experiences, vaccination programs should be made uniform in all Country in order to ensure an equitable offer of this important preventive tool.Â
description: Journal of Preventive Medicine and Hygiene, Vol 56, No 1 (2015)
Decennial diphtheria-tetanus adult boosters: are they really necessary?
Creators: name: Trucchi, Cecilia
name: Zoppi, Giorgio
Publisher: name: Department of Health Science - University of Genoa
Year: 2015
Subjects: Diphtheria-tetanus; Vaccine; Booster

description: Available epidemiological data shows that an average number of 59 cases of tetanus per year are still reported in Italy. Most of cases (80.2%) occur in subjects > 64 years-old. Furthermore, the proportion of females among subjects ≥ 65 years-old is significantly higher than males (87.7% vs. 64.4%, p < 0.0001). Seroprevalence data show that the percentage of subjects with protective tetanus antibody levels (> 0.1 IU/ml) decreases with increasing age. Most people aged ≥ 65 years are unprotected. The antibody levels are higher in males than females (p < 0.001) in subjects > 25 years-old.

Conversely, extensive childhood immunization and adequate boosting vaccination of adults led to the near-elimination of diphtheria in Western countries.

 The current Italian National Immunization Prevention Plan 2012-2014 recommends the administration of a primary vaccine course in the first year of life and booster at the preschool age, in adolescents and in adults every 10 years. Nevertheless, the need for decennial booster doses is debated by some experts, who state that the best time to offer a single dose of vaccine against tetanus and diphtheria is the age of 50, since low levels of antibody titers are observed.

Considering the availability of combined vaccines against diphtheria, tetanus and pertussis (DTaP or dTaP), and the increasing incidence of pertussis in infants, who are at highest risk of serious complications, in adolescents and in adults, some countries have introduced decennial dTaP in the adults immunization schedule. It is desirable that this recommendation is also introduced in the future Italian Immunization Prevention Plan.

The present review overviews the epidemiological data and the immunization strategies against tetanus, diphtheria and pertussis in Italy, discussing the rationale not only of decennial dT booster but also of the dTaP booster.Â
description: Journal of Preventive Medicine and Hygiene, Vol 56, No 1 (2015)
Is individual social deprivation is associated with adverse perinatal outcomes? Results of a French multicentre cross-sectional survey
Creators: name: Lelong, Audrey
name: Jiroff, Laetitia
name: Blanquet, Marie
name: Mourgues, Charline
name: Leymarie, Marie Christine
name: Gerbaud, Laurent
name: LÃ©Mery, Didier
name: Vendittelli, FranÃ§Oise
Publisher: name: Department of Health Science - University of Genoa
Year: 2015
Subjects: Adverse perinatal outcomes; Preterm birth; Individual Social deprivation; EPICES score

description: Introduction: French national health programmes take into account social deprivation in their implementation, those targeting perinatal outcomes, especially. The main aim of the present work was to assess the association between individual social deprivation and adverse perinatal outcomes. Â

Methods: A multicentre cross-sectional population-based survey was performed between October and December 2007. Eligible women delivered a baby in one of the three maternity hospitals of Clermont-Ferrand area, and read and spoke French fluently. Women who had undergone voluntary termination of pregnancy were excluded. Individual social deprivation was measured by the EPICES score. Standard prenatal follow-up defined by having less than 7 consultations and quality of prenatal care defined by having at least four consultations were measured. Adverse perinatal outcomes were measured by a composite criterion defined by women who had the occurrence of the three main causes of pregnancy-related disorders: preterm delivery, and/or diabetes, and/or obstetrical hypertension. Â

Results: Of the 471 eligible women, 464 were finally included. One hundred and fifteen (24.78%) women were socially deprived. The most deprived women had poor standard prenatal follow-up (p=0.003) and poor quality of prenatal care (0.03). Nationality was the sole confounding factor identified. Deprived women had a two-fold greater risk of adverse perinatal outcomes, adjusted odds ratio 1.95 [1.15; 3.29]. Â

Discussion: Social deprivation was associated with adverse perinatal outcomes. Social deprivation should be systematically screened in pregnant women standard follow-up, among migrant women, especially.
description: Journal of Preventive Medicine and Hygiene, Vol 56, No 2 (2015)
Limb-girdle muscular dystrophy type 2B causes HDL-C abnormalities in patients and statin-resistant muscle wasting in dysferlin-deficient mice
Creators: name: White, Zoe
name: Sun, Zeren
name: Sauge, Elodie
name: Cox, Dan
name: Donen, Graham
name: Pechkovsky, Dmitri
name: Straub, Volker
name: Francis, Gordon A.
name: Bernatchez, Pascal
Publisher: name: BioMed Central
Year: 2023

description: Limb-girdle muscular dystrophy (MD) type 2B (LGMD2B) and Duchenne MD (DMD) are caused by mutations to the Dysferlin and Dystrophin genes, respectively. We have recently demonstrated in typically mild dysferlin- and dystrophin-deficient mouse models that increased plasma cholesterol levels severely exacerbate muscle wasting, and that DMD patients display primary dyslipidemia characterized by elevated plasma cholesterol and triglycerides. Herein, we investigate lipoprotein abnormalities in LGMD2B and if statin therapy protects dysferlin-deficient mice (Dysf) from muscle damage. Herein, lipoproteins and liver enzymes from LGMD2B patients and dysferlin-null (Dysf) mice were analyzed. Simvastatin, which exhibits anti-muscle wasting effects in mouse models of DMD and corrects aberrant expression of key markers of lipid metabolism and endogenous cholesterol synthesis, was tested in Dysf mice. Muscle damage and fibrosis were assessed by immunohistochemistry and cholesterol signalling pathways via Western blot. LGMD2B patients show reduced serum high-density lipoprotein cholesterol (HDL-C) levels compared to healthy controls and exhibit a greater prevalence of abnormal total cholesterol (CHOL)/HDL-C ratios despite an absence of liver dysfunction. While Dysf mice presented with reduced CHOL and associated HDL-C and LDL-C-associated fractions, simvastatin treatment did not prevent muscle wasting in quadriceps and triceps muscle groups or correct aberrant low-density lipoprotein receptor (LDLR) and 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) protein expression. LGMD2B patients present with reduced serum concentrations of HDL-C, a major metabolic comorbidity, and as a result, statin therapy is unlikely to prevent muscle wasting in this population. We propose that like DMD, LGMD2B should be considered as a new type of genetic dyslipidemia.
Occupational Medicine and Hygiene: Applied Research in Italy
Creators: name: Copello, Francesco
name: Garbarino, Sergio
name: Messineo, Agostino
name: Campagna, Marcello
name: Durando, Paolo
Publisher: name: Department of Health Science - University of Genoa
Year: 2015
Subjects: stress and violence; biological risk; sleep hygiene

description: The goal of Occupational Medicine and Hygiene is that of ensuring safety, health and well-being at workplaces, mainly assessing and preventing existing occupational risk. Scientific research in this field can provide useful arguments and further evidence upon which effective, efficient and sustainable policies and prevention measures have to be chosen and applied by the occupational physician in work-life. This paper summarizes four original studies, conducted in different professional settings across Italy, focusing critical items, such as stress and violence, biological risk and sleep hygiene. The knowledge obtained can be useful to orientate proper prevention programs aimed at improving workplace health.
description: Journal of Preventive Medicine and Hygiene, Vol 56, No 2 (2015)
A comparative study on interactions of ionic collectors with orthoclase
Creators: name: Ozun, Savas
name: Atalay, Mustafa Umit
Publisher: name: Faculty of Geoengineering, Mining and Geology, Wrocław University of Technology, Wrocław
Year: 2016
Subjects: collector interactions
zeta potential
microflotation, FTIR
AFM
orthoclase

description: Physicochemical Problems of Mineral Processing; ISSN 2084-4735
title: Supplementary Evidence: Climate Change Plan Update
Creators: name: Haszeldine, R Stuart
Publisher: name: SCCS
Year: 2021
Subjects: subject: CCS
subject: climate
subject: climate change
subject: scottish parliament
subject: evidence
subject: energy
subject: economy
subject: committee

description: Supplementary evidence submitted to the Scottish Parliament Economy, Energy and Fair Work Committee by Stuart Haszeldine, Director of SCCS, and Professor of Carbon Capture and Storage at the School of GeoSciences, University of Edinburgh.
description: Supplementary evidence submitted to the Scottish Parliament Economy, Energy and Fair Work Committee by Stuart Haszeldine, Director of SCCS, and Professor of Carbon Capture and Storage at the School of GeoSciences, University of Edinburgh.
Finalised version of the conceptual framework
Creators: name: Wehn, Uta
name: Gharesifard, Mohammad
name: Somerwill, Luke
Publisher: name: Zenodo
Year: 2021
Subjects: Impact assessment
method
citizen science
evaluation
indicator

description: This report presents the draft MICS conceptual framework which constitutes the overarching structure within which novel and appropriate impact assessment methods will be provided for citizen science projects, including the MICS case studies, and which will inform the MICS online platform.
description: This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement No 824711.
Mineral matter in municipal solid waste
Creators: name: Sniadecka, Nikola
name: Tonderski, Andrzej
name: Hanel, Andreas
name: Wojda-Gburek, Jacek
name: Hupka, Jan
Publisher: name: Faculty of Geoengineering, Mining and Geology, Wrocław University of Technology, Wrocław
Year: 2016
Subjects: mineral matter
municipal waste
separation technologies
size distribution

description: Physicochemical Problems of Mineral Processing; ISSN 2084-4735
Purification of halloysite by magnetic separation
Creators: name: Sakiewicz, Piotr
name: Lutynski, Marcin
name: Soltys, Jozef
name: Pytlinski, Andrzej
Publisher: name: Faculty of Geoengineering, Mining and Geology, Wrocław University of Technology, Wrocław
Year: 2016
Subjects: halloysite
magnetic separation
HCl treatment

description: Physicochemical Problems of Mineral Processing; ISSN 2084-4735
title: Community Learning Groups: a Method in Adult Literacy for Community Participation and Learning
Creators: name: Hamutenya, Sirkka Kapango
Publisher: name: University of Massachusetts Amherst
Year: 1996
Subjects: subject: Community
subject: Learning
subject: Groups
subject: Method
subject: Adult Literacy
subject: Participation
subject: Adult and Continuing Education and Teaching
subject: Bilingual, Multilingual, and Multicultural Education
subject: Education
subject: Educational Methods
subject: Educational Psychology

description: The study was done in the context of the Namibia National Literacy Program and was influenced by my working environment. While studying adult education, and by being exposed to a variety of philosophical views and ideas, I tried to reflect those views and ideas to the adult learner groups who graduated from the Namibia National Literacy Program as well as to those in the communities not attending any classes. The questions I had in my mind were the following: What can they do together to learn? How can they utilize their new skills to get involved in participatory community development. The study explores the community groups and how to utilize them as a method of adult literacy education for educational purposes and community participation. This document addressed the following; 1) Background information and purpose of study; 2) literature review section of previous work done on the topic and how it relates to the current study; 3) the research procedure and the methodology used in the field to gather the data; 4) case studies on the formal and informal group learning involved in the study; 5) understandings and implications of findings on Community Learning Groups; and 6) recommendations based on those findings.
IMPROVEMENT AND DEVELOPMENT OF INNOVATIVE PURIFICATION TECHNIQUES OF BIOPOLYMERS RECOVERED FROM FERMENTATIVE BROTH
Creators: name: Lauder, Anja
Publisher: name: Zenodo
Year: 2022
Subjects: Polyhydroxyalknoates extraction

description: Polyhydroxyalkanoates (PHAs) are biodegradable polymers produced by various microorganisms. The separation of PHAs is one of the most important key factors in the design of a sustainable PHA production. This thesis evaluated different separation methods for the recovery of polyhydroxybutyrate (PHB) from three different fermentative broths. A DSP process including SDS treatment, homogenization, chemical digestion with NaOH or H2SO4 and methanol or ethanol polishing was selected as the best combination for the PHB recovery. PHB of 99,8 % purity was recovered from FB1 broth, with a recovery yield of 71,11 %, after 0,025 g SDS/ g DM, homogenization (800 bar, 2 passes), digestion (0,05 M NaOH, 1 h, RT) and methanol washing. PHB of 94,2 % purity was recovered from FB2 broth with a yield of 53,27 %, after 0.05 g SDS/ g DM, homogenization (800 bar, 2 passes), digestion (0,7 M H2SO4, 6 h, 90°C) and methanol washing. FB3 yielded PHB with a purity of 99,2 % and recovery of 78,3 %, using the same processing conditions as FB2, except it was washed with ethanol.
Holographic BCFT spectra from brane mergers
Creators: name: Biswas, Shovon
name: Kastikainen, Jani
name: Shashi, Sanjit
name: Sully, James
Publisher: name: Springer Berlin Heidelberg
Year: 2023

description: We use holography to study the spectra of boundary conformal field theories (BCFTs). To do so, we consider a 2-dimensional Euclidean BCFT with two circular boundaries that correspond to dynamical end-of-the-world branes in 3-dimensional gravity. Interactions between these branes inform the operator content and the energy spectrum of the dual BCFT. As a proof of concept, we first consider two highly separated branes whose only interaction is taken to be mediated by a scalar field. The holographic computation of the scalar-mediated exchange reproduces a light scalar primary and its global descendants in the closed-string channel of the dual BCFT. We then consider a gravity model with point particles. Here, the interaction of two separated branes corresponds to a heavy operator which lies below the black hole threshold. However, we may also consider branes at finite separation that “merge” non-smoothly. Such brane mergers can be used to describe unitary sub-threshold boundary-condition-changing operators in the open-string spectrum of the BCFT. We also find a new class of sub-threshold Euclidean bra-ket wormhole saddles with a factorization puzzle for closed-string amplitudes.
Epidemiology of cancers of infectious origin and prevention strategies
Creators: name: De Flora, Silvio
name: La Maestra, Sebastiano
Publisher: name: Department of Health Science - University of Genoa
Year: 2015
Subjects: Cancer; Infectious diseases; Prevention

description: Infectious and parasitic diseases represent the third cause of cancer worldwide. A number of infectious and parasitic agents have been suspected or recognized to be associated with human cancers, including DNA viruses, such as papillomaviruses (several HPV types), herpesviruses (EBV and KSHV), polyomaviruses (SV40, MCV, BK, and JCV), and hepadnaviruses (HBV); RNA viruses, such as flaviviruses (HCV), defective viruses (HDV), and retroviruses (HTLV-I, HTLV-II, HIV-1, HIV-2,HERV-K, and XMRV); bacteria, such as H. pylori, S. typhi, S. bovis, Bartonella, and C. pneumoniae; protozoa, such as P. falciparum; trematodes, such as S. haematobium, S. japonicum, S. mansoni, O. viverrini, O. felineus, and C. sinensis. Each one of the chronic infections with H. pylori, HPV, and HBV/HCV is responsible for approxi- mately the 5% of all human cancers. The primary preventionÂ of infection-related cancers is addressed both to avoidance and eradication of chronic infections and to protection of the host organism. Vaccines provide fundamental tools for the prevention of infectious diseases and related cancers. The large-scale application of the HBV vaccine has already shown to favorably affect the epidemiological burden of primary hepatocellular carcinoma, and HPV vaccines have specifically been designed in order to prevent cervical cancer and other HPV-related cancers. The secondary prevention of infection-associated cancers has already found broad applications in the control of cervical cancer. Detection of early gastric cancer by endoscopy has been applied in Asian countries. Avoidance of local relapses, invasion, and metastasis may be achieved by applying tertiary prevention, which targets specific mechanisms, such as angiogenesis.Â
description: Journal of Preventive Medicine and Hygiene, Vol 56, No 1 (2015)
PhD thesis: Tecnologías de vanguardia para la producción de bioplásticos a partir de residuos complejos
Creators: name: Herreros, Natalia Hernandez
Publisher: name: Zenodo
Year: 2022
Subjects: polyhydroxyalkanoates; Cupriavidus necator; volatile fatty acids

description: This Doctoral Thesis has been carried out in the context of the European project "AFTERLIFE" (Advanced Filtration Technologies for the Recovery and Later conversion of relevant Fractions from wastEwater) where the development of a flexible and efficient process is proposed for the recovery and revalorization of different fractions from wastewater of agro-industrial origin. In this context, and the first chapter of this report, the bacterium Cupriavidus necator H16 was selected for the transformation of volatile fatty acids (VFAs), derived from the anaerobic digestion (AD) of wastewater, into bioplastics from microbial origin or polyhydroxyalkanoates (PHA). Through the characterization of the raw materials and the study of the tolerance limits to the major components of the waste, the production of PHA was maximized in a bioreactor at a scale of 2 L. Applying a strategy of feeding coupled to biomass production and specific uptake rate of the carbon sources available in the wastewater, a PHA accumulation of 80 % (w/w) was achieved with respect to the biomass dry weight, as well as a 0.8 g-1L-1h-1 yield.
Cancer prevention: state of the art and future prospects
Creators: name: Valle, Ivana
name: Tramalloni, Daniela
name: Bragazzi, Nicola
Publisher: name: Department of Health Science - University of Genoa
Year: 2015
Subjects: Cancer prevention;Organized screening program; Vaccine

description: Cancer imposes a heavy societal burden worldwide, in terms of both epidemiology and costs. The introduction of more sophisti- cated imaging and diagnostic techniques and advanced drugs that specifically target tumor cells is leading to increasingly expensive treatments, which may be affordable only for few patients. Pre- vention, and particularly primary prevention, is an effective way of addressing the challenging issue of cancer, since between a third and a half of cancers could be prevented on the basis of our current knowledge of risk factors. Moreover, prevention is cost-effective, its effects are not limited to high-risk subjects but extend to the entire population, and it is not dependent on socio- economic status. Regulatory measures can have a broad impact, even on future generations; by empowering and educating sub- jects, promoting healthy behaviours and teaching self-care, they can trigger a virtuous cycle. In recent decades, oncology has shifted from being merely reactive to being proactive; this shift has led to the development of so-called âP4 medicineâ, where the 4 Ps stand for âpreventiveâ, âpredictiveâ, âpersonalizedâ and âparticipatoryâ. Prevention programs are an important part of the effort to control cancer, as they are able to reduce both the incidence of cancer and mortality. For instance, screening for colorectal, breast and cervical cancer is reducing the burden of these common tumors. Anti-cancer vaccines, both prophylactic and therapeutic, constitute another important preventive tool. Although progress has been made in these areas, much remains to be done. With regard to screening programs, coverage could be increased by introducing new, more acceptable, less invasive tests, stratifying screening through correlation with anamnestic, clinical, radiological and genomic data (so-called âpopulation- based personalized cancer screeningâ), and exploiting new infor- mation and communication technologies, such as smartphone applications or personalized text messages (so-called âscreening 2.0â). Advocacy and recommendations by physicians can also play a role, in that eligible subjects need to be able to discuss their doubts and their perceived psycho-social barriers. How- ever, new screening initiatives should be implemented only after a careful health technology assessment has been performed within the framework of evidence-based medicine, organized screening programs have been strengthened and opportunistic or spontane- ous programs have been limited.
description: Journal of Preventive Medicine and Hygiene, Vol 56, No 1 (2015)
Insights on common vaccinations in HIV-infection: efficacy and safety
Creators: name: Nicolini, Laura Ambra
name: Giacobbe, Daniele Roberto
name: Di Biagio, Antonio
name: Viscoli, Claudio
Publisher: name: Department of Health Science - University of Genoa
Year: 2015
Subjects: Vaccination; HIV; Efficacy

description: HIV-infected patients are at increased risk for both vaccine-preventable diseases and their complications, with mortality rates higher than in non-HIV-infected individuals. Consequently, international guidelines generally recommend inactivated vaccines in HIV-patients, even if HIV-related immunodeficiency may impair efficacy; live vaccines are usually not recommended in these patients because of safety concerns. The aim of this short article is to review current knowledge about both efficacy and safety of vaccines in HIV-infected individuals.Â
description: Journal of Preventive Medicine and Hygiene, Vol 56, No 1 (2015)
Influenza vaccination in the elderly: why are the overall benefits still hotly debated?
Creators: name: Trucchi, Cecilia
name: Paganino, Chiara
name: Orsi, Andrea
name: De Florentis, Daniela
name: Ansaldi, Filippo
Publisher: name: Department of Health Science - University of Genoa
Year: 2015
Subjects: Influenza vaccine; Vaccine efficacy; Vaccine effectiveness

description: The exact magnitude of the benefit of influenza vaccine among elderly individuals is subject of considerable debated. Existing vaccine effectiveness estimates come mostly from observational studies, which may be biased because of difficulties in identifying and adjusting for confounders. In this paper, we examine the potential sources of bias in observational studies of influenza vaccine effectiveness in the elderly and we discuss availableÂ evidence regarding the efficacy and effectiveness of licensed influenza vaccines. Although several methodological criticisms among the available analyses on seasonal vaccines for elderly were identified, overall seasonal influenza vaccines showed relevant efficacy/effectiveness in reducing the risk of influenza and its complications in the elderly, considering different measure of outcome.Â
description: Journal of Preventive Medicine and Hygiene, Vol 56, No 1 (2015)
Optimizing DNA extraction protocols for the diet analysis of a baleen whale (Eubalaena australis)
Creators: name: Parikh, Aashi
name: O'Rorke, Richard
name: Carroll, Emma
name: Vermeulen, Els
name: Harcourt, Robert
name: Chariton, Anthony
Publisher: name: Zenodo
Year: 2024
Subjects: Faecal metabarcoding
southern right whale
phosphate buffer extraction
PowerSoil
QIAamp Fast DNA Stool Mini Kit

description: Faecal metabarcoding is widely used for mammalian diet analysis. However, most extraction protocols are designed to use high molecular weight genomic DNA, making short sequences of digested DNA challenging to extract. Here, we compared a phosphate buffer DNA extraction method along with two commercial extraction kits (the QIAamp Fast DNA Stool Mini Kit and the PowerSoil kit) with the following variations: 1) different durations of incubation in a phosphate buffer (1 hour and 24 hours), 2) processing of both pellet and supernatant from phosphate buffer incubation, and 3) two different concentrations of DNA binding buffer to examine prey DNA in southern right whale (Eubalaena australis, SRW) faecal matter. We found that the choice of extraction protocol influenced richness, diversity, and composition of eukaryotes (18S rDNA) and crustaceans (Crust16S mtDNA) detected in SRW faecal samples. The PowerSoil protocol performed well for both markers, delivering the highest target richness for 18S rDNA and highest diversity for Crust16S mtDNA, while the pellet of the phosphate buffer yielded the highest richness for Crust16S mtDNA. The phosphate buffer supernatant protocols produced the lowest results for richness and diversity. Taxonomic composition in the supernatant alone was affected by the duration of incubation and the concentration of binding buffer and was also distinct from the corresponding pellet. Our results reinforce the importance of having clear targets prior to selecting a method for faecal metabarcoding, as the specific aims (e.g. identifying richness versus diversity) will inform the choice of extraction protocol.
description: Southern right whale faecal samples were collected opportunistically over decades of research. Faecal samples underwent DNA extraction using three different methods (phosphate buffer extraction, PowerSoil kit and QIAamp FAST DNA Stool Mini Kit) with modifications in each leading to 12 unique protocols. The modifications included: incubating samples for 1 hr and 24 hrs in a phosphate buffer, processing both the pellet and supernatant from phosphate buffer incubation, and the addition of 1x and 2x DNA binding buffer to the silica column. Extracted samples were amplified via a universal 18S and crustacean-specific 16S marker and sequenced. Analyses for taxonomic richness, diversity, and composition were performed in R to compare results from the different protocols.
Supplementary document for Mono-cycle terahertz pulses from intersubband shift currents in asymmetric semiconductor quantum wells - 5561584.pdf
Creators: name: Runge, Matthias
name: Kang, Tahee
name: Biermann, Klaus
name: Reimann, Klaus
name: Woerner, Michael
name: Elsaesser, Thomas
Publisher: name: Optica Publishing Group
Year: 2025
Subjects: Uncategorized

description: supplemental document 1
Optimizing DNA extraction protocols for the diet analysis of a baleen whale (Eubalaena australis)
Creators: name: Parikh, Aashi
name: O'Rorke, Richard
name: Carroll, Emma
name: Vermeulen, Els
name: Harcourt, Robert
name: Chariton, Anthony
Publisher: name: Zenodo
Year: 2025
Subjects: Faecal metabarcoding
southern right whale
phosphate buffer extraction
PowerSoil
QIAamp Fast DNA Stool Mini Kit

description: Faecal metabarcoding is widely used for mammalian diet analysis. However, most extraction protocols are designed to use high molecular weight genomic DNA, making short sequences of digested DNA challenging to extract. Here, we compared a phosphate buffer DNA extraction method along with two commercial extraction kits (the QIAamp Fast DNA Stool Mini Kit and the PowerSoil kit) with the following variations: 1) different durations of incubation in a phosphate buffer (1 hour and 24 hours), 2) processing of both pellet and supernatant from phosphate buffer incubation, and 3) two different concentrations of DNA binding buffer to examine prey DNA in southern right whale (Eubalaena australis, SRW) faecal matter. We found that the choice of extraction protocol influenced richness, diversity, and composition of eukaryotes (18S rDNA) and crustaceans (Crust16S mtDNA) detected in SRW faecal samples. The PowerSoil protocol performed well for both markers, delivering the highest target richness for 18S rDNA and highest diversity for Crust16S mtDNA, while the pellet of the phosphate buffer yielded the highest richness for Crust16S mtDNA. The phosphate buffer supernatant protocols produced the lowest results for richness and diversity. Taxonomic composition in the supernatant alone was affected by the duration of incubation and the concentration of binding buffer and was also distinct from the corresponding pellet. Our results reinforce the importance of having clear targets prior to selecting a method for faecal metabarcoding, as the specific aims (e.g. identifying richness versus diversity) will inform the choice of extraction protocol.
description: Southern right whale faecal samples were collected opportunistically over decades of research. Faecal samples underwent DNA extraction using three different methods (phosphate buffer extraction, PowerSoil kit and QIAamp FAST DNA Stool Mini Kit) with modifications in each leading to 12 unique protocols. The modifications included: incubating samples for 1 hr and 24 hrs in a phosphate buffer, processing both the pellet and supernatant from phosphate buffer incubation, and the addition of 1x and 2x DNA binding buffer to the silica column. Extracted samples were amplified via a universal 18S and crustacean-specific 16S marker and sequenced. Analyses for taxonomic richness, diversity, and composition were performed in R to compare results from the different protocols.
Supplementary document for Mono-cycle terahertz pulses from intersubband shift currents in asymmetric semiconductor quantum wells - 5561584.pdf
Creators: name: Runge, Matthias
name: Kang, Tahee
name: Biermann, Klaus
name: Reimann, Klaus
name: Woerner, Michael
name: Elsaesser, Thomas
Publisher: name: Optica Publishing Group
Year: 2021
Subjects: Uncategorized

description: supplemental document 1
: Una relectura del método sociológico: Emile Durkheim y el estudio científico de las formaciones sociales
Creators: name: Gavilanes Gavilanez, Andrea Alejandra
Publisher: name: unknown
Year: 2024

description: El artículo de Lorente Fernández ofrece una clara y concisa presentación de las ideas principales de Durkheim sobre el método sociológico y su impacto en la antropología. El autor logra sintetizar los conceptos clave de "Las reglas del método sociológico", proporcionando ejemplos y explicaciones que facilitan la comprensión del texto original.
History and Future Directions of the “Sylvia” Think Tank
Creators: name: Anderson, Joan
name: McDonald, Heather
name: Rodney, Patricia, 1955-
name: Dhari, Ranjit
name: Sawatzky-Girling, Brenda
Publisher: name: The University of British Columbia
Year: 2022

description: The “Sylvia” Think Tank (STT) is comprised of a self-funded, diverse, intergenerational and interdisciplinary group of academics, health care and social service practitioners, and community leaders involved in social justice-related advocacy work. Our first meeting was held at the Sylvia Hotel in Vancouver in 2015, and we have continued to meet there in subsequent years — except during the pandemic, when we met virtually — hence the name, the “Sylvia” Think Tank. The founders included Joan Anderson, Annette Browne, Heather McDonald, Patricia (Paddy) Rodney, and Sheryl Reimer-Kirkham. All shared a commitment to promoting equity and social justice in health care and human services. The STT has since expanded to include other academics, clinicians, health care users, community leaders, NGOs and students. Heather McDonald administered the STT from its inception to 2021. In 2022 Ranjit Kaur Dhari and Brenda Sawatzky-Girling graciously agreed to share the leadership of the STT, with Brenda Sawatzky-Girling managing the finances. The document below provides a description and analyses of our activities.
Feynman-Vernon influence functional approach to quantum transport in interacting nanojunctions: An analytical hierarchical study
Creators: name: Magazzù, Luca
name: Grifoni, Milena
Publisher: name: Universität Regensburg
Year: 2022
Subjects: subject: Kondo effect, Quantum transport, Quantum dots, Path-integral methods
subject: 530 Physik

description: We present a nonperturbative and formally exact approach for the charge transport in interacting nanojunctions based on a real-time path-integral formulation of the reduced system dynamics. For reservoirs of noninteracting fermions, the exact trace over the leads' degrees of freedom results in the time-nonlocal Feynman-Vernon influence functional, a functional of the Grassmann-valued paths of the nanojunction, which induces correlations among the tunneling transitions in and out of the nanojunction. An expansion of the influence functional in terms of the number of tunneling transitions, and integration of the Grassmann variables between the tunneling times, allows us to obtain a still exact generalized master equation for the populations of the reduced density matrix in the occupation-number representation, as well as a formally exact expression for the current. By borrowing the nomenclature of the famous spin-boson model, we parametrize the two-state dynamics of each single-particle fermionic degree of freedom, in the occupation-number representation, in terms of blips and sojourns. We apply our formalism to the exactly solvable resonant level model (RLM) and to the single-impurity Anderson model (SIAM), the latter being a prototype system for studying strong correlations. For both systems, we demonstrate a hierarchical diagrammatic structure. While the hierarchy closes at the second tier for the RLM, this is not the case for the interacting SIAM. Upon inspection of the current kernel, known results from various perturbative and nonperturbative approximation schemes to quantum transport in the SIAM are recovered. Finally, a noncrossing approximation for the hierarchical kernel is developed, which enables us to systematically decrease temperature at each next level of the approximation. Analytical results for a simplified fourth-tier scheme are presented both in equilibrium and nonequilibrium and with an applied magnetic field.
Age-dependent formation of TMEM106B amyloid filaments in human brains.
Creators: name: Schweighauser, Manuel
name: Arseni, Diana
name: Bacioglu, Mehtap
name: Huang, Melissa
name: Lövestam, Sofia
name: Shi, Yang
name: Yang, Yang
name: Zhang, Wenjuan
name: Kotecha, Abhay
name: Garringer, Holly J
name: Vidal, Ruben
name: Hallinan, Grace I
name: Newell, Kathy L
name: Tarutani, Airi
name: Murayama, Shigeo
name: Miyazaki, Masayuki
name: Saito, Yuko
name: Yoshida, Mari
name: Hasegawa, Kazuko
name: Lashley, Tammaryn
name: Revesz, Tamas
name: Kovacs, Gabor G
name: Van Swieten, John
name: Takao, Masaki
name: Hasegawa, Masato
name: Ghetti, Bernardino
name: Spillantini, Maria Grazia
name: Ryskeldi-Falcon, Benjamin
name: Murzin, Alexey G
name: Goedert, Michel
name: Scheres, Sjors HW
Publisher: name: Apollo - University of Cambridge Repository
Year: 2022
Subjects: Article
/631/535/1258/1259
/631/378/340
/101/28
/13/51
article

description: Many age-dependent neurodegenerative diseases, such as Alzheimer's and Parkinson's, are characterized by abundant inclusions of amyloid filaments. Filamentous inclusions of the proteins tau, amyloid-β, α-synuclein and transactive response DNA-binding protein (TARDBP; also known as TDP-43) are the most common1,2. Here we used structure determination by cryogenic electron microscopy to show that residues 120-254 of the lysosomal type II transmembrane protein 106B (TMEM106B) also form amyloid filaments in human brains. We determined the structures of TMEM106B filaments from a number of brain regions of 22 individuals with abundant amyloid deposits, including those resulting from sporadic and inherited tauopathies, amyloid-β amyloidoses, synucleinopathies and TDP-43 proteinopathies, as well as from the frontal cortex of 3 individuals with normal neurology and no or only a few amyloid deposits. We observed three TMEM106B folds, with no clear relationships between folds and diseases. TMEM106B filaments correlated with the presence of a 29-kDa sarkosyl-insoluble fragment and globular cytoplasmic inclusions, as detected by an antibody specific to the carboxy-terminal region of TMEM106B. The identification of TMEM106B filaments in the brains of older, but not younger, individuals with normal neurology indicates that they form in an age-dependent manner.
Data Management Plan for PhD "Social Infrastructuring Camden: Experiments in place making, community wealth building and relational learning"
Creators: name: Holland-Gilbert, Jasmine
Publisher: name: The Open University
Year: 2025
Subjects: classificationCode: 330405
subject: Public participation and community engagement

description: Within my PhD I am exploring Camden Council's We Make Camden Strategy, with a focus on community organising and social action. In particular, I will be analysing the work of the Participation and Partnerships Team, in relation to place making, community wealth building and relational learning. The Participation and Partnership's Team's directive is to develop and support participation and partnerships with and between Camden Council departments, Camden residents, Camden Industry and Camden voluntary and community organisations. A central focus within this directive is to support the development of, and engagement with, social infrastructure across the borough.This Data Managment Plan is for a series of scoping and co-design workshops which I will be facilitating with Camden Council's Participation and Partnersh Team. The purpose of these workshops will be to co-design the focus and direction of the wider research project, thereby ensuring that it is directly relevant and beneficial to the Participation and Partnerships Team’s work and Camden Council’s wider focus on achieving the We Make Camden Missions.
How well do you know your dopamine antagonists?
Creators: name: Therapeutics Initiative (University Of British Columbia)
Publisher: name: The University of British Columbia
Year: 2023

description: Background: Therapeutics Letter 139 aims to provide clinicians with an understanding of dopamine (DA) antagonists and their potential side effects. Dopamine antagonists are a class of drugs commonly used to treat various psychiatric disorders, such as schizophrenia and bipolar disorder. Methods: The authors reviewed the literature on dopamine antagonists and their side effects, summarized the mechanism of action of dopamine antagonists, their clinical uses, and the different classes of dopamine antagonists. A clinical pharmacological approach was used to review how people may be harmed by DA antagonists. Videos included with this Letter may help clinicians and students better understand the potential harms of this drug class. Results: The article highlights the different types of DA antagonists and their respective side effects. It notes that some dopamine antagonists can cause metabolic disturbances, such as weight gain and hyperglycemia. Movement disorders, such as Parkinsonism and tardive dyskinesia, are also common side effects. Moreover, some dopamine antagonists can have cardiac effects, such as QT prolongation and torsades de pointes. Conclusions: Clinicians should be aware of the potential harms associated with these drugs, especially metabolic, movement, and cardiac effects and should learn and teach the symptoms and signs of dopamine blockade. The potential benefits and risks of dopamine antagonists should be carefully weighed when considering their use and patients should be re-examined frequently during treatment, with dose and duration of treatment re-assessed when renewing prescriptions.
Supplementary document for Shuffled Rolling Shutter for Snapshot Temporal Imaging - 5561870.pdf
Creators: name: Vera, Esteban
name: Guzmán, Felipe
name: Diaz, Nelson
Publisher: name: Optica Publishing Group
Year: 2025
Subjects: Photonics and Electro-Optical Engineering (excl. Communications)
Photonics, Optoelectronics and Optical Communications
Image Processing

description: Supplementary Material
Nooralia San Martin 2002
Creators: name: Guillermo San Martin
Publisher: name: Zenodo
Year: 2005
Subjects: Biodiversity
Taxonomy
Animalia
Annelida
Polychaeta
Phyllodocida
Syllidae
Nooralia

description: Genus Nooralia San Martín, 2002
Nooralia San Martín, 2002: 333.
Type species. Nooralia bulgannabooyanga San Martín, 2002.
Diagnosis. Body small, short, with about 30 chaetigers. Surface of body smooth. Prostomium with 4 eyes and 3 antennae. Palps fused at bases. Two pairs of tentacular cirri. Antennae, tentacular cirri and dorsal cirri of chaetiger 1 long, cylindrical to spindle-shaped; remaining dorsal cirri short, lanceolate. Parapodia with dorsal simple capillary chaetae and compound chaetae with unidentate and bidentate short blades. Ventral simple chaetae apparently absent. Pharynx long, unarmed, with a crown of soft papillae on anterior rim. Proventricle small, difficult to see. Pygidium with 2 large anal cirri. Females brooding eggs dorsally, by means of compound notochaetae.
Remarks. The relationship of Nooralia to other members of the group is difficult to elucidate, because the genus displays features that differentiate it from all other Exogoninae genera, such as an indistinct proventricle and absence of a pharyngeal tooth. Characters such as the shape of the dorsal cirri, smooth dorsal surface and dorsal brooding of egg, as well as the shape of the aciculae of the single known species, suggest that it may be related to the genus Salvatoria. However, Salvatoria has palps fused by means of a dorsal membrane and a distinctly massive proventricle, which is long and large, and has a pharyngeal tooth. Furthermore, the compound chaetae of Nooralia are different to those of all other species of the genera included in the Exogoninae. The genus appears to have an isolated position in the Syllidae.
description: Published as part of Guillermo San Martin, 2005, Exogoninae (Polychaeta: Syllidae) from Australia With the Description of a New Genus and Twenty-two New Species, pp. 39-152 in Records of the Australian Museum 57 on page 45, DOI: 10.3853/j.0067-1975.57.2005.1438, http://zenodo.org/record/15343387
HyClear : A Novel Tissue Clearing Solution for One-Step Clearing of Microtissues
Creators: name: Nasseri, S. Soroush
name: Siren, Erika M. J.
name: Kizhakkedathu, Jayachandran N.
name: Cheung, Karen C.
Publisher: name: Multidisciplinary Digital Publishing Institute
Year: 2023

description: 3-D cell cultures are being increasingly used as in vitro models are capable of better mimicry of in vivo tissues, particularly in drug screenings where mass transfer limitations can affect the cancer biology and response to drugs. Three-dimensional microscopy techniques, such as confocal and multiphoton microscopy, have been used to elucidate data from 3-D cell cultures and whole organs, but their reach inside the 3-D tissues is restrained by the light scattering of the tissues, limiting their effective reach to 100–200 µm, which is simply not enough. Tissue clearing protocols, developed mostly for larger specimens usually involve multiple steps of viscous liquid submersion, and are not easily adaptable for much smaller spheroids and organoids. In this work, we have developed a novel tissue clearing solution tailored for small spheroids and organoids. Our tissue clearing protocol, called HyClear, uses a mixture of DMSO, HPG and urea to allow for one-step tissue clearing of spheroids and organoids, and is compatible with high-throughput screening studies due to its speed and simplicity. We have shown that our tissue clearing agent is superior to many of the commonly used tissue clearing agents and allows for elucidating better quality data from drug screening experiments.