title: ÐÐ·Ð°Ð¸Ð¼Ð¾ÑÐ²ÑÐ·Ñ Ð¼ÐµÐ¶Ð´Ñ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸ÐµÐ¹ Ð¸Ð½ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð¹ ÐºÐ»ÐµÑÐºÐ¸ Ð¸ ÐÐÐ-ÑÐ²ÑÐ·ÑÐ²Ð°ÑÑÐ¸Ð¼Ð¸ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð°Ð¼Ð¸ Ð½ÐµÐ²Ð¸ÑÐ¸Ð¾Ð½Ð½ÑÑ Ð ÐÐ-Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð· phiKZ-ÑÐ¾Ð´ÑÑÐ²ÐµÐ½Ð½ÑÑ ÑÐ°Ð³Ð¾Ð²
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Interrelation between morphology of infected cell and DNA-binding properties of non-virion RNA-polymerases of phages related to phiKZ
Creators: name: Ð¨ÑÑÐ¾Ð², ÐÐ»Ð°Ð´Ð¸Ð¼Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÑÐ¸Ð»Ð¾Ð³ÐµÐ½ÐµÑÐ¸ÐºÐ°
Ð³Ð¸Ð³Ð°Ð½ÑÑÐºÐ¸Ðµ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð¸
Ð¿ÑÐµÐ²Ð´Ð¾-ÑÐ´ÑÐ¾
Ð½ÐµÐ²Ð¸ÑÐ¸Ð¾Ð½Ð½Ð°Ñ Ð ÐÐ-Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð°
ÑÐµÐ³ÑÐ»ÑÑÐ¸Ñ ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸Ð¸
phylogeny
giant bacteriophages
pseudo-nucleus
non-virion RNA polymerase
regulation of transcription

description: ÐÐ¸Ð³Ð°Ð½ÑÑÐºÐ¸Ðµ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð¸, ÑÐ¾Ð´ÑÑÐ²ÐµÐ½Ð½ÑÐµ phiKZ, Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÑÑ ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸Ñ ÑÐ²Ð¾ÐµÐ³Ð¾ Ð³ÐµÐ½Ð¾Ð¼Ð° Ð¿ÑÐ¸ Ð¿Ð¾Ð¼Ð¾ÑÐ¸ Ð´Ð²ÑÑ ÑÐ¾Ð±ÑÑÐ²ÐµÐ½Ð½ÑÑ Ð¼Ð½Ð¾Ð³Ð¾ÑÑÐ±ÑÐµÐ´Ð¸Ð½Ð¸ÑÐ½ÑÑ ÐÐÐ-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼ÑÑ Ð ÐÐ-Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·: Ð²Ð¸ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ (Ð²Ð ÐÐÐ) Ð¸ Ð½ÐµÐ²Ð¸ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ (Ð½Ð²Ð ÐÐÐ). ÐÐ²Ð ÐÐÐ Ð¾ÑÐ²ÐµÑÐ°ÐµÑ Ð·Ð° ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸Ñ Ð¿Ð¾Ð·Ð´Ð½Ð¸Ñ Ð³ÐµÐ½Ð¾Ð². ÐÐ¾-Ð²Ð¸Ð´Ð¸Ð¼Ð¾Ð¼Ñ, ÑÐµÐ³ÑÐ»ÑÑÐ¸Ñ ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸Ð¸ Ð¿Ð¾Ð·Ð´Ð½Ð¸Ñ Ð³ÐµÐ½Ð¾Ð² Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐµÑÑÑ Ð¿Ð¾-ÑÐ°Ð·Ð½Ð¾Ð¼Ñ Ð´Ð»Ñ ÐºÐ°Ð¶Ð´Ð¾Ð³Ð¾ Ð²Ð¸Ð´Ð° Ð³Ð¸Ð³Ð°Ð½ÑÑÐºÐ¸Ñ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð¾Ð². ÐÐ°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³ phiKZ Ð½Ð° Ð¿Ð¾Ð·Ð´Ð½Ð¸Ñ ÑÑÐ°Ð´Ð¸ÑÑ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸ ÑÐ¾ÑÐ¼Ð¸ÑÑÐµÑ Ð²Ð½ÑÑÑÐ¸ Ð¸Ð½ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð¹ ÐºÐ»ÐµÑÐºÐ¸ Ð¿ÑÐµÐ²Ð´Ð¾-ÑÐ´ÑÐ¾, Ð³Ð´Ðµ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐµÑÑÑ ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸Ñ ÑÐ°Ð³Ð¾Ð²Ð¾Ð³Ð¾ Ð³ÐµÐ½Ð¾Ð¼Ð°, Ð² ÑÐ¾ Ð²ÑÐµÐ¼Ñ ÐºÐ°Ðº Ð³ÐµÐ½Ð¾Ð¼ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³ AR9 ÑÐ°ÑÐ¿ÑÐµÐ´ÐµÐ»ÑÐ½ Ð² ÑÐ¸ÑÐ¾Ð·Ð¾Ð»Ðµ ÐºÐ»ÐµÑÐºÐ¸ Ð² ÑÐµÑÐµÐ½Ð¸Ðµ Ð²ÑÐµÐ³Ð¾ Ð»Ð¸ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐ¸ÐºÐ»Ð°. Ð Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ Ð¼Ñ Ð¿ÑÐ¸Ð¼ÐµÐ½Ð¸Ð»Ð¸ Ð¼ÐµÑÐ¾Ð´Ñ Ð±Ð¸Ð¾Ð¸Ð½ÑÐ¾ÑÐ¼Ð°ÑÐ¸ÐºÐ¸ Ð´Ð»Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð³Ð¸Ð¿Ð¾ÑÐµÐ·Ñ Ð¾ Ð½Ð°Ð»Ð¸ÑÐ¸Ð¸ Ð²Ð·Ð°Ð¸Ð¼Ð¾ÑÐ²ÑÐ·Ð¸ ÐÐÐ-ÑÐ²ÑÐ·ÑÐ²Ð°ÑÑÐ¸Ñ ÑÐ²Ð¾Ð¹ÑÑÐ² Ð½Ð²Ð ÐÐÐ, ÐºÐ¾Ð´Ð¸ÑÑÐµÐ¼ÑÑ phiKZ-ÑÐ¾Ð´ÑÑÐ²ÐµÐ½Ð½ÑÐ¼Ð¸ Ð³Ð¸Ð³Ð°Ð½ÑÑÐºÐ¸Ð¼Ð¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð°Ð¼Ð¸, Ð¸ Ð¾ÑÐ¾Ð±ÐµÐ½Ð½Ð¾ÑÑÐµÐ¹ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¸Ð½ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð¹ ÐºÐ»ÐµÑÐºÐ¸. ÐÑÐ» Ð¿ÑÐ¾Ð²ÐµÐ´ÑÐ½ ÑÐ¸Ð»Ð¾Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð°Ð½Ð°Ð»Ð¸Ð· ÑÑÐ±ÑÐµÐ´Ð¸Ð½Ð¸Ñ Ð½Ð²Ð ÐÐÐ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð¾Ð² phiKZ, AR9 Ð¸ Ð±ÐµÐ»ÐºÐ° Ð¿ÑÐµÐ²Ð´Ð¾-ÑÐ´ÑÐ°. Ð ÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð¼ ÑÐ°Ð±Ð¾ÑÑ ÑÑÐ°Ð»Ð¾ Ð¿Ð¾Ð´ÑÐ²ÐµÑÐ¶Ð´ÐµÐ½Ð¸Ðµ ÑÐ°ÑÑÐ¼Ð°ÑÑÐ¸Ð²Ð°ÐµÐ¼Ð¾Ð¹ Ð³Ð¸Ð¿Ð¾ÑÐµÐ·Ñ.
description: Jumbo bacteriophages related to phiKZ transcribe their genome using two of their own multisubunit DNA-dependent RNA polymerases: virion (vRNAP) and non-virion (nvRNAP). NvRNAP is responsible for the transcription of late genes. Apparently, the regulation of transcription of late genes is carried out differently for each species of giant bacteriophages. The bacteriophage phiKZ forms a pseudo-nucleus inside the infected cell at the late stages of infection, where the transcription of the phage genome is carried out, whereas the bacteriophage AR9 genome is distributed in the cytosol of the cell during the entire lytic cycle. In this work, we applied bioinformatics methods to investigate the hypothesis about the presence of a relationship between the DNA-binding properties of nvRNAP encoded by phiKZ-related jumbo bacteriophages and the features of the infected cell morphology. Phylogenetic analysis of nvRNAP subunits of bacteriophages phiKZ, AR9 and pseudo-nucleus protein was carried out. The result of the work was the confirmation of the considered hypothesis.