Additional file 1 of Population structure and insecticide response of Gammarus spp. in agricultural and upstream forested sites of small streams
Creators: name: Schneeweiss, Anke
name: Schreiner, Verena C.
name: Liess, Matthias
name: Röder, Nina
name: Schwenk, Klaus
name: Schäfer, Ralf B.
Publisher: name: figshare
Year: 2024
Subjects: subject: Genetics
subject: FOS: Biological sciences

description: Additional file 1: Table S1. Coordinates in decimal degrees and elevation in metre above sea level of the sampling sites. Table S2. Relative abundance [%] of Gammarus fossarum, Gammarus pulex and Gammarus roeselii at the sampling sites. Table S3. Physicochemical parameters of test medium at test start. Table S4. Physicochemical parameters of test medium at test end. Table S5. Nominal and measured concentrations applied in acute toxicity test setup at test start (0h). Table S6. Model outputs of separate full models for Gammarus spp. mortality or immobility after 96 h exposure to imidacloprid. Table S7. Analysis of deviance table for the generalised linear mixed-effects models (GLMM) listed in Table S6 using type II Wald Chi-squared tests. Table S8. Estimated lethal and effect concentrations in μg/L after 96 h exposure to imidacloprid where 50 % of test organisms (Gammarus spp.) have been affected for each site type. Table S9. Overview of the absolute and relative haplotype frequency as well as variable sites sampled per haplotypes in a fragment of the mitochondrial cytochrome c oxidase subunit one gene (CO1) of Gammarus fossarum. Table S10. Overview table of population genetic metrics for Gammarus fossarum per sampling site. Table S11. Model outputs of separate full models for the haplotype- and nucleotide diversity in a fragment of the mitochondrial cytochrome c oxidase subunit one gene (CO1) of Gammarus fossarum individuals as response to site type. Table S12. Type III analysis of variance table with Kenward–Roger’s method for linear mixed effect models listed in Table S11. Table S13. Pairwise CO1 fixation index (FST) values between all sampling sites. Table S14. Analysis of molecular variance (AMOVA) results for mtDNA CO1 data of Gammarus fossarum individuals from 17 sampling sites (=populations) modelled separately for two different groups (i.e. 6 streamsGammarus fossarum and 3 site types). Table S15. Overview table of population genetic metrics for Gammarus pulex per sampling site. Table S16. Model outputs of separate full models for the lipid content in μg/mg gammarid or the dry weight in mg as response to site type or the abundance of Gammarus fossarum. Table S17. Type III analysis of variance table with Kenward–Roger’s method for linear mixed effect models listed in Table S16. Text S1. Chemical analyses in acute toxicity tests. Text S2. Slightly modified salt-extraction protocol for the extraction of genomic DNA. Text S3. Slightly modified lipid-quantification protocol. Text S4. CO1 sequence data preparation, quality control and taxonomy assignment. Text S5. Lower between stream population differentiation for Gammarus pulex compared to Gammarus fossarum—extended discussion. Figure S1. Stability of the highest toxicity test concentration from start to the end of the test (0–96 h). Figure S2. Concentration–response relationship visualised for the immobility of Gammarus spp. individuals after 96 h of exposure to imidacloprid. Figure S3. Heatmap visualising the genetic differentiation between Gammarus fossarum populations from different sampling sites. Figure S4. Dot plots visualising the total lipid content [μg] normalised by the dry weight per gammarid [mg] (A) and the dry weight per gammarid (B) for the second experimental run for Gammarus fossarum.