title: ÐÑÑÑÐ¾ÑÐµÐ½Ð½ÑÐµ Ð½Ð°ÑÑÑÐµÐ½Ð¸Ñ ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¿Ð»Ð°ÑÑÐ¸ÑÐ½Ð¾ÑÑÐ¸ Ð² Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ðµ ÐºÑÑÑ Ð¿Ð¾ÑÐ»Ðµ ÐµÐ´Ð¸Ð½Ð¸ÑÐ½Ð¾Ð³Ð¾ ÑÐ¿Ð¸Ð·Ð¾Ð´Ð° Ð³ÐµÐ½ÐµÑÐ°Ð»Ð¸Ð·Ð¾Ð²Ð°Ð½Ð½ÑÑ ÑÑÐ´Ð¾ÑÐ¾Ð³
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Delayed violations of synaptic plasticity in the rat hippocampus after a single episode of generalized seizures
Creators: name: Ð¢ÑÐ¾ÑÐ¸Ð¼Ð¾Ð²Ð°, ÐÐ»Ð¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸ÑÐµÑÐºÐ¸Ð¹ ÑÑÐ°ÑÑÑ
Ð¿ÐµÐ½ÑÐ¸Ð»ÐµÐ½ÑÐµÑÑÐ°Ð·Ð¾Ð»Ð¾Ð²Ð°Ñ Ð¼Ð¾Ð´ÐµÐ»Ñ
Ð´Ð¾Ð»Ð³Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð°Ñ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°ÑÐ¸Ñ
Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿
Ð½ÐµÐ¹ÑÐ¾Ð´ÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸Ñ
NMDA-ÑÐµÑÐµÐ¿ÑÐ¾ÑÑ
mGluR1
status epilepticus
pentylenetetrazole seizure model
long-term potentiation
hippocampus
NMDARs

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¾ÑÐ¾Ð±ÐµÐ½Ð½Ð¾ÑÑÐµÐ¹ ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð´Ð¾Ð»Ð³Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð¹ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°ÑÐ¸Ð¸ (ÐÐÐ) Ð¸ Ð¼Ð¾ÑÑÐ¾ÑÑÑÑÐºÑÑÑÐ½ÑÑ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹ Ð² Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ðµ ÐºÑÑÑ ÐÐ¸ÑÑÐ°Ñ ÑÐµÑÐµÐ· Ð¼ÐµÑÑÑ Ð¿Ð¾ÑÐ»Ðµ Ð¿ÐµÑÐµÐ½ÐµÑÐµÐ½Ð½Ð¾Ð³Ð¾ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÑÐ°ÑÑÑÐ° (Ð­Ð¡), Ð²ÑÐ·Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð¿ÐµÐ½ÑÐ¸Ð»ÐµÐ½ÑÐµÑÑÐ°Ð·Ð¾Ð»Ð¾Ð¼ (ÐÐ¢Ð). ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: 1. ÐÐ·ÑÑÐ¸ÑÑ Ð¾ÑÐ¾Ð±ÐµÐ½Ð½Ð¾ÑÑÐ¸ ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÐÐÐ Ð² ÑÐ¸Ð½Ð°Ð¿ÑÐ°Ñ CA3-CA1 Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð° ÐºÑÑÑ ÑÐµÑÐµÐ· 30 Ð´Ð½ÐµÐ¹ Ð¿Ð¾ÑÐ»Ðµ Ð¿ÐµÑÐµÐ½ÐµÑÐµÐ½Ð½Ð¾Ð³Ð¾ Ð­Ð¡ Ð¿ÑÐ¸ Ð´Ð²ÑÑ Ð¿ÑÐ¾ÑÐ¾ÐºÐ¾Ð»Ð°Ñ ÑÑÐ¸Ð¼ÑÐ»ÑÑÐ¸Ð¸; 2. ÐÑÐµÐ½Ð¸ÑÑ ÑÐ¾Ð»Ñ NMDA-ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð² Ð² Ð¸Ð½Ð´ÑÐºÑÐ¸Ð¸ ÐÐÐ Ð² ÑÐ¸Ð½Ð°Ð¿ÑÐ°Ñ CA3-CA1 Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð° ÐºÑÑÑ ÑÐµÑÐµÐ· 30 Ð´Ð½ÐµÐ¹ Ð¿Ð¾ÑÐ»Ðµ ÐÐ¢Ð-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð­Ð¡; 3. ÐÑÐµÐ½Ð¸ÑÑ ÑÐ¾Ð»Ñ GluN2B-ÑÐ¾Ð´ÐµÑÐ¶Ð°ÑÐ¸Ñ NMDA-ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð² Ð² ÑÐ¾ÑÐ¼Ð¸-ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ ÐÐÐ; 4. ÐÑÐµÐ½Ð¸ÑÑ ÑÐ¾Ð»Ñ Ð¼ÐµÑÐ°Ð±Ð¾ÑÑÐ¾Ð¿Ð½ÑÑ Ð³Ð»ÑÑÐ°Ð¼Ð°ÑÐ½ÑÑ ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð² I Ð³ÑÑÐ¿Ð¿Ñ 1-Ð³Ð¾ Ð¿Ð¾Ð´ÑÐ¸Ð¿Ð° Ð² ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ ÐÐÐ Ð² Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ðµ ÐºÑÑÑ; 5. ÐÐ·ÑÑÐ¸ÑÑ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ ÐºÐ¾Ð°Ð³Ð¾Ð½Ð¸ÑÑÐ¾Ð² NMDA-ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð² D-ÑÐµÑÐ¸Ð½Ð° Ð¸ Ð³Ð»Ð¸ÑÐ¸Ð½Ð° Ð½Ð° ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÐÐÐ Ð¿ÑÐ¸ Ð´Ð²ÑÑ Ð¿ÑÐ¾ÑÐ¾ÐºÐ¾Ð»Ð°Ñ ÑÑÐ¸Ð¼ÑÐ»ÑÑÐ¸Ð¸; 6. ÐÑÐµÐ½Ð¸ÑÑ Ð¼Ð¾ÑÑÐ¾ÑÑÑÑÐºÑÑÑÐ½ÑÐµ Ð¾ÑÐ¾Ð±ÐµÐ½Ð½Ð¾ÑÑÐ¸ Ð² Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ðµ ÐºÑÑÑ ÑÐµÑÐµÐ· Ð¼ÐµÑÑÑ Ð¿Ð¾ÑÐ»Ðµ Ð­Ð¡, Ð²ÑÐ·Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ ÐÐ¢Ð. Ð Ð°Ð±Ð¾ÑÐ° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ ÐÐ­Ð¤Ð Ð ÐÐ, Ð² Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½ÑÑ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð¾Ð² Ð½ÐµÐ¹ÑÐ¾Ð½Ð½ÑÑ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ð¹. ÐÐ»Ñ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ Ð¿ÑÐ¸Ð¼ÐµÐ½ÑÐ»Ð°ÑÑ Ð¼ÐµÑÐ¾Ð´Ð¸ÐºÐ° Ð¾ÑÐ²ÐµÐ´ÐµÐ½Ð¸Ñ Ð¿Ð¾Ð»ÐµÐ²ÑÑ Ð²Ð¾Ð·Ð±ÑÐ¶Ð´Ð°ÑÑÐ¸Ñ Ð¿Ð¾ÑÑ-ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð¾Ð² Ð¾Ñ ÑÐ°Ð´Ð¸Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÐ»Ð¾Ñ Ð¿Ð¾Ð»Ñ Ð¡Ð1 Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð°. ÐÐÐ Ð²ÑÐ·ÑÐ²Ð°Ð»Ð¸ ÑÐµÑÐ°-ÑÑÐ¸Ð¼ÑÐ»ÑÑÐ¸ÐµÐ¹ Ð¸ Ð²ÑÑÐ¾ÐºÐ¾ÑÐ°ÑÑÐ¾ÑÐ½Ð¾Ð¹ ÑÑÐ¸Ð¼ÑÐ»ÑÑÐ¸ÐµÐ¹. ÐÐ¾ÑÑÐ¾ÑÑÑÑÐºÑÑÑÐ½ÑÐµ Ð¾ÑÐ¾Ð±ÐµÐ½Ð½Ð¾ÑÑÐ¸ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð° Ð±ÑÐ»Ð¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ñ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¼ÐµÑÐ¾Ð´Ð° Ð¾ÐºÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ñ Ð¿Ð¾ ÐÐ¸ÑÑÐ»Ñ. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð½Ð°Ð¼Ð¸ Ð±ÑÐ»Ð¾ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¾, ÑÑÐ¾ ÑÐµÑÐµÐ· Ð¼ÐµÑÑÑ Ð¿Ð¾ÑÐ»Ðµ Ð­Ð¡ ÐÐÐ Ð¾ÑÐ»Ð°Ð±Ð»ÐµÐ½Ð°, Ð¸ ÑÑÐ¾ ÑÐ¾Ð¿ÑÐ¾Ð²Ð¾Ð¶Ð´Ð°ÐµÑÑÑ Ð½ÐµÐ¹ÑÐ¾Ð´ÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸ÐµÐ¹ Ð² Ð¿Ð¾Ð»Ðµ Ð¡Ð3 Ð¸ ÑÐ¸Ð»Ð»ÑÑÐµ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð°. ÐÑÐ»Ð°Ð±Ð»ÐµÐ½Ð¸Ðµ Ð²ÑÑÐ°Ð±Ð¾ÑÐºÐ¸ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°ÑÐ¸Ð¸ ÑÐ²ÑÐ·Ð°Ð½Ð¾ Ñ Ð½Ð°ÑÑÑÐµÐ½Ð¸ÐµÐ¼ ÑÐ²Ð¾Ð¹ÑÑÐ² Ð¸ Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ NMDA-ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð², Ð²ÑÐ»ÐµÐ´ÑÑÐ²Ð¸Ðµ ÑÐµÐ³Ð¾ ÐÐÐ ÑÑÐ°Ð½Ð¾Ð²Ð¸ÑÑÑ Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾Ð¹ Ð¾Ñ ÑÐ°Ð±Ð¾ÑÑ mGluR1. Ð¢Ð°ÐºÐ¸Ð¼ Ð¾Ð±ÑÐ°Ð·Ð¾Ð¼, Ð¼Ñ Ð²ÑÑÐ²Ð¸Ð»Ð¸ Ð¿ÑÐ¾Ð»Ð¾Ð½Ð³Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐµ Ð½Ð°ÑÑÑÐµÐ½Ð¸Ñ ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¿Ð»Ð°ÑÑÐ¸ÑÐ½Ð¾ÑÑÐ¸, Ð²ÑÐ·Ð²Ð°Ð½Ð½ÑÐµ Ð¾Ð´Ð½Ð¾ÐºÑÐ°ÑÐ½Ð¾ Ð¿ÐµÑÐµÐ½ÐµÑÐµÐ½Ð½ÑÐ¼Ð¸ Ð³ÐµÐ½ÐµÑÐ°Ð»Ð¸Ð·Ð¾Ð²Ð°Ð½Ð½ÑÐ¼Ð¸ ÑÑÐ´Ð¾ÑÐ¾Ð³Ð°Ð¼Ð¸.
description: The given work is devoted to study of features of long-term potentiation (LTP) formation and morphostructural changes in the hippocampus of Wistar rats one month after status epilepticus (SE) caused by pentylenetetrazole (PTZ). The research set the following goals: 1. Studying features of LTP formation in the synapses of CA3-CA1 in rat hippocampus 30 days after SE using 2 stimulation protocols; 2. Evaluating the role of NMDA-receptors in the induction of LTP in rat hippocampal; 3. Evaluating the role of GluN2B-containing NMDA-receptors in the formation of LTP; 4. Studying the role of metabotropic glutamate receptors of group I 1st subtype in LTP formation; 5. Studying the effect of NMDA-receptors co-agonists of D-serine and glycine on LTP formation; 6. Evaluating the morphostructural features in the rat hippocampus one month after SE caused by PTZ. The study was fulfilled on the basis of IEPB RAS, in the laboratory of molecular mechanisms of neural interactions. Extracellular field excitatory postsynaptic potentials were registered from the CA1 stratum radiatum of the hippocampus. LTP was induced by theta-burst stimulation and high-frequency stimulation. The morphostructural features of the hippocampus were investigated using the Nissl staining. As a result, we found that a month after SE, LTP was reduced, and this was accompanied by neurodegeneration in the hippocampus field CA3 and hillus. The attenuation of potentiation is associated with a violation of the properties and localization of NMDA-receptors, as a result of which LTP becomes dependent on the work of mGluR1. Thus, we revealed prolonged synaptic plasticity disorders caused by once-transferred generalized seizures.