Additional file 1 of The extracellular vesicle of gut microbial Paenalcaligenes hominis is a risk factor for vagus nerve-mediated cognitive impairment
Creators: name: Kyung-Eon Lee
name: Jeon-Kyung Kim
name: Sang-Kap Han
name: Lee, Dong Yun
name: Hae-Ji Lee
name: Sung-Vin Yim
name: Kim, Dong-Hyun
Publisher: name: figshare
Year: 2020
Subjects: subject: Biochemistry
subject: Space Science
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Neuroscience
subject: Physiology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: FOS: Chemical sciences
subject: Science Policy
subject: Immunology
subject: FOS: Clinical medicine
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: 111714 Mental Health
subject: FOS: Health sciences
subject: FOS: Health sciences
subject: Hematology
subject: 60506 Virology
subject: Computational Biology

description: Additional file 1: Figure S1. The number of fecal bacterial colonies grown in Bifidobacteria/lactobacilli-selective BL and Enterobacteriaceae-selective DHL agar plates and Paenalcageligenes hominis and Escherichia coli populations in the feces. Figure S2. Effects of young and aged mouse fecal transplantations on the occurrence of cognitive impairment and colitis in the transplanted mice. Figure S3. Intensities of Fig. 1 immunoblotting and confocal microscope data. Figure S4. Effects of Escherichia coli strains isolated from the feces of young adult, elderly individual, and young mice, and aged mice and a Paenalcaligenes hominis strain isolated from the feces of elderly individual on the occurrence of cognitive impairment and colitis in mice. Figure S5. Intensities of Fig. 2 immunoblotting and confocal microscope data. Figure S6. Paenalcaligenes hominis (A) and Escherichia coli (B) dose-dependently caused cognitive impairment in specific pathogen-free mice in Y-maze task. Figure S7. Intensities of Fig. 3 immunoblotting and confocal microscope data. Figure S8. Vagotomy delayed the defecation in mice with (Vx) or without vagotomy (NC). Figure S9. Intensities of Fig. 5 immunoblotting and confocal microscope data. Figure S10. Effects of Paenalcaligenes hominis and Escherichia coli on the gut microbiota composition in mice with or without vagotomy. Figure S11. Intensities of Fig. 6 immunoblotting and confocal microscope data. Figure S12. Effects of Paenalcaligenes hominis extracellular vesicles (EVs) and/or lipopolysaccharide (LPS) on the occurrence of cognitive impairment and colitis in mice with or without vagotomy. Figure S13. Intensities of Figure 7 immunoblotting and confocal microscope data. Figure S14. Intensities of Figure 8A confocal microscope data. Figure S15. Paenalcaligenes hominis and Escherichia coli on the expression of GABA and NMDA receptors in the hippocampus. Figure S16. Transmission electron microscope image of Paennalcaligenes hominis (PH) extracellular vesicle (EV). Figure S17. Sodium-polyacrylamide gel electrophoresis of intact PH and EV (A, B, and C, shown in Table S1). Figure S18. Protocols of in vivo experiments. Figure S19. Accumulated effects of Paenalcaligenes hominis with or without vagotomy and Escherichia coli with or without vagotomy on the occurrence of cognitive impairment in the Y-maze task. Table S1. LC-MS-MS data of EV A, B, and C proteins. Table S2. Clinical characteristics of study participants. Table S3. Primers for the qPCR of Escherichia coli and Paenalcaligenes hominis.Table S4. Primers for qPCR. Table S5. P values of experimental data. Methods 1. Mouse breeding and gut bacteria Paenalcaligenes hominis and Escherichia coli assay. 2. Purification of LPS from Paenalcaligenes hominis (PH). 3. Properties of extracellular vesicles purified from Paennalcaligenes hominis (PH). 4. Quantitative real time – polymerase chain reaction (qPCR) for GABA receptors