title: ÐÐ°Ð»Ð¸Ð´Ð°ÑÐ¸Ñ Ð½Ð°Ð±Ð¾ÑÐ° Ð´Ð»Ñ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ ÐÐÐ Ð²Ð¸ÑÑÑÐ½Ð¾Ð³Ð¾ Ð³ÐµÐ¿Ð°ÑÐ¸ÑÐ° Ð Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ ÐÐ¦Ð Ð² ÑÐµÐ°Ð»ÑÐ½Ð¾Ð¼ Ð²ÑÐµÐ¼ÐµÐ½Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Validation of the kit for determining the DNA of the hepatitis B virus by real-time PCR
Creators: name: ÐÐµÑÑÐ°ÑÐ¸Ð½Ð°, ÐÐ°ÑÐ³Ð°ÑÐ¸ÑÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Ð²Ð¸ÑÑÑ Ð³ÐµÐ¿Ð°ÑÐ¸ÑÐ° Ð
Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð½Ð°Ñ ÑÐµÐ¿Ð½Ð°Ñ ÑÐµÐ°ÐºÑÐ¸Ñ (ÐÐ¦Ð )
Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐ°
hepatitis b virus
polymerase chain reaction (PCR)
diagnosis

description: ÐÐ° 74 Ñ., 26 ÑÐ¸ÑÑÐ½ÐºÐ¾Ð², 33 ÑÐ°Ð±Ð»Ð¸ÑÑ, 12 Ð¿ÑÐ¸Ð»Ð¾Ð¶ÐµÐ½Ð¸Ð¹.Ð¦ÐµÐ»Ñ ÑÐ°Ð±Ð¾ÑÑ â Ð¾ÑÐµÐ½ÐºÐ° ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÑÐ°Ð±Ð¾ÑÑ ÐÐ¦Ð -Ð½Ð°Ð±Ð¾ÑÐ° Â«ÐÐ½ÑÐ¸ÑÐ¸ÐºÐ° ÐÐÐÂ» (Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´Ð¸ÑÐµÐ»Ñ: ÐÐ âÐÐ»ÐºÐ¾Ñ ÐÐ¸Ð¾â, Ð Ð¾ÑÑÐ¸Ñ) Ð´Ð»Ñ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð²Ð¸ÑÑÑÐ° Ð³ÐµÐ¿Ð°ÑÐ¸ÑÐ° Ð Ð¿ÑÐ¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ð¸ ÑÑÑÑÑ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´Ð¸ÑÐµÐ»ÐµÐ¹.ÐÐ°Ð´Ð°ÑÐ¸:1. ÐÐ·ÑÑÐµÐ½Ð¸Ðµ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÑ Ð¿Ð¾ ÑÐµÐ¼Ðµ ÑÐ°Ð±Ð¾ÑÑ;2. ÐÐ¾Ð´Ð±Ð¾Ñ ÑÐ¾ÑÑÐ°Ð²Ð° ÑÐ¼ÐµÑÐ¸ Ð´Ð»Ñ Ð¿Ð¾ÑÑÐ°Ð½Ð¾Ð²ÐºÐ¸ ÐÐ¦Ð -Ð Ð.ÐÑÐ¾Ð²ÐµÑÐºÐ° ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾Â­ÑÑÐ¸ ÑÐ°Ð±Ð¾ÑÑ ÑÐ¸ÑÑÐµÐ¼Ñ. ÐÑÐ¾Ð²ÐµÑÑÐ¸ Ð·Ð°Ð¼ÐµÐ½Ñ ÐºÐ¾Ð¼Ð¿Ð¾Ð½ÐµÐ½ÑÐ¾Ð² ÐÐ¦Ð -ÑÐ¼ÐµÑÐ¸: ÐÐ¦Ð -Ð±ÑÑÐµÑ, Ð¼Ð°Ð³Ð½Ð¸Ð¹, dNTPâs (Ð´ÐµÐ·Ð¾ÐºÑÐ¸Ð½ÑÐºÐ»ÐµÐ¾ÑÐ¸Ð´ÑÑÐ¸ÑÐ¾ÑÑÑÐ°ÑÑ), Ð¿ÑÐ°Ð¹Ð¼ÐµÑÑ (Ñ ÑÐ°Ð·Ð½ÑÐ¼Ð¸ Ð·Ð¾Ð½Ð´Ð°Ð¼Ð¸), Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ñ;3. ÐÐ¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ñ ÐÐ¦Ð -ÑÐ¼ÐµÑÐ¸ Ð¿ÑÑÐµÐ¼ Ð´Ð¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸Ñ ÑÐµÑÐ¼ÐµÐ½ÑÐ° Ð£ÐÐÂ (ÑÑÐ°ÑÐ¸Ð»Ð´ÐµÐ³Ð¸Ð´ÑÐ¾Ð³ÐµÐ½Ð°Ð·Ñ);4. ÐÐ¿ÑÐ¾Ð±Ð°ÑÐ¸Ñ Ð½Ð°Ð±Ð¾ÑÐ¾Ð² Ð½Ð° Ð°Ð¼Ð¿Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¾ÑÐµ Light Cycler;5. ÐÐ½Ð°Ð»Ð¸Ð· Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð².Ð ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð¾ÑÐ½Ð¾Ð²Ð½Ð¾Ð³Ð¾ Ð¼ÐµÑÐ¾Ð´Ð° Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐ¸ Ð±ÑÐ»Ð° Ð²ÑÐ±ÑÐ°Ð½Ð° ÐÐ¦Ð  Ð² ÑÐµÐ°Ð»ÑÐ½Ð¾Ð¼ Ð²ÑÐµÐ¼ÐµÐ½Ð¸.Â  ÐÐ±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¸Ðµ ÐÐÐ Ð²Ð¸ÑÑÑÐ° Ð³ÐµÐ¿Ð°ÑÐ¸ÑÐ° Ð Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÐµÑ ÑÐ²Ð¾ÐµÐ²ÑÐµÐ¼ÐµÐ½Ð½Ð¾ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÑÐ¾Ð²Ð°ÑÑ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ðµ Ð¸ Ð¿ÑÐµÐ´Ð¾ÑÐ²ÑÐ°ÑÐ¸ÑÑ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½ÑÐµ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÐ²Ð¸Ñ. Ð¦ÐµÐ»ÑÑ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÐ»ÑÑ Ð¿Ð¾Ð´Ð±Ð¾Ñ ÐºÐ¾Ð¼Ð¿Ð¾Ð½ÐµÐ½ÑÐ¾Ð² ÐÐ¦Ð -ÑÐ¼ÐµÑÐ¸, ÑÑÐ¾ Ð°ÐºÑÑÐ°Ð»ÑÐ½Ð¾ Ð¿ÑÐ¸ ÑÐµÑÐµÐ½Ð¸Ð¸ Ð²Ð¾Ð¿ÑÐ¾ÑÐ° Ð¸Ð¼Ð¿Ð¾ÑÑÐ¾Ð·Ð°Ð¼ÐµÑÐµÐ½Ð¸Ñ.
description: 74 pages, 26 figures, 33 tables, 12 appendices.The purpose of the work is to evaluate the effectiveness of the PCR kit "Intifica HBV" (manufacturer: GC "Alcor Bio", Russia) for the detection of hepatitis B virus using raw materials from different manufacturers.Tasks:1. Study of literature on the topic of work;2. Selection of the composition of the mixture for setting PCR-RT, checking the efficiency of the system. Change the components of the PCR mixture: PCR buffer, magnesium, dNTP's (deoxynucleotide triphosphates), primers (with different probes), polymerases;3. Modification of the PCR mixture by adding the enzyme UDH (uracil dehydrogenase);4. Approbation of the kits on the Light Cycler;5. Analysis of the results.Real-time PCR was chosen as the main diagnostic method. Detection of hepatitis B virus DNA allows timely diagnosis of the disease and prevention of possible consequences. The aim of the work was to select the components of the PCR mixture, which is relevant when solving the issue of import substitution.