title: ÐÐ½Ð°Ð»Ð¸Ð· Ð´Ð°Ð½Ð½ÑÑ Ð¼Ð¸Ð³ÑÐ°ÑÐ¸Ð¸ Ð¿Ð»ÑÑÐ¸Ð¿Ð¾ÑÐµÐ½ÑÐ½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Analysis of human pluripotent cell migration data
Creators: name: Ð¨ÑÐ°Ð³Ð¸Ð½, ÐÐ²Ð³ÐµÐ½Ð¸Ð¹
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: ÐºÐ»Ð°ÑÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸ÑÂ ÐºÐ»ÐµÑÐ¾Ðº Ð¸ ÐºÐ¾Ð»Ð¾Ð½Ð¸Ð¹
Ð¿Ð»ÑÑÐ¸Ð¿Ð¾ÑÐµÐ½ÑÐ½ÑÐµ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°
ÑÐ¸ÑÐ»ÐµÐ½Ð½ÑÐµ Ñ Ð°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ¸ Ð´Ð²Ð¸Ð¶ÐµÐ½Ð¸Ñ ÐºÐ¾Ð»Ð¾Ð½Ð¸Ð¹
ÑÐ¸Ð¿ Ð´Ð¸ÑÑÑÐ·Ð¸Ð¸
classification of cells and colonies
human pluripotent stem cells
numerical characteristics of colony movement
type of diffusion

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description: The subject of the graduate qualification work is "Analysis of human pluripotent cell migration data". The paper investigates the features of migration of human pluripotent cell colonies in experiments on their cultivation. Analysis of the movement of cell colonies allows us to understand the mechanisms of collective behavior of cells in growing colonies. Methods of colony motion analysis were studied and the dynamics of several migration parameters were calculated, including total distance, net distance, root-mean-square displacement, meandering index, coverage coefficient, instantaneous velocity, velocity autocorrelation, total angle of rotation and direction autocorrelation. The calculated migration parameters were compared between colonies of the H9 cell line with two genotypes â the wild type and the mutant line by a gene associated with molecular mechanisms that ensure cell mobility. As a result of the analysis of migration parameters, statistically significant differences in the nature of movement between the two genotypes were identified. The migration data of wild-type colonies were investigated within the framework of two diffusion models. As a result of the analysis of the dynamics of the rootâmean-square displacement, it is shown that the colonies demonstrate a normal type of diffusion corresponding to the Ornstein-Uhlenbeck process. The correlation of morphological parameters of colonies (diameter and roundness) with colony migration parameters is analyzed. It is shown that of all the experiments conducted, there is only one weak relationship between the parameters - between the diameter and the overall angle of rotation, in all other cases there is no statistically significant relationship. The results obtained clarify the nature of the collective behavior of human pluripotent cells during the cultivation of cell colonies for different genotypes.