Additional file 1 of Quantitative CT imaging and radiation-absorbed dose estimations of 166Ho microspheres: paving the way for clinical application
Creators: name: Morsink, Chiron
name: Klaassen, Nienke
name: van de Maat, Gerrit
name: Boswinkel, Milou
name: Arranja, Alexandra
name: Bruggink, Robin
name: van Houwelingen, Ilva
name: Schaafsma, Irene
name: Hesselink, Jan Willem
name: Nijsen, Frank
name: van Nimwegen, Bas
Publisher: name: figshare
Year: 2024
Subjects: subject: Biophysics
subject: Space Science
subject: Physical Sciences not elsewhere classified
subject: Medicine
subject: Cell Biology
subject: Physiology
subject: FOS: Biological sciences
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Mathematical Sciences not elsewhere classified
subject: Cancer

description: Additional file 1: Supplementary Table S1. 166Ho-MS injection and quantification parameters in veterinary patients. Supplementary Table S2. 166Ho-MS injection and quantification parameters in phantoms (n = 5). Supplementary Table S3. Planned and measured holmium (Ho) concentrations in a Ho chloride (Ho-Cl) phantom and a Ho poly(L-lactic) acid microspheres (Ho-MS) phantom with corresponding CT calibration measurement results consisting of the maximum (max.) and mean Hounsfield Unit (HU) values with the standard deviation (SD) for two CT scanners and one combination of acquisition parameters. The exposures (mAs) between the two scanners were set based on the matched CTDIvol. The Ho concentrations in the Ho-Cl phantom were determined using Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES) and in the Ho-MS phantom by weighing the necessary amounts of Ho-MS upon preparation. The measured HU values on CT were used to plot CT calibration curves and to calculate the corresponding intercept and slope values with regression statistics (Fig. 5). *Maximum HU of scanner reached (3071 HU for Siemens scanner). NA = Not applicable. Supplementary Table 4. CT acquisition parameters used for CT calibration for holmium using two different scanners (Siemens SOMATOM Definition AS and Canon Aquilion ONE). Exposures (mAs) were set on the Siemens scanner and the resulting CTDIvol (mGy) values per setting were used on the Canon scanner to obtain equal exposures with respect to the scanned volumes, for proper comparison of measured HU values. Supplementary Table 5. CT calibration results for detection of holmium (Ho) based on Hounsfield Unit (HU) measurements in a Ho (III) chloride hexahydrate (Ho-Cl3) phantom and a Ho poly(L-lactic) acid microspheres (Ho-PLLA-MS) phantom both containing multiple Ho concentrations (n). CT scans were acquired using a Siemens SOMATOM Definition AS (top) and a Canon Aquilion One (bottom) scanner. Each row represents one dataset acquired by a combination of different acquisition parameters: tube kilovoltage peak (kVp), exposure (mAs)/CTDIvol (mGy), slice thickness (mm), reconstructed using two soft-tissue kernels, and one bone kernel. The Ho concentrations in the Ho-Cl3 phantom reached the maximum HU value of the Siemens scanner (HU of 3,071), and we included the maximum (Max.) Ho concentration (mg/mL) that could be detected before this max. HU value was reached, together with the number of concentrations/measurement points (n) that were included to calculate the calibration intercept (m) and slope (b) values with their standard error (SE). Statistics of the regression are also included: R-squared (R2) values, the F-statistic (F) of the ANOVA test with the degrees of freedom (df), and p-values of the coefficients. Supplementary Table 6. Descriptive statistics and test results for holmium-166 microspheres injections, quantification, recovery, and volume fractions after injection in phantoms and veterinary patients.