Diatrype mangrovei Dayarathne &amp; K. D. Hyde 2020, sp. nov.
Creators: name: Dayarathne, Monika C.
name: Wanasinghe, Dhanushka N.
name: Devadatha, B.
name: Abeywickrama, Pranami
name: G, E. B.
name: Jones, areth
name: Chomnunti, Putarak
name: Sarma, V. V.
name: Hyde, Kevin D.
name: Lumyong, Saisamorn
name: C., Eric H.
name: , Mckenzie
Publisher: name: Zenodo
Year: 2020
Subjects: Biodiversity
Taxonomy
Fungi
Ascomycota
Dothideomycetes
Pleosporales
Teichosporaceae
Diatrype
Diatrype mangrovei

description: <i>Diatrype mangrovei</i> Dayarathne &amp; K.D.Hyde, sp. nov. (Fig. 12) MYCOBANK. — MB 82430. FACESOFFUNGI NUMBER. — FoF 03949. ETYMOLOGY. — Referring to the common name of the host ecosystem. C ULTURE CHARACTERISTICS. — Colonies on PDA reaching 5 cm diam., after two weeks at 20-25°C, medium dense, irregular, flat, surface smooth with undulate edge, colony from above yellow at the margin, greenish yellow at the centre; from below yellowish white at the margin, greenish yellow at the centre; mycelium greenish yellow, not produced pigmentation. MATERIAL EXAMINED. — <b>Thailand</b>, Ranong Province, Amphoe Muang, Mu 4 Tambol Ngao, Ranong Mangrove Research Center (9°43’- 9°57’N, 98°29’- 98°39’E), on fallen decaying twig of <i>Bruguiera cylindrica</i>, 6.XII.2016, <i>Monika C. Dayarathne</i>, MCD 067 (holo-, MFLU 17-0412), ex-type living culture, MFLUCC 17-0412, ICMP 21769; on fallen decaying twig of <i>B. cylindrica</i>, 6.XII.2016, <i>Monika C. Dayarathne</i>, MCD 030 (iso-, MFLU 18-0147), ex-type living culture, MFLUCC 17-0391; on fallen decaying twig of <i>B. cylindrica</i>, 6.XII.2016, <i>Monika C. Dayarathne</i>, MCD 034 (MFLU 18-0148), ex-type living culture, MFLUCC 17-0394. DISTRIBUTION. — Thailand DESCRIPTION Saprobic on submerged decaying wood of <i>Bruguiera cylindrica</i>. <i>Sexual morph</i> <b>Ascostromata.</b> 1-2 × 0.8-1 cm, scattered or aggregated on host, erumpent, arising through cracks on the bark, edges of ascostromata remaining as pointed, angular parts, carbonaceous, with numerous perithecia immersed in one stroma. <b>Ostiole.</b> Opening through host bark and appearing as black spots, composed of an outer layer of dark brown, small, tightly packed, thin parenchymatous cells and an inner layer of yellowish white, large, loosely packed, parenchymatous cells. <b>Ascomata.</b> 480-500 × 300-340 µm (ẍ = 490 × 320 µm, n = 0), perithecia immersed in stromatic tissue, aggregated, brown, globose to sub-globose, narrowing towards the apex and very narrow at the base of ostiolar canal, thin-walled, ostiolate; ostiolar canal, periphysate, ostiolar opening covered with carbonaceous, black cells; periphyses hyaline, filamentous. <b>Peridium.</b> 40-45 µm wide, comprising an outer layer of yellow-brown, thick-walled cells of textura angularis and a thin, inner stratum of yellow, thick-walled cells of textura angularis. <b>Asci.</b> 90-120 × 6-8 µm (ẍ = 105 × 7 µm, n = 30), unitunicate, 8-spored, with long, narrow, thin-walled stalk, with cylindrical, thick-walled, swollen upper portion, apex flat, with J-, cylindrical, conspicuous apical ring. <b>Ascospores.</b> 5-10.5 × 1.5-2.5 µm (ẍ = 7.5 × 2 µm, n =30), seriate, hyaline becoming yellowish at maturity, allantoid, unicellular, thin-walled, with small fat globules at each end, smooth-walled. <i>Asexual morph</i> Undetermined. NOTES <i>Diatrype mangrovei</i> Dayarathne &amp; K.D.Hyde, sp. nov., was recorded from intertidal attached wood of <i>Bruguiera cylindrica</i> from Ranong, Thailand. In the phylogenetic analyses of both ITS and concatenated ITS+Btub data (Figs 1; 2) it grouped in a clade with <i>Diatrype</i> and <i>Diatrypella</i> species. <i>Diatrype mangrovei</i> Dayarathne &amp; K.D.Hyde, sp. nov., shares similar morphological features with the genus <i>Diatrype</i> and is distinguished from <i>Diatrypella</i> species by being octosporous. <i>Diatrype enteroxantha</i> (HUEFS155114, HUEFS155116) showed close phylogenetic affinity to this new species (subclade A 4 in clade A, Fig. 1) but <i>D. enteroxantha</i> has smaller asci (18-28.5 × 5-9 µm vs 90-120 × 6-8 µm) (de Almeida <i>et al.</i> 2016). There are 14 bp differences out of 520 bp (2.69%) between <i>D. enteroxantha</i> and <i>D. mangrovei</i> Dayarathne &amp; K.D.Hyde, sp. nov., According to ITS phylogeny, <i>D. mangrovei</i> Dayarathne &amp; K.D.Hyde, sp. nov., formed an intermediate clade (sub-clade L 4 in clade L) to <i>D. enteroxantha</i> and <i>D. oregonensis</i> (DPL200) (Fig. 2). <i>Diatrype mangrovei</i> Dayarathne &amp; K.D.Hyde, sp. nov., is easily distinguishable from <i>D. oregonensis</i> by having globose to sub-globose ascomata while the latter species has ovoid or ellipsoid ascomata with a white entostroma that becomes yellow to brown (Trouillas <i>et al.</i> 2010a). However, there are 16 bp are differences out of 526 bp (3.04%) in ITS region between <i>D. oregonensis</i> and <i>D. mangrovei</i> Dayarathne &amp; K.D.Hyde, sp. nov., Morphological differences of <i>D. mangrovei</i> Dayarathne &amp; K.D.Hyde, sp. nov., and related taxa are summarized in the Table 5. This is the first record of <i>Diatrype</i> species from a mangrove habitat.
description: Published as part of <i>Dayarathne, Monika C., Wanasinghe, Dhanushka N., Devadatha, B., Abeywickrama, Pranami, G, E. B., Jones, areth, Chomnunti, Putarak, Sarma, V. V., Hyde, Kevin D., Lumyong, Saisamorn, C., Eric H. &amp; Mckenzie, 2020, Modern taxonomic approaches to identifying diatrypaceous fungi from marine habitats, with a novel genus Halocryptovalsa Dayarathne &amp; K. D. Hyde, gen. nov., pp. 21-67 in Cryptogamie, Mycologie 20 (3)</i> on pages 46-48, DOI: 10.5252/cryptogamie-mycologie2020v41a3, http://zenodo.org/record/7815042
description: {"references": ["DE ALMEIDA D. A. C., GUSMAO L. F. P. &amp; MILLER A. N. 2016. - Taxonomy and molecular phylogeny of Diatrypaceae (Ascomycota, Xylariales) species from the Brazilian semi-arid region, including four new species. Mycological Progress 15: 1 - 27. https: // doi. org / 10.1007 / s 11557 - 016 - 1194 - 8", "TROUILLAS F. P., URBEZ- TORRES J. R. &amp; GUBLER W. D. 2010 a. - Diversity of diatrypaceous fungi associated with grapevine canker diseases in California. Mycologia 102: 319 - 336. https: // doi. org / 10.3852 / 08 - 185"]}