title: ÐÐµÑÐ¾Ð´Ñ Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐ´ÐµÑ Ð² ÑÑÐ¸ÑÑÐ¾ÑÐ¸ÑÐ°Ñ Ð¿ÐµÑÐ¸ÑÐµÑÐ¸ÑÐµÑÐºÐ¾Ð¹ ÐºÑÐ¾Ð²Ð¸ Ð¼Ð»ÐµÐºÐ¾Ð¿Ð¸ÑÐ°ÑÑÐ¸Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Methods for studying micronuclei in mammalian peripheral blood erythrocytes
Creators: name: ÐÐ¸ÑÐºÐ¸Ð½Ð°, ÐÐºÐ°ÑÐµÑÐ¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Ð¼Ð¸ÐºÑÐ¾ÑÐ´ÑÐ°
ÑÑÐ¸ÑÑÐ¾ÑÐ¸ÑÑ
Ð³ÐµÐ½Ð¾ÑÐ¾ÐºÑÐ¸ÑÐ½Ð¾ÑÑÑ
Ð¼Ð¸ÐºÑÐ¾ÑÐ´ÐµÑÐ½ÑÐ¹ ÑÐµÑÑ
Ð±Ð¸Ð¾Ð¼Ð¾Ð½Ð¸ÑÐ¾ÑÐ¸Ð½Ð³
Ð¿ÑÐ¾ÑÐ¾ÑÐ½Ð°Ñ ÑÐ¸ÑÐ¾Ð¼ÐµÑÑÐ¸Ñ
Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ
micronuclei
micronucleus assay
rbc
genotoxity
biomonitoring
flow cytometry
microscopy

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description: The topic of the final qualifying work: "Methods for studying micronuclei in mammalian peripheral blood erythrocytes". The paper presents the theoretical part concerning genotoxicity and the history of micronucleus research using Russian and foreign literature. During the execution of the work, the following tasks were set: 1. Collect and analyze literature data, using domestic and foreign sources, in the field of micronucleus research and genotoxicity research. 2. To estimate the number of polychromatophilic erythrocytes, normochromatophilic erythrocytes, micronuclei in polychromatophilic erythrocytes and normochromatophilic erythrocytes in peripheral blood of mice by microscopy and analyze the same blood on a flow cytometer. 3. Compare the micronucleus accounting data obtained by flow cytometry and microscopy. The work was carried out on the basis of RMC Â«HOME OF PHARMACYÂ», where the material was collected and analyzed: microscopic analysis of peripheral blood samples and analysis of the same blood on a flow cytometer. The necessary calculations were carried out and a statistical analysis of the experiments was given. The method of studying micronuclei is presented, in particular, two research methods are analyzed: the classical method of manual counting using electron microscopy and automation of the method using flow cytometry.