title: Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð¼ÐµÑÐ¾Ð´Ð¾Ð² ÑÑÐ¾ÑÐ½ÐµÐ½Ð¸Ñ ÑÐµÐ³Ð¼ÐµÐ½ÑÐ°ÑÐ¸Ð¸ ÑÐ¾ÑÐ¼Ñ Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÑ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð² Ð´Ð»Ñ Ð¸Ñ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐµÐ¹ ÐºÐ»Ð°ÑÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Development of refinement methods of dendritic spines form segmentation for further classification
Creators: name: ÐÐ°Ð²Ð»Ð¾Ð², ÐÐ»ÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÐµ ÑÐ¸Ð¿Ð¸ÐºÐ¸
ÑÐµÐ³Ð¼ÐµÐ½ÑÐ°ÑÐ¸Ñ
ÐºÐ»Ð°ÑÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ñ
Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÐ° Ð¸Ð·Ð¾Ð±ÑÐ°Ð¶ÐµÐ½Ð¸Ð¹
dendritic spines
segmentation
classification
image processing

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ ÑÑÑÐµÑÑÐ²ÑÑÑÐ¸Ñ Ð¿Ð¾Ð´ÑÐ¾Ð´Ð¾Ð² Ðº ÑÐµÐ³Ð¼ÐµÐ½ÑÐ°ÑÐ¸Ð¸ ÑÑÑÑÐ¼ÐµÑÐ½ÑÑ Ð¸Ð·Ð¾Ð±ÑÐ°Ð¶ÐµÐ½Ð¸Ð¹ Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÑ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð² Ð¸ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐµ Ð¼ÐµÑÐ¾Ð´Ð° ÑÑÐ¾ÑÐ½ÐµÐ½Ð¸Ñ ÑÐµÐ³Ð¼ÐµÐ½ÑÐ°ÑÐ¸Ð¸ Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÑ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð². Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½ Ð°Ð²ÑÐ¾Ð¼Ð°ÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð°Ð»Ð³Ð¾ÑÐ¸ÑÐ¼ ÑÐµÐ³Ð¼ÐµÐ½ÑÐ°ÑÐ¸Ð¸ ÑÐµÐµÐº Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÑ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð². Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð½ÑÐ¹ Ð°Ð»Ð³Ð¾ÑÐ¸ÑÐ¼ Ð¾ÑÐ½Ð¾Ð²Ð°Ð½ Ð½Ð° Ð¿Ð¾ÑÑÑÐ¾ÐµÐ½Ð¸Ð¸ Ð³ÑÐ°ÑÐ° Ð¸Ð·Ð¾Ð±ÑÐ°Ð¶ÐµÐ½Ð¸Ñ Ð¸ Ð¿Ð¾Ð¸ÑÐºÐµ Ð¿ÑÑÐ¸ Ñ Ð½Ð°Ð¸Ð¼ÐµÐ½ÑÑÐµÐ¹ ÑÑÐ¾Ð¸Ð¼Ð¾ÑÑÑÑ Ð¼ÐµÐ¶Ð´Ñ ÐºÐ»ÑÑÐµÐ²ÑÐ¼Ð¸ ÑÐ¾ÑÐºÐ°Ð¼Ð¸. ÐÐ¾Ð¸ÑÐº ÐºÐ»ÑÑÐµÐ²ÑÑ ÑÐ¾ÑÐµÐº Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐµÑÑÑ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¸Ð·Ð²Ð»ÐµÑÐµÐ½Ð¸Ñ ÑÐºÐµÐ»ÐµÑÐ° ÑÑÐ²Ð¾Ð»Ð° Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ°. ÐÐ°Ð¹Ð´ÐµÐ½Ð½ÑÐ¹ Ð¿ÑÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·ÑÐµÑÑÑ ÐºÐ°Ðº Ð½Ð°ÑÐ°Ð»ÑÐ½ÑÐ¹ ÐºÐ¾Ð½ÑÑÑ Ð´Ð»Ñ ÑÐµÐ³Ð¼ÐµÐ½ÑÐ°ÑÐ¸Ð¸ ÑÐµÐ¹ÐºÐ¸ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð°Ð»Ð³Ð¾ÑÐ¸ÑÐ¼Ð° ACWE (Active Contours Without Edges) Ñ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ð¼Ð¸ Ð¾Ð¿ÐµÑÐ°ÑÐ¸ÑÐ¼Ð¸. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐ¹ Ð°Ð»Ð³Ð¾ÑÐ¸ÑÐ¼ Ð²ÑÑÑÐ¾ÐµÐ½ Ð² ÑÑÑÐµÑÑÐ²ÑÑÑÐµÐµ Ð¿ÑÐ¸Ð»Ð¾Ð¶ÐµÐ½Ð¸Ðµ. Ð¡ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¾Ð±Ð½Ð¾Ð²Ð»ÑÐ½Ð½Ð¾Ð¹ Ð²ÐµÑÑÐ¸Ð¸ Ð¿ÑÐ¸Ð»Ð¾Ð¶ÐµÐ½Ð¸Ñ Ð±ÑÐ»Ð¸ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐ°Ð½Ñ 30 Ð¸Ð·Ð¾Ð±ÑÐ°Ð¶ÐµÐ½Ð¸Ð¹ ÐºÐ¾Ð½ÑÐ¾ÐºÐ°Ð»ÑÐ½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐ¹ Ð½Ð°Ð±Ð¾Ñ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð² Ð±ÑÐ» Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½ Ð´Ð»Ñ Ð¾Ð±ÑÑÐµÐ½Ð¸Ñ SVM-ÐºÐ»Ð°ÑÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¾ÑÐ°. ÐÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ðµ ÑÐºÐ¾ÑÐ¾ÑÑÐ¸ Ð¸ ÐºÐ°ÑÐµÑÑÐ²Ð° ÑÐµÐ³Ð¼ÐµÐ½ÑÐ°ÑÐ¸Ð¸ Ð² Ð½Ð¾Ð²Ð¾Ð¹ Ð¸ Ð¸ÑÑÐ¾Ð´Ð½Ð¾Ð¹ Ð²ÐµÑÑÐ¸Ð¸ Ð¿ÑÐ¸Ð»Ð¾Ð¶ÐµÐ½Ð¸Ñ. ÐÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ðµ ÐºÐ°ÑÐµÑÑÐ²Ð° ÐºÐ»Ð°ÑÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Ð½Ð° Ð½Ð°Ð±Ð¾ÑÐ°Ñ Ð´Ð°Ð½Ð½ÑÑ, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð´Ð²ÑÑ Ð²ÐµÑÑÐ¸Ð¹ Ð¿ÑÐ¸Ð»Ð¾Ð¶ÐµÐ½Ð¸Ñ.
description: This work is focused on examination of existing approaches to the segmentation of three-dimensional dendritic spines images and development of method for dendritic spines segmentation refinement. Auotomated algorithm for dendritic spines necks segmentation was developed. The developed algorithm is based on building image graph and finding a minimum cost path between key points. The key points are found by extracting the dendritic shaft skeleton. Found path is used as initial contour for segmentation with morphological version of ACWE (Active Contours Without Edges) algorithm. The developed algorithm was integrated into existing application. 30 confocal microscopy images were processed using the updated application. Obtained dataset was used to fit SVM classification. Segmentation speed and quality in the new and original versions of the application were compared. Classification quality was compared on datasets obtained with both versions of the application.