title: ÐÐ¾Ð»ÑÑÐµÐ½Ð¸Ðµ Ð¸ ÑÑÐ°ÐºÑÐ¸Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð»Ð¸Ð¿Ð¸Ð´Ð¾Ð² Ð¸Ð· Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Extraction and fractionation of lipids from the microalgae biomass
Creators: name: Ð¡Ð¾ÐºÐ¾Ð»Ð¾Ð²ÑÐºÐ°Ñ, ÐÐ¸Ð´Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÐÐ¸Ð¾ÑÐµÑ Ð½Ð¾Ð»Ð¾Ð³Ð¸Ñ
ÐÐ¸Ð¿Ð¸Ð´Ñ
ÑÐºÑÑÑÐ°ÐºÑÐ¸Ñ
Ð¿Ð¾Ð»Ð¸Ð½ÐµÐ½Ð°ÑÑÑÐµÐ½Ð½ÑÐµ Ð¶Ð¸ÑÐ½ÑÐµ ÐºÐ¸ÑÐ»Ð¾ÑÑ
ÑÑÐ°ÐºÑÐ¸Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
extraction
polyunsaturated fatty acids
fractionation

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð¿Ð¾ ÑÐµÐ¼Ðµ: Â«ÐÐ¾Ð»ÑÑÐµÐ½Ð¸Ðµ Ð¸ ÑÑÐ°ÐºÑÐ¸Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð»Ð¸Ð¿Ð¸Ð´Ð¾Ð² Ð¸Ð· Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹Â». ÐÑÐµÐ´Ð¼ÐµÑÐ¾Ð¼ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÑÑÑÑ ÑÐ¸Ð·Ð¸ÐºÐ¾-ÑÐ¸Ð¼Ð¸ÑÐµÑÐºÐ¸Ðµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹ Chlorella vulgaris BIN Ð¸ Nannochloropsis BJ17. Ð¦ÐµÐ»Ñ ÑÐ°Ð±Ð¾ÑÑ â Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ðµ ÑÑÐ°Ð²Ð½Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð¼ÐµÑÐ¾Ð´Ð¾Ð² ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸ Ð»Ð¸Ð¿Ð¸Ð´Ð¾Ð² (Ð¡Ð¾ÐºÑÐ»ÐµÑ Ð¸ ÑÐ»ÑÑÑÐ°Ð·Ð²ÑÐºÐ¾Ð²Ð¾Ð¹ Ð¼ÐµÑÐ¾Ð´), ÑÑÐ°ÐºÑÐ¸Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð»Ð¸Ð¿Ð¸Ð´Ð¾Ð² Ñ ÑÐµÐ»ÑÑ Ð¾ÑÐ¸ÑÐµÐ½Ð¸Ñ ÐÐÐÐ. Ð ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½Ð¾Ð¹ ÑÐ°ÑÑÐ¸ ÑÐ°ÑÑÐ¼Ð¾ÑÑÐµÐ½Ñ Ð²Ð°ÑÐ¸Ð°Ð½ÑÑ ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸ Ð»Ð¸Ð¿Ð¸Ð´Ð¾Ð² Ð¸Ð· Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹ C. vulgaris BIN, Nannochloropsis BJ17 Ð¿ÑÐ¸ Ð¿Ð¾Ð¼Ð¾ÑÐ¸ Ð°Ð¿Ð¿Ð°ÑÐ°ÑÐ° Ð¡Ð¾ÐºÑÐ»ÐµÑÐ° Ð¸ ÑÐ»ÑÑÑÐ°Ð·Ð²ÑÐºÐ¾Ð²Ð¾Ð³Ð¾ Ð¼ÐµÑÐ¾Ð´Ð°, Ð¿Ð¾Ð´Ð¾Ð±ÑÐ°Ð½Ñ ÑÑÐ»Ð¾Ð²Ð¸Ñ ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÑ, Ð²ÑÐµÐ¼ÐµÐ½Ð¸ ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸, ÑÐ°ÑÑÐ²Ð¾ÑÐ¸ÑÐµÐ»Ð¸, Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½ Ð²ÑÑÐ¾Ð´ Ð»Ð¸Ð¿Ð¸Ð´Ð¾Ð², Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½ ÑÑÐ°Ð²Ð½Ð¸ÑÐµÐ»ÑÐ½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð·. ÐÐ»Ñ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ ÑÑÐ°Ð²Ð½Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð¶Ð¸ÑÐ½Ð¾ÐºÐ¸ÑÐ»Ð¾ÑÐ½Ð¾Ð³Ð¾ ÑÐ¾ÑÑÐ°Ð²Ð° Ð±ÑÐ»Ð¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ñ Ð¤ÑÑÑÐµ-ÐÐ-ÑÐ¿ÐµÐºÑÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ Ð¸ Ð³Ð°Ð·Ð¾Ð²Ð°Ñ ÑÑÐ¾Ð¼Ð°ÑÐ¾Ð³ÑÐ°ÑÐ¸Ñ. ÐÑÑÐ¾Ð´ Ð»Ð¸Ð¿Ð¸Ð´Ð¾Ð² ÑÐ¾ÑÑÐ°Ð²Ð¸Ð» Ð±Ð¾Ð»ÐµÐµ 11 % Ð¿ÑÐ¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ð¸ ÑÐ¸ÑÑÐµÐ¼Ñ ÑÐ°ÑÑÐ²Ð¾ÑÐ¸ÑÐµÐ»ÐµÐ¹ Ð³ÐµÐºÑÐ°Ð½: ÑÑÐ¸Ð»Ð¾Ð²ÑÐ¹ ÑÐ¿Ð¸ÑÑ. ÐÑÐ» Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½ Ð¶Ð¸ÑÐ½Ð¾ÐºÐ¸ÑÐ»Ð¾ÑÐ½ÑÐ¹ ÑÐ¾ÑÑÐ°Ð² Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð»Ð¸Ð¿Ð¸Ð´Ð¾Ð² Ñ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐ¸Ð¼ ÑÑÐ°ÐºÑÐ¸Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸ÐµÐ¼ ÐºÐ°ÑÐ±Ð°Ð¼Ð¸Ð´Ð½ÑÐ¼ ÑÐ¿Ð¾ÑÐ¾Ð±Ð¾Ð¼. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ, Ð±ÑÐ»Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ñ Ð´Ð²Ð° Ð¿ÑÐ¾Ð´ÑÐºÑÐ° Ñ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÐ¼ Ð½Ð°Ð±Ð¾ÑÐ¾Ð¼ Ð¶Ð¸ÑÐ½ÑÑ ÐºÐ¸ÑÐ»Ð¾Ñ: ÐºÐ°ÑÐ±Ð°Ð¼Ð¸Ð´Ð¾ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ½Ð°Ñ ÑÑÐ°ÐºÑÐ¸Ñ Ñ Ð¼Ð¾Ð½Ð¾Ð½ÐµÐ½Ð°ÑÑÑÐµÐ½Ð½ÑÐ¼Ð¸ Ð¸ Ð½Ð°ÑÑÑÐµÐ½Ð½ÑÐ¼Ð¸ Ð¶Ð¸ÑÐ½ÑÐ¼Ð¸ ÐºÐ¸ÑÐ»Ð¾ÑÐ°Ð¼Ð¸, Ð¸ Ð¾ÑÐ½Ð¾ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾ Ð¾ÑÐ¸ÑÐµÐ½Ð½ÑÐ¹ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°Ñ ÐÐÐÐ, Ð½ÐµÐºÐ°ÑÐ±Ð°Ð¼Ð¸Ð´Ð¾ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ½Ð°Ñ ÑÑÐ°ÐºÑÐ¸Ñ, Ñ Ð½ÐµÐ·Ð°Ð¼ÐµÐ½Ð¸Ð¼ÑÐ¼Ð¸ Ð»Ð¸Ð½Ð¾Ð»ÐµÐ²Ð¾Ð¹, Ð°Ð»ÑÑÐ°-Ð»Ð¸Ð½Ð¾Ð»ÐµÐ½Ð¾Ð²Ð¾Ð¹, ÑÐ¹ÐºÐ¾Ð·Ð°Ð¿ÐµÐ½ÑÐ°ÐµÐ½Ð¾Ð²Ð¾Ð¹ Ð¸ Ð´Ð¾ÐºÐ¾Ð·Ð°Ð³ÐµÐºÑÐ°ÐµÐ½Ð¾Ð²Ð¾Ð¹ Ð¶Ð¸ÑÐ½ÑÐ¼Ð¸ ÐºÐ¸ÑÐ»Ð¾ÑÐ°Ð¼Ð¸. ÐÐ½Ð°Ð»Ð¸Ð· ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð² ÑÑÐ°ÐºÑÐ¸Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð»Ð¸Ð¿Ð¸Ð´Ð½Ð¾Ð³Ð¾ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ° Ð±ÑÐ» Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½ Ð² ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²Ð¸Ð¸ Ñ ÐÐÐ¡Ð¢ 31663-2012, ÐÐÐ¡Ð¢ 31665-2012 Ð¸ ÐÐÐ¡Ð¢ Ð  ÐÐ¡Ð 5508-2010.
description: This thesis is fulfilled on the topic: âExtraction and fractionation of lipids from the microalgae biomassâ. The subject of this thesis is the physicochemical properties of Chlorella vulgaris BIN and Nannochloropsis BJ17 microalgae. The purpose of the thesis is to conduct a comparative analysis of lipid extraction methods (Soxlet and ultrasonic method), lipid fractionation in order to purify PUFA. The experimental part considers the options for extracting lipids from C. vulgaris BIN, Nannochloropsis BJ17 microalgae using the Soxlet apparatus and the ultrasonic extraction method. The temperature conditions, extraction time, solvents were selected, the lipid yield was determined, and a comparative analysis was carried out. Fourier-IR spectroscopy and gas chromatography were performed to conduct a comparative analysis of the fatty acid composition. The lipid yield was more than 11% when using the solvent system hexane: ethyl alcohol. The fatty acid composition of the obtained lipids was determined with subsequent fractionation by the urea method. As a result, two products with a different set of fatty acids were obtained: the urea fraction with monounsaturated and saturated fatty acids, and a relatively purified PUFA concentrate, a non-urea fraction, with essential linoleic, alpha-linolenic, eicosapentaenoic and docosahexaenoic fatty acids. The analysis of lipid complex fractionation was performed in accordance with GOST 31663-2012, GOST 31665-2012 and GOST R ISO 5508-2010.