Additional file 1 of Extracellular vesicles from hiPSC-NSCs can prevent peripheral inflammation-induced cognitive dysfunction with inflammasome inhibition and improved neurogenesis in the hippocampus
Creators: name: Ayyubova, Gunel
name: Kodali, Maheedhar
name: Upadhya, Raghavendra
name: Madhu, Leelavathi N.
name: Attaluri, Sahithi
name: Somayaji, Yogish
name: Shuai, Bing
name: Rao, Shama
name: Shankar, Goutham
name: Shetty, Ashok K.
Publisher: name: figshare
Year: 2024
Subjects: subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Molecular Biology
subject: Neuroscience
subject: Physiology
subject: FOS: Biological sciences
subject: Pharmacology
subject: Immunology
subject: FOS: Clinical medicine
subject: Cancer
subject: Mental Health
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Computational Biology

description: Additional file 1: Figure S1. Human induced pluripotent stem cells derived neural stem cells (hIPSC-NSCs) express specific markers. Images A-D illustrate that all cells in the passage 11 NSCs derived from hiPSCs express NSC markers Nestin (A) and Sox-2 (B). Scale bar, 100 μm. Figure S2. Seven days of Lipopolysaccharide (LPS) administration induced systemic inflammation and neuroinflammation when examined a day after the last LPS injection. The bar charts A-J compare the concentrations of proinflammatory cytokines in the liver (A-D), serum (E-H), and hippocampus (I-L), such as tumor necrosis factor-alpha (TNF-α, A, E, I); interleukin beta (IL-1β, B, F, J); IL-6 (DC, G, K); and IL-18 (D, H, L). *, p &lt; 0.05; **, p &lt; 0.01; ***, p &lt; 0.001; NS, not significant. Figure S3. Intranasally administered hiPSC-NSC-EVs incorporated into NeuN + neurons and in multiple brain regions of lipopolysaccharide (LPS) treated mice. A-L: Images illustrating the incorporation of EVs into NeuN+ neurons in the medial prefrontal cortex (mPFC; A, B), the somatosensory cortex (SSC; C, D), dentate granule cell layer (GCL; E, F), the CA1 cell layer (G, H), the CA3 cell layer (I, J) and midbrain (MB; K, L) in LPS-treated mice 6 hours post-administration. The images in B, D, F, H, J, and L represent the magnified versions of images A, C, E, G, I, and K indicated in boxes. Scale bar-A-L, 12.5 μm. Figure S4. Intranasally administered hiPSC-NSC-EVs incorporated into IBA-1+ microglia in multiple brain regions of lipopolysaccharide (LPS) treated mice. A-L: Images illustrating the incorporation of EVs into IBA-1+ microglia in the medial prefrontal cortex (mPFC; A, B), the somatosensory cortex (SSC; C, D), dentate granule cell layer (GCL; E, F), the CA1 cell layer (G, H), the CA3 cell layer (I, J) and midbrain (MB; K, L) in LPS-treated mice 6 hours post-administration. The images in B, D, F, H, J, and L represent the magnified versions of images A, C, E, G, I, and K indicated in boxes. Scale bar-A-L, 12.5 μm. Figure S5. Intranasally administered hiPSC-NSC-EVs incorporated into GFAP+ astrocytes in the hippocampus of lipopolysaccharide (LPS)-treated mice. The figure illustrates the incorporation of EVs into GFAP + astrocytes in the CA1 (A) and CA3 (B) subfields of the hippocampus of Lipopolysaccharide (LPS) treated mice at 6 hours post-administration—scale bar, 12.5 μm. Figure S6. Additional data of the object-in-place test (OIPT), temporal pattern processing task (TPPT), and pattern separation test (PST). The bar charts A-B compare the total object exploration times (TOETs) in T2 and T3 across groups in OIPT. The bar charts M-P compare the TOETs in T2–T5 across groups in TPPT. The bar charts G-H compare the TOETs in T2 and T3. The bar charts in G-I compare the TOETs in T2–T4 across groups in PST. *, p &lt; 0.05; **, p &lt; 0.01; NS, not significant. Figure S7. (Blots with corresponding inverts for EV markers shown in Fig. 1). Figure S8. (Blots with corresponding original membrane for EV markers shown in Fig. 1). Figure S9. (Blots with corresponding inverts for synaptophysin and PSD95 shown in Fig. 11). Figure S10. (Blots with corresponding original membrane for synaptophysin and PSD95 shown in Fig. 11.