Additional file 2 of Coexistence patterns of soil methanogens are closely tied to methane generation and community assembly in rice paddies
Creators: name: Li, Dong
name: Ni, Haowei
name: Jiao, Shuo
name: Lu, Yahai
name: Zhou, Jizhong
name: Sun, Bo
name: Liang, Yuting
Publisher: name: figshare
Year: 2021
Subjects: subject: Environmental Sciences not elsewhere classified
subject: Ecology
subject: FOS: Biological sciences
subject: Biological Sciences not elsewhere classified
subject: Infectious Diseases
subject: FOS: Health sciences

description: Additional file 1: Figure S1. Sampling and sequencing of methanogens. Abscissa: the number of sequences randomly selected from the sample; ordinate: the species diversity and the number of OTU that can be represented by the random sampling sequence. Each curve in the figure represents a sample, marked with a different color. Figure S2. Linear regression of methanogenic archaea α-diversity and MAT (A) and CH4 emissions (B). Solid lines denote significant relationship with 95% confidence interval (shadow area). Dotted line denotes P &gt; 0.05. Figure S3. The co-occurrence network structure of methanogenic community of 39 plots. Percentage in the top right corner indicates the proportion of 33 key OTUs (Table S4) in total node numbers of each network. Figure S4. Contributions of climatic variables and soil properties to the network topological attributes based on correlation and random forest regression model. Circle size represents the variable importance (that is, proportion of explained variability calculated via forest regression analysis analysis). Colors represent Spearman’s correlation coefficients (node: node number; edge: edge number; modularity: modularity; positive: positive correlations; negative: negative correlations; Avg.C.C: average clustering coefficient; N.D: network diameter; C.P.L: characteristic path length). Figure S5. Spearman’s correlation analysis of network topological attributes and richness. Blue shaded area is a 95% confidence interval. Figure S6. Spearman’s correlation analysis of network topological attributes and CH4 emissions. Dotted lines denote P &gt; 0.05. Solid lines indicate a significant correlation (P &lt; 0.05), with the blue shaded area showing the 95% confidence interval. Figure S7. Relative abundance of OTUs of the five groups of methanogenic archaeal communities. Each column followed by different letters differed significantly at P &lt; 0.05 (ANOVA, Duncan test). (A.endemic: always endemic group; C.endemic: conditionally endemic group; C.common: conditionally common group; A.common: always common group). Figure S8. Relative abundance frequency histogram of 33 keystone OTUs in 429 samples. The blue histogram indicates rare OTUs (average relative abundance &lt; 1% in 429 samples). The red histogram indicates abundant OTUs (average relative abundance &gt; 1% in 429 samples), and the inset represents low-frequency OTUs with average relative abundance &lt; 1%. Figure S9. The relative abundance of keystone taxa (at the family level) changes with temperature. (HL: 1.5°C, CC: 4.5°C, SY:8.3°C, YY: 14.4°C, FQ:13.9°C, LA: 16.1°C, QZ:17.9°C, ZX:17°C, JO:18.8°C, CT:19°C, HY:18°C, QX:21.6°C, HK:23.8°C). Figure S10. The relationship between keystone species and microbial functional genes related to methane generation depicted as colored segments in a CIRCOS plot. Ribbons connecting two segments indicate the interaction between the two. The size of the ribbon is proportional to the number of links. Table S1. Geographic information of 13 sampling regions with 3 plots in each region. Table S2. The topological features of the co-occurrence network in each plot. Table S3. Partial mantel test between network topology attributes and variables. Table S4. Changes of network topology attributes with latitude. Table S5. Information of 33 keystone OTUs involved in common co-occurrence relationship.