title: Ð¢ÐµÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ ÑÐ°Ð½Ð½Ð¸Ñ Ð³ÐµÐ½Ð¾Ð² Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð° phiKZ Ð½Ð° ÑÐ¾ÑÑ Ð³ÑÐ°Ð¼Ð¾ÑÑÐ¸ÑÐ°ÑÐµÐ»ÑÐ½ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Testing the effect of the expression of early phiKZ bacteriophage genes on the growth of gram-negative bacteria
Creators: name: Ð¡Ð°Ð³Ð°Ð¹Ð´Ð°Ðº, ÐÐºÐ°ÑÐµÑÐ¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³
phiKZ
ÑÐ°Ð½Ð½Ð¸Ðµ Ð³ÐµÐ½Ñ
Pseudomonas aeruginosa
Escherichia coli
bacteriophage
early genes

description: ÐÐ°Ð´Ð°ÑÐ¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: â ÐÐ»Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°ÑÑ ÑÐ°Ð½Ð½Ð¸Ðµ Ð³ÐµÐ½Ñ gp260, gp261, gp262, gp263 Ð¸ gp283 ÑÐ°Ð³Ð° phiKZ Ð² ÑÐ°ÑÑÐ»-Ð²ÐµÐºÑÐ¾Ñ pHERD20T. â ÐÐ·ÑÑÐ¸ÑÑ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ ÑÐ°Ð½Ð½Ð¸Ñ Ð³ÐµÐ½Ð¾Ð² Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð° phiKZ Ð½Ð° Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÑÐ¾ÑÑÐ° Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ Pseudomonas aeruginosa Ð¸ Escherichia coli Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¿Ð»Ð°Ð½ÑÐµÑÐ½Ð¾Ð³Ð¾ ÑÐ¿ÐµÐºÑÑÐ¾ÑÐ¾ÑÐ¾Ð¼ÐµÑÑÐ°. ÐÐ±ÑÐµÐºÑÐ¾Ð¼ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÑÑÑÑ ÑÐ°Ð½Ð½Ð¸Ðµ Ð³ÐµÐ½Ñ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð° phiKZ (gp260, gp261, gp262, gp263, gp283). ÐÑÐµÐ´Ð¼ÐµÑÐ¾Ð¼ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐ²Ð»ÑÐµÑÑÑ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð¿ÑÑÐ¸ ÑÐ°Ð½Ð½Ð¸Ñ Ð³ÐµÐ½Ð¾Ð² ÑÐ°Ð³Ð° Ð½Ð° ÑÐ¾ÑÑ Ð³ÑÐ°Ð¼Ð¾ÑÑÐ¸ÑÐ°ÑÐµÐ»ÑÐ½ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ Pseudomonas aeruginosa Ð¸ Escherichia coli. ÐÑÐ¸ Ð½Ð°Ð¿Ð¸ÑÐ°Ð½Ð¸Ð¸ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±Ð·Ð¾ÑÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ: eLIBRARY.RU, PubMed. Ð¡ÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÑÑ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»Ð¸ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿Ð°ÐºÐµÑÐ° Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼ Microsoft Excel. Ð Ð°Ð±Ð¾ÑÐ° Ð±ÑÐ»Ð° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ ÐÐÐ Â«ÐÐ°Ð½Ð¾ÐÐ¸Ð¾Â» Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¾Ð³Ð¾ Ð³Ð¾ÑÑÐ´Ð°ÑÑÑÐ²ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑÐ° ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾. Ð ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¿Ð¾Ð»ÑÑÐµÐ½Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¾Ð½Ð½ÑÐµ Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð´Ñ Ð´Ð»Ñ Ð´Ð²ÑÑ ÑÐ°Ð½Ð½Ð¸Ñ Ð³ÐµÐ½Ð¾Ð² â gp260 Ð¸ gp283, Ð´Ð»Ñ Ð¾ÑÑÐ°Ð»ÑÐ½ÑÑ ÑÑÐµÑ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¾Ð½Ð½ÑÐµ Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð´Ñ Ð¿Ð¾Ð»ÑÑÐ¸ÑÑ Ð½Ðµ ÑÐ´Ð°Ð»Ð¾ÑÑ, Ð½ÐµÑÐ¼Ð¾ÑÑÑ Ð½Ð° Ð¼Ð½Ð¾Ð³Ð¾ÐºÑÐ°ÑÐ½ÑÐµ Ð¿Ð¾Ð²ÑÐ¾ÑÑ. ÐÐ´Ð½Ð¾Ð¹ Ð¸Ð· Ð¿ÑÐ¸ÑÐ¸Ð½, Ð¿Ð¾ÑÐµÐ¼Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¾Ð½Ð½ÑÐµ Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð´Ñ Ñ ÑÐ°Ð½Ð½Ð¸Ð¼Ð¸ Ð³ÐµÐ½Ð°Ð¼Ð¸ gp261, gp262, gp263 Ð½Ðµ Ð¿Ð¾Ð»ÑÑÐ¸Ð»Ð¾ÑÑ Ð½Ð°ÑÐ°Ð±Ð¾ÑÐ°ÑÑ, ÑÐ²Ð»ÑÐµÑÑÑ Ð¸Ñ Ð²ÐµÑÐ¾ÑÑÐ½Ð°Ñ ÑÐ¾ÐºÑÐ¸ÑÐ½Ð¾ÑÑÑ Ð´Ð»Ñ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº. Ð­ÐºÑÐ¿ÑÐµÑÑÐ¸Ñ ÑÐ°Ð½Ð½Ð¸Ñ Ð³ÐµÐ½Ð¾Ð² gp260 Ð¸ gp283 Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð° phiKZ Ð½Ðµ Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÐµÑ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð½Ð° ÑÐ¾ÑÑ Ð³ÑÐ°Ð¼Ð¾ÑÑÐ¸ÑÐ°ÑÐµÐ»ÑÐ½ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº E.coli. Ð Ð¾ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð¹ ÐºÑÐ»ÑÑÑÑÑ P.aeruginosa Ð½Ðµ Ð·Ð°Ð²Ð¸ÑÐ¸Ñ Ð¾Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ ÑÐ°Ð½Ð½ÐµÐ³Ð¾ Ð³ÐµÐ½Ð° gp283. ÐÐ´Ð½Ð°ÐºÐ¾ Ð²ÑÑÐ²Ð»ÐµÐ½Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð½Ð° ÑÐ¾ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐºÑÐ¿ÑÐµÑÑÐ¸ÐµÐ¹ Ð³ÐµÐ½Ð° gp260.
description: The tasks of the study: â To clone the early genes gp260, gp261, gp262, gp263 and gp283 of phage phiKZ into the pHERD20T shuttle-vector. â To study the effect of the expression of early phiKZ bacteriophage genes on the inhibition of the growth of Pseudomonas aeruginosa and Escherichia coli bacteria using a flatbed spectrophotometer. The object of this study is the early genes of the bacteriophage phiKZ (gp260, gp261, gp262, gp263, gp283). The subject of the study is the effect of the expression of five early phage genes on the growth of gram-negative bacteria Pseudomonas aeruginosa and Escherichia coli. When writing the literary review, the following databases were used: eLibrary.RU, PubMed. Statistical processing of experimental data was carried out using the Microsoft Excel software package. The work was carried out in the analytical center of nano- and biotechnologies of Peter the Great St. Petersburg Polytechnic University. During the study, expression plasmids were obtained for two early genes gp260 and gp283, for the remaining three expression plasmids could not be obtained, despite repeated repetitions. One of the reasons why expression plasmids with early gp261, gp262, gp263 genes could not be developed is their probable toxicity to bacterial cells. The expression of the early genes gp260 and gp283 of the phiKZ bacteriophage has no effect on the growth of gram-negative bacterial cells of E.coli. The growth of the bacterial culture of P.aeruginosa does not depend on the expression of the early gp283 gene. However, the effect of gp260 gene expression on cell growth was revealed.