title: Role of non-specific weak interaction in PML-bodies formation
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Ð Ð¾Ð»Ñ ÑÐ»Ð°Ð±ÑÑ Ð½ÐµÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ð¹ Ð² Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ð¸ PML-ÑÐµÐ»ÐµÑ
Creators: name: ÐÐµÐ´ÐµÐ»ÑÐµÐ², ÐÐ²Ð³ÐµÐ½Ð¸Ð¹
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: ÐÐµÐ»ÐºÐ¸
ÐÐ¸Ð¾ÑÐµÑ Ð½Ð¾Ð»Ð¾Ð³Ð¸Ñ
Ð½ÐµÐ¼ÐµÐ¼Ð±ÑÐ°Ð½Ð½ÑÐµ Ð¾ÑÐ³Ð°Ð½ÐµÐ»Ð»Ñ
ÑÐ°Ð·Ð¾Ð²ÑÐµ Ð¿ÐµÑÐµÑ Ð¾Ð´Ñ ÑÐ¸Ð¿Ð° Â«Ð¶Ð¸Ð´ÐºÐ¾ÑÑÑ-Ð¶Ð¸Ð´ÐºÐ¾ÑÑÑÂ»
ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ñ
PML-ÑÐµÐ»ÑÑÐ°
C-ÐºÐ¾Ð½ÑÐµÐ²Ð¾Ð¹ Ð´Ð¾Ð¼ÐµÐ½
Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐ° Ð¾Ð±Ð¼ÐµÐ½Ð°
Ð²Ð¾ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¸Ðµ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸
membranelles organelles
liquid-liquid phase transitions
fluorescence
PML bodies
C-terminal domain
exchange dynamics
fluorescence recovery

description: PML-ÑÐµÐ»ÑÑÐ° â ÑÐ´ÐµÑÐ½ÑÐµ Ð½ÐµÐ¼ÐµÐ¼Ð±ÑÐ°Ð½Ð½ÑÐµ Ð¾ÑÐ³Ð°Ð½ÐµÐ»Ð»Ñ, Ð¿ÑÐ¸ÑÑÑÑÑÐ²ÑÑÑÐ¸Ðµ Ð¿ÑÐ°ÐºÑÐ¸ÑÐµÑÐºÐ¸ Ð²Ð¾ Ð²ÑÐµÑ ÑÐ¸Ð¿Ð°Ñ ÐºÐ»ÐµÑÐ¾Ðº Ð¸ Ð¿ÑÐ¸Ð½Ð¸Ð¼Ð°ÑÑ ÑÑÐ°ÑÑÐ¸Ðµ Ð²Ð¾ Ð¼Ð½Ð¾Ð³Ð¸Ñ Ð¿ÑÐ¾ÑÐµÑÑÐ°Ñ: Ð¾Ñ Ð¿Ð¾ÑÑÑÑÐ°Ð½ÑÐ»ÑÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Ð±ÐµÐ»ÐºÐ¾Ð² Ð¸ ÑÐµÐ¿Ð°ÑÐ°ÑÐ¸Ð¸ ÐÐÐ Ð´Ð¾ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ Ð¸ Ð°Ð¿Ð¾Ð¿ÑÐ¾Ð·Ð°, Ð° ÑÐ°ÐºÐ¶Ðµ ÑÐ²Ð»ÑÑÑÑÑ ÑÐµÐ³ÑÐ»ÑÑÐ¾ÑÐ°Ð¼Ð¸ Ð¿ÑÐ¾ÑÐµÑÑÐ¾Ð² Ð°Ð»ÑÑÐµÑÐ½Ð°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÐ´Ð»Ð¸Ð½ÐµÐ½Ð¸Ñ ÑÐµÐ»Ð¾Ð¼ÐµÑ Ð² ÑÐ°ÐºÐ¾Ð²ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ. ÐÑÐ½Ð¾Ð²Ð½ÑÐ¼ ÐºÐ¾Ð¼Ð¿Ð¾Ð½ÐµÐ½ÑÐ¾Ð¼ PML-ÑÐµÐ»ÐµÑ ÑÐ²Ð»ÑÐµÑÑÑ Ð±ÐµÐ»Ð¾Ðº Ð¿ÑÐ¾Ð¼Ð¸ÐµÐ»Ð¾ÑÐ¸ÑÐ°ÑÐ½Ð¾Ð³Ð¾ Ð»ÐµÐ¹ÐºÐ¾Ð·Ð° (PML), ÑÑÑÐµÑÑÐ²ÑÑÑÐ¸Ð¹ Ð² Ð½ÐµÑÐºÐ¾Ð»ÑÐºÐ¸Ñ Ð¸Ð·Ð¾ÑÐ¾ÑÐ¼Ð°Ñ, Ð¸Ð· ÐºÐ¾ÑÐ¾ÑÑÑ Ð¾ÑÐ½Ð¾Ð²Ð½ÑÐ¼Ð¸ ÑÐ²Ð»ÑÑÑÑÑ Ð¿ÐµÑÐ²ÑÐµ ÑÐµÐ¼Ñ (PML-I â PML-VII). Ð Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ Ð±ÑÐ»Ð¾ Ð¸Ð·ÑÑÐµÐ½Ð¾ Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ðµ PML-ÑÐµÐ»ÐµÑ Ð¿ÐµÑÐ²ÑÐ¼Ð¸ Ð¿ÑÑÑÑ Ð¸Ð·Ð¾ÑÐ¾ÑÐ¼Ð°Ð¼Ð¸ PML Ð¸ Ð¸Ñ C-ÐºÐ¾Ð½ÑÐµÐ²ÑÐ¼Ð¸ Ð´Ð¾Ð¼ÐµÐ½Ð°Ð¼Ð¸ Ð² ÐºÐ»ÐµÑÐºÐ°Ñ HeLa Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ Ð½Ð°Ð»Ð¸ÑÐ¸Ñ Ð¸ Ð¾ÑÑÑÑÑÑÐ²Ð¸Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ ÑÐ½Ð´Ð¾Ð³ÐµÐ½Ð½ÑÑ PML. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ ÑÐ²Ð¸Ð´ÐµÑÐµÐ»ÑÑÑÐ²ÑÑÑ Ð¾ ÑÐ¾Ð¼, ÑÑÐ¾ C-ÐºÐ¾Ð½ÑÐµÐ²ÑÐµ Ð´Ð¾Ð¼ÐµÐ½Ñ Ð±ÐµÐ»ÐºÐ¾Ð² PML-II Ð¸ PML-V ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ñ ÐºÐ°Ðº Ð²ÐºÐ»ÑÑÐ°ÑÑÑÑ Ð² PML-ÑÐµÐ»ÑÑÐ°, Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð½ÑÐµ ÑÐ½Ð´Ð¾Ð³ÐµÐ½Ð½ÑÐ¼Ð¸ Ð¸Ð·Ð¾ÑÐ¾Ñ-Ð¼Ð°Ð¼Ð¸ PML, ÑÐ°Ðº Ð¸ ÑÐ°Ð¼Ð¾ÑÑÐ¾ÑÑÐµÐ»ÑÐ½Ð¾ ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°ÑÑ Ð¶Ð¸Ð´ÐºÐ¾-ÐºÐ°Ð¿ÐµÐ»ÑÐ½ÑÐµ ÑÑÑÑÐºÑÑÑÑ Ð² ÑÐ´ÑÐ°Ñ Ð½Ð¾ÐºÐ°ÑÑÐ½ÑÑ Ð¿Ð¾ PML ÐºÐ»ÐµÑÐºÐ°Ñ HeLa. ÐÐ°ÑÑÑÐµÐ½Ð¸Ðµ ÑÐ°Ð¹ÑÐ¾Ð² ÑÑÐ¼Ð¾Ð¸Ð»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð²ÐµÐ´ÑÑ Ðº Ð¿ÑÐ°ÐºÑÐ¸ÑÐµÑÐºÐ¸ Ð¿Ð¾Ð»Ð½Ð¾Ð¼Ñ Ð¾ÑÑÑÑÑÑÐ²Ð¸Ñ Ð½ÐµÐ¼Ð¾Ð±Ð¸Ð»ÑÐ½Ð¾Ð¹ ÑÑÐ°ÐºÑÐ¸Ð¸ PML Ð² ÑÐµÐ»Ñ-ÑÐ°Ñ, Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð½ÑÑ Ð¡-ÐºÐ¾Ð½ÑÐµÐ²ÑÐ¼Ð¸ Ð´Ð¾Ð¼ÐµÐ½Ð°Ð¼Ð¸ PML-II Ð¸ PML-V. ÐÑÐ¸Ð²ÑÐµ FRAP, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÐµ Ð¿ÑÐ¸ Ð°Ð½Ð°Ð»Ð¸Ð·Ðµ Ð²Ð¾ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¸Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸ EGFP Ð² ÐºÐ»ÐµÑÐºÐ°Ñ, Ð¿Ð¾Ð´Ð²ÐµÑÐ¶ÐµÐ½Ð½ÑÑ Ð¾ÐºÐ¸ÑÐ»Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð¼Ñ ÑÑÑÐµÑÑÑ, Ð³Ð¾Ð²Ð¾ÑÑÑ Ð¾ Ð²ÑÑÐ¾ÐºÐ¾Ð¼ Ð²Ð»Ð¸ÑÐ½Ð¸Ð¸ ÑÑÐ¼Ð¾Ð¸Ð»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ C-ÐºÐ¾Ð½ÑÐµÐ²ÑÑ Ð´Ð¾Ð¼ÐµÐ½Ð¾Ð² PML-II Ð¸ PML-V Ð½Ð° ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ¸ Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð½ÑÑ Ð¸Ð¼Ð¸ ÐºÐ¾Ð½Ð´ÐµÐ½ÑÐ°ÑÐ¾Ð². ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ Ð´Ð°Ð½Ð½ÑÐµ Ð³Ð¾Ð²Ð¾ÑÑÑ Ð¾ Ð·Ð½Ð°ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ð¼ Ð²ÐºÐ»Ð°Ð´Ðµ ÑÐ»Ð°Ð±ÑÑ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ð¹ Ð¼ÐµÐ¶Ð´Ñ C-ÐºÐ¾Ð½ÑÐµÐ²ÑÐ¼Ð¸ Ð´Ð¾Ð¼ÐµÐ½Ð°Ð¼Ð¸ PML Ð² Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ðµ PML-ÑÐµÐ»ÐµÑ.
description: PML nuclear bodies are nuclear membrane-less organelles that are present in almost all cell types and are involved in many cell-processes: from post-translational modification of proteins and DNA repair to cell senescence and apop-tosis to appearing as localizers of alternative telomere elongation processes in can-cer cells. The main component of PML bodies is the promyelocytic leukemia (PML) protein, which exists in several isoforms, of which the first seven are the main ones (PML-I to PML-VII). Formation of PML bodies by isoforms PML-I-V in wild-type HeLa cells and PML-knockout HeLa cells and inclusion of their C-terminal domain into endogenous PML bodies were studied. PML-II and PML-V C-terminal domains are able to not only be incorporated into endogenous PML bodies, but also form liquid-drop structures by itself. SUMOylation site disruption causes diffusion of C-terminal domains of PML-II and PML-V in nucleoplasm and almost complete absence of immobile fraction in structures, formed by them. In most cases cute oxidative stress negatively affects the exchange dynamics of PML-bodies. In general, obtained data says that weak nonspecific interactions between intrinsically disordered C-termini of PML have a significant effect on PML nuclear bodies formation.