cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
A Preliminary Survey and Assessment of Plant Communities of the Calcareous Tertiary of Mississippi and Alabama
Creators: name: Kees, John
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2023

description: 
	Mississippi’s Jackson Prairie and related limestone barrens of southwest Alabama are among the most imperiled and poorly studied ecosystems in the Eastern U.S. and a critical component of the North American Coastal Plain biodiversity hotspot. I here offer a preliminary and qualitative classification of plant communities associated with Eocene- and Oligocene- aged calcareous outcrops in the region and an annotated checklist of associated species. A GIS map of “prairie” patches extant within the past 30 years was constructed from satellite imagery, with reference to USDA soils data, and geologic shapefiles. All available georeferenced herbarium records, natural heritage program Element Occurrence Records, and iNaturalist observations (after verifying identifications) in the area of interest have also been incorporated to examine current and historic presence of species. Site visits were conducted in the spring and summer 2021-2023 to groundtruth the GIS mapping and assemble species checklists for individual sites in every major concentration of “prairies” and outcrops; due to limited access in much of the region, vegetation plots were not feasible. A total of 1393 recently extant prairie patches were mapped spanning 2191.57 hectares. 744 vascular plant species associated with these communities are documented here. I propose 17 distinct community types, including grasslands, woodlands, and forests correlated with different substrates, disturbance regimes, and topographic positions, currently lumped under a few broad concepts in the U.S. National Vegetation Classification. Long-term distributional trends are difficult to analyze due to inconsistencies in historic records, but it is clear that the extent, quality, and diversity of all of these communities has declined drastically in both recent decades and post-settlement at a regional scale, and a significant portion (perhaps even a majority) of the historic acreage of the “Jackson Prairie” represents likely extinct community types associated with more mesic, loamy, and acidic soils than current remnants. More detailed and quantitative investigation is needed to refine and validate these community concepts; this survey and assessment should provide a basis for further research and inform conservation planning in the region.
Additional file 6 of Developing a Bayesian hierarchical model for a prospective individual patient data meta-analysis with continuous monitoring
Creators: name: Wu, Danni
name: Goldfeld, Keith S.
name: Petkova, Eva
Publisher: name: figshare
Year: 2024
Subjects: Mathematical Sciences not elsewhere classified
Cancer
Science Policy
Computational Biology

description: Additional file 6. a R code for simulation [33, 44, 45]. b Stan code for the final version of the co model.
Can't Break Kayfabe
Creators: name: Holt, Thaddeus Theodore
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2023
Subjects: Dance
Wrestling
Professional Wrestling
Theater
Dramatic Art
Sports

description: Professional wrestling is an art form standing on the intersection of sports and theater, politics and pop culture, and reality and fiction. It depends on a fictionalization of reality known as "kayfabe", through which values are performed for the approval or rejection of an audience. Understanding the history of professional wrestling, and how it first became fictionalized, allows for a greater understanding of the performances which are carried out by politicians and celebrities in our own world, as we all attempt to navigate the performative realities and unbreakable kayfabe of real life. Throughout eleven sections, this paper seeks to define kayfabe and its importance to the nature of performance in professional wrestling and beyond. It does so first by examining the lingo and structure of the professional wrestling art form. It then goes on to examine the history of professional wrestling, from the craft's infancy to the modern day, and how it has remained relevant across an evolving media landscape. Finally, it seeks to tackle the questions of the definition of professional wrestling as theater, sport, or dance, and how the performance of wrestling has affected our popular culture and politics in the United States of America and throughout the world. Written with both the veteran fan and complete wrestling new-comer in mind, this paper is an attempt at answering the question of what makes the drama of professional wrestling so uniquely engaging.
Fibrinogenγ 390-396A protects against high fat diet-induced obesity and SLD by altering glycine metabolism
Creators: name: Riya Nitesh Shah
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2023

description: Obesity is a major health concern in the US and a significant risk factor for numerous diseases, including fatty liver disease, type 2 diabetes and cardiovascular diseases. Our lab has previously shown that deposition of extravascular fibrin(ogen), the main structural constituent of the blood clot, is increased in white adipose tissue and liver of obese individuals. These fibrin(ogen) deposits colocalize with pro-inflammatory macrophages, and mice expressing fibrinogen with a mutation in the binding site for macrophages (Fibγ390-396A mice) were protected against diet-induced obesity (DIO) and associated pathologies. NMR-based metabolomic analyses identified elevated levels of glycine in high-fat diet (HFD)-fed Fibγ390-396A mice compared to HFD-fed FibγWT mice, consistent with reports indicating that obesity is associated with reduced circulating glycine levels. The goal of the study was to elucidate the mechanism by which fibrinogen-macrophage interaction regulates glycine metabolism and subsequent obesity development. Here, we document altered hepatic expression of glycine-serine metabolic pathways in HFD-fed Fibγ390-396A mice compared to HFD-fed FibγWT mice. Interestingly, while glycine supplementation was protective against DIO in HFD-fed FibγWT mice, glycine supplementation did not provide further protection in HFD-fed Fibγ390-396A mice. Similarly, glycine supplementation suppressed fatty liver disease progression in FibγWT mice similarly to Fibγ390-396A mice. Our data suggests that the Fibγ390-396A mice are protected against DIO and NAFLD through modification of the glycine-serine metabolism pathway and alterations in coagulation factors, highlighting a novel interaction between coagulation factors and metabolic pathways.
Bidirectional Tests on Two Shaft-Grouted Barrette Piles in Mekong Delta, Vietnam
Creators: name: H. M. Nguyen, B. H. Fellenius
Publisher: name: Southeast Asian Geotechnical Society
Year: 2016
Subjects: Bidirectional tests
Barrette piles
Shaft grouting
Analysis of strain-gage records

description: The Geotechnical Engineering Journal of the SEAGS & AGSSEA, 47, 1, pp 15-25
Homologous Recombination during CRISPR-Induced DNA Double-Strand Breaks
Creators: name: Reese Leanna Perini
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2023

description: Homologous recombination repair (HR) is a vital DNA repair mechanism essential for organismal growth and survival. Misregulation of HR can lead to loss of heterozygosity (LOH) in tumor suppressor genes, potentially facilitating the development of genetic diseases such as cancer. LOH occurs when a functional tumor suppressor allele on one homologous chromosome is replaced by a mutant allele from the other homologous chromosome through HR. This results in homology for a nonfunctional tumor suppressor. Homologous loss of tumor suppressor function promotes cancerous transformation. Most DNA double-strand breaks are resolved into noncrossover (NCO) genetic products by HR. Double-Holliday junction (dHJ) resolution, a type of HR with an intermediate DNA configuration called a dHJ, leads to rarer crossover (CO) products. COs formed during mitosis can lead to dangerous exchanges between homologous chromosomes and LOH. In order to test proposed models and decipher precise mechanisms of dHJ resolution, we reconstructed CRISPR/Cas9 DNA repair products in Drosophila melanogaster using PCR and Oxford Nanopore sequencing. Our findings support previously proposed models of dHJ cleavage. We also found products that are not readily explained by current models. These unique products characterize exceptions to the proposed models of dHJ cleavage, helping us to better understand this recombination mechanism. Results provide insights into how COs form in general, and how HR works in a CRISPR/Cas9 context, which can lead to more efficient and successful gene-editing strategies in the future.
Additional file 1 of Tea consumption and risk of lung diseases: a two‑sample Mendelian randomization study
Creators: name: Chen, Linjie
name: Deng, Yaru
name: Wang, Tiexu
name: Lin, Xinyu
name: Zheng, Lukun
name: Chen, Xiaohong
name: Chen, Tongsheng
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Biotechnology
subject: Environmental Sciences not elsewhere classified
subject: Sociology
subject: FOS: Sociology
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Science Policy
subject: Infectious Diseases
subject: FOS: Health sciences

description: Supplementary Material 1: Table S1: Detailed information of the genome-wide association study (GWAS) used in this study. Table S2: Mendelian randomization analysis of tea intake. Table S3: Mendelian randomization analysis of tea intake and IPF. Table S4: Mendelian randomization analysis of tea intake and lung cancer. Table S5: Mendelian randomization analysis of tea intake and COPD. Table S6: Mendelian randomization analysis of tea intake and acute bronchitis. Table S7: Mendelian randomization analysis of tea intake and tuberculosis. Table S8: Mendelian randomization analysis of tea intake and pneumonia
Droughts and Despair: An Innovative Tool for Policy-Makers to Assess Climate Change's Economic Impact on "Diseases of Despair" in Farming Communities
Creators: name: Arsene, C
Publisher: name: Zenodo
Year: 2025

description: The technical documentation contains the information necessary to use the on-line resources available  https://github.com/acc25uom/climate_change_study  and to generate the results. 

These developments were obtained for the research project funded by Wellcome Trust UK and called "Droughts and Despair: An Innovative Tool for Policy-Makers to Assess Climate Change's Economic Impact on “Diseases of Despair” in Farming Communities" (grant number 228264/Z/23/Z).
Preconception Care of Men
Creators: name: Mamahit, Citra Gabriella
name: Sumiya, Yuyu
name: Augustin, Gessica
Publisher: name: figshare
Year: 2024
Subjects: classificationCode: 420603
subject: Health promotion

description: Preconception care (PCC) aims to enhance the health of individuals before pregnancy, but research has primarily focused on women. Recently, there has been increasing interest in men's preconception health due to its impact on fertility and overall well-being. Despite this, there is a lack of comprehensive research in this area.
Soil Characterization and Land Subsidence Prediction for the First MRT Line in HCM City
Creators: name: Pham Huy Giao And Ta Thi Thoang
Publisher: name: Southeast Asian Geotechnical Society
Year: 2016
Subjects: HCM City
Subsoil database
Land subsidence
MRT line
Groundwater extraction
Soft clay
Sai Gon- Dong Nai delta

description: The Geotechnical Engineering Journal of the SEAGS & AGSSEA, 47, 1, pp 26-31
Additional file 1 of Attention-deficit/hyperactivity disorder symptoms and subsequent cardiometabolic disorders in adults: investigating underlying mechanisms using a longitudinal twin study
Creators: name: Dobrosavljevic, Maja
name: Kuja-Halkola, Ralf
name: Li, Lin
name: Chang, Zheng
name: Larsson, Henrik
name: Du Rietz, Ebba
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Physiology
subject: Biotechnology
subject: Sociology
subject: FOS: Sociology
subject: Biological Sciences not elsewhere classified
subject: Science Policy
subject: Mental Health

description: Additional file 1: Table S1. Diagnostic codes for the cardiovascular and metabolic disorders included in the study, according to the International Classification of Diseases 10th revisions (ICD-10) and medication prescriptions coded according to the Anatomical Therapeutic Classification (ATC). Table S2. Incidence of clinical diagnoses/dispensed medication prescriptions for cardiovascular and metabolic disorders as distinct categories, and associations with ADHD score, expressed as hazard ratios (HR) with 95% confidence intervals (CI), adjusted for birth year and sex. Table S3. Associations of scores on the inattention (IA) and hyperactivity/impulsivity (HI) subscales, with cardiovascular (CVD) and metabolic disorders, presented as hazard ratios (HR) with 95% confidence intervals (CI). Table S4. Frequencies for clinically diagnosed individuals and individuals with research diagnosis of ADHD. Table S5. Associations of ADHD score with cardiovascular disorders (CVDs) and metabolic disorders, as hazard ratios with 95% confidence intervals for men. Table S6. Associations of ADHD score with cardiovascular disorders (CVDs) and metabolic disorders, as hazard ratios with 95% confidence intervals for women. Table S7. Associations of ADHD score with cardiovascular disorders (CVDs) and metabolic disorders, as hazard ratios with 95% confidence intervals (CI) for monozygotic (MZ) twin pairs, and dizygotic (DZ) twin pairs with the same-sex female/male twin pairs. Supplementary methods. Description of DSM-IV criteria for psychiatric comorbidities. Fig. S1-S6. Natural cubic spline function to model non-linear associations between ADHD symptoms and cardiometabolic disorders. Table S8. Associations between quartiles, the 90th and 99th percentile of ADHD scores and the risk of cardiometabolic outcomes.
Soft Ground Improvement by Deep Cement-Mixing Technique in Southern Vietnam
Creators: name: Shiwakoti, Dinesh Raj
Publisher: name: Southeast Asian Geotechnical Society
Year: 2016
Subjects: Deep cement-mixing technique
Dry mixing method
Soft ground
Uniaxial compressive strength
Stiffness
Permeability

description: The Geotechnical Engineering Journal of the SEAGS & AGSSEA, 47, 1, pp 32-38
Body Positive? An Ethnographic Study of Teen Engagement with Body Positive Music and Marginalized Identity
Creators: name: Flanagan, Anne
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2023

description: One of the most powerful social contexts that shapes adolescent identity development is the engagement with popular music. This thesis presents an ethnographic analysis of how youth at Girls Rock North Carolina (GRNC) summer camp interacted with three contemporary pop songs containing “body-positive” themes. The analysis is framed within the concept of body positivity, herein defined as accepting and embracing the entire diverse range of bodies found in society. Camper focus groups and my observations of the youths’ engagement with music reveal three main themes pertaining to body-positive music and the larger role of music in social justice work.  First, in the context of GRNC camp, music primarily acts as a vessel for social justice values; second, body image is deeply entwined with gender identity and expression; and, third, even in spaces that promote unapologetic self-expression, the broader social pressures that the youth encounter compound to prescribe limited assumptions about what musicians look like, which affects youths’ self-perception. 
These three findings have broader implications for how so-called body-positive music operates and youth audiences respond. Analysis reveals that “body-positive” music often reinforces cisgender, heterosexual, and white racial norms, which inherently conflicts with the inclusive definition of body positivity. Many of these songs convey to audiences that they are not normal and that is okay, rather than affirming diverse identities as inherently normal. Ultimately, GRNC youth primarily engaged with music that depicted everyday experiences of those with shared marginalized identities, suggesting that a broader range of representative music is important in youth development.
Modelo criptográfico baseado em autômatos celulares heterogêneos para imagens digitais
Creators: name: Araújo Lima, Danielli
name: Silva, Eduardo Cassiano da
Publisher: name: figshare
Year: 2022
Subjects: Applied Computer Science

description: O principal objetivo da criptografia de dados é possibilitar que duas entidades se comuniquem ao longo de um canal inseguro, de tal maneira que nenhum oponente consiga decifrar a mensagem que é enviada. Além disso, deve-se garantir de que as informações transmitidas não serão copiadas, modificadas ou falsificadas. Muitos algoritmos de criptografia clássica e moderna já foram investigados para garantir a troca segura de informações. No entanto, se o dado transmitido for uma imagem, os algoritmos clássicos de criptografia não beneficiam o arranjo nem a quantidade de informações redundantes que são características estruturais das mesmas. Assim, uma nova abordagem para este tema são os autômatos celulares (ACs), que estão sendo estudados pela simplicidade de implementação e também por sua capacidade de processar dados em paralelo. Neste trabalho um novo modelo criptográfico baseado autômatos celulares heterogêneos será elaborado. Esse novo método utiliza no processo de cifragem o cálculo de pré-imagens, que corresponde à evolução do AC para trás. Um conjunto de regras caóticas serão responsáveis pela cifragem efetiva da mensagem e uma borda será adicionada para garantir a existência de qualquer pré-imagem. Esse modelo será fortemente baseado no algoritmo de Gutowitz, um modelo criptográfico precursor que utiliza ACs unidimensionais. Inicialmente, um estudo dos modelos precursores baseados na técnica do cálculo de pré-imagens foi realizado e o modelo de Gutowitz foi implementado na linguagem de programação C. A definição do novo modelo de criptografia, bem como os experimentos realizados para sua validação e refinamento, serão realizados nas próximas etapas. Com os testes que serão realizados posteriormente, espera-se obter um modelo que seja uma opção interessante para a cifragem de grandes quantidades dados, especialmente as imagens digitais.
An Unconventional Deformation of the Spin-1/2 Fermi Gas
Creators: name: Palanivelrajan, Vimal
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2023

description: Particles in nature are either bosons or fermions. Bosons are characterized by the fact that many of them can occupy the same quantum state, whereas fermions are limited by the Pauli principle, whereby only one particle can occupy a given a given state at any given time. In this work, we explore a possible generalization of quantum statistics that goes beyond this paradigm, with a focus on nonrelativistic interacting systems in a conformally invariant limit.

Interacting bosonic and fermionic systems can be expressed in terms of their noninteracting versions (in an external field) by using a Hubbard-Stratonovich transformation. When treating interactions in that fashion, the quantum statistics enters through the form of the noninteracting partition function Z0 in an external field. The logarithm of Z0 for bosons and fermions are distinguished mathematically, by the former being given by an infinite series (in the possible occupations of single-particle states), while the latter is a truncated sum due to the Pauli principle. By truncating the bosonic series in different ways, one obtains an extension of quantum statistics in an unconventional direction that interpolates between fermions and bosons, where only up to K particles may occupy a single-particle state, with K varying between 1 and infinity.

Here we explore such a deformation of quantum statistics in a particular case, which amounts to twisting the chemical potential of the Fermi gas in the complex plane. In particular, in the conformally invariant case of the unitary limit of spin-1/2 fermions, we obtain a class of equally conformally invariant theories of particles obeying alternate statistics. We provide a first description of the thermodynamics of such a system and compare it with known results for conventional spin-1/2 fermions. Notably this alternative statistics does not display a sign problem, which paves the way for future quantum Monte Carlo calculations of this system.
Communicating Food Waste Reduction: A Case Study of ReFED’S Strategic Communications
Creators: name: Geier, Charlotte
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2023

description: One of the greatest ironies of our nation’s eating habits is that despite there being 34 million hungry people in the U.S., research shows 38% of our food, or 91 million tons, goes to waste along the supply chain each year. Food waste reduction requires engagement from all stakeholders involved in the food waste sphere. This paper performs a case study analysis of a successful food waste reduction nonprofit, ReFED, to understand how its strategy is distinctive and effective in communicating food waste reduction. The paper concludes with a set of key takeaways and recommendations moving forward for ReFED’s strategic communications and other food waste reduction nonprofits.
Additional file 5 of SIMplyBee: an R package to simulate honeybee populations and breeding programs
Creators: name: Obšteter, Jana
name: Strachan, Laura K.
name: Bubnič, Jernej
name: Prešern, Janez
name: Gorjanc, Gregor
Publisher: name: figshare
Year: 2024
Subjects: subject: Genetics
subject: FOS: Biological sciences

description: Additional file 5. Genomics vignette. This vignette demonstrates how to obtain genomic informationof simulated honeybees. It also demonstrates, how to compute honeybeegenomic relationship matrices in SIMplyBee [62–67]. This vignette can also be found on  https://cran.r-project.org/package=SIMplyBee and  http://www.SIMplyBee.info .
Over Consolidation Feature of Clayey Soils in Southern Vietnam According to Piezocone
Creators: name: Bui Truong Son, Le Hong Quang
Publisher: name: Southeast Asian Geotechnical Society
Year: 2016
Subjects: Soft soil
Over-consolidation ratio
Piezocone
Relationship

description: The Geotechnical Engineering Journal of the SEAGS & AGSSEA, 47, 1, pp 39-44
Characterization of Potent Fully Modified siRNAs That Preferentially Silence KRAS G13D Transcripts in Cancer Cells
Creators: name: Jayaprakash, Vandanaa
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2023

description: Mutations in KRAS are well-established drivers of cancer that cause the overactivation of the MAPK pathway, leading to uncontrolled cellular division. Although KRAS G12C-specific small molecule inhibitors have shown success, this isoform only represents 11% of all KRAS mutations. Existing treatment approaches for all other KRAS mutations, including G12D, G12C, G12V and G13D, are indirect, non-mutant selective and primarily focused on blocking the downstream effectors of KRAS. The inhibition of such downstream signaling leads to a reduction in activity of these specific targets across the entire system, leading to potential cellular toxicity. RNAi is a unique genetic tool that has the potential to selectively bind mutant KRAS mRNA while sparing its wild-type counterpart, thus mitigating systemic cellular toxicity. The objective of this project is to identify the most potent sequence and chemical modifications for KRAS G13D mutant specific siRNAs. The KRAS G13D mutation is a top source of lung and pancreatic cancers and currently has limited therapeutic options. We started with 15 uniquely designed siRNAs based on a parental siRNA and identified four lead G13D siRNAs for further evaluation. We determined that siRNAs EFTX 7 and EFTX 10 preferentially reduced the mutant KRAS mRNA versus the wild-type and had the highest knockdown efficiency. At the translational level, western blot analysis demonstrated KRAS knockdown for both candidates. To potentially enhance specificity to the mutant, we integrated modifications from both EFTX 7 and EFTX 10 onto the parental siRNA construct, creating a combined EFTX 7+10 oligonucleotide. Preliminary mRNA data shows preferential reduction in mutant KRAS compared to the wild-type by EFTX 7+10. Analysis at the protein level for the combined construct and in vivo studies testing whether KRAS silencing using siRNAs produces off-target effects warrants further
investigation. By elucidating the most potent modifications, novel siRNA oligonucleotides that preferentially inhibit the KRAS G13D mutant can be developed, revolutionizing cancer therapy.
Supplemental Material, statement_letter - The Neurocognitive and Behavioral Profiles of 3 Brothers With Becker Muscular Dystrophy
Creators: name: Danique M. J. Hellebrekers
name: Vles, Johan S. H.
name: Klinkenberg, Sylvia
name: Hendriksen, Jos G. M.
Publisher: name: SAGE Journals
Year: 2020
Subjects: subject: 110904 Neurology and Neuromuscular Diseases
subject: FOS: Clinical medicine
subject: 111403 Paediatrics
subject: Neuroscience

description: Supplemental Material, statement_letter for The Neurocognitive and Behavioral Profiles of 3 Brothers With Becker Muscular Dystrophy by Danique M. J. Hellebrekers, Johan S. H. Vles, Sylvia Klinkenberg and Jos G. M. Hendriksen in Child Neurology Open
Ground Improvement Using Soil-Cement Method: A Case Study with Laboratory Testing and In-Situ Verification for a Highway Project in Southern Vietnam
Creators: name: Vu, Phan To Anh
Publisher: name: Southeast Asian Geotechnical Society
Year: 2016
Subjects: Soil-cement method
Sable Soil cement
Tower slag cement
UCS

description: The Geotechnical Engineering Journal of the SEAGS & AGSSEA, 47, 1, pp 45-49
Report on multi-sectoral management. Version 1.0
Creators: name: BELLOULID, Oussama
Publisher: name: Zenodo
Year: 2025

description: This milestone report for Task 2.3, "Pathways Exploration and Optimization Towards Multi-Sectoral Water Management," outlines progress in identifying and refining strategies for sustainable water storage and distribution across the project's demonstration sites. Building on a thorough characterization of each site’s environmental, hydrological, and socio-economic context, Task 2.3 explores innovative pathways that leverage natural and artificial infrastructure to balance water needs across sectors. These efforts are informed by extensive literature reviews, lessons from similar sites, and participatory approaches, such as Living Labs (LLs) and stakeholder-driven innovation ecosystems.
description: This project is part of the PRIMA Programme supported by the European Union’s Horizon 2020 Research and Innovation Programme under Grant Agreement No 2222.
Additional file 8 of Developing a Bayesian hierarchical model for a prospective individual patient data meta-analysis with continuous monitoring
Creators: name: Wu, Danni
name: Goldfeld, Keith S.
name: Petkova, Eva
Publisher: name: figshare
Year: 2024
Subjects: Mathematical Sciences not elsewhere classified
Cancer
Science Policy
Computational Biology

description: Additional file 8. The effect of sample sizes on the model’s performance.
Exploring the involvement of BPIFA1 protein in asthma pathophysiology and the mechanism of action of albuterol
Creators: name: Knee, Alexa
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2023

description: Asthma is a prevalent respiratory disease for which albuterol is a very common treatment. It is widely thought that albuterol binds to beta-2 adrenergic receptors on airway smooth muscle (ASM), causing ASM relaxation and allowing for bronchodilation and relief of asthma symptoms. Bactericidal/permeability-increasing protein fold-containing family member A1 (BPIFA1) is an epithelium-derived smooth muscle relaxing factor that also causes ASM relaxation. The goal of this study was to determine if asthmatic cell cultures have lower levels of BPIFA1 and if albuterol causes ASM relaxation indirectly by stimulating BPIFA1 release from epithelial cells. Expression and concentration of BPIFA1 were measured in asthmatic and non-asthmatic human bronchial epithelial (HBE) cells via qPCR and western blot after treatment with albuterol. Additionally, ASM cells were treated with beta blocker before treatment with albuterol and/or treatment with the proteins secreted from epithelial cells to determine if BPIFA1 provides protection against ASM constriction. Expression and secretion of BPIFA1 were found to be lower in asthmatics. Additionally, treatment with albuterol led to significantly increased RNA expression of BPIFA1 and BPIFA1 secretion in asthmatic HBE cells but not in non-diseased control HBE cells. This finding reflects that albuterol acts on epithelial beta-2 receptors and augments BPIFA1 release from airway epithelial cells, elucidating a mechanism of action of albuterol that causes ASM relaxation through driving the release of proteins from HBE cells.
Additional file 2 of SIMplyBee: an R package to simulate honeybee populations and breeding programs
Creators: name: Obšteter, Jana
name: Strachan, Laura K.
name: Bubnič, Jernej
name: Prešern, Janez
name: Gorjanc, Gregor
Publisher: name: figshare
Year: 2024
Subjects: subject: Genetics
subject: FOS: Biological sciences

description: Additional file 2. Multiple colonies vignette. This vignette introduces working with multiple colonies bydemonstrating how to create and work with MultiColony objects inSIMplyBee. This vignette can also be found on https://cran.r-project.org/package=SIMplyBee and  http://www.SIMplyBee.info .
SARS-CoV-2 Vaccine Effectiveness for Patients Undergoing Immunosuppressive Treatment
Creators: name: Coleman, Grayson
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2023

description: The urgency spurred by the SARS-CoV-2 pandemic has propelled an essential shift towards investigating the efficacy of vaccinations in protecting immunosuppressed patients. We honed our investigations on the efficacy of the SARS-CoV-2 vaccine suspecting these patients would have lower protection against the virus than healthy controls due to a reduced and/or inhibited adaptive immune repertoire. Peripheral T cells were analyzed to determine proliferation fitness as well as measure the activation of Th1, Th17, and Tfh subtypes through cytokine analysis. Patients demonstrated varying degrees of protection against the novel coronavirus compared to healthy controls. Further inspection also demonstrated an influx of IFNy production in both healthy controls and patients following booster vaccination (the third vaccine received). Cytokine analysis showed no significant differences in cytokine production in these subtypes between healthy controls and immunosuppressed patients which would correlate to a difference in immune capability. These findings demonstrated that patients and healthy controls produced similar responses to the RBD peptide leading us to believe patient T cells may not be greatly impacted by immunosuppressive therapies. Percent inhibition of neutralizing antibodies to the SARS-CoV-2 antigen were then subsequently calculated from peripheral B cells to determine their immune fitness. The resulting data demonstrated that immunosuppressed patient B cells elicited either an inadequate, moderate, or adequate antibody response. The three clusters identified, we believe, are governed by an unidentified indicator not directly related to primary demographic information, though, tethered to the timing of patient immunosuppressive treatment administration. These results underscore the importance of addressing vaccination strategies for immunosuppressed patients, highlighting the need for tailored approaches to ensure their protection and greater public safety.
Additional file 2 of Convergent evolution of the sensory pits in and within flatworms
Creators: name: Gąsiorowski, Ludwik
name: Dittmann, Isabel Lucia
name: Brand, Jeremias N.
name: Ruhwedel, Torben
name: Möbius, Wiebke
name: Egger, Bernhard
name: Rink, Jochen C.
Publisher: name: figshare
Year: 2024
Subjects: subject: Genetics
subject: FOS: Biological sciences

description: Additional file 2: Fig. S1. Phylogenetic analysis of PRD-class homeobox transcription factors. Fig. S2. Phylogenetic analysis of nuclear receptor subfamily 2. Fig. S3. Phylogenetic analysis of Pax sequences. Fig. S4. Phylogenetic analysis of Dach sequences. Fig. S5. Phylogenetic analysis of Emx sequences. Fig. S6. Molecular phylogeny of flatworms inferred with a maximum likelihood approach from the concatenated 18S, 28S, ITS-5.8S, and COI datasets.
CED-12/ELMO prevents fragmentation of a non-protrusively migrating leader cell in C. elegans
Creators: name: Li, Karen
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2023

description: The distal tip cell (DTC) is the leader cell that drives the formation of the gonad in hermaphrodite C. elegans. Previous genetic analyses have shown that the C. elegans ortholog of the small GTPase Rac1, CED-10, and its activating GEF complex comprised of CED-5, CED-2, and CED-12, are required for proper distal tip cell migration and resulting gonad morphology. Rac1 is classically required for the formation of protrusions at the leading edge of a migrating cell (Delorme et al., 2007). However, the DTC migrates entirely non-protrusively. It was not yet known how a complex of genes that are typically required for protrusive cell migration impacts the migration of a non-protrusive cell so robustly. We hypothesized that knocking down the ced-12 gene function affects distal tip cell migration by directly impacting morphology and cytoskeletal regulation of the distal tip cell (the germline stem-cell niche in the C. elegans gonad) during the migration process will affect proper DTC migration and development and result in migrational and morphological defects. Our research employed the use of genetic knockdowns (RNAi), CRISPR, cloning, and live confocal imaging and analysis. We discovered that knocking down the ced-12 gene function resulted in the bifurcation of the gonad and fragmentation of the distal tip cell. Our cell of interest is the stem cell niche for a population of germline stem cells that go on to form sperm or eggs in C. elegans germline, so understanding how this stem cell niche functions sheds light on fundamental principles of stem cell maintenance, cell fate regulation, and developmental plasticity that have broader relevance to stem cell biology and potential applications in regenerative medicine and disease treatment.
VisualizingRegionalMobility_FINAL_SupplementaryMaterial – Supplemental material for Visualizing the Regionalized Structure of Mobility between Countries Worldwide
Creators: name: Deutschmann, Emanuel
Publisher: name: SAGE Journals
Year: 2020
Subjects: subject: Sociology
subject: FOS: Sociology

description: Supplemental material, VisualizingRegionalMobility_FINAL_SupplementaryMaterial for Visualizing the Regionalized Structure of Mobility between Countries Worldwide by Emanuel Deutschmann in Socius
Discrete Modelling of Excavation in Fractured Rock by NSCD Method
Creators: name: Hang, Tran Thi Thu
Publisher: name: Southeast Asian Geotechnical Society
Year: 2016
Subjects: Fractured rock
NSCD
LMGC90
Multi-phase-excavation
Simulation

description: The Geotechnical Engineering Journal of the SEAGS & AGSSEA, 47, 1, pp 62-68
Luminary: An Open-Source Initiative for Crowdsourced Accessible Campus Navigation
Creators: name: Mendoza, Christine
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2023

description: People with disabilities face numerous obstacles to accessing campus activities in the form of physical
barriers (ex. stairs, broken elevators, steep inclines, sidewalk blockages) and knowledge barriers (e.g. lack of information on accessibility equipment status, confusing signage for accessible routes). Luminary aims to address knowledge barriers around campus route-finding by crowdsourcing information on obstacles
to suggest routes customized to the needs of the specific user.
PCR-based survey of methane-cycling archaea in methane-soaked subsurface sediments of Guaymas Basin, Gulf of California
Creators: name: Hinkle, John
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2023

description: The Guaymas Basin in the Gulf of California is characterized by active seafloor spreading, rapid deposition of organic-rich sediments, steep geothermal gradients, and abundant methane of mixed thermogenic and microbial origin. Subsurface sediment samples from eight drilling sites with distinct geochemical and thermal profiles were selected for DNA extraction and PCR amplicifation to explore the diversity of methane-cycling archaea in the Guaymas Basin subsurface. We performed PCR amplifications with general (mcrIRD), and ANME-1 specific primers that target the alpha (α) subunit of methyl coenzyme M reductase (mcrA). Diverse ANME-1 lineages associated with anaerobic methane oxidation were detected in seven out of the eight drilling sites, preferentially around the methane-sulfate interface, and in several cases showed preferences for specific sampling sites. Phylogenetically, most ANME-1 sequences from the Guaymas Basin subsurface were related to marine mud volcanoes, seep sites, and the shallow marine subsurface. The most frequently recovered methanogenic phylotypes were closely affiliated with the hyperthermophilic Methanocaldococca-ceae, and found at the hydrothermally influenced Ringvent site. The coolest drilling site, in the northern axial trough of Guaymas Basin, yielded the greatest diversity of methanogen lineages. Our survey indicates potential for extensive microbial methane cycling within subsurface sediments of Guaymas Basin.
Enabling new strategies for switchable and tunable nickel catalysis
Creators: name: Huebsch, Elsa
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2023

description: Catalysis is a wide-ranging and fundamental tool for the synthesis of compounds
essential to modern life. It is also ubiquitous in biological systems through proteins called enzymes, and synthetic chemists have
long pursued the kind of control and flexibility achieved by these structures. Switchable catalysts are structures which allow the reactivity of transition metal complexes to be modulated via external stimuli. Recent work has expanded upon pre-existing switchable catalysis strategies through hydrogen bonding networks and novel ligand frameworks for stabilizing different formal oxidation states of nickel.
Supplemental Material, JCPT_Contributor_Agreement_-_Signed - Comparing Diuresis Patterns in Hospitalized Patients With Heart Failure With Reduced Versus Preserved Ejection Fraction: A Retrospective Analysis
Creators: name: Broscious, Rachael
name: Kukin, Alina
name: Noel, Zachary R.
name: Devabhakthuni, Sandeep
name: Hyunuk Seung
name: Ramani, Gautam V.
name: Reed, Brent N.
Publisher: name: SAGE Journals
Year: 2020
Subjects: subject: Cardiology
subject: 110306 Endocrinology
subject: FOS: Clinical medicine
subject: 111599 Pharmacology and Pharmaceutical Sciences not elsewhere classified
subject: 110203 Respiratory Diseases
subject: 110323 Surgery

description: Supplemental Material, JCPT_Contributor_Agreement_-_Signed for Comparing Diuresis Patterns in Hospitalized Patients With Heart Failure With Reduced Versus Preserved Ejection Fraction: A Retrospective Analysis by Rachael Broscious, Alina Kukin, Zachary R. Noel, Sandeep Devabhakthuni, Hyunuk Seung, Gautam V. Ramani and Brent N. Reed in Journal of Cardiovascular Pharmacology and Therapeutics
A Method for Estimating Pile Group Settlement Considering Distribution of Pile Shaft Friction (SDF) Application for Pile Groups in Vietnam
Creators: name: Duong Diep Thuy, Pham Quang Hung
Publisher: name: Southeast Asian Geotechnical Society
Year: 2016
Subjects: Pile group
Settlement
Friction along pile
Vietnam

description: The Geotechnical Engineering Journal of the SEAGS & AGSSEA, 47, 1, pp 69-78
sj-pdf-1-jir-10.1177_0022185620956685 - Supplemental material for Cross-spousal influences on mature-aged Australians’ transitions in and out of employment 2001–2017
Creators: name: Craig, Lyn
name: Churchill, Brendan
Publisher: name: SAGE Journals
Year: 2020
Subjects: subject: 150310 Organisation and Management Theory
subject: FOS: Economics and business

description: Supplemental material, sj-pdf-1-jir-10.1177_0022185620956685 for Cross-spousal influences on mature-aged Australians’ transitions in and out of employment 2001–2017 by Lyn Craig and Brendan Churchill in Journal of Industrial Relations
A novel role for Histone H4 Lysine 20 in Hox gene regulation in Drosophila
Creators: name: Mukherjee, Aadit
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2023

description: Polycomb Group (PcG) proteins are conserved chromatin-modifying factors involved in silencing gene expression in Drosophila. Notable among the genes that are regulated by this pathway are the developmentally important Hox genes. Different histone residues have been shown to be involved in the Polycomb silencing, including Histone H3 Lysine-27 and -36. One residue that does not have a well-documented role in this pathway is Histone H4 Lysine-20 (H4K20). We showed that when H4K20 was mutated in Drosophila, adult flies exhibited phenotypes that were typical of dysregulation of Hox genes by the PcG proteins. We performed a viability assay and quantified PcG homeotic transformations to confirm the implication that H4K20 participates in Hox gene repression. We also carried out immunofluorescence of larval brains to determine the involvement of H4K20 in specific Hox genes, and western blots to identify potential mechanisms for its involvement. Our findings suggest that H4K20 is indeed involved in Polycomb regulation, but the mechanism through which it acts is yet to be determined.
Calibration System and Magnetic Shielding Development for NuDot
Creators: name: Vickers, Sarah
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2023

description:  The success of future kiloton-scale liquid scintillator neutrino experiments is dependent on novel advancements in background reduction and isotope loading techniques. Otherwise irreducible backgrounds such as boron solar neutrino scattering can be identified by their event topology using Cherenkov light signals. NuDot is a half-ton prototype aiming to demonstrate this technique, using precision timing to separate Cherenkov and scintillation signals in 1 to 2 MeV beta particles. The goal of the current surface data-taking campaign is to demonstrate Cherenkov light-based directional reconstruction with a collimated beta calibration source. Presented here is a collection of improvements to the NuDot experiment, contributing to the efficiency and accuracy of data collection during this phase. Included is the optimized mathematical models, design, and implementation plan for the geo-magnetic field shielding, which will reduce the magnetic field in the active volume of the detector to less than 10% of the present geo-magnetic field, allowing a PMT efficiency of 99%. The second element is a series of hardware and electronics alterations, namely for the calibration system, essential for testing directional reconstruction.
Supplementary_Materials – Supplemental material for Association Between Parity and the Age at Menopause and Menopausal Syndrome in Northwest China
Creators: name: Sun, Xiaoyan
name: Zhang, Rui
name: Wang, Lirong
name: Shen, Xiping
name: Lu, Yongbin
name: An, Junxia
name: Wang, Liyan
name: Wang, Yiqing
name: Luo, Xiaorong
name: Zhu, Haiying
name: Zhang, Xuehong
Publisher: name: SAGE Journals
Year: 2020
Subjects: Public Health and Health Services not elsewhere classified

description: Supplemental material, Supplementary_Materials for Association Between Parity and the Age at Menopause and Menopausal Syndrome in Northwest China by Xiaoyan Sun, Rui Zhang, Lirong Wang, Xiping Shen, Yongbin Lu, Junxia An, Liyan Wang, Yiqing Wang, Xiaorong Luo, Haiying Zhu and Xuehong Zhang in Asia Pacific Journal of Public Health
U.S. Imperialism and the Panamá Canal Zone: Culture, Music, and Panamanian Nationalism
Creators: name: Héctor Aizpurúa
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2023

description: My honors thesis documents the history of the depopulation of the Panama Canal Zone between 1904-1916. I aim to rehumanize the inhabitants of the isthmus through their music and history. The inhabitants were described as less than human by Americans working and traveling through Panamá during the construction of the Canal.
Additional file 1 of SIMplyBee: an R package to simulate honeybee populations and breeding programs
Creators: name: Obšteter, Jana
name: Strachan, Laura K.
name: Bubnič, Jernej
name: Prešern, Janez
name: Gorjanc, Gregor
Publisher: name: figshare
Year: 2024
Subjects: subject: Genetics
subject: FOS: Biological sciences

description: Additional file 1. Honey biology vignette. This vignette introduces SIMplyBee package by describing anddemonstrating how SIMplyBee implements honeybee biology. Specifically, itdescribes how to initiate simulation with founder genomes and simulationparameters, how to create and build-up a colony, the colony structure, andcomplementary sex determininglocus [20, 21] This vignette can also be found on https://cran.r-project.org/package=SIMplyBee and  http://www.SIMplyBee.info .
Regularization Strategies on Estimators: Estimating the Effect of Dongguan’s 2014 Anti-Vice Campaign
Creators: name: NIu,
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2023

description: The Chinese Ministry of Public Security's Anti-Vice Campaign in Dongguan City in 2014 was a crackdown on prostitution (illegal in China), which took place mostly in local hotels. This paper builds on Doudchenko and Imbens (2016)'s work by examining how policy effect estimation methods with different regularization strategies yield different estimates for the Average Treatment Effect on Dongguan’s hospitality industry (ATT). The set of estimation methods discussed in the paper includes Difference-in-Difference, Constrained Regression, Synthetic Control Method, Synthetic Difference-in-Difference, and Best Subset Selection. Validity checks of these estimation methods (e.g., Parallel Trend Assumption for Difference-in-Difference) are also discussed. The major finding of this paper is that the estimated policy effect on the outcome variable Number of Overnight Tourists (see Section 4.1 for outcome variable description) is a 44.3% to 54.2% decrease, concluded by the set of estimation methods; the regularization strategies in the Synthetic Control Method enable dimensionality reduction, which is empirically useful. Therefore, researchers are recommended to run SCM first to benefit from the dimensionality reduction property.