cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Insights into the thermal stabilization and conformational transitions of DNA by hyperthermophile protein Sso7d: molecular dynamics simulations and MM-PBSA analysis
Creators: name: Chen, Lin
name: Zheng, Qing-Chuan
name: Yu, Li-ying
name: Chu, Wen-Ting
name: Zhang, Ji-Long
name: Xue, Qiao
name: Zhang, Hong-Xing
name: Sun, Chia-Chung
Publisher: name: Taylor & Francis
Year: 2012
Subjects: Molecular Biology

description: In the assembly of DNA-protein complex, the DNA kinking plays an important role in nucleoprotein structures and gene regulation. Molecular dynamics (MD) simulations were performed on specific protein-DNA complexes in this study to investigate the stability and structural transitions of DNA depending on temperature. Furthermore, we introduced the molecular mechanics/Poisson–Boltzmann surface area (MM-PBSA) approach to analyze the interactions between DNA and protein in hyperthermophile. Focused on two specific Sso7d-DNA complexes (PDB codes: 1BNZ and 1BF4), we performed MD simulations at four temperatures (300, 360, 420, and 480 K) and MM-PBSA at 300 and 360 K to illustrate detailed information on the changes of DNA. Our results show that Sso7d stabilizes DNA duplex over a certain temperature range and DNA molecules undergo B-like to A-like form transitions in the binary complex with the temperature increasing, which are consistent with the experimental data. Our work will contribute to a better understanding of protein-DNA interaction.
Evaluation of a Non-Invasive Method for Isolation and Analysis of Uterine Immune Cells.
Creators: name: Samsami, Daniel
Publisher: name: figshare
Year: 2017
Subjects: subject: Immunology
subject: FOS: Clinical medicine
subject: Medicine

description: During pregnancy, the maternal immune system is one of the major players responsible for a successful implantation of the embryo and development of the placenta. Ineffective implantation often leads to pregnancy complications such as low birth weight and preeclampsia. Uterine natural killer cells (uNK) reside in the uterine mucosa and interact with extravillous trophoblast cells, in order to establish an efficient blood flow to the growing embryo and placenta. Although studies have identified uNK-specific receptor and ligand combinations, which increase the risk for pregnancy complications, the interaction between uNK cells and trophoblast cells has not been studied in detail on a cellular level. This is mostly due to problems associated with obtaining clinically relevant material. Previous studies have shown that menstrual blood contains uNK cells, but their viability after freezing/thawing has not been investigated. The possibility to freeze and thaw cells is very useful since it allows long-term storage as well as group analysis. In the present study we have therefore investigated the possibility of using thawed menstrual blood cells for flow cytometric analysis. Our study shows that an average of 92,16% uNK cells from thawed menstrual blood cells are negatively stained with a viability marker and an apoptosis marker. These results indicate that freezing menstrual blood cells for future analysis is possible, and that menstrual blood can be used as a non-invasive source of uNK cells.
Characterization of induced Staphylococcus aureus bacteriophage SAP-26 and its anti-biofilm activity with rifampicin
Creators: name: Yong Seol, Sung
name: Kim, Jungmin
name: Rahman, Marzia
name: Kim, Shukho
name: Kim, Sung Min
Publisher: name: Taylor & Francis
Year: 2011
Subjects: Marine Biology

description: Lytic bacteriophages (phages) have been investigated as treatments for bacterial infectious diseases. An induced phage, SAP-26, was isolated from a clinical isolate of Staphylococcus aureus. It belongs to the family Siphoviridae and its genome consists of double-stranded 41,207 bp DNA coding for 63 open reading frames. The phage SAP-26 showed a wide spectrum of lytic activity against both methicillin-resistant S. aureus and methicillin-susceptible S.aureus. Furthermore, combined treatment with a phage and antimicrobial agents showed a strong biofilm removal effect which induced structural changes in the biofilm matrix and a substantial decrease in the number of bacteria. Such a broad host range in S. aureus and biofilm removal activity of the phage SAP-26 suggests the possibility of its use as a therapeutic phage in combination with appropriate antimicrobial agent(s). Among the three antimicrobial agents combined with phage, the combination of rifampicin showed the best biofilm removal effect. To the authors' knowledge, this study showed for the first time that S. aureus biofilm could be efficiently eradicated with the mixture of phage and an antimicrobial agent, especially rifampicin.
Value added product development for oyster farmers' group in Kung Krabaen Bay Royal Development Study Center, Chanthaburi
Creators: name: Duanrung Benjamas
Publisher: name: Nakhon Pathom Rajabhat University
Year: 2020
Subjects: Oyster, oyster product development, oyster in Kung Krabaen, oyster product

description: Interdisciplinary Research Review, 15, 1, 54
Impact of cranberry juice on initial adhesion of the EPS producing bacterium Burkholderia cepacia
Creators: name: Yang, Xuejiao
name: Liu, Yang
name: Teng, Fucheng
name: Zeng, Hongbo
Publisher: name: Taylor & Francis
Year: 2012
Subjects: Marine Biology

description: The impact of cranberry juice was investigated with respect to the initial adhesion of three isogenic strains of the bacterium Burkholderia cepacia with different extracellular polymeric substance (EPS) producing capacities, viz. a wild-type cepacian EPS producer PC184 and its mutant strains PC184rml with reduced EPS production and PC184bceK with a deficiency in EPS production. Adhesion experiments conducted in a parallel-plate flow chamber demonstrated that, in the absence of cranberry juice, strain PC184 had a significantly higher adhesive capacity compared to the mutant strains. In the presence of cranberry juice, the adhesive capacity of the EPS-producing strain PC184 was largely reduced, while cranberry juice had little impact on the adhesion behavior of either mutant strain. Thermodynamic modeling supported the results from adhesion experiments. Surface force apparatus (SFA) and scanning electron microscope (SEM) studies demonstrated a strong association between cranberry juice components and bacterial EPS. It was concluded that cranberry juice components could impact bacterial initial adhesion by adhering to the EPS and impairing the adhesive capacity of the cells, which provides an insight into the development of novel treatment strategies to block the biofilm formation associated with bacterial infection.
Additional file 1 of Genome-wide analysis and expression profile of the bZIP gene family in poplar
Creators: name: Zhao, Kai
name: Chen, Song
name: Yao, Wenjing
name: Cheng, Zihan
name: Zhou, Boru
name: Jiang, Tingbo
Publisher: name: figshare
Year: 2021
Subjects: subject: Genetics
subject: FOS: Biological sciences

description: Additional file 1: Supplemental Table 1. Annotations of bZIP protein sequence motifs.
Pseudolymphoma in a cat on phenobarbital treatment
Creators: name: Lieser, Julia
Publisher: name: figshare
Year: 2017
Subjects: subject: 60804 Animal Immunology
subject: FOS: Biological sciences

description: Figure 1 - Photomicrograph of a fine needle aspirate of an enlarged popliteal lymph node in a cat treated with phenobarbital.Table 1 - CBC results
Table 2 - Biochemistry results
Additional file 2 of Genome-wide analysis and expression profile of the bZIP gene family in poplar
Creators: name: Zhao, Kai
name: Chen, Song
name: Yao, Wenjing
name: Cheng, Zihan
name: Zhou, Boru
name: Jiang, Tingbo
Publisher: name: figshare
Year: 2021
Subjects: subject: Genetics
subject: FOS: Biological sciences

description: Additional file 2: Supplemental Table 2. Segmentally duplicated bZIP gene pairs in poplar.
The DMRT gene family in amphioxus
Creators: name: Wang, Fei
name: Yu, Yang
name: Li, Hongyan
name: Ji, Dongrui
Publisher: name: Taylor & Francis
Year: 2012
Subjects: Molecular Biology

description: Doublesex and Mab-3-related transcription factor (DMRT) gene family is widely known for its involvement in sex determination and/or differentiation among different phyla. In this study, we identify eight DMRT genes in the cephalochordate amphioxus, a protochordate holding a key phylogenetic position. The eight DMRTs can be divided into two groups based on the conserved domain: BfDM044, BfDM045, BfDM55.1, BfDM115.1, and BfDM17.1 belong to the first group which have both DM and DMA domains, while BfDM246.1, BfDM084, and BfDM175 belong to the second group which have only DM domain. Most of the first group members have same genomic structure except BfDM17.1, while no regular pattern exists in the second group. Phylogenetic analysis of the DM domain sequences shows that DMRT genes in vertebrates form seven different independent clusters, and some even contain genes from invertebrates with high bootstrap. Notably, the first group members of amphioxus cluster with vertebrate DMRTs; while the second group members cluster into a single branch, which diverge from the vertebrate classes. The results suggest that several DMRT genes in vertebrates may evolve from homologous genes in invertebrates. As in nematode, drosophila, fish, and vertebrates, DMRT genes cluster is also found in amphioxus, which may be the result of gene duplication. Interspecific differences in the amphioxus DMRTs and sea squirt DMRTs may suggest post-speciation duplication of some DMRT genes.
Design of a Learner-Directed E-Learning Model
Creators: name: Lee, Stella
Publisher: name: University of Hertfordshire
Year: 2014
Subjects: e-learning
instructional design
learning design
student modelling
Kolb
Experiential Learning Theory
Self-Regulated Learning Theory
learning experience
learning computer programming

description: How can one create online educational material that support and motivate students in guiding their own learning and make meaningful instructional decisions? One of the main focuses on designing e-learning is about creating an environment where learners can actively assume control and take responsibility for their own learning with little or no guidance from the tutors. This research aims to discover a new way to design learning that would cater to individual choices and preferences. The idea goes beyond learner-centred design; it is about learner control and direction. As an option, learners should be able to choose to be in the driver’s seat, to direct their own learning journey. As a starting point, this research explores the use of two educational theories - Experiential Learning Theory (ELT) and Self-Regulated Learning (SRL) theory as the underpinning instructional design for a Learner-Directed Model to support students’ online learning in both domain knowledge and meta knowledge in the subject of computer programming. One unit material from an online Introduction to Java Programming course has been redesigned based on the proposed Learner-Directed Model for the experimental design study. The study involved a total of 35 participants divided randomly into one Experimental Group and one Control Group. They were assigned to either a Learner-Directed Model (Experimental Group) or a linear model (Control Group). Pre/post tests, survey, follow-up interview as well as log file analysis were instruments used for assessing students’ domain knowledge, meta knowledge and their attitudes for their overall learning experience. Learning experience is further broken down into perceived ease of use and user satisfaction; system usability; learner experience; and perceived controllability. The results of the study have revealed that there is statistically significant difference between the survey results for the Experimental Group and the Control Group. The Experimental Group reported a higher level of overall learning experience and better attitudes in general. However, there was no statistically significant difference existing between the two groups on the domain and meta level knowledge improvement. Based on these results, I have proposed further research directions and put forward a number of recommendations and suggestions on learner-directed e-learning design.
Investigating the Properties of Brown Dwarfs Using Intermediate-Resolution Spectroscopy
Creators: name: Canty, James Ignatius
Publisher: name: University of Hertfordshire
Year: 2015
Subjects: stars: formation
pre-main sequence
low-mass
brown dwarfs
atmospheres
luminosity function
mass function

description: This thesis is an investigation into some properties of brown dwarfs using medium-resolution spectroscopy. In the first part of the thesis, I address the issue of parameter degeneracy in brown dwarfs. In the course of my analysis, I derive a gravity-sensitive spectral index which can be used, statistically at least, to differentiate populations of young objects from field dwarfs. The index is also capable of finding the difference between a population of ~1 Myr objects and a population of ~10 Myr objects and may be used to separate low-mass members from foreground and background objects in young clusters and associations. The second part of my thesis is an investigation into the major opacity sources in the atmospheres of late T dwarfs. I look particularly at CH4 and NH3 absorption features in the near-infrared spectra of these objects. In my analysis, I identify new absorption features produced by these molecules. I also correct features which had previously been wrongly identified. This has been made possible by the use of high quality data, together with a new CH4 synthetic line list, which is more complete at these temperatures than any previously available list
COST Action TU1207 Next Generation Design Guidelines for Composites in Construction: State-of-the-Art Report
Creators: name: Guadagnini, Maurizio
Publisher: name: The University of Sheffield
Year: 2017
Subjects: subject: 90599 Civil Engineering not elsewhere classified
subject: FOS: Civil engineering
subject: 91202 Composite and Hybrid Materials
subject: FOS: Materials engineering
subject: 90502 Construction Engineering
subject: 90503 Construction Materials

description: This State-of-the-Art report included 76 contributions on key areas of development, from material development to testing, design and application.The publication discusses:
- Material Development and Characterisation
- New Reinforced Concrete (RC) Structures
- Strengthening Applications
- Whole-life-costing and life cycle assessments
- Advanced Composites in the COST Countries: Research and Development
Simultaneous determination of chloramphenicol, florfenicol and florfenicol amine in ham sausage with a hybrid chemiluminescent immunoassay
Creators: name: Jiang, Haiyang
name: Wang, Xia
name: Wang, Zhanhui
name: Wu, Xiaoping
name: Shen, Jianzhong
name: Tao, Xiaoqi
name: Yu, Xuezhi
name: Zhu, Jinghui
name: Niu, Lanlan
Publisher: name: Taylor & Francis
Year: 2013
Subjects: subject: Microbiology
subject: FOS: Biological sciences

description: A novel chemiluminescent immunoassay utilising two types of primary antibodies (murine monoclonal antibody and rabbit polyclonal antibody) and two types of horseradish peroxidase–labelled secondary antibodies was established for simultaneously detecting multiple amphenicol residues in ham sausage. After combining the extract procedure of the target amphenicol into one simplified method, this hybrid chemiluminescent immunoassay could screen chloramphenicol (CAP), florfenicol (FF) and its metabolite florfenicol amine (FFA) at the same time by adding the corresponding secondary antibody. Ham sausage samples were analysed by using this hybrid immunoassay, with LODs of CAP being 0.01 μg kg−1, of FF being 2.8 μg kg−1 and of FFA being 3.0 μg kg−1. The applicability of the proposed method has been validated by determining CAP, FF and FFA in ham sausage samples with satisfactory results. Good recoveries and high correlation with traditional enzyme-linked immunosorbent assay and LC-MS/MS results illustrated that the developed hybrid chemiluminescent immunoassay could screen high-throughput ultra-trace amphenicol residues effectively at one time.
Additional file 2: of Age at menarche and prevention of hypertension through lifestyle in young Chinese adult women: result from project ELEFANT
Creators: name: Guo, Liqiong
name: Peng, Cheng
name: Xu, Hui
name: Wilson, Ander
name: Li, Peng-hui
name: Wang, Hao
name: Liu, Hongbin
name: Shen, Lilin
name: Chen, Xi
name: Qi, Xiuying
name: Tang, Nai-jun
name: Barrow, Timothy
name: Byun, Hyang-Min
Publisher: name: figshare
Year: 2018
Subjects: subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Sociology
subject: FOS: Sociology
subject: Mathematical Sciences not elsewhere classified
subject: Computational Biology

description: Odds ratios (95% CIs) for hypertension related to age at menarche by BMI. (DOCX 27 kb)
МЕТОДИКА ПРЕПОДАВАНИЯ ЛЕКСИКИ И ФРАЗЕОЛОГИИ
Creators: name: Гиёсова, Василя
name: Хомиджонова, Оминабону
Publisher: name: Zenodo
Year: 2025

description: Статья посвящена методике изучения лексики и фразеологии в преподавании русского языка. Анализируются цели и задачи обучения этим разделам, принципы и методы преподавания, а также этапы формирования лексических и фразеологических умений у школьников. Особое внимание уделено дидактическим материалам и системам упражнений, способствующим эффективному усвоению лексических единиц и фразеологических выражений. Работа ориентирована на педагогов, методистов и студентов филологических факультетов, заинтересованных в совершенствовании методики преподавания русского языка.
Art of the ancestors: Spatial and temporal patterning in the rock art of Nawarla Gabarnmang, a major Jawoyn cultural site on the Arnhem Land plateau
Creators: name: Gunn, Robert George
Publisher: name: Monash University
Year: 2019
Subjects: Archaeology

description: The thesis develops and applies a new systematic approach to the archaeological recording of rock art. Using the Arnhem Land rock art site of Nawarla Gabarnmang, which has over 1400 images and a 50,000 year cultural history, the study combined DStretch enhancement, Harris Matrices and the Morellian Method to successfully identify and document the individual images to establish the spatial and temporal structure of the site’s art history.
Efficacy of Fungicides on Coexisting Leptophaeria spp. Causing Phoma Stem Canker on Winter Oilseed Rape
Creators: name: Sewell, Thomas
Publisher: name: UH Research Archive
Year: 2017
Subjects: fungicides
phoma stem canker
oilseed rape
Leptosphaeria
fungicide resistance

description: Phoma stem canker is a disease of oilseed rape (Brassica napus) caused by closely related plant pathogens Leptosphaeria maculans and L. biglobosa. It is an economically important disease, causing annual yield losses of approximately £770 million worldwide. When colonising oilseed rape, L. maculans and L. biglobosa exist in close proximity on the leaf, competing for resources as they move through the main leaf vein and into the stem. Fungicides are commonly used to decrease severity of phoma stem canker on oilseed rape. However, the efficacy and longevity of active chemicals is under threat from evolution of resistance in pathogen populations and government legalisation. Moreover, it has been suggested that both L. maculans and L. biglobosa differ in their sensitivity to azoles, and important class of fungicides that are used to control the disease through the inhibition of lanosterol 14-α demethylase (erg11, CYP51). This project aims to further understand the role that fungicides have in controlling phoma stem canker by investigating their efficacy against L. maculans and L. biglobosa in crops, in vitro and in planta. In field experiments, established in Cambridgeshire across four cropping seasons, the fungicide mixture penthiopyrad (SDHI) plus picoxystrobin (QoI) was as effective at controlling phoma leaf spotting and phoma stem canker in winter oilseed rape as prothioconazole (DMI), suggesting that both fungicides could be used to reduce phoma stem canker symptoms. The two pathogens differed in their growth rates in vitro, with L. biglobosa growing faster than L. maculans when untreated or treated with lower fungicide concentrations. Fungicide sensitivity assays suggest that L. maculans and L. biglobosa are both sensitive to DMI, SDHI and QoI fungicides and that differences between the species are minor. Prothioconazole and penthiopyrad + picoxystrobin had a similar efficacy on oilseed rape cotyledons colonised with either L. maculans or L. biglobosa. There was no difference between species on prothioconazole treated plants, although there was a difference between L. maculans and L. biglobosa when treated with 20 μg/ml penthiopyrad + picoxystrobin. Heterologous yeast expression of LmCYP51B and LbCYP51B with fungicide sensitivity testing of the yeast transformants suggests that LmCYP51B and LbCYP51B are similarly sensitive to azole fungicides flusilazole, prothioconazole-desthio and tebuconazole. These findings are supported by homology protein modelling, which predicts that LmCYP51B and LbCYP51B are structurally very similar, specifically at the azole-binding site. In conclusion, fungicides are still an effective control method for reducing phoma stem canker symptoms caused by Leptosphaeria species in the UK, and a useful tool to in the sustainable production of oilseed rape.
Dynamics of biofilm formation under different nutrient levels and the effect on biofouling of a reverse osmosis membrane system
Creators: name: Chen, Xi
name: McDougald, Diane
name: Kjelleberg, Staffan
name: Rice, Scott A.
name: Raditya Suwarno, Stanislaus
name: Haur Chong, Tzyy
name: Cohen, Yehuda
name: Fane, Anthony G.
Publisher: name: Taylor & Francis
Year: 2013
Subjects: Marine Biology

description: Pseudomonas aeruginosa PAO1 wild type and a mucoid derivative (FRD1) which over produces alginate were used to foul reverse osmosis (RO) membranes. When operated at a constant flux, biofilm formation on the RO membrane resulted in a slow rise in transmembrane pressure (TMP) of 22% for the initial four days of operation, followed by a sharp increase of 159% over the following two days. The initial slow increase in TMP was probably due to the formation of a biofilm on the membrane surface, which then accelerated the rate of biofouling through the effect of concentration polarization. At later stages of operation, most of the bacterial biomass consisted of dead cells. The amount of extracellular polymeric substances appeared to correlate positively with the number of dead cells. The results indicate that prolonging the initial stage of slow TMP increase and avoiding the latter stage of accelerated TMP increase would provide a sustainable operation of the RO system. These results suggest that nutrient limitation could reduce biofilm accumulation and delay the increase in TMP.
Additional file 3 of Genome-wide analysis and expression profile of the bZIP gene family in poplar
Creators: name: Zhao, Kai
name: Chen, Song
name: Yao, Wenjing
name: Cheng, Zihan
name: Zhou, Boru
name: Jiang, Tingbo
Publisher: name: figshare
Year: 2021
Subjects: subject: Genetics
subject: FOS: Biological sciences

description: Additional file 3: Supplemental Table 3. List of syntenic gene pairs.
Video of Procedure
Creators: name: Izzy Watters
name: Chesser, Mary
name: Wallace, Demetria
name: Pennington, Skyler
name: Hager, Mindy
name: Wagge, Jordan
name: Hamilton, David M
name: Harte, Ryan
name: Staves, Taylor
name: Gardner, Sydney
name: Nninah Freelon
name: Blackburn, Taylor
name: Vaughn, Aaron
name: Williams, Shaun
name: Tumelo M Musamali
name: Echols-Spratling, Anaya
name: Urquidi, Stephanie
Publisher: name: OSF Registries
Year: 2020

description: This component contains a video of the survey.
Preorientation of protein and RNA just before contacting
Creators: name: Xiao, Yi
name: Guo, Dachuan
name: Liu, Shiyong
name: Huang, Yangyu
Publisher: name: Taylor & Francis
Year: 2013
Subjects: Molecular Biology

description: Protein and RNA molecules interact and form complexes in many biological processes. However, it is still unclear how they can find the correct docking direction before forming complex. In this paper, we study preorientation of RNA and protein separated at a distance of 5–7 Å just before they form contacts and interact with each other only through pure electrostatic interaction when neglecting the influence of other molecules and complicated environment. Since geometric complementary has no meaning at such a distance, this is not a docking problem and so the conventional docking methods, like FTDock, are inapplicable. However, like the usual docking problem, we need to sample all the positions and orientations of RNA surrounding the protein to find the lowest energy orientations between RNA and protein. Therefore, we propose a long-range electrostatic docking-like method using Fast Fourier Transform-based sampling, LEDock, to study this problem. Our results show that the electrostatically induced orientations between RNA and protein at a distance of 5–7 Å are very different from the random ones and are much closer to those in their native complexes. Meanwhile, electrostatic funnels are found around the RNA-binding sites of the proteins in 62 out of 78 bound protein–RNA complexes. We also tried to use LEDock to find RNA-binding residues and it seems to perform slightly better than BindN Server for 23 unbound protein–RNA complexes.
Additional file 4 of Genome-wide analysis and expression profile of the bZIP gene family in poplar
Creators: name: Zhao, Kai
name: Chen, Song
name: Yao, Wenjing
name: Cheng, Zihan
name: Zhou, Boru
name: Jiang, Tingbo
Publisher: name: figshare
Year: 2021
Subjects: subject: Genetics
subject: FOS: Biological sciences

description: Additional file 4: Supplemental Table 4. Annotations of DEGs.
Synthesis and hemocompatibity evaluation of segmented polyurethane end-capped with both a fluorine tail and phosphatidylcholine polar headgroups
Creators: name: Zhang, Xiaoqing
name: Fu, Qiang
name: Tan, Dongsheng
name: Li, Jiehua
name: Tan, Hong
Publisher: name: Taylor & Francis
Year: 2011
Subjects: Marine Biology

description: To improve the hemocompatibility of polyurethanes, an amine monomer containing a long fluorine tail and phosphatidylcholine polar headgroups, 2-amino-3-oxo-3-(2-(2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-pentadecafluorooctan amido) ethyl amino) propyl phosphorylcholine (FASPC) was firstly synthesized and characterized. Then four kinds of fluorinated phosphatidylcholine end-capped polyurethanes with different chemical structures were prepared. The surface properties of these prepared polyurethanes were characterized using X-ray photoelectron spectroscopic analysis (XPS) and water contact angle measurements. The results indicated that the phosphatidylcholine (PC) polar headgroups along with the fluorine tail could be easily enriched on the top surfaces, and the PC groups could be highly oriented on the outmost surface when the polymer film was in contact with water for only 30 s at room temperature. The evaluation of hemocompatibity was carried out via fibrinogen adsorption and platelet adhesion. Fibrinogen adsorption (37°C for 90 min) decreased by 98% to 87% compared to that on ordinary polyurethane surfaces, and almost no platelet adhesion and activation was observed at 37°C for 2 h.
GENTLE/A - Adaptive Robotic Assistance for Upper-Limb Rehabilitation
Creators: name: Gudipati, Radhika
Publisher: name: University of Hertfordshire
Year: 2014
Subjects: Upper-limb rehabilitation
Stroke
Human Robot Interaction
Adaptable system
Lead-lag model
Haptics for rehabilitation
Robot-assisted training
Assistive technologies
Biomechanics of the upper-limb
Embedded-Virtual environments

description: Advanced devices that can assist the therapists to offer rehabilitation are in high demand with the growing rehabilitation needs. The primary requirement from such rehabilitative devices is to reduce the therapist monitoring time. If the training device can autonomously adapt to the performance of the user, it can make the rehabilitation partly self-manageable. Therefore the main goal of our research is to investigate how to make a rehabilitation system more adaptable. The strategy we followed to augment the adaptability of the GENTLE/A robotic system was to (i) identify the parameters that inform about the contribution of the user/robot during a human-robot interaction session and (ii) use these parameters as performance indicators to adapt the system. Three main studies were conducted with healthy participants during the course of this PhD. The first study identified that the difference between the position coordinates recorded by the robot and the reference trajectory position coordinates indicated the leading/lagging status of the user with respect to the robot. Using the leadlag model we proposed two strategies to enhance the adaptability of the system. The first adaptability strategy tuned the performance time to suit the user’s requirements (second study). The second adaptability strategy tuned the task difficulty level based on the user’s leading or lagging status (third study). In summary the research undertaken during this PhD successfully enhanced the adaptability of the GENTLE/A system. The adaptability strategies evaluated were designed to suit various stages of recovery. Apart from potential use for remote assessment of patients, the work presented in this thesis is applicable in many areas of human-robot interaction research where a robot and human are involved in physical interaction.
Additional file 5 of Genome-wide analysis and expression profile of the bZIP gene family in poplar
Creators: name: Zhao, Kai
name: Chen, Song
name: Yao, Wenjing
name: Cheng, Zihan
name: Zhou, Boru
name: Jiang, Tingbo
Publisher: name: figshare
Year: 2021
Subjects: subject: Genetics
subject: FOS: Biological sciences

description: Additional file 5: Supplemental Table 5. Primer sequences.
Analysis and Optimisation of a Novel Wind Turbine
Creators: name: Zhang, Xu
Publisher: name: University of Hertfordshire
Year: 2014
Subjects: Urban Wind Turbine
Wind Energy
omputational fluid dynamic (CFD)
Wind Tunnel
Wind Turbine aerodynamics

description: The technologies of urban wind turbines have been rapidly developed in recent years, but urban wind turbines have not found a wide application due to the limitations of their designs. The power output of urban wind turbine is significantly affected by urban terrain, which can cause low speed flow with frequent change of its direction. Thus, there is a need for a new wind turbine to meet the requirements of an urban wind turbine. In this study, a novel wind turbine for urban areas was designed and developed. The investigations of the novel urban wind turbine were carried out by using computational fluid dynamic (CFD) simulations and wind tunnel tests. The results from the investigation have shown that the novel wind turbine has a great potential to harvest wind energy in urban areas. A detailed study of effects of each parameter on wind energy concentration of the novel wind turbine was carried out with CFD simulations. According to the simulation results, the shroud structure of the novel wind turbine was modified and the dimensions of the final structure were identified. It was determined that the capability of wind energy concentration of the novel wind turbine shroud has been significantly improved through the structure optimisations. Furthermore, guide vane and impulse turbine were implemented in the novel wind turbine. The flow characteristics through the guide vane was studied and discussed. It was found that the wind flow characteristics can be properly modified by implementing guide vane and the structure of impulse turbine was suitable to be implemented in the novel wind turbine due to the flow characteristic through the guide vane.
The prediction of protein structural class using averaged chemical shifts
Creators: name: Lin, Hao
name: Ding, Chen
name: Yang, Ping
name: Ding, Hui
name: Chen, Wei
name: Song, Qiang
name: Deng, Ke-Jun
Publisher: name: Taylor & Francis
Year: 2012
Subjects: Molecular Biology

description: Knowledge of protein structural class can provide important information about its folding patterns. Many approaches have been developed for the prediction of protein structural classes. However, the information used by these approaches is primarily based on amino acid sequences. In this study, a novel method is presented to predict protein structural classes by use of chemical shift (CS) information derived from nuclear magnetic resonance spectra. Firstly, 399 non-homologue (about 15% identity) proteins were constructed to investigate the distribution of averaged CS values of six nuclei (13CO, 13Cα, 13Cβ, 1HN, 1Hα and 15N) in three protein structural classes. Subsequently, support vector machine was proposed to predict three protein structural classes by using averaged CS information of six nuclei. Overall accuracy of jackknife cross-validation achieves 87.0%. Finally, the feature selection technique is applied to exclude redundant information and find out an optimized feature set. Results show that the overall accuracy increased to 88.0% by using the averaged CSs of 13CO, 1Hα and 15N. The proposed approach outperformed other state-of-the-art methods in terms of predictive accuracy in particular for low-similarity protein data. We expect that our proposed approach will be an excellent alternative to traditional methods for protein structural class prediction.
A New Rheological Polymer Based on Boron Siloxane Cross-Linked by Isocyanate Groups
Creators: name: Shmelin, George
Publisher: name: University of Hertfordshire
Year: 2012
Subjects: Polymer
rheology
silicone
siloxane
hetero-siloxane
polyurethane
polyurea-urethane
polyurethane-siloxane co-polymer
polymeric synthesis
DMA polymer analysis
FT-IR and HNMR/CNMR spectroscopy polymer analysis
IPN
scarcely cross-linked IPN
one side Grignard reaction of short αω-di halo olefins

description: The research described in this thesis originated from an idea to develop new body protection for the sport of fencing. The ultimate goal is to develop body armour which would be flexible, wearable, washable, light and breathable, offer protection from injuries and cover the entire body of a sportsman. A new material which exhibits shear thickening behaviour has been specially developed for this purpose in the process of this investigation. The material was designed and synthesised as a soft polymeric system which is flexible, chemically stable and able to increase the value of its modulus of elasticity upon impact at a high strain rate, while remaining in its soft gel-like elastomeric state when low strain rate deformation is applied. The polymeric system that has been developed is based on interpenetrating polymeric networks (IPN) of immiscible polyurethane/urea-ester/ether and poly(boron)n(dimethylsiloxane)m (where on average m ≈ 16 n). In addition, as the polydimethylsilane (PDMS) based polymeric system strongly tends to phase separation, the siloxane polymeric network was chemically cross-linked to the polyurethane polymeric network through polyurethane chemical cross-link-bridges. In order to introduce polyurethane cross-links to a siloxane-based polymeric network, some of the attached methyl groups in the PDMS polymeric backbone were substituted by ε-pentanol groups. The resulting polymeric system combines properties of an alternating copolymer with IPN. The actual substitution of the methyl groups of PDMS into alternating ε-pentanol groups was performed by Grignard reaction of trifunctional chlorosilane monomers, magnesium and 1,5-dibromopentane. Chemical analytical techniques like FT-IR, 13C NMR and 1H NMR spectroscopy were used to reveal the chemical structure of the synthesised polymeric network. The mechanical and dynamical properties of the obtained polymeric system were analysed by dynamic mechanical analysis (DMA). This part of the investigation indicated that the novel polymeric system exhibited shear thickening behaviour, but only at a narrow diapason of deformations (i.e., deformations between 2 to 3 % of the length of the sample). At this limited diapason of deformation an effective increase of the modulus of elasticity from 6 MPa (at lower frequencies, i.e., up to ≤6 Hz of the applied oscillating stress) to 65 MPa (at frequencies between 12.5 to 25 Hz) was obtained. However, no increase in the modulus of elasticity was recorded at deformations below 1.5 % or above 3.5 % of length of the sample at the same frequencies (0 to 25Hz) of the applied oscillating stress.
Using History in Public Policy Development
Creators: name: Green, Alix Rivka
Publisher: name: University of Hertfordshire
Year: 2013
Subjects: public history
historiography
policy
government
higher education
methodology
historical discipline

description: This thesis addresses two key problems: that historical practice in the academy is largely disengaged from politics as a domain of public purpose and that policymaking remains fixed on a very narrow (and quantitative) definition of evidence, although the “policy-relevant” disciplines have not proved able to solve long-standing policy issues. It inspects both phenomena with the aim of describing the space in which the two problems can be brought into a workable accommodation. The argument is made that public policy should be regarded as an important concern of academic history, and policymakers themselves as people with legitimate interests that historians should take seriously. Public history provides a helpful framework and set of concerns to work with in this respect. Given that the social and natural sciences have not been able to solve the pressing policy problems with which governments are faced, a certain obligation may be claimed for historians to reconsider their stance. The re-connection of history and policy – the nineteenth-century discipline clearly discerned a public-political purpose for history – requires attention to be given to articulating and demonstrating the distinctive cognitive tools of the historian and their distinctive value to the policymaking process. The thesis addresses two primary fields, whose interests and professional practices appear divergent such that both the principles and the terms of collaboration are difficult to imagine: academic history and government policymaking. The primary material on which the research draws is accordingly the products of these constituencies: works of historiography and policy documents of various kinds. Also of relevance are commentaries and analyses that address these domains, whether from other disciplines with an interest in political decision-making, from the media or from other organisations with a professional stake, such as think tanks. The originality of the research lies in conceiving of the question of the uses of history for public policy as one of integration of “supply” and “demand” perspectives. It seeks clarity on the distinctive value of historical skills and approaches, but not as an end in itself. Rather, the case is assembled for the affinities between history and policy as processes and hence that the two can be brought into a productive alignment. So, instead of history providing pre-packaged accounts for policy, it can be embedded as a way of thinking and reasoning in policy.
Prólogo al libro: La palabra electrónica
Creators: name: Rogel-Salazar, Rosario
Publisher: name: figshare
Year: 2017
Subjects: subject: Digital Humanities
subject: Language
subject: 200104 Media Studies
subject: FOS: Media and communications

description: Pólogo al libro: La palabra electrónica de Santiago Cortés Hernández, editado por el Fondo Editorial del Estado de México, Secretaria de Educación del Gobierno del Estado de México, 2014.
Este libro propone una discusión sobre las implicaciones conceptuales y cognitivas de los nuevos soportes para la escritura: ¿cómo ha cambiado la tecnología de la palabra escrita a partir de la disponibilidad de los medios electrónicos? ¿Se han transformado nuestros conceptos de lo escrito, del conocimiento y de la cultura a partir de la incorporación de la palabra electrónica a nuestra vida cotidiana? ¿Qué significa ser alfabetizado en la era digital? Sabemos que vivimos en una época de grandes transformaciones de lo escrito, pero, ¿cómo pasó todo esto?, ¿por qué cauces llegaron aquellos ríos de códigos binarios y dónde podemos ver sus efectos ahora que ha subido la marea electrónica?
MOESM1 of IL-2 augments the sorafenib-induced apoptosis in liver cancer by promoting mitochondrial fission and activating the JNK/TAZ pathway
Creators: name: Ding, Xiaoyan
name: Sun, Wei
name: Chen, Jinglong
Publisher: name: figshare
Year: 2018
Subjects: subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Pharmacology
subject: Chemical Sciences not elsewhere classified
subject: Immunology
subject: FOS: Clinical medicine
subject: Cancer

description: Additional file 1: Figure S1. The influence of IL-2 and sorafenib treatment on the viability of L02 normal liver cells. A. MTT assay was used to evaluate the cell viability. B. LDH release assay was performed to detect the cell death in response to Il-2 and sorafenib treatment.
Additional file 3: of Age at menarche and prevention of hypertension through lifestyle in young Chinese adult women: result from project ELEFANT
Creators: name: Guo, Liqiong
name: Peng, Cheng
name: Xu, Hui
name: Wilson, Ander
name: Li, Peng-hui
name: Wang, Hao
name: Liu, Hongbin
name: Shen, Lilin
name: Chen, Xi
name: Qi, Xiuying
name: Tang, Nai-jun
name: Barrow, Timothy
name: Byun, Hyang-Min
Publisher: name: figshare
Year: 2018
Subjects: subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Sociology
subject: FOS: Sociology
subject: Mathematical Sciences not elsewhere classified
subject: Computational Biology

description: Odds ratios (95% CIs) for hypertension related to age at menarche by psychological stress. (DOCX 26 kb)
Emission Line Stars In and Beyond the Perseus Arm
Creators: name: Raddi, Roberto
Publisher: name: University of Hertfordshire
Year: 2013
Subjects: Early-type stars
emission-line stars
Be stars
dust extinction
ISM structure
Galaxy structure

description: I present low-resolution (Dl 6 A° ) follow-up spectroscopy of 370 Ha emitters (12 . r . 17) identified with IPHAS, in a 100 deg2 wide section of the Galactic plane that is located between ` = (120 ; 140 ) and b = (􀀀1 ; +4 ). Classical Be stars are found to be the most numerous group of the observed targets ( 60%). Sixty-eight classical Be stars have also been observed at higher spectral resolution (Dl 2􀀀4 A° ) and S/N ratio, which allows spectral typing to an estimated precision of 1 sub-type. Colour excesses were measured via spectral energy distribution fitting of flux-calibrated data. I took care to remove the circumstellar contribution to the measured colour excess, using an established scaling to the Ha equivalent widths. In doing so, this method of correction was re-evaluated and modified to better suit the data at hand. Spectroscopic parallaxes were measured constraining the luminosity class via estimates of distances to main sequence A/F stars, which are found within a few arcminutes of each classical Be star on the sky. In order to probe the structure of the outer Galactic disc, I studied the spatial distribution of 63 out of 248 classical Be stars identified. Their cumulative distribution function with respect to the distance is statistically compatible both with a smooth exponential density profile and with a simple spiral arms representation. The distribution of reddenings of classical Be stars is compared with estimates of the total Galactic reddening along their sightlines. It is expected that the measured reddenings match the integrated Galactic values, for distant stars located outside the Galactic dust layer, or they are smaller than the asymptotic values if the stars are less distant. The outcome meets expectations, and lends support to the conclusion that the measured reddenings are determined to a precision of 10%. The sample of 248 objects doubles the number of known classical Be stars in this part of the Galactic plane. Unlike the pre-existing bright sample, the new objects are seen at large distances, between 2 – 8 kpc with typical E(B􀀀V) 0:9. Only four stars are members of known clusters. Ten classical Be stars are proposed to be well beyond the putative Outer Arm, at distances larger than 8 kpc. The large sample of stars, which has been identified here, is the result of a successful selection and analysis of classical Be stars that is offered for more exploitation in future. The proposition is that GAIA observations will use the present sample of classical Be stars as a new tracer of the Galactic disc.
The Biomechanics of the Dynamic Defence Mechanism
Creators: name: Gautrey, Charlotte
Publisher: name: University of Hertfordshire
Year: 2013
Subjects: Functional Ankle Instability
Postural Sway
Centre of Pressure Excursion
Muscle Latency
Reaction Time
Tilt Platform
Fatigue
Isokinetic Exercise
Peak Torque
Total Work

description: Context: It has been suggested that muscle fatigue can lead to injury, however, research investigating this phenomenon in functional ankle instability (FAI) subjects is lacking. Aim: The purpose of this thesis was to research postural sway and muscular latency in FAI subjects and healthy controls, both before and immediately after localised and globalised fatigue protocols. Subjects: All subjects used in this project were males, between the ages of 18 and 25 years, and participated in regular (>2 x week) aerobic exercise. Subjects were categorised into healthy subjects, or subjects with a history of FAI using the FAI questionnaire. Methods: Neuromuscular control was analysed in FAI subjects and healthy controls through measures of muscular latency and postural sway. These measures were repeated both before and immediately after localised and globalised fatigue protocols. Results: The induction of localised and globalised fatigue had no effect on muscle latency in the FAI or healthy subjects. However, postural sway was significantly increased in the FAI subjects, following localised and globalised fatigue, with globalised fatigue also significantly increasing postural sway in the healthy subjects. The globalised football-specific fatigue protocol caused the greatest deficits in the FAI subjects, but also the healthy controls. Conclusions: In terms of muscle latency individuals that participate in sports, as well as sports clinicians and coaches, should not be concerned about the theorised relationship between the onset of fatigue and an increased injury risk at the ankle. However, in terms of postural sway the globalised football-specific fatigue protocol caused the greatest deficits. This highlights that the fatigued individual may be at greater risk of musculoskeletal injury during prolonged exercise that involves multiple joints, such as a football match.
Revistas académicas de arte en bases de datos bibliográficas: disponibilidad en acceso abierto y en bibliotecas de tres instituciones mexicanas
Creators: name: Rogel-Salazar, Rosario
name: Martínez-Domínguez, Néstor Daniel
name: Vilchis, Ivonne Lujano
Publisher: name: figshare
Year: 2017
Subjects: subject: 80706 Librarianship
subject: FOS: Media and communications
subject: Library and Information Studies
subject: 80799 Library and Information Studies not elsewhere classified

description: We identify the presence of Art Scholary Journals in different bibliographic databases. In the case of journals recorded in the Arts & Humanities Citation Index –a&hci, Thomson Reuters isi– we identified their access type (open or by subscription), and its availability in three libraries of Mexican institutions linked to the arts. We found that there are no latinamerican Art Scholary Journals in the a&hci, its availability in open access is limited, as well as its availability in libraries analyzed, ranging from 20 to 6% in the cases analyzed.