Additional file 1 of Hsa_circ_0000098 is a novel therapeutic target that promotes hepatocellular carcinoma development and resistance to doxorubicin
Creators: name: Li, Yi
name: Wu, Anqi
name: Chen, Lin
name: Cai, Aiting
name: Hu, Yuhao
name: Zhou, Zhou
name: Qi, Qianyi
name: Wu, Yixuan
name: Xia, Donglin
name: Dong, Peixin
name: Ju, Shaoqing
name: Wang, Feng
Publisher: name: figshare
Year: 2022
Subjects: subject: Biochemistry
subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Pharmacology
subject: Chemical Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Hematology

description: Additional file 1: Figure S1. Cell apoptosis was detected with flow cytometry in HCC cells with circ_0000098 knockdown or overexpression. *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001. Figure S2. Silencing of circ_0000098 repressed HCC tumorigenesis in vivo. (A) Images of nude mice on days 7, 14, and 21 after subcutaneous inoculation with the indicated HCC cells. (B-D) The growth curve (B), tumor volume (C), and weight (D) of transplanted tumors were measured in the indicated groups. (E) Expression of hsa_circ_0000098 in the indicated groups. (F) HE-stained images and Ki67 expression images of tumors in the indicated groups. *P &lt; 0.05. Figure S3. The expression of miR-383 and MCUR1 in HCC cells. (A) The expression of miR-383 in TCGA liver cancer tissues and normal tissues (ENCORI database). (B) The relationship between the expression of miR-383 and the survival of HCC patients (KM plotter database). (C, D) Detection of miR-383 expression in BEL-7404 (C) and HCC-LM3 (D) cells transfected with control or miR-383 mimics (E) The expression of MCUR1 in TCGA liver cancer tissues and normal tissues (ENCORI database) (F) The relationship between the expression of MCUR1 and the survival of HCC patients (KM plotter database). (G, H) Detection of MCUR1 expression in BEL-7404 (G) and HCC-LM3 (H) cells transfected with control shRNA (sh-NC) or MCUR1 shRNA (sh-MCUR1). **P &lt; 0.01, ***P &lt; 0.001. Figure S4. KEGG pathway enrichment analysis (A) and GO biological processes annotation (B) of predicted miR-383 targets (ENCORI database). Figure S5. Effects of circ_0000098 and MCUR1 expression on HCC cell proliferation. (A-B) CCK-8 assays were performed in HCC-LM3 (A) and BEL-7404 (B) cells transfected as indicated. *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001. Figure S6. The expression of circ_0000098, miR-383, and MCUR1 in HCC tissues. (A-C) RT-qPCR assays were performed to detect circ_0000098 (A), miR-383 (B) and MCUR1 (C) expression in sh-1 or sh-NC-transfected groups (D) The relationship between the expression of ABCB1 (P-gp) and the survival of HCC patients (KM plotter database). *P &lt; 0.05, **P &lt; 0.01. Figure S7. Detection of the excitation process of DOX/sh-1@PLT by particle size analysis technology. Figure S8. DOX/sh-1@PLT inhibited HCC progression in vivo. (A) Detection of tumor size using computed tomography. (B) Detection of fluorescence intensity alterations in mouse HCC models. (C) Images of tumors from mouse HCC models. (D) Detection of DOX accumulation in the indicated tumors. NS: not significant. *P&lt;0.05.