title: ÐÐ¿ÑÐ¾Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ°Ñ Ð¿Ð»Ð°ÑÑÐ¾ÑÐ¼Ð° Ð´Ð»Ñ ÑÐ²ÐµÑÐ¾Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾Ð³Ð¾ ÑÐ¿ÑÐ°Ð²Ð»ÐµÐ½Ð¸Ñ Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐ¼Ð¸ Ð¿ÑÐ¾ÑÐµÑÑÐ°Ð¼Ð¸ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ ÑÐ°ÑÑÐ¸ÑÐµÐ»ÑÐ½ÑÑ ÑÐ¸ÑÐ¾Ñ ÑÐ¾Ð¼Ð¾Ð²
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Optogenetic platform for light-dependent control of intracellular processes based on plant phytochromes
Creators: name: ÐÐµÐ´ÐµÐ»ÑÐµÐ², ÐÐ²Ð³ÐµÐ½Ð¸Ð¹
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: Ð²Ð½ÑÑÑÐµÐ½Ð½Ðµ Ð½ÐµÑÐ¿Ð¾ÑÑÐ´Ð¾ÑÐµÐ½Ð½ÑÐµ Ð±ÐµÐ»ÐºÐ¸
Ð½ÐµÐ¼ÐµÐ¼Ð±ÑÐ°Ð½Ð½ÑÐµ Ð¾ÑÐ³Ð°Ð½ÐµÐ»Ð»Ñ
ÑÐ°Ð·Ð¾Ð²ÑÐµ Ð¿ÐµÑÐµÑ Ð¾Ð´Ñ ÑÐ¸Ð¿Ð° Â«Ð¶Ð¸Ð´ÐºÐ¾ÑÑÑ-Ð¶Ð¸Ð´ÐºÐ¾ÑÑÑÂ»
ÑÐ¸ÑÐ¾Ñ ÑÐ¾Ð¼Ñ ÑÐ°ÑÑÐµÐ½Ð¸Ð¹
ÑÐ¸ÑÐ¾Ñ ÑÐ¾Ð¼-Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²ÑÑÑÐ¸Ðµ Ð±ÐµÐ»ÐºÐ¸
ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ñ
Ð³ÐµÐ½Ð½Ð°Ñ Ð¸Ð½Ð¶ÐµÐ½ÐµÑÐ¸Ñ
Ñ Ð¸Ð¼ÐµÑÐ½ÑÐ¹ Ð±ÐµÐ»Ð¾Ðº
intrinsically disordered protein
membraneless organell
Â«liquid-liquidÂ» phase transition
plant phytochrome
phytochrome-interaction factor
fluorescence
genetic engineering
chimeric protein

description: Ð¤Ð¾ÑÐ¾ÑÐµÐ»ÑÑÐ° ÑÐ°ÑÑÐµÐ½Ð¸Ð¹ â ÑÑÐ¾ Ð½ÐµÐ¼ÐµÐ¼Ð±ÑÐ°Ð½Ð½ÑÐµ Ð¾ÑÐ³Ð°Ð½ÐµÐ»Ð»Ñ (ÐÐÐ), Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ðµ ÐºÐ¾ÑÐ¾ÑÑÑ Ð¸Ð½Ð¸ÑÐ¸Ð¸ÑÑÐµÑÑÑ Ð±ÐµÐ»Ð¾Ðº-Ð±ÐµÐ»ÐºÐ¾Ð²ÑÐ¼Ð¸ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸ÑÐ¼Ð¸ Ð¼ÐµÐ¶Ð´Ñ Ð°ÐºÑÐ¸Ð²-Ð½Ð¾Ð¹ ÑÐ¾ÑÐ¼Ð¾Ð¹ ÑÐ¸ÑÐ¾ÑÑÐ¾Ð¼Ð° B (phyB) Ð¸ ÑÐ¸ÑÐ¾ÑÑÐ¾Ð¼-Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²ÑÑÑÐ¸Ð¼Ð¸ ÑÐ°ÐºÑÐ¾ÑÐ°Ð¼Ð¸ (PIF). Ð­ÑÐ¸ Ð¾ÑÐ³Ð°Ð½ÐµÐ»Ð»Ñ Ð¿ÑÐ¸Ð½Ð¸Ð¼Ð°ÑÑ ÑÑÐ°ÑÑÐ¸Ðµ Ð² ÑÐ¾ÑÐ¾Ð¼Ð¾ÑÑÐ¾Ð³ÐµÐ½ÐµÐ·Ðµ ÑÐ°ÑÑÐµÐ½Ð¸Ð¹. Ð Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ Ð±ÑÐ» ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½ ÑÐ¸Ð¼ÐµÑÐ½ÑÐ¹ Ð±ÐµÐ»Ð¾Ðº PIF6-TagYFP-Fus, ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð° ÐµÐ³Ð¾ ÑÐºÐ·Ð¾Ð³ÐµÐ½Ð½Ð°Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð² ÐºÐ»ÐµÑÐºÐ°Ñ Ð¼Ð»ÐµÐºÐ¾Ð¿Ð¸ÑÐ°ÑÑÐ¸Ñ Ð²Ð¼ÐµÑÑÐµ Ñ Ð´ÑÑÐ³Ð¸Ð¼ ÑÐ¸Ð¼ÐµÑÐ½ÑÐ¼ Ð±ÐµÐ»ÐºÐ¾Ð¼ phyB-mCherry-Fus, Ð¸ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÐºÐ¾Ð½ÑÐ¾ÐºÐ°Ð»ÑÐ½Ð¾Ð¹ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸ Ð¸Ð·ÑÑÐµÐ½Ð° Ð¸Ñ Ð³ÐµÑÐµÑÐ¾Ð´Ð¸Ð¼ÐµÑÐ¸Ð·Ð°ÑÐ¸Ñ Ð¸ Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð½Ð° Ð¸Ñ Ð¾ÑÐ½Ð¾Ð²Ðµ ÐÐÐ. Ð ÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð»Ð¸, ÑÑÐ¾ Ð¾Ð±Ð»ÑÑÐµÐ½Ð¸Ðµ ÑÐ°ÑÑÐµÑÐ½Ð½ÑÐ¼ ÑÐ²ÐµÑÐ¾Ð¼ Ñ Ð´Ð»Ð¸Ð½Ð¾Ð¹ Ð²Ð¾Ð»Ð½Ñ 630 Ð½Ð¼ Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð»Ð¾ Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¸ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐµÐµ ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ðµ ÑÐ°Ð·Ð¼ÐµÑÐ¾Ð² ÐÐÐ. Ð¡Ð±Ð¾ÑÐºÐ° Ð¸ ÑÐ°Ð·Ð±Ð¾ÑÐºÐ° ÑÐ¾ÑÐ¾Ð¸Ð½Ð´ÑÑÐ¸ÑÑÐµÐ¼ÑÑ ÐÐÐ Ð² ÑÐ¸ÑÐ¾Ð¿Ð»Ð°Ð·Ð¼Ðµ ÐºÐ»ÐµÑÐ¾Ðº Ð¼Ð»ÐµÐºÐ¾Ð¿Ð¸ÑÐ°ÑÑÐ¸Ñ Ð¿Ð¾Ð´ÑÐ¸Ð½ÑÑÑÑÑ Ð·Ð°ÐºÐ¾Ð½Ð°Ð¼, Ð¿ÑÐ¸ÑÑÑÐ¸Ð¼ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾Ð¼Ñ ÑÐ°Ð·Ð¾Ð²Ð¾Ð¼Ñ ÑÐ°Ð·Ð´ÐµÐ»ÐµÐ½Ð¸Ñ Ð±Ð¸Ð¾Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ¾Ð². Ð Ð°Ð·Ð¼ÐµÑÑ ÐÐÐ, Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð½ÑÑ Ð¸Ð· phyB Ð¸ PIF6 Ð² ÐºÐ»ÐµÑÐºÐ°Ñ Ð¼Ð»ÐµÐºÐ¾Ð¿Ð¸ÑÐ°ÑÑÐ¸Ñ, ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²Ð¾Ð²Ð°Ð»Ð¸ ÑÐ°Ð·Ð¼ÐµÑÐ°Ð¼ ÑÐ°Ðº Ð½Ð°Ð·ÑÐ²Ð°ÐµÐ¼ÑÑ Â«ÑÐ°Ð½Ð½Ð¸ÑÂ» ÑÐ¾ÑÐ¾ÑÐµÐ»ÐµÑ Ð² ÐºÐ»ÐµÑÐºÐ°Ñ ÑÐ°ÑÑÐµÐ½Ð¸Ð¹. Ð­ÑÐ¸ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÑÑ Ð¿ÑÐµÐ´Ð¿Ð¾Ð»Ð¾Ð¶Ð¸ÑÑ, ÑÑÐ¾ Ð¿ÐµÑÐ²Ð°Ñ ÑÑÐ°Ð´Ð¸Ñ Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐ¾ÑÐ¾ÑÐµÐ»ÐµÑ Ñ ÑÐ°ÑÑÐµÐ½Ð¸Ð¹ Ð¼Ð¾Ð¶ÐµÑ Ð±ÑÑÑ Ð¾ÑÐ½Ð¾Ð²Ð°Ð½Ð° Ð½Ð° ÑÐ²ÐµÑÐ¾Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾Ð¼ ÑÐ°Ð·Ð¾Ð²Ð¾Ð¼ Ð¿ÐµÑÐµÑÐ¾Ð´Ðµ Ð¶Ð¸Ð´ÐºÐ¾ÑÑÑ-Ð¶Ð¸Ð´ÐºÐ¾ÑÑÑ Ð´Ð»Ñ PIF Ð¸ Ð´ÑÑÐ³Ð¸Ñ Ð±ÐµÐ»ÐºÐ¾Ð², ÐºÐ¾ÑÐ¾ÑÑÐµ Ð¼Ð¾Ð³ÑÑ ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐµÑÐºÐ¸ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¾Ð²Ð°ÑÑ Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾Ð¹ ÑÐ¾ÑÐ¼Ð¾Ð¹ phyB. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐ¸Ð¼ÐµÑÐ½ÑÐµ Ð±ÐµÐ»ÐºÐ¸ Ð² Ð¿ÑÐ¸Ð½ÑÐ¸Ð¿Ðµ Ð¼Ð¾Ð³ÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°ÑÑÑÑ Ð´Ð»Ñ ÑÐ¿ÑÐ°Ð²Ð»ÐµÐ½Ð¸Ñ ÑÐ±Ð¾ÑÐºÐ¾Ð¹ Ð¸ ÑÐ°Ð·Ð±Ð¾ÑÐºÐ¾Ð¹ ÑÐ¾ÑÐ¾Ð¸Ð½Ð´ÑÑÐ¸ÑÑÐµÐ¼ÑÑ ÐÐO Ð¸, ÑÐ°ÐºÐ¸Ð¼ Ð¾Ð±ÑÐ°Ð·Ð¾Ð¼, Ð´Ð»Ñ ÑÐµÐ³ÑÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð¿ÑÐ¾ÑÐµÑÑÐ¾Ð² Ð² ÐºÐ»ÐµÑÐºÐ°Ñ Ð¼Ð»ÐµÐºÐ¾Ð¿Ð¸ÑÐ°ÑÑÐ¸Ñ.
description: Plant photobodies are the membrane-less organelles (MLOs) that can be generated by protein-protein interactions between active form of phytochrome B (phyB) and phytochrome-interacting factors (PIFs). These organelles regulate plant photo-morphogenesis. In this work, the chimeric protein PIF6-TagYFP-Fus was developed, its and another developed chimeric proteins phyB-mCherry-Fus exogenous expression in mammalian cells was established, and their heterodimerization and the formation of NMOs based on them were studied using confocal fluorescence microscopy. Results showed that irradiation with diffused 630-nm light induced formation and subsequent increase in sizes of the MLOs. The assembly and disassembly of the photo-inducible MLOs in the mammalian cell cytoplasm obeyed the laws inherent in the concentration-dependent phase separation of biopolymers. The sizes of MLOs formed from phyB and PIF6 in mammalian cells corresponded to the sizes of the so-called âearlyâ photobodies in plant cells. These results suggested that the first step for the formation of plant photobodies might be based on the light-dependent liquid-liquid phase separation of PIFs and other proteins that can specifically interact with the active form of phyB. The developed chimeric proteins in principle can be used to control the assembly and disassembly of photo-inducible MLOs, and thereby to regulate various intracellular processes in mammalian cells.