Raw Data - Hypoxic Preconditioning Promotes Survivals of Human Adipocyte Mesenchymal Stem Cell via Expression of Prosurvival and Proangiogenic Biomarkers
Creators: name: Suryawan, I Gde Rurus
name: Pikir, Budi Susetyo
name: Rantam, Fedik Abdul
name: Kartika, Anudya
name: Nugraha, Ricardo Adrian
Publisher: name: figshare
Year: 2021
Subjects: subject: 110201 Cardiology (incl. Cardiovascular Diseases)
subject: FOS: Clinical medicine
subject: FOS: Clinical medicine
subject: 111601 Cell Physiology
subject: FOS: Basic medicine
subject: FOS: Basic medicine
subject: Stem Cells
subject: 100404 Regenerative Medicine (incl. Stem Cells and Tissue Engineering)
subject: FOS: Medical biotechnology
subject: FOS: Medical biotechnology

description: <i>Background</i>. Contributing factors for improved survival of human adipocytes mesenchymal stem cells (h-AMSCs) cultured through hypoxia preconditioning, in example apoptosis inhibition involving BCL2 and HSP27 expression, trigger signal expression (VEGF), SCF expression, OCT-4 expression, and CD44+ expression.<i>Objective</i>. To explain the mechanism and role of hypoxic preconditioning and the optimal duration of hypoxic preconditioning exposure to improve survival of h-AMSCs so that could it could be used as a benchmark for h-AMSCs culture strategy before transplantation.<i>Methods</i>. This study was an experimental laboratory explorative study (in vitro study) with hypoxic preconditioning in human-adipose mesenchymal stem cells (h-AMSCs) cultures. This research was conducted through 4 stages ;First, Isolation and h-AMSCs culture from adipose tissue of patient (human). Second is the characterization of h-AMSCs from adipose tissue by phenotype (Flowcytometry) through CD44+, CD90+ and CD45-expression before being pre-conditioned for hypoxic treatment. Third, the hypoxic preconditioning in h-AMSCs culture (in vitro) was performed with an oxygen concentration of 1% for 24, 48 and 72 hours. Fourth, observation of survival from h-AMSCs culture was tested on the role of CD44 +, VEGF, SCF, OCT-4, BCL2, HSP27 with Flowcytometry method and apoptotic inhibition by Tunnel Assay method.<i>Results</i>. The result of regression test showed that time difference had an effect on VEGF expression (<i>p</i>=0,000;<b>β</b>=-0,482) and hypoxia condition also influenced VEGF expression (<i>p</i>= 0,000; <b>β</b>=0,774). The result of path analysis showed that SCF had an effect on OCT-4 expression (<i>p</i>=0,000; <b>β</b>=0,985). The regression test results showed that time effects on HSP27 expression (<i>p</i>=0.000; <b>β</b>=0.398) and hypoxia precondition also affects HSP27 expression (<i>p</i>=0.000; <b>β</b>=0.847). Pathway analysis showed that BCL2 expression inhibited apoptosis (<i>p</i>=0.030; <b>β</b>=-0.442) and HSP27 expression also inhibited apoptosis (<i>p</i>=0,000; <b>β</b>=-0.487).<i>Conclusion</i>. In conclusion, hypoxic preconditioning of h-AMSC culture has proven to increase the expression of VEGF, SCF, OCT-4, and BCL2 and HSP27. This study demonstrated and explained the existence of a new mechanism of increased h-AMSC survival in cultures with hypoxic preconditioning (O2 1%) via VEGF, SCF, OCT-4, BCL2, and HSP 27. But CD 44+ did not play a role in the mechanism of survival improvement of human AMSC survival.