Additional file 3 of AsHSP26.8a, a creeping bentgrass small heat shock protein integrates different signaling pathways to modulate plant abiotic stress response
Creators: name: Sun, Xinbo
name: Zhu, Junfei
name: Li, Xin
name: Li, Zhigang
name: Han, Liebao
name: Luo, Hong
Publisher: name: figshare
Year: 2020
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: Plant Biology

description: Additional file 3: Figure S1. AsHSP26.8a nucleic acid sequence (A) and amino acid sequence (B) alignment with AsHSP26.8. Figure S2. Amino acid sequence alignment of AsHSP26.8a and other plant chloroplast localized (CP) sHSPs. Accession umbers of sHSPs are: Agrostis stolonifera HSP26.8a (KU353578); Aegilops kotschyi HSP26.8 (CAI96511); Triticum dicoccoides HSP26.4 (CAI96512); Hordeum vulgare HSP26 (AAB28590); Brachypodium distachyon sHSP (XP_003558381.1); Spartina alterniflora HSP27.1 (AFP96756.1); Zea mays HSP18 (CAM12752.1); Oryza sativa HSP26 (BAA78385.1). Figure S3. The phylogenetic relationship of the AsHSP26.8a with different classes of sHSPs from other plant species. Accession numbers of the sHSPs are: Arabidopsis thaliana HSP17.4 (X17293); Arabidopsis thaliana HSP17.6 (X16076); Oryza sativa HSP17.4 (D12635); Daucus carota HSP18.0 (X53852); Helianthus annuus HSP17.6 (X59701); Agrostis stolonifera HSP17 (KT272405); Triticum aestivum HSP16.9A (X13431); Oryza sativa HSP16.9A (X60820); Zea mays HSP16.9 (X65725); Solanum lycopersicum HSP17.8 (X56138); Glycine max HSP17.5 (M11318); Glycine max HSP17.6 (MI1317); Medicago sativa HSP18.1 (X58710); Pisum sativum HSP18.1 (M33899); Arabidopsis thaliana HSP22 (U11501); Glycine max HSP22 (X63198); Pisum sativum HSP22 (M33898); Arabidopsis thaliana HSP18.5 (816448); Oryza sativa HSP17.6 (113595340); Arabidopsis thaliana HSP15.7 (833746); Arabidopsis thaliana HSP26.5 (841687); Oxybasis rubra HSP23 (X15333); Arabidopsis thaliana HSP23.6 (828623); Oryza sativa HSP22 (4330786); Agrostis stolonifera HSP26.8a (KU353578); Triticum aestivum HSP26.6 (X58280); Zea mays HSP26 (L28712); Petunia x hybrida HSP21 (X54103); Arabidopsis thaliana HSP21 (X54102); Glycine max HSP22 (X07188); Pisum sativium HSP21 (X07187); Arabidopsis thaliana HSP15.4 (828276); Oryza sativa HSP18.8 (4343386); Arabidopsis thaliana HSP17.4 (841843); Oryza sativa HSP17.6B (4330933); Lilium longiflorum HSP17.6 (D21816); Zea mays HSP17.5 (X54076); Zea mays HSP17.8 (X54075); Triticum aestivum HSP17.3 (X58279); Arabidopsis thaliana HSP17.6 (X63443); Ipomoea nil HSP18.8 (M99430); Ipomoea nil HSP17.2 (M99429); Glycine max HSP17.9 (X07159); Pisum sativum HSP17.7 (M33901); Arabidopsis thaliana HSP21.7 (835555); Oryza sativa HSP22.2 (4339231). Figure S4. Gene ontology (GO) classification of the annotated unigenes or differentially expressed genes (DEGs). Unigenes with best BLAST hits were aligned to GO database. A total of 26,326 unigenes (red) and 261 DEGs (blue) were assigned to at least one GO term and grouped into three main GO categories (cellular component, molecular function and biological process) and 53 GO terms. Left Y-axis represents the percentages of unigenes or DEGs in each main category. Right Y-axis indicates the numbers of unigenes or DEGs in each GO term. Figure S5. Correlation assessment of a pair of biological replicates in wild type (WT) and AsHSP26.8a transgenic (TG) plants, respectively, used for RNA-seq. A, Pearson’s correlation coefficient between the two biological replicates of the wild type (E01 and E02) or AsHSP26.8a transgenic plants (E05 and E06). B and C, Correlation plots of a pair of biological replicates from wild type (E01 and E02), (B) and AsHSP26.8a transgenic line TG3 (E05 and E06) (C), respectively. Each dot in the plot represents a gene, denoting the log10 of the gene expression (FPKM) in the two replicate samples (x-axis and y-axis). The closer is the dot to the diagonal, the smaller is the difference in expression for the corresponding gene in the two replicates.