title: ÐÐ¾Ð´ÐµÐ»Ñ ÐºÐ¸Ð½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ð¹ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð²
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: gene kinetic expression model
Creators: name: Ð¢ÑÐ¸Ð½Ð¾Ð³Ð¸Ð½, ÐÐ½Ð´ÑÐµÐ¹
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸Ð¾Ð½Ð½ÑÐµ ÑÐ°ÐºÑÐ¾ÑÑ
ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð³ÐµÐ½Ð¾Ð²
ÐºÐ¸Ð½ÐµÑÐ¸ÑÐµÑÐºÐ°Ñ Ð¼Ð¾Ð´ÐµÐ»Ñ
Ð¾ÑÐµÐ½ÐºÐ° Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÐ¾Ð²
Ð½ÐµÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐµÑÐºÐ¾Ðµ ÑÐ²ÑÐ·ÑÐ²Ð°Ð½Ð¸Ðµ
transcription factors
gene expression
kinetic model
parameter estimation
non-specific association

description: Ð Ð°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¼Ð¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Ð¸ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸Ð¾Ð½Ð½ÑÑ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð² Ñ ÐÐÐ Ð½Ð° Ð¿ÑÐ¸Ð¼ÐµÑÐµ Ð³ÐµÐ½Ð° giant Ð² Ð³ÐµÐ½Ð¾Ð¼Ðµ Ð´ÑÐ¾Ð·Ð¾ÑÐ¸Ð»Ñ (Drosophila melanogaster) Ð¸ ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸Ð¾Ð½Ð½ÑÑ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð², ÑÐµÐ³ÑÐ»Ð¸ÑÑÑÑÐ¸Ñ ÑÐ¼Ð±ÑÐ¸Ð¾Ð³ÐµÐ½ÐµÐ·. ÐÑÐµÐ´Ð»Ð¾Ð¶ÐµÐ½Ð° Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð°Ñ Ð¼Ð¾Ð´ÐµÐ»Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸, ÐºÐ¾ÑÐ¾ÑÐ°Ñ ÑÑÐ¸ÑÑÐ²Ð°ÐµÑ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾ÑÑÑ Ð½ÐµÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐ²ÑÐ·ÑÐ²Ð°Ð½Ð¸Ñ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð² ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸Ð¸ Ð¸ ÐÐÐ. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð½ÐµÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐ²ÑÐ·ÑÐ²Ð°Ð½Ð¸Ñ Ñ ÐÐÐ Ð¼Ð¾Ð»ÐµÐºÑÐ»Ð° ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸Ð¾Ð½Ð½Ð¾Ð³Ð¾ ÑÐ°ÐºÑÐ¾ÑÐ° Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐµÑ Ð¿Ð¾Ð¸ÑÐº ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐ°Ð¹ÑÐ° Ð¿Ð¾ÑÐ°Ð´ÐºÐ¸ Ð² ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð¾Ð´Ð½Ð¾Ð¼ÐµÑÐ½Ð¾Ð¹ Ð´Ð¸ÑÑÑÐ·Ð¸Ð¸ (Â«ÑÐºÐ¾Ð»ÑÐ¶ÐµÐ½Ð¸ÑÂ») Ð¿Ð¾ ÐÐÐ. Ð Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½Ð¾Ð¹ Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð¿Ð¾ÑÐ²Ð»ÑÐµÑÑÑ Ð½Ð¾Ð²ÑÐ¹ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑ â ÑÐ°ÑÐ°ÐºÑÐµÑÐ½Ð°Ñ Ð´Ð»Ð¸Ð½Ð° ÑÐºÐ¾Ð»ÑÐ¶ÐµÐ½Ð¸Ñ Ð¿Ð¾ ÐÐÐ. ÐÐ»Ñ Ð½ÐµÑÐºÐ¾Ð»ÑÐºÐ¸Ñ Ð·Ð½Ð°ÑÐµÐ½Ð¸Ð¹ Ð´Ð»Ð¸Ð½Ñ ÑÐºÐ¾Ð»ÑÐ¶ÐµÐ½Ð¸Ñ Ð±ÑÐ»Ð° ÑÐ¸ÑÐ»ÐµÐ½Ð½Ð¾ ÑÐµÑÐµÐ½Ð° Ð¾Ð±ÑÐ°ÑÐ½Ð°Ñ Ð·Ð°Ð´Ð°ÑÐ° Ð¼Ð¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¸ Ð¾ÑÐµÐ½ÐµÐ½Ñ ÑÐ²Ð¾Ð±Ð¾Ð´Ð½ÑÐµ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÑ Ð² Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð¿Ð¾ Ð´Ð°Ð½Ð½ÑÐ¼ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð° giant Ð² ÑÐ¼Ð±ÑÐ¸Ð¾Ð½Ðµ Ð´ÑÐ¾Ð·Ð¾ÑÐ¸Ð»Ñ. Ð¡ÑÐ°Ð²Ð½ÐµÐ½Ð¸Ðµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð² ÑÐµÑÐµÐ½Ð¸Ñ Ð¾Ð±ÑÐ°ÑÐ½Ð¾Ð¹ Ð·Ð°Ð´Ð°ÑÐ¸ Ð² Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð¹ Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð¸ Ð² Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð±ÐµÐ· ÑÑÑÑÐ° Ð½ÐµÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐ²ÑÐ·ÑÐ²Ð°Ð½Ð¸Ñ Ð¿Ð¾Ð·Ð²Ð¾Ð»Ð¸Ð»Ð¾ ÑÐ´ÐµÐ»Ð°ÑÑ Ð²ÑÐ²Ð¾Ð´ Ð¾Ð± Ð¾ÑÑÑÑÑÑÐ²Ð¸Ð¸ Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼Ð¾ÑÑÐ¸ ÑÑÑÑÐ° Ð¾Ð´Ð½Ð¾Ð¼ÐµÑÐ½Ð¾Ð¹ Ð´Ð¸ÑÑÑÐ·Ð¸Ð¸ ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸Ð¾Ð½Ð½ÑÑ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð² Ð´Ð»Ñ Ð¼Ð¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Ð² ÑÐ°ÑÑÐ¼Ð¾ÑÑÐµÐ½Ð½Ð¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ðµ, ÑÑÐ¾, Ð¿Ð¾-Ð²Ð¸Ð´Ð¸Ð¼Ð¾Ð¼Ñ, ÑÐ²ÑÐ·Ð°Ð½Ð¾ Ñ Ð¾ÑÐ¾Ð±ÐµÐ½Ð½Ð¾ÑÑÑÐ¼Ð¸ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Ð² ÑÐ¾Ð´Ðµ ÑÐ°Ð½Ð½ÐµÐ³Ð¾ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ñ Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð¾Ð². Ð ÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð´Ð°ÑÑ Ð½Ð¾Ð²ÑÑ Ð¸Ð½ÑÐ¾ÑÐ¼Ð°ÑÐ¸Ñ Ð¾ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð°Ñ ÑÐµÐ³ÑÐ»ÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Ð² ÑÐ¼Ð±ÑÐ¸Ð¾Ð³ÐµÐ½ÐµÐ·Ðµ, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¼Ð¾Ð³ÑÑ Ð±ÑÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ Ð´Ð»Ñ ÑÐ¾ÑÐ¼ÑÐ»Ð¸ÑÐ¾Ð²ÐºÐ¸ Ð±Ð¾Ð»ÐµÐµ ÑÐ¾ÑÐ½ÑÑ Ð¼Ð¾Ð´ÐµÐ»ÐµÐ¹ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð².
description: The work is devoted to modeling gene expression and interaction of transcription factors with DNA on the example of the giant gene in the Drosophila melanogaster genome and transcription factors regulating embryogenesis. A modified model of expression that considers the possibility of non-specific binding of transcription and DNA factors is proposed. As a result of nonspecific binding to DNA, the transcription factor molecule searches for a specific landing site as a result of one-dimensional diffusion ("sliding") by DNA. A new parameter appears in the obtained model - the characteristic length of transcription factor sliding on DNA. For several values of sliding length, the inverse problem of modeling has been numerically solved and free parameters in the model have been estimated according to the data of giant gene expression in drosophila embryo. Comparison of results of the inverse problem solution in the modified model and in the model without considering non-specific binding allowed to draw a conclusion about non-existence of considering one-dimensional diffusion of transcription factors for modeling gene expression. The results of the researching provide new information on the mechanisms of gene regulation in embryogenesis and can also be used to formulate more accurate gene expression models.