cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
(400066) Rachel Melvill
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Nontopological Soliton in the Polyakov Quark Meson Model
Creators: name: Jin, Jinshuang
name: Mao, Hong
Publisher: name: arXiv
Year: 2015
Subjects: subject: High Energy Physics - Phenomenology (hep-ph)
subject: Nuclear Theory (nucl-th)
subject: FOS: Physical sciences

description: Within a mean field approximation, we study a nontopological soliton solution of the Polyakov quark-meson model in the presence of a fermionic vacuum term with two flavors at finite temperature and density. The profile of the effective potential exhibits a stable soliton solution below a critical temperature $T\leq T_χ^c$ for both the crossover and the first-order phase transitions, and these solutions are calculated here with appropriate boundary conditions. However, it is found that only if $T\leq T^c_d$,the energy of the soliton $M_N$ is less than the energy of the three free constituent quarks $3M_q$. As $T> T^c_d$, there is an instant delocalization phase transition from hadron matter to quark matter. The phase diagram together with the location of a critical end point (CEP) has been obtained in $T$ and $μ$ plane. We notice that two critical temperatures always satisfy $T^c_d\leq T_χ^c$. Finally, we present and compare the result of thermodynamic pressure at zero chemical potential with lattice data.
description: 17 pages, 9 figures, Accepted for publication in Physical Review C. arXiv admin note: text overlap with arXiv:1301.6227
Visualization of double-stranded RNAs from the myotonic dystrophy protein kinase gene and interactions with CUG-binding protein
Creators: name: Swanson, Maurice S.
name: Miller, Jill W.
name: Michalowski, Susan
name: Griffith, Jack
name: Urbinati, Carl R.
name: Paliouras, Miltiadis
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1999

description: Myotonic dystrophy (DM) is associated with a (CTG) (n) triplet repeat expansion in the 3'-untranslated region of the myotonic dystrophy protein kinase (DMPK) gene. Using electron microscopy, we visualized large RNAs containing up to 130 CUG repeats and studied the binding of purified CUG-binding protein (CUG-BP) to these RNAs. Electron microscopic examination revealed perfect double-stranded (ds)RNA segments whose lengths were that expected for duplex RNA. The RNA dominant mutation model for DM pathogenesis predicts that the expansion mutation acts at the RNA level by forming long dsRNAs that sequester certain RNA-binding proteins. To test this model, we examined the subcellular distribution and RNA-binding properties of CUG-BP. While previous studies have demonstrated that mutant DMPK transcripts accumu-late in nuclear foci, the localization pattern of CUG-BP in both normal and DM cells was similar. Although CUG-BP in nuclear extracts preferentially photocrosslinked to DMPK transcripts, this binding was not proportional to (CUG) (n) repeat size. Moreover, CUG-BP localized to the base of the RNA hairpin and not along the stem, as visualized by electron micro-scopy. These results provide the first visual evidence that the DM expansion forms an RNA hairpin structure and suggest that CUG-BP is unlikely to be a sequestered factor.
(400067) Robert Bell
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Supplementary material from Morphology and stable isotope analysis demonstrate different structuring of bat communities in rainforest and savannah habitats.
Creators: name: Monadjem, Ara
name: Kane, Adam
name: Taylor, Peter
name: Richards, Leigh R.
name: Hall, Grant
name: Woodborne, Stephan
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: Ecology
subject: FOS: Biological sciences

description: Bats play important ecological roles in tropical systems, yet how these communities are structured is still poorly understood. Our study explores the structure of African bat communities using morphological characters to define the morphospace occupied by these bats and stable isotope analysis to define their dietary niche breadth. We compared two communities, one in rainforest (Liberia) and one in savannah (South Africa), and asked whether the greater richness in the rainforest was due to more species ‘packing’ into the same morphospace and trophic space than bats from the savannah, or some other arrangement. In the rainforest, bats occupied a larger area in morphospace and species packing was higher than in the savannah; although this difference disappeared when comparing insectivorous bats only. There were also differences in morphospace occupied by different foraging groups (aerial, edge, clutter and fruitbat). Stable isotope analysis revealed that the range of δ13C values was almost double in rainforest than in savannah indicating a greater range of utilization of basal C3 and C4 resources in the former site, covering primary productivity from both these sources. The ranges in δ15N, however, were similar between the two habitats suggesting a similar number of trophic levels. Niche breadth, as defined by either standard ellipse area or convex hull, was greater for the bat community in rainforest than in savannah, with all four foraging groups having larger niche breadths in the former than the latter. The higher inter-species morphospace and niche breadth in forest bats suggest that species packing is not necessarily competitive. By employing morphometrics and stable isotope analysis, we have shown that the rainforest bat community packs more species in morphospace and uses a larger niche breadth than the one in savannah.
Enhanced delivery of antisense oligonucleotides with fluorophore-conjugated PAMAM dendrimers
Creators: name: Juliano, R. L.
name: Yoo, Hoon
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2000

description: PAMAM dendrimers are cationic polymers that have been used for the delivery of genes and oligonucleotides to cells. However, little is known about the behavior of dendrimer–nucleic acid complexes once they reach the cell interior. To pursue this issue, we prepared dendrimers conjugated with the fluorescent dye Oregon green 488. These were used in conjunction with oligonucleotides labeled with a red (TAMRA) fluorophore in order to visualize the sub-cellular distribution of the dendrimer–oligonucleotide complex and of its components by two-color digital fluorescence microscopy. The 2′-O-methyl antisense oligonucleotide sequence used in these studies was designed to correct splicing at an aberrant intron inserted into a luciferase reporter gene; thus effective delivery of the antisense agent results in the expression of the reporter gene product. The dendrimer–oligonucleotide complex remained associated during the process of uptake into vesicular compartments and eventual entry into the nucleus. Since the pharmacological activity of the antisense compound was manifest under these conditions, it suggests that the dendrimer–oligonucleotide complex is functionally active. A surprising result of these studies was that the Oregon green 488-conjugated dendrimer was a much better delivery agent for antisense compounds than unmodified dendrimer. This suggests that coupling of relatively hydrophobic small molecules to PAMAM dendrimers may provide a useful means of enhancing their capabilities as delivery agents for nucleic acids.
Modeling and knowledge acquisition processes using case-based inference
Publisher: name: Iranian Research Institute for Information Science and Technology (IranDoc)
Year: 2017

description: The method of acquisition and presentation of the organizational Process Knowledge has considered by many KM researches. In this research a model for process knowledge acquisition and presentation has been presented by using the approach of Case Base Reasoning. The validation of the presented model was evaluated by conducting an expert panel. Then a software has been developed based on the presented model and implemented in Eghtesad Novin Bank of Iran. In this company, based on the stages of the presented model, first the knowledge intensive processes has been identified, then the Process Knowledge was stored in a knowledge base in the format of problem/solution/consequent .The retrieval of the knowledge was done based on the similarity of the nearest neighbor algorithm. For validating of the implemented system, results of the system has compared by the results of the decision making of the expert of the process.
(400068) Margaret Todd
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
A model for the Acceptance of emerging technology âPacsâ in Iranâs hospitals
Publisher: name: Iranian Research Institute for Information Science and Technology (IranDoc)
Year: 2017

description: Development of Pacs is new opportunity for changing in the health system. Pacs including emerging technologies that provide accessibility to medical images via the network. Patient health records will be available through detailed video and film in everywhere and any time and with minimal cost. There is no trustee Pacs now in Iran and officials are looking to set up a center for the development Pacs. Under these conditions, identifying factors affecting the adoption of this technology will be very necessary and useful. This study is a correlation –descriptive research that was conducted in 1393. In this research TAM model is used for technology adoption. Variables affecting the technology extracted from the literature and these variables have been prioritized by the Delphi method and expert’s opinion. The model is analyzed by the structural equation method. Data’s was collected by a questionnaire including 16 items of the questionnaire that in this area was mined and were localized for Pacs technology. The target population was 130 of professionals. results of this study at medical centers using of Pacs technology confirmed, the effect of perceived financial costs on behavioral intention of Pacs (t-value = 12/31), the effect of compatibility on perceived usefulness by users (t-value = 8/74), the effect of image on perceived usefulness by users (t-value = 5/69), the effect of the image on the perceived ease of use (t-value = 12/74). According to the findings of this study, the factors affecting the acceptance Pacs were identified from the perspective of users' behavior. The results can be useful in planning of health system for the fair use of health cares and development and equipping of health centers.
R code from Morphology and stable isotope analysis demonstrate different structuring of bat communities in rainforest and savannah habitats
Creators: name: Monadjem, Ara
name: Kane, Adam
name: Taylor, Peter
name: Richards, Leigh R.
name: Hall, Grant
name: Woodborne, Stephan
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: Ecology
subject: FOS: Biological sciences

description: The relevant R code used in the calculation of various niche metrics
Extragenomic double-stranded DNA circles in yeast with linear mitochondrial genomes: potential involvement in telomere maintenance
Creators: name: Griffith, Jack D.
name: Tomaska, Lubomir
name: Makhov, Alexander M.
name: Nosek, Jozef
name: Pastorakova, Andrea
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2000

description: Although the typical mitochondrial DNA (mtDNA) is portrayed as a circular molecule, a large number of organisms contain linear mitochondrial genomes classified by their telomere structure. The class of mitochondrial telomeres identified in three yeast species, Candida parapsilosis, Pichia philodendra and Candida salmanticensis, is characterized by inverted terminal repeats each consisting of several tandemly repeating units and a 5′ single-stranded extension. The molecular mechanisms of the origin, replication and maintenance of this type of mitochondrial telomere remain unknown. While studying the replication of linear mtDNA of C.parapsilosis by 2-D gel electrophoresis distinct DNA fragments composed solely of mitochondrial telomeric sequences were detected and their properties were suggestive of a circular conformation. Electron microscopic analysis of these DNAs revealed the presence of highly supertwisted circular molecules which could be relaxed by DNase I. The minicircles fell into distinct categories based on length, corresponding to n × 0.75 kb (n = 1–7). Similar results were obtained with two other yeast species (P.philodendra and C.salmanticensis) which possess analogous telomeric structure.
Compare the user interface of digital libraries' websites between the developing and developed countries in content analysis method
Publisher: name: Iranian Research Institute for Information Science and Technology (IranDoc)
Year: 2017

description: Purpose: This study performed with goals of determining the Items in designing and developing the user interface of digital libraries' websites and to determine the best digital libraries' websites and discuss their advantages and disadvantages; to analyze and compare digital libraries' websites in developing countries with those in the developed countries. Methodology: to do so, 50 digital libraries' websites were selected by purposive sampling method. By analyzing the level of development of the countries in the sample regarding their digital libraries' websites, 12 websites were classified as belonging to developing and 38 countries to developed counties. Then, their content was studied by using a qualitative content analysis. The study was conducted by using a research-constructed checklist containing 12 main categories and 44 items, whose validity was decided by content validity method. The data was analyzed in SPSS (version 16). Findings: The results showed that in terms of “online resources”, “library collection,” and “navigation”, there is a significant relationship between the digital library' user interface design in both types of countries. Results: The items of “online public access catalogue (OPAC)” and “visits statistics” were observed in more developing countries’ digital libraries' websites. However, the item of “menu and submenus to introduce library' sections” was presented in more developed countries’ digital libraries' websites. Moreover, by analyzing the number of items in the selected websites, “American Memory” with 44 items, “International Children Digital Library” with 40 items, and “California” with 39 items were the best, and “Berkeley Sun Site” with 10 items was the worst website. Despite more and better quality digital libraries in developed countries, the quality of digital libraries websites in developing countries is considerable. In general, some of the newly established websites and lack of use the experience of specialists and librarians, and as a result, lack of information of site designers about users’ needs are some of the reasons for not designing appropriate digital libraries' websites.
Evaluation of Most Visited News Websites in Iran based on Machine learning
Publisher: name: Iranian Research Institute for Information Science and Technology (IranDoc)
Year: 2017

description: Success and effectiveness of websites is largely dependent on the quality of the website. The biggest share of the quality`s new concept is that the technical aspects of products and services combines with customers usage and understanding. Therefore, websites evaluation based on the maximum usage and perception of the customers is considered an important issue to announce to the related organizations the success of website. This ranking and evaluation should be performed in a special activity domain so that the first place of website rank determines among its other competitors. In this article achieving the information of websites is automatic and without the intervention of human so that the instant evaluation could be possible. In this study, one of the Multi criteria Decision-making methods called TOPSIS is used and the weights of the criteria have been achieved of the method entropy in the mentioned method. Eventually, according to the Alexa ranking report the 791 ranking news website have been obtained which have most visitors of the Iranian users, but on the other hand, just a numerical rank as a final output of websites evaluation can’t be very inconsistent with the purpose of competition between websites, so, these numerical ranking from TOPSIS method used as output in machine learing method for seprating websites from excellent to very poor in six categories as lables for training dataset in classification, instead of using manual lables achieved from experts and users’ opinion. For this classification, Machine learning techniques, including artificial neural network and support vector machine were used.
Investigate the relation between the media literacy and information literacy of students of communication science and information science and knowledge
Publisher: name: Iranian Research Institute for Information Science and Technology (IranDoc)
Year: 2017

description: The new millennium is called Information Age, in which information and communication technologies have been developed. The transfer from industrial society to information society has changed the form and level of education and information from those of the past times. In the past, literacy meant the ability of reading and writing, but today the meaning of literacy has been changed through the time and such a type of literacy is not enough to meet people’s needs in the industrial society of the 21st century. Today’s life requires media and information literacy especially for the students, whose duty is to research and who have a significant role in the development of their country from any perspective. This research aims to study the relation between the media literacy and information literacy of the students of the fields of communication science and information science and knowledge. This is an applied research in terms of its objective and uses a survey-correlation method. The statistical population of this research consists of the postgraduate students studying in the fields of study of information science and knowledge and communication science at Tehran University and Allameh Tabatabai University. The data required for this research were collected by a researcher-made questionnaire. The reliability of the questionnaire has been evaluated by Cronbach’s Alpha, which was equal to 0.936. The data were analyzed using descriptive and inferential statistic methods. The results showed that the level of media literacy and information literacy of students is desirable. There is a significant relationship between the economic status of students and their media literacy. However, the social status of students was directly related to their "ability to communicate" variable of media literacy. Also the Pearson correlation test showed a significant relationship between the variables of media literacy and information literacy.
23-bit Metaknowledge Template Towards Big Data Knowledge Discovery and Management
Creators: name: Bari, Nima
name: Vichr, Roman
name: Kowsari, Kamran
name: Berkovich, Simon Y.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Databases (cs.DB)
subject: Artificial Intelligence (cs.AI)
subject: Information Retrieval (cs.IR)
subject: Machine Learning (cs.LG)
subject: FOS: Computer and information sciences

description: The global influence of Big Data is not only growing but seemingly endless. The trend is leaning towards knowledge that is attained easily and quickly from massive pools of Big Data. Today we are living in the technological world that Dr. Usama Fayyad and his distinguished research fellows discussed in the introductory explanations of Knowledge Discovery in Databases (KDD) predicted nearly two decades ago. Indeed, they were precise in their outlook on Big Data analytics. In fact, the continued improvement of the interoperability of machine learning, statistics, database building and querying fused to create this increasingly popular science- Data Mining and Knowledge Discovery. The next generation computational theories are geared towards helping to extract insightful knowledge from even larger volumes of data at higher rates of speed. As the trend increases in popularity, the need for a highly adaptive solution for knowledge discovery will be necessary. In this research paper, we are introducing the investigation and development of 23 bit-questions for a Metaknowledge template for Big Data Processing and clustering purposes. This research aims to demonstrate the construction of this methodology and proves the validity and the beneficial utilization that brings Knowledge Discovery from Big Data.
description: IEEE Data Science and Advanced Analytics (DSAA'2014)
HMG I/Y regulates long-range enhancer-dependent transcription on DNA and chromatin by changes in DNA topology
Creators: name: Griffith, Jack D.
name: Michalowski, Susan
name: Sabnis, Rohit
name: Emerson, Beverly M.
name: Bagga, Rajesh
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2000

description: The nature of nuclear structures that are required to confer transcriptional regulation by distal enhancers is unknown. We show that long-range enhancer-dependent β-globin transcription is achieved in vitro upon addition of the DNA architectural protein HMG I/Y to affinity-enriched holo RNA polymerase II complexes. In this system, HMG I/Y represses promoter activity in the absence of an associated enhancer and strongly activates transcription in the presence of a distal enhancer. Importantly, nucleosome formation is neither necessary for long-range enhancer regulation in vitro nor sufficient without the addition of HMG I/Y. Thus, the modulation of DNA structure by HMG I/Y is a critical regulator of long-range enhancer function on both DNA and chromatin-assembled genes. Electron microscopic analysis reveals that HMG I/Y binds cooperatively to preferred DNA sites to generate distinct looped structures in the presence or absence of the β-globin enhancer. The formation of DNA topologies that enable distal enhancers to strongly regulate gene expression is an intrinsic property of HMG I/Y and naked DNA.
(400071) George David Todd Bell
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Biochemical characterization of the small isoform of Drosophila melanogaster RECQ5 helicase
Creators: name: Sekelsky, Jeff J.
name: Matson, Steven W.
name: Özsoy, A. Zeynep
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2001

description: Recently the gene encoding a member of the RecQ helicase family, RecQ5, was cloned from the fruit fly, Drosophila melanogaster [J.J.Sekelsky, M.H.Brodsky, G.M.Rubin and R.S.Hawley (1999) Nucleic Acids Res., 27, 3762–3769]. The Drosophila RecQ5 transcript is alternatively spliced, like its human counterpart, to yield three protein isoforms. Two of these isoforms are almost identical and have a predicted molecular weight of 54 kDa. The third isoform is larger and contains, in addition to the helicase domain shared by all three isoforms, a long highly charged C-terminal region. A small isoform of the Drosophila RecQ5 protein (RECQ5) has been expressed in Escherichia coli and purified. The purified protein is a single-stranded DNA-stimulated ATPase (dATPase) and a 3′→5′ DNA helicase. Hydrolysis of the nucleotide cofactor is required for unwinding activity and dATP supported the unwinding reaction better than other NTPs. The turnover number for the single-stranded DNA-stimulated dATPase activity was 1380 min–1, ~1.5-fold higher than that observed for the ATPase activity (900 min–1). The purified protein catalyzed unwinding of partial duplex substrates up to at least 93 bp, however, unwinding of an 89 bp blunt duplex substrate was not detected.
Mi2, an auto-antigen for dermatomyositis, is an ATP-dependent nucleosome remodeling factor
Creators: name: Wang, H. B.
name: Zhang, Y.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2001

description: Dynamic changes in chromatin structure play an important role in transcription regulation. Recent studies have revealed two mechanisms that alter chromatin structure. One involves ATP-dependent chromatin remodeling, and the other involves acetylation of the core histone tails. We have previously purified and characterized a multi-subunit protein complex, NuRD, which possesses both nucleosome remodeling and histone deacetylase activities. Despite extensive biochemical characterization of the complex, little is known about the functions of its individual components. In this study, we focused on Mi2, a component of the NuRD complex. We found that, similar to the native NuRD complex, recombinant Mi2 is a DNA-dependent, nucleosome-stimulated ATPase. Kinetic analysis of the ATP hydrolysis reaction indicated that the differential stimulation of the Mi2 ATPase by DNA and nucleosomes were primarily due to their differential effects on the turnover number of the reaction. Furthermore, we demonstrated that recombinant Mi2 is an efficient nucleosome remodeling factor when compared to that of the native NuRD complex. Our results define the biochemical function of Mi2 and set the stage for understanding the mechanism of nucleosome remodeling in a defined reconstituted system.
All-dielectric reciprocal bianisotropic nanoparticles
Creators: name: Alaee, Rasoul
name: Albooyeh, Mohammad
name: Rahimzadegan, Aso
name: Mirmoosa, Mohammad S.
name: Kivshar, Yuri S.
name: Rockstuhl, Carsten
Publisher: name: arXiv
Year: 2015
Subjects: subject: Optics (physics.optics)
subject: FOS: Physical sciences

description: The study of high-index dielectric nanoparticles currently attracts a lot of attention. They do not suffer from absorption but promise to provide control on the properties of light comparable to plasmonic nanoparticles. To further advance the field, it is important to identify versatile dielectric nanoparticles with unconventional properties. Here, we show that breaking the symmetry of an all-dielectric nanoparticle leads to a geometrically tunable magneto-electric coupling, i.e. an omega-type bianisotropy. The suggested nanoparticle exhibits different backscatterings and, as an interesting consequence, different optical scattering forces for opposite illumination directions. An array of such nanoparticles provides different reflection phases when illuminated from opposite directions. With a proper geometrical tuning, this bianisotropic nanoparticle is capable of providing a $2π$ phase change in the reflection spectrum while possessing a rather large and constant amplitude. This allows creating reflectarrays with near-perfect transmission out of the resonance band due to the absence of an usually employed metallic screen.
description: 7 pages, 6 figures
Nuclear antisense effects of neutral, anionic and cationic oligonucleotide analogs
Creators: name: Maier, Martin A.
name: Dillman, Jennifer
name: Kole, Ryszard
name: Sazani, Peter
name: Summerton, James
name: Kang, Shin Hong
name: Wei, Changfu
name: Manoharan, Muthiah
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2001

description: The antisense activity of oligomers with 2′-O-methyl (2′-O-Me) phosphorothioate, 2′-O-methoxyethyl (2′-O-MOE) phosphorothioate, morpholino and peptide nucleic acid (PNA) backbones was investigated using a splicing assay in which the modified oligonucleotides blocked aberrant and restored correct splicing of modified enhanced green fluorescent protein (EGFP) precursor to mRNA (pre-mRNA), generating properly translated EGFP. In this approach, antisense activity of each oligomer was directly proportional to up-regulation of the EGFP reporter. This provided a positive, quantitative readout for sequence-specific antisense effects of the oligomers in the nuclei of individual cells. Nuclear localization of fluorescent labeled oligomers confirmed validity of the functional assay. The results showed that the free uptake and the antisense efficacy of neutral morpholino derivatives and cationic PNA were much higher than that of negatively charged 2′-O-Me and 2′-O-MOE congeners. The effects of the PNA oligomers were observed to be dependent on the number of l-lysine (Lys) residues at the C-terminus. The experiments suggest that the PNA containing Lys was taken up by a mechanism similar to that of cell-penetrating homeodomain proteins and that the Lys tail enhanced intracellular accumulation of PNA oligomer without affecting its ability to reach and hybridize to the target sequence.
Global genome removal of thymine glycol in Escherichia coli requires endonuclease III but the persistence of processed repair intermediates rather than thymine glycol correlates with cellular sensitivity to high doses of hydrogen peroxide
Creators: name: Alanazi, Mohammed
name: Mellon, Isabel
name: Leadon, Steven A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2002

description: Using a monoclonal antibody that specifically recognizes thymine glycol (Tg) in DNA, we measured the kinetics of the removal of Tg from the genomes of wild-type and repair gene mutant strains of Escherichia coli treated with hydrogen peroxide. Tg is rapidly and efficiently removed from the total genomes of repair-proficient cells in vivo and the removal of Tg is completely dependent on the nth gene that encodes the endonuclease III glycosylase. Hence, it appears that little redundancy in the repair of Tg occurs in vivo, at least under the conditions used here. Moreover, previous studies have found that nth mutants are not sensitive to killing by hydrogen peroxide but xth mutant strains (deficient in the major AP endonuclease, exonuclease III) are sensitive. We find that cell death correlates with the persistence of single-strand breaks rather than the persistence of Tg. We attempted to measure transcription-coupled removal of Tg in the lactose operon using the Tg-specific monoclonal antibody in an immunoprecipitation approach but were not successful in achieving reproducible results. Furthermore, the analysis of transcription-coupled repair in the lactose operon is complicated by potent inhibition of β-galactosidase expression by hydrogen peroxide.
Novel Metaknowledge-based Processing Technique for Multimedia Big Data clustering challenges
Creators: name: Bari, Nima
name: Vichr, Roman
name: Kowsari, Kamran
name: Berkovich, Simon Y.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Databases (cs.DB)
subject: Artificial Intelligence (cs.AI)
subject: Information Retrieval (cs.IR)
subject: Multimedia (cs.MM)
subject: FOS: Computer and information sciences

description: Past research has challenged us with the task of showing relational patterns between text-based data and then clustering for predictive analysis using Golay Code technique. We focus on a novel approach to extract metaknowledge in multimedia datasets. Our collaboration has been an on-going task of studying the relational patterns between datapoints based on metafeatures extracted from metaknowledge in multimedia datasets. Those selected are significant to suit the mining technique we applied, Golay Code algorithm. In this research paper we summarize findings in optimization of metaknowledge representation for 23-bit representation of structured and unstructured multimedia data in order to
description: IEEE Multimedia Big Data (BigMM 2015)
(400064) Edmund Baxter
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
(400061) William Boyd Baxter
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Interactions between p53, hMSH2-hMSH6 and HMG I(Y) on Holliday junctions and bulged bases
Creators: name: Griffith, Jack D.
name: Subramanian, Deepa
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2002

description: The ability of the tumor suppressor protein, p53, to recognize certain types of DNA lesions may represent one of the mechanisms by which this protein modulates cellular response to DNA damage. p53 DNA binding properties are regulated by several factors, such as post-translational modifications including phosphorylation and acetylation, regulation by its own C-terminal domain and interactions with other cellular proteins. Substrates resembling Holliday junctions and extra base bulges were used to study the effect of three nuclear proteins, HMG-1, HMG I(Y) and hMSH2–hMSH6, on the lesion binding properties of p53. Gel retardation assays revealed that the three proteins had varying effects on p53 binding to these substrates. HMG-1 did not influence p53 binding to Holliday junctions or 3-cytosine bulges. HMG I(Y) rapidly dissociated p53 complexes with Holliday junctions but not 3-cytosine bulges. Finally, the mismatch repair protein complex, hMSH2–hMSH6, enhanced p53 binding to both substrates by 3–4-fold. Together, these results demonstrate that p53 DNA binding activity is highly influenced by the presence of other proteins, some having a dominant effect while others have a negative effect.
Additional file 1 of Cardiac reverse remodeling in primary mitral regurgitation: mitral valve replacement vs. mitral valve repair
Creators: name: Craven, Thomas P.
name: Chew, Pei G.
name: Dobson, Laura E.
name: Gorecka, Miroslawa
name: Parent, Martine
name: Brown, Louise A. E.
name: Saunderson, Christopher E. D.
name: Das, Arka
name: Chowdhary, Amrit
name: Jex, Nicholas
name: Higgins, David M.
name: Dall’Armellina, Erica
name: Levelt, Eylem
name: Schlosshan, Dominik
name: Swoboda, Peter P.
name: Plein, Sven
name: Greenwood, John P.
Publisher: name: figshare
Year: 2023
Subjects: subject: Space Science
subject: Medicine
subject: Cell Biology
subject: Physiology
subject: FOS: Biological sciences
subject: Biotechnology

description: Additional file 1. Operation variable comparisons between surgical groups. Surgical variables between groups and differences between groups when mitral valve replacement group was divided into those that received direct replacement and had mitral valve replacement after attempted repair.
(400063) Ann Baxter
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Commemorating heroes and martyrs in a Chinese elementary school: An exploration of the Qingming Sacrificial Ritual from the perspective of educational anthropology
Creators: name: Wang, Yumeng
Publisher: name: TU Dortmund
Year: 2022
Subjects: subject: Educational anthropology
subject: School ritual
subject: Ethnography
subject: Chinese education
subject: 370
subject: Pädagogische Anthropologie
subject: Ritual
subject: Schule
subject: China

description: Ritual is a classic research topic in anthropology. This study focuses on the Qingming Sacrificial Ritual in a primary school located in Beijing, China, under the theoretical framework of German educational anthropology. This empirical research is conducted under focused ethnography and documentary method. The Qingming Sacrificial Ritual is a modern expression of ancient Chinese sacrificial rituals，which originated from ancient Chinese sacrificial culture which is traditional ethical culture centered on filial piety in traditional Chinese Confucianism. Through the lens of Qingming Sacrificial Ritual, this research shows Chinese history, culture, society and school education to German and European readers on a more multi-perspective basis. As this dissertation focuses on Qingming Sacrificial Ritual in a Chinese primary school, a model of Chinese school ritual practice is also constructed through an in-depth description and analysis of the preparation, organization and performance of ritual, revealing the key factors that influence the formation of Chinese school ritual, including educational policies, educational system, school culture etc. It also provides international readers with basic understanding of elementary education in China. From methodology perspective, a policy logic analysis model of ritual research is proposed, developing a new analytical perspective for international ritual research. The focus on “ritual expert” offers a new direction of informant for researchers who want to gain a deeper understanding of Chinese school rituals through ethnography and fieldwork, providing a new direction for data collection.
(400016) Elspeth [Anderson]
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Ku heterodimer binds to both ends of the Werner protein and functional interaction occurs at the Werner N-terminus
Creators: name: Ramsden, Dale A.
name: Karmakar, Parimal
name: Snowden, Carey M.
name: Bohr, Vilhelm A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2002

description: The human Werner syndrome protein, WRN, is a member of the RecQ helicase family and contains 3′→5′ helicase and 3′→5′ exonuclease activities. Recently, we showed that the exonuclease activity of WRN is greatly stimulated by the human Ku heterodimer protein. We have now mapped this interaction physically and functionally. The Ku70 subunit specifically interacts with the N-terminus (amino acids 1–368) of WRN, while the Ku80 subunit interacts with its C-terminus (amino acids 940– 1432). Binding between Ku70 and the N-terminus of WRN (amino acids 1–368) is sufficient for stimulation of WRN exonuclease activity. A mutant Ku heterodimer of full-length Ku80 and truncated Ku70 (amino acids 430–542) interacts with C-WRN but not with N-WRN and cannot stimulate WRN exonuclease activity. This emphasizes the functional significance of the interaction between the N-terminus of WRN and Ku70. The interaction between Ku80 and the C-terminus of WRN may modulate some other, as yet unknown, function. The strong interaction between Ku and WRN suggests that these two proteins function together in one or more pathways of DNA metabolism.
Analysis of helicase activity and substrate specificity of Drosophila RECQ5
Creators: name: Matson, Steven W.
name: Ragonese, Heather M.
name: Özsoy, A. Zeynep
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2003

description: RecQ5 is one of five RecQ helicase homologs identified in humans. Three of the human RecQ homologs (BLM, WRN and RTS) have been linked to autosomal recessive human genetic disorders (Bloom syndrome, Werner syndrome and Rothmund–Thomson syndrome, respectively) that display increased genomic instability and cause elevated levels of cancers in addition to other symptoms. To understand the role of RecQ helicases in maintaining genomic stability, the WRN, BLM and Escherichia coli RecQ helicases have been characterized in terms of their DNA substrate specificity. However, little is known about other members of the RecQ family. Here we show that Drosophila RECQ5 helicase is a structure-specific DNA helicase like the other RecQ helicases biochemically characterized so far, although the substrate specificity is not identical to that of WRN and BLM helicases. Drosophila RECQ5 helicase is capable of unwinding 3′ Flap, three-way junction, fork and three-strand junction substrates at lower protein concentrations compared to 5′ Flap, 12 nt bubble and synthetic Holliday junction structures, which can be unwound efficiently by WRN and BLM.
Design of artificial transcription factors to selectively regulate the pro-apoptotic bax gene
Creators: name: Ye, D.
name: Fisher, M.
name: Juliano, R. L.
name: Falke, D.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2003

description: The tumor suppressor p53 is the most commonly mutated gene in human cancers. Active p53 is able to stimulate the transcription of a variety of genes including the pro-apoptotic gene bax, as well as p21, a cell cycle regulator. In this study we produced novel zinc finger transcription factors that would selectively increase the expression of bax, but not of other p53 targets. Reporter gene assays in p53-negative Saos-2 cells showed that the novel zinc finger proteins stimulated transcription driven by a minimal bax promoter, but not that driven by a minimal p21 promoter. Moreover, electromobility shift assays demonstrated that the novel transcription factors could bind the bax promoter sequence with high affinity and selectivity. Expression of a five zinc finger protein (5ZFAV) in COS-7 cells resulted in an increase in Bax protein levels, indicating that this novel transcription factor could act on endogenous gene expression. Expression of 5ZFAV also drastically reduced Saos-2 cell survival; this effect could be reversed by the general caspase inhibitor B-D-FMK. These data suggest that 5ZFAV is able to induce apoptosis through increased Bax expression. Further, while expression of 5ZFAV in p53-deficient Saos-2 cells reduced cell survival, there was little effect on U-2 OS cells which have wild-type p53. Thus the selective induction of the pro-apoptotic bax gene may be a valuable adjunct to cancer chemotherapy by diminishing survival of p53-deficient tumor cells.
Effects of sequence on repeat expansion during DNA replication
Creators: name: Heidenfelder, Brooke L.
name: Topal, Michael D.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2003

description: Small DNA repeat tracts are located throughout the human genome. The tracts are unstable, and expansions of certain repeat sequences cause neuromuscular disease. DNA expansions appear to be associated with lagging-strand DNA synthesis and DNA repair. At some sites of repeat expansion, e.g. the myotonic dystrophy type 2 (DM2) tetranucleotide repeat expansion site, more than one repeat tract with similar sequences lie side by side. Only one of the DM2 repeat tracts, however, is found to expand. Thus, DNA base sequence is a possible factor in repeat tract expansion. Here we determined the expansion potential, during DNA replication by human DNA polymerase β, of several tetranucleotide repeat tracts in which the repeat units varied by one or more bases. The results show that subtle changes, such as switching T for C in a tetranucleotide repeat, can have dramatic consequences on the ability of the nascent-strand repeat tract to expand during DNA replication. We also determined the relative stabilities of self-annealed 100mer repeats by melting-curve analysis. The relative stabilities did not correlate with the relative potentials of the analogous repeats for expansion during DNA replication, suggesting that hairpin formation is not required for expansion during DNA replication.