cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Protocolo de Coleta de PCCU na Atenção Primária
Creators: name: Gobbo, Aline
name: Ribeiro, Andressa Karoline Pinto de Lima
name: Leal, Deoclemar Balieiro Diniz
name: Caldas, Samantha Pereira
Publisher: name: Zenodo
Year: 2023

description: Protocolo de Coleta de PCCU na Atenção Primária do Munícipio de Belém
Functional Characterization of the Multidomain F Plasmid TraI Relaxase-Helicase
Creators: name: Nash, Rebekah P.
name: Redinbo, Matthew R.
name: Miley, Michael J.
name: McNamara, Dan E.
name: Cheng, Yuan
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: TraI, a bifunctional enzyme containing relaxase and helicase activities, initiates and drives the conjugative transfer of the Escherichia coli F plasmid. Here, we examined the structure and function of the TraI helicase. We show that TraI binds to single-stranded DNA (ssDNA) with a site size of ∼25 nucleotides, which is significantly longer than the site size of other known superfamily I helicases. Low cooperativity was observed with the binding of TraI to ssDNA, and a double-stranded DNA-binding site was identified within the N-terminal region of TraI 1–858, outside the core helicase motifs of TraI. We have revealed that the affinity of TraI for DNA is negatively correlated with the ionic strength of the solution. The binding of AMPPNP or ADP results in a 3-fold increase in the affinity of TraI for ssDNA. Moreover, TraI prefers to bind ssDNA oligomers containing a single type of base. Finally, we elucidated the solution structure of TraI using small angle x-ray scattering. TraI exhibits an ellipsoidal shape in solution with four domains aligning along one axis. Taken together, these data result in the assembly of a model for the multidomain helicase activity of TraI.
Functional Reconstitution of an Atypical G Protein Heterotrimer and Regulator of G Protein Signaling Protein (RGS1) from Arabidopsis thaliana
Creators: name: Temple, Brenda R.S.
name: Jones, Janice C.
name: Dohlman, Henrik G.
name: Jones, Alan M.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: It has long been known that animal heterotrimeric Gαβγ proteins are activated by cell-surface receptors that promote GTP binding to the Gα subunit and dissociation of the heterotrimer. In contrast, the Gα protein from Arabidopsis thaliana (AtGPA1) can activate itself without a receptor or other exchange factor. It is unknown how AtGPA1 is regulated by Gβγ and the RGS (regulator of G protein signaling) protein AtRGS1, which is comprised of an RGS domain fused to a receptor-like domain. To better understand the cycle of G protein activation and inactivation in plants, we purified and reconstituted AtGPA1, full-length AtRGS1, and two putative Gβγ dimers. We show that the Arabidopsis Gα protein binds to its cognate Gβγ dimer directly and in a nucleotide-dependent manner. Although animal Gβγ dimers inhibit GTP binding to the Gα subunit, AtGPA1 retains fast activation in the presence of its cognate Gβγ dimer. We show further that the full-length AtRGS1 protein accelerates GTP hydrolysis and thereby counteracts the fast nucleotide exchange rate of AtGPA1. Finally, we show that AtGPA1 is less stable in complex with GDP than in complex with GTP or the Gβγ dimer. Molecular dynamics simulations and biophysical studies reveal that altered stability is likely due to increased dynamic motion in the N-terminal α-helix and Switch II of AtGPA1. Thus, despite profound differences in the mechanisms of activation, the Arabidopsis G protein is readily inactivated by its cognate RGS protein and forms a stable, GDP-bound, heterotrimeric complex similar to that found in animals.
Находки iNaturalist как источник данных для изучения биоразнообразия России и их качество
Creators: name: Shashkov, Maxim
Publisher: name: Zenodo
Year: 2020
Subjects: iNaturalist; citizen science; data quality

description: В настоящее время больше половины всех находок, опубликованных в GBIF, происходят из систем «любительской науки» (Citizen Science), среди которых одной из крупнейших является iNaturalist. Соответствующий набор данных является восьмым по числу находок в GBIF (iNaturalist.org, 2020) и продолжает быстро расти. Набирает популярность iNaturalist и в нашей стране. На 14 февраля 2020 года для территории России представлено 505 тыс. находок более чем 17 тыс. видов, из которых около 81 % «исследовательского уровня», т.е. имеют корректную дату наблюдения, географическую привязку и подтвержденное участниками системы определение.
Using $G_0W_0$ Level Alignment to Identify Catechol's Structure on TiO$_2$(110)
Creators: name: Mowbray, Duncan J.
name: Migani, Annapaola
Publisher: name: arXiv
Year: 2015
Subjects: subject: Materials Science (cond-mat.mtrl-sci)
subject: FOS: Physical sciences

description: We perform state-of-the-art calculations for a prototypical dye sensitized solar cell: catechol on rutile TiO$_2$(110). Catechol is often used as an anchoring group for larger more complex organic and inorganic dyes on TiO$_2$ and forms a type II heterojunctions on TiO$_2$(110). In particular, we compare quasiparticle (QP) $G_0W_0$ with hybrid exchange correlation functional (HSE) density functional theory (DFT) calculations for the catechol-rutile TiO$_2$(110) interface. In so doing, we provide a theoretical interpretation of ultraviolet photoemission spectroscopy (UPS) and inverse photoemission spectroscopy (IPES) experiments for this prototypical system. Specifically, we demonstrate that the position, presence, and intensity of peaks associated with catechol's HOMO, intermolecular OH$-$O bonds, and interfacial hydrogen bonds to the surface bridging O atoms (O$_{br}$H$-$C and O$_{br}$H$-$O) may be used to fingerprint deprotonation of catechol's OH anchoring groups. Furthermore, our results suggest deprotonation of these groups, while being nearly isoenergetic at high coverages, may significantly increase the photovoltaic efficiency of catechol$-$TiO$_2$(110) interfaces.
description: 7 pages, 4 figures, corrected table 1
title: Quantification of cardiac subvolume dose in breast cancer patients undergoing left sided tangential radiotherapy
Creators: name: Tang, Simon
Publisher: name: The Royal Australian and New Zealand College of Radiologists
Year: 2017
Subjects: subject: Breast
subject: Cardiac
subject: Cardiovascular system
subject: CT
subject: Treatment effects
subject: Toxicity

description: Purpose Methods and materials Results Conclusion Personal information References
description: Purpose: As breast cancer treatment and subsequent survival improve, toxicities of therapy have increased in their importance. A study of 2168 women who underwent radiotherapy for breast cancer found a 7.4% increase in rate of major coronary artery events...
Gain in Transcriptional Activity by Primate-specific Coevolution of Melanoma Antigen-A11 and Its Interaction Site in Androgen Receptor
Creators: name: Minges, John T.
name: Liu, Qiang
name: Blackwelder, Amanda J.
name: Wilson, Elizabeth M.
name: Su, Shifeng
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: Male sex development and growth occur in response to high affinity androgen binding to the androgen receptor (AR). In contrast to complete amino acid sequence conservation in the AR DNA and ligand binding domains among mammals, a primate-specific difference in the AR NH2-terminal region that regulates the NH2- and carboxyl-terminal (N/C) interaction enables direct binding to melanoma antigen-A11 (MAGE-11), an AR coregulator that is also primate-specific. Human, mouse, and rat AR share the same NH2-terminal 23FQNLF27 sequence that mediates the androgen-dependent N/C interaction. However, the mouse and rat AR FXXLF motif is flanked by Ala33 that evolved to Val33 in primates. Human AR Val33 was required to interact directly with MAGE-11 and for the inhibitory effect of the AR N/C interaction on activation function 2 that was relieved by MAGE-11. The functional importance of MAGE-11 was indicated by decreased human AR regulation of an androgen-dependent endogenous gene using lentivirus short hairpin RNAs and by the greater transcriptional strength of human compared with mouse AR. MAGE-11 increased progesterone and glucocorticoid receptor activity independently of binding an FXXLF motif by interacting with p300 and p160 coactivators. We conclude that the coevolution of the AR NH2-terminal sequence and MAGE-11 expression among primates provides increased regulatory control over activation domain dominance. Primate-specific expression of MAGE-11 results in greater steroid receptor transcriptional activity through direct interactions with the human AR FXXLF motif region and indirectly through steroid receptor-associated p300 and p160 coactivators.
(3981) John Sinclair
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Histidyl-tRNA Synthetase Urzymes: CLASS I AND II AMINOACYL tRNA SYNTHETASE URZYMES HAVE COMPARABLE CATALYTIC ACTIVITIES FOR COGNATE AMINO ACID ACTIVATION
Creators: name: Carter Jr., Charles W.
name: Li, Li
name: Weinreb, Violetta
name: Francklyn, Christopher
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: Four minimal (119 - 145 residue) active site fragments of Escherichia coli Class II histidyl-tRNA synthetase were constructed, expressed as maltose-binding protein fusions, and assayed for histidine activation as fusion proteins and after TEV cleavage, using the 32PPi exchange assay. All contain conserved Motifs 1 and 2. Two contain an N-terminal extension of Motif 1 and two contain Motif 3. Five experimental results argue strongly for the authenticity of the observed catalytic activities: (i) active site titration experiments showing high (∼0.1–0.55) fractions of active molecules, (ii) release of cryptic activity by TEV cleavage of the fusion proteins, (iii) reduced activity associated with an active site mutation, (iv) quantitative attribution of increased catalytic activity to the intrinsic effects of Motif 3, the N-terminal extension and their synergistic effect, and (v) significantly altered Km values for both ATP and histidine substrates. It is therefore plausible that neither the insertion domain nor Motif 3 were essential for catalytic activity in the earliest Class II aminoacyl-tRNA synthetases. The mean rate enhancement of all four cleaved constructs is ∼109 times that of the estimated uncatalyzed rate. As observed for the tryptophanyl-tRNA synthetase (TrpRS) Urzyme, these fragments bind ATP tightly but have reduced affinity for cognate amino acids. These fragments thus likely represent Urzymes (Ur = primitive, original, earliest + enzyme) comparable in size and catalytic activity and coded by sequences proposed to be antisense to that coding the previously described Class I TrpRS Urzyme. Their catalytic activities provide metrics for experimental recapitulation of very early evolutionary events.
Homeostasis of Extracellular ATP in Human Erythrocytes
Creators: name: Lazarowski, Eduardo R.
name: Montalbetti, Nicolas
name: Schwarzbaum, Pablo J.
name: Leal Denis, Maria F.
name: Kobatake, Eiry
name: Pignataros, Omar P.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: We explored the intra- and extracellular processes governing the kinetics of extracellular ATP (ATPe) in human erythrocytes stimulated with agents that increase cAMP. Using the luciferin-luciferase reaction in off-line luminometry we found both direct adenylyl cyclase activation by forskolin and indirect activation through β-adrenergic stimulation with isoproterenol-enhanced [ATP]e in a concentration-dependent manner. A mixture (3V) containing a combination of these agents and the phosphodiesterase inhibitor papaverine activated ATP release, leading to a 3-fold increase in [ATP]e, and caused increases in cAMP concentration (3-fold for forskolin + papaverine, and 10-fold for 3V). The pannexin 1 inhibitor carbenoxolone and a pannexin 1 blocking peptide (10Panx1) decreased [ATP]e by 75–84%. The residual efflux of ATP resulted from unavoidable mechanical perturbations stimulating a novel, carbenoxolone-insensitive pathway. In real-time luminometry experiments using soluble luciferase, addition of 3V led to an acute increase in [ATP]e to a constant value of ∼1 pmol × (106 cells)−1. A similar treatment using a surface attached luciferase (proA-luc) triggered a rapid accumulation of surface ATP levels to a peak concentration of 2.4 pmol × (106 cells)−1, followed by a slower exponential decay (t½ = 3.7 min) to a constant value of 1.3 pmol × (106 cells)−1. Both for soluble luciferase and proA-luc, ATP efflux was fully blocked by carbenoxolone, pointing to a 3V-induced mechanism of ATP release mediated by pannexin 1. Ecto-ATPase activity was extremely low (∼28 fmol × (106 cells min)−1), but nevertheless physiologically relevant considering the high density of erythrocytes in human blood.
Reproductive biology of the spider crab Maja squinado (Herbst, 1788) (Decapoda: Majidae) in the Gulf of Gabès (Tunisia, Central Mediterranean)
Creators: name: BAKLOUTI, Sonia
name: BEN ABDALLAH BEN HADJ HAMIDA, Olfa
name: JARBOUI, Othman
Publisher: name: Station Biologique de Roscoff (SBR)
Year: 2015

description: The spider crab Maja squinado (Herbst, 1788) is an endangered Mediterranean species. Here, we characterized the reproductive biology of M. squinado, from the Gulf of Gabés, since no information on its reproductive parameters in Tunisian waters has been assessed previously. Sex ratio, reproductive cycle and size at first sexual maturity were studied in 770 individuals (292 females and 478 males) caught monthly in shallow and deep waters. The overall samples presented a carapace length (CL) ranging between 22.5 and 87 mm (62.07% males and 37.92% females). Proportions of both sexes varied depending on size and season, the majority of individuals ≤ 52 mm CL were females and the males were predominant in spring and winter. The size at which 50% of the population reached maturity was 52.17 ± 0.79 and 54.31 ± 0.45 mm CL for females and males, respectively. Monthly changes in the gonadosomatic and hepatosomatic indices and seasonal changes in microscopic characteristics of the gonads of both sexes showed that the reproductive activity was greatest in autumn and winter with a peak in November. M. squinado exhibited a short resting phase that occurred simultaneously in females and males from April to September.
Lon Peptidase 1 (LONP1)-dependent Breakdown of Mitochondrial 5-Aminolevulinic Acid Synthase Protein by Heme in Human Liver Cells
Creators: name: Tian, Qing
name: Hou, Weihong
name: Bonkovsky, Herbert L.
name: Li, Ting
name: Zheng, Jianyu
name: Schrum, Laura W.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: 5-Aminolevulinic acid synthase (ALAS-1) is the first rate controlling enzyme that controls cellular heme biosynthesis. Negative feedback regulation of ALAS-1 by the end product heme is well documented and provides the foundation for heme treatment of acute porphyrias, a group of diseases caused by genetic defects in the heme biosynthesis pathway and exacerbated by controlled up-regulation of ALAS-1. Heme is known to affect ALAS-1 activity by repressing gene transcription, accelerating mRNA degradation, and impeding pre-ALAS-1 mitochondrial translocation. In the current study, we examined the effect of heme on the rate of mature ALAS-1 protein turnover in human cells and tissues and explored the mediator involved in this new regulatory mechanism. We found that heme and other metalloporphyrins such as CoPP and CrPP decreased mitochondrial ALAS-1 protein through proteolysis. This degradative effect cannot be emulated by iron or free protoporphyrin, two major chemical components of the heme ring, and is independent of oxidative stress. Down-regulating the activity of mitochondrial LONP1, an ATP-dependent protease that controls the selective turnover of mitochondrial matrix proteins, with potent inhibitors and specific siRNA diminished the negative effect of heme on mitochondrial ALAS-1. Therefore, our data support the existence of a conserved heme feedback regulatory mechanism that functions on the mature form of ALAS-1 protein through the activity of a mitochondrial proteolytic system.
Explicit Bounds for the Pseudospectra of Various Classes of Matrices and Operators
Creators: name: Gong, Feixue
name: Meyerson, Olivia
name: Meza, Jeremy
name: Stoiciu, Mihai
name: Ward, Abigail
Publisher: name: arXiv
Year: 2015
Subjects: subject: Spectral Theory (math.SP)
subject: Numerical Analysis (math.NA)
subject: FOS: Mathematics
subject: 15A18, 47A10, 65F15, 15A60

description: We study the $ε$-pseudospectra $σ_ε(A)$ of square matrices $A \in \mathbb{C}^{N \times N}$. We give a complete characterization of the $ε$-pseudospectrum of any $2 \times 2$ matrix and describe the asymptotic behavior (as $ε\to 0$) of $σ_ε(A)$ for any square matrix $A$. We also present explicit upper and lower bounds for the $ε$-pseudospectra of bidiagonal matrices, as well as for finite rank operators.
description: 22 pages, 6 figures
All-loop order critical exponents for massless scalar field theory with Lorentz violation in the BPHZ method
Creators: name: Carvalho, Paulo R. S.
Publisher: name: arXiv
Year: 2015
Subjects: subject: High Energy Physics - Theory (hep-th)
subject: Statistical Mechanics (cond-mat.stat-mech)
subject: Mathematical Physics (math-ph)
subject: FOS: Physical sciences

description: We compute analytically the all-loop level critical exponents for a massless thermal Lorentz-violating O($N$) self-interacting $λϕ^{4}$ scalar field theory. For that, we evaluate, firstly explicitly up to next-to-leading order and later in a proof by induction up to any loop level, the respective $β$-function and anomalous dimensions in a theory renormalized in the massless BPHZ method, where a reduced set of Feynman diagrams to be calculated is needed. We investigate the effect of the Lorentz violation in the outcome for the critical exponents and present the corresponding mathematical explanation and physical interpretation.
description: 15 pages, 15 figures
(3983) John Boyle
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
DESCRIPTION OF 2 NEW ARACHNOIDEA SPECIES (BRYOZOA, CTENOSTOMIDA) COLLECTED DURING THE KAIKO-I OCEANOGRAPHIC CRUISE (JAPAN)
Creators: name: Dhondt, J. L.
name: Mawatari, S. F.
Publisher: name: Station Biologique de Roscoff (SBR)
Year: 1987

Abstract
title: An Update on the Prostate Imaging Reporting and Data System (PI-RADS) and use of Multi-Parametric Magnetic Resonance Imaging in Non-Invasive Assessment of Prostate Cancer
Creators: name: Bullen, Paul
Publisher: name: The Royal Australian and New Zealand College of Radiologists
Year: 2017
Subjects: subject: Urinary Tract / Bladder
subject: MR-Diffusion/Perfusion
subject: Computer Applications-Detection, diagnosis
subject: Cancer

description: Learning objectives Background Imaging findings OR Procedure details Conclusion Personal information References
description: Learning objectives: The learning objectives for the following exhibit are to outline the use of the PI-RADS Version 2 guidelines in using multi-parametric magnetic resonance imaging (mpMRI) in improving the detection, localization, characterization and risk...
Overview of the software architecture and data flow for the J-PET tomography device
Creators: name: Krzemien, W.
name: Alfs, D.
name: Bialas, P.
name: Czerwinski, E.
name: Gajos, A.
name: Glowacz, B.
name: Jasinska, B.
name: Kaminska, D.
name: Korcyl, G.
name: Kowalski, P.
name: Kozik, T.
name: Kubicz, E.
name: Niedzwiecki, Sz.
name: Pawlik-Niedzwiecka, M.
name: Raczynski, L.
name: Rudy, Z.
name: Silarski, M.
name: Strzelecki, A.
name: Wieczorek, A.
name: Wislicki, W.
name: Zielinski, M.
name: Moskal, P.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Instrumentation and Detectors (physics.ins-det)
subject: Medical Physics (physics.med-ph)
subject: FOS: Physical sciences

description: Modern TOF-PET scanner systems require high-speed computing resources for efficient data processing, monitoring and image reconstruction. In this article we present the data flow and software architecture for the novel TOF-PET scanner developed by the J-PET collaboration. We discuss the data acquisition system, reconstruction framework and image reconstruction software. Also, the concept of computing outside hospitals in the remote centers such as Świerk Computing Centre in Poland is presented.
description: 8 pages, 2 figures
(3984) Delilah Dooley
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
title: Immunocompromised patients with metastatic cutaneous nodal squamous cell carcinoma of the head and neck: Poor outcome is unrelated to the index lesion
Creators: name: Lam, Johnson
Publisher: name: The Royal Australian and New Zealand College of Radiologists
Year: 2017
Subjects: subject: Head and neck
subject: Lymph nodes
subject: Metastases

description: Purpose Methods and materials Results Conclusion Personal information References
description: Purpose: Australia has the highest rate of cutaneous squamous cell carcinoma (cSCC) in the world and subsequently the highest rate of patients developing metastatic cutaneous nodal head and neck SCC (HNSCC). [1] Immunocompromised patients with metastatic...
Additional file 1 of First national-scale evaluation of temephos resistance in Aedes aegypti in Peru
Creators: name: Palomino, Miriam
name: Pinto, Jesus
name: Yañez, Pamela
name: Cornelio, Anali
name: Dias, Luciana
name: Amorim, Quesia
name: Martins, Ademir Jesus
name: Lenhart, Audrey
name: Lima, Jose Bento Pereira
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Ecology
subject: FOS: Biological sciences
subject: Cancer
subject: Hematology
subject: Plant Biology

description: Additional file 1: Table S1. Number of cases of dengue according to departments and geographic regions, Peru 2015–2021.
title: Factory-sealed double-glazing units
Creators: name: Solvason, K. R.
name: Wilson, A. G.
Publisher: name: National Research Council of Canada. Division of Building Research
Year: 1963
Subjects: subject: Moisture performance
valueUri: urn:ircsubjects:IRC0392
subject: Performance hygrométrique
valueUri: urn:ircsubjects:IRC0392
subject: sealed glazing units
subject: double vitre scellées
subject: surface condensation
subject: condensation superficielle
subject: breakage
subject: rupture (fracture)
subject: windows
subject: fenêtre

description: Interior surface temperatures at the edges of sealed double- glazing units are lower than those of many conventional double window arrangements, and the building humidities that can be carried without excessive condensation on these surfaces are correspondingly lower. They must be sealed initially with dry air between the panes, and in spite of the stresses imposed by service conditions must retain the seal over their required service life. Units can fail by either breakage or condensation and the scumming that results from excessive moisture gain. On the other hand, sealed glazing units have advantages in the elimination of storm sash, reduction of surfaces that need cleaning to two, and prevention of condensation between panes. These may often outweigh the potential problems in their use.
Lysyl Hydroxylase 3 Glucosylates Galactosylhydroxylysine Residues in Type I Collagen in Osteoblast Culture
Creators: name: Perdivara, Irina
name: Tomer, Kenneth B.
name: Yamauchi, Mitsuo
name: Yokoyama, Megumi
name: Sricholpech, Marnisa
name: Nagaoka, Hideaki
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: Lysyl hydroxylase 3 (LH3), encoded by Plod3, is the multifunctional collagen-modifying enzyme possessing LH, hydroxylysine galactosyltransferase (GT), and galactosylhydroxylysine-glucosyltransferase (GGT) activities. Although an alteration in type I collagen glycosylation has been implicated in several osteogenic disorders, the role of LH3 in bone physiology has never been investigated. To elucidate the function of LH3 in bone type I collagen modifications, we used a short hairpin RNA technology in a mouse osteoblastic cell line, MC3T3-E1; generated single cell-derived clones stably suppressing LH3 (short hairpin (Sh) clones); and characterized the phenotype. Plod3 expression and the LH3 protein levels in the Sh clones were significantly suppressed when compared with the controls, MC3T3-E1, and the clone transfected with an empty vector. In comparison with controls, type I collagen synthesized by Sh clones (Sh collagen) showed a significant decrease in the extent of glucosylgalactosylhydroxylysine with a concomitant increase of galactosylhydroxylysine, whereas the total number of hydroxylysine residues was essentially unchanged. In an in vitro fibrillogenesis assay, Sh collagen showed accelerated fibrillogenesis compared with the controls. In addition, when recombinant LH3-V5/His protein was generated in 293 cells and subjected to GGT/GT activity assay, it showed GGT but not GT activity against denatured type I collagen. The results from this study clearly indicate that the major function of LH3 in osteoblasts is to glucosylate galactosylhydroxylysine residues in type I collagen and that an impairment of this LH3 function significantly affects type I collagen fibrillogenesis.
(3979) Grace McFarlane
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Are trends in SST, surface Chlorophyll-a, primary production and wind stress similar or different over the decadal scale in the south-eastern Black Sea?
Creators: name: AGIRBAS, Ertugrul
name: FEYZIOGLU, Ali Muzaffer
name: AYTAN, Ulgen
name: VALENTE, Andre
name: YILDIZ, Ilknur
Publisher: name: Station Biologique de Roscoff (SBR)
Year: 2015

description: A 10-year time series of in-situ sea surface temperature (SST), chlorophyll-a concentration and wind stress from 2002 to 2011 was used to examine temporal changes in the South-Eastern Black Sea, together with primary production rates (PP) calculated empirically. Satellite-derived SST was used to support the in-situ SST, and showed a good agreement. The yearly averaged trend for in-situ and satellite-derived winter SST revealed a statistically significant warming over the last decade in the region of interest. Statistically significant correlation between winter NAO and winter averaged SST suggested that winter SST in the South-Eastern Black Sea is influenced by NAO climatic trends. A consistent decrease in wind stress was observed throughout the time series. Inverse relationship between winter averages of SST and wind stress suggested that SST is forced by wind speed. Chl-a and PP fluctuations during the study period revealed that the system is dominated by nanoplankton with some additional contribution from larger species. We concluded that SST in the South- Eastern Black Sea has increased over the decade, whereas the trend in Chl-a and PP rates were not straightforward. Hence, these conditions with respect to other parameters will need to be considered in future studies for the Black Sea ecosystem.
title: A summary of PI-RADS version 2 reporting for prostate carcinoma
Creators: name: Rajadurai, Suraindra
Publisher: name: The Royal Australian and New Zealand College of Radiologists
Year: 2017
Subjects: subject: MR-Functional imaging
subject: MR-Diffusion/Perfusion
subject: MR
subject: Pelvis
subject: Oncology
subject: Genital / Reproductive system male
subject: Diagnostic procedure
subject: Cancer

description: Learning objectives Background Imaging findings OR Procedure details Conclusion Personal information References
description: Learning objectives: This exhibit will briefly review the presentation, diagnosis and early management of prostate cancer and the role of MRI. Subsequently it will review the Prostate Imaging - Reporting and Data System (PI-RADS) v2 protocol for standardised...
Spatial distribution and abundance of intertidal benthic macrofauna in the Kneiss Islands (Gulf of Gabès, Tunisia)
Creators: name: MOSBAHI, Nawfel
name: BOUDAYA, Lobna
name: DAUVIN, Jean-Claude
name: NEIFAR, Lassad
Publisher: name: Station Biologique de Roscoff (SBR)
Year: 2015

description: The Kneiss islands, located in the Gulf of Gabès (Southeastern Tunisia), represent a site of international interest in terms of their ornithological diversity (Important Bird Area, SPAMI and Ramsar Site) and are heavily exploited for clams and fish resources. In an effort to assess the ecological status of the intertidal ecosystem, and to facilitate management and conservation of the resources, an ecological survey of the macrobenthic component was carried out from January to March 2012. This study involved identifying the benthic fauna and assessing the spatial distribution on the southern unperturbed Zostera noltei mudflats by human activities. A total of 102 macrobenthos taxa is identified at 19 stations with a predominance of crustaceans (37%), polychaetes (27%) and molluscs (23%). The AMBI index shows that the area has a good ecological status. Cluster Analysis and Multidimensional scaling identify three benthic assemblages on the basis of their faunal similarity in relation to sediment types and organic matter contents.
Mechanical Regulation of Glycogen Synthase Kinase 3β (GSK3β) in Mesenchymal Stem Cells Is Dependent on Akt Protein Serine 473 Phosphorylation via mTORC2 Protein
Creators: name: Styner, Maya
name: Case, Natasha
name: Galior, Kornelia
name: Rubin, Janet
name: Thomas, Jacob
name: Xie, Zhihui
name: Sen, Buer
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: Mechanical signals can inactivate glycogen synthase kinase 3β (GSK3β), resulting in stabilization of β-catenin. This signaling cascade is necessary for the inhibition of adipogenesis in mesenchymal stem cells (MSC) that is produced by a daily strain regimen. We investigated whether Akt is the mechanically activated kinase responsible for phosphorylation and inactivation of GSK3β in MSC. Mechanical strain (2% magnitude, 0.17 Hz) induced phosphorylation of Akt at Ser-473 and Thr-308 in parallel with phosphorylation of GSK3β at Ser-9. Inhibiting Akt (Akt1/2 kinase inhibitor treatment or Akt knockdown) prevented strain-induced phosphorylation of GSK3β at Ser-9. Inhibition of PI3K prevented Thr-308 phosphorylation, but strain-induced Ser-473 phosphorylation was measurable and induced phosphorylation of GSK3β, suggesting that Ser-473 phosphorylation is sufficient for the downstream mechanoresponse. As Rictor/mTORC2 (mammalian target of rapamycin complex 2) is known to transduce phosphorylation of Akt at Ser-473 by insulin, we investigated whether it contributes to strain-induced Ser-473 phosphorylation. Phosphorylation of Ser-473 by both mechanical and insulin treatment in MSC was prevented by the mTOR inhibitor KU0063794. When mTORC2 was blocked, mechanical GSK3β inactivation was prevented, whereas insulin inhibition of GSK3β was still measured in the absence of Ser-473 phosphorylation, presumably through phosphorylation of Akt at Thr-308. In sum, mechanical input initiates a signaling cascade that is uniquely dependent on mTORC2 activation and phosphorylation of Akt at Ser-473, an effect sufficient to cause inactivation of GSK3β. Thus, mechanical regulation of GSK3β downstream of Akt is dependent on phosphorylation of Akt at Ser-473 in a manner distinct from that of growth factors. As such, Akt reveals itself to be a pleiotropic signaling molecule whose downstream targets are differentially regulated depending upon the nature of the activating input.
SOME OBSERVATIONS ON THE OCCURRENCE AND VERTICAL-DISTRIBUTION OF MITES (ARACHNIDA ACARI) AND OTHER EPIFAUNAL ASSOCIATES OF INTERTIDAL BARNACLES ON 2 CONTRASTED ROCKY SHORES IN NORTH WALES
Creators: name: Demurguia, A. M.
name: Seed, R.
Publisher: name: Station Biologique de Roscoff (SBR)
Year: 1987

Abstract
title: Salvage bevacizumab at symptomatic progression of refractory glioblastoma provides a clinically relevant improvement in survival duration.
Creators: name: Cuncannon, Moire
Publisher: name: The Royal Australian and New Zealand College of Radiologists
Year: 2017
Subjects: subject: CNS
subject: MR
subject: Radiation therapy / Oncology
subject: Outcomes

description: Purpose Methods and materials Results Conclusion Personal information References
description: Purpose: Glioblastoma multiforme (GBM) is the most common and most malignant primary brain tumour in adults [1,2] . These tumours are highly angiogenic with elevated levels of vascular endothelial growth factor (VEGF) [3] . Patients with GBM have poor...
miR-155 Inhibits Expression of the MEF2A Protein to Repress Skeletal Muscle Differentiation
Creators: name: Tatsuguchi, Mariko
name: Wang, Da Zhi
name: Pu, William T.
name: Callis, Thomas E.
name: Seok, Hee Young
name: He, Aibin
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: microRNAs (miRNAs) are 21–23-nucleotide non-coding RNAs. It has become more and more evident that this class of small RNAs plays critical roles in the regulation of gene expression at the post-transcriptional level. MEF2A is a member of the MEF2 (myogenic enhancer factor 2) family of transcription factors. Prior report showed that the 3′-untranslated region (3′-UTR) of the Mef2A gene mediated its repression; however, the molecular mechanism underlying this intriguing observation was unknown. Here, we report that MEF2A is repressed by miRNAs. We identify miR-155 as one of the primary miRNAs that significantly represses the expression of MEF2A. We show that knockdown of the Mef2A gene by siRNA impairs myoblast differentiation. Similarly, overexpression of miR-155 leads to the repression of endogenous MEF2A expression and the inhibition of myoblast differentiation. Most importantly, reintroduction of MEF2A in miR-155 overexpressed myoblasts was able to partially rescue the miR-155-induced myoblast differentiation defect. Our data therefore establish miR-155 as an important regulator of MEF2A expression and uncover its function in muscle gene expression and myogenic differentiation.
TOPoS: II. On the bimodality of carbon abundance in CEMP stars. Implications on the early chemical evolution of galaxies
Creators: name: Bonifacio, P.
name: Caffau, E.
name: Spite, M.
name: Limongi, M.
name: Chieffi, A.
name: Klessen, R. S.
name: François, P.
name: Molaro, P.
name: Ludwig, H. G.
name: Zaggia, S.
name: Spite, F.
name: Plez, B.
name: Cayrel, R.
name: Christlieb, N.
name: Clark, P. C.
name: Glover, S. C. O.
name: Hammer, F.
name: Koch, A.
name: Monaco, L.
name: Sbordone, L.
name: Steffen, M.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Astrophysics of Galaxies (astro-ph.GA)
subject: FOS: Physical sciences

description: In the course of the TOPoS (Turn Off Primordial Stars) survey, aimed at discovering the lowest metallicity stars, we have found several carbon-enhanced metal-poor (CEMP) stars. We here present our analysis of six CEMP stars. Calcium and carbon are the only elements that can be measured in all six stars. The range is -5.0<=[Ca/H]< -2.1 and 7.12<=A(C)<=8.65. For star SDSS J1742+2531 we were able to detect three FeI lines from which we deduced [Fe/H]=-4.80, from four CaII lines we derived [Ca/H]=-4.56, and from synthesis of the G-band we derived A(C)=7.26. For SDSS J1035+0641 we were not able to detect any iron lines, yet we could place a robust (3sigma) upper limit of [Fe/H]< -5.0 and measure the Ca abundance, with [Ca/H]=-5.0, and carbon, A(C)=6.90. No lithium is detected in the spectrum of SDSS J1742+2531 or SDSS J1035+0641, which implies a robust upper limit of A(Li)<1.8 for both stars. Our measured carbon abundances confirm the bimodal distribution of carbon in CEMP stars, identifying a high-carbon band and a low-carbon band. We propose an interpretation of this bimodality according to which the stars on the high-carbon band are the result of mass transfer from an AGB companion, while the stars on the low-carbon band are genuine fossil records of a gas cloud that has also been enriched by a faint supernova (SN) providing carbon and the lighter elements. (Abridged)
description: to be published on A&A
Modulation of Prothrombinase Assembly and Activity by Phosphatidylethanolamine
Creators: name: Lentz, Barry R.
name: Quinn-Allen, Mary Ann
name: Liang, Xiaoe
name: Majumder, Rinku
name: Kane, William H.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: Constituents of platelet membranes regulate the activity of the prothrombinase complex. We demonstrate that membranes containing phosphatidylcholine and phosphatidylethanolamine (PE) bind factor Va with high affinity (Kd = ∼10 nm) in the absence of phosphatidylserine (PS). These membranes support formation of a 60–70% functional prothrombinase complex at saturating factor Va concentrations. Although reduced interfacial packing does contribute to factor Va binding in the absence of PS, it does not correlate with the enhanced activity of the Xa-Va complex assembled on PE-containing membranes. Instead, specific protein-PE interactions appear to contribute to the effects of PE. In support of this, soluble C6PE binds to recombinant factor Va2 (Kd = ∼6.5 μm) and to factor Xa (Kd = ∼91 μm). C6PE and C6PS binding sites of factor Xa are specific, distinct, and linked, because binding of one lipid enhances the binding and activity effects of the other. C6PE triggers assembly (Kdapp = ∼40 nm) of a partially active prothrombinase complex between factor Xa and factor Va2, compared with Kdapp for C6PS ∼2 nm. These findings provide new insights into the possible synergistic roles of platelet PE and PS in regulating thrombin formation, particularly when exposed membrane PS may be limiting.
Molecular Insights into DNA Polymerase Deterrents for Ribonucleotide Insertion
Creators: name: Cavanaugh, Nisha A.
name: Wilson, Samuel H.
name: Pedersen, Lee G.
name: Perera, Lalith
name: Beard, William A.
name: Batra, Vinod K.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: DNA polymerases can misinsert ribonucleotides that lead to genomic instability. DNA polymerase β discourages ribonucleotide insertion with the backbone carbonyl of Tyr-271; alanine substitution of Tyr-271, but not Phe-272, resulted in a >10-fold loss in discrimination. The Y271A mutant also inserted ribonucleotides more efficiently than wild type on a variety of ribonucleoside (rNMP)-containing DNA substrates. Substituting Mn2+ for Mg2+ decreased sugar discrimination for both wild-type and mutant enzymes primarily by increasing the affinity for rCTP. This facilitated crystallization of ternary substrate complexes of both the wild-type and Y271A mutant enzymes. Crystallographic structures of Y271A- and wild type-substrate complexes indicated that rCTP is well accommodated in the active site but that O2′ of rCTP and the carbonyl oxygen of Tyr-271 or Ala-271 are unusually close (∼2.5 and 2.6 Å, respectively). Structure-based modeling indicates that the local energetic cost of positioning these closely spaced oxygens is ∼2.2 kcal/mol for the wild-type enzyme. Because the side chain of Tyr-271 also hydrogen bonds with the primer terminus, loss of this interaction affects its catalytic positioning. Our results support a model where DNA polymerase β utilizes two strategies, steric and geometric, with a single protein residue to deter ribonucleotide insertion.
A new algorithm for contact angle estimation in molecular dynamics simulations
Creators: name: YD, Sumith
name: Maroo, Shalabh C.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Soft Condensed Matter (cond-mat.soft)
subject: Computational Engineering, Finance, and Science (cs.CE)
subject: FOS: Physical sciences
subject: FOS: Computer and information sciences

description: It is important to study contact angle of a liquid on a solid surface to understand its wetting properties, capillarity and surface interaction energy. While performing transient molecular dynamics (MD) simulations it requires calculating the time evolution of contact angle. This is a tedious effort to do manually or with image processing algorithms. In this work we propose a new algorithm to estimate contact angle from MD simulations directly and in a computationally efficient way. This algorithm segregates the droplet molecules from the vapor molecules using Mahalanobis distance (MND) technique. Then the density is smeared onto a 2D grid using 4th order B-spline interpolation function. The vapor liquid interface data is estimated from the grid using density filtering. With the interface data a circle is fitted using Landau method. The equation of this circle is solved for obtaining the contact angle. This procedure is repeated by rotating the droplet about the vertical axis. We have applied this algorithm to a number of studies (different potentials and thermostat methods) which involves the MD simulation of water.
(3982) Elisabeth Lamont
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Mutation of the 5′-Untranslated Region Stem-Loop Structure Inhibits α1(I) Collagen Expression in Vivo
Creators: name: Latour, Anne M.
name: Brenner, David A.
name: Stefanovic, Branko
name: Marzluff, William F.
name: Parsons, Christopher J.
name: Aoyama, Tomonori
name: Rippe, Richard A.
name: Seki, Ekihiro
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: Type I collagen is a heterotrimeric extracellular matrix protein consisting of two α1(I) chains and one α2(I) chain. During liver fibrosis, activated hepatic stellate cells (HSCs) are the major source of the type I collagen that accumulates in the damaged tissue. Expression of α1(I) and α2(I) collagen mRNA is increased 60-fold compared with quiescent stellate cells and is due predominantly to post-transcriptional message regulation. Specifically, a stem-loop structure in the 5′-untranslated region of α1(I) collagen mRNA may regulate mRNA expression in activated HSCs through its interaction with stem-loop binding proteins. The stem-loop may also be necessary for efficient production and folding of the type I collagen heterotrimer. To assess the role of the stem-loop in type I collagen expression in vivo, we generated a knock-in mouse harboring a mutation that abolished the stem-loop structure. Heterozygous and homozygous knock-in mice exhibited a normal phenotype. However, steady-state levels of α1(I) collagen mRNA decreased significantly in homozygous mutant MEFs as well as HSCs; intracellular and secreted type I collagen protein levels also decreased. Homozygous mutant mice developed less liver fibrosis. These results confirm an important role of the 5′ stem-loop in regulating type I collagen mRNA and protein expression and provide a mouse model for further study of collagen-associated diseases.
title: Blunt Cerebrovascular Injury Screening protocol at Auckland City Hospital: is it worthwhile?
Creators: name: Ormiston, William
Publisher: name: The Royal Australian and New Zealand College of Radiologists
Year: 2017
Subjects: subject: MR-Angiography
subject: CT-Angiography
subject: Vascular
subject: Neuroradiology brain
subject: Catheter arteriography
subject: Diagnostic procedure
subject: Trauma
subject: Dissection

description: Purpose Methods and materials Results Conclusion Personal information References
description: Purpose: The relationship between blunt trauma and damage to the cerebrovascular circulation of the head and neck has been widely reported [1]. The benefits of both screening the population at risk of these injuries, and the subsequent treatment of...