Additional file 1 of ADP-ribosyl transferase activity and gamma radiation cytotoxicity of Pseudomonas aeruginosa exotoxin A
Creators: name: Morgan, Radwa N.
name: Saleh, Sarra E.
name: Aboshanab, Khaled M.
name: Farrag, Hala A.
Publisher: name: figshare
Year: 2021
Subjects: subject: Biochemistry
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Science Policy
subject: 20199 Astronomical and Space Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Additional file 1. Figure S1 Bootstrap tree for P. aeruginosa isolate (22) 16S ribosomal RNA sequence against 16S ribosomal RNA sequences retrieved from NCBI GenBank. Figure S2 Bootstrap tree for P. aeruginosa isolate (16) 16S ribosomal RNA sequence against 16S ribosomal RNA sequences retrieved from NCBI GenBank. Figure S3 Bootstrap tree for P. aeruginosa isolate (1) 16S ribosomal RNA sequence against 16S ribosomal RNA sequences retrieved from NCBI GenBank. Figure S4 Bootstrap tree for P. aeruginosa isolate (39) 16S ribosomal RNA sequence against 16S ribosomal RNA sequences retrieved from NCBI GenBank. Figure S5 UV and FTIR absorbance spectra for lyophilized powder nitrobenzylidine aminoguanidine (NBAG). Figure S6 Mass spectra for lyophilized powder, nitrobenzylidine aminoguanidine (NBAG). Figure S7 13C-NMR spectrum for lyophilized powder, nitrobenzylidine aminoguanidine (NBAG). Figure S8 Agarose Gel electrophoresis for the detection of toxA amplicons. Figure S9 Bootstrap tree for partial sequence of toxA gene (Exotoxin A Partial P. aeruginosa isolate 1) blasted against toxA gene records retrieved from NCBI GenBank. Figure S10 Bootstrap tree for partial sequence of toxA gene (Exotoxin A Partial P. aeruginosa isolate 1) blasted against toxA gene records retrieved from European nucleotide Archives (ENA). Figure S11 Bootstrap tree for translated toxA gene (Exotoxin A (Fragment) OS P. aeruginosa isolate 1) blasted against exotoxin A protein records retrieved from Uniprot Database. Figure S12 Alignment of toxA sequence and deletion in the Adenine base. Figure S13 The absorbance spectra of ADP ribosylated nitrobenzylidine aminoguanidine (NBAG). Figure S14 Reduction in the absorbance maxima post exposure to gamma irradiated exotoxin A protein extract. Figure S15 HPLC chromatogram of P. aeruginosa isolate 1 at 301 nm. Figure S16 HPLC chromatogram of P. aeruginosa isolate 5 at 301 nm. Figure S17 HPLC chromatogram of P. aeruginosa isolate 35 at 301 nm. Figure S18 HPLC chromatogram of PA 39 isolate at 301 nm. Figure S19 HPLC chromatogram of P. aeruginosa isolate 22 irradiated at 5Gy at 301 nm. Figure S20 HPLC chromatogram of P. aeruginosa isolate 22 irradiated at 15Gy at 301 nm. Figure S21 HPLC chromatogram of P. aeruginosa isolate 22 irradiated at 10Gy at 301 nm. Figure S22 HPLC chromatogram of P. aeruginosa isolate 16 irradiated at 15Gy at 301 nm. Figure S23 HPLC chromatogram of P. aeruginosa isolate 16 irradiated at 10Gy at 301 nm. Table S1 Computed Pairwise distance between P. aeruginosa isolate 22, 16S ribosomal RNA and 16S ribosomal RNA sequences retrieved from NCBI GenBank. Table S2 Computed Pairwise distance between P. aeruginosa isolate 16, 16S ribosomal RNA and 16S ribosomal RNA sequences retrieved from NCBI GenBank. Table S3 Computed Pairwise distance between P. aeruginosa isolate 1, 16S ribosomal RNA and 16S ribosomal RNA sequences retrieved from NCBI GenBank. Table S4 Computed Pairwise distance between P. aeruginosa isolate 39, 16S ribosomal RNA and 16S ribosomal RNA sequences retrieved from NCBI GenBank. Table S5 Computed Pairwise distance between Exotoxin A partial sequence P. aeruginosa isolate 1 and toxA gene sequences retrieved from NCBI GenBank. Table S6 Computed Pairwise distance between Exotoxin A partial sequence P. aeruginosa isolate 1 and toxA gene sequences retrieved from European nucleotide archive (ENA) and NCBI GenBank. Table S7 Absorbance maxima and expected amount of ADP-ribosylated NBAG formed by P. aeruginosa exotoxin A protein extract. Table S8 Absorbance maxima post exposure to gamma irradiated exotoxin A extract and percent reduction in ADP-ribosylated NBAG formed. Table S9 Percent cytotoxicity and viability for exotoxin A protein extract on cultured Hep2 cells. Table S10 Percent cell viability and increase in cellular viability post exposure to irradiated P. aeruginosa exotoxin A protein extract.