Trichoderma globoides W. T. Qin & W. Y. Zhuang HMAS 2017, sp. nov.
Creators: name: Qin, Wen-Tao
name: Zhuang, Wen-Ying
Publisher: name: Zenodo
Year: 2017
Subjects: Biodiversity
Taxonomy
Fungi
Ascomycota
Sordariomycetes
Hypocreales
Hypocreaceae
Trichoderma
Trichoderma globoides

description: Trichoderma globoides W.T. Qin & W.Y. Zhuang, sp. nov. FIGURE 4   
 Etymology:— The specific epithet refers to the globose conidia.   
 MycoBank: MB 816631   
 Type: — CHINA. HUBEI: Shennongjia, Shennongyuan, 2250 m, on rotten twigs, 13 Sep 2014, W. T. Qin, Z.Q. Zeng, H.D. Zheng & K. Chen 10648 (holotype HMAS 248747).   
On CMD after 72 h 50–52 mm; mycelium covering the plate after 5 d at 25 C. Colony typically zonate, aerial hyphae densely disposed with a well-defined margin. Conidiation noted after 2 d, starting in short minute shrubs, spreading, growing to tufts or pustules, green. Conidiophores regular tree-like and side branches emerging from the main axis mostly at right angles, often paired or in verticils, also unpaired with a single whorl of phialides at upper levels, straight or slightly curved. Phialides in whorls of 2–4(–5), lageniform to subulate, uncommonly ampulliform, (6.8–)7.2–11.3(– 12.4) × (1.5–)1.7–3.1 μm, l/ w 2.8 –5.2(–5.5), 1.2–1.7 μm wide at the base (n = 50). Conidia green, globose, subglobose to ellipsoidal, smooth, 3–4.5(–5.2) × (2.5–)3–4 μm, l/ w 1.0 –1.2(–1.6) (n = 50). Chlamydospores uncommon, terminal or intercalary, globose or ellipsoidal, 6–9(–10) × (5.2–)5.5–7.2(–8.2) μm, l/w (1.0–)1.2–1.3(–1.5) (n = 50). No odor; no diffusing pigment observed.  
On PDA after 72 h 45–47 mm; mycelium covering the plate after 5 d at 25 C. Colony dense, covered by thick, loose, white tomentum aerial hyphae several mm high (later collapsing). Whitish tufts formed after 2 d around the plug, irregularly disposed, turning green after 3–4 d, also conidiation on aerial hyphae turning green.  
On SNA after 72 h 35–37 mm; mycelium covering the plate after 7 d at 25 C. Colony zonate, similar to CMD; hyphae loosely disposed, more curly. Conidiation noted after 2 d on the aerial hyphae. No odor; no diffusing pigment noted.  
 Notes: —In the phylogenetic tree, T. globoides is associated with T. alni, T. corneum and T. italicum (MPBP / BIPP = 90%/100%) of the Harzianum clade (Figure 1). Trichoderma corneum differs in its verticillium-like conidiophores, larger phialides [8–24 × 1.5–3.0 μm], and narrower conidia [2.0–5.0 × 1.6–2.8 μm] (Doi 1972, Chaverri & Samuels 2003). Trichoderma italicum is characterized by production of the bright yellow pustules in culture and smaller conidia [(2.7–)3.0–3.5(–4.3) × (2.3–)2.5–2.8(–3.2) μm] (Jaklitsch & Voglmayr 2015). Trichoderma alni is slow-growing [CMD 35–40 mm, PDA 27–32 mm, SNA 30–32 mm], and forms conidiophores with simple branches and longer phialides [(8–)9–15(–18) × (2.2–)2.5–3.0(–3.5) μm] compared with T. globoides (Jaklitsch et al. 2008, Jaklitsch 2009).
description: Published as part of Qin, Wen-Tao & Zhuang, Wen-Ying, 2017, Seven new species of Trichoderma (Hypocreales) in the Harzianum and Strictipile clades, pp. 121-139 in Phytotaxa 305 (3) on pages 129-130, DOI: 10.11646/phytotaxa.305.3.1, http://zenodo.org/record/13694923