The Arabidopsis class II sirtuin is a lysine deacetylase and interacts with mitochondrial energy metabolism
Creators: name: König, Ann-Christine
name: Hartl, Markus
name: Pham, Phuong Anh
name: Laxa, Miriam
name: Boersema, Paul J.
name: Orwat, Anne
name: Kalitventseva, Ievgeniia
name: Plöchinger, Magdalena
name: Braun, Hans-Peter
name: Leister, Dario
name: Mann, Matthias
name: Wachter, Andreas
name: Fernie, Alisdair R.
name: Finkemeier, Iris
Publisher: name: Rockville, Md. : American Society of Plant Physiologists
Year: 2014
Subjects: subject: adenosine diphosphate
subject: adenosine triphosphate
subject: Arabidopsis protein
subject: carbon
subject: histone deacetylase
subject: lysine
subject: messenger RNA
subject: mitochondrial protein
subject: nicotinamide adenine dinucleotide
subject: sirtuin
subject: SRT2 protein, Arabidopsis
subject: acetylation
subject: amino acid sequence
subject: Arabidopsis
subject: article
subject: cell respiration
subject: chemistry
subject: energy metabolism
subject: enzyme specificity
subject: enzymology
subject: gene inactivation
subject: genetics
subject: metabolism
subject: mitochondrial membrane
subject: mitochondrion
subject: molecular genetics
subject: nonsense mediated mRNA decay
subject: phenotype
subject: protein binding
subject: protein transport
subject: RNA splicing
subject: Acetylation
subject: Adenosine Diphosphate
subject: Adenosine Triphosphate
subject: Amino Acid Sequence
subject: Arabidopsis Proteins
subject: Carbon Isotopes
subject: Cell Respiration
subject: Energy Metabolism
subject: Gene Knockout Techniques
subject: Histone Deacetylases
subject: Lysine
subject: Mitochondria
subject: Mitochondrial Membranes
subject: Mitochondrial Proteins
subject: Molecular Sequence Data
subject: NAD
subject: Nonsense Mediated mRNA Decay
subject: Phenotype
subject: Protein Binding
subject: Protein Transport
subject: RNA Splicing
subject: RNA, Messenger
subject: Sirtuins
subject: Substrate Specificity
subject: Dewey Decimal Classification::500 | Naturwissenschaften::580 | Pflanzen (Botanik)

description: The posttranslational regulation of proteins by lysine (Lys) acetylation has recently emerged to occur not only on histones, but also on organellar proteins in plants and animals. In particular, the catalytic activities of metabolic enzymes have been shown to be regulated by Lys acetylation. The Arabidopsis (Arabidopsis thaliana) genome encodes two predicted sirtuin-type Lys deacetylases, of which only Silent Information Regulator2 homolog (SRT2) contains a predicted presequence for mitochondrial targeting. Here, we have investigated the function of SRT2 in Arabidopsis. We demonstrate that SRT2 functions as a Lys deacetylase in vitro and in vivo. We show that SRT2 resides predominantly at the inner mitochondrial membrane and interacts with a small number of protein complexes mainly involved in energy metabolism and metabolite transport. Several of these protein complexes, such as the ATP synthase and the ATP/ADP carriers, show an increase in Lys acetylation in srt2 loss-of-function mutants. The srt2 plants display no growth phenotype but rather a metabolic phenotype with altered levels in sugars, amino acids, and ADP contents. Furthermore, coupling of respiration to ATP synthesis is decreased in these lines, while the ADP uptake into mitochondria is significantly increased. Our results indicate that SRT2 is important in fine-tuning mitochondrial energy metabolism.