title: ÐÐ½ÑÐµÑÐ½Ð°Ð»Ð¸Ð·Ð°ÑÐ¸Ñ ÑÐ¸Ð½ÑÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð°Ð½Ð°Ð»Ð¾Ð³Ð¾Ð² Ð¿ÑÐ¾Ð»Ð¸Ð½-Ð±Ð¾Ð³Ð°ÑÑÑ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð² Ð² Ð¾Ð¿ÑÑ Ð¾Ð»ÐµÐ²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸ in vitro
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Internalization of synthetic analogues of proline-rich peptides in tumor cells in vitro
Creators: name: ÐÐ¾Ð²Ð±ÑÑ, ÐÐ»ÐµÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: Ð¿ÑÐ¾Ð»Ð¸Ð½-Ð±Ð¾Ð³Ð°ÑÑÐµ Ð¿ÐµÐ¿ÑÐ¸Ð´Ñ
Ð±Ð°ÐºÑÐµÐ½ÐµÑÐ¸Ð½Ñ
Ð¿ÑÐ¾Ð½Ð¸ÐºÐ°ÑÑÐ¸Ðµ Ð² ÐºÐ»ÐµÑÐºÑ Ð¿ÐµÐ¿ÑÐ¸Ð´Ñ
Ð°Ð½ÑÐ¸Ð¼Ð¸ÐºÑÐ¾Ð±Ð½ÑÐµ Ð¿ÐµÐ¿ÑÐ¸Ð´Ñ
Ð°Ð´ÑÐµÑÐ½Ð°Ñ Ð´Ð¾ÑÑÐ°Ð²ÐºÐ°
ÑÐ¸ÑÐ¾ÑÐ¾ÐºÑÐ¸ÑÐµÑÐºÐ¾Ðµ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ðµ
bodipy fl
proline-rich peptides
bactenecins
cell-penetrating peptides
antimicrobial peptides
targeted delivery
cytotoxic action

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÐ¸ Ðº Ð¸Ð½ÑÐµÑÐ½Ð°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð² Ð¾Ð¿ÑÑÐ¾Ð»ÐµÐ²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸ ÑÑÐ´Ð° ÑÐ¸Ð½ÑÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð°Ð½Ð°Ð»Ð¾Ð³Ð¾Ð² Ð¿ÑÐ¾Ð»Ð¸Ð½-Ð±Ð¾Ð³Ð°ÑÑÑ Ð°Ð½ÑÐ¸Ð¼Ð¸ÐºÑÐ¾Ð±Ð½ÑÑ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð² (ÐÐÐ) Ð»ÐµÐ¹ÐºÐ¾ÑÐ¸ÑÐ¾Ð² Ð´Ð¾Ð¼Ð°ÑÐ½ÐµÐ¹ ÐºÐ¾Ð·Ñ Capra hircus â Ð±Ð°ÐºÑÐµÐ½ÐµÑÐ¸Ð½Ð¾Ð², Ð¸ Ð¾ÑÐµÐ½ÐºÐ¸ Ð¸Ñ ÑÐ²Ð¾Ð¹ÑÑÐ² ÐºÐ°Ðº Ð¿ÑÐ¾Ð½Ð¸ÐºÐ°ÑÑÐ¸Ñ Ð² ÐºÐ»ÐµÑÐºÐ¸ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð² (CPP). ÐÐ»Ñ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾ÑÑÐ¸ Ð´ÐµÑÐµÐºÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð² Ð²Ð¾ Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¼ Ð¿ÑÐ¾ÑÑÑÐ°Ð½ÑÑÐ²Ðµ Ð±ÑÐ»Ð° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð¿ÑÐ¾ÑÐµÐ´ÑÑÐ° Ð¸Ñ ÐºÐ¾Ð½ÑÑÐ³Ð°ÑÐ¸Ð¸ Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½ÑÐ¼ Ð·Ð¾Ð½Ð´Ð¾Ð¼ BODIPY FL. Ð­ÑÐ¾ Ð¿Ð¾Ð·Ð²Ð¾Ð»Ð¸Ð»Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÑ Ð¸Ð½ÑÐµÑÐ½Ð°Ð»Ð¸Ð·Ð°ÑÐ¸Ñ Ð¼ÐµÑÐµÐ½ÑÑ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð² Ð½Ð° Ð¼Ð¾Ð´ÐµÐ»Ð¸ in vitro Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¿ÑÐ¾ÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐ¸ÑÐ¾ÑÐ»ÑÐ¾ÑÐ¸Ð¼ÐµÑÑÐ¸Ð¸, Ð° ÑÐ°ÐºÐ¶Ðµ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð¹, Ð² ÑÐ¾Ð¼ ÑÐ¸ÑÐ»Ðµ ÐºÐ¾Ð½ÑÐ¾ÐºÐ°Ð»ÑÐ½Ð¾Ð¹, Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾-Ð¼ÐµÑÐµÐ½Ð½ÑÐµ Ð¾ÑÐ¸ÑÐµÐ½Ð½ÑÐµ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÑ ÑÐ¸Ð½ÑÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð°Ð½Ð°Ð»Ð¾Ð³Ð¾Ð² Ð¿ÑÐ¾Ð»Ð¸Ð½-Ð±Ð¾Ð³Ð°ÑÑÑ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð², Ð¸ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð¾Ð½Ð¸ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ Ð¿ÑÐ¾Ð½Ð¸ÐºÐ°ÑÑ Ð² Ð¼Ð°Ð»Ð¸Ð³Ð½Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐµ ÐºÐ»ÐµÑÐºÐ¸, Ð½Ðµ Ð¾ÐºÐ°Ð·ÑÐ²Ð°Ñ Ð¿ÑÐ¸ ÑÑÐ¾Ð¼ ÑÐ¸ÑÐ¾ÑÐ¾ÐºÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ. Ð­ÑÐ¾ Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÐµÑ ÑÐ°ÑÑÐ¼Ð°ÑÑÐ¸Ð²Ð°ÑÑ Ð´Ð°Ð½Ð½ÑÐµ Ð¿ÑÐ¾Ð»Ð¸Ð½-Ð±Ð¾Ð³Ð°ÑÑÐµ Ð¿ÐµÐ¿ÑÐ¸Ð´Ñ Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð¾ÑÐ½Ð¾Ð²Ñ Ð´Ð»Ñ ÑÐ¾Ð·Ð´Ð°Ð½Ð¸Ñ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð² Ð°Ð´ÑÐµÑÐ½Ð¾Ð¹ Ð´Ð¾ÑÑÐ°Ð²ÐºÐ¸ ÑÐµÑÐ°Ð¿ÐµÐ²ÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð°Ð³ÐµÐ½ÑÐ¾Ð² Ð² Ð¾Ð¿ÑÑÐ¾Ð»ÐµÐ²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸.
description: The given work is devoted to the study of an ability of various synthetic analogues of proline-rich antimicrobial peptides (AMP) of leukocytes of the domestic goat Capra hircus â bactenecins to internalize into tumor cells. Their properties as cell-penetrating peptides (CPP) have been explored. To be able to detect the peptides in the cells, we conjugated them with BODIPY FL fluorescent probe. This allowed us to study the process of internalization of the labeled peptides in vitro using flow cytofluorometry, fluorescence microscopy and including the confocal one. As a result of the work, we obtained fluorescently labeled purified preparations of synthetic analogues of proline-rich peptides that efficiently penetrated cancer cells without exerting cytotoxic effects. Taking into account these findings, we can consider these proline-rich peptides as the base for targeted drug delivery to tumor cells.