MOESM1 of Development of a new method for collecting hemolymph and measuring phenoloxidase activity in Tribolium castaneum
Creators: name: Tabunoki, Hiroko
name: Dittmer, Neal
name: Gorman, Maureen
name: Kanost, Michael
Publisher: name: figshare
Year: 2019
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Molecular Biology
subject: Neuroscience
subject: Physiology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: FOS: Chemical sciences
subject: Ecology
subject: Immunology
subject: FOS: Clinical medicine
subject: FOS: Clinical medicine
subject: Developmental Biology
subject: Inorganic Chemistry
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: FOS: Health sciences

description: Additional file 1: Figure S1. Identifying the sex of pupal and adult T. castaneum. The structure of the genital papillae is markedly different between female and male pupae. The pupae were sexed by examining the structure of the genital papillae (on the last abdominal segment), which are markedly different between female and male pupae (indicated with black arrow). For adults, sex was determined by the presence (male) or absence (female) of a patch of bristles (“sex patch”; indicated by white arrow) on the femur of the prothoracic legs. Figure S2. Observation of the reaction curve in experimental condition 1. The absorbance (A470) was monitored, and calculated as mO.D./min (ΔA470 = 0.001/min), and plotted on the graph. Control, no added bacteria; M. luteus indicates plasma samples treated with M. luteus prior to PO assay. A; larva control, B; larva M. luteus, C; Adult control, D; Adult M. luteus. Figure S3. Observation of the reaction curve in Experimental condition 2. The absorbance (A470) was monitored, and calculated as mO.D./min (ΔA470 = 0.001/min), and plotted on the graph. Control, no added bacteria; M. luteus indicates plasma samples treated with M. luteus prior to PO assay. A; larva control, B; larva M. luteus, C; Adult control, D; Adult M. luteus. Figure S4. Observation of the reaction curve in Experimental condition 3. The absorbance (A470) was monitored, and calculated as mO.D./min (ΔA470 = 0.001/min), and plotted on the graph. Control, no added bacteria; M. luteus indicates plasma samples treated with M. luteus prior to PO assay. A; larva control, B; larva M. luteus, C; Adult control, D; Adult M. luteus. Figure S5. Observation of the reaction curve in Experimental condition 4. The absorbance (A470) was monitored, and calculated as mO.D./min (ΔA470 = 0.001/min), and plotted on the graph. Control, no added bacteria; M. luteus indicates plasma samples treated with M. luteus prior to PO assay. A; larva control, B; larva M. luteus, C; Adult control, D; Adult M. luteus. Figure S6. Observation of the reaction curve in Experimental condition 5. The absorbance (A470) was monitored, and calculated as mO.D./min (ΔA470 = 0.001/min), and plotted on the graph. Control, no added bacteria; M. luteus indicates plasma samples treated with M. luteus prior to PO assay. A; larva control, B; larva M. luteus, C; Adult control, D; Adult M. luteus. Figure S7. Observation of the reaction curve in Experimental condition 6. The absorbance (A470) was monitored, and calculated as mO.D./min (ΔA470 = 0.001/min), and plotted on the graph. Control, no added bacteria; M. luteus indicates plasma samples treated with M. luteus prior to PO assay. A; larva control, B; larva M. luteus, C; Pupa-male control, D; Pupa-male M. luteus, E; Pupa-female control, F; Pupa-female M. luteus, G; Adult-male control, H; Adult-male M. luteus, I; Adult-female control, J; Adult-female M. luteus. Table S1. Plasma collected from different developmental stages (n = 30). Table S2. Assay buffer combinations tested for measuring phenoloxidase (PO) activity. Table S3. Phenoloxidase activity in experimental combination 1 to 5.