Femtosecond laser fabricated spike structures for selective control of cellular behavior
Creators: name: Schlie, Sabrina
name: Fadeeva, Elena
name: Koch, Jürgen
name: Ngezahayo, Anaclet
name: Chichkov, Boris
Publisher: name: London : SAGE Publications Ltd.
Year: 2010
Subjects: subject: biomedicine
subject: cellular adhesion
subject: femtosecond laser
subject: microstructures
subject: surface topography
subject: water contact angle
subject: Cellular adhesion
subject: Cellular behaviors
subject: Functional surfaces
subject: Human fibroblast
subject: Material effect
subject: Neuroblastoma cells
subject: Process parameters
subject: Selective control
subject: Silicone elastomers
subject: Soft material
subject: Surface contact
subject: Surface microstructures
subject: Wetting property
subject: Adhesion
subject: Cell culture
subject: Contact angle
subject: Fibroblasts
subject: Growth kinetics
subject: Microstructure
subject: Pulsed laser applications
subject: Silicones
subject: Surface topography
subject: Ultrashort pulses
subject: Surfaces
subject: biomaterial
subject: elastomer
subject: nanomaterial
subject: silicon
subject: article
subject: cell adhesion
subject: cell proliferation
subject: cellular, subcellular and molecular biological phenomena and functions
subject: chemistry
subject: cytology
subject: DNA damage
subject: fibroblast
subject: human
subject: laser
subject: metabolism
subject: mutagen testing
subject: neuroblastoma
subject: surface property
subject: time
subject: tumor cell line
subject: ultrastructure
subject: Biocompatible Materials
subject: Cell Adhesion
subject: Cell Line, Tumor
subject: Cell Physiological Processes
subject: Cell Proliferation
subject: DNA Damage
subject: Elastomers
subject: Humans
subject: Lasers
subject: Mutagenicity Tests
subject: Nanostructures
subject: Neuroblastoma
subject: Silicon
subject: Surface Properties
subject: Time Factors
subject: Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit

description: In this study we investigate the potential of femtosecond laser generated micrometer sized spike structures as functional surfaces for selective cell controlling. The spike dimensions as well as the average spike to spike distance can be easily tuned by varying the process parameters. Moreover, negative replications in soft materials such as silicone elastomer can be produced. This allows tailoring of wetting properties of the spike structures and their negative replicas representing a reduced surface contact area. Furthermore, we investigated material effects on cellular behavior. By comparing human fibroblasts and SH-SY5Y neuroblastoma cells we found that the influence of the material was cell specific. The cells not only changed their morphology, but also the cell growth was affected. Whereas, neuroblastoma cells proliferated at the same rate on the spike structures as on the control surfaces, the proliferation of fibroblasts was reduced by the spike structures. These effects can result from the cell specific adhesion patterns as shown in this work. These findings show a possibility to design defined surface microstructures, which could control cellular behavior in a cell specific manner.