cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Novel target identification & characterisation of key cell motility regulators in lung cancer metastasis
Creators: name: Munro, Catriona
Publisher: name: Imperial College London
Year: 2014

description: Lung cancer is the most common cancer killer worldwide. One of the major reasons for failing to cure this malignancy is the high rate of metastasis. Hence, understanding the mechanisms by which these cells metastasise is required to improve patients’ survival. Using the non-small cell lung cancer cell line A549 we optimised a 3D Invasion assay to enable a robotised high-throughput siRNA screen of large gene libraries. Despite thorough optimisation a pilot screen for the Phosphatome highlighted multiple issues with our set-up. Further optimisation work was conducted to improve the resolving power of the assay. However, as no clear explanation for the poor reproducibility could be uncovered, work began on a potential modulator of cell motility identified during a previous motility screen of the kinome. Microtubule affinity regulating kinases 4 (MARK4) is one of four members of the MARK family. A previously performed kinome siRNA screen for modulators of cell migration revealed that depletion of MARK4 reduced A549 cell migration. We validated this observation and additionally found that MARK4 silencing inhibited cell invasion through 3D collagen matrices. MARK4 depletion markedly changed cell structure, as exemplified by an increase tubulin network area. Follow-on experiments revealed an altered speed of microtubule polymerisation in MARK4-silenced cells. We observed that MARK4 downregulation promoted resistance to the chemotherapeutic agents Paciltaxel and Cisplatin, an effect potentially linked to a reduced proliferation in MARK4-silenced cells. MARK4 overexpression in NSCLC cells increased cell motility but did not impact the cell area or resistance to chemotherapy. A targeted siRNA cell motility screen of a selection of proposed MARK4 interacting proteins enabled us to connect MARK4 with Protein Phosphatase 2A and GSK3. Although further investigation is required into the signalling pathways upstream and downstream of MARK4, this work identified novel functions for this kinase and highlighted potential mechanisms underlying its effects.
Barriers to testing for Human Immunodeficiency Virus infection in the United Kingdom
Creators: name: Elmahdi, Rahma
Publisher: name: Imperial College London
Year: 2014

description: In recent years there has been an increased focus on biomedical interventions as a means of Human immunodeficiency Virus (HIV) prevention and the use of antiretroviral therapy (ART) has been a particularly successful tool in prevention efforts, with evidence for treatment in reducing HIV transmission. This is dependent on several factors including the early identification of those infected with HIV. In this thesis I will explore current challenges to testing for HIV in the UK by systematically reviewing current national levels of testing and investigating demographic characteristics associated with timing of diagnosis, testing practices and routes to diagnosis among those recently diagnosed with HIV in West London in order to identify barriers to increased and repeat testing for HIV in the UK. My findings show that guideline recommended testing levels are poor in most clinical settings and this is reflected in overall low HIV test coverage in the UK. HIV diagnosis at an earlier point in infection remains significantly associated with men who have sex with men (MSM) and White ethnicity and both patient and provider barriers act as ongoing challenges in earlier identification of HIV in all groups. Current testing practices are not enough to achieve equitable access to early diagnosis for HIV. Testing practices of clinicians, along with system challenges play an important role in HIV testing and changing these may be the most effective method of increasing earlier identification of HIV positive individuals in the UK.
Modelling antibody responses to malaria blood stage infections: A novel method to estimate malaria transmission intensity from serological data
Creators: name: Pothin, Emilie
Publisher: name: Imperial College London
Year: 2013

description: Infection with the Plasmodium falciparum malaria parasite results in an immune response which includes the production of antibodies against the blood-stage of infection. In recent years there has been an increase in the use of serological data to monitor malaria transmission intensity. Traditionally, EIR and parasite prevalence were the preferred tools for measuring malaria transmission intensity. Serology has been shown to be particularly useful in areas of low endemicity where traditional measures (EIR and parasite prevalence) are problematic. Transmission intensity in this case is usually described by the seroconversion rate obtained from fitting a catalytic model to age-stratified serological responses. The aim of my thesis was to better utilise the continuous measurements of antibody responses provided by serology studies to obtain improved estimates of transmission intensity. To do this, I developed a series of biologically motivated models to mimic the acquisition and decay in blood-stage antibody responses. In the first part of the thesis, I developed a discrete model as a direct extension of the catalytic model and fitted this to cross-sectional data from several sites in Cambodia to obtain an estimate of the exposure rate. In the second part of the thesis a series of continuous density models were developed to mimic antibody acquisition and loss for P. falciparum infections. These models were fitted to both the Cambodian data and separately validated by fitting to data from Tanzanian villages at a wider range of transmission intensities. In the final section I applied and extended the model to encompass a wider range of endemic transmission in Somalia, Bioko Island, Gambia and Uganda in order to assess the robustness of the method. My results show that estimates of the exposure rate obtained by fitting the density model are highly correlated with classic malariometric indices and that a key advantage of this approach is the increased precision in the estimates compared to estimates of the seroconversion rate, especially in areas of low transmission. This method could therefore be a useful alternative framework for quantifying transmission intensity which makes more complete use of serological data and shows potentials for detecting heterogeneity in malaria exposure.
title: Die „anticholinerge Last” als Demenz-Risikofaktor: Ergebnisse einer Untersuchung an 936 älteren, ambulant urologischen Patienten mit Überaktiver Blase
Creators: name: Wiedemann, A.
name: Ivchenko, A.
name: Heppner, H.-J.
Publisher: name: German Medical Science GMS Publishing House
Year: 2020
Subjects: subject: 610 Medical sciences; Medicine

description: Einleitung: Bewegungsmangel, Diabetes mellitus, Rauchen, Übergewicht, Depression, arterielle Hypertonie und die chronische medikamentöse Therapie mit Anticholinergika (Gray SL, 2015) gelten als Risikofaktoren einer Demenz. Gerade eine solche anticholinerge Therapie stellt jedoch die Basis [zum vollständigen Text gelangen Sie über die oben angegebene URL]
description: 46. Gemeinsame Tagung der Bayerischen Urologenvereinigung und der Österreichischen Gesellschaft für Urologie und Andrologie
The effect of Toxoplasma gondii on host behaviour: Studies on evolution and mechanisms of action
Creators: name: Kaushik, Maya
Publisher: name: Imperial College London
Year: 2013

description: Toxoplasma gondii affects a range of intermediate and secondary host species, including humans, with cats (Felidae) being the definitive host. The mechanism of action of T. gondii in its natural rat host can provide profound insights as to evolutionary trajectory of parasite manipulation. I used a range of non-invasive behavioural and physiological assays to assess the impact of infection on different aspects of innate rat behaviour. One key unique focus was an examination of the potential effect(s) of novel genetically modified parasite lines that overexpress tyrosine hydroxylase (TgTH) in comparison to wildtype T. gondii. The aim here was to test the hypothesis that TgTH, through its subsequent impact on neuromodulator levels, plays a significant mechanistic role in host behaviour modification. Consistent with previous studies, there was a clear effect of T. gondii on host behaviour, with effects seen in generalised anxiety and feline response tests. However contrary to predictions, there was little or no significant differences between wildtype and TgTH overexpressor infected rats in feline response tests, implying that TgTH does not play a major role in feline or olfactory related predation response. The implications of the role of TgTH in generalised anxiety and activity levels were more mixed. This suggests that other mechanisms of action, probably multiple, are involved. This work increases our understanding of the potential mechanisms this parasite has evolved to attain specific manipulation of the rodent intermediate host to increase transmission success to the feline definitive host. The results thus have both evolutionary implications for parasite-altered behaviour in intermediate host species, and applied implications in terms of the potential impact on health and behaviour of all infected hosts, including humans.
Reversing DNA methylation by heterokaryon-mediated reprogramming
Creators: name: Piccolo, Francesco M.
Publisher: name: Imperial College London
Year: 2012

description: Genomic imprinting directs the allele‐specific expression of a subset of loci according to their parental origin. Monoallelic expression of these genes is regulated by imprinting control regions (ICRs) and is established in the embryonic germ line through differential DNA methylation. Differentiated cells can be reprogrammed to pluripotency by several strategies including the ectopic expression of specific ‘inducers’ and by transfer of nuclei into enucleated eggs. Cellular fusion of somatic cells with a pluripotent stem cell partner can also lead to dominant pluripotent reprogramming. Although ES cells (derived from the inner cell mass) and embryonic germ cells (EG, derived from primordial germ cells) can both reprogram, EG cells are unique in being able to erase genomic imprints from the somatic partner. In order to characterize the earliest events in successful reprogramming, as well as EG‐specific DNA demethylation, I generated experimental heterokaryons between B‐lymphocytes and mouse stem cell lines. I showed that ES cells that lack Polycomb Repressor Complex 2 (PRC2) failed to reprogram B cells and were unable to induce two early events that characterise successful B cell reprogramming; a global redistribution of HP1α and an increased serine 10 phosphorylation. In the second part of my study I confirmed that EG cells were able to induce DNA demethylation at several ICR in B cells following fusion. I present evidence that this reprogramming of the somatic genome requires Tet1 and Tet2 and is accomplished through a two‐step process involving both DNA synthesis and conversion of 5methylcytosine into 5hydroxymethylcytosine.
CCWI2017: Conference Booklet
Creators: name: Collins, Richard
Publisher: name: The University of Sheffield
Year: 2017
Subjects: subject: 90599 Civil Engineering not elsewhere classified
subject: FOS: Civil engineering

description: CCWI2017: 15th International Computing & Control for the Water Industry Conference5th - 7th September 2017 at the Diamond, University of Sheffield

CCWI2017: Conference Booklet
Teamarbeit und Kommunikation
Creators: name: Kovacevic, Toni
Publisher: name: Fachhochschule Nordwestschweiz FHNW
Year: 2018

description: Die nachfolgende Untersuchung befasst sich mit den Fragen, wie Teams des Bereichs Prävention 1 der Aidshilfe beider Basel untereinander kommunizieren und welche Einflussgrössen auf die Kommunikationsart herrschen. Die qualitativ durchgeführte Untersuchung operationalisierte den Begriff der Teamarbeit anhand einer fünf relevanten Einflussfaktoren – Kommunikation – indem die einzelnen Teammitglieder ihr Interaktionsverhalten in einem Workshop sowie einem halbstrukturierten Leitfadeninterview darlegten. Es konnte aufgezeigt werden, dass die Teamarbeit primär auf virtuelle Art geschieht und die Kommunikationskanäle dementsprechend gewählt werden. Nebst dem meistgewählten Medium E-Mail werden aber vorzugsweise Gespräche auf bilateraler, wie auch auf Teamebene in Form von Meetings geführt, da alle Befragten darin die grösste Klarheit und Effizient im Informationsaustausch sehen. Bemängelt wird aber die Möglichkeit nach Face-to- Face-Interkation, welche laut Aussagen der Befragten das Teamgefühl stärke, was wiederum in einem grösseren Verständnis für die Mitglieder und für die Arbeitsprozesse münden würde.
title: Was können die Maschinen? – Praktischer Nutzen von Big Data und KI in der Medizin
Creators: name: Piotrowski, A.
Publisher: name: German Medical Science GMS Publishing House
Year: 2020
Subjects: subject: 610 Medical sciences; Medicine

description: Mit zunehmender Rechen- und Speicherkapazität hält in der Medizin fast unaufhaltsam die Anwendung von Big Data und Künstlicher Intelligenz Einzug. Wo kann diese sinnvoll eingesetzt werden? Haupteinsatzgebiete sind bisher vor allem in der Radiologie und Pathologie zu finden.[zum vollständigen Text gelangen Sie über die oben angegebene URL]
description: 46. Gemeinsame Tagung der Bayerischen Urologenvereinigung und der Österreichischen Gesellschaft für Urologie und Andrologie
ОСТРЫЙ ЛАРИНГОТРАХЕИТ У ДЕТЕЙ
Creators: name: Ибодуллаева, Ш.Ю.
Publisher: name: Zenodo
Year: 2024

description: Изучение особенностей современного течения острого ларинготрахеита у детей, а также анализ основных принципов купирования  острого ларинготрахеита у детей на современном этапе.
Mobil-flexibles Arbeiten in Bürolandschaften–Chancen und Risiken
Creators: name: Schulze, Hartmut
Publisher: name: Fachhochschule Nordwestschweiz FHNW
Year: 2020
Subjects: subject: Gestaltung von Büroräumen
subject: mobil-flexibles Arbeiten
subject: Partizipation
subject: 100 - Philosophie und Psychologie
subject: 300 - Sozialwissenschaften
subject: 600 - Technik, Medizin, angewandte Wissenschaften

description: - Mobil-flexible Arbeitsweisen nehmen zu, Arbeit findet zunehmend an verschiedenen Orten statt (mobil-flexible Arbeitsweise) - Präsenz wird immer mehr zum knappen Gut («qualitytime») - Angemessener «Mix» aus Home Office und Arbeit vor Ort hängt ab von u.a. Art der Aufgabe, Ausstattung Home Office, Bedürfnissen der Mitarbeitenden - Es stellen sich «neue» Anforderungen für Teamarbeit und sozialen Austausch (u.a. Co-working) - Die Rolle der Führungskraft verändert sich in Richtung Coach, als «Enabler», als Unterstützer - Training und Weiterbildung für Führungskräfte und Mitarbeitende ist wichtig - Die Beteiligung aller Akteure als Voraussetzung, um die zukünftig relevanten Arbeits-und Lehr-/Lernwelten «passend» zu den Anforderungen gestalten zu können
Evaluating the impact of financial incentives on inequalities in smoking cessation in primary care
Creators: name: Hamilton, Fiona Louise
Publisher: name: Imperial College London
Year: 2013

description: Background: Smoking cessation interventions are underprovided in primary care. This thesis examines the impact of financial incentives on the provision of smoking cessation interventions, and inequalities in provision, in primary care. Methods: • Systematic review of financial incentives for smoking cessation in healthcare. • Cross sectional study using general practice data from Wandsworth, London, using logistic regression to examine associations between ethnicity and disease group with ascertainment of smoking status and provision of cessation advice following the introduction of the UK’s Quality and Outcomes Framework (QOF). • Before-and-after studies using general practice data from Hammersmith & Fulham, London, looking at the impact of a local financial incentive scheme (QOF+) on smoking outcomes for patients without smoking-related diseases (primary prevention), and antenatal patients, using logistic regression to examine inequalities. Results: Introduction of financial incentives was associated with increased recording of smoking status and advice to smokers, most evident for patients with smoking-related diseases compared with patients without smoking-related diseases, for whom there were much smaller incentives for recording smoking status and none for offering stop smoking advice. However, when specific incentives were provided for primary prevention large improvements in smoking outcomes were seen. The youngest and oldest groups of patients were less likely to be asked about smoking. White British patients were more likely to smoke than other ethnic groups, except Black Caribbean men with depression, 62% of whom smoked. Smoking advice was provided relatively equitably, but Black Caribbean men with depression were less likely to receive advice than White British men with depression (59% vs 81%). Disparities in smoking outcomes with respect to age and ethnicity persisted after the financial incentives were introduced. Conclusions: Introduction of financial incentives was associated with increases in recording smoking status and largely equitable provision of cessation advice, but variations in smoking outcomes between groups persisted. Extending financial incentives to include recording of ethnicity and rewarding quit rates may further improve smoking outcomes in primary care.
Development of LC-MS method and myoblast differentiation model for studying the mechanism of DNA demethylation
Creators: name: Turp, Aleksandra
Publisher: name: Imperial College London
Year: 2013

description: DNA methylation is an epigenetic modification important in many cellular processes such as maintenance of genome stability, establishment and maintenance of imprinting, transposon silencing, chromatin remodelling and control of gene expression. It is therefore important to understand how this modification is established and erased. We set out to develop a sensitive, liquid–chromatography mass–spectrometry method to measure global levels of DNA methylation (5mdC), as well as hydroxymethylation (5hmdC), a potential intermediate of DNA demethylation. With the new Agilent 6490 QQQ LC–MS we were able to detect as little as 50 amol of 5mdC and 5hmdC. We used this method to quantify levels of DNA methylation from DNA extracted from only 100 cells, allowing us to compare DNA methylation levels in early zygote development. Given that the evidence for DNA demethylation in early zygotes comes from methods using either antibody staining or bisulfite sequencing, this is the first direct demonstration that global DNA demethylation occurs in zygotes. Myoblast differentiation is a well-known model of DNA demethylation, where locus-specific DNA demethylation at the promoters and enhancer elements of myogenic regulatory factors, such as myogenin and MyoD, induce muscle specification and differentiation. It has been proposed, however, that the DNA demethylation process involves a large proportion of the genome and that it occurs in the absence of replication, indicating an active process. Therefore, in the second part of this work I set out to further investigate the global scale of epigenetic events associated with myoblast differentiation. Whilst some of the myoblast differentiation experiments showed a marked wave of DNA demethylation (up to 51%) others did not show any changes in DNA methylation level, showing that myoblast differentiation and DNA demethylation are not co-dependent. Addition of a DNA demethylating agent, 5-Aza-3’-deoxycytidine, to the growth medium of differentiating myoblast enhanced the differentiation process. On the other hand, inhibition of Poly (ADP- ribose) polymerase 1 activity, which has been shown to be mechanistically involved in DNA demethylation, inhibited the differentiation process. My work documents the kinetics of 5- v methylcytosine abundance during the myoblast differentiation together with the expression dynamics of factors previously linked to the DNA demethylation process.
title: Schichtarbeit und Hypogonadismus
Creators: name: Eisenmenger, M.
name: Endler, G.
Publisher: name: German Medical Science GMS Publishing House
Year: 2020
Subjects: subject: 610 Medical sciences; Medicine

description: Einleitung: Ca. 20% aller Arbeitskräfte in den Industriestaaten sind Schichtarbeiter, in Österreich 2017 rund 744.000 Personen. Da der Mensch eine tageszyklische Spezies ist, löst die Schichtarbeit Störungen der zirkadianen Rhythmik aus. Durch den gestörten Wach-Schlaf-Rhythmus[zum vollständigen Text gelangen Sie über die oben angegebene URL]
description: 46. Gemeinsame Tagung der Bayerischen Urologenvereinigung und der Österreichischen Gesellschaft für Urologie und Andrologie
Diagnostic strategies in pelvic floor disorders
Creators: name: Ramage, Lisa
Publisher: name: Imperial College London
Year: 2018

description: Introduction: Management of pelvic floor disorders is undertaken within tertiary-level referral centres. However, the majority of patients do not require surgery. This thesis sets out to identify who is most likely to require surgery versus who could be initially managed in a primary care setting. Methods: Patient reported outcome measure (PROM) tools and investigatory data collated prospectively was explored alongside maximal treatment interventions. Additionally, MR-defecography (MRD) results were analysed in a cohort of patients with symptoms of obstructed defecation (ODS). Specific patient populations forming a large proportion of those requiring surgery were explored separately: patients with obstetric anal sphincter injury (OASI), and patients who had undergone surgery for ultralow rectal cancer with either a handsewn or stapled anastomosis. Results: 276 patients with faecal incontinence (FI) and 129 patients with ODS were explored. 17.39% of those with FI and 20.93% with ODS ultimately required surgery. The majority of patients required only Biofeedback. The FI domain of the BBUSQ and several domains of the SF-36 were fair predictors of the need for surgery in FI sufferers. On anorectal manometry, only the squeeze endurance increment was predictive of the need for surgery in patients with FI. There was no demonstrable relationship between the PROMS or MRD and the requirement for surgery in ODS sufferers. Patients with a missed OASI had worse function at initial follow-up. At early follow-up no differences were found between different OASI grades. Patients with a handsewn coloanal anastomosis had worse function than those with a stapled anastomosis. Conclusion: There needs to be a move towards more community-based care in patients presenting with pelvic floor dysfunction to ease the burden on tertiary-level services with development of an integrated care pathway which streamlines referrals based on the likelihood for the need for more invasive treatment strategies.
Charles mills on deracializing liberalism
Creators: name: Fleischacker, Samuel
Publisher: name: University of Illinois at Chicago
Year: 2022
Subjects: Uncategorized

description: This collection of Charles Mills’ writings includes his famous “White Ignorance” and “Kant’s Untermenschen,” along with his most extensive engagement with the writings of John Rawls. Fleischacker’s review endorses and expands Mills’ critique of what Rawls calls “ideal theory,” while disputing Mills’ characterization of Kant’s moral theory as intrinsically racist. It proposes a different way of understanding how Kant and other philosophers have been able to maintain egalitarian principles while still being racist.
Investigating reward systems in the human midbrain with fMRI
Creators: name: Limbrick-Oldfield, Eve Helen
Publisher: name: Imperial College London
Year: 2012

description: Localising activity in the human midbrain with conventional functional MRI (fMRI) is challenging because the midbrain nuclei are small and located in an area that is prone to physiological artefacts. In the first section of this thesis I present a replicable and automated method to improve the detection and localisation of midbrain fMRI signals. I designed a visual fMRI task that was predicted would activate the superior colliculi bilaterally. A novel anatomical registration pathway was used to optimise the localisation of the small midbrain nuclei in stereotactic space. This pathway was compared to conventional registration pathways, and was shown to significantly improve midbrain registration. To reduce the physiological artefacts in the functional data, I estimated and removed structured noise using a modified version of a previously described physiological noise model (PNM). Whereas a conventional analysis revealed only unilateral colliculi activity, the PNM analysis revealed the predicted bilateral activity. I then used these methods to investigate the role of the midbrain dopaminergic system in reward. Midbrain dopamine neurons play central roles in positive reward prediction errors (PEs). Non-human studies now indicate that dopamine neurons also code for aversive, punishing events, and may code negative PEs. However, our understanding of how such events are coded in the human dopaminergic midbrain is limited, and has been hindered by the technical challenge of using fMRI in this region. Here I show that during a financial reward and punishment task the ventral tegmental area (VTA) codes for both positive and negative PEs. The fMRI data from the midbrain contained a significant amount of physiological noise, but once I applied the PNM responses were also observed in the substantia nigra pars compacta (SNc), with negative PE signals extending more laterally. These findings indicate that both the human VTA and SNc code for both positive and negative PEs.
CCWI2017: Figshare Repository
Creators: name: Collins, Richard
Publisher: name: The University of Sheffield
Year: 2017
Subjects: subject: 90599 Civil Engineering not elsewhere classified
subject: FOS: Civil engineering

description: This is the Figshare repository for the papers for the Computing and Control for the Water Industry (CCWI) conference held at Sheffield 5th to 7th.

The papers included in the repository will be remain to be available after the conference and all have an individual d.o.i. and will be completely discoverable by common and academic search engines.

The papers should appear ordered as they are in the Conference booklet, ordered by theme. You can also reorder the papers as you desire or search by author or title. In addition you will find the items that relate to the conference themes these include a list of papers for each theme and a direct link to each paper via the d.o.i.

We hope you enjoy the conference and find it a useful and engaging 3 days.

Regards

Local Organising Committee
Marketing Automation: Den Erfolg auf Autopilot setzen
Creators: name: Dalla Vecchia, Martina
Publisher: name: Fachhochschule Nordwestschweiz FHNW
Year: 2017
Subjects: subject: Detailkenntnisse
subject: Informationen
subject: Kundenservice
subject: 330 - Wirtschaft

description: Digitale Transformation im Marketing hat einen Namen: Marketing Automation. Die Grundidee ist, Prozesse so zu designen, dass sie zwar automatisiert ablaufen, aber auf jeden einzelnen Kunden zugeschnitten sind. In diesem Sinne ist Marketing Automation eine Form von Mass Customization für den Verkaufserfolg.
Wie Führungskräfte in komplexen Projekten durch Coaching unterstützt werden können - Am Beispiel der Projektallianz
Creators: name: Merz, Tobias
Publisher: name: Fachhochschule Nordwestschweiz FHNW
Year: 2018
Subjects: Coaching
komplexe Projekte
Projektallianz
Führung
Projektleitung
Handling Komplexität
Projektleitungscoaching
Führungskräftecoaching
Anforderung Coach und Projektleitung

description: Komplexe Projekte sind heutzutage weit verbreitet. Es wird daher untersucht, wie Führungskräfte in komplexen Projekten durch Coaching unterstützt werden können. Dabei dient die Projektallianz beispielhaft als Organisationsmethode für komplexe Projekte. In einer Dokumentenanalyse wurde einerseits erhoben, worin die Unterstützungsbedürfnisse von Projektleitenden in ebensolchen komplexen Projekten bestehen, zum anderen, in welcher Art und Weise dieser Bedarf jeweils gedeckt werden kann. In 13 Experteninterviews (d.h. Coaches, Projektleitende und Schlüsselpersonen aus dem Kompetenzzentrum Projektallianz der SBB) wurden weitere Anhaltspunkte zu Bedürfnissen hinsichtlich Unterstützung und deren Deckung erhoben. Die Resultate deuten darauf hin, dass die Führungskräfte bei unterschiedlichen Themen Unterstützung benötigen. Daraus werden acht Handlungsempfehlungen zu Themen erarbeitet, welche die Führungskräfte in komplexen Projekten unterstützen können: komplexe Projekte; Führungskräftecoaching; Führungskräfteweiterbildung; Kommunikationsschulung; Kultur der Projektallianz; Projektmanagement- und Arbeitstechnik-Auffrischung; rund ums Führungskräftecoaching sowie Zusammenarbeit und Team.
title: Endogene vasoaktive Peptide in der Kontrolle des Corpus cavernosum penis: Hat das Vasopressin eine physiologische Relevanz?
Creators: name: Ückert, S.
name: Becker, A.
name: Bannowsky, A.
name: Tsikas, D.
name: Kuczyk, M.
Publisher: name: German Medical Science GMS Publishing House
Year: 2020
Subjects: subject: 610 Medical sciences; Medicine

description: Allgemeines: Neben den klassischen Transmittern des adrenergen Systems sind auch nicht-adrenerge Substanzen an der Aufrechterhaltung des konstanten Tonus des penilen erektilen Gewebes (Corpus cavernosum = CC) in der Phase der Flakzidität/der Terminierung der Erektion beteiligt. Das Peptidhormon[zum vollständigen Text gelangen Sie über die oben angegebene URL]
description: 46. Gemeinsame Tagung der Bayerischen Urologenvereinigung und der Österreichischen Gesellschaft für Urologie und Andrologie
A global potential energy surface for H2S+(X 4A′′) and quasi-classical trajectory study of the S+(4S) + H2(X1Σ+g) reaction
Creators: name: Y. Z. Song
name: Y. Zhang
name: S. B. Gao
name: Q. T. Meng
name: C. K. Wang
name: M. Y. Ballester
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Biophysics
subject: Biochemistry
subject: 29999 Physical Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: FOS: Biological sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Inorganic Chemistry

description: A novel global potential energy surface for H2S+(X 4A″) based on accurate ab initio calculations is presented. Energies are calculated at the multi-reference configuration interaction level with Davidson correction using aug-cc-pVQZ basis set plus core-polarisation high-exponent d functions. A grid of 4552 points is used for the least-square fitting procedure in the frame of a many-body expansion. The topographical features of the new potential energy surface are here discussed in detail. Such a surface is then employed for dynamic studies of the S(4S) + H2(X 1Σ+g) →SH+(X 3Σ−) + H(2S) reaction using the quasi-classical trajectory method. State specific trajectories are calculated, for both ground and ro-vibrationally excited initial states of H2(X 1Σ+g). Corrections to the zero point energy leakage of the classical calculations are also presented. Calculated reaction cross sections and rate constants are here reported and compared with available literature.
One Year Minergie-A—Switzerlands Big Step towards Net ZEB
Creators: name: Hall, Monika
Publisher: name: David Publishing Company
Year: 2013
Subjects: Net zero energy building
net zero energy balance
embodied energy
life cycle energy
Minergie-A

description: The first available label standardizing a zero-balanced type of building is the Swiss Standard Minergie-A. The Standard prescribes an annual net zero primary energy balance for heating, domestic hot water and ventilation. Electricity consumption for appliances and lighting is excluded. Additionally, Minergie-A is the first standard worldwide which includes a requirement in regard to embodied energy. Based on an analysis of 39 Minergie-A buildings, this paper shows that a wide range of different energy concepts and embodied energy strategies are possible in the scope of the label. The basis of all Minergie-A buildings is a well-insulated building envelope. However, the step from the Swiss Standard Minergie-A to a Net ZEB (net zero energy building) standard which includes electricity consumption for appliances and lighting is not a very big one. Increasing the size of the photovoltaic system is sufficient in most cases. Anyway, some of the Minergie-A buildings evaluated are also Net ZEBs. In this paper, it is also shown that the net zero balance during the operational phase of Net ZEBs clearly outweighs the increased embodied energy for additional materials in a life cycle energy analysis.
Additional file 1 of Association of cardiovascular health and epigenetic age acceleration
Creators: name: Pottinger, Tess D.
name: Khan, Sadiya S.
name: Zheng, Yinan
name: Zhang, Wei
name: Tindle, Hilary A.
name: Allison, Matthew
name: Wells, Gretchen
name: Shadyab, Aladdin H.
name: Nassir, Rami
name: Martin, Lisa Warsinger
name: Manson, JoAnn E.
name: Lloyd-Jones, Donald M.
name: Greenland, Philip
name: Baccarelli, Andrea A.
name: Whitsel, Eric A.
name: Hou, Lifang
Publisher: name: figshare
Year: 2021
Subjects: subject: Medicine
subject: Cell Biology
subject: Sociology
subject: FOS: Sociology
subject: Cancer
subject: Mental Health

description: Additional file 1: Supplemental Figures and Tables.
Efficient one-pot three-component method for the synthesis of highly fluorescent coumarin-containing 3,5-disubstituted-2,6-dicyanoaniline derivatives under microwave irradiation
Creators: name: Aydıner, Burcu
name: Yalçın, Ergin
name: Korkmaz, Vildan
name: Seferoğlu, Zeynel
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Biochemistry
subject: 29999 Physical Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: Physiology
subject: FOS: Biological sciences
subject: Pharmacology
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Inorganic Chemistry

description: A simple and concise approach to the synthesis of coumarin-containing highly fluorescent asymmetric/symmetric 3,5-diaryl/heteroaryl-2,6-dicyanoaniline derivatives are reported. The compounds were synthesized through base catalyzed three-component one-pot synthesis reaction of 2-(1-(7-(diethylamino)-2-oxo-2H-chromen-3-yl)ethylidene)malononitrile, aliphatic, aromatic/heteroaromatic aldehydes, and malononitrile in solvent-free reaction medium, catalyzed by piperidine under microwave irradiation method. The reaction gave the novel, highly fluorescent coumarin-containing 3,5-disubstituted-2,6-dicyanoaniline derivatives in good yields at 300 W, 80 °C in 2 min. This method provides several advantages; such as shorter reaction time, environmental friendliness, simple workup procedure, lower energy consumption and, generally, good yields with high purity. In addition, the synthesized compounds are fluorescently active dyes even in day light, except for compounds including nitro substituent, and show maximum absorption wavelengths (λabs.max) in the visible region in DMSO at room temperature. Thermal properties of all the synthesized compounds were also evaluated with thermal gravimetric analysis for usability as optic dye. Optical and thermal screening studies of the compounds showed that the dyes have excellent photophysical and thermal stability properties.
The effect of arachidonic acid on the early and late stages of adipocyte differentiation
Creators: name: Nikolopoulou, Evanthia
Publisher: name: Imperial College London
Year: 2013

description: Dietary fat has been correlated with obesity since it induces the proliferation and differentiation of pre-adipocytes. However it has become clear that the effect of fat on human health depends on the composition and the nature of fatty acids. Arachidonic acid (AA) is a major omega-6 polyunsaturated fatty acid. Its role in adipocyte differentiation is controversial as published data have shown opposing roles of AA in pre-adipocyte differentiation. We investigated the effect of a brief exposure of AA on pre-adipocyte differentiation. After a 24h of AA treatment during pre-adipocyte differentiation, the expression of aP2, a differentiation marker, and Fra-1, an AP-1 transcription factor, was induced. We showed that this effect was PPARγ-dependent since AA treatment was unable to up-regulate aP2 and Fra-1 in PPARγ knockdown cells. Furthermore, we identified that both of these genes were regulated by the AA metabolite, PGF2α, through binding to its cognate GPCR, the FP receptor, initiating a cascade of signalling pathways involving PKC and ERK activation. We also showed that short-term treatment of pre-adipocytes with AA at the beginning of differentiation induced events with a long-lasting inhibitory effect on adipogenesis. Treatment of pre-adipocytes with AA for only 24h, blocked adipogenesis as revealed by significantly reduced expression of adipocyte markers and lipid accumulation after 10 days of differentiation. We demonstrated that Fra-1 mediated the inhibitory effect of AA, as Fra-1 depletion rescued the inhibition of differentiation by AA. We suggest that short-term exposure of pre-adipocytes to AA at the early stages of differentiation has a dual effect. In the early stages, the presence of AA causes a rapid up-regulation in two target genes, the aP2 and Fra-1, in a PPARγ-dependent way. In the later stages of differentiation, when AA is withdrawn, the early up-regulation of Fra-1 results in inhibition of the differentiation program.
title: Langzeitergebnisse nach operativer Behandlung der kongenitalen Penisdeviation mit einer modifizierten Nesbit-Technik
Creators: name: Sokolakis, I.
name: Schönbauer, P.
name: Kübler, H.
name: Gschwend, J.
name: Lahme, S.
name: Hatzichristodoulou, G.
Publisher: name: German Medical Science GMS Publishing House
Year: 2020
Subjects: subject: 610 Medical sciences; Medicine

description: Einleitung: Die kongenitale Penisdeviation (angeborene Penisverkrümmung, CPC) ist eine seltene Erscheinung, bei der die chirurgische Korrektur die Standardtherapie bei betroffenen Patienten ist. Wir berichten über Langzeitergebnisse einer prospektiven Studie, in der die chirurgischen und funktionellen[zum vollständigen Text gelangen Sie über die oben angegebene URL]
description: 46. Gemeinsame Tagung der Bayerischen Urologenvereinigung und der Österreichischen Gesellschaft für Urologie und Andrologie
Signaling through reactive oxygen and nitrogen species is differentially modulated in sunflower seedling root and cotyledon in response to various nitric oxide donors and scavengers*
Creators: name: Singh, Neha
name: Bhatla, Satish C.
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Biophysics
subject: Biochemistry
subject: Space Science
subject: Medicine
subject: Physiology
subject: FOS: Biological sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: Plant Biology

description: Sodium nitroprusside (SNP), diethylenetriamine NONOate (DETA), S-nitroso-n-acetyl-D,L- penicillamine (SNAP), and 4-(p-methoxyphenyl)-1,3,2- Oxathiazolylium-5-olate (CAY) exhibit differential NO releasing ability in aqueous solution and hemoglobin is a more efficient NO quencher than cPTIO in solution. DETA releases 16% more NO compared with SNP in solution. Various NO donors (SNP, DETA, SNAP, and CAY) also bring about a differential but concentration-dependent increase in endogenous NO in seedling cotyledons and roots. Two-day old, dark-grown seedling roots exhibit 95%, 77%, 59% and 45% increase in NO content in presence of each of 500 µM of DETA, SNAP, CAY and SNP, respectively, relative to control. NO accumulation in the tissue system as a response to NO donors is reflected in terms of corresponding peroxynitrite accumulation. Release of cyanide and free iron as byproducts of SNP dissociation in solution limits its usefulness as an NO donor. SNP leads to profuse ROS generation in sunflower seedling roots. Light is not a pre-requisite for NO generation from SNP. Present work also demonstrates the usefulness of hemoglobin over cPTIO as NO scavenger. Hemoglobin brings about increasing NO quenching with its increasing concentration from 2.5 to 10 µM. Greater sensitivity of the root system to the NO donor/scavenger treatments is evident, it being in direct contact with the molecules in the incubation/ growth medium. This differential effect does not seem to be significantly transmitted to the cotyledons (long-distance signaling).
Characterizing cytotoxic and estrogenic activity of Arctic char tissue extracts in primary Arctic char hepatocytes
Creators: name: Petersen, Karina
name: Hultman, Maria T.
name: Bytingsvik, Jenny
name: Harju, Mikael
name: Evenset, Anita
name: Tollefsen, Knut Erik
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Biochemistry
subject: Molecular Biology
subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: FOS: Biological sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Inorganic Chemistry

description: Contaminants from various anthropogenic activities are detected in the Arctic due to long-range atmospheric transport, ocean currents, and living organisms such as migrating fish or seabirds. Although levels of persistent organic pollutants (POPs) in Arctic fish are generally low, local hot spots of contamination were found in freshwater systems such as Lake Ellasjøen at Bjørnøya (Bear Island, Norway). Higher concentrations of organic halogenated compounds (OHC), and higher levels of cytochrome P450 and DNA-double strand breaks were reported in Arctic char (Salvelinus alpinus) from this lake compared to fish from other lakes on Bjørnøya. Although several of the measured contaminants are potential endocrine disrupters, few studies have investigated potential endocrine disruptive effects of the contaminant cocktail in this fish population. The aim of this study was to compare acutely toxic and estrogenic potency of the cocktail of pollutants as evidenced by cytotoxic and/or estrogenic effects in vitro using extracts of Arctic char livers from contaminated Lake Ellasjøen with those from less contaminated Lake Laksvatn at Bjørnøya. This was performed by in situ sampling and contaminant extraction from liver tissue, followed by chemical analysis and in vitro testing of the following contaminated tissue extracts: F1-nonpolar OHC, F2-polar pesticides and metabolites of OHC, and F3-polar OHC. Contaminant levels were highest in extracts from Ellasjøen fish. The F2 and F3 extracts from Lake Laksvatn and Lake Ellasjøen fish reduced in vitro cell viability at a concentration ratio of 0.03–1 relative to tissue concentration in Arctic char. Only the F3 liver extract from Ellasjøen fish increased in vitro vitellogenin protein expression. Although compounds such as estrogenic OH-PCBs were quantified in Ellasjøen F3 extracts, it remains to be determined which compounds were inducing estrogenic effects.
Wie KMU variable Vergütung einsetzen
Creators: name: Lüthi, Erhard
name: Reber, Melanie
Publisher: name: Engeli
Year: 2017
Subjects: subject: Vergütungsmanagement
subject: variable Vergütung
subject: Compensation
subject: 330 - Wirtschaft

description: Erfolgs- und leistungsorientierte Vergütung wird heut auch im Bereich der KMUs vermehrt diskutiert. Die Studie gibt einen Einblick in die Häufigkeit und die Ausgestaltung von variablen Vergütungssystemen von KMUs in der deutschsprachigen Schweiz und untersucht deren wahrgenommenen Auswirkungen auf die Mitarbeitenden.
Midbrain dopaminergic neuron fate specification of pluripotent stem cells
Creators: name: Jaeger, Ines
Publisher: name: Imperial College London
Year: 2012

description: Disorders of dopaminergic neurons in the midbrain are associated with various brain diseases such as Parkinson’s disease (PD), for which there are no effective treatments. Pluripotent stem cells (PSCs) offer a remarkable potential for finding new therapeutic strategies because they are self-renewable and capable to differentiate into all cell types. However, biomedical applications of PSCs require knowledge of the molecular mechanisms directing PSCs into midbrain dopaminergic (mDA) cell fate. During development the generation of mDA neurons is induced by the extrinsic molecules SHH, FGF8 and WNT1 in the caudal region of the Otx2 positive domain, which marks the presumptive fore- and midbrain, and rostrally to the future hindbrain expressing Gbx2. In this study I have further investigated the role of FGF signalling in the generation of mDA neurons. Here, I have used mouse epiblast stem cells (EpiSCs). Based on their developmentally primed pluripotent state, we assumed that they represent a more suitable system when compared with mouse embryonic stem cells. I validated neural differentiation of EpiSCs as an alternative model to study neural development in vitro. I found that inhibition of the FGF/ERK activity at the onset of EpiSC differentiation initiated expression of Wnt and Shh and further induced ventral midbrain progenitor markers, accompanied by suppressing caudalisation as well as forebrain induction. To maintain ventral midbrain progenitor fate, cells required a period of endogeneous FGF/ERK signalling. Subsequent treatment of FGF8 and SHH, which restricts progenitors from adopting alternative fates, led to highly efficient production of authentic mDA neurons. These neurons exhibited functional, neuron-like properties and when implanted into the striatum of mouse PD model strongly restored parkinsonian features without any signs of overgrowth. In conclusion, a temporally controlled modulation of FGF/ERK activity during neural differentiation from PSCs is crucial for reliable and highly efficient generation of functional authentic mDA neurons.
Multi-resolution super learner for voxel-wise classification of prostate cancer using multi-parametric MRI
Creators: name: Jin, Jin
name: Zhang, Lin
name: Leng, Ethan
name: Metzger, Gregory J.
name: Koopmeiners, Joseph S.
Publisher: name: Taylor & Francis
Year: 2021
Subjects: subject: Space Science
subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Biological Sciences not elsewhere classified
subject: Information Systems not elsewhere classified
subject: Mathematical Sciences not elsewhere classified
subject: Cancer

description: Multi-parametric MRI (mpMRI) is a critical tool in prostate cancer (PCa) diagnosis and management. To further advance the use of mpMRI in patient care, computer aided diagnostic methods are under continuous development for supporting/supplanting standard radiological interpretation. While voxel-wise PCa classification models are the gold standard, few if any approaches have incorporated the inherent structure of the mpMRI data, such as spatial heterogeneity and between-voxel correlation, into PCa classification. We propose a machine learning-based method to fill in this gap. Our method uses an ensemble learning approach to capture regional heterogeneity in the data, where classifiers are developed at multiple resolutions and combined using the super learner algorithm, and further account for between-voxel correlation through a Gaussian kernel smoother. It allows any type of classifier to be the base learner and can be extended to further classify PCa sub-categories. We introduce the algorithms for binary PCa classification, as well as for classifying the ordinal clinical significance of PCa for which a weighted likelihood approach is implemented to improve the detection of less prevalent cancer categories. The proposed method has shown important advantages over conventional modeling and machine learning approaches in simulations and application to our motivating patient data.