title: ÐÐ°Ð»Ð¸Ð´Ð°ÑÐ¸Ñ ÑÐµÑÑ-ÑÐ¸ÑÑÐµÐ¼Ñ Ð´Ð»Ñ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐ¸ Ð²Ð¸ÑÑÑÐ° Ð³ÐµÐ¿Ð°ÑÐ¸ÑÐ° Ð¡
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Validation of a test system for the diagnosis of hepatitis C virus
Creators: name: ÐÐµÑÑÐ°ÑÐ¸Ð½Ð°, ÐÐ°ÑÐ³Ð°ÑÐ¸ÑÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: ÐÐ¦Ð Ð² ÑÐµÐ°Ð»ÑÐ½Ð¾Ð¼ Ð²ÑÐµÐ¼ÐµÐ½Ð¸
Ð²Ð¸ÑÑÑ Ð³ÐµÐ¿Ð°ÑÐ¸ÑÐ° Ð¡
Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð°Ñ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐ°
ÑÐµÑÑ-ÑÐ¸ÑÑÐµÐ¼Ð°
Real-time PCR
Hepatitis C virus
molecular diagnostics
test-system

description: ÐÐ° ÑÐµÐ³Ð¾Ð´Ð½ÑÑÐ½Ð¸Ð¹ Ð´ÐµÐ½Ñ ÐÐ¦Ð -Ð°Ð½Ð°Ð»Ð¸Ð· Ð¾Ð´Ð¸Ð½ Ð¸Ð· ÑÐ°Ð¼ÑÑ Ð²Ð¾ÑÑÑÐµÐ±Ð¾Ð²Ð°Ð½Ð½ÑÑ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð² Ð Ð¾ÑÑÐ¸Ð¸. ÐÐ½ Ð´Ð¾Ð²Ð¾Ð»ÑÐ½Ð¾ ÑÐ°ÑÑÐ¾ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·ÑÐµÑÑÑ Ð´Ð»Ñ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐ¸ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¾Ð½Ð½ÑÑ Ð¸ Ð²Ð¸ÑÑÑÐ½ÑÑ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ð¹. Ð­ÑÐ¾Ñ Ð¼ÐµÑÐ¾Ð´ Ð±Ð°Ð·Ð¸ÑÑÐµÑÑÑ Ð½Ð° Ð²ÑÑÐ²Ð»ÐµÐ½Ð¸Ð¸ Ð½ÑÐºÐ»ÐµÐ¸Ð½Ð¾Ð²ÑÑ ÐºÐ¸ÑÐ»Ð¾Ñ Ð² Ð¸Ð½ÑÐµÑÐµÑÑÑÑÐµÐ¼ Ð½Ð°Ñ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð¼ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ðµ. Ð Ð½Ð°ÑÑÐ¾ÑÑÐµÐµ Ð²ÑÐµÐ¼Ñ Ð² Ð¼Ð¸ÑÐµ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ ÑÐ°ÑÑÐµÑ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°ÐµÐ¼Ð¾ÑÑÑ Ð²Ð¸ÑÑÑÐ½ÑÐ¼Ð¸ Ð³ÐµÐ¿Ð°ÑÐ¸ÑÐ°Ð¼Ð¸, Ð½Ð¾ Ð¼Ð½Ð¾Ð³Ð¸Ðµ ÑÐ·Ð½Ð°ÑÑ Ð¾ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ð¸ Ð´Ð¾Ð²Ð¾Ð»ÑÐ½Ð¾ Ð¿Ð¾Ð·Ð´Ð½Ð¾, Ð¿Ð¾ÑÐ¾Ð¼Ñ ÑÑÐ¾ Ð¾Ð½Ð¸ Ð¼Ð¾Ð³ÑÑ Ð¿ÑÐ¾ÑÐµÐºÐ°ÑÑ Ð±ÐµÑÑÐ¸Ð¼Ð¿ÑÐ¾Ð¼Ð½Ð¾. Ð ÑÐ²ÑÐ·Ð¸ Ñ ÑÑÐ¸Ð¼ Ð¿Ð¾ÑÐ²Ð¸Ð»Ð°ÑÑ Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼Ð¾ÑÑÑ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°ÑÑ ÑÐµÑÑ-ÑÐ¸ÑÑÐµÐ¼Ñ Ð´Ð»Ñ ÑÐ°Ð½Ð½ÐµÐ¹ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐ¸ Ð²Ð¸ÑÑÑÐ° ÐÐµÐ¿Ð°ÑÐ¸ÑÐ° Ð¡, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¿ÑÐ¾Ð°Ð½Ð°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°ÑÑ ÐµÑ Ð°Ð½Ð°Ð»Ð¸ÑÐ¸ÑÐµÑÐºÐ¸Ðµ Ð¸ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÑÐµÑÐºÐ¸Ðµ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ¸. Ð¦ÐµÐ»ÑÑ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÐµÑÑÑ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð°Ð½Ð°Ð»Ð¸ÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸Ðº Ð¸ Ð¾ÑÐµÐ½ÐºÐ° ÑÐ°Ð±Ð¾ÑÐ¾ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÐ¸ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð½Ð¾Ð¹ ÑÐµÑÑ-ÑÐ¸ÑÑÐµÐ¼Ñ Ð´Ð»Ñ Ð²ÑÑÐ²Ð»ÐµÐ½Ð¸Ñ Ð ÐÐ Ð²Ð¸ÑÑÑÐ° Ð³ÐµÐ¿Ð°ÑÐ¸ÑÐ° Ð¡. ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: â¢ÐÑÐ¾Ð²ÐµÑÑÐ¸ Ð°Ð½Ð°Ð»Ð¸ÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð¾Ð±Ð·Ð¾Ñ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÑ Ð¿Ð¾ ÑÐµÐ¼Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â¢ÐÐ¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ Ð°Ð½Ð°Ð»Ð¸ÑÐ¸ÑÐµÑÐºÑÑ ÑÑÐ²ÑÑÐ²Ð¸ÑÐµÐ»ÑÐ½Ð¾ÑÑÑ ÑÐµÑÑ-ÑÐ¸ÑÑÐµÐ¼Ñ â¢ÐÑÐ¾Ð²ÐµÑÐ¸ÑÑ Ð²Ð¾ÑÐ¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´Ð¸Ð¼Ð¾ÑÑÑ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð², Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð¿ÑÐ¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ð¸ Ð½Ð°Ð±Ð¾ÑÐ°, Ð¾Ð¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ Ð¿ÑÐµÑÐ¸Ð·Ð¸Ð¾Ð½Ð½Ð¾ÑÑÑ ÑÐ¸ÑÑÐµÐ¼Ñ. â¢ÐÑÐ¾Ð²ÐµÑÐ¸ÑÑ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð¾ÑÐ¸Ð±ÐºÐ¸ Ð¿ÑÐ¸ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð¸Ð¸ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð½Ð° ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÑÐ¸ÑÑÐµÐ¼Ñ, Ð¾Ð¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ ÑÐ¾Ð±Ð°ÑÑÐ½Ð¾ÑÑÑ ÑÐ¸ÑÑÐµÐ¼Ñ. â¢ÐÑÐ¾Ð°Ð½Ð°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°ÑÑ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð·Ð°Ð¼ÐµÐ½Ñ ÑÑÑÑÑ Ð½Ð° ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ¸ ÑÐµÑÑ-ÑÐ¸ÑÑÐµÐ¼Ñ. ÐÐ±ÑÐµÐºÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ â ÑÐ¸Ð½ÑÐµÑÐ¸ÑÐµÑÐºÐ¸Ðµ ÑÑÐ°Ð³Ð¼ÐµÐ½ÑÑ Ð ÐÐ Ð²Ð¸ÑÑÑÐ° Ð³ÐµÐ¿Ð°ÑÐ¸ÑÐ° Ð¡ Ñ ÑÐ¾ÑÐ½ÑÐ¼ ÑÐ¸ÑÐ»Ð¾Ð¼ ÐºÐ¾Ð¿Ð¸Ð¹, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐ»ÑÐ¶Ð°Ñ Ð´Ð»Ñ Ð¿ÑÐ¾Ð²ÐµÑÐºÐ¸ Ð¸ Ð¾ÑÑÐ°Ð±Ð¾ÑÐºÐ¸ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð½Ð¾Ð³Ð¾ Ð½Ð°Ð±Ð¾ÑÐ° Ñ ÑÐµÐ»ÑÑ ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¸Ñ ÑÐ°Ð±Ð¾ÑÐ¾ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÐ¸ ÑÐ¸ÑÑÐµÐ¼Ñ Ð¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸ÐµÐ¼ Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐ¸Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ Ð½Ð° ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²Ð¸Ðµ Ð·Ð°Ð´Ð°Ð½Ð½ÑÐ¼ ÐºÑÐ¸ÑÐµÑÐ¸ÑÐ¼. ÐÑÐ¸ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð¸Ð¸ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±Ð·Ð¾ÑÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ: eLIBRARY.RU, Elsevier ScienceDirect, Scopus, PubMed, NCBI. Ð¡ÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÑÑ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»Ð¸ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ð½Ð¾Ð³Ð¾ Ð¾Ð±ÐµÑÐ¿ÐµÑÐµÐ½Ð¸Ñ Microsoft Excel Ð¸ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ð½Ð¾Ð³Ð¾ Ð¾Ð±ÐµÑÐ¿ÐµÑÐµÐ½Ð¸Ñ Ð°Ð¼Ð¿Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¾ÑÐ° LightCycler 96 (Roche).
description: Today, PCR analysis is one of the most popular research methods in Russia. It is quite often used to diagnose infectious and viral diseases. This method is based on the identification of nucleic acids in the biological material of interest to us. Currently, the incidence of viral hepatitis is actively growing in the world, but many will learn about the disease quite late, because they may be asymptomatic. In this regard, it became necessary to develop a test system for early diagnosis of hepatitis C virus, as well as analyze its analytical and diagnostic characteristics. The purpose of this work is to determine the analytical characteristics and assess the performance of the developed test system for the detection of hepatitis C virus RNA. The tasks that were solved during the study: â¢Conduct an analytical review of the literature on the research topic â¢Determine the analytical sensitivity of the test system â¢Check the reproducibility of the results obtained using the kit, determine the precision of the system. â¢Check the impact of the error during the analysis on the efficiency of the system, determine the robustness of the system. â¢Analyze the impact of raw material replacement on the performance of the test system. The objects of research of this work are synthetic fragments of hepatitis C virus RNA with an exact number of copies, which are used to check and work out the developed set-in order to establish the operability of the system and conduct further studies for compliance with the specified criteria. The following databases were used in the literary review: eLIBRARY.RU, Elsevier ScienceDirect, Scopus, PubMed, NCBI. Statistical processing of experimental data was performed using Microsoft Excel software and amplifier software LightCycler 96 (Roche).