title: ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ ÑÑÐ°Ð½ÑÐ¿Ð¾ÑÑÐ° ÑÑÐ¸ÑÑÐ¾ÑÐ¸ÑÐ¾Ð² Ð² Ð¼Ð¸ÐºÑÐ¾ÑÐ»ÑÐ¸Ð´Ð½ÑÑ ÐºÐ°Ð½Ð°Ð»Ð°Ñ Ð´Ð»Ñ Ð¾ÑÐµÐ½ÐºÐ¸ Ð¸Ñ ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÐ¾ÑÑÐ¾ÑÐ½Ð¸Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Investigation of erythrocyte transport in microfluidic channels to assess their functional state
Creators: name: ÐÑÐµÑÐ°Ð½Ð°Ñ, Ð®Ð»Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Ð¼Ð¸ÐºÑÐ¾ÑÐ»ÑÐ¸Ð´Ð½Ð¾Ðµ ÑÑÑÑÐ¾Ð¹ÑÑÐ²Ð¾
ÑÑÐ¸ÑÑÐ¾ÑÐ¸ÑÑ
Ð¼Ð¸ÐºÑÐ¾ÑÐµÐ¾Ð»Ð¾Ð³Ð¸Ñ
Ð±Ð¸Ð¾ÑÐ¸Ð·Ð¸ÑÐµÑÐºÐ¾Ðµ ÑÐµÐ½Ð¾ÑÐ¸Ð¿Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
Ð¾ÐºÐ¸ÑÐ»Ð¸ÑÐµÐ»ÑÐ½ÑÐ¹ ÑÑÑÐµÑÑ
Ð¸ÑÐµÐ¼Ð¸Ñ-ÑÐµÐ¿ÐµÑÑÑÐ·Ð¸Ñ
microfluidic device
erythrocytes
microrheology
biophysical phenotyping
oxidative stress
ischemia-reperfusion

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description: The given work is devoted to the evaluation of various topologies of microfluidic devices (MFPs) for the analysis of the microrhealogy of rat and human erythrocytes. When developing the MFP, the dimensional characteristics of erythrocytes, the similarity of the topology of the MFP and the microvascular network, and the convenience of recording indicators were considered. Deformability disorders under model conditions were used to evaluate microchips: peroxide-induced oxidative stress (OS) and carotid ischemia-reperfusion (IR). Testing of 4 different MFP topologies was carried out, and then on the selected topology (16-channel microchip), the sensitivity of microchannel sizes was tested (width and length were varied) to violations of microrheology in OS and IR. Changes in hematological and cytological parameters of erythrocytes in OS and IR were confirmed by flow cytometry (volume regulation disorder, lipid bilayer transformation, intracellular esterase activity, accumulation of oxidized forms of hemoglobin). It was found that the rate of passage of erythrocytes through the microchannels of the MFP depends on the ability of cells to deformability in narrow channels - with OS and IR, a subpopulation of erythrocytes with altered transit parameters (slow cells) is formed. The advantages and disadvantages of microcapillaries of various sizes were identified to assess the functional state of rats and humans.