cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Stress-induced development of inflorescence necrosis and bunch-stem necrosis in Vitis vinifera L. in response to environmental und nutritional effects
Creators: name: Keller, M.
name: Koblet, W.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Inflorescences and mature clusters, respectively, of field-grown Muller-Thugau grapevines (Vitis vinifera L.) were immersed in aqueous solutions of the glutamine synthetase (GS) inhibitor phosphinothricin (PPT). Typical symptoms of inflorescence necrosis developed after immersion in 1 or 10 mM PPT, but not in the 0.1 mM treatment. No necrotic symptoms could be induced in mature clusters. In addition, single-node cuttings of field-grown Müller-Thurgau and Pinot noir vines with one cluster, with or without adjacent leaf, were incubated in various solutions with or without PPT at several phenological stages. Necrotic symptoms occurred in all treatments including the H2O control. However, at early stages of development the symptoms appeared earlier than at later stages, and elevated PPT concentrations reduced the development of necrotic manifestations. The addition of NH,NO, to the solution enhanced the appearance of symptoms both in the presence and absence of PPT, while KNO3 did not. In incubation solutions without PPT, metallic cations like Mg2+, Ca2+ and K+ reduced the incidence of bunch-stem necrosis. When cuttings were exposed to different environmental conditions, there was no significant light effect, but wind decreased the incidence of both inflorescence necrosis and bunch-stem necrosis. A leaf, attached to the cluster, or the addition of sucrose to the solution effectively delayed the development of necrotic manifestations. These results indicate that GS is present in rachis, flower and berry tissues and that the buildup of toxic NH4+ levels is involved in the development of both inflorescence necrosis and bunch-stem necrosis. However, NO3- reduction and primary N assimilation appear not to contribute significantly to NH4+ accumulation. This buildup may rather be a secondary effect related to senescence of the tissue due to carbon depletion.
description: VITIS - Journal of Grapevine Research, Vol. 34 No. 3 (1995): Vitis
En eksperimentel undersøgelse af følgeafstande i trafikken under forskellige vejrbetingelser
Creators: name: Troglauer, Thomas
Publisher: name: Proceedings from the Annual Transport Conference at Aalborg University
Year: 2005

description: Følgende er en beskrivelse af et eksperiment udført i 2005 om følgeafstande i trafikken Eksperimentet omhandlede kørsel under forskellige vejrforhold: reduceret sigtbarhed (tåge), reduceret køretøjskontrol (glatføre) og normal kørsel (normal sigtbarhed og normal køretøjskontrol) . Eksperimentet blev udført i en avanceret køresimulator. 12 forsøgspersoner deltog i eksperimentet. Hver forsøgsperson kørte ca. 43 000 meter under hver betingelse. Forsøgspersonerne skulle i løbet af eksperimentet holde en sikker afstand til forankørende bil, der var programmeret til at køre ved 90 km/t under 20 000 meter. I denne artikel analyseres udelukkende kørsel under 90 km/t. De kritiske variable i eksperimentet var tidsafstand, tid til kollision, hastighed samt bremseaktiviteter. Tidsafstanden var lavest ved kørsel under normale vejrbetingelser, mens tidsafstanden var længst under kørsel i glatføre. En-vejs variansanalyse af forsøgsparametrene viste, at der var signifikante forskelle i tidsafstanden mellem alle forskellige vejrbetingelser. Tid til kollision var ikke signifikant forskellig imellem nogle af betingelserne. Hastighed var også signifikant forskellig imellem alle betingelser. Bremsen blev anvendt hyppigst under kørsel i glatføre, men for alle tre betingelser gjaldt, at den blev anvendt i mindre end 2% af alle observationerne. Der blev ikke fundet signifikante i omfanget af bremseaktivitet imellem de forskellige betingelser. Det var forventet, at tidsafstanden ville være længst ved glatføre da forsøgspersonerne qua manglende køretøjskontrol ville kompensere for øget risikooplevelse ved at øge afstanden. Omvendt var det forventet, at tidsafstanden ville være lavest ved tåge, da førere under dette vejrforhold har en tendens til at overvurdere afstanden. Dette blev ikke bekræftet af forsøgsresultaterne.
description: Proceedings from the Annual Transport Conference at Aalborg University, Årg. 12 Nr. 1 (2005): Proceedings from the Annual Transport Conference at Aalborg University
Les parasites des populations de la vigne sylvestre, Vitis vinifera silvestris (Gmelin) Hegi des Pyrénées Atlantiques (France)
Creators: name: Ocete, R.
name: Del Tio, R.
name: Lara, M.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Research Note The pests of the wild grapevine, Vitis vinifera silvestris (Gmelin) Hegi, populations in the Atlantic Pyrenees (France) Several populations of Vitis vinifera silvestris (Gmelin) Hegi have been localized and identified in the Department of Pyrenees Atlantiques (SW of France). Nowadays, this subspecies of V. vinifera is being collected in germplasm banks in order to conserve this genetic material which also could be used in grapevine breeding. According to our field observations, the ''Erineum strain'' of Colomerus vitis (Pagenstecher) (Acari: Eriophyidae) is the pest of the widest distribution and importance in these wild populations.
description: VITIS - Journal of Grapevine Research, Vol. 34 No. 3 (1995): Vitis
Metabolomics reveals the effects of hydroxysafflor yellow A on neurogenesis and axon regeneration after experimental traumatic brain injury
Creators: name: Hu, En
name: Li, Teng
name: Li, Zhilin
name: Su, Hong
name: Yan, Qiuju
name: Wang, Lei
name: Li, Haigang
name: Zhang, Wei
name: Tang, Tao
name: Wang, Yang
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Biochemistry
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Neuroscience
subject: Physiology
subject: Pharmacology
subject: Developmental Biology
subject: Computational Biology

description: Hydroxysafflor yellow A (HSYA) is the main bioactive ingredient of safflower (Carthamus tinctorius L., [Asteraceae]) for traumatic brain injury (TBI) treatment. To explore the therapeutic effects and underlying mechanisms of HSYA on post-TBI neurogenesis and axon regeneration. Male Sprague-Dawley rats were randomly assigned into Sham, controlled cortex impact (CCI), and HSYA groups. Firstly, the modified Neurologic Severity Score (mNSS), foot fault test, hematoxylin-eosin staining, Nissl’s staining, and immunofluorescence of Tau1 and doublecortin (DCX) were used to evaluate the effects of HSYA on TBI at the 14th day. Next, the effectors of HSYA on post-TBI neurogenesis and axon regeneration were screened out by pathology-specialized network pharmacology and untargeted metabolomics. Then, the core effectors were validated by immunofluorescence. HSYA alleviated mNSS, foot fault rate, inflammatory cell infiltration, and Nissl’s body loss. Moreover, HSYA increased not only hippocampal DCX but also cortical Tau1 and DCX following TBI. Metabolomics demonstrated that HSYA significantly regulated hippocampal and cortical metabolites enriched in ‘arginine metabolism’ and ‘phenylalanine, tyrosine and tryptophan metabolism’ including l-phenylalanine, ornithine, l-(+)-citrulline and argininosuccinic acid. Network pharmacology suggested that neurotrophic factor (BDNF) and signal transducer and activator of transcription 3 (STAT3) were the core nodes in the HSYA-TBI-neurogenesis and axon regeneration network. In addition, BDNF and growth-associated protein 43 (GAP43) were significantly elevated following HSYA treatment in the cortex and hippocampus. HSYA may promote TBI recovery by facilitating neurogenesis and axon regeneration through regulating cortical and hippocampal metabolism, BDNF and STAT3/GAP43 axis.
Nachweis und Bestimmung von 2-Aminoacetophenon in vergorenen Modelllösungen
Creators: name: Rapp, A.
name: Versini, G.
name: Engel, L.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Research Note Durch zweidimensionale Gaschromatographie und unter Verwendung eines spezifischen Stickstoffdetektors (NPD) kann 2-Aminoacetophenon noch bis zu Gehalten von 20 ng/1 quantitativ bestimmt werden. In Weinen wurden bisher Gehalte von 20 ng/1 bis 2500 ng/1 nachgewiesen. In Gärversuchen mit synthetischen Nährlösungen wurde eine von der N-Quelle und der Zusammensetzung des Gärsubstrates abhängige Bildung von 2-Aminoacetophenon festgestellt; mit Tryptophan als einziger N-Quelle wurden die größten Mengen 2-Aminoacetophenon gebildet. Determination of 2-aminoacetophenone in fermented model wine solutions Using twodimensional GC in combination with a nitrogen-specific detection, 2-aminoaceto-phenone can be determined quantitatively up to 20 ng/l. In wines concentrations between 20 and 2,500 ng/l could be found. In fermentation experiments with synthetic media a significant influence of the medium composition, especially the nitrogen source, is ascertained. In the case of tryptophane as N source the highest amount of 2-aminoacetophenone is synthesized.
description: VITIS - Journal of Grapevine Research, Vol. 34 No. 3 (1995): Vitis
Antifouling properties of zinc oxide nanorod coatings
Creators: name: Al-Fori, Marwan
name: Dobretsov, Sergey
name: Myo Tay Zar Myint
name: Joydeep Dutta
Publisher: name: Taylor & Francis
Year: 2014
Subjects: Marine Biology

description: In laboratory experiments, the antifouling (AF) properties of zinc oxide (ZnO) nanorod coatings were investigated using the marine bacterium Acinetobacter sp. AZ4C, larvae of the bryozoan Bugula neritina and the microalga Tetraselmis sp. ZnO nanorod coatings were fabricated on microscope glass substrata by a simple hydrothermal technique using two different molar concentrations (5 and 10 mM) of zinc precursors. These coatings were tested for 5 h under artificial sunlight (1060 W m−2 or 530 W m−2) and in the dark (no irradiation). In the presence of light, both the ZnO nanorod coatings significantly reduced the density of Acinetobacter sp. AZ4C and Tetraselmis sp. in comparison to the control (microscope glass substratum without a ZnO coating). High mortality and low settlement of B. neritina larvae was observed on ZnO nanorod coatings subjected to light irradiation. In darkness, neither mortality nor enhanced settlement of larvae was observed. Larvae of B. neritina were not affected by Zn2+ ions. The AF effect of the ZnO nanorod coatings was thus attributed to the reactive oxygen species (ROS) produced by photocatalysis. It was concluded that ZnO nanorod coatings effectively prevented marine micro and macrofouling in static conditions.
Identification of some o-aminophenones as secondary metabolites of Saccharomyces cerevisiae
Creators: name: Ciolfi, G.
name: Garofolo, A.
name: Di Stefano, R.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Research Note During fermentation of a peculiar model medium a strain of Saccharomyces cerevisiae var. cerevisiae (S1C) yeast from our collection was able to produce o-aminoacetophenone as well as other metabolites tentatively identified as o-aminopropiophenone and 3-(o-aminophenyl)-prop-1-en-3-one.
description: VITIS - Journal of Grapevine Research, Vol. 34 No. 3 (1995): Vitis
Keynote address
Creators: name: Tyrninoksa, Seija
Publisher: name: Zenodo
Year: 2020
Subjects: IFRC
Red Cross
Red Crescent
Climate Change

description: An overview of the current challenges and efforts of the International Federation of Red Cross and Red Crescent Societies related to DRR and Preparedness.
description: This e-book is published within the framework of the project IRONORE 2019. The project is co-financed by the European Commission under the contract UCPM-2018-EX-AG-828918.
Qiqilian ameliorates vascular endothelial dysfunction by inhibiting NLRP3-ASC inflammasome activation in vivo and in vitro
Creators: name: Luo, Yuan
name: Tan, Zhenyuan
name: Ye, Yun
name: Ma, Xiaocong
name: Yue, Guihua
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Biochemistry
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Molecular Biology
subject: Physiology
subject: Pharmacology
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Cancer

description: Previous studies have highlighted significant therapeutic effects of Qiqilian (QQL) capsule on hypertension in spontaneously hypertensive rats (SHRs); however, its underlying molecular mechanism remains unclear. We investigated the potential mechanism by which QQL improves hypertension-induced vascular endothelial dysfunction (VED). In vivo, SHRs were divided into four groups (20 per group) and were administered gradient doses of QQL (0, 0.3, 0.6, and 1.2 g/kg) for 8 weeks, while Wistar Kyoto rats were used as normal control. The vascular injury extent, IL-1β and IL-18 levels, NLRP3, ASC and caspase-1 contents were examined. In vitro, the effects of QQL-medicated serum on angiotensin II (AngII)-induced inflammatory and autophagy in human umbilical vein endothelial cells (HUVECs) were assessed. Compared with the SHR group, QQL significantly decreased thickness (125.50 to 105.45 μm) and collagen density (8.61 to 3.20%) of arterial vessels, and reduced serum IL-1β (96.25 to 46.13 pg/mL) and IL-18 (345.01 to 162.63 pg/mL) levels. The NLRP3 and ACS expression in arterial vessels were downregulated (0.21- and 0.16-fold, respectively) in the QQL-HD group compared with the SHR group. In vitro, QQL treatment restored NLRP3 and ASC expression, which was downregulated approximately 2-fold compared with that of AngII-induced HUVECs. Furthermore, QQL decreased LC3II and increased p62 contents (p < 0.05), indicating a reduction in autophagosome accumulation. These effects were inhibited by the autophagy agonist rapamycin and enhanced by the autophagy inhibitor chloroquine. QQL effectively attenuated endothelial injury and inflammation by inhibiting AngII-induced excessive autophagy, which serves as a potential therapeutic strategy for hypertension.
Gastrodin attenuates angiotensin II-induced vascular contraction and MLCK/p-MLC2 pathway activation
Creators: name: Guo, Zhi
name: Yang, Xuan
name: Wu, Meizhu
name: Shen, Aling
name: Li, Jiapeng
name: Zhang, Xiuli
name: Cheng, Ying
name: Xie, Qiurong
name: Peng, Jun
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Biophysics
subject: Medicine
subject: Cell Biology
subject: Molecular Biology
subject: Physiology
subject: FOS: Biological sciences
subject: Pharmacology
subject: Environmental Sciences not elsewhere classified
subject: Science Policy
subject: Biological Sciences not elsewhere classified

description: Gastrodin has been used as antihypertension therapy in China; however, the mechanisms underlying the effects of gastrodin have yet to be fully elucidated. To explore the therapeutic efficiency of gastrodin as an antihypertensive and determine the mechanisms underlying this effect. C57BL/6 mice were continuously administered angiotensin II (Ang II) (500 ng/kg/min) to induce hypertension. Mice were randomly divided into control, Ang II and Ang II + gastrodin groups. Mice received intragastric administration of gastrodin (5 mg/kg) or double distilled water once a day for 4 weeks. Blood pressure, pulse wave velocity (PWV), thickness of the abdominal aorta, pathological morphology and differential expression transcripts (DETs) were assessed. Abdominal aorta rings and primary isolated vascular smooth muscle cells were subjected to Ang II stimulation to induce hypertension as ex vivo and in vitro models, respectively. Vascular ring tension, release of Ca2+ and levels of proteins involved in the myosin light chain kinase (MLCK)/phospho-myosin light chain 2 (p-MLC2) pathway were determined. Gastrodin treatment attenuated increases in blood pressure, PWV and thickness of the abdominal aorta. Treatment with gastrodin resulted in 2785 DETs and the enrichment of vascular contraction and calcium signalling pathways. Gastrodin treatment attenuated Ang II-induced vasoconstriction, produced a norepinephrine-precontracted vasodilation effect (attenuated by verapamil), and reduced intracellular Ca2+ release. Furthermore, gastrodin suppressed activation of the MLCK/p-MLC2 pathway in vivo and in vitro. Gastrodin treatment lowers blood pressure, suppresses Ang II-induced vascular contraction and MLCK/p-MLC2 pathway activation, thereby demonstrating the mechanisms underlying the therapeutic efficacy of gastrodin as an antihypertensive.
Änderungen des Aminosäuregehaltes während der Schaumweinbildung
Creators: name: Malík, F.
name: Šajbidor, J.
name: Buchtová, Viola
name: Minárik, E.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Die Änderung des Aminosäurengehaltes in Sektproben wurde 2, 12 und 24 Monate nach Beginn der Flaschengärung untersucht. Zum Start der sekundäen Gärung wurden zwei ethanoltolerante Saccharomyces cerevisiae - Stämme und drei aktive Trockenhefepräparate verwendet. Nach zwei Monaten konnte ein Konzentrationsrückgang des Großteils der Aminosäuren festgestellt worden. Nur Phenylalanin und Histidin batten zugenommen. Nach 12 Monaten war die Konzentration von Serin, Glycin, Alanin und Valin herabgesetzt, Prolin dagegen angereichert, ebenso wie Leucin und Histidin. Nach zwei Jahren war der Gehalt an Thyrosin, Histidin und Lysin, Leucin und Isoleucin erhöht, während eine Tendenz zur Verringerung der Konzentration von Prolin, Valin und Arginin registriert wurde. Die essentielle Aminosäure Methionin war nach 24 Monaten in allen Sektproben verbraucht. Changes of the amounts of amino acids during sparkling wine production Changes of the amino acid content after 2, 12 and 24 months in sparkling wine samples have been investigated. Two liquid and three active dry wine yeast preparations of Saccharomyces cerevisiae strains were used for the secondary fermentation. After two months a decrease of most amino acids was observed, only the histidine content was increased. The concentration of serine, glycine, alanine and valine decreased after 12 months. In this phase, on the other hand, the proline, leucine and histidine contents increased. After two years the content of thyrosine, histidine and lysine as well as leucine and isoleucine in sparkling wines raised, while proline, valine and asparagine showed a decrease compared with the initial concentrations. The essential amino acid methionine was utilized in all sparkling wine samples after 24 months.
description: VITIS - Journal of Grapevine Research, Vol. 34 No. 3 (1995): Vitis
Transcriptomic analysis reveals the regulatory mechanism underlying the indirubin-mediated amelioration of dextran sulfate sodium-induced colitis in mice
Creators: name: Liu, Zhe
name: Zhang, Jin-ru
name: Huang, Yong-xiang
name: Li, Xue-ying
name: Zhu, Hai-peng
name: Yang, Rui-yi
name: Chen, Song
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Biochemistry
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Molecular Biology
subject: Pharmacology
subject: Biotechnology
subject: Environmental Sciences not elsewhere classified
subject: Chemical Sciences not elsewhere classified
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Infectious Diseases
subject: FOS: Health sciences

description: Aryl hydrocarbon receptor (AhR) agonists are potential therapeutic agents for ulcerative colitis (UC). Indirubin (IDR), which is a natural AhR ligand approved for leukemia treatment, ameliorates dextran sulfate sodium (DSS)-induced colitis in mice. However, the therapeutic mechanisms of IDR are unknown, limiting its application. This study explores the therapeutic mechanisms of IDR in DSS-induced colitis using transcriptomic analysis. Male BALB/c mice were categorized to six groups: normal, DSS model (2% DSS), IDR treatment (10, 20 and 40 mg/kg), and sulfasalazine (520 mg/kg) groups. The drugs were intragastrically administered for 7 consecutive days. The disease activity index (DAI) was recorded. After euthanasia, the colon length was measured, and histopathological examination, immunohistochemistry staining using F4/80, and colonic transcriptomic analysis were conducted. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and western blotting (WB) were conducted to verify our findings. Compared with DSS, IDR treatment decreased the DAI score by 64.9% and increased colon length by 26.2%. Moreover, it alleviated mucosal injury and reduced macrophage infiltration. Transcriptomic analysis identified several downregulated genes (Igkvs and Nlrp3), as well as Nlrp3/Il1β and hemoglobin gene networks, after IDR treatment. The abundances of NF-κB p65, NLRP3, IL-1β, and HBA decreased by 69.1, 59.4, 81.1, and 83.0% respectively, after IDR treatment. Apart from the well-documented NF-κB signalling pathway, IL-17A, and NLRP3-IL-1β, the suppression of haemoglobin-induced lipid peroxidation could be a previously unknown mechanism of IDR. Our study can help improve its application for UC treatment.
Effect of grapevine cultivar, strain of Xylophilus ampelinus and culture medium on in vitro development of bacterial necrosis
Creators: name: Peros, J. P.
name: Berger, G.
name: Ride, M.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Research Note Factorial experiments were designed to study the response of in vitro cultivated grapevine after inoculation with Xylophilus ampelinus, the causal agent of bacterial necrosis. Significant effects of culture medium, cultivar and strain were demonstrated. The possible. uses for the in vitro test are discussed.
description: VITIS - Journal of Grapevine Research, Vol. 34 No. 3 (1995): Vitis
title: Groseillier à Maquereau :
title: Principes directeurs pour la conduite de l’examen de la distinction, de l’homogénéité et de la stabilité.
titleType: Subtitle
Creators: name: (:Unas)
Publisher: name: Unknown
Year: 2011

description: Ces principes directeurs (“principes directeurs d’examen”) visent à approfondir les principes énoncés dans l’introduction générale (document TG/1/3) et dans les documents TGP qui s’y rapportent afin de donner des indications concrètes détaillées pour l’harmonisation de l’examen de la distinction, de l’homogénéité et de la stabilité (DHS) et, en particulier, à identifier des caractères convenant à l’examen DHS et à la production de descriptions variétales harmonisées.
title: Historiographie du Néolithique
Publisher: name: Université Toulouse - Jean Jaurès
Year: 2014

description: Ce séminaire expose la mutation de la recherche préhistorique au cours des quatre dernières décennies, et plus précisément l’histoire de la recherche néolithique française qui est celle d’une discipline longtemps minorée devenue l’un des secteurs les plus dynamiques de l’archéologie hexagonale.
Panax notoginseng saponins (PNS) attenuate Th17 cell differentiation in CIA mice via inhibition of nuclear PKM2-mediated STAT3 phosphorylation
Creators: name: Shen, Mei-Yu
name: Di, Yu-Xi
name: Wang, Xiang
name: Tian, Feng-Xiang
name: Zhang, Ming-Fei
name: Qian, Fei-Ya
name: Jiang, Bao-Ping
name: Zhou, Xue-Ping
name: Zhou, Ling-Ling
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Biochemistry
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Physiology
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: Immunology
subject: FOS: Clinical medicine
subject: Developmental Biology
subject: Cancer
subject: Hematology

description: Rheumatoid arthritis (RA) is an autoimmune disease with aberrant Th17 cell differentiation. Panax notoginseng (Burk.) F. H. Chen (Araliaceae) saponins (PNS) have an anti-inflammatory effect and can suppress Th17 cell differentiation. To investigate mechanisms of PNS on Th17 cell differentiation in RA, and the role of pyruvate kinase M2 (PKM2). Naive CD4+T cells were treated with IL-6, IL-23 and TGF-β to induce Th17 cell differentiation. Apart from the Control group, other cells were treated with PNS (5, 10, 20 μg/mL). After the treatment, Th17 cell differentiation, PKM2 expression, and STAT3 phosphorylation were measured via flow cytometry, western blots, or immunofluorescence. PKM2-specific allosteric activator (Tepp-46, 50, 100, 150 μM) and inhibitor (SAICAR, 2, 4, 8 μM) were used to verify the mechanisms. A CIA mouse model was established and divided into control, model, and PNS (100 mg/kg) groups to assess an anti-arthritis effect, Th17 cell differentiation, and PKM2/STAT3 expression. PKM2 expression, dimerization, and nuclear accumulation were upregulated upon Th17 cell differentiation. PNS inhibited the Th17 cells, RORγt expression, IL-17A levels, PKM2 dimerization, and nuclear accumulation and Y705-STAT3 phosphorylation in Th17 cells. Using Tepp-46 (100 μM) and SAICAR (4 μM), we demonstrated that PNS (10 μg/mL) inhibited STAT3 phosphorylation and Th17 cell differentiation by suppressing nuclear PKM2 accumulation. In CIA mice, PNS attenuated CIA symptoms, reduced the number of splenic Th17 cells and nuclear PKM2/STAT3 signaling. PNS inhibited Th17 cell differentiation through the inhibition of nuclear PKM2-mediated STAT3 phosphorylation. PNS may be useful for treating RA.
2019 AARP Arizona Caregiving Survey
Creators: name: American Association Of Retired Persons (AARP)
Publisher: name: Roper Center for Public Opinion Research
Year: 2022

description: Current/former unpaid caregiver (8); care activities (20); caregiving effects on employment (6); financial/home life impacts of caregiving(16); caregiving effects on mental health/stress (9); importance of caregivers/providing care to ensure older residents/loved ones can live independently in their own home (2); likelihood of providing care again in the future (3); support/oppose state income tax credit for family caregivers (2).
Development of metabolic engineering approaches to regulate the content of total phenolics, antiradical activity and organic acids in callus cultures of the highbush blueberry (Vaccinium corymbosum L.)
Creators: name: Ramata-Stunda, A.
name: Valkovska, V.
name: Borodušķis, M.
name: Livkiša, D.
name: Kaktiņa, E.
name: Silamiķele, B.
name: Borodušķe, A.
name: Pentjušs, A.
name: Rostoks, N.
Publisher: name: Agronomy Research
Year: 2020
Subjects: blueberry
Vaccinium corymbosum
total phenolic content
antiradical activity
organic acids
callus cultures
article

description: Blueberry (Vaccinium corymbosum L.) is increasingly cultivated to produce high quality berries for consumption and potential applications in medicine, nutrition and as industrial precursors. Seasonal availability sets limitations on chemical compound isolation from cultivated plants. Biotechnological solutions, such as tissue cultures and metabolic engineering, can provide sufficient amounts of plant material with reasonably high metabolite levels, which may be adjusted by different strategies. Here, we describe our approach to modifying total phenolic content (TPC), antiradical activity (ARA) and amounts of selected organic acids in in vitro cultures of two varieties of V. corymbosum by varying the growth media. TPC, ARA and acid levels were determined in mature leaves of field-grown plants and in stable callus cultures derived from leaves of varieties ‘Bluecrop’ and ‘Duke’ grown on Murashige-Skoog (MS) and Woody plant (WP) media supplemented with varying concentrations and combinations of different plant growth hormones. TPC varied from 83 mg g -1 dry weight (DW) to 142 mg g -1 DW in leaves of ‘Bluecrop’ and ‘Duke’, respectively, and correlated with their ARA with ‘Duke’ at the lead. For callus cultures the highest ARA, as well as the highest TPC of 94 mg g -1 DW was observed in ‘Bluecrop’ grown on WP medium with 2,4-dichlorophenoxyacetic acid (2,4-D). High level of quinic acid was found in the mature leaves of all tested varieties, while callus cultures exhibited relative increase in amounts of malic, succinic and citric acids instead. Oxalic acid was found only in callus cultures.
title: 01_2034
Creators: name: Wesley Baldwin, John
name: Baldwin, John W.
Publisher: name: NAKALA - https://nakala.fr (Huma-Num - CNRS)
publisherIdentifier: https://ror.org/04ces3204
publisherIdentifierScheme: ROR
Year: 2018
Subjects: subject: LaMOP
subject: Laboratoire de Médiévistique Occidentale de Paris

description: L'ensemble de ces notes ont été crée par John Baldwin lors d'une de ses premières périodes de résidence à Paris. Ces documents comprennent tous les magistri qu’il avait pu rencontrer dans ses sources. Aux XIIème et XIIIème siècles, les universités n'existant pas encore, le titre de magister s'il évoque certainement une compétence n'est pas rattaché de façon systématique au passage par les écoles. De surcroît, les magistris sont le plus souvent désignés par leur seul prénom. La lecture de ces données est donc difficile et suppose un long travail pour tenter d'identifier un individu auquel on pourrait rattacher plusieurs mentions. John Baldwin avait donc renoncé à utiliser ces données pour donner un prolongement à sa magnifique étude sur les gradués d'université et leur emploi par les Capétiens et les Plantagenêts. Néanmoins, il a conservé ces données et elles sont potentiellement très utile dans le cadre de Studium, comme le sont toutes les mentions dans les volumes des Fasti Ecclesiae Gallicanae de chanoines portant ce titre de magister sans autres précisions. L'ampleur des dépouillements rend ces données très utiles et nous devons une grande reconnaissance à John Baldwin pour avoir pris la peine de faire photocopier ses fiches et de nous les avoir transmises amicalement.
Les données ainsi que les images des fiches sont accessibles sur le site Internet à l'adresse suivante : http://studium-baldwin.lamop.fr/.
Les transcriptions des fiches ont été effectuées par des étudiants de Paris 1 Panthéon - Sorbonne mais elles n'ont pas été corrigées par la suite.
description: Relation: https://lamop.hypotheses.org/
Effects of fungal root infection on the vigor of grapevines infested by root-feeding grape phylloxera
Creators: name: Omer, A. D.
name: Granett, J.
name: De Benedictis, J. A.
name: Walker, M. A.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: The role of fungal pathogens in damage of grapevines (Vitis vinifera L.; cv. Chardonnay) associated with grape phylloxera (Daktulosphaira vitifoliae FITCH) was investigated. Seven different genera of secondary fungi were isolated from surface-disinfected feeding sites of phylloxera but none from surface-disinfected root tissues undamaged by phylloxera. Damage in vines infested with phylloxera and infected with Fusarium solani (MART.) or with F. solani and Pythium ultimum TROW. was significantly greater than damage in vines infested with phylloxera only. In a greenhouse experiment, total biomass was reduced by 16% in vines infested with phylloxera and 24 to 29% in vines infested with phylloxera and infected with fungus in comparison with control vines. Chlorophyll content, average internode length, shoot biomass, and root biomass in the uninfested, uninfected vines were significantly greater than vines infested with phylloxera or vines infested with phylloxera and infected with F. solani or P. ultimum, or both. Preventative treatment with metalaxyl, benomyl or copper quinolinolate fungicides significantly decreased damage in phylloxera-infested vines in comparison with untreated vines. The implications of this research with respect to management of grape phylloxera are discussed.
description: VITIS - Journal of Grapevine Research, Vol. 34 No. 3 (1995): Vitis
Nomenclature of grapevine leafroll-associated putative closteroviruses
Creators: name: Boscia, D.
name: Greif, C.
name: Gugerli, P.
name: Martelli, G. P.
name: Walter, B.
name: Gonsalves, D.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Comparative immunoenzymatic (ELISA), immunoelectron microscopic (IEM) and immunoblotting tests were carried out with antisera produced in different laboratories and commercial diagnostic kits on closterolike viruses reported in the literature under the name of grapevine corky bark-associated virus (GCBaV) and grapevine leafroll-associated viruses IIa and IIb (GLRaV IIa and GLRaV IIb). The results of these studies have established that GCBaV is the same as GLRaV IIb and that both viruses are apparently identical to an isolate of GLRaV-2 identified in France, whose designation as the authentic GLRaV 2 is proposed. GLRaV IIa is serologically distinct from all known clostero-like viruses of the grapevine and, therefore, the provisional name of grapevine leafroll-associated Virus 6 (GLRaV-6) is suggested for it.
description: VITIS - Journal of Grapevine Research, Vol. 34 No. 3 (1995): Vitis
Comparaison de différentes techniques de greffage vis-à-vis de leur efficacité de transmission virale sur vigne
Creators: name: Lahogue, Florence
name: Boulard, G.
name: Schneider, C.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Dans le but d'effectuer un criblage rapide d'un grand nombre de variétes de vigne vis-à-vis de leur résistance aux virus, nous avons recherché quelle méthode d'inoculation était la plus adaptée . L'inoculation mécanique d'un virus à la vigne étant difficile à obtenir, nous avons comparé différentes techniques de greffage: greffage sur bois, greffage en vert avec porte-greffe préalablement enraciné ou non, chaque fois, deux positions possibles pour l'inoculum: greffon ou porte-greffe. Les résultats de cette étude indiquent que le greffage sur bois avec l'inoculum en position de porte-greffe correspond à la méthode la plus efficace. Toutefois, le greffage en vert avec porte-greffe non enraciné donne également de bons résultats, toujours avec l'inoculum en position de porte-greffe. Etant donnés les avantages que présente la greffe en vert en ce qui concerne la surface necessaire à sa réalisation et sa vitesse de réalisation, ainsi que son utilisation possible tout au long de l'année, elle paraît tout à fait adaptée pour inoculer un grand nombre de variétés. Comparison of the viral transmission efficiency of different grafting techniques on grapevines With the objective of screening a great number of grapevine varieties for their resistance to virus diseases, we looked for the most appropriate technique of inoculation. As the mechanical inoculation of a virus to the grapevine succeeds only under special conditions, we compared different grafting techniques: dormant grafting and green grafting, the last with and without previously rooted rootstock. For each of these three methods, the inoculum was used as the stock on one hand, as the scion on the other hand. This study proved that the dormant grafting technique using the inoculum as the rootstock is the most efficient technique. Nevertheless, good results were obtained by using the green grafting technique without previously rooting the rootstock, the inoculum being also used as the rootstock. As the green grafting method doesn't require a great area, is not time-consuming and can be performed throughout the year, it seems therefore to be a very convenient method of inoculating a great number of varieties.
description: VITIS - Journal of Grapevine Research, Vol. 34 No. 3 (1995): Vitis
Response surface for biodiesel production from soybean oil by ethylic route
Creators: name: Souza, L.A.
name: Silva, F.C.
name: Maria, A.C.L.
name: Belem, A.L.
name: Cecchin, D.
name:  Barros, M.M.
Publisher: name: Agronomy Research
Year: 2020
Subjects: biofuels
ethanol
optimization
transesterification
article

description: Petroleum has been the most consumed energy source in the world, but it tends to run out due its non-renewable character. Among biofuels, biodiesel has emerged as the main candidate to substitute petroleum diesel. The present study aimed to identify the maximum yield point of biodiesel production by generating a response surface using molar ratio, temperature and agitation time as independent variables, and yield as a dependent variable. From the response surface, it is observed that the increase in temperature and reaction time leads to reduced yield. The configuration that resulted in maximum yield of 93.30% was 12:1 molar ratio, 30 °C temperature and 30-minute reaction time. From the chromatographic analysis it was possible to identify five different fatty acids in the composition of the biodiesels. Total saturated fatty acids (palmitic and stearic acids) ranged from 41.53% to 42.09% and total unsaturated fatty acids including monounsaturated and polyunsaturated fatty acids (oleic, linoleic and linolenic acids) ranged from 57.92% to 58.48%. According to the results of the physicochemical analyses, the specific mass at 68°F is in agreement with Brazilian, American and European specifications, ranging from 877.46 kg m-3 to 879.64 kg m-3 . The kinematic viscosity at 104 °F ranged from 4.49 mm² s -1 to 4.82 mm² s -1 . The acid value obtained did not vary within the limits established by the norms, and values between 0.54 and 2.74 mg KOH g -1 were observed.
Mechanistic exploration of Yiqi Liangxue Shengji prescription on restenosis after balloon injury by integrating metabolomics with network pharmacology
Creators: name: Mao, Tianshi
name: Xie, Long
name: Guo, Yanqiong
name: Ji, Xiang
name: Wan, Jie
name: Cui, Xiaoyun
name: Fan, Qian
name: Liu, Wei
name: Wang, Shuai
name: Han, Wenbo
name: Lin, Qian
name: Jia, Wenhao
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Biochemistry
subject: Space Science
subject: Medicine
subject: Cell Biology
subject: Physiology
subject: FOS: Biological sciences
subject: Pharmacology
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Marine Biology

description: Yiqi Liangxue Shengji prescription (YQLXSJ) is a traditional Chinese medicine (TCM) formula that has long been used for treatment after percutaneous coronary intervention (PCI). To investigate the putative pharmacological mechanism of YQLXSJ on restenosis through an integrated approach utilizing metabolomics and network pharmacology. Forty male Sprague–Dawley rats were divided into sham, model, YQLXSJ, and positive groups. YQLXSJ group received the treatment of YQLXSJ (6 g/kg/d, i.g.) and the positive group was treated with atorvastatin (2 mg/kg/d, i.g.). After 4 weeks, the improvement in intimal hyperplasia was evaluated by ultrasound, H&E staining, and immunofluorescence. UPLC–MS/MS technology was utilized to screen the differential metabolites. Network pharmacology was conducted using TCMSP, GeneCards, and Metascape, etc., in combination with metabolomics. Eventually, the core targets were acquired and validated. Compared to models, YQLXSJ exhibited decreased intima-media thickness on ultrasound (0.23 ± 0.02 mm vs. 0.20 ± 0.01 mm, p < 0.01) and reduced intima thickness by H&E (30.12 ± 6.05 μm vs. 14.32 ± 1.37 μm, p < 0.01). We identified 18 differential metabolites and 5 core targets such as inducible nitric oxide synthase (NOS2), endothelial nitric oxide synthase (NOS3), vascular endothelial growth factor-A (VEGFA), ornithine decarboxylase-1 (ODC1) and group IIA secretory phospholipase A2 (PLA2G2A). These targets were further confirmed by molecular docking and ELISA. This study confirms the effects of YQLXSJ on restenosis and reveals some biomarkers. TCM has great potential in the prevention and treatment of restenosis by improving metabolic disorders.
Gemcitabine enhances pharmacokinetic exposure of the major components of Danggui Buxue Decoction in rat via the promotion of intestinal permeability and down-regulation of CYP3A for combination treatment of non-small cell lung cancer
Creators: name: Xu, Xin
name: Sun, Xi-yang
name: Chang, Ming
name: Hu, Zhao-liang
name: Cheng, Ting-ting
name: Hang, Tai-jun
name: Song, Min
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Biochemistry
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Physiology
subject: Pharmacology
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Developmental Biology
subject: Cancer
subject: Mental Health

description: Danggui Buxue Decoction (DBD), a traditional Chinese medicine formula, has the potential to enhance the antitumor effect of gemcitabine in non-small cell lung cancer (NSCLC) treatment by increasing gemcitabine’s active metabolites. However, whether gemcitabine affects the pharmacokinetics of DBD’s major components remains unclear. This study evaluates the herb–drug interaction between DBD’s major components and gemcitabine and validates the underlying pharmacokinetic mechanism. The pharmacokinetics of 3.6 g/kg DBD with and without a single-dose administration of 50 mg/kg gemcitabine was investigated in Sprague-Dawley rats. The effects of gemcitabine on intestinal permeability, hepatic microsomal enzymes in rat tissues, and CYP3A overexpressing HepG2 cells were determined using western blot analysis. The combination of gemcitabine significantly altered the pharmacokinetic profiles of DBD’s major components in rats. The Cmax and AUC of calycosin-7-O-β-d-glucoside notably increased through sodium-glucose transporter 1 (SGLT-1) expression promotion. The AUC of ligustilide and ferulic acid was also significantly elevated with the elimination half-life (t1/2) prolonged by 2.4-fold and 7.8-fold, respectively, by down-regulating hepatic CYP3A, tight junction proteins zonula occludens-1 (ZO-1) and occludin expression. Gemcitabine could modulate the pharmacokinetics of DBD’s major components by increasing intestinal permeability, enhancing transporter expression, and down-regulating CYP3A. These findings provide critical information for clinical research on DBD as an adjuvant for NSCLC with gemcitabine and help make potential dosage adjustments more scientifically and rationally.
Promising anti-Helicobacter pylori and anti-inflammatory metabolites from unused parts of Phoenix dactylifera CV ‘Zaghloul’: in vitro and in silico study
Creators: name: Elhefni, Nada
name: Ebada, Sherif S.
name: Abdel-Aziz, Marwa M.
name: Marwan, El-Sayed M.
name: El-Sharkawy, Saleh
name: El-Neketi, Mona
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Biochemistry
subject: Space Science
subject: Microbiology
subject: FOS: Biological sciences
subject: Pharmacology
subject: Environmental Sciences not elsewhere classified
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Infectious Diseases
subject: FOS: Health sciences

description: Date palm waste is an agricultural waste that accumulates in massive amounts causing serious pollution and environmental problems. Date palm trees, Phoenix dactylifera Linn CV ‘Zaghloul’ (Arecaceae) grown in Egypt, leave behind waste products that were investigated to produce compounds with anti-Helicobacter pylori and anti-inflammatory activities. Chromatographic workup of P. dactylifera aqueous methanol extract derived from fibrous mesh and fruit bunch (without fruit) afforded a new sesquiterpene lactone derivative, phodactolide A (1), along with ten known compounds (2–11), primarily identified as polyphenols. Chemical structures were unambiguously elucidated based on mass and 1D/2D NMR spectroscopy. All isolated compounds were assessed for their activities against H. pylori using broth micro-well dilution method and clarithromycin as a positive control. The anti-inflammatory response of isolated compounds was evaluated by inhibiting cyclooxygenase-2 enzyme using TMPD Assay followed by an in silico study to validate their mechanism of action using celecoxib as a standard drug. Compounds 4, 6 and 8–10 exhibited potent anti-H. pylori activity with MIC values ranging from 0.48 to 1.95 µg/mL that were comparable to or more potent than clarithromycin. For COX-2 inhibitory assay, 4, 7 and 8 revealed promising activities with IC50 values of 1.04, 0.65 and 0.45 μg/mL, respectively. These results were verified by molecular docking studies, where 4, 7 and 8 showed the best interactions with key amino acid residues of COX-2 active site. The present study characterizes a new sesquiterpene lactone and recommends 4 and 8 for future in vivo studies as plausible anti-ulcer remedies.
Rowan powder based acidifying additive acidifying additive - an alternative to sourdough in the rye-wheat bread production
Creators: name: Dubrovskaya, N.
name: Parakhina, O.
name: Lokachuk, M.
name: Savkina, O.
name: Kuznetsova, L.
Publisher: name: Agronomy Research
Year: 2020
Subjects: bread making
plant acidifying additive
sodium diacetate
accelerated bakery technologies
article

description: Rye is an important part of the cereal food culture in the Russia and Nordic, Baltic and Eastern European countries. Rye bread is often made of whole-grain flour using long-time sourdough. In Russia, rye bread began to be produced less and less often due to the complexity and duration of its technology. Therefore, the development of fast, natural and safe technologies is relevant. The aim of the research was to develop a nutritional acidifying additive based on plant materials (rowan powder, botanical species Sorbus aucuparia) for accelerated rye-wheat bread technology. With an increase in the new plant additive dosage above 3.5%, the dough lifting capacity deteriorated. The bread specific volume and the crumb compressibility deteriorated when additive dosage was higher than 3.5%. It all may be due to the acidity suppression of yeast activity in the dough. Taste and smell was also better in bread with new additive due to the rowan powder chemical composition. The optimum dosage of new additive rowan powder in rye-wheat bread formulation was 3.5% by weight of the flour. Usage of new additive with 0.1% of sodium diacetate allowed slowing down moulding. New acidifying with rowan powder allowed to create accelerated rye-wheat bread technology and to get bread with high consumer properties.
Mechanisms of dihydromyricetin against hepatocellular carcinoma elucidated by network pharmacology combined with experimental validation
Creators: name: Zhang, Shuo
name: Shi, Ya-Ning
name: Gu, Jia
name: He, Peng
name: Ai, Qi-Di
name: Zhou, Xu-Dong
name: Wang, Wei
name: Qin, Li
Publisher: name: Taylor & Francis
Year: 2023
Subjects: Biochemistry
Pharmacology
Chemical Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Cancer

description: Dihydromyricetin (DMY) is extracted from vine tea, a traditional Chinese herbal medicine with anti-cancer, liver protection, and cholesterol-lowering effects. This study investigated the mechanism of DMY against hepatocellular carcinoma (HCC). Potential DMY, HCC, and cholesterol targets were collected from relevant databases. PPI networks were created by STRING. Then, the hub genes of co-targets, screened using CytoHubba. GO and KEGG pathway enrichment, were performed by Metascape. Based on the above results, a series of in vitro experiments were conducted by using 40–160 μM DMY for 24 h, including transwell migration/invasion assay, western blotting, and Bodipy stain assay. Network pharmacology identified 98 common targets and 10 hub genes of DMY, HCC, and cholesterol, and revealed that the anti-HCC effect of DMY may be related to the positive regulation of lipid rafts. Further experiments confirmed that DMY inhibits the proliferation, migration, and invasion of HCC cells and reduces their cholesterol levels in vitro. The IC50 is 894.4, 814.4, 467.8, 1,878.8, 151.8, and 156.9 μM for 97H, Hep3B, Sk-Hep1, SMMC-7721, HepG2, and Huh7 cells, respectively. In addition, DMY downregulates the expression of lipid raft markers (CAV1, FLOT1), as well as EGFR, PI3K, Akt, STAT3, and Erk. The present study reveals that DMY suppresses EGFR and its downstream pathways by reducing cholesterol to disrupt lipid rafts, thereby inhibiting HCC, which provides a promising candidate drug with low toxicity for the treatment of HCC.