title: Ð¤ÑÐ½ÐºÑÐ¸Ð¾Ð½Ð°Ð»ÑÐ½Ð°Ñ Ñ Ð°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ° ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ð°Ð»ÑÐ½ÑÑ Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÑ ÑÑÑÐ¾Ð¼Ð°Ð»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð¿ÑÐ¸ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ Ð² ÑÑÐµÑÐ¾Ð¸Ð´Ð°Ñ Ð²Ð½ÑÑÑÐ¸ Ð¿Ð¾ÑÐ¸ÑÑÐ¾Ð¹ ÑÐµÐ»Ð»ÑÐ»Ð¾Ð·Ð½Ð¾Ð¹ Ð¼Ð°ÑÑÐ¸ÑÑ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Functional characteristic of human endometrial mesenchymal stromal cells cultivated in spheroids within the porous cellulose matrix
Creators: name: ÐÑÐ½ÐµÐµÐ², ÐÐ²Ð°Ð½
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Ð¤Ð¸Ð·Ð¸ÐºÐ°
ÐÐ»ÐµÑÐºÐ° (Ð±Ð¸Ð¾Ð».)
3D ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ð¹
3D cell culture
endometrium

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description: The development of three-dimensional cell culture systems is extremely important for the development of modern biomedical technologies. Cultivation in 3D configuration makes it possible to accurately reproduce the microenvironment cells are situated in vivo, which determines to a greater extent their morphofunctional characteristics. The use of 3D cultures is an effective approach to design relevant in vitro models of human tissues and organs. Within this work, a three-dimensional culture of human mesenchymal stromal cells of endometrial origin (eMSC) in spheroids inside a porous cellulose matrix was created and characterized. Using fluorescent research methods, it has been shown that spheroids from human eMSCs obtained noninvasively from menstrual fluid can be cultured within a decellularized plant-derived scaffold for a long time, which makes the developed technique very accessible. Endometrial MSCs in the created 3D system retain viability, phenotype and proliferative activity. Their functional characteristics are also preserved. We suggest that the developed culture system can be successfully used for tissue engineering, in particular, to create a functional model of endometrial tissue. In this regard, this culture has great potential for use in regenerative biomedicine.