Trichoderma beijingense K. Chen & W. Y. Zhuang HMAS 2017, sp. nov.
Creators: name: Chen, Kai
name: Zhuang, Wen-Ying
Publisher: name: Zenodo
Year: 2017
Subjects: Biodiversity
Taxonomy
Fungi
Ascomycota
Pezizomycetes
Pezizales
Pezizaceae
Trichoderma
Trichoderma beijingense

description: Trichoderma beijingense K. Chen & W.Y. Zhuang, sp. nov. Fig 3   
Fungal Names: FN570276.  
 Etymology: The specific epithet refers to the type locality.   
 Diagnosis. Colony fast-growing on CMD, PDA and SNA at 25 °C. Conidiophores trichoderma-like, phialides narrowly lageniform, conidia green, subglobose, less commonly globose.   
On CMD after 72 h colony radius 50–51 mm and mycelium covering the plate after 4 d at 25 °C. Colony hyaline, radial, margin well defined, aerial hyphae common, absent at the colony center. Conidiation noted after 3 d, first formed on aerial hyphae, conidial pustules noted after 5 d, spreading abundant around the periphery of the colony, hemispherical, loose, 1–2 mm diam, white, turning green after 8 d. Chlamydospores common. No distinct odor, no diffusing pigment observed.  
On PDA after 72 h colony radius 48–50 mm and mycelium covering the plate after 4 d at 25 °C. Colony hyaline, zonate, aerial hyphae abundant. Conidiation noted after 3 d, effuse in aerial hyphae or in densely disposed shrubs, shrubs appearing in 3–4 concentric zones. Conidiophores symmetrical, trichoderma-like. Phialides typically solitary or paired, sometimes in whorls of 3, narrowly lageniform, rarely subulate, 8.9–12.4(–15.2) × 2.1–3.1 μm, l/ w 3.2 –5.7, 1.4–2.2 μm wide at the base (n = 40). Conidia green, subglobose, less commonly globose, 3.3–4.2 × 2.8–3.3 μm, l/w (1.0–)1.1–1.5 (n=40). Chlamydospores common. No distinct odor, no diffusing pigment observed.  
On SNA after 72 h colony radius 20–21 mm and mycelium covering the plate after 9 d at 25 °C. Colony hyaline, margin slightly lobed and ill-defined, aerial hyphae inconspicuous. Conidiation noted after 3 d, formed in pustules, pustules spreading around the original inoculum, pulvinate, flat, adhered firmly to surface, up to 8 mm in diam, green with white margin. Chlamydospores common. No distinct odor, no diffusing pigment observed.  
 Strains examined: CHINA. Beijing: Yanqing, Yudushan, elev. 926 m, isolated from soil, 8 July 2015, K. Chen TC727 (holotype HMAS 248804, ex-type culture CGMCC 3.18322). ibid., K. Chen TC728 (HMAS 248805).   
 Notes: Trichoderma beijingense is characterized by its flat, pulvinate pustules on SNA, which is uncommon in Trichoderma. The species appears to be a separate terminal branch in the phylogenetic tree, and was grouped with T. atroviride, T. paratroviride and T. subviride, which is poorly supported (Fig 1, MPBP/MLBP/BIPP = 67%/74%/–). The above fungi show morphological distinctions, and cannot be confused. In comparison, T. atroviride has shorter phialides [(4–)6–10(–12) μm], and grows faster on SNA at 25 °C; T. paratroviride differs in having globose conidia; and T. subviride can be easily distinguished by its much shorter phialides (Jaklitsch 2011; Jaklitsch & Voglmayr 2015; Qin & Zhuang 2016b).
description: Published as part of Chen, Kai & Zhuang, Wen-Ying, 2017, Seven soil-inhabiting new species of the genus Trichoderma in the Viride clade, pp. 28-46 in Phytotaxa 312 (1) on pages 33-36, DOI: 10.11646/phytotaxa.312.1.2, http://zenodo.org/record/13701723