title: Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð¼ÐµÑÐ¾Ð´Ð¸ÐºÐ¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ ÐÐ¦Ð Ñ Ð¾Ð±ÑÐ°ÑÐ½Ð¾Ð¹ ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸ÐµÐ¹ Ð² Ð¾Ð´Ð½Ð¾Ð¹ Ð¿ÑÐ¾Ð±Ð¸ÑÐºÐµ Ð´Ð»Ñ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð¼Ð¾Ð»ÐµÐºÑÐ» Ð¼Ð¸ÐºÑÐ¾Ð ÐÐ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Development of a technique for conducting PCR with reverse transcription in one test tube for the quantitative analysis of microRNA molecules
Creators: name: ÐÐ¸Ð½ÑÐ¾Ð½ÐµÐ· Ð¡Ð¾ÑÐ¾, ÐÑÐ°Ð¸Ð°Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð¼Ð¸ÐºÑÐ¾Ð ÐÐ
ÑÐ¸ÑÐ¾Ð²Ð¸Ð´Ð½Ð°Ñ Ð¶ÐµÐ»ÐµÐ·Ð°
Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÑÐµÑÐºÐ°Ñ Ð·Ð½Ð°ÑÐ¸Ð¼Ð¾ÑÑÑ
microRNA
thyroid gland
diagnostic significance

description: Ð¦ÐµÐ»ÑÑ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÐ»Ð¾ÑÑ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð¼ÐµÑÐ¾Ð´Ð° ÐÐ¦Ð  Ñ Ð¾Ð±ÑÐ°ÑÐ½Ð¾Ð¹ ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸ÐµÐ¹ Ð² Ð¾Ð´Ð½Ð¾Ð¹ Ð¿ÑÐ¾Ð±Ð¸ÑÐºÐµ Ð´Ð»Ñ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð¼Ð¾Ð»ÐµÐºÑÐ» Ð¼Ð¸ÐºÑÐ¾Ð ÐÐ Ð¿ÑÐ¸ Ð²Ð¸Ð·ÑÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ ÑÐ¾Ð»Ð»Ð¸ÐºÑÐ»ÑÑÐ½Ð¾Ð¹ Ð°Ð´ÐµÐ½Ð¾Ð¼Ñ Ð¸ ÑÐ¾Ð»Ð»Ð¸ÐºÑÐ»ÑÑÐ½Ð¾Ð¹ ÐºÐ°ÑÑÐ¸Ð½Ð¾Ð¼Ñ ÑÐ¸ÑÐ¾Ð²Ð¸Ð´Ð½Ð¾Ð¹ Ð¶ÐµÐ»ÐµÐ·Ñ. ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: 1. ÐÐ·ÑÑÐµÐ½Ð¸Ðµ Ð¸ÑÑÐ¾ÑÐ½Ð¸ÐºÐ¾Ð² Ð¾ ÑÐ¾Ð»Ð¸ Ð¼Ð¾Ð»ÐµÐºÑÐ» Ð¼Ð¸ÐºÑÐ¾Ð ÐÐ Ð² Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐµ Ð¾Ð¿ÑÑÐ¾Ð»ÐµÐ²ÑÑ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ð¹. 2. ÐÑÐ±Ð¾Ñ Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼Ð¾Ð³Ð¾ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð° ÐºÐ»Ð¸Ð½Ð¸ÑÐµÑÐºÐ¸Ñ Ð¾Ð±ÑÐ°Ð·ÑÐ¾Ð² ÑÐ¸ÑÐ¾Ð²Ð¸Ð´Ð½Ð¾Ð¹ Ð¶ÐµÐ»ÐµÐ·Ñ (ÑÐ¾Ð»Ð»Ð¸ÐºÑÐ»ÑÑÐ½Ð¾Ð¹ Ð°Ð´ÐµÐ½Ð¾Ð¼Ñ Ð¸ ÑÐ¾Ð»Ð»Ð¸ÐºÑÐ»ÑÑÐ½Ð¾Ð¹ ÐºÐ°ÑÑÐ¸Ð½Ð¾Ð¼Ñ). 3. Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð¸ Ð¾Ð¿ÑÐ¸Ð¼Ð¸Ð·Ð°ÑÐ¸Ñ Ð¿ÑÐ¾ÑÐ¾ÐºÐ¾Ð»Ð° Ð´Ð»Ñ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ ÐÐ¦Ð  Ñ Ð¾Ð±ÑÐ°ÑÐ½Ð¾Ð¹ ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸ÐµÐ¹ Ð² Ð¾Ð´Ð½Ð¾Ð¹ Ð¿ÑÐ¾Ð±Ð¸ÑÐºÐµ. 4. Ð¡ÑÐ°Ð²Ð½ÐµÐ½Ð¸Ðµ Ð¿Ð¾ÐºÐ°Ð·Ð°ÑÐµÐ»ÐµÐ¹ ÑÑÐ²ÑÑÐ²Ð¸ÑÐµÐ»ÑÐ½Ð¾ÑÑÐ¸ Ð¸ ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐ½Ð¾ÑÑÐ¸ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð²ÑÐ±ÑÐ°Ð½Ð½ÑÑ Ð¼Ð¾Ð»ÐµÐºÑÐ» Ð² ÐºÐ»Ð¸Ð½Ð¸ÑÐµÑÐºÐ¸Ñ Ð¾Ð±ÑÐ°Ð·ÑÐ°Ñ Ð¼ÐµÑÐ¾Ð´Ð°Ð¼Ð¸ ÐÐ¢-ÐÐ¦Ð . Ð Ð°Ð±Ð¾ÑÐ° Ð±ÑÐ»Ð° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð² Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ ÑÑÐ±ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¹ Ñ Ð³ÑÑÐ¿Ð¿Ð¾Ð¹ Ð¾Ð½ÐºÐ¾ÑÐ½Ð´Ð¾ÐºÑÐ¸Ð½Ð¾Ð»Ð¾Ð³Ð¸Ð¸ ÐÐÐÐ¦ Ð¾Ð½ÐºÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¸Ð¼. Ð. Ð. ÐÐµÑÑÐ¾Ð²Ð°. ÐÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½Ð°Ñ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° ÑÐ¸ÑÑÐµÐ¼Ñ ÐÐ¢-ÐÐ¦Ð  Ñ Ð¾Ð±ÑÐ°ÑÐ½Ð¾Ð¹ ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸ÐµÐ¹ Ð² Ð¾Ð´Ð½Ð¾Ð¹ Ð¿ÑÐ¾Ð±Ð¸ÑÐºÐµ ÑÐ¸Ð½ÑÐµÑÐ¸ÑÐµÑÐºÐ¸Ð¼Ð¸ Ð°Ð½Ð°Ð»Ð¾Ð³Ð°Ð¼Ð¸ Ð¼Ð¸ÐºÑÐ¾Ð ÐÐ miR-146a Ð¸ miR-185. ÐÐ°ÑÐµÑÐ¸Ð°Ð»Ñ Ð¸ Ð¼ÐµÑÐ¾Ð´Ñ: 12 Ð¾Ð±ÑÐ°Ð·ÑÐ¾Ð² Ð¼Ð¸ÐºÑÐ¾Ð ÐÐ, Ð²ÑÐ´ÐµÐ½Ð½Ð¾Ð¹ Ð¸Ð· FFPE ÑÐ¾Ð»Ð¸ÐºÑÐ»ÑÑÐ½Ð¾Ð¹ Ð°Ð´ÐµÐ½Ð¾Ð¼Ñ Ð¸ 12 FFPE ÑÐ¾Ð»Ð¸ÐºÑÐ»ÑÑÐ½Ð¾Ð¹ ÐºÐ°ÑÑÐ¸Ð½Ð¾Ð¼Ñ Ð´Ð»Ñ Ð¾ÑÐµÐ½ÐºÐ¸ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð·Ð½Ð°ÑÐ¸Ð¼Ð¾ÑÑÐ¸. ÐÐ½Ð°Ð»Ð¸Ð· ÑÑÐ¾Ð²Ð½Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ 24 Ð¾Ð±ÑÐ°ÑÐ·Ð¾Ð² Ð¼Ð¸ÐºÑÐ¾Ð ÐÐ Ð±ÑÐ» Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ ÐÐ¢-ÐÐ¦Ð  ÑÐ¾ ÑÑÐ°Ð½Ð´Ð°ÑÑÐ½ÑÐ¼ Ð¿ÑÐ¾ÑÐ¾ÐºÐ¾Ð»Ð¾Ð¼ Ð¸ Ð¿ÑÐ¾ÑÐ¾ÐºÐ¾Ð»Ð¾Ð¼ Ð´Ð»Ñ ÐÐ¢-ÐÐ¦Ð  Ð² Ð¾Ð´Ð½Ð¾Ð¹ Ð¿ÑÐ¾Ð±Ð¸ÑÐºÐµ. Ð ÐµÐ·ÑÐ»ÑÑÐ°ÑÑ: Ð­ÐºÑÐ¿ÑÐµÑÑÐ¸Ñ miR-146a Ð¸ miR-185 Ð² ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð½Ð¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ñ ÐÐ¢-ÐÐ¦Ð  Ð² Ð¾Ð´Ð½Ð¾Ð¹ Ð¿ÑÐ¾Ð±Ð¸ÑÐºÐµ Ð¾ÐºÐ°Ð·Ð°Ð»Ð°ÑÑ Ð¿ÑÐ°ÐºÑÐ¸ÑÐµÑÐºÐ¸ Ð¾Ð´Ð¸Ð½Ð°ÐºÐ¾Ð²ÑÐ¼ ÑÐ¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸ÐµÐ¼ Ð¿ÑÐ¾ÑÐµÐ´ÑÑÑ ÑÑÐ°Ð½Ð´Ð°ÑÑÐ½Ð¾Ð³Ð¾ Ð¿ÑÐ¾ÑÐ¾ÐºÐ¾Ð»Ð° ÐÐ¢-ÐÐ¦Ð . ÐÑÐ²Ð¾Ð´Ñ: ÐÐ°Ð»ÑÐ½ÐµÐ¹ÑÐ¸Ðµ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¼ÐµÑÐ¾Ð´Ð° ÐÐ¢-ÐÐ¦Ð  Ð² Ð¾Ð´Ð½Ð¾Ð¹ Ð¿ÑÐ¾Ð±Ð¸ÑÐºÐµ Ñ ÑÐµÐ»ÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¼Ð¸ÐºÑÐ¾Ð ÐÐ Ð² Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸ÑÑ Ð©Ð Ð´Ð»Ñ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ¸ Ð½Ð¾Ð²ÑÑ Ð¿Ð¾Ð´ÑÐ¾Ð´Ð¾Ð² Ðº Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐµ ÑÑÐ¸Ñ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ñ. ÐÑÐ¸ Ð½Ð°Ð¿Ð¸ÑÐ°Ð½Ð¸Ð¸ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±Ð·Ð¾ÑÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ°ÐºÐ¸Ðµ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ, ÐºÐ°Ðº NIH, Spandidos publications, Journal of molecular endocrinology.
description: The aim of this work was to develop a single-tube reverse transcription PCR method for quantitative analysis of microRNA molecules in imaging follicular adenoma and follicular thyroid carcinoma. Tasks that were solved during the research: 1. Study of sources on the role of microRNA molecules in the diagnosis of tumor diseases. 2. Selection of the required number of clinical samples of the thyroid gland (follicular adenoma and follicular carcinoma). 3. Development and optimization of a protocol for conducting PCR with reverse transcription in a single tube. 4. Comparison of sensitivity and specificity of the analysis of selected molecules in clinical samples by RT-PCR methods The work was performed in the laboratory of subcellular Technologies with the Oncoendocrinology group of the N.N. Petrov National Research Institute of Oncology. An experimental development of a RT-PCR system with reverse transcription in one tube with synthetic miR-146a and miR-185 microRNA analogues has been carried out. Materials and methods: 12 samples of microRNA extracted from FFPE follicular adenoma and 12 FFPE follicular carcinoma for diagnostic significance. The expressionâs level of 24 microRNA samples was analyzed by RT-PCR with a standard protocol and a protocol for one step RT-PCR in one test tube. Results: The expression of miR-146a and miR-185 in the developed RT-PCR system in one test tube turned out to be almost identical in comparison of the procedure of the standard RT-PCR protocol. Conclusions: Further use of the single-tube RT-PCR method for the study of microRNAs in the thyroid glandâs cancer to develop new approaches to the diagnosis of these diseases. Databases such as NIH, Spandidos publications, and Journal of Molecular endocrinology were used when writing the literary review.