Cellular plasticity in response to suppression of storage proteins in the Brassica napus embryo
Creators: name: Rolletschek, Hardy
name: Schwender, Jörg
name: König, Christina
name: Chapman, Kent D.
name: Romsdahl, Trevor
name: Lorenz, Christin
name: Braun, Hans-Peter
name: Denolf, Peter
name: Van Audenhove, Katrien
name: Munz, Eberhard
name: Heinzel, Nicolas
name: Ortleb, Stefan
name: Rutten, Twan
name: McCorkle, Sean
name: Borysyuk, Taras
name: Guendel, André
name: Shi, Hai
name: Auwermeulen, Michiel Vander
name: Bourot, Stephane
name: Borisjuk, Ljudmilla
Publisher: name: Rockville, Md. : American Society of Plant Physiologists
Year: 2020
Subjects: subject: amino acid
subject: carbon
subject: cruciferin protein, Brassica napus
subject: membrane lipid
subject: napin
subject: napin protein, Brassica napus
subject: nitrogen
subject: oleosin protein, Brassica napus
subject: plant antigen
subject: plant protein
subject: seed storage protein
subject: cytology
subject: gene expression regulation
subject: genetics
subject: metabolism
subject: nuclear magnetic resonance spectroscopy
subject: plant cell
subject: plant seed
subject: rapeseed
subject: RNA interference
subject: transgenic plant
subject: 2S Albumins, Plant
subject: Amino Acids
subject: Antigens, Plant
subject: Brassica napus
subject: Carbon
subject: Gene Expression Regulation, Plant
subject: Magnetic Resonance Spectroscopy
subject: Membrane Lipids
subject: Nitrogen
subject: Plant Cells
subject: Plant Proteins
subject: Plants, Genetically Modified
subject: RNA Interference
subject: Seed Storage Proteins
subject: Seeds
subject: Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
subject: Dewey Decimal Classification::500 | Naturwissenschaften::580 | Pflanzen (Botanik)
subject: Dewey Decimal Classification::500 | Naturwissenschaften::540 | Chemie

description: The tradeoff between protein and oil storage in oilseed crops has been tested here in oilseed rape (Brassica napus) by analyzing the effect of suppressing key genes encoding protein storage products (napin and cruciferin). The phenotypic outcomes were assessed using NMR and mass spectrometry imaging, microscopy, transcriptomics, proteomics, metabolomics, lipidomics, immunological assays, and flux balance analysis. Surprisingly, the profile of storage products was only moderately changed in RNA interference transgenics. However, embryonic cells had undergone remarkable architectural rearrangements. The suppression of storage proteins led to the elaboration of membrane stacks enriched with oleosin (sixfold higher protein abundance) and novel endoplasmic reticulum morphology. Protein rebalancing and amino acid metabolism were focal points of the metabolic adjustments to maintain embryonic carbon/nitrogen homeostasis. Flux balance analysis indicated a rather minor additional demand for cofactors (ATP and NADPH). Thus, cellular plasticity in seeds protects against perturbations to its storage capabilities and, hence, contributes materially to homeostasis. This study provides mechanistic insights into the intriguing link between lipid and protein storage, which have implications for biotechnological strategies directed at improving oilseed crops.