Additional file 1 of A common mechanism of detoxification for lambda-cyhalothrin and abamectin in Cydia pomonella
Creators: name: Ju, Di
name: Hu, Chao
name: Li, Peirong
name: Gao, Ping
name: Li, Yuting
name: Yang, Xueqing
Publisher: name: figshare
Year: 2024
Subjects: subject: Genetics
subject: FOS: Biological sciences

description: Additional file 1: Figure S1. GluCl mRNA (A) and amino acid sequence (B) of C. pomonella, the specific primer positions were underlined in red, the PCR product was in red letter, positions of V263I, A309V, I321T, and G326E mutations were highlight with yellow, red, blue, and green backgrounds. Figure S2. Alignment of transmembrane domain 2 and 3 (TM2 and TM3) of C. pomonella and other species, including Plutella xylostella (PxGluCl-V263 and PxGluCl-263I, GenBank: ACT09139.1,), Bombyx mori (BmGluCl, AGW27406.1), Drosophila melanogaster (DmGluCl, AAG40735.1), Musca domestica (MdGluCl, BAD16657.1) and Tetranychus urticae (TuGluCl, NP_001310061.1). Figure S3. Nucleotide sequence of GluCl and VGSC of C. pomonella in SS and ZW_R. Figure S4. The most enriched Gene ontology (GO) of co-downregulated expressed genes in LCT-treated or AM-treated group. A In each section of the Venn diagram is the number of co-downregulated genes for LD10 and LD30 of LCT treatment at different time intervals, compared to the control. Gene GO enriched DEGs of C. pomonella after exposure to two low different doses of LCT at all different time intervals. The Y-axis represents sub GO terms of biological process (BP), cellular component (CC), and molecular function (MF). The significance of pathways is shown in q-value (color bar), rich factor (X-axis), and circle size, showing the number of genes regulated. B In each section of the Venn diagram is the number of co-downregulated genes for LD10 and LD30 of AM treatment at different time intervals, compared to the control. GO enriched DEGs of C. pomonella after exposure to two different doses of AM at all different time intervals. The Y-axis represents sub Go terms of biological process (BP), cellular component (CC), and molecular function (MF). The significance of pathways is shown in q-value (color bar), rich factor (X-axis), and circle size, showing the number of genes regulated. LCT lambda-cyhalothri; AM abamectin. Figure S5. Phylogenetic tree of P450 genes in Cydia pomonella. Amino acid sequences from Bombyx mori (Bm), Spodoptera litura (Sl), Spodoptera exigua (Se), Spodoptera frugiperda (Sf) Manduca sexta (Ms), Plutella xylostella (Px), Zygaena filipendulae (Zf) and Helicoverpa armigera (Ha) were aligned using ClustalW ( http://www.mbio.ncsu.edu/bioedit/bioedit.html ). Phylogenetic analysis was constructed using the maximum likelihood with IQ-TREE. Four P450 clans are clustered together and are indicated by different colors. Figure S6. Protein location of the 72 P450s in C. pomonella. Genes names and chromosome numbers are showed on the right and left of the bar, respectively. Figure S7. Phylogenetic tree of CarE genes in Cydia pomonella. Amino acid sequences from Bombyx mori (Bm) and Chilo suppressalis (Csup) were aligned using ClustalW ( http://www.mbio.ncsu.edu/bioedit/bioedit.html ). Phylogenetic analysis was constructed using the maximum likelihood with IQ-TREE. Three CarE clans are clustered together and are indicated by different colors. Figure S8. Protein location of the 75 CarEs in C. pomonella. Genes names and chromosome numbers are showed on the right and left of the bar, respectively. Table S1 Primer sequence used for PCR or RT-qPCR. Table S2 Summary statistics of the transcriptome of C. pomonella. Table S3 Top 10 up-regulated unigenes. Table S4 Comparison of the number of gene in P450 subfamilies from various insect species. Table S5 Comparison of the number of gene in CarE subfamilies from various insect species.