cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Impact of the COVID-19 pandemic on the work of social workers
Creators: name: Schell-Kiehl, Ines
name: Laurens, Melissa
name: Ketelaar, Nicole
name: Sommerfeld, Peter
name: Hess, Nadja
name: Bühler, Sarah
name: Meyer, Nikolaus
name: Franz, Sebastian
Publisher: name: Journal of Comparative Social Work
Year: 2022
Subjects: subject: social work profession, international comparison, COVID-19 pandemic, use of technology, contact professional and client, working conditions for professionals
subject: 300 - Sozialwissenschaften

description: The COVID-19 pandemic has had a major impact on everyone’s life. Like many other professionals, social workers have been forced to adapt to these new working conditions and new challenges in order to support clients during the pandemic, as new needs have arisen. Together with professional associations from three nations (Germany, Switzerland and the Netherlands), we used a coordinated approach to explore the consequences of the pandemic for social work professionals. This study was conducted during the most severe contact and hygiene restrictions of the second wave of the COVID-19 pandemic in the winter of 2020/2021. The data addresses the changes perceived by social work professionals in relation to their contact and communication with clients, the use of digital technology in the context of work, the professional response in terms of innovation, the working conditions and the psychosocial risks they face. MethodsCross-sectional data was collected from 7,241 social workers in Germany, Switzerland and the Netherlands through online surveys. ResultsThe results show an increase in the workload of professional social workers and compounding problems of clients, together with a negative impact of the COVID-19 pandemic on communication and contact with clients. All of this takes place within the framework of changing working conditions and contexts. Our data shows that the use of digital technologies does not cause bigger problems for most of the participating social workers. It should in fact be noted that professionals have many positive associations with the use of digital technology in general. ConclusionsThere are both remarkable and alarming results concerning the mental health of social workers and their working conditions, as well as the position of the social work profession in general.
Cosmological constant, supersymmetry, nonassociativity, and Big Numbers
Creators: name: Dzhunushaliev, Vladimir
Publisher: name: arXiv
Year: 2015
Subjects: subject: General Relativity and Quantum Cosmology (gr-qc)
subject: Mathematical Physics (math-ph)
subject: FOS: Physical sciences

description: The nonassociative generalization of supersymmetry is considered. It is shown that the associator of four supersymmetry generators has the coefficient $\sim \hbar/ \ell_0^2$ where $\ell_0$ is some characteristic length. Two cases are considered: (a) $\ell_0^{-2}$ coincides with the cosmological constant; (b) $\ell_0$ is the classical radius of electron. It is also shown that the scaled constant is of the order of $10^{-120}$ for the first case and $10^{-30}$ for the second case. The possible manifestation and smallness of nonassociativity is discussed.
description: essential changes in the text
title: Using light meters to investigate the light-myopia association
title: a literature review of devices and research methods
titleType: AlternativeTitle
Creators: name: Hönekopp, Astrid
name: Weigelt, Sarah
Publisher: name: TU Dortmund
Year: 2023
Subjects: subject: Light exposure
subject: Outdoor exposure
subject: Myopia
subject: Wearables
subject: Lux
subject: 360
subject: 370

description: With the increasing prevalence of myopia, evaluating its relationship with objective light exposure as a potential adjustable environmental factor in myopia development has been an emerging research field in recent years. From a thorough literature search, we identify ten wearable light meters from human studies on light exposure and myopia and present an overview of their parameters, thereby demonstrating the wide between-device variability and discussing its implications. We further identify 20 publications, including two reanalyses, reporting investigations of light-myopia associations with data from human subjects wearing light meters. We thoroughly review the publications with respect to general characteristics, aspects of data collection, participant population, as well as data analysis and interpretation, and also assess potential patterns regarding the absence or presence of light-myopia associations in their results. In doing so, we highlight areas in which more research is needed as well as several aspects that warrant consideration in the study of light exposure and myopia.
description: Clinical ophthalmology;Vol. 17, 2023, pp 2737—2760
Switching the magnetization in quantum antiferromagnets
Creators: name: Uhrig, Götz S.
name: Bolsmann, Katrin
name: Khudoyberdiev, Asliddin
Publisher: name: TU Dortmund
Year: 2023
Subjects: subject: Magnetization switching
subject: 530

description: The orientation of the order parameter of quantum magnets can be used to store information in a dense and efficient way. Switching this order parameter corresponds to writing data. To understand how this can be done, we study a precessional reorientation of the sublattice magnetization in an (an)isotropic quantum antiferromagnet induced by an applied magnetic field. For this intriguing nonequilibrium issue, we introduce a description including the leading quantum and thermal fluctuations, namely time-dependent Schwinger boson mean-field theory, because this theory allows us to describe both ordered phases and the phases in between them, as is crucial for switching. An activation energy has to be overcome, requiring a minimum applied field ht that is given essentially by the spin gap. It can be reduced significantly for temperatures approaching the Néel temperature, facilitating switching. The time required for switching diverges when the field approaches ht, which is the signature of an inertia in the magnetization dynamics. The temporal evolution of the magnetization and of the energy reveals signs of dephasing. The switched state has lost a part of its coherence because the magnetic modes do not evolve in phase.
description: PRX quantum;Band 4. 2023, Heft 3, Artikel-ID 030332
Modellierung historischer Reisezeiten im motorisierten Individualverkehr in Deutschland
Creators: name: Killer, Veronika
name: Guth, Dennis
name: Holz-Rau, Christian
name: Axhausen, Kay W.
Publisher: name: TU Dortmund
Year: 2010
Subjects: subject: 710

description: Die Straßeninfrastruktur ist während der letzten 40 Jahre stark ausgebaut worden. Der vorliegende Bericht beschreibt die Datengewinnung zur Abbildung der historischen Reisezeiten und Netzdistanzen im motorisierten Individualverkehr von 1970 bis 2006 in Deutschland.
description: The road infrastructure has been greatly expanded during the past 40 years. This report describes the data collection and modelling of the historical travel times and network distances of private transport from 1970 to 2006 in Germany.
description: Arbeitspapiere des Fachgebiets Verkehrswesen und Verkehrsplanung;19
Fine-Grained Sports, Yoga, and Dance Postures Recognition: A Benchmark Analysis
Creators: name: Bera, Asish
name: Nasipuri, Mita
name: Krejcar, Ondrej
name: Bhattacharjee, Debotosh
Publisher: name: arXiv
Year: 2023
Subjects: subject: Computer Vision and Pattern Recognition (cs.CV)
subject: FOS: Computer and information sciences

description: Human body-pose estimation is a complex problem in computer vision. Recent research interests have been widened specifically on the Sports, Yoga, and Dance (SYD) postures for maintaining health conditions. The SYD pose categories are regarded as a fine-grained image classification task due to the complex movement of body parts. Deep Convolutional Neural Networks (CNNs) have attained significantly improved performance in solving various human body-pose estimation problems. Though decent progress has been achieved in yoga postures recognition using deep learning techniques, fine-grained sports, and dance recognition necessitates ample research attention. However, no benchmark public image dataset with sufficient inter-class and intra-class variations is available yet to address sports and dance postures classification. To solve this limitation, we have proposed two image datasets, one for 102 sport categories and another for 12 dance styles. Two public datasets, Yoga-82 which contains 82 classes and Yoga-107 represents 107 classes are collected for yoga postures. These four SYD datasets are experimented with the proposed deep model, SYD-Net, which integrates a patch-based attention (PbA) mechanism on top of standard backbone CNNs. The PbA module leverages the self-attention mechanism that learns contextual information from a set of uniform and multi-scale patches and emphasizes discriminative features to understand the semantic correlation among patches. Moreover, random erasing data augmentation is applied to improve performance. The proposed SYD-Net has achieved state-of-the-art accuracy on Yoga-82 using five base CNNs. SYD-Net's accuracy on other datasets is remarkable, implying its efficiency. Our Sports-102 and Dance-12 datasets are publicly available at https://sites.google.com/view/syd-net/home.
description: 12 pages, 12 figures, 10 tables
Global CNS gene delivery and evasion of anti-AAV-neutralizing antibodies by intrathecal AAV administration in non-human primates
Creators: name: Gray, S J
name: Nagabhushan Kalburgi, S
name: Jude Samulski, R
name: McCown, T J
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2013

description: Injection of AAV into the cerebrospinal fluid (CSF) offers a means to achieve widespread transgene delivery to the central nervous system, where the doses can be readily translated from small to large animals. In contrast to studies with other serotypes (AAV2, AAV4, AAV5) in rodents, we report that a naturally-occurring capsid (AAV9) and rationally-engineered capsid (AAV2.5) are able to achieve broad transduction throughout the brain and spinal cord parenchyma following a single injection into the CSF (via cisterna magna or lumbar cistern) in non-human primates (NHP). Using either vector at a dose of ~2×1012 vg per 3-6 kg animal, approximately 2% of the entire brain and spinal cord was transduced, covering all regions of the CNS. AAV9 in particular displayed efficient transduction of spinal cord motor neurons. The peripheral organ biodistribution was highly reduced compared to intravascular delivery, and the presence of circulating anti-AAV neutralizing antibodies up to a 1:128 titer had no inhibitory effect on CNS gene transfer. Intra-CSF delivery effectively translates from rodents to NHPs, which provides encouragement for the use of this approach in humans to treat motor neuron and lysosomal storage diseases.
q-deformed noncommutative cat states and their nonclassical properties
Creators: name: Dey, Sanjib
Publisher: name: arXiv
Year: 2015
Subjects: subject: Quantum Physics (quant-ph)
subject: High Energy Physics - Theory (hep-th)
subject: Mathematical Physics (math-ph)
subject: FOS: Physical sciences

description: We study several classical like properties of q-deformed nonlinear coherent states as well as nonclassical behaviours of q-deformed version of the Schrodinger cat states in noncommutative space. Coherent states in q-deformed space are found to be minimum uncertainty states together with the squeezed photon distributions unlike the ordinary systems, where the photon distributions are always Poissonian. Several advantages of utilising cat states in noncommutative space over the standard quantum mechanical spaces have been reported here. For instance, the q-deformed parameter has been utilised to improve the squeezing of the quadrature beyond the ordinary case. Most importantly, the parameter provides an extra degree of freedom by which we achieve both quadrature squeezed and number squeezed cat states at the same time in a single system, which is impossible to achieve from ordinary cat states.
description: 12 pages, 5 figures
Pitting Corrosion Resistance of the 316L Stainless Steel Welds: Influenced of Oxygen Content in the Protective Gas
Creators: Maroufkhani, Mohammmad
Khodabandeh, Alireza
Radu, Iulian
Jahazi, Mohammad
Publisher: name: University of Alberta Library
Year: 2022

Conference Abstract. Part of the Proceedings of the Canadian Society for Mechanical Engineering International Congress 2022.
Performance benchmarking of an ultra-low vibration laboratory to host a commercial millikelvin scanning tunnelling microscope
Creators: name: Que, Yande
name: Kumar, Amit
name: Lodge, Michael S.
name: Tong, Zhengjue
name: Fai, Marcus Lai Kar
name: Tao, Wei
name: Cui, Zhenhao
name: Shivajirao, Ranjith
name: Jia, Junxiang
name: Lee, Siew Eang
name: Weber, Bent
Publisher: name: arXiv
Year: 2023
Subjects: subject: Superconductivity (cond-mat.supr-con)
subject: Other Condensed Matter (cond-mat.other)
subject: Strongly Correlated Electrons (cond-mat.str-el)
subject: FOS: Physical sciences

description: Ultra-low temperature scanning tunnelling microscopy and spectroscopy (STM/STS) achieved by dilution refrigeration can provide unrivalled insight into the local electronic structure of quantum materials and atomic-scale quantum systems. Effective isolation from mechanical vibration and acoustic noise is critical in order to achieve ultimate spatial and energy resolution. Here, we report on the design and performance of an ultra-low vibration (ULV) laboratory hosting a customized but otherwise commercially available 40mK STM. The design of the vibration isolation consists of a T-shaped concrete mass block (55t), suspended by actively controlled pneumatic springs, and placed on a foundation separated from the surrounding building in a "room-within-a-room" design. Vibration levels achieved are meeting the VC-M vibration standard at >3 Hz, reached only in a limited number of laboratories worldwide. Measurement of the STM's junction noise confirms effective vibration isolation on par with custom built STMs in ULV laboratories. In this tailored low-vibration environment, the STM achieves an energy resolution of 43ueV (144 mK), promising for the investigation and control of quantum matter at atomic length scales.
Three-dimensional multipotent progenitor cell aggregates for expansion, osteogenic differentiation and ‘in vivo’ tracing with AAV vector serotype 6
Creators: name: Zhang, L
name: Hirsch, M L
name: Park, Y
name: Ferreira, J R
name: Ko, C-C
name: Samulski, R J
name: Hu, W-S
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2013

description: Multipotent adult progenitor cells (MAPC) are bone marrow-derived stem cells with a high growth rate suitable for therapeutical applications as three-dimensional (3D) aggregates. Combined applications of osteogenically differentiated MAPC (OD-MAPC) aggregates and adeno-associated viral vectors (AAV) in bone bioengineering are still deferred until information regarding expansion technologies, osteogenic potential, and AAV cytotoxicity and transduction efficiency is better understood. In this study, we tested whether self-complementary AAV (scAAV) can potentially be used as a gene delivery system in a OD-MAPC-based “in vivo” bone formation model in the craniofacial region. Both expansion of rat MAPC (rMAPC) and osteogenic differentiation with dexamethasone were also tested in 3D aggregate culture systems “in vitro” and “vivo”. Rat MAPCs (rMAPCs) grew as undifferentiated aggregates for 4 days with a population doubling time of 37h. After expansion, constant levels of Oct4 transcripts, and Oct4 and CD31 surface markers were observed, which constitute a hallmark of rMAPCs undifferentiated stage. Dexamethasone effectively mediated rMAPC osteogenic differentiation by inducing the formation of a mineralized collagen type I network, and facilitated the activation of the wnt/β-catenin, a crucial pathway in skeletal development. To investigate the genetic modification of rMAPCs grown as 3D aggregates prior to implantation, scAAV serotypes 2, 3, and 6 were evaluated. scAAV6 packaged with the enhanced green fluorescent protein expression cassette efficiently mediated long-term transduction (10 days) “in vitro” and “vivo”. The reporter transduction event allowed the tracing of OD-rMAPC (induced by dexamethasone) aggregates following OD-rMAPC transfer into a macro-porous hydroxyapatite scaffold implanted in a rat calvaria model. Furthermore, the scAAV6-transduced OD-rMAPC generated a bone-like matrix with a collagenous matrix rich in bone specific proteins (osteocalcin and osteopontin) in the scaffold macro-pores 10 days post-implantation. Newly formed bone was also observed in the interface between native bone and scaffold. The collective work supports future bone tissue engineering applications of 3D MAPC cultures for expansion, bone formation, and the ability to genetically alter these cells using scAAV vectors.
Le cadre juridique de la transformation de la  SARL en SA et de la SA en SARL au Maroc
Creators: name: SAIDI Yasmina
name: FAOUZI ZIZI Sofia
Publisher: name: Zenodo
Year: 2023

description: La transformation de la forme juridique de l'entreprise est l'opération par laquelle une société d'une forme sociale donnée adopte une nouvelle forme sociale. C'est une stratégie de gestion du changement destinée à faire évoluer  l'entreprise conformément aux objectifs visés pour l'avenir. 
Les besoins de l'entreprise peuvent ne plus être aujourd'hui ce qu'ils étaient hier, le cadre de son organisation, valable hier, peut se révéler aujourd'hui trop étroit, inadapté, incompatible avec les impératifs de son développement et parfois, même de sa survie.
Les avantages et les inconvénients inhérents à la structure et au fonctionnement du type de société initialement choisi, l'apparition de nouvelles formes de sociétés, le désir d'échapper à une fiscalité de plus en plus écrasante, la nécessité de s'agrandir et de trouver des capitaux, le succès d'une expansion acquise ou les difficultés d'une reconversion, telles sont les principales raisons qui peuvent conduire une entreprise à se donner de nouvelles structures juridiques.
Ainsi, la transformation de la forme de l'entreprise apparaît comme la solution la plus adéquate car; en changeant la forme nous ne faisons que changer les règles qui la régissent.
Reste intacte l'entreprise elle-même.
Blinkverse: A Database of Fast Radio Bursts
Creators: name: Xu, Jiaying
name: Feng, Yi
name: Li, Di
name: Wang, Pei
name: Zhang, Yongkun
name: Xie, Jintao
name: Chen, Huaxi
name: Wang, Han
name: Kang, Zhixuan
name: Hu, Jingjing
name: Zheng, Yun
name: Tsai, Chao-Wei
name: Chen, Xianglei
name: Zhou, Dengke
Publisher: name: arXiv
Year: 2023
Subjects: subject: High Energy Astrophysical Phenomena (astro-ph.HE)
subject: FOS: Physical sciences

description: The volume of research on fast radio bursts (FRBs) observation have been seeing a dramatic growth. To facilitate the systematic analysis of the FRB population, we established a database platform, Blinkverse (https://blinkverse.alkaidos.cn), as a central inventory of FRBs from various observatories and with published properties, particularly dynamic spectra from FAST, CHIME, GBT, Arecibo, etc. Blinkverse thus not only forms a superset of FRBCAT, TNS, and CHIME/FRB, but also provides convenient access to thousands of FRB dynamic spectra from FAST, some of which were not available before. Blinkverse is regularly maintained and will be updated by external users in the future. Data entries of FRBs can be retrieved through parameter searches through FRB location, fluence, etc., and their logical combinations. Interactive visualization was built into the platform. We analyzed the energy distribution, period analysis, and classification of FRBs based on data downloaded from Blinkverse. The energy distributions of repeaters and non-repeaters are found to be distinct from one another.
description: 13 pages, 9 figures
A High-contrast Imaging Algorithm: Optimized Image Rotation and Subtraction
Creators: name: Dou, Jiangpei
name: Ren, Deqing
name: Zhao, Gang
name: Zhang, Xi
name: Chen, Rui
name: Zhu, Yongtian
Publisher: name: arXiv
Year: 2015
Subjects: subject: Instrumentation and Methods for Astrophysics (astro-ph.IM)
subject: Earth and Planetary Astrophysics (astro-ph.EP)
subject: FOS: Physical sciences

description: Image Rotation and Subtraction (IRS) is a high-contrast imaging technique which can be used to suppress the speckles noise and facilitate the direct detection of exoplanets. IRS is different from Angular Differential Imaging (ADI), in which it will subtract a copy of the image with 180 degrees rotated around its PSF center, rather than the subtraction of the median of all of the PSF images. Since the planet itself will be rotated to the other side of the PSF, IRS does not suffer from planet self-subtraction. In this paper, we have introduced an optimization algorithm to IRS (OIRS), which can provide an extra contrast gain at small angular separations. The performance of OIRS has been demonstrated with ADI data. We then made a comparison of the signal to noise ratio (S/N) achieved by algorithms of locally optimized combination of images (LOCI) and OIRS. Finally we found that OIRS algorithm can deliver a better S/N for small angular separations.
description: 18 pages, 8 eps figures, 1 table, accepted for publication in The Astrophysical Journalon on Jan. 10th, 2015
Zinc-finger nuclease-mediated gene correction using single AAV vector transduction and enhancement by Food and Drug Administration-approved drugs
Creators: name: Porteus, M H
name: Samulski, R J
name: Ellis, B L
name: Porter, S N
name: Hirsch, M L
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2013

description: An emerging strategy for the treatment of monogenic diseases uses genetic engineering to precisely correct the mutation(s) at the genome level. Recent advancements in this technology have demonstrated therapeutic levels of gene correction using a zinc-finger nuclease (ZFN)-induced DNA double-strand break in conjunction with an exogenous DNA donor substrate. This strategy requires efficient nucleic acid delivery and among viral vectors, recombinant adeno-associated virus (rAAV) has demonstrated clinical success without pathology. However, a major limitation of rAAV is the small DNA packaging capacity and to date, the use of rAAV for ZFN gene delivery has yet to be reported. Theoretically, an ideal situation is to deliver both ZFNs and the repair substrate in a single vector to avoid inefficient gene targeting and unwanted mutagenesis, both complications of a rAAV co-transduction strategy. Therefore, a rAAV format was generated in which a single polypeptide encodes the ZFN monomers connected by a ribosome skipping 2A peptide and furin cleavage sequence. On the basis of this arrangement, a DNA repair substrate of 750 nucleotides was also included in this vector. Efficient polypeptide processing to discrete ZFNs is demonstrated, as well as the ability of this single vector format to stimulate efficient gene targeting in a human cell line and mouse model derived fibroblasts. Additionally, we increased rAAV-mediated gene correction up to sixfold using a combination of Food and Drug Administration-approved drugs, which act at the level of AAV vector transduction. Collectively, these experiments demonstrate the ability to deliver ZFNs and a repair substrate by a single AAV vector and offer insights for the optimization of rAAV-mediated gene correction using drug therapy.
Do transitive preferences always result in indifferent divisions?
Creators: name: Makowski, Marcin
name: Piotrowski, Edward W.
name: Sładkowski, Jan
Publisher: name: arXiv
Year: 2015
Subjects: subject: Quantum Physics (quant-ph)
subject: FOS: Physical sciences

description: The transitivity of preferences is one of the basic assumptions used in the theory of games and decisions. It is often equated with rationality of choice and is considered useful in building rankings. Intransitive preferences are considered paradoxical and undesirable. This problem is discussed by many social and natural sciences. The paper discusses a simple model of sequential game in which two players in each iteration of the game choose one of the two elements. They make their decisions in different contexts defined by the rules of the game. It appears that the optimal strategy of one of the players can only be intransitive! (the so-called \textsl{relevant intransitive strategies}.) On the other hand, the optimal strategy for the second player can be either transitive or intransitive. A quantum model of the game using pure one-qubit strategies is considered. In this model, an increase in importance of intransitive strategies is observed -- there is a certain course of the game where intransitive strategies are the only optimal strategies for both players. The study of decision-making models using quantum information theory tools may shed some new light on the understanding of mechanisms that drive the formation of types of preferences.
description: 16 pages, 5 figures
Mapping the average AGN accretion rate in the SFR-M* plane for Herschel selected galaxies at 0
Creators: name: Delvecchio, I.
name: Lutz, D.
name: Berta, S.
name: Rosario, D. J.
name: Zamorani, G.
name: Pozzi, F.
name: Gruppioni, C.
name: Vignali, C.
name: Brusa, M.
name: Cimatti, A.
name: Clements, D. L.
name: Cooray, A.
name: Farrah, D.
name: Lanzuisi, G.
name: Oliver, S.
name: Rodighiero, G.
name: Santini, P.
name: Symeonidis, M.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Astrophysics of Galaxies (astro-ph.GA)
subject: FOS: Physical sciences

description: We study the relation of AGN accretion, star formation rate (SFR), and stellar mass (M$_*$) using a sample of $\approx$ 8600 star-forming galaxies up to z=2.5 selected with \textit{Herschel} imaging in the GOODS and COSMOS fields. For each of them we derive SFR and M$_*$, both corrected, when necessary, for emission from an active galactic nucleus (AGN), through the decomposition of their spectral energy distributions (SEDs). About 10 per cent of the sample are detected individually in \textit{Chandra} observations of the fields. For the rest of the sample we stack the X-ray maps to get average X-ray properties. After subtracting the X-ray luminosity expected from star formation and correcting for nuclear obscuration, we derive the average AGN accretion rate for both detected sources and stacks, as a function of M$_{*}$, SFR and redshift. The average accretion rate correlates with SFR and with M$_*$. The dependence on SFR becomes progressively more significant at z$>$0.8. This may suggest that SFR is the original driver of these correlations. We find that average AGN accretion and star formation increase in a similar fashion with offset from the star-forming "main-sequence". Our interpretation is that accretion onto the central black hole and star formation broadly trace each other, irrespective of whether the galaxy is evolving steadily on the main-sequence or bursting.
description: Accepted for publication in MNRAS. 18 pages, 9 figures, 3 tables
Adeno-associated virus inverted terminal repeats stimulate gene editing
Creators: name: Hirsch, M L
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2015

description: Advancements in genome editing have relied on technologies to specifically damage DNA which, in turn, stimulates DNA repair including homologous recombination (HR). As off-target concerns complicate the therapeutic translation of site-specific DNA endonucleases, an alternative strategy to stimulate gene editing based on fragile DNA was investigated. To do this, an episomal gene-editing reporter was generated by a disruptive insertion of the adeno-associated virus (AAV) inverted terminal repeat (ITR) into the egfp gene. Compared with a non-structured DNA control sequence, the ITR induced DNA damage as evidenced by increased gamma-H2AX and Mre11 foci formation. As local DNA damage stimulates HR, ITR-mediated gene editing was investigated using DNA oligonucleotides as repair substrates. The AAV ITR stimulated gene editing >1000-fold in a replication-independent manner and was not biased by the polarity of the repair oligonucleotide. Analysis of additional human DNA sequences demonstrated stimulation of gene editing to varying degrees. In particular, inverted yet not direct, Alu repeats induced gene editing, suggesting a role for DNA structure in the repair event. Collectively, the results demonstrate that inverted DNA repeats stimulate gene editing via double-strand break repair in an episomal context and allude to efficient gene editing of the human chromosome using fragile DNA sequences.
Prediction of adeno-associated virus neutralizing antibody activity for clinical application
Creators: name: Wang, M
name: Li, C
name: McPhee, S
name: Crosby, A
name: Samulski, R J
name: Hastie, E
name: Joshua, G
name: Samulski, J J
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2015

description: Patients with neutralizing antibodies (Nab) against adeno-associated virus (AAV) are usually excluded from treatment with AAV vectors. To develop a standard assay for detecting Nab inhibition activity, we systematically studied current AAV Nab assays in vitro and in vivo. Several factors were found that influence the Nab titers based on the in vitro assay, including: sera volume, AAV dose/cell, cell number and choice of transgenes. When the Nab titer assay was performed in vivo via intramuscular (IM) or systemic administration, a 4-fold increase in sensitivity for measurement of Nab titers was observed compared to an identical in vitro test. To better mimic the clinical setting, after passively transferring human Nabs into mice, blood was collected before systemic injection of AAV vector and used for Nab titer analysis in vitro or via IM injection. The results showed that AAV delivered via IM injection had a similar inhibition pattern to systemic administration. These studies indicate critical parameters necessary for optimizing Nab sensitivity and that an in vivo Nab assay is more sensitive than an in vitro assay for inclusion/exclusion criteria. The variables identified by this study may explain some of the compounding clinical data seen to date with respect to efficiency of AAV transduction in various Phase I clinical trials.
Abelian quotients of triangulated categories
Creators: name: Grimeland, Benedikte
name: Jacobsen, Karin Marie
Publisher: name: arXiv
Year: 2015
Subjects: subject: Representation Theory (math.RT)
subject: FOS: Mathematics

description: We study abelian quotient categories A=T/J, where T is a triangulated category and J is an ideal of T. Under the assumption that the quotient functor is cohomological we show that it is representable and give an explicit description of the functor. We give technical criteria for when a representable functor is a quotient functor, and a criterion for when J gives rise to a cluster-tilting subcategory of T. We show that the quotient functor preserves the AR-structure. As an application we show that if T is a finite 2-Calabi-Yau category, then with very few exceptions J is a cluster-tilting subcategory of T.
description: 20 pages. Minor changes; have stated more explicitly where finiteness is required
LCA Ontology: From ISO 14040 knowledge to EMMO
Creators: name: Paponetti, Ilaria Maria
Publisher: name: Zenodo
Year: 2023
Subjects: ontology
standard
data management
global workshop
ontology commons ecosystem
FAIR

description: Life Cycle Assessment (LCA) is the study of the environmental impact of products taking into account their entire life-span and production chain. This requires gathering data from a variety of heterogeneous sources into a Life Cycle Inventory (LCI). LCI preparation involves the integration of observations and engineering models with reference data and literature results from around the world, from different domains, and at varying levels of granularity. Existing LCA data formats only address syntactic interoperability, thereby often ignoring semantics. The LCA Ontology Methodology aims to overcome this issue.
Forging a future from a failed past? prospects and pitfalls of Somalia’s state-sponsored vigilantism, Ma’awisley
Creators: name: Tahir, Abdifatah Ismael
Publisher: name: figshare
Year: 2024
Subjects: classificationCode: 440606
subject: Political geography
classificationCode: 440804
subject: Defence studies
classificationCode: 440810
subject: Peace studies

description: This paper examines the historical and contemporary dynamics of vigilante mobilizations in Somalia. It shows that these mobilizations have repeatedly failed to achieve the desired outcomes, namely quelling insurgencies. The most recent of these mobilizations began in 2022, when the Hawadle clan, refusing to pay taxes and pledge loyalty to Al-Shabaab, launched a significant offensive against the group. Recognizing a potential advantage, the new government, headed by President Hassan Sheikh Mohamud, who succeeded President Mohamed Abdullahi Farmajo, integrated the clan mobilization into its broader strategy of fighting Al-Shabaab and reclaiming territories in south central Somalia from the group. However, as with previous attempts, this approach proved ineffective. Drawing on interviews and media monitoring conducted between June 2022 and August 2024, the paper argues that these failures result from a fundamental misalignment between the traditional ethos and character of clan-based mobilizations and the external pressures exerted on them by contemporary state-making politics. Furthermore, the paper emphasizes the critical need for a dialogue aimed at facilitating a negotiated solution, which it regards as essential for achieving lasting peace and stability. In so doing, the paper contributes to ongoing calls for negotiation and engagement with Al-Shabaab, despite the considerable challenges inherent in such a process.
Create Models from Frequency Response Analysis
Creators: name: Hubatka, Michael
Publisher: name: Zenodo
Year: 2022

description: This tutorial shows how to convert the data from the Multitone Analysis in PLECS (or other frequency response measurements) to a transfer function, a parametric model. This model will support the compensator design by giving instant information about stability, closed-loop performance, and robustness measures.
description: {"references": ["Ljung, Lennart (2012): System identification. Theory for the user. 2. ed., 14. printing. Upper Saddle River, NJ: Prentice Hall (Prentice-Hall information and system sciences series)", "Isermann, Rolf; M\u00fcnchhof, Marco (2014): Identification of Dynamic Systems. An Introduction with Applications. Aufl. 2011. Berlin: Springer Berlin", "Hrycej, Tomas (2018): Robuste Regelung. Ein Leitfaden f\u00fcr sicherheitskritische Anwendungen. 1. Auflage 2018. Berlin, Heidelberg: Springer Berlin Heidelberg", "Benner, Peter; Grivet-Talocia, Stefano; Quarteroni, Alfio; Rozza, Gianluigi; Schilders, Wil; Silveira, Lu\u00eds Miguel (Hg.) (2021): System- and Data-Driven Methods and Algorithms: De Gruyter"]}
CEP-stable Tunable THz-Emission Originating from Laser-Waveform-Controlled Sub-Cycle Plasma-Electron Bursts
Creators: name: Balčiūnas, T.
name: Lorenc, D.
name: Ivanov, M.
name: Smirnova, O.
name: Zheltikov, A. M.
name: Dietze, D.
name: Darmo, J.
name: Unterrainer, K.
name: Rathje, T.
name: Paulus, G.
name: Baltuška, A.
name: Haessler, S.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Atomic Physics (physics.atom-ph)
subject: Optics (physics.optics)
subject: FOS: Physical sciences

description: We study THz-emission from a plasma driven by an incommensurate-frequency two-colour laser field. A semi-classical transient electron current model is derived from a fully quantum-mechanical description of the emission process in terms of sub-cycle field-ionization followed by continuum-continuum electron transitions. For the experiment, a CEP-locked laser and a near-degenerate optical parametric amplifier are used to produce two-colour pulses that consist of the fundamental and its near-half frequency. By choosing two incommensurate frequencies, the frequency of the CEP-stable THz-emission can be continuously tuned into the mid-IR range. This measured frequency dependence of the THz-emission is found to be consistent with the semi-classical transient electron current model, similar to the Brunel mechanism of harmonic generation.
Capitale sociale e Civic Hacking: forme di partecipazione innovative nell'era dei dati
Creators: name: D'Amico, Adhara
Publisher: name: Zenodo
Year: 2022
Subjects: civic
hacking
civic hacking
social capital
capitale sociale
open data
open government
open
open data sicilia
data
sicilia
civic hacker
hacker
sicily
ODS
Spaghetti Open Data
openness
big data
robert putnam
putnam
coleman

description: Da circa dieci anni le Pubbliche Amministrazioni italiane sono obbligate per legge a condividere e pubblicare sui loro siti web i dati di dominio pubblico da loro prodotti e collezionati; tuttavia, non tutti i dati così pubblicati sono veramente significativi per il cittadino comune, che potrebbe non avere le capacità e le conoscenze per trarre, dagli stessi, informazioni rilevanti e conoscenza. La presa di coscienza di questo gap ha portato alcune persone ad iniziare autonomamente ad occuparsi dell’analisi e della trasformazione di tali dati, per poter offrire un servizio ai cittadini meno pratici: nasce così il Civic Hacking ovvero l'impegno, di alcuni soggetti più o meno specializzati nella comprensione di dati, di utilizzare questi ultimi dati in modo creativo, utile e pratico, animati dalla necessità di rendere più democratica l’informazione e facilitare la partecipazione cittadina alla cosa pubblica. In questa tesi, si vuole: • Introdurre, discutere e prendere in esame l’attivismo civico così costituito, analizzandone le dinamiche tramite lo studio delle attività svolte da una community italiana, Open Data Sicilia; • Capire chi siano i soggetti coinvolti e che background li caratterizzi; • Infine, si vogliono analizzare le prospettive del Civic Hacking dal punto di vista del capitale sociale, le sue potenzialità e l'effettiva capacità di costituirne una fonte per l’individuo e per la società in generale.
Fermi/LAT observations of Dwarf Galaxies highly constrain a Dark Matter Interpretation of Excess Positrons seen in AMS-02, HEAT, and PAMELA
Creators: name: Lopez, Alejandro
name: Savage, Christopher
name: Spolyar, Douglas
name: Adams, Douglas Q.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Cosmology and Nongalactic Astrophysics (astro-ph.CO)
subject: High Energy Physics - Phenomenology (hep-ph)
subject: FOS: Physical sciences

description: It is shown that a Weakly Interacting Massive dark matter Particle (WIMP) interpretation for the positron excess observed in a variety of experiments, HEAT, PAMELA, and AMS-02, is highly constrained by the Fermi/LAT observations of dwarf galaxies. In particular, this paper has focused on the annihilation channels that best fit the current AMS-02 data (Boudaud et al., 2014). The Fermi satellite has surveyed the $γ$-ray sky, and its observations of dwarf satellites are used to place strong bounds on the annihilation of WIMPs into a variety of channels. For the single channel case, we find that dark matter annihilation into {$b\bar{b}$, $e^+e^-$, $μ^+μ^-$, $τ^+τ^-$, 4-$e$, or 4-$τ$} is ruled out as an explanation of the AMS positron excess (here $b$ quarks are a proxy for all quarks, gauge and Higgs bosons). In addition, we find that the Fermi/LAT 2$σ$ upper limits, assuming the best-fit AMS-02 branching ratios, exclude multichannel combinations into $b\bar{b}$ and leptons. The tension between the results might relax if the branching ratios are allowed to deviate from their best-fit values, though a substantial change would be required. Of all the channels we considered, the only viable channel that survives the Fermi/LAT constraint and produces a good fit to the AMS-02 data is annihilation (via a mediator) to 4-$μ$, or mainly to 4-$μ$ in the case of multichannel combinations.
description: 9 pages, 3 figures. v2: removed spurious space in title. v3,v4: added references
scAAVIL-1ra dosing trial in a large animal model and validation of long-term expression with repeat administration for osteoarthritis therapy
Creators: name: Gray, S J
name: Goodrich, L R
name: Khan, N
name: Phillips, J N
name: McIlwraith, C W
name: Samulski, R J
name: Grieger, J C
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2015

description: A gene therapeutic approach to treat osteoarthritis (OA) appears to be on the horizon for millions of people who suffer from this disease. Previously we described optimization of a scAAVIL-1ra gene therapeutic vector and initially tested this in an equine model verifying long-term intrasynovial IL-1ra protein at therapeutic levels. Using this vector, we carried out a dosing trial in six horses to verify protein levels and establish a dose that would express relevant levels of therapeutic protein for extended periods of time (8 months). A novel arthroscopic procedure used to detect green fluorescence protein (GFP) fluorescence intrasynovially confirmed successful transduction of the scAAVGFP vector in both the synovial and cartilage tissues. No evidence of intra-articular toxicity was detected. Immune responses to vector revealed development of neutralizing antibodies (Nabs) within 2 weeks of administration, which persisted for the duration of the study but did not lower protein expression intra-articularly. Re-dosing with a different serotype to attain therapeutic levels of protein confirmed establishment of successful transduction. This is the first study in an equine model to establish a dosing/redosing protocol, as well as examine the Nab response to capsid and supports further clinical investigation to determine the clinical efficacy of scAAVIL-1ra to treat OA.
SPARC (secreted protein acidic and rich in cysteine) knockdown protects mice from acute liver injury by reducing vascular endothelial cell damage
Creators: name: Piccioni, F
name: Atorrasagasti, C
name: Militello, R
name: Fiore, E
name: Corrales, F
name: Mazzolini, G
name: Podhajcer, O
name: Bataller, R
name: García, M G
name: Gidekel, M
name: Aquino, J B
name: Salvatierra, E
name: Alaniz, L
name: Bayo, J
name: Colombo, M I
name: Peixoto, E
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2015

description: Secreted protein, acidic and rich in cysteine (SPARC) is involved in many biological process including liver fibrogenesis, but its role in acute liver damage is unknown. To examine the role of SPARC in acute liver injury, we used SPARC knock-out (SPARC−/−) mice. Two models of acute liver damage were used: concanavalin A (Con A) and the agonistic anti-CD95 antibody Jo2. SPARC expression levels were analyzed in liver samples from patients with acute-on-chronic alcoholic hepatitis (AH). SPARC expression is increased on acute-on-chronic AH patients. Knockdown of SPARC decreased hepatic damage in the two models of liver injury. SPARC−/− mice showed a marked reduction in Con A-induced necroinflammation. Infiltration by CD4+ T cells, expression of tumor necrosis factor-α and interleukin-6 and apoptosis were attenuated in SPARC−/− mice. Sinusoidal endothelial cell monolayer was preserved and was less activated in Con A-treated SPARC−/− mice. SPARC knockdown reduced Con A-induced autophagy of cultured human microvascular endothelial cells (HMEC-1). Hepatic transcriptome analysis revealed several gene networks that may have a role in the attenuated liver damaged found in Con A-treated SPARC−/− mice. SPARC has a significant role in the development of Con A-induced severe liver injury. These results suggest that SPARC could represent a therapeutic target in acute liver injury.
The nature of the [CII] emission in dusty star-forming galaxies from the SPT survey
Creators: name: Gullberg, Bitten
name: De Breuck, Carlos
name: Vieira, Joaquin
name: Weiss, Axel
name: Aguirre, James
name: Aravena, Manuel
name: Béthermin, Matthieu
name: Bradford, C. Matt
name: Bothwell, Matt
name: Carlstrom, John
name: Chapman, Scott
name: Fassnacht, Chris
name: Gonzalez, Anthony
name: Greve, Thomas
name: Hezavah, Yashar
name: Holzapfel, William L.
name: Husband, Kate
name: Ma, Jingzhe
name: Malkan, Matt
name: Marrone, Dan
name: Menten, Karl
name: Murphy, Eric
name: Reichardt, Christian
name: Spilker, Justin
name: Stark, Anthony
name: Strandet, Maria
name: Welikala, Niraj
Publisher: name: arXiv
Year: 2015
Subjects: subject: Astrophysics of Galaxies (astro-ph.GA)
subject: FOS: Physical sciences

description: We present [CII] observations of 20 strongly lensed dusty star forming galaxies at 2.1 < z < 5.7 using APEX and Herschel. The sources were selected on their 1.4 mm flux (S_1.4mm > 20 mJy) from the South Pole Telescope survey, with far-infrared (FIR) luminosities determined from extensive photometric data. The [CII] line is robustly detected in 17 sources, all but one being spectrally resolved. Eleven out of 20 sources observed in [CII] also have low-J CO detections from ATCA. A comparison with mid- and high-J CO lines from ALMA reveals consistent [CII] and CO velocity profiles, suggesting that there is little differential lensing between these species. The [CII], low-J CO and FIR data allow us to constrain the properties of the interstellar medium. We find [CII] to CO(1-0) luminosity ratios in the SPT sample of 5200 +- 1800, with significantly less scatter than in other samples. This line ratio can be best described by a medium of [CII] and CO emitting gas with a higher [CII] than CO excitation temperature, high CO optical depth tau_CO >> 1, and low to moderate [CII] optical depth tau_CII ~< 1. The geometric structure of photodissociation regions allows for such conditions.
description: 19 Pages, 12 figures, accepted for publication in MNRAS
17th RDA Plenary Poster Presentation: re3data - Discovering FAIR-enabling Repositories
Creators: name: Ulrich, Robert
name: Weisweiler, Nina Leonie
name: Wimalaratne, Sarala
name: Witt, Michael
Publisher: name: Forschungzentrum Jülich
Year: 2021
Subjects: RDA Plenary 17
Research Data Repositories
re3data
FAIR Research Data
DataCite Commons
Repository Finder
FAIRsFAIR Project

description: doi:10.5281/ZENODO.4705209
description: The poster gives an overview of the transition of the Repository Finder [1] into DataCite Commons [2] within the FAIRsFAIR project to enable researchers identify FAIR enabling repositories [3] to either deposit their research data or find research output, organizations or researchers related to the repository. Using re3data as an extensive source for research data repositories the poster will also provide insights on the implementation of the related changes to the metadata schema and the API of re3data, being initial work towards greater FAIRness.These activities are carried out within the framework of the re3data COREF project [4]. As there are still many open questions like community agreed PIDs for repositories, final implementation of a “FAIR certification”, or collaboration with other catalogues and registries describing repositories, the poster session shall foster discussions about future works on these topics and evaluate reasonable developments to be integrated in the time to come. [1] https://repositoryfinder.datacite.org/ [2] https://commons.datacite.org/ [3] https://doi.org/10.5281/zenodo.4590298 [4] https://coref.project.re3data.org/project