title: ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ ÑÐ¸Ð·Ð¾Ð±Ð¸Ð¾ÑÐ°Ð³Ð¾Ð² Ð¸ ÐºÐ»ÑÐ±ÐµÐ½ÑÐºÐ¾Ð²ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ S. meliloti Ð² Ð¿ÑÐ¸ÑÐ¾Ð´Ð½ÑÑ Ð¿Ð¾Ð¿ÑÐ»ÑÑÐ¸ÑÑ Ð¡ÐµÐ²ÐµÑÐ¾-Ð·Ð°Ð¿Ð°Ð´Ð½Ð¾Ð³Ð¾ ÑÐµÐ³Ð¸Ð¾Ð½Ð° Ð Ð¤
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Investigation of the interaction between rhizobiophages and root nodulated bacteria of S. meliloti in native populations of the North-West region of the RF
Creators: name: ÐÐ¾ÑÐ±ÑÐ½Ð¾Ð²Ð°, ÐÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: ÑÐ¸Ð·Ð¾Ð±Ð¸Ð¾ÑÐ°Ð³Ð¸
ÐºÐ»ÑÐ±ÐµÐ½ÑÐºÐ¾Ð²ÑÐµ Ð±Ð°ÐºÑÐµÑÐ¸Ð¸
Ð»Ð¸ÑÐ¸ÑÐµÑÐºÐ°Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÑÐ¸Ð·Ð¾Ð±Ð¸Ð¾ÑÐ°Ð³Ð¾Ð²
Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° ÑÐ¸Ð·Ð¾Ð±Ð¸Ð¾ÑÐ°Ð³Ð¾Ð²
rizobiophages
nodule bacteria
rizobiophage lytic activity
rizobiophages biological properties

description: ÐÐ°Ð´Ð°ÑÐ¸, ÑÐµÑÐ°ÐµÐ¼ÑÐµ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: 1. Ð¾ÑÐ²Ð¾ÐµÐ½Ð¸Ðµ Ð¸ Ð°Ð´Ð°Ð¿ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² ÑÐ°Ð±Ð¾ÑÑ Ñ ÑÐ¸Ð·Ð¾Ð±Ð¸Ð¾ÑÐ°Ð³Ð°Ð¼Ð¸; 2. Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ðµ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ ÑÐ¸Ð·Ð¾Ð±Ð¸Ð¾ÑÐ°Ð³Ð¾Ð² Ð¸Ð· Ð³Ð¾ÑÐ¾Ð´ÑÐºÐ¸Ñ Ð¿Ð¾ÑÐ² Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¼ÐµÑÐ¾Ð´Ð° Ð¾Ð±Ð¾Ð³Ð°ÑÐµÐ½Ð¸Ñ Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ðµ ÑÐ¸ÑÑÑÑ ÐºÑÐ»ÑÑÑÑ ÑÐ°Ð³Ð¾Ð² Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¼ÐµÑÐ¾Ð´Ð° Ð´Ð²ÑÑÐ»Ð¾Ð¹Ð½Ð¾Ð³Ð¾ Ð°Ð³Ð°ÑÐ° Ð¿Ð¾ ÐÐ´Ð°Ð¼ÑÑ; 3. Ð¾ÑÐµÐ½ÐºÐ° Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ ÑÐ²Ð¾Ð¹ÑÑÐ² Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ ÑÐ¸Ð·Ð¾Ð±Ð¸Ð¾ÑÐ°Ð³Ð¾Ð² (Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ñ Ð½ÐµÐ³Ð°ÑÐ¸Ð²Ð½ÑÑ ÐºÐ¾Ð»Ð¾Ð½Ð¸Ð¹, ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÐ½Ð°Ñ ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾ÑÑÑ, Ð¾ÑÐµÐ½ÐºÐ° ÑÐ¿ÐµÐºÑÑÐ° Ð»Ð¸ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸, ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¾Ð½Ð½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð· ÑÐ°Ð³Ð¾Ð²Ð¾Ð¹ ÐÐÐ); 4. Ð¾ÑÐ²Ð¾ÐµÐ½Ð¸Ðµ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ ÑÑÐ°Ð¼Ð¼Ð¾Ð² ÐºÐ»ÑÐ±ÐµÐ½ÑÐºÐ¾Ð²ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹; Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ ÑÑÐ°Ð¼Ð¼Ð¾Ð² Ð¸Ð· Ð³Ð¾ÑÐ¾Ð´ÑÐºÐ¸Ñ Ð¿Ð¾ÑÐ² Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ plantâtrapping (Ð¸Ð· ÐºÐ»ÑÐ±ÐµÐ½ÑÐºÐ¾Ð², Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð½ÑÑ Ð½Ð° ÐºÐ¾ÑÐ½ÑÑ ÑÐ°ÑÑÐµÐ½Ð¸ÑâÑÐ¾Ð·ÑÐ¸Ð½Ð° Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ Ð¼Ð¸ÐºÑÐ¾Ð²ÐµÐ³ÐµÑÐ°ÑÐ¸Ð¾Ð½Ð½ÑÑ Ð¾Ð¿ÑÑÐ¾Ð²) Ð¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ðµ Ð²Ð¸Ð´Ð¾Ð²Ð¾Ð¹ Ð¸Ð´ÐµÐ½ÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ ÑÑÐ°Ð¼Ð¼Ð¾Ð² ÐºÐ»ÑÐ±ÐµÐ½ÑÐºÐ¾Ð²ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° ÐÐ¦Ð âÐÐÐ Ð¤ ÑÑÐ°Ð³Ð¼ÐµÐ½ÑÐ° Ð³ÐµÐ½Ð° 16S ÑÐÐÐ; 5. Ð¾ÑÐµÐ½ÐºÐ° ÑÐ°Ð³Ð¾Ð²Ð¾âÐ¼Ð¸ÐºÑÐ¾Ð±Ð½ÑÑ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ð¹ Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ ÑÐ¾Ð²Ð¼ÐµÑÑÐ½Ð¾Ð³Ð¾ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð² Ð¶Ð¸Ð´ÐºÐ¾Ð¹ ÐºÑÐ»ÑÑÑÑÐµ Ð¸ Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ Ð¼Ð¸ÐºÑÐ¾Ð²ÐµÐ³ÐµÑÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð³Ð¾ Ð¾Ð¿ÑÑÐ°. ÐÐ±ÑÐµÐºÑÐ°Ð¼Ð¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐ²Ð»ÑÐ»Ð¸ÑÑ ÑÐ¸Ð·Ð¾Ð±Ð¸Ð¾ÑÐ°Ð³Ð¸ Ð¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð¸ Ð²Ð¸Ð´Ð° Sinorhizobium meliloti. Ð Ð°Ð±Ð¾ÑÐ° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ Ð³ÐµÐ½ÐµÑÐ¸ÐºÐ¸ Ð¸ ÑÐµÐ»ÐµÐºÑÐ¸Ð¸ Ð¼Ð¸ÐºÑÐ¾Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð¾Ð² Ð¤ÐÐÐÐ£ ÐÐÐÐÐ¡Ð¥Ð. Ð Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¾ ÑÑÐ¸ Ð½Ð¾Ð²ÑÑ ÑÐ¸Ð·Ð¾Ð±Ð¸Ð¾ÑÐ°Ð³Ð° Ð¸ Ð¿ÑÑÐ½Ð°Ð´ÑÐ°ÑÑ Ð¿ÑÐ¸ÑÐ¾Ð´Ð½ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð² ÐºÐ»ÑÐ±ÐµÐ½ÑÐºÐ¾Ð²ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ Ð´Ð²ÑÑ Ð±Ð»Ð¸Ð·ÐºÐ¾ÑÐ¾Ð´ÑÑÐ²ÐµÐ½Ð½ÑÑ Ð²Ð¸Ð´Ð¾Ð² Sinorhizobium meliloti Ð¸ Sinorhizobium medicae Ð¸Ð· Ð³Ð¾ÑÐ¾Ð´ÑÐºÐ¾Ð¹ ÑÑÐµÐ´Ñ. ÐÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð°Â  Ð¾ÑÐµÐ½ÐºÐ° Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ ÑÐ²Ð¾Ð¹ÑÑÐ² ÐºÑÐ»ÑÑÑÑ ÑÐ¸Ð·Ð¾Ð±Ð¸Ð¾ÑÐ°Ð³Ð¾Ð² Ð¸ Ð¾ÑÐµÐ½ÐµÐ½Ð° Ð¸Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð² ÑÐ°Ð³Ð¾Ð²Ð¾-Ð¼Ð¸ÐºÑÐ¾Ð±Ð½ÑÑ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸ÑÑ Ð² ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ ÑÑÐ»Ð¾Ð²Ð¸ÑÑ. Ð£ÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¾, ÑÑÐ¾ ÑÐ¸Ð·Ð¾Ð±Ð¸Ð¾ÑÐ°Ð³Ð¸ ÑÑÑÐµÑÑÐ²ÐµÐ½Ð½Ð¾ ÑÐ°Ð·Ð»Ð¸ÑÐ°ÑÑÑÑ Ð¿Ð¾ ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐ½Ð¾ÑÑÐ¸ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ ÐºÐ°Ðº ÑÐ¾ ÑÑÐ°Ð¼Ð¼Ð°Ð¼Ð¸ ÑÐ¸Ð·Ð¾Ð±Ð¸Ð¹ Ð¾Ð´Ð½Ð¾Ð³Ð¾ Ð²Ð¸Ð´Ð°, ÑÐ°Ðº Ð¸ Ð¿Ð¾ Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ñ Ð² Ð±Ð»Ð¸Ð·ÐºÐ¾ÑÐ¾Ð´ÑÑÐ²ÐµÐ½Ð½ÑÐ¼ Ð²Ð¸Ð´Ð°Ð¼ ÑÐ¸Ð·Ð¾Ð±Ð¸Ð¹, Ð¸ Ð¼Ð¾Ð³ÑÑ Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÑÑ Ð´Ð¾ÑÑÐ¾Ð²ÐµÑÐ½Ð¾Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð½Ð° Ð¿ÑÐ¾ÑÐµÑÑ ÐºÐ»ÑÐ±ÐµÐ½ÑÐºÐ¾Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ñ. ÐÑÐ¸ Ð½Ð°Ð¿Ð¸ÑÐ°Ð½Ð¸Ð¸ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±Ð·Ð¾ÑÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ: eLIBRARY.RU, Pubmed, Scopus. Ð¡ÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÑÑ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»Ð¸ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼: Microsoft Excel, PAST.
description: The tasks carried out in the course of the study: 1. learn and practice the techniques of dealing with rhizobiophage; 2. isolation of rhizobiophages from soil samples using the enrichment protocol and obtaining pure phage cultures by the Adams double-layer agar method; 3. evaluation of biological properties of the obtained rhizobiophages (morphology of negative colonies, temperature stability, evaluation of phage lytic activity, restriction analysis of phage DNA); 4. learn and practice methods for isolation of nodule bacteria strains; isolation of strains from soil samples by plant-trapping method (from nodules formed on the roots of the host plant under conditions of microvegetation experiment) and conductingÂ  species identification of nodule bacteria strains by PCR-RFLP analysis of 16S rDNA gene fragment; 5. evaluation of phage-microbial interactions under conditions of their co-cultivation in liquid medium and under conditions of microvegetation experiment with plants. The objects of research were rhizobiophages and bacteria of Sinorhizobium meliloti species. The work was carried out in the laboratory of genetics and selection of microorganisms of All-Russia Research Institute of Agricultural Microbiology. In this work three new rhizobiophages and fifteen native strains of rhizobia of the two closely related species Sinorhizobium meliloti Ð¸ SinorhizobiumÂ  medicae were obtained from urban ecosystem. The biological properties of the isolated bacteriophages and their activity in phage-microbe interactions under different conditions were evaluated. The study found that rhizobiophages differ significantly in the specificity of interaction with rhizobia strains of the same species as well as against rhizobia of closely related species and can have a reliable effect on the process of nodulation. When writing the literature review, the following databases were used: eLIBRARY.RU, Pubmed, Scopus. Statistical processing of the experimental data was carried out using the software: Microsoft Excel, PAST.