Additional file 1 of Modifications in steroid and triterpenoid metabolism in Calendula officinalis plants and hairy root culture in response to chitosan treatment
Creators: name: Rogowska, Agata
name: Pączkowski, Cezary
name: Szakiel, Anna
Publisher: name: figshare
Year: 2023
Subjects: subject: Plant Biology
subject: FOS: Biological sciences

description: Additional file 1: Table S1. GC-MS data (retention times and characteristic ions of mass spectra) of identified steroids and triterpenoids. Table S2. Effect of chitosan treatment on steroids content in hairy roots tissue. Data which do not share a common letter are significantly different. Capital letters indicate significant difference in time between plants from the same treatment, lowercase indicate difference between treatments within certain time point. Table S3. Analysis of the interaction of treatment and time on steroid content in hairy roots tissue performed by two-way ANOVA. Table S4. Effect of chitosan treatment on sterol esters content in hairy roots tissue. Data which do not share a common letter are significantly different. Capital letters indicate significant difference in time between plants from the same treatment, lowercase indicate difference between treatments within certain time point. Table S5. Effect of chitosan treatment on sterol glycosides content in hairy roots tissue. Data which do not share a common letter are significantly different. Capital letters indicate significant difference in time between plants from the same treatment, lowercase indicate difference between treatments within certain time point. Table S6. Analysis of the interaction of treatment and time on sterol esters and sterol glycosides content in hairy roots tissue performed by two-way ANOVA. Table S7. Effect of chitosan treatment on neutral terpenoids content in hairy roots tissue. Data which do not share a common letter are significantly different. Capital letters indicate significant difference in time between plants from the same treatment, lowercase indicate difference between treatments within certain time point. Table S8. Analysis of the interaction of treatment and time on neutral triterpenoids (amyrins) content in hairy roots tissue performed by two-way ANOVA. Table S9. Effect of chitosan treatment on free oleanolic acid (OA) and its methyl ester (Met OA) content in hairy roots tissue. Data which do not share a common letter are significantly different. Capital letters indicate significant difference in time between plants from the same treatment, lowercase indicate difference between treatments within certain time point. Table S10. Analysis of the interaction of treatment and time on free oleanolic acid (OA) and its methyl ester (Met OA) content in hairy roots tissue performed by two-way ANOVA. Table S11. Effect of chitosan treatment on oleanolic acid saponins (OA) content in hairy roots tissue. Data which do not share a common letter are significantly different. Capital letters indicate significant difference in time between plants from the same treatment, lowercase indicate difference between treatments within certain time point. Table S12. Analysis of the interaction of treatment and time on oleanolic acid (OA) saponins content in hairy roots tissue performed by two-way ANOVA. Table S13. Effect of chitosan treatment on oleanolic acid saponins (OA) released to the culture medium. Data which do not share a common letter are significantly different. Capital letters indicate significant difference in time between plants from the same treatment, lowercase indicate difference between treatments within certain time point. Table S14. Analysis of the interaction of treatment and time on oleanolic acid (OA) saponins released to the medium performed by two-way ANOVA. Table S15. Content of free sterols, neutral triterpenoids and triterpenoid acids in C. officinalis roots. Data which do not share a common letter are significantly different. Capital letters indicate significant difference in time between plants from the same treatment, lowercase indicate difference between treatments within certain time point. Table S16. Analysis of the interaction of treatment and time on free sterols content in C. officinalis roots performed by two-way ANOVA. Table S17. Analysis of the interaction of treatment and time on neutral triterpenoids (amyrins and friedooleanans) content in C. officinalis roots performed by two-way ANOVA. Table S18. Analysis of the interaction of treatment and time on triterpenoid acids content in C. officinalis roots performed by two-way ANOVA. Table S19. Content of sterols conjugated in sterol esters and sterol glycosides in C. officinalis roots. Data which do not share a common letter are significantly different. Capital letters indicate significant difference in time between plants from the same treatment, lowercase indicate difference between treatments within certain time point. Table S20. Analysis of the interaction of treatment and time on sterol esters and sterol glycosides content in C. officinalis roots performed by two-way ANOVA. Table S21. Content of free sterols, neutral triterpenoids and triterpenoid acids in C. officinalis shoots. Data which do not share a common letter are significantly different. Capital letters indicate significant difference in time between plants from the same treatment, lowercase indicate difference between treatments within certain time point. Table S22. Analysis of the interaction of treatment and time on free sterols content in C. officinalis shoots performed by two-way ANOVA. Table S23. Analysis of the interaction of treatment and time on neutral triterpenoids (amyrins) content in C. officinalis shoots performed by two-way ANOVA. Table S24. Analysis of the interaction of treatment and time on triterpenoid acids content in C. officinalis shoots performed by two-way ANOVA. Table S25. Content of sterols conjugated in sterol esters and sterol glycosides in C. officinalis shoots. Data which do not share a common letter are significantly different. Capital letters indicate significant difference in time between plants from the same treatment, lowercase indicate difference between treatments within certain time point. Table S26. Analysis of the interaction of treatment and time on sterol esters and sterol glycosides content in C. officinalis shoots performed by two-way ANOVA. Table S27. Content of oleanolic acid saponins (OA) in C. officinalis roots and shoots. Data which do not share a common letter are significantly different. Capital letters indicate significant difference in time between plants from the same treatment, lowercase indicate difference between treatments within certain time point. Table S28. Analysis of the interaction of treatment and time on oleanolic acid (OA) saponins content in roots and shoots of C. officinalis performed by two-way ANOVA. Table S29. Content of free sterols, neutral triterpenoids and triterpenoid acids in C. officinalis inflorescences. Data which do not share a common letter are significantly different. Table S30. Analysis of the chitosan treatment on free sterols content in C. officinalis inflorescences performed by T- student test. Table S31. Analysis of the chitosan treatment on neutral triterpenoids content in C. officinalis inflorescences performed by T- student test. Table S32. Analysis of the chitosan treatment on free oleanolic acid (OA) content in C. officinalis inflorescences performed by T- student test. Table S33. Content of sterols conjugated in sterol glycosides in C. officinalis inflorescences. Data which do not share a common letter are significantly different. Table S34. Analysis of the chitosan treatment on sterol glycosides content in C. officinalis inflorescences performed by T- student test. Table S35. Content of oleanolic acid (OA) saponins in C. officinalis inflorescences. Data which do not share a common letter are significantly different. Table S36. Analysis of the chitosan treatment on oleanolic acid (OA) saponins content in C. officinalis inflorescences performed by T- student test. Table S37. Basic physical and chemical charactrerization of universal soil “Athena” including: pH, salinity, concentration of nitrogen (N), potassium oxide (K2O) and phosphates (P2O5).