cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Results From a Randomized Trial of Intensive Glucose Management Using CGM Versus Usual Care in Hospitalized Adults With Type 2 Diabetes: The TIGHT Study.
Creators: name: Monye, Joseph O.
name: Gligorijevic, Nikola
name: Davis, Georgia M.
name: Goley, April
name: Choksi, Palak
name: Raghinaru, Dan
name: Seggelke, Stacey
name: Alexander, Bonnie
name: Kollman, Craig
name: Vinh, Jennifer
name: Spanakis, Elias K.
name: Khakpour, Dori
name: Jones, Morgan S.
name: Huang, Michelle
name: Diner, Jamie
name: Baran, Jesica D.
name: TIGHT RCT Study Group
name: Spanbauer, Charles
name: Sibayan, Judy
name: Umpierrez, Guillermo E.
name: Armstrong, Chase
name: Beck, Roy W.
name: Hirsch, Irl B.
name: Ferrara, Wanda
name: Draznin, Boris
name: Kershaw, Erin E.
name: Matson, Brooke C.
name: Low Wang, Cecilia C.
name: Reynolds, Shari L.
name: Kass, Alex
name: Choe, Monica Y.
name: Adair, Whitney B.
name: Gilani, Faryal
name: Bais, Rajlaxmi
name: Churchill, Jade
name: Garg, Avni
name: Singh, Lakshmi G.
name: Pasquel, Francisco J.
name: Moazzami, Bobak
name: Ullal, Jagdeesh
name: Vellanki, Priyathama
name: Klein, Klara R.
name: Chao, Jing H.
name: Buse, John B.
name: Scott, William
name: Zabala, Zohyra E.
name: Fulghum, Karla
Publisher: name: American Diabetes Association
Year: 2024

description:
OBJECTIVE: To evaluate whether continuous glucose monitoring (CGM) could assist providers in intensifying glycemic management in hospitalized patients with type 2 diabetes. RESEARCH DESIGN AND METHODS: At six academic hospitals, adults with type 2 diabetes hospitalized in a non-intensive care setting were randomly assigned to either standard therapy with glucose target 140-180 mg/dL (standard group) or intensive therapy with glucose target 90-130 mg/dL guided by CGM (intensive group). The primary outcome was mean glucose measured with CGM (blinded in standard group), and the key secondary outcome was CGM glucose <54 mg/dL. RESULTS: For the 110 participants included in the primary analysis, mean ± SD age was 61 ± 12 years and mean HbA1c was 8.9 ± 2.3% (73.8 ± 1.6 mmol/mol). During the study, CGM-measured mean glucose was 170 mg/dL for the intensive group (n = 60) vs. 175 mg/dL for the standard group (n = 50; risk-adjusted difference -7 mg/dL, 95% CI -19 to 5; P = 0.25). Only 7% of the intensive group achieved the mean glucose target range of 90-130 mg/dL. CGM readings <54 mg/dL were infrequent (0.2% for intensive and 0.4% for standard; adjusted treatment group difference -0.1%, 95% CI -0.6 to 0.3). One severe hypoglycemia event occurred in the standard group. CONCLUSIONS: The study's glucose management approach using CGM did not improve glucose levels compared with standard glucose management in the non-intensive care unit hospital setting. A glucose target of 90-130 mg/dL may not be realistic in the current environment of insulin management in the hospital.
Human metabolic chambers reveal a coordinated metabolic-physiologic response to nutrition
Creators: name: Below, Jennifer E.
name: Krakoff, Jonathan
name: Clish, Clary B.
name: Piaggi, Paolo
name: Freedman, Jane
name: Perry, Andrew S.
name: North, Kari E.
name: Huang, Shi
name: Shah, Ravi V.
name: Murthy, Venkatesh L.
name: Nayor, Matthew
Publisher: name: American Society for Clinical Investigation
Year: 2024

description:
Human studies linking metabolism with organism-wide physiologic function have been challenged by confounding, adherence, and precisionHere, we united physiologic and molecular phenotypes of metabolism during controlled dietary intervention to understand integrated metabolic-physiologic responses to nutrition. In an inpatient study of individuals who underwent serial 24-hour metabolic chamber experiments (indirect calorimetry) and metabolite profiling, we mapped a human metabolome onto substrate oxidation rates and energy expenditure across up to 7 dietary conditions (energy balance, fasting, multiple 200% caloric excess overfeeding of varying fat, protein, and carbohydrate composition). Diets exhibiting greater fat oxidation (e.g., fasting, high-fat) were associated with changes in metabolites within pathways of mitochondrial β-oxidation, ketogenesis, adipose tissue fatty acid liberation, and/or multiple anapleurotic substrates for tricarboxylic acid cycle flux, with inverse associations for diets with greater carbohydrate availability. Changes in each of these metabolite classes were strongly related to 24-hour respiratory quotient (RQ) and substrate oxidation rates (e.g., acylcarnitines related to lower 24-hour RQ and higher 24-hour lipid oxidation), underscoring links between substrate availability, physiology, and metabolism in humans. Physiologic responses to diet determined by gold-standard human metabolic chambers are strongly coordinated with biologically consistent, interconnected metabolic pathways encoded in the metabolome.
Impact of Social Determinants of Health on Melanoma Nodal Surveillance in a Multi-institutional Cohort
Creators: name: Archer, Dion
name: Berman, Russell S.
name: Lee, Ann Y.
name: Pham, Veronica
name: Ollila, David W.
name: Rhodin, Kristen E.
name: Hieken, Tina J.
name: Winchester, Sydney
name: Kottschade, Lisa A.
name: McDonald, Hannah G.
name: Sabel, Michael S.
name: Zager, Jonathan S.
name: Broman, Kristy K.
name: Cintolo-Gonzalez, Jessica A.
name: Beasley, Georgia M.
name: Elleson, Kelly M.
name: DePalo, Danielle K.
name: Montgomery, Kelsey B.
name: Burke, Erin E.
name: Dugan, Michelle M.
name: McLeod, M. Chandler
Publisher: name: Springer Nature
Year: 2024

description:
Background Nodal surveillance (NS) has overtaken completion lymphadenectomy as the preferred management for sentinel node-positive (SLN+) melanoma, but requires frequent exams and nodal ultrasound (US). Social determinants of health (SDoH) may affect US adherence in real-world populations, and evaluation of these potential impacts is needed. Methods Adults with SLN+ melanoma diagnosed from July 2017 to December 2019 who received NS at nine cancer centers were identified retrospectively. Exposures included insurance status, travel distance, and Centers for Disease Control and Prevention (CDC) Social Vulnerability Index (SVI), a validated measure of area-level SDoH, indicated as 0 (low) to 1 (high) vulnerability. The primary outcome was US adherence (≥ 1 study per 6-month follow-up interval). The secondary outcomes were combined-modality adherence [US, computed tomography (CT), or positron emission tomography (PET)] and loss to follow-up (LTFU). Bivariate analyses and mixed-effects multivariable logistic regression were performed. Results Most of the 519 patients were male (57%), non-Hispanic white (94.4%), and insured privately (45.3%) or by Medicare (43.5%). The median travel distance was 63.3 miles (interquartile range [IQR], 31.2–111.0 miles), and the median SVI was 0.426 (IQR, 0.253–0.610). The surveillance adherence rates were 41.6% for US and 75.1% for combined modalities. No significant differences in US adherence were observed based on sociodemographic covariates in regression analysis. Medicaid (odds ratio [OR], 3.12; p = 0.02) and uninsured (OR 4.48; p = 0.01) patients had increased likelihood of LTFU. Conclusions Less than half of the patients in this multicenter cohort achieved US adherence, although the rates improved with combined modalities. Medicaid or non-insurance were social risk factors for LTFU. Optimizing surveillance practices for socially vulnerable groups will be crucial for the ongoing real-world implementation of NS.
Contrasting pathogen prevalence between tick and dog populations at Chornobyl
Creators: name: Betz, Jennifer A.
name: Warren, Madeline E.
name: Mousseau, Timothy A.
name: Kleiman, Norman J.
name: Breen, Matthew
name: Qurollo, Barbara A.
name: Thomas, Rachael
name: Dillon, Megan N.
Publisher: name: Springer Nature
Year: 2024

description:
Background The 1986 disaster at the Chornobyl Nuclear Power Plant released massive amounts of radioactive material into the local environment. In addition to radiation, remediation efforts and abandonment of military-industrial complexes contributed to contamination with heavy metals, organics, pesticides and other toxic chemicals. Numerous studies have evaluated the effects of this contamination on the local ecology. However, few studies have reported the effect of this contamination on vector-borne pathogens and their hosts. In this manuscript, we characterize tick-borne pathogen presence at two sample locations within the Chornobyl Exclusion Zone, one at the Nuclear Power Plant (NPP) and another 16 km away in Chornobyl City (CC). Methods Ticks and whole-blood samples were collected from free-breeding dogs captured at the NPP and CC. Endpoint PCR and quantitative PCR were used to identify tick species and to assess the presence of specific tick-borne pathogens, including Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, Babesia spp., Bartonella spp., Francisella tularensis and general Anaplasmataceae. A droplet digital PCR assay was developed for Babesia canis and A. phagocytophilum to evaluate their presence in dogs from the two populations. Pathogen prevalences between the two sample populations were compared by calculating Z-scores. Results Ticks were identified as Ixodes ricinus (n = 102) and Dermacentor reticulatus (n = 4). Overall, 56.9% of I. ricinus ticks were positive for at least one pathogen. A significantly higher prevalence of A. phagocytophilum and B. burgdorferi was found in ticks at the NPP (44.0% and 42.0%, respectively) compared to CC (23.1% and 19.2%, respectively). Babesia spp. (including B. canis and B. caballi) were detected in 8.8% ticks at similar proportions for both populations. Interestingly, we found a significantly lower level of A. phagocytophilum in dogs at the NPP (1.8%) than in dogs at CC (11.7%). In total, 24.3% of dogs were positive for B. canis, evenly distributed across the two populations. Conclusions The results of this study show contrasting pathogen prevalence in both ticks and dogs at the NPP and CC, which may reflect the differential exposures at the two locations. This work adds an important new component to our understanding of the consequences of prolonged exposure to environmental contamination on the wildlife and ecology within the Chornobyl Exclusion Zone.
Generative adversarial networks accurately reconstruct pan-cancer histology from pathologic, genomic, and radiographic latent features
Creators: name: Fan, Cheng
name: Zhang, Qianchen
name: Dolezal, James
name: Pearson, Alexander T
name: Feiger, Brad
name: Peterson, Joseph
name: Kochanny, Sara
name: Cole, Kimberly
name: Hieromnimon, Hanna M
name: Ramesh, Siddhi
name: Sullivan, Megan
name: Vickery, Jasmine
name: Howard, Frederick M
name: Khramtsova, Galina
name: Perou, Charles M
Publisher: name: American Association for the Advancement of Science (AAAS)
Year: 2024

description:
Artificial intelligence models have been increasingly used in the analysis of tumor histology to perform tasks ranging from routine classification to identification of molecular features. These approaches distill cancer histologic images into high-level features, which are used in predictions, but understanding the biologic meaning of such features remains challenging. We present and validate a custom generative adversarial network-HistoXGAN-capable of reconstructing representative histology using feature vectors produced by common feature extractors. We evaluate HistoXGAN across 29 cancer subtypes and demonstrate that reconstructed images retain information regarding tumor grade, histologic subtype, and gene expression patterns. We leverage HistoXGAN to illustrate the underlying histologic features for deep learning models for actionable mutations, identify model reliance on histologic batch effect in predictions, and demonstrate accurate reconstruction of tumor histology from radiographic imaging for a "virtual biopsy."
Community-engaged strategies to improve sexual health services for adults aged 45 and above in the United Kingdom: a qualitative data analysis
Creators: name: Sakuma, Yoshiko
name: Tucker, Joseph D
name: Terris-Prestholt, Fern
name: Nunez, Michel
name: Wu, Dan
name: Kpokiri, Eneyi E
name: Pan, Stephen W
name: Day, Suzanne
name: Ong, Jason J
name: Conyers, Hayley
name: Shakespeare, Tom
Publisher: name: CSIRO Publishing
Year: 2024

description:
Background Sexual health is an essential component of health and well-being across the life course. However, sexual health research often focuses on young adults and excludes those aged 45years and older. We organized a national crowdsourcing open call and co-creation events to identify recommendations to improve sexual health service provision for middle-aged and older adults in the United Kingdom (UK). Methods We conducted a crowdsourcing open call and seven co-creation events consisting of workshop-style meetings and one-to-one in-depth interviews. Open call submissions and qualitative data from the co-creation events were analyzed using a thematic approach. A social-ecological framework was used to code deductively, but new codes were allowed to emerge. Thematic categories were organized to describe factors influencing the accessibility and inclusivity of sexual health services for middle-aged and older adults. Results We received 22 submissions in total; of those, 35% of participants reported a disability, 40% of individuals were aged 45-65years, and 6% of submissions came from individuals that identified as gay/lesbian. Five key themes highlighted that improving sexual health services for adults aged 45years and over requires a multi-leveled approach: increase sexual health education, enhance patient and provider relationships, utilize community-led sexual health promotion efforts and delivery of reliable sexual health information, improve inclusive sexual health services, and break down sexual health taboos against adults aged 45+ years. Conclusions Our data suggest that middle-aged and older adults can co-create compelling strategies to enhance sexual health services for middle-aged and older adults in the UK. Further implementation research is needed to pilot these strategies.
Establishment of a Brain Tumor Consortium of Africa: Advancing Collaborative Research and Advocacy for Brain Tumors in Africa
Creators: name: Jenkins, Robert B
name: Brown, Desmond A
name: Yevudza, W Elorm
name: Tiamiyu, Liadi
name: Gankpe, Gbetoho Fortuné
name: Odukoya, Lateef A
name: Edrick, Elias
name: Huse, Jason
name: Akinjo, Andrea O
name: Higgins, Dominique
name: Magambo, Margreth
name: Magoha, Michael
name: Badmos, Kabir B
name: Bankole, Olufemi
name: Darko, Kwadwo
name: Ekhator, Chukwuyem
name: Etame, Arnold B
name: Zerd, Francis
name: Katasi, Victoria M
name: Ngaiza, Advera
name: Llewellyn, Henry
name: Schroeder, Kristin
name: Barrie, Umaru
name: Ghomsi, Nathalie C
name: Eckel-Passow, Jeanette E
name: Fonkem, Ekokobe
name: Kabare, Gloria
name: Balogun, James
name: Nyalundja, Arsene D
name: Petershie, Bernard
name: Kamson, David O
name: Kitange, Gaspar J
name: BTCA
name: Cheserem, Beverly
name: Anunobi, Charles C
name: Osei-Tutu, Lawrence
name: Okemwa, Minda
name: Heronima, Kashaigili
name: Idowu, Olufemi E
name: Totimeh, Teddy
name: Karekezi, Claire
Publisher: name: Oxford University Press (OUP)
Year: 2024

description:
Background Brain tumors represent a significant global health challenge, with rising incidence and mortality impacting individuals worldwide and contributing to cancer-related morbidity and mortality. In Africa, this burden is exacerbated by limited access to advanced diagnostics, treatment options, and multi-disciplinary care, compounded by the absence of standardized cancer registration and tumor biobanking. The introduction of molecular diagnostics, as outlined in the 2021 WHO CNS tumor classification, adds complexity to brain tumor management, particularly in regions with scarce resources. Methods To address these issues, the Brain Tumor Consortium for Africa (BTCA) was established in 2023, bringing together experts to improve CNS tumor diagnosis, patient care, and research. The initial project, conducted via an electronic questionnaire, aimed to assess neuro-oncology capacity across SSA. Results The study revealed significant gaps, with a limited number of institutions incorporating molecular subtyping into their diagnostic algorithms. The consortium’s efforts focus on enhancing local data use, informing public policy, and promoting collaboration to advance neuro-oncology practices in Africa. By fostering a network enlisting the expertise of collaborators in the field of neurosurgery, neurology, neuropathology, anatomic pathology, and medical and radiation oncology, the BTCA seeks to improve brain tumor management through better diagnostics, infrastructure and policy advocacy. Future directions include expanding molecular diagnostic capabilities, standardizing brain tumor biobanking, enhancing data collection, and advocating for improved brain tumor care in national health agendas. Conclusion The BTCA represents a pioneering model of collaboration and innovation in addressing the unique challenges of brain tumor care in Africa.
Clinician Experiences With Integrative Group Medical Visits for Chronic Pain
Creators: name: Gardiner, Paula
name: Roth, Isabel
name: Jackson, Mary
name: Leeman, Jennifer
Publisher: name: SAGE Publications
Year: 2024

description:
Background The well-being of healthcare teams is an important consideration when seeking to improve patient experience and quality of care. Prior studies have found that changes to working conditions are most effective in improving clinician well-being. Integrative Group Medical Visits (IGMVs) modify working conditions in ways that have potential to improve clinician experience.ObjectiveThe objective of this study was to understand healthcare teams’ experiences with IGMVs. Methods In this qualitative study, interviews were conducted via Zoom and telephone with 21 clinicians, administrators, and staff from safety-net healthcare settings throughout the United States (U.S.) who have implemented IGMVs for patients with chronic pain. Interviews included questions about clinician experience, well-being, and satisfaction with the IGMV model. Interviews were recorded, transcribed, and coded using thematic content analysis by a team of trained qualitative researchers. Results The authors identified five themes describing how IGMV positively affected clinician well-being: organizational supports, human-centered engagement, collaboration with an interprofessional team, provision of guideline-concordant care, and enhanced meaning and purpose for the clinicians. Conclusion The current study was the first to use interviews from healthcare teams who have implemented IGMV to assess their experience. The themes identified warrant further investigation into IGMVs as a strategy to promote clinician well-being and mitigate aspects of burnout.
OAIS CASE STUDY:  A COMPARISON OF THE NUVOLO SOFTWARE PLATFORM USING THE “AUDIT AND CERTIFICATION OF TRUSTWORTHY DIGITAL REPOSITORIES, RECOMMENDED PRACTICE”
Creators: name: LeVine, Robert
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2024

description:
This case study provides a qualitative assessment using the standards within the “Audit and Certification of Trustworthy Digital Repositories, Recommended Practice” (CCSDS 652.0-M-1) and the Integrated Workplace Management Software (IWMS) called Nuvolo. The CCSDS 652.0-M-1 is an implementation of the Open Archival Information System (OAIS) reference model. This comparison evaluates the Nuvolo Facilities Management application within the IWMS suite. An IWMS includes a comprehensive information management function relevant to sustaining long-term databases like a repository. Can ISO 16363 metrics be used to assess non-repository database applications? The focus of this comparison is to decide where the OAIS recommendations that are listed in ISO 16363 are best applied to a cloud-hosted application like Nuvolo. The analysis also provides a study of the ServiceNow platform's viability as a compliant host for digital repositories under the current standards.
Partial long-term clinical improvement after a BCG challenge in systemic lupus erythematosus-prone mice
Creators: name: Mora, Valentina P.
name: Quero, Francisco B.
name: Troncoso-Bravo, Tays
name: Orellana, Claudia
name: Pereira, Patricia
name: Mackern-Oberti, Juan P.
name: Funes, Samanta C.
name: Soto, Jorge A.
name: Bohmwald, Karen
name: Bueno, Susan M.
name: Kalergis, Alexis M.
Publisher: name: Taylor & Francis
Year: 2024
Subjects: subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Physiology
subject: Ecology
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified

description: Systemic Lupus Erythematosus (SLE) is an autoimmune disorder that causes a breakdown of immune tolerance. Current treatments mainly involve general immunosuppression, increasing the risk of infections. On the other hand, Bacillus Calmette-Guérin (BCG) has been investigated as a potential therapy for autoimmune diseases in recent years, prompting an ongoing investigation. This study aimed to evaluate the effect of BCG vaccination on early and late clinical presentation of SLE in a murine disease model. MRL/MPJ-Faslpr mice were immunized with BCG or treated with PBS as a control. The progress of the disease was evaluated at 27 days post-immunization (dpi) (early) and 56 dpi (late). Clinical parameters and proteinuria were monitored. Blood samples were collected for measurement of antinuclear antibodies (ANAs), anti-double-stranded DNA (anti-dsDNA), and cytokine determination was performed using ELISA. Samples collected from mice were analyzed by flow cytometry and histopathology. We observed a clinical improvement in BCG-treated mice, reduced proteinuria in the latter stages of the disease, and decreased TNF-α. However, BCG did not elicit significant changes in ANAs, anti-dsDNA, histopathological scores, or immune cell infiltration. BCG was only partially beneficial in an SLE mouse model, and further research is needed to determine whether the immunity induced by this vaccine can counteract lupus’s autoimmune response.
Dysregulated CXCL12 expression in osteoblasts promotes B-lymphocytes preferentially homing to the bone marrow in MRL/lpr mice
Creators: name: Zheng, Wenjuan
name: Tang, Yu
name: Cheng, Mengwei
name: Ma, Cui
name: Fei, Xiaoming
name: Shi, Wei
Publisher: name: Taylor & Francis
Year: 2024
Subjects: subject: Biochemistry
subject: Molecular Biology
subject: Environmental Sciences not elsewhere classified
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: FOS: Biological sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: Developmental Biology
subject: Cancer
subject: Hematology
subject: Virology
subject: Computational Biology

description: Objective: Todetect the abnormal distribution of B-lymphocytes between peripheral and bone marrow (BM) compartments and explore the mechanism of abnormal chemotaxis of B-lymphocytes in lupus subjects. Methods: The proportions of CXC chemokine receptor (CXCR)4+ B cells and CFDA-labeled MRL/lpr-derived B cells were detected by flow cytometry. The levels of CXC chemokine ligand (CXCL)12in peripheral blood (PB)were measured by ELISA. The migrated B cells to osteoblasts (OBs) was measured by transwell migration assay. The relative spatial position of B cells, OBs and CXCL12 was presented by Immunofluorescence assay. Results: Firstly, we found that the percentage of CXCR4+ B cells was lower in PB and higher in the BM from both MRL/lpr mice and patientswith Systemic lupus erythematosus (SLE). Secondly, OBs from MRL/lpr mice produced more CXCL12 than that from C57BL/6 mice. Besides, MRL/lpr-derived OBs demonstrated more potent chemotactic ability toward B-lymphocytes than control OBs by vitro an vivo. Additionally, more B-lymphocytes were found to co-localize with OBs within the periosteal zone of bone in MRL/lpr mice. Lastly, the percentages of CXCR4+B cells were found to be negatively correlated with serum Immunoglobulin (Ig) G concentration, moreover, BM CXCL12 levels were found to be positively correlated with SLE disease activity index Score and negatively correlated with serum Complement3 (C3) concentration. Conclusions: our results indicated that there is a shifted distribution of B-lymphocytes between BM and peripheral compartments in both SLE patients and MRL/lpr mice. Besides, the up-regulated levels of CXCL12 in OBs was indicated to contribute to the enhanced chemotactic migration and anchorage of B-lymphocytes to OBs.
Integrated bioinformatics analysis identifies autophagy-associated genes as candidate biomarkers and reveals the immune infiltration landscape in psoriasis
Creators: name: Bai, Sixian
name: Cheng, Hongyu
name: Li, Hao
name: Bo, Peng
Publisher: name: Taylor & Francis
Year: 2024
Subjects: subject: Biochemistry
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Mathematical Sciences not elsewhere classified
subject: Computational Biology

description: Autophagy is implicated in the pathogenesis of psoriasis. We aimed to identify autophagy-related biomarkers in psoriasis via an integrated bioinformatics approach. We downloaded the gene expression profiles of GSE30999 dataset, and the “limma” package was applied to identify differentially expressed genes (DEGs). Then, differentially expressed autophagy-related genes (DEARGs) were identified via integrating autophagy-related genes with DEGs. CytoHubba plugin was used for the identification of hub genes and verified by the GSE41662 dataset. Subsequently, a series of bioinformatics analyses were employed, including protein–protein interaction network, functional enrichment, spearman correlation, receiver operating characteristic, and immune infiltration analyses. One hundred and one DEARGs were identified, and seven DEARGs were identified as hub genes and verified using the GSE41662 dataset. These validated genes had good diagnostic value in distinguishing psoriasis lesions. Immune infiltration analysis indicated that ATG5, SQSTM1, EGFR, MAPK8, MAPK3, MYC, and PIK3C3 were correlated with infiltration of immune cells. Seven DEARGs, namely ATG5, SQSTM1, EGFR, MAPK8, MAPK3, MYC, and PIK3C3, may be involved in the pathogenesis of psoriasis, which expanded the understanding of the development of psoriasis and provided important clinical significance for treatment of this disease.
Circ_0036490 and DKK1 competitively bind miR-29a to promote lipopolysaccharides-induced human gingival fibroblasts injury
Creators: name: Wu, Yeke
name: Li, Bin
name: Deng, Disi
name: Zhou, Hongling
name: Liu, Min
name: Ai, Huangping
name: Xin, Yilin
name: Hua, Weihan
name: Zhao, Lixing
name: Li, Li
Publisher: name: Taylor & Francis
Year: 2024
Subjects: subject: Biophysics
subject: Biochemistry
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Physiology
subject: Pharmacology
subject: Chemical Sciences not elsewhere classified
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Cancer

description: MicroRNA (miRNA) plays a regulatory role in periodontitis. This study aimed to explore whether miR-29a could affect lipopolysaccharides (LPSs)-induced injury in human gingival fibroblasts (HGFs) through the competitive endogenous RNAs (ceRNA) mechanism. Periodontal ligament (PDL) tissues and HGFs were derived from patients with periodontitis and healthy volunteers. Periodontitis cell model was established by treating HGFs with LPS. Expression levels of circ_0036490, miR-29a, and DKK1 were evaluated by the reverse transcription quantitative real-time PCR (RT-qPCR) method. Western blotting assay was performed to assess protein expression levels of pyroptosis-related proteins and Wnt signalling related proteins. Cell viability was evaluated by cell counting kit-8 (CCK-8) assay. Concentration of lactate dehydrogenase (LDH), interleukin (IL)-1β, and IL-18 were determined by Enzyme-linked immunosorbent assay (ELISA). Pyroptosis rate were determined by flow cytometry assay to evaluate pyroptosis. The interaction between miR-29a and circ_0036490 or DKK1 was verified by dual-luciferase reporter and RNA pull-down assays. MiR-29a expression was lower in PDL tissues of patients with periodontitis than that in healthy group; likewise, miR-29a was also downregulated in LPS-treated HGFs. Overexpression of miR-29a increased cell viability and decreased pyroptosis of HGFs induced by LPS while inhibition of miR-29a exerted the opposite role. MiR-29a binds to circ_0036490 and elevation of circ_0036490 contributed to dysfuntion of LPS-treated HGFs and reversed the protection function of elevated miR-29a. In addition, miR-29a targets DKK1. Overexpression of DKK1 abrogated the effects of overexpressed miR-29a on cell vaibility, pyroptosis, and protein levels of Wnt signalling pathway of LPS-treated HGFs. Circ_0036490 and DKK1 competitively bind miR-29a to promote LPS-induced HGF injury in vitro. Wnt pathway inactivated by LPS was activated by miR-29a. Thence, miR-29a may be a promising target for periodontitis.
Animal models of neuropsychiatric systemic lupus erythematosus: deciphering the complexity and guiding therapeutic development
Creators: name: Polis, Baruh
name: Cuda, Carla M.
name: Putterman, Chaim
Publisher: name: Taylor & Francis
Year: 2024
Subjects: subject: Space Science
subject: Medicine
subject: Molecular Biology
subject: Neuroscience
subject: Ecology
subject: FOS: Biological sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Information Systems not elsewhere classified
subject: Science Policy

description: Systemic lupus erythematosus (SLE) poses formidable challenges due to its multifaceted etiology while impacting multiple tissues and organs and displaying diverse clinical manifestations. Genetic and environmental factors contribute to SLE complexity, with relatively limited approved therapeutic options. Murine models offer insights into SLE pathogenesis but do not always replicate the nuances of human disease. This review critically evaluates spontaneous and induced animal models, emphasizing their validity and relevance to neuropsychiatric SLE (NPSLE). While these models undoubtedly contribute to understanding disease pathophysiology, discrepancies persist in mimicking some NPSLE intricacies. The lack of literature addressing this issue impedes therapeutic progress. We underscore the urgent need for refining models that truly reflect NPSLE complexities to enhance translational fidelity. We encourage a comprehensive, creative translational approach for targeted SLE interventions, balancing scientific progress with ethical considerations to eventually improve the management of NPSLE patients. A thorough grasp of these issues informs researchers in designing experiments, interpreting results, and exploring alternatives to advance NPSLE research.
Identification of macrophage-related molecular subgroups and risk signature in colorectal cancer based on a bioinformatics analysis
Creators: name: Liu, Qi
name: Liao, Li
Publisher: name: Taylor & Francis
Year: 2024
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Mathematical Sciences not elsewhere classified
subject: Cancer

description: Macrophages play a crucial role in tumor initiation and progression, while macrophage-associated gene signature in colorectal cancer (CRC) patients has not been investigated. Our study aimed to identify macrophage-related molecular subgroups and develop a macrophage-related risk model to predict CRC prognosis. The mRNA expression profile and clinical information of CRC patients were obtained from TCGA and GEO databases. CRC patients from TCGA were divided into high and low macrophage subgroups based on the median macrophage score. The ESTIMATE and CIBERSORT algorithms were used to assess immune cell infiltration between subgroups. GSVA and GSEA analyses were performed to investigate differences in enriched pathways between subgroups. Univariate and LASSO Cox regression were used to build a prognostic risk model, which was further validated in the GSE39582 dataset. A high macrophage score subgroup was associated with poor prognosis, highly activated immune-related pathways and an immune-active microenvironment. A total of 547 differentially expressed macrophage-related genes (DEMRGs) were identified, among which seven genes (including RIMKLB, UST, PCOLCE2, ZNF829, TMEM59L, CILP2, DTNA) were identified by COX regression analyses and used to build a risk score model. The risk model shows good predictive and diagnostic values for CRC patients in both TCGA and GSE39852 datasets. Furthermore, multivariate Cox regression analysis showed that the risk score was an independent risk factor for overall survival in CRC patients. Our findings provided a novel insight into macrophage heterogeneity and its immunological role in CRC. This risk score model may serve as an effective prognostic tool and contribute to personalised clinical management of CRC patients.
IL-4 polymorphisms (rs2227284, rs2243267, and rs2243270) are associated with reduced risk of rheumatoid arthritis
Creators: name: Liu, Xiaoli
name: Mai, Huqiang
name: Wang, Liang
name: Zhang, Hengxun
name: Li, Xuemei
name: Li, Xuguang
name: Wang, Li
Publisher: name: Taylor & Francis
Year: 2024
Subjects: subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Physiology
subject: Environmental Sciences not elsewhere classified
subject: Ecology
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Mathematical Sciences not elsewhere classified
subject: Cancer

description: Rheumatoid arthritis (RA) is a chronic autoimmune disease, and understanding its genetic and molecular basis is crucial for early diagnosis, treatment, and prevention. This study aims to explore the association between IL-4 polymorphisms (rs2227284, rs2243267, rs2243270, and rs2243283) and RA risk. The four IL-4 polymorphisms were genotyped in 493 RA patients and 493 healthy controls using Agena MassARRAY. Logistic regression analysis calculated odds ratio (OR) and 95% confidence interval (CI) to estimate the relationship between IL-4 polymorphisms and RA risk. Overall analysis revealed that rs2243267 (GG vs. CC: OR = 0.26, FDR-p = .032; Recessive: OR = 0.27, FDR-p = .048) and rs2243270 (AA vs. GG: OR = 0.26, FDR-p = .024; Recessive: OR = 0.27, FDR-p = .024) were associated with a decreased risk of RA. Stratified analysis indicated that rs2243267 and rs2243270 were correlated with reduced RA risk in female, smoking, BMI <24, and drinking population; rs2227284 was associated with a decreased RA risk in BMI <24 and drinking population. Moreover, rs2243267 and rs2243270 were significantly associated with reduced ACPA positivity. Our findings suggest that IL-4 polymorphisms (rs2227284, rs2243267, and rs2243270) act as protective factors for RA in the Chinese Han population.
Alteration of reactive oxygen species master transcription factor Nrf2 in keratinocytes exposed to monoclonal pathogenic antibody AK23 against desmoglein-3 in pemphigus vulgaris
Creators: name: Alabeedi, Faris Mohsin Ali
Publisher: name: Taylor & Francis
Year: 2024
Subjects: subject: Biochemistry
subject: Space Science
subject: Medicine
subject: Cell Biology
subject: Molecular Biology
subject: Physiology
subject: FOS: Biological sciences
subject: Chemical Sciences not elsewhere classified
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Computational Biology

description: Keratinocytes in mucosal and skin tissues maintain tissue integrity via desmosomes and desmoglein-3 (Dsg3). Pemphigus Vulgaris (PV) is a life-threatening autoimmune blistering disease characterized by autoantibodies against Dsg3, disrupting desmosomes. Nuclear factor erythroid 2–related factor 2 (Nrf2) regulates oxidative stress responses crucial for skin tissue protection. Although the pathogenesis of PV is known, the detailed molecular events remain unclear. This study investigates changes in Nrf2 expression in keratinocytes following pathogenic anti-Dsg3 antibody AK23 exposure, using dose- and time-dependent studies employing immunofluorescence analysis. N/TERT keratinocytes were cultured in keratinocytes serum-free medium and treated with AK23 at varying doses (5 µg/mL,40µg/mL,75µg/mL) and durations (2, 6, 24 h). Immunofluorescence staining was performed to assess the expression of Nrf2 and Dsg3. All fluorescent images were analyzed using ImageJ software. A dose-dependent increase in Dsg3 was noted following AK23 treatment, while Nrf2 expression and subcellular localization varied. Time-course analyses showed decreased Nrf2 at 24 h and increased Dsg3 levels. Early time-point (2 and 6 h) variations were evident in Nrf2 levels. This study highlights the impact of AK23 on Nrf2 expression, potentially disrupting Nrf2-mediated cytoprotection and implicating oxidative stress (ROS generation) in PV pathogenesis. Further investigation is necessary to validate the findings.
CircNRIP1 promotes proliferation, migration and phenotypic switch of Ang II-induced HA-VSMCs by increasing CXCL5 mRNA stability via recruiting IGF2BP1
Creators: name: Cao, Xianzhao
name: Fang, Hongyan
name: Zhou, Longshu
Publisher: name: Taylor & Francis
Year: 2024
Subjects: subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Physiology
subject: Chemical Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Cancer

description: Circular RNA (circRNA) has been found to be differentially expressed and involved in regulating the processes of human diseases, including thoracic aortic dissection (TAD). However, the role and mechanism of circNRIP1 in the TAD process are still unclear. GEO database was used to screen the differentially expressed circRNA and mRNA in type A TAD patients and age-matched normal donors. Angiotensin II (Ang II)-induced human aortic vascular smooth muscle cells (HA-VSMCs) were used to construct TAD cell models. The expression levels of circNRIP1, NRIP1, CXC-motif chemokine 5 (CXCL5) and IGF2BP1 were detected by quantitative real-time PCR. Cell proliferation and migration were determined by EdU assay, transwell assay and wound healing assay. The protein levels of synthetic phenotype markers, contractile phenotype markers, CXCL5 and IGF2BP1 were tested by western blot analysis. The interaction between IGF2BP1 and circNRIP1/CXCL5 was confirmed by RIP assay, and CXCL5 mRNA stability was assessed by actinomycin D assay. CircNRIP1 was upregulated in TAD patients and Ang II-induced HA-VSMCs. Knockdown of circNRIP1 suppressed Ang II-induced proliferation, migration and phenotypic switch of HA-VSMCs. Also, high expression of CXCL5 was observed in TAD patients, and its knockdown could inhibit Ang II-induced HA-VSMCs proliferation, migration and phenotypic switch. Moreover, CXCL5 overexpression reversed the regulation of circNRIP1 knockdown on Ang II-induced HA-VSMCs functions. Mechanistically, circNRIP1 could competitively bind to IGF2BP1 and subsequently enhance CXCL5 mRNA stability. CircNRIP1 might contribute to TAD progression by promoting CXCL5 mRNA stability via recruiting IGF2BP1.
A whole blood-based functional assay to characterize immunoglobulin A effector functions
Creators: name: Bacon, Alice
name: García, Celia Cartagena
name: van Schie, Karin A.
name: Toes, René E. M.
name: Busnel, Jean-Marc
Publisher: name: Taylor & Francis
Year: 2024
Subjects: subject: Biochemistry
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Physiology
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Plasma Physics

description: Most investigations on the immune cell-activating potency of IgA used purified total IgA and/or specific isolated cell populations. As IgA2 has been reported to be more pro-inflammatory than IgA1, we aimed to employ a fast and convenient whole blood-based assay to individually probe the capacity of the two IgA subclasses to activate immune cells in close physiological conditions. To this end, whole blood from healthy donors (n = 10) was stimulated with immobilized IgA1, IgA2m1 or IgA2m2 (the two main allotypic variants of IgA2). Activation of major leukocyte subsets was measured using a 10-color flow cytometry panel providing access to the expression of 5 activation markers on 6 different immune cell subsets. While capturing some heterogeneity of responses among donors, IgA2m1 and IgA2m2 systematically showed a stronger activation profile compared to IgA1 in a variety of dimensions. For example, both IgA2 allotypes led to stronger modulations of CD54, CD11b, CD62L, CD66b or CD69, on both or either monocytes or neutrophils, indicating a more pronounced pro-inflammatory effect for this subclass than IgA1. By taking into account donor-specific soluble and cellular components this whole blood-based functional approach provides new perspectives to further investigate IgA effector functions in mechanistic studies and/or translational research.
Scaria Bolivar 1887
Creators: name: Itrac-Bruneau, Raphaëlle
name: Doucet, Guillaume
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Orthoptera
Tetrigidae
Scaria

description: Genre Scaria Bolívar, 1887
REMARQUE
Le genre Scaria Bolívar, 1887 est très proche des genres Rehnidium Grant, 1956 et Batrachidea Serville, 1838. Les principaux critères retenus par Grant (1956, 1962), Cadena-Castañeda et al. (2019) et Kasalo et al. (2021) pour le distinguer sont: 1) la présence d’une forte épine crochue en partie antérieure du pronotum; 2) un pronotum atteignant ou dépassant l’extrémité des fémurs postérieurs; 3) des ailes toujours bien développées; 4) un pronotum plat; 5) les fémurs médians terminés par une forte épine; 6) la forme de la plaque sous-génitale des femelles; 7) une grande taille; 8) la côte frontale légèrement saillante; et 9) des tegmina noirâtres. Cependant certains critères ne sont pas constants tels que la couleur des tegmina (parfois bruns, voir Grant 1962) et la forme des plaques sous-génitales des femelles (très variable au sein des espèces du genre, voir Cadena-Castañeda et al. (2019)), et ne peuvent donc pas être utilisés. D’après nos observations, il est préférable d’utiliser la combinaison des cinq premiers critères mentionnés ci-dessus.
description: Published as part of Itrac-Bruneau, Raphaëlle & Doucet, Guillaume, 2023, Apport des collections du Muséum national d'Histoire naturelle et de Didier Morin à la connaissance des Batrachideinae et Lophotettiginae (Orthoptera, Tetrigidae) de Guyane, pp. 601-634 in Zoosystema 45 (20) on page 610, DOI: 10.5252/zoosystema2023v45a20, http://zenodo.org/record/10064321
Lophotettix alticristatus * Hancock 1909
Creators: name: Itrac-Bruneau, Raphaëlle
name: Doucet, Guillaume
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Orthoptera
Tetrigidae
Lophotettix
Lophotettix alticristatus

description: Lophotettix alticristatus* Hancock, 1909
(Figs 12A; 15A)
Lophotettix alticristatus Hancock, 1909. — Silva et al. 2019b: 348. — Kasalo et al. 2022: 229.
LOCALITÉ TYPE. — Brésil.
DISTRIBUTION. — Brésil, Pérou, Équateur (Kasalo et al. 2022) et Guyane (nouveau pour le département).
MATÉRIEL EXAMINÉ. — Guyane • 1 ind; [Régina], Nouragues, Saut Pararé; 04°02’26.8”N, 52°40’35.2”W; piège cryldé; 20.VII.2009; SEAG leg.; coll. DM.
description: Published as part of Itrac-Bruneau, Raphaëlle & Doucet, Guillaume, 2023, Apport des collections du Muséum national d'Histoire naturelle et de Didier Morin à la connaissance des Batrachideinae et Lophotettiginae (Orthoptera, Tetrigidae) de Guyane, pp. 601-634 in Zoosystema 45 (20) on page 624, DOI: 10.5252/zoosystema2023v45a20, http://zenodo.org/record/10064321
Arnobia Stal 1876
Creators: name: Liu, Chun-Xiang
Publisher: name: Zenodo
Year: 2011
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Orthoptera
Phaneropteridae
Arnobia

description: Arnobia Stål, 1876
Type species: Locusta (Phaneroptera) pilipes Haan, 1842 (Japan).
Arnobia Stål, 1876, Bihang Svenska Akad. iv (5): 56; Stål, 1878, Bih. Svenska Vet. Akad. Hnadl. Iv (5): 56; Brunner von Wattenwyl, 1878, Verh. zool.-bot. Ges. Vien 28: 19, 162; Brunner von Wattenwyl, 1891, Verh. zool.-bot. Ges. Vien. 41: 11; Jacobson & Bianchi, 1902-3, Orth. and Pseud. Russ. Emp.: 324, 336, 374; Kirby, 1906, Syn. Cat. Orth. 2: 423; Karny, 1923, Jour. Roy. Asiat. Soc., Malay. Branch 1: 139; Karny, J. Fed. Malay States Mus. 13(2-3): 88; Bey-Bieko, 1954, Fauna of the USSR, Vol.2, No. 2: 115.
Six species and subspecies are recorded mostly distributed in Thailand, Vietnam, Japan and Indonesia. Here we report two new species from Guangxi Province and Hainan Island, China.
Species included: type species, Locusta (Phaneroptera) trichopus Haan, 1842 (Java), Stictophaula ocellata Ingrisch, 1994 (Thailand), A. vietensis Gorochov, 1998 (Vietnam), A. inocellata Gorochov, 1998 (Thailand), A. pilipes tropica Gorochov, 1998.
Description. Bei-Bienko (1954).
description: Published as part of Liu, Chun-Xiang, 2011, Brief Summary of Holochlorini (Orthoptera: Tettigoniidae: Phaneropterinae), with Description of Seven New Species from China, pp. 35-52 in Zootaxa 2931 on page 36, DOI: 10.5281/zenodo.202272