title: ÐÐ°Ð²Ð¸ÑÐ¸Ð¼Ð¾ÑÑÑ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ñ Ð¾Ð½Ð´ÑÐ¾Ð³ÐµÐ½Ð½Ð¾Ð¹ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐ¸ Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÑ ÑÑÑÐ¾Ð¼Ð°Ð»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð¾Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ MAP- Ð¸ PI3-ÐºÐ¸Ð½Ð°Ð·Ð½ÑÑ ÑÐ¸Ð³Ð½Ð°Ð»ÑÐ½ÑÑ ÐºÐ°ÑÐºÐ°Ð´Ð¾Ð²
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: The dependence of the efficiency of chondrogenic differentiation of human mesenchymal stromal cells on the activity of MAP - and PI3-kinase signaling cascades
Creators: name: ÐÑÐ¾Ð²ÐºÐ¸Ð½Ð°, ÐÐ½Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸ (Ð¡Ð)
Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÐµ ÑÑÑÐ¾Ð¼Ð°Ð»ÑÐ½ÑÐµ Ð¡Ð ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ñ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° (ÑÐÐ¡Ð)
Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐ°
Ñ Ð¾Ð½Ð´ÑÐ¾Ð³ÐµÐ½Ð½Ð°Ñ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐ°
Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²Ð¾ÑÐ½ÑÐ¹ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð» Ð¡Ð
MAP-ÐºÐ¸Ð½Ð°Ð·Ð½ÑÐ¹ ÑÐ¸Ð³Ð½Ð°Ð»ÑÐ½ÑÐ¹ Ð¿ÑÑÑ
PI3-ÐºÐ¸Ð½Ð°Ð·Ð½ÑÐ¹ ÑÐ¸Ð³Ð½Ð°Ð»ÑÐ½ÑÐ¹ Ð¿ÑÑÑ
stem cells (SC)
Human Endometrial Mesenchymal stromal SC (eMSC)
Differentiation
Chondrogenic differentiation
SC differentiation potential
MAP-kinase signaling pathway
PI3-kinase signaling pathway

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð° Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð¹ ÑÐ¾Ð½Ð´ÑÐ¾Ð³ÐµÐ½Ð½Ð¾Ð¹ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐ¸ Ð¼ÐµÐ·ÐµÐ½ÑÐ¸Ð¼Ð½ÑÑ ÑÑÑÐ¾Ð¼Ð°Ð»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð¸Ð· Ð´ÐµÑÐºÐ²Ð°Ð¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ñ Ð² Ð¼ÐµÐ½ÑÑÑÑÐ°Ð»ÑÐ½Ð¾Ð¹ ÐºÑÐ¾Ð²Ð¸ Ð·Ð´Ð¾ÑÐ¾Ð²ÑÑ Ð´Ð¾Ð½Ð¾ÑÐ¾Ð² (ÑÐÐ¡Ð). Ð¦ÐµÐ»Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â Ð¿ÑÐ¾Ð´ÐµÐ¼Ð¾Ð½ÑÑÑÐ¸ÑÐ¾Ð²Ð°ÑÑ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾ÑÑÑ ÑÐ¾Ð½Ð´ÑÐ¾Ð³ÐµÐ½Ð½Ð¾Ð¹ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐ¸ Ð² 2D-ÐºÑÐ»ÑÑÑÑÐµ ÑÐÐ¡Ð Ð¸ Ð¸Ð·ÑÑÐ¸ÑÑ Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾ÑÑÑ ÐµÐµ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð¾Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ MAP- Ð¸ PI3-ÐºÐ¸Ð½Ð°Ð·Ð½ÑÑ ÑÐ¸Ð³Ð½Ð°Ð»ÑÐ½ÑÑ ÐºÐ°ÑÐºÐ°Ð´Ð¾Ð². Ð¡ÑÐµÐ´Ð¸ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð¼ÐµÑÐ¾Ð´Ð¾Ð², Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð½ÑÑ Ð² ÑÐ°Ð±Ð¾ÑÐµ: ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÐºÐ»ÐµÑÐ¾Ðº, ÑÐºÑÑÑÐ°ÐºÑÐ¸Ñ Ð¼Ð ÐÐ Ð¸Ð· ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð»Ð¸Ð·Ð°ÑÐ¾Ð², ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÐ·Ñ (Ð±ÐµÐ»ÐºÐ¾Ð²ÑÐ¹, Ð ÐÐ Ð¸ ÐÐÐ), Ð¸Ð¼Ð¼ÑÐ½Ð¾Ð±Ð»Ð¾ÑÐ¸Ð½Ð³, ÐÐ¢-ÐÐ¦Ð  Ð² ÑÐµÐ¶Ð¸Ð¼Ðµ ÑÐµÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð²ÑÐµÐ¼ÐµÐ½Ð¸, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¾ÐºÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ðµ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð² Ð°Ð»ÑÐ¸Ð°Ð½Ð¾Ð²ÑÐ¼ ÑÐ¸Ð½Ð¸Ð¼ Ð¸ ÑÐ°ÑÑÐ°Ð½Ð¸Ð½Ð¾Ð¼ Ð. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð° Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð° Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾ÑÑÑ ÑÐ¾Ð½Ð´ÑÐ¾Ð³ÐµÐ½Ð½Ð¾Ð¹ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐ¸ ÑÐÐ¡Ð Ð² Ð¼Ð¾Ð½Ð¾ÑÐ»Ð¾Ðµ (2D-ÐºÑÐ»ÑÑÑÑÐµ) Ð¸ Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐ° ÑÑÐ¾Ð³Ð¾ Ð¿ÑÐ¾ÑÐµÑÑÐ° Ð² ÑÐµÑÐµÐ½Ð¸Ðµ 14-ÑÐ¸ Ð´Ð½ÐµÐ¹; ÑÐ°ÐºÐ¶Ðµ Ð±ÑÐ»Ð° Ð²ÑÑÐ²Ð»ÐµÐ½Ð° Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾ÑÑÑ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐ¸ Ð¾Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ MAP- Ð¸ PI3-ÐºÐ¸Ð½Ð°Ð·Ð½ÑÑ ÑÐ¸Ð³Ð½Ð°Ð»ÑÐ½ÑÑ ÐºÐ°ÑÐºÐ°Ð´Ð¾Ð², ÑÑÐ¾ Ð´Ð°ÐµÑ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÐµÐ½Ð¸Ðµ Ð¾ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾Ð¼ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ðµ Ð¿ÑÐ¾ÑÐµÑÑÐ°. ÐÑÑÐµÐ¾Ð¿Ð¸ÑÐ°Ð½Ð½ÑÐµ ÑÐµÐ½Ð¾Ð¼ÐµÐ½Ñ ÑÐ¾ÑÑÐ°Ð²Ð»ÑÑÑ Ð½Ð°ÑÑÐ½ÑÑ Ð½Ð¾Ð²Ð¸Ð·Ð½Ñ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ.
description: This work is devoted to the study of the mechanism of induced chondrogenic differentiation of human mesenchymal stromal cells obtained from desquamated endometrium in the menstrual blood of healthy donors (eMSCs). The aim of the study was to demonstrate the possibility of chondrogenic differentiation in 2D culture of eMSCs and to study the dependence of its effec-tiveness on the activity of MAP-and PI3-kinase signaling cascades. The experimental methods used in this work include cell culture, mRNA extraction from cell lysates, electrophoresis (protein, RNA, and DNA), immunoblotting, real-time RT-PCR, and staining of cell preparations with alcyan blue and safranin O. As a result of this work, the possibility of chondrogenic differentiation of eMSCs in a monolayer (2D culture) and the dynamics of this process over 14 days were shown; the dependence of the differentiation efficiency on the activity of MAP - and PI3-kinase signaling cascades was also revealed, which gives an idea of the molecular mechanism of the process. The above-described phenomena constitute the scientific novelty of the presented research.