title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ Ð°Ð½ÑÐ¸Ð¾ÐºÑÐ¸Ð´Ð°Ð½ÑÐ¾Ð² Ð½Ð° Ð¿ÑÐ¾Ð»Ð¸ÑÐµÑÐ°ÑÐ¸Ñ Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ñ
title: Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÑÐºÐ°Ñ ÑÐ°Ð±Ð¾ÑÐ°
titleType: Subtitle
Creators: name: ÐÐ¾ÑÐ½Ð¸ÐµÐ½ÐºÐ¾, Ð®Ð»Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2016
Subjects: Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÐµ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸
Ð°Ð½ÑÐ¸Ð¾ÐºÑÐ¸Ð´Ð°Ð½ÑÑ
ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐ¹ ÑÐ¸ÐºÐ»
Ð¿Ð¾Ð²ÑÐµÐ¶Ð´ÐµÐ½Ð¸Ðµ ÐÐÐ
ÐºÐ»ÐµÑÐ¾ÑÐ½Ð°Ñ Ð¿ÑÐ¾Ð»Ð¸ÑÐµÑÐ°ÑÐ¸Ñ
antioxidants
mesenchymal stem cells
cell cycle
DNA damage
cells proliferation

description: Ð¦ÐµÐ»ÑÑ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¾ Ð¸Ð·ÑÑÐ¸ÑÑ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ ÑÑÐ±-ÑÐ¸ÑÐ¾ÑÐ¾ÐºÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð´Ð¾Ð· Ð°Ð½ÑÐ¸Ð¾ÐºÑÐ¸Ð´Ð°Ð½ÑÐ¾Ð² Ð½Ð° Ð¿ÑÐ¾ÑÐµÐºÐ°Ð½Ð¸Ðµ Ð¿ÑÐ¾Ð»Ð¸ÑÐµÑÐ°ÑÐ¸Ð²Ð½ÑÑ ÑÐ°Ð· ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ ÑÐ¸ÐºÐ»Ð° Ð² ÑÐ¸Ð½ÑÑÐ¾Ð½Ð¸Ð·Ð¾Ð²Ð°Ð½Ð½ÑÑ Ð¿Ð¾ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¼Ñ ÑÐ¸ÐºÐ»Ñ ÐºÑÐ»ÑÑÑÑÐ°Ñ Ð¼ÐµÐ·ÐµÐ½ÑÐ¸Ð¼Ð½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ñ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°. Ð£ÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¾, ÑÑÐ¾ Ð´Ð¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸Ðµ ÑÑÐ±ÑÐ¸ÑÐ¾ÑÐ¾ÐºÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð´Ð¾Ð· Ð°Ð½ÑÐ¸Ð¾ÐºÑÐ¸Ð´Ð°Ð½ÑÐ¾Ð² Ð² ÑÐ¾ÑÑÐ¾Ð²ÑÑ ÑÑÐµÐ´Ñ ÐÐ¡ÐÑ Ð½Ð° ÑÑÐ°Ð´Ð¸Ð¸ ÑÐ°Ð½Ð½ÐµÐ¹ ÑÐ°Ð·Ñ ÑÐ¸Ð½ÑÐµÐ·Ð° ÐÐÐ Ð¿ÑÐ¸Ð²Ð¾Ð´Ð¸Ñ Ðº Ð´Ð¾Ð·Ð¾-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾Ð¼Ñ Ð·Ð°Ð¼ÐµÐ´Ð»ÐµÐ½Ð¸Ñ ÑÐ¸Ð½ÑÐµÐ·Ð° ÐÐÐ Ð¸ Ð±Ð»Ð¾ÐºÑ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ ÑÐ¸ÐºÐ»Ð° Ð² G2/M-ÑÐ°Ð·Ðµ. ÐÐ½Ð°Ð»Ð¸Ð· Ð½Ð°ÐºÐ¾Ð¿Ð»ÐµÐ½Ð¸Ñ Ð¼Ð°ÑÐºÐµÑÐ¾Ð² ÑÐ°Ð·ÑÑÐ²Ð¾Ð² ÐÐÐ - ÑÐ¾ÑÑÐ¾ÑÐ¸Ð»Ð¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð¹ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Ð³Ð¸ÑÑÐ¾Ð½Ð° H2AX Ð¸ Ð°ÐºÑÐ¸Ð²Ð½Ð¾Ð¹ ÐÐ¢Ð-ÐºÐ¸Ð½Ð°Ð·Ñ (Ð°taxia telangiectasia mutated kinase) - Ð¿Ð¾ÐºÐ°Ð·Ð°Ð», ÑÑÐ¾ Ð±Ð»Ð¾Ðº ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ ÑÐ¸ÐºÐ»Ð° Ð² G2/M-ÑÐ°Ð·Ðµ Ð¿ÑÐ¸ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÐµ ÐÐ¡ÐÑ Ð°Ð½ÑÐ¸Ð¾ÐºÑÐ¸Ð´Ð°Ð½ÑÐ°Ð¼Ð¸ Ð¾Ð±ÑÑÐ»Ð¾Ð²Ð»ÐµÐ½ ÐÐ¢Ð-Ð¾Ð¿Ð¾ÑÑÐµÐ´Ð¾Ð²Ð°Ð½Ð½ÑÐ¼ Ð¾ÑÐ²ÐµÑÐ¾Ð¼ Ð½Ð° Ð¿Ð¾Ð²ÑÐµÐ¶Ð´ÐµÐ½Ð¸Ñ ÐÐÐ. ÐÑÐ¾Ð¼Ðµ ÑÐ¾Ð³Ð¾, ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¾, ÑÑÐ¾ Ð·Ð°Ð¼ÐµÐ´Ð»ÐµÐ½Ð¸Ðµ Ð¿ÑÐ¾ÑÐµÐºÐ°Ð½Ð¸Ñ ÑÐ°Ð·Ñ ÑÐ¸Ð½ÑÐµÐ·Ð° ÐÐÐ Ð¿Ð¾Ð´ Ð´ÐµÐ¹ÑÑÐ²Ð¸ÐµÐ¼ Ð°Ð½ÑÐ¸Ð¾ÐºÑÐ¸Ð´Ð°Ð½ÑÐ¾Ð² ÑÐ¾Ð¿ÑÐ¾Ð²Ð¾Ð¶Ð´Ð°ÐµÑÑÑ Ð¿Ð¾Ð½Ð¸Ð¶ÐµÐ½Ð¸ÐµÐ¼ ÑÑÐ¾Ð²Ð½Ñ ÑÐ¸ÐºÐ»Ð¸Ð½Ð° Ð - Ð¾Ð´Ð½Ð¾Ð³Ð¾ Ð¸Ð· ÐºÐ»ÑÑÐµÐ²ÑÑ Ð±ÐµÐ»ÐºÐ¾Ð², ÑÐµÐ³ÑÐ»Ð¸ÑÑÑÑÐ¸Ñ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐ¹ ÑÐ¸ÐºÐ» Ð½Ð° ÑÑÐ°Ð´Ð¸Ð¸ ÑÐ¸Ð½ÑÐµÐ·Ð° ÐÐÐ.
description: The aim of this work was to examine the impact of sub-cytotoxic doses of antioxidants on the dynamics of the proliferative phases of the cell cycle in the synchronized cultures of human endometrial mesenchymal stem cells (eMSCs). It was shown that the addition of sub-cytotoxic doses of antioxidants to the growth medium of eMSCs progressing through the early S-phase of the cell cycle results in the dose-depended retardation of the DNA synthesis followed by the cell cycle arrest in G2/M-phase. Analysis of the accumulation of DNA strand break markers - phosphorylated histone H2AX and active ATM-kinase (ataxia telangiectasia mutated kinase) have shown that the cell cycle arrest is due to the ATM-mediated response to the DNA damage. Furthermore, it was found that retardation of the DNA synthesis, caused by the antioxidant treatment, was accompanied with the decrease of the intracellular level of the cyclin A protein - key cell cycle regulator of the S-phase progression.