Additional file 1 of Hidden paths to endless forms most wonderful: Complexity of bacterial motility shapes diversification of latent phenotypes
Creators: name: Rendueles, Olaya
name: Velicer, Gregory J.
Publisher: name: figshare
Year: 2020
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: FOS: Biological sciences

description: Additional file 1: Figure S1. Alternative-surface fitness patterns across treatments: per-population means. The leading edges of swarming colonies initially mixed as 1:1 evolved:marked-ancestor were sampled for the presence/absence of the marked ancestor (five samples per colony). The mean ancestor-presence proportions for each evolved population across three replicate assays are shown (data points). Gray bars correspond to the within-treatment average of the per-population means. Data for the selective-surface assays was originally published in [47]. * p 0.05 for difference from the ancestor are in black. Evolved populations are ordered left to right within each treatment set by increasing MyxoEE-3 population number. Swarming rates can be found in Additional file 1, Table S4. c. Average evolutionary change in swarming rates for each of the six treatments, expressed as a percentage increase relative to ancestor strains on their selective (as originally published in [47]) and alternative surfaces. Values shown are cross-population means of cross-replicate per-population means (N = 7, 8 or 12). *p 0.05). Horizontal lines indicate the average across clones within each category. One clone found to be a mutator (P29) [47] was excluded. Table S1. MyxoEE-3 treatments examined in this study. Minimum-generation estimates and other related information are presented in Table S1 of [47]. Table S2. Fitness of evolved populations on their alterative surface. Overall percentage of samples in which the kanamycin-marked ancestor was found at the edge of colonies that were initially composed as a 1:1 mix of the ancestor and an evolved population. Numbers in parentheses represent the number of population samples (out of five) collected from the swarm perimeter in which the ancestor was detected for each of the three replicate assays. Background shading reflects a heat map in which darkness correlates with the proportion of samples containing the ancestor. ‘HA’ and ‘SA’ indicate hard and soft agar, respectively. The corresponding data for competitions performed on each population’s MyxoEE-3 selective surface was reported in Table S2 of [47]. (The data for the control competitions between marked and unmarked ancestors on soft agar was omitted by oversight in [47] but is included here.) Table S3. Absolute swarming rates of each population on their selective and alternative surfaces. Data is expressed as mm/day. Green- and red-shaded cells correspond to significant increases and decreases in swarming rate compared to ancestor (paired t-student tests), respectively. ‘+’ and ‘–’ symbols for evolved populations indicate the direction of change relative to the proximate ancestor, irrespective of significance. Blue cells indicate populations with same-direction evolutionary-change estimates across surface types and tan cells indicate opposite-direction change estimates, irrespective of significance. Table S4. P values for differences in swarming on the alternative surface relative to the ancestor. Wilcoxon sign rank test for significant difference from 1. Table S5. Tests for correlations between evolutionary change in swarming rates on selective vs. alternative surfaces.