Additional file 1 of Graphene oxide nanofilm and chicken embryo extract decrease the invasiveness of HepG2 liver cancer cells
Creators: name: Malwina Sosnowska
name: Kutwin, Marta
name: Strojny, Barbara
name: Koczoń, Piotr
name: Jarosław Szczepaniak
name: Jaśmina Bałaban
name: Daniluk, Karolina
name: Sławomir Jaworski
name: Chwalibog, André
name: Wiesław Bielawski
name: Sawosz, Ewa
Publisher: name: figshare
Year: 2021
Subjects: subject: Biophysics
subject: Biochemistry
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Physiology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: FOS: Chemical sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Cancer
subject: Hematology

description: Additional file 1: Figure S1. Fourier transform infrared spectrum of chicken embryo liver extract (top) and graphene oxide nanofilm with addition of chicken embryo lever extract (bottom). Figure S2. Cell migration of HS-5 cells on modified surface after removing the insert (0 h) and after 24, 48, 72 h of incubation (scratch wound healing assay). Figure S3. Cell migration of HepG2 cells on modified surface after removing the insert (0 h) and after 24, 48, 72 h of incubation (scratch wound healing assay). Figure S4. Cell migration of C3A cells on modified surface after removing the insert (0 h) and after 24, 48, 72 h of incubation (scratch wound healing assay). Table S1. Wound closure rate of HS-5, HepG2 and C3A cells on modified surface. Results are expressed as percentage intercellular spaces based on ImageJ analysis. Table S2. Changes in HS-5, HepG2 and C3A cell viability using the MTT assay. Table S3. Changes in HS-5, HepG2 and C3A cell proliferation using the BrdU assay. Table S4. Changes in the expression of proliferation (pcna, ki67, mcm2) and adhesion markers (integrins, cadherins, focal adhesion kinase and catenine) in HS-5 cells by real-time PCR (one way ANOVA). The results are presented as FC values, and untreated cells are depicted as 1. Values above/below 1 indicate upregulation/downregulation of gene expression. Abbreviations: FC, fold change. Table S5. Changes in the expression of proliferation (pcna, ki67, mcm2) and adhesion markers (integrins, cadherins, focal adhesion kinase and catenine) in HepG2 cells by real-time PCR (one way ANOVA). The results are presented as FC values, and untreated cells are depicted as 1. Values above/below 1 indicate upregulation/downregulation of gene expression. Abbreviations: FC, fold change. Table S6. Changes in the expression of proliferation (pcna, ki67, mcm2) and adhesion markers (integrins, cadherins, focal adhesion kinase and catenine) in C3A cells by real-time PCR (one way ANOVA). The results are presented as FC values, and untreated cells are depicted as 1. Values above/below 1 indicate upregulation/downregulation of gene expression. Abbreviations: FC, fold change. Table S7. Cell cycle changes of HS-5, HepG2 and C3A cells using the flow cytometry. Table S8. Changes in the expression of proliferation (pcna, ki67, mcm2) and adhesion markers (integrins, cadherins, focal adhesion kinase and catenine) in HepG2 and C3A cells using two-way analysis of variance. The results are presented as FC values, and untreated cells are depicted as 1. Values above/below 1 indicate upregulation/downregulation of gene expression. Abbreviations: FC, fold change