title: ÐÐ·ÑÑÐµÐ½Ð¸Ðµ ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐ½Ð¾ÑÑÐ¸ Ð°Ð½ÑÐ¸ÑÐµÐ» ÑÑÐ²Ð¾ÑÐ¾ÑÐºÐ¸ ÐºÑÐ¾Ð²Ð¸ Ðº Ð³Ð¸Ð¿Ð¾Ñ Ð»Ð¾ÑÐ¸Ñ-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¼ Ð»Ð¸Ð¿Ð¾Ð¿ÑÐ¾ÑÐµÐ¸Ð½Ð°Ð¼ Ð½Ð¸Ð·ÐºÐ¾Ð¹ Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÐ¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Studying of the specificity of blood serum antibodies to hypochlorite-modified low-density lipoproteins
Creators: name: ÐÑÐ¸Ð³Ð¾ÑÑÐµÐ²Ð°, ÐÑÐµÐ½Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð°Ð½ÑÐ¸ÑÐµÐ»Ð°
Ñ Ð¸Ð¼Ð¸ÑÐµÑÐºÐ°Ñ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ñ
Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐµ Ð»Ð¸Ð¿Ð¾Ð¿ÑÐ¾ÑÐµÐ¸Ð½Ñ Ð½Ð¸Ð·ÐºÐ¾Ð¹ Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÐ¸
Ð¸Ð¼Ð¼ÑÐ½Ð¾ÑÐµÑÐ¼ÐµÐ½ÑÐ½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð·
antibodies
chemical modification
modified low density lipoproteins
elisa immuno assay

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¸ Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸Ðº Ð°Ð½ÑÐ¸ÑÐµÐ» ÑÑÐ²Ð¾ÑÐ¾ÑÐºÐ¸ ÐºÑÐ¾Ð²Ð¸ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ðº Ð³Ð¸Ð¿Ð¾ÑÐ»Ð¾ÑÐ¸Ñ-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¼ Ð»Ð¸Ð¿Ð¾Ð¿ÑÐ¾ÑÐµÐ¸Ð½Ð°Ð¼ Ð½Ð¸Ð·ÐºÐ¾Ð¹ Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÐ¸. ÐÐ°Ð´Ð°ÑÐ¸, ÑÐµÑÐ°ÐµÐ¼ÑÐµ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: 1. ÐÑÐ¾Ð°Ð½Ð°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°ÑÑ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½ÑÐµ Ð¸ÑÑÐ¾ÑÐ½Ð¸ÐºÐ¸, Ð² ÐºÐ¾ÑÐ¾ÑÑÑ ÑÐ°ÑÑÐ¼Ð°ÑÑÐ¸Ð²Ð°ÐµÑÑÑ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼ ÑÐ¸Ð¼Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ ÐÐÐÐ in vivo Ð¸ ÑÐ¾Ð»Ñ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ ÐÐÐÐ Ð² Ð°ÑÐµÑÐ¾Ð³ÐµÐ½ÐµÐ·Ðµ; 2. ÐÐ±Ð½Ð°ÑÑÐ¶Ð¸ÑÑ Ð°Ð½ÑÐ¸ÑÐµÐ»Ð° Ðº Ð³Ð¸Ð¿Ð¾ÑÐ»Ð¾ÑÐ¸Ñ-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¼ ÐÐÐÐ Ð² ÑÐµÐ»Ð¾Ð²ÐµÑÐµÑÐºÐ¸Ñ ÑÑÐ²Ð¾ÑÐ¾ÑÐºÐ°Ñ; 3. Ð£ÑÐ¾Ð²ÐµÑÑÐµÐ½ÑÑÐ²Ð¾Ð²Ð°ÑÑ Ð¼ÐµÑÐ¾Ð´Ð¸ÐºÑ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ ÐÐ¢ Ðº HOCl-ÐÐÐÐ Ð¸Ð· ÑÑÐ²Ð¾ÑÐ¾ÑÐºÐ¸ ÐºÑÐ¾Ð²Ð¸ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°; 4. ÐÐ¾ÐºÐ°Ð·Ð°ÑÑ ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐ½Ð¾ÑÑÑ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½ÑÑ ÐÐ¢ Ðº HOCl-ÐÐÐÐ; 5. ÐÐ¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ ÐºÐ¾Ð½ÑÑÐ°Ð½ÑÑ Ð´Ð¸ÑÑÐ¾ÑÐ¸Ð°ÑÐ¸Ð¸ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ° Ð°Ð½ÑÐ¸Ð³ÐµÐ½-Ð°Ð½ÑÐ¸ÑÐµÐ»Ð¾ Ð´Ð»Ñ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½ÑÑ ÐÐ¢ Ðº HOCl-ÐÐÐÐ. ÐÐ±ÑÐµÐºÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: ÑÑÐ²Ð¾ÑÐ¾ÑÐºÐ° ÐºÑÐ¾Ð²Ð¸ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°, Ð»Ð¸Ð¿Ð¾Ð¿ÑÐ¾ÑÐµÐ¸Ð½Ñ Ð½Ð¸Ð·ÐºÐ¾Ð¹ Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÐ¸, ÑÐµÐ»Ð¾Ð²ÐµÑÐµÑÐºÐ¸Ð¹ ÑÑÐ²Ð¾ÑÐ¾ÑÐ¾ÑÐ½ÑÐ¹ Ð°Ð»ÑÐ±ÑÐ¼Ð¸Ð½. Ð ÑÐ¾Ð´Ðµ ÑÐ°Ð±Ð¾ÑÑ Ð² ÑÑÐ²Ð¾ÑÐ¾ÑÐºÐ°Ñ ÐºÑÐ¾Ð²Ð¸ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ñ Ð°Ð½ÑÐ¸ÑÐµÐ»Ð° Ðº Ð³Ð¸Ð¿Ð¾ÑÐ»Ð¾ÑÐ¸Ñ-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¼ Ð»Ð¸Ð¿Ð¾Ð¿ÑÐ¾ÑÐµÐ¸Ð½Ð°Ð¼ Ð½Ð¸Ð·ÐºÐ¾Ð¹ Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÐ¸. ÐÐ½ÑÐ¸ÑÐµÐ»Ð° Ðº Ð³Ð¸Ð¿Ð¾ÑÐ»Ð¾ÑÐ¸Ñ-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¼ Ð»Ð¸Ð¿Ð¾Ð¿ÑÐ¾ÑÐµÐ¸Ð½Ð°Ð¼ Ð½Ð¸Ð·ÐºÐ¾Ð¹ Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÐ¸ Ð±ÑÐ»Ð¸ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ñ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð°ÑÑÐ¸Ð½Ð½Ð¾Ð¹ ÑÑÐ¾Ð¼Ð°ÑÐ¾Ð³ÑÐ°ÑÐ¸Ð¸. ÐÐµÑÐ¾Ð´Ð¾Ð¼ ÐºÐ¾Ð½ÐºÑÑÐµÐ½ÑÐ½Ð¾Ð³Ð¾ Ð¸Ð¼Ð¼ÑÐ½Ð¾ÑÐµÑÐ¼ÐµÐ½ÑÐ½Ð¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð´Ð¾ÐºÐ°Ð·Ð°Ð»Ð¸ ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐ½Ð¾ÑÑÑ ÑÐ²ÑÐ·ÑÐ²Ð°Ð½Ð¸Ñ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½ÑÑ Ð°Ð½ÑÐ¸ÑÐµÐ» Ñ ÑÐ¾Ð±ÑÑÐ²ÐµÐ½Ð½ÑÐ¼ Ð°Ð½ÑÐ¸Ð³ÐµÐ½Ð¾Ð¼ (Ð³Ð¸Ð¿Ð¾ÑÐ»Ð¾ÑÐ¸Ñ-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¼Ð¸ Ð»Ð¸Ð¿Ð¾Ð¿ÑÐ¾ÑÐµÐ¸Ð½Ð°Ð¼Ð¸ Ð½Ð¸Ð·ÐºÐ¾Ð¹ Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÐ¸). ÐÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð° ÐºÐ¾Ð½ÑÑÐ°Ð½ÑÐ° Ð´Ð¸ÑÑÐ¾ÑÐ¸Ð°ÑÐ¸Ð¸ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ° Ð°Ð½ÑÐ¸Ð³ÐµÐ½-Ð°Ð½ÑÐ¸ÑÐµÐ»Ð¾ Ð´Ð»Ñ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½ÑÑ Ð°Ð½ÑÐ¸ÑÐµÐ». ÐÐ±Ð½Ð°ÑÑÐ¶ÐµÐ½Ñ Ð´Ð²Ðµ Ð³ÑÑÐ¿Ð¿Ñ Ð°Ð½ÑÐ¸ÑÐµÐ» Ñ ÑÐ°Ð·Ð»Ð¸ÑÐ½Ð¾Ð¹ ÑÑÐµÐ¿ÐµÐ½ÑÑ Ð°ÑÑÐ¸Ð½Ð½Ð¾ÑÑÐ¸. Ð Ð°Ð±Ð¾ÑÐ° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ Ð»Ð¸Ð¿Ð¾Ð¿ÑÐ¾ÑÐµÐ¸Ð½Ð¾Ð² Ð¸Ð¼ÐµÐ½Ð¸ Ð°ÐºÐ°Ð´ÐµÐ¼Ð¸ÐºÐ° Ð ÐÐÐ Ð. Ð. ÐÐ»Ð¸Ð¼Ð¾Ð²Ð° Ð¾ÑÐ´ÐµÐ»Ð° Ð±Ð¸Ð¾ÑÐ¸Ð¼Ð¸Ð¸ Ð¤ÐÐÐÐ£ Â«ÐÐ½ÑÑÐ¸ÑÑÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð¼ÐµÐ´Ð¸ÑÐ¸Ð½ÑÂ». ÐÑÐ¸ Ð½Ð°Ð¿Ð¸ÑÐ°Ð½Ð¸Ð¸ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±Ð·Ð¾ÑÐ° Ð±ÑÐ»Ð¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ: NCBI (PubMed), eLIBRARY.RU. Ð¡ÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÑÑ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»Ð¸ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿Ð°ÐºÐµÑÐ° Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼ Microsoft Office (Microsoft Excel).
description: This work is devoted to the isolation and study of the characteristics of human serum antibodies to low-density lipoproteins modified with hypochlorite. Tasks that were solved during the study: 1. Analyze literature sources that discuss the mechanism of chemical modification of LDL in vivo and the role of modified LDL in atherogenesis; 2. Detect antibodies to hypochlorite-modified LDL in human sera; 3. Improve the method for isolating antibodies to HOCl-LDL from human serum; 4. Prove the specificity of the isolated antibodies to HOCl-LDL; 5. Determine the dissociation constant of the antigen-antibody complex for the isolated antibodies to HOCl-LDL. Object of study: human blood serum, low-density lipoproteins, human serum albumin. During the work, antibodies to hypochlorite-modified low-density lipoproteins were detected in human blood sera. Antibodies to hypochlorite-modified low-density lipoproteins were isolated by affinity chromatography. Using the competitive enzyme immunoassay method, the specificity of binding of isolated antibodies to their own antigen (hypochlorite-modified low-density lipoproteins) was proven. The dissociation constant of the antigen-antibody complex for the isolated antibodies was determined. Two groups of antibodies with different degrees of affinity were discovered. This work was carried out based on the Laboratory of Lipoproteins named after Academician of the Russian Academy of Medical Sciences A.N. Klimov, Department of Biochemistry, Institute of Experimental Medicine. When writing the literature review, the following databases were used: NCBI (PubMed), eLIBRARY.RU. Statistical processing of experimental data was carried out using the Microsoft Office software package (Microsoft Excel).