title: ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ ÑÐ¾ÑÑÐ°Ð²Ð° Ð¿ÐµÑÐ¸ÑÐµÑÐ¸ÑÐµÑÐºÐ¾Ð¹ ÐºÑÐ¾Ð²Ð¸ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð´ÐµÑÐµÐºÑÐ¾ÑÐ° ÑÑÐ±Ð¿Ð¾Ð¿ÑÐ»ÑÑÐ¸Ð¹ ÐºÐ»ÐµÑÐ¾Ðº (ÐÐ¡ÐÐ¤-01) Ð¸ Ð¿ÑÐ¾ÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐ¸ÑÐ¾Ð¼ÐµÑÑÐ¸Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Study of the cellular composition of peripheral blood using a cell subpopulation detector (dskfâ01) and flow cytometry
Creators: name: Ð¥ÑÑÑÐ°Ð¼Ð¾Ð², ÐÐ¾Ð±Ð¾Ð¼ÑÑÐ¾Ð´
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: ÑÐ¾ÑÑÐ°Ð² Ð¿ÐµÑÐ¸ÑÐµÑÐ¸ÑÐµÑÐºÐ¾Ð¹ ÐºÑÐ¾Ð²Ð¸
Ð¿ÑÐ¾ÑÐ¾ÑÐ½Ð°Ñ ÑÐ¸ÑÐ¾Ð¼ÐµÑÑÐ¸Ð¸Ñ
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ÑÑÐ±Ð¿Ð¾Ð¿ÑÐ»ÑÑÐ¸Ð¸ ÐºÐ»ÐµÑÐ¾Ðº ÐºÑÐ¾Ð²Ð¸
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Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐ° ÐºÐ»ÐµÑÐ¾Ðº ÐºÑÐ¾Ð²Ð¸
peripheral blood composition
flow cytometry
blood cell analysis
blood cell subpopulations
cell analysis methods
immunological method
cell research
diagnostics of blood cells

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description: This work is devoted to a comparative analysis of peripheral blood studies using a cell subpopulation detector built according to the scheme of a digital microscope with laser excitation of luminescence (DSKF) and a flow cytometer. The study was conducted within the framework of determining the most costand quality-appropriate study for use in mid-level clinical laboratories. The quantitative and qualitative composition of the main blood cells was studied, as well as the study of cells that have entered apoptosis. The results obtained make it possible to objectively compare both methods and evaluate their capabilities within the framework of clinical laboratories of medical institutions. A comparative study of methods for assessing the stages of apoptosis using fluorescent markers DIOC3(6), rhodamine 123, YoPro1, Syto16, on cell lines U937, HL60, THP1 using flow cytometry and the "image cytometry" method was carried out. It is established that the "image cytometry" method implemented in the device The Russian-made DSKF01 is an affordable high-quality alternative to the method of classical flow cytometry.