Bolla (Stolla) vena Zhang & Cong & Grishin 2023, new species
Creators: name: Zhang, Jing
name: Cong, Qian
name: Grishin, Nick V.
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Lepidoptera
Hesperiidae
Bolla
Bolla vena

description: Bolla (Stolla) vena Grishin, new species   
https://zoobank.org/ F6F25879-BE84-46BB-9450-0F9A35F55461  
(Fig. 3 part, 73–74, 291–292)  
 Definition and diagnosis. In addition to the three species in the Bolla zorilla (Plötz, 1886) (type locality in Panama) complex, genomic trees reveal a fourth (and new) species from Venezuela (Fig. 3): it differs from its sister Bolla hazelae (Hayward, 1940) (type locality Ecuador: Mapoto) by 4.0% (26 bp). This new species keys to B. zorilla (E.31.21) in Evans (1953) and differs from its relatives by the following combination of characters: hindwing with a more scalloped outer margin, convex in the middle and noticeably concave towards tornus, a hyaline subapical spot in cell R 5 -M 1 is smaller than the spot in cell R 3 -R 4 (spots subequal in other species) (Fig. 73–74), ampulla with a thin style directed dorsad, harpe broader in lateral view, less extended distad, with a more prominent expansion directed dorsad, dorsal margin between the dorsal-most points of harpe and costa deeper concave (with a style in the middle) (Fig. 291–292). Due to the cryptic nature of this species, most reliable identification is achieved by DNA and a combination of the following base pairs is diagnostic in the nuclear genome: aly1281.15.8:C123T, aly813.2.4:G44C, aly727.20.3:C69T, aly 1329.6.5:G105A, aly 1329.6.5:G108T, and COI barcode: A202T, C235T, T334G, T535C, A622G.   
 Barcode sequence of the holotype. Sample NVG-18049H06, GenBank OR837655, 658 base pairs: AACTTTATATTTTATTTTTGGTATTTGATCTGGTATAGTAGGAACTTCTTTAAGAATACTTATTCGTTCAGAATTAGGAACCCCTGGATCTTTAATT GGAGATGATCAAATTTATAATACTATTGTAACAGCTCATGCTTTCATTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAATT GATTAGTTCCATTAATATTAGGAGCTCCTGATATAGCTTTTCCTCGAATAAATAATATAAGATTTTGACTTTTACCCCCTTCTTTAATACTTTTAAT TTCTAGAAGTGTAGTAGAAAATGGAGCAGGTACAGGATGAACGGTTTACCCCCCCCTTTCAGCTAATATTGCTCATCAAGGATCATCTGTAGATTTA GCTATTTTTTCCCTTCATTTAGCAGGTATTTCTTCAATTTTAGGGGCAATTAATTTTATCACAACTATTATTAATATACGAATTAACAACTTATCAT TTGATCAAATACCTTTATTTGTATGAGCAGTTGGTATCACCGCTTTACTCTTATTATTATCTTTACCTGTATTAGCAGGAGCTATTACAATACTTCT AACAGATCGAAATTTAAATACTTCATTCTTTGACCCTGCGGGAGGAGGAGATCCTATTTTATATCAACATTTATTT   
 Type material. Holotype: ♂ deposited in the National Museum of Natural History, Smithsonian Institution, Washington, DC, USA (USNM), illustrated in Fig. 73–74, bears the following six rectangular labels, five white: [VENEZUELA-ARAGUA | Rancho Grande 1100m | 18 May ‘85 | S. S. Nicolay], [Bolla ♂ | zorilla | Det. | S.S. Nicolay], [Bolla | zorilla ♂ | (Ploetz) | det. H. A. Freeman], [DNA sample ID: | NVG-18049H06 | c/o Nick V. Grishin], [USNMENT | {QR Code} | 01466668], and one red [HOLOTYPE ♂ | Bolla vena | Grishin]. Paratype: 1♀ NVG-18049F01, USNMENT_01466639 the same data as the holotype, but collected on 7-Jun-1985.   
 Type locality. Venezuela: Aragua, Rancho Grande, elevation 1100 m.   
 Etymology. The name is given for Ven [ezuel] a and is a noun in apposition.   
 Distribution. Venezuela.
description: Published as part of Zhang, Jing, Cong, Qian & Grishin, Nick V., 2023, Supplementary Materials and Appendix, pp. 1-115 in Insecta Mundi 2023 (26) on page 33, DOI: 10.5281/zenodo.10396362