cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Epigenome Interactions In Complex Neurogenetic Disorders
Creators: name: Chandramohan, Dhruva
Publisher: name: Zenodo
Year: 2015
Subjects: RNA-seq
DNA-Methylation
Neural Tube Defects
Folic Acid

description: Folic acid (FA) supplementation has been shown to decrease the occurrence of neural tube defects (NTDs) in humans. Two allelic mutations in the WNT signaling pathway gene, low density lipoprotein receptor 6 (Lrp6), provide mouse models of neural tube defects (NTDs) with opposing responses to folic acid (FA) supplementation; a high FA diet decreases incidence of NTDs in Crooked Tail (Lrp6Cd/Cd) mice, while increasing NTD occurrence in Lrp6?/? knockout mice. We gathered gene expression (RNA-seq) and DNA methylation (Enhanced-reduced representation bisulfite sequence, or ERRBS) data from two different stages in development of these two strains to investigate the mechanisms of FA rescue, and compared results to WGS and RRBS data from a human cohort of spina bifida patients. Analysis of RNA-seq from embryonic day 9.5 (E9.5) mice found more than 400 genes that were affected by dietary FA levels consistently over all conditions in both strains. Expression of the majority of those genes was suppressed by FA, with an enrichment among genes affecting nuclear processes such as histone and chromatin modification, as well as DNA methylation. Human orthologs of those genes, taken as a set, were significantly enriched for rare (minor allele frequency<0.05) SNPs and indels in the human cohort of 248 patients compared to 380 controls (q-value 3.49E-39, logistic regression). Sixteen (16) of those genes affected by dietary FA levels in mice were also enriched for genetic variants in humans (q-value <0.05, logistic regression) when considered individually, and are thus potentially implicated in the action of FA in humans. Analysis of ERRBS data from postnatal day 2 (P2) animals indicated genome wide effects of both FA supplementation and the two Lrp6 mutant alleles, with 6914 sites being consistently differentially methylated as a result of FA supplementation. The ten (10) genes that were differentially expressed in the RNAseq data according to FA levels in the embryonic animals also showed differential methylation in their promoter regions based on eRRBS at P2. Both Lrp6Cd and Lrp6? alleles were accompanied by pronounced hypermethylation on a control (2ppm) FA diet. On an elevated (10ppm) FA diet, the number of hypermethylated sites increased in the Lrp6+/? mice, while the effect in the Lrp6Cd/+ mice was reversed, inducing a strong hypomethylation signature. Thus, the mouse datasets show epigenetic signatures related to interactions among the Lrp6 locus, genetic background and FA. Upon further validation, the potential orthologus targets that we have identified in humans may allow identification of genetic and epigenetic signatures predicting risk and likely responsiveness to NTD prevention strategies.
Area Home Help and Home Care Staff Use of Time, 1978
Creators: name: Lothian Region Regional Council, Department Of Social Work
Publisher: name: UK Data Service
Year: 1981

description: This study aimed to explore the time spent on various types of activity by Area Team based Home Help/Home Care Organisers, supervisors and clerical staff. There was concern about the proportion of time available to organisers and supervisors for visiting clients. Client visits are for the purpose of assessment and review.
Roman Catholic Opinion, 1978
Creators: name: Hornsby-Smith, M. P.
name: Lee, R. M.
Publisher: name: UK Data Service
Year: 1982

description: 1. To provide a descriptive account of the social and demographic characteristics of adult Roman Catholics in England and Wales;
 2. To describe the religious attitudes, beliefs and practices of Roman Catholics, including their attitudes to recent changes in the Church;
 3. To describe the associational involvement of Roman Catholics, their friendship and marriage patterns;
 4. To test hypotheses relating to the effectiveness of Catholic schools in terms of the religious attitudes, beliefs, behaviour and commitment of their former pupils, and in a way which enabled comparisons to be made with the two studies by Greeley in the United States.
 In the analysis of the data, attempts were made to explain changes in the nature of Roman Catholicism in England and Wales in the light of both external social change and recent reforms in the Roman Catholic Church.
Integrating Gene Synthesis And Microfluidics For Rapid Protein Engineering
Creators: name: Blackburn, Matthew
Publisher: name: Zenodo
Year: 2015
Subjects: protein engineering
zinc finger protein
microfluidics
gene assembly
transcription factors

description: The ability to rapidly design proteins with novel functions will have a significant impact on medicine, biotechnology, and synthetic biology. While synthetic genes are becoming a commodity, integrated approaches that take full advantage of gene synthesis have yet to be produced. We developed a bench-top, solid-phase gene synthesis method based on asymmetric primer extension (APE) and demonstrated that linear templates from this technique can be used directly for high-throughput, on-chip protein expression, purification, and quantitative characterization using MITOMI (mechanically induced trapping of molecular interactions). Gene synthesis by APE is a restriction enzyme- and ligase-free method that operates without the need for transformation, sequencing, or PCR/oligomer purification steps. By coupling APE with cell-free protein expression and MITOMI (APE-MITOMI), we completely circumvent any molecular cloning and cell-based steps, reducing the time between gene design and protein characterization to 3-4 days per prototyping cycle. With APE-MITOMI we synthesized and characterized over 400 Cys2His2 zinc-finger (ZF) transcription factors (TF), showing that although ZF TFs can be readily engineered to recognize a particular target DNA sequence, rationally engineering the precise binding energy landscape remains difficult. We also show that it is possible to tune ZF – DNA affinity precisely and independently of sequence specificity. The APE gene synthesis method can be readily automated on standard liquid handling robots, making it possible to characterize hundreds to thousands of synthetic proteins per week. APE-MITOMI is a generic approach that should facilitate fundamental studies in protein biophysics and protein design/engineering, and by enabling a detailed exploration of the protein sequence-function relationship, improve the accuracy of computational models of protein function.
Interstellar Sodium and Calcium Absorption toward SN 2011dh in M51
Creators: name: Ritchey, Adam M.
name: Wallerstein, George
Publisher: name: arXiv
Year: 2012
Subjects: subject: Astrophysics of Galaxies (astro-ph.GA)
subject: Cosmology and Nongalactic Astrophysics (astro-ph.CO)
subject: FOS: Physical sciences

description: We present high-resolution echelle observations of SN 2011dh, which exploded in the nearby, nearly face-on spiral galaxy M51. Our data, acquired on three nights when the supernova was near maximum brightness, reveal multiple absorption components in Na I D and Ca II H and K, which we identify with gaseous material in the Galactic disk or low halo and in the disk and halo of M51. The M51 components span a velocity range of over 140 km s^-1, extending well beyond the range exhibited by H I 21 cm emission at the position of the supernova. Since none of the prominent Na I or Ca II components appear to coincide with the peak in H I emission, the supernova may lie just in front of the bulk of the H I disk. The Na I/Ca II ratios for the components with the most extreme positive and negative velocities relative to the disk are ~1.0, similar to those for more quiescent components, suggesting that the absorption originates in relatively cool gas. Production scenarios involving a galactic fountain and/or tidal interactions between M51 and its companion would be consistent with these results. The overall weakness of Na I D absorption in the direction of SN 2011dh confirms a low foreground and host galaxy extinction for the supernova.
description: 6 pages, 3 figures, emulateapj style, accepted for publication in ApJ Letters
Macro-Economic Role of Military Expenditure in OECD Countries, 1953-1973
Creators: name: Smith, R. P.
Publisher: name: UK Data Service
Year: 1982

description: A consistent set of economic time series for 12 variables and 14 OECD countries for the years 1953-1973 were assembled to test various hypotheses about the macroeconomic role of military expenditure.
Binding Studies Of Type I, Ii, And Iv Kinase Inhibitors Against  Abl Kinase Using Back-Scattering Interferometry Moa For Allosteric Inhibitors That Overcome Drug Resistance
Creators: name: Lavieri, Robert R.
Publisher: name: Zenodo
Year: 2015
Subjects: Back-scattering interferometry
Binding
Bcr-Abl

description: When abnormally expressed or controlled, kinase activity can cause cellular dysregulation and contribute to the onset of several diseases, including cancer. Based on the understanding of kinase malfunction, the discovery of small organic molecules to alter kinase function has culminated in the development of targeted cancer therapy. However, limited selectivity and the emergence of drug resistance remain fundamental challenges. Most known kinase inhibitors are Type I inhibitors, ATP-competitive compounds such as dasatinib that bind to the ATP binding site. Type II inhibitors are compounds which bind partially in the ATP binding site and extend into an adjacent allosteric site that is present only in the inactive kinase conformation. Compared to Type I inhibitors, Type II inhibitors have been shown to possess advantageous pharmacological properties. As such, many Type II inhibitors currently on the market, such as imatinib, are very effective anti-cancer drugs. Mutations resistant to Type I/II inhibitors are emerging at a rapid pace and often limit the success of targeted cancer therapies. At present, there are more than 50 mutation sites conferring different levels of imatinib resistance. Recently, a number of Type IV inhibitors that function via allosteric modulation have demonstrated promise towards addressing mutation dependent drug resistance. As such, the need to identify and develop reversible inhibitors that are resistant to such mutations and bind with a high affinity is the focus of many research projects. The binding affinity of Type I, II and IV Bcr-Abl kinase inhibitors with wild type and four mutant Bcr-Abl kinases (H396P, M351T, Q252H, and T315I) were measured using TruBind? Back-Scattering Interferometry (BSI). BSI successfully demonstrated facile determination of equilibrium dissociation constants (Kd) for all systems, with a high degree of concordance with competition assay derived IC50 results. These results indicate that BSI binding studies both class I, II, and IIV kinase inhibitors can easily be performed, allowing for confirmation of target engagement as well as direct binding assessment of type II and IV kinase inhibitors against inactive Bcr-Abl kinase. The latter makes BSI an attractive biophysical technique for the study of second and third generation kinase inhibitors to address the challenges of kinase inhibitor drug resistance.
Non-stationary compositions of Anosov diffeomorphisms
Creators: name: Stenlund, Mikko
Publisher: name: arXiv
Year: 2011
Subjects: subject: Dynamical Systems (math.DS)
subject: Mathematical Physics (math-ph)
subject: Probability (math.PR)
subject: FOS: Mathematics
subject: FOS: Physical sciences
subject: 37D20, 60F05, 82C05

description: Motivated by non-equilibrium phenomena in nature, we study dynamical systems whose time-evolution is determined by non-stationary compositions of chaotic maps. The constituent maps are topologically transitive Anosov diffeomorphisms on a 2-dimensional compact Riemannian manifold, which are allowed to change with time - slowly, but in a rather arbitrary fashion. In particular, such systems admit no invariant measure. By constructing a coupling, we prove that any two sufficiently regular distributions of the initial state converge exponentially with time. Thus, a system of the kind loses memory of its statistical history rapidly.
Reading Marriage Survey, 1974
Creators: name: Coleman, D. A.
Publisher: name: UK Data Service
Year: 1982

description: To describe and analyse geographical and social aspects of marital choice in modern Britain and to investigate their likely genetical import. Marital choice is important biologically because partners with remote origins tend to be genetically more different than those with close origins. Proximity of partners may thus affect genetical variance, the size and ease of definition of breeding populations and ultimately evolution. This research aimed to fulfil the need for a study of modern urban marriage in a way which facilitated analysis of social factors affecting choice and genetical inference from the demographic data.
High Throughput Screening Of Genetically Encoded Substance Libraries
Creators: name: Held, Martin
Publisher: name: Zenodo
Year: 2015
Subjects: Screening
bioactivity
libraries

description: We present a high throughput technology for screening of genetically encoded substances libraries. At the heart of the technology are nL-sized translucent gel-like carriers (?nano-liter reactors? or ?NLRs?) serving as growth compartments for a sensor strain used as a disease model (in our case Micrococcus flavus) and a genetically encoded library (here Lactococcus lactis secreting structurally diverse peptides). Each of NLRs is inoculated via Poisson dilution by approx. 200 cells of the sensor strain and on average one cell of the library. Both, sensor (green) and library (red) cells synthesize a specific fluorescent protein which allows estimation of the amount of sensor and library cells by large particle flow-cytometry. After inoculation, the NLRs are soaked with a growth medium and cultivated in suspensions arrays employing an organic solvent phase as the carrier liquid. The potency of the secreted substance is then determined on the basis of the fluorescence signals whereby a high to modest red paired with a low green signal intensity indicates bioactivity. We will provide an overview of the technical elements of the platform and present the first results of a screening a lantibiotic peptide library ? a substance class featuring a considerable degree of three-dimensionality and larger numbers of bioactive members.
title: Chronic glenohumeral dysplasia secondary to brachial plexus birth palsy
Creators: name: Vieira, João
Publisher: name: EURORAD
Year: 2023
Subjects: subject: Paediatric radiology
subject: Anatomy
subject: Musculoskeletal joint
subject: Musculoskeletal system
subject: Paediatric
subject: MR
subject: Clinical Case

description: Clinical History: Fifteen-year-old male with upper right limb dysfunction since birth, from vaginal delivery. He had deficit on active external rotation but preserved mobility.

description: 15 years, male
12 Years of Stellar Activity Observations in Argentina
Creators: name: Mauas, Pablo J. D.
name: Buccino, A.
name: Diaz, R.
name: Vieytes, M.
name: Petrucci, R.
name: Jofre, E.
name: Abrevaya, X.
name: Luoni, M. L.
name: Valenzuela, P.
Publisher: name: arXiv
Year: 2012
Subjects: subject: Solar and Stellar Astrophysics (astro-ph.SR)
subject: FOS: Physical sciences

description: We present an observational program we started in 1999, to systematically obtain mid-resolution spectra of late-type stars, to study in particular chromospheric activity. In particular, we found cyclic activity in four dM stars, including Prox-Cen. We directly derived the conversion factor that translates the known S index to flux in the Ca II cores, and extend its calibration to a wider spectral range. We investigated the relation between the activity measurements in the calcium and hydrogen lines, and found that the usual correlation observed is the product of the dependence of each flux on stellar color, and it is not always preserved when simultaneous observations of a particular star are considered. We also used our observations to model the chromospheres of stars of different spectral types and activity levels, and found that the integrated chromospheric radiative losses, normalized to the surface luminosity, show a unique trend for G and K dwarfs when plotted against the S index.
description: 7 pges. Accepted for publicaction in Procceedings IAU Symposium 286
The Schrödinger-Newton equation as non-relativistic limit of self-gravitating Klein-Gordon and Dirac fields
Creators: name: Giulini, Domenico
name: Großardt, André
Publisher: name: arXiv
Year: 2012
Subjects: subject: General Relativity and Quantum Cosmology (gr-qc)
subject: Quantum Physics (quant-ph)
subject: FOS: Physical sciences
subject: 35Q40

description: In this paper we show that the Schrödinger-Newton equation for spherically symmetric gravitational fields can be derived in a WKB-like expansion in 1/c from the Einstein-Klein-Gordon and Einstein-Dirac system.
description: 27 pages
Marketing Geography Computerized Bibliography, 1981
Creators: name: Dawson, J. A.
Publisher: name: UK Data Service
Year: 1982

description: 1. To provide simple, portable software to select bibliographic material, particularly geographical material.
 2. To provide software to allow user preparation of reading lists and specific bibliographies.
 3. To provide software which will allow production of general bibliographies and will allow extraction from the data of highly specific reference lists.
 4. To provide an easily updated and extended bibliographic data base structure.
 5. To provide a bibliography of use to students in marketing geography.
Measurement of polarization observables in omega-photoproduction
Creators: name: Eberhardt, Holger
Publisher: name: arXiv
Year: 2011
Subjects: subject: Nuclear Experiment (nucl-ex)
subject: FOS: Physical sciences

description: Near threshold omega meson photoproduction is studied with the CBELSA/TAPS experiment at the ELSA accelerator of Bonn University. Single as well as double polarization observables are measured using either linear or circular polarized photon beams and a longitudinal polarized proton target.
description: Proceeding for NSTAR 2011 conference
Heterobimetallic reactions involving hydride and organosulfur ligands : synthesis of heterobimetal
Creators: name: Freeman, Gavin Kerry Wyllie
Publisher: name: University of Calgary
Year: 1990
Subjects: subject: QD 474 F73 1990
subject: Catalysis
subject: Complex compounds
subject: Ligands

description: Bibliography: p. 248-266.
description: offsite
Tetraiodothyroacetic Acid-Conjugated Polyethylenimine For Enhanced  Delivery Of Plasmid Dna Via Integrin Receptor
Creators: name: Dehshahri, Ali
Publisher: name: Zenodo
Year: 2015
Subjects: polyethylenimine
gene delivery
integrin receptor

description: Targeted delivery of polymer-based nanoparticles has been considered as an efficient approach to transfer genetic materials such as plasmid DNA and siRNA. Given the presence of the binding site for tetraiodothyroacetic acid on integrin receptor and overexpression of integrin ?V?3 receptor on tumor cells, early escape from endosomal compartment by the proton sponge effect of PEI and entry of tetraiodothyroacetic acid conjugated nanoparticles into cell nucleus by TR receptors, we hypothesized that conjugation of tetraiodothyroacetic acid with PEI may overcome three major barriers considered as the main obstacles in polycation-based gene delivery. In order to test the hypothesis, modified polymer/plasmid DNA complexes were prepared and their ability in transferring plasmid encoding Interleukin-12 was investigated. Moreover, the conjugates were characterized with respect to plasmid DNA condensation ability, particle size and zeta potential as well as cell-induced toxicity and plasmid protection against DNase degradation. The results demonstrated that tetraiodothyroacetic acid derivatives of PEI were able to condense plasmid encoding IL-12 gene at a carrier to plasmid DNA (C/P) weight ratio ? 2:1. The measured particle size was around 180 nm. The highest level of IL-12 gene expression was achieved by the nanoparticles prepared by modified PEI at carrier to plasmid ratio of 8 where they could increase the level of gene expression up to 4 times compared to that of naked plasmid. The cell viability in such C/P ratios is up to 85%. These results suggest that tetraiodothyroacetic acid conjugation of PEI is a simple modification strategy for future investigations aimed at developing a targeting gene vehicle.
Consensus of Multi-Agent Systems with General Linear and Lipschitz Nonlinear Dynamics Using Distributed Adaptive Protocols
Creators: name: Li, Zhongkui
name: Ren, Wei
name: Liu, Xiangdong
name: Fu, Mengyin
Publisher: name: arXiv
Year: 2011
Subjects: subject: Systems and Control (eess.SY)
subject: Optimization and Control (math.OC)
subject: FOS: Electrical engineering, electronic engineering, information engineering
subject: FOS: Mathematics

description: This paper considers the distributed consensus problems for multi-agent systems with general linear and Lipschitz nonlinear dynamics. Distributed relative-state consensus protocols with an adaptive law for adjusting the coupling weights between neighboring agents are designed for both the linear and nonlinear cases, under which consensus is reached for all undirected connected communication graphs. Extensions to the case with a leader-follower communication graph are further studied. In contrast to the existing results in the literature, the adaptive consensus protocols here can be implemented by each agent in a fully distributed fashion without using any global information.
description: 15 pages, 6 figures, submitted to IEEE TAC
Modified Polyamidoamine Based Nanoparticles For Tumor Cell Gene Delivery
Creators: name: Alhashemi, Samira Hossaini
Publisher: name: Zenodo
Year: 2015
Subjects: PAMAM
gene delivery

description: Polyamidoamine is a cationic dendrimer with the ability to condense plasmid DNA into nanometric particles suitable for gene transfer applications. Toxicity is one of the obstacles in wide range usage of this dendrimer in cancer gene therapy. The toxicity is mainly associated with high density of positive charge on its surface which leads to cell membrane disruption. Therefore, modification of the dendrimer backbone to reduce the positive charge might be useful in order to decrease the dendrimer cytotoxicity. To achieve this goal, a series of hydrophobic derivatives of PAMAM was prepared by reacting with ethyl, butyl, hexyl and lauryl acrylates. By these modifications the surface positive charge was modulated and the hydrophobic balance of the dendrimer was improved. DNA binding affinity test and gel retardation assay demonstrated that the modified dendrimers bind to plasmid DNA efficiently. Particle size and zeta potential measurements showed that these modified polymers are able to form nanoparticles smaller than 200 nm with reduced zeta potential compared with the unmodified PAMAM. Evaluation of cytotoxicity was carried out in HepG2, MCF7 and NIH3T3 cell lines by MTT method. The results showed that the cytotoxicity of modified dendrimers and the dendrimer/plasmid complexes (dendriplexes) reduced significantly. Therefore, it is expected that the modified dendrimers will have much potential as a good candidate for gene delivery.
Norfolk Health Care Survey, 1980
Creators: name: Bentham, C. G.
name: Haynes, R. M.
Publisher: name: UK Data Service
Year: 1982

description: The aim of this project was to clarify the effects of accessibility on the use of health services in Norfolk. It was carried out in the city of Norwich and also in villages with and without general practitioner services, both near and distant from Norwich. The aim was to investigate the reasons behind the observed effects of accessibility and to identify the characteristics of people most likely to be affected.
B-Meson and Neutrino Oscillation: A Unified Treatment
Creators: name: Kayser, Boris
Publisher: name: arXiv
Year: 2011
Subjects: subject: High Energy Physics - Phenomenology (hep-ph)
subject: High Energy Physics - Experiment (hep-ex)
subject: FOS: Physical sciences

description: We present a unified treatment of the quantum mechanics of B-factory and neutrino oscillation experiments. While our approach obtains the usual phenomenological predictions for these experiments, it does so without having to invoke perplexing Einstein-Podolsky-Rosen correlations or non-intuitive kinematical assumptions.
description: 11 pages, 2 figures; for the eConf Proceedings of the Ninth International Conference on Flavor Physics and CP Violation (FPCP 2011) Maale Hachamisha, Israel, May 23-27, 2011
The Vampire bat (desmodus rotundus): an extreme in evolutionary morphology?
Creators: name: Anderson, Beverly Adele
Publisher: name: University of Calgary
Year: 1990
Subjects: subject: QL 737 C52 A53 1990
subject: Desmodus rotundus - Morphology
subject: Vampire bats - Morphology

description: Bibliography: p. 191-200.
Genomic Sequence Analysis Of Mycobacterium Tuberculosis H37Rv And M. Tuberculosis Dr-689 Subjected To Continuous  Subculturing
Creators: name: Vera-Cabrera, Lucio
name: CARMEN A. MOLINA-TORRES
name: ROCIO ORTIZ-LOPEZ
name: RAMON BARCENAS WALLS
name: GUILLERMO GUERRERO-RAMIREZ
name: JORGE CASTRO-GARZA
name: JORGE OCAMPO-CANDIANI
Publisher: name: Zenodo
Year: 2015
Subjects: Tuberculosis
Attenuation
Virulence

description: Background: Tuberculosis is caused by the bacterium Mycobacterium tuberculosis, considered as a re-emergent infectious disease and a major public health problem affecting one third of earth’s population. The only vaccine available is BCG, an attenuated M. bovis isolate obtained by continous subculture. Results after using this vaccine on human populations are quiet variable. One of the explanations is that it is not an M. tuberculosis sensu stricto. In order to explore the biological and genetic changes produced in M. bovis that ended in BCG, we have subjected several M. tuberculosis isolates to continuous growth for several years. Here we present, the results of cytotoxicity assay on THP-1 macrophage monolayers as well as the genomic sequence analysis data after 200 subcultures of two of the, M. tuberculosis H37Rv and M. tuberculosis DR-689, a Beijing strain.

Methods: Strains were subcultured in M7H9 broth with 0.5% ox bile in 50 ml Erlenmeyer flasks and incubated at 37?C /150 rpm until observation of visible growth. To measure virulence, the ability of isolates to destroy a THP-1 macrohage monolayer was tested. The WGS was obtained by using an Illumina genome analyzer IIx. The reads were aligned to the reference genome M. tuberculosis H37Rv and a list of polymorphisms -SNPs (single nucleotide polymorphisms) and INDELs (insertions and deletions) - was obtained.

Results: Ability of subcultured isolates to produce cittotoxicity to the THP-1 macrophages was decreased almost at the level of the M. bovis BCG. As controls we used the non-subcultured isolate and M. bovis BCG. More than 100 SNP`s (that is the amount enough to consider individual isolates) between the parental and subcultured isolates were observed in each case; genetic changes affecting putative virulence genes were observed.

Conclusions: As in M. bovis BCG, we observed that continuous subculturing of M. tuberculosis induces important biological changes. Data obtained from the re-sequencing analysis support our previous results concerning the lipid patterns and cytotoxicity.

Supported by CONACYT 155892.
The dynamics of the elliptic Hill problem : Periodic orbits and stability regions
Creators: name: Voyatzis, G.
name: Gkolias, I.
name: Varvoglis, H.
Publisher: name: arXiv
Year: 2011
Subjects: subject: Earth and Planetary Astrophysics (astro-ph.EP)
subject: FOS: Physical sciences

description: The motion of a satellite around a planet can be studied by the Hill model, which is a modification of the restricted three body problem pertaining to motion of a satellite around a planet. Although the dynamics of the circular Hill model have been extensively studied in the literature, only few results about the dynamics of the elliptic model were known up to now, namely the equations of motion and few unstable families of periodic orbits. In the present study we extend these results by computing a large set of families of periodic orbits and their linear stability and classify them according to their resonance condition. Although most of them are unstable, we were able to find a considerable number of stable ones. By computing appropriate maps of dynamical stability, we study the effect of the planetary eccentricity on the stability of satellite orbits. We see that, even for large values of the planetary eccentricity, regular orbits can be found in the vicinity of stable periodic orbits. The majority of irregular orbits are escape orbits.
The late-time afterglow of the extremely energetic short burst GRB 090510 revisited
Creators: name: Guelbenzu, A. Nicuesa
name: Klose, S.
name: Kruehler, T.
name: Greiner, J.
name: Rossi, A.
name: Kann, D. A.
name: E., F. Olivares
name: Rau, A.
name: Afonso, P. M. J.
name: Elliott, J.
name: Filgas, R.
name: Yoldas, A. Kuepcue
name: McBreen, S.
name: Nardini, M.
name: Schady, P.
name: Schmidl, S.
name: Sudilovsky, V.
name: Updike, A. C.
name: Yoldas, A.
Publisher: name: arXiv
Year: 2012
Subjects: subject: High Energy Astrophysical Phenomena (astro-ph.HE)
subject: FOS: Physical sciences

description: The discovery of the short GRB 090510 has raised considerable attention mainly because it had a bright optical afterglow and it is among the most energetic events detected so far within the entire GRB population. The afterglow was observed with swift/UVOT and swift/XRT and evidence of a jet break around 1.5 ks after the burst has been reported in the literature, implying that after this break the optical and X-ray light curve should fade with the same decay slope. As noted by several authors, the post-break decay slope seen in the UVOT data is much shallower than the steep decay in the X-ray band, pointing to an excess of optical flux at late times. We reduced and analyzed new afterglow light-curve data obtained with the multichannel imager GROND. Based on the densely sampled data set obtained with GROND, we find that the optical afterglow of GRB 090510 did indeed enter a steep decay phase starting around 22 ks after the burst. During this time the GROND optical light curve is achromatic, and its slope is identical to the slope of the X-ray data. In combination with the UVOT data this implies that a second break must have occurred in the optical light curve around 22 ks post burst, which, however, has no obvious counterpart in the X-ray band, contradicting the interpretation that this could be another jet break. The GROND data provide the missing piece of evidence that the optical afterglow of GRB 090510 did follow a post-jet break evolution at late times.
description: submitted to Astronomy & Astrophysics, accepted for publication on Dec 24, 2011
Establishment Of The Culture Condition To Maintain The Primary Human Colorectal Cancer Microenvironment By Using A 3D Perfusion Bioreactor
Creators: name: Manfredonia, Celeste
Publisher: name: Zenodo
Year: 2015
Subjects: tumor engineering
immunotherapy
bioreactor
tissue engineering
regenerative medicine

description: The screening and assessment of new cancer drugs has for many years carried out on conventional 2D monolayer cell culture that cannot represent the complexity of a tissue. On the other hand, the generation of patient derived xenograft (PDX) determines the loss of human-cancer associated stroma and the interaction with murine environment. In this context, the use of a 3D in-vitro systems based on human specimens that maintain the complexity of the in-vivo tissue is needed. Bioreactors that use a pump system to perfuse media directly through a scaffold are known as perfusion bioreactor. The possibility of direct perfusion allows active delivery of nutrient in complex systems. By continuously removing spent media and replacing it with new media, nutrient levels are maintained for optimal growing conditions and cell waste product is removed to avoid toxicity. Moreover perfusion-based bioreactor reduces mass transfer limitations, particularly in the central part of the scaffold. Thus, active perfusion would benefit cellular proliferation and viability. Using colorectal cancer tissue (CRC), we hypothesize that a 3D perfused scaffold-based bioreactor culture system would preserve both the transformed epithelial (EpCAM+) and the non-transformed stromal (Vimentin+ and CD45+) components and, also, keep cells proliferating (Ki67+). We demonstrated the possibility of using a perfusion-based bioreactor system to maintain alive and proliferating freshly surgical excised CRC tissue fragments in porous 3D collagen type-1 scaffolds. After 10 days of culture the tissue partially maintained its original architecture with typical neoplastic disorganization. Phenotypic analysis confirmed that expanded tissues included epithelial, stromal and hematopoietic cells. Tumor cell proliferation, as provide by Ki67 staining, was assessed. Taken together, our results indicate that culture of primary tumor fragments within perfused bioreactors can be successfully achieved over a short-time period allowing the preservation of the diverse cellular components of the CRC specimens. These ex-vivo generated tissues might mirror features of the original tumor more effectively than 2D or 3D static cultures, and of PDX, thus possibly representing useful tools for drug testing and for the evaluation of sensitivity to chemotherapies for personalized medicine.
Theory of High-Tc Superconductivity: Accurate Predictions of Tc
Creators: name: Harshman, Dale R.
name: Fiory, Anthony T.
Publisher: name: arXiv
Year: 2012
Subjects: subject: Superconductivity (cond-mat.supr-con)
subject: FOS: Physical sciences

description: The superconducting transition temperatures of high-Tc compounds based on copper, iron, ruthenium and certain organic molecules are discovered to be dependent on bond lengths, ionic valences, and Coulomb coupling between electronic bands in adjacent, spatially separated layers [1]. Optimal transition temperature, denoted as T_c0, is given by the universal expression $k_BT_c0 = e^2 Λ/ \ellζ$; $\ell$ is the spacing between interacting charges within the layers, ζis the distance between interacting layers and Λis a universal constant, equal to about twice the reduced electron Compton wavelength (suggesting that Compton scattering plays a role in pairing). Non-optimum compounds in which sample degradation is evident typically exhibit Tc < T_c0. For the 31+ optimum compounds tested, the theoretical and experimental T_c0 agree statistically to within +/- 1.4 K. The elemental high Tc building block comprises two adjacent and spatially separated charge layers; the factor e^2/ζarises from Coulomb forces between them. The theoretical charge structure representing a room-temperature superconductor is also presented.
description: 7 pages 5 references, 6 figures 1 table
Welsh Election Study, 1979
Creators: name: Madgwick, P. J.
name: Balsom, D.
Publisher: name: UK Data Service
Year: 1981

description: The purpose of this study was to investigate specific Welsh dimensions within the general field of voting behaviour and partisanship in British politics. In addition, data was collected on exclusively Welsh issues such as the Welsh language, devolution, and aspects of national identity. This study was designed and conducted in close collaboration with the British Election Study (Essex) and the Scottish Election Study (Strathclyde).
In Vivo And In Vitro Models Of Bromodomain And Extraterminal Domain Inhibitor-Induced Intestinal Stem Cell Loss And Villous Atrophy Reveal Differential Human Vs Pre-Clinal Species Sensitivity
Creators: name: Newham, Peter
Publisher: name: Zenodo
Year: 2015

description: BET domain epigenetic reader proteins play a role in controlling the expression of genes that regulate cellular differentiation and proliferation and phenotype maintenance (e.g., cMyc) and are frequently implicated in cancer; hence BET modulators are being explored as potential therapeutics in a number of tumour settings. In addition to regulating tumour cell viability, BET inhibitors are also associated with gastrointestinal toxicity, though the molecular and cellular mechanisms behind this toxicity have yet to be elucidated. We have found that BET inhibitors induce a dose-limiting duodenal villous atrophy in vivo, accompanied by inappetance and body weight loss. Ex-vivo cultures of intestinal organoids confirm that villous atrophy occurs with multiple chemical classes of BET domain inhibitors and that their toxicity is driven by their primary pharmacology. Intriguingly, transcriptomic analysis of the intestinal atrophy occurs in the absence of a proliferative block, and in the presence of cMyc, suggesting that the intestinal effects are not mediated by cMyc inhibition as may have been assumed. We find instead that BET domain inhibitors induce a rapid loss of fast cycling intestinal stem cells, suggesting that it is a loss of stem-cell self-renewal leading to crypt loss and dose-limiting duodenal toxicity. An inter-species comparison of the in vitro miniGut toxicity data indicated that human organoids are markedly less-sensitive to BET domain inhibitors mediated toxicity compared to other preclinical species (mouse, rat and dog).
Twin matter waves for interferometry beyond the classical limit
Creators: name: Lücke, Bernd
name: Scherer, Manuel
name: Kruse, Jens
name: Pezzé, Luca
name: Deuretzbacher, Frank
name: Hyllus, Phillip
name: Topic, Oliver
name: Peise, Jan
name: Ertmer, Wolfgang
name: Arlt, Jan
name: Santos, Luis
name: Smerzi, Augusto
name: Klempt, Carsten
Publisher: name: arXiv
Year: 2012
Subjects: subject: Quantum Gases (cond-mat.quant-gas)
subject: Atomic Physics (physics.atom-ph)
subject: Quantum Physics (quant-ph)
subject: FOS: Physical sciences

description: Interferometers with atomic ensembles constitute an integral part of modern precision metrology. However, these interferometers are fundamentally restricted by the shot noise limit, which can only be overcome by creating quantum entanglement among the atoms. We used spin dynamics in Bose-Einstein condensates to create large ensembles of up to $10^4$ pair-correlated atoms with an interferometric sensitivity $-1.61^{+0.98}_{-1.1}$ dB beyond the shot noise limit. Our proof-of-principle results point the way toward a new generation of atom interferometers.
description: main text plus supporting online material: 12 pages 9 figures
British Viewers' Reactions to the `Roots' Series, 1977
Creators: name: British Broadcasting Corporation, Audience Research Department
Publisher: name: UK Data Service
Year: 1982

description: The purpose of this study was to investigate British viewers' reactions to the American television series Roots. Questionnaires about all six programmes were sent to the Viewing Panel, to be followed shortly after the series by a lengthy special questionnaire covering the series as a whole. This included a number of questions which had been asked in an American study by Professor Robinson of Cleveland State University in order to provide cross-cultural comparisons.
Rapid Monoclonality Verification Methods To Boost Cell Line Development
Creators: name: McPate, Mark
name: Muelthaler, Mio
name: Lew, Wilson
Publisher: name: Zenodo
Year: 2015
Subjects: Monoclonality
CloneSelect Imager
Fluorescence

description: Regulations for cell line development are increasingly more stringent. Providing evidence that a cell line is derived from a single cell is a crucial factor in cell line generation. Typically limited dilution or fluorescence activated cell sorting is performed to seed single cells into a well. Microscopy is then used to determine the number of cells seeded in each well and monitor cell growth. While monoclonality verification via brightfield imaging is possible, debris, dust, and air bubbles make it difficult and time consuming to identify single cells which may cause high value clones to be discarded. Here we present a fluorescent method for identifying monoclonal CHO-S cells using CellTracker Green CMDFA, a fluorescent dye used to monitor cell location. We incubated CHO-S cells with Cell Tracker Green CMDFA and performed limited dilution to seed single CHO-S cells into 96-well plates. The CloneSelect Imager was used to image CHO-S cells in brightfield and fluorescence channels. Growth curve algorithms ensure that only the high growing clones are selected, while fluorescence imaging allow to quickly identify viable colonies generated from single cells. Single cell identification with the aid of fluorescence is more conclusive and robust, helping to meet stringent regulatory demands in cell line development
Older Dwellings Survey, 1980-1981
Creators: name: Brookes, J.
name: Lewis, J.
Publisher: name: UK Data Service
Year: 1982

description: The purpose of this study was to collect information on the remaining dwellings that were built between 1889 and 1899 in a number of locations within south Wales. The selected locations (Cardiff, Pontypridd, Rhondda and Cynon) form a cross-section of the coastal plain and valleys that is representative of south Wales generally.
 An information profile on the remaining dwellings that were built between 1889 and 1899 was prepared and used to test two hypotheses:-
 1. The standard of accommodation in the leasehold dwellings is poorer than the standard of accommodation in the freehold dwellings;
 2. Housing policies have been more effective in improving the standard of accommodation in the freehold dwellings than in the leasehold dwellings.
 In conclusion, the effectiveness of housing improvement policies has been assessed.
Recombinant Anti-Idiotypic Antibodies For Antibody Drug Development
Creators: name: Ylera, Francisco
Publisher: name: Zenodo
Year: 2015
Subjects: HuCAL
anti-Id
therapeutic antibodies
PK assay
ADA assay

description: Antibody drug development projects require tools for monitoring the concentration of these drug candidates in patient samples and standards for measuring anti-drug antibodies (ADA). We have used the HuCAL® recombinant antibody technology for fast development of fully human Fab and IgG anti-idiotypic (anti-ID) antibodies binding specifically and with high affinity to well-known drugs like Herceptin®, Avastin®, Rituxan®, Remicade®, Humira® and others.

The HuCAL phage display approach allows for the generation of inhibitory and non-inhibitory anti-idiotypic antibodies as well as development of antibodies against drug-target complexes. These reagents are used as fully human reference standards in ADA assays and for the development of PK assays for analysis of non-clinical and clinical samples.
The dynamical equation of the effective gluon mass
Creators: name: Aguilar, A. C.
name: Binosi, D.
name: Papavassiliou, J.
Publisher: name: arXiv
Year: 2011
Subjects: subject: High Energy Physics - Phenomenology (hep-ph)
subject: High Energy Physics - Lattice (hep-lat)
subject: High Energy Physics - Theory (hep-th)
subject: FOS: Physical sciences

description: In this article we derive the integral equation that controls the momentum dependence of the effective gluon mass in the Landau gauge. This is accomplished by means of a well-defined separation of the corresponding "one-loop dressed" Schwinger-Dyson equation into two distinct contributions, one associated with the mass and one with the standard kinetic part of the gluon. The entire construction relies on the existence of a longitudinally coupled vertex of nonperturbative origin, which enforces gauge invariance in the presence of a dynamical mass. The specific structure of the resulting mass equation, supplemented by the additional requirement of a positive-definite gluon mass, imposes a rather stringent constraint on the derivative of the gluonic dressing function, which is comfortably satisfied by the large-volume lattice data for the gluon propagator, both for SU(2) and SU(3). The numerical treatment of the mass equation, under some simplifying assumptions, is presented for the aforementioned gauge groups, giving rise to a gluon mass that is a non-monotonic function of the momentum. Various theoretical improvements and possible future directions are briefly discussed.
description: 38 pages, 17 figures
A Method For Predicting Target Drug Efficiency In Cancer Based On The Analysis Of Signaling Pathway Activation
Creators: name: Artemov, Artem
Publisher: name: Zenodo
Year: 2015

description: A new generation of anticancer therapeutics called target drugs has quickly developed in the 21st century. These drugs are tailored to inhibit cancer cell growth, proliferation, and viability by specific interactions with one or a few target proteins. However, despite formally known molecular targets for every ?target? drug, patient response to treatment remains largely individual and unpredictable. Choosing the most effective personalized treatment remains a major challenge in oncology and is still largely trial and error. Here we present a novel approach for predicting target drug efficacy based on the gene expression signature of the individual tumor sample(s). The enclosed bioinformatic algorithm detects activation of intracellular regulatory pathways in the tumor in comparison to the corresponding normal tissues. According to the nature of the molecular targets of a drug, it predicts whether the drug can prevent cancer growth and survival in each individual case by blocking the abnormally activated tumor-promoting pathways or by reinforcing internal tumor suppressor cascades. To validate the method, we compared the distribution of predicted drug efficacy scores for five drugs (Sorafenib, Bevacizumab, Cetuximab, Sorafenib, Imatinib, Sunitinib) and seven cancer types (Clear Cell Renal Cell Carcinoma, Colon cancer, Lung adenocarcinoma, non-Hodgkin Lymphoma, Thyroid cancer and Sarcoma) with the available clinical trials data for the respective cancer types and drugs. The percent of responders to a drug treatment correlated significantly (Pearson?s correlation 0.77 p=0.023) with the percent of tumors showing high drug scores calculated with the current algorithm.