title: ÐÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ð¹ Ð¼ÐµÐ¶Ð´Ñ Ð±ÐµÐ»ÐºÐ°Ð¼Ð¸ Ð´ÐµÐ»ÐµÐ½Ð¸Ñ Acholeplasma laidlawii Ð¸ Mycoplasma gallisepticum Ð¿ÑÐ¸ Ð¿Ð¾Ð¼Ð¾ÑÐ¸ Ð´Ð²ÑÐ³Ð¸Ð±ÑÐ¸Ð´Ð½Ð¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Determination of interactions between division proteins Acholeplasma laidlawii and Mycoplasma gallisepticum using a two-hybrid system
Creators: name: Ð¥Ð°ÑÐ°Ð½Ð¾Ð²Ð°, ÐÐ¹Ð·Ð¸Ð»Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: FtsZ
Acholeplasma laidlawii
Mycoplasma galisepticum
Mollicutes
ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ðµ Ð´ÐµÐ»ÐµÐ½Ð¸Ðµ
Ð±ÐµÐ»ÐºÐ¸ Ð´ÐµÐ»ÐµÐ½Ð¸Ñ
cell division
division proteins

description: Ð Ð°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ð¹ Ð¼ÐµÐ¶Ð´Ñ Ð±ÐµÐ»ÐºÐ°Ð¼Ð¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð´Ð²ÑÐ³Ð¸Ð±ÑÐ¸Ð´Ð½ÑÑ ÑÐ¸ÑÑÐµÐ¼. Ð ÑÐ°Ð±Ð¾ÑÐµ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ Ð´Ð²ÑÐ³Ð¸Ð±ÑÐ¸Ð´Ð½ÑÐµ ÑÐ¸ÑÑÐµÐ¼Ñ, Ð¾ÑÐ½Ð¾Ð²Ð°Ð½Ð½ÑÐµ Ð»Ð¸Ð±Ð¾ Ð½Ð° Ð²Ð¾ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¸Ð¸ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÑÐµÑÐ¼ÐµÐ½ÑÐ° Ð°Ð´ÐµÐ½Ð¸Ð»Ð°ÑÑÐ¸ÐºÐ»Ð°Ð·Ñ Ñ Ð¼ÑÑÐ°Ð½ÑÐ° Escherichia coli âcya, Ð»Ð¸Ð±Ð¾ Ð½Ð° Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ð¸ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ°, ÐºÐ¾Ð³Ð´Ð° Ð´Ð²Ð° Ð±ÐµÐ»ÐºÐ°, ÑÐ»Ð¸ÑÑÐµ Ñ Ð½ÐµÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½ÑÐ¼Ð¸ ÑÑÐ°Ð³Ð¼ÐµÐ½ÑÐ°Ð¼Ð¸ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ°, Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²ÑÑÑ Ð´ÑÑÐ³ Ñ Ð´ÑÑÐ³Ð¾Ð¼. Ð Ð°ÑÑÐ¼Ð°ÑÑÐ¸Ð²Ð°Ð»Ð¸ÑÑ Ð¼ÐµÐ¶Ð±ÐµÐ»ÐºÐ¾Ð²ÑÐµ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ð±ÐµÐ»ÐºÐ¾Ð² Acholeplasma laidlawii Ð¸ Mycoplasma gallisepticum, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐ²Ð»ÑÑÑÑÑ Ð¿Ð°ÑÐ¾Ð³ÐµÐ½Ð°Ð¼Ð¸ Ð¿ÑÐ¸Ñ Ð¸ ÑÐ°ÑÑÐµÐ½Ð¸Ð¹ Ð¸ Ð¾ÑÐ½Ð¾ÑÑÑÑÑ Ðº ÐºÐ»Ð°ÑÑÑ Mollicutes (Ð¼Ð¸ÐºÐ¾Ð¿Ð»Ð°Ð·Ð¼Ñ). ÐÐ°ÑÐµÑÑÐ²ÐµÐ½Ð½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð· Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð´Ð²ÑÐ³Ð¸Ð±ÑÐ¸Ð´Ð½Ð¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ñ, Ð¾ÑÐ½Ð¾Ð²Ð°Ð½Ð½Ð¾Ð¹ Ð½Ð° Ð²Ð¾ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¸Ð¸ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð°Ð´ÐµÐ½Ð¸Ð»Ð°Ñ-ÑÐ¸ÐºÐ»Ð°Ð·Ñ, Ð¿Ð¾Ð·Ð²Ð¾Ð»Ð¸Ð» Ð¿Ð¾ÐºÐ°Ð·Ð°ÑÑ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ðµ Ð±ÐµÐ»ÐºÐ° FtsZ A. laidlawii Ñ Ð±ÐµÐ»ÐºÐ°Ð¼Ð¸ EF-Tu, FtsZ, IbpA, FtsK Ð¸ SepF ÑÑÐ¾Ð³Ð¾ Ð¶Ðµ Ð²Ð¸Ð´Ð°, Ð° ÑÐ°ÐºÐ¶Ðµ Ð±ÐµÐ»ÐºÐ° FtsZ M. gallisepticum Ñ Ð±ÐµÐ»ÐºÐ°Ð¼Ð¸ EF-Tu, FtsK, FtsZ Ð¸ GapD ÑÑÐ¾Ð³Ð¾ Ð¶Ðµ Ð²Ð¸Ð´Ð°. ÐÐ¾Ð»Ð¸ÑÐµÑÑÐ²ÐµÐ½Ð½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð· Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ ÑÐºÐ°Ð·Ð°Ð½Ð½Ð¾Ð¹ Ð²ÑÑÐµ Ð´Ð²ÑÐ³Ð¸Ð±ÑÐ¸Ð´Ð½Ð¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ñ Ð¿Ð¾Ð·Ð²Ð¾Ð»Ð¸Ð» Ð¾ÑÐµÐ½Ð¸ÑÑ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ðµ Ð±ÐµÐ»ÐºÐ° FtsZ A. laidlawii Ñ Ð±ÐµÐ»ÐºÐ°Ð¼Ð¸ EF-Tu, GapD Ð¸ FtsZ ÑÑÐ¾Ð³Ð¾ Ð¶Ðµ Ð²Ð¸Ð´Ð°. Ð¤Ð»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð°Ñ Ð´Ð²ÑÐ³Ð¸Ð±ÑÐ¸Ð´Ð½Ð°Ñ ÑÐ¸ÑÑÐµÐ¼Ð° Ð¿Ð¾Ð·Ð²Ð¾Ð»Ð¸Ð»Ð° Ð²ÑÑÐ²Ð¸ÑÑ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ðµ FtsZ Ð¸ EF-Tu M. gallisepticum, Ð° ÑÐ°ÐºÐ¶Ðµ FtsZ Ð¸ EF-Tu, FtsZ Ð¸ SepF A. laidlawii.
description: The given work is devoted to the study of interactions between bacterial proteins using two-hybrid systems. The work used two-hybrid systems based either on the restoration of the activity of the adenylate cyclase enzyme in the âcya mutant of Escherichia coli, or on the formation of a fluorescent protein when two proteins fused with non-fluorescent fragments of a fluorescent protein interact with each other. The interactions of proteins of Acholeplasma laidlawii and Mycoplasma gallisepticum, which are pathogens of birds and plants and belong to the class of Mollicutes (mycoplasmas), were considered. Qualitative analysis using a two-hybrid system based on the restoration of adenylate cyclase activity allowed us to show the interaction of the FtsZ protein of A. laidlawii with the EF-Tu, FtsZ, IbpA, FTsK and SepF proteins of the same species, as well as the FtsZ protein of M. gallisepticum with the EF-Tu, FTsK, FtsZ and GapD proteins of the same species. Quantitative analysis using the above-mentioned two-hybrid system allowed us to evaluate the interaction of the FtsZ protein of A. laidlawii with the EF-Tu, GapD and FtsZ proteins of the same species. The fluorescent two-hybrid system allowed to reveal interactions between FtsZ and EF-Tu of M. gallisepticum, as well as FtsZ and EF-Tu, FtsZ and SepF of A. laidlawii.