title: ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¾ÑÐ²ÐµÑÐ° ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ð°Ð»ÑÐ½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð½Ð° Ð»Ð¸Ð³Ð°Ð½Ð´Ñ ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° ÑÐ¿Ð¸Ð´ÐµÑÐ¼Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÐ°ÐºÑÐ¾ÑÐ° ÑÐ¾ÑÑÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Study of the physiological response of human endometrial stem cells to epidermal growth factor receptor ligands
Creators: name: ÐÐ°Ð±Ð´ÑÐ°ÑÐ¼Ð°Ð½Ð¾Ð²Ð°, ÐÑÐ»ÑÐ½Ð°ÑÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÐÐ»ÐµÑÐºÐ° (Ð±Ð¸Ð¾Ð».)
ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ð°Ð»ÑÐ½ÑÐµ Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÐµ ÑÑÑÐ¾Ð¼Ð°Ð»ÑÐ½ÑÐµ ÐºÐ»ÐµÑÐºÐ¸
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ÑÐ¿Ð¸Ð´ÐµÑÐ¼Ð°Ð»ÑÐ½ÑÐ¹ ÑÐ°ÐºÑÐ¾Ñ ÑÐ¾ÑÑÐ°
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ÑÑÐ°Ð½ÑÑÐ¾ÑÐ¼Ð¸ÑÑÑÑÐ¸Ð¹ ÑÐ°ÐºÑÐ¾Ñ ÑÐ¾ÑÑÐ°-Î±
ÐºÐ»ÐµÑÐºÐ¸ HeLa
endometrial mesenchymal stromal cells
epidermal growth factor receptors
epidermal growth factor
transforming growth factor-Î±
amphiregulin
HELA cells

description: Ð¦ÐµÐ»ÑÑ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð²Ð¾Ð·Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ð»Ð¸Ð³Ð°Ð½Ð´Ð¾Ð² ÑÐ­Ð¤Ð Â (Ð­Ð¤Ð , Ð¢Ð¤Ð âÎ±, ÐÐ ) Ð½Ð° Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ ÑÑÐ´ÑÐ±Ñ Ð»Ð¸Ð³Ð°Ð½Ð´âÑÐµÑÐµÐ¿ÑÐ¾ÑÐ½ÑÑ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ¾Ð², Ð¿ÑÐ¾Ð»Ð¸ÑÐµÑÐ°ÑÐ¸Ñ Ð¸ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÑ ÑÐ½ÐÐ¡Ð Ð² ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ð¸ Ñ ÐºÐ»ÐµÑÐºÐ°Ð¼Ð¸ Ð¾Ð¿ÑÑÐ¾Ð»ÐµÐ²Ð¾Ð³Ð¾ Ð¿ÑÐ¾Ð¸ÑÑÐ¾Ð¶Ð´ÐµÐ½Ð¸Ñ HeLa. ÐÐ»Ñ ÑÐµÑÐµÐ½Ð¸Ñ Ð·Ð°Ð´Ð°Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ Ð¼ÐµÑÐ¾Ð´Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð¹ ÐºÐ¾Ð½ÑÐ¾ÐºÐ°Ð»ÑÐ½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸, SDSâÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÐ·Ð° Ð¸ Ð¸Ð¼Ð¼ÑÐ½Ð¾Ð±Ð»Ð¾ÑÐ¸Ð½Ð³Ð°, Ð¿ÑÐ¾ÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐ¸ÑÐ¾Ð¼ÐµÑÑÐ¸Ð¸, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¸Ð¼Ð¼ÑÐ½Ð¾ÑÐµÑÐ¼ÐµÐ½ÑÐ½Ð¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð°. ÐÐ¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾, Ð² Ð¾ÑÐ»Ð¸ÑÐ¸Ðµ Ð¾Ñ Ð­Ð¤Ð  Ð¸ Ð¢Ð¤Ð âÎ±, ÐÐ  Ð½Ðµ Ð²ÑÐ·ÑÐ²Ð°ÐµÑ Ð¸Ð½ÑÐµÑÐ½Ð°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ ÑÐ­Ð¤Ð  ÐºÐ°Ðº Ð² ÑÐ½ÐÐ¡Ð, ÑÐ°Ðº Ð¸ Ð² HeLa. Ð ÑÐ½ÐÐ¡Ð Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐ° ÑÐ½Ð´Ð¾ÑÐ¸ÑÐ¾Ð·Ð° Ð¸ Ð»Ð¸Ð·Ð¾ÑÐ¾Ð¼Ð½Ð¾Ð¹ Ð´ÐµÐ³ÑÐ°Ð´Ð°ÑÐ¸Ð¸ Ð¿Ð¾Ð´ Ð´ÐµÐ¹ÑÑÐ²Ð¸ÐµÐ¼ Ð­Ð¤Ð  Ð¸ Ð¢Ð¤Ð âÎ± Ð·Ð°Ð¼ÐµÐ´Ð»ÐµÐ½Ð° Ð¿Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ Ñ HeLa. Ð¢Ð°ÐºÐ¶Ðµ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¾, ÑÑÐ¾ ÐÐ  Ð½Ðµ Ð²Ð»Ð¸ÑÐµÑ Ð½Ð° Ð¿ÑÐ¾Ð»Ð¸ÑÐµÑÐ°ÑÐ¸Ñ Ð² ÑÐ½ÐÐ¡Ð, ÑÐ¾Ð³Ð´Ð° ÐºÐ°Ðº Ð­Ð¤Ð  Ð¸ Ð¢Ð¤Ð âÎ± ÐµÐµ ÑÑÐºÐ¾ÑÑÑÑ. ÐÐ¿ÑÑÐ¾Ð»ÐµÐ²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸, Ð½Ð°Ð¿ÑÐ¾ÑÐ¸Ð², Ð±ÑÑÑÑÐµÐµ Ð¿ÑÐ¾Ð»Ð¸ÑÐµÑÐ¸ÑÑÑÑ Ð² Ð¿ÑÐ¸ÑÑÑÑÑÐ²Ð¸Ð¸ ÐÐ . ÐÑÑÐ²Ð»ÐµÐ½Ð¾, ÑÑÐ¾ ÑÐµÑÐµÐ¿ÑÐ¾Ñ-Ð¾Ð¿Ð¾ÑÑÐµÐ´ÑÐµÐ¼ÑÐµ ÑÐ¸Ð³Ð½Ð°Ð»ÑÐ½ÑÐµ Ð¿ÑÑÐ¸, ÑÑÐ¸Ð¼ÑÐ»Ð¸ÑÑÐµÐ¼ÑÐµ Ð­Ð¤Ð  Ð¸ Ð¢Ð¤Ð âÎ±, Ð¿ÑÐ¸Ð²Ð¾Ð´ÑÑ Ðº Ð¿Ð¾Ð´Ð°Ð²Ð»ÐµÐ½Ð¸Ñ Ð´ÐµÑÐ¸Ð´ÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐ¸ ÑÐ½ÐÐ¡Ð. ÐÐ½ÑÐµÑÐµÑÐ½Ð¾, ÑÑÐ¾ ÐÐ¡Ð Ð¸Ð· ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ñ Ð¸ Ð¿Ð»Ð°ÑÐµÐ½ÑÑ, Ð° ÑÐ°ÐºÐ¶Ðµ ÑÑÑÑÐ¾Ð³ÐµÐ½-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼ÑÐµ Ð¾Ð¿ÑÑÐ¾Ð»ÐµÐ²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸, Ð² Ð¾ÑÐ»Ð¸ÑÐ¸Ðµ Ð¾Ñ ÐÐ¡Ð Ð¸Ð· Ð¿ÑÐ»ÑÐ¿Ñ Ð·ÑÐ±Ð° Ð¸ ÐÐ°ÑÑÐ¾Ð½Ð¾Ð²Ð° ÑÑÑÐ´Ð½Ñ ÑÐµÐºÑÐµÑÐ¸ÑÑÑÑ ÐÐ  Ð² ÑÑÐµÐ´Ñ. Ð ÑÐ°Ð±Ð¾ÑÐµ Ð²Ð¿ÐµÑÐ²ÑÐµ Ð¾Ð¿Ð¸ÑÐ°Ð½Ð¾ ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÐºÐ°Ð½Ð¾Ð½Ð¸ÑÐµÑÐºÐ¾Ð¹ ÑÐ¸Ð³Ð½Ð°Ð»ÑÐ½Ð¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ñ ÑÐ­Ð¤Ð  Ð² ÑÐ½ÐÐ¡Ð, ÑÑÐ¾ ÑÐ¾Ð·Ð´Ð°ÐµÑ Ð±Ð¾Ð»ÐµÐµ Ð¿Ð¾Ð»Ð½Ð¾Ðµ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÐµÐ½Ð¸Ðµ ÐºÐ°Ðº Ð¾ ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ð¸ ÐºÐ»ÐµÑÐ¾Ðº, ÑÐ¿Ð¾ÑÐ¾Ð±Ð½ÑÑ Ð²Ð¾ÑÐ¿ÑÐ¸Ð½Ð¸Ð¼Ð°ÑÑ ÑÑÐ¸ ÑÐ¸Ð³Ð½Ð°Ð»Ñ Ð² ÑÐ¾Ð´Ðµ Ð¿ÑÐ¾Ð»Ð¸ÑÐµÑÐ°ÑÐ¸Ð¸ Ð¸ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐ¸. ÐÑÐ¾Ð¼Ðµ ÑÐ¾Ð³Ð¾, ÑÑÐ¸ ÐºÐ»ÐµÑÐºÐ¸ Ð¼Ð¾Ð³ÑÑ Ð±ÑÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð¼Ð¾Ð´ÐµÐ»Ð¸ ÑÐ¸Ð³Ð½Ð°Ð»ÑÐ½ÑÑ Ð¿ÑÐ¾ÑÐµÑÑÐ¾Ð², Ð¿ÑÐ¾ÑÐµÐºÐ°ÑÑÐ¸Ñ Ð² Ð½Ð¾ÑÐ¼Ð°Ð»ÑÐ½ÑÑ Ð½ÐµÑÑÐ°Ð½ÑÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ Ð² ÑÐºÐ°Ð½ÐµÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐ½Ð¾Ð¼ ÐºÐ¾Ð½ÑÐµÐºÑÑÐµ. Ð ÑÐ¾Ð²Ð¾ÐºÑÐ¿Ð½Ð¾ÑÑÐ¸ ÑÑÐ¸ Ð´Ð°Ð½Ð½ÑÐµ Ð¼Ð¾Ð³ÑÑ Ð±ÑÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ Ð´Ð»Ñ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ¸ Ð½Ð¾Ð²ÑÑ Ð¿Ð¾Ð´ÑÐ¾Ð´Ð¾Ð² Ð² ÑÐµÑÐ°Ð¿Ð¸Ð¸ Ð¿Ð°ÑÐ¾Ð»Ð¾Ð³Ð¸Ð¹ ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ñ.
description: The aim of the work was to study the physiological effect of EGFR ligandsÂ (EGF, TGFâÎ±, AR) on the intracellular fate of ligand-receptor complexes, as well as proliferation and differentiation of enMSCs in comparison with cells of tumor origin HeLa. In the study the methods of fluorescence confocal microscopy, SDS electrophoresis and immunoblotting, flow cytometry, and enzymeâlinked immunoassay were used. It was shown that, unlike EGF and TGFâÎ±, AR does not cause internalization of EGFR both in enMSCs and in HeLa. In enMSCs, the dynamics of endocytosis and lysosomal degradation under the action of EGF and TGFâÎ± is slower than in HeLa. It was also found that AR does not affect proliferation in enMSCs, while EGF and TGFâÎ± accelerate it. Tumor cells, on the contrary, proliferate faster in the presence of AR. It was found that receptor-mediated signaling pathways stimulated by EGF and TGFâÎ± lead to the suppression of decidual differentiation of enMSCs. Interestingly, MSCs from the endometrium and placenta, as well as estrogenâdependent tumor cells, unlike MSCs from the dental pulp and Wharton's jelly, secrete AR into the medium. The study describes for the first time the functioning of the canonical EGFR signaling system in enMSCs, which creates a more complete understanding of the physiology of stromal cells that are able to perceive these signals during proliferation and differentiation. In addition, these cells can be used as a model of signaling processes occurring in normal non-transformed cells in a tissueâspecific context. Together, these data can be used to develop new approaches in the treatment of endometrial pathologies.