Gint amoudensis Kovarik, Lowe, Just, Awale, Elmi & Stahlavsky 2018
Creators: name: Just, Pavel
name: Šťáhlavský, František
name: Kovařík, František
name: Štundlová, Jana
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Arachnida
Scorpiones
Buthidae
Gint
Gint amoudensis

description: GINT AMOUDENSIS
T h e m o s t s t r i k i n g i n t r a s p e c i f i c v a r i a b i l i t y i s discovered in G. amoudensis, which exhibits four cytotypes differing in the diploid number, multivalent associations and in the number and position of 18S rDNA sites. The cytotypes II, III and IV occur sympatrically, while the cytotype I (S1325) is found in a remote locality Borama c. 150 km far from the other cytotypes.
Cytotype I (1Ƌ; S1325) exhibits 2 n = 36 chromosomes, which decrease in length from 4.49% to 1.63% of DSL (Supporting Information, Fig. S2C; Table S2). The post-pachytene nuclei show 16 bivalents and one quadrivalent (chromosomes 1, 2, 9 and 15) (Fig. 3A; Supporting Information, Fig. S2C). Three 18S rDNA sites are detected (Fig. 3B), two of them are located in the subterminal region of the chromosomes 1 and 2 involved in the quadrivalent and one in the terminal position of chromosome 2 in the quadrivalent (Supporting Information, Fig. S2C).
Cytotype II (1 Ƌ; S1291) has 2 n = 35 chromosomes, which decrease in length from 5.01% to 1.45% of DSL (Supporting Information, Fig. S2D; Table S2). The post-pachytene spermatocytes exhibit 14 bivalents, one trivalent (chromosomes 4, 24 and 35) and one quadrivalent (chromosomes 1, 5, 8 and 23) (Fig. 3C; Supporting Information, Fig. S2D). Three 18S rDNA loci are present (Fig. 3D), one pair is located on the chromosomes 1 (subterminal site) and 5 (terminal site) involved in the quadrivalent, and one single heterozygous locus is situated in the interstitial region of the third largest bivalent (chromosome 9; Fig. 3D; Supporting Information, Fig. S2D).
Cytotype III (1 Ƌ; S1293) has 2 n = 36 chromosomes, which decrease in length from 4.71% to 1.52% of DSL (Supporting Information, Fig. S2E; Table S2). The post-pachytene cells show 15 bivalents and one hexavalent (chromosomes 1, 2, 5, 6, 11 and 24) (Fig. 3E; Supporting Information, Fig. S2E). One pair of 18S rDNA sites is located in the subterminal region of the chromosomes 1 and 2 of the hexavalent (Fig. 3F; Supporting Information, Fig. S2E).
Cytotype IV (1 Ƌ; S1292) exhibits 2 n = 35 chromosomes, which decrease in length from 4.66% to 1.54% of DSL (Supporting Information, Fig. S2F; Table S2). The post-pachytene nuclei show a configuration of 13 bivalents, one trivalent (chromosomes 7, 26 and 35) and one hexavalent (chromosomes 1, 4, 5, 6, 10 and 23) (Fig. 3G; Supporting Information, Fig. S2F). One pair of 18S rDNA sites is located in the subterminal region of the chromosomes 1 and 4 involved in the hexavalent (Fig. 3H; Supporting Information, Fig. S2F).
description: Published as part of Just, Pavel, Šťáhlavský, František, Kovařík, František & Štundlová, Jana, 2022, Tracking the trends of karyotype differentiation in the phylogenetic context of Gint, a scorpion genus endemic to the Horn of Africa (Scorpiones: Buthidae), pp. 885-901 in Zoological Journal of the Linnean Society 196 (2) on pages 892-893, DOI: 10.1093/zoolinnean/zlac049, http://zenodo.org/record/7184492