title: ÐÑÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ, Ð¾ÑÐ¸ÑÑÐºÐ° Ð¸ Ð¿Ð¾Ð¸ÑÐº ÑÑÐ»Ð¾Ð²Ð¸Ð¹ ÐºÑÐ¸ÑÑÐ°Ð»Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ ÑÐ¸ÑÐ¾Ð¿Ð»Ð°Ð·Ð¼Ð°ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð´Ð¾Ð¼ÐµÐ½Ð° ÑÐ¸Ð³Ð¼Ð°-1 ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Extratcion, purification and crystallization screen of human sigma-1 receptor CTD
Creators: name: ÐÐ¾Ð»Ð³Ð¾Ð², ÐÐ»ÐµÐºÑÐ°Ð½Ð´Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2019
Subjects: ÑÐ¸Ð³Ð¼Ð°-1 ÑÐµÑÐµÐ¿ÑÐ¾Ñ
ÑÐ¸ÑÐ¾Ð¿Ð»Ð°Ð·Ð¼Ð°ÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð´Ð¾Ð¼ÐµÐ½
Ð³ÐµÑÐµÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ°Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ
Ð½Ð°ÑÐ¸Ð²Ð½ÑÐ¹ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÐ·
ÑÐµÑÐ²ÐµÑÑÐ¸ÑÐ½Ð°Ñ ÑÑÑÑÐºÑÑÑÐ°
ÑÐºÑÐ¸Ð½Ð¸Ð½Ð³ ÑÑÐ»Ð¾Ð²Ð¸Ð¹ ÐºÑÐ¸ÑÑÐ°Ð»Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸
ÑÐ°Ð¹Ñ-Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð½ÑÐ¹ Ð¼ÑÑÐ°Ð³ÐµÐ½ÐµÐ·
sigma-1 receptor
CTD
heterologous expression
CN-PAGE
quarternery structure
crystallization screen
site-directed mutagenesis

description: Ð ÑÐ°Ð±Ð¾ÑÐµ Ð¸ÑÑÐ»ÐµÐ´ÑÑÑÑÑ ÑÑÐ»Ð¾Ð²Ð¸Ñ ÐºÑÐ¸ÑÑÐ°Ð»Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ ÑÐµÐºÐ¾Ð¼Ð±Ð¸Ð½Ð°Ð½ÑÐ½Ð¾Ð³Ð¾ ÑÐ¸ÑÐ¾-Ð¿Ð»Ð°Ð·Ð¼Ð°ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐ¸Ð³Ð¼Ð°-1 ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°. Ð ÑÐ¾Ð´Ðµ ÑÐ°Ð±Ð¾ÑÑ Ð³ÐµÐ½ ÑÐ¸ÑÐ¾Ð¿Ð»Ð°Ð·-Ð¼Ð°ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð´Ð¾Ð¼ÐµÐ½Ð° ÑÐ¸Ð³Ð¼Ð°-1 ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð±ÑÐ» ÐºÐ»Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½ Ð² Ð±Ð°ÐºÑÐµÑÐ¸-ÑÑ E.coli, ÑÐ¾Ð·Ð´Ð°Ð½Ð° ÑÐ¸ÑÑÐµÐ¼Ð° ÑÐ¸Ð½ÑÐµÐ·Ð° Ð¸ Ð¾ÑÐ¸ÑÑÐºÐ¸ ÑÐµÐºÐ¾Ð¼Ð±Ð¸Ð½Ð°Ð½ÑÐ½Ð¾Ð³Ð¾ ÑÐ¸ÑÐ¾Ð¿Ð»Ð°Ð·Ð¼Ð°ÑÐ¸-ÑÐµÑÐºÐ¾Ð³Ð¾ Ð´Ð¾Ð¼ÐµÐ½Ð° ÑÐ¸Ð³Ð¼Ð°-1 ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° Ð² ÑÐ¾ÑÑÐ°Ð²Ðµ Ð³Ð¸Ð±ÑÐ¸Ð´Ð½Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° Ð² ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐµ Ñ Ð¼Ð°Ð»ÑÑÐ¾Ð·Ð°-ÑÐ²ÑÐ·ÑÐ²Ð°ÑÑÐ¸Ð¼ Ð±ÐµÐ»ÐºÐ¾Ð¼. ÐÐµÑÐ¾Ð´Ð¾Ð¼ Ð±ÐµÑÑÐ²ÐµÑÐ½Ð¾Ð³Ð¾ Ð½Ð°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾-ÑÐµÐ·Ð° Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ ÑÐ°ÑÑÐ²Ð¾Ñ Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÑÐµÑ ÑÐ¾Ð±Ð¾Ð¹ Ð¿Ð¾Ð»Ð¸Ð´Ð¸Ñ-Ð¿ÐµÑÑÐ½ÑÑ ÑÐ¼ÐµÑÑ Ð¾Ð»Ð¸Ð³Ð¾Ð¼ÐµÑÐ¾Ð² Ð¸ Ð¼Ð¾Ð½Ð¾Ð¼ÐµÑÐ¾Ð². Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ñ Ð´Ð²Ðµ ÑÑÑÐ°ÑÐµÐ³Ð¸Ð¸ ÑÐµÑÐµ-Ð½Ð¸Ñ Ð¿ÑÐ¾Ð±Ð»ÐµÐ¼Ñ Ð¿Ð¾Ð»Ð¸Ð´Ð¸ÑÐ¿ÐµÑÑÐ½Ð¾ÑÑÐ¸: Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ Ð´ÐµÑÐµÑÐ³ÐµÐ½ÑÐ¾Ð² Ð¸ Ð½ÐµÐ´ÐµÑÐµÑÐ³ÐµÐ½ÑÐ½ÑÑ Ð´Ð¾Ð±Ð°Ð²Ð¾Ðº Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ðµ Ð¼ÑÑÐ°Ð½ÑÐ½ÑÑ ÑÐ¾ÑÐ¼ ÑÐ¸ÑÐ¾Ð¿Ð»Ð°Ð·Ð¼Ð°ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð´Ð¾Ð¼ÐµÐ½Ð° Ñ ÑÐ¾ÑÐµÑ-Ð½Ð¾Ð¹ Ð¼ÑÑÐ°ÑÐ¸ÐµÐ¹ Ð² ÑÐ°Ð¹ÑÐµ Ð¾Ð»Ð¸Ð³Ð¾Ð¼ÐµÑÐ¸Ð·Ð°ÑÐ¸Ð¸ GXXXG.
description: In this thesis the crystallization screening of the CTD of recombinant hu-man sigma-1 receptor CTD is carried out. The gene of human sigma-1 receptor CTD has been cloned and the CTD fused to MBP by 4 alanine amino acid se-quence has been overexpressed in E. coli bacteria. The downstream process of protein extraction and purification has been optimized. The result of CN-PAGE analysis showed that the protein of interest exists in a solution as a mixture of monomeric and homooligomeric forms. To address this issue, we undertook the studies to convert the CTD of human sigma-1 receptor to the homogenous state. These studies have shown that using different detergents and crystallization addi-tives may be one of the ways to solve this problem. The second way is to design mutant forms of sigma-1 receptor CTD by replacing one of the amino acids in GXXXG oligomerization site.