cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Controlling Interactions of High-Spin Organic Conjugates with Molecular Spacers
Creators: Schroeder, Zachary W
Publisher: name: University of Alberta Library
Year: 2024

The ability to efficiently harness and manipulate the energy cast down to earth from the sun is hampered by thermodynamics and economics. Any single photovoltaic material can only convert a narrow spectrum of light into energy efficiently. Much of the light emitted from the sun merely radiates away as heat (thermalization). Singlet fission is highly sought for its potential to address the thermalization problem in light-harvesting materials (photovoltaics). Despite more than 50 years of progress since the discovery of singlet fission, efficient photovoltaic materials have yet to be realized using the singlet fission process. To understand the complex process of singlet fission, model compounds are designed and synthesized to investigate the fundamental mechanisms of the process. In this thesis, Chapters 2–6 describe the synthesis of a series of pentacene- and anthracene-based model compounds to investigate singlet fission and its associated processes or applications. In Chapter 2, the synthesis of pentacene dimers to investigate triplet lifetimes, post singlet fission energy harvesting, and the switchability of singlet fission is described. In Chapter 3, the synthesis of acene models to investigate singlet fission induced two-electron reductions of acceptors and bright fission is described. In Chapter 4, the effects of functional groups on the stability of pentacene are investigated. Chromophores that undergo singlet fission are often kinetically unstable, and methods to rectify such stability problems are highly sought after. Chapter 5 establishes a novel mechanochemical method for synthesizing acenes with Sn(II) salts. Spacers, in the context of this thesis, refer to the group(s) used to link chromophores into oligomers with precise control over the relative coupling and orientation of the chromophores. The spacer is the lynchpin of all oligomeric models and the synthesis of novel spacers based on diamantane and biphenyl is described in Chapter 6. In Chapter 7, the design and synthesis of verdazyl-radical dimers is described. Though seemingly unrelated, the radical dimers' efficiency as a dynamic nuclear polarization agent is largely dictated by various coupling factors mediated by the spacer akin to singlet fission model compounds.
Additional file 1 of Occupational stress and burnout experience among healthcare workers compounded by the COVID-19 pandemic in Africa: a scoping review protocol
Creators: name: Agyemang, George
name: Bema, Yaa
name: Eturu, Deborah Aba
name: Bawontuo, Vitalis
name: Kuupiel, Desmond
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Sociology
subject: FOS: Sociology
subject: Information Systems not elsewhere classified
subject: Cancer
subject: Science Policy

description: Additional file 1: Supplementary file 1. PRISMA-P 2015 Checklist.
A Functional Neuroanatomical Investigation of the Biphasic Effects of Serotonin Reuptake Inhibition on Socio-Emotional Behaviour
Creators: name: Payet, Jennyfer
Publisher: name: La Trobe
Year: 2025
Subjects: classificationCode: 52
subject: Psychology
subject: FOS: Psychology
classificationCode: 520505
subject: Social psychology
classificationCode: 520301
subject: Clinical neuropsychology

description: A thesis submitted in total fulfilment of the requirements for the degree of Doctor of Philosophy to the School of Psychology and Public Health, La Trobe University, Victoria, Australia.
Additional file 1 of The use of ‘poisonous insecticidal solutions’ in bookbinding: coping with historic pesticide treatments in the archive
Creators: name: Angelova, Lora V.
name: Nawaz, Sadat
name: Kafadaroğlu, Barbara
name: Paz, Boaz
name: Moreta, Francisco
name: Woollaston, Helen
name: Vermeulen, Marc
name: Vervoorst, Juergen
Publisher: name: figshare
Year: 2024
Subjects: subject: Biochemistry
subject: Physiology
subject: FOS: Biological sciences
subject: Biotechnology
subject: Environmental Sciences not elsewhere classified
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: Developmental Biology
subject: Cancer
subject: Plant Biology

description: Additional file 1: Figure S1. The Crown Agents for the Colonies, Standard Specification No. 40—Binding of Book. CAOG 12/88/6. Figure S2. Right paste-down of FCO 141/14515 with handwritten note deriding quality of paper in ledgers issued by the Government Printer. Figure S3. Calibration line for DDT-pp; range from 0.002 µg/ml to 0.05 µg/ml. Figure S4. Chromatogram for DDT-pp; 0.05 µg/ml calibration solution, Positive control at 0.02 µg/ml, negative control and a positive sample, respectively. Tables S1. The GC-MS/MS results from FERA Science Ltd are presented in the three tables below followed by the results from ALAB GmBH. Table S2. Limit of detection for each sampling method used for occupational health testing by Synergy Environmental Solutions Ltd. Table S2. Summary of health and safety approaches taken by other organisations with regard to insecticide-treated collections. Sources are included in main manuscript reference list.
Additional file 1 of Utility of hand-held ultrasound for image acquisition and interpretation by trained Kenyan providers
Creators: name: Wanjiku, Grace
name: Dreizler, Lindsay
name: Wu, Shirley
name: Baird, Janette
name: Wachira, Benjamin
Publisher: name: figshare
Year: 2024
Subjects: subject: Artificial Intelligence and Image Processing
subject: FOS: Computer and information sciences

description: Additional file 1. Appendix A: Observed Structured Clinical Exam Assessment (OSCE) Form.
Additional file 1 of Patient engagement in a national research network: barriers, facilitators, and impacts
Creators: name: Gonzalez, Miriam
name: Ogourtsova, Tatiana
name: Zerbo, Alix
name: Lalonde, Corinne
name: Spurway, Amy
name: Gavin, Frank
name: Shikako, Keiko
name: Weiss, Jonathan A.
name: Majnemer, Annette
Publisher: name: figshare
Year: 2024
Subjects: subject: Cancer
subject: Science Policy
subject: Mental Health
subject: Infectious Diseases
subject: FOS: Health sciences

description: Additional file 1. “Stakeholder Engagement in CHILD-BRIGHT Network” contains the October 2021 Engagement evaluation report with preliminary findings produced by CHILD-BRIGHT.
A kinaesthetic approach to analyzing early recordings
Creators: name: Ho, Jocelyn
Publisher: name: Zenodo
Year: 2025
Subjects: subject: Music
subject: Humanities
subject: FOS: Humanities

description: Abstract 

This paper proposes a kinaesthetic methodology for the analysis of early recordings, focusing on the bodily engagement of performers as a means of bridging the experiential gap between contemporary analysts and historical performers. Traditional approaches to early recording analysis often rely on either empirical analysis or comparative analysis with historical texts, leaving a disconnect between the analyst and the embodied act of performance. Through a four-stage process—emulation, articulation of embodiment, metaphor construction, and performer-informed interpretation—this study demonstrates how a bodily-informed, phenomenological approach can yield deeper insights into historical performance practice. Using the 1905 recording of pianist Yolanda Mero and a 1904 recording of Alessandro Moreschi as two case studies, the proposed method facilitates an affective and embodied engagement with historical recordings.
Additional file 1 of Distinct gene signatures of monocytes and B cells in patients with giant cell arteritis: a longitudinal transcriptome analysis
Creators: name: Matsumoto, Kotaro
name: Suzuki, Katsuya
name: Yoshida, Hiroto
name: Magi, Mayu
name: Matsumoto, Yoshihiro
name: Noguchi-Sasaki, Mariko
name: Yoshimoto, Keiko
name: Takeuchi, Tsutomu
name: Kaneko, Yuko
Publisher: name: figshare
Year: 2024
Subjects: subject: Genetics
subject: FOS: Biological sciences

description: Additional file 1: Supplementary Table 1. Treatment regimens at sample acquisition in GCA.
Grasping at Illusions: Stimulating the skin to investigate the cutaneous contribution to kinesthesia in the human hand
Creators: Leverett, Jessica A.
Publisher: name: University of Alberta Library
Year: 2024

How do you know your hand is moving without looking at it? This “movement sense”, known as kinesthesia, is thought to be primarily informed by receptors in muscles; specifically, the muscle spindles. As we move, muscle spindles discharge when muscles lengthen and, thus, most research has focussed on these receptors. However, receptors in the skin, known as cutaneous receptors, also discharge when we move, although their role in kinesthesia remains less clear. This thesis project was designed to investigate the role of cutaneous receptors in kinesthesia by using electrical stimulation to create illusory hand movements; namely, flexion of the index finger. The frequency and intensity of the stimulation were modulated, separately and simultaneously, to test hypotheses regarding how the central nervous system may use frequency coding and population coding from cutaneous receptors in kinesthesia. Thirty-four healthy participants (22 females, 12 males; aged 18-33) with no neurological or musculoskeletal impairments that affect the movement or sensation in the hands were recruited. Stimulation was applied to the superficial branch of the radial nerve on the dorsum of the right hand. A total of 10 trials were delivered in a random order. Each trial consisted of one of four stimulation patterns: 1) frequency-modulated: the frequency of the stimulation was sinusoidally increased and decreased with intensity held at perceptual threshold; 2) intensity-modulated: stimulation intensity was sinusoidally increased and decreased with frequency held at 55 Hz; 3) combined-modulation: stimulation frequency and intensity were synchronously increased and decreased; 4) sham: stimulation frequency was held at 55 Hz and intensity was held at perceptual threshold throughout the trial. If participants perceived movement, they were instructed to keep their right (stimulated) hand relaxed and to mimic the movements with their left (unstimulated) hand. Prevalence of illusory movements and movement amplitudes of the index finger were quantified using passive motion capture cameras. In total 25/34 (74%) of participants perceived illusory movements, with the mean amplitude of movement ranging from 1-25. There were no significant differences between the amplitudes of the frequency-modulated, intensity-modulated, or combined-modulation trials. However, the amplitudes of all three modulated trials were significantly larger than the sham trial. These results suggest that cutaneous receptors play a role in kinesthesia, and that the CNS uses frequency coding and population coding from cutaneous receptors to inform kinesthesia. The simple method used in this project may have applications for the investigation of other basic research questions, or integration with other sensory stimuli such as virtual reality.
Chemical Partitioning of Emerging Indoor Organic Pollutants
Creators: Wu, Shuang
Publisher: name: University of Alberta Library
Year: 2024

Given that modern humans spend approximately 90% of their time indoors, the indoor environment is crucial for chemical exposure. Emerging indoor organic pollutants, such as microbial volatile organic compounds (MVOCs) from molds, impact air quality and health. Additionally, flavoring agents released from vaping can lead to thirdhand exposure. The phase distribution of these pollutants affects how humans are exposed. However, predicting the environmental behavior of indoor volatile organic compounds (VOCs) remains challenging due to insufficient data.

This study uses a chemical two-dimensional (2D) partitioning model to visualize pollutant phase distributions in a triphasic indoor system (air, polar, and weakly-polar reservoirs). It highlights the importance of accurately measuring partitioning coefficients, especially Henry’s Law constant (H), which is affected by hydration and liquid-phase reactions. Combining experimental methods like inert gas-stripping (IGS) method and variable phase ratio headspace (VPR-HS) with the model techniques, the study reveals that VOCs often distribute between indoor air and weakly polar reservoirs, with distribution dependent on environmental conditions. Additionally, chemical partitioning can result in persistent indoor exposure.
Additional file 3 of Patient engagement in a national research network: barriers, facilitators, and impacts
Creators: name: Gonzalez, Miriam
name: Ogourtsova, Tatiana
name: Zerbo, Alix
name: Lalonde, Corinne
name: Spurway, Amy
name: Gavin, Frank
name: Shikako, Keiko
name: Weiss, Jonathan A.
name: Majnemer, Annette
Publisher: name: figshare
Year: 2024
Subjects: subject: Cancer
subject: Science Policy
subject: Mental Health
subject: Infectious Diseases
subject: FOS: Health sciences

description: Additional file 3. “GRIPP2 PPI reporting Checklist” contains the results of the GRIPP2 short form reporting checklist.
Work-In-Progress: Integrating Critical Pedagogy with Project- Based Learning
Creators: name: Lundell, Ryan
name: Montoya, Jonathan
name: Nava, Pedro
Publisher: name: Stanford Digital Repository
Year: 2024
Subjects: subject: Neoliberalism
subject: Neoliberal Logic
subject: Interdisciplinary Curriculum
subject: Critical pedagogy
subject: Critical Consciousness

description: The separation of disciplines in secondary education is an inherent obstacle to project-based learning (PBL): educators go years without meaningful collaboration, critical feedback, or self-reflection (Jacobs, 2010). As a result, many inhabit an isolated bubble where no space is given to interdisciplinary collaboration; this isolation limits the authenticity of the projects students can produce. Compounding the dilemma is neoliberal logic, which disseminates the model of the market to all domains and activities (Brown, 2017). The curriculum is depoliticized; students are motivated to excel academically so they can compete in the market rather than work towards more societal equity. Additionally, marginalized groups are tracked into vocational pathways that focus only on basic skills training and give no space to critical thinking, which hurts the worker’s ability to confront and transform inequitable neoliberal policies (Darder, 2017). While PBL in STEM and vocational pathways have positive impacts on teaching and learning outcomes, implemented without a critical pedagogy framework, PBL has not been shown to increase critical consciousness (Montoya et al., 2018). This research aims to discover how PBL and an interdisciplinary curriculum (Montoya et al., 2020) implemented through a framework of critical pedagogy can impact the critical consciousness of students and teachers.
Supplementary document for Machine-learning iterative optimization for all polarization-maintaining linear cavity Er:fiber laser - 6573681.pdf
Creators: name: Zhao, Minghe
name: Liu, Xuanyi
name: Fu, Hongyan
name: Li, Qian
Publisher: name: Optica Publishing Group
Year: 2025
Subjects: Uncategorized

description: The supplementary material showing: the relationship between the modulation depth and the orientation of half-wave plate in the cavity; Optimization effects of the algorithm for different values of a1 and a2 in the cost function.
title: Growing Intergenerational Resilience for Indigenous Food Sovereignty through Home Gardening
Creators: name: Budowle, Rachael
name: Arthur, Melvin
name: Porter, Christine
Publisher: name: University of Wyoming
Year: 2020
Subjects: subject: research brief
subject: Intergenerational Resilience
subject: Food Sovereignty
subject: Community Resilience
subject: Social-Ecological Systems
subject: Sovereign Storytelling
subject: Growing Resilience
subject: Indigenous
subject: Historical Trauma

description: Growing Resilience, a community-based participatory research project designed to promote health and wellbeing, provided installation and maintenance support for home food gardens to 96 primarily Eastern Shoshone and Northern Arapaho families living in the Wind River Reservation in Wyoming. The authors of this study investigated how participants' stories about gardens are rooted in family relationships, knowledge, and practices through time. In present, past, future, and cross-generational frames, families transmitted resilience through gardening across generations.
Influence of Airborne Ocularly Assessed Stand Density on Wildfire Escapes and Containment Challenges
Creators: Stack, Andrew P
Publisher: name: University of Alberta Library
Year: 2024

The relationship between increasing forest density and increasing wildfire behaviour is well established.

However, in the unique case of initial attack wildfire operations, traditional data collection methods to

measure forest density are both impractical and unsafe. As an alternative approach, aerial ocular

assessment to rapidly determine forest density and its impacts on wildfire behaviour is examined.

Wildfire assessment photography, taken during the initial action phase of wildfire suppression in pure

boreal spruce (Picea mariana) forests, served as an excellent opportunistic dataset to develop a series of

ordinal wildfire behaviour and forest density metrics for testing. This approach explored two scenarios:

1) the effects of forest density on containment escapes, where suppression efforts failed to contain a

wildfire to less than 2 hectares before being classified as “being held,” and 2) the effects of forest

density on wildfire containment challenges, where crews arrived at an incident where the wildfire had

already exceeded 0.5 hectares before suppression began. The ocularly assessed metrics were merged

with recorded weather and Fire Weather Index System values from nearby fire weather stations

(altogether totaling 33 variables) and analyzed using logistic regression in the form of bi-directional

generalized linear models. A parallel study, using LiDAR classification of forest structure in lieu of forest

density assessed in wildfire photographs, was also performed.

Results of this study indicate that in three of the four tested scenarios (ocularly assessed density and fire

escapes, LiDAR classified forest structure and fire escapes, and LiDAR classified forest structure and

containment challenges), forest structure and increasing density were found to be significant with

respect to an increase in wildfire behaviour. Further results also highlight the significance of wildfire

smoke column attributes in scenario outcomes. The selected models all performed well with C-statistics

0.75, however lacked predictive capability.
An evidential time-to-event prediction model based on Gaussian random fuzzy numbers
Creators: name: Huang, Ling
name: Xing, Yucheng
name: Denoeux, Thierry
name: Feng, Mengling
Publisher: name: arXiv
Year: 2024
Subjects: subject: Machine Learning (cs.LG)
subject: FOS: Computer and information sciences

description: We introduce an evidential model for time-to-event prediction with censored data. In this model, uncertainty on event time is quantified by Gaussian random fuzzy numbers, a newly introduced family of random fuzzy subsets of the real line with associated belief functions, generalizing both Gaussian random variables and Gaussian possibility distributions. Our approach makes minimal assumptions about the underlying time-to-event distribution. The model is fit by minimizing a generalized negative log-likelihood function that accounts for both normal and censored data. Comparative experiments on two real-world datasets demonstrate the very good performance of our model as compared to the state-of-the-art.
title: Resisting extractivism in the Sperrins: Countermapping and making relatives for post-extractive futures
Creators: name: Cirefice, V'cenza
Publisher: name: University of Galway
Year: 2025
Subjects: subject: Extractivism
subject: Resistance
subject: Feminist Political Ecology
subject: Northern Ireland
subject: Decolonial
subject: Participatory Research
subject: Arts, Social Sciences & Celtic Studies
subject: Geography

description: As the extractive frontier expands out into peripheralised parts of Europe, branded as critical for the green transition, communities are resisting. Research on resistance to this extractivism has ballooned in recent years. I build on feminist and decolonial scholarship which has conceptually widened extractivism from a physical removal of resources to be understood as a logic, worldview, a way of relating in the world rooted in coloniality and about relations of power. This thesis contributes to these debates around grassroots resistance to extractivism by asking in what ways are communities resisting extractive logics in the Sperrin Mountains? Here in County Tyrone, North of Ireland communities have been resisting gold mining for almost ten years. There remains a large research gap in how communities resist extractivism in Ireland and particularly the North of Ireland. I use a mix of activist, creative and participatory methodologies to challenge academic extractivism. I detail how extractive logics are manifesting in the Sperrins as colonial constructions of place, embodied and emotional slow violence and through social engineering. I argue that in challenging these extractive logics, the resistance movement is “Making Relatives” and transforming relations with the human and more-than-human world. This is characterised in three ways, firstly through building relations within the campaign based on care and commoning, secondly, through relations with the more-than-human and thirdly by building relations with other translocal frontline communities. This is significant for understanding ways to challenge oppressive systems that underlie extractivism and work towards post-extractive futures. I contribute to debates around the ontological question of extractivism and how we build alternative life-worlds.
Additional file 1 of TaME-seq2: tagmentation-assisted multiplex PCR enrichment sequencing for viral genomic profiling
Creators: name: Hesselberg Løvestad, Alexander
name: Stosic, Milan S.
name: Costanzi, Jean-Marc
name: Christiansen, Irene Kraus
name: Aamot, Hege Vangstein
name: Ambur, Ole Herman
name: Rounge, Trine B.
Publisher: name: figshare
Year: 2024
Subjects: subject: Genetics
subject: FOS: Biological sciences

description: Additional file 1. Table S1: Overview of cytology-based and/or histology classification of clinical samples positive for HPV51, 52, and 59. (LSIL – low-grade intraepithelial lesion, ASC-US - atypical squamous cells of undetermined significance, HSIL- high-grade intraepithelial lesion, ASC-H - Atypical squamous cells- cannot exclude high-grade squamous intraepithelial lesion, CIN1- Cervical intraepithelial neoplasia grade 1, CIN2-Cervical intraepithelial neoplasia grade 2, ND-not determined, NR-not relevant). Table S2: Read counts and sequencing statistics of HPV positive cervical cell samples, HPV harboring plasmids (Equalis proficiency panel) and SARS-CoV-2 positive clinical samples (NA-not applicable). Table S3: Location of the integration breakpoints found in duplicates of 10-HPV59 sample and number of unique discordant read pairs and junction reads at the integration breakpoints. Table S4: Number of minor nucleotide variants (MNVs) grouped by HPV gene, identified in run 1 and run 2. Figure S1: HPV genome sequencing coverage in replicates 10a-HPV59 and 10b-HPV59. Figure S2. IGV visualization of HISAT2 and LAST alignments of 10a-HPV59 and 10b-HPV59 to human genome indicating HPV-human integration breakpoints. (a) Integration breakpoint between chromosome 2 (GRCh38/hg38) and HPV59 E2. (b) Integration breakpoint between chromosome 2 and HPV59 E5. HISAT2 reads are presented with dark gray color. Parts of the LAST reads mapping to human are light gray, while parts mapping to HPV59 are multi-colored. Red arrows point to the exact breakpoint positions. Figure S3. Number of minor nucleotide variants presented as violin plots across the different virus types, A) HPV52, HPV52, and HPV59; B) SARS-CoV-2. Violin plot shows the probable density of the data, using kernel density estimation. Box-and-whisker plots are added to show the median number (horizontal line), 25% and 75% percentiles (box), minimum and maximum values (whiskers). Figure S4: Mutational signatures found between technical replicates in the two sequencing runs. The mean proportion of 96 trinucleotide substitution types is shown below the plots across the different diagnostic categories. Error bars represent the standard error of the mean.
Dimensionality affects memory for events in immersive virtual reality
Creators: Feminella Duarte Da Costa, Julia
Publisher: name: University of Alberta Library
Year: 2024

Real-world events are three-dimensional (3D) and immersive. Prior research using virtual reality (VR) has suggested that 3D experiences may boost memory performance but are limited because they do not directly compare 3D and 2D experiences in fully immersive settings. Across two studies, we investigated how dimensionality affects memory for real-world events experienced in an immersive virtual reality (VR). Participants experienced 360-degree videos of real-world events (e.g., standing in a train station) while wearing an immersive VR headset. We manipulated the dimensionality of experiences by presenting the videos in monoscopic (2D) and stereoscopic (3D) VR. The events also varied in emotional valence (negative, neutral, positive). Memory for the VR events was tested by presenting a partial screenshot from the video and asking participants to provide subjective ratings about the emotional intensity, vividness, quality of scene-related aspects, and visual perspective associated with their memories. In the first experiment, we tested memory immediately after the encoding of the events. In the second, encoding took place in two sessions separated by 24 hours, with memory being tested immediately after the second session. The results revealed that 3D events were experienced with a stronger sense of presence, which was associated with greater vividness and scene-related qualities of memory during remembering. This effect of dimensionality was consistent when memory was tested after a retention interval. We further provide the average emotionality ratings that can be used to base future studies using immersive VR. Our findings indicate the potential of VR and 360° videos to investigate key properties of real-world memories such as their 3D nature.
Generalized Entropy and Solution Information for Measuring Puzzle Difficulty
Creators: Shen, Junwen
Publisher: name: University of Alberta Library
Year: 2024

Metrics for problem difficulty are used by many puzzle generation algorithms, as well as by adaptive algorithms that are expected to provide players with the puzzles at the correct level of difficulty. A recently proposed general metric, puzzle entropy, combines an analysis of game mechanics with a model of player knowledge in the form of inference rules to predict problem difficulty. The entropy of a puzzle is the amount of information required, given a player’s knowledge about the puzzle, to describe a solution to a puzzle. This thesis generalizes the concepts of puzzle entropy and solution information, providing a better foundation for the previous work and creating new algorithms, Minimum Solution Information and Total Solution Information. While functionally similar to past work, the new algorithms allow knowledge about a puzzle to be represented as a policy. We then evaluate the impact of inference rules, policies, and player knowledge in the 2016 game The Witness.
Supplementary document for Design, Optimization and Characterization of Deep Sub-Wavelength Evanescent Orders in Terahertz Metagratings - 6573953.pdf
Creators: name: Rane, Shreeya
name: Punjal, Ajinkya
name: Prabhu, Shriganesh
name: Chowdhury, Dibakar Roy
Publisher: name: Optica Publishing Group
Year: 2025
Subjects: Uncategorized
Photonics, Optoelectronics and Optical Communications

description: Supplemental document
Additional file 1 of Interventions to improve the implementation of evidence-based healthcare in prisons: a scoping review
Creators: name: Blackaby, Jenna
name: Byrne, Jordan
name: Bellass, Sue
name: Canvin, Krysia
name: Foy, Robbie
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Biotechnology
subject: Sociology
subject: FOS: Sociology
subject: Cancer
subject: Science Policy

description: Additional file 1. Scoping Reviews (PRISMA-ScR) Checklist. This file contains details of the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) Checklist (Tricco et al., 2018).
Adolescent Education for Prevention of Sexually Transmitted Diseases
Creators: name: Mullins, Aubrey
Publisher: name: Oregon Health and Science University
Year: 2024
Subjects: subject: 19
subject: Oregon
subject: STI prevention
subject: STD prevention
subject: Adolescent STD education
subject: STD prevention education

description: With increasing rates of sexually transmitted diseases, this community outreach project investigated efficacious strategies to decrease STI rates, including adolescent education. An evidence-based education presentation was created and provided to a group of high school students. The project includes a research paper on adolescent education for prevention of STI transmission, an educational presentation, a summary paper, and a community outreach poster.
Optimizing Mpro expression and advancing pan-coronavirus inhibitors: a strategic approach for pandemic preparedness
Creators: Maplethorpe, Cassidy GA
Publisher: name: University of Alberta Library
Year: 2024

With the ever-evolving landscape of viral threats, the need for versatile and broadly effective antiviral treatments has become paramount. In the last 20 years, coronaviruses have been responsible for two epidemics and a pandemic. The coronavirus main protease, Mpro, has emerged as a key drug target, exemplified by the COVID-19 therapy Paxlovid™, which includes the Mpro inhibitor nirmatrelvir. Mpro's central role in viral replication, along with its high conservation in sequence, structure, active site, and substrate preference across all coronaviruses, makes it an attractive target for therapeutic intervention. This study aims to evaluate the efficacy of Mpro inhibitors on human coronaviruses and identify pan coronavirus inhibitors. We cloned, expressed, and purified the Mpro’s of five human coronaviruses, SARS-CoV-2, CoV-OC43, -229E, -NL63, and -MERS, and determined the enzymatic activities for each. While Mpro from SARS-CoV-2 and MERS behaved well after purification, Mpro from 229E, NL63, OC43 and HKU1 required optimisation to increase yield and stability for subsequent assays. Once optimal protein with high purty was obtained, we then assessed the inhibition profiles and determined IC50 values for several inhibitor scaffolds and harnessed several attempts at crystalizing the drug-inhibitor complexes of the best performing inhibitors. Our findings demonstrate the broad-spectrum capabilities of both nirmatrelvir and our novel Mpro inhibitors by testing their potency against many coronaviruses. In our analysis, several SARS-CoV-2 Mpro inhibitors showed pan coronavirus inhibition in the nanomolar range. Notably, inhibitors AVI-8122, -8106, and -8053, showed IC50s ranging from 4.5- 60 nM. These compounds, along with several inhibitors in various stages of clinical development, such as simnotrelvir, ibuzetrelvir, nirmatrelvir, MK-7845 and ensitrelvir, indicate the potential for broad-spectrum capabilities of coronavirus therapeutics targeting Mpro. Our inhibitors have the most potent pan-coronaviral inhibiting abilities with lower IC50 values and higher efficacy cross all human-infecting coronavirus genera. Further preclinical and toxicological evaluation is ongoing to fully assess these compounds' therapeutic potential. Given the ongoing global concern regarding coronaviruses and the potential for future outbreaks, our findings underscore the importance of developing robust antiviral strategies. These Mpro inhibitors hold promise not only for current pandemic response, but also for enhancing preparedness for future coronavirus threats.
المستودعات الرقمية المؤسسية في الجامعات السعودية: نحو رؤية لمشروع وطني لدعم مبادرات إنشائها وإدارتها
Creators: name: الضويحي، فهد
Publisher: name: Zenodo
Year: 2014
Subjects: المستودعات المؤسسية
السعودية
institutional repositories
Saudi Arabia
Open Access

description: تتناول الدراسة المستودعات المؤسسية في الجامعات السعودية
Additional file 2 of Factors related to the mortality risk of severe hand, foot, and mouth diseases (HFMD): a 5-year hospital-based survey in Guangxi, Southern China
Creators: name: Peng, Yuanjun
name: He, Weitao
name: Zheng, Zhigang
name: Pan, Peijiang
name: Ju, Yu
name: Lu, Zhenwei
name: Liao, Yanyan
name: Wang, Hailong
name: Zhang, Chao
name: Wang, Jing
name: Jiang, Lina
name: Liang, Hao
name: Chen, Minmei
name: Ye, Li
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Neuroscience
subject: Immunology
subject: FOS: Clinical medicine
subject: Mathematical Sciences not elsewhere classified
subject: Cancer
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Virology
subject: Computational Biology

description: Additional file 2. Case Questionnaire for severe or fatal Hand, foot, and mouth Disease.
AUT885215_Supplemental_table_2 – Supplemental material for The effect of motor and physical activity intervention on motor outcomes of children with autism spectrum disorder: A systematic review
Creators: name: Ruggeri, Anneliese
name: Dancel, Alina
name: Johnson, Robert
name: Sargent, Barbara
Publisher: name: SAGE Journals
Year: 2019
Subjects: subject: 170199 Psychology not elsewhere classified
subject: FOS: Psychology
subject: 110319 Psychiatry (incl. Psychotherapy)
subject: FOS: Clinical medicine
subject: 111799 Public Health and Health Services not elsewhere classified
subject: FOS: Health sciences
subject: Education
subject: 130312 Special Education and Disability
subject: FOS: Educational sciences

description: Supplemental material, AUT885215_Supplemental_table_2 for The effect of motor and physical activity intervention on motor outcomes of children with autism spectrum disorder: A systematic review by Anneliese Ruggeri, Alina Dancel, Robert Johnson and Barbara Sargent in Autism
Supplementary document for Wavelength scaling of high harmonic yield and cutoff energy in solids driven by mid-infrared pulses - 6574031.pdf
Creators: name: Wan, Zhongjun
name: Guan, Zhong
name: Liu, Jiaqi
name: Yang, Hang
name: Tian, Kan
name: He, Linzhen
name: Xiang, Maoxing
name: Hu, Bo
name: wang, weizhe
name: Yang, Xuemei
Publisher: name: Optica Publishing Group
Year: 2025
Subjects: Uncategorized

description: Supplementary Information
title: Structure-based function assignment for archaellum regulatory network proteins of Sulfolobus acidocaldarius
title: Struktur-basierte Funktions Annotierungen Archaellum regulierender Netzwerk Proteine aus Sulfolobus acidocaldarius
titleType: AlternativeTitle
Creators: name: Korf, Lukas
Publisher: name: Philipps-Universität Marburg
Year: 2024
Subjects: ArnB
Endosymbiose
GPN-loop GTPases
structure to function assignment
endosymbiosis
Strukturbiologie
Structural biology
Protein biochemistry
Proteinbiochemie
Life sciences
Biowissenschaften, Biologie
ArnA
Funktions Annotierung
Archaellum Regulatory Network
Sulfolobus acidocaldarius
Struktur zu Funktions Analyse

description: Der Ursprung des Lebens ist ein komplexes Thema, das weltweit wissenschaftliche Diskussionen befeuert. Im Allgemeinen ist es akzeptiert, dass die Endosymbiose eine zentrale Rolle bei der Eukaryogenese spielt. Für diese Arbeit wurde die inside-out
Hypothese, welche sich auf die Asgard Archaeen stützt und ein frühes Endomembransystem voraussetzt, als Ausgangslage angenommen. Da Asgard
Archaeen noch nicht kultiviert wurden, stellen die TACK Archaeen mit ihrem archaealen Modellorganismus Sulfolobus acidocaldarius einen der nächsten, verfügbaren Verwandten der Eukaryoten dar. Interessanterweise wurde das Protein ArnB des archaellum regulatory networks (Arn), welches strukturelle Homologien mit dem Sec23/24 Protein des COPII-systems aufweist, in S. acidocaldarius Exosomen gefunden. Entsprechend erweist sich ArnB als vielversprechendes Protein, um den prä-endosymbiotischen Transport in Archaeen zu untersuchen. Diese Arbeit umfasst eine Zusammenfassung der Forschungsergebnisse zu ArnB und seinen assoziierten Proteinen. Hervorzuheben ist dabei die Untersuchung der Interaktion von ArnB mit dem ZnF- und FHA-Domänen enthaltenden ArnA, welche eine sequenzielle, phosphorylierungsabhängige strukturelle Transition mit globalen Änderungen offenbart. Die Untersuchung des ArnAB-Komplexes ergab zum einen neuartigen sequenziellen Phosphorylierungsmechanismus und zeigte die Gegenwart des Sec23/24-Kernmotivs in allen Domänen des Lebens auf. In Zuge dieser Analysen, konnten dem ArnAB-Komplex bzw. dem Sec23/24-Kernmotiv zahlreiche potenzielle
Funktionen zugeschrieben werden, die von der Beteiligung am Ubiquitin-Netzwerk bis
zur Bildung von COPII-Vesikeln und Assemblierungsrollen reichen. Zudem beinhaltet
diese Arbeit die Charakterisierung einer GPN-Loop-GTPase aus S. acidocaldarius,
SaGPN, welche ebenfalls mit dem Arn-System assoziiert ist und potentiell mit dem ArnAB-Komplex interagiert. GPN-Loop-GTPasen stellen einen eigenen interessanten GTPase-Typ dar, der durch nukleotidunabhängige Homodimere gekennzeichnet ist.
Durch die Kristallstrukturen die in dieser Arbeit gelöst wurden, lassen sich mechanistische Unklarheiten im Zusammenhang mit GPN-Loop-GTPasen lösen und ein Lock-Switch-Rock-Mechanismus für archaeale GPN-Loop-GTPasen ableiten.
Zusammenfassend trägt diese Arbeit zum allgemeinen Verständnis der GPN-Loop-
GTPasen bei und hebt das ArnAB-Sec23/24-Kernmotiv als eine der häufigsten und funktional vielfältigsten Protein-Faltungen in allen Bereichen des Lebens hervor.
title: Dosimetry of high energy photon beams in the presence of magnetic fields
Creators: name: Alissa, Mohamad
Publisher: name: Philipps-Universität Marburg
Year: 2024
Subjects: Medical sciences Medicine
Medizin

description: Die moderne Strahlentherapie zielt darauf ab, gesundes Gewebe und Risikoorgane (OARs) optimal zu erhalten, während gleichzeitig eine hohe Dosis im Zielvolumen (PTV) appliziert wird. Um dieses Ziel zu erreichen, wurden mehrere Geräte entwickelt, darunter die Integration eines klinischen Elektronen-Linear-Beschleunigers mit einem Magnetresonanztomographie-Gerät (MRT). Diese Integration ermöglicht eine Echtzeitbildgebung der Anatomie des Patienten während der Strahlenbehandlung, was eine verbesserte Zielgenauigkeit und adaptive Behandlungsstrategien ermöglicht. Bilder können für die Lokalisierung des Tumors und die Positionierung des Patienten vor der Behandlung sowie für Techniken zur Bewegungsminderung, einschließlich Zielverfolgung und Gating, verwendet werden. Zusätzlich können Veränderungen in der Anatomie, wie Gewichtsverlust oder Organfüllung, durch Anpassung des Behandlungsplans an die aktuellsten MRT-Bilder berücksichtigt werden.
Für eine präzise Dosisapplikation im MR-Linac ist es allerdings notwendig, die Wirkung der Lorentzkraft auf die Strahlung zu berücksichtigen. Aufgrund der Lorentzkraft kommt es zu einer Ablenkung der geladenen Sekundärteilchen und damit zu einem veränderten Ansprechen der eingesetzten Detektoren. Vor diesem Hintergrund bestand das Hauptziel der vorliegenden Arbeit darin, das Verhalten von Ionisationskammern in Magnetfeldern
zu untersuchen. Daher wurden die Strahlqualitätskorrekturfaktoren für hochenergetische Photonenstrahlen in Ionisationskammern (SNC600c, SNC125c) und hochenergetische Elektronenstrahlen in (SNC600c, SNC125c, SNC350p) gemäß Dosimetrieprotokollen wie TG-51, TRS 398 und DIN 6800-2 berechnet. Zusätzlich wurde mittels des Fano-Hohlraumtests bestätigt, dass die Monte-Carlo-Codes eine hohe Genauigkeit (0,1%) in Anwesenheit eines Magnetfeldes aufweisen. Schließlich wurde die Änderung des Ansprechvermögens der beiden Ionisationskammerm SunNuclear SNC600c und SNC125c in äußeren magnetischen Feldern, d.h. der Korrektionsfaktor kB,Q, experimentell und mittels Monte-Carlo Simulationen
ermittelt. Die Ergebnisse haben gezeigt, dass der Korrektionsfaktor kB,Q sowohl von der Stärke des äußeren Magnetfeldes als auch von der Größe des Messvolumens und der Orientierung der Kammerachse, und die Änderung des Ansprechvermögens beider Kammern ist minimal, wenn die Magnetfeldrichtung und die Kammerachse ausgerichtet und senkrecht zur Strahlrichtung sind. Darüber hinaus war in Anwesenheit eines Magnetfeldes bei der größeren Kammer SNC600c größer war als bei der kleineren Kammer SNC125c.
Zusammenfassend deckte diese Studie einen wichtigen Teil der Dosimetrie bei Vorhandensein von Magnetfeldern ab und bestätigte die Möglichkeit, die beiden untersuchten Ionisationskammern zur genauen Dosisbestimmung in Anwesenheit von Magnetfeldern nach Anwendung der Magnetfeldkorrektionfaktoren zu verwenden.
In vitro analysis of rhomboid protease PARL-mediated proteolysis towards understanding its role in mitochondrial health and Parkinson’s Disease
Creators: Brassard, Raelynn
Publisher: name: University of Alberta Library
Year: 2024

Rhomboid proteases are a superfamily of serine intramembrane proteases that are involved in an array of cellular processes, often playing a role in proteostasis. The mammalian mitochondrial rhomboid Presenilin-Associated Rhomboid Like (PARL) protease is a regulator of mitochondrial homeostasis. PARL plays a role in mitochondrial quality control and mitophagy through cleavage of PINK1, apoptosis through cleavage of PGAM5 (phosphoglycerate mutase family 5) and Smac/Diablo (second mitochondrial activator of caspases), and mitochondrial respiration through cleavage of TTC19 (Tetratricopeptide Repeat Domain 19). Interestingly, PARL has been associated with multiple human diseases including type-2 diabetes and Parkinson’s Disease, making it a protein of high interest to human health. However, while numerous substrates have been identified for PARL, much of how this protease is regulated at the molecular and cellular levels is unclear. The difficulty of generating pure and functional recombinant membrane protein, as well as a real-time quantitative assay to measure protease cleavage has impeded functional studies to address these concerns in the past.

Here, we successfully generate active recombinant human PARL, as its -cleaved form (a.a. 77-379)from two distinct eukaryotic protein expression systems; a yeast system using P. pastoris and the mammalian Expi293 ™ (Thermo Fisher Scientific) expression system. Using the recombinant intramembrane protease in detergent micelles or reconstituted into proteoliposomes, we are able to measure in vitro kinetics of proteolytic activity against internally quenched peptide substrates containing the transmembrane residues of known PARL substrates.

In Chapter 3, we are able to use this system to assess substrate cleavage preferences and the effect of lipid composition and distribution on enzyme activity. Additionally, we are able to utilize the kinetic assay to screen potential inhibitors against PARL designed based of the substrate specificity previously determined by our lab. Finally, Chapter 4 of this thesis examines the influence of Parkinson’s Disease associated mutations in the transmembrane region of PINK1, proximal to the cleavage site by PARL. Here, a mixture of cellular and in vitro assays are employed  to determine the effect of these mutations on proteolysis by PARL and uncover  the potential etiology of these mutations in disease pathogenesis.
The Effect of Visual Stimuli on Postural Control in Standing Humans
Creators: Hemakumara, Sisuri G
Publisher: name: University of Alberta Library
Year: 2024

Balance is crucial for upright stance and mobility, with the control of balance being fundamental to most daily activities including rising from bed and walking. Upright bipedal stance is inherently unstable as the majority of the body’s mass is elevated high above the feet, creating instability and giving rise to a natural sway pattern. Balance control is a complex process involving sensorimotor systems, including the visual, vestibular, and somatosensory systems. Specifically, visual feedback has been demonstrated to be a potent sensory cue as the absence of vision leads to increased postural sway, while motion of the visual surround induces compensatory sway. We hypothesized that a rapid, transient, visual stimulus, akin to the motion parallax relayed during a push or slip, would evoke a rapid balance corrective reaction in the direction of the visual stimulus. Moreover, we hypothesized that the reaction would habituate with repeated exposure and provision of a stable light touch reference would stabilize or mitigate the balance reactions. Forty participants received 10 small, transient, visual displacements using the “moving room” paradigm. Visual stimuli were 2.5 cm in amplitude with a peak velocity of 124 mm/s. Twenty participants received forward visual displacements and the other twenty participants received backward visual displacement. In addition, half the participants received a stable light touch reference. Following the first visual stimulus 39 out of the 40 participants leaned in the direction of the visual stimulus, as demonstrated by a shift in the position of the center of pressure. However, the latency of the response was slow and unlikely to be a rapid balance reaction. Upon repeated exposure to the stimulus participants continued to lean in the direction the stimulus. The participants that received a stable light touch reference during the visual displacements also demonstrated a lean in the direction of the visual stimulus, although with smaller amplitudes. These results suggest that the slow latency postural leans exhibited by the participants are not balance reactions, but rather likely represent a postural reorientation of the body to realign with the new position of the visual surround. We suggest that this postural reorientation serves to reposition the head within the relocated spatial frame of reference of the visual surround. Further, the continued repositioning behaviour with repeated exposure and with the provision of light touch suggests that vision is a potent sensory cue in maintaining postural orientation.
title: Photoannulierungen zu Dihydrophenalenonen und 1-Tetralonen
Creators: name: Schmalz, Veronika
Publisher: name: Philipps-Universität Marburg
Year: 2024
Subjects: Chemistry & allied sciences
Chemie

description: Die Reaktionsbedingungen der Photozyklisierung des Cyclopropylesters zum Dihydrophenalen wurden erfolgreich optimiert und ergaben das Produkt in einer Ausbeute von 93% unter Standardbedingungen. Eine sensitivity assessment wurde durchgeführt, um Erkenntnisse für eine verbesserte Reproduzierbarkeit zu gewinnen. 17 Derivate des Dihydrophenalens wurden mit Ausbeuten im Bereich von 50 – 99% über die entwickelte Methode dargestellt. Zudem konnte der Reaktionsmechanismus experimentell mittels Cyclovoltammetriemessungen, einem Deuterium Labeling-Experiment und weiteren Kontrollexperimenten untersucht und einem Photoredoxprozess zugeordnet werden. Die Bestimmung des KIEs zeigte, dass sich die Reaktion an einem deuterierten Substrat signifikant verlangsamt. Über die visual kinetic analysis (VKA) wurde ermittelt, dass die Reaktion durch die Bildung des Produktes beeinträchtigt wird (product inhibition). 
Die erstmalig Beobachtete Photohydroacylierung zum Tetralon wurde optimiert, mittels sensitivity assessment untersucht und erfolgreich auf 15 Substrate angewendet, die mit Ausbeuten im Bereich von 55 - 99% erhalten wurden. Der neuartige Reaktionsmechanismus wurde eingehend untersucht. Ein Deuterium-Labeling-Experiment wurde durchgeführt, das zu der Beobachtung eines selektiven 1,5 HAT-Prozesses führte. Zudem wurde der Reaktionsmechanismus mithilfe von Radikal Uhr Experimenten und Deuterium-scrambling-Experiment als eine Retro-Norrish-Typ-I Reaktion kategorisiert. Die Reaktion wurde außerdem mittels VKA untersucht und der postulierte Mechanismus wurde theoretisch über DFT-Berechnungen unterstützt. Somit wurde eine neue Photoreaktion erfunden, entwickelt und untersucht, die den anfangs gewählten Kriterien (Raumtemperatur, kurze Reaktionszeit, ohne Additive) entspricht.