Wickerhamomyces menglaensis Chai, Huang, Cheng, Liu & Hui, 2019, SP. NOV.
Creators: name: Chai, Chun-Yue
name: Huang, Lin-Na
name: Cheng, Han
name: Liu, Wen-Jing
name: Hui, Feng-Li
Publisher: name: Zenodo
Year: 2019
Subjects: Biodiversity
Taxonomy
Fungi
Ascomycota
Saccharomycetes
Saccharomycetales
Wickerhamomycetaceae
Wickerhamomyces
Wickerhamomyces menglaensis

description: DESCRIPTION OF WICKERHAMOMYCES MENGLAENSIS HUI & HUANG SP. NOV.   
 Wickerhamomyces menglaensis (meng.la.en′ sis. N.L. masc. adj. menglaensis of or belonging to the city of Mengla, Yunnan Province, PR China, the collection locality of the type strain of the species).   
 In YM broth after 3 days at 25 Ǫ C, the cells are ellipsoidal to elongate (3.0–6.7×5.9–8.2 µm) and occur singly or in pairs. Budding is multilateral (Fig. 2a). On YM agar after 3 days at 25 Ǫ C, the streak culture is butyrous, cream and convex with a smooth surface and has an entire margin. On Dalmau plates after 13 days on YCBS agar at 25 Ǫ C, pseudohyphae are formed (Fig. 2b), but true hyphae are not formed. Ascospores are not observed on YM, 5 % malt extract, cornmeal and YCBAS agar in pure and mixed cultures at 17 and 25 Ǫ C for up to 4 weeks. Glucose and raffinose (weak) are fermented. No fermentation of the following carbohydrates: galactose, sucrose, maltose, lactose, trehalose, D- xylose, cellobiose and melezitose. Assimilation of carbon compounds is as follows: D- glucose, D- xylose (weak), L- arabinose (weak), L- rhamnose (weak), cellobiose, salicin, arbutin, glycerol (weak), starch (weak), inulin (weak), D- glucitol, D- mannitol, D- glucono-1, 5-lactone, DL- lactate, succinate and citrate. Carbon sources not assimilated are as follows: D- galactose, L- sorbose, D- glucosamine, D- ribose, D- arabinose, sucrose, maltose, trehalose, melibiose, lactose, raffinose, melezitose, erythritol, ribitol, xylitol, L- arabinitol, galactitol, myo -inositol, 2-keto-D- gluconate, methanol, D- gluconate, D- glucuronate, D- galacturonate, 5-keto-D- gluconate and ethanol. Assimilation of nitrogen compounds are as follows: nitrate, nitrite, L- lysine (weak), glucosamine (weak) and D- tryptophan (weak). Cadaverine, creatine, creatinine, ethylamine and imidazole are not assimilated. Cell growth is observed in vitamin-free medium. Growth is observed at 35 Ǫ C. No growth on 50 % (w/v) glucose or 5 % (w/v) glucose/sodium chloride (10 %). Urea hydrolysis and diazonium blue B reactions are negative.   
The holotype, NYNU 1673, was isolated in August 2016, from rotting wood collected in Xishuangbanna Tropical Rainforest in Yunnan Province, PR China. It is preserved in a metabolically inactive state at Microbiology Lab, Nanyang Normal University, Henan, PR China. Ex-type cultures are deposited at the China Center of Industrial Culture Collection (CICC), Beijing, PR China, as strain CICC 31159, and at the Yeast Collection of the Westerdijk Fungal Biodiversity Institute (CBS), Utrecht, The Netherlands, as strain CBS 14689. The MycoBank number is MB 829372.
description: Published as part of Chai, Chun-Yue, Huang, Lin-Na, Cheng, Han, Liu, Wen-Jing & Hui, Feng-Li, 2019, Wickerhamomyces menglaensis f. a., sp. nov., a yeast species isolated from rotten wood, pp. 1509-1514 in International Journal of Systematic and Evolutionary Microbiology 69 (5) on pages 1512-1513, DOI: 10.1099/ijsem.0.003350, http://zenodo.org/record/6223927