title: ÐÐ¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐ¸ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Modeling the dynamics of stemcells
Creators: name: Ð¦ÑÐ¹ Ð¦Ð·ÑÐ²ÑÐ½Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: ÐÐ°ÑÐµÐ¼Ð°ÑÐ¸ÑÐµÑÐºÐ¾Ðµ Ð¼Ð¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
ÐÐ»ÐµÑÐºÐ° (Ð±Ð¸Ð¾Ð».)
Ð¿Ð»ÑÑÐ¸Ð¿Ð¾ÑÐµÐ½ÑÐ½ÑÐµ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°
ÑÐºÑÐ¿Ð¾Ð½ÐµÐ½ÑÐ¸Ð°Ð»ÑÐ½ÑÐ¹ ÑÐ¾ÑÑ ÐºÐ¾Ð»Ð¾Ð½Ð¸Ð¹
ÑÐ»Ð¸ÑÐ½Ð¸Ðµ ÐºÐ¾Ð»Ð¾Ð½Ð¸Ð¹
Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð´Ð¸ÑÑÑÐ·Ð¸Ð¸
human pluripotent stem cells
exponential colony growth
colony fusion
diffusion models

description: ÐÐ¾Ð½Ð¸Ð¼Ð°Ð½Ð¸Ðµ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð¾Ð², ÑÐ¿ÑÐ°Ð²Ð»ÑÑÑÐ¸Ñ ÑÐ¾ÑÑÐ¾Ð¼ ÐºÐ¾Ð»Ð¾Ð½Ð¸Ð¹ Ð¿Ð»ÑÑÐ¸Ð¿Ð¾ÑÐµÐ½ÑÐ½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°, ÑÐ²Ð»ÑÐµÑÑÑ Ð²Ð°Ð¶Ð½ÑÐ¼ Ð´Ð»Ñ ÑÐ¾Ð²ÐµÑÑÐµÐ½ÑÑÐ²Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÑÐ»Ð¾Ð²Ð¸Ð¹ Ð¸Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð°ÑÐ¸Ð¸ Ð¸ Ð¾ÑÐ±Ð¾ÑÐ° Ñ ÑÐµÐ»ÑÑ Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐµÐ³Ð¾ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ñ Ð² ÑÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸Ð²Ð½Ð¾Ð¹ Ð¼ÐµÐ´Ð¸ÑÐ¸Ð½Ðµ. Ð Ð°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð¾ÑÐ¾Ð±ÐµÐ½Ð½Ð¾ÑÑÐµÐ¹ ÑÐ¾ÑÑÐ° Ð¸ Ð¼Ð¸Ð³ÑÐ°ÑÐ¸Ð¸ ÐºÐ¾Ð»Ð¾Ð½Ð¸Ð¹ Ð¿Ð»ÑÑÐ¸Ð¿Ð¾ÑÐµÐ½ÑÐ½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð´Ð²ÑÑ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð»Ð¸Ð½Ð¸Ð¹ (CaSR Ð¸ H9). ÐÑÐ¾Ð²ÐµÐ´ÑÐ½ Ð°Ð½Ð°Ð»Ð¸Ð· ÑÐ°Ð·Ð¾Ð²Ð¾-ÐºÐ¾Ð½ÑÑÐ°ÑÑÐ½ÑÑ Ð¸Ð·Ð¾Ð±ÑÐ°Ð¶ÐµÐ½Ð¸Ð¹ ÑÐ°ÑÑÑÑÐ¸Ñ ÐºÐ¾Ð»Ð¾Ð½Ð¸Ð¹ Ð½Ð° Ð½ÐµÑÐºÐ¾Ð»ÑÐºÐ¸Ñ Ð²ÑÐµÐ¼ÐµÐ½Ð°Ñ, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð° Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐ° Ð¿Ð»Ð¾ÑÐ°Ð´ÐµÐ¹ ÐºÐ¾Ð»Ð¾Ð½Ð¸Ð¹ Ð¸ Ð¸Ñ ÑÑÐ°ÐµÐºÑÐ¾ÑÐ¸Ð¸. Ð¡ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¼Ð¾Ð´ÐµÐ»Ð¸ ÑÐºÑÐ¿Ð¾Ð½ÐµÐ½ÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÐ¾ÑÑÐ° ÐºÐ¾Ð»Ð¾Ð½Ð¸Ð¹ ÑÐ°ÑÑÑÐ¸ÑÐ°Ð½Ñ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÑ ÑÐ¾ÑÑÐ°. ÐÐ½Ð°Ð»Ð¸Ð· Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÐ¾Ð² Ð¿Ð¾Ð·Ð²Ð¾Ð»Ð¸Ð» Ð²ÑÐ´ÐµÐ»Ð¸ÑÑ ÑÐ°ÐºÑÐ¾Ñ, Ð²Ð»Ð¸ÑÑÑÐ¸Ð¹ Ð½Ð° Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ ÑÐ°Ð·Ð¼ÐµÑÐ¾Ð² ÐºÐ¾Ð»Ð¾Ð½Ð¸Ð¹ Ð¸ Ð¾ÑÐ»Ð¸ÑÐ½ÑÐ¹ Ð¾Ñ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð², ÑÐ²ÑÐ·Ð°Ð½Ð½ÑÑ Ñ Ð´ÐµÐ»ÐµÐ½Ð¸ÐµÐ¼ ÐºÐ»ÐµÑÐ¾Ðº Ð¸ ÑÐ»Ð¸ÑÐ½Ð¸ÐµÐ¼ ÐºÐ¾Ð»Ð¾Ð½Ð¸Ð¹. Ð­ÑÐ¾Ñ ÑÐ°ÐºÑÐ¾Ñ Ð¼Ð¾Ð¶Ð½Ð¾ Ð¸Ð½ÑÐµÑÐ¿ÑÐµÑÐ¸ÑÐ¾Ð²Ð°ÑÑ ÐºÐ°Ðº Ð¿ÑÐ¾ÑÐ²Ð»ÐµÐ½Ð¸Ðµ Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½ÑÑ Ð¿ÑÐ¾ÑÐµÑÑÐ¾Ð², ÑÐµÐ³ÑÐ»Ð¸ÑÑÑÑÐ¸Ñ Ð¿Ð»ÑÑÐ¸Ð¿Ð¾ÑÐµÐ½ÑÐ½ÑÐ¹ ÑÐµÐ½Ð¾ÑÐ¸Ð¿ ÐºÐ¾Ð»Ð¾Ð½Ð¸Ð¸. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐ¸ ÑÑÐµÐ´Ð½ÐµÐºÐ²Ð°Ð´ÑÐ°ÑÐ¸ÑÐ½Ð¾Ð³Ð¾ ÑÐ¼ÐµÑÐµÐ½Ð¸Ñ ÐºÐ¾Ð»Ð¾Ð½Ð¸Ð¹ ÑÐ°ÑÑÑÐ¸ÑÐ°Ð½Ñ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÑ Ð´Ð¸ÑÑÑÐ·Ð¸Ð¸ ÐºÐ¾Ð»Ð¾Ð½Ð¸Ð¹ Ð² ÑÐ°Ð¼ÐºÐ°Ñ Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ Ð´ÑÐ¾Ð±Ð½Ð¾Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ ÐÐ»ÐµÐ¹Ð½Ð°âÐÑÐ°Ð¼ÐµÑÑÐ°. ÐÐ¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐµ Ð»Ð¸Ð½Ð¸Ð¸ CaSR Ð¸ H9 ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸Ð·ÑÑÑÑÑ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÐ¼Ð¸ ÐºÐ¾ÑÑÑÐ¸ÑÐ¸ÐµÐ½ÑÐ°Ð¼Ð¸ Ð´Ð¸ÑÑÑÐ·Ð¸Ð¸, Ð¿ÑÐ¸ ÑÑÐ¾Ð¼ ÐºÐ¾Ð»Ð¾Ð½Ð¸Ð¸ Ð¾Ð±ÐµÐ¸Ñ Ð»Ð¸Ð½Ð¸Ð¹ Ð´ÐµÐ¼Ð¾Ð½ÑÑÑÐ¸ÑÑÑÑ ÑÐµÐ¶Ð¸Ð¼ ÑÑÐ¿ÐµÑÐ´Ð¸ÑÑÑÐ·Ð¸Ð¸. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð´Ð°ÑÑ Ð½Ð¾Ð²ÑÑ Ð¸Ð½ÑÐ¾ÑÐ¼Ð°ÑÐ¸Ñ Ð¾Ð± Ð¾ÑÐ¾Ð±ÐµÐ½Ð½Ð¾ÑÑÑÑ ÑÐ¾ÑÑÐ° Ð¸ Ð¼Ð¸Ð³ÑÐ°ÑÐ¸Ð¸ ÐºÐ¾Ð»Ð¾Ð½Ð¸Ð¹ ÑÐ°ÑÑÐ¼Ð¾ÑÑÐµÐ½Ð½ÑÑ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð»Ð¸Ð½Ð¸Ð¹.
description: Understanding the mechanisms governing the growth of human pluripotent stem cell colonies is important for improving the conditions for their cultivation and selection for further use in regenerative medicine. The work is devoted to the study of the growth and migration of human pluripotent stem cell colonies of two cell lines (CaSR and H9). The analysis of phase-contrast images of growing colonies at several times was carried out, the dynamics of colony areas and their trajectories were obtained. The growth parameters are calculated using the exponential growth model of colonies. The analysis of the parameters allowed us to identify a factor that affects the change in colony size and is different from the factors associated with cell division and colony fusion. This factor can be interpreted as a manifestation of intracellular molecular processes regulating the pluripotent phenotype of the colony. As a result of the analysis of the dynamics of the rootâmean-square displacement of colonies, the parameters of colony diffusion are calculated within the framework of a model based on the fractional differential Klein-Kramers equation. It has been shown that the CaSR and H9 cell lines are characterized by different diffusion coefficients, while the colonies of both lines exhibit a superdiffusion regime. The obtained results provide new information about the features of growth and migration of colonies of the considered cell lines.