title: Ð­ÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð³ÐµÐ½Ð° f21d5.3 Ñ Ð½ÐµÐ¼Ð°ÑÐ¾Ð´Ñ caenorhabditis elegans Ñ Ð¼ÑÑÐ°ÑÐ¸ÐµÐ¹ Ð² Ð³ÐµÐ½Ðµ cua-1
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Expression of f21d5.3 gene in nematode Caenorhabditis elegans with mutation in cua-1 gene
Creators: name: Ð¡Ð°Ð¼ÑÑÐµÐ²Ð°, ÐÐ¾Ð»Ð¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¼ Ð¼ÐµÐ´Ð¸
ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð³ÐµÐ½Ð¾Ð²
ÑÐµÑÑÐ»Ð¾Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð½
Ð¼ÑÐ»ÑÑÐ¸Ð¼ÐµÐ´Ð½ÑÐµ Ð¾ÐºÑÐ¸Ð´Ð°Ð·Ñ
copper metabolism
gene expression
ceruloplasmin
multicopper oxidases

description: ÐÐµÐ´Ñ (Cu) â Ð½ÐµÐ·Ð°Ð¼ÐµÐ½Ð¸Ð¼ÑÐ¹ ÐºÐ¾ÑÐ°ÐºÑÐ¾Ñ ÐºÐ»ÑÑÐµÐ²ÑÑ Ð¾ÐºÐ¸ÑÐ»Ð¸ÑÐµÐ»ÑÐ½Ð¾-Ð²Ð¾ÑÑÑÐ°Ð½Ð¾Ð²Ð¸ÑÐµÐ»ÑÐ½ÑÑ ÑÐµÑÐ¼ÐµÐ½ÑÐ¾Ð². ÐÐ´Ð½Ð°ÐºÐ¾, ÑÐ²Ð¾Ð±Ð¾Ð´Ð½ÑÐµ Ð¸Ð¾Ð½Ñ Cu ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ñ ÐºÐ°ÑÐ°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°ÑÑ Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ðµ ÐÐ¤Ð, ÐºÐ¾ÑÐ¾ÑÑÐµ Ð²ÑÐ·ÑÐ²Ð°ÑÑ Ð¾ÐºÐ¸ÑÐ»Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÑÑÐµÑÑ. ÐÐµÐ·Ð¾Ð¿Ð°ÑÐ½ÑÐ¹ Ð¿ÐµÑÐµÐ½Ð¾Ñ Cu Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐµÑ Ð¼ÐµÐ´Ñ-ÑÑÐ°Ð½ÑÐ¿Ð¾ÑÑÐ½Ð°Ñ ÑÐ¸ÑÑÐµÐ¼Ð° (ÐÐ¢Ð¡), Ð²ÑÑÐ¾ÐºÐ¾ ÐºÐ¾Ð½ÑÐµÑÐ²Ð°ÑÐ¸Ð²Ð½Ð°Ñ Ð¾Ñ Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹ Ð´Ð¾ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°. ÐÑÐ½Ð¾Ð²Ñ ÐÐ¢Ð¡, Ð¾ÑÐ²ÐµÑÐ°ÑÑÑÑ Ð·Ð° Ð¸Ð¼Ð¿Ð¾ÑÑ, ÑÐ°ÑÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð¸ Ð²ÑÐ²ÐµÐ´ÐµÐ½Ð¸Ðµ Ð¼ÐµÐ´Ð¸, ÑÐ¾ÑÑÐ°Ð²Ð»ÑÐµÑ Ð¾ÑÑ CTR1-ATOX1-ATP7A/B-CP. ÐÐ»Ñ Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾-Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð¾ÑÐ½Ð¾Ð² Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ð¹, ÑÐ²ÑÐ·Ð°Ð½Ð½ÑÑ Ñ Ð½Ð°ÑÑÑÐµÐ½Ð¸ÐµÐ¼ ÑÐ°Ð±Ð¾ÑÑ ÐÐ¢Ð¡, Ð¸ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ¸ Ð¿ÑÑÐµÐ¹ Ð¸Ñ ÐºÐ¾ÑÑÐµÐºÑÐ¸Ð¸ Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼Ð° Ð±Ð¾Ð»ÐµÐµ Ð¿ÑÐ¾ÑÑÐ°Ñ Ð¼Ð¾Ð´ÐµÐ»Ñ, ÑÐµÐ¼ Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð¼Ð»ÐµÐºÐ¾Ð¿Ð¸ÑÐ°ÑÑÐ¸Ñ, Ð¸ÑÐ¿Ð¾Ð»ÑÐ·ÑÐµÐ¼ÑÐµ Ð² Ð½Ð°ÑÑÐ¾ÑÑÐµÐµ Ð²ÑÐµÐ¼Ñ. Ð ÑÐ°Ð±Ð¾ÑÐµ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ Ð½ÐµÐ¼Ð°ÑÐ¾Ð´Ñ C. elegans, Ð´Ð»Ñ ÐºÐ¾ÑÐ¾ÑÐ¾Ð¹ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ñ Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸ Ð½ÐµÐºÐ¾ÑÐ¾ÑÑÑ Ð³ÐµÐ½Ð¾Ð² ÐÐ¢Ð¡ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°: Ð³ÐµÐ½ chca-1 ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²ÑÐµÑ Ð³ÐµÐ½Ñ CTR1, cuc-1 â ATOX1, cua-1 â ÐÐ¢Ð 7Ð/Ð. ÐÑÐµÐ´Ð¿Ð¾Ð»Ð°Ð³Ð°ÐµÐ¼ÑÐ¼ Ð³Ð¾Ð¼Ð¾Ð»Ð¾Ð³Ð¾Ð¼ Ð³ÐµÐ½Ð° CP ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° ÑÐ²Ð»ÑÐµÑÑÑ f21d5.3, ÐºÐ¾ÑÐ¾ÑÑÐ¹ ÐºÐ¾Ð´Ð¸ÑÑÐµÑ Ð¼ÑÐ»ÑÑÐ¸Ð¼ÐµÐ´Ð½ÑÑ Ð¾ÐºÑÐ¸Ð´Ð°Ð·Ñ (ÐÐÐ) C. elegans, Ð½Ð¾ Ð½Ð°Ð»Ð¸ÑÐ¸Ðµ Ñ Ð½ÐµÐ¼Ð°ÑÐ¾Ð´Ñ ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²ÑÑÑÐµÐ³Ð¾ Ð±ÐµÐ»ÐºÐ° Ð½Ðµ Ð¿Ð¾Ð´ÑÐ²ÐµÑÐ¶Ð´ÐµÐ½Ð¾. Ð¦ÐµÐ»Ñ ÑÐ°Ð±Ð¾ÑÑ â Ð¸Ð·ÑÑÐ¸ÑÑ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ f21d5.3 C. elegans. Ð ÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð±Ð¸Ð¾Ð¸Ð½ÑÐ¾ÑÐ¼Ð°ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð°Ð¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾ÑÐ½Ð¾Ð¹ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÐ¸ Ð±ÐµÐ»ÐºÐ° ÑÐºÐ°Ð·ÑÐ²Ð°ÑÑ Ð½Ð° ÑÐ¾, ÑÑÐ¾ F21D5.3 ÑÐ²Ð»ÑÐµÑÑÑ ÐÐÐ. Ð­ÐºÑÐ¿ÑÐµÑÑÐ¸Ñ f21d5.3 Ð¿Ð¾Ð´ÑÐ²ÐµÑÐ¶Ð´ÐµÐ½Ð° Ð¸ Ð¸Ð·Ð¼ÐµÐ½ÑÐµÑÑÑ Ð² Ð¾ÑÐ²ÐµÑ Ð½Ð° Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ Ð³Ð¾Ð¼ÐµÐ¾ÑÑÐ°Ð·Ð° Ð¼ÐµÐ´Ð¸ Ð² Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ðµ Ð½ÐµÐ¼Ð°ÑÐ¾Ð´Ñ. ÐÐµÐ»Ð¾Ðº F21D5.3 ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐµÑÐºÐ¸ ÑÐ²ÑÐ·ÑÐ²Ð°ÐµÑÑÑ Ñ Ð°Ð½ÑÐ¸ÑÐµÐ»Ð°Ð¼Ð¸ Ðº Ð¦Ð Ð¼ÑÑÐ¸ Ð¸ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¾Ð±Ð»Ð°Ð´Ð°ÐµÑ Ð¾ÐºÑÐ¸Ð´Ð°Ð·Ð½Ð¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑÑ. ÐÐ±ÑÑÐ¶Ð´Ð°ÐµÑÑÑ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ°Ñ ÑÐ¾Ð»Ñ Ð±ÐµÐ»ÐºÐ° F21D5.3 Ð¸ ÐµÐ³Ð¾ Ð·Ð½Ð°ÑÐµÐ½Ð¸Ðµ Ð´Ð»Ñ ÐÐ¢Ð¡ Ð½ÐµÐ¼Ð°ÑÐ¾Ð´Ñ C. elegans.
description: Copper (Cu) is an essential cofactor for key redox enzymes. However, free Cu ions can catalyze formation of reactive oxygen species (ROS) inducing oxidative stress. The safe transport of Cu is mediated by the copper transport system (CTS), which is highly conserved from yeast to human. The CTR1-ATOX1-ATP7A/B-CP axis forms the backbone of the CTS responsible for the import, distribution, and excretion of copper. A simpler model than the mammalian models currently used is needed to study the molecular genetic basis of CTS dysfunction associated diseases and for development of new therapeutic approaches. In the present work, we used nematode C. elegans, for which the orthologs of some human CTS genes have been identified: chca-1 is homologous to CTR1, cuc-1â to ATOX1, cua-1 â to ATR7A/B. The putative homologue of the human CP is C. elegans f21d5.3, which encodes a multicopper oxidase (MCO), but the presence of the corresponding protein in the nematode has not been confirmed. The aim of this work is to study the expression of f21d5.3 in C. elegans. Results of bioinformatic analysis of F21D5.3 amino acid sequence identifies the protein as MCO. Expression of f21d5.3 is confirmed and changes in response to alterations in nematode copper homeostasis. F21D5.3 binds specifically to mouse and human CP antibodies and has oxidase activity. The biological role of F21D5.3 and its significance for C. elegans CTS are discussed.