Preparation of nanolignin rich fraction from bamboo stem via green technology: assessment of its antioxidant, antibacterial and UV blocking properties
Creators: name: Mili, Medha
name: Hashmi, S. A. R.
name: Tilwari, Anita
name: Rathore, S. K. S.
name: Naik, Ajay
name: Srivastava, A. K.
name: Verma, Sarika
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Pharmacology
subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Cancer
subject: Hematology

description: This work reports the preparation of nano lignin-rich fraction material via green technology from the holistic use of lignocellulosic biomass bamboo. The bamboo is first chemically treated, followed by acid precipitation to extract bamboo-derived macro lignin-rich fraction material. The nano lignin-rich fraction material was then prepared via ultrasonication technique from the extracted bamboo-derived macro lignin-rich fraction material. The confirmation of the distinct lignin functional groups in the extracted lignin-rich fractions has been done by FTIR. Surface morphology by FESEM and TEM revealed spherical nano-lignin-rich fraction materials from extracted bamboo-derived macro lignin-rich fraction materials. DPPH assays indicated that both the obtained fractions depict beneficial antioxidant characteristics. They were found to be effective in terms of their antibacterial activity against both gram-positive bacteria <i>Staphylococcus aureus (S.aureus)</i> and gram-negative bacteria <i>Escherichia coli (E.coli)</i>, using the disc diffusion method. These fractions have UV blocking property, and nano-lignin-rich fraction material acts as a more potential UV blocking agent than others. Thus, the nano-lignin-rich fraction material has great potential as a high antioxidant, antibacterial, and UV blocking agent useful in biomedical applications. <b>Highlights</b>Extraction of macro-lignin rich fraction material using chemical treatment of lignocellulosic biomass bamboo via refluxing followed by acid precipitation.Preparation of nano-lignin rich fraction material from extracted bamboo-derived macro-lignin rich fraction material via ultrasonication technique as a green technology.Structural and surface morphology of the extracted macro-lignin &amp; nano lignin-rich fraction materials have been analyzed by XRD, FTIR, EDX, SEM and TEM.The macro lignin &amp; nano lignin-rich fraction materials showed good antioxidant, antibacterial activity and UV-blocking properties, but the nano-lignin rich fraction material exhibited more efficient properties. Extraction of macro-lignin rich fraction material using chemical treatment of lignocellulosic biomass bamboo via refluxing followed by acid precipitation. Preparation of nano-lignin rich fraction material from extracted bamboo-derived macro-lignin rich fraction material via ultrasonication technique as a green technology. Structural and surface morphology of the extracted macro-lignin &amp; nano lignin-rich fraction materials have been analyzed by XRD, FTIR, EDX, SEM and TEM. The macro lignin &amp; nano lignin-rich fraction materials showed good antioxidant, antibacterial activity and UV-blocking properties, but the nano-lignin rich fraction material exhibited more efficient properties.