Additional file 1 of Exogenous melatonin enhances the growth and production of bioactive metabolites in Lemna aequinoctialis culture by modulating metabolic and lipidomic profiles
Creators: name: Baek, GahYoung
name: Lee, Hwanhui
name: Ko, JuHee
name: Choi, Hyung-Kyoon
Publisher: name: figshare
Year: 2022
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Pharmacology
subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: Inorganic Chemistry
subject: Plant Biology
subject: Computational Biology

description: Additional file 1: Figure S1. Growth of L. aequinoctialis culture cultivated for 35 days. Total number offronds (A) and dry weight (B).Dataare mean values, and the vertical bars represent the standard deviation fromthree biological replications. TableS1.Identification of various L.aequinoctialis culture metabolites by gas chromatography-mass spectrometry(GC-MS) analysis.The base peak ineach compound among ion fragments is shown in bold characters. RT, retentiontime; TMS, trimethylsilylation; MEOX, methoxylamine hydrochloride. TableS2 Relativelevels (relative intensity/g) of various L.aequinoctialis culture metabolites cultivated under various melatoninconcentrations at day 14.Data aremean ± standard deviationvalues of nine measurements from three biological replicates and threetechnical replicates of the control group and the 0.1, 1, and 10 μM melatonin treatment groups on day 14. The valueswithin a column with different letters (a, b, and c) mean statisticallysignificant differences evaluated by using the Kruskal-Wallis test followed by theMann-Whitney test as a post hoc analysis with Bonferroni's correction (p &lt; 0.0083). TableS3.Relative levels (relative intensity/g) of various L. aequinoctialis culture metabolites cultivated under variousmelatonin concentrations at day 28.Dataare mean ± standard deviation values of nine measurements from three biologicalreplicates and three technical replicates of the control group and the 0.1, 1,and 10 μM melatonin treatment groups on day 28. The values within a column withdifferent letters (a, b, and c) mean statistically significant differencesevaluated by using the Kruskal-Wallis test followed by the Mann-Whitney test asa post hoc analysis with Bonferroni's correction (p &lt; 0.0083). TableS4.Identification of various intact lipid species in L. aequinoctialis culture by nano-electrospray ionization-mass spectrometry(nanoESI-MS) analysis. TableS5 Relativelevels (relative intensity/g) of various intact lipid species of L. aequinoctialis culture cultivatedunder various melatonin concentrations at day 14. Data are mean ± standarddeviation values of nine measurements from three biological replicates and threetechnical replicates of the control group and the 0.1, 1, and 10 μM melatonintreatment groups on day 14. The values within a column with different letters(a, b, and c) mean statistically significant differences evaluated by using theKruskal-Wallis test followed by the Mann-Whitney test as a post hoc analysiswith Bonferroni's correction (p &lt; 0.0083). TableS6.Relative levels (relative intensity/g) of various intact lipid species of L. aequinoctialis culturecultivated under various melatoninconcentrations at day 28.Data aremean ± standard deviationvalues of 9 measurements from 3 biological replicates and 3 technicalreplicates of control group and 0.1 μM, 1 μM, and 10 μMmelatonin treatment groups on day 28. The values within a column withthe different letters (a, b, and c) mean statistically significant differencesevaluated byusing the Kruskal-Wallis test followed by Mann-Whitney test as post hocanalysis with Bonferroni's correction (p &lt;0.0083).