cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Lethal giant larvae 1 tumour suppressor activity is not conserved in models of mammalian T and B cell leukaemia
Creators: name: Hawkins, Edwin D.
name: Oliaro, Jane
name: Ramsbottom, Kelly M.
name: Ting, Stephen B.
name: Sacirbegovic, Faruk
name: Harvey, Michael
name: Kinwell, Tanja
name: Ghysdael, Jacques
name: Johnstone, Ricky W.
name: Humbert, Patrick O.
name: Russell, Sarah
Publisher: name: Swinburne
Year: 2024
Subjects: classificationCode: 310102
subject: Cell development, proliferation and death
classificationCode: 510302
subject: Classical and physical optics

description: In epithelial and stem cells, lethal giant larvae (Lgl) is a potent tumour suppressor, a regulator of Notch signalling, and a mediator of cell fate via asymmetric cell division. Recent evidence suggests that the function of Lgl is conserved in mammalian haematopoietic stem cells and implies a contribution to haematological malignancies. To date, direct measurement of the effect of Lgl expression on malignancies of the haematopoietic lineage has not been tested. In Lgl1-/- mice, we analysed the development of haematopoietic malignancies either alone, or in the presence of common oncogenic lesions. We show that in the absence of Lgl1, production of mature white blood cell lineages and long-term survival of mice are not affected. Additionally, loss of Lgl1 does not alter leukaemia driven by constitutive Notch, c-Myc or Jak2 signalling. These results suggest that the role of Lgl1 in the haematopoietic lineage might be restricted to specific co-operating mutations and a limited number of cellular contexts.
Key Amino Acids in the Bacterial (6-4) Photolyase PhrB from Agrobacterium fabrum
Creators: name: Graf, Dominik
name: Wesslowski, Janine
name: Ma, Hongju
name: Scheerer, Patrick
name: Krauß, Norbert
name: Oberpichler, Inga
name: Zhang, Fan
name: Lamparter, Tilman
Publisher: name: Charité - Universitätsmedizin Berlin
Year: 2015
Subjects: subject: 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit

description: Photolyases can repair pyrimidine dimers on the DNA that are formed during UV irradiation. PhrB from Agrobacterium fabrum represents a new group of prokaryotic (6–4) photolyases which contain an iron-sulfur cluster and a DMRL chromophore. We performed site-directed mutagenesis in order to assess the role of particular amino acid residues in photorepair and photoreduction, during which the FAD chromophore converts from the oxidized to the enzymatically active, reduced form. Our study showed that Trp342 and Trp390 serve as electron transmitters. In the H366A mutant repair activity was lost, which points to a significant role of His366 in the protonation of the lesion, as discussed for the homolog in eukaryotic (6–4) photolyases. Mutants on cysteines that coordinate the Fe-S cluster of PhrB were either insoluble or not expressed. The same result was found for proteins with a truncated C-terminus, in which one of the Fe-S binding cysteines was mutated and for expression in minimal medium with limited Fe concentrations. We therefore assume that the Fe-S cluster is required for protein stability. We further mutated conserved tyrosines that are located between the DNA lesion and the Fe-S cluster. Mutagenesis results showed that Tyr424 was essential for lesion binding and repair, and Tyr430 was required for efficient repair. The results point to an important function of highly conserved tyrosines in prokaryotic (6–4) photolyases.
Cost-effectiveness of tiotropium versus omalizumab for uncontrolled allergic asthma in US
Creators: name: Zafari, Zafar
name: Sadatsafavi, Mohsen
name: Mark FitzGerald, J.
Publisher: name: BioMed Central
Year: 2018

description: Background: A significant minority of asthma patients remain uncontrolled despite the use of inhaled corticosteroids (ICS) and long-acting beta-agonists (LABA). A number of add-on therapies, including monoclonal antibodies (namely omalizumab) and more recently tiotropium bromide have been recommended for this subgroup of patients. The purpose of this study was to assess the cost-effectiveness of tiotropium versus omalizumab as add-on therapies to ICS + LABA for patients with uncontrolled allergic asthma. Methods: A probabilistic Markov model of asthma was created. Total costs (in 2013 US $) and health outcomes of three interventions including standard therapy (ICS + LABA), add-on therapy with tiotropium, and add-on therapy with omalizumab, were calculated over a 10-year time horizon. Future costs and quality-adjusted life years (QALYs) were discounted at the rate of 3%. Multiple sensitivity analyses were conducted. Cost-effectiveness was evaluated at willingness-to-pay value of $50,000. Results: The 10-year discounted costs and QALYs for standard therapy were $38,432 and 6.79, respectively. The corresponding values for add-on therapy with tiotropium and with omalizumab were $41,535 and 6.88, and $217,847 and 7.17, respectively. The incremental cost-effectiveness ratios (ICER) of add-on therapy with tiotropium versus standard therapy, and omalizumab versus tiotropium were $34,478/QALY, and $593,643/QALY, respectively. The model outcomes were most sensitive to the costs of omalizumab but were robust against other assumptions. Conclusions: Although omalizumab had the best health outcomes, add-on therapy with tiotropium was a cost-effective alternative to omalizumab and standard therapy for uncontrolled allergic asthma at willingness-to-pay of $50,000/QALY.
The role of tyrosine kinase in the contraction of rat mesenteric artery.
Creators: name: Birnbaum, Ellen Sharon.
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 1998
Subjects: subject: Health Sciences, Pharmacology.

description: The phosphorylation of proteins on tyrosine residues by tyrosine kinases has been suggested to contribute to signalling processes that lead to smooth muscle contraction. The aim of the current study was to further determine the role of tyrosine kinase in the contraction of rat vascular smooth muscle. The specific objectives included (i) studying the effect of tyrosine kinase inhibitors on vascular contraction induced by noradrenaline (NA), potassium chloride (KCl) and neuropeptide V (NPY); (ii) studying the effect of sodium orthovanadate on contraction; (iii) studying the role of endothelium dependence in contraction; as well as (iv) studying the role of L-type Ca$\sp{2+}$ channels in the tyrosine kinase-related response. The effects of NPY, nifedipine, sodium orthovanadate and various tyrosine kinase inhibitors were tested in intact ring segments of rat mesenteric and tail arteries. The present studies illustrate that tyrosine kinase plays an important role in signal transduction stimulated by agonists in vascular smooth muscle. (Abstract shortened by UMI.)
Photochemistry of amido xanthonate photocages: Potential candidates for polymer-tethered photorelease of drugs
Creators: name: Yorke, Matthew
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2010
Subjects: subject: Chemistry, Organic.
subject: Chemistry, Pharmaceutical.

description: Recently, photocages capable of releasing biologically relevant molecules have been reported, which utilize the rapid and efficient photodecarboxylation of 2-xanthone acetic acid (XAA) with UVA irradiation. This thesis focuses on the development of functionalized xanthonate photocages to be used for surface-tethering applications. Firstly, the preparation of amine and acetamide functionalized xanthonate (and thioxanthonate) photocage precursors will be described. Investigation into the photochemistry of the prepared derivatives led to the discovery of a solvent composition dependence on the photodecarboxylation quantum yield of acetamide derivatives. The photodecarboxylation of these derivatives occurs cleanly in the same way as XAA, but with lower efficiency. The preparation and photochemical study of acetamide functionalized photocages will then be described. Photorelease of acetate and phenylalanine demonstrated the capability of amide functionalized photocages to release biologically relevant molecules. Lastly, initial investigations into polymer tethering will be described. Attempted amide coupling through the aromatic amine led to the attachment of a short chain carboxylic acid terminated linker to modify the linking site.
3536263.pdf
Creators: name: Rolland, Antoine
name: Li, Peng
name: Kuse, Naoya
name: Jiang, Jie
name: Cassinerio, Marco
name: Langrock, Carsten
name: Fermann, Martin
Publisher: name: Optica Publishing Group
Year: 2018
Subjects: Uncategorized

description: Revised supplemental material
3567823.pdf
Creators: name: Giacoumidis, Elias
name: Choudhary, Amol
name: Magi, Eric
name: Marpaung, David
name: Vu, Khu
name: Ma, Pan
name: Choi, Duk-Yong
name: Madden, Stephen
name: Corcoran, Bill
name: Pelusi, Mark
Publisher: name: Optica Publishing Group
Year: 2018
Subjects: Uncategorized

description: Supplemental document
Synthesis of sialic acid antigens.
Creators: name: Laferrière, Craig A.
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 1990
Subjects: subject: Chemistry, Pharmaceutical.

description: N-Acetylneuraminic acid (NeuAc) is a sialic acid which constitutes terminal positions in glycoproteins and glycolipids. It is part of the antigenic determinant of many forms of cancer (S. Hakomori, Chem. Phys. Lipids. 42, 209 (1986)) and hence a cancer "vaccine" could be made from an appropriate multivalent macromolecular form of NeuAc. To explore the possibility of inducing anti-NeuAc antibodies, we have synthesized polyvalent conjugates of $\alpha$NeuAc or NeuAc$\alpha$2-3Gal$\beta$1-4Glc (sialyl2-3lactose) on protein carriers. This was accomplished by reductive amination with 2-oxoethyl $\alpha$-NeuAc or with the concealed aldehyde of the reducing sugar (glucose) onto the lysine residues of the proteins Bovine Serum Albumin (BSA) and Tetanus Toxoid (TT). A new procedure involving a Michael-type addition employed the $\epsilon$-amino groups of the lysine residues in a 1,4 nucleophilic addition to the acrylamide functional group in two derivatives of NeuAc; the N-acryloylaminoethylthiopropyl glycoside, and the N-acryloylsialyl2-3lactosylamine. This technique does not require the use of reagents and was performed under mild basic conditions. Polyacrylamide copolymers were also synthesized by radical co-polymerization of acrylamide with NeuAc monomers, providing a polymer (Mw $\approx$ 100 kDa) with pendant NeuAc. Reactive monomers giving different spacer arms between the sugar and the polymer backbone were developed, including the allyl glycoside, a more reactive N-acryloylaminoethylthiopropyl glycoside and several lactose derivatives. The polymers were found to have a better shelf life than the proteins, and had the advantage of sharing only the NeuAc moiety. Some of the NeuAc/protein conjugates were used to immunize rabbits, and the antibodies formed were screened with polyacrylamide copolymers with pendant NeuAc. The polymer with spacer was found to react better with the antibodies in immunoprecipitation and enzyme-linked immunosorbent assay (ELISA). Inhibition of ELISA experiments were done using synthesized derivatives of NeuAc to determine the binding specificity of the antibodies. It was found that the antibodies recognized the glycerol side chain, acid function, spacer, and to a lesser extent, the acetamido function of NeuAc. The same methodology was used to map the binding site of the lectin from Triticum vulgaris (called Wheat germ agglutinin, WGA) which binds NeuAc. Nuclear magnetic resonance (nmr) studies were undertaken to determine the acidity constants (pK$\sb{\rm a}$) and conformation of several NeuAc derivatives. Also studied was the lactone of NeuAc$\alpha$2-3Gal$\beta$1-4Glc which is believed to be responsible for the immunogenicity of certain cancer cells (T. Dohi, G. Nores and S. Hakomori, Cancer Res. 48, 5680 (1988)). This work was complemented by molecular modelling using MM2. A model was proposed for the conformation of the lactone which is consistent with the n.m.r. evidence.
Design and study of a free-space quasi-optical measurement system.
Creators: name: Gagnon, Nicolas.
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2002
Subjects: subject: Engineering, Electronics and Electrical.

description: This thesis describes a free-space quasi-optical measurement system designed to characterise dielectric materials at Ka band. The measurement system consists of a pair of colinear horn-fed lenses between which the sample under test is inserted. A study is performed in order to determine the sensitivity of the structure to misalignments, which reveals that care must be taken to ensure constant exact positioning of the components of the measurement system. Reflection and transmission coefficients (S-parameters) of the sample are obtained using a two-port vector network analyser connected to the feed horns. Calibration of the system is performed using a free-space through-reflect-match (TRM) technique, which is preferred over other calibration techniques because it does not require the movement of any component of the system during the calibration process. Correction terms must be applied to the reflection and transmission coefficients because of errors due to the misplacement of the sample and the calibration procedure. The sample's dielectric constant is then extracted from the corrected reflection and transmission coefficients using one of two different numerical techniques: a root-finding algorithm and a genetic algorithm. Results obtained with both extraction techniques were found to be similar. Moreover, these results are in good agreement with published manufacturer data and with measurements performed by an independent organisation using a resonance technique. This work demonstrates the feasibility of a low-cost, functional and flexible quasioptical measurement system for characterising dielectric materials at Ka band.
Medium Cut-Off (MCO) Membranes Reduce Inflammation in Chronic Dialysis Patients—A Randomized Controlled Clinical Trial
Creators: name: Zickler, Daniel
name: Schindler, Ralf
name: Willy, Kevin
name: Martus, Peter
name: Pawlak, Michael
name: Storr, Markus
name: Hulko, Michael
name: Boehler, Torsten [U.A.]
Publisher: name: Charité - Universitätsmedizin Berlin
Year: 2017
Subjects: subject: 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit

description: Background To increase the removal of middle-sized uremic toxins a new membrane with enhanced permeability and selectivity, called Medium Cut-Off membrane (MCO-Ci) has been developed that at the same time ensures the retention of albumin. Because many middle-sized substances may contribute to micro-inflammation we hypothesized that the use of MCO-Ci influences the inflammatory state in hemodialysis patients. Methods The randomized crossover trial in 48 patients compared MCO-Ci dialysis to High-flux dialysis of 4 weeks duration each plus 8 weeks extension phase. Primary endpoint was the gene expression of TNF-α and IL-6 in peripheral blood mononuclear cells (PBMCs), secondary endpoints were plasma levels of specified inflammatory mediators and cytokines. Results After four weeks of MCO-Ci the expression of TNF-α mRNA (Relative quantification (RQ) from 0.92 ± 0.34 to 0.75 ± 0.31, -18.5%, p<0.001)-α and IL-6 mRNA (RQ from 0.78 ± 0.80 to 0.60 ± 0.43, -23.1%, p<0.01) was reduced to a significantly greater extent than with High-flux dialyzers (TNF mRNA-RQ: -14.3%; IL-6 mRNA-RQ: -3.5%). After retransformation of logarithmically transformed data, measurements after MCO were reduced to 82% of those after HF (95% CI 74%–91%). 4 weeks use of MCO-Ci resulted in long- lasting change in plasma levels of several cytokines and other substances with a significant decrease for sTNFR1, kappa and lambda free light chains, urea and an increase for Lp-PLA2 (PLA2G7) compared to High-flux. Albumin levels dropped significantly after 4 weeks of MCO dialysis but increased after additional 8 weeks of MCO dialysis. Twelve weeks treatment with MCO-Ci was well tolerated regarding the number of (S)AEs. In the extension period levels of CRP, TNF-α-mRNA and IL-6 mRNA remained stable in High-flux as well as in MCO-Ci. Conclusions MCO-Ci dialyzers modulate inflammation in chronic HD patients to a greater extent compared to High-flux dialyzers. Transcription of pro-inflammatory cytokines in peripheral leukocytes is markedly reduced and removal of soluble mediators is enhanced with MCO dialysis. Serum albumin concentrations stabilize after an initial drop. These results encourage further trials with longer treatment periods and clinical endpoints.
Anticipating the potential for positive uptake and adaptation in the implementation of a publicly funded online STBBI testing service: a qualitative analysis
Creators: name: Chabot, Cathy
name: Gilbert, M.
name: Haag, Devon
name: Ogilvie, Gina
name: Hawe, Penelope
name: Bungay, Vicky
name: Shoveller, Jean
Publisher: name: BioMed Central
Year: 2018

description: Background: Online health services are a rapidly growing aspect of public health provision, including testing for sexually transmitted and other blood-borne infections (STBBI). Generally, healthcare providers, policymakers, and clients imbue online approaches with great positive potential (e.g., encouraging clients’ agency; providing cost-effective services to more clients). However, the promise of online health services may vary across contexts and be perceived in negative or ambiguous ways (e.g., risks to ‘gold standard’ care provision; loss of provider control over an intervention; uncertainty related to budget implications). This study examines attitudes and perceptions regarding the development of a novel online STBBI testing service in Vancouver, Canada. We examine the perceptions about the intervention’s potential by interviewing practitioners and planners who were engaged in the development and initial implementation of this testing service. Methods: We conducted in-depth interviews with 37 healthcare providers, administrators, policymakers, and community-based service providers engaged in the design and launch of the new online STBBI testing service. We also conducted observations during planning and implementation meetings for the new service. Thematic analysis techniques were employed to identify codes and broader discursive themes across the interview transcripts and observation notes. Results: Some study participants expressed concern that the potential popularity of the new testing service might increase demand on existing sexual health services or become fiscally unsustainable. However, most participants regarded the new service as having the potential to improve STBBI testing in several ways, including reducing waiting times, enhancing privacy and confidentiality, appealing to more tech-savvy sub-populations, optimizing the redistribution of demands on face-to-face service provision, and providing patient-centred technology to empower clients to seek testing. Conclusions: Participants perceived this online STBBI testing service to have the potential to improve sexual health care provision. But, they also anticipated actions-and-reactions, revealing a need to monitor ongoing implementation dynamics. They also identified the larger, potentially system-transforming dimension of the new technology, which enables new system drivers (consumers) and reduces the amount of control health care providers have over online STBBI testing compared to conventional in-person testing.
After dark
Creators: name: Stark, Liv
Publisher: name: University of Iowa
Year: 2023
Subjects: Alternative process
Cyanotype
Illustration
Nocturnal pollinators
Van Dyke Brown
public abstract
Photography

description: Within nature, a delicate balance exists between flora and fauna that is at once threatened and misunderstood. Despite many plant and animal populations being on the decline, not all populations receive the same amount of attention. Out of this concern, this work evaluates the less-popular species facing population decline, such as nocturnal pollinators like moths and bats, and investigates how to make their importance more accessible.
description: Several various processes were employed to discover unique ways to depict nocturnal pollinators, from lumen prints and cyanotypes to illustrations and a combination of the few. The lumen print and cyanotype processes are considered alternative photographic processes, allowing centuries-old techniques to inform contemporary work and problems. These non-traditional methods allow nature to play a role in the production of these images, whether by the sun exposing the prints or the inclusion of the subjects themselves in the process. Alternative processes are also dependent on light and time, much like the fauna that work the night shift.
description: The variation in the resulting works became evocative of the variation that already exists in nature. Not one print looks identical to another, creating an interest in both the diversity throughout the work and within nature. Arranging aesthetically pleasing compositions that romanticize plant-pollinator relationships creates an access point for viewers to engage with the topic.
description: This body of work reveals a need for societal attention on nocturnal pollinators, especially up against negative misconceptions regarding these species. It simultaneously asks the viewer to consider the fragility of life, not just among plants and animals but human beings as well. Empathy fueled by looming death and fear offer the viewer another perspective on the other types of life we share the planet with.
The transmission line matrix (TLM) method and its boundary treatments.
Creators: name: Chen, Zhizhang.
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 1992
Subjects: subject: Engineering, Electronics and Electrical.

description: The Transmission Line Matrix (TLM) numerical algorithm, based on the discrete Huygens' principle, has been extensively used to solve electromagnetic structure problems. The major advantage of this method is its simplicity and flexibility as the vectorial Maxwell's Equations are transformed into a simple numerical model of digital signal processing system. In this thesis, new and efficient numerical modeling concepts and procedures have been developed for the analysis of electromagnetic structures with the TLM method: (1) With the introduction of the equivalent field quantities defined between nodes, the TLM Method has been shown to be exactly equivalent to a finite-difference timedomain (FD-TD) formulation. Therefore, the numerical foundation of the TLM approach has been fully demonstrated and the basis for mathematically understanding the TLM method has been provided. As a result, the conventional TLM boundary conditions has been verified theoretically, and hence a systematic way for constructing the TLM boundary conditions has been developed. In addition, a new boundary description for the TLM method has been proposed, which renders TLM method more flexibility in its boundary treatments. (2) Based on the equivalence between the TLM method and the FD-TD method, an absorbing and a connecting boundary formulations have been developed for TLM simulations. With these formulations, the TLM method can be applied for solving more realistic scattering and radiation problems with open structures. The computation examples given in this thesis are with the structures of waveguides, two-dimensional and three-dimensional obstacles illuminated by plane waves. The numerical results show good agreement with those obtained with the Method of Moment, and thus validate the boundary conditions developed. (3) By using the discrete Fourier Transform, a new algorithm has been developed for interfacing the TLM method with the frequency-domain solutions. The technique employ the prior knowledge of frequency-domain solutions at boundaries and combine them with TLM simulations, leading to considerable decrease in memory and CPU time. It also allows the TLM method to be used with highly conductive materials for solving shielding problems. The good results were obtained with significant reduction of the computation expenditure.
title: Vascular occlusions following ocular surgical procedures
title: a clinical observation of vascular complications after ocular surgery
titleType: Subtitle
Creators: name: Fischer, Charlotte
name: Bruggemann, Anne
name: Hager, Annette
name: Planas, Josep Callizo
name: Roider, Johann
name: Hoerauf, Hans
Publisher: name: Charité - Universitätsmedizin Berlin
Year: 2017
Subjects: subject: ischemic optic neuropathy
subject: retinal artery-occlusion
subject: vitreoretinal surgery
subject: intraocular-pressure
subject: retrobulbar anesthesia
subject: risk-factors
subject: blood-flow
subject: gases
subject: 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit

description: Background. Ocular vascular occlusions following intraocular procedures are a rare complication. We report a case series of patients with retinal vascular occlusions or anterior ischemic optic neuropathy (AION) after anterior and posterior segment surgery and demonstrate possible risk factors. Methods. Observational case series. Results. In ten patients, vascular occlusions were observed within ten weeks after intraocular surgery: branch retinal arterial occlusion (BRAO) (n = 2), central retinal artery occlusion (CRAO) (n = 2), central retinal vein occlusion (CRVO) (n = 1), branch retinal vein occlusion (BRVO) (n = 1), anterior ischemic optic neuropathy (AION) (n = 3), and combined central artery and vein occlusion (n = 1). AION occurred later (27–69 d) than arterial occlusions (14–60 d) or venous occlusions (1-2 d). In all cases, either specific surgical manipulations or general vascular disorders were identified as risk factors. In addition to general cardiovascular risk factors (arterial hypertension n = 6, diabetes mellitus n = 4), internal workup disclosed bilateral stenosis of the carotid arteries (n = 1) and myeloproliferative syndrome (n = 1). Conclusion. Vascular occlusions after surgical ocular procedures seem to be more frequent when cardiovascular diseases coexist. Surgical maneuvers and intra- or postoperative pressure changes may act as a triggering mechanism in patients with underlying systemic cardiovascular disorders. Affected patients should undergo thorough internal examination to identify possible underlying diseases.
Conformational effects of lipids and local anesthetics on the nicotinic acetylcholine receptor.
Creators: name: Ryan, Stephen E.
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2000
Subjects: subject: Chemistry, Biochemistry.

description: The mechanisms by which both lipids and local anesthetics modulate the structure and function of the nicotinic acetylcholine receptor (nAChR) from Torpedo have been investigated using the technique of Fourier transform infrared (FTIR) difference spectroscopy. With respect to lipids, the results reveal that reconstitution of the nAChR into a highly fluid egg phosphatidy1choline (EPC) membrane stabilizes the receptor in a channel-inactive desensitized state. The addition of a variety of neutral and/or anionic lipids to the EPC membrane all induce an essentially identical pattern of vibrational changes in the nAChR suggesting that the presence of diverse lipid structures within a reconstituted membrane acts to stabilize the receptor in a channel-competent resting state. In addition, increasing levels of either the neutral lipid, cholesterol (Chol) or the anionic lipid, dioleoylphosphatidic acid (DOPA) within an EPC membrane increasingly stabilizes a larger proportion of nAChR's in a conformation(s) that is (are) capable of undergoing agonist-induced desensitization, although only high levels of DOPA were found to stabilize the receptor in a fully functional resting state. The results have been interpreted in terms of a speculative model that describes the mechanisms by which lipids modulate nAChR function. This model suggests that the composition of the lipid membrane surrounding the nAChR influences the conformational equilibrium of the receptor between the resting, desensitized and a novel intermediate state through an indirect effect on some physical property of the lipid bilayer, possibly bulk fluidity. The model also proposes that anionic lipids, in addition to Proper membrane fluidity, are required to stabilize the nAChR in a fully functional resting conformation. With respect to local anesthetics, the results support the current model of local anesthetic action at the nAChR and suggest that the binding of local anesthetics stabilize the receptor in a desensitized conformation. Contrary to the model, however, the FTIR data suggest that receptor desensitization occurs not through interactions at a distinct noncompetitive blocker site, but solely as a consequence of local anesthetic binding to the neurotransmitter binding sites. The data also suggest that the binding of local anesthetics to the non-competitive blocker site stabilizes the nAChR in an intermediate conformation similar to that stabilized upon reconstitution of the nAChR into EPC membranes containing low concentrations of either Chol or DOPA. Additional studies employing the local anesthetic, proadifen and the hallucinogen, phencyclidine hydrochloride (PCP) support these findings and show conclusively that both local anesthetics and PCP bind to the non-competitive blocker site and stabilize the nAChR in a conformation that is structurally distinct from both the resting and desensitized states.
3585244.pdf
Creators: name: Gao, Dingshan
name: Li, Shuyi
name: Zhou, Yangyang
name: Dong, Jianji
name: Zhang, Xinliang
name: Cassan, Eric
name: Hou, Jin
name: Yang, Chunyong
name: Chen, Shaoping
name: Chen, Huanyang
Publisher: name: Optica Publishing Group
Year: 2025
Subjects: Uncategorized

description: Supplement 1 for the article
Questioning psychological health: Contextualizing contemporary psychological theory through historical dialogue between theologians and psychologists, 1940--1960
Creators: name: Sykes, Britt-Mari
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2006
Subjects: subject: Religion, General.
subject: Theology.
subject: Psychology, Clinical.

description: The thesis explores a dialogue between psychology and religion around the large question of what constitutes psychological health and well-being. It does so by examining three historical---but virtually unknown---illustrations of interdisciplinary dialogue between theologians and psychologists that took place in the United States from 1940--1960 and then by looking at two more contemporary theoretical voices within the discipline of psychology. The thesis contends that the questions and concerns raised by mid-twentieth century psychologists and theologians continue to resonate deeply within contemporary debate about care (both of ourselves and others), psychological health and fulfillment, and the well-being of society generally. The three historical illustrations used in the thesis are the minutes of and paper presentations at the New York Psychology Group (1941-1945), the lecture series of existential psychoanalyst Viktor Frankl at Harvard Divinity School in 1957 and the Harvard Project on Religion and Mental Health directed by Hans Hofmann from 1957--1961. In all three cases, proponents challenged the social sciences, criticized what they saw as psychological scientism, and desired to enlarge psychology's view of human existence and make it one of relationality, social context and ethics. They saw the individual as both a receptive and responsive agent in particular cultural, economic, political and religious contexts, ethically drawn to contribute to, and shape, those contexts. In light of these historical dialogues, the thesis then addresses similar issues in the post-modern reflections of critical psychology and introduces the contemporary theory and therapy of Existential Analysis and suggests its potential role in linking historical and contemporary dialogue between psychology and religion.
title: Me too reframing women’s desire in the movies
title: an analysis of worst person in the world and bad luck banging or loony porn
titleType: Subtitle
Creators: name: Bowden, Samuel
Publisher: name: University of Iowa
Year: 2024
Subjects: feminism
film analysis
gender
MeToo
pornography
sexuality
Communication

description: Situated in contemporary discourse where film critics and netizens alike debate the values of sex scenes and intimacy coordinators, this paper explores the impact of the MeToo movement on popular cinema. As a nearly endless stream of news stories about sexual assault continues to loom over the film industry, there is a heightened awareness of the use of women’s bodies and sexuality in films. This paper argues that the global feminist movement has informed storytellers’ (narrative, formal, aesthetic) choices. Reflecting the movement’s online component and a state of popular feminism (Banet-Weiser), audience judgements are brought in through discourse analysis, as well as close textual analyses of Worst Person in the World (2021, Norway) dir. Joachim Trier and Bad Luck Banging or Loony Porn (2021, Romania) dir. Radu Jude. The former mentions MeToo explicitly in the script, but both evoke the central tenets of the feminist movement by imagining what consent-based desire entails. The paper draws on contemporary feminist media scholars (Benson-Allott, Gilmore, Srinivasan) as well as feminist film theory (Mulvey) and porn studies (Williams and Young) to aid in its analyses. Trier’s film exemplifies an attempt to frame a woman’s sexual desire in a way that challenges masculine, objectifying camera gazes. Jude’s film provocatively uses amateur pornography to situate a woman’s sexual habits as an object of cultural debate. Together, the films represent MeToo’s international travel and influence on global film industries, gesturing toward a need for structural, feminist change in filmic representation.
Prevalence of Vitamin D Deficiency Varies Widely by Season in Canadian Children and Adolescents with Sickle Cell Disease
Creators: name: Samson, Kaitlyn L. I.
name: McCartney, Heather
name: Vercauteren, Suzanne M.
name: Wu, John K.
name: Karakochuk, Crystal D.
Publisher: name: Multidisciplinary Digital Publishing Institute
Year: 2019

description: Sickle cell disease (SCD) is an inherited disorder caused by a variant (rs334) in the β-globin gene encoding hemoglobin. Individuals with SCD are thought to be at risk of vitamin D deficiency. Our aim was to assess serum 25-hydroxyvitamin D (25OHD) concentrations, estimate deficiency prevalence, and investigate factors associated with 25OHD concentrations in children and adolescents with SCD attending BC Children’s Hospital in Vancouver, Canada. We conducted a retrospective chart review of SCD patients (2–19 y) from 2012 to 2017. Data were available for n = 45 patients with n = 142 25OHD measurements assessed using a EUROIMMUN analyzer (EUROIMMUN Medizinische Labordiagnostika AG, Lübeck, Germany). Additional data were recorded, including age, sex, and season of blood collection. Linear regression was used to measure associations between 25OHD concentration and predictor variables. Overall, mean ± SD 25OHD concentration was 79 ± 36 nmol/L; prevalence of low 25OHD concentrations (<30, <40, and <75 nmol/L) was 5%, 17% and 50%, respectively. Mean 25OHD concentrations measured during Jul–Sep were higher (28 (95% confidence interval CI: 16–40) nmol/L higher, P < 0.001) compared to Jan–Mar. Vitamin D deficiency rates varied widely by season: Based on 25OHD <30 nmol/L, prevalence was 0% in Oct–Dec and 6% in Jan–Mar; based on <40 nmol/L, prevalence was 0% in Oct–Dec and 26% in Jan–Mar.
Causes and consequences of gill remodelling in the goldfish, Carassius auratus
Creators: name: Tzaneva, Velislava
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2010
Subjects: subject: Health Sciences, Human Development.

description: Goldfish are able to drastically change their gill morphology by forming an interlamellar cell mass (ILCM) when acclimated to temperatures below 15 ° C. This morphological change is reversed when the fish is exposed to hypoxia or an increase in temperature. The main goal of this thesis was to investigate how the presence of the ILCM affects the transfer and sensing of respiratory gases as well as to identify the cues that trigger gill remodelling. Using an extracorporeal blood shunt to measure blood gases, I show that the presence of the ILCM does not impede O2 uptake under normoxic or hypoxic conditions but presents an additional diffusion limitation to CO 2 excretion owing to chemical equilibrium constraints. Hypoxemia induced by carbon monoxide or phenylhydrazine elicited a decrease in the ILCM in cold (70 C) acclimated goldfish while exposure to hyperoxia partially prevented the reduction of the ILCM during a temperature increase.
La rhétorique de laparodie dans le destin de Monique de Claire Bretécher.
Creators: name: Nguyen, Nhu-Hoa.
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2000
Subjects: subject: Literature, Romance.

description: Cette thèse étudie les mécanismes de la rhétorique parodique qu'utilise Claire Bretécher dans son album Le destin de Monique (1983), le seul album de ses oeuvres consacré entièrement à une unique diégèse, contrairement à sa pratique habituelle de gags d'une page ou d'une double-page. Une première section étudie les moyens dont est mise en place une rhétorique de la parodie textuelle. Recourant abondamment à la technique du récit-parenthèse, Bretécher met en scène des personnages dotés chacun d'un registre de langue particulier, issu non pas tellement d'une réalité linguistique que d'une composition créative de l'auteur. Une autre section analyse les procédés de rhétorique iconique et s'intéresse à la façon dont Bretécher exploite le langage visuel propre à la bande dessinée, soit les rapports entre planches, cases et dessins. Une attention particulière est apportée à la technique de la citation composite au moyen de laquelle sont détournées de façon iconoclaste de nombreuses peintures religieuses de la tradition classique. Á la lumière de ces analyses, it apparaît que Le destin de Monique fournit un bel exemple de transmission d'un message paralittéraire et humoristique, jouant habilement sur la connivence entre l'auteur et son lecteur. (Abstract shortened by UMI.)
3585244.pdf
Creators: name: Gao, Dingshan
name: Li, Shuyi
name: Zhou, Yangyang
name: Dong, Jianji
name: Zhang, Xinliang
name: Cassan, Eric
name: Hou, Jin
name: Yang, Chunyong
name: Chen, Shaoping
name: Chen, Huanyang
Publisher: name: Optica Publishing Group
Year: 2018
Subjects: Uncategorized

description: Supplement 1 for the article
Numerical calculations of ellipsoidal blast waves.
Creators: name: Mortimer, Mark F.
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 1990
Subjects: subject: Engineering, Mechanical.

description: This thesis investigates the progression of a blast wave created by the volumetric explosion of a pressurized ellipsoidal region. The use of numerical methods on a digital processing machine is made. Eight cases of four different ellipticities and two different overpressures were examined to determine the influence of the shape of the blast sources. It is shown that, for an ellipsoidal cold region generated from a volumetric explosion, the peak pressure through the intermediate fields would be up to three times greater than the peak pressure for an equivalent spherical region and occurs along the initially minor axis of the blast source. The static impulse and the dynamic impulse were also up to two to three times greater than those for an equivalent spherical region, through most of the intermediate and near far fields. A corresponding decrease in the values of these parameters was noted along the major axis.
Advisor: A system to develop and use online help.
Creators: name: Murphy, Mary Patricia.
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 1990
Subjects: subject: Computer Science.

description: The purpose of this thesis is to create a tool (Advisor) for the development and use of online help systems. The study of human-computer interface issues is central to this task. In particular, attention has been given to the analysis of learning principles as related to interface strategies. The Advisor software is used by help designers to develop help systems and by application users to get assistance during applications use. It has two parts: the Designer (editor/outliner used by the help developer for help screen definition) and the Browser (help viewer used by the software end user for assistance from within an application). Using hypertext technology, the Advisory creates a tutorial environment where help screens and keyword links provide context sensitive help to assist the application user. A simple interface exists for access to the Browser by an application program.
title: The elements of G
title: psychometric and neuroanatomical evidence that working memory is constitutive of general cognitive ability
titleType: Subtitle
Creators: name: Bowren, Mark
Publisher: name: University of Iowa
Year: 2022
Subjects: Brain Networks
General Cognitive Ability
General Intelligence
Lesion Method
Lesion-Behavior Mapping
Working Memory
public abstract
Clinical Psychology

description: General cognitive ability – or general intelligence (g) – is central to cognitive science, yet the processes that constitute it remain unknown, in good part because most prior work has relied on correlational methods. Large-scale behavioral and neuroanatomical data from neurological patients with focal brain lesions can be leveraged to advance our understanding of the key mechanisms of g, as this approach allows inference on the independence of cognitive processes along with elucidation of their respective neuroanatomical substrates. We analyzed behavioral and neuroanatomical data from 402 individuals (212 males; 190 females) with chronic, focal brain lesions. Structural equation models demonstrated a psychometric isomorphism between g and working memory in the present sample (which I refer to as g/Gwm), but not between g and other cognitive abilities. Multivariate lesion-behavior mapping analyses indicated that g and working memory localize most critically to a site of converging white matter tracts deep to the left temporo-parietal junction. Tractography analyses demonstrated that the regions in the lesion-behavior map of g were primarily associated with the arcuate fasciculus. The anatomical findings were validated in an independent cohort of acute stroke patients (n = 101) using model-based predictions of cognitive deficits generated from the Iowa cohort lesion-behavior maps. The neuroanatomical localization of g/Gwm provided the strongest prediction of observed g in the new cohort (r = 0.42, p < 0.001), supporting the anatomical specificity of my findings. These results provide converging behavioral and anatomical evidence that working memory is a key mechanism contributing to domain-general cognition.
title: Mismatch Repair-Deficient Crypt Foci in Lynch Syndrome
title: Molecular Alterations and Association with Clinical Parameters
titleType: Subtitle
Creators: name: Staffa, Laura
name: Echterdiek, Fabian
name: Nelius, Nina
name: Benner, Axel
name: Werft, Wiebke
name: Lahrmann, Bernd
name: Grabe, Niels
name: Schneider, Martin
name: Tariverdian, Mirjam
name: Knebel Doeberitz, Magnus Von
name: Bläker, Hendrik
name: Kloor, Matthias
Publisher: name: Charité - Universitätsmedizin Berlin
Year: 2015
Subjects: subject: 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit

description: Lynch syndrome is caused by germline mutations of DNA mismatch repair (MMR) genes, most frequently MLH1 and MSH2. Recently, MMR-deficient crypt foci (MMR- DCF) have been identified as a novel lesion which occurs at high frequency in the intestinal mucosa from Lynch syndrome mutation carriers, but very rarely progress to cancer. To shed light on molecular alterations and clinical associations of MMR-DCF, we systematically searched the intestinal mucosa from Lynch syndrome patients for MMR-DCF by immunohistochemistry. The identified lesions were characterised for alterations in microsatellite-bearing genes with proven or suspected role in malignant transformation. We demonstrate that the prevalence of MMR-DCF (mean 0.84 MMR-DCF per 1 cm2 mucosa in the colorectum of Lynch syndrome patients) was significantly associated with patients’ age, but not with patients’ gender. No MMR-DCF were detectable in the mucosa of patients with sporadic MSI-H colorectal cancer (n = 12). Microsatellite instability of at least one tested marker was detected in 89% of the MMR-DCF examined, indicating an immediate onset of microsatellite instability after MMR gene inactivation. Coding microsatellite mutations were most frequent in the genes HT001 (ASTE1) with 33%, followed by AIM2 (17%) and BAX (10%). Though MMR deficiency alone appears to be insufficient for malignant transformation, it leads to measurable microsatellite instability even in single MMR-deficient crypts. Our data indicate for the first time that the frequency of MMR-DCF increases with patients’ age. Similar patterns of coding microsatellite instability in MMR-DCF and MMR-deficient cancers suggest that certain combinations of coding microsatellite mutations, including mutations of the HT001, AIM2 and BAX gene, may contribute to the progression of MMR-deficient lesions into MMR-deficient cancers.
title: Opening time
title: stuttering, temporality, and blackness in There are no children here (1993)
titleType: Subtitle
Creators: name: Brubaker, Lydia
Publisher: name: University of Iowa
Year: 2024
Subjects: disability film
speech
stutter
stuttering

description: In the field of film studies, verbal disabilities like stuttering have often been overlooked beyond the observation that many films often use stuttering and characters who stutter as the punchline of a joke. This thesis focuses on moments of stuttering in Anita Addison's 1993 made-for-TV film There Are No Children Here and analyzes them through the lens of disability film theory, crip temporality, and Black temporality to imagine new temporalities and ways of holding time and space for disabilities on screen.
Hot-spot mix in ignition-scale inertial confinement fusion targets
Creators: name: Regan, S. P.
name: Epstein, R.
name: Hammel, B. A.
name: Suter, L. J.
name: Scott, H. A.
name: Barrios, M. A.
name: Bradley, D. K.
name: Callahan, D. A.
name: Cerjan, C.
name: Collins, G. W.
name: Dixit, S. N.
name: Döppner, T.
name: Edwards, M. J.
name: Farley, D. R.
name: Fournier, K. B.
name: Glenn, S.
name: Glenzer, S. H.
name: Golovkin, I. E.
name: Haan, S. W.
name: Hamza, A.
name: Hicks, Damien
name: Izumi, N.
name: Jones, O. S.
name: Kilkenny, J. D.
name: Kline, J. L.
name: Kyrala, G. A.
name: Landen, O. L.
name: Ma, T.
name: MacFarlane, J. J.
name: MacKinnon, A. J.
name: Mancini, R. C.
name: McCrory, R. L.
name: Meezan, N. B.
name: Meyerhofer, D. D.
name: Nikroo, A.
name: Park, H.-S.
name: Ralph, J.
name: Remington, B. A.
name: Sangster, T. C.
name: Smalyuk, V. A.
name: Springer, P. T.
name: Town, R. P. J.
Publisher: name: Swinburne
Year: 2024

description: Mixing of plastic ablator material, doped with Cu and Ge dopants, deep into the hot spot of ignition-scale inertial confinement fusion implosions by hydrodynamic instabilities is diagnosed with x-ray spectroscopy on the National Ignition Facility. The amount of hot-spot mix mass is determined from the absolute brightness of the emergent Cu and Ge K-shell emission. The Cu and Ge dopants placed at different radial locations in the plastic ablator show the ablation-front hydrodynamic instability is primarily responsible for hot-spot mix. Low neutron yields and hot-spot mix mass between 34(-13,+50) ng and 4000(-2970,+17 160) ng are observed.