Rhodocybe pakistanica Abdul & Khalid, 2024, sp. nov.
Creators: name: Abdul, Zaman Khan
name: Khalid, Abdul Nasir
Publisher: name: Zenodo
Year: 2024
Subjects: Biodiversity
Taxonomy
Fungi
Basidiomycota
Agaricomycetes
Agaricales
Entolomataceae
Rhodocybe
Rhodocybe pakistanica

description: Rhodocybe pakistanica sp. nov.
(Figs 3-5)
The species is mainly characterized by centrally depressed and dull reddish brown or dull orange pileus with bright patches, light yellow to orange lamellae, light reddish gray and fibrillose stipe, ovoid or oblong basidiospores, lanceolate, subulate or ventricose-rostrate cheilo and pleurocystidia, and presence of gloeoplerous hyphae in pileal and hymenial trama.
TYPE MATERIAL. — Pakistan • Punjab Province, District Khushab, Noorpur Thal, Peelowains, on moist sandy soil with litter; 32°01’22.4”N, 71°59’32.4”E; 191 m a.s.l.; 01.VIII.2022; Zaman Khan & Junaid Khan P-154; holotype: LAH37947; GenBank: OR606543 (ITS), OR606541 (28S).
ADDITIONAL MATERIAL EXAMINED. — Pakistan • Punjab Province, District Khushab, Noorpur Thal, Peelowains, on moist sandy soil near Ziziphus nummularia (Burm.f.) Wight & Arn.; 31°59’17.36”N, 71°59’25.86”E; 191 m a.s.l.; 30.VII.2022; Zaman Khan & Junaid Khan P-146; LAH37948; GenBank: OR606544 (ITS), OR606542 (28S) • ibid.; 02.VIII.2022; Zaman Khan & Junaid Khan P-171; LAH37949; GenBank: OR606545 (ITS).
ETYMOLOGY. — The specific epithet ‘pakistanica’ refers to the type locality of species.
HABIT, HABITAT, AND DISTRIBUTION. — Saprotrophic, solitary, terrestrial, on moist, sandy soil with litter in forest, near Dalbergia sissoo, and Ziziphus nummularia. Presently only known from Punjab, Pakistan.
MYCOBANK. — MB850258.
DESCRIPTION
Basidiomata
18-30 mm in length, small-sized. Pileus 7-28 mm diam., surface dull reddish brown (5YR 4/4, 5/4), at center becoming dull orange (7.5YR 6/4) or pale (5YR 8/3) towards periphery, with bright patches (7.5YR 5/6) scattering towards margins when mature, initially convex or hemispherical, depressed at center, becoming applanate or planoconvex and slightly depressed with age, margins decurved or plane at initial stage, uplifted or flaring upon maturity, undulate, entire.
Lamellae
Light yellow (10YR 8/3) to orange (10YR 8/4) or light (5YR 8/1) to gray (5YR 8/2), subdecurrent to decurrent, arcuate, broad or ventricose, brittle, entire or undate margins, lamellulae present in four tiers.
Stipe
15-28 × 2-4 mm, central, cylindrical or slightly tapering downwards, dull (2.5YR 6/3) to pale reddish (2.5 YR 7/3) or light reddish gray (7.5R 7/1) to grayish red (7.5R 6/2), with whitish fibrils on the surface, with rhizomorphs and whitish mycelial threads.
Context
Solid, pale reddish. Odor and taste not recorded.
Basidiospores
[50/3/3], 5.3-7×3.8-4.6 µm, avl×avw=5.9×4.2 µm, Q=1.35- 1.52, avQ=1.42, ovoid or oblong, sometimes ellipsoid, slightly undulate-pustulate, cyanophilic, inamyloid, non-dextrinoid, with prominent apiculus.
Basidia
21-26 ×6-8 µm, narrowly clavate to clavate, cyanophilic, with refractive contents in 5% KOH, thin-walled, two to four spored, sterigmata up to 4.4 µm in length.
0.07
Cheilo- and pleurocystidia
Rarely present, 24-37 × 4-7 µm, lanceolate, subulate, ventricose-rostrate, sometimes fusiform, cylindrical or clavate, with golden ochre or pale yellowish refractive contents in 5% KOH.
Lamellar trama
Consisting of 5.5-12.5 µm broad, regular, cylindrical, interwoven hyphae, hyaline in 5% KOH.
Gloeoplerous hyphae in hymenial and pileal trama
3.2-7.7 µm broad, cylindrical, elongated, with narrow endings, incrusted, golden refringent in 5% KOH.
Pileipellis
A cutis, consisting of 2.4-6.7 µm broad, cylindrical or elongate, thin-walled hyphae, brownish in 5% KOH.
Stipitipellis
A cutis, consisting of 3.2-8.8 µm broad, cylindrical, parallel, rarely branched hyphae, with clavate or narrowly fusiform ending elements, hyaline in 5% KOH.
Caulocystidia
Absent.
Clamp connections
Absent in all tissues.
description: Published as part of Abdul, Zaman Khan & Khalid, Abdul Nasir, 2024, Molecular phylogeny and morphology reveal a new species of genus Rhodocybe sensu stricto Maire (Entolomataceae; Agaricales) from Pakistan, pp. 127-137 in Cryptogamie, Mycologie 20 (10) on pages 129-131, DOI: 10.5252/cryptogamie-mycologie2024v45a10, http://zenodo.org/record/13891884