title: ÐÐ»Ð»ÐµÐ»ÑÐ½ÑÐµ Ð²Ð°ÑÐ¸Ð°Ð½ÑÑ Ð¸ ÑÑÐ¾Ð²ÐµÐ½Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð° scarb2 Ð² Ð¿Ð°ÑÐ¾Ð³ÐµÐ½ÐµÐ·Ðµ Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ Ð¿Ð°ÑÐºÐ¸Ð½ÑÐ¾Ð½Ð°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Allelic variants and gene expression level SCARB2 in the pathogenesis of Parkinson's disease
Creators: name: ÐÐ¾Ð³Ð´Ð°Ð½Ð¾Ð²Ð°, ÐÐ°ÑÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: Ð±Ð¾Ð»ÐµÐ·Ð½Ñ ÐÐ°ÑÐºÐ¸Ð½ÑÐ¾Ð½Ð°
scarb2
Ð»Ð¸Ð·Ð¾ÑÐ¾Ð¼Ñ
Ð¾Ð´Ð½Ð¾Ð½ÑÐºÐ»ÐµÐ¾ÑÐ¸Ð´Ð½ÑÐ¹ Ð¿Ð¾Ð»Ð¸Ð¼Ð¾ÑÑÐ¸Ð·Ð¼
Parkinson's disease
lysosomes
single nucleotide polymorphism

description: ÐÐ°ÐºÐ°Ð»Ð°Ð²ÑÑÐºÐ°Ñ ÑÐ°Ð±Ð¾ÑÐ° Â«ÐÐ»Ð»ÐµÐ»ÑÐ½ÑÐµ Ð²Ð°ÑÐ¸Ð°Ð½ÑÑ Ð¸ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð³ÐµÐ½Ð° SCARB2 Ð² Ð¿Ð°ÑÐ¾Ð³ÐµÐ½ÐµÐ·Ðµ Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ ÐÐ°ÑÐºÐ¸Ð½ÑÐ¾Ð½Ð°Â» Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¾ÑÐµÐ½ÐºÐµ Ð°ÑÑÐ¾ÑÐ¸Ð°ÑÐ¸Ð¹ Ð¾Ð´Ð½Ð¾Ð½ÑÐºÐ»ÐµÐ¾ÑÐ¸Ð´Ð½ÑÑ Ð¿Ð¾Ð»Ð¸Ð¼Ð¾ÑÑÐ¸Ð·Ð¼Ð¾Ð² (ÐÐÐ) rs6812193 Ð¸ rs8475 Ð³ÐµÐ½Ð° SCARB2 Ñ ÑÐ¸ÑÐºÐ¾Ð¼ Ð¸ Ð²Ð¾Ð·ÑÐ°ÑÑÐ¾Ð¼ Ð½Ð°ÑÐ°Ð»Ð° Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ ÐÐ°ÑÐºÐ¸Ð½ÑÐ¾Ð½Ð° (ÐÐ) Ð² Ð¡ÐµÐ²ÐµÑÐ¾-ÐÐ°Ð¿Ð°Ð´Ð½Ð¾Ð¼ ÑÐµÐ³Ð¸Ð¾Ð½Ðµ Ð Ð¾ÑÑÐ¸Ð¸. Ð ÑÐ¾Ð´Ðµ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ» ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾-Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð¼ÐµÑÐ¾Ð´ Ð¸Ð´ÐµÐ½ÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ ÐÐÐ rs6812193 Ð¸ rs8475 Ð³ÐµÐ½Ð° SCARB2, Ð¾ÑÑÑÐµÑÑÐ²Ð»ÐµÐ½ ÑÐºÑÐ¸Ð½Ð¸Ð½Ð³ Ð¾Ð´Ð½Ð¾Ð½ÑÐºÐ»ÐµÐ¾ÑÐ¸Ð´Ð½ÑÑ Ð¿Ð¾Ð»Ð¸Ð¼Ð¾ÑÑÐ¸Ð·Ð¼Ð¾Ð² (ÐÐÐ) rs6812193 Ð¸ rs8475 Ð³ÐµÐ½Ð° SCARB2, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¾ÑÐµÐ½ÐµÐ½Ð° ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð³ÐµÐ½Ð° SCARB2 Ð² Ð³ÑÑÐ¿Ð¿Ðµ Ð¿Ð°ÑÐ¸ÐµÐ½ÑÐ¾Ð² Ñ ÐÐ Ð¸ Ð² ÑÐ°ÐºÐ¶Ðµ Ð² ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½Ð¾Ð¹ Ð³ÑÑÐ¿Ð¿Ðµ (Ð³ÑÑÐ¿Ð¿Ð° Ð¸Ð½Ð´Ð¸Ð²Ð¸Ð´ÑÑÐ¼Ð¾Ð² Ð±ÐµÐ· Ð½ÐµÐ²ÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ð¹). ÐÐ°Ð½Ð½Ð¾Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð²Ð¿ÐµÑÐ²ÑÐµ Ð²ÑÑÐ²Ð¸Ð»Ð¾ ÑÐ²ÑÐ·Ñ rs8475 SCARB2 Ñ ÐÐ: Ð²Ð°ÑÐ¸Ð°Ð½Ñ TT rs8475 Ð³ÐµÐ½Ð° SCARB2 ÑÐ²ÐµÐ»Ð¸ÑÐ¸Ð²Ð°ÐµÑ ÑÐ¸ÑÐº ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ñ ÐÐ Ð² Ð´Ð²Ð° ÑÐ°Ð·Ð°. rs6812193 Ð½Ðµ Ð°ÑÑÐ¾ÑÐ¸Ð¸ÑÐ¾Ð²Ð°Ð½ Ñ ÑÐ¸ÑÐºÐ¾Ð¼ ÐÐ. rs6812193 Ð¸ rs8475 Ð³ÐµÐ½Ð° SCARB2 Ð½Ðµ ÑÐ²Ð»ÑÑÑÑÑ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ð¼ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¾ÑÐ¾Ð¼ Ð²Ð¾Ð·ÑÐ°ÑÑÐ° Ð½Ð°ÑÐ°Ð»Ð° ÐÐ Ð¸ Ð½Ðµ Ð²Ð»Ð¸ÑÑÑ Ð½Ð° ÑÑÐ¾Ð²ÐµÐ½Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ SCARB2 Ð² CD45 + ÐºÐ»ÐµÑÐºÐ°Ñ ÐºÑÐ¾Ð²Ð¸. ÐÑÐ¸ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð¸Ð¸ Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÑÐºÐ¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¸ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ñ Ð¿ÑÐ°Ð¹Ð¼ÐµÑÑ Ð¸ Ð·Ð¾Ð½Ð´Ñ Ð´Ð»Ñ ÐÐ¦Ð  Ð¸ ÐÐ¦Ð  Ð² ÑÐµÐ¶Ð¸Ð¼Ðµ ÑÐµÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð²ÑÐµÐ¼ÐµÐ½Ð¸ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼ Vector NTI Ð¸ Primer3. ÐÑÐ¾Ð²ÐµÐ´ÐµÐ½Ñ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÑ: Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð½Ð°Ñ ÑÐµÐ¿Ð½Ð°Ñ ÑÐµÐ°ÐºÑÐ¸Ñ (ÐÐ¦Ð ) Ñ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐ¸Ð¼ ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¾Ð½Ð½ÑÐ¼ Ð°Ð½Ð°Ð»Ð¸Ð·Ð¾Ð¼, ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÐ· ÑÑÐ°Ð³Ð¼ÐµÐ½ÑÐ¾Ð² Ð² Ð¿Ð¾Ð»Ð¸Ð°ÐºÑÐ¸Ð»Ð°Ð¼Ð¸Ð´Ð½Ð¾Ð¼ Ð³ÐµÐ»Ðµ, Ð°Ð»Ð»ÐµÐ»Ñ-ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐ½Ð°Ñ ÐÐ¦Ð  Ð² ÑÐµÐ¶Ð¸Ð¼Ðµ ÑÐµÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð²ÑÐµÐ¼ÐµÐ½Ð¸, ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²ÐµÐ½Ð½Ð°Ñ ÐÐ¦Ð  Ð² ÑÐµÐ¶Ð¸Ð¼Ðµ ÑÐµÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð²ÑÐµÐ¼ÐµÐ½Ð¸, ÑÐµÐºÐ²ÐµÐ½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ, ÑÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÐ°Ñ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÐ° Ð´Ð°Ð½Ð½ÑÑ.
description: Bachelor's work âAllelic variants and expression of the SCARB2 gene in the pathogenesis of Parkinson's diseaseâ is devoted to assessing of the associations of single nucleotide polymorphisms (SNPs) rs6812193 and rs8475 of the SCARB2 gene with risk and age at onset of Parkinson's disease (PD) in the North-West region of Russia. In the current work method for identifying of SNPs rs6812193 and rs8475 of the SCARB2 gene was designed. rs6812193 and rs8475 of the SCARB2 gene were genotyped in the group of patients with PD and controls. The current study revealed for the first time association rs8475 of SCARB2 with PD: TT variant of rs8475 of the SCARB2 gene increase twice risk of PD. No association of rs6812193 with PD risk was found for Russian population. rs6812193 and rs8475 polymorphisms of SCARB2 are not genetic modifier of age at onset of PD and do not influence on SCARB2 expression level in CD45+ blood cells. In thus bachelor's work sequences of primers for genotyping of studied SNPs and estimating of SCARB2 mRNA level were designed by Vector NTI and Primer3 programs. The following experiments were carried out: polymerase chain reaction (PCR)ârestriction fragment length polymorphism method, allele-specific real-time PCR, real-time PCR, direct sequencing of DNA sample with forward and reverse primers, and statistical analysis.