Insights into the origin of metazoan multicellularity from predatory unicellular relatives of animals
Creators: name: Tikhonenkov, Denis
name: Hehenberger, Elisabeth
name: Esaulov, Anton S.
name: Belyakova, Olga I.
name: Mazei, Yuri A
name: Mylnikov, Alexander P.
name: Keeling, Patrick J.
Publisher: name: figshare
Year: 2020
Subjects: subject: Evolutionary Biology
subject: FOS: Biological sciences
subject: FOS: Biological sciences

description: 1) Videos of morphology, life cycle, and feeding of novel predatory unicellular relatives of animals for "Insights into the origin of metazoan multicellularity from predatory unicellular relatives of animals"Video 1. Swimming of <i>Syssomonas multiformis </i>cell with rotation. Video 2. Attached cell of <i>Syssomonas multiformis </i>and rapid flagellum beating. Video 3. Amoeboflagellate stage of <i>Syssomonas multiformis. </i>Cells of eukaryotic prey <i>Parabodo caudatus</i> are also visible. Video 4. Loss of flagellum in <i>Syssomonas multiformis</i> and transition to amoeba. Video 5. Transformation of amoeba into a cyst in <i>Syssomonas multiformis</i>. Video 6. Palintomic divisions inside the cyst of <i>Syssomonas multiformis</i>. Video 7. Division into two cell structures in <i>Syssomonas multiformis.</i> Video 8. Cell and cyst of <i>Syssomonas multiformis</i> with vesicular structures inside. Video 9. Feeding of <i>Syssomonas multiformis </i>on eukaryotic prey<i>.</i> Video 10. Feeding of <i>Syssomonas multiformis </i>on bacteria. Video 11. Temporary cell aggregations of <i>Syssomonas multiformis.</i> Video 12. Floating rosette-like aggregation of <i>Syssomonas multiformis.</i> Video 13. Syncytium-like structures and budding of young flagellated daughter cells in <i>Syssomonas multiformis.</i> Video 14. Joint feeding of <i>Pigoraptor vietnamica </i>on dead cell of <i>Parabodo caudatus.</i> Video 15. Joint feeding of <i>Pigoraptor chileana </i>on dead cell of <i>Parabodo caudatus.</i> Video 16. Temporary cell aggregation of <i>Pigoraptor chileana.</i><b></b><br>2) Tree files (as newick and pdf files) and corresponding alignments for "Insights into the origin of metazoan multicellularity from predatory unicellular relatives of animals"<br>"alpha_amylase_1_LG_I_G4_1000UFboot_newick.tre": raw tree file in newick format of alpha amylase 1, corresponding to Table 1. Tree reconstruction performed with IQ-TREE (selected model based on ModelFinder: LG+I+G4, branch support was assessed with 1000 ultrafast bootstrap replicates).<br>"alpha_amylase_1_LG_I_G4_1000UFboot.tre.pdf": unrooted tree of alpha amylase 1 as pdf file, corresponding to Table 1. Colored by lineage.<br>"alpha_amylase_1_trimal0.8.fasta": trimmed alignment used for tree reconstruction of alpha amylase 1<br>"alpha_amylases_2_3_4_5_LG_R10_1000UFboot_newick.tre": raw tree file in newick format of alpha amylases 2-5, corresponding to Table 1. Tree reconstruction performed with IQ-TREE (selected model based on ModelFinder: LG+R10, branch support was assessed with 1000 ultrafast bootstrap replicates).<br>"alpha_amylases_2_3_4_5_LG_R10_1000UFboot.tre.pdf": unrooted tree of alpha amylases 2-5 as pdf file, corresponding to Table 1. Colored by lineage.<br>"alpha_amylases_2_3_4_5_trimal0.8.fasta": trimmed alignment used for tree reconstruction of alpha amylases 2-5<br>"alpha_glucosidases_1_2_3_4_LG_R10_1000UFboot_newick.tre": raw tree file in newick format of alpha glucosidases 1-4, corresponding to Table 1. Tree reconstruction performed with IQ-TREE (selected model based on ModelFinder: LG+R10, branch support was assessed with 1000 ultrafast bootstrap replicates).<br>"alpha_glucosidases_1_2_3_4_LG_R10_1000UFboot.tre.pdf": unrooted tree of alpha glucosidases 1-4 as pdf file, corresponding to Table 1. Colored by lineage.<br>"alpha_glucosidases_1_2_3_4_trimal0.8.fasta": trimmed alignment used for tree reconstruction of alpha glucosidases 1-4<br>"glycogen_debranching_enzyme_AGL_LG_R7_1000UFboot_newick.tre": raw tree file in newick format of glycogen debranching enzyme AGL, corresponding to Table 1. Tree reconstruction performed with IQ-TREE (selected model based on ModelFinder: LG+R7, branch support was assessed with 1000 ultrafast bootstrap replicates).<br>"glycogen_debranching_enzyme_AGL_LG_R7_1000UFboot.tre.pdf": unrooted tree of glycogen debranching enzyme AGL as pdf file, corresponding to Table 1. Colored by lineage.<br>"glycogen_debranching_enzyme_AGL_trimal0.8.fasta": trimmed alignment used for tree reconstruction of glycogen debranching enzyme AGL<br>"glycogen_phosphorylase_PYG_LG_R10_1000UFboot_newick.tre": raw tree file in newick format of glycogen phosphorylase PYG, corresponding to Table 1. Tree reconstruction performed with IQ-TREE (selected model based on ModelFinder: LG+R10, branch support was assessed with 1000 ultrafast bootstrap replicates).<br>"glycogen_phosphorylase_PYG_LG_R10_1000UFboot.tre.pdf": unrooted tree of glycogen phosphorylase PYG as pdf file, corresponding to Table 1. Colored by lineage.<br>"glycogen_phosphorylase_PYG_trimal0.8.fasta": trimmed alignment used for tree reconstruction of glycogen phosphorylase PYG