title: ÐÐ·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ðµ ÐÐ¢Ð¤ Ñ Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð½ÑÐ¼Ð¸ ÑÐ¸Ð±ÑÐ¸Ð»Ð»Ð°Ð¼Ð¸ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ Ð»Ð¸Ð·Ð¾ÑÐ¸Ð¼Ð°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: The interaction between ATP and lysozyme-based amyloid fibrils
Creators: name: ÐÑÐ¸Ð´Ð°ÑÐ¾Ð²Ð°, ÐÑÐ¸ÑÑÐ¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð½ÑÐµ ÑÐ¸Ð±ÑÐ¸Ð»Ð»Ñ
ÐÐ¢Ð¤
Ð»Ð¸Ð·Ð¾ÑÐ¸Ð¼
Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð¾Ð·
ÑÐ°Ð²Ð½Ð¾Ð²ÐµÑÐ½ÑÐ¹ Ð¼Ð¸ÐºÑÐ¾Ð´Ð¸Ð°Ð»Ð¸Ð·
ÐºÑÑÐ³Ð¾Ð²Ð¾Ð¹ Ð´Ð¸Ñ ÑÐ¾Ð¸Ð·Ð¼
ÐºÑÐ¸Ð²ÑÐµ Ð¡ÐºÑÑÑÐ°ÑÐ´Ð°
amyloid fibrils
ATP
lysozyme
amyloidosis
equilibrium microdialysis
circular dichroism
Scatchard curves

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description: The accumulation of amyloid fibrils is a marker of serious diseases. Cellular aging, along with amyloidogenesis, results in a decrease in ATP synthesis. It is known that ATP can stabilize the protein and directly inhibit its aggregation. At the same time, it has been poorly studied whether ATP is able to bind to mature amyloid fibrils and what effect it has on them. Therefore, the purpose of this work is to study the interaction between ATP and lysozyme-based amyloid fibrils. Methods of the work: equilibrium microdialysis, electron microscopy, circular dichroism method, electrophoresis, Scatchard curves. Results: lysozyme amyloidogenesis was initiated by protein exposure to a denaturing agent at elevated temperature. By electron microscopy, far-UV circular dichroism and pseudo-native SDS-PAGE, it was shown that amyloid fibrils with a high degree of clustering were obtained. The degree of clustering of lysozyme amyloid fibrils decreased as a result of the ATP exposure, as confirmed by electron microscopy and a decrease in the turbidity of the studied samples. Based on changes in optical activity in the near-UV region of the fibrils and analysis of Scatchard curves, it was concluded that lysozyme amyloid fibrils interact with ATP. The results of this study can be used by scientists developing approaches to the treatment of amyloidosis and neurodegenerative diseases.