cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Phenotypic and molecular characterization of Spe-43, a gene required for Caenorhabditis elegans spermiogenesis
Creators: name: Méndez, Ernesto R.
Publisher: name: No Publisher Supplied
Year: 2013

description: The reproduction of a genetically distinct new organism is a delicate and tightly regulated process. It requires a lot of molecular interactions and time-coordinated events. Before the fertilization process takes place, both male and female gametes need to properly develop and be fully capable to find one another, fuse, and promote the transition into a developing embryo. Different types of research performed in Caenorhabtidis elegans and Ascaris suum have helped provide a fairly clear picture of the cellular components and processes necessary for sperm development and differentiation. Still, some of the molecular mechanisms for these processes collectively known as spermatogenesis and spermiogenesis, are not fully understood. For those purposes, this thesis elucidates a new gene member required for C. elegans spermiogenesis: spe-43(eb63).   The first chapter of this thesis provides an introduction to the importance of C. elegans fertility research and its impact on modern society reproductive technologies. Also in this chapter we will review some basic knowledge about the C. elegans reproductive world such as gender, reproductive tract, gamete development, nematode sperm, spermatogenesis, spermiogenesis and fertilization.   The second chapter of the present work outlines the phenotypic and partial molecular characterization of the spe-43 gene. This new gene belongs to a family of genes called the spe-8 group, which they all interact in a specific spermiogenesis pathway of the C. elegans. This spermiogenesis process is not only critical for sperm development and fertilization in C. elegans, but represents a unique and important model both for the study of differentiation without de novo protein synthesis, cell maturation, cell mobility and for the evolutionary development of an androdioecious species from a gonochoristic one.
New biologic treatments for human pancreatic cancer
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Pancreatic carcinoma is the fourth leading cause of cancer death in men and women in the US. The overall 5-year survival rate is less than 5% and effective therapies are largely lacking, as most patients present with advanced disease. New strategies to manage this cancer are urgently needed. Metformin (1,1-dimethyl-bi-guanide hydrochloride) is a widely-used antihyperglycemic drug for diabetes mellitus type 2, and is now reported to have antitumor efficacy in pancreatic cancer. It’s primary systemic effect is to lower blood glucose, but metformin also reduces hyperinsulinemia in insulin resistance. At a cellular level, metformin stimulates AMP-activated protein kinase (AMPK) activation. Activation of AMPK inhibits mTORC1 which integrates signals from a diverse array of cell pathways to regulate pancreatic cancer progression. Thus, it is postulated that metformin inhibits pancreatic cancer in part via AMPK-mediated inhibition of mTORC1 activation. Other data show that metformin also disrupts critical crosstalk between insulin/IGF-1 and GPCR signaling pathways and possibly ERK. Laboratory studies show that metformin markedly inhibits growth of human pancreatic cancer cell xenografts in vivo. These preclinical data are supported by clinical cohort studies showing that metformin users have a reduced risk of pancreatic cancer; and that metformin use correlates with a survival benefit in patients with diabetes and pancreatic cancer. In the latter study, the median survival is only prolonged by 4 months in cancer patients who are metformin users compared to non-users. Thus, discovery of more potent anticancer analogs of metformin may boost clinical benefit. We plan to design and prepare metformin analogs never before synthesized and test their antitumor activity using pancreatic cancer cell models. We hypothesize that congeners of metformin can be synthesized with enhanced anticancer activity and minimal toxicity to non-target tissues. We will assess antitumor activity of metformin analogs in vitro using established assays for cell proliferation, apoptosis and migration/invasion. Cell signaling related to antitumor activity of metformin and analogs (such as AMPK, mTOR, insulin signaling) will be explored. Nonmalignant human cells will be used to assess effects on normal cells. A promising metformin analog will be selected for use with human pancreatic tumor xenografts in nude mouse to assess antitumor effects. A drug candidate for in vivo study will be selected on the basis of in vitro experiments to assess opt
Odontognathus compressus Meek & Hildebrand 1923
Creators: name: Angulo, Arturo
name: Betts, Joel T.
name: González-Alemán, Néstor J.
name: Castañeda, Edgar
name: Berghe, Eric Van Den
name: Elías, Diego J.
name: Mcmahan, Caleb D.
name: Matamoros, Wilfredo A.
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Chordata
Clupeiformes
Pristigasteridae
Odontognathus
Odontognathus compressus

description: Odontognathus compressus Meek & Hildebrand 1923 (Fig. 6J). Caribbean Longfin Herring; Arenquillo Machete
Vouchers: Not available. Distribution: Western Atlantic Ocean; from southern Nicaragua to Suriname; entering estuaries and littoral lagoons and ascending river courses; (Nicaragua) BF (Atlantic); 0–5 masl; Per, Dia, Amp. Occurrence and conservation status: Nat; LC (2017), population trend unknown. Literature: van den Berghe (2015: 44, illustrated but not identified; listed, including an illustration and information on distribution and ecology) and Robertson & Van Tassell (2019: 2929; detailed description, including illustrations, and information on distribution, with a map, and ecology). Remarks: Robertson & Van Tassell (2019) report a distribution for the species that ranges from the central coast of Costa Rica to Suriname; the present record, based on the above, would represent an extension in the known geographic range of the species of about 100 km to the north.
description: Published as part of Angulo, Arturo, Betts, Joel T., González-Alemán, Néstor J., Castañeda, Edgar, Berghe, Eric Van Den, Elías, Diego J., Mcmahan, Caleb D. & Matamoros, Wilfredo A., 2023, Continental fishes of Nicaragua: diversity, distribution and conservation status; with an annotated and illustrated checklist of species and an identification guide to families, pp. 1-89 in Zootaxa 5376 (1) on page 18, DOI: 10.11646/zootaxa.5376.1.1, http://zenodo.org/record/10208788
EKOLOGIK OMILLARNING YURAK-QON TOMIR TIZIMIGA SALBIY TA'SIRI: ZAMONAVIY QARASHLAR
Creators: name: Abdullaev, Nodirbek
Publisher: name: Zenodo
Year: 2025

description: Hozirgi zamonning eng dolzarb muammolaridan biri atrof-muhit o'zgarishlari va ularning inson salomatligiga ta'siridir. Iqlim o'zgarishi, havoning ifloslanishi, tabiiy resurslarning iste'moli va stress darajasining oshishi ko'plab kasalliklarning rivojlanishiga sabab bo'lmoqda. Ayniqsa, yurak-qon tomir kasalliklari (YQTK) insonlar orasida yuqori darajada tarqalmoqda.
Pedaridium martinezi Ferreira & Galileo 1993
Creators: name: Groll, Elisa Von
Publisher: name: Zenodo
Year: 2017
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Coleoptera
Scarabaeidae
Pedaridium
Pedaridium martinezi

description: Pedaridium martinezi Ferreira & Galileo, 1993 [current name: Martinezidium fulgens (Arrow, 1913)]
Ferreira & Galileo, 1993:30, figs 174–183, 238; Vaz-de-Mello, 2008:35, fig. 24 (syn).
Paratype: 1 specimen, ARGENTINA, Santiago del Estero, 25.XI.1953, R. Costas col. (MCNZ 234.842).
description: Published as part of Groll, Elisa Von, 2017, Catalogue of Coleoptera type specimens housed in the collection of the Museu de Ciências Naturais, Fundação Zoobotânica do Rio Grande do Sul, Brazil, pp. 439-473 in Zootaxa 4318 (3) on page 465, DOI: 10.11646/zootaxa.4318.3.2, http://zenodo.org/record/887750
The stressed female brain
Creators: name: Maeng, Lisa Y.
Publisher: name: No Publisher Supplied
Year: 2013

description: Women are nearly twice as likely as men to suffer from depression, which may be the result of a greater vulnerability to stress in females (Tolin & Foa, 2006).  A profound sex difference in the response to stress is also observed in laboratory animals.  Acute stress exposure disrupts associative learning in female rats but enhances learning in male rats (Wood & Shors, 1998).  These sex differences in response to stress are mediated by different brain regions.  For example, neuronal activity in the medial prefrontal cortex (mPFC) during the stressor is necessary to modify learning in females but not to modify learning in males (Maeng et al., 2010).  The medial prefrontal cortex can be divided into different subregions: the prelimbic (PL) and the infralimbic (IL).  There are structural and functional differences between the two areas.  For instance, the prelimbic cortex projects more heavily to limbic structures such as the basolateral amygdala; in contrast, the infralimbic cortex projects more to sites involved in visceromotor processes (Vertes, 2004).  Because the stress effect on learning in females relies on communication between the mPFC and the basolateral amygdala (Maeng et al., 2010), it was hypothesized that neural activity within the PL during the stressor would be necessary in order to suppress learning, whereas neural activity within the IL would not be necessary.  To test this hypothesis, two separate experiments were conducted.  In the first experiment, the prelimbic subregion of the mPFC in adult female rats was bilaterally inactivated with GABAA agonist muscimol.  In the second experiment, the infralimbic area of the medial prefrontal cortex was bilaterally inactivated with muscimol.  The animals were then exposed to acute inescapable swim stress or left unstressed.  One day later, all subjects were trained with classical conditioning of the eyelid response, using a white noise conditioned stimulus paired with an eyelid stimulation unconditioned stimulus.  They were trained with 100 paired trials a day for four consecutive days.  Interestingly, females without neuronal activity in the PL during the stressor were able to learn well.  The percentage of learned responses was significantly different from that expressed by females in which IL activity was suppressed; these females did not learn well after the stressor.  Together, these data suggest that stress exposure critically engages the prelimbic, but not infralimbic, subregion of the mPFC to suppress learning in females.  Moreover, because the suppressed learning after stress depends on communication between the mPFC and the amygdala, this communication must be via the prelimbic region.  Together, these data suggest that neuronal communication between the prelimbic cortex and the amygdala mediates the enhanced vulnerability to stress in females.  This circuit may be especially responsive in women who develop depression triggered by stressful life events.
Additional file 1: Table S1. of The protective role of religiosity against problem gambling: findings from a five-year prospective study
Creators: name: Mutti-Packer, Seema
name: Hodgins, David
name: Williams, Robert
name: Thege, Barna Konkolÿ
Publisher: name: Figshare
Year: 2017
Subjects: subject: Sociology
subject: FOS: Sociology
subject: Developmental Biology
subject: Science Policy

description: Contrasts between all categories of religious affiliation when examining the influence of frequency of religious service attendance on the intercept and slope of problem gambling severity, by gender (n = 3959). Table presenting all contrasts between religious affiliation. (DOCX 14 kb)
Dorosomatidae Gill 1861
Creators: name: Angulo, Arturo
name: Betts, Joel T.
name: González-Alemán, Néstor J.
name: Castañeda, Edgar
name: Berghe, Eric Van Den
name: Elías, Diego J.
name: Mcmahan, Caleb D.
name: Matamoros, Wilfredo A.
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Chordata
Clupeiformes
Dorosomatidae

description: Family Dorosomatidae Gill 1861 (Fig. 2L). Thread Herrings, Gizzard Shads, Sardinellas; Sardinas, Arenques, Sabaletes
Description: Body slightly elongate, fusiform, oval in cross-section; usually up to 20 cm in length; mouth usually inferior, short, ending before eye; upper jaw with a distinct median notch or cleft lower jaw flared outward; teeth absent; abdominal scutes strong; last dorsal-fin ray filamentous in most taxa; anal fin usually with 12–29, up to 38 in some taxa; usually silvery in color (Robertson & Allen 2015, Nelson et al. 2016). Distribution: Marine or freshwater; North and Central America, Indo-West Pacific Ocean (Nelson et al. 2016). Four genera and five species in Nicaraguan freshwaters.
description: Published as part of Angulo, Arturo, Betts, Joel T., González-Alemán, Néstor J., Castañeda, Edgar, Berghe, Eric Van Den, Elías, Diego J., Mcmahan, Caleb D. & Matamoros, Wilfredo A., 2023, Continental fishes of Nicaragua: diversity, distribution and conservation status; with an annotated and illustrated checklist of species and an identification guide to families, pp. 1-89 in Zootaxa 5376 (1) on page 19, DOI: 10.11646/zootaxa.5376.1.1, http://zenodo.org/record/10208788
Gene-environment interactions in transplacental ovarian carcinogenesis
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: It is increasingly appreciated that interactions between environmental exposures and genetic factors are important in carcinogenesis and that the in utero environment modifies the risk for developing diseases, including cancer, in adulthood. We propose the innovative hypothesis that embryonic and fetal glutathione (GSH) status modifies transplacental ovarian carcinogenesis. GSH, the most abundant intracellular thiol, acts as a cofactor in the glutathione transferase-mediated Phase II biotransformation of xenobiotic carcinogens, such as polycyclic aromatic hydrocarbons (PAHs). As a critical cellular antioxidant, GSH also detoxifies reactive oxygen species that are produced as a result of xenobiotic metabolism. Mutations or polymorphisms in the genes encoding the enzymes of GSH synthesis, in particular, glutamate cysteine ligase modifier (Gclm) and catalytic (Gclc) subunits, may enhance transplacental ovarian carcinogenesis by xenobiotic carcinogens, and supplementation of GSH or other antioxidants during pregnancy may prevent transplacental ovarian carcinogenesis. These studies will be the first to investigate the role of Gclm in carcinogenesis. If successful, they will enable primary prevention of ovarian cancer via efforts to decrease exposure to PAHs and will offer the possibility of chemoprevention of ovarian cancer. Specific Aim 1, Effect of Gclm deletion on PAH-induced transplacental ovarian tumorigenesis: Our preliminary data are consistent with an increased rate of ovarian tumorigenesis in response to in utero treatment with the PAH benzo[a]pyrene (BaP) in Gclm mice compared to Gclm+/+ littermates. In addition, the tumor histology has a more tubular appearance in the Gclm-/- mice. In this aim we will extend these preliminary observations and will utilize immunostaining for ovarian tumor markers to clarify the tumor type(s) and the ovarian cell type(s) from which the tumors may originate. Specific Aim 2, Effect of Gclm deletion on transplacental induction of ovarian mutations by BaP. Mutations in oncogenes and tumor suppressor genes are thought to play a role in the development of ovarian cancers in humans. In particular, mutations of the KRAS oncogene have been associated with mucinous epithelial ovarian tumors, the type most strongly associated with cigarette smoking. We hypothesize that reactive metabolites of BaP will cause nuclear DNA mutations, which are important in the early pathogenesis of ovarian tumors. Ovaries will be collected before tumors have developed from Gclm-/- and Gclm+/
The politics of women's legislative caucuses
Creators: name: Mahoney, Anna Mitchell
Publisher: name: No Publisher Supplied
Year: 2013

description: According to the Center for American Women and Politics, 1,&49 women are serving in state legislatures in 2012, making up 23.&% of legislators across the country. The impact of women in elected office has received much scholarly attention with analysis of the different priorities and legislative styles of women, but little research exists on the effects of women’s caucuses in the 50 states. My dissertation identifies opportunity structures and legislator motivations that facilitate the creation of women’s legislative caucuses. The project employs case studies to test hypotheses about caucus creation, original quantitative and qualitative research on the existence of women’s caucuses throughout the states, and a quantitative analysis of key variables and their relationships with women’s caucuses. I argue that women’s gendered identity influences the goals and structures of their organizing efforts within gendered political institutions. Beyond the traditional purpose of a caucus, to directly influence the policy process, these organizations offer women a supportive environment from which to challenge the status quo, which has sometimes served to exclude women from full participation in the legislature.
Additional file 2: of Molecular and serological detection of bovine babesiosis in Indonesia
Creators: name: Azirwan Guswanto
name: Puttik Allamanda
name: Euis Mariamah
name: Sodirun Sodirun
name: Putut Wibowo
name: Liliek Indrayani
name: Nugroho, Rudi
name: I Wirata
name: Jannah, Nur
name: Lepsi Dias
name: Wirawan, Hadi
name: Rochmadi Yanto
name: Bumduuren Tuvshintulga
name: Sivakumar, Thillaiampalam
name: Naoaki Yokoyama
name: Igarashi, Ikuo
Publisher: name: Figshare
Year: 2017
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Genetics
subject: Molecular Biology
subject: Biotechnology
subject: Evolutionary Biology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: Developmental Biology
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: 60506 Virology

description: Alignment of partial amino acid sequences of Babesia bovis SBP-4 (A), partial nucleotide sequences of B. bovis SBP-4 (B), partial amino acid sequences of B. bigemina RAP-1a (C), partial nucleotide sequences of B. bigemina RAP-1a (D), nucleotide sequences B. bovis ITS region (E), and nucleotide sequences B. bigemina ITS region (F) from Indonesian isolates. (DOCX 27Â kb)
Characterization of a novel tumor-suppressor gene in a simple animal model
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Endosomes have long been considered major stations for the intracellular sorting and distribution of many integral membrane proteins, including but not limited to growth hormone receptors. In addition, recent work has uncovered a role in the spatial organization of intracellular signaling circuits – the so-called “signaling endosome.” Key regulators of early endosome biogenesis and function include the small GTPase Rab5, which is activated by the guanine-nucleotide-exchange activity of proteins carrying the VPS9 domain. During the course of our studies on the role of the endocytic pathway in the biogenesis of cell-type-specific organelles, we serendipitously found that loss-of-function mutations in one of the four VPS9-domain-containing proteins encoded by the genome of the fruit fly, Drosophila melanogaster, lead to the development of tumors in brain and other tissues. The protein in question is encoded by the single fly ortholog of a human gene that had been loosely linked to cancer albeit not studied specifically as a tumor-suppressor gene. This seed grant request is for me to embark, for the first time in my career, upon a line of research clearly related to cancer. Specifically, I propose to take advantage of the potential of Drosophila as a simple, genetically tractable model organism to understand (1) why the lack of this particular VPS9-domain-containing protein may result in tumor development, and (2) whether this tumor-suppressor activity requires interaction with a binding partner that, in humans, has been likewise linked to cancer albeit not yet formally described as a tumor suppressor. The results from this project should lay the foundations for a more ambitious – likely collaborative – line of research, which as I envision should in the future expand to mammalian animal models and eventually humans.
Point Pleasant Beach, New Jersey : a comprehensive development plan.
Creators: name: No Name Supplied
Publisher: name: No Publisher Supplied
Year: 1963

description: This development plan is intended as a working guide to a Point Pleasant Beach that is a healthy, safe, functional and beautiful community both in the near and distant future. It contains summaries of the region, housing, borough, transportation, recreation, education, and services and utilities of Point Pleasant Beach, along with planning for capital improvements and urban renewal.
Lagrioida nortoni Costa, Vanin & Ide 1995
Creators: name: Groll, Elisa Von
Publisher: name: Zenodo
Year: 2017
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Coleoptera
Anthicidae
Lagrioida
Lagrioida nortoni

description: Lagrioida nortoni Costa, Vanin & Ide, 1995
Costa, Vanin & Ide, 1995:14, figs 1–43.
Paratypes ♂, ♀, BRAZIL, Rio Grande do Sul: Rio Grande (Praia do Cassino), 30.XI.1992, N. M. Gianuca col., associados às espiguilhas de Panicum racemosum (MCNZ 234.808, 234.807).
description: Published as part of Groll, Elisa Von, 2017, Catalogue of Coleoptera type specimens housed in the collection of the Museu de Ciências Naturais, Fundação Zoobotânica do Rio Grande do Sul, Brazil, pp. 439-473 in Zootaxa 4318 (3) on page 466, DOI: 10.11646/zootaxa.4318.3.2, http://zenodo.org/record/887750
title: When I Think About Myself
Creators: name: Witherspoon, Jazmin
Publisher: name: University of Kentucky Libraries
Year: 2024
Subjects: classificationCode: 6.2
subject: FOS: Languages and literature

description: When I Think About Myself is a collection of poetry written by Jazmin Witherspoon. They were compiled during her time at The University of Kentucky as a graduate student from 2022-2024. Her poetry explores themes of black identity politics, race, sexuality, and black girlhood.
description: 2024 Jazmin Witherspoon
Additional file 4: of Identification of a novel genetic locus underlying tremor and dystonia
Creators: name: Monies, Dorota
name: Al-Shaar, Hussam
name: Goljan, Ewa
name: Banan Al-Younes
name: Al-Breacan, Muna
name: Al-Saif, Maher
name: Wakil, Salma
name: Meyer, Brian
name: Khabar, Khalid
name: Bohlega, Saeed
Publisher: name: Figshare
Year: 2017
Subjects: subject: Space Science
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Evolutionary Biology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Marine Biology
subject: Infectious Diseases
subject: FOS: Health sciences

description: CAMTA2 expression in brain. (PDF 1628Â kb)
Pre-clinical studies of bladder cancer-targeting nanotechnology for improved imaging and therapeutics
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Bladder cancer is the fourth most common cancer in men and the ninth most common in women in the United States. The incidence of bladder cancer continues to rise and little improvement has been made in bladder cancer mortality in the last 20 years. Using one-bead-one-compound combinatorial chemistry, Dr. Chong Pan has identified the novel peptide PLZ4 with the amino acid sequence cQDGRMGFc that selectively binds human and dog bladder cancer cells, but does not bind to normal urothelial cells, blood cells, fibroblasts and vascular endothelial cells. Our hypothesis is that the novel peptide ligand PLZ4 can be used for enhanced selective imaging and treatment of non-muscle invasive bladder cancer. Specific Aim 1. To determine the in vivo ability of PLZ4-flourescent probe conjugates to target superficial bladder cancer for enhanced imaging of bladder tumors. Methods: We have established an orthotopic bladder cancer model for this study. For these experiments, red fluorescence protein (RFP) stably transfected bladder cancer cell lines 5637 will be used. PLZ4 peptide will be labeled with near-infrared fluorescent FITC for imaging purposes. Mice bearing orthotopic superficial bladder tumors will be anesthetized and catheterized for bladder instillation with fluorescent conjuagates. After the mice are sacrificed, the bladder is examined microscopically for development of cancer, and for targeting capacity of the ligand. The 5637 cancer cells appear red (transfected with RFP) and the binding of cancer-specific ligands green (labeled with FITC) under the fluorescence microscope. Specific Aim 2. To determine in vivo therapeutic efficacy of PLZ4-labeled nano-micelles loaded with chemotherapy for targeting superficial bladder cancer Methods: Mice bearing orthotopic superficial bladder tumors will be anesthetized and catheterized for bladder instillation with PLZ4 labeled nano-micelles loaded with the chemotherapeutic Paclitaxol. Mice will be sacrificed at 3 different time points, day 0, day 5, and day 10 after treatment. Bladders will be grossly inspected for presence of tumor and targeted anti-tumor activity will be assessed by measuring tumor size and weight, and compared to control groups. Drug delivery can be assessed and quantified by co-packaging micelles with fluorescent molecules.
Co-targeting genomic instability and energy metabolism in breast cancer
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Genomic instability is an “enabling” hallmark of cancer that permits the acquisition of cancer phenotypes that confer a selective advantage to subclones of tumor cells. Cancer cells often undergo a metabolic reprogramming that triggers prodigious consumption of glucose and glutamine and production of lactate. Our preliminary results suggest that these phenotypes are linked. Examining the energy metabolism genes in glycolytic primary breast cancers, we demonstrate an enrichment of DNA copy number alterations in genes with linked metabolic function (eg, glycolysis). This pattern suggests that coordinated amplification and deletion of energy metabolism genes in genomically unstable tumors provides a selective advantage during tumor evolution (ie, tumor-beneficial metabolism). Using models of basal-like breast cancer, we will determine if metabolite growth conditions can select for DNA copy number alterations in sets of genes with linked metabolic function. Additionally, the coupled nature of genomic instability and aberrant tumor metabolism raises the possibility that treatment of cancers with these phenotypes (eg, basal-like breast cancer) with cocktails of drugs targeting DNA repair and metabolism will prove more effective than either inhibitor alone. We will test these hypotheses to better understand selective forces during tumorigenesis and the potential for co-targeting genomic instability and oncogenic metabolism.
Regulation of the class III phosphatidylinositol 3-kinase in autophagic cell death
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Autophagy is a cellular degradation pathway that has a newly recognized role to prevent cancer formation, probably through eliminating damaged proteins and organelles. Genes involved in the control of autophagy have been newly identified as tumor suppressor genes. In autophagy, the class III PI3 kinase (PI3KC3), a lipid kinase that catalyzes the conversion of PtdIns to PtdIns3P, is the rate-limiting factor required for the autophagosomes formation and maturation. The gene encoding Beclin 1, a major regulatory component of this complex, is frequently monoallellically deleted in ovarian, breast and prostate cancers. Also, heterozygous Beclin 1 mice display an increased incidence of spontaneous tumors. Autophagy modulation thus provides a novel direction in drug design for the treatment of cancers. However, since Beclin 1 and PI3KC3 play a pleiotropic role in autophagic and endocytic pathways, it is critical to determine the functional mode and specificity of PI3KC3 in different pathways. We have recently discovered that PI3KC3 forms two mutually exclusive subcomplexes on autophagosomes and endosomes respectively, which might decide the specificity of PI3KC3. We propose that PI3KC3 regulates autophagy and cell growth by formation of organelle-specific subcomplexes. A mechanistic understanding of PI3KC3 regulation will help us decipher how autophagy becomes dysregulated in cancers and to identify potential new targets for therapeutic intervention.
Decision on the case Raw Story Media & AlterNet Media vs OpenAI (7 nov. 2024)
Creators: name: United States District Court - Southern District of New York
Publisher: name: Zenodo
Year: 2024

description: Decision and order by Judge McMahon of the United States District Court - Southern District of New York for the case between Raw Story Media & AlterNet Media (Palintiffs) and OpenAI (Defendant).

Italian translation available here.
Master plan, borough of Riverdale, New Jersey; Title vii master plan studies, report number 2
Creators: name: No Name Supplied
Publisher: name: No Publisher Supplied
Year: 1960

description: In the summer of 1959, we presented to the Planning Board a Report on existing conditions and analyzed problems of the Borough of Riverdale. This present report, the 1960 Master Plan, sets forth the objectives and, together with a zoning ordinance, subdivision ordinance, official map and capital improvements program, outlines a means of reaching those objectives.
Additional file 4: of Identification of a novel genetic locus underlying tremor and dystonia
Creators: name: Monies, Dorota
name: Al-Shaar, Hussam Abou
name: Goljan, Ewa
name: Banan Al-Younes
name: Al-Breacan, Muna
name: Al-Saif, Maher
name: Wakil, Salma
name: Meyer, Brian
name: Khabar, Khalid
name: Bohlega, Saeed
Publisher: name: Figshare
Year: 2017
Subjects: subject: Space Science
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Evolutionary Biology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Marine Biology
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: CAMTA2 expression in brain. (PDF 1628Â kb)
Rutherford master plan, report no. 2; Existing conditions: physical characteristics, existing land use, community facilities, lot frontage, economic base, fiscal studies
Creators: name: No Name Supplied
Publisher: name: No Publisher Supplied
Year: 1965

description: This 1965 master plan for the city of Rutherford, NJ covers the city's physical characteristics, existing land use, community facilities, lot frontage, economic base, and fiscal studies.
MillA dereplicated genomes
Creators: name: Nesbo, Camilla
name: Edwards, Elizabeth
name: Sharan, Anupama
Publisher: name: figshare
Year: 2024
Subjects: classificationCode: 310204
subject: Genomics and transcriptomics

description: Metagenome assembled genomes from the anaerobic digester at Mill A. The folder contains fasta files of the MAGs and an excel file with genome statistics from CheckM and Anvi'o.
Dorosoma chavesi Meek 1907
Creators: name: Angulo, Arturo
name: Betts, Joel T.
name: González-Alemán, Néstor J.
name: Castañeda, Edgar
name: Berghe, Eric Van Den
name: Elías, Diego J.
name: Mcmahan, Caleb D.
name: Matamoros, Wilfredo A.
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Chordata
Clupeiformes
Clupeidae
Dorosoma
Dorosoma chavesi

description: Dorosoma chavesi Meek 1907 (Fig. 6K). Nicaragua Gizzard Shad; Sardina, Sardinilla, Sabalete, Sardina de Río
Vouchers: UCR 0050-001 (5), 0061-003 (1), 0536-001 (6), 0542-005 (10), 0546-011 (9), 0547-003 (12), 0550-004 (15), 0552-001 (11), 0596-002 (10), 1065-005 (1) and 3115-011 (2); FMNH 5925 (15), 5926 (30), 5927 (6), 5928 (1), 14629 (1), 14630 (1), 14631 (1), 14632 (1), 14633 (1), 14634 (1), 14635 (1), 14636 (1), 14637 (1), 14638 (1), 14639 (1), 14640 (1), 52272 (7) and 126133 (6); other collections: CAS 61542 (1), TCWC 249202 (1), UF 108725 (1), 108741 (1), 108742 (1), 108743 (1), 108760 (1), 109843 (1), 135628 (1), 202738 (4), UMMZ 162482 (1), 179115 (1), YPM-ICH 008559 (9), 008560 (9), 008561 (7), 008562 (6) and 010236 (25). Distribution: Central America; from western Nicaragua (lakes Xolotlán and Cocibolca and affluent streams or rivers) to northern Costa Rica, Atlantic drainages; (Nicaragua) SJ, RF and LN (Atlantic); 0–50 masl; Per, Dia, Amp. Occurrence and conservation status: Nat; NT B1ab(iii) (2018), population trend unknown. Literature: Meek (1907: 112; brief description, including an identification key, and information on distribution and ecology), Astorqui (1975: 97; brief description, including illustrations and an identification key, and information on distribution and ecology), Villa (1982: 87; detailed description, including illustrations and an identification key, and information on distribution and ecology), Bussing (1998: 68; brief description, including illustrations and an identification key, and information on distribution, with a map, and ecology) and Angulo (2021: 22; listed, including information on distribution).
description: Published as part of Angulo, Arturo, Betts, Joel T., González-Alemán, Néstor J., Castañeda, Edgar, Berghe, Eric Van Den, Elías, Diego J., Mcmahan, Caleb D. & Matamoros, Wilfredo A., 2023, Continental fishes of Nicaragua: diversity, distribution and conservation status; with an annotated and illustrated checklist of species and an identification guide to families, pp. 1-89 in Zootaxa 5376 (1) on page 19, DOI: 10.11646/zootaxa.5376.1.1, http://zenodo.org/record/10208788
Examining the role of Fe-S cluster biogenesis in the assembly of DNA repair complexes
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Dysregulation of the pathways that govern DNA repair and the maintenance of genomic stability is a common feature of many cancers. Despite their central role in tumorigenesis, many aspects of the regulation of these DNA repair pathways remains to be elucidated. Work in recent years has identified an essential role for iron-sulfur clusters in the proper functioning of many DNA repair enzymes including the XPD family of DNA helicases. These small inorganic cofactors are incorporated into target cytoplasmic and nuclear proteins through the activity of the recently identified cytoplasmic iron assembly (CIA) pathway. My laboratory has discovered a novel protein complex that we have termed the MCFB complex that functions at a late stage in the CIA pathway to facilitate the assembly of iron-sulfur clusters into many of these iron-dependent DNA repair enzymes. Using the DNA helicase XPD as a model Fe-S enzyme, we have shown that MCFB is required for XPD activity and its assembly into functional TFIIH complexes. In the proposed work, we will expand our biochemical analysis of this novel pathway. Specifically, we will examine the effects of MCFB depletion on the iron binding, helicase, and DNA repair activities of XPD and TFIIH. We will also utilize advanced proteomic methods to assess whether the role of MCFB in the proper assembly of DNA repair complexes can be generalized beyond XPD and TFIIH. Together, the proposed work will examine a potential role for the MCFB complex as a master regulator of DNA repair pathways whose dysfunction can severely disrupt the ability of cells to respond to genotoxic stress. Ultimately, we hope that that this work will yield insight into the potential therapeutic benefits of using small molecules to chemically target MCFB and the iron-sulfur cluster assembly machinery.
Bledius hermani Caron & Ribeiro-Costa 2007
Creators: name: Groll, Elisa Von
Publisher: name: Zenodo
Year: 2017
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Coleoptera
Staphylinidae
Bledius
Bledius hermani

description: Bledius hermani Caron & Ribeiro-Costa, 2007
Caron & Ribeiro-Costa, 2007:455, figs 17–24.
Paratypes, 10 specimens, BRAZIL, Paraná: Guaraqueçaba (Superagui), 20.IX.2006, Borzone col. (MCNZ 235.488, 235.489, 235.490, 235.491, 235.492, 235.493, 235.494, 235.495, 235.496, 235.497).
description: Published as part of Groll, Elisa Von, 2017, Catalogue of Coleoptera type specimens housed in the collection of the Museu de Ciências Naturais, Fundação Zoobotânica do Rio Grande do Sul, Brazil, pp. 439-473 in Zootaxa 4318 (3) on page 466, DOI: 10.11646/zootaxa.4318.3.2, http://zenodo.org/record/887750
Development of a decision support system to operate the greenhouse lighting and shading systems powered by a distributed generator
Creators: name: Martin, Ariel
Publisher: name: No Publisher Supplied
Year: 2013

description: A Decision Support System (DSS) was developed to manage the use of the electric- ity generated by a landfill gas-fired 250 kW microturbine system installed at the Rut- gers EcoComplex Research and Demonstration Greenhouse located at the Burlington County Resource Recovery Complex near Columbus, NJ. The approximately 220 kW of available power (30 kW are parasitic losses) can be used on-site for supplemental lighting of the greenhouse crop, or it can be exported to the local electricity grid.  In order to maintain sufficiently high crop production during the winter months and darker stretches during other times of the year, supplemental lighting is necessary particularly in the Northern regions of the country. Due to the relatively high light intensity required, supplemental lighting of greenhouse crops can be expensive. The DSS minimizes the cost of the electricity associated with supplemental lighting and guarantees that the harvest date and yield targets are achieved.  The DSS can be implemented at any location within the geographic area of PJM (regional transmission organization that coordinates the wholesale electricity market in 13 states, including NJ) and it was validated by simulation using five years of his- toric hourly values of electricity prices from PJM and hourly values of solar radiation from the National Solar Radiation Database (NSRDB).  The simulations results show that the DSS is able to effectively manage different possible configurations of the supplemental lighting and shading screen systems to meet a predetermined harvest date and yield while minimizing the use of the on-site generated electricity. All of the excess electricity is exported to the grid in such a way as to maximize the additional income from electricity sales.
Harengula jaguana Poey 1865
Creators: name: Angulo, Arturo
name: Betts, Joel T.
name: González-Alemán, Néstor J.
name: Castañeda, Edgar
name: Berghe, Eric Van Den
name: Elías, Diego J.
name: Mcmahan, Caleb D.
name: Matamoros, Wilfredo A.
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Chordata
Clupeiformes
Clupeidae
Harengula
Harengula jaguana

description: Harengula jaguana Poey 1865. Scaled Herring, Scaled Sardine; Sardina Escamudina, Sardineta Jaguana, Sardinita Vivita Escamuda
Vouchers: Not available. Distribution: Western Atlantic Ocean; from New Jersey, USA, to southern Brazil; entering estuaries and littoral lagoons and ascending river courses; (Nicaragua) WK, KP, Es, BF, IM and To (Atlantic); 0–5 masl; Per, Dia, Amp. Occurrence and conservation status: Nat; LC (2019), population trend unknown.
Literature: Villa (1982: 88, as Harengula pensacolae Goode & Bean 1879 —synonym; detailed description, including illustrations and an identification key, and information on distribution and ecology) and Robertson & Van Tassell (2019: 2921; detailed description, including illustrations, and information on distribution, with a map, and ecology).
description: Published as part of Angulo, Arturo, Betts, Joel T., González-Alemán, Néstor J., Castañeda, Edgar, Berghe, Eric Van Den, Elías, Diego J., Mcmahan, Caleb D. & Matamoros, Wilfredo A., 2023, Continental fishes of Nicaragua: diversity, distribution and conservation status; with an annotated and illustrated checklist of species and an identification guide to families, pp. 1-89 in Zootaxa 5376 (1) on page 19, DOI: 10.11646/zootaxa.5376.1.1, http://zenodo.org/record/10208788
Experimental validation of a predictive model for Salmonellagrowth In raw ground beef under dynamic temperatures
Creators: name: McConnell, Jennifer A.
Publisher: name: No Publisher Supplied
Year: 2013

description: When food is transported at ambient temperatures for extended periods of time, or when power is lost during natural disasters; foodborne pathogens can multiply.  Current US Food and Drug Administration (FDA) Model Food Code guidelines state that food can be kept out of temperature control for up to 4 h or up to 6 h if the food product starts at an initial 41°F (5°C) and the temperature does not exceed &0°F (21°C).  This project validates existing ComBase computer models for Salmonella spp. growth under changing temperature conditions in raw ground beef as model system, using scenarios that would exceed Food Code guidelines.  This thesis is separated into a literature review describing FDA Model Food Code guidelines, Salmonella prevalence and concentration in ground beef, and dynamic models for bacterial growth (I) and experimental validation of ComBase computer models for Salmonella spp. growth in raw ground beef (II).  The growth rate of a 5-strain cocktail of Salmonella spp. meat isolates was inoculated in 20% fat ground beef at a concentration of 4-log CFU/g.   Inoculated ground beef samples were temperature abused for different lengths of time and to different maximum temperatures.  The temperature profiles represent loss of proper refrigeration, warming and then cooling following a linear temperature gradient.  A total of 9 different conditions were studied.  Results show that when maximum temperatures were low, there was generally good agreement between the ComBase models and experiments.  When maximum temperatures were closer to the optimum growth temperature for Salmonella (3&°C), predictive models were fail-safe.  It appears that faster cooling times limit the growth of Salmonella, so rapidly cooling foods (e.g. in a freezer) after extended temperature abuse can work as a risk mitigation measure.  Validation of these models will be useful to extension professionals advising consumers, restaurateurs transporting food in unrefrigerated vehicles, and retailers facing a power outage.  These finding may also be useful to those seeking to improve the science base of the FDA Model Food Code.
Magmatic evolution of the north Tanzanian divergence zone, East African rift system
Creators: name: Mana, Sara
Publisher: name: No Publisher Supplied
Year: 2013

description: My study focuses on the magmatic evolution of the North Tanzanian Divergence zone (NTD). Using high precision 40Ar/39Ar geochronology I identify stages of magmatic activity and episodes of progressive magma migration. The newly developed temporal framework combined with elemental and isotopic geochemistry allows me to understand how the melting process has changed during the spatial and temporal evolution of the NTD. I use the abundances of key trace elements to identify the source mineralogy; including rare earth element (REE) modeling to constrain depths of melt separation. In addition, I employ radiogenic Sr-Nd-Pb isotope ratios to identify possible contributions from different mantle and crustal components.   The NTD represents an example of an early stage continental rifting where lithospheric melts are generated during the interaction of a mantle plume and a heterogeneous metasomatized lithosphere. Melting occurs in the garnet stability zone. The first melt produced derives from fusible amphibole rich veins. Through the NTD rifting development, melting conditions change to generate alkali basalts with systematically different isotopic signatures. This episode of magmatism consists of an intense pulse, related to the inception of rifting, and characterized by melt separation occurring at shallower depths. The observed chemical signature can be explained either with the involvement of contributions from plume related fluids, or by melting of layered lithosphere. Subsequent volcanism has features consistent with mixing between the first two events. Through time we observe an eastwards migration of the magmatic activity consistent with the hypothesis of a fixed mantle plume and an overall westward migration of the African plate.
CTP synthase polymerization: a novel mechanism for regulating nucleotide metabolism
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: The microtubule cytoskeleton and nucleotide biosynthetic pathways are the two major targets of existing cancer chemotherapy agents. We have recently conducted a visual screen of the yeast GFP strain collection to identify novel filament forming proteins. This screen identified nine filament-forming proteins, most of which are proteins involved in intermediate metabolism. Surprisingly, one of these proteins is CTP synthase, which forms filaments in yeast, Drosophila, and mammalian cells. Our initial in vivo studies of CTP synthase polymerization have found that filament formation is triggered by end-product inhibition of the enzyme by CTP. Since CTP synthase is the only enzyme capable of de novo CTP synthesis, it has long been a target of interest for developing new chemotherapeutic agents. Our findings suggest a novel strategy for developing CTP synthase inhibitors that combines aspect of the two major classes of chemotherapy agents– the identification of compounds that inhibit CTP synthase activity by promoting polymerization. The goal of this proposal is to combine biochemical and genetic analysis to define the mechanism of CTP synthase polymerization and its relationship to enzyme activity. Aim 1 of this proposal is to use genetic analysis combined with live imaging to identify the key amino acids required for CTP synthase polymerization. Mapping these mutations to the known structure of CTP synthase will allow us to define the surfaces of the protein implicated in polymerization providing a roadmap for designing small molecules that can alter polymerization. Aim 2 of this proposal is focused on using existing assays for CTP synthase activity to determine the effects of polymerization mutants on enzyme activity. As part of these studies we will also develop an in vitro polymerization assays for yeast CTP synthase. These assays will allow us to characterize polymerization defective mutants identified in Aim 1 and provide a basis for developing screens for small molecules that alter polymerization. Funding of this proposal will provide novel insights into a potential chemotherapy target implicated in both cellular growth and chemotherapy resistance - CTP synthase.