title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ Ð°Ð½ÑÐ¸Ð±Ð¸Ð¾ÑÐ¸ÐºÐ° ÑÐµÑÐ¼Ð¾ÑÑÐ±Ð¸Ð½Ð° Ð½Ð° ÑÑÐ°Ð¿Ñ ÑÐ¸ÐºÐ»Ð° ÑÐ»Ð¾Ð½Ð³Ð°ÑÐ¸Ð¸ ÑÐ¸Ð½ÑÐµÐ·Ð° Ð±ÐµÐ»ÐºÐ° Ð¿ÑÐ¾ÐºÐ°ÑÐ¸Ð¾Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: The effect of the antibiotic thermorubin on the stages of the prokaryotic protein synthesis elongation cycle
Creators: name: ÐÑÐ°ÑÐµÐ², ÐÐ»ÐµÐºÑÐ°Ð½Ð´Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÑÐµÑÐ¼Ð¾ÑÑÐ±Ð¸Ð½
Ð±Ð¸Ð¾ÑÐ¸Ð½ÑÐµÐ· Ð±ÐµÐ»ÐºÐ°
ÑÐ¸Ð±Ð¾ÑÐ¾Ð¼Ð½ÑÐµ Ð°Ð½ÑÐ¸Ð±Ð¸Ð¾ÑÐ¸ÐºÐ¸
ÑÐ¸ÐºÐ» ÑÐ»Ð¾Ð½Ð³Ð°ÑÐ¸Ð¸
Ð¿ÑÐµÑÑÐ°ÑÐ¸Ð¾Ð½Ð°ÑÐ½Ð°Ñ ÐºÐ¸Ð½ÐµÑÐ¸ÐºÐ°
Ð¿ÐµÐ¿ÑÐ¸Ð´Ð½Ð°Ñ ÑÐ²ÑÐ·Ñ
thermorubin
protein biosynthesis
ribosomal antibiotics
elongation cycle
rapid kinetics
peptide bond

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description: The subject of the graduate qualification work is âThe effect of the antibiotic thermorubin on the stages of the prokaryotic protein synthesis elongation cycle. âThe given work is devoted to the study of the effect of the ribosomal antibiotic thermorubin on the 3 main stages of the prokaryotic translation elongation cycle in vitro: aminoacyl-tRNA A-site binding, peptide bond formation and translocation. The apparent rate constants of tRNA binding and translocation were determined by the stopped flow method. In addition, the effect of the antibiotic on the ability of ribosomes to provide a peptidyl transferase reaction was determined and the thermodynamic contribution made by thermorubin to the metastable interaction of peptidyl-tRNA with the A site of the ribosome was evaluated. The main methods used in this work are in vitro reconstruction of the E. coli protein synthesis system, measurement of rapid kinetics parameters by the stopped flow method, high-performance liquid chromatography in reversed phases, radioluminescent spectral analysis.