title: Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° ÑÐ¸ÑÑÐµÐ¼Ñ ÑÐ¿ÑÐ°Ð²Ð»ÐµÐ½Ð¸Ñ Ð±Ð»Ð¾ÐºÐ¾Ð¼ Ð¿ÑÐ¾Ð±Ð¾Ð¿Ð¾Ð´Ð³Ð¾ÑÐ¾Ð²ÐºÐ¸ Ð¾Ð´Ð½Ð¾Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾Ð³Ð¾ ÑÐµÐºÐ²ÐµÐ½Ð°ÑÐ¾ÑÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Development of Sample preparation unit for SMRT sequencing
Creators: name: Ð¯Ð¼Ð°Ð½Ð¾Ð²ÑÐºÐ°Ñ, ÐÐ½Ð°ÑÑÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Ð¾Ð´Ð½Ð¾Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾Ðµ ÑÐµÐºÐ²ÐµÐ½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
ÑÐµÐºÐ²ÐµÐ½Ð°ÑÐ¾Ñ
Ð¿ÑÐ¾Ð±Ð¾Ð¿Ð¾Ð´Ð³Ð¾ÑÐ¾Ð²ÐºÐ°
Ð°Ð²ÑÐ¾Ð¼Ð°ÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð´Ð¾Ð·Ð°ÑÐ¾Ñ
Ð´Ð¾Ð·Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
ÑÐµÑÐ¼Ð¾ÑÑÐ°Ñ
Ð²Ð¾Ð»Ð½Ð¾Ð²Ð¾Ð´Ñ Ð½ÑÐ»ÐµÐ²Ð¾Ð¹ Ð¼Ð¾Ð´Ñ
single-molecular real-time sequencing
SMRT
sequencer
sample preparation
automatic dispenser
dosing
thermostat
ZMW
magnetic beads

description: ÐÐ±ÑÐµÐºÑÐ¾Ð¼ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¼Ð°Ð³Ð¸ÑÑÐµÑÑÐºÐ¾Ð¹ Ð´Ð¸ÑÑÐµÑÑÐ°ÑÐ¸Ð¸ ÑÐ²Ð»ÑÐµÑÑÑ ÑÐ¸ÑÑÐµÐ¼Ð° ÑÐ¿ÑÐ°Ð²Ð»ÐµÐ½Ð¸Ñ Ð¿Ð¾Ð´Ð³Ð¾ÑÐ¾Ð²ÐºÐ¾Ð¹ Ð¿ÑÐ¾Ð±Ñ Ðº ÑÐµÐºÐ²ÐµÐ½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ SMRT. ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° ÑÐ¾Ð·Ð´Ð°Ð½Ð¸Ñ Ð¼Ð°ÐºÐµÑÐ° Ð¸ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ð½Ð¾Ð³Ð¾ Ð¾Ð±ÐµÑÐ¿ÐµÑÐµÐ½Ð¸Ñ Ð±Ð»Ð¾ÐºÐ° Ð¿ÑÐ¾Ð±Ð¾Ð¿Ð¾Ð´Ð³Ð¾ÑÐ¾Ð²ÐºÐ¸ Ð¾Ð´Ð½Ð¾Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾Ð³Ð¾ ÑÐµÐºÐ²ÐµÐ½Ð°ÑÐ¾ÑÐ° â Ð¿ÑÐ¸Ð±Ð¾ÑÐ° Ð´Ð»Ñ ÑÑÐµÐ½Ð¸Ñ ÐÐÐ Ð½Ð¾Ð²ÐµÐ¹ÑÐµÐ³Ð¾ Ð¿Ð¾ÐºÐ¾Ð»ÐµÐ½Ð¸Ñ. Ð¦ÐµÐ»ÑÑ Ð¼Ð°Ð³Ð¸ÑÑÐµÑÑÐºÐ¾Ð¹ Ð´Ð¸ÑÑÐµÑÑÐ°ÑÐ¸Ð¸ ÑÐ²Ð»ÑÐµÑÑÑ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° ÑÐ¸ÑÑÐµÐ¼Ñ ÑÐ¿ÑÐ°Ð²Ð»ÐµÐ½Ð¸Ñ Ð±Ð»Ð¾ÐºÐ¾Ð¼ Ð¿ÑÐ¾Ð±Ð¾Ð¿Ð¾Ð´Ð³Ð¾ÑÐ¾Ð²ÐºÐ¸ Ð´Ð»Ñ Ð¼Ð°ÐºÐµÑÐ° Ð¾Ð´Ð½Ð¾Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾Ð³Ð¾ ÑÐµÐºÐ²ÐµÐ½Ð°ÑÐ¾ÑÐ°. Ð Ð¿ÐµÑÐ²Ð¾Ð¹ Ð³Ð»Ð°Ð²Ðµ Ð¾Ð¿Ð¸ÑÐ°Ð½Ñ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½ÑÑ Ð¸ÑÑÐ¾ÑÐ½Ð¸ÐºÐ¾Ð² Ð¾ Ð¼ÐµÑÐ¾Ð´Ðµ Ð¾Ð´Ð½Ð¾Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾Ð³Ð¾ ÑÐµÐºÐ²ÐµÐ½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ, Ð¿ÑÐ¸Ð±Ð¾ÑÐ°Ñ-Ð¿ÑÐ¾ÑÐ¾ÑÐ¸Ð¿Ð°Ñ, Ð¼ÐµÑÐ¾Ð´Ð°Ñ Ð·Ð°Ð³ÑÑÐ·ÐºÐ¸ Ð¾Ð±ÑÐ°Ð·ÑÐ° Ð² Ð½Ð°Ð½Ð¾ÐºÐ¾Ð»Ð¾Ð´ÑÑ, ÐºÐ¾ÑÐ¾ÑÑÐµ Ð²Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÐ²Ð¸Ð¸ Ð»ÐµÐ³Ð»Ð¸ Ð² Ð¾ÑÐ½Ð¾Ð²Ñ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ¸ Ð¿Ð¾Ð²ÐµÐ´ÐµÐ½ÑÐµÑÐºÐ¾Ð¹ Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð¿ÑÐ¸Ð±Ð¾ÑÐ° Ð½Ð° ÑÑÐ°Ð´Ð¸Ð¸ ÑÑÐºÐ¸Ð·Ð½Ð¾Ð³Ð¾ Ð¿ÑÐ¾ÐµÐºÑÐ°. ÐÐ¾ Ð²ÑÐ¾ÑÐ¾Ð¹ Ð³Ð»Ð°Ð²Ðµ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÐµÐ½Ñ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ¸ ÑÑÐµÐ¼Ñ ÑÐ»ÐµÐºÑÑÐ¸ÑÐµÑÐºÐ¾Ð¹ ÑÑÑÑÐºÑÑÑÐ½Ð¾Ð¹, ÑÑÐµÐ¼Ñ ÑÐ»ÐµÐºÑÑÐ¸ÑÐµÑÐºÐ¾Ð¹ ÑÐ¾ÐµÐ´Ð¸Ð½ÐµÐ½Ð¸Ð¹, ÐÐ Ð¸ Ð¼ÐµÑÐ¾Ð´Ð¸ÐºÐ¸ Ð¸ÑÐ¿ÑÑÐ°Ð½Ð¸Ð¹ Ð±Ð»Ð¾ÐºÐ° Ð¿ÑÐ¾Ð±Ð¾Ð¿Ð¾Ð´Ð³Ð¾ÑÐ¾Ð²ÐºÐ¸ Ð¾Ð´Ð½Ð¾Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾Ð³Ð¾ ÑÐµÐºÐ²ÐµÐ½Ð°ÑÐ¾ÑÐ° Ð½Ð° ÑÑÐ°Ð´Ð¸Ð¸ ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¿ÑÐ¾ÐµÐºÑÐ°. Ð ÑÑÐµÑÑÐµÐ¹ Ð³Ð»Ð°Ð²Ðµ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÐµÐ½Ñ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð¸Ð·Ð¼ÐµÑÐµÐ½Ð¸Ð¹ Ð´Ð»Ñ Ð¿Ð¾Ð´ÑÐ²ÐµÑÐ¶Ð´ÐµÐ½Ð¸Ñ ÑÐ°Ð±Ð¾ÑÐ¾ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÐ¸ Ð¼Ð°ÐºÐµÑÐ° Ð² ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²Ð¸Ð¸ Ñ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð½Ð¾Ð¹ Ð¼ÐµÑÐ¾Ð´Ð¸ÐºÐ¾Ð¹. ÐÑÐµÐ´ÑÑÐ°Ð²Ð»ÐµÐ½Ð½ÑÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð°Ð¿ÑÐ¾Ð±Ð¸ÑÐ¾Ð²Ð°Ð½Ñ Ð² Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÑÐºÐ¾Ð¹ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ðµ ÐÐÐ Ð ÐÐ Ð¿Ð¾ ÑÐµÐ¼Ðµ Â«Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð¾Ð¿ÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±ÑÐ°Ð·ÑÐ° Ð¾Ð´Ð½Ð¾Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾Ð³Ð¾ ÑÐµÐºÐ²ÐµÐ½Ð°ÑÐ¾ÑÐ° ÐÐÐ, Ð½Ð°Ð±Ð¾ÑÐ° ÑÐµÐ°Ð³ÐµÐ½ÑÐ¾Ð² Ð¸ ÑÐ°ÑÑÐ¾Ð´Ð½ÑÑ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ð¾Ð² Ð´Ð»Ñ Ð½ÐµÐ³Ð¾Â» Ð¿ÑÐ¸ ÑÐ¸Ð½Ð°Ð½ÑÐ¾Ð²Ð¾Ð¹ Ð¿Ð¾Ð´Ð´ÐµÑÐ¶ÐºÐµ Ð¤ÐµÐ´ÐµÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð½Ð°ÑÑÐ½Ð¾-ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ñ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ñ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¹ Ð½Ð° 2019 â 2027 Ð³Ð¾Ð´Ñ Ð² ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²Ð¸Ð¸ Ñ ÑÐ¾Ð³Ð»Ð°ÑÐµÐ½Ð¸ÐµÐ¼ â 075-15-2021-1057 Ð¾Ñ 28.09.2021.
description: The given work is devoted to sample preparation unit of a single-molecule sequencer â the latest generation device for reading DNA. The first chapter describes the results of the single-molecule sequencing literature review, prototype devices and methods for loading a sample into ZMW. Performed materials allowed to introduce the behavioral model of the device. The second chapter presents a block diagram, wiring diagram, software and test methods for the sample preparation unit of a single-molecule sequencer at the stage of a technical project. The third chapter presents the results of experimental measurements to confirm the performance of the unit in accordance with the developed methodology. The study was approbated at the project "Development of a prototype of a single-molecule DNA sequencer, a set of reagents and consumables", which was carried out with a grant from the Federal scientific and technical program for the development of genetic technologies for 2019 - 2027, in accordance with agreement No. 075-15-2021-1057 dated September 28, 2021.