title: Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð¿ÑÐ¾ÑÐ¾ÐºÐ¾Ð»Ð° ÐºÐ°Ð»ÑÑÐ¸ÐµÐ²Ð¾Ð³Ð¾ Ð¸Ð¼Ð¸Ð´Ð¶Ð¸Ð½Ð³Ð° Ð´Ð»Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¾ÑÐ²ÐµÑÐ¾Ð² Ð½ÐµÐ¹ÑÐ¾Ð½Ð¾Ð² ÑÐµÑÑÐ°ÑÐºÐ¸ Ð½Ð° Ð¼Ð°Ð³Ð½Ð¸ÑÐ½Ð¾Ðµ Ð¿Ð¾Ð»Ðµ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Developing of calcium imaging protocol to study responses of retinal neurons to a magnetic field
Creators: name: ÐÐµÑÐµÐ»Ð¾Ð², Ð¯ÐºÐ¾Ð²
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð¼Ð°Ð³Ð½Ð¸ÑÐ½ÑÐ¹ ÐºÐ¾Ð¼Ð¿Ð°Ñ
ÐºÐ°Ð»ÑÑÐ¸ÐµÐ²ÑÐ¹ Ð¸Ð¼Ð¸Ð´Ð¶Ð¸Ð½Ð³
Ð¼Ð°Ð³Ð½Ð¸ÑÐ¾ÑÐµÑÐµÐ¿ÑÐ¸Ñ
ÑÐµÑÑÐ°ÑÐºÐ°
Ð³Ð°Ð½Ð³Ð»Ð¸Ð¾Ð·Ð½ÑÐµ ÐºÐ»ÐµÑÐºÐ¸
Ð²Ð½ÑÑÑÐµÐ½Ð½ÑÑ Ð¿Ð¾Ð³ÑÐ°Ð½Ð¸ÑÐ½Ð°Ñ Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ð°
ÑÐ»ÐµÐºÑÑÐ¾Ð¿Ð¾ÑÐ°ÑÐ¸Ñ
magnetic compass
calcium imaging
magnetoreception
retina
ganglion cells
inner limiting membrane
electroporation

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description: The project focuses on the selection, evaluation and improvement of protocols for loading calcium dyes into retinal neurons to study possible calcium responses to different modulations of the magnetic field around the preparation. By comparing a- and b-wave of electroretinographic responses of isolated retinas at different electroporation parameters used to deliver calcium dyes for calcium imaging, we found that low-voltage electroporation should be preferred. However, for preparations of each taxonomic group of animals, these voltages should be selected individually. The selected dye loading protocols were shown to can be used to record ex vivo calcium responses in frog and pigeon retinas. In this work, changing magnetic fields were used as the varying stimulus. Different patterns of calcium responses were recorded for the frog retinal preparation, comparable to the characteristics of calcium waves obtained in other studies. In the future, selected protocols with analysis of detectable calcium responses of retinal neurons and their frequency spectra can be used for magnetic compass studying in amphibians and birds.