Digitalis lanata subsp. leaves L.
Creators: name: Tropper, Marina
name: H, Stephanie
name: ohn
name: Wolf, Laura-Sophie
name: Fritsch, Julia
name: Kastner-Detter, Nina
name: Rieck, Christoph
name: Munkert, Jennifer
name: Meitinger, Nadine
name: Lanig, Harald
name: Kreis, Wolfgang
Publisher: name: Zenodo
Year: 2021
Subjects: Biodiversity
Taxonomy
Plantae
Tracheophyta
Magnoliopsida
Lamiales
Plantaginaceae
Digitalis
Digitalis lanata

description: 4.6. Protein purification from fresh young Digitalis lanata leaves
Crude extracts were prepared from fresh young leaves of D. lanata homogenized in an extraction buffer of 50 mM sodium phosphate, 30 mM β- mercaptoethanol, 20% polyvinylpolypyrrolidone (w/w), pH 7.5 at a ratio of 1:4 (w/V). The extract was stirred for 30 min and subsequently filtered through 4 layers of Miracloth (Merck KGaA, Darmstadt, Germany) before centrifugation at 48,000× g for 20 min.
Ammonium sulfate was added to the crude extracts and stirred for 30 min to obtain precipitates of 0–15%, 15–40%, 40–55% and 55–100% salt saturation. After centrifugation at 48,000× g for 20 min, the pellets were dissolved in phosphate buffer (pH 7.5, 50 mM, 30 mM β- mercaptoethanol).
The 40–55% and 55–100% precipitates were applied to hydrophobic interaction chromatography on Phenylsepharose FF 16/10 (low sub) (GE Healthcare Bio-Sciences AB, Sweden) equilibrated with 4 column volumes of buffer A (50 mM sodium phosphate, 30 mM β- mercaptoethanol, 1 M (NH 4) 2 SO 4, pH 7.5). Fractions of 10 ml were collected at a flow rate of 2.0 ml min 1. Unbound proteins were flushed with 7 column volumes of buffer A and bound proteins were eluted by a decreasing salinity gradient of 0–100% of buffer B (50 mM sodium phosphate, 30 mM β- mercaptoethanol) followed by 7 column volumes of 100% buffer B.
Fractions were tested for enzyme activity and the most active fractions were pooled and applied consecutively to ion exchange chromatography on Resource Q 1, ml (GE Healthcare Bio-Sciences AB, Sweden) at pH 8.0 and pH 6.5 and to Resource S, 1 ml (GE Healthcare Bio-Sciences AB, Sweden) at pH 5.0 for purification of the 55–100% precipitate. The precipitate containing 40–55% salt saturation was applied to Resource Q 1 ml (GE Healthcare Bio-Sciences AB, Sweden) at pH 8.0 and pH 8.5. The buffer of the protein solutions was exchanged before each chromatographic step using Amicon Ultra Centrifugal filter devices 1000 MWCO 20 ml (Millipore Carrightwohill, Ireland). The columns were equilibrated with 4 column volumes of appropriate buffer A (Table S1). The flow rate was 4.0 ml min 1 and eluates were collected in fractions of 0.5 ml. The column was washed with 10 column volumes of buffer A, and bound proteins were eluted by a gradient of 0–50% 1 M NaCl over 15 column volumes followed by 8 column volumes of 100% 1 M NaCl.Prior to SEC on Superdex 75 10/300_GL (GE Healthcare Bio-Sciences AB, Sweden), the protein solution was concentrated to a sample volume of 200 μl using Amicon Ultra Centrifugal filter devices 1000 MWCO 20 ml (Millipore Carrightwohill, Ireland). The column was equilibrated with 4 column volumes of sodium phosphate buffer (50 mM, 30 mM β- mercaptoethanol, 0.15 M NaCl, pH 7.5). The flow rate was set to 0.4 ml min 1 and fractions of 250 μl were collected. Proteins were eluted isocratically with 1.5 column volumes of buffer.
description: Published as part of Tropper, Marina, H, Stephanie, ohn, Wolf, Laura-Sophie, Fritsch, Julia, Kastner-Detter, Nina, Rieck, Christoph, Munkert, Jennifer, Meitinger, Nadine, Lanig, Harald & Kreis, Wolfgang, 2021, 21 - Hydroxypregnane 21 - O-malonylation, a crucial step in cardenolide biosynthesis, can be achieved by substrate-promiscuous BAHD-type phenolic glucoside malonyltransferases from Arabidopsis thaliana and homolog proteins from Digitalis lanata, pp. 1-10 in Phytochemistry (112710) (112710) 187 on page 8, DOI: 10.1016/j.phytochem.2021.112710, http://zenodo.org/record/8259095