title: ÐÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÑÑÐ°Ð¼Ð¼Ð°-Ð¿ÑÐ¾Ð´ÑÑÐµÐ½ÑÐ° Escherichia coli Ñ ÑÐµÐ»ÑÑ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ñ ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ñ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÑ ÑÐ½Ð´Ð¾ÑÐ¾ÐºÑÐ¸Ð½Ð¾Ð²
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Cultivation of the producer strain E. coli in order to reduce the content of bacterial endotoxins
Creators: name: Ð¯ÑÐ¼ÐµÐ½ÐµÐ²Ð°, ÐÐ½Ð³ÐµÐ»Ð¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: e. coli
Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÐµ ÑÐ½Ð´Ð¾ÑÐ¾ÐºÑÐ¸Ð½Ñ
lal-ÑÐµÑÑ
ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ñ
bacterial endotoxins
lal-test
culturing
concentration
biosynthesis

description: ÐÑÐ¸ Ð½Ð°Ð¿Ð¸ÑÐ°Ð½Ð¸Ðµ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±Ð·Ð¾ÑÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ: ÐÐ¸Ð±ÐµÑ-ÐÐµÐ½Ð¸Ð½ÐºÐ°, Ð­ÐÐ¡ ÐÐ¹Ð±ÑÐºÑ.ÑÑ, elib.spbstu.ru, eLIBRARY.RU, PudMed. Ð¡ÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÑÑ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»Ð¸ Ñ Ð¸Ñ-Ð¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿Ð°ÐºÐµÑÐ° Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼: Microsoft Excel. Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: ÐÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÑÑÐ°Ð¼Ð¼Ð°-Ð¿ÑÐ¾Ð´ÑÑÐµÐ½ÑÐ° E. coli Ñ ÑÐµÐ»ÑÑ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ñ ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ñ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÑ ÑÐ½Ð´Ð¾ÑÐ¾ÐºÑÐ¸Ð½Ð¾Ð². Ð¦ÐµÐ»Ñ ÑÐ°Ð±Ð¾ÑÑ: ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°ÑÑ Ð¿ÑÐ¾Ð±Ð»ÐµÐ¼Ñ Ð² ÑÐ°Ð±Ð¾ÑÐµ Ñ E. coli â Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ðµ ÑÐ½Ð´Ð¾-ÑÐ¾ÐºÑÐ¸Ð½Ð¾Ð² Ð¸ Ð¼ÐµÑÐ¾Ð´Ñ ÑÐ¼ÐµÐ½ÑÑÐµÐ½Ð¸Ñ Ð¸Ñ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸. ÐÐ°Ð´Ð°ÑÐ¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: - Ð¿ÑÐ¾Ð²ÐµÑÑÐ¸ Ð¾Ð±Ð·Ð¾Ñ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÑ; - Ð¿Ð¾Ð´Ð¾Ð±ÑÐ°ÑÑ Ð¼ÐµÑÐ¾Ð´Ð¸ÐºÐ¸ Ð´Ð»Ñ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð¸Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹; - Ð¸Ð·ÑÑÐ¸ÑÑ ÑÐ¿Ð¾ÑÐ¾Ð±Ñ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ ÑÐ½Ð´Ð¾ÑÐ¾ÐºÑÐ¸Ð½Ð¾Ð²; - Ð½Ð°Ð¹ÑÐ¸ Ð¼ÐµÑÐ¾Ð´Ñ ÑÐ¼ÐµÐ½ÑÑÐµÐ½Ð¸Ñ ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ñ ÑÐ½Ð´Ð¾ÑÐ¾ÐºÑÐ¸Ð½Ð¾Ð² Ð½Ð° ÑÑÐ°Ð´Ð¸Ð¸ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÐºÐ»ÐµÑÐ¾Ðº E. coli. ÐÐ±ÑÐµÐºÑÐ¾Ð¼ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐ²Ð»ÑÐµÑÑÑ ÑÑÐ°Ð¼Ð¼ Ð¿Ð¾Ð´Ð³ÑÑÐ¿Ð¿Ñ E. coli BL21 (DE3). ÐÑÐµÐ´Ð¼ÐµÑÐ¾Ð¼ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐ²Ð»ÑÑÑÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÐµ ÑÐ½Ð´Ð¾ÑÐ¾ÐºÑÐ¸Ð½Ñ, Ð¾Ð±ÑÐ°Ð·ÑÑÑÐ¸ÐµÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¶Ð¸Ð·Ð½ÐµÐ½Ð½Ð¾Ð³Ð¾ ÑÐ¸ÐºÐ»Ð° ÐºÐ»ÐµÑÐ¾Ðº E. coli. Ð Ð°Ð±Ð¾ÑÐ° Ð±ÑÐ»Ð° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð² ÐÐ°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ ÑÐµÐºÐ¾Ð¼Ð±Ð¸Ð½Ð°Ð½ÑÐ½ÑÑ Ð±Ð¸Ð¾ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¹ Ð² Ð¡ÐÐ±ÐÐÐÐÐ¡. ÐÑÐ²Ð¾Ð´Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹: 1) ÐÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¼Ð¸Ð½ÐµÑÐ°Ð»ÑÐ½ÑÑ ÑÑÐµÐ´ Ð´Ð»Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÐºÐ»ÐµÑÐ¾Ðº E. coli Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÐµÑ ÑÐ¼ÐµÐ½ÑÑÐ¸ÑÑ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð¾ Ð¾Ð±ÑÐ°Ð·ÑÑÑÐ¸ÑÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÑ ÑÐ½Ð´Ð¾ÑÐ¾ÐºÑÐ¸Ð½Ð¾Ð² Ð¿Ð¾ÑÐ»Ðµ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ. 2) ÐÐ·Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ ÑÐ¾Ð»ÐµÐ²Ð¾Ð³Ð¾ ÑÐ¾ÑÑÐ°Ð²Ð° ÑÑÐµÐ´ Ð½Ðµ Ð²Ð»Ð¸ÑÐµÑ Ð½Ð° ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð¾ Ð¾Ð±ÑÐ°Ð·ÑÑÑÐ¸ÑÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÑ ÑÐ½Ð´Ð¾ÑÐ¾ÐºÑÐ¸Ð½Ð¾Ð². 3) Ð£Ð¼ÐµÐ½ÑÑÐµÐ½Ð¸Ðµ ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÑ Ð¿ÑÐ¸Ð²ÐµÐ»Ð¾ Ðº ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ñ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½-Ð½Ð¾Ð¹ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð¸ ÑÐ²ÐµÐ»Ð¸ÑÐ¸Ð»Ð¾ ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ðµ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÑ ÑÐ½Ð´Ð¾ÑÐ¾ÐºÑÐ¸Ð½Ð¾Ð² Ð² Ð¿ÑÐ¾Ð±Ð°Ñ. 4) ÐÐ¿ÑÐ¸Ð¼Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð²Ð°ÑÐ¸Ð°Ð½Ñ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÐºÐ»ÐµÑÐ¾Ðº E. coli ÑÐµÐºÐ¾Ð¼ÐµÐ½Ð´ÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ðµ ÑÑÐµÐ´Ñ Ð9 Ð¿ÑÐ¸ ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÐµ 37 Â°Ð¡.
description: When writing the literature review, the following databases were used: Cyber-Leninka, EBS Aibuks.ru, elib.spbstu.ru, eLIBRARY.RU, PudMed. Statistical processing of experimental data was carried out using the software package: Microsoft Excel. Topic of the final qualification work: Cultivation of the producer strain E. coli in order to reduce the content of bacterial endotoxins. The purpose of the work: to develop a problem in working with E. coli â the formation of endotoxins and methods for reducing their concentration. Research objectives: - conduct a literature review; - choose methods for performing research; - learn how to determine endotoxins; - to find methods for reducing the content of endotoxins at the stage of cultiva-tion of E. coli cells. The object of the study is a strain of the subgroup E. coli BL21 (DE3). The subject of the study is bacterial endotoxins formed during the life cycle of E. coli cells. The work was carried out at the Laboratory of Recombinant Biotechnologies at the St. Petersburg Research Institute of Vitality. Research findings: 1) The use of mineral media for culturing E. coli cells can reduce the amount of bacterial endotoxins formed after culturing. 2) Changing the salt composition of the media does not affect the amount of bac-terial endotoxins formed. 3) Decrease in temperature led to a decrease in the concentration of the obtained biomass and increased the content of bacterial endotoxins in the samples. 4) The optimal option for culturing E. coli cells, I recommend using the M9 me-dium at a temperature of 37 Â°C.