Additional file 1 of Improved lipid productivity in Nannochloropsis gaditana in nitrogen-replete conditions by selection of pale green mutants
Creators: name: Cecchin, Michela
name: Berteotti, Silvia
name: Paltrinieri, Stefania
name: Vigliante, Ivano
name: Iadarola, Barbara
name: Giovannone, Barbara
name: Maffei, Massimo E.
name: Delledonne, Massimo
name: Ballottari, Matteo
Publisher: name: figshare
Year: 2020
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Plant Biology

description: Additional file 1: Table S1. Coverage obtained by Illumina sequencing for WT and e8 mutant strain. Table S2. Mutation identified on gene coding for proteins putatively located in the chloroplast. Prediction of chloroplast transit peptide was performed with Hectar software. Figure S1. Mutants screening by 680/730 nm absorption ratio. Absorption ratio 680/730 nm was used to assay the chlorophylls per cell content. Only colonies with a reduction of at least 25% was selected for further analyses. Error bars are reported as standard deviation (n=3). Figure S2. Light dependent zeaxanthin accumulation in WT and e8. Samples were illuminated for 1 h with a strong light (2500 μmol photons m-2 s-1). Pigments composition was evaluated at different time points by DMSO extraction and HPLC analysis. (A) Depoxidation index calculated as (zea + anthera/2)/(anthera+viola+zea). (B) Zeaxanthin per carotenoid content. (C) Zeaxanthin per chlorophyll content. Errors are reported as standard deviation, significantly different values are marked with * if p&lt;0.05 and ** if p&lt;0.01, as determined by unpaired two-sample t-test (n=3). Figure S3. Chlorophyll bleaching in wild type and e8 mutant strain exposed to strong light. Chlorophyll bleaching kinetics of WT and e8 mutant strains were determined measuring the decrease of chlorophyll absorption upon exposure to 2500 μmol photons m-2 s-1. Errors are reported as standard deviation, (n=3). The statistical analysis of the results obtained was performed by unpaired two sample t-test revealing no statistically significant difference being p-values &gt; 0.1 at the different time points. Figure S4. Biomass and lipid productivity of WT and e8 mutant at different irradiances. (A)) Maximum daily productivity in terms of gr L-1 day-1. (B, C) Nile red fluorescence of WT and e8 mutant normalized to dry weight (B) or to the culture volume (C). (D) Fold change of Nile red fluorescence and biomass dry weight on a volumetric base in e8 mutant compared to WT. Errors are reported as standard deviation, significantly different values are marked with * if p&lt;0.05 and ** if p&lt;0.01, as determined by unpaired two sample t-test (n=3). 60. Figure S5. Dry weight and FAME content in WT and e8 mutant in nitrogen starvation. Dry weight(A) and FAME content (B) in cells grown in nitrogen deplete medium for WT and e8 mutant strain. Errors are reported as standard deviation, the statistical significance of differences between WT and e8 is indicated as ** (p&lt;0.01), as determined by unpaired two-sample t-test (n=3). Figure S6. Acyl chain composition of lipid fraction from WT and e8 mutant in nitrogen replete conditions (+N) or after nitrogen starvation (-N). (A) fatty acid content per liter of culture. (B) Fold change of fatty acid fraction on total fatty acids content in e8 normalized to the WT case. Errors are reported as standard deviation, statistically significantly different values between WT and e8 in (A) and values statistically significantly different than 1 in (B) are marked with * if p&lt;0.05 and ** if p&lt;0.01, as determined by unpaired two sample t-test (n=3). Figure S7. Visible light transmittance in photobioreactors at different layers for WT and e8 mutant cultures. Figure S8. GO slim terms of mutated genes of e8. The GO terms were restricted to GO slim terms of plant for an easier visualization. Each dot is proportional with the number of genes related to a specific category of GO terms (max 30 genes, min 1 genes).