title: ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¸Ð¼Ð¼ÑÐ½Ð¾ÑÑÐ¸Ð¼ÑÐ»Ð¸ÑÑÑÑÐµÐ³Ð¾ ÑÑÑÐµÐºÑÐ° Ð¸Ð¾Ð½Ð¸Ð·Ð¸ÑÑÑÑÐµÐ³Ð¾ Ð¸Ð·Ð»ÑÑÐµÐ½Ð¸Ñ Ð¸ Ð¿ÑÐ¾ÑÐ¾Ð½Ð·Ð°Ñ Ð²Ð°ÑÐ½ÑÑ ÑÐ°Ð´Ð¸Ð¾ÑÐµÐ½ÑÐ¸Ð±Ð¸Ð»Ð¸Ð·Ð°ÑÐ¾ÑÐ¾Ð² Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ Ð±Ð¾ÑÐ° Ð´Ð»Ñ ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ñ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð¿ÑÐ¾ÑÐ¾Ð½Ð½Ð¾Ð¹ ÑÐµÑÐ°Ð¿Ð¸Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Research of the immunostimulating effect of ionizing radiation and proton-capture radiosensitizers based on boron to increase the effectiveness of proton therapy
Creators: name: ÐÑÑÑ, ÐÐ²Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: Ð Ð°Ð´Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ñ
Ð¿ÑÐ¾ÑÐ¾Ð½Ð½Ð°Ñ ÑÐµÑÐ°Ð¿Ð¸Ñ
Ð°Ð±ÑÐºÐ¾Ð¿Ð°Ð»ÑÐ½ÑÐ¹ ÑÑÑÐµÐºÑ
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description: ÐÑÐ¾ÑÐ¾Ð½Ð½Ð°Ñ ÑÐµÑÐ°Ð¿Ð¸Ñ ÑÐ²Ð»ÑÐµÑÑÑ Ð¾Ð´Ð½Ð¸Ð¼ Ð¸Ð· ÑÐ°Ð¼ÑÑ ÑÐ¾Ð²ÑÐµÐ¼ÐµÐ½Ð½ÑÑ Ð¸ ÑÑÑÐµÐºÑÐ¸Ð²Ð½ÑÑ Ð²Ð¸Ð´Ð¾Ð² Ð»ÑÑÐµÐ²Ð¾Ð¹ ÑÐµÑÐ°Ð¿Ð¸Ð¸. ÐÐ° Ð´Ð°Ð½Ð½ÑÐ¹ Ð¼Ð¾Ð¼ÐµÐ½Ñ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ñ Ð¼ÐµÐ¶Ð´ÑÐ½Ð°ÑÐ¾Ð´Ð½ÑÐµ Ð¿ÑÐ¾ÑÐ¾ÐºÐ¾Ð»Ñ Ð»ÐµÑÐµÐ½Ð¸Ñ, Ð²ÐºÐ»ÑÑÐ°ÑÑÐ¸Ðµ Ð¿ÑÐµÐ´Ð¿Ð¸ÑÐ°Ð½Ð½ÑÑ Ð´Ð¾Ð·Ñ, ÑÐµÐ¶Ð¸Ð¼ ÑÑÐ°ÐºÑÐ¸Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ, Ð¾Ð±ÑÑÐ¼Ñ Ð¾Ð±Ð»ÑÑÐµÐ½Ð¸Ñ Ð² Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾ÑÑÐ¸ Ð¾Ñ Ð³Ð¸ÑÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐ¸Ð¿Ð° Ð¾Ð¿ÑÑÐ¾Ð»Ð¸. ÐÐ´Ð½Ð°ÐºÐ¾ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾ÑÑÐ¸ ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ñ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÑÐµÑÐ°Ð¿Ð¸Ð¸ Ð¸ ÑÐ°Ð´Ð¸Ð¾ÑÑÐ²ÑÑÐ²Ð¸ÑÐµÐ»ÑÐ½Ð¾ÑÑÐ¸ Ð¾Ð¿ÑÑÐ¾Ð»ÐµÐ²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð¾ÑÑÐ°ÑÑÑÑ Ð°ÐºÑÑÐ°Ð»ÑÐ½ÑÐ¼Ð¸ Ð² Ð½Ð°ÑÑÐ¾ÑÑÐµÐµ Ð²ÑÐµÐ¼Ñ. ÐÐµÑÐ²ÑÐ¼ ÑÑÐ°Ð¿Ð¾Ð¼ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¾ Ð¾Ð±Ð»ÑÑÐµÐ½Ð¸Ðµ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð»Ð¸Ð½Ð¸Ð¹ Ð³Ð»Ð¸Ð¾Ð±Ð»Ð°ÑÑÐ¾Ð¼Ñ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° (Ð172 Ð¸ ÐÐ»-Ð¢) Ñ Ð´Ð¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸ÐµÐ¼ Ð±Ð¾ÑÐºÐ°Ð¿ÑÐ°ÑÐ° Ð¸ Ð±ÐµÐ· Ð½ÐµÐ³Ð¾ Ð½Ð° Ð¿ÑÐ¾ÑÐ¾Ð½Ð½Ð¾Ð¹ ÑÑÑÐ°Ð½Ð¾Ð²ÐºÐµ Varian ProBeam Ð´Ð¾Ð·Ð°Ð¼Ð¸ 2, 4, 6, 8, 10 Ð¸ 20 ÐÑÐµÐ¹ (Ð±ÐµÐ· ÑÑÐ°ÐºÑÐ¸Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð´Ð¾Ð·Ñ). ÐÐ¾ÑÐ»Ðµ ÑÑÐ¾Ð³Ð¾ Ð±ÑÐ»Ð¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ñ Ð¾ÑÐµÐ½ÐºÐ° ÑÐ°Ð·Ð¼ÐµÑÐ¾Ð² Ð¿Ð¾Ð¿ÑÐ»ÑÑÐ¸Ð¹ ÐºÐ»ÐµÑÐ¾Ðº, ÑÐµÐ·Ð°Ð·ÑÑÐ¸Ð½ÑÐµÑÑ Ð¸ Ð°Ð½Ð°Ð»Ð¸Ð· Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸ ÐºÐ»ÐµÑÐ¾Ðº(Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ). ÐÐ¾ÑÐ»Ðµ ÑÑÐ¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¾Ð³Ð¸ÑÐ½ÑÐ¹ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½Ñ Ð±ÑÐ» Ð¿ÑÐ¾Ð²ÐµÐ´ÑÐ½ Ð½Ð° ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ Ð»Ð¸Ð½Ð¸Ð¸ DU145, Ð¾Ð±Ð»ÑÑÑÐ½Ð½Ð¾Ð¹ Ð´Ð¾Ð·Ð°Ð¼Ð¸ 2, 4 Ð¸ 6 ÐÑÐµÐ¹. ÐÑÐ¾ÑÐ¾Ð¹ ÑÐ°ÑÑÑÑ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ» ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½Ñ in vivo Ð½Ð° Ð¼ÑÑÐ°Ñ Ñ ÑÐµÐ»ÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð¸Ð¼Ð¼ÑÐ½Ð¾ÑÑÐ¸Ð¼ÑÐ»Ð¸ÑÑÑÑÐµÐ³Ð¾ Ð¿ÑÐ¾ÑÐ¸Ð²Ð¾Ð¾Ð¿ÑÑÐ¾Ð»ÐµÐ²Ð¾Ð³Ð¾ ÑÑÑÐµÐºÑÐ° Ð²Ð²ÐµÐ´ÐµÐ½Ð¸Ñ ÐºÐ»ÐµÑÐ¾Ðº Ð¡Ð¢26, Ð¾Ð±Ð»ÑÑÑÐ½Ð½ÑÑ Ð»ÐµÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð´Ð¾Ð·Ð¾Ð¹ Ð¿ÑÐ¾ÑÐ¾Ð½Ð¾Ð² (30 ÐÑ). Ð ÑÐ°Ð¼ÐºÐ°Ñ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð±Ð¾ÑÐºÐ°Ð¿ÑÐ°ÑÐ° Ð½Ð° Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÑÑ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð¿ÑÐ¾ÑÐ¾Ð½Ð½Ð¾Ð³Ð¾ Ð¸Ð·Ð»ÑÑÐµÐ½Ð¸Ñ Ð½Ðµ Ð±ÑÐ»Ð¾ Ð²ÑÑÐ²Ð»ÐµÐ½Ð¾ ÑÐ°Ð´Ð¸Ð¾ÑÐµÐ½ÑÐ¸Ð±Ð¸Ð»Ð¸Ð·Ð¸ÑÑÑÑÐµÐ³Ð¾ ÑÑÑÐµÐºÑÐ°, Ð·Ð°ÑÐ²Ð»ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð² ÑÐ°Ð±Ð¾ÑÐµ Cirrone et al., Â«First experimental proof of Proton Boron Capture Therapy (PBCT) to enhance proton therapy effectivenessÂ», 2018. Ð ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐµ in vivo Ð±ÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾ ÑÐ¾ÑÐ¼Ð¾Ð¶ÐµÐ½Ð¸Ðµ ÑÐ¾ÑÑÐ° Ð¾Ð¿ÑÑÐ¾Ð»ÐµÐ²Ð¾Ð³Ð¾ ÑÐ·Ð»Ð° Ð¸ ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ðµ Ð²ÑÐ¶Ð¸Ð²Ð°ÐµÐ¼Ð¾ÑÑÐ¸ Ð¿ÑÐ¸ Ð¿ÑÐµÐ´Ð²Ð°ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ð¼ Ð²Ð²ÐµÐ´ÐµÐ½Ð¸Ð¸ Ð¶Ð¸Ð²Ð¾ÑÐ½ÑÐ¼ Ð¾Ð±Ð»ÑÑÑÐ½Ð½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð¿Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ Ñ Ð¶Ð¸Ð²Ð¾ÑÐ½ÑÐ¼Ð¸ Ð² ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½ÑÑ Ð³ÑÑÐ¿Ð¿Ð°Ñ. ÐÐ¾Ð¶Ð½Ð¾ Ð¿ÑÐµÐ´Ð¿Ð¾Ð»Ð¾Ð¶Ð¸ÑÑ, ÑÑÐ¾ Ð¸Ð¼Ð¼ÑÐ½Ð¾ÑÑÐ¸Ð¼ÑÐ»Ð¸ÑÑÑÑÐµÐµ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ðµ, Ð¼Ð¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ðµ Ð² Ð½Ð°ÑÑÐ¾ÑÑÐµÐ¼ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¸, Ð¸Ð¼ÐµÐµÑ ÑÑÑÐµÑÑÐ²ÐµÐ½Ð½ÑÐ¹ Ð²ÐºÐ»Ð°Ð´ Ð² ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð°Ð±ÑÐºÐ¾Ð¿Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÑÑÐµÐºÑÐ° Ð¿ÑÐ¸ Ð»ÑÑÐµÐ²Ð¾Ð¹, Ð² ÑÐ¾Ð¼ ÑÐ¸ÑÐ»Ðµ, Ð¿ÑÐ¾ÑÐ¾Ð½Ð½Ð¾Ð¹, ÑÐµÑÐ°Ð¿Ð¸Ð¸.
description: Proton therapy is one of the most effective and modern types of radiation therapy techniques. Up to date, international treatment protocols have been developed, including the prescribed dose, fractionation regimen, and irradiation volume depending on the histological type of tumor. However, the possibilities of increasing the effectiveness of therapy and radiosensitivity of tumor cells remain relevant at the present time. In this study we have irradiated with protons human glioblastoma cells (A172 and Gl-T) with and without addition of sodium borocaptate (BSH) on the Varian Probeam therapy system with a single dose of 2, 4, 6, 8, 10 and 20 Gy. The biological effect was assessed based on the resazurin test, colony count and morphology observation. After that similar studies were carried out on DU145 cell lines with radiation doses of 2, 4 and 6 Gy. The second part of the work was an in vivo experiment on mice with the aim of study the effect of the immunostimulating antitumor effect of the injection CT26 cell irradiated with a lethal dose of protons (30 Gy). As part of the study of the effect of borcaptate on the biological efficiency of proton radiation, no radiosensitizing effect was revealed, which was stated in the work Cirrone et al., Â«First experimental proof of Proton Boron Capture Therapy (PBCT) to enhance proton therapy effectivenessÂ», 2018. In an experiment in vivo, inhibition of the growth of a tumor node and an increase in survival were shown after preliminary cell injections of irradiated cells to animals in comparison with animals in control groups. It can be assumed that the immunostimulating effect modeled in the present study has a significant contribution to the formation of the abscopal effect in radiation, incl. proton therapy.