title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ NK-ÐºÐ»ÐµÑÐ¾Ðº Ð¸ Ð¸Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐ°ÑÑÐ¸Ñ Ð½Ð° Ð°ÐºÑÐ¸Ð²Ð°ÑÐ¸Ñ Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ ÑÐ¸Ð³Ð½Ð°Ð»ÑÐ½ÑÑ Ð¿ÑÑÐµÐ¹ Ð² ÐºÐ»ÐµÑÐºÐ°Ñ ÑÑÐ¾ÑÐ¾Ð±Ð»Ð°ÑÑÐ° Ð² ÑÐ¸ÑÑÐµÐ¼Ðµ in vitro
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: The impact of NK cells and their microparticles on activation of intracellular signaling pathways in trophoblast in vitro
Creators: name: ÐÑÑÑÐ³Ð¸Ð½Ð°, ÐÑÐµÐ½Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: ÑÑÐ¾ÑÐ¾Ð±Ð»Ð°ÑÑ
NK-ÐºÐ»ÐµÑÐºÐ¸
STAT
JAK/STAT ÑÐ¸Ð³Ð½Ð°Ð»ÑÐ½ÑÐ¹ Ð¿ÑÑÑ
Ð¸Ð½Ð²Ð°Ð·Ð¸Ñ
Ð¼Ð¸ÐºÑÐ¾ÑÐ°ÑÑÐ¸ÑÑ
IL-1Î²
trophoblast
NK cells
JAK/STAT signaling pathway
invasion
microparticles

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ NK-ÐºÐ»ÐµÑÐ¾Ðº Ð¸ Ð¸Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐ°ÑÑÐ¸Ñ Ð½Ð° Ð°ÐºÑÐ¸Ð²Ð°ÑÐ¸Ñ Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ ÑÐ¸Ð³Ð½Ð°Ð»ÑÐ½ÑÑ Ð¿ÑÑÐµÐ¹ Ð² ÐºÐ»ÐµÑÐºÐ°Ñ ÑÑÐ¾ÑÐ¾Ð±Ð»Ð°ÑÑÐ° Ð² ÑÐ¸ÑÑÐµÐ¼Ðµ in vitroÂ». Ð Ð°Ð±Ð¾ÑÐ° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ Ð¼ÐµÐ¶ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð²Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ð¹ Ð¤ÐÐÐÐ£ Â«ÐÐÐ Ð°ÐºÑÑÐµÑÑÑÐ²Ð°, Ð³Ð¸Ð½ÐµÐºÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¸ ÑÐµÐ¿ÑÐ¾Ð´ÑÐºÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¸Ð¼. Ð.Ð. ÐÑÑÐ°Â». Ð Ð°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¾ÑÐ¾Ð±ÐµÐ½Ð½Ð¾ÑÑÐµÐ¹ Ð°ÐºÑÐ¸Ð²Ð°ÑÐ¸Ð¸ STAT-Ð¿ÑÑÐ¸ Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐ¸Ð³Ð½Ð°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð² ÐºÐ»ÐµÑÐºÐ°Ñ ÑÑÐ¾ÑÐ¾Ð±Ð»Ð°ÑÑÐ° Ð»Ð¸Ð½Ð¸Ð¸ JEG-3 Ð² Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð¸Ñ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ñ ÐºÐ»ÐµÑÐºÐ°Ð¼Ð¸ Ð¸ Ð¼Ð¸ÐºÑÐ¾ÑÐ°ÑÑÐ¸ÑÐ°Ð¼Ð¸ Ð»Ð¸Ð½Ð¸Ð¸ NK-92. Ð Ð¿ÑÐ¾ÑÐµÑÑÐµ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¾ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¾ ÑÐ¾ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÐºÐ»ÐµÑÐ¾Ðº Ð»Ð¸Ð½Ð¸Ð¸ ÑÑÐ¾ÑÐ¾Ð±Ð»Ð°ÑÑÐ° JEG-3 Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐ°ÑÑÐ¸ÑÐ°Ð¼Ð¸ ÐºÐ»ÐµÑÐ¾Ðº Ð»Ð¸Ð½Ð¸Ð¸ NK-92 Ð² ÑÐµÑÐµÐ½Ð¸Ðµ 24 ÑÐ°ÑÐ¾Ð², Ð° ÑÐ°ÐºÐ¶Ðµ Ð´Ð¸ÑÑÐ°Ð½ÑÐ½Ð¾Ðµ ÑÐ¾ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÐºÐ»ÐµÑÐ¾Ðº Ð»Ð¸Ð½Ð¸Ð¹ JEG-3 Ð¸ NK-92 Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÑÐ¸ÑÑÐµÐ¼Ñ ÑÑÐ°Ð½ÑÐ²ÐµÐ»Ð», Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð¾Ð±ÑÐµÐ³Ð¾ Ð±ÐµÐ»ÐºÐ° Ð¿Ð¾ Ð¼ÐµÑÐ¾Ð´Ñ ÐÑÐµÐ´ÑÐ¾ÑÐ´Ð° Ð¸ Ð¸Ð¼Ð¼ÑÐ½Ð¾Ð±Ð»Ð¾ÑÑÐ¸Ð½Ð³ Ñ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸ÐµÐ¼ ÑÐ¾ÑÑÐ¾ÑÐ¸Ð»Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ Ð¸ Ð½ÐµÑÐ¾ÑÑÐ¾ÑÐ¸Ð»Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ ÑÐ¾ÑÐ¼ STAT1, STAT3 Ð¸ ERK1/2. ÐÐ½Ð°Ð»Ð¸Ð· Ð°ÐºÑÐ¸Ð²Ð°ÑÐ¸Ð¸ Ð´Ð°Ð½Ð½ÑÑ Ð±ÐµÐ»ÐºÐ¾Ð² Ð¿ÑÐµÐ´Ð¿Ð¾Ð»Ð°Ð³Ð°Ð» ÑÐ°ÑÑÑÑ Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ñ Ð¸Ð½ÑÐµÐ½ÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÑÐ¾ÑÑÐ¾ÑÐ¸Ð»Ð¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð¹ ÑÐ¾ÑÐ¼Ñ Ðº Ð½ÐµÑÐ¾ÑÑÐ¾ÑÐ¸Ð»Ð¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð¹ ÑÐ¾ÑÐ¼Ñ Ð±ÐµÐ»ÐºÐ°. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ðµ ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ñ STAT3 Ð² Ð»Ð¸Ð·Ð°ÑÐ°Ñ ÐºÐ»ÐµÑÐ¾Ðº Ð»Ð¸Ð½Ð¸Ð¸ JEG-3, Ð¿Ð¾Ð²ÑÑÐµÐ½Ð¸Ðµ ÑÐ¾Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ñ pSTAT3 (Ser727)/STAT3 Ð² ÐºÐ»ÐµÑÐºÐ°Ñ Ð»Ð¸Ð½Ð¸Ð¸ JEG-3 Ð¿Ð¾ÑÐ»Ðµ Ð¸Ñ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐ°ÑÑÐ¸ÑÐ°Ð¼Ð¸ Ð¸Ð½ÑÐ°ÐºÑÐ½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð»Ð¸Ð½Ð¸Ð¸ NK-92 Ð¸ Ð°ÐºÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ IL-1Î² ÐºÐ»ÐµÑÐ¾Ðº. ÐÐ¾ÐºÐ°Ð·Ð°Ð½Ð¾ Ð¾ÑÑÑÑÑÑÐ²Ð¸Ðµ Ð·Ð½Ð°ÑÐ¸Ð¼ÑÑ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹ ÑÑÐ¾Ð²Ð½Ñ STAT1 Ð¸ ÐµÐ³Ð¾ ÑÐ¾ÑÑÐ¾ÑÐ¸Ð»Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ ÑÐ¾ÑÐ¼ Ð² ÐºÐ»ÐµÑÐºÐ°Ñ Ð»Ð¸Ð½Ð¸Ð¸ JEG-3 Ð¿Ð¾ÑÐ»Ðµ Ð¸Ñ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐ°ÑÑÐ¸ÑÐ°Ð¼Ð¸ ÐºÐ»ÐµÑÐ¾Ðº Ð»Ð¸Ð½Ð¸Ð¸ NK-92, Ð°ÐºÑÐ¸Ð²Ð°ÑÐ¸Ð¸ ERK1/2 ÐºÐ¸Ð½Ð°Ð·Ñ ÑÐ°ÐºÐ¶Ðµ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¾ Ð½Ðµ Ð±ÑÐ»Ð¾. ÐÑÑÐ²Ð»ÐµÐ½Ð¾ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ðµ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð° ÑÐ¾ÑÑÐ¾ÑÐ¸Ð»Ð¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð¿Ð¾ ÑÐµÑÐ¸Ð½Ñ STAT3 Ð¸ ÑÐ¾Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ñ pSTAT3 (Ser727)/STAT3 Ð² ÐºÐ»ÐµÑÐºÐ°Ñ Ð»Ð¸Ð½Ð¸Ð¸ JEG-3 Ð¿ÑÐ¸ Ð´Ð¸ÑÑÐ°Ð½ÑÐ½Ð¾Ð¼ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ð¸ ÐºÐ»ÐµÑÐ¾Ðº Ð»Ð¸Ð½Ð¸Ð¹ JEG-3 Ð¸ NK-92.
description: Topic of the thesis: âThe impact of NK cells and their microparticles on activation of intracellular signaling pathways in trophoblast in vitroâ. The work was carried out at the laboratoriy of intercellular communications of D. Ott Research Institute of Obstetrics, Gynecology and Reproductology. At this work we studied activation features of JAK/STAT intracellular signaling pathway in trophoblast cells after incubation with cells and microparticles of NK-92 line. We cultured trophoblastic JEG-3 cells with microparticles of NK-92 cells (Natural killers) for 24 hours. Also, we cultured JEG-3 and NK-92 cells using a transwell system as a model of a distant co-culturing of cells. We determined a total protein by Bradford method and the Western blot was performed. We defined expression level of phosphorylated and non-phosphorylated forms of STAT1, STAT3 and ERK1 / 2. An analysis of the activation of these proteins suggested the calculation of the ratio of the intensity of the phosphorylated form to the non-phosphorylated form of the protein. A decrease of STAT3 expression after their interaction with microparticles of intact NK cells and activated NK cells by IL-1Î² is observed in lysates of JEG-3 cell lines. Ratio pSTAT3 (Ser727) / STAT3 increased in JEG-3 after they had been cultured together with microparticles of NK cells. No significant changes in the level of STAT1 and its phosphorylated forms in JEG-3 cells after their interaction with microparticles of NK-92 cells were shown. An activation of ERK1 / 2 kinase was also not detected. A decrease in serine phosphorylated STAT3 and pSTAT3 (Ser727) / STAT3 ratio in JEG-3 cells was observed after distant interaction of JEG-3 and NK-92 cells.