title: ÐÐ¾Ð²ÑÑÐµÐ½Ð¸Ðµ ÑÑÐ¾Ð²Ð½Ñ ÑÑÐ°Ð½ÑÑÐµÐºÑÐ¸Ð¸ Ð¿ÑÐ¸ Ð´Ð¾ÑÑÐ°Ð²ÐºÐµ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ð° Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ½ÑÐ¼Ð¸ Ð½Ð°Ð½Ð¾Ð½Ð¾ÑÐ¸ÑÐµÐ»ÑÐ¼Ð¸ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ½Ð¾Ð³Ð¾ ÑÐ¾ÑÑÐ°Ð²Ð°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Increasing the level of transfection of the appointment of the genetic material polymer nonductors complex composition
Creators: name: ÐÑÐºÑÐµÐµÐ²Ð°, ÐÐ½Ð°ÑÑÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: Ð³ÐµÐ½Ð½Ð°Ñ ÑÐµÑÐ°Ð¿Ð¸Ñ
Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ½ÑÐµ Ð½Ð°Ð½Ð¾ÑÐ¸ÑÐµÐ»Ð¸
Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾Ñ ÐÐÐÐ°Ð·Ñ ii
Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð´Ð½Ð°Ñ ÐÐÐ
Ð¸Ð½ÐºÑÐ±Ð°ÑÐ¸Ñ ÐºÐ»ÐµÑÐ¾Ðº
gene therapy
polymer carriers
DNAse ii inhibitor
plasmid DNA
cell incubation

description: Ð Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ Ð¾ÑÐµÐ½ÐµÐ½Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÐ¾Ð² Ð·Ð°Ð¿Ð°ÐºÐ¾Ð²ÐºÐ¸ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ð° Ð² Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ½ÑÐµ Ð½Ð°Ð½Ð¾Ð½Ð¾ÑÐ¸ÑÐµÐ»Ð¸ Ð¸ ÑÑÐ»Ð¾Ð²Ð¸Ð¹ Ð¸Ñ ÑÐ¾Ð²Ð¼ÐµÑÑÐ½Ð¾Ð¹ Ð¸Ð½ÐºÑÐ±Ð°ÑÐ¸Ð¸ Ñ ÐºÐ»ÐµÑÐºÐ°Ð¼Ð¸ Ð¼Ð»ÐµÐºÐ¾Ð¿Ð¸ÑÐ°ÑÑÐ¸Ñ Ð½Ð° ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÑÑÐ°Ð½ÑÑÐµÐºÑÐ¸Ð¸. ÐÐ»Ñ Ð´Ð¾ÑÑÐ¸Ð¶ÐµÐ½Ð¸Ñ ÑÐµÐ»Ð¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¸ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ñ Ð¿ÑÐ¾ÑÐ¾ÐºÐ¾Ð»Ñ Ð·Ð°Ð¿Ð°ÐºÐ¾Ð²ÐºÐ¸ Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð´Ð½Ð¾Ð¹ ÐÐÐ Ð² ÑÐ°ÑÑÐ¸ÑÑ ÐºÐ°ÑÐ±Ð¾Ð½Ð°ÑÐ° ÐºÐ°Ð»ÑÑÐ¸Ñ, Ð¾ÐºÑÑÐ¶ÐµÐ½Ð½ÑÐµ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ½Ð¾Ð¹ Ð¾Ð±Ð¾Ð»Ð¾ÑÐºÐ¾Ð¹. ÐÑÐ»Ð¾ Ð¸Ð·ÑÑÐµÐ½Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð¾ÑÐ³Ð°Ð½Ð¸ÑÐµÑÐºÐ¸Ñ Ð´Ð¾Ð±Ð°Ð²Ð¾Ðº (PARG, Ð¿ÐµÐ¿ÑÐ¸Ð´Ð½ÑÐ¹ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾Ñ ÐÐÐÐ°Ð·Ñ IISLRLLQWFLWAC)Ð½Ð° ÑÑÐ¾Ð²ÐµÐ½Ñ ÑÑÐ°Ð½ÑÑÐµÐºÑÐ¸Ð¸ ÐºÐ»ÐµÑÐ¾Ðº Ð¼Ð»ÐµÐºÐ¾Ð¿Ð¸ÑÐ°ÑÑÐ¸Ñ. ÐÑÐ»Ð¸ Ð¿Ð¾Ð´Ð¾Ð±ÑÐ°Ð½Ñ Ð¾Ð¿ÑÐ¸Ð¼Ð°Ð»ÑÐ½ÑÐµ ÑÑÐ»Ð¾Ð²Ð¸Ñ ÑÐ¾ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÐºÐ»ÐµÑÐ¾Ðº Ð¼Ð»ÐµÐºÐ¾Ð¿Ð¸ÑÐ°ÑÑÐ¸Ñ Ð¸ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ½ÑÑ Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸Ñ, ÑÐ¾Ð´ÐµÑÐ¶Ð°ÑÐ¸Ñ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð». Ð ÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð¼ Ð¿ÑÐ¾Ð´ÐµÐ»Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÐµÑÑÑ Ð¾ÑÑÐ°Ð±Ð¾ÑÐ°Ð½Ð½Ð°Ñ Ð¼ÐµÑÐ¾Ð´Ð¸ÐºÐ° Ð´Ð¾ÑÑÐ°Ð²ÐºÐ¸ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ð° Ð´Ð»Ñ ÑÑÐ°Ð½ÑÑÐµÐºÑÐ¸Ð¸ ÐºÐ»ÐµÑÐ¾Ðº Ð¼Ð»ÐµÐºÐ¾Ð¿Ð¸ÑÐ°ÑÑÐ¸Ñ, ÑÑÐ¾ ÑÐ²Ð»ÑÐµÑÑÑ Ð¾ÑÐ½Ð¾Ð²Ð¾Ð¹ Ð´Ð»Ñ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐ¸Ñ ÑÑÐ°Ð¿Ð¾Ð² ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ñ Ð¿ÑÐ¾ÐµÐºÑÐ°.
description: In this work, the influence of different parameters of packing of genetic material and conditions of incubation of mammalian cells on the efficiency of transfection was evaluated. To achieve the goal of the research, protocols for packing plasmid DNA into calcium carbonate particles surrounded by a polymer shell were developed. The effect of organic additives (PARG, peptide inhibitor DNase II SLRLLQWFLWAC) on the level of mammalian cell transfectionwas studied.The optimal conditions for incuba-tion of mammalian cells and polymer nanoparticles containing genetic material were selected. The result of this work is a proven method for the delivery of genetic material, applicable for transfection of mammalian cells, which is the basis for the subsequent stages of the project's development.