Additional file 1 of Comprehensive transcriptomics and metabolomics revealed the antifungal mechanism of Cymbopogon citratus essential oil nanoemulsion against Fusarium solani
Creators: name: Wen, JinRui
name: Liao, HongXin
name: Nie, HongYan
name: Ling, CuiQiong
name: Zhang, LiYan
name: Xu, FuRong
name: Dong, Xian
Publisher: name: figshare
Year: 2024
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: Developmental Biology
subject: Plant Biology

description: Additional file 1: Fig. S1. Effect of mixing Tween-80 and anhydrous ethanol at different Km values on the ternary phase diagram of the nanoemulsion. The best Km was selected by the area of the blue part. Fig. S2. NEO stability test. NEO under different (A) temperatures; (B) salt concentrations; (C) rotational speeds for 15 min; (D) effect of different pH values on the stability of NEO; (E) effects of different illuminations on nanoemulsions. The bars represent ± SD for three independent biological experiments. Fig. S3. The antifungal activity of different light intensities on NEO treatment after 48 h. (A) Inhibition growth curve of F. solani after different NEO treatments. (B) Growth to the seventh day, the opposite of F. solani. The data are expressed as the mean ± standard deviation of three biological replicates. Fig. S4. Chemical composition of the two emulsions. (A) Chemical composition of EO; (B) Chemical composition of NEO. (C) Chemical composition of TEO. The values are presented as the mean of three replicates. Fig. S5. Transcriptome analysis of F. solani treated with NEO and TEO; (A) PCA plots of differential gene expression; (B) CK vs. TEO, CK vs. NEO intercomparison of differential gene Venn diagram; Differential genetic volcano map of (C) NEO; (D) TEO. Each dot in the volcano plot denotes one gene. The green spots denote genes with downregulated differential expression; the red spots denote genes with upregulated differential expression; the gray spots denote the detected genes without significant differences. All treatments were consistent with the above. Fig. S6. GO enrichment analysis of DEGs in CK vs. NEO (A) and in CK vs. TEO (B), including biological process, cellular component, and molecular function. The horizontal axis represents the degree of enrichment, the vertical axis represents the functional classification, the bubble size represents the number of genes, and the bubble color represents the enrichment significance p value. All treatments were consistent with the above. Fig. S7. (A) Changes in related genes during meiosis. (B) Expression of ribosome-related genes. Fig. S8. Extensive targeted metabolomic analysis of F. solani treated with NEO and TEO. (A) Metabolite PCA graph; (B) histogram of up- and downregulated differential metabolites from pairwise comparisons; (C) differential metabolites upregulated in the comparison of CK vs. TEO and CK vs. NEO; (D) differential metabolites downregulated in the comparison of CK vs. TEO and CK vs. NEO. Fig. S9. The top 20 KEGG pathways enriched by DAMs. (A) DAMs of CK vs. NEO; (B) DAMs of CK vs. TEO. The X-axis is the enrichment factor, and the Y-axis represents the name of the pathway. The bubble size represents the number of DAMs involved. The bubble color indicates the enrichment degree of the pathway. All treatments were consistent with the above. Fig. S10. Amount of key metabolites: (A) Metabolites associated with cell membrane composition; (B) the amount of D-trehalose; (C) the amount of key metabolites in the network diagram. All treatments were consistent with the above. Fig. S11. A total of 9 genes were screened by qRT-PCR verification of TCA cycle and meiosis-related genes. The ratio of qPCR expression to CK expression was calculated as 1.