title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ pH ÑÐ°ÑÑÐ²Ð¾ÑÐ° Ð½Ð° ÑÑÑÑÐºÑÑÑÑ Ð¸ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð½ÑÑ ÑÐ¸Ð±ÑÐ¸Ð»Ð» Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ Ð±ÐµÑÐ°-2-Ð¼Ð¸ÐºÑÐ¾Ð³Ð»Ð¾Ð±ÑÐ»Ð¸Ð½Ð° Ð¸ Ð»Ð¸Ð·Ð¾ÑÐ¸Ð¼Ð°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: pH effect on the structure and properties beta-2-microglobulin and lysozyme amyloid fibrils
Creators: name: Ð¨ÐµÑÑÑÐ½ÑÐ², ÐÐ²Ð°Ð½
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð½ÑÐµ ÑÐ¸Ð±ÑÐ¸Ð»Ð»Ñ
Ð»Ð¸Ð·Ð¾ÑÐ¸Ð¼
Ð±ÐµÑÐ°-2-Ð¼Ð¸ÐºÑÐ¾Ð³Ð»Ð¾Ð±ÑÐ»Ð¸Ð½
Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ñ
ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½ÑÐµ Ð·Ð¾Ð½Ð´Ñ
ÑÐ¸Ð¾ÑÐ»Ð°Ð²Ð¸Ð½-Ñ
8-(Ð°Ð½Ð¸Ð»Ð¸Ð½Ð¾)-1-Ð½Ð°ÑÑÐ°Ð»Ð¸Ð½ÑÑÐ»ÑÑÐ¾Ð½Ð°Ñ
amyloid fibrils
lysozyme
beta-2-microglobulin
morphology
fluorescent probes
thioflavin t
1-anilino-8-naphthalene sulfonate

description: ÐÐ¼Ð¸Ð»Ð¾Ð¸Ð´Ð½ÑÐµ ÑÐ¸Ð±ÑÐ¸Ð»Ð»Ñ Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ñ ÑÐ°ÑÑÐ²Ð¾ÑÐ¸Ð¼ÑÐ¼Ð¸ Ð±ÐµÐ»ÐºÐ°Ð¼Ð¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐ¾Ð±Ð¸ÑÐ°ÑÑÑÑ Ð² Ð½ÐµÑÐ°ÑÑÐ²Ð¾ÑÐ¸Ð¼ÑÐµ Ð²Ð¾Ð»Ð¾ÐºÐ½Ð°, ÑÑÑÐ¾Ð¹ÑÐ¸Ð²ÑÐµ Ðº Ð´ÐµÐ³ÑÐ°Ð´Ð°ÑÐ¸Ð¸. ÐÑ Ð½Ð°ÐºÐ¾Ð¿Ð»ÐµÐ½Ð¸Ðµ Ð² ÑÐºÐ°Ð½ÑÑ Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð° ÑÐ²ÑÐ·Ð°Ð½Ð¾ Ñ ÑÑÐ´Ð¾Ð¼ Ð½ÐµÐ¸Ð·Ð»ÐµÑÐ¸Ð¼ÑÑ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ð¹, ÑÐ°ÐºÐ¸Ñ ÐºÐ°Ðº Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ ÐÐ»ÑÑÐ³ÐµÐ¹Ð¼ÐµÑÐ° Ð¸ ÐÐ°ÑÐºÐ¸Ð½ÑÐ¾Ð½Ð°, Ð³ÐµÐ¼Ð¾Ð´Ð¸Ð°Ð»Ð¸Ð·Ð½ÑÐ¹ Ð¸ Ð»Ð¸Ð·Ð¾ÑÐ¸Ð¼Ð¾Ð²ÑÐ¹ Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð¾Ð·Ñ, Ð¸ Ð´ÑÑÐ³Ð¸Ðµ. ÐÐ¼Ð¸Ð»Ð¾Ð¸Ð´Ð½ÑÐµ ÑÐ¸Ð±ÑÐ¸Ð»Ð»Ñ Ð´ÐµÐ¼Ð¾Ð½ÑÑÑÐ¸ÑÑÑÑ Ð²ÑÑÐ¾ÐºÑÑ ÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾ÑÑÑ, Ð¿Ð¾ÑÑÐ¾Ð¼Ñ Ð¿Ð¾Ð¸ÑÐº ÑÐ°ÐºÑÐ¾ÑÐ¾Ð² Ð¸ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð¾Ð² Ð½Ð°ÑÑÑÐµÐ½Ð¸Ñ ÑÑÑÑÐºÑÑÑÑ Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð½ÑÑ ÑÐ¸Ð±ÑÐ¸Ð»Ð» ÑÐ²Ð»ÑÐµÑÑÑ Ð°ÐºÑÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð·Ð°Ð´Ð°ÑÐµÐ¹. Ð­ÑÐ° ÑÐ°Ð±Ð¾ÑÐ° Ð±ÑÐ»Ð° Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð° Ð½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ ÑÐ Ð½Ð° ÑÑÑÑÐºÑÑÑÑ Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð½ÑÑ ÑÐ¸Ð±ÑÐ¸Ð»Ð» Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ Ð»Ð¸Ð·Ð¾ÑÐ¸Ð¼Ð° Ð¸ Î²-2-Ð¼Ð¸ÐºÑÐ¾Ð³Ð»Ð¾Ð±ÑÐ»Ð¸Ð½Ð°. ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»Ð¸ÑÑ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð¾Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ ÑÐ¿ÐµÐºÑÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸ Ð¸ ÑÐ»ÐµÐºÑÑÐ¾Ð½Ð½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸. Ð ÑÐ°Ð±Ð¾ÑÐµ Ð±ÑÐ»Ð¸ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ñ ÑÐ°Ð·Ð»Ð¸ÑÐ¸Ñ Ð² ÑÑÑÑÐºÑÑÑÐµ Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð½ÑÑ ÑÐ¸Ð±ÑÐ¸Ð»Ð» Ð¿ÑÐ¸ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ Ð·Ð½Ð°ÑÐµÐ½Ð¸ÑÑ pH. ÐÐ¼Ð¸Ð»Ð¾Ð¸Ð´Ð½ÑÐµ ÑÐ¸Ð±ÑÐ¸Ð»Ð»Ñ Î²-2-Ð¼Ð¸ÐºÑÐ¾Ð³Ð»Ð¾Ð±ÑÐ»Ð¸Ð½Ð° ÑÐ°Ð·ÑÑÑÐ°ÑÑÑÑ Ð² ÑÐµÐ»Ð¾ÑÐ½ÑÑ ÑÐ°ÑÑÐ²Ð¾ÑÐ°Ñ. Ð ÐºÐ¸ÑÐ»ÑÑ Ð¸ Ð½ÐµÐ¹ÑÑÐ°Ð»ÑÐ½ÑÑ ÑÐ°ÑÑÐ²Ð¾ÑÐ°Ñ (ÑÐ 1 â 9) Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð½ÑÐµ ÑÐ¸Ð±ÑÐ¸Ð»Ð»Ñ ÑÐ¾ÑÑÐ°Ð½ÑÑÑÑÑ, Ð´Ð»Ð¸Ð½Ð° Ð¸ ÑÑÐµÐ¿ÐµÐ½Ñ Ð¸Ñ ÐºÐ»Ð°ÑÑÐµÑÐ¸Ð·Ð°ÑÐ¸Ð¸, ÑÐ²ÑÐ·ÑÐ²Ð°Ð½Ð¸Ðµ Ñ ThT Ð¸ ANS, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¸Ñ Ð²ÑÐ¾ÑÐ¸ÑÐ½Ð°Ñ ÑÑÑÑÐºÑÑÑÐ° Ð·Ð°Ð²Ð¸ÑÑÑ Ð¾Ñ ÑÐ ÑÐ°ÑÑÐ²Ð¾ÑÐ°. ÐÐ¼Ð¸Ð»Ð¾Ð¸Ð´Ð½ÑÐµ ÑÐ¸Ð±ÑÐ¸Ð»Ð»Ñ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ Ð»Ð¸Ð·Ð¾ÑÐ¸Ð¼Ð° Ð¿ÑÐ¸ÑÑÑÑÑÐ²ÑÑÑ Ð² ÐºÐ¸ÑÐ»ÑÑ Ð¸ ÑÐµÐ»Ð¾ÑÐ½ÑÑ ÑÐ°ÑÑÐ²Ð¾ÑÐ°Ñ, Ð° Ð² Ð½ÐµÐ¹ÑÑÐ°Ð»ÑÐ½ÑÑ pH Ð¾Ð±ÑÐ°Ð·ÑÑÑÑÑ Ð°Ð¼Ð¾ÑÑÐ½ÑÐµ Ð°Ð³ÑÐµÐ³Ð°ÑÑ.
description: Amyloid fibrils are formed by normally soluble proteins, which assemble to form insoluble fibers that are resistant to degradation. Their accumulation in the tissues is associated with a number of incurable diseases, such as Alzheimer's and Parkinsonâs diseases, hemodialysis-associated and lysozyme amyloidosis, etc. Amyloid fibrils are remarkable stable, so the search for factors of disruption of amyloid fibril structure and mechanisms is a relevant task. This work was focused on the investigation of the pH effect on the Î²-2-microglobulin and lysozyme amyloid fibrils structure. The investigations were carried out by optical spectroscopy and electron microscopy. Differences in the structure of amyloid fibrils were detected at different pH values. Î²-2-microglobulin amyloid fibrils are destroyed in alkaline solutions. In acidic and neutral solutions (pH 1 - 9), amyloid fibrils are retained, the length and degree of their clusterization, binding with ThT and ANS, as well as their secondary structure depend on the pH of the solution. Lysozyme-based amyloid fibrils are present in acidic and alkaline solutions, and amorphous aggregates form in neutral pHs.