cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Prospects and challenges of implementing DNA metabarcoding for high-throughput insect surveillance
Creators: name: Piper, Alexander
name: Batovska, Jana
name: Cogan, Noel
name: Weiss, John
name: Cunningham, John
name: Rodoni, Brendan
name: Blacket, Mark
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: © The Author(s) 2019.
Trap-based surveillance strategies are widely used for monitoring of invasive insect species, aiming to detect newly arrived exotic taxa as well as track the population levels of established or endemic pests. Where these surveillance traps have low specificity and capture non-target endemic species in excess of the target pests, the need for extensive specimen sorting and identification creates a major diagnostic bottleneck. While the recent development of standardized molecular diagnostics has partly alleviated this requirement, the single specimen per reaction nature of these methods does not readily scale to the sheer number of insects trapped in surveillance programmes. Consequently, target lists are often restricted to a few high-priority pests, allowing unanticipated species to avoid detection and potentially establish populations. DNA metabarcoding has recently emerged as a method for conducting simultaneous, multi-species identification of complex mixed communities and may lend itself ideally to rapid diagnostics of bulk insect trap samples. Moreover, the high-throughput nature of recent sequencing platforms could enable the multiplexing of hundreds of diverse trap samples on a single flow cell, thereby providing the means to dramatically scale up insect surveillance in terms of both the quantity of traps that can be processed concurrently and number of pest species that can be targeted. In this review of the metabarcoding literature, we explore how DNA metabarcoding could be tailored to the detection of invasive insects in a surveillance context and highlight the unique technical and regulatory challenges that must be considered when implementing high-throughput sequencing technologies into sensitive diagnostic applications.
The romanesque fabric of Llandaff Cathedral
Creators: name: Thurlby, Malcolm
Publisher: name: Archaeology Data Service
Year: 2025

description: Archaeologia Cambrensis, 170, 159-191
description: This paper seeks to provide a reconstruction of Bishop Urban's Cathedral Church of Llandaff, to determine the sources for the plan and the origin of the craftsmen responsible for the work, and to suggest why the building took on the appearance it did. There is no documentation to assist on these matters. Suggestions are made on the basis of careful comparative analysis of specific motifs which indicate particularly close associations with Hereford Cathedral, the great Benedictine Abbey (now Cathedral) of St Peter at Gloucester and allied work in Gloucestershire, and the work of Roger, Bishop of Salisbury from 1102 to 1139, especially in his extension of the early Norman Sarum Cathedral, and the church and former cloister of Malmesbury Abbey.
From Extinction Voices to Extinction Silences: exploring how natural history displays can shape narratives and action against future extinctions, using a case study from Bristol Museums
Creators: name: Gladstone, Isla
Publisher: name: Zenodo
Year: 2022

description: Abstract of conference oral presentation on From Extinction Voices to Extinction Silences: exploring how natural history displays can shape narratives and action against future extinctions, using a case study from Bristol Museums by Senior Curator Natural Science Isla Gladstone of Senior Curator Natural Science Bristol Museums Bristol United Kingdom for SPNHC2022 Conference, Edinburgh. June 5-10th 2022.
Chemical and biological immobilization mechanisms of potentially toxic elements in biochar-amended soils
Creators: name: Tharanga Dissanayaka Mudiyanselage
name: Franks, Ashley
name: Jianming Xu
name: N Bolan
name: H Wang
name: Caixian Tang
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: © 2019 Taylor & Francis Group, LLC.
The application of biochars for the remediation of water and soils contaminated with potentially toxic elements (PTEs) has seen a recent growing interest. The mechanisms of chemical immobilization of PTEs with biochars in aqueous media have been well defined. However, immobilization mechanisms by which biochars interact with PTEs in soil matrix are more complex. The biological immobilization mechanisms and their interactions with PTEs in biochar-amended soils are not as well defined. This review presents an overview of factors governing interactions of biochars with PTEs as well as the synergistic effect of biochars and microorganisms in biological immobilization processes. The effectiveness of biochars in improvement of microbial immobilization of PTEs mainly depends on biochar properties, application rates, and soil environments such as organic matter content, clay type and content, pH and redox potential. Although some modified biochars appear to be better than the pristine biochars for immobilization of PTEs, their potential adverse impacts on soil microbial activity should be considered. This review highlights the most common analytical methods to discover molecular interaction mechanisms between biochar and PTEs and future research areas required for the understanding of biochar-PTE interactions in polluted soil systems.
Indigenous traumatic brain injury research: responding to recruitment challenges in the hospital environment
Creators: name: Fitts, Michelle
name: Condon, Taeha
name: Gilroy, John
name: Bird, Katrina
name: Bleakley, Erica
name: Matheson, Lauren
name: Fleming, Jennifer
name: Clough, Alan R
name: Esterman, Adrian
name: Maruff, Paul
name: Bohanna, India
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Background: Hospitals are common recruitment sites for injury and disability studies. However, the clinical and rehabilitation environment can create unique challenges for researchers to recruit participant populations. While there is growing injury and disability focused research involving Indigenous people to understand the types of services and supports required by this population to enhance their recovery experiences, there is limited knowledge of researchers' experiences implementing recruitment processes in the tertiary hospital environment. This paper reflects on the specific challenges of recruiting Indigenous patients following a traumatic brain injury from two tertiary hospitals in Northern Australia. Methods: Between July 2016 and April 2018, research staff recruited eligible patients from one hospital in Queensland and one hospital in the Northern Territory. Qualitative records summarising research staff contact with patients, family members and clinical hospital staff were documented. These qualitative records, in addition to field trip notes and researcher reflections were reviewed to summarise the main challenges in gaining access to patients who fit the eligibility criteria. Results: During the recruitment process, there were five main challenges encountered: (1) Patients discharging against medical advice from hospital; (2) Discharge prior to formal emergence from Post Traumatic Amnesia as per the Westmead Post Trauma Amnesia Scale; (3) Patients under adult guardianship orders; (4) Narrow participant eligibility criteria and (5) Coordinating around patient commitments and treatment. Details of how the recruitment processes were modified throughout the recruitment phase of the study to ensure greater access to patients that met the criteria are described. Conclusion: Based on our recruitment experiences, several recommendations are proposed for future TBI studies with Indigenous Australians. In addition to treatment, Indigenous TBI patients have wide range of needs that must be addressed while in hospital. Patient engagement and data collection processes should be flexible to respond to patient needs and the hospital environment. Employment of a centralized recruiter at each hospital site may help to minimise the challenges researchers need to navigate in the hospital environment. To improve recruitment processes in hospitals, it is essential for researchers examining other health or injury outcomes to describe their recruitment experiences.
ROLE OF INTERMOLECULAR INTERACTIONS IN LINEAR FURANOCOUMARIN DERIVATIVES
Creators: name: Elahi, Ahsan
name: Kant, Rajni
Publisher: name: European Chemical Bulletin
Year: 2015

description: Energy calculations have been performed on a series of ten molecules of linear furanocoumarin derivatives to obtain the total stabilization energy i.e. lattice energy of the crystal and also the energy of neighbouring molecular pairs participating in the stabilization of the crystal. The total stabilization energy (lattice energy) lies in the range -16 to -40 kcal mol-1. Analysis of the energetics of the neighbouring molecular pairs in these structures shows the presence of different intermolecular interactions participating in the crystal packing. In addition to the significance of coulombic nature of bifurcated C-H…O hydrogen bonds, the stabilizing role of π…π stacking interactions has been realized in these structures. A careful observation of energetics of different molecular pairs involving C-H…O hydrogen bond reveals that motifs involving C(sp2)-H…O hydrogen bonds are more stabilized than those of C(sp3)-H…O.
description: European Chemical Bulletin, Vol 4, No 7-9 (2015): European Chemical Bulletin
The Great Gatehouse at Harlech Castle
Creators: name: Ashbee, Jeremy
Publisher: name: Archaeology Data Service
Year: 2025

description: Archaeologia Cambrensis, 170, 193-229
description: The great gatehouse at Harlech Castle, Merioneth, is celebrated for its imposing external appearance and for the sophistication of the defences around its ground-level entrance. Although ruined since the seventeenth century, and having lost several architectural details, the building has undergone remarkably few changes since its construction in the late thirteenth century. Its design was eclectic but also innovative, incorporating features that, if installed as planned, would have caused difficulty for those occupying or using the upper rooms. Some of these design problems were eliminated in later gatehouses elsewhere, whose designs drew on that of Harlech. Through analysis of this and other buildings in the castle and elsewhere, it is argued that the gatehouse was designed and occasionally used for the simultaneous residence of two important households. Its rooms are likely to have hosted several kings of England and Prince Owain Glyndur of Wales.
The stone font at Llandygwydd: a brief biography
Creators: name: Palmer, Tim
Publisher: name: Archaeology Data Service
Year: 2025

description: Archaeologia Cambrensis, 170, 231-235
description: The late medieval font at Llandygwydd has been exposed to the open air since the church which formerly housed it was taken down at the beginning of the twenty-first century. It is made of two English Jurassic limestones, Dundry stone and Painswick stone. The markedly differential weathering of these two limestones over the last two decades illuminates the font's history of construction, ornamentation, and repair.
Further thoughts on Ewloe Castle
Creators: name: Owen Jones, Craig
Publisher: name: Archaeology Data Service
Year: 2025

description: Archaeologia Cambrensis, 170, 237-250
description: Recent studies have called into question the long-held notion that Ewloe castle was erected by Llywelyn ap Gruffudd in and after 1257. The following article endorses Brodie's theory (2015) that the building work carried on in 1257 amounted to nothing more than a refurbishment. However, where Brodie based this theory on the idea that Ewloe was not designed to hold against a 'serious assault' a contention that is open to question other warrants can be secured for it. The likelihood is that Llywelyn ap Gruffudd had neither the financial resources nor the manpower to erect Ewloe in the period between his capture of it in late 1257 and November 1259, at which time a conference between high-ranking Welsh and English officials was held there.
2-Morpholinoethanesulfonic acid catalyzed one pot synthesis of isoindolo [2,1-a]quinazoline at room temperature under ultrasonication
Creators: name: Bhagat, D. S.
name: Katariya, M. V.
name: Ingle, R. D.
name: Joshi, V. M.
name: Bachhav, M. R.
name: Pawar, R. P.
Publisher: name: European Chemical Bulletin
Year: 2015

description: An efficient and greener one pot method has been developed for the synthesis of isoindolo [2,1- a]quinazolines using 2-morpholinoethanesulfonic acid as a water soluble greener catalyst at ambient  temperature and excellent yield of product. The synergetic effect of 2-morpholine ethane sulphonic acid and ultrasound irradiation process has been also discussed.
description: European Chemical Bulletin, Vol 4, No 7-9 (2015): European Chemical Bulletin
Mapping 'Flemish' settlements in South Wales: electromagnetic induction (EMI) survey of the villages of Wiston (Pembrokeshire) and Whitson (Monmouthshire)
Creators: name: Verbrugghe, Gerben
Publisher: name: Archaeology Data Service
Year: 2025

description: Archaeologia Cambrensis, 170, 251-274
description: Around 1112, a Fleming called Wizo settled in Wiston, a village in mid-Pembrokeshire. Together with other Flemings, he is believed to have influenced the morphology of settlements in South Wales. An in-depth morphological and metrical comparison with similar settlements in the medieval County of Flanders has not yet been attempted however. In order to allow such a study, a large-scale frequency domain multi-receiver EMI survey has been conducted in Wiston (Pembrokeshire) and Whitson (Monmouthshire), mapping former field-boundaries. This paper presents the results of the geophysical survey and first interpretation of the data in order to reconstruct these settlements' morphologies.
Carreg Cennen Castle Cave, Llandeilo, Carmarthenshire: a review of the work undertaken by T. C. Cantrill in 1900
Creators: name: Hodkinson, Poppy
name: A Walker, Elizabeth
name: M Chapman, Evan
name: Chivall, David
name: Faillace, Katie
name: Madgwick, Richard
name: J ORegan, Hannah
Publisher: name: Archaeology Data Service
Year: 2025

description: Archaeologia Cambrensis, 170, 67-76
description: Excavations by T. C. Cantrill within Carreg Cennen Castle Cave in 1900 revealed stalagmite deposits, together with human remains and a perforated horse tooth, which like a number of more recent discoveries of human remains have remained undated. Cantrill's account of his excavations and the surviving human and animal remains from the site are published in full for the first time. The horse tooth, possibly a bead or pendant, and previously suggested to be of Late Glacial age, has been radiocarbon dated to the later Roman to early medieval period and is discussed in the light of other Roman finds from the vicinity and in cave deposits in Wales and elsewhere.
Corticosteroid injection for plantar heel pain: a systematic review and meta-analysis
Creators: name: Whittaker, Glen
name: Munteanu, Shannon
name: Menz, Hylton
name: Bonanno, Daniel
name: Gerrard, James
name: Landorf, Karl
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: © 2019 The Author(s). Background: Corticosteroid injection is frequently used for plantar heel pain (plantar fasciitis), although there is limited high-quality evidence to support this treatment. Therefore, this study reviewed randomised trials to estimate the effectiveness of corticosteroid injection for plantar heel pain. Methods: A systematic review and meta-analysis of randomised trials that compared corticosteroid injection to any comparator. Primary outcomes were pain and function, categorised as short (0 to 6 weeks), medium (7 to 12 weeks) or longer term (13 to 52 weeks). Results: A total of 47 trials (2989 participants) were included. For reducing pain in the short term, corticosteroid injection was more effective than autologous blood injection (SMD -0.56; 95% CI, - 0.86 to - 0.26) and foot orthoses (SMD -0.91; 95% CI, - 1.69 to - 0.13). There were no significant findings in the medium term. In the longer term, corticosteroid injection was less effective than dry needling (SMD 1.45; 95% CI, 0.70 to 2.19) and platelet-rich plasma injection (SMD 0.61; 95% CI, 0.16 to 1.06). Notably, corticosteroid injection was found to have similar effectiveness to placebo injection for reducing pain in the short (SMD -0.98; 95% CI, - 2.06, 0.11) and medium terms (SMD -0.86; 95% CI, - 1.90 to 0.19). For improving function, corticosteroid injection was more effective than physical therapy in the short term (SMD -0.69; 95% CI, - 1.31 to - 0.07). When trials considered to have high risk of bias were excluded, there were no significant findings. Conclusions: Based on the findings of this review, corticosteroid injection is more effective than some comparators for the reduction of pain and the improvement of function in people with plantar heel pain. However, corticosteroid injection is not more effective than placebo injection for reducing pain or improving function. Further trials that are of low risk of bias will strengthen this evidence. Registration: PROSPERO registration number CRD42016053216.
Immune reconstruction effectiveness of combination antiretroviral therapy for HIV-1 CRF01_AE cluster 1 and 2 infected individuals
Creators: name: Li, Kang
name: Chen, Huanhuan
name: Li, Jianjun
name: Feng, Yi
name: Lan, Guanghua
name: Liang, Shujia
name: Liu, Meiliang
name: Rashid, Abdur
name: Xing, Hui
name: Shen, Zhiyong
name: Shao, Yiming
Publisher: name: Taylor & Francis
Year: 2021
Subjects: subject: Medicine
subject: Environmental Sciences not elsewhere classified
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Science Policy
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Virology
subject: Computational Biology

description: There are great disparities of the results in immune reconstruction (IR) of the HIV-1 infected patients during combined antiretroviral therapy (cART), due to both host polymorphisms and viral genetic subtypes. Identifying these factors and elucidating their impact on the IR could help to improve the efficacy. To study the factors influencing the IR, we conducted a 15-year retrospective cohort study of HIV-1 infected individuals under cART. The trend of CD4+ count changes was evaluated by the generalized estimating equations. Cox proportional model and propensity score matching were used to identify variables that affect the possibility of achieving IR. The tropism characteristics of virus were compared using the coreceptor binding model. In addition to baseline CD4+ counts and age implications, CRF01_AE cluster 1 was associated with a poorer probability of achieving IR than infection with cluster 2 (aHR, 1.39; 95%CI, 1.02-1.90) and other subtypes (aHR, 1.83; 95%CI, 1.31-2.56). The mean time from cART initiation to achieve IR was much longer in patients infected by CRF01_AE cluster 1 than other subtypes/sub-clusters (P < 0.001). In-depth analysis indicated that a higher proportion of CXCR4 viruses were found in CRF01_AE clusters 1 and 2 (P < 0.05), and showed tendency to favour CXCR4 binding to V3 signatures. This study indicated the immune restoration impairment found in patients were associated with HIV-1 CRF01_AE cluster 1, which was attributed to the high proportion of CXCR4-tropic viruses. To improve the effectiveness of cART, more efforts should be made in the early identification of HIV-1 subtype/sub-cluster and monitoring of virus phenotypes.
Ffos y Mynach, St David's: an ancient boundary and its purpose
Creators: name: Vyner, Blaise E
Publisher: name: Archaeology Data Service
Year: 2025

description: Archaeologia Cambrensis, 170, 77-110
description: An enigmatic earthwork boundary cutting off the western tip of St David's Head, Pembrokeshire, was first reported in 1719 with the suggestion that it might have been monastic in origin. The existence and general course of the boundary was confirmed by the Pembrokeshire antiquarian Richard Fenton some 90 years later. Exploration was renewed in 1816, when a more convoluted course for the earthwork was proposed. In the mid-nineteenth century Ffos y mynach ('the monk's dyke') was confirmed by eminent historians of St David's and its putative course thereafter shown on Ordnance Survey's maps. However, review in the mid-twentieth century led to it being left off OS maps and thereafter the boundary lost credibility to the extent that it now finds no mention in the County History, or, indeed, in most archaeological or topographic publications. This paper reviews the documentary, place-name and archaeological evidence for Ffos y mynach, concluding that the boundary does exist and that traces of it can still be found in the landscape of St David's. Having reached this conclusion, a potential date and context for the earthwork is discussed.
Significantly Elevated FMR1 mRNA and Mosaicism for Methylated Premutation and Full Mutation Alleles in Two Brothers with Autism Features Referred for Fragile X Testing
Creators: name: Field, M
name: Dudding-Byth, T
name: Arpone, M
name: Baker, Emma
name: Aliaga, SM
name: Rogers, C
name: Hickerton, C
name: Francis, D
name: Phelan, DG
name: Palmer, EE
name: Amor, DJ
name: Slater, H
name: Bretherton, L
name: Ling, L
name: Godler, DE
Publisher: name: La Trobe
Year: 2022
Subjects: classificationCode: 31
subject: Biological sciences
subject: FOS: Biological sciences
classificationCode: 3101
subject: Biochemistry and cell biology
classificationCode: 3105
subject: Genetics
classificationCode: 3107
subject: Microbiology
classificationCode: 3404
subject: Medicinal and biomolecular chemistry

description: Although fragile X syndrome (FXS) is caused by a hypermethylated full mutation (FM) expansion with ≥200 cytosine-guanine-guanine (CGG) repeats, and a decrease in FMR1 mRNA and its protein (FMRP), incomplete silencing has been associated with more severe autism features in FXS males. This study reports on brothers (B1 and B2), aged 5 and 2 years, with autistic features and language delay, but a higher non-verbal IQ in comparison to typical FXS. CGG sizing using AmplideX PCR only identified premutation (PM: 55–199 CGGs) alleles in blood. Similarly, follow-up in B1 only revealed PM alleles in saliva and skin fibroblasts; whereas, an FM expansion was detected in both saliva and buccal DNA of B2. While Southern blot analysis of blood detected an unmethylated FM, methylation analysis with a more sensitive methodology showed that B1 had partially methylated PM alleles in blood and fibroblasts, which were completely unmethylated in buccal and saliva cells. In contrast, B2 was partially methylated in all tested tissues. Moreover, both brothers had FMR1 mRNA ~5 fold higher values than those of controls, FXS and PM cohorts. In conclusion, the presence of unmethylated FM and/or PM in both brothers may lead to an overexpression of toxic expanded mRNA in some cells, which may contribute to neurodevelopmental problems, including elevated autism features.
Barapsabbas (Bar-ḥad-bešabba/Kyriakos?), a monastic founder of Mu'Allaḳ
Creators: name: Admin, CSLA
Publisher: name: University of Oxford
Year: 2016
Subjects: History

description: All evidence related to Barapsabbas (Bar-ḥad-bešabba/Kyriakos?), a monastic founder of Mu'Allaḳ.
The role of Alzheimer’s disease related factors on blood-brain barrier transport of fatty acids and astrocyte release of apolipoprotein E isoforms
Creators: name: Supti, Showmika Tabassum
Publisher: name: Monash University
Year: 2024
Subjects: classificationCode: 321499
subject: Pharmacology and pharmaceutical sciences not elsewhere classified

description: Alzheimer's disease (AD) is linked to the imbalance of iron and an essential fatty acid called docosahexaenoic acid (DHA) in the brain. A variant of a lipid-associated protein called apolipoprotein E4 increases AD risk and affects DHA movement into the brain. Iron impacts other proteins at the interface between the blood and the brain. This research examined how excess iron, apolipoprotein E status and DHA movement at the interface can contribute to AD. Findings suggest that targeting iron could be a promising therapeutic strategy for AD, as it impacts multiple factors that are important for the development of AD.
THREE-COMPONENT SYNTHESIS OF 1-ARYL-1,2,3,4-TETRAHYDROPYRIMIDO[1,2-A][1,3,5]TRIAZINE-6-ONES
Creators: name: Potapov, Andrey Yurievich
name: Shikhaliev, Khidmet Safarovich
name: Falaleev, Alexander Vladimirovich
Publisher: name: European Chemical Bulletin
Year: 2015

description: This study is concerned with the three-component method of synthesis of new 1,2,3,4-Tetrahydro-6Н-pyrimido[1,2-а][1,3,5]triazin-6-ones based on 2-amino-3H-pyrimidine-4-ones, aliphatic amines and formaldehyde. Conditions of reaction were optimized.
description: European Chemical Bulletin, Vol 4, No 7-9 (2015): European Chemical Bulletin
Corn-drying kilns in Wales: a review of the evidence
Creators: name: Comeau, Rhiannon
name: Burrow, Steve
Publisher: name: Archaeology Data Service
Year: 2025

description: Archaeologia Cambrensis, 170, 111-149
description: Corn-drying kilns are becoming increasingly recognised on archaeological sites in Wales, the charred grain and seeds they contain providing valuable evidence of dating, agricultural practices and economic activity. These multi-functional features were used for different stages of crop processing as well as for malting grain for ale-making, and their presence alone, on early sites at least, is suggestive of processes associated with social complexity. This paper examines the form, dating, function and location of recorded Welsh examples dating from later prehistory up to the sixteenth century AD and considers priorities for future research. A gazetteer lists all known examples and is published in conjunction with an online dataset which provides greater detail and is intended to be updated as new information becomes available.
INTERACTION OF 1,2-DIAMINOBENZIMIDAZOLE WITH N-ARYLIMIDES
Creators: name: Vandyshev, Dmitrii Yurievich
name: Shikhaliev, Khidmet Safarovich
name: Potapov Mail, Andrey Yurevich
Publisher: name: European Chemical Bulletin
Year: 2015

description: Substituted 10-amino-2,3,4,10-tetrahydro-4-oxo-N-arylpyrimido[1,2-a]benzimidazole-2-carboxamides are formed by condensation of 1,2-diaminobenzimidazoles with N-arylmaleimides in isopropyl alcohol in the presence of catalytic amount of acetic acid.
description: European Chemical Bulletin, Vol 4, No 7-9 (2015): European Chemical Bulletin
A probable monastic cemetery at Cwmhir Abbey
Creators: name: W Jones, Nigel
name: Driver, Toby
Publisher: name: Archaeology Data Service
Year: 2025

description: Archaeologia Cambrensis, 170, 151-157
description: Cropmarks recorded by aerial photography during the dry summer of 2018 suggested the location of an inhumation cemetery on higher ground to the north-west of the Cistercian Cwmhir Abbey, Powys. This was confirmed by trial excavation in 2019 which revealed the partial plan of eight graves, one of which was fully excavated. No dating evidence was recovered apart from unstratified sherds of medieval pottery but the location of the cemetery within the assumed extent of the monastic precinct suggests that it represents part of a cemetery associated with the abbey.
Smac mimetics LCL161 and GDC-0152 inhibit osteosarcoma growth and metastasis in mice
Creators: name: Shekhar, Tanmay
name: Burvenich, Ingrid
name: Harris, Michael
name: Rigopoulos, Angela
name: Zanker, Damien
name: Spurling, Alex
name: Parker, Belinda
name: Walkley, CR
name: Scott, Andrew
name: Hawkins, Christine
Publisher: name: La Trobe
Year: 2024
Subjects: classificationCode: 32
subject: Biomedical and clinical sciences
classificationCode: 3211
subject: Oncology and carcinogenesis
classificationCode: 4202
subject: Epidemiology

description: Background: Current therapies fail to cure over a third of osteosarcoma patients and around three quarters of those with metastatic disease. "Smac mimetics" (also known as "IAP antagonists") are a new class of anti-cancer agents. Previous work revealed that cells from murine osteosarcomas were efficiently sensitized by physiologically achievable concentrations of some Smac mimetics (including GDC-0152 and LCL161) to killing by the inflammatory cytokine TNFα in vitro, but survived exposure to Smac mimetics as sole agents. Methods: Nude mice were subcutaneously or intramuscularly implanted with luciferase-expressing murine 1029H or human KRIB osteosarcoma cells. The impacts of treatment with GDC-0152, LCL161 and/or doxorubicin were assessed by caliper measurements, bioluminescence, 18FDG-PET and MRI imaging, and by weighing resected tumors at the experimental endpoint. Metastatic burden was examined by quantitative PCR, through amplification of a region of the luciferase gene from lung DNA. ATP levels in treated and untreated osteosarcoma cells were compared to assess in vitro sensitivity. Immunophenotyping of cells within treated and untreated tumors was performed by flow cytometry, and TNFα levels in blood and tumors were measured using cytokine bead arrays. Results: Treatment with GDC-0152 or LCL161 suppressed the growth of subcutaneously or intramuscularly implanted osteosarcomas. In both models, co-treatment with doxorubicin and Smac mimetics impeded average osteosarcoma growth to a greater extent than either drug alone, although these differences were not statistically significant. Co-treatments were also more toxic. Co-treatment with LCL161 and doxorubicin was particularly effective in the KRIB intramuscular model, impeding primary tumor growth and delaying or preventing metastasis. Although the Smac mimetics were effective in vivo, in vitro they only efficiently killed osteosarcoma cells when TNFα was supplied. Implanted tumors contained high levels of TNFα, produced by infiltrating immune cells. Spontaneous osteosarcomas that arose in genetically-engineered immunocompetent mice also contained abundant TNFα. Conclusions: These data imply that Smac mimetics can cooperate with TNFα secreted by tumor-associated immune cells to kill osteosarcoma cells in vivo. Smac mimetics may therefore benefit osteosarcoma patients whose tumors contain Smac mimetic-responsive cancer cells and TNFα-producing infiltrating cells.
SYNTHESIS AND REACTIONS OF SOME NEW BENZIMIDAZOLE DERIVATIVES
Creators: name: Fikry, Reda
name: Ismail, Nabila A.
name: Said, Said A
name: Hafez, Mohammed E.
Publisher: name: European Chemical Bulletin
Year: 2015

description: 4-Amino-5-(1H-benzimidazol-2-yl)-6-(4-chlorophenyl)pyridine-3-carbonitrile 3 was obtained from reaction of 2-cyanomethyl-1H-benzimidazole 1 with chlorobenzaldehyde followed by reaction with malononitrile.  Reaction of 3 with cyclohexanone, formic acid and hydrazine hydrate afforded tetrahydrobenzonaphthyridine amine, pyrido[4,3-d]pyrimidin-4(3H)one and pyrazolo[4,3-c]pyridine-3-amine 4-6 respectively. Heterocyclization of 3 with carbon disulfide and benzoyl isothiocyante gave the corresponding pyrido[3,4-d]pyrimidindithione and thioxopyrido[4,3-d]pyrimidine methanone 7 and 8.  While, the reaction of 3 with active methylene group named ethyl cyanoacetate, diethyl malonate and with nitrous acid afforded  oxo-1,6-naphthyridin-3-carbonitrile,  carboxylate and pyrido[4,3-d][1,2,3]triazine 9-11 respectively. Dihydroimidazol pyridin-4-amine 12 was obtained from reaction of 3 with ethylendiamine and carbon disulfide.  Finally, treatment of 3 with triethyl orthoformate underwent cyclization in the presence of hydrazine hydrate afforded pyrido[4,3-d]pyrimidin-3-ylamine 14.
description: European Chemical Bulletin, Vol 4, No 7-9 (2015): European Chemical Bulletin
Smac mimetics LCL161 and GDC-0152 inhibit osteosarcoma growth and metastasis in mice
Creators: name: Shekhar, Tanmay
name: Burvenich, Ingrid JG
name: Harris, Michael
name: Rigopoulos, Angela
name: Zanker, Damien
name: Spurling, Alex
name: Parker, Belinda
name: Walkley, CR
name: Scott, Andrew
name: Hawkins, Christine
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Background: Current therapies fail to cure over a third of osteosarcoma patients and around three quarters of those with metastatic disease. "Smac mimetics" (also known as "IAP antagonists") are a new class of anti-cancer agents. Previous work revealed that cells from murine osteosarcomas were efficiently sensitized by physiologically achievable concentrations of some Smac mimetics (including GDC-0152 and LCL161) to killing by the inflammatory cytokine TNFα in vitro, but survived exposure to Smac mimetics as sole agents. Methods: Nude mice were subcutaneously or intramuscularly implanted with luciferase-expressing murine 1029H or human KRIB osteosarcoma cells. The impacts of treatment with GDC-0152, LCL161 and/or doxorubicin were assessed by caliper measurements, bioluminescence, 18FDG-PET and MRI imaging, and by weighing resected tumors at the experimental endpoint. Metastatic burden was examined by quantitative PCR, through amplification of a region of the luciferase gene from lung DNA. ATP levels in treated and untreated osteosarcoma cells were compared to assess in vitro sensitivity. Immunophenotyping of cells within treated and untreated tumors was performed by flow cytometry, and TNFα levels in blood and tumors were measured using cytokine bead arrays. Results: Treatment with GDC-0152 or LCL161 suppressed the growth of subcutaneously or intramuscularly implanted osteosarcomas. In both models, co-treatment with doxorubicin and Smac mimetics impeded average osteosarcoma growth to a greater extent than either drug alone, although these differences were not statistically significant. Co-treatments were also more toxic. Co-treatment with LCL161 and doxorubicin was particularly effective in the KRIB intramuscular model, impeding primary tumor growth and delaying or preventing metastasis. Although the Smac mimetics were effective in vivo, in vitro they only efficiently killed osteosarcoma cells when TNFα was supplied. Implanted tumors contained high levels of TNFα, produced by infiltrating immune cells. Spontaneous osteosarcomas that arose in genetically-engineered immunocompetent mice also contained abundant TNFα. Conclusions: These data imply that Smac mimetics can cooperate with TNFα secreted by tumor-associated immune cells to kill osteosarcoma cells in vivo. Smac mimetics may therefore benefit osteosarcoma patients whose tumors contain Smac mimetic-responsive cancer cells and TNFα-producing infiltrating cells.
title: Ranunculus :
title: Guidelines for the Conduct of Tests for Distinctness, Uniformity and Stability.
titleType: Subtitle
Creators: name: International Union For The Protection Of New Varieties Of Plants,
Publisher: name: International Union for the Protection of New Varieties of Plants,
Year: 0
Subjects: Plants
New Varieties of Plants
Garden Ranunculus

description: The purpose of these guidelines (“Test Guidelines”) is to elaborate the principles contained in the General Introduction (document TG/1/3), and its associated TGP documents, into detailed practical guidance for the harmonized examination of distinctness, uniformity and stability (DUS) and, in particular, to identify appropriate characteristics for the examination of DUS and production of harmonized variety descriptions.
sj-docx-1-mcr-10.1177_1077558720968365 – Supplemental material for Are Patient Experience and Outcomes for Home Health Agencies Related?
Creators: name: Schwartz, Margot L.
name: Mroz, Tracy M.
name: Thomas, Kali S.
Publisher: name: SAGE Journals
Year: 2020
Subjects: subject: 111799 Public Health and Health Services not elsewhere classified
subject: FOS: Health sciences
subject: 160807 Sociological Methodology and Research Methods
subject: FOS: Sociology

description: Supplemental material, sj-docx-1-mcr-10.1177_1077558720968365 for Are Patient Experience and Outcomes for Home Health Agencies Related? by Margot L. Schwartz, Tracy M. Mroz and Kali S. Thomas in Medical Care Research and Review
SYNTHESIS OF NEW MERCAPTOPYRIMIDINES AND THIENOPYRIMIDINES
Creators: name: Saddik, Abdelreheem Abdelfatah
Publisher: name: European Chemical Bulletin
Year: 2015

description: 2-Alkylmercapto- 4-Chloro-6-phenyl-pyrimidine-5-carbonitrile 4a-c were synthesized and converted into 2-alkylmercapto 4-mercapto-6-phenyl-pyrimidine-5-carbonitriles 7a-c. Compound 7a-b was alkylated with halogenated compounds to afford compounds 8a-g. The produced compounds underwent Thorpe-Ziegler Cyclization to give thienopyrimidines 9a-g. 5-Amino-2-alkylmercapto-4-phenyl-thieno-[2,3-d]pyrimidine-6-carboxamide derivatives 9a-f underwent cyclization  reaction using triethyl orthoformate to afford pyrimido-thienopyrimidines 10a-e.
description: European Chemical Bulletin, Vol 4, No 7-9 (2015): European Chemical Bulletin
Immune reconstruction effectiveness of combination antiretroviral therapy for HIV-1 CRF01_AE cluster 1 and 2 infected individuals
Creators: name: Li, Kang
name: Chen, Huanhuan
name: Li, Jianjun
name: Feng, Yi
name: Lan, Guanghua
name: Liang, Shujia
name: Liu, Meiliang
name: Rashid, Abdur
name: Xing, Hui
name: Shen, Zhiyong
name: Shao, Yiming
Publisher: name: Taylor & Francis
Year: 2021
Subjects: subject: Medicine
subject: Environmental Sciences not elsewhere classified
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Science Policy
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Virology
subject: Computational Biology

description: There are great disparities of the results in immune reconstruction (IR) of the HIV-1 infected patients during combined antiretroviral therapy (cART), due to both host polymorphisms and viral genetic subtypes. Identifying these factors and elucidating their impact on the IR could help to improve the efficacy. To study the factors influencing the IR, we conducted a 15-year retrospective cohort study of HIV-1 infected individuals under cART. The trend of CD4+ count changes was evaluated by the generalized estimating equations. Cox proportional model and propensity score matching were used to identify variables that affect the possibility of achieving IR. The tropism characteristics of virus were compared using the coreceptor binding model. In addition to baseline CD4+ counts and age implications, CRF01_AE cluster 1 was associated with a poorer probability of achieving IR than infection with cluster 2 (aHR, 1.39; 95%CI, 1.02-1.90) and other subtypes (aHR, 1.83; 95%CI, 1.31-2.56). The mean time from cART initiation to achieve IR was much longer in patients infected by CRF01_AE cluster 1 than other subtypes/sub-clusters (P<0.001). In-depth analysis indicated that a higher proportion of CXCR4 viruses were found in CRF01_AE clusters 1 and 2 (P<0.05), and showed tendency to favor CXCR4 binding to V3 signatures. This study indicated the immune restoration impairment found in patients were associated with HIV-1 CRF01_AE cluster 1, which was attributed to the high proportion of CXCR4-tropic viruses. To improve the effectiveness of cART, more efforts should be made in the early identification of HIV-1 subtype/sub-cluster and monitoring of virus phenotypes.
sj-pdf-1-eex-10.1177_2515127420969957 - Supplemental material for Developing Educational Escape Rooms for Experiential Entrepreneurship Education
Creators: name: Martina, Richard A.
name: Göksen, Sultan
Publisher: name: SAGE Journals
Year: 2020
Subjects: subject: 150310 Organisation and Management Theory
subject: FOS: Economics and business

description: Supplemental material, sj-pdf-1-eex-10.1177_2515127420969957 for Developing Educational Escape Rooms for Experiential Entrepreneurship Education by Richard A. Martina and Sultan Göksen in Entrepreneurship Education and Pedagogy
Patterns of drinking in Aboriginal and Torres Strait Islander peoples as self-reported on the Grog Survey App: a stratified sample
Creators: name: Lee, Kylie
name: Conigrave, James
name: Wilson, Scott
name: Perry, Christopher
name: Hayman, Noel
name: Zheng, Catherine
name: Ansari, Mustafa Al
name: Doyle, Michael
name: Room, Robin
name: Callinan, Sarah
name: Chikritzhs, Tanya
name: Slade, Tim
name: Conigrave, Katherine M
Publisher: name: La Trobe
Year: 2024
Subjects: classificationCode: 42
subject: Health sciences
subject: FOS: Health sciences
classificationCode: 4203
subject: Health services and systems

description: BACKGROUND: The Grog Survey App is a visual and interactive tablet computer-based survey application. It has been shown to be an accurate and acceptable tool to help Indigenous Australians describe what they drink. METHODS: The Grog Survey App was used to enquire into patterns of drinking in a stratified sample of Indigenous Australians in urban and remote/regional sites during testing of the App. The App asked about the last four drinking occasions in the past 12 months, including preferred alcohol types and containers; and symptoms of alcohol dependence, based on ICD-11 descriptions. Drinking patterns are presented here using medians and interquartile ranges, and the thresholds set out by the Australian National and Health and Medical Research Council guidelines. Patterns of consumption are compared by gender and remoteness, using Wilcoxon rank-sum test to compare medians. Logistic regressions tested whether alcohol types and drinking containers varied by remoteness. RESULTS: In this stratified sample most people either consumed nothing (21.7%), or consumed quantities which placed them at short- (95.6%) or long-term risk (47.8%) of harms. Drinkers in remote areas were more likely to drink beer, but less likely to drink pre-mixed spirits. 'Stubbies' and other beer glasses were popular in urban areas, compared with 'slabs' (cases of beer) in remote/regional areas. The use of improvised containers (i.e. empty juice bottles) did not vary by remoteness. Nearly one in six (15%) current drinkers reported experiencing at least two symptoms of alcohol dependence at least monthly. Average drinks per day was the consumption measure most highly correlated with each dependence symptom (r = 0.34-0.38). CONCLUSIONS: The App was able to capture a wide range of preferred alcohol types and containers, and demonstrate a diversity in how alcohol is consumed. This detail was captured in a relative brief survey delivered using an interactive and appealing tablet computer-based application.
From pyrite decay to amber breakage: the power of qualified storage and conservation to preserve and disseminate cultural and scientific heritage
Creators: name: Muchagata, João
Publisher: name: Zenodo
Year: 2022

description: Abstract of conference poster on From pyrite decay to amber breakage: the power of qualified storage and conservation to preserve and disseminate cultural and scientific heritage by João Muchagata of Museu De História Natural E Da Ciência Da Universidade Do Porto (mhnc-up) for SPNHC2022 Conference, Edinburgh. June 5-10th 2022.
Supplemental Material, Appendix_R1 - Examining the link between social capital, knowledge quality, SMEs innovativeness and performance
Creators: name: Boateng, Henry
name: Ramanah Visnupriyan
name: Kwame Simpe Ofori
name: Hinson, Robert Ebo
Publisher: name: SAGE Journals
Year: 2020
Subjects: subject: 89999 Information and Computing Sciences not elsewhere classified
subject: FOS: Computer and information sciences
subject: 150310 Organisation and Management Theory
subject: FOS: Economics and business

description: Supplemental Material, Appendix_R1 for Examining the link between social capital, knowledge quality, SMEs innovativeness and performance by Henry Boateng, Ramanah Visnupriyan, Kwame Simpe Ofori and Robert Ebo Hinson in Business Information Review
title: International capital flows and development: financial openness matters
Creators: name: Reinhardt, Dennis
name: Ricci, Luca Antonio
name: Tressel, Thierry
Publisher: name: Graduate Institute of International and Development Studies
publisherIdentifier: https://ror.org/007ygn379
publisherIdentifierScheme: ROR
Year: 2012

description: Graduate Institute of International and Development Studies Working Paper ; no. 11/2012
Additional file 1 of Host metabolome and faecal microbiome shows potential interactions impacted by age and weaning times in calves
Creators: name: Amin, Nida
name: Schwarzkopf, Sarah
name: Tröscher-Mußotter, Johanna
name: Camarinha-Silva, Amélia
name: Dänicke, Sven
name: Huber, Korinna
name: Frahm, Jana
name: Seifert, Jana
Publisher: name: figshare
Year: 2024
Subjects: subject: Biochemistry
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Physiology
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Infectious Diseases
subject: FOS: Health sciences

description: Additional file 1. Fig. S1 Age- and weaning-dependent changes in the faecal bacterial compositional profiles of calves. a Changes among alpha-diversity index (Shannon index). abcde Groups that share superscript letters are not significantly different (p > 0.05; Dunn’s post-hoc test). Standard deviations are indicated by error bars. b Significantly different bacterial phyla. ***Phyla with p < 0.001 (age × weaning effect; Kruskal–Wallis test) are shown. Fig. S2. Microbial functional predictions using KEGG pathways and the CowPI database. EarlyC/lateC log2(FC) shows differences in level-3 KEGG microbial pathways between d42, d70 and d98 earlyC (blue) and lateC (red) calves. Only metabolic pathways with relative abundance (> 1%) in at least 50% of the animals and FDR adjusted p < 0.05 (Kruskal–Wallis test) are shown. Fig. S3. Calculation of kynurenine/tryptophan ration at d70 and d98 for early weaned calves (E) and late weaned calves (L). Fig. S4. Metabolic pathway analysis based on significantly different plasma metabolites of weaning groups. Circle size indicates pathway impact and colours (yellow to red) show different levels of significance.