title: ÐÐ¾Ð´ÐµÐ»Ñ ÐºÐ¸Ð½ÐµÑÐ¸ÐºÐ¸ ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð³Ð¾ ÑÐ¸Ð³Ð½Ð°Ð»Ð° Ð¸Ð½ÑÐµÑÐºÐ°Ð»ÑÑÐ¸Ð¾Ð½Ð½Ð¾Ð³Ð¾ ÐºÑÐ°ÑÐ¸ÑÐµÐ»Ñ Ð² Ñ Ð¾Ð´Ðµ Ð°Ð¼Ð¿Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ ÐÐÐ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Kinetics Model for the Formation of a Fluorescent Signal of an Intercalation Dye during DNA Amplification
Creators: name: ÐÐ±ÑÐ°Ð³Ð¸Ð¼Ð¾Ð², ÐÐ°Ð½Ð¸Ð¸Ð»Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Ð¼Ð¾Ð´ÐµÐ»Ñ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð½Ð¾Ð¹ ÑÐµÐ¿Ð½Ð¾Ð¹ ÑÐµÐ°ÐºÑÐ¸Ð¸
ÐºÐ¸Ð½ÐµÑÐ¸ÑÐµÑÐºÐ°Ñ ÐºÑÐ¸Ð²Ð°Ñ
ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½ÑÐ¹ ÑÐ¸Ð³Ð½Ð°Ð»
Ð¸Ð½ÑÐµÑÐºÐ°Ð»ÑÑÐ¸Ð¾Ð½Ð½ÑÐ¹ ÐºÑÐ°ÑÐ¸ÑÐµÐ»Ñ
polymerase chain reaction model
kinetic curve
fluorescent signal
intercalating dye

description: ÐÐ±ÑÐµÐºÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼ ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð³Ð¾ ÑÐ¸Ð³Ð½Ð°Ð»Ð° Ð¸Ð½ÑÐµÑÐºÐ°Ð»ÑÑÐ¸Ð¾Ð½Ð½ÑÐ¼ ÐºÑÐ°ÑÐ¸ÑÐµÐ»ÐµÐ¼ (ÐÐ) Ð² ÑÐ¾Ð´Ðµ Ð°Ð¼Ð¿Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ ÐÐÐ. Ð¦ÐµÐ»ÑÑ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÐµÑÑÑ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð¼Ð°ÑÐµÐ¼Ð°ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð´Ð»Ñ Ð¾Ð¿Ð¸ÑÐ°Ð½Ð¸Ñ ÐºÐ¸Ð½ÐµÑÐ¸ÐºÐ¸ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð³Ð¾ ÑÐ¸Ð³Ð½Ð°Ð»Ð° ÐÐ Ð² Ð¿ÑÐ¾ÑÐµÑÑÐµ Ð¿ÑÐ¾ÑÐµÐºÐ°Ð½Ð¸Ñ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð½Ð¾Ð¹ ÑÐµÐ¿Ð½Ð¾Ð¹ ÑÐµÐ°ÐºÑÐ¸Ð¸ (ÐÐ¦Ð ). Ð ÑÐ°Ð±Ð¾ÑÐµ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ ÐºÐ¸Ð½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ð¹ ÑÐµÐ°ÐºÑÐ¸Ð¹ Ð±ÑÐ»Ð° Ð¼Ð°ÑÐµÐ¼Ð°ÑÐ¸ÑÐµÑÐºÐ¸ Ð¾Ð¿Ð¸ÑÐ°Ð½Ð° Ð°Ð¼Ð¿Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ ÐÐÐ. Ð Ð°ÑÑÐ¼Ð¾ÑÑÐµÐ½Ð¾ Ð´Ð²Ð° ÑÐ¿Ð¾ÑÐ¾Ð±Ð° Ð¿ÐµÑÐµÑÑÐµÑÐ° Ð½Ð°ÐºÐ¾Ð¿Ð»ÐµÐ½Ð½Ð¾Ð¹ Ð² ÑÐ¾Ð´Ðµ ÑÐµÐ°ÐºÑÐ¸Ð¸ ÐÐÐ Ð² Ð¸Ð½ÑÐµÐ½ÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð³Ð¾ ÑÐ¸Ð³Ð½Ð°Ð»Ð° Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ ÑÐ°Ð²Ð½Ð¾Ð²ÐµÑÐ¸Ñ Ð´Ð»Ñ ÑÐ²ÑÐ·ÑÐ²Ð°Ð½Ð¸Ñ Ð¼Ð¾Ð»ÐµÐºÑÐ»Ñ ÐÐ Ñ ÐÐÐ. ÐÑÐ»Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ñ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÐµ ÐºÑÐ¸Ð²ÑÐµ Ð½Ð°ÐºÐ¾Ð¿Ð»ÐµÐ½Ð¸Ñ ÐÐÐ Ð¸ ÑÐ¸Ð³Ð½Ð°Ð»Ð° Ð¸Ð½ÑÐµÑÐºÐ°Ð»ÑÑÐ¸Ð¾Ð½Ð½Ð¾Ð³Ð¾ ÐºÑÐ°ÑÐ¸ÑÐµÐ»Ñ. ÐÑÑÑÐ¼ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ ÑÐ°ÑÑÑÐ¸ÑÐ°Ð½Ð½ÑÑ Ð¸ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð±ÑÐ»Ð° Ð¿ÑÐ¾Ð´ÐµÐ¼Ð¾Ð½ÑÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð° Ð°Ð´ÐµÐºÐ²Ð°ÑÐ½Ð¾ÑÑÑ Ð¿ÑÐµÐ´Ð»Ð¾Ð¶ÐµÐ½Ð½Ð¾Ð¹ Ð¼Ð¾Ð´ÐµÐ»Ð¸. ÐÐ¾Ð´ÐµÐ»Ñ Ð±ÑÐ»Ð° ÑÐµÐ°Ð»Ð¸Ð·Ð¾Ð²Ð°Ð½Ð° Ð² Ð²Ð¸Ð´Ðµ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ñ Ð² ÑÑÐµÐ´Ðµ MATLAB.
description: The object of study is the formation of a fluorescent signal by an intercalation dye (ID) during DNA amplification. The aim of this work is to develop a mathematical model for describing the kinetics of the IR fluorescent signal during the polymerase chain reaction (PCR) model. In this work, based on the kinetic equations of reactions, DNA amplification was mathematically described. Two methods for converting the DNA accumulated during the reaction into the intensity of the fluorescent signal are considered using the equilibrium equation for binding the IC molecule to DNA. Experimental curves of DNA accumulation and intercalation dye signal were obtained. By comparing the calculated and experimental data, the adequacy of the proposed model was demonstrated. The model was implemented as a program in the MATLAB environment.