Trichoderma bombaxalis Jing Z. Sun & H. W. Liu 2022, sp. nov.
Creators: name: Yu, Shuang
name: Wang, Rui
name: Liu, Hong-Wei
name: Zhang, Xue
name: Sun, Jing-Zu
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Fungi
Ascomycota
Sordariomycetes
Hypocreales
Hypocreaceae
Trichoderma
Trichoderma bombaxalis

description: Trichoderma bombaxalis Jing Z. Sun & H.W. Liu, sp. nov. (FIGURE 2)   
Index Fungorum number: IF559555  
 Etymology: —“ bombaxalis ” (Latin), bombax+alis, meaning like mature fruits of kapok on culture media.   
 Type: — CHINA. Yinchuan: Ningxia Hui Autonomous Region, 105°97’E, 38°28’N, from rhizosphere soil of Lycium barbarum, 10 May 2021, Jing Z. Sun (HMAS 351590, holotype), ex-type culture CGMCC3.20851. GenBank accessions: ITS = OM109651, OM109652, TEF-1α = OM142599, OM142600, RPB2 = OM142601, OM142601.   
 Description: —On CMD after 72 h, colony diameter 21–25 mm at 25 °C, and 20–37 mm at 30 °C. After 7 days, mycelium loose, a layer of aerial mycelium covering the entire plate at 25 °C and 30 °C (FIGURE. 2C, G). Many conidiophores formed by 5 days, unknown aromatic odour, and no pigment.   
On PDA after 72 h, colony diameter 6–7 mm at 25 °C, and 4–5 mm at 30 °C. At 25 °C, white colony, cotton-like, dense hyphae. At 30 ℃, white flocculent colony, uniform mycelial density, coconut aroma, and no pigment. After 7 days, colony diameter 13–14 mm at 25 °C (FIGURE 2A), and 9–10 mm at 30 °C, not producing concentric rings or radial rays of mycelium (FIGURE 2E).  
 On SNA after 72 h, colony diameter 15–22 mm at 25 °C, and 17–21 mm at 30 °C. White colony, mycelium loose, light coconut aroma. After 7 days, colony edge was irregular, and mycelium was loose, coconut aroma and no pigment (FIGURE 2B, F). Conidial production noted after 5 days. Conidiophores branched verticillium-like, phialides subulate, variable in length, in whorls or opposite, 2–3(–4) branches, (8.8–)11–18(–23.2) × (1.3–)2.0–3.0(–3.4) μm (x = 14.3 × 2.4 μm, n = 20) (FIGURE 2D, H, I, J). Conidia obovate to ellipsoidal, roughened (FIGURE 2K, L), (2.0–)2.5–3.6(–4.5) × (1.0–)1.4–2.0(–2.4) µm (x = 3.1 × 1.7 µm, n = 50), length/width ratio (1.2–)1.5–1.8 (–2.4) (x = 1.6, n = 50). No sexual morphogenesis.   
 Additional specimen examined: — CHINA. Yinchuan: Ningxia Hui Autonomous Region, 105°97’E, 38°28’N, from rhizosphere soil of Lycium barbarum, 10 May 2021, Jing Z. Sun (HMAS 351591), culture CGMCC3.20852.   
 Teleomorph: —Undetermined.   
 Notes: — Trichoderma bombaxalis differs from other species in section Hypocreanum (T. austriacum, T. eucorticioides, T. sulphureum, T. subsulphureum, T. avictoriense) in the size of phialides and conidia (TABLE 2).   
 Ability to degrade cellulose and hemicellulose   
 Trichoderma bombaxalis was inoculated on xylan medium and CMC-Na medium for 7 days (FIGURE 3). Transparent zones were present around the colony on CMC-Na medium following Congo red dyeing and decolouration (FIGURE 3D), indicating a capacity to hydrolyze cellulose. However, no transparent zones were observed on the xylan medium, indicating weak or no ability to use hemicellulose. Trichoderma bombaxalis may be a potential candidate for cellulase exploration.
description: Published as part of Yu, Shuang, Wang, Rui, Liu, Hong-Wei, Zhang, Xue & Sun, Jing-Zu, 2022, Trichoderma bombaxalis sp. nov., isolated from rhizosphere soils of Lycium barbarum, pp. 147-157 in Phytotaxa 547 (2) on pages 152-155, DOI: 10.11646/phytotaxa.547.2.2, http://zenodo.org/record/6571419