cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Rethinking gendered anti-Muslim racism in a relational matrix of race and gender
Creators: name: Khandoker, Nasrin
name: Kuric, Dermana
name: CARR, JAMES
Publisher: name: University of Limerick
Year: 2024
Subjects: classificationCode: 4410
subject: Sociology
subject: FOS: Sociology

description: Gendered ideas of Muslims and Islam are one of the central organising principles of anti-Muslim racism. However, discussions around gendered anti-Muslim racism often ignore ‘Muslim men’ as a gendered category despite ideas about ‘Muslim women’ being constructed in direct relation to (often implicitly assumed presence of) their male counterparts. As problematised by Alimahomed-Wilson (2020), historical anti-Muslim racist representations of Muslim men as violent and oppressive have not been often considered in these gendered perspectives, but instead treated as a ‘gender-neutral’ facet of anti-Muslim racism. Developing on previous works, our research findings highlight that anti-Muslim racism is inherently gendered, not only because women are disproportionately targeted in Islamophobic attacks but because of its gendered essence. Informed by feminist scholars, our understanding of gender is a relational matrix between men and women upon which we elaborate this gendered foundation of anti-Muslim racism. Anti-Muslim racist ideologies are premised on myths and stereotypical ideas which claim that: 1) Islam is an inherently misogynistic religion; 2) ‘Muslim men’ are therefore inherently violent and oppressive, primarily towards Muslim women, but also backwards and unable to govern themselves or their communities and, as such, are in need of correction or control (Abu-Lughod, 2013; Farris, 2017; Kumar, 2012; inter alia). In this article, building on the previous analyses of the gendered aspect of anti-Muslim racism and our research findings based on fieldwork with Muslim communities in Ireland (n = 193), we argue that anti-Muslim racism is gendered not only because it affects women more, but also because: 1) its underlying gendered racial ideology constructs Muslim women as the passive Other of assumed ‘violent Muslim masculinity’; 2) ‘Western liberal’ anti-Muslim discourses, claiming to be vying for the liberation of all women, assume it as their ‘duty’ to save ‘Muslim women’; 3) with ‘white feminism’ often providing the moral ground for this saviour image, ignoring Muslim women's emancipatory agency and politics.
Northern Community Maps - Estimating thermal energy loads in remote and northern communities to facilitate a net-zero transition
Creators: name: Maynard, Ian
name: Abdulla, Ahmed
Publisher: name: Zenodo
Year: 2022
Subjects: remote communities

description: The northern community maps found and used for "A method for estimating thermal energy loads in Canada’s remote and northern communities" by Ian Maynard and Ahmed Abdulla. This zip folder contains publicly available base maps and planning and zoning maps for 40 remote communities in Canada's north. Please see supporting data file named "Supporting Information - A method for estimating thermal energy loads in Canada's remote and northern communities" for indication how each map is used.
Glutamate and substance P coexist in primary afferent terminals in the superficial laminae of spinal cord.
Creators: name: De Biasi, S.
name: Rustioni, A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1988

description: By light microscopic immunocytochemistry it has been previously shown that approximately equal to 70% of the neurons in rat dorsal root ganglia are labeled with an antiserum for glutamate conjugated to hemocyanin; the smaller among these neurons are also positive for substance P. By using a postembedding ImmunoGold method and electron microscopy, it is shown here that synaptic terminals in the superficial laminae of the spinal cord of rats selectively stain for the same glutamate antiserum. Immunolabeling is in small dome-shaped and in large scalloped synaptic terminals. Scalloped terminals are of two types. One type consists of dark terminals with many agranular vesicles of different size and a few large granular vesicles; these are probably endings of unmyelinated and small myelinated primary afferent fibers. The other type consists of light terminals with small agranular vesicles homogeneous in size with neurofilaments and many mitochondria; these are probably endings of larger myelinated primary afferent fibers. By means of double-labeling electron microscopic immunocytochemistry with colloidal gold particles of two different sizes, it is also shown here that substance P is present in only the dark type of glutamate-labeled scalloped terminals. The primary afferent origin of the terminals labeled by the antisera for glutamate and for substance P is demonstrated by a triple-labeling strategy: immunocytochemistry for both antisera on sections from rats in which dorsal rhizotomy or dorsal root ganglion injection of horseradish peroxidase conjugated to wheat germ agglutinin was performed. It is proposed that glutamate is the neurotransmitter in primary afferents mediating input from different peripheral receptor classes, including nociceptors. Effects of glutamate and substance P on spinal dorsal horn neurons may result from co-release of these two mediators from the same dorsal root afferent terminal.
High-level expression of a bioengineered, cysteine-free hepatocyte-stimulating factor (interleukin 6)-like protein.
Creators: name: Bishop, G. A.
name: Stebbins, J. R.
name: Fowlkes, D. M.
name: Snouwaert, J. N.
name: Frelinger, J. A.
name: Jambou, R. C.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1988

description: Hepatocyte-stimulating factor, interferon-beta 2, B-cell stimulation factor 2, and hybridoma/plasmacytoma growth factor are identical proteins presently referred to as interleukin 6 (IL-6). Through the use of synthetic oligonucleotide technology, we have constructed a biologically active recombinant IL-6 (rIL-6) gene based on the sequence of a human IL-6 cDNA. The synthetic gene encodes a cysteine-free, bioengineered rIL-6 protein that is expressed at high levels in Escherichia coli as a tripartite fusion protein. Cleavage of the fusion protein with collagenase releases a 23-kDa rIL-6 protein that can be easily purified to homogeneity. We show that the rIL-6 protein displays a range of biological activities similar to those of natural human IL-6, as demonstrated by its ability to (i) protect cells from viral infection, (ii) stimulate the synthesis of fibrinogen in rat FAZA 967 cells, and (iii) induce the terminal differentiation of B cells, resulting in elevated secretion of immunoglobulin.
Identification of an immunodominant antigenic site involving the capsid protein VP3 of hepatitis A virus.
Creators: name: Lemon, S. M.
name: Jansen, R. W.
name: Cohen, J. I.
name: Stapleton, J. T.
name: Ping, L. H.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1988

description: Hepatitis A virus, an hepatotropic picornavirus, is a common cause of acute hepatitis in man for which there is no available vaccine. Competitive binding studies carried out in solid phase suggest that neutralizing monoclonal antibodies to hepatitis A virus recognize a limited number of epitopes on the capsid surface, although the polypeptide locations of these epitopes are not well defined. Neutralization-escape mutants, selected for resistance to monoclonal antibodies, demonstrate broad cross-resistance to other monoclonal antibodies. Sequencing of virion RNA from several of these mutants demonstrated that replacement of aspartic acid residue 70 of capsid protein VP3 (residue 3070) with histidine or alanine confers resistance to neutralization by monoclonal antibody K2-4F2 and prevents binding of this antibody and other antibodies with similar solid-phase competition profiles. These results indicate that residue 3070 contributes to an immunodominant antigenic site. Mutation at residue 102 of VP1 (residue 1102) confers partial resistance against antibody B5-B3 and several other antibodies but does not prevent antibody attachment. Both VP3 and VP1 sites align closely in the linear peptide sequences with sites of neutralization-escape mutations in poliovirus and human rhinovirus, suggesting conservation of structure among these diverse picornaviruses. However, because partial neutralization resistance to several monoclonal antibodies (2D2, 3E1, and B5-B3) was associated with mutation at either residue 3070 or residue 1102, these sites appear more closely related functionally in hepatitis A virus than in these other picornaviruses.
Initial Definition of the 5GMeta Architecture
Creators: name: RAISSI FATMA
name: AMADOU KOUNTCHE Djibrilla
name: BREVI Daniele
name: BONETTO Edoardo
name: PACELLA Francesca
name: MARTIN Angel
name: AMENDOLA Danilo
name: SANCHEZ Sergio
name: SERON Mikel
name: RIZZI Gianluca
name: HAMZA-CHERIF Arslane
name: BOUJELBEN Maissa
name: ELHADED Mohamed
name: CASTAÑEDA AGUARDO Oscar Agustin
name: FRENI Pierluigi
name: NACIRI Mehdi
Publisher: name: Zenodo
Year: 2021

description: The purpose of the D2.3 is twofold. Its first goal is mainly to provide an architecture capable of addressing the set of requirements necessary to deploy different innovative use cases based on vehicular data monetisation among which the demonstrators that will be carried out within the 5GMETA project. This architecture, in its first iteration, is designed to incorporate several cutting edge 5G features and cloud-hosted services that will ensure the data monetisation objective of the project. The second aim of the deliverable is to specify open application interfaces dedicated to Third Party, through which they can access the available 5GMETA platform services.
Isolation of cDNAs of scrapie-modulated RNAs by subtractive hybridization of a cDNA library.
Creators: name: Duguid, J. R.
name: Seed, B.
name: Rohwer, R. G.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1988

description: We have developed a subtractive cloning procedure based on the hybridization of single-stranded cDNA libraries constructed in pi H3M, a vector containing the phage M13 origin of replication. We have used this strategy to isolate three transcripts whose abundance is increased in scrapie-infected brain. DNA sequence analysis showed that they represent glial fibrillary acidic protein, metallothionein II, and the B chain of alpha-crystallin; the latter two may represent a response to stress.
Purified secB protein of Escherichia coli retards folding and promotes membrane translocation of the maltose-binding protein in vitro.
Creators: name: Weiss, J. B.
name: Bassford, P. J.
name: Ray, P. H.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1988

description: The efficient export of a subset of Escherichia coli envelope proteins is dependent upon the product of the secB gene. Previous studies indicated that SecB promotes the export of the periplasmic maltose-binding protein (MBP) by preventing premature folding of the precursor MBP in the cytoplasm into an export-incompetent form. In this study, SecB has been purified to homogeneity and shown to be a soluble, cytoplasmic, multimeric protein composed of identical 17-kDa subunits. SecB was required for efficient in vitro translocation of MBP into inverted membrane vesicles. The addition of purified SecB to an in vitro system prepared from SecB- cells significantly enhanced MBP translocation. The purified protein also quantitatively retarded folding of precursor MBP into a stable, protease-resistant conformation in the absence of membranes. Finally, the inclusion of excess purified SecB in a SecB+ in vitro system significantly prolonged the time in which precursor MBP remained competent for posttranslational import into membrane vesicles.
Asteroseismic inference on the spin-orbit misalignment and stellar parameters of HAT-P-7
Creators: name: Lund, Mikkel N.
name: Lundkvist, Mia
name: Aguirre, Victor Silva
name: Houdek, Günter
name: Casagrande, Luca
name: Van Eylen, Vincent
name: Campante, Tiago L.
name: Karoff, Christoffer
name: Kjeldsen, Hans
name: Albrecht, Simon
name: Chaplin, William J.
name: Nielsen, Martin Bo
name: Degroote, Pieter
name: Davies, Guy R.
name: Handberg, Rasmus
Publisher: name: arXiv
Year: 2014
Subjects: subject: Earth and Planetary Astrophysics (astro-ph.EP)
subject: Solar and Stellar Astrophysics (astro-ph.SR)
subject: FOS: Physical sciences

description: The measurement of obliquities in star-planet systems is of great importance for the understanding of planet system formation and evolution. The bright and well studied HAT-P-7 system is intriguing as several Rossiter-McLaughlin (RM) measurements found a large projected obliquity in this system, but it was so far not possible to determine if the orbit is polar and/or retrograde. The goal of this study is to measure the stellar inclination and hereby the full 3D obliquity of the HAT-P-7 system instead of only the 2D projection as measured by the RM effect. In addition we provide an updated set of stellar parameters for the star. We use the full set of available observations from Kepler spanning Q0-Q17 to produce the power spectrum of HAT-P-7. We extract oscillation mode frequencies via an MCMC peak-bagging routine, and use the results from this to estimate the stellar inclination angle. Combining this with the projected obliquity from RM and the inclination of the orbital plane allows us to determine the stellar obliquity. We use asteroseismology to model the star from the extracted frequencies using two different approaches to the modelling where either the MESA or the GARSTEC stellar evolution codes are adopted. Using our updated asteroseismic modelling we find, i.a., the following stellar parameters for HAT-P-7: M=1.51{+0.04}{-0.05}Msun, $R=2.00{+0.01}{-0.02}Rsun, and age = 2.07{+0.28}{-0.23} Gyr. Our asteroseismic modelling offers a high precision on the stellar parameters, for instance is the uncertainty on age of the order ~11%. For the stellar inclination we estimate i_star<36.5 deg., which translates to an obliquity between 83 and 111 deg. We find that the planet HAT-P-7b is likely retrograde in its orbit, and that the orbit is close to being polar. The new parameters for the star gives an updated planetary density of 0.65+-0.03 g cm^{-3}, which is lower than previous estimates.
description: 18 pages, 12 figures, Accepted for publication in Astronomy and Astrophysics (A&A)
The Norma Arm Region Chandra Survey: X-ray Populations in the Spiral Arms
Creators: name: Fornasini, Francesca M.
name: Tomsick, John A.
name: Bodaghee, Arash
name: Krivonos, Roman A.
name: An, Hongjun
name: Rahoui, Farid
name: Gotthelf, Eric V.
name: Bauer, Franz E.
name: Stern, Daniel
Publisher: name: arXiv
Year: 2014
Subjects: subject: High Energy Astrophysical Phenomena (astro-ph.HE)
subject: FOS: Physical sciences

description: We present a catalog of 1415 X-ray sources identified in the Norma arm region Chandra survey (NARCS), which covers a 2 deg x 0.8 deg region in the direction of the Norma spiral arm to a depth of $\approx$20 ks. Of these sources, 1130 are point-like sources detected with $\geq3σ$ confidence in at least one of three energy bands (0.5-10, 0.5-2, and 2-10 keV), five have extended emission, and the remainder are detected at low significance. Since most sources have too few counts to permit individual classification, they are divided into five spectral groups defined by their quantile properties. We analyze stacked spectra of X-ray sources within each group, in conjunction with their fluxes, variability, and infrared counterparts, to identify the dominant populations in our survey. We find that $\sim$50% of our sources are foreground sources located within 1-2 kpc, which is consistent with expectations from previous surveys. Approximately 20% of sources are likely located in the proximity of the Scutum-Crux and near Norma arm, while 30% are more distant, in the proximity of the far Norma arm or beyond. We argue that a mixture of magnetic and nonmagnetic CVs dominates the Scutum-Crux and near Norma arms, while intermediate polars (IPs) and high-mass stars (isolated or in binaries) dominate the far Norma arm. We also present the cumulative number count distribution for sources in our survey that are detected in the hard energy band. A population of very hard sources in the vicinity of the far Norma arm and active galactic nuclei dominate the hard X-ray emission down to $f_X\approx10^{-14}$ erg cm$^{-2}$ s$^{-1}$, but the distribution curve flattens at fainter fluxes. We find good agreement between the observed distribution and predictions based on other surveys.
description: 27 pages, 19 figures, 12 tables, catalog will be made available in published ApJ version and by personal request
Regulation of the ecdysteroid titer of Manduca sexta: reappraisal of the role of the prothoracic glands.
Creators: name: Nakanishi, K.
name: Lee, S. S.
name: Sakurai, S.
name: Gilbert, L. I.
name: Warren, J. T.
name: Rountree, D. B.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1988

description: It is generally accepted that the prothoracic glands of insects produce ecdysone, which is converted by a 20-monooxygenase in peripheral tissues to the major molting hormone, 20-hydroxyecdysone. Incubation in vitro of the prothoracic glands of larval or pupal Manduca sexta in the presence of a hemolymph protein fraction (HPF) increased the ecdysteroid content of the medium almost 8-fold. A comparable increase was noted when HPF was added to medium preconditioned with prothoracic glands but from which the glands had been removed. We used a differential RIA to show that a major product of the prothoracic glands in vitro cross-reacts with antiserum (20-hydroxyecdysone-2-succinylthyroglobulin amide; H-2) that retains affinity to ecdysteroids having a modified A ring. However, this product did not bind to antiserum (ecdysone-22-succinylthyroglobulin amide; H-22) that has affinity mainly for ecdysteroids modified at the side chain. We employed radiolabeled precursor studies with prothoracic glands in vitro and a combination of analytical techniques (NMR, CD, MS) to demonstrate that the major ecdysteroid release from the glands is a mixture of 2-dehydroecdysone and 3-dehydroecdysone (1:2), which is rapidly reduced to ecdysone in the presence of HPF. We postulate that the active component of HPF is 3 beta 3 beta (2 beta)-formin-3(2)-ketoecdysteroid reductase. These results may explain several anomalous observations pertaining to the molting of insect fragments in the absence of prothoracic glands and suggest a complex system for the control of insect molting and metamorphosis.
Multivariate clinical phenotype - brain structure association in autoimmune limbic encephalitis
Creators: name: Maleki Balajoo, Somayeh
name: Elben, Saskia
name: Nicolaisen, Eliana
name: Räuber, Saskia
name: Conradi, Nadine
name: Siebenbrodt, Kai
name: Schwarz, Michael
name: Podewils, Viola von
name: Gorny, Iris
name: Hahn, Wiebke
name: Kerkhoff, Ruth
name: Caspers, Julian
name: Turowski, Bernd
name: Neuhaus, Elisabeth
name: Hattingen, Elke
name: Dörfler, Arnd
name: Schembs, Leah
name: Kemmling, Andre
name: Aktas, Orhan
name: Ringelstein, Marius
name: Zöllner, Johann Philipp
name: Willems, Laurent
name: Gollwitzer, Stephanie
name: Podewils, Felix von
name: Belke, Markus
name: Knake, Susanne
name: Floel, Agnes
name: Hamer, Hajo
name: Strzelczyk, Adam
name: Rosenow, Felix
name: Meuth, Sven G.
name: Eickhoff, Simon
name: Melzer, Nico
name: GENON, Sarah
Publisher: name: Forschungzentrum Jülich
Year: 2023

description: INM Retreat, Jülich, Germany, 17 Oct 2023 - 18 Oct 2023
Saturation mutagenesis of a major histocompatibility complex protein domain: identification of a single conserved amino acid important for allorecognition.
Creators: name: Murray, R.
name: Frelinger, J. A.
name: Hutchison, C. A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1988

description: The interactive association between T lymphocytes and their target cells is an important system of cell-cell interactions. Major histocompatibility complex class I molecules are the cell surface structures recognized by cytolytic T lymphocytes. To define the molecular structures recognized by cytotoxic T lymphocytes, we have saturated the 270-base-pair alpha 1 exon of the H-2Dp gene with point mutations, rapidly producing a "library" of 2.5 x 10(3) independent mutants. The library contains enough recombinant clones (each clone encoding approximately one amino acid replacement mutation) to predict a mutation at each nucleotide position of the alpha 1 exon. The functional analysis of the first five transfected gene products tested has shown that mutation of a conserved tyrosine at position 27 to asparagine destroys recognition of the H-2Dp gene product by polyclonal alloreactive cytotoxic T lymphocytes. Recognition of the same mutant molecule by three monoclonal antibodies and H-2-restricted lymphocytic choriomenengitis virus-specific cytotoxic T lymphocytes is unaffected.
Thymine dimers bend DNA.
Creators: name: Sancar, A.
name: Griffith, J.
name: Husain, I.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1988

description: A 32-base-pair DNA fragment containing a thymine photodimer was constructed and ligated head-to-tail to obtain multimers of this sequence in which thymine dimers were in phase with the helix screw axis (approximately equal to 3 turns apart). The ligation products were analyzed by one- and two-dimensional gel electrophoresis and quantitative electron microscopy. These analyses show that the thymine photodimer introduces a bend of approximately equal to 30 degrees in DNA, which causes anomalously slow migration of DNA fragments in polyacrylamide gels and facilitates the formation of small covalent circles. Repair of thymine dimers by DNA photolyase abolishes the anomalous migration.
Canine hemophilia B resulting from a point mutation with unusual consequences.
Creators: name: High, K. A.
name: Reisner, H. M.
name: Brayer, G. D.
name: Brinkhous, K. M.
name: Evans, J. P.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1989

description: We have used the polymerase chain reaction to amplify the entire coding region of canine factor IX from a hemophilia B animal. When the sequence was compared to that which codes for normal canine factor IX, a single missense mutation was identified. This mutation (G----A at nucleotide 1477) results in the substitution of glutamic acid for glycine-379 in the catalytic domain of the molecule. The mutation creates a new restriction site that allowed confirmation of the abnormal sequence in both hemophilic and carrier animals. Amino acid 379 in canine factor IX corresponds to position 381 in human factor IX, a location at which no human mutations have been described. Moreover, it occurs at one of the few amino acids that have been rigorously conserved among the trypsin-like serine proteases throughout evolution. The mutation responsible for canine hemophilia B results in a complete lack of circulating factor IX in the affected animals. As it is unusual for a missense mutation to result in a complete absence of protein product, structural modeling of the mutant and normal proteins was pursued. These studies suggest that the observed mutation would have major adverse effects on the tertiary structure of the aberrant factor IX molecule. The elucidation of this mutation sheds light on structure-function relationships in factor IX and should facilitate future experiments directed toward gene therapy of this disease.
Deletions of bases in one strand of duplex DNA, in contrast to single-base mismatches, produce highly kinked molecules: possible relevance to the folding of single-stranded nucleic acids.
Creators: name: Hsieh, C. H.
name: Griffith, J. D.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1989

description: A 32-base-pair (bp) DNA duplex with deletions in one strand, and thus extra bases in the opposing strand, was ligated head-to-tail to produce linear and circular multimers. The electrophoretic mobility of the linear multimers was analyzed in polyacrylamide gels and the size of the circular DNA was determined by electron microscopy. A 1-base deletion produced a marked retardation in the mobility of the linear multimers coincident with the formation of a population of multimeric circles of a smaller average size than the deletionless 32-mer; 2-, 3-, or 4-base deletions at the same site produced proportionately greater effects. Two 1-base deletions separated by 10 bp on the same strand produced a greater reduction in mobility than a 1-base deletion, whereas two 1-base deletions spaced by 5 bp on the same strand yielded a molecule that behaved more like the deletionless DNA. We conclude that deletions of 1-4 bases at a single site on duplex DNA produce molecules that behave as if they contain sharp bends or kinks. In contrast, single mismatches in the 32-bp duplex produced no abnormality in behavior relative to normally base-paired DNA in the gel mobility and electron microscopic assays. The possible role of such structures in organizing the three-dimensional folding of single-stranded nucleic acids is considered.
On genus one curves of degree 5 with square-free discriminant
Creators: name: Fisher, Tom
name: Sadek, Mohammad
Publisher: name: arXiv
Year: 2014
Subjects: subject: Number Theory (math.NT)
subject: FOS: Mathematics
subject: 11G07, 11G05, 14H25

description: We study genus one curves of degree 5 defined by Pfaffians. We give new formulae for the invariants, and prove the equivalence of two different definitions of minimality. As an application we show that transformations between models with square-free discriminant are necessarily integral. This result is used by Bhargava and Shankar in their work on the average ranks of elliptic curves.
description: 25 pages
"Verehrungswürdiger als die Cherubim ..."
Creators: name: Kasselurē-Chatzēbasileiadē, Elenē B.
Publisher: name: Concilium
Year: 1970

description: Concilium, Bd. 44 Nr. 4 (2008): Concilium: Internationale Zeitschrift für Theologie, Band 44, Heft 4
Differences in the rates of gene amplification in nontumorigenic and tumorigenic cell lines as measured by Luria-Delbruck fluctuation analysis.
Creators: name: Tlsty, T. D.
name: Lum, K.
name: Margolin, B. H.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1989

description: It has been hypothesized that genomic fluidity is an important component of tumorigenesis. Previous studies described the relationship between tumorigenicity and one marker for genomic fluidity, gene amplification. In this report, these studies are extended with the rat liver epithelial cell lines to show that: (i) the amplification in these cells arises in a spontaneous fashion in the population (i.e., the variants detected are not preexisting in the population), and (ii) the rate of spontaneous amplification (mutation), as measured by Luria-Delbrück fluctuation analysis, is significantly lower in the nontumorigenic cells than in the tumorigenic cells. The rate was estimated by using the Po method and the method of means. The rate of spontaneous amplification of the gene encoding the multifunctional protein CAD (containing the enzymatic activities carbamoyl-phosphate synthase, aspartate transcarbamylase, and dihydroorotase) in the highly tumorigenic cells was significantly greater than that for the nontumorigenic cells, reaching almost 1 x 10(-4) events per cell per generation. The rate of this mutagenic event is high compared to the rate of point mutations usually reported in mammalian cells, and its potential contribution to the tumorigenic process will be discussed.
DNA and spermidine provide a switch mechanism to regulate the activity of restriction enzyme Nae I.
Creators: name: Conrad, M.
name: Topal, M. D.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1989

description: Sequence-specific DNA-protein interactions are basic to DNA function. To better understand these interactions, we studied the effect of position on cleavage of DNA by the type II restriction enzyme (EC 3.1.21.4) Nae I. We discovered two classes of Nae I restriction sites: sites susceptible and sites resistant to cleavage. Kinetic analysis showed that Nae I was activated by DNA containing cleavable Nae I sites to rapidly cleave resistant Nae I sites by a noncompetitive mechanism with a Km for substrate DNA of about 2 nM and a KA for activating DNA of about 6 nM; activation increased catalysis but not substrate binding. Deletion mutagenesis in vitro showed that sequences flanking the Nae I recognition site were responsible for the differences between activating and nonactivating Nae I sites. The polyamine spermidine had a dramatic effect on the interaction of Nae I with DNA; in the presence of 1 mM spermidine, resistant sites were cleaved rapidly and cleavable DNA inhibited cleavage. The direct regulation of enzymatic activity by DNA sequences in trans, and the modulation of this regulation by a polyamine that is sensitive to the cell cycle, provides a regulatory switch mechanism. The implications of this switch for biological control functions are discussed.
title: Semaine de botanique alpine dans le Lötschental du 3 au 10 juillet 2021
title: Semaine de botanique alpine dans le Lötschental du 3 au 10 juillet 2021
titleType: AlternativeTitle
Creators: name: Blanchon, Catherine
name: Éberlé, Sylvine
name: Thiébault, Philippe
Publisher: name: Unknown
Year: 2022

description: La traditionnelle semaine de botanique alpine a conduit vingt-sept membres du Cercle Vaudois de Botanique dans le Valais et plus précisément dans le Lötschental. Nous résidons à l’hôtel Edelweiss de Blatten, tenu par M. et Mme Kalbermatten, hôtel moderne avec une bonne table et des hôtes attentifs. Nous travaillons chaque jour au retour des excursions dans une salle mise à notre disposition. Nous avons fait deux repérages, l’un en février pour choisir l’hôtel et un autre en mai pour repérer les lieux d’herborisations et profiter de la riche flore de printemps. Lors de ces sept journées d’herborisation, le CVB a contribué à l’inventaire de la flore du Valais, réalisé sous l’égide de l’association FloraVS. Toutes les observations saisies à partir de l’application FlorApp ont été transmises à InfoFlora. La nomenclature suit la Checklist 2017 (Juillerat et al., 2017) d’InfoFlora. Les codes de milieux suivent le Guide des milieux naturels de Suisse (Delarze et al., 2015).
Effect of recombinant factor VIIa on the hemostatic defect in dogs with hemophilia A, hemophilia B, and von Willebrand disease.
Creators: name: Hedner, U.
name: Brinkhous, K. M.
name: Diness, V.
name: Read, M. S.
name: Garris, J. B.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1989

description: Recombinant factor VIIa (rF.VIIa) is a two-chain procoagulant enzyme (M
Elevated dynorphin in the hippocampal formation of aged rats: relation to cognitive impairment on a spatial learning task.
Creators: name: Owyang, V. V.
name: Jiang, H. K.
name: Gallagher, M.
name: Hong, J. S.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1989

description: Radioimmunoassay revealed increased dynorphin A(1-8)-like immunoreactivity (dynA(1-8)LI) in the aged rat brain. Among a number of brain regions examined, an age-related dynA(1-8)LI elevation was found only in the hippocampal formation and frontal cortex. Moreover, the increase in dynA(1-8)LI in the aged hippocampus was associated with a decline in spatial learning ability: dynA(1-8)LI distinguished aged rats that were behaviorally impaired from aged cohorts that learned the spatial task as rapidly as younger animals. Northern blot hybridization using a {sup 32}P-labeled complementary RNA probe encoding rat prodynorphin indicated that the abundance of prodynorphin mRNA was also significantly increased in the hippocampal formation of aged rats with identified spatial learning impairments.
VLT/FLAMES spectroscopy of red giant branch stars in the Fornax dwarf spheroidal galaxy
Creators: name: Lemasle, B.
name: de Boer, T.
name: Hill, V.
name: Tolstoy, E.
name: Irwin, M.
name: Jablonka, P.
name: Venn, K.
name: Battaglia, G.
name: Starkenburg, E.
name: Shetrone, M.
name: Letarte, B.
name: Francois, P.
name: Helmi, A.
name: Primas, F.
name: Kaufer, A.
name: Szeifert, T.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Astrophysics of Galaxies (astro-ph.GA)
subject: FOS: Physical sciences

description: Fornax is one of the most massive dwarf spheroidal galaxies in the Local Group. The Fornax field star population is dominated by intermediate age stars but star formation was going on over almost its entire history. It has been proposed that Fornax experienced a minor merger event. Despite recent progress, only the high metallicity end of Fornax field stars ([Fe/H]>-1.2 dex) has been sampled in larger number via high resolution spectroscopy. We want to better understand the full chemical evolution of this galaxy by better sampling the whole metallicity range, including more metal poor stars. We use the VLT-FLAMES multi-fibre spectrograph in high-resolution mode to determine the abundances of several alpha, iron-peak and neutron-capture elements in a sample of 47 individual Red Giant Branch stars in the Fornax dwarf spheroidal galaxy. We combine these abundances with accurate age estimates derived from the age probability distribution from the colour-magnitude diagram of Fornax. Similar to other dwarf spheroidal galaxies, the old, metal-poor stars of Fornax are typically alpha-rich while the young metal-rich stars are alpha-poor. In the classical scenario of the time delay between SNe II and SNe Ia, we confirm that SNe Ia started to contribute to the chemical enrichment at [Fe/H] between -2.0 and -1.8 dex. We find that the onset of SNe Ia took place between 12-10 Gyrs ago. The high values of [Ba/Fe], [La/Fe] reflect the influence of SNe Ia and AGB stars in the abundance pattern of the younger stellar population of Fornax. Our findings of low [alpha/Fe] and enhanced [Eu/Mg] are compatible with an initial mass function that lacks the most massive stars and with star formation that kept going on throughout the whole history of Fornax. We find that massive stars kept enriching the interstellar medium in alpha-elements, although they were not the main contributor to the iron enrichment.
description: Resubmitted to A&A (18/09/2014) after Referee's comments
GENERATION Z TEACHER PERCEPTIONS OF PRINCIPAL POWER AND THEIR SATISFACTION WITH SUPERVISION
Creators: name: Biddix, Christopher Andrew
Publisher: name: Purdue University Graduate School
Year: 2023
Subjects: classificationCode: 390307
subject: Teacher education and professional development of educators
classificationCode: 390403
subject: Educational administration, management and leadership

description: Gen Z public school teachers in Indiana were surveyed concerning their perceptions of power used by their principal and their satisfaction with their principal’s supervision. One hundred and forty-five teachers responded to survey items regarding whether they have worked under one or more principals, the community type (rural, urban, suburban) where they teach, the Rahim Leader Power Inventory (RLPI) to measure perceptions of principal’s use of six different available power bases (coercive, reward, legitimate, information, expert, referent) and the abridged Job Descriptive Index (aJDI) to measure satisfaction. Reward power was the most perceived power base and referent power the least. Two statistically significant findings were revealed. First, the more participants perceived their principal using expert (F(6,138) = 55.12, p < .001) and referent power (t(6) = -5.32, p < .001) bases, the less satisfied with supervision they were. Secondly, Gen Z teachers who had one principal perceived their principal to access more legitimate power than Gen Z teachers who have had more than principal (t(143) = 2.16, p =.032). To effectively influence this influential generation, educational leaders should consider autonomy, personalized growth, tactful integrity, and aligned expectations. Gen Z teachers value trust and transparency, and clashes with traditional leadership methods can lead to dissatisfaction. Meeting their expectations for integrity and authenticity is key to enhancing their satisfaction with supervision.
Escherichia coli DNA helicase II (uvrD gene product) catalyzes the unwinding of DNA.RNA hybrids in vitro.
Creators: name: Matson, S. W.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1989

description: DNA helicase II is a well-characterized Escherichia coli enzyme capable of unwinding duplex DNA and known to be involved in both methyl-directed mismatch repair and excision repair of pyrimidine dimers. Here it is shown that this enzyme also catalyzes the ATP-dependent unwinding of a DNA.RNA hybrid consisting of a radioactively labeled RNA molecule annealed on M13 single-stranded DNA. The DNA.RNA unwinding reaction required less protein to unwind more base pairs than the corresponding unwinding of duplex DNA. In addition, the rate of unwinding of the DNA.RNA hybrid was more than an order of magnitude faster than unwinding of a DNA partial duplex of similar length. The unwinding of the DNA.RNA hybrid is a property unique to helicase II since helicase I, Rep protein, and helicase IV failed to catalyze the reaction. In light of these results it seems likely that helicase II is involved in some previously unrecognized aspect of nucleic acid metabolism, in addition to its known roles in DNA repair reactions.
Excitation energy dependence of fission in the mercury region
Creators: name: McDonnell, J. D.
name: Nazarewicz, W.
name: Sheikh, J. A.
name: Staszczak, A.
name: Warda, M.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Nuclear Theory (nucl-th)
subject: FOS: Physical sciences

description: Background: Recent experiments on beta-delayed fission reported an asymmetric mass yield in the neutron-deficient nucleus 180Hg. Earlier experiments in the mass region A=190-200 close to the beta-stability line, using the (p,f) and (α,f) reactions, observed a more symmetric distribution of fission fragments. While the beta-delayed fission of 180Hg can be associated with relatively low excitation energy, this is not the case for light-ion reactions, which result in warm compound nuclei. Purpose: To elucidate the roles of proton and neutron numbers and excitation energy in determining symmetric and asymmetric fission yields, we compute and analyze the isentropic potential energy surfaces of 174,180,198Hg and 196,210Po. Methods: We use the finite-temperature superfluid nuclear density functional theory, for excitation energies up to E*=30MeV and zero angular momentum. For our theoretical framework, we consider the Skyrme energy density functional SkM* and a density-dependent pairing interaction. Results: For 174,180Hg, we predict fission pathways consistent with asymmetric fission at low excitation energies, with the symmetric fission pathway opening very gradually as excitation energy is increased. For 198Hg and 196Po, we expect the nearly-symmetric fission channel to dominate. 210Po shows a preference for a slightly asymmetric pathway at low energies, and a preference for a symmetric pathway at high energies. Conclusions: Our self-consistent theory suggests that excitation energy weakly affects the fission pattern of the nuclei considered. The transition from the asymmetric fission in the proton-rich nuclei to a more symmetric fission in the heavier isotopes is governed by the shell structure of pre-scission configurations.
description: 6 pages, 6 figures
Novel oxadiazole-thiadiazole derivatives: synthesis, biological evaluation, and in silico studies
Creators: name: Evren, Asaf Evrim
name: Nuha, Demokrat
name: Dawbaa, Sam
name: Karaduman, Abdullah Burak
name: Sağlik, Begüm Nurpelin
name: Yurttaş, Leyla
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Biophysics
subject: Biochemistry
subject: Cell Biology
subject: Pharmacology
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Information Systems not elsewhere classified
subject: Cancer
subject: Infectious Diseases
subject: FOS: Health sciences

description: In the search for new anticancer agents, we synthesized a new series of thiazole derivatives carried on thiadiazole-oxadiazole hybrid. Final compounds (5a–5i) were analyzed via 1H NMR, 13C NMR, and HRMS. The pharmacokinetic profile of the targeted compounds was predicted via in silico calculations. Their anticancer properties were determined using MTT method against MCF7 and A549 cell lines. Compounds 5a, 5b and 5c were found more active against MCF7 cells than A549 cells while they were not cytotoxic on L929 healthy cells. Generally, it can be summarized that acetamide moiety has a pivotal role in anticancer activity. For further studies, their aromatase inhibitory activity was evaluated. After determination all these features, the binding modes of the active compounds and the stability and relation of the ligand-enzyme complex were investigated using molecular docking and molecular dynamics simulation studies, respectively. In vitro and in silico studies suggest two important structure-activity relationship (SAR) points that at least one azole ring is essential, and if there is approximately 8.0 ± 0.5 Å distance between the H-bond rich zone of ligand and the heteroaryl ring system of ligand has a major impact on aromatase inhibitory activity. Compounds with small group substitution on thiazole are found potentially may be used for the treatment of anti-breast cancer orally. Communicated by Ramaswamy H. Sarma
Haplotype-specific differences in signaling by transfected class II molecules to a Ly-1+ B-cell clone.
Creators: name: Frelinger, J. A.
name: Bishop, G. A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1989

description: CH12.LX B cells are responsive to antigen-dependent differentiative signals transmitted through their surface Ek molecules. Although CH12.LX cells express surface Ak molecules with normal protein sequence, the Ak molecules do not deliver differentiative signal to these B cells. To determine whether introduction of a new A molecule into CH12.LX cells would correct this deficiency, CH12.LX cells were transfected with the genes encoding new Aalpha and/or Abeta molecules. It was found that transfected cells responded to antigen-specific signals delivered via the Ealphak Ebetak, Aalphab Abetab, or Aalphak Abetab molecule. However, the B cells did not respond to signals generated by the molecule Aalpha kAbetak, Aalphab Abetak, Aalphak Abetad, or Aalpha kAbetau. Comparison of these sequences suggests that two Abeta residues, His-47 and Trp-197, are important to the transmission of differentiative signals to B cells.
Identification of novel carbonic anhydrase II receptor-targeting drugs for treating myocardial infarction through the mechanism of Xue-Fu-Zhu-Yu decoction
Creators: name: Yan, Gaofei
name: Chen, Jing
name: Luo, Shufang
name: Zhang, Kaiyuan
name: Chen, Qi
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Biophysics
subject: Biochemistry
subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Pharmacology
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Information Systems not elsewhere classified
subject: Cancer

description: Myocardial infarction (MI) is a significant threat to human health and life. Xue-Fu-Zhu-Yu Decoction (XFZYD), a renowned traditional Chinese medicine prescription for treating myocardial infarction, is known to play a significant role in the management of MI. However, its mechanism of action remains unclear. Through network pharmacology analysis of compound-target interactions, we have identified Carbonic Anhydrase II (CA2) as a critical target for XFZYD in the treatment of MI. Subsequently, we will embark on a target-based drug design approach with a focus on CA2 as the key target: Pharmacophore modeling: Two pharmacophore models were developed and validated to screen for small molecules with CA2 inhibitory features. Virtual screening: Based on two pharmacophore models, small molecules with the property of binding to the CA2 target were screened from a virtual screening library. Molecular docking: Molecular docking was employed to identify small molecules with stable binding affinity to CA2. ADMET prediction: ADMET models were utilized to screen for small molecules with favorable pharmacological properties. Molecular dynamics: Molecular dynamics simulations were further conducted to analyze the binding modes of the selected small molecules with CA2, ultimately resulting in the identification of Ligand 3 and Ligand 5 as small molecule inhibitors targeting CA2. Finally, the mechanisms underlying the anti-MI effects were discussed. The primary objective of this article is to uncover the mechanism by which XFZYD acts on MI and utilize it for drug development. These findings provide novel avenues for the development of anti-MI drugs. Communicated by Ramaswamy H. Sarma
Puzzling Outer-Density Profile of the Dark Matter Halo in the Andromeda Galaxy
Creators: name: Kirihara, Takanobu
name: Miki, Yohei
name: Mori, Masao
Publisher: name: arXiv
Year: 2014
Subjects: subject: Astrophysics of Galaxies (astro-ph.GA)
subject: FOS: Physical sciences

description: The cold dark matter (CDM) cosmology, which is the standard theory of the structure formation in the universe, predicts that the outer density profile of dark matter halos decreases with the cube of distance from the center. However, so far not much effort has examined this hypothesis. In the halo of the Andromeda galaxy (M31), large-scale stellar structures detected by the recent observations provide a potentially suitable window to investigate the mass--density distribution of the dark matter halo. We explore the density structure of the dark matter halo in M31 using an N-body simulation of the interaction between an accreting satellite galaxy and M31. To reproduce the Andromeda Giant Southern Stream and the stellar shells at the east and west sides of M31, we find the sufficient condition for the power-law index $α$ of the outer density distribution of the dark matter halo. The best-fit parameter is $α=-3.7$, which is steeper than the CDM prediction.
description: 5 pages, 4 figures, accepted for publication in PASJ Letter
High-order fluorescence fluctuation analysis of model protein clusters.
Creators: name: Palmer, A. G.
name: Thompson, N. L.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1989

description: La précision avec laquelle une auto-corrélation de la fluctuation de la fluorescence détectée et la quantification de l'oligomérisation de la protéine peut être évaluée rigoureusement en utilisant des solutions contenant deux protéines fluorescentes.