title: ÐÐ¸Ð¾ÑÐ¸Ð»Ð¸Ð·Ð°ÑÐ¸Ñ Ð¸ Ð´Ð¾Ð»Ð³Ð¾ÑÑÐ¾ÑÐ½Ð¾Ðµ Ñ ÑÐ°Ð½ÐµÐ½Ð¸Ðµ ÑÐµÐ°Ð³ÐµÐ½ÑÐ¾Ð² Ð² Ð¼Ð¸ÐºÑÐ¾ÑÐ»ÑÐ¸Ð´Ð½ÑÑ ÑÑÑÑÐ¾Ð¹ÑÑÐ²Ð°Ñ Ð´Ð»Ñ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ Ð°Ð½Ð°Ð»Ð¸Ð·Ð¾Ð² Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ ÐÐ¦Ð Ð² ÑÐµÐ°Ð»ÑÐ½Ð¾Ð¼ Ð²ÑÐµÐ¼ÐµÐ½Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Freeze drying and long-term storage of the reagents for real-time PCR analysis in microfluidic devices
Creators: name: ÐÑÐ¸Ð³Ð¾ÑÑÐµÐ², ÐÐ¸ÑÐ°Ð»Ð¸Ð¹
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Ð¼Ð¸ÐºÑÐ¾ÑÐ»ÑÐ¸Ð´Ð¸ÐºÐ°
Ð¼Ð¸ÐºÑÐ¾ÑÐ¸Ð¿Ð¾Ð²ÑÐµ ÑÐµÑ Ð½Ð¾Ð»Ð¾Ð³Ð¸Ð¸
Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð½Ð°Ñ ÑÐµÐ¿Ð½Ð°Ñ ÑÐµÐ°ÐºÑÐ¸Ñ Ð² ÑÐµÐ°Ð»ÑÐ½Ð¾Ð¼ Ð²ÑÐµÐ¼ÐµÐ½Ð¸
Ð»Ð¸Ð¾ÑÐ¸Ð»Ð¸Ð·Ð°ÑÐ¸Ñ
ÑÐºÑÐ¿ÑÐµÑÑ-Ð°Ð½Ð°Ð»Ð¸Ð·
microfluidics
microchip technology
real-time (quantitative) polymerase chain reaction
lyophilization
rapid analysis

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description: The subject of the graduate qualification work is âFreeze drying and long-term storage of the reagents for real-time PCR analysis in microfluidic devicesâ. The given work is devoted to determine the six-month-long storage possibility of freeze-dried reagents mix used in real-time (quantitative) polymerase chain reaction (qPCR) inside of microfluidic device (chip) chambers. Parameters observed during the research were the polymerase activity indicators: threshold cycle â because the enzyme is the least stable component. To reach the goal, the following steps are requiredDevelop a protocol and lyophilize wet reagents mixture inside microfluidic chip chambersPerform a range of qPCR analyses and determine threshold cycle.Perform statistical analysis: compere samples of polymerase activity indicators belonging to control and experimental groups (differ in the storage time). Freeze-drying was performed in the Laboratory of Nanobiotechnologies (Alferov University), qPCR was executed in the Laboratory of information and measurement bio- and chemosensor microsystems (IAI RAS), where the microchips were also made. The premixed reagents kit Â«GM-101Â» (SYNTOL, Russia) was used in the study, an included positive control was used as the DNA sample.As a result, it was shown that it is possible to store the reagents for six months at a temperature of +4Â Â°C without decreasing their efficiency, that would be used for developing of qPCR preforming automatized microfluidic devices.