title: ÐÐ¿ÑÐ¸Ð¼Ð¸Ð·Ð°ÑÐ¸Ñ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ñ Ð°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸Ðº ÑÑÐ°Ð¼Ð¼Ð° Ð¶Ð¸Ð²Ð¾Ð¹ Ð³ÑÐ¸Ð¿Ð¿Ð¾Ð·Ð½Ð¾Ð¹ Ð²Ð°ÐºÑÐ¸Ð½Ñ, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð¾Ð±ÑÐ°ÑÐ½Ð¾Ð¹ Ð³ÐµÐ½ÐµÑÐ¸ÐºÐ¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Optimization of the biological characteristics of a strain of live influenza vaccine obtained by the method of reverse genetics
Creators: name: ÐÑÐ°Ð½Ð°ÑÑÐµÐ²Ð°, ÐÐ½Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð²Ð¸ÑÑÑ Ð³ÑÐ¸Ð¿Ð¿Ð°
ÑÐµÐ°ÑÑÐ¾ÑÑÐ°Ð½ÑÐ½ÑÐ¹ ÑÑÐ°Ð¼Ð¼
Ð³ÑÐ¸Ð¿Ð¿Ð¾Ð·Ð½ÑÐµ Ð²Ð°ÐºÑÐ¸Ð½Ñ
Ð¿Ð°Ð½Ð´ÐµÐ¼Ð¸ÑÐµÑÐºÐ¸Ð¹ Ð²Ð¸ÑÑÑ
Ð¾Ð±ÑÐ°ÑÐ½Ð°Ñ Ð³ÐµÐ½ÐµÑÐ¸ÐºÐ°
influenza virus
reassortant strain
influenza vaccines
pandemic virus
reverse genetics

description: Ð¦ÐµÐ»ÑÑ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÐµÑÑÑ Ð¾Ð¿ÑÐ¸Ð¼Ð¸Ð·Ð°ÑÐ¸Ñ ÑÐ²Ð¾Ð¹ÑÑÐ² ÑÑÐ°Ð¼Ð¼Ð° Ð¶Ð¸Ð²Ð¾Ð¹ Ð³ÑÐ¸Ð¿Ð¿Ð¾Ð·Ð½Ð¾Ð¹ Ð²Ð°ÐºÑÐ¸Ð½Ñ, ÑÐ¾Ð·Ð´Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð¾Ð±ÑÐ°ÑÐ½Ð¾Ð¹ Ð³ÐµÐ½ÐµÑÐ¸ÐºÐ¸, Ð² ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²Ð¸Ð¸ Ñ ÐºÑÐ¸ÑÐµÑÐ¸ÑÐ¼Ð¸ Ð±ÐµÐ·Ð¾Ð¿Ð°ÑÐ½Ð¾ÑÑÐ¸ Ð¸ Ð²ÑÑÐ¾ÐºÐ¾Ð¹ ÑÐµÐ¿ÑÐ¾Ð´ÑÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð² ÑÐ°Ð·Ð²Ð¸Ð²Ð°ÑÑÐ¸ÑÑÑ ÐºÑÑÐ¸Ð½ÑÑ ÑÐ¼Ð±ÑÐ¸Ð¾Ð½Ð°Ñ. Ð ÐµÐ°ÑÑÐ¾ÑÑÐ°Ð½ÑÐ½ÑÐ¹ ÑÑÐ°Ð¼Ð¼ Ð¿Ð¾Ð»ÑÑÐµÐ½ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð¾Ð±ÑÐ°ÑÐ½Ð¾Ð¹ Ð³ÐµÐ½ÐµÑÐ¸ÐºÐ¸ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ ÑÐ¿Ð¸Ð´ÐµÐ¼Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÑÐ°Ð¼Ð¼Ð° Ð/Canberra/1/2022(H3N2),Â  Ð¸ ÑÐ½Ð¸Ð²ÐµÑÑÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð²ÑÑÐ¾ÐºÐ¾ÑÐµÐ¿ÑÐ¾Ð´ÑÐºÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ Ð´Ð¾Ð½Ð¾ÑÐ° A/Leningrad/134/17/57 (H2N2)C. Ð ÐµÐ¿ÑÐ¾Ð´ÑÐºÑÐ¸Ð²Ð½Ð°Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÑÑÐ°Ð¼Ð¼Ð° Ð² ÑÐ¸ÑÑÐµÐ¼Ðµ ÑÐ°Ð·Ð²Ð¸Ð²Ð°ÑÑÐ¸ÑÑÑ ÐºÑÑÐ¸Ð½ÑÑ ÑÐ¼Ð±ÑÐ¸Ð¾Ð½Ð¾Ð² Ð¸ ÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾Ð¹ ÑÐ¾ÑÐ¼ÑÐ»Ð¾Ð¹ Ð³ÐµÐ½Ð¾Ð¼Ð° 6:2 Ñ Ð³ÐµÐ¼Ð°Ð³Ð³Ð»ÑÑÐ¸Ð½Ð¸Ð½Ð¾Ð¼ Ð¾Ñ ÑÑÐ°Ð¼Ð¼Ð° Ð´Ð¸ÐºÐ¾Ð³Ð¾ ÑÐ¸Ð¿Ð° Ð±ÑÐ»Ð° Ð¿Ð¾Ð´Ð½ÑÑÐ° Ð´Ð¾ Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼Ð¾Ð³Ð¾ ÑÑÐ¾Ð²Ð½Ñ Ð´Ð»Ñ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´ÑÑÐ²Ð° Ð²Ð°ÐºÑÐ¸Ð½. Ð¤ÐµÐ½Ð¾ÑÐ¸Ð¿Ð¸ÑÐµÑÐºÐ¸Ðµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½Ð¾Ð³Ð¾ RG ÑÑÐ°Ð¼Ð¼Ð° ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²ÑÑÑ Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼ÑÐ¼ Ð´Ð»Ñ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ¸ Ð¶Ð¸Ð²Ð¾Ð¹ Ð³ÑÐ¸Ð¿Ð¿Ð¾Ð·Ð½Ð¾Ð¹ Ð²Ð°ÐºÑÐ¸Ð½Ñ. Ð¨ÑÐ°Ð¼Ð¼ Ð¾Ð±Ð»Ð°Ð´Ð°ÐµÑ Ð°ÑÑÐµÐ½ÑÐ¸ÑÑÑÑÐ¸Ð¼Ð¸ Ð¼ÑÑÐ°ÑÐ¸ÑÐ¼Ð¸, ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²ÑÑÑÐ¸Ð¼Ð¸ Ð´Ð¾Ð½Ð¾ÑÑ Ð°ÑÑÐµÐ½ÑÐ°ÑÐ¸Ð¸ Ð/Leningrad/134/17/57 (H2N2), Ð° ÑÐ°Ðº Ð¶Ðµ Ð¿ÑÐ¸Ð¾Ð±ÑÐµÐ» Ð¼ÑÑÐ°ÑÐ¸Ð¸ ÑÐ°ÑÐ°ÐºÑÐµÑÐ½ÑÐµ Ð´Ð»Ñ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ñ Ð² ÐºÐ»ÐµÑÐºÐ°Ñ Ð¿ÑÐ¸Ñ.
description: The purpose of this work is to optimize the properties of a strain of live influenza vaccine created by the method of reverse genetics, in accordance with the criteria of safety and high reproduction in developing chicken embryos. The reassortant strain was obtained by reverse genetics based on the epidemic strain A/Canberra/1/2022(H3N2), and the universal highly productive donor A/Leningrad/134/17/57 (H2N2)C. The reproductive activity of the strain in the system of developing chicken embryos and the stable 6:2 genome formula with hemagglutinin from the wild type strain was raised to the required level for the production of vaccines. The phenotypic properties of the obtained RG strain correspond to those necessary for the development of a live influenza vaccine. The strain has attenuating mutations corresponding to the attenuation donor A/Leningrad/134/17/57 (H2N2), as well as acquired mutations characteristic of development in bird cells.