Low Rates of Antimicrobial-Resistant Enterobacteriaceae in Wildlife in Taï National Park, Côte d’Ivoire, Surrounded by Villages with High Prevalence of Multiresistant ESBL-Producing Escherichia coli in People and Domestic Animals
Creators: name: Albrechtova, Katerina
name: Papousek, Ivo
name: Nys, Helene De
name: Pauly, Maude
name: Anoh, Etile
name: Mossoun, Arsene
name: Dolejska, Monika
name: Masarikova, Martina
name: Metzger, Sonya
name: Couacy-Hymann, Emmanuel
name: Akoua-Koffi, Chantal
name: Wittig, Roman M.
name: Klimes, Jiri
name: Cizek, Alois
name: Leendertz, Fabian
name: Literak, Ivan
Publisher: name: Robert Koch-Institut
Year: 2014
Subjects: subject: Bacterial
subject: Animals
subject: Humans
subject: Escherichia coli/genetics
subject: Prevalence
subject: Drug Resistance Multiple
subject: Escherichia coli/drug effects
subject: Escherichia coli Infections/microbiology
subject: beta-Lactamases/genetics
subject: Bacterial Proteins/metabolism
subject: Bacterial Proteins/genetics
subject: Escherichia coli Infections/epidemiology
subject: Anti-Bacterial Agents/pharmacology
subject: Dogs
subject: Microbial Sensitivity Tests
subject: beta-Lactamases/metabolism
subject: Plasmids/metabolism
subject: Cats
subject: Animals Domestic
subject: Animals Wild/microbiology
subject: Citrobacter freundii/genetics
subject: Citrobacter freundii/isolation &amp; purification
subject: Cote d'Ivoire
subject: Enterobacteriaceae/drug effects
subject: Enterobacteriaceae/genetics
subject: Enterobacteriaceae/isolation &amp; purification
subject: Enterobacteriaceae Infections/epidemiology
subject: Enterobacteriaceae Infections/microbiology
subject: Plasmids/genetics
subject: Quinolones/pharmacology
subject: 610 Medizin

description: Antimicrobial resistance genes can be found in all ecosystems, including those where antibiotic selective pressure has never been exerted. We investigated resistance genes in a collection of faecal samples of wildlife (non-human primates, mice), people and domestic animals (dogs, cats) in Côte d’Ivoire; in the chimpanzee research area of Taï National Park (TNP) and adjacent villages. Single bacteria isolates were collected from antibiotic-containing agar plates and subjected to molecular analysis to detect Enterobacteriaceae isolates with plasmid-mediated genes of extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR). While the prevalence of ESBL-producing E. coli in the villages was 27% in people (n = 77) and 32% in dogs (n = 38), no ESBL-producer was found in wildlife of TNP (n = 75). PMQR genes, mainly represented by qnrS1, were also present in human- and dog-originating isolates from the villages (36% and 42% in people and dogs, respectively), but no qnrS has been found in the park. In TNP, different variants of qnrB were detected in Citrobacter freundii isolates originating non-human primates and mice. In conclusion, ESBL and PMQR genes frequently found in humans and domestic animals in the villages were rather exceptional in wildlife living in the protected area. Although people enter the park, the strict biosecurity levels they are obliged to follow probably impede transmission of bacteria between them and wildlife.