title: ÐÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ Ð¼ÐµÑÐ¾Ð´Ð° ÑÑÐ°Ð³Ð¼ÐµÐ½ÑÐ½Ð¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð´Ð»Ñ Ð¸Ð´ÐµÐ½ÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Ð- Ð¸ Ð¢-ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð»Ð¸Ð¼ÑÐ¾Ð¼ ÑÐ¾Ð±Ð°Ðº
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: The application of the method of fragment analysis for the identification of B- and T-cell lymphomas of dogs
Creators: name: ÐÐ¾ÑÐ°ÑÐµÐ², ÐÐ¾Ð½ÑÑÐ°Ð½ÑÐ¸Ð½
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: ÐÐ¸Ð¾ÑÐµÑ Ð½Ð¾Ð»Ð¾Ð³Ð¸Ñ
Ð»Ð¸Ð¼ÑÐ¾Ð¼Ð° ÑÐ¾Ð±Ð°Ðº
Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÑÐµÑÐºÐ°Ñ ÑÐµÑÑ-ÑÐ¸ÑÑÐµÐ¼Ð°
Ð¾Ð»Ð¸Ð³Ð¾Ð½ÑÐºÐ»ÐµÐ¾ÑÐ¸Ð´Ð½ÑÐ¹ ÑÐ¸Ð½ÑÐµÐ·
ÑÑÐ°Ð³Ð¼ÐµÐ½ÑÐ½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð·
Ð²-ÐºÐ»ÐµÑÐ¾ÑÐ½Ð°Ñ Ð»Ð¸Ð¼ÑÐ¾Ð¼Ð° ÑÐ¾Ð±Ð°Ðº
Ñ-ÐºÐ»ÐµÑÐ¾ÑÐ½Ð°Ñ Ð»Ð¸Ð¼ÑÐ¾Ð¼Ð° ÑÐ¾Ð±Ð°Ðº
canine lymphoma
diagnostic test system
oligonucleotide synthesis
fragment analysis
canine b-cell lymphoma
canine t-cell lymphoma

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«ÐÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ Ð¼ÐµÑÐ¾Ð´Ð° ÑÑÐ°Ð³Ð¼ÐµÐ½ÑÐ½Ð¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð´Ð»Ñ Ð¸Ð´ÐµÐ½ÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Ð- Ð¸ Ð¢-ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð»Ð¸Ð¼ÑÐ¾Ð¼ ÑÐ¾Ð±Ð°ÐºÂ». Ð¦ÐµÐ»ÑÑ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÐµÑÑÑ ÑÐ¾Ð·Ð´Ð°Ð½Ð¸Ðµ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÑÐµÑÐºÐ¾Ð¹ ÑÐµÑÑ-ÑÐ¸ÑÑÐµÐ¼Ñ, Ð¾ÑÐ½Ð¾Ð²Ð°Ð½Ð½Ð¾Ð¹ Ð½Ð° Ð¼ÐµÑÐ¾Ð´Ðµ ÑÑÐ°Ð³Ð¼ÐµÐ½ÑÐ½Ð¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð°, Ð´Ð»Ñ Ð¸Ð´ÐµÐ½ÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ ÐºÐ»Ð¾Ð½Ð°Ð»ÑÐ½Ð¾ÑÑÐ¸ Ð- Ð¸ Ð¢-ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð»Ð¸Ð¼ÑÐ¾Ð¼ ÑÐ¾Ð±Ð°Ðº. ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: - ÐÐ¾Ð´Ð¾Ð±ÑÐ°ÑÑ Ð¼Ð°ÑÑÐ¸ÑÑ BCR Ð¸ TCR Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ National Center for Biotechnology Information (NCBI) Ð¸ ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²ÑÑÑÐ¸Ðµ Ð¸Ð¼ Ð¾Ð»Ð¸Ð³Ð¾Ð½ÑÐ»ÐºÐµÐ¾ÑÐ¸Ð´Ð½ÑÐµ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÐ¸. - ÐÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸ÑÑ ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÑ Ð¾ÑÐ¶Ð¸Ð³Ð°, ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÑ Ð¿Ð»Ð°Ð²Ð»ÐµÐ½Ð¸Ñ Ð¸ Ð¿ÑÐ¾Ð²ÐµÑÑÐ¸ Ð°Ð½Ð°Ð»Ð¸Ð· Ð´Ð¸Ð¼ÐµÑÐ¸Ð·Ð°ÑÐ¸Ð¸ Ð¿ÑÐ°Ð¹Ð¼ÐµÑÐ¾Ð² in silico. - ÐÑÐ¿Ð¾Ð»Ð½Ð¸ÑÑ Ð¾Ð»Ð¸Ð³Ð¾Ð½ÑÐºÐ»ÐµÐ¾ÑÐ¸Ð´Ð½ÑÐ¹ ÑÐ¸Ð½ÑÐµÐ· Ð¿Ð¾Ð´Ð¾Ð±ÑÐ°Ð½Ð½ÑÑ Ð¿ÑÐ°Ð¹Ð¼ÐµÑÐ¾Ð². - ÐÑÐ´ÐµÐ»Ð¸ÑÑ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ Ð¾Ð±ÑÐ°Ð·ÑÐ¾Ð² Ð¸Ð· Ð¿Ð°ÑÐ°ÑÐ¸Ð½Ð¾Ð²ÑÑ Ð±Ð»Ð¾ÐºÐ¾Ð² FFPE Ð¸ Ð¸Ð·Ð¼ÐµÑÐ¸ÑÑ Ð² Ð½Ð¸Ñ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ ÐÐÐ. - ÐÑÑÑÐµÑÑÐ²Ð¸ÑÑ ÑÐ±Ð¾ÑÐºÑ ÑÐµÑÑ-ÑÐ¸ÑÑÐµÐ¼Ñ Ð¸ Ð¿ÑÐ¾Ð²ÐµÑÐºÑ ÐµÐµ ÑÐ°Ð±Ð¾ÑÐ¾ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÐ¸. ÐÐ±ÑÐµÐºÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ â ÑÑÐ°ÑÑÐºÐ¸ BCR Ð¸ TCR, Ð¾ÑÐ²ÐµÑÑÑÐ²ÐµÐ½Ð½ÑÐµ Ð·Ð° Ð¿ÑÐ¾Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð- Ð¸ Ð¢-ÐºÐ»ÐµÑÐ¾Ðº Ð¿ÑÐ¸ Ð»Ð¸Ð¼ÑÐ¾Ð¼Ð°Ñ ÑÐ¾Ð±Ð°Ðº; Ð³ÐµÐ½Ð¾Ð¼ Canis lupus. Ð ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð¾ÑÐ½Ð¾Ð²Ð½ÑÑ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð²ÑÐ±ÑÐ°Ð½Ñ ÑÑÐ°Ð³Ð¼ÐµÐ½ÑÐ½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð·, Ð°Ð½Ð°Ð»Ð¸Ð· Ð¾Ð»Ð¸Ð³Ð¾Ð½ÑÐ»ÐºÐµÐ¾ÑÐ¸Ð´Ð¾Ð² in silico, Ð¾Ð»Ð¸Ð³Ð¾Ð½ÑÐºÐ»ÐµÐ¾ÑÐ¸Ð´Ð½ÑÐ¹ ÑÐ²ÐµÑÐ´Ð¾ÑÐ°Ð·Ð½ÑÐ¹ ÑÐ¸Ð½ÑÐµÐ·. Ð Ð°Ð±Ð¾ÑÐ° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ ÐÐÐ Â«ÐÐÐÐÂ». ÐÑÐ¾Ð±ÑÐ°Ð½Ð½ÑÐµ Ð¼Ð°ÑÑÐ¸ÑÑ ÑÐµÐ³Ð¸Ð¾Ð½Ð¾Ð² BCR Ð¸ TCR Ð¸ Ð¿Ð¾Ð´Ð¾Ð±ÑÐ°Ð½Ð½ÑÐµ Ð¸ Ð¿ÑÐ¾Ð°Ð½Ð°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐµ Ð¿ÑÐ°Ð¹Ð¼ÐµÑÑ Ð² ÑÐ¾Ð²Ð¾ÐºÑÐ¿Ð½Ð¾ÑÑÐ¸ Ñ ÑÐ¸Ð·Ð¸ÐºÐ¾-ÑÐ¸Ð¼Ð¸ÑÐµÑÐºÐ¸Ð¼Ð¸ Ð¿ÑÐµÐ¸Ð¼ÑÑÐµÑÑÐ²Ð°Ð¼Ð¸ ÑÑÐ°Ð³Ð¼ÐµÐ½ÑÐ½Ð¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð¿Ð¾Ð·Ð²Ð¾Ð»Ð¸Ð»Ð¸ Ð´Ð¾Ð±Ð¸ÑÑÑÑ Ð²ÑÑÐ¾ÐºÐ¾Ð¹ ÑÑÐ²ÑÑÐ²Ð¸ÑÐµÐ»ÑÐ½Ð¾ÑÑÐ¸ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÑÐµÑÐºÐ¾Ð¹ ÑÐµÑÑ-ÑÐ¸ÑÑÐµÐ¼Ñ. Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð½Ð°Ñ ÑÐµÑÑ-ÑÐ¸ÑÑÐµÐ¼Ð° Ð¼Ð¾Ð¶ÐµÑ Ð±ÑÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð° Ð´Ð»Ñ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐ¸ Ð-ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð¸ Ð¢-ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð»Ð¸Ð¼ÑÐ¾Ð¼ ÑÐ¾Ð±Ð°Ðº Ð¸Ð· Ð¿Ð¾ÑÐ°Ð¶ÐµÐ½Ð½ÑÑ ÑÐºÐ°Ð½ÐµÐ¹, Ð° ÑÐ°ÐºÐ¶Ðµ Ð´Ð»Ñ ÑÐ°Ð½Ð½ÐµÐ¹ Ð¸Ð´ÐµÐ½ÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Ð·ÑÐµÐ»ÑÑ Ð°ÐºÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ Ð¢- Ð¸ Ð-ÐºÐ»ÐµÑÐ¾Ðº Ð¸Ð· ÐºÑÐ¾Ð²Ð¸ Ð±Ð¾Ð»ÑÐ½ÑÑ Ð¶Ð¸Ð²Ð¾ÑÐ½ÑÑ. ÐÑÐ¸ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð¸Ð¸ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±Ð·Ð¾ÑÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ: eLIBRARY.RU, Elsevier ScienceDirect, Scopus, PubMed. Ð¡ÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÑÑ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»Ð¸ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ð½Ð¾Ð³Ð¾ Ð¾Ð±ÐµÑÐ¿ÐµÑÐµÐ½Ð¸Ñ Microsoft Excel.
description: The topic of the final qualifying work: Â«The application of the method of fragment analysis for the identification of B- and T-cell lymphomas of dogsÂ». The purpose of this work is to create a diagnostic test system based on the method of fragment analysis to identify the clonality of B- and T-cell lymphomas in dogs. Tasks that were solved during the research: - Select the BCR and TCR matrices using the National Center for Biotechnology Information (NCBI) and their corresponding oligonulkeotide sequences. - Determine the annealing temperatures, melting temperatures and analyze the dimerization of primers in silico. - Perform oligonucleotide synthesis of selected primers. - Isolate biological samples from FFPE paraffin blocks and measure DNA concentrations in them. - To assemble the test system and check its operability. The objects of research in this work are the BCR and TCR sites responsible for the production of B and T cells in canine lymphomas; the Canis lupus genome. Fragment analysis, in silico oligonucleotide analysis, and solid-phase oligonucleotide synthesis were selected as the main research methods. The work was carried out on the basis of NVLP LLC. The selected matrices of the BCR and TCR regions and the selected and analyzed primers, combined with the physico-chemical advantages of fragment analysis, made it possible to achieve high sensitivity of the diagnostic test system. The developed test system can be used to diagnose B-cell and T-cell lymphomas of dogs from affected tissues, as well as for early identification of mature activated T and B cells from the blood of sick animals. The following databases were used to perform the literature review: eLibrary.RU, Elsevier ScienceDirect, Scopus, PubMed. Statistical processing of experimental data was carried out using Microsoft Excel software.