title: ÐÐ·Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ ÑÐ²Ð¾Ð¹ÑÑÐ² Ð²Ð°ÐºÑÐ¸Ð½Ð½ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð² Ð¶Ð¸Ð²Ð¾Ð¹ Ð³ÑÐ¸Ð¿Ð¿Ð¾Ð·Ð½Ð¾Ð¹ Ð²Ð°ÐºÑÐ¸Ð½Ñ H3N2 Ð¿ÑÐ¸ Ð¸Ñ Ð°Ð´Ð°Ð¿ÑÐ°ÑÐ¸Ð¸ Ðº ÐºÑÐ»ÑÑÑÑÐµ ÐºÐ»ÐµÑÐ¾Ðº MDCK
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Changes in the biological properties of vaccine strains of LAIV H3N2 during their adaptation to MDCK cell culture
Creators: name: Ð¦Ð¸Ð¼Ð¼ÐµÑÐ¼Ð°Ð½, ÐÐºÐ°ÑÐµÑÐ¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÐÐ¸Ð¾ÑÐ¸Ð·Ð¸ÐºÐ°
ÐÐ»ÐµÑÐºÐ° (Ð±Ð¸Ð¾Ð».)
Ð¶Ð¸Ð²Ð°Ñ Ð³ÑÐ¸Ð¿Ð¿Ð¾Ð·Ð½Ð°Ñ Ð²Ð°ÐºÑÐ¸Ð½Ð°
Ð°Ð´Ð°Ð¿ÑÐ°ÑÐ¸Ð¾Ð½Ð½ÑÐµ Ð¼ÑÑÐ°ÑÐ¸Ð¸
Ð³ÐµÐ¼Ð°Ð³Ð³Ð»ÑÑÐ¸Ð½Ð¸Ð½
ÑÐµÑÐ¼Ð¾ÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾ÑÑÑ
live influenza vaccine
adaptive mutations
hemagglutinin
thermal stability

description: ÐÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ ÑÐ²Ð¾Ð¹ÑÑÐ² Ð²Ð°ÐºÑÐ¸Ð½Ð½Ð¾Ð³Ð¾ ÑÑÐ°Ð¼Ð¼Ð° Ð´Ð»Ñ Ð¶Ð¸Ð²Ð¾Ð¹ Ð°ÑÑÐµÐ½ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð¹ Ð³ÑÐ¸Ð¿Ð¿Ð¾Ð·Ð½Ð¾Ð¹ Ð²Ð°ÐºÑÐ¸Ð½Ñ A/17/Ð¢ÐµÑÐ°Ñ/12/30 (H3N2) Ð¿ÑÐ¸ ÐµÐ³Ð¾ Ð°Ð´Ð°Ð¿ÑÐ°ÑÐ¸Ð¸ Ðº ÐºÑÐ»ÑÑÑÑÐµ ÐºÐ»ÐµÑÐ¾Ðº MDCK. Ð Ð¿ÑÐ¾ÑÐµÑÑÐµ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ» Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½ Ð³Ð»ÑÐ±Ð¾ÐºÐ¸Ð¹ Ð°Ð½Ð°Ð»Ð¸Ð· ÑÐ¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð¹ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÑ Ð¿Ð¾ ÑÐ¾ÑÑÐ¾ÑÐ½Ð¸Ñ Ð¿ÑÐ¾Ð±Ð»ÐµÐ¼Ð°ÑÐ¸ÐºÐ¸ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´ÑÑÐ²Ð° ÐÐÐ, Ð¸ Ð±ÑÐ»Ð¾ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¾, ÑÑÐ¾ Ð°Ð»ÑÑÐµÑÐ½Ð°ÑÐ¸Ð²Ð½ÑÐ¼ ÑÐµÑÐµÐ½Ð¸ÐµÐ¼ ÑÑÑÐµÑÑÐ²ÑÑÑÐ¸Ñ Ð¿ÑÐ¾Ð±Ð»ÐµÐ¼ ÑÐ²Ð»ÑÐµÑÑÑ Ð¿ÐµÑÐµÑÐ¾Ð´ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´ÑÑÐ²Ð° ÐÐÐ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ ÐºÑÑÐ¸Ð½ÑÑ ÑÐ¼Ð±ÑÐ¸Ð¾Ð½Ð¾Ð² Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ ÑÑÐ±ÑÑÑÐ°ÑÐ° Ð½Ð°ÐºÐ¾Ð¿Ð»ÐµÐ½Ð¸Ñ Ð²Ð¸ÑÑÑÐ° Ð½Ð° ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐµ ÐºÑÐ»ÑÑÑÑÑ. ÐÐ´Ð½Ð°ÐºÐ¾, Ð²Ð¾Ð¿ÑÐ¾Ñ Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ð¸ Ð°Ð´Ð°Ð¿ÑÐ°ÑÐ¸Ð¾Ð½Ð½ÑÑ Ð¼ÑÑÐ°ÑÐ¸Ð¹ Ð½Ð° Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° Ð²Ð°ÐºÑÐ¸Ð½Ð½ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð² ÐÐÐ ÑÑÐµÐ±Ð¾Ð²Ð°Ð» Ð´Ð¾Ð¿Ð¾Ð»Ð½Ð¸ÑÐµÐ»ÑÐ½ÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹. ÐÐ»Ñ ÑÑÐ¾Ð³Ð¾ Ð±ÑÐ»Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ñ MDCK-Ð°Ð´Ð°Ð¿ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐµ ÑÑÐ°Ð¼Ð¼Ñ Ð¢ÐµÑ 17 Ð¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð°Ð¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾ÑÐ½ÑÑ Ð·Ð°Ð¼ÐµÐ½ Ð² Ð¼Ð¾Ð»ÐµÐºÑÐ»Ðµ Ð³ÐµÐ¼Ð°Ð³Ð³Ð»ÑÑÐ¸Ð½Ð¸Ð½Ð° Ð½Ð° ÑÐµÐ¿ÑÐ¾Ð´ÑÐºÑÐ¸Ñ, Ð¸Ð½ÑÐµÐºÑÐ¸Ð¾Ð½Ð½ÑÐµ ÑÐ¸ÑÑÑ Ð¸ ÑÐµÑÐ¼Ð¾ÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾ÑÑÑ Ð²Ð¸ÑÑÑÐ° A/17/Ð¢ÐµÑÐ°Ñ/12/30 (H3N2). Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð½ÑÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ¾Ð² Ð±ÑÐ»Ð¾ Ð²ÑÑÐ²Ð»ÐµÐ½Ð¾, ÑÑÐ¾Â  Ð°Ð¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾ÑÐ½ÑÐµ Ð·Ð°Ð¼ÐµÐ½Ñ: V176I, P215T, P221S, D265E Ð² HA1 Ð¸ W92G, N154K, D160H Ð² HA2 Ð¿Ð¾Ð²ÑÑÐ°ÑÑ ÑÐ¾ÑÑÐ¾Ð²ÑÐµ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ¸ Ð¸ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¾Ð½Ð½ÑÐµ ÑÐ¸ÑÑÑ Ð²Ð¸ÑÑÑÐ° A/17/Ð¢ÐµÑÐ°Ñ/12/30 (H3N2) Ð² ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ ÐºÑÐ»ÑÑÑÑÐµ MDCK, Ð² ÑÐ¾ Ð²ÑÐµÐ¼Ñ ÐºÐ°Ðº Ð°Ð¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾ÑÐ½Ð°Ñ Ð·Ð°Ð¼ÐµÐ½Ð° Y85E Ð¿Ð¾Ð½Ð¸Ð¶Ð°ÐµÑ ÑÐµÐ¿ÑÐ¾Ð´ÑÐºÑÐ¸Ñ Ð²Ð¸ÑÑÑÐ° Ð² ÐºÑÐ»ÑÑÑÑÐµ ÐºÐ»ÐµÑÐ¾Ðº MDCK. ÐÐ¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾ÑÐ½ÑÐµ Ð·Ð°Ð¼ÐµÐ½Ñ: P215T Ð² HA1 Ð¸ Y83H Ð² HA2 Ð¿Ð¾Ð²ÑÑÐ°ÑÑ ÑÐµÑÐ¼Ð¾ÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾ÑÑÑ Ð²Ð¸ÑÑÑÐ° A/17/Ð¢ÐµÑÐ°Ñ/12/30 (H3N2) Ð² ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ ÐºÑÐ»ÑÑÑÑÐµ MDCK, Ð² ÑÐ¾ Ð²ÑÐµÐ¼Ñ ÐºÐ°Ðº Ð°Ð¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾ÑÐ½Ð°Ñ Ð·Ð°Ð¼ÐµÐ½Ð° K124E Ð² HA1 ÑÐ½Ð¸Ð¶Ð°ÐµÑ ÑÐµÑÐ¼Ð¾ÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾ÑÑÑ Ð²Ð¸ÑÑÑÐ° Ð² ÐºÑÐ»ÑÑÑÑÐµ ÐºÐ»ÐµÑÐ¾Ðº MDCK.
description: The master's qualification work is devoited to the study of changes in the biological properties of vaccine strains of the LAIV virus A/17/Texas/12/30 H3N2 during its adaptation to MDCK cell culture. In the course of the work, a deep analysis of the current literature on the state of the problem of LAIV production was carried out, and it was determined that an alternative solution to existing problems is the transition of LAIV production using chiken embriose as a substrate for virus accumulation in cell cultures. However, the issue of the effect of adaptive mutations on the biological properties of LAIV vaccine strains requared additional research. For this, MDCK-adapted strains of the Tex17 were obtained and the effect of the amino acid substitutions inthe hemagglutinin molecule on reproduction, infectious titers and thermal stability of the A/17/Texas/12/30 H3N2 virus was studied. As a result of the experiments, it was found that amino acid substitutions: V176I, P215T, P221S, D265E in HA1 and W92G, N154K, D160H in HA2 increase the growth characteristics and infectious titers of the A/17/Texas/12/30 H3N2 virus in the cell culture, while the Y85E amino acid substitution reduces virus reproduction in MDCK cell culture. Amino acid substitutions: P215T in HA1 and Y83H in HA2 increase thermal stability of the A/17/Texas/12/30 H3N2 virus in MDCK cell culture, while amino acid substitution K124E in HA2 reduces thermal stability of the virus in MDCK cell culture.