Komarkovaea angustata Mai, Johansen et Pietrasiak 2018, sp. nov.
Creators: name: Mai, Truc
name: Johansen, Jeffrey R.
name: Pietrasiak, Nicole
name: Bohunická, Markéta
name: Martin, Michael P.
Publisher: name: Zenodo
Year: 2018
Subjects: Biodiversity
Taxonomy
Bacteria
Cyanobacteria
Cyanobacteriia
Synechococcales
Oculatellaceae
Komarkovaea
Komarkovaea angustata

description: Komarkovaea angustata Mai, Johansen et Pietrasiak sp. nov.   
 Diagnosis:— Phylogenetically closest to Tildeniella species, however, differing from that genus and all other genera in the Oculatellaceae by the basal unilateral bulge present in the Box-B helix (Fig. 7o).   
 Description:— Colony fasciculated, penetrating the agar, bright blue-green. Filaments lacking false branching, with variation in width between post-hormogonial and mature filaments, up to 3.9 μm wide (Fig. 22a–d). Sheath firm, thin, colorless, up to 0.7 μm wide. Trichomes constricted at cross-walls, rarely tapering (Figs. 22d), post-hormogonial trichomes 1.8–2.9 μm wide, mature trichomes 3.3–3.9 μm wide. Hormogonia (Figs. 22a–b) and necridia (Figs. 22b–c) present. Cells with parietal thylakoids, with up to 3 small granules per cell, sometimes with one small orange granule (Fig. 22e, at arrows), commonly cylindrical, especially in young trichomes, becoming isodiametric in actively growing trichomes; in more mature trichomes cells barrel-shaped, and pancake-like in meristematic zones (Fig. 22c–d), 2.2–5.7 μm long.   
D1-D1’ helix 63 ncleotides long, with basal 3’ unilateral bulge 7 nucleotides long (5’-CAUCCUA-3’), with one C/U mismatch at position 9/48, one unpaired Adenine residue at position 15 of the 5’ strand, and one large internal loop at position 21–23/33–37 immediately separated from the terminal loop by a 5’-GG:CC-3’ clamp. Terminal loop sequence 5’-ACAGU-3’ (Fig. 6p). Box B helix 41 nucleotides long, with one distinctive basal 3’ unilateral bulge at position 5/32–37, one internal loop at position 10–11/26–27; terminal loop with sequence 5’-AAUC-3’ (Fig. 7o). V2 helix 20 nucleotides long, with terminal loop of 4 nucleotides (Fig. 8k). V3 helix 87 nucleotides long, with multiple mismatches at position 13/77, 19/71, an unpaired adenine residue at position 9 on the 5’ strand, and three internal loops at position 5–6/83, 23–24/66–67 and 30–31/58–60 (Fig. 9m).  
 Etymology:— angustatus (L.): narrowed, referring to the trichomes which are occasionally narrowed towards the end, as well as the narrowed hormogonia.   
 Type locality:— Puerto Rico, El Yunque National Forest: Waterfall in the forest, 18°27.811’ N – 66°7.005’W collected in 2012 by Jay Hillery.   
 Holotype here designated:— Holotype BRY37783!, Herbarium for Nonvascular Cryptogams, Monte L. Bean Museum, Provo, Utah.   
 Reference strain: ―EY1- AM 2, Algal Culture Collection at John Carroll University, Cleveland, USA.   
 Taxonomic notes:— This genus is character-poor. The heterogeneity in trichome width and absence of false branches is characteristic, and could be compared to tapering species in Leptolyngbya: L. tenuis (Gomont 1892: 169) Anagnostidis & Komárek (1988: 393), L. laminosa (Gomont 1892: 167) Anagnostidis & Komárek (1988: 392) and L. fragilis (Gomont 1892: 163) Anagnostidis & Komárek (1988: 391). However, in every comparison the trichomes of K. angustata are wider. The species has low 16S rRNA gene sequence identity with the other genera in the Oculatellaceae (91.9-93.5%, see Table 5), strong evidence that it represents a separate genus-level lineage.
description: Published as part of Mai, Truc, Johansen, Jeffrey R., Pietrasiak, Nicole, Bohunická, Markéta & Martin, Michael P., 2018, Revision of the Synechococcales (Cyanobacteria) through recognition of four families including Oculatellaceae fam. nov. and Trichocoleaceae fam. nov. and six new genera containing 14 species, pp. 1-59 in Phytotaxa 365 (1) on pages 44-46, DOI: 10.11646/phytotaxa.365.1.1, http://zenodo.org/record/13704923