cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Substitution of Specific Amino Acids in Insulin-like Growth Factor (IGF) Binding Protein 5 Alters Heparin Binding and Its Change in Affinity for IGF-I in Response to Heparin
Creators: name: Arai, Takami
name: Clarke, Jane
name: Parker, Alex
name: Nam, Taek
name: Busby Jr., Walker
name: Clemmons, David R.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: Heparin binding to insulin-like growth factor (IGF)-binding protein 5 (IGFBP-5) leads to a 17-fold decrease in its affinity for IGF-I, and a region that contains several basic amino acids (Arg201-Arg218) may be involved in this affinity shift. In the present study, mutagenesis was used to analyze the effect of substitutions for basic amino acids in the Arg201-Arg218 region of IGFBP-5 on heparin-binding and the heparin-induced affinity shift. Nine mutant forms were prepared. Their association constants (Ka) for IGF-I were similar to native IGFBP-5. When 10 microg/ml of heparin was added, the Ka of native IGFBP-5 decreased 17-fold, and the Ka of the K134A/R136A mutant decreased 16-fold. In contrast, substitutions for specific basic amino acids in the Arg2O1-Arg218 region decrease the affinity shift to 1.1-3.2-fold. Lys 211 was especially important. When a mutant containing that single substitution was tested, heparin caused only a 2.5-fold reduction in IGF-I affinity. Affinity cross-linking studies showed that heparin was equipotent in inhibiting the formation of 125I-IGF-I-K134A/Rl36A mutant complexes compared to native IGFBP-5. In contrast, heparin had minimal effects on the formation of complexes between 125I-IGF-I and the other mutants. The heparin-binding activity of each mutant was determined. Four mutants, R201A/K202N, K202A/K206A/R207A, R201A/K202N/K206N/K208N, and K211N/R214A/K217A/R218A, had reduced heparin binding compared to native IGFBP-5. The other five mutants, including the K21IN mutant, showed no change in heparin binding. The four mutants with reduced heparin binding could be dissociated from heparin-Sepharose with much lower NaCl concentrations, indicating that they had reduced affinity. These findings suggest that Arg201 Lys202, LysS206, and Arg214 are important for heparin binding. In contrast, LyS211 is not important for the binding of IGFBP-5 to heparin, but substitution for it reduced the heparin-induced affinity shift.
Supporting figure S1 from Analysis of modularity and integration suggests evolution of dragonfly wing venation mainly in response to functional demands
Creators: name: Blanke, Alexander
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: Evolutionary Biology
subject: FOS: Biological sciences
subject: 110601 Biomechanics
subject: FOS: Health sciences

description: Phylomorphospace plots of the first three principal components of the uncorrected (A+C) and allometry and phylogeny corrected (B+D) landmark datasets for the forewings (A+B) and hindwings (C+D). The branching pattern is based on the phylogeny of Letsch et al. (2016).
Diffusion of Cu adatoms and dimers on Cu(111) and Ag(111) surfaces
Creators: name: Mińkowski, Marcin
name: Załuska--Kotur, Magdalena A.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Statistical Mechanics (cond-mat.stat-mech)
subject: FOS: Physical sciences

description: Diffusion of Cu adatoms and dimers on Cu(111) and Ag(111) surfaces is analyzed basing on ab-initio surface potentials. As compared to the simple single adatom jumps that alternate between two states, dimers undergo more complex diffusion process that combines translational and rotational moves. Diffusion coefficients for these processes on both surfaces are calculated and compared with diffusion of single particle. The main parameters of diffusion coefficient: energy barrier and prefactor become temperature dependent when dimer moves jumping between many positions of different values of energy. Small difference in the surface lattice constant for Cu and Ag crystal results in completely different energy landscape for dimer jumps and as an effect different diffusion process. At the same time single adatom diffusion does not change so much.
Supporting figure S2 from Analysis of modularity and integration suggests evolution of dragonfly wing venation mainly in response to functional demands
Creators: name: Blanke, Alexander
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: Evolutionary Biology
subject: FOS: Biological sciences
subject: 110601 Biomechanics
subject: FOS: Health sciences

description: Summary of the outlier analysis of wing shapes for fore- and hindwings before and after allometric and phylogenetic correction. The identified outlier species correspond to the triangles in Figure 2.
The Human Glucocorticoid Receptor Isoform: EXPRESSION, BIOCHEMICAL PROPERTIES, AND PUTATIVE FUNCTION
Creators: name: Oakley, Robert H.
name: Sar, Madhabananda
name: Cidlowski, John A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: Alternative splicing of the human glucocorticoid receptor (hGR) primary transcript produces two receptor isoforms, hGRalpha and hGRbeta, which differ at their carboxyl termini. The hGRalpha isoform conveys endocrine information to target tissues by altering patterns of gene expression in a hormone-dependent fashion. In contrast to hGRalpha, very little is known about the hGRbeta splice variant. Using hGRalpha- and hGRbeta-specific riboprobes on human multiple tissue Northern blots, we show that the hGRbeta message has a widespread tissue distribution. We also prove by reverse transcriptase-polymerase chain reaction that the alternative splicing event underlying the formation of the hGRbeta message occurs in these tissues. Because the hGRbeta protein differs from hGRalpha at the extreme COOH terminus, we investigated several of the biochemical properties of hGRbeta expressed in transfected cells. hGRbeta does not bind the glucocorticoid agonist dexamethasone nor the glucocorticoid antagonist RU38486 in vivo. Moreover, in contrast to hGRalpha, hGRbeta is located primarily in the nucleus of transfected cells independent of hormone administration. Finally, in the absence of hGRalpha, hGRbeta is transcriptionally inactive on a glucocorticoid-responsive enhancer. However, when both isoforms are expressed in the same cell, hGRbeta inhibits the hormone-induced, hGRalpha-mediated stimulation of gene expression. Thus, hGRbeta potentially functions as a dominant negative inhibitor of hGRalpha activity.
The Mitogen-activated Protein Kinase Phosphatases PAC1, MKP-1, and MKP-2 Have Unique Substrate Specificities and Reduced Activity in Vivo toward the ERK2 sevenmaker Mutation
Creators: name: Chu, Yanfang
name: Kelly, Kathleen
name: Solski, Patricia A.
name: Khosravi-Far, Roya
name: Der, Channing J.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: Mitogen-activated protein (MAP) kinases can be grouped into three structural families, ERK, JNK, and p38, which are thought to carry out unique functions within cells. We demonstrate that ERK, JNK, and p38 are activated by distinct combinations of stimuli in T cells that simulate full or partial activation through the T cell receptor. These kinases are regulated by reversible phosphorylation on Tyr and Thr, and the dual specific phosphatases PAC1 and MKP-1 previously have been implicated in the in vivo inactivation of ERK or of ERK and JNK, respectively. Here we characterize a new MAP kinase phosphatase, MKP-2, that is induced in human peripheral blood T cells with phorbol 12-myristate 13-acetate and is expressed in a variety of nonhematopoietic tissues as well. We show that the in vivo substrate specificities of individual phosphatases are unique. PAC1, MKP-2, and MKP-1 recognize ERK and p38, ERK and JNK, and ERK, p38, and JNK, respectively. Thus, individual MAP kinase phosphatases can differentially regulate the potential for cross-talk between the various MAP kinase pathways. A hyperactive allele of ERK2 (D319N), analogous to the Drosophila sevenmaker gain-of-function mutation, has significantly reduced sensitivity to all three MAP kinase phosphatases in vivo.
The American Soldier in World War II: Chemical Warfare Training
Creators: name: Research Branch, Information
Publisher: name: Roper Center for Public Opinion Research
Year: 2020

description: Rank in military; length of time overseas; recreation in Honolulu; gas mask; protection against gas attacks; gas chamber experience; chemical warfare training; use gas against Japan; Japan use gas against other countries; military discussions about war; orientation meetings; topics talked about in meetings; war information center; correspondence courses; skills taught during training; US/foreign countries relations after war.
Counting solutions of the Bethe equations of the quantum group invariant open XXZ chain at roots of unity
Creators: name: Gainutdinov, Azat M.
name: Hao, Wenrui
name: Nepomechie, Rafael I.
name: Sommese, Andrew J.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Mathematical Physics (math-ph)
subject: Statistical Mechanics (cond-mat.stat-mech)
subject: High Energy Physics - Theory (hep-th)
subject: Quantum Algebra (math.QA)
subject: Representation Theory (math.RT)
subject: FOS: Physical sciences
subject: FOS: Mathematics

description: We consider the sl(2)_q-invariant open spin-1/2 XXZ quantum spin chain of finite length N. For the case that q is a root of unity, we propose a formula for the number of admissible solutions of the Bethe ansatz equations in terms of dimensions of irreducible representations of the Temperley-Lieb algebra; and a formula for the degeneracies of the transfer matrix eigenvalues in terms of dimensions of tilting sl(2)_q-modules. These formulas include corrections that appear if two or more tilting modules are spectrum-degenerate. For the XX case (q=exp(i pi/2)), we give explicit formulas for the number of admissible solutions and degeneracies. We also consider the cases of generic q and the isotropic (q->1) limit. Numerical solutions of the Bethe equations up to N=8 are presented. Our results are consistent with the Bethe ansatz solution being complete.
description: 34 pages; v2: reference added; v3: two more references added and minor corrections
The Organic Crystallizing Agent 2-Methyl-2,4-pentanediol Reduces DNA Curvature by Means of Structural Changes in A-tracts
Creators: name: Harvey, Stephen C.
name: Harrington, Rodney E.
name: Griffith, Jack D.
name: Park, Kyusung
name: Dlakic, Mensur
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: Contemporary predictive models for sequence-dependent DNA structure provide a good estimation of overall DNA curvature in most cases. However, the two current models differ fundamentally in their view of the origin of DNA curvature. An earlier model that associates DNA bending primarily, although not exclusively, with stretches of adenines (A-tracts) is based on results of comparative gel retardation, cyclization kinetics, hydroxyl radical cutting, and other solution measurements. It represents an intersection of wedge and junction models. More recently, a non-A-tract bending model has been proposed, built on structural results from x-ray crystallography and molecular modeling. In this view, A-tracts are proposed to be straight and rigid, whereas mixed sequence DNA is bent. Because a key premise of the non-A-tract bending model is the crystallographic observation that A-tracts are straight, we have examined the effect in solution of 2-methyl-2,4-pentanediol (MPD), an organic solvent used in crystal preparation for crystallographic DNA structure determinations. Using cyclization analysis, DNase I cutting, chemical probing, and electron microscopy on DNA oligomers with and without A-tracts, we show that the presence of MPD in solution dramatically affects A-tracts and that the effect is specific to these sequence elements. Combined with the previous observation that MPD affects gel mobility of curved sequences with A-tracts, our findings support the bent A-tract model and call for caution in the interpretation of crystallographic results on DNA structure as these are presently obtained.
The Roles of Integrins and Extracellular Matrix Proteins in the Insulin-like Growth Factor I-stimulated Chemotaxis of Human Breast Cancer Cells
Creators: name: Jones, John I.
name: Doerr, Monica E.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: The effects of insulin-like growth factor I (IGF-I) on the migration of two human breast cancer cell lines, MCF-7 and MDA-231, were examined using a modified Boyden chamber. 10 ng/ml was the optimal IGF-I concentration for stimulation of migration. The majority of IGF-I-stimulated migration in both cell types was due to chemotaxis. MCF-7 cells failed to migrate on membranes coated with gelatin or fibronectin and migrated only in small numbers on laminin. In contrast, when vitronectin- or type IV collagen-coated membranes were used, the MCF-7 cells migrated in large numbers specifically in response to IGF-I but not to 10% fetal calf serum, epidermal growth factor, fibroblast growth factor, or platelet derived growth factor-BB. An IGF-I receptor-blocking antibody inhibited IGF-I-stimulated migration in both cell types. In addition, a blocking antibody to the alpha v beta 5 integrin (a vitronectin receptor) inhibited migration of MCF-7 cells in response to IGF-I through vitronectin but not through type IV collagen. Similarly, blocking antibodies specific for alpha 2 and beta 1 integrins significantly inhibited migration of both cell types through type IV collagen-coated membranes but not through vitronectin-coated membranes. We conclude that: 1) IGF-I stimulates migration of these two cell types through the IGF-I receptor; 2) interaction of vitronectin with the alpha v beta 5 integrin or collagen with the alpha 2 beta 1 integrin is necessary for the complete IGF-I response in MCF-7 cells, and 3) because migration represents an in vitro model for metastatic spread, integrins, extracellular matrix proteins, and IGF-I may play coordinated roles in the metastasis of breast cancer in vivo.
(2009) Frances Annie Stead
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Growth-regulated Hsp70 phosphorylation regulates stress responses and prion maintenance
Creators: name: Kao, Chung-Hsuan
name: 0000-0003-2285-9214
Publisher: name: The University of Texas at Austin
Year: 2020
Subjects: Hsp70
Phosphorylation
Protein homeostasis
Protein aggregation
Hsp104
Yeast
Prion

description: Maintenance of protein homeostasis in eukaryotes during normal growth and stress conditions requires the functions of Hsp70 chaperones and associated co-chaperones. Here we investigate an evolutionarily-conserved serine phosphorylation that occurs at the site of communication between the nucleotide-binding and substrate-binding domains of Hsp70. Ser151 phosphorylation in yeast Hsp70 (Ssa1) is promoted by cyclin-dependent kinase (Cdk1) during normal growth and dramatically affects heat shock responses, a function conserved with HSC70 S153 phosphorylation in human cells. Phospho-mimic forms of Ssa1 (S151D) also fail to relocalize in response to starvation conditions, do not associate in vivo with Hsp40 co-chaperones, Ydj1 and Sis1, and do not catalyze refolding of denatured proteins in vitro in cooperation with Ydj1 and Hsp104. S151 phosphorylation strongly promotes survival of heavy metal exposure and reduces Sup35-dependent [PSI⁺] prion activity, however, consistent with proposed roles for Ssa1 and Hsp104 in generating self-nucleating seeds of misfolded proteins. Taken together, these results suggest that Cdk1 downregulates Hsp70 function during periods of active growth, reducing propagation of aggregated proteins despite potential costs to overall chaperone efficiency.
Supplemental Material from Habitual stone-tool-aided extractive foraging in white-faced capuchins, Cebus capucinus.
Creators: name: Barrett, Brendan J.
name: Monteza-Moreno, Claudio M.
name: Dogandžić, Tamara
name: Zwyns, Nicolas
name: Ibáñez, Alicia
name: Crofoot, Margaret C.
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: Evolutionary Biology
subject: FOS: Biological sciences
subject: Ecology
subject: 60801 Animal Behaviour

description: Supplemental Figures and Tables
Transient Intermediates in the Thrombin Activation of Fibrinogen: EVIDENCE FOR ONLY THE desAA SPECIES
Creators: name: Gabriel, Don A.
name: Galanakis, Dennis
name: Li, Xiao
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: The structure of a fibrin gel depends on the nature of the fibrinogen activation products produced by thrombin and the physical condition under which assembly occurs. Two different structures of the intermediate fibrin protofibril have been proposed, the production of which requires different extents of fibrinopeptide A (FpA) cleavage from fibrinogen. The fibrin activation intermediates must be stable since time is required for the intermediates to diffuse to growing protofibrils. The classic Hall-Slayter model requires cleavage of both FpAs to form a desAA intermediate. The Hunziker model requires cleavage of only one FpA to form an AdesA intermediate. Electrophoretic quasi elastic light scattering has been used to show the time-dependent production of the relevant fibrinogen activation intermediates that includes desAA but not AdesA.
(2002) Hannah [Bare]
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
A Molecular Redox Switch on p21 ras: STRUCTURAL BASIS FOR THE NITRIC OXIDE-p21rasINTERACTION
Creators: name: Lander, Harry M.
name: Quilliam, Lawrence A.
name: Hajjar, David P.
name: Hempstead, Barbara L.
name: Mirza, Urooj A.
name: Campbell, Sharon
name: Chait, Brian T.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1997

description: We have identified the site of molecular interaction between nitric oxide (NO) and p21(ras) responsible for initiation of signal transduction. We found that p21(ras) was singly S-nitrosylated and localized this modification to a fragment of p21(ras) containing Cys118. A mutant form of p21(ras), in which Cys118 was changed to a serine residue and termed p21(ras)C118S, was not S-nitrosylated. NO-related species stimulated guanine nucleotide exchange on wild-type p21(ras), resulting in an active form, but not on p21(ras)C118S. Furthermore, in contrast to parental Jurkat T cells, NO-related species did not stimulate mitogen-activated protein kinase activity in cells transfected with p21(ras)C118S. These data indicate that Cys118 is a critical site of redox regulation of p21(ras), and S-nitrosylation of this residue triggers guanine nucleotide exchange and downstream signaling.
The Direct Detectability of Giant Exoplanets in the Optical
Creators: name: Greco, Johnny P.
name: Burrows, Adam
Publisher: name: arXiv
Year: 2015
Subjects: subject: Earth and Planetary Astrophysics (astro-ph.EP)
subject: FOS: Physical sciences

description: Motivated by the possibility that a coronagraph will be put on WFIRST/AFTA, we explore the direct detectability of extrasolar giant planets (EGPs) in the optical. We quantify a planet's detectability by the fraction of its orbit for which it is in an observable configuration ($f_\mathrm{obs}$). Using a suite of Monte Carlo experiments, we study the dependence of $f_\mathrm{obs}$ upon the inner working angle (IWA) and minimum achievable contrast ($C_\mathrm{min}$) of the direct-imaging observatory; the planet's phase function, geometric albedo, single-scattering albedo, radius, and distance from Earth; and the semi-major axis distribution of EGPs. We calculate phase functions for a given geometric or single-scattering albedo, assuming various scattering mechanisms. We find that the Lambertian phase function can predict significantly larger $f_\mathrm{obs}$'s with respect to the more realistic Rayleigh phase function. For observations made with WFIRST/AFTA's baseline capabilities ($C_\mathrm{min}\sim10^{-9}$, $\mathrm{IWA}\sim0.2''$), Jupiter-like planets orbiting stars within 10, 30, and 50 parsecs of Earth have volume-averaged observability fractions of ${\sim}$12%, 3%, and 0.5%, respectively. At 10 parsecs, such observations yield $f_\mathrm{obs}>1\%$ for low- to modest-eccentricity planets with semi-major axes in the range ${\sim}2 - 10$ AU. If $C_\mathrm{min}=10^{-10}$, this range extends to ${\sim}35$ AU. We find that, in all but the most optimistic configurations, the probability for detection in a blind search is low (${
description: 16 pages, 16 figures. Accepted for publication in ApJ
Roper Commercial Poll # 1946-025: World War II Veterans
Creators: name: Organization, The Roper
Publisher: name: Roper Center for Public Opinion Research
Year: 2020

description: Employment after the war (7); 1948 presidential election (2); best value for military construction is by government/privately owned companies (2); rate labor unions/business corporations (6); veteran's organizations (5); most important priority for Washington (2); GI loans (6); bank services (1); personal spending (1); need major/minor changes to make the US great (3); automobiles (16); 1944 presidential election (2).
(2003) Titus Bennett Stead
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
A Point Mutation in Escherichia coli DNA Helicase II Renders the Enzyme Nonfunctional in Two DNA Repair Pathways: EVIDENCE FOR INITIATION OF UNWINDING FROM A NICKIN VIVO
Creators: name: Matson, Steven W.
name: Brosh Jr., Robert M.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1997

description: Biosynthetic errors and DNA damage introduce mismatches and lesions in DNA that can lead to mutations. These abnormalities are susceptible to correction by a number of DNA repair mechanisms, each of which requires a distinct set of proteins. Escherichia coli DNA helicase II has been demonstrated to function in two DNA repair pathways, methyl-directed mismatch repair and UvrABC-mediated nucleotide excision repair. To define further the role of UvrD in DNA repair a site-specific mutant was characterized. The mutation, uvrDQ251E, resides within helicase motif III, a conserved segment of amino acid homology found in a superfamily of prokaryotic and eukaryotic DNA helicases. The UvrD-Q251E protein failed to complement the mutator and ultraviolet light-sensitive phenotypes of a uvrD deletion strain indicating that the mutant protein is inactive in both mismatch repair and excision repair. Biochemical characterization revealed a significant defect in the ability of the mutant enzyme to initiate unwinding at a nick. The elongation phase of the unwinding reaction was nearly normal. Together, the biochemical and genetic data provide evidence that UvrD-Q251E is dysfunctional because the mutant protein fails to initiate unwinding at the nick(s) used to initiate excision and subsequent repair synthesis. These results provide direct evidence to support the notion that helicase II initiates unwinding from a nick in vivo in mismatch repair and excision repair.
Supplemental Material from Habitual stone-tool-aided extractive foraging in white-faced capuchins, Cebus capucinus
Creators: name: Barrett, Brendan J.
name: Monteza-Moreno, Claudio M.
name: Dogandžić, Tamara
name: Zwyns, Nicolas
name: Ibáñez, Alicia
name: Crofoot, Margaret C.
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: Evolutionary Biology
subject: FOS: Biological sciences
subject: Ecology
subject: 60801 Animal Behaviour

description: Supplemental Figures and Tables
Multiscale nature of the dissipation range in gyrokinetic simulations of Alfvénic turbulence
Creators: name: Told, D.
name: Jenko, F.
name: TenBarge, J. M.
name: Howes, G. G.
name: Hammett, G. W.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Plasma Physics (physics.plasm-ph)
subject: Space Physics (physics.space-ph)
subject: FOS: Physical sciences

description: Nonlinear energy transfer and dissipation in Alfvén wave turbulence are analyzed in the first gyrokinetic simulation spanning all scales from the tail of the MHD range to the electron gyroradius scale. For typical solar wind parameters at 1 AU, about 30% of the nonlinear energy transfer close to the electron gyroradius scale is mediated by modes in the tail of the MHD cascade. Collisional dissipation occurs across the entire kinetic range $k_\perpρ_i\gtrsim 1$. Both mechanisms thus act on multiple coupled scales, which have to be retained for a comprehensive picture of the dissipation range in Alfvénic turbulence.
description: Made several improvements to figures and text suggested by referees
(2004) Joseph Stead
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
A Raf-independent Epidermal Growth Factor Receptor Autocrine Loop Is Necessary for Ras Transformation of Rat Intestinal Epithelial Cells
Creators: name: Gangarosa, Lisa M.
name: Graves-Deal, Ramona
name: Oldham, Sean M.
name: Coffey, Robert J.
name: Sizemore, Nywana
name: Der, Channing J.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1997

description: We recently have shown that activated Ras, but not Raf, causes transformation of intestinal (RIE-1, IEC-6) epithelial cells, whereas both activated Ras and Raf transform NIH 3T3 fibroblasts (Oldham, S. M., Clark, G. J., Gangarosa, L. M., Coffey, R. J., and Der, C. J. (1996) Proc. Natl. Acad. Sci. U. S. A. 93, 6924-6928). The observations that conditioned medium from Ras-, but not Raf-, transfected RIE-1 cells, as well as exogenous transforming growth factor alpha (TGFalpha), promoted morphological transformation of parental RIE-1 cells prompted us to identify epidermal growth factor (EGF) receptor (EGFR) ligands produced by Ras-transformed RIE-1 cells responsible for this autocrine effect. Since studies in fibroblasts have shown that v-Src is transforming, we also determined if v-Src could transform RIE-1 cells. H- or K-Ras-transformed cells secreted significant amounts of TGFalpha protein, and mRNA transcripts for TGFalpha, amphiregulin (AR), and heparin-binding EGF-like growth factor (HB-EGF) were induced. Like Ras, v-Src caused morphological and growth transformation of parental RIE-1 cells. However, TGFalpha protein was not secreted by RIE-1 cells stably expressing v-Src or activated Raf, and only minor increases in EGFR ligand mRNA expression were detected in these cells. A selective EGFR tyrosine kinase inhibitor PD153035 attenuated the Ras-, but not Src-, transformed phenotype. Taken together, these observations provide a mechanistic and biochemical basis for the ability of activated Ras, but not activated Raf, to cause transformation of RIE-1 cells. Finally, we suggest that an EGFR-dependent mechanism is necessary for Ras, but not Src, transformation of these intestinal epithelial cells.
Supplemental methods and association results from Kinship influences sperm whale social organization within, but generally not among, social units.
Creators: name: Konrad, Christine M.
name: Gero, Shane
name: Frasier, Timothy
name: Whitehead, Hal
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: 60801 Animal Behaviour

description: Additional details on methods (social association indices, microsatellite genotyping and mtDNA haplotype sequencing; Tables S1 and S2) and on intra-social unit social dynamics (Tables S3 and S4, and Figure S1).
(2005) Martha [Stead]
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Abnormal Glycosylation of Procathepsin L Due to N-terminal Point Mutations Correlates with Failure to Sort to Lysosomes
Creators: name: Chapman, Richard L.
name: Kane, Susan E.
name: Erickson, Ann H.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1997

description: A single point mutation in the lysosomal proenzyme receptor-inhibiting sequence near the N terminus of mouse procathepsin L can result in glycosylation of a normally cryptic site near its C terminus. When alanine replaced His36, Arg38, or Tyr40, the nascent chain of the mutant protein cotranslationally acquired a high mannose oligosaccharide chain at Asn268. In contrast, when alanine replaced Ser34, Arg37, or Leu39, this second carbohydrate chain was not added. This alternating pattern of abnormal glycosylation suggested that propeptide residues 36-40 normally assume an extended conformation having the side chains of residues 36, 38, and 40 facing in the same direction. When tyrosine conservatively replaced His36 or lysine replaced Arg38, Asn268 was not glycosylated. But the procathepsin L mutant having phenylalanine in place of Tyr40 was glycosylated at Asn268, which indicates that the hydrogen bond between the hydroxyl group of Tyr40 and the carboxylate group of Asp82 is necessary for normal folding of the nascent proenzyme chain. Mutation of the adjacent alpha2p (ERININ) helix of the propeptide or addition of a C-terminal epitope tag sequence to procathepsin L also induced misfolding of the proenzyme, as indicated by addition of the second oligosaccharide chain. In contrast, the propeptide mutation KAKK99-102AAAA had no effect on carbohydrate modification even though it reduced the positive charge of the proenzyme. Misfolded mutant mouse procathepsin L was not efficiently targeted to lysosomes on expression in human HeLa cells, even though it acquired phosphate on mannose residues. The majority of the mutant protein was secreted after undergoing modification with complex sugars. Similarly, epitope-tagged mouse procathepsin L was not targeted to lysosomes in homologous mouse cells but was efficiently secreted. Since production of mature endogenous protease was not reduced in cells expressing the tagged protein, the tagged protein did not compete with endogenous procathepsin L for targeting to lysosomes.
Spin correlations and new physics in $τ$-lepton decays at the LHC
Creators: name: Hayreter, Alper
name: Valencia, German
Publisher: name: arXiv
Year: 2015
Subjects: subject: High Energy Physics - Phenomenology (hep-ph)
subject: FOS: Physical sciences

description: We use spin correlations to constrain anomalous $τ$-lepton couplings at the LHC including its anomalous magnetic moment, electric dipole moment and weak dipole moments. Single spin correlations are ideal to probe interference terms between the SM and new dipole-type couplings as they are not suppressed by the $τ$-lepton mass. Double spin asymmetries give rise to $T$-odd correlations useful to probe $CP$ violation purely within the new physics amplitudes, as their appearance from interference with the SM is suppressed by $m_τ$. We compare our constraints to those obtained earlier on the basis of deviations from the Drell-Yan cross-section.
description: 25 pages, 11 figures, 14 tables. v2: added two subsections and relevant discussions, corresponds to version to be published at JHEP
An Intracellular Calcium Signal Activates p70 but Not p90 Ribosomal S6 Kinase in Liver Epithelial Cells
Creators: name: Li, Xiong
name: Lambert, John
name: Hunter, Deborah
name: Graves, Lee M.
name: Earp, H. Shelton
name: He, Yaqin
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1997

description: In the rat liver epithelial cell lines GN4 and WB, angiotensin II (Ang II) activates the Gq class of regulatory G-proteins, increasing intracellular calcium, protein kinase C activity, and protein tyrosine phosphorylation. We compared the ability of Ang II and other compounds that increase intracellular calcium (i.e. the calcium ionophore A23187 and thapsigargin) or protein kinase C activity (the phorbol ester 12-O-tetradecanoylphorbol-13-acetate) to activate p70 ribosomal S6 kinase (p70(S6K)) and p90 ribosomal S6 kinase (p90(RSK)). In GN4 cells, increasing intracellular calcium stimulated p70(S6K) activity in a rapamycin- and wortmannin- sensitive manner, but did not affect p90(RSK) activity. In contrast, 12-O-tetradecanoylphorbol-13-acetate strongly activated p90(RSK) but only weakly stimulated p70(S6K). The ability of calcium to activate p70(S6K) was confirmed by blocking the A23187-dependent activation through chelation of extracellular calcium with EGTA; the effect of thapsigargin was inhibited by the cell permeant chelator bis-(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetraacetoxymethyl ester (BAPTA-AM). Similarly, BAPTA-AM prevented the activation of p70(S6K) by Ang II, suggesting that this signal was largely calcium-dependent. In contrast, the Ang II-dependent activation of mitogen-activated protein kinase and p90(RSK) was not inhibited but was enhanced by BAPTA-AM. These results show that in GN4 cells, Ang II selectively activates p70(S6K) through effects on calcium, p90(RSK) through effects on protein kinase C. The activation of p70(S6K) by calcium stimuli or Ang II was independent of calmodulin but correlated well with the activation of the recently identified, nonreceptor calcium-dependent tyrosine kinase (CADTK)/PYK-2. Both calcium- and Ang II-dependent activation of p70(S6K) were attenuated by the tyrosine kinase inhibitor genistein, and activation of p70(S6K) was higher in GN4 than WB cells, correlating with the increased expression and activation of CADTK/PYK-2 in GN4 cells. In summary, these results demonstrate that intracellular calcium selectively activates p70(S6K) in GN4 cells, consistent with increased CADTK/PYK-2 signaling in these cells.
Research in the Digital Transformation or the Library System
Creators: name: Nagy, Andor
Publisher: name: Bobcatsss
Year: 2023

description: Libraries have always tried to adapt to changing social needs, the development of the sciences, and the emergence of new technological solutions. Consider, for example, the invention of book printing or the mass distribution of printed products and the resulting current bibliographies or even to the gradually emerging reference service. Undoubtedly, the next major stage in the development of library science will be induced by the spread of computer science and informatics. More and more areas of life are digitizing and people are increasingly relying on electronic sources to obtain information thus, the use of digital remotely accessible services has become commonplace. All of this involves a transformation in people’s expectations of the library, and libraries are trying to keep up with emerging needs. More and more libraries offer thematic collections that are available electronically so eventually the digital, remote access use of services become daily. They also enable electronic document lending, online events, e-learning courses and other services that have recently been integrated into the library’s service offering. In my research, I would like to explore how the Hungarian, European and US librarian profession has reacted to the fundamental transformation of the societal demands placed on libraries in the last two decades whether their electronic services have been able to respond appropriately to new societal needs and are still able to meet the role set out in the 1994 IFLA and UNESCO Manifesto on Public Libraries. The aim of my research is to review the transformation of the library service offer over the last two decades, to compare the development direction of Hungarian libraries with international (European and US) trends, and to use new statistics, research results and needs assessments bypass as well as the extent to which libraries have been able to adapt to significantly changed user needs and whether their newly developed services are in line with them.
description: Bobcatsss, Bobcatsss 2021: Digital Transformation
(2001) John Bare
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
(1989) Thomas Larking
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Supplementary Table 3 from The contributions of ankle, knee and hip joint work to individual leg work change during uphill and downhill walking over a range of speeds.
Creators: name: Montgomery, Jana R.
name: Grabowski, Alena M.
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: 110601 Biomechanics
subject: FOS: Health sciences

description: Average (S.D.) hip joint biomechanics for all subjects walking 1.00 m/s, 1.25 m/s, and 1.50 m/s on slopes of −9° to +9°.
Androgen-dependent Protein Interactions within an Intron 1 Regulatory Region of the 20-kDa Protein Gene
Creators: name: French, Frank S.
name: Avellar, Maria Christina W.
name: Gregory, Christopher W.
name: Power, Stephen G.A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1997

description: The 20-kDa protein gene is androgen regulated in rat ventral prostate. Intron 1 contains a 130-base pair complex response element (D2) that binds androgen (AR) and glucocorticoid receptor (GR) but transactivates only with AR in transient cotransfection assays in CV1 cells using the reporter vector D2-tkCAT. To better understand the function of this androgen-responsive unit, nuclear protein interactions with D2 were analyzed by DNase I footprinting in ventral prostate nuclei of intact or castrated rats and in vitro with ventral prostate nuclear protein extracts from intact, castrated, and testosterone-treated castrated rats. Multiple androgen-dependent protected regions and hypersensitive sites were identified in the D2 region with both methods. Mobility shift assays with 32P-labeled oligonucleotides spanning D2 revealed specific interactions with ventral prostate nuclear proteins. Four of the D2-protein complexes decreased in intensity within 24 h of castration. UV cross-linking of the androgen-dependent DNA binding proteins identified protein complexes of approximately 140 and 55 kDa. The results demonstrate androgen-dependent nuclear protein-DNA interactions within the complex androgen response element D2.
Arginine 200 of Heparin Cofactor II Promotes Intramolecular Interactions of the Acidic Domain: IMPLICATION FOR THROMBIN INHIBITION
Creators: name: Monroe, Dougald M.
name: Ciaccia, Angelina V.
name: Church, Frank C.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1997

description: Heparin cofactor II (HCII) is presumed to be a physiological inhibitor of the serine proteinase thrombin. The reaction between HCII and thrombin is quite unique, because it involves an unusual HCII-reactive site loop sequence of Leu444-Ser445, requires the presence of glycosaminoglycans for optimal activity and involves a protein-protein interaction besides the reactive site loop-active site interaction characteristic of serine proteinase inhibitor-serine proteinase pairs. Two mutations at a unique HCII residue, Arg200 --> Ala or Glu, were generated by site-directed mutagenesis. The mutations did not alter either HCII binding to heparin-Sepharose or HCII inhibition of thrombin in the presence of heparin or dermatan sulfate, suggesting that Arg200 is not part of the glycosaminoglycan binding site of HCII. In the absence of glycosaminoglycan, there was a significant increase in alpha-thrombin inhibition by the Arg200 mutants as compared with wild type recombinant HCII (wt-rHCII), whereas inhibition rates with chymotrypsin were identical. Inhibition of gammaT-thrombin, which lacks anion-binding exosite 1 ((ABE-1), the region of alpha-thrombin that interacts with the acidic domain of HCII), was significantly reduced compared with alpha-thrombin, but the reduction was more dramatic for the Arg200-rHCII mutants. Hirugen, which binds to ABE-1 of alpha-thrombin, also diminished inhibition of alpha-thrombin by the Arg200-rHCII mutants to nearly wt-rHCII levels. Both Arg200-rHCII mutants had significantly increased ka values as compared with wt-rHCII, whereas the kd rates were unchanged. Collectively, these results suggest that the improved inhibitory activity of the Arg200-rHCII mutants is mediated by enhanced interactions between the acidic domain and ABE-1, resulting in an increased HCII-thrombin association rate.
(1987) Patrick Larking
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Binding of Upstream Stimulatory Factor to an E-box in the 3′-Flanking Region Stimulates α1(I) Collagen Gene Transcription
Creators: name: Umezawa, Akihiro
name: Brenner, David A.
name: Breindl, Michael
name: Kimball, Jon P.
name: Rippe, Richard A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1997

description: Since several lines of evidence implicate the 3'-flanking region in regulating alpha1(I) collagen gene transcription, we analyzed 12. 4-kilobase pairs of 3'-flanking sequence of the murine alpha1(I) collagen gene for transcriptional elements. A region of the 3'-flanking region stimulated expression of the heterologous beta-globin gene promoter in an enhancer trap plasmid and of the alpha1(I) collagen gene promoter in a collagen-luciferase reporter gene construct when located 3' to the luciferase reporter gene. DNase I footprinting analysis demonstrated the presence of three regions where DNA binding proteins specifically interact within this 3'-stimulatory region. Inspection of the DNA sequence revealed a consensus E-box, a binding site for basic helix-loop-helix proteins, in one of the protein binding sites. Mobility shift assays demonstrated that upstream stimulatory factors (USF) USF-1 and USF-2 bind to this E-box. Mutating the E-box in the context of the 3'-flanking region confirmed that it contributes to the enhancement of transcriptional activity of the alpha1(I) collagen gene promoter. Mutations in all three protein binding sites abolished transcriptional activation by the 3'-flanking region, suggesting a complex interaction among the trans-acting factors in enhancing transcriptional activity. Thus, a region of the 3'-flanking region of the alpha1(I) collagen gene stimulates transcription of the alpha1(I) collagen gene promoter, and USF-1 and USF-2 contribute to this transcriptional stimulation.