The Zoonotic Potential of Canine Influenza Virus: Genetic Adaptations Required for Human Host Infection
Creators: name: Hussain, Zahid
Publisher: name: Zenodo
Year: 2024
Subjects: The Zoonotic Potential
Canine Influenza Virus:
Genetic Adaptations Required for
Human Host Infection

description:  

AbstractCanine Influenza Virus (CIV), an influenza A virus, possesses the capacity for zoonotic transmission. Similar to other influenza viruses that have crossed species barriers, CIV requires specific genetic adaptations to infect humans. These include mutations in the hemagglutinin (HA) protein to optimize receptor binding, alterations in polymerase complex genes for efficient replication, and changes in the non-structural protein (NS1) to evade the human immune response. This review outlines the key factors and mutations necessary for CIV’s adaptation to humans, compares CIV with other zoonotic influenza strains, and estimates the timeline for such adaptations under varying conditions. Enhanced monitoring and genetic analysis are essential to mitigate potential zoonotic risks posed by CIV.




IntroductionThe emergence of zoonotic influenza viruses, such as H5N1 and H7N9, underscores the potential for animal influenza viruses to infect humans and cause pandemics. Canine Influenza Virus (CIV), an influenza A virus primarily infecting dogs, has drawn attention due to its genetic and phenotypic characteristics that mirror other zoonotic influenza viruses. This article explores the genetic adaptations required for CIV to infect humans, focusing on receptor specificity, polymerase activity, and immune evasion mechanisms. Additionally, the article estimates the number of mutations needed and the timeframe for CIV’s potential adaptation to human hosts.




Key Factors for Host Adaptation



Receptor Specificity



CIV binds to α2,3-linked sialic acids, commonly found in avian species and present in the respiratory tracts of dogs.

Human influenza viruses preferentially bind to α2,6-linked sialic acids in the human upper respiratory tract (Rogers et al., 1983).

Mutations in the HA protein are critical for the shift in receptor binding specificity from α2,3 to α2,6-linked sialic acids.


Polymerase Complex Activity



Efficient replication in human cells requires mutations in the polymerase basic 2 (PB2) and polymerase acidic (PA) genes (Mehle & Doudna, 2009).

These mutations enhance viral replication at lower temperatures, such as those in the human upper respiratory tract.


Immune Evasion



The non-structural (NS1) protein plays a pivotal role in counteracting the host’s immune response.

Specific mutations in NS1 can inhibit the production of interferons, allowing the virus to evade the innate immune response (Hale et al., 2008).






Mutations Required for Human Adaptation



Hemagglutinin (HA) ProteinThe HA protein determines receptor binding and cell entry, making it a key factor in host adaptation. Several mutations are necessary for CIV to bind to human-like receptors:



Residue 190 (H3 numbering): Mutation from Glu (E) to Lys (K) improves binding to α2,6-linked sialic acids (Stevens et al., 2006).

Residue 226 (H3 numbering): Mutation from Gln (Q) to Leu (L) enhances receptor specificity for humans.

Residue 228 (H3 numbering): Mutation from Gly (G) to Ser (S) further optimizes binding affinity to human-like receptors.


Polymerase ComplexThe polymerase complex’s ability to replicate efficiently in human cells is influenced by the following mutations:



PB2 Protein:



Position 627 (Glu to Lys): Facilitates replication at the cooler temperatures of the human upper respiratory tract (Mehle & Doudna, 2009).

Position 701 (Asp to Asn): Improves polymerase activity and replication in human cells.


PA Protein:



Position 97 (Asp to Asn): Enhances replication efficiency in mammalian hosts.



Non-Structural (NS1) ProteinThe NS1 protein is essential for immune evasion. Specific mutations include:



Position 42 (Pro to Ser): Inhibits interferon responses, supporting viral replication in human cells (Hale et al., 2008).

Position 138 (Asp to Glu): Alters interactions with host immune factors, aiding in immune system evasion.






Experimental and Observational Evidence



H3N8 CIV



Originating from equine influenza, H3N8 CIV retains characteristics conducive to adaptation, such as partial binding to α2,6-linked sialic acids (Payungporn et al., 2008).


H3N2 CIV



Derived from avian influenza, H3N2 CIV exhibits a stronger affinity for α2,6-linked receptors compared to H3N8, indicating fewer mutations might be required for human adaptation (Song et al., 2008).


Comparative Analysis



H5N1 (Avian Influenza): Requires 5–7 mutations for human adaptation and efficient transmission.

H7N9 (Avian Influenza): Studies identify 4–6 critical mutations for human receptor binding and replication (Herfst et al., 2012).

CIV: An estimated 5–10 mutations across HA, PB2, PA, and NS1 proteins may enable CIV to adapt to humans.






Estimated Timeframe for Adaptation



Without Selection Pressure



In the absence of sustained human exposure, random mutations accumulate over decades to centuries.


Under Selection Pressure



Frequent exposure to humans, such as through close contact with infected dogs, could lead to adaptation within 5–20 years (Parrish & Kawaoka, 2005).


Experimental Evolution



Laboratory studies, such as serial passage in human cells, could yield the necessary mutations within months to a few years (Tumpey et al., 2007).






DiscussionThe genetic plasticity of CIV, combined with its widespread presence in dogs, makes it a plausible candidate for zoonotic transmission. The relatively small number of mutations required for adaptation highlights the importance of active surveillance in canine and human populations. Lessons from H5N1 and H7N9 demonstrate the rapidity with which influenza viruses can adapt under selective pressures. The role of intermediate hosts, environmental factors, and human-animal interactions must also be considered when assessing the risk of CIV becoming a human pathogen.




Conclusion

CIV poses a potential zoonotic risk due to its capacity for genetic adaptation. Key mutations in the HA protein (positions 190, 226, and 228), PB2 protein (positions 627 and 701), PA protein (position 97), and NS1 protein (positions 42 and 138) are critical for adaptation to human hosts. Under natural conditions, this process may take decades, but close contact with humans or experimental evolution could accelerate this timeline to a few years. Continuous surveillance and genetic analysis are essential to prevent potential outbreaks and safeguard public health.




References