title: ÐÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð·Ð½Ð°ÑÐ¸Ð¼Ð¾ÑÑÐ¸ Ð½ÐµÐ¸Ð½Ð²Ð°Ð·Ð¸Ð²Ð½Ð¾Ð³Ð¾ Ð¼ÐµÑÐ¾Ð´Ð° Ð²ÑÑÐ²Ð»ÐµÐ½Ð¸Ñ Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð° Ð² Ð¼Ð¾ÑÐµ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Determining the diagnostic significance of a non-invasive method for detecting amyloid in urine
Creators: name: ÐÐ½Ð¿Ð¸Ð»Ð¾Ð²Ð°, ÐÐ½Ð°ÑÑÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð¾Ð·
ÑÐµÐ½Ð°Ð»ÑÐ½ÑÐ¹ Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð¾Ð·
AL-Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð¾Ð·
AA-Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð¾Ð·
Ð¿Ð¾ÑÐºÐ¸
ÐºÐ¾Ð½Ð³Ð¾ ÐºÑÐ°ÑÐ½ÑÐ¹
Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð½ÑÐµ ÑÐ¸Ð±ÑÐ¸Ð»Ð»Ñ
Ð½ÐµÐ¿ÑÐ°Ð²Ð¸Ð»ÑÐ½ÑÐ¹ ÑÐ¾Ð»Ð´Ð¸Ð½Ð³ Ð±ÐµÐ»ÐºÐ°
Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐ°
ÑÐºÑÐ¸Ð½Ð¸Ð½Ð³
Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð½ÑÐ¹ ÐºÑÐ°ÑÐ¸ÑÐµÐ»Ñ
amyloidosis
amyloid fibril
protein misfolding
renal amyloidosis
AL-amyloidosis
AA-amyloidosis
kidney
congo red
diagnosis
screening
amyloid dye

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«ÐÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð·Ð½Ð°ÑÐ¸Ð¼Ð¾ÑÑÐ¸ Ð½ÐµÐ¸Ð½Ð²Ð°Ð·Ð¸Ð²Ð½Ð¾Ð³Ð¾ Ð¼ÐµÑÐ¾Ð´Ð° Ð²ÑÑÐ²Ð»ÐµÐ½Ð¸Ñ Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð° Ð² Ð¼Ð¾ÑÐµÂ». ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾ÑÑÐ¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ñ Ð½ÐµÐ¸Ð½Ð²Ð°Ð·Ð¸Ð²Ð½Ð¾Ð³Ð¾ ÑÐµÑÑÐ° Ð½Ð° Ð¾ÐºÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ðµ Ð±ÐµÐ»ÐºÐ¾Ð² Ð¼Ð¾ÑÐ¸ Ð¿Ð°ÑÐ¸ÐµÐ½ÑÐ¾Ð² Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´-ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐµÑÐºÐ¸Ð¼ ÐºÑÐ°ÑÐ¸ÑÐµÐ»ÐµÐ¼ ÐÐ¾Ð½Ð³Ð¾ ÐºÑÐ°ÑÐ½ÑÐ¼ Ð´Ð»Ñ Ð¿Ð¾ÑÑÐ°Ð½Ð¾Ð²ÐºÐ¸ Ð´Ð¸Ð°Ð³Ð½Ð¾Ð·Ð° Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð¾Ð· Ð¿Ð¾ÑÐµÐº. ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: 1. ÐÑÑÑÐµÑÑÐ²Ð¸ÑÑ ÑÐ±Ð¾Ñ Ð¸ Ð¿ÑÐ¾Ð±Ð¾Ð¿Ð¾Ð´Ð³Ð¾ÑÐ¾Ð²ÐºÑ Ð¾Ð±ÑÐ°Ð·ÑÐ¾Ð² Ð¼Ð¾ÑÐ¸ Ð¿Ð°ÑÐ¸ÐµÐ½ÑÐ¾Ð² Ñ Ð´Ð¸Ð°Ð³Ð½Ð¾Ð·Ð¾Ð¼ Ð½ÐµÑÑÐ¾Ð¿Ð°ÑÐ¸Ñ Ð¸ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½Ð¾Ð¹ Ð³ÑÑÐ¿Ð¿Ñ. 2. ÐÑÐ¾Ð°Ð½Ð°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°ÑÑ ÑÐ¾Ð±ÑÐ°Ð½Ð½ÑÐµ Ð¾Ð±ÑÐ°Ð·ÑÑ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ CRD-ÑÐµÑÑÐ° Ð¸ ÑÐ°ÑÑÑÐ¸ÑÐ°ÑÑ ÐºÐ¾ÑÑÑÐ¸ÑÐ¸ÐµÐ½Ñ ÑÐ´ÐµÑÐ¶Ð°Ð½Ð¸Ñ ÐºÑÐ°ÑÐ¸ÑÐµÐ»Ñ ÐÐ¾Ð½Ð³Ð¾ ÐºÑÐ°ÑÐ½Ð¾Ð³Ð¾ Ð½Ð° Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ðµ (CRR). 3. ÐÐ¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ Ð¿Ð¾ÑÐ¾Ð³Ð¾Ð²Ð¾Ðµ Ð·Ð½Ð°ÑÐµÐ½Ð¸Ðµ Ð¿Ð¾ÐºÐ°Ð·Ð°ÑÐµÐ»Ñ CRR, ÑÐºÐ°Ð·ÑÐ²Ð°ÑÑÐµÐ³Ð¾ Ð½Ð° Ð½Ð°Ð»Ð¸ÑÐ¸Ðµ Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð¾Ð·Ð°. 4. ÐÑÐ¾Ð²ÐµÑÑÐ¸ ÑÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÑÑ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÑ Ð´Ð°Ð½Ð½ÑÑ Ð´Ð»Ñ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐ½Ð¾ÑÑÐ¸ Ð¼ÐµÑÐ¾Ð´Ð¸ÐºÐ¸. 5. ÐÑÐ¾Ð²ÐµÑÑÐ¸ Ð¾ÐºÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ðµ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð² Ð¼Ð¾ÑÐ¸ ÐÐ¾Ð½Ð³Ð¾ ÐºÑÐ°ÑÐ½ÑÐ¼ Ñ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐµÐ¹ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸ÐµÐ¹ Ð² Ð¿Ð¾Ð»ÑÑÐ¸Ð·Ð¾Ð²Ð°Ð½Ð½Ð¾Ð¼ ÑÐ²ÐµÑÐµ. Ð Ð°Ð±Ð¾ÑÐ° Ð±ÑÐ»Ð° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð² Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ Ð±Ð¸Ð¾ÑÐ¸Ð¼Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð³Ð¾Ð¼ÐµÐ¾ÑÑÐ°Ð·Ð° ÐÐÐ ÐÐµÑÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¤ÐÐÐÐ£ ÐÐ ÐÐ¡ÐÐ±ÐÐÐ£ Ð¸Ð¼. Ð°Ðº. Ð.Ð. ÐÐ°Ð²Ð»Ð¾Ð²Ð° ÐÐ¸Ð½Ð·Ð´ÑÐ°Ð²Ð° Ð Ð¾ÑÑÐ¸Ð¸ Ð¸ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ð¸ Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð¾Ð² Ð¡ÐÐ±ÐÐ£. ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¾Ð´Ð¾Ð±ÑÐµÐ½Ð¾ Ð»Ð¾ÐºÐ°Ð»ÑÐ½ÑÐ¼ ÑÑÐ¸ÑÐµÑÐºÐ¸Ð¼ ÐºÐ¾Ð¼Ð¸ÑÐµÑÐ¾Ð¼ ÐÐ¡ÐÐ±ÐÐÐ£. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð¿ÑÐ¾Ð´ÐµÐ»Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¸ ÑÐ¾Ð±ÑÐ°Ð½Ñ Ð¾Ð±ÑÐ°Ð·ÑÑ Ð¿Ð°ÑÐ¸ÐµÐ½ÑÐ¾Ð² Ñ Ð´Ð¸Ð°Ð³Ð½Ð¾Ð·Ð¾Ð¼ Ð½ÐµÑÑÐ¾Ð¿Ð°ÑÐ¸Ñ (Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð¾Ð· Ð¿Ð¾ÑÐµÐº Ð¸ Ð½ÐµÐ°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð½ÑÐ¹ Ð²Ð°ÑÐ¸Ð°Ð½Ñ Ð½ÐµÑÑÐ¾Ð¿Ð°ÑÐ¸Ð¸) Ð¸ Ð¿ÑÐ¾Ð°Ð½Ð°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°Ð½Ñ Ð² Ð´Ð²ÑÑ Ð½ÐµÐ·Ð°Ð²Ð¸ÑÐ¸Ð¼ÑÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ñ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ CRD-ÑÐµÑÑÐ°. ÐÐ»Ñ ÐºÐ°Ð¶Ð´Ð¾Ð³Ð¾ Ð¾Ð±ÑÐ°Ð·ÑÐ° Ð±ÑÐ» ÑÐ°ÑÑÑÐ¸ÑÐ°Ð½ Ð¿Ð¾ÐºÐ°Ð·Ð°ÑÐµÐ»Ñ CRR â ÐºÐ¾ÑÑÑÐ¸ÑÐ¸ÐµÐ½Ñ ÑÐ´ÐµÑÐ¶Ð°Ð½Ð¸Ñ ÐºÑÐ°ÑÐ¸ÑÐµÐ»Ñ ÐÐ¾Ð½Ð³Ð¾ ÐºÑÐ°ÑÐ½Ð¾Ð³Ð¾ Ð½Ð° Ð½Ð¸ÑÑÐ¾ÑÐµÐ»Ð»ÑÐ»Ð¾Ð·Ð½Ð¾Ð¹ Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ðµ. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð±ÑÐ»Ð¾ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¾ Ð¿Ð¾ÑÐ¾Ð³Ð¾Ð²Ð¾Ðµ Ð·Ð½Ð°ÑÐµÐ½Ð¸Ðµ Ð¿Ð¾ÐºÐ°Ð·Ð°ÑÐµÐ»Ñ CRR, ÑÐºÐ°Ð·ÑÐ²Ð°ÑÑÐµÐµ Ð½Ð° Ð½Ð°Ð»Ð¸ÑÐ¸Ðµ Ð´Ð¸Ð°Ð³Ð½Ð¾Ð·Ð° Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð¾Ð· Ð¿Ð¾ÑÐµÐº Ð¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¾ Ð¾ÐºÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ðµ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð² Ð¼Ð¾ÑÐ¸ ÐÐ¾Ð½Ð³Ð¾ ÐºÑÐ°ÑÐ½ÑÐ¼ Ñ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐµÐ¹ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸ÐµÐ¹ Ð² Ð¿Ð¾Ð»ÑÑÐ¸Ð·Ð¾Ð²Ð°Ð½Ð½Ð¾Ð¼ ÑÐ²ÐµÑÐµ, Ð¿Ð¾Ð´ÑÐ²ÐµÑÐ¶Ð´Ð°ÑÑÐµÐ¹ Ð½Ð°Ð»Ð¸ÑÐ¸Ðµ Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð½ÑÑ Ð°Ð³ÑÐµÐ³Ð°ÑÐ¾Ð² Ð² Ð¾Ð±ÑÐ°Ð·ÑÐ°Ñ Ð¼Ð¾ÑÐ¸.
description: The subject of the graduate qualification work is Â«Determining the diagnostic significance of a non-invasive method for detecting amyloid in urineÂ». The given work is devoted to studying of the possibility of using a non-invasive test for staining the urine proteins of patients with amyloid-specific dye Congo red for the diagnosis of kidney amyloidosis. The research set the following goals: 1. To carry out the collection and sample preparation urine samples from patients with a diagnosis of nephropathy and the control group. 2. Analyze the collected samples using a CRD test and calculate the retention coefficient of Congo red dye on the membrane (CRR). 3. Determine the range of values of the CRR indicator indicating the presence of amyloidosis. 4. Perform statistical data processing to determine the specificity of the method. 5. Perform staining of urine samples with Congo red followed by microscopy in polarized light. The work was performed in the laboratory of biochemical homeostasis research Institute of Nephrology of First Pavlov State Medical University of St. Petersburg and laboratory of amyloid biology, St. Petersburg State University. The study was approved by the local ethics Committee of SPbGMU. As a result of this work, samples of patients diagnosed with nephropathy (amyloidosis of the kidneys and non-amyloid variant of nephropathy) were collected and analyzed in two independent experiments using a CRD-test. For each sample, a CRR indicator was calculated â the retention coefficient of the Congo red dye on the nitrocellulose membrane. As a result, the threshold value of the CRR indicator was determined, indicating the presence of a diagnosis of kidney amyloidosis, and the urine samples were stained Congo red with subsequent microscopy in polarized light, confirming the presence of amyloid aggregates in the urine samples.