Osteogenic differentiation of human mesenchymal stem cells in 3-D Zr-Si organic-inorganic scaffolds produced by two-photon polymerization technique
Creators: name: Koroleva, Anastasia
name: Deiwick, Andrea
name: Nguyen, Alexander
name: Schlie-Wolter, Sabrina
name: Narayan, Roger
name: Timashev, Peter
name: Popov, Vladimir
name: Bagratashvili, Victor N.
name: Chichkov, Boris
Publisher: name: San Francisco, CA : Public Library of Science
Year: 2015
Subjects: subject: alcohol
subject: alkaline phosphatase
subject: calcium ion
subject: molecular scaffold
subject: osteocalcin
subject: polymer
subject: silicon
subject: zirconium
subject: calcium
subject: inorganic compound
subject: organic compound
subject: adipose derived stem cell
subject: aging
subject: aqueous solution
subject: Article
subject: bone development
subject: bone marrow derived mesenchymal stem cell
subject: bone matrix
subject: bone mineralization
subject: bone tissue
subject: calcium bone level
subject: calcium cell level
subject: cell differentiation
subject: cell labeling
subject: cell lineage
subject: composite material
subject: controlled study
subject: culture medium
subject: hardness
subject: human
subject: human cell
subject: mesenchymal stem cell
subject: microtechnology
subject: ossification
subject: polymerization
subject: scanning electron microscopy
subject: staining
subject: stem cell culture
subject: synthesis
subject: tissue engineering
subject: tissue scaffold
subject: two photon polymerization
subject: Young modulus
subject: adipose tissue
subject: cell culture
subject: cell culture technique
subject: cell proliferation
subject: chemistry
subject: cytology
subject: mesenchymal stroma cell
subject: metabolism
subject: procedures
subject: reproducibility
subject: stem cell
subject: time factor
subject: ultrastructure
subject: Adipose Tissue
subject: Alkaline Phosphatase
subject: Calcium
subject: Cell Culture Techniques
subject: Cell Differentiation
subject: Cell Proliferation
subject: Cells, Cultured
subject: Chemistry Techniques, Synthetic
subject: Elastic Modulus
subject: Hardness
subject: Humans
subject: Inorganic Chemicals
subject: Mesenchymal Stromal Cells
subject: Microscopy, Electron, Scanning
subject: Organic Chemicals
subject: Osteocalcin
subject: Osteogenesis
subject: Polymerization
subject: Reproducibility of Results
subject: Silicon
subject: Stem Cells
subject: Time Factors
subject: Tissue Engineering
subject: Tissue Scaffolds
subject: Zirconium
subject: Dewey Decimal Classification::500 | Naturwissenschaften
subject: Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit

description: Two-photon polymerization (2PP) is applied for the fabrication of 3-D Zr-Si scaffolds for bone tissue engineering. Zr-Si scaffolds with 150, 200, and 250 μm pore sizes are seeded with human bone marrow stem cells (hBMSCs) and human adipose tissue derived stem cells (hASCs) and cultured in osteoinductive and control media for three weeks. Osteogenic differentiation of hASCs and hBMSCs and formation of bone matrix is comparatively analyzed via alkaline phosphatase activity (ALP), calcium quantification, osteocalcin staining and scanning electron microscopy (SEM). It is observed that the 150 μm pore size Zr-Si scaffolds support the strongest matrix mineralization, as confirmed by calcium deposition. Analysis of ALP activity, osteocalcin staining and SEM observations of matrix mineralization reveal that mesenchymal stem cells cultured on 3-D scaffolds without osteogenic stimulation spontaneously differentiate towards osteogenic lineage. Nanoindentation measurements show that aging of the 2PP-produced Zr-Si scaffolds in aqueous or alcohol media results in an increase in the scaffold Young's modulus and hardness. Moreover, accelerated formation of bone matrix by hASCs is noted, when cultured on the scaffolds with lower Young's moduli and hardness values (non aged scaffolds) compared to the cells cultured on scaffolds with higher Young's modulus and hardness values (aged scaffolds). Presented results support the potential application of Zr-Si scaffolds for autologous bone tissue engineering.