title: Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° ÑÐ¿Ð¾ÑÐ¾Ð±Ð° Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð½Ð¾Ð³Ð¾ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Vischeria punctate Ð´Ð»Ñ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ, Ð¾Ð±Ð¾Ð³Ð°ÑÐµÐ½Ð½Ð¾Ð¹ ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ð°Ð¼Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Develoment of a method for targeted cultivation of Vischeria punctate to produce biomass enriched with carotenoids
Creators: name: ÐÑÑÑÐ°ÑÐ¾ÐºÑÐ»Ð¾Ð², ÐÐ°ÑÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ñ
Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð½Ð¾Ðµ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
Ð±Ð¸Ð¾Ð¼Ð°ÑÑÐ°
ÑÐºÑÑÑÐ°ÐºÑÐ¸Ñ
carotenoids
targeted cultivation
biomass
extraction

description: Ð ÑÐ°Ð±Ð¾ÑÐµ Ð¿Ð¾Ð´Ð±Ð¸ÑÐ°ÑÑÑÑ ÑÑÐ»Ð¾Ð²Ð¸Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Vischeria punctate, ÑÑÐ¸Ð¼ÑÐ»Ð¸ÑÑÑÑÐ¸Ðµ ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð³ÐµÐ½ÐµÐ· Ð¸ Ð¿ÑÐµÐ´Ð»Ð°Ð³Ð°ÐµÑÑÑ ÑÐ¿Ð¾ÑÐ¾Ð± Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð¾Ð±Ð¾Ð³Ð°ÑÐµÐ½Ð½Ð¾Ð¹ ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ð°Ð¼Ð¸. ÐÐ±ÑÐµÐºÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ñ Vischeria punctata Ð¸Ð· Ð¸Ð· ÐºÐ¾Ð»Ð»ÐµÐºÑÐ¸Ð¸ ÐÐ¤Ð£ Ð¸Ð¼. Ð. ÐÐ°Ð½ÑÐ°, Ð¼Ð°ÑÐºÐ¸ÑÐ¾Ð²ÐºÐ° ÑÑÐ°Ð¼Ð¼Ð° IPPAS H-242 (ÐÐ¤Ð  Ð ÐÐ Ð¸Ð¼. Ð.Ð.Ð¢Ð¸Ð¼Ð¸ÑÑÐ·ÐµÐ²Ð°). ÐÐ°Ð´Ð°ÑÐ¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: - Ð¿Ð¾Ð´Ð¾Ð±ÑÐ°ÑÑ Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½ÑÑ ÑÑÐµÐ´Ñ Ð´Ð»Ñ Ð¼Ð°ÐºÑÐ¸Ð¼Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð½Ð°ÐºÐ¾Ð¿Ð»ÐµÐ½Ð¸Ñ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹, Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÐ¾Ð² ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¸ Ð²ÑÑÐ¾Ð´ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ; - Ð¾Ð¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ Ð¾Ð¿ÑÐ¸Ð¼Ð°Ð»ÑÐ½ÑÑ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ñ Ð²Ð½Ð¾ÑÐ¸Ð¼Ð¾Ð¹ Ð² ÑÑÑÐ¿ÐµÐ½Ð·Ð¸Ñ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ð¸ Ð¿ÐµÑÐµÐºÐ¸ÑÐ¸ Ð²Ð¾Ð´Ð¾ÑÐ¾Ð´Ð° Ð´Ð»Ñ ÑÑÐ¸Ð¼ÑÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð³ÐµÐ½ÐµÐ·Ð°, - Ð¾Ð¿Ð¸ÑÐ°ÑÑ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÑ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ð¸, Ð¾ÑÐµÐ½Ð¸ÑÑ ÑÐ¾ÑÑÐ¾ÑÐ½Ð¸Ðµ Ð¿Ð¾Ð¿ÑÐ»ÑÑÐ¸Ð¸ Vischeria punctata Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸ÑÐµÑÐºÐ¸Ð¼ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð¸ Ð¾Ð¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ Ð²ÑÑÐ¾Ð´ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð¿ÑÐ¸ Ð²Ð»Ð¸ÑÐ½Ð¸Ð¸ ÑÑÑÐµÑÑÐ¾Ð²Ð¾Ð³Ð¾ ÑÐ°ÐºÑÐ¾ÑÐ°; - Ð² Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½Ð¾Ð¹ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÐµ Ð¾Ð¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ðµ ÑÐ¾ÑÐ¾ÑÐ¸Ð½ÑÐµÐ·Ð¸ÑÑÑÑÐ¸Ñ Ð¿Ð¸Ð³Ð¼ÐµÐ½ÑÐ¾Ð² Ð¸ ÑÑÐ¼Ð¼Ñ ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ð¾Ð²; Ð Ð°Ð±Ð¾ÑÐ° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ ÐÐ¨ÐÐ¸ÐÐ Ð¡ÐÐ±ÐÐ£. ÐÐ¿ÑÐ¸Ð¼Ð°Ð»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ´Ð¾Ð¹ Ð´Ð»Ñ Ð½Ð°ÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ñ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ð¸ ÑÑÐ°Ð»Ð° ÑÑÐµÐ´Ð° ÐÐ¾Ð»Ð´Ð°. Ð ÐºÐ°ÑÐµÑÑÐ²Ðµ ÑÑÑÐµÑÑÐ¾Ð²Ð¾Ð³Ð¾ ÑÐ°ÐºÑÐ¾ÑÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ Ð¿ÐµÑÐµÐºÐ¸ÑÑ Ð²Ð¾Ð´Ð¾ÑÐ¾Ð´Ð° Ð² ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸ÑÑ Ð¾Ñ 0,05 Ð´Ð¾ 0,15 Ð¼Ð» Ð½Ð° 500 Ð¼Ð» ÑÑÑÐ¿ÐµÐ½Ð·Ð¸Ð¸ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ð¸, ÐºÐ¾ÑÐ¾ÑÑÐ¹ Ð²Ð½Ð¾ÑÐ¸Ð»Ð¸ ÐºÐ°Ð¶Ð´ÑÐµ Ð´Ð²Ð¾Ðµ ÑÑÑÐ¾Ðº Ð² ÑÐµÑÐµÐ½Ð¸Ðµ 10 ÑÑÑÐ¾Ðº ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ. Ð ÑÐ¾Ð¼ Ð¶Ðµ ÑÐµÐ¶Ð¸Ð¼Ðµ Ð²Ð½Ð¾ÑÐ¸Ð»ÑÑ Ð¸ ÑÐ°ÐºÑÐ¾Ñ ÑÐ¾ÑÑÐ° Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹ Ð¿Ð¸ÑÐ¸Ð´Ð¾ÐºÑÐ¸Ð½ (50 Ð¼Ð³ / Ð»). ÐÐ°Ð¸Ð»ÑÑÑÐ¸Ð¼ Ð²Ð°ÑÐ¸Ð°Ð½ÑÐ¾Ð¼ ÑÑÐ°Ð»Ð¾ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ðµ 0,10 Ð¼Ð» Ð¿ÐµÑÐµÐºÐ¸ÑÐ¸ Ð²Ð¾Ð´Ð¾ÑÐ¾Ð´Ð° Ð½Ð° 500 Ð¼Ð» ÑÑÑÐ¿ÐµÐ½Ð·Ð¸Ð¸ V. punctata, Ð¿ÑÐ¸ ÑÑÐ¾Ð¼ Ð²ÑÑÐ¾Ð´ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð´Ð¾ÑÑÐ¾Ð²ÐµÑÐ½Ð¾ Ð½Ðµ Ð¾ÑÐ»Ð¸ÑÐ°Ð»ÑÑ Ð¾Ñ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½Ð¾Ð³Ð¾ Ð²Ð°ÑÐ¸Ð°Ð½ÑÐ°, ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ðµ ÑÑÐ¼Ð¼Ñ ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ð¾Ð² Ð²Ð¾Ð·ÑÐ°ÑÑÐ°ÐµÑ Ð½Ð° 23,1 % Ð¿Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ Ñ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÐµÐ¼ Ð¸ Ð´Ð¾ÑÑÐ¸Ð³Ð°ÐµÑ 5,97Â±0,11 Ð¼Ð³/Ð³ Ð²Ð¾Ð·Ð´ÑÑÐ½Ð¾-ÑÑÑÐ¾Ð¹ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ. Ð ÑÐ°Ð±Ð¾ÑÐµ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ¾Ð²ÑÐµÐ¼ÐµÐ½Ð½ÑÐµ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ: Â«ÐÐ¾Ð»Ð»ÐµÐºÑÐ¸Ñ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹ Ð¸ ÑÐ¸Ð°Ð½Ð¾Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ IPPAS ÐÐ¤Ð  Ð ÐÐÂ», Â«Listing the Worldâs Algae AlgaeBaseÂ»; Â«ÐÐ¾Ð»Ð»ÐµÐºÑÐ¸Ñ ÐºÑÐ»ÑÑÑÑ Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹ Ð² ÐÐµÑÑÐ¸Ð½Ð³ÐµÐ½ÑÐºÐ¾Ð¼ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑÐµ (SAG)Â», Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ Ð¾ÑÐµÑÐµÑÑÐ²ÐµÐ½Ð½Ð¾Ð¹ Ð¸ Ð·Ð°ÑÑÐ±ÐµÐ¶Ð½Ð¾Ð¹ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÑ: ÐLIBRARY, Scopus, ÐÐºÐ°Ð´ÐµÐ¼Ð¸Ñ Google, Ñ ÑÑÐµÑÐ¾Ð¼ Ð¾ÑÐ½Ð¾Ð²Ð½ÑÑ ÑÑÐµÐ±Ð¾Ð²Ð°Ð½Ð¸Ð¹ Ð¸Ð½ÑÐ¾ÑÐ¼Ð°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ Ð±ÐµÐ·Ð¾Ð¿Ð°ÑÐ½Ð¾ÑÑÐ¸. ÐÐ»Ñ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð¸ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÐ¸ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð² Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ñ Microsoft Excel, ÐÐ¡-foto, Levenhuk.
description: The work selects cultivation conditions for Vischeria punctata that stimulate carathogensis and proposes a method for obtaining biomass enriched with carotenoids Objects of study â microalgae Vischeria punctata from the collection I. Kant Baltic Federal University, labeling of the IPPAS H-242 strain (Timiryazev Institute of Plant Physiology of the Russian Academy of Sciences). Research objectives â to select a nutrient medium for maximum accumulation of microalgae biomass, determination of cultivation parameters and biomass yield â to determine the optimal concentration of hydrogen peroxide introduced into the suspension of microalgae to stimulate carotenogenesis, describe the parameters of microalgae cultivation, Â  â to assess the state of the Vischeria punctata population by microscopic method and determine the biomass yield under the influence of a stress factor; â in the resulting biomass, determine the content of photosynthetic pigments and the amount of carotenoids; The work was carried out on the basis of the laboratory Higher School of Biotechnology and Food Production. The optimal environment for increasing microalgae biomass has become the Balda environment. Hydrogen peroxide was used as a stress factor in concentrations from 0.05 to 0.15 ml per 500 ml of microalgae suspension, which was introduced every two days during 10 days of cultivation. In the same mode, the microalgae growth factor pyridoxine (50 mg / l) was introduced. The best option was to use 0.10 ml of hydrogen peroxide per 500 ml of V. punctata suspension, while the biomass yield did not significantly differ from the control variant, the amount of carotenoids increased by 23.1 % compared with the control and reached 5.97Â±0.11 mg/g of air- dry biomass. Modern databases were used in the work: Â«Collection of microalgae and cyanobacteria IPPAS Institute of Plant Physiology of the Russian Academy of SciencesÂ», Â«Listing the Worldâs Algae AlgaeBaseÂ»; Â«Collection of algae cultures at the University of Gottingen (SAG)Â», databases of Russian and foreign literature: ÐLIBRARY, Scopus, Google Academy, taking into account the basic requirements of information security. Microsoft Excel, MS-foto, Levenhuk programs were used to analyze and process the research results.