Additional file 1 of Intrinsic nucleus-targeted ultra-small metal–organic framework for the type I sonodynamic treatment of orthotopic pancreatic carcinoma
Creators: name: Zhang, Tao
name: Sun, Yu
name: Cao, Jing
name: Luo, Jiali
name: Wang, Jing
name: Jiang, Zhenqi
name: Huang, Pintong
Publisher: name: figshare
Year: 2021
Subjects: subject: Space Science
subject: Medicine
subject: Cell Biology
subject: Molecular Biology
subject: Pharmacology
subject: Chemical Sciences not elsewhere classified
subject: Cancer
subject: Plant Biology
subject: FOS: Biological sciences

description: Additional file 1: Fig. S1. Structural formula and synthesis method of ultra-small Ti-TCPP MOF. Fig. S2. TEM images and DLS measurement of Ti-TCPP MOF in PBS for 21 days (a) and FBS for 7 days (b) at 4 ℃. Scale bar: 50 nm. Fig. S3. XRD pattern of Ti-TCPP MOF in PBS for 21 days at 4 ℃. Fig. S4. TEM images and DLS measurement of Ti-TCPP MOF in PBS (a) and FBS for 3 days (b) at 37 ℃. Scale bar: 50 nm. Fig. S5. TEM images and DLS measurement of Ti-TCPP MOF in PBS after US irradiation (0.5 W cm−2, 1 MHz, 50% duty cycle, 1 min). Scale bar: 50 nm. Fig. S6. N2 adsorption/desorption result of Ti-TCPP MOF. Fig. S7. (a) Power-dependent 1O2 generation after US irradiation under normoxia conditions. (b) A standard curve of H2O2 generation measured by multiscan spectrum. Fig. S8. The PA signal of Ti-TCPP MOF under 680–900 nm pulse laser irradiation in vitro. Fig. S9. Cell viability of Panc02 (a) and hTERT-HPNE (b) cells after incubated with Ti-TCPP MOF for 24 h. Fig. S10. Cell viability of BxPC-3 cells after US irradiation (1 MHz, 50% duty cycle, 1 min) for 24 h. Fig. S11. Detection of ROS generation by DCFH-DA kit in tumor cells. Fig. S12. The quantified levels of Bax/Bcl-2 was analyzed by Image J software. The data were expressed as mean ± S.D. (n = 3). ***P &lt; 0.001. Fig. S13. Cell cycle analysis determined by flow cytometry (a) and statistical analysis of sub-G1 phase (b) after treatment with Ti-TCPP + US at different time period (n = 3). ***p &lt; 0.001. Fig. S14. Confocal images of cancer cells in which the nuclei were stained blue with Hoechst and the γ-H2AX foci bright green following nuclear-targeting Ti-TCPP MOF treatment or US irradiation. Fig. S15. Hemolysis coefficient after incubation of RBCs with Ti-TCPP MOF (400, 200, 100, 50, or 25 µg mL−1) or water, respectively (n = 3). The hemolysis coefficient of Ti-TCPP MOF was less than 5%, indicating the good biocompatibility of Ti-TCPP MOF. Fig. S16. The body weight changes of healthy mice treated by PBS or Ti-TCPP MOF during 14 days. Fig. S17. Blood routine analysis. Blood levels of WBC, RBC, HGB, HCT, MCHC, PLT, MCV and MCH of health mice after 14 d post injection (i.v.) of Ti-TCPP MOF, PBS was set as control. Fig. S18. (a) Statistics analysis of tumor fluorescence intensity after i.v. Ti-TCPP MOF injection at different time period (n = 3). (b) Statistics analysis of fluorescence intensity of main organs 12 h after i.v. Ti-TCPP MOF injection (n = 3). Fig. S19. The body weight of mice after various treatment for 15 days, indicating no acute toxicity to mice major organs. n = 5. Fig. S20. Histological analysis of the main organs (heart, liver, spleen, lung and kidney) of un-treated mice (control) and mice treated with US, Ti-TCPP MOF and Ti-TCPP MOF + US for 15 days. Scale bar = 100 µm.