title: ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð¸ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½ÑÑ Ð¼ÐµÑ Ð°Ð½Ð¸Ð·Ð¼Ð¾Ð² Ð¿Ð°ÑÐ¾Ð³ÐµÐ½ÐµÐ·Ð° Ð²Ð¾ÑÐ¿Ð°Ð»Ð¸ÑÐµÐ»ÑÐ½ÑÑ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ð¹ ÐºÐ¸ÑÐµÑÐ½Ð¸ÐºÐ° Ð½Ð° Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð¼Ð¾Ð´ÐµÐ»ÑÑ in vitro Ð¸ in vivo
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Study of cellular and molecular mechanisms of pathogenesis of inflammatory bowel diseases on biological models in vitro and in vivo
Creators: name: ÐÐ°ÑÐ¸Ð½Ð°, Ð¢Ð°ÑÑÑÐ½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð²Ð¾ÑÐ¿Ð°Ð»Ð¸ÑÐµÐ»ÑÐ½ÑÐµ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ñ ÐºÐ¸ÑÐµÑÐ½Ð¸ÐºÐ°
Ð¿Ð¾Ð²ÑÑÐµÐ½Ð½Ð°Ñ Ð¿ÑÐ¾Ð½Ð¸ÑÐ°ÐµÐ¼Ð¾ÑÑÑ ÐºÐ¸ÑÐµÑÐ½Ð¸ÐºÐ°
inflammatory bowel disease
increased intestinal permeability

description: Ð¦ÐµÐ»ÑÑ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÐµÑÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¾ÑÐ½Ð¾Ð²Ð½ÑÑ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð² Ð¿Ð°ÑÐ¾Ð³ÐµÐ½ÐµÐ·Ð° Ð²Ð¾ÑÐ¿Ð°Ð»Ð¸ÑÐµÐ»ÑÐ½ÑÑ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ð¹ ÐºÐ¸ÑÐµÑÐ½Ð¸ÐºÐ° â Ð½Ð°ÑÑÑÐµÐ½Ð¸Ðµ Ð±Ð°ÑÑÐµÑÐ½ÑÑ ÑÑÐ½ÐºÑÐ¸Ð¹ ÑÐ¿Ð¸ÑÐµÐ»Ð¸Ñ Ð¸ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ñ Ð²Ð¾ÑÐ¿Ð°Ð»ÐµÐ½Ð¸Ñ in vitro Ð½Ð° ÐºÑÐ»ÑÑÑÑÐµ ÐºÐ»ÐµÑÐ¾Ðº Caco-2 Ð¸ in vivo Ð½Ð° Ð¼ÑÑÐ°Ñ Ñ Ð½Ð¾ÐºÐ°ÑÑÐ¾Ð¼ Ð³ÐµÐ½Ð° Ð¼ÑÑÐ¸Ð½ 2 (Muc2-/-). ÐÑÐ¸ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð¸Ð¸ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±Ð·Ð¾ÑÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ: PubMed, Scopus, Web of Science, e-library, Cyberleninka, Science Direct, ÐÐºÐ°Ð´ÐµÐ¼Ð¸Ñ Google. Ð¡ÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÑÑ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»Ð¸ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿Ð°ÐºÐµÑÐ° Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼ GraphPadPrism, Statistica. Ð Ð°Ð±Ð¾ÑÐ° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ Ð²ÑÑÑÐµÐ¹ ÑÐºÐ¾Ð»Ñ Ð±Ð¸Ð¾ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¹ Ð¸ Ð¿Ð¸ÑÐµÐ²ÑÑ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´ÑÑÐ² Ð¡ÐÐ±ÐÐ£ Ð¸ Ð½Ð° Ð±Ð°Ð·Ðµ Ð¾ÑÐ´ÐµÐ»Ð¾Ð² Ð±Ð¸Ð¾ÑÐ¸Ð¼Ð¸Ð¸ Ð¸ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¤ÐµÐ´ÐµÑÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð³Ð¾ÑÑÐ´Ð°ÑÑÑÐ²ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð±ÑÐ´Ð¶ÐµÑÐ½Ð¾Ð³Ð¾ Ð½Ð°ÑÑÐ½Ð¾Ð³Ð¾ ÑÑÑÐµÐ¶Ð´ÐµÐ½Ð¸Ñ Â«ÐÐ½ÑÑÐ¸ÑÑÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð¼ÐµÐ´Ð¸ÑÐ¸Ð½ÑÂ». Ð ÑÐ°Ð±Ð¾ÑÐµ Ð½Ð° Ð¼Ð¾Ð´ÐµÐ»ÑÑ in vitro Ð¸ in vivo Ð±ÑÐ»Ð¸ Ð¾ÑÐ¾Ð±ÑÐ°Ð¶ÐµÐ½Ñ Ð¾ÑÐ½Ð¾Ð²Ð½ÑÐµ ÑÐ°ÐºÑÐ¾ÑÑ Ð¿Ð°ÑÐ¾Ð³ÐµÐ½ÐµÐ·Ð° Ð²Ð¾ÑÐ¿Ð°Ð»Ð¸ÑÐµÐ»ÑÐ½ÑÑ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ð¹ ÐºÐ¸ÑÐµÑÐ½Ð¸ÐºÐ°. ÐÑÐ»Ð° Ð¾ÑÐ¼ÐµÑÐµÐ½Ð° Ð¿Ð¾Ð²ÑÑÐµÐ½Ð½Ð°ÑÂ  Ð¿ÑÐ¾Ð½Ð¸ÑÐ°ÐµÐ¼Ð¾ÑÑÑ Ð¸ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ðµ Ð²Ð¾ÑÐ¿Ð°Ð»Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ Ð¾ÑÐ²ÐµÑÐ° Ð½Ð° Ð¼Ð¾Ð´ÐµÐ»ÑÑ ÐÐÐ in vitro, Ð¸ Ð¿ÑÐ¸Ð·Ð½Ð°ÐºÐ¸ Ð²Ð¾ÑÐ¿Ð°Ð»ÐµÐ½Ð¸Ñ Ð½Ð° Ð¼Ð¾Ð´ÐµÐ»Ð¸ in vivo. Ð Ð°ÑÑÐ¼Ð¾ÑÑÐµÐ½Ð½ÑÐµ Ð¼Ð¾Ð´ÐµÐ»Ð¸ ÐÐÐ Ð¾ÑÐ¾Ð±ÑÐ°Ð¶Ð°ÑÑ Ð¿Ð°ÑÐ¾Ð³ÐµÐ½ÐµÐ· Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ñ Ð¸ Ð¼Ð¾Ð³ÑÑ Ð±ÑÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ Ð´Ð»Ñ Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐµÐ³Ð¾ Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð².
description: The aim of this work is to study the main factors of pathogenesis of inflammatory bowel diseases - impaired epithelial barrier functions and inflammation development using in vitro model of of inflammatory bowel disease (Caco-2 cell culture) and in vivo model - mice with mucin 2 gene knockout (Muc2-/-). The following databases were used to perform the literature review: PubMed, Scopus, Web of Science, e-library, Cyberleninka, Science Direct, and Google Academy. Statistical processing of experimental data was carried out using GraphPadPrism, Statistica program package. The work was carried out at the Higher School of Biotechnology and Food Production of SPbPU and at the Department of Biochemistry, and Department ofÂ  Molecular Microbiology of the Federal State Budgetary Scientific Institution "Institute of Experimental Medicine". The main factors of pathogenesis of inflammatory bowel diseases were investigated on in vitro and in vivo models.Â  Increased permeability and development of an inflammatory response in in vitro models of IBD, and signs of inflammation in an in vivo model were observed. The considered models of IBD reflect the pathogenesis of the disease and can be used for further study of the biological effect of drugs.