title: Basement membrane and cellular migration: role of Gelatinases (MMP-2, MMP-9) on proteolysis of type IV collagen
title: Ruolo delle Gelatinasi (MMP-2, MMP-9) nel processamento del collagene IV, componente fondamentale della membrana basale cellulare
titleType: AlternativeTitle
Creators: name: MONACO, SUSANNA
Publisher: name: Università degli Studi di Roma "Tor Vergata"
Year: 2007
Subjects: subject: matrixmetalloproteinases; basement membrane; collagen IV; angiogenesis; extracellular matrix
subject: matrixmetalloproteinases
subject: basement membrane
subject: collagen IV
subject: angiogenesis
subject: extracellular matrix

description: 20. ciclo
description: I) Basement membranes: role in tumor angiogenesis - 1) basement membrane structure and assembly - a) basement membrane glycoproteins - a1) collagens - a2) other glycoproteins - 2) Basement membrane assembly - 3) Angiogenesis and vascular basement membrane - 4) Regulation of angiogenesis by MMPs . - II) Matrix Metalloproteinases (Matrixin): Structure and Function - 1) Metzincin superfamily - 2) Structural complexity of MMPs - a) the catalytic domain and the active site - b) the hemopexin-like domain - c) the hinge region - d) the fibronectin-like-domain - 3) Activation of proMMPs - a) Intracellular activation - b) stepwise activation mechanism - c) cell surface activation of proMMP2 - 4) Inhibition of MMP activity - a) Endogenous MMP inhibitors - b) Exogenous MMP inhibitors . - III) Gelatinases and type IV of collagen degradation - 1) Type IV of collagen structure - 2) Gelatinases cleavage site on type IV of collagen degradation. - Aim of the study - Results : I) Degradation of type IV of collagen from human placenta by MMP2 . - II) Degradation of type IV of collagen from human placenta by cdMMP-2 . - III) Degradation of native collagen type IV from murine (EHS) sarcoma by whole MMP-2 . - IV) Role of MMP2 on the migration of neutrophils across a type IV of collagen coating membrane . - V) Role of the fibronectin like domain of MMP-2 (CBD) on the processing of type IV of collagen from human placenta by whole MMP-2 . - VI) Degradation of type IV of collagen from human placenta by MMP-9 . - VII) Degradation of native collagen type IV from murine EHS sarcoma by wholeMMP-9 . - VIII) Cntibution of the interaction of fibronectin-like domain of MMP2 (rCBD) to the processing of collagen type IV from human placenta by MMP9 . - IX) Contribution of the interaction of the fibronectin like domain of MMP-2 (CBD) to the processing of native collagen type IV from murine EHS sarcoma by MMP9 . - X) Synergistic effect between MMP-2 and MMP-9 during neutrophil cells migration across a coating membrane of collagen type IV - Discussion Conclusion . Matherials and Methods . I) Materials . II) Methods . 1) Enzymatic activation . 2) Activity assay . 3) Kinetics of digestion of collagen type IV from human placenta and of native collagen type IV from murine EHS sarcoma by whole MMP2 and cdMMP2 . 4) Human neutrophils isolation . 5) chemotaxis assay . 6) Role of the fibronectin-like domain of MMP2 (rCBD) on the processing of collagen type IV from human placenta by whole MMP2 . 7) Kinetic analysis . 8) kinetics of digestion of collagen type IV from human placenta and of native collagen IV from murine EHS sarcoma by MMP9 . 9) Contribution of the fibronectin-like domain of MMP2 (CBD) on the processing of collagen type IV from human placenta by MMP9. 10) Contribution of the fibronectin-like domain of MMP2 (rCBD) on the processing of native collagen type IV from murine EHS sarcoma by MMP9 . 11) Kinetic analysis - Literature cited - Curriculum vitae - Articles