cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Homologous Recombination Repair Factors Rad51 and BRCA1 Are Necessary for Productive Replication of Human Papillomavirus 31
Creators: name: Moody, Cary A.
name: Johnson, Bryan A.
name: Anacker, Daniel C.
name: Gautam, Dipendra
name: Chappell, William H.
name: Ok, Suzan T.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2016

description: ABSTRACT High-risk human papillomavirus 31 (HPV31)-positive cells exhibit constitutive activation of the ATM-dependent DNA damage response (DDR), which is necessary for productive viral replication. In response to DNA double-strand breaks (DSBs), ATM activation leads to DNA repair through homologous recombination (HR), which requires the principal recombinase protein Rad51, as well as BRCA1. Previous studies from our lab demonstrated that Rad51 and BRCA1 are expressed at high levels in HPV31-positive cells and localize to sites of viral replication. These results suggest that HPV may utilize ATM activity to increase HR activity as a means to facilitate viral replication. In this study, we demonstrate that high-risk HPV E7 expression alone is sufficient for the increase in Rad51 and BRCA1 protein levels. We have found that this increase occurs, at least in part, at the level of transcription. Studies analyzing protein stability indicate that HPV may also protect Rad51 and BRCA1 from turnover, contributing to the overall increase in cellular levels. We also demonstrate that Rad51 is bound to HPV31 genomes, with binding increasing per viral genome upon productive replication. We have found that depletion of Rad51 and BRCA1, as well as inhibition of Rad51's recombinase activity, abrogates productive viral replication upon differentiation. Overall, these results indicate that Rad51 and BRCA1 are required for the process of HPV31 genome amplification and suggest that productive replication occurs in a manner dependent upon recombination. IMPORTANCE Productive replication of HPV31 requires activation of an ATM-dependent DNA damage response, though how ATM activity contributes to replication is unclear. Rad51 and BRCA1 play essential roles in repair of double-strand breaks, as well as the restart of stalled replication forks through homologous recombination (HR). Given that ATM activity is required to initiate HR repair, coupled with the requirement of Rad51 and BRCA1 for productive viral replication, our findings suggest that HPV may utilize ATM activity to ensure localization of recombination factors to productively replicating viral genomes. The finding that E7 increases the levels of Rad51 and BRCA1 suggests that E7 contributes to productive replication by providing DNA repair factors required for viral DNA synthesis. Our studies not only imply a role for recombination in the regulation of productive HPV replication but provide further insight into how HPV manipulates the DDR to facilitate the productive phase of the viral life cycle.
THE ROLE OF ICT IN AGRICULTURE IN INDIA: A REVIEW
Creators: name: Dr. Sandip Manoharrao Khanzode
Publisher: name: Zenodo
Year: 2022
Subjects: Information & Communication Technology, Agriculture, Government Initiative.

description: Abstract: Agriculture is the broadest term by which crop plants and domestication are used to refer to the many ways in which they sustain the global human population by providing food and other products. The English word agriculture comes from the Latin agar (field) and kolo (agriculture), which, when combined, refers to Latin agriculture: farm or land cultivation. Agriculture is the practice of planting crops and livestock for the benefit of the people. In this study it is studied that how the ICT plays an important role towards the development of Indian agriculture. In study Importance & Role of ICT in Agriculture, History of Indian Agriculture, Present ICT scenario in Agriculture, most useful websites for Agriculture, Governments initiatives towards ICT applications, its benefits, and its challenges.
Human Cytomegalovirus pTRS1 and pIRS1 Antagonize Protein Kinase R To Facilitate Virus Replication
Creators: name: Ziehr, Benjamin
name: Vincent, Heather A.
name: Moorman, Nathaniel J.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2016

description: ABSTRACT Human cytomegalovirus (HCMV) counteracts host defenses that otherwise act to limit viral protein synthesis. One such defense is the antiviral kinase protein kinase R (PKR), which inactivates the eukaryotic initiation factor 2 (eIF2) translation initiation factor upon binding to viral double-stranded RNAs. Previously, the viral TRS1 and IRS1 proteins were found to antagonize the antiviral kinase PKR outside the context of HCMV infection, and the expression of either pTRS1 or pIRS1 was shown to be necessary for HCMV replication. In this study, we found that expression of either pTRS1 or pIRS1 is necessary to prevent PKR activation during HCMV infection and that antagonism of PKR is critical for efficient viral replication. Consistent with a previous study, we observed decreased overall levels of protein synthesis, reduced viral protein expression, and diminished virus replication in the absence of both pTRS1 and pIRS1. In addition, both PKR and eIF2α were phosphorylated during infection when pTRS1 and pIRS1 were absent. We also found that expression of pTRS1 was both necessary and sufficient to prevent stress granule formation in response to eIF2α phosphorylation. Depletion of PKR prevented eIF2α phosphorylation, rescued HCMV replication and protein synthesis, and reversed the accumulation of stress granules in infected cells. Infection with an HCMV mutant lacking the pTRS1 PKR binding domain resulted in PKR activation, suggesting that pTRS1 inhibits PKR through a direct interaction. Together our results show that antagonism of PKR by HCMV pTRS1 and pIRS1 is critical for viral protein expression and efficient HCMV replication. IMPORTANCE To successfully replicate, viruses must counteract host defenses that limit viral protein synthesis. We have identified inhibition of the antiviral kinase PKR by the viral proteins TRS1 and IRS1 and shown that this is a critical step in HCMV replication. Our results suggest that inhibiting pTRS1 and pIRS1 function or restoring PKR activity during infection may be a successful strategy to limit HCMV disease.
Trilling Whistling — Molahlehi Matima
Creators: name: Matima, Molahlehi
name: Bruinders, Sylvia
name: Weinberg, Paul
Publisher: name: University of Cape Town
Year: 2023
Subjects: classificationCode: 360306
subject: Musicology and ethnomusicology
classificationCode: 360304
subject: Music performance
classificationCode: 451901
subject: Global Indigenous studies culture, language and history

description: This forms part of the Lesotho Music and Instrument Research Project. This is Molahlehi Matima illustrating his trademark trilling whistling, which is part of his performance on the lesiba. Filmed by Paul Weinberg in 2020.
Question Related Phenomena in Ihanzu
Creators: name: Tiemann, Elisabeth
Publisher: name: Zenodo
Year: 2022
Subjects: Languages of Tanzania
Tanzanian Rift Valley
Ihanzu
Takama
Bantu
Questions

description: A discussion of question related phenomena in Ihanzu, based on data collected with Nicolas Nalingigwa Gideon during the course "Linguistic Field Methods".
description: Note: This talk has not gone through a process of peer review, and findings should therefore be treated as preliminary and subject to change. Acknowledgement and citation: Tiemann, Elisabeth. 2022. Question Related Phenomena in Ihanzu. Talk given at the Ihanzu Symposium, Bielefeld University, Germany. 29/09/2022.
PARSEC evolutionary tracks of massive stars up to $350 M_\odot$ at metallicities 0.0001$\leq Z \leq$0.04
Creators: name: Chen, Yang
name: Bressan, Alessandro
name: Girardi, Léo
name: Marigo, Paola
name: Kong, Xu
name: Lanza, Antonio
Publisher: name: arXiv
Year: 2015
Subjects: subject: Solar and Stellar Astrophysics (astro-ph.SR)
subject: FOS: Physical sciences

description: We complement the PARSEC data base of stellar evolutionary tracks with new models of massive stars, from the pre-main sequence phase to the central carbon ignition. We consider a broad range of metallicities, 0.0001$\leq Z \leq$0.04 and initial masses up to $M_{\rm ini}=350\,M_\odot$. The main difference with respect to our previous models of massive stars is the adoption of a recent formalism accounting for the mass-loss enhancement when the ratio of the stellar to the Eddington luminosity, $Γ_e$, approaches unity. With this new formalism, the models are able to reproduce the Humphreys-Davidson limit observed in the Galactic and Large Magellanic Cloud colour-magnitude diagrams, without an ad hoc mass-loss enhancement. We also follow the predictions of recent wind models indicating that the metallicity dependence of the mass-loss rates becomes shallower when $Γ_e$ approaches unity. We thus find that the more massive stars may suffer from substantial mass-loss even at low metallicity. We also predict that the Humphreys-Davidson limit should become brighter at decreasing metallicity. We supplement the evolutionary tracks with new tables of theoretical bolometric corrections, useful to compare tracks and isochrones with the observations. For this purpose, we homogenize existing stellar atmosphere libraries of hot and cool stars (PoWR, ATLAS9 and Phoenix) and we add, where needed, new atmosphere models computed with WM-basic. The mass, age and metallicity grids are fully adequate to perform detailed investigations of the properties of very young stellar systems, both in local and distant galaxies. The new tracks supersede the previous old Padova models of massive stars.
description: Published on MNRAS. 14 pages, 8 figures
Exploring the entrepreneurial antecedents of absorptive capacity and Its impact on open, radical and incremental innovation
Creators: name: Salehi, Nader
name: Omar, Rozyta
name: Ismail, Kmariah
Publisher: name: figshare
Year: 2023
Subjects: classificationCode: 350307
subject: Technology management
classificationCode: 350704
subject: Entrepreneurship
classificationCode: 350705
subject: Innovation management
classificationCode: 350716
subject: Small business organisation and management
classificationCode: 350718
subject: Strategy

description: A critical aspect of Absorptive Capacity (AC) is the successful process of absorbing and applying new external knowledge, which drives enterprise to develop and has a continuous flow of innovation. The capability to identify the value of knowledge, acquire, assimilate, transform, and exploit knowledge is called AC. AC is dynamic capability that allows the enterprise deal with knowledge from the external environment. This capability enables enterprises to internalize knowledge and innovation to commercial ends. In the past decades, there have been studies about organizational characteristics, which may have positive or negative effects on the capability of AC, but insights about the development of AC in perspective of entrepreneurship are still limited. According to studies in entrepreneurship, in this research explore the influence of four entrepreneurial aspects on AC. Prior knowledge, alertness, intention, and orientation are four entrepreneurial aspects, which identify their relationships with AC in this research.
Geisteswissenschaftliches Forschungsdatenmanagement im Verbund - Das Data Center for the Humanities (DCH) in kollaborativen Netzwerken
Creators: name: Helling, Patrick
name: Schildkamp, Philip
name: Blumtritt, Jonathan
name: Mathiak, Brigitte
Publisher: name: Zenodo
Year: 2019
Subjects: subject: RDM
subject: Research Data Management
subject: Humanities
subject: FOS: Humanities
subject: Digital Humanities

description: Forschungsdatenmanagement innerhalb der Geisteswissenschaften zeichnet sich durch äußerst heterogene Bedarfsstrukturen aus. Zur umfassenden Abdeckung dieser Bedarfsstrukturen arbeitet das Data Center for the Humanities (DCH) in lokalen, regionalen, nationalen und internationalen Verbünden und Netzwerken. (Präsentation auf der 19. DINI-Jahrestagnung 2018)
Human Cytomegalovirus Stimulates the Synthesis of Select Akt-Dependent Antiapoptotic Proteins during Viral Entry To Promote Survival of Infected Monocytes
Creators: name: Cojohari, Olesea
name: Chan, Gary C.
name: Peppenelli, Megan A.
name: Moorman, Nathaniel J.
name: Arend, Kyle C.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2016

description: ABSTRACT Primary peripheral blood monocytes are responsible for the hematogenous dissemination of human cytomegalovirus (HCMV) following a primary infection. To facilitate viral spread, we have previously shown HCMV to extend the short 48-h life span of monocytes. Mechanistically, HCMV upregulated two specific cellular antiapoptotic proteins, myeloid leukemia sequence 1 (Mcl-1) and heat shock protein 27 (HSP27), to block the two proteolytic cleavages necessary for the formation of fully active caspase 3 and the subsequent initiation of apoptosis. We now show that HCMV more robustly upregulated Mcl-1 than normal myeloid growth factors and that Mcl-1 was the only myeloid survival factor to rapidly induce HSP27 prior to the 48-h cell fate checkpoint. We determined that HCMV glycoproteins gB and gH signal through the cellular epidermal growth factor receptor (EGFR) and αvβ3 integrin, respectively, during viral entry in order to drive the increase of Mcl-1 and HSP27 in an Akt-dependent manner. Although Akt is known to regulate protein stability and transcription, we found that gB- and gH-initiated signaling preferentially and cooperatively stimulated the synthesis of Mcl-1 and HSP27 through mTOR-mediated translation. Overall, these data suggest that the unique signaling network generated during the viral entry process stimulates the upregulation of select antiapoptotic proteins allowing for the differentiation of short-lived monocytes into long-lived macrophages, a key step in the viral dissemination strategy. IMPORTANCE Human cytomegalovirus (HCMV) infection is endemic within the human population. Although primary infection is generally asymptomatic in immunocompetent individuals, HCMV is a significant cause of morbidity and mortality in the immunocompromised. The multiorgan inflammatory diseases associated with symptomatic HCMV infection are a direct consequence of the monocyte-mediated systemic spread of the virus. In order for peripheral blood monocytes to facilitate viral dissemination, HCMV subverts the short 48-h life span of monocytes by inducing the expression of cellular antiapoptotic proteins Mcl-1 and HSP27. Here, we demonstrate that the rapid and simultaneous upregulation of Mcl-1 and HSP27 is a distinctive feature of HCMV-induced monocyte survival. Moreover, we decipher the signaling pathways activated during viral entry needed for the robust synthesis of Mcl-1 and HSP27. Identifying the virus-specific mechanisms used to upregulate select cellular factors required for the survival of HCMV-infected monocytes is important to the development of new classes of anti-HCMV drugs.
THE ROLE OF ICT IN DEVELOPMENT OF BANKING SECTOR OF INDIA: A REVIEW
Creators: name: Prof. Kishor N. Tayade
Publisher: name: Zenodo
Year: 2022
Subjects: Information & Communication Technology, Banking Services, Evolution of Banking Services.

description: Abstract: А bаnk is а business. In аny саse, similаr tо сertаin оrgаnizаtiоns, bаnks dоn't сreаte items оr соnсentrаte nоrmаl аssets frоm the eаrth. Bаnks sell mоnetаry аdministrаtiоns, fоr exаmрle, vehiсle аdvаnсes, hоme lоаn аdvаnсes, business аdvаnсes, finаnсiаl reсоrds, сhаrge саrd аdministrаtiоns, stоre аuthentiсаtiоns аnd individuаl retirement ассоunts. Bаnking аssumes аn essentiаl раrt in giving finаnсing tо bоrrоwers useful sрeсulаtiоn орen dооrs, whiсh is сritiсаl tо guаrаntee thаt the mоnetаry frаmewоrk аnd eсоnоmy run аs exрeсted аnd рrоfiсiently. Beсаuse оf the аssоrtment оf bаnks thаt exist tоdаy, it wоuld be trоublesоme, оr if nоthing else irksоme, tо mаke а meаning оf bаnking thаt аlludes tо the vаriоus exerсises оf а wide rаnge оf bаnks. In this study it studied that History of Bank, Evolution of Banking Services, Meaning & Tools of Information & Communication Technology (ICT), Benefits of ICT in Banking Services and Government initiatives towards application of ICT in Bank.
Additional file 1 of Procalcitonin in early allograft dysfunction after orthotopic liver transplantation: a retrospective single centre study
Creators: name: Frick, Katja
name: Beller, Elisabeth A.
name: Kalisvaart, Marit
name: Dutkowski, Philipp
name: Schüpbach, Reto A.
name: Klinzing, Stephanie
Publisher: name: figshare
Year: 2022
Subjects: subject: Space Science
subject: 29999 Physical Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Biotechnology
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: Cancer
subject: Science Policy
subject: Hematology

description: Additional file 1: Table S1. Baseline characteristics of IRI versus non-IRI patients. Table S2. Infections and immunosuppression of IRI versus non-IRI patients. Table S3. Baseline characteristics of IRI with PCT >15 mcg/l and IRI with PCT <15 mcg/l. Table S4. Infections and immunosuppression in IRI with PCT >15mcg/l and IRI with PCT <15 mcg/l. Table S5. Missing procalcitonin data on the basis of EAD and IRI. Table S6. Median PCT in EAD versus non-EAD during the first postoperative week. Table S7. Median PCT according to DCD and DBD in EAD and IRI. Table S8. Kidney function and renal replacement therapy in EAD versus non-EAD. Table S9. Kidney function and renal replacement therapy in IRI versus non-IRI. Table S10. Kidney function and renal replacement therapy in IRI with PCT >15 mcg/l and IRI with PCT <15 mcg/l.
Several types of types in programming languages
Creators: name: Martini, Simone
Publisher: name: arXiv
Year: 2015
Subjects: subject: Programming Languages (cs.PL)
subject: FOS: Computer and information sciences
subject: D.3.3; K.2

description: Types are an important part of any modern programming language, but we often forget that the concept of type we understand nowadays is not the same it was perceived in the sixties. Moreover, we conflate the concept of "type" in programming languages with the concept of the same name in mathematical logic, an identification that is only the result of the convergence of two different paths, which started apart with different aims. The paper will present several remarks (some historical, some of more conceptual character) on the subject, as a basis for a further investigation. The thesis we will argue is that there are three different characters at play in programming languages, all of them now called types: the technical concept used in language design to guide implementation; the general abstraction mechanism used as a modelling tool; the classifying tool inherited from mathematical logic. We will suggest three possible dates ad quem for their presence in the programming language literature, suggesting that the emergence of the concept of type in computer science is relatively independent from the logical tradition, until the Curry-Howard isomorphism will make an explicit bridge between them.
description: History and Philosophy of Computing, HAPOC 2015. To appear in LNCS
Interferon-Regulatory Factor 5-Dependent Signaling Restricts Orthobunyavirus Dissemination to the Central Nervous System
Creators: name: Lucas, Tiffany
name: Diamond, Michael S.
name: Proenca-Modena, Jose Luiz
name: Hyde, Jennifer L.
name: Gorman, Matthew J.
name: Sesti-Costa, Renata
name: Richner, Justin M.
name: Pinto, Amelia K.
name: Lazear, Helen M.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2016

description: ABSTRACT Interferon (IFN)-regulatory factor 5 (IRF-5) is a transcription factor that induces inflammatory responses after engagement and signaling by pattern recognition receptors. To define the role of IRF-5 during bunyavirus infection, we evaluated Oropouche virus (OROV) and La Crosse virus (LACV) pathogenesis and immune responses in primary cells and in mice with gene deletions in Irf3 , Irf5 , and Irf7 or in Irf5 alone. Deletion of Irf3 , Irf5 , and Irf7 together resulted in uncontrolled viral replication in the liver and spleen, hypercytokinemia, extensive liver injury, and an early-death phenotype. Remarkably, deletion of Irf5 alone resulted in meningoencephalitis and death on a more protracted timeline, 1 to 2 weeks after initial OROV or LACV infection. The clinical signs in OROV-infected Irf5 −/− mice were associated with abundant viral antigen and terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL)-positive cells in several regions of the brain. Circulating dendritic cell (DC) subsets in Irf5 −/− mice had higher levels of OROV RNA in vivo yet produced lower levels of type I IFN than wild-type (WT) cells. This result was supported by data obtained in vitro , since a deficiency of IRF-5 resulted in enhanced OROV infection and diminished type I IFN production in bone marrow-derived DCs. Collectively, these results indicate a key role for IRF-5 in modulating the host antiviral response in peripheral organs that controls bunyavirus neuroinvasion in mice. IMPORTANCE Oropouche virus (OROV) and La Crosse virus (LACV) are orthobunyaviruses that are transmitted by insects and cause meningitis and encephalitis in subsets of individuals in the Americas. Recently, we demonstrated that components of the type I interferon (IFN) induction pathway, particularly the regulatory transcription factors IRF-3 and IRF-7, have key protective roles during OROV infection. However, the lethality in Irf3 −/− Irf7 −/− (DKO) mice infected with OROV was not as rapid or complete as observed in Ifnar −/− mice, indicating that other transcriptional factors associated with an IFN response contribute to antiviral immunity against OROV. Here, we evaluated bunyavirus replication, tissue tropism, and cytokine production in primary cells and mice lacking IRF-5. We demonstrate an important role for IRF-5 in preventing neuroinvasion and the ensuing encephalitis caused by OROV and LACV.
Superfluid response of two-dimensional parahydrogen clusters in confinement
Creators: name: Idowu, Saheed
name: Boninsegni, Massimo
Publisher: name: arXiv
Year: 2015
Subjects: subject: Mesoscale and Nanoscale Physics (cond-mat.mes-hall)
subject: Statistical Mechanics (cond-mat.stat-mech)
subject: FOS: Physical sciences

description: We study by computer simulations the effect of confinement on the superfluid properties of small two-dimensional (2D) parahydrogen clusters. For clusters of fewer than twenty molecules, the superfluid response in the low temperature limit is found to remain comparable in magnitude to that of free clusters, within a rather wide range of depth and size of the confining well. The resilience of the superfluid response is attributable to the "supersolid" character of these clusters. We investigate the possibility of establishing a bulk 2D superfluid "cluster crystal" phase of \paraH2, in which a global superfluid response would arise from tunnelling of molecules across adjacent unit cells. The computed energetics suggests that for clusters of about ten molecules, such a phase may be thermodynamically stable against the formation of the equilibrium insulating crystal, for values of the cluster crystal lattice constant possibly allowing tunnelling across adjacent unit cells
A NEW NORMAL PHASE OF DIGITALIZATION FOR FINANCIAL SERVICES: A STUDY OF CUSTOMER'S PERCEPTION TOWARDS CRYPTOCURRENCIES
Creators: name: Dr. Ruma Pal1    Dr. Pulkit Trivedi2
Publisher: name: Zenodo
Year: 2022
Subjects: Cryptocurrency, Ethereum (ETH), Digitalization, Bitcoin (BTC), Binance Coin (BNB), Tether (USDT)

description: Abstract Crypto currencies are a sort of digital currency that is comprised of and designed to function as a string of binary data. The term "crypt currency" refers to any digital or virtual currency that uses cryptography for transaction security. When it comes to cryptocurrency, a decentralised system keeps track of transactions and issues new units rather than a centralised issuing or regulatory authority. The use of centralised institutions such as banks to verify financial transactions is rendered obsolete by the cryptocurrency's use of a decentralised digital currency known as cryptocurrency. Users located in any part of the world are able to send and receive monetary transactions thanks to the decentralised nature of the network. Transactions made with a cryptocurrency only exist as digital records in a public ledger. The transaction is recorded in a public archive when buying or selling cryptocurrency. Cryptocurrency is kept in digital wallets. The key impartial of this study is to inspect how people view cryptocurrencies and whether or not they view them as a consecration or an expletive. So for conducting the research project sample size of 200 respondents was selected. The vital information regarding the research was collected through a questionnaire. SPSS has been used to run some analyses, giving us a better grasp of the data.
Lekope Piece — Matlali Kheoana
Creators: name: Bruinders, Sylvia
name: Weinberg, Paul
Publisher: name: University of Cape Town
Year: 2023
Subjects: classificationCode: 360306
subject: Musicology and ethnomusicology
classificationCode: 360304
subject: Music performance
classificationCode: 451901
subject: Global Indigenous studies culture, language and history

description: In January 2020, traditional musicians from Lesotho met in Morija, inspired by the sculptures of Samuele Makoanyane, who was commissioned by the ethnomusicologist Percival Kirby in 1934. This forms part of the Lesotho Music and Instrument Research Project. Matlali Kheoana performs on the lekope. Filmed by Paul Weinberg in 2020.
Additional file 1 of Extra benefit of microalgae in raw piggery wastewater treatment: pathogen reduction
Creators: name: Lee, Sang-Ah
name: Kim, Minsik
name: Kim, Hee-Sik
name: Ahn, Chi-Yong
Publisher: name: figshare
Year: 2022
Subjects: subject: Microbiology
subject: FOS: Biological sciences

description: Additional file 1: Figure S1. Results of next-generation sequencing in family level in both control and microalgae treated group. Data are summarized for 30 most abundant genera and noted as legends. Figure S2. Results of next-generation sequencing in order level in both control and microalgae treated group. Data are summarized for 30 most abundant genera and noted as legends. Figure S3. Results of next-generation sequencing in class level in both control and microalgae treated group. Data are summarized for 30 most abundant genera and noted as legends. Figure S4. Phylogenetic tree of isolated Oligella sp. and ASVs assigned as Oligella genus constructed by the neighbor-joining method. Figure S5. Scatter plot, trend line, 95% confidence band, and 95% prediction band between algal growth and relative abundance of ASV1 (Oligella). Figure S6. Scatter plot between relative abundances of algae-positive bacteria and ASV1 (Oligella). Table S1. Compositions and physicochemical properties of the raw wastewater, effluent of control (aerobic digestion), and microalgal treatment, respectively. The effluents were analyzed by using culture broth after 96 hours of treatment. Table S2. Summary of non-metric multidimensional scaling (NMDS) results with PERMANOVA test indicating significance level of *: P < 0.05 and **: P < 0.01. Table S3. Inhibitory zone diameters in range and mean with standard deviation (SD) values induced by various microorganisms to Oligella sp. The tests were quadruplicated. Table S4. Correlation, p-value and Benjamini-Hochberg adjusted p-value of Pearson correlation between Oligella and algal growth, Brevundimonas, Sphingopyxis, and Stenotrophomonas.
Oedera dieterlenii N. G. Bergh. 2018, comb. nov.
Creators: name: Bergh, Nicola G.
name: Bentley, Joanne
name: Verboom, George Anthony
Publisher: name: Zenodo
Year: 2018
Subjects: Biodiversity
Taxonomy
Plantae
Tracheophyta
Magnoliopsida
Asterales
Asteraceae
Oedera
Oedera dieterlenii

description: 3. Oedera dieterlenii (Phillips) N.G.Bergh, comb. nov.
 Basionym: Nestlera dieterlenii Phillips (1917: 344). Relhania dieterlenii (Phillips) Bremer (1976a: 29).—Type: LESOTHO, stony and sunny spot on slopes of Roma Kloof, 1914, Jacotter 111, Herb. Dieterlen No. 1065 (PRE lectotype! [designated by Bremer 1976a]; P!, Z, isolectotypes).
 Section IV. Oedera sect. Stipitatae N.G.Bergh, sect. nov.
 — Comborhiza Anderberg & Bremer (1991: 1070), syn. nov. — Type species: Comborhiza virgata (N.E.Br.) Anderberg & K.Bremer — Oreoleysera Bremer (1978b: 450), syn. nov. — Type species: Oreoleysera montana (Bolus) K.Bremer
 Type species: Oedera virgata (N.E.Brown) N.G.Bergh. (Comborhiza virgata (N.E.Brown) Anderberg & K.Bremer)
 Description: woody shrublets or subshrubs, two of the species resprouting from a subterranean rootstock. Leaves crowded on branches, alternate or fasciculate on lateral brachyblasts, entire, somewhat fleshy, with distinct midrib, both surfaces densely invested with glandular trichomes on elongate slender stalks. Capitula broad, solitary, sessile and axillary or on long peduncles or pedunculoid stems, sometimes flowering branches highly ramified to produce a racemose or cymose synflorescence. Receptacle epaleate, sometimes also shortly squamose. Ray florets 10–25; disc florets 25–125, perfect or possibly functionally male in one species (Oe. longipes). Pappus of short more-or-less connate scales only (Oe. virgata), or of scales and a few outer additional barbellate bristles on the disc florets. Cypselas glabrous to pilose, more densely pilose on ray cypselas.
 Cytology: 2n = 14 (Oedera virgata; Bremer, 1976a).
description: Published as part of Bergh, Nicola G., Bentley, Joanne & Verboom, George Anthony, 2018, Classification of the Relhania generic group (Asteraceae, Gnaphalieae) revisited using molecular phylogenetic analysis, pp. 101-132 in Phytotaxa 344 (2) on page 118, DOI: 10.11646/phytotaxa.344.2.1, http://zenodo.org/record/13719571
Streaming Multicast Video over Software-Defined Networks
Creators: name: Noghani, Kyoomars Alizadeh
name: Sunay, M. Oguz
Publisher: name: arXiv
Year: 2015
Subjects: subject: Networking and Internet Architecture (cs.NI)
subject: FOS: Computer and information sciences

description: Many of the video streaming applications in today's Internet involve the distribution of content from a CDN source to a large population of interested clients. However, widespread support of IP multicast is unavailable due to technical and economical reasons, leaving the floor to application layer multicast which introduces excessive delays for the clients and increased traffic load for the network. This paper is concerned with the introduction of an SDN-based framework that allows the network controller to not only deploy IP multicast between a source and subscribers, but also control, via a simple northbound interface, the distributed set of sources where multiple- description coded (MDC) video content is available. We observe that for medium to heavy network loads, relative to the state-of-the-art, the SDN-based streaming multicast video framework increases the PSNR of the received video significantly, from a level that is practically unwatchable to one that has good quality.
description: 6 pages, 5 figures, 1 table, First Workshop on Software-Defined Internets of the Future - WSDIF 2014. Proceedings of the 11th IEEE International Conference on Mobile Ad hoc and Sensor Systems (MASS) 2014 - Philadelphia, Pennsylvania - October 28 - 30, 2014
Kaposi's Sarcoma-Associated Herpesvirus Latency Locus Compensates for Interleukin-6 in Initial B Cell Activation
Creators: name: Dittmer, Dirk P.
name: Eason, Anthony
name: Kim, Yongbaek
name: Kang, Sun Ah
name: Sin, Sang-Hoon
name: Tan, Kelly
name: An, Hyowon
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2016

description: Interleukin 6 (IL-6) is considered a proliferation and survival factor for B cells. To assess the role of IL-6 in Kaposi sarcoma-associated herpesvirus (KSHV) latency, KSHV latency locus-transgenic mice (referred to as latency mice) lacking IL-6 were evaluated. IL-6 −/− latency mice had the same phenotypes as the latency mice, i.e., increased frequency of marginal zone B cells, hyperplasia, and hyperglobulinemia, indicating that the KSHV latency locus, which includes all viral microRNAs (miRNAs), can compensate for lack of IL-6 in premalignant B cell activation.
Oedera montana N. G. Bergh 2018, comb. nov.
Creators: name: Bergh, Nicola G.
name: Bentley, Joanne
name: Verboom, George Anthony
Publisher: name: Zenodo
Year: 2018
Subjects: Biodiversity
Taxonomy
Plantae
Tracheophyta
Magnoliopsida
Asterales
Asteraceae
Oedera
Oedera montana

description: 4. Oedera montana (Bolus) N.G.Bergh, comb. nov.
 Basionym: Leysera montana Bolus (1905: 138). Oreoleysera montana (Bolus) Bremer (1978b: 450).—Type: SOUTH AFRICA,”In monte Matroosberg ditione Worscester”, 6500 ped [ft], January 1897, A.Bolus 6367 (BOL, holotype!).
description: Published as part of Bergh, Nicola G., Bentley, Joanne & Verboom, George Anthony, 2018, Classification of the Relhania generic group (Asteraceae, Gnaphalieae) revisited using molecular phylogenetic analysis, pp. 101-132 in Phytotaxa 344 (2) on page 119, DOI: 10.11646/phytotaxa.344.2.1, http://zenodo.org/record/13719571
Multiple Transcripts Encode Full-Length Human Cytomegalovirus IE1 and IE2 Proteins during Lytic Infection
Creators: name: Moorman, Nathaniel J.
name: Arend, Kyle C.
name: Vincent, Heather A.
name: Ziehr, Benjamin
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2016

description: ABSTRACT Expression of the human cytomegalovirus (HCMV) IE1 and IE2 proteins is critical for the establishment of lytic infection and reactivation from viral latency. Defining the mechanisms controlling IE1 and IE2 expression is therefore important for understanding how HCMV regulates its replicative cycle. Here we identify several novel transcripts encoding full-length IE1 and IE2 proteins during HCMV lytic replication. Two of the alternative major immediate early (MIE) transcripts initiate in the first intron, intron A, of the previously defined MIE transcript, while others extend the 5′ untranslated region. Each of the MIE transcripts associates with polyribosomes in infected cells and therefore contributes to IE1 and IE2 protein levels. Surprisingly, deletion of the core promoter region of the major immediate early promoter (MIEP) from a plasmid containing the MIE genomic locus did not completely abrogate IE1 and IE2 expression. Instead, deletion of the MIEP core promoter resulted in increased expression of alternative MIE transcripts, suggesting that the MIEP suppresses the activity of the alternative MIE promoters. While the canonical MIE mRNA was the most abundant transcript at immediate early times, the novel MIE transcripts accumulated to levels equivalent to that of the known MIE transcript later in infection. Using two HCMV recombinants, we found that sequences in intron A of the previously defined MIE transcript are required for efficient IE1 and IE2 expression and viral replication. Together, our results identify new regulatory sequences controlling IE1 and IE2 expression and suggest that multiple transcription units act in concert to regulate IE1 and IE2 expression during lytic infection. IMPORTANCE The HCMV IE1 and IE2 proteins are critical regulators of HCMV replication, both during primary infection and reactivation from viral latency. This study expands our understanding of the sequences controlling IE1 and IE2 expression by defining novel transcriptional units controlling the expression of full-length IE1 and IE2 proteins. Our results suggest that alternative promoters may allow for IE1 and IE2 expression when MIEP activity is limiting, as occurs in latently infected cells.
Additional file 1 of Complete chloroplast genome of the desert date (Balanites aegyptiaca (L.) Del. comparative analysis, and phylogenetic relationships among the members of Zygophyllaceae
Creators: name: AL-Juhani, Widad S.
name: Alharbi, Samah A.
name: Al Aboud, Nora M.
name: Aljohani, Ashwaq Y.
Publisher: name: figshare
Year: 2022
Subjects: subject: Genetics
subject: FOS: Biological sciences

description: Additional file 1.
Bekenstein-Hawking Entropy and Strange Metals
Creators: name: Sachdev, Subir
Publisher: name: arXiv
Year: 2015
Subjects: subject: High Energy Physics - Theory (hep-th)
subject: Strongly Correlated Electrons (cond-mat.str-el)
subject: General Relativity and Quantum Cosmology (gr-qc)
subject: FOS: Physical sciences

description: We examine models of fermions with infinite-range interactions which realize non-Fermi liquids with a continuously variable U(1) charge density $\mathcal{Q}$, and a non-zero entropy density $\mathcal{S}$ at vanishing temperature. Real time correlators of operators carrying U(1) charge $q$ at a low temperature $T$ are characterized by a $\mathcal{Q}$-dependent frequency $ω_{\mathcal{S}} = (q \, T/\hbar) (\partial \mathcal{S}/\partial{\mathcal{Q}})$ which determines a spectral asymmetry. We show that the correlators match precisely with those of the AdS$_2$ horizons of extremal charged black holes. On the black hole side, the matching employs $\mathcal{S}$ as the Bekenstein-Hawking entropy density, and the laws of black hole thermodynamics which relate $(\partial{\mathcal{S}}/\partial{\mathcal{Q}})/(2 π)$ to the electric field strength in AdS$_2$. The fermion model entropy is computed using the microscopic degrees of freedom of a UV complete theory without supersymmetry.
description: 23 pages, 2 figures; (v2, v3, v4) added refs and clarifications
Recovery of West Nile Virus Envelope Protein Domain III Chimeras with Altered Antigenicity and Mouse Virulence
Creators: name: Bente, Dennis A.
name: McAuley, Alexander J.
name: Beasley, David W.C.
name: Plante, Jessica A.
name: Huang, Claire Y.-H.
name: Torres, Maricela
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2016

description: ABSTRACT Flaviviruses are positive-sense, single-stranded RNA viruses responsible for millions of human infections annually. The envelope (E) protein of flaviviruses comprises three structural domains, of which domain III (EIII) represents a discrete subunit. The EIII gene sequence typically encodes epitopes recognized by virus-specific, potently neutralizing antibodies, and EIII is believed to play a major role in receptor binding. In order to assess potential interactions between EIII and the remainder of the E protein and to assess the effects of EIII sequence substitutions on the antigenicity, growth, and virulence of a representative flavivirus, chimeric viruses were generated using the West Nile virus (WNV) infectious clone, into which EIIIs from nine flaviviruses with various levels of genetic diversity from WNV were substituted. Of the constructs tested, chimeras containing EIIIs from Koutango virus (KOUV), Japanese encephalitis virus (JEV), St. Louis encephalitis virus (SLEV), and Bagaza virus (BAGV) were successfully recovered. Characterization of the chimeras in vitro and in vivo revealed differences in growth and virulence between the viruses, with in vivo pathogenesis often not being correlated with in vitro growth. Taken together, the data demonstrate that substitutions of EIII can allow the generation of viable chimeric viruses with significantly altered antigenicity and virulence. IMPORTANCE The envelope (E) glycoprotein is the major protein present on the surface of flavivirus virions and is responsible for mediating virus binding and entry into target cells. Several viable West Nile virus (WNV) variants with chimeric E proteins in which the putative receptor-binding domain (EIII) sequences of other mosquito-borne flaviviruses were substituted in place of the WNV EIII were recovered, although the substitution of several more divergent EIII sequences was not tolerated. The differences in virulence and tissue tropism observed with the chimeric viruses indicate a significant role for this sequence in determining the pathogenesis of the virus within the mammalian host. Our studies demonstrate that these chimeras are viable and suggest that such recombinant viruses may be useful for investigation of domain-specific antibody responses and the more extensive definition of the contributions of EIII to the tropism and pathogenesis of WNV or other flaviviruses.
Additional file 1 of An exploratory study of associations between the ICD-11 personality disorder model and eating pathology
Creators: name: Stricker, Johannes
name: Barthels, Friederike
name: Müller, Romina
name: Pietrowsky, Reinhard
Publisher: name: figshare
Year: 2022
Subjects: subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Neuroscience
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Cancer
subject: Science Policy
subject: 111714 Mental Health
subject: FOS: Health sciences

description: Additional file 1. Supplementary statistical information.
Oedera longipes N. G. Bergh 2018, comb. nov.
Creators: name: Bergh, Nicola G.
name: Bentley, Joanne
name: Verboom, George Anthony
Publisher: name: Zenodo
Year: 2018
Subjects: Biodiversity
Taxonomy
Plantae
Tracheophyta
Magnoliopsida
Asterales
Asteraceae
Oedera
Oedera longipes

description: 5. Oedera longipes (K.Bremer) N.G.Bergh, comb. nov.
 Basionym: Leysera longipes Bremer (1978a: 381). Comborhiza longipes (K.Bremer) Anderberg & Bremer (1991: 1070).—Type: SOUTH AFRICA, Cape Province, Tulbagh Division, path up to the Sneeugat, c. 2,000 ft, January 1913, Esterhuysen 30022 (BOL, holotype!; S, iso).
description: Published as part of Bergh, Nicola G., Bentley, Joanne & Verboom, George Anthony, 2018, Classification of the Relhania generic group (Asteraceae, Gnaphalieae) revisited using molecular phylogenetic analysis, pp. 101-132 in Phytotaxa 344 (2) on page 119, DOI: 10.11646/phytotaxa.344.2.1, http://zenodo.org/record/13719571
The K1 Protein of Kaposi's Sarcoma-Associated Herpesvirus Augments Viral Lytic Replication
Creators: name: Brulois, Kevin F.
name: Damania, Blossom
name: Zhang, Zhigang
name: Dittmer, Dirk P.
name: Chen, Wuguo
name: Sanders, Marcia K.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2016

description: ABSTRACT The K1 gene product of Kaposi's sarcoma-associated herpesvirus (KSHV) is encoded by the first open reading frame (ORF) of the viral genome. To investigate the role of the K1 gene during the KSHV life cycle, we constructed a set of recombinant viruses that contained either wild-type (WT) K1, a deleted K1 ORF (KSHVΔK1), stop codons within the K1 ORF (KSHV-K1 5×STOP ), or a revertant K1 virus (KSHV-K1 REV ). We report that the recombinant viruses KSHVΔK1 and KSHV-K1 5×STOP displayed significantly reduced lytic replication compared to WT KSHV and KSHV-K1 REV upon reactivation from latency. Additionally, cells infected with the recombinant viruses KSHVΔK1 and KSHV-K1 5×STOP also yielded smaller amounts of infectious progeny upon reactivation than did WT KSHV- and KSHV-K1 REV -infected cells. Upon reactivation from latency, WT KSHV- and KSHV-K1 REV -infected cells displayed activated Akt kinase, as evidenced by its phosphorylation, while cells infected with viruses deleted for K1 showed reduced phosphorylation and activation of Akt kinase. Overall, our results suggest that K1 plays an important role during the KSHV life cycle. IMPORTANCE Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiological agent of three human malignancies, and KSHV K1 is a signaling protein that has been shown to be involved in cellular transformation and to activate the phosphatidylinositol 3-kinase (PI3K)/Akt/mTOR pathway. In order to investigate the role of the K1 protein in the life cycle of KSHV, we constructed recombinant viruses that were deficient for K1. We found that K1 deletion viruses displayed reduced lytic replication compared to the WT virus and also yielded smaller numbers of infectious progeny. We report that K1 plays an important role in the life cycle of KSHV.
Additional file 1 of Lactic acid from vaginal microbiota enhances cervicovaginal epithelial barrier integrity by promoting tight junction protein expression
Creators: name: Delgado-Diaz, David Jose
name: Jesaveluk, Brianna
name: Hayward, Joshua A.
name: Tyssen, David
name: Alisoltani, Arghavan
name: Potgieter, Matthys
name: Bell, Liam
name: Ross, Elizabeth
name: Iranzadeh, Arash
name: Allali, Imane
name: Dabee, Smritee
name: Barnabas, Shaun
name: Gamieldien, Hoyam
name: Blackburn, Jonathan M.
name: Mulder, Nicola
name: Smith, Steven B.
name: Edwards, Vonetta L.
name: Burgener, Adam D.
name: Bekker, Linda-Gail
name: Ravel, Jacques
name: Passmore, Jo-Ann S.
name: Masson, Lindi
name: Hearps, Anna C.
name: Tachedjian, Gilda
Publisher: name: figshare
Year: 2022
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Physiology
subject: Biotechnology
subject: Immunology
subject: FOS: Clinical medicine
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics
subject: Cancer
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Additional file 1: Figure S1. Association between cervicovaginal pH and relative abundance of bacterial L- and D-lactate dehydrogenase [LDH; Log2 intensity based absolute quantification (iBAQ) values]. Figure S2. Multidimensional scaling (MDS) plot of genes differentially expressed by ectocervical epithelial cells treated with 0.3% L-lactic acid (L-LA) pH 3.9 or D-lactic acid (D-LA) pH 3.9 (yellow and blue respectively), HCl pH 3.9 (green) or untreated cells (red). Figure S3. Primary cervicovaginal epithelial cells were apically treated with 0.3% L-lactic acid pH (L-LA, pH 3.9), or low pH media alone (HCl, pH 3.9) for 1 h and gene expression of tight junction factors claudin-1 (CLDN1) and claudin-4 (CLDN4) were assessed 4 h post-treatment by qRT-PCR.
Time-reversible dynamics in a system of two coupled active rotators
Creators: name: Burylko, Oleksandr
name: Wolfrum, Matthias
name: Yanchuk, Serhiy
name: Kurths, Jürgen
Publisher: name: Weierstrass Institute
Year: 2023
Subjects: subject: Coupled rotators
subject: time-reversibility
subject: 34C15
subject: 34C14
subject: 34C23

description: We study two coupled active rotators with Kuramoto-type coupling and focus our attention to specific transitional regimes where the coupling is neither attractive nor repulsive. We show that certain such situations at the edge of synchronization can be characterized by the existence of a time-reversal symmetry of the system. We identify two different cases with such a time-reversal symmetry. The first case is characterized by a non-reciprocal attractive/repulsive coupling. The second case is a reciprocal coupling exactly at the edge between attraction and repulsion. We give a detailed description of possible different types of dynamics and bifurcations for both cases. In particular, we show how the time-reversible coupling can induce both oscillation death and oscillation birth to the active rotators. Moreover, we analyse the coexistence of conservative and dissipative regions in phase space, which is a typical feature of systems with a time-reversal symmetry. We show also, how perturbations breaking the time-reversal symmetry and destroying the conservative regions can lead to complicated types of dissipative dynamics such as the emergence of long-period cycles showing a bursting-like behavior.