title: ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð° Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ ÐºÐ¾Ð¼Ð¿ÐµÐ½ÑÐ°ÑÐ¾ÑÐ½ÑÑ Ð¼Ð¸Ð ÐÐ Ð¿ÑÐ¾ÑÐ¸Ð² Ð²Ð¸ÑÑÑÐ° Ð³ÑÐ¸Ð¿Ð¿Ð° Ð in vitro
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Investigation of the action possibilities of compensatory siRNAs against influenza A virus in vitro
Creators: name: ÐÑÑÐ°ÑÐµÐ²Ð°, ÐÐ»ÐµÐºÑÐ°Ð½Ð´ÑÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: Ð²Ð¸ÑÑÑ Ð³ÑÐ¸Ð¿Ð¿Ð° Ð
Ð ÐÐ-Ð¸Ð½ÑÐµÑÑÐµÑÐµÐ½ÑÐ¸Ñ
Ð¼Ð¸Ð ÐÐ
influenza Ð virus
RNA-interference
siRNA

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ in vitro Ð¿ÑÐ¾ÑÐ¸Ð²Ð¾Ð²Ð¸ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð° ÑÐ¸Ð½ÑÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð¼Ð¸Ð ÐÐ, Ð°Ð½Ð°Ð»Ð¾Ð³Ð¾Ð² ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð¼Ð¸Ð ÐÐ, Ð°ÑÑÐ¾ÑÐ¸Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ Ñ Ð¿ÑÐ¾ÑÐµÑÑÐ¾Ð¼ Ð²Ð¸ÑÑÑÐ½Ð¾Ð¹ ÑÐµÐ¿ÑÐ¾Ð´ÑÐºÑÐ¸Ð¸ Ð² ÐºÐ»ÐµÑÐºÐµ. ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: 1. ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÑ Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ ÑÑÐµÐ´ÑÑÐ²Ð° Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ Ð´Ð¾ÑÑÐ°Ð²ÐºÐ¸ ÑÐ¸Ð½ÑÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ ÐºÐ¾Ð¼Ð¿ÐµÐ½ÑÐ°ÑÐ¾ÑÐ½ÑÑ Ð¼Ð¸Ð ÐÐ Ð´Ð²Ðµ ÑÐ¸ÑÑÐµÐ¼Ñ Ð³Ð¸Ð±ÑÐ¸Ð´Ð½ÑÑ Ð¼Ð¸ÐºÑÐ¾Ð½Ð¾ÑÐ¸ÑÐµÐ»ÐµÐ¹: ÑÑÐ±- Ð¸ Ð¼Ð¸ÐºÑÐ¾Ð½Ð½ÑÑ ÐºÐ°Ð¿ÑÑÐ». 2. ÐÑÐµÐ½Ð¸ÑÑ Ð¿ÑÐ¾ÑÐ¸Ð²Ð¾Ð²Ð¸ÑÑÑÐ½ÑÐ¹ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð» in vitro Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ° Ð¼Ð¸Ð ÐÐ Ð² ÑÐ¾ÑÑÐ°Ð²Ðµ Ð¼Ð¸ÐºÑÐ¾Ð½Ð¾ÑÐ¸ÑÐµÐ»ÐµÐ¹ Ð½Ð° ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ ÐºÑÐ»ÑÑÑÑÐµ Ð549 Ð² Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ð¸ ÑÑÐ°Ð¼Ð¼Ð° A/PR/8/34. Ð Ð°Ð±Ð¾ÑÐ° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ Â«ÐÐ°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ ÑÐ¸ÑÑÐµÐ¼Ð½Ð¾Ð¹ Ð²Ð¸ÑÑÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸Â» Ð¤ÐÐÐ£ Â«ÐÐÐ Ð³ÑÐ¸Ð¿Ð¿Ð° Ð¸Ð¼ÐµÐ½Ð¸ Ð.Ð. Ð¡Ð¼Ð¾ÑÐ¾Ð´Ð¸Ð½ÑÐµÐ²Ð°Â», Ð³Ð´Ðµ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»Ð°ÑÑ Ð½ÐµÐ¿Ð¾ÑÑÐµÐ´ÑÑÐ²ÐµÐ½Ð½Ð°Ñ Ð½Ð°ÑÑÐ½Ð¾-Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÑÐºÐ°Ñ ÑÐ°Ð±Ð¾ÑÐ°: Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ðµ Ð¾ÑÐµÐ½ÐºÐ¸ ÑÑÐ¾Ð²Ð½Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð°-Ð¼Ð¸ÑÐµÐ½Ð¸ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÐ¸ Ð¼Ð¸Ð ÐÐ miR-16, Ð¿Ð¾ÑÑÑÐ¾ÐµÐ½Ð¸Ðµ ÐºÐ°Ð»Ð¸Ð±ÑÐ¾Ð²Ð¾ÑÐ½Ð¾Ð¹ ÐºÑÐ¸Ð²Ð¾Ð¹ Ð´Ð»Ñ Ð¾ÑÐµÐ½ÐºÐ¸ ÑÑÐ¾Ð²Ð½Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð¼Ð¸Ð ÐÐ, Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ðµ Ð´Ð²ÑÑ ÑÐ¸Ð¿Ð¾Ð² Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ Ð´Ð¾ÑÑÐ°Ð²ÐºÐ¸ Ð² Ð²Ð¸Ð´Ðµ ÑÑÐ±- Ð¸ Ð¼Ð¸ÐºÑÐ¾Ð½Ð½ÑÑ Ð¿Ð¾Ð»Ð¸Ð°ÑÐ³Ð¸Ð½Ð¸Ð½Ð¾Ð²ÑÑ ÐºÐ°Ð¿ÑÑÐ» Ñ ÑÐ¾ÑÐºÐ¸ Ð·ÑÐµÐ½Ð¸Ñ Ð·Ð°Ð³ÑÑÐ·Ð¾ÑÐ½Ð¾Ð¹ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÐ¸, ÑÐ¾ÐºÑÐ¸ÑÐ½Ð¾ÑÑÐ¸ Ð¸ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð´Ð¾ÑÑÐ°Ð²ÐºÐ¸. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð±ÑÐ»Ð° Ð¿Ð¾Ð´ÑÐ²ÐµÑÐ¶Ð´ÐµÐ½Ð° Ð¿ÑÐ¾ÑÐ¸Ð²Ð¾Ð²Ð¸ÑÑÑÐ½Ð°Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ° Ð¼Ð¸Ð ÐÐ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ ÑÐ¸ÑÑÐµÐ¼Ñ Ð´Ð¾ÑÑÐ°Ð²ÐºÐ¸ Ð² Ð²Ð¸Ð´Ðµ Ð¼Ð¸ÐºÑÐ¾ÐºÐ°Ð¿ÑÑÐ», Ð¾Ð´Ð½Ð°ÐºÐ¾ Ð±ÑÐ»Ð° Ð¾ÑÐ¿Ð¾ÑÐµÐ½Ð° ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÑÐ¸ÑÑÐµÐ¼Ñ ÑÑÐ±Ð¼Ð¸ÐºÑÐ¾ÐºÐ°Ð¿ÑÑÐ».
description: This work is devoted to the in vitro study of the antiviral potential of synthetic siRNAs, analogues of cellular microRNAs associated with the process of viral reproduction in the cell. Tasks that were solved during the study: 1. Investigate two systems of hybrid microcarriers: sub- and micron capsules as a means of intracellular delivery of synthetic compensatory siRNAs. 2. Evaluate the antiviral potential in vitro of the siRNA preparation as a part of microcarriers on cell culture A549 in relation to strain A / PR / 8/34. The work was carried out on the basis of the âLaboratory of System Virologyâ âSmorodintsev Research Institute of Influenzaâ, where research was directly carried out: assessing the level of expression of the target gene of the miRNA-16 sequence, making a calibration curve to assess the level of siRNA expression, comparing two types of intracellular delivery in the form of sub- and micron polyarginine capsules in terms of loading ability, toxicity and delivery efficiency. As a result, the antiviral activity of the siRNA preparation based on the microcapsule delivery system was confirmed, however, the effectiveness of the submicrocapsule system was challenged.