cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Relationships between social climate and indoor environmental quality and frequently reported health symptoms among teachers and staff in a suburban school district
Creators: name: Martenies, Sheena E.
name: Schill, Jennifer
name: Klimm, Matthew
name: Cross, Jennifer E.
name: Oliver, Shannon
name: Magzamen, Sheryl
Publisher: name: Taylor & Francis
Year: 2022
Subjects: subject: Medicine
subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Science Policy
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Public school teachers represent one of the largest occupational groups in the United States and are vulnerable to job stress and burnout. School social and physical environments may be adversely impacting the health of teachers and other staff, though few studies have explored these relationships. We partnered with a suburban school district in Colorado to assess the association between school environmental quality, social climate, and staff member health. We modeled the number of self-reported frequent health symptoms (experienced at least once a week) using generalized linear models. School-level predictors of interest included: overall social climate scores (unitless), building operations report card (ORC) scores (unitless), and indoor air quality (IAQ) scores (unitless). In total, we had data from 134 staff members from 11 schools in the district. A majority (62%) of our participants were teachers, who reported a greater number of frequent (i.e., at least once a week) health symptoms (mean = 3.2 symptoms experienced at least once per week) compared to staff in other roles (mean = 2.3 symptoms per week). We found that a one standard-deviation (10.5) increase in the overall social climate score was associated with a 0.77-fold (95% CI: 0.60–0.99) change in the number of frequent health symptoms reported. However, this association was attenuated among teachers compared to other staff members. Our results suggested effect modification by social climate on the relationship between IAQ and health, albeit with some uncertainty. For participants with a school climate score below the mean, a one standard-deviation (10.5) increase in IAQ score was associated with a 0.49-fold (95% CI: 0.35–0.70) change in the number of frequently reported symptoms. Overall, our study suggests school climate may be associated with self-reported health symptoms, but that the benefits of improved school climates may not be as strong for teachers compared to other staff. Future work should assess perceived climate at the individual level to assess how staff roles impact how school environments are associated with health outcomes.
(1711) Benjamin Willans
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Relationships between social climate and indoor environmental quality and frequently reported health symptoms among teachers and staff in a suburban school district
Creators: name: Martenies, Sheena E.
name: Schill, Jennifer
name: Klimm, Matthew
name: Cross, Jennifer E.
name: Oliver, Shannon
name: Magzamen, Sheryl
Publisher: name: Taylor & Francis
Year: 2022
Subjects: subject: Medicine
subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Science Policy
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Public school teachers represent one of the largest occupational groups in the United States and are vulnerable to job stress and burn out. School social and physical environments may be adversely impacting the health of teachers and other staff, though few studies have explored these relationships. We partnered with a suburban school district in Colorado to assess the association between school environmental quality, social climate, and staff member health. We modeled the number of self-reported frequent health symptoms (experienced at least once a week) using generalized linear models. School-level predictors of interest included: overall social climate scores (unitless), building operations report card (ORC) scores (unitless), and indoor air quality (IAQ) scores (unitless). In total, we had data from 134 staff members from 11 schools in the district. A majority (62%) of our participants were teachers, who reported a greater number of frequent (i.e., at least once a week) health symptoms (mean =3.2 symptoms experienced at least once per week) compared to staff in other roles (mean =2.3 symptoms per week). We found that a one standard deviation (10.5) increase in the overall social climate score was associated with a 0.77-fold (95%CI: 0.60 – 0.99) change in the number of frequent health symptoms reported. However, this association was attenuated among teachers compared to other staff members. Our results suggested effect modification by social climate on the relationship between IAQ and health, albeit with some uncertainty. For participants with a school climate score below the mean, a one standard deviation (10.5) increase in IAQ score was associated with a 0.49-fold (95% CI: 0.35 – 0.70) change in the number of frequently reported symptoms. Overall, our study suggests school climate may be associated with self-reported health symptoms, but that the benefits of improved school climates may not be as strong for teachers compared to other staff. Future work should assess perceived climate at the individual level to assess how staff roles impact how school environments are associated with health outcomes.
Auscultation des zones d'interface des remblais des barrages en terre avec les ouvrages en béton
Creators: name: SMAIL, Nadia
name: ROUISSAT, Bouchrit
Publisher: name: Academic Journal of Civil Engineering
Year: 2021

description: La surveillance des barrages, par l'auscultation, vise principalement à suivre et à interpréter les évolutions des mécanismes de dégradations ou de vieillissement en relation avec les critères sécuritaires du barrage, en particulier, sa résistance aux déformations et aux infiltrations d’eau. Pour les barrages en terre, les zones d’interface du remblai avec les structures en béton représentent des points vulnérables à ces deux phénomènes. L’auscultation de ces zones par des cellules de pressions interstitielles et totales constitue un support pour l’analyse du comportement du barrage. Dans ce contexte, on s'intéresse au barrage de Boughrara, situé au nord ouest de l'Algérie. Il s'agit d'un barrage zoné à noyau central en argile, d'une hauteur de 60 m. On traitera de l'analyse de l'évolution des pressions totales dans les interfaces des remblais avec les ouvrages en béton armé, en l'occurrence, la galerie de dérivation provisoire et la zone de l'évacuateur de crue du barrage. Cette analyse de l'évolution des pressions totales, mesurées pendant 14 années, est conjuguée à celle des valeurs mesurées des pressions interstitielles et des charges des remblais y afférentes. Ce couplage de contraintes a permis de réaliser un diagnostic de l'ouvrage et un jugement sur la validation de son comportement général.
description: Academic Journal of Civil Engineering, Vol 38 No 2 (2021): Special Issue - Diagnobéton 2021
Supplementary Table 2 from α-Amylase immobilization on amidoximated acrylic microfibres activated by cyanuric chloride.
Creators: name: Yaaser Q. Almulaiky
name: Aqlan, Faisal M.
name: Musab Aldhahri
name: Baeshen, Mohammed
name: Khan, Tariq Jamal
name: Khan, Khalid A.
name: Afifi, Mohamed
name: AL-Farga, Ammar
name: Mohiuddin Khan Warsi
name: Alkaled, Mohammed
name: Alayafi, Aisha A. M.
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: Biochemistry
subject: 90301 Biomaterials
subject: FOS: Medical engineering

description: metal ion effects on soluble and immobilized α-amylase.
Regulators of G Protein Signaling and Transient Activation of Signaling: EXPERIMENTAL AND COMPUTATIONAL ANALYSIS REVEALS NEGATIVE AND POSITIVE FEEDBACK CONTROLS ON G PROTEIN ACTIVITY
Creators: name: Yildirim, Necmettin
name: Elston, Timothy C.
name: Dohlman, Henrik G.
name: Hao, Nan
name: Wang, Yuqi
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2003

description: Cellular responses to hormones and neurotransmitters are necessarily transient. The mating pheromone signal in yeast is typical. Signal initiation requires cell surface receptors, a G protein heterotrimer, and down-stream effectors. Signal inactivation requires Sst2, a regulator of G protein signaling (RGS) protein that accelerates GTPase activity. We conducted a quantitative analysis of RGS and G protein expression and devised computational models that describe their activity in vivo. These results indicated that pheromone-dependent transcriptional induction of the RGS protein constitutes a negative feedback loop that leads to desensitization. Modeling also suggested the presence of a positive feedback loop leading to resensitization of the pathway. In confirmation of the model, we found that the RGS protein is ubiquitinated and degraded in response to pheromone stimulation. We identified and quantitated these positive and negative feedback loops, which account for the transient response to external signals observed in vivo.
(1713) John Buckingham Pope
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
A field-portable colorimetric method for the measurement of peracetic acid vapors: A comparison of glass and plastic impingers
Creators: name: Stastny, Angela L.
name: Doepke, Amos
name: Streicher, Robert P.
Publisher: name: Taylor & Francis
Year: 2022
Subjects: subject: Biophysics
subject: Biochemistry
subject: Space Science
subject: 29999 Physical Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Genetics
subject: Molecular Biology
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences

description: A method for measuring peracetic acid vapors in air using impinger sampling and field-portable colorimetric analysis is presented. The capture efficiency of aqueous media in glass and plastic impingers was evaluated when used for peracetic acid vapor sampling. Measurement of peracetic acid was done using an N,N-diethyl-p-phenylenediamine colorimetric method with a field portable spectrometer. The linearity of the N,N-diethyl-p-phenylenediamine method was determined for peracetic acid both in-solution and captured from vapor phase using glass or plastic impingers. The Limits of Detection for the glass and plastic impingers were 0.24 mg/m3 and 0.28 mg/m3, respectively, for a 15 L air sample. The Limits of Quantitation were 0.79 mg/m3 and 0.92 mg/m3 for the glass and plastic impingers, respectively. Both metrics were below the American Conference of Governmental Industrial Hygienists Threshold Limit Value Short-Term Exposure Limit of 1.24 mg/m3 (0.4 ppmv) during a 15-minute period. This impinger sampling method presented herein allows for an easy to use and rapid in-field measurement that can be used for evaluating occupational exposure to peracetic acid.
The source of widespread 3-$μ$m absorption in Jupiter's clouds: Constraints from 2000 Cassini VIMS observations
Creators: name: Sromovsky, Lawrence
name: Fry, Patrick
Publisher: name: arXiv
Year: 2015
Subjects: subject: Earth and Planetary Astrophysics (astro-ph.EP)
subject: FOS: Physical sciences

description: The Cassini flyby of Jupiter in 2000 provided spatially resolved spectra of Jupiter's atmosphere using the Visual and Infrared Mapping Spectrometer (VIMS). These spectra contain a strong absorption at wavelengths from about 2.9 $μ$m to 3.1 $μ$m, previously noticed in a 3-$μ$m spectrum obtained by the Infrared Space Observatory (ISO) in 1996. While Brooke et al. (1998, Icarus 136, 1-13) were able to fit the ISO spectrum very well using ammonia ice as the sole source of particulate absorption, Sromovsky and Fry (2010, Icarus 210, 211-229), using significantly revised NH$_3$ gas absorption models, showed that ammonium hydrosulfide (NH$_4$SH) provided a better fit to the ISO spectrum than NH$_3$ , but that the best fit was obtained when both NH$_3$ and NH$_4$SH were present. Although the large FOV of the ISO instrument precluded identification of the spatial distribution of these two components, the VIMS spectra at low and intermediate phase angles show that 3-$μ$m absorption is present in zones and belts, in every region investigated, and both low- and high-opacity samples are best fit with a combination of NH$_4$SH and NH$_3$ particles at all locations. The best fits are obtained with a layer of small ammonia-coated particles ($r\sim0.3$ $μ$m) overlying but often close to an optically thicker but still modest layer of much larger NH$_4$SH particles ($r\sim 10$ $μ$m), with a deeper optically thicker layer, which might also be composed of NH$_4$SH. Although these fits put NH$_3$ ice at pressures less than 500 mb, this is not inconsistent with the lack of prominent NH$_3$ features in Jupiter's longwave spectrum because the reflectivity of the core particles strongly suppresses the NH$_3$ absorption features, at both near-IR and thermal wavelengths.
description: 33 pages, 28 figures, 4 tables
First Survey on the presence of anisakid parasites in farmed European sea bass and gilthead sea bream produced and marketed in Sicily
Creators: name: Cammilleri, Gaetano
name: Costa, Antonella
name: Graci, Stefania
name: Buscemi, Maria Drussilla
name: Collura Rosaria
name: Giangrosso, Giuseppe
Publisher: name: INFN Open Access Repository
Year: 2018

description: The Scientific Opinion of EFSA (2010) outlines as the only fish free of health risks related to Anisakidae parasites the farmed salmon, if reared in floating cages or on-shore cages and fed by feed with no live parasites. Otherwise, the food operator shall verify, by means of procedures approved by the competent authority, that the fishery products do not represent a health risk regarding the presence of live parasites. This opinion was confirmed by several studies conducted on farmed salmon in Norway (Angot and Brasseur, 1993, Aquaculture, 118: 339-344; Lunestad, 2003, J Food Prot, 66:122-124). The absence of anisakid parasites in farmed fish has also been found for other marine fish species such as European sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus aurata) (Penalver et al., 2010 J Food Prot, 73:1332-1334). Currently there are low evidences on the prevalence of anisakid infestation in aquaculture fish produced and marketed in Italy. In this work, a sampling plan was carried out aimed at collecting different fish samples marketed in Sicily, to verify the presence and prevalence of anisakid parasites infestation. A total of 143 samples of sea bass and 110 samples of sea bream from Sicilian and Greek farms were examined: all the samples were of commercial size (over 200 g). The specimens were taken and stored refrigerated then transferred to the C.Re.N.A laboratories, where visual inspection of viscera and muscle was carried out. The negative samples were subjected to chloro-peptic digestion. The larvae found were subjected to morphological identification, through optical microscopy and molecular analysis by PCRRFLP method. The survey revealed the presence of two parasites belonging to the Anisakidae family, found inside the coelomatic cavity, only in a single sample of European sea bass from a single farm located in Greece, revealing a prevalence of infestation of 1.7%. The larvae were morphologically identified as belonging to the morphotype I of the genus Anisakis. Molecular investigations confirmed the larvae as Anisakis pegreffii species. No larvae were found in the samples of gilthead sea bream examined. The present work represents the first report on the presence of anisakid parasites in European sea bass. Our findings in farmed fish can be traced back to the aquaculture policies; however the prevalence of infestation in these productive realities remains very low. Furthermore, the results of this study suggest further investigations in order to have a comprehensive risk picture.
Supplementary Table 4 from α-Amylase immobilization on amidoximated acrylic microfibres activated by cyanuric chloride
Creators: name: Yaaser Q. Almulaiky
name: Aqlan, Faisal M.
name: Musab Aldhahri
name: Baeshen, Mohammed
name: Khan, Tariq Jamal
name: Khan, Khalid A.
name: Afifi, Mohamed
name: AL-Farga, Ammar
name: Mohiuddin Khan Warsi
name: Alkaled, Mohammed
name: Alayafi, Aisha A. M.
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: Biochemistry
subject: 90301 Biomaterials
subject: FOS: Medical engineering

description: Inhibitor effects on soluble and immobilized α-amylase.
Requirement of Cys 399 for Processing of the Human Ecto-ATPase (NTPDase2) and Its Implications for Determination of the Activities of Splice Variants of the Enzyme
Creators: name: Kreda, Silvia
name: Boyer, José L.
name: Mateo, Jesús
name: Henry, Christopher E.
name: Harden, T. Kendall
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2003

description: Ecto-ATPase (CD39L1) corresponds to the type 2 enzyme of the ecto-nucleoside triphosphate diphosphohydrolase family (E-NTPDase). We have isolated from human ECV304 cells three cDNAs with high homology with members of the E-NTPDase family that encode predicted proteins of 495, 472, and 450 amino acids. Sequencing of a genomic DNA clone confirmed that these three sequences correspond to splice variants of the human ecto-ATPase (NTPDase2 alpha,-2 beta, and -2 gamma). Although all three enzyme forms were expressed heterologously to similar levels in Chinese hamster ovary cells clone K-1 (CHO-K1) cells, only the 495-amino acid protein (NTPDase2 alpha exhibited ecto-ATPase activity. Immunolocalization studies demonstrated that NTPDase2 alpha is fully processed and trafficked to the plasma membrane, whereas the NTPDase2 beta and -2 gamma splice variants were retained in not fully glycosylated forms in the endoplasmic reticulum. The potential roles of two highly conserved residues, Cys399 and Asn443, in the activity and cellular trafficking of the ecto-ATPase were examined. Mutation of Cys399, which is absent in NTPDase2 beta and -2 gamma, produced a protein completely devoid of nucleotidase activity, while mutation of Asn443 to Asp resulted in substantial loss of activity. Neither the Cys399 nor Asn443 mutants were fully glycosylated, and both were retained in the endoplasmic reticulum. These results indicate that the lack of ecto-nucleotidase activity exhibited by NTPDase2 beta and -2 gamma and the C399S mutant, as well as the large reduction of activity in the N443D mutant are due to alterations in the folding/maturation of these proteins.
ERTAKLARNI INGLIZ TILIGA INTEGRATSIYA QILISHDA AXBOROT TEXNOLOGIK VOSITALARIDAN FOYDALANISH USULLARI
Creators: name: Tashpulatova, Madina
Publisher: name: Zenodo
Year: 2025

description: O'zbek xalq ertaklari nafaqat milliy madaniyatimizning ajralmas qismi, balki bolalarning axloqiy va estetik tarbiyasida muhim rol o'ynaydi. Ushbu ertaklar o'quvchilarga ijobiy qadriyatlarni singdirish, til boyligini oshirish va ijodiy fikrlashni rivojlantirishda samarali vosita hisoblanadi. Zamonaviy ta'lim tizimida ingliz tilini o'rgatishda interfaol va innovatsion metodlarni qo'llash talab etiladi. Ertaklarni ingliz tiliga integratsiya qilish, o'quvchilarning til o'rganish jarayonini qiziqarli va samarali qilishga yordam beradi. Axborot-kommunikatsiya texnologiyalarining (AKT) rivojlanishi bilan birga, bu jarayonni yanada interaktiv va samarali tashkil etish imkoniyatlari paydo bo'ldi. Ertaklarni ingliz tiliga integratsiya qilishda AKT vositalaridan foydalanish, o'quvchilarning til ko'nikmalarini rivojlantirishda muhim ahamiyatga ega.
Algorithms and complexity for Turaev-Viro invariants
Creators: name: Burton, Benjamin A.
name: Maria, Clément
name: Spreer, Jonathan
Publisher: name: arXiv
Year: 2015
Subjects: subject: Geometric Topology (math.GT)
subject: Computational Complexity (cs.CC)
subject: Data Structures and Algorithms (cs.DS)
subject: Mathematical Software (cs.MS)
subject: FOS: Mathematics
subject: FOS: Computer and information sciences
subject: F.2.2; G.2.1; G.4
subject: 57M27, 57Q15, 68Q17

description: The Turaev-Viro invariants are a powerful family of topological invariants for distinguishing between different 3-manifolds. They are invaluable for mathematical software, but current algorithms to compute them require exponential time. The invariants are parameterised by an integer $r \geq 3$. We resolve the question of complexity for $r=3$ and $r=4$, giving simple proofs that computing Turaev-Viro invariants for $r=3$ is polynomial time, but for $r=4$ is \#P-hard. Moreover, we give an explicit fixed-parameter tractable algorithm for arbitrary $r$, and show through concrete implementation and experimentation that this algorithm is practical---and indeed preferable---to the prior state of the art for real computation.
description: 17 pages, 5 figures
Continuous NHANES survey data for environmental ambient and occupational hazard identification – Feasibility and preliminary findings for osteoporosis and kidney disease
Creators: name: Park, Robert M.
name: An, Yu
Publisher: name: Taylor & Francis
Year: 2022
Subjects: subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Physiology
subject: Pharmacology
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: Immunology
subject: FOS: Clinical medicine
subject: Marine Biology
subject: Science Policy

description: The Continuous NHANES Survey provides detailed health and environmental chemical burden information on the U.S. population. As of 2012, there were data for 71,000 participants. Based on single biomarker determinations cumulative burdens were estimated. Because age distributions would differ comparing ambient environmental and occupational exposures, a procedure to distinguish ambient from likely occupational exposures was applied. Associations are reported for osteoporosis and kidney disease-related outcomes with cadmium, lead and other metals. Cumulative cadmium burden (from blood cadmium, ambient and occupational) was a strong predictor of bone fracture risk and ambient tungsten also had a positive association. Cumulative lead (ambient and occupational) had a negative (“protective”) association with fractures as did mercury (occupational). Bone mineral density was statistically significantly and similarly predicted by metal exposures. Kidney disease was significantly associated with cumulative lead burdens from both the estimated ambient and occupational sources and with ambient blood cadmium but was most strongly associated with cumulative occupational uranium burden. Systolic blood pressure statistically significantly increased with cumulative ambient and occupational lead (blood) burden and with ambient cadmium and cobalt. Diastolic blood pressure was significantly associated with several cadmium and cobalt metrics along with ambient and occupational cumulative burdens for lead. For environmental substances with burden half-lives measured in years, NHANES offers opportunities for hypothesis generation and confirmation.
(1706) Elizabeth Wrigley
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Evaluation of surface disinfection methods to inactivate the beta coronavirus Murine Hepatitis Virus
Creators: name: Hardison, R. L.
name: Nelson, S. W.
name: Barriga, D.
name: Ruiz, N. Feliciano
name: Ghere, J. M.
name: Fenton, G. A.
name: Lindstrom, D. J.
name: James, R. R.
name: Stewart, M. J.
name: Lee, S. D.
name: Calfee, M. W.
name: Ryan, S. P.
name: Howard, M. W.
Publisher: name: Taylor & Francis
Year: 2022
Subjects: subject: Biochemistry
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: Inorganic Chemistry
subject: Science Policy
subject: Hematology
subject: 60506 Virology

description: The list of EPA-approved disinfectants for coronavirus features many products for use on hard, non-porous materials. There are significantly fewer products registered for use on porous materials. Further, many common, high-touch surfaces fall in between non-porous materials such as glass and porous materials such as soft fabrics. The objective of this study was to assess the efficacy of selected commercially available disinfectant products against coronaviruses on common, high-touch surfaces. Four disinfectants (Clorox Total 360, Bleach solution, Vital Oxide, and Peroxide Multi-Surface Cleaner) were evaluated against Murine Hepatitis Virus A59 (MHV) as a surrogate coronavirus for SARS-CoV-2. MHV in cell culture medium was inoculated onto four materials: stainless steel, latex-painted drywall tape, Styrene Butadiene rubber (rubber), and bus seat fabric. Immediately (T0) or 2-hr (T2) post-inoculation, disinfectants were applied by trigger-pull or electrostatic sprayer and either held for recommended contact times (Spray only) or immediately wiped (Spray and Wipe). Recovered infectious MHV was quantified by median tissue culture infectious dose assay. Bleach solution, Clorox Total 360, and Vital Oxide were all effective (>3-log10 reduction or complete kill of infectious virus) with both the Spray Only and Spray and Wipe methods on stainless steel, rubber, and painted drywall tape when used at recommended contact times at both T0 and T2 hr. Multi-Surface Cleaner unexpectedly showed limited efficacy against MHV on stainless steel within the recommended contact time; however, it showed increased (2.3 times greater efficacy) when used in the Spray and Wipe method compared to Spray Only. The only products to achieve a 3-log10 reduction on fabric were Vital Oxide and Clorox Total 360; however, the efficacy of Vital Oxide against MHV on fabric was reduced to below 3-log10 when applied by an electrostatic sprayer compared to a trigger-pull sprayer. This study highlights the importance of considering the material, product, and application method when developing a disinfection strategy for coronaviruses on high-touch surfaces.
RGS6, RGS7, RGS9, and RGS11 Stimulate GTPase Activity of G i Family G-proteins with Differential Selectivity and Maximal Activity
Creators: name: Krumins, Andrejs M.
name: Bodor, Erik T.
name: Waldo, Gary L.
name: Corbitt, James
name: Harden, T. Kendall
name: Hooks, Shelley B.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2003

description: Regulator of G-protein signaling (RGS) proteins are GTPase activating proteins (GAPs) of heterotrimeric G-proteins that alter the amplitude and kinetics of receptor-promoted signaling. In this study we defined the G-protein alpha-subunit selectivity of purified Sf9 cell-derived R7 proteins, a subfamily of RGS proteins (RGS6, -7, -9, and -11) containing a Ggamma-like (GGL) domain that mediates dimeric interaction with Gbeta(5). Gbeta(5)/R7 dimers stimulated steady state GTPase activity of Galpha-subunits of the G(i) family, but not of Galpha(q) or Galpha(11), when added to proteoliposomes containing M2 or M1 muscarinic receptor-coupled G-protein heterotrimers. Concentration effect curves of the Gbeta(5)/R7 proteins revealed differences in potencies and efficacies toward Galpha-subunits of the G(i) family. Although all four Gbeta(5)/R7 proteins exhibited similar potencies toward Galpha(o), Gbeta(5)/RGS9 and Gbeta(5)/RGS11 were more potent GAPs of Galpha(i1), Galpha(i2), and Galpha(i3) than were Gbeta(5)/RGS6 and Gbeta(5)/RGS7. The maximal GAP activity exhibited by Gbeta(5)/RGS11 was 2- to 4-fold higher than that of Gbeta(5)/RGS7 and Gbeta(5)/RGS9, with Gbeta(5)/RGS6 exhibiting an intermediate maximal GAP activity. Moreover, the less efficacious Gbeta(5)/RGS7 and Gbeta(5)/RGS9 inhibited Gbeta(5)/RGS11-stimulated GTPase activity of Galpha(o). Therefore, R7 family RGS proteins are G(i) family-selective GAPs with potentially important differences in activities.
Multidiffusion des ondes GPR dans le béton : simulation et expérimentation
Creators: name: Balayssac, Jean-Paul
Publisher: name: Academic Journal of Civil Engineering
Year: 2021

description: Cette communication présente les résultats obtenus au cours du projet ANR DCND. L’objectif est de mettre en évidence la multidiffusion des ondes GPR dans le béton et de proposer des méthodes d’exploitation de ce phénomène pour la caractérisation de l’état du matériau, comme cela est fait avec les ondes ultrasonores. Les effets de la multidiffusion sont d’abord mis en évidence par le biais de simulations numériques réalisées avec le logiciel GPRmax. Les résultats numériques révèlent bien l’apparition d’ondes incohérentes lorsque la taille des granulats augmente et que la longueur d’onde diminue et sur ces signaux la technique dite d’interférométrie de la coda (CWI) utilisée pour les ondes US permet de détecter de très faibles variations de la permittivité de la matrice cimentaire. Des essais avec un GPR classique sur des corps d’épreuve réels avec des inclusions de différentes tailles révèlent que l’interférométrie permet de suivre le séchage du béton, avec une sensibilité beaucoup plus importante que celle obtenue sur la variation d’atténuation de l’onde cohérente.
description: Academic Journal of Civil Engineering, Vol 38 No 2 (2021): Special Issue - Diagnobéton 2021
RhoA and ROCK Promote Migration by Limiting Membrane Protrusions
Creators: name: Burridge, Keith
name: Worthylake, Rebecca A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2003

description: Previously, we and others have shown that RhoA and ROCK signaling are required for negatively regulating integrin-mediated adhesion and for tail retraction of migrating leukocytes. This study continues our investigation into the molecular mechanisms underlying RhoA/ROCK-regulated integrin adhesion. We show that inhibition of ROCK up-regulates integrin-mediated adhesion, which is accompanied by both increased phosphotyrosine signaling through Pyk-2 and paxillin and inappropriate membrane protrusions. We provide evidence that inhibition of ROCK induces integrin adhesion by promoting remodeling of the actin cytoskeleton. Furthermore, we find that ROCK regulates membrane activity through a pathway involving cofilin. Inhibition of RhoA signaling allows the formation of multiple competing lamellipodia that disrupt productive migration of monocytes. Together, our results show that RhoA/ROCK signaling promotes migration by restricting integrin activity and membrane protrusions to the leading edge.
Supplementary Table 2 from α-Amylase immobilization on amidoximated acrylic microfibres activated by cyanuric chloride
Creators: name: Yaaser Q. Almulaiky
name: Aqlan, Faisal M.
name: Musab Aldhahri
name: Baeshen, Mohammed
name: Khan, Tariq Jamal
name: Khan, Khalid A.
name: Afifi, Mohamed
name: AL-Farga, Ammar
name: Mohiuddin Khan Warsi
name: Alkaled, Mohammed
name: Alayafi, Aisha A. M.
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: Biochemistry
subject: 90301 Biomaterials
subject: FOS: Medical engineering

description: substrate specificity of soluble and immobilized α-amylase.
Separate Roles for the Golgi Apparatus and Lysosomes in the Sequestration of Drugs in the Multidrug-resistant Human Leukemic Cell Line HL-60
Creators: name: Duvvuri, Muralikrishna
name: Krise, Jeffrey P.
name: Gong, Yuping
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2003

description: The sequestration of drugs away from cellular target sites into cytoplasmic organelles of multidrug-resistant (MDR) cancer cells has been recently shown to be a cause for ineffective drug therapy. This process is poorly understood despite the fact that it has been observed in a large number of MDR cancer cell lines. Analysis of drug sequestration in these cells has traditionally been done using fluorescent anthracycline antibiotics (i.e. daunorubicin, doxorubicin). This narrow selection of substrates has resulted in a limited understanding of sequestration mechanisms and the intracellular compartments that are involved. To better characterize this phenotype, we chose to examine the sequestration of molecules having different acid/base properties in the MDR HL-60 human leukemic cell line. Here we show that weakly basic drug daunorubicin is sequestered into lysosomes according to a pH partitioning type mechanism, whereas sulforhodamime 101, a zwitterionic molecule, is sequestered into the Golgi apparatus through a drug transporter-mediated process. Quantitative intracellular pH measurements reveal that the lysosome-tocytosol pH gradient is expanded in the MDR line. Moreover, the MDR cells overexpress the multidrug resistance-related protein (MRP1), which is localized to the Golgi apparatus. These results demonstrate, for the first time, that two distinct mechanisms for intracellular compartmentalization are operational in a single MDR cell line.
Serine Phosphorylation Negatively Regulates RhoA in Vivo
Creators: name: Burridge, Keith
name: Ellerbroek, Shawn M.
name: Wennerberg, Krister
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2003

description: Previous work indicates that RhoA phosphorylation on Ser188 by cAMP or cGMP-dependent kinases inhibits its activity. However, these studies lacked the possibility to directly study phosphorylated RhoA activity in vivo. Therefore, we created RhoA proteins containing phosphomimetic residues in place of the cAMP/cGMP-dependent kinase phosphorylation site. RhoA phosphorylation or phosphomimetic substitution did not affect Rho guanine nucleotide exchange factor, GTPase activating protein, or geranylgeranyl transferase activity in vitro but promoted binding to the Rho guanine-dissociation inhibitor as measured by exchange factor competition assays. The in vitro similarities between RhoA phosphomimetic proteins and phosphorylated RhoA allowed us to study function of phosphorylated RhoA in vivo. RhoA phosphomimetic proteins display depressed GTP loading when transiently expressed in NIH 3T3 cells. Stable-expressing RhoA and RhoA(S188A) clones spread significantly slower than mock-transfected or RhoA(S188E) clones. RhoA(S188A) clones were protected from the morphological effects of a cAMP agonist, whereas phosphomimetic clones exhibit stress fiber disassembly similar to control cells. Together, these data provide in vivo evidence that addition of a charged group to Ser188 upon phosphorylation negatively regulates RhoA activity and indicates that this occurs through enhanced Rho guanine-dissociation inhibitor interaction rather than direct perturbation of guanine nucleotide exchange factor, GTPase activating protein, or geranylgeranyl transferase activity.
PRIMA INDAGINE SULLA PRESENZA DI PARASSITI ANISAKIDI IN SPIGOLE E ORATE D'ALLEVAMENTO COMMERCIALIZZATE E PRODOTTE IN SICILIA
Creators: name: Cammilleri, Gaetano
name: Costa, Antonella
name: Vella, Antonio
name: Graci, Stefania
name: Buscemi
name: Collura
name: Ganci
name: Incardona
name: Iannazzo
name: Santangelo
name: Giangrosso
name: Ferrantelli
Publisher: name: INFN Open Access Repository
Year: 2017

description: Il presente studio rappresenta il primo report sulla presenza di parassiti anisakidi in orate e spigole di allevamento. Il ritrovamento di anisakidi in pesci allevati può essere ricondotto alle diverse politiche d’acquacoltura; tuttavia la prevalenza di infestazione in queste realtà produttive si rivela ancora bassissima. Non avendo informazioni dettagliate sulla natura dell’allevamento si presume che l’esemplare risultato positivo provenga da realtà di allevamento offshore, dove è più plausibile la presenza di organismi ospiti del parassita oggetto di studio. La prevalenza di infestazione da parte di parassiti anisakidi in queste realtà produttive si rivela ancora bassissima, tuttavia i risultati di questo studio suggeriscono ulteriori indagini al fine di avere un quadro del rischio esaustivo.
Structural Impact of the Leukemia Drug 1-β-d-Arabinofuranosylcytosine (Ara-C) on the Covalent Human Topoisomerase I-DNA Complex
Creators: name: Stewart, Lance
name: Chrencik, Jill E.
name: Pommier, Yves
name: Burgin, Alex B.
name: Redinbo, Matthew R.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2003

description: 1-beta-d-Arabinofuranosylcytosine (Ara-C) is a potent antineoplastic drug used in the treatment of acute leukemia. Previous biochemical studies indicated the incorporation of Ara-C into DNA reduced the catalytic activity of human topoisomerase I by decreasing the rate of single DNA strand religation by the enzyme by 2-3-fold. We present the 3.1 A crystal structure of human topoisomerase I in covalent complex with an oligonucleotide containing Ara-C at the +1 position of the non-scissile DNA strand. The structure reveals that a hydrogen bond formed between the 2'-hydroxyl of Ara-C and the O4' of the adjacent -1 base 5' to the damage site stabilizes a C3'-endo pucker in the Ara-C arabinose ring. The structural distortions at the site of damage are translated across the DNA double helix to the active site of human topoisomerase I. The free sulfhydryl at the 5'-end of the nicked DNA strand in this trapped covalent complex is shifted out of alignment with the 3'-phosphotyrosine linkage at the catalytic tyrosine 723 residue, producing a geometry not optimal for religation. The subtle structural changes caused by the presence of Ara-C in the DNA duplex may contribute to the cytotoxicity of this leukemia drug by prolonging the lifetime of the covalent human topoisomerase I-DNA complex.
Local Spectroscopic Characterization of Spin and Layer Polarization in WSe$_2$
Creators: name: Yankowitz, Matthew
name: McKenzie, Devin
name: LeRoy, Brian J.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Mesoscale and Nanoscale Physics (cond-mat.mes-hall)
subject: Materials Science (cond-mat.mtrl-sci)
subject: FOS: Physical sciences

description: We report scanning tunneling microscopy (STM) and spectroscopy (STS) measurements of monolayer and bilayer WSe$_2$. We measure a band gap of 2.21 $\pm$ 0.08 eV in monolayer WSe$_2$, which is much larger than the energy of the photoluminescence peak indicating a large excitonic binding energy. We additionally observe significant electronic scattering arising from atomic-scale defects. Using Fourier transform STS (FT-STS), we map the energy versus momentum dispersion relations for monolayer and bilayer WSe$_2$. Further, by tracking allowed and forbidden scattering channels as a function of energy we infer the spin texture of both the conduction and valence bands. We observe a large spin-splitting of the valence band due to strong spin-orbit coupling, and additionally observe spin-valley-layer coupling in the conduction band of bilayer WSe$_2$.
description: 5 pages, 4 figures
Supplementary Figures from Interactions between the sexual identity of the nervous system and the social environment mediate lifespan in Drosophila melanogaster.
Creators: name: Flintham, Ewan O.
name: Yoshida, Tomoyo
name: Smith, Sophie
name: Pavlou, Hania J.
name: Goodwin, Stephen F.
name: Carazo, Pau
name: Wigby, Stuart
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: Evolutionary Biology
subject: 60801 Animal Behaviour

description: Supplementary figures which compare the behaviour and lifespan profiles of male and female fly control strains. As outlined in the main text, different control strains were not deemed to differ in lifespan or behaviour in a significant way in either sex and were pooled during analyses in to single 'control' groups.
Développement d’un capteur de corrosion autonome RFID pour la surveillance des infrastructures
Creators: name: Bouzaffour, Karim
name: Lescop, Benoit
name: Talbot, Philippe
name: Gallée, François
name: Rioual, Stephane
Publisher: name: Academic Journal of Civil Engineering
Year: 2021

description: La corrosion des armatures est un paramètre fondamental de la durabilité des ouvrages en environnement marin. Elle conduit en effet avec la formation de produits de corrosion à l’éclatement du béton d’enrobage. L’objectif de ce travail est de produire un capteur totalement passif, de faible cout, et de faible dimension pouvant être immergé dans la couche d’enrobage et permettant le suivi de la corrosion. Il est basé sur la technologie UHF-RFID et peut donc être interrogé par des lecteurs commerciaux. Cet article détaille le principe physique du capteur.
description: Academic Journal of Civil Engineering, Vol 38 No 2 (2021): Special Issue - Diagnobéton 2021
Targeted Deletion of the Ileal Bile Acid Transporter Eliminates Enterohepatic Cycling of Bile Acids in Mice
Creators: name: Wilson, Martha
name: Kluckman, Kimberly
name: Tietjen, Mary
name: Maeda, Nobuyo
name: Craddock, Ann L.
name: Parks, John S.
name: Haywood, Jamie
name: Dawson, Paul A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2003

description: The ileal apical sodium bile acid cotransporter participates in the enterohepatic circulation of bile acids. In patients with primary bile acid malabsorption, mutations in the ileal bile acid transporter gene (Slc10a2) lead to congenital diarrhea, steatorrhea, and reduced plasma cholesterol levels. To elucidate the quantitative role of Slc10a2 in intestinal bile acid absorption, the Slc10a2 gene was disrupted by homologous recombination in mice. Animals heterozygous (Slc10a2+/-) and homozygous (Slc10a2-/-) for this mutation were physically indistinguishable from wild type mice. In the Slc10a2-/- mice, fecal bile acid excretion was elevated 10- to 20-fold and was not further increased by feeding a bile acid binding resin. Despite increased bile acid synthesis, the bile acid pool size was decreased by 80% and selectively enriched in cholic acid in the Slc10a2-/- mice. On a low fat diet, the Slc10a2-/- mice did not have steatorrhea. Fecal neutral sterol excretion was increased only 3-fold, and intestinal cholesterol absorption was reduced only 20%, indicating that the smaller cholic acid-enriched bile acid pool was sufficient to facilitate intestinal lipid absorption. Liver cholesteryl ester content was reduced by 50% in Slc10a2-/- mice, and unexpectedly plasma high density lipoprotein cholesterol levels were slightly elevated. These data indicate that Slc10a2 is essential for efficient intestinal absorption of bile acids and that alternative absorptive mechanisms are unable to compensate for loss of Slc10a2 function.
Supplementary Table 2 from α-Amylase immobilization on amidoximated acrylic microfibres activated by cyanuric chloride
Creators: name: Yaaser Q. Almulaiky
name: Aqlan, Faisal M.
name: Musab Aldhahri
name: Baeshen, Mohammed
name: Khan, Tariq Jamal
name: Khan, Khalid A.
name: Afifi, Mohamed
name: AL-Farga, Ammar
name: Mohiuddin Khan Warsi
name: Alkaled, Mohammed
name: Alayafi, Aisha A. M.
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: Biochemistry
subject: 90301 Biomaterials
subject: FOS: Medical engineering

description: metal ion effects on soluble and immobilized α-amylase.
Die Alternative für Deutschland
eine gefährliche Kraft im gesellschaftlichen Wandel
Creators: name: Schroeder, Wolfgang
name: Weßels, Bernhard
Publisher: name: Universität Kassel
Year: 2020
Subjects: subject: Alternative für Deutschland
subject: Parlamentarisierung
subject: Rechtspopulismus
subject: 300
subject: 320
subject: Alternative für Deutschland
subject: Rechtspopulismus
subject: Einflussnahme

description: Gefördert im Rahmen des Projekts DEAL
The Receptor Activator of Nuclear Factor-κB Ligand-mediated Osteoclastogenic Pathway Is Elevated in Amelogenin-null Mice
Creators: name: Hatakeyama, Yuji
name: Thyagarajan, Tamizchelvi
name: Sreenath, Taduru
name: Kulkarni, Ashok B.
name: Shum, Lillian
name: Wright, J. Tim
name: Hatakeyama, Junko
name: Gibson, Carolyn W.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2003

description: Amelogenins, major components of developing enamel, are predominantly involved in the formation of tooth enamel. Although amelogenins are also implicated in cementogenesis, their precise spatial expression pattern and molecular role are not clearly understood. Here, we report for the first time the expression of two alternate splice forms of amelogenins, M180 and the leucine-rich amelogenin peptide (LRAP), in the periodontal region of mouse tooth roots. Lack of M180 and LRAP mRNA expression correlated with cementum defects observed in the amelogenin-null mice. The cementum defects were characterized by an increased presence of multinucleated cells, osteoclasts, and cementicles. These defects were associated with an increased expression of the receptor activator of the nuclear factor-kappa B ligand (RANKL), a critical regulator of osteoclastogenesis. These findings indicate that the amelogenin splice variants, M180 and LRAP, are critical in preventing abnormal resorption of cementum.