cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Targeted disruption of hsp70.1 sensitizes to osmotic stress
Creators: name: Shim, E.-H.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2002

description: The 70 kDa heat shock protein (Hsp70) plays a critical role in cell survival and thermotolerance in response to various stress stimuli. Two nearly identical genes, hsp70.1 and hsp70.3, in response to environmental stress, rapidly induce Hsp70. However, it remains unclear whether these two genes are differentially regulated by various stresses. To address the physiological role of the hsp70.1 and hsp70.3 genes in the stress response, we generated mice that specifically lack hsp70.1. In contrast to heat shock, which rapidly induced both hsp70.1 and hsp70.3 mRNA, osmotic stress selectively induced transcription of hsp70.1. In hsp70.1-deficient embryonic fibroblasts, osmotic stress markedly reduced cell viability. Furthermore, when osmotic stress was applied in vivo, hsp70.1-deficient mice exhibited increased apoptosis in the renal medulla. Taken together, our results demonstrate that differential expression of hsp70 genes contributes to the stress response and that the hsp70.1 gene plays a critical role in osmotolerance.
Unionicola (Unionicola) crassipes Muller 1776
Creators: name: Pešić, Vladimir
name: Chatterjee, Tapas
name: Bordoloi, Sabitry
Publisher: name: Zenodo
Year: 2010
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Arachnida
Trombidiformes
Unionicolidae
Unionicola
Unionicola crassipes

description: Unionicola (Unionicola) crassipes (Müller, 1776)
Records from the study area: Kerala—Walter (1928); Maharashtra—Lundblad (1969); Prasad (1974).
Habitat: Ponds.
Distribution: Holarctic?
description: Published as part of Pešić, Vladimir, Chatterjee, Tapas & Bordoloi, Sabitry, 2010, A checklist of the water mites (Acari: Hydrachnidia) of India, with new records and description of one new species, pp. 1-54 in Zootaxa 2617 (1) on page 36, DOI: 10.11646/zootaxa.2617.1.1, http://zenodo.org/record/197964
title: The representation of communism in post-war British film, theatre, and art 1945-1963
Creators: name: Clulow, Jacqueline
Publisher: name: University of Liverpool Repository
Year: 2013

description: In contrast to previous cold war studies which have focussed on propaganda and the cultural contest between the USA and USSR, this thesis demonstrates how British film, theatre, and art marginalised communism, and by association political radicalism, through the reinforcement of dominant negative stereotypes. This thesis examines how largely negative portrayals of communism reflected the views of their creators: individuals and groups who were not part of the official state apparatus, but were illustrating their own perception of the communist threat. It is therefore a cultural examination of the visual portrayal of a political ideology within British film, theatre and art. Through the use of recognisable stereotypes, communism was demonised through a variety of guises: from aggressive and sinister domestic militants through to the portrayal of communism as being ‘un-British’ and a challenge to traditional values and beliefs. Although such anti-communist representations were dominant, more sympathetic portrayals gradually emerged, such as the naive, the gullible or those simply disillusioned and seeking change. Through a determined eclectic analysis of a broad range of sources from film, theatre and art this thesis will show that domestic concerns dominate in all three media. An in-depth look at the CPGB, the Artists Group and Realism, will demonstrate that British communism had no positive cultural influence within the media considered, leaving any sympathetic portrayals down to individuals. The changing fortunes of the Communist Party of Great Britain will also demonstrate how geopolitical events led to a decline in its domestic support and the continual reinforcement of negative communist portrayals. What emerges is that in the post-war years British film, theatre, and art looked with suspicion at an alien ideology largely associated with a hostile foreign power, and without any real challenge, its continued negative representation helped to establish a dominant anti-communist ideology.
Plants: the latest model system for G-protein research
Creators: name: Jones, Alan M.
name: Assmann, Sarah M.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2004

description: In humans, heterotrimeric G proteins couple stimulus perception by G-protein-coupled receptors (GPCRs) with numerous downstream effectors. By contrast, despite great complexity in their signal-transduction attributes, plants have a simpler repertoire of G-signalling components. Nonetheless, recent studies on Arabidopsis thaliana have shown the importance of plant G-protein signalling in such fundamental processes as cell proliferation, hormone perception and ion-channel regulation.
title: Optimisation of aspects of rotor blades using computational fluid dynamics
Creators: name: Johnson, Catherine
Publisher: name: University of Liverpool Repository
Year: 2013

description: This work presents a framework for the optimisation of various aspects of rotor blades in forward flight. The literature survey suggests that the quest for such a method is generating much research as more performance is obtainable from current designs. With increasing computational power and efficient methods, this can be of practical use to the helicopter industry. The proposed method employs CFD in conjunction with metamodels such as artificial neural networks (ANNs) and kriging interpolation, and a non-gradient based optimiser, in the form of genetic algorithms (GAs), for optimisation. The approach is demonstrated using several cases, including the optimisation of linear twist of rotors in hover (a steady case) and the optimisation of rotor sections in forward flight (an unsteady case); other cases include transonic aerofoils, wing and rotor tip planforms. For rotor tip planforms, first a simple rectangular rotor in hover was optimised. Then the developed method was used to optimise the anhedral and sweep of the UH60-A rotor blade in forward flight while constraining its hover performance and the final rotor optimisation was for a BERP-like rotor in forward flight, also constraining hover performance. For each case, a parameterisation method was defined, a specific objective function created using the initial CFD data and the metamodel was used for evaluating the objective function during the optimisation using the GAs. The obtained results suggest optima in agreement with engineering intuition but provide precise information about the shape of the final lifting surface and its performance. The results were checked by comparison with the Pareto subset of data and the metamodels were also validated with high-fidelity CFD data. Neither was sensitive to the employed techniques with substantial overlap between the outputs of the selected methods. The main CPU cost was associated with the population of the CFD database necessary for the metamodel. To improve this further, the Harmonic Balance alternative for obtaining the CFD data (as opposed to Time Marching) was used to increase efficiency and reduce clock time for the BERP-like tip optimisation. The novelty of this method is the use of a metamodel in conjunction with high-fidelity CFD data so that high-resolution performance improvements can be captured efficiently using a non-gradient based method.
Restoration of the cystic fibrosis transmembrane conductance regulator function by splicing modulation
Creators: name: Kerem, Batsheva
name: Chiba-Falek, Ornit
name: Pollard, Harvey B
name: Yankaskas, James R
name: Shushi, Liat
name: Randell, Scott H
name: Aviram, Micha
name: Eidelman, Ofer
name: Nissim-Rafinia, Malka
name: Ozeri, Efrat
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2004

description: A significant fraction of disease-causing mutations affects pre-mRNA splicing. These mutations can generate both aberrant and correct transcripts, the level of which varies among different patients. An inverse correlation was found between this level and disease severity, suggesting a role for splicing regulation as a genetic modifier. Overexpression of splicing factors increased the level of correctly spliced RNA, transcribed from minigenes carrying disease-causing splicing mutations. However, whether this increase could restore the protein function was unknown. Here, we demonstrate that overexpression of Htra2-β1 and SC35 increases the level of normal cystic fibrosis transmembrane conductance regulator (CFTR) transcripts in cystic-fibrosis-derived epithelial cells carrying the 3849+10 kb C → T splicing mutation. This led to activation of the CFTR channel and restoration of its function. Restoration was also obtained by sodium butyrate, a histone deacetylase inhibitor, known to upregulate the expression of splicing factors. These results highlight the therapeutic potential of splicing modulation for genetic diseases caused by splicing mutations.
title: Transversal homotopy theory of Whitney stratified manifolds
Creators: name: Smyth, Conor
Publisher: name: University of Liverpool Repository
Year: 2012

description: We modify the theory of homotopy groups to obtain invariants of Whitney stratified spaces by considering smooth maps which are transversal to all strata, and smooth homotopies through such maps. Using this idea we obtain transversal homotopy monoids with duals for any Whitney stratified space. Just as in ordinary homotopy theory we may also define higher categorical invariants of spaces. Here instead of groupoids we obtain categories with duals. We concentrate on examples involving the sphere, stratified by a point and its complement, and complex projective space stratified in a natural way. We also suggest a definition for n-category with dual, which we call a Whitney category. This is defined as a presheaf on a certain category of Whitney stratified spaces, that resticts to a sheaf on a certain subcategory. We show in detail that this definition matches the accepted notion of n-category with duals, at least for small n. It also allows us to prove a version of the Tangle Hypothesis, due to Baez and Dolan, which states that ``The n-category of framed codimension k-tangles is equivalent to the free k-tuply monoidal n-category with duals on one object."
Forms of differing degrees over number fields
Creators: name: Frei, Christopher
name: Madritsch, Manfred
Publisher: name: arXiv
Year: 2014
Subjects: subject: Number Theory (math.NT)
subject: FOS: Mathematics
subject: 11G35, 11P55, 14G05

description: Consider a system of polynomials in many variables over the ring of integers of a number field $K$. We prove an asymptotic formula for the number of integral zeros of this system in homogeneously expanding boxes. As a consequence, any smooth and geometrically integral variety $X\subseteq \mathbb{P}_K^m$ satisfies the Hasse principle, weak approximation and the Manin-Peyre conjecture, if only its dimension is large enough compared to its degree. This generalizes work of Skinner, who considered the case where all polynomials have the same degree, and recent work of Browning and Heath-Brown, who considered the case where $K=\mathbb{Q}$. Our main tool is Skinner's number field version of the Hardy-Littlewood circle method. As a by-product, we point out and correct an error in Skinner's treatment of the singular integral.
description: 23 pages; minor revision; to appear in Mathematika
Catálogo de Porotos
Creators: name: Grupo de Huerteras de Covunco
Publisher: name: ITPGRFA
Year: 2024
Subjects: PR-154
ARG
Beans
ITPGRFA-BSF
Women farmers
Traditional knowledge
Farmers
Farmers preferred varieties
Recipes

description: This catalog is the result of five years of dedication and hard work in documenting the good practices of  bean varieties, including their use and cultivation, in Zapala region of Argentina. The beginning phase of this work started in partnership with INTA extension agency in Zapala, within the framework of the National project ProHuerta and then was consolidated through the project Euroclima, and ultimately within the project “Conservación y uso sostenible de los recursos fitogenéticos locales para la alimentación y la agricultura (RFAA) para contribuir a la seguridad alimentaria de los pequeños agricultores de Argentina” funded in the 4th cycle of the Benefit Sharing Fund (BSF) of the International Treaty on Plant Genetic Resources for Food and Agriculture (ITPGRFA) of the Food and Agriculture Organization (FAO) of the United Nations (UN).

The participating groups consisted of 9 families where women were the largest representatives, many of these heads of households. They are organized as the Huerteras from Covunco group and belong to the Association of Rural Development (A.F.R.) of the area as active members.
Enhanced delegated computing using coherence
Creators: name: Barz, Stefanie
name: Dunjko, Vedran
name: Schlederer, Florian
name: Moore, Merritt
name: Kashefi, Elham
name: Walmsley, Ian A.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Quantum Physics (quant-ph)
subject: FOS: Physical sciences

description: A long-standing question is whether it is possible to delegate computational tasks securely. Recently, both a classical and a quantum solution to this problem were found. Here, we study the interplay of classical and quantum approaches and show how coherence can be used as a tool for secure delegated classical computation. We show that a client with limited computational capacity - restricted to an XOR gate - can perform universal classical computation by manipulating information carriers that may occupy superpositions of two states. Using single photonic qubits or coherent light, we experimentally implement secure delegated classical computations between an independent client and a server. The server has access to the light sources and measurement devices, whereas the client may use only a restricted set of passive optical devices to manipulate the light beams. Thus, our work highlights how minimal quantum and classical resources can be combined and exploited for classical computing.
The Body of Nursing as Seen Through the NHS Nursing Uniform
Creators: name: Hardingham, Laura
name: Heeley, Rachel
Publisher: name: FUTURESCAN
Year: 2019
Subjects: subject: 120306 Textile and Fashion Design
subject: FOS: Arts (arts, history of arts, performing arts, music)

description: Research paper presented at Futurescan 3: Intersecting Identities, Glasgow School of Art, 11th-12th November 2015.

Futurescan 3: Intersecting Identities
Edited by Helena Britt, Laura Morgan and Kerry WaltonNovember 2015
ISBN: 978 1 911217 08 4
Game theoretic approach to skeletally Dugundji and Dugundji spaces
Creators: name: Kucharski, Andrzej
name: Plewik, Szymon
name: Valov, Vesko
Publisher: name: arXiv
Year: 2015
Subjects: subject: General Topology (math.GN)
subject: FOS: Mathematics
subject: Primary 54B35, Secondary 54C10, 91A44

description: Characterizations of skeletally Dugundji spaces and Dugundji spaces are given in terms of club collections, consisting of countable families of co-zero sets. For example, a Tychonoff space $X$ is skeletally Dugundji if and only if there exists an additive $c$-club on $X$. Dugundji spaces are characterized by the existence of additive $d$-clubs.
The J-protein family: modulating protein assembly, disassembly and translocation
Creators: name: Cyr, Douglas
name: Law, Yin Chern
name: Walsh, Peter
name: Lithgow, Trevor
name: Bursać, Dejan
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2004

description: DnaJ is a molecular chaperone and the prototypical member of the J-protein family. J proteins are defined by the presence of a J domain that can regulate the activity of 70-kDa heat-shock proteins. Sequence analysis on the genome of Saccharomyces cerevisiae has revealed 22 proteins that establish four distinguishing structural features of the J domain: predicted helicity in segments I–IV, precisely placed interhelical contact residues, a lysine-rich surface on helix II and placement of the diagnostic sequence HPD between the predicted helices II and III. We suggest that this definition of the J-protein family could be used for other genome-wide studies. In addition, three J-like proteins were identified in yeast that contain regions closely resembling a J domain, but in which the HPD motif is non-conservatively replaced. We suggest that J-like proteins might function to regulate the activity of bona fide J proteins during protein translocation, assembly and disassembly.
Does the Dependence of Brand Voice on AI Restrict Freedom of Expression in Social Media?
Creators: name: Siroda, Sati
name: Surikova, Julija
Publisher: name: figshare
Year: 2023
Subjects: classificationCode: 350604
subject: Marketing communications

description: With the advent of social media and a unique brand voice, brand communications frequently employ artificial intelligence (AI) to facilitate timely interactions across the many channels of communication, and often integrate communications. This research seeks to investigate the influence of an AI-regulated brand voice on the freedom of expression (FOE) when communicating on social media.A qualitative research design is implemented based on content analysis. The research gap was identified by reviewing the role of brand voice using AI, the application of AI to monitor freedom of expression, and the study of integrating FOE in brand voice generation using AI. Various case studies were used to conclude that AI-regulated brand voice has a substantial impact on the freedom of expression on social media, which is frequently governed by privacy rules.This study establishes that a brand's communication in its social media portfolio is governed by national and international laws that regulate freedom of expression and thus have legal implications. This study suggests that AI must be fed information to prevent content such as hate speech, bullying, and so on, and produce a brand voice with checkpoints for human validation and verification to maintain general societal decorum.
title: Nonindependent mate choice in humans
Creators: name: Vakirtzis, Antonios
Publisher: name: University of Liverpool Repository
Year: 2012

description: Mate choice copying is the most studied type of nonindependent mate choice, i.e. mate choice that is influenced by the choices of other same-sex conspecifics (usually females). In copying, the probability of a male being chosen by a female ('focal' female) increases if he has previously been chosen by other females ('model' females) and decreases if he has been rejected. I critically review the non-human literature and conclude that from an evolutionary perspective copying is ill-suited to monogamous or relatively monogamous species like humans. I propose instead a related process where females are influenced not by a male‟s success at securing mates but by the quality of females that choose him. Although sometimes described as copying, this type of nonindependent mate choice is characterized by distinct evolutionary dynamics and ecological requirements, leads to different testable predictions and must therefore be urgently distinguished from mate choice copying. The term mate quality bias is suggested as an appropriate term for this phenomenon. I also report experimental studies that presented female raters with both static and video images of model females and their supposed partners. The two main findings to emerge from these experiments are a) the main - and perhaps the only - relevant cue in the model female is attractiveness and b) experimental studies can suffer from reduced external validity and need to be supplemented with non-experimental approaches. In line with this latter finding, I report one of the first non-experimental studies of nonindependent mate choice in humans. This involved the administration of a novel questionnaire to a large sample (n=401) of male and female undergraduates. The results of this study provide strong support support for nonindependent mate choice in humans a) being an empirical reality and b)influencing female, but not male choice. Finally, I report two experimental studies which examined how a man's partner influences male-male assessment and competition. The first used the dictator and ultimatum games to examine if offers made to male recipients were influenced by the attractiveness of the recipient‟s partner. The second used the Wason selection task to examine whether male subjects' cheater detection faculties are influenced by the attractiveness of the target male‟s partner (used here as a proxy for dominance). Although the results were generally in the expected direction, experimental manipulation of female partner attractiveness did not significantly affect male raters' perceptions of, and behaviour towards, the target male. The thesis concludes with a critical evaluation of the results obtained herein and suggestions for future research.
Structure of Uniformly Continuous Quantum Markov Semigroups
Creators: name: Deschamps, Julien
name: Fagnola, Franco
name: Sasso, Emanuela
name: Umanita', Veronica
Publisher: name: arXiv
Year: 2014
Subjects: subject: Mathematical Physics (math-ph)
subject: FOS: Physical sciences
subject: 82C10, 47D06, 46L55

description: The structure of uniformly continuous quantum Markov semigroups with atomic decoherence-free subalgebra is established providing a naturaldecomposition of a Markovian open quantum system into its noiseless (decoherence-free) and irreducible (ergodic) components. This leads to a new characterisation of the structure of invariant states and a new method for finding decoherence-free subsystems and subspaces. Examples are presented to illustrate these results.
Tudor, MBT and chromo domains gauge the degree of lysine methylation
Creators: name: Espejo, Alexsandra
name: Kim, Jeesun
name: Xia, Li
name: Lake, Aimee
name: Krishna, Murli
name: Zhang, Yi
name: Daniel, Jeremy
name: Bedford, Mark T
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2006

description: The post-translational modification of histones regulates many cellular processes, including transcription, replication and DNA repair. A large number of combinations of post-translational modifications are possible. This cipher is referred to as the histone code. Many of the enzymes that lay down this code have been identified. However, so far, few code-reading proteins have been identified. Here, we describe a protein-array approach for identifying methyl-specific interacting proteins. We found that not only chromo domains but also tudor and MBT domains bind to methylated peptides from the amino-terminal tails of histones H3 and H4. Binding specificity observed on the protein-domain microarray was corroborated using peptide pull-downs, surface plasma resonance and far western blotting. Thus, our studies expose tudor and MBT domains as new classes of methyl-lysine-binding protein modules, and also demonstrates that protein-domain microarrays are powerful tools for the identification of new domain types that recognize histone modifications.
Practicing What We Preach
Creators: name: Shah, Karen
Publisher: name: FUTURESCAN
Year: 2019
Subjects: subject: 120306 Textile and Fashion Design
subject: FOS: Arts (arts, history of arts, performing arts, music)

description: Research paper presented at Futurescan 3: Intersecting Identities, Glasgow School of Art, 11th-12th November 2015.

Futurescan 3: Intersecting Identities
Edited by Helena Britt, Laura Morgan and Kerry WaltonNovember 2015
ISBN: 978 1 911217 08 4
Africasia mahadensis Cook 1967
Creators: name: Pešić, Vladimir
name: Chatterjee, Tapas
name: Bordoloi, Sabitry
Publisher: name: Zenodo
Year: 2010
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Arachnida
Trombidiformes
Arrenuridae
Africasia
Africasia mahadensis

description: Africasia mahadensis Cook, 1967
Records from the study area: Maharashtra—Cook (1967); Prasad (1974).
Habitat: Streams.
Distribution: India.
description: Published as part of Pešić, Vladimir, Chatterjee, Tapas & Bordoloi, Sabitry, 2010, A checklist of the water mites (Acari: Hydrachnidia) of India, with new records and description of one new species, pp. 1-54 in Zootaxa 2617 (1) on page 36, DOI: 10.11646/zootaxa.2617.1.1, http://zenodo.org/record/197964
Africasia navina Cook 1967
Creators: name: Pešić, Vladimir
name: Chatterjee, Tapas
name: Bordoloi, Sabitry
Publisher: name: Zenodo
Year: 2010
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Arachnida
Trombidiformes
Arrenuridae
Africasia
Africasia navina

description: Africasia navina Cook, 1967
Records from the study area: Kerala—Cook (1967); Prasad (1974).
Habitat: Mountain streams.
Distribution: India.
description: Published as part of Pešić, Vladimir, Chatterjee, Tapas & Bordoloi, Sabitry, 2010, A checklist of the water mites (Acari: Hydrachnidia) of India, with new records and description of one new species, pp. 1-54 in Zootaxa 2617 (1) on page 36, DOI: 10.11646/zootaxa.2617.1.1, http://zenodo.org/record/197964
The Problems with the Extended Sleeve Styles of Post-War Dress
Creators: name: Birbidge, Helen
Publisher: name: FUTURESCAN
Year: 2019
Subjects: subject: 120306 Textile and Fashion Design
subject: FOS: Arts (arts, history of arts, performing arts, music)

description: Research paper presented at Futurescan 3: Intersecting Identities, Glasgow School of Art, 11th-12th November 2015.

Futurescan 3: Intersecting Identities
Edited by Helena Britt, Laura Morgan and Kerry WaltonNovember 2015
ISBN: 978 1 911217 08 4
Bernoulli Convolutions and 1D Dynamics
Creators: name: Kempton, Tom
name: Persson, Tomas
Publisher: name: arXiv
Year: 2015
Subjects: subject: Dynamical Systems (math.DS)
subject: FOS: Mathematics
subject: 37E05, 37M25

description: We describe a family $ϕ_λ$ of dynamical systems on the unit interval which preserve Bernoulli convolutions. We show that if there are parameter ranges for which these systems are piecewise convex, then the corresponding Bernoulli convolution will be absolutely continuous with bounded density. We study the systems $ϕ_λ$ and give some numerical evidence to suggest values of $λ$ for which $ϕ_λ$ may be piecewise convex.
description: 18 pages
RNA steady-state defects in myotonic dystrophy are linked to nuclear exclusion of SHARP
Creators: name: Comai, Lucio
name: Marjoram, Paul
name: Mohammadzadeh, Robabeh
name: Reddy, Sita
name: Dansithong, Warunee
name: Jog, Sonali P
name: Kwok, Yukwah
name: Fry, Rebecca C
name: Tring, Stephanie
name: Paul, Sharan
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: We describe a new mechanism by which CTG tract expansion affects myotonic dystrophy (DM1). Changes to the levels of a panel of RNAs involved in muscle development and function that are downregulated in DM1 are due to aberrant localization of the transcription factor SHARP (SMART/HDAC1-associated repressor protein). Mislocalization of SHARP in DM1 is consistent with increased CRM1-mediated export of SHARP to the cytoplasm. A direct link between CTG repeat expression and SHARP mislocalization is demonstrated as expression of expanded CTG repeats in normal cells recapitulates cytoplasmic SHARP localization. These results demonstrate a role for the inactivation of SHARP transcription in DM1 biology.
Tumour suppressor SIRT3 deacetylates and activates manganese superoxide dismutase to scavenge ROS
Creators: name: Zhang, Jinye
name: Guan, Kun-Liang
name: Chen, Yaohui
name: Zhao, Shimin
name: Lei, Qunying
name: Xiong, Yue
name: Lin, Yan
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: Tumour suppressor SIRT3 deacetylates and activates manganese superoxide dismutase to scavenge ROSMitochondria manganese superoxide dismutase (SOD2) is a major antioxidant enzyme associated with several diseases. This study shows that SOD2 is inhibited by acetylation and activated by SIRT3-mediated deacetylation in response to reactive oxygen species (ROS).Mitochondria manganese superoxide dismutase (SOD2) is an important antioxidant enzyme, deficiency of which is associated with various human diseases. The known primary regulation of SOD2 is through transcriptional activation. Here, we report that SOD2 is acetylated at Lys 68 and that this acetylation decreases SOD2 activity. Mitochondrial deacetylase SIRT3 binds to, deacetylates and activates SOD2. Increase of reactive oxygen species (ROS) levels stimulates SIRT3 transcription, leading to SOD2 deacetylation and activation. SOD2-mediated ROS reduction is synergistically increased by SIRT3 co-expression, but is cancelled by SIRT3 depletion. These results reveal a new post-translational regulation of SOD2 by means of acetylation and SIRT3-dependent deacetylation in response to oxidative stress.
title: An ultrastructural study of the role of clathrin as an inter-microtubule bridge in kinetochore fibres
Creators: name: Booth, Daniel
Publisher: name: University of Liverpool Repository
Year: 2012

description: The mitotic spindle is required for the accurate segregation of an equal number of chromosomes, into two separate daughter cells. The movement of chromosomes is carried out by kinetochore fibres (K-fibres) of the spindle apparatus, these extend from the cell pole and terminate at attachment on the kinetochore. K-fibres are composed of bundles of individual kinetochore microtubules (kMTs). Microtubule (MT) bundling is coordinated by inter-MT bridges, which are thought to promote MT stability and organisation. The inter-MT bridges in K-fibres have never been fully characterised. Clathrin is a triskelion-structured protein with a well-characterised role in membrane trafficking, where it forms the ‘coat’ of clathrin-coated vesicles. More recently clathrin has been shown to have an unrelated function during mitosis: a period of the cell cycle when membrane trafficking is dormant. The depletion of clathrin by RNAi results in a number of mitotic defects, all of which are a consequence of destabilised K-fibres. It has been proposed that a TACC3/ch-TOG/clathrin complex physically cross-braces kMTs to stabilise K-fibres. This project set-up correlative light electron microscopy (CLEM) methods allowing mitotic, clathrin-depleted cells to be analysed from the level of light microscopy to electron microcopy. A dual-axis ultrastructural methodology was also developed allowing K-fibre bundles and inter-MT bridges to be measured. This project used CLEM to reveal that a clathrin complex functions as a population of short inter-MT bridges in K-fibres. The depletion of clathrin or TACC3 resulted in: 1) a loss of kMTs, 2) a decrease in kMT organisation and 3) a loss of a population of short inter-kMT bridges. Immunogold labelling confirmed that clathrin was a component of the inter-kMT bridge. The literature typically refer to inter-MT bridges as electron dense ‘struts’ that cross-link MTs, however, a few classic EM studies have described bridges as ‘filamentous’ with ‘multiple projections’. A highly resolved view of bridge structures was sought. Using high-pressure freezing with freeze-substitution, tomography and 3D rendering, this project revealed that a novel structure was present that cross-linked kMTs, we have termed this structure ‘inter-kMT mesh’. The mesh was found exclusively between kMTs and appeared to promote kMT organisation through multiple contacts with MTs. The mesh responded to manipulated levels of TACC3 expression, resulting in disorganised K-fibre bundles, misshapen kMTs and an enhanced electron-dense inter-kMT mesh. Collectively, these new data contribute to the existing knowledge of inter-MT cross-linkers in K-fibres by providing novel insights into their structure and how they confer kMT stability and organisation.
title: Symmetries of unimodal singularities and complex hyperbolic reflection groups
Creators: name: Haddley, Joel A
Publisher: name: University of Liverpool Repository
Year: 2011

description: In search of discrete complex hyperbolic reflection groups in a singularity context, we consider cyclic symmetries of the 14 exceptional unimodal function singularities. In the 3-variable case, we classify all the symmetries for which the restriction of the intersection form of an invariant Milnor fibre to a character subspace has positive signature 1, and hence the corresponding equivariant monodromy is a reflection subgroup of U(k − 1,1). For such subspaces, we construct distinguished vanishing bases and their Dynkin diagrams. For k = 2, the projectivised hyperbolic monodromy is a triangle group of the Poincaré disk. For k = 3, we identify some of the projectivised monodromy groups within a recently published survey by J. R. Parker.
A simple approach to the numerical simulation with trimmed CAD surfaces
Creators: name: Beer, Gernot
name: Marussig, Benjamin
name: Zechner, Jürgen
Publisher: name: arXiv
Year: 2015
Subjects: subject: Numerical Analysis (math.NA)
subject: FOS: Mathematics

description: In this work a novel method for the analysis with trimmed CAD surfaces is presented. The method involves an additional mapping step and the attraction stems from its sim- plicity and ease of implementation into existing Finite Element (FEM) or Boundary Element (BEM) software. The method is first verified with classical test examples in structural mechanics. Then two practical applications are presented one using the FEM, the other the BEM, that show the applicability of the method.
description: 20 pages and 16 figures
Africasia pinguipalpis Cook 1967
Creators: name: Pešić, Vladimir
name: Chatterjee, Tapas
name: Bordoloi, Sabitry
Publisher: name: Zenodo
Year: 2010
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Arachnida
Trombidiformes
Arrenuridae
Africasia
Africasia pinguipalpis

description: Africasia pinguipalpis Cook, 1967
Records from the study area: Kerala—Cook (1967); Prasad (1974).
Habitat: Mountain streams.
Distribution: India.
description: Published as part of Pešić, Vladimir, Chatterjee, Tapas & Bordoloi, Sabitry, 2010, A checklist of the water mites (Acari: Hydrachnidia) of India, with new records and description of one new species, pp. 1-54 in Zootaxa 2617 (1) on page 36, DOI: 10.11646/zootaxa.2617.1.1, http://zenodo.org/record/197964
Comment on "Generalized exclusion processes: Transport coefficients"
Creators: name: Becker, T.
name: Nelissen, K.
name: Cleuren, B.
name: Partoens, B.
name: Broeck, C. Van den
Publisher: name: arXiv
Year: 2014
Subjects: subject: Statistical Mechanics (cond-mat.stat-mech)
subject: FOS: Physical sciences

description: In a recent paper Arita et al. [Phys. Rev. E 90, 052108 (2014)] consider the transport properties of a class of generalized exclusion processes. Analytical expressions for the transport-diffusion coefficient are derived by ignoring correlations. It is claimed that these expressions become exact in the hydrodynamic limit. In this Comment, we point out that (i) the influence of correlations upon the diffusion does not vanish in the hydrodynamic limit, and (ii) the expressions for the self- and transport diffusion derived by Arita et al. are special cases of results derived in [Phys. Rev. Lett. 111, 110601 (2013)].
description: (citation added, published version)
The Wear Project: Identity and Clothing in Relation to Costume Design and Education
Creators: name: Malik, Nadia
Publisher: name: FUTURESCAN
Year: 2019
Subjects: subject: 120306 Textile and Fashion Design
subject: FOS: Arts (arts, history of arts, performing arts, music)

description: Research paper presented at Futurescan 3: Intersecting Identities, Glasgow School of Art, 11th-12th November 2015.

Futurescan 3: Intersecting Identities
Edited by Helena Britt, Laura Morgan and Kerry WaltonNovember 2015
ISBN: 978 1 911217 08 4
Cyclic-di-GMP and cyclic-di-AMP activate the NLRP3 inflammasome
Creators: name: Cambier, John C
name: Abdul-Sater, Ali A
name: Jin, Lei
name: Koller, Beverly H
name: Beaucage, Serge L
name: Tattoli, Ivan
name: Ting, Jenny P -Y
name: Allen, Irving C
name: Levi, Assaf
name: Schindler, Christian
name: Grajkowski, Andrzej
name: Fitzgerald, Katherine A
name: Girardin, Stephen E
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2013

description: The cyclic dinucleotides 3'-5'diadenylate (c-diAMP) and 3'-5' diguanylate (c-diGMP) are important bacterial second messengers that have recently been shown to stimulate the secretion of type I Interferons (IFN-Is) through the c-diGMP-binding protein MPYS/STING. Here, we show that physiologically relevant levels of cyclic dinucleotides also stimulate a robust secretion of IL-1β through the NLRP3 inflammasome. Intriguingly, this response is independent of MPYS/STING. Consistent with most NLRP3 inflammasome activators, the response to c-diGMP is dependent on the mobilization of potassium and calcium ions. However, in contrast to other NLRP3 inflammasome activators, this response is not associated with significant changes in mitochondrial potential or the generation of mitochondrial reactive oxygen species. Thus, cyclic dinucleotides activate the NLRP3 inflammasome through a unique pathway that could have evolved to detect pervasive bacterial pathogen-associated molecular patterns associated with intracellular infections.
Fashion Education in Brazil
Creators: name: Neira, Luz Garcia
Publisher: name: FUTURESCAN
Year: 2019
Subjects: subject: 120306 Textile and Fashion Design
subject: FOS: Arts (arts, history of arts, performing arts, music)

description: Research paper presented at Futurescan 3: Intersecting Identities, Glasgow School of Art, 11th-12th November 2015.

Futurescan 3: Intersecting Identities
Edited by Helena Britt, Laura Morgan and Kerry WaltonNovember 2015
ISBN: 978 1 911217 08 4
Brahma is required for cell cycle arrest and late muscle gene expression during skeletal myogenesis
Creators: name: Dall'Agnese, A.
name: Coutinho Toto, P.
name: Albini, S.
name: Caruso, M.
name: Puri, P. L.
name: Malecova, B.
name: Cenciarelli, C.
name: Felsani, A.
name: Bultman, S. J.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2015

description: Although the two catalytic subunits of the SWI/SNF chromatin-remodeling complex—Brahma (Brm) and Brg1—are almost invariably co-expressed, their mutually exclusive incorporation into distinct SWI/SNF complexes predicts that Brg1- and Brm-based SWI/SNF complexes execute specific functions. Here, we show that Brg1 and Brm have distinct functions at discrete stages of muscle differentiation. While Brg1 is required for the activation of muscle gene transcription at early stages of differentiation, Brm is required for Ccnd1 repression and cell cycle arrest prior to the activation of muscle genes. Ccnd1 knockdown rescues the ability to exit the cell cycle in Brm-deficient myoblasts, but does not recover terminal differentiation, revealing a previously unrecognized role of Brm in the activation of late muscle gene expression independent from the control of cell cycle. Consistently, Brm null mice displayed impaired muscle regeneration after injury, with aberrant proliferation of satellite cells and delayed formation of new myofibers. These data reveal stage-specific roles of Brm during skeletal myogenesis, via formation of repressive and activatory SWI/SNF complexes.
Free energy landscape and characteristic forces for the initiation of DNA unzipping
Creators: name: Mentes, Ahmet
name: Florescu, Ana Maria
name: Brunk, Elizabeth
name: Wereszczynski, Jeff
name: Joyeux, Marc
name: Andricioaei, Ioan
Publisher: name: arXiv
Year: 2015
Subjects: subject: Biological Physics (physics.bio-ph)
subject: Soft Condensed Matter (cond-mat.soft)
subject: Biomolecules (q-bio.BM)
subject: FOS: Physical sciences
subject: FOS: Biological sciences

description: DNA unzipping, the separation of its double helix into single strands, is crucial in modulating a host of genetic processes. Although the large-scale separation of double-stranded DNA has been studied with a variety of theoretical and experimental techniques, the minute details of the very first steps of unzipping are still unclear. Here, we use atomistic molecular dynamics (MD) simulations, coarse-grained simulations and a statistical-mechanical model to study the initiation of DNA unzipping by an external force. The calculation of the potential of mean force profiles for the initial separation of the first few terminal base pairs in a DNA oligomer reveal that forces ranging between 130 and 230 pN are needed to disrupt the first base pair, values of an order of magnitude larger than those needed to disrupt base pairs in partially unzipped DNA. The force peak has an "echo," of approximately 50 pN, at the distance that unzips the second base pair. We show that the high peak needed to initiate unzipping derives from a free energy basin that is distinct from the basins of subsequent base pairs because of entropic contributions and we highlight the microscopic origin of the peak. Our results suggest a new window of exploration for single molecule experiments.
description: 25 pages, 6 figures , Accepted for publication in Biophysical Journal
Open to Change: Slowing Down to Explore and Innovate
Creators: name: Redmore, Nicola
Publisher: name: FUTURESCAN
Year: 2019
Subjects: subject: 120306 Textile and Fashion Design
subject: FOS: Arts (arts, history of arts, performing arts, music)

description: Research paper presented at Futurescan 3: Intersecting Identities, Glasgow School of Art, 11th-12th November 2015.

Futurescan 3: Intersecting Identities
Edited by Helena Britt, Laura Morgan and Kerry WaltonNovember 2015
ISBN: 978 1 911217 08 4
Africasia rucira Cook 1967
Creators: name: Pešić, Vladimir
name: Chatterjee, Tapas
name: Bordoloi, Sabitry
Publisher: name: Zenodo
Year: 2010
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Arachnida
Trombidiformes
Arrenuridae
Africasia
Africasia rucira

description: Africasia rucira Cook, 1967
Records from the study area: Kerala—Cook (1967).
Habitat: Mountain streams.
Distribution: India.
description: Published as part of Pešić, Vladimir, Chatterjee, Tapas & Bordoloi, Sabitry, 2010, A checklist of the water mites (Acari: Hydrachnidia) of India, with new records and description of one new species, pp. 1-54 in Zootaxa 2617 (1) on page 36, DOI: 10.11646/zootaxa.2617.1.1, http://zenodo.org/record/197964
Cutting your Coat According to your Cloth: Maintaining Technical Skills and Understanding within Tighter Budgets, through Connecting Communities in Mutually Beneficial and Support Skills Exchange
Creators: name: Richardson, Lindy
Publisher: name: FUTURESCAN
Year: 2019
Subjects: subject: 120306 Textile and Fashion Design
subject: FOS: Arts (arts, history of arts, performing arts, music)

description: Research paper presented at Futurescan 3: Intersecting Identities, Glasgow School of Art, 11th-12th November 2015.

Futurescan 3: Intersecting Identities
Edited by Helena Britt, Laura Morgan and Kerry WaltonNovember 2015
ISBN: 978 1 911217 08 4
title: Examining the dynamics of the proteome using stable isotopes as metabolic tracers
Creators: name: Pennington, Andrea
Publisher: name: University of Liverpool Repository
Year: 2011

description: The primary objective of this study was to examine the turnover of different proteins using stable isotopes as metabolic tracers. Two different labelling methods were used during the course of this investigation; [2H]20 and [2H8] valine. Heavy water labelling resulted in the incorporation of deuterium into non-essential amino acids, and was primarily used to assess the turnover of mouse urinary proteins (MUPs). MUP’s showed rapid deuterium incorporation, with equilibrium reached at day two, indicating high turnover. This result was anticipated as it had already been established that MUPs are made by the liver and rapidly excreted in the urine. Heavy valine was used to perform a more comprehensive study of turnover. Mice were provided with a diet in which 50% of the valine was in heavy. Heavy valine was incorporated into proteins via protein synthesis and labelling was monitored using mass spectrometry. As with the heavy water labelling, MUP’s showed rapid labelling, with other proteins showing a range of different labelling rates. The most interesting observation in the heavy valine study was made when monitoring label incorporation in sperm and seminal vesicle secretion (SVS) proteins. SVS proteins showed rapid label incorporation from day two onwards, indicating high protein turnover. However the sperm proteins showed a distinct delay in labelling relative to the SVS proteins. It is hoped that this delay in the labelling of sperm proteins relative to SVS proteins could be exploited to selectively label specific proteins.
PARD3 induces TAZ activation and cell growth by promoting LATS1 and PP1 interaction
Creators: name: Lei, Q.-Y.
name: Sun, Y.-P.
name: Lv, X.-B.
name: Wang, Z.
name: Guan, K.-L.
name: Liu, C.-Y.
name: Xiong, Y.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2015

description: The Hippo pathway plays a major role in organ size control, and its dysregulation contributes to tumorigenesis. The major downstream effectors of the Hippo pathway are the YAP/TAZ transcription co-activators, which are phosphorylated and inhibited by the Hippo pathway kinase LATS1/2. Here, we report a novel mechanism of TAZ regulation by the tight junction protein PARD3. PARD3 promotes the interaction between PP1A and LATS1 to induce LATS1 dephosphorylation and inactivation, therefore leading to dephosphorylation and activation of TAZ. The cytoplasmic, but not the tight junction complex associated, PARD3 is responsible for TAZ regulation. Our study indicates a potential molecular basis for cell growth-promoting function of PARD3 by modulating the Hippo pathway signaling in response to cell contact and cell polarity signals.
title: Novel biomarkers for the diagnosis of pancreatic ductal adenocarcinoma: the proteomic approach
Creators: name: Tang, Joseph Man Fung
Publisher: name: University of Liverpool Repository
Year: 2012

description: Background: Pancreatic ductal adenocarcinoma (PDAC) is a disease of late presentation where the majority of patients present with non-specific symptoms and advanced disease. Current guidelines recommend that patients presenting with symptoms of suggestive of PDAC should be investigated by Contrast-Enhanced Computed Tomography (CE-CT). However, the radiographic features are often similar to benign diseases such as chronic pancreatitis (CP). Evidently, there is a need for a novel diagnostic biomarker, which can accurately identify patients with PDAC thereby reducing the number of otherwise unnecessary invasive procedures. Aim: The current thesis aimed to determine the potential of a number of serum proteins as diagnostic markers of PDAC. Method: Two approaches for the discovery and validation of diagnostic markers of PDAC were employed. In Chapter 2, the serum expression of three iTRAQ- Mass Spectrometry identified proteins (vitamin d-binding protein [VDBP], retinol-binding protein 4 [RBP-4], and fibronectin [FINC]) were validated by western blotting in a three-phased study consisting of 20, 60, and 120 serum samples. Their diagnostic potentials as individual and combined markers were assessed statistically. In Chapter 3, the serum concentrations of 27 cytokines, chemokines, and growth factors (CCGFs) in 90 PDAC and 90 controls were quantified using the multiplex cytokines assay and the potential of individual CCGFs for the diagnosis of PDACs were assessed. One-hundred and twenty serum samples were randomly allocated to discovery where stepwise regression was used to select independent CCGF markers of PDAC. These were then combined into a single marker and the diagnostic accuracy for PDAC assessed. Finally, validation utilised the remaining sixty samples to investigate the accuracy of the combined CCGF marker for the diagnosis of PDAC. Results: Results from Chapter 2 showed that the serum concentrations of VDBP, RBP-4, and FINC were significant decreased in PDAC with ROC-AUCs of >0.74 against CP and healthy volunteers (HC). However, their diagnostic accuracies were decreased (ROC-AUC 0.95. Conclusion: A combined marker consisting of four selected CCGFs may serve as a potential diagnostic marker for pancreatic cancer and its use in the clinical setting may improve the current diagnostic process.