title: ÐÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð´ÐµÑÐµÑÐ¼Ð¸Ð½Ð°Ð½Ñ Ð¸ ÑÐµÐ½Ð¾ÑÐ¸Ð¿Ð¸ÑÐµÑÐºÐ¸Ñ Ñ Ð°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸Ðº Ð³Ð¸Ð¿ÐµÑÐ²Ð¸ÑÑÐ»ÐµÐ½ÑÐ½Ð¾ÑÑÐ¸ Klebsiella pneumoniae
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Determination of genetic determinants and phenotypic characteristics of Klebsiella pneumoniae hypervirulence
Creators: name: Ð¡Ð²ÐµÑÐ»Ð¾Ð², ÐÐ°Ð½Ð¸Ð¸Ð»
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Ð³Ð¸Ð¿ÐµÑÐ²Ð¸ÑÑÐ»ÐµÐ½ÑÐ½Ð¾ÑÑÑ
k. pneumoniae
ÑÐ°ÐºÑÐ¾ÑÑ Ð²Ð¸ÑÑÐ»ÐµÐ½ÑÐ½Ð¾ÑÑÐ¸
ÑÑÑÐ¸Ð½Ð³-ÑÐµÑÑ
Ð³Ð¸Ð¿ÐµÑÐ¼ÑÐºÐ¾Ð¸Ð´Ð½Ð¾ÑÑÑ
hypervirulence
virulence factors
string-test
hypermucoviscous

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«ÐÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð´ÐµÑÐµÑÐ¼Ð¸Ð½Ð°Ð½Ñ Ð¸ ÑÐµÐ½Ð¾ÑÐ¸Ð¿Ð¸ÑÐµÑÐºÐ¸Ñ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸Ðº Ð³Ð¸Ð¿ÐµÑÐ²Ð¸ÑÑÐ»ÐµÐ½ÑÐ½Ð¾ÑÑÐ¸ Klebsiella pneumoniaÐµÂ» (K. pneumoniae). ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ Ð³ÐµÐ½Ð¾Ð² Ð²Ð¸ÑÑÐ»ÐµÐ½ÑÐ½Ð¾ÑÑÐ¸ ÐºÐ»Ð¸Ð½Ð¸ÑÐµÑÐºÐ¸Ñ Ð¸Ð·Ð¾Ð»ÑÑÐ¾Ð² K. pneumoniae, Ð° ÑÐ°ÐºÐ¶Ðµ ÑÐµÐ½Ð¾ÑÐ¸Ð¿Ð¸ÑÐµÑÐºÐ¸Ñ ÑÐ²Ð¾Ð¹ÑÑÐ² Ð³Ð¸Ð¿ÐµÑÐ²Ð¸ÑÑÐ»ÐµÐ½ÑÐ½ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð².ÐÐ°Ð´Ð°ÑÐ¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ ÑÐµÐ½Ð¾ÑÐ¸Ð¿Ð¸ÑÐµÑÐºÐ¸Ñ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸Ðº Ð²Ð¸ÑÑÐ»ÐµÐ½ÑÐ½Ð¾ÑÑÐ¸ Ñ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð² K. pneumoniae, ÑÐ¾ÑÑÐ°Ð²Ð»ÐµÐ½Ð¸Ðµ Ð¿ÐµÑÐµÑÐ½Ñ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð´ÐµÑÐµÑÐ¼Ð¸Ð½Ð°Ð½Ñ, ÑÐ²ÑÐ·Ð°Ð½Ð½ÑÑ Ñ Ð³Ð¸Ð¿ÐµÑÐ²Ð¸ÑÑÐ»ÐµÐ½ÑÐ½Ð¾ÑÑÑ, Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð´ÐµÑÐµÑÐ¼Ð¸Ð½Ð°Ð½Ñ Ð²Ð¸ÑÑÐ»ÐµÐ½ÑÐ½Ð¾ÑÑÐ¸ Ñ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð² ÐºÐ»ÐµÐ±ÑÐ¸ÐµÐ»Ð» Ð¸ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð¼ÑÐ»ÑÑÐ¸Ð¿Ð»ÐµÐºÑÐ½Ð¾Ð¹ ÐÐ¦Ð  Ð´Ð»Ñ Ð¾Ð´Ð½Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¸Ñ Ð³ÐµÐ½Ð¾Ð², ÑÐ²ÑÐ·Ð°Ð½Ð½ÑÑ Ñ Ð³Ð¸Ð¿ÐµÑÐ²Ð¸ÑÑÐ»ÐµÐ½ÑÐ½ÑÐ¼ ÑÐµÐ½Ð¾ÑÐ¸Ð¿Ð¾Ð¼.ÐÐ±ÑÐµÐºÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â 65 ÐºÐ»Ð¸Ð½Ð¸ÑÐµÑÐºÐ¸Ñ Ð¸Ð·Ð¾Ð»ÑÑÐ¾Ð² K. pneumoniae Ð¸Ð· Ð¿ÑÐ¾Ð± Ð±Ð¸Ð¾Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ð° Ð¾Ñ Ð³Ð¾ÑÐ¿Ð¸ÑÐ°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ Ð¿Ð°ÑÐ¸ÐµÐ½ÑÐ¾Ð² Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³Ð°. Ð Ð°Ð±Ð¾ÑÐ° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ Ð¤ÐÐ£Ð ÐÐÐÐ­Ð Ð¸Ð¼. ÐÐ°ÑÑÐµÑÐ°. ÐÐµÑÐ¾Ð´Ð°Ð¼Ð¸ Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð¸Ð·Ð¾Ð»ÑÑÐ¾Ð² ÑÐ²Ð»ÑÐ»Ð¸ÑÑ: Â«Ð¡ÑÑÐ¸Ð½Ð³-ÑÐµÑÑÂ», ÐÐ¦Ð -Ð´ÐµÑÐµÐºÑÐ¸Ñ Ð³ÐµÐ½Ð¾Ð² Ð²Ð¸ÑÑÐ»ÐµÐ½ÑÐ½Ð¾ÑÑÐ¸ Ð¸ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÐ·.ÐÐ¾ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ°Ð¼ ÑÐ°Ð±Ð¾ÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¾ 65 ÐºÐ»Ð¸Ð½Ð¸ÑÐµÑÐºÐ¸Ñ Ð¸Ð·Ð¾Ð»ÑÑÐ¾Ð² K. pneumoniae Ð¸Ð· Ð¿ÑÐ¾Ð± Ð±Ð¸Ð¾Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ð° Ð¾Ñ Ð³Ð¾ÑÐ¿Ð¸ÑÐ°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ Ð¿Ð°ÑÐ¸ÐµÐ½ÑÐ¾Ð² Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³Ð° Ð¸ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð° Ð¼ÑÐ»ÑÑÐ¸Ð¿Ð»ÐµÐºÑÐ½Ð°Ñ ÐÐ¦Ð  Ð´Ð»Ñ Ð¾Ð´Ð½Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¸Ñ ÑÑÐµÑ Ð³ÐµÐ½Ð¾Ð² Ð²Ð¸ÑÑÐ»ÐµÐ½ÑÐ½Ð¾ÑÑÐ¸ uni-rmpÐ, iroB, iutA.
description: The subject of the graduate qualification work is "Determination of genetic determinants and phenotypic characteristics of Klebsiella pneumoniae hypervirulence". This work is devoted to the study of various virulence genes of K. pneumoniae clinical isolates, as well as the phenotypic properties of hypervirulent strainsThe tasks of the study: To make a research of phenotypic virulence characteristics in isolated K. pneumoniae strains, to make a list of genetic determinants associated with hypervirulence, to study genetic determinants of virulence in isolated Klebsiella strains and to conduct a multiplex PCR for simultaneous detection of genes associated with hypervirulent phenotype. The object of the study is 65 clinical K. pneumoniae isolates from biomaterial samples from hospitalized patients in St. Petersburg.The work was carried out on the basis of the Saint-Petersburg Pasteur Institute The methods of studying isolates were: "String-test", PCR detection of virulence genes and electrophoresis.In the course of the research, 65 clinical K. pneumoniae isolates were studied from biomaterial samples from hospitalized patients in St. Petersburg and multiplex PCR was developed for simultaneous detection of three virulence genes - uni-rmpÐ, iroB, iutA.