cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Screening cellular deformability for cancer prognosis and treatment
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Cell mechanical phenotype or ‘mechanotype’ is emerging as a distinguishing feature of malignant cells. However, what causes this shift in the mechanical properties of cancer cells remains elusive. Here we propose to establish the relationship between cell malignant characteristics and mechanotype by determining the deformability of a panel of ovarian carcinoma cells derived from tumors of varying grades. Deformability will be assayed by measuring the timescale for cells to passage through micron-scale constrictions in the channels of a microfluidic device. We will correlate the deformation time data with cell invasive potential using invasion and migration assays, as well as proliferation rates. To decipher the molecular origins of the cellular deformability, we will measure transcript and protein levels for putative mechanical regulators, including actin, vimentin, and nuclear lamin proteins. To determine any alterations in subcellular structure, we will also perform confocal imaging of immunostained cells. By quantitative image analysis, we will evaluate the nuclear-to-cytoplasmic ratio, which we hypothesize could impact the deformation time of cells through tight spaces. To further establish the extent to which we can tune cell passage through micron-scale gaps, we will treat cells with microRNAs to impair or enhance their pathologic characteristics. These experiments will define mechanotyping as a viable approach for screening small molecules that hinder metastasis using a simple microfluidic assay. More broadly, our work will shed light on the molecular mechanism of the altered structural and mechanical properties of individual cancer cells, and the relationship to their malignant behavior.
Conservation and recreation In Camden county (phase 1)
Creators: name: No Name Supplied
Publisher: name: No Publisher Supplied
Year: 1963

description: This study is an integral part of the comprehensive plan being prepared for Camden county and is intended to evaluate the status of existing parks and recreational facilities in the county. In the course of preparing this section of the plan, preliminary surveys and studies were conducted, covering such aspects as the need and demand for recreational facilities and the preferences and opinions of county residents who use the facilities. The report also explores the potentialities of the county's open lands and attempts to draw recommendations for a course of action to improve the county's system of parks.
Eleusine tristachya Lam.
Creators: name: Jeanmonod, Daniel
Publisher: name: Zenodo
Year: 2015
Subjects: Biodiversity
Taxonomy
Plantae
Tracheophyta
Liliopsida
Poales
Poaceae
Eleusine
Eleusine tristachya

description: Eleusine tristachya (Lam.) Lam.
− Secteur Plaine Orientale, commune de Lucciana, route 507 en direction de l’aéroport de Bastia-Poretta, au lieudit Pietrabiu, du côté N de la route devant les pépinières «Fourny», quelques pieds, 17.9.201 4, Delage, A. cbnc. observation; commune de Santa-Lucia-di-Moriani, en plusieurs points le long de la D109, entre les lieuxdits Prunete et Petrignani, 17 m, 17.7.201 4, Delage, A. cbnc observation; commune de San-Nicolao, bord de la D330 à l’W de Piedigrado, 120 m à l’E du pont coté 236, 17.7.201 4, Delage, A. s.n. (Hb. CBNC); commune de Prunelli-di-Fium’Orbu, le long de RN198 au S de Ghisonaccia, sur le terre-plein central de la route au sud immédiat du rond-point de Migliacciaru, 10 m, 1.9.201 4, Delage, A. cbnc observation.
Ces observations confirment l’installation de cette espèce, découverte en Corse en 2012 (DELAGE, Candollea 67: 301, 2012). Si la plante est visiblement bien implantée et en progression en Casinca, où elle a été initialement observée, elle est en revanche nouvelle pour le Fium’Orbu et la région bastiaise.
description: Published as part of Jeanmonod, Daniel, 2015, Notes à la flore de Corse, XXV, pp. 109-140 in Candollea 70 (1) on pages 120-121, DOI: 10.15553/c2015v701a10, http://zenodo.org/record/5720895
Parectopa pselaphotis Meyrick 1915, n. sp.
Creators: name: Prins, Jurate De
name: Brito, Rosângela
name: Moreira, Gilson Rudinei Pires
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Lepidoptera
Gracillariidae
Parectopa
Parectopa pselaphotis

description: Parectopa pselaphotis Meyrick, 1915
" Parectopa pselaphotis, n. sp. "—Meyrick, E. 1915b. Transactions of the Entomological Society of London
(2): 237.
Type locality: Ecuador, Huigra, 4500 ft., vi.1914, leg. Parish. Type specimens: Holotype ♂, BMNH; 3 Paratypes ♂, ♀, BMNH. Distribution: Ecuador (Meyrick 1915b: 237).
Larval hostplant(s): Unknown.
description: Published as part of Prins, Jurate De, Brito, Rosângela & Moreira, Gilson Rudinei Pires, 2016, An annotated taxonomic checklist of the Neotropical Gracillariidae (Lepidoptera) with links to the information on host plants and parasitoids, pp. 1-51 in Zootaxa 4158 (1) on page 29, DOI: 10.11646/zootaxa.4158.1.1, http://zenodo.org/record/255480
Master plan of development, 1959
Creators: name: No Name Supplied
Publisher: name: No Publisher Supplied
Year: 1959

description: This 1959 report presents a master plan for the development of the borough of Haddonfield. NJ. It offers an analysis of those basic background elements to planning, and proposes objectives, directions and needs for the future. Topics covered include the population, land use, economy, municipal finances, traffic, business district, community facilities, housing, and zoning of Haddonfield.
Borough of Haledon master plan study, 1965
Creators: name: No Name Supplied
Publisher: name: No Publisher Supplied
Year: 1965

description: This report is presented in three parts to correspond with the three basic phases in the process of developing a master plan. Part I consists of a summary of the basic background elements essential to the planning process and for which detailed studies have previously been made. These background elements include land use and development, physical features, municipal economy and finance, traffic and circulation, community facilities and services and the like. Also included is a population study.  Part II is devoted to the Master Plan proposals. The Plan is presented in three parts; the Land Use Plan, the Street Plan and the Community Facilities Plan. Part III concerns itself with implementation of the Plan including a program for future capital improvements, continuing planning recommendations and other considerations which are important to the attainment of the Borough's long range objectives.
Assessing the role of lincRNAs in B-lymphoblastic leukemia
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: The role of a novel class of non-coding RNA, termed long intergenic non-coding RNA (lincRNA), has only begun to be explored in cancer. We have initiated an ambitious research study, using microarray-based profiling to characterize the expression of lincRNA in three cytogenetic subtypes of B-lymphoblastic leukemia (B-ALL). We have discovered a set of lincRNAs that are differentially regulated, out of which a set of seven lincRNAs demonstrate expression patterns that suggest roles as tumor suppressors and oncogenes. Here, we propose to functionally characterize one dysregulated lincRNA, BALIR-2, in oncogenesis and to develop it as a target for therapeutic intervention. BALIR-2 is expressed at high levels in pediatric B-ALL that show a worse prognosis and poor response to prednisone. We also recently made significant progress in defining the chromosomal location and gene structure of BALIR-2 in both human and mouse cells. To further understand BALIR-2 and its role in B-cell oncogenesis, we will examine whether gain-of-function can lead to oncogenesis in a mouse model and whether loss-of-function can hamper B-ALL cell growth. Furthermore, we will begin to define the cellular function of BALIR-2 by a combination of high-throughput and hypothesis-driven approaches. Together, these studies will lead to a better understanding of B-ALL and pave the way for new therapeutic interventions in this disease.
Flood control management, an overview of issues and responses
Creators: name: No Name Supplied
Publisher: name: No Publisher Supplied
Year: 1977

description: This report represents a summary of a two year research effort to identify and analyze the state's flood control responses and capabilities. It is clear that if flood hazards are to be reduced the existing approaches must be strengthened at all levels of government. This report includes suggested directions for improving the intergovernmental framework, organizational capacity and financial capability to achieve the desired objective. The report is divided into seven sections, reflecting the major concerns of the study. Section I offers a historical perspective of flooding in New Jersey and examines the causes for flooding and the steps that government has taken to minimize flood hazards. Section II briefly describes the various preventive and corrective approaches available to reduce flood risks. Sections Ill, IV, and V identify and discuss the activities of Federal, State, and substate governmental entities that are involved in flood management, including programs administered by the U.S. Army Corps of Engineers, the U.S. Department of Housing and Urban Development, the State Department of Environmental Protection, the State Soil Conservation Committee, county agencies, and municipal governments. Section VI consists of a summary of Statutory Authority and Section VII sets forth a number of recommendations to strengthen the intergovernmental response to flood control and establish a comprehensive system of flood management in New Jersey.
Rationally based combination immunotherapy: Active specific dendritic cell based vaccine (DC-Ad-GM-CAIX) and immune-checkpoint blockade targeting the PD1/PD-L1 pathway
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Carbonic anhydrase IX (CAIX) expression is constitutively up-regulated in clear cell renal cell carcinoma (ccRCC) due to loss of the VHL gene. Its up-regulation in ccRCC and low expression levels in normal tissues led us to develop a dendritic cell based vaccine (DC-Ad-GM·CAIX) targeting the CAIX tumor antigen. Using immunocompetent Balb/c mouse models we tested the efficacy and mechanism of the vaccine to prevent and treat the growth of a syngeneic RCC (RENCA) engineered to overexpress the human tumor associated antigen (TAA) carbonic anhydrase IX (NPR-IX). In both prevention and treatment models, the growth of syngeneic RCC tumors overexpressing CAIX (NPR-IX) was significantly inhibited. However, vaccine efficacy may be blunted by the immunosuppressive microenvironment characteristic of ccRCC patients, including the increased presence of regulatory T cells and upregulated inhibitory pathways such as the pathway of programmed cell death protein 1 (PD1) and its ligand CD274 (PDL1/ B7-H1). An investigator-initiated phase I clinical trial of DC-Ad-GM·CAIX monotherapy in patients with metastatic ccRCC has entered the clinic at UCLA. Herein, we propose to test in vivo the hypothesis that the therapeutic combination of a PD1 blocker (RMP1-14 antibody) given in conjunction with the DC-Ad-GM·CAIX vaccine will enhance the antitumor efficacy of the vaccine by altering the suppressive tumor microenvironment and increasing effector T cell viability, and activity without increasing its systemic toxicity. The translational goal of this application is the preclinical evaluation of this combination immunotherapy strategy with the potential to produce an improved clinical response to the DC-Ad-GM·CAIX vaccine. OBJECTIVES: to develop a novel immunotherapy combining a PD1 blocker (RMP1-14) with the DC-Ad-GM·CAIX vaccine with (1) improved efficacy relative to the monotherapies; (2) maintaining the drugs systemic toxicity/safety (3) and evaluate the mechanism(s) that enhanced efficacy. METHODS: Balb/c mice are transplanted s.c. with NPR-IX cells. After tumors are established, the mice are randomly allocated to 6 treatment groups that include DC-Ad-GM·CAIX immunotherapy, RMP1-14 antibody as monotherapies, combination of and controls. DCs will be ex-vivo differentiated from Balb/c bone marrow cells and then transduced to express the CAIX using the adenoviral vector Ad-GM·CAIX. Tumor growth and mice health will be monitored till the group with the fastest growing tumor reaches the maximal institutionally allowed tumor size. At
Hordeum marinum subsp. marinum
Creators: name: Jeanmonod, Daniel
Publisher: name: Zenodo
Year: 2015
Subjects: Biodiversity
Taxonomy
Plantae
Tracheophyta
Liliopsida
Poales
Poaceae
Hordeum
Hordeum marinum
Hordeum marinum huds. subsp. marinum

description: Hordeum marinum Huds. subsp. marinum
− Secteur Bonifacio, île de Cavallo, zone humide à hautes herbes, au S de l’étang de la Cala di u Grecu, un peu au N de l’aérodrome, près d’un héliport, 23.4.201 4, Delage, A. & Y. Petit s.n. (Hb. CBNC).
Nouvelle mention pour ce taxon considéré comme rare en Corse, où la subsp. gussoneanum (Parl.) Thell. est nettement plus fréquente. La plante avait déjà été autrefois récoltée à Bonifacio (sans précision), mais elle n’avait pas été mentionnée sur l’île de Cavallo jusqu’à présent à notre connaissance, l’observation initiale (inédite) datant toutefois de 1994 par G. Paradis & C. Lorenzoni.
description: Published as part of Jeanmonod, Daniel, 2015, Notes à la flore de Corse, XXV, pp. 109-140 in Candollea 70 (1) on page 121, DOI: 10.15553/c2015v701a10, http://zenodo.org/record/5720895
Master plan for Holmdel township, New Jersey
Creators: name: No Name Supplied
Publisher: name: No Publisher Supplied
Year: 1958

description: This 1958 report presents a master plan for the development of the borough of Holmdel, NJ. It offers an analysis of those basic background elements to planning, and proposes objectives, directions and needs for the future. Topics covered include a summary of existing conditions, population forecast, future land use, and public facilities of Holmdel.
Parectopa pulverella Walsingham 1897, sp. n.
Creators: name: Prins, Jurate De
name: Brito, Rosângela
name: Moreira, Gilson Rudinei Pires
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Lepidoptera
Gracillariidae
Parectopa
Parectopa pulverella

description: Parectopa pulverella (Walsingham, 1897)
" Gracilaria pulverella, sp. n. "—Walsingham, Lord (Thomas de Grey). 1897. Proceedings of the Zoological
Society of London 1897(10): 153.
Type locality: [Dominican Republic], San Domingo; [Virgin Islands], S[ain]t Thomas. Type specimens: Type ♀, coll. Hedemann, 1 more specimen (gender not stated) [not included in type series].
Note: The collection of Microlepidoptera of Wilhelm von Hedemann was obtained by A. Caradja via Hans Larsen. The specialized collection of Lepidoptera from Mexico is given to the Natural History Museum in Wien in 1865 (Horn & Kahle 1935: 107).
Distribution: Dominican Republic, Virgin Islands: Saint Thomas (Walsingham 1897: 153). Larval hostplant(s): Unknown.
description: Published as part of Prins, Jurate De, Brito, Rosângela & Moreira, Gilson Rudinei Pires, 2016, An annotated taxonomic checklist of the Neotropical Gracillariidae (Lepidoptera) with links to the information on host plants and parasitoids, pp. 1-51 in Zootaxa 4158 (1) on page 29, DOI: 10.11646/zootaxa.4158.1.1, http://zenodo.org/record/255480
Effects of the cessation of sewage sludge dumping at the 12-Mile Site, proceedings of the 12-Mile Dumpsite Symposium, Ocean Place Hilton Hotel, Long Branch, New Jersey, 18-19 June 1991
Creators: name: No Name Supplied
Publisher: name: No Publisher Supplied
Year: 1995

description: This report is based on the research from an innovative field study designed to evaluate effects on living marine resources and their habitats from sewage sludge dumping around the 12-Mile Dumpsite in the New York Bight that took place over several decades up to 1987. For three years, intensive field studies focused on three specific areas: 1) the replicate stations, NY6, R2, and NY11, which represented habitats that were respectively heavily degraded, enriched, and less severely influenced by sludge dumping; 2) the broadscale stations located throughout a 350 km2 area in the New York Bight apex, which provided a more descriptive background for the study; and 3) the Hudson Shelf Valley, in order to determine the fate of contaminants dispersed from the site. The major conclusions resulting from the study demonstrated that some indicators of sewage pollution in sediments, such as the rate of oxygen consumption by the seabed, redox potential, bottom dissolved-oxygen gradients, total bacteria count, and counts of coliform bacteria, responded rapidly to diminishing inputs of sewage sludge. The responses of the benthic community to the cessation of sludge dumping were not as apparent.
The development of novel mechanistically-based APE/Ref-1 redox inhibitors for human melanoma therapy
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: This proposal centers on the development of novel inhibitors of apurinic/apyrimidinic endonuclease (APE1), an important DNA excision repair enzyme, which also has a redox regulatory function known as Redox Factor-1 (Ref-1). It has been well documented that overexpression of APE/Ref-1 contributes to chemo-resistance and tumor progression in many human malignancies. APE/Ref-1 is therefore an attractive target for the development of novel and potent inhibitors. However, to the best of our knowledge, only limited success has been reported. The overall research strategy for this proposal is to identify potential candidates for highly specific APE/Ref-1 inhibitors based on our patented lead compounds using high throughput screening and structure-based optimization. In preliminary results to be outlined in this proposal we have found that SY-05 and SY598-21 exhibited promising APE/Ref-1 redox inhibitory effects and anti-tumor activity in vitro. Taking these candidates as lead compounds, we will develop more derivatives and further elucidate the relevance of APE/Ref-1 overexpression on redox regulation in vitro and in vivo. Specific Aim 1 – Structure Based Inhibitor Design and Optimization - A combination of virtual and high throughput methods will be used to identify drug candidates that target the redox function of APE/Ref-1 either directly or indirectly by interfering with APE/Ref-1 complexes. After initial screening by computational processes of docking and scoring, lead structures will be synthesized and their activity verified using an in vitro binding assay with purified APE/Ref-1. Top-ranked binders (lowest Kd) will be utilized for further redox activity analysis. Through extensive screening, structure-activity analysis and chemical optimization, we will be able to identify small molecular binders that effectively inhibit the redox activity of APE/Ref-1. Specific Aim 2 –To evaluate the in vivo pharmacologic activity of identified small molecules. After initial screening based on cell culture in vitro, promising candidate drugs derived from our lead compounds will be tested and evaluated in xenograft mouse models for their anti-tumor properties. The effects on tumor growth and metastases will be monitored and determined by Bioluminescence Tomography. To test the hypothesis that APE/Ref-1 inhibitors can reverse chemo-resistance, we will determine if these compounds synergistically enhance chemotherapy efficacy, both in vitro and in vivo. By the end of our studies we expect to develop 1-
Hydrodynamic model development for the Port of New York/New Jersey water level and current nowcast/forecast model system
Creators: name: No Name Supplied
Publisher: name: No Publisher Supplied
Year: 2001

description: The Port of NY/NJ water level and current nowcast/forecast model has been developed based on the three-dimensional barotropic Princeton Ocean Model. The purpose of developing this model is for water level and current predictions within the New York Harbor. The model will be the core component within the nowcast/forecast system which utilizes National Ocean Service Physical Oceanographic Real-Time System (NOS PORTS) information to provide near real-time nowcast information. With proper water level forecasts at Sandy Hook and Willets Point, wind stress forecasts such as National Weather Service’s  (NWS) Eta, and river flow forecasts, the system will also provide short term forecasts to mariners including ship pilots and the Coast Guard for optimizing ship operation, navigation safety, and vessel traffic control. The model includes a fine grid, which covers the Upper Bay, the Kill Van Kull, and Newark Bay, nested within the coarse grid. The model has been calibrated for tidal simulations and assessed with long term observed water levels and currents spanning over various time scales from tidal to seasonal cycles. In the near future the barotropic model will be extended to include the salinity and temperature.
Hyparrhenia hirta (L.) Stapf
Creators: name: Jeanmonod, Daniel
Publisher: name: Zenodo
Year: 2015
Subjects: Biodiversity
Taxonomy
Plantae
Tracheophyta
Liliopsida
Poales
Poaceae
Hyparrhenia
Hyparrhenia hirta

description: Hyparrhenia hirta (L.) Stapf subsp. hirta
− Secteur Incudine-Bavella, commune de Solaro, une dizaine de pieds sur le talus de la RN 198, au niveau du «Marais de Péri », 10 m, 13.6.200 3, Dardaine, P. s.n. (Hb. Privé).
Ce taxon est noté très rare (RR) dans Fl. Corsica (ed. 2, JEANMONOD & GAMISANS: 287, 2013) où il est indiqué d’une seule station située à Linguizetta. Les plantes portent une ligule courtement ciliée et de longs poils sur la bractée entourant l’ensemble de deux grappes.
description: Published as part of Jeanmonod, Daniel, 2015, Notes à la flore de Corse, XXV, pp. 109-140 in Candollea 70 (1) on page 121, DOI: 10.15553/c2015v701a10, http://zenodo.org/record/5720895
Workforce Implications of Large-scale Land-use Change in Aotearoa New Zealand
Creators: name: Bell, Kendon
name: Poehler, Elliot
name: Song, Zhongcen
name: Barker, Adam
Publisher: name: AgResearch
Year: 2024
Subjects: classificationCode: 3002
subject: Agriculture, land and farm management

description: Many stakeholders across the research sector, central and local government, and iwi Māori believe that diversifying land uses can help to achieve environmental and economic goals in Aotearoa New Zealand. This paper investigates how the workforce might constrain this process by developing a simulation model to examine the effect of land-use change on the labour market for regions in Aotearoa New Zealand. The model uses assumptions about workforce demand, population growth, the shortage reduction rate, and the labour supply elasticity to dynamically project changes in workforce size, wage rates, and workforce shortages in the food and fibre sector, at the regional scale. The 'Business as usual' scenario forecasts an average real wage growth of 12.7% and an average workforce expansion of 8.4% by the end of the simulation period in 2052. Additionally, it predicts an average peak regional workforce shortage of 8.3%. As a case study, we analyse the Northland region, simulating a regional push for horticulture developments beyond business-as-usual via investment in regional water storage projects. In our most expansive scenario, we simulate a real wage increase of 29.5%, a workforce increase of 43.2%, and a 14.9% peak shortage. In addition to the main scenario where the workforce increases due to higher wages, we analyse two alternative strategies to increase the workforce in response to this increased demand: growing the workforce via population growth and freeing up workers through converting pastoral land into forestry. Success for both strategies would require large deviations from status quo expectations. The results highlight that building a larger workforce will be a significant barrier to the ambition of labour-intensive land-use change, requiring a combination of strategies if land-use goals are to be met.
Parectopa quadristrigella Zeller 1877
Creators: name: Prins, Jurate De
name: Brito, Rosângela
name: Moreira, Gilson Rudinei Pires
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Lepidoptera
Gracillariidae
Parectopa
Parectopa quadristrigella

description: Parectopa quadristrigella (Zeller, 1877)
" G.[racilaria] quadristrigella "—Zeller, P.C. 1877. Horae Societatis Entomologicae Rossicae 13(1–4):
416–418; pl. 6, fig. 148.
Type locality: [Colombia], Bogotá.
Type specimens: Holotype ♂, BMNH.
Distribution: Colombia (Zeller 1877: 418).
Larval hostplant(s): Unknown.
description: Published as part of Prins, Jurate De, Brito, Rosângela & Moreira, Gilson Rudinei Pires, 2016, An annotated taxonomic checklist of the Neotropical Gracillariidae (Lepidoptera) with links to the information on host plants and parasitoids, pp. 1-51 in Zootaxa 4158 (1) on pages 29-30, DOI: 10.11646/zootaxa.4158.1.1, http://zenodo.org/record/255480
Synthesis of a unique macrocycle library targeting the mitotic regulator Pin1
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: This proposal concerns a unique synthetic approach to the preparation of macrocyclic peptidomimetics with potential pharmacological utility. The large ring structure of macrocycles is widely regarded as a ‘privileged’ structural motif in medicinal chemistry, but such compounds are scarcely present in chemical screening collections due to difficulty in their synthesis. We propose a novel means of preparing macrocycles which utilizes a latently reactive domain to guide the formation of large rings within short peptides. Accordingly, we term this reactive domain a macrocyclization ‘template’. The prototype template is attached to the N-terminus or a side chain amino group of a peptide, and initiates intramolecular Friedel-Crafts alkylation of electron rich amino acid side chains, including tyrosine and tryptophan. This reaction gives a mixture of isomeric macrocycles. While unconventional, synthesis of tractable mixtures is well suited for rapidly preparing diverse collections of molecules for biological screening. Additionally, our template is designed to impart drug like properties of metabolic stability, membrane permeability and target affinity. We hope to proceed directly from a primary amino acid sequence to a high affinity macrocyclic Pin1 inhibitor by starting from known peptide binding sequences gleaned from biochemical investigations. A solid-phase synthetic approach will facilitate rapid library construction of a targeted chemical library against the human mitotic regulatory enzyme Pin1. A functional enzyme assay against recombinant Pin1 will allow bioactivity-guided isolation of potent inhibitors. Demonstration of this novel method for targeted library synthesis has the potential to transform the way we approach drug discovery. Structurally complex macrocycles are well poised to modulate challenging therapeutic targets; particularly those involving protein-protein interaction as in Pin1. A potent, metabolically stable Pin1 inhibitor is essential for further investigation to validate Pin1 as a therapeutic target for cancer.
Parectopa refulgens Meyrick 1915, n. sp.
Creators: name: Prins, Jurate De
name: Brito, Rosângela
name: Moreira, Gilson Rudinei Pires
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Lepidoptera
Gracillariidae
Parectopa
Parectopa refulgens

description: Parectopa refulgens Meyrick, 1915
" Parectopa refulgens, n. sp. "—Meyrick, E. 1915b. Transactions of the Entomological Society of London
(2): 234.
Type locality: Ecuador, Huigra, 4500 ft., vi.1914, leg. Parisch. Type specimens: Holotype ♀, BMNH.
Distribution: Ecuador (Meyrick 1915b: 234).
Larval hostplant(s): Unknown.
description: Published as part of Prins, Jurate De, Brito, Rosângela & Moreira, Gilson Rudinei Pires, 2016, An annotated taxonomic checklist of the Neotropical Gracillariidae (Lepidoptera) with links to the information on host plants and parasitoids, pp. 1-51 in Zootaxa 4158 (1) on page 30, DOI: 10.11646/zootaxa.4158.1.1, http://zenodo.org/record/255480
Defining the role of a novel, developmentally restricted hematopoietic stem cell in pediatric leukemias
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: The goal of this proposal is to determine the role of a newly discovered putative pediatric cancer stem cell in infant leukemias. Leukemia is the most common childhood cancer, with B-cell acute lymphoblastic leukemia (B-ALL) representing over 90% of infant leukemias and the worst prognosis among pediatric cancers. Although there is clear evidence for a fetal origin of infant B-ALL, the inherent difficulty of studying human embryonic development and the lack of appropriate mouse models for this disease has limited our understanding of the cause and origin of B-ALL. Improved treatment strategies for this disease thus seem elusive. To delineate hematopoietic stem cell (HSC) differentiation pathways, we recently established a lineage-tracing mouse model that maps cells with a history of Flk2 expression. Analysis of hematopoiesis in this model revealed two coexisting populations of HSC during fetal development. Both populations are capable of long-term, multilineage repopulation of adult recipients upon primary and serial transplantation. Surprisingly, however, one HSC population does not persist into adulthood and is therefore restricted to fetal development. Importantly, this novel HSC population harbor functional and phenotypic similarities to cancer cells found in infant B-ALL. We hypothesize that this novel, developmentally-restricted HSC is the cell-of-origin in infant B-ALL. We propose to test this hypothesis by defining the molecular and functional properties of this novel cell population in vitro and in vivo, and after transformation with an oncogenic translocation gene frequently found in leukemic cells of children with B-ALL. Our long-term goal is to enable specific therapeutic targeting of cells causing pediatric leukemias.
Non-canonical Wnt signaling in glioblastoma invasiveness
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Glioblastoma multiforme (GBM) is among the most lethal of human cancers. The highly invasiveness nature of GBM cells allows them to evade surgical resection and give rise to distal secondary tumors within months of initial treatment. However, the mechanisms underlying GBM remain to be fully elucidated. We have very recently obtained evidence that the non-canonical Wnt signaling pathway plays a key role in regulating GBM cell motility in vitro. Our studies proposed here aim to determine whether this pathway similarly mediates GBM invasiveness in vivo. To begin to address this issue we will first create an orthotopic xenograft model of human GBM cell lines in athymic mice. GBM cells will be intracranially injected into mouse brains, and tumor growth followed over time by magnetic resonance imaging (MRI) to assess the rate of primary tumor growth as well as the development of secondary lesions. In addition, after euthanasia mouse brain tissue will be sectioned and analyzed histologically for the presence of primary and secondary tumors. Knockdown or overexpression of key components of non-canonical Wnt signaling in these cells will reveal the impact of this pathway on tumor growth rate and invasiveness. In addition, we will initiate the creation of a genetically engineered mouse model of GBM. In these studies, a retrovirus containing a PDGF-IRES-Cre cassette will be injected into the brains of mice dually homozygous for floxed p53 and PTEN to initiate tumors from endogenous neural precursor cells. Again, the rate of tumor initiation, primary tumor growth rate and the extent of invasiveness will be assessed by MRI, and histological examination of sections will be used to confirm the presence of secondary lesions. Addition of knockdown oligonucleotides targeting key components of the non-canonical Wnt pathway to the retrovirus will reveal the extent to which this pathway alters GBM invasiveness in this model. The successful completion of the proposed studies will begin to implicate the non-canonical Wnt signaling pathway in glioblastoma malignancy, hopefully opening up new avenues for therapeutic intervention. Moreover, these studies will provide preliminary data that will be necessary for the successful submission of a proposal for a more in-depth project to NIH in the near future.
Methods for genome-wide association with longitudinal phenotypes
Creators: name: Musolf, Anthony Mark
Publisher: name: No Publisher Supplied
Year: 2014

description: Most genome-wide association studies (GWAS) look for correlation between genetic variants and disease risk.  Correlation between variants and disease progression or severity is rare.  This maybe be due to the fact that progression GWAS require longitudinal data, which is much more difficult to analyze.  In this thesis, I present a new method for performing GWAS with longitudinal phenotypes.  Heterogeneous data is analyzed into homogeneous subgroups, and the probability of belonging to a given subgroup is used as a phenotype in association analyses.  Association analyses can be performed on single SNPs or regions of the genome for both family and population data sets.  Covariates can also be included in the analyses.  I report that this method maintains proper type I error under all genetic scenarios, including when admixture is present.  I also report that greater than 80% power is obtained for most genetic scenarios.  Thus, this method is suitable for use by researchers studying longitudinal diseases
Partition identities arising from the standard A(2)2-modules of level 4
Creators: name: Nandi, Debajyoti
Publisher: name: No Publisher Supplied
Year: 2014

description: In this dissertation, we propose a set of new partition identities, arising from a twisted vertex operator construction of the level 4 standard modules for the affine Kac-Moody algebra of type A(2)2 . These identities have an interesting new feature, absent from previously known examples of this type.    This work is a continuation of a long line of research of constructing standard modules for affine Kac-Moody algebras via vertex operators, and the associated combinatorial identities. The interplay between representation theory and combinatorial identities was exemplified by the vertex-algebraic proof of the famous Rogers-Ramanujan-type identities using standard A(1)1-modules by J. Lepowsky and R. Wilson. In his Ph.D.  thesis, S. Capparelli proposed new combinatorial identities using a twisted vertex operator construction of the standard A(2)2-modules of level 3, which were later proved independently by G. Andrews, S. Capparelli, and M. Tamba-C. Xie.    We begin with an obvious spanning set for each of the level 4 standard modules for A(2)2 , and reduce this spanning set using various relations. Most of these relations come from certain product generating function identities which are valid for all the level 4 modules. There are also other ad-hoc relations specific to a particular module of level 4. In this way, we reduce our spanning sets to match with the graded dimensions of the said modules as closely as possible. We conjecture and present strong evidence for three partition identities based on the spanning sets for the three standard A(2)2-modules of level 4.    One surprising result of our work is the discovery of relations of arbitrary length. Consequently, the partitions corresponding to these spanning sets cannot be described by difference conditions of finite length.    The spanning set result proves one inequality of the proposed identities. There is strong evidence for the validity of the conjecture (i.e., the opposite inequality), since it has been verified to hold for partitions of n ≤ 170, and n = 180, 190 and 200.
Structural and biochemical studies on regulation of microtubule dynamics through tubulin dimer biogenesis and activation
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Cell division is enabled by forces generated along microtubule (MT) cytoskeleton polymers. Cellular pathways precisely regulate how MTs are polymerized from soluble aß-tubulin dimers to align duplicated chromosomes, and are then depolymerized to segregate them to daughter cells. Defects in regulating MT assembly and disassembly dynamics lead to aneuploidy and chromosome missegregation, similar to those observed in pre-cancerous conditions. Complete inhibition of MT dynamics is an effective strategy to control rapidly dividing cancer cells using compounds such as Taxol or Vinblastine. MT polymerization is fundamentally enabled by a constant aß tubulin supply maintained within human cells. Strategies to control cell division by decreasing the tubulin supply can inhibit MT dynamics in cells, but remain out of reach due to a poor understanding of the cellular pathways maintain tubulin supply. In this proposal we aim to explore mechanisms of the tubulin cofactors that regulate tubulin dimer supply through biogenesis, activation and degradation. Each a and ß tubulin are catalytically assembled after folding separately to an aß-tubulin dimer by the tubulin cofactor pathway. Composed of six highly conserved proteins (five tubulin cofactors and a dedicated Arf-like G protein), this quality control pathway regulates the error-prone and non-spontaneous assembly of aß-tubulin. Despite decades of study, the tubulin cofactors remain poorly understood, in part due to a lack of biochemical reconstitution and biophysical studies. Since initiating my laboratory in 2011, we have made breakthroughs in reconstituting tubulin cofactors for biochemical and structural studies. We first aim to explore the biochemical steps in tubulin cofactor pathway to identify tubulin cofactor-tubulin assembly intermediates. We aim to study structures of the tubulin cofactors with tubulin using electron microscopy and x-ray crystallography. We will also reconstitute the purified tubulin cofactors with near physiological aß-tubulin concentration and dynamic MTs using single molecule total internal reflection fluorescence microscopy approaches. We intend to build additional data to apply for long term funding. Our long-term goal is to understand the physical mechanisms of tubulin cofactor and tubulin biogenesis to control tubulin dimer supply in cells. Decreasing the tubulin supply is a fundamental way to control MT and the studies described here can lead to developing new strategies to inhibit MT dynamics in rapidly dividing
Parectopa rotigera Meyrick 1931, n. sp.
Creators: name: Prins, Jurate De
name: Brito, Rosângela
name: Moreira, Gilson Rudinei Pires
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Lepidoptera
Gracillariidae
Parectopa
Parectopa rotigera

description: Parectopa rotigera Meyrick, 1931
" Parectopa rotigera n. sp. "—Meyrick, E. 1931a. Anales del Museo Nacional de Historia Natural de Buenos
Aires 36(): 400.
Type locality: S.[outh] Chile, Llanquihue Province, Casa Pangue. Type specimens: Holotype ♂, BMNH.
Distribution: Chile (Meyrick 1931a: 400).
Larval hostplant(s): Unknown.
description: Published as part of Prins, Jurate De, Brito, Rosângela & Moreira, Gilson Rudinei Pires, 2016, An annotated taxonomic checklist of the Neotropical Gracillariidae (Lepidoptera) with links to the information on host plants and parasitoids, pp. 1-51 in Zootaxa 4158 (1) on page 30, DOI: 10.11646/zootaxa.4158.1.1, http://zenodo.org/record/255480
Mechanism of hepatocarcinogenesis induced by hepatitis B virus
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Hepatocellular carcinoma (HCC) is one of the most lethal human cancers and is the third leading cause of cancer deaths worldwide. One major risk factor for HCC, affecting around 400 million people worldwide, is chronic hepatitis B virus (HBV) infection. The molecular mechanism through which chronic HBV infection contributes to hepatocarcinogenesis remains poorly understood. It has been showed recently that transgenic expression of the complete genome of HBV causes no chronic necroinflammation but significantly increases HCC incidence in three independent transgenic mouse lines, suggesting that HBV can contribute directly to the development of HCC. Employing this newly developed transgenic model of HBV hepatocarcinogenesis, we performed initial microarray analysis and identified a few HCC-associated genes whose expression appears to be altered in the non-tumor liver of HBV transgenic mice compared to the non-transgenic littermates. In the present proposal, we will focus on one of the genes, a fatty acid binding protein (Fabp). We will test the following two hypotheses: 1) transgenic expression of hepatitis B virus induces overexpression of the Fabp in mouse liver; 2) overexpression of the Fabp in liver cells can cause oncogenic transformation. The proposed study may find a novel mechanism important to HBV-induced HCC and identify a potential therapeutic target for liver cancer.
Novel Molecular Probes for Noninvasive Imaging
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: The advent of personalized medicine has increased the demand for new diagnostic and treatment monitoring approaches. Such approaches are especially needed in cancer therapies which are often based on drugs with low response rates and severe side effects. Recent studies show that positron emission tomography (typically called “PET”) can measure drug-induced changes in tumor activity. Such tumor ‘metabolic activity’ compares well with clinical endpoints and can be detected earlier than drug-induced changes in tumor size assessed by computed tomography (CT). These studies support a wider use of early PET probes and suggest that PET-based diagnostics could also be particularly useful to guide the selection of type of chemotherapy. Unfortunately, PET probes to guide the decision making process for chemotherapy are lacking so far. The availability of such probes could enable pre-therapeutic selection of patients and eliminate unnecessary treatments if tumors show low probe activity. We have discovered a novel PET probe that utilizes the same cellular mechanisms that an entire class of chemotherapeutic drugs, called anti-metabolics, act through to kill cancer cells. We propose to evaluate the potential of this probe to identify cancer cells that will respond to this common type of chemotherapy.
Ctenopelma nigrum Holmgren 1857
Creators: name: Sun, Shu-Ping
name: Sheng, Mao-Ling
name: Gao, Chun
Publisher: name: Zenodo
Year: 2019
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hymenoptera
Ichneumonidae
Ctenopelma
Ctenopelma nigrum

description: Ctenopelma nigrum Holmgren, 1857
Material examined
CHINA • 1 ♀; Beijing, Mentougou; 25 May 2009; reared from ‘cocoon’ (chamber) of Cephalcia lariciphila (Wachtl, 1898) by Tao Wang • 1 ♀, 10 ♂♂; Beijing, Mentougou; 25 May 2009; Tao Wang and Shi-Xiang Zong leg.; collected in forest of Larix principis -rupprechtii Mayr.
Host
Cephalcia lariciphila (Wachtl, 1898).
Host foodplant
Larix principis -rupprechtii Mayr.
Remarks
New record for China.
description: Published as part of Sun, Shu-Ping, Sheng, Mao-Ling & Gao, Chun, 2019, The species of Ctenopelma Holmgren (Hymenoptera, Ichneumonidae) from China, pp. 1-31 in European Journal of Taxonomy 545 on page 16, DOI: 10.5852/ejt.2019.545, http://zenodo.org/record/3382721
Hypothalamic DBS for the Treatment of Eating Disorders
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: This research project will develop a pig animal model (Gottingen minipig) to study molecular mechanisms associated with electrical stimulation, called deep brain stimulation (DBS). The DBS is provided by a small wire that is surgically positioned in a specific region of the brain called the dorsalmedial portion of the ventromedial hypothalamic nucleus (dmVMH). The dmVMH controls feeding and energy utilization and its modulation represents a novel method for the treatment of eating disorders and morbid obesity in particular. This animal model will be developed in collaboration with NeuroSigma, a venture-funded California start-up company, and it will complement a human feasibility study also supported by NeuroSigma that will target the same neural structure with DBS as a treatment for morbid obesity. The present study in a pig, a naturally obese animal, will establish a model that will allow us to study molecular mechanisms involved in the proposed DBS treatment. The outcome of this molecular research will increase our understanding of obesity that is associated with brain activity in the dmVMH DBS. Presently, the treatment of obesity is becoming an increasingly acute healthcare problem with significant impact on overall healthcare costs. In the US, obesity-related healthcare costs are estimated at approximately $100 billion annually, half of which is paid by Medicare and Medicaid. In California, according to the data for year 2000, these costs totaled $21.7 billion. This research will increase our knowledge of brain-associated mechansims of food intake and obesity that can be translated into novel therapies.