title: ÐÐ·ÑÑÐµÐ½Ð¸Ðµ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð¾Ð² ÐºÐ»ÑÐ±ÐµÐ½ÑÐºÐ¾Ð²ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ ÑÐ¾Ð´Ð° Sinorhizobium
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: The studying of bacteriophages of nodule bacteria of the genus Sinorhizobium
Creators: name: ÐÐ¶ÐµÐ³ÐµÑ, ÐÐµÑÐ¼Ð°Ð½
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð¸
Ð¿ÑÐ¾ÑÐ°Ð³Ð¸
ÐºÐ»ÑÐ±ÐµÐ½ÑÐºÐ¾Ð²ÑÐµ Ð±Ð°ÐºÑÐµÑÐ¸Ð¸
Ð»Ð¸ÑÐ¸ÑÐµÑÐºÐ°Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ
bacteriophages
prophages
nodule bacteria
lytic activity

description: Ð¦ÐµÐ»Ñ ÑÐ°Ð±Ð¾ÑÑ - Ð¸Ð·ÑÑÐ¸ÑÑ Ð¿Ð¾ÑÐ²ÐµÐ½Ð½ÑÐµ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð¸ Ð¸ Ð¿ÑÐ¾ÑÐ°Ð³Ð¸ ÐºÐ»ÑÐ±ÐµÐ½ÑÐºÐ¾Ð²ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ Ð²Ð¸Ð´Ð° Sinorhizobiummeliloti Ð´Ð»Ñ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ¸ Ð±Ð¸Ð¾ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð¿Ð¾Ð´ÑÐ¾Ð´Ð¾Ð² ÑÐ¾Ð·Ð´Ð°Ð½Ð¸Ñ ÑÐ°Ð³Ð¾ÑÑÑÐ¾Ð¹ÑÐ¸Ð²ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð² Ð´Ð»Ñ Ð±Ð¸Ð¾Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð². ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ Ð²ÑÐ¿Ð¾Ð»Ð½ÑÐ»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: ÐÑÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ ÑÑÐ°Ð¼Ð¼Ð¾Ð² Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð¾Ð² Ð¸ ÑÐ¸Ð·Ð¾Ð±Ð¸Ð¹ Ð¸Ð· Ð¾Ð±ÑÐ°Ð·ÑÐ¾Ð² Ð¿Ð¾ÑÐ², Ð¾ÑÐ¾Ð±ÑÐ°Ð½Ð½ÑÑ Ð¸Ð· Ð³ÐµÐ¾Ð³ÑÐ°ÑÐ¸ÑÐµÑÐºÐ¸ ÑÐ´Ð°Ð»ÐµÐ½Ð½ÑÑ ÑÐµÐ³Ð¸Ð¾Ð½Ð¾Ð²; ÐÑÐµÐ½ÐºÐ° ÑÐ¿ÐµÐºÑÑÐ° Ð»Ð¸ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÑÐ¸Ð·Ð¾Ð±Ð¸Ð¾ÑÐ°Ð³Ð¾Ð² Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ ÐºÐ¾Ð»Ð»ÐµÐºÑÐ¸Ð¸ ÑÑÐ°Ð¼Ð¼Ð¾Ð² ÐºÐ»ÑÐ±ÐµÐ½ÑÐºÐ¾Ð²ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ Ð³ÐµÐ½ÐµÑÐ¸ÐºÐ¸ Ð¸ ÑÐµÐ»ÐµÐºÑÐ¸Ð¸ Ð¼Ð¸ÐºÑÐ¾Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð¾Ð²; ÐÐ¾Ð¸ÑÐº Ð¸ Ð°Ð½Ð°Ð»Ð¸Ð· Ð¿ÑÐ¾ÑÐ°Ð³Ð¾Ð²ÑÑ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÐµÐ¹ Ð² Ð³ÐµÐ½Ð¾Ð¼Ð°Ñ ÑÑÐ°Ð¼Ð¼Ð¾Ð² S. meliloti; ÐÐ¾Ð»ÑÑÐµÐ½Ð¸Ðµ Ð¸ Ð°Ð½Ð°Ð»Ð¸Ð· ÑÐ°Ð³Ð¾ÑÐµÐ·Ð¸ÑÑÐµÐ½ÑÐ½ÑÑ Ð¼ÑÑÐ°Ð½ÑÐ¾Ð² S. meliloti. Ð Ð°Ð±Ð¾ÑÐ°Â Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð°Â Ð½Ð°Â Ð±Ð°Ð·ÐµÂ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸Â  Ð³ÐµÐ½ÐµÑÐ¸ÐºÐ¸ Ð¸ ÑÐµÐ»ÐµÐºÑÐ¸Ð¸ Ð¼Ð¸ÐºÑÐ¾Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð¾Ð² Ð¤ÐÐÐÐ£ ÐÐÐÐÐ¡Ð¥Ð. Ð Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¾ ÑÑÐ¸ Ð½Ð¾Ð²ÑÑ ÑÐ¸Ð·Ð¾Ð±Ð¸Ð¾ÑÐ°Ð³Ð° Ð¸ Ð¿ÑÐ¸ÑÐ¾Ð´Ð½ÑÐµ ÑÑÐ°Ð¼Ð¼Ñ ÐºÐ»ÑÐ±ÐµÐ½ÑÐºÐ¾Ð²ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹, ÑÐ°Ð·Ð»Ð¸ÑÐ°ÑÑÐ¸ÐµÑÑ Ð¿Ð¾ ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾ÑÑÐ¸ Ðº Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð°Ð¼. ÐÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ° Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð¾Ð² Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÂ­Ð·Ð¾Â­Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² (Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ñ Ð½ÐµÐ³Ð°ÑÐ¸Ð²Ð½ÑÑ ÐºÐ¾Ð»Ð¾Ð½Ð¸Ð¹, Ð¾ÑÐµÐ½ÐºÐ° Ð»Ð¸ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸, ÑÐ¸ÑÑ ÑÐ°Ð³Ð¾Ð²ÑÑ ÑÐ°ÑÑÐ¸Ñ) Ð¸ Ð¿ÑÐ¾ÑÐ°Ð³Ð¾Ð² (Ð¾ÑÐµÐ½ÐºÐ° Ð»Ð¾ÐºÐ°Ð»Ð¸Â­Ð·Ð°ÑÐ¸Ð¸ Ð² Ð³ÐµÐ½Ð¾Ð¼Ðµ, ÑÐ°Ð·Ð¼ÐµÑÐ°, Ð½Ð°Ð»Ð¸ÑÐ¸Ñ ÐºÐ¾Ð´Ð¸ÑÑÑÑÐ¸Ñ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÐµÐ¹) Ð² ÐºÐ¾Ð»Ð»ÐµÐºÑÐ¸Ð¾Ð½Ð½ÑÑ ÑÑÐ°Ð¼Ð¼Ð°Ñ S. meliloti. ÐÐ¾Ð»ÑÑÐµÐ½Ñ Ð¸ Ð¾ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸Ð·Ð¾Ð²Ð°Ð½Ñ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾-Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ð¼Ð¸ Ð¼ÐµÑÐ¾Ð´Ð°Ð¼Ð¸ ÑÐ°Ð³Ð¾ÑÐµÐ·Ð¸ÑÑÐµÐ½ÑÐ½ÑÐµ Ð¼ÑÑÐ°Ð½ÑÑ S. meliloti, ÐºÐ¾ÑÐ¾ÑÑÐµ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÑÑÑ Ð¸Ð½ÑÐµÑÐµÑ Ð´Ð»Ñ ÑÐ°Ð·Â­ÑÐ°Ð±Ð¾ÑÐºÐ¸ Ð±Ð¸Ð¾ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð¿Ð¾Ð´ÑÐ¾Ð´Ð¾Ð² ÑÐ¾Ð·Ð´Ð°Ð½Ð¸Ñ ÑÐ°Ð³Ð¾ÑÑÑÐ¾Ð¹ÑÐ¸Ð²ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð² Ð´Ð»Ñ Ð±Ð¸Ð¾Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð².
description: The purpose of the work is to study soil bacteriophages and prophages of nodule bacteria Sinorhizobium meliloti for the development of biotechnological approaches to the creation of phage-resistant strains for biological products. The tasks of the study: 1. Isolation of rhizobia and bacteriophage strains from soil samples taken from geographically remote regions; 2. Assessment of the degree of susceptibility of rhizobia strains to isolated and collectible strains of bacteriophages from the collection of the Laboratory of Genetics and Selection of Microorganisms; 3. Detection and analysis of prophage sequences in genomes of reference S. meliloti strains. 4. Obtaining and characterization of phage-resistant mutants of strain S. meliloti. The work was carried out on the basis of the Laboratory of Genetics and selection of microorganisms of All-Russia Research Institute for Agricultural Microbiology. In this work, three new rhizobiophages and native isolates of nodule bacteria were obtained, which are differed in resistance to bacteriophages. Characterization using biological methods was carried out for bacteriophages (morphology of negative colonies, assessment of lytic activity, titer of phage particles) and prophages (assessment of localization in the genome, size, presence of coding sequences) in S. meliloti collection strains. S. meliloti phage-resistant mutants were obtained and characterized by molecular approaches. Obtained data are promising for the production of biological products by biotechnological approaches.