title: ÐÐ·ÑÑÐµÐ½Ð¸Ðµ Ð¼ÐµÑ Ð°Ð½Ð¸Ð·Ð¼Ð° Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ð¼ÐµÐ¶Ð´Ñ Ð³ÐµÐ½Ð°Ð¼Ð¸ pms1 Ð¸ rad30 Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹ Saccharomyces cerevisiae
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: The mechanism of interaction between PMS1 and RAD30 genes of Saccharomyces cerevisiae yeast
Creators: name: ÐÐµÑÐ´ÑÑÐµÐ²Ð°, ÐÐ°ÑÐ¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÑÐµÐ¿Ð°ÑÐ°ÑÐ¸Ñ
ÑÑ-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¹ Ð¼ÑÑÐ°Ð³ÐµÐ½ÐµÐ·
Ð³Ð»Ð¾Ð±Ð°Ð»ÑÐ½ÑÐ¹ ÑÐµÐºÐ¿Ð¾Ð¹Ð½Ñ
Ð»Ð¾ÐºÐ°Ð»ÑÐ½ÑÐ¹ ÑÐµÐºÐ¿Ð¾Ð¹Ð½Ñ
repair
uv-induced mutagenesis
global checkpoint
local checkpoint

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ ÑÐ°ÑÐ°ÐºÑÐµÑÐ° Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ð³ÐµÐ½Ð¾Ð² PMS1 Ð¸ RAD30 Ð² ÐºÐ»ÐµÑÐºÐ°Ñ Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹ Saccharomyces cerevisiae Ð¿ÑÐ¸ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÐµ Ð£Ð¤-Ð»ÑÑÐ°Ð¼Ð¸. Ð ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¸ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ñ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð·Ð°Ð´Ð°ÑÐ¸: ÐÑÐ¾Ð°Ð½Ð°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°ÑÑ Ð½Ð°ÑÑÐ½ÑÐµ ÑÑÑÐ´Ñ Ð¿Ð¾ ÑÐµÐ¼Ðµ Ð¼ÑÑÐ°Ð³ÐµÐ½ÐµÐ·Ð° Ð¸ ÑÐµÐ¿Ð°ÑÐ°ÑÐ¸Ð¸ Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹ Saccharomyces cerevisiae; ÐÑÐµÐ½Ð¸ÑÑ Ð»ÐµÑÐ°Ð»ÑÐ½ÑÐ¹ ÑÑÑÐµÐºÑ Ð£Ð¤-Ð¾Ð±Ð»ÑÑÐµÐ½Ð¸Ñ Ð½Ð° ÑÑÐ°Ð¼Ð¼Ñ pms1Î, rad30Î Ð¸ rad30Îpms1Î Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹ Saccharomyces cerevisiae; ÐÑÐµÐ½Ð¸ÑÑ Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¹ Ð¼ÑÑÐ°Ð³ÐµÐ½ÐµÐ· Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹ Saccharomyces cerevisiae ÑÑÐ°Ð¼Ð¼Ð¾Ð² pms1Î, rad30Î Ð¸ rad30Îpms1Î, Ð²ÑÐ·Ð²Ð°Ð½Ð½ÑÐ¹ Ð£Ð¤-Ð¾Ð±Ð»ÑÑÐµÐ½Ð¸ÐµÐ¼. ÐÐ±ÑÐµÐºÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â ÐºÐ»ÐµÑÐºÐ¸ Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹ Saccharomyces cerevisiae ÑÑÐ°Ð¼Ð¼Ð¾Ð² pms1Î, rad30Î Ð¸ rad30Îpms1Î. Ð Ð°Ð±Ð¾ÑÐ° Ð±ÑÐ»Ð° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ ÐÐ°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ ÐÐµÐ½ÐµÑÐ¸ÐºÐ¸ Ð­ÑÐºÐ°ÑÐ¸Ð¾Ñ ÐÐÐ Ð ÐÐÐ¦ Â«ÐÑÑÑÐ°ÑÐ¾Ð²ÑÐºÐ¸Ð¹ Ð¸Ð½ÑÑÐ¸ÑÑÑ â ÐÐÐ¯Ð¤Â». Ð ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð³ÐµÐ½ RAD30 Ð°ÐºÑÐ¸Ð²Ð½Ð¾ Ð·Ð°Ð´ÐµÐ¹ÑÑÐ²Ð¾Ð²Ð°Ð½ Ð² Ð»Ð¾ÐºÐ°Ð»ÑÐ½Ð¾Ð¼ ÑÐµÐºÐ¿Ð¾Ð¹Ð½ÑÐµ; Ð±ÑÐ»Ð¸ Ð¿ÑÐµÐ´Ð»Ð¾Ð¶ÐµÐ½Ñ Ð´Ð²Ðµ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½ÑÐµ Ð¿ÑÐ¸ÑÐ¸Ð½Ñ Ð¿Ð¾Ð½Ð¸Ð¶ÐµÐ½Ð½Ð¾Ð¹ ÑÑÐ²ÑÑÐ²Ð¸ÑÐµÐ»ÑÐ½Ð¾ÑÑÐ¸ Ð´Ð²Ð¾Ð¹Ð½Ð¾Ð³Ð¾ Ð¼ÑÑÐ°Ð½ÑÐ° Ðº Ð£Ð¤-Ð¸Ð·Ð»ÑÑÐµÐ½Ð¸Ñ: ÑÐ¿Ð¸ÑÑÐ°ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð³ÐµÐ½Ð° RAD30 Ð³ÐµÐ½Ð¾Ð¼ PMS1 Ð¸Ð»Ð¸ Ð´Ð¸Ð¿Ð»Ð¾Ð¸Ð´Ð½Ð¾ÑÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹, Ð·Ð°Ð´ÐµÐ¹ÑÑÐ²Ð¾Ð²Ð°Ð½Ð½ÑÑ Ð² Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¸.
description: This work is devoted to determining the nature of the interaction of PMS1 and RAD30 genes in Saccharomyces cerevisiae yeast cells under UV treatment. During the study, the following tasks were completed: - To analyze scientific papers on the topic of mutagenesis and repair of yeast Saccharomyces cerevisiae; - To analyze the lethal effect of UV irradiation on strains pms1Î, rad30Î and rad30Îpms1Î of yeast Saccharomyces cerevisiae; - To analyze the induced mutagenesis of UV irradiation on strains pms1Î, rad30Î and rad30Îpms1Î of yeast Saccharomyces cerevisiae. The object of the study is Saccharomyces cerevisiae yeast cells of pms1Î, rad30Î and rad30Îpms1Î strains. The work was carried out based on the Eukaryote Genetics Laboratory of the PNPI named by B. P. Konstantinov of NRC Â«Kurchatov InstituteÂ».During the study, it was shown that the RAD30 gene is involved in the local checkpoint; two possible reasons for the reduced sensitivity of the double mutant to UV radiation were proposed: epistatization of the RAD30 gene by the PMS1 gene or diploidy of yeast cells involved in the investigation.