title: ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ ÑÐµÐºÑÐµÑÐ¾Ð¼Ð° Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð² Ð¿ÑÐ¾ÑÐµÑÑÐµ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐ¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Investigation of the secretome of human mesenchymal stem cells in the process of differentiation
Creators: name: ÐÐ¾Ð¿Ð¾Ð²Ð°, ÐÐºÐ°ÑÐµÑÐ¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÐµ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸
ÑÐµÐºÑÐµÑÐ¾Ð¼
Ð¿Ð°ÑÐ°ÐºÑÐ¸Ð½Ð½ÑÐµ ÑÑÑÐµÐºÑÑ
Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐ°
Ð¿ÑÐ¾ÑÐµÐ¾Ð¼
Regenerative medicine
mesenchymal stem cells
secretome
paracrine effects
differentiation
proteome

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description: The subject of the graduate qualification work is Â«Investigation of the secretome of human mesenchymal stem cells in the process of differentiationÂ». The given work is devoted to the investigation of changes in the composition of the human mesenchymal stem cells (MSCs) secretome. These changes are confirmed by detecting defferential protein expression in the cellsâ proteome at the stages of differentiation. To achieve the goal of investigation, methods of cultivating MSCs were used, the newest works in the field were studies and based on this a protocol for the collection of secretome with separation of fractions into soluble and vesicular was developed. To confirm the differentiation dyeing methods such as alkaline phosphatase and von Kossa were used with later visualization on the light microscope. For proteomic analysis, two-dimensional differential electrophoresis and liquid chromatography mass spectrometric (LC-MS) analysis were used. The result was the identification of differential proteins expressions between the control and differentiated cells and proteinsâ functional annotation, which makes it possible to define the processes that are more active in cells during the differentiation process. Analysis of the results made it possible to better understand the staging of this process and to determine its molecular markers. The application area of the results is fundamental science and regenerative medicine with using MSCs secretome. â