title: Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´ÑÑÐ² ÑÐµÑ Ð½Ð¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¶Ð¸Ð´ÐºÐ¾Ð³Ð¾ Ð±Ð¸Ð¾ÑÑÐ½Ð³Ð¸ÑÐ¸Ð´Ð° Trichoderma viride Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð³Ð»ÑÐ±Ð¸Ð½Ð½Ð¾Ð³Ð¾ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Development of a liquid biofungicide Trichoderma viride technology by submerged cultivation method
Creators: name: Ð ÑÐ¼ÑÐ½ÑÐµÐ²Ð°, ÐÐ»ÑÐ³Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: trichoderma viride
Ð±Ð¸Ð¾ÑÑÐ½Ð³Ð¸ÑÐ¸Ð´
Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½Ð°Ñ ÑÑÐµÐ´Ð°
Ð³Ð»ÑÐ±Ð¸Ð½Ð½Ð¾Ðµ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
ÑÑÐ½Ð³Ð¸ÑÐ¸Ð´Ð½Ð°Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ
biofungicide
nutrient medium
submerged cultivation
fungicidal activity

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐµ ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¶Ð¸Ð´ÐºÐ¾Ð³Ð¾ Ð±Ð¸Ð¾ÑÑÐ½Ð³Ð¸ÑÐ¸Ð´Ð° Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ Ð³ÑÐ¸Ð±Ð° Trichoderma viride 6V Ð½Ð° ÑÐ¾Ð½Ðµ ÑÐ¾ÑÑÐ°Ð½ÐµÐ½Ð¸Ñ Ð²ÑÑÐ¾ÐºÐ¸Ñ ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ ÑÐ²Ð¾Ð¹ÑÑÐ² Ð¸ ÑÑÐ½Ð³Ð¸ÑÐ¸Ð´Ð½Ð¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÐºÑÐ»ÑÑÑÑÑ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð³Ð»ÑÐ±Ð¸Ð½Ð½Ð¾Ð³Ð¾ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ. ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: â ÑÐ¾ÑÑÐ°Ð²Ð¸ÑÑ Ð¸ Ð¸ÑÐ¿ÑÑÐ°ÑÑ ÑÑÐ´ ÑÐµÑÐµÐ¿ÑÑÑ Ð¿Ð¾Ð»ÑÑÐ¸Ð½ÑÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½ÑÑ ÑÑÐµÐ´ Ð´Ð»Ñ Ð²ÑÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ñ T. viride 6V Ð² ÐºÐ¾Ð»Ð±Ð°Ñ; â Ð¾ÑÐ¾Ð±ÑÐ°ÑÑ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ Ð¿ÑÐ¾Ð´ÑÐºÑÐ¸Ð²Ð½ÑÑ Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½ÑÑ ÑÑÐµÐ´Ñ Ð¿Ð¾ÑÑÐµÐ´ÑÑÐ²Ð¾Ð¼ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ ÑÐ¸ÑÑÐ¾Ð² ÐºÑÐ»ÑÑÑÑ, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð¿ÑÐ¸Ð·Ð½Ð°ÐºÐ¾Ð²; â Ð¿Ð¾Ð´Ð¾Ð±ÑÐ°ÑÑ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÑ Ð³Ð»ÑÐ±Ð¸Ð½Ð½Ð¾Ð³Ð¾ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÑÐµÐ»ÐµÐ²Ð¾Ð³Ð¾ Ð¼Ð¸ÐºÑÐ¾Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð° Ð² Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ½Ð¾Ð¼ ÑÐµÑÐ¼ÐµÐ½ÑÐµÑÐµ; â Ð¾ÑÐµÐ½Ð¸ÑÑ ÑÑÐ½Ð³Ð¸ÑÐ¸Ð´Ð½ÑÑ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð¼Ð¸ÐºÑÐ¾Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð° Ð¿Ð¾ÑÐ»Ðµ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ; â Ð¿Ð¾Ð»ÑÑÐ¸ÑÑ Ð¾Ð¿ÑÑÐ½ÑÑ Ð¿Ð°ÑÑÐ¸Ñ Ð¶Ð¸Ð´ÐºÐ¾Ð³Ð¾ Ð±Ð¸Ð¾ÑÑÐ½Ð³Ð¸ÑÐ¸Ð´Ð° Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ T. viride 6V Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð³Ð»ÑÐ±Ð¸Ð½Ð½Ð¾Ð³Ð¾ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð² Ð¿ÑÐ¾Ð¼ÑÑÐ»ÐµÐ½Ð½Ð¾Ð¼ ÑÐµÑÐ¼ÐµÐ½ÑÐµÑÐµ. ÐÐ±ÑÐµÐºÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â Ð³ÑÐ¸Ð± Trichoderma viride ÑÑÐ°Ð¼Ð¼ 6V Ð¸Ð· ÐÐµÐ´Ð¾Ð¼ÑÑÐ²ÐµÐ½Ð½Ð¾Ð¹ ÐºÐ¾Ð»Ð»ÐµÐºÑÐ¸Ð¸ Ð¼Ð¸ÐºÑÐ¾Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð¾Ð² ÑÐµÐ»ÑÑÐºÐ¾ÑÐ¾Ð·ÑÐ¹ÑÑÐ²ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð½Ð°Ð·Ð½Ð°ÑÐµÐ½Ð¸Ñ Ð¤ÐÐÐÐ£ ÐÐÐÐÐ¡Ð¥Ð. ÐÑÐ¸ Ð½Ð°Ð¿Ð¸ÑÐ°Ð½Ð¸Ð¸ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±Ð·Ð¾ÑÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ: eLIBRARY.RU, Ð¡yberLeninka, NCBI. ÐÑÐ½Ð¾Ð²Ð½Ð°Ñ ÑÐ°ÑÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ Ð¤ÐÐÐÐ£ ÐÐÐÐÐ¡Ð¥Ð. ÐÐ¿ÑÑÐ½Ð°Ñ Ð¿Ð°ÑÑÐ¸Ñ Ð¶Ð¸Ð´ÐºÐ¾Ð³Ð¾ Ð±Ð¸Ð¾ÑÑÐ½Ð³Ð¸ÑÐ¸Ð´Ð° Ð¿Ð¾Ð»ÑÑÐµÐ½Ð° Ð½Ð° Ð±Ð¸Ð¾ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð¼ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´ÑÑÐ²Ðµ Â«Ð­ÐºÐ¾Ñ ÐÐ¸Ð¾Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÑÂ».Â  Ð ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐ¾ÑÑÐ°Ð²Ð»ÐµÐ½Ð° Ð¸ Ð¸ÑÐ¿ÑÑÐ°Ð½Ð° Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½Ð°Ñ ÑÑÐµÐ´Ð° Ñ Ð²ÑÑÐ¾ÐºÐ¾Ð¹ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸ÐµÐ¹ Ð³Ð»ÑÐºÐ¾Ð·Ñ Ð² ÑÐ¾ÑÑÐ°Ð²Ðµ, Ð¾Ð±ÐµÑÐ¿ÐµÑÐ¸Ð²Ð°ÑÑÐ°Ñ Ð²ÑÑÐ¾ÐºÐ¸Ð¹ ÑÐ¸ÑÑ ÑÐµÐ»ÐµÐ²Ð¾Ð³Ð¾ Ð¼Ð¸ÐºÑÐ¾Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð° Ð¸ ÐµÐ³Ð¾ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ Ð¿Ð¾Ð»Ð½ÑÐ¹ Ð¿ÐµÑÐµÑÐ¾Ð´ Ð² ÑÐ¾ÑÐ¼Ñ ÐºÐ¾Ð½Ð¸Ð´Ð¸Ð¹. ÐÑÐ¾Ð¸Ð·Ð²ÐµÐ´ÐµÐ½ Ð¿ÑÐ¾ÑÐµÑÑ Ð³Ð»ÑÐ±Ð¸Ð½Ð½Ð¾Ð³Ð¾ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð³ÑÐ¸Ð±Ð° Ð½Ð° Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼Ð¾Ð¹ Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ´Ðµ Ð² Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ½Ð¾Ð¼ ÑÐµÑÐ¼ÐµÐ½ÑÐµÑÐµ Ð² ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ð¸ ÑÐ¾ ÑÑÐ°Ð½Ð´Ð°ÑÑÐ½Ð¾Ð¹ Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ´Ð¾Ð¹. ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð° ÑÑÐ½Ð³Ð¸ÑÐ¸Ð´Ð½Ð°Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÐºÑÐ»ÑÑÑÑÑ. ÐÑÐ±ÑÐ°Ð½Ð½ÑÐµ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÑ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ Ð´Ð»Ñ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ Ð¾Ð¿ÑÑÐ½Ð¾Ð¹ Ð¿Ð°ÑÑÐ¸Ð¸ Ð¶Ð¸Ð´ÐºÐ¾Ð³Ð¾ Ð±Ð¸Ð¾ÑÑÐ½Ð³Ð¸ÑÐ¸Ð´Ð° T. viride 6V Ð² Ð¿ÑÐ¾Ð¼ÑÑÐ»ÐµÐ½Ð½Ð¾Ð¼ ÑÐµÑÐ¼ÐµÐ½ÑÐµÑÐµ Ð½Ð° Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼Ð¾Ð¹ Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ´Ðµ. Ð¡ÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÑÑ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»Ð¸ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿Ð°ÐºÐµÑÐ° Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼ Microsoft Excel.
description: This work is devoted to the development of a liquid biofungicide Trichoderma viride technology while maintaining high technological properties and fungicidal activity of the culture by submerged cultivation. The tasks of the study: â to formulate and test several formulations of semi-synthetic nutrient medium for the cultivation of T. viride 6V in flasks; â to select the most productive nutrient medium by determining culture titres and studying morphological characteristics; â to choose parameters of submerged cultivation of the target microorganism in a laboratory fermenter; â to evaluate the fungicidal activity of the microorganism after cultivation; â to obtain a test batch of liquid biofungicide T. viride 6V by submerged cultivation in an industrial fermenter. The object of the study is Trichoderma viride 6V fungi from the Departmental collection of agricultural microorganisms of All-Russian Research Institute of Agricultural Microbiology. The following databases were used to write the literature review: eLIBRARY.RU, CyberLeninka, NCBI. The main part of the research was carried out on the basis of All-Russian Research Institute of Agricultural Microbiology. A test batch of liquid biofungicide was obtained at the biotechnological company "Ecos Biopreparations". During the research, a nutrient medium with a high concentration of glucose in its composition, providing a high titer of the target microorganism and its most complete transition to the conidia form, was formulated, and tested. The process of fungi submerged cultivation on the nutrient medium under study in a laboratory fermenter was carried out in comparison with the standard nutrient medium. The fungicidal activity of the microbial culture was studied. The selected cultivation parameters were used to produce a test batch of T. viride 6V liquid biofungicide in a commercial fermenter on the nutrient medium under study. Statistical processing of the experimental data was carried out using Microsoft Excel.