Additional file 1 of Myocardial effects of angiotensin II compared to norepinephrine in an animal model of septic shock
Creators: name: Garcia, Bruno
name: Su, Fuhong
name: Dewachter, Laurence
name: Favory, Raphaël
name: Khaldi, Amina
name: Moiroux-Sahraoui, Alexander
name: Annoni, Filippo
name: Vasques-Nóvoa, Francisco
name: Rocha-Oliveira, Estela
name: Roncon-Albuquerque, Roberto
name: Hubesch, Geraldine
name: Njimi, Hassane
name: Vincent, Jean-Louis
name: Taccone, Fabio S.
name: Creteur, Jacques
name: Herpain, Antoine
Publisher: name: figshare
Year: 2022
Subjects: subject: Biophysics
subject: Medicine
subject: Physiology
subject: FOS: Biological sciences
subject: Pharmacology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences

description: Additional file 1. Figure S1. Pressure-volume loop illustration. Table S1. Primers used for real-time quantitative polymerase chain reaction (RTQ-PCR) in porcine myocardial tissue. Table S2. Hemodynamic variables in the three groups at the different study time-points. *p-value &lt;0.05 between NE and Ang II. †p-value &lt; 0.05 between NE and Sham. ‡p-value &lt; 0.05 between Ang II and Sham. P-value &lt; 0.05 compared to baseline for NE (§), Ang II (ll) and Sham (**) groups. HR: heart rate; MAP: mean arterial pressure; SV: stroke volume; CO: cardiac output; RAP: right atrial pressure; LVEDV: left ventricular end diastolic volume; LVESV: left ventricular end systolic volume; LVEDP: left ventricularend diastolic pressure; EF: ejection fraction; PRSW: preload recruitable stroke work; Emax: left ventricular maximal elastance; Ea: effective arterial elastance; Ea/Emax: left ventriculo-arterial coupling; V30: LV volume at 30 mmHg on the End Diastolic Pressure Volume Relationship; V100: LV volume at 100 mmHg on the End Systolic Pressure Volume Relationship; NE: norepinephrine; Ang: angiotensin PV loop analysis was obtained at baseline, fluids, vasopressor 1 and vasopressor 2. Table S3. Biological and oxygenation values in the three groups at the different study timepoints. *p-value &lt;0.05 between NE and Ang II. †p-value &lt; 0.05 between NE and Sham. ‡p-value &lt; 0.05 between Ang II and Sham. p-value &lt; 0.05 compared to baseline for NE (§), Ang II (ll) and Sham (**) groups. CO2 gap: veno-arterial difference in CO2 partial pressure; SVO2: mixed venous oxygen saturation; BE: base excess; IL: interleukin; TNF: tumor necrosis factor; NE: norepinephrine; Ang: angiotensin. Table S4. Respiratory variables. Results are presented as mean + SD. *p-value between NE and Ang II groups. †p-value &lt; 0.05 between NE and Sham groups. ‡p-value &lt; 0.05 between Ang II and Sham groups. p-value &lt; 0.05 compared to baseline for NE (§), Ang II (ll) and Sham (**) groups. PaO2: Arterial partial pressure of oxygen; FiO2 fraction of oxygen inspired; Pplat: plateau pressure; Crs:compliance of the respiratory system; EtCO2: end-tidal carbon dioxide; PaCO2: arterial partial pressure of carbon dioxide. Table S5. Blood gas analysis. Values are presented as mean + SD. *p-value between NE and angiotensin II group. †p-value &lt; 0.05 between NE and Sham group. ‡p-value &lt; 0.05 between angiotensin II and Sham group. P-value &lt; 0.05 compared to baseline for NE (§), angiotensin II (ll) and Sham (**) group. Hb: hemoglobin; Ht: hematocrit. PaO2: arterial partial pressure of oxygen. PaCO2: arterial partial pressure of carbon dioxide. Table S6. Biological variables. Values are expressed as mean ± SD. *p-value between NE and Ang II groups. †p-value &lt; 0.05 between NE and Sham groups. ‡p-value &lt; 0.05 between Ang II and Sham groups. p-value &lt; 0.05 compared to baseline for NE (§), Ang II (ll) and Sham (**) groups. ASAT: Aspartate aminotransferase; ALAT Alanine aminotransferase; LDH: Lactate dehydrogenase. Figure S2. A. Left ventricular mRNA expression of molecules implicated in Ca2+ handling and contractile apparatus [ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 (SERCA2A) and phospholamban (PLB)] B. LV mRNA expression of the ratio Bax/Bcl2 in norepinephrine (black bars) and angiotensin II groups. *pvalue&lt; 0.05. C. Cardiac apoptotic rate: ratio of apoptotic nuclei (TUNEL-positive or brown nuclei) to total nuclei (brown+blue nuclei) (x100 to be expressed as a percentage). D. mRNA expression of cell adhesion molecules (ICAM1, 2 and VCAM1) and eNOS, iNOS, nNOS in norepinephrine and angiotensin II groups compared to sham group. Figure S3. Fold Changes expressed in % between baseline and vasopressor 2 time points