title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ ÑÐ°Ð´Ð¸Ð°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð³Ð¾ Ð¸Ð·Ð»ÑÑÐµÐ½Ð¸Ñ Ð½Ð° Ð²ÑÐ¶Ð¸Ð²Ð°ÐµÐ¼Ð¾ÑÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð·Ð»Ð¾ÐºÐ°ÑÐµÑÑÐ²ÐµÐ½Ð½ÑÑ Ð³Ð»Ð¸Ð¾Ð¼ Ð¸ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð³ÐµÐ½Ð¾Ð² GLI1, TP73 Ð¸ RAD51
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: The effect of radiation exposure on the survival of malignant glioma cells and the expression of the GLI1, TP73 and RAD51 genes
Creators: name: Ð¡Ð¾Ð»ÑÐ½Ð¸Ðº, ÐÐ½Ð°ÑÑÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð³Ð»Ð¸Ð¾Ð¼Ñ
Ð»ÑÑÐµÐ²Ð°Ñ ÑÐµÑÐ°Ð¿Ð¸Ñ
Sonic Hedgehog
GLI1
RAD51
TP73
GANT61
ÑÐ¸ÐºÐ»Ð¾Ð¿Ð°Ð¼Ð¸Ð½
glioma
radiation therapy
cyclopamine

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾-Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸Ðº Ð³Ð»Ð¸Ð¾Ð±Ð»Ð°ÑÑÐ¾Ð¼ Ð¸ Ð¾ÑÐµÐ½ÐºÐµ Ð¸Ñ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð½Ð° Ð²ÑÐ¶Ð¸Ð²Ð°ÐµÐ¼Ð¾ÑÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð¿ÑÐ¸ Ð²Ð¾Ð·-Ð´ÐµÐ¹ÑÑÐ²Ð¸Ð¸ Ð¸Ð·Ð»ÑÑÐµÐ½Ð¸Ñ Ð¸ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾ÑÐ¾Ð² ÑÐ¸Ð³Ð½Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð¿ÑÑÐ¸ Sonic Hedgehog. ÐÐ»Ð¸Ð¾Ð±Ð»Ð°ÑÑÐ¾Ð¼Ñ ÑÐ²Ð»ÑÑÑÑÑ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ Ð°Ð³ÑÐµÑÑÐ¸Ð²Ð½ÑÐ¼Ð¸ Ð¾Ð¿ÑÑÐ¾Ð»ÑÐ¼Ð¸ Ð³Ð¾Ð»Ð¾Ð²Ð½Ð¾Ð³Ð¾ Ð¼Ð¾Ð·Ð³Ð°, ÐºÐ»ÐµÑÐºÐ¸ ÐºÐ¾ÑÐ¾ÑÑÑ Ð¾ÑÐ»Ð¸ÑÐ°ÑÑÑÑ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ð¼Ð¸ Ð¸ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾-Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ð¼Ð¸ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ°Ð¼Ð¸, Ð¿Ð¾ÑÑÐ¾Ð¼Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð½Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð»Ð¸Ð½Ð¸Ð¹ Ð³Ð»Ð¸Ð¾Ð±Ð»Ð°ÑÑÐ¾Ð¼ Ð¿Ð¾Ð·Ð²Ð¾Ð»Ð¸Ñ Ð¿Ð¾Ð²ÑÑÐ¸ÑÑ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÑÑÑÐµÑÑÐ²ÑÑÑÐ¸Ñ ÑÑÐµÐ¼ ÑÐµÑÐ°Ð¿Ð¸Ð¸. ÐÐ»Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¸ Ð²ÑÐ±ÑÐ°Ð½Ñ ÑÑÐ¸ Ð³ÐµÐ½Ð°, ÑÑÐ¾Ð²ÐµÐ½Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ ÐºÐ¾ÑÐ¾ÑÑÑ Ð¼Ð¾Ð¶ÐµÑ ÑÐºÐ°Ð·ÑÐ²Ð°ÑÑÑÑ Ð½Ð° Ð¾ÑÐ²ÐµÑÐµ ÐºÐ»ÐµÑÐ¾Ðº Ð½Ð° ÑÐµÑÐ°Ð¿Ð¸Ñ. ÐÐ° Ð´Ð°Ð½Ð½ÑÐ¹ Ð¼Ð¾Ð¼ÐµÐ½Ñ ÑÑÑÐµÑÑÐ²ÑÐµÑ Ð¼Ð½Ð¾Ð¶ÐµÑÑÐ²Ð¾ ÑÐ°Ð±Ð¾Ñ, Ð¿Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÑÑÐ¸Ñ ÑÑÐ°ÑÑÐ¸Ðµ Ð¿ÑÑÐ¸ Sonic Hedgehog Ð² ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ð¸ ÑÐ°ÐºÐ°. ÐÐ¾ÑÑÐ¾Ð¼Ñ Ð² ÑÐ°Ð±Ð¾ÑÐµ Ð±ÑÐ»Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð° ÑÐ¸ÑÐ¾ÑÐ¾ÐºÑÐ¸ÑÐ½Ð¾ÑÑÑ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾ÑÐ¾Ð² ÑÑÐ¾Ð³Ð¾ ÑÐ¸Ð³Ð½Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð¿ÑÑÐ¸ - ÑÐ¸ÐºÐ»Ð¾Ð¿Ð°Ð¼Ð¸Ð½Ð° Ð¸ GANT61, Ð° ÑÐ°ÐºÐ¶Ðµ, Ð¾ÑÐµÐ½ÐµÐ½ ÑÐ°Ð´Ð¸Ð¾ÑÐµÐ½ÑÐ¸Ð±Ð¸Ð»Ð¸Ð·Ð¸ÑÑÑÑÐ¸Ð¹ ÑÑÑÐµÐºÑ ÑÐ¸ÐºÐ»Ð¾Ð¿Ð°Ð¼Ð¸Ð½Ð°. ÐÐ¿ÑÑÐ¾Ð»ÐµÐ²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸ Ð¾Ð±Ð»ÑÑÐ°Ð»Ð¸ Ð³Ð°Ð¼Ð¼Ð°- Ð¸ ÑÐµÐ½ÑÐ³ÐµÐ½Ð¾Ð²ÑÐºÐ¸Ð¼Ð¸ Ð»ÑÑÐ°Ð¼Ð¸ Ð´Ð¾Ð·Ð°Ð¼Ð¸ 1-6 ÐÑ. Ð§ÑÐ²ÑÑÐ²Ð¸ÑÐµÐ»ÑÐ½Ð¾ÑÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ðº Ð¾Ð±Ð»ÑÑÐµÐ½Ð¸Ñ Ð¸ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾ÑÐ°Ð¼ Ð¾ÑÐµÐ½Ð¸Ð²Ð°Ð»Ð¸ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÑÐµÐ·Ð°Ð·ÑÑÐ¸Ð½-ÑÐµÑÑÐ° Ð¸ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° ÐºÐ¾Ð»Ð¾Ð½Ð¸Ð¹. Ð­ÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð³ÐµÐ½Ð¾Ð² Ð¾ÑÐµÐ½Ð¸Ð²Ð°Ð»Ð¸ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÐÐ¢-ÐÐ¦Ð . Ð Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ Ð¼Ñ Ð¾ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸Ð·Ð¾Ð²Ð°Ð»Ð¸ Ð¾ÑÐ²ÐµÑ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð»Ð¸Ð½Ð¸Ð¹ Ð·Ð»Ð¾ÐºÐ°ÑÐµÑÑÐ²ÐµÐ½Ð½ÑÑ Ð³Ð»Ð¸Ð¾Ð¼ Ð½Ð° ÑÐ°Ð´Ð¸Ð¾ÑÐµÑÐ°Ð¿Ð¸Ñ Ð¸ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾ÑÐ¾Ð², Ð¾ÑÐµÐ½Ð¸Ð»Ð¸ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ ÑÑÐ¾Ð²Ð½ÐµÐ¹ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Ð¿ÑÐ¸ Ð²Ð¾Ð·Ð´ÐµÐ¹ÑÑÐ²Ð¸Ð¸ Ð¸Ð·Ð»ÑÑÐµÐ½Ð¸Ñ. GANT61 Ð¾ÐºÐ°Ð·Ð°Ð» Ð¼ÐµÐ½ÐµÐµ ÑÐ¾ÐºÑÐ¸ÑÐ½ÑÐ¹ ÑÑÑÐµÐºÑ Ð½Ð° Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼ÑÐµ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐµ Ð»Ð¸Ð½Ð¸Ð¸ Ð¿Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ Ñ ÑÐ¸ÐºÐ»Ð¾Ð¿Ð°Ð¼Ð¸Ð½Ð¾Ð¼. ÐÐ»Ñ Ð½ÐµÐºÐ¾ÑÐ¾ÑÑÑ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ ÐºÑÐ»ÑÑÑÑ ÑÐ¸ÐºÐ»Ð¾Ð¿Ð°Ð¼Ð¸Ð½, Ð´Ð¾Ð±Ð°Ð²Ð»ÐµÐ½Ð½ÑÐ¹ Ð´Ð¾ Ð¾Ð±Ð»ÑÑÐµÐ½Ð¸Ñ, ÑÐ¿Ð¾ÑÐ¾Ð±ÐµÐ½ ÑÑÐ¸Ð»Ð¸Ð²Ð°ÑÑ ÑÑÑÐµÐºÑ Ð¾Ñ Ð¾Ð±Ð»ÑÑÐµÐ½Ð¸Ñ.
description: This work is devoted to the study of the genetic characteristics of glioblastomas and the assessment of their effect on cell survival under the influence of radiation and inhibitors of the Sonic Hedgehog signaling pathway. Glioblastomas are the most aggressive brain tumors, whose cells differ in genetic characteristics, so the study of new glioblastoma cell lines will increase the effectiveness of existing therapy regimens. Three genes were selected for the study, the expression level of which may affect the response of cells to therapy. At the moment, there are many works showing the involvement of the Sonic Hedgehog pathway in the development of cancer. Therefore, the cytotoxicity of cyclopamine and GANT61 was investigated. The radiosensitizing effect of cyclopamine was also evaluated. Tumor cells were irradiated with gamma and X-rays in doses of 1-6 Gy. The sensitivity of cells to radiation and inhibitors was assessed using a resazurin test and colony analysis. Gene expression was evaluated using RT-PCR. In this work, we characterized the response of malignant glioma cell lines to radiotherapy and the effect of inhibitors, and assessed the change in gene expression levels when exposed to radiation. GANT61 had a less toxic effect on the researched cell lines compared to cyclopamine. Cyclopamine is able to enhance the effect of radiation, but the effect depends on the cell culture.