cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
(4040) Patrick Sandeman
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Counteracting Signaling Activities in Lipid Rafts Associated with the Invasion of Lung Epithelial Cells by Pseudomonas aeruginosa
Creators: name: Wright, Jo Rae
name: Swan, Zachary D.
name: Sunday, Mary E.
name: Li, Guojie
name: Randell, Scott H.
name: Zaas, David W.
name: Degan, Simone
name: Abraham, Soman N.
name: Brown, Bethany J.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2009

description: Pseudomonas aeruginosa has the capacity to invade lung epithelial cells by co-opting the intrinsic endocytic properties of lipid rafts, which are rich in cholesterol, sphingolipids, and proteins, such as caveolin-1 and -2. We compared intratracheal Pseudomonas infection in wild type and caveolin-deficient mice to investigate the role of caveolin proteins in the pathogenesis of Pseudomonas pneumonia. Unlike wild type mice, which succumb to pneumonia, caveolin-deficient mice are resistant to Pseudomonas. We observed that Pseudomonas invasion of lung epithelial cells is dependent on caveolin-2 but not caveolin-1. Phosphorylation of caveolin-2 by Src family kinases is an essential event for Pseudomonas invasion. Our studies also reveal the existence of a distinct signaling mechanism in lung epithelial cells mediated by COOH-terminal Src kinase (Csk) that negatively regulates Pseudomonas invasion. Csk migrates to lipid raft domains, where it decreases phosphorylation of caveolin-2 by inactivating c-Src. Whereas Pseudomonas co-opts the endocytic properties of caveolin-2 for invasion, there also exists in these cells an intrinsic Csk-dependent cellular defense mechanism aimed at impairing this activity. The success of Pseudomonas in co-opting lipid raft-mediated endocytosis to invade lung epithelial cells may depend on the relative strengths of these counteracting signaling activities.
title: How far can ultrasound elastography help in diagnosis of prostatic lesions?
Creators: name: Emara, Doaa
Publisher: name: The Royal Australian and New Zealand College of Radiologists
Year: 2017
Subjects: subject: Genital / Reproductive system male
subject: Oncology
subject: Ultrasound
subject: Elastography
subject: Diagnostic procedure
subject: Cancer

description: Purpose Methods and materials Results Conclusion Personal information References
description: Purpose: Prostatic carcinoma is the most frequent malignancy in men and shows an increasing incidence. (1, 2) Increased prostate – specific antigen (PSA) could be seen in inflammatory conditions (acute and chronic prostatitis) beside malignancy. (2)...
Crystal Structures of Penicillin-binding Protein 2 from Penicillin-susceptible and -resistant Strains of Neisseria gonorrhoeae Reveal an Unexpectedly Subtle Mechanism for Antibiotic Resistance
Creators: name: Davies, Christopher
name: Nicholas, Robert A.
name: Deacon, Ashley M.
name: Powell, Ailsa J.
name: Tomberg, Joshua
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2009

description: Penicillin-binding protein 2 (PBP2) from N. gonorrhoeae is the major molecular target for β-lactam antibiotics used to treat gonococcal infections. PBP2 from penicillin-resistant strains of N. gonorrhoeae harbors an aspartate insertion after position 345 (Asp-345a) and 4-8 additional mutations, but how these alter the architecture of the protein is unknown. We have determined the crystal structure of PBP2 derived from the penicillin-susceptible strain FA19, which shows that the likely effect of Asp-345a is to alter a hydrogen-bonding network involving Asp-346 and the SXN triad at the active site. We have also solved the crystal structure of PBP2 derived from the penicillin-resistant strain FA6140 that contains four mutations near the C terminus of the protein. Although these mutations lower the second order rate of acylation for penicillin by 5-fold relative to wild type, comparison of the two structures shows only minor structural differences, with the positions of the conserved residues in the active site essentially the same in both. Kinetic analyses indicate that two mutations, P551S and F504L, are mainly responsible for the decrease in acylation rate. Melting curves show that the four mutations lower the thermal stability of the enzyme. Overall, these data suggest that the molecular mechanism underlying antibiotic resistance contributed by the four mutations is subtle and involves a small but measurable disordering of residues in the active site region that either restricts the binding of antibiotic or impedes conformational changes that are required for acylation by β-lactam antibiotics.
Extracting the depolarization coefficient D_NN from data measured with a full acceptance detector
Creators: name: Hauenstein, F.
name: Clement, H.
name: Dzhygadlo, R.
name: Eyrich, W.
name: Gillitzer, A.
name: Grzonka, D.
name: Jowzaee, S.
name: Ritman, J.
name: Roderburg, E.
name: Roeder, M.
name: Wintz, P.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Nuclear Experiment (nucl-ex)
subject: FOS: Physical sciences

description: The spin transfer from vertically polarized beam protons to Lambda or Sigma hyperons of the associated strangeness production pp -> pK Lambda (Sigma) is described with the depolarization coefficient D_NN. As the polarization of the hyperons is determined by their weak decays, detectors, which have a large acceptance for the decay particles, are needed. In this paper a formula is derived, which describes the depolarization coefficient D_NN by count rates of a 4 pi detector. It is shown, that formulas, which are given in publications for detectors with restricted acceptance, are specific cases of this formula for a 4 pi detector.
description: Accepted for publication by Nuclear Instruments and Methods in Physics Research Section A
title: Auswirkungen von Social Distancing auf die Teamarbeit
Creators: name: Dörler, Paula Virginia
Year: 2023

description: Anfang 2020 ist eine weltweite Pandemie ausgebrochen. Zunächst war China betroffen, andere Länder folgten zeitnah. Eine lokale Epidemie entwickelte sich rasant zu einer weltweiten Pandemie. Um die Pandemie einzudämmen, wurde als Maßnahme die soziale Distanzierung eingeführt. Jegliche Formen von sozialem Kontakt wurden für mehrere Monate eingeschränkt. Viele Beschäftigte arbeiten heutzutage in unterschiedlichen Teamformen zusammen. Teams sind weltweit zunehmend zu wichtigen gestaltenden Strukturelementen in Organisationen geworden. Projektteams sind eine spezifische Art von Teams, die kurzlebig sind, ein bestimmtes Projektthema bearbeiten, welches nicht routinemäßig ist, und die aus Teammitgliedern mit einem breiten Spektrum an Fertigkeiten, Expertise und Erfahrung bestehen (West et al., 2018). Projektteams sind speziell und aufgrund ihrer Besonderheiten, erfordert es ein besonderes Augenmerk, wie sich die soziale Distanzierung auf sie ausgewirkt hat. In dieser Masterarbeit wird der Problemstellung nachgegangen, welche Auswirkungen die soziale Distanzierung auf die Teamarbeit in Projektteams hat. Es wird eine fundierte, qualitative Studie angestrebt, die diesen Forschungsgegenstand untersucht. Die vorliegende Arbeit soll Auskunft darüber geben, ob und wie die soziale Distanzierung die Zusammenarbeit in Projektteams beeinflusst.
Density-enhanced Phosphatase 1 Regulates Phosphorylation of Tight Junction Proteins and Enhances Barrier Function of Epithelial Cells
Creators: name: Sallee, Jennifer L.
name: Burridge, Keith
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2009

description: Cell-cell adhesion is a dynamic process that can activate multiple signaling pathways. These signaling pathways can be regulated through reversible tyrosine phosphorylation events. The level of tyrosine phosphorylation of junctional proteins reflects the balance between protein-tyrosine kinase and protein-tyrosine phosphatase activity. The receptor-tyrosine phosphatase DEP-1 (CD148/PTP-η) has been implicated in cell growth and differentiation as well as in regulating phosphorylation of junctional proteins. However, the role of DEP-1 in regulating tight junction phosphorylation and the integrity of cell-cell junctions is still under investigation. In this study, we used a catalytically dead substrate-trapping mutant of DEP-1 to identify potential substrates at cell-cell junctions. We have shown that in epithelial cells the trapping mutant of DEP-1 interacts with the tight junction proteins occludin and ZO-1 in a tyrosine phosphorylation-dependent manner. In contrast, PTP-PEST, Shp2, and PTPμ did not interact with these proteins, suggesting that the interaction of DEP-1 with occludin and ZO-1 is specific. In addition, occludin and ZO-1 were dephosphorylated by DEP-1 but not these other phosphatases in vitro. Overexpression of DEP-1 increased barrier function as measured by transepithelial electrical resistance and also reduced paracellular flux of fluorescein isothiocyanate-dextran following a calcium switch. Reduced DEP-1 expression by small interfering RNA had a small but significant increase in junction permeability. These data suggest that DEP-1 can modify the phosphorylation state of tight junction proteins and play a role in regulating permeability.
(4034) Thomas Kean
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Engineering a Ubiquitin Ligase Reveals Conformational Flexibility Required for Ubiquitin Transfer
Creators: name: Chazin, Walter J.
name: Waldron, Lauren
name: Choudhary, Neelima
name: Patterson, Cam
name: Klevit, Rachel E.
name: Qian, Shu Bing
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2009

description: Protein ubiquitination regulates numerous cellular functions in eukaryotes. The prevailing view about the role of RING or U-box ubiquitin ligases (E3) is to provide precise positioning between the attached substrate and the ubiquitin-conjugating enzyme (E2). However, the mechanism of ubiquitin transfer remains obscure. Using the carboxyl terminus of Hsc70-interacting protein as a model E3, we show herein that although U-box binding is required, it is not sufficient to trigger the transfer of ubiquitin onto target substrates. Furthermore, additional regions of the E3 protein that have no direct contact with E2 play critical roles in mediating ubiquitin transfer from E2 to attached substrates. By combining computational structure modeling and protein engineering approaches, we uncovered a conformational flexibility of E3 that is required for substrate ubiquitination. Using an engineered version of the carboxyl terminus of Hsc70-interacting protein ubiquitin ligase as a research tool, we demonstrate a striking flexibility of ubiquitin conjugation that does not affect substrate specificity. Our results not only reveal conformational changes of E3 during ubiquitin transfer but also provide a promising approach to custom-made E3 for targeted proteolysis.
title: Treatment outcome of post-prostatectomy intensity modulated radiotherapy: Tomotherapy vs. Linear Accelerator
Creators: name: Qin, Jason
Publisher: name: The Royal Australian and New Zealand College of Radiologists
Year: 2017
Subjects: subject: Intensity Modulated Radiotherapy (IMRT)
subject: VMAT and Tomotherapy
subject: Radiation therapy / Oncology
subject: CT
subject: Pelvis
subject: Oncology
subject: Outcomes
subject: Toxicity

description: Purpose Methods and materials Results Conclusion Personal information References
description: Purpose: To compare biochemical progression free outcomes and toxicity rates of prostate cancer patients who have undergone post-prostatectomy intensity modulated radiotherapy via Tomotherapy (Tomo) or Intensity Modulated Radiotherapy on Linear Accelerator...
Abnormal Synchronizing Path of Delay-coupled Chaotic Oscillators on the Edge of Stability
Creators: name: Zhuo, Zhao
name: Cai, Shi-Min
name: Fu, Zhong-Qian
Publisher: name: arXiv
Year: 2015
Subjects: subject: Physics and Society (physics.soc-ph)
subject: FOS: Physical sciences

description: In this paper, the transition of synchronizing path of delay-coupled chaotic oscillators in a scale-free network is highlighted. Mainly, through the critical transmission delay makes chaotic oscillators be coupled on the edge of stability, we find that the transition of synchronizing path is \emph{abnormal}, which is characterized by the following evidences: (a) synchronization process starts with low-degree rather than high-degree ones; (b) the high-degree nodes don't undertake the role of hub; (c) the synchronized subnetworks show a poor small-world property as a result of hubs absence; (d) the clustering synchronization behavior emerges even community structure is absent in the scale-free network. This abnormal synchronizing path suggests that the diverse synchronization behaviors occur in the same topology, which implies that the relationship between dynamics and structure of network is much more complicated than the common sense that the structure is the foundation of dynamics. Moreover, it also reveals the potential connection from the transition of synchronization behavior to disorder in real complex networks, e.g. Alzheimer disease.
description: 12 pages, 4 figures
Human-in-Loop: A Review of Smart Manufacturing Deployments
Creators: name: Bhattacharya, Mangolika
name: Penica, Mihai
name: O'CONNELL, EOIN
name: SOUTHERN, MARK
name: HAYES, MARTIN
Publisher: name: University of Limerick
Year: 2023
Subjects: Engineering

description: The recent increase in computational capability has led to an unprecedented increase in the range of new applications where machine learning can be used in real time. Notwithstanding the range of use cases where automation is now feasible, humans are likely to retain a critical role in the operation and certification of manufacturing systems for the foreseeable future. This paper presents a use case review of how human operators affect the performance of cyber–physical systems within a ’smart’ or ’cognitive’ setting. Such applications are classified using Industry 4.0 (I4.0) or 5.0 (I5.0) terminology. The authors argue that, as there is often no general agreement as to when a specific use case moves from being an I4.0 to an I5.0 example, the use of a hybrid Industry X.0 notation at the intersection between I4.0 and I5.0 is warranted. Through a structured review of the literature, the focus is on how secure human-mediated autonomous production can be performed most effectively to augment and optimise machine operation.
(4035) Ann Jane Kean
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Issues in the Multiple Try Metropolis mixing
Creators: name: Martino, L.
name: Louzada, F.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Computation (stat.CO)
subject: FOS: Computer and information sciences

description: The multiple Try Metropolis (MTM) algorithm is an advanced MCMC technique based on drawing and testing several candidates at each iteration of the algorithm. One of them is selected according to certain weights and then it is tested according to a suitable acceptance probability. Clearly, since the computational cost increases as the employed number of tries grows, one expects that the performance of an MTM scheme improves as the number of tries increases, as well. However, there are scenarios where the increase of number of tries does not produce a corresponding enhancement of the performance. In this work, we describe these scenarios and then we introduce possible solutions for solving these issues.
About the record of the black-bellied angler, Lophius budegassa Spinola, 1807, from Sinop coast in the Black Sea, Turkey
Creators: name: SUMER, Cetin
name: BILGIN, Sabri
name: BEKTAS, Serdar
name: SATILMIS, Hasan Huseyin
Publisher: name: Station Biologique de Roscoff (SBR)
Year: 2016

description: One specimen of the black-bellied angler, Lophius budegassa Spinola, 1807, was caught off Sinop (Black Sea, Turkey) on 8 February 2015 by turbot bottom set net. The specimen weighed 1926.6 g and measured 42.2 cm standard length (SL) and 51.8 cm total length (TL). The specimen sexed as a maturing male with a testicular development stage II. This work has undoubtedly enhanced our knowledge on the recent introduction of the black-bellied angler in the Black Sea.
G Protein Mono-ubiquitination by the Rsp5 Ubiquitin Ligase
Creators: name: Lee, Michael J.
name: Kuhlman, Brian
name: Dokholyan, Nikolay V.
name: Ding, Feng
name: Torres, Matthew P.
name: Purbeck, Carrie
name: Dohlman, Henrik G.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2009

description: Emerging evidence suggests that ubiquitination serves as a protein trafficking signal in addition to its well characterized role in promoting protein degradation. The yeast G protein α subunit Gpa1 represents a rare example of a protein that undergoes both mono- and poly-ubiquitination. Whereas mono-ubiquitinated Gpa1 is targeted to the vacuole, poly-ubiquitinated Gpa1 is directed instead to the proteasome. Here we investigate the structural requirements for mono- and poly-ubiquitination of Gpa1. We find that variants of Gpa1 engineered to be unstable are more likely to be poly-ubiquitinated and less likely to be mono-ubiquitinated. In addition, mutants that cannot be myristoylated are no longer mono-ubiquitinated but are still polyubiquitinated. Finally, we show that the ubiquitin ligase Rsp5 is necessary for Gpa1 mono-ubiquitination in vivo and that the purified enzyme is sufficient to catalyze Gpa1 mono-ubiquitination in vitro. Taken together, these data indicate that mono- and poly-ubiquitination have distinct enzyme and substrate recognition requirements; whereas poly-ubiquitination targets misfolded protein for degradation, a distinct ubiquitination apparatus targets the fully mature, fully myristoylated G protein for mono-ubiquitination and delivery to the vacuole.
title: Fiducial-free Liver SBRT using exhale breath-hold motion management
Creators: name: Hilleary, Liam
Publisher: name: The Royal Australian and New Zealand College of Radiologists
Year: 2017
Subjects: subject: Liver
subject: Abdomen
subject: Oncology
subject: Cone beam CT
subject: Fluoroscopy
subject: Image manipulation / Reconstruction
subject: Ablation procedures
subject: Radiation therapy / Oncology
subject: Stereotactic radiotherapy
subject: Image guided radiotherapy
subject: Radiotherapy techniques
subject: Artifacts

description: Purpose Methods and materials Results Conclusion Personal information References
description: Purpose: Fiducial markers can help with the accurate delivery of high dose radiation treatments in organs such as the liver. But can taking measures to restrict organ motion and reduce artefact, improve imaging quality enough to avoid the use of invasive...
Soft bottom Sipunculans in the Sea of Marmara
Creators: name: AÇIK, Sermin
Publisher: name: Station Biologique de Roscoff (SBR)
Year: 2016

description: This study deals with the sipunculan species collected on soft substrata in the Sea of Marmara. The faunistic analysis of 10 benthic stations taken from 5 to 68 m depths yielded 9 sipunculan species and 237 individuals belonging to 6 genera. Four species, Golfingia (Golfingia) elongata, Golfingia (Golfingia) vulgaris vulgaris, Nephasoma (Nephasoma) constrictum and Aspidosiphon (Aspidosiphon) mexicanus, are new to the fauna of the Sea of Marmara. Onchnesoma steenstrupii steenstrupii is the most abundant (78.9%) and frequent species (60%) in the area. The soft bottom community included one alien species: Aspidosiphon (Akrikos) mexicanus. Brief descriptions of the species and their morphological, biometrical and distributional characteristics are given.
title: WCAG-Barrierefreiheitstandards in Content Management Systeme
Creators: name: Feirer, David
Publisher: name: FH CAMPUS 02 (CAMPUS 02 Fachhochschule der Wirtschaft)
Year: 2025
Subjects: subject: 658 Allgemeines Management

description: Im Rahmen der vorliegenden Masterarbeit wurden die Herausforderungen bei der Implementierung der Web Content Accessibility Guidelines (WCAG) bei Verwendung von Content Management Systemen (CMS) analysiert, sowie Lösungen für jedes identifizierte Problem vorgestellt. Mithilfe der durchgeführten systematischen Literaturrecherche (SLR) nach vom Brocke et al. (2009) wurden eine Vielzahl an relevanten Quellen zum Thema umfassend analysiert. Basierend auf den Ergebnissen der SLR wurden drei Hauptprobleme identifiziert. Diese beinhalten die allgemeine technische Herausforderung das CMS an einen Standard anzupassen, die Benutzerfreundlichkeit für Content-Ersteller*innen und die zeitliche Herausforderung der Implementierung. Für jedes dieser Probleme werden im Rahmen dieser Arbeit spezifische Lösungen vorgestellt. Darüber hinaus bietet die durchgeführte SLR einen Überblick über die Vor- und Nachteile, bei einer Umsetzung der WCAG-Standards sowie eine Einschätzung der Einstellung gegenüber der Umsetzung von WCAG-Richtlinien mithilfe von CMS. Insgesamt zeigt die Arbeit, nicht nur, dass bei der Implementierung von WCAG-Standards in CMS technische sowie organisatorische Herausforderungen auftreten, sondern präsentiert auch umfassende Lösungskonzepte und gute Orientierungsmöglichkeiten, um eine zugänglichere digitale Welt zu erreichen.
description: This Master's thesis dives into the challenges associated with implementing the Web Content Accessibility Guidelines (WCAG) using Content Management Systems (CMS). It presents a comprehensive analysis based on a systematic literature review (SLR) as proposed by vom Brocke et al. (2009). Through the findings of the SLR, three primary challenges were identified: the technical difficulties in adapting CMS to meet standards, improving usability for content creators and the time constraints involved in implementation. For each identified challenge, this thesis introduces specific solutions. In addition, the SLR conducted offers insights into the advantages and disadvantages of applying WCAG standards, along with an evaluation of attitudes towards the adoption of these guidelines through CMS platforms. In essence, this thesis not only highlights the technical and organizational challenges in integrating WCAG standards into CMS but also provides extensive solutions to overcome these challenges. By doing so, it contributes towards achieving a more accessible digital world.
Human Heart Mitochondrial DNA Is Organized in Complex Catenated Networks Containing Abundant Four-way Junctions and Replication Forks
Creators: name: Karhunen, Pekka J.
name: Jacobs, Howard T.
name: Suomalainen, Anu
name: Tyynismaa, Henna
name: Holt, Ian J.
name: Pohjoismäki, Jaakko L.O.
name: Ide, Tomomi
name: Willcox, Smaranda
name: Griffith, Jack D.
name: Kang, Dongchon
name: Goffart, Steffi
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2009

description: Analysis of human heart mitochondrial DNA (mtDNA) by electron microscopy and agarose gel electrophoresis revealed a complete absence of the θ-type replication intermediates seen abundantly in mtDNA from all other tissues. Instead only Y- and X-junctional forms were detected after restriction digestion. Uncut heart mtDNA was organized in tangled complexes of up to 20 or more genome equivalents, which could be resolved to genomic monomers, dimers, and linear fragments by treatment with the decatenating enzyme topoisomerase IV plus the cruciform-cutting T7 endonuclease I. Human and mouse brain also contained a population of such mtDNA forms, which were absent, however, from mouse, rabbit, or pig heart. Overexpression in transgenic mice of two proteins involved in mtDNA replication, namely human mitochondrial transcription factor A or the mouse Twinkle DNA helicase, generated abundant four-way junctions in mtDNA of heart, brain, and skeletal muscle. The organization of mtDNA of human heart as well as of mouse and human brain in complex junctional networks replicating via a presumed non-θ mechanism is unprecedented in mammals.
Lipid Binding to the Tail Domain of Vinculin: SPECIFICITY AND THE ROLE OF THE N AND C TERMINI
Creators: name: Craig, Susan W.
name: Playford, Martin P.
name: Palmer, Sean M.
name: Campbell, Sharon L.
name: Schaller, Michael D.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2009

description: Vinculin is a highly conserved and abundant cytoskeletal protein involved in linking the actin cytoskeleton to the cell membrane at sites of cellular adhesion. At these sites of adhesion, vinculin plays a role in physiological processes such as cell motility, migration, development, and wound healing. Loss of normal vinculin function has been associated with cancer phenotypes, cardiovascular disease, and lethal errors in embryogenesis. The tail domain of vinculin (Vt) binds to acidic phospholipids and has been proposed to play a role in vinculin activation and focal adhesion turnover. To better characterize Vt-lipid specificity, we conducted a series of lipid co-sedimentation experiments and find that Vt shows specific association with phosphatidylinositol 4,5-bisphosphate (PIP2), compared with phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylserine (PS), or phosphatidylinositol (PI) in the context of mixed lipid vesicles. The C terminus of Vt has been proposed to be important for PIP2 association, as various mutations and deletions within the C-terminal reduce PIP2 association. Lipid co-sedimentation and NMR analyses indicate that removal of the hydrophobic hairpin does not alter Vt structure or PIP2 association. However, more extensive deletions within the C-terminal introduce Vt structural perturbations and reduce PIP2 binding. Intriguingly, a significant increase in PIP2 binding was observed for multiple Vt variants that perturb interactions between the N-terminal strap and helix bundle, suggesting that a rearrangement of this N-terminal strap may be required for PIP2 binding.
Liver-specific Deficiency of Serine Palmitoyltransferase Subunit 2 Decreases Plasma Sphingomyelin and Increases Apolipoprotein E Levels
Creators: name: Fan, Yifan
name: Kuo, Ming Shang
name: Bui, Hai H.
name: Chakraborty, Mahua
name: Li, Yan
name: Jiang, Xian Cheng
name: Cao, Guoqing
name: Li, Zhiqiang
name: Xiao, Xiao
name: Peake, David A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2009

description: Sphingomyelin (SM) is one of the major lipid components of plasma lipoproteins. Serine palmitoyltransferase (SPT) is the key enzyme in SM biosynthesis. Mice totally lacking in SPT are embryonic lethal. The liver is the major site for plasma lipoprotein biosynthesis, secretion, and degradation, and in this study we utilized a liver-specific knock-out approach for evaluating liver SPT activity and also its role in plasma SM and lipoprotein metabolism. We found that a deficiency of liver-specific Sptlc2 (a subunit of SPT) decreased liver SPT protein mass and activity by 95 and 92%, respectively, but had no effect on other tissues. Liver Sptlc2 deficiency decreased plasma SM levels (in both high density lipoprotein and non-high density lipoprotein fractions) by 36 and 35% (p < 0.01), respectively, and increased phosphatidylcholine levels by 19% (p < 0.05), thus increasing the phosphatidylcholine/SM ratio by 77% (p < 0.001), compared with controls. This deficiency also decreased SM levels in the liver by 38% (p < 0.01) and in the hepatocyte plasma membranes (based on a lysenin-mediated cell lysis assay). Liver-specific Sptlc2 deficiency significantly increased hepatocyte apoE secretion and thus increased plasma apoE levels 3.5-fold (p < 0.0001). Furthermore, plasma from Sptlc2 knock-out mice had a significantly stronger potential for promoting cholesterol efflux from macrophages than from wild-type mice (p < 0.01) because of a greater amount of apoE in the circulation. As a result of these findings, we believe that the ability to control liver SPT activity could result in regulation of lipoprotein metabolism and might have an impact on the development of atherosclerosis.
Additional file 1 of The views of people living with chronic stroke and aphasia on their potential involvement as research partners: a thematic analysis
Creators: name: Charalambous, Marina
name: Kountouri, Alexia
name: Phylactou, Phivos
name: Triantafyllidou, Ioanna
name: Annoni, Jean-Marie
name: Kambanaros, Maria
Publisher: name: figshare
Year: 2022
Subjects: subject: Neuroscience
subject: Biotechnology
subject: Science Policy
subject: Immunology
subject: FOS: Clinical medicine
subject: 111714 Mental Health
subject: FOS: Health sciences

description: Additional file 1. Sample consent forms.
title: Gabb’s Law: Thou shalt NOT have gas over thy liver!!
Creators: name: Constantine, Sarah
Publisher: name: The Royal Australian and New Zealand College of Radiologists
Year: 2017
Subjects: subject: Abdomen
subject: Gastrointestinal tract
subject: Conventional radiography
subject: CT
subject: Diagnostic procedure
subject: Education
subject: Ischaemia / Infarction
subject: Inflammation
subject: Infection

description: Learning objectives Background Imaging findings OR Procedure details Conclusion Personal information References
description: Learning objectives: To recognise the presence and significance of gas projected in the right upper quadrant on plain abdominal...
(4032) Catherine kean
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Cavity-mediated coupling of mechanical oscillators limited by quantum backaction
Creators: name: Spethmann, Nicolas
name: Kohler, Jonathan
name: Schreppler, Sydney
name: Buchmann, Lukas
name: Stamper-Kurn, Dan M.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Quantum Physics (quant-ph)
subject: Quantum Gases (cond-mat.quant-gas)
subject: Atomic Physics (physics.atom-ph)
subject: Optics (physics.optics)
subject: FOS: Physical sciences

description: A complex quantum system can be constructed by coupling simple quantum elements to one another. For example, trapped-ion or superconducting quantum bits may be coupled by Coulomb interactions, mediated by the exchange of virtual photons. Alternatively quantum objects can be coupled by the exchange of real photons, particularly when driven within resonators that amplify interactions with a single electro-magnetic mode. However, in such an open system, the capacity of a coupling channel to convey quantum information or generate entanglement may be compromised. Here, we realize phase-coherent interactions between two spatially separated, near-ground-state mechanical oscillators within a driven optical cavity. We observe also the noise imparted by the optical coupling, which results in correlated mechanical fluctuations of the two oscillators. Achieving the quantum backaction dominated regime opens the door to numerous applications of cavity optomechanics with a complex mechanical system. Our results thereby illustrate the potential, and also the challenge, of coupling quantum objects with light.
description: 25 pages, 6 figures
First record of the spotted unicornfish Naso brevirostris (Cuvier, 1829) (Teleostei: Acanthuridae) in the Sea of Oman
Creators: name: AL-MAMRY, Juma M.
name: JAWAD, Laith A.
name: AL-MARZOUQI, Abdulaziz
name: AL-BUSAIDI, Haithem K.
Publisher: name: Station Biologique de Roscoff (SBR)
Year: 2016

description: The first record of a single specimen of the spotted unicornfish, Naso brevirostris is reported from the Omani coast of Sea of Oman. The specimen was caught from the coast of Qurait within the Sea of Oman during an ichthyologic survey on the 23rd August 2015. The capture of an adult specimen, 330 mm in total length, marks the first record of a member of the genus Naso in the Sea of Oman. Several factors may explain the presence of this species in Omani waters.
Galerkin Method for the numerical solution of the RLW equation by using exponential B-splines
Creators: name: Görgülü, M. Z.
name: Dağ, İ.
name: Irk, D.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Numerical Analysis (math.NA)
subject: FOS: Mathematics

description: In this paper, the exponential B-spline functions are used for the numerical solution of the RLW equation. Three numerical examples related to propagation of single solitary wave, interaction of two solitary waves and wave generation are employed to illustrate the accuracy and the efficiency of the method. Obtained results are compared with some early studies.
Liver-specific Loss of Long Chain Acyl-CoA Synthetase-1 Decreases Triacylglycerol Synthesis and β-Oxidation and Alters Phospholipid Fatty Acid Composition
Creators: name: Watkins, Steven M.
name: Altshuller, George
name: Thresher, Randy J.
name: Koves, Timothy R.
name: Muoio, Deborah M.
name: Cline, Gary W.
name: Shulman, Gerald I.
name: Paich, Heather A.
name: Gong, Nan
name: Ellis, Jessica M.
name: Coleman, Rosalind A.
name: Wang, Shuli
name: Li, Lei O.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2009

description: In mammals, a family of five acyl-CoA synthetases (ACSLs), each the product of a separate gene, activates long chain fatty acids to form acyl-CoAs. Because the ACSL isoforms have overlapping preferences for fatty acid chain length and saturation and are expressed in many of the same tissues, the individual function of each isoform has remained uncertain. Thus, we constructed a mouse model with a liver-specific knock-out of ACSL1, a major ACSL isoform in liver. Eliminating ACSL1 in liver resulted in a 50% decrease in total hepatic ACSL activity and a 25–35% decrease in long chain acyl-CoA content. Although the content of triacylglycerol was unchanged in Acsl1L−/− liver after mice were fed either low or high fat diets, in isolated primary hepatocytes the absence of ACSL1 diminished the incorporation of [14C]oleate into triacylglycerol. Further, small but consistent increases were observed in the percentage of 16:0 in phosphatidylcholine and phosphatidylethanolamine and of 18:1 in phosphatidylethanolamine and lysophosphatidylcholine, whereas concomitant decreases were seen in 18:0 in phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and lysophosphatidylcholine. In addition, decreases in long chain acylcarnitine content and diminished production of acid-soluble metabolites from [14C]oleate suggested that hepatic ACSL1 is important for mitochondrial β-oxidation of long chain fatty acids. Because the Acsl1L−/− mice were not protected from developing either high fat diet-induced hepatic steatosis or insulin resistance, our study suggests that lowering the content of hepatic acyl-CoA without a concomitant decrease in triacylglycerol and other lipid intermediates is insufficient to protect against hepatic insulin resistance.
Modification of light transmission channels by inhomogeneous absorption in random media
Creators: name: Liew, Seng Fatt
name: Cao, Hui
Publisher: name: arXiv
Year: 2015
Subjects: subject: Optics (physics.optics)
subject: FOS: Physical sciences

description: Optical absorption is omnipresent and very often distributed non-uniformly in space. We present a numerical study on the effects of inhomogeneous absorption on transmission eigenchannels of light in highly scattering media. In the weak absorption regime, the spatial profile of a transmission channel remains very similar to that without absorption, and the effect of inhomogeneous absorption can be stronger or weaker than homogeneous absorption depending on the spatial overlap of the localized absorbing region with the field intensity maximum of the channel. In the strong absorption regime, the high transmission channels redirect the energy flows to circumvent the absorbing regions to minimize loss. The attenuation of high transmission channels by inhomogeneous absorption is lower than that by homogeneous absorption, regardless of the location of the absorbing region. The statistical distribution of transmission eigenvalues in the former becomes broader than that in the latter, due to a longer tail at high transmission. Since the maximum transmission channel is the most efficient in bypassing the absorbing region, the ratio of its transmittance to the average transmittance increases with absorption, eventually exceeds the ratio without absorption.
description: 11 pages, 4 figures
Visualization and analysis of the Kohn-Sham kinetic energy density and its orbital-free description in molecules
Creators: name: Cancio, Antonio C.
name: Stewart, Dane
name: Kuna, Aeryk I.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Chemical Physics (physics.chem-ph)
subject: Materials Science (cond-mat.mtrl-sci)
subject: FOS: Physical sciences

description: We visualize the Kohn-Sham kinetic energy density (KED), and the ingredients -- the electron density, its gradient and Laplacian -- used to construct orbital-free models of it, for the AE6 test set of molecules. These are compared to related quantities used in metaGGA's, to characterize two important limits -- the gradient expansion and the localized-electron limit typified by the covalent bond. We find the second-order gradient expansion of the KED to be a surprisingly successful predictor of the exact KED, particularly at low densities where this approximation fails for exchange. This contradicts the conjointness conjecture that the optimal enhancement factors for orbital-free kinetic and exchange energy functionals are identical. In addition we find significant problems with a recent metaGGA-level orbital-free KED, especially for regions of strong electron localization. We define an orbital-free description of electron localization and a revised metaGGA that improves upon atomization energies significantly.
description: 18 pages, 10 figures
title: Die Einteilung der Erde in Zonobiome
Creators: name: Sporn, David Johannes
Year: 2023

description: In der vorliegenden Arbeit wird das Zonobiomkonzept von Heinrich Walter und Siegmar Breckle beschrieben, das eine Gliederung der Erde in neun Gebiete mit jeweils sehr ähnlichen klimatischen Bedingungen darstellt, die eine charakteristische Ausprägung der Vegetation aufweisen. Diese resultiert meist aus der Anpassung an die unterschiedlichen Standortbedingungen. Darauf aufbauend wurde ein Gestaltungskonzept entworfen, das die einzelnen Zonobiome im Bestand des Botanischen Gartens der Universität Wien präsentiert. Den Mittelpunkt bildet eine mit charakteristischen Arten bepflanzte, begehbare Weltkarte, bei der die Theorie des Zonobiom-Konzepts erklärt wird. Neun weitere mit Schautafeln versehene Standorte geben den BesucherInnen einen detaillierteren Einblick in die Ausprägung der jeweiligen Zonobiom-Vegetation. Durch eine didaktische Aufbereitung des Gestaltungskonzepts in Form einer Führung und eines Workshops für SchülerInnen wird das Zonobiomkonzept einem breiten Publikum vermittelt.
description: Walter’s and Breckle’s geobotanical zonobiome concept represents the ecological structure of the earth. There are nine zonobiomes which comprise climatically similar areas, with each having a characteristic vegetation as adaption to different temperatures, frost and precipitation. In this master thesis a design concept was developed in order to present the zonobiomes in the botanical garden of the University of Vienna. The core of the project is a world map planted with characteristic species, where the theory of the zonobiome concept is explained. This world map is accessible to visitors. At nine further locations the visitors obtain detailed insights into the characteristics of each zonobiome vegetation. The zonobiome concept is brought to the broader public through didactic tools in form of a guided tour and a workshop for students.
(4033) Julia Kean
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
ESPÈCES NOUVELLES DE LEPTOLAIMIDAE (NEMATODA) ET DESCRIPTION DU GENRE LEPTOLAIMOIDES N. GEN.
Creators: name: VITIELLO, Pierre
Publisher: name: Station Biologique de Roscoff (SBR)
Year: 1971

description: Five new forms of Leptolaimidae are described. Antomicron profundum n. sp. is characterized by presence of cephalic setae and important number of papillae and preanal tubular organs. Leptolaimus alatus n. sp. is distinguished by the number of preanal tubular supplements. Leptolaimus minutus n. sp. has cephalic setae but no papillae or preanal tubular organs. Leptolaimus timmi n. sp. is différent of other species by the size, the measures of amphids and cephalic setae and the presence of ten or eleven tubular supplements. The genus Leptolaimoides n.gen. is established for Leptolaimidae forms that differ from Leptolaimus byvery elongated amphids; two species: Leptolaimoides thermastris (Lorenzen, 1966) nov. nom. without preanal supplements and Leptolaimoides tubulosus n. sp. which has tubular supplements.
Lysine 88 Acetylation Negatively Regulates Ornithine Carbamoyltransferase Activity in Response to Nutrient Signals
Creators: name: Xiong, Yue
name: Yu, Wei
name: Yao, Jun
name: Huang, Wei
name: Zhao, Shimin
name: Lei, Qunying
name: Guan, Kun Liang
name: Lin, Yan
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2009

description: Ornithine carbamoyltransferase (OTC) is a key enzyme in the urea cycle to detoxify ammonium produced from amino acid catabolism. OTC deficiency is an X-linked genetic disorder ranging from fatal in newborns to hyperammonemia and anorexia in adults. Through affinity purification of acetylated peptides and mass spectrometry, we identified that OTC is acetylated on lysine residues, including Lys88, which is also mutated in OTC-deficient patients. OTC acetylation was confirmed to occur under physiological conditions. Biochemical characterizations revealed that OTC Lys88 acetylation decreases the affinity for carbamoyl phosphate, one of the two OTC substrates, and the maximum velocity, whereas the Km for ornithine, the other OTC substrate, is not affected. Furthermore, Lys88 acetylation is regulated by both extracellular glucose and amino acid availability, indicating that OTC activity may be regulated by cellular metabolic status. Our results provide an example of the novel mechanism of regulating metabolic enzyme activity through protein acetylation.
An investigation into the mathematical needs of school leavers entering craft apprenticeships and, based upon the information gained, the preparation of relevant material for use within schools
Creators: name: Bond, Rodney M.
Publisher: name: Loughborough University
Year: 2020
Subjects: subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics

description: An investigation into the mathematical needs of school leavers entering craft apprenticeships and, based upon the information gained, the preparation of relevant material for use within schools
Influence network in Chinese stock market
Creators: name: Gao, Ya-Chun
name: Zeng, Yong
name: Cai, Shi-Min
Publisher: name: arXiv
Year: 2015
Subjects: subject: Physics and Society (physics.soc-ph)
subject: Data Analysis, Statistics and Probability (physics.data-an)
subject: Statistical Finance (q-fin.ST)
subject: FOS: Physical sciences
subject: FOS: Economics and business

description: In a stock market, the price fluctuations are interactive, that is, one listed company can influence others. In this paper, we seek to study the influence relationships among listed companies by constructing a directed network on the basis of Chinese stock market. This influence network shows distinct topological properties, particularly, a few large companies that can lead the tendency of stock market are recognized. Furthermore, by analyzing the subnetworks of listed companies distributed in several significant economic sectors, it is found that the influence relationships are totally different from one economic sector to another, of which three types of connectivity as well as hub-like listed companies are identified. In addition, the rankings of listed companies obtained from the centrality metrics of influence network are compared with that according to the assets, which gives inspiration to uncover and understand the importance of listed companies in the stock market. These empirical results are meaningful in providing these topological properties of Chinese stock market and economic sectors as well as revealing the interactively influence relationships among listed companies.
description: 14 pages, 7 Figures