Pestalotiopsis linguae Li Hua, Manawas., &amp; YX Zhang. 2023, sp. nov.
Creators: name: Li, Hua
name: Manawasinghe, Ishara S.
name: Zhang, Yunxia
name: Senanayake, Indunil C.
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Fungi
Ascomycota
Sordariomycetes
Amphisphaeriales
Pestalotiopsidaceae
Pestalotiopsis
Pestalotiopsis linguae

description: <i>Pestalotiopsis linguae</i> Li Hua, Manawas., &amp; YX Zhang. <i>sp. nov</i>. (FIGURE 3). Index Fungourum number: IF 559839; Facesoffungi number: FOF 12712. Etymology: Epithet refers to the host species from which the fungus was isolated Holotype: ZHKU 22-0089 <i>Endophytic</i> on the <i>Pyrrosia lingua</i> (<i>Polypodiaceae</i>) leaves. <i>Sexual morph</i>: not observed. <i>Asexual morph</i>: <i>Conidiomata acervular</i>, 800–1,100 μm diameter, globose, solitary or aggregated in clusters, exuding black conidial masses. <i>Conidiophores</i> reduced to conidiogenous cells. <i>Conidiogenous cells</i> discrete or integrated, cylindrical to subcylindrical, hyaline, smooth, annelidic. <i>Conidia</i> (16.5–)18–21(–23) × (4–)5–7(–8) μm (x̅±SD=19.2±1.4 × 5.9±0.8 μm, n=30), fusoid, ellipsoid, straight or slightly curved, 4-septate; basal cell conical, (2–)3–5 μm, hyaline, smooth, thin-walled; with a single appendage (3–)4–7(–8) μm (x̅±SD=5±1.3 μm, n=30) long, filiform, unbranched, centric; three median cellsdoliiform, (11–)11.5–13(–14) μm (x̅±SD=12.2±0.7 μm, n=30) long, smooth, concolorous, brown, septa darker than the restof the cell (second cell from base (3–)4–5 μm long; third cell (2–)3–4(–5) μm long; fourth cell (3–)4–5 μm long); apical cell conical, (2.5–)3–4(–4.5) μm (x̅±SD=3.7±0.5 μm, n=30) long, hyaline, smooth, thin-walled; with 2–3 apicaltubular appendages (10–)12–17(–21.5) μm (x̅±SD=14.9±2.5 μm, n=30) long, unbranched, filiform. <i>Culture characteristics</i>: Colonies on PDA reaching 50 mm diameter after five days at 25 ℃. Colonies filamentous to circular, medium dense, white aerial mycelium on surface flat or raised. <i>Material examined</i>: CHINA, Guangdong Province, Guangzhou City, from the leaf of <i>Pyrrosia lingua</i> (Thunb.) Farwell (23°11’13”N 113°17’51”E), 16 August. 2021, Li Hua (holotype ZHKU 22-0089 as dry culture); ex-type living culture ZHKUCC 22-0159, <i>ibid</i> ZHKUCC 22-0160. <i>Notes</i>: Two isolates in this study developed a clustered with 78% ML and 1.00 BYPP values. In the phylogenetic tree, our isolates show a particular neighbouring to seven known <i>Pestalotipsis</i> species, but they differ in morphology. The length of the base appendage of <i>P. linguae</i> is longer than that of <i>P. formosana</i> (4–7 μm vs. 3–5 μm), <i>P. kandelicola</i> (4–7 μm vs. 2.5–3 μm), <i>P. parva</i> (4–7 μm vs. 2–4 μm), and <i>P. etonensis</i> (4–7 μm vs. 2–4.5 μm). The length of conidia of <i>P. linguae</i> is shorter than that of <i>P. kandelicola</i> (18–21 μm vs. 20–23.5 μm), <i>P. nanningensis</i> (18–21 μm vs. 24–26.5 μm), <i>P. thailandica</i> (18–21 μm vs. 21–25 μm), <i>P. rhizophorae</i> (18–21 μm vs. 20.5–27 μm), but its conidia is longer than <i>P. etonensis</i> (18–21 μm vs. 15–21 μm). The apical appendage of <i>P. linguae</i> is longer than that of <i>P. nanningensis</i> (12–17 μm). vs. 18–22.5 μm) shorter and longer than <i>P. parva</i> (12–17 μm vs. 6.5–12 μm). In addition, <i>P. lingua</i> can also differ by <i>P. formosana</i> based on the base pair differences (2/ 523 in ITS, 3/ 452 in <i>tef</i> 1-α and 3/ 692 in <i>tub2</i>, no gaps). A strict morphological comparison of our isolates and their phylogenetically related species is presented in <b>TABLE 2</b>. Furthermore, the PHI analysis showed no evidence of genetic recombination between <i>P. linguae</i> and <i>P. formosana</i>, <i>P. nanningensis</i>, <i>P. parva</i>, or <i>P. kandelicola</i>. Therefore, the collection in this study is designated as a new species.
description: Published as part of <i>Li, Hua, Manawasinghe, Ishara S., Zhang, Yunxia &amp; Senanayake, Indunil C., 2023, Taxonomic and phylogenic appraisal of Pestalotiopsis linguae sp. nov., and a new record of P. nanjingensis from Pyrrosia lingua (Polypodiaceae) in Southern China, pp. 229-250 in Phytotaxa 587 (3)</i> on pages 241-242, DOI: 10.11646/phytotaxa.587.3.3, http://zenodo.org/record/7744311