Dothiorella albiziae Rathnayaka & K. D. Hyde 2022, sp. nov.
Creators: name: Rathnayaka, Achala R.
name: Chethana, K. W. Thilini
name: Phillips, Alan J. L.
name: Jones, E. B. Gareth
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Fungi
Ascomycota
Sordariomycetes
Diaporthales
Diaporthaceae
Dothiorella
Dothiorella albiziae

description: Dothiorella albiziae Rathnayaka & K.D. Hyde, sp. nov.
Index Fungorum number: IF559720; Facesoffungi number: FoF 11669; Fig. 6.
Etymology:—In reference to Albizia lebbeck, the host from which the fungus was collected.
Holotype:— MFLU 22-0093
Saprobic on dead Albizia lebbeck dry pod. Sexual morph: Undetermined. Asexual morph: Coelomycetous. Conidiomata 310–340 μm high × 250–265 μm diam. (x̄ = 320 × 262 μm, n = 10), pycnidial, solitary or gregarious, globose, formed in uniloculate stromata, immersed, becoming erumpent at maturity. Conidiomata wall 24–68 μm diam., of two layers, outer layer composed of thick-walled, dark brown to brown cells of textura angularis, inner layer composed of thin-walled, pale brown to hyaline cells of textura angularis. Conidiophores usually reduced to conidiogenous cells. Conidiogenous cells 5–10 × 2–5 μm (x̄ = 7.5 × 3 μm, n = 20), lining the pycnidial cavity, holoblastic, hyaline, cylindrical, discrete, determinate, smooth-walled. Conidia 14–18 × 6–8 μm (x̄ = 16 × 7 μm, n = 20), oblong to ovoid, straight, rounded at both ends, initially hyaline and aseptate becoming brown and 1-septate often while attached to conidiogenous cell, guttulate.
Culture characteristics: — Conidia germinating on PDA within 24 h. Germ tubes produced from one side of the conidium. Colonies on PDA reaching 1.0– 1.5 cm diam. after 10 days at 25 °C, circular, dense, convex with papillate surface, black colour in both upper and lower sides.
Material examined:— THAILAND. Phayao: on dead dry pod of Albizia lebbeck (Fabaceae), 20 July 2020, Achala Rathnayaka (MFLU 22-0093, holotype); ex-type living culture MFLUCC 22-0057.
Notes:—In the multi-gene phylogeny (ITS, LSU, tef 1-α and β-tub), our novel strain (MFLU 22-0093/ MFLUCC 22-0057) formed a separate lineage sister to D. thailandica with 81% ML bootstrap and 0.76 PP support (Fig. 1). Although the conidia of D. albiziae (16 × 7 μm) and D. thailandica (18.5 × 7 μm) are of similar size, there are several differences in conidial morphology between these two species (Phillips et al. 2013). Conidia in D. thailandica are oblong to ellipsoidal, slightly constricted at the septa, with slight undulating striations on the surface, occasionally curved and the lower cell is smaller than the upper cell (Phillips et al. 2013). In D. albiziae, the conidia are oblong to ovoid, not constricted at the septa, and the upper and lower cells are of similar size. Base pair differences (without gaps) between D. albiziae and D. thailandica are: ITS = 1.8% (8/455), LSU = 0.3% (2/794), tef 1-α = 3.7% (9/242) and β-tub = 2.1% (7/341). Considering the morpho-molecular data analysis, we established D. albiziae as a new species.
description: Published as part of Rathnayaka, Achala R., Chethana, K. W. Thilini, Phillips, Alan J. L. & Jones, E. B. Gareth, 2022, Two new species of Botryosphaeriaceae (Botryosphaeriales) and new host / geographical records, pp. 8-38 in Phytotaxa 564 (1) on page 19, DOI: 10.11646/phytotaxa.564.1.2, http://zenodo.org/record/7077775