title: ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð°ÐºÑÐ¸Ð½Ð¾Ð²Ð¾Ð³Ð¾ ÑÐ¸ÑÐ¾ÑÐºÐµÐ»ÐµÑÐ° ÑÐ¸Ð±ÑÐ¾Ð±Ð»Ð°ÑÑÐ¾Ð² Ð¿Ð¾Ð´ Ð´ÐµÐ¹ÑÑÐ²Ð¸ÐµÐ¼ ÐºÐ¾Ð»Ñ Ð¸ÑÐ¸Ð½Ð° Ð¼ÐµÑÐ¾Ð´Ð°Ð¼Ð¸ Ð°ÑÐ¾Ð¼Ð½Ð¾-ÑÐ¸Ð»Ð¾Ð²Ð¾Ð¹ Ð¸ ÐºÐ¾Ð½ÑÐ¾ÐºÐ°Ð»ÑÐ½Ð¾Ð¹ Ð»Ð°Ð·ÐµÑÐ½Ð¾Ð¹ ÑÐºÐ°Ð½Ð¸ÑÑÑÑÐµÐ¹ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: The study of change in the actin cytoskeleton of fibroblasts under the effect of colchicine by atomic force and confocal laser scanning microscopy
Creators: name: Ð Ð¾Ð·ÐµÐ½Ð±Ð»Ð¸Ñ, ÐÐ»Ð¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: Ð¿ÐµÑÐ²Ð¸ÑÐ½Ð°Ñ ÐºÑÐ»ÑÑÑÑÐ° ÑÐ¸Ð±ÑÐ¾Ð±Ð»Ð°ÑÑÐ¾Ð²
ÐºÐ¾Ð»Ñ Ð¸ÑÐ¸Ð½
ÑÐ¸ÑÐ¾ÑÐºÐµÐ»ÐµÑ
Ð°ÐºÑÐ¸Ð½
Ð°ÑÐ¾Ð¼Ð½Ð¾-ÑÐ¸Ð»Ð¾Ð²Ð°Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ
ÐºÐ¾Ð½ÑÐ¾ÐºÐ°Ð»ÑÐ½Ð°Ñ Ð»Ð°Ð·ÐµÑÐ½Ð°Ñ ÑÐºÐ°Ð½Ð¸ÑÑÑÑÐ°Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ
primary culture of fibroblasts
colchicine
cytoskeleton
actin
atomic force microscopy
confocal laser scanning microscopy

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description: The present work is devoted to a quantitative study of the effect of colchicine (1 Î¼g/ml) on the rigidity of fibroblasts and the structure of the actin skeleton. Tasks that were solved during the study: 1. Quantification of the change in the apparent Young's modulus of fibroblasts under the influence of colchicine using an atomic force microscope. 2. Quantification of the effect of colchicine on the change in the structure of the actin cytoskeleton of fibroblasts by confocal laser scanning microscopy. The results of atomic force microscopy show an increase in the apparent Young's modulus of the fibroblasts under the influence of colchicine by an average of â45%. The data obtained by laser scanning confocal microscopy demonstrate an increase in the fluorescence intensity of F-actin compared to control fibroblasts by an average of â40%. The results suggest that the destruction of microtubules by colchicine (1 Î¼g/ml) leads to a compensatory cell response, which manifests itself in an increase in actin content, which causes an increase in fibroblast rigidity. The approach used in this work can be used to quantify the molecular mechanism of action of drugs at the preclinical level of testing.