title: Ð¡ÑÐ°Ð²Ð½Ð¸ÑÐµÐ»ÑÐ½Ð¾Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ ÑÑÐ°Ð½ÑÐ¿Ð¾ÑÑÐ° Ð±ÐµÐ»ÐºÐ° IGFBP3 Ð² Ð½Ð°ÑÐ¸Ð²Ð½ÑÑ Ð¸ Ð½Ð¾ÐºÐ°ÑÑÐ½ÑÑ Ð¿Ð¾ Ð³ÐµÐ½Ñ Igfbp3 ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ð°Ð»ÑÐ½ÑÑ ÑÑÑÐ¾Ð¼Ð°Ð»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°.
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Transport comparison of IGFBP3 in native and Igfbp3 gene knockout human endometrial stromal cells
Creators: name: ÐÐ¸ÑÑÐµ, ÐÐ¸ÑÐ°Ð¸Ð»
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: ÐÐ»ÐµÑÐºÐ° (Ð±Ð¸Ð¾Ð».)
ÐÐµÐ»ÐºÐ¸
Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÐµ ÑÑÑÐ¾Ð¼Ð°Ð»ÑÐ½ÑÐµ ÐºÐ»ÐµÑÐºÐ¸ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°
Ð½Ð¾ÐºÐ°ÑÑ Ð³ÐµÐ½Ð°
ÑÐ½Ð´Ð¾ÑÐ¸ÑÐ¾Ð·
Ð²ÐµÐ·Ð¸ÐºÑÐ»ÑÑÐ½ÑÐ¹ ÑÑÐ°Ð½ÑÐ¿Ð¾ÑÑ
ÑÐ½Ð´Ð¾ÑÐ¾Ð¼Ñ
ÑÐ´ÐµÑÐ½Ð°Ñ ÑÑÐ°Ð½ÑÐ»Ð¾ÐºÐ°ÑÐ¸Ñ
human endometrial mesenchymal stromal cells
gene knockout
endocytosis
vesicular transport
endosomes
nuclear translocation

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐ¸ ÑÑÐ°Ð½ÑÐ¿Ð¾ÑÑÐ° ÑÐºÐ·Ð¾Ð³ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° IGFBP-3 Ð² Ð½Ð°ÑÐ¸Ð²Ð½ÑÑ Ð¼ÐµÐ·ÐµÐ½ÑÐ¸Ð¼Ð½ÑÑ ÑÑÑÐ¾Ð¼Ð°Ð»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ñ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°(ÑÐÐ¡Ð) Ð¸ ÐºÐ»ÐµÑÐºÐ°Ñ, Ð½Ð¾ÐºÐ°ÑÑÐ½ÑÑ Ð¿Ð¾ Ð³ÐµÐ½Ñ Ifbp3(ÐÐ-ÑÐÐ¡Ð). Ð Ð°Ð±Ð¾ÑÐ° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ ÐÐ½ÑÑÐ¸ÑÑÑÐ° ÑÐ¸ÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð Ð¾ÑÑÐ¸Ð¹ÑÐºÐ¾Ð¹ ÐÐºÐ°Ð´ÐµÐ¼Ð¸Ð¸ ÐÐ°ÑÐº. Ð¡ÑÐ°Ð²Ð½ÐµÐ½Ð¸Ðµ Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐ¸ ÑÑÐ°Ð½ÑÐ¿Ð¾ÑÑÐ° ÑÐºÐ·Ð¾Ð³ÐµÐ½Ð½Ð¾Ð³Ð¾ IGFBP-3 Ð² Ð½Ð°ÑÐ¸Ð²Ð½ÑÑ Ð¸ Ð½Ð¾ÐºÐ°ÑÑÐ½ÑÑ Ð¿Ð¾ Ð³ÐµÐ½Ñ Igfbp3 Ð¼ÐµÐ·ÐµÐ½ÑÐ¸Ð¼Ð½ÑÑ ÑÑÑÐ¾Ð¼Ð°Ð»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»Ð¾ÑÑ Ñ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸ÐµÐ¼ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾-Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð¸ ÑÐ¸ÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð¼ÐµÑÐ¾Ð´Ð¾Ð²: ÐÐºÑÐ°ÑÐºÐ° ÐºÐ»ÐµÑÐ¾Ðº3 ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾-Ð¼ÐµÑÐµÐ½Ð½ÑÐ¼Ð¸ Ð°Ð½ÑÐ¸ÑÐµÐ»Ð°Ð¼Ð¸ Ñ Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐµÐ¹ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÐ¾Ð¹ Ð¸Ð·Ð¾Ð±ÑÐ°Ð¶ÐµÐ½Ð¸Ð¹, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ ÐºÐ¾Ð½ÑÐ¾ÐºÐ°Ð»ÑÐ½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸, Ð¿Ð¾Ð·Ð²Ð¾Ð»Ð¸Ð»Ð° ÑÑÑÐ°Ð½Ð¾Ð²Ð¸ÑÑ Ð¾Ð±ÑÐ¸Ðµ Ð·Ð°ÐºÐ¾Ð½Ð¾Ð¼ÐµÑÐ½Ð¾ÑÑÐ¸ Ð¸ ÑÐ°Ð·Ð»Ð¸ÑÐ¸Ñ Ð² Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐµ ÑÐ½Ð´Ð¾ÑÐ¸ÑÐ¾Ð·Ð° Ð±ÐµÐ»ÐºÐ° Ð¸Ð½ÑÐµÑÐµÑÐ°. ÐÐµÑÐ¾Ð´Ð°Ð¼Ð¸ Ð±Ð¸Ð¾ÑÐ¸Ð¼Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÑÐ°ÐºÑÐ¸Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¸ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐµÐ³Ð¾ Ð²ÐµÑÑÐµÑÐ½-Ð±Ð»Ð¾Ñ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð±ÑÐ»Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ñ Ð´Ð°Ð½Ð½ÑÐµ Ð¾ Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐµ ÑÑÐ°Ð½ÑÐ¿Ð¾ÑÑÐ° ÑÐºÐ·Ð¾Ð³ÐµÐ½Ð½Ð¾Ð³Ð¾ IGFBP-3 Ð² ÑÐ´ÑÐ¾. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÑÑ ÑÐ´ÐµÐ»Ð°ÑÑ Ð¿ÑÐµÐ´Ð¿Ð¾Ð»Ð¾Ð¶ÐµÐ½Ð¸Ðµ Ð¾Ð± Ð¾ÑÑÑÑÑÑÐ²Ð¸Ð¸ Ð·Ð½Ð°ÑÐ¸ÑÐµÐ»ÑÐ½ÑÑ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹ Ð² Ð¿Ð°ÑÑÐµÑÐ½Ð°Ñ Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐ¸ ÑÐ½Ð´Ð¾ÑÐ¸ÑÐ¾Ð·Ð° ÑÐºÐ·Ð¾Ð³ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° Ð¸ ÐµÐ³Ð¾ ÑÑÐ°Ð½ÑÐ¿Ð¾ÑÑÐ° Ð² ÑÐ´ÑÐ¾ Ð² ÑÐ»ÑÑÐ°Ðµ Ð¾ÑÑÑÑÑÑÐ²Ð¸Ñ ÑÐ½Ð´Ð¾Ð³ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° Ð¸ Ð½Ð°Ð»Ð¸ÑÐ¸Ð¸ Ð½ÐµÐºÐ¾ÑÐ¾ÑÑÑ ÑÐ°Ð·Ð»Ð¸ÑÐ¸Ð¹ Ð² Ð¼Ð°ÑÐ¸Ð½ÐµÑÐ¸Ð¸ Ð´Ð°Ð½Ð½ÑÑ Ð¿ÑÐ¾ÑÐµÑÑÐ¾Ð².
description: The given work is devoted to studying the exogenous IGFBP-3 transport dynamics in native and Igfbp3gene knockout human endometrial mesenchymal stromal cells. The work was fulfilled on the premises of the Institute of Cytology of Russian Academy of Sciences. Exogenous IGFBP-3 transport dynamics comparison was performed via usage of various methods of molecular and cellular biology: immunofluorescent staining and confocal microscopy received images in different time spots and recombinant IGFBP-3 treatment approaches usage revealed common regularities and differences between protein endocytosis dynamics in native and knockout cells. Biochemical fractionation and western-blot analysis contributed to nuclear transport of IGFBP-3 investigation. Both experimental issues showed the lack of significant differences in exogenous IGFBP-3 transport dynamics patterns, but the distinction of these processesâ cellular machinery.