title: ÐÐ¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾-Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ°Ñ Ñ Ð°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ° ÑÑÐ°Ð¼Ð¼Ð° Ð²Ð¸ÑÑÑÐ° Ð³ÑÐ¸Ð¿Ð¿Ð° Ð/Leningrad/9/46/162/28 (H1N1)
title: Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÑÐºÐ°Ñ ÑÐ°Ð±Ð¾ÑÐ°
titleType: Subtitle
Creators: name: ÐÑÐ¸ÑÐµÐ½ÐºÐ¾, ÐÐµÑÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2016
Subjects: Ð²Ð¸ÑÑÑ Ð³ÑÐ¸Ð¿Ð¿Ð°
ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° ÑÑÐ°Ð¼Ð¼Ð°-Ð´Ð¾Ð½Ð¾ÑÐ°
Ð²ÑÑÐ¾ÐºÐ°Ñ ÑÐµÐ¿ÑÐ¾Ð´ÑÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ
Ñ Ð¾Ð»Ð¾Ð´Ð¾Ð²Ð°Ñ Ð°Ð´Ð°Ð¿ÑÐ°ÑÐ¸Ñ
ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÐ½Ð°Ñ ÑÑÐ²ÑÑÐ²Ð¸ÑÐµÐ»ÑÐ½Ð¾ÑÑÑ
influenza virus
properties of strain-donor
high-yield
cold adaption
temperature sensitive

description: Ð¦ÐµÐ»ÑÑ Ð½Ð°ÑÑÐ¾ÑÑÐµÐ¹ ÑÐ°Ð±Ð¾ÑÑ ÑÑÐ°Ð»Ð° ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ° ÑÑÐ°Ð¼Ð¼Ð° Ð²Ð¸ÑÑÑÐ° Ð³ÑÐ¸Ð¿Ð¿Ð° A/Leningrad/9/46/162/28 (H1N1). Ð­ÑÐ¾Ñ ÑÑÐ°Ð¼Ð¼ Ð±ÑÐ» Ð¿Ð¾Ð»ÑÑÐµÐ½ Ð¸Ð· ÑÐ¿Ð¸Ð´ÐµÐ¼Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÑÐ°Ð¼Ð¼Ð° A/Leningrad/9/1946 (H1N1) Ð² Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ Ð³ÑÐ¸Ð¿Ð¿Ð¾Ð·Ð½ÑÑ Ð²Ð°ÐºÑÐ¸Ð½ Ð¤ÐÐÐ£ Â«ÐÐÐ Ð³ÑÐ¸Ð¿Ð¿Ð°Â» Ð¿Ð¾ÑÑÐµÐ´ÑÑÐ²Ð¾Ð¼ Ð´Ð»Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ Ð¿Ð°ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð² ÑÐ¸ÑÑÐµÐ¼Ðµ ÑÐ°Ð·Ð²Ð¸Ð²Ð°ÑÑÐ¸ÑÑÑ ÐºÑÑÐ¸Ð½ÑÑ ÑÐ¼Ð±ÑÐ¸Ð¾Ð½Ð¾Ð² Ð¿ÑÐ¸ Ð¾Ð¿ÑÐ¸Ð¼Ð°Ð»ÑÐ½Ð¾Ð¹ Ð¸ Ð¿Ð¾Ð½Ð¸Ð¶ÐµÐ½Ð½Ð¾Ð¹ ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÐ°Ñ. ÐÐ½ Ð±ÑÐ» Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½ Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð´Ð¾Ð½Ð¾ÑÐ° Ð²ÑÑÐ¾ÐºÐ¾Ð¹ ÑÐµÐ¿ÑÐ¾Ð´ÑÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð¿ÑÐ¸ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐµ ÑÐµÐ·Ð¾Ð½Ð½Ð¾Ð¹ Ð¸Ð½Ð°ÐºÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð¹ Ð³ÑÐ¸Ð¿Ð¿Ð¾Ð·Ð½Ð¾Ð¹ Ð²Ð°ÐºÑÐ¸Ð½Ñ Ð¿Ð¾Ð´ÑÐ¸Ð¿Ð° H3N2 Ð² 1989 Ð³Ð¾Ð´Ñ. ÐÐ¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ ÑÑÐ°Ð¼Ð¼ A/Leningrad/9/46/162/28 (H1N1) Ð¾Ð±Ð»Ð°Ð´Ð°ÐµÑ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð¾Ð¼ Ð²ÑÑÐ¾ÐºÐ¾Ð¹ ÑÐµÐ¿ÑÐ¾Ð´ÑÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð² ÑÐ¸ÑÑÐµÐ¼Ðµ ÑÐ°Ð·Ð²Ð¸Ð²Ð°ÑÑÐ¸ÑÑÑ ÐºÑÑÐ¸Ð½ÑÑ ÑÐ¼Ð±ÑÐ¸Ð¾Ð½Ð¾Ð² Ð¸ Ð² ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ ÐºÑÐ»ÑÑÑÑÐµ MDCK, Ð° ÑÐ°ÐºÐ¶Ðµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð°Ð¼Ð¸ ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÐ¾ÑÑÐ²ÑÑÐ²Ð¸ÑÐµÐ»ÑÐ½Ð¾ÑÑÐ¸ Ð¸ ÑÐ¾Ð»Ð¾Ð´Ð¾Ð°Ð´Ð°Ð¿ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾ÑÑÐ¸. ÐÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¾ Ð¿Ð¾Ð»Ð½Ð¾Ð³ÐµÐ½Ð¾Ð¼Ð½Ð¾Ðµ ÑÐµÐºÐ²ÐµÐ½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ, Ð²ÑÑÐ²Ð¸Ð²ÑÐµÐµ Ð¾Ð¿Ð¸ÑÐ°Ð½Ð½ÑÐ¹ Ð² Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐµ ÑÐ°ÐºÑÐ¾Ñ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ñ Ð¿Ð°ÑÐ¾Ð³ÐµÐ½Ð½Ð¾ÑÑÐ¸, Ð° ÑÐ°ÐºÐ¶Ðµ ÑÑÐ´ Ð¼ÑÑÐ°ÑÐ¸Ð¹ Ð¸ Ð¿Ð¾Ð»Ð¸Ð¼Ð¾ÑÑÐ¸Ð·Ð¼Ð¾Ð², Ð¾ÑÐ²ÐµÑÑÑÐ²ÐµÐ½Ð½ÑÑ Ð·Ð° Ð¿ÑÐ¸ÑÐ¿Ð¾ÑÐ¾Ð±Ð»ÑÐµÐ¼Ð¾ÑÑÑ ÑÑÐ°Ð¼Ð¼Ð° A/Leningrad/9/46/162/28 (H1N1) Ðº ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÐ¼ ÑÐ¸ÑÑÐµÐ¼Ð°Ð¼ Ð¸ ÑÑÐ»Ð¾Ð²Ð¸ÑÐ¼ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ. Ð¡Ð¾Ð²Ð¾ÐºÑÐ¿Ð½Ð¾ÑÑÑ ÑÑÐ¸Ñ ÑÐ²Ð¾Ð¹ÑÑÐ² ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸Ð·ÑÐµÑ ÑÑÐ¾Ñ ÑÑÐ°Ð¼Ð¼ ÐºÐ°Ðº ÑÐ½Ð¸Ð²ÐµÑÑÐ°Ð»ÑÐ½ÑÐ¹ Ð´Ð¾Ð½Ð¾Ñ Ð´Ð»Ñ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´ÑÑÐ²Ð° Ð³ÑÐ¸Ð¿Ð¿Ð¾Ð·Ð½ÑÑ Ð²Ð°ÐºÑÐ¸Ð½.
description: The aim of this work was to characterize influenza A virus strain A/Leningrad/9/46/162/28 (H1N1). This strain was obtained from epidemic strain A/Leningrad/9/1946 (H1N1) in the Laboratory of Influenza Vaccines via multiple passages in the system of developing chicken embryos at optimal and reduced temperatures. It was once used as a high-yield donor while developing seasonal H3N2 influenza vaccine in 1989. It is shown that A/Leningrad/9/46/162/28 (H1N1) strain possesses properties of high-yield in the system of developing chicken embryos and MDCK cell culture, as well as properties of cold adaptaion and temperature sensitivity. Whole genome next generation sequencing showed described in the literature attenuation factor and several mutations and polymorphisms that are obviously responsible for strains adaptation to different cultivation systems and conditions. This set of properties characterize the influenza virus A/Leningrad/9/46/162/28 (H1N1) strain as a potential universal donor for both inactivated and live influenza vaccines.