Additional file 2 of Parasite-host ecology: the limited impacts of an intimate enemy on host microbiomes
Creators: name: Clements, Cody S.
name: Burns, Andrew S.
name: Stewart, Frank J.
name: Hay, Mark E.
Publisher: name: figshare
Year: 2020
Subjects: subject: Microbiology
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Ecology

description: Additional file 2 Fig. S1. (a) Coralliophila violacea (red arrows) feeding on natural colonies of Porites cylindrica in the field. (b) C. violacea feeding scars (red arrows) on natural P. cylindrica colonies. (c) Experimental set-up assessing the effects of C. violacea feeding on P. cylindrica growth and survivorship. (d) C. violacea feeding scars (red arrows) at the end of the 24 day experiment. Fig. S2: ESV rarefaction curves for each of the 76 coral samples we evaluated. The vertical line indicates sampling depth (8013 sequences). Fig. S3: (a) Sampling schematic of basal locations (using rarefied data) from corals in our manipulative experiment and natural colonies in the field used for analyses (one-factor test, factor: treatment). (b) Microbiome composition (beta diversity) of basal samples among treatments. (c) Microbiome variability (beta dispersion) of basal samples among treatments. (d) Microbiome alpha diversity of basal samples among treatments. Analyses were performed following rarefaction (subsampling without replacement) to a uniform sequence count of 8013 sequences per sample. Fig. S4: (a) Sampling schematic of basal locations (using non-rarefied data) from corals in our manipulative experiment and natural colonies in the field used for analyses (one-factor test, factor: treatment). (b) Microbiome composition (beta diversity) of basal samples among treatments. (c) Microbiome variability (beta dispersion) of basal samples among treatments. (d) Microbiome alpha diversity of basal samples among treatments. Fig. S5: (a) Sampling schematic of distal locations (using rarefied data) from corals in our manipulative experiment and natural colonies in the field used for analyses (one-factor test, factor: treatment). (b) Microbiome composition (beta diversity) of distal samples among treatments. (c) Microbiome variability (beta dispersion) of distal samples among treatments. (d) Microbiome alpha diversity of distal samples among treatments. Analyses were performed following rarefaction (subsampling without replacement) to a uniform sequence count of 8013 sequences per sample. Fig. S6: (a) Sampling schematic of distal locations (using non-rarefied data) from corals in our manipulative experiment and natural colonies in the field used for analyses (one-factor test, factor: treatment). (b) Microbiome composition (beta diversity) of distal samples among treatments. (c) Microbiome variability (beta dispersion) of distal samples among treatments. (d) Microbiome alpha diversity of distal samples among treatments. Analyses were performed using a non-rarefied dataset. Fig. S7: (a) Sampling schematic of scar locations (using non-rarefied data) from corals in our manipulative experiment and natural colonies in the field used for analyses (one-factor test, factor: treatment). (b) Microbiome composition (beta diversity) of distal samples among treatments. (c) Microbiome variability (beta dispersion) of distal samples among treatments. (d) Microbiome alpha diversity of distal samples among treatments. Fig. S8: (a) Sampling schematic of locations (using non-rarefied data) from corals in our manipulative experiment that were subjected to snail feeding used for analyses (one-factor test, factor: location). (b) Microbiome composition (beta diversity) of samples by location. Letters in legend denote significant differences (p 2) for those ESVs that were greater than 0.5% of the community in either scar, basal, or distal locations on coral outplants fed on by snails. Most ESVs could be identified to the genus or family level (right). Letters indicate significant groupings (FDR-adjusted p 2) for those ESVs that were greater than 0.5% of the community in either scar, basal, or distal locations on natural colonies fed on by snails. Most ESVs could be identified to the genus or family level (right). Letters indicate significant groupings (FDR-adjusted p