title: ÐÐ½Ð°Ð»Ð¸Ð· Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð¼Ð¾Ð´ÐµÐ»Ð¸ÑÑÐµÐ¼Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾Ð³ÑÐ°Ð²Ð¸ÑÐ°ÑÐ¸Ð¸ Ð½Ð° Ð´Ð¾ÑÐ°Ð¼Ð¸Ð½ÐµÑÐ³Ð¸ÑÐµÑÐºÑÑ ÑÐ¸ÑÑÐµÐ¼Ñ ÑÑÑÐ¸Ð°ÑÑÐ¼Ð° Ñ Ð¼ÑÑÐµÐ¹
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Analysis of the effect of simulated microgravity on the striatal dopaminergic system in mice
Creators: name: Ð¥ÑÐ°Ð¼ÑÐ¾Ð²Ð°, ÐÐ½Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Ð¼Ð¸ÐºÑÐ¾Ð³ÑÐ°Ð²Ð¸ÑÐ°ÑÐ¸Ñ
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ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸Ð¾Ð½Ð½ÑÐ¹ ÑÐ°ÐºÑÐ¾Ñ CREB
microgravity
hindlimb unloading
dorsal striatum
tyrosine hydroxylase
dopamine receptors
protein kinase A
CREB transcription factor

description: ÐÐ·Ð²ÐµÑÑÐ½Ð¾, ÑÑÐ¾ ÐºÐ¾ÑÐ¼Ð¸ÑÐµÑÐºÐ¸Ðµ Ð¿Ð¾Ð»ÑÑÑ Ð¸ Ð¼Ð¸ÐºÑÐ¾Ð³ÑÐ°Ð²Ð¸ÑÐ°ÑÐ¸Ñ Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÑÑÐ·Ð½Ð°ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð½Ð° ÑÐµÐ½ÑÑÐ°Ð»ÑÐ½ÑÑ Ð½ÐµÑÐ²Ð½ÑÑ ÑÐ¸ÑÑÐµÐ¼Ñ. ÐÐ´Ð½Ð°ÐºÐ¾ Ð²Ð¾Ð¿ÑÐ¾Ñ Ð¾ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð°Ð»ÑÐ½Ð¾Ð¼ ÑÐ¾ÑÑÐ¾ÑÐ½Ð¸Ð¸ Ð´Ð¾ÑÐ°Ð¼Ð¸Ð½ÐµÑÐ³Ð¸ÑÐµÑÐºÐ¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ñ ÑÑÑÐ¸Ð°ÑÑÐ¼Ð° Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑÐ¼Ð¸ÐºÑÐ¾Ð³ÑÐ°Ð²Ð¸ÑÐ°ÑÐ¸Ð¸ Ð¾ÑÑÐ°ÐµÑÑÑ Ð¾ÑÐºÑÑÑÑÐ¼. Ð Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ Ð¼Ñ Ð¸Ð·ÑÑÐ¸Ð»Ð¸ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ 3-Ð´Ð½ÐµÐ²Ð½Ð¾Ð¹ ÑÐ¸Ð¼ÑÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾Ð³ÑÐ°Ð²Ð¸ÑÐ°ÑÐ¸Ð¸ Ð½Ð° ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ðµ Ð¸ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑÑÐµÑÐ¼ÐµÐ½ÑÐ° ÑÐ¸ÑÐ¾Ð·Ð¸Ð½Ð³Ð¸Ð´ÑÐ¾ÐºÑÐ¸Ð»Ð°Ð·Ñ, ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ðµ Ð´Ð¾ÑÐ°Ð¼Ð¸Ð½Ð¾Ð²ÑÑ ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð² D1 Ð¸D2, Ð° ÑÐ°ÐºÐ¶Ðµ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð¿ÑÐ¾ÑÐµÐ¸Ð½ÐºÐ¸Ð½Ð°Ð·Ñ Ð Ð¸ ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸Ð¾Ð½Ð½Ð¾Ð³Ð¾ ÑÐ°ÐºÑÐ¾ÑÐ° CREBÐ² Ð´Ð¾ÑÑÐ°Ð»ÑÐ½Ð¾Ð¼ ÑÑÑÐ¸Ð°ÑÑÐ¼Ðµ Ð¼ÑÑÐµÐ¹. ÐÐ»Ñ Ð¼Ð¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð½ÐµÐ²ÐµÑÐ¾Ð¼Ð¾ÑÑÐ¸ Ð¼ÑÐ¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ Ð¼Ð¾Ð´ÐµÐ»Ñ Ð°Ð½ÑÐ¸Ð¾ÑÑÐ¾ÑÑÐ°ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ ÑÐ°Ð·Ð³ÑÑÐ·ÐºÐ¸ Ð½Ð¸Ð¶Ð½Ð¸Ñ ÐºÐ¾Ð½ÐµÑÐ½Ð¾ÑÑÐµÐ¹(Ð¼Ð¾Ð´ÐµÐ»Ñ Ð²ÑÐ²ÐµÑÐ¸Ð²Ð°Ð½Ð¸Ñ) Ð¿Ð¾ ÐÐ¾Ð²Ð¸ÐºÐ¾Ð²Ñ-ÐÐ»ÑÐ¸Ð½Ñ Ð² Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Morey-Holton.Ð¡Ð¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ðµ Ð¸ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð±ÐµÐ»ÐºÐ¾Ð² Ð¸Ð½ÑÐµÑÐµÑÐ° Ð¸Ð·ÑÑÐ°Ð»Ð¸ Ð¿ÑÐ¸ Ð¿Ð¾Ð¼Ð¾ÑÐ¸Ð¸Ð¼Ð¼ÑÐ½Ð¾Ð³Ð¸ÑÑÐ¾ÑÐ¸Ð¼Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¸ ÐÐµÑÑÐµÑÐ½-Ð±Ð»Ð¾Ñ Ð°Ð½Ð°Ð»Ð¸Ð·Ð°. ÐÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ 3-Ð´Ð½ÐµÐ²Ð½Ð°Ñ ÑÐ°Ð·Ð³ÑÑÐ·ÐºÐ° Ð·Ð°Ð´Ð½Ð¸Ñ ÐºÐ¾Ð½ÐµÑÐ½Ð¾ÑÑÐµÐ¹ Ð¿ÑÐ¸Ð²Ð¾Ð´Ð¸Ñ Ðº ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ñ ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸ÑÑÐ¸ÑÐ¾Ð·Ð¸Ð½Ð³Ð¸Ð´ÑÐ¾ÐºÑÐ¸Ð»Ð°Ð·Ñ Ð² Ð´Ð¾ÑÑÐ°Ð»ÑÐ½Ð¾Ð¼ ÑÑÑÐ¸Ð°ÑÑÐ¼Ðµ, ÑÑÐ¾ ÑÐ²Ð¸Ð´ÐµÑÐµÐ»ÑÑÑÐ²ÑÐµÑ Ð¾ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ð¸Ð±Ð¸Ð¾ÑÐ¸Ð½ÑÐµÐ·Ð° Ð´Ð¾ÑÐ°Ð¼Ð¸Ð½Ð°. ÐÑÐ¸ ÑÑÐ¾Ð¼ Ð¿ÑÐ¾Ð¸ÑÑÐ¾Ð´Ð¸Ñ Ð¿Ð¾Ð²ÑÑÐµÐ½Ð¸Ðµ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ÑÐ¸ÑÐ¾Ð·Ð¸Ð½Ð³Ð¸Ð´ÑÐ¾ÐºÑÐ¸Ð»Ð°Ð·Ñ, ÐºÐ¾ÑÐ¾ÑÐ¾Ðµ Ð¼Ð¾Ð¶ÐµÑ Ð¸Ð³ÑÐ°ÑÑ ÐºÐ¾Ð¼Ð¿ÐµÐ½ÑÐ°ÑÐ¾ÑÐ½ÑÑ ÑÐ¾Ð»Ñ. ÐÑÐ¾Ð¼ÐµÑÐ¾Ð³Ð¾, Ð¿Ð¾ÑÐ»Ðµ 3-Ð´Ð½ÐµÐ²Ð½Ð¾Ð³Ð¾ Ð²ÑÐ²ÐµÑÐ¸Ð²Ð°Ð½Ð¸Ñ Ð½Ð°Ð±Ð»ÑÐ´Ð°ÐµÑÑÑ Ð¿Ð¾Ð²ÑÑÐµÐ½Ð¸Ðµ ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸ÑÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð² D1 Ð² Ð´Ð¾ÑÑÐ°Ð»ÑÐ½Ð¾Ð¼ ÑÑÑÐ¸Ð°ÑÑÐ¼Ðµ, Ð² ÑÐ¾ Ð²ÑÐµÐ¼Ñ ÐºÐ°Ðº ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ðµ ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð²D2 Ð½Ðµ Ð¸Ð·Ð¼ÐµÐ½ÑÐµÑÑÑ. Ð¢Ð°ÐºÐ¶Ðµ Ð²ÑÑÐ²Ð»ÐµÐ½Ð¾ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ðµ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ CREB Ð¸Ð¿ÑÐ¾ÑÐµÐ¸Ð½ÐºÐ¸Ð½Ð°Ð·Ñ Ð. Ð ÑÐ¾Ð²Ð¾ÐºÑÐ¿Ð½Ð¾ÑÑÐ¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÐµ Ð´Ð°Ð½Ð½ÑÐµ ÑÐ²Ð¸Ð´ÐµÑÐµÐ»ÑÑÑÐ²ÑÑÑ Ð¾Ð½Ð°ÑÑÑÐµÐ½Ð¸Ð¸ Ð´Ð¾ÑÐ°Ð¼Ð¸Ð½ÐµÑÐ³Ð¸ÑÐµÑÐºÐ¾Ð¹ ÑÐµÐ³ÑÐ»ÑÑÐ¸Ð¸ ÑÐ°Ð±Ð¾ÑÑ Ð´Ð¾ÑÑÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÑÑÐ¸Ð°ÑÑÐ¼Ð°Ð¿Ð¾ÑÐ»Ðµ 3-Ð´Ð½ÐµÐ²Ð½Ð¾Ð³Ð¾ Ð°Ð½ÑÐ¸Ð¾ÑÑÐ¾ÑÑÐ°ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð²ÑÐ²ÐµÑÐ¸Ð²Ð°Ð½Ð¸Ñ.
description: It is known that spaceflight and microgravity have a significant effect on thecentral nervous system. However, the question about the functional state of thedopaminergic system of the striatum under microgravity conditions remains open.Here we analyzed the effect of 3-day simulated microgravity on the content andactivity of the enzyme tyrosine hydroxylase, the content of dopamine receptors D1and D2, as well as the activity of protein kinase A and the transcription factor CREBin the dorsal striatum of mice. To simulate microgravity, we used the hindlimbunloading rodent model by Novikov-Ilyin (tail suspension model) modified byMorey-Holton. The content and activity of proteins of interest were analyzed byimmunohistochemical and Western blot analysis. We showed that 3-day hindlimbunloading resulted in a decrease in tyrosine hydroxylase content in the dorsalstriatum, indicating a decrease in dopamine biosynthesis. At the same time, anincrease in tyrosine hydroxylase activity was detected, which may play acompensatory role. In addition, after 3 days of unloading, we revealedan increase inthe content of D1 receptors in the dorsal striatum, while the content of D2 receptorsdid not change. Decreased activity of CREB and protein kinase A was also observed.Thus, our data demonstrate the impairment of dopaminergic regulation in the dorsalstriatum after 3-day hindlimb unloading.