Associations of vitamin D binding protein variants with the vitamin D-induced increase in serum 25-hydroxyvitamin D
Creators: name: Hanachi, Parichehr
name: Mehrane Mehramiz
name: Sayyed Saeid Khayyatzadeh
name: Esmaily, Habibollah
name: Faezeh Ghasemi
name: Ardekani, Kiana Sadeghi
name: Tayefi, Maryam
name: Mirmousavi, Seyed Jamal
name: H. Bahrami-Taghanaki
name: Eslamii, Saeed
name: Vatanparast, Hasan
name: A.Ferns, Gordon
name: Ghayour-Mobarhan, Majid
name: Avan, Amir
Publisher: name: figshare
Year: 2021
Subjects: subject: Biochemistry
subject: 60199 Biochemistry and Cell Biology not elsewhere classified
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: 60101 Analytical Biochemistry
subject: 110199 Medical Biochemistry and Metabolomics not elsewhere classified
subject: FOS: Clinical medicine
subject: FOS: Clinical medicine
subject: Biotechnology
subject: Biological Techniques
subject: 69999 Biological Sciences not elsewhere classified
subject: 60114 Systems Biology
subject: 111201 Cancer Cell Biology
subject: Molecular Biology
subject: Cell Biology
subject: 119999 Medical and Health Sciences not elsewhere classified
subject: FOS: Other medical sciences
subject: FOS: Other medical sciences

description: AbstractBackground: Vitamin D deficiency is a global problem that may be improved by vitamin D supplementation; however, the individual's response to the intervention varies. We aimed to investigate possible genetic factors that may modify the impact of environmental exposure on vitamin D status. The candidate gene variant we investigated was the Gc gene-rs4588 polymorphism at the vitamin D receptor (DBP) locus. Methods: A total of 619 healthy adolescent Iranian girls received 50000 IU of vitamin D 3 weekly for 9 weeks. Serum 25(OH) D concentrations, metabolic profiles and dietary intake were measured at baseline and after 9 weeks of supplementation. The genotypes of the DBP variant (rs4588) were analyzed using the TaqMan genotyping assay. Results: Our results revealed that the rs4588 polymorphism might be associated with serum 25-hydroxy vitamin D both at baseline (p value = 0.03) and after intervention (p value = 0.008). It seemed that the outcome of the intervention was gene-related so that the subjects with common AA genotype were a better responder to vitamin D supplementation (Changes (%) 469.5 (427.1) in AA carriers vs. 335.8 (530) in GG holders), and carriers of the less common GG genotype experienced a rise in fasting blood glucose after 9 weeks (Changes (%) 0 (1.5)). Our findings also showed that the statistical interaction between this variant and supplementation was statistically significant (intervention effect p-value&lt;0.001 and p-value SNP effect = 0.03). The regression model also revealed that after adjusted for potential confounders, likelihood of affecting serum 25(OH)D in individuals who were homozygous for the uncommon allele G was less than those homozygous for the more common AA genotype (OR = 4.407 (1.82–8.89); p = 0.001). Conclusion: Serum vitamin 25(OH) D following vitamin 25(OH) D 3 supplementation appears to be modified by genetic background. The Gc genetic variant, rs4588 encoding the vitamin D receptor seems to influence the response to vitamin D supplementation.