Additional file 1 of Neuroendocrine patterns underlying seasonal song and year-round territoriality in male black redstarts
Creators: name: Villavicencio, Camila P.
name: Windley, Harriet
name: D’Amelio, Pietro B.
name: Gahr, Manfred
name: Goymann, Wolfgang
name: Quispe, René
Publisher: name: figshare
Year: 2021
Subjects: subject: Biochemistry
subject: Genetics
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Neuroscience
subject: Physiology
subject: Evolutionary Biology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: FOS: Chemical sciences
subject: Ecology
subject: Cancer
subject: 111714 Mental Health
subject: FOS: Health sciences
subject: FOS: Health sciences

description: Additional file 1: Supplementary Figure 1: Pearson correlation coefficients and their 95% confidence intervals as a measure of standardized effect sizes of all aggressive traits measured in relation to testosterone concentrations. Positive coefficients indicate positive association of the respective behaviour with testosterone. All effects were slightly positive, but the 95% confidence intervals included zero, suggesting that the degree of aggressiveness was not strongly associated testosterone (t5m: time in 5-m radius around the decoy, lat: latency to approach the decoy, nod: frequency of head nodding, ca: closest approach to decoy, song: song rate). To be consistent with the other behaviors, the latency to approach and the closest approach are represented with a negative sign, because smaller latencies and closer approaches indicate higher aggression, whereas for all other behaviors larger values indicate higher aggression. Supplementary Figure 2: Pearson correlation coefficients (± 95% confidence intervals) of all aggressive behavioral traits measured in relation to aromatase, androgen receptor and estrogen receptor expression in the preoptic area (POA, top row panels) and hypothalamic areas (H, lower row panels). Correlation coefficients were consistently positive for aggressive behaviours in relation to aromatase expression in the hypothalamus, but the 95% confidence intervals included zero in all of the measures, suggesting that there was no strong relationship between aggression and aromatase expression in the hypothalamus. The correlation coefficients of all other measures in both brain areas included negative and positive values suggesting no consistent association with aggressive behavior (see Supplementary Fig. 1 for further information). Supplementary Figure 3. Correlation between territorial behaviors in response to an STI and brain aromatase expression in the preoptic area and estrogen receptor expression in the hypothalamus. The nodding frequency correlated positively with the expression of aromatase in the preoptic area when corrected for stage (Faro=7.33, Fstage = 2.36, p = 0.02, R2 = 0.25; Suppl. Fig. 3A). The time spent in a 5-m radius around the decoy was negatively correlated with the expression of estrogen receptors (Fer=6.84, Fstage = 3.13, p = 0.02, R2 = 0.4) and the closest approach was positively related to the expression of estrogen receptors (Fer=6.19, Fstage = 0.8, p = 0.03, R2 = 0.5) in the hypothalamus (Suppl. Fig. 3B). Supplementary Figure 4: Song rate in relation to brain sensitivity. The song frequency was positively related to the optical density of AR in lMan (deviance= 154.33 p