title: ÐÑÐ¾Ð±ÐµÐ½Ð½Ð¾ÑÑÐ¸ ÑÑÑÐµÑÑ-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Ð¸Ð¾Ð½Ð¾ÑÑÐ¾Ð¿Ð½ÑÑ Ð³Ð»ÑÑÐ°Ð¼Ð°ÑÐ½ÑÑ ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð² Ð² Ð¼Ð¾Ð·Ð³Ðµ Ð²Ð·ÑÐ¾ÑÐ»ÑÑ ÐºÑÑÑ Ð¿Ð¾ÑÐ»Ðµ Ð½ÐµÐ¾Ð½Ð°ÑÐ°Ð»ÑÐ½ÑÑ Ð²Ð²ÐµÐ´ÐµÐ½Ð¸Ð¹ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÐ½Ð´Ð¾ÑÐ¾ÐºÑÐ¸Ð½Ð°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Effects of neonatal administration of bacterial endotoxin on stress-induced changes in the expression of ionotropic glutamate receptor genes in the brain of adult rats
Creators: name: ÐÐ¸ÐºÐ¸ÑÐ¸Ð½Ð°, ÐÐµÑÐ¾Ð½Ð¸ÐºÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: Ð¿ÐµÑÐ¸Ð½Ð°ÑÐ°Ð»ÑÐ½ÑÐ¹ Ð¿ÐµÑÐ¸Ð¾Ð´
Ð²Ð¾ÑÐ¿Ð°Ð»ÐµÐ½Ð¸Ðµ
Ð»Ð¸Ð¿Ð¾Ð¿Ð¾Ð»Ð¸ÑÐ°Ñ Ð°ÑÐ¸Ð´
ÑÑÑÐµÑÑ
ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ðµ Ð¼Ð¾Ð·Ð³Ð°
NMDA-ÑÐµÑÐµÐ¿ÑÐ¾Ñ
AMPA-ÑÐµÑÐµÐ¿ÑÐ¾Ñ
Ð¿Ð¾Ð²ÐµÐ´ÐµÐ½Ð¸Ðµ
Ð´Ð¾ÑÐ·Ð°Ð»ÑÐ½ÑÐ¹ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿
Ð²ÐµÐ½ÑÑÐ°Ð»ÑÐ½ÑÐ¹ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿
Ð¼ÐµÐ´Ð¸Ð°Ð»ÑÐ½Ð°Ñ Ð¿ÑÐµÑÑÐ¾Ð½ÑÐ°Ð»ÑÐ½Ð°Ñ ÐºÐ¾ÑÐ°
Ð°Ð¼Ð¸Ð³Ð´Ð°Ð»Ð°
perinatal period
inflammation
lipopolysaccharide
stress
brain development
NMDA receptor
AMPA-receptor
behavior
dosrsal hippocampus
ventral hippocampus
medial prefrontal cortex
amygdala

description: Ð Ð°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ ÑÐ°Ð·Ð»Ð¸ÑÐ¸Ð¹ Ð² ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Ð¾ÑÐ´ÐµÐ»ÑÐ½ÑÑ ÑÑÐ±ÑÐµÐ´Ð¸Ð½Ð¸Ñ NMDA-ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð² (GluN1, GluN2A, GluN2B) Ð¸ AMPA-ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð² (GluA1, GluA2) Ð² Ð¼ÐµÐ´Ð¸Ð°Ð»ÑÐ½Ð¾Ð¹ Ð¿ÑÐµÑÑÐ¾Ð½ÑÐ°Ð»ÑÐ½Ð¾Ð¹ ÐºÐ¾ÑÐµ, Ð²ÐµÐ½ÑÑÐ°Ð»ÑÐ½Ð¾Ð¼ Ð¸ Ð´Ð¾ÑÐ·Ð°Ð»ÑÐ½Ð¾Ð¼ Ð¾ÑÐ´ÐµÐ»Ð°Ñ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð°, Ð°Ð¼Ð¸Ð³Ð´Ð°Ð»Ðµ ÐºÑÑÑ, Ð¿Ð¾Ð»ÑÑÐ°Ð²ÑÐ¸Ñ Ð²Ð²ÐµÐ´ÐµÐ½Ð¸Ñ Ð»Ð¸Ð¿Ð¾Ð¿Ð¾Ð»Ð¸ÑÐ°ÑÐ°ÑÐ¸Ð´Ð° (ÐÐÐ¡) Ð² ÑÐµÑÐµÐ½Ð¸Ðµ 3-Ð¹ Ð½ÐµÐ´ÐµÐ»Ð¸ Ð¶Ð¸Ð·Ð½Ð¸, Ð² Ð¾Ð±ÑÑÐ½ÑÑ ÑÑÐ»Ð¾Ð²Ð¸ÑÑ Ð¸ ÑÐµÑÐµÐ· 25 Ð´Ð½ÐµÐ¹ Ð¿Ð¾ÑÐ»Ðµ Ð²Ð¸ÑÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÑÑÐµÑÑÐ°, Ð° ÑÐ°ÐºÐ¶Ðµ Ð°Ð½Ð°Ð»Ð¸Ð·Ñ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹ Ð¿Ð¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ Ñ ÑÑÐ¸Ñ Ð¶Ð¸Ð²Ð¾ÑÐ½ÑÑ. Ð Ð°Ð±Ð¾ÑÐ° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð½Ð° ÐºÑÑÑÐ°Ñ-ÑÐ°Ð¼ÑÐ°Ñ ÐÐ¸ÑÑÐ°Ñ. Ð£ÑÐ¾Ð²ÐµÐ½Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Ð¾ÑÐµÐ½Ð¸Ð²Ð°Ð»Ð¸ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ ÐÐ¢-ÐÐ¦Ð  Ð² ÑÐµÐ°Ð»ÑÐ½Ð¾Ð¼ Ð²ÑÐµÐ¼ÐµÐ½Ð¸, Ð¾ÑÐµÐ½ÐºÑ Ð¿Ð¾ÐºÐ°Ð·Ð°ÑÐµÐ»ÐµÐ¹ ÑÑÐµÐ²Ð¾Ð¶Ð½Ð¾ÑÑÐ¸ Ð¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÑÐºÐ¾Ð³Ð¾ Ð¿Ð¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»Ð¸ Ð² ÑÐµÑÑÐ°Ñ Â«ÐÑÐºÑÑÑÐ¾Ðµ Ð¿Ð¾Ð»ÐµÂ» Ð¸ Â«ÐÑÐ¸Ð¿Ð¾Ð´Ð½ÑÑÑÐ¹ ÐºÑÐµÑÑÐ¾Ð¾Ð±ÑÐ°Ð·Ð½ÑÐ¹ Ð»Ð°Ð±Ð¸ÑÐ¸Ð½ÑÂ». ÐÐ²ÐµÐ´ÐµÐ½Ð¸Ðµ ÐÐÐ¡ Ð² ÑÐµÑÐµÐ½Ð¸Ðµ 3-Ð¹ Ð½ÐµÐ´ÐµÐ»Ð¸ Ð¶Ð¸Ð·Ð½Ð¸ Ð²ÑÐ·ÑÐ²Ð°Ð»Ð¾ Ñ Ð²Ð·ÑÐ¾ÑÐ»ÑÑ ÐºÑÑÑ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ðµ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² ÑÑÐ±ÑÐµÐ´Ð¸Ð½Ð¸Ñ GluA1, GluA2, GluN2A, GluN2B Ð² Ð´Ð¾ÑÐ·Ð°Ð»ÑÐ½Ð¾Ð¼ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ðµ Ð¸ GluA1 Ð¸ GluA2 â Ð² Ð²ÐµÐ½ÑÑÐ°Ð»ÑÐ½Ð¾Ð¼. Ð Ð°Ð·Ð»Ð¸ÑÐ¸Ñ Ð² ÑÐµÐ°ÐºÑÐ¸Ð¸ Ð½Ð° ÑÑÑÐµÑÑ Ñ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½ÑÑ Ð¸ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ ÐºÑÑÑ Ð²ÑÑÐ²Ð»ÐµÐ½Ñ Ð² Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ð¸ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð° GluA2 Ð¸ GluN2A ÑÑÐ±ÑÐµÐ´Ð¸Ð½Ð¸Ñ Ð¸ ÑÐ¾Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ñ GluN2A/GluN2B Ð² Ð´Ð¾ÑÐ·Ð°Ð»ÑÐ½Ð¾Ð¼ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ðµ. Ð Ð¼ÐµÐ´Ð¸Ð°Ð»ÑÐ½Ð¾Ð¹ Ð¿ÑÐµÑÑÐ¾Ð½ÑÐ°Ð»ÑÐ½Ð¾Ð¹ ÐºÐ¾ÑÐµ ÑÑÑÐµÑÑ-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ðµ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ðµ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð° GluA1- ÑÑÐ±ÑÐµÐ´Ð¸Ð½Ð¸ÑÑ Ð¸ Ð½Ðµ Ð·Ð°Ð²Ð¸ÑÐµÐ» Ð¾Ñ Ð²Ð²ÐµÐ´ÐµÐ½Ð¸Ñ ÐÐÐ¡. ÐÑÑÐ²Ð»ÐµÐ½Ð½ÑÐµ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ ÑÐ¾Ð¿ÑÐ¾Ð²Ð¾Ð¶Ð´Ð°Ð»Ð¸ÑÑ Ð½Ð°ÑÑÑÐµÐ½Ð¸ÐµÐ¼ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÑÐºÐ¾Ð³Ð¾ Ð¿Ð¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ Ð¸ Ð¿Ð¾Ð²ÑÑÐµÐ½Ð¸ÐµÐ¼ Ð°Ð¶Ð¸ÑÐ°ÑÐ¸Ð¸ Ð¶Ð¸Ð²Ð¾ÑÐ½ÑÑ Ð¿Ð¾ÑÐ»Ðµ ÑÑÑÐµÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð² ÑÐµÑÑÐµ Â«ÐÑÐºÑÑÑÐ¾Ðµ Ð¿Ð¾Ð»ÐµÂ». ÐÑÐ¾Ð²ÐµÐ´ÑÐ½Ð½Ð¾Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð»Ð¾, ÑÑÐ¾ Ð²Ð²ÐµÐ´ÐµÐ½Ð¸Ðµ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÐÐÐ¡ Ð² ÑÐ°Ð½Ð½ÐµÐ¼ Ð¿Ð¾ÑÑÐ½Ð°ÑÐ°Ð»ÑÐ½Ð¾Ð¼ Ð¾Ð½ÑÐ¾Ð³ÐµÐ½ÐµÐ·Ðµ Ð¿ÑÐ¸Ð²Ð¾Ð´Ð¸Ñ Ðº Ð¾ÑÑÑÐ°Ð²Ð»ÐµÐ½Ð½ÑÐ¼ Ð²Ð¾ Ð²ÑÐµÐ¼ÐµÐ½Ð¸ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸ÑÐ¼ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² ÑÑÐ±ÑÐµÐ´Ð¸Ð½Ð¸Ñ NMDA- Ð¸ AMPA-ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð², Ð¿ÑÐ¸ ÑÑÐ¾Ð¼ Ð¿Ð¾ÑÐ»Ðµ ÑÑÑÐµÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¼ÐµÐ½ÑÐµÑÑÑ ÑÐ°ÑÐ°ÐºÑÐµÑ ÑÑÐ¸Ñ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð¼Ð¾Ð³ÑÑ Ð±ÑÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ Ð´Ð»Ñ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ¸ Ð½Ð¾Ð²ÑÑ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² ÐºÐ¾ÑÑÐµÐºÑÐ¸Ð¸ Ð½ÐµÑÐ²Ð½Ð¾-Ð¿ÑÐ¸ÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð½Ð°ÑÑÑÐµÐ½Ð¸Ð¹, Ð²ÑÐ·Ð²Ð°Ð½Ð½ÑÑ Ð´ÐµÐ¹ÑÑÐ²Ð¸ÐµÐ¼ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÑ Ð°Ð³ÐµÐ½ÑÐ¾Ð² Ð½Ð° ÑÐ°Ð·Ð²Ð¸Ð²Ð°ÑÑÐ¸Ð¹ÑÑ Ð¼Ð¾Ð·Ð³.
description: The study was aimed at the exploration of the alterations in gene expression of NMDA receptor subunits (GluN1, GluN2A, GluN2B) and AMPA receptor subunits (GluA1, GluA2) in the medial prefrontal cortex, the ventral and dorsal areas of the hippocampus, and the amygdala of rats receiving lipopolysaccharide (LPS) administration during the 3rd week of life, under normal conditions and 25 days after vital stress, as well as an analysis of changes in behavior in these animals. The work was performed on male Wistar rats. The level of gene expression was evaluated by real-time RT-PCR, and anxious and exploratory behaviors were assessed in the Open Field and Elevated Plus Maze tests. The LPS treatment during the 3rd week of life caused a decrease in the expression of the genes of the subunits GluA1, GluA2, GluN2A, GluN2B in the dorsal hippocampus and GluA1 and GluA2 in the ventral hippocampus of adult rats. Stress increased the expression level of the GluA2 subunit gene and the GluN2A/GluN2B ratio in the dorsal hippocampus. In the medial prefrontal cortex, the effect of stress was observed in relation to the expression of the GluA1 subunit gene and was not dependent on LPS administration. The revealed changes were accompanied by impaired exploratory behavior and an increased agitation of stressed animals in the Open Field test. The study showed that the administration of bacterial LPS during early postnatal development impacts the expression of the genes of NMDA and AMPA receptor subunits, while the profile of these alterations changes after stress. The results obtained can be used to develop novel methods for the treatment of neuro-psychic disorders caused by the action of bacterial agents on the developing brain.