Additional file 1 of Multiple mycoviruses identified in Pestalotiopsis spp. from Chinese bayberry
Creators: name: Chen, Fangyong
name: Pu, Zhanxu
name: Ni, Haizhi
name: Wang, Yin
name: Yan, Bangguo
Publisher: name: figshare
Year: 2021
Subjects: subject: Space Science
subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Environmental Sciences not elsewhere classified
subject: Ecology
subject: Biological Sciences not elsewhere classified
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Plant Biology
subject: Virology

description: Additional file 1. Figure S1. RT-PCR confirmation of eight viral-like contigs in the mixed RNA sample. See Table S2 for detailed information of primers and amplicon sizes. Figure S2. Pairwise identity comparisons of botoumiaviral (a) and mitoviral (b) sequences in Pestalotiopsis strains to other known botoumiaviruses and mitoviruses. See Table S3 for detailed information of each virus. Figure S3. Conserved amino acid sequence motifs of the putative RNA-dependent RNA polymerases of Colletotrichum gloeosporioides ourmia-like virus 1 (CgOLV1), Pestalotiopsis botourmiavirus 2 (PBV-2), Pestalotiopsis botourmiavirus 3 (PBV-3), Botrytis ourmia-like virus (BOLV), Phoma matteucciicola ourmia-like virus 1 (PmOLV1), Magnaporthe oryzae ourmia-like virus (MOLV), and Rhizoctonia solani ourmia-like virus 1 (RsOLV1). The GenBank accession number of each virus is listed in Table S3. “*” indicates identical amino acid residues; and “.” indicates low chemically similar amino acid residues. Figure S4. Conserved amino acid sequence motifs of the putative RNA-dependent RNA polymerases of Pestalotiopsis mitovirus 1 (PMV-1), Pestalotiopsis mitovirus 2 (PMV-2), Cryphonectria cubensis mitovirus 2a (CcMV2a), Ophiostoma mitovirus 1a (OnuMV1a), Sclerotinia homoeocarpa mitovirus (ShMV), Ophiostoma mitovirus 3a (OnuMV1a), and Cryphonectria parasitica mitovirus 1-NB631 (CpMV1-NB631). The GenBank accession number of each virus is listed in Table S3. “*” indicates identical amino acid residues; and “.” indicates low chemically similar amino acid residues. Figure S5. Multiple alignment of amino acid sequences of conserved domains including methyltransferase (Mtr), viral RNA Helicase (Hel), and RNA-dependent RNA polymerase (RdRp) domains of Pestalotiopsis deltaflexi-like virus 1 (PDFV-1), Sclerotinia sclerotiorum deltaflexivirus 1 (SsDFV1), Sclerotinia sclerotiorum deltaflexivirus 2 (SsDFV2), and Fusarium graminearum deltaflexivirus 1 (FgDFV1). The GenBank accession number of each virus is listed in Table S3. “*” indicates identical amino acid residues; and “.” indicates low chemically similar amino acid residues. Figure S6. Conserved amino acid sequence motifs of the putative RNA-dependent RNA polymerases of Pestalotiopsis negative-stranded RNA virus 1, rose rosette emaravirus (RRV), and fig mosaic emaravirus (FMV). The GenBank accession number of each virus is listed in Table S3. “*” indicates identical amino acid residues; and “.” indicates low chemically similar amino acid residues. Figure S7. Conserved amino acid sequence motifs of the putative RNA-dependent RNA polymerases of Pestalotiopsis partitivirus 1 (PPV-1), beet cryptic virus 1 (BcV1), beet cryptic virus 2 (BcV2), beet cryptic virus 3 (BcV3), and fig cryptic virus (FcV). The GenBank accession number of each virus is listed in Table S3. “*” indicates identical amino acid residues; and “.” indicates low chemically similar amino acid residues. Table S1. Strains used in this study. Table S2. List of PCR primers used for viral contig detection. Table S3. Viruses used for multiple sequence alignments and phylogenetic analysis.