cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Additional file 3: of Development and evaluation of the Andhra Pradesh Children and Parent Study Physical Activity Questionnaire (APCAPS-PAQ): a cross-sectional study
Creators: name: Matsuzaki, Mika
name: Sullivan, Ruth
name: Ekelund, Ulf
name: KV Krishna
name: Kulkarni, Bharati
name: Collier, Tim
name: Ben-Shlomo, Yoav
name: Kinra, Sanjay
name: Kuper, Hannah
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biophysics
subject: Medicine
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Developmental Biology
subject: Science Policy

description: Sensitivity analysis for correlation and Đş agreement reported in the APCAPS-PAQ and parameters derived from the Actiheart monitor for free-living activity, assigning walking a MET value of 2.0. (DOCX 13Â kb)
The High Seas Treaty heralds a new era of global ocean governance
Creators: name: Policy Labs, Frontiers
Publisher: name: Frontiers
Year: 2024
Subjects: Environment Policy

description: After nearly two decades of discussions and a 36-hour marathon of negotiations, Ambassador Rena Lee, President of the United Nations (UN) Intergovernmental Conference, announced on 4 March 2023 that “the ship has reached the shore” for a new, legally binding international treaty to protect marine life in international waters (High Seas Treaty).* This is the first time in over 40 years that world governments have gathered to finalize a treaty related to the ocean, and the first ever to address biodiversity in the high seas—finally putting this shared resource under global protection. In their lead article, Laiolo et al. (1) explain how understanding genomic diversity in the ocean is a critical pathway for science-based decision making to support both ocean conservation efforts and sustainable use of ocean resources. In this UN Decade of Ocean Science, the successful conclusion of the High Seas Treaty negotiations reflects an important shift in the world’s commitment to collectively address some of the most pressing challenges on our blue planet. To successfully implement the Treaty, a comprehensive science-based understanding of marine biodiversity and ecosystems in vast and largely unexplored ocean areas is necessary.
Additional file 6: of Quantitative autism symptom patterns recapitulate differential mechanisms of genetic transmission in single and multiple incidence families
Creators: name: Frazier, Thomas
name: Youngstrom, Eric
name: Hardan, Antonio
name: Georgiades, Stelios
name: Constantino, John
name: Eng, Charis
Publisher: name: Figshare
Year: 2015
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Science Policy
subject: Computational Biology

description: Main effects examining SRS total raw score by family incidence type and family sex type in ASD-affected siblings. This file provides generalized estimating equation results for autism symptom levels by family incidence type and family sex type in ASD-affected children.
MOESM3 of Loss and conservation of evolutionary history in the Mediterranean Basin
Creators: name: S. Veron
name: P. Clergeau
name: S. Pavoine
Publisher: name: Figshare
Year: 2016
Subjects: subject: Cell Biology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Ecology
subject: FOS: Biological sciences
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics
subject: Inorganic Chemistry
subject: FOS: Chemical sciences
subject: Hematology
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Additional file 3. Identification of priority sites independently of Aïchi targets in amphibians. Data: A. Identification of Expected PDloss priority sites independently of Aïchi targets. Expected PDloss hotspots were defined as areas where Expected PDloss was higher than the mean value of all sites and areas where Expected PDloss was higher than under a random distribution of threats (FExpected PDloss ≥ 0.5). B and C. Identification of HEDGE and BED priority sites independently of Aïchi targets: HEDGE and BED hotspots were defined as areas where HEDGE and BED, respectively, contained at least one species from the 10 % of species with the highest HEDGE and BED scores and areas where HEDGE and BED, respectively, was higher than under a random distribution of threats (FHEDGE/BED ≥ 0.5).
Halictus scabiosae
Creators: name: Zettel, Herbert
name: Ockermüller, Esther
name: Schoder, Sabine
name: Seyfert, Franz
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hymenoptera
Halictidae
Halictus
Halictus scabiosae

description: Halictus scabiosae (ROSSI, 1790), Gelbbindige Furchenbiene
N a c h w e i s e: 10. Bez., Umgebung U1-Station Altes Landgut, N 48°09,7′, E 16°23,0′, 240 m SH, F&B-T13, 26.VII.2020, 2♀♀, ldcHZ; 13. Bez., Lainz, Krankenhausareal, N 48°10,47′, E 16°16,27′, 245 m SH, F&B-R06, 21.VI.2019, 1♁, lFS, dcHZ; 22. Bez., Obere Lobau, Halbtrockenrasen, N 48°11,9′, E 16°31,3 –31,7′, 155 m SH, F&B-P21, 12.VIII.2019, 1♀, ldcHZ; 22. Bez., Obere Lobau, E Tischwasser, N 48°11,6′, E 16°29,1′, 154 m SH, F&B-P19, 18.VIII.2019, 1♀, ldcHZ; 22. Bez., Seestadt, Umgebung U2-Station, N 48°13,55′, E 16°30,5′, 157 m SH, F&B-L20, 10.VII.2020, 1♀, ldcHZ; 22. Bez., Donauinsel bei Schleusenbrücke, N 48°12,7′, E 16°26,05′, 165 m SH, F&B-N16, 11.VI.2021, 1♀, ldcHZ.
description: Published as part of Zettel, Herbert, Ockermüller, Esther, Schoder, Sabine & Seyfert, Franz, 2022, Zur Verbreitung der Wildbien en (Hymenoptera, Apidae) in Wien, Österreich, pp. 351-396 in Linzer biologische Beiträge 54 (1) on page 368, DOI: 10.5281/zenodo.7507600
Halictus submediterraneus
Creators: name: Zettel, Herbert
name: Ockermüller, Esther
name: Schoder, Sabine
name: Seyfert, Franz
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hymenoptera
Halictidae
Halictus
Halictus submediterraneus

description: Halictus submediterraneus (PAULY, 2015), Südliche Goldfurchenbiene
N a c h w e i s e: 2. Bez., Nordbahnhofgelände, N 48°13,7 –13,8′, E 16°23,35 –23,55′, 165 m SH, F&B-L13, 27.VII.2016, 1♀, ldcHZ; 3.VIII.2016, 1♀, ldcHZ; 21. Bez., Stammersdorf, Brache, N 48°18,87′, E 16°25,08′, 222 m SH, F&B-B15, 5.VIII.2007, 2♀♀, ldcHZ.
description: Published as part of Zettel, Herbert, Ockermüller, Esther, Schoder, Sabine & Seyfert, Franz, 2022, Zur Verbreitung der Wildbien en (Hymenoptera, Apidae) in Wien, Österreich, pp. 351-396 in Linzer biologische Beiträge 54 (1) on page 368, DOI: 10.5281/zenodo.7507600
Additional file 1: Figure S1. of Vitamin A deficiency modulates iron metabolism independent of hemojuvelin (Hfe2) and bone morphogenetic protein 6 (Bmp6) transcript levels
Creators: name: Mendes, Juliana
name: Siqueira, Egle
name: Silva, João De Brito E
name: Arruda, Sandra
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biochemistry
subject: Medicine
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Developmental Biology
subject: Inorganic Chemistry

description: Iron concentration in the liver (A), small intestine (B), and spleen (C) of rats treated with diets containing different sources and amounts of vitamin A and iron for 59 days. Mean ± standard deviation, * p
MEMS-based anti-biofouling - mechanism, devices and application
Creators: name: Yeh, Po Ying
Publisher: name: University of British Columbia
Year: 2009

description: A novel anti-biofouling mechanism based on the combined effects of electric field and shear stress was reported. The mechanism was observed in millimeter-scale piezoelectric plates coated with different metal materials and microfabricated Micro-Electro-Mechanical Systems (MEMS) devices. Experimental observation on the quantities of protein desorption and theoretical calculations on surface interactions (van der Waals, electrostatic, hydrophobic, shear stress) have been carried out. This anti-fouling mechanism can also be activated by a vibrating micromachined Si/SiO₂ membrane. The combined effect of polyethylene glycol (PEG) grafting and application of vibration on attenuation of protein adsorption was also investigated. Vibrating PEG-grafted surfaces significantly attenuate protein adsorption, especially at low PEG grafting densities. Polymer steric interaction dominates over vibration interaction with protein on surfaces with high PEG grafting densities. Monothiol-functionalized hyperbranched polyglycidols (HPG-SH) were synthesized and self-assembled on the gold surface. The characteristics of the polymer were studied and compared with linear PEG using various surface analysis techniques. This hyperbranched polyglycidol is more resistant to protein adsorption than is linear PEG of similar molecular weight. In addition, higher molecular weight HPG shows less protein adsorption than does lower molecular weight HPG. The hyperbranched polyglycidols (without a thiol group) were further modified to generate functionality for microchannel-based liquid chromatography applications. The microchannel surface was first amino modified by allylamine plasma, and amino groups then reacted with N-hydroxy succinimide-functionalized HPGs to form strong amide bonds. The grafted HPGs are resistant to nonspecific protein adsorption. The succinimidyl ester groups degrade in water to form carboxyl groups on HPGs. By giving extra carboxyl groups to each HPG, the HPG can selectively capture positive avidin from a mixture of avidin and bovine serum albumin (BSA). To increase the capture efficiency, the microchannel was integrated with micropillar arrays as the liquid chromatography column.
Heriades rubicola PEREZ 1890
Creators: name: Zettel, Herbert
name: Ockermüller, Esther
name: Schoder, Sabine
name: Seyfert, Franz
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hymenoptera
Megachilidae
Heriades
Heriades rubicola

description: Heriades rubicola PÉREZ, 1890, Stängel-Löcherbiene
N a c h w e i s e: 2. Bez., Nordbahnhofgelände, N 48°13,7 –13,8′, E 16°23,35 –23,55′, 165 m SH, F&B-L13, 25.VIII.2016, 1♀, lFS, dcHZ; N 48°13,85′, E 16°23,4′, 165 m SH, F&B-L13, 8.VII.2017, 1♁, ldcHZ; 22. Bez., Blumengärten Hirschstetten, N 48°14′, E 16°29′, 159 m SH, F&B-K18, 28. VI.2016, 1♁, lFS, dcHZ; 22. Bez., Blumengärten Hirschstetten, Beetmischung "Wiener Melange", N 48°14,34′, E 16°28,52′, 159 m SH, F&B-K18, 7.VIII.2019, 1♁, leg. Daniela Lehner, dHZ, cBFA.
description: Published as part of Zettel, Herbert, Ockermüller, Esther, Schoder, Sabine & Seyfert, Franz, 2022, Zur Verbreitung der Wildbien en (Hymenoptera, Apidae) in Wien, Österreich, pp. 351-396 in Linzer biologische Beiträge 54 (1) on page 368, DOI: 10.5281/zenodo.7507600
MOESM6 of Loss and conservation of evolutionary history in the Mediterranean Basin
Creators: name: S. Veron
name: P. Clergeau
name: S. Pavoine
Publisher: name: Figshare
Year: 2016
Subjects: subject: Evolutionary Biology
subject: FOS: Biological sciences
subject: Ecology
subject: Developmental Biology
subject: Inorganic Chemistry
subject: FOS: Chemical sciences
subject: Computational Biology

description: Additional file 6. Spatial distribution of species richness in amphibians. A. Species richness. B. Threatened species richness (number of species whose threat status is CR, EN or VU). C. Endemic species richness (number of species among the 10Â % of species with the smallest range).
Additional file 2: Fig. S1. of Genome-wide miR-155 and miR-802 target gene identification in the hippocampus of Ts65Dn Down syndrome mouse model by miRNA sponges
Creators: name: Ros, Xavier Bofill-De
name: Santos, Mónica
name: Vila-Casadesús, Maria
name: Villanueva, Eneko
name: Andreu, Nuria
name: Dierssen, Mara
name: Fillat, Cristina
Publisher: name: Figshare
Year: 2015
Subjects: subject: Biochemistry
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Physiology
subject: 69999 Biological Sciences not elsewhere classified
subject: 111714 Mental Health
subject: FOS: Health sciences
subject: 60506 Virology

description: a) Schematic representation of lentiviral vectors used to overexpress mmu-miR-155 and mmu-miR-802 (p-miR155 and p-miR802). b) Expression of mmu-miR155 and miR-802 in HeLa cells transfected with p-miR155 and p-miR802 (HeLa-miR-155-miR802). c) d2EGFP expression in HeLa and HeLa-miR-155-miR802 transduced with Lv-Control Lv-miR155T or Lv-miR155-802T. Top panel shows representative images of d2EGFP positive cells at 72-hours after transduction with 10 MOI of Lv-Control, LvmiR155Tor Lv-miR155-802T. Bottom panel shows the number of d2EGFP positive cells at different viraldoses. Image analysis was assessed using ImageJ software. Fig. S2. a) Principal component analysis of data obtained with the Agilent SurePrint G3 Mouse gene expression 8x60K Microarray (ID 028005). TheGEO accession number for our microarray data reported in this paper is GSE68074. Table of meandistances among the different genotypes and treatments. b) Prediction of miRNA targets based oncorrelation coefficients obtained from hippocampus treatment with sponge lentiviruses by different methods.Left panel shows the correlation prediction compared to the number of target sites predicted by TargetScan.Central panel shows the correlation prediction compared to the probability of conserved targeting(TargetScan PCT score). Right panel shows the correlation prediction compared to TargetScan contextscore. Fig. S3. a) Representation of Down’s syndrome gene expression dysregulation domanins (GEDDs)described by Letourneau and collaborators (black barplot overlay), and changes in expression in the miceorthologous genes observed in hippocampus upon the treatment with Lv-miR155-802T (grey barplotoverlay). Representation was performed using CIRCOS visualization package. b) Venn diagram of miRNApredicted targets on Lv-miR155-802T treated hippocampus based on the negative coefficients obtainedfrom miRComb analysis package for miR-155 (Corr_155 -) and miR-802 (Corr_802 -), genes that presenteda negative fold-change in between euploid and trisomic condition (TS/WT -) and genes that presented apositive fold-change in tisomic cells upon the XIST-induced HSA21 silencing (TS-XIST/TS +). (DOCX 3411 kb)
Additional file 1: of Quantitative autism symptom patterns recapitulate differential mechanisms of genetic transmission in single and multiple incidence families
Creators: name: Frazier, Thomas
name: Youngstrom, Eric
name: Hardan, Antonio
name: Georgiades, Stelios
name: Constantino, John
name: Eng, Charis
Publisher: name: Figshare
Year: 2015
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Inorganic Chemistry
subject: FOS: Chemical sciences
subject: Science Policy
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Social Communication Questionnaire (SCQ) and Social Responsiveness Scale (SRS) item mapping to DSM-5 criteria. This file provides the SCQ and SRS items which map to each of the DSM-5 criteria to create DSM symptom scores.
DEMUCS for data-driven RF signal denoising
Creators: Cagkan Yapar
Fabian Jaensch
Jan C. Hauffen
Francesco Pezone
Peter Jung
Saeid K. Dehkordi
Giuseppe Caire
Publisher: name: IEEE Signal Processing Society
Year: 2024

description: In this paper, we present our radio frequency signal denoising approach, RFDEMUCS, for the 2024 IEEE ICASSP RF Signal Separation Challenge. Our approach is based on the DEMUCS architecture [1], and has a U-Net structure with a bidirectional LSTM bottleneck. For the task of estimating the underlying bit-sequence message, we also propose an extension of the DEMUCS that directly estimates the bits. Evaluations of the presented methods on the challenge test dataset yield MSE and BER scores of −118.71 and −81, respectively, according to the evaluation metrics defined in the challenge.
Hoplitis claviventris
Creators: name: Zettel, Herbert
name: Ockermüller, Esther
name: Schoder, Sabine
name: Seyfert, Franz
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hymenoptera
Megachilidae
Hoplitis
Hoplitis claviventris

description: Hoplitis claviventris (THOMSON, 1872), Gelbspornige Stängelbiene
N a c h w e i s: 22. Bez., Untere Lobau, N 48°09,5′, E 16°32,5′, 152 m SH, F&B-T22, 22.VI.2013, 1♁, lEO, dHZ, cEO.
description: Published as part of Zettel, Herbert, Ockermüller, Esther, Schoder, Sabine & Seyfert, Franz, 2022, Zur Verbreitung der Wildbien en (Hymenoptera, Apidae) in Wien, Österreich, pp. 351-396 in Linzer biologische Beiträge 54 (1) on page 369, DOI: 10.5281/zenodo.7507600
Additional file 2: Table S1. of Vitamin A deficiency modulates iron metabolism independent of hemojuvelin (Hfe2) and bone morphogenetic protein 6 (Bmp6) transcript levels
Creators: name: Mendes, Juliana
name: Siqueira, Egle
name: Silva, João De Brito E
name: Arruda, Sandra
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biochemistry
subject: Medicine
subject: Biotechnology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics
subject: Developmental Biology
subject: Inorganic Chemistry
subject: FOS: Chemical sciences
subject: Hematology

description: Hematological parameters of rats treated with diets containing different sources and amounts of vitamin A and iron, for 59 days. (DOCX 30.5 kb)
Halictus tectus RADOSZKOWSKI 1985
Creators: name: Zettel, Herbert
name: Ockermüller, Esther
name: Schoder, Sabine
name: Seyfert, Franz
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hymenoptera
Halictidae
Halictus
Halictus tectus

description: Halictus tectus RADOSZKOWSKI, 1985, Kleine Filzfurchenbiene
N a c h w e i s: 21. Bez., Stammersdorf, Alte Schanze XI, N 48°19,25′, E 16°25,3′, 219 m SH, F&B-A15, 24.VI.2020, 1♀, ldcHZ.
A n m e r k u n g: Neben historischen Funden (vgl. ZETTEL et al. 2015), gibt es ausser dem oben genannten noch einen weiteren Wiederfund in Wien (siehe PACHINGER et al. 2020).
description: Published as part of Zettel, Herbert, Ockermüller, Esther, Schoder, Sabine & Seyfert, Franz, 2022, Zur Verbreitung der Wildbien en (Hymenoptera, Apidae) in Wien, Österreich, pp. 351-396 in Linzer biologische Beiträge 54 (1) on page 368, DOI: 10.5281/zenodo.7507600
Galling adelgids : gall formation, developmental morphology, characterization, and the genetic susceptibility of spruce
Creators: name: Bains, Babita
Publisher: name: University of British Columbia
Year: 2009

description: Adelgids (Hemiptera: Adelgidae) are a small group of insects with complex life cycles and can threaten seed production in British Columbia (BC). Adelgids induce galls on the reproductive and vegetative shoots of spruce trees, reducing the number of future cone sites. Additionally, feeding on the cones and needles of alternate conifer hosts can reduce seed extraction efficiency, and cause discoloration and twisting of needles. Seed orchards are intensively managed for frequent and abundant seed production; hence losses incurred by adelgid pests are of high importance. Considering the complexity of insect-galler systems, there are many unanswered questions regarding the basic biology and habits of adelgids. My research elucidates the influence of two adelgid life stages, the fundatrix and her offspring the gallicolae, through the use of manipulative laboratory experiments and botanical histological processes. It is evident that fundatrices induce the gall formation process and gallicolae activity completes gall formation. Considering the fundatrix is required to stimulate galling it would be sufficient to include only fundatrices in a monitoring system. Additionally, I tested the theory that susceptibility to adelgid induced galling is under genetic control in spruce. Strong evidence of genetic control of susceptibility was observed with modest individual and high half- and full-sib family narrow-sense heritabilities of 0.17±0.09, 0.87±0.04, and 0.61±0.16, respectively. Breeding values for parental susceptibility to adelgid attack ranged between 0.25 and 0.76, indicating that selection for reduced susceptibility would be possible. These results suggest that adelgid galling susceptibility could be used as a screening criterion for parental inclusion in future seed orchard establishment. Furthermore, I attempted to associate structurally unique galls with the inducing adelgid species. Detailed associations of adelgid species and their galls have not been rigorously determined. Rearing of gallicolae alates from various galls suggests that there can be a wide range of variation in the structure that a single species induces. Characterization of galls may allow orchard managers to understand what adelgid species are present and possibly avoid the taxonomic expertise required to identify adelgids.
Additional file 3: figure S3. of Biochemical and biophysical characterization of cell-free synthesized Rift Valley fever virus nucleoprotein capsids enables in vitro screening to identify novel antivirals
Creators: name: Broce, Sean
name: Hensley, Lisa
name: Tomoharu Sato
name: Lehrer-Graiwer, Joshua
name: Essrich, Christian
name: Edwards, Katie
name: Pajda, Jacqueline
name: Davis, Christopher
name: Bhadresh, Rami
name: Hurt, Clarence
name: Freeman, Beverly
name: Vishwanath Lingappa
name: Kelleher, Colm
name: Karpuj, Marcela
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biochemistry
subject: Medicine
subject: Molecular Biology
subject: Pharmacology
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: Plant Biology
subject: 60506 Virology
subject: Computational Biology

description: Transcript concentration in the in vitro translation reaction affects RVFV NP distribution on the sucrose gradients. Note that the amplitude of peak II (see Additional file 3: figure S3), representing high-order assembly structures, required higher amounts of RNA. The reactions were carried out at 26Â Â°C, conditions that favor the accumulation of intermediates; accordingly, a substantial amount of the material was always found in peak I, representing the assembly intermediates. See also Additional file 4: figure S4 for optimization of the conditions that enable a clear distinction between the peaks representing the partially and highly ordered assembled structures. (PDF 57 kb)
Nilssonia undetermined
Creators: name: Flores-Barragan, Miguel Angel
name: Leon, María Patricia Velasco-de
Publisher: name: Zenodo
Year: 2021
Subjects: Biodiversity
Taxonomy
Animalia
Chordata
Testudines
Cryptodira
Trionychidae
Nilssonia
Nilssonia undetermined

description: Nilssonia sp.
Figure 4 A-B
Material. CFZ-MTZ266.
Locality. Carretera.
Description. Leaf pinnate (segmented) is subdivided into numerous, regular oppositely positioned segments. The leaf is 6.3 cm long and 4.7 cm wide in the middle part. It has segments with a constant width from 1 to 1.2 cm wide at the base, and the leaf apex is unknown. The distance between the segments is relatively constant at about 0.2-0.3 cm. The lamina is attached to the upper edge of rachis and is completely covered by the rachis, which can measure up to 0.3 cm in width. The venation of the segments is inserted at an angle of 80-90°. The veins can dichotomize near the base then become parallel with a density of 12 to 17 veins per cm in the proximal part to the margin.
Remarks. Segmented leaves have been reported for the Permian from different parts of the world, such as Pterophyllum cotteanaum Gutbier from the lower Permian of Germany (Barthel et al., 2010), Pseudoctenis middridgensis Stonley from the upper Permian of England (Stonley, 1958), and/or Pseudoctenis samchokense, Kawasaki from the upper Permian of China (Pott et al., 2010). However, in these cases, the lamina segments are laterally attached to the rachis and are not similar to the Puebla specimen (Table 3). The leaf described in the present work is assigned to Nilssonia based on the leaf lamina clearly being attached to the upper edge of the rachis (Van Konijnenburg-van Cittert, et al., 2017). Nilssonia has some records for the upper Paleozoic (Tian and Zhang 1980; He et al., 1996), and the first reliable reports are from the Triassic. The species Nilssonia sturii Krasser from the upper Triassic of Austria (Pott et al., 2007) evinces some similarity to the specimen from Mexico but differs from it by having a lower number of veins, as well as the presence of dichotomies in the Mexican specimen.
It is important to mention that the dichotomies in the veins in the genus Nilssonia are not common in species from the Northern Hemisphere, but are common in species from Gondwana (Holmes et al., 2010). Therefore, it is not comparable to other species described for the Triassic of Europe (Barbacka, 2001; Kustatscher and Van Konijnenburg-van Cittert, 2010).
description: Published as part of Flores-Barragan, Miguel Angel & Leon, María Patricia Velasco-de, 2020, New records of Bjuvia and Nilssonia from the Permian of Mexico, pp. 1-17 in Palaeontologia Electronica (a 28) (a 28) 24 (3) on pages 8-10, DOI: 10.26879/1109, http://zenodo.org/record/10961885
Hoplitis papaveris
Creators: name: Zettel, Herbert
name: Ockermüller, Esther
name: Schoder, Sabine
name: Seyfert, Franz
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hymenoptera
Megachilidae
Hoplitis
Hoplitis papaveris

description: Hoplitis papaveris (LATREILLE, 1799), Mohnbiene
N a c h w e i s: 22. Bez., Essling, Bio Forschung Austria, N 48°12,7′, E 16°31,25′, 155 m SH, F& B-N21, 21.V.2021, 1♁, leg. K. Zenz, dHZ, cBFA.
A n m e r k u n g: Die vereinzelten Funde von Männchen (PACHINGER et al. 2020, dieser Nachweis) dürfen nicht darüber hinwegtäuschen, wie selten und gefährdet die Mohnbiene in der gesamten Region ist. Am früheren Niststandort der Alten Schanze XI (siehe ZETTEL et al. 2016) konnten seit Jahren keine Nester mehr festgestellt werden.
description: Published as part of Zettel, Herbert, Ockermüller, Esther, Schoder, Sabine & Seyfert, Franz, 2022, Zur Verbreitung der Wildbien en (Hymenoptera, Apidae) in Wien, Österreich, pp. 351-396 in Linzer biologische Beiträge 54 (1) on page 369, DOI: 10.5281/zenodo.7507600
[Thank You Message from Kamloops Chinese Cultural Association to the Dang Family]
[甘露市中華文化協會致 Dang 氏家族的感謝詞]
Creators: name: Kamloops Chinese Cultural Association
Publisher: name: The University of British Columbia
Year: 2012

description: Message from the Kamloops Chinese Cultural Association thanking the Dang family for donating material to be presented as a part of Chinese Canadian history.
Taeniopteris lentriculiformis Walkom 1917
Creators: name: Flores-Barragan, Miguel Angel
name: Leon, María Patricia Velasco-de
Publisher: name: Zenodo
Year: 2021
Subjects: Biodiversity
Taxonomy
Plantae
Tracheophyta
Polypodiopsida
Polypodiales
Pteridaceae
Taeniopteris
Taeniopteris lentriculiformis

description: Taeniopteris lentriculiformis (Etheridge) Walkom, 1917
Figure 4 C-D
Material. CFZMTZ-330.
Locality. Coatepec.
Description. Entire lamina, with entire margins, probably oblong in shape, leaf width appears to be constant. The apex and base are not preserved. The leaf is 4.1 cm long and 2.5 cm wide. The lamina attaches laterally to the rachis. The rachis is slim, retaining its width throughout the whole leaf with a maximum width of 0.2 cm. The fine veins emanate from the rachis at 90°. The venation is simple, rarely dichotomous, with a density of 23 to 24 veins per cm at the middle-part of the leaf.
Remarks. Given the general morphology of the leaf, this specimen resembles an isolated pinna of the genus Danaeopsis Heer ex Schimper. However, this taxon is characterized by having strong secondary veins that anastomose near the margin. Even though there are species that can lack anastomoses, such as D. angustifolia and D. fecunda (Kustatscher et al., 2012a), the differences in leaf size, vein angle, and venation density do not allow assignation to these species. In addition, the lack of compound leaves and/or sporangia that determine the affinity of these specimens makes their comparison with this genus impossible. For this analysis, the specimen from Puebla was compared with 42 species of the fossil taxon Taeniopteris described for the Permian-Triassic (Table 4). The observations indicate a higher similarity with the species Taeniopteris lentriculiformis, which is characterized by the near absence of bifurcations in the veins. Yet the size of its lamina is comparable to that of T. vittata Brongniart, it does differ due to the lower venation density of T. lentriculiformis (Artabe, 1985). This species has been reported for the Triassic of Argentina, New Zealand, and Australia (Artabe, 1985; Rettallack, 1985).
description: Published as part of Flores-Barragan, Miguel Angel & Leon, María Patricia Velasco-de, 2020, New records of Bjuvia and Nilssonia from the Permian of Mexico, pp. 1-17 in Palaeontologia Electronica (a 28) (a 28) 24 (3) on page 11, DOI: 10.26879/1109, http://zenodo.org/record/10961885
MOESM2 of Loss and conservation of evolutionary history in the Mediterranean Basin
Creators: name: S. Veron
name: P. Clergeau
name: S. Pavoine
Publisher: name: Figshare
Year: 2016
Subjects: subject: Cell Biology
subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Ecology
subject: FOS: Biological sciences
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics
subject: Inorganic Chemistry
subject: FOS: Chemical sciences
subject: Hematology
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Additional file 2. Identification of priority sites independently of Aïchi targets in squamates. Data. A. Identification of Expected PDloss priority sites independently of Aïchi targets. Expected PDloss hotspots were defined as areas where Expected PDloss was higher than the mean value of all sites and areas where Expected PDloss was higher than under a random distribution of threats (FExpected PDloss ≥ 0.5). B and C. Identification of HEDGE and BED priority sites independently of Aïchi targets: HEDGE and BED hotspots were defined as areas where HEDGE and BED, respectively, contained at least one species from the 10 % of species with the highest HEDGE and BED scores and areas where HEDGE and BED, respectively, was higher than under a random distribution of threats (FHEDGE/BED ≥ 0.5).
Additional file 7: figure S7. of Biochemical and biophysical characterization of cell-free synthesized Rift Valley fever virus nucleoprotein capsids enables in vitro screening to identify novel antivirals
Creators: name: Broce, Sean
name: Hensley, Lisa
name: Tomoharu Sato
name: Lehrer-Graiwer, Joshua
name: Essrich, Christian
name: Edwards, Katie
name: Pajda, Jacqueline
name: Davis, Christopher
name: Bhadresh, Rami
name: Hurt, Clarence
name: Freeman, Beverly
name: Vishwanath Lingappa
name: Kelleher, Colm
name: Karpuj, Marcela
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biochemistry
subject: Cell Biology
subject: Pharmacology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: 60506 Virology
subject: Computational Biology

description: Conversion of the IOAS into IHOS by nonradioactive CFPS-products is not affected by Apyrase. Glycerol-gradient fractionation profiles of the radioactive material generated in CFPs under conditions favoring intermediate-assembly structures and “chased” by the addition of nonradioactive products for 120 min and carried out in the presence or absence of the ATP inhibitor Apyrase (final concentration of 0.5 U/μl in the cell-free reaction mixture) at 37 °C. Note that in all cases, in addition to the chase at 26 °°C, the material in peak I (intermediate assembly structures) was converted to the highly ordered assembly structures (Peak II), indicating that the process of RVFV NP assembly is indeed independent of energy but is temperature dependent. (PDF 88 kb)
Additional file 9: of Sex chromosome evolution in snakes inferred from divergence patterns of two gametologous genes and chromosome distribution of sex chromosome-linked repetitive sequences
Creators: name: Matsubara, Kazumi
name: Nishida, Chizuko
name: Matsuda, Yoichi
name: Kumazawa, Yoshinori
Publisher: name: Figshare
Year: 2016
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Evolutionary Biology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: Plant Biology

description: Molecular phylogenic trees of CTNNB1 gene. This figure shows neighbor-joining trees of CTNNB1 gene with the long alignment for 20 tetrapod species and the short alignment for 26 squamate species. (PDF 289 kb)
Hylaeus difformis
Creators: name: Zettel, Herbert
name: Ockermüller, Esther
name: Schoder, Sabine
name: Seyfert, Franz
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hymenoptera
Colletidae
Hylaeus
Hylaeus difformis

description: Hylaeus difformis (EVERSMANN, 1852), Beulen-Maskenbiene
N a c h w e i s e: 3. Bez., Botanischer Garten, N 48°11,57′, E 16°23,05′, 185 m SH, F&B-P13, 20.VI.2017, 1♁, ldcSS; 13. Bez., Lainzer Tiergarten, S Wiener Blick, N 48°10,75′, E 16°14,8′, 400 m SH, F&B-R04, 17.VII.2013, 1♀, ldcHZ; 13. Bez., Lainzer Tiergarten, Johannser Kogel und Wiese, N 48°11,1′, E 16°13,3′, 300 m SH, F&B-Q03, 15.VI.2017, 2♀♀, lFS & lHZ, dcHZ; 16. Bez., Kreuzeichenwiese, N 48°13,55′, E 16°16,3′, 440 m SH, F&B-M06, 11.VI.2022, 1♁, ldcHZ.
description: Published as part of Zettel, Herbert, Ockermüller, Esther, Schoder, Sabine & Seyfert, Franz, 2022, Zur Verbreitung der Wildbien en (Hymenoptera, Apidae) in Wien, Österreich, pp. 351-396 in Linzer biologische Beiträge 54 (1) on page 370, DOI: 10.5281/zenodo.7507600
Additional file 7: of Sex chromosome evolution in snakes inferred from divergence patterns of two gametologous genes and chromosome distribution of sex chromosome-linked repetitive sequences
Creators: name: Matsubara, Kazumi
name: Nishida, Chizuko
name: Matsuda, Yoichi
name: Kumazawa, Yoshinori
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biochemistry
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Evolutionary Biology
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: Plant Biology

description: Alignment of full-length amino acid sequences of CTNNB1 among tetrapod species. This figure shows alignment of full-length amino acid sequences of CTNNB1 among 11 tetrapod species. (PDF 306 kb)
Taeniopteris crassinervis Arber 1917
Creators: name: Flores-Barragan, Miguel Angel
name: Leon, María Patricia Velasco-de
Publisher: name: Zenodo
Year: 2021
Subjects: Biodiversity
Taxonomy
Plantae
Tracheophyta
Polypodiopsida
Polypodiales
Pteridaceae
Taeniopteris
Taeniopteris crassinervis

description: Taeniopteris crassinervis (Feistmantel) Arber, 1917
Figure 4 E-F
Material. CFZMTZ-713.
Locality. Coatepec.
Description. Entire lamina, with entire margins, 6.8 cm long and 5.3 cm wide at the mid-part. The leaf width appears to be constant. The shape of the base and apex are unknown. Undulations are observed in the lamina, which could be the result of desiccation and/or the fossilization process. The lamina attaches laterally to the rachis. The rachis is slim, retaining its width throughout the whole leaf with a maximum width of 0.3 cm. The veins emanate from the rachis at 90°. This venation divides at the proximal part of the rachis and then becomes parallel along it. It can have a density of up to 14 to 16 veins per centemeter at the mid-part of the leaf. Remarks. After comparing it with 42 different species of the genus (Table 4), the specimen from Puebla was assigned to the species Taeniopteris crassinervis, since it exhibits strong veins with a similar venation density and perpendicular angle at which they are inserted regarding to the rachis. Another diagnostic characteristic of this species is the dichotomy of the veins in the contact area with the rachis (Figure 4E). This species has been reported for the Triassic of Argentina (Artabe, 1985), United States, India, Australia, New Zealand, and Africa (Beherensmeyer and Turner, 2019). This new report would correspond to the most southern record of North America.
description: Published as part of Flores-Barragan, Miguel Angel & Leon, María Patricia Velasco-de, 2020, New records of Bjuvia and Nilssonia from the Permian of Mexico, pp. 1-17 in Palaeontologia Electronica (a 28) (a 28) 24 (3) on page 11, DOI: 10.26879/1109, http://zenodo.org/record/10961885
Lyricibracon Quicke 1988
Creators: name: Ranjith, A. P.
name: Santhosh, S.
name: Nasser, M.
Publisher: name: Zenodo
Year: 2017
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hymenoptera
Braconidae
Lyricibracon

description: Lyricibracon Quicke, 1988
Type species. Lyricibracon bicolorus Quicke (monotypic). Quicke, 1988: 413.
Diagnosis. Terminal flagellomere acuminate. Median flagellomeres longer than wide. Scapus rather narrow, shorter ventrally than dorsally in lateral aspect, apically obtuse in dorsal aspect, depressed medially in lateral view. Hypoclypeal depression bordered by a transverse carina. Clypeus distinctly separated from face by a transverse groove. Face transversely corrugated or rugose with transverse striations posteriorly. Eyes glabrous. Frons with medial longitudinal groove. Vertex smooth or rugose. Mesoscutum smooth or transversely striate, setose. Notauli distinct, complete. Scutellar sulcus widely crenulated. Scutellum densely setose. Mesopleuron coarsely sculptured. Median area of metanotum with a complete mid-longitudinal carina, with a spine in lateral view. Propodeum with a complete medial longitudinal carina. Fore wing vein 1-SR + M very weakly curving posteriorly after arising from 1-M. Vein cu-a interstitial or slightly postfurcal. Claws with pointed basal lobe. First metasomal tergite with complete, lamelliform dorso-lateral carinae. Tergites 2–6 largely with coarse rugose sculpture. Second metasomal tergite without a mid-basal area, with a number of strong, longitudinal striae anteriorly or coarsely rugose. Second metasomal suture crenulate. Metasomal tergites 3–5 with a transverse, crenulate, subposterior groove. Posterior margin of sixth metasomal tergite straight or slightly emarginate medially, but with a pair of semicircular lateral emarginations resulting in pointed or blunt postero-lateral corners. Ovipositor without pre-apical dorsal nodus and ventral serrations.
Hosts. Unknown.
Distribution. Oriental (Brunei and India).
description: Published as part of Ranjith, A. P., Santhosh, S. & Nasser, M., 2017, Range extension of the rare braconine genus, Lyricibracon Quicke (Hymenoptera: Braconidae), with the description of a new species from India, pp. 422-430 in Zootaxa 4227 (3) on page 423, DOI: 10.11646/zootaxa.4227.3.8, http://zenodo.org/record/268376
Additional file 9: figure S9. of Biochemical and biophysical characterization of cell-free synthesized Rift Valley fever virus nucleoprotein capsids enables in vitro screening to identify novel antivirals
Creators: name: Broce, Sean
name: Hensley, Lisa
name: Tomoharu Sato
name: Lehrer-Graiwer, Joshua
name: Essrich, Christian
name: Edwards, Katie
name: Pajda, Jacqueline
name: Davis, Christopher
name: Bhadresh, Rami
name: Hurt, Clarence
name: Freeman, Beverly
name: Vishwanath Lingappa
name: Kelleher, Colm
name: Karpuj, Marcela
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biochemistry
subject: Cell Biology
subject: Pharmacology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: Developmental Biology
subject: Hematology
subject: 60506 Virology

description: Cell fractionation indicates that the compounds access the nucleus. The most potent compounds were exposed to HEK cells at a concentration of 1Â ÎźM for 24Â h, after which the nucleus was separated from the cytoplasm. The concentration of these two blue compounds could be observed by the relative higher intensity in the nucleus compared to that in the cytoplasm. (PDF 3721 kb)
Hoplitis tridentata
Creators: name: Zettel, Herbert
name: Ockermüller, Esther
name: Schoder, Sabine
name: Seyfert, Franz
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hymenoptera
Megachilidae
Hoplitis
Hoplitis tridentata

description: Hoplitis tridentata (DUFOUR & PERRIS, 1840), Dreizahn-Stängelbiene
N a c h w e i s e: 2. Bez., Nordbahnhofgelände, N 48°13,7 –13,8′, E 16°23,35 –23,55′, 165 m SH, F&B-L13, 3.VI.2017, 1♁, ldcHZ; 21. Bez., Stammersdorf, Wiese mit Birken, N 48°19,14′, E 16°23,6′, 277 m SH, F&B-A13, 1.VII.2007, 3♀♀, ldcHZ; 22. Bez., Breitenlee, Schafflerhof, Sandgrube, N 48°14,4′, E 16°32,3′, 155 m SH, F&B-J22, 14.VI.2001, 1♁, ldcHZ.
description: Published as part of Zettel, Herbert, Ockermüller, Esther, Schoder, Sabine & Seyfert, Franz, 2022, Zur Verbreitung der Wildbien en (Hymenoptera, Apidae) in Wien, Österreich, pp. 351-396 in Linzer biologische Beiträge 54 (1) on page 369, DOI: 10.5281/zenodo.7507600
Additional file 1: of The safety climate in primary care (SAP-C) study: study protocol for a randomised controlled feasibility study
Creators: name: SinĂŠad Lydon
name: Cupples, Margaret
name: Hart, Nigel
name: Murphy, Andrew
name: Faherty, Aileen
name: OâConnor, Paul
Publisher: name: Figshare
Year: 2016
Subjects: subject: Space Science
subject: Medicine
subject: Sociology
subject: FOS: Sociology

description: Invitation letters, participant information sheets, and statement of consent forms. (DOCX 45 kb)
Lyricibracon Quicke
Creators: name: Ranjith, A. P.
name: Santhosh, S.
name: Nasser, M.
Publisher: name: Zenodo
Year: 2017
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hymenoptera
Braconidae
Lyricibracon

description: Key to species of Lyricibracon Quicke
1. Body largely black (Fig. 4), but frons (Fig. 4 A) and scutellum (Fig. 4 B) ivory white, and third metasomal tergite narrowly medially, anteriorly and posteriorly ivory white (Fig. 4 C); face transversely corrugated; mesoscutum smooth; second submarginal cell of fore wing long (Fig. 4 B); vein cu-a interstitial; hind basitarsus 7.1× as long as wide; second metasomal tergite longitudinally striate anteriorly; postero-lateral corners of sixth metasomal tergite pointed...... .. Lyricibracon bicolorus Quicke
– Body largely yellow (Figs 1–3) with frons (Fig. 1 B) and scutellum (Fig. 1 D) yellow but third metasomal tergite medio-anteriorly black (Fig. 1 E); face transversely striate posterior 1/3 rest rugose; mesoscutum transversely striate; second submarginal cell of fore wing short (Fig. 1 F); vein cu-a slightly postfurcal; hind basitarsus 4.6× as long as wide; postero-lateral corners of sixth metasomal tergite rounded............................................ Lyricibracon jenningsi Ranjith sp. nov.
description: Published as part of Ranjith, A. P., Santhosh, S. & Nasser, M., 2017, Range extension of the rare braconine genus, Lyricibracon Quicke (Hymenoptera: Braconidae), with the description of a new species from India, pp. 422-430 in Zootaxa 4227 (3) on page 423, DOI: 10.11646/zootaxa.4227.3.8, http://zenodo.org/record/268376
Additional file 1: Table S7. of The acceptability and feasibility of using the Adult Social Care Outcomes Toolkit (ASCOT) to inform practice in care homes
Creators: name: Ann-Marie Towers
name: Smith, Nick
name: Sinead Palmer
name: Welch, Elizabeth
name: Netten, Ann
Publisher: name: Figshare
Year: 2016
Subjects: subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Science Policy
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Summary of staff responses and any reported changes in practice. (DOCX 15 kb)
Additional file 2 of Time course of the response to ACTH in pig: biological and transcriptomic study
Creators: name: Sautron, Valérie
name: Terenina, Elena
name: Gress, Laure
name: Lippi, Yannick
name: Billon, Yvon
name: Larzul, Catherine
name: Liaubet, Laurence
name: Villa-Vialaneix, Nathalie
name: Mormède, Pierre
Publisher: name: Figshare
Year: 2015
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: Plant Biology
subject: Computational Biology

description: Distribution of the rank of the significant adjusted P -values in the tests for DE transcripts between t = 0 and t = + 1, t = 0 and t = + 4 and t = 0 and t = + 24 ‘.pdf’ file. P-values are smaller at t=+1 and t=+4 than at t=+24 implying that the transcripts were overall more differentially expressed between t=0 and t=+1 and between t=0 and t=+4 than between t=0 and t=+24. (PDF 4 kb)
Additional file 8: figure S8. of Biochemical and biophysical characterization of cell-free synthesized Rift Valley fever virus nucleoprotein capsids enables in vitro screening to identify novel antivirals
Creators: name: Broce, Sean
name: Hensley, Lisa
name: Tomoharu Sato
name: Lehrer-Graiwer, Joshua
name: Essrich, Christian
name: Edwards, Katie
name: Pajda, Jacqueline
name: Davis, Christopher
name: Bhadresh, Rami
name: Hurt, Clarence
name: Freeman, Beverly
name: Vishwanath Lingappa
name: Kelleher, Colm
name: Karpuj, Marcela
Publisher: name: Figshare
Year: 2016
Subjects: subject: Biochemistry
subject: Medicine
subject: Molecular Biology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: 60506 Virology

description: Specificity of various antibodies against RVFV NP tested by immunoprecipitation of the radiolabeled CFPS RVFV NP. (A) In all lanes, immunoprecipitated CFPS RVFV NPs were loaded, with the exception of lane 4, which represents the total material before immunoprecipitation (positive control), and lane 11, which represents the CFPS product of the Venezuelan Equine Encephalitis Virus NP (negative control). The following antibodies were tested: Lane 1, Monoclonal anti-native RVFV (see Additional file 1: figure S1); Lanes 2, 7, 8 and 9, four different rabbit polyclonal anti-RVFV antibodies; Lanes 3, 5 and 6, three different pig polyclonal anti-RVFV antibodies. The monoclonal antibody used for the immunoprecipitation reaction analyzed in lane 1, was also used in the high-throughput system (see text). (B) Optimization of the plate assay was performed comparing the purification of the capture Ab by ammonium persulfate (A-C, G-N) with the protein G purification (D-F, O, P), in 1:250 dilution (A-D, F-N, P) or 1:500 (E, O), whereas the biotinylated detection Ab was diluted to 1:126 (A-G, K-P) or to 1:250 (H). (PDF 113 kb)