title: ÐÐ¾Ð²ÑÐ¹ Ð¼ÐµÑÐ¾Ð´ Ð´ÐµÑÐµÐºÑÐ¸Ð¸ Ð¿Ð¾ÑÑÐ°Ð¿Ð½Ð¾Ð³Ð¾ ÑÐ¸Ð½ÑÐµÐ·Ð° ÐºÐ¾ÑÐ¾ÑÐºÐ¸Ñ Ð¿Ð¾Ð»Ð¸Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð²
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: New system for short polypeptides stepwise synthesis detection
Creators: name: ÐÐ¸Ð´Ð¶Ð¸ÐµÐ²Ð°, ÐÐµÐ´Ð¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: ÑÐ¸Ð±Ð¾ÑÐ¾Ð¼Ð°
ÑÑÐ°Ð½ÑÐ»ÑÑÐ¸Ñ
ÐºÐ¾ÑÐ¾ÑÐºÐ¸Ðµ Ð¿ÐµÐ¿ÑÐ¸Ð´Ñ
Ð³ÐµÐ»Ñ-ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÐ·
Ð°Ð½ÑÐ¸Ð±Ð¸Ð¾ÑÐ¸ÐºÐ¸
ribosome
translation
short peptides
gel electrophoresis
antibiotics

description: Ð¡ÑÑÐµÑÑÐ²ÑÐµÑ Ð½ÐµÑÐºÐ¾Ð»ÑÐºÐ¾ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð¿Ð¾ÑÑÐ°Ð¿Ð½Ð¾Ð³Ð¾ Ð±Ð¸Ð¾ÑÐ¸Ð½ÑÐµÐ·Ð° Ð±ÐµÐ»ÐºÐ°. ÐÐ´Ð½Ð°ÐºÐ¾ Ð±Ð¾Ð»ÑÑÐ¸Ð½ÑÑÐ²Ð¾ Ð¸Ð· Ð½Ð¸Ñ Ð¸Ð¼ÐµÑÑ ÑÑÐ´ Ð¾Ð³ÑÐ°Ð½Ð¸ÑÐµÐ½Ð¸Ð¹ Ð¸ Ð½ÐµÐ´Ð¾ÑÑÐ°ÑÐºÐ¾Ð². Ð Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÐµÐ½ Ð½Ð¾Ð²ÑÐ¹, Ð¿ÑÐ¾ÑÑÐ¾Ð¹ Ð¸ ÑÐ´Ð¾Ð±Ð½ÑÐ¹ Ð¸Ð½ÑÑÑÑÐ¼ÐµÐ½Ñ Ð´Ð»Ñ Ð¼Ð¾Ð½Ð¸ÑÐ¾ÑÐ¸Ð½Ð³Ð° Ð¿Ð¾ÑÑÐ°Ð¿Ð½Ð¾Ð¹ in vitro ÑÑÐ°Ð½ÑÐ»ÑÑÐ¸Ð¸ ÐºÐ¾ÑÐ¾ÑÐºÐ¸Ñ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð². ÐÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ðµ BODIPY-Ð¼ÐµÑÐµÐ½Ð¾Ð¹ Ð¸Ð½Ð¸ÑÐ¸Ð°ÑÐ¾ÑÐ½Ð¾Ð¹ Ð¼ÐµÑÐ¸Ð¾Ð½Ð¸Ð»-ÑÐ ÐÐ Ð² in vitro ÑÐ¸ÑÑÐµÐ¼Ðµ ÑÑÐ°Ð½ÑÐ»ÑÑÐ¸Ð¸ Ð¿Ð¾Ð·Ð²Ð¾Ð»Ð¸Ð»Ð¾ Ð²Ð¸Ð·ÑÐ°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°ÑÑ Ð¿Ð¾ÑÐ°Ð³Ð¾Ð²ÑÐ¹ ÑÐ¸Ð½ÑÐµÐ· Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð² Ð´Ð»Ð¸Ð½Ð¾Ð¹ Ð¾Ñ Ð¾Ð´Ð½Ð¾Ð¹ Ð´Ð¾ ÑÐµÐ¼Ð¸ Ð°Ð¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾Ñ Ð¿ÑÐ¸ Ð¿Ð¾Ð¼Ð¾ÑÐ¸ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐ°Ð·Ð´ÐµÐ»ÐµÐ½Ð¸Ñ Ð² Ð¿Ð¾Ð»Ð¸Ð°ÐºÑÐ¸Ð»Ð°Ð¼Ð¸Ð´Ð½Ð¾Ð¼ Ð³ÐµÐ»Ðµ Ð² Ð´ÐµÐ½Ð°ÑÑÑÐ¸ÑÑÑÑÐ¸Ñ ÑÑÐ»Ð¾Ð²Ð¸ÑÑ. ÐÐ¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾ÑÐ½Ð°Ñ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÑ Ð²Ð¸Ð·ÑÐ°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð² Ð±ÑÐ»Ð° Ð¿Ð¾Ð´ÑÐ²ÐµÑÐ¶Ð´ÐµÐ½Ð° Ð¿ÑÐ¸ Ð¿Ð¾Ð¼Ð¾ÑÐ¸ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½ÑÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ¾Ð² Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ ÑÐ°Ð´Ð¸Ð¾Ð°ÐºÑÐ¸Ð²Ð½Ð¾ Ð¼ÐµÑÐµÐ½Ð½ÑÑ Ð°Ð¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾Ñ.Â  ÐÐ¿Ð¸ÑÐ°Ð½Ð½ÑÐ¹ Ð¼ÐµÑÐ¾Ð´ Ð¿Ð¾Ð¼Ð¸Ð¼Ð¾ Ð¼Ð¾Ð½Ð¸ÑÐ¾ÑÐ¸Ð½Ð³Ð° ÑÐ°Ð·Ð½ÑÑ ÑÑÐ°Ð¿Ð¾Ð² ÑÑÐ°Ð½ÑÐ»ÑÑÐ¸Ð¸ ÑÐ°ÐºÐ¶Ðµ Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÐµÑ Ð¸Ð·ÑÑÐ°ÑÑ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ñ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾ÑÐ¾Ð² Ð±Ð¸Ð¾ÑÐ¸Ð½ÑÐµÐ·Ð° Ð±ÐµÐ»ÐºÐ°, ÑÑÐ¾ Ð±ÑÐ»Ð¾ Ð¿Ð¾Ð´ÑÐ²ÐµÑÐ¶Ð´ÐµÐ½Ð¾ Ð¿ÑÐ¸ Ð¿Ð¾Ð¼Ð¾ÑÐ¸ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½ÑÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ¾Ð² Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð°Ð½ÑÐ¸Ð±Ð¸Ð¾ÑÐ¸ÐºÐ¾Ð² Ñ Ð¸Ð·Ð²ÐµÑÑÐ½ÑÐ¼Ð¸ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð°Ð¼Ð¸ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ. Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð½ÑÐ¹ Ð¼ÐµÑÐ¾Ð´ Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÐµÑ ÑÐ°Ð·Ð´ÐµÐ»ÑÑÑ Ð¿Ð¾Ð»Ð¸Ð¿ÐµÐ¿ÑÐ¸Ð´Ñ Ð² Ð¿Ð¾Ð»Ð¸Ð°ÐºÑÐ¸Ð»Ð°Ð¼Ð¸Ð´Ð½Ð¾Ð¼ Ð³ÐµÐ»Ðµ Ñ Ð±ÐµÑÐ¿ÑÐµÑÐµÐ´ÐµÐ½ÑÐ½ÑÐ¼ ÑÐ°Ð·ÑÐµÑÐµÐ½Ð¸ÐµÐ¼ Ð² Ð¾Ð´Ð½Ñ Ð°Ð¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾ÑÑ. Ð¢Ð°ÐºÐ¶Ðµ ÑÐ½Ð¸ÐºÐ°Ð»ÑÐ½Ð¾Ð¹ Ð¾ÑÐ¾Ð±ÐµÐ½Ð½Ð¾ÑÑÑÑ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð½Ð¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ñ ÑÐ²Ð»ÑÐµÑÑÑ ÐµÐµ ÑÐ²ÐµÑÑÐ²ÑÑÐ¾ÐºÐ°Ñ ÑÑÐ²ÑÑÐ²Ð¸ÑÐµÐ»ÑÐ½Ð¾ÑÑÑ Ñ Ð¿Ð¾ÑÐ¾Ð³Ð¾Ð¼ Ð´ÐµÑÐµÐºÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð² Ð¾Ð±Ð»Ð°ÑÑÐ¸ 0,1 Ð¿Ð¼Ð¾Ð»Ñ Ð¿Ð¾Ð»Ð¸Ð¿ÐµÐ¿ÑÐ¸Ð´Ð°.
description: There are several methods for studying stepwise protein biosynthesis. However, most of them have limitations and drawbacks. In this work, we present a novel, simple and convenient tool for monitoring the stepwise in vitro translation of short polypeptides. The use of BODIPY-labeled initiator methionyl-tRNA in an in vitro translation system allowed us to detect the stepwise synthesis of short peptides (from one to seven amino acids in length) by electrophoretic separation in a polyacrylamide gel under denaturing conditions. The amino acid sequence of the visualized peptides was confirmed by control experiments using radioactively labeled amino acids.Â  In addition to monitoring different translation steps, the method also allows studying the mechanisms of action of protein biosynthesis inhibitors, which was confirmed by control experiments using antibiotics with known mechanisms of action. Developed method allows the separation of polypeptides in a polyacrylamide gel with an unprecedented resolution of one amino acid. Also, the unique feature of the developed system is its ultrahigh sensitivity with a detection threshold in the region of 0.1 pmol of polypeptide.