Additional file 1 of Methylation of serotonin regulating genes in cord blood cells: association with maternal metabolic parameters and correlation with methylation in peripheral blood cells during childhood and adolescence
Creators: name: Bečeheli, Ivona
name: Horvatiček, Marina
name: Perić, Maja
name: Nikolić, Barbara
name: Holuka, Cyrielle
name: Klasić, Marija
name: Ivanišević, Marina
name: Starčević, Mirta
name: Desoye, Gernot
name: Hranilović, Dubravka
name: Turner, Jonathan D.
name: Štefulj, Jasminka
Publisher: name: figshare
Year: 2024
Subjects: subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Physiology
subject: Pharmacology
subject: Developmental Biology
subject: Cancer
subject: Hematology

description: Additional file 1: Tables S1A–C. Associations between maternal metabolic parameters and other sample characteristics in PlaNS cohort. Table S1A. Association between maternal anthropometric parameters and continuous sample characteristics. Table S1B. Association between maternal anthropometric parameters and categorical sample characteristics. Table S1C. Association between maternal glucose tolerance status and other sample characteristics. Table S2. CpG sites targeted by methylation analyses in PlaNS cohort. Table S3. Methylation levels (%) at individual CpG sites in PlaNS participants. Table S4. Significance levels (p values) for bivariate associations of maternal and neonatal characteristics with methylation of serotonin regulating genes in cord blood cells of PlaNS participants. Table S5. Significance levels (p values) for correlation between cord blood cell type count and methylation of serotonin regulating genes in cord blood cells in a subset of PlaNS participants (N = 122). Tables S6A-S6E. Hierarchical linear regression analysis of maternal metabolic parameters as predictors of methylation at individual CpG sites in SLC6A4 gene in cord blood cells of PlaNS participants. Table S6A. Maternal metabolic parameters as predictors of methylation at CpG site #1 in SLC6A4 gene. Table S6B. Maternal metabolic parameters as predictors of methylation at CpG site #12 in SLC6A4 gene.Table S6C. Maternal metabolic parameters as predictors of methylation at CpG site #13 in SLC6A4 gene.Table S6D. Maternal metabolic parameters as predictors of methylation at CpG site #14 in SLC6A4 gene. Table S6E. Maternal metabolic parameters as predictors of methylation at CpG site #15 in SLC6A4 gene.Table S7. Methylation levels (β values) in ARIES participants at CpG sites overlapping with CpG sites analyzed in PlaNS cohort. Table S8. Correlation between SLC6A4 methylation and expression in cord blood cells. Table S9. Primers used in DNA methylation analysis by bisulfite pyrosequencing in PlaNS cohort. Figure S1. Correlation between methylation levels at CpG sites in (A) SLC6A4 and (B) MAOA, as observed in the PlaNS cohort. Figure S2. Association of newborn sex and maternal pre-pregnancy body weight status with methylation of SLC6A4 and HTR2A CpG sites in cord blood monocytes, as determined in GSE212174 dataset [49]. Figure S3. Association of newborn sex and maternal glucose tolerance status with methylation of SLC6A4 and HTR2A CpG sites in cord blood cells, as determined in GSE141065 dataset [50]. Figure S4. Correlation between methylation levels at CpG sites in A SLC6A4 and B HTR2A, as observed in the ARIES cohort. Figure S5. Temporal mapping of methylation quantitative trait loci (mQTLs) for Illumina 450K CpG sites in A SLC6A4 and B HTR2A genes in the ARIES cohort.