cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Where Does Evidence Come From?: Crowd-Sourced Digital Archive DH/CBL Project for Students at Two-Year Institutions
Creators: name: Sanders, Laura
Publisher: name: unknown
Year: 2015
Subjects: subject: Casual labor
subject: Digital humanities
subject: Experiential learning
subject: Teaching

description: Combining my passions for professional development and social justice, I continue to seek the sweet spot between digital humanities and online community-based learning. DH is particularly exciting for students at two-year, open access institutions because it has the potential to level the playing field as well as to give under-represented communities a voice and the opportunity to offer their own narratives to counter those presented in dominant culture. In this assignment, I ask my writing students to consider more deeply how knowledge is constructed and on whose terms through a reflection paper on their experiences with crowd-sourced digital archives.
Brincidofovir clearance of acyclovir-resistant herpes simplex virus-1 and adenovirus infection after stem cell transplantation
Creators: name: Voigt, Sebastian
name: Hofmann, Jörg
name: Edelmann, Anke
name: Sauerbrei, Andreas
name: Kühl, J. S.
Publisher: name: Robert Koch-Institut, Infektionskrankheiten / Erreger
Year: 2016
Subjects: subject: Viral/blood
subject: Humans
subject: Female
subject: DNA
subject: Child Preschool
subject: Drug Resistance Viral
subject: Viral Load
subject: Antiviral Agents/therapeutic use
subject: Immunocompromised Host
subject: Acyclovir/administration & dosage
subject: Acyclovir/therapeutic use
subject: Adenoviridae/isolation & purification
subject: Adenoviridae/physiology
subject: Adenovirus Infections Human/blood
subject: Adenovirus Infections Human/drug therapy
subject: Adenovirus Infections Human/virology
subject: Antibiotic Prophylaxis
subject: Antiviral Agents/administration & dosage
subject: Cytosine/administration & dosage
subject: Cytosine/analogs & derivatives
subject: Cytosine/therapeutic use
subject: Epstein-Barr Virus Infections/blood
subject: Epstein-Barr Virus Infections/drug therapy
subject: Epstein-Barr Virus Infections/virology
subject: Foscarnet/administration & dosage
subject: Foscarnet/therapeutic use
subject: Hematopoietic Stem Cell Transplantation/adverse effects
subject: Herpes Simplex/drug therapy
subject: Herpes Simplex/virology
subject: Herpes Zoster/drug therapy
subject: Herpes Zoster/virology
subject: Herpesvirus 1 Human/isolation & purification
subject: Herpesvirus 3 Human/isolation & purification
subject: Herpesvirus 4 Human
subject: Meningoencephalitis/drug therapy
subject: Meningoencephalitis/virology
subject: Mucositis/drug therapy
subject: Mucositis/virology
subject: Myelodysplastic Syndromes/surgery
subject: Organophosphonates/administration & dosage
subject: Organophosphonates/therapeutic use
subject: Rituximab/administration & dosage
subject: Rituximab/therapeutic use
subject: 610 Medizin

description: Infections with adenovirus (AdV) and herpesviruses can result in considerable morbidity and mortality in pediatric hematopoietic stem cell transplant (SCT) recipients. Herpes simplex virus (HSV) reactivations are usually prevented by acyclovir (ACV) prophylaxis, whereas cidofovir (CDV) has been used off indication to manage AdV infections. We report a child with myelodysplastic syndrome undergoing multiple SCT, who experienced HSV-1 disease including severe mucositis and herpetic whitlow, as well as high viral load AdV DNAemia. Both ACV and CDV were ineffective; however, viral loads were decreased with brincidofovir, resulting in viral clearance. A subsequent Epstein–Barr virus disease with relevant meningoencephalitis responded to rituximab.
Acute childhood diarrhoea in northern Ghana: epidemiological, clinical and microbiological characteristics
Creators: name: Reither, Klaus
name: Ignatius, Ralf
name: Weitzel, Thomas
name: Seidu-Korkor, Andrew
name: Anyidoho, Louis
name: Saad, Eiman
name: Djie-Maletz, Andrea
name: Ziniel, Peter
name: Amoo-Sakyi, Felicia
name: Danikuu, Francis
name: Danour, Stephen
name: Otchwemah, Rowland N.
name: Schreier, Eckart
name: Bienzle, Ulrich
name: Stark, Klaus
name: Mockenhaupt, Frank P.
Publisher: name: Robert Koch-Institut, Infektionskrankheiten / Erreger
Year: 2007
Subjects: subject: Humans
subject: Child
subject: Preschool
subject: Infant
subject: Questionnaires
subject: Feces/virology
subject: Anti-Infective Agents/therapeutic use
subject: hild
subject: Diarrhea/epidemiology
subject: Diarrhea/therapy
subject: Diarrhea/virology
subject: Fluid Therapy
subject: Ghana/epidemiology
subject: Logistic Models
subject: Rotavirus/isolation & purification
subject: Rotavirus Infections/epidemiology
subject: Rotavirus Infections/therapy
subject: Rotavirus Infections/virology
subject: Urban Population
subject: 610 Medizin

description: Background: Acute diarrhoea is a major cause of childhood morbidity and mortality in sub-Saharan Africa. Its microbiological causes and clinico-epidemiological aspects were examined during the dry season 2005/6 in Tamale, urban northern Ghana. Methods: Stool specimens of 243 children with acute diarrhoea and of 124 control children were collected. Patients were clinically examined, and malaria and anaemia were assessed. Rota-, astro-, noro- and adenoviruses were identified by (RT-) PCR assays. Intestinal parasites were diagnosed by microscopy, stool antigen assays and PCR, and bacteria by culturing methods. Results: Watery stools, fever, weakness, and sunken eyes were the most common symptoms in patients (mean age, 10 months). Malaria occurred in 15% and anaemia in 91%; underweight (22%) and wasting (19%) were frequent. Intestinal micro-organisms were isolated from 77% of patients and 53% of controls (P < 0.0001). The most common pathogens in patients were rotavirus (55%), adenovirus (28%) and norovirus (10%); intestinal parasites (5%) and bacteria (5%) were rare. Rotavirus was the only pathogen found significantly more frequently in patients than in controls (odds ratio 7.7; 95%CI, 4.2–14.2), and was associated with young age, fever and watery stools. Patients without an identified cause of diarrhoea more frequently had symptomatic malaria (25%) than those with diagnosed intestinal pathogens (12%, P = 0.02). Conclusion: Rotavirus-infection is the predominant cause of acute childhood diarrhoea in urban northern Ghana. The abundance of putative enteropathogens among controls may indicate prolonged excretion or limited pathogenicity. In this population with a high burden of diarrhoeal and other diseases, sanitation, health education, and rotavirus-vaccination can be expected to have substantial impact on childhood morbidity.
Additional file 1 of Physiological oxygen conditions enhance the angiogenic properties of extracellular vesicles from human mesenchymal stem cells
Creators: name: Phelps, Jolene
name: Hart, David A.
name: Mitha, Alim P.
name: Duncan, Neil A.
name: Sen, Arindom
Publisher: name: figshare
Year: 2024
Subjects: subject: Biochemistry
subject: Space Science
subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Physiology
subject: Pharmacology
subject: Developmental Biology
subject: Cancer
subject: Hematology

description: Additional file 1. Table S1 Concentrations of angiogenic proteins in EV fractions from normoxic (18.4% O2) and physioxic (3% O2) MSC cultures.
Digital Narratives syllabus (undergrad media writing course)
Creators: name: Trettien, Whitney
Publisher: name: Humanities Commons
Year: 2017
Subjects: subject: Digital humanities
subject: Mass media--Study and teaching

description: The attached syllabus was written for my upper-level undergraduate seminar "Digital Narratives," taught Spring 2013 at Duke University. Here is an excerpt from the course description: "This course considers what it means to tell stories in an age of digital media. We’ll begin by writing a traditional short story (fiction or creative non-fiction), focusing on plot and structure. We’ll then experiment with “translating” this narrative into a variety of new media forms. How does your story change when told as an interactive fiction? as a video game? as a hypertext novel? or on different platforms like Twitter, YouTube, Facebook or Second Life? With each “translation,” we’ll read relevant texts on narratology and media theory as a way of giving us a shared vocabulary for discussing these new genres, and we’ll explore some of the best examples of creative writing in them."
Understanding rural (dis)content - methodological approach for the project 'Social and Political Consequences of Spatial Inequality'
Creators: name: Bischof, Susann
name: Lengerer, Franziska
name: Deppisch, Larissa
name: Decker, Anja
name: Władyniak, Ludmiła Dobrovolná
name: Konopski, Michał
name: Grzelak, Anna
name: Sidarava, Aliaksandra
name: Bernard, Josef
name: Klärner, Andreas
Publisher: name: Unknown
Year: 2025
Subjects: subject: Institutional and Behavioral Economics
subject: Research Research Methods/Statistical Methods
subject: left behind places
subject: geography of discontent
subject: rural areas
subject: qualitative methods
subject: focus group
subject: expert interviews
subject: visual methods
subject: thematic analysis
subject: methodology

description: The prevailing socio-economic disparities within numerous European nations are becoming increasingly pro-nounced. However, our understanding of their social and political implications, particularly with respect to the rise of populism and the sentiment of being ‘left behind’, remains limited. The project, titled ‘Social and Political Consequences of Spatial Inequalities: A Case Study of Central-Eastern Europe’ (SPC Spatial) aims to address these issues through empirical verification, international comparison, a focus on East-Central Europe, and a mixed-method approach. This Working Paper is part of the project’s qualitative work package, which specifically targets the perceptions and perspectives of people living in disadvantaged regions. It aims to understand how different age and income groups perceive their local living conditions and how this shapes their future prospects and their relationship to politics. To enhance the transparency of our research, this methodological working paper presents in detail our basic methodological assumptions and the empirical approach, which includes ex-pert interviews, focus groups, the use of visual stimuli, and a thematic analysis of the data.
description: 10.3220/253-2025-53
Additional file 1 of Patient interest in the development of a center for Ehlers-Danlos syndrome/hypermobility spectrum disorder in the Chicagoland region
Creators: name: Wagner, Wendy
name: Doyle, Tom A.
name: Francomano, Clair A.
name: Knight, Dacre R. T.
name: Halverson, Colin M. E.
Publisher: name: figshare
Year: 2024
Subjects: Medicine
Biotechnology
Cancer
Science Policy
Mental Health

description: Additional file 1: Utilization of medical disciplines.
Digital Narratives syllabus (undergrad media writing course)
Creators: name: Trettien, Whitney
Publisher: name: unknown
Year: 2017
Subjects: subject: Mass media--Study and teaching
subject: Digital humanities

description: The attached syllabus was written for my upper-level undergraduate seminar "Digital Narratives," taught Spring 2013 at Duke University. Here is an excerpt from the course description: "This course considers what it means to tell stories in an age of digital media. We'll begin by writing a traditional short story (fiction or creative non-fiction), focusing on plot and structure. We'll then experiment with "translating" this narrative into a variety of new media forms. How does your story change when told as an interactive fiction? as a video game? as a hypertext novel? or on different platforms like Twitter, YouTube, Facebook or Second Life? With each "translation," we'll read relevant texts on narratology and media theory as a way of giving us a shared vocabulary for discussing these new genres, and we'll explore some of the best examples of creative writing in them."
Scrapie Agent (Strain 263K) Can Transmit Disease via the Oral Route after Persistence in Soil over Years
Creators: name: Seidel, Bjoern
name: Thomzig, Achim
name: Buschmann, Anne
name: Groschup, Martin H.
name: Peters, Rainer
name: Beekes, Michael
name: Terytze, Konstantin
Publisher: name: Robert Koch-Institut, Infektionskrankheiten / Erreger
Year: 2007
Subjects: subject: Animals
subject: Western
subject: Cricetinae
subject: Biological Assay
subject: Blotting
subject: PrPSc Proteins/isolation & purification
subject: PrPSc Proteins/pathogenicity
subject: Soil
subject: 610 Medizin

description: The persistence of infectious biomolecules in soil constitutes a substantial challenge. This holds particularly true with respect to prions, the causative agents of transmissible spongiform encephalopathies (TSEs) such as scrapie, bovine spongiform encephalopathy (BSE), or chronic wasting disease (CWD). Various studies have indicated that prions are able to persist in soil for years without losing their pathogenic activity. Dissemination of prions into the environment can occur from several sources, e.g., infectious placenta or amniotic fluid of sheep. Furthermore, environmental contamination by saliva, excrements or non-sterilized agricultural organic fertilizer is conceivable. Natural transmission of scrapie in the field seems to occur via the alimentary tract in the majority of cases, and scrapie-free sheep flocks can become infected on pastures where outbreaks of scrapie had been observed before. These findings point to a sustained contagion in the environment, and notably the soil. By using outdoor lysimeters, we simulated a contamination of standard soil with hamster-adapted 263K scrapie prions, and analyzed the presence and biological activity of the soil-associated PrPSc and infectivity by Western blotting and hamster bioassay, respectively. Our results showed that 263K scrapie agent can persist in soil at least over 29 months. Strikingly, not only the contaminated soil itself retained high levels of infectivity, as evidenced by oral administration to Syrian hamsters, but also feeding of aqueous soil extracts was able to induce disease in the reporter animals. We could also demonstrate that PrPSc in soil, extracted after 21 months, provides a catalytically active seed in the protein misfolding cyclic amplification (PMCA) reaction. PMCA opens therefore a perspective for considerably improving the detectability of prions in soil samples from the field.
Networked and Multimodal Composition syllabus (undergrad course)
Creators: name: Trettien, Whitney
Publisher: name: unknown
Year: 2017
Subjects: subject: Digital humanities

description: The attached syllabus was written for a 100-level undergraduate seminar "Networked and Multimodal Composition," taught Fall 2015 at UNC Chapel Hill. The course objectives: "This course aims to empower you, the student, with the tools, skills, and critical vocabulary necessary to compose sophisticated and meaningful digital compositions. By the end of this course, you will be able to:
• tell stories effectively using text, image, audio, video, physical space, interactive systems, and social media platforms;
• discuss and critically analyze digital technologies and their impact on how we communicate ideas;
• plan, design, build, and publish your own multimodal projects, from start to finish."
description: The attached syllabus was written for a 100-level undergraduate seminar "Networked and Multimodal Composition," taught Fall 2015 at UNC Chapel Hill. The course objectives: "This course aims to empower you, the student, with the tools, skills, and critical vocabulary necessary to compose sophisticated and meaningful digital compositions. By the end of this course, you will be able to: • tell stories effectively using text, image, audio, video, physical space, interactive systems, and social media platforms; • discuss and critically analyze digital technologies and their impact on how we communicate ideas; • plan, design, build, and publish your own multimodal projects, from start to finish."
In Deutschland selten auftretende Infektionskrankheiten – Ergebnisse aus der Meldepflicht.
Creators: name: Schöneberg, Irene
Publisher: name: Robert Koch-Institut, Infektionsepidemiologie
Year: 2008
Subjects: subject: Seltene Infektionskrankheiten
subject: Infektionsschutzgesetz
subject: Meldepflichtige Krankheiten
subject: Meldepflicht
subject: rare infectious diseases
subject: Protection against Infection Act
subject: notifiable diseases
subject: obligatory notifications
subject: 610 Medizin

description: Selten auftretende Infektionskrankheiten haben in Deutschland eine große Bedeutung: Einige von ihnen erfordern bei ihrem Auftreten besondere Maßnahmen und Vorkehrungen, um eine Weiterverbreitung zu verhindern (z. B. virusbedingte hämorrhagische Fieber). Bei anderen stehen Präventionsaspekte im Vordergrund (impfpräventable Krankheiten). Zusammenfassende Darstellungen für einzelne seltene Krankheiten, die die Situation in Deutschland insgesamt beschreiben, sind nur auf Grundlage der Meldepflicht möglich. Die gesetzliche Meldepflicht ist eine gute Voraussetzung, um in Deutschland auftretende Krankheiten zu erfassen. Mit Einführung des Infektionsschutzgesetzes (IfSG) 2001 verbesserte sich die Qualität der verfügbaren Daten durch die Anwendung von Falldefinitionen. Die Auswertung der vorliegenden Meldungen zeigt: Auf der Grundlage der vorhandenen Daten ergeben sich keine Anhaltspunkte dafür, dass sich die Gefährdung durch einzelne seltene Krankheiten in Deutschland in absehbarer Zeit spürbar erhöhen könnte.
description: Rare infectious diseases are of great importance in Germany: Some of them require special measures and precautions to prevent spreading (e.g. virus caused hemorrhagic fevers). For others, e.g. infectious diseases that can be prevented by vaccination, the prevention aspects are important. Summarizing descriptions for several rare diseases which together describe the situation in Germany are only possible on the basis of the notification requirements. The legal obligation for notification represents a good prerequisite to register diseases occurring in Germany. With the introduction of the Protection against Infection Act in 2001 the quality of the available data was improved by using case definitions. The evaluation of existing information indicates: On the basis of the available data there is no evidence that in the foreseeable future a noticeable increase of isolated rare diseases could pose a threat in Germany.
Networked and Multimodal Composition syllabus (undergrad course)
Creators: name: Trettien, Whitney
Publisher: name: Humanities Commons
Year: 2017
Subjects: subject: Digital humanities

description: The attached syllabus was written for a 100-level undergraduate seminar “Networked and Multimodal Composition,” taught Fall 2015 at UNC Chapel Hill. The course objectives: "This course aims to empower you, the student, with the tools, skills, and critical vocabulary necessary to compose sophisticated and meaningful digital compositions. By the end of this course, you will be able to: • tell stories effectively using text, image, audio, video, physical space, interactive systems, and social media platforms; • discuss and critically analyze digital technologies and their impact on how we communicate ideas; • plan, design, build, and publish your own multimodal projects, from start to finish."
Additional file 1 of Adaptation and feasibility assessment of a dating violence prevention program for girls in foster care
Creators: name: de Moura, Julliane Quevedo
name: Saratt, Manoela Mosena
name: da Silva, Stephanie Caroline Souza
name: Silva, Victória Caroline
name: Murta, Sheila Giardini
name: Habigzang, Luísa Fernanda
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Sociology
subject: FOS: Sociology
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Science Policy
subject: Infectious Diseases
subject: FOS: Health sciences

description: Supplementary Material 1.
Accumulation of Pathological Prion Protein PrPSc in the Skin of Animals with Experimental and Natural Scrapie
Creators: name: Thomzig, Achim
name: Schulz-Schaeffer, Walter
name: Wrede, Arne
name: Wemheuer, Wilhelm
name: Brenig, Bertram
name: Kratzel, Christine
name: Lemmer, Karin
name: Beekes, Michael
Publisher: name: Robert Koch-Institut, Infektionskrankheiten / Erreger
Year: 2007
Subjects: subject: Animals
subject: Cricetinae
subject: Neurons
subject: PrPSc Proteins/analysis
subject: PrPSc Proteins/metabolism
subject: Scrapie/pathology
subject: Skin/chemistry
subject: kin/pathology
subject: Tissue Distribution
subject: 610 Medizin

description: Prion infectivity and its molecular marker, the pathological prion protein PrPSc, accumulate in the central nervous system and often also in lymphoid tissue of animals or humans affected by transmissible spongiform encephalopathies. Recently, PrPSc was found in tissues previously considered not to be invaded by prions (e.g., skeletal muscles). Here, we address the question of whether prions target the skin and show widespread PrPSc deposition in this organ in hamsters perorally or parenterally challenged with scrapie. In hamsters fed with scrapie, PrPSc was detected before the onset of symptoms, but the bulk of skin-associated PrPSc accumulated in the clinical phase. PrPSc was localized in nerve fibres within the skin but not in keratinocytes, and the deposition of PrPSc in skin showed no dependence from the route of infection and lymphotropic dissemination. The data indicated a neurally mediated centrifugal spread of prions to the skin. Furthermore, in a follow-up study, we examined sheep naturally infected with scrapie and detected PrPSc by Western blotting in skin samples from two out of five animals. Our findings point to the skin as a potential reservoir of prions, which should be further investigated in relation to disease transmission.
Online supplementary file 1 - Supplemental material for Towards Smarter and Fairer Justice? A Review of the Chinese Scholarship on Building Smart Courts and Automating Justice
Creators: name: Papagianneas, Straton
Publisher: name: SAGE Journals
Year: 2021
Subjects: subject: 120505 Regional Analysis and Development
subject: FOS: Social and economic geography

description: Supplemental material, Online supplementary file 1, for Towards Smarter and Fairer Justice? A Review of the Chinese Scholarship on Building Smart Courts and Automating Justice by and Straton Papagianneas in Journal of Current Chinese Affairs
Urban-rural disparities in smoking behaviour in Germany
Creators: name: Völzke, Henry
name: Neuhauser, Hannelore
name: Moebus, Susanne
name: Baumert, Jens
name: Berger, Klaus
name: Stang, Andreas
name: Ellert, Ute
name: Werner, André
name: Döring, Angela
Publisher: name: Robert Koch-Institut, Epidemiologie und Gesundheitsberichterstattung
Year: 2006
Subjects: subject: Aged
subject: Humans
subject: Female
subject: Germany/epidemiology
subject: Male
subject: Middle Aged
subject: Prevalence
subject: Child
subject: * Adolescent
subject: * Adult
subject: * Aged
subject: Family Characteristics
subject: Health Behavior
subject: Health Surveys
subject: Interviews as Topic
subject: Odds Ratio
subject: Residence Characteristics/classification
subject: Residence Characteristics/statistics & numerical data
subject: Risk-Taking
subject: Rural
subject: Population/statistics & numerical data
subject: Smoking/epidemiology
subject: Smoking/psychology
subject: Urban Population/statistics & numerical data
subject: Aged 80 and over
subject: 610 Medizin

description: Background: It is currently not clear whether individuals living in metropolitan areas differ from individuals living in rural and urban areas with respect to smoking behaviours. Therefore, we sought to explore the relation between residential area and smoking behaviours in Germany. Methods: We used a nationwide German census representative for the general population of Germany. A number of 181,324 subjects aged 10 years or older were included. Information on the average daily usage of cigarettes that have or had been smoked formerly or currently was available in subjects who have ever smoked. A daily consumption of more than 20 cigarettes was considered heavy smoking. Logistic regression analyses were performed sex-stratified and adjusted for relevant confounders.Results: Analyses revealed inhabitants of metropolitan areas to be more likely current smokers than inhabitants of rural areas (odds ratio 1.56, 95%-confidence interval 1.51; 1.62). Among current and former smokers those who lived in urban communities had also increased odds for being heavy smokers than those who lived in rural communities. Conclusion: We conclude that living in an urban and particularly living in a metropolitan area is a determinant of both smoking and severity of current smoking. Tobacco control programs should recognize the difference in living conditions between rural and urban areas.
The Art of the Book in the Digital Age syllabus (undergraduate course)
Creators: name: Trettien, Whitney
Publisher: name: Humanities Commons
Year: 2017
Subjects: subject: Digital humanities

description: The attached syllabus was written for my Honors undergraduate seminar “The Art of the Book in the Digital Age,” taught Fall 2016 at UNC Chapel Hill. Here is an excerpt from the course description: "The book’s role and significance within literary culture is being scrutinized today with an intensity unseen for five centuries. Nowhere is this questioning more acute, sophisticated, and nuanced than in the burgeoning field of the book arts, an umbrella term encompassing artists’ books, book sculpture, zines, and print-oriented forms of electronic poetry. This is an inherently collaborative and interdisciplinary field. Its practitioners skirt the thresholds between visual art and literature, technology and philosophy, producing uniquely bookish artifacts that defy easy categorization. These are artworks made not for the white walls of a gallery, but to be read and used; they are works of literature that engage the visual, tactile, and even olfactory senses. Difficult to reproduce in print editions or literary anthologies, they challenge our expectations of the codex as a platform for delivering and consuming textual information. Despite the diversity of the book arts, what brings these practices together is a shared interest in the potential of the book to model radical new forms of creativity, subjectivity, and political engagement. 'if i can sing through my mouth with a book,' writes El Lissitzky in a treatise on book design, 'i can show myself in various guises.'"
Additional file 1 of Desmocollin-1 is associated with pro-metastatic phenotype of luminal A breast cancer cells and is modulated by parthenolide
Creators: name: Lapcik, Petr
name: Sulc, Petr
name: Janacova, Lucia
name: Jilkova, Katerina
name: Potesil, David
name: Bouchalova, Pavla
name: Müller, Petr
name: Bouchal, Pavel
Publisher: name: figshare
Year: 2024
Subjects: subject: Biophysics
subject: Biochemistry
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Cancer
subject: Hematology
subject: Plant Biology

description: Additional file 1: Fig. S1. Western blot analysis of DSC1 modulation using inhibitors niclosamide, norcantharidin and parthenolide. (A) processed images, (B) raw image of immunoblotting with anti-DSC1 antibody, (C) raw image of immunoblotting with anti-ACTB antibody. Tab. S1 Statistical evaluation of semiquantitative analysis of effect of inhibitors on protein levels of DSC1 longer and shorter isoforms. Fig. S2 (A) Representative chromatograms of iRT peptides measured in the total proteome experiment displayed in Skyline software, (B) iRT calibration chart used in the directDIA analysis in Spectronaut software. Tab. S2 Proteins significantly up-regulated after DSC1 overexpression. Fig. S3 Western blot verification of SBP tag presence in control MCF7-GFP cells and MCF7-DSC1-GFP cells used for pull-down identification of DSC1 protein interaction partners. Antibodies: Anti-SBP-tag (left), Anti-DSC1 (right) and Anti-ACTB (down).
The Art of the Book in the Digital Age syllabus (undergraduate course)
Creators: name: Trettien, Whitney
Publisher: name: unknown
Year: 2017
Subjects: subject: Digital humanities

description: The attached syllabus was written for my Honors undergraduate seminar "The Art of the Book in the Digital Age," taught Fall 2016 at UNC Chapel Hill. Here is an excerpt from the course description: "The book's role and significance within literary culture is being scrutinized today with an intensity unseen for five centuries. Nowhere is this questioning more acute, sophisticated, and nuanced than in the burgeoning field of the book arts, an umbrella term encompassing artists' books, book sculpture, zines, and print-oriented forms of electronic poetry. This is an inherently collaborative and interdisciplinary field. Its practitioners skirt the thresholds between visual art and literature, technology and philosophy, producing uniquely bookish artifacts that defy easy categorization. These are artworks made not for the white walls of a gallery, but to be read and used; they are works of literature that engage the visual, tactile, and even olfactory senses. Difficult to reproduce in print editions or literary anthologies, they challenge our expectations of the codex as a platform for delivering and consuming textual information. Despite the diversity of the book arts, what brings these practices together is a shared interest in the potential of the book to model radical new forms of creativity, subjectivity, and political engagement. 'if i can sing through my mouth with a book,' writes El Lissitzky in a treatise on book design, 'i can show myself in various guises.'"
Additional file 1 of Investigating the effect of a school-based WASH intervention on soil-transmitted helminth and schistosome infections and nutritional status of school children in Ethiopia: a quasi-experimental study
Creators: name: Tadesse, Gemechu
name: Wuletaw, Yonas
name: Mekete, Kalkidan
name: Sime, Heven
name: Yard, Elodie
name: Appleby, Laura
name: Grimes, Jack
name: Dejene, Nigussie
name: Gardiner, Iain
name: Kazienga, Adama
name: Abbeddou, Souheila
name: French, Michael
name: Levecke, Bruno
name: Drake, Lesley
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Environmental Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Science Policy
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Computational Biology

description: Additional file 1: Table S1. The demographic characteristics, status of helminth infections and nutritional status at baseline compared between age categories.
Technologies of Literary Production (grad course, taught Spring 2017)
Creators: name: Trettien, Whitney
Publisher: name: Humanities Commons
Year: 2017
Subjects: subject: Digital humanities

description: The attached syllabus was written for my Spring 2017 graduate course "Technologies of Literary Production." From the course description: "This course has two complementary goals. The first is to introduce the history of technologies used to produce and circulate literature, from the parchment upon which Beowulf is written to the social media platforms exploited by netprov artists. This history provides a broad overview of the material conditions of possibility for the emergence of literary form and genre in the Anglophone tradition. The second goal is to examine how digital media are transforming scholarly publishing and communication by reflecting upon our own writing practices and their attendant technologies. By pursuing these two goals in tandem, this course places current trends, like digital humanities, within a much longer history of technological transformation and textual production."
Inanspruchnahme außerfamiliärer vorschulischer Kindertagesbetreuung und Einfluss auf Merkmale psychischer Gesundheit bei Kindern
Creators: name: Schlack, Robert
name: Hölling, Heike
name: Kurth, Bärbel-Maria
Publisher: name: Robert Koch-Institut
Year: 2007
Subjects: subject: Kinder- und Jugendgesundheitssurvey
subject: SDQ
subject: Außerfamiliäre Betreuung im Vorschulalter
subject: Soziale Kompetenz
subject: Verhaltensauffälligkeiten
subject: 610 Medizin

description: Im Kinder- und Jugendgesundheitssurvey (KiGGS), einer repräsentativen Gesundheitsstudie für Kinder und Jugendliche der Bundesrepublik Deutschland mit 17.641 Teilnehmern im Alter von 0–17 Jahre wurde u. a. die Betreuungssituation im Vorschulalter erfasst. Die Daten lassen Aussagen darüber zu, wie viele Kinder außerfamiliär betreut wurden und welche Betreuungsform (Kinderkrippe/KiTa/Kindergarten; Tagesmutter) vorlag. Dabei kann nach sozioökonomischen Kategorien wie höchster Bildungsabschluss und Berufstätigkeit der Mutter, Vollständigkeit der Familie, Migration und Herkunft Ost/West differenziert werden. Die hier vorgestellte Arbeit soll einen Beitrag zur Diskussion über die Konsequenzen vorschulischer Kindertagesbetreuung leisten und geht der Frage nach, ob und inwieweit sich ausschließlich familiär betreute Kinder hinsichtlich Verhaltensauffälligkeiten und prosozialem Verhalten von denen unterscheiden, die in einer Kindertageseinrichtung betreut werden und welche Rahmenbedingungen die Inanspruchnahme einer Kindertagesbetreuung befördern. Insgesamt 74,7 % der Kinder und Jugendlichen befanden sich zum Befragungszeitpunkt in außerfamiliär vor schulischer Tagesbetreuung oder haben diese jemals in Anspruch genommen. Große Unterschiede bestehen nach wie vor zwischen Ost- und Westdeutschland. Ostdeutsche Kinder befinden sich weitaus mehr und früher in vorschulischer Tagesbetreuung als westdeutsche Kinder. Außerfamiliär betreute Kinder von Müttern mit Universitäts-/Fachhochschulabschluss kommen zu einem wesentlich früheren Zeitpunkt in die Tagesbetreuung als Kinder von Müttern mit niedrigeren Bildungsabschlüssen. Kinder von Müttern mit Vollzeitbeschäftigung und Kinder Alleinerziehender befinden sich ebenfalls häufiger und früher in vorschulischer Tagesbetreuung, Kinder mit Migrationshintergrund hingegen insgesamt weniger und später. Die mulitvariate Betrachtung der verschiedenen Einflussfaktoren im logistischen Modell erbrachte keine Hinweise auf ein erhöhtes Risiko für psychische Auffälligkeiten (erfasst mit dem SDQ) von Kindern, die sich zum Befragungszeitpunkt in vorschulischer Tagesbetreuung befanden. Dabei wurde auch das Alter bei Betreuungsbeginn berücksichtigt. Es gab keine Hinweise auf Unterschiede im prosozialen Verhalten der Kinder in Abhängigkeit vom Besuch vorschulischer Kinderbetreuungsstätten. Mit den in dieser Arbeit dargestellten Ergebnissen soll die Diskussion um Vor- bzw. Nachteile einer außerfamiliären Kindertagesbetreuung weiter befördert und versachlicht werden. Ziel ist es dabei, die Bedingungen für die bestmöglichen Entwicklungschancen der Kinder in Deutschland zu eruieren.
Technologies of Literary Production (grad course, taught Spring 2017)
Creators: name: Trettien, Whitney
Publisher: name: unknown
Year: 2017
Subjects: subject: Digital humanities

description: The attached syllabus was written for my Spring 2017 graduate course "Technologies of Literary Production." From the course description: "This course has two complementary goals. The first is to introduce the history of technologies used to produce and circulate literature, from the parchment upon which Beowulf is written to the social media platforms exploited by netprov artists. This history provides a broad overview of the material conditions of possibility for the emergence of literary form and genre in the Anglophone tradition. The second goal is to examine how digital media are transforming scholarly publishing and communication by reflecting upon our own writing practices and their attendant technologies. By pursuing these two goals in tandem, this course places current trends, like digital humanities, within a much longer history of technological transformation and textual production."
Human Coronavirus NL63 Open Reading Frame 3 encodes a virion-incorporated N-glycosylated membrane protein
Creators: name: Müller, Marcel A.
name: Hoek, Lia Van Der
name: Voss, Daniel
name: Bader, Oliver
name: Lehmann, Dörte
name: Schulz, Axel R.
name: Kallies, Stephan
name: Suliman, Tasnim
name: Fielding, Burtram C
name: Drosten, Christian
name: Niedrig, Matthias
Publisher: name: Robert Koch-Institut
Year: 2010
Subjects: subject: Animals
subject: Western
subject: Molecular
subject: Cell Line
subject: Humans
subject: Microscopy
subject: Models
subject: Biological
subject: Macaca mulatta
subject: Blotting
subject: Coronavirus/genetics
subject: Endoplasmic Reticulum/chemistry
subject: Glycoproteins/genetics
subject: Glycoproteins/metabolism
subject: Golgi Apparatus/chemistry
subject: Lysosomes/chemistry
subject: Confocal
subject: Fluorescence
subject: Open Reading Frames
subject: Viral Matrix Proteins/genetics
subject: Viral Matrix Proteins/metabolism
subject: Viral Structural Proteins/analysis
subject: Virion/chemistry
subject: 610 Medizin

description: Background: Human pathogenic coronavirus NL63 (hCoV-NL63) is a group 1 (alpha) coronavirus commonly associated with respiratory tract infections. In addition to known non-structural and structural proteins all coronaviruses have one or more accessory proteins whose functions are mostly unknown. Our study focuses on hCoV-NL63 open reading frame 3 (ORF 3) which is a highly conserved accessory protein among coronaviruses. Results: In-silico analysis of the 225 amino acid sequence of hCoV-NL63 ORF 3 predicted a triple membrane-spanning protein. Expression in infected CaCo-2 and LLC-MK2 cells was confirmed by immunofluorescence and Western blot analysis. The protein was detected within the endoplasmatic reticulum/Golgi intermediate compartment (ERGIC) where coronavirus assembly and budding takes place. Subcellular localization studies using recombinant ORF 3 protein transfected in Huh-7 cells revealed occurrence in ERGIC, Golgi- and lysosomal compartments. By fluorescence microscopy of differently tagged envelope (E), membrane (M) and nucleocapsid (N) proteins it was shown that ORF 3 protein colocalizes extensively with E and M within the ERGIC. Using N-terminally FLAG-tagged ORF 3 protein and an antiserum specific to the C-terminus we verified the proposed topology of an extracellular N-terminus and a cytosolic C-terminus. By in-vitro translation analysis and subsequent endoglycosidase H digestion we showed that ORF 3 protein is N-glycosylated at the N-terminus. Analysis of purified viral particles revealed that ORF 3 protein is incorporated into virions and is therefore an additional structural protein. Conclusions: This study is the first extensive expression analysis of a group 1 hCoV-ORF 3 protein. We give evidence that ORF 3 protein is a structural N-glycosylated and virion-incorporated protein.
PLANT HORMONE PATHWAYS PLAY A CRUCIAL ROLE IN SOLANUM SPP. INTERACTIONS WITH THE SOIL ENVIRONMENT
Creators: name: French, Elizabeth A.
Publisher: name: Purdue University Graduate School
Year: 2019
Subjects: Horticultural crop protection (incl. pests, diseases and weeds)
Plant biology not elsewhere classified
Microbial ecology
Plant pathology

description: Plants regulate responses to their environment through complex hormone signaling; these hormones can be categorized broadly into two categories: growth and defense, though many have roles in both. Much remains to be understood about the complexity of hormone signaling in relation to environmental responses, especially species- and genotype-specific differences. Unraveling this complexity of hormone signaling will lead to the development of resilient crops that are able to respond appropriately to their environment. In this dissertation, I hypothesize novel roles for growth and defense hormones in Solanum spp. responses to 1) biochar, a black carbon soil amendment (Chapter 2), 2) infection with Ralstonia solanacearum, an economically important soilborne pathogen causing bacterial wilt (Chapter 3), and 3) endophytic colonization by the soil bacterial community (Chapter 4). In Chapter 2, I showed that biochar upregulates GA signaling and affects GA-related traits in a species- and cultivar-specific manner. Biochar amendment also downregulates defense signaling. In Chapter 3, I demonstrated a novel role for auxin in resistance against R. solanacearum, including differential expression of auxin signaling genes in resistant genotype H7996 compared to susceptible WV in response to R. solanacearum infection. In addition, I observed stronger and faster upregulation of defense hormone marker genes for SA and ET in H7996 compared to WV. In Chapter 4, I showed that SA and ET are required for normal tomato root microbial community assembly, affecting the colonization of a few key taxa in order to promote alpha diversity. H7996 and WV root communities differ in alpha diversity, and a panel of H7996 x WV RILs showed quantitative variation in alpha diversity that correlated negatively with the abundance of these key taxa. In conclusion, I elucidated novel roles for hormones in responses to the soil environment, pathogen infection, and root community colonization. These findings are important for developing resilient, sustainable crops.
Additional file 2 of Investigating the effect of a school-based WASH intervention on soil-transmitted helminth and schistosome infections and nutritional status of school children in Ethiopia: a quasi-experimental study
Creators: name: Tadesse, Gemechu
name: Wuletaw, Yonas
name: Mekete, Kalkidan
name: Sime, Heven
name: Yard, Elodie
name: Appleby, Laura
name: Grimes, Jack
name: Dejene, Nigussie
name: Gardiner, Iain
name: Kazienga, Adama
name: Abbeddou, Souheila
name: French, Michael
name: Levecke, Bruno
name: Drake, Lesley
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Environmental Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Science Policy
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Computational Biology

description: Additional file 2: Table S2. The baseline response of the 24 schools to 17 questions about water, sanitation and hygiene.
MICROSCOPY IMAGE REGISTRATION, SYNTHESIS AND SEGMENTATION
Creators: name: Fu, Chichen
Publisher: name: Purdue University Graduate School
Year: 2019
Subjects: Electrical engineering not elsewhere classified
Digital processor architectures
Other information and computing sciences not elsewhere classified

description: Fluorescence microscopy has emerged as a powerful tool for studying cell biology because it enables the acquisition of 3D image volumes deeper into tissue and the imaging of complex subcellular structures. Fluorescence microscopy images are frequently distorted by motion resulting from animal respiration and heartbeat which complicates the quantitative analysis of biological structures needed to characterize the structure and constituency of tissue volumes. This thesis describes a two pronged approach to quantitative analysis consisting of non-rigid registration and deep convolutional neural network segmentation. The proposed image registration method is capable of correcting motion artifacts in three dimensional fluorescence microscopy images collected over time. In particular, our method uses 3D B-Spline based nonrigid registration using a coarse-to-fine strategy to register stacks of images collected at different time intervals and 4D rigid registration to register 3D volumes over time. The results show that the proposed method has the ability of correcting global motion artifacts of sample tissues in four dimensional space, thereby revealing the motility of individual cells in the tissue.
We describe in thesis nuclei segmentation methods using deep convolutional neural networks, data augmentation to generate training images of different shapes and contrasts, a refinement process combining segmentation results of horizontal, frontal, and sagittal planes in a volume, and a watershed technique to enumerate the nuclei. Our results indicate that compared to 3D ground truth data, our method can successfully segment and count 3D nuclei. Furthermore, a microscopy image synthesis method based on spatially constrained cycle-consistent adversarial networks is used to efficiently generate training data. A 3D modified U-Net network is trained with a combination of Dice loss and binary cross entropy metrics to achieve accurate nuclei segmentation. A multi-task U-Net is utilized to resolve overlapping nuclei. This method was found to achieve high accuracy object-based and voxel-based evaluations.
title: Farm adaptation to stricter nutrient management legislation and the implications for future livestock production: a review
Creators: name: Méité, René
name: Artner-Nehls, Astrid
name: Uthes, Sandra
Publisher: name: Humboldt-Universität zu Berlin
Year: 2024
Subjects: subject: Manure-based innovation
subject: Environmental impacts
subject: Nitrogen surplus
subject: Reduction potential
subject: Circularity
subject: Bioeconomy
subject: 570 Biologie

description: Stricter nutrient surplus and lower emission targets are placing adaptation pressure on livestock farms in European countries, while the public debate on how farms should react upon this pressure is controversial. In this paper, we performed an integrative literature review on different farm adaptation options, including (i) structural adaptation, (ii) technological adaptation, (iii) manure redistribution, and (iv) farm system conversion. Our analysis showed that stricter environmental legislation, together with complementary supply- and demand-related drivers, induced various farm responses. These ranged from easy to implement solutions (e.g. moderate livestock destocking to the legal levels combined with the adoption of emission abatement technologies) to novel niche concepts (e.g. animal husbandry without slaughtering). Possible reactions of farmers differed in their complexity and feasibility across different farm types and involved different trade-offs between environmental and socioeconomic interests. Future livestock production is expected to be diverse with different coexisting models that combine features of different adaptation options. The private and public sustainability of these models depends on their ability to simultaneously address multiple emerging challenges (e.g. emission reduction, animal welfare, biosecurity, and circularity). Current technological solutions often focus on a single objective (e.g. emission reduction). Integrated and multifunctional solutions (e.g. low-emission animal welfare barns compatible with biosecurity measures) are not yet sufficiently adopted, which calls for a rethinking and shifting in priorities in legislation and funding schemes.