Additional file 1 of Vertically stratified methane, nitrogen and sulphur cycling and coupling mechanisms in mangrove sediment microbiomes
Creators: name: Qian, Lu
name: Yu, Xiaoli
name: Gu, Hang
name: Liu, Fei
name: Fan, Yijun
name: Wang, Cheng
name: He, Qiang
name: Tian, Yun
name: Peng, Yisheng
name: Shu, Longfei
name: Wang, Shanquan
name: Huang, Zhijian
name: Yan, Qingyun
name: He, Jianguo
name: Liu, Guangli
name: Tu, Qichao
name: He, Zhili
Publisher: name: figshare
Year: 2024
Subjects: subject: Microbiology
subject: FOS: Biological sciences
subject: Geochemistry
subject: FOS: Earth and related environmental sciences

description: Additional file 1: Table S1. Summary of sequence information of different samples in each step of metagenome sequencing analysis. Table S2. Multiple response permutation procedure (MRPP) analysis of functional profiles related to methane, nitrogen and sulphur cycling among different depths. Table S3. Analysis of similarity (ANOSIM) based on functional profiles related to methane, nitrogen and sulphur cycling among different depths. Table S4. The vertical distribution of physicochemical characteristics of all mangrove sediment samples in this study. Table S5. Summary statistics for Mantel tests of correlations between metabolic pathways and environmental factors. Table S6. Summary of correlations between functional groups and keystones. Table S7. Summary of correlations between functional groups in the deep sediment. Fig. S1. The location of the sampling site at the Qi’ao Mangrove Reserve, Zhuhai, Guangdong province, China. Fig. S2. The depth-dependent profile of community structure in mangrove sediments (a) and linear regression analysis of correlations between the relative abundance of Proteobacteria and Euryarchaeota (b). Fig. S3. Principal coordinate analysis (PCoA) plot of all microbial functions (a), methane cycling (b), nitrogen cycling (c), and sulphur cycling (d). Fig. S4. Principal coordinate analysis (PCoA) plot of all microbial communities (a), and CH4-cycling (b), N-cycling (c) and S-cycling (d) microbial communities. Fig. S5. A heatmap plot of functional pathways for predicted open reading frames (ORFs) from metagenome sequence reads for sediment samples at different depths. Fig. S6. The vertical distribution of relative abundances of key gene families involved in methane cycling. Fig. S7. The vertical distribution of relative abundances of key gene families involved in nitrogen cycling. Fig. S8. The vertical distribution of the relative abundance of key microbial taxa (top 5) responsible for genes involved in S oxidation and denitrification. Fig. S9. The vertical distribution of relative abundances of key gene families involved in sulphur cycling. Fig. S10. Correlations between the relative abundance of key gene families. Fig. S11. Methane, nitrogen and sulphur cycling co-occurrence networks of three sediment layers at the species level with OTUs colored by phylum/class. Fig. S12. The vertical distribution of relative abundances of retrieved methanogen/ANME/SRB MAGs. Fig. S13. Metabolic profiles of retrieved MAGs (A) and relative abundances of selected MAGs (B). Fig. S14. Carbon, nitrogen, sulphur and metal metabolisms involved in different selected lineages.