Additional file 1 of Sex-dependent effects of ambient PM2.5 pollution on insulin sensitivity and hepatic lipid metabolism in mice
Creators: name: Li, Ran
name: Sun, Qing
name: Lam, Sin Man
name: Chen, Rucheng
name: Zhu, Junyao
name: Gu, Weijia
name: Zhang, Lu
name: Tian, He
name: Zhang, Kezhong
name: Chen, Lung-Chi
name: Sun, Qinghua
name: Shui, Guanghou
name: Liu, Cuiqing
Publisher: name: figshare
Year: 2020
Subjects: subject: Biochemistry
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Physiology
subject: Pharmacology
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Cancer
subject: Computational Biology

description: Additional file 1: Supplemental Materials. Supplemental Figure 1. Body weight and fasting blood glucose before PM2.5 exposure in C57BL/6 mice. A, Absolute value of body weight. B, Absolute value of fasting blood glucose. C, Fold change of body weight relative to FA. D, Fold change of blood glucose relative to FA. n=8. Supplemental Figure 2. Effects of PM2.5 exposure on body weight and glucose homeostasis in C57BL/6 mice at the end of PM2.5 exposure. A, Fold change of body weight relative to FA. B, Fold change of fasting blood glucose relative to FA. C-D, Fold change of fasting insulin levels (C) and HOMA-IR (D). E, Fold change of AUC (area under curves) of ITT relative to FA. *P&lt;0.05, **P&lt;0.01 for each comparison. n=5-8. Supplemental Figure 3. Effects of PM2.5 exposure on neutral lipids and cholesterol ester (CE) in the liver. A, Fold change of total DAG levels relative to FA. B, Fold change of total TAG levels relative to FA. C, Fold change of total cholesteroal levels relative to FA. D, Levels of total CE levels. E-F, levels in CE species in male (E) and female (F) mice. G, Fold change of total CE levels relative to FA. H-I, Fold change of levels in CE species relative to FA in male (H) and female (I) mice.*P&lt;0.05 for each comparison. n=5. Supplemental Figure 4. Effects of PM2.5 exposure on FFA profile and relevant signals in the liver. A, Fold change of total FFA content relative to FA in the hepatic lipid extracts from mice. B and C, Fold change of major species of FFA relative to FA in the hepatic lipid extracts from male (B) and female (C) mice. D and E, Fold change of analyzed protein levels of ApoB, MTTP, ATGL and HSL relative to FA in liver tissue from male (D) and female (E) mice. *P&lt;0.05 for each comparison. n=5 for lipidomic analysis and n=6 for protein examination. Supplemental Figure 5. Effects of PM2.5 exposure on plasmalogen levels and relevant signals in the liver. A, Fold change of total plasmalogen content relative to FA in the hepatic lipid extracts in mice. B, Fold change of major plasmalogen species relative to FA in the hepatic lipid extracts of female mice. C and D, Fold change of analyzed protein levels of CPT1αand ACOX1 relative to FA in the liver of male (C) and female (D) mice. *P&lt;0.05, **P&lt;0.01 for each comparison. n=5 for lipidomic analysis and n=6 for protein examination. Supplemental Figure 6. Effects of PM2.5 exposure on corticosteroids and sex hormones in mice. A and B, Fold change in levels of corticosteroids relative to FA in plasma from male (A) and female (B) mice. C and D, Levels of sex hormones in plasma from male (C) and female (D) mice. E and F, Fold change in levels of sex hormones relative to FA in plasma from male (E) and female (F) mice. *P&lt;0.05 for each comparison. n=4. Supplemental Table 1. Elemental constituents of the exposed PM2.5.