Additional file 2 of Video augmentation of the WHO cone assay to quantify mosquito behavioural responses to insecticide-treated nets
Creators: name: Jones, Jeff
name: Matope, Agnes
name: Barreaux, Priscille
name: Gleave, Katherine
name: Steen, Keith
name: Ranson, Hilary
name: McCall, Philip J.
name: Foster, Geraldine M.
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Neuroscience
subject: Biotechnology
subject: Cancer
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Computational Biology

description: Additional file 2: Figure S2. Treatment effect on longevity as the wing length measurement increases by An. gambiae strain based on the fitted mixed-effects Cox regression model. a Effect by treatment, b contrast (insecticide-treated net [ITN] vs untreated netting) effect, KS Kisumu, NG N’Gousso, BF Banfora, UT untreated net, IG2 Interceptor® G2 net, OS Olyset net, P2 PermaNet 2.0 net, and the solid black horizontal line is the reference (panel b, HR 1) with values above 1 showing more likelihood of dying when exposed to an ITN compared to UT and vice versa. Figure S3. Treatment effect on longevity as the activity at the lower half of the cone increases based on the fitted mixed-effects Cox regression model (VK7 strain only). a Effect by treatment, b contrast (insecticide-treated net [ITN] vs untreated netting) effect, UT untreated net, IG2 Interceptor® G2 net, OS Olyset net, P2 PermaNet 2.0 net, and the solid black horizontal line is the reference (panel b, HR 1) with values above one showing more likelihood of dying when exposed to an ITN compared to UT and vice versa. Table S1. Total movement activity, insecticide treated net versus untreated netting comparisons within An. gambiae strain from a linear regression model. Multiple pairwise comparisons 95% confidence intervals and P-values corrected using the Dunnett adjustment. UT untreated net, IG2 Interceptor® G2 net, OS Olyset net, P2 PermaNet 2.0 net, P3 PermaNet 3.0 net, KS Kisumu, NG N’Gousso and BF Banfora. Table S2. Treatment comparisons for blood-feeding success post-exposure based on generalised linear mixed models (GLMMs) with a binomial distribution fitted for each An. gambiae strain separately within the time the blood meal was offered (1 or 24 h). Only the predictor variables in the final model for each strain are provided. KS Kisumu, NG N’Gousso, BF Banfora, UT untreated net, IG2 Interceptor® G2 net, P2 PermaNet 2.0 net, OS Olyset net, Ref reference group, OR odds ratio, CI confidence interval, *significant at 5% significance level. Random effects include testing day and replicate. Table S3. Blood meal size results based on linear mixed effects models fitted for each An. gambiae strain separately. Only the predictor variables in the final model for each strain are provided. KS Kisumu, NG N’Gousso, BF Banfora, UT untreated net, IG2 Interceptor® G2 net, P2 PermaNet 2.0 net, OS Olyset net, Ref reference group, CI confidence interval, SD standard deviation, cone activity = predicted proportion of activity at the lower half of the cone during exposure (behaviour data), DE during exposure, *significant at 5% significance level. Random effects include testing day and replicate. Table S4. Mortality at 24-h results based on generalised linear mixed models (GLMMs) with a binomial distribution fitted for each An. gambiae strain separately within the time the blood meal was offered (1 or 24 h). Only the predictor variables in the final model for each strain are provided. KS Kisumu, NG N’Gousso, BF Banfora, UT untreated net, IG2 Interceptor® G2 net, P2 PermaNet 2.0 net, OS Olyset net, Ref reference group, OR odds ratio, CI confidence interval, *significant at 5% significance level. Random effects include testing day and replicate. Table S5. Longevity results based on the final model mixed-effects Cox regression models fitted for each An. gambiae strain separately. Only the predictor variables in the final model for each strain are provided. KS Kisumu, NG N’Gousso, BF Banfora, UT untreated net, IG2 Interceptor® G2 net, P2 PermaNet 2.0 net, OS Olyset net, Ref reference group, CI confidence interval, SD standard deviation, min minimum, max maximum, HR hazard ratio, cone activity = predicted proportion of activity at the lower half of the cone during exposure (behaviour data), DE during exposure, *significant at 5% significance level and +overall descriptive results. Random effects include testing day and replicate.