Bradyrhizobium oropedii Avontuur &amp; Palmer &amp; Beukes &amp; Chan &amp; Tasiya &amp; Zyl &amp; Coetzee &amp; Stepkowski &amp; Venter &amp; Steenkamp 2022, sp. nov.
Creators: name: Avontuur, Juanita R.
name: Palmer, Marike
name: Beukes, Chrizelle W.
name: Chan, Wai Y.
name: Tasiya, Taponeswa
name: Zyl, Elritha van
name: Coetzee, Martin P. A.
name: Stepkowski, Tomasz
name: Venter, Stephanus N.
name: Steenkamp, Emma T.
Publisher: name: Zenodo
Year: 2021
Subjects: Biodiversity
Taxonomy
Bacteria
Proteobacteria
Alphaproteobacteria
Rhizobiales
Bradyrhizobiaceae
Bradyrhizobium
Bradyrhizobium oropedii

description: <i>4.2. Description of Bradyrhizobium oropedii sp. nov.</i> <i>Bradyrhizobium oropedii</i> (o.ro.pe’ di.i. Gr. neut. n. <i>oropedion</i> mountain plain; N.L. gen. n. <i>oropedii</i>, of the central Southern African plateau, from where the species was isolated). Cells are motile, Gram-negative, rod shaped and approximately 1.87 × 0.48 μm in size. Growth occurs on YMA at 28 ◦ C after 7 days and colonies are translucent to opaque, circular, convex and cream in colour. Grows at pH values ranging from 5 to 10 and at temperatures ranging from 15 to 35 ◦ C. Isolates are unable to grow in the presence of more than 0.5% NaCl (w/v). Strains tested positive for oxidase, catalase, urease and β- glucosidase activity as well as the production of Tween 40 and Tween 80. Tested positive for the following carbon sources: Dfructose, L-fructose, D-gluconic acid, D-mannitol, α and β- hydroxy butyric acid, p-hydroxy phenylacetic acid, L-alaninamide, L-lactic acid, L-leucine, D-saccharic acid, and urocanic acid. Negative reactions were recorded for indole production and the assimilation of capric acid, adipic acid, malic acid, glycogen, α- D-lactose, N-acetyl-D-galactosamine, α- cyclodextrin, Adonitol, D-cellobiose, D-raffinose, i-erythritol, maltose, lactulose, D-melibiose, sucrose, D-trehalose, turanose, xylitol, L-threonine, uridine, inosine, thymine, glucose-1-phosphate and glucose-6-phosphate, and m-inositol. Resistant to ampicillin (10 μg/ml), chloramphenicol (30 μg/ml), tetracycline (30 μg/ml), penicillin (10 μg/ ml), and susceptible to streptomycin (25 μg/ml). The type strain, Pear76 T (=SARCC 731 = LMG 31408) was isolated from root nodules of <i>Pearsonia obovata</i> in the Dullstroom area of the Mpumalanga Province, South Africa and forms effective nodules on cowpea (<i>Vigna unguiculata</i>) and siratro (<i>Macroptilium atropurpureum</i>). The DNA G + Ccontent of the type strain is 63.5 mol%. GenBank/ EMBL/DDBJ accession numbers for the genome sequence and the gene sequences of this type strain are: whole genome (JACMYO000000000) and 16S rRNA (LR877297), <i>atpD</i> (LN650103), <i>glnII</i> (LN650145), <i>dnaK</i> (LN650124), <i>gyrB</i> (LN650208), <i>rpoB</i> (LN650187) and <i>nodA</i> (LN650250).
description: Published as part of <i>Avontuur, Juanita R., Palmer, Marike, Beukes, Chrizelle W., Chan, Wai Y., Tasiya, Taponeswa, Zyl, Elritha van, Coetzee, Martin P. A., Stepkowski, Tomasz, Venter, Stephanus N. &amp; Steenkamp, Emma T., 2022, Bradyrhizobium altum sp. nov., Bradyrhizobium oropedii sp. nov. and Bradyrhizobium acaciae sp. nov. from South Africa show locally restricted and pantropical nodA phylogeographic patterns, pp. 1-13 in Molecular Phylogenetics and Evolution (107338) (107338) 167</i> on page 10, DOI: 10.1016/j.ympev.2021.107338, http://zenodo.org/record/5728354