Additional file 1 of Refining and illuminating acetogenic Eubacterium strains for reclassification and metabolic engineering
Creators: name: Flaiz, Maximilian
name: Poehlein, Anja
name: Wilhelm, Wiebke
name: Mook, Alexander
name: Daniel, Rolf
name: Dürre, Peter
name: Bengelsdorf, Frank R.
Publisher: name: figshare
Year: 2024
Subjects: subject: Biochemistry
subject: Space Science
subject: Microbiology
subject: FOS: Biological sciences
subject: Genetics
subject: Evolutionary Biology
subject: Environmental Sciences not elsewhere classified
subject: Ecology
subject: Marine Biology

description: Additional file 1: Figure S1. Whole genome comparison of E. callanderi DSM 2593 with closely related Eubacterium strains. The reference genome and its size is indicated by the inner circle. The second and third circle represent the GC skew and GC content, respectively. E. callanderi strains are displayed in green, E. maltosivorans strains in purple, and E. limosum strains in red nuances. Orthologous genes are indicated with high, medium, and low identity showcased by respective color gradient in the figure legend. Phage regions (orange) and GIs (grey) are displayed on the outer circles. Figure S2. Whole genome comparison of E. callanderi DSM 2594 with closely related Eubacterium strains. The reference genome and its size is indicated by the inner circle. The second and third circle represent the GC skew and GC content, respectively. E. callanderi strains are displayed in green, E. maltosivorans strains in purple, and E. limosum strains in red nuances. Orthologous genes are indicated with high, medium, and low identity showcased by respective color gradient in the figure legend. Phage regions (orange) and GIs (grey) are displayed on the outer circles. Figure S3. Whole genome comparison of E. callanderi DSM 3468 with closely related Eubacterium strains. The reference genome and its size is indicated by the inner circle. The second and third circle represent the GC skew and GC content, respectively. E. callanderi strains are displayed in green, E. maltosivorans strains in purple, and E. limosum strains in red nuances. Orthologous genes are indicated with high, medium, and low identity showcased by respective color gradient in the figure legend. Phage regions (orange) and GIs (grey) are displayed on the outer circles. Figure S4. Whole genome comparison of E. callanderi DSM 107592 with closely related Eubacterium strains. The reference genome and its size is indicated by the inner circle. The second and third circle represent the GC skew and GC content, respectively. E. callanderi strains are displayed in green, E. maltosivorans strains in purple, and E. limosum strains in red nuances. Orthologous genes are indicated with high, medium, and low identity showcased by respective color gradient in the figure legend. Phage regions (orange) and GIs (grey) are displayed on the outer circles. Figure S5. Whole genome comparison of E. maltosivorans DSM 107592 with closely related Eubacterium strains. The reference genome and its size is indicated by the inner circle. The second and third circle represent the GC skew and GC content, respectively. E. maltosivorans strains are displayed in purple, E. callanderi strains in green, and E. limosum strains in red nuances. Orthologous genes are indicated with high, medium, and low identity showcased by respective color gradient in the figure legend. Phage regions (orange) and GIs (grey) are displayed on the outer circles. Figure S6. Average nucleotide identity (ANIm) analysis of eleven Eubacterium strains. Strains are separated into three distinct clades comprising E. limosum, E. callanderi, and E. maltosivorans. Figure S7. Arrangement of bcs/hcs operon genes of the eleven analyzed Eubacterium strains.