title: ÐÐ¸Ð½Ð°Ð¼Ð¸ÐºÐ° ÑÐµÐ¿ÑÐ¾Ð´ÑÐºÑÐ¸Ð¸ Ð²Ð¸ÑÑÑÐ° Ð³ÑÐ¸Ð¿Ð¿Ð° Ð¿ÑÐ¸Ñ Ð² ÐºÑÐ»ÑÑÑÑÐ°Ñ ÐºÐ»ÐµÑÐ¾Ðº Ð¸ Ð² ÑÐ°Ð·Ð²Ð¸Ð²Ð°ÑÑÐ¸Ñ ÑÑ ÐºÑÑÐ¸Ð½ÑÑ ÑÐ¼Ð±ÑÐ¸Ð¾Ð½Ð°Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Dynamics of avian influenza virus reproduction in cell cultures and in developing chicken embryos
Creators: name: Ð¢Ð°ÑÑÐ¾Ð²Ð°, ÐÐ»Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2025
Subjects: Ð³ÑÐ¸Ð¿Ð¿
Ð¸Ð·Ð¾Ð»ÑÑ Ð²Ð¸ÑÑÑÐ°
ÑÐ°Ð·Ð²Ð¸Ð²Ð°ÑÑÐ¸ÐµÑÑ ÐºÑÑÐ¸Ð½ÑÐµ ÑÐ¼Ð±ÑÐ¸Ð¾Ð½Ñ
ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐµ ÐºÑÐ»ÑÑÑÑÑ
Ð¿ÑÐ¸ÑÐ¸Ð¹ Ð³ÑÐ¸Ð¿Ð¿
Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐ° ÑÐµÐ¿ÑÐ¾Ð´ÑÐºÑÐ¸Ð¸
ÑÐµÐ°ÐºÑÐ¸Ñ Ð³ÐµÐ¼Ð°Ð³Ð³Ð»ÑÑÐ¸Ð½Ð°ÑÐ¸Ð¸
influenza
virus isolate
developing chicken embryos
cell culltures
avian influenza
reproduction dynamics
haemagglutination reaction

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð° Ð½Ð° ÑÑÐ°Ð²Ð½Ð¸ÑÐµÐ»ÑÐ½Ð¾Ðµ Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ ÑÐµÐ¿ÑÐ¾Ð´ÑÐºÑÐ¸Ð¸ Ð²Ð¸ÑÑÑÐ° Ð³ÑÐ¸Ð¿Ð¿Ð° Ð¿ÑÐ¸Ñ Ð² ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð»Ð¸Ð½Ð¸ÑÑ Ð¸ Ð² ÑÐ°Ð·Ð²Ð¸Ð²Ð°ÑÑÐ¸ÑÑÑ ÐºÑÑÐ¸Ð½ÑÑ ÑÐ¼Ð±ÑÐ¸Ð¾Ð½Ð°Ñ. ÐÐ±ÑÐµÐºÑÐ¾Ð¼ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐ²Ð»ÑÐµÑÑÑ Ð¸Ð·Ð¾Ð»ÑÑ Ð²Ð¸ÑÑÑÐ° Ð/ÑÑÐºÐ°/Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³/1123 (H1N1), Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½ÑÐ¹ Ð½Ð° ÑÐµÑÑÐ¸ÑÐ¾ÑÐ¸Ð¸ Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³Ð°. Ð ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð¿ÑÐµÐ´Ð¼ÐµÑÐ° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐ»ÑÐ¶Ð°Ñ ÑÐ°Ð·Ð²Ð¸Ð²Ð°ÑÑÐ¸ÐµÑÑ ÐºÑÑÐ¸Ð½ÑÐµ ÑÐ¼Ð±ÑÐ¸Ð¾Ð½Ñ Ð¸ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐµ Ð»Ð¸Ð½Ð¸Ð¸ ÑÐ°ÐºÐ¸Ðµ ÐºÐ°Ðº, MDCK, Vero, Ð549. Ð ÑÐ°Ð±Ð¾ÑÐµ Ð±ÑÐ»Ð¸ ÑÐµÑÐµÐ½Ñ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð·Ð°Ð´Ð°ÑÐ¸: 1. ÐÐ·ÑÑÐµÐ½Ñ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° ÑÑÐ°Ð¼Ð¼Ð° Ð²Ð¸ÑÑÑÐ° Ð³ÑÐ¸Ð¿Ð¿Ð°, Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¸Ð· Ð¾Ð±ÑÐ°Ð·ÑÐ¾Ð² Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ð°, Ð²Ð·ÑÑÐ¾Ð³Ð¾ Ð¾Ñ Ð´Ð¸ÐºÐ¸Ñ Ð¿ÑÐ¸Ñ, Ð² ÑÐ°Ð·Ð²Ð¸Ð²Ð°ÑÑÐ¸ÑÑÑ ÐºÑÑÐ¸Ð½ÑÑ ÑÐ¼Ð±ÑÐ¸Ð¾Ð½Ð°Ñ. 2. ÐÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð¾ÑÐµÐ½ÐºÐ° Ð¼Ð°ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸ÑÐµÑÐºÐ¸Ñ Ð¿Ð¾ÑÐ°Ð¶ÐµÐ½Ð¸Ð¹ ÑÐ°Ð·Ð²Ð¸Ð²Ð°ÑÑÐ¸ÑÑÑ ÐºÑÑÐ¸Ð½ÑÑ ÑÐ¼Ð±ÑÐ¸Ð¾Ð½Ð¾Ð², Ð·Ð°ÑÐ°Ð¶ÐµÐ½Ð½ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð¼ Ð/ÑÑÐºÐ°/Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³/1123 (H1N1). 3. ÐÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ðµ Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐ¸ ÑÐµÐ¿ÑÐ¾Ð´ÑÐºÑÐ¸Ð¸ ÑÑÐ°Ð¼Ð¼Ð° H1N1 Ð² ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð»Ð¸Ð½Ð¸ÑÑ MDCK, Vero, A549. ÐÑÐ¸ Ð½Ð°Ð¿Ð¸ÑÐ°Ð½Ð¸Ð¸ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±Ð·Ð¾ÑÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ: ÐÐ¸Ð±ÐµÑÐÐµÐ½Ð¸Ð½ÐºÐ°, eLIBRARY.RU, Scopus, ÐÐÐ ÐÐ¾Ð»Ð¸ÑÐµÑ. ÐÐ±ÑÐ°Ð±Ð¾ÑÐºÐ° Ð´Ð°Ð½Ð½ÑÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ° Ð±ÑÐ»Ð° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ñ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼: Microsoft Excel, Microsoft Word, GraphPad Prism 10.4.1. Ð ÑÐ°Ð±Ð¾ÑÐµ Ð±ÑÐ»Ð¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð¼ÐµÑÐ¾Ð´Ñ: ÑÐ¸ÑÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð²Ð¸ÑÑÑa Ð² ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ ÐºÑÐ»ÑÑÑÑÐ°Ñ Ð¸ Ð² ÑÐ°Ð·Ð²Ð¸Ð²Ð°ÑÑÐ¸ÑÑÑ ÐºÑÑÐ¸Ð½ÑÑ ÑÐ¼Ð±ÑÐ¸Ð¾Ð½Ð°Ñ, Ð¸ ÑÐµÐ°ÐºÑÐ¸Ñ Ð³ÐµÐ¼Ð°Ð³Ð³Ð»ÑÑÐ¸Ð½Ð°ÑÐ¸Ð¸, ÑÐ¸ÑÐ¾Ð¿Ð°ÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ Ð¼ÐµÑÐ¾Ð´Ñ, Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ. ÐÑÐµÐ´ÑÑÐ°Ð²Ð»ÐµÐ½Ð½ÑÐµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ñ Ð½Ð° Ð±Ð°Ð·Ðµ ÑÐµÐ½ÑÑÐ° Ð¤ÐÐÐ£ Â«ÐÐ¾ÑÑÐ´Ð°ÑÑÑÐ²ÐµÐ½Ð½ÑÐ¹ Ð½Ð°ÑÑÐ½Ð¾-Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÑÐºÐ¸Ð¹ Ð¸ÑÐ¿ÑÑÐ°ÑÐµÐ»ÑÐ½ÑÐ¹ Ð¸Ð½ÑÑÐ¸ÑÑÑ Ð²Ð¾ÐµÐ½Ð½Ð¾Ð¹ Ð¼ÐµÐ´Ð¸ÑÐ¸Ð½Ñ ÐÐ¸Ð½Ð¸ÑÑÐµÑÑÑÐ²Ð° Ð¾Ð±Ð¾ÑÐ¾Ð½Ñ Ð Ð¾ÑÑÐ¸Ð¹ÑÐºÐ¾Ð¹ Ð¤ÐµÐ´ÐµÑÐ°ÑÐ¸Ð¸Â». Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¸ Ð¸Ð·ÑÑÐµÐ½Ñ ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÐ¾ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾ÑÑÑ Ð¸ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ Ðº Ð³ÐµÐ¼Ð°Ð³Ð³Ð»ÑÑÐ¸Ð½Ð°ÑÐ¸Ð¸ Ð¸Ð·Ð¾Ð»ÑÑÐ° Ð²Ð¸ÑÑÑÐ° Ð³ÑÐ¸Ð¿Ð¿Ð° Ð¿ÑÐ¸Ñ Ð/ÑÑÐºÐ°/Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³/1123 (H1N1). ÐÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð¾ÑÐµÐ½ÐºÐ° Ð¼Ð°ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸ÑÐµÑÐºÐ¸Ñ Ð¿Ð¾ÑÐ°Ð¶ÐµÐ½Ð¸Ð¹ ÑÐ°Ð·Ð²Ð¸Ð²Ð°ÑÑÐ¸ÑÑÑ ÐºÑÑÐ¸Ð½ÑÑ ÑÐ¼Ð±ÑÐ¸Ð¾Ð½Ð¾Ð², Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð¿ÑÐ¸ ÑÐ°Ð·Ð¼Ð½Ð¾Ð¶ÐµÐ½Ð¸Ð¸ Ð²Ð¸ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¸Ð·Ð¾Ð»ÑÑÐ° Ð¿ÑÐ¸ ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÐµ 37ÂºÐ¡. ÐÑÐ» ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½ Ð²ÑÑÐ¾ÐºÐ¸Ð¹ ÑÑÐ¾Ð²ÐµÐ½Ñ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð¸ Ð¸Ð·Ð¾Ð»ÑÑÐ° Ð²Ð¸ÑÑÑÐ° Ð³ÑÐ¸Ð¿Ð¿Ð° Ð¿ÑÐ¸Ñ Ð/ÑÑÐºÐ°/Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³/1123 (H1N1) Ð² ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð»Ð¸Ð½Ð¸ÑÑ MDCK, Vero Ð¸ A549. ÐÐ° Ð¾ÑÐ½Ð¾Ð²Ð°Ð½Ð¸Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð² Ð¿Ð¾Ð´ÑÐ²ÐµÑÐ¶Ð´ÐµÐ½Ð° Ð²ÑÑÐ¾ÐºÐ°Ñ Ð°Ð´Ð°Ð¿ÑÐ¸Ð²Ð½Ð°Ñ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ Ð²Ð¸ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¸Ð·Ð¾Ð»ÑÑÐ° Ð/ÑÑÐºÐ°/Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³/1123 (H1N1) Ð² Ð¿Ð¾Ð¿ÑÐ»ÑÑÐ¸ÑÑ Ð¼Ð»ÐµÐºÐ¾Ð¿Ð¸ÑÐ°ÑÑÐ¸Ñ.
description: This work is aimed at comparative study of avian influenza virus reproduction in different cell lines and in developing chicken embryos. The object of the study is the isolate of the virus A/duck/St. Petersburg/1123 (H1N1) isolated in the territory of St. Petersburg. The subject of the study is developing chicken embryos and cell lines such as, MDCK, Vero, A549. The following tasks were solved in the work: 1. Biological characteristics of the influenza virus strain isolated from samples of material taken from wild birds in developing chicken embryos were stud-ied. 2. Macroscopic damage of developing chicken embryos infected with the strain A/ duck/St. Petersburg/1123 (H1N1) was evaluated. 3. The dynamics of reproduction of strain H1N1 in MDCK, Vero, A549 cell lines was compared. During the writing of the literature review were used databases such as: CyberLeninck, eLIBRARY.RU, Scopus, IBC Polytechnic. Experimental data processing was carried out using the following pro-grammes: Microsoft Excel, Microsoft Word, GraphPad Prism 10.4.1. The following methods were used in the work: virus titration in cell cultures and in developing chicken embryos, and hemagglutination reaction, cytopathological methods, microscopy. The presented studies were conducted at the State Institute for Experimental Military Medicine of the Ministry of Defense of the Russian Federation. The temperature resistance and hemagglutination ability of the isolate of avian influenza A/duck/St. Petersburg/1123 (H1N1) virus were studied. Macroscopic lesions of developing chicken embryos obtained when the virus isolate was propagated at 37ÂºC were evaluated. A high level of re-replication of the avi-an influenza A/duck/St. Petersburg/1123 (H1N1) virus isolate in MDCK, Vero and A549 cell lines was established. On the basis of the results obtained, a high adaptive capacity of the virus isolate A/duck/St. Petersburg/1123 (H1N1) in mammalian populations was confirmed.Â