title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ ÑÐ¿Ð¸ÑÐµÐ»Ð¸Ð°Ð»ÑÐ½Ð¾-Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð¿ÐµÑÐµÑ Ð¾Ð´Ð° Ð½Ð° ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð¼Ð°ÑÐºÐµÑÐ¾Ð² ÑÑÐ²Ð¾Ð»Ð¾Ð²Ð¾ÑÑÐ¸ Ð² ÐºÐ»ÐµÑÐºÐ°Ñ ÐºÐ¾Ð»Ð¾ÑÐµÐºÑÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÐ°ÐºÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: The influence of the induced epithelial-mesenchymal transition on the expression of stemness markers in colorectal cancer cells
Creators: name: Ð¢ÑÑÐºÐµÐµÐ²Ð°, ÐÐºÐ°ÑÐµÑÐ¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: ÑÐ¿Ð¸ÑÐµÐ»Ð¸Ð°Ð»ÑÐ½Ð¾-Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð°Ð»ÑÐ½ÑÐ¹ Ð¿ÐµÑÐµÑ Ð¾Ð´
Ð¾Ð¿ÑÑ Ð¾Ð»ÐµÐ²ÑÐµ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸
ÐºÐ¾Ð»Ð¾ÑÐµÐºÑÐ°Ð»ÑÐ½ÑÐ¹ ÑÐ°Ðº
ÐºÐ°Ð½ÑÐµÑÐ¾Ð³ÐµÐ½ÐµÐ·
ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸Ð¾Ð½Ð½ÑÐµ ÑÐ°ÐºÑÐ¾ÑÑ
epithelial-mesenchymal transition
cancer stem cells
colorectal cancer
tumorigenesis
transcription factors

description: ÐÐµÑÐµÑÐ¾Ð´ ÐºÐ°ÑÑÐ¸Ð½Ð¾Ð¼ Ð² ÑÑÐ°Ð´Ð¸Ñ Ð¼ÐµÑÐ°ÑÑÐ°Ð·Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¼Ð¾Ð¶ÐµÑ Ð±ÑÑÑ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÐµÐ½ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ñ ÑÐ¿Ð¸ÑÐµÐ»Ð¸Ð°Ð»ÑÐ½Ð¾-Ð¼ÐµÐ·ÐµÐ½ÑÐ¸Ð¼Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð¿ÐµÑÐµÑÐ¾Ð´Ð° (Ð­ÐÐ). Ð Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ Ð±ÑÐ»Ð¾ Ð¸Ð·ÑÑÐµÐ½Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð­ÐÐ Ð½Ð° ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ¸ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ Ð°Ð³ÑÐµÑÑÐ¸Ð²Ð½Ð¾Ð¹ Ð¿Ð¾Ð¿ÑÐ»ÑÑÐ¸Ð¸ Ð¾Ð¿ÑÑÐ¾Ð»ÐµÐ²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº â Ð¾Ð¿ÑÑÐ¾Ð»ÐµÐ²ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº (ÐÐ¡Ð). ÐÐ¾ÑÑÐµÐ´ÑÑÐ²Ð¾Ð¼ Ð²Ð²ÐµÐ´ÐµÐ½Ð¸Ñ Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð´Ð½Ð¾Ð¹ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ð¸, ÑÐ¾Ð´ÐµÑÐ¶Ð°ÑÐµÐ¹ ÐºÐ»Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¹ ZEB1, Ð±ÑÐ» Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½ Ð­ÐÐ. ÐÑÐ±Ð¾Ñ ÑÑÐ²Ð¾Ð»Ð¾Ð²Ð¾Ð³Ð¾ ÐºÐ¾Ð¼Ð¿Ð¾Ð½ÐµÐ½ÑÐ° Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»ÑÑ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÑÐµÐ¿Ð¾ÑÑÐµÑÐ½Ð¾Ð¹ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ð¸, ÑÐ¾Ð´ÐµÑÐ¶Ð°ÑÐµÐ¹ ÑÐ°Ð¹ÑÑ ÑÐ²ÑÐ·ÑÐ²Ð°Ð½Ð¸Ñ OCT4/SOX2. ÐÐµÑÐ¾Ð´Ð¾Ð¼ ÐÐ¢-ÐÐ¦Ð  Ð² ÑÐµÐ°Ð»ÑÐ½Ð¾Ð¼ Ð²ÑÐµÐ¼ÐµÐ½Ð¸ Ð±ÑÐ»Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ñ ÑÑÐ¾Ð²Ð½Ð¸ Ð¼Ð ÐÐ Ð¾ÑÐ½Ð¾Ð²Ð½ÑÑ Ð¼Ð°ÑÐºÐµÑÐ¾Ð² ÐÐ¡Ð. Ð£ÑÐ¾Ð²Ð½Ð¸ Ð¼Ð ÐÐ Ð´Ð°Ð½Ð½ÑÑ Ð¼Ð°ÑÐºÐµÑÐ¾Ð² Ð² ÐºÐ»Ð¾Ð½Ðµ Ñ Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¼ Ð­ÐÐ Ð±ÑÐ»Ð¸ Ð² Ð½ÐµÑÐºÐ¾Ð»ÑÐºÐ¾ ÑÐ°Ð· Ð²ÑÑÐµ, ÑÐµÐ¼ Ð² Ð¾Ð±ÑÑÐ½Ð¾Ð¼ ÑÑÐ²Ð¾Ð»Ð¾Ð²Ð¾Ð¼ ÐºÐ¾Ð¼Ð¿Ð¾Ð½ÐµÐ½ÑÐµ. ÐÐµÑÐ¼Ð¾ÑÑÑ Ð½Ð° Ð¿ÑÐµÐ²ÑÑÐµÐ½Ð¸Ðµ ÑÑÐ¾Ð²Ð½ÐµÐ¹ Ð¼Ð ÐÐ ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸Ð¾Ð½Ð½ÑÑ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð² TWIST Ð¸ SNAIL, Ð¾ÑÐ²ÐµÑÐ°ÑÑÐ¸Ñ Ð·Ð° Ð°ÐºÑÐ¸Ð²Ð°ÑÐ¸Ñ Ð­ÐÐ, Ð² ÑÑÐ²Ð¾Ð»Ð¾Ð²Ð¾Ð¼ ÐºÐ¾Ð¼Ð¿Ð¾Ð½ÐµÐ½ÑÐµ ÐºÐ»ÐµÑÐ¾Ðº Ð½Ðµ Ð±ÑÐ»Ð¾ Ð²ÑÑÐ²Ð»ÐµÐ½Ð¾ Ð¾ÑÐ»Ð¸ÑÐ¸Ð¹ Ð² ÑÑÐ¾Ð²Ð½ÑÑ Ð¼Ð ÐÐ ZEB1, Ð²Ð¸Ð¼ÐµÐ½ÑÐ¸Ð½Ð° Ð¸ Ð-ÐºÐ°Ð´Ð³ÐµÑÐ¸Ð½Ð°, Ð¾ÑÐ½Ð¾Ð²Ð½ÑÑ Ð¼Ð°ÑÐºÐµÑÐ¾Ð² Ð­ÐÐ. Ð ÑÐ¾ Ð¶Ðµ Ð²ÑÐµÐ¼Ñ Ð²ÑÑÐ²Ð»ÐµÐ½Ñ Ð¾ÑÐ»Ð¸ÑÐ¸Ñ Ð² Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¸ Ð¼Ð¸Ð³ÑÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÐ¸ ÐºÐ»ÐµÑÐ¾Ðº Â«Ð¿Ð¾Ð´Ð¾Ð±Ð½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÐ¼Â». Ð¢Ð°ÐºÐ¸Ð¼ Ð¾Ð±ÑÐ°Ð·Ð¾Ð¼, Ð¼Ð¾Ð¶Ð½Ð¾ Ð·Ð°ÐºÐ»ÑÑÐ¸ÑÑ, ÑÑÐ¾ Ð²Ð²ÐµÐ´ÐµÐ½Ð¸Ðµ ZEB1 Ð² ÐºÐ»ÐµÑÐºÐ¸ ÐºÐ¾Ð»Ð¾ÑÐµÐºÑÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÐ°ÐºÐ° Ð¿ÑÐ¸Ð²ÐµÐ»Ð¾ Ðº Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ ÐºÐ»Ð¾Ð½Ð¾Ð², Ð½Ðµ ÑÐ¾Ð»ÑÐºÐ¾ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸Ð·ÑÑÑÐ¸ÑÑÑ Ð¿Ð¾Ð²ÑÑÐµÐ½Ð½ÑÐ¼Ð¸ ÑÑÐ¾Ð²Ð½ÑÐ¼Ð¸ Ð¼Ð°ÑÐºÐµÑÐ¾Ð² ÐÐ¡Ð, Ð½Ð¾ Ð¸ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½ÑÑ Ðº Ð¼Ð¸Ð³ÑÐ°ÑÐ¸Ð¸.
description: The epithelial-mesenchymal transition is the main program that is responsible for metastasis. The given work is devoted to the exploration of the induced epithelial-mesenchymal transition (EMT) on cancer stem-like cells (CSC) influence, which are the most aggressive members of the cancer cells population. We used a special plasmid, which contains the ZEB1 gene, to induce the EMT program in the colorectal cancer cell line MIP101. The population of cancer stem-like cells was selected using construction with six binding sites for OCT4/SOX2 transcription factors. We have used the real-time RT-PÐ¡R method to examine the main CSC markerâs mRNA levels. We noticed that mRNA levels of these markers in cancer stem-like cells with induced EMT was higher than in the control group of cancer stem-like cells. Also, we noticed that high mRNA levels of transcriptional factors such as TWIST and SNAIL, which are involved in EMT, had not affected the mRNA levels of ZEB1, vimentin and E-cadherin, which are the EMT markers. On the other hand, there were some differences in morphology and migration ability of cancer like-stem cells under EMT and control group. Thus, it can be concluded that ZEB1 transfection in colorectal cancer cells can lead to increased levels of CSCâs markers as well as high migration ability.