title: ÐÐ·ÑÑÐµÐ½Ð¸Ðµ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ð³ÐµÑÐ¾Ð¿ÑÐ¾ÑÐµÐºÑÐ¾ÑÐ¾Ð² Ð½Ð° Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð¿ÐµÑÐ²Ð¸ÑÐ½ÑÑ ÑÐ¸Ð±ÑÐ¾Ð±Ð»Ð°ÑÑÐ¾Ð² ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Study of the action of geroprotectors on the model of primary human fibroblasts
Creators: name: ÐÑÑÐ¾Ð², ÐÐ»Ð°Ð´Ð¸ÑÐ»Ð°Ð²
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: ÑÐ¸Ð½Ð´ÑÐ¾Ð¼ ÐÐ¾ÐºÐºÐµÐ¹Ð½Ð°
SA-Î²-Gal
SkQ1
ÐÐµÑÑÐ¾ÑÐ¼Ð¸Ð½
Ð³ÐµÑÐ¾Ð¿ÑÐ¾ÑÐµÐºÑÐ¾ÑÑ
Fiji
ÑÑÐ°ÑÐµÐ½Ð¸Ðµ
cockayne syndrome
Metformin
geroprotectors
aging

description: Ð Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¾ Ð²Ð¾Ð·Ð´ÐµÐ¹ÑÑÐ²Ð¸Ðµ Ð³ÐµÑÐ¾Ð¿ÑÐ¾ÑÐµÐºÑÐ¾ÑÐ¾Ð² SkQ1 Ð¸ ÐÐµÑÑÐ¾ÑÐ¼Ð¸Ð½ Ð½Ð° ÐºÑÐ»ÑÑÑÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐ¸Ð±ÑÐ¾Ð±Ð»Ð°ÑÑÐ¾Ð² Ð·Ð´Ð¾ÑÐ¾Ð²ÑÑ Ð´Ð¾Ð½Ð¾ÑÐ¾Ð² 25 Ð¸ 75 Ð»ÐµÑ, Ð° ÑÐ°ÐºÐ¶Ðµ Ð´Ð¾Ð½Ð¾ÑÐ¾Ð² Ñ ÑÐ¸Ð½Ð´ÑÐ¾Ð¼Ð¾Ð¼ ÐÐ¾ÐºÐºÐµÐ¹Ð½Ð° 7 Ð¸ 13 Ð»ÐµÑ. Ð¡ÑÐ°Ð²Ð½Ð¸ÑÐµÐ»ÑÐ½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð· Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»ÑÑ Ð¿ÑÐ¸ Ð¿Ð¾Ð¼Ð¾ÑÐ¸ ÑÑÑÐ¼ÐºÐ¸ Ð¼Ð¸ÑÐ¾ÑÑÐµÐºÐµÑÐ¾Ð² Ð¿Ð¾ÑÑÐµÐ´ÑÑÐ²Ð¾Ð¼ ÐºÐ¾Ð½ÑÐ¾ÐºÐ°Ð»ÑÐ½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸, Ð° ÑÐ°ÐºÐ¶Ðµ ÑÑÑÐ¼ÐºÐ¸ SA-Î²-Gal Ð¼ÐµÑÐ¾Ð´Ð°Ð¼Ð¸ Ð¾Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸. ÐÐ°Ð»ÑÐ½ÐµÐ¹ÑÐ¸Ð¹ Ð°Ð½Ð°Ð»Ð¸Ð· Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»ÑÑ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼ Fiji Ð¸ Excel. Ð Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ Ð¾Ð¿Ð¸ÑÐ°Ð½Ð¾ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ðµ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ° Ð² ÑÐµÑÐµÐ½Ð¸Ðµ 4Ñ Ð¿Ð°ÑÑÐ°Ð¶ÐµÐ¹ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ ÐºÑÐ»ÑÑÑÑ, Ñ ÑÐ´Ð²Ð¾ÐµÐ½Ð¸ÐµÐ¼ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð° ÑÐ»Ð°ÐºÐ¾Ð½Ð¾Ð² Ð½Ð° ÐºÐ°Ð¶Ð´Ð¾Ð¼ Ð¿Ð°ÑÑÐ°Ð¶Ðµ Ð¸ Ð²ÑÑÐµÐ²Ðµ Ð½Ð° Ð´Ð¾Ð¿Ð¾Ð»Ð½Ð¸ÑÐµÐ»ÑÐ½ÑÐµ ÑÐ°ÑÐºÐ¸ ÐÐµÑÑÐ¸, Ð½Ð° ÐºÐ°Ð¶Ð´Ð¾Ð¼ Ð¿Ð°ÑÑÐ°Ð¶Ðµ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´Ð¸Ð»Ð°ÑÑ ÑÑÑÐ¼ÐºÐ° ÐºÑÐ»ÑÑÑÑ, ÐºÑÐ»ÑÑÑÑÑ ÐºÐ»ÐµÑÐ¾Ðº, Ð½ÐµÐ¿Ð¾ÑÑÐµÐ´ÑÑÐ²ÐµÐ½Ð½Ð¾ ÑÑÐ°ÑÑÐ²Ð¾Ð²Ð°Ð²ÑÐ¸Ðµ Ð² ÑÑÑÐ¼ÐºÐµ, Ð° ÑÑÐ¾ Ð¾Ð´Ð¸Ð½ ÑÐ»Ð°ÐºÐ¾Ð½ Ð¸ Ð¾Ð´Ð½Ð° ÑÐ°ÑÐºÐ° ÐÐµÑÑÐ¸ Ð´Ð»Ñ ÐºÐ°Ð¶Ð´Ð¾Ð¹ Ð»Ð¸Ð½Ð¸Ð¸, Ð²ÑÐ²Ð¾Ð´Ð¸Ð»Ð¸ÑÑ Ð¸Ð· ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°. Ð¢Ð°ÐºÐ¸Ð¼ Ð¾Ð±ÑÐ°Ð·Ð¾Ð¼ ÑÐ¾ÑÑÐ°Ð½ÑÐ»Ð¾ÑÑ Ð¿Ð¾ÑÑÐ¾ÑÐ½Ð½Ð¾Ðµ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð¾ ÑÐ»Ð°ÐºÐ¾Ð½Ð¾Ð² Ñ ÐºÐ»ÐµÑÐºÐ°Ð¼Ð¸ Ð² ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐµ. ÐÐ¾ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ°Ð¼ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¾ ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¾, ÑÑÐ¾ Ð¾Ð±Ð° Ð²ÐµÑÐµÑÑÐ²Ð° ÑÐ²Ð»ÑÑÑÑÑ ÑÑÑÐµÐºÑÐ¸Ð²Ð½ÑÐ¼Ð¸ Ð³ÐµÑÐ¾Ð¿ÑÐ¾ÑÐµÐºÑÐ¾ÑÐ°Ð¼Ð¸ Ñ ÑÐ°Ð·Ð½Ð¾Ð¹ Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð½Ð¾ÑÑÑÑ, Ð¿Ð¾ÐºÐ°Ð·Ð°Ð²ÑÐ¸Ð¼Ð¸ ÑÐ²Ð¾Ñ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ðµ ÐºÐ°Ðº Ð½Ð° Â«Ð·Ð´Ð¾ÑÐ¾Ð²ÑÑÂ» ÐºÑÐ»ÑÑÑÑÐ°Ñ, ÑÐ°Ðº Ð¸ Ð½Ð° ÐºÑÐ»ÑÑÑÑÐ°Ñ Ð¾Ñ Ð±Ð¾Ð»ÑÐ½ÑÑ ÑÐ¸Ð½Ð´ÑÐ¾Ð¼Ð¾Ð¼ ÐÐ¾ÐºÐºÐµÐ¹Ð½Ð°.
description: In this paper was investigated the effect of SkQ1 and Metformin geroprotectors on fibroblast cell cultures of healthy donors aged 25 and 75 years, as well as donors with Cockayne syndrome aged 7 and 13 years. The comparative analysis was carried out using mitotrackers by confocal microscopy, as well as SA-Î²-Gal imaging using optical microscopy. Further analysis was performed using the Fiji and Excel programs. This paper describes the experiment during 4 passages of cell cultures, with doubling the number of vials on each passage and seeding on additional Petri dishes, each passage was photographed. Cell cultures directly involved in the survey, and this is one vial and one Petri dish for each line, were deduced from the experiment. Thus, a constant number of vials with cells in the experiment was preserved.