Serum concentrations of MCP-1 and IL-6 in combination predict the presence of coronary artery disease and mortality in subjects undergoing coronary angiography
Creators: name: Hanachi, Parichehr
name: Tajfard, Mohammad
name: Latiff L.A
name: Rahimi H.R
name: Moohebati, Mohsen
name: Hasanzadeh M
name: Emrani A.S
name: Esmaeily H
name: Taghipour A
name: Mirhafez S.R
name: Ferns G.A
name: Mardan-Nik M
name: Mohammadzadeh E
name: Avan A
name: Ghayour-Mobarhan, Majid
Publisher: name: figshare
Year: 2021
Subjects: subject: 110199 Medical Biochemistry and Metabolomics not elsewhere classified
subject: FOS: Clinical medicine
subject: FOS: Clinical medicine
subject: 100302 Bioprocessing, Bioproduction and Bioproducts
subject: FOS: Industrial biotechnology
subject: FOS: Industrial biotechnology
subject: 60199 Biochemistry and Cell Biology not elsewhere classified
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Cell Biology
subject: 60114 Systems Biology
subject: 60101 Analytical Biochemistry
subject: 111201 Cancer Cell Biology
subject: 100499 Medical Biotechnology not elsewhere classified
subject: FOS: Medical biotechnology
subject: FOS: Medical biotechnology
subject: Biochemistry
subject: Biotechnology
subject: Molecular Biology
subject: Biological Techniques
subject: 111602 Human Biophysics
subject: FOS: Basic medicine
subject: FOS: Basic medicine
subject: 100202 Biological Control
subject: FOS: Environmental biotechnology
subject: FOS: Environmental biotechnology
subject: 69999 Biological Sciences not elsewhere classified
subject: Health Care
subject: 119999 Medical and Health Sciences not elsewhere classified
subject: FOS: Other medical sciences
subject: FOS: Other medical sciences
subject: Diseases

description: AbstractCytokines play a key role in the pathogenesis of coronary artery disease (CAD). The aim of current study was to investigate the relationship between the serum concentrations of 12 cytokines with mortality and extent of CAD in individuals undergoing angiography and healthy controls. 342 CAD patients were recruited and divided into 2 groups: those with ≥50% occlusion in at least one coronary artery [Angiography (+)] or &lt;50% obstruction in coronary arteries [Angiography (−)]. Also 120 healthy subjects were enrolled as control group. Lipid profile, fasting blood glucose, body mass index, and blood pressure were evaluated in all the subjects. An Evidence Investigator® was used for measuring 12 cytokines (IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, TNF-α, MCP-1, IFN-γ, EGF, VEGF) using sandwich chemiluminescent assays. Univariate analysis, multivariate regression models, ROC, and Kaplan–Meier survival curves were used for exploring the candidate markers in CAD patients. Serum level of IFN-γ, IL-4, MCP-1, EGF, IL-6, and IL-8 were markedly higher in angiogram-positive patients, while VEGF concentrations were significantly (P &lt; 0.05) lower, compared to control group. ROC analysis for MCP-1 showed that a cut-off of 61.95 pg/mL had 91% sensitivity and 91% specificity for predicting CAD patients. Moreover, &gt;2.16 pg/mL IL-6 had a &gt; 94% sensitivity and 70% specificity in predicting 2 years mortality in the subjects with a serum MCP-1 &gt; 61.95 pg/ mL, and patients having IL-6/MCP-1 combination had a shorter survival.Our findings demonstrate that CAD patients with serum MCP-1 and IL-6 levels of &gt;61.95 and &gt;2.16 pg/mL had a higher mortality with 94.1% sensitivity and 70.5% specificity for predicting mortality in CAD patients.