title: ÐÑÐ¾Ð´ÑÐºÑÐ¸Ñ Ð±ÐµÐ»ÐºÐ¾Ð², ÑÑÐ°ÑÑÐ²ÑÑÑÐ¸Ñ Ð² ÑÐµÐ³ÑÐ»ÑÑÐ¸Ð¸ Ð°Ð¿Ð¾Ð¿ÑÐ¾Ð·Ð° Ð¸ ÑÐ¸ÑÐºÐ°Ð´Ð½ÑÑ ÑÐ¸ÑÐ¼Ð¾Ð², Ñ ÐºÑÑÑ Ð»Ð¸Ð½Ð¸Ð¸ ÐÑÑÑÐ¸Ð½ÑÐºÐ¾Ð³Ð¾-ÐÐ¾Ð»Ð¾Ð´ÐºÐ¸Ð½Ð¾Ð¹
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Production of proteins, which take part in the regulation of apoptosis and circadian rhythms in the Krushinsky-Molodkina rat line
Creators: name: ÐÑÐ¶Ð½Ð¾Ð²Ð°, ÐÐ»Ð¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Ð²Ð¸ÑÐ¾ÑÐ½Ð°Ñ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ñ
Ð°ÑÐ´Ð¸Ð¾Ð³ÐµÐ½Ð½ÑÐ¹ ÐºÐ¸Ð½Ð´Ð»Ð¸Ð½Ð³
ÐºÑÑÑÑ Ð»Ð¸Ð½Ð¸Ð¸ ÐÑÑÑÐ¸Ð½ÑÐºÐ¾Ð³Ð¾-ÐÐ¾Ð»Ð¾Ð´ÐºÐ¸Ð½Ð¾Ð¹
Ð°Ð¿Ð¾Ð¿ÑÐ¾Ð·Ð½ÑÐµ Ð±ÐµÐ»ÐºÐ¸
p53
Ð±ÐµÐ»ÐºÐ¸ ÑÐ¸ÑÐºÐ°Ð´Ð½Ð¾Ð³Ð¾ ÑÐ¸ÑÐ¼Ð°
CLOCK
ÑÐµÐ´Ð¾ÐºÑ-ÑÑÐ²ÑÑÐ²Ð¸ÑÐµÐ»ÑÐ½ÑÐµ Ð±ÐµÐ»ÐºÐ¸
Nrf2
temporal lobe epilepsy
audiogenic kindling
Krushinsky-Molodkina rat line
apoptotic proteins
circadian rhythm proteins
redox-sensitive proteins

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ ÑÑÐ¾Ð²Ð½ÐµÐ¹ Ð±ÐµÐ»ÐºÐ¾Ð², ÑÑÐ°ÑÑÐ²ÑÑÑÐ¸Ñ Ð² ÑÐµÐ³ÑÐ»ÑÑÐ¸Ð¸ Ð°Ð¿Ð¾Ð¿ÑÐ¾Ð·Ð°, ÑÐ¸ÐºÐ»Ð° ÑÐ½Ð°-Ð±Ð¾Ð´ÑÑÑÐ²Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¸ Ð·Ð°ÑÐ¸ÑÑ Ð¾Ñ Ð¾ÐºÐ¸ÑÐ»Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ ÑÑÑÐµÑÑÐ° Ð² ÐºÐ¾ÑÐµ Ð²Ð¸ÑÐ¾ÑÐ½Ð¾Ð¹ Ð´Ð¾Ð»Ð¸ Ð³Ð¾Ð»Ð¾Ð²Ð½Ð¾Ð³Ð¾ Ð¼Ð¾Ð·Ð³Ð° Ð¿ÑÐ¸ Ð°ÑÐ´Ð¸Ð¾Ð³ÐµÐ½Ð½Ð¾Ð¹ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ð¸ Ð½Ð° Ð¼Ð¾Ð´ÐµÐ»Ð¸ ÐºÑÑÑ Ð»Ð¸Ð½Ð¸Ð¸ ÐÑÑÑÐ¸Ð½ÑÐºÐ¾Ð³Ð¾-ÐÐ¾Ð»Ð¾Ð´ÐºÐ¸Ð½Ð¾Ð¹. ÐÐ»Ñ ÑÑÐ¾Ð³Ð¾ ÐºÑÑÑ Ð»Ð¸Ð½Ð¸Ð¸ ÐÑÑÑÐ¸Ð½ÑÐºÐ¾Ð³Ð¾-ÐÐ¾Ð»Ð¾Ð´ÐºÐ¸Ð½Ð¾Ð¹ (ÐÐ) Ð¿Ð¾Ð´Ð²ÐµÑÐ³Ð°Ð»Ð¸ Ð°ÑÐ´Ð¸Ð¾Ð³ÐµÐ½Ð½Ð¾Ð¼Ñ ÐºÐ¸Ð½Ð´Ð»Ð¸Ð½Ð³Ñ Ð² ÑÐµÑÐµÐ½Ð¸Ðµ 7 Ð¸ 21 Ð´Ð½ÐµÐ¹, Ð¿Ð¾ÑÐ»Ðµ ÐºÐ¾ÑÐ¾ÑÐ¾Ð³Ð¾ ÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð»Ð¸ 7 Ð´Ð½ÐµÐ¹ Ð¿Ð¾ÐºÐ¾Ñ. ÐÐ°ÑÐµÑÐ¸Ð°Ð» Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â ÐºÐ¾ÑÐ° Ð²Ð¸ÑÐ¾ÑÐ½Ð¾Ð¹ Ð´Ð¾Ð»Ð¸ Ð³Ð¾Ð»Ð¾Ð²Ð½Ð¾Ð³Ð¾ Ð¼Ð¾Ð·Ð³Ð°. ÐÐ° ÑÑÐµÐ·Ð°Ñ ÐºÐ¾ÑÑ Ð¼Ð¾Ð·Ð³Ð° Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»Ð¸ Ð¸Ð¼Ð¼ÑÐ½Ð¾Ð³Ð¸ÑÑÐ¾ÑÐ¸Ð¼Ð¸ÑÐµÑÐºÑÑ ÑÐµÐ°ÐºÑÐ¸Ñ Ñ Ð°Ð½ÑÐ¸ÑÐµÐ»Ð°Ð¼Ð¸ Ðº p53, CLOCK, Nrf2 Ð¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð»Ð¸ ÑÑÐ¾Ð²ÐµÐ½Ñ Ð°Ð¿Ð¾Ð¿ÑÐ¾Ð·Ð°. ÐÐ°Ð»ÐµÐµ ÑÑÐ°Ð²Ð½Ð¸Ð²Ð°Ð»Ð¸ Ð¾Ð¿ÑÐ¸ÑÐµÑÐºÑÑ Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÑ Ð¾ÐºÑÐ°ÑÐµÐ½Ð½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð¸ Ð°Ð±ÑÐ¾Ð»ÑÑÐ½Ð¾Ðµ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð¾ Ð°Ð¿Ð¾Ð¿ÑÐ¾Ð·Ð½ÑÑ ÑÐµÐ»ÐµÑ Ð² ÐºÐ¾ÑÐµ Ð²Ð¸ÑÐ¾ÑÐ½Ð¾Ð¹ Ð´Ð¾Ð»Ð¸ Ð¼Ð¾Ð·Ð³Ð° ÐºÑÑÑ Ð»Ð¸Ð½Ð¸Ð¸ ÐÑÑÑÐ¸Ð½ÑÐºÐ¾Ð³Ð¾-ÐÐ¾Ð»Ð¾Ð´ÐºÐ¸Ð½Ð¾Ð¹ Ñ Ð°Ð½Ð°Ð»Ð¾Ð³Ð¸ÑÐ½ÑÐ¼Ð¸ Ð¿Ð¾ÐºÐ°Ð·Ð°ÑÐµÐ»ÑÐ¼Ð¸ Ñ Ð¸Ð½ÑÐ°ÐºÑÐ½ÑÑ ÐºÑÑÑ ÑÐ¾Ð¹ Ð¶Ðµ Ð»Ð¸Ð½Ð¸Ð¸, Ð° ÑÐ°ÐºÐ¶Ðµ ÑÑÐ°Ð²Ð½Ð¸Ð²Ð°Ð»Ð¸ Ð¿Ð¾ÐºÐ°Ð·Ð°ÑÐµÐ»Ð¸ Ð¸Ð½ÑÐ°ÐºÑÐ½ÑÑ ÐºÑÑÑ ÐÐ Ð¸ ÐÐ¸ÑÑÐ°Ñ.Â  ÐÑ Ð¾Ð±Ð½Ð°ÑÑÐ¶Ð¸Ð»Ð¸, ÑÑÐ¾ ÑÑÐ¾Ð²ÐµÐ½Ñ p53 Ð¸ CLOCK Ð¿Ð¾Ð½Ð¸Ð¶ÐµÐ½ Ñ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½ÑÑ ÐºÑÑÑ ÐÐ Ð¿Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ Ñ ÐºÑÑÑÐ°Ð¼Ð¸ ÐÐ¸ÑÑÐ°Ñ, Ð° ÑÐ¸Ð½ÑÐµÐ· Nrf2 Ð¾ÑÑÐ°ÐµÑÑÑ Ð½ÐµÐ¸Ð·Ð¼ÐµÐ½Ð½ÑÐ¼. Ð£ÑÐ¾Ð²ÐµÐ½Ñ Ð°Ð¿Ð¾Ð¿ÑÐ¾Ð·Ð° Ñ Ð¸Ð½ÑÐ°ÐºÑÐ½ÑÑ ÐºÑÑÑ ÐÐ Ð¿Ð¾Ð²ÑÑÐµÐ½ Ð¾ÑÐ½Ð¾ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾ ÐºÑÑÑ ÐÐ¸ÑÑÐ°Ñ. ÐÑÐ¾Ð´ÑÐºÑÐ¸Ñ p53 Ð² ÐºÐ¾ÑÐµ Ð²Ð¸ÑÐ¾ÑÐ½Ð¾Ð¹ Ð´Ð¾Ð»Ð¸ Ð¼Ð¾Ð·Ð³Ð° Ð¿Ð¾Ð²ÑÑÐµÐ½Ð° Ñ Ð³ÑÑÐ¿Ð¿Ñ ÐÐ21+7, Ð¾ÑÐ½Ð¾ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾ Ð¸Ð½ÑÐ°ÐºÑÐ½ÑÑ ÐºÑÑÑ ÐÐ. Ð£ÑÐ¾Ð²ÐµÐ½Ñ CLOCK Ð² ÑÐ¾Ð¹ Ð¶Ðµ Ð¾Ð±Ð»Ð°ÑÑÐ¸ Ð¼Ð¾Ð·Ð³Ð° Ð¿Ð¾Ð²ÑÑÐµÐ½ Ñ ÐºÑÑÑ ÐÐ21+7 Ð² ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ð¸ Ñ ÐºÑÑÑÐ°Ð¼Ð¸ ÐÐ7+7. Ð£ÑÐ¾Ð²ÐµÐ½Ñ Ð°Ð¿Ð¾Ð¿ÑÐ¾Ð·Ð° ÑÐ²ÐµÐ»Ð¸ÑÐ¸Ð»ÑÑ Ñ Ð³ÑÑÐ¿Ð¿Ñ ÐÐ ÐºÐ¸Ð½Ð´Ð»Ð¸Ð½Ð³ 21 Ð´ÐµÐ½Ñ, Ð¾ÑÐ½Ð¾ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½ÑÑ ÐºÑÑÑ ÐÐ. ÐÑÐ¾Ð´ÑÐºÑÐ¸Ñ Nrf2 Ð² ÐºÐ¾ÑÐµ Ð²Ð¸ÑÐ¾ÑÐ½Ð¾Ð¹ Ð´Ð¾Ð»Ð¸ Ð¼Ð¾Ð·Ð³Ð° Ñ ÐºÑÑÑ Ð»Ð¸Ð½Ð¸Ð¸ ÐÐ Ð¾ÑÑÐ°ÐµÑÑÑ Ð½ÐµÐ¸Ð·Ð¼ÐµÐ½Ð½Ð¾Ð¹. Ð¢Ð°ÐºÐ¸Ð¼ Ð¾Ð±ÑÐ°Ð·Ð¾Ð¼, Ð¿Ð¾Ð½Ð¸Ð¶ÐµÐ½Ð½ÑÐ¹ ÑÑÐ¾Ð²ÐµÐ½Ñ p53 Ð¸ CLOCK Ñ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½ÑÑ ÐºÑÑÑ ÐÐ Ð³Ð¾Ð²Ð¾ÑÐ¸Ñ Ð¾ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð¾ÑÐ¾Ð±ÐµÐ½Ð½Ð¾ÑÑÑÑ Ð»Ð¸Ð½Ð¸Ð¸ Ð² Ð¿ÑÐ¾Ð´ÑÐºÑÐ¸Ð¸ ÑÑÐ¸Ñ Ð±ÐµÐ»ÐºÐ¾Ð². ÐÐ·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð² ÑÑÐ¾Ð²Ð½ÑÑ p53 Ð¸ CLOCK ÑÐ²Ð¸Ð´ÐµÑÐµÐ»ÑÑÑÐ²ÑÑÑ Ð¾ Ð²Ð¾Ð²Ð»ÐµÑÐµÐ½Ð¸Ð¸ ÑÐ¸ÑÐºÐ°Ð´Ð½ÑÑ ÑÐ¸ÑÐ¼Ð¾Ð² Ð¸ Ð°Ð¿Ð¾Ð¿ÑÐ¾Ð·Ð° Ð² ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¾Ð³ÐµÐ½ÐµÐ·. ÐÐºÑÐ¸Ð²Ð°ÑÐ¸Ñ Ð¿ÑÑÐ¸ Ð°ÐºÑÐ¸Ð¾ÐºÑÐ¸Ð´Ð°Ð½ÑÐ½Ð¾Ð¹ Ð·Ð°ÑÐ¸ÑÑ Ð²ÑÑÐ²Ð¸ÑÑ Ð½Ðµ ÑÐ´Ð°Ð»Ð¾ÑÑ. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð¼Ð¾Ð³ÑÑ Ð¿Ð¾Ð¼Ð¾ÑÑ Ð² Ð¿Ð¾Ð¸ÑÐºÐµ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½ÑÑ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð¾Ð², Ð²Ð¾Ð²Ð»ÐµÑÐµÐ½Ð½ÑÑ Ð² ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¾Ð³ÐµÐ½ÐµÐ·, Ð¸ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐµ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð»ÐµÑÐµÐ½Ð¸Ñ ÑÑÐ¾Ð³Ð¾ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ñ.
description: The subject of the graduate qualification work is: "Production of proteins, which take part in the regulation of apoptosis and circadian rhythms in the Krushinsky-Molodkina rat line." This work is devoted to the study of changes in the levels of proteins involved in the regulation of apoptosis, the sleep-wake cycle and protection against oxidative stress in the temporal cortex of the brain in audiogenic epilepsy using the Krushinsky-Molodkina rat model. For that, Krushinsky-Molodkina (KM) rats were underwent to audiogenic kindling during 7 and 21 days followed by 7 days of rest. We study the cortex of temporal lobe of the brain. We performed an inmunohistochemical reactions with antibodies to p53, CLOCK, Nrf2, and we detected the level of apoptosis. Next, we compared the optical density of stained cells and absolute number of apoptotic bodies in the temporal lobe of the brain of Krushinsky-Molodkina rats with similar indicators in intact rats of the same line and Wistar rats. We found that the levels of p53 and CLOCK are reduced in control KM rats compared to Wistar rats, while Nrf2 synthesis remains unchanged. The level of apoptosis in intact KM rats is lower than in Wistar rats. The production of p53 in the temporal cortex is increased in group KM21+7 relative to intact KM rats. The level of CLOCK in the same area of the brain is increased in KM21+7 rats compared to KM7+7 rats. The level of apoptosis increased in group KM kindling for 21 days, relative to control KM rats. Nrf2 production in the temporal cortex of KM rats remains unchanged. The obtained results can help in the search for molecular mechanisms involved in epileptogenesis and the development of methods for treating this diseasÐµ.