title: Ð Ð¾Ð»Ñ Ð²ÑÐ¾ÑÐ¸ÑÐ½Ð¾Ð¹ ÑÑÑÑÐºÑÑÑÑ NS-ÑÐµÐ³Ð¼ÐµÐ½ÑÐ° Ð² Ð¶Ð¸Ð·Ð½ÐµÐ½Ð½Ð¾Ð¼ ÑÐ¸ÐºÐ»Ðµ Ð²Ð¸ÑÑÑÐ° Ð³ÑÐ¸Ð¿Ð¿Ð° Ð
title: Ð½Ð°ÑÑÐ½ÑÐ¹ Ð´Ð¾ÐºÐ»Ð°Ð´
titleType: Subtitle
title: The role of NS segment RNA secondary structure in the influenza A viral life cycle
Creators: name: ÐÐ°ÑÐ°Ð½Ð¾Ð²ÑÐºÐ°Ñ, ÐÑÐ¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: Ð²Ð¸ÑÑÑ Ð³ÑÐ¸Ð¿Ð¿Ð° Ð
Ð²ÑÐ¾ÑÐ¸ÑÐ½Ð°Ñ ÑÑÑÑÐºÑÑÑÐ° Ð ÐÐ
Ð ÐÐ-ÑÐ¿Ð¸Ð»ÑÐºÐ¸
ÑÐµÐ³Ð¼ÐµÐ½Ñ NS
Ð±ÐµÐ»Ð¾Ðº NS1
Ð¿Ð°ÑÐ¾Ð³ÐµÐ½Ð½Ð¾ÑÑÑ
Ð¼ÐµÑÐ¾Ð´ Ð¾Ð±ÑÐ°ÑÐ½Ð¾Ð¹ Ð³ÐµÐ½ÐµÑÐ¸ÐºÐ¸
influenza A
RNA secondary structure
RNA-hairpin
NS segment
NS1 protein
pathogenicity
reverse genetics

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð²ÑÐ¾ÑÐ¸ÑÐ½ÑÑ ÑÑÑÑÐºÑÑÑ Ð ÐÐ Ð³ÐµÐ½Ð° NS Ð²Ð¸ÑÑÑÐ° Ð³ÑÐ¸Ð¿Ð¿Ð° ÐºÐ°Ðº Ð´Ð¾Ð¿Ð¾Ð»Ð½Ð¸ÑÐµÐ»ÑÐ½ÑÑ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð² Ð¿Ð°ÑÐ¾Ð³ÐµÐ½Ð½Ð¾ÑÑÐ¸, ÑÑÐ¾ Ð¿Ð¾Ð·Ð²Ð¾Ð»Ð¸Ð»Ð¾ Ð±Ñ ÑÐ°ÑÑÐ¼Ð°ÑÑÐ¸Ð²Ð°ÑÑ Ð¸Ñ Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð½Ð¾Ð²ÑÑ Ð¼Ð¸ÑÐµÐ½ÐµÐ¹ Ð´Ð»Ñ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ¸ Ð¿ÑÐ¾ÑÐ¸Ð²Ð¾Ð²Ð¸ÑÑÑÐ½ÑÑ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð² Ð½Ð¾Ð²Ð¾Ð³Ð¾ Ð¿Ð¾ÐºÐ¾Ð»ÐµÐ½Ð¸Ñ. Ð ÑÐ°Ð¼ÐºÐ°Ñ Ð´Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ñ 4 ÑÑÐ°Ð¼Ð¼Ð° Ð³ÑÐ¸Ð¿Ð¿Ð° Ð Ñ ÑÐ°Ð·Ð»Ð¸ÑÐ½Ð¾Ð¹ Ð²ÑÐ¾ÑÐ¸ÑÐ½Ð¾Ð¹ ÑÑÑÑÐºÑÑÑÐ¾Ð¹ Ð ÐÐ Ð² Ð¾Ð±Ð»Ð°ÑÑÑÑ (82-148 Ð½.) Ð¸ (487-564 Ð½.) Ð³ÐµÐ½Ð° NS. Ð ÑÐ°Ð±Ð¾ÑÐµ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÐµÐ½Ñ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾-Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð¸ ÑÐµÐ¿ÑÐ¾Ð´ÑÐºÑÐ¸Ð²Ð½ÑÑ ÑÐ²Ð¾Ð¹ÑÑÐ² Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð²Ð¸ÑÑÑÐ¾Ð² Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¼Ð¾Ð´ÐµÐ»ÐµÐ¹ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ ÐºÑÐ»ÑÑÑÑÐ°Ñ Ð¸ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ½ÑÑ Ð¶Ð¸Ð²Ð¾ÑÐ½ÑÑ . ÐÐ±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð²ÑÐ¾ÑÐ¸ÑÐ½Ð¾Ð¹ ÑÑÑÑÐºÑÑÑÑ (82-148 Ð½.) Ð½Ð° ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð²Ð¸ÑÑÑÐ½Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° NS1 Ð½Ð° ÑÐ°Ð½Ð½Ð¸Ñ ÑÑÐ°Ð¿Ð°Ñ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸. ÐÐ¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð²Ð¸ÑÑÑ, Ñ Ð°ÑÐ°ÐºÑÐµÑÐ¸Ð·ÑÑÑÐ¸Ð¹ÑÑ Ð¾ÑÑÑÑÑÑÐ²Ð¸ÐµÐ¼ Ð ÐÐ-ÑÐ¿Ð¸Ð»ÑÐºÐ¸ Ð² Ð¿Ð¾Ð»Ð¾Ð¶ÐµÐ½Ð¸Ð¸ (82-148) Ð¸ ÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾Ð¹ ÑÐ¿Ð¸Ð»ÑÐºÐ¾Ð¹ Ð² Ð¾Ð±Ð»Ð°ÑÑÐ¸ (497-564) Ð¼Ð ÐÐ Ð³ÐµÐ½Ð° NS, Ð¾Ð±Ð»Ð°Ð´Ð°ÐµÑ Ð±Ð¾Ð»ÐµÐµ Ð½Ð¸Ð·ÐºÐ¾Ð¹ Ð¿Ð°ÑÐ¾Ð³ÐµÐ½Ð½Ð¾ÑÑÑÑ Ð´Ð»Ñ Ð¼ÑÑÐµÐ¹ Ð¿Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ Ñ Ð´ÑÑÐ³Ð¸Ð¼Ð¸ Ð²Ð¸ÑÑÑÐ°Ð¼Ð¸.
description: This paper is devoted to the study of secondary RNA structures of the influenza virus NS gene as an additional pathogenicity factor, which may allow us to consider conserved RNA elements as new targets for the development of new generation antiviral drugs. Using a reverse genetics approach, four influenza virus strains were constructed featuring point mutations that provide various NS RNA structures (82-148 and 497-564 regions). The research presents results on the reproductive and molecular biological properties of the obtained viruses, demonstrated using different cell cultures and laboratory animals. An influence of a secondary structure (82-148 nt) on the expression of the viral NS1 protein has been found in the early stages of infection. It was shown that the virus characterized by the absence of an RNA hairpin in position (82-148) and a stable hairpin in the region (497-564) of NS gene mRNA has lower pathogenicity in mice, compared with other viruses.