cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Approaches to improving the therapeutic potential of Mesenchymal Stromal Cells
Creators: name: Gray, Andrea
Publisher: name: No Publisher Supplied
Year: 2015

description: Mesenchymal stromal cells (MSCs) hold great potential as a cellular therapy due in part to their tissue protective and regenerative abilities, achieved via the secretion of many bioactive molecules and induced by microenvironmental cues including soluble factors. However, translation of MSCs into the clinic, where variability exists amongst both MSC donors and recipients, has not resulted in dramatic success. Efforts to identify activation inputs that promote specific MSC phenotypes have been inconsistent and lack systematic optimization. The goal of our work is to address these hurdles using a combination of approaches. We aimed to (1) develop a systematic approach for optimizing activation parameters (type and/or combinations of activating molecule, concentration, and duration of exposure) that enhance MSC immunomodulation, (2) characterize MSCs treated with the resulting pre-activation protocol, and (3) test the performance of the pre-activation protocol using multiple MSC donors. In a high throughput in vitro screening assay designed using fractional factorial design of experiments, we identified interleukin (IL)-1β and lipopolysaccharide (LPS), individually and in combination, as optimal activators of MSC attenuation of pro-inflammatory macrophage tumor necrosis factor (TNF)-α production via prostaglandin E2 (PGE2) secretion. Further optimization of dose and duration of pre-activation, however, revealed that brief (1 hour) exposure of to 1 ng/mL IL-1β alone resulted in the highest sustained upregulation of PGE2 secretion post-activation. This pre-activation protocol generated MSCs whose PGE2 secretion was more sensitive to induction by secondary inflammatory molecules than MSCs with no pre-activation. IL-1β pre-activation led to enhanced MSC-mediated attenuation of macrophage TNF-α in a co-culture assay. This protocol was also successful in enhancing these functions for multiple MSC donors, although variability was noted in the absolute secretion levels of PGE2, the level of improvement in macrophage modulation, and the correlation of MSC PGE2 and macrophage TNF-α. Using the previous assays, we found that alginate encapsulated MSCs retained the ability to respond to activation factors. Statistical analysis revealed that macrophage TNF-α level was more significantly a function of PGE2 level, MSC activation factor, and macrophage donor rather than MSC culture format (monolayer versus encapsulated).
Sponge-associated dehalogenating microorganisms and isotope analysis of their dehalogenation of brominated phenols
Creators: name: Gray, Isabel Del Carmen
Publisher: name: No Publisher Supplied
Year: 2015

description: Sponges are filter feeders, ancient animals that have been extremely successful in surviving for over 600 million years almost unchanged. These metazoa can harbor microbes comprising almost 35% of their bodies at densities of 109 cells/g. Sponges are also rich in organohalides, usually with bioactive cytostatic and/or cytotoxic characteristics. Some of these organohalides resemble anthropogenic pollutants, such as halogenated dioxins and flame retardants. Thus, in nature some sponges contain both a high number of microbes as well as a high concentration of organohalides. This includes the sponge Aplysina aerophoba, from where Desulfoluna spongiiphila was isolated, a sulfate reducing Deltaproteobacteria that can reductively dehalogenate bromophenols. This observation led to the interest of enriching for sponge-associated microbes capable of dehalogenation from different ecoregions of the temperate and tropical oceans. The hypothesis is that dehalogenating bacteria are widespread among sponge species regardless of geographical location and form stable populations within the sponge animal that function in the cycling of organohalide compounds. Anaerobic dehalogenating activity was found to be widespread among sponges. In addition, new isolates obtained were closely related to D. spongiiphila. Compound Specific Isotope Analysis (CSIA) of 2,6-dibromophenol and its dehalogenation products revealed that carbon isotopic enrichment factors for sponge-associated bacterial dehalogenation are identical, probably due to a high similarity in the dehalogenating mechanism. The results support the hypothesis that dehalogenating microorganisms are widely distributed within marine sponges. The association between host sponge and associated microbiota is probably driven by the unique organohalide chemistry. At the same time the research of dehalogenating sponge-associated microorganism provided a unique setting for a deeper understanding into microbe-animal associations that could potentially be an ancient symbiosis
Doliops pachyrrhynchoides Heller 1916
Creators: name: Barševskis, Arvīds
name: Barševska, Zeltīte
name: Vanaga, Anarina
name: Borodina, Olga
name: Roze, Liāna
name: Pilace, Sanda
name: Mantilla, Leslae Kay Cataytay
Publisher: name: Zenodo
Year: 2021
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Coleoptera
Cerambycidae
Doliops
Doliops pachyrrhynchoides

description: 24. Doliops pachyrrhynchoides Heller, 1916
Philippines: Luzon isl., Aurora, Dingalan, 02.2020 (1, local collector leg.), 09.2020 (1, local collector leg.), 10.2020 (1, local collector leg.), 04.2021 (1, local collector leg.).
description: Published as part of Barševskis, Arvīds, Barševska, Zeltīte, Vanaga, Anarina, Borodina, Olga, Roze, Liāna, Pilace, Sanda & Mantilla, Leslae Kay Cataytay, 2021, To the knowledge of long-horned beetles (Coleoptera: Cerambycidae) of the Oriental Region (with new synonymy). Part 4, pp. 229-247 in Baltic Journal of Coleopterology 21 (2) on page 234, DOI: 10.5281/zenodo.10905476
Apanthesis leuce
Creators: name: Jong, Rienk De
Publisher: name: Zenodo
Year: 2017
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Lepidoptera
Nymphalidae
Apanthesis
Apanthesis leuce

description: leuce. Apanthesis leuce Scudder, 1889
Incertae sedis.
USA, Colorado, Teller County, Florissant; late Priabonian, late Eocene.
Depository: MCZH (holotype, 16354).
Published figures: Emmel et al. (1992: Fig. 1 /10); Scudder (1889: Pl. LII Figs. 12, 13).
One forewing only, with venation and design well preserved. Radial formula 1, 2, 3+(4+5), udc absent, M1, R3+(4+5) and mdc almost originating from the same point; ldc weak. According to Scudder, the radial branching is typical for the “tribe Vanessidi” (Nymphalidae), but I do not know what is so typical about it, except for the weak ldc. Comstock (1961: 181) observed a closer similarity to Limenitis, with which also the markings correspond with a uniformously brown hue with a submarginal band parallel to termen and consisting of light-coloured, slightly kidney-shaped spots, and vague spots between the band and termen in intervenal areas). Because Limenitis and Anaea share characters in the male genitalia, Comstock (1961) considered the discovery of Apanthesis leuce an indication of a northern origin of Anaea. This is, however, an unwarranted speculation. The venational arrangement could as well be plesiomorphic and is, for instance, also similar to the arrangement in the pierid genus Eronia. Euronia was considered a primitive member of the pierid tribe Pierini by Klots (1931) based on morphological grounds. Braby et al. (2006) concluded from a molecular analysis of the Pieridae, that Euronia was grouped in the Colotis group of genera, the first offshoot of the Pierinae. The weakness of ldc may be an argument in favor of Nymphalidae, but it remains to be seen whether in a fossil of some 35 Ma of age the weak ldc in, e.g., Limenitis, can be distinguished from the rather weak ldc in Eronia. The only reason for considering the fossil a member of the Nymphalidae is, therefore, the similarity in wing markings to some recent species, a rather weak argument. Emmel et al. (1992) followed Scudder's decision; they did not mention Comstock’s remarks. I think it is wise to consider the fossil as without clear affinities, as long as elements of the wing design remain unstudied in a phylogenetic context.
description: Published as part of Jong, Rienk De, 2017, Fossil butterflies, calibration points and the molecular clock (Lepidoptera: Papilionoidea), pp. 1-63 in Zootaxa 4270 (1) on page 31, DOI: 10.5281/zenodo.583183
Distributions and reactivities of phenolic antioxidants in various aggregation systems
Creators: name: Gu, Qing
Publisher: name: No Publisher Supplied
Year: 2015

description: Selecting the best antioxidant, AO, for a particular food application is still a major problem  in food science because AO e ciencies are determined by a variety of factors. One major  di culty in establishing a scale of AO e ciencies has been the lack of reliable methods for  determining AO distributions between the di erent regions of aggregated systems, which  arises from the physical impossibility of separating the interfacial region from the aqueous  or oil regions. We have developed a on-invasive" approach to estimate AO distributions in  various aggregated systems. The observed rate constant, kobs, for the reaction of a chemical  probe, 4-n-hexadecylbenzenediazonium ion (16-ArN+2), that is located in the interfacial  region of an aggregation system with an AO is measured by a chemical derivatization  method or directly by UV-Vis spectroscopy depending upon the system turbidity. The  kinetic data is interpreted by using a well established pseudophase kinetic model that was  originally developed for treating chemical reactivity in micelles and microemulsions, and  in this thesis we demonstrate that the model can be applied to nonionic emulsions, ionic  emulsions, and vesicles.  Chapter 1 is a general introduction on pertinent background information including dynamic  equilibrium in surfactant aggregates, basic assumptions of the pseusophase kinetic  model and logic of the chemical trapping method, and e ect of AO distributions on AO  e ciency. Chapter 2 describes the application of the pseudophase kinetic model to cationic  and anionic emulsions in the absence and presence of added salt to obtain estimates of the  partition constants of t-butylhydroquinone, TBHQ, between the oil and interfacial region,  PIO, and the aqueous and interfacial region, PIW, and the second-order interfacial rate constant,  kI, that is independent of AO distributions. Chapter 3 reports measurements of kobs  for 16-ArN+2 reacting with TBHQ in C12E6 nonionic emulsions of constant composition but  di erent droplet size distributions together with hydration number estimates for C12E6.  The results support the pseudophase model assumptiont that rate constants for reactions  in emulsions are insensitive to changes in droplet size and that the medium properties of  the interfacial region are virtually constant. Chapter 4 demonstrates that the AO reactivity  of a homologous series of gallate esters as characterized by the observed rate constant,  kobs, for their reactions with 16-ArN+2 plateaus in vesicular solutions once the AOs are fully  associated with the vesicles. This plateau di ers from the cuto  e ect" observed in oil-inwater  emulsions, the AO activity increases with the alkyl chain length of AO and reaches a  maximum at an intermediate chain length, after which further increase in AO chain length  results in a decrease in activity.
Additional file 8: of Differentiated transcriptional signatures in the maize landraces of Chiapas, Mexico
Creators: name: Kost, Matthew
name: Perales, Hugo
name: Saranga Wijeratne
name: Asela Wijeratne
name: Stockinger, Eric
name: Mercer, Kristin
Publisher: name: Figshare
Year: 2017
Subjects: subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Molecular Biology
subject: Biotechnology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: Plant Biology
subject: Computational Biology

description: Co-expression module gene counts. (DOC 43Â kb)
Next generation sequencing to study organelle genome structure and function
Creators: name: Gurdon, Csanad
Publisher: name: No Publisher Supplied
Year: 2015

description: I utilized next generation sequencing in three separate studies to study organelle genome structure and function in maize, tobacco and barrel medic.  In the first study, I found cell-to-cell movement of mitochondria through a graft junction in an experiment designed to select for chloroplast transfer from Nicotiana sylvestris into N. tabacum cells. Flowers of N. tabacum are cytoplasmic male sterile (CMS) due to a sterility-causing mitochondrial genome, thus, N. sylvestris mitochondrial DNA transfer was recognized by restoration of flower fertility. Extensive recombination of mitochondrial genome sequences was revealed in a graft regenerant with fertile flowers, linking male sterility to a mitochondrial gene causing homeotic conversion of anthers (hca). Cell-to-cell movement of mitochondrial DNA could enable introduction of male-sterility causing sequences into graft compatible species, and could be used to create novel forms of CMS in species where it does not exist.  In the second study, I sequenced and compared the plastid genomes of three fertile and three CMS maize lines: cms-C, cms-S, and cms-T. The plastid genomes were found to harbor little diversity.  I also report that 27 cms-T accessions representing five independently isolated lines have the same diagnostic plastid SNPs. This indicates a single origin and maternal co-transmission of the cms-T mitochondria and plastids to the seed progeny, excluding exceptional pollen transmission of mitochondria or multiple horizontal gene transfer events as the source of the CMS-causing gene.  In the third study, I found two distinct plastid genome configurations in Medicago truncatula. I sequenced and assembled the plastid genome of four accessions, three of which belong to ssp. truncatula, and the fourth, R108, to ssp. tricycla. R108 plastid DNA had a ~45-kb inversion compared to ssp. truncatula, mediated by a short, imperfect repeat. DNA gel blot analyses of seven additional ssp. tricycla accessions detected only one of the two alternative genome arrangements. Furthermore, a variable number of repeats was found in the essential accD and ycf1 coding regions. accD length was distinct in ten M. truncatula ecotypes, ranging between 650 and 796 amino acids. Thus, intra-species plastid genome variability in M. truncatula could be linked to repeat-mediated genome rearrangements.
Stolopsyche libytheoides
Creators: name: Jong, Rienk De
Publisher: name: Zenodo
Year: 2017
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Lepidoptera
Pieridae
Stolopsyche
Stolopsyche libytheoides

description: libytheoides. Stolopsyche libytheoides Scudder, 1889
Family incertae sedis.
USA, Colorado, Teller County, Florissant; late Priabonian, late Eocene.
Depository: MCZH (holotype, no.11077).
Published figures: Scudder (1889: Pl. LIII Figs. 1–3); Emmel et al. (1992: Fig. 4 /1); Murata (1998: Figs. 42, 43).
This specimen is poorly preserved; according to Zeuner (1961) this fossil is too poor for attempting an assignment to a particular family. Antennae with very gradually thickened club, which takes about 1/3 of the whole antennal length. If not for this character, the fossil could as well represent a non-papilionoid lepidopteran. Forelegs long, only a little shorter than middle legs. This is a plesiomorphic character found in Papilionidae, Pieridae, Lycaenidae females, Libythea females, and Hesperiidae. It could place the fossil in the ancestry of any butterfly family. Since the head is small for Hesperiidae and Libythea, the forewing is long for Lycaenidae (ca. 25 mm), and the palps long for Papilionidae, Scudder thought the Pieridae the most likely family for the fossil. Apparently the fossil has too few characters preserved to be of any use for phylogenetic research. I cannot find any evidence supporting the claim by Emmel et al. (1992) that the fossil “was closer to Pieris than any other living genus.”
Apparently, Braby et al. (2006) based their use of this fossil as calibration point in the phylogenetic tree of Pieridae close to the origin of Pieris on the unsupported assumption of Emmel et al. (1992) (l.c.)—an unwarranted and probably erroneous assumption.
description: Published as part of Jong, Rienk De, 2017, Fossil butterflies, calibration points and the molecular clock (Lepidoptera: Papilionoidea), pp. 1-63 in Zootaxa 4270 (1) on page 31, DOI: 10.5281/zenodo.583183
Examination of major tea catechins on GLUT4 translocation in L6 skeletal muscle cells
Creators: name: Hamlin, Nicole
Publisher: name: No Publisher Supplied
Year: 2015

description: Obesity and its manifested pathophysiological effects (insulin-resistance, metabolic disease, type 2 diabetes, cardiovascular disease, etc.) are reaching epidemic proportions in the developed world.  Roughly one-third of adults twenty years and over are considered overweight or obese, therefore interventional approaches have been of interest in the scientific community. Besides its palatable popularity, tea brewed from the leaves of the plant Camellia sinensis has become increasingly popular due to its possible effects on human health in alleviating complications that arise from diseases such as metabolic syndrome, diabetes, cardiovascular disease, and various types of cancers. These beneficial qualities have mainly been attributed to catechins, compounds found in tea leaves that account for 30-42% of the water-extractable solids.   In the present study, I investigated major tea catechins epigallocatechin-3-O-gallate (EGCG), epigallocatechin (EGC), epicatechin-3-O-gallate (ECG), and  epicatechin (EC), as well as gallic acid (GA) on L6 rat skeletal muscle cells in order to test their ultimate effects on glucose transporter 4 (GLUT4) translocation via activation of two distinct signaling pathways implicated in the process: (1) the insulin-mediated phosphoinositide 3-kinase (PI3K) pathway and (2) the insulin-independent AMPK  pathway. Treatment of cells with the catechins resulted in Thr308 phosphorylation of Akt (a key protein in the PI3K pathway that facilitates GLUT4 translocation). Activation was stronger with the gallated catechins (EGCG and ECG) than the non-gallated catechins (EGC and EC) and gallic acid. Similar results were observed in AMPK activation. Furthermore, GLUT4 translocation from the cytosol to the plasma membrane was higher in samples treated with gallated catechins than cells treated with other polyphenols. Together, these data suggest that major polyphenols found in tea promote translocation of the GLUT4 protein in skeletal muscle. The effectiveness of the catechins on GLUT4 translocation may be attributable to their abilities to generate reactive oxygen species (ROS), decrease intracellular ATP levels, or their abilities to interact with components on the outer leaflet of the plasma membrane, but these possible mechanisms remain to be investigated.
Reparation des traumatismes individuels, familiaux et communautaires
Creators: name: Jandarme Florentin
name: Ozowa-Latem Josué
Publisher: name: Zenodo
Year: 2024

description: Les guerres récurrentes à l'Est de la Rdc ont causé beaucoup de préjudices à la communauté. Et parmi lesquels, on enregistre les violences sexuelles faites aux femmes et aux enfants. Les conséquences cette tragédie humaine enfreignent à la culture de la population de l’est de la RDC, et génère des traumatismes psychiques. Pour leur prise en charge, les survivants ont besoin d'une réparation au niveau individuel, familial et communautaire. Ce dernier aspect de la prise en charge des survivant des violences sexuelles tient en compte la culture des concernés. Ainsi, cet article relève l’importance de la prise en charge holistique des femmes traumatisées à cause de la violence sexuelle.
Formation of enriched black tea extract loaded chitosan nanoparticles via electrospraying
Creators: name: Hammond, Samuel James
Publisher: name: No Publisher Supplied
Year: 2015

description: Creating nanoparticles of beneficial nutraceuticals and pharmaceuticals has had a large surge of research due to the enhancement of absorption and bioavailability by decreasing their size. One of these ways is by electrohydrodynamic atomization, also known as electrospraying. In general, this novel process is done by forcing a liquid through a capillary nozzle and which is subjected to an electrical field. While there are different ways to create nanoparticles, the novel method of electrospraying can be beneficial over other types of nanoparticle formation. Reasons include high control over particle size and distribution by altering electrospray parameters (voltage, flow rate, distance, and time), higher encapsulation efficiency than other methods, and also it is a one step process without exposure to extreme conditions (Gomez-Estaca et. al. 2012, Jaworek and Sobcyzk 2008). The current study aimed to create a chitosan encapsulated theaflavin-2 enriched black tea extract (BTE) nanoparticles via electrospraying. The first step of this process was to create the smallest chitosan nanoparticles possible by altering the electrospray parameters and the chitosan-acetic acid solution parameters. The solution properties altered include chitosan molecular weight, acetic acid concentration, and chitosan concentration. Specifically, the electrospray parameters such as voltage, flow rate and distance from syringe to collector are the most important in determining particle size. After creating the smallest chitosan particles, the TF-2 enriched black tea extract was added to the chitosan-acetic acid solution to be electrosprayed. The particles were assessed with the following procedures: Atomic force microscopy (AFM) and scanning electron microscopy (SEM) for particle morphology and size, and loading efficiency with ultraviolet–visible spectrophotometer (UV-VIS). Chitosan-BTE nanoparticles were successfully created in a one step process. Diameter of the particles on average ranged from 255 nm to 560 nm. Encapsulation efficiency was above 95% for all but one sample set. Future work includes MTT assay and cellular uptake.
Additional file 8: of Differentiated transcriptional signatures in the maize landraces of Chiapas, Mexico
Creators: name: Kost, Matthew
name: Perales, Hugo
name: Saranga Wijeratne
name: Asela Wijeratne
name: Stockinger, Eric
name: Mercer, Kristin
Publisher: name: Figshare
Year: 2017
Subjects: subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Molecular Biology
subject: Biotechnology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Plant Biology
subject: Computational Biology

description: Co-expression module gene counts. (DOC 43Â kb)
Papilio maackii
Creators: name: Jong, Rienk De
Publisher: name: Zenodo
Year: 2017
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Lepidoptera
Papilionidae
Papilio
Papilio maackii

description: maackii. Papilio cf. maackii Ménétries, 1859
Papilionidae: Papilioninae: Papilio (?).
Japan, Tochigi Pref., Shiobara; late Pleistocene, 37,000 BP (see Fujiyama 1983).
Depository: NSMT (one specimen, no. 7141).
Published figures: Fujiyama (1968: Fig. 1, Pl. 1 Fig. 1); illustrations of the extant species can be found in many books on East Asian butterflies.
Reported by Fujiyama (1968). A forewing only is present, of which almost half is missing. Two papilionid apomorphies present: crossvein Cu-A1 (but see above, Venational patterns of the forewing) and 2A terminating on dorsum. Radial formula 1, 2, 3, 4+5, R3 and the common stem of R4 and R5, both originating from the upper corner of the cell. After comparison with extant papilionine species in Japan and adjacent areas, Fujiyama concluded on the basis of overall similarity (as far as overall similarity is possible with only half a wing), but principally determined by the proportions of the cell, that the fossil falls within the variation of the extant species Papilio maackii. However, Fujiyama (1968) does not make clear that the fossil does not fall within the variation of other Papilio species as well. The identification by Fujiyama is based on comparison with the extant fauna of the region, not on apomorphies, and, thus, is questionable. In view of its relatively recent age, the fossil is not of interest for calibration purposes. Papilio maacki has a wide distribution in East Asia. The fossil was found in a lake deposit, which presumably formed when it was 5–5.5 0 C colder than at present, corresponding with the last ice age.
description: Published as part of Jong, Rienk De, 2017, Fossil butterflies, calibration points and the molecular clock (Lepidoptera: Papilionoidea), pp. 1-63 in Zootaxa 4270 (1) on page 32, DOI: 10.5281/zenodo.583183
Doliops pinedai Vives 2012
Creators: name: Barševskis, Arvīds
name: Barševska, Zeltīte
name: Vanaga, Anarina
name: Borodina, Olga
name: Roze, Liāna
name: Pilace, Sanda
name: Mantilla, Leslae Kay Cataytay
Publisher: name: Zenodo
Year: 2021
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Coleoptera
Cerambycidae
Doliops
Doliops pinedai

description: 25. Doliops pinedai Vives, 2012
Philippines: Luzon isl., Aurora, Dingalan, 12.2018 (3, local collector leg.), 02.2019 (1, local collector leg.), 05.2020 (1, local collector leg.), 12.2020 (1, local collector leg.).
description: Published as part of Barševskis, Arvīds, Barševska, Zeltīte, Vanaga, Anarina, Borodina, Olga, Roze, Liāna, Pilace, Sanda & Mantilla, Leslae Kay Cataytay, 2021, To the knowledge of long-horned beetles (Coleoptera: Cerambycidae) of the Oriental Region (with new synonymy). Part 4, pp. 229-247 in Baltic Journal of Coleopterology 21 (2) on page 234, DOI: 10.5281/zenodo.10905476
Purifications of small molecules that regulate neuronal cell-lineage specification
Creators: name: Hamod, Aula
Publisher: name: No Publisher Supplied
Year: 2015

description: There are certain important factors that are essential for specification, development and maintenance vertebrate nervous system. During development these factors regulate axonal growth, dendrite pruning, cell fate decision and different other neuronal functions.  In adult nervous system, they modulate synaptic activity and regulate neuronal connectivity. Cultivation of mammalian cells for production of recombinant protein has wide scientific and clinical applications because it offers many advantages especially the posttranslational modification. Human embryonic kidney (HEK) 293 cell lines expressing gene of interests will be constructed using calcium phosphate transfection of expression vectors. I have constructed stable cell lines for expressing large quantity of stable neurotrophic and neurological fate determinant factors including: Brain derived neurotrophic factor (BDNF),Glial derived neurotrophic factor (GDNF), Neurotrophin 3(NT3), Noggin and Sonic the hedgehog (SHH).This recombinant proteins were successfully purified by affinity chromatography and are tested on the conversion of induced pluripotent stem (iPS) cells into specific type of neurons, we found that some of the purified proteins are capable of support neuronal differentiation. These neurons have complex morphology, mature synaptic marker such as synapsin, and exhibit mature membrane properties and synaptic activity. We conclude that mammalian cell produced recombinant factors can be used to regulate human neuronal maturation and cell type specification.
Deliverable 7.1: Legal, ethical, and regulatory inventory
Creators: name: Kamenjasevic, Erik
name: Biasin, Elisabetta
name: Dell'Aglio, Daniele
name: Romanovych, Anna
Publisher: name: Zenodo
Year: 2024

description: The HEREDITARY project represents an ambitious and multi-faceted approach to address the integration of complex medical data to enhance disease prevention, diagnosis, and treatment. In an era where digitalisation and machine learning promise transformative advances in healthcare, substantial legal, ethical and societal challenges persist, also due to the inherently complex, multimodal nature of health data and the regulatory frameworks governing it. HEREDITARY tackles these challenges by aiming to build a secure, interoperable infrastructure to link diverse health data sources across disease domains, centring on diseases involving the gut-brain axis – neurological diseases and gut microbiome-related disorders.

The main barriers to health data integration include technical and legal complexities that limit the potential of these data sources. These variations challenge interoperability and data consistency, obstructing the development of seamless, large-scale data analysis systems. Furthermore, legal frameworks such as the EU’s GDPR introduce essential but challenging requirements, adding complexity to cross-border and multicentre collaborations.

The deliverable analysis shows that the development of HEREDITARY must be guided by legal and ethical standards. The most relevant are the GDPR, cybersecurity legislation, EHDS proposal, NIS 2 Directive, and the evolving AI regulatory landscape, including the AI Act. Each of these legislation mandates robust data protection, security, and privacy measures, as well as guidelines for ethical AI deployment. HEREDITARY’s legal framework shall ensure that personal data use aligns with these requirements, fostering trusted, transparent, and ethical use of health data in clinical and research contexts. Next to these requirements, the ethical framework can help in finding the most suitable interpretative solutions in those areas where the law is imprecise, vague, or simply non-existent. Four main principles of biomedical ethics and the principles for the development of trustworthy AI systems are crucial for the HEREDITARY project.

Both the legal and ethical frameworks mentioned above and further detailed in this deliverable lay the ground for the research in forthcoming deliverables of WP7 ‘Legal, Ethical and Regulatory Frameworks’.
Additional file 9: of Differentiated transcriptional signatures in the maize landraces of Chiapas, Mexico
Creators: name: Kost, Matthew
name: Perales, Hugo
name: Saranga Wijeratne
name: Asela Wijeratne
name: Stockinger, Eric
name: Mercer, Kristin
Publisher: name: Figshare
Year: 2017
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Molecular Biology
subject: Biotechnology
subject: Ecology
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Inorganic Chemistry
subject: FOS: Chemical sciences
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: Plant Biology
subject: Computational Biology

description: GO enrichment analysis for the turquoise (A) and yellow (B) co-expression modules. (DOC 41Â kb)
Application of the electroosmotic effect for thrust generation
Creators: name: Hansen, Thomas Edward
Publisher: name: No Publisher Supplied
Year: 2015

description: The present work focuses on demonstrating the capabilities of electroosmotic pumps, (EOP) to generate thrust. An underwater glider was successfully propelled by electroosmosis for the first time published - at 0.85 inches per second. Asymmetric AC voltage pulsing proved to produce higher flow rates then equivalent DC pumps for the same average voltage. Ultra-short pulsing proved 100 nanosecond rise times in EOP are possible, which surpassed published predictions by three orders of magnitude. Theories behind efficiency losses of high power EOP were investigated. Direct measurement of effective voltage at the face of a membrane is the most accurate way to determine voltage drop across the electrolyte of an EOP. Forced convection lowered efficiency of the EOP for low voltages by preventing capacitance charging, but proved to prolong pump life during high power application.
"The eternal nation does not fear a long road"
Creators: name: Harel, Assaf
Publisher: name: No Publisher Supplied
Year: 2015

description: This is an ethnography of Jewish settlers in Israel/Palestine. Studies of religiously motivated settlers in the occupied territories indicate the intricate ties between settlement practices and a Jewish theology about the advent of redemption. This messianic theology binds future redemption with the maintenance of a physical union between Jews and the “Land of Israel.” However, among settlers themselves, the dominance of this messianic theology has been undermined by postmodernity and most notably by a series of Israeli territorial withdrawals that have contradicted the promise of redemption. These days, the religiously motivated settler population is divided among theological and ideological lines that pertain, among others issues, to the meaning of redemption and its relation to the state of Israel.    This dissertation begins with an investigation of the impact of the 2005 Israeli unilateral withdrawal from the Gaza Strip upon settlers and proceeds to compare three groups of religiously motivated settlers in the West Bank: an elite Religious Zionist settlement, settlers who engage in peacemaking activities with Palestinians, and settlers who act violently against Palestinians. Through a comparison of these different groups, this dissertation demonstrates that while messianism remains a central force in the realities of Jewish settlements and the Israeli-Palestinian conflict, it exists these days in more diversified forms than before. In addition, this ethnography illustrates how religion both underlies and undermines differences between Israelis and Palestinians and argues that local communities and religious leaders should be included in peace processes. Finally, by examining how messianic conceptions of time among different groups of Jewish settlers connect to their settlement practices, this study reveals the Israeli-Palestinian conflict to be as much about time as it is about space. Accordingly, this dissertation has broader implications for understanding the contemporary role of religion and time within the Israeli-Palestinian conflict and the political struggles of the Middle East.
Wuel Jian & Xing, 2022, nom. nov.
Creators: name: Jian, Qian
name: Xing, Jichun
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hemiptera
Asterolecaniidae
Wuel

description: Genus Wuel nom. nov.
Elagatis Wu 2006: 43 (Mandibulata: Insecta: Hemiptera: Asterolecaniidae), preoccupied by Elagatis Bennett 1840: 283 (Chordata: Actinopterygii: Carangiformes: Carangidae).
Type species: Elagatis tongzaense Wu 2006: 43.
Remarks on nomenclatural change. Wu (2006) established the genus Elagatis with the type species Elagatis tongzaense Wu 2006. The genus is a valid name in Hemiptera: Coccomorpha: Asterolecaniidae (García Morales et al. 2016); it includes only the type species, known only from China.
Unfortunately, the name Elagatis Wu 2006 is preoccupied by Elagatis Bennett 1840, a fish genus (Chordata: Actinopterygii: Carangiformes: Carangidae) based on the type species Elagatis bipinnulatus Bennett 1840. Thus, Elagatis Wu 2006 is a junior homonym of Elagatis Bennett 1840. According to Article 60 of the International Code of Zoological Nomenclature, a new replacement name is proposed for Elagatis Wu 2006: Wuelagatis Jian & Xing nom. nov.
Etymology. The replacement genus name is derived from S.J. Wu, the author of the junior homonym, combined with the pre-existing name Elagatis; a noun, gender masculine.
Summary of nomenclatural changes:
Wuelagatis Jian & Xing nom. nov. (rep. nov.) = Elagatis Wu 2006 (nec Bennett 1840)
Wuelagatis tongzaense (Wu 2006) comb. nov. = Elagatis tongzaense Wu 2006
description: Published as part of Jian, Qian & Xing, Jichun, 2022, Two new replacement names for genera in the Coccomorpha (Hemiptera: Sternorrhyncha) due to homonymy, pp. 149-150 in Zootaxa 5104 (1) on page 149, DOI: 10.11646/zootaxa.5104.1.10, http://zenodo.org/record/6257695
Doliops serapavginae Barsevskis 2014
Creators: name: Barševskis, Arvīds
name: Barševska, Zeltīte
name: Vanaga, Anarina
name: Borodina, Olga
name: Roze, Liāna
name: Pilace, Sanda
name: Mantilla, Leslae Kay Cataytay
Publisher: name: Zenodo
Year: 2021
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Coleoptera
Cerambycidae
Doliops
Doliops serapavginae

description: 26. Doliops serapavginae Barševskis, 2014
(Fig. 5)
Philippines: Mindanao isl., Sarangani, Kiamba, 10.2019 (3, local collector leg.).
description: Published as part of Barševskis, Arvīds, Barševska, Zeltīte, Vanaga, Anarina, Borodina, Olga, Roze, Liāna, Pilace, Sanda & Mantilla, Leslae Kay Cataytay, 2021, To the knowledge of long-horned beetles (Coleoptera: Cerambycidae) of the Oriental Region (with new synonymy). Part 4, pp. 229-247 in Baltic Journal of Coleopterology 21 (2) on page 235, DOI: 10.5281/zenodo.10905476
Arterial hemodynamics and its interaction with the left ventricle in heart failure
Creators: name: Hegyi, Gina N.
Publisher: name: No Publisher Supplied
Year: 2015

description: Monitoring heart-arterial system (LV-AS) interactions of cardiovascular patients is important in achieving successful diagnosis and treatment. This is particularly true in the cases of hypertension and heart failure. However, there has been limited quantitative hemodynamic analysis aimed at improving the outcome for the 70-million hypertensive patients and millions of heart failure patients.  This thesis develops a model-based strategy to provide a comprehensive evaluation of the state of the vascular system and its coupling to the heart, and ultimately aims to distinguish patient groups of heart failure with preserved or reduced ejection fraction (HFpEF and HFrEF).    The first aspect of this study is analysis of arterial hemodynamics from animal experimental data collected during normotensive, vasodilation, and induced hypertension conditions. Total arterial compliance (C), peripheral resistance (Rs), and aortic characteristic impedance (Z0) based on the 3-element Windkessel model were obtained, together with frequency-domain impedance, wave reflection information, and parameters for assessing global cardiac function. Subsequently, clinical human data were collected and analyzed, focusing on the LV-AS interactions. We identified critical parameters in LV-AS interaction, i.e. effective arterial system elastance, Ea, LV systolic elastance, Emax, LV-AS coupling index, k (=Ea/Emax), and C.     Results characterized hypertension by a significantly reduced C and increased Rs, confirmed by increased vascular impedance and wave reflections. Unloading the heart reversed these observations via vasodilator treatment. For human studies, we found C to be more effective than Ea in distinguishing groups of HFpEF and HFrEF patients. It was found that HFpEF patients tended to have a relatively independent coupling index, k, with respect to arterial parameter changes.    The findings in this work can be incorporated into larger scale clinical studies that implement the use of pulse waveform analysis and the identified critical LV-AS interaction parameters to improve patient treatment and outcome.
Life with, after, or beyond cancer
Creators: name: Hemler, Jennifer R.
Publisher: name: No Publisher Supplied
Year: 2015

description: I examine how women “move on,” or are unable to, after a diagnosis of breast cancer. I interviewed 80 survivors of various types and stages of breast cancer to explore the relationship between how breast cancer survivors think about cancer and how they manage the daily consequences of this disease, including its effects on identity. My main objective was to examine the cognitive strategies and social practices survivors employ for living with (a history of having had) cancer. Cancer experience is undoubtedly shaped by factors like disease stage and type, treatments received, time since diagnosis and treatment, age, and social location. But many of my participants, across categories, described bracketing some aspects of their experience while holding onto a certain degree of ontological insecurity as they redefined their lives after, with, or beyond cancer. Drawing on ontological insecurity enabled them to remain attuned to their selves: they used cancer to help them redraw boundaries in their lives and focus attention on their selves in ways they were not doing previously. While self-regulatory health practices, feminist ideologies/the women’s health movement, and environmental awareness overlap in breast cancer survivorship to produce activated patients and actualized subjectivities, my participants also discussed how the existential and medical uncertainties of their experiences led them to create new spaces for meaning in their lives. However, while many of my participants wanted to use cancer as a catalyst for self-growth or change across life domains, certainly not all of them were able to do so. Survivorship programs are critical in this regard. They can help survivors harness the uncertainty they feel instead of allowing it to become paralyzing or debilitating. Many survivors need help framing ontological insecurity as a resource to employ, not something to move beyond; but, moreover, they need spaces in which they can acknowledge these uncertainties as part of their new realities.
Additional file 9: of Differentiated transcriptional signatures in the maize landraces of Chiapas, Mexico
Creators: name: Kost, Matthew
name: Perales, Hugo
name: Saranga Wijeratne
name: Asela Wijeratne
name: Stockinger, Eric
name: Mercer, Kristin
Publisher: name: Figshare
Year: 2017
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Molecular Biology
subject: Biotechnology
subject: Ecology
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Inorganic Chemistry
subject: FOS: Chemical sciences
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Plant Biology
subject: Computational Biology

description: GO enrichment analysis for the turquoise (A) and yellow (B) co-expression modules. (DOC 41Â kb)
Lethe montana
Creators: name: Jong, Rienk De
Publisher: name: Zenodo
Year: 2017
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Lepidoptera
Nymphalidae
Lethe
Lethe montana

description: montana. Lethe montana Miller, Miller & Ivie, 2012
Nymphalidae: Satyrinae.
USA, Montana, Canyon Ferry Reservoir; Rupelian, early Oligocene.
Depository: holotype, Museum of Rockies, Montana State University, Bozeman (part and counterpart, MOR No. MV-152).
Published figures: CoBabe et al. (2002, Fig. 4 c), Miller et al. (2012: Figs 1–5.
The fossil consists of a well preserved part and counterpart of a forewing. In their diagnosis the authors state that “… the unbranched forewing radial veins … indicate thet this fossil is … not a member of the Hesperioidea.” Rather, this should read “the branched forewing radial veins”, but the conclusion is correct. Moreover, the absence from the cubital spur and vein 3A eliminates Papilionidae from consideration. The authors also eliminate Pieridae, since M1 branches off the cell, although branching of M1 off R3 is widespread in Pieridae but not universal. The remark that Riodinidae or Lycaenidae can be eliminated because the “… veins are not simple in that they do not arise from the base or from the radius or cubitus proper …”, is not clear to me. The authors arrive at the conclusion that the fossil belongs to the Nymphalidae, and since the forewing cell is closed, the subcostal vein is thickened at its base and there is a prominent ocellus in M1-M2, assignment to the Satyrinae seems obvious. The only problem is, that the basal part of the wing with the swelling of the subcostal vein, as represented in their reconstruction in Fig. 5, is absent from the fossil (Fig. 1) and from the interpretation of the fossil in Fig. 2.
See also the discussion under Lethe (?) corbieri on the absence of a basal swelling in Lethe. There is no convincing evidence for treating Lethe montana differently than Lethe (?) corbieri as far as its use as calibration point concerns. They are approximately the same age.
See also under Biogeographic considerations.
description: Published as part of Jong, Rienk De, 2017, Fossil butterflies, calibration points and the molecular clock (Lepidoptera: Papilionoidea), pp. 1-63 in Zootaxa 4270 (1) on page 32, DOI: 10.5281/zenodo.583183
Additional file 1: Table S1. of Long-term patient reported outcomes following radiation therapy for oropharyngeal cancer: cross-sectional assessment of a prospective symptom survey in patients â Ľ65Â years old
Creators: name: Eraj, Salman
name: Jomaa, Mona
name: Crosby Rock
name: Mohamed, Abdallah
name: Smith, Blaine
name: Smith, Joshua
name: Browne, Theodora
name: Cooksey, Luke
name: Bowman Williams
name: Temple, Brandi
name: Preston, Kathryn
name: Aymard, Jeremy
name: Gross, Neil
name: Weber, Randal
name: Hessel, Amy
name: Ferrarotto, Renata
name: Phan, Jack
name: Sturgis, Erich
name: Ehab Hanna
name: Frank, Steven
name: Morrison, William
name: Goepfert, Ryan
name: Lai, Stephen
name: Rosenthal, David
name: Mendoza, Tito
name: Cleeland, Charles
name: Hutcheson, Kate
name: Fuller, Clifton
name: Garden, Adam
name: G. Gunn
Publisher: name: Figshare
Year: 2017
Subjects: subject: Biotechnology
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Cancer
subject: Science Policy

description: Proportions of patients reporting moderate to severe (â Ľ5) rating for the 22 MDASI-HN symptom items by clinical subgroups of interest. (DOCX 20Â kb)
Novmammilla Jian & Xing, 2022, nom. nov.
Creators: name: Jian, Qian
name: Xing, Jichun
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Chordata
Insecta
Hemiptera
Aclerdidae
Novmammilla

description: Genus Novmammilla nom. nov.
Mammilla Wu 2011: 241 (Mandibulata: Hexapoda: Hemiptera: Aclerdidae), preoccupied by Mammilla Schumacher 1817: 190 (Mollusca: Gastropoda: Littorinimorpha: Naticidae).
Type species: Mammilla jinzhaiensis Wu 2011: 241–244.
Remarks on nomenclatural change. Wu (2011) established the genus Mammilla with the type species Mammilla jinzhaiensis Wu 2011. It is a valid genus in Hemiptera: Aclerdidae (García Morales et al. 2016; Normark et al. 2019) that was previously placed in the family Diaspididae. The genus is monotypic and is known only from China.
Unfortunately, the name Mammilla Wu 2011 is preoccupied by Mammilla Schumacher 1817, a marine snail genus belonging to Mollusca: Gastropoda: Littorinimorpha: Naticidae, based on the type species Mammilla fasciata Schumacher 1817. Thus, Mammilla Wu 2011 is a junior homonym of Mammilla Schumacher 1817. According to Article 60 of the International Code of Zoological Nomenclature, a new replacement name for Mammilla Wu 2011 is proposed: Novmammilla Jian & Xing nom. nov.
Etymology. The replacement name is derived from the pre-existing name Mammilla, combined with the prefix “ nov - ” from the Latin “ novus ” meaning new; gender feminine.
description: Published as part of Jian, Qian & Xing, Jichun, 2022, Two new replacement names for genera in the Coccomorpha (Hemiptera: Sternorrhyncha) due to homonymy, pp. 149-150 in Zootaxa 5104 (1) on pages 149-150, DOI: 10.11646/zootaxa.5104.1.10, http://zenodo.org/record/6257695