Additional file 1 of Effects of nitrogen addition and drought on the relationship between nitrogen- and water-use efficiency in a temperate grassland
Creators: name: Hu, Jiaxin
name: Ma, Wang
name: Wang, Zhengwen
Publisher: name: figshare
Year: 2024
Subjects: subject: Ecology
subject: FOS: Biological sciences
subject: Plant Biology

description: Additional file 1: Figure S1. The geographical location of the research site (a), the randomized block design of the study (b) and the picture of rainout shelters used in the manipulative drought experiment (c). In Fig. S1a, the rectangular frame in the map of China is the territory of Hulunbuir City, Inner Mongolia, and the solid circle in the map of Hulunbuir City shows the location of the research site. Fig. S1b shows the six blocks of the experimental platform, each containing eight different treatments (C: control, N: N addition, D: drought, S: snowmelt delay, ND: combination of drought and N addition, NS: combination of N addition and snowmelt delay, DS: combination of drought and snowmelt delay, NDS: combination of drought, N addition and snowmelt delay). Figure S2. The relative change of WUEINST (a–f) of the six studied species induced by N addition and drought. Data are shown as mean ± SE. Among them, panel a and d show the changes in grasses, panel b and e show the changes in the leguminous forb, panel c and f show the changes in non-leguminous forbs. “N–C” and “ND–D” represent the relative changes caused by N addition without and with drought, respectively; “D–C” and “ND–N” represent the relative changes caused by drought without and with N addition, respectively. ^0.05 &lt; p &lt; 0.1, *0.01 &lt; p &lt; 0.05, **0.001 &lt; p &lt; 0.01, ***p &lt; 0.001. Figure S3. The relative change of NUEINST (a–f) of the six studied species induced by N addition and drought. Data are shown as mean ± SE. Among them, panel a and d show the changes in grasses, panel b and e show the changes in the leguminous forb, panel c and f show the changes in non-leguminous forbs. “N–C” and “ND–D” represent the relative changes caused by N addition without and with drought, respectively; “D–C” and “ND–N” represent the relative changes caused by drought without and with N addition, respectively. ^0.05 &lt; p &lt; 0.1, *0.01 &lt; p &lt; 0.05, **0.001 &lt; p &lt; 0.01, ***p &lt; 0.001. Figure S4. Relationships between NUEINST and WUEINST (a), and between NUELT and WUELT (b) for the six studied species under natural conditions. Figure S5. Mean net photosynthetic rate (Pn, a–c), stomatal conductance (Gs, d–f), transpiration rate (Tr, g–i), intercellular carbon dioxide concentration (Ci, j–l), leaf nitrogen content (LNC, m–o) and plant height (p–r) of the six studied species under different treatments (C: control, N: N addition, D: drought, ND: combined drought and N addition). Data are shown as mean ± SE. Among them, panels a, d, g, j, m and p show the changes in grasses, panels b, e, h, k, n and q show the changes in the leguminous forb, panels c, f, i, l o and r show the changes in non-leguminous forbs. Different lowercase letters above any two bars indicate significant differences among the treatments at p &lt; 0.05. Table S1. Information of the six studied species common to experimental plots.