Trichoderma viridulum W. T. Qin & W. Y. Zhuang HMAS 2017, sp. nov.
Creators: name: Qin, Wen-Tao
name: Zhuang, Wen-Ying
Publisher: name: Zenodo
Year: 2017
Subjects: Biodiversity
Taxonomy
Fungi
Ascomycota
Sordariomycetes
Hypocreales
Hypocreaceae
Trichoderma
Trichoderma viridulum

description: Trichoderma viridulum W.T. Qin & W.Y. Zhuang, sp. nov. FIGURES 8   
 Etymology:— The specific epithet refers to the pale green colony.   
 MycoBank: MB 816635   
 Type: — CHINA. HUBEI: Shennongjia, Huangbaoping, 1750 m, on rotten twigs, 16 Sep 2014, W. T. Qin, K. Chen, Z.Q. Zeng & H.D. Zheng 9767 (holotype HMAS 273865).   
On CMD after 72 h 50–52 mm and mycelium covering the plate after 4 d at 25 C. Colony circular, faintly zonate, aerial hyphae abundant and radially disposed. Conidiation noted after 2 d, starting at the margin of the colony, spreading to tufts or pustules, first white, gradually turning green. No distinct odor; no diffusing pigment observed.  
On PDA after 72 h 55–57 mm and mycelium covering the plate after 4 d at 25 C. Colony typically zonate, aerial hyphae abundant and densely arranged. Conidiation noted after 2 d, mainly on the concentric rings, light green. No distinct odor; no diffusing pigment observed.  
On SNA after 72 h 32–33 mm and mycelium covering the plate after 5 d at 25 C. Colony hyaline, circular, aerial hyphae sparsely disposed. Conidiation noted after 2 d, starting in short minute shrubs, spreading, developing tufts or pustules, first white, turning green gradually. Conidiophores trichoderma-like, phialides solitary or in whorls of 3–4(– 5), emerging from the main axes. Phialides lageniform to subulate, 6–12(–14) × 2.5–3 μm, l/w (2.1–)2.7–4.8(–5.6), 1.2–2.3 μm wide at the base (n = 120). Conidia green, ellipsoidal, smooth, 3–4.5(–5.2) × 2.3–3.2 μm, l/ w 1.0 –1.6 (n = 120). No chlamydospores observed. No distinct odor; no diffusing pigment observed.  
 Other specimens examined: CHINA. HEILONGJIANG: Wudalianchi, Laoheishan, 450 m, on rotten wood, 22 Aug 2014, W. T. Qin, Z.Q. Zeng & H.D. Zheng 9026, HMAS 273867; HENAN, Luoyang, Longyuwan, 1500 m, on rotten wood, 17 Sep 2013, H.D. Zheng, Z.Q. Zeng & Z.X. Zhu 8707b, HMAS 248772; HUBEI: Shennongjia, Huangbaoping, 1750 m, on twigs, 16 Sep 2014, Z.Q. Zeng, K. Chen, W. T. Qin & H.D. Zheng 9764, HMAS 273866; ibid., W. T. Qin, K. Chen, Z.Q. Zeng & H.D. Zheng 10068, HMAS 248771.   
 Notes:— Trichoderma viridulum is closely related to T. cerinum and T. tomentosum (Figure 1). The latter two fungi have pachybasium-like rather than trichoderma-like conidiophores. Trichoderma cerinum produces smaller ampulliform phialides [4.8–8.2 × 2.8–4.8 μm], smaller conidia [2.4–3.5 × 2.0–2.5 μm], obvious undulate sterile hyphal extensions, and grayish orange pigment on PDA, none of which are observed in T. viridulum (Bissett et al. 2003). Trichoderma tomentosum is peculiar for the development of green hemisphaerical pustules in culture, and in having smaller phialides [3.5–5 × 2.5–3.5 μm] and conidia [2.5–3.4 × 1.9–2.3 μm] (Bissett 1991).
description: Published as part of Qin, Wen-Tao & Zhuang, Wen-Ying, 2017, Seven new species of Trichoderma (Hypocreales) in the Harzianum and Strictipile clades, pp. 121-139 in Phytotaxa 305 (3) on pages 134-136, DOI: 10.11646/phytotaxa.305.3.1, http://zenodo.org/record/13694923