title: ÐÐ½Ð°Ð»Ð¸Ð· Ð¿Ð¾Ð´Ð²Ð¸Ð¶Ð½Ð¾ÑÑÐ¸ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð»Ð¸Ð½Ð¸Ð¹ MSCWJ-1 Ð¸ DF2 Ð² Ð¿ÑÐ¾ÑÐµÑÑÐµ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Analysis of motility of MSCWJ-1 and DF2 cell lines during replicative senescence
Creators: name: ÐÐ¾Ð»ÑÐ½ÑÐºÐ°Ñ, ÐÐ½Ð°ÑÑÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: ÐÐ¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð°Ñ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ñ
Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÐµ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸
ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ðµ ÑÑÐ°ÑÐµÐ½Ð¸Ðµ
ÐºÐ»ÐµÑÐ¾ÑÐ½Ð°Ñ Ð¿Ð¾Ð´Ð²Ð¸Ð¶Ð½Ð¾ÑÑÑ
ÐÐ¢Ð¤Ð°Ð·Ð° RhoA
Ð»Ð¸Ð·Ð¾ÑÐ¾ÑÑÐ°ÑÐ¸Ð´Ð½Ð°Ñ ÐºÐ¸ÑÐ»Ð¾ÑÐ°

description: Ð Ð°Ð±Ð¾ÑÐ° Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð° Ð½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹ Ð°ÐºÑÐ¸Ð½Ð¾Ð²Ð¾Ð³Ð¾ ÑÐ¸ÑÐ¾ÑÐºÐµÐ»ÐµÑÐ° Ð¸ Ð¿Ð¾Ð´Ð²Ð¸Ð¶Ð½Ð¾ÑÑÐ¸ Ð¼ÐµÐ·ÐµÐ½ÑÐ¸Ð¼Ð½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° (ÐÐ¡Ð) Ð² Ð¿ÑÐ¾ÑÐµÑÑÐµ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ. ÐÐ·Ð²ÐµÑÑÐ½Ð¾, ÑÑÐ¾ Ð½Ð°ÑÑÑÐµÐ½Ð¸Ñ Ð¼Ð¸Ð³ÑÐ°ÑÐ¸Ð¾Ð½Ð½ÑÑ Ð¿ÑÐ¾ÑÐµÑÑÐ¾Ð² ÑÐ¿Ð¾ÑÐ¾Ð±ÑÑÐ²ÑÑÑ ÑÑÑÐ´ÑÐµÐ½Ð¸Ñ ÑÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸Ð¸ ÑÐºÐ°Ð½ÐµÐ¹. Ð¡Ð»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾, Ð´Ð»Ñ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ñ ÐÐ¡Ð Ð² Ð²Ð¾ÑÑÑÐ°Ð½Ð¾Ð²Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð¹ Ð¼ÐµÐ´Ð¸ÑÐ¸Ð½Ðµ Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼ÑÐ¼ Ð¿ÑÐµÐ´Ð²Ð°ÑÐ¸ÑÐµÐ»ÑÐ½ÑÐ¼ ÑÑÐ»Ð¾Ð²Ð¸ÐµÐ¼ ÑÐ²Ð»ÑÐµÑÑÑ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ° ÑÐ²Ð¾Ð¹ÑÑÐ² ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ Ð»Ð¸Ð½Ð¸Ð¸, ÑÐ²ÑÐ·Ð°Ð½Ð½ÑÑ Ñ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½ÑÐ¼ ÑÑÐ°ÑÐµÐ½Ð¸ÐµÐ¼. ÐÑÐ½Ð¾Ð²Ð½Ð¾Ð¹ ÑÐµÐ»ÑÑ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð´ÐµÐ¹ÑÑÐ¸Ð²Ð¸Ñ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾ÑÐ° Ð¸ Ð°ÐºÑÐ¸Ð²Ð°ÑÐ¾ÑÐ° Rho/ROCK ÑÐ¸Ð³Ð½Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð¿ÑÑÐ¸ Ð½Ð° Ð¿Ð¾Ð´Ð²Ð¸Ð¶Ð½Ð¾ÑÑÑ ÐºÐ»ÐµÑÐ¾Ðº, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ ÑÐ´ÐµÑÐ½Ð¾-ÑÐ¸ÑÐ¾Ð¿Ð»Ð°Ð·Ð¼Ð°ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¿ÐµÑÐµÑÐ°ÑÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð±ÐµÐ»ÐºÐ° RhoA Ð² Ð¿ÑÐ¾ÑÐµÑÑÐµ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ Ð² Ð»Ð¸Ð½Ð¸ÑÑ ÐÐ¡Ð, Ð½ÐµÐ´Ð°Ð²Ð½Ð¾ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð¸ Ð¾ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸Ð·Ð¾Ð²Ð°Ð½Ð½ÑÑ Ð² Ð½Ð°ÑÐµÐ¹ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ (MSCWJ-1 Ð¸ DF2). Ð ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¸ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¾, ÑÑÐ¾ 1) ÐºÐ»ÐµÑÐºÐ¸ DF2, Ð½Ð°ÑÐ¾Ð´ÑÑÐ¸ÐµÑÑ Ð½Ð° ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ ÑÑÐ°Ð´Ð¸ÑÑ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ, Ð¾ÑÐ»Ð¸ÑÐ°ÑÑÑÑ Ð¿Ð¾ ÑÑÐ²ÑÑÐ²Ð¸ÑÐµÐ»ÑÐ½Ð¾ÑÑÐ¸ Ðº Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ð²ÐµÑÐµÑÑÐ² LPA Ð¸ Y-27632, ÑÑÐ¾ Ð·Ð°Ð¼ÐµÑÐ½Ð¾ Ð¿Ð¾ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ ÑÐºÐ¾ÑÐ¾ÑÑÐ¸ Ð¸ ÑÐ°ÑÐ°ÐºÑÐµÑÐ° ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ Ð´Ð²Ð¸Ð¶ÐµÐ½Ð¸Ñ; 2) Ð² Ð¿ÑÐ¾ÑÐµÑÑÐµ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ ÐºÐ»ÐµÑÐ¾Ðº MSCWJ-1 Ð¿ÑÐ¾Ð¸ÑÑÐ¾Ð´ÑÑ ÐºÐ¾Ð»ÐµÐ±Ð°Ð½Ð¸Ñ ÑÑÐµÐ´Ð½ÐµÐ¹ ÑÐºÐ¾ÑÐ¾ÑÑÐ¸ Ð¸ Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð½Ð¾ÑÑÐ¸ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ Ð´Ð²Ð¸Ð¶ÐµÐ½Ð¸Ñ, Ð¿ÑÐ¸ ÑÑÐ¾Ð¼ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð½ÑÐ¼ Ð´Ð²Ð¸Ð¶ÐµÐ½Ð¸ÐµÐ¼ Ð¾Ð±Ð»Ð°Ð´Ð°ÑÑ ÑÑÐ°ÑÑÐµ ÐºÐ»ÐµÑÐºÐ¸; 3) ÑÑÐ°Ð²Ð½Ð¸ÑÐµÐ»ÑÐ½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð· Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ RhoA Ð½Ð° Ð´Ð²ÑÑ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð»Ð¸Ð½Ð¸ÑÑ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð», ÑÑÐ¾ Ð² Ð¿ÑÐ¾ÑÐµÑÑÐµ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ Ð½Ð°Ð±Ð»ÑÐ´ÐµÑÑÑ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ðµ ÑÐ´ÐµÑÐ½Ð¾Ð¹ Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ RhoA.
description: The work is aimed at studying changes in the actin cytoskeleton and the motility of human mesenchymal stem cells (MSC) in the process of replicative aging. It is known that disturbance of migration processes contributes to the failure of tissue regeneration. Therefore, the characterization of the properties of the cell line associated with replicative senescence is a prerequisite for for the effective use of MSCs in restorative medicine. The main purpose of this work was to study the effects of the inhibitor and activator of the Rho / ROCK signaling pathway on cell motility, as well as to study the nuclear-cytoplasmic redistribution of the RhoA protein during replicative senescence in MSC lines recently obtained and characterized in the laboratory (MSCWJ-1 and DF2). In the course of the study, it was found that 1) DF2 cells at different stages of replicative aging differed by sensitivity to the action of LPA and Y-27632, which was noticeable by a change in the speed and type of cell movement; 2) in the process of replicative aging of MSCWJ-1 cells, fluctuations in the average speed and direction of cell movement occurred; 3) a comparative analysis of the intracellular localization of RhoA on two cell lines showed that a decrease of nuclear localization of RhoA was observed in the process of replicative senescence