cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Productive Replication of Human Papillomavirus 31 Requires DNA Repair Factor Nbs1
Creators: name: Anacker, D. C.
name: Gautam, D.
name: Gillespie, K. A.
name: Moody, C. A.
name: Chappell, W. H.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2014

description: Activation of the ATM (ataxia telangiectasia-mutated kinase)-dependent DNA damage response (DDR) is necessary for productive replication of human papillomavirus 31 (HPV31). We previously found that DNA repair and homologous recombination (HR) factors localize to sites of HPV replication, suggesting that ATM activity is required to recruit factors to viral genomes that can productively replicate viral DNA in a recombination-dependent manner. The Mre11-Rad50-Nbs1 (MRN) complex is an essential component of the DDR that is necessary for ATM-mediated HR repair and localizes to HPV DNA foci. In this study, we demonstrate that the HPV E7 protein is sufficient to increase levels of the MRN complex and also interacts with MRN components. We have found that Nbs1 depletion blocks productive viral replication and results in decreased localization of Mre11, Rad50, and the principal HR factor Rad51 to HPV DNA foci upon differentiation. Nbs1 contributes to the DDR by acting as an upstream activator of ATM in response to double-strand DNA breaks (DSBs) and as a downstream effector of ATM activity in the intra-S-phase checkpoint. We have found that phosphorylation of ATM and its downstream target Chk2, as well as SMC1 (structural maintenance of chromosome 1), is maintained upon Nbs1 knockdown in differentiating cells. Given that ATM and Chk2 are required for productive replication, our results suggest that Nbs1 contributes to viral replication outside its role as an ATM activator, potentially through ensuring localization of DNA repair factors to viral genomes that are necessary for efficient productive replication.
Behavioral Modelling of Public Transportation Passengers Participating in Last-Mile Freight Delivery
Creators: name: Mohri, Seyed Sina
name: Nassir, Neema
name: Thompson, Russell
name: Lavieri, Patricia
Publisher: name: Georgia Institute of Technology
Year: 2023
Subjects: Crowd-shipping
City logistics
Last-mile freight

description: Presented at the 9th International Physical Internet Conference (IPIC 2023) 13-15 June 2023, Athens, Greece
description: An emerging stream of Crowd-Shipping (CS) solutions focuses on existing momentum in Public Transportation (PT) to ship viable delivery packages by PT passengers. Few studies have explored the package delivery acceptance behavior of passengers engaged in PT-based CS initiatives while passengers’ behavioral intention to participate (i.e., engage) is not studied. It is requisite that newly introduced CS platforms explore their potential crowdshippers’ behavior on intention to participate and set efficient marketing strategies. Given a survey data collected from 2208 PT passengers in Sydney metropolitan area, this study explores the intention of PT passengers as crowd-shippers to participate in PT-based CS initiatives, as well as prohibiting factors in way of participation.
Finzioni che non funzionano: due corpi di troppo
Creators: name: Nanni, Peppe
Publisher: name: La Rivista di Engramma
Year: 2023
Subjects: Mysteries of State
Monarchy
Republican thought

description: Taking the cue from the coronation ceremony of Charles III, the paper questions the reasons for the institutional persistence of the Monarchy, an archaeological remnant of the Age of Absolutism which still maneouvre the constitutions of contemporary Europe. This includes those countries that are formally Republics and on which the gloomy legacy of absolute monarchy persists with a faint authoritarian influence. In the light of Ernst Kantorovicz’s seminal books, this paper focuses on the arcana imperii, the “Mysteries of State”, which, behind the glittering glare of ceremonies and rhetorical language and complicated mechanisms designed to seduce people, conceals all the illegal actions indispensable to perpetuate systems of power and, to this date, continues to limit the freedom of a conscious political choice – even in the societies that proclaim themselves “democratic”.
Quantification of Entry Phenotypes of Macrophage-Tropic HIV-1 across a Wide Range of CD4 Densities
Creators: name: Swanstrom, A. E.
name: Lee, B.
name: Arrildt, K. T.
name: Joseph, S. B.
name: Hoxie, J. A.
name: Schnell, G.
name: Swanstrom, R.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2014

description: Defining a macrophage-tropic phenotype for HIV-1 to assess a role in pathogenesis is complicated by the fact that HIV-1 isolates vary continuously in their ability to enter monocyte-derived macrophages (MDMs) in vitro, and MDMs vary in their ability to support HIV-1 entry. To overcome these limitations, we identified consistent differences in entry phenotypes between five paired blood-derived, T cell-tropic HIV-1 env genes, four of which are CCR5-using (R5) and one of which is CXCR4-using (X4), and cerebrospinal fluid (CSF)-derived, R5 macrophage-tropic env genes. We performed entry assays using the CD4- and CCR5-inducible Affinofile cell line, expressing a range of CD4 levels that approximates the range from MDMs to CD4+ T cells. The macrophage-tropic viruses were significantly better at infecting cells expressing low levels of CD4 than the T cell-tropic viruses from the same subjects, with the titration of CD4 providing a distinctive and quantitative phenotype. This difference in CD4 utilization was not due to macrophage-tropic viruses being CD4 independent. Furthermore, macrophage-tropic viruses did not differ from paired T cell-tropic viruses in their ability to use low levels of CCR5 (tpaired = −1.39; P = 0.24) or their use of an alternative conformation of CCR5. We also infected MDMs with a panel of viruses and observed that infectivity of each virus differed across four donors and between three preparations from a single donor. We concluded that the evolutionary transition from replication in T cells to that in macrophages involves a phenotypic transition to acquire the ability to infect cells expressing low levels of CD4 and that this phenotype is more reliably measured in Affinofile cells than in macrophages.
UniKW-AT: Unified Keyword Spotting and Audio Tagging
Creators: name: Dinkel, Heinrich
name: Wang, Yongqing
name: Yan, Zhiyong
name: Zhang, Junbo
name: Wang, Yujun
Publisher: name: arXiv
Year: 2022
Subjects: subject: Sound (cs.SD)
subject: Audio and Speech Processing (eess.AS)
subject: FOS: Computer and information sciences
subject: FOS: Electrical engineering, electronic engineering, information engineering

description: Within the audio research community and the industry, keyword spotting (KWS) and audio tagging (AT) are seen as two distinct tasks and research fields. However, from a technical point of view, both of these tasks are identical: they predict a label (keyword in KWS, sound event in AT) for some fixed-sized input audio segment. This work proposes UniKW-AT: An initial approach for jointly training both KWS and AT. UniKW-AT enhances the noise-robustness for KWS, while also being able to predict specific sound events and enabling conditional wake-ups on sound events. Our approach extends the AT pipeline with additional labels describing the presence of a keyword. Experiments are conducted on the Google Speech Commands V1 (GSCV1) and the balanced Audioset (AS) datasets. The proposed MobileNetV2 model achieves an accuracy of 97.53% on the GSCV1 dataset and an mAP of 33.4 on the AS evaluation set. Further, we show that significant noise-robustness gains can be observed on a real-world KWS dataset, greatly outperforming standard KWS approaches. Our study shows that KWS and AT can be merged into a single framework without significant performance degradation.
description: Accepted in Interspeech2022
Performance of Self Organizing Logistics: a Practical Comparison between Centralized and Decentralized Logistics
Creators: name: Fransen, Ruben
name: Sprenger, Lola
name: Lee, Wan-Jui
name: Van Meijeren, Jaco
Publisher: name: Georgia Institute of Technology
Year: 2023
Subjects: Autonomous transport
Centralized control
Container logistics
Self-organizing logistics (SOL)
Supply chain management

description: Presented at the 9th International Physical Internet Conference (IPIC 2023) 13-15 June 2023, Athens, Greece
description: In the vision of Physical Internet there is no central authority that regulates decision making and asset use, the decision making is decentralized to make logistics self-organizing. A theoretical downside of decentralization is reduction of system performance due to lack of system overview leading to sub-optimal decisions. This research shows that the system performance is not significantly reduced when making decentralized decisions in a trucking network on small problem instances. Furthermore, the theoretical advantages of decentralized control compared to centralized are evaluated with the practical implementation.
Quantitation of Replication-Competent HIV-1 in Populations of Resting CD4+ T Cells
Creators: name: Crooks, A. M.
name: Margolis, D. M.
name: Archin, N. M.
name: Bateson, R. E.
name: Dahl, N. P.
name: Soriano-Sarabia, N.
name: Kuruc, J. D.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2014

description: Central memory (TCM) CD4+ T cells are the principal reservoir of latent HIV-1 infection that persists despite durable, successful antiretroviral therapy (ART). In a study that measured HIV DNA in 17 patients and replication-competent HIV in 4 patients, pools of resting and activated transitional memory (TTM) CD4+ T cells were found to be a reservoir for HIV infection. As defective viruses account for the majority of integrated HIV DNA and do not reflect the actual frequency of latent, replication-competent proviral infection, we assessed the specific contribution of resting TTM cells to latent HIV infection. We measured the frequency of replication-competent HIV in purified resting memory cell subpopulations by a limiting-dilution, quantitative viral outgrowth assay (QVOA). HIV was routinely detected within the resting central memory compartment but was infrequently detected within the resting TTM compartment. These observations suggest that prolonged ART may limit persistent latent infection in the TTM compartment. Our results confirm the importance of latent infection within the TCM compartment and again focus attention on these cells as the most important latent viral reservoir. While proliferation may drive expansion of detectable viral genomes in cells, the frequency of replication-competent HIV must be carefully assessed. Latent infection appears to wane within the transitional memory compartment in patients who have sustained successful viral suppression via ART or were treated very early in infection.
Learning by Doing: Introducing Students to Public History through Digital Projects
Creators: name: Lucchesi, Anita
name: Legay, Richard
Publisher: name: A. Lucchesi, R. Legay, Learning by Doing: Introducing Students to Public History through Digital Projects, «International Public History», 2, 2019, n. 1, pp. 1-3
Year: 2019
Subjects: Public history
Digital public history
Teaching
Experimentation

description: This article reflects on the process of building and teaching a course in digital public history for Bachelor students at the University of Luxembourg. The heart of the program was the hands-on, experimental approach. The article describes the organization of the course and presents some of the strategies followed to lead the students through a more holistic and playful approach. The experience shows that the learning by doing motto can be an effective path for teaching public history and especially digital public history, as the digital element opens space for more experimentation.
On the thermodynamic foundations of plasticity
Creators: name: Péter, Ván
Publisher: name: arXiv
Year: 2014
Subjects: subject: Materials Science (cond-mat.mtrl-sci)
subject: FOS: Physical sciences

description: In this work the relation of plastic and rheological material models is analysed in the framework of non-equilibrium thermodynamics. After a short summary of the basic notions of classical elasticity and plasticity the traditional thermodynamic treatment of plasticity by Ziegler is introduced, where the plastic deformation is an internal variable, leading to the reduction of thermostatic stress. Then a simple thermodynamic treatment of static and dynamic friction follows, because the continuum generalization of these phenomena leads to viscosity and plasticity in our interpretation. Finally, plasticity is treated in the framework of non-equilibrium thermodynamics. The yield condition is the consequence of the dependence of the Onsagerian conductivity coefficients on the absolute value of the strain rate. Our treatment unifies rheology and plasticity in a common framework. --- Ebben az irasban a keplekeny es a reologiai anyagmodellek kapcsolatat targyaljuk a nemegyensulyi termodinamika alapjan. A klasszikus rugalmassagtan es keplekenysegtan alapfogalmainak rovid osszefoglalasa utan reszletesen vizsgaljuk a Ziegler-tol eredo termodinamikai keplekenysegelmeletet, amelyben a keplekeny deformacio a termosztatikai feszultseget csokkento belso valtozo. Ezutan a csuszasi es tapadasi surlodas egyszeru modelljet targyaljuk, mert esetunkben ezeknek a kontinuumokra torteno altalanositasa vezet a viszkozitashoz, illetve a keplekenyseghez. A keplekenysegi feltetelt az Onsager-fele vezetesi egyutthatoknak a belso valtozo sebessegenek abszolut erteketol valo fuggese hordozza. A keplekeny es a rugalmas deformacio idobeli valtozasat reologiai hatasokkal egyutt targyaljuk. (arXiv egyelore nem tud ekezeteket a kivonatban.)
description: 28 pages, 9 figures, in Hungarian
Additional file 1 of Patient engagement as a collaborative process in a large Dutch COVID-19 vaccination study (RECOVAC) – insight into the contribution of patient engagement and learnings for the future
Creators: name: Vervoort, J. P. M.
name: Konijn, W. S.
name: Jansen, D. E. M. C.
name: Boersma, C.
name: de Zeeuw, J.
name: Ho-dac – Pannekeet, M. M.
name: Gansevoort, R. T.
name: Messchendorp, A. L.
name: Sanders, J. S. F.
name: de Wildt-Liesveld, R.
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Immunology
subject: FOS: Clinical medicine
subject: Cancer
subject: Science Policy
subject: Mental Health
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Virology

description: Supplementary Material 1
MAKTABGACHA TAʼLIM TASHKILOTLARI TARBIYACHILARINING AXBOROT KOMPETENTLIGINI RIVOJLANTIRISHNING NAZARIY ASOSLARI
Creators: name: Mavlonov, N.Sh.
Publisher: name: Zenodo
Year: 2023

description: Yurtimiz taʼlim tizimining zamonaviy rivojlanish ehtiyojlaridan biri  maktabgacha taʼlim tizimini axborotlashtirish va raqamlashtirishdir. Axborot-kommynikatsiya texnologiyalarining (AKT) didaktik imkoniyatlarini rivojlantirish va ularni taʼlimga joriy etish, pecpyblikada Internetga ulanishning tobora kengayib borishi, onlayn oʻqitish tizimi, masofaviy oʻqitish tizimiga oʻtish maktabgacha taʼlimi tashkilotlari pedagogining informatsion kommynikativ kompetentligini rivojlantirishga olib keladi.
Closing the information gap of multimodal transports
Creators: name: Zajicek, Juergen
name: Brandstätter, Georg
Publisher: name: Georgia Institute of Technology
Year: 2023
Subjects: Interconnected freight transport

description: Presented at the 9th International Physical Internet Conference (IPIC 2023) 13-15 June 2023, Athens, Greece
description: Customer and freight related information is essentially to enable and ensure reliable transport chains. Therefore, real-time information along the transport service is to be made available by the participating companies to enable each service provider to optimise its resources and dispositive control of its processes. This Paper deals with the result of the project RRTM-C which tested a closed information chain of an exemplary daily multimodal container shuttle service running in Austria between Vienna to Bludenz. The used information and data were provided by combining the tools that the project partners OeBB (Austrian Federal Railways), Asfinag (Austrian Motorway and Expressway Financing Joint-Stock Company) and shipper company Venz Logistik use for their daily operation. By connecting the main tools of the partners via specialised interfaces a continuous, cross- modal exchange of information including a stable prediction of ETA (Estimated Time of Arrival) of the containers in the terminals was made possible. The system was tested successfully in real transport conditions in June and September 2022. Additionally, an optimisation model of the involved container terminal was implemented to research the effects of different scenarios of different grades of information availability in the container terminal. RRTM-C was a national cooperative implementation project that that was funded by the Austrian Ministry of Climate Action, Environment, Energy, Mobility, Innovation and Technology (BMK) and the National Railway Infrastructure Funding Agency (SCHIG).
Local Diamagnetic Susceptibility of Quasi-Two-Dimensional Graphite
Creators: name: Nikolaev, E. G.
name: Kotosonov, A. S.
name: Shalashugina, E. A.
name: Troyanovskii, A. M.
name: Tsebro, and V. I.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Materials Science (cond-mat.mtrl-sci)
subject: FOS: Physical sciences

description: A sample of quasi-two-dimensional graphite (QTDG) whose magnetic properties are described within the Dirac fermion model is investigated by the nuclear magnetic resonance (NMR) and scanning tunneling microscopy (STM) techniques. The broad spectrum of the sample points to a large dispersion of crystallite sizes in this system, which is also confirmed by STM data. It is established that the local diamagnetic susceptibility may substantially exceed the average value over the sample and reaches an abnormally high value of $-1.3\times 10^{-4}$ emu/g at $T = 4.2$ K, which is greater than the corresponding value of highly oriented graphite by a factor of four.
description: 9 pages, 3 figures
Recombinant Adeno-Associated Virus Utilizes Cell-Specific Infectious Entry Mechanisms
Creators: name: Nicolson, S.
name: Samulski, R. J.
name: Weinberg, M. S.
name: McLendon, M.
name: Li, C.
name: Bhatt, A. P.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2014

description: Understanding the entry and trafficking mechanism(s) of recombinant adeno-associated virus (rAAV) into host cells can lead to evolution in capsid and vector design and delivery methods, resulting in enhanced transduction and therapeutic gene expression. Variability of findings regarding the early entry pathway of rAAV supports the possibility that rAAV, like other viruses, can utilize more than one infectious entry pathway. We tested whether inhibition of macropinocytosis impacted rAAV transduction of HeLa cells compared to hepatocellular carcinoma cell lines. We found that macropinocytosis inhibitor cytochalasin D blocked rAAV transduction of HeLa cells (>2-fold) but enhanced (10-fold) transduction in HepG2 and Huh7 lines. Similar results were obtained with another macropinocytosis inhibitor, 5-(N-ethyl-N-isopropyl) amiloride (EIPA). The augmented transduction was due to neither viral binding nor promoter activity, affected multiple rAAV serotypes (rAAV2, rAAV2-R585E, and rAAV8), and influenced single-stranded and self-complementary virions to comparable extents. Follow-up studies using CDC42 inhibitor ML141 and p21-activated kinase 1 (PAK1) siRNA knockdown also resulted in enhanced HepG2 transduction. Microscopy revealed that macropinocytosis inhibition correlated with expedited nuclear entry of the rAAV virions into HepG2 cells. Enhancement of hepatocellular rAAV transduction extended to the mouse liver in vivo (4-fold enhancement) but inversely blocked heart tissue transduction (13-fold). This evidence of host cell-specific rAAV entry pathways confers a potent means for controlling and enhancing vector delivery and could help unify the divergent accounts of rAAV cellular entry mechanisms.
Recombinant Adeno-Associated Virus Utilizes Host Cell Nuclear Import Machinery To Enter the Nucleus
Creators: name: Nicolson, S. C.
name: Samulski, R. J.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2014

description: Recombinant adeno-associated viral (rAAV) vectors have garnered much promise in gene therapy applications. However, widespread clinical use has been limited by transduction efficiency. Previous studies suggested that the majority of rAAV accumulates in the perinuclear region of cells, presumably unable to traffic into the nucleus. rAAV nuclear translocation remains ill-defined; therefore, we performed microscopy, genetic, and biochemical analyses in vitro in order to understand this mechanism. Lectin blockade of the nuclear pore complex (NPC) resulted in inhibition of nuclear rAAV2. Visualization of fluorescently labeled particles revealed that rAAV2 localized to importin-β-dense regions of cells in late trafficking steps. Additionally, small interfering RNA (siRNA) knockdown of importin-β partially inhibited rAAV2 nuclear translocation and inhibited transduction by 50 to 70%. Furthermore, coimmunopreciptation (co-IP) analysis revealed that capsid proteins from rAAV2 could interact with importin-β and that this interaction was sensitive to the small GTPase Ran. More importantly, mutations to key basic regions in the rAAV2 capsid severely inhibited interactions with importin-β. We tested several other serotypes and found that the extent of importin-β interaction varied, suggesting that different serotypes may utilize alternative import proteins for nuclear translocation. Co-IP and siRNA analyses were used to investigate the role of other karyopherins, and the results suggested that rAAV2 may utilize multiple import proteins for nuclear entry. Taken together, our results suggest that rAAV2 interacts with importin-β alone or in complex with other karyopherins and enters the nucleus via the NPC. These results may lend insight into the design of novel AAV vectors that have an enhanced nuclear entry capability and transduction potential.
Ferroelectricity controlled chiral spin textures and anomalous valley Hall effect in the Janus magnet-based multiferroic heterostructure
Creators: name: Zhu, Yingmei
name: Cui, Qirui
name: Liang, Jinghua
name: Ga, Yonglong
name: Yang, Hongxin
Publisher: name: arXiv
Year: 2022
Subjects: subject: Mesoscale and Nanoscale Physics (cond-mat.mes-hall)
subject: Materials Science (cond-mat.mtrl-sci)
subject: FOS: Physical sciences

description: Realizing effective manipulation and explicit identification of topological spin textures are two crucial ingredients to make them as information carrier in spintronic devices with high storage density, high data handling speed and low energy consumption. Electric-field manipulation of magnetism has been achieved as a dissipationless method compared with traditional regulations. However, the magnetization is normally insensitive to the electric field since it does not break time-reversal symmetry directly, and distribution of topological magnetic quasiparticles is difficult to maintain due to the drift arising from external fluctuation, which could result in ambiguous recognition between quasiparticles and uniform magnetic background. Here, we demonstrate that electric polarization-driven skyrmionic and uniform ferromagnetic states can be easily and explicitly distinguished by transverse voltage arising from anomalous valley Hall effect in the Janus magnet-based multiferroic heterostructure LaClBr/In2Se3. Our work provides an alternative approach for data encoding, in which data are encoded by combing topological spin textures with detectable electronic transport.
description: published in 2D materials, 9, 045030 (2022)
Additional file 1 of Awareness and use of home remedies in Italy’s alps: a population-based cross-sectional telephone survey
Creators: name: Wiedermann, Wolfgang
name: Ausserhofer, Dietmar
name: Vögele, Anna
name: Becker, Ulrich
name: Piccoliori, Giuliano
name: Wiedermann, Christian J.
name: Engl, Adolf
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Cell Biology
subject: Ecology
subject: FOS: Biological sciences
subject: Sociology
subject: FOS: Sociology
subject: Science Policy

description: Supplementary Material 1
Dzyaloshinskii-Moriya interaction and magnetic skyrmions induced by curvature
Creators: name: Ga, Yonglong
name: Cui, Qirui
name: Liang, Jinghua
name: Yu, Dongxing
name: Zhu, Yingmei
name: Wang, Liming
name: Yang, Hongxin
Publisher: name: arXiv
Year: 2022
Subjects: subject: Materials Science (cond-mat.mtrl-sci)
subject: FOS: Physical sciences

description: Realizing sizeable Dzyaloshinskii-Moriya interaction (DMI) in intrinsic two-dimensional (2D) magnets without any manipulation will greatly enrich potential application of spintronics devices. The simplest and most desirable situation should be 2D magnets with intrinsic DMI and intrinsic chiral spin textures. Here, we propose to realize DMI by designing periodic ripple structures with different curvatures in low-dimensional magnets and demonstrate the concept in both one-dimensional (1D) CrBr2 and two-dimensional (2D) MnSe2 magnets by using first-principles calculations. We find that DMIs in curved CrBr2 and MnSe2 can be efficiently controlled by varying the size of curvature c, where c is defined as the ratio between the height h and the length l of curved structure. Moreover, we unveil that the dependence of first-principles calculated DMI on size of curvature c can be well described by the three-site Fert-Lévy model. At last, we uncover that field-free magnetic skyrmions can be realized in curved MnSe2 by using atomistic spin model simulations based on first-principles calculated magnetic parameters. The work will open a new avenue for inducing DMI and chiral spin textures in simple 2D magnets via curvature.
description: Published on Physical Review B 106, 054426
Replication of Oral BK Virus in Human Salivary Gland Cells
Creators: name: Gingerich, A. D.
name: Hallett, R. A.
name: Madden, V.
name: Nickeleit, V.
name: Burger-Calderon, R.
name: Webster-Cyriaque, J.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2014

description: BK polyomavirus (BKPyV) is the most common viral pathogen among allograft patients. Increasing evidence links BKPyV to the human oral compartment and to HIV-associated salivary gland disease (HIVSGD). To date, few studies have analyzed orally derived BKPyV. This study aimed to characterize BKPyV isolated from throat wash (TW) samples from HIVSGD patients. The replication potential of HIVSGD-derived clinical isolates HIVSGD-1 and HIVSGD-2, both containing the noncoding control region (NCCR) architecture OPQPQQS, were assessed and compared to urine-derived virus. The BKPyV isolates displayed significant variation in replication potential. Whole-genome alignment of the two isolates revealed three nucleotide differences that were analyzed for a potential effect on the viral life cycle. Analysis revealed a negligible difference in NCCR promoter activity despite sequence variation and emphasized the importance of functional T antigen (Tag) for efficient replication. HIVSGD-1 encoded full-length Tag, underwent productive infection in both human salivary gland cells and kidney cells, and expressed viral DNA and Tag protein. Additionally, HIVSGD-1 generated DNase-resistant particles and by far surpassed the replication potential of the kidney-derived isolate in HSG cells. HIVSGD-2 encoded a truncated form of Tag and replicated much less efficiently. Quantitation of infectious virus, via the fluorescent forming unit assay, suggested that HIVSGD BKPyV had preferential tropism for salivary gland cells over kidney cells. Similarly, the results suggested that kidney-derived virus had preferential tropism for kidney cells over salivary gland cells. Evidence of HIVSGD-derived BKPyV oral tropism and adept viral replication in human salivary gland cells corroborated the potential link between HIVSGD pathogenesis and BKPyV.
Digital Twinning platforms as an enabler for the ex-ante evaluation of PI-inspired interventions to last-mile logistics networks
Creators: name: Rizopoulos, Dimitrios
name: Niavis, Harris
name: Kampa, Maria
name: Zafeiropoulou, Aristea M.
name: Franklin, Rod
name: Mygiakis, Antonis
name: Kakouris, Anastasios
name: Alexopoulos, Andreas
name: Fergadiotou, Ioanna
Publisher: name: Georgia Institute of Technology
Year: 2023
Subjects: Digital twins
PaaS
Simulation
Blockchain
Trusted data sharing

description: Presented at the 9th International Physical Internet Conference (IPIC 2023) 13-15 June 2023, Athens, Greece
description: One of the greatest challenges of the European last mile logistics sector is the requirement to decouple its economic growth from resource use and air pollutants emissions from transport operations. This requirement, alongside the rise of e-commerce and the phenomenon of urbanization, creates an urgent need for European Union's member states to identify and rapidly upscale innovative last-mile solutions that will ensure the green and digital transformation of European urban environments. To achieve such a goal, innovative frameworks such as the Physical Internet (PI) are required, which can lead to low-emission logistics services that remain competitive compared to the latest industry trends such as sameor next-day-delivery, real-time parcel tracking, and omni-channel distribution. The URBANE project introduces an Innovation Transferability Platform with Digital Twinning capabilities, under the Platform-as-a-service (PaaS) paradigm, which enables the project's Living Labs to assess the impact of the PI-inspired interventions before or during their implementation in the real-world context. In that regard, in the current article, a presentation of initial project results is given, including platform micro-services and Use Cases (UCs) that aim at supporting the transition to the PI.
Residue 82 of the Chikungunya Virus E2 Attachment Protein Modulates Viral Dissemination and Arthritis in Mice
Creators: name: Ashbrook, A. W.
name: Morrison, T. E.
name: Burrack, K. S.
name: Dermody, T. S.
name: Montgomery, S. A.
name: Silva, L. A.
name: Heise, M. T.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2014

description: Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that has reemerged to cause profound epidemics of fever, rash, and arthralgia throughout sub-Saharan Africa, Southeast Asia, and the Caribbean. Like other arthritogenic alphaviruses, mechanisms of CHIKV pathogenesis are not well defined. Using the attenuated CHIKV strain 181/25 and virulent strain AF15561, we identified a residue in the E2 viral attachment protein that is a critical determinant of viral replication in cultured cells and pathogenesis in vivo. Viruses containing an arginine at E2 residue 82 displayed enhanced infectivity in mammalian cells but reduced infectivity in mosquito cells and diminished virulence in a mouse model of CHIKV disease. Mice inoculated with virus containing an arginine at this position exhibited reduced swelling at the site of inoculation with a concomitant decrease in the severity of necrosis in joint-associated tissues. Viruses containing a glycine at E2 residue 82 produced higher titers in the spleen and serum at early times postinfection. Using wild-type and glycosaminoglycan (GAG)-deficient Chinese hamster ovary (CHO) cell lines and soluble GAGs, we found that an arginine at residue 82 conferred greater dependence on GAGs for infection of mammalian cells. These data suggest that CHIKV E2 interactions with GAGs diminish dissemination to lymphoid tissue, establishment of viremia, and activation of inflammatory responses early in infection. Collectively, these results suggest a function for GAG utilization in regulating CHIKV tropism and host responses that contribute to arthritis.
Osteitis pubis: can early return to elite competition be contemplated?
Creators: name: (:Unkn) Unknown
Publisher: name: Universita' degli Studi di Salerno
Year: 2014
Subjects: Osteitis pubis
Rehabilitation
Lumbo-pelvic stabilization
Sport

description: Background and purpose In elite athletes, osteitis pubis is a common painful degenerative process of the pubic symphysis and surrounding soft tissues and tendons. We report the diagnostic pathway and the rehabilitation protocol of six elite athletes with osteitis pubis in three different sports, and compare protocol stages and time to return to competition. Methods 6 athletes (2 soccer, 2 basketball, 2 rugby players) were diagnosed with osteitis pubis stage III and IV according to Rodriguez classification using standard clinical and imaging criteria. After performing a baseline lumbo-pelvic assessment, the rehabilitation protocol described by Verrall was adapted to each individual athlete. Results The length of time for each stage of the protocol was as follows; Stage 1 (rest from sport) was 26 +/- 5 days, Stage 2 (to achieve pain free running), 18 +/- 5 days, Stage 3 (squad training) 63 +/- 7, Stage 4 (return to competition) 86 +/- 15. Soccer players took longer to return to competition than basketball and rugby players. No recurrences were reported at 2 year follow-up. Conclusion The protocol presented ensures a safe return to elite athletes. The time from diagnosis to full recovery is longer in football players, and seems to increase with age.
The Urban Cloud: Linking city services, cloud computing, and the Physical Internet to achieve smart city objectives
Creators: name: Franklin, Rod
name: Fergadiotou, Ioanna
name: Kampa, Maria
name: Rizopoulos, Dimitris
name: Tavasszy, Lorant
Publisher: name: Georgia Institute of Technology
Year: 2023
Subjects: Urban Logistics
Last mile delivery
Infrastructure as a service
Internet of things
Physical Internet
Cloud computing
Sensors

description: Presented at the 9th International Physical Internet Conference (IPIC 2023) 13-15 June 2023, Athens, Greece
description: Urban logistics operations have become more complex and problematic for cities. The last mile delivery situation, driven by the growth in eCommerce purchases, is creating congestion and sustainability problems that cities must address if they are to meet the expectations of their citizens for a socially enjoyable and sustainable living space. This working Paper explores the potential restructuring of how cities manage their infrastructure to achieve these objectives. Building on the fact that cities have increasingly built intelligence into their infrastructures through the installation of sensors, the Paper examines the potential for reorganizing the management of this infrastructure using cloud computing three layers of Infrastructure, Platform, and Software as a Service architecture as a model.
Perfect and Stable Hybrid Glasses from Strong and Fragile Metal-Organic Framework Liquids
Creators: name: Bennett, T. D.
name: Tan, Jin-Chong
name: Yue, Y. Z.
name: Ducati, C.
name: Terril, N.
name: Yeung, H. H. M.
name: Zhou, Z.
name: Chen, W.
name: Henke, S.
name: Cheetham, A. K.
name: Greaves, G. N.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Materials Science (cond-mat.mtrl-sci)
subject: FOS: Physical sciences

description: Hybrid glasses connect emerging fields of metal-organic frameworks (MOFs) with the glass-formation, amorphization, and melting processes of these structurally diverse and chemically versatile systems. Most zeolites, including MOFs, amorphize around the glass transition, devitrifying and then melting at much higher temperatures. The relationship between the two processes has so far not been investigated. Herein we show how heating first results in a low density perfect glass, following an order-order transition, leading to a super-strong liquid of low fragility that dynamically controls MOF collapse. A subsequent order-disorder transition creates a high density liquid of greater fragility. After crystallization and melting, subsequent cooling results in a stable glass virtually identical to the high density phase. Furthermore, the wide-ranging melting temperatures of different MOFs suggest these can be differentiated by topology. Our research provides new insight into the stability and functionality of these novel ductile crystalline materials, including the possibility of melt-casting MOFs.
description: 15 pages, 4 figures
Scan4Transport: Connecting the transport unit to its digital twin
Creators: name: Voorspuij, Jaco
name: Ruighaver, Michiel
Publisher: name: Georgia Institute of Technology
Year: 2023
Subjects: Digital twin
Transport unit
Resilience
Supply chain visibility
SME inclusion
Linked data

description: Presented at the 9th International Physical Internet Conference (IPIC 2023) 13-15 June 2023, Athens, Greece
description: The transport and logistics industry has long struggled with ensuring that operators could always effectively and efficiently handle the transport units at hand. Three challenges especially hampered the industry: 1. Transport units are often handled by several operators from source to destination. Many operators are SME with low IT capabilities. 2. Connectivity to an IT system is often not feasible and thus access to remote data not possible. 3. Proprietary approaches by large LSP makes things worse for the SME. Scan4Transport1 (S4T) is a global standard for encoding transport data in a 2D barcode on a Logistics Label attached to Transport Units, supporting organisations across the entire transport chain from seller to buyer. The 2D barcode: 1. contains the minimum information available for the (field) operator even in case there is no connection with a remote host system. 2. may be read with modern standard mobile phones that all operators (including SME) will have with them. 3. is globally standardized independent from any shipper, carrier, receiver, LSP or other stakeholder. Any operator working with several/many other operators can suffice with a single application to interpret the 2D barcode and be able to handle the transport unit effectively and efficiently.
title: Selection matrix for Brazilian bee species to risk assessment of pesticides
Creators: name: Viana-Silva, Flavia
name: Pires, Carmen
name: Torezani, Karoline
name: Borges, Leandro
name: Cham, Karina
name: Dias, Christiane
name: Teixeira, Ivan
name: Tonelli, Carlos
name: Belchior, Ceres
name: Marcondes, Cayssa
name: Nocelli, Roberta
name: Malaspina, Osmar
name: Cione, Ana
name: Shiva, Andreia
name: Ferraz, Andreia
Publisher: name: Julius Kühn-Institut
Year: 2018

description: Many countries are using honeybee (Apis mellifera) as a surrogate to evaluate the risk of pesticides to all bee species. However, there is uncertainty regarding the extent honey bees can be used as surrogates for non‐Apis species in pesticides risk assessment. A selection matrix for Brazilian bee species was built to support the selection process. To be considered as a candidate representative species in the Brazilian agricultural scenario a bee should have a wide geographic distribution, and be recorded in at least 4 agricultural crops. The selection matrix provides a foundation to elect meliponines (stingless bees) as a priority group. Therefore, in the near future Ibama intends to assess the need for changes in the risk assessment procedure for bees, eventually including a stingless bee as a representative species.
description: Julius-Kühn-Archiv, Nr. 462 (2018): Hazards of pesticides to bees 13th International Symposium of the ICP-PR Bee Protection Group 18. - 20. October 2017, València (Spain) - Proceedings -
Additional file 2 of Patient engagement as a collaborative process in a large Dutch COVID-19 vaccination study (RECOVAC) – insight into the contribution of patient engagement and learnings for the future
Creators: name: Vervoort, J. P. M.
name: Konijn, W. S.
name: Jansen, D. E. M. C.
name: Boersma, C.
name: de Zeeuw, J.
name: Ho-dac – Pannekeet, M. M.
name: Gansevoort, R. T.
name: Messchendorp, A. L.
name: Sanders, J. S. F.
name: de Wildt-Liesveld, R.
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Immunology
subject: FOS: Clinical medicine
subject: Cancer
subject: Science Policy
subject: Mental Health
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Virology

description: Supplementary Material 2
Identify the topological superconducting order in a multi-band quantum wire
Creators: name: Wang, Pei
name: Liu, Jie
name: Sun, Qing-feng
name: Xie, X. C.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Mesoscale and Nanoscale Physics (cond-mat.mes-hall)
subject: Superconductivity (cond-mat.supr-con)
subject: FOS: Physical sciences

description: How to distinguish the zero-bias peak (ZBP) caused by the Majorana fermions from that by the other effects remains a challenge in detecting the topological order of a quantum wire. In this paper we propose to distinguish the topological superconducting phase from the topologically trivial phase by making a Josephson junction of the quantum wire attached to a side lead and then measuring the tunneling conductance through it as the phase difference across the junction $ϕ$ varies. Even if the ZBPs exist in both phases, we can identify the topological superconducting phase by a conductance peak at $ϕ=π$ and a nearby butterfly pattern.
description: 5 pages, 4 figures
Dynamically generated resonances from the vector meson-octet baryon interaction in the strangeness zero sector
Creators: name: Sun, Bao-Xi
name: Lu, Xiao-Fu
Publisher: name: arXiv
Year: 2014
Subjects: subject: High Energy Physics - Phenomenology (hep-ph)
subject: Nuclear Theory (nucl-th)
subject: FOS: Physical sciences

description: The interaction potentials between vector mesons and octet baryons are calculated explicitly with a summation of t-, s-, u-channel diagrams and a contact term originating from the tensor interaction. Many resonances are generated dynamically in different channels of strangeness zero by solving the coupled-channel Lippman-Schwinger equations with the method of partial wave analysis, and their total angular momenta are determined. The spin partners N(1650)1/2^{-} and N(1700)3/2^-, N(1895)1/2^{-} and N(1875)3/2^-, and the state N(2120)3/2^- are all produced respectively in the isospin I=1/2 sector. In the isospin I=3/2 sector, the spin partners Delta(1620)1/2^- and Delta(1700)3/2^- are also associated with the pole in the complex energy plane. According to the calculation results, a J^P=1/2^- state around 2000 MeV is predicted as the spin partner of N(2120)3/2^-. Some resonances are well fitted with their counterparts listed in the newest review of Particle Data Group(PDG), while others might stimulate the experimental observation in these energy regions in the future.
description: 28 pages, 12 figures, 8 tables. arXiv admin note: text overlap with arXiv:0905.0973 by other authors
Those of a Queer Age: Insights into Aging in the Gay and Lesbian Community
Creators: name: Schmitz-Bechteler, Stephanie
Publisher: name: Unknown
Year: 2006

description: Aging is not a homogeneous experience, but rather a process guided by the life experiences and life course decisions made by the individual. Each person is uniquely affected by generational and cultural cohort experiences that shape not only the contexts of her or his life, but the manner in which the individual experiences the aging process. Gay and lesbian individuals experience an aging process that is uniquely different from that of heterosexual individuals. Additionally, gays and lesbians from different generational cohorts will have disparate aging experiences. This article examines aging among different gay and lesbian generational cohorts.
RNA Populations in Immunocompromised Patients as Reservoirs for Novel Norovirus Variants
Creators: name: Donaldson, E.
name: Vega, E.
name: Chen, L. F.
name: Vinje, J.
name: Barclay, L.
name: Huynh, J.
name: Baric, R.
name: Lopman, B.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2014

description: Noroviruses are the leading cause of acute gastroenteritis outbreaks worldwide. The majority of norovirus outbreaks are caused by genogroup II.4 (GII.4). Novel GII.4 strains emerge every 2 to 4 years and replace older variants as the dominant norovirus. Novel variants emerge through a combination of recombination, genetic drift, and selection driven by population immunity, but the exact mechanism of how or where is not known. We detected two previously unknown novel GII.4 variants, termed GII.4 UNK1 and GII.4 UNK2, and a diverse norovirus population in fecal specimens from immunocompromised individuals with diarrhea after they had undergone bone marrow transplantation. We hypothesized that immunocompromised individuals can serve as reservoirs for novel norovirus variants. To test our hypothesis, metagenomic analysis of viral RNA populations was combined with a full-genome bioinformatic analysis of publicly available GII.4 norovirus sequences from 1974 to 2014 to identify converging sites. Variable sites were proportionally more likely to be within two amino acids (P < 0.05) of positively selected sites. Further analysis using a hypergeometric distribution indicated that polymorphic site distribution was random and its proximity to positively selected sites was dependent on the size of the norovirus genome and the number of positively selected sites.In conclusion, random mutations may have a positive impact on driving norovirus evolution, and immunocompromised individuals could serve as potential reservoirs for novel GII.4 strains.
PI-Transporter Requirements as Enabler for the Implementation of the Road-Based Physical Internet
Creators: name: Kaup, Steffen
name: Ludwig, André
name: Franczyk, Bogdan
Publisher: name: Georgia Institute of Technology
Year: 2023
Subjects: PI-Transporter requirements
Road-based physical internet (RBPI)
Cargo space fitting

description: Presented at the 9th International Physical Internet Conference (IPIC 2023) 13-15 June 2023, Athens, Greece
description: The contribution of this research Poster to the Physical Internet (PI) is to present PI-Transporter functionality that enables vehicles to take part in the roadbased Physical Internet (RBPI). The requirements are derived from detailed protocol analyses and applied to road-based PI-Transporters [1, 3]. This ranges from remote access to the cargo space of PI-Transporters, cargo securing through an adaptive 3D surface cargo space interior, an automated vacant cargo space detection, up to a standardized interface for V2X communication in order to negotiate freight forwarding within the road-based vehicle network.
Additional file 1 of Oxindole–benzothiazole hybrids as CDK2 inhibitors and anticancer agents: design, synthesis and biological evaluation
Creators: name: Abdel-Mohsen, Heba T.
Publisher: name: figshare
Year: 2024
Subjects: subject: Biophysics
subject: Biochemistry
subject: Physical Sciences not elsewhere classified
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Molecular Biology
subject: Pharmacology
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Cancer
subject: Hematology

description: Supplementary Material 1. (1) NMR Spectra of oxindole–benzothiazole hybrids 9a–r. (2) IR charts of the synthesized oxindole–benzothiazoles. (3) Screening of cytotoxic activity against a panel of sixty human tumor cell lines. (4) One dose mean graphs of the oxindole–benzothiazoles. (5) Dose response curve of 9o on NCI cancer cell lines. (6) Analysis of cell cycle distribution. (7) Apoptosis assay. (8) Biochemical kinase assay procedure. (9) Docking of the co-crystalized ligand in the binding site of CDK2. (10) Bioavailability radar charts for 9a–r from SwissADME free webtool. (11) References.