Additional file 1 of Aging and high-fat diet feeding lead to peripheral insulin resistance and sex-dependent changes in brain of mouse model of tau pathology THY-Tau22
Creators: name: Kacířová, Miroslava
name: Železná, Blanka
name: Blažková, Michaela
name: Holubová, Martina
name: Popelová, Andrea
name: Kuneš, Jaroslav
name: Šedivá, Blanka
name: Maletínská, Lenka
Publisher: name: figshare
Year: 2021
Subjects: subject: Biochemistry
subject: Cell Biology
subject: Molecular Biology
subject: Neuroscience
subject: Physiology
subject: FOS: Biological sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: 111714 Mental Health
subject: FOS: Health sciences
subject: Computational Biology

description: Additional file 1: Fig. S1. Behavioral test. Open field (n = 7-14). Data are presented as mean ± SEM and were statistically analyzed by Mann-Whitney t-test (*) within each age and sex group (*p &lt; 0.05; **p &lt; 0.01). The age comparison was performed by mixed-effects analysis and Bonferroni’s post hoc test (#). A value significance between 11 and 3, and 11 and 7 months in particular group at wall distance was #p &lt; 0.001 in all cases (not shown for clarity). Fig. S2 Western blots of hippocampal microgliosis marker CD11b. (A) Western blots for specific proteins. (B) Quantification of (A) western blots. Data are presented as mean ± SEM and were statistically analyzed by Mann-Whitney t-test within each age and sex group (*p &lt; 0.05; n = 6). Mouse wt group on St diet was set as 100 %. The intensity of all proteins was related to particular β-actin intensity. CD11b: cluster of differentiation molecule 11b known as integrin alpha M; Iba1: ionized calcium-binding adaptor molecule 1. Fig. S3 Western blots of hippocampal insulin signaling pathway markers of 11-month-old THY-Tau22 and wt mice. (A) Western blots for specific proteins. (B) Quantification of (A) western blots. Data are presented as mean ± SEM and were statistically analyzed by Mann-Whitney t-test within each age and sex group (*p &lt; 0.05; **p &lt; 0.01; n = 6). Mouse wt group on St diet was set as 100 %. The intensity of all proteins was related to particular β-actin intensity. Akt: protein kinase B; GSK-3β: glycogen synthase kinase 3 beta; PDK-1: phosphoinositide-dependent kinase-1. Fig. S4 Western blots of hippocampal ERK1/2 of 11-month-old THY-Tau22 and wt mice. (A) Western blots for specific proteins. (B) Quantification of (A) western blots. Data are presented as mean ± SEM and were statistically analyzed by Mann-Whitney t-test within each age and sex group (**p &lt; 0.01; n = 6). Mouse THY-Tau22 group on St diet was set as 100 %. The intensity of all proteins was related to particular β-actin intensity. ERK1/2: extracellular signal regulated kinase. Fig. S5 Western blots of hippocampal Tau5 of 11-month-old THY-Tau22 mice. (A) Western blots for specific proteins. (B) Quantification of (A) western blots. Data are presented as mean ± SEM and were statistically analyzed using Mann-Whitney t-test. No statistically significant difference was observed. (p ≥ 0.05; n = 6). Mouse THY-Tau22 group on St diet was set as 100 %. The intensity of Tau5 was related to particular β-actin intensity.