title: ÐÑÐ¾Ð±ÐµÐ½Ð½Ð¾ÑÑÐ¸ Ð¼ÐµÑÐ¾Ð´Ð¸Ðº Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð±Ð¸Ð¾Ð¼Ð°ÑÐºÐµÑÐ¾Ð² Ð² ÑÐ°Ð½Ð½ÐµÐ¹ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐµ Ð³Ð»Ð¾Ð¼ÐµÑÑÐ»Ð¾Ð½ÐµÑÑÐ¸ÑÐ¾Ð²
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Peculiarities of renal injury markers detection methods in blood and urine in patients with early stages of primary glomerulopathies
Creators: name: ÐÐ°ÑÑÐ³Ð¸Ð½Ð°, ÐÐ°ÑÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð¿ÐµÑÐ²Ð¸ÑÐ½ÑÐµ Ð³Ð»Ð¾Ð¼ÐµÑÑÐ»Ð¾Ð¿Ð°ÑÐ¸Ð¸
Ð¼Ð°ÑÐºÐµÑÑ Ð¿Ð¾Ð²ÑÐµÐ¶Ð´ÐµÐ½Ð¸Ñ Ð¿Ð¾ÑÐµÐº
Ð¼ÐµÑÐ¾Ð´Ñ ÐºÐ»Ð¸Ð½Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ½Ð¾Ð¹ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐ¸
ÑÑÐ²Ð¾ÑÐ¾ÑÐ¾ÑÐ½ÑÐ¹ ÑÐ¸ÑÑÐ°ÑÐ¸Ð½ C
ÐºÑÐµÐ°ÑÐ¸Ð½Ð¸Ð½
Ð²Ð¸ÑÐ°Ð¼Ð¸Ð½ D
Ñ ÑÐ¾Ð½Ð¸ÑÐµÑÐºÐ°Ñ Ð±Ð¾Ð»ÐµÐ·Ð½Ñ Ð¿Ð¾ÑÐµÐº
Ð»Ð¸Ð¿Ð¾ÐºÐ°Ð»Ð¸Ð½-2 Ð² Ð¼Ð¾ÑÐµ
Ð°Ð»ÑÐ±ÑÐ¼Ð¸Ð½ Ð² Ð¼Ð¾ÑÐµ
Ð¾Ð±ÑÐ¸Ð¹ Ð±ÐµÐ»Ð¾Ðº Ð¼Ð¾ÑÐ¸
primary glomerulopathies
markers of kidney demage. clinical laboratory diagnostic methods
serum cystatin C. creatinine. vitamin D
chronic kidney disease
urinary lipocalin-2
urinary albumin
total urinary protein

description: Ð¦ÐµÐ»Ñ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ â Ð¾Ð¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ñ Ð¼Ð°ÑÐºÐµÑÐ¾Ð² Ð¿Ð¾Ð²ÑÐµÐ¶Ð´ÐµÐ½Ð¸Ñ Ð¿Ð¾ÑÐµÐº Ð² ÐºÑÐ¾Ð²Ð¸ Ð¸ Ð¼Ð¾ÑÐµ Ñ Ð¿Ð°ÑÐ¸ÐµÐ½ÑÐ¾Ð² Ñ ÑÐ°Ð½Ð½Ð¸Ð¼Ð¸ ÑÑÐ°Ð´Ð¸ÑÐ¼Ð¸ Ð¿ÐµÑÐ²Ð¸ÑÐ½ÑÑ Ð³Ð»Ð¾Ð¼ÐµÑÑÐ»Ð¾Ð¿Ð°ÑÐ¸Ð¹ Ð¼ÐµÑÐ¾Ð´Ð°Ð¼Ð¸ ÐºÐ»Ð¸Ð½Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ½Ð¾Ð¹ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐ¸ Ð´Ð»Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ Ð² Ð¾Ð±Ð»Ð°ÑÑÐ¸ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð¼ÐµÐ´Ð¸ÑÐ¸Ð½Ñ Ð² ÑÐ°Ð¼ÐºÐ°Ñ Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð¸ Ð¿ÑÐ¾ÑÐ¸Ð»Ð°ÐºÑÐ¸ÐºÐ¸ ÑÐ°Ð½Ð½Ð¸Ñ Ð³Ð»Ð¾Ð¼ÐµÑÑÐ»Ð¾Ð½ÐµÑÑÐ¸ÑÐ¾Ð² Ð¿ÑÐ¸ ÑÑÐ¾Ð½Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ Ð¿Ð¾ÑÐµÐº. ÐÐ»Ñ Ð´Ð¾ÑÑÐ¸Ð¶ÐµÐ½Ð¸Ñ ÑÐµÐ»Ð¸ Ð±ÑÐ»Ð¸ Ð¿Ð¾ÑÑÐ°Ð²Ð»ÐµÐ½Ñ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð·Ð°Ð´Ð°ÑÐ¸: 1) ÐÑÐ²Ð¾Ð¸ÑÑ Ð¸ Ð¾ÑÑÑÐµÑÑÐ²Ð¸ÑÑ Ð¿ÑÐ¾Ð±Ð¾Ð¿Ð¾Ð´Ð³Ð¾ÑÐ¾Ð²ÐºÑ Ð¾Ð±ÑÐ°Ð·ÑÐ¾Ð² Ð¼Ð¾ÑÐ¸ Ð¸ ÑÑÐ²Ð¾ÑÐ¾ÑÐºÐ¸ ÐºÑÐ¾Ð²Ð¸ Ð¿Ð°ÑÐ¸ÐµÐ½ÑÐ¾Ð² Ñ Ð³Ð»Ð¾Ð¼ÐµÑÑÐ»Ð¾Ð¿Ð°ÑÐ¸ÑÐ¼Ð¸ Ð¸ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½Ð¾Ð¹ Ð³ÑÑÐ¿Ð¿Ñ. 2) ÐÐ²Ð»Ð°Ð´ÐµÑÑ Ð¼ÐµÑÐ¾Ð´Ð°Ð¼Ð¸ ÑÐ¾ÑÐ¾Ð¼ÐµÑÑÐ¸Ð¸, ÑÑÑÐ±Ð¸Ð´Ð¸Ð¼ÐµÑÑÐ¸Ð¸, Ð¸Ð¼Ð¼ÑÐ½Ð¾ÑÐµÑÐ¼ÐµÐ½ÑÐ½Ð¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð°. 3) ÐÐ·Ð¼ÐµÑÐ¸ÑÑ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ Ð¼Ð°ÑÐºÐµÑÐ¾Ð² Ð² Ð¾Ð±ÑÐ°Ð·ÑÐ°Ñ ÐºÑÐ¾Ð²Ð¸ Ð¸ Ð¼Ð¾ÑÐ¸ Ð¿Ð°ÑÐ¸ÐµÐ½ÑÐ¾Ð² Ð¼ÐµÑÐ¾Ð´Ð°Ð¼Ð¸ ÑÐ¾ÑÐ¾Ð¼ÐµÑÑÐ¸Ð¸, Ð¸Ð¼Ð¼ÑÐ½Ð¾ÑÑÑÐ±Ð¸Ð´Ð¸Ð¼ÐµÑÑÐ¸Ð¸ Ð¸ Ð¸Ð¼Ð¼ÑÐ½Ð¾ÑÐµÑÐ¼ÐµÐ½ÑÐ½Ð¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð°. 4) ÐÑÐ¾Ð²ÐµÑÑÐ¸ ÑÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð°Ð½Ð°Ð»Ð¸Ð· Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð´Ð°Ð½Ð½ÑÑ. Ð£ÑÐ¾Ð²ÐµÐ½Ñ Ð»Ð¸Ð¿Ð¾ÐºÐ°Ð»Ð¸Ð½Ð°-2, Ð²Ð¸ÑÐ°Ð¼Ð¸Ð½Ð° D Ð¸Ð·Ð¼ÐµÑÑÐ»Ð¸ Ð¸Ð¼Ð¼ÑÐ½Ð¾ÑÐµÐ¼Ð¸Ð»ÑÐ¼Ð¸Ð½ÐµÑÑÐµÐ½ÑÐ½ÑÐ¼ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð½Ð° Ð°Ð½Ð°Ð»Ð¸Ð·Ð°ÑÐ¾ÑÐµ ARCHITECT i2000SR (Abbott, Ð¡Ð¨Ð). Ð£ÑÐ¾Ð²ÐµÐ½Ñ Ð¾Ð±ÑÐµÐ³Ð¾ Ð±ÐµÐ»ÐºÐ°, Ð°Ð»ÑÐ±ÑÐ¼Ð¸Ð½Ð°, ÐºÑÐµÐ°ÑÐ¸Ð½Ð¸Ð½Ð°, ÑÐ¸ÑÑÐ°ÑÐ¸Ð½Ð° C Ð½Ð° Ð±Ð¸Ð¾ÑÐ¸Ð¼Ð¸ÑÐµÑÐºÐ¾Ð¼ Ð°Ð²ÑÐ¾Ð¼Ð°ÑÐ¸ÑÐµÑÐºÐ¾Ð¼ Ð°Ð½Ð°Ð»Ð¸Ð·Ð°ÑÐ¾ÑÐµ CÐ 90 (Furuno, Ð¯Ð¿Ð¾Ð½Ð¸Ñ).
description: The aim of the graduate qualification work is to define the concentration of renal injury markers in blood and urine in patients with early stages of primary glomerulopathies by methods of clinical laboratory diagnostics for research in the field of experimental medicine within the framework of research and prevention of early glomerulonephritis in chronic kidney disease. To achieve the aim, the following goals were set: 1) Learn and carry out sample preparation of urine and serum samples of patients with glomerulopathies and control group. 2) Learn the methods of photometry, turbidimetry, and enzyme immunoassay. 3) Measure the concentrations of markers in serum and urine samples of patients with early stages of glomerulopathies by methods of photometry, immunoturbidimetry and enzyme immunoassay. 4) Perform statistical analysis of the data. Lipocalin-2, vitamin D were measured by immunochemiluminescent method on ARCHITECT i2000SR analyzer (Abbott, USA). The total protein, albumin, creatinine, and cystatin C levels were measured on a biochemical automatic analyzer CA 90 (Furuno, Japan).