cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Targeted Genome Sequencing (TG-Seq) Approaches to Detect Plant Viruses
Creators: name: S Maina
name: L Zheng
name: Rodoni, Brendan
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Globally, high-throughput sequencing (HTS) has been used for virus detection in germplasm certification programs. However, sequencing costs have impeded its implementation as a routine diagnostic certification tool. In this study, the targeted genome sequencing (TG-Seq) approach was developed to simultaneously detect multiple (four) viral species of; Pea early browning virus (PEBV), Cucumber mosaic virus (CMV), Bean yellow mosaic virus (BYMV) and Pea seedborne mosaic virus (PSbMV). TG-Seq detected all the expected viral amplicons within multiplex PCR (mPCR) reactions. In contrast, the expected PCR amplicons were not detected by gel electrophoresis (GE). For example, for CMV, GE only detected RNA1 and RNA2 while TG-Seq detected all the three RNA components of CMV. In an mPCR to amplify all four viruses, TG-Seq readily detected each virus with more than 732,277 sequence reads mapping to each amplicon. In addition, TG-Seq also detected all four amplicons within a 10-8 serial dilution that were not detectable by GE. Our current findings reveal that the TG-Seq approach offers significant potential and is a highly sensitive targeted approach for detecting multiple plant viruses within a given biological sample. This is the first study describing direct HTS of plant virus mPCR products. These findings have major implications for grain germplasm healthy certification programs and biosecurity management in relation to pathogen entry into Australia and elsewhere.
Access route selection for percutaneous coronary intervention among Vietnamese patients: Implications for in-hospital costs and outcomes
Creators: name: HTT Vu
name: R Norman
name: NM Pham
name: HM Pham
name: HTT Nguyen
name: QN Nguyen
name: LD Do
name: RR Huxley
name: Lee, Crystal
name: TM Hoang
name: CM Reid
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Background: Little is known about rates of access site (transradial (TRI) or transfemoral (TFI)) preference for percutaneous coronary intervention (PCI) and in-hospital costs of patients undergoing these procedures in lower-and middle-countries. Here, we report on access site use, in-hospital costs and outcomes of patients undergoing PCI in Vietnam. Methods: Information from 868 patients were included in the cohort of 1022 patients recruited into the first PCI registry in Vietnam. The total hospital costs and in-hospital outcomes of patients undergoing TRI and TFI were compared. Hospital costs were obtained from the hospital admission system, and major adverse cardiac events, major bleeding events and length of stay were identified through review of medical records. Findings: TRI was the dominant access site for interventionists (694/868 patients). The TFI group reported more lesions of the left main artery, more previous coronary artery bypass grafts and previous PCI in comparison with the TRI group (all p < 0.05). The TRI group was associated with a lower overall cost of admission (the adjusted difference was -1526.3 USD, 95% confident interval CI (-1996.2; -1056.3), shorter length of hospital stay (-2 days, CI (-2.8; -1.2)) and lower rates of major bleeding post-procedure. Procedural factors such as radial access site, left main disease, PCI ≥2 stents, and PCI ≥ 2 lesions having the most impact on the in-hospital cost of patients undergoing PCI. Interpretations: Among patients undergoing PCI, TRI was associated with lower costs and favourable clinical outcomes relative to TFI Funding: This research received partial financial support from Curtin University, Australia.
Synthesis and characterization of silver-strontium (Ag-Sr)-doped mesoporous bioactive glass nanoparticles
Creators: name: Bano, S
name: Akhtar, M
name: Yasir, M
name: Maqbool, MS
name: Niaz, A
name: Wadood, A
name: Rehman, MAU
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Biomedical implants are the need of this era due to the increase in number of accidents and follow-up surgeries. Different types of bone diseases such as osteoarthritis, osteomalacia, bone cancer, etc., are increasing globally. Mesoporous bioactive glass nanoparticles (MBGNs) are used in biomedical devices due to their osteointegration and bioactive properties. In this study, silver (Ag)- and strontium (Sr)-doped mesoporous bioactive glass nanoparticles (Ag-Sr MBGNs) were prepared by a modified Stöber process. In this method, Ag and Sr were co-substituted in pure MBGNs to harvest the antibacterial properties of Ag ions, as well as pro-osteogenic potential of Sr ions. The effect of the two-ion concentration on morphology, surface charge, composition, antibacterial ability, and in-vitro bioactivity was studied. Scanning electron microscopy (SEM), X-Ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR) confirmed the doping of Sr and Ag in MBGNs. SEM and EDX analysis confirmed the spherical morphology and typical composition of MBGNs, respectively. The Ag-Sr MBGNs showed a strong antibacterial effect against Staphylococcus carnosus and Escherichia coli bacteria determined via turbidity and disc diffusion method. Moreover, the synthesized Ag-Sr MBGNs develop apatite-like crystals upon immersion in simulated body fluid (SBF), which suggested that the addition of Sr improved in vitro bioactivity. The Ag-Sr MBGNs synthesized in this study can be used for the preparation of scaffolds or as a filler material in the composite coatings for bone tissue engineering. + 2+ 2
Antivirulence DsbA inhibitors attenuate Salmonella enterica serovar Typhimurium fitness without detectable resistance
Creators: name: Rabeb Dhouib
name: Vagenas, Dimitrios
name: Yaoqin Hong
name: Verderosa, Anthony D
name: Martin, Jennifer L
name: Begona Heras
name: Totsika, Makrina
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Inhibition of the DiSulfide Bond (DSB) oxidative protein folding machinery, a major facilitator of virulence in Gram-negative bacteria, represents a promising antivirulence strategy. We previously developed small molecule inhibitors of DsbA from Escherichia coli K-12 (EcDsbA) and showed that they attenuate virulence of Gram-negative pathogens by directly inhibiting multiple diverse DsbA homologues. Here we tested the evolutionary robustness of DsbA inhibitors as antivirulence antimicrobials against Salmonella enterica serovar Typhimurium under pathophysiological conditions in vitro. We show that phenylthiophene DsbA inhibitors slow S. Typhimurium growth in minimal media, phenocopying S. Typhimurium isogenic dsbA null mutants. Through passaging experiments, we found that DsbA inhibitor resistance was not induced under conditions that rapidly induced resistance to ciprofloxacin, an antibiotic commonly used to treat Salmonella infections. Furthermore, no mutations were identified in the dsbA gene of inhibitor-treated S. Typhimurium, and S. Typhimurium virulence remained susceptible to DsbA inhibitors. Our work demonstrates that under in vitro pathophysiological conditions, DsbA inhibitors can have both antivirulence and antibiotic action. Importantly, our finding that DsbA inhibitors appear to be evolutionarily robust offers promise for their further development as next-generation antimicrobials against Gram-negative pathogens.
Do hip and groin muscle strength and symptoms change throughout a football season in professional male football players? A prospective cohort study with repeated measures
Creators: name: van Klij, P
name: Langhout, R
name: van Beijsterveldt, AMC
name: Stubbe, JH
name: Weir, A
name: Agricola, R
name: Fokker, Y
name: Mosler, Andrea
name: Waarsing, JH
name: Verhaar, J
name: Tak, I
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Objectives: Groin injuries are common in professional male football and result in significant complaints, time-loss and cost. We aimed to study: 1. Normal values of hip muscle strength and self-reported hip and groin function (Hip And Groin Outcome Score (HAGOS)). 2. Changes in these values throughout the season. 3. If previous (groin) injuries, leg dominance or league were associated with these outcome measures. Design: Prospective cohort study. Methods: 313 professional male football players (11 clubs) participated. Player characteristics and previous injuries were registered. Hip muscle strength (hand-held dynamometer) and HAGOS measurements were done at the start, middle and end of the season. Results: Data from 217 players were analysed. Adduction strength (mean ± standard deviation, Nm/Kg) was 3.40 ± 0.72 (start), 3.30 ± 0.65 (mid) and 3.39 ± 0.74 (end) (p = 0.186). Abduction strength was 3.45 ± 0.67, 3.14 ± 0.57 and 3.28 ± 0.61 (p < 0.001). Adduction/abduction ratio was 1.00 ± 0.21, 1.07 ± 0.22 and 1.05 ± 0.23 (p < 0.001). Statistically, the HAGOS-subscale ‘Pain’ (median [interquartile range]) deteriorated slightly during the season (p = 0.005), especially from mid-season (97.5 [90.6−100.0]) to end-of-season (95.0 [87.5−100.0]) (p = 0.003). Other subscale scores remained unchanged between time points; 85.7 (symptoms), 100.0 (daily living), 96.9 (sports and recreation) 100.0, (physical activities) and 90.0 (quality of life). Previous injuries were associated with lower HAGOS-scores. Dominant legs had higher abduction strength (p < 0.001) and lower adduction/abduction ratio (p < 0.001). No differences between leagues were found for hip muscle strength and HAGOS-scores. Conclusions: In Dutch male professional football players, hip muscle strength and HAGOS-scores remained relatively stable throughout the season. Pain increased slightly, which while statistically significant, was not clinically relevant.
Revisiting Milgram and Kishino's Reality-Virtuality Continuum
Creators: name: Skarbez, Richard
name: Missie Smith
name: Whitton, Mary C
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Since its introduction in 1994, Milgram and Kishino's reality-virtuality (RV) continuum has been used to frame virtual and augmented reality research and development. While originally, the RV continuum and the three dimensions of the supporting taxonomy (extent of world knowledge, reproduction fidelity, and extent of presence metaphor) were intended to characterize the capabilities of visual display technology, researchers have embraced the RV continuum while largely ignoring the taxonomy. Considering the leaps in technology made over the last 25 years, revisiting the RV continuum and taxonomy is timely. In reexamining Milgram and Kishino's ideas, we realized, first, that the RV continuum is actually discontinuous; perfect virtual reality cannot be reached. Secondly, mixed reality is broader than previously believed, and, in fact, encompasses conventional virtual reality experiences. Finally, our revised taxonomy adds coherence, accounting for the role of users, which is critical to assessing modern mixed reality experiences. The 3D space created by our taxonomy incorporates familiar constructs such as presence and immersion, and also proposes new constructs that may be important as mixed reality technology matures.
Additional file 1 of Knowledge and skills of pre-eclampsia management among healthcare providers working in antenatal clinics in Zanzibar
Creators: name: Seif, Saada Ali
name: Rashid, Salma Ali
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Cell Biology
subject: Biotechnology
subject: Sociology
subject: FOS: Sociology

description: Additional file 1.
Additional file 1 of Retrospective review of the epidemiology, microbiology, management and outcomes of intra-cranial abscesses at a neurosurgical tertiary referral centre, 2018–2020
Creators: name: Evans, Terry John
name: Jawad, Sarah
name: Kalyal, Nida
name: Nadarajah, Angelina
name: Amarouche, Meriem
name: Stapleton, Simon
name: Ward, Christopher
name: Breathnach, Aodhan
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Neuroscience
subject: Pharmacology
subject: Cancer
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Computational Biology

description: Additional file 1. Antibiotic guideline for the treatment of intracranial abscesses used in our hospital.
Supplemental Material, Todaka_suppl_dia - Impact of Risk-Benefit Perception and Trust on Medical Technology Acceptance in Relation to Drug and Device Lag: A Tripartite Cross-Sectional Survey
Creators: name: Todaka, Koji
name: Kishimoto, Junji
name: Ikeda, Masayuki
name: Ikeda, Koji
name: Yamamoto, Haruko
Publisher: name: SAGE Journals
Year: 2017
Subjects: subject: 111599 Pharmacology and Pharmaceutical Sciences not elsewhere classified
subject: FOS: Clinical medicine

description: Supplemental Material, Todaka_suppl_dia for Impact of Risk-Benefit Perception and Trust on Medical Technology Acceptance in Relation to Drug and Device Lag: A Tripartite Cross-Sectional Survey by Koji Todaka, Junji Kishimoto, Masayuki Ikeda, Koji Ikeda, and Haruko Yamamoto in Therapeutic Innovation & Regulatory Science
CRISPR/Cas9-mediated knockout of GmFATB1 significantly reduced the amount of saturated fatty acids in soybean seeds
Creators: name: J Ma
name: S Sun
name: Whelan, James
name: H Shou
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Soybean (Glycine max) oil is one of the most widely used vegetable oils across the world. Breeding of soybean to reduce the saturated fatty acid (FA) content, which is linked to cardiovascular disease, would be of great significance for nutritional improvement. Acyl-acyl carrier protein thioesterases (FATs) can release free FAs and acyl-ACP, which ultimately affects the FA profile. In this study, we identified a pair of soybean FATB coding genes, GmFATB1a and GmFATB1b. Mutants that knock out either or both of the GmFATB1 genes were obtained via CRISPR/Cas9. Single mutants, fatb1a and fatb1b, showed a decrease in leaf palmitic and stearic acid contents, ranging from 11% to 21%. The double mutant, fatb1a:1b, had a 42% and 35% decrease in palmitic and stearic acid content, displayed growth defects, and were male sterility. Analysis of the seed oil profile revealed that fatb1a and fatb1b had significant lower palmitic and stearic acid contents, 39–53% and 17–37%, respectively, while that of the unsaturated FAs were the same. The relative content of the beneficial FA, linoleic acid, was increased by 1.3–3.6%. The oil profile changes in these mutants were confirmed for four generations. Overall, our data illustrate that GmFATB1 knockout mutants have great potential in improving the soybean oil quality for human health.
Additional file 2 of Ultra-lung-protective ventilation and biotrauma in severe ARDS patients on veno-venous extracorporeal membrane oxygenation: a randomized controlled study
Creators: name: Guervilly, Christophe
name: Fournier, Théotime
name: Chommeloux, Juliette
name: Arnaud, Laurent
name: Pinglis, Camille
name: Baumstarck, Karine
name: Boucekine, Mohamed
name: Valera, Sabine
name: Sanz, Celine
name: Adda, Mélanie
name: Bobot, Mickaël
name: Daviet, Florence
name: Gragueb-Chatti, Ines
name: Forel, Jean-Marie
name: Roch, Antoine
name: Hraiech, Sami
name: Dignat-George, Françoise
name: Schmidt, Matthieu
name: Lacroix, Romaric
name: Papazian, Laurent
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Cell Biology
subject: Physiology
subject: FOS: Biological sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: Cancer
subject: Mental Health

description: Additional file 2. Online data supplement.
Is natural fraxin an overlooked radical scavenger?
Creators: name: Nam, PC
name: Thong, NM
name: Hoa, NT
name: Quang, DT
name: Hoang, LP
name: Mechler, Adam
name: Vo, Quan Van
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Fraxin (FX) (7-hydroxy-6-methoxycoumarin 8-glucoside) is a typical natural product of the coumarin family. This compound was shown to protect endothelial cells from oxidative stress; however, the nature of its antioxidant properties is still ambiguous. In this study, we report on a systematic evaluation of the radical scavenging activity of FX using a two-tier protocol based on thermodynamic and kinetic calculations. The results show that FX has moderate activity in the aqueous physiological environment against a range of radicals including HO, CCl3O, CCl3OO, NO2, and HOO. The latter was examined in detail due to the prevalence of HOO as a source of oxidative stress in biological systems. HOO scavenging activity was promising in the gas phase but low in physiological environments with koverall = 1.57 × 106, 3.13 × 102 and 2.68 × 103 M-1 s-1 in the gas phase, pentyl ethanoate and water solvents, respectively. The formal hydrogen transfer mechanism at the O7-H bond dominates the hydroperoxyl radical scavenging of FX in the nonpolar media, whereas, in the polar environment, the activity is exerted by the single electron transfer mechanism of the anion state. This activity falls behind typical antioxidants such as Trolox, ascorbic acid, and trans-resveratrol under the studied conditions. Thus FX may have multiple health benefits, but it is not an outstanding natural antioxidant. This journal is
Two transcriptionally distinct pathways drive female development in a reptile with both genetic and temperature dependent sex determination
Creators: name: SL Whiteley
name: CE Holleley
name: S Wagner
name: J Blackburn
name: IW Deveson
name: Graves, Jennifer
name: A Georges
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: How temperature determines sex remains unknown. A recent hypothesis proposes that conserved cellular mechanisms (calcium and redox; 'CaRe' status) sense temperature and identify genes and regulatory pathways likely to be involved in driving sexual development. We take advantage of the unique sex determining system of the model organism, Pogona vitticeps, to assess predictions of this hypothesis. P. vitticeps has ZZ male: ZW female sex chromosomes whose influence can be overridden in genetic males by high temperatures, causing male-to-female sex reversal. We compare a developmental transcriptome series of ZWf females and temperature sex reversed ZZf females. We demonstrate that early developmental cascades differ dramatically between genetically driven and thermally driven females, later converging to produce a common outcome (ovaries). We show that genes proposed as regulators of thermosensitive sex determination play a role in temperature sex reversal. Our study greatly advances the search for the mechanisms by which temperature determines sex.
Prediction of Johne’s disease state based on quantification of T cell markers and their interaction with macrophages in the bovine intestine
Creators: name: Jenvey, Caitlin
name: AL Shircliff
name: E Obando Marrero
name: JR Stabel
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Cell-mediated immune responses to Mycobacterium avium subsp. paratuberculosis (MAP) are regulated by various types of T lymphocytes. The aim of this study was to quantitate T cell subsets in the mid-ileum of cows naturally infected with MAP to identify differences during different stages of infection, and to determine whether these subsets could be used as predictors of disease state. Immunofluorescent labeling of T cell subsets and macrophages was performed on frozen mid-ileal tissue sections archived from naturally infected dairy cows in either subclinical or clinical disease status, and noninfected control cows. Comprehensive IF staining for CD4, CD8α, TcR1-N24 (gamma delta), FoxP3, CXCR3 and CCR9 served to define T cell subsets and was correlated with macrophages present. Clinically affected cows demonstrated significantly higher numbers of CXCR3 (Th1-type) and CCR9 (total small intestinal lymphocytes) cells at the site of infection compared to the subclinical cows and noninfected controls. Further, predictive modeling indicated a significant interaction between CXCR3 and AM3K (macrophages) cells, suggesting that progression to clinical disease state aligns with increased numbers of these cell types at the site of infection. The ability to predict disease state with this model was improved from previous modeling using immunofluorescent macrophage data. Predictive modelling indicated an interaction between CXCR3 and AM3K cells, which could more sensitively detect subclinical cows compared to clinical cows. It may be possible to use this knowledge to improve and develop an assay to detect subclinically infected animals with more confidence during the early stages of the disease. + + + + + +
How Many Patients Achieve an Acceptable Symptom State After Hip Arthroscopy for Femoroacetabular Impingement Syndrome? A Cross-sectional Study Including PASS Cutoff Values for the HAGOS and iHOT-33
Creators: name: L Ishøi
name: K Thorborg
name: MG Ørum
name: Kemp, Joanne
name: MP Reiman
name: P Hölmich
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Background: Hip arthroscopy is a viable treatment for femoroacetabular impingement syndrome (FAIS). Clinically relevant improvements in hip function and pain after surgery are often reported, but it is less clear how many patients achieve an acceptable symptom state (Patient Acceptable Symptom State [PASS]). Purpose: To investigate the proportion of patients who achieved a PASS 12 to 24 months after hip arthroscopy and to determine the cutoff scores of the 2 recommended and valid patient-reported outcome measures (the subscales of the Copenhagen Hip and Groin Outcome Score [HAGOS] and the International Hip Outcome Tool—33 [iHOT-33]) for which patients are most likely to achieve PASS. Study Design: Cohort study; Level of evidence, 3. Methods: Eligible study patients were identified in the Danish Hip Arthroscopy Registry. An electronic questionnaire was used to collect data on PASS, HAGOS, and iHOT-33 12 to 24 months after surgery. PASS was measured using an anchor question. Receiver operating characteristic curve analyses were applied to identify the PASS cutoff values of HAGOS and iHOT-33 scores. Results: A total of 137 individuals (mean age at surgery, 35.4 ± 9.4 years) were included in the study at a mean follow-up of 18.5 ± 3.2 months after surgery. At follow-up, 64 individuals (46.7%; 95% CI, 38.6-55.1) reported PASS. Higher HAGOS and iHOT-33 values were observed for participants who reported PASS compared with those who did not report PASS (Cohen d ≥ 1.06; P
Sleep and performance during a preseason in elite rugby union athletes
Creators: name: Teece, Angus R
name: Argus, Christos K
name: Gill, Nicholas
name: Beaven, Martyn
name: Dunican, Ian C
name: Driller, Matthew
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Background: Preseason training optimises adaptations in the physical qualities required in rugby union athletes. Sleep can be compromised during periods of intensified training. Therefore, we investigated the relationship between sleep quantity and changes in physical performance over a preseason phase in professional rugby union athletes. Methods: Twenty-nine professional rugby union athletes (Mean ± SD, age: 23 ± 3 years) had their sleep duration monitored for 3 weeks using wrist actigraphy. Strength and speed were assessed at baseline and at week 3. Aerobic capacity and body composition were assessed at baseline, at week 3 and at week 5. Participants were stratified into 2 groups for analysis: <7 h 30 min sleep per night (LOW, n = 15) and >7 h 30 min sleep per night (HIGH, n = 14). Results: A significant group x time interaction was determined for aerobic capacity (p = 0.02, d = 1.25) at week 3 and for skinfolds at week 3 (p < 0.01, d = 0.58) and at week 5 (p = 0.02, d = 0.92), in favour of the HIGH sleep group. No differences were evident between groups for strength or speed measures (p ≥ 0.05). Conclusion: This study highlights that longer sleep duration during the preseason may assist in enhancing physical qualities including aerobic capacity and body composition in elite rugby union athletes.
Prevalence of substance use among psychotic patients and determining its strongest predictor
Creators: name: SB Mousavi
name: Higgs, Peter
name: N Piri
name: E Sadri
name: M Pourghasem
name: SJ Fakhari
name: M Noroozi
name: M Miladinia
name: E Ahounbar
name: A Sharhani
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Objective: Although comorbidity of psychotic disorders and substance use can lead to increase in mortality, less is known about the outbreak and predictors. Psychotic patients tend to be overlooked during assessment; hence, the possibility of an undertreated or missed condition such as increasing substance use. This investigation aimed to measure the prevalence of substance use in psychotic patients and to survey the powerful predictors. Method: In a 1-year cross-sectional study, 311 psychotic patients were assessed using the Structured Interview Based on DSM-5 for diagnostic confirmation as well as questions surveying prevalence and possible predictors of substance use. Results: Prevalence of substance use among psychotic patients was 37.9%. Several variables were identified as factors associated with drug abuse among the psychotic patients. These included male gender, younger age, being currently homeless, a history of imprisonment, and having family history of drug use. The strongest predictors of substance use, however, were family history of drug use, male gender, and being currently homelessness. Conclusion: Policymakers should note the importance of substance use among psychotic patients. Developing active screening strategies and comprehensive preventive plans, especially in the high-risk population, is suggested.
Εργαστηριακές Ασκήσεις για το Φυσικό Επίπεδο στα Ασύρματα Δίκτυα
Creators: name: Τσιόφας, Νικόλαος Δ.
Publisher: name: Aristotle University of Thessaloniki
Year: 2025
Subjects: Ασύρματη επικοινωνία
Ασύρματο δίκτυο
Πρωτόκολλα ασφαλείας
Άρνηση παροχής υπηρεσιών (DoS)
Εξουσιοδότηση
Wireless communication
Wireless network
Security Protocols
WPA
Denial of Service (DoS)
Authorization

description: Η εργασία εστιάζει πάνω στο εργαλείο του RF Propagation Calculator και προσφέρει έναν πλήρη οδηγό βήμα προς βήμα για την χρήση και τις λειτουργίες του λογισμικού. Το αναλυτικό εγχειρίδιο, συνοδεύεται από ένα σύνολο εργαστηριακών ασκήσεων πάνω στο εργαλείο του RF Propagation Calculator, οι οποίες θεμελιώνονται πάνω σε αρχιτεκτονικές και τεχνολογίες του Φυσικού επίπεδο (Psysical Layer) των Ασύρματων Δικτύων. Επίσης, αναλύονται οι τεχνικές και οι τεχνολογίες που χρησιμοποιούνται για την επίτευξη αποδοτικής και αξιόπιστης επικοινωνίας μέσω των ραδιοσυχνοτήτων. Παράλληλα, στην εργασία καταγράφονται οι απειλές που αντιμετωπίζουν τα ασύρματα δίκτυα στο φυσικό επίπεδο, όπως επιθέσεις υποκλοπών, παρεμβολών και τις μεθόδους ασφάλειας για την προστασία των δεδομένων και την αποτροπή κακόβουλων ενεργειών. Μέσα από την ανάλυση αυτή, η εργασία αποσκοπεί να προσφέρει μια ολοκληρωμένη εικόνα των ασύρματων δικτύων από την σκοπιά του φυσικού επιπέδου και την αξιοποίηση ενός πολύ χρήσιμου και πρακτικού εργαλείου.
description: The thesis focuses on the RF Propagation Calculator tool and offers a complete stepby-step guide to the use and functions of the software. The detailed manual is accompanied by a set of laboratory exercises on the RF Propagation Calculator tool, which are based on architectures and technologies of the Psysical Layer of Wireless Networks. The techniques and technologies used to achieve efficient and reliable communication over radio frequencies are also discussed. At the same time, the paper lists the threats faced by wireless networks at the physical layer such as eavesdropping attacks, jamming and the security methods to protect data and prevent malicious actions. Through this analysis, the paper aims to provide a comprehensive view of wireless networks from the physical layer perspective and the utilization of a very useful and practical tool.
Guide for an assessment of a SIS enabling environment
Creators: name: CAB International
name: ISRIC World Soil Information
Publisher: name: Gates Open Research
Year: 2024

description: This document is to be used alongside the framework for sustainable national soil information systems and is not a standalone document.

This document is a supporting resource for component 2 in the SIS framework and seeks to provide a guide for the different topics that could be included in an assessment of the enabling environment of a soil information system (SIS). The purpose of this information gathering activity is to understand what exists that can support the SIS, what might pose a challenge to the SIS and what is missing that the SIS project team could address. The topics and suggested questions are not an exhaustive list, and we recommend addressing the chosen topics through desk research and stakeholder interviews.
Supplementary Materials for: Phylogenomics, lineage diversification rates, and the evolution of diadromy in Clupeiformes (anchovies, herrings, sardines, and relatives)
Creators: name: Egan, Joshua
Publisher: name: Zenodo
Year: 2022
Subjects: state-dependent diversification
speciation
migration
allopatric
exon capture
Next-generation sequencing
divergence time
ecological opportunity

description: Migration independently evolved numerous times in animals, with a myriad of ecological and evolutionary implications. In fishes, perhaps the most extreme form of migration is diadromy, the migration between marine and freshwater environments. A key and longstanding question is how does diadromy influence lineage diversification rates? Many diadromous species travel long distances during migration, have large geographic ranges, and use isolated freshwater habitats, which may increase the likelihood of speciation. Alternatively, diadromy may reduce lineage diversification rates if migration facilitates gene flow, homogenizing populations and stymieing speciation. Clupeiformes (herrings, sardines, shads and anchovies) is a model clade for testing hypotheses about the evolution of diadromy because it includes an exceptionally high proportion of diadromous species and several independent evolutionary origins of diadromy. However, relationships among major clupeiform lineages remain unresolved with sparse (<50%) sampling of diadromous species, limiting the resolution of phylogenetically-informed statistical analyses. We assembled a phylogenomic dataset and used multi-species coalescent and concatenation approaches to generate the most comprehensive, highly-resolved clupeiform phylogeny to date, clarifying relationships among major clades of Clupeiformes, revealing taxa requiring revision, and identifying recalcitrant relationships needing further examination. Using this phylogeny as the evolutionary framework for downstream comparative analyses, we tested the hypothesis that diadromous lineages diversified faster than lineages restricted to either marine or freshwater habitats. We found that transitions to diadromy were more common than transitions from diadromy to non-diadromy. The largest lineage diversification rate increase in clupeiforms is associated with a transition to diadromy, but we uncovered little statistical support for categorically faster lineage diversification rates in diadromous versus non-diadromous fishes. We propose that diadromy may increase the potential for accelerated lineage diversification, particularly in species that migrate long distances, but this potential may only be realized in certain biogeographic contexts.
description: Funding provided by: National Science Foundation
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000001
Award Number:
title: (Typus) Arthropoda. Gliederfüssler. (Classe) Crustacea. Krebsthiere. (Ordnung) Decapoda. Zehnfüssige Krebse (Metamorphose).; [1890]
title: [Lehrtafel]
titleType: Subtitle
Creators: name: Leuckart, Rudolf
Publisher: name: Universitätsbibliothek der Ludwig-Maximilians-Universität München
Year: 2023

description: Vorlageform des Erscheinungsvermerks: Druck und Verlag von Theodor Fischer in Cassel. - Papier (4 Druckbögen) auf Leinwand mit Holzbestäbung
Destabilized Metal Hydrides for Thermal Management
Creators: name: Wong, Joshua Daniel
Publisher: name: Purdue University Graduate School
Year: 2025
Subjects: classificationCode: 400199
subject: Aerospace engineering not elsewhere classified

description: Many high temperature applications require thermal protection systems (TPS) to operate over long durations or increasing thermal loads. Metal hydrides provide an opportunity to serve as a TPS with improved energy density over current systems. Furthermore, metal hydride TPS are potentially reusable and produce hot hydrogen gas which can be used in other subsystems. This paper investigates using destabilized calcium hydride (CaH2) for thermal management.Destabilization of a metal hydride involves combining a metal hydride with an additive that reacts during desorption to form an intermetallic compound. This reaction has a lower enthalpy than the desorption of hydrogen from the neat metal hydride. The lower enthalpy allows the hydrogen to desorb out of the metal hydride at a lower temperature for a given equilibrium pressure.We destabilized the metal hydrides in order to test at lower temperatures than neat CaH2, reducing costs and the lead time for testing. To investigate metal hydrides for thermal management, we designed a test stand to measure the volume of hydrogen generation and temperature inside the metal hydride powder bed during desorption for up to 0.38 g of CaH2. To test larger quantities of metal hydrides we used a test stand providing heat fluxes from 200-400 W/cm2 for up to 30 g of CaH2. We designed new sample containers with reduced material and machining costs than the original design, increased strength, and improved heat transfer. The reduction in material allows for lower cost iteration on the incorporated fin design to improve heat transfer. We destabilized CaH2 using alumina, aluminum, silicon, and zinc.The data suggests water contamination of alumina is a challenge when using it to destabilize CaH2, requiring the use of alumina that has been stored in an inert environment. Additionally, large scale testing of CaH2-α-alumina results in ~18% hydrogen desorption, indicating challenges regarding thermal conductivity. Furthermore, the data suggests CaH2‑α‑alumina, CaH2‑aluminum, CaH2-silicon, and CaH2-zinc do not produce significant endotherms and are subsequently not effective as TPS due to the poor effective thermal conductivity of the metal hydride powder mixture. Small scale testing showed poor hydrogen desorption for CaH2 destabilized with α‑alumina, aluminum, and silicon with ~50% of the theoretical hydrogen desorption. Zinc showed a higher yield at ~70% desorption. However, further testing is required to confirm these results.
Identification of ZBTB18 as a novel colorectal tumor suppressor gene through genome-wide promoter hypermethylation analysis
Creators: name: S Bazzocco
name: H Dopeso
name: Á Martínez-Barriocanal
name: E Anguita
name: R Nieto
name: J Li
name: E García-Vidal
name: V Maggio
name: P Rodrigues
name: PG De Marcondes
name: S Schwartz
name: LA Aaltonen
name: A Sánchez
name: Mariadason, John
name: D Arango
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Background: Cancer initiation and progression are driven by genetic and epigenetic changes. Although genome/exome sequencing has significantly contributed to the characterization of the genetic driver alterations, further investigation is required to systematically identify cancer driver genes regulated by promoter hypermethylation. Results: Using genome-wide analysis of promoter methylation in 45 colorectal cancer cell lines, we found that higher overall methylation levels were associated with microsatellite instability (MSI), faster proliferation and absence of APC mutations. Because epigenetically silenced genes could represent important oncogenic drivers, we used mRNA expression profiling of colorectal cancer cell lines and primary tumors to identify a subset of 382 (3.9%) genes for which promoter methylation was negatively associated with gene expression. Remarkably, a significant enrichment in zinc finger proteins was observed, including the transcriptional repressor ZBTB18. Re-introduction of ZBTB18 in colon cancer cells significantly reduced proliferation in vitro and in a subcutaneous xenograft mouse model. Moreover, immunohistochemical analysis revealed that ZBTB18 is frequently lost or reduced in colorectal tumors, and reduced ZBTB18 expression was found to be associated with lymph node metastasis and shorter survival of patients with locally advanced colorectal cancer. Conclusions: We identified a set of 382 genes putatively silenced by promoter methylation in colorectal cancer that could significantly contribute to the oncogenic process. Moreover, as a proof of concept, we demonstrate that the epigenetically silenced gene ZBTB18 has tumor suppressor activity and is a novel prognostic marker for patients with locally advanced colorectal cancer.
Additional file 1 of Prioritising nano- and microparticles: identification of physicochemical properties relevant for toxicity to Raphidocelis subcapitata and Daphnia magna
Creators: name: Hund-Rinke, Kerstin
name: Broßell, Dirk
name: Eilebrecht, Sebastian
name: Schlich, Karsten
name: Schlinkert, Ruben
name: Steska, Tim
name: Wolf, Carmen
name: Kühnel, Dana
Publisher: name: figshare
Year: 2024
Subjects: subject: Biochemistry
subject: Space Science
subject: Medicine
subject: Cell Biology
subject: Neuroscience
subject: Pharmacology
subject: Environmental Sciences not elsewhere classified
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: FOS: Biological sciences
subject: Cancer
subject: Inorganic Chemistry
subject: FOS: Chemical sciences
subject: Plant Biology

description: Additional file 1: Figure S1. Principal component analysis (PCA) of materials. (A) PCA based on size, zeta-potential, solubility, and EC50 values in algae for releasing no or only nontoxic ions (named as stable) materials (red) and those releasing toxic ions (blue). (B) PCA of stable materials based on the values for size, zeta-potential, and EC50 in algae. Colouring indicates the morphology of the material. (C) Same as in (B) but for materials releasing toxic ions. Here, solubility was taken into account. Table S1 Material selection and underlying objective. Table S2 Physicochemical properties measured to characterise the advanced materials and the corresponding detection method. Table S3 Characterisation data for stable materials (additional data for fibres, see Table S5; used methods, see Table S2). Table S4 Characterisation data for materials that release toxic ions (additional data for fibres, see Table S5; used methods, see Table S2). Table S5 Size distributions for the fibres (lengths and diameters). Table S6 Inhibition of R. subcapitata growth determined for materials tested with concentrations differing by a spacing factor of 10. Table S7 Ag nano- and microparticles - ecotoxicity on algae and selected physicochemical properties sorted by producer and ecotoxicity (for ecotoxicities of the nanoGAP-materials, the effect at the highest test concentration with less than 100% is used). Table S8 Ecotoxicity of inert nano- and micromaterials with different morphologies towards D. magna. The physical–chemical properties of the materials are listed in Table S1. Table S9 Ecotoxicity and uptake of materials of complex composition compared to individual-component analogues in D. magna. The physical–chemical properties of the materials are listed in Table S1. Table S10 Enlarged images showing the examples of agglomeration behaviour for particles and R. subcapitata presented in Figure 2A.
The economic case for rewetting England’s patchy lowland peat
Creators: name: Behrendt, Karl
name: Huang, Iona
name: Casperd, Julia
name: Millington, Anthony
name: Natálio, Ana Morais
name: Symmons, Jackie
name: Mayne, Kate
name: Sarfo, Yaw
name: Erskine, Louise
name: Kirby, Scott
Publisher: name: Unknown
Year: 2025
Subjects: subject: Agricultural and Food Policy
subject: Crop Production/Industries
subject: Paludiculture
subject: Biodiversity Net Gain
subject: stochastic bioeconomic modelling

description: Peatland provides significant value to English food production and economy, with their drainage and use leading to extensive degradation and GHG emissions. Although some agri-environmental policies support paludiculture, there remains significant uncertainty as to the private and public benefit of adopting paludiculture by landholders, especially in landscapes where lowland peats are patchy. Using a case study approach, we define the private and social benefits of six landholders in the West Midlands of England adopting paludiculture or peatland restoration for biodiversity markets to minimise or reverse further lowland peat degradation. A stochastic bioeconomic modelling approach is applied which integrates land use alternatives, payments from agri-environmental policies, and monetisation of biodiversity net gains when lowland peats are restored for habitat gain. In addition to the substantial capital costs of rewetting a catchment, land use opportunity costs, and regulatory and compliance costs affects the minimum feasible scale of rewetting lowland peat. These present significant barriers to the adoption of paludiculture, and current agri-environmental policies and payments are unlikely to be conducive to the adoption of paludiculture in small scale non-contiguous lowland peat. This puts at risk extensive areas of fragmented lowland peat and may result in continued lowland peat degradation, agriculture’s contribution to GHG emissions and catchments not benefiting from the flood mitigation and biodiversity gain opportunities rewetting lowland peat provides. Monetisation of biodiversity net gains when restoring lowland peat habitats potentially offers the most economically beneficial pathway to protecting England’s patchy lowland peats.
Additional file 2 of Genetic control of tolerance to drought stress in soybean
Creators: name: Saleem, Aamir
name: Roldán-Ruiz, Isabel
name: Aper, Jonas
name: Muylle, Hilde
Publisher: name: figshare
Year: 2024
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: Plant Biology

description: Additional file 2.
What teachers learn from science and arts integration in a design-based learning framework: An Australian study
Creators: name: Lovejoy, Valerie
name: V Prain
name: C Musk
name: L Poljak
name: D Roberts
name: I Stewart
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Secondary teachers face a plethora of advice on why and how to integrate science with the arts for mutual benefit. Diverse rationales for this integration are matched by wide variation in recommended programs. However, there is limited research on what exactly teachers learn, or need to learn, over time beyond the life of any particular approach or topic, to succeed. In this paper we contribute insights into this issue by analysing, in the light of relevant literature, participant teachers’ learning after four years experience of a standalone, two-day annual program that aimed to integrate science and the arts in a whole-school approach. Our case study entailed interviews with the principal and key participant teachers, observational notes on program enactment, and participation in teacher planning and review meetings. We found that: (a) participant teachers perceived multiple benefits from this curricular innovation; (b) the integration was enabled by a design-based learning framework, and (c) diverse conditions were needed for this innovation to be sustained, with implications for other forms of program integration.
Application of metabolomics in pediatric asthma: Prediction, diagnosis and personalized treatment
Creators: name: Papamichael, Maria
name: Katsardis, C
name: Sarandi, E
name: Georgaki, S
name: Frima, ES
name: Varvarigou, A
name: Tsoukalas, D
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Asthma in children remains a significant public health challenge affecting 5–20% of children in Europe and is associated with increased morbidity and societal healthcare costs. The high varia-tion in asthma incidence among countries may be attributed to differences in genetic susceptibility and environmental factors. This respiratory disorder is described as a heterogeneous syndrome of multiple clinical manifestations (phenotypes) with varying degrees of severity and airway hyper-responsiveness, which is based on patient symptoms, lung function and response to pharmacotherapy. However, an accurate diagnosis is often difficult due to diversities in clinical presentation. Therefore, identifying early diagnostic biomarkers and improving the monitoring of airway dysfunction and inflammatory through non-invasive methods are key goals in successful pediatric asthma manage-ment. Given that asthma is caused by the interaction between genes and environmental factors, an emerging approach, metabolomics—the systematic analysis of small molecules—can provide more insight into asthma pathophysiological mechanisms, enable the identification of early biomarkers and targeted personalized therapies, thus reducing disease burden and societal cost. The purpose of this review is to present evidence on the utility of metabolomics in pediatric asthma through the analysis of intermediate metabolites of biochemical pathways that involve carbohydrates, amino acids, lipids, organic acids and nucleotides and discuss their potential application in clinical practice. Also, current challenges on the integration of metabolomics in pediatric asthma management and needed next steps are critically discussed.
Efficient bioflocculation of chlorella vulgaris with a chitosan and walnut protein extract
Creators: name: K Xu
name: X Zou
name: A Mouradov
name: Spangenberg, German
name: W Chang
name: Y Li
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Bioflocculation represents an attractive technology for harvesting microalgae with the potential additive effect of flocculants on the production of added-value chemicals. Chitosan, as a cationic polyelectrolyte, is widely used as a non-toxic, biodegradable bioflocculant for many algal species. The high cost of chitosan makes its large-scale application economically challenging, which triggered research on reducing its amount using co-flocculation with other components. In our study, chitosan alone at a concentration 10 mg/L showed up to an 89% flocculation efficiency for Chlorella vulgaris. Walnut protein extract (WPE) alone showed a modest level (up to 40%) of flocculation efficiency. The presence of WPE increased chitosan’s flocculation efficiency up to 98% at a reduced concentration of chitosan (6 mg/L). Assessment of co-flocculation efficiency at a broad region of pH showed the maximum harvesting efficiency at a neutral pH. Fourier transform infrared spectroscopy, floc size analysis, and microscopy suggested that the dual flocculation with chitosan and walnut protein is a result of the chemical interaction between the components that form a web-like structure, enhancing the bridging and sweeping ability of chitosan. Co-flocculation of chitosan with walnut protein extract, a low-value leftover from walnut oil production, represents an efficient and relatively cheap system for microalgal harvesting.