Additional file 1 of AGA induces sub-G1 cell cycle arrest and apoptosis in human colon cancer cells through p53-independent/p53-dependent pathway
Creators: name: Peng, Bou-Yue
name: Singh, Abhinay Kumar
name: Chan, Chun-Hao
name: Deng, Yue-Hua
name: Li, Pin-Ying
name: Su, Chun-Wei
name: Wu, Chia-Yu
name: Deng, Win-Ping
Publisher: name: figshare
Year: 2023
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Molecular Biology
subject: Physiology
subject: Pharmacology
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Cancer
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: 60506 Virology

description: Additional file 1: Supplementary figure 1. Raw images of western blot (including blot images as they are, with the membrane edges and all repeats) represent the AGA (0, 10, and 20 mg) extract effects on three different cell lines SW620, SW480, and HT29 to investigate expression of cell cycle proteins. (A) Represent the cdk4, (B) cdk2, (C) p21, and (D) cdk6 protein expression after AGA extract effect on three colon cancer cell lines. All data were represented with their three repeats; and red colour brackets in original blot images indicates edges of membrane in each images that used in manuscript of figure 4ABC. Supplementary figure 2. Raw images of western blot (including blot images as they are, with the membrane edges and all repeats) represent the AGA (0, 10, and 20 mg) extract effects on three different cell lines SW620, SW480, and HT29 to investigate expression of cell cycle proteins. (A) Represent the cdk4, (B) cdk2, (C) p21, and (D) cdk6 protein expression after AGA extract effect on three colon cancer cell lines. All data were represented with their three repeats; and red colour boxes in original blot images indicates that images are used in manuscript of figure 4ABC. Supplementary figure 3. Raw images of western blot (including blot images as they are, with the membrane edges and all repeats) represent the AGA (0, 10, and 20 mg) extract effects on three different cell lines SW620, SW480, and HT29 to investigate expression of apoptosis. (A) Represent the p53, (B) Bax, and (C) Caspase 9 protein expression after AGA extract effect on three colon cancer cell lines. All data were represented with their three repeats; and red colour brackets in original blot images indicates edges of membrane in each images that used in manuscript of figure 6ABC. Supplementary figure 4. Raw images of western blot (including blot images that used in manuscript) represent the AGA (0, 10, and 20 mg) extract effects on three different cell lines SW620, SW480, and HT29 to investigate expression of apoptosis. (A) Represent the p53, (B) Bax (in bax blot SW480 mention as 20, 10, 0, but in manuscript used as 0, 10, 20), and (C) Caspase 9 protein expression after AGA extract effect on three colon cancer cell lines. All data were represented with their three repeats; and red colour boxes in original blot images indicates that images are used in manuscript of figure 6ABC. Supplementary figure 5. Raw images of western blot (including blot images as they are, with the membrane edges and all repeats) represent the AGA (0, 10, and 20 mg) extract effects on three different cell lines SW620, SW480, and HT29 to investigate expression of B-actin (A) Represent the original blot images of B-actin with repeats; red colour brackets in original blot images indicates edges of membrane in each images that used in manuscript of figure 4ABC and figure 6ABC. (B) Original blot images of B-actin with repeats, red colour boxes in original blot images indicates that images are used in manuscript of 4ABC and figure 6ABC.