cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Hepatocyte Nox proteins in the pathogenesis of hepatocellular carcinoma
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Hepatitis C virus (HCV) is a major etiologic agent of hepatocellular carcinoma (HCC) that is estimated to have infected 170 million people worldwide. HCV-induced HCC is typically preceded by cirrhosis but the mechanism by which HCV induces HCC is still unclear. HCV elevates the levels of 8-hydroxydeoxyguanosine, a marker of oxidative DNA damage, and double strand DNA breaks. These alterations to the DNA increase the likelihood of mutations to important cell regulatory genes in the development of cancer. In terms of the mechanism, HCV increases the production of superoxide, hydrogen peroxide, and nitric oxide that can generate hydroxyl radical and peroxynitrite, which can damage the DNA. However, the source of superoxide and hydrogen peroxide during HCV infection remains incompletely characterized. Recently, hepatocytes have been found to express NAD(P)H oxidase (Nox) family proteins. Nox family proteins consist of Nox and Duox proteins that transfer electrons from NAD(P)H to O2 to generate reactive oxygen species (ROS). Preliminary studies in my laboratory show persistent elevation of Nox4 and Nox1 mRNA's in Huh7 human hepatoma cells with HCV. Nox4 and Nox1 protein levels were also increased in HCV-transfected and HCV-infected Huh7 cells as well as HCV-infected human liver. In addition, HCV-induced a pro-oxidative state in Huh7 human hepatoma cells that was sensitive to diphenylene iodonium, an inhibitor of flavoenzymes including Nox enzymes. Unlike Nox1, which showed cytosolic, extranuclear localization, Nox4 was partly nuclear in these cells, and its nuclear localization increased with HCV. The goal of this study, therefore, is to determine the role of hepatocyte Nox proteins in HCV-induced oxidative DNA damage. Virus producing cell culture models, other well-established in vitro HCV models, Nox-over-expressing cells, and HCV-infected and –uninfected human liver samples will be used. We hypothesize that HCV activates hepatocyte Nox proteins and that these proteins serve as robust, regulated sources of ROS for oxidative DNA damage during HCV infection. Nox4 is also known to be induced by transforming growth factor beta and tumor necrosis factor alpha, which are fibrogenic and inflammatory cytokines, and may thus provide a link between HCV infection, chronic inflammation, fibrogenesis, and development of HCC. The long-term goal is to understand the role of hepatic Nox proteins and oxidative stress in liver cancer for the development of novel therapy, directed at the source of ROS
Trichomanes boivinii Bosch
Creators: name: Ahamada H. Saïd
name: Sabine Hennequin
name: Germinal Rouhan
name: Jean-Yves Dubuisson
Publisher: name: Zenodo
Year: 2017
Subjects: Biodiversity
Taxonomy
Plantae
Tracheophyta
Polypodiopsida
Hymenophyllales
Hymenophyllaceae
Trichomanes
Trichomanes boivinii

description: Trichomanes boivinii Bosch
MADAGASCAR: Réserve Intégrale de Lokobe, 27 Jul. 1992, F.Rakotondrainibe 1788 (P00100302); Péninsule de Masoala, 20 Oct. 2004, G.Rouhan 326 (P00749272).
description: Published as part of Ahamada H. Saïd, Sabine Hennequin, Germinal Rouhan & Jean-Yves Dubuisson, 2017, Disentangling the diversity and taxonomy of Hymenophyllaceae (Hymenophyllales, Polypodiidae) in the Comoros, pp. 1-53 in European Journal of Taxonomy 313 on page 53, DOI: 10.5852/ejt.2017.313, http://zenodo.org/record/896401
Total synthesis of welwistatin, a potent anticancer agent
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: This proposal outlines the total synthesis of welwistatin, a marine natural product that was isolated in 1994. Studies indicate that welwistatin exhibits the remarkable ability to reverse MDR to a variety of anti-cancer drugs. The attractive biological profile of welwistatin, coupled with its intricate structure, has aroused the interest of synthetic chemists worldwide. Despite the efforts of several research groups, a laboratory synthesis of welwistatin remains an elusive goal. Our approach to the synthesis of welwistatin involves the generation of highly reactive aryne derivatives of indoles, or indolynes, which function as electrophilic indole surrogates. Preliminary results have shown that these intermediates can be accessed under fairly mild conditions, which would likely be amenable to the synthesis of complex molecules. We describe herein a novel route to welwistatin that could afford the natural product in less than 15 steps. Given the concise and convergent nature of our approach, the synthesis of welwistatin analogs should also be feasible, which would enable advanced biological studies. Ultimately, our efforts could lead to the discovery of new therapeutics for the treatment of cancers that display multiple drug resistance.
Identification of glioma susceptibility genes in the dog
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Gliomas are central nervous system tumors that are highly aggressive. They are ultimately lethal in people. Genetics plays a key role in these tumors from the standpoint of susceptibility loci as well as disease progression. Studies that reveal genes involved with either of these two processes have the potential to provide new avenues of treatment. The dog is an exceptional model for investigating gliomas since these brain tumors are naturally occurring in this species. In addition, certain breeds are predisposed to form gliomas more than others, indicating a genetic etiology in dogs. Dog breeds with brachycephalic head morphology are more commonly diagnosed with gliomas than other breeds. However, not all brachycephalic dog breeds appear to be susceptible. Therefore, the primary locus for brachycephaly is not the cause of glioma susceptibility, although it may be a contributing factor. We have taken a novel mapping approach in the dog to identify a strong genetic association to the primary locus for brachycephaly. We propose to take a similar approach that utilizes a canine whole genome array to identify genetic associations with glioma susceptibility in the dog.
Experimental assessment of laser-assisted cryosurgery: Ex-vivo and in-vivo studies
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Current research in cancer cryosurgery is concerned with developing a practical therapeutic procedure that will absolutely destroy cancerous tumors while minimizing damage to the surrounding healthy tissue. The goal of this project is therefore to assess the feasibility of a novel cryosurgical procedure we refer to as Laser-Assisted Cryosurgery (LAC), where controlled laser irradiation is used to induce volumetric heat generation around the target tumor. Combined properly with tumor cryofreezing, this approach better shapes and confines the frozen region to strict boundaries, within which tumor necrosis is ensured and beyond which viable and healthy tissue prevails. Theoretical and preliminary ex vivo experimental work have already proven that LAC is a novel treatment modality that overcomes the limitations of traditional cryosurgery, such as the need to predefine freezing and thawing rates and the necessity to monitor with strict accuracy the advancement of the frozen tissue. We expect that the LAC procedure that will be developed in the present proposal will be especially suitable for solid tumors, and may provide unique advantages to patients with hepatocellular carcinoma (HCC) and prostate cancer. The precision of LAC cryoablation is particularly valuable in HCC cases to minimize damage to nontumorous tissue while preserving liver function. Also, for prostate cancer cryosurgery, LAC is expected to provide effective cancer control while preserving sexual potency and urinary continence. The specific aims for this 1-year study are: (1) Perform temperature measurements during LAC procedures on ex vivo mice hepatic tissue using mice transfected with green fluorescent protein (GFP) and correlate the temperature histories with postoperative viability assays, and (2) Perform identical LAC procedures on in vivo mice hepatic tissue and quantify the differences between ex vivo and in vivo experiments in terms of the thickness of the protected region to establish the in vivo effects on LAC. The use of GFP as a tissue viability marker is a NOVEL application in the field of cryobiology, laser tissue interaction and cryosurgery. The advantages of this technique are that the treated tissues do not require to be perfused with dyes in order to demarcate viable from non-viable tissue, avoiding any postprocessing of the tissue samples before they can be evaluated.
Microtubule acetylation and cancer
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Microtubules (MTs) are dynamics structures that are predominantly nucleated at the centrosome and the Golgi apparatus. They are critical for efficient intracellular transport and organelle positioning during interphase and for spindle formation during mitosis. To accomplish these diverse roles, MTs undergo numerous posttranslational modifications, whose functions are only poorly understood. MT acetylation, a conserved modification which has been proposed to stabilize MT, to regulate cell motility and to enhance intracellular transport. Several studies have also shown that enhancing MT acetylation may be beneficial in cancer treatment. However, further investigations are necessary as it has been difficult to isolate the specific contribution of acetylated MTs to the observed phenotypes. We have identified unique experimental conditions in which the majority of centrosome/Golginucleated acetylated MTs are abolished. We found that depletion of the Golgi proteins GRASP65 and GM130 resulted in a loss of acetylated MTs. While both proteins are in a complex with acetylated MTs, MT acetylation appears to depend on the Golgi association of GRASP65, which is controlled by GM130. We wish to understand how GRASP65 controls MT acetylation and how the loss of acetylated MTs affects cell motility and the invasion potential of cancer cells. Aim 1: Determine the range of proteins whose acetylation is regulated by GRASP65. We will compare the effect of GRASP65 depletion on acetylated MTs that are nucleated at the centrosome and Golgi apparatus vs primary cilia. We will also test if GRASP65 is required for protein acetylation in general or only for the specific modification of tubulin. Aim 2: Understand how a loss of Golgi-bound GRASP65 results in a loss of acetylated MTs. We will investigate whether the loss of acetylated MTs is due to changes in the stability of acetylated MTs or in an alteration in their modification rate. We will also examine if the function of GRASP65 in MT acetylation is dependent on its association with GM130 and acetylated MTs. Aim 3: Examine the link between GRASP65 function in MT acetylation and oncogenesis. We will examine whether the levels of GRASP65 and GM130 are altered in 60 different cancer cell lines using Western blot analysis. We will also test whether changes in GRASP65 levels alter cell motility in 2D migration assays and 3D invasion assays. The results from this study will show whether MT acetylation may serve as suitable target for anticancer treatments.
Screens for novel regulators of the TOR complex 2 signaling pathway
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: The cellular homolog of the v-akt oncogene, Akt/protein kinase B (PKB), plays pivotal roles in cell survival, proliferation and growth that drive tumor progression. Akt is activated in response to insulin and growth factors that induce activation of phosphatidylinositol-3-kinase and generation of phosphatidylinositol-3,4,5-trisphosphate. Full activation of Akt requires two phosphorylation events, one in its T-loop (activation loop) and the other in its C-terminal hydrophobic motif. The Tloop phosphorylation is carried out by PDK1 (phosphoinositide dependent kinase 1), while the phosphorylation of the hydrophobic motif is carried out by TOR complex 2 (TORC2). TORC2 is an evolutionarily conserved protein complex of the TOR (target of rapamycin) protein kinase with regulatory subunits including rictor, mSIN1 and mLST8. However, very little is known about the molecular mechanism that regulates TORC2 activity. The proposed research aims to discover the regulatory mechanisms that modulate the phosphorylation of the TORC2- and PDK1-dependent phosphorylation of Akt, using the fission yeast Schizosaccharomyces pombe as a model system. Our laboratory has recently demonstrated that the TORC2–Akt signaling pathway is conserved in S. pombe as the TORC2–Gad8 pathway. Like human Akt, S. pombe Gad8 kinase is regulated by two phosphorylation sites in its C-terminal hydrophobic motif and T-loop, which are phosphorylated by TORC2 and Ksg1, a S. pombe ortholog of PDK1. Using the recently developed antibodies that specifically recognize these phosphorylation sites in Gad8, we will conduct genome-wide screens, aiming to identify genes that regulate TORC2 and Ksg1 in the phosphorylation and activation of Gad8. We anticipate that novel regulators identified in these screens will extend our understanding of the mechanism that controls the evolutionarily conserved Akt pathway.
Elucidation of a potential role of a histone methyltransferase subunit in coordinating TGN traffic and gene expression
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Tumorgenesis is an orchestrated process of multiple cellular events. Two such events, aberrant histone lysine methylation/gene expression and altered trafficking of lysosomal enzymes have been independently observed during the development of multiple types of cancers. However, the molecular mechanism for this altered trafficking behavior of lysosomal enzymes remains elusive. It is also unknown whether a link exists between targeting of lysosomal enzymes and histone lysine methylation/gene expression. We have discovered that one subunit of a histone 3 lysine 4 methyltransferase complex, mammalian homolog of Caenorhabditis elegans Dpy-30 protein (mDpy-30), resides in both the nucleus and the trans-Golgi network (TGN). Based on our preliminary data, we hypothesize that the nuclear pool of mDpy-30 regulates transcription of cation-dependent mannose 6-phosphate receptor (CD-MPR), a cargo receptor involved in packaging newly synthesized lysosomal enzymes into transport carriers at the TGN. The TGN pool of mDpy-30 controls trafficking of CD-MPR and lysosomal enzymes by regulating the activity of ADP-ribosylation factor 1 (ARF1), a key GTPase controlling the formation of transport carriers destined to endosomes/lysosomes. If verified, mDpy-30 may modulate the lysosomal function during tumorgenesis by controlling both trafficking and expression of CD-MPR. To test our hypothesis, we propose the following specific aims: (1) We intend to adopt both biochemical and imaging approaches to test the hypothesis that mDpy-30 modulates the ARF1 activity and/or CD-MPR trafficking at the TGN. (2) We plan to utilize chromatin immunoprecipitation analysis to test the hypothesis that mDpy-30 regulates transcription of CD-MPR gene in the nucleus via the histone methyltransferase complex. Since CD-MPR is a major receptor involved in the recognition and delivery of lysosomal enzymes from TGN to endosomes/lysosomes and ARF1 is a key regulator of TGN-to-endosome/lysosome trafficking, the verification of our hypothesis will raise the intriguing possibility that the dual roles of mDpy-30 allow it to modulate the secretion of lysosomal enzymes during tumorgenesis at the levels of membrane trafficking and gene expression, and thus open new ground in cancer research.
Susceptibility to ovarian cancer is related to biotransformation capacity
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Ovarian cancer is the most lethal gynecological malignancy, yet the etiology of ovarian cancers is poorly understood. Depletion of ovarian follicles in mice by administration of ovotoxic chemicals like polycyclic aromatic hydrocarbons (PAHs) leads to the development of ovarian cancers. Tobacco smoke contains polycyclic aromatic hydrocarbons, and women who smoke may be at increased risk of ovarian cancer. The metabolism of PAHs involves enzymes regulated by the Cap- N-Collar basic leucine zipper transcription factor Nrf2. Nrf2 mice have decreased constitutive and inducible levels of several antioxidant and detoxifying enzymes involved in PAH metabolism. 90% of malignant ovarian cancers are thought to arise from ovarian surface epithelial cells, but the effects of PAHs on these cells have not been studied. We hypothesize that decreased capacity to detoxify PAHs in mice that lack Nrf2 increases susceptibility to ovarian surface epithelial cell DNA damage, malignant transformation, and, ultimately, ovarian cancer. We will use ovarian surface epithelial cells from Nrf2 and wild type mice to directly test the role of Nrf2 in events leading to malignant transformation by benzo[a]pyrene in the following Specific Aims: Specific Aim 1) To quantify effects of the PAH benzo(a)pyrene on proliferation and apoptosis in ovarian surface epithelial cells from Nrf2 compared to wild type mice. Specific Aim 2) To quantify reactive oxygen species, using laser scanning confocal microscopy with fluorescent probes, and DNA adducts, using 32P-postlabeling, single cell gel electrophoresis, and immunohistochemical methods, after treatment of Nrf2 and wild type ovarian surface epithelial cells with benzo(a)pyrene, and to test protective effects of antioxidants. Specific Aim 3) To compare the sensitivity of ovarian surface epithelial cells from Nrf2 and wild type mice to malignant transformation by benzo(a)pyrene. These studies, by elucidating the roles that reactive oxygen species and antioxidant and biotransformation enzymes play in the malignant transformation of ovarian surface epithelial cells, will contribute to the larger goals of identifying new targets for prevention and early diagnosis of ovarian cancer.
Vandenboschia gigantea (Bory ex Willd.) Pic.Serm.
Creators: name: Ahamada H. Saïd
name: Sabine Hennequin
name: Germinal Rouhan
name: Jean-Yves Dubuisson
Publisher: name: Zenodo
Year: 2017
Subjects: Biodiversity
Taxonomy
Plantae
Tracheophyta
Polypodiopsida
Hymenophyllales
Hymenophyllaceae
Vandenboschia
Vandenboschia gigantea

description: Vandenboschia gigantea (Bory ex Willd.) Pic.Serm.
MADAGASCAR:; Massif du Manongarivo, 29 Sep. 2004, T.Janssen et al. 2417 (P00590653); Massif du Tsaratanana, 7 May 2005, T.Janssen 2858 (P00590910).
LA RÉUNION: Forêt de Mare Longue, 22 Apr. 2002, J.Y.Dubuisson HR 2002­14 (P02433296).
description: Published as part of Ahamada H. Saïd, Sabine Hennequin, Germinal Rouhan & Jean-Yves Dubuisson, 2017, Disentangling the diversity and taxonomy of Hymenophyllaceae (Hymenophyllales, Polypodiidae) in the Comoros, pp. 1-53 in European Journal of Taxonomy 313 on page 53, DOI: 10.5852/ejt.2017.313, http://zenodo.org/record/896401
Measurement of the target single-spin asymmetry in quasi-elastic region from the reaction ³He↑(e, e̷̷̷̷)
Creators: name: Zhang, Yawei
Publisher: name: No Publisher Supplied
Year: 2013

description: A measurement of the inclusive target single-spin asymmetry, A[subscript y] ³He, has been performed using the quasi-elastic ³He↑(e, e̷̷̷̷) reaction with a vertically polarized  ³He target at Q̷̷̷̷² values of 0.13, 0.46 and 0.9& GeV̷̷̷². This asymmetry vanishes under the one photon exchange assumption. But the interference between two-photon exchange and one-photon exchange gives rise to an imaginary amplitude, so that a non-zero A[subscript y] is allowed. The experiment, conducted in Hall A of Jefferson Laboratory in 2009, used two independent spectrometers to simultaneously measure the target single-spin asymmetry,   A[subscript y] ³He.  Using the effective polarization approximation, the neutron single-spin asymmetries, $A[superscript n, subcript y], were extracted from the measured  ³He asymmetries. The measurement is the first to firmly establish a non-vanishing A[subscript y]. Non-zero asymmetries were observed at all Q̷̷̷̷² points, and the final overall precision is an order of magnitude improved over the existing proton data. The data provide new constraints on Generalized Parton Distribution (GPD) models and new information on the dynamics of the two-photon exchange process.
Awaous banana
Creators: name: Angulo, Arturo
name: Betts, Joel T.
name: González-Alemán, Néstor J.
name: Castañeda, Edgar
name: Berghe, Eric Van Den
name: Elías, Diego J.
name: Mcmahan, Caleb D.
name: Matamoros, Wilfredo A.
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Chordata
Perciformes
Gobiidae
Awaous
Awaous banana

description: Awaous banana (Valenciennes 1837). Atlantic River Goby; Chupapiedras, Guavina, Lamearenas, Gobio, Gobio de Río del Atlántico, Dormilón
Vouchers: UCR 0457-013 (1), 0458-008 (8), 0460-004 (3), 0461-008 (12), 0462-005 (4), 1066-002 (13) and 3225- 004 (1); LSUMZ 15393 (2) and 17156 (2); other collections: UF 106971 (1). Distribution: North, Central and South America; from northern Florida, USA, southward through the Greater and Lesser Antilles to Trinidad and Tobago and Venezuela, Atlantic drainages; (Nicaragua) Co, UI, WK, Pr, GM, KP, Es, BF, IM, To, Sa, SJ, RF and LN (Atlantic); 0–681 masl; Per, Dia, Cat. Occurrence and conservation status: Nat; LC (2019), population trend stable. Literature: Villa (1982: 226, as Awaous tajasica (Lichtenstein 1822) —misidentification; detailed description, including illustrations and an identification key, and information on distribution and ecology), Bussing (1998: 396; brief description, including illustrations and an identification key, and information on distribution, with a map, and ecology), González-Alemán (2006: 73; brief description, including an illustration, and information on distribution and ecology), Gros & Miguel-Frithz (2010: 181; brief description, including illustrations, and information on distribution and ecology, plus indigenous science and ethnoichthyology), van den Berghe (2015: 37; listed, including an illustration and information on distribution and ecology), Robertson & Van Tassell (2019: 4479; detailed description, including illustrations, and information on distribution, with a map, and ecology), Angulo (2021: 36; listed, including information on distribution) and McMahan et al. (2021: 970; phylogenetic relationships, and information on distribution, with a map, and ecology).
description: Published as part of Angulo, Arturo, Betts, Joel T., González-Alemán, Néstor J., Castañeda, Edgar, Berghe, Eric Van Den, Elías, Diego J., Mcmahan, Caleb D. & Matamoros, Wilfredo A., 2023, Continental fishes of Nicaragua: diversity, distribution and conservation status; with an annotated and illustrated checklist of species and an identification guide to families, pp. 1-89 in Zootaxa 5376 (1) on page 35, DOI: 10.11646/zootaxa.5376.1.1, http://zenodo.org/record/10208788
MOESM1 of Antimicrobial and antioxidant potential of different solvent extracts of the medicinal plant Geum urbanum L.
Creators: name: Dimitrova, Lyudmila
name: Zaharieva, Maya
name: Popova, Milena
name: Kostadinova, Nedelina
name: Tsvetkova, Iva
name: Vassya Bankova
name: Najdenski, Hristo
Publisher: name: Figshare
Year: 2017
Subjects: subject: Biophysics
subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Pharmacology
subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: Hematology
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Additional file 1: Figure S1. 1H and 13C NMR spectra of tormentic acid (1) in Pyridine d5. Figure S2. 1H, 13C, HSQC and HMBC NMR spectra of 3-O-methylellagic acid-3′-O-α-3″-O-acetylrhamnopyranoside (2) in CDCl3:CD3OD 1:1. Figure S3. 1H NMR spectrum of 3-O-methylellagic acid-3′-O-α-2″-O-acetylrhamnopyranoside (3) in CDCl3:CD3OD 1:1. Figure S4. 1H NMR spectrum of cathechin (4) in CD3OD. Figure S5. 1H, HSQC and HMBC NMR spectra of 3,3′-di-O-methylellagic acid-4-O-β-d-glucopyranoside (5) in DMSO. Figure S6. 1H and 13C NMR spectra of niga-ichigoside F1 (6) in CD3OD. Figure S7. 1H spectrum of gein (7) in CD3OD.
CHIPP Community Roadmap 2024. Update of Swiss Particle Physics Community Needs for Research Infrastructures 2029-2032 and beyond
Creators: name: Kilminster, B
name: Crivelli, P
name: Dissertori, G
name: Golling, T
name: Isidori, G
name: Montaruli, T
name: Müller, K
name: Seidel, M
name: Sgalaberna, D
name: Spira, M
name: Weber, M
name: Benelli, A
Publisher: name: Swiss Academies of Arts and Sciences
Year: 2024

description: This document is an update to the ‘CHIPP Roadmap for Research and Infrastructure 2025-2028 and beyond’ that was published in 2021. It presents the needs of the Swiss particle and astroparticle physics community in terms of future national and international research infrastructures. The content of the previous roadmap can serve as a reference and provides detailed context for the updates within this document.

 

Together with similar community roadmaps in other disciplines, it is an element of the four-year process leading to the development of the Swiss Roadmap for Research Infrastructures 2027 to be written by the State Secretariat for Education, Research and Innovation (SERI) in view of the ERI Dispatch 2029–2032 to Federal Council. The role of these ‘bottom-up’ inputs is to serve as an important basis for the strategic planning of the higher education institutions on new or major upgrades to national infrastructures and to inform and support SERI during its decision-making process on Swiss participation in international research infrastructure networks and organisations.
Report of the Union County Mosquito Extermination Commission for 1940
Creators: name: Collins, Roi C.
Publisher: name: No Publisher Supplied
Year: 1940

description: A yearly series of reports from the Union County Mosquito Extermination Commission. The reports cover finances, inspections, drainage, oiling, and salt marsh, inland, and educational work regarding the extermination and control of mosquitoes in the different towns and districts of Union County, New Jersey.
Chromatin insulators in human cells
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Chromosomes are divided into chromatin domains and transcriptionally inactive heterochromatic domains often reside adjacent to active euchromatic domains. DNA elements that separate domains are called insulators. There are two classes of insulators- enhancer blockers and silencer blocking barrier elements. Insulators are integral to proper gene regulation and mutations in insulators have been shown to result in disease states. They are also being used in numerous gene therapy vectors suggesting commercial potential. Numerous insulators have been identified in different organisms but most insulators identified to date are enhancer blockers and contain binding sites for RNA pol II transcription factors. Recent studies from several different groups show that in yeast and mice, tRNA genes and pol III transcription factors function as robust insulators. We intend to study pol III transcribed insulators in human cells in order to better understand their mechanism of function and to exploit these elements in research and medicine. Our analyses of the sequenced human genome demonstrate that tRNA genes cluster at specific sites on the chromosomes. One such cluster flanks the cell cycle regulated AurkB gene. We wish to determine if these clustered pol III transcribed genes function as insulators in human cells. Using specific enhancer-blocking and barrier assays we will initially analyze human pol III transcribed genes for insulator function. Sites identified as putative insulators using these assays will be further characterized at their native loci in human cells. Insulators normally have been shown to map to the boundaries of chromatin domains. We will determine if the putative pol III transcribed insulators map to the boundaries of the AurkB chromatin domain by chromatin immunoprecipitation analyses. The functional consequences of the inactivation of the insulator will also be investigated. Identifying novel pol III transcribed insulators will increase our molecular toolkit for research and medicine.
Metabolite-dependent cell cycle arrest in cancer cells
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Development of effective treatment strategies for cancer depends on identification of conditions that specifically kill cancer cells but do not affect normal cells. We have recently extended previous work and demonstrated that most cancer cells cannot survive in growth media where the amino acid methionine is replaced with its immediate metabolic precursor homocysteine. Importantly non-cancer cells are indifferent to replacement of methionine with homocysteine. This unique metabolic requirement of cancer cells presents a promising new opportunity for novel approaches in cancer therapy. However, replacing methionine with homocysteine in patients is not feasible. Therefore, to exploit the methionine/homocysteine effect for cancer treatment, it will be important to generate a molecular understanding of how replacing methionine with homocysteine specifically blocks cancer cell proliferation while having no effect on normal cells. However, despite the dramatic effects of the methionine/homocysteine replacement on cancer cell survival there is currently a complete lack of molecular understanding. We propose two specific aims that are independent from each other and try to generate a strong set of preliminary data that will allow us to prepare a competitive proposal for major extramural funding. (1) Develop initial molecular understanding of the cell cycle events that are triggered by the methionine/homocysteine effect. Experiments will analyze cell cycle progression/apoptosis and follow abundance and/or activity of key regulators in these pathways. (2) Measure changes and test re-addition effects of key metabolites in association with the methionine/homocysteine effect. These studies will use mass spectrometric approaches to determine intracellular changes of selected metabolites in the relevant metabolic pathway.
Upconversion nanoparticles for photodynamic cancer therapy
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Photodynamic therapy (PDT), in which a photosensitizing drug (photosensitizer) is activated by specific wavelengths of light to produce cytotoxic oxygen species and kill nearby malignant cells, is a revolutionary medical technology that provides an alternative form of cancer therapy in a non-surgical, minimally-invasive way. However, the light needed to activate most photosensitizers is usually in the visible range, which significantly limits the penetration depth of the treatment. Significant efforts have been devoted to the development of photosensitizers that specifically target cancer cells, and are activated by light that can penetrate deeper or larger tumors. Energy upconversion materials can efficiently convert low energy photons (e.g. IR) into higher energy photons (e.g. visible). Exploiting this unique property, we propose a new type of Multifunctional Upconversion NanoParticles (MUNPs) that simultaneously addresses two critical challenges in (PDT): effective penetration depth and specific targeting. The hypothesis is that by conjugating energy upconversion nanoparticles with available photosensitizers and specific molecular probes we create a platform in which the nanoparticle core absorb IR irradiation and emit visible light which will then activate the photo sensitizers to kill the tumor cells. In the mean time, the molecular probe will guide the specific targeting of cancer cells. We will target the MUNP to breast cancer cell markers to test our hypothesis. We have synthesized Yb, Er or Tm codoped NaYF4 nanoparticles (~10nm) with well controlled morphology and composition. By adjusting the dopant ratios, we are able to tune the emission wavelengths (blue, green and red) of the nanoparticles with absorption in near IR (980nm). Subsequently, we will conjugate the NPs to photosensitizers whose activation wavelength overlapping the emission of the nanoparticles. To explore this material system for energy upconversion process mediated PDT. We will couple our nanoparticles to recently developed small antibody fragments (Cys-diabodies) that bind to breast cancer markers (Her2 receptors), and characterize their uptake by BT474 cells in vitro. BT474 cells will be incubated with varying concentrations of the MUNP. Uptake and PDT efficacy will be assessed by confocal microscopy and cell viability studies. In addition, MUNPs will be radiolabeled and subject to biodistribution studies in mouse using microPET. Her2 positive and negative xenografts will be established in mouse mo
Awaous transandeanus
Creators: name: Angulo, Arturo
name: Betts, Joel T.
name: González-Alemán, Néstor J.
name: Castañeda, Edgar
name: Berghe, Eric Van Den
name: Elías, Diego J.
name: Mcmahan, Caleb D.
name: Matamoros, Wilfredo A.
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Chordata
Perciformes
Gobiidae
Awaous
Awaous transandeanus

description: Awaous cf. transandeanus (Günther 1861). Pacific River Goby; Chupapiedras, Guavina, Lamearenas, Gobio, Gobio de Río del Pacífico, Dormilón
Vouchers: UCR 0052-009 (1), 0183-004 (2), 0184-003 (30) and 0270-005 (23). Distribution: North, Central and South America; from central Baja California Sur and Sonora, Mexico, to Tumbes, Peru, Pacific drainages; (Nicaragua) Ch, Ne, ER, PN, Ni (Pacific); 0–319 masl; Per, Dia, Cat. Occurrence and conservation status: Nat; LC (2020), population trend unknown. Literature: Villa (1982: 227; detailed description, including illustrations and an identification key, and information on distribution and ecology), Carr & Giovannoli (1994: 111, as A. tajasica —misidentification; brief description, including information on distribution and ecology), Bussing (1998: 398; brief description, including illustrations and an identification key, and information on distribution, with a map, and ecology), Angulo (2021: 36; listed, including information on distribution) and McMahan et al. (2021: 970; phylogenetic relationships, and information on distribution, with a map, and ecology). Remarks: Based on molecular data, McMahan et al. (2021) suggested the presence of two different lineages under the name A. transandeanus in the Pacific coast of Mexico and Central America. The boundary between the two lineages is the northern portion of the Costa Rican Pacific (Guanacaste) coast. Morphological studies are necessary to corroborate this and to properly diagnose both species.
description: Published as part of Angulo, Arturo, Betts, Joel T., González-Alemán, Néstor J., Castañeda, Edgar, Berghe, Eric Van Den, Elías, Diego J., Mcmahan, Caleb D. & Matamoros, Wilfredo A., 2023, Continental fishes of Nicaragua: diversity, distribution and conservation status; with an annotated and illustrated checklist of species and an identification guide to families, pp. 1-89 in Zootaxa 5376 (1) on page 35, DOI: 10.11646/zootaxa.5376.1.1, http://zenodo.org/record/10208788
title: Coordination between deformation, precipitation, and erosion during orogenic growth
Creators: name: Yuan, Xiaoping
name: Li, Yuqiang
name: Brune, Sascha
name: Li, Kai
name: Pons, Michaël
name: Wolf, Sebastian G.
Publisher: name: Freie Universität Berlin
Year: 2024
Subjects: subject: Climate and Earth system modelling
subject: Geomorphology
subject: Tectonics
classificationCode: 550
subject: Geowissenschaften

description: Crustal thickening associated with orogenic growth elevates topography, causing orographic enhancement of precipitation, which in turn facilitates local erosion and possibly intensifies localization of deformation. How these three processes—deformation, precipitation, and erosion—coordinate during orogenic growth remains unknown. Here, we present a numerical model where tectonics, surface processes, and orographic precipitation are tightly coupled, and explore the impact on low, intermediate, and high erodibility orogens. We show that, for intermediate erodibility models, rock uplift rates and precipitation rates correlate well with erosion rates during the formation of orogenic plateaus with high correlation coefficients of ~0.9 between rock uplift and erosion rates, and ~0.8 between precipitation and erosion rates. We demonstrate a cyclicity of correlation evolution among uplift, precipitation, and erosion rates through the development of new faults propagating outward. These results shed insights into the relative tectonic or climatic control on erosion in active orogens (e.g., Himalayas, Central Andes, and Southern Alps of New Zealand), and provide a plausible explanation for several conflicting data and interpretations in the Himalayas, which depend on the stage of maturity of the newest fault and the relative locations to old faults.
Report of the Union County Mosquito Extermination Commission for 1941
Creators: name: Collins, Roi C.
Publisher: name: No Publisher Supplied
Year: 1941

description: A yearly series of reports from the Union County Mosquito Extermination Commission. The reports cover finances, inspections, drainage, oiling, and salt marsh, inland, and educational work regarding the extermination and control of mosquitoes in the different towns and districts of Union County, New Jersey.
Eodrilus Michaelsen 1907
Creators: name: Csuzdi, Csaba
name: Szederjesi, Tímea
name: Sherlock, Emma
Publisher: name: Zenodo
Year: 2018
Subjects: Biodiversity
Taxonomy
Animalia
Annelida
Clitellata
Opisthopora
Acanthodrilidae
Eodrilus

description: Genus Eodrilus Michaelsen, 1907
Remarks. The status of the South American Eodrilus species is quite controversial, as its type species Eo. cornigravei Michaelsen, 1907 toghether with the other Australian Eodrilus species was transferred to Diplotrema Spencer, 1900 (Jamieson & Dyne 1976; James 1990; Reynolds & Righi 1994; Blakemore 1997; Fragoso & Brown 2007). The South African Eodrilus species were removed and accomodated in Eodriloides Zicsi, 1998. The South American species are maintained here in Eodrilus, until a statisfactory solution is found, following Reynolds and Righi (1997) and Fragoso and Brown (2007).
description: Published as part of Csuzdi, Csaba, Szederjesi, Tímea & Sherlock, Emma, 2018, Annotated checklist of earthworm species described by András Zicsi (Clitellata: Megadrili), pp. 11-42 in Zootaxa 4496 (1) on page 13, DOI: 10.11646/zootaxa.4496.1.4, http://zenodo.org/record/1447011
Dendritic cell targeted vaccination
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Hematopoietic cells constantly turn over. Their rates of division and death determine the steady-state number of cells and size of lymphoid organs. In particular, T lymphocytes are held in a state of active quiescence that can be relieved to allow for dramatic expansions and contractions associated with infection or tumorigenesis. Whether there is a connection between active quiescence, homeostasis, regulation of antigen-mediated expansion is presently unknown. The hypothesis to be tested in this proposal is that some or all of these aspects of T cell and dendritic cell physiology are importantly regulated by a family of transcription factors known to integrate multiple inputs: growth factors, nutrients, and stress. This family has been termed FOXO for Forkhead bOX with the second O denoting one of 19 subfamilies consisting of four members related by sequence. We find that Foxo1 and Foxo3 are widely expressed, although they are not redundant. Deletion of individual Foxo genes has dramatic effects that can be mapped separately to T cells or dendritic cells. The experiments described in this application are designed to understand the mechanisms underlying the regulation of dendritic cell function by Foxo3, and determine how Foxo3 integrates external input to control lymphoproliferation. We have found that a germline deletion of Foxo3 (made by insertional mutation) has important effects on the progression of an immune response, but contrary to published reports, the effects are not T cell intrinsic. In response to a viral infection T cells expand to almost 3-fold greater numbers, yet the phenotype does not transfer with T cells. We now have evidence that Foxo3-deficient dendritic cells are super-efficient antigen-presenting cells and this enhancement depends upon IL-6. Our hypothesis is that Foxo3 is expressed in dendritic cells and serves to promote a suppressive pathway involving the tryptophan catabolic enzyme indoleamine 2,3-dioxygenase (IDO). Loss of this pathway allows dendritic cells to promote the superinduction of T cells. There is strong evidence that many cancers use the IDO pathway to create a suppressive environment for effector T cells and also give rise to the enhancement of regulatory T cells. We propose to use genetic manipulation of Foxo3 to understand the basis IDO-mediated immune suppression.
Report of the Union County Mosquito Extermination Commission for 1942
Creators: name: Collins, Roi C.
Publisher: name: No Publisher Supplied
Year: 1942

description: A yearly series of reports from the Union County Mosquito Extermination Commission. The reports cover finances, inspections, drainage, oiling, and salt marsh, inland, and educational work regarding the extermination and control of mosquitoes in the different towns and districts of Union County, New Jersey.
Report of the Union County Mosquito Extermination Commission, 1953
Creators: name: Martin, William H.
name: Dengler, Henry P.
name: Benninger, Albert J.
name: LaCorte, Nicholas S.
name: Tracy, W. R.
Publisher: name: No Publisher Supplied
Year: 1953

description: A yearly series of reports from the Union County Mosquito Extermination Commission. The reports cover finances, inspections, drainage, oiling, and salt marsh, inland, and educational work regarding the extermination and control of mosquitoes in the different towns and districts of Union County, New Jersey.
Eodrilus andrassyi Zicsi 1993
Creators: name: Csuzdi, Csaba
name: Szederjesi, Tímea
name: Sherlock, Emma
Publisher: name: Zenodo
Year: 2018
Subjects: Biodiversity
Taxonomy
Animalia
Annelida
Clitellata
Opisthopora
Acanthodrilidae
Eodrilus
Eodrilus andrassyi

description: Eodrilus andrassyi Zicsi, 1993
Eodrilus andrassyi Zicsi, 1993c Rev. suisse Zool. 100 (3): 631.
description: Published as part of Csuzdi, Csaba, Szederjesi, Tímea & Sherlock, Emma, 2018, Annotated checklist of earthworm species described by András Zicsi (Clitellata: Megadrili), pp. 11-42 in Zootaxa 4496 (1) on page 13, DOI: 10.11646/zootaxa.4496.1.4, http://zenodo.org/record/1447011
Role of AV Aids in Enhancing Students Academic Achievement at Secondary Level in Azad Jammu and Kashmir
Creators: name: Sunaina, Ghazal
Publisher: name: Zenodo
Year: 2024

description: This study was conducted to examine the role of AV aids in enhancing academic achievement at secondary level in City Kotli AJ&K. The current study was descriptive in nature and survey method was used for the data collection. The population of the study consisted of 304 secondary school teachers and 170 teachers selected using simple random sampling technique. The researcher personally visited the secondary schools of City Kotli and collected the data.  The researcher applied frequency, percentage, simple mean and one-way ANOVA for the analysis of data. It was found that there were significant differences between the role of AV aids and student’s academic achievement with respect to teacher’s qualifications and experiences. It is concluded that SSTs use educational videos to interact with digital or visual content. It is recommended that SSTs may use projectors, whiteboards, educational videos and multimedia presentations to present the visual content properly in the classroom.
Acridinae MacLeay 1821
Creators: name: Sultana, Riffat
name: Song, Hojun
Publisher: name: Zenodo
Year: 2024
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Orthoptera
Acrididae

description: Acridinae MacLeay, 1821
Tribe: Acridini MacLeay, 1821
Genus Acrida Linnaeus, 1758
1. Acrida exaltata (Walker, 1859)
2. Acrida gigantea (Herbst, 1786)
3. Acrida indica Dirsh, 1954
4. Acrida turrita (Linnaeus,1758)
Tribe: Phlaeobini Brunner von Wattenwyl, 1893
Genus Phlaeoba Stål, 1861
5. Phlaeoba infumata Brunner von Wattenwyl, 1893 6. Phlaeoba panteli Bolívar, 1902
7. Phlaeoba rotundata Uvarov, 1929
8. Phlaeoba tenebrosa (Walker, 1871)
9. Phlaeoba brachyptera Caudell, 1921
Genus Duroniella Bolívar, 1908
10. Duroniella laeviceps Uvarov, 1938
11. Duroniella laticornis (Krauss, 1909)
Tribe: Truxalini Serville, 1838
Genus Truxalis Fabricius, 1775
12. Truxalis eximia Eichwald, 1830
13. Truxalis fitzgeraldi Dirsh, 1950
14. Truxalis nasuta (Linnaeus, 1758)
15. Truxalis procera Klug, 1830
description: Published as part of Sultana, Riffat & Song, Hojun, 2024, Annotated catalogue of Pakistani Acrididae (Orthoptera: Caelifera: Acridoidea), pp. 1-47 in Zootaxa 5486 (1) on page 5, DOI: 10.11646/zootaxa.5486.1.1, http://zenodo.org/record/13209743