title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ Ð³Ð»ÑÑÐ°ÑÐ¸Ð¾Ð½-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾Ð¹ ÑÐµÑÐ¼ÐµÐ½ÑÐ°ÑÐ¸Ð²Ð½Ð¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ñ Ð½Ð° ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð°Ð½ÑÐ¸Ð¾ÐºÑÐ¸Ð´Ð°Ð½ÑÐ½Ð¾Ð¹ Ð·Ð°ÑÐ¸ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð-562
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Effect of the glutathione-dependent enzymatic system on the effectiveness of antioxidant protection of K-562 cells
Creators: name: ÐÐ°Ð·Ð¸Ð·Ð¾Ð²Ð°, ÐÐ»ÑÐ·Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸ ÐºÐ¾Ð´Ð¸ÑÑÐµÐ¼ÑÐ¹ ÑÐµÐ½ÑÐ¾Ñ Ð¿ÐµÑÐµÐºÐ¸ÑÐ¸ Ð²Ð¾Ð´Ð¾ÑÐ¾Ð´Ð° HyPer
H2O2
Ð³Ð»ÑÑÐ°ÑÐ¸Ð¾Ð½ (GSH)
ÐºÐ»ÐµÑÐºÐ¸ Ð-562
Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½Ð°Ñ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ñ Ð¿ÐµÑÐµÐºÐ¸ÑÐ¸ Ð²Ð¾Ð´Ð¾ÑÐ¾Ð´Ð°
Ð¾ÐºÐ¸ÑÐ»Ð¸ÑÐµÐ»ÑÐ½ÑÐ¹ ÑÑÑÐµÑÑ
Ð°ÐºÑÐ¸Ð²Ð½ÑÐµ ÑÐ¾ÑÐ¼Ñ ÐºÐ¸ÑÐ»Ð¾ÑÐ¾Ð´Ð°
Ð±ÑÑÐ¸Ð¾Ð½Ð¸Ð½ ÑÑÐ»ÑÑÐ¾ÐºÑÐ¸Ð¼Ð¸Ð½
Cout/Cin
ratio
genetically encoded sensor of hydrogen peroxide HyPer
glutathione (GSH)
K-562 cells
intracellular concentration of hydrogen peroxide
oxidative stress
reactive oxygen species
butyonine sulfoximine

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¾ÑÐµÐ½ÐºÐµ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð³Ð»ÑÑÐ°ÑÐ¸Ð¾Ð½-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾Ð¹ ÑÐµÑÐ¼ÐµÐ½ÑÐ°ÑÐ¸Ð²Ð½Ð¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ñ Ð½Ð° Ð°Ð½ÑÐ¸Ð¾ÐºÑÐ¸Ð´Ð°Ð½ÑÐ½ÑÐ¹ ÑÑÑÐµÐºÑ ÐºÐ»ÐµÑÐ¾Ðº Ð-562 Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸ ÐºÐ¾Ð´Ð¸ÑÑÐµÐ¼Ð¾Ð³Ð¾ ÑÐµÐ½ÑÐ¾ÑÐ° Ð¿ÐµÑÐµÐºÐ¸ÑÐ¸ Ð²Ð¾Ð´Ð¾ÑÐ¾Ð´Ð° HyPer. Ð¦ÐµÐ»Ñ ÑÐ°Ð±Ð¾ÑÑ: Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÑ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð³Ð»ÑÑÐ°ÑÐ¸Ð¾Ð½-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾Ð¹ ÑÐµÑÐ¼ÐµÐ½ÑÐ°ÑÐ¸Ð²Ð½Ð¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ñ Ð½Ð° ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð°Ð½ÑÐ¸Ð¾ÐºÑÐ¸Ð´Ð°Ð½ÑÐ½Ð¾Ð¹ Ð·Ð°ÑÐ¸ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð-562 Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ Ð²Ð½ÐµÑÐ½ÐµÐ³Ð¾ Ð¾ÐºÐ¸ÑÐ»Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ ÑÑÑÐµÑÑÐ° ÑÐ°Ð·Ð½Ð¾Ð¹ Ð¸Ð½ÑÐµÐ½ÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸. Ð Ð°Ð±Ð¾ÑÐ° Ð±ÑÐ»Ð° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð² ÐÐ°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐ¸Ð³Ð½Ð°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð¤ÐÐ£ÐÐ ÐÐ½ÑÑÐ¸ÑÑÑ ÑÐ¸ÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð ÐÐ. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð¿ÑÐ¾Ð´ÐµÐ»Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¸ Ð¾ÑÑÐ°Ð±Ð¾ÑÐ°Ð½Ñ Ð¼ÐµÑÐ¾Ð´Ñ, Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÑÑÐ¸Ðµ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²ÐµÐ½Ð½Ð¾ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÑÑÑ Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ñ Ð¿ÐµÑÐµÐºÐ¸ÑÐ¸ Ð²Ð¾Ð´Ð¾ÑÐ¾Ð´Ð° Ð² ÐºÐ»ÐµÑÐºÐ°Ñ Ð-562 Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ Ð²Ð½ÐµÑÐ½ÐµÐ³Ð¾ H2O2-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ ÑÑÑÐµÑÑÐ°, Ð±ÑÐ»Ð° Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð° ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ñ H2O2, ÑÑÑÐ°Ð½Ð°Ð²Ð»Ð¸Ð²Ð°ÑÑÐ°ÑÑÑ Ð² ÐºÐ»ÐµÑÐºÐ°Ñ Ð-562 Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ Ð²Ð½ÐµÑÐ½ÐµÐ³Ð¾ ÑÐ¸ÑÐ¾ÑÐ¾ÐºÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¸ Ð½ÐµÑÐ¸ÑÐ¾ÑÐ¾ÐºÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ H2O2-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ ÑÑÑÐµÑÑÐ°, Ð±ÑÐ»Ð° Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð° ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ñ H2O2, ÑÑÑÐ°Ð½Ð°Ð²Ð»Ð¸Ð²Ð°ÑÑÐ°ÑÑÑ Ð² ÐºÐ»ÐµÑÐºÐ°Ñ Ð-562 Ð¿ÑÐ¸ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ ÑÐ¸ÑÑÐµÐ¼Ñ Ð³Ð»ÑÑÐ°ÑÐ¸Ð¾Ð½Ð° Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ Ð²Ð½ÐµÑÐ½ÐµÐ³Ð¾ H2O2-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ ÑÑÑÐµÑÑÐ°.
description: This work is devoted to assessing the effect of the glutathione-dependent enzymatic system on the antioxidant effect of K-562 cells using the HyPer genetically encoded hydrogen peroxide sensor. Objective: to investigate the effect of the glutathione-dependent enzymatic system on the effectiveness of the antioxidant defense of K-562 cells under conditions of external oxidative stress of varying intensity. The work was carried out at the Laboratory of Intracellular Signaling, Institute of Cytology, Russian Academy of Sciences. As a result of the work done, methods were developed to quantify the intracellular concentration of hydrogen peroxide in K-562 cells under conditions of external H2O2-induced stress, the concentration of H2O2 established in K-562 cells under conditions of external cytotoxic and non-cytotoxic H2O2-induced stress was determined, the concentration of H2O2 established in K-562 cells upon inhibition of the glutathione system under conditions of external H2O2-induced stress was determined.