title: Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð¼ÐµÑÐ¾Ð´Ð° Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ Ð¼ÑÑÐ°ÑÐ¸Ð¹ Ð² ÑÐ°Ð¿ÐµÑÐ¾Ð½Ðµ SIS1, Ð²Ð»Ð¸ÑÑÑÐ¸Ñ Ð½Ð° ÑÐ°ÑÐ¿ÑÐ¾ÑÑÑÐ°Ð½ÐµÐ½Ð¸Ðµ Ð¿ÑÐ¸Ð¾Ð½Ð¾Ð² Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Development of a method for receiving mutations in the SIS1 chaperone that affect the propagation of yeast prions
Creators: name: ÐÑÐ»Ð¸ÐºÐ¾Ð²Ð°, ÐÐ»ÐµÐºÑÐ°Ð½Ð´ÑÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: Ð¿ÑÐ¸Ð¾Ð½Ñ Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹
Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½ÑÐµ ÑÐ°Ð¿ÐµÑÐ¾Ð½Ñ
SIS1
HSP104
Ð°Ð³ÑÐµÐ³Ð°ÑÑ
HSP40
HSP70
ÑÑÐ°Ð¼Ð¼Ñ Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹
ÑÐ²ÐµÑÑ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ
yeast prions
molecular chaperones
aggregates
yeast strains
overexpression

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ð¿ÑÐ¸Ð¾Ð½Ð¾Ð² Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹ Ñ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½ÑÐ¼Ð¸ ÑÐ°Ð¿ÐµÑÐ¾Ð½Ð°Ð¼Ð¸. Ð¦ÐµÐ»Ñ Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ ââ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð¼ÐµÑÐ¾Ð´Ð° ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾Ð¹ Ð¸Ð´ÐµÐ½ÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Ð¼ÑÑÐ°ÑÐ¸Ð¹ Ð² ÑÐ°Ð¿ÐµÑÐ¾Ð½Ðµ SIS1, Ð²Ð»Ð¸ÑÑÑÐ¸Ñ Ð½Ð° ÑÐ°ÑÐ¿ÑÐ¾ÑÑÑÐ°Ð½ÐµÐ½Ð¸Ðµ Ð¿ÑÐ¸Ð¾Ð½Ð¾Ð² Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð¸Ñ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¸ ÑÐµÑÐµÐ½Ñ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð·Ð°Ð´Ð°ÑÐ¸: 1. ÐÐ¾Ð»ÑÑÐ¸ÑÑ Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼ÑÐµ ÑÑÐ°Ð¼Ð¼Ñ Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹ Ð¸ Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð´Ð½ÑÐµ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ð¸. 2. ÐÑÐ¾Ð°Ð½Ð°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°ÑÑ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ ÑÐ²ÐµÑÑÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ HSP104 Ð½Ð° Ð¿Ð¾Ð´Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ðµ Ð¿ÑÐ¸Ð¾Ð½Ð° [PSI+] Ð² Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ ÑÑÐ°Ð¼Ð¼Ð°Ñ. 3. Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐ°ÑÑ Ð¸ Ð¿ÑÐ¾Ð²ÐµÑÐ¸ÑÑ Ð¼ÐµÑÐ¾Ð´ ÑÐ¾ÑÐµÐ²Ð½Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½ÑÑ ÐºÑÐ»ÑÑÑÑ Ð´Ð»Ñ Ð¸Ð´ÐµÐ½ÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Ð¼ÑÑÐ°ÑÐ¸Ð¹, Ð²Ð»Ð¸ÑÑÑÐ¸Ñ Ð½Ð° ÑÐ°ÑÐ¿ÑÐ¾ÑÑÑÐ°Ð½ÐµÐ½Ð¸Ðµ Ð¿ÑÐ¸Ð¾Ð½Ð°. Ð Ð°Ð±Ð¾ÑÐ° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð³ÐµÐ½ÐµÑÐ¸ÐºÐ¸ ÐºÐ°ÑÐµÐ´ÑÑ Ð³ÐµÐ½ÐµÑÐ¸ÐºÐ¸ Ð¸ Ð±Ð¸Ð¾ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¡ÐÐ±ÐÐ£. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð±ÑÐ»Ð° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° ÑÐµÑÐ¸Ñ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ¾Ð² Ð¿Ð¾ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ Ð½Ð¾Ð²ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð² Ð¸ Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð´Ð½ÑÑ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ð¹. ÐÐ½Ð°Ð»Ð¸Ð· Ð²Ð»Ð¸ÑÐ½Ð¸Ñ ÑÐ²ÐµÑÑÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ HSP104 Ð½Ð° Ð¿Ð¾Ð´Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ðµ Ð¿ÑÐ¸Ð¾Ð½Ð° [PSI+] Ð¿Ð¾Ð´ÑÐ²ÐµÑÐ´Ð¸Ð» Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐ°Ð½ÐµÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ. Ð ÑÐ¾Ð´Ðµ Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ Ð½Ð° Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ ÑÑÐ°Ð¼Ð¼Ð°Ñ Ð¸ Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð´Ð°Ñ Ð¾ÑÑÐ°Ð±Ð¾ÑÐ°Ð½ Ð¼ÐµÑÐ¾Ð´ ÑÐ¾ÑÐµÐ²Ð½Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½ÑÑ ÐºÑÐ»ÑÑÑÑ.
description: The given work is devoted to the study of the interaction of yeast prions with molecular chaperones. The aim of the final work is to develop a method for the effective identification of mutations in the SIS1 chaperone that affect the propagation of yeast prions. As a result of the work, the following tasks were solved: 1. Receiving the necessary yeast strains and plasmid constructs. 2. Analysis the effect of overexpression of HSP104 on the maintenance of the prion [PSI+] in the obtained strains. 3. Development and test a competitive culture method for identifying mutations that affect prion propagation. The work was carried out on the basis of the Laboratory of Physiological Genetics of the Department of Genetics and Biotechnology of St. Petersburg State University. As a result, a series of experiments were conducted to obtain new strains and plasmid constructs. Analysis of the effect of HSP104 overexpression on prion maintenance [PSI+] confirmed the previously obtained results. In the course of the final qualification work, the method of competitive cultures was developed on the obtained strains and plasmids.