title: Ð¡ÑÐ°Ð²Ð½Ð¸ÑÐµÐ»ÑÐ½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð· Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ Ñ ÑÐ¾Ð¼Ð°ÑÐ¸Ð½-ÑÐµÐ¼Ð¾Ð´ÑÐ»Ð¸ÑÑÑÑÐ¸Ñ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð² Ð² Ð¿Ð¾Ð»Ð¸ÑÐµÐ½Ð½ÑÑ Ñ ÑÐ¾Ð¼Ð¾ÑÐ¾Ð¼Ð°Ñ Drosophila melanogaster
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Comparative analysis of the localization of chromatin-remodeling factors in polytene chromosomes of Drosophila melanogaster
Creators: name: ÐÐµÐºÑÐ°ÑÐ¾Ð²Ð°, ÐÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: CHD1
ISWI
Kismet
Brahma
Drosophila melanogaster
Ð¿Ð¾Ð»Ð¸ÑÐµÐ½Ð½ÑÐµ Ñ ÑÐ¾Ð¼Ð¾ÑÐ¾Ð¼Ñ
Ñ ÑÐ¾Ð¼Ð°ÑÐ¸Ð½
Ð´Ð¾Ð·Ð¾Ð²Ð°Ñ ÐºÐ¾Ð¼Ð¿ÐµÐ½ÑÐ°ÑÐ¸Ñ
polytene chromosome
chromatin
dosage compensation

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«Ð¡ÑÐ°Ð²Ð½Ð¸ÑÐµÐ»ÑÐ½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð· Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ ÑÑÐ¾Ð¼Ð°ÑÐ¸Ð½-ÑÐµÐ¼Ð¾Ð´ÑÐ»Ð¸ÑÑÑÑÐ¸Ñ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð² Ð² Ð¿Ð¾Ð»Ð¸ÑÐµÐ½Ð½ÑÑ ÑÑÐ¾Ð¼Ð¾ÑÐ¾Ð¼Ð°Ñ Drosophila melanogasterÂ». ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° ÑÑÐ°Ð²Ð½Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð¼Ñ Ð°Ð½Ð°Ð»Ð¸Ð·Ñ Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ ÑÑÐ¾Ð¼Ð°ÑÐ¸Ð½-ÑÐµÐ¼Ð¾Ð´ÑÐ»Ð¸ÑÑÑÑÐ¸Ñ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð² Ð² Ð¿Ð¾Ð»Ð¸ÑÐµÐ½Ð½ÑÑ ÑÑÐ¾Ð¼Ð¾ÑÐ¾Ð¼Ð°Ñ Drosophila melanogaster. ÐÐ· Ð²ÑÐµÐ³Ð¾ ÑÑÐ´Ð° ÑÑÐ¾Ð¼Ð°ÑÐ¸Ð½-ÑÐµÐ¼Ð¾Ð´ÑÐ»Ð¸ÑÑÑÑÐ¸Ñ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð² Ð±ÐµÐ»Ð¾Ðº CHD1 Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÑÐµÑ Ð¾ÑÐ¾Ð±ÑÐ¹ Ð¸Ð½ÑÐµÑÐµÑ, Ð±Ð»Ð°Ð³Ð¾Ð´Ð°ÑÑ ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐµÑÐºÐ¾Ð¼Ñ Ð¿ÑÐ¸Ð²Ð»ÐµÑÐµÐ½Ð¸Ñ Ðº X-ÑÑÐ¾Ð¼Ð¾ÑÐ¾Ð¼Ðµ Ñ ÑÐ°Ð¼ÑÐ¾Ð² Drosophila melanogaster. ÐÐ¾Ð¼Ð¸Ð¼Ð¾ CHD1 Ð¸Ð·Ð²ÐµÑÑÐ½Ñ Ð´ÑÑÐ³Ð¸Ðµ ÑÐ°ÐºÑÐ¾ÑÑ ÑÐµÐ¼Ð¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÑÑÐ¾Ð¼Ð°ÑÐ¸Ð½Ð°, ÑÐ°ÐºÐ¸Ðµ ÐºÐ°Ðº ISWI, Brahma Ð¸ Kismet. ÐÐµÑÐ¾Ð´Ð¾Ð¼ Ð¸Ð¼Ð¼ÑÐ½Ð¾Ð¾ÐºÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ñ Ð¸ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸ Ð±ÑÐ»Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ñ Ð¸Ð·Ð¾Ð±ÑÐ°Ð¶ÐµÐ½Ð¸Ñ Ñ Ð½Ð°Ð³Ð»ÑÐ´Ð½Ð¾Ð¹ Ð´ÐµÐ¼Ð¾Ð½ÑÑÑÐ°ÑÐ¸ÐµÐ¹ (ÑÐ²ÐµÑÐ¾Ð¼) ÑÐ°ÑÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ ÑÑÐ¾Ð¼Ð°ÑÐ¸Ð½-ÑÐµÐ¼Ð¾Ð´ÑÐ»Ð¸ÑÑÑÑÐ¸Ñ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð² Ð½Ð° Ð¿Ð¾Ð»Ð¸ÑÐµÐ½Ð½ÑÑ ÑÑÐ¾Ð¼Ð¾ÑÐ¾Ð¼Ð°Ñ. ÐÐ¾ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ°Ð¼ ÑÑÐ°Ð²Ð½Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð´Ð°Ð½Ð½ÑÑ Ð¸Ð·Ð¾Ð±ÑÐ°Ð¶ÐµÐ½Ð¸Ð¹ ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¿ÑÐ¸Ð²Ð»ÐµÑÐµÐ½Ð¸Ñ Ðº Ð¥-ÑÑÐ¾Ð¼Ð¾ÑÐ¾Ð¼Ðµ ÑÐ°Ð¼ÑÐ¾Ð² Ð´Ð»Ñ Ð±ÐµÐ»ÐºÐ¾Ð² ISWI, Brahma Ð¸ Kismet Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¾ Ð½Ðµ Ð±ÑÐ»Ð¾. ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð±ÐµÐ»ÐºÐ¾Ð² ISWI, Brahma Ð¸ Kismet Ñ Ð¼ÑÑÐ°Ð½ÑÐ½ÑÑ Ð¿Ð¾ Ð³ÐµÐ½Ñ Chd1 ÑÐ°Ð¼ÑÐ¾Ð² ÑÐ°ÐºÐ¶Ðµ Ð½Ðµ Ð²ÑÑÐ²Ð¸Ð»Ð¾ ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¿ÑÐ¸Ð²Ð»ÐµÑÐµÐ½Ð¸Ñ Ðº Ð¥-ÑÑÐ¾Ð¼Ð¾ÑÐ¾Ð¼Ðµ Ð¸ Ð¿ÐµÑÐµÑÐ°ÑÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ ÑÑÐ¸Ñ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð². Ð¢Ð°ÐºÐ¸Ð¼ Ð¾Ð±ÑÐ°Ð·Ð¾Ð¼, CHD1 ÑÐ²Ð»ÑÐµÑÑÑ ÑÐ½Ð¸ÐºÐ°Ð»ÑÐ½ÑÐ¼ ÑÑÐµÐ´Ð¸ ÑÑÐ¾Ð¼Ð°ÑÐ¸Ð½-ÑÐµÐ¼Ð¾Ð´ÐµÐ»Ð¸ÑÑÑÑÐ¸Ñ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð² Ð² Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ð¸ Ð¿ÑÐ¸Ð²Ð»ÐµÑÐµÐ½Ð¸Ñ Ðº Ð¥-ÑÑÐ¾Ð¼Ð¾ÑÐ¾Ð¼Ðµ Ñ ÑÐ°Ð¼ÑÐ¾Ð² Ð¸ Ð½Ðµ Ð¼Ð¾Ð¶ÐµÑ Ð±ÑÑÑ Ð·Ð°Ð¼ÐµÐ½ÐµÐ½ Ð´ÑÑÐ³Ð¸Ð¼Ð¸ ÑÐ°ÐºÑÐ¾ÑÐ°Ð¼Ð¸. ÐÐ°Ð½Ð½Ð¾Ðµ Ð·Ð°ÐºÐ»ÑÑÐµÐ½Ð¸Ðµ Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÐµÑ Ð¿ÑÐµÐ´Ð¿Ð¾Ð»Ð¾Ð¶Ð¸ÑÑ Ð½Ð°Ð»Ð¸ÑÐ¸Ðµ Ñ CHD1 ÑÑÐ´Ð° ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÑÐ½ÐºÑÐ¸Ð¹, Ð²Ð¾Ð²Ð»ÐµÑÐµÐ½Ð½ÑÑ Ð² Ð¿ÑÐ¾ÑÐµÑÑ Ð´Ð¾Ð·Ð¾Ð²Ð¾Ð¹ ÐºÐ¾Ð¼Ð¿ÐµÐ½ÑÐ°ÑÐ¸Ð¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÐµÑÑ Ð¿ÑÐµÐ´ÑÑÐ¾Ð¸Ñ Ð¸Ð·ÑÑÐ¸ÑÑ.
description: The subject of the graduate qualification work is the âComparative analysis of the localization of chromatin-remodeling factors in polytene chromosomes of Drosophila melanogasterâ. This work is devoted to a comparative analysis of the localization of chromatin-remodeling factors in polytene chromosomes of Drosophila melanogaster. Among the of chromatin-remodeling factors, the CHD1 protein is of particular interest due to its specific recruitment to the X-chromosome in Drosophila melanogaster males. Besides CHD1, we analyzed the distribution in polytene chromosomes of such chromatin remodeling factors as ISWI, Brahma, and Kismet. Using immunostaining and fluorescence microscopy, we obtained the good resolution images (in color) of the chromatin-remodulating factors distribution in polytene chromosomes. Comparative analysis of the imaging data revealed no specific enrichment of the male X-chromosome for ISWI, Brahma, and Kismet proteins. Thus, CHD1 is unique among chromatin remodeling factors in terms of specific recruitment to the male X-chromosome. This finding suggests that CHD1 has some specific functions in the dosage compensation process that have yet to be explored.