Additional file 1 of Self-assembly of H2S-responsive nanoprodrugs based on natural rhein and geraniol for targeted therapy against Salmonella Typhimurium
Creators: name: Han, Lu
name: Zang, Tao
name: Tan, Lulu
name: Liang, Dunsheng
name: Long, Tengfei
name: Liu, Xuwei
name: Shen, Xiaofan
name: Ren, Hao
name: Li, ZhiPeng
name: Lu, Zhaoxiang
name: Tang, Shengqiu
name: Liao, Xiaoping
name: Liu, Yahong
name: Zhang, Chaoqun
name: Sun, Jian
Publisher: name: figshare
Year: 2024
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Pharmacology
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Immunology
subject: FOS: Clinical medicine
subject: Mental Health
subject: Infectious Diseases
subject: FOS: Health sciences

description: Additional file 1: Table S1. PCR primers for inflammatory factor genes and bacterial identification. Table S2. Gel permeation chromatography results for synthetic compounds used in this study. Figure S1. Synergism of rhein and geraniol in vitro. (A) Checkerboard assay of rhein and geraniol. (B) Time-killing curves of rhein and geraniol. Figure S2. 1H NMR and FTIR characteristics of PPG and PPR. Structural formulas of (A) PPR and (B) PPG. 1H NMR spectra of (C) PPR and (D) PPG. FTIR spectra of (E) PPR and (F) PPG. Figure S3. XPS spectra of the PPR and PPRG. Figure S4. The XRD spectra of poly(α-lipoic acid), PPR, PRMG, and rhein. Figure S5. Rhein and geraniol release amount from PPRG nanoprodrug in dissolve medium. (A) Rhein release amount from PPR and PPRG nanoprodrugs at pH 7.4 containing 1 mM DTT, (B) Geraniol release amount from PPG and PPRG nanoprodrugs at pH 7.4 containing 1 mM DTT. (C) 14-day room temperature storage stability test of PPRG. Figure S6. Cell viability of (A) HGF-1 and (B) Hep G2. (C) Live/dead cell staining of 293 T cells. Test samples were added to cells at 40 µM each. CON, control; SAL, Salmonella; RH, rhein; PPR, PEGylated poly (α-lipoic acid)-grafted rhein; GE, geraniol; PPG, PEGylated poly (α-lipoic acid)-grafted geraniol; PPRG, Poly(α-lipoic acid)-polyethylene glycol grafted rhein and geraniol. Figure S7. Apparent drug permeability in Caco-2 cells. (A) Transepithelial electrical resistance (TEER) of Caco-2 cells. (B) The permeability of the rhein, PPR and PPRG compounds. (C) The permeability of the geraniol, PPG and PPRG compounds. Figure S8. Intestinal morphology of mice in the indicated test groups. H&amp;E staining of duodenum, ileum, and conlon. Figure S9. The performance of the mice in the Morris water maze test. (A) The timeline of drug treatment, spatial acquisition and probe trial sessions of the Morris water maze. (B) The body weight changes during the whole experiment. (C) Representative swimming paths of the mice receiving PBS, rhein, geraniol or PPRG treatment during the probe trial. (D-E) The path length and escape latency in the four groups over six consecutive training days. (F) Platform crossing (G) Passing Times (H) Escape latency on the 7th day of the spatial acquisition session. Figure S10. Average plasma concentration-time curve of (A) rhein, (B) PPRG-rhein, (C) geraniol, and (D) PPRG-geraniol after intragastric administration in rats (n=4). Table S3. Pharmacokinetic parameters of drugs in rats after oral administration. Table S4. Abbreviations.