title: ÐÐ·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¿Ð»Ð°ÑÑÐ¸ÑÐ½Ð¾ÑÑÐ¸ Ð² Ñ ÑÐ¾Ð½Ð¸ÑÐµÑÐºÑÑ ÑÐ°Ð·Ñ Ð»Ð¸ÑÐ¸Ð¹-Ð¿Ð¸Ð»Ð¾ÐºÐ°ÑÐ¿Ð¸Ð½Ð¾Ð²Ð¾Ð¹ Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð²Ð¸ÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Changes in synaptic plasticity in the chronic phase of the lithium-pilocarpine model of temporal epilepsy
Creators: name: ÐÐ¸ÐµÑÐ¿Ð¸ÑÐ¾Ð², ÐÐµÐ¾ÑÐ³Ð¸Ð¹
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÐÐ¸Ð¾ÑÐµÑ Ð½Ð¾Ð»Ð¾Ð³Ð¸Ñ
Ð²Ð¸ÑÐ¾ÑÐ½Ð°Ñ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ñ
Ð»Ð¸ÑÐ¸Ð¹-Ð¿Ð¸Ð»Ð¾ÐºÐ°ÑÐ¿Ð¸Ð½Ð¾Ð²Ð°Ñ Ð¼Ð¾Ð´ÐµÐ»Ñ
ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ°Ñ Ð¿Ð»Ð°ÑÑÐ¸ÑÐ½Ð¾ÑÑÑ
Ð´Ð¾Ð»Ð³Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð°Ñ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°ÑÐ¸Ñ
nmda-ÑÐµÑÐµÐ¿ÑÐ¾ÑÑ
Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿
temporal lobe epilepsy
lithium-pilocarpine model
synaptic plasticity
long-term potentiation
nmda receptors
hippocampus

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«ÐÐ·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¿Ð»Ð°ÑÑÐ¸ÑÐ½Ð¾ÑÑÐ¸ Ð² ÑÑÐ¾Ð½Ð¸ÑÐµÑÐºÑÑ ÑÐ°Ð·Ñ Ð»Ð¸ÑÐ¸Ð¹-Ð¿Ð¸Ð»Ð¾ÐºÐ°ÑÐ¿Ð¸Ð½Ð¾Ð²Ð¾Ð¹ Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð²Ð¸ÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ð¸Â». ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½ÑÑ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð¾Ð² ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð´Ð¾Ð»Ð³Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð¹ ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¿Ð»Ð°ÑÑÐ¸ÑÐ½Ð¾ÑÑÐ¸ Ð² Ð»Ð¸ÑÐ¸Ð¹-Ð¿Ð¸Ð»Ð¾ÐºÐ°ÑÐ¿Ð¸Ð½Ð¾Ð²Ð¾Ð¹ Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð²Ð¸ÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ð¸ (ÐÐ­). ÐÐ¾ÑÐ»ÐµÐ´Ð½Ð¸Ðµ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¿Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÑÑ, ÑÑÐ¾ Ð¿Ð¾ÑÐ»Ðµ Ð¿ÐµÑÐµÐ½ÐµÑÐµÐ½Ð½ÑÑ ÑÑÐ´Ð¾ÑÐ¾Ð¶Ð½ÑÑ ÑÐ¾ÑÑÐ¾ÑÐ½Ð¸Ð¹ ÐµÑ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ¸ Ð² Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ðµ Ð¿Ð¾Ð´Ð²ÐµÑÐ³Ð°ÑÑÑÑ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸ÑÐ¼. ÐÐ´Ð½Ð°ÐºÐ¾ ÑÐ°ÑÐ°ÐºÑÐµÑ ÑÑÐ¸Ñ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹ Ð¸ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ñ, Ð»ÐµÐ¶Ð°ÑÐ¸Ðµ Ð² Ð¸Ñ Ð¾ÑÐ½Ð¾Ð²Ðµ, Ð´Ð¾ ÑÐ¸Ñ Ð¿Ð¾Ñ Ð¾ÑÑÐ°ÑÑÑÑ Ð½ÐµÑÑÐ½ÑÐ¼Ð¸. Ð¦ÐµÐ»ÑÑ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¾ Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð¾Ð² ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð´Ð¾Ð»Ð³Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð¹ ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°ÑÐ¸Ð¸ Ð² Ð¿Ð¾Ð»Ðµ CA1 Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð° ÐºÑÑÑ Ð² ÑÑÐ¾Ð½Ð¸ÑÐµÑÐºÑÑ ÑÐ°Ð·Ñ Ð»Ð¸ÑÐ¸Ð¹-Ð¿Ð¸Ð»Ð¾ÐºÐ°ÑÐ¿Ð¸Ð½Ð¾Ð²Ð¾Ð¹ Ð¼Ð¾Ð´ÐµÐ»Ð¸ ÐÐ­.ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð¸Ñ ÑÐ°Ð±Ð¾ÑÑ: 1. ÐÑÐµÐ½Ð¸ÑÑ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ¸ Ð²Ð¾Ð·Ð±ÑÐ¶Ð´Ð°ÑÑÐµÐ¹ ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¿ÐµÑÐµÐ´Ð°ÑÐ¸ Ð² Ð¿Ð¾Ð»Ðµ CA1 Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð°. 2. ÐÐ·ÑÑÐ¸ÑÑ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð±Ð¸Ð¾ÑÐ¸Ð·Ð¸ÑÐµÑÐºÐ¸Ñ ÑÐ²Ð¾Ð¹ÑÑÐ² Ð¿Ð¸ÑÐ°Ð¼Ð¸Ð´Ð½ÑÑ Ð½ÐµÐ¹ÑÐ¾Ð½Ð¾Ð² Ð¿Ð¾Ð»Ñ CA1 Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð°. 3. ÐÑÐµÐ½Ð¸ÑÑ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° Ð´Ð¾Ð»Ð³Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð¹ ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°ÑÐ¸Ð¸ (ÐÐÐ) Ð² Ð¿Ð¾Ð»Ðµ CA1 Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð°. 4. ÐÑÐµÐ½Ð¸ÑÑ ÑÐ¾Ð»Ñ NMDA-ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð² Ð² ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ ÐÐÐ Ð² Ð¿Ð¾Ð»Ðµ CA1 Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð°. 5. ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÑ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ¸ ÑÐ¾ÐºÐ¾Ð², Ð¿ÑÐ¾ÑÐµÐºÐ°ÑÑÐ¸Ñ ÑÐµÑÐµÐ· NMDA-ÑÐµÑÐµÐ¿ÑÐ¾ÑÑ, Ð¾Ð¿Ð¾ÑÑÐµÐ´Ð¾Ð²Ð°Ð½Ð½ÑÐµ Ð²ÑÑÐ¾ÐºÐ¾ÑÐ°ÑÑÐ¾ÑÐ½Ð¾Ð¹ ÑÑÐ¸Ð¼ÑÐ»ÑÑÐ¸ÐµÐ¹, Ð½Ð° Ð¿Ð¸ÑÐ°Ð¼Ð¸Ð´Ð½ÑÑ Ð½ÐµÐ¹ÑÐ¾Ð½Ð°Ñ Ð² Ð¿Ð¾Ð»Ðµ CA1 Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð°. 6. ÐÐ·ÑÑÐ¸ÑÑ Ð²ÐºÐ»Ð°Ð´ Ð¼ÐµÑÐ°Ð±Ð¾ÑÑÐ¾Ð¿Ð½ÑÑ Ð³Ð»ÑÑÐ°Ð¼Ð°ÑÐ½ÑÑ ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð² I ÑÐ¸Ð¿Ð° mGluR1 Ð² Ð¸Ð½Ð´ÑÐºÑÐ¸Ñ ÐÐÐ Ð² Ð¿Ð¾Ð»Ðµ CA1 Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð°. Ð Ð°Ð±Ð¾ÑÐ° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ ÐÐ½ÑÑÐ¸ÑÑÑÐ° ÑÐ²Ð¾Ð»ÑÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¸ Ð±Ð¸Ð¾ÑÐ¸Ð¼Ð¸Ð¸ Ð¸Ð¼ÐµÐ½Ð¸ Ð. Ð. Ð¡ÐµÑÐµÐ½Ð¾Ð²Ð° Ð ÐÐ Ð² Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½ÑÑ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð¾Ð² Ð½ÐµÐ¹ÑÐ¾Ð½Ð½ÑÑ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ð¹. ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð¾ Ð½Ð° ÑÐ°Ð¼ÑÐ°Ñ ÐºÑÑÑ ÐÐ¸ÑÑÐ°Ñ ÑÐµÑÐµÐ· 30 Ð´Ð½ÐµÐ¹ Ð¿Ð¾ÑÐ»Ðµ Ð¿Ð¸Ð»Ð¾ÐºÐ°ÑÐ¿Ð¸Ð½-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÑÐ°ÑÑÑÐ° (Ð­Ð¡). ÐÐ»Ñ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ Ð¿ÑÐ¸Ð¼ÐµÐ½ÑÐ»Ð°ÑÑ Ð¼ÐµÑÐ¾Ð´Ð¸ÐºÐ° Ð¾ÑÐ²ÐµÐ´ÐµÐ½Ð¸Ñ Ð¿Ð¾Ð»ÐµÐ²ÑÑ Ð²Ð¾Ð·Ð±ÑÐ¶Ð´Ð°ÑÑÐ¸Ñ Ð¿Ð¾ÑÑÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð¾Ð² Ð¾Ñ ÑÐ°Ð´Ð¸Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÐ»Ð¾Ñ Ð¿Ð¾Ð»Ñ Ð¡Ð1 Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð°. ÐÐÐ Ð²ÑÐ·ÑÐ²Ð°Ð»Ð¸ Ð²ÑÑÐ¾ÐºÐ¾ÑÐ°ÑÑÐ¾ÑÐ½Ð¾Ð¹ ÑÑÐ¸Ð¼ÑÐ»ÑÑÐ¸ÐµÐ¹. Ð¡ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¼ÐµÑÐ¾Ð´Ð° Ð¿Ð°ÑÑ-ÐºÐ»Ð°Ð¼Ð¿ Ð² ÑÐµÐ¶Ð¸Ð¼Ðµ Â«ÑÐµÐ»Ð°Ñ ÐºÐ»ÐµÑÐºÐ°Â» Ð¸Ð·ÑÑÐ°Ð»Ð¸ÑÑ NMDA-Ð¾Ð¿Ð¾ÑÑÐµÐ´Ð¾Ð²Ð°Ð½Ð½ÑÐµ ÑÐ¾ÐºÐ¸ Ð² Ð¿Ð¸ÑÐ°Ð¼Ð¸Ð´Ð½ÑÑ Ð½ÐµÐ¹ÑÐ¾Ð½Ð°Ñ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð°, Ð° ÑÐ°ÐºÐ¶Ðµ Ð±Ð¸Ð¾ÑÐ¸Ð·Ð¸ÑÐµÑÐºÐ¸Ðµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° Ð½ÐµÐ¹ÑÐ¾Ð½Ð¾Ð². ÐÐ¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ ÑÐµÑÐµÐ· 30 Ð´Ð½ÐµÐ¹ Ð¿Ð¾ÑÐ»Ðµ Ð­Ð¡ Ð½Ð°Ð±Ð»ÑÐ´Ð°ÐµÑÑÑ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ðµ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð²Ð¾Ð·Ð±ÑÐ¶Ð´Ð°ÑÑÐµÐ¹ Ð½ÐµÐ¹ÑÐ¾ÑÑÐ°Ð½ÑÐ¼Ð¸ÑÑÐ¸Ð¸. ÐÑÑÐ²Ð»ÐµÐ½Ð¾ Ð·Ð½Ð°ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ðµ ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ðµ ÑÐ¸ÑÐ»Ð° ÑÐ¿Ð¾Ð½ÑÐ°Ð½Ð½ÑÑ ÑÐ¾Ð±ÑÑÐ¸Ð¹ Ð½Ð° Ð¿Ð¸ÑÐ°Ð¼Ð¸Ð´Ð½ÑÑ Ð½ÐµÐ¹ÑÐ¾Ð½Ð°Ñ Ð¿Ð¾Ð»Ñ Ð¡Ð1. ÐÑÐ¸ ÑÑÐ¾Ð¼ Ð±Ð¸Ð¾ÑÐ¸Ð·Ð¸ÑÐµÑÐºÐ¸Ðµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° Ð¿Ð¸ÑÐ°Ð¼Ð¸Ð´Ð½ÑÑ Ð½ÐµÐ¹ÑÐ¾Ð½Ð¾Ð² Ð¿Ð¾Ð»Ñ CA1 Ð½Ðµ Ð¸Ð·Ð¼ÐµÐ½ÑÐ»Ð¸ÑÑ. Ð¢Ð°Ðº Ð¶Ðµ Ð¾ÑÑÑÑÑÑÐ²Ð¾Ð²Ð°Ð»Ð¸ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ ÑÐ¾ÐºÐ¾Ð², Ð¾Ð¿Ð¾ÑÑÐµÐ´Ð¾Ð²Ð°Ð½Ð½ÑÑ Ð¿Ð¾ÑÑÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¸Ð¼Ð¸ NMDA-ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ°Ð¼Ð¸, Ð²ÑÐ·Ð²Ð°Ð½Ð½ÑÑ Ð²ÑÑÐ¾ÐºÐ¾ÑÐ°ÑÑÐ¾ÑÐ½Ð¾Ð¹ ÑÑÐ¸Ð¼ÑÐ»ÑÑÐ¸ÐµÐ¹ Ð² Ð¿Ð¸ÑÐ°Ð¼Ð¸Ð´Ð½ÑÑ Ð½ÐµÐ¹ÑÐ¾Ð½Ð°Ñ Ð¿Ð¾Ð»Ñ CA1. ÐÑÐ»Ð¾ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¾ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ðµ ÑÑÐ¾Ð²Ð½Ñ Ð´Ð¾Ð»Ð³Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð¹ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°ÑÐ¸Ð¸, Ð¿ÑÐ¸ ÑÑÐ¾Ð¼ Ñ Ð¶Ð¸Ð²Ð¾ÑÐ½ÑÑ Ð¿Ð¾ÑÐ»Ðµ Ð­Ð¡ Ð²ÑÑÐ²Ð»ÐµÐ½ Ð½Ð¾Ð²ÑÐ¹ NMDA-Ð½ÐµÐ·Ð°Ð²Ð¸ÑÐ¸Ð¼ÑÐ¹ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼ Ð¸Ð½Ð´ÑÐºÑÐ¸Ð¸ Ð´Ð¾Ð»Ð³Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð¹ ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°ÑÐ¸Ð¸, Ð¾Ð±ÑÑÐ»Ð¾Ð²Ð»ÐµÐ½Ð½ÑÐ¹ ÑÐ°Ð±Ð¾ÑÐ¾Ð¹ Ð¼ÐµÑÐ°Ð±Ð¾ÑÑÐ¾Ð¿Ð½ÑÑ Ð³Ð»ÑÑÐ°Ð¼Ð°ÑÐ½ÑÑ ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð² Ð¿ÐµÑÐ²Ð¾Ð¹ Ð³ÑÑÐ¿Ð¿Ñ Ð¿ÐµÑÐ²Ð¾Ð³Ð¾ Ð¿Ð¾Ð´ÑÐ¸Ð¿Ð° (mGluR1). Ð£ ÐºÑÑÑ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½Ð¾Ð¹ Ð³ÑÑÐ¿Ð¿Ñ Ð¿Ð»Ð°ÑÑÐ¸ÑÐ½Ð¾ÑÑÑ Ð¾Ð±ÑÑÐ»Ð¾Ð²Ð»ÐµÐ½Ð° ÑÐ°Ð±Ð¾ÑÐ¾Ð¹ NMDA-ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð². Ð ÑÐµÐ»Ð¾Ð¼ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÐµ Ð´Ð°Ð½Ð½ÑÐµ Ð¾ÑÑÐ°Ð¶Ð°ÑÑ Ð½ÐµÐ³Ð°ÑÐ¸Ð²Ð½Ð¾Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð¿Ð¸Ð»Ð¾ÐºÐ°ÑÐ¿Ð¸Ð½-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð­Ð¡ Ð½Ð° Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ñ ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð´Ð¾Ð»Ð³Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð¹ ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¿Ð»Ð°ÑÑÐ¸ÑÐ½Ð¾ÑÑÐ¸ Ð² Ð¿Ð¾Ð»Ðµ Ð¡Ð1 Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð° Ð²Ð·ÑÐ¾ÑÐ»ÑÑ ÐºÑÑÑ. ÐÐ°Ð»ÑÐ½ÐµÐ¹ÑÐ¸Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¿Ð¾ÑÑÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð¿ÑÐ¾ÑÐµÑÑÐ¾Ð² ÑÐ¸Ð³Ð½Ð°Ð»Ð¸Ð½Ð³Ð°, Ð¿ÑÐ¸Ð²Ð¾Ð´ÑÑÐ¸Ñ Ðº Â«Ð¿Ð¾Ð»Ð¾Ð¼ÐºÐµÂ» Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð° ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¿Ð»Ð°ÑÑÐ¸ÑÐ½Ð¾ÑÑÐ¸, Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÑ Ð¿Ð¾Ð½ÑÑÑ Ð¿ÑÐ¸ÑÐ¸Ð½Ñ Ð½Ð°Ð±Ð»ÑÐ´Ð°ÐµÐ¼ÑÑ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹, ÑÑÐ¾ Ð¿Ð¾Ð¼Ð¾Ð¶ÐµÑ ÑÐ¾ÑÑÐ°Ð²Ð¸ÑÑ ÑÑÑÐ°ÑÐµÐ³Ð¸Ð¸ Ð»ÐµÑÐµÐ½Ð¸Ñ Ð´Ð°Ð¶Ðµ Ð½Ð° Ð¿Ð¾Ð·Ð´Ð½Ð¸Ñ ÑÑÐ°Ð´Ð¸ÑÑ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¾Ð³ÐµÐ½ÐµÐ·Ð°.
description: The subject of the graduate qualification work is: "Changes in synaptic plasticity in the chronic phase of the lithium-pilocarpine model of temporal epilepsy". This work is devoted to the study of the molecular mechanisms of the formation of long-term synaptic plasticity in the lithium-pilocarpine model of temporal lobe epilepsy (TLE). Recent electrophysiological studies show that after convulsive states, its characteristics in the hippocampus changes. However, the reason of these changes and the mechanisms underlying them are still unclear. The purpose of this work was to study the mechanisms of induction of long-term synaptic potentiation in the CA1 field of the rat hippocampus in the chronic phase of the lithium-pilocarpine model of TLE. Tasks that were solved during the execution of the work: 1. To evaluate the characteristics of excitatory synaptic transmission in the hippocampal CA1 field. 2. To study changes in the biophysical properties of pyramidal neurons of CA1 Â hippocampal field. 3. To explore the properties of long-term synaptic potentiation (TLP). 4. To study the role of NMDA receptors in the formation of LTP in CA1 field. 5. To investigate the characteristics of currents flowing through NMDA receptors mediated by high-frequency stimulation on pyramidal neurons in the hippocampal CA1 field. 6. To study the contribution of metabotropic glutamate receptors of type I mGluR1 to the induction of LTP in the hippocampal CA1 field. The work was carried out on the basis of the I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry of the Russian Academy of Sciences in the Laboratory of Molecular Mechanisms of Neural Interactions. The study was performed on male Wistar rats 30 days after pilocarpine-induced status epilepticus (SE). For electrophysiological studies, a technique was used to divert field exciting postsynaptic potentials from the radial layer of the hippocampal CA1 field. LTP was caused by high-frequency stimulation. Using the patch-clamp method in the "whole cell" mode, NMDA-mediated currents in the pyramidal neurons of the hippocampus, as well as the biophysical properties of neurons, were studied.It is shown that 30 days after SE, there is a decrease in the effectiveness of excitatory neurotransmission. A significant increase in the number of spontaneous events on pyramidal neurons of the CA1 field was revealed. At the same time, the biophysical properties of the pyramidal neurons of the CA1 field does not change. There were also no changes in currents mediated by postsynaptic NMDA receptors caused by high-frequency stimulation in pyramidal neurons of the CA1 field. There was the decrease in the level of long-term potentiation, while a new NMDA-independent mechanism of induction of LTP was revealed in animals after SE, due to the work of metabotropic glutamate receptors of the first group of the first subtype (mGluR1). In rats of the control group, plasticity is due to the work of NMDA receptors.In general, this study shows the negative effect of pilocarpine-induced SE on the mechanisms of formation of long-term synaptic plasticity in the CA1 field of the hippocampus of adult rats. Further studies of postsynaptic signaling processes leading to the "breakdown" of the synaptic plasticity mechanism will help to understand the cause of the observed changes, which will help to formulate treatment strategies even at the late stages of epileptogenesis.