cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Recovery of rare cells and single cells analysis: different opportunities and challenging applications
Creators: name: MAIOCCHI, REBECCA
Publisher: name: Universit� degli Studi di Siena
Year: 2021

description: Single-cell biology is a new discipline which aims to address and solve the problem of cellular
heterogeneity. Single-cell omic, which allows the molecular investigation of different cell types in a
high throughput manner, is driving the Precision and Personalized Medicine approaches.
Single B cell isolation strategies, the starting points of the single-cell omics, have become essential
research procedures for efficiently sampling the natural repertoire of immunized animals and
humans (Tiller et al, 2008). The goal of the first study is to demonstrate the feasibility of a novel
approach to rapidly generate recombinant mAbs recovering rare antigen-specific plasma cells
from complex samples derived from immunized mice. TLS Foundation, where this work has been
carried out, has recently invested in Precision Medicine activities and, in particular, in the
development of an alternative method to hybridoma technology to isolate rare antigen-specific B
cells from blood of immunized or infected individuals. In Study 1 we have set-up a FACS sorter
free method for a fast identification and isolation of antigen-specific plasma cells producing IgG
with unique and desirable features and, moreover, we have obtained high yield TAP-PCR products.
This could simplify the actual hybridoma technology procedure for the identification and
molecular cloning of antigen-specific antibodies from single-B cells.
Single-cell biology is also used to address and solve problems related to tumour heterogeneity.
Cancer is one of the research areas that has greatly benefited from single-cell analysis. The
complexity of immune responses to cancer has hampered the development of novel therapeutical
approaches with the exception of monoclonal antibody based-therapies that target specific
immunomodulators. In the last 10 years PD-1 blockade monoclonal therapy has indeed
revolutionized cancer treatments but a substantial population of patients is still unresponsive. To
rescue unresponsive patients, the mechanism of unresponsiveness and phenotype must be
elucidated. The second part of this work (Study 2) deals with the possibility to reveal one of the
mechanism responsible for T cells exhaustion in patients that are non-responsive to monoclonal
anti-PD1 therapy. We demonstrated that there is a correlation between the overexpression of TFX
(Transcriptional Factor X, the real name of the factor has been hidden for confidentiality) and the
onset of the characteristic features of exhaustion. Indeed the KO of this gene restores in T cells
their functionality through a more active metabolism. Further in-depth study of the modulation of
this transcription factor may elucidate the pathways and the genes responsible for the exhaustion
phenotype in T cells.
What determines FinTech success?—A taxonomy-based analysis of FinTech success factors
Creators: name: Werth, Oliver
name: Cardona Davinia Rodríguez
name: Torno, Albert
name: Breitner, Michael H.
name: Muntermann, Jan
Publisher: name: Berlin ; Heidelberg : Springer
Year: 2023
Subjects: subject: FinTech business models
subject: FinTech success factors
subject: Taxonomy-based analysis
subject: Dewey Decimal Classification::600 | Technik::620 | Ingenieurwissenschaften und Maschinenbau

description: Value creation in the financial services sector has been fundamentally transformed by digitally born financial technology (FinTech) companies. FinTech companies synthesize information systems with financial services. Given its disruptive power, the FinTech phenomenon has received great attention in academic research, practice, and media. Still, limited systematic research provides a structure and holistic view of FinTechs’ success. Aiming to enhance understanding of the factors enabling FinTech success, we classify success factors across extant scientific literature on distinct FinTech business model archetypes. Our analysis reveals that the “cost–benefit dynamic of the innovation,” “technology adoption,” “security, privacy, and transparency,” “user trust,” “user-perceived quality,” and “industry rivalry” are crucial factors for FinTech success and can be seen as “grand challenges” for the FinTech ecosystem. In addition, we validate and discuss our findings with real-world examples from the FinTech industry and two interviews with stakeholders from the FinTech ecosystem. Our study contributes to the knowledge of FinTechs by providing a classification system of success factors for practitioners and researchers.
Additional file 2 of The efficacy and safety of apatinib plus capecitabine in platinum-refractory metastatic and/or recurrent nasopharyngeal carcinoma: a prospective, phase II trial
Creators: name: Tang, Lin-Quan
name: Li, Xiao-Yun
name: Li, Zhi-Ming
name: Liu, Zhi-Gang
name: Lin, Miao-Zhen
name: Zhou, Huan
name: Yu, Qi-Wen
name: Zhou, Jian
name: Zhao, Chong
name: Chen, Ze-Bin
name: Wang, Xi-Cheng
name: Peng, Jia-Yu
name: Chen, Qiu-Yan
name: Fang, Wen-Feng
name: Yang, Yun-Peng
name: Zhang, Bei
name: Xia, Liang-Ping
name: Hu, Pi-Li
name: Hu, Wei-Han
name: Li, Yi-Jie
name: Mai, Hai-Qiang
name: Cai, Xiu-Yu
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Environmental Sciences not elsewhere classified
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Science Policy
subject: Infectious Diseases
subject: FOS: Health sciences

description: Additional file 2: Figures S3-S5. Fig S3- Dose reduction of experimental drugs. Fig S4- Main causes for reduction of apatinib. Fig S5- Main causes for reduction of capecitabine.
Density fractionation reworked: Reduce material and costs
Creators: name: Liebmann, Patrick
name: Mewes, Ole
name: Guggenberger, Georg
Publisher: name: Weinheim : Wiley-VCH
Year: 2023
Subjects: subject: mineral-associated organic matter
subject: particulate organic matter
subject: sodium polytungstate
subject: soil fractions
subject: soil organic matter
subject: Dewey Decimal Classification::500 | Naturwissenschaften::580 | Pflanzen (Botanik)
subject: Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
subject: Dewey Decimal Classification::600 | Technik::630 | Landwirtschaft, Veterinärmedizin
subject: Dewey Decimal Classification::600 | Technik::640 | Hauswirtschaft und Familienleben

description: Soil density fractionation is a common tool to separate organic matter of different function and turnover. But it has not been tested so far how much soil material is necessary to obtain reproducible results. A reduction of chemicals like polytungstate would further save valuable resources. Here, we show that soil weight reduction from 25 to 5 g was not significantly affecting fractionation results. Compared to the commonly used 10–25 g, this corresponds to a saving of resources of up to 80%.
Parametric study on contribution of combined confining steel and steel fiber to column’s displacement ductility
Creators: name: Slamet Widodo
name: Bambang Sabariman
name: Tavio
Publisher: name: Faculty of Engineering, Khon Kaen University
Year: 2024
Subjects: Confinement
Disaster risk reduction
Drift ratio
Ductility
Quasi-cyclic
Steel fiber

description: Engineering and Applied Science Research, 51, 4, 473-481
THE SIZE BIAS: DOES IT EXIST, AND HOW WOULD WE EXAMINE IT IN THE BRAIN
Creators: name: Larranaga, Daniel Lucas
Publisher: name: Purdue University Graduate School
Year: 2021
Subjects: Psychophysiology
Psycholinguistics (incl. speech production and comprehension)
Cognitive neuroscience

description: Abstract: Many regions of the cortex have been identified to be specifically selective for different features. For example, visually presented stimuli proceed, via both the dorsal “where” and ventral “what” streams, before converging in the frontal cortex for decision making processes. However, several subregions of both streams have been identified that demonstrate selectivity on many semantic dimensions, such as size. Most of the studies examining regions selectively activated in maintenance of semantic size have employed the use of visually presented images. In the present, however, study we provide a review of relevant literature, proposed techniques, and a list of word stimuli that may help elucidate the multivariate neural processing of several semantic dimensions.
Additional file 1 of The efficacy and safety of apatinib plus capecitabine in platinum-refractory metastatic and/or recurrent nasopharyngeal carcinoma: a prospective, phase II trial
Creators: name: Tang, Lin-Quan
name: Li, Xiao-Yun
name: Li, Zhi-Ming
name: Liu, Zhi-Gang
name: Lin, Miao-Zhen
name: Zhou, Huan
name: Yu, Qi-Wen
name: Zhou, Jian
name: Zhao, Chong
name: Chen, Ze-Bin
name: Wang, Xi-Cheng
name: Peng, Jia-Yu
name: Chen, Qiu-Yan
name: Fang, Wen-Feng
name: Yang, Yun-Peng
name: Zhang, Bei
name: Xia, Liang-Ping
name: Hu, Pi-Li
name: Hu, Wei-Han
name: Li, Yi-Jie
name: Mai, Hai-Qiang
name: Cai, Xiu-Yu
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Environmental Sciences not elsewhere classified
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Science Policy
subject: Infectious Diseases
subject: FOS: Health sciences

description: Additional file 1: Figures S1-S2. Fig.S1-Serial computed tomography scans showing the appearance of the target lesions before treatment. Fig.S2-Serial computed tomography scans showing the appearance of the target lesions after 6 weeks (two cycles) of treatment.
Studio dell'influenza genotipo-ambiente sulle caratteristiche qualitative dell'olio vergine di oliva (Olea europea L.)
Creators: name: TURA, DEBORA
Publisher: name: Universit� degli Studi di Milano
Year: 2004

description: The quality of virgin olive oil depends on cultivar, environment, orchard management, oil extraction and storage technology. The aim of this PhD project was to investigate the cultivar and environmental influences on virgin olive oil quality during 4 years (1998-2001). The first goal (cultivar influence) was divided in three aspects: chemiometric (61 oils from 18 cultivars), enzymatic (22 olive samples from 3 cultivars at different ripening stages) and aromatic (40 commercial oils). The second goal (environmental influence) was divided in two parts: mesoclimate effect (77 oils of 4 cultivars from 17 orchards in western cost of lake Garda, northern Italy) and macroclimate effect (33 oils of 3 cultivars at three ripening stages from different Italian regions). The analytical determinations on oils were: fatty acids, triglycerides, aliphatic and triterpenic alcohols, sterols, polyphenols, tocopherols, pigments, volatile compounds, oxidative stability and sensory analysis. The cultivar resulted the most important factor influencing the oil quality, while the environment was less important in influencing the oil orchard origin in Garda area, whereas it was more significant in separating the cultivar and the origin from different region. Some cultivars were similar according to chemical profile, whereas others resulted well distinct and characterized. In addition, the oxidative stability was correlated to total polyphenols, saturated on polyunsaturated fatty acids ratio and total tocopherols.
Structural equation modeling of the factors influencing pedestrians’ overpass utilization preference: A case study in Iligan City, Philippines
Creators: name: Opon, Joel G.
name: Frexie L. Unde
name: Oliva, Kyle Adrian A.
name: Bas, Augustus Nicko T.
name: Masalig, Raquel O.
name: Cheery May S. Florendo
name: Florife D. Liwanag
name: Rengie P. Bagares
Publisher: name: Faculty of Engineering, Khon Kaen University
Year: 2024
Subjects: Overpass
Utilization
Latent factors
Exploratory factor analysis
Structural equation modeling

description: Engineering and Applied Science Research, 51, 4, 482-494
LA PROCREAZIONE MEDICALMENTE ASSISTITA AL BANCO DI PROVA DEL GIUDICE NAZIONALE E SOVRANAZIONALE
Creators: name: VERRI, ALESSANDRA
Publisher: name: Universit� degli Studi di Milano
Year: 2014

description: Scopo del presente lavoro � analizzare in modo critico la legge n. 40/2004, evidenziandone le numerose lacune ed imprecisioni tecniche, e soprattutto i suoi diversi profili di illegittimit� costituzionale (in particolare sotto il profilo del principio di laicit�, ragionevolezza e del diritto alla salute), nonch� il rapporto fra essa e la disciplina sull?aborto, stabilendo altres� un confronto con le norme presenti nei principali Paesi europei.
In particolare vengono affrontati tre temi. Il primo riguarda l?originario divieto di produrre per ogni ciclo di trattamento di PMA un numero di embrioni superiore a tre, nonch� l?obbligo di trasferire contemporaneamente tutti gli embrioni prodotti, a cui era originariamente correlato il divieto di crioconservazione e di soppressione degli embrioni prodotti; il secondo riguarda l?implicito divieto di effettuare la diagnosi genetica preimpianto, che � stato per� recentemente rimosso grazie all?intervento di giudici sovranazionali e nazionali; e l?ultimo il divieto, tuttora esistente, di praticare forme di procreazione di tipo eterologo.
Per ciascuno di essi vengono messi in luce i diversi profili di illegittimit� costituzionale, evidenziando, in particolare, come nel corso degli ultimi anni alcuni di essi siano stati corretti grazie alle diverse pronunce della giurisprudenza nazionale e sovranazionale, mentre altri siano rimasti pressoch� immutati, ed esaminando infine quali prospettive vi siano per rimediarvi.
Incorporation and Deposition of Spin Crossover Materials into and onto Electrospun Nanofibers
Creators: name: Kilic, Maximilian Seydi
name: Brehme, Jules
name: Pawlak, Justus
name: Tran, Kevin
name: Bauer, Friedrich Wilhelm
name: Shiga, Takuya
name: Suzuki, Taisei
name: Nihei, Masayuki
name: Sindelar, Ralf Franz
name: Renz, Franz
Publisher: name: Basel : MDPI
Year: 2023
Subjects: subject: (coaxial)-electrospinning
subject: composites
subject: coordination chemistry
subject: nano fibers
subject: PMMA
subject: spin crossover
subject: triazole complexes
subject: Dewey Decimal Classification::500 | Naturwissenschaften::540 | Chemie

description: We synthesized iron(II)-triazole spin crossover compounds of the type [Fe(atrz)3]X2 and incorporated and deposited them on electrospun polymer nanofibers. For this, we used two separate electrospinning methods with the goal of obtaining polymer complex composites with intact switching properties. In view of possible applications, we chose iron(II)-triazole-complexes that are known to exhibit spin crossover close to ambient temperature. Therefore, we used the complexes [Fe(atrz)3]Cl2 and [Fe(atrz)3](2ns)2 (2ns = 2-Naphthalenesulfonate) and deposited those on fibers of polymethylmethacrylate (PMMA) and incorporated them into core–shell-like PMMA fiber structures. These core–shell structures showed to be inert to outer environmental influences, such as droplets of water, which we purposely cast on the fiber structure, and it did not rinse away the used complex. We analyzed both the complexes and the composites with IR-, UV/Vis, Mössbauer spectroscopy, SQUID magnetometry, as well as SEM and EDX imaging. The analysis via UV/Vis spectroscopy, Mössbauer spectroscopy, and temperature-dependent magnetic measurements with the SQUID magnetometer showed that the spin crossover properties were maintained and were not changed after the electrospinning processes.
The paroxysmal disorder gene PRRT2 downregulates NaV channels and neuronal excitability in human neurons
Creators: name: GIANSANTE, GIORGIA
Publisher: name: Universit� degli studi di Genova
Year: 2018

description: Proline-Rich Transmembrane Protein 2 (PRRT2) has been identified as the single causative gene for a group of paroxysmal syndromes, including benign familial infantile seizures, paroxysmal kinesigenic dyskinesia and migraine. Most of the mutations of this gene lead to a premature stop codon, generating an unstable form of mRNA or a truncated protein that is degraded, pointing out the loss of the PRRT2 function as pathogenic mechanism of action. In this thesis, we have used different approaches to investigate the pathophysiological function of PRRT2. An important role for PRRT2 in the neurotransmitter release machinery, brain development and synapse formation has been uncovered by a previous work performed in our laboratory by acute silencing of PRRT2 expression. Here, we analyzed the phenotype of primary hippocampal neurons obtained from mouse PRRT2 knockout (KO) embryos. Analysis of synaptic function in primary neurons obtained from PRRT2-KO showed a largely similar, albeit attenuated, synaptic phenotype with respect to acute PRRT2 silencing characterized by weakened spontaneous/evoked synaptic transmission and increased facilitation at excitatory synapses. These effects were accompanied by a strengthened inhibitory transmission that, however, displayed faster synaptic depression. At the network level, these synaptic phenotypes, resulted in a state of increased spontaneous and evoked neurotransmitter release with increased excitability of excitatory neurons. To better dissect the physiological role of PRRT2, we characterized the phenotypes of neurons differentiated from Induced Pluripotent Stem Cells (iPSCs) from patients homozygous for the PRRT2 c.649dupC mutation. Hence, we observed an increased Na+ current and firing activity in iPSCs rescued with the re-expression of the human wild-type form of PRRT2. By use of heterologous expression system, we demonstrate that PRRT2 interacts with NaV1.2/NaV1.6, but not with NaV1.1 channels, modulating their membrane exposure and decreasing their conductances. In brief, our findings highlighted that PRRT2 mutations might be a negative modulator of NaV1.2/NaV1.6 channels and point out the critical role of this protein in the regulation of the neuronal network functionality.
sj-pdf-1-cmx-10.1177_10775595211041974 – Supplemental Material for Child Abuse Pediatricians Assess a Low Likelihood of Abuse in Half of 2890 Physical Abuse Consults
Creators: name: Johnson, Katie L.
name: Brown, Emily C. B.
name: Feldman, Kenneth W.
name: Qu, Pingping
name: Lindberg, Daniel M.
Publisher: name: SAGE Journals
Year: 2021
Subjects: subject: 160299 Criminology not elsewhere classified
subject: FOS: Law

description: Supplemental Material, sj-pdf-1-cmx-10.1177_10775595211041974 for Child Abuse Pediatricians Assess a Low Likelihood of Abuse in Half of 2890 Physical Abuse Consults by Katie L. Johnson, Emily C. B. Brown, Kenneth W. Feldman, Pingping Qu, Daniel M. Lindberg and for the ExSTRA Investigators in Child Maltreatment
Influence of prickly pear gum on the physical and mechanical properties of adobe reinforced with palm fiber
Creators: name: Socrates Pedro Muñoz Pérez
name: Jeiner Rubio Guevara
name: Perales, José Francisco Ruiz
name: Chumacero, Juan Martín García
name: Diaz, Elver Sanchez
name: Llatas, Flor Delicia Heredia
name: Brast, Carlos Eduardo Ramos
name: Coronel, Álvaro Andres Calderón
name: Perales, Miguel Angel Ruiz
Publisher: name: Faculty of Engineering, Khon Kaen University
Year: 2024
Subjects: Adobe
Climatic conditions and changes
Prickly pear gum
Palm fiber
Mechanical properties

description: Engineering and Applied Science Research, 51, 4, 495-504
Immune modulatory effects of novel monoclonal antibodies target therapies in severe eosinophilic asthma patients
Creators: name: BERGANTINI, LAURA
Publisher: name: Universit� degli Studi di Siena
Year: 2020

description: New anti IL-5 antibodies, mepolizumab and benralizumab, have recently been approved for severe asthma, sharing the same inclusion criteria. To contribute on biomarkers research leading to the personalized choice, we investigated L-selectin, KL-6 and lymphocyte subsets as bioindicators of airways hyper-responsiveness and remodelling and to phenotype patients according to their answer to the treatment. L?selectin mediates leukocyte rolling of lung endothelium and its expression on T cells is increased in asthma patients. Regulatory T cells (Tregs) suppress inflammation by secreting a wide variety of cytokines that inhibit T cell proliferation.
A cohort of 28 patients affected by severe eosinophilic asthma were treated with anti IL-5 drugs: 20 with Mepolizumab and 8 with Benralizumab. Lymphocytes subsets, Regulatory T cells and CD4+CD62L+ cells were analyzed through flow cytometry, Serum L-selectin quantification was performed by bead-based multiplex analysis, while KL-6 was analyzed through CLEIA.
Clinical, functional and immunological data at baseline (T0), after one month (T1) and 6 months of therapy were collected in a database. All treated patients showed variations in FEV1, FEV1/FVC ratio and peripheral eosinophils for both drugs. Mepolizumab treated patients also showed significant differences between T0 and T1 in CD8+ and NK-T like cells percentages and a significant increase in L-selectin concentrations. Stratifying the cohort of our patients in ?early responders and partial responders at T0 they showed significant differences in peripheral eosinophils, sL-selectin, and KL-6, while no differences were found at T0 between ?early responders? and ?partial responders? patients treated with Benralizumab. In a subgroup of 14 mepolizumab treated patients, immunological data showed an increase in Tregs and CD4+CD62L+ at T1. Soluble L-selectin concentrations were lower at T1. CD45+ and CD62L+ cell features differed significantly in early and partial responders before and after therapy. The FEV1/FVC ratio showed an indirect correlation with L-selectin levels (r=-0.6, p=0.03), peripheral eosinophilia (r=-0.7, p=0.01). This real-life study provides new insights for the personalized approach to severe asthma therapy. Although preliminary, the results indicate that besides to peripheral eosinophils, other molecules are useful as biomarkers of early response that can also involving in the pathogenesis of severe asthma. Mepolizumab therapy was found to modulate immune response, restoring immune balance in patients with SEA. L-selectin and Tregs were also proposed as biomarkers of response to mepolizumab treatment.
sj-docx-1-hpp-10.1177_15248399211028154 – Supplemental material for Integrating Safety and Health Promotion in Workplaces: A Scoping Review of Facilitators, Barriers, and Recommendations
Creators: name: Biswas, Aviroop
name: Begum, Momtaz
name: Van Eerd, Dwayne
name: Johnston, Heather
name: Smith, Peter M.
name: Gignac, Monique A. M.
Publisher: name: SAGE Journals
Year: 2021
Subjects: subject: 111708 Health and Community Services
subject: FOS: Health sciences
subject: 111799 Public Health and Health Services not elsewhere classified
subject: Sociology
subject: FOS: Sociology

description: Supplemental material, sj-docx-1-hpp-10.1177_15248399211028154 for Integrating Safety and Health Promotion in Workplaces: A Scoping Review of Facilitators, Barriers, and Recommendations by Aviroop Biswas, Momtaz Begum, Dwayne Van Eerd, Heather Johnston, Peter M. Smith and Monique A. M. Gignac in Health Promotion Practice
Microstructural and mechanical behavior of friction stir welded AZ31-AZ61 magnesium alloys dissimilar joint
Creators: name: Kellai, Ahmed
name: Liamine Kaba
name: Seddik Ouallam
name: Dehimi, Said
name: Kahla, Sami
name: Djeghlal, Mohammed Elamine
name: Bendris, Tarek
Publisher: name: Faculty of Engineering, Khon Kaen University
Year: 2024
Subjects: Friction stir welding
Magnesium alloy
Intermetallic compounds
Microstructure
Mechanical property

description: Engineering and Applied Science Research, 51, 4, 505-515
Embodied Narration and The Body as Story in Processes of Knowledge Production
Creators: name: Conrad, Karissa Michele
Publisher: name: Purdue University Graduate School
Year: 2021
Subjects: Communication studies
Organisational, interpersonal and intercultural communication

description: This project proposes, develops, and tests a new theoretical concept termed embodied narration to understand the role of the body in processes of knowledge production. Specifically, this project draws upon postmodern organizing and narrative theorizing to argue that the body itself – not just those stories told about the body – is discursive, and thus, serves to produce, reproduce, or challenge existing organizational knowledge and power structures. Embodied narration – the expression of the body’s stories through the body without the imposition of the written or spoken word – is conceptualized as one process through which such continued oppression or resistance occurs.
Embodied narration was examined within the organizing context of distance running, a fitting context given its operation as an ideological discipline centered on embodied practices and the salience of the gaze among organizational members. This study employed photovoice methodology to procure participant photographs meant to approximate spectatorship of embodied narration. Further, participants shared their experiences and observations of organizational knowledge and disciplinary mechanisms in semi-structured interviews. A grounded theory analysis was employed to identify emergent themes related to organizational knowledge and discipline and provided the researcher and other readers with the preunderstanding of organizational knowledge necessary for situated interpretations of photographs. Participant photographs are presented in the absence of the written or spoken word to allow the subjects depicted in them to speak for themselves, as is consistent with the assumptions of embodied narration.
Results indicate unique knowledge and disciplinary mechanisms within the organizing context of distance running which are consistent with past and ongoing research. Importantly, participant stories of spectatorship provide insight into the possibilities of embodied narration within this context, including the role of member inclusion/exclusion and body visibility/invisibility in such processes. From these stories, conclusions were drawn regarding the many possible ways by which embodied narration may be employed or enacted within other contexts. In all, this project extends postmodern organizing and narrative theorizing by rejecting the assumption that discourse is inherently linguistic, and by introducing a new process by which power/knowledge is produced.
Jörg Kärger: Diffusion Is His Life
Creators: name: Caro, Jürgen
Publisher: name: Weinheim : Wiley-VCH Verl.
Year: 2023
Subjects: subject: Diffusion
subject: Membranes
subject: Metal-organic frameworks
subject: Nanoporous materials
subject: Nuclear magnetic resonance
subject: Zeolites
subject: Dewey Decimal Classification::500 | Naturwissenschaften::540 | Chemie
subject: Dewey Decimal Classification::600 | Technik::660 | Technische Chemie

description: Jörg Kärger is a pioneer in the field of diffusion and his work has contributed significantly to the understanding of molecular mass transfer in porous systems. He has been actively researching diffusion in nanoporous materials for more than 50 years and, according to the Web of Science, is the most productive author in the field of diffusion, with 247 entries (search for diffusion in the title). He also has the highest total local citation score in the field of diffusion, which indicates not only the high number but also the high quality of his publications.
Application of computational methods for the design and optimization of Src family kinase inhibitors
Creators: name: PASSANNANTI, RAFFAELE
Publisher: name: Universit� degli Studi di Siena
Year: 2021

description: Kinases constitute a family of enzymes involved in many cellular signalling pathways thanks to their ability to catalyse the transfer of a phosphate group from a donor, ATP or GTP, to an acceptor, a protein or a lipid. Src family kinases (SFKs), that represent the topic of this PhD work, belong to the cytosolic tyrosine kinases group and act by transferring the phosphate group to a tyrosine of the acceptor protein. SFKs play a key role in controlling cellular processes such as proliferation, apoptosis, angiogenesis, and invasion. Hyperactivation of SFKs was observed in many human cancers, which makes these enzymes very attractive targets for the treatment of these diseases.
In this PhD work, the application of molecular modelling techniques allowed the design and optimization of SFK inhibitors. In a first project, the ability of the pyrazolo[3,4-d]pyrimidine ALM4-23 to stabilize c-Src in the three different conformational states, was evaluated by computational techniques. The resulting insights were used to design ALM4-23 derivatives with potentially improved inhibitory activity against c-Src. In a second project, a structure-based virtual screening procedure was applied to a database of commercially available compounds to identify new c-Src inhibitors. The 3D structure of the kinase in an inactive conformation was used because potential advantages in terms of selectivity and drug residence time. In the last project, the pyrazolo[3,4-d]pyrimidine Fyn inhibitor, Si308, was studied to evaluate its ability to inhibit other SFK members, such as Lyn and Blk. Once confirmed the ability of Si308 to act as a multitarget SFKs inhibitor, a library of its derivatives was synthesized and tested against B- and T-cell lymphoma cell lines, in which Fyn, Lyn and Blk play a pivotal role.
sj-docx-2-mpp-10.1177_23814683211045659 – Supplemental material for Risk Communication After Health Care Exposures: An Experimental Vignette Survey With Patients
Creators: name: Elwy, A. Rani
name: Maguire, Elizabeth M.
name: Gallagher, Thomas H.
name: Asch, Steven M.
name: Durfee, Janet M.
name: Martinello, Richard A.
name: Bokhour, Barbara G.
name: Gifford, Allen L.
name: Taylor, Thomas J.
name: Wagner, Todd H.
Publisher: name: SAGE Journals
Year: 2021
Subjects: subject: 111799 Public Health and Health Services not elsewhere classified
subject: FOS: Health sciences

description: Supplemental material, sj-docx-2-mpp-10.1177_23814683211045659 for Risk Communication After Health Care Exposures: An Experimental Vignette Survey With Patients by A. Rani Elwy, Elizabeth M. Maguire, Thomas H. Gallagher, Steven M. Asch, Janet M. Durfee, Richard A. Martinello, Barbara G. Bokhour, Allen L. Gifford, Thomas J. Taylor and Todd H. Wagner in MDM Policy & Practice
Application of strategic environmental assessment for mass transit system: Case study of Uttaradit Province, Thailand
Creators: name: Pitsanu Pannaracha
name: Dondej Tungtakanpoung
Publisher: name: Faculty of Engineering, Khon Kaen University
Year: 2024
Subjects: SEA
Mass transit system
Indicators
Alternatives
Pairwise comparison
Impact matrix analysis

description: Engineering and Applied Science Research, 51, 4, 516-523
Integral Fourier transforms and the integral Hodge conjecture for one-cycles on abelian varieties
Creators: name: Beckmann, Thorsten
name: De Gaay Fortman, Olivier
Publisher: name: Cambridge : Cambridge Univ. Press
Year: 2023
Subjects: subject: Chow rings
subject: cohomology
subject: integral Hodge conjecture
subject: subvarieties of abelian varieties
subject: Dewey Decimal Classification::500 | Naturwissenschaften::510 | Mathematik

description: We prove the integral Hodge conjecture for one-cycles on a principally polarized complex abelian variety whose minimal class is algebraic. In particular, the Jacobian of a smooth projective curve over the complex numbers satisfies the integral Hodge conjecture for one-cycles. The main ingredient is a lift of the Fourier transform to integral Chow groups. Similarly, we prove the integral Tate conjecture for one-cycles on the Jacobian of a smooth projective curve over the separable closure of a finitely generated field. Furthermore, abelian varieties satisfying such a conjecture are dense in their moduli space.
Additional file 1 of Resistance training's impact on blood biomarkers and cognitive function in older adults with low and high risk of mild cognitive impairment: a randomized controlled trial
Creators: name: Vints, Wouter A. J.
name: Gökçe, Evrim
name: Šeikinaitė, Julija
name: Kušleikienė, Simona
name: Česnaitienė, Vida J.
name: Verbunt, Jeanine
name: Levin, Oron
name: Masiulis, Nerijus
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Cell Biology
subject: Neuroscience
subject: Physiology
subject: FOS: Biological sciences
subject: Sociology
subject: FOS: Sociology
subject: Mental Health

description: Additional file 1: Supplementary Table 1. Bivariate correlations between changes in blood biomarkers and cognition in both experimental and control group. Supplementary Fig. 1. Bivariate relationship between pre-to-post changes in IL-6 and ANAM Memory search response time (ms) in both experimental and control group.
STUDIO BIOCHIMICO E FARMACOLOGICO DI CELLULE MUSCOLARI LISCE ISOLATE DA TESSUTI DI PAZIENTI AFFETTI DA TSC2 E LAM. MODIFICAZIONI EPIGENETICHE DEL GENE TSC2 NELLA PATOGENENESI DELLA TSC E DELLA LAM.
Creators: name: ANCONA, SILVIA
Publisher: name: Universit� degli Studi di Milano
Year: 2011

description: Tuberous sclerosis complex (TSC) and lymphangioleiomymatosis (LAM) are rare diseases. TSC is a genetic disease caused by mutation in TSC1 or TSC2 genes. TSC2 cells form hamartomas and might invade lungs causing the fatal diseases LAM. From an angiomylipoma (AML) of a TSC male patient we isolated a TSC2 smooth muscle (ASM) population. The growth of these cells was EGF-dependent. They showed a constitutive S6 phosphorylation, and lacked tuberin as the result of TSC2 promoter methylation (TSC2-/meth ASM cells). Chromatin remodeling agents, such as trichostatin?A and 5-azacytidine de-methylated TSC2-/meth ASM cell promoter and induced tuberin expression. The blockade of EGF-receptor with specific antibodies results in cell death as shown in TSC2-/- ASM cells, a population previously isolated from an AML of female TSC patient. LAM cells migrate or metastasize to other organs, in fact cells with TSC2 mutation have been found in AMLs and lung lesions of LAM patients. We isolated a population from chylous of a TSC/LAM patient, in which, as in TSC2-/meth cells, tuberin expression was induced by 5-azacytidine and trichostatin A treatments. These cells required the supplementation of EGF for proliferation, such as TSC2-/- and TSC2-/meth ASM cells. Chylous TSC/LAM cells expressed marker for identification of mesenchimal characteristics, such as vimentin and SNAIL, while E-cadherin, usually not expressed in metastatized cancer cells, was not detectable. The acquisition of mesenchymal characteristics for cancer cells is a transient event that might be important for migration and tissue invasion. Chylous TSC/LAM cells switched from a short floating stage, with low S6 phosphorylation levels, to a stage of adhesion with high S6 phosphorylation. These data suggest a novel therapeutic approach for the control of TSC and LAM abnormal cell growth using anti-EGFR antibody in addition to rapamycin, and, in some cases, the chromatin remodelling agents. Moreover, our data confirm the evidence that TSC2 pathogenesis might originate from an epigenetic defect.
STRUCTURAL CHARACTERIZATION OF THE EARLY STAGES OF THE BETA 2 MICROGLOBULIN AMYLOIDOSIS
Creators: name: COLOMBO, MATTEO MARIA
Publisher: name: Universit� degli Studi di Milano
Year: 2012

description: ABSTRACT.
Background.
?-2 microglobulin (?2m) is an amyloidogenic protein responsible for dialysis related amyloidosis in man, which result in the deposition of ?2m amyloid fibrils at a skeletal level. ?2m is a 99 residue protein formed by two ?-sheets linked by a disulphide bond. In the early stages of fibril formation, ?2m associate into dimers and higher order oligomers that are structurally poorly characterized due to their transient nature. Furthermore, the aggregation properties of ?2m are affected by its fold-stability. In particular, the DE loop region, which connect the D- and the E- strands, has been reported to be crucial for the ?2m fold-stability.

Results.
Four monomeric ?2m cysteine mutants (S20C, E50C, W60C and S88C) were produced and their correspondent disulphide-linked homodimers were prepared (DIMC20, DIMC50, DIMC60 and DIMC88). The aggregation properties, the crystallogenesis and the oligomerisation state in solution were tested for each ?2m homodimer. DIMC20, DIMC50 and DIMC88 form amyloid fibrils, crystals and display a varying mixtures of dimeric and tetrameric species in solution, while DIMC60 is not amyloidogenic and is purely dimeric in solution. DIMC20 and DIMC50 X-ray structures (2.45 � and 2.7 � resolution, respectively) shared a non-covalent D-D strand interface that mediate the formation of a tetrameric assembly in both DIMC20 and DIMC50. Moreover, DIMC20 and DIMC50 in solution can catalyse the w.t. ?2m fibrils formation in the absence of fibril seeds at pH 7.4, strongly suggesting that the D-D strand interface is involved in the early stages of ?2m amyloid aggregation.
In order to further characterize the role of the DE loop in ?2m fold-stability, a K58P-W60G ?2m mutant was produced and purified. The K58P-W60G ?2m mutant showed improved thermal and chemical stability and a faster folding compared to the w.t. ?2m. The crystal structure of the K58P-W60G ?2m mutant (1.25 � resolution) showed that the internal disulphide bond was severed as reported by Electrospray ionization-mass spectrometry spectra, which display that a fraction of the K58P-W60G ?2m mutant has a reduced disulphide bond. These data suggest a stabilizing role of Pro58 and stress the importance of the DE loop on the biophysical properties of the ?2m.
sj-docx-1-anr-10.1177_20530196211046036 – Supplemental material for Siliceous algae response to the “Great Acceleration” of the mid-20th century in Crawford Lake (Ontario, Canada): A potential candidate for the Anthropocene GSSP
Creators: name: Gushulak, Cale AC
name: Marshall, Matthew
name: Cumming, Brian F
name: Llew-Williams, Brendan
name: Timothy Patterson, R
name: McCarthy, Francine MG
Publisher: name: SAGE Journals
Year: 2021
Subjects: Geography

description: Supplemental material, sj-docx-1-anr-10.1177_20530196211046036 for Siliceous algae response to the “Great Acceleration” of the mid-20th century in Crawford Lake (Ontario, Canada): A potential candidate for the Anthropocene GSSP by Cale AC Gushulak, Matthew Marshall, Brian F Cumming, Brendan Llew-Williams, R Timothy Patterson and Francine MG McCarthy in The Anthropocene Review
MPEC 2024-L10 : DAILY ORBIT UPDATE (2024 June 1)
Creators: name: Minor Planet Center Staff
Publisher: name: The Minor Planet Center
Year: 2024
Subjects: subject: Small Solar System bodies
valueUri: http://astrothesaurus.org/uat/1469

description: The Minor Planet Electronic Circulars contain information on unusual minor planets, routine data on comets and natural satellites, and occasional editorial announcements.  They are published on behalf of Division F of the International Astronomical Union by the Minor Planet Center, Smithsonian Astrophysical Observatory, Cambridge, MA 02138, U.S.A.
STOCHASTIC MOBILITY MODELS IN SPACE AND TIME
Creators: name: VALERIO, LORENZO
Publisher: name: Universit� degli Studi di Milano
Year: 2012

description: An interesting fact in nature is that if we observe agents (neurons, particles, animals, humans) behaving, or more precisely moving, inside their environment, we can recognize - tough at different space or time scales - very specific patterns. The existence of those patterns is quite obvious, since not all things in nature behave totally at random, especially if we take into account thinking species like human beings. If a first phenomenon which has been deeply modeled is the gas particle motion as the template of a totally random motion, other phenomena, like foraging patterns of animals such as albatrosses, and specific instances of human mobility wear some randomness away in favor of deterministic components. Thus, while the particle motion may be satisfactorily described with a Wiener Process (also called Brownian motion), the others are better described by other kinds of stochastic processes called Levy Flights.
Minding at these phenomena in a unifying way, in terms of motion of agents ? either inanimate like the gas particles, or animated like the albatrosses ? the point is that the latter are driven by specific interests, possibly converging into a common task, to be accomplished. The whole thesis work turns around the concept of agent intentionality at different scales, whose model may be used as key ingredient in the statistical description of complex behaviors. The two main contributions in this direction are:
1. the development of a ?wait and chase? model of human mobility having the same two-phase pattern as animal foraging but with a greater propensity of local stays in place and therefore a less dispersed general behavior;
2. the introduction of a mobility paradigm for the neurons of a multilayer neural network and a methodology to train these new kind of networks to develop a collective behavior. The lead idea is that neurons move toward the most informative mates to better learn how to fulfill their part in the overall
functionality of the network.
With these specific implementations we have pursued the general goal of attributing both a cognitive and a physical meaning to the intentionality so as to be able in a near future to speak of intentionality as an additional potential in the dynamics of the masses (both at the micro and a the macro-scale), and of communication as another network in the force field. This could be intended as a step ahead in the track opened by the past century physicists with the coupling of thermodynamic and Shannon entropies in the direction of unifying cognitive and physical laws.
Mechanical and wear behaviour of boron carbide fillers reinforced Lapox L-12 epoxy composites
Creators: name: Harigovindan, Anitha Devi Sonepalli
name: Hawanje Ghanashyam Shenoy
name: Ravindra
name: Virupaxi Auradi
Publisher: name: Faculty of Engineering, Khon Kaen University
Year: 2024
Subjects: B4C Fillers
Epoxy L-12
Polymer composites
Tensile properties
Flexural strength
Wear

description: Engineering and Applied Science Research, 51, 4, 524-532
Voxel-based point cloud localization for smart spaces management
Creators: name: Mortazavi, F. S.
name: Shkedova, O.
name: Feuerhake, U.
name: Brenner, C.
name: Sester, M.
Publisher: name: Katlenburg-Lindau : Copernicus
Year: 2023
Subjects: subject: Digital twins
subject: ICP
subject: Localization
subject: Parking space management
subject: RANSAC
subject: Voxels
subject: Konferenzschrift
subject: Dewey Decimal Classification::500 | Naturwissenschaften::550 | Geowissenschaften

description: This paper proposes a voxel-based approach for creating a digital twin of an urban environment that is capable of efficiently managing smart spaces. The paper explains the registration and localization procedure of the point cloud dataset, which uses the KISS ICP for scan point cloud combination and the RANSAC method for the initial alignment of the combined point cloud. The mobile mapping point cloud using Riegl VMX-250 serves as the reference map, and Velodyne scans are used for localization purposes. The point-to-plane iterative closest-point method is then employed to refine the alignment. The paper evaluates the efficacy of the proposed method by calculating the errors between the estimated and ground truth positions. The results indicate that the voxel-based approach is capable of accurately estimating the position of the sensor platform, which are applicable for various use cases. A specific use case in the context is smart parking space management, which is described and initial visualization results are shown.
Relationship between structural and mechanical properties of polyethylene matrix nanocomposites
Creators: name: Fath Eddine Zakaria Rahmaoui
name: Idir Belaidi
Publisher: name: Faculty of Engineering, Khon Kaen University
Year: 2024
Subjects: Nanocomposites
Graphene nanoplatelets
Crystallinity
Mechanical properties

description: Engineering and Applied Science Research, 51, 4, 533-539
sj-docx-1-jpe-10.1177_0739456X211044215 – Supplemental material for Changing Trends in Long-Term Sentiments and Neighborhood Determinants in a Shrinking City
Creators: name: Park, Yunmi
name: Kim, Minju
name: Shin, Jiyeon
name: Heim LaFrombois, Megan E.
Publisher: name: SAGE Journals
Year: 2021
Subjects: subject: 120599 Urban and Regional Planning not elsewhere classified
subject: FOS: Social and economic geography

description: Supplemental material, sj-docx-1-jpe-10.1177_0739456X211044215 for Changing Trends in Long-Term Sentiments and Neighborhood Determinants in a Shrinking City by Yunmi Park, Minju Kim, Jiyeon Shin and Megan E. Heim LaFrombois in Journal of Planning Education and Research
Additional file 3 of The efficacy and safety of apatinib plus capecitabine in platinum-refractory metastatic and/or recurrent nasopharyngeal carcinoma: a prospective, phase II trial
Creators: name: Tang, Lin-Quan
name: Li, Xiao-Yun
name: Li, Zhi-Ming
name: Liu, Zhi-Gang
name: Lin, Miao-Zhen
name: Zhou, Huan
name: Yu, Qi-Wen
name: Zhou, Jian
name: Zhao, Chong
name: Chen, Ze-Bin
name: Wang, Xi-Cheng
name: Peng, Jia-Yu
name: Chen, Qiu-Yan
name: Fang, Wen-Feng
name: Yang, Yun-Peng
name: Zhang, Bei
name: Xia, Liang-Ping
name: Hu, Pi-Li
name: Hu, Wei-Han
name: Li, Yi-Jie
name: Mai, Hai-Qiang
name: Cai, Xiu-Yu
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Environmental Sciences not elsewhere classified
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Science Policy
subject: Infectious Diseases
subject: FOS: Health sciences

description: Additional file 3: Table S1. Information on death cases
The novel chloroplast glucose transporter pGlcT2 affects adaptation to extended light periods
Creators: name: Valifard, Marzieh
name: Fernie, Alisdair R.
name: Kitashova, Anastasia
name: Nägele, Thomas
name: Schröder, Rebekka
name: Meinert, Melissa
name: Pommerrenig, Benjamin
name: Mehner-Breitfeld, Denise
name: Witte, Claus-Peter
name: Brüser, Thomas
name: Keller, Isabel
name: Neuhaus, H. Ekkehard
Publisher: name: Bethesda, Md. : ASBMB
Year: 2023
Subjects: subject: Arabidopsis thaliana
subject: chloroplast
subject: glucose transport
subject: membrane protein
subject: photosynthesis
subject: Dewey Decimal Classification::500 | Naturwissenschaften::540 | Chemie
subject: Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie

description: Intracellular sugar compartmentation is critical in plant development and acclimation to challenging environmental conditions. Sugar transport proteins are present in plasma membranes and in membranes of organelles such as vacuoles, the Golgi apparatus, and plastids. However, there may exist other transport proteins with uncharacterized roles in sugar compartmentation. Here we report one such novel transporter of the Monosaccharide Transporter Family, the closest phylogenetic homolog of which is the chloroplast-localized glucose transporter pGlcT and that we therefore term plastidic glucose transporter 2 (pGlcT2). We show, using gene-complemented glucose uptake deficiency of an Escherichia coli ptsG/manXYZ mutant strain and biochemical characterization, that this protein specifically facilitates glucose transport, whereas other sugars do not serve as substrates. In addition, we demonstrate pGlcT2-GFP localized to the chloroplast envelope and that pGlcT2 is mainly produced in seedlings and in the rosette center of mature Arabidopsis plants. Therefore, in conjunction with molecular and metabolic data, we propose pGlcT2 acts as a glucose importer that can limit cytosolic glucose availability in developing pGlcT2-overexpressing seedlings. Finally, we show both overexpression and deletion of pGlcT2 resulted in impaired growth efficiency under long day and continuous light conditions, suggesting pGlcT2 contributes to a release of glucose derived from starch mobilization late in the light phase. Together, these data indicate the facilitator pGlcT2 changes the direction in which it transports glucose during plant development and suggest the activity of pGlcT2 must be controlled spatially and temporarily in order to prevent developmental defects during adaptation to periods of extended light.