title: ÐÐ¾Ð·ÑÐ°ÑÑÐ½ÑÐµ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÑ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð² Ð½ÐµÐ¹ÑÐ¾Ð½Ð¾Ð² CA1 Ð¾Ð±Ð»Ð°ÑÑÐ¸ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð° Ð² Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ Ð¥Ð°Ð½ÑÐ¸Ð½Ð³ÑÐ¾Ð½Ð° in vivo
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Age-related changes in the dendritic spineâs morphology of hippocampal CA1 neurons in the model of Huntington's disease in vivo
Creators: name: ÐÑÐ¸ÑÐ¸Ð½Ð°, ÐÐ»Ð¸Ð·Ð°Ð²ÐµÑÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: Ð±Ð¾Ð»ÐµÐ·Ð½Ñ Ð¥Ð°Ð½ÑÐ¸Ð½Ð³ÑÐ¾Ð½Ð°
Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÐµ ÑÐ¸Ð¿Ð¸ÐºÐ¸
Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿
Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ñ
Ð½ÐµÐ¹ÑÐ¾Ð´ÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸Ð²Ð½ÑÐµ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ñ
Huntington's disease
dendritic spines
hippocampus
morphology
neurodegenerative diseases

description: Ð Ð°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÑ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð² Ð½ÐµÐ¹ÑÐ¾Ð½Ð¾Ð² Ð¡Ð1 Ð¾Ð±Ð»Ð°ÑÑÐ¸ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð° Ð½Ð° Ð¼ÑÑÐ¸Ð½Ð¾Ð¹ Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ Ð¥Ð°Ð½ÑÐ¸Ð½Ð³ÑÐ¾Ð½Ð°. ÐÐ»Ñ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð¸Ñ Ð¿Ð¾ÑÑÐ°Ð²Ð»ÐµÐ½Ð½ÑÑ Ð·Ð°Ð´Ð°Ñ Ð±ÑÐ»Ð¸ Ð¾ÑÐ²Ð¾ÐµÐ½Ñ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð¼ÐµÑÐ¾Ð´Ñ: Ð³ÐµÐ½Ð¾ÑÐ¸Ð¿Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ, ÑÑÐ°Ð½ÑÐºÐ°ÑÐ´Ð¸Ð°Ð»ÑÐ½Ð°Ñ Ð¿ÐµÑÑÑÐ·Ð¸Ñ, Ð¿ÑÐ¸Ð³Ð¾ÑÐ¾Ð²Ð»ÐµÐ½Ð¸Ðµ ÑÐ¸ÐºÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ ÑÑÐµÐ·Ð¾Ð², ÐºÐ¾Ð½ÑÐ¾ÐºÐ°Ð»ÑÐ½Ð°Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ, Ð°Ð½Ð°Ð»Ð¸Ð· Ð¼Ð¸ÐºÑÐ¾ÑÐ¾ÑÐ¾Ð³ÑÐ°ÑÐ¸Ð¹ Ð¸ ÑÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð°Ð½Ð°Ð»Ð¸Ð·. ÐÐ»Ñ Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð¿Ð°ÑÐ¾Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð½ÐµÐ¹ÑÐ¾Ð½Ð°Ð»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº in vivo Ð±ÑÐ»Ð° Ð²ÑÐ²ÐµÐ´ÐµÐ½Ð° Ð½Ð¾Ð²Ð°Ñ ÑÑÐ°Ð½ÑÐ³ÐµÐ½Ð½Ð°Ñ Ð»Ð¸Ð½Ð¸Ñ Ð¼ÑÑÐµÐ¹ YAC128-M. Ð¡ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÐºÐ¾Ð½ÑÐ¾ÐºÐ°Ð»ÑÐ½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸ Ð±ÑÐ»Ð¸ ÑÐ´ÐµÐ»Ð°Ð½Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐ¾ÑÐ¾Ð³ÑÐ°ÑÐ¸Ð¸ ÑÑÐ°Ð³Ð¼ÐµÐ½ÑÐ¾Ð² Ð²ÑÐ¾ÑÐ¸ÑÐ½ÑÑ Ð°Ð¿Ð¸ÐºÐ°Ð»ÑÐ½ÑÑ Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ¾Ð² Ð½ÐµÐ¹ÑÐ¾Ð½Ð¾Ð² Ð¼ÑÑÐµÐ¹ Ð´Ð¸ÐºÐ¾Ð³Ð¾ ÑÐ¸Ð¿Ð° (WT) Ð¸ Ð¼ÑÑÐµÐ¹ ÑÑÐ°Ð½ÑÐ³ÐµÐ½Ð½Ð¾Ð¹ Ð»Ð¸Ð½Ð¸Ð¸ YAC128, Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ ÑÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð¸Ð·Ð¾Ð±ÑÐ°Ð¶ÐµÐ½Ð¸Ð¹ Ð¼Ð¾Ð¶Ð½Ð¾ ÑÐ´ÐµÐ»Ð°ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð²ÑÐ²Ð¾Ð´Ñ. Ð Ð²Ð¾Ð·ÑÐ°ÑÑÐµ 6-ÑÐ¸ Ð¼ÐµÑÑÑÐµÐ² Ñ Ð¼ÑÑÐµÐ¹ YAC128 Ð¸ WT Ð½Ð°Ð±Ð»ÑÐ´Ð°ÑÑÑÑ Ð²ÑÑÐ°Ð¶ÐµÐ½Ð½ÑÐµ Ð·Ð½Ð°ÑÐ¸Ð¼ÑÐµ ÑÐ°Ð·Ð»Ð¸ÑÐ¸Ñ ÐºÐ°Ðº Ð² Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÐ¸ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð², ÑÐ°Ðº Ð¸ Ð² ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ðµ Ð³ÑÐ¸Ð±Ð¾Ð²Ð¸Ð´Ð½ÑÑ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð² Ð² ÑÐ°ÑÑÐ½Ð¾ÑÑÐ¸, Ð¿ÑÐ¸ÑÐµÐ¼ Ð¿Ð¾ÐºÐ°Ð·Ð°ÑÐµÐ»Ð¸ ÑÑÐ°Ð½ÑÐ³ÐµÐ½Ð½ÑÑ Ð¼ÑÑÐµÐ¹ Ð¿ÑÐµÐ²Ð¾ÑÑÐ¾Ð´ÑÑ Ð·Ð½Ð°ÑÐµÐ½Ð¸Ñ Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÐ¸ Ð¸ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð° ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð² Ð´Ð»Ñ Ð´Ð¸ÐºÐ¸Ñ Ð¼ÑÑÐµÐ¹. Ð Ð²Ð¾Ð·ÑÐ°ÑÑÐµ 9-ÑÐ¸ Ð¼ÐµÑÑÑÐµÐ² Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÑ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð² Ð½ÐµÐ¹ÑÐ¾Ð½Ð¾Ð² WT Ð·Ð½Ð°ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾ ÑÐ²ÐµÐ»Ð¸ÑÐ¸Ð²Ð°ÐµÑÑÑ, Ð² Ð½ÐµÐ¹ÑÐ¾Ð½Ð°Ñ ÑÑÐ°Ð½ÑÐ³ÐµÐ½Ð½ÑÑ Ð¼ÑÑÐµÐ¹ Ð¿Ð¾Ð´Ð¾Ð±Ð½ÑÑ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹ Ð½Ðµ Ð¿ÑÐ¾Ð¸ÑÑÐ¾Ð´Ð¸Ñ, ÐºÑÐ¾Ð¼Ðµ ÑÐ¾Ð³Ð¾ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð¾ Ð³ÑÐ¸Ð±Ð¾Ð²Ð¸Ð´Ð½ÑÑ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð² Ð·Ð°Ð¼ÐµÑÐ½Ð¾ ÑÐ½Ð¸Ð¶Ð°ÐµÑÑÑ Ð¿Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ Ñ 6-Ñ Ð¼ÐµÑÑÑÐ°Ð¼Ð¸. Ð ÑÐ°Ð¼ÐºÐ°Ñ Ð³ÑÑÐ¿Ð¿Ñ 9-ÑÐ¸ Ð¼ÐµÑÑÑÐµÐ² ÑÐ°Ð·Ð½Ð¸ÑÐ° Ð² ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ðµ Ð³ÑÐ¸Ð±Ð¾Ð²Ð¸Ð´Ð½ÑÑ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð² Ð½Ðµ ÑÐ²Ð»ÑÐµÑÑÑ ÑÑÑÐµÑÑÐ²ÐµÐ½Ð½Ð¾Ð¹, Ð¾Ð´Ð½Ð°ÐºÐ¾ ÐµÑÐ»Ð¸ ÑÐ°ÑÑÐ¼Ð°ÑÑÐ¸Ð²Ð°ÑÑ Ð²Ð¾Ð·ÑÐ°ÑÑÐ½ÑÑ Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÑ Ð½Ð° ÑÑÐ°Ð½ÑÐ³ÐµÐ½Ð½Ð¾Ð¼ Ð³ÐµÐ½Ð¾ÑÐ¸Ð¿Ðµ, ÑÐ¾ Ð¾Ð½Ð° Ð¸Ð¼ÐµÐµÑ Ð±Ð¾Ð»ÑÑÐ¾Ðµ Ð·Ð½Ð°ÑÐµÐ½Ð¸Ðµ.
description: The given work is devoted to the study of the dendritic spineâs morphology of the hippocampal CA1 neurons in a mouse model of Huntington's disease. To perform the tasks, the following methods were mastered: genotyping, transcardial perfusion, preparation of fixed brain sections, confocal microscopy, analysis of micrographs and statistical analysis. A new transgenic mouse line YAC128-M was developed for the study of neuronal cells pathomorphology in vivo. Microphotographs of secondary apical dendrites fragments of neurons of wild-type (WT) mice and YAC128 transgenic mice were made using confocal microscopy, and the following conclusions can be drawn based on statistical analysis of the images. At the age of 6 months, YAC128 and WT mice showed pronounced significant differences in both the density of spines and the number of mushroom spines, and the indicators of transgenic mice exceeded the values of the density and number of spines for wild mice. At the age of 9 months, the density of WT neuronâs spines increases significantly, in the neurons of transgenic mice such changes do not occur, in addition, the number of mushroom-shaped spines significantly decreases compared to 6 months. Within the group of 9 months, the difference in the number of mushroom spines is not significant, but if we consider the age dynamics on the transgenic genotype, it is of great importance.