Polymorphisms in xenobiotic metabolism-related genes in patients with hepatocellular carcinoma: a case–control study
Creators: name: Ferreira, Gislaine Dionísio
name: Glaucia Maria De Mendonça Fernandes
name: Penteado, Camila
name: Cória, Vivian Romanholi
name: Da Silva Galbiatti-Dias, Ana Lívia
name: Russo, Anelise
name: Castanhole-Nunes, Márcia Maria Urbanin
name: Da Silva, Renato Ferreira
name: De Cássia Martins Alves Da Silva, Rita
name: Érika Cristina Pavarino
name: Cintra, Mariangela Torreglosa Ruiz
name: Eny Maria Goloni-Bertollo
Publisher: name: Taylor & Francis
Year: 2021
Subjects: subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: FOS: Earth and related environmental sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Cancer

description: This study was performed to investigate the relationship between polymorphisms in microsomal epoxide hydrolase (<i>mEH</i>; Tyr113His and His139Arg substitution) and glutathione S-transferase (<i>GST</i>; <i>GSTM1</i> deletion, <i>GSTT1</i> deletion, and <i>GSTP1</i>.Ala114Val substitution) and their correlation with clinico-histopathological features in hepatocellular carcinoma (HCC).We evaluated environmental risk factors and genetic alterations in 556 individuals (86 cases and 470 controls). PCR multiplex for <i>GSTM1</i> and <i>GSTT1</i>, polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) for <i>GSTP1</i>, and real-time PCR for <i>mEH</i> were performed. Statistical analyses were performed using multiple logistic regression tests.Age over 48 years (<i>p</i> p = 0.021) were the predictors of increased risk of developing HCC. <i>GSTP1</i>.Ala114Val for all regression models (<i>p</i> GSTT1 null genotype (odds ratio [OR] = 0.43, 95% confidence interval [CI] = 0.21–0.87, <i>p</i> = 0.019) were predictors of an increased risk of developing HCC. Polymorphic <i>GSTT1, GSTM1</i>, <i>GSTP1.</i>Ala114Val, and <i>mEH</i>.His139Arg and wild-type mEH.Tyr113His (OR = 5.04; 95% CI = 1.59–16.04; <i>p</i> = 0.006) were associated with HCC.Age over 48 years, alcohol consumption, and the presence of polymorphic variants of <i>GSTP1</i> and <i>GSTT1</i> were associated with the risk of developing HCC. This study was performed to investigate the relationship between polymorphisms in microsomal epoxide hydrolase (<i>mEH</i>; Tyr113His and His139Arg substitution) and glutathione S-transferase (<i>GST</i>; <i>GSTM1</i> deletion, <i>GSTT1</i> deletion, and <i>GSTP1</i>.Ala114Val substitution) and their correlation with clinico-histopathological features in hepatocellular carcinoma (HCC). We evaluated environmental risk factors and genetic alterations in 556 individuals (86 cases and 470 controls). PCR multiplex for <i>GSTM1</i> and <i>GSTT1</i>, polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) for <i>GSTP1</i>, and real-time PCR for <i>mEH</i> were performed. Statistical analyses were performed using multiple logistic regression tests. Age over 48 years (<i>p</i> p = 0.021) were the predictors of increased risk of developing HCC. <i>GSTP1</i>.Ala114Val for all regression models (<i>p</i> GSTT1 null genotype (odds ratio [OR] = 0.43, 95% confidence interval [CI] = 0.21–0.87, <i>p</i> = 0.019) were predictors of an increased risk of developing HCC. Polymorphic <i>GSTT1, GSTM1</i>, <i>GSTP1.</i>Ala114Val, and <i>mEH</i>.His139Arg and wild-type mEH.Tyr113His (OR = 5.04; 95% CI = 1.59–16.04; <i>p</i> = 0.006) were associated with HCC. Age over 48 years, alcohol consumption, and the presence of polymorphic variants of <i>GSTP1</i> and <i>GSTT1</i> were associated with the risk of developing HCC.