cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Differentiation of Different Nonmelanoma Skin Cancer Types Using OCT
Creators: name: Batz, Sebastian
name: Wahrlich, Caroline
name: Alawi, Arash
name: Ulrich, Martina
name: Lademann, Jürgen
Publisher: name: Charité - Universitätsmedizin Berlin
Year: 2018
Subjects: subject: Actinic keratosis
subject: Basal cell carcinoma
subject: Basalioma
subject: Superficial basal cell carcinoma
subject: Morbus Bowen
subject: Squamous cell carcinoma
subject: Optical coherence tomography
subject: 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit

description: Background: Early detection of various types of nonmelanoma skin cancer has been a challenge in dermatology. Noninvasive examination procedures such as optical coherence tomography (OCT) play an increasingly important role, besides the established gold standard of histological tissue sample analysis. OCT is a noninvasive, cross-sectional, real-time technique that allows conclusions to be drawn with regard to the presence of pathologies. Objective: The objective of this study was to investigate whether it is possible to distinguish between different types of nonmelanoma skin cancer using OCT or not. Methods: A study population of a total of 25 cases, comprising 5 cases, each, of 5 tumor entities (i.e., basal cell carcinoma, superficial basal cell carcinoma, actinic keratosis, squamous cell carcinoma, and Bowen disease) was examined. Relevant lesions were scanned both centrally and peripherally in the multislice mode. All OCT images were blinded, randomized, analyzed, and evaluated by 2 clinicians experienced in OCT. Results: This study demonstrated that it is possible to determine correlations between various types of tumors and recurring tumor characteristics. Conclusion: This study showed that it is possible to distinguish between the different nonmelanoma skin cancers by using OCT, but further prospective studies have to be conducted to validate the sensitivity and specificity of the criteria.
Long-Term Follow-Up after Paediatric Kidney Transplantation and Influence Factors on Graft Survival: A Single-Centre Experience of 16 years
Creators: name: Friedersdorff, Frank
name: Koch, Therese-Marie
name: Banuelos-Marco, Beatriz
name: Gonzalez, Ricardo
name: Fuller, Tom Florian
name: Mechow, Stefanie Von
name: Müller, Dominik
name: Lingnau, Anja
Publisher: name: Charité - Universitätsmedizin Berlin
Year: 2018
Subjects: subject: Influence factors
subject: Paediatric transplantation
subject: Vascular complications
subject: 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit

description: Introduction: To evaluate graft- and patient survival after paediatric kidney transplantation and detecting influence factors, which affect the post-transplant time. Materials and Methods: We analysed long-term survival rates and complications after paediatric kidney transplantation and searched for predictive parameters for graft function. Results: In 132 patients, 143 kidney transplantations were performed. Graft failure occurred in 25%. Chronic rejections were the leading cause of graft loss (42.9%). Graft survival rates were 92.2% after 1 year, 85.5% after 5 years, 71.1% after 10 years and 62.1% after 15 years. The following parameters strongly influenced graft survival: number of transplants (p = 0.014), year of transplant (p < 0.0001 for 1997-2005), Epo-therapy post-transplant (p = 0.001), hypotension donor (p = 0.027), cold ischemia time (p = 0.023), anastomosis time >50 min (p = 0.008), delayed graft function (p = 0.003) and deceased donation (p = 0.039). The percentage of patients who died was 5.6%. Overall patient survival rates were 99.3% after 1 year, 95.2% after 5 years, 94.2% after 10 years and 90.7% after 15 years. Various types of infections (42.9%) were the main causes of death. Conclusions: The main causes of death after kidney transplantations in paediatric recipients are malignancy and infections. To avoid vascular complications especially in young recipients (< 9 years), the cold ischemia time should be as short as possible.
Effect of Fluid Intake on Hydration Status and Skin Barrier Characteristics in Geriatric Patients: An Explorative Study
Creators: name: Akdeniz, Merve
name: Boeing, Heiner
name: Müller-Werdan, Ursula
name: Aykac, Volkan
name: Steffen, Annika
name: Schell, Mareike
name: Blume-Peytavi, Ulrike
name: Kottner, Jan
Publisher: name: Charité - Universitätsmedizin Berlin
Year: 2018
Subjects: subject: Transepidermal water loss
subject: pH
subject: Dehydration
subject: Skin physiology
subject: Drinking
subject: Geriatrics
subject: 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit

description: Background/Aim: Inadequate fluid intake is assumed to be a trigger of water-loss dehydration, which is a major health risk in aged and geriatric populations. Thus, there is a need to search for easy to use diagnostic tests to identify dehydration. Our overall aim was to investigate whether skin barrier parameters could be used for predicting fluid intake and/or hydration status in geriatric patients. Methods: An explorative observational comparative study was conducted in a geriatric hospital including patients aged 65 years and older. We measured 3-day fluid intake, skin barrier parameters, Overall Dry Skin Score, serum osmolality, cognitive and functional health, and medications. Results: Forty patients were included (mean age 78.45 years and 65% women) with a mean fluid intake of 1,747 mL/day. 20% of the patients were dehydrated and 22.5% had an impending dehydration according to serum osmolality. Multivariate analysis suggested that skin surface pH and epidermal hydration at the face were associated with fluid intake. Serum osmolality was associated with epidermal hydration at the leg and skin surface pH at the face. Fluid intake was not correlated with serum osmolality. Diuretics were associated with high serum osmolality. Conclusions: Approximately half of the patients were diagnosed as being dehydrated according to osmolality, which is the current reference standard. However, there was no association with fluid intake, questioning the clinical relevance of this measure. Results indicate that single skin barrier parameters are poor markers for fluid intake or osmolality. Epidermal hydration might play a role but most probably in combination with other tests.
Additional file 2 of Psychosocial barriers and facilitators for adherence to a healthy lifestyle among patients with chronic kidney disease: a focus group study
Creators: name: Cardol, Cinderella K.
name: Boslooper-Meulenbelt, Karin
name: van Middendorp, Henriët
name: Meuleman, Yvette
name: Evers, Andrea W. M.
name: van Dijk, Sandra
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Sociology
subject: FOS: Sociology
subject: Marine Biology
subject: Cancer
subject: 111714 Mental Health
subject: FOS: Health sciences

description: Additional file 2.
Additional file 1 of 2-deoxy-d-glucose as an adjunct to standard of care in the medical management of COVID-19: a proof-of-concept and dose-ranging randomised phase II clinical trial
Creators: name: Bhatt, Anant Narayan
name: Shenoy, Srinivas
name: Munjal, Sagar
name: Chinnadurai, Vijayakumar
name: Agarwal, Apurva
name: Vinoth Kumar, A.
name: Shanavas, A.
name: Kanwar, Ratnesh
name: Chandna, Sudhir
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Pharmacology
subject: Biotechnology
subject: Cancer
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: 60506 Virology
subject: FOS: Biological sciences

description: Additional file 1: Fig. S1 Chemical structures of glucose (left) and 2-deoxy-D-glucose (2-DG) (right). Fig. S2 Probability of achieving and maintaining blood oxygen saturation of ≥94% in patients treated with 2-DG 90 mg/kg/day + SOC compared with the contemporaneous SoC group (SOC2). Table S1 Patient Demographics. Table S2 Medical History. Table S3 Summary of Time to Clinical Recovery. Table S4 Analysis of Time to Clinical Recovery. Table S5 Proportion of Patients Showing Negative Conversion of Detectable SARS-CoV-2 RNA on RT-PCR by Study Days 3, 7, 10, and EOT. Table S6 Summary of Time to First Negative Conversion of Detectable SARS-CoV-2 By RT-PCR Assay of a Nasopharyngeal/Oropharyngeal Swab Specimen. Table S7 Analysis of Time to First Negative Conversion of Detectable SARS-CoV-2 by RT-PCR Assay of a Nasopharyngeal/Oropharyngeal Swab Specimen. Table S8 Summary of Time to Discharge from Isolation Ward. Table S9 Analysis of Time to Discharge from Isolation Ward. Table S10 Summary of Time to Achieve Improvement by At Least 1 and At Least 2 Points Over Baseline in the Clinical Status Score on WHO 10-Point Ordinal Scale from Start of Study Treatment. Table S11 Analysis of Median Time (no. of days) to Achieve Improvement by 1 Point and by 2 Points Over Baseline in the Clinical Status Score on WHO 10-Point Ordinal Scale from Start of Study Treatment - Study part A. Table S12 Analysis of Median Time (no. of days) to Achieve Improvement by 1 Point and by 2 Points Over Baseline in the Clinical Status Score on WHO 10-Point Ordinal Scale from Start of Study Treatment - Study part B. Table S13 Summary of Patients Requiring Management in Intensive Care Unit (ICU), Oxygen Supplementation, Non-Invasive and Invasive Mechanical Ventilation until Day 28. Table S14 Summary of Time to Achieve and Maintain Blood Oxygen Saturation of ≥94%. Table S15 Analysis of Time to Achieve and Maintain Blood Oxygen Saturation of ≥94%. Table S16 Summary of Time to Vital Signs Normalization. Table S17 Analysis of Time to Vital Signs Normalization. Table S18 Efficacy endpoint comparisons between active (2-DG) and Pooled SOC groups. Table S19 Number and Percentage of patients with TEAEs classified by SOC and PT.
Additional file 3 of Psychosocial barriers and facilitators for adherence to a healthy lifestyle among patients with chronic kidney disease: a focus group study
Creators: name: Cardol, Cinderella K.
name: Boslooper-Meulenbelt, Karin
name: van Middendorp, Henriët
name: Meuleman, Yvette
name: Evers, Andrea W. M.
name: van Dijk, Sandra
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Sociology
subject: FOS: Sociology
subject: Marine Biology
subject: Cancer
subject: 111714 Mental Health
subject: FOS: Health sciences

description: Additional file 3.
Biophysical regulations in neural development
Creators: name: Hsu, Chia-Chen
Publisher: name: Imperial College London
Year: 2018

description: During neural development, in synergy with widely studied biochemical signals, biophysical cues cooperate in the regulation of cell behaviour. In addition to gene and protein expression, recent studies have highlighted the significance of epigenetic modulation on cell fate. Although it is known that extracellular biophysical cues can affect important cellular events, only a few studies have systematically examined their effects on stage-specific developmental patterns and neuronal maturation, particularly in humans. Moreover, very few of them have further linked biophysical modulations to epigenetic mechanisms. This project aims to create a new bioengineered platform incorporating conductive materials with topographical cues, specifically microgrooves, to examine the combinatorial effects of surface topography and electrical stimulation on clinically relevant neuronal populations derived from human pluripotent stem cells (hPSCs). The microgrooved platform and its synergistic effects with electrical stimulation can promote neuronal differentiation. For the first time, an increase in epigenetic markers associated with stemness and differentiation, such as AcH3, AcH4, and H3K9me3, was reported on the microgrooves and after electrical stimulation. Using state-of-the-art focused ion beam scanning electron microscopy, the observed epigenetic changes and corresponding nuclear morphology were examined in high resolution. Moreover, in parallel with topography-dependent epigenetic modulations, a new mechanism was proposed –namely that microgrooves can modulate Notch signalling through geometric segregation. Finally, a new tissue-engineered scaffold based on electrospun serum albumin (SA) was developed with the incorporation of both biochemical and biophysical stimuli. This served as a functional growth factor release construct and positively influenced neuronal differentiation and maturation with electrical stimulation. The thesis presents a systematic examination of cellular behaviour in response to biophysical stimuli, including surface topography and electrical stimuli. Together, novel mechanisms associated with epigenetics and signalling pathways as well as the new SA based scaffold, can advance fundamental research in neuroscience and future translational applications.
Additional file 4 of Psychosocial barriers and facilitators for adherence to a healthy lifestyle among patients with chronic kidney disease: a focus group study
Creators: name: Cardol, Cinderella K.
name: Boslooper-Meulenbelt, Karin
name: van Middendorp, Henriët
name: Meuleman, Yvette
name: Evers, Andrea W. M.
name: van Dijk, Sandra
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Sociology
subject: FOS: Sociology
subject: Marine Biology
subject: Cancer
subject: 111714 Mental Health
subject: FOS: Health sciences

description: Additional file 4.
New strategies for detailed visualisation and analysis of stem cell-material interactions
Creators: name: Gopal, Sahana
Publisher: name: Imperial College London
Year: 2018

description: The success of stem cell therapy and regenerative medicine relies on providing bio- chemical and biophysical cues to safely and reliably control cell fate transitions. It has now been recognised that recapitulating the physical attributes of the stem cell microenvironment is a key feature in controlling cell behaviour. Of particular interest is the ability of the extracellular microenvironment to present surface topographical features that contribute to control of cell attachment and morphology, which have a direct impact on maintenance of cell phenotype and gene expression. This realisation has led to the development of materials with surface topography to probe and control the stem cell behaviour. In this thesis, the influence of material topography on cell behaviour is investigated through the use of both microscale and nanoscale topography, with a particular focus on visualisation strategies to characterise the features of the cell-material interface. Firstly, microscale topography in the form of microgrooves, is used to study the impact of topography at the level of the nucleus in neural stem cells. Through the development of new visualisation methods, incorporating state-of-the-art focused-ion beam scanning electron microscopy and scanning ion conductance microscopy, the nuclei of cells cul- tured on microgrooved topography are shown to correlatively remodel their nuclear architecture, epigenetics and mechanical properties. Secondly, using nanoscale topography in the form of high aspect ratio nanoneedle arrays, the cell-material interface is investigated at the level of the plasma membrane in human mesenchymal stem cells. Nanoneedles, typically used to deliver a variety of therapeutically relevant payloads, are shown to create a tight interface with the cell membrane, while triggering changes in plasma membrane architecture and inducing the activation of endocytosis pathways, which contributes significantly to payload delivery at the interface.
Additional file 1 of Study protocol for a pragmatic cluster randomized controlled trial to improve dietary diversity and physical fitness among older people who live at home (the “ALAPAGE study”)
Creators: name: Bocquier, Aurélie
name: Jacquemot, Anne-Fleur
name: Dubois, Christophe
name: Tréhard, Hélène
name: Cogordan, Chloé
name: Maradan, Gwenaëlle
name: Cortaredona, Sébastien
name: Fressard, Lisa
name: Davin-Casalena, Bérengère
name: Vinet, Agnès
name: Verger, Pierre
name: Darmon, Nicole
name: Arquier, Valérie
name: Briclot, Guillaume
name: Chamla, Rachel
name: Cousson-Gélie, Florence
name: Danthony, Sarah
name: Delrieu, Karin
name: Dessirier, Julie
name: Féart, Catherine
name: Fusinati, Christine
name: Gazan, Rozenn
name: Gibert, Mélissa
name: Lamiraud, Valérie
name: Maillot, Matthieu
name: Nadal, Dolorès
name: Trotta, Christelle
name: Verger, Eric O.
name: Viriot, Valérie
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Physiology
subject: FOS: Biological sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Science Policy
subject: 111714 Mental Health
subject: FOS: Health sciences

description: Additional file 1. SPIRIT 2013 Checklist.
Three wishes: a projective tool in understanding the effects of adverse childhood experiences
Creators: name: O'Donnell, Caitlin
Publisher: name: No Publisher Supplied
Year: 2020

description: Asking for “three wishes” is a projective tool commonly used by clinicians with children to assess features of their internal world. Despite its widespread use, research on the meaning of individuals’ responses to this tool is limited. Some research has looked at different types of wish responses across demographics such as age and gender; however, no study to date has specifically investigated the relationship of wishes to adverse childhood experiences. Adverse childhood experiences (ACEs), such as neglect and abuse, have been specifically connected to later health problems such as obesity and depression (Felitti et al., 1998). To fill in the gap in current literature, this research conducted two studies using an exploratory sequential mixed-methods approach. One examined the wishes of a foster care population and the other examined wishes in a general adult population across a range of early adverse experiences. Our findings are the beginning of understanding common and differing themes between wishes of children and adults. We explore previously unidentified differences such as the desire for reunification and escape in foster children and the centricity of mortality and money in a general adult population. Additionally, we found one theme common across all populations we researched, the wish for attachment and belonging. We also looked at wishes to examine differences across development and early adverse experiences. Across development, we found wish patterns similar to some of the findings of Erik Erikson (Erikson, 1982). Younger children were associated with more material wishes, latency-age children with more social wishes (attachment and belonging), and adolescents with more future-oriented wishes (achievement). Adults higher in adverse childhood experiences had greater difficulty generating wishes, wanted escape more, reported fewer wishes for money, and reported more negative feelings after remembering wishes from childhood. We hope this research will help future clinicians pause to contemplate the meaning behind answers to Three Wishes and/or other interventions, to reflect on the evoked feelings, and to notice individuals’ resilience and aspirations in the face of adversity. We hope this study helps clinicians see what an individual’s wish has in common with wishes of others as well as its unique importance for the individual and their treatment.
Additional file 1 of Residential secondhand smoke in a densely populated urban setting: a qualitative exploration of psychosocial impacts, views and experiences
Creators: name: Tan, Grace Ping Ping
name: Teo, Odelia
name: van der Eijk, Yvette
Publisher: name: figshare
Year: 2022
Subjects: subject: Sociology
subject: FOS: Sociology

description: Additional file 1. Codebook with categories (in bold), themes, sub-themes, number of interviewees endorsing each theme, and sample quotations. KI Key informant, NS Non-smoker, S Smoker, SHS Secondhand smoke.
Metabolomics in bone tissue engineering: a sensitive and robust tool for the evaluation of osteogenic differentiation in 2D and 3D cultures
Creators: name: Klontzas, Michail
Publisher: name: Imperial College London
Year: 2018

description: The number of surgical procedures requiring bone grafts is constantly increasing every year. Current grafting strategies have drawbacks such as donor site morbidity, limited availability, high costs, limited efficiency and the transmission of pathogens. Tissue engineering can offer an alternative to current treatments with the production of engineered bone grafts combining biomaterials, stem cells and osteoinductive signals. To date, no single technique exists, that can independently evaluate the quality of mesenchymal stem cell (MSC) osteogenic differentiation in a specific and sensitive manner. Instead, low sensitivity, low specificity and expensive techniques have to be combined often with conflicting results. Evidence shows that MSC metabolism changes during osteogenic differentiation and such changes can be comprehensively examined with the use of metabolomics. Therefore, given the fact that metabolism dynamically receives and responds to both genetic and environmental signals and reflects the cellular phenotype, the application of metabolomics has been assessed as a robust and sensitive technique to evaluate osteogenic differentiaton. This thesis has utilised umbilical cord derived MSCs which are a promising stem cell source for tissue engineering purposes. Metabolomics have been used to study the physiology of undifferentiated MSCs and comprehensively examine MSC metabolic physiology during 2D osteogenic differentiation with two different osteoinductive agents. The results presented herein showed that metabolism of cells differentiated with the most potent agent, closely resembled metabolism of primary osteoblasts. Subsequently, metabolomics was sensitive enough to successfully evaluate the efficiency of novel RGD-functionalised 3D scaffolds to induce osteogenic differentiation. Finally, novel alginate hydrogels have been developed with the glycyl-L-histidyl-L-lysine peptide (GHK) peptide, showing that metabolomics can provide important information during biomaterial development, by indicating the efficiency of GHK hydrogels to promote osteogenesis, assessing the reproducibility of cellular phenotype and assisting in the delineation of mechanisms underlying the function of GHK.
title: Generalized Cost-Based Job Scheduling in Very Large Heterogeneous Cluster Systems
Creators: name: Khuda Bukhsh, Wasiur R.
name: Kar, Sounak
name: Alt, Bastian
name: Rizk, Amr
name: Koeppl, Heinz
Publisher: name: IEEE
Year: 2022

description: We study job assignment in large, heterogeneous resource-sharing clusters of servers with finite buffers. This load balancing problem arises naturally in today's communication and big data systems, such as Amazon Web Services, Network Service Function Chains, and Stream Processing. Arriving jobs are dispatched to a server, following a load balancing policy that optimizes a performance criterion such as job completion time. Our contribution is a randomized Cost-Based Scheduling (CBS) policy in which the job assignment is driven by general cost functions of the server queue lengths. Beyond existing schemes, such as the Join the Shortest Queue (JSQ), the power of d or the SQ(d) and the capacity-weighted JSQ, the notion of CBS yields new application-specific policies such as hybrid locally uniform JSQ. As today's data center clusters have thousands of servers, exact analysis of CBS policies is tedious. In this article, we derive a scaling limit when the number of servers grows large, facilitating a comparison of various CBS policies with respect to their transient as well as steady state behavior. A byproduct of our derivations is the relationship between the queue filling proportions and the server buffer sizes, which cannot be obtained from infinite buffer models. Finally, we provide extensive numerical evaluations and discuss several applications including multi-stage systems.
Integrating theory and experiments to study complex protein dynamics at the surface of lipid membranes
Creators: name: Sanz Hernandez, Maximo
Publisher: name: Imperial College London
Year: 2018

description: The cellular membrane is a complex biophysical environment that occupies a central position in many fundamental biological processes. In association with the membrane there are a wide variety of proteins, macromolecular actors with crucial roles in driving those processes. Being a primary point of contact between the inside and outside of cells, membrane proteins are targeted by the majority of approved therapeutic drugs. New methods that can unveil the mechanisms by which these molecules function are therefore crucial in deepening our biomolecular understanding and can provide rational approaches for the design of novel therapeutic approaches. Conformational dynamics are an important factor in driving the behaviour of membrane proteins. Receptors, channels and other membrane-embedded proteins function by populating distinct structural states and interconverting between them. However, the molecular detail of these heterogeneous processes is extremely challenging to elucidate with the current analytical tools, generally tailored for homogeneous protein states. This thesis focuses on the methodological development of approaches to accurately characterise the dynamical behaviour of membrane proteins. The methods outlined combine the use of experiments and theoretical sampling to generate ensemble descriptions of membrane proteins. Specifically, the experimentally-restrained molecular dynamics approach is further developed for the incorporation of solid-state NMR observables. The accuracy of this method is validated in the context of membrane proteins and subsequently applied to the study of three different biological systems: the cardiac regulatory protein phospholamban, the influenza proton channel M2 and the G-protein coupled receptor CXCR1. The generated ensembles reveal some important conformational transitions in these membrane proteins, and highlight the importance of combining theory and experiments in order to achieve an accurate, detailed description of biomolecular dynamics in lipid membranes.
Additional file 1 of Two approaches to account for genotype-by-environment interactions for production traits and age at first calving in South African Holstein cattle
Creators: name: Ducrocq, Vincent
name: Cadet, Astrid
name: Patry, Clotilde
name: van der Westhuizen, Lene
name: van Wyk, Jacob B.
name: Neser, Frederick Wilhelm Cornelius
Publisher: name: figshare
Year: 2022
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Ecology
subject: Plant Biology

description: Additional file 1: Table S1. Estimates of heritability (in bold) and genetic correlations (below the diagonal) for each region depending the model used (± SE) for Fat yield and Protein yield. Table S2. Contribution of the second eigenvalue of the genetic covariance matrix, correlation between the two genetic effects (± SE) and goodness-of-fit (BIC = 0 for the model without climatic variable) for reaction norm models including a constant and a regression on one climatic variable at a time, in the case of fat yield and protein yield; Lower values of BIC indicate a better fit.
Additional file 1 of Solubilized chlorin e6-layered double hydroxide complex for anticancer photodynamic therapy
Creators: name: Jo, Young-um
name: Sim, HyunJune
name: Lee, Chung-Sung
name: Kim, Kyoung Sub
name: Na, Kun
Publisher: name: figshare
Year: 2022
Subjects: subject: Biochemistry
subject: Space Science
subject: Pharmacology
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Cancer

description: Additional file 1: Fig. S1. (a) ζ-potential of dispersed Ce6, LDH/Ce6, and MLH/Ce6. (b) hydrodynamic size and PDI of MLH/Ce6 with incubation at R.T. (n=3, 1~5 days). Fig. S2. Cellular uptake of MLH/Ce6 against CT-26 cells depending on the incubation time using flow cytometry (concentration of Ce6 = 1 µg/mL). Fig. S3. Cell viability test of MLHs against CT-26 cells at various concentration (incubation time = 24 h).
Additional file 2 of Study protocol for a pragmatic cluster randomized controlled trial to improve dietary diversity and physical fitness among older people who live at home (the “ALAPAGE study”)
Creators: name: Bocquier, Aurélie
name: Jacquemot, Anne-Fleur
name: Dubois, Christophe
name: Tréhard, Hélène
name: Cogordan, Chloé
name: Maradan, Gwenaëlle
name: Cortaredona, Sébastien
name: Fressard, Lisa
name: Davin-Casalena, Bérengère
name: Vinet, Agnès
name: Verger, Pierre
name: Darmon, Nicole
name: Arquier, Valérie
name: Briclot, Guillaume
name: Chamla, Rachel
name: Cousson-Gélie, Florence
name: Danthony, Sarah
name: Delrieu, Karin
name: Dessirier, Julie
name: Féart, Catherine
name: Fusinati, Christine
name: Gazan, Rozenn
name: Gibert, Mélissa
name: Lamiraud, Valérie
name: Maillot, Matthieu
name: Nadal, Dolorès
name: Trotta, Christelle
name: Verger, Eric O.
name: Viriot, Valérie
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Physiology
subject: FOS: Biological sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Science Policy
subject: 111714 Mental Health
subject: FOS: Health sciences

description: Additional file 2. Control group measurement visits’ content.
Pseudomonas aeruginosa type six secretion system (T6SS): The Tse7 DNase effector and post-translational regulation of the H1-T6SS
Creators: name: Pissaridou, Panayiota
Publisher: name: Imperial College London
Year: 2018

description: Pseudomonas aeruginosa is a versatile opportunistic bacterial pathogen. Its ability to successfully colonize various environments and hosts is partly due to the capability of P. aeruginosa to outcompete other bacteria. The Type Six Secretion System (T6SS) is one key weapon giving a competitive advantage. It is a supramolecular contractile machine capable of delivering a plethora of potent antibacterial toxins. P. aeruginosa possesses three T6SSs (H1- to H3-T6SS) and several T6SS-related islands scattered throughout the genome. Some of these islands encode the T6SS puncturing device, VgrG, and additional T6SS-related function such as Toxin-Immunity pairs. The vgrG1b operon is present downstream of the H1-T6SS cluster and is composed of seven genes. This operon is highly conserved in P. aeruginosa genomes except for the toxin-immunity gene pair, tse7-tsi7. This study demonstrates the Cterminal DNase activity of Tse7. Moreover, the study shows that delivery of Tse7 requires the H1-T6SS and the N-terminus of Tse7 PAAR domain tops the VgrG1b spike. Tsi7, protects producing cells from the DNase activity through direct interactions with Tse7. Additional putative nucleases were identified (TpnA and TpnB). These nucleases are encoded in genetic clusters also encoding PAAR proteins. The tpnB cluster encodes a novel T6SS-chaperone type, TapN, which harbours a DUF4123 domain. TapN is required for the association of the toxin TpnB to the PAAR (PA3904). Another protein, PA3906, encoded within the tpnB cluster is also proposed to have a putative chaperone function by interacting with the PAAR tip. 4 Finally, this study investigates the post-translational regulation of the H1-T6SS activity via the repressor TagF. Two mechanisms of TagF action have been proposed. Firstly, TagF directly counteracts Fha, a T6SS activator. Secondly, TagF directly interacts with T6SS components making the so-called baseplate subcomplex, suggesting a new mode of inhibition by blocking T6SS conformational changes and subsequent sheath contraction.
ILK O'RTA ASRLARDA SUD'NING YUKSALISHI.
Creators: name: Xolmatov, Qudratillo
Publisher: name: Zenodo
Year: 2024

description: Ushbu maqolada III-VIII asrlarda Sug‘ning taraqqiy etishi haqida so‘z boradi.Ilk o‘rta asrlarda Buyuk ipak yo‘li yoqasida joylashgan Sug‘d har tomonlama rivojlandi.Ayniqsa xalqaro savdoda sug‘d savdogarlari vositachilikni o‘z qollariga oldilar,sug‘d puli xalqaro pul sifatida,sug‘d tili esa xalqaro til sifatida foydalanildi.Sug‘d savdogarlari va aristokratiya vakillari qo‘shni mamlakatlarda o‘z qarorgohlariga ega bo‘ldilar.Arablar bosqini natijasida rivojlangan sug‘d madaniyati asta sekin tarix sahnasidan o‘chib keta boshladi.
Additional file 13 of Inclusion of a dual signal sequence enhances the immunogenicity of a novel viral vectored vaccine against the capsular group B meningococcus
Creators: name: Sheerin, Dylan
name: Dold, Christina
name: Silva-Reyes, Laura
name: Linder, Aline
name: Pollard, Andrew J.
name: Rollier, Christine S.
Publisher: name: figshare
Year: 2022
Subjects: subject: Immunology
subject: FOS: Clinical medicine

description: Additional file 13: Figure S1. Schematic of the N-terminal full-length signal sequence upstream of the factor H binding protein lipoprotein sequence.
Comparing a restorative justice school and a non-restorative justice school involved in consolidation: implications for perceptions of school climate
Creators: name: Oliveira, Philip
Publisher: name: No Publisher Supplied
Year: 2020

description: The current study compared student and staff perceptions of engagement of student voice, sense of community, and adult support within a Restorative Justice (RJ) school relative to a non-RJ school that did not have a restorative initiative. The study also considered the concept of “frame of reference” as it relates to perceived school climate. Specifically, the RJ and the non-RJ schools consolidated into a single high school. It was anticipated that the merged students and staff would draw on an RJ frame of reference, based on their prior school, as they rated their current non-RJ school climate. The study’s participants include 103 students and 28 staff from the RJ school, and 263 students and 25 staff from the non-RJ school. Students and staff completed 20-minute surveys. As expected, the regression analyses showed that students in the 2017 RJ school reported significantly higher engagement of student voice and adult support when compared to the students in the 2018 non-RJ school. Contrary to expectations, there were no significant differences in student-perceived sense of community. Also, there were no significant differences in staff-reported engagement of student voice, sense of community among students and adult support. Also, unexpectedly, the cohort of students in the 2018 non-RJ school who previously attended the 2017 RJ school did not experience engagement of student voice, sense of community and adult support differently than their peers that had only attended the non-RJ school. At the same time, responses to the open-ended survey questions suggest that, in the non-RJ school, there were few opportunities for expressing student voice.
Additional file 14 of Inclusion of a dual signal sequence enhances the immunogenicity of a novel viral vectored vaccine against the capsular group B meningococcus
Creators: name: Sheerin, Dylan
name: Dold, Christina
name: Silva-Reyes, Laura
name: Linder, Aline
name: Pollard, Andrew J.
name: Rollier, Christine S.
Publisher: name: figshare
Year: 2022
Subjects: subject: Immunology
subject: FOS: Clinical medicine

description: Additional file 14: Figure S2. Antigen-specific IgG titres in sera from mice immunised with human adenovirus vectors encoding N-terminal sequence variants of the factor H binding protein antigen. Groups of six BALB/c mice were immunised with a sub-optimal dose of 1 × 107 infectious units of one of the human adenovirus serotype 5 (AdHu5) vectors encoding factor H binding protein (fHbp) with N-terminal signal sequence variants or 1/10 of the human dose of 4CmenB as a comparator. Enzyme-linked immunosorbent assays were performed on serum samples taken at weeks (A) two, (B) four, (C) six, and (D) 14 post-immunisation to determine the titres of anti-fHbp IgG in sera. Statistical comparisons were made using a Mann-Whitney U-test. * p < 0.05; ** p < 0.01; *** p < 0.001.
Additional file 3 of Study protocol for a pragmatic cluster randomized controlled trial to improve dietary diversity and physical fitness among older people who live at home (the “ALAPAGE study”)
Creators: name: Bocquier, Aurélie
name: Jacquemot, Anne-Fleur
name: Dubois, Christophe
name: Tréhard, Hélène
name: Cogordan, Chloé
name: Maradan, Gwenaëlle
name: Cortaredona, Sébastien
name: Fressard, Lisa
name: Davin-Casalena, Bérengère
name: Vinet, Agnès
name: Verger, Pierre
name: Darmon, Nicole
name: Arquier, Valérie
name: Briclot, Guillaume
name: Chamla, Rachel
name: Cousson-Gélie, Florence
name: Danthony, Sarah
name: Delrieu, Karin
name: Dessirier, Julie
name: Féart, Catherine
name: Fusinati, Christine
name: Gazan, Rozenn
name: Gibert, Mélissa
name: Lamiraud, Valérie
name: Maillot, Matthieu
name: Nadal, Dolorès
name: Trotta, Christelle
name: Verger, Eric O.
name: Viriot, Valérie
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Physiology
subject: FOS: Biological sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Science Policy
subject: 111714 Mental Health
subject: FOS: Health sciences

description: Additional file 3. Diversity ALAPAGE Score: calculation method.
Additional file 15 of Inclusion of a dual signal sequence enhances the immunogenicity of a novel viral vectored vaccine against the capsular group B meningococcus
Creators: name: Sheerin, Dylan
name: Dold, Christina
name: Silva-Reyes, Laura
name: Linder, Aline
name: Pollard, Andrew J.
name: Rollier, Christine S.
Publisher: name: figshare
Year: 2022
Subjects: subject: Immunology
subject: FOS: Clinical medicine

description: Additional file 15: Figure S3. Intracellular and surface expression of human adenovirus-encoded factor H binding protein with a full-length N terminal signal sequence on HeLa cells after overnight infection. HeLa cells (1 × 106 per sample) were infected overnight with 5 × 108 infectious units of a human adenovirus serotype 5 (AdHu5) construct encoding the factor H binding protein (fHbp) with a full-length signal sequence and expression was quantified by flow cytometry after surface only, intracellular only, or both surface and intracellular staining of harvested cells with an anti-fHbp antibody (JAR5) and a fluorescently-tagged detection antibody. The y-axis corresponds to the percentage of total fluorescent (fHbp-expressing) HeLa cells after overnight infection. The vast majority of antigen is expressed within the cell at this timepoint.
Additional file 16 of Inclusion of a dual signal sequence enhances the immunogenicity of a novel viral vectored vaccine against the capsular group B meningococcus
Creators: name: Sheerin, Dylan
name: Dold, Christina
name: Silva-Reyes, Laura
name: Linder, Aline
name: Pollard, Andrew J.
name: Rollier, Christine S.
Publisher: name: figshare
Year: 2022
Subjects: subject: Immunology
subject: FOS: Clinical medicine

description: Additional file16: Figure S4. Time-course expression assay of human-adenovirus-encoded factor H binding protein with a full-length N-terminal signal sequence on HeLa cells stimulated with brefeldin. A. HeLa cells (1 × 106 per sample) were stimulated with brefeldin A to stop protein transport within the cells and subsequently infected with 5 × 108 infectious units of a human adenovirus serotype 5 (AdHu5) construct encoding the factor H binding protein (fHbp) with a full-length signal sequence and expression was quantified by flow cytometry after intracellular only staining of harvested cells with an anti-fHbp antibody (JAR5) and a fluorescently-tagged detection antibody. The y-axis corresponds to the percentage of total fluorescent (fHbp-expressing) HeLa cells after each hourly timepoint post-infection (x-axis) up to six hours. The antigen begins to be expressed at high levels at three hours post-infection and expression levels plateau around five hours.
Additional file 4 of Study protocol for a pragmatic cluster randomized controlled trial to improve dietary diversity and physical fitness among older people who live at home (the “ALAPAGE study”)
Creators: name: Bocquier, Aurélie
name: Jacquemot, Anne-Fleur
name: Dubois, Christophe
name: Tréhard, Hélène
name: Cogordan, Chloé
name: Maradan, Gwenaëlle
name: Cortaredona, Sébastien
name: Fressard, Lisa
name: Davin-Casalena, Bérengère
name: Vinet, Agnès
name: Verger, Pierre
name: Darmon, Nicole
name: Arquier, Valérie
name: Briclot, Guillaume
name: Chamla, Rachel
name: Cousson-Gélie, Florence
name: Danthony, Sarah
name: Delrieu, Karin
name: Dessirier, Julie
name: Féart, Catherine
name: Fusinati, Christine
name: Gazan, Rozenn
name: Gibert, Mélissa
name: Lamiraud, Valérie
name: Maillot, Matthieu
name: Nadal, Dolorès
name: Trotta, Christelle
name: Verger, Eric O.
name: Viriot, Valérie
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Physiology
subject: FOS: Biological sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Science Policy
subject: 111714 Mental Health
subject: FOS: Health sciences

description: Additional file 4. Food Frequency Questionnaire (FFQ).
Additional file 17 of Inclusion of a dual signal sequence enhances the immunogenicity of a novel viral vectored vaccine against the capsular group B meningococcus
Creators: name: Sheerin, Dylan
name: Dold, Christina
name: Silva-Reyes, Laura
name: Linder, Aline
name: Pollard, Andrew J.
name: Rollier, Christine S.
Publisher: name: figshare
Year: 2022
Subjects: subject: Immunology
subject: FOS: Clinical medicine

description: Additional file17: Figure S5. Side-by-side comparison of factor H binding protein N-terminal signal sequence variants, with and without enhanced green fluorescent protein tags, expressed from human adenovirus vectors after overnight infection HeLa cells. HeLa cells (1 × 106 per sample) were infected overnight with 5 × 108 infectious units of one of a series of human adenovirus serotype 5 (AdHu5) constructs encoding an N-terminal sequence variant of the factor H binding protein (fHbp), fHbp fused with enhanced green fluorescent protein (eGFP), or GFP only, and expression was quantified by flow cytometry after surface and intracellular staining of non-GFP-expressing cells with an anti-fHbp antibody (JAR5) and a fluorescently-tagged detection antibody. The y-axis corresponds to the percentage of total fluorescent (fHbp- and/or GFP-expressing) HeLa cells after overnight infection. The differences in expression levels between the antigen variants tested is conserved for the eGFP fusion antigens and lower for the eGFP fusion antigens than the eGFP only positive control, confirming that the differences in antigen expression levels are attributable to the fHbp N-terminal signal sequence.
THE INFLUENCE OF LEARNING STRATEGIES AND DIGITAL LITERACY OF CIVIC EDUCATION LEARNING OUTCOMES IN SENIOR HIGH SCHOOL - LIMA PULUH KOTA REGENCY, INDONESIA
Creators: name: Riza Desriandi
name: Azwar Ananda
name: Khairani, Erianjoni
Publisher: name: Zenodo
Year: 2024
Subjects: Influence, Learning Strategies, Digital Literacy, Learning Outcomes, Civic Education.

description: Abstract

This research was about the influence of learning strategies and digital literacy on learning outcomes in the civic education field of study at Senior High School (SHS) - Lima Puluh Kota Regency. This research aims to describe the factors that influence Civics learning outcomes, including: 1) the influence of learning strategies on Civics learning outcomes; and 2) the influence of digital literacy on civics learning outcomes. This type of research is quantitative research using quantitative data collection and analysis methods to answer the research problem formulation. The research population was all high school students who took part in citizenship lessons in the Limapuluh Kota district. Sampling in this research used the Multistage Sampling technique. Surveys and questionnaires were data collection techniques used in this research. To determine the validity of the instrument, Pearson Product Moment correlation analysis was used to calculate reliability using Cronbach's Alpha formula. The data analysis technique used in this research is the path analysis approach using the AMOS method, which previously required normality and linearity tests. Based on the research findings, it can be concluded that there was a significant influence between the research variables. There is the influence of learning strategies on civics learning outcomes and the influence of digital literacy on civics learning outcomes.
Additional file 5 of Study protocol for a pragmatic cluster randomized controlled trial to improve dietary diversity and physical fitness among older people who live at home (the “ALAPAGE study”)
Creators: name: Bocquier, Aurélie
name: Jacquemot, Anne-Fleur
name: Dubois, Christophe
name: Tréhard, Hélène
name: Cogordan, Chloé
name: Maradan, Gwenaëlle
name: Cortaredona, Sébastien
name: Fressard, Lisa
name: Davin-Casalena, Bérengère
name: Vinet, Agnès
name: Verger, Pierre
name: Darmon, Nicole
name: Arquier, Valérie
name: Briclot, Guillaume
name: Chamla, Rachel
name: Cousson-Gélie, Florence
name: Danthony, Sarah
name: Delrieu, Karin
name: Dessirier, Julie
name: Féart, Catherine
name: Fusinati, Christine
name: Gazan, Rozenn
name: Gibert, Mélissa
name: Lamiraud, Valérie
name: Maillot, Matthieu
name: Nadal, Dolorès
name: Trotta, Christelle
name: Verger, Eric O.
name: Viriot, Valérie
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Physiology
subject: FOS: Biological sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Science Policy
subject: 111714 Mental Health
subject: FOS: Health sciences

description: Additional file 5. Self-administered questionnaires’ sections related to process evaluation.
Additional file 6 of Study protocol for a pragmatic cluster randomized controlled trial to improve dietary diversity and physical fitness among older people who live at home (the “ALAPAGE study”)
Creators: name: Bocquier, Aurélie
name: Jacquemot, Anne-Fleur
name: Dubois, Christophe
name: Tréhard, Hélène
name: Cogordan, Chloé
name: Maradan, Gwenaëlle
name: Cortaredona, Sébastien
name: Fressard, Lisa
name: Davin-Casalena, Bérengère
name: Vinet, Agnès
name: Verger, Pierre
name: Darmon, Nicole
name: Arquier, Valérie
name: Briclot, Guillaume
name: Chamla, Rachel
name: Cousson-Gélie, Florence
name: Danthony, Sarah
name: Delrieu, Karin
name: Dessirier, Julie
name: Féart, Catherine
name: Fusinati, Christine
name: Gazan, Rozenn
name: Gibert, Mélissa
name: Lamiraud, Valérie
name: Maillot, Matthieu
name: Nadal, Dolorès
name: Trotta, Christelle
name: Verger, Eric O.
name: Viriot, Valérie
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Physiology
subject: FOS: Biological sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Science Policy
subject: 111714 Mental Health
subject: FOS: Health sciences

description: Additional file 6. Self-administered questionnaires’ section related to participant’s characteristics.