Additional file 1: of The influence of the size and aspect ratio of anisotropic, porous CaCO3 particles on their uptake by cells
Creators: name: Parakhonskiy, Bogdan
name: Zyuzin, Mikhail
name: Yashchenok, Alexey
name: Carregal-Romero, Susana
name: Rejman, Joanna
name: Möhwald, Helmuth
name: Parak, Wolfgang
name: Skirtach, Andre
Publisher: name: Figshare
Year: 2015
Subjects: subject: Space Science
subject: 29999 Physical Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: FOS: Physical sciences
subject: Microbiology
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Cell Biology
subject: Molecular Biology
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: FOS: Chemical sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: FOS: Clinical medicine
subject: Hematology
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: FOS: Health sciences
subject: 60506 Virology

description: Figure S1. Cumulative probability p(N) for the total internalized particle volume Vt per cell (p(Vt)). Particles had different shape (and size): “small” ellipsoidal CaCO3 (black dots), “big” ellipsoidal CaCO3 (red dots), cuboidal CaCO3 (blue dots), “small” spherical CaCO3 (green dots) and “big” spherical SiO2 (pink dots). The plot is based on the data shown in Figure 9. The vertical bars represent the standard deviation values. Figure S2. Parameters of particles and cumulative probability. (a) Volume (V), surface (S) and surface-to-volume ratio (S/V) of the five different kinds of particles. (b) Cumulative probability plot p(St) for total surface St of internalized particles per cell for particles with different shape (and size) after 24 h of particle incubation with a concentration of 10 particles added per cell. The total internal surface corresponds to the surface S of the particles times the number N of internalized particles per cell. Data are displayed for cuboidal CaCO3 (blue dots), “small” spherical CaCO3 (green dots), “big” spherical SiO2 (pink dots), “big” ellipsoidal CaCO3 (red dots), and “small” CaCO3 ellipsoidal particles (black dots). All particles had the same surface modification (positively charged layer of PAH). The vertical bars represent the standard deviation values. Table SI-1. Raw data of the histogram shown in Figure 5a for "small" and "big" ellipsoidal particles. f(N) is the frequency of cells which have internalized N particles per HeLa cell after 24 h of particle incubation with a concentration of 10 added particles per cell. SD stands for standard deviation. Table SI-2. Raw data of the cumulative probability plot shown in Figure 5b for "small" and "big" ellipsoidal particles. p(N) is the cumulative probability of cells, which have internalized N particles per HeLa cell after 24 h of particle incubation with a concentration of 10 added particles per cell. SD stands for standard deviation. Table SI-3. Raw data of the cumulative probability plot shown in Figure 5c for all types of particles. p(N) is the cumulative probability of cells, which have internalized N particles per HeLa cell after 24 h of particle incubation with a concentration of 10 added particles per cell. SD stands for standard deviation, sph. stands for spherical, ellip. stands for ellipsoidal and cub. stands for cuboidal. Table SI-4. Raw data of the cumulative total internalized particle volume Vt per cell (p(Vt)) shown in Figure S1. SD stands for standard deviation, sph. stands for spherical, ellip. stands for ellipsoidal and cub. stands for cuboidal.