title: ÐÐµÑÐ¾Ð´Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ Ð¾ÑÐ¾Ð±ÐµÐ½Ð½Ð¾ÑÑÐ¸ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¸ Ð²ÑÑÐ²Ð»ÐµÐ½Ð¸Ñ Ð²Ð¸ÑÑÑÐ¾Ð² Ð»ÐµÐ¹ÐºÐ¾Ð·Ð° Ð¿ÑÐ¸Ñ RAV-1 Ð¸ RAV-2
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Methodological features of cultivation and detection of avian leukosis viruses RAV-1 and RAV-2
Creators: name: ÐÐ¾Ð»ÑÑÐ°ÐºÐ¾Ð²Ð°, ÐÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÐÐ¸ÑÑÑÐ¾Ð»Ð¾Ð³Ð¸Ñ
Ð²Ð¸ÑÑÑ Ð»ÐµÐ¹ÐºÐ¾Ð·Ð° Ð¿ÑÐ¸Ñ (ÐÐÐ)
ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÐÐÐ
Ð¾Ð±ÑÐ¸Ð¹ Ð°Ð½ÑÐ¸Ð³ÐµÐ½ p27
avian leukosis virus (ALV)
cultivation of ALV
total antigen p27

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¿Ð¾Ð´Ð±Ð¾ÑÑ Ð¾Ð¿ÑÐ¸Ð¼Ð°Ð»ÑÐ½ÑÑ ÑÑÐ»Ð¾Ð²Ð¸Ð¹ Ð¸ ÑÐ¸ÑÑÐµÐ¼Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð²Ð¸ÑÑÑÐ¾Ð² Ð»ÐµÐ¹ÐºÐ¾Ð·Ð° Ð¿ÑÐ¸Ñ RAV-1 Ð¸ RAV-2 Ð¸ Ð¼ÐµÑÐ¾Ð´Ð°Ð¼ Ð²ÑÑÐ²Ð»ÐµÐ½Ð¸Ñ Ð²Ð¸ÑÑÑÐ¾Ð² Ð»ÐµÐ¹ÐºÐ¾Ð·Ð° Ð¿ÑÐ¸Ñ. ÐÐµÑÐ¾Ð´Ð¾Ð¼ ÐÐ¤Ð Ð±ÑÐ» Ð²ÑÑÐ²Ð»ÐµÐ½ Ð¾Ð±ÑÐ¸Ð¹ Ð°Ð½ÑÐ¸Ð³ÐµÐ½ ÐÐÐ p27 Ð² ÑÑÐ²Ð¾ÑÐ¾ÑÐºÐ°Ñ ÐºÑÑ Ð¿Ð¾ÑÐ¾Ð´ Ð ÑÑÑÐºÐ°Ñ Ð±ÐµÐ»Ð°Ñ Ð¸ ÐÐ¼ÑÐ¾ÐºÑ Ñ ÑÐ°ÑÑÐ¾ÑÐ¾Ð¹: 0,43 Ð¸ 0,54 (ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²ÐµÐ½Ð½Ð¾). ÐÐ½ÑÐ¸Ð³ÐµÐ½ p27 ÑÐ°ÐºÐ¶Ðµ Ð±ÑÐ» Ð²ÑÑÐ²Ð»ÐµÐ½ Ð² Ð¿Ð¾Ð»ÑÐ¿ÑÐ¾Ð´ÑÐºÑÐ°Ñ Ð³ÑÐ¸Ð¿Ð¿Ð¾Ð·Ð½ÑÑ Ð²Ð°ÐºÑÐ¸Ð½. ÐÑÑÐ¾ÐºÐ°Ñ ÑÐ°ÑÑÐ¾ÑÐ° Ð²ÑÑÐ²Ð»ÐµÐ½Ð¸Ñ ÐÐÐ Ð¿Ð¾Ð´ÑÑÑÐºÐ¸Ð²Ð°ÐµÑ Ð²Ð°Ð¶Ð½Ð¾ÑÑÑ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ¸ ÑÑÐ°Ð½Ð´Ð°ÑÑÐ¸Ð·Ð¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ ÑÐ¿Ð¾ÑÐ¾Ð±Ð° Ð¸Ð½Ð°ÐºÑÐ¸Ð²Ð°ÑÐ¸Ð¸ Ð²Ð¸ÑÑÑÐ¾Ð² Ð³ÑÐ¸Ð¿Ð¿Ð° Ð¾Ð´Ð½Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾ Ñ ÐºÐ¾Ð½ÑÐ°Ð¼Ð¸Ð½Ð°Ð½ÑÐ°Ð¼Ð¸ Ð¿ÑÐ¸ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´ÑÑÐ²Ðµ Ð³ÑÐ¸Ð¿Ð¿Ð¾Ð·Ð½ÑÑ Ð²Ð°ÐºÑÐ¸Ð½. ÐÐ»Ñ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ¾Ð² Ð¿Ð¾ ÐºÐ¾Ð½ÑÑÐ¾Ð»Ñ Ð¸Ð½Ð°ÐºÑÐ¸Ð²Ð°ÑÐ¸Ð¸ ÐÐÐ ÑÑÐ°Ð½Ð´Ð°ÑÑÐ½ÑÐ¼Ð¸ Ð¸Ð½Ð°ÐºÑÐ¸Ð²Ð¸ÑÑÑÑÐ¸Ð¼Ð¸ Ð°Ð³ÐµÐ½ÑÐ°Ð¼Ð¸ Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼Ñ Ð¾ÑÑÐ°Ð±Ð¾ÑÐ°Ð½Ð½ÑÐµ Ð¼ÐµÑÐ¾Ð´Ñ Ð¸Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¸ Ð²ÑÑÐ²Ð»ÐµÐ½Ð¸Ñ. Ð ÑÐ°Ð¼ÐºÐ°Ñ Ð½Ð°ÑÑÐ¾ÑÑÐµÐ¹ ÑÐ°Ð±Ð¾ÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÑ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ RAV-1 Ð¸ RAV-2: ÑÐ¸ÑÑÐµÐ¼Ð° ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ, Ð¿Ð°ÑÑÐ°Ð¶ Ð²Ð¸ÑÑÑÐ°, Ð´Ð»Ð¸ÑÐµÐ»ÑÐ½Ð¾ÑÑÑ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ, ÑÐ¸ÑÐ»Ð¾ Ð·Ð°Ð¼Ð¾ÑÐ°Ð¶Ð¸Ð²Ð°Ð½Ð¸Ð¹/Ð¾ÑÑÐ°Ð¸Ð²Ð°Ð½Ð¸Ð¹. ÐÐ¿ÑÐ¸Ð¼Ð°Ð»ÑÐ½ÑÐ¼Ð¸ ÑÑÐ»Ð¾Ð²Ð¸ÑÐ¼Ð¸ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ RAV-1 Ð¸ RAV-2 ÑÐ²Ð»ÑÐ»Ð¸ÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¿ÐµÑÐ²Ð¸ÑÐ½Ð¾Ð¹ ÐºÑÐ»ÑÑÑÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐ¸Ð±ÑÐ¾Ð±Ð»Ð°ÑÑÐ¾Ð² ÑÐ¼Ð±ÑÐ¸Ð¾Ð½Ð¾Ð² ÐºÑÑ (Ð¤Ð­Ð) Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ´Ñ 199, Ð·Ð°ÑÐ°Ð¶ÐµÐ½Ð¸Ðµ ÐºÐ»ÐµÑÐ¾Ðº 3-Ð¼ Ð¸Ð»Ð¸ 2-Ð¼ Ð¿Ð°ÑÑÐ°Ð¶ÐµÐ¼, ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð² ÑÐµÑÐµÐ½Ð¸Ðµ 12 Ð¸Ð»Ð¸ 7 ÑÑÑÐ¾Ðº ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²ÐµÐ½Ð½Ð¾ Ð¸ Ð´Ð²ÑÐºÑÐ°ÑÐ½Ð¾Ðµ Ð·Ð°Ð¼Ð¾ÑÐ°Ð¶Ð¸Ð²Ð°Ð½Ð¸Ðµ/Ð¾ÑÑÐ°Ð¸Ð²Ð°Ð½Ð¸Ðµ Ð¿ÑÐ¾Ð±. Ð ÑÐ¾Ð´Ðµ Ð¾ÑÑÐ°Ð±Ð¾ÑÐºÐ¸ Ð¼ÐµÑÐ¾Ð´Ð° Ð±Ð»ÑÑÐºÐ¾Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ñ Ð´Ð»Ñ Ð²Ð¸ÑÑÑÐ° RAV-1 Ð±ÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾ Ð¾ÑÑÑÑÑÑÐ²Ð¸Ðµ ÑÐ¸ÑÐ¾Ð¿Ð°ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ð¸ ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð±Ð»ÑÑÐµÐº. Ð¢Ð¸ÑÑ Ð²Ð¸ÑÑÑÐ° Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÑÐ»Ð¸ ÑÐ¸ÑÑÐ¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð½Ð° Ð¤Ð­Ð Ñ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐµÐ¹ Ð¿Ð¾ÑÑÐ°Ð½Ð¾Ð²ÐºÐ¾Ð¹ Ð²Ð¸ÑÑÑÑÐ¾Ð´ÐµÑÐ¶Ð°ÑÐµÐ¹ ÐºÑÐ»ÑÑÑÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð¶Ð¸Ð´ÐºÐ¾ÑÑÐ¸ Ð² ÐÐ¤Ð. ÐÑÐ» Ð¿Ð¾Ð»ÑÑÐµÐ½ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°Ñ RAV-1 Ñ ÑÐ¸ÑÑÐ¾Ð¼ Ð½Ðµ Ð¼ÐµÐ½ÐµÐµ 8lg. Ð ÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð½Ð°ÑÑÐ¾ÑÑÐµÐ¹ ÑÐ°Ð±Ð¾ÑÑ Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÑ Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸ÑÑ Ð½Ð°ÐºÐ¾Ð¿Ð»ÐµÐ½Ð¸Ðµ ÐÐÐ Ð´Ð»Ñ Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐµÐ¹ Ð¾ÑÑÐ°Ð±Ð¾ÑÐºÐ¸ ÑÐ¿Ð¾ÑÐ¾Ð±Ð¾Ð² Ð¸ ÑÑÐ»Ð¾Ð²Ð¸Ð¹ ÐµÐ³Ð¾ Ð¸Ð½Ð°ÐºÑÐ¸Ð²Ð°ÑÐ¸Ð¸.
description: The given work is devoted to the selection of optimal conditions and systems of avian leukosis viruses RAV-1 and RAV-2 cultivation and detection methods. By ELISA method, the total ALV p27 antigen was detected in the sera of Russian White and Amrox chicken breeds with frequencies: 0.43 and 0.54 (respectively). The p27 antigen was also detected in the influenza vaccine intermediates. The high frequency of ALV detection emphasizes the importance of developing a standardized method of inactivation of influenza viruses simultaneously with contaminants during production of influenza vaccines. Experiments to control ALV inactivation with standard inactivating agents require well-established methods for cultivation and detection of ALV. The following cultivation parameters of RAV-1 and RAV-2 were investigated in this work: cultivation system, passage of the virus, duration of cultivation, number of freezing/thawing. Optimal cultivation conditions for RAV-1 and RAV-2 were the use of primary culture of chicken embryo fibroblast (CEF) using nutrient medium 199, infection of cells with the 3rd or the 2nd passages, cultivating for 12 or 7 days, respectively, and freezing/thawing samples for 2 times. The absence of cytopathic effect and plaque formation was demonstrated during the plaque-forming methods for RAV-1 virus. The virus titer was determined by titration in CEF followed by ELISA for p27 detection in viral-containing cultural liquid. A RAV-1 concentrate with a titer of at least 8lg was obtained. The results of this work will allow to accumulate ALV for further development of methods and conditions of its inactivation.