Calycina montana Lestari & Thilini Chethana 2022, comb. nov.
Creators: name: Lestari, Anis S.
name: Thilini Chethana, K. W.
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Fungi
Ascomycota
Leotiomycetes
Rhytismatales
Pezizellaceae
Calycina
Calycina montana

description: Calycina montana (W.Y. Zhuang & H.D. Zheng) Lestari & Chethana, comb. nov. (FIGURE 2–4)   
 Basionym: Bisporella montana W.Y. Zhuang & H.D. Zheng, Zhuang, Zheng & Ren, Mycosystema 36(4): 407 (2017)   
Index Fungorum number: IF 570309, Facesoffungi number: FoF 10812  
Holotype: HMAS 275566  
 Saprobic on decayed woods covered with mosses. Sexual morph: Apothecia 1.1–1.6 × 0.6–1.4 mm, gregarious, arising singly or in clusters, amber colored, stipitate. Stipe 0.6–1.2 x 0.2–0.4 mm in dried condition. Receptacle discoid, bright amber color to hyaline. Margin hyaline with brown outer layer at the flank. Disc concave. Ectal excipulum 55–68 µm (= 59.8 µm, n = 10) at the flank near the stipe, composed of gelatinized thick-walled, hyaline cells of textura porrecta. Medullary excipulum 36–80 µm (= 57.7 µm, n = 10) at the flank near the stipe, distinctively separate from ectal excipulum, composed of thin-walled, hyaline cells of textura oblita, hypha with refractive guttules. Hymenium 141– 180 µm (= 168.9 µm, n = 15), amber colored. Paraphyses 1.8–2.5 µm in diam. (= 2.1 µm, n = 15) at the terminal, filiform, septate, branched at the 1/3 length from the base, guttules. Asci 101–118 × 4.7–6.3 µm (= 108.6 × 5.5 µm, n = 15), unitunicate, 8-spored, cylindrical and subclavate at the top, rounded (in alive state) to conical apex (dead state), J+ (eu-amyloid, in Melzer’s with and without KOH treatment), long substipitate base, arising from croziers. Ascospores 7.1–9.2 × 4.1–5 µm (= 8 × 4.4 µm, n = 20), ellipsoid, 0-1 septate, partially biseriate to uniseriate, hyaline, rounded at both ends with guttules. Asexual morph: Hypomycetous, on the lower flank and receptacle of the apothecia’s surface. Conidiogenous cells 12–19 × 3.7–5.1 (= 14.3 × 4.3 µm, n = 10) µm, phialidic, black to dark brown. Conidiophore 1.5–3.7 × 1.6–2.4 (= 3 × 1.9, n = 5) µm, black to dark brown. Conidia 3.2–3.8 × 1.7–2 (= 3.5 × 1.9 µm, n = 2) µm ellipsoid, aseptate, hyaline, smooth.   
 Material examined: Thailand, Chiang Mai Province, Chomthong District, Doi Inthanon, Kew Mae Pan nature trail, 20 October 2021, Anis S. Lestari, KMP3 21 (MFLU 22-0055), KMP3 41 (MFLU 22-0056) (Figure 2 & 3); CHINA, Yunnan, Shangrila, Pingbian, Daweishan on rotten hard wood, 5 September 1999, X.Q. Zhang & D.Z. Ren, (HMAS 275566, holotype) (Figure 4).   
GenBank numbers: MFLU 22-0055: ITS – ON156524, LSU – ON156523; MFLU 22-0056: ITS – ON176689, LSU – ON325438  
 Notes: In the current study, Bisporella montana (HMAS 275566, holotype) and our specimens clustered under Calycina, as Calycina montana. Calycina montana (HMAS 275566), previously known as Bisporella montana, was discovered in a mountainous area in China as a saprobe on woody substrates (Zhuang et al. 2017). The characters of both specimens, especially the amber-colored apothecia and gelatinized textura porrecta cells of ectal excipulum, fit the generic concept of Calycina (Baral 1993). Another character which further confirms the combination is the appearance of a calycina-like apical ring shown clearly in our collection (Figure 3j) and the type specimen (Figure 4m). Based on phylogenetic analyses using ITS and LSU sequence data, our collection clusters with the type strain with high statistical support of 99% ML and 0.99 BI (Figure 1). Across the 466 base pairs of ITS (+ 5.8S) gene of our Calycina montana strains (MFLU 22-0055 and MFLU 22-0056) and the ex-type HMAS 275566, only one base pair difference (0.22 %) was observed.
description: Published as part of Lestari, Anis S. & Thilini Chethana, K. W., 2022, Morpho-phylogenetic insights reveal Bisporella montana as Calycina montana comb. nov. (Pezizellaceae, Helotiales), pp. 185-202 in Phytotaxa 558 (2) on pages 190-193, DOI: 10.11646/phytotaxa.558.2.3, http://zenodo.org/record/7002943