cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
title: Seed dispersal effectiveness of samango monkey (Cercopithecus albogularis schwarzi)
Creators: name: Stringer, S
Publisher: name: Liverpool John Moores University
Year: 2022

description: The effectiveness of a seed disperser is dependent on the impact it has on plant fitness. For fruiting species, plant fitness is dependent on the behaviour of its mutualists in (a) reducing seed mortality and (b) increasing the likelihood of future reproduction. The main aim of this thesis was to assess how intergroup variability in the feeding and movement behaviour of a highly social frugivore, influences seed dispersal effectiveness (SDE) and plant fitness. I achieved this by deconstructing the different components of seed dispersal, the quality of dispersal and the quantity of dispersal and investigated how the behaviour of samango monkeys influenced each component. Germination experiments demonstrated that although removal of germination inhibiting fruit pulp through seed-spitting increased germination potential compared to the mechanical and scarification of seeds via seed-swallowing, plants may trade quality for quantity, as the SDE of samango monkeys was greater for swallowed seeds. Time budget analysis showed that intergroup variability in feeding behaviour led to differences in the quantity of seeds dispersed by each group Movement behaviour analysis showed that differences in habitat quality influenced the quality of dispersal. Gut passage time analysis provided a reliable estimate of a gut passage time window between 16.63 – 25.12 hrs. The findings of this study highlight intergroup variability in SDE of neighbouring groups of social foraging frugivores, which possibly arose through ecological constraints associated with group size (Janson & van Schaik 1988; Chapman & Chapman 2000b). Intergroup variability in SDE could have important consequences on the maintenance of forest systems and the recruitment in, and colonization of, secondary forest or open habitats. Variation in SDE within animal populations can have important implications for spatial demographics in plant communities, and this thesis highlights the importance of including intergroup variability seed dispersal models.
Chemical Polymorphism and Composition of Leaf Essential Oils of Cinnamomum kanehirae Using Gas Chromatography/Mass Spectrometry, Cluster Analysis, and Principal Component Analysis
Creators: name: Chen, Ying-Ju
name: Sen-Sung Cheng
name: Chun-Ya Lin
name: Chih-Kai Yang
name: Min-Jay Chung
name: Shang-Tzen Chang
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Inorganic Chemistry
subject: FOS: Chemical sciences
subject: Biological Sciences
subject: Ecology
subject: FOS: Biological sciences
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Pharmacology
subject: Cell Biology
subject: Microbiology
subject: Biochemistry

description: Cinnamomum kanehirae Hayata is an endemic tree species in Taiwan. Leaf essential oils from 26 sources of C. kanehirae were obtained by hydrodistillation and their chemical constituents were analyzed by gas chromatography-mass spectrometry (GC-MS). Fifty-eight compounds were identified from C. kanehirae leaf essential oils. The main constituents in leaf essential oils were linalool, 1,8-cineole, β-selinene, 1-hexadecyne, and α-cadinol. According to the chemical compositions of leaf essential oils and their relative contents obtained from GC-MS analyses, cluster analysis, and principal component analysis, the 26 sources examined were classified into five chemotypes: linalool type, linalool/1,8-cineole type, 1,8-cineole type, linalool/α-cadinol type, and mixed type. The biochemical correlations between the major constituents of C. kanehirae were examined and their relationship is discussed.
ABO phenotype protected reproduction as it is based on specific fucosylations.
Creators: name: Arend, Peter
Publisher: name: figshare
Year: 2016
Subjects: subject: Hematology
subject: Medicine
subject: Biochemistry
subject: Cancer
subject: Biological Techniques
subject: Immunology
subject: FOS: Clinical medicine
subject: Computational Chemistry
subject: Molecular Biology
subject: Stem Cells
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Anatomy

description: The molecular biological relations between the formation of the ABO(H) blood group phenotype and human fertility is evident in the rare (Oh) or Bombay blood type, which Charles Darwin would have interpreted as resulting from reduced fertility in consanguinities, based on the history of his own family, the Darwin/Wedgewood Dynasty. In its native form, the Bombay type occurs in individuals with an extremely rare genotype (h/h; se/se), which does not produce the fucosyltransferases FUT1and FUT2 encoded on chromosome 19 due to point mutations. In fact, the Bombay type individual cannot synthesize the fucosyl residues or H- receptor, which in epistatic connection with the A- and B-allelic glycotransferases, encoded on chromosome 9, provides the structural basis for the formation of ABO(H) phenotypes on the cell surfaces, secretions and plasma proteins, such as van Willebrand factor (vWF), clotting factor VIII and the α2-macroglobulin (A2M). The innate isoagglutinins instead show extremely high levels of a mercaptoethanol-sensitive, complement-binding anti-H reactivity, acting over a wide range of temperatures, with an amplitude at 370C. This reactivity appears to be dominated by immunoglobulin M (IgM), which in view of the role of immunoglobulin fucosylation and the induction of antibody-dependent cellular cytotoxicity (ADCC) by defucosylated immunoglobulin G (IgG), hypothetically promotes anti-self-reactive ADCC, that although not mediated by normal IgM, may be mediated by defucosylated IgM. Alternatively, the unusual temperature amplitude of the anti-H-reactive IgM, characteristic for Bombay type plasma, highly suggests the involvement of IgG. Moreover, most examples of IgM apparently have a significant IgG component. While the anti-H reactivity of the Bombay type plasma thus indicates the functions of several immunoglobulin classes, the defucosylation of at least the IgM should be genetically identical with that of the cell surfaces, and thus also results from the lack of FUT1/FUT2 encoding on chromosome 19. In fact, the FUT1/FUT2-based ABO(H)-reactivity, expressed by the A2M, is depleted, which synergistically works with the structurally related, ancestral IgM molecule. Consequently, in the Bombay type individual, the non-somatic glycosylation processes, which occur during embryogenic stem cell-to-germ cell transformation, are exposed to poorly fucosylated, glycan-depleted immunoglobulins initiating a complex anti-self-reactivity in male gamete performances. 
O'ZBEKISTON RESPUBLIKASI QONUNCHILIGINING RIVOJLANISH TEENDENSIYALARI
Creators: name: Jomurodov, Jasurbek
Publisher: name: Zenodo
Year: 2024

description: Qonunchilik: 1) davlatning oʻz oldida turgan vazifa va funksiyalarini amalga oshirish usullaridan biri. Bu vakolatli davlat organlari (parlament) tomonidan davlat va jamiyat hayotining eng muhim masalalari boʻyicha qonunlar qabul qilish faoliyatida ifodalanadi (qarang Qonun chiqaruvchi hokimiyat);
Water Activities and Osmotic Coefficients of Aqueous Solutions of Five Alkylaminium Sulfates and Their Mixtures with H2SO4 at 25oC
Creators: name: Sauerwein, Meike
name: Clegg, Simon L.
name: Chak K. Chan
Publisher: name: Taylor & Francis
Year: 2015
Subjects: Astronomy, Astrophysics, Space Science
Biological Sciences
Chemistry
Earth and Environmental Sciences
Engineering
Biochemistry

description: Alkylaminium sulfates are frequently detected in ambient aerosols, and are believed to be important in the nucleation of new particles in the atmosphere, despite the comparatively low gas phase concentrations of amines. In this study, water activities and osmotic coefficients have been measured, using a chilled mirror dew point technique, of aqueous mixtures of sulfuric acid and the following alkylaminium sulfates: methylaminium, ethylaminium, dimethylaminium, diethylaminium, and trimethylaminium sulf-ate. The samples were prepared by mixing solutions of the five corresponding amines and aqueous sulfuric acid and determining the exact aminium to sulfate molar ratios by ion chromatography. The results were correlated using an extended Zdanovskii–Stokes–Robinson equation to enable concentration/water activity rela-tionships to be calculated over the entire composition range from pure aqueous sulfuric acid to pure aqueous aminium sulfate. Water activities and osmotic coefficients for aminium:sulfate ratios of 1:1 (the bisulfate salts) and lower showed great similarity with ammonium bisulfate, but osmotic coefficients for the 2:1 ratio (the aminium sulfates) were significantly larger (and water activities lower) than for ammonium sulfate. These results differ from those obtained in Clegg et al.'s (2013) study. The relative values of the osmotic coefficients, in concentrated solutions, suggest that the numbers of methyl or ethyl groups in the aminium ion may have a stronger lowering effect on water activity than the alkyl chain length.Copyright 2015 American Association for Aerosol Research
ABO phenotype protected reproduction as it is based on specific fucosylations.
Creators: name: Arend, Peter
Publisher: name: figshare
Year: 2016
Subjects: subject: Hematology
subject: Medicine
subject: Biochemistry
subject: Cancer
subject: Biological Techniques
subject: Immunology
subject: FOS: Clinical medicine
subject: Computational Chemistry
subject: Molecular Biology
subject: Stem Cells
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Anatomy

description: The molecular biological relations between the formation of the ABO(H) blood group phenotype and human fertility is evident in the rare (Oh) or Bombay blood type, which Charles Darwin would have interpreted as resulting from reduced fertility in consanguinities, based on the history of his own family, the Darwin/Wedgewood Dynasty. In its native form, the Bombay type occurs in the extremely rare genotype (h/h; se/se), which does not synthesize the fucosyltransferases FUT and FUT2 encoded on chromosome 19 due to point mutations; it thus cannot accomplish the epistatic ABO(H)-allelic cooperation with chromosome 9, ABO(H) phenotype does not develop, and in view of the reported, pronounced antibody-dependent cellular cytotoxicity (ADCC), exerted by non- or defucosylated immunoglobulins, the high levels of complement-binding anti-H-reactivity, characterizing Bombay type plasma, are thought to reflect a genetically identical non-fucosylation of plasma proteins. In fact, the FUT1/FUT2-based ABO(H) reactivity, expressed by α2-macroglobulin (A2M) synergistically working with the structurally related, innate ancestral IgM, is also depleted in Bombay type plasma, lacking the ABO(H) phenotype-providing fucosylation of proteins. Consequently, non-fucosylated sites of the ancestral IgM and/or other immunoglobulins suggest to interact with developmental O-GalNAc glycosylations dominating the ESC-GC transformations, and thus promote a pronounced ADCC affecting male gamete performances.  
Sustainable Synthesis of the Naturally Hypolipidemic Agent α-Asarone
Creators: name: Camacho-Dávila, Alejandro A.
name: Chávez-Flores, David
name: Zaragoza-Galán, Gerardo
name: Ramos-Sánchez, Víctor H.
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Science Policy
subject: Biological Sciences
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Evolutionary Biology
subject: FOS: Biological sciences
subject: Biotechnology
subject: Pharmacology
subject: Molecular Biology
subject: Cell Biology
subject: Biochemistry

description: A short and practical preparation of α-asarone was developed using the inexpensive methylisoeugenol as a starting material. The utilization of a sequence of tribromination, debromination, and copper-mediated aromatic substitution enabled the stereoselective formation of only the E-isomer of α-asarone in good yield.
Water Activities and Osmotic Coefficients of Aqueous Solutions of Five Alkyl-Aminium Sulfates and Their Mixtures with H2SO4 at 25 °C
Creators: name: Sauerwein, Meike
name: Clegg, Simon L.
name: Chak K. Chan
Publisher: name: Taylor & Francis
Year: 2015
Subjects: Astronomy, Astrophysics, Space Science
Biological Sciences
Chemistry
Earth and Environmental Sciences
Engineering
Biochemistry

description: Alkylaminium sulfates are frequently detected in ambient aerosols, and are believed to be important in the nucleation of new particles in the atmosphere, despite the comparatively low gas phase concentrations of amines. In this study water activities and osmotic coefficients have been measured, using a chilled mirror dew point technique, of aqueous mixtures of sulfuric acid and the following alkylaminium sulfates: methylaminium, ethylaminium, dimethylaminium, diethylaminium and trimethylaminium sulfate. The samples were prepared by mixing solutions of the five corresponding amines and aqueous sulfuric acid and determining the exact aminium to sulfate molar ratios by ion chromatography. The results were correlated using an extended Zdanovskii-Stokes-Robinson equation to enable concentration/water activity relationships to be calculated over the entire composition range from pure aqueous sulfuric acid to pure aqueous aminium sulfates. Water activities and osmotic coefficients of aminium:sulfate ratios of 1:1 (the bisulfate salts) and lower showed great similarity with ammonium bisulfate, but osmotic coefficients for the 2:1 ratio (the aminium sulfates) were significantly larger (and water activities lower) than for ammonium sulfate. These results differ from those obtained in another recent study (S. L. Clegg, C. Qiu, and R. Zhang [2013]Atmos. Environ. 73:145–158). The relative values of the osmotic coefficients, in concentrated solutions, suggest that the numbers of methyl or ethyl groups in the aminium ion may have a stronger lowering effect on water activity than the alkyl chain length.
ABO phenotype protected reproduction based on specific fucosylations and explained by Bombay type formation.
Creators: name: Arend, Peter
Publisher: name: figshare
Year: 2016
Subjects: subject: Hematology
subject: Medicine
subject: Biochemistry
subject: Cancer
subject: Biological Techniques
subject: Immunology
subject: FOS: Clinical medicine
subject: Computational Chemistry
subject: Molecular Biology
subject: Stem Cells
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Anatomy

description: The molecular biological relations between the formation of the ABO(H) blood group phenotype and human fertility is evident in the rare (Oh) or Bombay blood type, which Charles Darwin would have interpreted as resulting from reduced fertility in consanguinities, based on the history of his own family, the Darwin/Wedgewood Dynasty. In its native form, the Bombay type occurs in the extremely rare genotype (h/h; se/se), which does not synthesize the fucosyltransferases FUT1 and FUT2 encoded on chromosome 19 due to point mutations; it thus cannot accomplish the epistatic ABO(H)-allelic cooperation with chromosome 9, ABO(H) phenotype does not develop, and in view of the reported, pronounced antibody-dependent cellular cytotoxicity (ADCC), exerted by non- or defucosylated immunoglobulins, the high levels of complement-binding anti-H-reactivity, characterizing Bombay type plasma, are thought to reflect a genetically identical non-fucosylation of plasma proteins. In fact, the FUT1/FUT2-based ABO(H) reactivity, expressed by α2-macroglobulin (A2M) synergistically working with the structurally related, innate ancestral IgM, is also depleted in Bombay type plasma, lacking the ABO(H) phenotype-providing fucosylation of proteins. Consequently, non-fucosylated sites of the ancestral IgM and/or other immunoglobulins suggest to interact with developmental O-GalNAc glycosylations dominating the ESC-GC transformations, and thus promote a pronounced ADCC affecting male gamete performances.  
title: Physical literacy assessment amongst young children
Creators: name: Goss, HR
Publisher: name: Liverpool John Moores University
Year: 2021

description: Physical literacy is defined as the motivation, confidence, physical competence and knowledge and understanding to value and engage in physical activity for life. The concept has increased in popularity in recent years, particularly within childhood. However, this popularity has preceded empirical evidence for the concept. It remains unclear how best to asses an individual’s physical literacy journey, which is crucial to provide evidence to support pedagogy and accountability. The aim of this thesis was to inform the development of a rigorous, aligned, and feasible physical literacy assessment tool for use in young children, aged 5-7 years old. Study One (Chapter Three) was a narrative review which aimed to clarify similarities, differences, and potential causes of contention across prominent international perspectives of physical literacy. It was recommended that work related to physical literacy should be transparent, enabling others to compare different interpretations and evaluate the effectiveness of intervention programs, policies and assessment. Study Two (Chapter Four) used rigorous protocol to conduct a large-scale systematic review of existing assessments related to physical literacy used in young children. 27 assessments: affective (n=7), physical (n=15), cognitive (n=6), were identified, with one assessment appraised in both the affective and cognitive domains. Findings offered detailed insight regarding the measurement properties, feasibility and alignment to physical literacy amongst existing assessments. Study Three (Chapter Five) explored stakeholders’ perceptions physical literacy assessment. Concurrent focus groups were conducted with academics/practitioners (n=21), teachers (n=23) and 5-7-year-old children (n=39). Findings demonstrated that although participants indicated demand for an assessment, current existing assessments do not meet the needs of stakeholders, and various recommendations regarding implementation were identified. This is the first study to qualitatively investigate stakeholders’ perceptions of physical literacy assessment. The findings of these studies and external research have informed the development of 10 recommendations for the assessment of physical literacy in younger children, presented in Chapter Six. We hope the empirical evidence reported within this thesis has demonstrated the importance of the assessment of physical literacy with younger children and provides the foundation for the development of a future physical literacy assessment tool for this context, which could have positive impact across research, policy and practice.
A New Tooth Wear–Based Dietary Analysis Method for Proboscidea (Mammalia)
Creators: name: Saarinen, Juha
name: Aleksis Karme
name: Cerling, Thure
name: Uno, Kevin
name: Säilä, Laura
name: Kasiki, Samuel
name: Shadrack Ngene
name: Thadeus Obari
name: Mbua, Emma
name: Manthi, Fredrick Kyalo
name: Fortelius, Mikael
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Biological Sciences
subject: Ecology
subject: FOS: Biological sciences
subject: Chemistry
subject: Earth and Environmental Sciences

description: ABSTRACTDietary analyses of herbivorous mammals are important for paleoecological reconstruction. Several methods applicable to fossil teeth have been developed lately. The mesowear method based on wear-induced occlusal shape and relief of ungulate molars has proven to be a robust method for dietary analysis. In its original form it can only be used for selenodont, plagiolophodont, and ectolophodont ungulate molars, but the principle can be extended to other kinds of tooth morphology. We introduce a new method of dietary analysis for proboscideans similar to the mesowear method, based on angle measurements from worn dentin valleys reflecting the relief of enamel ridges. The enamel ridges should be heavily worn when the abrasiveness of diet increases, resulting in lower occlusal relief and larger angles. For testing this, we compared the mesowear angles with stable carbon isotope values from dental enamel from populations of extant and fossil species from localities from Kenya and India. This enables us to compare diet and tooth wear in proboscideans, because the stable carbon isotope ratios in tropical environments provide a reliable standard for assessing the relative amounts of C4 and C3 plants in diet, and most of the C4 plants are grasses, which should be reflected in the mesowear signal.SUPPLEMENTAL DATA—Supplemental materials are available for this article for free at www.tandfonline.com/UJVP
ABO phenotype protected reproduction based on specific fucosylations and explained by Bombay type formation.
Creators: name: Arend, Peter
Publisher: name: figshare
Year: 2017
Subjects: subject: Hematology
subject: Medicine
subject: Biochemistry
subject: Cancer
subject: Biological Techniques
subject: Immunology
subject: FOS: Clinical medicine
subject: Computational Chemistry
subject: Molecular Biology
subject: Stem Cells
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Anatomy

description: The molecular biological relations between the formation of the ABO(H) blood group phenotype and human fertility is evident in the rare (Oh) or Bombay blood type, which Charles Darwin would have interpreted as resulting from reduced male fertility in consanguinities, based on the history of his own family, the Darwin/Wedgewood Dynasty. In its native form, the Bombay type occurs in individuals with the extremely rare but human specific genotype (h/h; se/se), which does not produce the fucosyltransferases FUT1and FUT2 encoded on chromosome 19 due to point mutations. In fact, the Bombay type individual cannot synthesize the fucosyl residues or H receptor, which in epistatic connection with the A- and B-allelic glycotransferases, encoded on chromosome 9, provides the structural basis for the formation of ABO(H) phenotypes on the cell surfaces, secretions and plasma proteins, such as van Willebrand factor (vWF), clotting factor VIII and the α2-macroglobulin (A2M). The innate isoagglutinins instead show extremely high levels of a mercaptoethanol-sensitive, complement-binding anti-H reactivity, which acts over a wide range of temperatures, with an amplitude at 370C and is thought to reflect a genetically identical non-fucosylation of plasma proteins. Indeed, the FUT1/FUT2-based ABO(H) plasma reactivity, expressed by A2M, which synergistically works with the structurally related, non-immune immunoglobulin M (IgM) molecule, is markedly depleted in Bombay type plasma. Thus, in view of antibody-dependent cellular cytotoxicity (ADCC), exerted by poorly or non-fucosylated immunoglobulins, the unsual anti-H reactivity may signify an autoimmune process, which is primarily based on anti-self-reactive ADCC. While the role of IgM in ADCC is discussed contradictory, the unusual temperature amplitude at 370C of the anti-H-reactivity in Bombay type plasma suggests the function of genetically as yet undefined IgG maintaining and a complex anti-self-reactivity in male gamete performances.
A Sustainable Synthesis of the Naturally Hypolipidemic Agent α-asarone
Creators: name: Camacho-Dávila, Alejandro A.
name: Chávez-Flores, David
name: Zaragoza-Galán, Gerardo
name: Ramos-Sánchez, Víctor H.
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Science Policy
subject: Biological Sciences
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Evolutionary Biology
subject: FOS: Biological sciences
subject: Biotechnology
subject: Pharmacology
subject: Molecular Biology
subject: Cell Biology
subject: Biochemistry

description: :A short and practical preparation of α-asarone was developed using the inexpensive methylisoeugenol as a starting material. The utilization of a sequence of tribromination, debromination and copper-mediated aromatic substitution enabled the stereoselective formation of only the E isomer of α-asarone in good yield.
ABO phenotype protected reproduction based on specific fucosylations and explained by Bombay type formation.
Creators: name: Arend, Peter
Publisher: name: figshare
Year: 2017
Subjects: subject: Hematology
subject: Medicine
subject: Biochemistry
subject: Cancer
subject: Biological Techniques
subject: Immunology
subject: FOS: Clinical medicine
subject: Computational Chemistry
subject: Molecular Biology
subject: Stem Cells
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Anatomy

description: The molecular biological relations between the formation of the ABO(H) blood group phenotype and human fertility is evident in the rare (Oh) or Bombay blood type, which Charles Darwin would have interpreted as resulting from reduced male fertility in consanguinities, based on the history of his own family, the Darwin/Wedgewood Dynasty. In its native form, the Bombay type occurs in individuals with the extremely rare but human specific genotype (h/h; se/se), which does not produce the fucosyltransferases FUT1and FUT2 encoded on chromosome 19 due to point mutations. In fact, the Bombay type individual cannot synthesize the fucosyl residues or H receptor, which in epistatic connection with the A- and B-allelic glycotransferases, encoded on chromosome 9, provides the structural basis for the formation of ABO(H) phenotypes on the cell surfaces, secretions and plasma proteins, such as van Willebrand factor (vWF), clotting factor VIII and the α2-macroglobulin (A2M). The innate isoagglutinins instead show extremely high levels of a mercaptoethanol-sensitive, complement-binding anti-H reactivity, which acts over a wide range of temperatures, with an amplitude at 370C and is thought to reflect a genetically identical non-fucosylation of plasma proteins. Indeed, the FUT1/FUT2-based ABO(H) plasma reactivity, expressed by A2M, which synergistically works with the structurally related, non-immune immunoglobulin M (IgM) molecule, is markedly depleted in Bombay type plasma. Thus, in view of antibody-dependent cellular cytotoxicity (ADCC), exerted by poorly or non-fucosylated immunoglobulins, the unsual anti-H reactivity may signify an autoimmune process, which is primarily based on anti-self-reactive ADCC. While the role of IgM in ADCC is discussed contradictory, the unusual temperature amplitude at 370C of the anti-H-reactivity in Bombay type plasma suggests the function of genetically as yet undefined IgG and a complex anti-self-reactivity in male gamete performances.
title: How Eight Institutions Have Incorporated Appreciative Advising
Creators: name: Bloom, Jennifer L.
name: Hutson, Bryant
name: He, Ye
name: Amundsen, Scott
name: Buyarski, Cathy
name: Christman, Philip
name: Cuevas, Amanda
name: Woodward, L. Kaye
name: Murray, Joseph
name: Robinson, Claire
name: Kucharczyk, Loretta
Publisher: name: The Pennsylvania State University Libraries
Year: 2009

description: The Mentor, Volume 11
A new tooth wear-based dietary analysis method for Proboscidea (Mammalia)
Creators: name: Saarinen, Juha
name: Aleksis Karme
name: Cerling, Thure
name: Uno, Kevin
name: Säilä, Laura
name: Kasiki, Samuel
name: Shadrack Ngene
name: Thadeus Obari
name: Mbua, Emma
name: Manthi, Fredrick Kyalo
name: Fortelius, Mikael
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Biological Sciences
subject: Ecology
subject: FOS: Biological sciences
subject: Chemistry
subject: Earth and Environmental Sciences

description: ABSTRACTDietary analyses of herbivorous mammals are important for paleoecological reconstruction. Several methods applicable to fossil teeth have been developed lately. The mesowear method based on wear-induced occlusal shape and relief of ungulate molars has proven to be a robust method for dietary analysis. In its original form it can only be used for selenodont, plagiolophodont, and ectolophodont ungulate molars, but the principle can be extended to other kinds of tooth morphology. We introduce a new method of dietary analysis for proboscideans similar to the mesowear method, based on angle measurements from worn dentin valleys reflecting the relief of enamel ridges. The enamel ridges should be heavily worn when the abrasiveness of diet increases, resulting in lower occlusal relief and larger angles. For testing this, we compared the mesowear angles with stable carbon isotope values from dental enamel from populations of extant and fossil species from localities from Kenya and India. This enables us to compare diet and tooth wear in proboscideans, because the stable carbon isotope ratios in tropical environments provide a reliable standard for assessing the relative amounts of C4 and C3 plants in diet, and most of the C4 plants are grasses, which should be reflected in the mesowear signal.SUPPLEMENTAL DATA—Supplemental materials are available for this article for free at www.tandfonline.com/UJVP
Automated Library Construction Using KAPA Library Preparation Kits on the Agilent NGS Workstation Yields High-Quality Libraries for Whole-Genome Sequencing on the Illumina Platform
Creators: name: Kong, Nguyet
name: Kao Thao
name: Huang, Carol
name: Storey, Dylan
name: Weimer, Bart
name: Maryke Appel
name: Lappin, Stephen
name: Knapp, Lisa
name: Kelly, Lenore
Publisher: name: figshare
Year: 2015
Subjects: subject: Molecular Biology
subject: Microbiology
subject: FOS: Biological sciences

description: A new method was developed to automate the KAPA HTP Library Preparation kit for microbial whole genome sequencing. This method uses the Agilent NGS Workstation, consisting of the NGS Bravo liquid handling platform with its acces- sories for heating, cooling, shaking, and magnetic bead manipulations in a 96-well of format. User intervention in multistep protocols is minimized through the use of other components of the workstation such as the BenchCel 4R Microplate Handler and Labware MiniHub for labware storage and movement. This method has been validated for sequencing on the Illumina platform and consists of three protocols: the first is for end repair to post-ligation cleanup; the second is used for library amplification setup; and the third is for the post-amplification cleanup. The modular design provides the end-user with the flexibility to complete library construction over two days, and is suitable for the construction of high-quality libraries from bac- teria of various GC content. This combined solution produced a workflow that is suitable for production-scale sequencing projects such as the 100K Pathogen Genome Project.
Letter to the Medical Community: Emergent findings on Autonomic Dysfunctions in H63D Syndrome across all three types of the disease, including Oshtoran Syndrome
Creators: name: Diamandis, Carolina
name: Panaitescu, Roxana
name: Consortium, H63D Research
name: Strelkov, Boris
name: Rocha, Fabio
Publisher: name: figshare
Year: 2024
Subjects: classificationCode: 429999
subject: Other health sciences not elsewhere classified

description: Recent investigations have unveiled pivotal findings concerning autonomic dysfunctions within the H63D syndrome spectrum. While it is well-documented that Oshtoran Syndrome (H63D Syndrome Type 3) is characterized by life-threatening autonomic dysfunctions impacting organ systems, the endocrine system, and the immune system through innervation, our latest research reveals that such dysfunctions are not confined to Type 3 alone. Our data suggest that Types 1 and 2 of the H63D syndrome also exhibit autonomic dysfunctions, albeit in a milder form.
Quality Control of High-Throughput Library Construction Pipeline for KAPA HTP Library Using an Agilent 2200 TapeStation
Creators: name: Kong, Nguyet
name: Ng, Whitney
name: Azarene Foutouhi
name: Huang, Carol
name: Storey, Dylan
name: Weimer, Bart
name: Kelly, Lenore
Publisher: name: figshare
Year: 2015
Subjects: Molecular Biology

description: Next Generation Sequencing requires the input of high molecular weight genomic DNA to construct quality libraries for whole genome bacterial sequencing. Large scale sequencing projects, such as the 100K Pathogen Genome Project, require
100K Pathogen Genome Project methods to rapidly assess the quantity and quality of the input DNA using high-throughput methods that are fast and cost effective. In this study, the Agilent 2200 TapeStation and Agilent 2100 Bioanalyzer Systems were used to assess a few critical quality control steps for library construction. With minimal manual interven-tion, the Agilent 2200 TapeStation System determined the quality of genomic DNA, fragmented DNA, and final libraries constructed from multiple types of foodborne pathogens. The Agilent 2200 TapeStation System provided a single platform that effectively evaluated the necessary quality control steps, which provided a distinct advantage to decrease the time needed for library construction and a common
instrument methodology for quality control.
Synthesis, structural, and ε-caprolactone polymerization studies of heteroleptic derivatives of aluminum(III)
Creators: name: Vuppalapati Giri Prasanth
name: Tummalapalli Kiran
name: Paduthapillai Gopal Aravindan
name: Kulathu Iyer Sathiyanarayanan
name: Madhvesh Pathak
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: 60506 Virology
subject: FOS: Biological sciences
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Cell Biology
subject: Microbiology
subject: Biochemistry

description: A series of metallo-organic complexes of Al(III), [(CH3COCHCOCH3)2Al(OR)]2 [where R = o-MeC6H4 (1), m-MeC6H4 (2), p-MeC6H4 (3), C6H5CH2 (4), o-ClC6H4CH2 (5), m-ClC6H4CH2 (6), and p-ClC6H4CH2 (7), were synthesized quantitatively by treating Al(OPri)3 with CH3COCH2COCH3 and the phenols (o-MeC6H4OH, m-MeC6H4OH, and p-MeC6H4OH) concerned as well as phenylmethanols (C6H5CH2OH, o-ClC6H4CH2OH, m-ClC6H4CH2OH, and p-ClC6H4CH2OH) in suitable stoichiometry using benzene as solvent. All these complexes were soluble in common organic solvents, having sharp melting points, and they were characterized by elemental analysis, IR, and NMR (1H and 13C) spectral studies. Single-crystal XRD followed by ORTEP diagram of solvated complex (1a) revealed its dimeric nature and the existence of six-coordinate aluminum. The complexes were studied in ring-opening polymerization of ε-caprolactone. The molecular weight and polydispersity index values of polycaprolactone were obtained by gel permeation chromatography analysis.
Environmental influences on movements and distribution of a wild horse (Equus caballus) population in western Nevada, USA: a 25-year study
Creators: name: Turner, John W.
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: 111714 Mental Health
subject: Biological Sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: Ecology
subject: FOS: Biological sciences
subject: Earth and Environmental Sciences
subject: Evolutionary Biology
subject: Neuroscience

description: Wild horse populations in the American West have been heavily managed to regulate horse numbers since the inception of the Free-Roaming Wild Horse and Burro Act of 1971. The Montgomery Pass Wild Horse Territory (MPWHT) on the California/Nevada border is unique in the absence of human intervention there across the past 30 years. This has provided the opportunity to observe long-term patterns in the natural relationship between wild horses and their environment and to examine environmental impacts on the horse population and its activities, movements and distribution on the range. In this study multiple variables in the physical environment and in horse behaviour were monitored seasonally across 25 years beginning in 1987. Distinct summer (higher elevations) and winter (lower elevations) range use was characteristic for more than 60% of the population during the first 7 study years, with subsequent gradual but marked reduction in use of summer range. While approximately 20% of the population continued to annually use the historical summer range, the majority divided into two geographically and functionally separate subpopulations that resided year round in the historical winter range and adjacent areas on opposite sides of the MPWHT. Mountain lion predation on foals was restricted to the summer range, and exodus of horses from the summer range resulted in increased foal survival where horse subpopulations eventually resided. The long-term consequences of increased horse numbers in the MPWHT remain under continued study. The present study has shown that wild horses are highly adaptive and individually varied in response to environmental pressures. It has also demonstrated the value of long-term monitoring of wild horse populations to reveal underlying dynamics and their potential management implications.
ABO phenotype protected reproduction based on specific fucosylations and explained by Bombay type formation.
Creators: name: Arend, Peter
Publisher: name: figshare
Year: 2017
Subjects: subject: Hematology
subject: Medicine
subject: Biochemistry
subject: Cancer
subject: Biological Techniques
subject: Immunology
subject: FOS: Clinical medicine
subject: Computational Chemistry
subject: Molecular Biology
subject: Stem Cells
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Anatomy

description: The molecular biological relations between the formation of the ABO(H) blood group phenotype and human fertility is evident in the rare (Oh) or Bombay blood type, which Charles Darwin would have interpreted as resulting from reduced male fertility in consanguinities, based on the history of his own family, the Darwin/Wedgewood Dynasty. In its native form, the Bombay type occurs in individuals with the extremely rare but human specific genotype (h/h; se/se), which does not produce the fucosyltransferases FUT1and FUT2 encoded on chromosome 19 due to point mutations. In fact, the Bombay type individual cannot synthesize the fucosyl residues or H receptor, which in epistatic connection with the A- and B-allelic glycotransferases, encoded on chromosome 9, provides the structural basis for the formation of ABO(H) phenotypes on the cell surfaces, secretions and plasma proteins, such as van Willebrand factor (vWF), clotting factor VIII and the α2-macroglobulin (A2M). The innate isoagglutinins instead show extremely high levels of a mercaptoethanol-sensitive, complement-binding anti-H reactivity, which acts over a wide range of temperatures, with an amplitude at 370C and is thought to reflect a genetically identical non-fucosylation of plasma proteins. Indeed, the FUT1/FUT2-based ABO(H) plasma reactivity, expressed by A2M, which synergistically works with the structurally related, non-immune immunoglobulin M (IgM) molecule, is markedly depleted in Bombay type plasma. Thus, in view of antibody-dependent cellular cytotoxicity (ADCC), exerted by poorly or non-fucosylated immunoglobulins, the unsual anti-H reactivity may signify an autoimmune process, which is primarily based on anti-self-reactive ADCC. However, while the role of IgM in ADCC is discussed contradictory, the temperature amplitude at 370C may suggest the associated functions of genetically as yet undefined IgG and a complex anti-self-reactivity affecting male gamete performance.
Synthesis, structural and ε-caprolactone polymerization studies of heteroleptic derivatives of aluminium(III)
Creators: name: Vuppalapati Giri Prasanth
name: Tummalapalli Kiran
name: Paduthapillai Gopal Aravindan
name: Kulathu Iyer Sathiyanarayanan
name: Madhvesh Pathak
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: 60506 Virology
subject: FOS: Biological sciences
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Cell Biology
subject: Microbiology
subject: Biochemistry

description: A series of metallo-organic complexes of Al(III), [(CH3COCHCOCH3)2Al(OR)]2 [where R = o-MeC6H4 (1), m-MeC6H4 (2), p-MeC6H4 (3), C6H5CH2 (4), o-ClC6H4CH2 (5), m-ClC6H4CH2 (6) and p-ClC6H4CH2 (7), were synthesized quantitatively by treating Al(OPri)3 with CH3COCH2COCH3 and the phenols (o-MeC6H4OH, m-MeC6H4OH and p-MeC6H4OH) concerned as well as phenylmethanols (C6H5CH2OH, o-ClC6H4CH2OH, m-ClC6H4CH2OH and p-ClC6H4CH2OH) in suitable stoichiometry using benzene as solvent. All these complexes were soluble in common organic solvents, having sharp melting points, and they were characterized by elemental analysis, IR and NMR (1H and 13C) spectral studies. Single crystal XRD followed by ORTEP diagram of solvated complex (1a) revealed its dimeric nature and the existence of six-coordinate aluminium. The complexes were studied in ring opening polymerization of ε-caprolactone. The molecular weight and polydispersity index values of polycaprolactone were obtained by GPC analysis.
Breast Cancer Cells and PD-1/PD-L1 Blockade Upregulate the Expression of PD-1, CTLA-4, TIM-3 and LAG-3 Immune Checkpoints in CD4+ T Cells
Creators: name: Saleh, Reem
name: M Toor, Salman
name: Khalaf, Sarah
name: Elkord, Eyad
Publisher: name: Manara - Qatar Research Repository
Year: 2024
Subjects: classificationCode: 32
subject: Biomedical and clinical sciences
classificationCode: 3211
subject: Oncology and carcinogenesis

description: Triple negative breast cancer (TNBC) is the most aggressive breast cancer subtype, and it exhibits resistance to common breast cancer therapies. Immune checkpoint inhibitors (ICIs) targeting programmed cell death 1 (PD-1) and its ligand, PD-L1, have been approved to treat various cancers. However, the therapeutic efficacy of targeting PD-1/PD-L1 axis in breast cancer is under clinical investigation. In addition, the mechanisms of action of drugs targeting PD-1 and PD-L1 have not been fully elucidated. In this study, we investigated the effect of human TNBC cell lines, MDA-MB-231 and MDA-MB-468, and the non-TNBC cell line, MCF-7, on the expression of immune checkpoints (ICs) on CD4+ T cell subsets, including regulatory T cells (Tregs), using a co-culture system. We also examined the effect of blocking PD-1 or PD-L1 separately and in combination on IC expression by CD4+ T cell subsets. We found that breast cancer cells upregulate the expression of ICs including PD-1, cytotoxic T lymphocyte-associated antigen-4 (CTLA-4), T cell immunoglobulin and mucin domain-containing protein 3 (TIM-3) and lymphocyte activation gene-3 (LAG-3) in CD4+ T cell subsets. We also found that the co-blockade of PD-1 and PD-L1 further upregulates the co-expression of TIM-3 and LAG-3 on CD4+CD25+ T cells and CD4+CD25+FoxP3+Helios+ Tregs in the presence of TNBC cells, but not in non-TNBC cells. Our results indicate the emergence of compensatory inhibitory mechanisms, most likely mediated by Tregs and activated non-Tregs, which could lead to the development of TNBC resistance against PD-1/PD-L1 blockade. Other InformationPublished in: Vaccines
License: https://creativecommons.org/licenses/by/4.0/
See article on publisher's website: https://doi.org/10.3390/vaccines7040149
Yield Curve Construction
Creators: name: Yang, Alex
Publisher: name: Zenodo
Year: 2020
Subjects: Yield curve, zero curve, curve construction, curve bootstrapping

description: Yield curve is defined as the relationship between the yield-to-maturity on a zero coupon bond and the bond’s maturity. Zero yield curves play an essential role in financial valuation as one needs t forecasting future cashflows using appropriate forward rates and discounting the flows.
description: https://ia601505.us.archive.org/29/items/ir-curve-2/IrCurve-2.pdf
Characterising riparian buffer zones of an agriculturally modified landscape
Creators: name: K Renouf
name: JS Harding
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Plant Biology
subject: FOS: Biological sciences
subject: Biological Sciences
subject: Ecology
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Cell Biology
subject: Biochemistry
subject: Biophysics

description: Riparian buffer zones are viewed as an important management tool for waterway protection. However, little qualitative or quantitative assessment has been made of their dimensions or composition. We surveyed 88 small agricultural waterways within the Canterbury region to characterise the width and vegetative composition of riparian ‘buffer’ zones. Less than 20% of buffer zones were 10 m or wider and approximately 65% ≤ 5 m wide. Mean plant diversity was lowest in buffers ≤ 5 m (four taxa) and highest in buffers 25–30 m wide (11 taxa), although 98% of all taxa recorded in the survey occurred within 5 m of waterways. Exotic pasture grasses, weeds and adventive trees and shrubs dominated riparian areas at all distances from the -side. Furthermore, buffer width and vegetation type did not differ with adjacent land use despite regional efforts to plant native vegetation and regulatory emphasis on reducing water contamination from intensifying land use practices.
Heterologous production of l-lysine ε-oxidase by directed evolution using a fusion reporter method
Creators: name: Matsui, Daisuke
name: Asano, Yasuhisa
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: 60506 Virology
subject: FOS: Biological sciences
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: Information and Computing Sciences
subject: Biological Sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: Meta Science
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Evolutionary Biology
subject: Molecular Biology
subject: Genetics
subject: Microbiology
subject: Biochemistry

description: For the heterologous production of l-lysine ε-oxidase (LodA), we constructed a new plasmid carrying LodA gene fused in-frame with an antibiotic (phleomycine) resistant gene. The new plasmid was randomly mutated and the mutated plasmids were transformed into Escherichia coli BL21 (DE3) harboring lodB, which encodes a protein (LodB) acting in posttranslational modification of LodA, and active mutants were selected by phleomycin resistance and oxidase activities. One soluble LodA variant isolated by this method contained six silent mutations and one missense mutation. At these mutation points, the codon adaptations at Lys92, Ala550, and Thr646, and the amino acid substitution at His286 to Arg contributed to the production of its functional form. The active form of LodA variant was induced by post-modification of LodB in the heterologous coexpression, and the activity increased with additional NaCl and heat treatment. This is the first report of heterologous production of LodA by random mutagenesis.