title: ÐÐ·ÑÑÐµÐ½Ð¸Ðµ ÑÑÑÑÐºÑÑÑÑ ÐºÐ°ÑÐ¸Ð¾ÑÐ¸Ð¿Ð° ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ð°Ð»ÑÐ½ÑÑ Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð¿Ð¾ÑÐ»Ðµ ÐºÑÐ°ÑÐºÐ¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð²Ð¾Ð·Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ ÑÐµÐ½ÑÐ³ÐµÐ½Ð¾Ð²ÑÐºÐ¾Ð³Ð¾ Ð¾Ð±Ð»ÑÑÐµÐ½Ð¸Ñ
title: Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÑÐºÐ°Ñ ÑÐ°Ð±Ð¾ÑÐ°
titleType: Subtitle
Creators: name: ÐÐ¾Ð¿ÐµÐ»ÑÑÐºÐ¾, Ð®Ð»Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2016
Subjects: ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ð°Ð»ÑÐ½ÑÐµ Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÐµ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸
Ð´ÐµÑÐºÐ²Ð°Ð¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¹ ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ð¹
ÐºÐ°ÑÐ¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ð¹ Ð°Ð½Ð°Ð»Ð¸Ð·
ÐºÐ°ÑÐ¸Ð¾ÑÐ¸Ð¿Ð¸ÑÐµÑÐºÐ°Ñ ÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾ÑÑÑ
ÑÐµÐ½ÑÐ³ÐµÐ½Ð¾Ð²ÑÐºÐ¾Ðµ Ð¸Ð·Ð»ÑÑÐµÐ½Ð¸Ðµ
endometrial mesenchymal stem cells
desquamated endometrium
karyological analysis
karyotype stability
X-rays

description: Ð­Ð½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ð°Ð»ÑÐ½ÑÐµ Ð¼ÐµÐ·ÐµÐ½ÑÐ¸Ð¼Ð½ÑÐµ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸ (ÑÐÐ¡Ð) ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð² Ð½Ð°ÑÑÐ¾ÑÑÐµÐµ Ð²ÑÐµÐ¼Ñ ÑÐ°ÑÑÐ¼Ð°ÑÑÐ¸Ð²Ð°ÑÑÑÑ ÐºÐ°Ðº Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»ÑÐ½ÑÐ¹ Ð¸ÑÑÐ¾ÑÐ½Ð¸Ðº ÑÑÐ°Ð½ÑÐ¿Ð»Ð°Ð½ÑÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð³Ð¾ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ð° Ð´Ð»Ñ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐµÑÐ°Ð¿Ð¸Ð¸. ÐÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð´Ð°Ð½Ð½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð² Ð¼ÐµÐ´Ð¸ÑÐ¸Ð½ÑÐºÐ¸Ñ ÑÐµÐ»ÑÑ Ð¿Ð¾Ð´ÑÐ°Ð·ÑÐ¼ÐµÐ²Ð°ÐµÑ Ð½Ð°Ð»Ð¸ÑÐ¸Ðµ Ð½Ð¾ÑÐ¼Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÐºÐ°ÑÐ¸Ð¾ÑÐ¸Ð¿Ð°. ÐÐ¾ÑÐºÐ¾Ð»ÑÐºÑ Ð½Ð° ÑÐµÐ³Ð¾Ð´Ð½ÑÑÐ½Ð¸Ð¹ Ð´ÐµÐ½Ñ Ð½ÐµÑ Ð¾Ð´Ð½Ð¾Ð·Ð½Ð°ÑÐ½Ð¾Ð³Ð¾ Ð¾ÑÐ²ÐµÑÐ° Ð¾ ÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾ÑÑÐ¸ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ð° ÑÐÐ¡Ð Ð¿ÑÐ¸ Ð¿ÐµÑÐµÑÐ¾Ð´Ðµ Ð² ÑÐ¸ÑÑÐµÐ¼Ñ in vitro, Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°ÑÑ ÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾ÑÑÑ ÐºÐ°ÑÐ¸Ð¾ÑÐ¸Ð¿Ð° ÑÐÐ¡Ð Ð½Ð° ÐºÐ°Ð¶Ð´Ð¾Ð¼ ÑÑÐ°Ð¿Ðµ ÑÐ°Ð±Ð¾ÑÑ Ñ ÑÑÐ¸Ð¼Ð¸ ÐºÐ»ÐµÑÐºÐ°Ð¼Ð¸. ÐÐ°Ð´Ð°ÑÐµÐ¹ Ð½Ð°ÑÑÐ¾ÑÑÐµÐ³Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¾ Ð¸Ð·ÑÑÐ¸ÑÑ ÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾ÑÑÑ ÑÑÑÑÐºÑÑÑÑ ÐºÐ°ÑÐ¸Ð¾ÑÐ¸Ð¿Ð° ÑÐÐ¡Ð Ð¿Ð¾ÑÐ»Ðµ Ð²Ð¾Ð·Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ ÑÑÐ±Ð»ÐµÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð´Ð¾Ð·Ñ ÑÐµÐ½ÑÐ³ÐµÐ½Ð¾Ð²ÑÐºÐ¾Ð³Ð¾ Ð¸Ð·Ð»ÑÑÐµÐ½Ð¸Ñ. ÐÐ±ÑÐµÐºÑÐ¾Ð¼ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¿Ð¾ÑÐ»ÑÐ¶Ð¸Ð»Ð¸ ÑÐÐ¡Ð ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°, Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½ÑÐµ Ð¸Ð· Ð´ÐµÑÐºÐ²Ð°Ð¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ñ Ð¼ÐµÐ½ÑÑÑÑÐ°Ð»ÑÐ½Ð¾Ð¹ ÐºÑÐ¾Ð²Ð¸. ÐÐ»ÐµÑÐ¾ÑÐ½Ð°Ñ Ð»Ð¸Ð½Ð¸Ñ Ð±ÑÐ»Ð° Ð¿Ð¾Ð»ÑÑÐµÐ½Ð° ÑÐ¾ÑÑÑÐ´Ð½Ð¸ÐºÐ°Ð¼Ð¸ Ð³ÑÑÐ¿Ð¿Ñ ÐÐÐÐ¸ÐÐ ÐÐ½ÑÑÐ¸ÑÑÑÐ° ÑÐ¸ÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð ÐÐ. ÐÐ»Ñ Ð¾ÑÐµÐ½ÐºÐ¸ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ð¹ ÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾ÑÑÐ¸ Ð½Ð° ÑÑÐ¾Ð²Ð½Ðµ ÐºÐ°ÑÐ¸Ð¾ÑÐ¸Ð¿Ð° Ð² ÑÐ°Ð±Ð¾ÑÐµ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ Ð¼ÐµÑÐ¾Ð´ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð¹ Ð¾ÐºÑÐ°ÑÐºÐ¸ Ð¼ÐµÑÐ°ÑÐ°Ð·Ð½ÑÑ ÑÑÐ¾Ð¼Ð¾ÑÐ¾Ð¼ Ð½Ð° G-Ð´Ð¸ÑÐºÐ¸. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ Ð´Ð°Ð½Ð½ÑÐµ Ð¿Ð¾Ð·Ð²Ð¾Ð»Ð¸Ð»Ð¸ Ð³Ð¾Ð²Ð¾ÑÐ¸ÑÑ Ð¾ ÑÐ¾Ð¼, ÑÑÐ¾ Ð²Ð¾Ð·Ð´ÐµÐ¹ÑÑÐ²Ð¸Ðµ ÑÐµÐ½ÑÐ³ÐµÐ½Ð¾Ð²ÑÐºÐ¾Ð³Ð¾ Ð¸Ð·Ð»ÑÑÐµÐ½Ð¸Ñ Ð² ÑÑÐ±Ð»ÐµÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð´Ð¾Ð·Ðµ Ð¿ÑÐ¸Ð²Ð¾Ð´Ð¸Ñ Ðº ÑÑÑÐµÑÑÐ²ÐµÐ½Ð½Ð¾Ð¹ ÐºÐ°ÑÐ¸Ð¾ÑÐ¸Ð¿Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð½ÐµÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾ÑÑÐ¸ ÑÐÐ¡Ð. ÐÑÐ½Ð¾Ð²Ð½ÑÐ¼ ÑÐ¸Ð¿Ð¾Ð¼ Ð¿ÐµÑÐµÑÑÑÐ¾ÐµÐº Ð² Ð¾Ð±Ð»ÑÑÐµÐ½Ð½ÑÑ ÑÐÐ¡Ð Ð±ÑÐ»Ð¸ ÑÑÐ¾Ð¼Ð¾ÑÐ¾Ð¼Ð½ÑÐµ Ð¿Ð¾Ð»Ð¾Ð¼ÐºÐ¸. ÐÐ°Ð¸Ð±Ð¾Ð»ÐµÐµ Ð¿Ð¾Ð´Ð²ÐµÑÐ¶ÐµÐ½Ñ Ð¿Ð¾Ð»Ð¾Ð¼ÐºÐ°Ð¼ Ð¾ÐºÐ°Ð·Ð°Ð»Ð¸ÑÑ ÑÑÐ¾Ð¼Ð¾ÑÐ¾Ð¼Ñ 1,4 Ð¸ Ð¥. Ð¡ÑÐ°Ð²Ð½Ð¸ÑÐµÐ»ÑÐ½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð· Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹ Ð² ÑÑÑÑÐºÑÑÑÐµ ÐºÐ°ÑÐ¸Ð¾ÑÐ¸Ð¿Ð° ÑÐÐ¡Ð, Ð¿Ð¾Ð´Ð²ÐµÑÐ³Ð½ÑÑÑÑ ÑÐ°Ð´Ð¸Ð°ÑÐ¸Ð¸, Ñ ÐºÐ°ÑÐ¸Ð¾ÑÐ¸Ð¿Ð¸ÑÐµÑÐºÐ¸Ð¼Ð¸ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸ÑÐ¼Ð¸ Ð² Ð´Ð°Ð½Ð½ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ, Ð¿ÑÐµÑÐµÑÐ¿ÐµÐ²ÑÐ¸Ñ ÑÐµÑÐ¼Ð¾ÑÐ¾Ðº, Ð²ÑÑÐ²Ð¸Ð» Ð¾Ð´Ð½Ð¾ÑÐ¸Ð¿Ð½Ð¾ÑÑÑ ÑÐ¸ÑÐ¾Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹. ÐÐ°Ð¸Ð±Ð¾Ð»ÐµÐµ Ð²Ð¾Ð²Ð»ÐµÑÐµÐ½Ð½ÑÐµ Ð² Ð¿Ð¾Ð»Ð¾Ð¼ÐºÐ¸ Ð¿ÑÐ¸ Ð´Ð²ÑÑ ÑÐ¸Ð¿Ð°Ñ ÑÑÑÐµÑÑÐ° Ð¾ÐºÐ°Ð·Ð°Ð»Ð¸ÑÑ 1-Ð°Ñ Ð¸ 4-Ð°Ñ ÑÑÐ¾Ð¼Ð¾ÑÐ¾Ð¼Ñ.
description: Endometrial mesenchymal stem cells (EMSC) human being considered as a potential source of transplant material for cell therapy. The use of these cells for medical purposes implies a normal karyotype. Because today there is no single answer to the stability of the genetic material in the transition EMSC in an in vitro system, it is necessary to analyze the stability of the karyotype EMSC at each stage of the work with these cells. The aim of the present study was to investigate the stability of the karyotype structure of the EMSC after exposure to sublethal doses of X-rays. The object of study is based on the EMSC person isolated from desquamated endometrium menstrual blood. The cell line was derived by employees group GMDiMK Institute of Cytology RAS. To assess the genetic stability of the karyotype at work we used the method of differential staining of chromosomes on the metaphase G-wheels.The data allowed to talk about what the impact of X-rays in sublethal dose leads to a substantial karyotypic instability, the EMSC. The main type of rearrangements in irradiated EMSC were chromosome breakage. The most susceptible to breakages were chromosomes 1,4 and X.A comparative analysis of the changes in the structure of the karyotype EMSC exposed to radiation from the karyotypic changes in the cells which have undergone thermal shock, found the same type of cytogenetic changes. Most involved in the breakage stress when two types were the first and the fourth chromosome.