Metadata supporting the published article: Cyclin E expression is associated with high levels of replication stress in triple-negative breast cancer.
Creators: name: Llobet, Sergi Guerrero
name: Vegt, Bert Van Der
name: Jongeneel, Evelien
name: Bense, Rico D.
name: Zwager, Mieke C.
name: Schröder, Carolien P.
name: Everts, Marieke
name: Fehrmann, Rudolf S.N.
name: Bock, Geertruida H. De
name: Vugt, Marcel A.T.M. Van
Publisher: name: figshare
Year: 2020
Subjects: subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Biological Techniques
subject: Cancer
subject: 111203 Cancer Genetics
subject: FOS: Clinical medicine
subject: FOS: Clinical medicine
subject: 111201 Cancer Cell Biology
subject: Biomarkers
subject: 111209 Solid Tumours

description: Replication stress entails the improper progression of DNA replication. In cancer cells, including breast cancer cells, an important cause of replication stress is oncogene activation. To uncover the relation between oncogene expression, replication stress and clinical features of breast cancer subgroups, the authors immunohistochemically analyzed the expression of a panel of oncogenes (Cyclin E, c-Myc, Cdc25A,) and markers of replication stress (phospho-Ser33-RPA32 and γ-H2AX) in breast tumor tissues prior to treatment.<br><b>Data access:</b> The datasets generated and analysed during the current are publicly available in Mendeley Data: <b>https://doi.org/10.17632/4pnhd8d7rw.1</b>. Uncropped blots from figure 1, are available as part of the supplementary information files (Supplementary Figures 5).<br><b>Patient consent and study approval</b>: Tissue collection and storage of clinicopathological and follow up data was only performed upon patients’ approval via informed consent. Clinicopathological data gathered in this study were anonymized, and used in accordance with the Declaration of Helsinki, and in line with the regulations posed by the Institutional Review Board (IRB) of the University Medical Center Groningen (UMCG).<br><b>Study aims and methodology</b>: Genomically instable tumors increasingly rely for their survival on mechanisms that allow cells to resolve replication stress-induced DNA lesions, including cell cycle checkpoints. In order to implement novel therapeutic agents that target tumors with high levels of replication stress optimally, it is essential to know which tumor subgroups display replication stress. With the aim of finding potential biomarkers for tumors with high levels of replication stress, the authors examined replication stress levels in relation to oncogene expression and clinicopathological data in a consecutive well-defined series of breast cancer samples.The following cell line was used: The triple negative breast cancer cell line MDA-MB-231.Immunohistochemical analysis was performed on tissue specimens taken prior to treatment of 558 patients with breast cancer who underwent surgery at the UMCG.The following procedures are described in more detail in the published article: DNA cloning and retroviral transduction, Western blotting, DNA fiber analysis, MTT assays, immunohistochemistry, evaluation of mRNA expression of CCNE1, MYC and CDC25A, and statistical analysis.<br><b>Datasets supporting the figures, tables, and supplementary tables:</b> The file <b>Llobet et al.xlsx</b> contains the dateset names and file formats of all the datasets supporting the figures and tables in the published article.<br><b>Software needed to access data:</b> Files in. pzfx file format require the GraphPad Prism software to be accessed. Files in. sav and .sps file format require the SPSS statistics software to be accessed.<br><br>