Additional file 1 of Peroxisome proliferator-activated receptor gamma gene variants modify human airway and systemic responses to indoor dibutyl phthalate exposure
Creators: name: Leung, Clarus
name: Ryu, Min Hyung
name: Bølling, Anette Kocbach
name: Maestre-Batlle, Danay
name: Rider, Christopher F.
name: Hüls, Anke
name: Urtatiz, Oscar
name: MacIsaac, Julie L.
name: Lau, Kevin Soon-Keen
name: Lin, David Tse Shen
name: Kobor, Michael S.
name: Carlsten, Chris
Publisher: name: figshare
Year: 2022
Subjects: subject: Immunology
subject: FOS: Clinical medicine

description: Additional file 1: Table S1. DNA sequences for PPARG used to perform targeted pyrosequencing. Table S2. The genotypes and GRS are illustrated for rs709158, rs3856806, and rs10865710. Table S3. The weights of each SNP in the construction of P-GRS. Table S4. Oxidative stress-related genes included in the associated OS-GRS and definition of their risk alleles. Table S5. Exposure-by-P-GRS interaction on DBP effects on (A) blood immune cells at 3h, (B) blood immune cells at 20h, and (C) BAL immune cells at 24h. Table S6. Participant characteristics and oxidative stress genetic risk score (OS-GRS). Figure S1. Effect of OS-GRS interaction on DBP effect on IgE and Th2 lymphocytes. Blood allergen-specific IgE at 3 and 20 h (A-B), and BAL allergen-specific IgE at 24 h (C); blood % Th2 lymphocytes at 3 and 20 h (D-E), and BAL % Th2 at 24 h (F) after the allergen challenge post-DBP. The histogram underlying the plot illustrates the distribution of the participants’ OS-GRS. Blood and BAL samples were collected for 16 and 11 participants, respectively. The histogram underlying each plot illustrates the distribution of the participants’ OS-GRS. Effect values represent the DBP-attributable difference between the measurement at the given time point and the baseline. The solid line represents the interaction effect, with the p value corresponding to the interaction significance. The shaded region represents 95% confidence intervals. Figure S2. Exposure-by-OS-GRS interaction on DBP effect on lung function outcomes. Lung function outcomes included: (A) FEV1 (% predicted), ∆20 h; (B) Methacholine challenge (LogPC20), 20 h; (C) FeNO, ∆20 h; (D) Allergen AUC, 3 h. The listed time points refer to the time after the allergen challenge post-DBP or CA exposure. FEV1 = forced expiratory volume in one second; PC20 = concentration leading to 20% fall in FEV1; FeNO = fractional exhaled nitric oxide; AUC = area under curve, measured as percent decline in FEV1 spanning baseline to 3 h after allergen challenge. The histogram underlying each plot illustrates the distribution of the participants’ OS-GRS. Effect values represent the DBP-attributable difference between the measurement at the given time point and the baseline. The solid line represents the interaction effect, with the p value corresponding to the interaction significance. The shaded region represents 95% confidence intervals. Figure S3. PPAR-γ single nucleotide polymorphism rs10865710. Effect modification by genotype G/C or C/C on (A) Blood IgE at 3 and 20 hours post-exposure to CA with allergen challenge (Air+Ag) or DBP with allergen challenge (DBP+Ag), (B) Blood Th2 lymphocytes at 3 and 20 hours post-exposure to CA (Air) or DBP, and (C) BAL Th2 lymphocytes at 24 hours post-exposure to CA (Air) or DBP.