Urokinase receptor counteracts vascular smooth muscle cell functional changes induced by surface topography
Creators: name: Kiyan, Yulia
name: Kurselis, Kestutis
name: Kiyan, Roman
name: Haller, Hermann
name: Chichkov, Boris
name: Dumler, Inna
Publisher: name: Wyoming, NSW : Ivyspring
Year: 2013
Subjects: subject: Focal adhesion
subject: Microstructured biomaterial
subject: Urokinase receptor
subject: Vascular injury
subject: Vascular smooth muscle cell
subject: connexin 43
subject: focal adhesion kinase
subject: polymer
subject: smooth muscle actin
subject: urokinase receptor
subject: article
subject: cell adhesion
subject: cell communication
subject: cell elongation
subject: cell function
subject: cell proliferation
subject: cell shape
subject: cell structure
subject: cell transdifferentiation
subject: cellular distribution
subject: controlled study
subject: cytoskeleton
subject: human
subject: human cell
subject: phenotype
subject: protein expression
subject: protein function
subject: protein localization
subject: smooth muscle fiber
subject: spindle cell
subject: upregulation
subject: vascular smooth muscle
subject: Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit

description: Molecular beacons (MBs) of DCurrent treatments for human coronary artery disease necessitate the development of the next generations of vascular bioimplants. Recent reports provide evidence that controlling cell orientation and morphology through topographical patterning might be beneficial for bioimplants and tissue engineering scaffolds. However, a concise understanding of cellular events underlying cell-biomaterial interaction remains missing. In this study, applying methods of laser material processing, we aimed to obtain useful markers to guide in the choice of better vascular biomaterials. Our data show that topographically treated human primary vascular smooth muscle cells (VSMC) have a distinct differentiation profile. In particular, cultivation of VSMC on the microgrooved biocompatible polymer E-shell induces VSMC modulation from synthetic to contractile phenotype and directs formation and maintaining of cell-cell communication and adhesion structures. We show that the urokinase receptor (uPAR) interferes with VSMC behavior on microstructured surfaces and serves as a critical regulator of VSMC functional fate. Our findings suggest that microtopography of the E-shell polymer could be important in determining VSMC phenotype and cytoskeleton organization. They further suggest uPAR as a useful target in the development of predictive models for clinical VSMC phenotyping on functional advanced biomaterials.NA and RNA have aroused increasing interest because they allow a continuous readout, excellent spatial and temporal resolution to observe in real time. This kind of dual-labeled oligonucleotide probes can differentiate between bound and unbound DNA/RNA in homogenous hybridization with a high signal-to-background ratio in living cells. This review briefly summarizes the different unnatural sugar backbones of oligonucleotides combined with fluoro-phores that have been employed to sense DNA/RNA. With different probes, we epitomize the fundamental understanding of driving forces and these recognition processes. Moreover, we will introduce a few novel and attractive emerging applications and discuss their advantages and dis-advantages. We also highlight several perspective probes in the application of cancer therapeutics.