title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ Ð°Ð½ÑÐ¸Ð±Ð¸Ð¾ÑÐ¸ÐºÐ° ÑÐµÑÐ¼Ð¾ÑÑÐ±Ð¸Ð½Ð° Ð½Ð° Ð¿Ð°ÑÑÐ¸Ð°Ð»ÑÐ½ÑÐµ ÑÐµÐ°ÐºÑÐ¸Ð¸ ÑÐ¸ÐºÐ»Ð° ÑÐ»Ð¾Ð½Ð³Ð°ÑÐ¸Ð¸ ÑÑÐ°Ð½ÑÐ»ÑÑÐ¸Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: The effect of the antibiotic thermorubin on the elemental steps of the prokaryotic protein synthesis elongation cycle
Creators: name: ÐÑÐ°ÑÐµÐ², ÐÐ»ÐµÐºÑÐ°Ð½Ð´Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: ÑÐµÑÐ¼Ð¾ÑÑÐ±Ð¸Ð½
Ð±Ð¸Ð¾ÑÐ¸Ð½ÑÐµÐ· Ð±ÐµÐ»ÐºÐ°
ÑÐ¸Ð±Ð¾ÑÐ¾Ð¼Ð½ÑÐµ Ð°Ð½ÑÐ¸Ð±Ð¸Ð¾ÑÐ¸ÐºÐ¸
ÑÐ¸ÐºÐ» ÑÐ»Ð¾Ð½Ð³Ð°ÑÐ¸Ð¸
Ð¿ÑÐµÐ´ÑÑÐ°ÑÐ¸Ð¾Ð½Ð°ÑÐ½Ð°Ñ ÐºÐ¸Ð½ÐµÑÐ¸ÐºÐ°
Ð¿ÐµÐ¿ÑÐ¸Ð´Ð½Ð°Ñ ÑÐ²ÑÐ·Ñ
thermorubin
protein biosynthesis
ribosomal antibiotics
elongation cycle
rapid kinetics
peptide bond

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð° Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð°Ð½ÑÐ¸Ð±Ð¸Ð¾ÑÐ¸ÐºÐ¾Ð¼ ÑÐµÑÐ¼Ð¾ÑÑÐ±Ð¸Ð½Ð¾Ð¼ ÑÐ»Ð¾Ð½Ð³Ð°ÑÐ¸Ð¸ ÑÑÐ°Ð½ÑÐ»ÑÑÐ¸Ð¸. ÐÐµÑÐ¾Ð´Ð¾Ð¼ Ð¾ÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¿Ð¾ÑÐ¾ÐºÐ° Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ðµ ÑÐºÐ¾ÑÐ¾ÑÑÐ¸ Ð°ÐºÐºÐ¾Ð¼Ð¾Ð´Ð°ÑÐ¸Ð¸ Ð°Ð¼Ð¸Ð½Ð¾Ð°ÑÐ¸Ð»-ÑÐ ÐÐ Ð¿ÑÐ¸ ÑÐ²ÑÐ·ÑÐ²Ð°Ð½Ð¸Ð¸ Ð² Ð ÑÐ°Ð¹Ñ ÑÐ¸Ð±Ð¾ÑÐ¾Ð¼Ñ. ÐÐ¾ÐºÐ°Ð·Ð°Ð½Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð°Ð½ÑÐ¸Ð±Ð¸Ð¾ÑÐ¸ÐºÐ° Ð½Ð° ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð¸ ÐºÐ¸Ð½ÐµÑÐ¸ÐºÑ Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ñ Ð´Ð¸Ð¿ÐµÐ¿ÑÐ¸Ð´Ð°. ÐÐ¾Ð¼Ð¸Ð¼Ð¾ ÑÑÐ¾Ð³Ð¾, Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ñ ÑÐ°Ð·Ð»Ð¸ÑÐ¸Ñ Ð² Ð´ÐµÐ¹ÑÑÐ²Ð¸Ð¸ ÑÐµÑÐ¼Ð¾ÑÑÐ±Ð¸Ð½Ð° Ð½Ð° ÐºÐ¸Ð½ÐµÑÐ¸ÐºÑ ÑÐ²ÑÐ·ÑÐ²Ð°Ð½Ð¸Ñ ÑÐ°Ð·Ð½ÑÑ Ð°Ð¼Ð¸Ð½Ð¾Ð°ÑÐ¸Ð»-ÑÐ ÐÐ, Ð¾ÑÐ»Ð¸ÑÐ°ÑÑÐ¸ÑÑÑ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÑÑ Ð¸ ÑÑÐµÐ¿ÐµÐ½ÑÑ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾ÑÑÐ¸ Ð°Ð½ÑÐ¸ÐºÐ¾Ð´Ð¾Ð½Ð¾Ð²Ð¾Ð³Ð¾ Ð´Ð¾Ð¼ÐµÐ½Ð°. ÐÑÐ½Ð¾Ð²Ð½ÑÐµ Ð¼ÐµÑÐ¾Ð´Ñ, Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð½ÑÐµ Ð² Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ: in vitro ÑÐµÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ñ Ð±ÐµÐ»Ð¾ÐºÑÐ¸Ð½ÑÐµÐ·Ð¸ÑÑÑÑÐµÐ¹ ÑÐ¸ÑÑÐµÐ¼Ñ E. coli, Ð¸Ð·Ð¼ÐµÑÐµÐ½Ð¸Ðµ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÐ¾Ð² Ð¿ÑÐµÐ´ÑÑÐ°ÑÐ¸Ð¾Ð½Ð°ÑÐ½Ð¾Ð¹ ÐºÐ¸Ð½ÐµÑÐ¸ÐºÐ¸ ÑÐµÐ°ÐºÑÐ¸Ð¹ Ð¼ÐµÑÐ¾Ð´Ð°Ð¼Ð¸ Ð¾ÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¸ Ð¿Ð¾Ð³Ð°ÑÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¿Ð¾ÑÐ¾ÐºÐ°, Ð²ÑÑÐ¾ÐºÐ¾ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð°Ñ Ð¶Ð¸Ð´ÐºÐ¾ÑÑÐ½Ð°Ñ ÑÑÐ¾Ð¼Ð°ÑÐ¾Ð³ÑÐ°ÑÐ¸Ñ Ð² Ð¾Ð±ÑÐ°ÑÐµÐ½Ð½ÑÑ ÑÐ°Ð·Ð°Ñ, ÑÐ°Ð´Ð¸Ð¾Ð»ÑÐ¼Ð¸Ð½ÐµÑÑÐµÐ½ÑÐ½ÑÐ¹ ÑÐ¿ÐµÐºÑÑÐ°Ð»ÑÐ½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð·.
description: The present work is devoted to the study of the inhibition mechanism of translation elongation by antibiotic thermorubin. Decreasing effect on the rate of aminoacyl-tRNA accommodation to the A site was determined by the stopped flow method. The influence of the antibiotic on the efficiency and kinetics of dipeptide formation was shown. Furthermore, the differences in thermorubin action on the binding of aminoacyl-tRNAs with different sequences and degree of modification were determined. The main methods used in this work are in vitro reconstruction of the E. coli protein synthesis system, measurement of rapid kinetics parameters by the stopped or quenched flow methods, high-performance liquid chromatography in reversed phases, radioluminescent spectral analysis.