title: Ð¡Ð¾ÑÑÐ¾ÑÐ½Ð¸Ðµ ÐÐÐ ÐºÐ»ÐµÑÐºÐ¸-Ñ Ð¾Ð·ÑÐ¸Ð½Ð° Ð²Ð¾ Ð²ÑÐµÐ¼Ñ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸ Ð³Ð¸Ð³Ð°Ð½ÑÑÐºÐ¸Ð¼ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð¾Ð¼ ÏKZ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: State of host DNA during the infection of giant bacteriophage ÏKZ
Creators: name: ÐÐµÐ»Ð¾ÑÑÐ¾Ð²Ð°, ÐÐ¸ÐºÑÐ¾ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³
Ð¸Ð½ÑÐµÐºÑÐ¸Ñ
Ð´ÐµÐ³ÑÐ°Ð´Ð°ÑÐ¸Ñ ÐÐÐ
phiKZ
Pseudomonas aeruginosa
Ð¿ÑÐµÐ²Ð´Ð¾-ÑÐ´ÑÐ¾
ÐÐ¦Ð Ð² ÑÐµÐ°Ð»ÑÐ½Ð¾Ð¼ Ð²ÑÐµÐ¼ÐµÐ½Ð¸
bacteriophage
infection
DNA degradation
phage nucleus
real-time PCR

description: ÐÐ¸Ð³Ð°Ð½ÑÑÐºÐ¸Ð¹ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³ phiKZ Ð²Ð¾ Ð²ÑÐµÐ¼Ñ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸ Ð¾Ð±ÑÐ°Ð·ÑÐµÑ Ð²Ð½ÑÑÑÐ¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð¸-ÑÐ¾Ð·ÑÐ¸Ð½Ð° ÑÑÐµÑÐ¸ÑÐµÑÐºÑÑ ÑÑÑÑÐºÑÑÑÑ, ÑÐ¾ÑÑÐ¾ÑÑÑÑ Ð¸Ð· Ð±ÐµÐ»ÐºÐ¾Ð²Ð¾Ð¹ Ð¾Ð±Ð¾Ð»Ð¾ÑÐºÐ¸ Ð¸ ÐÐÐ. Ð­ÑÐ° ÑÑÑÑÐºÑÑÑÐ° ÑÐ´ÐµÑÐ¶Ð¸Ð²Ð°ÐµÑÑÑ Ð² ÑÐµÐ½ÑÑÐµ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð¹ ÐºÐ»ÐµÑÐºÐ¸ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÑÑÐ±ÑÐ»Ð¸Ð½Ð¾-Ð¿Ð¾Ð´Ð¾Ð±Ð½ÑÑ ÑÐ°Ð³Ð¾Ð²ÑÑ Ð±ÐµÐ»ÐºÐ¾Ð². ÐÐ½ÑÑÑÐ¸ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÑÑÑÐºÑÑÑÑ Ð±ÑÐ»Ð¸ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ñ Ð±ÐµÐ»ÐºÐ¸, ÑÑÐ°ÑÑÐ²ÑÑÑÐ¸Ðµ Ð² ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð¸ Ð¸ ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸Ð¸ ÐÐÐ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð°. Ð¢Ð°ÐºÐ¸Ð¼ Ð¾Ð±ÑÐ°Ð·Ð¾Ð¼, Ð´Ð°Ð½Ð½Ð°Ñ ÑÑÑÑÐºÑÑÑÐ° Ð¿Ð¾ ÑÐ²Ð¾ÐµÐ¹ ÑÐ¾ÑÐ¼Ðµ Ð¸ ÑÑÐ½ÐºÑÐ¸ÑÐ¼ Ð¿Ð¾ÑÐ¾Ð¶Ð° Ð½Ð° ÑÐ´ÑÐ¾ ÑÑÐºÐ°ÑÐ¸Ð¾ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ ÐºÐ»ÐµÑÐºÐ¸ Ð¸ Ð±ÑÐ»Ð° Ð½Ð°Ð·Ð²Ð°Ð½Ð° Â«Ð¿ÑÐµÐ²Ð´Ð¾-ÑÐ´ÑÐ¾Â». Ð¡ÑÑÐµÑÑÐ²ÑÐµÑ Ð³Ð¸Ð¿Ð¾ÑÐµÐ·Ð° Ð¾ ÑÐ¾Ð¼, ÑÑÐ¾ Ð²Ð¸ÑÑÑÑ, Ð¾Ð±ÑÐ°Ð·ÑÑÑÐ¸Ðµ Ð¿Ð¾Ð´Ð¾Ð±Ð½ÑÐµ ÑÑÑÑÐºÑÑÑÑ, Ð¼Ð¾Ð³Ð»Ð¸ ÑÑÐ³ÑÐ°ÑÑ Ð²Ð°Ð¶Ð½ÑÑ ÑÐ¾Ð»Ñ Ð² ÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¸Ð¸ ÑÑÐºÐ°ÑÐ¸Ð¾Ñ. ÐÐ·ÑÑÐµÐ½Ð¸Ðµ Ð°ÑÐ¿ÐµÐºÑÐ¾Ð² ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¿ÑÐµÐ²Ð´Ð¾-ÑÐ´ÑÐ° ÑÐ²Ð»ÑÐµÑÑÑ Ð°ÐºÑÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð·Ð°Ð´Ð°ÑÐµÐ¹ Ñ ÑÐ¾ÑÐºÐ¸ Ð·ÑÐµÐ½Ð¸Ñ ÑÐ²Ð¾Ð»ÑÑÐ¸Ð¸. ÐÑÑÐ°ÑÑ Ð²Ð¾Ð¿ÑÐ¾Ñ Ð¾ ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐ½Ð¾ÑÑÐ¸ ÑÐ¿Ð°ÐºÐ¾Ð²ÐºÐ¸ ÐÐÐ Ð²Ð½ÑÑÑÑ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÑÑÑÐºÑÑÑÑ. ÐÐ»Ñ Ð¾ÑÐ²ÐµÑÐ° Ð½Ð° ÑÑÐ¾Ñ Ð²Ð¾Ð¿ÑÐ¾Ñ Ð² Ð¿ÐµÑÐ²ÑÑ Ð¾ÑÐµÑÐµÐ´Ñ Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼Ð¾ Ð¾Ð¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ, Ð¿ÑÐ¾Ð¸ÑÑÐ¾Ð´Ð¸Ñ Ð»Ð¸ Ð´ÐµÐ³ÑÐ°Ð´Ð°ÑÐ¸Ñ ÐÐÐ Ð±Ð°ÐºÑÐµÑÐ¸Ð¸ Ð²Ð¾ Ð²ÑÐµÐ¼Ñ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸. Ð Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐ°Ð·Ð´ÐµÐ»ÐµÐ½Ð¸Ñ ÐÐÐ Ð¸ ÐÐ¦Ð  Ð² ÑÐµÐ°Ð»ÑÐ½Ð¾Ð¼ Ð²ÑÐµÐ¼ÐµÐ½Ð¸ Ð±ÑÐ»Ð¾ ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¾, ÑÑÐ¾ ÐÐÐ Ð±Ð°ÐºÑÐµÑÐ¸Ð¸ Ð½Ðµ Ð¿Ð¾Ð´Ð²ÐµÑÐ³Ð°ÐµÑÑÑ Ð´ÐµÐ³ÑÐ°Ð´Ð°ÑÐ¸Ð¸ Ð¿ÑÐ¸ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð¾Ð¼ phiKZ.
description: Giant bacteriophage phiKZ forms a spherical structure, containing a protein shell
and DNA, in its host bacteria cell during infection. This structure is positioned in the
center of the cell by tubulin-like phage proteins. Some protein participating in
replication and transcription of phage DNA were found inside this structure. Therefore,
this structure by its organization and function resembles a nucleus of a eukaryotic cell
and was called âpseudo-nucleusâ. A hypothesis was proposed that viruses that create
similar structures in ancient cells may have played an important role in evolution of
eukaryotes. Studying the functioning of pseudo-nucleus is an important problem for an
evolutionary point of view. First arises a question about specificity of DNA packing
into the pseudo-nucleus. To answer that question, it needs to be determined whether
bacterial DNA undergoes degradation in an infected cell. In this work DNA gel-electrophoresis and real-time PCR were used to establish that bacterial DNA does not
undergo degradation during the infection of phiKZ bacteriophage.