Plasma responses in human subjects after ingestions of multiple doses of natural α-cryptoxanthin: A pilot study
Creators: name: Schlatterer, Jörg
name: Breithaupt, Dietmar E.
name: Wolters, Maike
name: Hahn, Andreas
Publisher: name: Cambridge : Cambridge University Press
Year: 2006
Subjects: subject: α-Cryptoxanthin
subject: Liquid chromatography-mass spectroscopy in the single ion monitoring mode
subject: Pilot studies
subject: Plasma xanthophyll response
subject: alpha cryptoxanthin
subject: carotenoid
subject: cellulose
subject: cholesterol
subject: cryptoxanthin
subject: gelatin
subject: triacylglycerol
subject: unclassified drug
subject: xanthophyll
subject: beta carotene
subject: drug derivative
subject: adult
subject: article
subject: blood sampling
subject: cholesterol blood level
subject: drug absorption
subject: drug blood level
subject: drug capsule
subject: drug formulation
subject: drug isolation
subject: high performance liquid chromatography
subject: human
subject: intestine absorption
subject: liquid chromatography
subject: male
subject: mass spectrometry
subject: multiple drug dose
subject: pilot study
subject: anthropometry
subject: blood
subject: drug administration
subject: isolation and purification
subject: methodology
subject: microcapsule
subject: oral drug administration
subject: Administration, Oral
subject: Adult
subject: Anthropometry
subject: beta Carotene
subject: Capsules
subject: Chromatography, High Pressure Liquid
subject: Chromatography, Liquid
subject: Drug Administration Schedule
subject: Humans
subject: Intestinal Absorption
subject: Male
subject: Mass Spectrometry
subject: Pilot Projects
subject: Xanthophylls
subject: Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit

description: Xanthophylls have attracted a lot of interest since their health benefits were documented. Unfortunately, studying their intestinal absorption is often affected by high baseline levels present in the fasting plasma. As a-cryptoxanthin is rarely found in the traditional European diet, its concentration in human plasma is extremely low. A pilot human intervention study was designed using a-cryptoxanthin for the first time as a marker xanthophyll in a minimally formulated cellulose-based supplement. α-Cryptoxanthin was administered in gelatin soft-gel capsules in multiple doses of 156 μg/d to three male volunteers (age 27.3 (SD 4.7) years; BMI 21.6 (SD 0.3) kg/m2) for 16 d after a 2-week carotenoid depletion period. Fasting blood samples were taken before the intervention and after 3, 6, 9, 13 and 16 d. Plasma HPLC analyses allowed for determination of the concentration; liquid chromatography-MS in the single ion monitoring mode was used to confirm peak assignment. The concentrations of α-cryptoxanthin increased significantly after only 3 d of supplementation. The concentration-time plots showed a characteristic shape with a first maximum after day 6, a decline until day 9 and a gradual second rise until the end of the study. standardisation of plasma α-cryptoxanthin concentrations to triacylglycerol or total cholesterol did not influence the characteristics. The maximum concentrations reached at the end of the intervention period ranged from 0.077 to 0.160 μmol/l. These results suggest a high intestinal absorption and an enrichment of α-cryptoxanthin in the plasma even from a minimally formulated cellulose-based supplement. © The Authors 2006.