title: ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð±Ð¸Ð¾ÑÐ¾Ð²Ð¼ÐµÑÑÐ¸Ð¼Ð¾ÑÑÐ¸ 3D ÑÐºÐ°ÑÑÐ¾Ð»Ð´Ð¾Ð² Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ Ð¿ÑÐ¸ÑÐ¾Ð´Ð½ÑÑ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ¾Ð² Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÑÐµÐ¼ÑÐ¼Ð¸ ÐºÐ»ÐµÑÐºÐ°Ð¼Ð¸ Ð² ÑÐµÐ»ÑÑ ÑÐ¾Ð·Ð´Ð°Ð½Ð¸Ñ Ð±Ð¸Ð¾Ð¼ÐµÐ´Ð¸ÑÐ¸Ð½ÑÐºÐ¸Ñ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð¿ÑÐ¾Ð´ÑÐºÑÐ¾Ð² Ð´Ð»Ñ ÑÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸Ð²Ð½Ð¾Ð¹ Ð¼ÐµÐ´Ð¸ÑÐ¸Ð½Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Research of the biocompatibility of 3D scaffolds based on natural polymers with cultured cells in order to create biomedical cell products for regenerative medicine
Creators: name: ÐÑÐµÑÐ°Ð½Ð°Ñ, Ð®Ð»Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: Ð±Ð¸Ð¾Ð¼ÐµÐ´Ð¸ÑÐ¸Ð½ÑÐºÐ¸Ð¹ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐ¹ Ð¿ÑÐ¾Ð´ÑÐºÑ
ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ð¾Ð²ÑÐ¹ Ð³Ð¸Ð´ÑÐ¾Ð³ÐµÐ»Ñ
Ð±Ð¸Ð¾ÑÐ¾Ð²Ð¼ÐµÑÑÐ¸Ð¼Ð¾ÑÑÑ
ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ ÐºÑÐ»ÑÑÑÑ
ÑÐµÑÐ¿Ð¸ÑÐµÐ»Ð¸Ð·Ð°ÑÐ¸Ñ
ÑÐºÐ°Ð½ÐµÐ²Ð°Ñ Ð¸Ð½Ð¶ÐµÐ½ÐµÑÐ¸Ñ
ÑÐ¸Ð½Ð´ÑÐ¾Ð¼ Ð»Ð¸Ð¼Ð±Ð°Ð»ÑÐ½Ð¾Ð¹ Ð½ÐµÐ´Ð¾ÑÑÐ°ÑÐ¾ÑÐ½Ð¾ÑÑÐ¸
biomedical cell product
collagen hydrogel
biocompatibility
cell culturing
reepithelization
tissue engineering
limbal stem deficiency

description: Ð¦ÐµÐ»ÑÑ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÐ»Ð°ÑÑ Ð¾ÑÐµÐ½ÐºÐ° Ð±Ð¸Ð¾ÑÐ¾Ð²Ð¼ÐµÑÑÐ¸Ð¼Ð¾ÑÑÐ¸ ÑÐºÐ°ÑÑÐ¾Ð»Ð´Ð¾Ð² Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ð¾Ð²Ð¾Ð³Ð¾ Ð³Ð¸Ð´ÑÐ¾Ð³ÐµÐ»Ñ Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÑÐµÐ¼ÑÐ¼Ð¸ Ð²Ð½ÑÑÑÐ¸ Ð½ÐµÐ³Ð¾ ÐºÐ»ÐµÑÐºÐ°Ð¼Ð¸. Ð ÑÐ°Ð¼ÐºÐ°Ñ Ð¿ÑÐ¾Ð²ÐµÐ´ÑÐ½Ð½Ð¾Ð³Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¸ Ð¿ÑÐ¸Ð³Ð¾ÑÐ¾Ð²Ð»ÐµÐ½Ñ ÑÑÐ¸ Ð²Ð°ÑÐ¸Ð°Ð½ÑÐ° ÑÐºÐ°Ð½ÐµÐ¸Ð½Ð¶ÐµÐ½ÐµÑÐ½ÑÑ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ð¹ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ð¾Ð²Ð¾Ð³Ð¾ Ð³Ð¸Ð´ÑÐ¾Ð³ÐµÐ»Ñ, Ð»Ð¸Ð¼Ð±Ð°Ð»ÑÐ½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº, ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐ»Ð¸Ð·Ð¸ÑÑÐ¾Ð¹ Ð¿Ð¾Ð»Ð¾ÑÑÐ¸ ÑÑÐ° Ð¸ Ð´ÐµÑÐ¼Ð°Ð»ÑÐ½ÑÑ ÑÐ¸Ð±ÑÐ¾Ð±Ð»Ð°ÑÑÐ¾Ð² ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°. Ð¡ÑÐ°Ð½Ð´Ð°ÑÑÐ½ÑÐµ ÐºÐ¾Ð»Ð¾ÑÐ¸Ð¼ÐµÑÑÐ¸ÑÐµÑÐºÐ¸Ðµ Ð¼ÐµÑÐ¾Ð´Ñ (ÐÐ¢Ð¢- Ð¸ ÑÐµÐ·Ð°Ð·ÑÑÐ¸Ð½-ÑÐµÑÑÑ) Ð¾ÑÐµÐ½ÐºÐ¸ Ð¶Ð¸Ð·Ð½ÐµÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÐ¸ ÐºÐ»ÐµÑÐ¾Ðº Ð±ÑÐ»Ð¸ Ð°Ð´Ð°Ð¿ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ñ Ð¿Ð¾Ð´ 3D ÑÑÐ»Ð¾Ð²Ð¸Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÐºÐ»ÐµÑÐ¾Ðº. ÐÑÐ»Ð° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð¾ÑÐµÐ½ÐºÐ° Ð¿ÑÐ¾Ð»Ð¸ÑÐµÑÐ°ÑÐ¸Ð²Ð½ÑÑ ÑÐ²Ð¾Ð¹ÑÑÐ² ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÑÐµÐ¼ÑÑ Ð²Ð½ÑÑÑÐ¸ ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ð¾Ð²Ð¾Ð³Ð¾ Ð³Ð¸Ð´ÑÐ¾Ð³ÐµÐ»Ñ ÐºÐ»ÐµÑÐ¾Ðº Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð°Ð´Ð°Ð¿ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ ÐÐ¢Ð¢- Ð¸ ÑÐµÐ·Ð°Ð·ÑÑÐ¸Ð½-ÑÐµÑÑÐ¾Ð². Ð¢Ð°ÐºÐ¶Ðµ Ð±ÑÐ»Ð° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð¾ÑÐµÐ½ÐºÐ° Ð¶Ð¸Ð·Ð½ÐµÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÐ¸ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ ÑÐ¸Ð¿Ð¾Ð² ÐºÐ»ÐµÑÐ¾Ðº Ð²Ð½ÑÑÑÐ¸ ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ð¾Ð²Ð¾Ð³Ð¾ Ð³Ð¸Ð´ÑÐ¾Ð³ÐµÐ»Ñ Ð¿Ð¾ Ð¸Ñ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð¼Ñ ÑÐ¾ÑÑÐ¾ÑÐ½Ð¸Ñ Ð¸ Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð°ÐºÑÐ¸Ð½Ð¾Ð²Ð¾Ð³Ð¾ ÑÐ¸ÑÐ¾ÑÐºÐµÐ»ÐµÑÐ°, Ð¾ÑÐµÐ½ÐµÐ½Ð° ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ðº Ð¸Ð½Ð´ÑÐºÑÐ¸Ð¸ ÐºÐ¾Ð½ÑÑÐ°ÐºÑÐ¸Ð¸ ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ð¾Ð²Ð¾Ð³Ð¾ Ð³Ð¸Ð´ÑÐ¾Ð³ÐµÐ»Ñ Ð¸ Ð¼Ð¸Ð³ÑÐ°ÑÐ¸Ð¸ Ð¸Ð· Ð½ÐµÐ³Ð¾. ÐÑÐ»Ð¾ Ð²ÑÑÐ²Ð»ÐµÐ½Ð¾, ÑÑÐ¾ ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ð¾Ð²ÑÐ¹ Ð³Ð¸Ð´ÑÐ¾Ð³ÐµÐ»Ñ ÑÐ²Ð»ÑÐµÑÑÑ Ð±Ð¸Ð¾ÑÐ¾Ð²Ð¼ÐµÑÑÐ¸Ð¼ÑÐ¼ ÑÐºÐ°ÑÑÐ¾Ð»Ð´Ð¾Ð¼ ÐºÐ°Ðº Ð´Ð»Ñ Ð´ÐµÑÐ¼Ð°Ð»ÑÐ½ÑÑ ÑÐ¸Ð±ÑÐ¾Ð±Ð»Ð°ÑÑÐ¾Ð², ÑÐ°Ðº Ð¸ Ð´Ð»Ñ Ð»Ð¸Ð¼Ð±Ð°Ð»ÑÐ½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð¸ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐ»Ð¸Ð·Ð¸ÑÑÐ¾Ð¹ Ð¿Ð¾Ð»Ð¾ÑÑÐ¸ ÑÑÐ° ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°, ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÑÐµÐ¼ÑÑ Ð²Ð½ÑÑÑÐ¸ Ð½ÐµÐ³Ð¾, Ð¸ Ð¼Ð¾Ð¶ÐµÑ Ð±ÑÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½ Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ ÑÐºÐ°ÑÑÐ¾Ð»Ð´Ð° Ð² ÑÐºÐ°Ð½ÐµÐ¸Ð½Ð¶ÐµÐ½ÐµÑÐ½ÑÑ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸ÑÑ Ð¿ÑÐ¸ ÑÐ¾Ð·Ð´Ð°Ð½Ð¸Ð¸ Ð±Ð¸Ð¾Ð¼ÐµÐ´Ð¸ÑÐ¸Ð½ÑÐºÐ¸Ñ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð¿ÑÐ¾Ð´ÑÐºÑÐ¾Ð² Ð½Ðµ ÑÐ¾Ð»ÑÐºÐ¾ Ð´Ð»Ñ ÑÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸Ð¸ ÐºÐ¾Ð¶Ð¸, Ð½Ð¾ Ð¸ Ð² Ð¾ÑÑÐ°Ð»ÑÐ¼Ð¾Ð»Ð¾Ð³Ð¸Ð¸ Ð´Ð»Ñ ÑÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸Ð¸ ÑÐ¾Ð³Ð¾Ð²Ð¸ÑÑ.
description: The given work is devoted to evaluating the biocompatibility of collagen-based scaffolds with cells cultured inside them. As part of the study, three variants of tissue-engineered structures based on collagen hydrogel, limbal stem cells, oral mucosa stem cells, and human dermal fibroblasts were prepared. Standard colorimetric methods (MTT and resazurin tests) for assessing cell viability were adapted to the 3D conditions of cell culture. The proliferative properties of the cells cultured inside the collagen hydrogel were also evaluated using adapted MTT and resazurin tests. The viability of various cell types inside the collagen hydrogel was also evaluated based on their morphological state and the organization of the actin cytoskeleton, and the ability of cells to induce collagen hydrogel contraction and migration from it was evaluated. It was found that the collagen hydrogel is a biocompatible scaffold for both dermal fibroblasts and limbal stem cells and stem cells of the human oral mucosa cultured inside it and can be used as a scaffold in tissue engineering structures when creating biomedical cell products not only for skin regeneration, but also in ophthalmology for corneal regeneration.