cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
(A)dS Holography with a Cut-off
Creators: name: Sarkar, Debajyoti
Publisher: name: arXiv
Year: 2014
Subjects: subject: High Energy Physics - Theory (hep-th)
subject: FOS: Physical sciences

description: We find out the smearing/ transfer functions that relate a local bulk operator with its boundary values at a cut-off surface located at $z=z_0$ of the AdS Poincaré patch. We compare these results with de Sitter counterparts and comment on their connections with corresponding construction for dS/ CFT. As the boundary values can help define the required field theory at $z=z_0$ and encode bulk locality in terms of it, our work can provide key information about holographic RG in the context of AdS/ CFT.
description: v.2 Minor modifications over v.1. Journal version. 32 pages, 3 figures
Independent deletions of a pathogen-resistance gene in Brassica and Arabidopsis
Creators: name: Grant, M. R.
name: de Torres Zabala, M.
name: Dangl, J. L.
name: Lydiate, D. J.
name: Sharpe, A. G.
name: McDowell, J. M.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1998

description: Plant disease resistance (R) genes confer race-specific resistance to pathogens and are genetically defined on the basis of intra-specific functional polymorphism. Little is known about the evolutionary mechanisms that generate this polymorphism. Most R loci examined to date contain alternate alleles and/or linked homologs even in disease-susceptible plant genotypes. In contrast, the resistance to Pseudomonas syringae pathovar maculicola (RPM1) bacterial resistance gene is completely absent (rpm1-null) in 5/5 Arabidopsis thaliana accessions that lack RPM1 function. The rpm1-null locus contains a 98-bp segment of unknown origin in place of the RPM1 gene. We undertook comparative mapping of RPM1 and flanking genes in Brassica napus to determine the ancestral state of the RPM1 locus. We cloned two B. napus RPM1 homologs encoding hypothetical proteins with ≈81% amino acid identity to Arabidopsis RPM1. Collinearity of genes flanking RPM1 is conserved between B. napus and Arabidopsis. Surprisingly, we found four additional B. napus loci in which the flanking marker synteny is maintained but RPM1 is absent. These B. napus rpm1-null loci have no detectable nucleotide similarity to the Arabidopsis rpm1-null allele. We conclude that RPM1 evolved before the divergence of the Brassicaceae and has been deleted independently in the Brassica and Arabidopsis lineages. These results suggest that functional polymorphism at R gene loci can arise from gene deletions.
Mice with type 2 and 3 Gaucher disease point mutations generated by a single insertion mutagenesis procedure (SIMP)
Creators: name: Grinberg, A.
name: Suzuki, K.
name: Doring, T.
name: Sandhoff, K.
name: Proia, R. L.
name: Hoffmann, A.
name: Westphal, H.
name: Liu, Y.
name: Reed, J. D.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1998

description: Gaucher disease is caused by mutations in the gene encoding the lysosomal enzyme glucocerebrosidase (GC). Three clinical types of Gaucher disease have been defined according to the presence (type 2 and 3) or absence (type 1) of central nervous system disease and severity of clinical manifestations. The clinical course of the disease correlates with the mutation carried by the GC gene. To produce mice with point mutations that correspond to the clinical types of Gaucher disease, we have devised a highly efficient one-step mutagenesis method—the single insertion mutagenesis procedure (SIMP)—to introduce human disease mutations into the mouse GC gene. By using SIMP, mice were generated carrying either the very severe RecNciI mutation that can cause type 2 disease or the less severe L444P mutation associated with type 3 disease. Mice homozygous for the RecNciI mutation had little GC enzyme activity and accumulated glucosylceramide in brain and liver. In contrast, the mice homozygous for the L444P mutation had higher levels of GC activity and no detectable accumulation of glucosylceramide in brain and liver. Surprisingly, both point mutation mice died within 48 hr of birth, apparently of a compromised epidermal permeability barrier caused by defective glucosylceramide metabolism in the epidermis.
On the Claims of Jordan Peterson's Complexity Management Theory
Creators: name: Barros, Michael
Publisher: name: figshare
Year: 2023
Subjects: Other psychology not elsewhere classified

description: Dr. Jordan B. Peterson‟s ideas have been digested by millions of people around the world. He‟s been described by the New York Times as “the most influential public intellectual in the Western world right now.” (Brooks, 2018) He covers a lot of ground, from Disney movies to Nietzsche to Biblical narratives, but connecting all of these is a golden thread: Complexity Management Theory. Complexity Management Theory is the name given to a neuropsychological framework for describing human perception and meaning in an overly complex world. While the name hasn‟t stuck, the model itself has been refined and rearticulated several times since then. If it‟s accurate, such a comprehensive framework has undeniable utility, if it‟s inaccurate, then the most influential public intellectual in the Western world has based a great deal of his lectures on a false premise. This essay summarizes and critiques Complexity Management Theory as it stands today, contextualizing and critiquing its central tenets as they relate to their respective fields. The results of this inquiry indicate that Complexity Management Theory is both well- substantiated and useful, but it requires further elaboration on its views of perception.
Carbon-rich dust past the asymptotic giant branch: aliphatics, aromatics, and fullerenes in the Magellanic Clouds
Creators: name: Sloan, G. C.
name: Lagadec, E.
name: Zijlstra, A. A.
name: Kraemer, K. E.
name: Weis, A. P.
name: Matsuura, M.
name: Volk, K.
name: Peeters, E.
name: Duley, W. W.
name: Cami, J.
name: Bernard-Salas, J.
name: Kemper, F.
name: Sahai, R.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Solar and Stellar Astrophysics (astro-ph.SR)
subject: FOS: Physical sciences

description: Infrared spectra of carbon-rich objects which have evolved off the asymptotic giant branch reveal a range of dust properties, including fullerenes, polycyclic aromatic hydrocarbons (PAHs), aliphatic hydrocarbons, and several unidentified features, including the 21 um emission feature. To test for the presence of fullerenes, we used the position and width of the feature at 18.7-18.9 um and examined other features at 17.4 and 6-9 um. This method adds three new fullerene sources to the known sample, but it also calls into question three previous identifications. We confirm that the strong 11 um features seen in some sources arise primarily from SiC, which may exist as a coating around carbonaceous cores and result from photo-processing. Spectra showing the 21 um feature usually show the newly defined Class D PAH profile at 7-9 um. These spectra exhibit unusual PAH profiles at 11-14 um, with weak contributions at 12.7 um, which we define as Class D1, or show features shifted to ~11.4, 12.4, and 13.2 um, which we define as Class D2. Alkyne hydrocarbons match the 15.8 um feature associated with 21 um emission. Sources showing fullerene emission but no PAHs have blue colors in the optical, suggesting a clear line of sight to the central source. Spectra with 21 um features and Class D2 PAH emission also show photometric evidence for a relatively clear line of sight to the central source. The multiple associations of the 21 um feature to aliphatic hydrocarbons suggest that the carrier is related to this material in some way.
description: 30 pages, 30 figures, ApJ, in press
OpenFF Follow Up Workshop: "Interchange Showcase"
Creators: name: Thompson, Matt
Publisher: name: Zenodo
Year: 2022

description: The "Interchange Showcase" followup workshop presented by Matt Thompson of OpenFF. This session will cover the current state of Interchange development and future plans. The example code will explore the API points for creating, inspecting, modifying, combining, and exporting parameterized molecular systems using the new Interchange object, and show examples of how Interchange can be tied into larger workflows. The material from this workshop is available at https://github.com/openforcefield/interchange-workshop-2022/tree/2022.0.0 This video is also available on YouTube at https://youtu.be/YA3PpeFFcqk
description: This work was supported by NIH Grant NIH R01 GM132386.
Sunday league football and potential for social change
Creators: name: Tucker, Lee
Publisher: name: Leeds Beckett University
Year: 2022
Subjects: Uncategorized

description: This thesis examines the role and importance of football in the lives of players from a Sunday league football club representing a pub in the north-east of England. It considers the extent to which the club provides a context for social change and what influences this. The study employs a predominantly Bourdieusian analysis to consider how various forms of capital are accumulated and operationalised by different individuals within the club to gain status and influence. The thesis also evaluates the researcher’s role(s) within the club, particularly how leadership and social change can be understood as an interdependent dynamic. Drawing on a three-year ethnographic study, influenced by the researcher’s role as founder and manager of the club, a deep insider status gave access to all aspects of club life. A research diary was maintained, supported by eight semi-structured interviews with two or three participants in each interview, culminating in 21 people in total. Interviews were conducted to explore players’ and supporters’ views, provided first-hand accounts of which forms of capital are valued within the club and directly informed the analysis of how successful the club was in achieving social change. Further, narratives based on the researcher’s experiences, provide insight into embedded and embodied relationships with football. The thesis concludes that football continues to be a significant aspect of habitus for individuals and the community at the centre of this study. Football shapes and influences dispositions, values and lives. In a wider context, the thesis locates football as an opiate, distracting players and supporters from other, potentially counter-hegemonic, activity. Conceptually, bonding social capital is regarded as critical in the sense of belonging created within the club. Embodied cultural capital is critical in influencing behaviour, status and relationships within the club. The thesis introduces football capital as an emergent concept complementary to other forms of capital. Social change has occurred at the club, helped by adherence to a club Constitution and dialogue with influential members of the club. Data demonstrate that individuals with significant football capital who consciously adopt a sociology of leadership approach can be influential in instigating social change, including challenging dominant dispositions and learned values, with a resulting emphasis on inclusivity and equality and that can be adopted and embodied in a community based football club. It is concluded that the club here, understood as a cultural field, can be regarded as successful at resisting and challenging dominant hegemonies to an extent.
Molecular cloning and characterization of an invertebrate cellular retinoic acid binding protein
Creators: name: Muehleisen, D. P.
name: Bollenbacher, W. E.
name: Alexander, S. C.
name: Mansfield, S. G.
name: Cammer, S.
name: Tropsha, A.
name: Gray, R. S.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1998

description: We have cloned a cDNA and gene from the tobacco hornworm, Manduca sexta, which is related to the vertebrate cellular retinoic acid binding proteins (CRABPs). CRABPs are members of the superfamily of lipid binding proteins (LBPs) and are thought to mediate the effects of retinoic acid (RA) on morphogenesis, differentiation, and homeostasis. This discovery of a Manduca sexta CRABP (msCRABP) demonstrates the presence of a CRABP in invertebrates. Compared with bovine/murine CRABP I, the deduced amino acid sequence of msCRABP is 71% homologous overall and 88% homologous for the ligand binding pocket. The genomic organization of msCRABP is conserved with other CRABP family members and the larger LBP superfamily. Importantly, the promoter region contains a motif that resembles an RA response element characteristic of the promoter region of most CRABPs analyzed. Three-dimensional molecular modeling based on postulated structural homology with bovine/murine CRABP I shows msCRABP has a ligand binding pocket that can accommodate RA. The existence of an invertebrate CRABP has significant evolutionary implications, suggesting CRABPs appeared during the evolution of the LBP superfamily well before vertebrate/invertebrate divergence, instead of much later in evolution in selected vertebrates.
Natriuretic peptide receptor 1 expression influences blood pressures of mice in a dose-dependent manner
Creators: name: Oliver, P. M.
name: Goy, M. F.
name: Purdy, K. E.
name: Smithies, O.
name: Kim, R.
name: Maeda, N.
name: John, S. W. M.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1998

description: Activation of the natriuretic peptide system lowers blood pressure and causes the excretion of salt. Atrial natriuretic peptide and B-type natriuretic peptide are the humoral mediators of this effect; they act primarily by binding to membrane-bound natriuretic peptide receptor A (NPRA) and stimulating its intrinsic guanylate cyclase activity. To study whether genetically determined differences in NPRA expression affect blood pressure we have generated mice with one, two, three, or four copies of the gene encoding NPRA (Npr1 in the mouse). Atrial natriuretic peptide-dependent guanylate cyclase activity ranged progressively from approximately one-half normal in one-copy animals to twice normal in four-copy animals (P < 0.001). On different diets (0.05%, 2%, and 8% NaCl), the blood pressures of F1 male mice having only one copy of Npr1 averaged 9.1 mmHg (1 mmHg = 133 Pa) above those of wild-type two-copy males (P < 0.001), whereas males with three copies of the gene had blood pressures averaging 5.2 mmHg below normal (P < 0.01). The blood pressures of the one-copy F1 animals were significantly higher (by 6.2 mmHg; P < 0.01) on the high-salt than on the low-salt diet. The blood pressures of four-copy F3 males were significantly lower (by 7 mmHg; P < 0.05) on the high-salt than on the low-salt diet. These results demonstrate that below normal Npr1 expression leads to a salt-sensitive increase in blood pressure, whereas above normal Npr1 expression lowers blood pressures and protects against high dietary salt.
Transforming or reproducing an unequal economy? Solidarity andinequality in a community currency
Creators: name: Barinaga, Ester
Publisher: name: International Journal of Community Currency Research (IJCCR)
Year: 2019
Subjects: mutual credit currency
inequality
Ostrom
resource system vs. flow of resource units
provision / appropriation ratio
common-pool resource

description: Building on empirical material from 6 months ethnographically inspiredfieldwork in Málaga Común, a mutual credit community currency inSouthern Spain, the paper uses Ostrom’s (1991) theoretical frameworkon common-pool resources to look deeper into the provision andappropriation dynamics in the currency scheme. Particular attention isput into the sources of inequality in members’ provision andappropriation capacities. Findings suggest that, embedded as communitycurrencies are in the conventional economy, the sources of inequalityfrom the conventional economy are also brought into the communitycurrency. More particularly, private ownership and specialised complexskills lie behind members’ unequal capacity to earn community currencyin relation to their spending needs. The paper ends by outlining someelements that would need attention when designing the governanceinstitutions of community currency schemes that aim to overcome theinequality brought in by these currencies’ embeddedness in theconventional economy
Additional file 1 of Several implications for the pathogenesis and treatment of thrombosis in PNH patients according to multiomics analysis
Creators: name: Chen, Yingying
name: Meng, Xiaorui
name: Wang, Yinxing
name: Liu, Chunyan
name: Fu, Rong
Publisher: name: figshare
Year: 2024
Subjects: subject: Physical Sciences not elsewhere classified
subject: Molecular Biology
subject: Pharmacology
subject: Ecology
subject: FOS: Biological sciences
subject: Biological Sciences not elsewhere classified
subject: Science Policy
subject: Hematology
subject: Plant Biology

description: Additional file 1: Materials & Methods. Figure S1. A. Dot plot displaying the expression level of marker genes in 14 clusters. B. Volcano plot showing differentially expressed genes (DEGs) across platelets in the PT and P groups. A total of 268 DEGs were identified using the Wilcoxon rank sum test with an absolute log2-fold change (FC) ≥ 0.5 and a p value < 0.05, including 193 upregulated genes in the PT group and 75 upregulated genes in the P group. C. GO enrichment analysis of DEGs in platelets. The color of the bubble indicates the Bonferroni-adjusted p value of the GO term, and the size of the bubble signifies the GeneRatio associated with a term. D. Volcano plot showing differentially expressed proteins (DEPs) between the PT and P groups. A total of 131 DEPs were identified using a fold change > 1.5 and a Q value < 0.05, including 79 upregulated proteins in the PT group and 52 upregulated proteins in the P group. E. KEGG enrichment analysis of DEPs. The color of the bubble indicates the -log10 (p value) of the KEGG terms, and the size of the bubble signifies the GeneRatio associated with a term. Table S1. Clinical characteristics of 8 PNH patients. Table S2. Annotation information of mutant gene in PT group.
Electric Octupole Order in Bilayer Ruthenate Sr$_3$Ru$_2$O$_7$
Creators: name: Hitomi, Takanori
name: Yanase, Youichi
Publisher: name: arXiv
Year: 2014
Subjects: subject: Strongly Correlated Electrons (cond-mat.str-el)
subject: FOS: Physical sciences

description: Although the odd-parity multipole order barely occurs in crystals with high symmetry, it can be formed in locally noncentrosymmetric crystals. We illustrate the odd-parity electric octupole order generated in a bilayer structure. When the local electric quadrupole is alternatively stacked between layers, it is regarded as an electric octupole order from the viewpoint of symmetry. We show that the $p_y s_x + p_x s_y$ spin nematic order is induced by the spin-orbit coupling in the electric octupole state, and it results from the spontaneous inversion symmetry breaking leading to the $D_{2d}$ point group symmetry. We investigate the possible realization of the electric octupole order in the bilayer ruthenate Sr$_3$Ru$_2$O$_7$, assuming a local electric quadrupole arising from the Pomeranchuk instability and/or the orbital order. Effects of the lattice distortion and magnetic field are also clarified, and the nature of the "electronic nematic state" of Sr$_3$Ru$_2$O$_7$ is discussed. It is proposed that the asymmetric band structure is a signature of the electric octupole order in Sr$_3$Ru$_2$O$_7$. The odd-parity multipole order in other strongly correlated electron systems is discussed.
description: Final version for publication in J. Phys. Soc. Jpn
Andere Stimmen - Frauen in den Weltreligionen
Creators: name: Haker, Hille
name: Ross, Susan A.
name: Wacker, Marie-Theres
Publisher: name: Concilium
Year: 1970

description: Concilium, Bd. 42 Nr. 3 (2006): Concilium: Internationale Zeitschrift für Theologie, Band 42, Heft 3
Plasticity of the neoplastic phenotype in vivo is regulated by epigenetic factors
Creators: name: Wilson, J. W.
name: Smith, G. J.
name: McCullough, K. D.
name: Ricketts, S. L.
name: Grisham, J. W.
name: Coleman, W. B.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1998

description: Age of host and transplantation-site microenvironment influence the tumorigenic potential of neoplastically transformed liver epithelial cells. Tumorigenic BAG2-GN6TF rat liver epithelial cells consistently form tumors at ectopic sites, but differentially express tumorigenicity or hepatocytic differentiation in the liver depending on host age and route of cell transplantation into the liver. Direct inoculation into host livers concentrates tumor cells locally, resulting in undifferentiated tumors near the transplantation site in both young (3-month-old) and old (18-month-old) rats. Transplantation-site tumors regress within 1 month in the livers of young rats, but grow progressively in old rats. However, inoculation of cells into the spleen distributes transplanted cells individually throughout the liver, resulting in hepatocytic differentiation by tumor cells with concomitant suppression of their tumorigenicity in young rats. When transplanted into livers of old rats by splenic inoculation, or when young hepatic-transplant recipients are allowed to age, hepatocytic progeny of BAG2-GN6TF cells proliferate to form foci, suggesting that the liver microenvironment of old rats incompletely regulates the proliferation and differentiation of tumor cell-derived hepatocytes. Upon removal from the liver, BAG2-GN6TF-derived hepatocytes revert to an undifferentiated, aggressively tumorigenic phenotype. We posit that the spectrum between normal differentiation and malignant potential of these cells reflects the dynamic interaction of the specific transformation-related genotype of the cells and the characteristics of the tissue microenvironment at the transplantation site. Changes in the tissue milieu, such as those that accompany normal aging, may determine the ability of a genetically aberrant cell to produce a tumor.
File Fixity in the Cloud: Policy, Business, and Technical Considerations
Creators: name: Rimkus, Kyle
name: Genevieve, Schmitt
Publisher: name: Zenodo
Year: 2024

description: When shifting storage managed on-premises into the cloud, many digital preservation repository managers must reevaluate their approach to guaranteeing file integrity. Most cloud storage vendors offer guarantees of file durability that call into question the need for continuous fixity checking. At the same time, cloud vendors tend to charge fees for bringing computational resources to bear on individual files, turning continuous fixity checking into a potentially burdensome expense. With these technical and business considerations in mind, this paper explores one institution’s solution to verifying fixity in a repository of approximately seventeen million files after shifting on-site storage into the cloud. It evaluates broader considerations for the field of digital preservation when considering file integrity in the cloud and concludes with an explanation of a local policy for affordable ongoing fixity monitoring adapted to the affordances of a cloud-adapted architecture.
Learning Deep Feature Representations of 3D Point Cloud Data
Creators: name: Qiu, Shi
Publisher: name: The Australian National University
Year: 2023

description: As 3D data acquisition techniques develop rapidly, different types of 3D scanners, like LiDAR scanners and RGB-D cameras, are becoming popular in our daily life. More importantly, 3D scanners can capture data that enables intelligent machines to better see and recognize the world. As a fundamental 3D data representation, point clouds can be easily collected using 3D scanners, retaining abundant information for AI-driven applications such as autonomous driving, virtual/augmented reality, and robotics. Given the prominence of deep neural networks in current days, deep learning-based point cloud data understanding is playing an essential role in 3D computer vision research. In this thesis, we focus on learning deep feature representations of point clouds for 3D data processing and analysis. Basically, we start from investigating low-level vision problems of 3D point clouds, which helps to comprehend and deal with the inherent sparsity, irregularity and unorderedness of this 3D data type. On this front, we introduce a novel transformer-based model that fully utilizes the dependencies between scattered points for high-fidelity point cloud upsampling. Moreover, we deeply explore high-level vision problems of point cloud analysis, including the classification, segmentation and detection tasks. Specifically, we propose to (i) learn more geometric information for accurate point cloud classification by enriching geometric context in low-level 3D space and high-level feature spaces with an attention-based error-correcting feedback structure; (ii) exploit dense-resolution features for small-scale point cloud recognition using a novel local grouping method and a local feature error-minimizing module; (iii) augment local context for large-scale point cloud analysis via a bilateral local context augmentation structure and an adaptive feature fusion method; and (iv) refine the basic point feature representations with a plug-and-play module that incorporates both local context and global bilinear response to benefit various point cloud recognition problems and different baseline models. By conducting comprehensive experiments, ablation studies and visualizations, we quantitatively and qualitatively demonstrate our contributions in the deep learning-based research of 3D point clouds. In general, this thesis presents a review of deep learning-based 3D point cloud research, introduces our contributions in learning deep feature representations of point cloud data, and proposes research directions for future work. Apart from serving as a PhD work summary, we expect this thesis to inspire further exploration into 3D computer vision and its applications.
Secretory and viral fusion may share mechanistic events with fusion between curved lipid bilayers
Creators: name: Lentz, B. R.
name: Lee, J.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1998

description: Activation energies for the individual steps of secretory and viral fusion are reported to be large [Oberhauser, A. F., Monck, J. R. & Fernandez, J. M. (1992) Biophys. J. 61, 800–809; Clague, M. J., Schoch, C., Zech, L. & Blumenthal, R. (1990) Biochemistry 29, 1303–1308]. Understanding the cause for these large activation energies is crucial to defining the mechanisms of these two types of biological membrane fusion. We showed recently that the fusion of protein-free model lipid bilayers mimics the sequence of steps observed during secretory and viral fusion, suggesting that these processes may involve common lipid, rather than protein, rearrangements. To test for this possibility, we determined the activation energies for the three steps that we were able to distinguish as contributing to the fusion of protein-free model lipid bilayers. Activation energies for lipid rearrangements associated with formation of the reversible first intermediate, with conversion of this to a semi-stable second intermediate, and with irreversible fusion pore formation were 37 kcal/mol, 27 kcal/mol, and 22 kcal/mol, respectively. The first and last of these were comparable to the activation energies observed for membrane lipid exchange (42 kcal/mol) during viral fusion and for the rate of fusion pore opening during secretory granule release (23 kcal/mol). This striking similarity suggests strongly that the basic molecular processes involved in secretory and viral fusion involve a set of lipid molecule rearrangements that also are involved in model membrane fusion.
Stable alteration of pre-mRNA splicing patterns by modified U7 small nuclear RNAs
Creators: name: Kole, R.
name: Gorman, L.
name: Schumperli, D.
name: Suter, D.
name: Emerick, V.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1998

description: In several forms of β-thalassemia, mutations in the second intron of the β-globin gene create aberrant 5′ splice sites and activate a common cryptic 3′ splice site upstream. As a result, the thalassemic β-globin pre-mRNAs are spliced almost exclusively via the aberrant splice sites leading to a deficiency of correctly spliced β-globin mRNA and, consequently, β-globin. We have designed a series of vectors that express modified U7 snRNAs containing sequences antisense to either the aberrant 5′ or 3′ splice sites in the IVS2–705 thalassemic pre-mRNA. Transient expression of modified U7 snRNAs in a HeLa cell line stably expressing the IVS2–705 β-globin gene restored up to 65% of correct splicing in a sequence-specific and dose-dependent manner. Cell lines that stably coexpressed IVS2–705 pre-mRNA and appropriately modified U7 snRNA exhibited up to 55% of permanent restoration of correct splicing and expression of full-length β-globin protein. This novel approach provides a potential alternative to gene replacement therapies.
On the Role of Information Theoretic Uncertainty Relations in Quantum Theory
Creators: name: Jizba, Petr
name: Dunningham, Jacob A.
name: Joo, Jaewoo
Publisher: name: arXiv
Year: 2014
Subjects: subject: Quantum Physics (quant-ph)
subject: Mathematical Physics (math-ph)
subject: FOS: Physical sciences

description: Uncertainty relations based on information theory for both discrete and continuous distribution functions are briefly reviewed. We extend these results to account for (differential) Rényi entropy and its related entropy power. This allows us to find a new class of information-theoretic uncertainty relations (ITURs). The potency of such uncertainty relations in quantum mechanics is illustrated with a simple two-energy-level model where they outperform both the usual Robertson-Schrödinger uncertainty relation and Kraus-Maassen Shannon entropy based uncertainty relation. In the continuous case the ensuing entropy power uncertainty relations are discussed in the context of heavy tailed wave functions and Schrödinger cat states. Again, improvement over both the Robertson-Schrödinger uncertainty principle and Shannon ITUR is demonstrated in these cases. Further salient issues such as the proof of a generalized entropy power inequality and a geometric picture of information-theoretic uncertainty relations are also discussed.
description: 37 pages, 8 figures, RevTeX4
Suppression of cell transformation by the cyclin-dependent kinase inhibitor p57KIP2 requires binding to proliferating cell nuclear antigen
Creators: name: Pan, Z.-Q.
name: Xiong, Y.
name: Watanabe, H.
name: DePinho, R. A.
name: Hurwitz, J.
name: Schreiber-Agus, N.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1998

description: Proper control of the mammalian cell cycle requires the function of cyclin-dependent kinase (CDK) inhibitors. The p21 family currently includes three distinct genes, p21, p27Kip1, and p57Kip2, that share a common N-terminal domain for binding to and inhibiting the kinase activity of CDK-cyclin complexes. The p21 protein also binds to proliferating cell nuclear antigen (PCNA) through a separate C-terminal domain affecting DNA replication and repair. The p27 and p57 proteins also each contain unique C-terminal domains whose functions are unknown. Here we show that the human p57 protein, like p21, contains a PCNA-binding domain within its C terminus that, when separated from its N-terminal CDK-cyclin binding domain, can prevent DNA replication in vitro and S phase entry in vivo. Disruption of either CDK/cyclin or PCNA binding partially reduced p57’s ability to suppress myc/RAS-mediated transformation in primary cells, while loss of both inhibitory functions completely eliminated p57’s suppressive activity. Thus, control of cell cycle and suppression of cell transformation by p57 require both CDK and PCNA inhibitory activity, and disruption of either or both functions may lead to uncontrolled cell growth.
Bicultural Art: Offensive to the Māori or Acceptable Freedom of Expression? Wai 262, the CCPR and NZBORA
Creators: name: Lai, Jessica
Publisher: name: Open Access Te Herenga Waka-Victoria University of Wellington
Year: 2023
Subjects: subject: 160299 Criminology not elsewhere classified
subject: FOS: Law
subject: Sociology
subject: FOS: Sociology
subject: Law

description: No description supplied
Three Ancient Halo Subgiants: Precise Parallaxes, Compositions, Ages, and Implications for Globular Clusters
Creators: name: VandenBerg, Don A.
name: Bond, Howard E.
name: Nelan, Edmund P.
name: Nissen, P. E.
name: Schaefer, Gail H.
name: Harmer, Dianne
Publisher: name: arXiv
Year: 2014
Subjects: subject: Solar and Stellar Astrophysics (astro-ph.SR)
subject: FOS: Physical sciences

description: The most accurate ages for the oldest stars are those obtained for nearby halo subgiants, because they depend almost entirely on just the measured parallaxes and absolute oxygen abundances. In this study, we have used the Fine Guidance Sensors on the Hubble Space Telescope to determine trigonometric parallaxes, with precisions of 2.1% or better, for the Population II subgiants HD 84937, HD 132475, and HD 140283. High quality spectra have been used to derive their surface abundances of O, Fe, Mg, Si, and Ca, which are assumed to be 0.1-0.15 dex less than their initial abundances due to the effects of diffusion. Comparisons of isochrones with the three subgiants on the $(\log\,T_{\rm eff}, M_V)$-diagram yielded ages of $12.08 \pm 0.14, 12.56 \pm 0.46$, and $14.27 \pm 0.38$ Gyr for HD 84937, HD 132475, and HD 140283, in turn, where each error bar includes only the parallax uncertainty. The total uncertainty is estimated to be $\sim\pm 0.8$ Gyr (larger in the case of the near-turnoff star HD 84937). Although the age of HD 140283 is greater than the age of the universe as inferred from the cosmic microwave background by $\sim$ 0.4-0.5 Gyr, this discrepancy is at a level of $< 1\,σ$. Nevertheless, the first Population II stars apparently formed very soon after the Big Bang. (Stellar models that neglect diffusive processes seem to be ruled out as they would predict that HD 140283 is $\sim 1.5$ Gyr older than the universe.) The halo field subgiants appear to be older than globular clusters of similar metallicities: if distances close to those implied by the RR Lyrae standard candle are assumed, M92 and M5 are younger than HD 140283 and HD 132475 by $\sim 1.5$ and $\sim 1.0$ Gyr, respectively.
description: 55 pages, including 10 figures and 5 tables - accepted for publication in the Astrophysical Journal
Hyper-Skin: A Hyperspectral Dataset for Reconstructing Facial Skin-Spectra from RGB Images
Creators: name: Ng, Pai Chet
name: Chi, Zhixiang
name: Verdie, Yannick
name: Lu, Juwei
name: Plataniotis, Konstantinos N.
Publisher: name: Singapore Institute of Technology
Year: 2023

description: We introduce Hyper-Skin, a hyperspectral dataset covering wide range of wave-
lengths from visible (VIS) spectrum (400nm - 700nm) to near-infrared (NIR)
spectrum (700nm - 1000nm), uniquely designed to facilitate research on facial
skin-spectra reconstruction for consumer applications related to cosmetology and
skin well-being. By reconstructing skin spectra from RGB images, our dataset
enables the study of hyperspectral skin analysis, such as melanin and hemoglobin
concentrations, directly on the consumer device. Overcoming limitations of ex-
isting datasets, Hyper-Skin consists of diverse facial skin data collected with a
pushbroom hyperspectral camera. With 330 hyperspectral cubes from 51 subjects,
the dataset covers the facial skin from different angles and facial poses. Each
hyperspectral cube has dimensions of 1024×1024×448, resulting in millions of
spectra vectors per image. The dataset, carefully curated in adherence to ethical
guidelines, includes paired hyperspectral images and synthetic RGB images gen-
erated using real camera responses. We demonstrate the efficacy of our dataset
by showcasing skin spectra reconstruction using state-of-the-art models on 31
bands of hyperspectral data resampled in the VIS and NIR spectrums. This Hyper-
Skin dataset would be a valuable resource to NeurIPS community, encouraging
the development of novel algorithms for skin spectral reconstruction while fos-
tering interdisciplinary collaboration in hyperspectral skin analysis. Instructions
to request the data and the related benchmarking codes are publicly available at:
https://github.com/hyperspectral-skin/Hyper-Skin-2023.
The Arabidopsis thaliana RPM1 disease resistance gene product is a peripheral plasma membrane protein that is degraded coincident with the hypersensitive response
Creators: name: Nam, J.
name: Dangl, J. L.
name: Boyes, D. C.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1998

description: Disease resistance in plants is often controlled by a gene-for-gene mechanism in which avirulence (avr) gene products encoded by pathogens are specifically recognized, either directly or indirectly, by plant disease resistance (R) gene products. Members of the NBS-LRR class of R genes encode proteins containing a putative nucleotide binding site (NBS) and carboxyl-terminal leucine-rich repeats (LRRs). Generally, NBS-LRR proteins do not contain predicted transmembrane segments or signal peptides, suggesting they are soluble cytoplasmic proteins. RPM1 is an NBS-LRR protein from Arabidopsis thaliana that confers resistance to Pseudomonas syringae expressing either avrRpm1 or avrB. RPM1 protein was localized by using an epitope tag. In contrast to previous suggestions, RPM1 is a peripheral membrane protein that likely resides on the cytoplasmic face of the plasma membrane. Furthermore, RPM1 is degraded coincident with the onset of the hypersensitive response, suggesting a negative feedback loop controlling the extent of cell death and overall resistance response at the site of infection.
The domain organization of NaeI endonuclease: Separation of binding and catalysis
Creators: name: Colandene, J. D.
name: Topal, M. D.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1998

description: NaeI is a remarkable type II restriction endonuclease. It must bind two recognition sequences to cleave DNA, forms a covalent protein–DNA intermediate, and is only 1 aa change away from topoisomerase and recombinase activity. The latter activities apparently derive from reactivation of a cryptic DNA ligase active site. Here, we demonstrate that NaeI has two protease-resistant domains, involving approximately the N-terminal and C-terminal halves of the protein, linked by a protease-accessible region of 30 aa. The domains were purified by cloning. The C-terminal domain was shown by gel mobility-shift assay to have approximately 8-fold lower DNA-binding ability than intact NaeI. Analytical ultracentrifugation showed this domain to be a monomer in solution. The N-terminal domain, which contains the catalytic region defined by random mutagenesis, did not bind DNA and was a mixture of different-sized complexes in solution implying that it mediates self-association. DNA greatly inhibited proteolysis of the linker region. The results identify the DNA-binding domain, imply that DNA cleavage and recognition are independent and separable, and lead us to speculate about a cleft-like structure for NaeI.
The Regul8 Framework
Creators: name: Nursing Development Unit, Children's
name: Coetzee, Minette
Publisher: name: University of Cape Town
Year: 2023
Subjects: classificationCode: 321302
subject: Infant and child health

description: The Regul8 framework represents an approach to providing fundamental children’s nursing care. Developed by Associate Professor Minette Coetzee at the Harry Crossly Children’s Nursing Development Unit (Department of Paediatrics and Child Health, UCT), it was originally known as the Seven Steps, and then the Seven (Plus One) Steps. The impetus for the work was to align emerging understandings and research outcomes from the neuro sciences and human biology with current children’s nursing practice. Concepts were developed through continuous exploration of these emerging insights and refined through a decade of teaching and learning alongside children’s nursing students, practitioners and educators from across Africa. The framework is designed to comprehensively address the major influences on autonomic regulatory function through an intentionally Afrocentric guide to children’s nursing care planning.
The expression of matrix metalloproteinase 9 is enhanced by Epstein-Barr virus latent membrane protein 1
Creators: name: Pagano, J. S.
name: Furukawa, M.
name: Sato, H.
name: Yoshizaki, T.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1998

description: Matrix metalloproteinases (MMPs) are frequently expressed in malignant tumor cells and are thought to play crucial roles in tumor invasion and metastasis. Here we report that expression of MMP9 is increased in Epstein-Barr virus (EBV)-infected type III latency lymphoma cell lines, but not in type I lines where latent viral gene expression is highly restricted. Type III cell lines express abundant EBV latent membrane protein 1 (LMP1), the principal EBV oncoprotein, as well as the other latency proteins including the transcriptional factor, EBV nuclear antigen 2, which is also required for cell immortalization. Transfection of an LMP1 expression plasmid in the C33A cell line increased MMP9 expression, whereas overexpression of EBV nuclear antigen 2 did not. Three motifs, homologous to the binding sites of NF-κB, SP-1, and AP-1 proteins, contribute to induction of the MMP9 promoter by 12-O-tetradecanoyl-phorbol-13-acetate and tumor necrosis factor α. Here we report that binding sites for NF-κB, SP-1, and AP-1 also contribute to induction of the MMP9 promoter by the viral protein, LMP1, mainly through the NF-κB and, to a lesser extent, the SP-1 and AP-1 sites. Moreover the AP-1 binding site is essential in that mutation of it abolished reporter gene induction by LMP1. The enhancement of MMP9 expression was blocked by cotransfection of an IκB expression plasmid. Thus in addition to its transforming properties, the oncoprotein LMP1 may contribute to invasiveness and metastasis of EBV-associated tumors such as nasopharyngeal carcinoma.
D’Arcy v Myriad Genetics: A Demand for the “Made” or “Non-Information” and Clear Subject Matter?
Creators: name: Lai, Jessica
Publisher: name: Open Access Te Herenga Waka-Victoria University of Wellington
Year: 2023
Subjects: subject: Law
subject: FOS: Law

description: In October 2015, the High Court of Australia (HCA) handed down D’Arcy v. Myriad Genetics and overturned the Full Court of the Federal Court of Australia by holding that key product claims from Myriad Genetics’ BRCA1 gene patent did not constitute manners of manufacture. Two years earlier, the Supreme Court of the United States (SCOTUS) had similarly ruled against certain product claims from Myriad Genetics’ BRCA1 and BRCA2 patents, finding that simply isolated genetic sequences are not patentable subject matter. From their results, one could easily make the mistake of seeing the two decisions as being identical and placing Australia and the US at odds with Europe. However, as this article highlights, Australian law is conceptually different from US law and, strictly speaking, the HCA did not rule that isolated genetic sequences can never constitute patentable subject matter. However, at the end of the day, it is arguable that the laws are very similar in effect. This article examines the HCA decision and compares and contrasts it to that of SCOTUS.
Investigation of metal-insulator like transition through the ab initio density matrix renormalization group approach
Creators: name: Fertitta, E.
name: Paulus, B.
name: Barcza, G.
name: Legeza, Ö.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Strongly Correlated Electrons (cond-mat.str-el)
subject: Chemical Physics (physics.chem-ph)
subject: FOS: Physical sciences

description: We have studied the Metal-Insulator like Transition (MIT) in lithium and beryllium ring-shaped clusters through ab initio Density Matrix Renormalization Group (DMRG) method. Performing accurate calculations for different interatomic distances and using Quantum Information Theory (QIT) we investigated the changes occurring in the wavefunction between a metallic-like state and an insulating state built from free atoms. We also discuss entanglement and relevant excitations among the molecular orbitals in the Li and Be rings and show that the transition bond length can be detected using orbital entropy functions. Also, the effect of different orbital basis on the effectiveness of the DMRG procedure is analyzed comparing the convergence behavior.
description: 12 pages, 14 figures
New quantitative methods for the analysis of linear enamel hypoplasia (LEH) in bioarchaeology
Creators: name: Cares Henriquez, Alejandra
Publisher: name: The Australian National University
Year: 2023

description: Linear enamel hypoplasia (LEH) is the term used to describe dental developmental defects that occur following childhood exposure to systemic physiological stress. The identification and analysis of LEH is standard practice in bioarchaeological studies, as the occurrence of these defects can be utilised to measure the relative level of health of past populations, identify adaptive life strategies, reconstruct childhood patterns of illness, and produce life histories of individuals. Despite being studied in numerous populations over the last 300 years, the standards used to assess these hypoplastic defects still vary considerably in terms of the methods employed, the resolution at which they are examined, and the reporting of results. Technological advances over the last couple of decades has given rise to the development of more refined and objective methods that allow for a quantitative analysis of these stress events. However, quite often, archaeological samples are unsuitable to be examined using these methods due to significant levels of labial/buccal surface wear. This thesis aims to improve and add to the bioarchaeological toolkit used for the identification and analysis of LEH while demonstrating the benefits of extracting additional quantitative data to investigate multiple aspects of LEH. This thesis addresses the limitations and issues of existing approaches through the development of several new methods, and a new comprehensive approach that relies solely on objective high-resolution methods for the analysis of LEH. Moreover, it makes the case for the standardisation of reporting protocols to ensure the comparability of results between published studies. Additionally, this thesis aims to develop a software program that incorporates the newly developed objective approach, which automates the analytical process of these stress events, while ensuring that these methods are useful, usable, and accessible to any researcher wanting to undertake the analysis of LEH in archaeological samples. Finally, these methods and tools will be used to assess LEH in a large dental sample from a late Iron Age archaeological site in Southeast Asia. This will constitute the first-ever comprehensive microscopic examination of LEH for the region to rely on a complete set of objective methods for the identification, and assessment of ten different parameters, including frequency, prevalence, chronology, duration, and periodicity of defects. In doing so, this thesis will provide new insights into health trends in the region following the intensification of agriculture and period of early state development.