Metadata supporting data files on the relationship between dual endothelin receptor inhibition and T-DM1 efficacy in brain metastases 2 from HER2-positive breast cancer
Creators: name: Askoxylakis, Vasileios
name: Ferraro, Gino B.
name: Badeaux, Mark
name: Kodack, David P.
name: Kirst, Isabelle
name: Shankaraiah, Ram C.
name: Wong, Christina S.F.
name: Duda, Dan G.
name: Fukumura, Dai
name: Jain, Rakesh K.
Publisher: name: figshare
Year: 2019
Subjects: subject: Cancer
subject: 111203 Cancer Genetics
subject: FOS: Clinical medicine
subject: FOS: Clinical medicine
subject: 111205 Chemotherapy

description: This data record describes the data files that support the findings of the related publication: Dual endothelin receptor inhibition enhances T-DM1 efficacy in brain metastases from HER2-positive breast cancer.<br>The study investigates the effective treatment of cerebral metastases from HER2-positive breast cancer in terms of the influence of macitentan, a dual inhibitor of endothelin receptors A and B, on ado-trastuzumab emtansine (T-DM1) efficacy against breast cancers grown in the brain. T-DM1 is an anti-HER2 antibody-drug conjugate. The study also reports on the effect of the endothelin axis mediating protection of HER2-amplified brain cancers against T-DM1.<br><b>Study design summary:</b><br>The methodology producing these data involved treatment of mice bearing human HER2-amplified BT474 brain tumors, engineered to express secretable Gaussia luciferase (BT474-Gluc), with T-DM1 alone or in combination with macitentan. Mouse survival in days was then analysed.<br>The data files present both raw images and numerical data on survival rates and relative activity or Gaussia luciferase in BT474-Gluc cells.<br><b>Format of data and number of files:</b>8 graph files in <b>.pzfx</b> image format6 Western blot raw image files in <b>.pdf </b>format2 representative raw images in <b>.tiff </b>image format1 data table in <b>.xlsx</b> format<b><br></b><b>Data files and sample sizes:</b>The data files supporting the figures of the related publication are listed below.<b><br></b><b>Software needed to access data:</b><br>Data files are all accessible via openly available image viewing software or Microsoft Excel/open office applications.<br><b>Mouse_Survival.pzfx </b>(Figure 1a) - Mouse survival for control and macitentan groups (N=18-21). % survival vs days after treatment initiation.<b>Apoptosis_Analysis.pzfx </b>(Figure 1c) - Cell apoptosis. % potential positive cells vs treatment group (N=5-9).<b>Apoptosis_Imaging.tiff </b>(Figure 1d) - BT474-Gluc tumor images in the brain after staining for ApopTag<b>Slice_Culture_Growth.pzfx</b> (Figure 1e) - Relative activity of Gaussia luciferase in BT474-Gluc cells. Gaussia luciferase fold change vs days after treatment initiation. (N=5-7)<b>W</b><b>estern_Blot.pdf (</b>Figure 1b), <b>Western_Blot.pdf (</b>Figure 1f), <b>Western_Blot.pdf (</b>Figure 1g), <b>Western_Blot.pdf (</b>Figure 1h). - Western blot image files for BT474-Gluc brain tumors and treatment groups.<br><b>Slice_Culture_Imaging.tiff </b>(Supplementary figure 1a) - confocal organotypic brain slice image <b>Slice_Culture_Growth.pzfx </b>(Supplementary figure 1b), <b>Slice_Culture_Growth.pzfx </b>(Supplementary figure 1c)- Relative activity of Gaussia luciferase in the media of BT474-Gluc cells. Gaussia luciferase fold change vs days post treatment.<b>Western_Blot_Band_Intensity.xlsx </b>(Supplementary figure 1e) - fold change in control groups for western bands (N=2)<b>Mouse_Growth.pzfx </b>(Supplementary figure 1f), <b>Mouse_Survival.pzfx </b>(Supplementary figure 1g)- tumor growth and mouse survival. Gaussia luciferase and % survival (respectively) vs days after treatment initiation (N=3-5)<b>Mouse_Survival.pzfx </b>(Supplementary figure 1h) - Survival of mice bearing HER2-amplified PIK3CA mutant (E545K) MDA-MB-361-Gluc tumors (N=6).<b>Western_Blot.pdf </b>(Supplementary figure 1d)<b>, Western_Blot.pdf </b>(Supplementary figure 1i) - Western blot image files for BT474-Gluc brain tumors and treatment groups.<br>See figure captions in the related publication for more detailed information on these files.<br><b>Data access: </b><br>All data files are stored in Institutional file storage at Edwin L. Steele Laboratories/Massachusetts General Hospital and are available on request from the Dr Jain at the following email: PAtoDrJain@steele.mgh.harvard.edu