<b>Supplementary information for the manuscript titled "Analysis of Fruit and Root Movement in Two Orchid Species Using 2D Photographs and a Bioinformatics Pipeline for Sequential 3D Scans Processing"</b>
Creators: name: Pramanik, Dewi
name: Maaria Vaskimo, Lotta
name: Batenburg, K. Joost
name: Kostenko, Alexander
name: Droppert, Kevin
name: Smets, Erik
name: Gravendeel, Barbara
Publisher: name: figshare
Year: 2023
Subjects: classificationCode: 310404
subject: Evolution of developmental systems

description: The Supporting Information comprises 11 appendices, which include:<b>Appendix S1.</b> CT scan protocol of young, mature, early, and late flowering plants of <i>Erycina pusilla.</i><b>Appendix S2.</b> 3D CT scan contrast test of different media in which <i>Erycina pusilla</i> was grown: (<b>A</b>) gelrite; <b>B</b>) glass beads; <b>C</b>) sand; <b>D</b>) solid agar; <b>E</b>) <i>S</i><i>phagnum</i> moss; and <b>F</b>) cotton). <i>Scale</i><i> </i><i>bar: 1 cm.</i><b>Appendix S3</b>. Scanning and reconstructing images of <i>Erycina pusilla</i> plants with SkyScan1172 Micro CT: a step-by-step guide.<b>Appendix S4.</b> Response of five plants exposed to weekly X-ray radiation compared to four control plants not exposed to X-rays<i>.</i><b>Appendix S5.</b> Effect of repeated exposure to X-rays during CT-scanning over 19 weeks on development of young, mature, early, and fully flowering plants of <i>Erycina pusilla</i> compared with control plants not exposed to any X-rays.<b>Appendix S6.</b> Development of <i>Erycina pusilla</i><i> </i>from seed to flowering stage recorded in the tissue cultivation laboratory of Naturalis Biodiversity Center. <b>A</b>) Leaf-like protocorm visible 28 days after germination (DAG); <b>B</b>) First development of roots at 35 DAG; <b>C-G</b>) seedling development 42-70 DAG; <b>H-I</b>) Vegetative plant stages 77-84.DAG; <b>J-K</b>) inflorescence development and initiation of resupination ± 180 degrees of the flower 91-98 DAG; <b>L-M)</b> Elongation of inflorescence stalk 105-112 DAG; <b>O</b>) Floral bud emerging from bract 119 DAG; <b>P-Q</b>) 30 degrees bending of pedicel until (<b>R-S</b>) flower opens 126-133 DAG. <i>Scale bar: 1 cm.</i><b>Appendix S7.</b><b> </b>Resupination of roots of <i>Erycina pusilla</i> after different exposure times to x-rays. <i>Abbreviations: Root S-1: root of seedling; Root NF1-1: root of the first replication non-flowering plant; Root NF2-1= root number one of second replication of non-flowering plant; Root NF2-2= root number two of second replication of non-flowering plant; Root NF2-3= root number three of second replication of non-flowering plan</i><i>ts.</i><b>Appendix S8.</b> Cell number data of <i>P. equestris</i> fruits analyzed.<b>Appendix S9.</b><b> </b>Area measurement data of <i>P. equestris</i> fruits analyzed.<b>Appendix S10.</b><b> </b>Number of cells of each valve in eight developmental stages of <i>Phalaenopsis equestris</i><i> </i>fruits grown in the tissue cultivation laboratory of Naturalis Biodiversity Center. The number of cells is shown on the vertical axis, and the fruit developmental stages (days after pollination-DAP) are displayed on the horizontal axis. <i>Abbreviations: Lab= fertile valve derived from labellum; P1= fertile valve derived from the first petal; P2= fertile valve derived from the second petal; S1= sterile valve originated from first lateral sepal; S2= sterile valve originated from dorsal sepal, and S3= sterile valve originated from second lateral sepal. Error bars are indicated in black.</i><b>Appendix S11</b>. Main morphological changes of <i>Phalaenopsis equestris</i> fruits (N=3) observed during development. <i>Abbreviations DAP= days after pollination.</i><br><br><br>