Additional file 1 of Sterilization protocols and the effect of plant growth regulators on callus induction and secondary metabolites production in in vitro cultures Melia azedarach L.
Creators: name: Ahmadpoor, Fatemeh
name: Zare, Nasser
name: Asghari, Rasool
name: Sheikhzadeh, Parisa
Publisher: name: figshare
Year: 2022
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Biotechnology
subject: Ecology
subject: Science Policy
subject: 69999 Biological Sciences not elsewhere classified
subject: Inorganic Chemistry
subject: FOS: Chemical sciences
subject: 111714 Mental Health
subject: FOS: Health sciences
subject: Plant Biology

description: Additional file 1: Figure S1. In vitro culture of M. azedarach L.; A1: leaf explants, B1: viable and browning leaf explants after disinfection and in vitro culture, C1: swollen leaf explants and initiation of callus induction on MS medium containing 1 mg/L NAA + 1 mg/L Kin, D1 and E1: callus induced on MS medium containing 1 mg/L NAA + 1 mg/L BAP and 3 mg/L NAA + 1 mg/L BAP (is embryogenic callus), respectively, A2 and B2: callus initiation and growth on MS + 1 mg/L 2,4-D + 1 mg/L Kin, C2, D2 and E2: callus growth on MS medium containing 1 mg/L 2,4-D + 1 mg/L Kin, 1 mg/L NAA + 1 mg/L Kin and 3 mg/L 2,4-D + 3 mg/L BAP, respectively. Figure S2. In vitro culture of M. azedarach L.; A1: callus induction on MS medium containing 3 mg/L NAA + 1 mg/L Kin containing green cells in callus and B1: 3 mg/L NAA + 1 mg/L BAP containing green and globular shape spots in callus (are embryogenic callus), C1: embryogenic callus that have taken root, A2: globular and torpedo shape embryos, B2: globular embryos under a stereoscope and C2: torpedo shape embryos under a stereoscope. Figure S3. In vitro culture of M. azedarach L.; Callus growth on MS + A1: 1 mg/L NAA + 1 mg/L Kin, B1: 1 mg/L NAA + 1 mg/L BAP, C1: 1 mg/L 2,4-D + 1 mg/L Kin, D1: 1 mg/L 2,4-D + 1 mg/L BAP, A2: 3 mg/L NAA + 1 mg/L Kin, B2: 3 mg/L NAA + 1 mg/L BAP, C2: 3 mg/L NAA + 3 mg/L Kin, D2: 3 mg/L NAA + 3 mg/L BAP, A3: 3 mg/L 2,4-D + 1 mg/L Kin, B3: 3 mg/L 2,4-D + 1 mg/L BAP, C3: 3 mg/L 2,4-D + 3 mg/L Kin, D3: 3 mg/L 2,4-D + 3 mg/L BAP, A4: 5 mg/L NAA + 1 mg/L Kin, B4: 5 mg/L NAA + 1 mg/L BAP, C4: 5 mg/L NAA + 5 mg/L Kin, D4: 5 mg/L NAA + 5 mg/L BAP, A5: 5 mg/L 2,4-D + 1 mg/L Kin, B5: 5 mg/L 2,4-D + 1 mg/L BAP, C5: 5 mg/L 2,4-D + 5 mg/L Kin, D5: 5 mg/L 2,4-D + 5 mg/L BAP, respectively. Figure S4. HPLC chromatogram of rutin, quercetin and kaempferol in the standard mixture (A) and M. azedarach L. calli grown on the MS medium containing 3 mg/L NAA + 3 mg/L Kin (B). Table S1. Different disinfection methods used in experiment 1 for sterilization of M. azedarach L. leaf explants. Table S2. Different disinfection methods and inclusion of benomyl in culture medium used in experiment 2 for sterilization of M. azedarach L. leaf explants. Table S3. The effect of different disinfection methods on disinfection indices of leaf explants in M. azedarach L. (Experiment 1). Table S4. Effect of PGRs treatment on total flavonoid, total phenol and anthocyanin contents in M. azedarach L. calli. Table S5. Effect of PGRs treatment on rutin, quercetin and kaempferol in M. azedarach L. by HPLC analysis.