cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
title: Balancing Act: An Exploration into the Wellbeing of Bisexual+ People in Monogamous Relationships
Creators: name: Byrne, Sakura Persephone Louise
Publisher: name: The Open University
Year: 2024

description: Research has repeatedly documented high rates of mental distress among bisexual+ individuals. Moreover, bisexuality+ has continually been conceptualised in a plethora of negative ways, and Western views of gender and sexuality as dualistic in nature has led to the assumption that bisexual+ people are non-monogamous by necessity. Bisexual+ individuals are therefore conceptualised as always behaviourally/actively bisexual+. However, many bisexual+ individuals are engaged in monogamous relationships, yet their experiences have been under-researched. This thesis seeks to elucidate the experiences of bisexual+ people in monogamous relationships, and to explore how these experiences impact upon their wellbeing. Using a multi-modal methodological approach, the ways in which participants navigate their relationships whilst managing their wellbeing is both discursively and materially explored.

Fieldwork consisted of a focus group with six participants, and individual interviews with a further 25 participants. Thematic analysis of the focus group data was used to identify key areas of importance in the lived experience of monogamous bisexual+ people. The results of this analysis were used to develop the open-ended interview schedule. These interviews used photo-elicitation, thereby allowing participants to construct a narrative that incorporated their materialities. Phenomenologically-informed discourse analysis was used to interpret the interview data.

The results demonstrate that bisexual+ people in monogamous relationships undertake a complex balancing act in relation to their wellbeing. Participants were able to create spaces and networks within which they experienced themselves as embodied bisexual+ subjects, with their relationships often acting as safe havens that bolstered their wellbeing. However, tensions within participant discourses revealed experiences of discrimination and identity abuse within romantic relationships, which negatively impacted their wellbeing. Participants expressed the desire to incorporate their bisexuality+ into their materiality, but this was frequently invisibilised by the presence of a differently-gendered partner and by a lack of a clear understanding of bisexuality+ within society.
Culicoides teretipalpis Wirth & Barreto 1978
Creators: name: Spinelli, Gustavo
name: Wolff, Marta
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Ceratopogonidae
Culicoides
Culicoides teretipalpis

description: teretipalpis Wirth & Barreto, 1978: 557. Type locality: Colombia, Valle del Cauca (Río Raposo). HT F (# 71156 USNM). Distr.: Colombia (Antioquia (Río Anorí), Valle del Cauca (Río Raposo)). Refs.: Wirth & Barreto, 1978: 557; Barreto, 1986: 148; Wirth et al., 1988: 38; Borkent & Spinelli, 2007: 71; Spinelli et al., 2009: 89; Borkent, 2015: 104 (cat.).
description: Published as part of Spinelli, Gustavo & Wolff, Marta, 2016, FAMILY CERATOPOGONIDAE, pp. 98-141 in Zootaxa 4122 (1) on page 115, DOI: 10.11646/zootaxa.4122.1.15, http://zenodo.org/record/256311
CORPOREALITY AND ITS IMPACT ON INCLUSIVE EDUCATIONAL PRACTICES
Creators: name: Giovanni Paolo, Rizzo
Publisher: name: TOP Journal
Year: 2024
Subjects: Academic achievement; Physical education; Pedagogy; Special pedagogy.

description: Physical activity is an important tool for integration and inclusion that leads to respect for diversity because the need to move is also fundamental in the person with disabilities as it expresses their physical, emotional, mental, and social potential that emerges in the dimension in which the subject is in tune with himself, the family, the community and the world in general. Motor activities represent a privileged meeting point of diversity, which is thus included without distinction using the abilities of each one. The heterogeneity of students with special educational needs requires the implementation of a variety of responses that, by combining good didactic/educational planning with innovative pedagogical devices, are able to carry out individualized and personalized interventions by enhancing the resources of the school community. The present work, focusing on corporeality as a cognitive device, opens a reflection on the way in which to operationalize the educational process by initiating and implementing new personalized teaching protocols.
Direct numerical simulation and a new 3-D discrete dynamical system for image-based complex flows using volumetric lattice Boltzmann method
Creators: name: Zhang, Xiaoyu
Publisher: name: Purdue University Graduate School
Year: 2024
Subjects: classificationCode: 401706
subject: Numerical modelling and mechanical characterisation

description: The kinetic-based lattice Boltzmann method (LBM) is a specialized computational fluid dynamics (CFD) technique that resolves intricate flow phenomena at the mesoscale level. The LBM is particularly suited for large-scale parallel computing on Graphic Processing Units (GPU) and simulating multi-phase flows. By incorporating a volume fraction parameter, LBM becomes a volumetric lattice Boltzmann method (VLBM), leading to advantages such as easy handling of complex geometries with/without movement. These capabilities render VLBM an effective tool for modeling various complex flows. In this study, we investigated the computational modeling of complex flows using VLBM, focusing particularly on pulsatile flows, the transition to turbulent flows, and pore-scale porous media flows. Furthermore, a new discrete dynamical system (DDS) is derived and validated for potential integration into large eddy simulations (LES) aimed at enhancing modeling for turbulent and pulsatile flows. Pulsatile flows are prevalent in nature, engineering, and the human body. Understanding these flows is crucial in research areas such as biomedical engineering and cardiovascular studies. However, the characteristics of oscillatory, variability in Reynolds number (Re), and shear stress bring difficulties in the numerical modeling of pulsatile flows. To analyze and understand the shear stress variability in pulsatile flows, we first developed a unique computational method using VLBM to quantify four-dimensional (4-D) wall stresses in image-based pulsatile flows. The method is validated against analytical solutions and experimental data, showing good agreement. Additionally, an application study is presented for the non-invasive quantification of 4-D hemodynamics in human carotid and vertebral arteries. Secondly, the transition to turbulent flows is studied as it plays an important role in the understanding of pulsatile flows since the flow can shift from laminar to transient and then to turbulent within a single flow cycle. We conducted direct numerical simulations (DNS) using VLBM in a three-dimensional (3-D) pipe and investigated the flow at Re ranging from 226 to 14066 in the Lagrangian description. Results demonstrate good agreement with analytical solutions for laminar flows and with open data for turbulent flows. Key observations include the disappearance of parabolic velocity profiles when Re>2300, the fluctuation of turbulent kinetic energy (TKE) between laminar and turbulent states within the range 2300
Toxic Stereotypes: An Analysis of Selected Media Distortions Using Stuart Hall's Model
Creators: name: Essien, Essien
Publisher: name: Zenodo
Year: 2024

description: Media plays a significant role in shaping societal perceptions and beliefs influencing the way individuals view themselves and others. Stuart Hall's communication model highlights the importance of media in constructing meaning, showing that representations in media and other cultural texts are not neutral or objective, but rather are shaped by dominant discourses and power structures. In the light of this perspective, this study aims to analyze viral and harmful stereotypes perpetuated by media representations, to examine the toxic impact of these stereotypes on individuals and society and to evaluate the role of media in shaping cultural perceptions within the framework of Hall's model. The research considers the stereotypes at Three Levels (3Ls); Toxic Masculinity (TM), Racial and Ethnic Stereotypes (RETs), as well as Gender and Sexual Stereotypes (GSSs). The Desk Research Method is employed to review existing studies and publications on such harmful media stereotypes that tend to align with Hall's postulations, towards extracting and framing these biases for critical discourses. This research is significant as it contributes to the existing body of knowledge on media effects and representation shedding light on the detrimental impact of harmful stereotypes on historical, cultural and sexual identities as perpetuated by the media. By uncovering the ills of media-driven stereotypes which mis-shapes societal attitudes and perceptions, this study aims to raise awareness on the need for critical media literacy among media consumers.
Forcipomyia belemensis Wirth & Blanton 1973
Creators: name: Spinelli, Gustavo
name: Wolff, Marta
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Ceratopogonidae
Forcipomyia
Forcipomyia belemensis

description: belemensis Wirth & Blanton, 1973: 427. Type locality: Brazil, Pará, Belém. HT F (USNM). Distr.: Colombia (?), Brazil (Amazonas, Pará). Refs.: Wirth & Blanton, 1973: 427; Wirth et al., 1988: 32; Borkent & Spinelli, 2007: 71; Spinelli et al., 2009: 89.
description: Published as part of Spinelli, Gustavo & Wolff, Marta, 2016, FAMILY CERATOPOGONIDAE, pp. 98-141 in Zootaxa 4122 (1) on page 115, DOI: 10.11646/zootaxa.4122.1.15, http://zenodo.org/record/256311
title: Mechanismen der Zellmigration bei Entzündungen
Creators: name: Ishikawa-Ankerhold, Hellen
Publisher: name: Ludwig-Maximilians-Universität München
Year: 2024
Subjects: subject: cell migration, cytoskeleton proteins, intravital microscopy, multiphoton microscopy, leukocyte trafficking, inflammation, infection, cancer metastasis
subject: 600
subject: 610
subject: FOS: Medical and Health Sciences
valueUri: http://www.oecd.org/science/inno/38235147.pdf

description: Zellmigration ist in vielen Phasen der Entwicklung und des Erwachsenenlebens ein zentraler Prozess. Während einer Entzündung wandern Immunzellen geleitet von chemotaktischen Signalen zum Ort der Verletzung oder Infektion. Zellen können entweder einzeln wandern oder sich im Kontext von Geweben bewegen. Die Bewegung wird durch interne und externe Signale gesteuert, welche komplexe Signaltransduktionskaskaden aktivieren. Diese resultieren in einer hochdynamischen und lokalisierten Umgestaltung des Zytoskeletts und führen zu Zell-Zell- und Zell-Substrat-Interaktionen. Um diese Prozesse zu verstehen, ist es notwendig, die kritischen strukturellen Komponenten des Zytoskeletts, ihre räumlich-zeitliche Dynamik und ihre regulatorischen Signalwege zu identifizieren. Bei der Analyse dieser räumlich-zeitlichen Dynamik spielt die mikroskopische Bildgebung eine immer wichtigere und wirkungsvollere Rolle. In diesem Projekt wurden verschiedene Modellorganismen wie Dictyostelium discoideum und Mausmodelle in Kombination mit mikroskopischen Bildgebungstechniken verwendet und neu etabliert, um die verschiedenen Aspekte der Zellmotilität inklusive der Charakterisierung von Chemotaxis und Zytoskelettdynamik von Immunzellen während einer Entzündung zu untersuchen.
description: Cell migration is an essential process during many stages of development and adult life. During inflammation immune cells migrated to the site of injury or infection following chemotaxis signals. Cell movement is controlled by internal and external signals, which activate complex signal transduction cascades resulting in highly dynamic and localized remodeling of the cytoskeleton, cell-cell and cell-substrate interactions. To understand these processes, it is necessary to identify the critical structural cytoskeletal components, their spatio-temporal dynamics and their regulatory signaling pathways. Microscopic imaging plays an increasingly important and powerful role in the analysis of these spatio-temporal changes over time. In this project a variety of model organisms as Dictyostelium discoideum and mouse models combined with advanced microscopic imaging techniques have been used to investigate various aspects of cell motility including chemotaxis and cytoskeleton dynamics during inflammation.
Culicoides camposi Ortiz & Leon 1955
Creators: name: Spinelli, Gustavo
name: Wolff, Marta
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Ceratopogonidae
Culicoides
Culicoides camposi

description: camposi Ortíz & León, 1955: 580. Type locality: Ecuador, Pichincha, San Miguel de los Colorados, Zapallo Grande. HT F (UNCE). Distr.: Costa Rica, Panama, Colombia (Putumayo (Puerto Leguizamo), Valle del Cauca (Río Raposo)), Ecuador. Refs.: Ortíz & León, 1955: 580; Wirth & Blanton, 1973: 428; Barreto, 1986: 145; Wirth et al., 1988: 32; Borkent & Spinelli, 2007: 71; Spinelli et al., 2009: 89.
fairchildi Wirth & Blanton, 1955a: 102. Type locality: Panama, Canal Zone, Madden Dam. HT F (# 62801 USNM).
description: Published as part of Spinelli, Gustavo & Wolff, Marta, 2016, FAMILY CERATOPOGONIDAE, pp. 98-141 in Zootaxa 4122 (1) on page 115, DOI: 10.11646/zootaxa.4122.1.15, http://zenodo.org/record/256311
title: The Effects of Post-Outcome Information on Hindsight Bias
Creators: name: Whittet, Brad
Publisher: name: Old Dominion University
publisherIdentifier: https://ror.org/04zjtrb98
publisherIdentifierScheme: ROR
Year: 2009

description: The Hindsight Bias Effect describes the observation that people's retrospective judgments are systematically more in favor of the given outcome than their predictive judgments. Over the last 30 years, the Hindsight Bias has been one of the most researched judgment phenomena and a number of competing theories have arisen to explain the effect. This study investigated the effect of post-outcome information on the size and direction of the hindsight bias. In order to test the predictions of different theories of hindsight bias, two aspects of post-outcome information were manipulated: I) source (internal vs. external), and 2.) congruency with the outcome information. Findings from the study indicate evidence for a sense-making model as being a strong candidate for accurately explaining hindsight biases, as well as uncovering a new phenomenon of negative hindsight bias in specified conditions.
Culicoides beaveri Wirth & Barreto 1978
Creators: name: Spinelli, Gustavo
name: Wolff, Marta
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Ceratopogonidae
Culicoides
Culicoides beaveri

description: beaveri Wirth & Barreto, 1978: 557. Type locality: Colombia, Valle del Cauca (Río Raposo). HT F (# 71155 USNM). Distr.: Colombia (Valle del Cauca (Río Raposo)). Refs.: Wirth & Barreto, 1978: 557; Barreto, 1986: 145; Wirth et al., 1988: 28; Borkent & Spinelli, 2007: 71; Spinelli et al., 2009: 89.
description: Published as part of Spinelli, Gustavo & Wolff, Marta, 2016, FAMILY CERATOPOGONIDAE, pp. 98-141 in Zootaxa 4122 (1) on page 115, DOI: 10.11646/zootaxa.4122.1.15, http://zenodo.org/record/256311
title: Osteoinductivity of Demineralized Bone: A Quantitative In Vitro Assessment
Creators: name: Zheng, Yihong
Publisher: name: Old Dominion University
publisherIdentifier: https://ror.org/04zjtrb98
publisherIdentifierScheme: ROR
Year: 1991

description: Acid demineralized bone (DMB) implanted in extraosseous sites induces bone formation. In vivo studies have shown that DMB stimulates the differentiation of mesenchymal cells into osteogenic cells. In this study an in vitro system was developed to demonstrate the osteoinductivity of DMB quantitatively. This in vitro working system was used to assess the osteoinductivity of variously processed DMB products as a means to evaluate their processing protocols. A human dermal fibroblastic (HDF) cell line was initiated from tissue explants as a working model, along with a human periosteal (HPO) cell line as a control. Ground DMB processed by LifeNet Transplant Services was added to HDF cell cultures and changes in cell growth and functional characteristics were monitored over a 7-day incubation period. Cell proliferation rate decreased, as indicated by decreased total cell number and decreased rate of [3H]-thymidine incorporation. Cell size decreased, using the amount of protein content per cell as a measure. Alkaline phosphatase (AP) activity also increased. These characteristics of stimulated HDF cells listed above are more typical of HPO cells rather than HDF cells used as a control, suggesting that DMB had stimulated HDF cells to display phenotypic characteristics typical of osteogenic cells.

AP activity was chosen as a quantitative measure for osteoinductivity of various kinds of DMB products because it has been reported to be an exclusive indicator of osteoinducing cells and has been widely used as a marker for osteogenic cells. AP activities of HDF cells showed no significant difference when cultured with demineralized bone and ethanol-pretreated demineralized bone, indicating that ethanol pretreatment does not affect the osteoinductive potential of DMB.
Culicoides daedaloides Wirth & Blanton 1959
Creators: name: Spinelli, Gustavo
name: Wolff, Marta
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Ceratopogonidae
Culicoides
Culicoides daedaloides

description: daedaloides Wirth & Blanton, 1959: 330. Type locality: Panama, Bocas del Toro, Amirante. HT F (# 63163 USNM). Distr.: Panama, Colombia (Valle del Cauca (Río Raposo)), northwestern Argentina. Refs.: Wirth & Blanton, 1959: 330; Barreto, 1986: 148; Borkent & Spinelli, 2007: 71; Spinelli et al., 2009: 89.
description: Published as part of Spinelli, Gustavo & Wolff, Marta, 2016, FAMILY CERATOPOGONIDAE, pp. 98-141 in Zootaxa 4122 (1) on page 115, DOI: 10.11646/zootaxa.4122.1.15, http://zenodo.org/record/256311
CF in the context of developments in the Research Data Alliance (RDA)
Creators: name: Stockhause, Martina
Publisher: name: Zenodo
Year: 2024
Subjects: RDA
CF
Climate and Forecast
Climateurope2
2024 CF Workshop

description: Presentation at the CF (Climate and Forecast) Workshop 2024 at SMHI, Norrköping, 17-20 September 2024 on developments within the Research Data Alliance (RDA), which are of interst for the CF community.
Animalia
Creators: name: Spinelli, Gustavo
name: Wolff, Marta
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia

description: dasyophrus Macfie, 1940c: 27. Type locality: Guyana, New River. ST M, F (BMNH). Distr.: Colombia (Meta (Refugio Macarena)), Ecuador, Venezuela, Guyana, Brazil (Amazonas, Mato Grosso, Pará). Refs.: Macfie, 1940c: 27; Wirth & Blanton, 1973: 430; Barreto, 1986: 146; Wirth et al., 1988: 50; Borkent & Spinelli, 2007: 72; Spinelli et al., 2009: 89.
description: Published as part of Spinelli, Gustavo & Wolff, Marta, 2016, FAMILY CERATOPOGONIDAE, pp. 98-141 in Zootaxa 4122 (1) on page 115, DOI: 10.11646/zootaxa.4122.1.15, http://zenodo.org/record/256311
GENDERACTIONplus Position Paper 5: Gender-based Violence in the ERA: An Endemic Problem in Need of a New Cure
Creators: name: GENDERACTIONplus
Publisher: name: Zenodo
Year: 2024
Subjects: subject: Gender-Based Violence
subject: ERA

description: Nearly two in three (62%) of the over 42,000 respondents in the UniSAFE survey on gender-based violence in research organisations stated that they had experienced at least one form of gender-based violence within their institution (including psychological, physical, sexual, economic, and online forms). Respondents from minority groups reported even higher rates of gender-based violence (UniSAFE 2022). These findings have been confirmed in other studies in EU national contexts (HEA 2021; Rudolfsson et al 2022) as well as in research (Bondestam & Lundqvist 2020). Gender-based violence has severe consequences for individuals, including stress, depression, anxiety, alcohol abuse, lack of motivation, an increased tendency to interrupt studies or leave work, and deteriorating mental and physical health, and it also impedes participation and affects perceptions of safety in the study and work environment in general (McDonald 2012; Selkie et al 2015).by RCN in WP8. The amount will cover additional approx. 4.5 PM at ISAS CR.
Animalia
Creators: name: Spinelli, Gustavo
name: Wolff, Marta
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia

description: crescentis Wirth & Blanton, 1959: 317. Type locality: Panama, Canal Zone, Mojinga Swamp. HT F (# 63159 USNM). Distr.: Mexico (Chiapas) to Colombia (?), northeastern Argentina. Refs.: Wirth & Blanton, 1959: 317; Wirth et al., 1988: 28; Borkent & Spinelli, 2007: 71; Spinelli et al., 2009: 89.
description: Published as part of Spinelli, Gustavo & Wolff, Marta, 2016, FAMILY CERATOPOGONIDAE, pp. 98-141 in Zootaxa 4122 (1) on page 115, DOI: 10.11646/zootaxa.4122.1.15, http://zenodo.org/record/256311
Culicoides daedalus Macfie 1948
Creators: name: Spinelli, Gustavo
name: Wolff, Marta
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Ceratopogonidae
Culicoides
Culicoides daedalus

description: daedalus Macfie, 1948: 83. Type locality, Mexico, Chiapas, El Vergel. HT M (BMNH). Distr.: USA (Arizona, New Mexico), Mexico (Chiapas) to Colombia (?). Refs.: Macfie, 1948: 83; Wirth et al., 1988: 28; Borkent & Spinelli, 2007: 71; Spinelli et al., 2009: 89.
description: Published as part of Spinelli, Gustavo & Wolff, Marta, 2016, FAMILY CERATOPOGONIDAE, pp. 98-141 in Zootaxa 4122 (1) on page 115, DOI: 10.11646/zootaxa.4122.1.15, http://zenodo.org/record/256311
THE POWER SUPPLY OF RAILWAY ENTERPRISE
Creators: name: Abdullayeva Rukhsora
Publisher: name: Zenodo
Year: 2024

description: Annotation: Despite some peculiarities, power supply to non-tradable railway transport consumers is carried out on the same principles as industrial power supply. Due to significant moral and physical wear and tear of the main production equipment and its electrical networks and systems, a number of enterprises are undergoing a comprehensive reconstruction of the entire production process. At the same time, it becomes possible to revise the power supply system in a new way, re-optimize the configuration of the supply and distribution network, and apply modern samples of electrical equipment.

Keywords: power supply, labor protection, technological processes, electrical equipment
Services und Strukturen für ein standardisiertes Forschungsdatenmanagement (FDM) an der HTW Berlin
Creators: name: Schneidenbach, Esther
name: Heister, Eske Carmen
name: Dąbrowska, Paulina Anna
Publisher: name: Zenodo
Year: 2024
Subjects: FitForFDM
FDM@HAW
FDM
Forschungsdatenmanagement
Hochschule für angewandte Wissenschaften
Fachhochschule
HAW
FH
Datenkompetenzen
FDM-Kompetenzen an HAW 2024

description: Das Poster veranschaulicht die im Rahmen des Projekts FitForFDM an der HTW Berlin entwickelten und implementierten Services und Strukturen für ein standardisiertes Forschungsdatenmanagement (FDM). Hierzu zählt die Entwicklung einer zentralen FDM-Plattform (FDM One-Stop-Shop) für Hochschuleangehörige mit allgemeinen Informationen und Hilfestellungen zum FDM verknüpft mit Hinweisen auf an der Hochschule vorhanden Kompetenzen und Ressourcen. Weiterhin wird auf die entwickelten Unterstützungsmaterialien auf Moodle (Formulare zur Erstellung eines Datenmanagementplans und zur Datendokumentation in Form von README-Dateien) sowie eine Forschungsdaten-Policy, FDM-Strategie, Schulungsmaterialien, ein Mapping der vorhandenen Angebote und Ressourcen z. B. in Form einer Speichergrafik verwiesen. Das Poster dient dazu, die im Projekt erfolgten Erkenntnisse und Ressourcen mit anderen Hochschulen für angewandte Wissenschaften (HAWs) zu teilen und sich im Aufbau von FDM-Strukturen auszutauschen und zu unterstützen.

Dieses Poster wurde im Rahmen der VW-Themenwoche "Digitale Kompetenzen in der Wissenschaft" im Workshop "FDM mit geringen Ressourcen. Kompetenzaufbau durch Vernetzung von Hochschulen für angewandte Wissenschaften" am 02. Dezember 2024 vorgestellt.
AI-PROGNOSIS Newsletter No 3
Creators: name: Leipuvienė, Kristina
Publisher: name: Zenodo
Year: 2024
Subjects: Parkinson's disease
Parkinson's
Digital health solutions
Clinical studies
Artificial Intelligence
Digital biomarkers

description: We kindly welcome you to get acquainted with AI-PROGNOSIS - Horizon Europe research and innovation project aiming to advance Parkinson’s disease diagnosis and care through novel predictive models combined with digital biomarkers from everyday devices.

Discover the latest updates and advancements from AI-PROGNOSIS in our third newsletter edition.

Visit our website for more information: https://www.ai-prognosis.eu/.
Culicoides caldasi Browne 1980
Creators: name: Spinelli, Gustavo
name: Wolff, Marta
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Ceratopogonidae
Culicoides
Culicoides caldasi

description: caldasi Browne, 1980: 535. Type locality: Colombia, Valle del Cauca, Cali, Peñas Blancas, Finca El Rosal, 1800 m. HT F (USNM). Distr.: Colombia (Valle del Cauca (Cali, Peñas Blancas, Finca El Rosal, 1800 m)). Refs.: Browne, 1980: 535; Barreto, 1986: 145; Wirth et al., 1988: 42; Borkent & Spinelli, 2007: 72; Spinelli et al., 2009: 89; Borkent, 2015: 79 (cat.).
description: Published as part of Spinelli, Gustavo & Wolff, Marta, 2016, FAMILY CERATOPOGONIDAE, pp. 98-141 in Zootaxa 4122 (1) on page 116, DOI: 10.11646/zootaxa.4122.1.15, http://zenodo.org/record/256311
Culicoides caucaensis Wirth & Lee 1967
Creators: name: Spinelli, Gustavo
name: Wolff, Marta
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Ceratopogonidae
Culicoides
Culicoides caucaensis

description: caucaensis Wirth & Lee, 1967: 19. Type locality: Colombia, Cauca, 28.4 km E Puracé, 3100 m. HT F (# 69403 USNM). Distr.: Colombia (Cauca (28.4 km E Puracé, 3100 m)). Refs.: Wirth & Lee, 1967: 19; Barreto, 1986: 146; Wirth et al., 1988: 42; Borkent & Spinelli, 2007: 72; Spinelli et al., 2009: 89; Borkent, 2015: 80 (cat.).
description: Published as part of Spinelli, Gustavo & Wolff, Marta, 2016, FAMILY CERATOPOGONIDAE, pp. 98-141 in Zootaxa 4122 (1) on page 116, DOI: 10.11646/zootaxa.4122.1.15, http://zenodo.org/record/256311
Culicoides eublepharus Macfie 1948
Creators: name: Spinelli, Gustavo
name: Wolff, Marta
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Ceratopogonidae
Culicoides
Culicoides eublepharus

description: eublepharus Macfie, 1948: 86. Type locality: Guyana, New River. HT F (lost). Distr.: Mexico (Chiapas), Costa Rica, Colombia (Antioquia (Río Anorí), Caquetá (Tres Esquinas)) to Ecuador, Venezuela, northern Brazil. Refs.: Macfie, 1948: 86; Wirth & Blanton, 1973: 431; Barreto, 1986: 146; Wirth et al., 1988: 44; Borkent & Spinelli, 2007: 72; Spinelli et al., 2009: 89.
transferrans Ortíz, 1953: 801. Type locality: Venezuela, Amazonas, head Río Orinoco. HT F (INHC).
description: Published as part of Spinelli, Gustavo & Wolff, Marta, 2016, FAMILY CERATOPOGONIDAE, pp. 98-141 in Zootaxa 4122 (1) on page 116, DOI: 10.11646/zootaxa.4122.1.15, http://zenodo.org/record/256311
Culicoides florenciae Messersmith 1972
Creators: name: Spinelli, Gustavo
name: Wolff, Marta
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Ceratopogonidae
Culicoides
Culicoides florenciae

description: florenciae Messersmith, 1972: 167. Type locality: Colombia, Caquetá, Florencia, Ríohacha, 1000 m. HT F (# 73147 USNM). Distr.: Colombia (Caquetá (Florencia), Guajira (Ríohacha, 1000 m), Valle del Cauca (Cali, Peñas Blancas, Finca El Rosal, 1800 m)). Refs.: Messersmith, 1972: 167; Browne, 1980: 533; Barreto, 1986: 146; Wirth et al., 1988: 44; Borkent & Spinelli, 2007: 72; Spinelli et al., 2009: 89; Borkent, 2015: 84 (cat.).
description: Published as part of Spinelli, Gustavo & Wolff, Marta, 2016, FAMILY CERATOPOGONIDAE, pp. 98-141 in Zootaxa 4122 (1) on page 116, DOI: 10.11646/zootaxa.4122.1.15, http://zenodo.org/record/256311
Animalia
Creators: name: Spinelli, Gustavo
name: Wolff, Marta
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia

description: micayensis Spinelli, Santamaría, Cabrera, Ronderos & Suárez, 2009: 85. HT F (MLPA). Type locality: Colombia, Cauca, López, Río Micay. Distr.: Colombia (Cauca (López, Río Micay)). Refs.: Spinelli et al., 2009: 85; Borkent, 2015: 93 (cat.).
description: Published as part of Spinelli, Gustavo & Wolff, Marta, 2016, FAMILY CERATOPOGONIDAE, pp. 98-141 in Zootaxa 4122 (1) on page 116, DOI: 10.11646/zootaxa.4122.1.15, http://zenodo.org/record/256311
Culicoides pabloi Browne 1980
Creators: name: Spinelli, Gustavo
name: Wolff, Marta
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Ceratopogonidae
Culicoides
Culicoides pabloi

description: pabloi Browne, 1980: 541. Type locality: Colombia, Valle del Cauca, Cali, Pance, 1600 m. HT F (USNM). Distr.: Colombia (Valle del Cauca (Cali, Pance, 1600 m; Peñas Blancas)). Refs.: Browne, 1980: 541; Barreto, 1986: 147; Wirth et al., 1988: 44; Borkent & Spinelli, 2007: 72; Spinelli et al., 2009: 89; Borkent, 2015: 96 (cat.).
description: Published as part of Spinelli, Gustavo & Wolff, Marta, 2016, FAMILY CERATOPOGONIDAE, pp. 98-141 in Zootaxa 4122 (1) on page 116, DOI: 10.11646/zootaxa.4122.1.15, http://zenodo.org/record/256311
Culicoides rangeli Ortiz & Mirsa 1952
Creators: name: Spinelli, Gustavo
name: Wolff, Marta
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Ceratopogonidae
Culicoides
Culicoides rangeli

description: rangeli Ortíz & Mirsa, 1952b: 126. Type locality: Venezuela, Miranda, Los Chorros. HT F (INHC). Distr.: Mexico (Oaxaca) to Colombia. Locality in Colombia (Valle del Cauca (Cali, Peñas Blancas; Río Raposo)), Ecuador, Bolivia, Venezuela, Guadeloupe, Trinidad and Tobago, Brazil (Amazonas). Refs.: Ortíz & Mirsa, 1952b: 126; Barreto, 1986: 148; Borkent & Spinelli, 2007: 72; Spinelli et al., 2009: 89.
donajii Vargas, 1954: 28. Type locality: Mexico, Oaxaca. HT M (CAIM).
patulipalpis Wirth & Blanton, 1959: 421. Type locality: Panama, Panama prov., Cerro Campana. HT F (# 63170 USNM).
description: Published as part of Spinelli, Gustavo & Wolff, Marta, 2016, FAMILY CERATOPOGONIDAE, pp. 98-141 in Zootaxa 4122 (1) on page 116, DOI: 10.11646/zootaxa.4122.1.15, http://zenodo.org/record/256311
title: Abschlussbericht Part I: Forschungsschwerpunkt Versorgung während Schwangerschaft, Geburt und Wochenbett – Instrumente zur sektorenübergreifenden Qualitätsentwicklung – IsQua mit Graduiertenförderung an der Hochschule Osnabrück
Creators: name: zu Sayn-Wittgenstein-Hohenstein, Friederike
name: Blumenberg, Petra
name: Büscher, Andreas
name: Gorschlüter, Petra
name: Haubrock, Manfred
name: Hellmers, Claudia
name: Stelzig, Stephanie
Year: 2019
Subjects: subject: 610 Medizin, Gesundheit

description: Die Hochschule Osnabrück zeichnet sich durch einen Forschungsschwerpunkt Familienorientierte geburtshilfliche Versorgung aus, welcher auf der Forschungslandkarte der Hochschulrektorenkonferenz (HRK) verzeichnet ist. Seit 2000 werden Studien zur geburtshilflichen Versorgung durchgeführt. Im Forschungsschwerpunkt IsQua wurden in vier Teilprojekten A bis D, verschiedene Instrumente zur sektorenübergreifenden Qualitätssicherung in der Lebensphase von Schwangerschaft, Geburt und Wochenbett entwickelt, erprobt und evaluiert. Mit der Entwicklung von diesen Instrumenten wird das Ziel verfolgt, Prozesse, die die physiologische Geburt befördern, zu unterstützen. Damit soll der derzeitigen Entwicklung in der Geburtshilfe gegengesteuert werden, die u. a. von hohen Interventionsraten, wie z.B. von einer anhaltend hohen Kaiserschnittrate von 32% (IQTIG [Hg.], 2017) gekennzeichnet ist. 
Alle genannten Aspekte werden im Kontext eines Theorie Praxis Transfers gedacht. Instrumente wurden unter Beteiligung der Berufsgruppe der Hebammen heraus entwickelt. Sie wurden auf Anwenderebene – i. d. Regel durch Hebammen – und auf Ebene der Nutzerinnen bzw. Klientinnen, den schwangeren Frauen bzw. Müttern nach der Geburt, erprobt. Hierzu wurden vielfältige Kooperationen mit Einrichtungen des Gesundheitswesens geknüpft und verschiedene Zugänge eines Wissenstransfers hergestellt. Dieses war die Aufgabe von Teilprojekt E, welches horizontal zu den Teilprojekten A bis D angelegt war. Dabei wurde teilweise interdisziplinär mit Kollegen und Kolleginnen aus der Pflegewissenschaft und Gesundheitsökonomie gearbeitet. Inhaltlich stellt der Forschungsschwerpunkt ein nachgefragtes Thema zur Verbesserung der geburtshilflichen Versorgungsqualität regional und überregional dar. 
Die dem Forschungsschwerpunkt angegliederte Graduiertenförderung im Umfang von drei Stipendien eröffnete erstmalig die Möglichkeit zur strukturierten Förderung von wissenschaftlichem Nachwuchs an Fachhochschulen in der jungen Disziplin Hebammenwissenschaft. Seit 2011 kann über eine Kooperation zwischen der Hochschule Osnabrück und der privaten Universität Witten Herdecke, Fakultät Gesundheit, die Promotion verfolgt werden. Eine weitere Dissertation konnte mit Teilprojekt A assoziiert werden. Alle Promotionen waren inhaltlich mit den einzelnen Teilprojekten verbunden.
Culicoides tamboensis Wirth & Lee 1967
Creators: name: Spinelli, Gustavo
name: Wolff, Marta
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Ceratopogonidae
Culicoides
Culicoides tamboensis

description: tamboensis Wirth & Lee, 1967: 20. Type locality: Colombia, Cauca, El Tambo, Finca Carpenteria, 2500 m. HT F (# 69404 USNM). Distr.: Colombia (Cauca (El Tambo, Finca Carpenteria, 2500 m), Valle del Cauca (Cali, Pance)). Refs.: Wirth & Lee, 1967: 20; Barreto, 1986: 148; Wirth et al., 1988: 44; Borkent & Spinelli, 2007: 72; Spinelli et al., 2009: 89; Borkent, 2015: 104 (cat.).
description: Published as part of Spinelli, Gustavo & Wolff, Marta, 2016, FAMILY CERATOPOGONIDAE, pp. 98-141 in Zootaxa 4122 (1) on page 116, DOI: 10.11646/zootaxa.4122.1.15, http://zenodo.org/record/256311
title: Maßnahmen zur Innovation in der Lehre im digitalen Zeitalter: Rahmenbedingungen und Anreizsysteme für Lehrende
Creators: name: Stelzig, Stephanie
Publisher: name: Hochschule Osnabrück
Year: 2020
Subjects: subject: 650 Management

description: Vor dem Hintergrund der Auseinandersetzung mit der Digitalisierung im Hochschulsystem werden ausgewählte Aktivitäten für eine innovative Lehre in den Fokus genommen. Im Mittelpunkt der Betrachtung stehen die Hochschullehrenden. Zentrale Fragen, denen dabei nachgegangen wird, lauten: Wie können Lehrende im digitalen Zeitalter ihre Lehre innovativ gestalten? Welche Rahmenbedingungen und Anreizsysteme in der Lehr- Lernkultur von Hochschulen für angewandte Wissenschaften sind dafür notwendig, um eine in die Breite zielende Teilhabe an digitalen Lehr-Lernformaten zu erreichen? In einem exemplarischen Überblick bereits realisierter Vorhaben aus dem nationalen Hochschulraum werden Good-Practice-Beispiele, deren Wirksamkeit teilweise dokumentiert ist, skizziert. Die aus dieser Übersicht abgeleiteten Maßnahmen können im weiteren Prozess der Erschließung digitaler Chancen für die Lehre an der Hochschule Osnabrück zur Orientierung dienen.
Culicoides balsapambensis Ortiz & Leon 1955
Creators: name: Spinelli, Gustavo
name: Wolff, Marta
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Ceratopogonidae
Culicoides
Culicoides balsapambensis

description: balsapambensis Ortíz & León, 1955: 569 (as variety of pifanoi). Type locality: Ecuador, Bolivar, Balsapamba. ST F (UNCE). Distr.: Costa Rica to Colombia (Valle del Cauca (Cali, Pance)), Ecuador, Brazil. Refs.: Ortíz & León, 1955: 569; Barreto, 1986: 145; Borkent & Spinelli, 2007: 72; Spinelli et al., 2009: 89.
description: Published as part of Spinelli, Gustavo & Wolff, Marta, 2016, FAMILY CERATOPOGONIDAE, pp. 98-141 in Zootaxa 4122 (1) on page 116, DOI: 10.11646/zootaxa.4122.1.15, http://zenodo.org/record/256311
Culicoides castillae Fox 1946
Creators: name: Spinelli, Gustavo
name: Wolff, Marta
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Ceratopogonidae
Culicoides
Culicoides castillae

description: castillae Fox, 1946: 251. Type locality: Honduras, Puerta Castilla. HT F (UNPR). Distr.: Guatemala to Colombia (Boyacá (Pauna, Topo Grande), Caquetá (Solano)), Ecuador, Venezuela, Trinidad and Tobago. Refs.: Fox, 1946: 251; Borkent & Spinelli, 2007: 72; Spinelli et al., 2009: 86.
gibsoni Wirth, 1952: 246. Type locality: Guatemala, Chimaltenango, San Pedro Yepocapa. HT F (# 61407 USNM). flochabonnenci Ortíz & Mirsa, 1952a: 267. Type locality: Venezuela, Miranda, Los Chorros. HT F (INHC).
description: Published as part of Spinelli, Gustavo & Wolff, Marta, 2016, FAMILY CERATOPOGONIDAE, pp. 98-141 in Zootaxa 4122 (1) on page 116, DOI: 10.11646/zootaxa.4122.1.15, http://zenodo.org/record/256311
title: Les matrices disciplinaires du design
Publisher: name: Université Toulouse - Jean Jaurès
Year: 2024

description: Le terme de design est aujourd’hui revendiqué dans de nombreuses pratiques (design thinking, design management, design strategy, design innovation), au point que l’on peut se demander ce qui constitue encore l’unité de la discipline. Dans une telle situation, réfléchir aux matrices du design, aux contextes et aux enjeux qui lui ont permis de prendre forme, pourrait permettre de repenser à nouveaux frais ce dont le design peut être porteur.
Culicoides fluvialis Macfie 1940
Creators: name: Spinelli, Gustavo
name: Wolff, Marta
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Ceratopogonidae
Culicoides
Culicoides fluvialis

description: fluvialis Macfie, 1940c: 25. Type locality: Guyana, New River. HT F (BMNH). Distr.: Honduras to Colombia (?), Venezuela, Trinidad and Tobago, Guyana, Brazil (Amazonas, Pará). Refs.: Macfie, 1940c: 25; Wirth et al., 1988: 44; Borkent & Spinelli, 2007: 72; Spinelli et al., 2009: 86.
description: Published as part of Spinelli, Gustavo & Wolff, Marta, 2016, FAMILY CERATOPOGONIDAE, pp. 98-141 in Zootaxa 4122 (1) on page 117, DOI: 10.11646/zootaxa.4122.1.15, http://zenodo.org/record/256311
Culicoides leopoldoi Ortiz 1951
Creators: name: Spinelli, Gustavo
name: Wolff, Marta
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Ceratopogonidae
Culicoides
Culicoides leopoldoi

description: leopoldoi Ortíz, 1951: 579. Type locality: Venezuela, Miranda, Ocumare del Tuy. HT F (INHC). Distr.: Guatemala and Belize to Bolivia and northeastern Argentina, Trinidad and Tobago, Colombia (Valle del Cauca (Río Raposo)). Refs.: Ortíz, 1951: 579; Barreto, 1986: 147; Borkent & Spinelli, 2007: 72; Spinelli et al., 2009: 90.
description: Published as part of Spinelli, Gustavo & Wolff, Marta, 2016, FAMILY CERATOPOGONIDAE, pp. 98-141 in Zootaxa 4122 (1) on page 117, DOI: 10.11646/zootaxa.4122.1.15, http://zenodo.org/record/256311
Culicoides fieldi Wirth & Blanton 1956
Creators: name: Spinelli, Gustavo
name: Wolff, Marta
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Ceratopogonidae
Culicoides
Culicoides fieldi

description: fieldi Wirth & Blanton, 1956c: 50. Type locality: Honduras, Lancetilla. HT F (# 63130 USNM). Distr.: Honduras, Costa Rica, Panama, Colombia (Valle del Cauca (Río Raposo)). Refs.: Wirth & Blanton, 1956c: 50; Barreto, 1986: 146; Borkent & Spinelli, 2007: 73; Spinelli et al., 2009: 90.
description: Published as part of Spinelli, Gustavo & Wolff, Marta, 2016, FAMILY CERATOPOGONIDAE, pp. 98-141 in Zootaxa 4122 (1) on page 117, DOI: 10.11646/zootaxa.4122.1.15, http://zenodo.org/record/256311
title: Skulpturen
title: Plastiken - Skulpturen - Skulptouren in Horgen
titleType: Subtitle
Creators: name: Caflisch, Albert
name: Erdin, Hans
Publisher: name: Gemeinde Horgen
Year: 2003
Subjects: subject: 730 Plastische Künste, Bildhauerkunst
subject: Horgen
subject: Heimatkunde
subject: Kunst
subject: Plastik
subject: Skulpturenpark

description: Enthält eine Chronik der Gemeinde Horgen für 2002
Culicoides gabaldoni Ortiz 1954
Creators: name: Spinelli, Gustavo
name: Wolff, Marta
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Ceratopogonidae
Culicoides
Culicoides gabaldoni

description: gabaldoni Ortíz, 1954: 221. Type locality: Venezuela, Yaracuy, San Felipe. HT F (INHC). Distr.: Mexico (Tabasco) to Colombia (Valle del Cauca (Río Raposo)), Ecuador, Venezuela, Trinidad and Tobago, Brazil (Bahia), Paraguay, northeastern Argentina. Refs.: Ortíz, 1954: 221; Barreto, 1986: 146; Borkent & Spinelli, 2007: 73; Spinelli et al., 2009: 90.
description: Published as part of Spinelli, Gustavo & Wolff, Marta, 2016, FAMILY CERATOPOGONIDAE, pp. 98-141 in Zootaxa 4122 (1) on page 117, DOI: 10.11646/zootaxa.4122.1.15, http://zenodo.org/record/256311
Culicoides glabellus Wirth & Blanton 1956
Creators: name: Spinelli, Gustavo
name: Wolff, Marta
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Ceratopogonidae
Culicoides
Culicoides glabellus

description: glabellus Wirth & Blanton, 1956c: 47. Type locality: Panama, Bocas del Toro, Almirante. HT F (# 63129 USNM). Distr.: Honduras to Colombia (Putumayo (Puerto Leguizamo), Valle del Cauca (Río Raposo)), Ecuador, Trinidad and Tobago, Brazil (Bahia, Pará). Refs.: Wirth & Blanton, 1956c: 47; Wirth & Blanton, 1973: 437; Barreto, 1986: 147; Borkent & Spinelli, 2007: 73; Spinelli et al., 2009: 90.
description: Published as part of Spinelli, Gustavo & Wolff, Marta, 2016, FAMILY CERATOPOGONIDAE, pp. 98-141 in Zootaxa 4122 (1) on page 117, DOI: 10.11646/zootaxa.4122.1.15, http://zenodo.org/record/256311
title: Measuring deep poverty in developed countries. A cumulative indicator of income and material deprivation  (LIEPP Working Paper, n°155)
Creators: name: Blasco, Julien
Publisher: name: Sciences Po - LIEPP
Year: 2023
Subjects: classificationCode: 5
subject: FOS: Social sciences

description: Do workers speak their mind about sexism and about diversity, equity, and inclusion (DEI) policies in the workplace? We measure social desirability bias regarding sexism and DEI policies using a list experiment survey among workers from five male-dominated industries in France and in the US. In both countries and, remarkably, among both men and women, we document substantial social desirability bias. Managers exhibit a larger bias than non-managerial employees. This difference between voiced and real attitudes may make organizations overestimate support for DEI policies in their workforce, rendering such policies less effective.
Culicoides leoni Barbosa 1952
Creators: name: Spinelli, Gustavo
name: Wolff, Marta
Publisher: name: Zenodo
Year: 2016
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Ceratopogonidae
Culicoides
Culicoides leoni

description: leoni Barbosa, 1952: 17 (1953: 19). Type locality: Ecuador, Santo Domingo. HT F (UNCE). Distr.: Colombia (Boyacá (Pauna, Topo Grande; Aguasal)), Ecuador. Refs.: Barbosa, 1952, 17 (1953: 19); Borkent & Spinelli, 2007: 73; Spinelli et al., 2009: 87.
description: Published as part of Spinelli, Gustavo & Wolff, Marta, 2016, FAMILY CERATOPOGONIDAE, pp. 98-141 in Zootaxa 4122 (1) on page 117, DOI: 10.11646/zootaxa.4122.1.15, http://zenodo.org/record/256311
Model-Guided Design and Optimization of CPA Perfusion Protocols for Whole Organ Cryopreservation
Creators: name: Han, Zonghu
name: Rao, Joseph Sushil
name: Ramesh, Srivasupradha
name: Hergesell, Jan
name: Namsrai, Bat-Erdene
name: Etheridge, Michael L.
name: Finger, Erik B.
name: Bischof, John C.
Publisher: name: Dordrecht [u.a.] : Springer Science + Business Media B.V
Year: 2023
Subjects: subject: Cryoprotectant
subject: Organ perfusion optimization
subject: Organ vitrification
subject: Toxicity
subject: Transport
subject: Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit

description: Vitrification could enable long-term organ preservation, but only after loading high-concentration, potentially toxic cryoprotective agents (CPAs) by perfusion. In this paper, we combine a two-compartment Krogh cylinder model with a toxicity cost function to theoretically optimize the loading of CPA (VMP) in rat kidneys as a model system. First, based on kidney perfusion experiments, we systematically derived the parameters for a CPA transport loading model, including the following: V b = 86.0% (r a = 3.86 μm), L p = 1.5 × 10–14 m3/(N·s), ω = 7.0 × 10–13 mol/(N·s), σ = 0.10. Next, we measured the toxicity cost function model parameters as α = 3.12 and β = 9.39 × 10–6. Combining these models, we developed an improved kidney-loading protocol predicted to achieve vitrification while minimizing toxicity. The optimized protocol resulted in shorter exposure (25 min or 18.5% less) than the gold standard kidney-loading protocol for VMP, which had been developed based on decades of empirical practice. After testing both protocols on rat kidneys, we found comparable physical and biological outcomes. While we did not dramatically reduce toxicity, we did reduce the time. As our approach is now validated, it can be used on other organs lacking defined toxicity data to reduce CPA exposure time and provide a rapid path toward developing CPA perfusion protocols for other organs and CPAs.
Library of prefabricated locked nucleic acid hydrolysis probes facilitates rapid development of reverse-transcription quantitative real-time PCR assays for detection of novel influenza A/H1N1/09 virus.
Creators: name: Wenzel, Jürgen J.
name: Walch, Heiko
name: Bollwein, Markus
name: Niller, Hans Helmut
name: Ankenbauer, Waltraud
name: Mauritz, Ralf
name: Höltke, Hans-Joachim
name: Zepeda, Héctor Manuel
name: Wolf, Hans
name: Jilg, Wolfgang
name: Reischl, Udo
Publisher: name: Universität Regensburg
Year: 2009
Subjects: subject: Influenza A/H1N1/09; Locked Nucleic Acid Hydrolysis Probes; Molecular Detection ; Real-Time RT-PCR; Universal ProbeLibrary
subject: 570 Biowissenschaften, Biologie
subject: 500 Naturwissenschaften
subject: 610 Medizin

description: BACKGROUND: The emergence of a novel pandemic human strain of influenza A (H1N1/09) has clearly demonstrated the need for flexible tools enabling the rapid development of new diagnostic methods. METHODS: We designed a set of reverse-transcription quantitative real-time PCR (RT-qPCR) assays based on the Universal ProbeLibrary (UPL)--a collection of 165 presynthesized, fluorescence-labeled locked nucleic acid (LNA) hydrolysis probes--specifically to detect the novel influenza A virus. We evaluated candidate primer/UPL-probe pairs with 28 novel influenza A/H1N1/09 patient samples of European and Mexican origin. RESULTS: Of 14 assays in the hemagglutinin (HA) and neuraminidase (NA) genes, 12 detected viral nucleic acids from diluted patient samples without need for further optimization. We characterized the diagnostic specificity of the 2 best-performing assays with a set of samples comprising various influenza virus strains of human and animal origin that showed no cross-reactivity. The diagnostic sensitivity of these 2 primer/probe combinations was in the range of 100-1000 genomic copies/mL. In comparison to a reference assay recommended by the German health authorities, the analytical sensitivities and specificities of the assays were equivalent. CONCLUSIONS: Facing the emergence of novel influenza A/H1N1/09, we were able to develop, within 2 days, a set of sensitive and specific RT-qPCR assays for the laboratory diagnosis of suspected cases. H1N1/09 served as a model to show the feasibility of the UPL approach for the expedited development of new diagnostic assays to detect emerging pathogens.