cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Isotopologue Profiling of Legionella pneumophila
Creators: name: Eylert, Eva
name: Herrmann, Vroni
name: Jules, Matthieu
name: Gillmaier, Nadine
name: Lautner, Monika
name: Buchrieser, Carmen
name: Eisenreich, Wolfgang
name: Heuner, Klaus
Publisher: name: Robert Koch-Institut
Year: 2010
Subjects: subject: Legionella pneumophila/genetics
subject: Legionella pneumophila/growth & development
subject: Legionella pneumophila/metabolism
subject: Bacterial Proteins/metabolism
subject: Models Biological
subject: Acanthamoeba castellanii/microbiology
subject: Magnetic Resonance Spectroscopy
subject: Carbohydrate Metabolism
subject: Carbon/metabolism
subject: Carbon Isotopes
subject: Culture Media
subject: Gas Chromatography-Mass Spectrometry
subject: Glucose/metabolism
subject: Hydroxybutyrates/chemistry
subject: Hydroxybutyrates/metabolism
subject: Metabolic Networks and Pathways
subject: Metabolomics/methods
subject: Methylene Chloride
subject: Mutation/genetics
subject: Polyesters/chemistry
subject: Polyesters/metabolism
subject: Serine/metabolism
subject: 610 Medizin

description: Legionella pneumophila (Lp) is commonly found in freshwater habitats but is also the causative agent of Legionnaires' disease when infecting humans. Although various virulence factors have been reported, little is known about the nutrition and the metabolism of the bacterium. Here, we report the application of isotopologue profiling for analyzing the metabolism of L. pneumophila. Cultures of Lp were supplied with [U-(13)C(3)]serine, [U-(13)C(6)]glucose, or [1,2-(13)C(2)]glucose. After growth, (13)C enrichments and isotopologue patterns of protein-derived amino acids and poly-3-hydroxybutyrate were determined by mass spectrometry and/or NMR spectroscopy. The labeling patterns detected in the experiment with [U-(13)C(3)]serine showed major carbon flux from serine to pyruvate and from pyruvate to acetyl-CoA, which serves as a precursor of poly-3-hydroxybutyrate or as a substrate of a complete citrate cycle with Si specificity of the citrate synthase. Minor carbon flux was observed between pyruvate and oxaloacetate/malate by carboxylation and decarboxylation, respectively. The apparent lack of label in Val, Ile, Leu, Pro, Phe, Met, Arg, and Tyr confirmed that L. pneumophila is auxotrophic for these amino acids. Experiments with [(13)C]glucose showed that the carbohydrate is also used as a substrate to feed the central metabolism. The specific labeling patterns due to [1,2-(13)C(2)]glucose identified the Entner-Doudoroff pathway as the predominant route for glucose utilization. In line with these observations, a mutant lacking glucose-6-phosphate dehydrogenase (Delta zwf) did not incorporate label from glucose at significant levels and was slowly outcompeted by the wild type strain in successive rounds of infection in Acanthamoeba castellanii, indicating the importance of this enzyme and of carbohydrate usage in general for the life cycle of Lp.
POPULATION GENETICS OF CREEK CHUB (SEMOTILUS ATROMACULATUS) IN A POSTGLACIAL, AGRICULTURAL LANDSCAPE
Creators: name: Schnelker, Abigail Ranee
Publisher: name: Purdue University Graduate School
Year: 2019
Subjects: Ecology not elsewhere classified

description: The population genetics of species occupying formerly glaciated regions are not only impacted by glacial retreat but also agricultural land use that is typical of such regions. Areas which have experienced glaciation often display a lowered amount of genetic variability and minimal population structure, and these effects become more predominant with increasing distance from a potential refugial population. Meanwhile, agricultural land use over the recent past has also been demonstrated to disrupt population structure distribution through disturbance regimes. The purpose of this study was to assess potential post-glacial and agricultural effects on populations of creek chub (Semotilus atromaculatus) in two agricultural watersheds that differ in the glacial history. The Saint Joseph River (SJR) watershed, Indiana and Michigan, USA was entirely glaciated during the last glacial maxima, while the Little Miami River (LMR) watershed in Ohio, USA, is situated on the boundary of the glacier. The degree of agricultural land use also varies between and within the two watersheds. Using eight microsatellite loci, 312 individuals were genotyped from 13 sites in SJR and 2,318 individuals from 29 sites in LMR. Measures of genetic differentiation showed that there was strong differentiation between watersheds. Analyses within watersheds recovered additional but weaker differentiation that was mostly associated with the geography of sub-watersheds and isolation by distance. Proximity to the glacial boundary appeared to play a minimal role in genetic differentiation and genetic variation. Differentiation among localities was not directly associated with the glacial boundary within LMR, and localities in this watershed had lower allelic richness and heterozygosity than those in the fully glaciated SJR. After accounting for the positive correlation of stream distance in LMR using partial Mantel test, both glacial history and agricultural land use were positively correlated with genetic differentiation. However, these predictor variables were also strongly correlated with one another which prevented disentangling the two potential effects. Within SJR, no 10 relationship of genetic differentiation with agricultural land use was recovered. My study shows that there is not a simple relationship between glacial history, contemporary land use, and genetic differentiation in creek chub. Rather, it appears that the patterns of genetic variation observed may be more closely linked to the dispersal behavior of creek chub within and among watersheds, and the history of effective population size within watersheds.
Serum-free freezing media support high cell quality and excellent ELISPOT assay performance across a wide variety of different assay protocols
Creators: name: Filbert, Helene
name: Attig, Sebastian
name: Bidmon, Nicole
name: Renard, Bernhard Y.
name: Janetzki, Sylvia
name: Sahin, Ugur
name: Welters, Marij J. P.
name: Ottensmeier, Christian
name: Burg, Sjoerd H. Van Der
name: Gouttefangeas, Cécile
name: Britten, Cedrik M.
Publisher: name: Robert Koch-Institut
Year: 2012
Subjects: subject: Animals
subject: Humans
subject: Cattle
subject: Cell Survival
subject: Culture Media
subject: Serum-Free/chemistry
subject: Enzyme-Linked Immunospot Assay/methods
subject: Enzyme-Linked Immunospot Assay/standards
subject: Epitopes T-Lymphocyte/analysis
subject: Epitopes T-Lymphocyte/immunology
subject: Freezing
subject: HLA-A2 Antigen/chemistry
subject: HLA-A2 Antigen/immunology
subject: Interferon-gamma/chemistry
subject: Interferon-gamma/immunology
subject: Leukocytes Mononuclear/chemistry
subject: Leukocytes Mononuclear/immunology
subject: Peptide Fragments/chemistry
subject: Peptide Fragments/immunology
subject: T-Lymphocytes/chemistry
subject: T-Lymphocytes/immunology
subject: 610 Medizin

description: Robust and sensitive ELISPOT protocols are commonly applied concomitant with the development of new immunotherapeutics. Despite the knowledge that individual serum batches differ in their composition and may change properties over time, serum is still commonly used in immunologic assays. Commercially available serum batches are expensive, limited in quantity and need to be pretested for suitability in immunologic assays, which is a laborious process. The aim of this study was to test whether serum-free freezing media can lead to high cell viability and favorable performance across multiple ELISPOT assay protocols. Thirty-one laboratories from ten countries participated in a proficiency panel organized by the Cancer Immunotherapy Immunoguiding Program to test the influence of different freezing media on cell quality and immunologic function. Each center received peripheral blood mononuclear cells which were frozen in three different media. The participants were asked to quantify antigen-specific CD8+ T-cell responses against model antigens using their locally established IFN-gamma ELISPOT protocols. Self-made and commercially available serum-free freezing media led to higher cell viability and similar cell recovery after thawing and resting compared to freezing media supplemented with human serum. Furthermore, the test performance as determined by (1) background spot production, (2) replicate variation, (3) frequency of detected antigen-specific spots and (4) response detection rate was similar for serum and serum-free conditions. We conclude that defined and accessible serum-free freezing media should be recommended for freezing cells stored for subsequent ELISPOT analysis.
A PASSIVE SAFETY APPROACH TO EVALUATE SPACECRAFT RENDEZVOUS MISSION RISK
Creators: name: Goggin, McClain M
Publisher: name: Purdue University Graduate School
Year: 2019
Subjects: Aerospace engineering not elsewhere classified

description: Orbital rendezvous enables spacecraft to perform missions to service satellites, remove space debris, resupply space stations, and return samples from other planets. These missions are often considered high risk due to concerns that the two spacecraft will collide if the maneuvering capability of one spacecraft is compromised by a fault.
In this thesis, a passive safety analysis is used to evaluate the probability that a fault that compromises maneuvering capability results in a collision. For a rendezvous
mission, the chosen approach trajectory, state estimation technique, and probability of collision calculation each impact the total collision probability of the mission. This
thesis presents a modular framework for evaluating the comparing the probability of collision of rendezvous mission design concepts.
Trade studies were performed using a baseline set of approach trajectories, and a Kalman Filter for relative state estimation and state estimate uncertainty. The state covariance matrix following each state update was used to predict the resulting probability of collision if a fault were to occur at that time. These trade studies emphasize that the biggest indicator of rendezvous mission risk is the time spent on a nominal intercept trajectory.
sj-pdf-5-jrt-10.1177_20556683211012885 - Supplemental material for Novel integrative rehabilitation system for the upper extremity: Design and usability evaluation
Creators: name: Burdea, Grigore
name: Kim, Nam
name: Polistico, Kevin
name: Kadaru, Ashwin
name: Roll, Doru
name: Grampurohit, Namrata
Publisher: name: SAGE Journals
Year: 2021
Subjects: subject: 99999 Engineering not elsewhere classified
subject: FOS: Other engineering and technologies

description: Supplemental material, sj-pdf-5-jrt-10.1177_20556683211012885 for Novel integrative rehabilitation system for the upper extremity: Design and usability evaluation by Grigore Burdea, Nam Kim, Kevin Polistico, Ashwin Kadaru, Doru Roll and Namrata Grampurohit in Journal of Rehabilitation and Assistive Technologies Engineering
sj-pdf-4-bbi-10.1177_11779322211030364 – Supplemental material for DEELIG: A Deep Learning Approach to Predict Protein-Ligand Binding Affinity
Creators: name: Ahmed, Asad
name: Mam, Bhavika
name: Sowdhamini, Ramanathan
Publisher: name: SAGE Journals
Year: 2021
Subjects: subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences

description: Supplemental material, sj-pdf-4-bbi-10.1177_11779322211030364 for DEELIG: A Deep Learning Approach to Predict Protein-Ligand Binding Affinity by Asad Ahmed, Bhavika Mam and Ramanathan Sowdhamini in Bioinformatics and Biology Insights
Revolution, Trauer, Tragik. Der zweite Teil der "Dziady" von Adam Mickiewicz als Trauerspiel
Creators: name: Junkiert, Maciej
Publisher: name: Peter Lang International
Year: 2017
Subjects: subject: Revolution (France : 1789-1799)
subject: Intellectual life
subject: Polish literature
subject: History
subject: Romanticism

description: Der Vergleich der Auswirkungen der Französischen Revolution auf die Literatur und Kultur in Polen und in den deutschen Staaten ist sehr schwierig. Die polnischen Schriftsteller und Denker der ersten und zweiten Romantikergeneration sind zu spät geboren worden, um Zeugen der Französichen Revolution zu sein. Für die Älteren, wie Kazimierz Brodziński, Aleksander Fredro und Antoni Malczewski werden die Napoleonischen Kriege, insbesondere die Feldzüge von 1807, 1809 und 1812, zur Generationserfahrung. Für die Jüngeren, wie Adam Mickiewicz, Juliusz Słowacki und Zygmunt Krasiński wird das erst der Novemberaufstand sein. Aber es gibt etwas, was sie zusammen als eine für die polnische Kultur besondere Generationenformation erscheinen lässt: Es ist ein für die Polen bahnbrechendes Projekt, das gerade in der Romantik ins Leben gerufen wurde, ein Projekt der Neugestaltung der polnischen Identität in Anlehnung an Kultur, Literatur und die slavische Spiritualität, gefiltert durch das Christentum. Schlüsselmomente dieser Einstellung waren die Erkenntnis der tragischen Situation, in der sich die ganze Nation befand, und die Umorientierung der Aufmerksamkeit auf die Zukunft, von der man eine Chance auf die Wiederherstellung des Vaterlandes erwartete. Dieser Blick nach vorn und die Konzeption der Neugestaltung des Volkes im Geiste der Kulturnation bilden eine besondere polnisch-deutsche Vergleichsebene für die Situation in den ersten Jahrzehnten des 19. Jahrhunderts.
iPiG: Integrating Peptide Spectrum Matches into Genome Browser Visualizations
Creators: name: Kuhring, Mathias
name: Renard, Bernhard Y.
Publisher: name: Robert Koch-Institut
Year: 2012
Subjects: subject: Computer Graphics
subject: Databases Protein
subject: Genomics
subject: Peptides/chemistry
subject: Peptides/genetics
subject: Proteomics
subject: User-Computer Interface
subject: 610 Medizin

description: Proteogenomic approaches have gained increasing popularity, however it is still difficult to integrate mass spectrometry identifications with genomic data due to differing data formats. To address this difficulty, we introduce iPiG as a tool for the integration of peptide identifications from mass spectrometry experiments into existing genome browser visualizations. Thereby, the concurrent analysis of proteomic and genomic data is simplified and proteomic results can directly be compared to genomic data. iPiG is freely available from https://sourceforge.net/projects/ipig/. It is implemented in Java and can be run as a stand-alone tool with a graphical user-interface or integrated into existing workflows. Supplementary data are available at PLOS ONE online.
Prevalence of HIV among MSM in Europe: comparison of self-reported diagnoses from a large scale internet survey and existing national estimates
Creators: name: Marcus, Ulrich
name: Hickson, Ford
name: Weatherburn, Peter
name: Schmidt, Axel J.
Publisher: name: Robert Koch-Institut, Infektionsepidemiologie
Year: 2012
Subjects: subject: HIV prevalence
subject: Men having sex with men (MSM)
subject: Seroprevalence studies
subject: Internet survey
subject: 610 Medizin

description: Background: Country level comparisons of HIV prevalence among men having sex with men (MSM) is challenging for a variety of reasons, including differences in the definition and measurement of the denominator group, recruitment strategies and the HIV detection methods. To assess their comparability, self-reported data on HIV diagnoses in a 2010 pan-European MSM internet survey (EMIS) were compared with pre-existing estimates of HIV prevalence in MSM from a variety of European countries. Methods: The first pan-European survey of MSM recruited more than 180,000 men from 38 countries across Europe and included questions on the year and result of last HIV test. HIV prevalence as measured in EMIS was compared with national estimates of HIV prevalence based on studies using biological measurements or modelling approaches to explore the degree of agreement between different methods. Existing estimates were taken from Dublin Declaration Monitoring Reports or UNAIDS country fact sheets, and were verified by contacting the nominated contact points for HIV surveillance in EU/EEA countries. Results: The EMIS self-reported measurements of HIV prevalence were strongly correlated with existing estimates based on biological measurement and modelling studies using surveillance data (R2=0.70 resp. 0.72). In most countries HIV positive MSM appeared disproportionately likely to participate in EMIS, and prevalences as measured in EMIS are approximately twice the estimates based on existing estimates. Conclusions: Comparison of diagnosed HIV prevalence as measured in EMIS with pre-existing estimates based on biological measurements using varied sampling frames (e.g. Respondent Driven Sampling, Time and Location Sampling) demonstrates a high correlation and suggests similar selection biases from both types of studies. For comparison with modelled estimates the self-selection bias of the Internet survey with increased participation of men diagnosed with HIV has to be taken into account. For most countries self-reported EMIS prevalence is higher than measured prevalence, which is likely due to a combination of different time points of measurement, measurement errors for small sample sizes, different sampling methods, and an indicator-inherent overestimate of prevalence among the untested fraction of MSM.
School Active Shooter Prevention Measurements
Creators: name: Reichart, Katherine E
Publisher: name: Purdue University Graduate School
Year: 2019
Subjects: Causes and prevention of crime

description: This research investigated how to measure school active shooter safety against current policies in place regarding two different areas of school climate. Using the State of Indiana as a case study, 55 different schools from 38 different counties, various socioeconomic environments and school types (public, private, etc.) were surveyed. This collected data was used to represent a sample and representation of active shooter safety in K- 12 schools in Indiana. Research was conducted through a survey of approximately 40 questions posed to the principal of a school. The survey was anonymously distributed, and any identifiable information was assigned a numerical code. Anonymized demographics were considered and measured as well to determine how active shooter prevention is treated amongst them. After the study was conducted, the data shows how demographics, policies, and procedures affect school active shooter prevention. Analysis showed that school size may relate to lower social emotional security scores. Additionally, middle schools appear to score higher on social-emotional security than K-12 schools. Nonpublic schools also appear to score less on active shooter prevention than public schools. There is a moderate effect between the two. Schools and policymakers should account for this when developing active shooter safety plans.
Additional file 1 of Online peer-led intervention to improve adolescent wellbeing during the COVID-19 pandemic: a randomised controlled trial
Creators: name: Pavarini, Gabriela
name: Reardon, Tessa
name: Mawdsley, Geoffrey
name: Singh, Ilina
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Neuroscience
subject: Physiology
subject: FOS: Biological sciences
subject: Pharmacology
subject: Biotechnology
subject: Sociology
subject: FOS: Sociology
subject: Cancer
subject: Mental Health
subject: Plant Biology

description: Additional file 1. Description of data Supplementary tables containing details of YPAG contributions and results.
Contact investigation of a case of human novel coronavirus infection treated in a German hospital, October-November 2012
Creators: name: Buchholz, Udo
name: Müller, Marcel A.
name: Nitsche, Andreas
name: Sanewski, A.
name: Wevering, N.
name: Bauer-Balci, T.
name: Bonin, F.
name: Drosten, Christian
name: Schweiger, Brunhilde
name: Wolff, Thorsten
name: Muth, D.
name: Meyer, B.
name: Buda, Silke
name: Krause, Gérard
name: Schaade, Lars
name: Haas, Walter
Publisher: name: Robert Koch-Institut, Infektionsepidemiologie; Robert Koch-Institut, Biologische Sicherheit
Year: 2013
Subjects: subject: 610 Medizin

description: On 24 October 2012, a patient with acute respiratory distress syndrome of unknown origin and symptom onset on 5 October was transferred from Qatar to a specialist lung clinic in Germany. Late diagnosis on 20 November of an infection with the novel Coronavirus (NCoV) resulted in potential exposure of a considerable number of healthcare workers. Using a questionnaire we asked 123 identified contacts (120 hospital and three out-of-hospital contacts) about exposure to the patient. Eighty-five contacts provided blood for a serological test using a two-stage approach with an initial immunofluorescence assay as screening test, followed by recombinant immunofluorescence assays and a NCoV-specific serum neutralisation test. Of 123 identified contacts nine had performed aerosol-generating procedures within the third or fourth week of illness, using personal protective equipment rarely or never, and two of these developed acute respiratory illness. Serology was negative for all nine. Further 76 hospital contacts also tested negative, including two sera initially reactive in the screening test. The contact investigation ruled out transmission to contacts after illness day 20. Our two-stage approach for serological testing may be used as a template for similar situations.
sj-docx-1-cad-10.1177_00111287211029873 – Supplemental material for Understanding Knife Crime and Trust in Police with Young People in East London
Creators: name: Skarlatidou, Artemis
name: Ludwig, Lina
name: Solymosi, Reka
name: Bradford, Ben
Publisher: name: SAGE Journals
Year: 2021
Subjects: subject: 160299 Criminology not elsewhere classified
subject: FOS: Law

description: Supplemental material, sj-docx-1-cad-10.1177_00111287211029873 for Understanding Knife Crime and Trust in Police with Young People in East London by Artemis Skarlatidou, Lina Ludwig, Reka Solymosi and Ben Bradford in Crime & Delinquency
Size Dependent Failure Constrained Topology Optimization Approaches
Creators: name: Vernacchio, Vincenzo G
Publisher: name: Purdue University Graduate School
Year: 2019
Subjects: Mechanical engineering not elsewhere classified

description: New approaches in topology optimization and manufacturing techniques are generating multi-scale, physically realized mechanical components from advanced materials. Current optimization formulations do not consider the dependence of strength on feature size. By failing to account for the mechanical models of this behavior, sub-optimal structures are generated.
A currently available academic density-based topology optimization code is extended to incorporate strength constraints. A continuum theory of failure novel to the optimization field is implemented to account for both general yielding and fracture dominated failure. The fracture limit is then formulated in terms of well-established models of brittle and quasi-brittle size dependence. Additional models of size dependence based on assumed flaw sizes are considered using the theory of linear elastic fracture mechanics. To unify the optimized topology and the empirical geometric-scaling models used, a novel geometric measure of local size is proposed. This measure interprets the evolving density field using a consistent domain of support and maintains consistency with gradient-based methods of optimization. The geometric measure is evaluated using test-problems which consider a minimum compliance objective under geometric constraints.
The resulting optimized structures are presented for the geometric and size-dependent strength constrained formulations. The geometrically constrained results illustrate the flexibility and robustness of the proposed local size measure. The various models of size-dependent strength illustrate the impact and necessity of considering physical models of material within the topology optimization formulation. Results which exhibit clear "micro-structural" features and scale transitioning architectures are presented for limited multi-scale optimization studies.
An attempt at physical validation considering a single model of quasi-brittle material failure is made. Existing approaches for generating 3D volumetric meshes from image data are leveraged to yield CAD interpretations of optimized structures. Structures are then printed using a 3D printing PolyJet process with a previously established size-dependent material. Structures are destructively evaluated under displacement controlled load testing. The resulting tests indicate that the stress states in the structure fail to induce the expected size-dependent material characteristics. Furthermore, the testing results indicate the difficulty in properly accounting for boundary conditions in the topology optimization approach.
Highly Pathogenic H5N1 Influenza A Virus Strains Provoke Heterogeneous IFN-α/β Responses That Distinctively Affect Viral Propagation in Human Cells
Creators: name: Matthaei, Markus
name: Budt, Matthias
name: Wolff, Thorsten
Publisher: name: Robert Koch-Institut, Infektionskrankheiten / Erreger
Year: 2013
Subjects: subject: Animals
subject: Humans
subject: Enzyme-Linked Immunosorbent Assay
subject: Interferon-beta/metabolism
subject: Birds
subject: Cell Line
subject: Immunoblotting
subject: Influenza A Virus H5N1 Subtype/immunology
subject: Influenza A Virus H5N1 Subtype/pathogenicity
subject: Interferon-alpha/metabolism
subject: Virus Replication/immunology
subject: 610 Medizin

description: The fatal transmissions of highly pathogenic avian influenza A viruses (IAV) of the H5N1 subtype to humans and high titer replication in the respiratory tract indicate that these pathogens can overcome the bird-to-human species barrier. While type I interferons (IFN-α/β) are well described to contribute to the species barrier of many zoonotic viruses, current data to the role of these antiviral cytokines during human H5N1 IAV infections is limited and contradictory. We hypothesized an important role for the IFN system in limiting productive infection of avian H5N1 strains in human cells. Hence, we examined IFN-α/β gene activation by different avian and human H5N1 isolates, if the IFN-α/β response restricts H5N1 growth and whether the different strains were equally capable to regulate the IFN-α/β system via their IFN-antagonistic NS1 proteins. Two human H5N1 isolates and a seasonal H3N2 strain propagated efficiently in human respiratory cells and induced little IFN-β, whereas three purely avian H5N1 strains were attenuated for replication and provoked higher IFN secretion. Replication of avian viruses was significantly enhanced on interferon-deficient cells, and exogenous IFN potently limited the growth of all strains in human cells. Moreover, IFN-α/β activation by all strains depended on retinoic acid-inducible gene I excluding principal differences in receptor activation between the different viruses. Interestingly, all H5N1 NS1 proteins suppressed IFN-α/β induction comparably well to the NS1 of seasonal IAV. Thus, our study shows that H5N1 strains are heterogeneous in their capacity to activate human cells in an NS1-independent manner. Our findings also suggest that H5N1 viruses need to acquire adaptive changes to circumvent strong IFN-α/β activation in human host cells. Since no single amino acid polymorphism could be associated with a respective high- or low induction phenotype we propose that the necessary adaptations to overcome the human IFN-α/β barrier involve mutations in multiple H5N1 genes.
Additional file 2 of Online peer-led intervention to improve adolescent wellbeing during the COVID-19 pandemic: a randomised controlled trial
Creators: name: Pavarini, Gabriela
name: Reardon, Tessa
name: Mawdsley, Geoffrey
name: Singh, Ilina
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Neuroscience
subject: Physiology
subject: FOS: Biological sciences
subject: Pharmacology
subject: Biotechnology
subject: Sociology
subject: FOS: Sociology
subject: Cancer
subject: Mental Health
subject: Plant Biology

description: Additional file 2. Description of Coping during COVID programme.
Neue Meldepflichten
Creators: name: Wichmann, Ole
Publisher: name: Robert Koch-Institut, Infektionsepidemiologie
Year: 2013
Subjects: subject: Gesetzgebung
subject: Infektionsschutzgesetz
subject: Masern
subject: Meldepflicht
subject: Mumps
subject: Varizellen
subject: 610 Medizin

description: Die Liste der meldepflichtigen Erkrankungen wurde kürzlich um Mumps, Pertussis, Röteln und Varizellen erweitert. Die Gesetzesänderung ermöglicht es, bundesweit verlässliche Daten zur Krankheitslast zu erheben.
Exploring Human Responses to a Virtual Character Bump
Creators: name: Krogmeier, Claudia M
Publisher: name: Purdue University Graduate School
Year: 2019
Subjects: Computer graphics

description: How does haptic feedback during human-virtual character interaction affect participant physiological responses in virtual reality? In this between-subjects study, haptic feedback and non-haptic feedback conditions in which virtual characters bump into the participant who is immersed in a virtual environment are compared. A questionnaire was developed to determine the influence of haptic feedback on presence, embodiment, positive and negative affect, interaction with virtual characters, and haptic feedback realism, among other more exploratory concepts. These exploratory variables include engagement, flow, comfort with virtual characters, comfort with virtual characters’ appearance, realism of virtual character interaction, realism of haptic feedback, and virtual reality sickness. Physiological data was collected using galvanic skin response (GSR) to investigate the influence of haptic feedback on physiological arousal during human-virtual character interaction. Five conditions were developed (no haptic feedback, full and half intensity, incorrect position, and delayed timing). Significant differences were found in embodiment, realism of virtual character interaction, haptic feedback realism, and GSR amplitude after the first interaction with the virtual character. These results may inform future virtual reality studies that investigate haptic feedback during human-virtual character interaction and/or arousal via GSR data, as well as advise studies that seek to correlate self-report responses with physiological data.
Complete Genome Sequence of the English Isolate of Rat Cytomegalovirus (Murid Herpesvirus 8)
Creators: name: Ettinger, Jakob
name: Geyer, Henriette
name: Nitsche, Andreas
name: Zimmermann, Albert
name: Brune, Wolfram
name: Sandford, Gordon R.
name: Hayward, Gary S.
name: Voigt, Sebastian
Publisher: name: Robert Koch-Institut
Year: 2012
Subjects: subject: Animals
subject: Molecular Sequence Data
subject: Rats
subject: Genome Viral
subject: Open Reading Frames
subject: Cytomegalovirus/genetics
subject: 610 Medizin

description: The complete genome of the English isolate of rat cytomegalovirus (RCMV-E) was determined. RCMV-E has a 202,946-bp genome with noninverting repeats but without terminal repeats. Thus, it differs significantly in size and genomic arrangement from closely related rodent cytomegaloviruses (CMVs). To account for the differences between the rat CMV isolates of Maastricht and England, RCMV-E was classified as Murid herpesvirus 8 by the International Committee on Taxonomy of Viruses.
Effect of Bran Particle Size on Gut Microbiota Community Structure and Function
Creators: name: Thakkar, Riya D
Publisher: name: Purdue University Graduate School
Year: 2019
Subjects: Food sciences not elsewhere classified

description: With the advent of industrialization and food processing techniques the sizes of the cereal bran have been drastically reduced. In my thesis, I have tested the effect, if any, of wheat bran and maize bran particle size, in vitro, on the gut microbiota community structure by 16S rRNA sequencing and their function, by Short chain fatty acids (acetate, propionate, butyrate) production. In turn, we also linked the microbiota and SCFA differences to different chemical composition amongst variously sized fractions of wheat and maize bran.
Participant information for MLA 2022 Roundtable “Recreations of Literature and Theatre in Virtual,  Augmented, and Mixed Reality”
Creators: HC User
Publisher: name: Humanities Commons
Year: 2021
Subjects: subject: Virtual reality
subject: Digital Art
subject: Drama
subject: Digital media
subject: Literature

description: Bios and recent work of the participants for the 2022 MLA Roundtable “Recreations of Literature and Theatre in Virtual,  Augmented, and Mixed Reality” scheduled to take place on January 6, 2022.
Proceedings of the International Workshop ‘From Global Burden of Disease Studies to National Burden of Disease Surveillance'
Creators: name: Koch-Institut, Robert
Publisher: name: Robert Koch-Institut, Epidemiologie und Gesundheitsberichterstattung
Year: 2016
Subjects: subject: 610 Medizin

description: The workshop was funded by the Department of Epidemiology and Health Monitoring at Germany’s Robert Koch Institute (RKI) and the German Environment Agency (UBA) in cooperation with the German Society for Epidemiology (DGEpi) e.V. and Bielefeld University, Germany. Publication of this supplement has been fully funded by the Robert Koch Institute, Berlin, Germany.
Participant information for MLA 2022 Roundtable "Recreations of Literature and Theatre in Virtual, Augmented, and Mixed Reality"
Creators: name: Bushnell, Rebecca
name: Gorman, Samantha
name: Hunter, Elizabeth
name: Leichman, Jeffrey
name: Reside, Doug
name: Szabo, Victoria
Publisher: name: unknown
Year: 2021
Subjects: subject: Digital media
subject: Virtual reality
subject: Drama
subject: Literature
subject: Digital art

description: Bios and recent work of the participants for the 2022 MLA Roundtable "Recreations of Literature and Theatre in Virtual, Augmented, and Mixed Reality" scheduled to take place on January 6, 2022.
LIFE CYCLE COST ANALYSIS OF AN ENERGY EFFICIENT RESIDENTIAL UNIT
Creators: name: Hajare, Ayushi
Publisher: name: Purdue University Graduate School
Year: 2019
Subjects: Building construction management and project planning

description: The residential building sector is one of the most energy intensive sectors in today’s civilization. With population growth and a rise in number of homeowners the effect is bound to worsen. A wave of green and sustainable strategies is on the rise hoping to moderate some of the negative effect on the environment. From embracing renewable sources of energy as an alternative to fossil fuels, to improving existing home systems to become more efficient, the construction industry is evolving into becoming more energy conscious. One of the biggest obstacles to this wave is a lack of awareness and a fear of initial costs among contractors, homeowners and clients alike. This research will use Life Cycle Cost Analysis on a varying combination of residential energy systems and the researcher hopes to establish the trade-off between initial investment and long-term benefits. The case being considered is a residence located in Indiana, US. Using past and current utility bills and energy simulation data of different energy consuming systems in the residence over its lifetime, economic models are generated. This research establishes that a combination of passive and active energy conservation measures results in the lowest life cycle cost. The study will be beneficial for further research and as a framework for residential life cycle cost analysis.
Effectiveness of Pneumococcal Conjugate Vaccines (PCV7 and PCV13) against Invasive Pneumococcal Disease among Children under Two Years of Age in Germany
Creators: name: Linden, Mark Van Der
name: Falkenhorst, Gerhard
name: Perniciaro, Stephanie
name: Fitzner, Christina
name: Imöhl, Matthias
Publisher: name: Robert Koch-Institut, Infektionsepidemiologie
Year: 2016
Subjects: subject: 610 Medizin

description: Background: In this study we calculate the effectiveness of pneumococcal conjugate vaccines (PCV) against invasive pneumococcal disease (IPD) among children under the age of two years using the indirect cohort method. We also discuss the timeliness of vaccination and the residual cases of vaccine type IPD. Methods and Findings: From July 2006 until June 2015, 921 IPD cases were reported and for 618 children (67.1%), the vaccination status at the time of infection could be accurately determined. Of these, 379 (61.3%) were vaccinated and 239 (38.7%) were not vaccinated. The adjusted vaccine effectiveness (VE) of PCV7 for all included serotypes + 6A was 80% (95% CI: 63–89) for at least one dose, 97% (89–100) after three primary doses (post primary) and 95% (57–100) post booster. The adjusted overall VE of PCV13 was 86% (74–93) for at least one dose, 85% (62–94) post primary and 91% (61–99) post booster. For the additional serotypes included in PCV13, the adjusted VE was 82% (66–91), 80% (46–93) and 90% (54–98) respectively. The serotype specific VE for at least one dose was high for serotypes 1 (83%; 15–97), 3 (74%; 2–93), 7F (84%; 18–98) and 19A (77%; 47–90). Only 39.5% of children with IPD obtained their first dose of PCV7 according to schedule (2nd dose: 32.9%, 3rd dose: 22.0%, booster dose: 63.6%). For children vaccinated with PCV13 values were slightly better: 43.8%, 33.5%, 26.3% and 74.3% respectively. Among 90 residual cases with PCV7 serotypes, 73 (81.1%) were in unvaccinated children, and 15 (16.7%) in children who had not obtained the number of doses recommended for their age, and only two (2.2%) in children vaccinated according to age. Of 82 cases with PCV13 serotypes occurring after the switch from PCV7 to PCV13, 56 (68.3%) were not vaccinated, 22 (26.8%) were incompletely vaccinated, and four (4.9%) were vaccinated according to age. Conclusions: Our data show a high effectiveness of pneumococcal conjugate vaccination in Germany. However, the administration of vaccine doses among children with IPD is often delayed, resulting in many vaccine type cases in non- or incompletely-vaccinated children. Whether the recently-implemented change to a 2+1 schedule will improve the timeliness of vaccination should be subject to careful monitoring.
Additional file 1 of Engineering Escherichia coli for high-yielding 2,5-Dimethylpyrazine synthesis from L-Threonine by reconstructing metabolic pathways and enhancing cofactors regeneration
Creators: name: Liu, Xin-Xin
name: Wang, Yao
name: Zhang, Jian-Hui
name: Lu, Yun-Feng
name: Dong, Zi-Xing
name: Yue, Chao
name: Huang, Xian-Qing
name: Zhang, Si-Pu
name: Li, Dan-Dan
name: Yao, Lun-Guang
name: Tang, Cun-Duo
Publisher: name: figshare
Year: 2024
Subjects: subject: Microbiology
subject: FOS: Biological sciences

description: Additional file 1: Figure S1. The agarose gel electrophoresis analysis for PCR products of EcTDH and double enzyme digestion verification of recombinant E. coli BL21(DE3)/pACYCDuet-1-Ectdh. Figure S2. The agarose gel electrophoresis analysis for PCR products of EhNOX and double enzyme digestion verification of recombinant E. coli BL21(DE3)/pACYCDuet-1-Ectdh-Ehnox. Figure S3. Analysis of PCR products of vector pETDuet-1 by agarose gel electrophoresis. Figure S4. The agarose gel electrophoresis analysis for PCR products of EcSstT and ScAAO and universal primer validation of recombinant E. coli BL21(DE3)/pETDuet-1-Scaao-Ecsstt. Figure S5. The universal primer validation of recombinant E. coli BL21(DE3)/pACYCDuet-1-Ectdh-Ehnox: pETDuet-1-Scaao-Ecsstt. Figure S6. Correlation analysis of 2,5-DMP with peak area. Figure S7A. HPLC analysis of substrate L- threonine standard. B HPLC analysis of 2,5-DMP. Figure S8. HPLC analysis catalytic products. Figure S9A. NMR analysis of products in fermentation broths. B MS analysis of products in fermentation broths. Figure S10A. NMR analysis of substrates in fermentation broths. B MS analysis of substrates in fermentation broths. Figure S11. The results in Figure 4 were analyzed for significance of differences. Figure S12. The results in Figure 5 were analyzed for significance of differences. Figure S13. The results in Figure 6 were analyzed for significance of differences. Figure S14. The results in Figure 7 were analyzed for significance of differences. Table S1. Strains and plasmids used in this study. Table S2. Primers used in this study. Table S3. HPLC gradient elution of the product 2,5-DMP.
Nexus Genesis Synesthesia - NGS Systems: The Future of Intelligently Managed Innovation
Creators: name: Billions, Ava
name: Knight, Chris
Publisher: name: Zenodo
Year: 2024

description: Abstract

In an era of rapid technological advancement, managing innovation has become increasingly complex. Nexus Genesis Synesthesia (NGS Systems) emerges as a groundbreaking open-source platform designed to address this challenge. NGS Systems integrates a diverse array of tools and technologies, including a sophisticated TRL (Technology Readiness Level) management framework, a powerful Eclipse-like IDE, cutting-edge AI capabilities, and immersive XR integration. This paper explores the origins, core components, and future directions of NGS Systems, highlighting its potential to revolutionize technology development and usher in a new era of intelligently managed innovation. By fostering collaboration, streamlining workflows, and empowering creators with advanced tools, NGS Systems paves the way for a future where innovation flourishes and technology serves humanity's greatest aspirations.
Folate status in Germany
Creators: name: Mensink, Gert
name: Weißenborn, Anke
name: Richter, Almut
Publisher: name: Robert Koch-Institut
Year: 2016
Subjects: subject: Germany
subject: health survey
subject: DEGS 1
subject: nutrition
subject: folate
subject: 610 Medizin

description: Folate is important for cell division and thus for growth and physical development. Serum folate concentrations can be used to assess a population’s folate status. In Germany, the median serum folate level of adults aged 18 to 79 is 7.5 ng/ml (women: 7.9 ng/ml, and men: 7.2 ng/ml). Approximately 86% of the adult population in Germany has adequate folate levels. Higher folate concentrations are observed among older age groups and individuals with a higher socio-economic status. However, the World Health Organization recommends that women of reproductive age should, at the population level, have significantly higher folate levels in order to reduce the risk for neural tube defects. However, the majority of women in this age group do not achieve these concentrations.
Predicting 10-Year Risk of Fatal Cardiovascular Disease in Germany: An Update Based on the SCORE-Deutschland Risk Charts
Creators: name: Rücker, Viktoria
name: Keil, Ulrich
name: Fitzgerald, Anthony P.
name: Malzahn, Uwe
name: Prugger, Christof
name: Ertl, Georg
name: Heuschmann, Peter U.
name: Neuhauser, Hannelore
Publisher: name: Robert Koch-Institut, Epidemiologie und Gesundheitsberichterstattung
Year: 2016
Subjects: subject: 610 Medizin

description: Estimation of absolute risk of cardiovascular disease (CVD), preferably with population-specific risk charts, has become a cornerstone of CVD primary prevention. Regular recalibration of risk charts may be necessary due to decreasing CVD rates and CVD risk factor levels. The SCORE risk charts for fatal CVD risk assessment were first calibrated for Germany with 1998 risk factor level data and 1999 mortality statistics. We present an update of these risk charts based on the SCORE methodology including estimates of relative risks from SCORE, risk factor levels from the German Health Interview and Examination Survey for Adults 2008–11 (DEGS1) and official mortality statistics from 2012. Competing risks methods were applied and estimates were independently validated. Updated risk charts were calculated based on cholesterol, smoking, systolic blood pressure risk factor levels, sex and 5-year age-groups. The absolute 10-year risk estimates of fatal CVD were lower according to the updated risk charts compared to the first calibration for Germany. In a nationwide sample of 3062 adults aged 40–65 years free of major CVD from DEGS1, the mean 10-year risk of fatal CVD estimated by the updated charts was lower by 29% and the estimated proportion of high risk people (10-year risk > = 5%) by 50% compared to the older risk charts. This recalibration shows a need for regular updates of risk charts according to changes in mortality and risk factor levels in order to sustain the identification of people with a high CVD risk.
Bluthochdruck in Deutschland
Creators: name: Neuhauser, Hannelore
name: Diederichs, Claudia
name: Boeing, Heiner
name: Felix, Stephan B.
name: Jünger, Claus
name: Lorbeer, Roberto
name: Meisinger, Christine
name: Peters, Annette
name: Völzke, Henry
name: Weikert, Cornelia
name: Wild, Philipp
name: Dörr, Marcus
Publisher: name: Robert Koch-Institut, Epidemiologie und Gesundheitsberichterstattung
Year: 2016
Subjects: subject: 610 Medizin

description: Hintergrund: Bluthochdruck ist ein zentraler Risikofaktor. In Deutschland gibt es wenige bevölkerungsbezogene Datenerhebungen, die auf standardisierten Blutdruckmessungen beruhen. Methode: Es wurden standardisierte Blutdruckmessungen und Medikamentendaten aus 7 bevölkerungsbasierten Studien mit insgesamt 66 845 Teilnehmenden im Alter von 25–74 Jahren analysiert. Diese Studien waren in Deutschland zwischen 1994 und 2012 durchgeführt worden. Die EPIC-Potsdam-Studie (1994–1998), die KORA-S4-Studie (1999–2001) in Augsburg sowie die Gutenberg Health Study (2007–2012) in und um Mainz wurden für deskriptive Vergleiche herangezogen. Aufgrund gleicher Studienregionen konnten der Bundes-Gesundheitssurvey 1998 mit der Studie zur Gesundheit Erwachsener in Deutschland (2008–2011) sowie die Study of Health in Pomerania (SHIP) in Nordost-Deutschland (1997–2001) mit der SHIP-TREND-Studie (2008–2012) im direkten zeitlichen Vergleich analysiert werden. Alle Blutdruckdaten wurden für gerätespezifische Messwertunterschiede adjustiert. Ergebnisse: Nach Anpassung von studienspezifischen Messgeräteunterschieden waren die mittleren systolischen und diastolischen Blutdruckwerte in den neueren Studien (2007–2012) niedriger als in den älteren Studien (1994–2001); der Anteil behandelter Hypertoniker nahm zu. Den stärksten Rückgang beim mittleren Blutdruckwert (systolisch ≥ 10 mm Hg) gab es bei den 55- bis 74-Jährigen. Bei jungen Männern zwischen 25 und 34 Jahren wurde auf der Grundlage der regionalen SHIP-TREND-Daten ebenfalls ein Rückgang des mittleren systolischen Blutdruckwerts verzeichnet, der sich jedoch in den bundesweit erhobenen DEGS1-Daten nicht widerspiegelte. Schlussfolgerung: Trotz der guten Evidenz für abnehmende Blutdruckwerte in Deutschland in den vergangenen zwei Jahrzehnten bleibt das Präventionspotenzial hoch. Die Aktualisierung der bevölkerungsbezogenen Daten mit besonderem Fokus auf die Blutdruckdaten junger Männer sollte Ziel künftiger Forschungsbemühungen sein.
PaPrBaG: A machine learning approach for the detection of novel pathogens from NGS data
Creators: name: Deneke, Carlus
name: Rentzsch, Robert
name: Renard, Bernhard Y.
Publisher: name: Robert Koch-Institut
Year: 2017
Subjects: subject: 610 Medizin

description: The reliable detection of novel bacterial pathogens from next-generation sequencing data is a key challenge for microbial diagnostics. Current computational tools usually rely on sequence similarity and often fail to detect novel species when closely related genomes are unavailable or missing from the reference database. Here we present the machine learning based approach PaPrBaG (Pathogenicity Prediction for Bacterial Genomes). PaPrBaG overcomes genetic divergence by training on a wide range of species with known pathogenicity phenotype. To that end we compiled a comprehensive list of pathogenic and non-pathogenic bacteria with human host, using various genome metadata in conjunction with a rule-based protocol. A detailed comparative study reveals that PaPrBaG has several advantages over sequence similarity approaches. Most importantly, it always provides a prediction whereas other approaches discard a large number of sequencing reads with low similarity to currently known reference genomes. Furthermore, PaPrBaG remains reliable even at very low genomic coverages. CombiningPaPrBaG with existing approaches further improves prediction results.