title: Ð ÐµÐ´Ð¾ÐºÑ-Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¼ Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð¿ÑÐ¸ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ð¸ ÑÑÑÐµÑÑ-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Redox metabolism of human mesenchymal stem cells during the development of stress-induced aging
Creators: name: ÐÐ½ÑÐ¾Ð½Ð¾Ð², Ð¡ÐµÐ¼ÑÐ½
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸-ÐºÐ¾Ð´Ð¸ÑÑÐµÐ¼ÑÐµ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½ÑÐµ Ð±Ð¸Ð¾ÑÐµÐ½ÑÐ¾ÑÑ
Ð°ÐºÑÐ¸Ð²Ð½ÑÐµ ÑÐ¾ÑÐ¼Ñ ÐºÐ¸ÑÐ»Ð¾ÑÐ¾Ð´Ð°
ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ð°Ð»ÑÐ½ÑÐµ Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÐµ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸
ÑÑÑÐµÑÑ-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ðµ ÑÑÐ°ÑÐµÐ½Ð¸Ðµ
ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ðµ ÑÑÐ°ÑÐµÐ½Ð¸Ðµ
Ð¾ÐºÐ¸ÑÐ»Ð¸ÑÐµÐ»ÑÐ½ÑÐ¹ ÑÑÑÐµÑÑ
genetic-encoded fluorescent biosensors
active oxygen species
endometrial mesenchymal stem cells
stress-induced aging
replicative aging
oxidizing agents

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«Ð ÐµÐ´Ð¾ÐºÑ-Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¼ Ð¼ÐµÐ·ÐµÐ½ÑÐ¸Ð¼Ð½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð¿ÑÐ¸ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ð¸ ÑÑÑÐµÑÑ-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸ÑÂ». Ð Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ Ð¿ÑÐ¾Ð°Ð½Ð°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°Ð½Ð¾ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ Ð¿ÐµÑÐµÐºÐ¸ÑÐ¸ Ð²Ð¾Ð´Ð¾ÑÐ¾Ð´Ð° Ð² Ð¼ÐµÐ·ÐµÐ½ÑÐ¸Ð¼Ð½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ñ (ÐÐ¡ÐÐ­) ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð¿ÑÐ¸ Ð¸Ð½Ð´ÑÐºÑÐ¸Ð¸ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ. ÐÐµÑÐ¾Ð´Ð¸ÑÐµÑÐºÐ°Ñ Ð¾ÑÐ½Ð¾Ð²Ð° ÑÐ°Ð±Ð¾ÑÑ â Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸ ÐºÐ¾Ð´Ð¸ÑÑÐµÐ¼Ð¾Ð³Ð¾ Ð±Ð¸Ð¾ÑÐµÐ½ÑÐ¾ÑÐ° Ð¿ÐµÑÐµÐºÐ¸ÑÐ¸ Ð²Ð¾Ð´Ð¾ÑÐ¾Ð´Ð° HyPer, Ð° ÑÐ°ÐºÐ¶Ðµ ÑÐ-Ð±Ð¸Ð¾ÑÐµÐ½ÑÐ¾ÑÐ° SypHer. ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: 1. Ð¢ÑÐ°Ð½ÑÐ´ÑÑÐ¸ÑÐ¾Ð²Ð°ÑÑ ÐÐ¡ÐÐ­ Ð»ÐµÐ½ÑÐ¸Ð²Ð¸ÑÑÑÐ°Ð¼Ð¸ Ð´Ð»Ñ Ð³ÐµÐ½ÐµÑÐ°ÑÐ¸Ð¸ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð»Ð¸Ð½Ð¸Ð¹, ÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾-ÑÐºÑÐ¿ÑÐµÑÑÐ¸ÑÑÑÑÐ¸Ñ Ð±Ð¸Ð¾ÑÐµÐ½ÑÐ¾ÑÑ HyPer Ð¸ SypHer Ð² ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐ¸ÑÐ¾Ð¿Ð»Ð°Ð·Ð¼Ðµ. 2. Ð¡ÑÐ°Ð²Ð½Ð¸ÑÑ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ¸ ÐºÐ»ÐµÑÐ¾Ðº, ÑÐºÑÐ¿ÑÐµÑÑÐ¸ÑÑÑÑÐ¸Ñ Ð±Ð¸Ð¾ÑÐµÐ½ÑÐ¾Ñ HyPer, Ð¿ÑÐ¸ ÑÑÑÐµÑÑ-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð¼ Ð¸ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ð¼ ÑÑÐ°ÑÐµÐ½Ð¸Ð¸. 3. ÐÑÐ¾Ð²ÐµÑÑÐ¸ Ð¾ÑÐµÐ½ÐºÑ ÑÐµÐ´Ð¾ÐºÑ-ÑÐ¾ÑÑÐ¾ÑÐ½Ð¸Ñ Ð¿Ð¾ÑÑÐ°ÑÐµÐ²ÑÐ¸Ñ ÐºÐ»ÐµÑÐ¾Ðº. Ð Ð°Ð±Ð¾ÑÐ° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ Ð¤ÐÐÐ£ "ÐÐ½ÑÑÐ¸ÑÑÑ ÑÐ¸ÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð ÐÐ". Ð ÑÐ°Ð±Ð¾ÑÐµ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð²ÑÐµ Ð¾ÑÐ½Ð¾Ð²Ð½ÑÐµ Ð¿ÑÐ¸Ð·Ð½Ð°ÐºÐ¸ ÑÑÐ°ÑÐµÐ½Ð¸Ñ, Ð¸Ð½Ð´ÑÑÐ¸ÑÑÐµÐ¼ÑÐµ Ð¾ÐºÐ¸ÑÐ»Ð¸ÑÐµÐ»ÑÐ½ÑÐ¼ ÑÑÑÐµÑÑÐ¾Ð¼ Ð² ÐºÑÐ»ÑÑÑÑÐ°Ñ ÐÐ¡ÐÐ­, ÑÐºÑÐ¿ÑÐµÑÑÐ¸ÑÑÑÑÐ¸Ñ HyPer, ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²ÑÑÑ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ°Ð¼ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾ ÑÐ¾ÑÑÐ°ÑÐµÐ½Ð½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº. ÐÐ±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¾, ÑÑÐ¾ Ð¿ÑÐ¸ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ð¸ ÑÑÑÐµÑÑ-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ð¸ ÐºÐ»ÐµÑÐ¾Ðº Ð½Ð°Ð±Ð»ÑÐ´Ð°ÐµÑÑÑ ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ðµ ÑÑÐ¾Ð²Ð½Ñ Ð°ÐºÑÐ¸Ð²Ð½ÑÑ ÑÐ¾ÑÐ¼ ÐºÐ¸ÑÐ»Ð¾ÑÐ¾Ð´Ð° Ð¸ Ð·Ð°ÐºÐ¸ÑÐ»ÐµÐ½Ð¸Ðµ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐ¸ÑÐ¾Ð¿Ð»Ð°Ð·Ð¼Ñ, Ð¾Ð´Ð½Ð°ÐºÐ¾, ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ñ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ Ð¿ÐµÑÐµÐºÐ¸ÑÐ¸ Ð²Ð¾Ð´Ð¾ÑÐ¾Ð´Ð° Ð² ÑÐ¸ÑÐ¾Ð¿Ð»Ð°Ð·Ð¼Ðµ ÐºÐ»ÐµÑÐ¾Ðº Ð½Ðµ Ð¿ÑÐ¾Ð¸ÑÑÐ¾Ð´Ð¸Ñ. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ Ð´Ð°Ð½Ð½ÑÐµ Ð¼Ð¾Ð³ÑÑ Ð¸Ð¼ÐµÑÑ ÑÑÐ½Ð´Ð°Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½Ð¾Ðµ Ð·Ð½Ð°ÑÐµÐ½Ð¸Ðµ Ð´Ð»Ñ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ñ ÑÐµÐ¾ÑÐ¸Ð¹ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ.
description: The theme of the final qualification work: "Redox metabolism of human mesenchymal stem cells during the development of stress-induced aging." In this paper, we analyzed the change in the concentration of intracellular hydrogen peroxide in human endometrial mesenchymal stem cells (MSCE) during the induction of cellular aging. The methodological basis of the work is the use of the genetically encoded biosensor of hydrogen peroxide HyPer, as well as the pH biosensor SypHer. Tasks that were solved during the study: 1. Transduce MSCE with lentiviruses to generate cell lines stably expressing HyPer and SypHer biosensors in the cell cytoplasm. 2. Compare the characteristics of cells expressing biosensors in stress-induced and replicative aging. 3. Assess the redox state of the aged cells. The work was carried out based on the Institute of Cytology RAS. It was shown that all the main signs of aging-induced by oxidative stress in MSCE cultures expressing HyPer correspond to the characteristics of replicative aged cells. It was found that with the development of stress-induced cell aging, an increase in the level of reactive oxygen species and acidification of the cell cytoplasm is observed, however, an increase in the concentration of hydrogen peroxide in the cell cytoplasm does not occur. The findings may be fundamental to the development of theories of cell aging.