Additional file 1 of In vitro magnetosome remineralization for silver-magnetite hybrid magnetosome biosynthesis and used for healing of the infected wound
Creators: name: Xu, Junjie
name: Ma, Shijiao
name: Zhang, Wei
name: Jia, Lina
name: Zheng, Haolan
name: Bo, Pang
name: Bai, Xue
name: Sun, Hongyan
name: Qi, Lei
name: Zhang, Tongwei
name: Chen, Chuanfang
name: Li, Feng
name: Arai, Fumihito
name: Tian, Jiesheng
name: Feng, Lin
Publisher: name: figshare
Year: 2022
Subjects: subject: Biochemistry
subject: Space Science
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Pharmacology
subject: Chemical Sciences not elsewhere classified
subject: Cancer
subject: Infectious Diseases
subject: FOS: Health sciences

description: Additional file 1: Figure S1. Ag nanoparticle diameter statistical distribution of Ag-Fe3O4 hybrid BMPs synthesis at room temperature and 80°C. The average diameter of Ag nanoparticles was 9.85±2.22 nm and 17.51±3.14 nm under room temperature and 80℃, which increased almost two fold. Figure S2. Functional groups and crystal lattice analysis of BMP-Ag synthesized under AgNO3 concentration 1.0 mg/mL, reaction time 1 min, reaction temperature 80°C, and room temperature by FTIR and XRD. The functional groups of BMP-Ag no obvious change under different synthesized parameters. There is a faint diffraction peak at 38.1o of BMP-Ag responding to the (111) crystal plane of face-centered cubic Ag compared with BMP, and the full width at half maximum decreased as the Ag NP size increased at a reaction temperature of 80 °C. Figure S3. (A) Magnetotactic bacteria MSR-1 cultured at different concentrations of AgNO3 solution. The bacterial growth was inhibited when the AgNO3 concentrations &gt;1.0 mg/mL. (B) Transmission electron microscopy image of MSR-1 cultured under 1.0 mg/mL AgNO3 solution. There is no synthesis of Ag-Fe3O4 hybrid BMP. Figure S4. Transmission electron microscopy image of chemically synthesized Fe3O4(A), commercial biotinylated magnetic beads(B), and streptavidin magnetic beads(C) and BMPs(D) after incubating with AgNO3 solution. Only BMP can mineralize Ag+ into Ag nanoparticles. Figure S5. Construction of MGMSRv2_1436 gene deletion mutant. (A) Schematic diagram of the construction of suicide vector pUXcusA. (B) Amplified upstream and downstream fragments of MGMSRv2_1436 gene and gentamicin resistance cassette gene. (C) Polymerase chain reaction (PCR) amplified the internal gene of MGMSRv2_1436 (1436), gentamicin resistance gene (Gm), upstream and downstream fragments of MGMSRv2_1436 gene to confirm the screened MGMSRv2_1436 mutant strain. #: MGMSRv2_1436 mutant strain, + WT MSR-1 positive control, - ddH2O negative control. There was no MGMSRv2_1436 gene, but has gentamicin resistance gene, upstream, and downstream fragments in the screened colonies, mean MGMSRv2_1436 deletion mutant was obtained successfully. (D) PCR amplified the genes (mamB, mamD, mamE, mamO, mamX, mamZ, mms6, feoB) associated with the magnetosome synthesis. It was confirmed that no genes were lost in the construction process. Figure S6. Transmission electron microscopy image of MSR-Δ1436 BMP after incubating with AgNO3 solution. MSR-Δ1436 BMP still can mineralize Ag+ into Ag nanoparticles; it was indicated that Ag+ transported into BMP membrane did not base on efflux pump MGMSRv2_1436 protein in vitro state. Figure S7. Transmission electron microscopy image of Fe3O4 crystal and vesicle(B) of BMP after incubating with AgNO3 solution. There was no Ag nanoparticle was synthesis on those two components. It was indicated that remineralization of BMP needs synergy of each element of BMP. Table S1. Proteins of only exist on BMP membrane.