Additional file 1 of Nanofiber-mediated sequential photothermal antibacteria and macrophage polarization for healing MRSA-infected diabetic wounds
Creators: name: Xu, Zhou
name: Deng, Bin
name: Wang, Xuewen
name: Yu, Jie
name: Xu, Zhuobin
name: Liu, Penggang
name: Liu, Caihong
name: Cai, Yuan
name: Wang, Fei
name: Zong, Rongling
name: Chen, Zhiling
name: Xing, Hua
name: Chen, Gang
Publisher: name: figshare
Year: 2021
Subjects: subject: Space Science
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Plant Biology
subject: Virology

description: Additional file 1: Fig. S1. UV–Vis-NIR spectra of Cur, PDA NTs, PDA/Cur NFs, and PDA/Cur NFs + NIR. Fig. S2. (a) Thermal image of PDA NTs under NIR laser (808 nm, 0.8 W/cm2) for 300 s. (b) Temperature elevation curves of PDA NTs at different concentrations (0–180 μg/mL) upon NIR laser (808 nm, 0.8 W/cm2) for 300 s. (c) Plot of temperature change (ΔT) over a period of 300 s versus the concentration of PDA NTs. (d) Photothermal conversion ability of PDA NTs during 5 times of on/off NIR irradiation cycles. (e) Photothermal response of PDA NTs (180 μg/mL) for 300 s with NIR laser (808 nm, 0.8 W/cm2) and then the laser was shut off. Fig. S3. Standard curve of Cur measured by HPLC. Fig. S4. (a) Antibacterial effects of Cur nanocrystals, PDA NTs, and PDA/Cur NFs at different concentrations for 30 min incubation. (b) Photographs of bacterial colonies of MRSA after being treated with Cur nanocrystals, PDA NTs, and PDA/Cur NFs for 30 min at different concentrations. Fig. S5. (a) Antibacterial effects of Cur nanocrystals (10 μg/mL), PDA NTs (90 μg/mL), and PDA/Cur NFs (100 μg/mL) for different incubation times. (b) Photographs of bacterial colonies of MRSA after being treated with Cur nanocrystals (10 μg/mL), PDA NTs (90 μg/mL), and PDA/Cur NFs (100 μg/mL) for different incubation times. Fig. S6. (a) Photographs of bacterial colonies of MRSA after being incubated with Cur nanocrystals and PDA NTs at different concentrations under NIR laser (808 nm, 0.8 W/cm2, 300 s). (b) Photographs of bacterial colonies of MRSA after being treated with Cur nanocrystals (10 μg/mL) and PDA NTs (90 μg/mL) under NIR laser (808 nm, 0.8 W/cm2) for different times. Fig. S7. (a) The cell viability of L929 cell after being treated with Cur nanocrystals, PDA NTs, PDA/Cur NFs, and PDA/Cur NFs + NIR at 24 h post-incubation with different concentrations. (b–c) The crystal violet staining and relative absorbance of L929 cell after being treated with PDA NTs at 24 h post-incubation with different concentrations (scale bar = 50 μm). Fig. S8. (a) The cell viability of RAW264.7 cell after being treated with Cur nanocrystals, PDA NTs, PDA/Cur NFs, and PDA/Cur NFs + NIR at 24 h post-incubation with different concentrations. (b–c) The crystal violet staining and relative absorbance of RAW264.7 cell after being treated with PDA NTs at 24 h post-incubation with different concentrations (scale bar = 50 μm). Fig. S9. A schematic illustration showing the in vivo infected diabetic wounds at different time intervals under treatment. Fig. S10. H&amp;E staining of wounds treated with saline + NIR, PDA NTs, and PDA/Cur NFs on days 3, 7, and 14. Black arrows indicated the wound boundaries. Fig. S11. Masson staining of wounds treated with saline + NIR, PDA NTs, and PDA/Cur NFs on days 3, 7, and 14. Fig. S12. (a) Representative immunofluorescence images showing Ki67 for different groups. (b) The relative quantification of Ki67 after different treatments. Data are presented as mean ± SD (n = 3). **P &lt; 0.01, ***P &lt; 0.001, ****P &lt; 0.0001, vs saline group. ##P &lt; 0.01, ###P &lt; 0.001, vs the indicated groups.