title: ÐÐ¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° ÑÐºÐ´Ð¸ÑÑÐµÑÐ¾Ð½Ð° Ð² ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð¼Ð¾Ð´ÐµÐ»ÑÑ ÐºÐ°ÑÑÐ¸Ð½Ð¾Ð¼Ñ Ð»ÐµÐ³ÐºÐ¾Ð³Ð¾
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Biological properties of ecdysterone in cell models of lung carcinoma
Creators: name: ÐÐ¸ÑÐ´ÐµÐµÐ²Ð°, Ð®Ð»Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÐÐ¸Ð¾ÑÐµÑ Ð½Ð¾Ð»Ð¾Ð³Ð¸Ñ
ÑÐºÐ´Ð¸ÑÑÐµÑÐ¾Ð½
ÑÐ°Ðº Ð»ÐµÐ³ÐºÐ¸Ñ
Ð´Ð¾ÐºÑÐ¾ÑÑÐ±Ð¸ÑÐ¸Ð½
Ð°ÑÑÐ¾ÑÐ°Ð³Ð¸Ñ
ecdysterone
lung cancer
doxorubicin
autophagy

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ ÑÐ²Ð¾Ð¹ÑÑÐ² Â«ÑÐ¸ÑÑÐ¾Ð³Ð¾Â» (â¥97%) ÑÐºÐ´Ð¸ÑÑÐµÑÐ¾Ð½Ð°, Ð° ÑÐ°ÐºÐ¶Ðµ ÐµÐ³Ð¾ ÐºÐ¾Ð¼Ð¼ÐµÑÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐºÑÑÑÐ°ÐºÑÐ° Ð¸Ð· ÑÐ¸Ð°Ð½Ð¾ÑÐ¸ÑÐ° Â«Ð¥Â®Â» (ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ðµ ÑÐºÐ´Ð¸ÑÑÐµÑÐ¾Ð½Ð° 18%) Ð² ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð¼Ð¾Ð´ÐµÐ»ÑÑ ÐºÐ°ÑÑÐ¸Ð½Ð¾Ð¼Ñ Ð»ÐµÐ³ÐºÐ¾Ð³Ð¾. Ð ÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð¿Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÑÑ, ÑÑÐ¾ ÑÐºÐ´Ð¸ÑÑÐµÑÐ¾Ð½ Ð²ÑÑÐ¾ÐºÐ¾Ð¹ ÑÑÐµÐ¿ÐµÐ½Ð¸ Ð¾ÑÐ¸ÑÑÐºÐ¸ Ð¸ ÐºÐ¾Ð¼Ð¼ÐµÑÑÐµÑÐºÐ¸Ð¹ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¹ ÑÐºÑÑÑÐ°ÐºÑ Ð¸Ð· ÑÐ¸Ð°Ð½Ð¾ÑÐ¸ÑÐ° Ð½Ðµ Ð²Ð»Ð¸ÑÑÑ Ð½Ð° Ð¿ÑÐ¾Ð»Ð¸ÑÐµÑÐ°ÑÐ¸Ñ ÐºÐ»ÐµÑÐ¾Ðº Ð°Ð´ÐµÐ½Ð¾ÐºÐ°ÑÑÐ¸Ð½Ð¾Ð¼Ñ. ÐÑÐ¸ ÑÑÐ¾Ð¼ ÐºÐ¾Ð¼Ð¼ÐµÑÑÐµÑÐºÐ¸Ð¹ ÑÐºÑÑÑÐ°ÐºÑ, Ð² Ð¾ÑÐ»Ð¸ÑÐ¸Ðµ Ð¾Ñ Ð²ÑÑÐ¾ÐºÐ¾Ð¾ÑÐ¸ÑÐµÐ½Ð½Ð¾Ð³Ð¾, ÑÐµÐ½ÑÐ¸Ð±Ð¸Ð»Ð¸Ð·Ð¸ÑÑÐµÑ ÐºÐ°Ðº Ð¾Ð¿ÑÑÐ¾Ð»ÐµÐ²ÑÐµ, ÑÐ°Ðº Ð¸ Ð½Ð¾ÑÐ¼Ð°Ð»ÑÐ½ÑÐµ ÐºÐ»ÐµÑÐºÐ¸ Ðº ÑÐµÑÐ°Ð¿ÐµÐ²ÑÐ¸ÑÐµÑÐºÐ¾Ð¼Ñ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÑ Ð´Ð¾ÐºÑÐ¾ÑÑÐ±Ð¸ÑÐ¸Ð½Ñ, ÑÑÐ¾ Ð²ÑÐ·ÑÐ²Ð°ÐµÑÐ¾Ð¿Ð°ÑÐµÐ½Ð¸Ñ Ð¿ÑÐ¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ð¸ Ð¸Ð¼ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ¾Ð¼. Ð ÑÐ¾ Ð¶Ðµ Ð²ÑÐµÐ¼Ñ ÐºÐ¾Ð¼Ð¼ÐµÑÑÐµÑÐºÐ¸Ð¹ ÑÐºÐ´Ð¸ÑÑÐµÑÐ¾Ð½ Ð¸Ð· ÑÐ¸Ð°Ð½Ð¾ÑÐ¸ÑÐ° Ð½Ðµ Ð¿ÑÐ¾ÑÐ²Ð¸Ð» ÑÐ¸ÑÐ¾ÑÐ¾ÐºÑÐ¸ÑÐ½Ð¾ÑÑÑ Ðº Ð½Ð¾ÑÐ¼Ð°Ð»ÑÐ½ÑÐ¼ ÐºÐ»ÐµÑÐºÐ°Ð¼ Ð² Ð¾Ð±ÑÑÐ½ÑÑ ÑÑÐ»Ð¾Ð²Ð¸ÑÑ. ÐÑÑÐ¾ÐºÐ¾Ð¾ÑÐ¸ÑÐµÐ½Ð½ÑÐ¹ Ð¸ ÐºÐ¾Ð¼Ð¼ÐµÑÑÐµÑÐºÐ¸Ð¹ ÑÐºÑÑÑÐ°ÐºÑÑ Ð¿Ð¾Ð²ÑÑÐ°Ð»Ð¸ ÑÑÐ¾Ð²ÐµÐ½Ñ ÐÐ¤Ð, Ð²ÑÐ·ÑÐ²Ð°Ð»Ð¸ Ð¿ÑÐ¾ÑÐ¸Ð²Ð¾Ð¿Ð¾Ð»Ð¾Ð¶Ð½ÑÐµ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ð½Ð¾Ð³Ð¾ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð° Ð¼Ð¸ÑÐ¾ÑÐ¾Ð½Ð´ÑÐ¸Ð¹ Ð¸ Ð¿Ð¾-ÑÐ°Ð·Ð½Ð¾Ð¼Ñ Ð²Ð¾Ð·Ð´ÐµÐ¹ÑÑÐ²Ð¾Ð²Ð°Ð»Ð¸ Ð½Ð° Ð¿ÑÐ¾ÑÐµÑÑ Ð°ÑÑÐ¾ÑÐ°Ð³Ð¸Ð¸. Ð¡ÐºÐ¾ÑÐµÐµ Ð²ÑÐµÐ³Ð¾, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð¾Ð±ÑÑÑÐ½ÑÑÑÑÑ Ð½Ð°Ð»Ð¸ÑÐ¸ÐµÐ¼ Ð² ÐºÐ¾Ð¼Ð¼ÐµÑÑÐµÑÐºÐ¾Ð¼ ÑÐºÑÑÑÐ°ÐºÑÐµ ÑÐºÐ´Ð¸ÑÑÐµÑÐ¾Ð½Ð° Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸-Ð°ÐºÑÐ¸Ð²Ð½ÑÑ Ð¿ÑÐ¸Ð¼ÐµÑÐµÐ¹. ÐÑÐ½Ð¾Ð²ÑÐ²Ð°ÑÑÑ Ð½Ð° Ð´Ð°Ð½Ð½ÑÑ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ°Ñ, Ð²Ð¸Ð´Ð½Ð¾, Ð½Ð°ÑÐºÐ¾Ð»ÑÐºÐ¾ Ð²Ð°Ð¶Ð½Ð° Ð¿ÑÐ¾ÑÐµÐ´ÑÑÐ° ÑÑÐ°Ð½Ð´Ð°ÑÑÐ¸Ð·Ð°ÑÐ¸Ð¸ ÑÐ°ÑÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ ÑÑÑÑÑ. Ð¢Ð°ÐºÐ¶Ðµ Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼Ñ Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐ¸Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð´Ð»Ñ Ð¿Ð¾Ð½Ð¸Ð¼Ð°Ð½Ð¸Ñ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾ÑÑÐ¸ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ ÑÐºÐ´Ð¸ÑÑÐµÑÐ¾Ð½Ð° Ð² Ð°Ð´ÑÑÐ²Ð°Ð½ÑÐ½Ð¾Ð¹ ÑÐµÑÐ°Ð¿Ð¸Ð¸. ÐÐ·-Ð·Ð° Ð²ÑÑÐ¾ÐºÐ¾Ð¹ ÑÐ¸ÑÐ¾ÑÐ¾ÐºÑÐ¸ÑÐ½Ð¾ÑÑÑÑ Ð¿Ð¾ Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ñ Ðº Ð½Ð¾ÑÐ¼Ð°Ð»ÑÐ½ÑÐ¼ ÑÐºÐ°Ð½ÑÐ¼ (Ð¼Ð¸Ð¾Ð±Ð»Ð°ÑÑÐ°Ð¼ Ð¸ ÑÐ¸Ð±ÑÐ¾Ð±Ð»Ð°ÑÑÐ°Ð¼) Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ Ð³ÐµÐ½Ð¾ÑÐ¾ÐºÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÑÑÐµÑÑÐ°, Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ðµ ÑÐºÐ´Ð¸ÑÑÐµÑÐ¾Ð½Ð° ÑÐ¿Ð¾ÑÑÑÐ¼ÐµÐ½Ð°Ð¼Ð¸ Ð² Ð²Ð¸Ð´Ðµ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ ÑÐºÑÑÑÐ°ÐºÑÐ¾Ð² Ð¸Ð· ÑÐ¸Ð°Ð½Ð¾ÑÐ¸ÑÐ° Ð²ÑÐ·ÑÐ²Ð°ÐµÑ Ð¾Ð¿Ð°ÑÐµÐ½Ð¸Ðµ Ð¸ Ð½ÑÐ¶Ð´Ð°ÐµÑÑÑ Ð²Ð¾ Ð²ÑÐµÑÑÐ¾ÑÐ¾Ð½Ð½ÐµÐ¼ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¸. ÐÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ðµ ÑÑÑÑÑ ÑÐ°ÑÑÐµÐ½Ð¸Ñ ÐÐµÐ²Ð·ÐµÐ¸ ÑÐ°ÑÑÐ¾Ð»Ð¾Ð²Ð¸Ð´Ð½Ð¾Ð¹ Ð¼Ð¾Ð¶ÐµÑ ÑÐ°ÑÑÐ¼Ð°ÑÑÐ¸Ð²Ð°ÑÑÑÑ ÐºÐ°Ðº Ð±ÐµÐ·Ð¾Ð¿Ð°ÑÐ½ÑÐ¹ Ð°Ð»ÑÑÐµÑÐ½Ð°ÑÐ¸Ð²Ð½ÑÐ¹ ÑÐ¸Ð°Ð½Ð¾ÑÐ¸ÑÑ Ð¸ÑÑÐ¾ÑÐ½Ð¸Ðº ÑÐºÐ´Ð¸ÑÑÐµÑÐ¾Ð½Ð°.
description: This work is devoted to the study of the biological properties of "pure" (â¥97%) ecdysterone, as well as its commercial extract from Cyanosis "XÂ®" (ecdysterone content 18%) in cell models of lung carcinoma. The results show that highly purified ecdysterone and commercial concentrated extract from Cyanotis do not affect the proliferation of adenocarcinoma cells. At the same time, a commercial extract, in contrast to a highly purified one, strongly sensitizes not only tumor but normal cells to a drug doxorubicin, which causes concern when used by humans. At the same time, commercial ecdysterone from Cyanotis did not show cytotoxicity to normal cells under normal conditions. Both highly purified and commercial extracts increased the level of ROS, caused opposite changes in the mitochondrial membrane potential, and had different effects on the autophagy process.Â Most likely, the obtained results are explained by the presence of biologically active impurities in the commercial ecdysterone extract. Based on these results, it canÂ be seen how important the standardization procedure for plant materials is. Further studies are also needed to understand the possibility of using ecdysterone in adjuvant therapy. Due to the high cytotoxicity in relation to normal tissues (myoblasts and fibroblasts) under conditions of genotoxic stress, the use of ecdysterone by athletes in the form of various extracts from cyanotis is of concern and needs to be comprehensively studied. The use of raw materials from the Leuzea cartamoides plant can be considered as a safe alternative source of ecdysterone derived from Cyanotis.