title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ Ð±Ð»Ð¾ÐºÐ°ÑÐ¾ÑÐ° ÐºÐ°Ð»ÑÑÐ¸ÐµÐ²ÑÑ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² l-ÑÐ¸Ð¿Ð° Ð¸ÑÑÐ°Ð´Ð¸Ð¿Ð¸Ð½Ð° Ð½Ð° ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÑÑ Ð¿Ð»Ð°ÑÑÐ¸ÑÐ½Ð¾ÑÑÑ Ð² ÑÑÐµÐ·Ð°Ñ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð° Ð¼ÑÑÐµÐ¹ Ð´Ð¸ÐºÐ¾Ð³Ð¾ ÑÐ¸Ð¿Ð° Ð¸ ÑÑÐ°Ð½ÑÐ³ÐµÐ½Ð½Ð¾Ð¹ Ð»Ð¸Ð½Ð¸Ð¸ YAC128
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Effect of the L-type calcium channels inhibitor isradipine on the synaptic plasticity in hippocampal brain slices of wild type mice and transgenic mice line YAC128
Creators: name: ÐÑÑÐºÐ°ÑÐµÐ²Ð°, Ð¡Ð¾ÑÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: Ð±Ð¾Ð»ÐµÐ·Ð½Ñ Ð¥Ð°Ð½ÑÐ¸Ð½Ð³ÑÐ¾Ð½Ð°
Ð¸ÑÑÐ°Ð´Ð¸Ð¿Ð¸Ð½
Ð´Ð¾Ð»Ð³Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð°Ñ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°ÑÐ¸Ñ
ÐºÐ¾Ð³Ð½Ð¸ÑÐ¸Ð²Ð½ÑÐµ Ð½Ð°ÑÑÑÐµÐ½Ð¸Ñ
Ð½ÐµÐ¹ÑÐ¾Ð½Ð°Ð»ÑÐ½Ð°Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ
Huntington's disease
isradipine
long-term potentiation
cognitive disorders
neural activity

description: Ð Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð±Ð»Ð¾ÐºÐ°ÑÐ¾ÑÐ° ÐºÐ°Ð»ÑÑÐ¸ÐµÐ²ÑÑ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² L-ÑÐ¸Ð¿Ð° Ð¸ÑÑÐ°Ð´Ð¸Ð¿Ð¸Ð½Ð° Ð½Ð° ÑÐ°ÑÑÐ¾ÑÑ Ð²Ð¾Ð·Ð½Ð¸ÐºÐ½Ð¾Ð²ÐµÐ½Ð¸Ñ ÑÐ¿Ð¾Ð½ÑÐ°Ð½Ð½ÑÑ Ð²Ð¾Ð·Ð±ÑÐ¶Ð´Ð°ÑÑÐ¸Ñ Ð¿Ð¾ÑÑÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÐ¾ÐºÐ¾Ð² Ñ Ð¿Ð¸ÑÐ°Ð¼Ð¸Ð´Ð½ÑÑ Ð½ÐµÐ¹ÑÐ¾Ð½Ð¾Ð² Ð² Ð¿ÐµÑÐµÐ¶Ð¸Ð²Ð°ÑÑÐ¸Ñ ÑÑÐµÐ·Ð°Ñ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð° Ñ Ð¼ÑÑÐµÐ¹ Ð´Ð¸ÐºÐ¾Ð³Ð¾ ÑÐ¸Ð¿Ð° (ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½Ð°Ñ Ð³ÑÑÐ¿Ð¿Ð°, WT) Ð¸ Ð¼ÑÑÐµÐ¹ Ñ Ð¼Ð¾Ð´ÐµÐ»ÑÑ Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ Ð¥Ð°Ð½ÑÐ¸Ð½Ð³ÑÐ¾Ð½Ð° (ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½Ð°Ñ Ð³ÑÑÐ¿Ð¿Ð°, YAC128) Ð² Ð²Ð¾Ð·ÑÐ°ÑÑÐµ 4-Ñ Ð¼ÐµÑÑÑÐµÐ². Ð¢Ð°ÐºÐ¶Ðµ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð¾ÑÐµÐ½ÐºÐ° ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¿Ð»Ð°ÑÑÐ¸ÑÐ½Ð¾ÑÑÐ¸ Ð² Ð¿ÐµÑÐµÐ¶Ð¸Ð²Ð°ÑÑÐ¸Ñ ÑÑÐµÐ·Ð°Ñ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð° Ð¼ÑÑÐµÐ¹ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½Ð¾Ð¹ Ð¸ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð³ÑÑÐ¿Ð¿ Ð² Ð²Ð¾Ð·ÑÐ°ÑÑÐµ 4 Ð¸ 6 Ð¼ÐµÑÑÑÐµÐ² Ð´Ð¾ Ð¸ Ð¿Ð¾ÑÐ»Ðµ Ð²Ð¾Ð·Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ð¸ÑÑÐ°Ð´Ð¸Ð¿Ð¸Ð½Ð°. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ Ð´Ð°Ð½Ð½ÑÐµ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð»Ð¸, ÑÑÐ¾ Ð¿Ð¾ÑÐ»Ðµ Ð°Ð¿Ð¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð¸ Ð¸ÑÑÐ°Ð´Ð¸Ð¿Ð¸Ð½Ð° Ð½Ð°Ð±Ð»ÑÐ´Ð°ÐµÑÑÑ ÑÐµÐ½Ð´ÐµÐ½ÑÐ¸Ñ Ðº ÑÐ¼ÐµÐ½ÑÑÐµÐ½Ð¸Ñ ÑÐ°ÑÑÐ¾ÑÑ Ð²Ð¾Ð·Ð½Ð¸ÐºÐ½Ð¾Ð²ÐµÐ½Ð¸Ñ ÑÐ¿Ð¾Ð½ÑÐ°Ð½Ð½ÑÑ Ð²Ð¾Ð·Ð±ÑÐ¶Ð´Ð°ÑÑÐ¸Ñ Ð¿Ð¾ÑÑÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÐ¾ÐºÐ¾Ð² Ñ Ð¼ÑÑÐµÐ¹ Ð»Ð¸Ð½Ð¸Ð¸ YAC128 Ð¸ Ð¿ÑÐ¸ ÑÑÐ¾Ð¼ Ð¸ÑÑÐ°Ð´Ð¸Ð¿Ð¸Ð½ Ð´Ð¾ÑÑÐ¾Ð²ÐµÑÐ½Ð¾ Ð½Ðµ Ð²Ð»Ð¸ÑÐµÑ Ð½Ð° ÑÐ°ÑÑÐ¾ÑÑ Ð¿Ð¾ÑÑÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÐ¾ÐºÐ¾Ð² Ñ Ð¼ÑÑÐµÐ¹ Ð´Ð¸ÐºÐ¾Ð³Ð¾ ÑÐ¸Ð¿Ð°. ÐÑÐµÐ½ÐºÐ° ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¿Ð»Ð°ÑÑÐ¸ÑÐ½Ð¾ÑÑÐ¸ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð»Ð° Ð´Ð¾ÑÑÐ¾Ð²ÐµÑÐ½Ð¾Ðµ Ð¾ÑÐ»Ð°Ð±Ð»ÐµÐ½Ð¸Ðµ Ð´Ð¾Ð»Ð³Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð¹ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°ÑÐ¸Ð¸ Ñ Ð¼ÑÑÐµÐ¹ Ñ Ð¼Ð¾Ð´ÐµÐ»ÑÑ Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ Ð¥Ð°Ð½ÑÐ¸Ð½Ð³ÑÐ¾Ð½Ð° Ð¿Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ Ñ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½Ð¾Ð¹ Ð³ÑÑÐ¿Ð¿Ð¾Ð¹ Ð² Ð²Ð¾Ð·ÑÐ°ÑÑÐµ 4 Ð¸ 6 Ð¼ÐµÑÑÑÐµÐ². Ð ÑÐ»ÑÑÐ°Ðµ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½Ð¾Ð¹ Ð³ÑÑÐ¿Ð¿Ñ Ð¼ÑÑÐµÐ¹ Ð² Ð²Ð¾Ð·ÑÐ°ÑÑÐµ 4-Ñ Ð¼ÐµÑÑÑÐµÐ² Ð°Ð¿Ð¿Ð»Ð¸ÐºÐ°ÑÐ¸Ñ Ð¸ÑÑÐ°Ð´Ð¸Ð¿Ð¸Ð½Ð° Ð¿ÑÐ¸Ð²Ð¾Ð´Ð¸Ð»Ð° Ðº Ð´Ð¾ÑÑÐ¾Ð²ÐµÑÐ½Ð¾Ð¼Ñ Ð¾ÑÐ»Ð°Ð±Ð»ÐµÐ½Ð¸Ñ Ð´Ð¾Ð»Ð³Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð¹ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°ÑÐ¸Ð¸, Ð° Ð² ÑÐ»ÑÑÐ°Ðµ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð³ÑÑÐ¿Ð¿Ñ Ð¼ÑÑÐµÐ¹ Ð°Ð½Ð°Ð»Ð¾Ð³Ð¸ÑÐ½Ð¾Ð³Ð¾ Ð²Ð¾Ð·ÑÐ°ÑÑÐ° Ð½Ð°Ð±Ð»ÑÐ´Ð°Ð»ÑÑ ÑÑÐµÐ½Ð´ Ðº ÑÐ»ÑÑÑÐµÐ½Ð¸Ñ Ð² ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ Ð´Ð¾Ð»Ð³Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð¹ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°ÑÐ¸Ð¸. Ð ÑÐ¾ Ð¶Ðµ Ð²ÑÐµÐ¼Ñ Ñ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½Ð¾Ð¹ Ð¸ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð³ÑÑÐ¿Ð¿ Ð¼ÑÑÐµÐ¹ Ð² Ð²Ð¾Ð·ÑÐ°ÑÑÐµ 6-ÑÐ¸ Ð¼ÐµÑÑÑÐµÐ² Ð¿Ð¾ÑÐ»Ðµ Ð°Ð¿Ð¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð¸ Ð¸ÑÑÐ°Ð´Ð¸Ð¿Ð¸Ð½Ð° Ð´Ð¾Ð»Ð³Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð°Ñ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°ÑÐ¸Ñ Ð½Ðµ ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð»Ð°ÑÑ. Ð¢Ð°ÐºÐ¸Ð¼ Ð¾Ð±ÑÐ°Ð·Ð¾Ð¼, Ð¼Ð¾Ð¶Ð½Ð¾ ÑÐ´ÐµÐ»Ð°ÑÑ Ð²ÑÐ²Ð¾Ð´ Ð¾ ÑÐ¾Ð¼, ÑÑÐ¾ Ñ Ð¼ÑÑÐµÐ¹ Ð´Ð¸ÐºÐ¾Ð³Ð¾ ÑÐ¸Ð¿Ð° Ð¸ Ð¼ÑÑÐµÐ¹ Ñ Ð¼Ð¾Ð´ÐµÐ»ÑÑ Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ Ð¥Ð°Ð½ÑÐ¸Ð½Ð³ÑÐ¾Ð½Ð° Ð½Ð°Ð±Ð»ÑÐ´Ð°ÑÑÑÑ ÑÐ°Ð·Ð»Ð¸ÑÐ¸Ñ Ð² ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¿Ð»Ð°ÑÑÐ¸ÑÐ½Ð¾ÑÑÐ¸. ÐÑÑÐ¾Ð´Ñ Ð¸Ð· Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð´Ð°Ð½Ð½ÑÑ, Ð¸ÑÑÐ°Ð´Ð¸Ð¿Ð¸Ð½ ÑÐ»ÑÑÑÐ°ÐµÑ ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð´Ð¾Ð»Ð³Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð¹ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°ÑÐ¸Ð¸ ÑÐ¾Ð»ÑÐºÐ¾ Ñ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð³ÑÑÐ¿Ð¿Ñ Ð¼ÑÑÐµÐ¹ Ð² Ð²Ð¾Ð·ÑÐ°ÑÑÐµ 4-Ñ Ð¼ÐµÑÑÑÐµÐ². ÐÐ¾ Ð²ÑÐµÑ Ð¾ÑÑÐ°Ð»ÑÐ½ÑÑ ÑÐ»ÑÑÐ°ÑÑ Ð¸ÑÑÐ°Ð´Ð¸Ð¿Ð¸Ð½ Ð¿ÑÐ¾ÑÐ²Ð»ÑÐµÑ ÑÐ¾ÐºÑÐ¸ÑÐµÑÐºÐ¸Ð¹ ÑÑÑÐµÐºÑ, Ð±Ð»Ð¾ÐºÐ¸ÑÑÑ ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð´Ð¾Ð»Ð³Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð¹ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°ÑÐ¸Ð¸.
description: In the present study, we analysed the effect of the L-type calcium channel blocker isradipine on the frequency of spontaneous excitatory postsynaptic currents in pyramidal neurons in surviving hippocampal slices in wild-type mice (control group, WT) and Huntington's disease mice (experimental group, YAC128) aged 4 months. We also evaluated synaptic plasticity in the surviving hippocampal slices of mice in the control and experimental groups at the age of 4 and 6 months before and after isradipine application. The obtained electrophysiological data indicates that after the application of isradipine, there is a tendency to a decrease in the frequency of occurrence of spontaneous excitatory postsynaptic currents in YAC128 mice, while isradipine does not significantly affect the frequency of postsynaptic currents in wild-type mice. Evaluation of synaptic plasticity showed a significant impairment of long-term potentiation in Huntington's disease mice compared with the control group at the age of 4 and 6 months. In the case of the control group of mice at the age of 4 months, the application of isradipine led to a significant weakening of long-term potentiation, and in the case of the experimental group of mice of the same age, a trend towards improvement in the formation of long-term potentiation was observed. At the same time, in the control and experimental groups of mice at the age of 6 months after the application of isradipine, long-term potentiation did not form. Thus, it can be concluded that difference in synaptic plasticity is observed in wild-type and Huntington's disease mice. Based on the obtained data, isradipine improves the formation of long-term potentiation only in the experimental group of mice at the age of 4 months. In all other cases, isradipine demonstrates a toxic effect, blocking the formation of long-term potentiation.