Additional file 1 of PITPNC1 Suppress CD8+ T cell immune function and promote radioresistance in rectal cancer by modulating FASN/CD155
Creators: name: Liang, Junxian
name: Liao, Limin
name: Xie, Lang
name: Tang, WenWen
name: Yu, Xiang
name: Lu, Yinghao
name: Chen, Hongzhen
name: Xu, Juanli
name: Sun, Lei
name: Wu, Huanmei
name: Cui, Chunhui
name: Tan, Yujing
Publisher: name: figshare
Year: 2024
Subjects: subject: Immunology
subject: FOS: Clinical medicine

description: Additional file 1: Figure S1. A The radiographic characteristics pre- and post-radiotherapy in sensitive and unsensitive patients, specifically focusing on the tumor shrinkage percentage. N=7. B The analysis of the correlation between PITPNC1 and CD8+ T cell. Efficiency verification of PITPNC1 (C) and FASN (D) knocking down or FASN overexpression (E) in HT29-RR and SW480-RR cell lines or MC38-RR cell line (F). N=3. Data indicate the mean ± SD. *p &lt; 0.05, **p &lt; 0.01, and ***p &lt; 0.001, by 2-tailed Student’s t test or one-way ANOVA. Figure S2. A Flow cytometry was performed to analyze the changes in the proportion of CD8+ T cells after co-culturing PBMCs with tumor cells. B The alterations in the proportion of IFN-γ+ CD8+ T cells assessed by flow cytometry after co-culturing PBMCs with tumor cells. C–D Analysis of the proportion of DCs in the co-culture through flow cytometry. E The flow cytometry analysis of the apoptosis rate of tumor cells after co-culture. Data indicate the mean ± SD. *p &lt; 0.05, **p &lt; 0.01, and ***p &lt; 0.001, by 2-tailed Student’s t test or one-way ANOVA. N=3. Figure S3. A Flow cytometry analysis of the proportion of CD8+ T cells in the tumor tissues. Proportion of CD4+ T cells, CD8+ T cells (B) and DCs (C) in the blood of mice analyzed by flow cytometry. Flow cytometry analysis of the proportion of CD4+ T cells (D) and DCs (E) in the tumor tissues from mice. F Immunohistochemical analysis of protein expression levels of CD11c in mouse tissues. G, H Immunofluorescence analysis of CD4+ T cells and DCs in mouse tumor tissues. Data indicate the mean ± SD. *p &lt; 0.05, **p &lt; 0.01, and ***p &lt; 0.001, by 2-tailed Student’s t test or one-way ANOVA. N=5. Figure S4. Immunofluorescence co-localization of PITPNC1 and FASN proteins in cells. Data indicate the mean ± SD. *p &lt; 0.05, **p &lt; 0.01, and ***p &lt; 0.001, by 2-tailed Student’s t test or one-way ANOVA. N=3. Figure S5. A The analysis of the proportion of CD8+ T cells after co-culturing PBMCs with tumor cells performed by flow cytometry. B After co-culturing PBMCs with tumor cells, the flow cytometry analysis demonstrated the changes in the proportion of IFN-γ+ CD8+ T cells. C Analysis of the alterations in the proportion of DCs exhibited by flow cytometry after co-culturing PBMCs with the tumor cells. D The flow cytometry analysis of the apoptosis rate of tumor cells in co-cultivation of PBMCs and tumor cells. E, F After co-culturing CD8+ T cells with tumor cells, the flow cytometry analysis demonstrated the changes in the proportion of IFN-γ+ CD8+ T cells. G, H The flow cytometry analysis of the apoptosis rate of tumor cells in co-cultivation of CD8+ T cells and tumor cells. Data indicate the mean ± SD. *p &lt; 0.05, **p &lt; 0.01, and ***p &lt; 0.001, by 2-tailed Student’s t test or one-way ANOVA. N=3. Figure S6. A Analysis of the proportion of CD8+ T cells in the tumor tissues using flow cytometry. Analysis of murine blood CD4+ T cells, CD8+ T cells (B) and DCs (C) proportions using flow cytometry. D, E Flow cytometry analysis of CD4+ T cells and DCs proportions in murine tumor tissues. Data indicate the mean ± SD. *p &lt; 0.05, **p &lt; 0.01, and ***p &lt; 0.001, by 2-tailed Student’s t test or one-way ANOVA. N=5. Figure S7. A Immunohistochemical analysis of protein expression levels of CD11c in mouse tumor tissues. B, C Immunofluorescence analysis of CD4+ T cells and DCs in mouse tumor tissues. Data indicate the mean ± SD. *p &lt; 0.05, **p &lt; 0.01, and ***p &lt; 0.001, by 2-tailed Student’s t test or one-way ANOVA. N=5.