title: ÐÐµÐºÐ°Ð½Ð¾Ð½Ð¸ÑÐµÑÐºÐ°Ñ ÑÐ¾Ð»Ñ ÑÐ»ÐµÐºÑÑÐ¾Ð²Ð¾Ð·Ð±ÑÐ´Ð¸Ð¼ÑÑ Ð½Ð°ÑÑÐ¸ÐµÐ²ÑÑ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² Ð² ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ð°Ð»ÑÐ½ÑÑ Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Noncanonical role of voltage-gated sodium channels in human endometrial mesenchymal stem cells
Creators: name: Ð¨Ð°Ð¼Ð°ÑÐ¾Ð²Ð°, ÐÐ°ÑÐ³Ð°ÑÐ¸ÑÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÐÐ»ÐµÑÐºÐ° (Ð±Ð¸Ð¾Ð».)
ÐÐ¸Ð¾ÑÐµÑ Ð½Ð¾Ð»Ð¾Ð³Ð¸Ñ
ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ð°Ð»ÑÐ½ÑÐµ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°
ÑÐ»ÐµÐºÑÑÐ¾Ð²Ð¾Ð·Ð±ÑÐ´Ð¸Ð¼ÑÐµ Ð½Ð°ÑÑÐ¸ÐµÐ²ÑÐµ ÐºÐ°Ð½Ð°Ð»Ñ
Ð¼Ð¸Ð³ÑÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ð°Ð»ÑÐ½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°
human endometrial mesenchymal stem cells
voltage-gated sodium channels
migration ability of human endometrial stem cells

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«ÐÐµÐºÐ°Ð½Ð¾Ð½Ð¸ÑÐµÑÐºÐ°Ñ ÑÐ¾Ð»Ñ ÑÐ»ÐµÐºÑÑÐ¾Ð²Ð¾Ð·Ð±ÑÐ´Ð¸Ð¼ÑÑ Ð½Ð°ÑÑÐ¸ÐµÐ²ÑÑ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² Ð² ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ð°Ð»ÑÐ½ÑÑ Ð¼ÐµÐ·ÐµÐ½ÑÐ¸Ð¼Ð½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°Â». Ð Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð»Ð°ÑÑ ÑÐµÐ³ÑÐ»ÑÑÐ¸Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÑÐ»ÐµÐºÑÑÐ¾Ð²Ð¾Ð·Ð±ÑÐ´Ð¸Ð¼ÑÑ Ð½Ð°ÑÑÐ¸ÐµÐ²ÑÑ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² (Nav) Ð² ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ð°Ð»ÑÐ½ÑÑ Ð¼ÐµÐ·ÐµÐ½ÑÐ¸Ð¼Ð½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° (ÑÐÐ¡Ð), Ð° ÑÐ°ÐºÐ¶Ðµ ÑÐ¾Ð»Ñ ÑÑÐ¸Ñ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² Ð² Ð¿ÑÐ¾ÑÐµÑÑÐ°Ñ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ Ð¿Ð¾Ð´Ð²Ð¸Ð¶Ð½Ð¾ÑÑÐ¸. Ð¡ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° ÐÐ¢-ÐÐ¦Ð  Ð±ÑÐ»Ð° Ð²ÑÑÐ²Ð»ÐµÐ½Ð° ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Nav 1.1, Nav 1.2, Nav 1.4 -1.9 Ð² ÑÐÐ¡Ð. Ð ÑÐ°ÐºÐ¶Ðµ Ð±ÑÐ»Ð° Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð° ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ã1, Ã3 Ð¸ Ã4 ÑÑÐ±ÑÐµÐ´Ð¸Ð½Ð¸Ñ. ÐÑÐ¿Ð¾Ð»ÑÐ·ÑÑ Ð¼ÐµÑÐ¾Ð´ Ð¿Ð°ÑÑ-ÐºÐ»Ð°Ð¼Ð¿ Ð² ÑÐµÐ¶Ð¸Ð¼Ðµ ÑÐµÐ³Ð¸ÑÑÑÐ°ÑÐ¸Ð¸ ÑÐ¾ÐºÐ¾Ð² whole-cell Ð±ÑÐ»Ð° Ð¸Ð·ÑÑÐµÐ½Ð° ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð°Ð»ÑÐ½Ð°Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÑÐ»ÐµÐºÑÑÐ¾Ð²Ð¾Ð·Ð±ÑÐ´Ð¸Ð¼ÑÑ Ð½Ð°ÑÑÐ¸ÐµÐ²ÑÑ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² Ð² Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ðµ ÐºÐ»ÐµÑÐ¾Ðº ÑÐÐ¡Ð. Ð¡ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾ÑÐ½Ð¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð±ÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ ÐºÐ°Ð½Ð°Ð»Ñ ÑÐ²Ð»ÑÑÑÑÑ Ð¢Ð¢Ð¥- ÑÑÐ²ÑÑÐ²Ð¸ÑÐµÐ»ÑÐ½ÑÐ¼Ð¸. ÐÐ· Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð² Ð¼Ð¾Ð¶Ð½Ð¾ ÑÐ´ÐµÐ»Ð°ÑÑ Ð²ÑÐ²Ð¾Ð´, ÑÑÐ¾ Ð½Ð°Ð±Ð»ÑÐ´Ð°ÐµÐ¼ÑÐµ ÐºÐ°Ð½Ð°Ð»Ñ Ð¼Ð¾Ð³ÑÑ Ð¾ÑÐ½Ð¾ÑÐ¸ÑÑÑÑ Ðº Ð¾Ð´Ð½Ð¾Ð¼Ñ Ð¸Ð· ÑÐ»ÐµÐ´ÑÑÑÐ¸Ñ Ð²Ð°ÑÐ¸Ð°Ð½ÑÐ¾Ð²: Nav 1.1, Nav 1.2, Nav 1.3, Nav 1.6, Nav 1.7. Ð¢Ð°Ðº Ð¶Ðµ, Ð¸ÑÐ¿Ð¾Ð»ÑÐ·ÑÑ Ð¼ÐµÑÐ¾Ð´ Ð¿Ð°ÑÑ-ÐºÐ»Ð°Ð¼Ð¿, Ð±ÑÐ»Ð¾ Ð¸Ð·ÑÑÐµÐ½Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð²ÐµÑÐ°ÑÑÐ¸Ð´Ð¸Ð½Ð° Ð½Ð° Ð±Ð¸Ð¾ÑÐ¸Ð·Ð¸ÑÐµÑÐºÐ¸Ðµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° Nav ÑÐÐ¡Ð. ÐÐµÑÐ°ÑÑÐ¸Ð´Ð¸Ð½ Ð² ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ Ð¼ÐµÐ½ÐµÐµ 40 Ð¼ÐºÐ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾Ð²Ð°Ð» Ð½Ð°ÑÑÐ¸ÐµÐ²ÑÐµ ÑÐ¾ÐºÐ¸, Ð° Ð² Ð½ÐµÑÐºÐ¾Ð»ÑÐºÐ¸Ð¼Ð¸ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ñ Ð¿ÑÐ¸ Ð¿Ð¾Ð´Ð°ÑÐµ 100 Ð¼ÐºÐ Ð²ÐµÑÐ°ÑÑÐ¸Ð´Ð¸Ð½Ð° Ð½Ð°Ð±Ð»ÑÐ´Ð°Ð»Ð¸ ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ðµ Ð²ÑÐµÐ¼ÐµÐ½Ð¸ Ð¾ÑÐºÑÑÑÐ¾Ð³Ð¾ ÑÐ¾ÑÑÐ¾ÑÐ½Ð¸Ñ ÐºÐ°Ð½Ð°Ð»Ð¾Ð². ÐÐ¸Ð³ÑÐ°ÑÐ¸Ð¾Ð½Ð½ÑÐµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° ÑÐÐ¡Ð Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð»Ð¸ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð·Ð°Ð¶Ð¸Ð²Ð»ÐµÐ½Ð¸Ñ Â«ÑÐ°Ð½ÑÂ» Ð¸ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð½Ð¾Ð¹ Ð¼Ð¸Ð³ÑÐ°ÑÐ¸Ð¸ ÑÐµÑÐµÐ· Ð¿Ð¾Ð»ÑÐ¿ÑÐ¾Ð½Ð¸ÑÐ°ÐµÐ¼ÑÑ Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ñ. ÐÑÐ»Ð¾ Ð²ÑÑÐ²Ð»ÐµÐ½Ð¾, ÑÑÐ¾ Ð²ÐµÑÐ°ÑÑÐ¸Ð´Ð¸Ð½ ÑÐ¼ÐµÐ½ÑÑÐ°ÐµÑ Ð¼Ð¸Ð³ÑÐ°ÑÐ¸Ð¾Ð½Ð½ÑÑ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ ÑÐÐ¡Ð. Ð¢Ð¢Ð¥ ÑÐ¼ÐµÐ½ÑÑÐ°Ð» ÑÐºÐ¾ÑÐ¾ÑÑÑ Ð·Ð°ÑÐ°ÑÑÐ°Ð½Ð¸Ñ Â«ÑÐ°Ð½ÑÂ», Ð½Ð¾ Ð½Ðµ Ð²Ð»Ð¸ÑÐ» Ð½Ð° Ð¼Ð¸Ð³ÑÐ°ÑÐ¸Ñ ÑÐµÑÐµÐ· Ð¿Ð¾Ð»ÑÐ¿ÑÐ¾Ð½Ð¸ÑÐ°ÐµÐ¼ÑÑ Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ñ.Â ÐÐ·ÑÑÐµÐ½Ð¸Ðµ Nav Ð² ÑÐÐ¡Ð ÑÐ²Ð»ÑÐµÑÑÑ Ð°ÐºÑÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð·Ð°Ð´Ð°ÑÐµÐ¹, ÑÐ°Ðº ÐºÐ°Ðº ÑÐÐ¡Ð Ð¼Ð¾Ð³ÑÑ Ð±ÑÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ Ð² ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐµÑÐ°Ð¿Ð¸Ð¸. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ Ð² Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸ÑÑ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð¿Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÑÑ, ÑÑÐ¾ Ð±Ð»Ð¾ÐºÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Nav ÐºÐ°Ð½Ð°Ð»Ð¾Ð² Ð¼Ð¾Ð¶ÐµÑ Ð¾ÑÐ»Ð°Ð±Ð»ÑÑÑ Ð¼Ð¸Ð³ÑÐ°ÑÐ¸Ð¾Ð½Ð½ÑÑ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ ÑÐÐ¡Ð.
description: Theme: "Noncanonical role of voltage-gated sodium channels in human endometrial mesenchymal stem cells".Â In this work, we studied the regulation of the activity of voltage-gated sodium channels (Nav) in human endometrial mesenchymal stem cells (eMSCs) and the role of these channels in cell motility.Â RT-PCR analysis revealed the expression of Nav 1.1, Nav 1.2, Nav 1.4, Nav 1.5, Nav 1.6, Nav 1.7, Nav 1.8, Nav 1.9 in eMSCs. Also we showed the expression of Ã1, Ã3 and Ã4 subunits. Using the patch-clamp method in the whole-cell configuration, we studied the functional activity of voltage-gated sodium channels in the membrane of eMSC. Inhibitor analysis showed that the channels are TTX-sensitive. From the data obtained, it can be concluded that the observed channels belong to one of the following options: Nav 1.1, Nav 1.2, Nav 1.3, Nav 1.6, Nav 1.7. We studied the effect of veratridine on the biophysical properties of Nav eMSCs using the patch-clamp method. Veratridine at a concentration of less than 40 Î¼M inhibited sodium currents, and applying 100 Î¼M of veratridine, increased the time of the open state of the channels. The migration properties of eMSCs studied by the wound healing assay and by the method of directed migration through a semipermeable membrane. Two methods have shown that veratridine reduces the migratory ability of eMSCs. TTX reduced the wound healing rate, but did not affect migration through the semipermeable membrane.Â The study of Nav in eMSCs is an urgent task, since eMSCs can be used in cell therapy. The results obtained in the studies show that the blocking of Nav channels can decrease the migratory ability of eMSCs.