Additional file 2 of Integrin β3 in forebrain Emx1-expressing cells regulates repetitive self-grooming and sociability in mice
Creators: name: Lopuch, Andrew J.
name: Swinehart, Brian D.
name: Widener, Eden L.
name: Holley, Z. Logan
name: Bland, Katherine M.
name: Handwerk, Christopher J.
name: Brett, Cooper A.
name: Cook, Hollyn N.
name: Kalinowski, Anna R.
name: Rodriguez, Hilda V.
name: Song, M. Irene
name: Vidal, George S.
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Neuroscience
subject: Developmental Biology
subject: 111714 Mental Health
subject: FOS: Health sciences

description: Additional file 2: Table S1. Within-group two-way ANOVA analyses, N, means ± SEM, and three-way ANOVA of self-grooming behaviors. DFn = Degrees of freedom numerator (between-subject degrees of freedom—1); DFd = Degrees of freedom denominator (within-subject degrees of freedom—between-subject degrees of freedom). Table S2. Within-group two-way ANOVA analyses, N, and means ± SEM of EPM, OFT, and MBT behaviors. Table S3. Within-group two-way ANOVA analyses, N, means ± SEM, and three-way ANOVA of sociability behaviors. Table S4. N, means ± SEM and three-way ANOVA of preference for social novelty behaviors. Table S5. Within-group N, means ± SEM of behaviors of WT mice expressing Cre recombinase under the control of Emx1 (Cre +) and WT mice without Cre recombinase expression (Cre−). Table S6. Measurements of brain morphology of WT, cHET, and cKO experimental mice following dissection (N, mean ± SEM). Two-way ANOVA (factors: genotype, sex) of each measurement had p &gt; 0.05, except that sex was a significant factor in cortex length (p = 0.0225) and anteromedial-to-posterolateral length in both hemispheres (left p = 0.0397, right p = 0.0231). Methods: In accordance with the James Madison University Institutional Animal Care and Use Committee, and using guidance from the American Veterinary Medical Association Guidelines for the Euthanasia of Animals, mice were fully anesthetized and unconscious following a lethal intraperitoneal injection of ketamine (240 mg/kg)-xylazine (48 mg/kg). Acepromazine (1.85 mg/kg) was also administered with the ketamine-xylazine as a tranquilizer. Once mice were fully anesthetized and unconscious, they were euthanized by transcardial perfusion with ice-cold 1 × phosphate-buffered saline followed by 4% paraformaldehyde in phosphate-buffered saline. The brains were then post-fixed in 4% paraformaldehyde in 1 × phosphate-buffered saline until dissected. Measurements of brain morphology were done using a Leica macroscope and FIJI software. Widths were determined by the maximum mediolateral measurement. Lengths were determined by the maximum anteroposterior measurement. “AM-PL Length” refers to the length from the most anteromedial (“AM”) point of the cortex to the most posterolateral (“PL”) point of the cortex; separate measurements were taken for the left hemisphere (“L. Hemi.”) and right hemisphere (“R. Hemi.”). Table S7. As in Table S3, except that sociability was measured by time in the chamber with the object (Obj) or stranger 1 (S1), rather than time spent near Obj or S1. Table S8. As in Table S4, except that preference for social novelty was measured by time in the chamber with stranger 1 (S1) or stranger 2 (S2), rather than Obj or S1. Table S9. As in Table 3 and Table S3, except that stranger 1 (S1) was the opposite sex of the experimental mouse. Table S10. As in Table 4 and Table S4, except that stranger 1 (S1) and stranger 2 (S2) were the opposite sex of the experimental mouse.