title: ÐÐ¸Ð¾ÑÐµÑ Ð½Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ð¹ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð» Ð³ÑÐ¸Ð±Ð° Camarosporomyces flavigenus 32.43 Ð´Ð»Ñ Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸ Ð°ÐºÑÐ¸Ð²Ð½ÑÑ Ð²ÑÐ¾ÑÐ¸ÑÐ½ÑÑ Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸ÑÐ¾Ð²
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Biotechnological potential of the fungus Camarosporomyces flavigenus 32.43 for the formation of biologically active secondary metabolites
Creators: name: ÐÑÐ²Ð¾Ð²Ð°, ÐÑÐµÐ½Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Ð²ÑÐ¾ÑÐ¸ÑÐ½ÑÐµ Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸ÑÑ
C. flavigenus 32.43
ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½ÑÐµ ÑÑÐµÐ´Ñ
secondary metabolites
cultivation
nutrient media

description: ÐÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° ÑÐ¾ÑÑÐ¾Ð¸Ñ Ð¸Ð· ÑÑÐµÑ Ð³Ð»Ð°Ð². ÐÐµÑÐ²Ð°Ñ Ð³Ð»Ð°Ð²Ð° Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÑÐµÑ ÑÐ¾Ð±Ð¾Ð¹ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½ÑÐ¹ Ð¾Ð±Ð·Ð¾Ñ, Ð² ÐºÐ¾ÑÐ¾ÑÐ¾Ð¼ ÑÐ°ÑÑÐ¼Ð¾ÑÑÐµÐ½Ñ Ð¿ÐµÑÑÐ¿ÐµÐºÑÐ¸Ð²Ñ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð¿ÑÐ¸ÑÐ¾Ð´Ð½ÑÑ Ð²ÐµÑÐµÑÑÐ² Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð³ÐµÑÐ±Ð¸ÑÐ¸Ð´Ð¾Ð², ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÐµ ÑÐ¿Ð¾ÑÐ¾Ð±Ñ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ ÑÐ¾ÑÑÐ°Ð²Ð° Ð²ÑÐ¾ÑÐ¸ÑÐ½ÑÑ Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸ÑÐ¾Ð² Ð³ÑÐ¸Ð±Ð¾Ð², Ð° ÑÐ°ÐºÐ¶Ðµ ÑÐ°Ð·Ð½Ð¾Ð¾Ð±ÑÐ°Ð·Ð½Ð°Ñ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ°Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð¿ÑÐ¸ÑÐ¾Ð´Ð½ÑÑ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´Ð½ÑÑ Ð¼ÐµÐ»Ð»ÐµÐ¸Ð½Ð¾Ð². ÐÐ¾ Ð²ÑÐ¾ÑÐ¾Ð¹ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½Ð¾Ð¹ ÑÐ°ÑÑÐ¸, Ð¾Ð¿Ð¸ÑÐ°Ð½Ñ ÑÐ¿Ð¾ÑÐ¾Ð±Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð³ÑÐ¸Ð±Ð°, Ð¼ÐµÑÐ¾Ð´Ð¸ÐºÐ¸ ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸ Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸ÑÐ½ÑÑ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ¾Ð², ÑÐ¿Ð¾ÑÐ¾Ð±Ð¾Ð² ÑÑÐ°ÐºÑÐ¸Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ, Ð¾ÑÐµÐ½ÐºÐ¸ Ð¸Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ ÑÐ¿ÐµÐºÑÑÐ°Ð»ÑÐ½ÑÑ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸Ðº Ð¸Ð½Ð´Ð¸Ð²Ð¸Ð´ÑÐ°Ð»ÑÐ½ÑÑ ÑÐ¾ÐµÐ´Ð¸Ð½ÐµÐ½Ð¸Ð¹. Ð Ð°ÑÑÐ¼Ð¾ÑÑÐµÐ½Ñ Ð¼ÐµÑÐ¾Ð´Ð¸ÐºÐ¸ Ð¾ÑÐµÐ½ÐºÐ¸ Ð³ÐµÑÐ±Ð¸ÑÐ¸Ð´Ð½Ð¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÑÐ¸ÑÐ¾ÑÐ¾ÐºÑÐ¸Ð½Ð¾Ð² Ð³ÑÐ¸Ð±Ð° Ð½Ð° Ð»Ð¸ÑÑÐ¾Ð²ÑÑ Ð´Ð¸ÑÐºÐ°Ñ, Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð¸Ð½ÑÑÐµÐºÑÐ¸ÑÐ¸Ð´Ð½Ð¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð½Ð° Galleria melonella, Ð±ÑÐ»Ð° Ð¿ÑÐ¾Ð¸Ð·Ð²ÐµÐ´ÐµÐ½Ð° Ð¾ÑÐµÐ½ÐºÐ° Ð¾Ð±ÑÐµÐ¹ ÑÐ¾ÐºÑÐ¸ÑÐ½Ð¾ÑÑÐ¸ ÑÐºÑÑÑÐ°ÐºÑÐ¾Ð² Ð½Ð° ÐºÑÐ»ÑÑÑÑÐµ Ð¸Ð½ÑÑÐ·Ð¾ÑÐ¸Ð¸-ÑÑÑÐµÐ»ÑÐºÐ¸, Ð° ÑÐ°ÐºÐ¶Ðµ Ð°Ð½ÑÐ¸Ð¼Ð¸ÐºÑÐ¾Ð±Ð½Ð°Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÑÐºÑÑÑÐ°ÐºÑÐ¾Ð² Ð¸ Ð¸Ð½Ð´Ð¸Ð²Ð¸Ð´ÑÐ°Ð»ÑÐ½ÑÑ ÑÐ¾ÐµÐ´Ð¸Ð½ÐµÐ½Ð¸Ð¹ Ð¸ Ð¿ÑÐ¾Ð²ÐµÑÐºÐ° Ð½Ð° ÑÐ¸ÑÐ¾ÑÐ¾ÐºÑÐ¸ÑÐµÑÐºÑÑ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð½Ð° ÐºÐ»ÐµÑÐºÐ°Ñ Sf 9. ÐÐ·ÑÑÐµÐ½Ð¸Ðµ Ð¸Ð½Ð´Ð¸Ð²Ð¸Ð´ÑÐ°Ð»ÑÐ½ÑÑ ÐºÐ¾Ð¼Ð¿Ð¾Ð½ÐµÐ½ÑÐ¾Ð² ÑÐºÑÑÑÐ°ÐºÑÐ¾Ð² C. flavigenus 32.43 Ð¿ÑÐ¾Ð´ÐµÐ¼Ð¾Ð½ÑÑÑÐ¸ÑÐ¾Ð²Ð°Ð»Ð¾, ÑÑÐ¾ ÑÑÐ¾Ñ Ð³ÑÐ¸Ð± ÑÐ¿Ð¾ÑÐ¾Ð±ÐµÐ½ Ð¿ÑÐ¾Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°ÑÑ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸ Ð°ÐºÑÐ¸Ð²Ð½ÑÐµ Ð²ÐµÑÐµÑÑÐ²Ð° Ð¸Ð· ÑÐ°Ð·Ð½ÑÑ Ð³ÑÑÐ¿Ð¿ Ð¿ÑÐ¸ÑÐ¾Ð´Ð½ÑÑ ÑÐ¾ÐµÐ´Ð¸Ð½ÐµÐ½Ð¸Ð¹ â Ð²ÐµÑÐ¼ÐµÐ»ÑÐ¾ÑÐ¸Ð½ (Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´Ð½Ð¾Ðµ ÑÐµÑÑÐ°Ð¼Ð¾Ð²Ð¾Ð¹ ÐºÐ¸ÑÐ»Ð¾ÑÑ), ÐºÐ»Ð°Ð²Ð°ÑÐ¾Ð» (Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´Ð½Ð¾Ðµ ÑÐµÐ½Ð¾Ð»Ð°), 6-Ð³Ð¸Ð´ÑÐ¾ÐºÑÐ¸Ð¼ÐµÐ»Ð»ÐµÐ¸Ð½ Ð¸ 7-ÑÐ»Ð¾Ñ-8-Ð¼ÐµÑÐ¾ÐºÑÐ¸Ð¼ÐµÐ»Ð»ÐµÐ¸Ð½ (Ð¼ÐµÐ»Ð»ÐµÐ¸Ð½Ñ). Ð ÑÑÐµÑÑÐµÐ¹ Ð³Ð»Ð°Ð²Ðµ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÐµÐ½Ñ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸ÑÐ½ÑÑ Ð¿ÑÐ¾ÑÐ¸Ð»ÐµÐ¹ ÑÐºÑÑÑÐ°ÐºÑÐ¾Ð² ÐºÑÐ»ÑÑÑÑ C. flavigenus 32.43 Ð¿ÑÐ¸ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ ÑÐ¿Ð¾ÑÐ¾Ð±Ð°Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¸ Ð¸Ñ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð°; Ð¸ÑÐ¾Ð³Ð¸ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¸ Ð¸Ð´ÐµÐ½ÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Ð¸Ð½Ð´Ð¸Ð²Ð¸Ð´ÑÐ°Ð»ÑÐ½ÑÑ ÑÐ¾ÐµÐ´Ð¸Ð½ÐµÐ½Ð¸Ð¹; Ð¾ÑÐµÐ½ÐºÐ° ÑÐ¿ÐµÐºÑÑÐ° Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸ Ð°ÐºÑÐ¸Ð²Ð½ÑÑ ÑÐ¾ÐµÐ´Ð¸Ð½ÐµÐ½Ð¸Ð¹. ÐÑÐ¸ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð¸Ð¸ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±Ð·Ð¾ÑÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ eLIBRARY.RU, Elsevier ScienceDirect, Scopus, PubMed, Reaxys. Ð¡ÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÑÑ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»Ð¸ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿Ð°ÐºÐµÑÐ° Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼ Microsoft Excel.
description: The final qualifying work consists of three chapters. The first chapter is a literary review, which examines the prospects for the use of natural substances as herbicides, various ways to change the composition of secondary metabolites of fungi, as well as the diverse biological activity of natural derivatives of melleins. In the second experimental part, methods of mushroom cultivation, methods of extraction of metabolic complexes, methods of fractionation, evaluation of their activity, as well as obtaining spectral characteristics of individual compounds are described. The methods of assessing the herbicidal activity of phytotoxins of the fungus on leaf discs, the determination of the insecticidal activity on Galleria melonella, the assessment of the total toxicity of extracts on the culture of the slipper infusoria, as well as the antimicrobial activity of extracts and individual compounds and testing for cytotoxic activity on Sf 9 cells were carried out. The study of individual components of extracts of C. flavigenus 32.43 demonstrated that this fungus is able to produce biologically active substances from different groups of natural compounds â vermelhotin (1) (tetramic acid derivative), clavatol (2) (phenol derivative), 6-hydroxymellein (3) and 7-chloro-8-methoxymellein (4) (melleins). The third chapter presents the results of the analysis of the metabolic profiles of extracts of C. flavigenus 32.43 cultures under various cultivation methods and their biological properties; the results of isolation and identification of individual compounds; evaluation of the spectrum of biological activity of the biologically active compounds obtained. The following eLibrary databases were used when performing the literature review.RU, Elsevier ScienceDirect, Scopus, PubMed, Reaxys. Statistical processing of experimental data was carried out using the Microsoft Excel software package and the website.