title: ÐÑÐµÐ´ÑÐºÐ°Ð·Ð°Ð½Ð¸Ðµ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÐ¸ Ð±ÐµÐ»ÐºÐ° Ðº ÑÐ°Ð·Ð¾Ð²Ð¾Ð¼Ñ ÑÐ°Ð·Ð´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¶Ð¸Ð´ÐºÐ¾ÑÑÑ-Ð¶Ð¸Ð´ÐºÐ¾ÑÑÑ Ð¿Ð¾ ÐµÐ³Ð¾ Ð°Ð¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾ÑÐ½Ð¾Ð¹ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÐ¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Prediction of the ability of a protein to liquid-liquid phase separation by its amino acid sequence
Creators: name: ÐÐ¾ÐºÐ¸Ð½, Ð¯ÐºÐ¾Ð²
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Ð½ÐµÐ¼ÐµÐ¼Ð±ÑÐ°Ð½Ð½ÑÐµ Ð¾ÑÐ³Ð°Ð½ÐµÐ»Ð»Ñ
ÑÐ°Ð·Ð¾Ð²ÑÐ¹ Ð¿ÐµÑÐµÑ Ð¾Ð´ Ð¶Ð¸Ð´ÐºÐ¾ÑÑÑ-Ð¶Ð¸Ð´ÐºÐ¾ÑÑÑ
Ð²Ð½ÑÑÑÐµÐ½Ð½Ðµ Ð½ÐµÑÐ¿Ð¾ÑÑÐ´Ð¾ÑÐµÐ½Ð½ÑÐµ Ð±ÐµÐ»ÐºÐ¸
ÑÐ°ÑÑÐ¾ÑÐ½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð· Ð°Ð¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾Ñ
ÑÐ°Ð½Ð´ÐµÐ¼Ð½ÑÐµ Ð¿Ð¾Ð²ÑÐ¾ÑÑ
membraneless organelle
liquid-liquid phase separation
intrinsically disordered proteins
frequency amino acid analysis
tandem repeat

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«ÐÑÐµÐ´ÑÐºÐ°Ð·Ð°Ð½Ð¸Ðµ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÐ¸ Ð±ÐµÐ»ÐºÐ° Ðº ÑÐ°Ð·Ð¾Ð²Ð¾Ð¼Ñ ÑÐ°Ð·Ð´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¶Ð¸Ð´ÐºÐ¾ÑÑÑ-Ð¶Ð¸Ð´ÐºÐ¾ÑÑÑ Ð¿Ð¾ ÐµÐ³Ð¾ Ð°Ð¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾ÑÐ½Ð¾Ð¹ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÐ¸Â». ÐÐµÐ¼ÐµÐ¼Ð±ÑÐ°Ð½Ð½ÑÐµ Ð¾ÑÐ³Ð°Ð½ÐµÐ»Ð»Ñ Ð¾Ð±ÑÐ°Ð·ÑÑÑÑÑ Ð² ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ ÑÐ°Ð·Ð¾Ð²Ð¾Ð³Ð¾ Ð¿ÐµÑÐµÑÐ¾Ð´Ð° Ð¶Ð¸Ð´ÐºÐ¾ÑÑÑ-Ð¶Ð¸Ð´ÐºÐ¾ÑÑÑ (LLPS) Ð±ÐµÐ»ÐºÐ¾Ð², Ð²ÑÐ¾Ð´ÑÑÐ¸Ñ Ð² ÑÐ¾ÑÑÐ°Ð² Ð´Ð°Ð½Ð½ÑÑ ÐºÐ¾Ð¼Ð¿Ð°ÑÑÐ¼ÐµÐ½ÑÐ¾Ð². Ð¢Ð¾ÑÐ½ÑÐµ Ð¿ÑÐ¸ÑÐ¸Ð½Ñ ÑÑÐ¸Ñ Ð¿ÐµÑÐµÑÐ¾Ð´Ð¾Ð² Ð² ÐºÐ»ÐµÑÐºÐµ Ð´Ð¾ ÑÐ¸Ñ Ð¿Ð¾Ñ Ð½Ðµ ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ñ, Ð½Ð¾ Ð¸Ð·Ð²ÐµÑÑÐ½Ð¾, ÑÑÐ¾ Ð¾Ð½Ð¸ ÑÐ²ÑÐ·Ð½Ñ Ñ Ð½Ð°Ð»Ð¸ÑÐ¸ÐµÐ¼ Ð¼Ð½Ð¾Ð¶ÐµÑÑÐ²ÐµÐ½Ð½ÑÑ Ð¼ÐµÐ¶Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½ÑÑ ÐºÐ¾Ð½ÑÐ°ÐºÑÐ¾Ð² Ð¸ ÑÐ»Ð°Ð±ÑÐ¼Ð¸ Ð½ÐµÐºÐ¾Ð²Ð°Ð»ÐµÐ½ÑÐ½ÑÐ¼Ð¸ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸ÑÐ¼Ð¸ Ð¼ÐµÐ¶Ð´Ñ Ð°Ð¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾ÑÐ°Ð¼Ð¸. ÐÐ½Ð°Ð»Ð¸Ð· Ð°Ð¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾ÑÐ½Ð¾Ð¹ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÐ¸ Ð¼Ð¾Ð¶ÐµÑ Ð¿Ð¾ÐºÐ°Ð·Ð°ÑÑ Ð¾ÑÐ»Ð¸ÑÐ¸Ñ Ð¼ÐµÐ¶Ð´Ñ Ð±ÐµÐ»ÐºÐ°Ð¼Ð¸, ÑÐºÐ»Ð¾Ð½Ð½ÑÐ¼Ð¸ Ðº LLPS Ð¸ Ð¾ÑÑÐ°Ð»ÑÐ½ÑÐ¼ Ð¿ÑÐ¾ÑÐµÐ¾Ð¼Ð¾Ð¼. Ð Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ Ð±ÑÐ»Ð¸ Ð¿ÑÐ¾Ð°Ð½Ð°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°Ð½Ñ Ð´Ð²Ðµ Ð²ÑÐ±Ð¾ÑÐºÐ¸: Ð±ÐµÐ»ÐºÐ¾Ð², ÑÐºÐ»Ð¾Ð½Ð½ÑÑ Ðº LLPS, Ð¸ Ð±ÐµÐ»ÐºÐ¾Ð² Ð¾ÑÑÐ¸ÑÐ°ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ ÐºÐ¾Ð½ÑÑÐ¾Ð»Ñ. ÐÑÐ»Ð¾ Ð²ÑÑÑÐ½ÐµÐ½Ð¾, ÐºÐ°Ðº Ð¾ÑÐ»Ð¸ÑÐ°ÑÑÑÑ ÑÐ°ÑÑÐ¾ÑÑ Ð²ÑÑÑÐµÑÐ°ÐµÐ¼Ð¾ÑÑÐ¸ Ð¾Ð´Ð¸Ð½Ð¾ÑÐ½ÑÑ Ð¸ Ð¿Ð°Ñ Ð°Ð¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾Ñ, ÑÑÐµÐ´Ð½Ð¸Ð¹ Ð·Ð°ÑÑÐ´ Ð¸ ÑÑÐµÐ´Ð½ÑÑ Ð³Ð¸Ð´ÑÐ¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ Ñ Ð±ÐµÐ»ÐºÐ¾Ð² ÑÐºÐ»Ð¾Ð½Ð½ÑÑ Ð¸ Ð½Ðµ ÑÐºÐ»Ð¾Ð½Ð½ÑÑ Ðº LLPS. ÐÑ Ð¿ÑÐ¾Ð²ÐµÑÐ¸Ð»Ð¸ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ ÑÐ°Ð½Ð´ÐµÐ¼Ð½ÑÑ Ð¿Ð¾Ð²ÑÐ¾ÑÐ¾Ð² Ð½Ð° ÑÐºÐ»Ð¾Ð½Ð½Ð¾ÑÑÑ Ð±ÐµÐ»ÐºÐ¾Ð² Ðº LLPS. ÐÐ»Ñ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð±ÑÐ»Ð¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ: ÑÐ°ÑÑÐ¾ÑÐ½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð·, Ð¼ÐµÑÐ¾Ð´ Ð³Ð»Ð°Ð²Ð½ÑÑ ÐºÐ¾Ð¼Ð¿Ð¾Ð½ÐµÐ½Ñ Ð¸ Ð¼ÐµÑÐ¾Ð´ k-Ð±Ð»Ð¸Ð¶Ð°Ð¹ÑÐ¸Ñ ÑÐ¾ÑÐµÐ´ÐµÐ¹. ÐÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð±ÐµÐ»ÐºÐ¸, ÑÐºÐ»Ð¾Ð½Ð½ÑÐµ Ðº LLPS, Ð¼ÐµÐ½ÐµÐµ Ð³Ð¸Ð´ÑÐ¾ÑÐ¾Ð±Ð½Ñ, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¸Ð¼ÐµÑÑ Ð¸Ð·Ð±ÑÑÐ¾Ðº ÑÐ°ÐºÐ¸Ñ Ð°Ð¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾Ñ ÐºÐ°Ðº G, Q, S, P Ð¸ Ð´ÐµÑÐ¸ÑÐ¸Ñ I, L, Ð¡, W. ÐÐµÐ»ÐºÐ¸, ÑÐºÐ»Ð¾Ð½Ð½ÑÐµ Ðº LLPS, Ð¸Ð¼ÐµÑÑ Ð¿Ð¾Ð²ÑÑÐµÐ½Ð½Ð¾Ðµ ÑÐ¸ÑÐ»Ð¾ ÑÐ°Ð½Ð´ÐµÐ¼Ð½ÑÑ Ð¿Ð¾Ð²ÑÐ¾ÑÐ¾Ð², Ð¾Ð±ÑÐ°Ð·ÑÑÑÐ¸Ñ Ð³Ð¸Ð´ÑÐ¾ÑÐ¾Ð±Ð½ÑÐµ ÐºÐ»Ð°ÑÑÐµÑÑ Ð² Ð´Ð¾Ð¼ÐµÐ½Ð°Ñ Ð½Ð¸Ð·ÐºÐ¾Ð¹ ÑÐ»Ð¾Ð¶Ð½Ð¾ÑÑÐ¸, Ð° ÑÐ°ÐºÐ¶Ðµ Ð·Ð°ÑÑÐ¶ÐµÐ½Ð½ÑÑ ÑÑÐ°ÑÑÐºÐ¾Ð², Ð¿ÑÐ¸Ð½Ð¸Ð¼Ð°ÑÑÐ¸Ñ ÑÑÐ°ÑÑÐ¸Ðµ Ð² ÑÐ»ÐµÐºÑÑÐ¾ÑÑÐ°ÑÐ¸ÑÐµÑÐºÐ¾Ð¼ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ð¸. ÐÐ° Ð¾ÑÐ½Ð¾Ð²Ð°Ð½Ð¸Ð¸ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð±ÑÐ» ÑÐ¾Ð·Ð´Ð°Ð½ ÐºÐ»Ð°ÑÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¾Ñ Ð±ÐµÐ»ÐºÐ¾Ð², ÑÐºÐ»Ð¾Ð½Ð½ÑÑ Ðº LLPS, Ð¿Ð¾ Ð¸Ñ Ð°Ð¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾ÑÐ½Ð¾Ð¹ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÐ¸.
description: Membraneless organelles are formed as a result of the liquid-liquid phase separations (LLPS) of the proteins that make up these compartments. The exact reasons for these transitions in the cell have not yet been established, but it is known that they are associated with the presence of multiple intermolecular contacts and weak non-covalent interactions between amino acids. Amino acid sequence analysis can show differences between LLPS proteins and other proteomic proteins. In this work, two samples were analyzed: LLPS proteins and proteins of negative control. It was found out how the frequencies of occurrence of single and pairs of amino acids, the average charge and average hydrophobicity differ in LLPS proteins and non-LLPS proteins. We checked the influence of tandem repeats on the propensity of proteins to LLPS. Frequency analysis, principal component method and k-nearest neighbor method were used for the analysis. We found out that LLPS proteins are less hydrophobic, and also have an excess of amino acids such as G, Q, S, P and a deficiency of I, L, C, W. LLPS proteins have an increased number of tandem repeats that form hydrophobic clusters in domains of low complexity, as well as charged regions involved in electrostatic interaction. Based on the analysis, a classifier of LLPS proteins by their amino acid sequence was created.