title: Ð Ð¾Ð»Ñ Ð²Ð½ÐµÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ Ð¼Ð°ÑÑÐ¸ÐºÑÐ° Ð² ÑÐµÐ³ÑÐ»ÑÑÐ¸Ð¸/Ð¼Ð¾Ð´ÑÐ»ÑÑÐ¸Ð¸/ ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÑ ÑÑÑÐ¾Ð¼Ð°Ð»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Extracellular matrix participation in regulation/modulation/functioning of human mesenchymal stromal cells
Creators: name: ÐÐµÑÐµÐ²Ð¾Ð·Ð½Ð¸ÐºÐ¾Ð², ÐÐ»ÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Ð²Ð½ÐµÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐ¹ Ð¼Ð°ÑÑÐ¸ÐºÑ (ÐÐÐ)
ÑÐÐ¡Ð
ÐÐ¡Ð ÐÐ°ÑÑÐ¾Ð½Ð¾Ð²Ð° ÑÑÑÐ´Ð½Ñ
ÑÑÑÐµÑÑ-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ðµ ÑÑÐ°ÑÐµÐ½Ð¸Ðµ
Ñ Ð¾Ð½Ð´ÑÐ¾Ð³ÐµÐ½Ð½Ð°Ñ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐ°
Ð³Ð¸Ð¿Ð¾ÐºÑÐ¸Ñ
Ð¼Ð°ÐºÑÐ¾Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½ÑÐµ ÐºÑÐ°ÑÐ´ÐµÑÑ
extracellular matrix (ECM)
eMSCs
Whartons jelly MSCs
stress-induced senescence
chondrogenic differentiation
hypoxia
macromolecular crowding

description: ÐÑÐµÐ´ÑÑÐ°Ð²Ð»ÐµÐ½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ ÑÐ¾Ð»Ð¸ ÐÐÐ ÑÐ°Ð·Ð»Ð¸ÑÐ½Ð¾Ð¹ Ð¿ÑÐ¸ÑÐ¾Ð´Ñ Ð² ÑÐµÐ³ÑÐ»ÑÑÐ¸Ð¸ ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÑÐÐ¡Ð, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¿Ð¾Ð¸ÑÐºÑ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾ÑÑÐµÐ¹ Ð¾Ð¿ÑÐ¸Ð¼Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð´ÐµÐ¿Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¼Ð°ÑÑÐ¸ÐºÑÐ° ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ ÐºÑÐ»ÑÑÑÑÐ¾Ð¹. ÐÑÐ¸ Ð¾ÑÐµÐ½ÐºÐµ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð´ÐµÐ¿Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÐÐÐ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ Ð¸Ð¼Ð¼ÑÐ½Ð¾ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ðµ Ð¾ÐºÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ðµ Ð¼Ð°ÑÑÐ¸ÐºÑÐ° Ð½Ð° ÑÐ¸Ð±ÑÐ¾Ð½ÐµÐºÑÐ¸Ð½, ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½ I, III Ð¸ IV ÑÐ¸Ð¿Ð¾Ð². ÐÐ»Ñ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° H2O2-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ, ÑÐÐ¡Ð Ð¾ÐºÑÐ°ÑÐ¸Ð²Ð°Ð»Ð¸ Ð´Ð»Ñ Ð¿ÑÐ¾Ð²ÐµÑÐºÐ¸ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ SA-Î²-Gal, Ð¸Ð·Ð¼ÐµÑÑÐ»Ð¸ Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐ¹ ÑÑÐ¾Ð²ÐµÐ½Ñ ÐÐ¤Ð Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð³Ð¾ Ð·Ð¾Ð½Ð´Ð° H2DCF-DA, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¿ÑÐ¾Ð²ÐµÑÑÐ»Ð¸ ÑÑÐ¾Ð²ÐµÐ½Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ p21, GAPDH Ð¸ ÑÑÐ¾Ð²ÐµÐ½Ñ ÑÐ¾ÑÑÐ¾ÑÐ¸Ð»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Rb Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð²ÐµÑÑÐµÑÐ½-Ð±Ð»Ð¾ÑÑÐ¸Ð½Ð³Ð°. ÐÑÐ¸ Ð¸Ð·ÑÑÐµÐ½Ð¸Ð¸ Ð¿ÑÐ¾ÑÐµÑÑÐ° ÑÐ¾Ð½Ð´ÑÐ¾Ð³ÐµÐ½Ð½Ð¾Ð¹ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐ¸ ÑÐÐ¡Ð Ð¼Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ Ð¾ÐºÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ðµ Ð°Ð»ÑÑÐ¸Ð°Ð½Ð¾Ð²ÑÐ¼ ÑÐ¸Ð½Ð¸Ð¼, Ð¸Ð¼Ð¼ÑÐ½Ð¾ÑÐµÐ½Ð¾ÑÐ¸Ð¿Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ, Ð° ÑÐ°ÐºÐ¶Ðµ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð ÐÐ Ñ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐ¸Ð¼ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸ÐµÐ¼ ÐÐ¢-ÐÐ¦Ð . ÐÐ° Ð¾ÑÐ½Ð¾Ð²Ðµ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð² Ð¼Ñ ÑÑÑÐ°Ð½Ð¾Ð²Ð¸Ð»Ð¸, ÑÑÐ¾ ÑÐ¸ÐºÐ¾Ð»Ð» 400 Ð² ÑÐ¾Ð»Ð¸ ÐºÑÐ°ÑÐ´ÐµÑÐ° Ð¸ Ð³Ð¸Ð¿Ð¾ÐºÑÐ¸ÑÐµÑÐºÐ¸Ðµ ÑÑÐ»Ð¾Ð²Ð¸Ñ ÑÑÐ¸Ð»Ð¸Ð²Ð°ÑÑ Ð¾ÑÐ»Ð¾Ð¶ÐµÐ½Ð¸Ðµ ÐÐÐ ÐºÐ°Ðº Ð²Ð¼ÐµÑÑÐµ, ÑÐ°Ðº Ð¸ Ð¿Ð¾ Ð¾ÑÐ´ÐµÐ»ÑÐ½Ð¾ÑÑÐ¸. ÐÐÐ Ð¾Ñ Ð¼Ð¾Ð»Ð¾Ð´ÑÑ ÐÐ¡Ð WJ-1 ÑÐ¾ÑÐ¼Ð¾Ð·Ð¸Ñ H2O2-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ðµ ÑÑÐ°ÑÐµÐ½Ð¸Ðµ Ð¿Ð¾ÑÐµÑÐ½Ð½ÑÑ Ð½Ð° Ð½ÐµÐ³Ð¾ ÐºÐ»ÐµÑÐ¾Ðº. Ð ÐÐÐ, Ð½Ð°ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð½ÑÐ¹ IGFBP3-Ð½Ð¾ÐºÐ°ÑÑÐ½ÑÐ¼Ð¸ ÑÐÐ¡Ð ÑÑÐºÐ¾ÑÑÐµÑ Ð¿ÑÐ¾ÑÐµÑÑ Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð¹ ÑÐ¾Ð½Ð´ÑÐ¾Ð³ÐµÐ½Ð½Ð¾Ð¹ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐ¸ ÑÐÐ¡Ð. ÐÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð·Ð°ÑÑÐ°Ð³Ð¸Ð²Ð°ÐµÑ Ð°ÐºÑÑÐ°Ð»ÑÐ½ÑÐµ Ð²Ð¾Ð¿ÑÐ¾ÑÑ Ð² ÑÑÐµÑÐµ Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ð¸ ÐÐÐ. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ Ð´Ð°Ð½Ð½ÑÐµ Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÑÑ ÑÐ°ÑÑÐ¸ÑÐ¸ÑÑ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÐµÐ½Ð¸Ñ Ð¾ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ð¸ Â«Ð¼Ð°ÑÑÐ¸ÐºÑ-ÐºÐ»ÐµÑÐºÐ°Â» Ð¸ ÑÐ¿Ð¾ÑÐ¾Ð±Ð°Ñ Ð¿Ð¾Ð²ÑÑÐµÐ½Ð¸Ñ Ð¿ÑÐ¾Ð´ÑÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð½Ð°ÑÐ°Ð±Ð¾ÑÐºÐ¸ ÐÐÐ ÑÐÐ¡Ð. Ð¡ÑÐ¼Ð¼Ð¸ÑÑÑ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÐµ Ð½Ð°Ð¼Ð¸ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ, Ð¼Ð¾Ð¶Ð½Ð¾ ÑÐ´ÐµÐ»Ð°ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð²ÑÐ²Ð¾Ð´Ñ: 1. ÐÐ¸Ð¿Ð¾ÐºÑÐ¸Ñ Ð¸ Ð¼Ð°ÐºÑÐ¾Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½ÑÐ¹ ÐºÑÐ°ÑÐ´ÐµÑ ÑÐ¸ÐºÐ¾Ð»Ð» 400 Ð¿Ð¾Ð²ÑÑÐ°ÑÑ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð´ÐµÐ¿Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÐÐÐ ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ð°Ð»ÑÐ½ÑÐ¼Ð¸ ÐÐ¡Ð ÐºÐ°Ðº Ð¿Ð¾ Ð¾ÑÐ´ÐµÐ»ÑÐ½Ð¾ÑÑÐ¸, ÑÐ°Ðº Ð¸ Ð¿ÑÐ¸ Ð¸Ñ ÑÐ¾Ð²Ð¼ÐµÑÑÐ½Ð¾Ð¼ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¸. 2. ÐÐ¿ÐµÑÐ²ÑÐµ Ð¿ÑÐ¾Ð´ÐµÐ¼Ð¾Ð½ÑÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð° ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ ÐÐÐ Ð¾Ñ Ð¼Ð¾Ð»Ð¾Ð´ÑÑ ÐÐ¡Ð WJ-1 ÑÐ°ÑÑÐ¸ÑÐ½Ð¾ Ð¾Ð±ÑÐ°ÑÐ°ÑÑ/ÑÐ¾ÑÐ¼Ð¾Ð·Ð¸ÑÑ ÑÑÑÐµÑÑ-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ðµ ÑÑÐ°ÑÐµÐ½Ð¸Ðµ ÑÐÐ¡Ð Ð¿ÑÐ¸ Ð¿ÑÐ¾Ð»Ð¾Ð½Ð³Ð¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð¼ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ ÑÑÐ°ÑÐµÑÑÐ¸Ñ ÐºÐ»ÐµÑÐ¾Ðº Ð½Ð° ÐÐÐ. 3. ÐÐ¿ÐµÑÐ²ÑÐµ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ ÐÐÐ, Ð´ÐµÐ¿Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¹ IGFBP3-Ð½Ð¾ÐºÐ°ÑÑÐ½ÑÐ¼Ð¸ ÑÐÐ¡Ð, ÑÐ¿Ð¾ÑÐ¾Ð±ÑÑÐ²ÑÐµÑ Ð¸Ð½Ð¸ÑÐ¸Ð°ÑÐ¸Ð¸ Ð¸ ÑÑÐºÐ¾ÑÐµÐ½Ð¸Ñ Ð¿ÑÐ¾ÑÐµÑÑÐ° Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð¹ ÑÐ¾Ð½Ð´ÑÐ¾Ð³ÐµÐ½Ð½Ð¾Ð¹ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐ¸ ÑÐÐ¡Ð.
description: The presented work is devoted to the study of the participance of ECM with different nature in the regulation of eMSCs functioning, as well as to the search for possibilities to optimize matrix deposition by cell culture. We used immunofluorescent staining of the matrix for fibronectin, collagen types I, III and IV to assess the efficiency of ECM deposition. To analyze H2O2-induced aging, we exposed eMSCs to SA-Î²-Gal staining, analysed intracellular AFC levels using the H2DCF-DA fluorescent probe, and checked p21, GAPDH expression levels and Rb phosphorylation level by Western blotting. When studying the process of chondrogenic differentiation of eMSCs we used alcian blue staining, immunophenotyping as well as RNA isolation followed by qRT-PCR. Based on our results, we found that ficoll 400 and hypoxic conditions enhance the deposition of ECM together and separately. ECM from young MSCs WJ-1 inhibited H2O2-induced aging of cells seeded on it. ECM produced by IGFBP3-knockout eMSCs accelerates the process of induced chondrogenic differentiation of eMSCs. The presented work touches upon the topical issues in the field of ECM biology. The obtained data allow us to expand our understanding of the "matrix-cell" interaction and the ways to increase the productivity of VSMCs production. Summarizing our results, we can make the following conclusions: 1. Hypoxia and macromolecular crowder ficoll 400 increase the efficiency of ECM deposition by endometrial MSCs both separately and when used together. 2. For the first time, the ability of ECM derived from young WJ-1 MSCs to partially reverse/inhibit stress-induced senescence of eMSCs during prolonged cultivation of aging cells on ECM was demonstrated. 3. For the first time it was shown that ECM deposited by IGFBP3-knockout eMSCs promotes initiation and acceleration of the process of induced chondrogenic differentiation of eMSCs.