title: ÐÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ ÐºÑÐ¸ÑÑÐ°Ð»Ð»Ð¾Ð³ÑÐ°ÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð´Ð»Ñ ÑÑÑÑÐºÑÑÑÐ½ÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ SH3 Ð´Ð¾Ð¼ÐµÐ½Ð° Ð±ÐµÐ»ÐºÐ° Grb2 Ð¸ ÑÐ¸ÑÐ¾Ð¿Ð»Ð°Ð·Ð¼Ð°ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð´Ð¾Ð¼ÐµÐ½Ð° ÑÐ¸Ð³Ð¼Ð°-1 ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Crystallographic methods applied in structural research of Grb2 SH3 domain and CTD domain of sigma-1 receptor
Creators: name: ÐÐ¾Ð»Ð³Ð¾Ð², ÐÐ»ÐµÐºÑÐ°Ð½Ð´Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: ÐÐµÐ»ÐºÐ¸
ÐÐ»ÐµÑÐºÐ° (Ð±Ð¸Ð¾Ð».)
ÑÐ¸Ð³Ð¼Ð°-1 ÑÐµÑÐµÐ¿ÑÐ¾Ñ
ÑÐ¸ÑÐ¾Ð¿Ð»Ð°Ð·Ð¼Ð°ÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð´Ð¾Ð¼ÐµÐ½
grb2
sh3 Ð´Ð¾Ð¼ÐµÐ½
n-ÐºÐ¾Ð½ÑÐµÐ²Ð¾Ð¹ sh3
n-src Ð¿ÐµÑÐ»Ñ
ÐºÑÐ¸ÑÑÐ°Ð»Ð»Ð¸Ð·Ð°ÑÐ¸Ñ
ÑÑÑÑÐºÑÑÑÐ½ÑÐµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ

description: Ð ÑÐ°Ð±Ð¾ÑÐµ Ð¿ÑÐ¾Ð²Ð¾Ð´ÑÑÑÑ ÐºÑÐ¸ÑÑÐ°Ð»Ð»Ð¾Ð³ÑÐ°ÑÐ¸ÑÐµÑÐºÐ¸Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð´Ð²ÑÑ ÑÐµÐºÐ¾Ð¼Ð±Ð¸Ð½Ð°Ð½ÑÐ½ÑÑ Ð±ÐµÐ»ÐºÐ¾Ð²: ÑÐ¸ÑÐ¾Ð¿Ð»Ð°Ð·Ð¼Ð°ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð´Ð¾Ð¼ÐµÐ½Ð° ÑÐ¸Ð³Ð¼Ð°-1 ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° Ð¸ N-ÐºÐ¾Ð½ÑÐµÐ²Ð¾Ð³Ð¾ SH3 Ð´Ð¾Ð¼ÐµÐ½Ð° Ð±ÐµÐ»ÐºÐ° Grb2. ÐÑÐ¸ÑÑÐ°Ð»Ð»Ð¸Ð·Ð°ÑÐ¸Ñ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐµÑÑÑ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Â«Ð²Ð¸ÑÑÑÐµÐ¹ ÐºÐ°Ð¿Ð»Ð¸Â». Ð¡ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÑÑÐ¾Ð¼Ð°ÑÐ¾Ð³ÑÐ°ÑÐ¸Ð¸ Ð²ÑÑÐ¾ÐºÐ¾Ð³Ð¾ ÑÐ°Ð·ÑÐµÑÐµÐ½Ð¸Ñ Ð¿Ð¾ÐºÐ°Ð·Ð°-Ð½Ð¾, ÑÑÐ¾ ÑÐµÐºÐ¾Ð¼Ð±Ð¸Ð½Ð°Ð½ÑÐ½ÑÐ¹ ÑÐ¸ÑÐ¾Ð¿Ð»Ð°Ð·Ð¼Ð°ÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð´Ð¾Ð¼ÐµÐ½ ÑÐ¸Ð³Ð¼Ð°-1 ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° ÑÑ-ÑÐµÑÑÐ²ÑÐµÑ Ð² ÑÐ°ÑÑÐ²Ð¾ÑÐµ Ð² Ð²Ð¸Ð´Ðµ ÑÐ¼ÐµÑÐ¸ Ð¼Ð¾Ð½Ð¾Ð¼ÐµÑÐ½Ð¾Ð¹ Ð¸ Ð½ÐµÑÐºÐ¾Ð»ÑÐºÐ¸Ñ Ð¾Ð»Ð¸Ð³Ð¾Ð¼ÐµÑÐ½ÑÑ ÑÐ¾ÑÐ¼; ÐºÑÐ¸ÑÑÐ°Ð»Ð»Ð¸Ð·Ð°ÑÐ¸Ð¾Ð½Ð½ÑÐµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð²ÑÑÐ²Ð¸Ð»Ð¸ Ð½Ð¾Ð²ÑÐµ ÑÑÐ»Ð¾Ð²Ð¸Ñ Ð´Ð»Ñ ÐµÐ³Ð¾ ÐºÑÐ¸ÑÑÐ°Ð»Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸. ÐÐ¾Ð»ÑÑÐµÐ½Ð° ÑÑÑÑÐºÑÑÑÐ° N-ÐºÐ¾Ð½ÑÐµÐ²Ð¾Ð³Ð¾ SH3 Ð´Ð¾Ð¼ÐµÐ½Ð° Ð±ÐµÐ»ÐºÐ° Grb2 Ñ Ð±Ð¾Ð»ÐµÐµ Ð²ÑÑÐ¾ÐºÐ¸Ð¼ ÑÐ°Ð·ÑÐµÑÐµÐ½Ð¸ÐµÐ¼ (2,5 Ã Ð² ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ð¸ Ñ 3,1 Ã). ÐÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð° ÐºÑÐ¸ÑÑÐ°Ð»Ð»Ð¸ÑÐµÑÐºÐ°Ñ ÑÑÑÑÐºÑÑÑÐ° n-Src Ð¿ÐµÑÐ»Ð¸, Ð¾ÑÑÑÑÑÑÐ²ÑÑÑÐ°Ñ Ð² Ð¿ÑÐµÐ´ÑÐ´ÑÑÐ¸Ñ ÑÑÑÑÐºÑÑÑÐ°Ñ. ÐÐ¾Ð»ÑÑÐµÐ½Ñ Ð½Ð¾Ð²ÑÐµ Ð´Ð°Ð½Ð½ÑÐµ Ð¾ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ð¸ N-ÐºÐ¾Ð½ÑÐµÐ²Ð¾Ð³Ð¾ SH3 Ð´Ð¾Ð¼ÐµÐ½Ð° Ñ Ð»Ð¸Ð³Ð°Ð½Ð´Ð°Ð¼Ð¸.
description: This thesis provides the information about crystallographic research of two recombinant proteins: cytoplasmic domain of sigma-1 receptor and N-terminal SH3 domain of Grb2. The crystallization was performed using the hanging drop technique. By means of high-resolution liquid chromatography it was shown that in saluted state recombinant CTD of sigma-1 receptor exists as a mixture of mono- and oligomers. The crystallographic research shown some new conditions for crystallization of sigma-1 receptor. The structure of N-terminal SH3 domain of Grb2 was successfully solved at higher resolution of 2.5 Ã (compared to pre-viously reported 3.1 Ã structure). The crystal structure of n-Src loop, which was absent in previously deposited structure, was resolved. Also, some new data about ligand interaction of N-terminal SH3 domain of Grb2 was found.