Additional file 1 of An NADPH oxidase regulates carbon metabolism and the cell cycle during root nodule symbiosis in common bean (Phaseolus vulgaris)
Creators: name: Fonseca-García, Citlali
name: Nava, Noreide
name: Lara, Miguel
name: Quinto, Carmen
Publisher: name: figshare
Year: 2021
Subjects: subject: Plant Biology
subject: FOS: Biological sciences

description: Additional file 1: Figure S1. Expression profiles of genes related to the isoflavonoid biosynthesis pathway in legumes. DEGs encoding proteins related to isoflavonoid biosynthesis in control and PvRbohB-RNAiP. vulgaris roots at 7 dpi with R. tropici, together with their orthologous genes from wild-type L. japonicus roots inoculated with M. loti at 7, as well as M. truncatula nodules at 6 dpi with S. meliloti. The color bars represent the log2(fold change), with red representing upregulated genes and blue representing downregulated genes. A cut-off threshold of log2FC ≥ 1.5, Padj/FDR ≤ 0.05 was used. Figure S2. DEGs related to fixed ammonia assimilation and transmembrane transporters in nodules. The heatmap shows the DEGs from control and PvRbohB-RNAi P. vulgarisroots at 7 dpi with R. tropici, together with their orthologous genes from wild-typeL. japonicus roots inoculated with M. loti at 7 dpi, as well as M. truncatula nodules at 6 dpi with S. meliloti. The color bars represent the log2(fold change), with red representing upregulated genes and blue representing downregulated genes. A cut-off threshold of log2FC ≥ 1.5, Padj/FDR ≤ 0.05 was used. Figure S3. Heatmap of the expression patterns of genes related to ureide biosynthesis. DEGs encoding proteins related to ureide metabolism in control and PvRbohB-RNAi P. vulgaris roots at 7 dpi with rhizobia. Their respective orthologous genes from wild-type, L. japonicus roots inoculated with M. loti at 7dpi were included in the analysis, along with M. truncatula nodules at 6 dpi with S. meliloti. The color bars represent the log2(fold change), with red representing upregulated genes and blue representing downregulated genes. A cut-off threshold of log2FC ≥ 1.5, Padj/FDR ≤ 0.05 was used. Figure S4. DEGs related to the oxidative and non-oxidative phases of the Pentose Phosphate Pathway (PPP). Data were analyzed in control and PvRbohB-RNAi P. vulgaris roots at 7 dpi with rhizobia. Their respective orthologous genes from wild-type L. japonicus roots inoculated with M. loti at 7 dpi were included in the analysis, along with M. truncatula nodules at 6 dpi with S. meliloti. The color bars represent the log2(fold change), with red representing upregulated genes and blue representing downregulated genes. A cut-off threshold of log2FC ≥ 1.5, Padj/FDR ≤ 0.05 was used. Figure S5. Expression profiles of genes related to sucrose catabolism to provide carbon skeletons to nodules. DEGs related to sucrose catabolism in control and PvRbohB-RNAi P. vulgaris roots at 7 dpi with rhizobia; their orthologous genes from wild-typeL. japonicus roots inoculated with M. loti at 7were including in the analysis, along with M. truncatula nodules at 6 dpi with S. meliloti. The color bars represent the log2(fold change), with red representing upregulated genes and blue representing downregulated genes. A cut-off threshold of log2FC ≥ 1.5, Padj/FDR ≤ 0.05 was used. Figure S6. DEGs involved in regulating the cell cycle in control and PvRbohB-RNAi P. vulgaris roots. Their respective orthologous genes from wild-type roots of L. japonicus inoculated with M. loti at 7 dpi were included in the analysis, along with M. truncatula nodules at 6 dpi with S. meliloti The color bars represent the log2(fold change) of the DEG, with red and blue representing upregulated and downregulated genes, respectively. A cut-off threshold of log2FC ≥ 1.5, Padj/FDR ≤ 0.05 was used.