cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
sj-pdf-1-tra-10.1177_1460408620968340 - Supplemental material for Psychosocial impacts of training to provide professional help: Harm and growth
Creators: name: Ball, Jacqueline
name: Watsford, Clare
name: Scholz, Brett
Publisher: name: SAGE Journals
Year: 2020
Subjects: subject: 111708 Health and Community Services
subject: FOS: Health sciences
subject: 110305 Emergency Medicine
subject: FOS: Clinical medicine

description: Supplemental material, sj-pdf-1-tra-10.1177_1460408620968340 for Psychosocial impacts of training to provide professional help: Harm and growth by Jacqueline Ball, Clare Watsford and Brett Scholz in Trauma
Guidance relevant to the reporting of health equity in observational research: a scoping review protocol
Creators: name: Rizvi, Anita
name: Lawson, Daeria O
name: Young, Taryn
name: Dewidar, Omar
name: Nicholls, Stuart
name: Akl, Elie A
name: Little, Julian
name: Magwood, Olivia
name: Shamseer, Larissa
name: Ghogomu, Elizabeth
name: Jull, Janet Elizabeth
name: Rader, Tamara
name: Bhutta, Zulfiqar
name: Chamberlain, Catherine
name: Ellingwood, Holly
name: Greer-Smith, Regina
name: Hardy, Billie-Jo
name: Harwood, Matire
name: Kennedy, Michelle
name: Kredo, Tamara
name: Loder, Elizabeth
name: Mahande, Michael Johnson J
name: Mbuagbaw, Lawrence
name: Nkangu, Miriam
name: Okwen, Patrick M
name: Ramke, Jacqueline
name: Tufte, Janice
name: Tugwell, Peter
name: Wang, Xiaoqin
name: Wiysonge, Charles Shey
name: Welch, Vivian A
Publisher: name: La Trobe
Year: 2022
Subjects: classificationCode: 420699
subject: Public health not elsewhere classified

description: INTRODUCTION: Health inequities are defined as unfair and avoidable differences in health between groups within a population. Most health research is conducted through observational studies, which are able to offer real-world insights about etiology, healthcare policy/programme effectiveness and the impacts of socioeconomic factors. However, most published reports of observational studies do not address how their findings relate to health equity. Our team seeks to develop equity-relevant reporting guidance as an extension of the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) statement. This scoping review will inform the development of candidate items for the STROBE-Equity extension. We will operationalise equity-seeking populations using the PROGRESS-Plus framework of sociodemographic factors. As part of a parallel stream of the STROBE-Equity project, the relevance of candidate guideline items to Indigenous research will be led by Indigenous coinvestigators on the team. METHODS AND ANALYSIS: We will follow the Joanna Briggs Institute method for conducting scoping reviews. We will evaluate the extent to which the identified guidance supports or refutes our preliminary candidate items for reporting equity in observational studies. These candidate items were developed based on items from equity-reporting guidelines for randomised trials and systematic reviews, developed by members of this team. We will consult with our knowledge users, patients/public partners and Indigenous research steering committee to invite suggestions for relevant guidance documents and interpretation of findings. If the identified guidance suggests the need for additional candidate items, they will be developed through inductive thematic analysis. ETHICS AND DISSEMINATION: We will follow a principled approach that promotes ethical codevelopment with our community partners, based on principles of cultural safety, authentic partnerships, addressing colonial structures in knowledge production and the shared ownership, interpretation, and dissemination of research. All products of this research will be published as open access.
Computational Screening of Anti-Cancer Drugs Identifies a New BRCA Independent Gene Expression Signature to Predict Breast Cancer Sensitivity to Cisplatin
Creators: name: Berthelet, Jean
name: Foroutan, Momeneh
name: Bhuva, Dharmesh D
name: Whitfield, Holly J
name: El-Saafin, Farrah
name: Cursons, Joseph
name: Serrano, Antonin
name: Merdas, Michal
name: Lim, Elgene
name: Charafe-Jauffret, Emmanuelle
name: Ginestier, Christophe
name: Ernst, Matthias
name: Hollande, Frederic
name: Anderson, Robin
name: Pal, Bhupinder
name: Yeo, Belinda
name: Davis, Melissa J
name: Merino, Delphine
Publisher: name: La Trobe
Year: 2022
Subjects: classificationCode: 321199
subject: Oncology and carcinogenesis not elsewhere classified
classificationCode: 321499
subject: Pharmacology and pharmaceutical sciences not elsewhere classified

description: The development of therapies that target specific disease subtypes has dramatically improved outcomes for patients with breast cancer. However, survival gains have not been uniform across patients, even within a given molecular subtype. Large collections of publicly available drug screening data matched with transcriptomic measurements have facilitated the development of computational models that predict response to therapy. Here, we generated a series of predictive gene signatures to estimate the sensitivity of breast cancer samples to 90 drugs, comprising FDA-approved drugs or compounds in early development. To achieve this, we used a cell line-based drug screen with matched transcriptomic data to derive in silico models that we validated in large independent datasets obtained from cell lines and patient-derived xenograft (PDX) models. Robust computational signatures were obtained for 28 drugs and used to predict drug efficacy in a set of PDX models. We found that our signature for cisplatin can be used to identify tumors that are likely to respond to this drug, even in absence of the BRCA-1 mutation routinely used to select patients for platinum-based therapies. This clinically relevant observation was confirmed in multiple PDXs. Our study foreshadows an effective delivery approach for precision medicine.
Additional file 2 of Factors associated with anemia among children in South and Southeast Asia: a multilevel analysis
Creators: name: Sunuwar, Dev Ram
name: Singh, Devendra Raj
name: Pradhan, Pranil Man Singh
name: Shrestha, Vintuna
name: Rai, Pushpa
name: Shah, Sunil Kumar
name: Adhikari, Bipin
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Biotechnology
subject: Sociology
subject: FOS: Sociology
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Science Policy
subject: Hematology

description: Supplementary Material 2
Diagnóstico Genético Del Polimorfismo En Citocromo P450 Mediante Ensayo De Pcr Múltiplex (Práctica En Laboratorio Virtual Cibertorio)
Creators: name: Herráez, Angel
Publisher: name: Zenodo
Year: 2017
Subjects: CYP
citocromo P450
medicina personalizada
polimorfismo
PCR
laboratorio virtual
simulación
in silico
diversidad genética
respuesta a fármacos

description: Guión de trabajo para el laboratorio virtual «Cibertorio» en Biomodel.UAH.es

Se habla de diversidad genética de los individuos como el resultado de que, en diversas regiones del genoma, unas personas tenemos secuencias de nucleótidos que nos diferencian de otras; esto se denomina polimorfismo genético. Cuando estas regiones polimórficas corresponden a zonas codificantes de un producto, existen consecuencias funcionales.

La superfamilia enzimática citocromo P450 juega un papel crucial en el metabolismo de numerosos fármacos y de otros compuestos xenobióticos. El polimorfismo existente en los genes de estas enzimas hace que la respuesta a los fármacos sea diferente entre individuos.

En concreto, este ensayo se centra en el polimorfismo genético de la isoforma CYP2C9. La diferencia entre los alelos presentes en distintos individuos puede detectarse utilizando PCR con cebadores específicos para cada alelo. A continuación, los segmentos amplificados se analizan mediante electroforesis.

Objetivo: Empleando cebadores específicos para algunos de los alelos más frecuentes, analizar el genotipo de varios pacientes. Se parte de muestras de DNA de los pacientes, se realiza la amplificación por PCR múltiplex y se separan los segmentos amplificados mediante electroforesis.
Outcomes of peripherally inserted central catheter vs conventional central venous catheters in hematological cancer patients: a systematic review and meta-analysis
Creators: name: Ge, Weilei
name: Zheng, Chen
Publisher: name: Taylor & Francis
Year: 2025
Subjects: Medicine
Environmental Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Cancer
Science Policy

description: This review aimed to examine if there is any difference in the risk of thrombosis and central line-associated bloodstream infection (CLABSI) with the use of peripherally inserted central catheter (PICC) and conventional central venous catheters (CVC) in hematological cancer patients. We searched the online databases of PubMed, CENTRAL, Scopus, Web of Science, and Embase for all types of studies comparing the risk of thrombosis and CLABSI between PICC and CVC. The search ended on 23rd September 2024. Eight studies were included. One was a randomized trial while others were observational studies. Meta-analysis showed no statistically significant difference in the risk of thrombosis between PICC and CVC (OR: 1.69 95% CI: 0.75, 3.82 I2 = 78%). However, these results were not stable on sensitivity analysis. The exclusion of two studies indicated a higher risk of thrombosis with PICC. Pooled analysis showed that the risk of CLABSI was significantly lower with PICC as compared to CVC (OR: 0.52 95% CI: 0.40, 0.66 I2 = 0%). Results of subgroup analysis based on study design and diagnosis showed conflicting results. There is conflicting evidence on the risk of thrombosis between PICC and CVC when used for hematological cancer patients. There could be a tendency of higher risk of thrombosis with PICC which needs to be confirmed by further studies. However, the use of PICC may reduce the risk of CLABSI in such patients. The quality of evidence is low owing to the predominance of observational studies with high inter-study heterogeneity.
Additional file 1 of Cardiopulmonary exercise testing before and after intravenous iron in preoperative patients: a prospective clinical study
Creators: name: Plumb, James O. M.
name: Otto, James M.
name: Kumar, Shriya B.
name: Bali, Sitara
name: Wakatsuki, Mai
name: Schmidt, Walter F. J.
name: Montgomery, Hugh E.
name: Grocott, Michael P. W.
name: Levett, Denny Z.
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Neuroscience
subject: Physiology
subject: FOS: Biological sciences
subject: Biotechnology
subject: Immunology
subject: FOS: Clinical medicine
subject: Science Policy
subject: Mental Health
subject: Hematology
subject: Infectious Diseases
subject: FOS: Health sciences

description: Additional file 1.
sj-pdf-3-jcb-10.1177_0271678X20964248 - Supplemental material for Kinetics and 28-day test–retest repeatability and reproducibility of [11C]UCB-J PET brain imaging
Creators: name: Tuncel, Hayel
name: Boellaard, Ronald
name: Coomans, Emma M
name: de Vries, Erik FJ
name: Glaudemans, Andor WJM
name: Feltes, Paula Kopschina
name: García, David V
name: Verfaillie, Sander CJ
name: Wolters, Emma E
name: Sweeney, Steven P
name: Ryan, J Michael
name: Ivarsson, Magnus
name: Lynch, Berkley A
name: Schober, Patrick
name: Scheltens, Philip
name: Schuit, Robert C
name: Windhorst, Albert D
name: De Deyn, Peter P
name: van Berckel, Bart NM
name: Golla, Sandeep SV
Publisher: name: SAGE Journals
Year: 2020
Subjects: subject: Emergency Medicine
subject: Endocrinology
subject: Medicine
subject: Neurology and Neuromuscular Diseases
subject: FOS: Clinical medicine
subject: Radiology and Organ Imaging
subject: Cell Biology
subject: Biochemistry
subject: Biological Sciences not elsewhere classified
subject: Neuroscience

description: Supplemental material, sj-pdf-3-jcb-10.1177_0271678X20964248 for Kinetics and 28-day test–retest repeatability and reproducibility of [11C]UCB-J PET brain imaging by Hayel Tuncel, Ronald Boellaard, Emma M Coomans, Erik FJ de Vries, Andor WJM Glaudemans, Paula Kopschina Feltes, David V García, Sander CJ Verfaillie, Emma E Wolters, Steven P Sweeney, J Michael Ryan, Magnus Ivarsson, Berkley A Lynch, Patrick Schober, Philip Scheltens, Robert C Schuit, Albert D Windhorst, Peter P De Deyn, Bart NM van Berckel and Sandeep SV Golla in Journal of Cerebral Blood Flow & Metabolism
Research priorities for prehospital care of older patients with injuries: scoping review
Creators: name: Harthi, Naif
name: Goodacre, Steve
name: Sampson, Fiona
name: Alharbi, Rayan Jafnan M
Publisher: name: La Trobe
Year: 2022
Subjects: classificationCode: 420699
subject: Public health not elsewhere classified

description: BACKGROUND AND OBJECTIVE: There is increasing recognition of the importance of prehospital trauma care for older patients, but little systematic research to guide practice. We aimed to review the published evidence on prehospital trauma care for older patients, determine the scope of existing research and identify research gaps in the literature. METHODS: We undertook a systematic scoping review guided by the Arksey and O'Malley framework and reported in line with the PRISMA-ScR checklist. A systematic search was conducted of Scopus, CINAHL, MEDLINE, PubMed and Cochrane library databases to identify articles published between 2001 and 2021. Study selection criteria were applied independently by two reviewers. Data were extracted, charted and summarised from eligible articles. A data-charting form was then developed to facilitate thematic analysis. Narrative synthesis then involved identifying major themes and subthemes from the data. RESULTS: We identified and reviewed 65 studies, and included 25. We identified five categories: 'field triage', 'ageing impacts', 'decision-making', 'paramedic' awareness' and 'paramedic's behaviour'. Undertriage and overtriage (sensitivity and specificity) were commonly cited as poorly investigated field-triage subthemes. Ageing-related physiologic changes, comorbidities and polypharmacy were the most widely researched. Inaccurate decision-making and poor early identification of major injuries were identified as potentially influencing patient outcomes. CONCLUSION: This is the first study reviewing the published evidence on prehospital trauma care for older patients and identifying research priorities for future research. Field-triage tools, paramedics' knowledge about injuries in the older population, and understanding of paramedics' negative behaviours towards older patients were identified as key research priorities.
Additional file 1 of Sick and depressed? The causal impact of a diabetes diagnosis on depression
Creators: name: Gaggero, Alessio
name: Gil, Joan
name: Jiménez-Rubio, Dolores
name: Zucchelli, Eugenio
Publisher: name: figshare
Year: 2024
Subjects: Medicine
Biotechnology
Chemical Sciences not elsewhere classified
Mental Health

description: Additional file 1: Table S1. Summary Statistics - By T2DM Diagnosis Status. Table S2. Summary Statistics - By Gender. Table S3. Summary Statistics - By Weight Loss Status. Table S4. RDD Estimates of the Impact of a T2DM Diagnosis on Depression - Different Cut-offs. Table S5. RDD Estimates of the Impact of a T2DM Diagnosis on Depression – Different Polynomials. Table S6. Fuzzy RDD Estimates - Non-Parametric Approach.
sj-docx-1-nnr-10.1177_1545968320961114 – Supplemental material for A Music-Based Digital Therapeutic: Proof-of-Concept Automation of a Progressive and Individualized Rhythm-Based Walking Training Program After Stroke
Creators: name: Hutchinson, Karen
name: Sloutsky, Regina
name: Collimore, Ashley
name: Adams, Benjamin
name: Harris, Brian
name: Ellis, Terry D.
name: Awad, Louis N.
Publisher: name: SAGE Journals
Year: 2023
Subjects: subject: 110308 Geriatrics and Gerontology
subject: FOS: Clinical medicine
subject: 110904 Neurology and Neuromuscular Diseases
subject: Neuroscience

description: Supplemental material, sj-docx-1-nnr-10.1177_1545968320961114 for A Music-Based Digital Therapeutic: Proof-of-Concept Automation of a Progressive and Individualized Rhythm-Based Walking Training Program After Stroke by Karen Hutchinson, Regina Sloutsky, Ashley Collimore, Benjamin Adams, Brian Harris, Terry D. Ellis and Louis N. Awad in Neurorehabilitation and Neural Repair
FORMS OF WORKING WITH PARENTS IN PRIMARY CLASSES
Creators: name: Kudyarova Artikgul
Publisher: name: Zenodo
Year: 2024

description: Abstract. In this article, the forms of working with parents in elementary grades, organizing a meeting of parents, creating and conducting activities of the class parents' council, working with children with difficult upbringing, and working with students' families are used in this article. traditional and non-traditional methods are discussed.

Kеywоrds: Primary education, student, class leader, parent, parent meeting, parent council, method, traditional and non-traditional methods.
Perceived Work Ability during Enforced Working from Home Due to the COVID-19 Pandemic among Finnish Higher Educational Staff
Creators: name: Kyronlahti, Saila
name: Neupane, Subas
name: Nygard, Clas-Hakan
name: Oakman, Jodi
name: Juutinen, Soile
name: Makikangas, Anne
Publisher: name: La Trobe
Year: 2022
Subjects: classificationCode: 529999
subject: Other psychology not elsewhere classified

description: Background: Due to COVID-19 pandemic, many employees were forced to suddenly shift to working from home (WFH). How this disruption of work affected employees' work ability is not known. In this study, we investigated the developmental profiles of work ability among Finnish higher education employees in a one-year follow-up during the enforced WFH. Secondly, we investigated demographic, organizational, and ergonomic factors associated with the developmental profiles. Methods: A longitudinal web-survey was conducted with four measurement points (April 2020-February 2021). Employees of a Finnish university who answered the questionnaire at baseline and at least at two follow-up surveys (n = 678) were included (71% women, 45% teachers/research staff, 44% supporting staff, 11% hired students). Perceived work ability was measured on a scale of 1-5 in all timepoints. Latent class growth curve analysis was used to identify profiles of work ability. Multinomial logistic regression was used to determine the associations of demographic factors, perceived stress, musculoskeletal pain, functionality of home for work, and organizational support with the work ability profiles. Results: Six distinct work ability profiles were identified. For most (75%), work ability remained stable during the follow-up. A total of 17% had a favourable trend (very good-stable or increasing) of work ability, and 8% had non-favourable (poor-stable or decreasing). Poor ergonomics at home, low organizational support, high stress, and musculoskeletal pain were associated with non-favourable development of work ability. Conclusions: Heterogeneity in development of work ability during forced WFH was found. Several factors were identified through which work ability can be supported.
Downregulated expression of dual-specificity phosphatase-1 in multiple myeloma as a predictor of poor survival outcomes
Creators: name: Guo, Dan
name: Lu, Jinfeng
name: Hong, Lemin
name: Liu, Hong
name: Huang, Hongming
Publisher: name: Taylor & Francis
Year: 2025
Subjects: subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Hematology

description: Multiple myeloma (MM) is an incurable hematological malignancy, Dual-specificity phosphatase-1 (DUSP1) plays a crucial role in the initiation and progression of various tumors. Here, we aim to elucidate the role of DUSP1 in MM. DUSP1 mRNA expression was analyzed based on public datasets, and protein expression was determined by immunohistochemistry. The association between DUSP1 and clinicopathological characteristics, as well as its impact on survival, were investigated. Protein–protein interaction and gene set enrichment analysis were performed. Low DUSP1 expression was detected in MM and it was associated with elevated β2-microglobulin, C-reactive protein, creatinine, lactate dehydrogenase, plasma cell ratio, and decreased hemoglobin levels. The DUSP1high group exhibited superior outcomes across clinical endpoints. Univariate and multivariate analyses indicated that low DUSP1 expression was an independent prognostic factor for poor OS (hazard ratio = 0.273). The findings suggested that DUSP1 expression was related to proto-oncogene c-Fos (FOS), heat shock protein family member 1a (HSPA1A), several members of the MAPK family, nuclear receptor subfamily 3, group C, member 1 (NR3C1), and zinc finger protein 36 (ZFP36). DUSP1 mRNA levels were positively correlated with ribosomes and were negatively correlated with oocyte meiosis, one carbon pool by folate, homologous recombination, base excision repair, and pyrimidine metabolism pathways. The potential mechanisms identified through the PPI network analysis could provide insight into how DUSP1 may influence MM. Low expression of DUSP1 may be considered a poor prognostic factor for MM patients.
DNA Damage, Defective DNA Repair, and Neurodegeneration in Amyotrophic Lateral Sclerosis
Creators: name: Konopka, A
name: Atkin, Julie
Publisher: name: La Trobe
Year: 2022
Subjects: classificationCode: 310199
subject: Biochemistry and cell biology not elsewhere classified
classificationCode: 310599
subject: Genetics not elsewhere classified
classificationCode: 320999
subject: Neurosciences not elsewhere classified

description: DNA is under constant attack from both endogenous and exogenous sources, and when damaged, specific cellular signalling pathways respond, collectively termed the “DNA damage response.” Efficient DNA repair processes are essential for cellular viability, although they decline significantly during aging. Not surprisingly, DNA damage and defective DNA repair are now increasingly implicated in age-related neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS). ALS affects both upper and lower motor neurons in the brain, brainstem and spinal cord, leading to muscle wasting due to denervation. DNA damage is increasingly implicated in the pathophysiology of ALS, and interestingly, the number of DNA damage or repair proteins linked to ALS is steadily growing. This includes TAR DNA binding protein 43 (TDP-43), a DNA/RNA binding protein that is present in a pathological form in almost all (97%) cases of ALS. Hence TDP-43 pathology is central to neurodegeneration in this condition. Fused in Sarcoma (FUS) bears structural and functional similarities to TDP-43 and it also functions in DNA repair. Chromosome 9 open reading frame 72 (C9orf72) is also fundamental to ALS because mutations in C9orf72 are the most frequent genetic cause of both ALS and related condition frontotemporal dementia, in European and North American populations. Genetic variants encoding other proteins involved in the DNA damage response (DDR) have also been described in ALS, including FUS, SOD1, SETX, VCP, CCNF, and NEK1. Here we review recent evidence highlighting DNA damage and defective DNA repair as an important mechanism linked to neurodegeneration in ALS.
Theodote, and Anthimos, Leontios and Euprepios (mother and brothers of Kosmas and Damianos)
Creators: name: Admin, CSLA
Publisher: name: University of Oxford
Year: 2017
Subjects: History

description: All evidence related to Theodote, and Anthimos, Leontios and Euprepios (mother and brothers of Kosmas and Damianos).
Additional file 1 of Reduction of oxytocin-containing neurons and enhanced glymphatic activity in the hypothalamic paraventricular nucleus of patients with type 2 diabetes mellitus
Creators: name: Correa-da-Silva, Felipe
name: Kalsbeek, Martin J.
name: Gadella, Femke S.
name: Oppersma, Jorn
name: Jiang, Wei
name: Wolff, Samantha E. C.
name: Korpel, Nikita L.
name: Swaab, Dick F.
name: Fliers, Eric
name: Kalsbeek, Andries
name: Yi, Chun-Xia
Publisher: name: figshare
Year: 2024
Subjects: subject: Cell Biology
subject: Molecular Biology
subject: Neuroscience
subject: Physiology
subject: FOS: Biological sciences
subject: Mental Health

description: Additional file 1. List of antibodies for immunohistochemestry and immunofluorescence and detailed statistical information.
Diagnóstico Genético Molecular Del Polimorfismo De Globina Beta S Mediante Rflp (Práctica En Laboratorio Virtual Cibertorio)
Creators: name: Herráez, Angel
Publisher: name: Zenodo
Year: 2017
Subjects: globina S
hemoglobina
falciforme
drepanocitosis
enzimas de restricción
RFLP
polimorfismo
laboratorio virtual
simulación
in silico

description: Guión de trabajo para el laboratorio virtual «Cibertorio» en Biomodel.UAH.es

La anemia drepanocítica (anemia de células falciformes) se origina por la mutación de un solo nucleótido en el gen de globina beta humana. Esto genera en la población un polimorfismo, definido por la presencia del alelo normal o el mutado, en cada uno de los dos cromosomas 11 homólogos.

En esta práctica se pretende el diagnóstico genético de esta enfermedad en muestras de varios pacientes a través de un polimorfismo RFLP, es decir, mediante restricción y electroforesis.

Objetivo: Empleando una enzima de restricción elegida previamente, que corta de forma diferente los alelos normal y mutado del gen de globina beta, realizar el diagnóstico genético de varios pacientes.
Secuenciación De Dna Para Analizar El Polimorfismo De Globina Beta S (Práctica En Laboratorio Virtual Cibertorio)
Creators: name: Herráez, Angel
Publisher: name: Zenodo
Year: 2017
Subjects: globina S
hemoglobina
falciforme
drepanocitosis
secuenciación
didesoxi
polimorfismo
laboratorio virtual
simulación
in silico

description: Guión de trabajo para el laboratorio virtual «Cibertorio» en Biomodel.UAH.es

La anemia drepanocítica (anemia de células falciformes) se origina por la mutación de un solo nucleótido en el gen de globina beta humana. Esto genera en la población un polimorfismo, definido por la presencia del alelo normal o el mutado, en cada uno de los dos cromosomas 11 homólogos.

En esta práctica se pretende el diagnóstico genético de esta enfermedad mediante secuenciación de la región genómica correspondiente a este gen.

Objetivo: Verificar la diferencia entre las dos variantes del gen de globina, una normal y otra responsable de la drepanocitosis, a la vez que se ilustra cuál es el resultado de la técnica enzimática de secuenciación del DNA (método didesoxi, de terminación de cadena o de Sanger).

El laboratorio virtual Cibertorio realiza el análisis electroforético de las mezclas de reacción de Sanger en las que se emplea un nucleótido marcado con fósforo radiactivo o con un fluorocromo, o bien los 4 didesoxirribonucleótidos marcados con 4 fluorocromos distintos.
Schema Therapy as an effective treatment for Borderline Personality Disorder (BPD): models and guidelines
Creators: name: Gordon, Anna
Publisher: name: Humanities Commons
Year: 2021
Subjects: subject: Borderline personality disorder

description: Patients diagnosed with border line personality disorder (also known as BPD) were historically recognized as being among the most challenging patients. They were considered to be patients who either did not respond well to treatment or, best-case scenario, did not respond well to treatment at all. They could not be assisted by therapy. In the meanwhile, they have significant needs for both physical and mental health care, and their rates of withdrawal from rehabilitation programs are rather high. The relatively recent cognitive-based integrative psychotherapy known as schema therapy is an excellent method for treating borderline personality disorder. This therapy is based on cognitive models (BPD). In comparison to psychodynamic therapy and treatment as usual, its efficacy and cost-effectiveness have been shown by several studies and are now well established. In addition, there is a continuously low rate of patient dropout from treatment, which indicates that schema psychotherapy is highly accepted by patients. The authors of this research provide therapists with a useful guidance for doing schema therapy with individuals suffering from BPD. It provides a conceptual model of borderline personality disorder (BPD), a treatment model, as well as a wealth of approaches and strategies for treating borderline personality disorder patients. It does this by building upon Jeffrey Young's schema mode model and Young's schema therapy, as well as deep insight from Beckian talk restructuring and experiential methods.
Análisis De Paternidad Mediante Ensayo De Pcr Múltiplex Sobre Marcadores Polimórficos Str (Práctica En Laboratorio Virtual Cibertorio)
Creators: name: Herráez, Angel
Publisher: name: Zenodo
Year: 2017
Subjects: polimorfismo genético
STR
CODIS
PCR
paternidad
ensayos de identidad
simulación
laboratorio virtual
in silico

description: Las pruebas de paternidad se basan en el patrón mendeliano de herencia junto con la diversidad genética de los individuos (polimorfismo genético). Este ensayo en concreto se centra en varias regiones polimórficas conocidas como STR que forman parte de uno de los grupos de marcadores de referencia internacional, los marcadores del CoDIS conocidos por ser los utilizados por el FBI. La diferencia entre los alelos presentes en distintos individuos está en el número de unidades repetidas y, en consecuencia, en la longitud de los fragmentos de DNA que se amplificarán mediante PCR y se analizarán mediante electroforesis.

Objetivo: Analizar los marcadores polimórficos para determinar cuál de los candidatos es el padre. El ensayo consiste en, partiendo de las muestras de DNA de cada uno de los padres potenciales, de la madre y del hijo/a, realizar una amplificación por PCR de varios marcadores STR y separar los fragmentos amplificados mediante electroforesis. Por comparación de los patrones de bandas, se deducirá cuál de los candidatos es el padre (o bien se descartarán los cuatro)
sj-pdf-10-jhs-10.1177_1753193420914762 - Supplemental material for BSSH Spring Meeting Abstracts
Creators: name: Lu, Justin D.
name: Muskaan Sachdeva
name: Vender, Ronald
Publisher: name: SAGE Journals
Year: 2020
Subjects: subject: 110314 Orthopaedics
subject: FOS: Clinical medicine
subject: 110604 Sports Medicine
subject: FOS: Health sciences
subject: 110323 Surgery

description: Supplemental material, sj-pdf-10-jhs-10.1177_1753193420914762 for BSSH Spring Meeting Abstracts by in Journal of Hand Surgery (European Volume)
Diagnóstico Genético De Variantes Polimórficas Asociadas A La Celiaquía, Mediante Ensayo De Pcr Múltiplex Sobre Los Marcadores Dq2 Y Dq8 (Práctica En Laboratorio Virtual Cibertorio)
Creators: name: Herráez, Angel
Publisher: name: Zenodo
Year: 2017
Subjects: polimorfismo genético
PCR
medicina personalizada
laboratorio virtual
simulación
in silico
celiaquía
enfermedad celíaca
HLA DQ2
HLA DQ8
diagnóstico genético

description: Guión de trabajo para el laboratorio virtual «Cibertorio» en Biomodel.UAH.es

Se habla de diversidad genética de los individuos como el resultado de que, en diversas regiones del genoma, unas personas tenemos secuencias de nucleótidos que nos diferencian de otras; esto se denomina polimorfismo genético. Cuando estas regiones polimórficas corresponden a zonas codificantes de un producto, existen consecuencias funcionales.

El diagnóstico y el pronóstico de la enfermedad celíaca son complejos pero incluyen como uno de los indicios el análisis del genotipo en los genes DQA1 y DQB1 (pertenecientes a la familia de antígenos leucocitarios humanos, HLA). En este ensayo se detectan las diferencias entre los alelos más comunes de estos genes polimórficos, utilizando PCR con cebadores específicos para cada alelo. A continuación, los segmentos amplificados se analizarán mediante electroforesis. Es posible así diagnosticar en las muestras los haplotipos DQ2 y DQ8 asociados a la aparición de celiaquía.

Objetivo: Empleando cebadores específicos para algunos de los alelos más frecuentes, analizar el genotipo de varios pacientes. Se parte de muestras de DNA de los pacientes, se realiza la amplificación por PCR múltiplex y se separan los segmentos amplificados mediante electroforesis.
A theory driven, pragmatic trial implementing changes to routine antenatal care that supports recommended pregnancy weight gain
Creators: name: de Jersey, Susan
name: Guthrie, Taylor
name: Callaway, Leonie
name: Tyler, Jeanette
name: New, Karen
name: Nicholson, Jan
Publisher: name: La Trobe
Year: 2022
Subjects: classificationCode: 420699
subject: Public health not elsewhere classified
classificationCode: 420499
subject: Midwifery not elsewhere classified

description: BACKGROUND: Prevention of weight gain outside recommendations is a challenge for health services, with several barriers to best practice care identified. The aim of this pragmatic implementation study with a historical control was to examine the impact of implementing a service wide education program, and antenatal care pregnancy weight gain chart combined with brief advice on women's knowledge of recommended gestational weight gain (GWG), the advice received and actual GWG. METHODS: The PRECEDE PROCEED Model of Health Program planning guided intervention and evaluation targets and an implementation science approach facilitated service changes. Pregnant women < 22 weeks' gestation attending the antenatal clinic at a metropolitan birthing hospital in Australia were recruited pre (2010, n = 715) and post (2016, n = 478) implementation of service changes. Weight measurements and questionnaires were completed at recruitment and 36 weeks' gestation. Questionnaires assessed advice received from health professionals related to healthy eating, physical activity, GWG, and at recruitment only, pre-pregnancy weight and knowledge of GWG recommendations. RESULTS: Women who correctly reported their recommended GWG increased from 34% (pre) to 53% (post) (p < 0.001). Between pre and post implementation, the advice women received from midwives on recommended GWG was significantly improved at both recruitment- and 36-weeks' gestation. For normal weight women there was a reduction in GWG (14.2 ± 5.3 vs 13.3 ± 4.7 kg, p = 0.04) and clinically important reduction in excess GWG between pre and post implementation (31% vs 24%, p = 0.035) which remained significant after adjustment (AOR 0.53 [95%CI 0.29-0.96]) (p = 0.005). CONCLUSIONS: Service wide changes to routine antenatal care that address identified barriers to supporting recommended GWG are likely to improve the care and advice women receive and prevent excess GWG for normal weight women.
Análisis De Adulteración En Productos Lácteos Mediante Ensayo De Pcr Múltiplex Sobre Marcadores De Dna Mitocondrial (Práctica En Laboratorio Virtual Cibertorio)
Creators: name: Herráez, Angel
Publisher: name: Zenodo
Year: 2017
Subjects: adulteración
lácteos
in silico
laboratorio virtual
simulación
DNA mitocondrial
PCR
diversidad genética

description: Guión de trabajo para el laboratorio virtual «Cibertorio» en Biomodel.UAH.es

La secuencia del DNA mitocondrial presenta diferencias entre especies que pueden aprovecharse para realizar un análisis molecular que demuestre la procedencia de una muestra.

En este ensayo se analizan muestras de queso supuestamente de cabra, utilizando PCR con cebadores específicos para el DNA mitocondrial de cada especie. A continuación, los segmentos amplificados se analizarán mediante electroforesis. Es posible así detectar la adulteración de la leche de cabra con leche de oveja o de vaca.

Objetivo: Empleando cebadores específicos para el DNA mitocondrial de cada especie, analizar el origen de varias muestras. Se parte de muestras de referencia de DNA de vaca, oveja y cabra, así como DNA extraido de 3 muestras de queso, se realiza la amplificación por PCR múltiplex y se separan los segmentos amplificados mediante electroforesis.
sj-pdf-1-eup-10.1177_1465116520961467 - Supplemental material for One agenda-setter or many? The varying success of policy initiatives by individual Directorates-General of the European Commission 1994–2016
Creators: name: Rauh, Christian
Publisher: name: SAGE Journals
Year: 2020
Subjects: subject: 160607 International Relations
subject: FOS: Political science
subject: 120505 Regional Analysis and Development
subject: FOS: Social and economic geography

description: Supplemental material, sj-pdf-1-eup-10.1177_1465116520961467 for One agenda-setter or many? The varying success of policy initiatives by individual Directorates-General of the European Commission 1994–2016 by Christian Rauh in European Union Politics
Introducción A Las Bases De Datos De Secuencia Genómica Y Análisis De Secuencias Relacionadas Con El Polimorfismo De Globina Beta S (Práctica En Laboratorio Virtual Cibertorio)
Creators: name: Herráez, Angel
Publisher: name: Zenodo
Year: 2017
Subjects: globina beta
hemoglobina S
drepanocitosis
falciforme
laboratorio virtual
secuencias genómicas
bases de datos
exones

description: Guión de trabajo para el laboratorio virtual «Cibertorio» en Biomodel.UAH.es

La anemia drepanocítica (anemia de células falciformes) se origina por la mutación de un solo nucleótido en el gen de globina beta humana. Esto genera en la población un polimorfismo, definido por la presencia del alelo normal o el mutado, en cada uno de los dos cromosomas 11 homólogos.

En el contexto del estudio molecular de esta enfermedad y su diagnóstico genético, vamos a estudiar las secuencias depositadas en las bases de datos del genoma correspondientes al gen de globina beta y su punto polimórfico.

Objetivo: Obtener información sobre el polimorfismo del gen de globina beta que da lugar a la hemoglobina S y causa la drepanocitosis. Familiarizarse con las bases de datos de secuencia, diferencias entre DNA genómico y cDNA, conceptos de intrón y exón.
Leitura e escrita: gênese e modernidade
Creators: name: Resende Costa, Júlio
name: Maria dos Santos, Sônia
Publisher: name: Zenodo
Year: 2019
Subjects: História da Alfabetização
Alfabetização
Letramento.

description: O tema alfabetização foi escolhido por ser um campo da educação que ainda produz grandes debates, cuja discussão parece estar longe de ser encerrada. A celeuma que se instalou nas escolas brasileiras a partir da década de 1980 deu novo vigor à discussão sobre a alfabetização, o que ensejou novos estudos e descobertas. A querela acerca dessa temática ainda está longe de ser encerrada e, por esse motivo, todo estudo que surgir deve contribuir para a ampliação do debate. O propósito central deste trabalho procurou identificar os elementos constitutivos do processo de alfabetização ao longo do tempo e seus determinantes histórico-sociais. Esta pesquisa tem como sustentação teórico metodológica a teoria de que a alfabetização é um processo complexo e que ultrapassa o simples conceito de ler e escrever e avança para o campo do letramento, ou seja, da aplicação da alfabetização em contextos reais e práticas sociais que permitam ao sujeito se orientar no espaço, ler, escrever, compreender e ser compreendido, refletir sobre o que leu, analisar, comparar, sintetizar e inferir. O percurso metodológico definido para esta pesquisa permitiu trilhar os caminhos adequados para alcançar o seu propósito investigativo. O trabalho possui cunho descritivo e abordagem qualitativa do problema de pesquisa, com base na revisão da literatura. Como resultados, observou--se que a alfabetização é um processo social e dinâmico. O processo de alfabetização reflete os contextos históricos e procura atender às demandas sociais desses momentos. Atualmente, além do domínio da tecnologia da escrita e da leitura, espera-se que a alfabetização seja construída na perspectiva do letramento. Não se pode afirmar que um método de alfabetização seja mais eficaz que o outro. Os métodos de alfabetização resultam de determinantes históricos, sociais e culturais, isto é, são dinâmicos e espelham as diligências de uma determinada sociedade em relação à concepção de alfabetização. Conhecer com profundidade a base teórica que sustenta um método de alfabetização facilita a aquisição do código escrito em seu sentido mais pleno.
Additional file 2 of Reduction of oxytocin-containing neurons and enhanced glymphatic activity in the hypothalamic paraventricular nucleus of patients with type 2 diabetes mellitus
Creators: name: Correa-da-Silva, Felipe
name: Kalsbeek, Martin J.
name: Gadella, Femke S.
name: Oppersma, Jorn
name: Jiang, Wei
name: Wolff, Samantha E. C.
name: Korpel, Nikita L.
name: Swaab, Dick F.
name: Fliers, Eric
name: Kalsbeek, Andries
name: Yi, Chun-Xia
Publisher: name: figshare
Year: 2024
Subjects: subject: Cell Biology
subject: Molecular Biology
subject: Neuroscience
subject: Physiology
subject: FOS: Biological sciences
subject: Mental Health

description: Additional file 2. Supplementary information and correalation analysis between neuronal and glial parameters with potential confounders.
Factors affecting refractoriness or recurrence in diffuse large B-cell lymphoma: development and validation of a novel predictive nomogram
Creators: name: Guo, Yiwei
name: Lian, Jie
name: Chen, Yao
name: Quan, Lina
name: Guo, Xiuchen
name: Zhang, Jingbo
name: Liu, Zhiqiang
name: Liu, Aichun
Publisher: name: Taylor & Francis
Year: 2024
Subjects: subject: Medicine
subject: Cell Biology
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Mathematical Sciences not elsewhere classified
subject: Cancer
subject: Hematology
subject: Plant Biology
subject: FOS: Biological sciences

description: Relapsed/Refractory (R/R) diffuse large B-cell lymphoma (DLBCL) represents a subgroup with a high incidence and dismal prognosis. Currently, there is a lack of robust models for predicting R/R DLBCL. Therefore, we conducted a retrospective study to identify key determinants to be incorporated into a novel nomogram to enhance the identification of DLBCL patients at elevated risk of refractoriness/recurrence. We included 293 newly-diagnosed DLBCL patients from Harbin Medical University Cancer Hospital, collected from 2008–2017. Patients were randomly divided into a training cohort (n = 206) and a validation cohort (n = 87) at a 7:3 ratio. The training cohort underwent univariable analysis to select variables for a binary logistic regression model. These variables were also prioritized using a random forest algorithm. The developed nomogram was evaluated with the receiver-operator characteristic (ROC) curve, calibration curve, and decision curve analysis (DCA) for its clinical utility. Univariable analysis pinpointed several factors significantly associated with refractoriness/recurrence, including pathological subtype, lactate dehydrogenase (LDH), International Prognostic Index (IPI), treatment, absolute lymphocyte count (ALC), lymphocyte/monocyte ratio (LMR), and prognostic nutritional index (PNI). Binary logistic regression highlighted pathological subtype, LDH, treatment, and ALC as key predictors, which were incorporated into the nomogram. The nomogram showed excellent calibration and accuracy in both cohorts, and comparative DCA and ROC analysis demonstrated its superior net benefit and area under the curve (AUC) compared to traditional indexes like IPI, R-IPI, and NCCN-IPI. This nomogram serves as a valuable tool for predicting the likelihood of refractoriness or recurrence in DLBCL patients.
Evaluating the impact of a maternity and neonatal emergencies education programme in Australian regional and rural health services on clinician knowledge and confidence: a pre-test post-test study
Creators: name: Cullinane, Meabh
name: Zugna, Stefanie
name: McLachlan, Helen
name: Newton, Michelle
name: Forster, Della
Publisher: name: La Trobe
Year: 2022
Subjects: classificationCode: 420699
subject: Public health not elsewhere classified

description: INTRODUCTION: Almost 78 000 women gave birth in the state of Victoria, Australia, in 2019. While most births occurred in metropolitan Melbourne and large regional centres, a significant proportion of women birthed in rural services. In late 2016, to support clinicians to recognise and respond to clinical deterioration, the Victorian government mandated provision of an emergency training programme, called Maternity and Newborn Emergencies (MANE), to rural and regional maternity services across the state. This paper describes the evaluation of MANE. DESIGN AND SETTING: A quasi-experimental study design was used; the Kirkpatrick Evaluation Model provided the framework. PARTICIPANTS: Participants came from the 17 rural and regional Victorian maternity services who received MANE in 2018 and/or 2019. OUTCOME MEASURES: Baseline data were collected from MANE attendees before MANE delivery, and at four time points up to 12 months post-delivery. Clinicians' knowledge of the MANE learning objectives, and confidence ratings regarding the emergencies covered in MANE were evaluated. The Safety Attitudes Questionnaire (SAQ) assessed safety climate pre-MANE and 6 months post-MANE among all maternity providers at the sites. RESULTS: Immediately post-MANE, most attendees reported increased confidence to escalate clinical concerns (n=251/259). Knowledge in the non-technical and practical aspects of the programme increased. Management of perinatal emergencies was viewed as equally stressful pre-MANE and post-MANE, but confidence to manage these emergencies increased post-delivery. Pre-MANE SAQ scores showed consistently strong and poor performing services. Six months post-MANE, some services showed improvements in SAQ scores indicative of improved safety climate. CONCLUSION: MANE delivery resulted in both short-term and sustained improvements in knowledge of, and confidence in, maternity emergencies. Further investigation of the SAQ across Victoria may facilitate identification of services with a poor safety climate who could benefit from frequent targeted interventions (such as the MANE programme) at these sites.
Skinner's Scientific Spectrum: Issue 3 - June 2024
Creators: name: Skinner, Neo
name: Chapman, Oliver
Publisher: name: figshare
Year: 2024
Subjects: classificationCode: 490402
subject: Algebraic and differential geometry
classificationCode: 461307
subject: Quantum computation
classificationCode: 510803
subject: Quantum information, computation and communication
classificationCode: 510805
subject: Quantum technologies
classificationCode: 340202
subject: Crystallography
classificationCode: 340605
subject: Molecular imaging (incl. electron microscopy and neutron diffraction)
classificationCode: 310803
subject: Plant cell and molecular biology
classificationCode: 310806
subject: Plant physiology
classificationCode: 310802
subject: Plant biochemistry
classificationCode: 310303
subject: Ecological physiology

description: Skinner's Scientific Spectrum is a monthly newsletter published for STEM educators and students. In this issue, articles include: A Five-Sided Square: Ignoring Euclid; From 0 to... 1; Growing Crystals For... Analysis?; and WHEN DINNER SAYS NO!.
Supplemental Material, DS_10.1177_2325967120959303 - Upper Extremity Pain and Overuse Injuries in Fly-Fishing: A North American Cross-Sectional Survey and Implications for Injury Prevention
Creators: name: Kuhn, Andrew W.
name: Kuhn, John E.
Publisher: name: SAGE Journals
Year: 2020
Subjects: subject: 110314 Orthopaedics
subject: FOS: Clinical medicine
subject: 110604 Sports Medicine
subject: FOS: Health sciences

description: Supplemental Material, DS_10.1177_2325967120959303 for Upper Extremity Pain and Overuse Injuries in Fly-Fishing: A North American Cross-Sectional Survey and Implications for Injury Prevention by Andrew W. Kuhn and John E. Kuhn in Orthopaedic Journal of Sports Medicine