title: ÐÐ±ÑÐ°Ð±Ð¾ÑÐºÐ° ÑÐ¸Ð³Ð½Ð°Ð»Ð¾Ð² ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸ Ð¿ÑÐ¸ ÑÐµÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð°Ð½Ð°Ð»Ð¸Ð·Ð¾Ð² Ð² Ð¼Ð¸ÐºÑÐ¾ÑÐ»ÑÐ¸Ð´Ð½ÑÑ ÑÐ¸ÑÑÐµÐ¼Ð°Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Processing of fluorescence signals in the implementation of genetic analysis in microfluid systems
Creators: name: ÐÐ»Ð¸Ð¼ÐµÐ½ÐºÐ¾, ÐÐ°ÑÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: ÐÐ¦Ð -Ð Ð
ÐÐÐ
Ð¿Ð»Ð°Ð²Ð»ÐµÐ½Ð¸Ðµ ÐÐÐ
Ð°Ð¿Ð¿ÑÐ¾ÐºÑÐ¸Ð¼Ð°ÑÐ¸Ñ
Ð¼Ð¸ÐºÑÐ¾ÑÐ»ÑÐ¸Ð´Ð¸ÐºÐ°
Ð¼Ð¸ÐºÑÐ¾ÑÐ»ÑÐ¸Ð´Ð½ÑÐ¹ ÑÐ¸Ð¿
Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÐ° ÑÐ¸Ð³Ð½Ð°Ð»Ð¾Ð²
Ð¿ÑÐ·ÑÑÐ¸
ÑÐ¸Ð³Ð¼Ð¾Ð¸Ð´Ð°Ð»ÑÐ½Ð°Ñ ÑÑÐ½ÐºÑÐ¸Ñ
PCR-RT
DNA
DNA melting
approximation
microfluidics
microfluidicÂ chip
signal processing
bubbles
sigmoid function

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description: One of the main problems in using microfluidic chips is bubbles in it, which movement and formation leads to disorder in the measurement sequence in the form of a distortion of the approximating dependence. Two new methods for automatic processing of distorted qPCR signals were developed and implemented, based on the approximation of qPCR graphs by a sigmoid function. By utilizing these techniques, researchers can achieve an acceptable RMS value when determining the threshold cycle and successfully conduct a quantitative analysis of qPCR in microfluidic chips, even when experiencing bubbles. A method for DNA melting curves processing based on the Gompertz function was proposed. A comparison with the well-known technique based on the sigmoid function was made. The minimum discrepancy between the results was revealed. The sigmoid function method was chosen as the most convenient.