Additional file 1 of High complement protein C1q levels in pulmonary fibrosis and non-small cell lung cancer associated with poor prognosis
Creators: name: Kou, Wenxin
name: Li, Bo
name: Shi, Yeifei
name: Zhao, Yifan
name: Yu, Qing
name: Zhuang, Jianhui
name: Xu, Yawei
name: Peng, Wenhui
Publisher: name: figshare
Year: 2022
Subjects: subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Pharmacology
subject: Biological Sciences not elsewhere classified
subject: Information Systems not elsewhere classified
subject: Mathematical Sciences not elsewhere classified
subject: Cancer
subject: Infectious Diseases
subject: FOS: Health sciences

description: Additional file 1: Supplementary Fig. 1. Venn diagram of GSE37635. Common DEGs at 6 timepoints profiling GSE37635. Supplementary Fig. 2. The heatmap of GSE102751. It indicates the significant differences between the blood of control and IPF patients. The high expression and low expression are represented in red and green, respectively. Supplementary Fig. 3. The heatmap of GSE98468. It indicates the significant differences between the balf of control and IPF mice. The high expression and low expression are represented in red and green, respectively. Supplementary Fig. 4. Immunohistochemistry data of lung tissue. Immunohistochemistry data of C1q (C1qa, C1qb and C1qc) in normal lung, LUAD and LUSC from The Human Protein Atlas. Supplementary Fig. 5. The expression of C1q. C1q (C1qa, C1qb and C1qc) mainly express in macrophages in all cell types and lung tissue from The Human Protein Atlas. Supplementary Fig. 6. The expression of C1q in blood monocytes. The FPKM of C1q in blood monocytes significantly increased in 3-week tumor mice by GSE76033. Supplementary Fig. 7. DNA methylation levels of probes of hub genes in LUAD. (A) 5 methylation probes of C1qa showed significant difference in normal group and LUAD group; (B) 10 methylation probes of C1qb showed significant difference in normal group and LUAD group; (C) 9 methylation probes of C1qc showed significant difference in normal group and LUAD group;(D) 5 methylation probes of Ccr5 showed significant difference in normal group and LUAD group. Supplementary Fig. 8. Methylation levels of hub genes in LUSC. (A) 6 methylation probes of C1qa showed significant difference in normal group and LUSC group; (B) 10 methylation probes of C1qb showed significant difference in normal group and LUAD group; (C) 10 methylation probes of C1qc showed significant difference in normal group and LUSC group;(D) 4 methylation probes of Ccr5 showed significant difference in normal group and LUSC group. Supplementary Fig. 9. Methylation levels of hub genes in IPF. (A) 9 methylation probes of C1qa showed significant difference in normal group and IPF group; (B) 10 methylation probes of C1qb showed significant difference in normal group and IPF group; (C) 9 methylation probes of C1qc showed significant difference in normal group and IPF group;(D) 4 methylation probes of Ccr5 showed significant difference in normal group and IPF group. Supplementary Fig. 10. Overall survival analysis of Ccr5 in NSCLC patients. NSCLC patients with elevated Ccr5 levels had higher OS (p &lt; 0.05). Supplementary Fig. 11. Relationship between Ccr5 and fibrosis in NSCLC. The expression of Ccr5 was positive correlative with α-SMA, COL1A, CTGF and TGF-β1 in NSCLC including LUAD and LUSC at TIMER2.0 database. Supplemental Table 1. Characteristics of the genes included in datasets. Supplemental Table 2. The logFC of DEGs. Up-regulated genes were labeled in red, down-regulated genes were labeled in black. Supplemental Table 3 Characteristics of the genes included in datasets. Supplemental Table 4 Characteristics of human subjects. Supplemental Table 5 The means and standard deviations of FPKM of C1q.