Remodeling of maternal mRNA through poly(A) tail orchestrates human oocyte-to-embryo transitionUntitled Item
Creators: name: al., Liu, Y., Zhao, H., Shao, F. et
Publisher: name: figshare
Year: 2023
Subjects: Cell metabolism
Cell development, proliferation and death

description: 1 of 2282 selected<br> NATURE STRUCTURAL &amp; MOLECULAR BIOLOGY 2023<br> Remodeling of maternal mRNA through poly(A) tail orchestrates human oocyte-to-embryo transition<br> Liu, YushengZhao, HanShao, FanghongZhang, YiweiNie, Hu<br> more <br> Poly(A)-tail-mediated post-transcriptional regulation of maternal mRNAs is vital in the oocyte-to-embryo transition (OET). Nothing is known about poly(A) tail dynamics during the human OET. Here, we show that poly(A) tail length and internal non-A residues are highly dynamic during the human OET, using poly(A)-inclusive RNA isoform sequencing (PAIso-seq). Unexpectedly, maternal mRNAs undergo global remodeling: after deadenylation or partial degradation into 3ʹ-UTRs, they are re-polyadenylated to produce polyadenylated degradation intermediates, coinciding with massive incorporation of non-A residues, particularly internal long consecutive U residues, into the newly synthesized poly(A) tails. Moreover, TUT4 and TUT7 contribute to the incorporation of these U residues, BTG4-mediated deadenylation produces substrates for maternal mRNA re-polyadenylation, and TENT4A and TENT4B incorporate internal G residues. The maternal mRNA remodeling is further confirmed using PAIso-seq2. Importantly, maternal mRNA remodeling is essential for the first cleavage of human embryos. Together, these findings broaden our understanding of the post-transcriptional regulation of maternal mRNAs during the human OET. Comprehensive poly(A)-inclusive RNA isoform sequencing throughout the human oocyte and preimplantation embryo development reveals poly(A)-tail-mediated maternal mRNA remodeling that is essential for human embryo development.1 of 2282 selected 2 <strong>NATURE STRUCTURAL &amp; MOLECULAR BIOLOGY 2023</strong> <strong>Remodeling of maternal mRNA through poly(A) tail orchestrates human oocyte-to-embryo transition</strong> Liu, Yusheng Zhao, Han Shao, Fanghong Zhang, Yiwei Nie, Hu more Poly(A)-tail-mediated post-transcriptional regulation of maternal mRNAs is vital in the oocyte-to-embryo transition (OET). Nothing is known about poly(A) tail dynamics during the human OET. Here, we show that poly(A) tail length and internal non-A residues are highly dynamic during the human OET, using poly(A)-inclusive RNA isoform sequencing (PAIso-seq). Unexpectedly, maternal mRNAs undergo global remodeling: after deadenylation or partial degradation into 3ʹ-UTRs, they are re-polyadenylated to produce polyadenylated degradation intermediates, coinciding with massive incorporation of non-A residues, particularly internal long consecutive U residues, into the newly synthesized poly(A) tails. Moreover, TUT4 and TUT7 contribute to the incorporation of these U residues, BTG4-mediated deadenylation produces substrates for maternal mRNA re-polyadenylation, and TENT4A and TENT4B incorporate internal G residues. The maternal mRNA remodeling is further confirmed using PAIso-seq2. Importantly, maternal mRNA remodeling is essential for the first cleavage of human embryos. Together, these findings broaden our understanding of the post-transcriptional regulation of maternal mRNAs during the human OET. Comprehensive poly(A)-inclusive RNA isoform sequencing throughout the human oocyte and preimplantation embryo development reveals poly(A)-tail-mediated maternal mRNA remodeling that is essential for human embryo development. <br>