title: ÐÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ðµ Ð´ÐµÐ»ÐµÑÐ¸Ð¾Ð½Ð½Ð¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° ÑÐµÐ»Ð¾Ð²ÐµÑÐµÑÐºÐ¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° NUP58 Ð´Ð»Ñ Ð²ÑÑÐ²Ð»ÐµÐ½Ð¸Ñ Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð¾Ð³ÐµÐ½Ð½ÑÑ ÑÑÐ°ÑÑÐºÐ¾Ð²
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Deletion analysis of the human protein NUP58 for amyloidogenesis sites definition
Creators: name: ÐÐ½ÑÐ¾Ð½Ð¾Ð²Ð°, ÐÐºÐ°ÑÐµÑÐ¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Ð½ÑÐºÐ»ÐµÐ¾Ð¿Ð¾ÑÐ¸Ð½
Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´
NUP58
ArchCandy
C-DAG
FG-Ð¿Ð¾Ð²ÑÐ¾ÑÑ
ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑ ÑÐ´ÐµÑÐ½Ð¾Ð¹ Ð¿Ð¾ÑÑ
nucleoporins
amyloids
FG-repeats
nuclear pore complex

description: Ð¦ÐµÐ»Ñ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ â Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð´ÐµÐ»ÐµÑÐ¸Ð¾Ð½Ð½Ð¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð²ÑÑÐ²Ð¸ÑÑ ÑÑÐ°ÑÑÐºÐ¸ ÑÐµÐ»Ð¾Ð²ÐµÑÐµÑÐºÐ¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° NUP58, Ð¾ÑÐ²ÐµÑÐ°ÑÑÐ¸Ðµ Ð·Ð° Ð°Ð³ÑÐµÐ³Ð°ÑÐ¸Ñ. Ð ÑÐ¾Ð´Ðµ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð¿ÑÐ¾ÑÐ¸Ð»Ñ Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð¾Ð³ÐµÐ½Ð½Ð¾ÑÑÐ¸ Ð±ÐµÐ»ÐºÐ° NUP58, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½Ð¾Ð³Ð¾ Ð² Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ðµ ArchCandy, Ð±ÑÐ»Ð¸ Ð²ÑÐ±ÑÐ°Ð½Ñ ÑÐµÑÑÑÐµ ÑÑÐ°Ð³Ð¼ÐµÐ½ÑÐ° Ð´Ð»Ñ Ð¿ÑÐ¾Ð²ÐµÑÐºÐ¸ Ð½Ð° Ð°Ð³ÑÐµÐ³Ð°ÑÐ¸Ñ: NUP58-215-end, NUP58-468-end, NUP58-1-95 Ð¸ NUP58-175-213. ÐÑÐ»Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ñ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ð¸ Ñ Ð´ÐµÐ»ÐµÑÐ¸Ð¾Ð½Ð½ÑÐ¼Ð¸ Ð²Ð°ÑÐ¸Ð°Ð½ÑÐ°Ð¼Ð¸ NUP58, Ð° Ð¸Ð¼ÐµÐ½Ð½Ð¾ Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð´Ñ Ð´Ð»Ñ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð°Ð³ÑÐµÐ³Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð² Ð´ÑÐ¾Ð¶Ð¶ÐµÐ²Ð¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ðµ Ð¸ Ð´Ð»Ñ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð°Ð³ÑÐµÐ³Ð°ÑÐ¸Ð¸ Ð² ÑÐ¸ÑÑÐµÐ¼Ðµ Ð¡-DAG. Ð¢Ð°ÐºÐ¶Ðµ Ð´Ð»Ñ Ð´Ð²ÑÑ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ð¹ NUP58-215-end Ð¸ NUP58-1-95 Ð±ÑÐ»Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ñ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð¿ÑÐ¾Ð²ÐµÑÐºÐ¸ Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð½ÑÑ ÑÐ²Ð¾Ð¹ÑÑÐ²: Ð±ÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ ÑÑÐ°Ð³Ð¼ÐµÐ½Ñ NUP58-1-95 Ð¿ÑÐ¾ÑÐ²Ð»ÑÐµÑ Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð½ÑÐµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° Ð² ÑÐ¸ÑÑÐµÐ¼Ðµ Ð¡-DAG; ÑÑÐ°Ð³Ð¼ÐµÐ½Ñ NUP58_215-end Ð½Ðµ Ð¿ÑÐ¾ÑÐ²Ð»ÑÐµÑ Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð½ÑÐµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° Ð² ÑÐ¸ÑÑÐµÐ¼Ðµ Ð¡-DAG; Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð° Ð°Ð³ÑÐµÐ³Ð°ÑÐ¸Ñ Ð´Ð»Ñ ÑÑÐ°Ð³Ð¼ÐµÐ½ÑÐ¾Ð² NUP58-1-95 Ð¸ NUP58-215-end Ð² Ð´ÑÐ¾Ð¶Ð¶ÐµÐ²Ð¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ðµ Ð½Ð° ÑÐ½Ð¸Ð¼ÐºÐ°Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð³Ð¾ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð°. ÐÐ¾Ð¿Ð¾Ð»Ð½Ð¸ÑÐµÐ»ÑÐ½Ð¾ Ð±ÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾ Ð¿Ð¾Ð»Ð¾Ð¶Ð¸ÑÐµÐ»ÑÐ½Ð¾Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ [PIN+] ÑÑÐ°ÑÑÑÐ° Ð½Ð° Ð°Ð³ÑÐµÐ³Ð°ÑÐ¸Ñ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ð¸ Nup58_215-end, (ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ðµ ÑÐ¸ÑÐ»Ð° Ð°Ð³ÑÐµÐ³Ð°ÑÐ¾Ð²) Ð¸ Ð¾ÑÑÐ¸ÑÐ°ÑÐµÐ»ÑÐ½Ð¾Ðµ Ð½Ð° ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ñ Nup58_1-95, (ÑÐ¼ÐµÐ½ÑÑÐµÐ½Ð¸Ðµ ÑÐ¸ÑÐ»Ð° Ð°Ð³ÑÐµÐ³Ð°ÑÐ¾Ð²). ÐÐ±Ð»Ð°ÑÑÑ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð² Ð²ÐºÐ»ÑÑÐ°ÐµÑ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ðµ ÑÑÐ½Ð´Ð°Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð½Ð°ÑÐºÐ¸ Ð¸ ÑÐµÑÐ°Ð¿Ð¸Ð¸ Ð½ÐµÐ¹ÑÐ¾Ð´ÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸Ð²Ð½ÑÑ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ð¹.
description: The aim of this work is to identify regions of the human NUP58 protein which are responsible for aggregation using deletion analysis. During the analysis of the NUP58 amyloidogenicity profile four fragments were selected for aggregation testing: NUP58-215-end, NUP58-468-end, NUP58-1-95, and NUP58-175-213. Constructions with deletion variants of NUP58 protein were obtained, especially plasmids for the analysis of aggregation in the yeasts and for the analysis of aggregation in the C-DAG system. Also, for the two constructions NUP58-215-end and NUP58-1-95, the following amyloid properties were obtained: the NUP58-1-95 fragment show amyloid properties in the C-DAG system; the NUP58_215-end fragment doesnât show amyloid properties in the C-DAG system; it was shown aggregation for NUP58-1-95 and NUP58-215-end fragments in the yeasts on fluorescence microscope images. Additionally, it was shown a positive effect of [PIN+] status on the aggregation of the Nup58_215-end, construction (an increase in the number of aggregates) and a negative effect on the Nup58_1-95, construction (a decrease in the number of aggregates). The scope of the obtained results includes the development of fundamental science and therapy for neurodegenerative diseases.