title: ÐÐ»ÑÑ-ÐºÐ¾Ð½ÑÐµÐ²Ð¾Ð¹ Ð±ÐµÐ»Ð¾Ðº EB3 ÑÐµÐ³ÑÐ»Ð¸ÑÑÐµÑ ÑÑÑÐ¾ÐµÐ½Ð¸Ðµ Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½Ð¾Ð³Ð¾ Ð´ÐµÑÐµÐ²Ð° Ð¸ Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ñ ÐºÐ°Ð»ÑÐ¼Ð¾Ð´ÑÐ»Ð¸Ð½-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾Ð¹ Ð¿ÑÐ¾ÑÐµÐ¸Ð½ÐºÐ¸Ð½Ð°Ð·Ñ II Ð² Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð°Ð»ÑÐ½ÑÑ Ð½ÐµÐ¹ÑÐ¾Ð½Ð°Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: The plus-terminal protein EB3 regulates the structure of the dendritic tree and the localization of calmodulin-dependent protein kinase II in hippocampal neurons
Creators: name: Ð§Ð¸Ð³ÑÑÐ¹, ÐÐ°ÑÐ³Ð°ÑÐ¸ÑÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: Ð±Ð¾Ð»ÐµÐ·Ð½Ñ ÐÐ»ÑÑÐ³ÐµÐ¸ÌÐ¼ÐµÑÐ°
Ð½ÐµÐ¸ÌÑÐ¾Ð´ÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸Ñ
Ð´Ð¸Ð½Ð°Ð¼Ð¸ÑÐµÑÐºÐ¸Ðµ Ð¼Ð¸ÐºÑÐ¾ÑÑÑÐ±Ð¾ÑÐºÐ¸
Ð³ÑÐ¸Ð±Ð¾Ð²Ð¸Ð´Ð½ÑÐµ ÑÐ¸Ð¿Ð¸ÐºÐ¸
Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½Ð¾Ðµ Ð´ÐµÑÐµÐ²Ð¾
Ð°ÑÐ±Ð¾ÑÐ¸Ð·Ð°ÑÐ¸Ñ
Ð°Ð½Ð°Ð»Ð¸Ð· Ð¨Ð¾Ð»Ð»Ñ
EB3
CaMKII
Alzheimerâs disease
neurodegeneration
dynamic microtubules
mushroom spines
dendritic tree
arborization
Sholl analysis

description: ÐÐµÐ»Ð¾Ðº EB3, ÐºÑÐµÐ¿ÑÑÐ¸Ð¸ÌÑÑ Ðº Ð¿Ð¾Ð»Ð¾Ð¶Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð¼Ñ ÐºÐ¾Ð½ÑÑ ÑÐ°ÑÑÑÑÐ¸Ñ ÑÑÐ±ÑÐ»Ð¸Ð½Ð¾Ð²ÑÑ Ð¼Ð¸ÐºÑÐ¾ÑÑÑÐ±Ð¾ÑÐµÐº, ÑÐµÐ³ÑÐ»Ð¸ÑÑÐµÑ Ð¸Ñ Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÑ Ð¸ Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÐµÑ Ð½ÐµÐ¸ÌÑÐ¾Ð¿ÑÐ¾ÑÐµÐºÑÐ¾ÑÐ½ÑÐ¸Ì Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð» Ð½Ð° Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÐµ ÑÐ¸Ð¿Ð¸ÐºÐ¸ Ð½ÐµÐ¸ÌÑÐ¾Ð½Ð¾Ð² Ð² ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¸Ì Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ ÐÐ»ÑÑÐ³ÐµÐ¸ÌÐ¼ÐµÑÐ°. Ð ÑÐ°Ð±Ð¾ÑÐµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ ÑÑÐ¾Ð²Ð½Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð¿Ð»ÑÑ-ÐºÐ¾Ð½ÑÐµÐ²Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° EB3 Ð½Ð° Ð°ÑÐ±Ð¾ÑÐ¸Ð·Ð°ÑÐ¸Ñ Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ¾Ð² Ð¸ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ ÐºÐ°Ð»ÑÑÐ¸Ð¸Ì/ÐºÐ°Ð»ÑÐ¼Ð¾Ð´ÑÐ»Ð¸Ð½- Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾Ð¸Ì Ð¿ÑÐ¾ÑÐµÐ¸Ð½ÐºÐ¸Ð½Ð°Ð·Ñ Ð²ÑÐ¾ÑÐ¾Ð³Ð¾ ÑÐ¸Ð¿Ð° Ð² Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ°Ñ Ð¸ Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÑ ÑÐ¸Ð¿Ð¸ÐºÐ°Ñ Ð¿ÐµÑÐ²Ð¸ÑÐ½ÑÑ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð°Ð»ÑÐ½ÑÑ Ð½ÐµÐ¸ÌÑÐ¾Ð½Ð¾Ð². ÐÐ¾ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ°Ð¼ Ð´Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¾, ÑÑÐ¾ EB3 Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÐµÑ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð½Ð° Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ñ Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½Ð¾Ð³Ð¾ Ð´ÐµÑÐµÐ²Ð°: Ð¿ÑÐ¸ Ð½Ð¾ÐºÐ°ÑÑÐµ Ð¸ Ð³Ð¸Ð¿ÐµÑÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð´Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° Ð½Ð° 7 Ð´ÐµÐ½Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ in vitro Ð¿ÑÐ¾Ð¸ÑÑÐ¾Ð´Ð¸Ñ Ð·Ð½Ð°ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ðµ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ðµ Ð¾Ð±ÑÐµÐ¸Ì Ð´Ð»Ð¸Ð½Ñ Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ¾Ð² Ð¸ Ð¸Ñ Ð²ÐµÑÐ²Ð»ÐµÐ½Ð¸Ñ. Ð ÑÐ°Ð±Ð¾ÑÐµ Ð¿ÑÐ¾Ð¸Ð·Ð²ÐµÐ´ÐµÐ½Ð° Ð¾ÑÐµÐ½ÐºÐ° ÑÐ¾Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ñ ÑÑÐ¾Ð²Ð½Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ ÑÐ¸Ð½Ð°Ð¿Ñ- ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐ½Ð¾Ð¸Ì ÐºÐ¸Ð½Ð°Ð·Ñ CaMKII Ð² Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ¾Ð¼ ÑÐ¸Ð¿Ð¸ÐºÐµ Ðº Ð´ÐµÐ½Ð´ÑÐ¸ÑÑ. ÐÐ¸Ð¿ÐµÑÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ EB3 ÑÑÐ¸Ð¼ÑÐ»Ð¸ÑÑÐµÑ Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ñ CaMKII Ð² Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÑ ÑÐ¸Ð¿Ð¸ÐºÐ°Ñ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð°Ð»ÑÐ½ÑÑ Ð½ÐµÐ¸ÌÑÐ¾Ð½Ð¾Ð² Ð¿Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ Ñ Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ°Ð¼Ð¸ Ð¸ Ð¿ÑÐµÐ´Ð¾ÑÐ²ÑÐ°ÑÐ°ÐµÑ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ðµ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð´Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° Ð² Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÑ ÑÐ¸Ð¿Ð¸ÐºÐ°Ñ Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ Ð½Ð¸Ð·ÐºÐ¾Ð¸Ì Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð½Ð¾Ð¸Ì ÑÐ¾ÐºÑÐ¸ÑÐ½Ð¾ÑÑÐ¸. Ð¢Ð°ÐºÐ¸Ð¼ Ð¾Ð±ÑÐ°Ð·Ð¾Ð¼, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ ÑÐºÐ°Ð·ÑÐ²Ð°ÑÑ Ð½Ð° Ð²Ð°Ð¶Ð½Ð¾ÑÑÑ EB3 Ð² ÑÐµÐ³ÑÐ»ÑÑÐ¸Ð¸ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð°Ð»ÑÐ½ÑÑ Ð½ÐµÐ¸ÌÑÐ¾Ð½Ð¾Ð² Ð¸ ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ ÑÐ¸Ð½Ð°Ð¿ÑÐ¾Ð².
description: EB3 protein, attached to the end of growing tubulin microtubules regulates their dynamics and exerts neuroprotective potential on dendritic spines of neurons in a cellular model of Alzheimer's disease. In the following study we have investigated the expression level effect of the plus-end tracking proteins EB3 on dendritic arborization and the expression of calcium/calmodulin-dependent protein kinase II in dendrites and dendritic spines of primary hippocampal neurons. According to the results of this study, it was defined that EB3 affects the dendritic tree morphology: with knockout and overexpression of this protein on the 7th day of in vitro cultivation, there is a significant decrease in dendrites total length and their branching. The work evaluates the ratio of the expression level of the synapse- specific kinase CaMKII in the dendritic spine to the dendrite. EB3 overexpression stimulates CaMKII localization in dendritic spines of hippocampal neurons compared to dendrites and prevents a decrease in protein expression in dendritic spines under conditions of low amyloid toxicity. Thus, the significance of EB3 in the regulation of the morphology of hippocampal neurons and the function of synapses is indicated.