title: ÐÐ·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Ð¼ÐµÑÐ°Ð±Ð¾ÑÑÐ¾Ð¿Ð½ÑÑ ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð² Ð³Ð»ÑÑÐ°Ð¼Ð°ÑÐ° Ð² ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ ÑÑÑÑÐºÑÑÑÐ°Ñ Ð¼Ð¾Ð·Ð³Ð° ÐºÑÑÑ Ð² Ð»Ð¸ÑÐ¸Ð¹-Ð¿Ð¸Ð»Ð¾ÐºÐ°ÑÐ¿Ð¸Ð½Ð¾Ð²Ð¾Ð¹ Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð²Ð¸ÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Changes in gene expression of metabotropic glutamate receptors in different rat brain structures in a lithium-pilocarpine model of temporal lobe epilepsy
Creators: name: Ð§ÐµÑÐ½Ð¸Ð³Ð¸Ð½Ð°, ÐÐ¾Ð»Ð¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ñ
Ð»Ð¸ÑÐ¸Ð¹-Ð¿Ð¸Ð»Ð¾ÐºÐ°ÑÐ¿Ð¸Ð½Ð¾Ð²Ð°Ñ Ð¼Ð¾Ð´ÐµÐ»Ñ
Ð²Ð¸ÑÐ¾ÑÐ½Ð°Ñ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ñ
Ð¼ÐµÑÐ°Ð±Ð¾ÑÑÐ¾Ð½ÑÐµ ÑÐµÑÐµÐ¿ÑÐ¾ÑÑ Ð³Ð»ÑÑÐ°Ð¼Ð°ÑÐ°
ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¾Ð³ÐµÐ½ÐµÐ·
Ð³Ð»ÑÑÐ°Ð¼Ð°Ñ
Ð¾Ñ-Ð¿ÑÑ
epilepsy
lithium-pylocarpine model
temporal lobe epilepsy
metabotropic glutamate receptors
epileptogenesis
glutamate
RT-qPCR

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«ÐÐ·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Ð¼ÐµÑÐ°Ð±Ð¾ÑÑÐ¾Ð¿Ð½ÑÑ ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð² Ð³Ð»ÑÑÐ°Ð¼Ð°ÑÐ° Ð² ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ ÑÑÑÑÐºÑÑÑÐ°Ñ Ð¼Ð¾Ð·Ð³Ð° ÐºÑÑÑ Ð² Ð»Ð¸ÑÐ¸Ð¹-Ð¿Ð¸Ð»Ð¾ÐºÐ°ÑÐ¿Ð¸Ð½Ð¾Ð²Ð¾Ð¹ Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð²Ð¸ÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ð¸Â». ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Ð¼ÐµÑÐ°Ð±Ð¾ÑÑÐ¾Ð¿Ð½ÑÑ ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð² Ð³Ð»ÑÑÐ°Ð¼Ð°ÑÐ° I, II Ð¸ III Ð³ÑÑÐ¿Ð¿Ñ (mGluRs) Ð² Ð»Ð¸ÑÐ¸Ð¹-Ð¿Ð¸Ð»Ð¾ÐºÐ°ÑÐ¿Ð¸Ð½Ð¾Ð²Ð¾Ð¹ Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð²Ð¸ÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ð¸. Ð Ð°Ð±Ð¾ÑÐ° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ ÐÐ°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½ÑÑ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð¾Ð² Ð½ÐµÐ¹ÑÐ¾Ð½Ð½ÑÑ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ð¹ ÐÐ­Ð¤Ð Ð ÐÐ. ÐÐ½Ð°Ð»Ð¸Ð· ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Grm1, Grm2, Grm3, Grm4, Grm5, Grm7, Grm8 Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»Ð¸ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ ÐÐ¢-ÐÐ¦Ð  Ð² ÑÐµÐ°Ð»ÑÐ½Ð¾Ð¼ Ð²ÑÐµÐ¼ÐµÐ½Ð¸ Ð² Ð´Ð¾ÑÐ·Ð°Ð»ÑÐ½Ð¾Ð¹ Ð¸ Ð²ÐµÐ½ÑÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð¾Ð±Ð»Ð°ÑÑÑÑ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð°, Ð° ÑÐ°ÐºÐ¶Ðµ Ð²Ð¸ÑÐ¾ÑÐ½Ð¾Ð¹ ÐºÐ¾ÑÐµ ÐºÑÑÑ, Ð¿ÐµÑÐµÐ½ÐµÑÑÐ¸Ñ Ð»Ð¸ÑÐ¸Ð¹-Ð¿Ð¸Ð»Ð¾ÐºÐ°ÑÐ¿Ð¸Ð½-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐµ ÑÑÐ´Ð¾ÑÐ¾Ð³Ð¸. Ð¡ÑÐ´Ð¾ÑÐ¾Ð³Ð¸, Ð¸Ð½Ð´ÑÑÐ¸ÑÑÐµÐ¼ÑÐµ Ð¿Ð¸Ð»Ð¾ÐºÐ°ÑÐ¿Ð¸Ð½Ð¾Ð¼, Ð¿ÑÐ¸Ð²Ð¾Ð´Ð¸Ð»Ð¸ Ðº Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸ÑÐ¼ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Ð¼ÐµÑÐ°Ð±Ð¾ÑÑÐ¾Ð¿Ð½ÑÑ ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð² Ð³Ð»ÑÑÐ°Ð¼Ð°ÑÐ° Ð²Ð¾ Ð²ÑÐµÑ Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼ÑÑ ÑÑÑÑÐºÑÑÑÐ°Ñ Ð¼Ð¾Ð·Ð³Ð°. ÐÑÐ»Ð¾ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¾ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ðµ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² mGluRs II Ð¸ III Ð³ÑÑÐ¿Ð¿ Ð² Ð²Ð¸ÑÐ¾ÑÐ½Ð¾Ð¹ ÐºÐ¾ÑÐµ, Grm7 Ð¸ Grm8 III Ð³ÑÑÐ¿Ð¿Ñ Ð² Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ðµ Ð¸ Ð½Ð°Ð¿ÑÐ¾ÑÐ¸Ð², ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ðµ Ð¿ÑÐ¾Ð´ÑÐºÑÐ¸Ð¸ Ð¼Ð ÐÐ Grm5 Ð² Ð»Ð°ÑÐµÐ½ÑÐ½ÑÑ ÑÐ°Ð·Ñ Ð¼Ð¾Ð´ÐµÐ»Ð¸. Ð¥Ð°ÑÐ°ÐºÑÐµÑ Ð²ÑÑÐ²Ð»ÐµÐ½Ð½ÑÑ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹ Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÐµÑ Ð¿ÑÐµÐ´Ð¿Ð¾Ð»Ð¾Ð¶Ð¸ÑÑ, ÑÑÐ¾ Ð¾Ð½Ð¸ Ð¼Ð¾Ð³ÑÑ Ð²Ð½Ð¾ÑÐ¸ÑÑ ÑÑÑÐµÑÑÐ²ÐµÐ½Ð½ÑÐ¹ Ð²ÐºÐ»Ð°Ð´ Ð² Ð¿ÑÐ¾ÑÐµÑÑÑ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ð·Ð°ÑÐ¸Ð¸ Ð¼Ð¾Ð·Ð³Ð°. Ð¢Ð°ÐºÐ¸Ð¼ Ð¾Ð±ÑÐ°Ð·Ð¾Ð¼, Ð´Ð°Ð½Ð½Ð¾Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ ÑÐ³Ð»ÑÐ±Ð»ÑÐµÑ Ð½Ð°ÑÐ¸ Ð·Ð½Ð°Ð½Ð¸Ñ Ð¾Ð± ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² mGluRs Ð½Ð° ÑÐ°Ð·Ð½ÑÑ ÑÑÐ°Ð´Ð¸ÑÑ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¾Ð³ÐµÐ½ÐµÐ·Ð°. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð¼Ð¾Ð³ÑÑ Ð±ÑÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ Ð´Ð»Ñ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ¸ Ð¿ÑÐµÐ²ÐµÐ½ÑÐ¸Ð²Ð½Ð¾Ð¹ ÑÐµÑÐ°Ð¿Ð¸Ð¸ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ð¸.
description: The subject of the graduate qualification work is âChanges in gene expres-sion of metabotropic glutamate receptors in different rat brain structures in a lith-ium-pilocarpine model of temporal lobe epilepsyâ. The work is devoted to the study of changes in the expression of group I, II, and III metabotropic glutamate receptor (mGluRs) genes in a lithium-pilocarpine model of temporal lobe epilepsy. This work was performed in the Laboratory of Molecular Mechanisms of Neuronal Interactions of the IEFB RAS. Analysis of Grm1, Grm2, Grm3, Grm4, Grm5, Grm7, Grm8 gene expression was performed by real-time RT-qPCR in the dorsal and ventral regions of hippo-campus, and temporal cortex of rats after lithium-pilocarpine-induced seizures. Pilocarpine-induced seizures led to changes in the expression of metabotropic glutamate receptor genes in all studied brain structures. A decrease in the gene ex-pression of mGluRs group II and III in the temporal cortex, Grm7 and Grm8 (group III) in the hippocampus, and on the contrary, an increase in Grm5 (group I) mRNA production during the latent phase of the model were found. The detected changes suggest that they may contribute to the epileptogenesis. Thus, this study advances our knowledge of mGluRs gene expression at different stages of epileptogenesis. The findings can be used to develop preventive therapy for epilepsy.