title: Ð¡Ð¾Ð²ÐµÑÑÐµÐ½ÑÑÐ²Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¼ÐµÑÐ¾Ð´Ð° ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²ÐµÐ½Ð½Ð¾Ð¹ Ð¾ÑÐµÐ½ÐºÐ¸ Ð¼Ð¸ÐºÑÐ¾Ð ÐÐ-371Ð° Ð² Ð¿Ð»Ð°Ð·Ð¼Ðµ Ñ Ð¿Ð°ÑÐ¸ÐµÐ½ÑÐ¾Ð² Ñ Ð¾Ð½ÐºÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ð¼Ð¸ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸ÑÐ¼Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Improving the method of quantitative assessment of microRNA-371a in plasma in patients with cancer
Creators: name: ÐÐ¸ÑÐ¾Ð½ÑÑÐµÐ²Ð°, ÐÑÐµÐ½Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð¼Ð¸ÐºÑÐ¾Ð ÐÐ
Ð³ÐµÑÐ¼Ð¸Ð½Ð¾Ð³ÐµÐ½Ð½ÑÐµ Ð¾Ð¿ÑÑ Ð¾Ð»Ð¸
Ð¾Ð¿ÑÑ Ð¾Ð»Ð¸ ÑÐ¸ÑÐµÐº
Ð¾Ð±ÑÐ°ÑÐ½Ð°Ñ ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸Ñ Ñ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐµÐ¹ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð½Ð¾Ð¹ ÑÐµÐ¿Ð½Ð¾Ð¹ ÑÐµÐ°ÐºÑÐ¸ÐµÐ¹ (ÐÐ¢-ÐÐ¦Ð )
microRNAs
germ cell tumors
testicular tumors
reverse transcriptase followed by polymerase chain reaction (RT-PCR)

description: Ð¦ÐµÐ»Ñ Ð´Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: ÑÐ¾Ð²ÐµÑÑÐµÐ½ÑÑÐ²Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¼ÐµÑÐ¾Ð´Ð° ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²ÐµÐ½Ð½Ð¾Ð¹ Ð¾ÑÐµÐ½ÐºÐ¸ Ð¼Ð¸ÐºÑÐ¾Ð ÐÐ-371Ð°-3Ñ Ð² Ð¿Ð»Ð°Ð·Ð¼Ðµ Ñ Ð¿Ð°ÑÐ¸ÐµÐ½ÑÐ¾Ð² Ñ Ð³ÐµÑÐ¼Ð¸Ð½Ð¾Ð³ÐµÐ½Ð½ÑÐ¼Ð¸ Ð¾Ð¿ÑÑÐ¾Ð»ÑÐ¼Ð¸ ÑÐ¸ÑÐµÐº. ÐÐ±ÑÐµÐºÑ ÑÐ°Ð±Ð¾ÑÑ: Ð¿Ð»Ð°Ð·Ð¼Ð° ÐºÑÐ¾Ð²Ð¸ Ð¼ÑÐ¶ÑÐ¸Ð½ Ð´Ð²ÑÑ Ð³ÑÑÐ¿Ð¿: Ð¿Ð°ÑÐ¸ÐµÐ½ÑÐ¾Ð² Ñ ÐÐÐ¯, Ð¿ÑÐ¾ÑÐ¾Ð´Ð¸Ð²ÑÐ¸Ñ Ð»ÐµÑÐµÐ½Ð¸Ðµ Ð² Ð¤ÐÐÐ£ "ÐÐÐÐ¦ Ð¾Ð½ÐºÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¸Ð¼ÐµÐ½Ð¸ Ð.Ð. ÐÐµÑÑÐ¾Ð²Ð°" Ð¸ Ð·Ð´Ð¾ÑÐ¾Ð²ÑÑ Ð´Ð¾Ð½Ð¾ÑÐ¾Ð². ÐÑÐµÐ´Ð¼ÐµÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: Ð¼Ð¸ÐºÑÐ¾Ð ÐÐ-371Ð°-3Ñ Ð² Ð¿Ð»Ð°Ð·Ð¼Ðµ Ñ Ð¿Ð°ÑÐ¸ÐµÐ½ÑÐ¾Ð² Ñ Ð³ÐµÑÐ¼Ð¸Ð½Ð¾Ð³ÐµÐ½Ð½ÑÐ¼Ð¸ Ð¾Ð¿ÑÑÐ¾Ð»ÑÐ¼Ð¸ ÑÐ¸ÑÐºÐ°. Ð ÑÐ¾Ð´Ðµ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¾ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÐµÐ½Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ðµ Ð´Ð²ÑÑ Ð½Ð°Ð±Ð¾ÑÐ¾Ð² Ð´Ð»Ñ Ð²Ñ-Ð´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¼Ð¸ÐºÑÐ¾Ð ÐÐ Ð¸Ð· Ð¿Ð»Ð°Ð·Ð¼Ñ ÐºÑÐ¾Ð²Ð¸, ÑÐ¾Ð·Ð´Ð°Ð½Ð° ÑÐ¸ÑÑÐµÐ¼Ð° Ð´Ð»Ñ ÐÐ¢-ÐÐ¦Ð  Ð´Ð»Ñ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²ÐµÐ½Ð½Ð¾Ð¹ Ð¾ÑÐµÐ½ÐºÐ¸ Ð¼Ð¸ÐºÑÐ¾Ð ÐÐ-371Ð°, Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ðµ Ð½Ð¾Ð²Ð¾Ð¹ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½-Ð½Ð¾Ð¹ Ð¸ ÑÐ¶Ðµ ÑÑÑÐµÑÑÐ²ÑÑÑÐµÐ¹ ÑÐ¸ÑÑÐµÐ¼Ñ, Ð±ÑÐ»Ð¸ Ð¿ÑÐ¾Ð°Ð½Ð°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°Ð½Ñ ÑÑÐ¾Ð²Ð½Ð¸ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð¼Ð¾Ð»ÐµÐºÑÐ» Ð¼Ð¸ÐºÑÐ¾Ð ÐÐ-371Ð° Ð² Ð¿Ð»Ð°Ð·Ð¼Ðµ Ð·Ð´Ð¾ÑÐ¾Ð²ÑÑ Ð´Ð¾Ð½Ð¾ÑÐ¾Ð² Ð¸ Ð¿Ð°ÑÐ¸ÐµÐ½ÑÐ¾Ð² Ñ ÐÐÐ¯ Ñ Ð¿Ð¾-Ð¼Ð¾ÑÑÑ ÐÐ¢-ÐÐ¦Ð , Ð¿Ð¾ÑÑÑÐ¾ÐµÐ½Ð° ROC-ÐºÑÐ¸Ð²Ð°Ñ (AUC=0,7) Ð¸ Ð¾ÑÐµÐ½ÐµÐ½Ð° Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾ÑÑÑ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð¼Ð¸ÐºÑÐ¾Ð ÐÐ-371Ð°-3Ñ Ð² Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐµ ÐÐÐ¯ Ñ Ð¼ÑÐ¶ÑÐ¸Ð½. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¾ Ð²ÑÑÐ²Ð»ÐµÐ½Ð¾, ÑÑÐ¾ ÑÑÐ¾Ð²ÐµÑÑÐµÐ½ÑÑÐ²Ð¾Ð²Ð°Ð½Ð½ÑÐ¹ Ð¼Ðµ-ÑÐ¾Ð´ Ð¾Ð±Ð»Ð°Ð´Ð°ÐµÑ Ð½ÐµÐ´Ð¾ÑÑÐ°ÑÐ¾ÑÐ½Ð¾Ð¹ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð·Ð½Ð°ÑÐ¸Ð¼Ð¾ÑÑÑÑ Ð´Ð»Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ñ Ð² ÐºÐ»Ð¸Ð½Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð¿ÑÐ°ÐºÑÐ¸ÐºÐµ. ÐÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼Ñ Ð´Ð¾Ð¿Ð¾Ð»Ð½Ð¸ÑÐµÐ»ÑÐ½ÑÐµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ. ÐÑÐ¸ Ð½Ð°Ð¿Ð¸ÑÐ°Ð½Ð¸Ð¸ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±Ð·Ð¾ÑÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ: PubMed, eLIBRARY.ru. Ð¡ÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÑÑ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»Ð¸ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿Ð°ÐºÐµÑÐ° Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼ Microsoft Excel, Microsoft Word.
description: The purpose of this study: to improve the method for quantitative assessment of microRNA-371a-3p in plasma in patients with testicular germ cell tumors. Object of work: blood plasma of men of two groups: patients with GCT who were treated at the Federal State Budgetary Institution "N.N. Petrov National Medical Research Center of Oncology" and healthy donors. Subject of study: microRNA-371a-3p in plasma in patients with testicular germ cell tumors. In the course of this work, two kits for isolating microRNA from blood plasma were compared, a system for RT-PCR was created for the quantitative assessment of microRNA-371a, a comparison of a new modified and an existing system was carried out, and the expression levels of microRNA molecules were analyzed -371a in the plasma of healthy donors and patients with GCT using RT-PCR, an ROC curve (AUC = 0.7) was constructed and the possibility of using microRNA-371a-3p in the diagnosis of GCT in men was assessed. As a result of the study, it was revealed that the improved method has insufficient diagnostic significance for use in clinical practice. More research is needed. When writing the literature review, the following databases were used: PubMed, eLIBRARY.ru. Statistical processing of experimental data was carried out using the Microsoft Excel and Microsoft Word software packages.