Regulation of lipid metabolism-related gene expression in whole blood cells of normo- and dyslipidemic men after fish oil supplementation
Creators: name: Schmidt, Simone
name: Willers, Janina
name: Stahl, Frank
name: Mutz, Kai-Oliver
name: Scheper, Thomas
name: Hahn, Andreas
name: Schuchardt, Jan Philipp
Publisher: name: London : BioMed Central Ltd.
Year: 2012
Subjects: subject: Dyslipidemia
subject: HNF
subject: Omega-3 fatty acids
subject: PPARα
subject: RXR
subject: TG lowering
subject: 2 acylglycerol o acyltransferase 2
subject: 2 acylglycerol o acyltransferase 3
subject: ABC transporter G5
subject: acyltransferase
subject: apolipoprotein C2
subject: cholesterol acyltransferase
subject: cholesterol acyltransferase 1
subject: diacylglycerol acyltransferase 1
subject: docosahexaenoic acid
subject: fish oil
subject: hepatic nuclear factor 1beta
subject: hepatic nuclear factor 6
subject: high density lipoprotein cholesterol
subject: icosapentaenoic acid
subject: low density lipoprotein receptor
subject: membrane protein
subject: nuclear factor
subject: omega 3 fatty acid
subject: peroxisome proliferator activated receptor alpha
subject: phospholipid transfer protein
subject: retinoid X receptor alpha
subject: retinoid X receptor gamma
subject: triacylglycerol
subject: unclassified drug
subject: article
subject: blood cell
subject: blood sampling
subject: cholesterol metabolism
subject: clinical article
subject: controlled clinical trial
subject: controlled study
subject: diet supplementation
subject: dyslipidemia
subject: gene expression
subject: genome analysis
subject: human
subject: lipid blood level
subject: lipid metabolism
subject: lipogenesis
subject: male
subject: microarray analysis
subject: nucleotide sequence
subject: protein blood level
subject: real time polymerase chain reaction
subject: Adult
subject: Cholesterol, HDL
subject: Dietary Supplements
subject: Dyslipidemias
subject: Fatty Acids, Omega-3
subject: Fish Oils
subject: Gene Expression
subject: Genome, Human
subject: Humans
subject: Lipid Metabolism
subject: Lipids
subject: Lipoproteins, LDL
subject: Male
subject: Microarray Analysis
subject: Middle Aged
subject: PPAR alpha
subject: Triglycerides
subject: Dewey Decimal Classification::500 | Naturwissenschaften
subject: Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie

description: Background: Beneficial effects of omega-3 polyunsaturated fatty acids (n-3 PUFAs) on the lipid levels of dyslipidemic subjects are widely described in the literature. However, the underlying molecular mechanisms are largely unknown. The aim of this study was to investigate the effects of n-3 PUFAs on the expression of lipid metabolism-related genes in normo- and dyslipidemic men to unveil potential genes and pathways affecting lipid metabolism. Methods. Ten normo- and ten dyslipidemic men were supplemented for twelve weeks with six fish oil capsules per day, providing 1.14 g docosahexaenoic acid and 1.56 g eicosapentaenoic acid. The gene expression levels were determined by whole genome microarray analysis and quantitative real-time polymerase chain reaction. Results: Several transcription factors (peroxisome proliferator-activated receptor α (PPARα), retinoid X receptor (RXR) α, RXRγ, hepatic nuclear factor (HNF) 6, and HNF1ß) as well as other genes related to triacylglycerol (TG) synthesis or high-density lipoprotein (HDL-C) and cholesterol metabolism (phospholipids transfer protein, ATP-binding cassette sub-family G member 5, 2-acylglycerol O-acyltransferase (MOGAT) 3, MOGAT2, diacylglycerol O-acyltransferase 1, sterol O-acyltransferase 1, apolipoprotein CII, and low-density lipoprotein receptor) were regulated after n-3 PUFA supplementation, especially in dyslipidemic men. Conclusion: Gene expression analyses revealed several possible molecular pathways by which n-3 PUFAs lower the TG level and increase the HDL-C and low-density lipoprotein level, whereupon the regulation of PPARα appear to play a central role. Trial registration. ClinicalTrials.gov (ID: NCT01089231).