A comparative study on the chemo-enzymatic upgrading of renewable biomass to 5-Hydroxymethylfurfural
Creators: name: Kongkona Saikia
name: Abiram Karanam Rathankumar
name: Ramachandran, Krishnakumar
name: Harshini Sridharan
name: Pranay Bohra
name: Bharadwaj, Nikhil
name: Anisha Vyas
name: Vaidyanathan Vinoth Kumar
Publisher: name: Taylor & Francis
Year: 2020
Subjects: subject: Biophysics
subject: Biochemistry
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: FOS: Chemical sciences
subject: Ecology
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: 69999 Biological Sciences not elsewhere classified

description: 5-hydroxymethylfurfural (HMF) obtained from renewable biomass-derived carbohydrates is a potential sustainable substitute to petroleum-based building blocks. In the present work, we constituted a comparative study on the production of HMF from two widely available real biomasses in India- <i>Agave americana</i> and <i>Casuarina equisetifolia</i>. In the initial hydrolysis studies for the production of reducing sugars, 649.5 mg/g of fructose was obtained from the hydrolysis of 5% (w/v) <i>A. americana</i> biomass by the enzyme inulinase in 3 h at 50°C. Similarly, upon hydrolysis of 15% (w/v) <i>C. equisetifolia</i> biomass by the lignocellulolytic enzymes (laccase, cellulase and xylanase) from <i>Trichoderma atroviride</i>, 456.65 mg/g of reducing sugars was released in 24 h at 30°C. Subsequently, the dehydration of the obtained reducing sugars to HMF was achieved with titanium dioxide as the catalyst. The dehydration of <i>A. americana-</i>derived fructose at 140°C led to a maximum HMF yield of 92.6% in 15 min with 10% catalyst load. Contrarily, upon optimizing the process parameters for dehydration of <i>C. equisetifolia</i> derived reducing sugars, the maximum HMF yield of 85.7% was obtained at 110°C in 25 min with a TiO<sub>2</sub> concentration of 10%. This study reports for the first time the utilization of <i>C. equisetifolia</i> biomass for HMF production and thus, by utilizing these inexpensive, abundantly available and highly functionalized polysaccharides, a strategical approach can be developed for the production of fine chemicals, eliminating the need of fossil-based chemicals. <i>Implications</i>: The catalytic upgrading of lignocellulosic biomass into high-valued platform chemicals like 5-Hydroxymethylfurfural (HMF) implies an extremely significant challenge to the attempts of establishing a green economy. <i>Casuarina equisetifolia</i> and <i>Agave americana</i> represents a sustainable feedstock for the production of HMF through catalytic integration. The present work describes a two-step reaction process where the initial depolymerization step comprises of an enzymatic hydrolysis followed by a chemical-catalyst mediated dehydration process. The utilization of a biocatalytic approach followed by mild chemical catalyst eliminates the need of hazardous chemical conversion processes. Thus, the HMF produced via sustainable can bridge the gap between carbohydrate chemistry and petroleum-based industrial chemistry because of the wide range of chemical intermediates and end-products that can be derived from this compound.