title: Ð ÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸Ð²Ð½ÑÐµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° Ð²ÐµÐ·Ð¸ÐºÑÐ» ÑÐ°ÑÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ Ð¿ÑÐ¾Ð¸ÑÑ Ð¾Ð¶Ð´ÐµÐ½Ð¸Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Regenerative properties of plant extracellular vesicles
Creators: name: Ð¡Ð¾Ð»Ð¾Ð¼Ð¸Ð½Ð°, ÐÐ¸Ð´Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: ÑÐ°ÑÑÐ¸ÑÐµÐ»ÑÐ½ÑÐµ ÑÐºÑÑÑÐ°ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐµ Ð²ÐµÐ·Ð¸ÐºÑÐ»Ñ
ÑÐ¸Ð±ÑÐ¾Ð±Ð»Ð°ÑÑÑ
ÐºÐµÑÐ°ÑÐ¸Ð½Ð¾ÑÐ¸ÑÑ
Ð·Ð°Ð¶Ð¸Ð²Ð»ÐµÐ½Ð¸Ðµ ÑÐ°Ð½
plant extracellular vesicles
fibroblasts
keratinocytes
wound healing

description: Ð Ð°ÑÑÐ¸ÑÐµÐ»ÑÐ½ÑÐµ ÑÐºÑÑÑÐ°ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐµ Ð²ÐµÐ·Ð¸ÐºÑÐ»Ñ (Ð Ð­Ð) ÑÐ²Ð»ÑÑÑÑÑ Ð¿ÐµÑÑÐ¿ÐµÐºÑÐ¸Ð²Ð½ÑÐ¼Ð¸ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ°Ð¼Ð¸ Ð´Ð»Ñ Ð»ÐµÑÐµÐ½Ð¸Ñ Ð¿Ð¾Ð²ÑÐµÐ¶Ð´ÐµÐ½Ð¸Ð¹ ÐºÐ¾Ð¶Ð¸. Ð¦ÐµÐ»ÑÑ Ð´Ð°Ð½Ð½Ð¾Ð¹ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÑÐºÐ¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ ÑÑÐ°Ð»Ð° Ð¾ÑÐµÐ½ÐºÐ° Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð Ð­Ð Ð½Ð° ÑÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸Ñ ÐºÐ»ÐµÑÐ¾Ðº ÐºÐ¾Ð¶Ð¸ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð¿Ð¾ÑÐ»Ðµ Ð¸Ñ Ð¼ÐµÑÐ°Ð½Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¿Ð¾Ð²ÑÐµÐ¶Ð´ÐµÐ½Ð¸Ñ in vitro. Ð Ð­Ð Ð±ÑÐ»Ð¸ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ñ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÐ»ÑÑÑÐ°ÑÐµÐ½ÑÑÐ¸ÑÑÐ³Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¸Ð· Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹, ÑÐ»Ð°Ð¼Ð¸Ð´Ð¾Ð¼Ð¾Ð½Ð°Ð´Ñ (Chlamydomonas reinhardtii CC125) Ð¸ ÑÐ»Ð¾ÑÐµÐ»Ð»Ñ (Parachlorella kessleri IPPAS c15), Ð° ÑÐ°ÐºÐ¶Ðµ Ð¸Ð· Ð¿Ð»Ð¾Ð´Ð¾Ð² ÑÐ¾Ð¼Ð°ÑÐ° Ð¸ Ð¾Ð³ÑÑÑÐ°. ÐÐ»ÐµÑÐºÐ¸ Ð»Ð¸Ð½Ð¸Ð¹ HaCat (ÐºÐµÑÐ°ÑÐ¸Ð½Ð¾ÑÐ¸ÑÑ), DF-2 (ÑÐ¸Ð±ÑÐ¾Ð±Ð»Ð°ÑÑÑ), HEK293T (ÐºÐ»ÐµÑÐºÐ¸ ÑÐ¼Ð±ÑÐ¸Ð¾Ð½Ð°Ð»ÑÐ½Ð¾Ð¹ Ð¿Ð¾ÑÐºÐ¸ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°) Ð² Ð¼Ð¾Ð½Ð¾ÑÐ»Ð¾Ðµ Ð¿Ð¾Ð²ÑÐµÐ¶Ð´Ð°Ð»Ð¸ Ð¸ ÑÐ¾-ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð»Ð¸ Ñ Ð Ð­Ð Ð² ÑÐµÑÐµÐ½Ð¸Ðµ 24 ÑÐ°ÑÐ¾Ð². ÐÐ½Ð°Ð»Ð¸Ð· Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð Ð­Ð Ð½Ð° Ð¿ÑÐ¾Ð»Ð¸ÑÐµÑÐ°ÑÐ¸Ð²Ð½ÑÑ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð½ÐµÐ¿Ð¾Ð²ÑÐµÐ¶Ð´ÐµÐ½Ð½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº (ÑÐ¸ÑÐ¾ÑÐ¾ÐºÑÐ¸ÑÐ½Ð¾ÑÑÑ) Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»Ð¸ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ RTCA xCELLigence. ÐÐ¸Ð·Ð½ÐµÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ ÐºÐ»ÐµÑÐ¾Ðº, Ð¿Ð¾Ð´Ð²ÐµÑÐ¶ÐµÐ½Ð½ÑÑ Ð¼ÐµÑÐ°Ð½Ð¸ÑÐµÑÐºÐ¾Ð¼Ñ Ð¿Ð¾Ð²ÑÐµÐ¶Ð´ÐµÐ½Ð¸Ñ, Ð¾ÑÐµÐ½Ð¸Ð²Ð°Ð»Ð¸ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÑÐµÐ·Ð°Ð·ÑÑÐ¸Ð½-ÑÐµÑÑÐ°. Ð­ÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð³ÐµÐ½Ð¾Ð², Ð±ÐµÐ»ÐºÐ¾Ð²ÑÐµ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´Ð½ÑÐµ ÐºÐ¾ÑÐ¾ÑÑÑ ÑÑÐ°ÑÑÐ²ÑÑÑ Ð² Ð·Ð°Ð¶Ð¸Ð²Ð»ÐµÐ½Ð¸Ð¸ ÑÐ°Ð½Ñ: HAS1, HAS2, ELN Ð¾ÑÐµÐ½Ð¸Ð²Ð°Ð»Ð¸ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ ÐÐ¢-ÐÐ¦Ð . ÐÐ° Ð¾ÑÐ½Ð¾Ð²Ðµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð² ÑÐ°Ð±Ð¾ÑÑ, Ð¼Ð¾Ð¶Ð½Ð¾ Ð·Ð°ÐºÐ»ÑÑÐ¸ÑÑ, ÑÑÐ¾ Ð Ð­Ð ÑÐ¾Ð¼Ð°ÑÐ°, Ð¾Ð³ÑÑÑÐ°, ÑÐ»Ð¾ÑÐµÐ»Ð»Ñ Ð¸ ÑÐ»Ð°Ð¼Ð¸Ð´Ð¾Ð¼Ð¾Ð½Ð°Ð´Ñ Ð½Ðµ Ð¾ÐºÐ°Ð·ÑÐ²Ð°Ð»Ð¸ ÑÐ¸ÑÐ¾ÑÐ¾ÐºÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¸ Ð¾Ð½ÐºÐ¾Ð³ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ, ÑÐ²ÐµÐ»Ð¸ÑÐ¸Ð²Ð°Ð»Ð¸ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ ÑÐ°ÑÐ³ÐµÑÐ½ÑÑ Ð³ÐµÐ½Ð¾Ð² Ð² ÐºÐ»ÐµÑÐºÐ°Ñ ÐºÐ¾Ð¶Ð¸ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð¿Ð¾ÑÐ»Ðµ Ð¸Ñ Ð¼ÐµÑÐ°Ð½Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¿Ð¾Ð²ÑÐµÐ¶Ð´ÐµÐ½Ð¸Ñ Ð¸ Ð¿Ð¾Ð²ÑÑÐ°Ð»Ð¸ Ð¸Ñ Ð¶Ð¸Ð·Ð½ÐµÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ. Ð ÑÐ¾Ð²Ð¾ÐºÑÐ¿Ð½Ð¾ÑÑÐ¸, Ð Ð­Ð ÑÐ°ÑÑÐ¼Ð¾ÑÑÐµÐ½Ð½ÑÑ Ð² Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ Ð¿ÑÐ¾Ð´ÑÑÐµÐ½ÑÐ¾Ð² Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»ÑÐ½Ð¾ Ð¼Ð¾Ð³ÑÑ Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÑÑ ÑÐµÑÐ°Ð¿ÐµÐ²ÑÐ¸ÑÐµÑÐºÐ¾Ðµ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ðµ Ð¿ÑÐ¸ Ð·Ð°Ð¶Ð¸Ð²Ð»ÐµÐ½Ð¸Ð¸ ÑÐ°Ð½Ñ.
description: Plant extracellular vesicles are promising therapeutics for treating skin injuries. The aim of this research work was to evaluate the effect of PEVs on the regeneration of human skin cells after their mechanical damage in vitro. PEVs were isolated by sequential differential ultracentrifugation from microalgae, chlamydomonas (Chlamydomonas reinhardtii CC125) and chlorella (Parachlorella kessleri IPPAS c15), as well as from tomato and cucumber. HaCaT (keratinocytes), DF-2 (fibroblasts), and HEK293T (human embryonic kidney cells) cell monolayers were scratched and co-cultured with PEVs for 24 hours. The effect of PEVs on the proliferative activity of intact cells (cytotoxicity) was analyzed using RTCA xCELLigence. Cell viability of mechanically damaged cells was assessed using the resazurin assay. The expression of genes whose protein derivatives are involved in wound healing: HAS1, HAS2, ELN was evaluated by RT-PCR. Based on the results of the work, it can be concluded that tomato, cucumber, chlorella and chlamydomonas PEVs did not have a cytotoxic or oncogenic effect, increased cell viability and the expression levels of target genes in human skin cells after their mechanical damage. In conclusion, PEVs considered in this work can potentially have a therapeutic effect in wound healing.