Additional file 1 of The regional diversity of gut microbiome along the GI tract of male C57BL/6 mice
Creators: name: Enkhchimeg Lkhagva
name: Hea-Jong Chung
name: Hong, Jinny
name: Tang, Wai Hong Wilson
name: Lee, Sang-Il
name: Seong-Tshool Hong
name: Lee, Seungkoo
Publisher: name: figshare
Year: 2021
Subjects: subject: Biochemistry
subject: Space Science
subject: Microbiology
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Physiology
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: FOS: Chemical sciences
subject: Ecology
subject: Cancer
subject: Inorganic Chemistry
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: FOS: Health sciences
subject: Plant Biology

description: Additional file 1: Figure S1. The photo pictures of the whole GI tracts used in this experiment. (1) Stomach, (2) Duodenum, (3) Jejunum, (4) Ileum, (5) Cecum, (6) Colon. Figure S2. Maximum-likelihood phylogenetic tree comprising the taxa in each location of the GI tract of mouse number 1. The rings of the circular dendrogram represent the family level and the corresponding phylum is depicted in the inner layer and brunch node. (a) Feces, (b) Stomach, (c) Duodenum, (d) Jejunum, (e) Ileum, (f) Cecum, (g) Colon. Figure S3. Maximum-likelihood phylogenetic tree comprising the taxa in each location of the GI tract of mouse number 2. The rings of the circular dendrogram represent the family level and the corresponding phylum is depicted in the inner layer and brunch node. (a) Feces, (b) Stomach, (c) Duodenum, (d) Jejunum, (e) Ileum, (f) Cecum, (g) Colon. Figure S4. Maximum-likelihood phylogenetic tree comprising the taxa in each location of the GI tract of mouse number 3. The rings of the circular dendrogram represent the family level and the corresponding phylum is depicted in the inner layer and brunch node. (a) Feces, (b) Stomach, (c) Duodenum, (d) Jejunum, (e) Ileum, (f) Cecum, (g) Colon. Figure S5. Relative abundance of taxonomic groups of microorganisms occupying in the different GI sections and feces in each mouse. (a) Class, (b) Order, (c) Family, and (d) Genus levels. Figure S6. Comparison of microbial diversity at the different locations of the GI tract in the same mouse by β-diversity analysis. A. Non-metric multidimensional scaling (NMDS). B. 3D Principal coordinate analysis (PCoA). C. 2D Principal coordinate analysis (PCoA). The percentage of variation explained by indicated axis. Figure S7. Ordination plots based on the Bray-Curtis distances in the microbial communities of the GI tracts. 2D stress values were 0.03, 0.29, 0.086 and 0.14 for mouse 1, mouse 2, mouse 3 and all mice respectively. A. Redundancy analysis (RDA), B. ta (DCA), C. Multidimensional scaling (MDS) and D. Correspondence analysis (CA). Figure S8. Correlation of all OTU values at genus level. Heatmap of the microbial composition and relative abundance of all samples based on the Bray–Curtis distance matrix calculated from relative OTU abundances at genus level. Figure S9. Relative abundance of taxonomic groups of microorganisms occupying in the GI tract and feces in each mouse. (a) Phylum, (b) Class, (c) Order, (d) Genus levels.