Supplemental Table 1. Means (± SE) across the experiment for each of the behavioral indices examined.;Supplemental Table 2. Summary of statistical models comparing males with RNAi-hits to all Control males.;Supplemental Table 3. Summary of statistical models comparing all males injected with RNAi (including those unconfirmed as hits) to Control males.;Supplementary Table 4. Summary of model competition for the analyses comparing males with RNAi-hit and Control males.
Creators: name: Lambert, Connor T.
name: Lichter, James B.
name: Perry, Adam N.
name: Castillo, Samuel A.
name: Keane, Brian
name: Cushing, Bruce S.
name: Solomon, Nancy G.
Publisher: name: The Royal Society
Year: 2021
Subjects: subject: Neuroscience
subject: Ecology
subject: FOS: Biological sciences
subject: 60801 Animal Behaviour

description: Supplemental Table 1. Means (± SE) across the experiment for each of the behavioral indices examined. For nest-checking, periods were weeks 6-8, 9-11, and 12-14 and correspond to nest-trapping schedules; grid-trapping data similarly corresponded to grid-trapping schedules and consisted of weeks 8, 11, and 14-15. Radio-tracking periods were weeks 3-6, 7-10, and 11-14. Genetic parentage indices were examined across the entire study rather than for distinct periods. RNAi includes only voles in which ERα knockdown in the MeA was verified, while RNAi (All) includes all voles injected with RNAi, including those in which ERα could not be verified.;Supplemental Table 2. Summary of statistical models comparing males with RNAi-hits to all Control males. The intercept (RNAi male mean), effect (difference of Control from RNAi males or else the effect of survival), standard error of the effect (SE), and N are all from mixed effects models where male treatment is included as a fixed effect. The χ2, df, and p are from likelihood ratio tests comparing the model with male treatment to a null model that does not include treatment. All models were mixed-effects models that included individual vole ID as a random effect, and period was included as a random effect in the models for Residency, Social Monogamy, Max Overlap (grid), Proportion Overlapped (grid). See Table 1 (main text) for details on each index. Note that the analysis for Genetic Monogamy in the text was a Fisher's Exact test; the model presented here is an additional mixed-effects model that still found the same result. *Indicates logistic regression models; the presented intercepts and effects have been transformed to odds ratios.;Supplemental Table 3. Summary of statistical models comparing all males injected with RNAi (including those unconfirmed as hits) to Control males. The intercept (all RNAi male mean), effect (difference of Control from RNAi males or else the effect of survival), standard error of the effect (SE), and N are all from mixed effects models where male treatment is included as a fixed effect. The χ2, df, and p are from likelihood ratio tests comparing the model with male treatment to a null model that does not include treatment. All models were mixed-effects models that included individual vole ID as a random effect, and period was included as a random effect in the models for Residency, Social Monogamy, and Proportion Overlapped (grid). Period was included as a fixed effect interaction with treatment for Max Overlap (grid), as during the last period control males had larger overlaps than all RNAi males. See Table 1 (main text) for details on each index. *Indicates logistic regression models; the presented intercepts and effects have been transformed to odds ratios.;Supplementary Table 4. Summary of model competition for the analyses comparing males with RNAi-hit and Control males. Fixed and random effects (indicated in parentheses), degrees of freedom (df), and AIC values listed for each model. The first model listed under each analysis includes male treatment and the second model is the null model that was compared to the treatment model for each analysis (Table 2). The full model is listed third, followed by the model with treatment and period as a fixed effect and the model with the interaction of treatment and period. For each index, the model with treatment is bolded if it significantly differs from the null model based on a likelihood ratio test; otherwise, the null model is bolded.