title: ÐÐ½Ð°Ð»Ð¸Ð· Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹ Ð² Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð°ÐºÑÐ¸Ð½Ð¾Ð²Ð¾Ð³Ð¾ ÑÐ¸ÑÐ¾ÑÐºÐµÐ»ÐµÑÐ° Ð² Ð¿ÑÐ¾ÑÐµÑÑÐµ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ Ð² ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð»Ð¸Ð½Ð¸ÑÑ Fet-MSC Ð¸ ADH-MSC
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Analysis of changes in the organization of actin cytoskeleton in the process of replicative senescence of FetMSC and ADH-MSC cell lines
Creators: name: ÐÐ¾Ð½ÑÐ°ÑÐ¾Ð²Ð°, ÐÐ°ÑÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: ÐÐ¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð°Ñ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ñ
ÐÐ»ÐµÑÐºÐ° (Ð±Ð¸Ð¾Ð».)
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ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ðµ ÑÑÐ°ÑÐµÐ½Ð¸Ðµ
ÐºÐ»ÐµÑÐ¾ÑÐ½Ð°Ñ Ð¿Ð¾Ð´Ð²Ð¸Ð¶Ð½Ð¾ÑÑÑ
LCFD

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹ Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð°ÐºÑÐ¸Ð½Ð¾Ð²Ð¾Ð³Ð¾ ÑÐ¸ÑÐ¾ÑÐºÐµÐ»ÐµÑÐ° Ð² Ð¿ÑÐ¾ÑÐµÑÑÐµ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ Ð»Ð¸Ð½Ð¸Ð¹ Ð¼ÐµÐ·ÐµÐ½ÑÐ¸Ð¼Ð½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº FetMSC Ð¸ ADH-MSC. ÐÐ»Ñ Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ ÑÐ°Ð·Ð½ÑÑ Ð°ÑÐ¿ÐµÐºÑÐ¾Ð² ÑÑÐ¾Ð³Ð¾ ÑÐ²Ð»ÐµÐ½Ð¸Ñ Ð² ÑÐ°Ð±Ð¾ÑÐµ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð¼ÐµÑÐ¾Ð´Ñ: ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÐºÐ»ÐµÑÐ¾Ðº, ÐºÐ¾Ð½ÑÐ¾ÐºÐ°Ð»ÑÐ½Ð°Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ, Ð¸Ð¼Ð¼ÑÐ½Ð¾ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ðµ Ð¾ÐºÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ðµ, Ð°Ð½Ð°Ð»Ð¸Ð· ÑÑÑÑÐºÑÑÑ Ð°ÐºÑÐ¸Ð½Ð¾Ð²Ð¾Ð³Ð¾ ÑÐ¸ÑÐ¾ÑÐºÐµÐ»ÐµÑÐ° Ð¸ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ Ð¿Ð¾Ð´Ð²Ð¸Ð¶Ð½Ð¾ÑÑÐ¸. ÐÑÐ¸Ð¶Ð¸Ð·Ð½ÐµÐ½Ð½ÑÐµ Ð½Ð°Ð±Ð»ÑÐ´ÐµÐ½Ð¸Ñ Ð·Ð° Ð¿Ð¾Ð´Ð²Ð¸Ð¶Ð½Ð¾ÑÑÑÑ ÐºÐ»ÐµÑÐ¾Ðº, ÑÐ°ÑÐ¿Ð»Ð°ÑÑÐ°Ð½Ð½ÑÑ Ð½Ð° Ð¿Ð»Ð°ÑÑÐ¸ÐºÐµ, Ð² ÑÑÑÐµÑÑÐ¾Ð²ÑÑ ÑÑÐ»Ð¾Ð²Ð¸ÑÑ (Ð² 1%-Ð½Ð¾Ð¹ ÑÑÐ²Ð¾ÑÐ¾ÑÐºÐµ) Ð² ÑÐµÑÐµÐ½Ð¸Ðµ 24 Ñ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð»Ð¸, ÑÑÐ¾ Ð² Ð¿ÑÐ¾ÑÐµÑÑÐµ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ ÑÑÐµÐ´Ð½ÑÑ ÑÐºÐ¾ÑÐ¾ÑÑÑ ÐºÐ»ÐµÑÐ¾Ðº FetMSC ÑÐ½Ð°ÑÐ°Ð»Ð° ÑÐ¸Ð»ÑÐ½Ð¾ ÑÐ²ÐµÐ»Ð¸ÑÐ¸Ð²Ð°ÐµÑÑÑ Ñ 9-Ð³Ð¾ Ð¿Ð¾ 15-Ð¹ Ð¿Ð°ÑÑÐ°Ð¶, Ð·Ð°ÑÐµÐ¼ Ð·Ð½Ð°ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾ Ð¿Ð°Ð´Ð°ÐµÑ Ðº 22-Ð¼Ñ Ð¿Ð°ÑÑÐ°Ð¶Ñ. ÐÐ±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¾, ÑÑÐ¾ Ð¸Ð½ÐºÑÐ±Ð°ÑÐ¸Ñ ÐºÐ»ÐµÑÐ¾Ðº FetMSC Ð½Ð° 22-Ð¼ Ð¿Ð°ÑÑÐ°Ð¶Ðµ Ð² ÑÐµÑÐµÐ½Ð¸Ðµ Ð¾Ð´Ð½Ð¾Ð³Ð¾ ÑÐ°ÑÐ° Ñ ÑÐµÐ»ÐµÐºÑÐ¸Ð²Ð½ÑÐ¼ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾ÑÐ¾Ð¼ ROCK1 (Y-27632) Ð¿ÑÐ¸Ð²Ð¾Ð´Ð¸Ñ Ðº ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ñ ÑÑÐµÐ´Ð½ÐµÐ¹ ÑÐºÐ¾ÑÐ¾ÑÑÐ¸ ÐºÐ»ÐµÑÐ¾Ðº Ð¸ ÑÐ¼ÐµÐ½ÑÑÐµÐ½Ð¸Ñ ÐºÐ¾ÑÑÑÐ¸ÑÐ¸ÐµÐ½ÑÐ° Ð»Ð¾ÐºÐ°Ð»ÑÐ½Ð¾Ð¹ ÑÐ²ÑÐ·Ð½Ð¾Ð¹ ÑÑÐ°ÐºÑÐ°Ð»ÑÐ½Ð¾Ð¹ ÑÐ°Ð·Ð¼ÐµÑÐ½Ð¾ÑÑÐ¸ (LCFD) Ð°ÐºÑÐ¸Ð½Ð¾Ð²Ð¾Ð³Ð¾ ÑÐ¸ÑÐ¾ÑÐºÐµÐ»ÐµÑÐ° ÐºÐ»ÐµÑÐ¾Ðº. ÐÐµÑÐ¾Ð´Ð¾Ð¼ Ð¸Ð¼Ð¼ÑÐ½Ð¾ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð½Ð°Ð¼Ð¸ Ð±ÑÐ»Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð° Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½Ð°Ñ Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ñ Ð±ÐµÐ»ÐºÐ° RhoA Ð½Ð° ÑÐ°Ð·Ð½ÑÑ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð»Ð¸Ð½Ð¸ÑÑ ÐÐ¡Ð. ÐÐ° ÐºÐ»ÐµÑÐºÐ°Ñ Ð»Ð¸Ð½Ð¸Ð¸ FetMSC ÐºÐ¾Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ñ RhoA Ñ ÑÐ´ÑÐ¾Ð¼ Ð¸Ð¼ÐµÐµÑ ÑÐµÐ½Ð´ÐµÐ½ÑÐ¸Ñ ÑÐ¼ÐµÐ½ÑÑÐ°ÑÑÑÑ Ð¿ÑÐ¸ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ Ñ 7-Ð³Ð¾ Ð¿Ð¾ 14-Ð¹ Ð¿Ð°ÑÑÐ°Ð¶. ÐÐ° ÐºÐ»ÐµÑÐºÐ°Ñ Ð»Ð¸Ð½Ð¸Ð¸ ADH-MSC, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð¾Ñ Ð±Ð¾Ð»ÑÐ½Ð¾Ð³Ð¾ Ð²Ð·ÑÐ¾ÑÐ»Ð¾Ð³Ð¾ Ð´Ð¾Ð½Ð¾ÑÐ°, ÐºÐ¾Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ñ RhoA Ñ ÑÐ´ÑÐ¾Ð¼ Ð¸Ð¼ÐµÐµÑ ÑÐµÐ½Ð´ÐµÐ½ÑÐ¸Ñ ÑÐ²ÐµÐ»Ð¸ÑÐ¸Ð²Ð°ÑÑÑÑ Ð¿ÑÐ¸ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ Ñ 8-Ð³Ð¾ Ð¿Ð¾ 17-Ð¹ Ð¿Ð°ÑÑÐ°Ð¶. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ Ð½Ð°Ð¼Ð¸ Ð±ÑÐ»Ð¾ Ð²ÑÑÑÐ½ÐµÐ½Ð¾, ÑÑÐ¾ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ðµ ÑÑÐ°ÑÐµÐ½Ð¸Ðµ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ Ð»Ð¸Ð½Ð¸Ð¸ FetMSC ÑÐ¾Ð¿ÑÐ¾Ð²Ð¾Ð¶Ð´Ð°ÐµÑÑÑ Ð·Ð½Ð°ÑÐ¸ÑÐµÐ»ÑÐ½ÑÐ¼Ð¸ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸ÑÐ¼Ð¸ Ð² ÑÐ°ÑÐ°ÐºÑÐµÑÐµ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ Ð¿Ð¾Ð´Ð²Ð¸Ð¶Ð½Ð¾ÑÑÐ¸; Ð½Ð° Ð¿Ð¾Ð·Ð´Ð½Ð¸Ñ ÑÑÐ°Ð´Ð¸ÑÑ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ ÐºÐ»ÐµÑÐºÐ¸ FetMSC ÑÑÐ°Ð½Ð¾Ð²ÑÑÑÑ Ð±Ð¾Ð»ÐµÐµ ÑÑÐ²ÑÑÐ²Ð¸ÑÐµÐ»ÑÐ½ÑÐ¼Ð¸ Ðº Ð¼Ð¾Ð´ÑÐ»ÑÑÐ¾ÑÐ°Ð¼ Ð°ÐºÑÐ¸Ð½Ð¾Ð²Ð¾Ð³Ð¾ ÑÐ¸ÑÐ¾ÑÐºÐµÐ»ÐµÑÐ°; Ð±ÐµÐ»Ð¾Ðº RhoA Ð¼Ð¾Ð¶ÐµÑ Ð¸Ð¼ÐµÑÑ Ð¾ÑÐ¾Ð±ÑÑ ÑÐ¾Ð»Ñ Ð² Ð¿ÑÐ¾ÑÐµÑÑÐ°Ñ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ Ð¸ Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼Ð¾ ÐµÐ³Ð¾ Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐµÐµ Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ.
description: The given work is devoted to the study of changes in the organization of the actin cytoskeleton in the process of replicative senescence of the mesenchymal stem cell lines FetMSC and ADH-MSC. To study various aspects of this phenomenon, the following methods were used in the work: cell cultivation, confocal microscopy, immunofluorescence staining, analysis of the structures of the actin cytoskeleton and cell motility. Lifetime observations of the motility of cells spread out on plastic under stress conditions (at 1% serum) for 24 h showed that during replicative senescence, the average movement velocity of FetMSC cells first strongly increases from the 9th to the 15th passage, then drops significantly by the 22nd passage. It was found that 1 hour incubation of FetMSC cells at passage 22 with a selective inhibitor of ROCK1 (Y-27632) leads to a decrease in the average cell velocity and a decrease in the coefficient of the local connected fractal dimension (LCFD) of the actin cytoskeleton. We investigated the intracellular localization of the RhoA protein on different MSC cell lines by immunofluorescence analysis. In FetMSC cells, colocalization of RhoA with the nucleus tends to decrease during cultivation from 7th to 14th passage. In ADH-MSC cells obtained from a diseased adult donor, colocalization of RhoA with the nucleus tends to increase during cultivation from 8th to 17th passage. As a result of this work, we found out that the replicative senescence of the FetMSC cell line is accompanied by significant alterations in the pattern of cell motility; at the later stages of replicative senescence, FetMSC cells become more sensitive to actin cytoskeleton modulators; the RhoA protein may have a special role in the processes of replicative aging and its further study is needed.