cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
title: uniDINO: Assay-independent feature extraction for fluorescence microscopy images
Creators: name: Morelli, Flavio M.
name: Kim, Vladislav
name: Hecker, Franziska
name: Geibel, Sven
name: Marín Zapata, Paula A.
Publisher: name: Freie Universität Berlin
Year: 2025
Subjects: subject: High-content imaging
subject: Fluorescence microscopy
subject: Morphological profiling
subject: Self-supervised learning
subject: Representation learning
subject: Deep learning
subject: Computer vision
classificationCode: 004
subject: Datenverarbeitung; Informatik

description: High-content imaging (HCI) enables the characterization of cellular states through the extraction of quantitative features from fluorescence microscopy images. Despite the widespread availability of HCI data, the development of generalizable feature extraction models remains challenging due to the heterogeneity of microscopy images, as experiments often differ in channel count, cell type, and assay conditions. To address these challenges, we introduce uniDINO, a generalist feature extraction model capable of handling images with an arbitrary number of channels. We train uniDINO on a dataset of over 900,000 single-channel images from diverse experimental contexts and concatenate single-channel features to generate embeddings for multi-channel images. Our extensive validation across varied datasets demonstrates that uniDINO outperforms traditional computer vision methods and transfer learning from natural images, while also providing interpretability through channel attribution. uniDINO offers an out-of-the-box, computationally efficient solution for feature extraction in fluorescence microscopy, with the potential to significantly accelerate the analysis of HCI datasets.
РАЗРАБОТКА ПРОГНОЗА РАЗВИТИЯ ТУРИСТИЧЕСКОЙ ОТРАСЛИ ВЬЕТНАМА НА 2021–2025 ГОДЫ
Creators: name: Хуссамов Раил Римович
Publisher: name: Петербургский экономический журнал
Year: 2020
Subjects: международный туризм
развитие туристической отрасли
экономический рост
прогноз
доходы
рекреация

description: Статья посвящена разработке прогноза развития туристической отрасли Социалистической Республики Вьетнам. Использован комплексный анализ к исследованию современных тенденций развития туристической отрасли Вьетнама. Проведен анализ посещения Вьетнама иностранными туристами в страновом разрезе. Приведены экспертные оценки международных организаций относительно роста ВВП Вьетнама. Представлены результаты корреляционного и регрессионного анализа, показатели развития туристической отрасли и ВВП Вьетнама. Особое внимание уделено автором прогнозированию ВВП Вьетнама на период 2021–2025 гг.
ЛИКВИДНОСТЬ И ПЛАТЕЖЕСПОСОБНОСТЬ СЕЛЬСКОХОЗЯЙСТВЕННОЙ ОРГАНИЗАЦИИ: СУЩНОСТЬ И МЕТОДЫ АНАЛИЗА
Creators: name: Золотарев Алексей Анатольевич
name: Бадмаева Дина Гомбоевна
Publisher: name: Петербургский экономический журнал
Year: 2020
Subjects: ликвидность
платежеспособность
коэффициенты ликвидности
денежные потоки

description: Статья посвящена исследованию экономической сущности категорий «ликвидность» и «платежеспособность» организации, выявлению принципиальных отличий и взаимосвязи данных характеристик, систематизации подходов и аналитических показателей для анализа ликвидности и платежеспособности сельскохозяйственной организации. В статье уточняются определения «ликвидность» и «платежеспособность», а также предлагается схема взаимосвязи этих двух понятий. Предлагается новый вариант классификации оборотных активов сельскохозяйственной организации, а также авторская трактовка методов расчета коэффициентов ликвидности сельскохозяйственной организации. На основе исследования фактических значений коэффициентов ликвидности четырнадцати сельскохозяйственных организаций АПК Ленинградской области авторами предложены границы нормативных значений коэффициентов ликвидности с указанием класса коэффициентов. Авторами предложена система коэффициентов для проведения коэффициентного анализа на основе денежных потоков.
Mapping SDGs Technical Supplement: Version 1.0
Creators: name: Houtman, Lily
name: Roth, Robert E.
Publisher: name: Zenodo
Year: 2021

description: These tutorials pair with the open-source book Mapping for a Sustainable World. The book teaches principles of cartography through the United Nations’ 17 Sustainable Development Goals. Mapping for a Sustainable World was developed as a collaboration between the United Nations and the International Cartographic Association with the University of Wisconsin Cartography Lab supporting creation of the book layout and all maps and graphics through National Science Foundation CAREER Grant #1555267. All tutorials demonstrate map design using the open access mapping software QGIS. Thus, the objective of these tutorials is to enhance the cartographic design thinking described in Mapping for a Sustainable World with technical map software training, putting concepts into practice. The tutorials were developed by Lily Houtman and Robert E. Roth of the University Wisconsin Cartography Lab under NSF #1555267 and are freely available for personal or classroom/workshop use following a CC BY-NC license.
Supply chain optimization of forest-based biomass for gasification considering uncertainties
Creators: name: Ahmadvand, Sahar
Publisher: name: University of British Columbia
Year: 2023

description: Producing energy from forest-based biomass could aid the transitions in energy and forest sectors to replace fossil fuels; reduce emissions and wastes; and diversify product portfolios and revenue streams. Optimizing the economic and environmental performance of biomass supply chains can help realize these benefits. Besides optimizing the supply chain, investigating other factors, e.g., government incentives and carbon and energy pricing, which contribute to success or failure of forest-based biomass projects, is beneficial. The supply chain of forest-based biomass includes different activities that are cost- and emission-intensive and involve uncertainties. Unlike the majority of studies on forest-based biomass supply chain planning that focused on long-term and deterministic optimization, this dissertation aims to optimize the supply chain of syngas production at tactical level considering uncertainties. Thus, a multi-period bi-objective robust optimization model is developed and applied to the case of a British Columbia Kraft pulp mill that would produce syngas to fuel their lime kiln. The objectives are to minimize costs and emissions while optimizing the monthly biomass procurement, storage, and preprocessing decisions. Robust optimization with an adjustable budget of uncertainty is used to model the uncertainties in biomass supply and cost. Robust Pareto-optimal solutions are obtained that demonstrate a trade-off between objectives. The cost and emissions of robust solutions are on average 68% and 41% higher than those of the deterministic solutions. However, unlike the robust solution, the deterministic solution becomes infeasible in 98% of simulated future scenarios. Besides logistics costs, other factors (e.g., capital investment, government funding, fuel prices, and carbon tax) determine the financial viability of biomass projects. A previous techno-economic feasibility study for the same case revealed that despite a short payback period and positive net present value, the syngas price and carbon tax would result in negative annual cashflows halfway through project’s lifespan. Thus, the pulp mill would be reluctant to invest in this project despite all its potential, especially emission savings. To improve the financial attractiveness of this project for the pulp mill, an optimization model is developed that shows an optimal syngas price of 27 $/GJ, increasing by 0.49¢ annually, would make all cashflows positive.
The story of fitting in : cultural fit and subjective well-being
Creators: name: Bahng, Jungwun Miranda
Publisher: name: University of British Columbia
Year: 2023

description: The present study explored the relationship between cultural fit and subjective well-being. Using data from wave 7 of the World Values Survey, which sampled from 94,278 residents in 64 nations, we examined the extent to which cultural fit, or the fit between an individual’s cultural values and their country’s averaged cultural values, predicts their composite well-being using multilevel modelling. Results showed a significant effect of individual cultural fit predicting composite well-being within countries. Moderation analyses were conducted using national-level tightness, individualism, relational mobility, and cultural heterogeneity. The national-level tightness of a country was the only moderator that consistently exhibited a positive effect on the relationship between cultural fit and subjective well-being, highlighting the importance of considering cultural context when investigating cultural fit and well-being. Exploratory analyses were also conducted. We conclude with a discussion of the relevance and limitations of our findings.
title: Molecular markers for Psocoptera species identification
Creators: name: Ceruti, F. C.
name: Lazzari, S. M. N.
Publisher: name: Julius Kühn-Institut
Year: 2010
Subjects: subject: AFLP
subject: Liposcelididae
subject: psocids
subject: stored-product pests

description: Julius-Kühn-Archiv; 425; 90
Map #117, Site 5MT10711, Pit Room 110, Nonstructure 120, and Arbitrary Unit 105, Stratigraphic Profile
Creators: name: DeVos, Jim
Year: 2018
Subjects: subject: Southwestern Colorado
valueUri: https://core.tdar.org/browse/geographic-keyword/2810/southwestern-colorado
subject: Southwestern US
valueUri: https://core.tdar.org/browse/geographic-keyword/84604/southwestern-us
subject: Basketmaker III
valueUri: https://core.tdar.org/browse/temporal-keyword/78/basketmaker-iii
subject: 5MT10711
valueUri: https://core.tdar.org/browse/site-name/118032/5mt10711
subject: Ridgeline Site
valueUri: https://core.tdar.org/browse/site-name/118031/ridgeline-site

description: Site 5MT10711, Pit Room 110, Nonstructure 120, and Arbitrary Unit 105, Stratigraphic Profile
СОВЕРШЕНСТВОВАНИЕ ДЕНЕЖНО-КРЕДИТНОЙ ПОЛИТИКИ В УСЛОВИЯХ НИЗКИХ ПРОЦЕНТНЫХ СТАВОК
Creators: name: Земцова Надежда Владиславна
name: Леонов Михаил Витальевич
Publisher: name: Петербургский экономический журнал
Year: 2020
Subjects: Центральный банк
денежно-кредитная политика
низкая процентная ставка
количественное смягчение
национальная цифровая валюта
кривая доходности
общественные издержки

description: Авторами раскрыты основные стратегии денежно-кредитной политики в условиях низких процентных ставок. В статье систематизированы специальные инструменты денежно-кредитной политики, такие как операции количественного смягчения и установления границ процентных ставок. Описаны возможности повышения эффективности денежно-кредитной политики низких процентных ставок при введении в обращение национальной цифровой валюты. Обосновано, что при условии введения в обращение национальной цифровой валюты могут быть минимизированы общественные издержки. В результате сделан вывод о том, что возрастание роли Центрального банка в перераспределении финансовых ресурсов в экономике позволит повысить эффективность трансмиссии денежнокредитной политики.
Map #463, Site 5MT10647, Midden 502, Arbitrary Unit 501, and Arbitrary Unit 503
Creators: name: DeVos, Jim
Year: 2018
Subjects: subject: Southwestern Colorado
valueUri: https://core.tdar.org/browse/geographic-keyword/2810/southwestern-colorado
subject: Southwestern US
valueUri: https://core.tdar.org/browse/geographic-keyword/84604/southwestern-us
subject: Basketmaker III
valueUri: https://core.tdar.org/browse/temporal-keyword/78/basketmaker-iii
subject: Pueblo I
valueUri: https://core.tdar.org/browse/temporal-keyword/54/pueblo-i
subject: Pueblo II
valueUri: https://core.tdar.org/browse/temporal-keyword/55/pueblo-ii
subject: 5MT10647
valueUri: https://core.tdar.org/browse/site-name/118013/5mt10647
subject: Dillard Site
valueUri: https://core.tdar.org/browse/site-name/118012/dillard-site

description: Site 5MT10647, Midden 502, Arbitrary Unit 501, and Arbitrary Unit 503
title: Tricorynus rudepunctatus (Pic) (Coleoptera: Anobiidae): diagnosis and damage
Creators: name: Ceruti, F. C.
name: Lazzari, S. M. N.
name: Neto, G. H. R.
Publisher: name: Julius Kühn-Institut
Year: 2010
Subjects: subject: Anobiids
subject: insect identification
subject: morphological characters
subject: urban pest

description: Julius-Kühn-Archiv; 425; 91-96
МЕХАНИЗМ УПРАВЛЕНИЯ ВАЛЮТНЫМИ РИСКАМИ ЭКОНОМИЧЕСКОЙ СИСТЕМЫ
Creators: name: Комаров Сергей Михайлович
Publisher: name: Петербургский экономический журнал
Year: 2020
Subjects: инфраструктура управления валютным риском
процесс управления валютным риском
экономическая система на основе международного аутсорсинга
участник экономической системы

description: В статье рассмотрено понятие «международный аутсорсинг» и исследованы особенности управления валютным риском в организациях. В работе выполняется анализ принципов и определений, изложенных в нормативных актах, научных работах по финансовому риску, международному аутсорсингу. Автор рассматривает организацию как экономическую систему, а также особенности управления валютными рисками на основе комплексного подхода. Процесс управления валютным риском представлен как механизм, учитывающий комплексное взаимодействие всех элементов инфраструктуры, процесса управления риском. Также автор предлагает использовать поэтапную оценку валютного риска в процессе управления им в организации и разделять риск по его актуальности в зависимости от ситуации. Работа имеет методическую ценность для организаций, включенных в экономическую систему, осуществляющих деятельность на основе международного аутсорсинга и участвующих в международном разделении труда вне зависимости от механизма формирования экономической системы.
Map #14, Site 5MT10718, Pithouse 107, Surface 1
Creators: name: DeVos, Jim
Year: 2018
Subjects: subject: Southwestern Colorado
valueUri: https://core.tdar.org/browse/geographic-keyword/2810/southwestern-colorado
subject: Southwestern US
valueUri: https://core.tdar.org/browse/geographic-keyword/84604/southwestern-us
subject: Pueblo I
valueUri: https://core.tdar.org/browse/temporal-keyword/54/pueblo-i
subject: 5TM10718
valueUri: https://core.tdar.org/browse/site-name/118028/5tm10718
subject: 5MT10718
valueUri: https://core.tdar.org/browse/site-name/118027/5mt10718

description: Site 5MT10718, Pithouse 107, Surface 1
title: Early Hakka recordings in the Berlin Sound Archive: speaker history and corpus contents
Creators: name: Klöter, Henning
name: Bartsch, Leif Jannik
Publisher: name: Freie Universität Berlin
Year: 2025
Subjects: subject: Chinese Hakka
subject: Berlin Sound Archive
subject: voice recording
subject: Basel Mission
subject: missionary and colonial linguistics
subject: native speaker
classificationCode: 410
subject: Linguistik

description: The Sound Archive (Lautarchiv) of the Humboldt-Universität zu Berlin preserves some 30 min of audio recordings documenting the historical usage of the Chinese Hakka dialect. Produced in 1926, these recordings are among the earliest extant audio witnesses to a Chinese language or dialect. This article serves the double purpose of situating the recordings within their historical context of production and offering a preliminary assessment of their linguistic content. The historical contextualisation centres on the personal history of the speaker whose voice was captured in the recordings. Whilst the native speakers collaborating with linguists tended to be ignored in this period, it is possible in this case to identify the speaker by name, and to reconstruct his life story in some detail by utilising archival records of the Basel Mission. The recordings encompass phonetics, morpho-syntax, conversations and basic narratives, thereby reflecting a didactic approach that aligns with the Basel Mission’s dedication to language instruction and documentation.
Additional file 2 of Effects of a behavioral intervention on physical activity, diet, and health-related quality of life in pregnant women with elevated weight: results of the HIPP randomized controlled trial
Creators: name: Wilcox, Sara
name: Liu, Jihong
name: Turner-McGrievy, Gabrielle M.
name: Boutté, Alycia K.
name: Wingard, Ellen
Publisher: name: figshare
Year: 2023
Subjects: subject: Medicine
subject: Physiology
subject: FOS: Biological sciences
subject: Biotechnology
subject: Sociology
subject: FOS: Sociology
subject: Science Policy
subject: Mental Health

description: Additional file 2.
title: How teacher agency adapted to child competencies and teacher communion relate to student needs fulfillment and motivation
Creators: name: Koeppen, Karoline
name: Kreutzmann, Madeleine
name: Roswag, Malte
name: Frühauf, Madita
name: Valcárcel Jiménez, María
name: Hannover, Bettina
Publisher: name: Freie Universität Berlin
Year: 2025
Subjects: subject: Teacher-child relationship
subject: Circumplex
subject: Agency
subject: Communion
subject: Child self-determined motivation
classificationCode: 370
subject: Bildung und Erziehung

description: A teacher who behaves warm and affectionate towards a child (strong communion) is more beneficial for the student's motivation than one who expresses feelings of distance or rejection (weak communion). Additionally, adaptive teaching where instructional support, guidance, and supervision are adjusted to the individual child's competencies (adaptive agency) has been proven beneficial for student learning. We modelled teacher behavior in an interpersonal circumplex which allows teacher communion and adaptive agency as well as their effects on the student to be described simultaneously. We investigated the micro-system of the child-teacher dyad and measured child competencies via standardized tests. We predicted that teacher-reported strong communion and adaptive agency fosters child-reported need fulfillment and motivation. Results on 1769 elementary school children and their 77 teachers showed that strong communion and – irrespective of child competencies - weak agency strengthened need fulfillment and motivation. We discuss implications for self-determination theory and adaptive teaching.
title: Optimizing cation–π force fields for molecular dynamics studies of competitive solvation in conjugated organosulfur polymers for lithium–sulfur batteries
Creators: name: Gayen, Diptesh
name: Schütze, Yannik
name: Groh, Sébastien
name: Dzubiella, Joachim
Publisher: name: Freie Universität Berlin
Year: 2025
Subjects: subject: lithium–sulfur batteries
subject: molecular dynamics
subject: conjugated organosulfur polymers
classificationCode: 540
subject: Chemie und zugeordnete Wissenschaften

description: Lithium–sulfur (Li/S) batteries are emerging as a next-generation energy storage technology due to their high theoretical energy density and cost-effectiveness. π-Conjugated organosulfur polymers, such as poly(4-(thiophene-3-yl)benzenethiol) (PTBT), have shown promise in overcoming challenges such as the polysulfide shuttle effect by providing a conductive framework and enabling sulfur copolymerization. In these cathodes, cation–π interactions significantly influence Li+ diffusion and storage properties in π-conjugated cathodes, but classical OPLS-AA force fields fail to capture these effects. This study employs a bottom-up approach based on density functional theory (DFT) to optimize the nonbonded interaction parameters (OPLS-AA/corr.), particularly for the Li+–π interactions with the PTBT polymer. Following prior work, we used an ion-induced dipole potential to model the cation–π interactions. The impact of the solvent on the PTBT monomers was examined by computing the potential of mean force (PMF) between PTBT monomers and Li+ ions in both explicit and implicit solvents using the Boltzmann inversion of probability distributions close to room temperature. In the implicit solvent case, the magnitude of the binding free energy decreased with increasing dielectric constant, as the dominant electrostatics scaled with the dielectric constant. In contrast, in the explicit solvent case, considering the mixtures of organic solvent DME and DOL, the binding free energy shows minimal dependence on solvent composition due to the competing interaction of TBT and Li+ with the solvent molecules. However, increasing salt concentration decreases the binding free energy due to Debye–Hückel screening effects. In general, this work suggests that the optimized parameters can be widely used in the simulation of polymers in electrolytes for the Li/S battery to enhance the representation of cation–π interactions for a fixed charge force field.
ПРОГРАММНОЕ ОБЕСПЕЧЕНИЕ МОДЕЛИРОВАНИЯ АРХИТЕКТУРЫ УПРАВЛЕНИЯ ПРОМЫШЛЕННЫМ ПРЕДПРИЯТИЕМ
Creators: name: Стельмашонок Виталий Леонидович
name: Еникеева Лилия Аубакировна
name: Стельмашонок Елена Викторовна
Publisher: name: Петербургский экономический журнал
Year: 2020
Subjects: система управления
архитектура управления предприятием
моделирование архитектуры
программное обеспечение
архитектура данных
карта доверия
удовлетворенность клиентов

description: Данная статья посвящена анализу программного обеспечения моделирования архитектуры системы управления предприятием. Проведено соответствие существующего программного обеспечения современным задачам моделирования архитектуры системы управления крупными предприятиями. Для анализа соответствия использованы мнения экспертов и карта доверия. Карта доверия позволяет сравнивать программные продукты с позиции выстраивания архитектуры системы управления предприятием на основе аутентифицированных данных конечных пользователей. Современное программное обеспечение моделирования архитектуры управления промышленным предприятием необходимо для поддержки системы управления промышленным предприятием.
ПРИМЕНЕНИЕ КОНЦЕПЦИИ BLOCK CHAIN В ЦЕЛЯХ СОВЕРШЕНСТВОВАНИЯ СИСТЕМЫ УПРАВЛЕНИЯ КАЧЕСТВОМ НА ПРЕДПРИЯТИИ
Creators: name: Лебедева Татьяна Сергеевна
name: Яковлев Андрей Анатольевич
Publisher: name: Петербургский экономический журнал
Year: 2020
Subjects: система менеджмента качества
бережливое производство
криптоэнергия
блокчейн
облачные вычисления
когнитивные технологии
интернет вещей

description: Несмотря на активную пропаганду необходимости акцентированного внимания производителей к запросам потребителя, наличия нормативной базы и системы сертификации качества, регулярного прохождения производителями процедуры подтверждения соответствия качества товаров и услуг требованиям технических регламентов, современный потребительский рынок России предельно засорен большим количеством некачественных товаров и услуг. Все это ставит под сомнение целесообразность использования в России систем качества и требует создания более совершенной системы управления качеством, введения в повседневную практику эффективного механизма, исключающего возможность появления на рынках некачественных товаров и услуг. В работе рассмотрена возможность оптимизации систем качества на основе создания равных информационных условий от производителя до потребителя. В условиях перехода к концепциям цифровой экономики экономическая практика доказывает эффективность применения технологии «Распределенный реестр».
Construction of functionalized nitrogen heterocycles using 2-(diphenylphosphoryl)oxygen-1,3-butadienes in multicomponent [4+2] cycloaddition reactions
Creators: name: He, Qiuyu
Publisher: name: University of British Columbia
Year: 2023

description: This dissertation presents research work on hetero-Diels-Alder reactions using 2-diphenylphosphoryloxy-1,3-butadienes and imines for the construction of six-membered nitrogen heterocycles. Chapter 1 provides an overview of hetero-Diels-Alder reactions that primarily focuses on imines and enol-based dienes. Chapter 2 describes hetero-Diels-Alder reactions between 2-diphenylphosphoryloxy-1,3-butadienes and imines under the promotion of iron(III) chloride. Twelve examples of 2-diphenylphosphoryloxy-1,3-butadienes were synthesized, and eighteen cycloaddition products were constructed in 30–91% isolated yields. Relative configurations of products formed in diastereomeric mixtures were analyzed by 2-dimensional NMR spectroscopy, and the diastereomeric ratios ranged from 2:1 to >20:1. The utility of the enol phosphate functionality was demonstrated in manipulations of the cycloaddition products in transition metal catalyzed cross-coupling reactions. Chapter 3 describes the multicomponent hetero-Diels-Alder reactions using dienes from Chapter 2, amines, and aldehydes through in situ generation of iminium species under the promotion of boron trifluoride diethyl etherate. The reactions were carried in non-distilled dichloromethane without the use of inert atmosphere. Thirty-one examples of cycloaddition products were presented with a diverse scope of aromatic aldehydes, aliphatic aldehydes, N-sulfonyl amines, and N-carbamoyl amines. Chapter 4 describes a sequence of cycloaddition–cross-coupling–oxidation reactions to construct functionalized pyridines. The N-Fmoc substituted cycloaddition products were subjected to Pd(0)-catalyzed Kumada coupling reactions with varied Grignard coupling partners. The Fmoc protecting group in the cross-coupling products was deprotected using piperidine. A subsequent addition of 2,3-dichloro-5,6-dicyano-1,4-benzoquinone oxidized the heterocyclic ring to an aromatic pyridine structure. Twenty examples of disubstituted pyridines and one example of trisubstituted pyridine were constructed in 17–88% yields over two synthetic steps. Twelve examples of fluorescent 4-biphenylpyridines were constructed and could potentially be applied in light-luminating materials. Their wavelengths of maximum emission lied in the range of 349–389 nm.
Map #34, Site 5MT10709, Midden 101, Nonstructure 109, and Arbitrary Unit 107, Stratigraphic Profile
Creators: name: DeVos, Jim
Year: 2018
Subjects: subject: Southwestern Colorado
valueUri: https://core.tdar.org/browse/geographic-keyword/2810/southwestern-colorado
subject: Southwestern US
valueUri: https://core.tdar.org/browse/geographic-keyword/84604/southwestern-us
subject: Basketmaker III
valueUri: https://core.tdar.org/browse/temporal-keyword/78/basketmaker-iii
subject: 5MT10709
valueUri: https://core.tdar.org/browse/site-name/118022/5mt10709
subject: Portulaca Point
valueUri: https://core.tdar.org/browse/site-name/118021/portulaca-point

description: Site 5MT10709, Midden 101, Nonstructure 109, and Arbitrary Unit 107, Stratigraphic Profile
Successful gene editing in a novel mouse model of aniridia towards prevention of blindness
Creators: name: Mirjalili Mohanna, Seyedeh Zeinab
Publisher: name: University of British Columbia
Year: 2023

description: Aniridia is a rare eye disorder with no cure and is caused by pathogenic variants in the paired box 6 (PAX6) gene. Patients have low vision at birth due to retinal foveal hypoplasia, which typically progresses to blindness by early adulthood due to corneal clouding and glaucoma. Since aniridia is caused by PAX6 haploinsufficiency, one potential approach to treating it is targeted CRISPR-based gene editing. Preclinical studies in an appropriate animal model are important first steps towards the translation of the research into a clinical trial. Thus, in Chapter 2, we inserted a 3xFLAG tag on the Pax6 small eye (Sey) allele, creating a novel mouse model for aniridia that is ideal for testing CRISPR strategies. Next, we developed a CRISPR strategy in vitro and subsequently evaluated its efficacy in vivo in the germline of our new mouse. We showed that our CRISPR strategy corrected the Sey variant in the mouse germline, resulting in the full rescue of the eye phenotype. Next, we worked towards developing a somatic therapy, the success of which relies on effective delivery of CRISPR components to therapeutically significant cells. In Chapter 3, we tested a novel lipid nanoparticle-based platform, and successfully edited cultured mouse cortical neurons. We then directly injected LNP-CRISPR into the mouse cornea to evaluate in vivo delivery. We demonstrated wide-spread gene editing of corneal stromal and endothelial cells but not the PAX6-expressing epithelial or limbal stem cells (LSCs). Hence, in Chapter 4, we shifted our focus to recombinant adeno-associated viruses (rAAVs), a more conventional delivery vector for CRISPR. We carried out a series of experiments to test viral capsid, dose, and route of administration. We also investigated if functional LSCs are present in the already cloudy aniridic mouse corneas and whether rAAV can transduce them. We reported unexpected ataxia and lethality in mice after intraocular rAAV-PHP.B injections. We also showed that intrastromal and intravitreal delivery of rAAV9 transduced functional LSCs, as well as all PAX6-expressing retinal cell types in aniridic eye, respectively, with no observed adverse events, making rAAV9 the capsid of choice for the future gene therapy development for aniridia.
title: Fractional Kolmogorov Equations with Singular Paracontrolled Terminal Conditions
Creators: name: Kremp, Helena
name: Perkowski, Nicolas
Publisher: name: Freie Universität Berlin
Year: 2025
Subjects: subject: Kolmogorov equation
subject: Fractional Laplace operator
subject: Paracontrolled distributions
subject: Singular terminal conditions
classificationCode: 510
subject: Mathematik

description: We consider backward fractional Kolmogorov equations with singular Besov drift of low regularity and singular terminal conditions. To treat drifts beyond the so-called Young regime, we assume an enhancement assumption on the drift and consider paracontrolled terminal conditions. Our work generalizes previous results on the equation from Cannizzaro and Chouk (Ann Probab 46:1710–1763, 2018), Kremp and Perkowski (Bernoulli 28:1757–1783, 2022. https://doi.org/10.3150/21-BEJ1394) to the case of singular paracontrolled terminal conditions and simultaneously treats singular and non-singular data in one concise solution theory. We introduce a paracontrolled solution space that implies parabolic time and space regularity on the solution without introducing the so-called modified paraproduct from Gubinelli and Perkowski (Commun Math Phys 349:165–269, 2017). The tools developed in this article apply for general linear PDEs that can be tackled with the paracontrolled ansatz.
title: Necessary and sufficient conditions for distances on the real line
Creators: name: Labora, Daniel Cao
name: Fernández, Francisco J.
name: Tojo, F. Adrián F.
name: Villanueva Mariz, Carlos
Publisher: name: Freie Universität Berlin
Year: 2025
Subjects: subject: distances
subject: real line
subject: integration
classificationCode: 510
subject: Mathematik

description: When dealing with certain mathematical problems, it is sometimes necessary to show that some function induces a metric on a certain space. When this function is not a well renowned example of a distance, one has to develop very particular arguments that appeal to the concrete expression of the function in order to do so. The main purpose of this work is to provide several sufficient results ensuring that a function of two variables induces a distance on the real line, as well as some necessary conditions, together with several examples that show the applicability of these results. In particular, we show how a hypothesis about the sign of the cross partial derivative of the candidate to distance is helpful for deriving such kind of results.
Assessing production of phytolith and phytolith occluded carbon in above-ground biomass of intensively cultivated rice ecosystems in India
Creators: name: Anjum, Mohsina
name: Nagabovanalli, Prakash Basavarajappa
Publisher: name: Taylor & Francis
Year: 2021
Subjects: subject: Physiology
subject: FOS: Biological sciences
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Ecology
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified

description: Carbon (C) occluded in phytoliths is stable and an important source of long-term C storage into the agroecosystems. As a silicon (Si) accumulator crop, rice produces a large amount of phytolith occluded carbon (PhytOC) and which plays a crucial role in the coupled biogeochemical cycle of C and Si. In this study, we sampled above ground biomass from 22 intensively cultivated rice ecosystems to evaluate the rate of C sequestration through phytoliths. Depending on the rice varieties there exist a great variability in PhytOC content (0.12–0.26%) across the rice ecosystems. The collected samples were further classified into three groups: viz., long (>140 days), medium (125–140 days) and short duration (<125 days). The above-ground net primary productivity (ANPP) varied from 5174 to 13,394 kg ha−1 and recorded significantly (p p 2 = 0.45) between ANPP and crop duration. With the increase in varietal duration, phytolith content was non-significant (p > 0.05) whereas, the PhytOC content found to be significant (p 140 days) varieties showed 69.7% and 164.0% higher PhytOC sequestration flux over medium and short-duration varieties, respectively. Our results suggest that the Indian rice ecosystems sequester 0.95 Tg CO2 yr−1 through Phytoliths, which is equivalent to the 15.5% of PhytOC sink in the global rice field. Considering the PhytOC flux of relatively higher PhytOC accumulating long duration varieties, the annual PhytOC sink rate in the Indian rice ecosystems can be increased to ∼4 folds over the current rate. It was revealed that the selection of higher PhytOC accumulating long duration varieties can enhance the phytolith C sequestration.
Carbon pricing and supporting policy tools for deep decarbonization; case of electricity generation of Sri Lanka
Creators: name: Herath, H. M. S. Amanda
name: Jung, Tae Yong
Publisher: name: Taylor & Francis
Year: 2021
Subjects: subject: Medicine
subject: Ecology
subject: FOS: Biological sciences
subject: Developmental Biology
subject: Science Policy
subject: 111714 Mental Health
subject: FOS: Health sciences
subject: Computational Biology

description: This study internalizes carbon pricing and other regulatory policy tools for decarbonizing the electricity generation mix of Sri Lanka. Carbon Pricing is shifted to the supply side as a decision-making tool. We consider $10/tCO2, $20/tCO2, $30/tCO2, $44/tCO2, $130/tCO2, and $297/tCO2 as potential carbon prices. Other regulatory policy tools, such as mandatory renewable energy (RE) development, fuel switching, fossil fuel phasing out, fossil fuel development moratoriums, and mandatory RE utilization, are included as possible supporting tools. Three capacity-development scenarios and three optimization models with three objective functions are designed and implemented in the General Algebraic Modeling System (GAMS). Six policy options are presented for the Sri Lankan context considering cost, emission, and diversification. Results suggest a carbon price that falls between $10/tCO2-$44/tCO2 as suitable for Sri Lanka. The study contributes to the ongoing debate on the effectiveness of carbon pricing by empirically testing the effectiveness of carbon prices in the electricity generation planning of a developing country. The findings support the emerging consensus of adopting other regulatory policy tools along with carbon prices to achieve deep decarbonization. The study also assesses the role LNG could play in a developing country’s electricity generation mix. We recommend transitioning from coal to LNG as an intermediate decarbonization policy option until the RE capacities are developed to make the near-zero emission mix a reality.
Therapeutic potential of bioactive compounds from Punica granatum extracts against aging and complicity of FOXO orthologue DAF-16 in Caenorhabditis elegans
Creators: name: Chaubey, Mukesh G.
name: Chauhan, Anita P.
name: Chokshi, Pooja R.
name: Amin, Rahi S.
name: Patel, Stuti N.
name: Madamwar, Datta
name: Rastogi, Rajesh P.
name: Singh, Niraj Kumar
Publisher: name: IfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmund
Year: 2021
Subjects: Punica granatum
C. elegans
antioxidants
anti-aging
free radicals
oxidative stress

description: Some natural fruits have significant importance in improving health which provides many nutritional supplements essential to maintain proper metabolism with the age. In this study, phytochemical screening of extract (methanolic) of Punica granatum arils, outer and inner peels was confirmed by the respective spot tests. Quantification of phytochemical constituents revealed the plentiful of total phenols in the outer peels in comparison to inner peels and juice whereas total flavonoids and vitamin C are abundant in inner peel and juice, respectively. High-performance liquid chromatography, Gas chromatography along with mass spectrometry and Fourier-transform infrared spectroscopy analysis revealed the presence of compound 9, 17-octadecadienal, (Z) in the outer/inner peels. A compound N-hexadecanoic acid was also observed in the outer peels. Extracts from every section of the fruits were comprehensively evaluated for their antioxidant activity. Contrary to fruit aril juice, the extracts of outer and inner peels exhibited significant and dose-dependent in vitro antioxidant and radical-scavenging potentials. The supplementation of P. granatum extracts (PGEs) significantly enhanced the lifespan of C. elegans. The protective effect of PGEs was also observed against oxidative stress in C. elegans. Additionally, the involvement of FOXO orthologue DAF-16 dependent longevity was obtained with PGEs (outer peel and inner peel) fed TJ356 worms. Overall, the results indicate the vital role of PGEs especially the extracts of outer peels in life-saving mechanisms of C. elegans by virtue of their antioxidant asset and life-prolonging effects via daf-16 dependent Insulin signaling pathway.
description: EXCLI Journal; 20:Doc80; ISSN 1611-2156
title: Hylotrupes bajulus (L.) (Col., Cerambycidae): nutrition and attacked material
Creators: name: Chiappini, E.
name: Molinari, P.
name: Busconi, M.
name: Callegari, M.
name: Fogher, C.
name: Bani, P.
Publisher: name: Julius Kühn-Institut
Year: 2010
Subjects: subject: cellulose
subject: starch
subject: frass
subject: mouth apparatus
subject: mandible

description: Julius-Kühn-Archiv; 425; 97-103
Map #413, Site 5MT10647, Nonstructure 110, Feature 1
Creators: name: DeVos, Jim
Year: 2018
Subjects: subject: Southwestern Colorado
valueUri: https://core.tdar.org/browse/geographic-keyword/2810/southwestern-colorado
subject: Southwestern US
valueUri: https://core.tdar.org/browse/geographic-keyword/84604/southwestern-us
subject: Basketmaker III
valueUri: https://core.tdar.org/browse/temporal-keyword/78/basketmaker-iii
subject: Pueblo I
valueUri: https://core.tdar.org/browse/temporal-keyword/54/pueblo-i
subject: Pueblo II
valueUri: https://core.tdar.org/browse/temporal-keyword/55/pueblo-ii
subject: 5MT10647
valueUri: https://core.tdar.org/browse/site-name/118013/5mt10647
subject: Dillard Site
valueUri: https://core.tdar.org/browse/site-name/118012/dillard-site

description: Site 5MT10647, Nonstructure 110, Feature 1
Treatment of bone infections in children in low-income countries – A practical guideline based on clinical cases
Creators: name: Loro, AntoniO
name: Fulvio, FranceschI
name: Alt, Volker
Publisher: name: Universität Regensburg
Year: 2023
Subjects: subject: Bone, Infection, Pediatric, Low-income, Osteomyelitis, Fracture-related infection,Fri, Sequestrum, External fixation, Debridement, Involucrum, Sepsis
subject: 610 Medizin

description: In low-income countries (LIC), the management of bone infections is a huge challenge. A high number of patients
are in the pediatric age group. Children and adolescents exhibit good bone healing potential offering treatment
options that mainly rely on the biological response of the infected bone. The purpose of this article is to highlight
treatment options for these patients in LIC, which is based on clinical cases that illustrate the principles of the
treatment, focusing on bone reaction and healing potential.
First, identification of emergency cases is of importance. Sepsis of the patient due to bone infections is a lifethreatening
disease that requires immediate surgical attention with abscess incision. It should be tailored to the
surgeon’s experience and local conditions to avoid unwanted complications, such as excessive bleeding, fracture
or bone loss.
In non-septic patients, uncomplicated cases should be distinguished from complicated cases as the first might
often require only abscess incision, particularly in small children, without any other major surgical intervention.
Biomechanical stability and bone formation capacity, soft tissue conditions and joint involvement are decisive
factors differentiating uncomplicated from complicated cases. Central treatment column is the immobilization of
the infected bone with simple methods, such as plaster of Paris, braces or external fixation. This is intended to
provide sufficient stability to allow for new bone formation that subsequently downsizes the infection site and
that can bridge previously infected non-union sites or bone defects. In most cases, antibiotic treatment is not
performed as antibiotics are not available or affordable.
Severe soft tissue defects remain a major challenge as microvascular surgical experience is often required for
reliable coverage, for which referral to one of the very few specialized centers is recommended. Major bone
defects should also be treated in centers with sufficient expertise for bone reconstruction procedures. Regular
follow-ups are important to ensure healing and to avoid aggravation of the disease.
Encouraging success rates can be achieved by these treatment principles. However, it should not be forgotten
that poverty in these countries, including limited access to health care, remains one of the world’s most
important problems.
Effect of calorie restriction on motivation to exercise and the steroid profile of female rats
Creators: name: Liu, Megan
Publisher: name: University of British Columbia
Year: 2023

description: Exercise is beneficial for the body, including the brain. Despite extensive research on the benefits of exercise, the neural and endocrine mechanisms controlling the motivation to exercise remain largely unknown. Wheel running is inherently rewarding for rodents and can be used to model voluntary exercise. Steroid hormones such as corticosterone, testosterone, and 17β-estradiol influence wheel running. Previous rodent studies have assessed wheel running performance, but this measure fails to distinguish between physical ability and motivation. The primary objective of this study was to validate a novel operant task for specifically measuring the motivation to run in rats. Females run more than males, and calorie restriction increases wheel running in rats. Here, adult female rats were calorie restricted (CR) or fed ad libitum (AL), and their motivation to exercise was assessed using a novel progressive ratio task in which lever pressing was paired with the opportunity to wheel run for 1 min. CR rats ran significantly more and reached a significantly higher breakpoint (i.e., last ratio not completed) than AL rats, indicating that calorie restriction increased both running performance and the motivation to run. A secondary objective was to explore whether steroids in the brain modulate the motivation to exercise. A panel of steroids including corticosterone, testosterone, and 17β-estradiol were measured in blood and microdissected brain regions after the end of the behavioural experiment using liquid chromatography tandem mass spectrometry (LC-MS/MS). Levels of testosterone and the less potent androgen androstenedione in the brain were altered by calorie restriction in a region-specific manner. Given that androgens have previously been shown to increase wheel running and calorie restriction increased the motivation to run in the current study, these results suggest that the motivation to exercise may be modulated by androgen signaling in the brain. To our knowledge, this study is the first to directly investigate the neuroendocrine mechanisms of the motivation to exercise. This work establishes a simple, specific, and effective operant task for assessing the motivation to exercise in rats and provides insight into potential neurosteroidal mechanisms modulating this behaviour.
title: Synthesis, and Structural and Spectroscopic Analysis of Trielyl-Derived Complexes of Iron
Creators: name: Griffin, Liam P.
name: Bauer, Alexis K.
name: Crumpton, Agamemnon E.
name: Ellwanger, Mathias A.
name: Heilmann, Andreas
name: Wiesner, Anja
name: Neidig, Michael L.
name: Aldridge, Simon
Publisher: name: Freie Universität Berlin
Year: 2025
Subjects: subject: aluminium
subject: gallium
subject: indium
subject: iron
subject: Mössbauer spectroscopy
classificationCode: 540
subject: Chemie und zugeordnete Wissenschaften

description: The reactivity of group 13 anions of the form [(NON)E]− (NON=4,5-bis(2,6-diisopropylanilido)-2,7-di-tert-butyl-9,9-dimethyl-xanthene, E=Al, Ga, In) towards Fe(CO)5 has been investigated. In the case of the aluminyl system, both reaction outcome and product structure are highly sensitive to the availability of the potassium counterion; sequestration by 18-crown-6 is necessary to yield a species featuring a direct, unsupported Al−Fe bond. 2.2.2-Cryptand, by contrast, yields a species featuring bridging carbonyl ligands, while the use of no sequestering agent at all leads to isocarbonyl bridging to aluminium. Owing to their lower oxophilicity, the heavier congeners gallium and indium more straightforwardly deliver Fe−E bonded adducts (E=Ga, In). The series of trielyl iron complexes has been interrogated by structural and computational analyses, as well as by IR and Mössbauer spectroscopies, revealing a consistent shift in bond polarity and electron richness at iron as group 13 is descended. This in turn is consistent with the diminishing donor strength of the trielyl ligand with increasing atomic number.
Additional file 2 of Grapevine (Vitis vinifera) responses to salt stress and alkali stress: transcriptional and metabolic profiling
Creators: name: Lu, Xu
name: Ma, Lei
name: Zhang, CongCong
name: Yan, HaoKai
name: Bao, JinYu
name: Gong, MeiShuang
name: Wang, WenHui
name: Li, Sheng
name: Ma, ShaoYing
name: Chen, BaiHong
Publisher: name: figshare
Year: 2022
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: Plant Biology

description: Additional file 2: Table S1. Primer sequences used in qRT-PCR analysis.
Map #37, Site 5MT10686, Arbitrary Unit 102, Segment 1 (Backhoe Trench), Stratigraphic Profile
Creators: name: DeVos, Jim
Year: 2019
Subjects: subject: Southwestern Colorado
valueUri: https://core.tdar.org/browse/geographic-keyword/2810/southwestern-colorado
subject: Southwestern US
valueUri: https://core.tdar.org/browse/geographic-keyword/84604/southwestern-us
subject: Pueblo II
valueUri: https://core.tdar.org/browse/temporal-keyword/55/pueblo-ii
subject: Pueblo III
valueUri: https://core.tdar.org/browse/temporal-keyword/56/pueblo-iii
subject: 5MT10686
valueUri: https://core.tdar.org/browse/site-name/118038/5mt10686
subject: Badger Den
valueUri: https://core.tdar.org/browse/site-name/118037/badger-den

description: Site 5MT10686, Arbitrary Unit 102, Segment 1 (Backhoe Trench), Stratigraphic Profile
title: Justice for earth dwellers: a reply to my critics
Creators: name: Huber, Jakob
Publisher: name: Freie Universität Berlin
Year: 2025
Subjects: subject: Kant
subject: cosmopolitanism
subject: territory
subject: global justice
subject: migration
subject: colonialism
classificationCode: 102
subject: Verschiedenes

description: In responding to the stimulating commentaries by Alice Pinheiro Walla, Anna Stilz and Elisabeth Ellis, I aim to clarify and stimulate further engagement with some of the main arguments in ‘Kant’s Grounded Cosmopolitanism’. In particular, I highlight two premises that are seminal to Kant’s systematic framework of political morality as I reconstruct it: the radically relational and political nature of rights. While the commentators articulate various lines of critique, many of their queries can be understood as targeting (one or both of) these tenets on exegetical and/or systematic grounds.