title: ÐÐ·ÑÑÐµÐ½Ð¸Ðµ ÑÐ¾Ð»Ð¸ ÑÐ°ÐºÑÐ¾ÑÐ° Ð¡HD1 Ð² ÑÐµÐ³ÑÐ»ÑÑÐ¸Ð¸ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Drosophila melanogaster
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: The study of CHD1 factor in the regulation of gene expression in Drosophila melanogaster
Creators: name: Ð ÐµÐ¿Ð¸Ð½ÑÐºÐ°Ñ, ÐÐ°Ð½Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÐÐ¸Ð¾ÑÐµÑ Ð½Ð¾Ð»Ð¾Ð³Ð¸Ñ
Ñ ÑÐ¾Ð¼Ð°ÑÐ¸Ð½-ÑÐµÐ¼Ð¾Ð´ÐµÐ»Ð¸ÑÑÑÑÐ¸Ðµ ÑÐ°ÐºÑÐ¾ÑÑ
ÑÐ¿Ð¸Ð³ÐµÐ½ÐµÑÐ¸ÐºÐ°
Ð±Ð¸Ð¾Ð¸Ð½ÑÐ¾ÑÐ¼Ð°ÑÐ¸ÐºÐ°
Ð°Ð½Ð°Ð»Ð¸Ð· Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð¹ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð²
chromatin-remodeling factors
epigenetics
bioinformatics
analysis of differentially expressed genes

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«ÐÐ·ÑÑÐµÐ½Ð¸Ðµ ÑÐ¾Ð»Ð¸ ÑÐ°ÐºÑÐ¾ÑÐ° Ð¡HD1 Ð² ÑÐµÐ³ÑÐ»ÑÑÐ¸Ð¸ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Drosophila melanogasterÂ». Ð¦ÐµÐ»Ñ ÐÐÐ : Ð¸Ð·ÑÑÐ¸ÑÑ Ð±Ð¸Ð¾Ð¸Ð½ÑÐ¾ÑÐ¼Ð°ÑÐ¸ÑÐµÑÐºÐ¸Ð¼Ð¸ Ð¼ÐµÑÐ¾Ð´Ð°Ð¼Ð¸ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ ÑÐ°ÐºÑÐ¾ÑÐ° ÑÐ±Ð¾ÑÐºÐ¸ Ð¸ ÑÐµÐ¼Ð¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÑÑÐ¾Ð¼Ð°ÑÐ¸Ð½Ð° CHD1 Ð½Ð° Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ Ð¿ÑÐ¾ÑÐ¸Ð»ÐµÐ¹ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Drosophila melanogaster. ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: 1. ÐÐ·ÑÑÐ¸ÑÑ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÑ Ð² ÑÑÐµÑÐ°Ñ ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¸ Ð³ÐµÐ½ÐµÑÐ¸ÐºÐ¸ Ð²Ð¸Ð´Ð° Drosophila melanogaster; 2. ÐÑÑÐ°Ð±Ð¾ÑÐ°ÑÑ ÑÐ°Ð³Ð¸ Ð±Ð¸Ð¾Ð¸Ð½ÑÐ¾ÑÐ¼Ð°ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¿Ð°Ð¹Ð¿Ð»Ð°Ð¹Ð½Ð°; 3. ÐÐ¸Ð·ÑÐ°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°ÑÑ Ð´Ð°Ð½Ð½ÑÐµ Ð² ÑÑÐµÐ´Ðµ RStudio; 4. ÐÐ½ÑÐµÑÐ¿ÑÐµÑÐ¸ÑÐ¾Ð²Ð°ÑÑ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð¸ ÑÐ´ÐµÐ»Ð°ÑÑ Ð²ÑÐ²Ð¾Ð´ Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ð¸ ÑÐ°ÐºÑÐ¾ÑÐ° CHD1 Ð½Ð° ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð³ÐµÐ½Ð¾Ð² Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð¾Ð² Ð²Ð¸Ð´Ð° Drosophila melanogaster. ÐÐ±ÑÐµÐºÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â Drosophila melanogaster, Ð¿ÑÐµÐ´Ð¼ÐµÑÐ¾Ð¼ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐ²Ð»ÑÐµÑÑÑ ÑÐµÐ³ÑÐ»ÑÑÐ¸Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² ÑÐ°ÐºÑÐ¾ÑÐ¾Ð¼ CHD1. Ð¤ÑÐ½ÐºÑÐ¸Ð¸ Ð±ÐµÐ»ÐºÐ° Ð¸Ð·ÑÑÐ°ÑÑÑÑ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð´Ð¾Ð·Ð¾Ð²Ð¾Ð¹ ÐºÐ¾Ð¼Ð¿ÐµÐ½ÑÐ°ÑÐ¸Ð¸ Ð¸ Ð±Ð¸Ð¾Ð¸Ð½ÑÐ¾ÑÐ¼Ð°ÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÐ¸ Ð´Ð°Ð½Ð½ÑÑ Ð ÐÐ-ÑÐµÐºÐ²ÐµÐ½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ. ÐÐ½ÑÐµÑÐ¿ÑÐµÑÐ°ÑÐ¸Ñ Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»Ð°ÑÑ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð±Ð°Ð· Ð´Ð°Ð½Ð½ÑÑ NCBI Gene Ð¸ FlyBase. Ð Ð°Ð±Ð¾ÑÐ° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ ÐÐÐ¦ Â«ÐÑÑÑÐ°ÑÐ¾Ð²ÑÐºÐ¸Ð¹ Ð¸Ð½ÑÑÐ¸ÑÑÑÂ» â ÐÐÐ¯Ð¤. Ð ÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð¿Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÑÑ, ÑÑÐ¾ Ñ ÑÐ°Ð¼ÑÐ¾Ð² Ñ Ð¼ÑÑÐ°ÑÐ¸ÐµÐ¹ Chd1 Ð°ÐºÑÐ¸Ð²Ð½ÐµÐµ ÑÐºÑÐ¿ÑÐµÑÑÐ¸ÑÑÑÑÑÑ Ð³ÐµÐ½Ñ Ñ Ð¥-ÑÑÐ¾Ð¼Ð¾ÑÐ¾Ð¼Ñ. Ð¢Ð°ÐºÐ¶Ðµ Ð² ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ð¸ Ñ Ð¾Ð±ÑÐ°Ð·ÑÐ°Ð¼Ð¸ Ð´Ð¸ÐºÐ¾Ð³Ð¾ ÑÐ¸Ð¿Ð° Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¾ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Ð³Ð¸ÑÑÐ¾Ð½Ð¾Ð²ÑÑ Ð±ÐµÐ»ÐºÐ¾Ð² Ð¸ Ð³ÐµÐ½Ð¾Ð² ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ ÑÑÑÐµÑÑÐ°. ÐÐ±Ð»Ð°ÑÑÑ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð° Ð²ÐºÐ»Ð°Ð´Ð¾Ð¼ ÑÐ°Ð±Ð¾ÑÑ Ð² Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð² ÑÐµÐ¼Ð¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÑÑÐ¾Ð¼Ð°ÑÐ¸Ð½Ð° Ð¸ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾ÑÑÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð² Ð² Ð±Ð¾ÑÑÐ±Ðµ Ñ Ð¾Ð½ÐºÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ð¼Ð¸ Ð¸ Ð½ÐµÐ¹ÑÐ¾Ð´ÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸Ð²Ð½ÑÐ¼Ð¸ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸ÑÐ¼Ð¸.
description: The subject of the graduate qualification work is âThe study ofÂ  CHD1 factor in the regulation of gene expression in Drosophila melanogasterâ. The given work is devoted to the research of the effect of chromatin assembly and remodeling factor CHD1 on the change of gene expression profiles in Drosophila melanogaster with bioinformatics. The research sets the following goals: 1. Review the literature in the fields of physiology and genetics of Drosophila melanogaster; 2. Master the steps of the bioinformatical pipeline; 3. Visualize acquired data in RStudio IDE; 4. Interpret the results and conclude the effect of the CHD1 factor on gene expression in Drosophila melanogaster. The object of our research is Drosophila melanogaster species. The subject is the regulation of gene expression with CHD1 factor. The functions of this protein are studied with a dosage compensation model and the bioinformatical methods of RNA-sequencing data processing. The interpretation was performed with the help of NCBI Gene and FlyBase databases. The work was fulfilled on the premises of NRC Â«Kurchatov InstituteÂ» - PNPI. The results show that the genes from X-chromosome are expressed stronger in males with Chd1 mutation. In comparison with the âwild typeâ samples, the change in expression of the histone genes and the genes of stress response was seen. The field of application is defined by the contribution of this work to the overall research of chromatin remodeling factors and the opportunity of using the results to address oncological and neurodegenerative diseases.