Plasmid maps for constructs generated in 'Multiplexed effector screening for recognition by endogenous resistance genes using positive defense reporters in wheat protoplasts'
Creators: name: Salome Wilson
name: Bayantes Dagvadorj
name: Rita Tam
name: Lydia Murphy
name: Shulz-Kroenert, Sven
name: Nigel Heng
name: Emma Crean
name: John P. Rathjen
name: Benjamin Schwessinger
Publisher: name: Zenodo
Year: 2023
Subjects: plasmid maps

description: Plasmid maps:



	
		
			Plasmid Name
			Insert
			Backbone
			PROM
			CDS
			Term
			GG level
			GG Part
			Bacterial Resistance
			Source
			Addgene ID
			Plasmid Map Name
		
		
			From other Sources
			 
			 
			 
			 
			 
			 
			 
			 
			 
			 
			 
		
		
			pUPD2+E-Luc
			E-Luc green luciferase
			pUPD2
			N/A
			N/A
			N/A
			0
			CDS
			Chloramphenicol
			Addgene
			170873
			N/A
		
		
			pRedF
			GBPart - RedF Luciferase CDS
			pUPD
			N/A
			N/A
			N/A
			0
			CDS
			Ampicillin
			Addgene
			118057
			N/A
		
		
			pICSL12009
			Zea mays lvl 0 Promoter
			pICH41295
			N/A
			N/A
			N/A
			0
			PROM
			Spectinomycin
			Addgene
			68257
			N/A
		
		
			pICSL80001
			fLuc lvl 0 CDS
			pICH41308
			N/A
			N/A
			N/A
			0
			CDS
			Spectinomycin
			Addgene
			50326
			N/A
		
		
			pICH41421
			NosT lvl 0 Terminator
			pICH41276
			N/A
			N/A
			N/A
			0
			TERM 
			Spectinomycin
			Addgene
			50339
			N/A
		
		
			pAGM9121
			Universal lvl 0 acceptor (from kit)
			pUC19 derived
			N/A
			N/A
			N/A
			0
			Backbone
			Spectinomycin
			Addgene
			51833
			N/A
		
		
			pICH47732
			lvl1 acceptor (forward position 1)
			derived from pBIN19 and pUC19
			N/A
			N/A
			N/A
			0
			Backbone
			Ampicillin
			Addgene
			48000
			N/A
		
		
			pICH47742
			lvl1 acceptor (forward position 2)
			derived from pBIN19 and pUC19
			N/A
			N/A
			N/A
			0
			Backbone
			Ampicillin
			Addgene
			48001
			N/A
		
		
			pUBI-AvrSr35
			expression under UBI promoter, Amp R
			N/A
			N/A
			N/A
			N/A
			1
			L1 expression
			Ampicillin
			shared by CSIRO 
			N/A
			N/A
		
		
			pUBI-Sr35
			expression under UBI promoter, Amp R
			N/A
			N/A
			N/A
			N/A
			1
			L1 expression
			Ampicillin
			shared by CSIRO 
			N/A
			N/A
		
		
			pUBI-AvrSr50
			expression under UBI promoter, Amp R
			N/A
			N/A
			N/A
			N/A
			1
			L1 expression
			Ampicillin
			shared by CSIRO 
			N/A
			N/A
		
		
			pUBI-Sr50
			expression under UBI promoter, Amp R
			N/A
			N/A
			N/A
			N/A
			1
			L1 expression
			Ampicillin
			shared by CSIRO 
			N/A
			N/A
		
		
			Level 0 plasmids
			 
			 
			 
			 
			 
			 
			 
			 
			 
			 
			 
		
		
			PROM_1
			D1: Promoter of TraesCS1A02G332800 
			Twist Bioscience Chloramphenicol vector
			N/A
			N/A
			N/A
			0
			PROM
			Chloramphenicol
			Schwessinger Lab
			201294
			PROM_1.gb
		
		
			PROM_2
			D2: Promoter of TraesCS2B02G533000
			Twist Bioscience Chloramphenicol vector
			N/A
			N/A
			N/A
			0
			PROM
			Chloramphenicol
			Schwessinger Lab
			201295
			PROM_2.gb
		
		
			PROM_3
			D3: Promoter of TraesCS3A02G510700
			Twist Bioscience Chloramphenicol vector
			N/A
			N/A
			N/A
			0
			PROM
			Chloramphenicol
			Schwessinger Lab
			201296
			PROM_3.gb
		
		
			PROM_4
			D4: Promoter of TraesCS3B02G552700
			Twist Bioscience Chloramphenicol vector
			N/A
			N/A
			N/A
			0
			PROM
			Chloramphenicol
			Schwessinger Lab
			201297
			PROM_4.gb
		
		
			PROM_5
			D5: Promoter of TraesCS3B02G553900
			Twist Bioscience Chloramphenicol vector
			N/A
			N/A
			N/A
			0
			PROM
			Chloramphenicol
			Schwessinger Lab
			201298
			PROM_5.gb
		
		
			PROM_6
			D6: Promoter of TraesCS3B02G578800
			Twist Bioscience Chloramphenicol vector
			N/A
			N/A
			N/A
			0
			PROM
			Chloramphenicol
			Schwessinger Lab
			201299
			PROM_6.gb
		
		
			PROM_7
			D7: Promoter of TraesCS3D02G316100
			Twist Bioscience Chloramphenicol vector
			N/A
			N/A
			N/A
			0
			PROM
			Chloramphenicol
			Schwessinger Lab
			201300
			PROM_7.gb
		
		
			PROM_10
			D10: Promoter of TraesCS3D02G517600
			Twist Bioscience Chloramphenicol vector
			N/A
			N/A
			N/A
			0
			PROM
			Chloramphenicol
			Schwessinger Lab
			201301
			PROM_10.gb
		
		
			PROM_11
			D11: Promoter of TraesCS4A02G235900
			Twist Bioscience Chloramphenicol vector
			N/A
			N/A
			N/A
			0
			PROM
			Chloramphenicol
			Schwessinger Lab
			201302
			PROM_11.gb
		
		
			PROM_12
			D12: Promoter of TraesCS4D02G131100
			Twist Bioscience Chloramphenicol vector
			N/A
			N/A
			N/A
			0
			PROM
			Chloramphenicol
			Schwessinger Lab
			201303
			PROM_12.gb
		
		
			pBD004
			D14: Promoter of TraesCS3D02G368800
			pAGM9121
			N/A
			N/A
			N/A
			0
			PROM
			Spectinomycin
			Schwessinger Lab
			201304
			pBD004.gb
		
		
			pBD005
			D15: Promoter of TraesCS3D02G517500
			pAGM9121
			N/A
			N/A
			N/A
			0
			PROM
			Spectinomycin
			Schwessinger Lab
			201305
			pBD005.gb
		
		
			pBD006
			D16: Promoter of TraesCS5D02G320700
			pAGM9121
			N/A
			N/A
			N/A
			0
			PROM
			Spectinomycin
			Schwessinger Lab
			201306
			pBD006.gb
		
		
			pBD016
			Sr27
			pAGM9121
			N/A
			N/A
			N/A
			0
			CDS
			Spectinomycin
			Schwessinger Lab
			201307
			pBD016.gb
		
		
			pBD025
			AvrSr27-1
			Twist Chloramphenicol vector
			N/A
			N/A
			N/A
			0
			CDS
			Chloramphenicol
			Schwessinger Lab
			201308
			pBD025.gb
		
		
			pBD026
			AvrSr27-2
			Twist Chloramphenicol vector
			N/A
			N/A
			N/A
			0
			CDS
			Chloramphenicol
			Schwessinger Lab
			201309
			pBD026.gb
		
		
			pBD027
			avrSr27-3
			Twist Chloramphenicol vector
			N/A
			N/A
			N/A
			0
			CDS
			Chloramphenicol
			Schwessinger Lab
			201310
			pBD27.gb
		
		
			pLM001
			AvrSr50 (with stop codon)
			pAGM9121
			N/A
			N/A
			N/A
			0
			CDS
			Spectinomycin
			Schwessinger Lab
			201311
			pLM001.gb
		
		
			pLM002
			AvrSr50 (no stop codon)
			pAGM9121
			N/A
			N/A
			N/A
			0
			CDS
			Spectinomycin
			Schwessinger Lab
			201312
			pLM002.gb
		
		
			pLM003
			AvrSr35 (with stop codon)
			pAGM9121
			N/A
			N/A
			N/A
			0
			CDS
			Spectinomycin
			Schwessinger Lab
			201313
			pLM003.gb
		
		
			pLM004
			AvrSr35 (no stop codon)
			pAGM9121
			N/A
			N/A
			N/A
			0
			CDS
			Spectinomycin
			Schwessinger Lab
			201314
			pLM004.gb
		
		
			Level 1 plasmids
			 
			 
			 
			 
			 
			 
			 
			 
			 
			 
			 
		
		
			pSW027
			UBI:fLUC
			pICH47802
			pICSL12009
			pICSL80001
			pICH41421
			1
			L1 P1 (reverse orientation)
			Ampicillin
			Schwessinger Lab
			201315
			pSW027.gb
		
		
			pSW029
			UBI:pRedF
			pICSL47732
			pICSL12009
			pRedF
			pICH41421
			1
			L1 P1
			Ampicillin
			Schwessinger Lab
			201316
			pSW029.gb
		
		
			pSW030
			UBI:Eluc
			pICSL47732
			pICSL12009
			pUPD2+E-Luc
			pICH41421
			1
			L1 P1
			Ampicillin
			Schwessinger Lab
			201317
			pSW030.gb
		
		
			pBD024
			UBI:Sr27
			pICH47732
			pICSL12009
			pBD016
			pICH41421
			1
			L1 P1
			Ampicillin
			Schwessinger Lab
			201318
			pBD024.gb
		
		
			pBD030
			UBI:AvrSr27-1
			pICH47742
			pICSL12009
			pBD025
			pICH41421
			1
			L1 P1
			Ampicillin
			Schwessinger Lab
			201319
			pBD030.gb
		
		
			pSW001
			pDefence1:Eluc
			pICH47732
			PROM_1
			pUPD2+E-Luc
			pICH41421
			1
			L1 P1
			Ampicillin
			Schwessinger Lab
			201320
			pSW001.gb
		
		
			pSW002
			pDefence2:Eluc
			pICH47732
			PROM_2
			pUPD2+E-Luc
			pICH41421
			1
			L1 P1
			Ampicillin
			Schwessinger Lab
			201321
			pSW002.gb
		
		
			pSW003
			pDefence3:Eluc
			pICH47732
			PROM_3
			pUPD2+E-Luc
			pICH41421
			1
			L1 P1
			Ampicillin
			Schwessinger Lab
			201322
			pSW003.gb
		
		
			pSW004
			pDefence4:Eluc
			pICH47732
			PROM_4
			pUPD2+E-Luc
			pICH41421
			1
			L1 P1
			Ampicillin
			Schwessinger Lab
			201323
			pSW004.gb
		
		
			pSW005
			pDefence5:Eluc
			pICH47732
			PROM_5
			pUPD2+E-Luc
			pICH41421
			1
			L1 P1
			Ampicillin
			Schwessinger Lab
			201324
			pSW005.gb
		
		
			pSW006
			pDefence6:Eluc
			pICH47732
			PROM_6
			pUPD2+E-Luc
			pICH41421
			1
			L1 P1
			Ampicillin
			Schwessinger Lab
			201325
			pSW006.gb
		
		
			pSW007
			pDefence7:Eluc
			pICH47732
			PROM_7
			pUPD2+E-Luc
			pICH41421
			1
			L1 P1
			Ampicillin
			Schwessinger Lab
			201326
			pSW007.gb
		
		
			pSW010
			pDefence10:Eluc
			pICH47732
			PROM_10
			pUPD2+E-Luc
			pICH41421
			1
			L1 P1
			Ampicillin
			Schwessinger Lab
			201327
			pSW010.gb
		
		
			pSW011
			pDefence11:Eluc
			pICH47732
			PROM_11
			pUPD2+E-Luc
			pICH41421
			1
			L1 P1
			Ampicillin
			Schwessinger Lab
			201328
			pSW011.gb
		
		
			pSW012
			pDefence12:Eluc
			pICH47732
			PROM_12
			pUPD2+E-Luc
			pICH41421
			1
			L1 P1
			Ampicillin
			Schwessinger Lab
			201329
			pSW012.gb
		
		
			pBD001
			pDefence14:Eluc
			pICH47732
			pBD004
			pUPD2+E-Luc
			pICH41421
			1
			L1 P1
			Ampicillin
			Schwessinger Lab
			201330
			pBD001.gb
		
		
			pBD002
			pDefence15:Eluc
			pICH47732
			pBD005
			pUPD2+E-Luc
			pICH41421
			1
			L1 P1
			Ampicillin
			Schwessinger Lab
			201331
			pBD002.gb
		
		
			pBD003
			pDefence16:Eluc
			pICH47732
			pBD006
			pUPD2+E-Luc
			pICH41421
			1
			L1 P1
			Ampicillin
			Schwessinger Lab
			201332
			pBD003.gb