Additional file 1 of PtoNF-YC9-SRMT-PtoRD26 module regulates the high saline tolerance of a triploid poplar
Creators: name: Tong, Shaofei
name: Wang, Yubo
name: Chen, Ningning
name: Wang, Deyan
name: Liu, Bao
name: Wang, Weiwei
name: Chen, Yang
name: Liu, Jianquan
name: Ma, Tao
name: Jiang, Yuanzhong
Publisher: name: figshare
Year: 2022
Subjects: subject: Genetics
subject: FOS: Biological sciences

description: Additional file 1. Figure S1. Flow cytometry estimation of the P. × tomentosa Carr. clone 741 genome size. Figure S2. The sequence identity of CDS (A), proteins (B) and 1.5 kb promoter sequences (C) among different haplotypes. Figure S3. Heatmap indicating the expression levels of differentially expressed genes (DEGs) from the RNA-seq of the salt-stressed triploid poplars. Figure S4. The tissue expression pattern of PtoRD26 in this triploid poplar. Figure S5. Determination of PtoRD26 expression level in the PtoRD26.1 overexpression and RNAi lines. Figure S6. The sequence alignments of three PtoRD26 alleles in the triploid poplars and their ortholog in P. alba var. pyramidalis. Figure S7. The phylogenetic relationship and peptide sequence of SRMT. Figure S8. Tissue expression pattern analysis of SRMT in the triploid Chinese white poplars. Figure S9. Both PtoRD26.2 and PtoRD26.3 upregulated the expression of LUC driven by SRMT.3pro promoter in tobacco leaves. Figure S10. The sequence alignments of two SRMT alleles in the triploid poplars and their ortholog in P. alba var. pyramidalis. Figure S11. The distributions of PtoRD26 binding elements in the promoters of SRMT alleles in this triploid poplar and in the promoter of the ortholog in P. alba var. pyramidalis. Figure S12. Identification of morphology of SRMT transgenic poplars. Figure S13. Sensitivity of SRMT.3-OE, SRMT-RNAi and WT poplar cuttings to ABA treatment. Figure S14. Both SRMT.2 and SRMT.3 upregulated the expression of LUC driven by PtoRD26.1 promoter in tobacco leaves. Figure S15. EMSA indicated that the binding sites of SRMT.3 in the 1.5 kb promoter region of PtoRD26.1. Figure S16. The distributions of SRMT binding elements in the promoters of PtoRD26 alleles in this triploid poplar and in the promoter of the ortholog in P. alba var. pyramidalis. Figure S17. The determination of the PtoRD26 expression level in the double transgenic poplars (PtoRD26-RNAi/SRMT.3-OE). Figure S18. The temporal expression pattern of PtoRD26 in response to MeJA was independent on SRMT. Figure S19. Self-activation activity identification of SRMT.3 in yeast strain AH109. Figure S20. The peptide sequence of NF-YC9 from various species and the spatio-temporal expression pattern of PtoNF-YC9.1. Figure S21. The expression levels of PtoNF-YC9 in the PtoNF-YC9.1 overexpression and RNAi poplars by qPCR. Figure S22. The determination of the SRMT expression level in the double knocking-down poplars (SRMT-RNAi/PtoNF-YC9-RNAi). Figure S23. The sequence alignments of three PtoNF-YC9 alleles in the triploid poplars and their ortholog in P. alba var. pyramidalis. Figure S24. SRMT and PtoNF-YC9 alleles were synergistically upregulated the expression of LUC driven by PtoRD26.1 promoter in tobacco leaves. Figure S25. The plant height and stem diameter of all transgenic lines in this study.