title: ÐÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ðµ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ ÑÑÐµÑÐ¾Ð¸Ð´Ð¾Ð² Ð´Ð»Ñ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ¸ Ð¼Ð¾Ð´ÐµÐ»Ð¸ ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ñ in vitro
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Using cell spheroids to develop an in vitro model of the endometrium
Creators: name: ÐÐ°ÑÑÐ¾Ð½Ð¾Ð²Ð°, ÐÐ»Ð¸Ð·Ð°Ð²ÐµÑÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐµ ÑÑÐµÑÐ¾Ð¸Ð´Ñ
ÑÐÐ¡Ð
3D-ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
Ð¼Ð¾Ð´ÐµÐ»Ñ ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ñ
Ð´ÐµÑÐ¸Ð´ÑÐ°Ð»ÑÐ½Ð°Ñ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐ°
Ð¼Ð¾Ð´ÐµÐ»Ñ ÑÐºÐ°Ð½Ð¸ in vitro
cell spheroids
eMSC
3D-cultivation
model of endometrium
decidual differentiation
model of endometrium in vitro

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ ÑÑÐµÑÐ¾Ð¸Ð´Ð¾Ð², ÑÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ Ð¸Ð· ÑÐÐ¡Ð, Ð½Ð° Ð¿ÑÐµÐ´Ð¼ÐµÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ñ Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð¼Ð¾Ð´ÐµÐ»Ð¸ ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ñ. Ð­Ð½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ð¹ â Ð²Ð½ÑÑÑÐµÐ½Ð½ÑÑ Ð¾Ð±Ð¾Ð»Ð¾ÑÐºÐ° Ð¼Ð°ÑÐºÐ¸, ÐºÐ¾ÑÐ¾ÑÐ°Ñ Ð¾Ð±ÐµÑÐ¿ÐµÑÐ¸Ð²Ð°ÐµÑ Ð½Ð°ÑÑÑÐ¿Ð»ÐµÐ½Ð¸Ðµ Ð¸ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ðµ Ð±ÐµÑÐµÐ¼ÐµÐ½Ð½Ð¾ÑÑÐ¸. ÐÑÑÑÐµÐµ Ð¿Ð¾Ð½Ð¸Ð¼Ð°Ð½Ð¸Ðµ ÑÑÐ¸Ñ Ð¿ÑÐ¾ÑÐµÑÑÐ¾Ð², Ð° ÑÐ°ÐºÐ¶Ðµ Ð¿Ð°ÑÐ¾Ð»Ð¾Ð³Ð¸Ð¹ ÑÐµÐ¿ÑÐ¾Ð´ÑÐºÑÐ¸Ð²Ð½Ð¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ñ, Ð¿Ð¾Ð·Ð²Ð¾Ð»Ð¸Ñ ÑÐ°Ð·ÑÐ°Ð±Ð°ÑÑÐ²Ð°ÑÑ Ð½Ð¾Ð²ÑÐµ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÑ Ð¸ Ð¿Ð¾Ð´ÑÐ¾Ð´Ñ Ðº Ð»ÐµÑÐµÐ½Ð¸Ñ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ð¹ Ð¸ ÑÐµÑÐ¸ÑÑ Ð°ÐºÑÑÐ°Ð»ÑÐ½ÑÑ Ð¿ÑÐ¾Ð±Ð»ÐµÐ¼Ñ Ð¶ÐµÐ½ÑÐºÐ¾Ð³Ð¾ Ð±ÐµÑÐ¿Ð»Ð¾Ð´Ð¸Ñ. Ð¡ÑÑÐµÑÑÐ²ÑÑÑÐ¸Ðµ Ð² Ð½Ð°ÑÑÐ¾ÑÑÐ¸Ð¹ Ð¼Ð¾Ð¼ÐµÐ½Ñ in vitro Ð¸ in vivo Ð¼Ð¾Ð´ÐµÐ»Ð¸ ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ñ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð½Ðµ Ð¿Ð¾Ð»Ð½Ð¾ÑÑÑÑ ÑÐ´Ð¾Ð²Ð»ÐµÑÐ²Ð¾ÑÑÑÑ ÐµÐ³Ð¾ ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð°Ð»ÑÐ½ÑÐ¼ Ð¸Ð»Ð¸ ÑÑÑÑÐºÑÑÑÐ½ÑÐ¼ Ð¾ÑÐ¾Ð±ÐµÐ½Ð½Ð¾ÑÑÑÐ¼. ÐÐ¾ÑÑÐ¾Ð¼Ñ Ð² Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐµ ÑÑÐµÑÐ¾Ð¸Ð´Ñ Ð¸Ð· ÑÐÐ¡Ð, ÑÐ´Ð¾Ð²Ð»ÐµÑÐ²Ð¾ÑÑÑÑÐ¸Ðµ ÑÑÑÑÐºÑÑÑÐ½ÑÐ¼ Ð¾ÑÐ¾Ð±ÐµÐ½Ð½Ð¾ÑÑÑÐ¼ ÑÐºÐ°Ð½Ð¸, Ð½Ð° Ð¿ÑÐµÐ´Ð¼ÐµÑ ÑÐ°ÐºÐ¶Ðµ ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÑÐ¾Ð´ÑÑÐ²Ð° Ñ ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ð¼Ð¸ Ð¾ÑÐ¾Ð±ÐµÐ½Ð½Ð¾ÑÑÑÐ¼Ð¸ ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ñ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°. ÐÐ·Ð³Ð¾ÑÐ¾Ð²Ð»ÐµÐ½Ð½ÑÐµ ÑÑÐµÑÐ¾Ð¸Ð´Ñ Ð¿ÑÐ¾Ð²ÐµÑÑÐ»Ð¸ Ð½Ð° ÑÐ¾ÑÑÐ°Ð½ÐµÐ½Ð¸Ðµ Ð¶Ð¸Ð·Ð½ÐµÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÐ¸ Ð¸ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÐ¸ Ðº ÑÐºÐ°Ð½ÐµÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐ½Ð¾Ð¹ Ð´ÐµÑÐ¸Ð´ÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐµ, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¿ÑÐ¾Ð²ÐµÑÑÐ»Ð¸ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð³ÐµÐ½Ð¾Ð²-Ð¼Ð°ÑÐºÐµÑÐ¾Ð² Ð´ÐµÑÐ¸Ð´ÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐ¸ PRL Ð¸ IGFBP-1, Ð°Ð½Ð³Ð¸Ð¾Ð³ÐµÐ½Ð½ÑÑ (HGF Ð¸ VEGF) Ð¸ Ð¿ÑÐ¾ÑÐ¸Ð²Ð¾Ð²Ð¾ÑÐ¿Ð°Ð»Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ (TSG-6) ÑÐ°ÐºÑÐ¾ÑÐ¾Ð². Ð ÑÐ¾Ð´Ðµ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð¿ÑÐ¸ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ Ð² ÑÑÐµÑÐ¾Ð¸Ð´Ð°Ñ ÑÐÐ¡Ð ÑÐ¾ÑÑÐ°Ð½ÑÑÑ Ð¶Ð¸Ð·Ð½ÐµÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ Ð¸ ÑÐµÐºÑÐµÑÐ¸ÑÑÑÑ Ð¿ÑÐ¾Ð»Ð°ÐºÑÐ¸Ð½, Ð±ÐµÐ»Ð¾Ðº-Ð¼Ð°ÑÐºÐµÑ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐ¸, Ð² Ð¾ÑÐ²ÐµÑ Ð½Ð° Ð³Ð¾ÑÐ¼Ð¾Ð½Ð°Ð»ÑÐ½ÑÑ ÑÑÐ¸Ð¼ÑÐ»ÑÑÐ¸Ñ. Ð¢Ð°ÐºÐ¶Ðµ Ð±ÑÐ»Ð¾ Ð¿ÑÐ¾Ð´ÐµÐ¼Ð¾Ð½ÑÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¾, ÑÑÐ¾ Ð¾ÑÐ½Ð¾ÑÐ¸ÑÐµÐ»ÑÐ½Ð°Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ ÐºÐ°Ðº Ð³ÐµÐ½Ð¾Ð²-Ð¼Ð°ÑÐºÐµÑÐ¾Ð² Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐ¸, ÑÐ°Ðº Ð¸ Ð°Ð½Ð³Ð¸Ð¾Ð³ÐµÐ½Ð½ÑÑ Ð¸ Ð¿ÑÐ¾ÑÐ¸Ð²Ð¾Ð²Ð¾ÑÐ¿Ð°Ð»Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð² Ð²ÑÑÐµ Ð¿ÑÐ¸ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ Ð² ÑÑÐµÑÐ¾Ð¸Ð´Ð°Ñ, Ð² ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ð¸ Ñ ÐºÐ»ÐµÑÐºÐ°Ð¼Ð¸, ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÑÐµÐ¼ÑÐ¼Ð¸ Ð² Ð¼Ð¾Ð½Ð¾ÑÐ»Ð¾Ðµ. Ð¢Ð°ÐºÐ¸Ð¼ Ð¾Ð±ÑÐ°Ð·Ð¾Ð¼, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð¼Ð¾Ð´ÐµÐ»Ñ Ð¼Ð¾Ð¶Ð½Ð¾ ÑÐ°ÑÑÐ¼Ð°ÑÑÐ¸Ð²Ð°ÑÑ Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ ÑÐ»ÐµÐ´ÑÑÑÐµÐ³Ð¾ Ð¿ÑÐ¸Ð±Ð»Ð¸Ð¶ÐµÐ½Ð¸Ñ Ðº Ð¼Ð¾Ð´ÐµÐ»Ð¸ ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ñ, Ð¾ÑÐ½Ð¾Ð²ÑÐ²Ð°ÑÑÑÑ Ð½Ð° Ð½ÐµÐ¹ Ð´Ð»Ñ Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐ¸Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ Ð¸ ÑÐ»ÑÑÑÐµÐ½Ð¸Ð¹, ÑÑÐ¾Ð±Ñ ÑÐ¾Ð·Ð´Ð°ÑÑ ÐµÑÐµ Ð±Ð¾Ð»ÐµÐµ Ð±Ð»Ð¸Ð·ÐºÑÑ Ðº ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ð¼ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð°Ð¼ Ð¼Ð¾Ð´ÐµÐ»Ñ ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ñ in vitro.
description: This work is devoted to the study of cell spheroids formed from eMSCs for use as a model of the endometrium. Endometrium - the inner layer of the uterus, which ensures the onset and development of pregnancy. A better understanding of these processes, as well as the pathologies of the reproductive system, will allow the development of new drugs and approaches to the treatment of various diseases and solve the urgent problem of female infertility. The currently existing in vitro and in vivo models of the human endometrium do not fully satisfy its functional or structural features. Therefore, in this work, cell spheroids from eMSCs, which satisfy the structural features of the tissue, were also studied for functional similarity with the physiological features of the human endometrium. The fabricated spheroids were tested for viability and ability for tissuespecific decidual differentiation, as well as the expression of genes-markers of decidual differentiation PRL and IGFBP-1, angiogenic (HGF and VEGF) and anti-inflammatory (TSG-6) factors. In the course of the work, it was shown that when cultured in spheroids, eMSCs remain viable (about 88% of living cells) and secrete prolactin, a differentiation marker protein, in response to hormonal stimulation. It was also demonstrated that the relative expression of both differentiation marker genes and angiogenic and anti-inflammatory factors is higher when cultured in spheroids compared to cells cultured in a monolayer. Thus, the resulting model can be considered as the next approximation to the endometrial model, based on it for further research and improvements to create an in vitro model of the endometrium even closer to the physiological properties.