title: ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¿ÑÐ¾ÑÐ¸Ð»Ñ ÑÐ°ÑÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¸ Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐ¸ Ð±Ð¸Ð¾Ð´ÐµÐ³ÑÐ°Ð´Ð°ÑÐ¸Ð¸ Ð¿Ð¾Ð»Ð¸ÑÐ»ÐµÐºÑÑÐ¾Ð»Ð¸ÑÐ½ÑÑ Ð½Ð¾ÑÐ¸ÑÐµÐ»ÐµÐ¹ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸ Ð°ÐºÑÐ¸Ð²Ð½ÑÑ Ð²ÐµÑÐµÑÑÐ² in vivo
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Investigation of the distribution profile and dynamics of biodegradation of polyelectrolyte carriers of biologically active substances in vivo
Creators: name: Ð Ð¾Ð³Ð¾Ð²Ð°, ÐÐ½Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: ÑÐ°ÑÑÐ¸ÑÑ CaCO3
Ð¼Ð¸ÐºÑÐ¾ÑÐ°ÑÑÐ¸ÑÑ
Layer-by-Layer
Ð±Ð¸Ð¾ÑÐ°ÑÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ
Ð³Ð¸ÑÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ
Ð²Ð¸Ð·ÑÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ñ in vivo
CaCO3 particles
microparticles
drug delivery
biodistribution
histological examination
in vivo visualization

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¿ÑÐ¾ÑÐ¸Ð»Ñ ÑÐ°ÑÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¸ Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐ¸ Ð±Ð¸Ð¾Ð´ÐµÐ³ÑÐ°Ð´Ð°ÑÐ¸Ð¸ Ð¿Ð¾Ð»Ð¸ÑÐ»ÐµÐºÑÑÐ¾Ð»Ð¸ÑÐ½ÑÑ Ð½Ð¾ÑÐ¸ÑÐµÐ»ÐµÐ¹ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸ Ð°ÐºÑÐ¸Ð²Ð½ÑÑ Ð²ÐµÑÐµÑÑÐ² in vivoÂ». ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð¿ÑÐ¾ÑÐ¸Ð»Ñ Ð±Ð¸Ð¾ÑÐ°ÑÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¼Ð¸ÐºÑÐ¾Ð½Ð½ÑÑ ÑÐ°ÑÑÐ¸Ñ, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð¿Ð¾ ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¸ Layer-by-Layer, Ð³Ð´Ðµ Ð½Ð° ÑÐ´ÑÐ¾ Ð¸Ð· ÐºÐ°ÑÐ±Ð¾Ð½Ð°ÑÐ° ÐºÐ°Ð»ÑÑÐ¸Ñ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ Ð½Ð°Ð½Ð¾ÑÐ¸Ð»Ð¸ ÑÐ»Ð¾Ð¸ Ð¿Ð¾Ð»Ð¸Ð°ÑÐ³Ð¸Ð½Ð¸Ð½Ð° Ð¸ Ð´ÐµÐºÑÑÑÐ°Ð½ ÑÑÐ»ÑÑÐ°ÑÐ°. ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»Ð¸ÑÑ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð²Ð¸Ð·ÑÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸, ÑÐ°ÐºÐ¸Ñ ÐºÐ°Ðº ÑÐ²ÐµÑÐ¾Ð²Ð°Ñ Ð¸ ÐºÐ¾Ð½ÑÐ¾ÐºÐ°Ð»ÑÐ½Ð°Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ (Ð´Ð»Ñ Ð³Ð¸ÑÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ ÑÑÐµÐ·Ð¾Ð²), Ð° ÑÐ°ÐºÐ¶Ðµ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð°Ñ in vivo Ð²Ð¸Ð·ÑÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ñ. Ð¢Ð°ÐºÐ¶Ðµ Ð³Ð¸ÑÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ ÑÑÐµÐ·Ñ Ð¾ÑÐµÐ½Ð¸Ð²Ð°Ð»Ð¸ÑÑ Ð½Ð° Ð¿Ð°ÑÐ¾Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ. Ð ÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð¼ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÑÑÑÑ Ð´Ð°Ð½Ð½ÑÐµ Ð¿Ð¾ ÑÐ°ÑÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ ÑÐ°ÑÑÐ¸Ñ Ð² Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ðµ Ð¼ÑÑÐ¸ Ð½Ð° 2, 4, 7, 10 Ð¸ 15 Ð´ÐµÐ½Ñ Ð¿Ð¾ÑÐ»Ðµ Ð²Ð½ÑÑÑÐ¸Ð²ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð²Ð²ÐµÐ´ÐµÐ½Ð¸Ñ. ÐÑÐºÐ»Ð¾Ð½ÐµÐ½Ð¸Ð¹ Ð³Ð¸ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸ ÑÐºÐ°Ð½ÐµÐ¹ Ð¾Ñ Ð½Ð¾ÑÐ¼Ñ Ð½Ðµ Ð½Ð°Ð±Ð»ÑÐ´Ð°Ð»Ð¾ÑÑ ÐÐ°Ð½Ð½Ð¾Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÐµÑ Ð²ÑÐ²ÐµÑÑÐ¸ Ð´Ð°Ð½Ð½ÑÐ¹ ÑÐ¸Ð¿ Ð½Ð¾ÑÐ¸ÑÐµÐ»ÐµÐ¹ Ð½Ð° ÑÐ»ÐµÐ´ÑÑÑÐ¸Ð¹ ÑÑÐ°Ð¿ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ Ð¸ Ð¿ÑÐ¸Ð±Ð»Ð¸Ð·Ð¸ÑÑ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ Ð² ÐºÐ»Ð¸Ð½Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð¿ÑÐ°ÐºÑÐ¸ÐºÐµ. ÐÑÐ¾Ð¼Ðµ ÑÑÐ¾Ð³Ð¾, Ð¾Ð½Ð¾ Ð¿Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÐµÑ Ð¾ÑÐ½Ð¾Ð²Ð½ÑÐµ Ð¾ÑÐ³Ð°Ð½Ñ Ð½Ð°ÐºÐ¾Ð¿Ð»ÐµÐ½Ð¸Ñ ÑÐ°ÑÑÐ¸Ñ, ÑÑÐ¾ Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÐµÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°ÑÑ ÑÐ°ÑÑÐ¸ÑÑ Ð´Ð»Ñ Ð°Ð´ÑÐµÑÐ½Ð¾Ð¹ Ð´Ð¾ÑÑÐ°Ð²ÐºÐ¸.
description: The subject of the graduate qualification work is Â«Investigation of the distribution profile and dynamics of biodegradation of polyelectrolyte carriers of biologically active substances in vivoÂ». This work is devoted to the study the bio-distribution of micron particles obtained by Layer-by-Layer technology when layers of polyarginine and dextran sulfate were successively applied to the calcium carbonate core. The studies were carried out using various imaging methods, such as light and confocal microscopy (for histological sections), as well as fluorescence in vivo imaging. Histological sections were also evaluated for pathomorphological changes. The result of the work is data on the distribution of particles in the mouse on day 2, 4, 7, 10 and 15 after intravenous administration. No deviations of tissue histology from the norm were observed. This study allows bring this type of carrier to the next stage of research and makes it closer to clinical practice. In addition, it shows the main organs of particle accumulation, which allows you to use the particles for targeted delivery.