cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Supplementary figures and supplementary table captions from Co-phylogeny, narrow host breadth and local conditions drive highly specialised bird-haemosporidian associations in West-Central African sky islands
Creators: name: Bodawatta, Kasun H
name: Albrecht, Tomas
name: Krausová, Simona
name: Nana, Eric Djomo
name: Hořák, David
name: Sedláček, Ondřej
name: Jønsson, Knud Andreas
name: Munclinger, Pavel
Publisher: name: The Royal Society
Year: 2025
Subjects: classificationCode: 310399
subject: Ecology not elsewhere classified

description: This file contains the supplementary figures and supplementary table captions of the current publication.s
Effect of fumarate and live yeast on methane emissions, rumen fermentation, blood metabolites in dairy goats
Creators: name: 李宗军(ZongjunLi)
Publisher: name: CNGB
Year: 2024
Subjects: Raw sequence reads
Genome sequencing
Monoisolate

description: Fumarate inhibited hourly methane emission rate for 4 h after feeding. The TCA cycle and urea cycle were enhanced in fumarate-fed animals, likely due to part of the intaked fumarate was absorbed.
Microtrombicula (Microtrombicula) brachytrichia Brennan 1971
Creators: name: Nielsen, David H.
name: Robbins, Richard G.
name: Rueda, Leopoldo M.
Publisher: name: Zenodo
Year: 2021
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Arachnida
Actinedida
Trombiculidae
Microtrombicula
Microtrombicula brachytrichia

description: Microtrombicula (Microtrombicula) brachytrichia Brennan, 1971c:
NEO
Microtrombicula brachytrichia, Jacinavicius e t al. 2018c
description: Published as part of Nielsen, David H., Robbins, Richard G. & Rueda, Leopoldo M., 2021, Annotated world checklist of the Trombiculidae and Leeuwenhoekiidae (1758 - 2021) (Acari: Trombiculoidea), with notes on nomenclature, taxonomy, and distribution, pp. 1-243 in Zootaxa 4967 (1) on page 148, DOI: 10.11646/zootaxa.4967.1.1, http://zenodo.org/record/4745510
Fumarate supplementation improves the nutritional desirability of the milk fatty acid profiles in dairy goats
Creators: name: 李宗军(ZongjunLi)
Publisher: name: CNGB
Year: 2024
Subjects: Raw sequence reads
Genome sequencing
Monoisolate

description: Rumen microbial biohydrogenation (RBH) is the major factor responsible for the bovine milk rich in saturated fatty acids (FAs). Here we evaluated the effects of nutritional manipulation of ruminal propionogenesis and methanogenesis, two primary hydrogen sinks, on the RBH and milk FA profiles in vivo and in vitro. In the in vivo study, the combined effects of fumarate (FUM, a propionogenesis enhancer) and N-[2-(nitrooxy)ethyl]-3-pyridinecarboxamide (NPD, a methanogenesis inhibitor) were evaluated using 24 lactating dairy goats. In the in vitro study, the factors that can influence the anti-RBH potential of FUM were further investigated.
Additional file 16: Figure S6. of Network topology of NaV1.7 mutations in sodium channel-related painful disorders
Creators: name: Kapetis, Dimos
name: Sassone, Jenny
name: Yang, Yang
name: Galbardi, Barbara
name: Xenakis, Markos
name: Westra, Ronald
name: Szklarczyk, Radek
name: Lindsey, Patrick
name: Faber, Catharina
name: Gerrits, Monique
name: Merkies, Ingemar
name: Dib-Hajj, Sulayman
name: Mantegazza, Massimo
name: Waxman, Stephen
name: Lauria, Giuseppe
Publisher: name: Figshare
Year: 2017
Subjects: subject: Biophysics
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: 69999 Biological Sciences not elsewhere classified
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences

description: Structural modelling variants and their interatomic bonds of I848T and N395K. (DOCX 747Â kb)
Thaumatoniscellus Tabacaru 1973
Creators: name: Karaman, Ivo M.
name: Bedek, Jana
name: Horvatović, Mladen
Publisher: name: Zenodo
Year: 2009
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Malacostraca
Isopoda
Trichoniscidae
Thaumatoniscellus

description: Thaumatoniscellus Tabacaru, 1973
Type species, Thaumatoniscellus orghidani Tabacaru, 1973
Diagnosis emended. Small blind trichoniscid with stout body. Dorsum ornamented with rounded tubercles on head, pereon and pleonite 3. Head short with 2 rows of tubercles. Epimera of pleonites 3–5 well developed. Antennal flagellum three segmented. Maxilla 1 endopodite with 3 short and stout penicils. Maxilla 2 outer lobe narrow. Maxilliped endopodite with narrow latero-distal margin. Genital papilla anchor-like terminally. Male pleopod 1 exopodite voluminous, laterally constricted, terminally of complex three-dimensional structure; endopodite stout with reduced terminal article, shorter than exopodite.
description: Published as part of Karaman, Ivo M., Bedek, Jana & Horvatović, Mladen, 2009, Thaumatoniscellus speluncae n. sp. (Isopoda: Oniscidea: Trichoniscidae), a new troglobitic oniscid species from Croatia, pp. 57-64 in Zootaxa 2158 on page 57, DOI: 10.5281/zenodo.188890
wing primordium of the domestic silkworm
Creators: name: 何明敏(Mingmin)
Publisher: name: CNGB
Year: 2024
Subjects: Transcriptome or Gene expression
Monoisolate
Evolution

description: scRNA-seq data in the wing primordium of the domestic silkworm during normal development at 5th instar, 2nd day; 5th instar, 3rd day; 5th instar, 4th day; 5th instar, 5th day; 5th instar, 6th day; 5th instar, 7th day; WD period, 1st day; WD period, 2nd day; and P period.
snRNA-seq data in the wing primordium of the domestic silkworm after hormonal induction treatment following normal development until 5th instar, 2nd day; 0h; 10 min; 20 min; 30 min; 40 min; 50 min; 60 min; 4h; and 6h.
stereo-seq data of single cells in the wing primordium of the domestic silkworm during normal development at 5th instar, 2nd day; 5th instar, 3rd day; and WD period, 2nd day.
Additional file 7: Figure S3. of Network topology of NaV1.7 mutations in sodium channel-related painful disorders
Creators: name: Kapetis, Dimos
name: Sassone, Jenny
name: Yang, Yang
name: Galbardi, Barbara
name: Xenakis, Markos
name: Westra, Ronald
name: Szklarczyk, Radek
name: Lindsey, Patrick
name: Faber, Catharina
name: Gerrits, Monique
name: Merkies, Ingemar
name: Dib-Hajj, Sulayman
name: Mantegazza, Massimo
name: Waxman, Stephen
name: Lauria, Giuseppe
Publisher: name: Figshare
Year: 2017
Subjects: subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: 69999 Biological Sciences not elsewhere classified
subject: Science Policy

description: Clustering coefficient variation (â  CC ct ) in NaV1.7 mutations compared to WT. (DOCX 2798Â kb)
Nanopore-based Massively Parallel Peptide Characterization for Protein Identification
Creators: name: 王冀(Wangji)
Publisher: name: CNGB
Year: 2024
Subjects: Other
Peptide Sensing
Synthetic
Nanopore-based Protein Sequencing

description: Nanopore-based single-molecule sensing technology shows promising advantages in protein identification. Its real-world applications, however, are significantly constrained by the lack of streamlined sample preparation methods and low throughput, limited detection capabilities for variable samples. We herein present a nanopore sequencing platform that can accurately differentiate peptide fragments derived from enzymatically digested proteins by integrating a universal peptide library preparation strategy and AI-assisted algorithms, facilitating practical protein identification. Benefiting from massively parallel sensing data output, the given peptides exhibit unique statistical signal characteristics. This enables a reliable density-matrix (DM) based filtering approach that discriminates both positive and negative samples. Additionally, combining DMs from different peptides also reveals specific fingerprints among various proteins, enabling effective protein differentiation. The accurate discrimination of real samples has validated the feasibility and reliability of this workflow for nanopore array-based protein detection applications.
BCV improves tumor immune microenvironment and inhibits tumor growth by reshaping gut microbiota and bile acid metabolism
Creators: name: 韩艺(Zilong Zhu)
Publisher: name: CNGB
Year: 2024
Subjects: Metabolome
Multiisolate
Other
Antitumor immunity,Vaccine

description: In conclusion, our study suggests that BCV can up-regulate the abundance of L. reuteri to improve the intestinal microenvironment. L. reuteri enhances the effector functions of CD4+ and CD8+ T cells by down-regulating the level of TCA, and finally play an anti-tumor role. Therefore, BCV could be of great value in tumor therapy.
MOESM1 of
                     TCF21 is related to testis growth and development in broiler chickens
Creators: name: Zhang, Hui
name: Na, Wei
name: Zhang, Hong-Li
name: Wang, Ning
name: Du, Zhi-Qiang
name: Shou-Zhi Wang
name: Wang, Zhi-Peng
name: Zhiwu Zhang
name: Li, Hui
Publisher: name: Figshare
Year: 2017
Subjects: subject: Space Science
subject: Genetics
subject: FOS: Biological sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology

description: Additional file 1: Table S1. Generations of NEAUHLF chickens used for the different analyses in the current study.
Microtrombicula (Microtrombicula) brennani Goff, Whitaker and Dietz 1986
Creators: name: Nielsen, David H.
name: Robbins, Richard G.
name: Rueda, Leopoldo M.
Publisher: name: Zenodo
Year: 2021
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Arachnida
Actinedida
Trombiculidae
Microtrombicula
Microtrombicula brennani

description: Microtrombicula (Microtrombicula) brennani Goff, Whitaker and Dietz, 1986:
NEO
Microtrombicula brachytrichia, Jacinavicius et al. 2018c
description: Published as part of Nielsen, David H., Robbins, Richard G. & Rueda, Leopoldo M., 2021, Annotated world checklist of the Trombiculidae and Leeuwenhoekiidae (1758 - 2021) (Acari: Trombiculoidea), with notes on nomenclature, taxonomy, and distribution, pp. 1-243 in Zootaxa 4967 (1) on page 148, DOI: 10.11646/zootaxa.4967.1.1, http://zenodo.org/record/4745510
Multi-omics revealed immune determinants of severity in viral encephalitis
Creators: name: 周天炽(TIAN-CHI ZHOU)
Publisher: name: CNGB
Year: 2024
Subjects: Transcriptome or Gene expression
Multiisolate
Other
viral multi-omics

description: The underlying mechanisms of progression to severe viral encephalitis (VE) is not yet fully understood. In this study, we conducted multi-omics technology to differeniate the methanisms involved in mild and serere VE. Autoimmune encephalitis samples were included as controlled arms to further explore the similar and distinct immune pathogenic features.
Additional file 4: Figure S1. of Network topology of NaV1.7 mutations in sodium channel-related painful disorders
Creators: name: Kapetis, Dimos
name: Sassone, Jenny
name: Yang, Yang
name: Galbardi, Barbara
name: Xenakis, Markos
name: Westra, Ronald
name: Szklarczyk, Radek
name: Lindsey, Patrick
name: Faber, Catharina
name: Gerrits, Monique
name: Merkies, Ingemar
name: Dib-Hajj, Sulayman
name: Mantegazza, Massimo
name: Waxman, Stephen
name: Lauria, Giuseppe
Publisher: name: Figshare
Year: 2017
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: 69999 Biological Sciences not elsewhere classified
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences

description: Ramachandran plot of NaV1.7 WT. (DOCX 233Â kb)
MOESM8 of
                     TCF21 is related to testis growth and development in broiler chickens
Creators: name: Zhang, Hui
name: Na, Wei
name: Zhang, Hong-Li
name: Wang, Ning
name: Du, Zhi-Qiang
name: Shou-Zhi Wang
name: Wang, Zhi-Peng
name: Zhiwu Zhang
name: Li, Hui
Publisher: name: Figshare
Year: 2017
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: Developmental Biology

description: Additional file 8 Known functions of the remaining eight candidate genes. Description: This file describes the basic functions of the other eight genes detected in the GWAS and their possible involvement in spermatogenesis [39â  82].
Additional file 9: Figure S5. of Network topology of NaV1.7 mutations in sodium channel-related painful disorders
Creators: name: Kapetis, Dimos
name: Sassone, Jenny
name: Yang, Yang
name: Galbardi, Barbara
name: Xenakis, Markos
name: Westra, Ronald
name: Szklarczyk, Radek
name: Lindsey, Patrick
name: Faber, Catharina
name: Gerrits, Monique
name: Merkies, Ingemar
name: Dib-Hajj, Sulayman
name: Mantegazza, Massimo
name: Waxman, Stephen
name: Lauria, Giuseppe
Publisher: name: Figshare
Year: 2017
Subjects: subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: 69999 Biological Sciences not elsewhere classified
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences

description: Eccentricity centrality variation (â  E ct ) in NaV1.7 mutations compared to WT. (DOCX 2709Â kb)
A New Perspective on Physics Through Scale Dimension - A Thought Experiment
Creators: name: Ikida, Koji
Publisher: name: Zenodo
Year: 2024
Subjects: Scale Dimension, Thought Experiment, Theoretical Physics

description:  

This proposal presents a thought experiment in physics. While we usually regard time and space as the fundamental dimensions in our understanding of the universe, what if "scale" itself were introduced as a new fundamental dimension? How might this shift our perspective on physics?

Modern physics operates through distinct frameworks: quantum mechanics, classical physics, and relativity theory. Each of these theories successfully explains its respective domain, yet a unified understanding remains elusive.

This thought experiment explores the possibility of treating scale as a dimension that connects these frameworks. By introducing "scale" as a physical dimension, I propose the following:

Physical laws may be expressed as functions of scale.

Transitions between different scales (e.g., quantum to classical domains) could be mathematically described.

The shift from quantum mechanics to classical physics might be expressed as a continuous, unified process.

This is not presented as a formal theory but as an idea intended to encourage discussion and exploration among those interested in new perspectives on physics.
Discovery and mechanism of action of natural compounds against Salmonella infection based on pathogen-host interaction
Creators: name: 葛津利(Jinli Ge)
Publisher: name: CNGB
Year: 2024
Subjects: Genome sequencing and assembly
Multiisolate

description: Salmonella enterica, a food- and water-borne pathogen, triggers food poisoning and enteric infections. The effectiveness of antibiotics against Salmonella infections is decreasing due to bacterial resistance. Developing novel antimicrobial agents is crucial and urgent. Here, we screened 550 natural compounds and found that isopropyl paraben (IPPB) effectively inhibited Salmonella enterica serovar Typhimurium (ST) invasion of host cells without impacting bacterial growth, reducing the risk of developing bacterial resistance. Further investigation revealed that IPPB bound to the regulatory protein HilD and inhibited the transcription of the type III protein secretion system 1 (T3SS-1) regulatory genes hilD, hilC and rtsA. This interference blocked the production of T3SS-1 effectors. Importantly, IPPB exerted favorable therapeutic effects on both G. mellonella larvae and murine models infected with ST, and improved the ST-induced gut microbiota dysbiosis in mice. These findings suggest that IPPB has potential as a novel antimicrobial agent, targeting the T3SS-1 of Salmonella.
Actinopus insignis
Creators: name: Miglio, Laura Tavares
name: Pérez-Miles, Fernando
name: Bonaldo, Alexandre B.
Publisher: name: Zenodo
Year: 2020
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Arachnida
Araneae
Actinopodidae
Actinopus
Actinopus insignis

description: Actinopus insignis (Holmberg, 1881)
Aussereria insignis Holmberg, 1881: 171, pl. 1, fig. 2 (Holotype ♂, Provincia Bonaerensis, Chacabuco, Zárate, Buenos Aires, Argentina, ZMUC, not examined).
Actinopus insignis: Simon, 1892: 80; Ríos-Tamayo & Goloboff, 2018: 45, fig. 21 A–G; World Spider Catalog, 2020.
Diagnosis. Males of A. insignis resemble those of most species of the group pinhao by PA continuous to Pac and differ from those of A. pinhao (Fig. 32 A–C) by the lateral tegular swelling above PA in prolateral view, from A. paraitinga (Fig. 59 A–C) by serrated area at embolus base, from A. hirsutus (Fig. 38 A–C) and A. pinhao by the hirsute legs, and from A. hirsutus and A. cornelli (Fig. 62 A–C) by the PA continuous to PI. Females differ from those of A. pampulha (Fig. 49 D) and A. candango (Fig. 54 D) by the pore distribution reaching 75–100% of the receptacle length; and from A. paraitinga (Fig. 59 D) by the absence of lateral swellings on the receptacles, which are bilobed, with lateral lobes longer than inner lobes.
Description. See Ríos-Tamayo & Goloboff (2018: 45).
Distribution. ARGENTINA: BuenosAires,Córdoba, and Isla Martín Garcia. URUGUAY: Maldonado.
description: Published as part of Miglio, Laura Tavares, Pérez-Miles, Fernando & Bonaldo, Alexandre B., 2020, Taxonomic Revision of the Spider Genus Actinopus Perty, 1833 (Araneae, Mygalomorphae, Actinopodidae), pp. 1-256 in Megataxa 2 (1) on page 70, DOI: 10.11646/megataxa.2.1.1, http://zenodo.org/record/5597237
Whole Exome Sequencing Data for a 38-Week Pregnant Woman, G3P1 with a History of Cesarean Section and Low-Lying Placenta
Creators: name: 郭叶青(Guo Yeqing)
Publisher: name: CNGB
Year: 2024
Subjects: Raw sequence reads
Multispecies

description: This is a 38-year-old female, G3P1, at 38 weeks gestation with a history of cesarean section, presenting for delivery preparation. Her pregnancy course has been uneventful, with early symptoms resolving by 2 months and fetal movements detected at 4 months. She experienced episodes of vaginal bleeding at 4 and 6 months, which resolved spontaneously. No routine prenatal care was received, but NIPT was performed with a low-risk result. On examination, the patient is stable with normal vital signs and gravid abdomen. Obstetric examination reveals an estimated fetal weight of 3000 g, cephalic presentation with head engaged, and fetal heart rate of 140 bpm. Cervix is closed, posterior, soft, and 2 cm dilated. Pelvic inlet is adequate with a pelvis score of 7. Investigations include normal laboratory tests, negative serology for infectious diseases, and normal glucose tolerance test. Ultrasound reveals a singleton pregnancy with posterior placenta (Grade 1), corresponding to 35+1 weeks gestation, with an estimated fetal weight of 2632 g
Additional file 1: S1. of Network topology of NaV1.7 mutations in sodium channel-related painful disorders
Creators: name: Kapetis, Dimos
name: Sassone, Jenny
name: Yang, Yang
name: Galbardi, Barbara
name: Xenakis, Markos
name: Westra, Ronald
name: Szklarczyk, Radek
name: Lindsey, Patrick
name: Faber, Catharina
name: Gerrits, Monique
name: Merkies, Ingemar
name: Dib-Hajj, Sulayman
name: Mantegazza, Massimo
name: Waxman, Stephen
name: Lauria, Giuseppe
Publisher: name: Figshare
Year: 2017
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Physiology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology

description: Text. Phylogenenetic tree of human SCN9A and homologous genes. (DOCX 35Â kb)
Microtrombicula (Microtrombicula) brutsaerti , Vercammen-Grandjean 1965
Creators: name: Nielsen, David H.
name: Robbins, Richard G.
name: Rueda, Leopoldo M.
Publisher: name: Zenodo
Year: 2021
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Arachnida
Actinedida
Trombiculidae
Microtrombicula
Microtrombicula brutsaerti

description: Microtrombicula (Microtrombicula) brutsaerti Vercammen-Grandjean, 1965b:
AFR
Microtrombicula brutsaerti, Vercammen-Grandjean 1965d [minutissima group]; Stekolnikov 2018b
description: Published as part of Nielsen, David H., Robbins, Richard G. & Rueda, Leopoldo M., 2021, Annotated world checklist of the Trombiculidae and Leeuwenhoekiidae (1758 - 2021) (Acari: Trombiculoidea), with notes on nomenclature, taxonomy, and distribution, pp. 1-243 in Zootaxa 4967 (1) on page 148, DOI: 10.11646/zootaxa.4967.1.1, http://zenodo.org/record/4745510
Application of Metagenomic Next-Generation Sequencing
Creators: name: 杨巍(WeiYang)
Publisher: name: CNGB
Year: 2024
Subjects: Raw sequence reads
Multispecies

description: Background: Metagenomic high-throughput sequencing (mNGS) represents a powerful tool for detecting nucleic acids from various pathogens, such as bacteria, fungi, viruses and parasites, in clinical samples. Despite its extensively employed in the pathogen diagnosis for various infectious diseases, its application in diagnosing stroke-related infection, and its potential impact on clinical decision-making, anti-infection treatment, clinical intervention, and patient prognosis remain insufficiently explored. Additionally, while mNGS offers promising potential, it facts limitations related to sensitivity, specificity, cost, and standardization, which could influence its integration into routine clinical practice.
Methods: We retrospectively analyzed 18 stroke patients admitted to the First Affiliated Hospital of Medical College of Shantou University from January to February 2023, comparing culture-based methods with mNGS detection, and assessing its significance in etiological diagnosis. Additionally, we evaluated the performance differences among various sequencing platforms.
Results: Among the 18 stroke patients enrolled, pulmonary infections were identified in 7 cases, urinary tract infections in 1 case, central nervous system infections in 10 cases, and combined pulmonary and central nervous system infections in 2 cases, with 2 cases yielding negative results. mNGS detected pathogens in 13 cases, aligning with clinical diagnoses (75% concordance), whereas culture-based methods yielded positive results in only 6 cases (22% concordance). Importantly, for 9 of the 18 patients, adjustments to anti-infective treatment regimens based on mNGS results led to improved symptomatic relief and infection control. This suggests that mNGS can contribute to more timely and precise treatment modifications, particularly for infections with low pathogen loads, potentially enhancing clinical outcomes.
Conclusion: Our findings highlights the utility of mNGS in diagnosing stroke-associated infections by providing a more comprehensive etiological diagnosis compared to traditional method. While mNGS shows promise in enhancing diagnostic accuracy and guiding clinical treatment, it high cost and technical challenges need addressing before widespread clinical adoption. Future research should focus on optimizing mNGS protocols, integration it with convertional diagnostic tools, and evaluating its cost-effectiveness and clinical impact through larger, multicentric studies.
MOESM5 of
                     TCF21 is related to testis growth and development in broiler chickens
Creators: name: Zhang, Hui
name: Na, Wei
name: Zhang, Hong-Li
name: Wang, Ning
name: Du, Zhi-Qiang
name: Shou-Zhi Wang
name: Wang, Zhi-Peng
name: Zhiwu Zhang
name: Li, Hui
Publisher: name: Figshare
Year: 2017
Subjects: subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Physiology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Computational Biology

description: Additional file 5: Figure S2. Scatter plots of the expression level ( $$ 2^{{ -\Delta C_{\text{t}} }} $$ 2 - Î  C t ) of the TCF21 gene in testis tissue versus testis weight (TeW) in the lean and fat lines from G19 using two different housekeeping genes as internal references. TeWÂ =Â testis weight.
Additional file 1 of BaalChIP: Bayesian analysis of allele-specific transcription factor binding in cancer genomes
Creators: name: Santiago, Ines De
name: Liu, Wei
name: Yuan, Ke
name: OâReilly, Martin
name: Chilamakuri, Chandra Sekhar Reddy
name: Ponder, Bruce A. J.
name: Meyer, Kerstin B.
name: Markowetz, Florian
Publisher: name: Figshare
Year: 2017
Subjects: subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: Plant Biology

description: Supplementary Figures S1â  S16. (PDF 6500 kb)
Additional file 1 of BaalChIP: Bayesian analysis of allele-specific transcription factor binding in cancer genomes
Creators: name: Santiago, Ines De
name: Liu, Wei
name: Yuan, Ke
name: OâReilly, Martin
name: Chilamakuri, Chandra Sekhar Reddy
name: Ponder, Bruce A. J.
name: Meyer, Kerstin B.
name: Markowetz, Florian
Publisher: name: Figshare
Year: 2017
Subjects: subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Plant Biology

description: Supplementary Figures S1â  S16. (PDF 6500 kb)
Actinopus ramirezi Rios-Tamayo & Goloboff 2018
Creators: name: Miglio, Laura Tavares
name: Pérez-Miles, Fernando
name: Bonaldo, Alexandre B.
Publisher: name: Zenodo
Year: 2020
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Arachnida
Araneae
Actinopodidae
Actinopus
Actinopus ramirezi

description: Actinopus ramirezi Ríos-Tamayo & Goloboff, 2018
Actinopus ramirezi Ríos-Tamayo & Goloboff, 2018: 63, fig. 32 A–G, 33 A–E, 44 (holotype ♂, [25º 39’ S 54º 30’ W], entre El Timbó y El Palmital, R. P. 101, Parque Nacional Iguazú, Misiones, Argentina, 13–15.ix.1992, P. Goloboff & C. Szumik leg., MACN-Ar 198206; paratype ♀, same data as holotype, MACN-Ar 19830; not examined); World Spider Catalog, 2020.
Diagnosis. Males of A. ramirezi (Ríos-Tamayo & Goloboff, 2018, fig. 32 D–F) differ from those of most species of the pinhao group by PA continuous to PI. They resemble those of A. ducke (Fig. 35 A–C), A. hirsutus (Fig. 38 A–C), A. cornelli (Fig. 62 A–C) and A. insignis (Ríos-Tamayo & Goloboff, 2018, fig. 45 B–D) by PA continuous to PAc. They differ from those of A. ducke (Fig. 35 A–C) and A. confusus (Fig. 44 A–C) by BTA placed ventrally on prolateral tegular surface. They resemble those ofs A. pinhao (Fig. 32 A–C) and A. hirsutus (Fig. 37 A–D) by hirsute legs, differing from A. pinhao by the absence of tegular swelling above PA and from A. hirsutus by BTA most pronounced, PA less pronounced and a serrated area developed, extending to the base of the embolus and arranged in three rows. Females differ from those of A. pampulha (Fig. 49 D) and A. candango (Fig. 54 D) by pore distribution reaching only 75% of the receptacles length; and from A. paraitinga by the absence of lateral swelling on receptacles.
Description. See Ríos-Tamayo & Goloboff (2018: 63).
Distribution. ARGENTINA: Misiones.
description: Published as part of Miglio, Laura Tavares, Pérez-Miles, Fernando & Bonaldo, Alexandre B., 2020, Taxonomic Revision of the Spider Genus Actinopus Perty, 1833 (Araneae, Mygalomorphae, Actinopodidae), pp. 1-256 in Megataxa 2 (1) on pages 70-71, DOI: 10.11646/megataxa.2.1.1, http://zenodo.org/record/5597237
Additional file 2: Table S1. of Network topology of NaV1.7 mutations in sodium channel-related painful disorders
Creators: name: Kapetis, Dimos
name: Sassone, Jenny
name: Yang, Yang
name: Galbardi, Barbara
name: Xenakis, Markos
name: Westra, Ronald
name: Szklarczyk, Radek
name: Lindsey, Patrick
name: Faber, Catharina
name: Gerrits, Monique
name: Merkies, Ingemar
name: Dib-Hajj, Sulayman
name: Mantegazza, Massimo
name: Waxman, Stephen
name: Lauria, Giuseppe
Publisher: name: Figshare
Year: 2017
Subjects: subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics
subject: Cancer

description: NaV1.7 mutations associated to IEM, SFN and PEPD. (DOCX 50Â kb)
MOESM4 of
                     TCF21 is related to testis growth and development in broiler chickens
Creators: name: Zhang, Hui
name: Na, Wei
name: Zhang, Hong-Li
name: Wang, Ning
name: Du, Zhi-Qiang
name: Shou-Zhi Wang
name: Wang, Zhi-Peng
name: Zhiwu Zhang
name: Li, Hui
Publisher: name: Figshare
Year: 2017
Subjects: subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: Developmental Biology

description: Additional file 4: Figure S1. Manhattan and Q–Q plots of genome-wide association analyses for TeW and TeP, generated with GAPIT, EMMAX, GenABEL (GRAMMAR) and GEMMA software. The solid line indicates genome-wide significance of association (P value <5.48 × 10−7). TeW testis weight, TeP testis percentage.
Septobasidium macrobasidium Q. Q. Jiang & C. L. Zhao 2024, sp. nov.
Creators: name: Jiang, Qianquan
name: Kang, Zhengli
name: Wang, Xubo
name: Zhao, Changlin
Publisher: name: Zenodo
Year: 2024
Subjects: Biodiversity
Taxonomy
Fungi
Basidiomycota
Pucciniomycetes
Septobasidiales
Septobasidiaceae
Septobasidium
Septobasidium macrobasidium

description: Septobasidium macrobasidium Q. Q. Jiang & C. L. Zhao sp. nov.
Figs 3, 4
Holotype.
China • Yunnan Province, Pu’er, Jingdong County, Wuliangshan National Nature Reserve, 24°29'17"N, 100°40'27"E, altitude: 1800 m a. s. l., on the living tree of angiosperm, leg. C. L. Zhao, 6 January 2019, CLZhao 9624 (SWFC).
Diagnosis.
Differs from other Septobasidium species by the coriaceous basidiomata with a cream surface, cylindrical basidia (48.5–83 × 6.5–13 µm), and thin-walled, narrowly cylindrical basidiospores with septa, measuring as 7–9 × 3.5–4.5 µm.
Etymology.
Macrobasidium (Lat.): refers to the larger basidia of the type specimen.
Description.
Basidiomata perennial, resupinate, easy to separate from substrate, coriaceous upon drying, up to 9 cm long, 5 cm wide, 2 mm thick. Hymenial surface smooth, slightly cream when fresh, cream upon drying. Sterile margin narrow, white to cream, up to 0.5 mm.
Hyphal system monomitic, generative hyphae with simple septa, pale brown, thick-walled, frequently branched, interwoven. In section 850–1850 µm thick; subiculum pale brown, 30–80 µm thick; pillars brown, 350–550 µm high, 85–200 µm wide, hyphae of pillars 2–5 µm thick, hyaline or brown, forming 2–3 horizontal layers.
Basidia arising directly from the generative hyphae, basidia cylindrical, straight, 4 - celled, 48.5–83 × 6.5–13 µm, colourless. Probasidia subglobose or ovoid, 13–23 × 7–20.5 µm, colourless, persistent. Haustoria consisting of irregularly coiled hyphae.
Basidiospores narrowly cylindrical, colourless, thin-walled, with septa, (6.5 –) 7–9 (– 9.5) × (3 –) 3.5–4.5 (– 5) µm, L = 8.09 µm, W = 3.94 µm, Q = 1.55–2.83 (n = 60 / 2).
Habitat and distribution.
Growing on the plant Betulaceae Gray, associated with the insect of Diaspididae Ferris.
Additional specimen examined
(paratype). China • Yunnan Province, Pu’er, Jingdong County, Wuliangshan National Nature Reserve, 24°29'17"N, 100°40'27"E, altitude: 1800 m a. s. l., on the living tree of angiosperm, leg. C. L. Zhao, 6 January 2019, CLZhao 9658 (SWFC).
description: Published as part of Jiang, Qianquan, Kang, Zhengli, Wang, Xubo & Zhao, Changlin, 2024, Molecular phylogeny and morphology reveal three new plant pathogenic fungi species (Septobasidiales, Basidiomycota) from China, pp. 229-248 in MycoKeys 111 on pages 229-248, DOI: 10.3897/mycokeys.111.125933
Actinopus Perty 1833
Creators: name: Miglio, Laura Tavares
name: Pérez-Miles, Fernando
name: Bonaldo, Alexandre B.
Publisher: name: Zenodo
Year: 2020
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Arachnida
Araneae
Actinopodidae
Actinopus

description: Group nattereri
Diagnosis. The species in the group nattereri are recognized by the following combination of characters: Medium to large in total length; embolus with three keels; BTA developed; serrated area above PAc in prolateral view reduced. Females are recognized by the spermathecae with two receptacles, without lateral swelling.
Species included. Actinopus nattereri (Doleschall, 1871); Actinopus rufipes (Lucas, 1834); Actinopus vilhena sp. nov.; Actinopus harveyi sp. nov.; Actinopus itapitocai sp. nov.; Actinopus ipioca sp. nov.; Actinopus itaqui sp. nov.; Actinopus xingu sp. nov.; Actinopus mesa sp. nov.; Actinopus caxiuana sp. nov.; Actinopus utinga sp. nov.; Actinopus reycali Ríos-Tamayo & Goloboff, 2018; and Actinopus septemtrionalis Ríos-Tamayo & Goloboff, 2018.
description: Published as part of Miglio, Laura Tavares, Pérez-Miles, Fernando & Bonaldo, Alexandre B., 2020, Taxonomic Revision of the Spider Genus Actinopus Perty, 1833 (Araneae, Mygalomorphae, Actinopodidae), pp. 1-256 in Megataxa 2 (1) on page 71, DOI: 10.11646/megataxa.2.1.1, http://zenodo.org/record/5597237
Septobasidium puerense Q. Q. Jiang & C. L. Zhao 2024, sp. nov.
Creators: name: Jiang, Qianquan
name: Kang, Zhengli
name: Wang, Xubo
name: Zhao, Changlin
Publisher: name: Zenodo
Year: 2024
Subjects: Biodiversity
Taxonomy
Fungi
Basidiomycota
Pucciniomycetes
Septobasidiales
Septobasidiaceae
Septobasidium
Septobasidium puerense

description: Septobasidium puerense Q. Q. Jiang & C. L. Zhao sp. nov.
Figs 5, 6
Holotype.
China • Yunnan Province, Pu’er, Jingdong County, The Forest of Pineapple, 24°30'58"N, 100°52'31"E, altitude: 2000 m a. s. l., on the living tree of angiosperm, leg. C. L. Zhao, 4 January 2019, CLZhao 9430 (SWFC).
Diagnosis.
Differs from other Septobasidium species by a cinnamon brown to chestnut brown surface, subglobose to pyriform probasidia (10.5–19.5 × 5.5–9 µm), and two types of haustoria consisting of irregularly coiled hyphae and spindle-shape.
Etymology.
Puerense (Lat.): refers to the locality (Pu’er) of the type specimen.
Description.
Basidiomata perennial, resupinate, hard to separate from substrate, coriaceous upon drying, up to 15 cm long, 1 cm wide, 1 mm thick. Hymenial surface smooth, pale brown when fresh, cinnamon brown to chestnut brown upon drying. Sterile margin slightly brown, up to 1 mm.
Hyphal system monomitic, generative hyphae with simple septa, pale brown, thick-walled. In section 380–650 µm thick; subiculum pale brown, 10–30 µm thick; pillars brown, 170–380 µm high, 40–85 µm wide, hyphae of pillars 1.5–3.5 µm thick, colorless, with closely packed parallel upright threads, forming 2–3 horizontal layers.
Basidia arising directly from the generative hyphae, cylindrical or slightly irregular, slightly curved, 2-3 - celled, 17–30.5 × 3–6.5 µm, colourless. Probasidia subglobose to pyriform, 10.5–19.5 × 5.5–9 µm, colorless, probasidia cell persistent after the formation of the basidia. Basidiospores not seen. Haustoria with two types: 1) consisting of irregularly coiled hyphae; 2) spindle-shape.
Habitat and distribution.
Growing on the plant Berberidaceae Juss, associated with the insect species Pseudaulacaspis pentagona (Targioni Tozzetti).
Additional specimen examined
(paratype). China • Yunnan Province, Pu’er, Jingdong Country, Wuliangshan National Nature Reserve, 24°29'17"N, 100°40'27"E, altitude: 1800 m a. s. l., on the living tree of angiosperm, leg. C. L. Zhao, 5 October 2017, CLZhao 4298 (SWFC).
description: Published as part of Jiang, Qianquan, Kang, Zhengli, Wang, Xubo & Zhao, Changlin, 2024, Molecular phylogeny and morphology reveal three new plant pathogenic fungi species (Septobasidiales, Basidiomycota) from China, pp. 229-248 in MycoKeys 111 on pages 229-248, DOI: 10.3897/mycokeys.111.125933
Microtrombicula (Microtrombicula) bruynoghei
Creators: name: Nielsen, David H.
name: Robbins, Richard G.
name: Rueda, Leopoldo M.
Publisher: name: Zenodo
Year: 2021
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Arachnida
Actinedida
Trombiculidae
Microtrombicula
Microtrombicula bruynoghei

description: Microtrombicula (Microtrombicula) bruynoghei (Jadin and Vercammen-Grandjean, 1952):
AFR
Trombicula bruynoghei Jadin and Vercammen-Grandjean, 1952; Audy & Vercammen-Grandjean, 1961a
Eltonella (Eltonella) bruynoghei, Vercammen-Grandjean 1965b
Microtrombicula bruynoghei, Stekolnikov 2018b
description: Published as part of Nielsen, David H., Robbins, Richard G. & Rueda, Leopoldo M., 2021, Annotated world checklist of the Trombiculidae and Leeuwenhoekiidae (1758 - 2021) (Acari: Trombiculoidea), with notes on nomenclature, taxonomy, and distribution, pp. 1-243 in Zootaxa 4967 (1) on page 148, DOI: 10.11646/zootaxa.4967.1.1, http://zenodo.org/record/4745510
Heptacanthella Gao & Potapov & Bu 2022, gen. nov.
Creators: name: Gao, Yan
name: Potapov, Mikhail
name: Bu, Yun
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Collembola
Entomobryomorpha
Isotomidae
Heptacanthella

description: Heptacanthella gen. nov.
Type species. Heptacanthella yinae gen. et sp. nov. here designated.
Diagnosis. Blind Anurophorinae without furca and with four and three anal spines on Abd.V and VI, respectively. Spines on Abd.VI larger than on Abd.V. Body slender. Pigmentation and eyes absent. With few setae on body. Integument finely reticulated, primary granules fused forming polygons of different shape. Unpaired median seta p0 absent on Abd. IV. Furca and tenaculum absent. Macrosetae and s-seta on body well differentiated.
Etymology. The genus name refers the Latin “ hepta ” indicating seven anal spines at the end of abdomen.
Species included. Only the type species, Heptacanthella yinae sp. nov.
Remarks. The new genus has an unpaired anal spine in m0 position in Abd.VI. This uncommon character is shared only with Tuvia, Octodonthophora and Pentacanthella (Table 1). Considering homology of all anal spines, the new genus most closely resembles and is probably related to the Asiatic genus Tuvia. The two genera have three anal spines in m0 and m1 position on Abd. VI, and two in p1 position on Abd. V. Heptacanthella gen. nov. has further p2 setae modified to spines. Accordingly, the new genus has four (vs. two in Tuvia) anal spines on Abd. V. Tuvia has eyes, pigmentation and furca (vs. absent in the new genus).
* 1 medial conical protuberance in Sahacanthella
Considering only characters associated with dwelling in deep soil layers (no pigmenatatioan and eyes, no furca) the new genus is formally similar to Yosiiella, Martynovella, and Dimorphacanthella. The four genera share absence of eyes, pigmentation, furca and tenaculum. Heptacanthella gen. nov. resembles more closely Yosiiella (South America) by the presence of anal spines on Abd.VI. The two genera differ in number of spines (as a whole, four and three in the new genus vs. two and two in Yosiiella, on Abd. V and VI, respectively) and cuticle (clearly reticulated vs. smooth). Martynovella and Dimorphacanthella (Asia) have no anal spines on Abd. VI (present in Heptacanthella gen. nov. and Yosiiella).
description: Published as part of Gao, Yan, Potapov, Mikhail & Bu, Yun, 2022, A new blind genus of Anurophorinae (Collembola: Isotomidae) with seven anal spines from Northwest China, pp. 80-88 in Zootaxa 5104 (1) on page 81, DOI: 10.11646/zootaxa.5104.1.4, http://zenodo.org/record/6258051
Protocol_micro-credentialing_.pdf
Creators: name: Oliver, Kim
Publisher: name: figshare
Year: 2024
Subjects: classificationCode: 420311
subject: Health systems
classificationCode: 420309
subject: Health management

description: This scoping review aims to understanding what the demand and structure of micro-credentials in healthcare. The delivery of education to health professionals is a ever-changing environment which requires tailored and targeted educational design to meet the needs of the workforce today.
Septobasidium wuliangshanense Q. Q. Jiang & C. L. Zhao 2024, sp. nov.
Creators: name: Jiang, Qianquan
name: Kang, Zhengli
name: Wang, Xubo
name: Zhao, Changlin
Publisher: name: Zenodo
Year: 2024
Subjects: Biodiversity
Taxonomy
Fungi
Basidiomycota
Pucciniomycetes
Septobasidiales
Septobasidiaceae
Septobasidium
Septobasidium wuliangshanense

description: Septobasidium wuliangshanense Q. Q. Jiang & C. L. Zhao sp. nov.
Figs 7, 8
Holotype.
China • Yunnan Province, Pu’er, Zhenyuan County, Heping Town, Liangzi Village, Wuliangshan National Nature Reserve, 24°29'17"N, 100°40'27"E, altitude: 1860 m a. s. l., on the living tree of angiosperm, leg. C. L. Zhao, 15 January 2018, CLZhao 5809 (SWFC).
Diagnosis.
Differs from other Septobasidium species by the coriaceous basidiomata with a slightly brown surface, cylindrical or slightly irregular basidia, pyriform to subglobose or ovoid probasidia (7.5–13 × 4.5–9 µm), and the haustoria consisting of irregularly coiled hyphae.
Etymology.
Wuliangshanense (Lat.): refers to the locality (Wuliangshan) of the type specimen.
Description.
Basidiomata perennial, resupinate, easy to separate from substrate, coriaceous upon drying, up to 10 cm long, 2 cm wide, 1 mm thick. Hymenial surface smooth, cream to pale brown when fresh, slightly brown upon drying. Sterile margin cream to slightly brown, up to 2 mm.
Hyphal system monomitic, generative hyphae with simple septa, pale brown, thick-walled. In section 660–1200 µm thick; subiculum pale brown, 20–50 µm thick; pillars brown, 150–300 µm high, 30–150 µm wide, hyphae of pillars 3–4 µm thick, brown, forming 2–3 horizontal layers.
Basidia arising directly from the generative hyphae, cylindrical or slightly irregular, colourless, straight or slightly curved, 2-3 - celled, 21.5–29 × 5.5–9 µm. Probasidia pyriform, subglobose or ovoid, 7.5–13 × 4.5–9 µm, colorless, probasidia cell persistent after the formation of the basidia. Haustoria consisting of irregularly coiled hyphae. Basidiospores not seen.
Habitat and distribution.
Growing on the plant Fagaceae Dumort, associated with the insect genus Aulacaspis Cockerell.
Additional specimen examined
(paratype). China • Yunnan Province, Pu’er, Jingdong County, Wuliangshan National Nature Reserve, in association with the genus Aulacaspis on Rosaceae, 24°29'17"N, 100°40'27"E, altitude: 1800 m a. s. l., on the living tree of angiosperm, leg. C. L. Zhao, 2 October 2017, CLZhao 3666 (SWFC).
description: Published as part of Jiang, Qianquan, Kang, Zhengli, Wang, Xubo & Zhao, Changlin, 2024, Molecular phylogeny and morphology reveal three new plant pathogenic fungi species (Septobasidiales, Basidiomycota) from China, pp. 229-248 in MycoKeys 111 on pages 229-248, DOI: 10.3897/mycokeys.111.125933
Additional file 1: Figure S1. of Economic co-production of poly(malic acid) and pullulan from Jerusalem artichoke tuber by Aureobasidium pullulans HA-4D
Creators: name: Xia, Jun
name: Jiaxing Xu
name: Xiaoyan Liu
name: Jiming Xu
name: Xingfeng Wang
name: Xiangqian Li
Publisher: name: Figshare
Year: 2017
Subjects: subject: Biophysics
subject: Biochemistry
subject: 29999 Physical Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: FOS: Biological sciences
subject: Developmental Biology
subject: Plant Biology

description: Structural characterization of the exopolysaccharide with FT-IR spectrum (a), 1H-NMR spectrum (b) and 13C-NMR spectrum (c). (DOCX 275Â kb)
Additional file 1: Table S1. of Transposable Element Bm1645 is a Source of BmAGO2-associated Small RNAs that affect its expression in Bombyx mori
Creators: name: Hongqiang Si
name: Yunjie Cao
name: Honglin Zhu
name: Li, Dan
name: Zhengbing Lv
name: Sheng, Qing
name: Zuoming Nie
Publisher: name: Figshare
Year: 2017
Subjects: subject: Biochemistry
subject: Space Science
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Molecular Biology
subject: Cancer
subject: 60506 Virology

description: The BmAGO2-associated TEs with abundance more than 100 and their mapped BmAGO2-associated small RNAs*. Table S2. The wild-type sequences of Bm1645 containing TE-siRNA targeted site and mutant sequences for dual-luciferase reporter vector construction. Figure S1. (A) Western blotting analysis of HIS-BmAGO2 isolated by immunoprecipitation. BmAGO2 was expressed in BmN cells infected with recombinant BmNPV virus. IP was validated by western blotting. (B) Co-immunoprecipitation RNAs analyzed by electrophoresis. M: marker. 1: RNAs pulled down by HIS monoclonal antibody. 2: RNAs pulled down by mouse IgG. The lane 2 was the negative control. Asterisks indicate the visible bands for the ~20 nt, ~27 nt and ~33 nt small RNA species, respectively. The box in lane 1 indicate the >200 nt fraction extracted for deep sequencing. (DOC 137 kb)
Additional file 1: Table S1. of Transposable Element Bm1645 is a Source of BmAGO2-associated Small RNAs that affect its expression in Bombyx mori
Creators: name: Hongqiang Si
name: Yunjie Cao
name: Honglin Zhu
name: Li, Dan
name: Zhengbing Lv
name: Sheng, Qing
name: Zuoming Nie
Publisher: name: Figshare
Year: 2017
Subjects: subject: Biochemistry
subject: Space Science
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Molecular Biology
subject: Cancer
subject: Virology

description: The BmAGO2-associated TEs with abundance more than 100 and their mapped BmAGO2-associated small RNAs*. Table S2. The wild-type sequences of Bm1645 containing TE-siRNA targeted site and mutant sequences for dual-luciferase reporter vector construction. Figure S1. (A) Western blotting analysis of HIS-BmAGO2 isolated by immunoprecipitation. BmAGO2 was expressed in BmN cells infected with recombinant BmNPV virus. IP was validated by western blotting. (B) Co-immunoprecipitation RNAs analyzed by electrophoresis. M: marker. 1: RNAs pulled down by HIS monoclonal antibody. 2: RNAs pulled down by mouse IgG. The lane 2 was the negative control. Asterisks indicate the visible bands for the ~20 nt, ~27 nt and ~33 nt small RNA species, respectively. The box in lane 1 indicate the >200 nt fraction extracted for deep sequencing. (DOC 137 kb)
Sterile Manufacturing of Drug Products and Their Applications to Bacteriophages
Creators: name: Gin, Aaron J
Publisher: name: Purdue University Graduate School
Year: 2024
Subjects: classificationCode: 310113
subject: Synthetic biology
classificationCode: 340401
subject: Biologically active molecules
classificationCode: 401801
subject: Micro- and nanosystems

description: Sterile manufacturing is multifaceted. Each aspect seeks to improve the process of producing drug products absent of impurities. Bacteriophages can benefit greatly from sterile manufacturing which would amplify their already vast range of applications. Novel bacteriophage discovery and annotation, implemented within a classroom setting, can aid in building the foundation of bacteriophages for use in clinical applications. High-Performance Liquid Chromatography (HPLC) can detect impurities and test compatibilities in the final product. Oxytocin and its interaction with tranexamic acid (TXA) provide an excellent example of how HPLC use can be critical in sterile manufacturing as well as build a baseline for which bacteriophages may be utilized in sterile production. A quality scorecard for drug products provides an additional metric that can be used by governing agencies and consumers to analyze drug products of similar bioequivalence and subsequently grade them. The development of a scorecard will provide a guideline to improve the sterile manufacturing of drug products and biologics such as bacteriophages. A literary analysis of lipid nanoparticles presents a future application for synthetically manufactured bacteriophages. The conclusions gathered from this work can be utilized as a case study for working professionals who aim to implement advancements in sterile manufacturing within their industry.