Metadata record for the manuscript: Stabilization of p18 by deubiquitylase CYLD is pivotal for cell cycle progression and viral replication
Creators: name: Li, Yueshuo
name: Shi, Feng
name: Hu, Jianmin
name: Xie, Longlong
name: Zhao, Lin
name: Tang, Min
name: Luo, Xiangjian
name: Ye, Mao
name: Zheng, Hui
name: Zhou, Min
name: Liu, Na
name: Bode, Ann M.
name: Fan, Jia
name: Zhou, Jian
name: Gao, Qiang
name: Qiu, Shuangjian
name: Wu, Weizhong
name: Zhang, Xin
name: Liao, Weihua
name: Cao, Ya
Publisher: name: figshare
Year: 2021
Subjects: Medical and Health Sciences not elsewhere classified
Oncology and Carcinogenesis not elsewhere classified
Cancer
Biological Sciences not elsewhere classified
Molecular Biology
Pathogenesis

description: <b>Summary</b>

This
metadata record provides details of the data supporting the claims of the
related manuscript: “Stabilization of p18 by
deubiquitylase CYLD is pivotal for cell cycle progression and viral replication
”.

The
related study aimed to identify a post translational regulator of protein
abundance of p18, a key negative regulator of cell cycle progression which
mediates cell cycle arrest at the G1/S phase.

 

Type of data:
histology images, patient survival data, head and neck squamous cell cancer
(HNSCC) patient survival data, nasopharyngeal carcinoma (NPC) patients’
cylindromatosis (CYLD) protein expression data, GSEA (Gene Set Enrichment
Analysis) data. 

Subject of
data: human NPC cell lines; immortalized human nasopharyngeal epithelial cell
lines; Nasopharyngitis and NPC tissues; BCLB/c-nu, female, 5 weeks old.

Sample size:
6

 

<b>Data
access</b>

The histology
images and patient survival data are not publicly available for the following
reason: data contain information that could
compromise research participant privacy. However, the data can be made
available upon reasonable request to the corresponding author. 

 

The NPC
patients CYLD expression data used in the study can be accessed via Oncomine (https://www.oncomine.org/) via the
following process. First, sign in with a registered mailbox. Then search with
Gene: CYLD; Analysis Type: Cancer vs. Normal Analysis; Cancer Type: Head and
Neck Cancer. Log-in is required to access Oncomine data. The data are freely
available for academic and non-profit communities.

 

The HNSCC patient survival data used
in the study are stored in the Excel file ‘HNSCC patient survival data.xlsx’
and are publicly available via The Cancer Genome Atlas (TCGA). SurvExpress (http://bioinformatica.mty.itesm.mx:8080/Biomatec/SurvivaX.jsp)
was used to analyse the data on TCGA, and Internal ID: 100.

 

The GSEA data used in the study are
openly available via the Gene Expression Omnibus repository via accession https://identifiers.org/geo:GSE12452. 

 

The reagents
and other materials (except for the received cells and plasmids) are available
from the corresponding author upon request.

 

<b>Corresponding author(s) for this study</b>

Ya
Cao, Key Laboratory of Carcinogenesis
and Cancer Invasion, Chinese Ministry of Education, Department of Radiology,
Xiangya Hospital, Central South University, Xiangya Road 110, Changsha, Hunan,
China; 410078; Tel: 86-731-84805448 (office); Email: ycao98@vip.sina.com.

<br>
<b>Study approval </b>

The
study was approved by the Medical Ethics Committee of Xiangya Hospital, Central
South University (No. 201803134).