title: Ð¡Ð±Ð¾ÑÐºÐ° Ð²Ð½ÑÑÑÐµÐ½Ð½ÐµÐ³Ð¾ ÑÐµÐ»Ð° Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð° phiKZ Ð² Ñ Ð¾Ð´Ðµ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Assembly of the inner body of bacteriophage phiKZ during infection
Creators: name: ÐÐ¸ÑÐ¸Ð¿Ð¾ÑÐµÐ½ÐºÐ¾, ÐÐ½Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: pseudomonas aeruginosa
Ð³Ð¸Ð³Ð°Ð½ÑÑÐºÐ¸Ð¹ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³ phiKZ
ÑÐ¿Ð°ÐºÐ¾Ð²ÐºÐ° ÐÐÐ
Ð²Ð½ÑÑÑÐµÐ½Ð½ÐµÐµ ÑÐµÐ»Ð¾
Ð¿ÑÐµÐ²Ð´Ð¾-ÑÐ´ÑÐ¾
ÑÐ±Ð¾ÑÐºÐ° Ð²Ð¸ÑÑÑÐ½ÑÑ ÑÐ°ÑÑÐ¸Ñ
ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð°Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ
jumbo bacteriophage phiKZ
DNA packaging
inner body
pseudo-nucleus
phage assembly
fluorescence microscopy

description: Ð Ð°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð² ÐºÐ»ÐµÑÐºÐµ Ð±ÐµÐ»ÐºÐ¾Ð² Ð²Ð½ÑÑÑÐµÐ½Ð½ÐµÐ³Ð¾ ÑÐµÐ»Ð° Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð° phiKZ Ð² ÑÐ¾Ð´Ðµ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸ Ð¸ Ð²ÐºÐ»ÑÑÐµÐ½Ð¸Ñ Ð¸Ñ Ð² ÑÐ¾Ð·ÑÐµÐ²Ð°ÑÑÑÑ Ð³Ð¾Ð»Ð¾Ð²ÐºÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð°. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ ÑÐ°Ð±Ð¾ÑÐµ Ð±ÑÐ»Ð¸ ÑÐ¾Ð·Ð´Ð°Ð½Ñ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ðµ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ð¸, Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÑÑÐ¸Ðµ ÑÐºÑÐ¿ÑÐµÑÑÐ¸ÑÐ¾Ð²Ð°ÑÑ Ð² Pseudomonas aeruginosa ÑÐ»Ð¸ÑÑÐµ Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð¹ Ð¼ÐµÑÐºÐ¾Ð¹ Ð·ÑÐµÐ»ÑÐµ ÑÑÐ°Ð³Ð¼ÐµÐ½ÑÑ Ð±ÐµÐ»ÐºÐ¾Ð² Ð²Ð½ÑÑÑÐµÐ½Ð½ÐµÐ³Ð¾ ÑÐµÐ»Ð°. ÐÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð°Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ Ð¸Ð½ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ phiKZ ÐºÐ»ÐµÑÐ¾Ðº, ÑÐºÑÐ¿ÑÐµÑÑÐ¸ÑÑÑÑÐ¸Ñ Ð¾Ð´Ð¸Ð½ Ð¸Ð· Ð³ÐµÐ½Ð¾Ð² Ð²Ð½ÑÑÑÐµÐ½Ð½ÐµÐ³Ð¾ ÑÐµÐ»Ð°. ÐÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð¼ÐµÑÐµÐ½ÑÐµ Ð±ÐµÐ»ÐºÐ¸ Ð²Ð½ÑÑÑÐµÐ½Ð½ÐµÐ³Ð¾ ÑÐµÐ»Ð° Ð²ÐºÐ»ÑÑÐ°ÑÑÑÑ Ð² ÑÐ¾ÑÑÐ°Ð² Ð´Ð¾ÑÐµÑÐ½Ð¸Ñ Ð²Ð¸ÑÐ¸Ð¾Ð½Ð¾Ð². ÐÐµÐ»ÐºÐ¸ mNG-Gp97 Ð¸ mNG-Gp162 ÑÐ¿Ð°ÐºÐ¾Ð²ÑÐ²Ð°ÑÑÑÑ Ð² ÐºÐ°Ð¿ÑÐ¸Ð´Ñ Ð¿ÐµÑÐ²ÑÐ¼Ð¸, Ð²Ð¾ Ð²ÑÐµÐ¼Ñ Ð´Ð²Ð¸Ð¶ÐµÐ½Ð¸Ñ ÑÐ°Ð³Ð¾Ð²ÑÑ Ð³Ð¾Ð»Ð¾Ð²Ð¾Ðº Ð¿Ð¾ Ð²ÐµÑÐµÑÐµÐ½Ñ Ðº Ð¿ÑÐµÐ²Ð´Ð¾-ÑÐ´ÑÑ. Gp90-mCh, Gp93-mCh, mNG-Gp95 Ð²ÐºÐ»ÑÑÐ°ÑÑÑÑ Ð² Ð²Ð¸ÑÐ¸Ð¾Ð½ Ð½Ð° Ð¿Ð¾Ð²ÐµÑÑÐ½Ð¾ÑÑÐ¸ Ð¿ÑÐµÐ²Ð´Ð¾-ÑÐ´ÑÐ°. ÐÐ¸ Ð¾Ð´Ð¸Ð½ Ð¸Ð· Ð¿ÑÑÐ¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð½ÑÑ Ð±ÐµÐ»ÐºÐ¾Ð² Ð½Ðµ Ð·Ð°ÑÐ¾Ð´Ð¸Ñ Ð² Ð¿ÑÐµÐ²Ð´Ð¾-ÑÐ´ÑÐ¾. ÐÐµÑÑ Ð¿ÑÐ¾ÑÐµÑÑ ÑÐ±Ð¾ÑÐºÐ¸ Ð²Ð½ÑÑÑÐµÐ½Ð½ÐµÐ³Ð¾ ÑÐµÐ»Ð° Ð¿ÑÐ¾Ð¸ÑÑÐ¾Ð´Ð¸Ñ Ð² ÑÐ¸ÑÐ¾Ð¿Ð»Ð°Ð·Ð¼Ðµ. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð´Ð°ÑÑ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÐµÐ½Ð¸Ðµ Ð¾ ÑÐ±Ð¾ÑÐºÐ¸ Ð²Ð½ÑÑÑÐµÐ½Ð½ÐµÐ³Ð¾ ÑÐµÐ»Ð° Ð³Ð¸Ð³Ð°Ð½ÑÑÐºÐ¸Ñ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð¾Ð² Ð² ÑÐ¾Ð´Ðµ ÑÐ¾Ð·ÑÐµÐ²Ð°Ð½Ð¸Ñ Ð²Ð¸ÑÑÑÐ½ÑÑ ÑÐ°ÑÑÐ¸Ñ. ÐÐ°Ð½Ð½ÑÐµ Ð¾ Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð±ÐµÐ»ÐºÐ¾Ð² Ð²Ð½ÑÑÑÐµÐ½Ð½ÐµÐ³Ð¾ ÑÐµÐ»Ð° phiKZ Ð´Ð¾Ð¿Ð¾Ð»Ð½ÑÑ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÐµÐ½Ð¸Ðµ Ð¾ ÑÑÐ½ÐºÑÐ¸ÑÑ ÑÐ°Ð³Ð¾Ð²ÑÑ Ð±ÐµÐ»ÐºÐ¾Ð² Ñ Ð½Ð¸Ð·ÐºÐ¾Ð¹ Ð³Ð¾Ð¼Ð¾Ð»Ð¾Ð³Ð¸ÐµÐ¹.
description: The thesis is devoted to the study of cell localization of proteins of the inner body of bacteriophage phiKZ during infection and their inclusion in the maturing head of bacteriophage. As a result of the work, plasmid vectors were created to express in Pseudomonas aeruginosa fused with fluorescent mark mature fragments of proteins of the inner body. Fluorescent microscopy of infected phiKZ cells expressing one of the genes of the inner body was performed. It has been shown that the tagged proteins of the inner body are incorporated into the new virions. The mNG-Gp97 and mNG-Gp162 proteins pack into capsids first, during spindle movement of phage heads to the phage nucleus. Gp90-mCh, Gp93-mCh, mNG-Gp95 include in the virion on the surface of the phage nucleus. None of the five proteins studied enter the phage nucleus. The whole process of assembly of the inner body occurs in the cytoplasm. The obtained results give insight into the assembly of the inner body of giant bacteriophages during the maturation of the viral particles. Localization data of the proteins of the inner body of phiKZ will complement our understanding of functions of phage proteins with low sequence homology.