title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ ÑÑÐ¸Ð¼ÑÐ»ÑÑÐ¸Ð¸ ÑÐÐÐ¤-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾Ð³Ð¾ ÑÐ¸Ð³Ð½Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð¿ÑÑÐ¸ Ð½Ð° Ð¼Ð¸Ð¾Ð³ÐµÐ½Ð½ÑÑ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÑ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ Ð»Ð¸Ð½Ð¸Ð¸ Ð¡2Ð¡12
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: The effect of stimulation of the cAMP-dependent signaling pathway on the myogenic differentiation of the C2C12 cell line
Creators: name: ÐÐ¾Ð¼Ð°ÑÐ¾Ð²Ð°, ÐÐ°ÑÐ³Ð°ÑÐ¸ÑÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: ÑÐ¸ÐºÐ»Ð¸ÑÐµÑÐºÐ¸Ð¹ Ð°Ð´ÐµÐ½Ð¾Ð·Ð¸Ð½Ð¼Ð¾Ð½Ð¾ÑÐ¾ÑÑÐ°Ñ
ÑÐºÐµÐ»ÐµÑÐ½Ð°Ñ Ð¼ÑÑÐºÑÐ»Ð°ÑÑÑÐ°
ÐºÐ»ÐµÑÐ¾ÑÐ½Ð°Ñ Ð»Ð¸Ð½Ð¸Ñ Ð¡2Ð¡12
ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐ¹ ÑÐ¸Ð³Ð½Ð°Ð»Ð¸Ð½Ð³
Ð¼Ð¸oÐ³ÐµÐ½ÐµÐ·
ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð³ÐµÐ½Ð¾Ð²
ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐ¹ Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¼
cyclic adenosine monophosphate
skeletal muscle
Ð¡2Ð¡12 cell line
cell signaling
myogenesis
gene expression
cell metabolism

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð²ÑÑÐ²Ð»ÐµÐ½Ð¸Ñ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½ÑÑ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð¾Ð², Ð¾Ð±ÑÑÐ»Ð°Ð²Ð»Ð¸Ð²Ð°ÑÑÐ¸Ñ ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð°Ð»ÑÐ½ÑÐµ Ð¸ Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸ÑÐµÑÐºÐ¸Ðµ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð² ÑÐºÐµÐ»ÐµÑÐ½Ð¾Ð¹ Ð¼ÑÑÐºÑÐ»Ð°ÑÑÑÐµ Ð¿ÑÐ¸ Ð°ÐºÑÐ¸Ð²Ð°ÑÐ¸Ð¸ ÑÐÐÐ¤-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾Ð³Ð¾ ÑÐ¸Ð³Ð½Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð¿ÑÑÐ¸ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ IBMX. Ð ÑÐ¾Ð´Ðµ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð¸Ñ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð¼ÐµÑÐ¾Ð´Ñ: ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ Ð»Ð¸Ð½Ð¸Ð¸ Ð¡2Ð¡12, Ð¼Ð¸Ð¾Ð³ÐµÐ½Ð½Ð°Ñ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐ° Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Â«Ð³Ð¾Ð»Ð¾Ð´Ð½Ð¾Ð¹ ÑÑÐµÐ´ÑÂ» (HS) Ð¸ IBMX (Ð½ÐµÑÐµÐ»ÐµÐºÑÐ¸Ð²Ð½ÑÐ¹ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾Ñ ÑÐ¾ÑÑÐ¾Ð´Ð¸ÑÑÑÐµÑÐ°Ð·), Ð¸Ð¼Ð¼ÑÐ½Ð¾ÑÐ¸ÑÐ¾ÑÐ¸Ð¼Ð¸ÑÐµÑÐºÐ¾Ðµ Ð¾ÐºÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ðµ, ÐÐ¦Ð  Ð² ÑÐµÐ¶Ð¸Ð¼Ðµ ÑÐµÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð²ÑÐµÐ¼ÐµÐ½Ð¸, Ð²ÐµÑÑÐµÑÐ½-Ð±Ð»Ð¾ÑÑÐ¸Ð½Ð³, Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð±Ð¸Ð¾ÑÐ½ÐµÑÐ³ÐµÑÐ¸ÐºÐ¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð¼Ð¸Ð¾ÑÑÑÐ±Ð¾Ðº Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¸ Seahorse. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð¸Ñ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¾ Ð²ÑÑÐ²Ð»ÐµÐ½Ð¾, ÑÑÐ¾ Ð°ÐºÑÐ¸Ð²Ð°ÑÐ¸Ñ ÑÐÐÐ¤-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾Ð³Ð¾ Ð¿ÑÑÐ¸ Ð¸Ð½Ð´ÑÑÐ¸ÑÑÐµÑ Ð¼Ð¸Ð¾Ð³ÐµÐ½Ð½ÑÑ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÑ Ð¸ ÑÐ»Ð¸ÑÐ½Ð¸Ðµ Ð¼Ð¸Ð¾Ð±Ð»Ð°ÑÑÐ¾Ð², Ð½Ð¾ Ð¿ÑÐ¸ ÑÑÐ¾Ð¼ ÑÐµÐ½Ð¾ÑÐ¸Ð¿ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð¼ÑÑÐµÑÐ½ÑÑ ÑÑÑÐ±Ð¾Ðº Ð¾ÑÐ»Ð¸ÑÐ°ÐµÑÑÑ Ð¾Ñ HS-Ð¼Ð¸Ð¾ÑÑÑÐ±Ð¾Ðº. ÐÐ¾Ð·Ð´ÐµÐ¹ÑÑÐ²Ð¸Ðµ IBMX Ð½Ð° ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ ÐºÑÐ»ÑÑÑÑÑ Ð¡2Ð¡12 Ð¿ÑÐ¸Ð²Ð¾Ð´Ð¸Ñ Ðº Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ ÑÑÐ¾Ð²Ð½Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð², ÑÐµÐ³ÑÐ»Ð¸ÑÑÑÑÐ¸Ðµ Ð¼Ð¸Ð¾Ð³ÐµÐ½ÐµÐ·, Ð¼ÑÑÐµÑÐ½ÑÑ Ð¼Ð¸Ð¾Ð·Ð¸Ð½Ð¾Ð², Ð¿ÑÐ¾Ð¼ÐµÐ¶ÑÑÐ¾ÑÐ½ÑÑ ÑÐ¸Ð»Ð°Ð¼ÐµÐ½ÑÐ¾Ð² Ð¸ Ð³ÐµÐ½Ð¾Ð², ÑÐµÐ³ÑÐ»Ð¸ÑÑÑÑÐ¸Ñ ÑÐ»Ð¸ÑÐ½Ð¸Ðµ Ð¼Ð¸ÑÐ¾ÑÐ¾Ð½Ð´ÑÐ¸Ð¹. ÐÐ½Ð°Ð»Ð¸Ð· ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¼Ð° Ð²ÑÑÐ²Ð¸Ð» ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ðµ Ð¾Ð±ÑÐµÐ³Ð¾ Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð° Ð² IBMX-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ Ð¼ÑÑÐµÑÐ½ÑÑ ÑÑÑÐ±ÐºÐ°Ñ.
description: This work is devoted to the identification of molecular mechanisms that determine functional and metabolic changes in skeletal muscles during activation of a cAMP-dependent signaling pathway using IBMX. In the given work the following methods were used: the C2C12 cell line cultivation, myogenic differentiation using a âHorse serumâ (HS) and IBMX (non-selective inhibitor of phosphodiesterases), immunocytochemical staining, real-time PCR, Western Blotting, cellular bioenergetics analysis using Seahorse technology. As a result of the work, it was revealed that activation of the cAMP-dependent pathway induces myogenic differentiation and fusion of myoblasts. But phenotype of these myotubes was different from HS-induced myotubes. The effect of IBMX on C2C12 cells leads to changes in the gene expression of factors that regulate myogenesis. Also, it affects the myosin expression, intermediate filaments, and genes that regulate mitochondrial fusion. Analysis of cell metabolism revealed a decrease metabolic potential in IBMX-induced muscle tubes.