Additional file 1 of A novel rubber tree PR-10 protein involved in host-defense response against the white root rot fungus Rigidoporus microporus
Creators: name: Longsaward, Rawit
name: Pengnoo, Ashara
name: Kongsawadworakul, Panida
name: Viboonjun, Unchera
Publisher: name: figshare
Year: 2024
Subjects: subject: Plant Biology
subject: FOS: Biological sciences

description: Additional file 1: Supplementary Figure 1. The phenotypes of mock- and R. microporus-inoculated rubber tree. Supplementary Figure 2. Raw Image of One-dimensional (1D) SDS-PAGE gel showed the comparison of the protein patterns of rubber tree leaves after R. microporus inoculation by SDS-PAGE with Coomassie brilliant blue (CBB) dye. Lane 1: protein marker; Lanes 2, 4, 6: mock inoculation at 10, 30, and 50 days after inoculation (DAI), respectively; Lanes 3, 5, 7: R. microporus-inoculation at 10, 30, and 50 DAI, respectively. Supplementary Figure 3. Raw Image of Two-dimensional (2D) SDS-PAGE gels. The three replicates of 2D SDS-PAGE of each treatment are showed below. The framed gels are the representative gels of each treatment. Supplementary Figure 4. Location of the coding sequence of the uncharacterized protein LOC110748447 isoforms on whole genome shotgun contig LVXX01001672.1 (Range: 61922-78548) Hevea brasiliensis cultivar reyan7-33-97 scaffold1672, whole genome shotgun sequence (matched 100% +/- ). Supplementary Figure 5. Location of the coding sequence of the uncharacterized protein LOC110748447 isoforms on whole genome shotgun contig MKXE01009552.1 (Range: 38570-39259) Hevea brasiliensis cultivar RRIM600, whole genome shotgun sequence (matched 100% +/- with isoform X1) The 3000 bp of 5’upstream region was the blue region. Supplementary Figure 6. Location of predicted cis-acting elements on the promoter region of Uncharacterized protein LOC110648447. Supplementary Figure 7. The secondary structure prediction of Uncharacterized protein LOC110648447 isoforms using PSIPRED server. Supplementary Figure 8. The secondary structure prediction of Uncharacterized protein LOC110648447 isoforms using I-TASSER server. Supplementary Figure 9. The predicted normalized B-factor of predicted secondary structure of Uncharacterized protein LOC110648447 isoforms using I-TASSER server. Supplementary Table 1. Identification results of the selected 1D-PAGE band and 2D-PAGE spots by ESI-QUAD-TOF mass spectrometry (MS/MS) analysis. Supplementary Table 2. Location of predicted cis-acting elements on the promoter region of Uncharacterized protein LOC110648447. Supplementary Table 3. The top 10 threading templates used by I-TASSER in predicting the 3D structures of Uncharacterized protein LOC110648447 isoforms. Supplementary Table 4. The alignment of Uncharacterized protein LOC110648447 isoforms with the top 10 threading templates in supplementary table 3. Supplementary Table 5. The top 5 final models of Uncharacterized protein LOC110648447 isoforms predicted by I-TASSER server. Supplementary Table 6. The top 10 structurally closed proteins in the PDB to the model of Uncharacterized protein LOC110648447 isoforms predicted by I-TASSER server. Supplementary Table 7. The predicted functions of Uncharacterized protein LOC110648447 isoforms by COFACTOR and COACH through I-TASSER server.