Poplar Response to Salt Stress
Creators: name: Chayma Assabbane
Publisher: name: Zenodo
Year: 2023

description: This research project aims to identify genes (specifically the NACs family) involved in salt stress tolerance in Populus, a genus of trees known for its ecological amplitude, rapid growth, and ease of cloning. To achieve this, transcriptomic data will be analyzed, and transgenic overexpression constructs will be generated and used to generate poplar lines for phenotypic and physiological analysis using traditional cloning and CRISPR/Cas9 gene editing. The molecular mechanisms of the identified genes and their gene network in response to salt stress will be studied. Tobacco and Arabidopsis thaliana will be used as herbaceous plant models. The project will involve various molecular techniques, including nucleic acid extraction, PCR and electrophoresis, protein analysis, and statistical analysis using ANOVA system. Morphological analysis and stress tolerance assays of transgenic plants will also be conducted. This study aims to contribute to the understanding of the response of Populus trees to salt stress and to identify potential targets for genetic modification to enhance their tolerance<strong>.</strong>
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