Additional file 1 of Activation of embryonic/germ cell-like axis links poor outcomes of gliomas
Creators: name: Ma, Zhan
name: Zhang, Fengyu
name: Xiong, Ji
name: Zhang, Haishi
name: Lin, Hui-Kuan
name: Liu, Chunfang
Publisher: name: figshare
Year: 2022
Subjects: subject: Genetics
subject: FOS: Biological sciences

description: Additional file 1: Figure S1. PCR showed the DAZL withheterozygote (arrow). Figure S2. (A) Immunofluorescence assays showed the expression of DAZLin cultured A172mut cells. (B) AP staining showing PGC-like cells inA172mut glioma cells. (C) Thecomparison of indicated genes between A172 bottom cells and A172 upper cells Scale bar=25 μm. Figure S3. Increase expression of genes related to embryonic/germ cell developmentin gliomas versus normal brain tissues. *P&lt;0.01. Figure S4. Associationbetween the expression of genes related to embryonic/germ cell development andthe pathologic grades/outcomes of gliomas. (A) RNA sequencing showing the mRNA expressionprofiles of the indicated genes in subtypes of gliomas that had different pathologicgrades. (B) RNA array showing the mRNA expression profile of DAZL in subtypesof gliomas that had different pathologic grades. Figure S5. Association between the expression of genes related to embryonic/germcell development and the pathologic grades/outcomes of gliomas. (A) RNAsequencing and clinical data showing the mRNA expression profiles of the genesrelated to IPS reprogramming in gliomas that had different pathologic gradesand outcomes. (B) RNA sequencing and clinical data showing the mRNA expressionprofiles of the genes related to IPS reprogramming in gliomas that haddifferent pathologic grades and outcomes. (C) RNA array showing the mRNAexpression profile of DAZL in gliomas that had different pathologic grades. Figure S6. Association between the expression of genes related to pluripotency andthe pathologic grades/outcomes of gliomas. (A) RNA sequencing and clinicaldata showing the mRNA expression profiles of the indicated genes in gliomasthat had different pathologic grades and outcomes. Figure S7. Associationbetween the mRNA levels of various genes and 1p/19q codeletion status ingliomas.(A) RNA sequencing and clinical data showing the relationship between the mRNAlevel of the indicated genes and 1p/19q codeletion status. (B) The ratio ofgliomas with 1p/19q codeletion to gliomas without 1p/19q codeletion and the meanmRNA values of various genes. Figure S8. Clinicalsignificance and prevalence of molecular groups among gliomas. (A) Patients with the same pathological gradeseparated by the mRNA level of genes in the gene group showed different overallsurvival. Figure S9. Appearance ofgerm cell-like cells in human glioma tissues. (A) Morphology and marker expression of embryonic/germ cell-like cellsin human glioma tissues.(B) HE stainingand Immunohistochemistry assays showing the germ cell-like celland embryolike structures at different developmental stages. Table S1. Sequencing data of CRIPSR-Cas9 knockout glioma cells. Table S2. Detailed information fromthe CGGA database regarding mRNA sequencing. Table S3. Comparison of geneexpression in subtype of gliomas with distinct WHO classification (Unpaired ttest with Welch's correction). Table S4. Comparison of gene expression in gliomas withdistinct WHO classifications (Unpaired t test with Welch's correction and MannWhitney test). Table S5. Comparison of the mediansurvival of gliomas with distinct gene expression. Table S6. Comparison of geneexpression in gliomas with distinct 1p19q codeletion statue (Unpaired t testwith Welch's correction). Table S7. RNA sequencing cut-offvalues of the genes in the molecular group. Table S8. Comparisonof the median survival of gliomas with distinct classifications. Table S9. Positive ratio ofmolecular groups in distinct glioma types. Table S10. Comparison of the median survivalof gliomas with the WHO classification. Table S11.Comparison of the median survival of gliomas with the WHO classification. Table S12. Expression ratio ofproteins related to embryonic/germ cells in glioma tissues.