title: ÐÐ¾Ð»ÑÑÐµÐ½Ð¸Ðµ Ð¸ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° Ð³ÐµÑÐµÑÐ¾ÑÐµÑÑÐ°Ð¼ÐµÑÐ½Ð¾Ð³Ð¾ ÑÐµÐºÐ¾Ð¼Ð±Ð¸Ð½Ð°Ð½ÑÐ½Ð¾Ð³Ð¾ ÑÑÑÐµÐ¿ÑÐ°Ð²Ð¸Ð´Ð¸Ð½Ð°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Preparation and properties of heterotetrameric recombinant streptavidin
Creators: name: ÐÐ¾Ð·Ð»Ð¾Ð²ÑÐºÐ°Ñ, ÐÐ½Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: ÑÑÑÐµÐ¿ÑÐ°Ð²Ð¸Ð´Ð¸Ð½
Ð³ÐµÑÐµÑÐ¾ÑÐµÑÑÐ°Ð¼ÐµÑ
ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÐµ ÑÑÐ»Ð¾Ð²Ð¸Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ
pelb-Ð»Ð¸Ð´ÐµÑÐ½ÑÐ¹ Ð¿ÐµÐ¿ÑÐ¸Ð´
streptavidin
heterotetramer
various cultivation conditions
pelb-leader peptide

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description: This work is devoted to the study of heterotetrameric streptavidine. The purpose of this work is to demonstrate the possibility of obtaining recombinant heterotetrameric protein in E. coli by simultaneous transfusion of bacteria by two plasmids. In this case, one of the plasmids is responsible for the synthesis of a protein monomer with its predominant accumulation in the inclusion bodies, and the second plasmid is responsible for the synthesis of a protein monomer and its transport into the periplasm. In this book methods of experimental research have been developed: creation of a protein expression of the genetic structure, the study of optimal growth conditions of cell culture for the synthesis of the target protein, the purification of the target protein, including protein electrophoresis in PAAG, DNA isolation, restriction, ligation, protein isolation from culture media, a soluble fraction and inclusion bodies and the periplasm. As vectors for the expression of streptavidine, pet22b(+) and ptrc 99A plasmids and E. strains were used. coli BL21 (DE3) were used to produce bacterial mass. Heterotetrameric streptavin is localized in different fractions of E. coli depending on the temperature regime of induction of protein synthesis and IPTG. At a Injection temperature of 26Â°Ð¡, heterotetrameric streptavidine is localized in a significant amount in the culture medium and periplasma. At an induction temperature of 16os, heteroteramic streptavidine is localized in a significant amount in the soluble fraction of the cell. The results of the research can be used as a basis for the development of new more effective methods for the development of monovalent streptavidin for its subsequent use in medicine and science, and can also be used as a basis for the development of a mutation in the TTR gene for patients with transtyretin amyloidosis.