title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ Ð¼Ð¸ÐºÑÐ¾ÑÐµÐ»ÑÐµÑÐ° Ð¸Ð· ÑÐ¸Ð±ÑÐ¸Ð»Ð» ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ð° I ÑÐ¸Ð¿Ð° Ð½Ð° ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ Ðº Ð¾ÑÑÐµÐ¾Ð³ÐµÐ½Ð½Ð¾Ð¹ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐµ Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: The influence of microrelief from type I collagen fibrils on the osteogenic differentiation of mesenchymal human stem cells
Creators: name: Ð¡Ð¾Ð»Ð¾Ð²ÑÐµÐ²Ð°, ÐÐ½Ð°ÑÑÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: ÑÐºÐ°Ð½ÐµÐ²Ð°Ñ Ð¸Ð½Ð¶ÐµÐ½ÐµÑÐ¸Ñ ÐºÐ¾ÑÑÐ½Ð¾Ð¹ ÑÐºÐ°Ð½Ð¸
Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÐµ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸
Ð¾ÑÑÐµÐ¾Ð³ÐµÐ½Ð½Ð°Ñ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐ°
ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½ I ÑÐ¸Ð¿Ð°
Ð¼Ð¸ÐºÑÐ¾ÑÐµÐ»ÑÐµÑÑ
Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½ÑÐ¹ ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½
ÑÐ¸Ð±ÑÐ¸Ð»Ð»ÑÑÐ½ÑÐ¹ ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½
bone tissue engineering
mesenchymal stem cells
osteogenic differentiation
type I collagen
microrelief
molecular collagen
fibrillar collagen

description: ÐÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ðµ Ð¼Ð¸ÐºÑÐ¾ÑÐµÐ»ÑÐµÑÐ¾Ð² Ð¸Ð· Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾Ð¹ Ð¸ ÑÐ¸Ð±ÑÐ¸Ð»Ð»ÑÑÐ½Ð¾Ð¹ ÑÐ¾ÑÐ¼ ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ð° I ÑÐ¸Ð¿Ð° Ð¿Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð½Ð° ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ Ðº Ð¾ÑÑÐµÐ¾Ð³ÐµÐ½Ð½Ð¾Ð¹ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐµ ÐºÐ»ÐµÑÐ¾Ðº Ñ ÑÐ°Ð·Ð½ÑÐ¼ Ð¾ÑÑÐµÐ¾Ð³ÐµÐ½Ð½ÑÐ¼ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð¾Ð¼ (Ð»Ð¸Ð½Ð¸Ð¹ FetMSC, MSC WJ-1, Hos, MG63, Ð¿ÐµÑÐ²Ð¸ÑÐ½ÑÑ ÐºÑÐ»ÑÑÑÑ Ð¾ÑÑÐµÐ¾Ð±Ð»Ð°ÑÑÐ¾Ð² ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð¸ Ð¼ÐµÐ·ÐµÐ½ÑÐ¸Ð¼Ð½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð¿ÑÐ¿Ð¾ÑÐ½Ð¾Ð³Ð¾ ÐºÐ°Ð½Ð°ÑÐ¸ÐºÐ°). ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ñ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ñ ÐºÐ»ÐµÑÐ¾Ðº, ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐµÑÐºÐ¸Ðµ ÑÑÑÑÐºÑÑÑÑ Ð°ÐºÑÐ¸Ð½Ð¾Ð²Ð¾Ð³Ð¾ ÑÐ¸ÑÐ¾ÑÐºÐµÐ»ÐµÑÐ° (Ð½Ð° ÐºÐ»ÐµÑÐºÐ°Ñ Ð»Ð¸Ð½Ð¸Ð¹ FetMSC Ð¸ MSC WJ-1), Ð¸Ð½ÑÐµÐ½ÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð²ÑÑÐ°Ð±Ð¾ÑÐºÐ¸ ÑÐµÐ»Ð¾ÑÐ½Ð¾Ð¹ ÑÐ¾ÑÑÐ°ÑÐ°Ð·Ñ Ð¸ Ð½Ð°ÐºÐ¾Ð¿Ð»ÐµÐ½Ð¸Ñ ÑÐ¾ÑÑÐ°ÑÐ¾Ð² ÐºÐ°Ð»ÑÑÐ¸Ñ. ÐÑÑÐ²Ð»ÐµÐ½Ð¾, ÑÑÐ¾ ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ð¾Ð²ÑÐµ Ð¼Ð¸ÐºÑÐ¾ÑÐµÐ»ÑÐµÑÑ ÑÐ²ÐµÐ»Ð¸ÑÐ¸Ð²Ð°ÑÑ ÑÐ°ÑÐ¿Ð»Ð°ÑÑÐ°Ð½Ð½Ð¾ÑÑÑ ÐºÐ»ÐµÑÐ¾Ðº, ÑÐ¿Ð¾ÑÑÐ´Ð¾ÑÐ¸Ð²Ð°ÑÑ ÑÑÑÑÐºÑÑÑÑ Ð°ÐºÑÐ¸Ð½Ð¾Ð²Ð¾Ð³Ð¾ ÑÐ¸ÑÐ¾ÑÐºÐµÐ»ÐµÑÐ° Ð½Ð° ÑÐ°Ð½Ð½Ð¸Ñ ÑÑÐ°Ð¿Ð°Ñ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐ¸, ÑÑÐ¸Ð»Ð¸Ð²Ð°ÑÑ Ð²ÑÑÐ°Ð±Ð¾ÑÐºÑ ÑÐµÐ»Ð¾ÑÐ½Ð¾Ð¹ ÑÐ¾ÑÑÐ°ÑÐ°Ð·Ñ Ð¸ Ð¿Ð¾ÑÐ²Ð»ÐµÐ½Ð¸Ðµ ÑÐ¾ÑÑÐ°ÑÐ¾Ð² ÐºÐ°Ð»ÑÑÐ¸Ñ (Ð¿ÑÐ¸Ð·Ð½Ð°ÐºÐ¸ Ð¾ÑÑÐµÐ¾Ð³ÐµÐ½Ð½Ð¾Ð¹ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐ¸ ÐºÐ»ÐµÑÐ¾Ðº). ÐÐ»Ñ Ð»Ð¸Ð½Ð¸Ð¹ ÐÐ¡Ð ÑÑÐ¸ Ð¿ÑÐ¸Ð·Ð½Ð°ÐºÐ¸ Ð±ÑÐ»Ð¸ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ Ð²ÑÑÐ°Ð¶ÐµÐ½Ñ Ð¿ÑÐ¸ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ Ð½Ð° Ð¼Ð¸ÐºÑÐ¾ÑÐµÐ»ÑÐµÑÐµ Ð¸Ð· ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ð° Ð² Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾Ð¹ ÑÐ¾ÑÐ¼Ðµ.
description: The influences of the type I collagen molecular and fibrillar form microreliefs on the osteogenic differentiation of cells with different osteogenic potential (FetMSC, MSC WJ-1, Hos, MG63 lines, primary cultures of human osteoblasts and umbilical cord mesenchymal stem cells) were compared. The cell morphology, the actin cytoskeleton structures (FetMSC and MSC WJ-1 cells), the intensity of alkaline phosphatase and calcium phosphates production were studied. It was revealed that collagen microreliefs promote cell spreading, arrangement of the actin cytoskeleton structures in the early stages of differentiation, increase in alkaline phosphatase production and the accumulation of calcium phosphates (signs of osteogenic cell differentiation). For MSC lines, the most prominent effect was discovered when cultured on a microrelief from molecular form of collagen.