Additional file 1 of RUNX1, FUS, and ELAVL1-induced circPTPN22 promote gastric cancer cell proliferation, migration, and invasion through miR-6788-5p/PAK1 axis-mediated autophagy
Creators: name: Ma, Shuo
name: Xu, Yanhua
name: Qin, Xinyue
name: Tao, Mei
name: Gu, Xinliang
name: Shen, Lei
name: Chen, Yinhao
name: Zheng, Ming
name: Qin, Shiyi
name: Wu, Guoqiu
name: Ju, Shaoqing
Publisher: name: figshare
Year: 2024
Subjects: subject: Biophysics
subject: Biochemistry
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Molecular Biology
subject: Physiology
subject: Chemical Sciences not elsewhere classified
subject: Immunology
subject: FOS: Clinical medicine
subject: Developmental Biology
subject: Cancer
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Computational Biology

description: Additional file 1: Table S1: The primer sequence of qRT-PCR. Table S2: Plasmid sequences were transfected. Table S3 Bioinformatics analysis was used to analyze the specific information. Table S4 Seven sites where RUNX1 binds to the promoter region of PTPN22. Figure S1. circPTPN22 can promote the proliferation, migration, and invasion of GC cells. A and B. qRT-PCR detection of circPTPN22 knockdown and overexpression efficiency in GC cells. C-H. The effect of knockdown or overexpression of circPTPN22 on the proliferation of GC cells was detected by cck-8 assay (C and D) and EdU assay (E–H). I-P. The effects of knockdown or overexpression of circPTPN22 on migration (I-L) and invasion (M-P) of GC cells were detected by transwell assay. **p &lt; 0.01, ***p &lt; 0.001, ****p &lt; 0.001. Figure S2. Evidence for circPTPN22 binding to miR-6788-5p. A and B. Bioinformatics analysis of RBPs, IRES sites and ORF reading frames that circPTPN22 may bind. C. Expression of miR-6788-5p in GC cells. D. Survival analysis of GC patients in miR-6788-5p high and low groups. *p &lt; 0.05, **p &lt; 0.01, ****p &lt; 0.001. Figure S3. miR-6788-5p inhibited the proliferation, migration, and invasion of GC cells. A-E. Using cck-8 (A), cell colony formation assay (B and C), and EdU cell proliferation assay (D and E) to detect the effect of adding miR-6788-5p inhibitor or mimic on the proliferation of GC cells. F and G. Transwell assay was used to detect the effect of adding miR-6788-5p inhibitor or mimic on the migration and invasion of GC cells. H. Western blot detection of E-cad and vimentin protein levels in GC cells after adding miR-6788-5p inhibitor or mimic. **p &lt; 0.01, ***p &lt; 0.001, ****p &lt; 0.001. Figure S4. The expression level of PAK1 in GC cells. *p &lt; 0.05, ****p &lt; 0.001. Figure S5. FUS and ELAVL1 can partially restore the effects of circPTPN22 on the proliferation, migration, and invasion of GC cells. A. The expression level of RUNX1 in gastric cancer tissues. B. The expression level of FUS and ELAVL1 in GC tissues. C. Knockdown efficiency of FUS and ELAVL1 in GC cells. D-F. After the knockdown of FUS or ELAVL1, the overexpression plasmid of circPTPN22 was transfected, and the proliferation of GC cells was detected by cck-8 (D) and cell colony formation assay (E and F). G-J. After the knockdown of FUS or ELAVL1, the overexpression plasmid of circPTPN22 was transfected, and the migration (G and H) and invasion (I and J) abilities of GC cells were detected by transwell assay. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001, ****p &lt; 0.001. Supplementary file- the full uncropped Gels and Blots image