title: The effect of pH on the collagen fibril forming ability and the mesenchymal stromal cell viability in vitro
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ ÑH Ð½Ð° ÑÐ¸Ð±ÑÐ¸Ð»Ð»Ð¾Ð±ÑÐ°Ð·ÑÑÑÑÑ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ð° Ð¸ Ð¶Ð¸Ð·Ð½ÐµÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÑ ÑÑÑÐ¾Ð¼Ð°Ð»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº in vitro
Creators: name: ÐÐ°Ð²Ð°Ñ Ð§Ð°Ð²ÐµÐ·, Ð­Ð´Ð¸ÑÐ¾Ð½
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: ÐÐ»ÐµÑÐºÐ° (Ð±Ð¸Ð¾Ð».)
ÐÐ¸Ð¾ÑÐµÑ Ð½Ð¾Ð»Ð¾Ð³Ð¸Ñ
ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½ 1 ÑÐ¸Ð¿Ð°
ÑÐºÐ°Ð½Ð¸ÑÑÑÑÐ°Ñ ÑÐ»ÐµÐºÑÑÐ¾Ð½Ð½Ð°Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ
type 1 collagen
scanning electron microscopy

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ pH ÑÐ°ÑÑÐ²Ð¾ÑÐ° ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ð° Ð½Ð° ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ Ð±ÐµÐ»ÐºÐ° Ð¾Ð±ÑÐ°Ð·Ð¾Ð²ÑÐ²Ð°ÑÑ ÑÐ¸Ð±ÑÐ¸Ð»Ð»Ñ in vitro, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð±Ð¸Ð¾ÑÐ¾Ð²Ð¼ÐµÑÑÐ¸Ð¼Ð¾ÑÑÐ¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ ÑÐ¸Ð±ÑÐ¸Ð»Ð» Ð¿Ð¾ Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ñ Ðº ÐºÐ»ÐµÑÐºÐ°Ð¼ FetMSC. ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ Ð±ÑÐ»Ð¸ ÑÐµÑÐµÐ½Ñ Ð² ÑÐ¾Ð´Ðµ ÑÐ°Ð±Ð¾ÑÑ: 1. ÐÐ¾Ð»ÑÑÐ¸ÑÑ Ð¼Ð°ÑÑÐ¸ÑÑ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ ÑÐ°ÑÑÐ²Ð¾ÑÐ° ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ð° Ñ Ð´ÑÑÐ³Ð¸Ð¼ Ð·Ð½Ð°ÑÐµÐ½Ð¸ÐµÐ¼ pH; 2. ÐÐ·ÑÑÐ¸ÑÑ ÑÑÑÑÐºÑÑÑÐ½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð·; 3. ÐÐ·ÑÑÐ¸ÑÑ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ðµ ÑÑÐµÐµÐº Ñ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÐ¼Ð¸ Ð¼Ð°ÑÑÐ¸ÑÐ°Ð¼Ð¸. Ð ÑÐ°Ð±Ð¾ÑÐµ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ°ÐºÐ¸Ðµ Ð¼ÐµÑÐ¾Ð´Ñ, ÐºÐ°Ðº ÑÐºÐ°Ð½Ð¸ÑÑÑÑÐ°Ñ ÑÐ»ÐµÐºÑÑÐ¾Ð½Ð½Ð°Ñ Ð¸ ÐºÐ¾Ð½ÑÐ¾ÐºÐ°Ð»ÑÐ½Ð°Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð°Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ Ð¸ ÑÐ¿ÐµÐºÑÑÐ¾ÑÐ¾ÑÐ¾Ð¼ÐµÑÑÐ¸Ñ. ÐÐ¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ pH ÑÐ°ÑÑÐ²Ð¾ÑÐ° Ð²Ð»Ð¸ÑÐµÑ Ð½Ð° Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ðµ ÑÐ¸Ð±ÑÐ¸Ð»Ð», Ð° ÑÐ°ÐºÐ¶Ðµ Ð½Ð° Ð¸Ñ ÑÐ°ÑÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð¿Ð¾ Ð¿Ð¾Ð²ÐµÑÑÐ½Ð¾ÑÑÐ¸ Ð¸ Ð´Ð¸Ð°Ð¼ÐµÑÑÑ Ð¿Ð¾Ð´Ð»Ð¾Ð¶ÐºÐ¸. ÐÐ°ÐºÑÐ¸Ð¼Ð°Ð»ÑÐ½ÑÐ¹ Ð´Ð¸Ð°Ð¼ÐµÑÑ ÑÐ¸Ð±ÑÐ¸Ð»Ð» Ð½Ð°Ð±Ð»ÑÐ´Ð°Ð»ÑÑ Ð´Ð»Ñ Ð¾Ð±ÑÐ°Ð·ÑÐ¾Ð², Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð¸Ð· ÑÐ°ÑÑÐ²Ð¾ÑÐ° ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ð° Ñ pH 6. ÐÑÐ¸ Ð¸Ð·ÑÑÐµÐ½Ð¸Ð¸ Ð°Ð´Ð³ÐµÐ·Ð¸Ð¸ ÐºÐ»ÐµÑÐ¾Ðº Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ ÐºÐ¾Ð½ÑÐ¾ÐºÐ°Ð»ÑÐ½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸ Ð±ÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð¼Ð°ÐºÑÐ¸Ð¼Ð°Ð»ÑÐ½Ð¾Ðµ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð¾ ÐºÐ»ÐµÑÐ¾Ðº Ð² Ð¿ÐµÑÐ²ÑÐµ Ð½ÐµÑÐºÐ¾Ð»ÑÐºÐ¾ ÑÐ°ÑÐ¾Ð² Ð¿Ð¾ÑÐ»Ðµ Ð¿Ð¾ÑÐµÐ²Ð° Ð¿ÑÐ¸Ð»Ð¸Ð¿Ð°ÐµÑ Ðº Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð²ÑÐ¸Ð¼ÑÑ ÑÐ¸Ð±ÑÐ¸Ð»Ð»Ð°Ð¼ ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ð°. Ð¸Ð· ÑÐ°ÑÑÐ²Ð¾ÑÐ° ÐºÐ¾Ð»Ð»Ð°Ð³ÐµÐ½Ð° Ñ pH 7. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ Ð¼Ð°ÑÑÐ¸ÑÑ Ð¼Ð¾Ð¶Ð½Ð¾ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°ÑÑ Ð´Ð»Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÐºÐ»ÐµÑÐ¾Ðº Ð¸ Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ ÑÑÐ±ÑÑÑÐ°ÑÐ¾Ð² Ð´Ð»Ñ ÑÑÐ°Ð½ÑÐ¿Ð»Ð°Ð½ÑÐ°ÑÐ¸Ð¸ ÐºÐ»ÐµÑÐ¾Ðº Ð¿Ð¾ÑÐ»Ðµ ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¼Ð¾Ð½Ð¾ÑÐ»Ð¾Ñ Ð½Ð° ÑÐ°Ð½Ð°Ñ Ð´Ð»Ñ ÑÑÐºÐ¾ÑÐµÐ½Ð¸Ñ Ð¿ÑÐ¾ÑÐµÑÑÐ¾Ð² ÑÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸Ð¸.
description: This work is devoted to the study of the effect of the pH of the collagen solution on the ability of the protein to form fibrils in vitro, as well as to the study of the biocompatibility of the obtained fibrils with respect to FetMSC cells. Tasks that were solved during the work: 1. To obtain matrices based on a collagen solution with a different pH value; 2. To study the structural analysis; 3. To study the interaction of cells with the resulting matrices. Methods such as scanning electron and confocal fluorescence microscopy and spectrophotometry were used in the work. It is shown that the pH of the solution affects the formation of fibrils, as well as their distribution on the substrate surface and diameter. The maximum diameter of the fibrils was observed for samples obtained from a collagen solution with a pH of 6. When studying cell adhesion by confocal microscopy, it was shown that the maximum number of cells in the first few hours after sowing adheres to collagen fibrils formed from a collagen solution with a pH of 7. The resulting matrices can be used for cell culture and as substrates for cell transplantation after the formation of a monolayer on wounds to accelerate regeneration processes.