Additional file 1 of A closer look at the WHO cone bioassay: video analysis of the hidden effects of a human host on mosquito behaviour and insecticide contact
Creators: name: Hughes, Angela
name: Matope, Agnes
name: Emery, Mischa
name: Steen, Keith
name: Murray, Gregory
name: Ranson, Hilary
name: McCall, Philip J.
name: Foster, Geraldine M.
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Neuroscience
subject: Physiology
subject: FOS: Biological sciences
subject: Ecology
subject: 111714 Mental Health
subject: FOS: Health sciences

description: Additional file 1: Table S1. Total imprecision assessment of VCT on untreated net by event type (net contact, flight, cone contact) for each mosquito strain (Kisumu, N’gousso, Banfora and VK7) of An. gambiae s.l. in the absence and presence of a host. Where n (mos) = number of mosquitoes per group, n (reps) = number of replicates performed per group, mean = mean number of mosquitoes per event, SD = standard deviation of the mean, LCLM = lower confidence level mean, UCLM = upper confidence level mean, %CV = percentage coefficient of variation, IS = insecticide susceptible, and IR = insecticide resistant. Table S2. Within-day imprecision assessment of VCT on untreated net by event type (net contact, flight, cone contact) for each strain (Kisumu, N’gousso, Banfora and VK7) of An. gambiae s.l. in the absence and presence of a host. Where n (test days) = number of test days per group, n (reps per test day) = number of replicates performed per test day per group, mean = mean number of mosquitoes per event, SD = standard deviation of the mean, %CV = percentage coefficient of variation, IS = insecticide susceptible, and IR = insecticide resistant. Table S3. Variation in test stages. Assessment of VCT on untreated net by event type (net contact only) for each strain (Kisumu, N’gousso, Banfora and VK7) of An. gambiae s.l. in the absence and presence of a host. Where n (reps) = number of replicates performed per group, %CV = percentage coefficient of variation by time (s = seconds), IS = insecticide susceptible, and IR = insecticide resistant. Table S4. Host-Location comparisons within An. gambiae s.l. strain and net treatment. A Beta-binomial Distribution model fitted. Multiple pairwise comparisons 95% Confidence Intervals and P-values corrected using the Bonferroni adjustment. Where IS = insecticide susceptible, IR = insecticide resistant, UT = Untreated net, OS = Olyset net, P2 = PermaNet 2.0 net and * = Significant at 5% significance level. Table S5. Treatment-Location comparisons within An. gambiae strain (Kisumu, N’gousso, Banfora and VK7) and host (present or absent). A Beta-binomial Distribution model Results. Multiple pairwise comparisons 95% Confidence Intervals and P-values corrected using the Bonferroni adjustment. Where IS = insecticide susceptible, IR = insecticide resistant, UT = Untreated net, OS = Olyset net, P2 = PermaNet 2.0 net and * = Significant at 5% significance level. Table S6. Knock-down (KD) (1 h) and mortality (24 h) after net exposure for insecticide susceptible (Kisumu and N’gousso) and insecticide resistant (VK7 and Banfora) strains of An. gambiae s.l. against PermaNet 2.0 (P2) and Olyset (OS) ITNs, in VCT validation experiments. Table S7. Treatment comparisons—Willingness to refeed at 1 or 24 h within An. gambiae strain and host (present or absent). A Binary Logistic Regression model fitted using Generalised Estimating Equation. Where IR = insecticide resistant, UT = Untreated net, OS = Olyset net, P2 = PermaNet 2.0 net and * = Significant at 5% significance level. Table S8. Host comparisons—Willingness to refeed at 1 or 24 h within An. gambiae strain and treatment. A Binary Logistic Regression model fitted using Generalised Estimating Equation. Where IR = insecticide resistant, UT = Untreated net, OS = Olyset net, P2 = PermaNet 2.0 net and * = Significant at 5% significance level. Table S9. Treatment comparisons – Blood meal size within An. gambiae strain and host (present or absent). A Linear Regression model fitted using Generalised Estimating Equation. Where IS = insecticide susceptible, IR = insecticide resistant, UT = Untreated net, OS = Olyset net, P2 = PermaNet 2.0, CI = Confidence Interval net and * = Significant at 5% significance level. Table S10. Host comparisons – Blood meal size within An. gambiae strain and treatment. A Linear Regression model fitted using Generalised Estimating Equation. Where IS = insecticide susceptible, IR = insecticide resistant, UT = Untreated net, OS = Olyset net, P2 = PermaNet 2.0 net and * = Significant at 5% significance level. Table S11. Descriptive statistics for longevity data within host, An. gambiae strain and net treatment. Where BF = Banfora, VK = VK7, UT = Untreated net, P2 = PermaNet 2.0 net, OS = Olyset net, IR = insecticide resistant, n = sample size, % = percentage, SD = Standard Deviation, Min = Minimum, Max = Maximum, CI = Confidence Interval, Yes n = number of mosquitoes that died within 9 days and * results for mosquitoes that died within 9 days post-exposure only. Table S12. Net treatment comparisons—Mortality within 9 days within An. gambiae strain and host. A Weighted Cox Regression Model fitted adjusted for other predictors (time fed, wingspan, haematin and net proportion). Where UT = Untreated net, P2 = PermaNet 2.0 net, OS = Olyset net, IR = insecticide resistant, Ref = Reference group, Net proportion = Mean proportion of mosquitoes on the net during exposure (behaviour data) and * = Significant at 5% significance level. Censoring at 9 days. Table S13. Host comparisons—Mortality within 9 days within An. gambiae strain and net treatment. A Weighted Cox Regression Model fitted adjusted for other predictors (time fed, wingspan, haematin and net proportion). Where UT = Untreated net, P2 = PermaNet 2.0 net, OS = Olyset net, IR = insecticide resistant, Ref = Reference group, Net proportion = Mean proportion of mosquitoes on the net during exposure (behaviour data) and * = Significant at 5% significance level. Censoring at 9 days.