Interaction of roses with a biotrophic and a hemibiotrophic leaf pathogen leads to differences in defense transcriptome activation
Creators: name: Neu, Enzo
name: Domes, Helena Sophie
name: Menz, Ina
name: Kaufmann, Helgard
name: Linde, Marcus
name: Debener, Thomas
Publisher: name: Dordrecht : Springer Netherlands
Year: 2019
Subjects: subject: Black spot
subject: High-throughput qPCR
subject: MACE analysis
subject: Powdery mildew
subject: PR genes
subject: WRKY genes
subject: Arabidopsis protein
subject: chitinase
subject: flavonoid
subject: pathogen associated molecular pattern
subject: phytohormone
subject: plant protein
subject: salicylic acid
subject: transcription factor
subject: transcriptome
subject: WRKY57 protein, Arabidopsis
subject: Ascomycetes
subject: gene expression profiling
subject: gene expression regulation
subject: genetics
subject: high throughput sequencing
subject: host pathogen interaction
subject: immunity
subject: immunology
subject: metabolism
subject: microbiology
subject: pathogenicity
subject: plant disease
subject: plant gene
subject: plant leaf
subject: rose
subject: signal transduction
subject: Arabidopsis Proteins
subject: Ascomycota
subject: Chitinases
subject: Flavonoids
subject: Gene Expression Profiling
subject: Gene Expression Regulation, Plant
subject: Genes, Plant
subject: High-Throughput Nucleotide Sequencing
subject: Host-Pathogen Interactions
subject: Immunity
subject: Pathogen-Associated Molecular Pattern Molecules
subject: Plant Diseases
subject: Plant Growth Regulators
subject: Plant Leaves
subject: Plant Proteins
subject: Rosa
subject: Salicylic Acid
subject: Signal Transduction
subject: Transcription Factors
subject: Transcriptome
subject: Dewey Decimal Classification::500 | Naturwissenschaften::580 | Pflanzen (Botanik)

description: Key message: Transcriptomic analysis resulted in the upregulation of the genes related to common defense mechanisms for black spot and the downregulation of the genes related to photosynthesis and cell wall modification for powdery mildew. Abstract: Plant pathogenic fungi successfully colonize their hosts by manipulating the host defense mechanisms, which is accompanied by major transcriptome changes in the host. To characterize compatible plant pathogen interactions at early stages of infection by the obligate biotrophic fungus Podosphaera pannosa, which causes powdery mildew, and the hemibiotrophic fungus Diplocarpon rosae, which causes black spot, we analyzed changes in the leaf transcriptome after the inoculation of detached rose leaves with each pathogen. In addition, we analyzed differences in the transcriptomic changes inflicted by both pathogens as a first step to characterize specific infection strategies. Transcriptomic changes were analyzed using next-generation sequencing based on the massive analysis of cDNA ends approach, which was validated using high-throughput qPCR. We identified a large number of differentially regulated genes. A common set of the differentially regulated genes comprised of pathogenesis-related (PR) genes, such as of PR10 homologs, chitinases and defense-related transcription factors, such as various WRKY genes, indicating a conserved but insufficient PTI [pathogen associated molecular pattern (PAMP) triggered immunity] reaction. Surprisingly, most of the differentially regulated genes were specific to the interactions with either P. pannosa or D. rosae. Specific regulation in response to D. rosae was detected for genes from the phenylpropanoid and flavonoid pathways and for individual PR genes, such as paralogs of PR1 and PR5, and other factors of the salicylic acid signaling pathway. Differently, inoculation with P. pannosa leads in addition to the general pathogen response to a downregulation of genes related to photosynthesis and cell wall modification. © 2019, The Author(s).