title: Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° ÑÐ¿Ð¾ÑÐ¾Ð±Ð° Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ° Ð¿Ð¸Ð³Ð¼ÐµÐ½ÑÐ¾Ð² Ð¸Ð· Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Vischeria punctata
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Development of a method for obtaining a complex of pigments from Vischeria punctata biomass
Creators: name: Ð§ÐµÑÐµÐ·Ð¾Ð², ÐÐ»ÐµÐºÑÐµÐ¹
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Ð¿Ð¸Ð³Ð¼ÐµÐ½ÑÐ½ÑÐ¹ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑ
Vischeria punctata
Ñ Ð»Ð¾ÑÐ¾ÑÐ¸Ð»Ð»
ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ñ
ÑÐ»ÑÑÑÐ°Ð·Ð²ÑÐºÐ¾Ð²Ð°Ñ ÑÐºÑÑÑÐ°ÐºÑÐ¸Ñ
pigment complex
chlorophyll
carotenoids
ultrasonic extraction

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° ÑÐ¿Ð¾ÑÐ¾Ð±Ð° Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ° Ð¿Ð¸Ð³Ð¼ÐµÐ½ÑÐ¾Ð² Ð¸Ð· Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Vischeria punctataÂ». ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ ÑÑÑÐµÐºÑÐ¸Ð²Ð½ÑÑ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÐ¾Ð² ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸ Ð¿Ð¸Ð³Ð¼ÐµÐ½ÑÐ½Ð¾Ð³Ð¾ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ° Ð¸Ð· Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ð¸ Vischeria punctata. ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: ÑÑÐ°Ð²Ð½Ð¸ÑÑ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÐµ Ð¼ÐµÑÐ¾Ð´Ñ ÑÑÑÐºÐ¸ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Vischeria punctata Ð´Ð»Ñ ÑÐºÑÑÑÐ°Ð³Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¿Ð¸Ð³Ð¼ÐµÐ½ÑÐ½Ð¾Ð³Ð¾ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ°; Ð¾Ð¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ Ð¾Ð¿ÑÐ¸Ð¼Ð°Ð»ÑÐ½ÑÐµ ÑÑÐ»Ð¾Ð²Ð¸Ñ ÑÐ»ÑÑÑÐ°Ð·Ð²ÑÐºÐ¾Ð²Ð¾Ð¹ ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸ Ð¿Ð¸Ð³Ð¼ÐµÐ½ÑÐ½Ð¾Ð³Ð¾ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ°: ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÑ, Ð²ÑÐµÐ¼Ñ ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸; Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°ÑÑ ÑÐµÑÐ¼ÐµÐ½ÑÑ Ð´ÐµÐ·Ð¸Ð½ÑÐµÐ³ÑÐ°ÑÐ¸Ð¸ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ ÑÑÐµÐ½ÐºÐ¸ Ñ ÑÐµÐ»ÑÑ ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ñ Ð²ÑÑÐ¾Ð´Ð° Ð¿Ð¸Ð³Ð¼ÐµÐ½ÑÐ½Ð¾Ð³Ð¾ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ°; ÑÐ¾ÑÑÐ°Ð²Ð¸ÑÑ ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÑÑ ÑÑÐµÐ¼Ñ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ Ð¿Ð¸Ð³Ð¼ÐµÐ½ÑÐ½Ð¾Ð³Ð¾ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ°. ÐÐ±ÑÐµÐºÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ñ Vischeria punctata Vischer, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½Ð°Ñ Ð¸Ð· ÐºÐ¾Ð»Ð»ÐµÐºÑÐ¸Ð¸ ÐÐ°Ð»ÑÐ¸Ð¹ÑÐºÐ¾Ð³Ð¾ ÑÐµÐ´ÐµÑÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑÐ° Ð¸Ð¼ÐµÐ½Ð¸ ÐÐ¼Ð¼Ð°Ð½ÑÐ¸Ð»Ð° ÐÐ°Ð½ÑÐ°, ÑÑÐ°Ð¼Ð¼ IPPAS H-242. Ð Ð°Ð±Ð¾ÑÐ° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ Ð¡ÐÐÐÐ£ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾, ÐÐÐ¡Ð¸Ð, ÐÐ¨ÐÐ¸ÐÐ. ÐÐ»Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ: Â«ÐÐ¾Ð»Ð»ÐµÐºÑÐ¸Ñ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹ Ð¸ ÑÐ¸Ð°Ð½Ð¾Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ IPPAS ÐÐ¤Ð  Ð ÐÐÂ»; Â«Listing the Worldâs Algae AlgaeBaseÂ»; Â«ÐÐ¾Ð»Ð»ÐµÐºÑÐ¸Ñ ÐºÑÐ»ÑÑÑÑ Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹ Ð² ÐÐµÑÑÐ¸Ð½Ð³ÐµÐ½ÑÐºÐ¾Ð¼ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑÐµ (SAG)Â», Ð° ÑÐ°ÐºÐ¶Ðµ ÐµLIBRARY.RU, Scopus. ÐÐ»Ñ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÐ¸ Ð¸ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð´Ð°Ð½Ð½ÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ Ð¿Ð°ÐºÐµÑ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼ Microsoft Exel. ÐÐ»Ñ Ð¼Ð¾ÑÑÐ¾Ð¼ÐµÑÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸ÑÐµÑÐºÐ¾Ð¹ ÐºÐ°ÑÑÐ¸Ð½Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ñ ÐÐ¡-foto Ð¸ Levenhuk. ÐÐ¾ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ°Ð¼ Ð»Ð¸Ð¾ÑÐ¸Ð»ÑÐ½Ð°Ñ ÑÑÑÐºÐ° ÑÐ¿Ð¾ÑÐ¾Ð±ÑÑÐ²ÑÐµÑ Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐµÐ¹ ÑÐ»ÑÑÑÐ°Ð·Ð²ÑÐºÐ¾Ð²Ð¾Ð¹ ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸ Ð¿Ð¸Ð³Ð¼ÐµÐ½ÑÐ½Ð¾Ð³Ð¾ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ° (Ð¸ Ð²ÑÐµÑ ÐµÐ³Ð¾ ÑÑÐ°ÐºÑÐ¸Ð¹) ÑÑÐ°Ð½Ð¾Ð»Ð¾Ð¼, Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð°Ñ ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÐ° ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸ Ð¿Ð¸Ð³Ð¼ÐµÐ½ÑÐ½Ð¾Ð³Ð¾ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ° Ð¾Ñ 45 Ð´Ð¾ 50 Â°Ð¡, Ð´Ð¾ÑÑÐ°ÑÐ¾ÑÐ½Ð¾ 40 Ð¼Ð¸Ð½ÑÑ ÑÐºÑÑÑÐ°Ð³Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ, Ð´Ð»Ñ ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ð¾Ð² 30 Ð¼Ð¸Ð½ÑÑ, Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ðµ ÑÐµÑÐ¼ÐµÐ½ÑÐ¾Ð² Ð½Ð° ÐºÐ¾Ð½ÐµÑÐ½ÑÑ ÑÑÐ°Ð¿Ð°Ñ ÑÐ»ÑÑÑÐ°Ð·Ð²ÑÐºÐ¾Ð²Ð¾Ð¹ ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸ Ð±Ð¾Ð»ÐµÐµ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾, ÑÐµÐ¼ Ð¿ÐµÑÐµÐ´ ÑÐºÑÑÑÐ°Ð³Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸ÐµÐ¼. Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð½ÑÐ¹ ÑÐ¿Ð¾ÑÐ¾Ð± Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÐµÑ Ð¿Ð¾Ð»ÑÑÐ¸ÑÑ 36,47 Â± 0,52 Ð¼Ð³ Ð¿Ð¸Ð³Ð¼ÐµÐ½ÑÐ¾Ð² (15,09 Â± 0,22 % ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ð¾Ð²) Ð¸Ð· Ð¾Ð´Ð½Ð¾Ð³Ð¾ Ð»Ð¸ÑÑÐ° ÑÑÑÐ¿ÐµÐ½Ð·Ð¸Ð¸ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½Ð¾Ð¹ Ð² ÑÐµÑÐµÐ½Ð¸Ðµ 7 ÑÑÑÐ¾Ðº ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ.
description: The subject of the graduate qualification work is "Development of a method for obtaining a complex of pigments from Vischeria punctata biomass". This work is devoted to determining the most effective parameters for the extraction of a pigment complex from the biomass of the microalgae Vischeria punctata. The tasks that were solved during the study is to compare different methods of drying Vischeria punctata biomass for extracting the pigment complex; to determine the optimal conditions for ultrasonic extraction of the pigment complex: temperature, extraction time; to use cell wall disintegration enzymes in order to increase the yield of the pigment complex; to draw up a technological scheme for obtaining the pigment complex. Object of research is the microalgae Vischeria punctata Vischer obtained from the collection of the Immanuel Kant Baltic Federal University, strain IPPAS H-242. The work carried out at Peter the Great SPBPU, IBSB, HSBFP. The following databases were used for the study: "Collection of microalgae and Cyanobacteria IPPAS IGF RAS"; "Listing the Worlds Algae AlgaeBase"; "Collection of algae cultures at the University of GÃ¶ttingen (SAG)", as well as Library.RU, Scopus. Microsoft Excel software package used for data processing and analysis. The MS-foto and Levenhuk programs used for morphometric examination of the microscopic picture. According to the results, lyophilic drying promotes further ultrasonic extraction of the pigment complex (and all its fractions) with ethanol, the most effective extraction temperature of the pigment complex is 45 ï­ 50 Â°C, 40 minutes of extraction is sufficient, 30 minutes for carotenoids, the use of enzymes at the final stages of ultrasonic extraction is more effective than before extraction. The developed method makes it possible to obtain 36.47 Â± 0.52 mg of pigments (15.09 Â± 0.22 % carotenoids) from one liter of microalgae suspension obtained during 7 days of cultivation.