Melomastia puerensis R. F. Xu & Tibpromma, MycoKeys
Creators: name: Zhang, Xian
name: Tibpromma, Saowaluck
name: Karunarathna, Samantha C.
name: Du, Tian-Ye
name: Han, Li-Su
name: Elgorban, Abdallah M.
name: Kumla, Jaturong
name: Senwanna, Chanokned
name: Dai, Dong-Qin
name: Suwannarach, Nakarin
name: Wang, Hao-Han
Publisher: name: Zenodo
Year: 2025
Subjects: Biodiversity
Taxonomy
Fungi
Ascomycota
Dothideomycetes
Botryosphaeriales
Botryosphaeriaceae
Melomastia
Melomastia puerensis

description: Melomastia puerensis R. F. Xu & Tibpromma, MycoKeys 103: 75 (2024)
Fig. 8
Holotype.
ZHKU 23-0106.
Description.
Saprobic on dead twigs of Macadamia integrifolia. Sexual morph: Ascomata 300–650 × 430–605 µm (x ̄ = 521 × 516 µm, n = 20), visible as black raised dots on the host surface, solitary, semi-immersed, dark brown to black, subglobose or irregular pyriform, carbonaceous, papillate. Ostiolar central, carbonaceous, brown to dark brown to black. Peridium 30–80 µm wide (x ̄ = 52 µm, n = 25), comprising two section layers: inner section layers hyaline to brown cells of textura prismatica, outer section layer, brown to black cells of textura prismatica. Hamathecium 2–4 µm wide (x ̄ = 3 μm, n = 60), hyaline, filiform, septate, branched, pseudoparaphyses, longer than asci. Asci 166–235 × 5.6–9.9 µm (x ̄ = 196 × 8.1 µm, n = 30), 8 - spored, bitunicate, cylindrical, flexuous, smooth-walled, apically obtuse, with an ocular chamber, short-pedicellate. Ascospores 19–30 × 5–8 µm (x ̄ = 26 × 6.5 µm, n = 50), hyaline, fusiform, uniseriate, 3 - septate, narrow towards the apex and obtuse or conical ends, constricted at the septa, smooth-walled, without mucilaginous sheath or appendages, with guttules in each cell. Asexual morph: Undetermined.
Material examined.
China • Yunnan Province, Baoshan City, 24°48'18"N, 99°22'36"E, 1199.6 m elevation, on a dead branch of Macadamia integrifolia, 30 July 2022, Xian Zhang, MBC 85, GMB 1173, GMB 1174, new host record.
GenBank number.
GMB 1173 = LSU: PQ 669573, SSU: PQ 669629, tef 1 - α: PQ 685655; GMB 1174 = LSU: PQ 669574, SSU: PQ 669630, tef 1 - α: PQ 685656.
Known host and distribution.
On a dead branch of Hevea brasiliensis in China (Xu et al. 2024), on a dead branch of Macadamia integrifolia in China (this study).
Notes.
Macadamia puerensis was reported by Xu et al. (2024), who isolated it from Hevea brasiliensis (Willd. ex A. Juss.) Müll. Arg., in Pu’er City, Yunnan Province, China. In the phylogenetic analyses, our strain M. puerensis (GMB 1173) clustered with M. puerensis (ZHKUCC 23–0802, ex-type) with 100 % ML and 1.00 PP support (Fig. 7). The nucleotide comparisons showed that our strain is not significantly different from ZHKUCC 23–0802 in LSU (0 / 842 bp (0 %), without gaps), SSU (3 / 1035 bp (0.3 %), without gaps), and tef 1 - α (0 / 903 bp (0 %), without gaps). Morphologically, our collection is nearly identical to the holotype (ZHKU 23-0106), including the ascospores size (19–30 × 5–8 µm vs. 20–30 × 5–8 μm). Thus, we identified our strain as M. puerensis, representing a new host record on M. integrifolia. Additionally, this marks the first report of Melomastia associated with Macadamia (Table 4).
description: Published as part of Zhang, Xian, Tibpromma, Saowaluck, Karunarathna, Samantha C., Du, Tian-Ye, Han, Li-Su, Elgorban, Abdallah M., Kumla, Jaturong, Senwanna, Chanokned, Dai, Dong-Qin, Suwannarach, Nakarin & Wang, Hao-Han, 2025, Additions to the saprobic fungi (Ascomycota) associated with macadamia trees from the Greater Mekong Subregion, pp. 1-29 in MycoKeys 113 on pages 1-29, DOI: 10.3897/mycokeys.113.140031