title: ÐÐ¸Ð¾ÑÐ¾Ð²Ð¼ÐµÑÑÐ¸Ð¼ÑÐµ Ð³ÐµÐ»Ð¸ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ Ñ Ð¸ÑÐ¾Ð·Ð°Ð½Ð° Ð´Ð»Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÐºÐ»ÐµÑÐ¾Ðº
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Biocompatible gels based on chitosan for cell cultivation
Creators: name: ÐÐ°Ð»Ð¸Ðº, ÐÐ»ÐµÐ½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Ñ Ð¸ÑÐ¾Ð·Ð°Ð½Ð¾Ð²ÑÐµ Ð¼Ð°ÑÑÐ¸ÑÑ
Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð°Ð»ÑÐ½ÑÐµ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸
3Ð-Ð±Ð¸Ð¾Ð¿ÐµÑÐ°ÑÑ
ÑÐºÐ°Ð½ÐµÐ²Ð°Ñ Ð¸Ð½Ð¶ÐµÐ½ÐµÑÐ¸Ñ
Ð±Ð¸Ð¾ÑÐ¾Ð²Ð¼ÐµÑÑÐ¸Ð¼ÑÐµ Ð³ÐµÐ»Ð¸
ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÐºÐ»ÐµÑÐ¾Ðº
ÐºÐ¾Ð½Ð´ÑÐ¸ÑÑ
Ð¿Ð¾Ð»Ð¸Ð»Ð°ÐºÑÐ¸Ð´Ð½ÑÐ¹ Ð½ÐµÑÐºÐ°Ð½ÑÐ¹ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»
Ð²Ð¾ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¸Ðµ Ð¿ÐµÑÐ¸ÑÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð½ÐµÑÐ²Ð°
chitosan scaffolds
mesenchymal stem cells
3d bioprinting
tissue engineering
biocompatible gels
cell cultivation
conduits
polylactide nonwoven material
peripheral nerve repair

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description: The subject of the graduate qualification work is Â«Biocompatible gels based on chitosan for cell cultivationÂ». The work is devoted to the study of the compositions and properties of biocompatible gels based on chitosan and the possibility of cell cultivation on them. The obtained structures are supposed to be used as nerve guidance channels â conduits subsequently. Improvement of the mechanical properties of gel was achieved by adding starch. The resulting gels meet the requirements for bioinks used for 3D bioprinting. In addition, the optimal parameters for 3D bioprinting have been developed: printing temperature, print speed, travel speed and fill density. A polylactide nonwoven material was chosen as the basis for printing the conduit due to its biodegradability and low cytotoxicity. Also, the study showed high adhesion of the gels to this material. After printing, the structure remains stable over time and temperature. As a result of cytological analysis, it was concluded that the cultured mesenchymal stem cells proliferate on the gels and do not undergo necrosis the developed gels. All the above points indicate that the developed chitosan-based gels can be used as a tissue engineering preparation for the regeneration of peripheral nerves.