title: ÐÐ¸Ð½Ð°Ð¼Ð¸ÐºÐ° Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ð½ÑÑ ÑÐ¾ÐºÐ¾Ð² Ð¸ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð¾Ð², Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ ÑÐ²ÐµÑÐ¾Ð¼ Ð² Ð¿Ð¸ÑÐ°Ð¼Ð¸Ð´Ð°Ð»ÑÐ½ÑÑ Ð½ÐµÐ¹ÑÐ¾Ð½Ð°Ñ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð° Ð¼ÑÑÐ¸
title: Ð½Ð°ÑÑÐ½ÑÐ¹ Ð´Ð¾ÐºÐ»Ð°Ð´
titleType: Subtitle
title: Dynamics of light-induced membrane currents and potentials in mouse hippocampal pyramidal neurons
Creators: name: ÐÑÐ¾ÑÐµÐµÐ², ÐÐ»ÐµÐºÑÐ°Ð½Ð´Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2018
Subjects: ÐÐµÐ¹ÑÐ¾Ð½Ñ
ÐÐ¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð°Ñ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ñ
ÐÐ¸Ð¾ÑÐ¸Ð·Ð¸ÐºÐ°
Ð¿ÐµÑÐ²Ð¸ÑÐ½Ð°Ñ ÐºÑÐ»ÑÑÑÑÐ° Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð°
Ð¾Ð¿ÑÐ¾Ð³ÐµÐ½ÐµÑÐ¸ÐºÐ°
ÑÐ¾ÑÐ¾ÑÐ¾ÐºÐ¸
ÐºÐ°Ð½Ð°Ð»ÑÐ¾Ð´Ð¾Ð¿ÑÐ¸Ð½-2
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description: In the present work was investigated the dynamics of membrane currents induced by activation of channelrhodopsin-2 (ChR2) expressed in hippocampal neurons in culture. The main goal of the work was to find parameters for the light stimulus (its intensity and duration). It was shown that the dependence of the current amplitude on the light pulse duration at normal conditions is described by a right-skewed bell-shaped curve, while the dependence on the stimulus intensity is close to linear; the time duration is required to reach. Thus, was identified the optimum values ââof range for the pulse duration (10-30 ms) and the intensity (maximal values), which keep the above stability. The results of certain caveats for discovering pathologies in the activity of neurons: the case, when such dynamics loses the stability of the revealed optimal regime, it may indicate the presence of anomalous neuronal states and e.g. developing neurodegenerative diseases.