title: ÐÐ·ÑÑÐµÐ½Ð¸Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ñ ÑÐ¾Ð¼Ð°Ð½ÑÐ¸Ð½-ÑÐµÐ¼Ð¾Ð´ÐµÐ»Ð¸ÑÑÑÑÐµÐ³Ð¾ ÑÐ°ÐºÑÐ¾ÑÐ° CHD1 Ð½Ð° ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð³ÐµÐ½Ð¾Ð² ÑÐºÐ´Ð¸Ð·Ð¾Ð½Ð¾Ð²Ð¾Ð³Ð¾ ÐºÐ°ÑÐºÐ°Ð´Ð°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Study of the effect of chromatin-remodeling factor CHD1 on the expression of ecdysone cascade genes
Creators: name: ÐÐµÑÑÐ¸Ð½Ð¸Ð½Ð°, ÐÐ»ÐµÐ½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Drosophila melanogaster
CHD1
ÐÐ¦Ð Ð² ÑÐµÐ°Ð»ÑÐ½Ð¾Ð¼ Ð²ÑÐµÐ¼ÐµÐ½Ð¸
ÑÐºÐ´Ð¸Ð·Ð¾Ð½Ð¾Ð²ÑÐ¹ ÐºÐ°ÑÐºÐ°Ð´
real-time PCR
ecdisone cascade

description: Ð¦ÐµÐ»ÑÑ Ð´Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ ÑÐ°ÐºÑÐ¾ÑÐ° CHD1 Ð½Ð° ÑÑÐ¾Ð²ÐµÐ½Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² ÑÐºÐ´Ð¸Ð·Ð¾Ð½Ð¾Ð²Ð¾Ð³Ð¾ ÐºÐ°ÑÐºÐ°Ð´Ð° Drosophila melonogaster. ÐÑÐ»Ð¸ Ð¿Ð¾ÑÑÐ°Ð²Ð»ÐµÐ½Ñ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð·Ð°Ð´Ð°ÑÐ¸: 1. ÐÐ¾Ð´Ð¾Ð±ÑÐ°ÑÑ Ð¸ Ð¿ÑÐ¾Ð°Ð½Ð°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°ÑÑ Ð°ÐºÑÑÐ°Ð»ÑÐ½ÑÑ Ð½Ð°ÑÑÐ½ÑÑ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÑ Ð½Ð° ÑÐµÐ¼Ñ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ Ð¸ ÑÐ°ÐºÑÐ¾ÑÐ° CHD1. 2. ÐÑÐ´ÐµÐ»Ð¸ÑÑ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð» Ð¸Ð· Ð¶ÐµÐ»ÐµÐ· Ð¿Ð»Ð¾Ð´Ð¾Ð²ÑÑ Ð¼ÑÑÐµÐº ÑÐ»Ð¾-ÑÐ¾ÑÐ¾ÑÐ¼-ÑÐµÐ½Ð¾Ð»ÑÐ½ÑÐ¼ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼. 3. ÐÑÐ¾Ð²ÐµÑÑÐ¸ Ð°Ð½Ð°Ð»Ð¸Ð· ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð¸Ð½ÑÐµÑÐµÑÑÑÑÐ¸Ñ Ð³ÐµÐ½Ð¾Ð² Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¼Ðµ-ÑÐ¾Ð´Ð° ÐÐ¦Ð  Ð² ÑÐµÐ°Ð»ÑÐ½Ð¾Ð¼ Ð²ÑÐµÐ¼ÐµÐ½Ð¸.Â  ÐÑÐ¸ Ð½Ð°Ð¿Ð¸ÑÐ°Ð½Ð¸Ð¸ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±Ð·Ð¾ÑÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ: eLIBRARY.RU, FlyBase, Cyberleninka, PubMed, ScienceDirect, Re-searchGate. Ð¡ÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÑÑ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»Ð¸ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿Ð°ÐºÐµÑÐ° Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼ Bio-rad manager Ð¸ REST 2009. Ð ÑÐ¾Ð´Ðµ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ» Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½ Ð°Ð½Ð°Ð»Ð¸Ð· ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² ÑÐºÐ´Ð¸Ð·Ð¾Ð½Ð¾Ð²Ð¾Ð³Ð¾ ÐºÐ°Ñ-ÐºÐ°Ð´Ð° Ñ ÑÐ°Ð·Ð½ÑÑ Ð»Ð¸Ð½Ð¸Ð¹ D. melanogaster. ÐÑÐ»Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ñ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾-ÑÑÐ¸ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² E74A, E74B, Dhr3 Ð¾Ñ ÑÑÐµÑ ÑÑÐ°Ð´Ð¸Ð¹ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ñ Ð´ÑÐ¾Ð·Ð¾ÑÐ¸Ð»Ñ Ð´Ð»Ñ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ Ð»Ð¸Ð½Ð¸Ð¹ Ð¼ÑÑÐµÐº Ð¸ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÐµÐ½Ñ Ð² Ð²Ð¸Ð´Ðµ Ð³ÑÐ°ÑÐ¸ÐºÐ¾Ð². ÐÐ»Ñ Hr4 Ð¸ Chd1 Ð¿Ð¾Ð»ÑÑÐ¸ÑÑ Ð´Ð¾ÑÑÐ¾Ð²ÐµÑÐ½ÑÐµ Ð³ÑÐ°ÑÐ¸ÐºÐ¸ Ð½Ðµ ÑÐ´Ð°Ð»Ð¾ÑÑ, Ð½ÐµÑÐ¼Ð¾ÑÑÑ Ð½Ð° Ð¼Ð½Ð¾Ð³Ð¾ÐºÑÐ°ÑÐ½ÑÐµ Ð¿Ð¾Ð²ÑÐ¾ÑÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°. ÐÐ»Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÑÐ½ÐºÑÐ¸Ð¹ ÑÐ°ÐºÑÐ¾ÑÐ° CHD1 Ð¼Ð¾Ð¶Ð½Ð¾ ÑÑÐ¿ÐµÑÐ½Ð¾ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°ÑÑ Ð¼Ð¾Ð´ÐµÐ»Ñ, Ð¾ÑÐ½Ð¾Ð²Ð°Ð½Ð½ÑÑ Ð½Ð° ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ ÐºÐ°ÑÐ°Ð»Ð¸ÑÐ¸ÑÐµÑÐºÐ¸ Ð½Ðµ Ð°ÐºÑÐ¸Ð²Ð½Ð¾Ð¹ ÑÐ¾ÑÐ¼Ñ ÑÑÐ¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ°, Ð»Ð¸Ð±Ð¾ CHD1 Ð´Ð¸ÐºÐ¾Ð³Ð¾ ÑÐ¸Ð¿Ð° Ð² ÐºÐ»ÐµÑÐºÐ°Ñ ÑÐ»ÑÐ½Ð½ÑÑ Ð¶ÐµÐ»ÐµÐ·. Ð ÑÐ¾Ð´Ðµ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° ÑÐ°ÐºÐ¶Ðµ Ð±ÑÐ» Ð´Ð¾ÑÑÐ¾Ð²ÐµÑÐ½Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½ Ð¿Ð¾Ð½Ð¸Ð¶ÐµÐ½Ð½ÑÐ¹ ÑÑÐ¾Ð²ÐµÐ½Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð° E74A Ñ Ð½ÑÐ»Ñ-Ð¼ÑÑÐ°Ð½ÑÐ° Ð¿Ð¾ Ð³ÐµÐ½Ñ Chd1, Ð° ÑÐ°ÐºÐ¶Ðµ, Ð¾ÑÐ»Ð¸ÑÐ°ÑÑÐ°ÑÑÑ Ð¾Ñ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½Ð¾Ð¹ Ð»Ð¸Ð½Ð¸Ð¸, Ð½Ð¾ ÑÑÐ¾Ð¶Ð°Ñ Ð¼ÐµÐ¶Ð´Ñ ÑÐ¾Ð±Ð¾Ð¹ Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐ° ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð° E74AÂ  Ð¼ÑÑÐ°Ð½ÑÐ½ÑÑ Ð»Ð¸Ð½Ð¸Ð¹ Ð»Ð¸Ð½Ð¸Ð¸ ÑÐ¾ ÑÐ²ÐµÑÑÐ¿ÑÐ¾Ð´ÑÐºÑÐ¸ÐµÐ¹ Ð½Ð°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ Ð¸ Ð½Ðµ Ð°ÐºÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° CHD1.Â  ÐÐ°Ð½Ð½Ð¾Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð±ÑÐ»Ð¾ ÑÐ´ÐµÐ»Ð°Ð½Ð¾ Ð½Ð° Ð±Ð°Ð·Ðµ ÐÐ°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½-ÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð³ÐµÐ½ÐµÑÐ¸ÐºÐ¸ ÐÐÐ Ð Â«ÐÐÐ¦ ÐÑÑÑÐ°ÑÐ¾Ð²ÑÐºÐ¸Ð¹ Ð¸Ð½ÑÑÐ¸ÑÑÑÂ» â ÐÐÐ¯Ð¤.Â 
description: The topic of the final qualifying work: Â«Study of the effect of chromatin-remodeling factor CHD1 on the expression of ecdysone cascade genesÂ». The aim of this study was the effect of the CHD1 factor on the level of gene expression of the ecdysone cascade of Drosophila melonogaster. The following tasks were set: 1. To select and analyze the current scientific literature on the topic of genet-ic research and the CHD1 factor. 2. To isolate the genetic material from the glands of fruit flies by the chloro-form-phenolic method. 3. To analyze the expression of the genes of interest using a real-time PCR method. When writing the literary review, the following databases were used: eLi-brary.RU, FlyBase, Cyberleninka, PubMed, ScienceDirect, ResearchGate. Statistical processing of experimental data was carried out using the Bio-rad manager and REST 2009 software package. In the course of the work, the expression of ecdysone cascade genes in different D. melanogaster lines was analyzed. The results of the dependence of the expres-sion of the E74A, E74B, and Dhr3 genes on the three stages of drosophila devel-opment, as well as various fly lines, were obtained were obtained and presented in the form of graphs. It was not possible to obtain reliable graphs for Hr4 and Chd1, despite repeated repetitions of the experiment. To study the functions of the CHD1 factor, a model based on the expression of a catalytically inactive form of this protein or wild-type CHD1 in salivary gland cells can be used. The influ-ence of the CHD1 factor on the development of D. Melanogaster in the model system was studied. The analysis also reliably showed a reduced level of expression of the E74A gene in the null mutant for the Cdh1 gene, as well as the dynamics of expression of the E74A gene, which differs from the control line, but is similar to each other mutant lines are lines with overproduction of native and inactive CSD1 protein. This study was done on the basis of the Laboratory of experimental genetics of OMRB Â«SIC Kurchatov InstituteÂ» â PIAF.