Microseira wollei G. B. McGregor and Sendall ex Kennis 2017
Creators: name: Bae, Eun Hee
name: Kang, Jae-Shin
name: Park, Chong-Sung
Publisher: name: Zenodo
Year: 2020
Subjects: Biodiversity
Taxonomy
Bacteria
Cyanobacteria
Cyanobacteriia
Cyanobacteriales
Aerosakkonemataceae
Microseira
Microseira wollei

description: Microseira wollei (Farlow ex Gomont) G.B.McGregor and Sendall ex Kennis 2017
Basionym: Plectonema wollei Farlow ex Gomont 1892 Homotypic synonym: Lyngbya wollei (Farlow ex Gomont) Speziale and Dyck 1992
Type specimen: 945406 (syntype, NY)
Habitat: The filaments of Microseira wollei were found in sublittoral habitat of a freshwater river entwined in the basal part of aquatic macrophytes or plants. Collection site: The riverside of 7 km upper stream from Gangjeong-Goryeong weir at Dalsung-gun, Daegu-city, Nakdong River (35°51 ʹ 31.6 ʺ N 128°23 ʹ 23.0 ʺ E).
Specimens: NIBRCY0000001021 (collected by E.H. Bae, no. EHB20200513-1, 35°51 ʹ 31.6 ʺ N 128°23 ʹ 23.0 ʺ E, 13 May 2020), NIBRCY0000001022 (collected by E.H. Bae, no. EHB20200513-2, 35°51 ʹ 31.6 ʺ N 128°23 ʹ 23.0 ʺ E, 13 May 2020), NIBRCY0000001023 (collected by E.H. Bae, no. EHB20200513-3, 35°51 ʹ 31.6 ʺ N 128°23 ʹ 23.0 ʺ E, 13 May 2020), NIBRCY0000001024 (collected by E.H. Bae, no. EHB20200616-1, 35°51 ʹ 31.6 ʺ N 128°23 ʹ 23.0 ʺ E, 16 June 2020) in KB.
Habit: Microseira wollei was identified as remarkably large-celled, greenish black filamentous, mat-forming characteristics, observed with naked eyes.
Morphology
Thallus a prostrate, greenish blue-black tough mat of densely entangled filaments (Fig. 1). Filaments straight to variously contorted, and infrequently false-branched. An individual filament consists of an uniseriate file of discoids to cylindrical cells. Cell division occurs throughout the length of the filament and apex is flat at immediately after trichome division. Sheath colorless, hyaline, when old distinctly thickened, roughened and colored, open at the apex. Trichomes isopolar, uniformly cylindrical, up to 70 μm wide; not or slightly constricted at the cross-walls. Cells discoid, coffee bean shaped in lateral view; contents finely granular, without aerotopes; apical cells rounded, without a calyptra and thickened outer cell wall (Fig. 2).
Ultrastructures by transmission electron microscopy
(TEM)
Filaments of M. wollei were cross-sectioned to observe their ultrastructural patterns of thylakoids and intracellular contents by TEM. The trichome filaments were covered by cell wall and outer thick sheath (Fig. 3A). The cytoplasm was filled with numerous thylakoids (Fig. 3B). Membranous thylakoids were extended from near cell membrane and densely stacked as bundle (Fig. 3C, D). The bundles of thylakoids were located irregularly, not parietally nor radially.
Molecular phylogeny
A total of 1,267 bp of the 16S rRNA gene and 318 bp nifH (nitrogenase iron protein) gene sequences of were analyzed. All sequences of eight isolates from Korea were identical based on these two genes. Among 1,267 bp the 16S rRNA gene sequences, sequences of the Korean population were different from 0.02% (2 bp) to the Japanese population (NIES-4236), 1.2 - 1.3% to the Australian population, and 2.5 - 3.7% to USA population and other geographical populations in NCBI database. While, nucleotides of nifH gene showed distance as 8.4 - 8.7% to Australian samples and 3.5 - 3.8% to other populations. The neighbor joining trees are presented for 16S rRNA gene (Fig. 4) and nifH gene (Fig. 5). All trees supported our conspecific speculation of the Korean population of M. wollei.
Cyanotoxin analysis by PCR of cyr genes: The cyrF gene of 447 bp and cyrJ gene of 626 - 657 bp were not positive in all Korean samples.
description: Published as part of Bae, Eun Hee, Kang, Jae-Shin & Park, Chong-Sung, 2020, New report on cyanophyte in Korea, Microseira wollei (Farlow ex Gomont) G. B. McGregor and Sendall ex Kennis (Oscillatoriaceae), pp. 210-217 in Journal of Species Research 9 (3) on pages 213-215, DOI: 10.12651/JSR.2020.9.3.210, http://zenodo.org/record/13140633