title: ÐÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ Ð¼ÐµÑÐ¾Ð´Ð° Ð±ÐµÑÐºÐ°Ð¿ÐµÐ»ÑÐ½Ð¾Ð³Ð¾ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ°ÑÐ¿ÑÐ»ÐµÐ½Ð¸Ñ Ð´Ð»Ñ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Ð¼Ð¸ÑÐµÐ½Ð¸ Ð´Ð»Ñ ÐÐÐÐÐ-Ð¼Ð°ÑÑ-ÑÐ¿ÐµÐºÑÑÐ¾Ð¼ÐµÑÑÐ¸Ð¸ Ð¾ÐºÑÐ¸Ð´Ð°Ð¼Ð¸ Ð¿ÐµÑÐµÑ Ð¾Ð´Ð½ÑÑ Ð¼ÐµÑÐ°Ð»Ð»Ð¾Ð²
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Application of the drop-free electric spraying method for MALDI mass spectrometry target modification by transition metal oxides
Creators: name: Ð¡ÐµÑÐ³Ð¸ÐµÐ½ÐºÐ¾, ÐÐ½Ð°ÑÑÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: Ð±ÐµÑÐºÐ°Ð¿ÐµÐ»ÑÐ½Ð¾Ðµ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ°ÑÐ¿ÑÐ»ÐµÐ½Ð¸Ðµ
ÐÐÐÐÐ-Ð¼Ð°ÑÑ-ÑÐ¿ÐµÐºÑÑÐ¾Ð¼ÐµÑÑÐ¸Ñ
Ð¿ÑÐ¾Ð±Ð¾Ð¿Ð¾Ð´Ð³Ð¾ÑÐ¾Ð²ÐºÐ°
ÑÐ¾ÑÐ±ÑÐ¸Ñ
Ð¾ÐºÑÐ¸Ð´Ñ Ð¿ÐµÑÐµÑ Ð¾Ð´Ð½ÑÑ Ð¼ÐµÑÐ°Ð»Ð»Ð¾Ð²
Ð¼ÐµÑÐ°Ð»Ð»-Ð°ÑÑÐ¸Ð½Ð½ÑÐµ ÑÐ¾ÑÐ±ÐµÐ½ÑÑ
drop-free electric spraying
MALDI mass spectrometry
sample preparation
sorption
transition metal oxides
metall-affinity sorbents

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description: This work is devoted to the development of a method allowing sample preparation on the target surface for MALDI mass spectrometry. During the drop-free electric spraying of suspensions containing copper, nickel and cobalt oxides, the target surface for MALDI was modified at atmospheric pressure under normal conditions. Tasks that were solved in the course of work: 1) Optimization of the installation layout for drop-free electrospray and selection of optimal conditions for electrospray of suspensions. 2) Modification of the MALDI target by electrospray with copper, nickel and cobalt oxides. 3) Carrying out metal-affinity extraction of chlorine-containing human hemoglobin adducts on the obtained spots of metal-affinity sorbents on the target. Modification of the MALDI target by electrospray with subsequent affinity en-richment was carried out on the basis of the Institute of Analytical Instrumentation of the Russian Academy of Sciences. MALDI mass spectrometric analysis was carried out in the resource center of the Science Park of St. Petersburg State University "Development of molecular and cellular technologies." As a result, sorbent spots were obtained on the target surface, sample preparation of hydrolyzate samples containing adducts of a chloroacetanilide derivative was carried out on these spots. After metal affinity extraction, adduct signals were detected and identified in the hydrolyzate samples, with the ratio of the modified peptide to unmodified 1: 100. This method of sample preparation allows to increase the sensitivity of mass spectrometric analysis.