title: ÐÐ°ÑÑÑÐµÐ½Ð¸Ðµ ÑÑÑÑÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÐ¸ÐºÐ»Ð° Ñ ÑÐ°Ð¼Ð¾Ðº ÐºÑÑÑ Ð¿Ð¾ÑÐ»Ðµ Ð²Ð¸ÑÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÑÑÐµÑÑÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Estrous cycle disorder in female rats after vital stress
Creators: name: ÐÐµÐ¼ÑÐµÐ²Ð°, ÐÐ¾Ð»Ð¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Ð²Ð¸ÑÐ°Ð»ÑÐ½ÑÐ¹ ÑÑÑÐµÑÑ
Ð¿Ð¾Ð»Ð½Ð¾Ð³ÐµÐ½Ð¾Ð¼Ð½Ð¾Ðµ Ð¼ÐµÑÐ¸Ð»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÐÐÐ
ÑÐµÐºÑÐ°Ð»ÑÐ½Ð°Ñ ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÐ°
ÑÐµÐ¿ÑÐ¾Ð´ÑÐºÑÐ¸Ð²Ð½Ð°Ñ ÑÐ¸ÑÑÐµÐ¼Ð°
ÑÑÑÑÐ°Ð»ÑÐ½ÑÐ¹ ÑÐ¸ÐºÐ»
vital stress
genome-wide DNA methylation
rectal temperature
reproductive system
estrous cycle

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description: The subject of the graduate qualification work is âestrous cycle disorder in female rats after vital stressâ. The work considers the delayed effect of vital stress on the weight of animals and rectal temperature at different stages of the oestrous cycle. One of the possible epigenetic mechanisms of regulation of gene expression in the formation of pathologies of the reproductive system in neuropsychiatric disorders was investigated â DNA methylation.Modelling of vital stress in this work was carried out to the rats experiencing a threat to their own lives â observing the death of a relative from the actions of a predator â boa constrictor. Assessment of the level of genome-wide DNA methylation by CCGG sites was carried out by enzymatic hydrolysis of genomic DNA by MspI and HpaII restrictases. The results indicate changes in rectal temperature in the proestrus and diestrus, the duration of the reproductive cycle and its stages, as well as the absence of weight gain in animals subjected to stress. There was a decrease in genome-wide DNA methylation in bone marrow and leukocytes by peripheral blood. In addition, the degree of influence of vital stress on the epigenome of the cells of the hematopoietic system differs depending on the phase of oestrous cycle, which may be explained by the different hormonal status of individuals at these stages. The results determine the disorders of the reproductive system of female rats, manifested, in particular, in the development of anovulatory syndrome and violation of the process of ovogenesis. Thus, the results obtained may be used to understand the pathogenesis of disorders of the reproductive system caused by psychogenic trauma and to develop ways to correct them.