title: ÐÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð½ÑÐºÐ»ÐµÐ¸Ð½Ð¾Ð²ÑÑ ÐºÐ¸ÑÐ»Ð¾Ñ Ð² Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð¹ Ð¿ÑÐ¾ÑÐ¾ÑÐ½Ð¾Ð¹ ÐºÐ°Ð¼ÐµÑÐµ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Isolation of nucleic acids in a modified flow chamber
Creators: name: Ð¡Ð¸Ð¼Ð¾Ð½Ð¾Ð²Ð°, ÐÑÐµÐ½Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð½ÑÐºÐ»ÐµÐ¸Ð½Ð¾Ð²ÑÐµ ÐºÐ¸ÑÐ»Ð¾ÑÑ
Ð¿ÑÐ¾ÑÐ¾ÑÐ½Ð¾Ðµ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
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Ð²ÑÑÐ¾ÐºÐ¾ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾Ðµ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ
nucleic acids
flow concentration
isolation
polymerase chain reaction
sample preparation
high-performance isolation

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description: The object of the study is experimental cells for the isolation of nucleic acids using the flow method. The purpose of the work is to study the parameters and efficiency of nucleic acid isolation in modified flow cells. As a result of the research, cells were designed and created for the isolation of nucleic acids using the flow method; The results obtained suggest that this method of nucleic acid isolation is suitable for sample preparation of genetic material for analysis by RT-PCR. Technologies based on this approach can be used to create isolation systems, create devices for isolating and concentrating NAs, and developing methods for flow-through isolation of nucleic acids. The highest efficiency of nucleic acid extraction can be achieved by passing the sample three times through a flow cell with one-way modification and a channel height of 5 ml/min. To determine the efficiency of isolation, the results of RT-PCR analysis of isolated samples were compared with isolation performed âmanuallyâÂ  ccording to the method recommended by the manufacturer of the reagent kit. The result of the experimental study is reproducible, which indicates the correctness of the implementation of the methodology.