Additional file 1 of Engineering sigma factors and chaperones for enhanced heterologous lipoxygenase production in Escherichia coli
Creators: name: Pang, Cuiping
name: Zhang, Guoqiang
name: Liu, Song
name: Zhou, Jingwen
name: Li, Jianghua
name: Du, Guocheng
Publisher: name: figshare
Year: 2022
Subjects: subject: Genetics
subject: FOS: Biological sciences

description: Additional file 1: Fig S1. Schematic diagram of spontaneous assembly fluorescent plasmid. Fig S2. Establishment of the relationship between LOX activity, fluorescence intensity, LOX soluble level. A. Correlation between fluorescence intensity and protein concentration; B. Correlation between fluorescence intensity and LOX activity. Fig S3. Clusters of Orthologous Groups of proteins (COG) A and Kyoto Encyclopedia of Genes and Genomes (KEGG) B analysis of common differential gene A Three-way Venn diagram showing the number of genes differentially expressed (fold change 3, adjusted pvalue≤0.05) between E. coli expressing LOX, E. coli co-expressing LOX with the wild-type Skp and E. coli co-expressing LOX with mutants of Skp and RpoH. B Genes differently expressed analysis statistical histogram. The horizontal axis is the name of the difference comparison, and the vertical axis is the number of up-down difference genes. Fig S4. Significant enrichment scatter of Orthologous Groups of proteins (COG) analysis of common differential gene. A Significant enrichment COG scatter diagram of differentially downregulated genes between S and WT. B Significant enrichment COG scatter diagram of differentially upregulated genes between S and WT. C Significant enrichment COG scatter diagram of differentially downregulated genes between Sopt and WT. D Significant enrichment COG scatter diagram of differentially upregulated genes between Sopt and WT. E Significant enrichment COG scatter diagram of differentially downregulated genes between Ropt and WT. F Significant enrichment COG scatter diagram of differentially upregulated genes between S and WT. The vertical axis represents the function annotation information, and the horizontal axis represents the rich factor corresponding to the function. The size of Qvalue is represented by the color of the dot. The smaller the Qvalue, the closer the color is to red. The dot size represents the number of differential genes contained in each function. Fig. S5 Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of common differential gene. A–C Scatter diagram of significant enrichment function of differently expressed genes in the metabolic pathways between Sopt and WT, Sopt and S, Ropt and WT, respectively. The vertical axis represents the function annotation information, and the horizontal axis represents the rich factor corresponding to the function. The size of Qvalue is represented by the color of the dot. The smaller the qvalue, the closer the color is to red. The dot size represents the number of differential genes contained in each function. D Heatmap showing differentially expressed genes (fold change 3, adjusted p-value≤0.05) between E. coli expressing LOX, E. coli co-expressing LOX with the wild-type skp and E. coli co-expressing LOX with mutants of Skp. Rows (genes) were clustered hierarchically. Table S1. Plasmids and strains used in this work. Table S2. Oligonucleotides used in this study. Table S3. Mutant residue analysis of GroES, Skp and RpoH directed evolution Analysis of LOX expression level by Image Lab.