Additional file 1 of Laccase-mimicking Mn–Cu hybrid nanoflowers for paper-based visual detection of phenolic neurotransmitters and rapid degradation of dyes
Creators: name: Le, Thao Nguyen
name: Le, Xuan Ai
name: Tran, Tai Duc
name: Lee, Kang Jin
name: Kim, Moon Il
Publisher: name: figshare
Year: 2022
Subjects: subject: Space Science
subject: Medicine
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: FOS: Biological sciences
subject: 69999 Biological Sciences not elsewhere classified

description: Additional file 1: Fig. S1. SEM images of H–Mn–Cu NFs after incubation for (a) 0, (b) 12, (c) 24, (d) 48, and (e) 72 h. Insets represent high-resolution SEM images. Fig. S2. Elemental mapping of H–Mn–Cu NFs. Fig. S3. (a) XRD pattern and (b) FT-IR spectra of MnO2 NFs, amine-functionalized (APTES)-MnO2 NFs, Cu3(PO4)2 precipitates, and H–Mn–Cu NFs. (c) BET surface areas of MnO2 NFs, APTES-MnO2 NFs, and H–Mn–Cu NFs. Fig. S4. XPS full scan spectrum of H-Mn-Cu NFs. Fig. S5. High-resolution XPS spectra of Cu for (a) H-Mn-Cu NFs and (b) Cu3(PO4)2 precipitates, and Mn for (c) H-Mn-Cu NFs and (d) bare MnO2 NFs. Fig. S6. Effects of (a) pH and (b) temperature on laccase-like activity of H–Mn–Cu NFs. Fig. S7. Comparison of stabilities between H–Mn–Cu NFs and free laccase in the following ranges: (a) pH, (b) temperature, (c) ionic concentration (NaCl), and (d) incubation period under shaking conditions. Fig. S8. Steady-state kinetic assays of (a) H–Mn–Cu NFs and (c) free laccase for epinephrine and their corresponding Lineweaver–Burk plots (b and d). Fig. S9. Comparison of laccase-like dopamine-detecting activity of H-Mn-Cu NFs with those of control samples. Fig. S10. Dose–response curves and corresponding linear calibration plots for determining (a, b) dopamine and (c, d) epinephrine using a well plate-based assay with free laccase. Fig. S11. MALDI-TOF mass spectrometry for H-Mn-Cu NFs-mediated degradation of (a) CV, (b) NR, and (c) RB at different incubation time of 0 h, 24 h, and 48 h. Fig. S12. Comparison of color signals of the H–Mn–Cu NF-incorporated paper microfluidic device for detecting both dopamine and epinephrine with those of controls [MnO2 NFs, Cu3(PO4)2 precipitates, and free laccase]. Fig. S13. Optimization of the wax barrier thickness of the paper microfluidic device to prevent flow leakage. (a) Paper devices after printing, (b) paper devices after wax melting, and (c) paper devices after adding a colored reagent (rhodamine B dye). The barrier thicknesses from top-left to down-right were 0.05, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, and 0.9 mm. Among these, 0.7 mm was used in this study. Fig. S14. Optimization of the reaction time for dopamine and epinephrine detection by H–Mn–Cu NF-incorporated paper microfluidic devices. Paper devices (a) before adding 4-AP, (b) after adding 4-AP, and for detecting dopamine and epinephrine (20 µM) during the reaction for (c) 0 min, (d) 2 min, (e) 5 min, (f) 10 min, (g) 15 min, (h) 20 min, (i) 25 min, and (j) 30 min. Among these, a 10 min reaction was used in this study. Fig. S15. Optimization of the employed concentrations of (a) 4-AP and (b) H–Mn–Cu NFs to prepare the H–Mn–Cu NF-incorporated paper microfluidic device for dopamine detection (20 µM). Consequently, 5 mg mL-1 4-AP and 10 mg mL-1 H–Mn–Cu NFs were used in this study. Fig. S16 Long-term storage stability of the H–Mn–Cu NF-incorporated paper microfluidic device at RT. Table S1. Comparison of the linear range and LOD values of H-Mn-Cu NFs with those of recent colorimetric assays for dopamine and epinephrine. Table S2. Detection precision of the H–Mn–Cu NF-based paper microfluidic device for the determination of dopamine and epinephrine levels in spiked human serum samples.