Additional file 1 of LncRNA HOXD-AS1 promotes the metastasis of human hepatocellular carcinoma via modulating miR-326/SLC27A4
Creators: name: Ji, Wenbin
name: Wang, Qunfeng
name: Yang, Jian
Publisher: name: figshare
Year: 2020
Subjects: subject: Biochemistry
subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Chemical Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Cancer

description: Additional file 1: Figure S1. The overall survival of HCC patients between the low and high HOXD-AS1 expression group was compared. Figure S2.A. HepG2 and SMMC-7721 cell was transfected with HOXD-AS1 siRNA and the level of HOXD-AS1 was determined by qPCR. **P&lt;0.01 compared to control. B. The level of HOXD-AS1 in tumor tissues formed by sh-HOXD-AS1 or sh-NC transfected cells was analyzed using qPCR. **P&lt;0.01 compared to sh-NC. Figure S3. The effects of HOXD-AS1 on HCC cell growth and colony formation. A. The level of HOXD-AS1 was determined by qPCR in BEL7402 and HuH7 cells after transfection with HOXD-AS1 or control vector. B. CCK-8 assay showed that the proliferation of BEL7402 and HuH7 was stimulated by HOXD-AS1. C. The colony formation abilities in BEL7402 and HuH7 cells were stimulated by HOXD-AS1. *P&lt;0.01 compared to control. Figure S4. The effects of HOXD-AS1 on HCC cell growth, migration and invasion. A. The level of HOXD-AS1 was determined by qPCR in BEL7402 and HuH7 cells after transfection with HOXD-AS1 siRNA. B. CCK-8 assay was used to analysis the viability in HOXD-AS1 siRNA or scramble transfected HCC cell. C. Colony formation assay using HOXD-AS1 siRNA or scramble transfected HCC cell. D. The migration of BEL7402 and HuH7 cells after transfection of HOXD-AS1 siRNA was detected using wound healing assay. E. The invasion abilities of HepG2 and SMMC-7721 cells after transfection of HOXD-AS1 siRNA were detected by transwell assay. *P&lt;0.01 compared to control. Figure S5. A. The potential targets of HOXD-AS1 were identified using bioinformatics analysis tool, starbase v2.0 (http://starbase.sysu.edu.cn/mirLncRNA.php). B. The miRNAs that were downregulated in response to HOXD-AS1 overexpression in both HepG2 and SMMC-7721 cells. Figure S6. A. The levels of miR-326 were determined by qPCR in HepG2 and SMMC-7721 cells after transfection with miR-326 mimics, miR-326 inhibitor or control miRNA. B. HepG2 cells were transfected with HOXD-AS1 siRNA, miR-326 inhibitor or both and the level of miR-326 was detected using qPCR assay. **P&lt;0.01 compared to control, ##P&lt;0.01 compared to miR-326 inhibitor. Figure S7.The promoted effect of HOXD-AS1 on colony formation and invasion could be reversed by miR-326 in HepG2 and SMMC-7721 cells. A. The level of HOXD-AS1 in HepG2 cells was measured by qPCR assay after transfected with HOXD-AS1, miR-326 or both. B. The growth of HepG2 cell was measured by colony formation assays after transfected with HOXD-AS1, miR-326 or both. C. The invasion abilities of HepG2 cell after transfected with HOXD-AS1, miR-326 or both. **P&lt;0.01 compared to control, ##P&lt;0.01 compared to miR-326. Figure S8. The association between SLC27A4 and miR-326 in HCC tissues was evaluated by qPCR assay. Table S1. Association of lncRNA HOXD-AS1 expression with clinicopathologic features in patients with HCC. Table 2. Association of miR-326 expression with clinicopathologic features in patients with HCC.