title: ÐÑÐ»ÑÑÐ¸Ð¿Ð»ÐµÐºÑÐ½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð· ÑÐ°ÐºÑÐ¾ÑÐ¾Ð² Ð²Ð¾ÑÐ¿Ð°Ð»ÐµÐ½Ð¸Ñ Ð² ÑÑÐ²Ð¾ÑÐ¾ÑÐºÐµ ÐºÑÐ¾Ð²Ð¸ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð½Ð¾Ð²ÑÑ Ð¼Ð°ÑÑ-ÑÐ¿ÐµÐºÑÑÐ¾Ð¼ÐµÑÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð¿Ð¾Ð´Ñ Ð¾Ð´Ð¾Ð²
title: Ð½Ð°ÑÑÐ½ÑÐ¹ Ð´Ð¾ÐºÐ»Ð°Ð´
titleType: Subtitle
title: Multiplex analysis of inflammation factors in serum using the MALDI-TOF mass spectrometry method
Creators: name: Ð¢Ð°ÑÐ°ÑÐºÐ¸Ð½, ÐÐ»ÐµÐºÑÐ°Ð½Ð´Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: ÐÐ°ÑÑ-ÑÐ¿ÐµÐºÑÑÐ¾Ð¼ÐµÑÑÐ¸Ñ
Ð°Ð»ÑÑÐ°-2-Ð¼Ð°ÐºÑÐ¾Ð³Ð»Ð¾Ð±ÑÐ»Ð¸Ð½
ÑÐµÑÑÐ¸Ð½ Ð
ÑÑÐ²Ð¾ÑÐ¾ÑÐ¾ÑÐ½ÑÐ¹ Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´ Ð
ÑÐ¸Ð½Ð´ÑÐ¾Ð¼ ÑÐ¸ÑÑÐµÐ¼Ð½Ð¾Ð¹ Ð²Ð¾ÑÐ¿Ð°Ð»Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÐµÐ°ÐºÑÐ¸Ð¸
ÑÐµÐ¿ÑÐ¸Ñ
alpha-2-macroglobulin
fetuin A
serum amyloid A
systemic inflammatory response syndrome
sepsis

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description: A novel multiplex analysis based on Matrix-assisted Laser Desorption/Ionization Time-Of-Flight (MALDI-TOF) MS for the targeted quantification of the following inflammatory markers: alpha-2-macroglobulin (Î±2-MG), alpha-2-HS-glycoprotein (fetuin A) and serum amyloid A (SAA) in human blood serum for the diagnosis of systemic inflammatory response syndrome (SIRS) and sepsis using isotopomers, peptides that contain stable isotopes of oxygen-18(18O) has been established. The proposed approach shows the possibility of using the structural and functional properties of the analyzed compounds in determining their concentration, which eliminates the need for preliminary fractionation of samples. The proposed method for quantitative assessment of these 3 biomarkers allows achieving results within a couple of hours, which is a significant advantage in comparison to standard diagnostic methods. For 87 serum specimens, obtained from patients with pyo-inflammatory soft tissue diseases, influenza A virus and coronavirus, MALDI-TOF MS and enzyme-linked immunosorbent assay (ELISA) were used to determine the levels of the following inflammatory markers: Î±2-MG, fetuin A and SAA. The levels of fetuin A and SAA in serum were significantly different in hospital patients compared with the control group. We were also able to distinguish groups with pyo-inflammatory soft tissue diseases, viral infection and control group using the combination of fetuin A and SAA levels.