MOESM1 of Identification of developmental stage and anatomical fraction contributions to cell wall recalcitrance in switchgrass
Creators: name: Crowe, Jacob
name: Feringa, Nicholas
name: Pattathil, Sivakumar
name: Merritt, Brian
name: Foster, Cliff
name: Dines, Dayna
name: Ong, Rebecca
name: Hodge, David
Publisher: name: Figshare
Year: 2017
Subjects: subject: Biophysics
subject: Biochemistry
subject: Space Science
subject: Microbiology
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: FOS: Chemical sciences
subject: Ecology
subject: Immunology
subject: FOS: Clinical medicine
subject: FOS: Clinical medicine
subject: Developmental Biology
subject: Plant Biology

description: Additional file 1: Table S1. Compiled AIR + destarched cell wall polysaccharides, acetate, and lignin content for five internodes of three switchgrass anatomical fractions. Samples are presented as an average (n = 3) with standard deviations following in additional Table S2. Table S2. Standard deviations for compiled AIR + destarched cell wall polysaccharides, acetate, and lignin content for five internodes of three switchgrass anatomical fractions. Samples are presented as an average (n = 3) with standard deviations following. Figure S1. Cell wall autofluorescence of switchgrass organ cross-sections from internode I2. Confocal laser scanning microscopy carried out using laser excitation wavelengths of 405 nm and 543 nm. Tissue types are denoted from the following notation: Ep – epidermis, Fb – fiber bundles, Vb – vascular bundles, Ph – phloem, X – xylem, Pc – Parenchyma cells. Scale bars represent 200 µm. Figure S2. Heat map abundance of major non-cellulosic glycan epitopes in oxalate, carbonate, 1 M KOH, 4 M KOH, chlorite, and 4 M KOH PC extracts from cell walls of stem, sheath, and leaf samples of internodes I1. Extracts were screened by ELISA using a comprehensive suite of cell wall glycan-directed mAbs as described in materials and methods. Binding response values are depicted as heat maps with black-red-bright yellow color scheme representing from no binding to strongest binding. The amount of carbohydrate material recovered per gram of AIR is depicted in the bar graphs (purple) above the heat maps. The panel on the right-hand side of the heat map shows the groups of mAbs based on the class of cell wall glycan they each recognize. Figure S3. Heat map abundance of major non-cellulosic glycan epitopes in oxalate, carbonate, 1 M KOH, 4 M KOH, chlorite, and 4 M KOHPC extracts from stem internodes I1-I5. Extracts were screened by ELISA using a comprehensive suite of cell wall glycan-directed mAbs as described in materials and methods. Binding response values are depicted as heat maps with black-red-bright yellow color scheme representing from no binding to strongest binding. The amount of carbohydrate material recovered per gram of AIR is depicted in the bar graphs (purple) above the heat maps. The panel on the right-hand side of the heat map shows the groups of mAbs based on the class of cell wall glycan they each recognize.