Clevelandella undefined-4
Creators: name: Kotyk, Michael
name: Bourland, William A.
name: Soviš, Matyáš
name: Méndez-Sánchez, Daniel
name: Škaloud, Pavel
name: Varadínová, Zuzana Kotyková
name: Čepička, Ivan
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Chromista
Ciliophora
Heterotrichea
Heterotrichida
Clevelandellidae
Clevelandella
Clevelandella undefined-4

description: Clevelandella sp. 4
Clevelandella sp. 4 was detected in both dissected STR hosts in which it was highly abundant.
Description based on STR population: Large-sized Clevelandella (in vivo on average 152 v 20 × 63 v 10 µm, range 127–185 µm, N = 9), spade-shaped, and dorsoventrally flattened. Left cell margin conspicuously notched at the base of peristomial projection, the lobe does not overhang the peristomial projection. The peristomial projection constituted approx. one-third to onequarter of cell length.
showing sutural kinetofragments (white arrow). E, left lateral view showing ciliated cytoproct (black arrow), unciliated parts of the dorsal (black asterisk) and ventral (white asterisk) somatic kineties, the peristomial overture (white arrow) and cilia of the circumperistomial kineties (white arrowhead). F, right lateral view showing the right suture (black arrow) between dorsal (black asterisk) and ventral (white asterisk) somatic kineties and sutural kinetofragments (white arrow, compare Fig. 23P), the margin of the peristomial overture (white arrowhead), and the cilia of the adoral membranelles (black arrowhead). Scale bars: 20 µm (A, B, E, F), 5 µm (C, D).
Morphological analyses of cells
A NMDS (non-parametric multi-dimensional scaling) analysis of 455 morphologically characterized protargol-stained cells showed a significant grouping of cells based on their affiliation to particular species (Fig. 35). Two clusters were clearly separated along the first NMDS axis, based on the presence/absence of the peristomial projection. The first cluster consisted of Anteclevelandella constricta, C. klobasa, P. brevis in ‘brevis’ form and P. brevis in ‘simplex’ form; the second cluster contained remaining species. The second NMDS axis was related to peristomial projection length, macronucleus length, and body width (variable scores -0.054, 0.047, and 0.040, respectively). The linear discriminant analysis (LDA) resulted in the model accuracy of 0.892, meaning that 89.2% of the cells were correctly assigned to the appropriate species based on the morphological traits only. The mean accuracy for all the species was 85%. According to the classification matrix (Supporting Information, Table S49), the lowest correct discrimination levels were recovered in Rhynchoclevelandella sp. 2 (14.3%) and C. kidderi (55.6%).
The mean shrinking during protargol preparations for all Clevelandellidae species combined is 18.6 v 7.4% for length and 16.6 v 10.9% for width. However, close observation on shrinking of individual species (Supporting Information, Table S50) uncovered the striking difference among species, with length shrinkage ranging from 4.2% in R. hastula to 29.2% in the long morph of C. hromadkai and width shrinkage ranging from 1.1% in C. fryntai to 31.9% in the long morph of C. hromadkai. In addition, there is an apparent disparity between shrinkage of the cell length and cell width in some species. The most notable example is C. elongata, whose length decreases in protargol preparations by 22.9%, but its width increases by 12.5%. The GLM model revealed the significant effect of species (F16,840 = 361.26, P <0.001), cell condition (F1,840 = 343.92, P <0.001), and their interaction (F16,840 = 4.5, P <0.001) on the cell length. The effect of species (F16,827 = 143.98, P <0.001), cell condition (F1,827 = 232.94, P <0.001), and their interaction (F16,827 = 4.85, P <0.001) on the cell width were also significant. As shrinkage is calculated directly from the cell measurements (i.e. cell length and width), the GLM model clearly indicates that the rate of shrinkage differs among species considerably. RMA regression did not reveal significant relationship between shrinkage and cell size either in length (R2 = 0.036, P = 0.466) or width (R2 = 0.143, P = 0.133). Nevertheless, a weak positive relationship between shrinkage and cell length (Supporting Information, Fig. S6) and a weak negative relationship between shrinkage and cell width (Supporting Information, Fig. S7) was found.
description: Published as part of Kotyk, Michael, Bourland, William A., Soviš, Matyáš, Méndez-Sánchez, Daniel, Škaloud, Pavel, Varadínová, Zuzana Kotyková & Čepička, Ivan, 2024, Morphology maưers: congruence of morphology and phylogeny in the integrative taxonomy of Clevelandellidae (Ciliophora: Armophorea) with description of six new species, pp. 1-51 in Zoological Journal of the Linnean Society 202 (1) on pages 30-32, DOI: 10.1093/zoolinnean/zlad154, http://zenodo.org/record/14541981