title: Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° ÑÐ¿Ð¾ÑÐ¾Ð±Ð° Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð±ÐµÐ»ÐºÐ¾Ð² Ð¸Ð· Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Chlorella vulgaris
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Development of a method for isolating proteins from Chlorella vulgaris
Creators: name: Ð§ÐµÑÐ½ÑÑÐµÐ²Ð°, ÐÑÐµÐ½Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Chlorella
Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ð¸
ÑÐµÑÐ¼ÐµÐ½ÑÐ°ÑÐ¸Ð²Ð½ÑÐ¹ Ð³Ð¸Ð´ÑÐ¾Ð»Ð¸Ð·
Ð±ÐµÐ»ÐºÐ¸
Ð»Ð¸Ð¿Ð¸Ð´Ñ
microalgae
enzymatic hydrolysis
proteins
lipids

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð±ÐµÐ»ÐºÐ¾Ð² Ð¸Ð· Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ð¸ Chlorella vulgaris. ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: â Â ÐÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°ÑÑ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ C. vulgaris Ð² Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ½ÑÑ ÑÑÐ»Ð¾Ð²Ð¸ÑÑ; â ÐÐ¾Ð´Ð¾Ð±ÑÐ°ÑÑ Ð¾Ð¿ÑÐ¸Ð¼Ð°Ð»ÑÐ½ÑÐµ ÑÑÐ»Ð¾Ð²Ð¸Ñ Ð´Ð»Ñ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð±ÐµÐ»ÐºÐ° Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÑÐµÑÐ¼ÐµÐ½ÑÐ½ÑÑ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð² (ÑÐ¾Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ðµ Ð³Ð¸Ð´ÑÐ¾Ð¼Ð¾Ð´ÑÐ»Ñ, Ð¿Ð¾Ð´Ð±Ð¾Ñ ÑÐµÑÐ¼ÐµÐ½ÑÐ½Ð¾Ð³Ð¾ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ° Ð¸ ÐµÐ³Ð¾ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸); â ÐÑÐµÐ½Ð¸ÑÑ Ð°Ð¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾ÑÐ½ÑÐ¹ ÑÐ¾ÑÑÐ°Ð², Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð±ÐµÐ»ÐºÐ¾Ð²; â ÐÑÐ¾Ð²ÐµÑÑÐ¸ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ½Ð¾Ðµ Ð¸Ð·Ð²Ð»ÐµÑÐµÐ½Ð¸Ðµ ÑÐµÐ½Ð½ÑÑ ÐºÐ¾Ð¼Ð¿Ð¾Ð½ÐµÐ½ÑÐ¾Ð² Ð¸Ð· Chlorellavulgaris. ÐÐ±ÑÐµÐºÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â Ð±Ð¸Ð¾Ð¼Ð°ÑÑÐ° ChlorellaÑÐ¾ÑÐ³Ð¾Ð²Ð¾Ð¹ Ð¼Ð°ÑÐºÐ¸ Â«GroseriÂ», Ð±Ð¸Ð¾Ð¼Ð°ÑÑÐ° ChlorellavulgarisBIN, Ð¾ÑÑÐ°ÑÐ¾ÑÐ½Ð°Ñ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÐ° ChlorellavulgarisBINÂ Ð¿Ð¾ÑÐ»Ðµ Ð¸Ð·Ð²Ð»ÐµÑÐµÐ½Ð¸Ñ Ð»Ð¸Ð¿Ð¸Ð´Ð½Ð¾Ð³Ð¾ Ð¸ ÑÐµÐ½Ð¾Ð»ÑÐ½Ð¾Ð³Ð¾ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ°. Ð Ð°Ð±Ð¾ÑÐ° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ Ð±Ð¸Ð¾ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¸ ÐÐ¨ÐÐ¸ÐÐ ÐÐÐ¡Ð¸Ð. ÐÐ½Ð°Ð»Ð¸Ð·Ð¸ÑÑÐµÐ¼ÑÐµ Ð¿Ð¾ÐºÐ°Ð·Ð°ÑÐµÐ»ÑÐ¼Ð¸ Ð²ÑÑÐ¾Ð´Ð° Ð±ÐµÐ»ÐºÐ¾Ð² â ÑÐ¾Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ðµ Ð´Ð¾Ð»Ð¸ ÑÐµÑÐ¼ÐµÐ½ÑÐ°ÑÐ¸Ð²Ð½ÑÑ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð², Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÑ Ð´Ð¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸Ñ ÑÐµÑÐ¼ÐµÐ½ÑÐ½ÑÑ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð², ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÐ° ÑÐµÑÐ¼ÐµÐ½ÑÐ°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ Ð³Ð¸Ð´ÑÐ¾Ð»Ð¸Ð·Ð°. Ð ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ ÑÑÑÐµÐºÑÐ¸Ð²Ð½ÑÐ¹ ÑÐµÑÐ¼ÐµÐ½ÑÐ°ÑÐ¸Ð²Ð½ÑÐ¹ Ð³Ð¸Ð´ÑÐ¾Ð»Ð¸Ð· Ð´Ð»Ñ Ð²ÑÑÐ¾Ð´Ð° Ð±ÐµÐ»ÐºÐ° Ð¸Ð· Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ð¸ Chlorella vulgaris Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ Ð´Ð¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸Ñ ÑÐµÑÐ¼ÐµÐ½ÑÐ½ÑÑ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð²: Ð¦ÐµÐ»Ð»Ð¾ÐÑÐºÑ-Ð Ð¸ ÐÑÐ¾ÑÐ¾ÑÑÐ±ÑÐ¸Ð»Ð¸Ð½ Ð3Ñ Ð¿Ð¾ÑÐ»Ðµ Ð¸Ð·Ð²Ð»ÐµÑÐµÐ½Ð¸Ñ Ð¸Ð· Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ð¸ Ð»Ð¸Ð¿Ð¸Ð´Ð½Ð¾Ð³Ð¾ Ð¸ ÑÐµÐ½Ð¾Ð»ÑÐ½Ð¾Ð³Ð¾ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ°. ÐÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð¾ Ð±ÐµÐ»ÐºÐ¾Ð² ÑÐ¾ÑÑÐ°Ð²Ð¸Ð»Ð¾ â 125 Ð¼Ð³/Ð¼Ð».
description: This work is devoted to the study of protein isolation from the microalgae Chlorella vulgaris. Tasks that were solved in the course of the study: â Cultivate C. vulgaris biomass under laboratory conditions; â Select the optimal conditions for protein isolation using enzyme preparations (hydromodulus ratio, selection of an enzyme preparation and its concentration); â Assess the amino acid composition of the obtained proteins; â Carry out a complex extraction of valuable components from Chlorella vulgaris. The object of the study is the biomass of Chlorella of the Â«GroseriÂ» trademark, the biomass of Chlorella vulgaris BIN, the residual biomass of Chlorella vulgaris BIN after the extraction of the lipid and phenolic complex. The work was carried out on the basis of the laboratory of biotechnology of the Â Graduate School of Biotechnology and Food Production of the IBSiB. Analyzed by indicators of protein yield â the ratio of the proportion of enzymatic preparations, the sequence of adding enzyme preparations, the temperature of enzymatic hydrolysis. The study showed that the most effective enzymatic hydrolysis for the release of protein from microalgae Chlorella vulgaris using the sequential addition of enzyme preparations: CelloLux-A and Protosubtilin G3x after extracting the lipid and phenolic complex from microalgae. The amount of proteins was 125 mg/ml.Â