title: ÐÐµÐ³ÑÐ°Ð´Ð°ÑÐ¸Ñ ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° ÑÐ¿Ð¸Ð´ÐµÑÐ¼Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÐ°ÐºÑÐ¾ÑÐ° ÑÐ¾ÑÑÐ° Ð¸ Ð°ÑÑÐ¾ÑÐ°Ð³Ð¸Ñ Ð² Ð¾Ð¿ÑÑ Ð¾Ð»ÐµÐ²ÑÑ Ð¸ Ð½Ð¾ÑÐ¼Ð°Ð»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Degradation of the epidermal growth factor receptor and autophagy in human tumor and normal cells
Creators: name: ÐÐ¸ÑÐ°ÑÐµÐ½Ð¾Ðº, ÐÐ°ÑÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ð°Ð»ÑÐ½ÑÐµ Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð°Ð»ÑÐ½ÑÐµ ÑÑÑÐ¾Ð¼Ð°Ð»ÑÐ½ÑÐµ ÐºÐ»ÐµÑÐºÐ¸ (ÑÐ½ÐÐ¡Ð)
Ð°ÑÑÐ¾ÑÐ°Ð³Ð¸Ñ
ÑÐµÑÐµÐ¿ÑÐ¾Ñ ÑÐ¿Ð¸Ð´ÐµÑÐ¼Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÐ°ÐºÑÐ¾ÑÐ° ÑÐ¾ÑÑÐ° (Ð Ð­Ð¤Ð )
ÑÐ¿Ð¸Ð´ÐµÑÐ¼Ð°Ð»ÑÐ½ÑÐ¹ ÑÐ°ÐºÑÐ¾Ñ ÑÐ¾ÑÑÐ° (Ð­Ð¤Ð )
ÑÐ½Ð´Ð¾ÑÐ¸ÑÐ¾Ð·
ÐºÐ»ÐµÑÐºÐ¸ HeLa
endometrial mesenchymal stromal cells (enMSCs)
autophagy
epidermal growth factor receptor (EGFR)
epidermal growth factor (EGF)
endocytosis
HeLa cells

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description: The aim of the work was to study the level of autophagy in human endometrial mesenchymal stem cells (enMSCs) under various cultivation conditions and its effect on degradations of the epidermal growth factor receptor. To solve the research tasks, the methods of fluorescent confocal microscopy, SDS electrophoresis and immunoblotting were used. It has been shown that serum deprivation in our experimental conditions stimulates autophagy in human endometrial mesenchymal stem cells (enMSC) and in human cervical carcinoma tumor cells of the HeLa line, however, autophagy is stimulated to a much greater extent in enMSC. It was revealed that the process of autophagy in enMSC is stimulated not only by deprivation of serum in the culture medium, but also by changing the medium during cell cultivation. It was also found that in enMSC cells both of these factors stimulating autophagy process lead to a slowdown in the degradation of the epidermal growth factor receptor. Thus, we have demonstrated for the first time that generally accepted experimental schemes with a change of environment are able to stimulate autophagy in enMSC. The results of our work allow us to expand our understanding of the features of mesenchymal stem cells. Since MSCs are very promising objects for the treatment of a wide range of diseases, a comprehensive study of their properties and functions is of great practical importance.