title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ ÑÐ°Ð½Ð½Ð¸Ñ Ð±ÐµÐ»ÐºÐ¾Ð² Gp10 Ð¸ Gp20 Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð° phiKZ Ð½Ð° ÐºÑÐ»ÑÑÑÑÑ Escherichia coli Ð¸ Pseudomonas aeruginosa
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: The effect of early proteins Gp10 and Gp20 of the bacteriophage phiKZ on cell cultures of Escherichia Coli and Pseudomonas Aeruginosa
Creators: name: ÐÑÐ¾Ð·Ð´Ð¾Ð²Ð°, ÐÐ²Ð³ÐµÐ½Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³ phiKZ
ÑÐ°Ð½Ð½Ð¸Ðµ Ð³ÐµÐ½Ñ
Ð°Ð½ÑÐ¸Ð±Ð¸Ð¾ÑÐ¸ÐºÐ¾ÑÐµÐ·Ð¸ÑÑÐµÐ½ÑÐ½Ð¾ÑÑÑ
bacteriophage phiKZ
early genes
antibiotic resistance

description: ÐÐ°Ð´Ð°ÑÐ¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: â Ð¡ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÐºÐ»Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¿Ð¾Ð»ÑÑÐ¸ÑÑ Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð´Ð½ÑÐµ Ð²ÐµÐºÑÐ¾ÑÐ° Ñ Ð³ÐµÐ½Ð°Ð¼Ð¸ gp10 Ð¸ gp20 Ð´Ð»Ñ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐµÐ³Ð¾ ÑÐ¸Ð½ÑÐµÐ·Ð° Ð² Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²ÑÑÑÐ¸Ñ Ð±ÐµÐ»ÐºÐ¾Ð². â ÐÑÐ¾Ð°Ð½Ð°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°ÑÑ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð±ÐµÐ»ÐºÐ¾Ð² ÑÐ°Ð³Ð° Ð½Ð° ÑÐ¾ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ½ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð² Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ E. coli Ð¸ P. aeruginosa. â Ð£ÑÑÐ°Ð½Ð¾Ð²Ð¸ÑÑ Ð¼Ð¸ÑÐµÐ½Ñ Ð²Ð¾Ð·Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ð±ÐµÐ»ÐºÐ° Gp10. ÐÐ±ÑÐµÐºÑÐ°Ð¼Ð¸ Ð´Ð°Ð½Ð½Ð¾Ð¹ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÑÐºÐ¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¸ Ð²ÑÐ±ÑÐ°Ð½Ñ ÑÐ°Ð½Ð½Ð¸Ðµ Ð±ÐµÐ»ÐºÐ¸ Gp10 Ð¸ Gp20 Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð° phiKZ. ÐÑÐµÐ´Ð¼ÐµÑÐ¾Ð¼ Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð¼ÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ ÑÐ²Ð»ÑÐµÑÑÑ Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð±ÐµÐ»ÐºÐ¾Ð² Ð½Ð° Ð¶Ð¸Ð·Ð½ÐµÐ´ÐµÑÑÐµÐ»ÑÐ½Ð¾ÑÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð±Ð°ÐºÑÐµÑÐ¸Ð¹, Ð° ÑÐ°ÐºÐ¶Ðµ ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¸Ðµ Ð¸Ñ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½ÑÑ Ð¼Ð¸ÑÐµÐ½ÐµÐ¹ Ð²Ð¾Ð·Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ. ÐÑÐ¸Â  Ð½Ð°Ð¿Ð¸ÑÐ°Ð½Ð¸Ð¸Â  Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾Â  Ð¾Ð±Ð·Ð¾ÑÐ°Â  Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑÂ  ÑÐ»ÐµÐ´ÑÑÑÐ¸ÐµÂ  Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ: eLIBRARY.RU, NCBI. Ð¡ÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÐ°Ñ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÐ°Â  ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑÂ  Ð´Ð°Ð½Ð½ÑÑÂ  Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»Ð°ÑÑÂ  Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ð½Ð¾Ð³Ð¾ Ð¾Ð±ÐµÑÐ¿ÐµÑÐµÐ½Ð¸Ñ Origin, Ð¿Ð°ÐºÐµÑÐ° Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼ Microsoft Office Ð¸ ÑÐ»ÐµÐºÑÑÐ¾Ð½Ð½Ð¾Ð³Ð¾ ÑÐµÑÑÑÑÐ° The MEME Suite. Ð Ð°Ð±Ð¾ÑÐ°Â  Ð±ÑÐ»Ð°Â  Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð°Â  Ð½Ð°Â  Ð±Ð°Ð·ÐµÂ  Ð½Ð°ÑÑÐ½Ð¾-Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÑÐºÐ¾Ð³Ð¾ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ° Â«ÐÐ°Ð½Ð¾Ð±Ð¸Ð¾ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¸Â», ÑÐ¾Ð·Ð´Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð² ÑÐ¾ÑÑÐ°Ð²Ðµ Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¾Ð³Ð¾ Ð³Ð¾ÑÑÐ´Ð°ÑÑÑÐ²ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑÐ° ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾. Ð ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÑÐºÐ¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¾ Ð¿ÑÐ¾Ð°Ð½Ð°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°Ð½Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ ÑÐ°Ð½Ð½Ð¸Ñ Ð³ÐµÐ½Ð¾Ð² gp10 Ð¸ gp20 Ð½Ð° Ð¶Ð¸Ð·Ð½ÐµÐ´ÐµÑÑÐµÐ»ÑÐ½Ð¾ÑÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÑÐ°Ð¼Ð¼Ð° DH5Î± ÐºÑÐ»ÑÑÑÑÑ E. coli Ð¸ ÑÑÐ°Ð¼Ð¼Ð° PAO1 ÐºÑÐ»ÑÑÑÑÑ P. aeruginosa. Ð£ Ð¸Ð·ÑÑÐ°ÐµÐ¼ÑÑ Ð±ÐµÐ»ÐºÐ¾Ð² Ð±ÑÐ»Ð¾ ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¾ Ð½Ð°Ð»Ð¸ÑÐ¸Ðµ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÑÑÑÐµÐ³Ð¾ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ð½Ð° ÑÐ¾ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð¼Ð¸ÐºÑÐ¾Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð¾Ð². ÐÐ¾Ð»ÐµÐµ ÑÐ¸Ð»ÑÐ½ÑÐ¹ ÑÑÑÐµÐºÑ Ð½Ð°Ð±Ð»ÑÐ´Ð°Ð»ÑÑ Ð² Ð¾Ð¿ÑÑÐ°Ñ Ñ Ð±ÐµÐ»ÐºÐ¾Ð¼ Gp10. Ð Ð¿ÑÐ¾ÑÐµÑÑÐµ Ð¿Ð¾Ð¸ÑÐº Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð¹ Ð¼Ð¸ÑÐµÐ½Ð¸ Ð²Ð¾Ð·Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ ÑÐ°Ð½Ð½ÐµÐ³Ð¾ Ð±ÐµÐ»ÐºÐ° Gp10 ÑÐ°Ð³Ð° phiKZ Ð±ÑÐ»Ð¾ Ð²ÑÐ´Ð²Ð¸Ð½ÑÑÐ¾ Ð¿ÑÐµÐ´Ð¿Ð¾Ð»Ð¾Ð¶ÐµÐ½Ð¸Ðµ Ð¾ ÐµÐ³Ð¾ ÑÐ²ÑÐ·ÑÐ²Ð°Ð½Ð¸Ð¸ Ñ ÐÐÐ Ð±Ð°ÐºÑÐµÑÐ¸Ð¸ Ð½Ð° Ð½Ð°ÑÐ°Ð»ÑÐ½ÑÑ ÑÑÐ°Ð¿Ð°Ñ Ð¸Ð½ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ.
description: Research tasks: â Construct plasmid vectors with the gp10 and gp20 genes using genetic cloning methods for further synthesis of the respective proteins in bacterial cells. â Analyze the effect of phage proteins on laboratory strains of E. coli and P. aeruginosa cells. â Detect an impact target of Gp10 protein. The object of this research is the early proteins Gp10 and Gp20 of the bacteriophage phiKZ. The subject of this research is the study of the effect on the vital activity of bacterial cells provided by proteins, and detecting their possible impact targets. During writing the literary review, the following databases were used: eLIBRARY.RU, NCBI. The experimental data were processed using the Origin software, the software package Microsoft Office and The MEME Suite electronic resource. The work has been done on the basis of the scientific research complex Â«NanobiotechnologyÂ», created as part of Peter the Great St. Petersburg State Polytechnic University. During the researches, was analyzed the expression effect of gp10 and gp20 early genes on the vital activity of microorganisms â the DH5a strain of E. coli culture cells and the PAO1 strain of P. aeruginosa culture cells. It was found out that studied proteins have an inhibitory effect on the growth of microbial cells. A stronger effect was seen in experiments with the Gp10 protein. While searching of a potential impact target for the early protein Gp10 of phage phiKZ, it was detected that during the initial stages of infection it might binds to bacterial DNA.