Comparative evaluation of anti-apoptotic and anti-neuroglioproliferative activity of melatonin and citicoline on the retinal cells under contusion of the eye by flow cytometry analysis data
Creators: name: Komnatska, Kateryna
name: Chereshniuk, Ihor
name: Khodakivska, Olha
name: Khodakivskyi, O.
Publisher: name: figshare
Year: 2019
Subjects: subject: 70207 Humane Animal Treatment
subject: FOS: Animal and dairy science
subject: FOS: Animal and dairy science
subject: Pharmacology
subject: 111301 Ophthalmology
subject: FOS: Clinical medicine
subject: FOS: Clinical medicine

description: <b>Introduction.</b> In front of visual analyzer contusion after termination of an traumatic agent influence the locus of destructive degenerative retinal changes in retina is heterogeneous and non-homogenous, and is presented by apoptotic, necrotic and, in later period, by neuroglioproliferative events. It is possible to separate and characterize these changes in front of cytoprotector administration using the separate flow DNA cytometry of retinal cells suspension. <b>Purpose.</b> Using the flow cytometry analysis, to evaluate the influence of melatonin on the progress of apoptotic processes in retinal cells by presence of nuclear DNA, investigate its possible antineuroglioproliferative activity and compare the particular aspects with the alternative neuroprotector citicoline. <b>Materials and methods. </b>During modeling of contusion of an eye the pressurized carbon dioxide gas empty shot was applied on the center of cornea in narcotized rabbits. The first separate administration of investigational products (melatonin, 10 mg/kg and citicoline, 250 mg/kg) was performed in an hour, within an interval of 12 hours, during 7 consecutive days. The presence of nuclear DNA with fragmentation signs was the marker for nonreversible apoptosis, which was proved by flow cytometry analysis. The last also allowed to determine the presence of nuclear DNA with chromosome complement less than diploid (2n), that, considering analysis of somatic and not germinal cells allowed the evidential statement about apoptosis. The presence of neuroglioproliferative activity was determined by phase S of cell cycle, the appropriate period was outlined at DNA histograms and characterized percentage ratio of nuclei that were in the period of DNA synthesis with its content &gt; 2n and&lt; 4n to their total investigated quantity. <b>Results and discussion.</b> In front of separate intravenous administration of melatonin or citicoline in therapeutic effective doses of 10 and 250 mg/kg respectively to rabbits with modeled visual analyzer contusion, the level of antiapoptotic effect calculated on the basis of number of retinal cells with nuclear DNA that has signs of fragmentation, melatonin prevails the comparator drug in average by 1,55 times, р&lt;0,05. The same therapy for eye contusion in rabbits using investigational products avoided and localized the events of neuroglial proliferation which manifested by the significantly lower number of cells where DNA was is in the stage of fission along with prevalence of melatonin over citicoline in average by 1,55 times, р&lt;0,05. <b>Prospects for further research.</b> The obtained data are the basis for more comprehensive research of possibility for implementation of these medicinal products especially melatonin as the leading drug among the investigated assortment of organ protectors into practical ophthalmological practice for treatment of traumatic visual analyzer injuries of different genesis.