title: ÐÐ¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾-Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ°Ñ Ñ Ð°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ° Ð¸ Ð¿Ð°ÑÐ¾Ð³ÐµÐ½Ð½ÑÐ¹ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð» Ð°Ð½Ð°Ð¼Ð¾ÑÑÐ½ÑÑ Ð°ÑÐºÐ¾Ð¼Ð¸ÑÐµÑÐ¾Ð² ÐºÐ°Ðº Ð¿ÐµÑÑÐ¿ÐµÐºÑÐ¸Ð²Ð½ÑÑ Ð¿ÑÐ¾Ð´ÑÑÐµÐ½ÑÐ¾Ð² Ð±Ð¸Ð¾Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð²
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Molecular genetic characteristics and pathogenic potential of anamorphic ascomycetes as promising producers of biological products
Creators: name: Ð§ÐµÑÐµÐ¿Ð°Ð½Ð¾Ð²Ð°, ÐÐ°ÑÐ¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÐÐ¸Ð¾ÑÐµÑ Ð½Ð¾Ð»Ð¾Ð³Ð¸Ñ
ÑÐ½ÑÐ¾Ð¼Ð¾Ð¿Ð°ÑÐ¾Ð³ÐµÐ½Ð½ÑÐµ Ð³ÑÐ¸Ð±Ñ
Ð¿Ð°ÑÐ¾Ð³ÐµÐ½Ð½Ð¾ÑÑÑ
ÑÐ¸ÑÑÐµÐ¼Ð°ÑÐ¸ÐºÐ°
Ð¼ÑÐ»ÑÑÐ¸Ð»Ð¾ÐºÑÑÐ½Ð¾Ðµ Ð³ÐµÐ½Ð¾ÑÐ¸Ð¿Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
Ð°Ð½ÑÐ°Ð³Ð¾Ð½Ð¸Ð·Ð¼
entomopathogenic fungi
pathogenicity
taxonomy
multilocus genotyping
antagonism

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«ÐÐ¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾-Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ°Ñ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ° Ð¸ Ð¿Ð°ÑÐ¾Ð³ÐµÐ½Ð½ÑÐ¹ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð» Ð°Ð½Ð°Ð¼Ð¾ÑÑÐ½ÑÑ Ð°ÑÐºÐ¾Ð¼Ð¸ÑÐµÑÐ¾Ð² ÐºÐ°Ðº Ð¿ÐµÑÑÐ¿ÐµÐºÑÐ¸Ð²Ð½ÑÑ Ð¿ÑÐ¾Ð´ÑÑÐµÐ½ÑÐ¾Ð² Ð±Ð¸Ð¾Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð²Â». ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐµ Ð¿Ð¾ Ð²Ð¸Ð´Ð¾Ð²Ð¾Ð¹ Ð¿ÑÐ¸Ð½Ð°Ð´Ð»ÐµÐ¶Ð½Ð¾ÑÑÐ¸ Ð¸ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð½Ð¾Ð²ÑÑ Ð¿ÑÐ¸ÑÐ¾Ð´Ð½ÑÑ Ð¸Ð·Ð¾Ð»ÑÑÐ¾Ð² Lecanicillium-Ð¿Ð¾Ð´Ð¾Ð±Ð½ÑÑ Ð³ÑÐ¸Ð±Ð¾Ð² Ð¸ Ð²ÑÑÐ²Ð»ÐµÐ½Ð¸Ñ Ð¿ÐµÑÑÐ¿ÐµÐºÑÐ¸Ð²Ð½ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð² Ð´Ð»Ñ ÑÐ¾Ð·Ð´Ð°Ð½Ð¸Ñ Ð±Ð¸Ð¾Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð² Ð·Ð°ÑÐ¸ÑÑ ÑÐ°ÑÑÐµÐ½Ð¸Ð¹. ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: 1. ÐÐ·ÑÑÐµÐ½Ð¸Ðµ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¾-ÐºÑÐ»ÑÑÑÑÐ°Ð»ÑÐ½ÑÑ ÑÐ²Ð¾Ð¹ÑÑÐ² Ð½Ð¾Ð²ÑÑ Ð¿ÑÐ¸ÑÐ¾Ð´Ð½ÑÑ Ð¸Ð·Ð¾Ð»ÑÑÐ¾Ð² Lecanicillium-Ð¿Ð¾Ð´Ð¾Ð±Ð½ÑÑ Ð³ÑÐ¸Ð±Ð¾Ð² Ð¸Ð· ÐºÐ¾Ð»Ð»ÐµÐºÑÐ¸Ð¸ ÐÐÐÐ ; 2. ÐÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ðµ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾Ð¹ Ð¸Ð´ÐµÐ½ÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Ð¸Ð·ÑÑÐ°ÐµÐ¼ÑÑ Ð¿ÑÐ¸ÑÐ¾Ð´Ð½ÑÑ Ð¸Ð·Ð¾Ð»ÑÑÐ¾Ð² Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¼ÑÐ»ÑÑÐ¸Ð»Ð¾ÐºÑÑÐ½Ð¾Ð³Ð¾ Ð³ÐµÐ½Ð¾ÑÐ¸Ð¿Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ; 3. Ð¤Ð¸Ð»Ð¾Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð°Ð½Ð°Ð»Ð¸Ð· Ð¸ Ð²ÑÑÐ²Ð»ÐµÐ½Ð¸Ðµ ÑÐ²ÑÐ·Ð¸ Ð¼ÐµÐ¶Ð´Ñ Ð¸Ð·ÑÑÐ°ÐµÐ¼ÑÐ¼Ð¸ Ð¸Ð·Ð¾Ð»ÑÑÐ°Ð¼Ð¸; 4. ÐÐ·ÑÑÐµÐ½Ð¸Ðµ Ð¿Ð°ÑÐ¾Ð³ÐµÐ½Ð½ÑÑ ÑÐ²Ð¾Ð¹ÑÑÐ² Ð¸Ð·Ð¾Ð»ÑÑÐ¾Ð² Ð² Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ð¸ MyzusÂ persicae; 5. ÐÑÐµÐ½ÐºÐ° Ð°Ð½ÑÐ°Ð³Ð¾Ð½Ð¸ÑÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð¸Ð·Ð¾Ð»ÑÑÐ¾Ð² Ð² Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ð¸ ÑÐ¸ÑÐ¾Ð¿Ð°ÑÐ¾Ð³ÐµÐ½Ð½ÑÑ Ð¼Ð¸ÐºÑÐ¾Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð¾Ð². Ð Ð°Ð±Ð¾ÑÐ° Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»Ð°ÑÑ Ð½Ð° Ð±Ð°Ð·Ðµ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ Ð¼Ð¸ÐºÑÐ¾Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð·Ð°ÑÐ¸ÑÑ ÑÐ°ÑÑÐµÐ½Ð¸Ð¹ Â«Ð¤ÐµÐ´ÐµÑÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð³Ð¾ÑÑÐ´Ð°ÑÑÑÐ²ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð±ÑÐ´Ð¶ÐµÑÐ½Ð¾Ð³Ð¾ Ð½Ð°ÑÑÐ½Ð¾Ð³Ð¾ ÑÑÑÐµÐ¶Ð´ÐµÐ½Ð¸Ñ âÐÑÐµÑÐ¾ÑÑÐ¸Ð¹ÑÐºÐ¸Ð¹ Ð½Ð°ÑÑÐ½Ð¾-Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÑÐºÐ¸Ð¹ Ð¸Ð½ÑÑÐ¸ÑÑÑ Ð·Ð°ÑÐ¸ÑÑ ÑÐ°ÑÑÐµÐ½Ð¸Ð¹âÂ», Ð³Ð´Ðµ Ð±ÑÐ»Ð¸ ÑÐ¾Ð±ÑÐ°Ð½Ñ Ð²ÑÐµ ÑÐ°ÐºÑÐ¸ÑÐµÑÐºÐ¸Ðµ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ñ: ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð¼ÑÐ»ÑÑÐ¸Ð»Ð¾ÐºÑÑÐ½Ð¾Ð³Ð¾ Ð³ÐµÐ½Ð¾ÑÐ¸Ð¿Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸ÑÂ  Lecanicillium-Ð¿Ð¾Ð´Ð¾Ð±Ð½ÑÑ Ð³ÑÐ¸Ð±Ð¾Ð², Ð¸Ñ Ð²Ð¸ÑÑÐ»ÐµÐ½ÑÐ½Ð¾ÑÑÑ Ð² Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ð¸ MyzusÂ persicae Ð¸ Ð¸Ñ Ð°Ð½ÑÐ°Ð³Ð¾Ð½Ð¸ÑÑÐ¸ÑÐµÑÐºÐ¸Ðµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð°. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð¸Ð´ÐµÐ½ÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ñ 16-Ð¸ Ð¸Ð·Ð¾Ð»ÑÑÐ¾Ð² Lecanicillium-Ð¿Ð¾Ð´Ð¾Ð±Ð½ÑÑ Ð³ÑÐ¸Ð±Ð¾Ð². Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¿Ð°ÑÐ¾Ð³ÐµÐ½Ð½ÑÑ Ð¸ ÑÑÐ½Ð³Ð¸ÑÑÐ°ÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÐ²Ð¾Ð¹ÑÑÐ² Ð²ÑÑÐ²Ð»ÐµÐ½ Ð¸Ð·Ð¾Ð»ÑÑ AkÂ 13 Ð²Ð¸Ð´Ð° Flavocillium bifurcatum ÐºÐ°Ðº Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ Ð¿ÐµÑÑÐ¿ÐµÐºÑÐ¸Ð²Ð½ÑÐ¹ Ð´Ð»Ñ ÑÐ¾Ð·Ð´Ð°Ð½Ð¸Ñ Ð±Ð¸Ð¾Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ° Ð´Ð»Ñ Ð·Ð°ÑÐ¸ÑÑ ÑÐ°ÑÑÐµÐ½Ð¸Ð¹, Ð¾Ð±Ð»Ð°Ð´Ð°ÑÑÐµÐ³Ð¾ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ½ÑÐ¼ Ð´ÐµÐ¹ÑÑÐ²Ð¸ÐµÐ¼.
description: The subject of the graduate qualification work is âMolecular genetic characteristics and pathogenic potential of anamorphic ascomycetes as promising producers of biological productsâ. This work is devoted to the characterization by species and biological activity of new natural isolates of Lecanicillium-like fungi and the identification of promising strains for the creation of plant protection biologics. The research set the following goals: 1. Study of morphological and cultural properties of new natural isolates of Lecanicillium-like fungi from the collection of VIZR; 2. Carrying out the molecular identification of the studied natural isolates using multilocus genotyping; 3. Phylogenetic analysis and identification of the relationship between the studied isolates; 4. Study of pathogenic properties of isolates against Myzus persicae; 5. Evaluation of the antagonistic activity of isolates against phytopathogenic microorganisms. The work was carried out on the basis of the Laboratory of Microbiological Plant Protection of the Federal State Budgetary Scientific Institution "All-Russian Research Institute of Plant Protection", where all the actual materials were collected: results of multilocus genotyping of Lecanicillium-like fungi, their virulence against Myzus persicae and their antagonistic properties. As a result of the work, 16 isolates of Lecanicillium-like fungi were identified. As a result of the determination of pathogenic and fungistatic properties, the isolate Ak13 of the Flavocillium bifurcatum species was identified as the most promising for the creation of a biological product for plant protection with a complex effect.