cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Doubling Expression of the Low Density Lipoprotein Receptor by Truncation of the 3′-Untranslated Region Sequence Ameliorates Type III Hyperlipoproteinemia in Mice Expressing the Human ApoE2 Isoform
Creators: name: Malloy, Sudi
name: Maeda, Nobuyo
name: Wilder, Jennifer
name: Altenburg, Michael K.
name: Knouff, Christopher
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2001

description: The primary receptor mediating clearance of apolipoprotein (apo)E- and apoB100-containing lipoproteins from the circulation is the low density lipoprotein (LDL) receptor. Reduced expression of the LDLR is believed to be a precipitating factor in the pathogenesis of type III hyperlipoproteinemia (HLP) in some humans homozygous for the apoE2 allele (APOE*2). To test the effect of genetic changes in LDL receptor expression on the pathogenesis of type III HLP, we have generated a variant allele at the endogenous mouse Ldlr locus that expresses the human LDL receptor transcript. Transcription of the human LDLR minigene is regulated by the endogenous mouse promoter sequence, but a truncation of 3'-untranslated region results in increased mRNA stability. Consequently, in liver of heterozygotes, steady state levels of mouse and human LDLR transcripts are 50 and 180% the levels of total transcript in wild type mice, respectively. Overall, the 2.3-fold normal level of LDLR message in heterozygotes completely ameliorates type III HLP caused by the homozygosity for the human APOE*2 allele, normalizing their plasma lipoprotein profile. We conclude that a modest increase in expression of the LDLR through message stabilization is sufficient to prevent precipitation of type III HLP in mice.
Expression and Characterization of Recombinant Rat Acyl-CoA Synthetases 1, 4, and 5: SELECTIVE INHIBITION BY TRIACSIN C AND THIAZOLIDINEDIONES
Creators: name: Lewin, Tal M.
name: Kim, Ji Hyeon
name: Coleman, Rosalind A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2001

description: Inhibition by triacsins and troglitazone of long chain fatty acid incorporation into cellular lipids suggests the existence of inhibitor-sensitive and -resistant acyl-CoA synthetases (ACS, EC ) that are linked to specific metabolic pathways. In order to test this hypothesis, we cloned and purified rat ACS1, ACS4, and ACS5, the isoforms present in liver and fat cells, expressed the isoforms as ACS-Flag fusion proteins in Escherichia coli, and purified them by Flag affinity chromatography. The Flag epitope at the C terminus did not alter the kinetic properties of the enzyme. Purified ACS1-, 4-, and 5-Flag isoforms differed in their apparent K(m) values for ATP, thermolability, pH optima, requirement for Triton X-100, and sensitivity to N-ethylmaleimide and phenylglyoxal. The ACS inhibitor triacsin C strongly inhibited ACS1 and ACS4, but not ACS5. The thiazolidinedione (TZD) insulin-sensitizing drugs and peroxisome proliferator-activated receptor gamma (PPARgamma) ligands, troglitazone, rosiglitazone, and pioglitazone, strongly and specifically inhibited only ACS4, with an IC(50) of less than 1.5 microm. Troglitazone exhibited a mixed type inhibition of ACS4. alpha-Tocopherol, whose ring structure forms the non-TZD portion of troglitazone, did not inhibit ACS4, indicating that the thiazolidine-2,4-dione moiety is the critical component for inhibition. A non-TZD PPARgamma ligand, GW1929, which is 7-fold more potent than rosiglitazone, inhibited ACS1 and ACS4 poorly with an IC(50) of greater than 50 microm, more than 100-fold higher than was required for rosiglitazone, thereby demonstrating the specificity of TZD inhibition. Further, the PPARalpha ligands, clofibrate and GW4647, and various xenobiotic carboxylic acids known to be incorporated into complex lipids had no effect on ACS1, -4, or -5. These results, together with previous data showing that triacsin C and troglitazone strongly inhibit triacylglycerol synthesis compared with other metabolic pathways, suggest that ACS1 and ACS4 catalyze the synthesis of acyl-CoAs used for triacylglycerol synthesis and that lack of inhibition of a metabolic pathway by triacsin C does not prove lack of acyl-CoA involvement. The results further suggest the possibility that the insulin-sensitizing effects of the thiazolidinedione drugs might be achieved, in part, through direct interaction with ACS4 in a PPARgamma-independent manner.
(1783) Ida Fynes-Clinton
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
F Plasmid Conjugative DNA Transfer: THE TraI HELICASE ACTIVITY IS ESSENTIAL FOR DNA STRAND TRANSFER
Creators: name: Byrd, Devon R.N.
name: Sampson, Juliana K.
name: Matson, Steven W.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2001

description: The product of the Escherichia coli F plasmid traI gene is required for DNA transfer via bacterial conjugation. This bifunctional protein catalyzes the unwinding of duplex DNA and is a sequence-specific DNA transesterase. The latter activity provides the site- and strand-specific nick required to initiate DNA transfer. To address the role of the TraI helicase activity in conjugative DNA transfer traI mutants were constructed and their function in DNA transfer was evaluated using genetic and biochemical methods. A traI deletion/insertion mutant was transfer-defective as expected. A traI C-terminal deletion that removed the helicase-associated motifs was also transfer-defective despite the fact that the region of traI encoding the transesterase activity was intact. Biochemical studies demonstrated that the N-terminal domain was sufficient to catalyze oriT-dependent transesterase activity. Thus, a functional transesterase was not sufficient to support DNA transfer. Finally, a point mutant, TraI-K998M, that lacked detectable helicase activity was characterized. This protein catalyzed oriT-dependent transesterase activity in vitro and in vivo but failed to complement a traI deletion strain in conjugative DNA transfer assays. Thus, both the transesterase and helicase activities of TraI are essential for DNA strand transfer.
(1785) John Blakiston-Houston
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Time Evolution of the CMB Quadrupole
Creators: name: Loeb, Abraham
Publisher: name: arXiv
Year: 2022
Subjects: subject: Cosmology and Nongalactic Astrophysics (astro-ph.CO)
subject: FOS: Physical sciences

description: I show that the quadrupole of the Cosmic Microwave Background (CMB) evolves more rapidly than previously expected, as a result of the acceleration of the Sun towards the Galactic center. The acceleration, measured most recently by Gaia EDR3, implies a fractional change in the quadrupole of ~10^{-9} per year, an order of magnitude larger than expected from the evolution in the last scattering surface of the CMB.
description: 3 pages, submitted to an AAS Journal
D1.4 Characterisation of Geo-referenced Datasets
Creators: name: STAM Srl
name: University Of Parma
name: Aristotle University Of Thessaloniki
name: Cooperativas Agro-Alimentarias
Publisher: name: Zenodo
Year: 2022
Subjects: AGRICORE H2020

description: This deliverable presents the methodology defined within the AGRICORE project to characterise geo-referenced datasets. This methodology has been developed as part of the first of the work packages defined in the AGRICORE project aiming to characterise data sources useful for performing agricultural research analysis, and has been adapted to the characterisation of georeferenced datasets within T1.4. AGRICORE is a research project proposing an innovative way to apply agent-based modelling to improve the capacities of policymakers to evaluate the impact of agricultural-related measurements under and outside the framework of the Common Agricultural Policy. This project was funded by the European Commission as a result of the RUR04-2018 call, part of the H2020 programme. Introduction presents the basis on which the methodology has been developed i.e., the ARDIT tool and the AGRICORE DCAT-AP 2.0 ontology. Then, the proposed methodology is detailed including also the definitions of all the fields to be filled while characterising a dataset in ARDIT. As a part of this methodology, also all the main challenges encountered during the characterisation of geo-referenced datasets have been explained, illustrating the proposed solutions. Also the methodology proposed to guarantee that the tool ARDIT remains efficiently working is described, relying on a continuous upgrade and maintenance of the data. Finally, some conclusions regarding the characterisation process and the need for a governance to ensure the survival of the ARDIT after the project are provided. It is important to remark that although this deliverable has been developed in the framework of the AGRICORE project, the participating partners have aimed broader usage of the proposed methodology. As the final goal of this work package, the proposed EU Index Tool (now renamed as Agricultural Research Data Index Tool (ARDIT) aims to serve as a central entry point for locating useful datasets for agricultural research.
Gβ Association and Effector Interaction Selectivities of the Divergent Gγ Subunit Gγ 13
Creators: name: Siderovski, David P.
name: Bayliss, Douglas A.
name: Lei, Qiubo
name: Morris, Rebecca A.
name: Hillmann, Jennie R.
name: Miller, Richard J.
name: Zhou, Janice Y.
name: Wing, Michele R.
name: Blake, Bonita L.
name: Harden, T. Kendall
name: Behe, Cynthia I.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2001

description: G gamma(13) is a divergent member of the G gamma subunit family considered to be a component of the gustducin G-protein heterotrimer involved in bitter and sweet taste reception in taste bud cells. G gamma(13) contains a C-terminal asparagine-proline-tryptophan (NPW) tripeptide, a hallmark of RGS protein G gamma-like (GGL) domains which dimerize exclusively with G beta(5) subunits. In this study, we investigated the functional range of G gamma(13) assembly with G beta subunits using multiple assays of G beta association and G beta gamma effector modulation. G gamma(13) was observed to associate with all five G beta subunits (G beta(1-5)) upon co-translation in vitro, as well as function with all five G beta subunits in the modulation of Kir3.1/3.4 (GIRK1/4) potassium and N-type (alpha(1B)) calcium channels. Multiple G beta/G gamma(13) pairings were also functional in cellular assays of phospholipase C (PLC) beta 2 activation and inhibition of G alpha(q)-stimulated PLC beta 1 activity. However, upon cellular co-expression of G gamma(13) with different G beta subunits, only G beta(1)/G gamma(13), G beta(3)/G gamma(13), and G beta(4)/G gamma(13) pairings were found to form stable dimers detectable by co-immunoprecipitation under high-detergent cell lysis conditions. Collectively, these data indicate that G gamma(13) forms functional G beta gamma dimers with a range of G beta subunits. Coupled with our detection of G gamma(13) mRNA in mouse and human brain and retina, these results imply that this divergent G gamma subunit can act in signal transduction pathways other than that dedicated to taste reception in sensory lingual tissue.
Hrs Interacts with Sorting Nexin 1 and Regulates Degradation of Epidermal Growth Factor Receptor
Creators: name: Raynor, Mathew C.
name: Li, Lian
name: Chin, Lih Shen
name: Chen, Hui Qi
name: Wei, Xiaolong
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2001

description: Hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is a mammalian homologue of yeast vacuolar protein sorting (Vps) protein Vps27p; however, the role of Hrs in lysosomal trafficking is unclear. Here, we report that Hrs interacts with sorting nexin 1 (SNX1), a recently identified mammalian homologue of yeast Vps5p that recognizes the lysosomal targeting code of epidermal growth factor receptor (EGFR) and participates in lysosomal trafficking of the receptor. Biochemical analyses demonstrate that Hrs and SNX1 are ubiquitous proteins that exist in both cytosolic and membrane-associated pools, and that the association of Hrs and SNX occurs on cellular membranes but not in the cytosol. Furthermore, endogenous SNX1 and Hrs form a approximately 550-kDa complex that excludes EGFR. Immunofluorescence and subcellular fractionation studies show that Hrs and SNX1 colocalize on early endosomes. By using deletion analysis, we have mapped the binding domains of Hrs and SNX1 that mediate their association. Overexpression of Hrs or its SNX1-binding domain inhibits ligand-induced degradation of EGFR, but does not affect either constitutive or ligand-induced receptor-mediated endocytosis. These results suggest that Hrs may regulate lysosomal trafficking through its interaction with SNX1.
Human Cytosolic 5′-Nucleotidase I: CHARACTERIZATION AND ROLE IN NUCLEOSIDE ANALOG RESISTANCE
Creators: name: Hunsucker, Sally Anne
name: Spychala, Jozef
name: Mitchell, Beverly S.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2001

description: Nucleoside analogs are important in the treatment of hematologic malignancies, solid tumors, and viral infections. Their metabolism to the triphosphate form is central to their chemotherapeutic efficacy. Although the nucleoside kinases responsible for the phosphorylation of these compounds have been well described, the nucleotidases that may mediate drug resistance through dephosphorylation remain obscure. We have cloned and characterized a novel human cytosolic 5'-nucleotidase (cN-I) that potentially may have an important role in nucleoside analog metabolism. It is expressed at a high level in skeletal and heart muscle, at an intermediate level in pancreas and brain, and at a low level in kidney, testis, and uterus. The recombinant cN-I showed high affinity toward dCMP and lower affinity toward AMP and IMP. ADP was necessary for maximal catalytic activity. Expression of cN-I in Jurkat and HEK 293 cells conferred resistance to 2-chloro-2'-deoxyadenosine, with a 49-fold increase in the IC(50) in HEK 293 and a greater than 400-fold increase in the IC(50) in Jurkat cells. Expression of cN-I also conferred a 22-fold increase in the IC(50) to 2',3'-difluorodeoxycytidine in HEK 293 cells and an 82-fold increase in the IC(50) to 2',3'-dideoxycytidine in Jurkat cells. These data indicate that cN-I may play an important role in the regulation of physiological pyrimidine nucleotide pools and may also alter the therapeutic efficacy of certain nucleoside analogs.
Imprint of a 2 Myr old source on the cosmic ray anisotropy
Creators: name: Savchenko, V.
name: Kachelriess, M.
name: Semikoz, D. V.
Publisher: name: arXiv
Year: 2015
Subjects: subject: High Energy Astrophysical Phenomena (astro-ph.HE)
subject: FOS: Physical sciences

description: We study numerically the anisotropy of the cosmic ray (CR) flux emitted by a single source calculating the trajectories of individual CRs. We show that the contribution of a single source to the observed anisotropy is instead determined solely by the fraction the source contributes to the total CR intensity, its age and its distance,and does not depend on the CR energy at late times. Therefore the observation of a constant dipole anisotropy indicates that a single source dominates the CR flux in the corresponding energy range. A natural explanation for the plateau between 2--20 TeV observed in the CR anisotropy is thus the presence of a single, nearby source. For the source age of 2 Myr, as suggested by the explanation of the antiproton and positron data from PAMELA and AMS-02 through a local source [arXiv:astro-ph/1504.06472], we determine the source distance as $\sim 200$ pc. Combined with the contribution of the global CR sea calculated in the escape model, we can explain qualitatively the data for the dipole anisotropy. Our results suggest that the assumption of a smooth CR source distribution should be abandoned between 200 GeV and 1 PeV.
description: 4 pages, 4 eps figures; v2: minor changes, to appear in ApJL
The effects of binary interactions on parameter determinations for early-type galaxies
Creators: name: Zhang, Yu
name: Liu, Jinzhong
name: Zhang, Fenghui
Publisher: name: arXiv
Year: 2015
Subjects: subject: Astrophysics of Galaxies (astro-ph.GA)
subject: FOS: Physical sciences

description: Based on stellar population models without (SSP) and with (BSP) binary interactions, we investigate the effects of binary interactions on parameter determinations for early-type galaxies (ETGs). We present photometric redshift (photo-z), age and spectral type for photometric data sample by fitting observed magnitudes with the SSP and BSP models. Our results show that binary interactions have no effect on photo-z estimation. Once we neglect binary interactions, the age of ETGs will be underestimated, by contrast, the effects on the age estimations can be negligible for other type of galaxies. For ETG sample, we derive their properties by fitting their spectra with the SSP and BSP models. When comparing these galaxy properties, we find no variation of the overall metallicities for ETGs among the SSP and BSP models. Moreover, the inclusion of binary interactions can affect age estimations. Our results show that the BSP-fitted ages in ~33.3% of ETG sample are around 0.5-1.0 Gyr larger than the SSP-fitted ages; ~44.2\% are only 0.1-0.5 Gyr larger; the rest ~22.5% are approximately equal. By comparisons, we find the difference of the star formation rate between the SSP and BSP models is large at the late evolution stage.
Wundermittel Resilienz? Resilienzförderung im Arbeitsumfeld
Creators: name: Riesen, Deborah
Publisher: name: Zenodo
Year: 2022

description: Arbeit wird mit Sinnhaftigkeit, Struktur und Teilhabe in Verbindung gebracht. Für viele Menschen bedeutet Arbeit jedoch auch Belastung und Stress. Zum Schutz der psychischen Gesundheit bei Arbeitsbelastungen ist Resilienz als Konzept zunehmend populär geworden. Es gilt daher zu überprüfen, inwiefern sich die Resilienzförderung auf die psychische Gesundheit von Mitarbeitenden, in der sich stetig verändernden Arbeitswelt, auswirkt. Dazu wurden in der vorliegenden Master-Thesis im Rahmen einer systematischen Literaturrecherche theoretische Grundlagen zu psychischer Gesundheit, Arbeitswelt und Arbeitsbedingungen sowie dem Resilienzkonzept erarbeitet. Anschliessend wurden Resilienzprogramme in der Arbeitswelt systematisch ausgewertet und verglichen. Die Ergebnisse zeigen auf, dass die untersuchten Resilienzprogramme unzureichend konzeptualisiert und deren Wirksamkeit für die Resilienz sowie die psychische Gesundheit nicht genügend belegt ist. Künftig ist eine systematische und umfassende Resilienzforschung gefragt, damit darauf aufbauend nachhaltige Massnahmen entwickelt werden können. Resilienz als alleinige Lösung für den Umgang mit arbeitsbedingten Stressoren reicht allerdings nicht aus und die Verantwortung für die psychische Gesundheit darf nicht individualisiert werden. Aufgabe der Sozialen Arbeit ist eine kritische Auseinandersetzung mit bestehenden Arbeitsbedingungen und das Erstreben eines Arbeitsumfeldes, in welchem Menschen psychisch gesund bleiben können.
description: + Code Diss LU: hslusa masa be + Fussnote: Master of Science in Sozialer Arbeit, Bern, Luzern, St. Gallen, 2022 + NL-Code: NLLUHSA202209
Identification of Novel Pheromone-response Regulators through Systematic Overexpression of 120 Protein Kinases in Yeast
Creators: name: Burchett, Scott A.
name: Dohlman, Henrik G.
name: Scott, Anisa
name: Errede, Beverly
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2001

description: Protein kinases are well known to transmit and regulate signaling pathways. To identify additional regulators of the pheromone signaling apparatus in yeast, we evaluated an array of 120 likely protein kinases encoded by the yeast genome. Each kinase was fused to glutathione S-transferase, overexpressed, and tested for changes in pheromone responsiveness in vivo. As expected, several known components of the pathway (YCK1, STE7, STE11, FUS3, and KSS1) impaired the growth arrest response. Seven other kinases also interfered with pheromone-induced growth arrest; in rank order they are as follows: YKL116c (renamed PRR1) = YDL214c (renamed PRR2) > YJL141c (YAK1, SRA1) > YNR047w = YCR091w (KIN82) = YIL095w (PRK1) > YCL024w (KCC4). Inhibition of pheromone signaling by PRR1, but not PRR2, required the glutathione S-transferase moiety. Both kinases inhibited gene transcription after stimulation with pheromone, a constitutively active kinase mutant STE11-4, or overexpression of the transcription factor STE12. Neither protein altered the ability of the mitogen-activated protein kinase (MAPK) Fus3 to feedback phosphorylate a known substrate, the MAPK kinase Ste7. These results reveal two new components of the pheromone-signaling cascade in yeast, each acting at a point downstream of the MAPK.
Mutterzentrierung im Kindesschutz: Eine qualitativ-empirische Untersuchung des impliziten Wissens der Sozialarbeitenden in professionellen Entscheidungsprozessen von Kindeswohlgefährdungen
Creators: name: Steinmann, Selina
Publisher: name: Zenodo
Year: 2022

description: Im Tätigkeitsfeld des Kindesschutzes steht die Frage im Zentrum, ob das Kindeswohl in einem Familiensystem gefährdet ist oder nicht. Diesbezüglich müssen Fachpersonen der Sozialen Arbeit Entscheidungen und Urteile treffen. Die Leitmaxime eines solchen Entscheides bildet der unbestimmte Rechtsbegriff des Kindeswohles. Die vorliegende Master-Thesis nimmt Entscheidungsprozesse im Kindesschutz in den Fokus. Anhand eines explorativen Vorgehens werden dreizehn Abklärungsberichte eines Sozialdienstes operierend in einem urbanen Umfeld mit der dokumentarischen Methode analysiert. Dabei wird der Frage nachgegangen, welches implizite Wissen in professionellen Entscheidungsprozessen in Bezug auf das Kindeswohl erkennbar ist. Das Ergebnis zeigt anhand der Identifizierung verschiedener Orientierungsrahmen, dass einerseits die Entscheidungsprozesse vor dem Hintergrund der normativen Rollenbilder der familialisierten Kindheit abgehandelt werden sowie anderseits eine Fokussierung der Sozialarbeitenden auf der Elternebene festzustellen ist.
description: + Code Diss LU: hslusa masa be + Fussnote: Master of Science in Sozialer Arbeit, Bern, Luzern, St. Gallen, 2022 + NL-Code: NLLUHSA202209
Inhibition of the Calcium-dependent Tyrosine Kinase (CADTK) Blocks Monocyte Spreading and Motility
Creators: name: Morris, John S.
name: Golubovskaya, Vita
name: Harding, Timothy W.
name: Earp, H. Shelton
name: Haskill, J. Stephen
name: Hunter, Debra
name: Li, Xiong
name: Watson, Joanna M.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2001

description: Freshly isolated peripheral blood monocytes lack focal adhesion kinase (p125(FAK)) but activate a second member of this kinase family, calcium-dependent tyrosine kinase (CADTK; also known as Pyk2/CAKbeta/RAFTK/FAK2), upon adhesion or stimulation with chemokines. To study the role of CADTK in monocyte adherence and motility, we performed immunocytochemical localization that showed CADTK at the leading edge and ruffling lamellipodial structures in freshly isolated, adhered human monocytes. We next introduced CADTK/CAKbeta-related non-kinase (CRNK), the C-terminal noncatalytic domain of CADTK, into monocytes by electroporation and showed that it inhibited CADTK autophosphorylation. Introduction of the fusion protein glutathione S-transferase (GST)-CRNK also reduced (i) cell spreading, as reflected in a reduced cell area 30 min after adhesion, (ii) adhesion-induced phosphotyrosine increases and redistribution into lamellipodia, and (iii) adhesion-induced extracellular signal-regulated protein kinase (ERK) activation. In control experiments, introduction of GST or GST-C3 transferase (an inhibitor of RhoA GTPase activity) by electroporation did not affect these parameters. Monocytes adhered in the presence of autologous serum were highly motile even after introduction of GST (83% motile cells). However, only 26% of monocytes with introduced GST-CRNK were motile. In contrast, GST-CRNK-treated monocytes were fully capable of phagocytosis and adhesion-induced cytokine gene induction, suggesting that CADTK is not involved in these cellular activities and that GST-CRNK introduction does not inhibit global monocyte functions. These results suggest that CADTK is crucial for the in vitro monocyte cytoskeletal reorganization necessary for cell motility and is likely to be required in vivo for recruitment to sites of inflammation.
Large Binocular Telescope view of the atmosphere of GJ1214b
Creators: name: Nascimbeni, V.
name: Mallonn, M.
name: Scandariato, G.
name: Pagano, I.
name: Piotto, G.
name: Micela, G.
name: Messina, S.
name: Leto, G.
name: Strassmeier, K. G.
name: Bisogni, S.
name: Speziali, R.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Earth and Planetary Astrophysics (astro-ph.EP)
subject: Solar and Stellar Astrophysics (astro-ph.SR)
subject: FOS: Physical sciences

description: The atmospheric composition and vertical structure of the super-Earth GJ1214b has been a subject of debate since its discovery in 2009. Recent studies have indicated that high-altitude clouds might mask the lower layers. However, some data points that were gathered at different times and facilities do not fit this picture, probably because of a combination of stellar activity and systematic errors. We observed two transits of GJ1214b with the Large Binocular Camera, the dual-channel camera at the Large Binocular Telescope. For the first time, we simultaneously measured the relative planetary radius $k=R_\mathrm{p}/R_\star$ at blue and red optical wavelengths ($B+R$), thus constraining the Rayleigh scattering on GJ1214b after correcting for stellar activity effects. To the same purpose, a long-term photometric follow-up of the host star was carried out with WiFSIP at STELLA, revealing a rotational period that is significantly longer than previously reported. Our new unbiased estimates of $k$ yield a flat transmission spectrum extending to shorter wavelengths, thus confirming the cloudy atmosphere scenario for GJ1214b.
description: 11 pages, 5 figures, 3 tables. Published in A&A. Minor changes to reflect the published version
D1.5 Characterisation of national and regional data sources
Creators: name: University Of Parma
name: Aristotle University Of Thessaloniki
name: Akdeniz University
name: Cooperativas Agro-Alimentarias
name: Institute Of Agrophysics (IA PAS)
name: Idener
name: Bydgoszcz University Of Science And Technology
Publisher: name: Zenodo
Year: 2022
Subjects: AGRICORE H2020

description: This deliverable presents the methodology defined within the AGRICORE project to characterise national and regional data sources useful for performing agricultural research analysis. This methodology has been developed as part of the first of the work packages defined in the AGRICORE project. AGRICORE is a research project proposing an innovative way to apply agentbased modelling to improve the capacities of policymakers to evaluate the impact of agriculturalrelated measurements under and outside the framework of the Common Agricultural Policy. This project was funded by the European Commission as a result of the RUR-04-2018 call, part of the H2020 programme. In the introduction, the basis on which the methodology has been developed is presented, i.e., the ARDIT tool and the AGRICORE DCAT-AP 2.0 ontology. Then, the proposed methodology is detailed including also the definition of all the fields to be filled while characterising a dataset in ARDIT. As part of this methodology, also all the main challenges encountered during the characterisation of the datasets have been explained, illustrating the proposed solutions. After that, also the methodology proposed to guarantee that the tool ARDIT remains efficiently working is described, relying on a continuous upgrade and maintenance of the data. Finally, some conclusions regarding the characterisation process and the need for a governance strategy to ensure the survival of the ARDIT after the project are provided. It is important to remark that although this deliverable has been developed in the framework of the AGRICORE project, the participating partners have aimed for broader usage of the proposed methodology. As the final goal of this work package, the proposed EU Index Tool (now renamed as Agricultural Research Data Index Tool (ARDIT) aims to serve as a central entry point for locating useful datasets useful for agricultural research.
Identifying inter-thermal glides from Social eavesdropping allows for a more risky gliding strategy by thermal-soaring birds.
Creators: name: Williams, Hannah J.
name: King, Andrew J.
name: Duriez, Olivier
name: Börger, Luca
name: Shepard, Emily L. C.
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: Biological Engineering
subject: 110601 Biomechanics
subject: FOS: Health sciences
subject: 60104 Cell Metabolism
subject: FOS: Biological sciences
subject: 60199 Biochemistry and Cell Biology not elsewhere classified
subject: 69999 Biological Sciences not elsewhere classified

description: Glides were clearly identifiable as periods of straight flight and sustained altitude loss between bouts of circling behaviour (see Williams et al. 2015 for details). The circling is represented by a consistent sine-wave when the tri-axial magnetometry data is plotted against time. This indicates turning within the thermal updraft.
Alles so schön bunt hier !? – Konstruktionen der Adressat*innen durch Jugendarbeiter*innen der Offenen Jugendarbeit im Kanton Zürich: Eine intersektionale Analyse zu deren Bedeutung für Ein- und Ausschlüsse.
Creators: name: Vogel, Iris
Publisher: name: Zenodo
Year: 2022

description: Ausgangspunkt dieser Master-Thesis bildet die erste schweizweite Studie zu Einrichtungen der Offenen Jugendarbeit. Diese weist auf ein Spannungsfeld zwischen Anspruch und Wirklichkeit» (S. 73–78), bezüglich der tatsächlich Nutzenden und den konzeptionell definierten Zielgruppen hin. Ein intersektionaler Zugang zu besagten Spannungsfeld ermöglicht, Ein- und Ausschlüsse innerhalb kontextspezifischer Machtkonstellationen, sichtbar zu machen. Die vorliegende Master-Thesis erforscht, welche Wahrnehmungskategorien Professionelle der Offenen Jugendarbeit im Kanton Zürich gegenüber ihren Adressat*innen konstruieren. Zudem fragt sie, wie sich damit einhergehende Ein- und Ausschlussmechanismen aus einer intersektionalen Perspektive verordnen lassen. Die methodische Herangehensweise fusst auf einem qualitativen Forschungsparadigma, dass sich für die subjektive Eigenperspektive der Professionellen der Offenen Jugendarbeit interessiert. Dazu wurden vier halb-strukturierte leitfadengestützte Interviews durchgeführt. Die Interviews wurden anhand der Verfahrensweise der Grounded Theory ausgewertet und bilden den Ausgangspunkt der Ergebnisdiskussion aus einer intersektionalen heuristischen Analyseperspektive. Als Ergebnisse zeichnen sich vier Differenzkategorien ab (Mädchen*, Jugendliche mit Beeinträchtigungen; lesbische, schwule, bisexuelle, transsexuelle, transgender, intersexuelle und queere Jugendliche; Angebotsgruppen), welche von den Professionellen als Ein- beziehungsweise Ausschlusskategorien konstruiert werden. In den jeweiligen Differenzkategorien werden homogenisierende Zuschreibungen gemacht, sowie gesellschaftliche Diskurse und Stereotypisierungen bezüglich Ethnie, Geschlecht, Körper und Klasse, reproduziert. Dies verdeutlicht Verstrickungen der Professionellen im durchmachteten Raum der Offenen Jugendarbeit, in dem Zugehörigkeiten, Platzierungen und Schliessungsprozesse artikuliert und konstruiert werden. Um sich der eigenen Ausgrenzungsprozessen und Ausschliessungen bewusst zu werden, stellt die Selbstreflexivität der Professionellen der Offenen Jugendarbeit ein zentraler Aspekt dar. Notwendig dafür ist die professionelle Wahrnehmung und Reflexion der eigenen sozialen Positionierung in Machtverhältnissen wie auch der eigenen Vorurteile und Normativitätsvorstellungen.
description: + Code Diss LU: hslusa masa be + Fussnote: Master of Science in Sozialer Arbeit, Bern, Luzern, St. Gallen, 2022 + NL-Code: NLLUHSA202209
H$α$ and EUV observations of a partial CME
Creators: name: Christian, Damian J.
name: Jess, David B.
name: Antolin, Patrick
name: Mathioudakis, Mihalis
Publisher: name: arXiv
Year: 2015
Subjects: subject: Solar and Stellar Astrophysics (astro-ph.SR)
subject: FOS: Physical sciences

description: We have obtained H$α$ high spatial and time resolution observations of the upper solar chromosphere and supplemented these with multi-wavelength observations from the Solar Dynamic Observatory (SDO) and the {\it Hinode} ExtremeUltraviolet Imaging Spectrometer (EIS). The H$α$ observations were conducted on 11 February 2012 with the Hydrogen-Alpha Rapid Dynamics Camera (HARDcam) instrument at the National Solar Observatory's Dunn Solar Telescope. Our H$α$ observations found large downflows of chromospheric material returning from coronal heights following a failed prominence eruption. We have detected several large condensations ("blobs") returning to the solar surface at velocities of $\approx$200 km s$^{-1}$ in both H$α$ and several SDO AIA band passes. The average derived size of these "blobs" in H$α$ is 500 by 3000 km$^2$ in the directions perpendicular and parallel to the direction of travel, respectively. A comparison of our "blob" widths to those found from coronal rain, indicate there are additional smaller, unresolved "blobs" in agreement with previous studies and recent numerical simulations. Our observed velocities and decelerations of the "blobs" in both H$α$ and SDO bands are less than those expected for gravitational free-fall and imply additional magnetic or gas pressure impeding the flow. We derived a kinetic energy $\approx$2 orders of magnitude lower for the main eruption than a typical CME, which may explain its partial nature.
description: 31 pages, 8 Figures, 1 Table and 1 Video (linked to Figure 2). Accepted in ApJ
(1779) John Eliot
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
A Dynamical Model for the Formation of Gas Rings and Episodic Starbursts Near Galactic Centres
Creators: name: Krumholz, Mark R.
name: Kruijssen, J. M. Diederik
Publisher: name: arXiv
Year: 2015
Subjects: subject: Astrophysics of Galaxies (astro-ph.GA)
subject: FOS: Physical sciences

description: We develop a simple dynamical model for the evolution of gas in the centres of barred spiral galaxies, using the Milky Way's Central Molecular Zone (CMZ, i.e., the central few hundred pc) as a case study. We show that, in the presence of a galactic bar, gas in a disc in the central regions of a galaxy will be driven inwards by angular momentum transport induced by acoustic instabilities within the bar's inner Lindblad resonance. This transport process drives turbulence within the gas that temporarily keeps it strongly gravitationally stable and prevents the onset of rapid star formation. However, at some point the rotation curve must transition from approximately flat to approximately solid body, and the resulting reduction in shear reduces the transport rates and causes gas to build up, eventually producing a gravitationally-unstable region that is subject to rapid and violent star formation. For the observed rotation curve of the Milky Way, the accumulation happens $\sim 100$ pc from the centre of the Galaxy, in good agreement with the observed location of gas clouds and young star clusters in the CMZ. The characteristic timescale for gas accumulation and star formation is of order $10-20$ Myr. We argue that similar phenomena should be ubiquitous in other barred spiral galaxies.
description: 19 pages, 16 figures, MNRAS in press; this version has minor revisions to the discussion and added references, no other changes
The Star Cluster System in the Local Group Starburst Galaxy IC 10
Creators: name: Lim, Sungsoon
name: Lee, Myung Gyoon
Publisher: name: arXiv
Year: 2015
Subjects: subject: Astrophysics of Galaxies (astro-ph.GA)
subject: Cosmology and Nongalactic Astrophysics (astro-ph.CO)
subject: FOS: Physical sciences

description: We present a survey of star clusters in the halo of IC 10, a starburst galaxy in the Local Group based on Subaru R band images and NOAO Local Group Survey UBVRI images. We find five new star clusters. All these star clusters are located far from the center of IC 10, while previously known star clusters are mostly in the main body. Interestingly the distribution of these star clusters shows an asymmetrical structure elongated along the east and south-west direction. We derive UBVRI photometry of 66 star clusters including these new star clusters as well as previously known star clusters. Ages of the star clusters are estimated from the comparison of their UBVRI spectral energy distribution with the simple stellar population models. We find that the star clusters in the halo are all older than 1 Gyr, while those in the main body have various ages from very young (several Myr) to old (>1 Gyr). The young clusters (<10 Myr) are mostly located in the Hα emission regions and are concentrated on a small region at 2' in the south-east direction from the galaxy center, while the old clusters are distributed in a wider area than the disk. Intermediate-age clusters (~100 Myr) are found in two groups. One is close to the location of the young clusters and the other is at ~4' from the location of the young clusters. The latter may be related with past merger or tidal interaction.
description: 11 pages, 12 figures, 1 table; accepted for publication in ApJ
Inhibition of the Catalytic Activity of Cell Adhesion Kinase β by Protein-tyrosine Phosphatase-PEST-mediated Dephosphorylation
Creators: name: Schaefer, Erik M.
name: Lyons, Patrick D.
name: Dunty, Jill M.
name: Schaller, Michael D.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2001

description: Protein-tyrosine phosphatase (PTP)-PEST is a cytoplasmic tyrosine phosphatase that can bind and dephosphorylate the focal adhesion-associated proteins p130(CAS) and paxillin. Focal adhesion kinase (FAK) and cell adhesion kinase beta (CAKbeta)/PYK2/CADTK/RAFTK are protein-tyrosine kinases that can colocalize with, bind to, and induce tyrosine phosphorylation of p130(CAS) and paxillin. Thus, we considered the possibility that these kinases might be substrates for PTP-PEST. Using a combination of substrate-trapping assays and overexpression of PTP-PEST in mammalian cells, CAKbeta was found to be a substrate for PTP-PEST. Both the major autophosphorylation site of CAKbeta (Tyr(402)) and activation loop tyrosine residues, Tyr(579) and Tyr(580), were targeted for dephosphorylation by PTP-PEST. Dephosphorylation of CAKbeta by PTP-PEST dramatically inhibited CAKbeta kinase activity. In contrast, FAK was a poor substrate for PTP-PEST, and treatment with PTP-PEST had no effect on FAK kinase activity. Tyrosine phosphorylation of paxillin, which is greatly enhanced by CAKbeta overexpression, was dramatically reduced upon coexpression of PTP-PEST. Finally, endogenous PTP-PEST and endogenous CAKbeta were found to localize to similar cellular compartments in epithelial and smooth muscle cells. These results suggest that CAKbeta is a substrate of PTP-PEST and that FAK is a poor PTP-PEST substrate. Further, PTP-PEST can negatively regulate CAKbeta signaling by inhibiting the catalytic activity of the kinase.
Expectations and Experiences of Screen Time, Social Interaction, and Solitude
Creators: name: Wood, Natasha
name: Leckfor, Christina M.
name: Orehek, Edward
name: Kwiatek, Sarah
Publisher: name: Open Science Framework
Year: 2023
Subjects: subject: Social Psychology
subject: Social and Behavioral Sciences
subject: Psychology
subject: FOS: Psychology

description: In Study 1, undergraduate students forecasted their mood and loneliness when sitting alone, using their smartphone, and talking to a stranger. In Study 2, undergraduate students reported their experienced mood and loneliness after sitting alone, using their smartphone, and talking to a stranger. In Study 3, undergraduate students and U.S. adults forecasted their mood when sitting alone, texting, using social media, watching television, and talking to a stranger. In Study 4, undergraduate students reported their experienced mood after sitting alone, texting, using social media, watching television, and talking to a stranger.
Modification of Alternative Splicing of Bcl-x Pre-mRNA in Prostate and Breast Cancer Cells: ANALYSIS OF APOPTOSIS AND CELL DEATH
Creators: name: Kole, Ryszard
name: Cidlowski, John A.
name: Mercatante, Danielle R.
name: Bortner, Carl D.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2001

description: There is ample evidence that deregulation of apoptosis results in the development, progression, and/or maintenance of cancer. Since many apoptotic regulatory genes (e.g. bcl-x) code for alternatively spliced protein variants with opposing functions, the manipulation of alternative splicing presents a unique way of regulating the apoptotic response. Here we have targeted oligonucleotides antisense to the 5'-splice site of bcl-x(L), an anti-apoptotic gene that is overexpressed in various cancers, and shifted the splicing pattern of Bcl-x pre-mRNA from Bcl-x(L) to Bcl-x(S), a pro-apoptotic splice variant. This approach induced significant apoptosis in PC-3 prostate cancer cells. In contrast, the same oligonucleotide treatment elicited a much weaker apoptotic response in MCF-7 breast cancer cells. Moreover, although the shift in Bcl-x pre-mRNA splicing inhibited colony formation in both cell lines, this effect was much less pronounced in MCF-7 cells. These differences in responses to oligonucleotide treatment were analyzed in the context of expression of Bcl-x(L), Bcl-x(S), and Bcl-2 proteins. The results indicate that despite the presence of Bcl-x pre-mRNA in a number of cell types, the effects of modification of its splicing by antisense oligonucleotides vary depending on the expression profile of the treated cells.
(1780) Arabella Lucy Walsh
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
D1.6 Characterisation of datasets from previous research projects
Creators: name: University Of Parma
name: Akdeniz University
Publisher: name: Zenodo
Year: 2022
Subjects: AGRICORE H2020

description: This deliverable presents the methodology defined within the AGRICORE project to characterise datasets resulting from previous research that could be useful for performing agricultural research analysis in the future. This methodology has been developed as part of the first of the work packages defined in the AGRICORE project. AGRICORE is a research project proposing an innovative way to apply agent-based modelling to improve the capacities of policymakers to evaluate the impact of agricultural-related measurements under and outside the framework of the Common Agricultural Policy. This project was funded by the European Commission as a result of the RUR-04-2018 call, part of the H2020 programme. In the introduction, the basis on which the methodology has been developed is presented, i.e., the ARDIT tool and the AGRICORE DCAT-AP 2.0 ontology. Then, the proposed methodology is detailed including also the definition of all the fields to be filled while characterising a dataset in ARDIT. As part of this methodology, also all the main challenges encountered during the characterisation of the datasets have been explained, illustrating the proposed solutions. After that, also the methodology proposed to guarantee that the tool ARDIT remains efficiently working is described, relying on a continuous upgrade and maintenance of the data. Finally, some conclusions regarding the characterisation process and the need for a governance strategy to ensure the survival of the ARDIT after the project are provided. It is important to remark that although this deliverable has been developed in the framework of the AGRICORE project, the participating partners have aimed for broader usage of the proposed methodology. As the final goal of this work package, the proposed EU Index Tool (now renamed as Agricultural Research Data Index Tool (ARDIT) aims to serve as a central entry point for locating useful datasets useful for agricultural research.
Determination of the relative phase in ψ' and J/ψ decays into baryon and antibaryon
Creators: name: Zhu, K.
name: Mo, X. H.
name: Yuan, C. Z.
Publisher: name: arXiv
Year: 2015
Subjects: subject: High Energy Physics - Phenomenology (hep-ph)
subject: High Energy Physics - Experiment (hep-ex)
subject: FOS: Physical sciences

description: With the recent measurements of psi' and J/psi decay into octet-baryon pairs, we study the relative phase between the strong and the electromagnetic amplitudes, and find a large phase by fitting the present data. The fits take into account the details of experimental effects, including energy spread and initial state radiation. We also predict some branching fractions of psi' decays and the continuum production rates at the J/psi mass based on the relative phase and absolute amplitudes obtained from the fits.
description: 13 pages