Additional file 11 of Radiation-response in primary fibroblasts of long-term survivors of childhood cancer with and without second primary neoplasms: the KiKme study
Creators: name: Grandt, Caine Lucas
name: Brackmann, Lara Kim
name: Poplawski, Alicia
name: Schwarz, Heike
name: Hummel-Bartenschlager, Willempje
name: Hankeln, Thomas
name: Kraemer, Christiane
name: Marini, Federico
name: Zahnreich, Sebastian
name: Schmitt, Iris
name: Drees, Philipp
name: Mirsch, Johanna
name: Grabow, Desiree
name: Schmidberger, Heinz
name: Binder, Harald
name: Hess, Moritz
name: Galetzka, Danuta
name: Marron, Manuela
Publisher: name: figshare
Year: 2022
Subjects: subject: Biophysics
subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Physiology
subject: Ecology
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology

description: Additional file 11. Results of differential gene expression and pathway analyses stratified by sex. AF11a. Bar charts showing the number and proportion of up- and downregulated genes stratified by dose, donor group, and sex. Differentially expressed genes were computed comparing irradiated to sham-irradiated fibroblasts after exposure to 0.05 and 2 Gray (false discovery rate adjusted p-value &lt; 0.05), considering age at sampling. N0 = fibroblasts of cancer-free controls, N1 = fibroblasts of childhood-cancer survivors, N2+ = fibroblasts of childhood-cancer survivors with at least one second primary neoplasm; * p-value &lt; 0.05, ** p-value &lt; 0.01, *** p-value &lt; 0.001. AF11b. Upper Half: Volcano plots showing differential expression after 0.05 Gray stratified by donor group and sex, considering age at sampling. Lower Half: Heat map showing top five ranking genes with regard to p-value per group considering age at sampling. N0 = fibroblasts of cancer-free controls, N1 = fibroblasts of childhood-cancer survivors, N2+ = fibroblasts of childhood-cancer survivors with at least one second primary neoplasm; * p-value &lt; 0.05, ** p-value &lt; 0.01, *** p-value &lt; 0.001. N.S. = not significant (p-value &gt; 0.05). AF11c. Upper Half: Volcano plots showing differential expression after 2 Gray stratified by donor group and sex, considering age at sampling. Lower Half: Heat map showing top five ranking genes with regard to p-value per group considering age at sampling. N0 = fibroblasts of cancer-free controls, N1 = fibroblasts of childhood-cancer survivors, N2+ = fibroblasts of childhood-cancer survivors with at least one second primary neoplasm; * p-value &lt; 0.05, ** p-value &lt; 0.01, *** p-value &lt; 0.001. N.S. = not significant (p-value &gt; 0.05). AF11d. Overview of affected (false discovery rate adjusted p-value &lt; 0.05) and (in-) activated pathways (|z|≥ 2), predicted upstream effectors, downstream biofunctions and diseases, and observed molecular networks after irradiation with a low (0.05 Gray) or a high dose (2 Gray) ordered by p-value, stratified by sex and considering age at sampling. For molecular networks, the network score instead of a p-value and no z-score was calculated by Ingenuity Pathway Analysis. N0 = fibroblasts of cancer-free controls, N1 = fibroblasts of childhood-cancer survivors, N2+ = fibroblasts of childhood-cancer survivors with at least one second primary neoplasm; *p-value &lt; 0.05, ** p-value &lt; 0.01, ***p-value &lt; 0.001. AF11e. Heat map showing all pathways from Ingenuity Pathway Analysis that were significantly enriched in one of the three donor groups (false discovery rate adjusted p-value &lt; 0.05) using results from the differential gene expression data stratified by sex and considering age at sampling after exposure to 2 Gray. N0 = fibroblasts of cancer-free controls, N1 = fibroblasts of childhood-cancer survivors, N2+ = fibroblasts of childhood-cancer survivors with at least one second primary neoplasm; * p-value &lt; 0.05, ** p-value &lt; 0.01, *** p-value &lt; 0.001.