Endogenous Chemiluminescence from Germinating Arabidopsis Thaliana Seeds
Creators: name: Shafiekhani, Azizollah
name: Saeidfirozeh, Homa
name: Cifra, Michal
name: Masoudi, Amir Ali
Publisher: name: figshare
Year: 2020
Subjects: subject: 20301 Acoustics and Acoustical Devices; Waves
subject: FOS: Physical sciences
subject: FOS: Physical sciences
subject: Applied Physics
subject: Atomic Physics
subject: 20201 Atomic and Molecular Physics
subject: 20299 Atomic, Molecular, Nuclear, Particle and Plasma Physics not elsewhere classified
subject: 29901 Biological Physics
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: 20399 Classical Physics not elsewhere classified
subject: 20501 Classical and Physical Optics
subject: Computational Physics
subject: 20401 Condensed Matter Characterisation Technique Development
subject: 20402 Condensed Matter Imaging
subject: 20403 Condensed Matter Modelling and Density Functional Theory
subject: Condensed Matter Physics
subject: 20601 Degenerate Quantum Gases and Atom Optics
subject: 20404 Electronic and Magnetic Properties of Condensed Matter; Superconductivity
subject: 20302 Electrostatics and Electrodynamics
subject: General Relativity
subject: 20502 Lasers and Quantum Electronics
subject: Mechanics
subject: 29903 Medical Physics
subject: 20202 Nuclear Physics
subject: 20599 Optical Physics not elsewhere classified
subject: 20203 Particle Physics
subject: 20504 Photonics, Optoelectronics and Optical Communications
subject: 29999 Physical Sciences not elsewhere classified
subject: 20405 Soft Condensed Matter
subject: Special Relativity
subject: 20406 Surfaces and Structural Properties of Condensed Matter
subject: 29904 Synchrotrons; Accelerators; Instruments and Techniques
subject: Thermodynamics
subject: 20304 Thermodynamics and Statistical Physics
subject: FOS: Mechanical engineering
subject: FOS: Mechanical engineering

description: It is well known that all biological systems which undergo oxidative metabolism or oxidative stress generate a small amount of light. Since the origin of excited states producing this light is generally accepted to come from chemical reactions, the term endogenous biological chemiluminescence is appropriate. Apart from biomedicine, this phenomenon has potential applications also in plant biology and agriculture like monitoring the germination rate of seeds. While chemiluminescence capability to monitor germination has been measured on multiple agriculturally relevant plants, the standard model plant Arabidopsis thaliana has not been analyzed for this process so far. To fill in this gap, we demonstrate here on A. thaliana that the intensity of endogenous chemiluminescence increases during the germination stage. We showed that the chemiluminescence intensity increases since the second day of germination, but reaches a plateau on the third day, in contrast to other plants germinating from larger seeds studied so far. We also showed that intensity increases after topical application of hydrogen peroxide in a dose-dependent manner. Further, we demonstrated that the entropy of the chemiluminescence time series is similar to random Poisson signals. Our results support a notion that metabolism and oxidative reactions are underlying processes which generate endogenous biological chemiluminescence. Our findings contribute to novel methods for non-invasive and label-free sensing of oxidative processes in plant biology and agriculture. <br>