Additional file 1 of Clinical and immunological outcomes of HIV-exposed uninfected and HIV-unexposed uninfected children in the first 24 months of life in Western Kenya
Creators: name: Ray, Jessica E.
name: Dobbs, Katherine R.
name: Ogolla, Sidney O.
name: Daud, Ibrahim I.
name: Midem, David
name: Omenda, Maxwel M.
name: Nowacki, Amy S.
name: Beeson, James G.
name: Sabourin, Katherine R.
name: Rochford, Rosemary
name: Dent, Arlene E.
Publisher: name: figshare
Year: 2024
Subjects: subject: Immunology
subject: FOS: Clinical medicine

description: Additional file 1: Supplementary Table 1. Sources of recombinant P. falciparum protein antigens, protein concentrations used in conjugation to magnetic microspheres, expression systems, and salient references. Supplementary Table 2. Birth characteristics of HEU and HUU infants. Mean gestational age, birth weight, length, and head circumference were compared between the two groups using the Student’s t-test; sd, standard deviation. Supplementary Table 3. Linear growth curve models of Z scores for weight, length, head circumference, and BMI for age in HEU vs. HUU children, obtained using Bayesian hierarchical regression. HDI, highest probability density interval; sd, standard deviation. Region of practical equivalence (ROPE) for contrast terms for intercepts and slopes set at -0.05, 0.05. Supplementary Table 4. Fixed effects regression models for plasma cytokine levels. Twelve cytokines were measured at 8 visits (birth, 6, 10, 14, 18, 26, 39, and 52 weeks of age). A total of 663 visits were analyzed. Differences between HEU and HUU children in plasma cytokine trajectories were tested by evaluating the group*time interaction effect. If there was no difference, we tested whether HEU values differed from HUU values (group main effect) and whether values changed over time within a group (time main effect) (of interest was the 26 weeks vs. birth comparison, which is included in the table). Supplementary Table 5. Fixed effects regression models for vaccine-specific antibody levels. Antibodies against four vaccines were measured at 12 visits (birth, 6, 10, 14, 18 weeks, 6, 9, 12, 15, 18, 21, and 24 months of age). A total of 782 visits were analyzed. Differences between HEU and HUU children in vaccine antibody trajectories were tested by evaluating the group*time interaction effect. We then tested whether HEU values differed from HUU values (group main effect) and whether values changed over time within a group (time main effect) (of interest were the 6 months vs. birth and 18 months vs. 6 months comparisons, which are included in the table). Supplementary Table 6. Fixed effects regression models for antimalarial antibody levels (interaction effects and main effects for group). Antibodies against 14 P. falciparum antigens were measured at 12 visits (birth, 6, 10, 14, 18 weeks, 6, 9, 12, 15, 18, 21, and 24 months of age). A total of 1,135 visits were analyzed. Differences between HEU and HUU children in antimalarial antibody trajectories were tested by evaluating group*time interaction effect. For 12 of 14 antigens, we then tested whether HEU values differed from HUU values (group main effect); mean antibody values (fold over North American controls) of the group averaged over all time points are listed. Supplementary Table 7. Fixed effects regression models for antimalarial antibody levels (main effects for time). Antibodies against 14 P. falciparum antigens were measured at 12 visits (birth, 6, 10, 14, 18 weeks, 6, 9, 12, 15, 18, 21, and 24 months of age). A total of 1,135 visits were analyzed. For 12 of 14 antigens, we tested whether values changed over time independent of group. Of interest were 6 months vs. birth and 18 months vs. 6 months comparisons. Mean antibody values (fold over North American controls) at the three time points are listed.