cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Predicting Individuals’ Affective Well-Being by Applying Natural Language Processing to Daily Diaries
Creators: name: Scharbert, Julian
name: Klinz, Johannes Leonhard
name: Back, Mitja
Publisher: name: Open Science Framework
Year: 2023
Subjects: subject: Personality and Social Contexts
subject: Social Psychology
subject: Psychology
subject: FOS: Psychology
subject: Social and Behavioral Sciences
subject: Language
subject: Well-Being
subject: Machine Learning
subject: Multilevel Modeling
subject: Natural Language Processing

description: Psychological researchers assume that our language behavior and affective well-being are closely linked. There are already many studies testing this assumption in between-subjects designs, especially in the social media context - a handful of studies also tested this assumption in within-subjects designs. However, the existing knowledge is limited to (1) mainly correlational designs, (2) the consideration of very particular language features (i.e., no combination of content and paraverbal cues), and (3) models that do not combine multilevel and machine learning methodology. Our project aims at a comprehensive linguistic multilevel machine learning approach to predict affective well-being in everyday life. Specifically, two questions will be tackled: (1) How much are the developments of language behavior and affective well-being associated over time (co-development)? (2) How do language behavior and well-being affect each other over time (mutual influence)?
(2032) Samuel Wood
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Rhodopsin mutants discriminate sites important for the activation of rhodopsin kinase and Gt
Creators: name: Osawa, Shoji
name: Shi, Wen
name: Weiss, Ellen R.
name: Dickerson, Charlene D.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: The cytoplasmic loops of rhodopsin, the rod cell photoreceptor, play important regulatory roles in the activation of both rhodopsin kinase and the rod cell G protein, Gt. A number of studies have identified domains in rhodopsin that are important for the activation of Gt. However, less is known concerning the cytoplasmic regions that regulate phosphorylation of the photoreceptor by rhodopsin kinase. To identify regions that participate in these processes, a series of alanine mutations were generated in the three cytoplasmic loops of rhodopsin and transiently expressed in HEK-293 cells. Membranes prepared from these cells were reconstituted with the opsin chromophore, 11-cis-retinal, and characterized for their ability to undergo light-dependent phosphorylation by rhodopsin kinase and to catalyze GTP gamma S (guanosine 5'-O-(3-thiotriphosphate)) binding to Gt. We have identified mutants that fall into three distinct categories: 1) those that show altered phosphorylation but normal Gt activation, such as T62A/V63A/Q64A and R147A/F148A/G149A in Loops I and II, respectively; 2) mutants that have reduced ability to activate Gt but are phosphorylated normally, including T242A/T243A and V250A/T251A/R252A in Loop III; and 3) mutants that affect both phosphorylation and Gt activation, including A233G/A234G/A235G and A233N/A234N/A235N in Loop III. The use of these two assays in parallel have allowed us to distinguish the presence of distinct functional domains within the cytoplasmic loops which are specific for interaction with rhodopsin kinase or Gt.
$\mathcal{N}=1$ Curves on Generalized Coulomb Branches
Creators: name: Bourton, Thomas
name: Pomoni, Elli
name: Zhang, Xinyu
Publisher: name: arXiv
Year: 2021
Subjects: subject: High Energy Physics - Theory (hep-th)
subject: FOS: Physical sciences

description: We study the low energy effective dynamics of four-dimensional $\mathcal{N}=1$ supersymmetric gauge theories of class $\mathcal{S}_k$ on the generalized Coulomb branch. The low energy effective gauge couplings are naturally encoded in algebraic curves $\mathcal{X}$, which we derive for general values of the couplings and mass deformations. We then recast these IR curves $\mathcal{X}$ to the UV or M-theory form $\mathcal{C}$: the punctured Riemann surfaces on which the six-dimensional $\mathcal{N}=(1,0)$ ${A_{k-1}}$ SCFTs are compactified giving the class $\mathcal{S}_k$ theories. We find that the UV curves $\mathcal{C}$ and their corresponding meromorphic differentials take the same form as those for their mother four-dimensional $\mathcal{N}=2$ theories of class $\mathcal{S}$. They have the same poles, and their residues are functions of all the exactly marginal couplings and the bare mass parameters which we can compute exactly.
description: 22 pages; to be published in Universe
The Effect of Carboxyl-terminal Mutagenesis of G on Rhodopsin and Guanine Nucleotide Binding
Creators: name: Osawa, Shoji
name: Weiss, Ellen R.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: The carboxyl terminus of G protein alpha subunits plays an important role in receptor recognition. To identify the amino acids that participate in this interaction, COOH-terminal mutants of alpha t (the transducin alpha subunit) were expressed in vitro and analyzed for their ability to interact with rhodopsin and to bind guanine nucleotide. Gly-348, the reported site of a beta turn, was replaced with other neutral amino acids without severely affecting rhodopsin binding. However, proline substitution abolished rhodopsin interaction, suggesting that flexibility is important at this site. A comparison between C347Y, which lost both rhodopsin and guanine nucleotide binding, and a mutant substituted with alpha q sequence (D346E/C347Y/G348N/F350V), in which guanine nucleotide binding was restored, implies that distinct motifs maintain the structure of the alpha subunit and are necessary for selective interaction with receptors. Surprisingly, mutants L344A, L349A, F350stop, and stop351A demonstrated a parallel loss of rhodopsin and guanine nucleotide binding. Altered profiles of L344A and F350stop on sucrose density gradients indicate that these mutants may undergo denaturation. The equivalent of alpha tL344A generated in alpha s and alpha i did not show such a severe loss of guanine nucleotide binding, revealing that the alpha t carboxyl terminus is unique in its susceptibility to changes in amino acid sequence.
Additional file 2 of Plasma Neuron-Specific Enolase is not a reliable biomarker for staging Trypanosoma brucei rhodesiense sleeping sickness patients
Creators: name: Kato, Charles D.
name: Twesigye, Dorothy
name: Alibu, Vincent P.
name: Nanteza, Ann
name: Nsubuga, Julius
name: Mugasa, Claire M.
name: Matovu, Enock
Publisher: name: figshare
Year: 2022
Subjects: subject: Space Science
subject: Cell Biology
subject: Immunology
subject: FOS: Clinical medicine
subject: Developmental Biology

description: Additional file 2: Figure S2. Power calculation for cases and controls.
Simulation input conditions from Comment on ‘The cutting of metals by plastic buckling’ by Udupa et al.
Creators: name: T. H. C. Childs
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: Mechanical Engineering
subject: FOS: Mechanical engineering
subject: Mechanics
subject: 30399 Macromolecular and Materials Chemistry not elsewhere classified
subject: FOS: Chemical sciences

description: Text and a listing of all geometry, material, process and meshing input data for the simulations
(2034) Amos Wood
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
The General Transcription-Repair Factor TFIIH Is Recruited to the Excision Repair Complex by the XPA Protein Independent of the TFIIE Transcription Factor
Creators: name: Reardon, J. T.
name: Park, C. H.
name: Sancar, A.
name: Mu, D.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: Recent studies have revealed that the general transcription factor TFIIH is also a general excision repair factor which, along with several other proteins, is required for transcription-independent excision reaction. As a general transcription factor, TFIIH is recruited to RNA polymerase II-promoter complex by another general transcription factor called TFIIE. We were interested in knowing whether TFIIE is also involved in recruiting TFIIH to the excision repair complex. We found that cell-free extract depleted of TFIIE carried out excision repair at a normal rate, leading us to conclude that TFIIE is not involved in recruiting TFIIH to the damage site and has no role in general excision repair. In contrast, the human damage recognition protein XPA specifically binds to TFIIH and apparently recruits it to the damage site. The carboxyl-terminal half of XPA is responsible for specific interaction with TFIIH. The C261S/C264S mutant of XPA bound the ERCC1-XPF complex normally, but failed to bind TFIIH and failed to complement an XP-A mutant cell-free extract indicating that the XPA-TFIIH interaction is essential to effecting the excision reaction. Interestingly, XPA also binds to the p34 subunit of TFIIE specifically and in competition with the p56 subunit of TFIIE. This latter interaction has no apparent role in general excision repair but may be relevant in the transcription-coupled repair reaction.
The Transcriptional Regulatory Protein, YB-1, Promotes Single-stranded Regions in the DRA Promoter
Creators: name: MacDonald, G. H.
name: Ting, J. P.Y.
name: Itoh-Lindstrom, Y.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: YB-1 is a member of a newly defined family of DNA- and RNA-binding proteins, the Y box factors. These proteins have been shown to affect gene expression at both the transcriptional and translational levels. Recently, we showed that YB-1 represses interferon-gamma-induced transcription of class II human major histocompatibility (MHC) genes (1). Studies in this report characterize the DNA binding properties of purified, recombinant YB-1 on the MHC class II DRA promoter. The generation of YB-1-specific antibodies further permitted an analysis of the DNA binding properties of endogenous YB-1. YB-1 specifically binds single-stranded templates of the DRA promoter with greater affinity than double-stranded templates. The single-stranded DNA binding sites of YB-1 were mapped to the X box, whereas the double-stranded binding sites were mapped to the Y box of the DRA promoter, by methylation interference analysis. Most significantly, YB-1 can induce or stabilize single-stranded regions in the X and Y elements of the DRA promoter, as revealed by mung bean nuclease analysis. In concert with the findings that YB-1 represses DRA transcription, this study of YB-1 binding properties suggests a model of repression in which YB-1 binding results in single-stranded regions within the promoter, thus preventing loading and/or function of other DRA-specific transactivating factors.
Transcription Factor AP-2 Regulates Human Insulin-like Growth Factor Binding Protein-5 Gene Expression
Creators: name: Duan, C.
name: Clemmons, D. R.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: Insulin-like growth factor binding protein-5 (IG-FBP-5) is an important modulator of IGF actions. IG-FBP-5 mRNA is abundant in human fibroblasts and is regulated by cAMP. To understand the molecular mechanism underlying this cell type-specific expression and regulation, we isolated the 5'-flanking region of the human IGFBP-5 gene and fused it to a promoter-less reporter plasmid encoding luciferase. Transient transfection of the construct into fibroblasts displayed both constitutive and cAMP-induced promoter activity in an orientation-specific manner. Sequence analysis revealed the existence of distal and proximal consensus AP-2 recognition sites located 5' from the TATA box. Both sequences bound specifically to human AP-2 in vitro by gel shift mobility assay. The possible role of AP-2 was examined by cotransfection of AP-2-deficient HepG2 cells with the IGFBP-5 promoter construct and a human AP-2 expression construct. Cotransfection with AP-2 significantly elevated IGFBP-5 promoter activity. This trans-activation was IGFBP-5 promoter and AP-2 specific. In AP-2 abundant fibroblasts, expression of AP-2B, a dominant-negative inhibitor of AP-2, suppressed IGFBP-5 promoter activity. In HepG2 cells, AP-2B alone had no significant effect, but the AP-2-induced activation of promoter activity was inhibited by AP-2B in a dose-dependent manner. The relative functional importance of the putative AP-2 binding sites was examined using a number of deletion mutants and point mutations. When the first two distal CCCCACCC-like putative AP-2 sites were deleted or mutated, there was no change in AP-2-induced trans-activation. Deletion or mutation of the proximal GCCNNNGGC-like sequences, however, abolished the AP-2-induced activation. These results suggest that AP-2 regulates the IGFBP-5 gene expression through the proximal GCCNNNGGC-like sequences. This AP-2-mediated trans-activation contributes at least in part to the constitutively high expression of IGFBP-5 in fibroblasts and to the cAMP responsiveness of this gene.
Text S1: Details on Robofish design and features from Using a robotic fish to investigate individual differences in social responsiveness in the guppy.
Creators: name: Bierbach, David
name: Landgraf, Tim
name: Romanczuk, Pawel
name: Lukas, Juliane
name: Nguyen, Hai
name: Wolf, Max
name: Krause, Jens
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: 170299 Cognitive Science not elsewhere classified
subject: FOS: Psychology
subject: 90301 Biomaterials
subject: FOS: Medical engineering
subject: 90602 Control Systems, Robotics and Automation
subject: FOS: Electrical engineering, electronic engineering, information engineering
subject: 30402 Biomolecular Modelling and Design
subject: FOS: Chemical sciences

description: Responding towards the actions of others is one of the most important behavioural traits whenever animals of the same species interact. Mutual influences among interacting individuals may modulate the social responsiveness seen and thus make it often difficult to study the level and individual variation in responsiveness. Here, open-loop biomimetic robots that provide standardized, non-interactive social cues can be a useful tool. These robots are not affected by the live animal's actions but are assumed to still represent valuable and biologically relevant social cues. As this assumption is crucial for the use of biomimetic robots in behavioural studies, we hypothesized (i) that meaningful social interactions can be assumed if live animals maintain individual differences in responsiveness when interacting with both a biomimetic robot and a live partner. Furthermore, to study the level of individual variation in social responsiveness, we hypothesized (ii) that individual differences should be maintained over the course of multiple tests with the robot. We investigated the response of live guppies (Poecilia reticulata) when allowed to interact either with a biomimetic open-loop-controlled fish robot—‘Robofish’—or with a live companion. Furthermore, we investigated the responses of live guppies when tested three times with Robofish. We found that responses of live guppies towards Robofish were weaker compared with those of a live companion, most likely as a result of the non-interactive open-loop behaviour of Robofish. Guppies, however, were consistent in their individual responses between a live companion and Robofish, and similar individual differences in response towards Robofish were maintained over repeated testing even though habituation to the test environment was detectable. Biomimetic robots like Robofish are therefore a useful tool for the study of social responsiveness in guppies and possibly other small fish species.
(2030) Helen [Wood]
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Two Distinct Raf Domains Mediate Interaction with Ras
Creators: name: Drugan, Jonelle K.
name: Ghosh, Sujoy
name: Der, Channing J.
name: Campbell-Burk, Sharon
name: Bell, Robert M.
name: Terrell, Regina S.
name: Brtva, Teresa R.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: A key event for Ras transformation involves the direct physical association between Ras and the Raf-1 kinase. This interaction promotes both Raf translocation to the plasma membrane and activation of Raf kinase activity. Although substantial experimental evidence has demonstrated that Raf residues 51-131 alone are sufficient for Ras binding, conflicting observations have suggested that the Raf cysteine-rich domain (residues 139-184) may also be important for interaction with Ras. To clarify the role of the Raf cysteine-rich domain in Ras-Raf binding, we have compared the ability of two distinct Raf fragments to interact with Ras using both in vitro Ras binding and in vivo Ras inhibition assays. First, we determined that both Raf sequences 2-140 and 139-186 (designated Raf-Cys) showed preferential binding to active, GTP-bound Ras in vitro. Second, we observed that Raf-Cys antagonized oncogenic Ras(Q61L)-mediated transactivation of Ras-responsive elements and focus-forming activity in NIH 3T3 cells and insulin-induced germinal vesicle breakdown in Xenopus laevis oocytes in vivo. This inhibitory activity suggests that Raf-Cys can interact with Ras in vivo. Taken together, these results suggest that Ras interaction with two distinct domains of Raf-1 may be important in Ras-mediated activation of Raf kinase activity.
Dibujos: Edzná (Campeche, México) - documentación en 3D del Edificio de los Cinco Pisos
Creators: name: Campoverde, Alejandro
name: Paap, Iken
name: Jansen, Philipp
Publisher: name: Zenodo
Year: 2021
Subjects: arqueología maya
arquitectura maya
Campeche
Mexico

description: Dibujos: Edzná (Campeche, México) - documentación en 3D del Edificio de los Cinco Pisos
 (Alejandro Campoverde, Iken Paap, Philipp Sebastian Jansen, 2021)
 https://doi.org/10.5281/zenodo.4624899
 Coordinación: Iken Paap (IAI Berlin), Jan Pieper (RWTH Aachen), Antonio Benavides C. (INAH Campeche)
 Levantamiento: Philipp S. Jansen (Berlin), Alejandro Campoverde (Aachen)
 Procesamiento: Philipp S. Jansen (Berlin), Alejandro Campoverde (Aachen)
 Dibujo: Alejandro Campoverde (Aachen)
 Escalera jeroglífica: Sven Gronemeyer (Bonn), Karl-Herbert Mayer (Graz)
 Financiamiento:
 • Levantamiento por escaneo 3D, procesamiento y dibujo: Ibero-Amerikanisches Institut – Stiftung Preußischer Kultubesitz (IAI-SPK)
 • Levantamiento por SfM: Deutsche Forschungsgemeinschaft (DFG), en el marco del proyecto “Architektur und Konstruktion textiler Verdachungen im Steinbau der Maya“ (RWTH Aachen)
 Contacto: paap@iai.spk-berlin.de
Additional file 3 of Plasma Neuron-Specific Enolase is not a reliable biomarker for staging Trypanosoma brucei rhodesiense sleeping sickness patients
Creators: name: Kato, Charles D.
name: Twesigye, Dorothy
name: Alibu, Vincent P.
name: Nanteza, Ann
name: Nsubuga, Julius
name: Mugasa, Claire M.
name: Matovu, Enock
Publisher: name: figshare
Year: 2022
Subjects: subject: Space Science
subject: Cell Biology
subject: Immunology
subject: FOS: Clinical medicine
subject: Developmental Biology

description: Additional file 3: Figure S3. Standard curve for NSE.
A Conserved Domain of the viviparous-1 Gene Product Enhances the DNA Binding Activity of the bZIP Protein EmBP-1 and Other Transcription Factors
Creators: name: Hill, Alison
name: Nantel, André
name: Rock, Christopher D.
name: Quatrano, Ralph S.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: The maize VP1 protein is a seed-specific regulator of gene expression that effects the expression of a subset of abscisic acid (ABA)-regulated genes that are expressed during the maturation program of the seed. In addition, VP1 has pleiotropic effects on seed development that are not related to ABA. In transient expression assays, VP1 has been shown to transactivate gene expression through at least two distinct promoter elements: the G boxes from the ABA-inducible wheat Em gene and the SphI box from the maize C1 gene. We have investigated how VP1 can transactivate gene expression through diverse promoter elements by analyzing its association in vitro with EmBP-1, a factor that binds the Em promoter. We demonstrate that VP1 can greatly enhance the DNA binding activity of EmBP-1 in a gel retardation assay. This enhancing activity has also been observed on transcription factors as diverse as Opaque-2, Max, Sp1, and NF-kappaB. Deletion of a small but highly conserved region (BR2) in VP1 eliminates the enhancement in vitro as well as the ability of VP1 to transactivate Em gene expression in a transient expression assay. A 40-amino acid fragment from VP1 sandwiched between the maltose-binding protein and LacZ can confer the enhancement function to this fusion protein in vitro. A weak and relatively nonspecific interaction between BR2 and DNA is demonstrated by UV cross-linking. The in vitro properties we observe for VP1 might explain the regulatory effects of VP1 on a diverse set of genes and why mutations in the vp1 locus have pleiotropic effects.
(2026) John Thomas Sipling
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
A Conserved HPD Sequence of the J-domain Is Necessary for YDJ1 Stimulation of Hsp70 ATPase Activity at a Site Distinct from Substrate Binding
Creators: name: Douglas, Michael G.
name: Tsai, Joyce
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: The 46-kDa protein YDJ1 is one of several known yeast homologues of the Escherichia coli DnaJ protein. Like all J homologues, it shares homology with the highly conserved NH2-terminal "J-domain" of DnaJ. A component of the DnaK (Hsp70) chaperone machinery that mediates protein folding, DnaJ is necessary for survival at elevated temperatures. It stimulates ATP hydrolysis by DnaK and effects the release of DnaK-bound polypeptides. Previous genetic and biochemical studies indicate that the J-domain is necessary for these functions. Using peptides corresponding to J-domain sequence, we show that a peptide containing the highly conserved His-Pro-Asp sequence at positions 34-36 in the J-domain competes off YDJ1 stimulation of Hsp70 ATPase activity. Inhibitory concentrations of peptide do not prevent binding of folding substrates, therefore YDJ1 must interact with Hsp70 at a site distinct from that for substrate binding. This interaction is critical for Hsp70 activity, since a mutant YDJ1 protein harboring a H34Q change (ydj1Q34) stimulates neither Hsp70 ATPase nor substrate release. The importance of the proper function of this region of the protein is supported by the poor growth and temperature-sensitive phenotype of yeast expressing ydj1Q34.
A Guanine Nucleotide-independent Inwardly Rectifying Cation Permeability Is Associated with P2Y 1 Receptor Expression in Xenopus Oocytes
Creators: name: Harden, T. Kendall
name: Elmquist, Eric
name: O'Grady, Scott M.
name: Filtz, Theresa M.
name: Nicholas, Robert A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: The functional properties of the G protein-coupled P2Y1 receptor were investigated in Xenopus oocytes. Incubation of oocytes expressing either the human or turkey P2Y1 receptor with adenine nucleotide agonists resulted in an increase in Cl- current and activation of a novel cation current with an inwardly rectifying current-voltage relationship. Activation of either the human P2Y2 (P2U-purinergic) or M1 muscarinic receptor expressed in oocytes resulted in an increase in Cl- current similar to that observed in P2Y1 receptor-expressing oocytes but had no effect on cation current. P2 receptor agonists stimulated both the cation current and Cl- current in P2Y1 receptor-expressing oocytes with EC50 values and an order of potency (2-methylthioadenosine diphosphate > 2-methylthioadenosine triphosphate (2MeSATP) > ATP > UTP) that were similar to those previously observed for activation of phospholipase C in 1321N1 human astrocytoma cells stably expressing the human or turkey P2Y1 receptor. The P2Y receptor antagonists suramin and pyridoxal phosphate 6-azophenyl-2'-4'-disulfonic acid both shifted to the right the concentration-response relationship for 2MeSATP for stimulation of oocyte currents. Although injection of oocytes with either GDPbetaS (guanyl-5'-yl thiophosphate) or GTPgammaS (guanosine 5'-3-O-(thio)triphosphate) resulted in loss of adenine nucleotide-promoted Cl- channel activation, neither guanine nucleotide altered the 2MeSATP-stimulated cation current. These data are consistent with the view that activation of the novel cation current by the P2Y1 receptor does not involve a G protein. Tail current analysis of the novel P2Y1 receptor-associated cation conductance revealed that the open channel current-voltage relationship was outwardly rectifying with a reversal potential of -38 mV for the turkey P2Y1 receptor and -36 mV for the human P2Y1 receptor. Replacement of Na+ with K+ ions in the bathing solution produced a shift in reversal potential to near zero mV, but significant outward rectification remained. The cation current was not permeable to either Ca2+ or Ba2+ and exhibited steady-state inactivation at holding potentials below -60 mV. These results indicate that the P2Y1 receptor exhibits both metabotropic properties and a novel G protein-independent ionotropic response when expressed in Xenopus oocytes.
Barjols, hôtel du Pont d'or, Maguy Pichonnet-Andral enregistre les réponses du félibre Gabriel Larose, fondateur de l'Escolo Caramy de Brignoles, aux questions de Claudie Marcel-Dubois
Creators: name: Musée National Des Arts Et Traditions Populaires (Paris)
name: Centre D'ethnologie Française
Publisher: name: École des Hautes Études en Sciences Sociales; Institut  interdisciplinaire d'anthropologie du contemporain (Paris); Laboratoire d'anthropologie et d'histoire de l'institution de la culture (Paris)
Year: 2024

Abstract
A Partially Functional DNA Helicase II Mutant Defective in Forming Stable Binary Complexes with ATP and DNA: A ROLE FOR HELICASE MOTIF III
Creators: name: Matson, Steven W.
name: Brosh Jr., Robert M.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: To address the functional significance of motif III in Escherichia coli DNA helicase II, the conserved aspartic acid at position 248 was changed to asparagine. UvrDD248N failed to form stable binary complexes with either DNA or ATP. However, UvrDD248N was capable of forming an active ternary complex when both ATP and single-stranded DNA were present. The DNA-stimulated ATPase activity of UvrDD248N was reduced relative to that of wild-type UvrD with no significant change in the apparent Km for ATP. The mutant protein also demonstrated a reduced DNA unwinding activity. The requirement for high concentrations of UvrDD248N to achieve unwinding of long duplex substrates likely reflects the reduced stability of various binary and ternary complexes that must exist in the catalytic cycle of a helicase. The data suggest that motif III may act as an interface between the ATP binding and DNA binding domains of a helicase. The uvrDD248N allele was also characterized in genetic assays. The D248N protein complemented the UV-sensitive phenotype of a uvrD deletion strain to levels nearly equivalent to wild-type helicase II. In contrast, the mutant protein only partially complemented the mutator phenotype. A correlation between the level of genetic complementation and the helicase activity of UvrDD248N is discussed.
The role of interpersonal problems in the treatment of posttraumatic stress disorder
Creators: name: Wolf, Lisa
name: Dr. Larissa Wolkenstein
Publisher: name: Open Science Framework
Year: 2023
Subjects: subject: Psychological Phenomena and Processes
subject: Medicine and Health Sciences
subject: Social and Behavioral Sciences
subject: Psychiatry and Psychology
subject: Clinical Psychology
subject: Psychology
subject: FOS: Psychology
subject: Mental and Social Health
subject: Cognitive Behavioral Therapy
subject: interpersonal problems
subject: interpersonal trauma
subject: trauma therapy
subject: PTSD
subject: interpersonal circumplex

description: This is a Master's thesis project at LMU Munich and the outpatient clinic of LMU.
Mobilizing the Racial In-Between: The Impact of Discrimination on Asian American Coalition Building October 4 2022 Update
Creators: name: Chan, Stephanie
Publisher: name: Open Science Framework
Year: 2023
Subjects: subject: Political Science
subject: FOS: Political science
subject: Social and Behavioral Sciences
subject: Chan, Stephanie, and Sonya Chen. 2022. “Mobilizing the Racial In-between: The Impact of Discrimination on Asian American Coalition Building.” OSF. September 23. osf.io/rt9za.

description: Before collecting data, we have modified our plans for collecting the data as well as the survey instrument. These changes were made in response to constructive suggestions from colleagues. We detail the changes we have made to the pre-registration for "Mobilizing the Racial In-Between: The Impact of Discrimination on Asian American Coalition Building" (Chan, Stephanie, and Sonya Chen. 2022. “Mobilizing the Racial In-between: The Impact of Discrimination on Asian American Coalition Building.” OSF. September 23. osf.io/rt9za.) in this pre-registration.
Activation of a Novel Calcium-dependent Protein-tyrosine Kinase: CORRELATION WITH c-Jun N-TERMINAL KINASE BUT NOT MITOGEN-ACTIVATED PROTEIN KINASE ACTIVATION
Creators: name: Graves, Lee M.
name: Marchetto, Gail S.
name: Calvo, Benjamin
name: Hunter, Deborah
name: Shelton Earp, H.
name: Li, Xiong
name: Anderegg, Robert J.
name: Wilm, Matthias
name: Yu, Hong
name: Dawson, Tom L.
name: Dy, Ruth
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: Many G protein-coupled receptors (e.g. that of angiotensin II) activate phospholipase Cbeta, initially increasing intracellular calcium and activating protein kinase C. In the WB and GN4 rat liver epithelial cell lines, agonist-induced calcium signals also stimulate tyrosine phosphorylation and subsequently increase the activity of c-Jun N-terminal kinase (JNK). We have now purified the major calcium-dependent tyrosine kinase (CADTK), and by peptide and nucleic acid sequencing identified it as a rat homologue of human PYK2. CADTK/PYK2 is most closely related to p125(FAK) and both enzymes are expressed in WB and GN4 cells. Angiotensin II, which only slightly increases p125(FAK) tyrosine phosphorylation in GN4 cells, substantially increased CADTK tyrosine autophosphorylation and kinase activity. Agonists for other G protein-coupled receptors (e.g. LPA), or those increasing intracellular calcium (thapsigargin), also stimulated CADTK. In comparing the two rat liver cell lines, GN4 cells exhibited approximately 5-fold greater angiotensin II- and thapsigargin-dependent CADTK activation than WB cells. Although maximal JNK activation by stress-dependent pathways (e.g. UV and anisomycin) was equivalent in the two cell lines, calcium-dependent JNK activation was 5-fold greater in GN4, correlating with CADTK activation. In contrast to JNK, the thapsigargin-dependent calcium signal did not activate mitogen-activated protein kinase and Ang II-dependent mitogen-activated protein kinase activation was not correlated with CADTK activation. Finally, while some stress-dependent activators of the JNK pathway (NaCl and sorbitol) stimulated CADTK, others (anisomycin, UV, and TNFalpha) did not. In summary, cells expressing CADTK/PYK2 appear to have two alternative JNK activation pathways: one stress-activated and the other calcium-dependent.
Text S2: Calculation of average time-delayed cross-correlation (TLXC) from Using a robotic fish to investigate individual differences in social responsiveness in the guppy.
Creators: name: Bierbach, David
name: Landgraf, Tim
name: Romanczuk, Pawel
name: Lukas, Juliane
name: Nguyen, Hai
name: Wolf, Max
name: Krause, Jens
Publisher: name: The Royal Society
Year: 2018
Subjects: subject: 170299 Cognitive Science not elsewhere classified
subject: FOS: Psychology
subject: 90301 Biomaterials
subject: FOS: Medical engineering
subject: 90602 Control Systems, Robotics and Automation
subject: FOS: Electrical engineering, electronic engineering, information engineering
subject: 30402 Biomolecular Modelling and Design
subject: FOS: Chemical sciences

description: Responding towards the actions of others is one of the most important behavioural traits whenever animals of the same species interact. Mutual influences among interacting individuals may modulate the social responsiveness seen and thus make it often difficult to study the level and individual variation in responsiveness. Here, open-loop biomimetic robots that provide standardized, non-interactive social cues can be a useful tool. These robots are not affected by the live animal's actions but are assumed to still represent valuable and biologically relevant social cues. As this assumption is crucial for the use of biomimetic robots in behavioural studies, we hypothesized (i) that meaningful social interactions can be assumed if live animals maintain individual differences in responsiveness when interacting with both a biomimetic robot and a live partner. Furthermore, to study the level of individual variation in social responsiveness, we hypothesized (ii) that individual differences should be maintained over the course of multiple tests with the robot. We investigated the response of live guppies (Poecilia reticulata) when allowed to interact either with a biomimetic open-loop-controlled fish robot—‘Robofish’—or with a live companion. Furthermore, we investigated the responses of live guppies when tested three times with Robofish. We found that responses of live guppies towards Robofish were weaker compared with those of a live companion, most likely as a result of the non-interactive open-loop behaviour of Robofish. Guppies, however, were consistent in their individual responses between a live companion and Robofish, and similar individual differences in response towards Robofish were maintained over repeated testing even though habituation to the test environment was detectable. Biomimetic robots like Robofish are therefore a useful tool for the study of social responsiveness in guppies and possibly other small fish species.
Acylglycerol Recycling from Triacylglycerol to Phospholipid, Not Lipase Activity, Is Defective in Neutral Lipid Storage Disease Fibroblasts
Creators: name: Coleman, Rosalind A.
name: Ariel Igal, R.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: Neutral lipid storage disease (NLSD) is an autosomal recessive disorder in which excess triacylglycerol (TG) accumulates in most cells. Although it has been hypothesized that the TG accumulation is caused by a functional defect in cytosolic lipase activity, we were able to expose TG hydrolysis in NLSD cells by using triacsin C, an inhibitor of acyl-CoA synthetase that blocks the reincorporation of hydrolyzed fatty acids into glycerolipids. Our data suggest that TG lipolysis in NLSD cells is masked by rapid TG resynthesis, occurring because released acylglycerols cannot be used for phospholipid synthesis. In uptake studies, triacsin C blocked the incorporation of [3H]glycerol into glycerolipids, incorporation of [14C]oleate into TG, but not incorporation of [14C]oleate into phospholipid. Thus, the drug inhibited both de novo synthesis of glycerolipids via the glycerol-3-phosphate pathway and the synthesis of TG from diacylglycerol. The drug did not appear to block reacylation of lysophospholipids. Triacsin C caused a loss of about 60% of the TG mass from both NLSD and oleate-loaded control cells. Rates of TG lipolysis were similar in NLSD cells and oleate-loaded control cells labeled with [6-(7-nitro-2,1,3-benzoxadiazol-4-yl)-amino]hexanoic acid or labeled with [14C]oleate or [3H]glycerol and chased in the presence of triacsin C. During a 96-h chase, [14C]oleate reincorporation into the different phospholipid species increased only in control cells. Similar results were observed when NLSD, and control cells were chased after labeling with [3H]glycerol. These data strongly suggest that normal human fibroblasts mobilize stored TG for phospholipid synthesis and that recycling to PC occurs via a TG-derived mono- or diacylglycerol intermediate. Normal recycling to phosphatidylethanolamine may primarily involve TG-derived acyl groups rather than an acylglycerol precursor. NLSD cells appear to have a block in this recycling pathway with the result that both hydrolyzed fatty acids and the acylglycerol backbone are re-esterified to form TG. Because the NLSD phenotype includes ichthyosis, fatty liver, myopathy, cardiomyopathy, and mental retardation, the recycling pathway appears to be critical for the normal function of skin, liver, muscle, heart, and the central nervous system.
(2027) Paulina Sykes
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
In-plane resonant excitation of quantum dots in a dual-mode photonic-crystal waveguide with high $β$-factor
Creators: name: Zhou, Xiaoyan
name: Lodahl, Peter
name: Midolo, Leonardo
Publisher: name: arXiv
Year: 2021
Subjects: subject: Quantum Physics (quant-ph)
subject: Optics (physics.optics)
subject: FOS: Physical sciences

description: A high-quality quantum dot (QD) single-photon source is a key resource for quantum information processing. Exciting a QD emitter resonantly can greatly suppress decoherence processes and lead to highly indistinguishable single-photon generation. It has, however, remained a challenge to implement strict resonant excitation in a stable and scalable way, without compromising any of the key specs of the source (efficiency, purity, and indistinguishability). In this work, we propose a novel dual-mode photonic-crystal waveguide that realizes direct in-plane resonant excitation of the embedded QDs. The device relies on a two-mode waveguide design, which allows exploiting one mode for excitation of the QD and the other mode for collecting the emitted single photons with high efficiency. By proper engineering of the photonic bandstructure, we propose a design with single-photon collection efficiency of $β> 0.95$ together with a single-photon impurity of $ε< 5 \times 10^{-3}$ over a broad spectral and spatial range. The device has a compact footprint of $\sim 50$ $μ$m$^2$ and would enable stable and scalable excitation of multiple emitters for multi-photon quantum applications.
Additional file 1 of What helps older people persevere with yoga classes? A realist process evaluation of a COVID-19-affected yoga program for fall prevention
Creators: name: Haynes, Abby
name: Gilchrist, Heidi
name: Oliveira, Juliana S.
name: Grunseit, Anne
name: Sherrington, Catherine
name: Lord, Stephen
name: Tiedemann, Anne
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Computational Biology

description: Additional file 1. Research rigour.
LEARNERS' SATISFACTION, LEARNING EXPERIENCE, AND DEVELOPING CRITICAL THINKING SKILLS IN THE POST-PANDEMIC ERA: AN  INVESTIGATION OF BLENDED LEARNING  OF BSED-SCIENCE PROGRAM WITH  ON-CAMPUS CLASSES  AT GORDON  COLLEGE
Creators: name: Andrin, John Michael
name: Ibanez, Adrian
name: Lindayag, Shandriel
name: Geniza, Ginalyn
Publisher: name: The APO
Year: 2024
Subjects: Blended Learning
Learner Satisfaction
Learning Experiences
Critical Thinking Skills
Post-Pandemic Education

description: This study examines the effectiveness of blended learning for science majors at Gordon College in the post-pandemic era. It investigates learner satisfaction, learning experiences, and critical thinking skill development through the blended learning approach. The study involves 50 BSEd-Science major students and data were collected through survey questionnaires. The results reveal that most respondents prefer blended learning and express overall satisfaction, appreciating its benefits, especially regarding accessibility and convenience. Blended learning is perceived to support critical thinking skill development by fostering information synthesis and the ability to ask significant questions. Significant relationships were observed between learning experience and age, as well as between learning experience and year level. However, no significant relationships were found for learner satisfaction or critical thinking skill development based on individual profiles. The study concludes that blended learning is a practical educational approach for science majors, providing valuable insights for educational practices and improving learning outcomes.
Analysis of Protein Structure-Function in Vivo: ADENOVIRUS-MEDIATED TRANSFER OF LIPASE LID MUTANTS IN HEPATIC LIPASE-DEFICIENT MICE
Creators: name: Parrott, Catherine
name: Kashyap, Vikram S.
name: Santamarina-Fojo, Silvia
name: Dugi, Klaus A.
name: Duarte, Cornelio
name: Brown, David R.
name: Kobayashi, Junji
name: Maeda, Nobuyo
name: Applebaum-Bowden, Deborah
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: Hepatic lipase (HL) and lipoprotein lipase (LPL) are key enzymes involved in the hydrolysis of triglycerides and phospholipids present in circulating plasma lipoproteins. Despite their similarities, the role that each of these two lipases play in the metabolism of triglyceride-rich lipoproteins and high density lipoproteins is distinct. In order to identify structural domains that may confer the different substrate specificities between HL and LPL, we have utilized a novel approach for performing structure-function analysis of a protein, in vivo, by using recombinant adenovirus vectors to express native and mutant enzymes in an animal model for a human genetic deficiency. HL-deficient mice (n = 19) characterized by increased plasma cholesterol and phospholipid concentrations were injected with adenovirus expressing luciferase (rLucif-AdV), native hepatic (rHL-AdV), and lipoprotein lipase (rLPL-AdV) or lipase mutants in which the lid covering the catalytic site of either enzyme was exchanged (rHL+LPL lid-AdV and rLPL+HL lid-AdV). Mice injected with rLucif-AdV had no changes in post-heparin HL and LPL activities (217 +/- 29 and 7 +/- 2 nmol/min/ml, respectively) as well as plasma lipids. Despite expression of similar levels of post-heparin plasma lipase activity on day 5 post-adenovirus infusion (9806 +/- 915 and 9677 +/- 2033 nmol/min/ml, respectively) mice injected with rHL-AdV or rHL+LPL lid-AdV demonstrated marked differences in the reduction of plasma phospholipids (70% and 32%, respectively, p < 0.005). Similarly, despite post-heparin plasma lipolytic activities of 4495 +/- 534 and 4844 +/- 1336 nmol/min/ml, injection of rLPL-AdV or rLPL+HL lid-AdV resulted in phospholipid reductions of 31% and 81% (p < 0.005). Exchange of the lipase lid did not significantly alter plasma triglyceride concentrations. Thus, preferential in vivo hydrolysis of phospholipids was demonstrated in animals expressing lipases containing the HL lid but not the LPL lid. These studies identify the lipase lid as a major structural motif responsible for conferring the different in vivo phospholipase activities between HL and LPL, a function which may modulate the distinct physiological roles of these two similar lipolytic enzymes in lipoprotein metabolism. The use of recombinant adenovirus to express mutant proteins in animal models for human genetic deficiencies represents a powerful, new approach for performing structure-function analysis of proteins in vivo.
(2028) Catherine Eliza Sipling
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.