Additional file 2 of Population genetics and molecular xenomonitoring of Biomphalaria freshwater snails along the southern shoreline of Lake Malawi, Malawi
Creators: name: Archer, John
name: Cunningham, Lucas J.
name: Juhász, Alexandra
name: Jones, Sam
name: Reed, Amber L.
name: Yeo, Shi Min
name: Mainga, Bright
name: Chammudzi, Priscilla
name: Kapira, Donales R.
name: Lally, David
name: Namacha, Gladys
name: Makaula, Peter
name: LaCourse, James E.
name: Kayuni, Sekeleghe A.
name: Webster, Bonnie L.
name: Musaya, Janelisa
name: Stothard, J. Russell
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Molecular Biology
subject: Environmental Sciences not elsewhere classified
subject: Ecology
subject: FOS: Biological sciences
subject: Science Policy
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Virology
subject: Computational Biology

description: Additional file 2: Table S1A. Primer sequences used to detect and amplify a 700-bp fragment of the Biomphalaria spp. mitochondrial cytochrome oxidase subunit 1 (cox1) gene. Table S1B. Reaction mix used to carry out endpoint PCR to detect and amplify a 700-bp fragment of the Biomphalaria spp. mitochondrial cytochrome oxidase subunit 1 (cox1) gene. Table S1C. PCR conditions used to carry out endpoint PCR to detect and amplify a 700-bp fragment of the Biomphalaria spp. mitochondrial cytochrome oxidase subunit 1 (cox1) gene. Table S2A. Primer and probe sequences used to amplify a 956-bp region of the Schistosoma spp. mitochondrial cytochrome oxidase subunit 1 (cox1) gene. Table S2B. Reaction mix used to carry out end-point targeting a 956-bp region of the Schistosoma spp. mitochondrial cytochrome oxidase subunit 1 (cox1) gene. Table S2C. PCR conditions used to carry out end-point targeting a 956 bp region of the Schistosoma spp. mitochondrial cytochrome oxidase subunit 1 (cox1) gene. Table S3A. Primer and probe sequences used to amplify the complete Schistosoma spp. nuclear internal transcribed spacer region (inclusive of both ITS regions 1 + 2 and the nuclear 5.8S region). Table S3B. Reaction mix used to carry out end-point PCR targeting the complete Schistosoma spp. nuclear internal transcribed spacer region (inclusive of both ITS regions 1 + 2 and the nuclear 5.8S region). Table S3C. PCR conditions used to carry out end-point PCR targeting the complete Schistosoma spp. nuclear internal transcribed spacer region (inclusive of both ITS regions 1 + 2 and the nuclear 5.8S region). Table S4A. Primer sequences used to detect and amplify the complete Biomphalaria spp. and Schistosoma spp. nuclear internal transcribed spacer regions (~ 1250 bp and ~ 1005bp in length, respectively; inclusive of both ITS regions 1 + 2 and the nuclear 5.8S region). Table S4B. Molecular xenomonitoring PCR reaction mix used to detect and amplify the complete Biomphalaria spp. ITS region, the complete Trematoda ITS region and a partial region of the S. mansoni ND5 gene. Table S4C. Molecular xenomonitoring PCR cycling conditions used to detect and amplify the complete Biomphalaria spp. ITS region, the complete Trematoda. ITS region and a partial region of the S. mansoni ND5 gene.