Additional file 1 of Sequential catalytic lignin valorization and bioethanol production: an integrated biorefinery strategy
Creators: name: Wu, Yilu
name: Su, Changsheng
name: Liao, Zicheng
name: Zhang, Gege
name: Jiang, Yongjie
name: Wang, Yankun
name: Zhang, Changwei
name: Cai, Di
name: Qin, Peiyong
name: Tan, Tianwei
Publisher: name: figshare
Year: 2024
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Genetics
subject: Biotechnology
subject: Environmental Sciences not elsewhere classified
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: Biological Sciences not elsewhere classified
subject: Mathematical Sciences not elsewhere classified
subject: Inorganic Chemistry
subject: FOS: Chemical sciences
subject: Plant Biology

description: Additional file 1. Figure S1. (A) Scatter plot of δT versus lignin monomers yields with different ratios of 2-PrOH:H2O (v/v) in initial liquor. (B) Scatter plot of δT versus delignification of pulps with different ratios of 2-PrOH:H2O (v/v) in initial liquor. (C) Scatter plot of RED versus lignin monomers yields with different ratios of 2-PrOH/H2O in initial liquor. (D) Scatter plot of RED versus delignification of pulps with different ratios of 2-PrOH/H2O in initial liquor. Figure S2. SEM images of RCF pulps using the pulping liquor with different 2-PrOH:H2O ratios. (A)-(F) represented to the raw poplar sawdust, and the pulps after RCF using the liquor that consisted of 1:9, 3:7, 5:5. 7:3 and 9:1 of 2-PrOH:H2O (v/v), respectively. Figure S3. Fermentation performances of the parent S. cerevisiae and engineered S. cerevisiae strains for ethanol production using the YPX medium containing 40 g/L of xylose. Time course of (A) OD600, (B) xylose, and (C) ethanol concentration in batch fermentation process. (D) Comparison of ethanol concentration, yield, and OD600max in YPD medium using different strains. Table S1. Sugar concentrations after enzymatic hydrolysis of RCF pulps by different ratios of 2-PrOH:H2O (v/v). Table S2. Yield of RCF oil (wt%) using different ratios of 2-PrOH:H2O (v/v) as initial liquor, in which process 11.70 g dry poplar sawdust was mixture with the initial liquor for before fractionation. Table S3. Hildebrand solubility parameter (δT) and HSP (δD, δP, δH) for selected solvents (2-PrOH:H2O) and poplar lignin. Table S4. Molecular weight distribution of the lignin oil fractionated by different proportion of 2-PrOH:H2O (v/v) in the initial liquor. Table S5. Lignin monomers yield (wt%, lignin monomers yield=lignin monomers mass in the lignin oil / raw poplar sawdust × 100 %) using different proportion of 2-PrOH:H2O (v/v) in initial liquor. Table S6. Types and contents of hydroxyl groups in the RCF oil. Table S7. Crystallinity (CrI %) of the RCF pulps fractionated by different ratios of 2-PrOH:H2O. Table S8. Ethanol fermentation performances by parent S. cerevisiae and the engineered S. cerevisiae using YPX as substrate (40 g/L xylose). Table S9. Ethanol fermentation performances by S. cerevisiae YL23-1 using enzymatic hydrolysates of the RCF pulps. Table S10. Ethanol fermentation performances by S. cerevisiae YL23-1 using synthetic medium, enzymatic hydrolysates of the 5:5 of 2-PrOH:H2O (v/v) ratio group with and without nutrients (YP) addition. Table S11. Saccharomyces cerevisiae, plasmid, gRNA and primers used in this study.