Additional file 1 of Fluorescence-tagged salivary small extracellular vesicles as a nanotool in early diagnosis of Parkinson’s disease
Creators: name: Rastogi, Simran
name: Rani, Komal
name: Rai, Sanskriti
name: Singh, Rishabh
name: Bharti, Prahalad Singh
name: Sharma, Vaibhav
name: Sahu, Jyoti
name: Kapoor, Vrinda
name: Vishwakarma, Poorvi
name: Garg, Sumit
name: Gholap, Shivajirao Lahu
name: Inampudi, Krishna Kishore
name: Modi, Gyan Prakash
name: Rani, Neerja
name: Tripathi, Madhavi
name: Srivastava, Achal
name: Rajan, Roopa
name: Nikolajeff, Fredrik
name: Kumar, Saroj
Publisher: name: figshare
Year: 2023
Subjects: subject: Biophysics
subject: Biochemistry
subject: Space Science
subject: Physical Sciences not elsewhere classified
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Molecular Biology
subject: Neuroscience
subject: Biotechnology
subject: Immunology
subject: FOS: Clinical medicine
subject: Virology

description: Additional file 1: Fig. S1. [Western blot of anti-CD63. In this blot, the pellet obtained during the sEV isolation method and the supernatant, show the purity of sEVs. P= Pellet obtained by the PEG-based precipitation combined with ultrafiltration. S= Supernatant obtained during the isolation process]. Fig. S2. [Expression profile of CD9 in PD patients (P1-P4) and healthy controls (C1-C3). (A) Western blot of anti-CD9 and anti-GAPDH with equal sample volume (5µl) in PD and HC. Densitometric analysis of (B) anti-CD9 (p=0.0004) and (C) anti-GAPDH (p=0.0137). GAPDH is a loading control. All graphs are presented with Mean ± SEM]. Fig. S3. [Expression profile of CD63 in PD patients (P1-P4) and healthy controls (C1-C3). (A) Western blot of anti-CD63 and anti-GAPDH with equal sample volume (5µl) in PD and HC. Densitometric analysis of (B) anti-CD63 (p=0.0017) and (C) anti-GAPDH (p=0.047). GAPDH is a loading control. All graphs are presented with Mean ± SEM]. Fig. S4. [Expression profile of Flotillin-1 in PD patients (P1-P4) and healthy controls (C1-C3). (A) Western blot of anti- Flotillin-1 and anti-GAPDH with equal sample volume (5µl) in PD and HC. Densitometric analysis of (B) anti-Flotillin-1 (p=0.0213) and (C) anti-GAPDH (p=0.0448). GAPDH is a loading control. All graphs are presented with Mean ± SEM]. Fig. S5. [Expression profile of L1CAM in PD patients (P1-P4) and healthy controls (C1-C3). (A) Western blot of anti-L1CAM and anti-GAPDH with equal sample volume (5µl) in PD and HC. Densitometric analysis of (B) anti-L1CAM (p=0.0253) and (C) anti-GAPDH (p=0.0399). GAPDH is a loading control. All graphs are presented with Mean ± SEM]. Fig. S6. [Expression profile of Phospho-α-Synuclein in PD patients (P1-P4) and healthy controls (C1-C3). (A) Western blot of anti-phospho-α-Synuclein and anti-GAPDH with equal sample volume (5µl) in PD and HC. Densitometric analysis of (B) anti- phospho-α-Synuclein (p=0.0093) and (C) anti-GAPDH. GAPDH is a loading control. All graphs are presented with Mean ± SEM]. Fig. S7. [Expression profile of CD9 with Concentration Normalization in PD patients (P1-P4) and healthy controls (C1-C3). (A) Western blot of anti-CD9 and anti-GAPDH with equal protein sample loading (2.5µg) in PD and HC. Densitometric analysis of (B) anti-CD9 and (C) anti-GAPDH. GAPDH is a loading control. We did not observe significant differences in CD9 and GAPDH expressions in PD and HC. All graphs are presented with Mean ± SEM]. Fig. S8. [Correlation coefficient analysis between sEV concentration and UPDRS score in controls and PD patients].