MOESM1 of Characterization of type I and type II diacylglycerol acyltransferases from the emerging model alga Chlorella zofingiensis reveals their functional complementarity and engineering potential
Creators: name: Mao, Xuemei
name: Wu, Tao
name: Kou, Yaping
name: Shi, Ying
name: Zhang, Yu
name: Liu, Jin
Publisher: name: figshare
Year: 2019
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Genetics
subject: Molecular Biology
subject: Evolutionary Biology
subject: Ecology
subject: Biological Sciences not elsewhere classified
subject: Information Systems not elsewhere classified
subject: Developmental Biology
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Plant Biology

description: Additional file 1: Figure S1. Validation of incorrect assembly of part of Chr09. a, b Part of Chr03 (a) and Chr09 (b) viewed in JBrowse. c Sequence blast and PCR validation. Chr03 and Chr09 share a 35-kb sequence, which encodes ten genes including CzDGTT3 (Cz03g14070+Cz03g14080 or Cz09g23010+Cz09g23020). We inferred a wrong assembly of this part may occur for Chr03 or Chr09. Primer pairs were designed for Chr03 (f1+r1) and Chr09 (f2+r1), respectively; only the former gave the expected PCR product, while the latter had no product (c), indicating the incorrect assembly of Chr09. Figure S2. Comparison between the gene models of DGATs predicted from Roth et al. [31] and ours confirmed by 5’-RACE and sequencing. The gene models from Roth et al. [31] and us are on the top and bottom of each panel, respectively. The red arrows indicate the primers used for cloning the full-length coding sequence. Note that based on the sequence of Cz11921100, PCR using cDNA as the template gave no product. Figure S3. Phylogenetic analysis showing relationships among CzDGATs and various annotated DGATs from plants, animal, fungi and microalgae. Alignment of amino acid sequences was conducted using ClustalX 2.1. The tree was generated in the MEGA6.0 software using the maximum-likelihood method. Protein sequences were used for calculations. The percentages of bootstrap support that was calculated from 1000 bootstrap resamplings are shown on the branches. Bootstrap values &gt;40% are shown above the branch. The sources and Genbank Accession numbers of DGATs are shown in the brackets. Figure S4. Predicated transmembrane domains for NoDGAT1A NoDGAT1B by TMHMM (V2.0, http://www.cbs.dtu.dk/services/TMHMM/). Figure S5. Yeast one-hybrid assay identified TFs bound to the promoter region of CzDGAT1A and CzDGTT5. (-) and (+) designate the negative and positive control, respectively. Blue indicates the binding between the promoter and TF. Figure S6. Genomic map of CzDGAT1A viewed in JBrowse (a) and predicted binding sites of MYB (b) and bZIP (c) at the 2.0-kb CzDGAT1A promoter. The underlined sequences in (b) and (c) indicate the 5’ of neighboring gene, Cz06g05020. The TFmatrixIDs were shown in Additional file 3: Data S1. Figure S7. Genomic map of CzDGTT5 viewed in JBrowse (a) and predicted binding sites of MYB (b) and bZIP (c) at the 2.0-kb CzDGTT5 promoter. The underlined sequences in (b) and (c) indicate the 3’ of neighboring gene, Cz09g27300. The TFmatrixIDs were shown in Additional file 3: Data S1. Figure S8. Gene expression of MYB1, bZIP3, CzDGAT1A, CzDGTT5, Cz06g05020 and Cz09g27300 at 0 and 12 h of ND. Cz06g05020 and Cz09g27300 are the neighboring genes of CzDGAT1A and CzDGTT5, respectively (Additional file 1: Figure S6 and Figure S7).