Additional file 1 of Organic amendments alleviate early defoliation and increase fruit yield by altering assembly patterns and of microbial communities and enzymatic activities in sandy pear (Pyrus pyrifolia)
Creators: name: Kang, Yalong
name: An, Xiangrui
name: Ma, Yanwei
name: Zeng, Shaomin
name: Jiang, Shangtao
name: Wu, Wenli
name: Xie, Changyan
name: Wang, Zhonghua
name: Dong, Caixia
name: Xu, Yangchun
name: Shen, Qirong
Publisher: name: figshare
Year: 2021
Subjects: subject: Microbiology
subject: FOS: Biological sciences

description: Additional file 1: Table S1. Fertilization protocol from 2017 to 2019. CK, conventional fertilization; CK-HA, conventional fertilization combined with humic acid (HA); BIO, bio-organic fertilizer; BIO-HA, mixed application of BIO and HA. Table S2. Extracellular enzymes with corresponding commission number (EC), corresponding substrate, and the abbreviation used in this study. Table S3. Effect of organic amendments on the leaf nutrient concentrations of sandy pear. Each value represents the mean (n = 6), and standard error values are indicated with ± . Different letters indicate significant difference (P &lt; 0.05) in every row among the four treatments as determined by Fisher’s least significant difference test (LSD) at α = 0.05. Table S4. Network topological characteristics calculated by Network Analyzer tool in Gephi 0.9.2. Figure S1. Spearman correlations show the relationships among different functional groups. Shades of blue and red represent a negative and positive correlation coefficient (r), respectively. Empty grids mean no difference. The detailed traits of the different functional groups can be found in the data analysis section described in the article. Figure S2. Effects of organic amendments on (A) SPAD, (B) leaf area and (C) leaf thickness. Each value represents the mean (n = 6), and the error bars are the standard errors. Significant differences are indicated by different lowercase letters at P &lt; 0.05 based on the LSD test. Figure S3. Nutrient-cycle enzyme activities across experimental treatments. αG, α-1,4-Glucosidase; βG, β-1,4-Glucosidase; βX, β-1,4-Xylosidase; CBH, β-D-Cellobiohydrolase; LAP, Leucine amino peptidase; NAG, β-1,4-N-Acetyl-glucosaminidase; ACP, Acid phosphomonoesterase; PeO, Peroxidas; PhO, Phenol oxidase. Different lowercase letters indicate significant difference at 0.05 levels (LSD, P &lt; 0.05) among different fertilizer treatments. The error bars are the standard errors (n = 6). Figure S4. Bacterial (A and B) and fungal (C and D) diversity by fertilization treatment 2018 to 2019. Different lowercase letters indicate significant difference at 0.05 levels (LSD, P &lt; 0.05) among different treatments. The error bars are the standard errors (n = 6). Figure S5. Significance analysis of the relative abundances of the 15-dominant bacterial (A) and fungal (B) phyla based on the LSD at 0.05 levels in pear orchard soils in 2018—2019. * indicating that there are significant differences between CK and CK-HA, BIO and BIO-HA, respectively. Figure S6. Network analysis revealing the co-occurrence pattern between bacterial and fungal OTUs in -HA (CK and BIO) and + HA (CK-HA and BIO-HA) treated soils, 2018 and 2019. Colored nodes signify corresponding OTUs assigned to major phyla. Red line and blue line represent strong positive linear (r &gt; 0.6) and strong negative linear (r &lt; -0.6) and relationships, respectively. The size of each node is proportional to the number of connections (degree).