Additional file 1 of Identification and engineering of 32 membered antifungal macrolactone notonesomycins
Creators: name: Falicia Goh
name: Mingzi M. Zhang
name: Lim, Tian Ru
name: Low, Kia Ngee
name: Choy Eng Nge
name: Heng, Elena
name: Yeo, Wan Lin
name: Sirota, Fernanda L.
name: Crasta, Sharon
name: Zann Tan
name: Ng, Veronica
name: Leong, Chung Yan
name: Huibin Zhang
name: Lezhava, Alexander
name: Swaine L. Chen
name: Hoon, Shawn S.
name: Eisenhaber, Frank
name: Eisenhaber, Birgit
name: Kanagasundaram, Yoganathan
name: Fong T. Wong
name: Siew Bee Ng
Publisher: name: figshare
Year: 2020
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Genetics
subject: Molecular Biology
subject: Pharmacology
subject: Biotechnology
subject: Evolutionary Biology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: FOS: Chemical sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: Hematology
subject: Plant Biology

description: Additional file 1: Table S1. NMR spectral dataa of notonesomycin A (1) and notonesomycin B (2). Table S2. Annotation of 59 ORFs of the notonesomycin BGC by sequence homology searches using BLASTp against the NCBI nr database. Table S3. Media compositions used in shake flask fermentation. Table S4. Oligos used in this study. Table S5. List of primers used for RT-qPCR. Figure S1. Structure and gross structures of notonesomycins. Selected HMBC correlations of 1 and 2. Figure S2.13C NMR spectrum (methanol-d4, 100 MHz) of Notonesomycin A (1). Figure S3.1H NMR spectrum (methanol-d4, 400 MHz) of Notonesomycin A (1). Figure S4. COSY spectrum (methanol-d4, 400 MHz) of Notonesomycin A (1). Figure S5. HSQC spectrum (methanol-d4, 400 MHz) of Notonesomycin A (1). Figure S6. HMBC spectrum (methanol-d4, 400 MHz) of Notonesomycin A (1). Figure S7.13C NMR spectrum (methanol-d4, 100 MHz) of Notonesomycin B (2). Figure S8.1H NMR spectrum (methanol-d4, 400 MHz) of Notonesomycin B (2). Figure S9. COSY spectrum (methanol-d4, 400 MHz) of Notonesomycin B (2). Figure S10. HSQC spectrum (methanol-d4, 400 MHz) of Notonesomycin B (2). Figure S11. HMBC spectrum (methanol-d4, 400 MHz) of Notonesomycin B (2). Figure S12. Phylogenetic analysis of 59 acyltransferases (AT) amino acid sequences from notonesomycin (Ntc AT), brasilinolides (Nbr AT), PM100117 and PM100118 (GonP AT) BGCs. Figure S13. Proposed biosynthesis pathways for A) 4-amino 3-hydroxybenzoic acid and B) deoxysugars. Figure S14. Sequence analysis of glycosyltransferases Nbc18 and Nbc22 from the notonesomycin BGC with known glycosyltransferases. Figure S15. Sequence analysis of cytochrome P450 enzymes (Nbc33, Nbc36 and Nbc37) from the notonesomycin BGC with other cytochrome P450 with either hydroxylation or epoxidation function. Figure S16. Sequence analysis of methyltransferases Nbc1 and Nbc56 from Streptomyces sp. A793 present in the notonesomycin BGC with other known O-, N- and C-methyltransferases. Figure S17. LC–MS spectra (base peak chromatogram, BPC) for wild type and nbc20 and nbc21 double KO mutant. Figure S18. Sequence analysis of sulfotransferase Nbc48 from the notonesomycin BGC with sulfotransferases that have been associated with other BGCs.