Additional file 1 of Donor age and body weight determine the effects of fecal microbiota transplantation on growth performance, and fecal microbiota development in recipient pigs
Creators: name: Wang, Xiaofan
name: Tsai, Tsungcheng
name: Zuo, Bin
name: Wei, Xiaoyuan
name: Deng, Feilong
name: Li, Ying
name: Maxwell, Charles V.
name: Yang, Hua
name: Xiao, Yingping
name: Zhao, Jiangchao
Publisher: name: figshare
Year: 2022
Subjects: subject: Space Science
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: Developmental Biology

description: Additional file 1: Table S1. Nursery diet composition (as fed). Table S2. Growing and finishing diet composition (as fed). Table S3. List of age-associated bacterial features identified by LEfSe. Fig. S1. Longitudinal dynamics of alpha diversities (chao1, observed_features, and Shannon) of the gut microbiota in recipient pigs gavage fed with PBS with 20% glycerol (Con), fecal microbiota from a nursery pig with low (NL) or high body weight (NH), fecal microbiota from a growing pig with low (GL) or high body weight (GH), and fecal microbiota from a finishing pig with low (FL) or high body weight (FH) at weaning (a). Alpha diversities (chao1, observed_features, and shannon) of the six donors (b). Fig. S2. Bray-Curtis dissimilarity-based PCOA plots of gut microbial communities from donors and recipient pigs on d25, 35, 49, 63, and 174. Points represent the gut microbiota in recipient pigs gavage fed with PBS with 20% glycerol (Con/yellow), fecal microbiota from a nursery pig with low (NL/light red) or high body weight (NH/dark red), fecal microbiota from a growing pig with low (GL/light green) or high body weight (GH/dark green), and fecal microbiota from a finishing pig with low (FL/light purple) or high body weight (FH/dark purple) at weaning (a). Donors are yellow color filled triangles (light BW) and squares (heavy BW). Fig. S3. The top 50 estimated age (EMA)-associated features identified by the regression-based random forest. Fig. S4. Boxplots present the estimated microbiota ages (EMA) of the gut microbiota in recipient pigs on d 32, 35, 49, 63, and 104 (a). Distributions of EMA values from each group on d35 and 49 (b). The relative abundances of potential modulated bacterial members associated with EMA on d35 and 49 by fecal microbial transplantations (c). Fig. S5. Boxplots showing the relative abundances of BW-associated bacterial features at each treatment (a. d63 and 104; b. d35; c. d49). The scatter plots with regression line showing correlations of these features with body weight (BW) on each day. Box color represents different FMT groups (Con/blue: vehicle group; NL/light red: nursery stage microbiota from a light BW donor; NH/dark red: nursery stage microbiota from a heavy BW donor; GL/light green: growing stage microbiota from a light BW donor; GH/dark green: growing stage microbiota from a heavy BW donor; FL/light red: finishing stage microbiota from a light BW donor; FH/dark red: finishing stage microbiota from a heavy BW donor). Fig. S6. Abundances of the predicted metabolic pathways in the recipient fecal microbiotas that were gavage fed either a placebo or growing stage microbiotas from a heavy body weight donor (GH) on d104. Fig. S7. Abundances of the influenced metabolic pathways predicted in the recipient microbiotas that were gavage fed growing stage microbiotas from both light (GL) and heavy body weight donors (GH) on d49. Fig. S8. Abundances of the influenced metabolic pathways predicted in the recipient microbiotas that were gavage fed either placebo or growing stage microbiotas from a heavy body weight donor (GH) on d49.