MICROBIOLOGICAL, IMMUNOLOGICAL, AND PHYSIOLOGICAL EFFECTS OF LONGEVITY SPINACH (GYNURA PROCUMBENS): IN-VITRO AND IN-VIVO
Creators: name: Mumu, Sinthia Kabir
Publisher: name: Purdue University Graduate School
Year: 2022
Subjects: Animal cell and molecular biology
Animal immunology
Animal physiology - cell
Animal physiology - systems
Oncology and carcinogenesis not elsewhere classified
Biochemistry and cell biology not elsewhere classified
Immunology not elsewhere classified
Microbiology not elsewhere classified

description: Longevity spinach, <em>Gynura procumbens</em>, has been traditionally used to treat a variety of diseases such as eruptive fevers, rash, kidney disease, migraines, constipation, hypertension, diabetes, and cancer. Recently, several papers have reported that this plant also has anti–Herpes simplex virus, anti–hyperglycemic, anti–hyperlipidemic, anti–inflammatory, analgesic, and reduced blood hypertension properties. However, to the best of our knowledge, nothing has been done to modulate stress in aquatic animals using <em>G. procumbens</em>. Therefore, we have investigated the potential of <em>Gynura </em>to modulate stress and immune responses in fish, tilapia. I examined the effects of <em>Gynura </em>by well and disc-diffusion methods against nine selected bacteria. In the well diffusion method, <em>Gynura </em>in ethanol extract showed significant sensitivity against <em>E. feacalis </em>(<em>p</em>&lt;0.05) and <em>P. aeruginosa </em>(<em>p</em>&lt;0.05) with the presence of zone of inhibition. In the disc diffusion method, <em>Gynura </em>in ethanol extract showed significant sensitivity against <em>B. subtilis </em>(<em>p</em>&lt;0.05), <em>S. aureus </em>(<em>p</em>&lt;0.05), and <em>S. epidermidis </em>(<em>p</em>&lt;0.05). Similarly, <em>Gynura </em>in methanol extract also showed significant sensitivity against <em>B. subtilis </em>(<em>p</em>&lt;0.05) and <em>S. aureus </em>(<em>p</em>&lt;0.05). I used different concentrations of ethanol and aqueous extracts of <em>Gynura </em>to investigate its effects on the inhibition of B16 murine melanoma cells and proliferation of spleen cells collected from BALB/c mice. In these experiments, 4g/mL and 0.8g/mL of ethanolic extract significantly inhibited the melanoma cell growth in-vitro (<em>p</em>&lt;0.05). However, there was neither significant proliferation nor inhibition of spleen cells ethanol or aqueous extracts of <em>Gynura </em>with different concentration (<em>p</em>&lt;0.05). I used <em>Gynura-</em>supplemented feed to prevent stress and to treat stressed fish for both short and long propagation periods. In these experiments, <em>Gynura </em>significantly prevented the stress hormone, cortisol, from increasing in the prevention group (<em>p</em>&lt;0.05) and significantly reduced cortisol in the treatment group (<em>p</em>&lt;0.05). The blood glucose level was decreased in prevention and treatment group compared to control. Because of their correlated effects, improvement of overall immunity was observed in the immunological responses, especially in serum lysozyme activity (<em>p</em>&lt;0.05). <em>Gynura procumbens </em>has the potential to be used as the nutraceuticals to modulate stress and immune responses in vertebrates.