Additional file 1 of Collective analysis of the expression and prognosis for LEM-domain proteins in prostate cancer
Creators: name: He, Tianzhen
name: Zhang, Yulian
name: Li, Xueyu
name: Liu, Caihong
name: Zhu, Guanqun
name: Yin, Xinbao
name: Zhang, Zongliang
name: Zhao, Kai
name: Wang, Zhenlin
name: Zhao, Peng
name: Wang, Ke
Publisher: name: figshare
Year: 2022
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Cancer

description: Additional file 1: Figure S1. The expression of LEM-domain proteins in PRAD. (A-C) The expression levels of ANKLE1, EMD and LEMD2 in different cancer tissues compared 42 to normal tissues in the TIMER database. (D-G) LEMD1, ANKLE2, TMPO and LEMD3 expression level in PRAD tissues compared to normal tissues (RNA-seq data from ATCG PRAD). The number of normal group is 52, the number of tumor group is 499. Compared with indicated group, * p&lt;0.05, ** p&lt;0.01, *** p&lt;0.001, n.s.: no significant difference. Figure S2. The co-expression genes with ANKLE1 in prostate adenocarcinoma (PRAD) from the LinkedOmics database. (A-B) Top 50 genes positively and negatively related to ANKLE1 in PRAD showed, respectively, by heat maps. Red represents positively linked genes and blue represents negatively linked genes. (C) Survival map of the top genes positively and negatively associated with ANKLE1 54 in PRAD. (D-F) GO annotations of ANKLE1 in PRAD cohort. Figure S3. The co-expression genes with EMD in prostate adenocarcinoma (PRAD) from the LinkedOmics database. (A-B) Top 50 genes positively and negatively related to EMD in PRAD showed, respectively, by heat maps. Red represents positively linked genes and blue represents negatively linked genes. (C) Survival map of the top 20 genes positively and negatively associated with EMD in PRAD. 65 (D-F) GO annotations of EMD in PRAD cohort. Figure S4. The co-expression genes with LEMD2 in prostate adenocarcinoma (PRAD) from the LinkedOmics database. (A-B) Top 50 genes positively and negatively related to LEMD2 in PRAD showed, respectively, by heat maps. Red represents positively linked genes and blue represents negatively linked genes. (C) Survival map of the top 20 genes positively and negatively associated with LEMD2 in PRAD. 76 (D-F) GO annotations of LEMD2 in PRAD cohort. Figure S5. Comparison of Kaplan-Meier survival curves of the high and low expression of ANKLE1 in PRAD based on immune cells subgroups. (A–I) High ANKLE1 level enriched in Naïve B cells, resting dendritic cells, M2 macrophages, activated mast cells, neutrophils, M1 macrophage, monocytes, CD8 T cells and resting mast cells exhibited no significant differences in OS in PRAD. Figure S6. Comparison of Kaplan-Meier survival curves of the high and low expression of EMD in PRAD based on immune cells subgroups. (A–I) High EMD level enriched in Naïve B cells, resting dendritic cells, M2 macrophages, activated mast cells, neutrophils, M1 macrophage, monocytes, CD8 T cells and resting mast cells exhibited no significant differences in OS in PRAD. Figure S7. Comparison of Kaplan-Meier survival curves of the high and low expression of LEMD2 in PRAD based on immune cells subgroups. (A–I) High LEMD2 level enriched in Naïve B cells, resting dendritic cells, M2 macrophages, activated mast cells, neutrophils, M1 macrophage, monocytes, CD8 T cells and resting mast cells exhibited no significant differences in OS in PRAD. Figure S8. Raw data for western blot shown in Fig. 3D–F. Table S1. Correlation of ANKLE1, EMD and LEMD2 mRNA expression and prognosis in PRAD with different clinicopathological factors by R project. Table S2. The cox proportional hazard model of ANKLE1/135 EMD/LEMD2 and resting NK cells in PRAD (TIMER). Table S3. Correlation analysis between ANKLE1/155 EMD/LEMD2 and related gene markers of immune cells in prostate cancer samples (n=498) in TIMER 2.0.