Additional file 1 of Increased circulating uric acid aggravates heart failure via impaired fatty acid metabolism
Creators: name: Lou, Bowen
name: Wu, Haoyu
name: Ott, Hannes
name: Bennewitz, Katrin
name: Wang, Chen
name: Poschet, Gernot
name: Liu, Hui
name: Yuan, Zuyi
name: Kroll, Jens
name: She, Jianqing
Publisher: name: figshare
Year: 2023
Subjects: subject: Biophysics
subject: Biochemistry
subject: Physical Sciences not elsewhere classified
subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Physiology
subject: Pharmacology
subject: Chemical Sciences not elsewhere classified
subject: Information Systems not elsewhere classified
subject: Developmental Biology
subject: Cancer
subject: Mental Health
subject: Hematology
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Computational Biology

description: Additional file 1. Fig. S1: Correlations between FFA levels and clinical parameters in HF and control patients. Network analysis between fatty acid and clinical parameters. The color in each cell represents value of correlation calculated by Spearman‘s rank correlation coefficient. *p&lt;0.05, **p&lt;0.01, ***p&lt;0.001. Fig. S2: UA aggravates ISO induced heart failure leads to impaired energy metabolism in zebrafish. A Heatmap of several metabolites in UA, ISO and co-treatment induced heart failure zebrafish larvae at 96 hpf. The color in each cell represents the expression of each zebrafish cluster sample as the scale bar showed, each zebrafish cluster includes 50 zebrafish larvae. B PCA analysis of every sample among each group. C VIP scores of the top metabolites among each group. Fig. S3: Expression analyses of elov2, scd and fads2 in zebrafish after UA and ISO treatment. 1 mM UA treatment and 0.5 mM ISO induced elov2, scd and fads2 mRNA expression in Tg(fli1:EGFP) zebrafish larvae at 72, 96 and 120 hpf. Expression of mRNA was analysed by RT-qPCR and was normalized to both b-actin and b2m, each single dot represents one zebrafish cluster’s data, which includes 20–30 zebrafish larvae. For statistical analysis one-way ANOVA followed by Sidak’s multiple comparison test was applied, *p &lt; 0.05, **p &lt; 0.01, ***p&lt;0.001, ****p &lt; 0.0001. Fads2, fatty acid desaturase 2; elov2. fatty acid elongase 2; scd, stearoyl-CoA desaturase. Fig. S4: Heatmap of fatty acids in HepG2 after UA treatment and Srebp1 knockdown. Heatmap of fatty acids in HepG2 after UA treatment and Srebp1 knockdown. The color in each cell represents expression of each cell culture sample as the scale bar showed, n = 3–4 per group. Table S1: Primers. Table S2: Multiple linear regression analysis in the metabolic syndrome cohort (big cohort). Dependent variable: EF value. UA, uric acid; ALT, alanine transaminase; AST, aspartate transaminase; Cre, creatinine; LDL-C, high density lipoprotein; *p &lt; 0.05. Table S3: Baseline information of the heart failure cohort (small cohort for metabolomics detection). EF, ejection fraction; LVEDD, left ventricular end diastolic diameter; LVESD, left ventricular end systolic diameter; UA, uric acid; ALT, alanine transaminase; AST, aspartate transaminase; Cre, creatinine; Chol, cholesterol; TG, triglycerides; HDL-C, high density lipoprotein; LDL-C, high density lipoprotein.