<b>A NOVEL BIOCHEMICAL METHOD FOR PRODUCTION OF AN ANTIBACTERIAL DRUG TRIMETHOPRIM FROM INDUSTRIAL WASTE</b>
Creators: name: Kumar Bompalli, Lenin
Publisher: name: figshare
Year: 2024
Subjects: classificationCode: 310601
subject: Biocatalysis and enzyme technology
classificationCode: 310602
subject: Bioprocessing, bioproduction and bioproducts
classificationCode: 310603
subject: Fermentation
classificationCode: 310604
subject: Industrial biotechnology diagnostics (incl. biosensors)
classificationCode: 310605
subject: Industrial microbiology (incl. biofeedstocks)
classificationCode: 310606
subject: Industrial molecular engineering of nucleic acids and proteins
classificationCode: 310699
subject: Industrial biotechnology not elsewhere classified

description: The novel biochemical method for production of the antibacterial drug trimethoprim from industrial waste involves a sustainable approach to utilize waste materials as starting substrates for synthesizing valuable pharmaceutical compounds. Here’s an overview of the process:<b>1. Identification of Industrial Waste:</b>The process begins with identifying suitable industrial waste materials that contain precursors or compounds that can be chemically transformed into trimethoprim. Examples of such waste materials could include byproducts from pharmaceutical manufacturing, chemical industries, or agricultural residues.<b>2. Biochemical Conversion Process:</b><b>Enzymatic Pathway:</b> Utilizing enzymes or microbial biocatalysts, the waste material undergoes enzymatic conversion to produce intermediates that are crucial for the synthesis of trimethoprim.<b>Microbial Fermentation:</b> Alternatively, microbial fermentation processes may be employed where microorganisms are engineered or selected for their ability to metabolize the waste substrates into desired intermediates.<b>3. Synthesis of Trimethoprim:</b>The intermediates produced from the biochemical conversion are further processed using chemical synthesis or enzymatic steps to yield trimethoprim.Trimethoprim is a synthetic antibacterial agent known for its efficacy against various bacterial infections, particularly in combination with sulfamethoxazole (a sulfonamide antibiotic).<b>4. Purification and Formulation:</b>Post-synthesis, trimethoprim undergoes purification processes to remove impurities and ensure pharmaceutical-grade purity.Formulation steps involve preparing the drug in suitable dosage forms (e.g., tablets, capsules) for medical use.<b>5. Advantages of the Biochemical Method:</b><b>Sustainability:</b> Utilizing industrial waste reduces environmental impact by repurposing materials that would otherwise be discarded.<b>Cost-effectiveness:</b> Accessing raw materials from waste sources can potentially lower production costs compared to traditional chemical synthesis methods.<b>Green Chemistry:</b> The biochemical approach typically involves milder reaction conditions and generates fewer harmful byproducts, aligning with principles of green chemistry.<b>6. Applications and Future Directions:</b>The produced trimethoprim can be used in pharmaceutical formulations to treat bacterial infections.Continued research focuses on optimizing enzymatic pathways, improving yield and efficiency, and exploring new sources of waste materials for sustainable drug production.In conclusion, the novel biochemical method for producing trimethoprim from industrial waste exemplifies a sustainable and innovative approach in pharmaceutical manufacturing, utilizing biocatalysis and waste utilization principles to transform waste into valuable therapeutic agents.