Additional file 1 of Protection of melatonin treatment and combination with traditional antibiotics against septic myocardial injury
Creators: name: Di, Wencheng
name: Jin, Zhenxiao
name: Lei, Wangrui
name: Liu, Qiong
name: Yang, Wenwen
name: Zhang, Shaofei
name: Lu, Chenxi
name: Xu, Xiaoling
name: Yang, Yang
name: Zhao, Huadong
Publisher: name: figshare
Year: 2024
Subjects: subject: Biochemistry
subject: Environmental Sciences not elsewhere classified
subject: Chemical Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Inorganic Chemistry
subject: FOS: Chemical sciences

description: Additional file 1: Fig. S1. Establishment of mouse CLP (a) and aggravated CLP models (b). The cecum was tightly ligated at 1/3 site from its end using 4–0 nylon suture, and double punctures of the cecal wall were performed with a 25 G needle. For the aggravated CLP model, the cecum was tightly ligated at 2/3 site from its end. Fig. S2. Additional echocardiographic data of melatonin pretreatment in septic mice. a LVPWd, LVPWs, HR, and LV mass statistical graphs of the long axis. b LVPWd, LVPWs, HR, and LV mass statistical graphs of the short axis. *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001, ****P &lt; 0.0001 versus Sham or versus CLP or MEL + CLP; ns nonsignificant. n = 6 for each group. Statistical analysis of data was performed using ANOVA. Fig. S3. Additional echocardiographic data on the effect of Compound C for melatonin pretreatment in septic mice. a LVPWd, LVPWs, HR, and LV mass statistical graphs of the long axis. b LVPWd, LVPWs, HR, and LV mass statistical graphs of the short axis. *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001, ****P &lt; 0.0001 versus Sham or versus CLP or MEL + CLP; ns non-significant. n = 6 for each group. Statistical analysis of data was performed using ANOVA. Fig. S4. Additional data of RNA sequencing between the CLP group and the MEL + CLP group. a PCA diagram of each group. b Expression profiles in color indicating significant ones (P &lt; 0.05). Red indicates upregulated and green indicates downregulated. Profile number (top left), gene number (bottom left), and trend (line) in each profile are also labeled. c GO analysis of the upregulated gene categories (P &lt; 0.05). d GO analysis of the downregulated gene categories. e KEGG pathway analysis of upregulated pathways (P &lt; 0.05). f KEGG pathway analysis of downregulated pathways (P &lt; 0.05). Fig. S5. Additional data of RNA sequencing between the sham group and the CLP group. a Differentially expressed mRNAs were displayed by volcano plot. The blue and red parts indicate more than twofold decreased and increased expression of the dysregulated mRNAs in cardiac tissues, respectively (P &lt; 0.05). b Cluster analysis of differential genes in the sham group and the CLP group. c GO analysis of the upregulated gene categories between the sham group and the CLP group. (P &lt; 0.05). d GO analysis of the downregulated gene categories between the sham group and the CLP group (P &lt; 0.05). e KEGG pathway analysis of upregulated pathways between the sham group and the CLP group (P &lt; 0.05). f KEGG pathway analysis of downregulated pathways between the sham group and the CLP group (P &lt; 0.05). Fig. S6. Additional echocardiographic data of melatonin posttreatment in septic mice. a LVPWd, LVPWs, HR, and LV mass statistical graphs of the long axis. b LVPWd, LVPWs, HR, and LV mass statistical graphs of the short axis. *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001, ****P &lt; 0.0001 versus Sham or versus CLP ; ns nonsignificant. n = 6 for each group. Statistical analysis of data was performed using ANOVA. Fig. S7. Protective effect of azithromycin against CLP-induced myocardial injury in mice. a Sepsis scores. b Kaplan–Meier survival curves. Ten animals for each group were used for comparison. Mortality was observed within 72 h. c Blood routine parameters. d Blood biochemical parameters. e Representative echocardiography images of the long axis. f SV, CO, LVEDV, LVESV, LVPWd, LVPWs, HR, and LV mass statistical graphs of the long axis. g Representative echocardiography images of the short axis. h SV, CO, LVEDV, LVESV, LVPWd, LVPWs, HR, and LV mass statistical graphs of the short axis. *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001, ****P &lt; 0.0001 versus Sham or versus CLP; ns nonsignificant. n = 6 for each group. Statistical analysis of data was performed using ANOVA. Fig. S8. Additional echocardiographic data of melatonin combined with azithromycin or vancomycin in septic mice. a LVPWd and LV mass statistical graphs of the long axis. b LVPWd and LV mass statistical graphs of the short axis. c LVPWd and LV mass statistical graphs of the long axis. d LVPWdand LV mass statistical graphs of the short axis. *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001, ****P &lt; 0.0001 versus CLP+AZI or versus CLP + VAN; ns nonsignificant. n = 6 for each group. Statistical analysis of data was performed using t-test. Fig. S9. Protective effect of vancomycin against CLP-induced myocardial injury in mice. a Sepsis scores. b Anal temperature. c Blood routine parameters. d Blood biochemical parameters. e Representative echocardiography images of the long axis. f SV, CO, LVEDV, LVESV, LVPWd, LVPWs, HR, and LV mass statistical graphs of the long axis. g Representative echocardiography images of the short axis. h SV, CO, LVEDV, LVESV, LVPWd, LVPWs, HR, and LV mass statistical graphs of the short axis. *P &lt; 0.05, **P &lt; 0.01, ***P &lt; 0.001, ****P &lt; 0.0001 versus Sham or versus CLP; ns nonsignificant. n = 6 for each group. Statistical analysis of data was performed using AVONA. Additional materials and methods section and table.