cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Mycotoxins in Aquafeeds
Creators: name: Vedant V. Kharabe1, Ashish R. Urkude2, Mitanshu Yadav3
Publisher: name: The Science world a Monthly e magazine
Year: 2024

description: Mycotoxins are a serious problem in food and feed, particularly in fish farming where they can have detrimental effects on the fish species that are farmed. Fishmeal has been successfully substituted with plant-based ingredients in final fish diets. Nevertheless, the use of crops in feeds raises the possibility of mycotoxin and fungal contamination, as well as the frequency of mycotoxicosis in fish. Because mycotoxicosis typically causes decreased body weight, growth impairment, and greater rates of illness and mortality in fish, this could reduce aquaculture's productivity. Furthermore, the muscles of fish may accumulate certain mycotoxins. Because mycotoxins are significant genotoxins, carcinogens, and immunosuppressors to people, eating fish may therefore become another pathway for mycotoxins to infiltrate the human food chain, endangering food security and public health.
Scanning the human proteome for calmodulin-binding proteins
Creators: name: Dong, B.
name: Szostak, J.
name: Liu, R.
name: Valencia, C. A.
name: Shen, X.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2005

description: The calcium ion (Ca2+) is a ubiquitous second messenger that is crucial for the regulation of a wide variety of cellular processes. The diverse transient signals transduced by Ca2+ are mediated by intracellular Ca2+-binding proteins, also known as Ca2+ sensors. A key obstacle to studying many Ca2+-sensing proteins is the difficulty in identifying the numerous downstream target interactions that respond to Ca2+-induced conformational changes. Among a number of Ca2+ sensors in the eukaryotic cell, calmodulin (CaM) is the most widespread and the best studied. Employing the mRNA display technique, we have scanned the human proteome for CaM-binding proteins and have identified and characterized a large number of both known and previously uncharacterized proteins that interact with CaM in a Ca2+-dependent manner. The interactions of several identified proteins with Ca2+/CaM were confirmed by using pull-down assays and coimmunoprecipitation. Many of the CaM-binding proteins identified belong to protein families such as the DEAD/H box proteins, ribosomal proteins, proteasome 26S subunits, and deubiquitinating enzymes, suggesting the possible involvement of Ca2+/CaM in different signaling pathways. The selection method described herein could be used to identify the binding partners of other calcium sensors on the proteome-wide scale.
Integrating Environmental Life Cycle Assessments in the Design Optimization of Electric UAVs
Creators: name:  FELIX POLLET
name:   SCOTT DELBECQ
name:   MARC BUDINGER
name:   JEAN-MARC MOSCHETTA
name:   THOMAS PLANES
Publisher: name: Proceedings of the Aerospace Europe Conference - EUCASS - CEAS - 2023
Year: 2023
Subjects: LIFE CYCLE ASSESSMENT
MULTIDISCIPLINARY DESIGN OPTIMIZATION
UAV

description: As the use and applications of Unmanned Aerial Vehicles (UAVs) continue to grow, it becomes essential to assess their environmental impacts throughout their life cycle. Such assessment would be particularly interesting when the specifications and the technologies of the UAV are not yet frozen. This article aims to address this need by introducing a novel approach that integrates Life Cycle Assessment (LCA) with an existing design framework for sizing electric UAVs. The results show that the production and renewal of batteries is the main contributor to environmental impacts, together with electricity consumption during operation. Several scenarios are presented to explore the sensitivity of the environmental impacts to mission requirements and technological assumptions. The findings provide valuable insights to guide future developments and mitigate environmental impacts from the earliest design stages.
Building Damage-Resilient Dominating Sets in Complex Networks against Random and Targeted Attacks
Creators: name: Molnar, F.
name: Derzsy, N.
name: Szymanski, B. K.
name: Korniss, G.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Physics and Society (physics.soc-ph)
subject: Statistical Mechanics (cond-mat.stat-mech)
subject: Social and Information Networks (cs.SI)
subject: FOS: Physical sciences
subject: FOS: Computer and information sciences

description: We study the vulnerability of dominating sets against random and targeted node removals in complex networks. While small, cost-efficient dominating sets play a significant role in controllability and observability of these networks, a fixed and intact network structure is always implicitly assumed. We find that cost-efficiency of dominating sets optimized for small size alone comes at a price of being vulnerable to damage; domination in the remaining network can be severely disrupted, even if a small fraction of dominator nodes are lost. We develop two new methods for finding flexible dominating sets, allowing either adjustable overall resilience, or dominating set size, while maximizing the dominated fraction of the remaining network after the attack. We analyze the efficiency of each method on synthetic scale-free networks, as well as real complex networks.
Selective butyrylcholinesterase inhibition elevates brain acetylcholine, augments learning and lowers Alzheimer  -amyloid peptide in rodent
Creators: name: Holloway, H. W.
name: Brossi, A.
name: Giordano, T.
name: Lahiri, D. K.
name: Chen, D.
name: Greig, N. H.
name: Yu, Q.-S.
name: Mamczarz, J.
name: Sambamurti, K.
name: Ingram, D. K.
name: Pepeu, G.
name: Furukawa, K.
name: Utsuki, T.
name: Wang, Y.
name: Scali, C.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2005

description: Like acetylcholinesterase, butyrylcholinesterase (BChE) inactivates the neurotransmitter acetylcholine (ACh) and is hence a viable therapeutic target in Alzheimer's disease, which is characterized by a cholinergic deficit. Potent, reversible, and brain-targeted BChE inhibitors (cymserine analogs) were developed based on binding domain structures to help elucidate the role of this enzyme in the central nervous system. In rats, cymserine analogs caused long-term inhibition of brain BChE and elevated extracellular ACh levels, without inhibitory effects on acetylcholinesterase. In rat brain slices, selective BChE inhibition augmented long-term potentiation. These compounds also improved the cognitive performance (maze navigation) of aged rats. In cultured human SK-N-SH neuroblastoma cells, intra- and extracellular β-amyloid precursor protein, and secreted β-amyloid peptide levels were reduced without affecting cell viability. Treatment of transgenic mice that overexpressed human mutant amyloid precursor protein also resulted in lower β-amyloid peptide brain levels than controls. Selective, reversible inhibition of brain BChE may represent a treatment for Alzheimer's disease, improving cognition and modulating neuropathological markers of the disease.
Gefährdet Europa die Enteuropäisierung (und Läuterung) der Kirche?
Creators: name: Voiss, James K.
Publisher: name: Concilium
Year: 1970

description: Concilium, Bd. 40 Nr. 2 (2004): Concilium: Internationale Zeitschrift für Theologie, Band 40, Heft 2
Reciprocity in parity violating non-Hermitian systems
Creators: name: Ghatak, Ananya
name: Mourya, Brijesh Kumar
name: Mandal, Raka Dona Ray
name: Mandal, Bhabani Prasad
Publisher: name: arXiv
Year: 2014
Subjects: subject: Quantum Physics (quant-ph)
subject: High Energy Physics - Theory (hep-th)
subject: FOS: Physical sciences

description: Reciprocity is shown so far only when the scattering potential is either real or parity symmetric complex. We extend this result for parity violating complex potential by considering several explicit examples: (i) we show reciprocity for a PT symmetric (hence parity violating) complex potential which admits penetrating state solutions analytically for all possible values of incidence energy and (ii) reciprocity is shown to hold at certain discrete energies for two other parity violating complex potentials.
description: 11 Pages, 4 figs, Submitted to a Journal for Publication
Modelling and Stability Analysis of Sloshing on Liquid-Propelled Reusable Launch Vehicles
Creators: name:  JOSE ALFREDO MACES-HERNANDEZ
name:   DAVID SEELBINDER
name:   STEPHAN THEIL
Publisher: name: Proceedings of the Aerospace Europe Conference - EUCASS - CEAS - 2023
Year: 2023
Subjects: GNC
FLIGHT CONTROL SYSTEMS
ROBUST CONTROL
H-INFINITY
FLIGHT DYNAMICS
SLOSHING
MULTIBODY DYNAMICS
LAUNCH VEHICLES
REUSABLE LAUNCH VEHICLES

description: The modeling of the liquid dynamics within the tanks of liquid-propelled vehicles is presented in this publication. The proposed model is valid for high-g environments, and planetary atmospheric flight, i.e., applicable to expendable Launch Vehicle (LV)s, Reusable Launch Vehicle (RLV)s and planetary landers. The oscillatory dynamics occurring at the top of the liquid\'s mass is approximated as the sum of n-swinging pendula representing the harmonic response of the n modes. The interaction between the rigid body and pendula is tackled using a classic methodology from multibody systems, which is valid for a generic spacecraft with m tanks and n associated modes, i.e., the iterative Newton-Euler. The rationale behind the computation of the symbolic 3-Degrees of Freedom (DoF) model, and subsequent simplifications to a 2-DoF planar model, is thoroughly discussed. The former model is suitable for simulation, while the latter finds its applications for control design and stability analysis. The symbolic computations were used to validate the Symbolic Multibody Dynamics toolbox belonging to the Guidance, Navigation and Control (GNC) systems department of the German Aerospace Center (DLR). This library can perform analytical computations of Equations of Motion (EoM)s for different configurations of multibody space systems, and at the same time, it is able to provide models that can be easily implemented in simulation environments. The parameters associated to the liquids within the tanks of a space mission are calculated. They were derived using a set of analytical formulas corresponding to the geometry of the propellant and oxidizer tanks of a RLV. The stability analysis of the pair of complex poles and zeros associated to each mode is also studied, furthermore the position of the sloshing masses with regards to the danger zone is computed and analyzed. Finally, a Closed Loop (CL) analysis of the modes for a controller synthesized using 1-{oo designed is presented in terms of the stability margins.
Simultaneous dopamine and single-unit recordings reveal accumbens GABAergic responses: Implications for intracranial self-stimulation
Creators: name: Heien, M. L. A. V.
name: Carelli, R. M.
name: Garris, P. A.
name: Wightman, R. M.
name: Cheer, J. F.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2005

description: Intracranial self-stimulation (ICS) is a motivated behavior that results from contingent activation of the brain reward system. ICS with stimulating electrodes placed in the medial forebrain bundle (MFB) is particularly robust. However, the neurons that course through this pathway use a variety of neurotransmitters including dopamine and GABA. For this reason, the neurotransmitters that are central to this behavior, and the specific roles that they subserve, remain unclear. Here, we used extracellular electrophysiology and cyclic voltammetry at the same electrode in awake rats to simultaneously examine cell firing and dopamine release in the nucleus accumbens (NAc) during ICS and noncontingent stimulation of the MFB. ICS elicited dopamine release in the NAc and produced coincident time-locked changes (predominantly inhibitions) in the activity of a subset of NAc neurons. Similar responses were elicited with noncontingent stimulations. The changes in firing rate induced by noncontingent stimulations were reversed by the GABAA receptor antagonist bicuculline. Most time-locked unit activity was unaffected by D1 or D2-like dopamine-receptor antagonists, or by inhibition of evoked dopamine release, although, for a minority of units, the D1 dopamine-receptor antagonist SCH23390 attenuated neural activity. Thus, neurons in the NAc are preferentially inhibited by GABAA receptors after MFB stimulation, a mechanism that may also be important in ICS.
Spatial regulation of the cAMP-dependent protein kinase during chemotactic cell migration
Creators: name: Baldor, L. C.
name: Howe, A. K.
name: Hogan, B. P.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2005

description: Historically, the cAMP-dependent protein kinase (PKA) has a paradoxical role in cell motility, having been shown to both facilitate and inhibit actin cytoskeletal dynamics and cell migration. In an effort to understand this dichotomy, we show here that PKA is regulated in subcellular space during cell migration. Immunofluorescence microscopy and biochemical enrichment of pseudopodia showed that type II regulatory subunits of PKA and PKA activity are enriched in protrusive cellular structures formed during chemotaxis. This enrichment correlates with increased phosphorylation of key cytoskeletal substrates for PKA, including the vasodilator-stimulated phosphoprotein (VASP) and the protein tyrosine phosphatase containing a PEST motif. Importantly, inhibition of PKA activity or its ability to interact with A kinase anchoring proteins inhibited the activity of the Rac GTPase within pseudopodia. This effect correlated with both decreased guanine nucleotide exchange factor activity and increased GTPase activating protein activity. Finally, inhibition of PKA anchoring, like inhibition of total PKA activity, inhibited pseudopod formation and chemotactic cell migration. These data demonstrate that spatial regulation of PKA via anchoring is an important facet of normal chemotactic cell movement.
The kinetochore protein Ndc10p is required for spindle stability and cytokinesis in yeast
Creators: name: Bouck, D. C.
name: Bloom, K. S.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2005

description: The budding yeast kinetochore is comprised of >60 proteins and associates with 120 bp of centromeric (CEN) DNA. Kinetochore proteins are highly dynamic and exhibit programmed cell cycle changes in localization. The CEN-specific histone, Cse4p, is one of a few stable kinetochore components and remains associated with CEN DNA throughout mitosis. In contrast, several other kinetochore proteins have been observed along interpolar microtubules and at the midzone during anaphase. The inner kinetochore protein, Ndc10p, is enriched at the spindle midzone in late anaphase. We show that Ndc10p is transported to the plus-ends of interpolar microtubules at the midzone during anaphase, a process that requires survivin (Bir1p), a member of the aurora kinase (Ipl1p) complex, and Cdc14p phosphatase. In addition, Ndc10p is required for essential non-kinetochore processes during mitosis. Cells lacking functional Ndc10p show defects in spindle stability during anaphase and failure to split the septin ring during cytokinesis. This latter phenotype leads to a cell separation defect in ndc10–1 cells. We propose that Ndc10p plays a direct role in maintaining spindle stability during anaphase and coordinates the completion of cell division after chromosome segregation.
title: A benzodiazepine activator locks Kv7.1 channels open by electro-mechanical uncoupling
Creators: name: Schreiber, Julian A.
name: Möller, Melina
name: Zaydman, Mark
name: Zhao, Lu
name: Beller, Zachary
name: Becker, Sebastian
name: Ritter, Nadine
name: Hou, Panpan
name: Shi, Jingyi
name: Silva, Jon
name: Wrobel, Eva
name: Strutz-Seebohm, Nathalie
name: Decher, Niels
name: Schmitt, Nicole
name: Meuth, Sven
name: Düfer, Martina
name: Wünsch, Bernhard
name: Cui, Jianmin
name: Seebohm, Guiscard Friedrich Aldous
Publisher: name: University of Muenster, Institutional Repository MIAMI
Year: 2023
Subjects: subject: 610 Medicine and health

description: Loss-of-function mutations in Kv7.1 often lead to long QT syndrome (LQTS), a cardiac repolarization disorder associated with arrhythmia and subsequent sudden cardiac death. The discovery of agonistic IKs modulators may offer a new potential strategy in pharmacological treatment of this disorder. The benzodiazepine derivative (R)-L3 potently activates Kv7.1 channels and shortens action potential duration, thus may represent a starting point for drug development. However, the molecular mechanisms underlying modulation by (R)-L3 are still unknown. By combining alanine scanning mutagenesis, non-canonical amino acid incorporation, voltage-clamp electrophysiology and fluorometry, and in 'silico' protein modelling, we show that (R)-L3 not only stimulates currents by allosteric modulation of the pore domain but also alters the kinetics independently from the pore domain effects. We identify novel (R)-L3-interacting key residues in the lower S4-segment of Kv7.1 and observed an uncoupling of the outer S4 segment with the inner S5, S6 and selectivity filter segments.
description: Finanziert über die DEAL-Vereinbarung mit Wiley 2019-2022.
ReFEx: Reusability Flight Experiment - Aerothermodynamics
Creators: name:  DIVEK SURUJHLAL
name:   VIOLA WARTEMANN
name:   ALEXANDER WAGNER
Publisher: name: Proceedings of the Aerospace Europe Conference - EUCASS - CEAS - 2023
Year: 2023
Subjects: AEROTHERMODYNAMICS
SHOCK-SHOCK INTERACTIONS
SWBLIS
HYPERSONIC

description: The Reusablility Flight Experiment (ReFEx) is currently under development at the German Aerospace Center (DLR). A coupled experimental and numerical campaign was carried out to investigate the surface heating on the ReFEx payload geometry consisting of a forebody and canard during reentry. In this way, numerical tools for a post-flight analysis can be preemptively improved where required. Experiments were undertaken at the High Enthalpy Shock Tunnel Gottingen (HEG) on a 1 :4 scale model with the use of temperature sensitive paint (TSP) on the payload geometry to obtain surface heat flux. The model configuration was varied in angle of attack and canard deflection. A Reynolds-averaged Navier Stokes (RANS) solver in the DLR-TAU code was used for the numerical simulations. This investigation focused on the shock-shock interaction of the nose bow shock with the leading edge shock of the canards. This resulted in significant surface heat flux along the canard. Larger surface heat fluxes were measured in the experiments for the resulting flow downstream of the interaction on the canard, than obtained from the laminar RANS calculations. This was attributed to transition of the boundary layer within the interaction regions and in the presence of significant adverse pressure gradients. Other flow features along the forebody in the vicinity of the canard were qualitatively well-matched by the numerical solutions. This work aims to demonstrate the extent to which the numerical and experimental tools assist useful insights into flow phenomena at reentry conditions for a complex flight geometry, and the aspects for which improvements are required.
A Light Curve Analysis of Classical Novae: Free-free Emission vs. Photospheric Emission
Creators: name: Hachisu, Izumi
name: Kato, Mariko
Publisher: name: arXiv
Year: 2014
Subjects: subject: Solar and Stellar Astrophysics (astro-ph.SR)
subject: FOS: Physical sciences

description: We analyzed light curves of seven relatively slower novae, PW Vul, V705 Cas, GQ Mus, RR Pic, V5558 Sgr, HR Del, and V723 Cas, based on an optically thick wind theory of nova outbursts. For fast novae, free-free emission dominates the spectrum in optical bands rather than photospheric emission and nova optical light curves follow the universal decline law. Faster novae blow stronger winds with larger mass loss rates. Since the brightness of free-free emission depends directly on the wind mass loss rate, faster novae show brighter optical maxima. In slower novae, however, we must take into account photospheric emission because of their lower wind mass loss rates. We calculated three model light curves of free-free emission, photospheric emission, and the sum of them for various WD masses with various chemical compositions of their envelopes, and fitted reasonably with observational data of optical, near-IR (NIR), and UV bands. From light curve fittings of the seven novae, we estimated their absolute magnitudes, distances, and WD masses. In PW Vul and V705 Cas, free-free emission still dominates the spectrum in the optical and NIR bands. In the very slow novae, RR Pic, V5558 Sgr, HR Del, and V723 Cas, photospheric emission dominates the spectrum rather than free-free emission, which makes a deviation from the universal decline law. We have confirmed that the absolute brightnesses of our model light curves are consistent with the distance moduli of four classical novae with known distances (GK Per, V603 Aql, RR Pic, and DQ Her). We also discussed the reason why the very slow novae are about 1 mag brighter than the proposed maximum magnitude vs. rate of decline relation.
description: 31 pages including 36 figures, to appear in ApJ
Galaxy@Sciensano: A toolkit for NGS analysis for applied microbial genomics
Creators: name: Van Braekel, Julien
name: Bogaerts, Bert
name: D'aes, Jolien
name: Godfroid, Maxime
name: Klechtermans, Michaël
name: Delcourt, Thomas
name: De Keersmaecker, Sigrid C.J.
name: Roosens, Nancy H C J
name: Vanneste, Kevin
name: Winand, Raf
Publisher: name: Zenodo
Year: 2023

description: Poster on the Sciensano Galaxy publicly available instance, presented at the ELIXIR Belgium Conference, 2023: "ELIXIR Belgium: Your data, Our services, European success".
Thermodynamics of the pseudogap in cuprates
Creators: name: Tallon, Jeffery
name: Storey, James
Publisher: name: Open Access Te Herenga Waka-Victoria University of Wellington
Year: 2023
Subjects: Uncategorized

description: The key thermodynamic characteristics of the pseudogap state in cuprate superconductors are reviewed. These include YBa2Cu3O7−δ, Y0.8Ca0.2Ba2Cu3O7−δ, YBa2Cu4O8, Bi2Sr2CaCu2O8+δ, La2−xSrxCuO4, and Tl2Ba2CuO4. The electronic specific heat was extracted using a differential technique, and the evolution of the specific-heat coefficient γ and electronic entropy S as a function of temperature, doping, and magnetic field reveals a canonical behavior summarized by the following. The normal-state gap which opens in the pseudogap domain apparently remains open to the highest temperatures investigated. The gap decreases in magnitude with increasing doping and closes abruptly at a critical doping of p ≈ 0.19 holes/Cu, independent of temperature. In this picture, the pseudogap is separated from the pseudogap-free region of the phase diagram by a vertical line similar to the vertical line separating the incoherent and coherent antinodal quasiparticle states found in ARPES. The important role of fluctuations is evident by a diverging enhancement of γ(T) on either side of Tc, and this enables extraction of the mean-field transition temperature (Formula presented.), defining a crescent of parapairing above Tc(p) which extends across the entire superconducting phase diagram and which is quite distinct from pseudogap phenomenology. The data are consistent with d-wave pairing and the BCS ratios are extracted, revealing canonical near-weak-coupling behavior across the over-doped region with a sudden suppression occurring at p ≈ 0.19 when the pseudogap sets in.
The Pseudomonas syringae effector AvrRpt2 cleaves its C-terminally acylated target, RIN4, from Arabidopsis membranes to block RPM1 activation
Creators: name: Singer, A. U.
name: Kim, H.-S.
name: Sondek, J.
name: Desveaux, D.
name: Patel, P.
name: Dangl, J. L.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2005

description: Plant pathogenic Pseudomonas syringae deliver type III effector proteins into the host cell, where they function to manipulate host defense and metabolism to benefit the extracellular bacterial colony. The activity of these virulence factors can be monitored by plant disease resistance proteins deployed to “guard” the targeted host proteins. The Arabidopsis RIN4 protein is targeted by three different type III effectors. Specific manipulation of RIN4 by each of them leads to activation of either the RPM1 or RPS2 disease resistance proteins. The type III effector AvrRpt2 is a cysteine protease that is autoprocessed inside the host cell where it activates RPS2 by causing RIN4 disappearance. RIN4 contains two sites related to the AvrRpt2 cleavage site (RCS1 and RCS2). We demonstrate that AvrRpt2-dependent cleavage of RIN4 at RCS2 is functionally critical in vivo. This event leads to proteasome-mediated elimination of all but a membrane-embedded ≈6.4-kDa C-terminal fragment of RIN4. One or more of three consecutive cysteines in this C-terminal fragment are required for RIN4 localization; these are likely to be palmitoylation and/or prenylation sites. AvrRpt2-dependent cleavage at RCS2, and release of the remainder of RIN4 from the membrane, consequently prevents RPM1 activation by AvrRpm1 or AvrB. RCS2 is contained within the smallest tested fragment of RIN4 that binds AvrB in vitro. Thus, at least two bacterial virulence factors target the same domain of RIN4, a ≈30-aa plant-specific signature sequence found in a small Arabidopsis protein family that may be additional targets for these bacterial virulence factors.
Entiat Cooperative River Basin Study
Creators: name: Economics, Statistics
name: Service, Forest
name: Soil Conservation Service
name: Economics, Statistics
name: Service, Forest
name: Soil Conservation Service
Publisher: name: Unknown
Year: 1979
Subjects: Agricultural and Food Policy
Community/Rural/Urban Development
Crop Production/Industries
Environmental Economics and Policy
Labor and Human Capital
Land Economics/Use
Livestock Production/Industries
Resource /Energy Economics and Policy

description: Excerpts from the report: The Entiat River Basin is located along the eastern slopes of the Cascade Mountains in north-central Washington. The Basin drains directly into the Columbia River, about 20 miles north of the City of Wenatchee in Chelan County. The drainage area is about 266,500 acres. Topography is extremely steep and dissected. Vegetative cover is primarily forest. Eighty-seven percent of the Basin is in public ownership, primarily National Forest. In the lower valley are 1,300 acres of orchardland, much of it classified as prime agricultural land. The overall objective of USDA participation in this Study is to develop an integrated protection plan for the Entiat Basin. It includes development of a program of structural and land treatment measures that primarily address the problems identified by the Entiat Citizens’ Committee. It was determined early in the Study that the concerns of the Citizens’ Committee were attributable to increases (since the 1970 fires) in water runoff, subsequent erosion, and sedimentation. Major study elements, then, were to determine the location, type, and rate of erosion and sedimentation, evaluate their onsite and offsite effects, and develop alternative means of reducing erosion and minimizing adverse effects of sediments. A priority is assigned to proposed remedial and preventative activities which may occur on a variety of fronts. The cost of this activity is related to a predictable, expected reduction in one of the problem areas. The value of this reduction is stated in terms of its effect on stated citizen concerns.
description: The USDA Eastern Washington Cooperative River Basin Study was authorized in 1975. The Forest Service: Economics, Statistics, and Cooperatives Service; and Soil Conservation Service were developing the plan of study when the Entiat need became apparent. At the request of the Entiat Citizens’ Committee, the Entiat River Basin was included as a first priority for study.
Up-regulation of  -catenin by a viral oncogene correlates with inhibition of the seven in absentia homolog 1 in B lymphoma cells
Creators: name: Shackelford, J.
name: Seo, S. Y.
name: Jang, K. L.
name: Pagano, J. S.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2005

description: The protein levels of β-catenin are tightly regulated by the ubiquitin/proteasome system. We provide evidence that two distinct ubiquitin-dependent degradation pathways for β-catenin are active in the same Burkitt's lymphoma cells: Along with the classical glycogen-synthase kinase 3β-dependent destruction machinery, degradation of β-catenin through seven in absentia homolog 1 (Siah-1) ubiquitin ligase is functional in these cells. We show that inhibition of endogenous Siah-1 stabilizes and activates β-catenin in B cells. The principal Epstein–Barr virus oncoprotein, latent membrane protein 1, is involved in β-catenin up-regulation, and expression of latent membrane protein 1 in B lymphoma cells is associated with decreased Siah-1 RNA and protein levels. Thus, we demonstrate the significance of the endogenous Siah-1-dependent ubiquitin/proteasome pathway for β-catenin degradation in malignant human cells and its regulation by a viral oncogene.
TeleFest: Augmented Virtual Teleportation for Live Concerts
Creators: name: Young, Jacob
name: Thompson, S
name: Downer, H
name: Allen, B
name: Pantidi, Konstantia
name: Stoecklein, L
name: Rhee, Taehyun
Publisher: name: Open Access Te Herenga Waka-Victoria University of Wellington
Year: 2023
Subjects: Uncategorized

description: We present TeleFest, a novel system for live-streaming mixed reality 360° videos to online streaming services. TeleFest allows a producer to control multiple cameras in real time, providing viewers with different locations for experiencing the concert, and an intermediate software stack allows virtual content to be overlaid with coherent illumination that matches the real-world footage. TeleFest was evaluated by livestreaming a concert to almost 2,000 online viewers, allowing them to watch the performance from the crowd, the stage, or via a catered experience controlled by a producer in real time that included camera switching and augmented content. The results of an online survey completed by virtual and physical attendees of the festival are presented, showing positive feedback for our setup and suggesting that the addition of virtual and immersive content to live events could lead to a more enjoyable experience for viewers.
Variation in efficiency of DNA mismatch repair at different sites in the yeast genome
Creators: name: Hawk, J. D.
name: Stefanovic, L.
name: Farber, R. A.
name: Boyer, J. C.
name: Petes, T. D.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2005

description: Evolutionary studies have suggested that mutation rates vary significantly at different positions in the eukaryotic genome. The mechanism that is responsible for this context-dependence of mutation rates is not understood. We demonstrate experimentally that frameshift mutation rates in yeast microsatellites depend on the genomic context and that this variation primarily reflects the context-dependence of the efficiency of DNA mismatch repair. We measured the stability of a 16.5-repeat polyGT tract by using a reporter gene (URA3-GT) in which the microsatellite was inserted in-frame into the yeast URA3 gene. We constructed 10 isogenic yeast strains with the reporter gene at different locations in the genome. Rates of frameshift mutations that abolished the correct reading frame of this gene were determined by fluctuation analysis. A 16-fold difference was found among these strains. We made mismatch-repair-deficient (msh2) derivatives of six of the strains. Mutation rates were elevated for all of these strains, but the differences in rates among the strains were substantially reduced. The simplest interpretation of this result is that the efficiency of DNA mismatch repair varies in different regions of the genome, perhaps reflecting some aspect of chromosome structure.
Close to Uniform Prime Number Generation With Fewer Random Bits
Creators: name: Fouque, Pierre-Alain
name: Tibouchi, Mehdi
Publisher: name: arXiv
Year: 2014
Subjects: subject: Cryptography and Security (cs.CR)
subject: Number Theory (math.NT)
subject: FOS: Computer and information sciences
subject: FOS: Mathematics

description: In this paper, we analyze several variants of a simple method for generating prime numbers with fewer random bits. To generate a prime $p$ less than $x$, the basic idea is to fix a constant $q\propto x^{1-\varepsilon}$, pick a uniformly random $a
description: Full version of ICALP 2014 paper. Alternate version of IACR ePrint Report 2011/481
15-Hydroxyprostaglandin dehydrogenase is an in vivo suppressor of colon tumorigenesis
Creators: name: Lawrence, E.
name: Newman, R. A.
name: Dawson, D.
name: Platzer, P.
name: Guda, K.
name: Luo, G.
name: Lovgren, A. K.
name: Myung, S.-J.
name: Dannenberg, A. J.
name: Willis, J.
name: Rerko, R. M.
name: Markowitz, S. D.
name: Yan, M.
name: Pretlow, T. P.
name: Dotson, A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2006

description: 15-Hydroxyprostaglandin dehydrogenase (15-PGDH) is a prostaglandin-degrading enzyme that is highly expressed in normal colon mucosa but is ubiquitously lost in human colon cancers. Herein, we demonstrate that 15-PGDH is active in vivo as a highly potent suppressor of colon neoplasia development and acts in the colon as a required physiologic antagonist of the prostaglandin-synthesizing activity of the cyclooxygenase 2 (COX-2) oncogene. We first show that 15-PGDH gene knockout induces a marked 7.6-fold increase in colon tumors arising in the Min (multiple intestinal neoplasia) mouse model. Furthermore, 15-PGDH gene knockout abrogates the normal resistance of C57BL/6J mice to colon tumor induction by the carcinogen azoxymethane (AOM), conferring susceptibility to AOM-induced adenomas and carcinomas in situ. Susceptibility to AOM-induced tumorigenesis is mediated by a marked induction of dysplasia, proliferation, and cyclin D1 expression throughout microscopic aberrant crypt foci arising in 15-PGDH null colons and is concomitant with a doubling of prostaglandin E2 in 15-PGDH null colonic mucosa. A parallel role for 15-PGDH loss in promoting the earliest steps of colon neoplasia in humans is supported by our finding of a universal loss of 15-PGDH expression in microscopic colon adenomas recovered from patients with familial adenomatous polyposis, including adenomas as small as a single crypt. These models thus delineate the in vivo significance of 15-PGDH-mediated negative regulation of the COX-2 pathway and moreover reveal the particular importance of 15-PGDH in opposing the neoplastic progression of colonic aberrant crypt foci.
Each discipline is different: teacher capabilities for future-focussed digitally infused undergraduate programmes
Creators: name: Sylvester, Allan
name: Starkey, Anne
name: Yates, Anne
name: Randal, John
name: de Róiste, Mairead
name: Lundqvist, Karsten
name: Ormond, Adrianne
Publisher: name: Open Access Te Herenga Waka-Victoria University of Wellington
Year: 2023
Subjects: subject: 130399 Specialist Studies in Education not elsewhere classified
subject: FOS: Educational sciences
subject: 80602 Computer-Human Interaction
subject: FOS: Psychology

description: Disciplines in Higher Education have their own interpretations of what is essential knowledge that infuences what is taught, how teaching occurs, and the role of digital tools. Disciplinary culture is dynamic and evolving, informed by disciplinary research and technology improvement. During the COVID-19 pandemic, digital solutions enabled ongoing teaching when undergraduate courses could not be taught on campus, in lecture theatres, seminar rooms, laboratories, or in the feld. Using digital tools and changes in teaching practices has created a context where Higher Education teachers must consider how future learning and teaching should occur. To explore this, a cross-discipline team used appreciative inquiry framed in complexity theory to examine how teaching in undergraduate programmes is changing in the digital age and implications for Higher Education teachers. The research identifes how digital technologies infuence undergraduate programmes in Applied Statistics, Computer Science, Critical Indigenous Studies, Geography, and Information Systems. Analysis of the case studies identifed how disciplinary culture, context, and technology combine to infuence pedagogical practice and digital capabilities needed to teach in undergraduate programmes. We conclude that Higher Education teachers require capability in appropriate pedagogical practice that aligns with disciplinary culture and the technologies available.
title: Qualitative Research Methods (Foundations)
Creators: name: Johanne, Düsterbeck
Publisher: name: twillo
Year: 2023
Subjects: classificationCode: 5.2
subject: FOS: Economics and business

description: Lecturers may use this syllabus as the basis for designing a class on the foundations of qualitative research methods. The lecture series is suitable for bachelor students in business administration and related fields.
A cryptochrome/photolyase class of enzymes with single-stranded DNA-specific photolyase activity
Creators: name: Sancar, A.
name: Selby, C. P.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2006

description: Photolyases and cryptochrome blue-light photoreceptors are evolutionarily related flavoproteins that perform distinct functions. Photolyases repair UV-damaged DNA in many species from bacteria to plants and animals. Cryptochromes regulate growth and development in plants and the circadian clock in animals. Recently, a new branch of the photolyase/cryptochrome family was identified. Members of this branch exhibited no or trace levels of DNA repair activity in vivo and in vitro and, therefore, were considered to be cryptochromes, and they were named cryptochrome-DASH. Here, we show that Cry-DASH proteins from bacterial, plant, and animal sources actually are photolyases with high degree of specificity for cyclobutane pyrimidine dimers in ssDNA.
School feeding in Ethiopia: a Scoping Review (protocol)
Creators: name: Meron Girma
name: Dessalegn Ararso
name: Masresha Tessema
name: Tsegaye Getachew
name: Mamuye Hadis
name: Legesse, Samson Mideksa
name: Aregash Samuel
name: Firmaye Bogale
name: Wolde, Ermias
Publisher: name: Open Science Framework
Year: 2023
Subjects: subject: Education
subject: Medicine and Health Sciences

description: This scoping study seeks to map the evidence relating to school feeding like coverage of school feeding, quality, nutritional impacts, funding issues, and its educational achievements among students in Ethiopia to inform policies, programs, future research, and offer insight related to school feeding in Ethiopia.
Supporting information for Dual stressors of infection and warming can destabilize host microbiomes
Creators: name: Li, Jingdi
name: King, Kayla
name: Stevens, Emily
name: Gao, Yunyun
Publisher: name: figshare
Year: 2023
Subjects: classificationCode: 310399
subject: Ecology not elsewhere classified

description: Supplementary Results, Figures and Tables of study "Dual stressors of infection and warming can destabilize host microbiomes".
A DNA insulator prevents repression of a targeted X-linked transgene but not its random or imprinted X inactivation
Creators: name: Smithies, O.
name: Kalantry, S.
name: Magnuson, T.
name: Ciavatta, D.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2006

description: Some genes on the inactive X chromosome escape silencing. One possible escape mechanism is that heterochromatization during X inactivation can be blocked by boundary elements. DNA insulators are candidates for blocking because they shield genes from influences of their chromosomal environment. To test whether DNA insulators can act as boundaries on the X chromosome, we inserted into the mouse X-linked Hprt locus a GFP transgene flanked with zero, one, or two copies of a prototypic vertebrate insulator from the chicken β-globin locus, chicken hypersensitive site 4, which contains CCCTC binding factor binding sites. On the active X chromosome the insulators blocked repression of the transgene, which commences during early development and persists in adults, in a copy number-dependent manner. CpG methylation of the transgene correlated inversely with expression, but the insulators on the active X chromosome were not methylated. On the inactive X chromosome, insulators did not block random or imprinted X inactivation of the transgene, and both the insulator and transgene were almost completely methylated. Thus, the chicken hypersensitive site 4 DNA insulator is sufficient to protect an X-linked gene from repression during development but not from X inactivation.
A physical linkage between cystic fibrosis airway surface dehydration and Pseudomonas aeruginosa biofilms
Creators: name: Hill, D. B.
name: Taylor, R. M.
name: Matsui, H.
name: Rogers, T. D.
name: Boucher, R. C.
name: Schwab, U. E.
name: Superfine, R.
name: Button, B.
name: Wagner, V. E.
name: Rubinstein, M.
name: Iglewski, B. H.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2006

description: A vexing problem in cystic fibrosis (CF) pathogenesis has been to explain the high prevalence of Pseudomonas aeruginosa biofilms in CF airways. We speculated that airway surface liquid (ASL) hyperabsorption generates a concentrated airway mucus that interacts with P. aeruginosa to promote biofilms. To model CF vs. normal airway infections, normal (2.5% solids) and CF-like concentrated (8% solids) mucus were prepared, placed in flat chambers, and infected with an ≈5 × 103 strain PAO1 P. aeruginosa. Although bacteria grew to 1010 cfu/ml in both mucus concentrations, macrocolony formation was detected only in the CF-like (8% solids) mucus. Biophysical and functional measurements revealed that concentrated mucus exhibited properties that restrict bacterial motility and small molecule diffusion, resulting in high local bacterial densities with high autoinducer concentrations. These properties also rendered secondary forms of antimicrobial defense, e.g., lactoferrin, ineffective in preventing biofilm formation in a CF-like mucus environment. These data link airway surface liquid hyperabsorption to the high incidence of P. aeruginosa biofilms in CF via changes in the hydration-dependent physical–chemical properties of mucus and suggest that the thickened mucus gel model will be useful to develop therapies of P. aeruginosa biofilms in CF airways.
Novel seismic probes: The magnetic field measurement in red giants and the Sun
Creators: name: Takata, Masao
Publisher: name: Zenodo
Year: 2024

description: One of the most successful novel probes in asteroseismology in recent years is the one to measure the internal magnetic field in red giants from their frequencies. The result has a significant impact in not only asteroseismology, but also stellar physics in general. On the other hand, it was once claimed in helioseismology that the magnetic field in the subsurface layer is responsible for the change in oscillation frequencies along with the activity of the Sun. However, the situation then became complicated because the predicted change in the field strength turned out to be much larger than actually observed at the photosphere. The purpose of this presentation is to make it clear why the two cases, one for red giants and the other for the Sun, are so different from each other.
Kooperationsmodelle zur Ertüchtigung von OSS zwischen OSS-Entwicklern/-Unternehmen und Anwender-Communities
Creators: name: Michler, Jonas
Publisher: name: Zenodo
Year: 2023
Subjects: Open Source
OSS
Softwarelösungen
Governancemodelle
Julius-Maximilians-Universität

description: In dieser Arbeit wird darauf eingegangen, welche Voraussetzungen geschaffen sein müssen, um eine Kooperation zwischen Entwicklern und Anwendern von OSS zu ermöglichen, damit deren Weiterentwicklung ermöglicht wird. Darüber hinaus soll anhand dieser Voraussetzungen relevante Software in universitärer Nutzung beurteilt und bewertet werden, um eine Perspektive zu bieten, in welchen Bereichen eine tiefere Zusammenarbeit möglich ist.
Heavy-ion physics studies for the Future Circular Collider
Creators: name: Armesto, Nestor
name: Dainese, Andrea
name: d'Enterria, David
name: Masciocchi, Silvia
name: Roland, Christof
name: Salgado, Carlos
name: van Leeuwen, Marco
name: Wiedemann, Urs
Publisher: name: arXiv
Year: 2014
Subjects: subject: Nuclear Experiment (nucl-ex)
subject: High Energy Physics - Phenomenology (hep-ph)
subject: Nuclear Theory (nucl-th)
subject: FOS: Physical sciences

description: The Future Circular Collider (FCC) design study is aimed at assessing the physics potential and the technical feasibility of a new collider with centre-of-mass energies, in the hadron-hadron collision mode including proton and nucleus beams, more than seven-times larger than the nominal LHC energies. An electron-positron collider in the same tunnel is also considered as an intermediate step, which would provide the electron-hadron option in the long term. First ideas on the physics opportunities with heavy ions at the FCC are presented, covering the physics of Quark-Gluon Plasma, gluon saturation, photon-induced collisions, as well as connections with ultra-high-energy cosmic rays.
description: 4 pages, 3 figures, proceedings of Quark Matter 2014, Darmstadt, Germany, submitted to Nucl. Phys. A
A structural model reveals energy transduction in dynein
Creators: name: Chen, Y.
name: Elston, T. C.
name: Dokholyan, N. V.
name: Serohijos, A. W. R.
name: Ding, F.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2006

description: Intracellular active transport is driven by ATP-hydrolyzing motor proteins that move along cytoskeletal filaments. In particular, the microtubule-associated dynein motor is involved in the transport of organelles and vesicles, the maintenance of the Golgi, and mitosis. However, unlike kinesin and myosin, the mechanism by which dynein converts chemical energy into mechanical force remains largely a mystery, due primarily to the lack of a high-resolution molecular structure. Using homology modeling and normal mode analysis, we propose a complete atomic structure and a mechanism for force generation by the motor protein dynein. In agreement with very recent electron microscopy (EM) reconstructions showing dynein as a ring-shaped heptamer, our model consists of six ATPases of the AAA (ATPases associated with various cellular activities) superfamily and a C-terminal domain, which is experimentally known to control motor function. Our model shows a coiled coil spanning the diameter of the motor that accounts for previously unidentified structures in EM studies and provides a potential mechanism for long-range communication between the AAA domains. Furthermore, normal mode analysis reveals that the subunits of the motor that contain the nucleotide binding sites exhibit minimal movement, whereas the rest of the motor is very mobile. Our analysis suggests the likely domain rearrangements of the motor unit that generate its power stroke. This study provides insights into the structure and function of dynein that can guide further experimental investigations into energy transduction in dynein.