title: ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ ÑÐ¾ÑÑÐ°Ð²Ð° Ð¼Ð¸ÐºÑÐ¾Ð±Ð¸Ð¾Ð¼Ð° Ð»Ð¸ÑÑÑÐµÐ² Ð¸ ÐºÐ¾ÑÐ½ÐµÐ¹ Stevia rebaudiana Bertoni Ð¿ÑÐ¸ ÑÐ¾ÑÑÐµ Ð½Ð° Ð±ÐµÑÐ¿Ð¾ÑÐ²ÐµÐ½Ð½ÑÑ ÑÑÐ±ÑÑÑÐ°ÑÐ°Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Study of the microbiome composition of the leaves and roots of Stevia rebaudiana Bertoni when growing on baseless substrates
Creators: name: Ð¯ÐºÑÑÐ¸Ð½Ð°, ÐÐ¸ÐºÑÐ¾ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: ÐÐ°ÐºÑÐµÑÐ¸Ð¸
ÐÐ¸Ð¾ÑÐµÑ Ð½Ð¾Ð»Ð¾Ð³Ð¸Ñ
ÑÐºÑÑÑÐ°ÐºÑ ÑÑÐµÐ²Ð¸Ð¸
ÑÑÐµÐ²Ð¸Ð¾Ð»Ð³Ð»Ð¸ÐºÐ¾Ð·Ð¸Ð´
Ð¼Ð¸ÐºÑÐ¾Ð±Ð¸Ð¾Ð¼ ÑÑÐµÐ²Ð¸Ð¸
stevia extract
steviolglycoside
stevia microbiome

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐ¾ÑÑÐ°Ð²Ð° Ð¼Ð¸ÐºÑÐ¾Ð±Ð¸Ð¾Ð¼Ð° Ð»Ð¸ÑÑÑÐµÐ² Ð¸ ÐºÐ¾ÑÐ½ÐµÐ¹ Stevia rebaudiana Bertoni Ð¿ÑÐ¸ ÑÐ¾ÑÑÐµ Ð½Ð° Ð±ÐµÑÐ¿Ð¾ÑÐ²ÐµÐ½Ð½ÑÑ ÑÑÐ±ÑÑÑÐ°ÑÐ°Ñ. ÐÐ°Ð´Ð°ÑÐ¸: 1. ÐÐ¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð¾ ÐºÐ¾Ð»Ð¾Ð½Ð¸ÐµÐ¾Ð±ÑÐ°Ð·ÑÑÑÐ¸Ñ ÐµÐ´Ð¸Ð½Ð¸Ñ ÑÐ½Ð´Ð¾ÑÐ¸ÑÐ½ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ Ð² Ð»Ð¸ÑÑÐµ ÑÑÐµÐ²Ð¸Ð¸ Ð¿Ð¾ÑÐ»Ðµ ÑÑÐµÑÐ¸Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð¿Ð¾Ð²ÐµÑÑÐ½Ð¾ÑÑÐ¸ Ð³Ð¸Ð¿Ð¾ÑÐ»Ð¾ÑÐ¸ÑÐ¾Ð¼ Ð½Ð°ÑÑÐ¸Ñ; 2. ÐÐ¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð¾ Ð°ÐºÑÐ¸Ð½Ð¾Ð¼Ð¸ÑÐµÑÐ¾Ð², Ð³ÑÐ¸Ð±Ð¾Ð² Ð¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹, Ð°ÑÑÐ¾ÑÐ¸Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ Ñ ÐºÐ¾ÑÐ½ÑÐ¼Ð¸ ÑÑÐµÐ²Ð¸Ð¸, Ð²ÑÑÐ°ÑÐµÐ½Ð½Ð¾Ð¹ Ð½Ð° ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ Ð±ÐµÑÐ¿Ð¾ÑÐ²ÐµÐ½Ð½ÑÑ ÑÑÐ±ÑÑÑÐ°ÑÐ°Ñ; 3. ÐÐ¾Ð»ÑÑÐ¸ÑÑ ÑÐ¸ÑÑÑÐµ ÐºÑÐ»ÑÑÑÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹, Ð°ÑÑÐ¾ÑÐ¸Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ ÑÐ¾ ÑÑÐµÐ²Ð¸ÐµÐ¹, Ð¸ Ð¾Ð¿Ð¸ÑÐ°ÑÑ Ð¸Ñ ÐºÑÐ»ÑÑÑÑÐ°Ð»ÑÐ½ÑÐµ Ð¸ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð°; 4. ÐÐ¾Ð´Ð¾Ð±ÑÐ°ÑÑ Ð¾Ð¿ÑÐ¸Ð¼Ð°Ð»ÑÐ½ÑÐ¹ ÑÐ¾ÑÑÐ°Ð² Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ´Ñ Ð´Ð»Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ ÐÑÐµÐ´Ð¼ÐµÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð¼Ð¸ÐºÑÐ¾Ð±Ð½ÑÑ ÑÐ¾Ð¾Ð±ÑÐµÑÑÐ² ÐºÐ¾ÑÐ½ÐµÐ¹ Ð¸ Ð»Ð¸-ÑÑÑÐµÐ² ÑÑÐµÐ²Ð¸Ð¸ Ð² Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾ÑÑÐ¸ Ð¾Ñ ÑÑÐ±ÑÑÑÐ°ÑÐ° Ð²ÑÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ñ ÑÐ°ÑÑÐµÐ½Ð¸Ñ. ÐÐ¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ ÑÐ½Ð´Ð¾ÑÐ¸ÑÐ½Ð°Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐ»Ð¾ÑÐ° Ð»Ð¸ÑÑÑÐµÐ² ÑÑÐµÐ²Ð¸Ð¸ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÐµÐ½Ð° Ð±Ð°ÐºÑÐµÑÐ¸ÑÐ¼Ð¸ Ð¸ ÑÐ¾ÑÑÐ°Ð²Ð»ÑÐµÑ (2,34 Â± 0,26) * 10^6 ÐÐÐ/Ð³ ÑÑÑÐ¾Ð³Ð¾ Ð»Ð¸ÑÑÐ°. ÐÑÑÐ¾ÑÐ¸Ð°ÑÐ¸Ð²Ð½Ð°Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐ»Ð¾ÑÑ ÐºÐ¾ÑÐ½ÐµÐ¹ ÑÑÐµÐ²Ð¸Ð¸, Ð·Ð°Ð²Ð¸ÑÐ¸Ñ Ð¾Ñ ÑÐ¾Ð³Ð¾, Ð² ÐºÐ°ÐºÐ¾Ð¼ ÑÑÐ±ÑÑÑÐ°ÑÐµ Ð±ÑÐ»Ð¸ Ð²Ñ-ÑÐ°ÑÐµÐ½Ñ ÑÐ°ÑÑÐµÐ½Ð¸Ñ. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ 5 ÑÐ¸ÑÑÑÑ ÐºÑÐ»ÑÑÑÑ ÑÐ½Ð´Ð¾ÑÐ¸ÑÐ½ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ Ð¸ 7 Ð°ÑÑÐ¾ÑÐ¸Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ Ñ ÐºÐ¾ÑÐ½ÑÐ¼Ð¸ ÑÑÐµÐ²Ð¸Ð¸ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ñ ÑÐ°ÑÑÐ¸ Ð½Ð° Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ´ÐµÂ  Ñ Ð²Ð¾Ð´Ð½ÑÐ¼ ÑÐºÑÑÑÐ°ÐºÑÐ¾Ð¼ ÑÑÐ¾Ð³Ð¾ ÑÐ°ÑÑÐµÐ½Ð¸Ñ. ÐÐ¾ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ð¼ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð°Ð¼Â  Ð±Ð¾Ð»ÑÑÐ¸Ð½ÑÑÐ²Ð¾ ÐºÑÐ»ÑÑÑÑ ÑÐ²Ð»ÑÑÑÑÑ ÐºÐ¾ÐºÐºÐ°Ð¼Ð¸, Ð¸ÑÐºÐ»ÑÑÐµÐ½Ð¸Ðµ ÑÐ¾ÑÑÐ°Ð²Ð»ÑÑÑ ÐºÑÐ»ÑÑÑÑÑ 6, 7 Ð¸ 12. ÐÑÐ´ÐµÐ»ÐµÐ½Ð½ÑÐµ Ð°ÑÑÐ¾ÑÐ¸Ð°ÑÐ¸Ð²Ð½ÑÐµ Ð±Ð°ÐºÑÐµÑÐ¸Ð¸ ÐºÐ¾ÑÐ½ÐµÐ¹ ÑÑÐµÐ²Ð¸Ð¸ Ð¸ ÑÐ½Ð´Ð¾ÑÐ¸ÑÐ½ÑÐµ Ð±Ð°ÐºÑÐµÑÐ¸Ð¸ Ð»Ð¸ÑÑÑÐµÐ² Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»ÑÐ½Ð¾ Ð¼Ð¾Ð³ÑÑ Ð±ÑÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ Ð´Ð»Ñ ÑÐ¾Ð·Ð´Ð°Ð½Ð¸Ñ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÑ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð², Ð¿ÑÐµÐ´Ð½Ð°Ð·Ð½Ð°ÑÐµÐ½Ð½ÑÑ Ð´Ð»Ñ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÐ¸ ÑÑÐµÐ²Ð¸Ð¸ Ñ ÑÐµÐ»ÑÑ Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð½Ð° ÑÐ¾ÑÑ ÑÑÐ¾Ð³Ð¾ ÑÐ°ÑÑÐµÐ½Ð¸Ñ Ð¸ ÑÐ¸Ð½ÑÐµÐ· ÑÑÐµÐ²Ð¸Ð¾Ð»Ð³Ð»Ð¸ÐºÐ¾Ð·Ð¸Ð´Ð¾Ð². Ð Ð°Ð±Ð¾ÑÐ° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ ÑÐµÐºÑÐ¾ÑÐ° ÑÐºÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð¼Ð¸ÐºÑÐ¾Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ð¸ ÐÐ³ÑÐ¾-ÑÐ¸Ð·Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð½Ð°ÑÑÐ½Ð¾-Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÑÐºÐ¾Ð³Ð¾ Ð¸Ð½ÑÑÐ¸ÑÑÑÐ°. ÐÐ»Ñ Ð½Ð°Ð¿Ð¸ÑÐ°Ð½Ð¸Ñ Ð°Ð½Ð°Ð»Ð¸ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¾Ð±Ð·Ð¾ÑÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ ÑÐ¾Ð²ÑÐµÐ¼ÐµÐ½Ð½ÑÐµ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ: Elsevier ScienceDirect, PubMed, Google ÐÐºÐ°Ð´ÐµÐ¼Ð¸Ñ, eLIBRARY.RU. ÐÐ»Ñ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÐ¸ Ð´Ð°Ð½Ð½ÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ ÑÐ¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ðµ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ð½Ð¾Ðµ Ð¾Ð±ÐµÑÐ¿ÐµÑÐµÐ½Ð¸Ðµ GraphPad Prism Ð¸ Microsoft Excel.
description: This work is devoted to the study of the microbiome composition of the leaves and roots of Stevia rebaudiana Bertoni when growing on baseless substrates. Tasks: 1. To determine the number of colony-forming units of endophytic bacteria in a stevia leaf after sterilization of the surface with sodium hypochlorite; 2. Determine the number of actinomycetes, fungi and bacteria associated with stevia roots grown on various groundless substrates; 3. To obtain pure cultures of bacteria associated with stevia and describe their cultural and morphological properties; 4. To select the optimal composition of the nutrient medium for the cultivation of isolated bacteria The subject of the study is the changes in microbial communities of stevia roots and leaves depending on the substrate of plant cultivation. It has been shown that the endophytic microflora of stevia leaves is represent-ed by bacteria and amounts to (2.34 Â± 0.26) * 10^6 CFU/g of dry leaf. The associative microflora of stevia roots depends on the substrate in which the plants were grown. The obtained 5 pure cultures of endophytic bacteria and 7 associated with stevia roots are able to grow on a nutrient medium with an aqueous extract of this plant. According to morphological properties, most crops are cocci, with the exception of crops 6, 7 and 12. The isolated associative bacteria of stevia roots and endophytic bacteria of leaves can potentially be used to create bacterial preparations intended for the treatment of stevia in order to study the effect on the growth of this plant and the synthesis of steviolglycosides. The work was carried out on the basis of the environmental microbiology sec-tor of the Agrophysical Research Institute. To write an analytical review, we used modern databases: Elsevier ScienceDirect, PubMed, Google Academy, eLIBRARY.RU. Modern GraphPad Prism and Microsoft Excel software were used for data processing.