cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Development of enhanced buccal films with Actinidia arguta fruit extract for oral mucositis prevention: from in vitro buccal models to ex vivo investigations
Creators: name: Teixeira, Filipa
name: Silva, Ana Margarida
name: Macedo, Catarina
name: Estevinho, Berta
name: Sut, Stefania
name: Dall’Acqua, Stefano
name: Delerue-Matos, Cristina
name: Costa, Paulo C.
name: Rodrigues, Francisca
Publisher: name: Scientific Letters
Year: 2024

description: Background: Oral mucositis (OM) is a common side effect of cancer treatments such as chemotherapy [1], being characterized by disruption of the oral mucosa integrity, inflammation, and pain [1,2]. The treatment strategies to prevent and treat OM are still unsatisfactory, leading to the search of new active compounds, particularly from natural sources, such as Actinidia arguta fruits [1]. A. arguta fruit, commonly known as kiwiberry, has been associated with different therapeutic properties and pro-healthy benefits, particularly antioxidant, anti-inflammatory and anticancer effects [3,4], due to the fruit’s outstanding content in phenolic compounds, vitamins, and organic acids [3,4]. Objective: The aim of this study was to develop buccal films with A. arguta fruit extract as active ingredient to prevent OM symptoms. Methods: The films were prepared by solvent casting employing 1% of HPMC K100 LV EP, 2.5% glycerin, and A. arguta extract as solvent, previously prepared by Ultrasound-Assisted Extraction [4]. Results: Different films parameters were assessed, namely physical features (weight: 194.8 mg; thickness: 0.37 mm; disintegration time: 15.05 min; moisture content: 10.53%; swelling capacity: 55.95%), mechanical properties (resistance to extension: 10.11 N; percent of elongation: 36.10%; Young’s modulus: 0.0034 MPa) and antioxidant/antiradical activities (TPC = 6.46 mg GAE/g film; FRAP = 49.45 µmol FSE/g film; ABTS = 3.74 mg AAE/g film; DPPH = 4.90 mg TE/g film). In vitro cell assays attested the absence of negative effects on HSC-3 and TR146 oral cell lines. Most important, the compounds release profile was assessed through in vitro cell models (TR146) and ex vivo assay with porcine mucosa coupled to LC/DAD-ESI-MS quantification. The results revealed high permeation of rutin (88.32%), quercetin-3-O-glucoside (84.95%) and catechin (79.74%). Conclusions: Overall, these results highlight the significant potential and safety of buccal films with A. arguta fruit extract to prevent OM condition.
description: Scientific Letters, Vol. 1 No. Sup 1 (2024)
Spilomyia saltuum
Creators: name: Kočić, Anja
name: Vujić, Ante
name: Tot, Tamara
name: Milosavljević, Marina Janković
name: Groot, Maarten De
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Syrphidae
Spilomyia
Spilomyia saltuum

description: Spilomyia saltuum (Fabricius, 1794).
Regions: GO, CL, DR.
Reference: Strobl (1894).
description: Published as part of Kočić, Anja, Vujić, Ante, Tot, Tamara, Milosavljević, Marina Janković & Groot, Maarten De, 2023, An updated checklist of the hoverflies (Diptera: Syrphidae) of Slovenia, pp. 189-227 in Zootaxa 5297 (2) on page 206, DOI: 10.11646/zootaxa.5297.2.2, http://zenodo.org/record/7993180
Dirac-Bergmann analysis and Degrees of Freedom of Coincident $f(Q)$-gravity
Creators: name: Tomonari, Kyosuke
name: Bahamonde, Sebastian
Publisher: name: arXiv
Year: 2023
Subjects: subject: General Relativity and Quantum Cosmology (gr-qc)
subject: High Energy Physics - Theory (hep-th)
subject: FOS: Physical sciences

description: We investigate the propagating degrees of freedom of $f(Q)$-gravity in a $4$-dimensional space-time under the imposition of the coincident gauge by performing the Dirac-Bergmann analysis. In this work, we start with a top-down reconstruction of the metric-affine gauge theory of gravity based only on the concept of a vector bundle. Then, the so-called geometrical trinity of gravity is introduced and the role of the coincident GR is clarified. After that, we reveal relationships between the boundary terms in the variational principle and the symplectic structure of the theory in order to confirm the validity of the analysis for our studied theories. Then, as examples, we revisit the analysis of GR and its $f(\lc{R})$-extensions. Finally, after reviewing the Dirac-Bergmann analysis of the coincident GR and that of $f(T)$-gravity, we perform the analysis of coincident $f(Q)$-gravity. Under the imposition of appropriate spatial boundary conditions, we find that, as a generic case, the theory has five primary, three secondary, and two tertiary constraint densities and all these constraint densities are classified into second-class constraint density; the number six is the propagating degrees of freedom of the theory and there are no longer any remaining gauge degrees of freedom. We also discuss the condition of providing seven pDoF as a generic case. The violation of diffeomorphism invariance of coincident $f(Q)$-gravity make it possible to emerge such several sectors.
description: 26 pages. v2: Added references and footnotes, revised overall, for submission. 26 pages. v3: Added new Sec. V-A and references, revised overall. 31 pages. v4: Published in EPJC (Single column version)
title: Scor/Ioc Working Group 119: 'Quantitative Ecosystem Indicators For Fisheries Management'
Creators: name: Philippe, Cury
name: Christensen, Villy
Publisher: name: ASC 2001 - T - Theme session
Year: 2024
Subjects: Technologies and data
Ecosystem observation, processes and dynamics

description: No abstracts are to be cited without prior reference to the author.The definition of quantitative indicators for marine ecosystems from an environmental, ecological and fisheries perspective could provide a comprehensive bridge between the different scientific disciplines themselves, but also could constitute an efficient way to communicate those results for management purposes. The exploitation of renewable resources must respect marine diversity and ecosystems functioning, and we must direct our efforts, as scientists, toward reconciling long-term environmental, ecological, economical and social objectives and short-term constraints. In order to establish an international network of scientists interested in developing ecosystem indicators in different fields and disciplines for the marine environment an initiative has recently been launched through a joint SCOR/IOC Working Group 119 on 'Quantitative Ecosystem Indicators for Fisheries Management'. Its general objective is to develop theory to evaluate changes in marine ecosystems (both states and processes) from environmental, ecological and fisheries perspectives. It is the intention during a period of four years (2001-2004) to define generic indicators that can be used for marine ecosystems and to apply them to a variety of ecosystems in order to evaluate their usefulness. This is expected to lead to new and refreshing insights, which will be essential for defining and evaluating new ecosystem management goals.
title: The Ibts Database: A Plea For Quality Control
Creators: name: Daan, Niels
Publisher: name: ASC 2001 - T - Theme session
Year: 2024
Subjects: Technologies and data
Ecosystem observation, processes and dynamics

description: No abstracts are to be cited without prior reference to the author.The IBTS database as maintained at ICES Headquarters has been submitted to a quality check with reference to reported size distributions and species identifications. The check is based largely on a comparison of reported size distributions with available information on maximum length in the literature and on consistency in species compositions reported by individual countries (and sometimes years and areas). The results are alarming. For a large number of species the database contains unrealistic information. In many cases errors may be tracked to simple input errors, but clearly there have also been, and still are, severe species identification problems among all countries participating in the survey. Misidentifications involve errors in abundance of at least two species and, based on the limited analysis presented, a conservative guess is that at least 30% of all species reported may be affected. This not only means that trends in abundance of individual species may be biased, but, more importantly, it reduces the value of the IBTS database for diversity studies of the North Sea fish community to virtually zero. I present a strong plea for a comprehensive check of all data in the existing database, with an appropriate correction of uncertain identifications to higher taxa to reflect inherent uncertainties, and to embark on a serious quality control programme to ensure that future surveys yield only reliable information at the species level. The scientific credibility of ICES is at stake, because it is a waste to maintain a flawed database at ICES Headquarters.
Please See Other One Called "An Elementary Proof of Goldbach's Conjecture", thats Correct. https://figshare.com/articles/preprint/An_Elementary_Proof_of_Golbach_Conjecture/18737363Five Elementary Proofs of Goldbach Conjecture are only the first sketches of the final result: "An Elementary Proof of Goldbach's Conjecture".
Creators: name: Chavez, Danilo
Publisher: name: figshare
Year: 2023
Subjects: classificationCode: 490401
subject: Algebra and number theory
classificationCode: 490501
subject: Applied statistics
classificationCode: 490506
subject: Probability theory

description: In this present paper we will show you five elementary proofs of the Goldbach Conjecture based on probabilities.
title: Cornelius L. Moore:  A Northern Soldier's Perspective on the Civil War
Creators: name: Crew Jr., Roger T.
Publisher: name: Old Dominion University
publisherIdentifier: https://ror.org/04zjtrb98
publisherIdentifierScheme: ROR
Year: 1980

description: This is a study of the Civil War through the letters of Cornelius L. Moore, a New York soldier in the Army of the Potomac. His letters span the years between August 1861- August 1864 and reveal a typical Union soldier's attitudes and experiences. Moore discussed such topics as the draft, the use of black soldiers, army leadership, and soldier life. His insight and frankness about these and other subjects make this story more than simply another account of a Civil War participant.
Syritta pipiens
Creators: name: Kočić, Anja
name: Vujić, Ante
name: Tot, Tamara
name: Milosavljević, Marina Janković
name: Groot, Maarten De
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Syrphidae
Syritta
Syritta pipiens

description: Syritta pipiens (Linnaeus, 1758).
Regions: UC, GO, SS, CK, CL, DR, MU, CS, LC, SA.
Reference: Scopoli (1763) (as Conops pipiens), Strobl (1893b, 1910), Glumac (1956), Leclercq (1961), Lambeck (1968), de Groot (2004), de Groot & Govedič (2008), Van Steenis et al. (2013).
description: Published as part of Kočić, Anja, Vujić, Ante, Tot, Tamara, Milosavljević, Marina Janković & Groot, Maarten De, 2023, An updated checklist of the hoverflies (Diptera: Syrphidae) of Slovenia, pp. 189-227 in Zootaxa 5297 (2) on page 206, DOI: 10.11646/zootaxa.5297.2.2, http://zenodo.org/record/7993180
Spin Rotations in a Bose-Einstein Condensate Driven by Counterflow and Spin-independent Interactions
Creators: name: Spierings, David C.
name: Thywissen, Joseph H.
name: Steinberg, Aephraim M.
Publisher: name: arXiv
Year: 2023
Subjects: subject: Quantum Gases (cond-mat.quant-gas)
subject: Quantum Physics (quant-ph)
subject: FOS: Physical sciences

description: We observe spin rotations caused by atomic collisions in a non-equilibrium Bose-condensed gas of $^{87}$Rb. Reflection from a pseudomagnetic barrier creates counterflow in which forward- and backward-propagating matter waves have partly transverse spin directions. Even though inter-atomic interaction strengths are state-independent, the indistinguishability of parallel spins leads to spin dynamics. A local magnetodynamic model, which captures the salient features of the observed spin textures, highlights an essential connection between four-wave mixing and collisional spin rotation. The observed phenomenon has previously been thought to exist only in nondegenerate gases; our observations and model clarify the nature of these effective-magnetic spin rotations.
description: 13 pages, 7 figures
The unseen ingredients: a systematic review of the prevalence of microplastics and nanoplastics in our food
Creators: name: Mota, Carolina
name: Martins, Zita E.
name: Ramos, Helena
name: M. P. L. V. O. Ferreira, Isabel
name: Carvalho, Márcia
name: Araújo, Ana Margarida
Publisher: name: Scientific Letters
Year: 2024

description: Background:
 Plastics have revolutionized every aspect of modern life [1, 2], offering convenience but also bringing forth environmental and health hazards. Their exponential production and improper disposal have led to the global burden of microplastics (MPs) and nanoplastics (NPs) in the environment [3, 4]. These tiny particles possess the alarming ability to infiltrate the food chain, thereby posing a potential threat to human health [1-3, 5]. Objective: This study aims to evaluate the prevalence of MPs/NPs contamination in food and beverages, emphasizing the urgency of addressing this invisible threat and providing future directions. Methods: Employing a PRISMA methodology, a comprehensive literature review was conducted on PubMed, Scopus, and Web of Science until August 15, 2023. From an initial pool of 4078 potentially relevant studies, duplicates and unrelated works were removed, and 229 articles focusing on edible products were selected for analysis, resulting in 1630 data points. Systematic categorization included food types, detection techniques, particle characteristics, and polymer compositions. Results: Our investigation unveiled that over 95% of the examined food items were found to be contaminated with MPs/NPs. Predominant detection methods included FTIR spectroscopy and microscopy, uncovering fragments and fibers in a spectrum of colors such as blue, black, red, transparent, and white. Polypropylene, polyethylene, and PET emerged as the primary polymers present across diverse food categories. Notably, fruits and vegetables exhibited the highest contamination rates (126150 items/g), followed by sauces, beverages, and dairy products (45 to 8 items/L). Conversely, seafood, sweeteners, canned foods, salts, meats (0.7 up to 0.014 items/g), rice (56 µg/g), and soy-based products (0 µg/g) displayed comparatively lower contamination levels. Conclusions: This study highlights significant gaps in our knowledge regarding the extent of MPs/NPs contamination in our diet. Future research is imperative to expand our understanding of their presence, particularly in under- or unexplored foods categories such as fruits and vegetables, dairy products, meat, bakery products, baby foods, and beverages other than water. This issue cannot be underestimated, as it pertains directly to safeguarding human health in the face of an unseen but potentially pervasive threat.
description: Scientific Letters, Vol. 1 No. Sup 1 (2024)
Drug use among the student population of the University of Porto: Analysis of prevalence patterns
Creators: name: Garcia, Helena Rafaela
name: Roque-Bravo, Rita
name: Dias-Da-Silva, Diana
Publisher: name: Scientific Letters
Year: 2024

description: Background: Alcohol, tobacco, and illicit drugs use by university students is a complex issue that has garnered concern and interest from public health officials, educators, and scientific community [1]. This concern is driven by various factors, including the potential to affect academic performance and mental and physical health. Objective: This study was designed to determine the prevalence and patterns of psychotropic drug use among students at the University of Porto (UP). Methods: This was an observational, transversal, and descriptive study approved by the Ethics Committee of CHUP/ICBAS (2021/CE/P006[P346/CETI/ICBAS]). Voluntary participants (n=4,052) from the student body of UP enrolled in the academic year 2021/2022 completed a web-based questionnaire assessing self-reported drug use across a range of specified psychoactive substances, between February and April of 2022. Gender, age, living situation, economic status, drug use patterns and intent were also collected and used as stratification and association variables. Results: The three most consumed substances were alcohol, tobacco, and cannabis and its derivatives (CAD). Notably, 23.0% of respondents declared smoking cigarettes every day, while only 1.8% of alcohol users drank every day (36.8% drank it 2-4 times per month). “Nightclubs and bars” and “other people’s homes” were the most cited locations for alcohol and cigarette use. Illicit drugs have more variety when it comes to settings depending on their usual context and motive for use. "Have fun" was reported by half of the students as the reason for drinking alcohol, while curiosity was the main drive for smoking tobacco and CAD. Conclusions: Students have access to and consume different (il)licit drugs. These results confirm the importance of this type of research, tracing a more defined image of the drug use prevalence patterns of this population, which in turn can aid health officials and other institutions when constructing and enforcing population-specific prevention and drug treatment measures.
description: Scientific Letters, Vol. 1 No. Sup 1 (2024)
Pore-Space Alteration Induced by Brine Acidification in Subsurface Geologic Formations
Creators: name: Ovaysi, Saeed
name: Piri, Mohammad
Publisher: name: University of Wyoming. Libraries
Year: 2014
Subjects: carbon sequestration
pore-scale modeling
reactive transport
Engineering

description: A new Lagrangian particle-based method is presented to simulate reactive transport in natural porous media. This technique is based on Modified Moving Particle Semi-implicit (MMPS) and takes as input high-resolution voxel images of natural porous media. The flow field in the medium is computed by solving the incompressible Navier-Stokes equations. Moreover, a multicomponent ion transport model is coupled with a homogeneous and heterogeneous reactions module to handle pore-space alteration (i.e., pore-wall dissolution). The model is first successfully validated against the experimental data available in the literature. Subsequently, X-ray microtomographic images of two naturally occurring porous media are used to investigate the impact of reaction kinetics and pore-space topology on pore-space alteration induced by brine acidification in subsurface conditions. We observed that at the normal rates of reactions no significant change in porosity and permeability takes place in the short term. Whereas, higher reaction rates caused major changes in the macroscopic properties (e.g., porosity and permeability) of the rocks. We also show that these changes are strongly affected by the rocks' pore-scale topologies. Key points: A direct pore-scale model is developed to simulate rock/fluid chemical reactions Chemical alteration of pore space leads to significant changes in permeability Pore-space topology determines the extent of change in permeability and porosity.
Tramadol and its main metabolites: toxicological effects on zebrafish embryos and larvae
Creators: name: Rodrigues, Pedro
name: Oliva-Teles, Luís
name: Carvalho, António Paulo
name: Guimarães, Laura
Publisher: name: Scientific Letters
Year: 2024

description: Background: Tramadol (TRA) and its main metabolites are among the most detected pharmaceutical compounds in aquatic ecosystems, reaching concentrations in the µg/L range. In humans, TRA acts on different receptors of the noradrenergic, serotoninergic, dopaminergic and opioid systems. Also, it is metabolized into the active metabolite o-desmethyltramadol (OTRA) and inactive metabolite n-desmethyltramadol (NTRA). In fish, TRA was reported to have several toxic effects [1,2]. However, for OTRA and NTRA there is no information about their toxicity in fish. Objective:the main aims of this work were to investigate and compare the effects of TRA, OTRA and NTRA on zebrafish embryonic development; behavioural responses; expression profiles of genes related to the monoaminergic and detoxification systems and proteome, as well as develop and initial draft of an adverse outcome pathway (AOP) Methods: Zebrafish embryos were exposed (0.1−100µg/L) for 168hpf to TRA, OTRA and NTRA [3]. During the exposure, several developmentalmalformations were registered. Behaviour was evaluated through a sensorimotor assay. Gene expression for 32 target genes was obtained through qPCR, while shotgun proteomics protocols were employed for the proteome evaluation. Results:TRA and OTRA were found to cause significant increases in embryonic anomalies, namely in the yolk sac, spine, and otoliths, at the highest concentrations. Decreased sensorimotor responses were observed for exposure to 0.1 and 100µg/L of TRA and OTRA. Different gene expression profiles were found between TRA and the metabolites OTRA and NTRA, with a predominance of non-monotonic responses. Shotgun proteomics indicates that TRA and OTRA impact pathways linked to vital biological processes. An initial drat AOP was performed.  Conclusions: The obtained results indicated that exposure to TRA, OTRA and NTRA can negatively impact aquatic non-target species at different levels of biological organization. These results also raise awareness for the inclusion of pharmaceutical in monitoring programmes.
description: Scientific Letters, Vol. 1 No. Sup 1 (2024)
Temnostoma bombylans
Creators: name: Kočić, Anja
name: Vujić, Ante
name: Tot, Tamara
name: Milosavljević, Marina Janković
name: Groot, Maarten De
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Syrphidae
Temnostoma
Temnostoma bombylans

description: Temnostoma bombylans (Fabricius, 1805).
Regions: UC, GO, SS, CA, CL, LC, SA.
Reference: Lambeck (1968), Glumac (1972), de Groot (2004), de Groot & Govedič (2008), Van Steenis et al. (2013).
description: Published as part of Kočić, Anja, Vujić, Ante, Tot, Tamara, Milosavljević, Marina Janković & Groot, Maarten De, 2023, An updated checklist of the hoverflies (Diptera: Syrphidae) of Slovenia, pp. 189-227 in Zootaxa 5297 (2) on page 206, DOI: 10.11646/zootaxa.5297.2.2, http://zenodo.org/record/7993180
The effect of synthetic cannabinoid ADB-FUBINACA on primary neuronal cultures ß-galactosidase activity: preliminary findings
Creators: name: Roque-Bravo, Rita
name: Carmo, Helena
name: Silva, João Pedro
name: Carvalho, Félix
name: Dias-Da-Silva, Diana
Publisher: name: Scientific Letters
Year: 2024

description: Background: ADB-FUBINACA (ADB-FUB) is a synthetic cannabinoid (SC) that has gained popularity among users as a new psychoactive substance. This stems from SC's pharmacological similarity to the active principle of cannabis, D9-tetrahydrocannabinol (THC). However, unlike THC, SCs demonstrate full agonism of cannabinoid receptors 1 and 2 [1]. Recent scientific developments have shown that cannabis use may aggravate ageing-related parameters [2,3]. Moreover, a study using human fibroblasts revealed that 1 µM THC (2h-long exposure, for 15 days) can increase ß-galactosidase activity [3], which serves as a first-line marker for cellular senescence. Objective: This study was designed to investigate whether these biologically-relevant concentrations could accelerate neuronal ageing. Methods: PHC were isolated from Wistar rat day 18-19 embryos and cultured for up to 21 days-in-vitro (DIV). Exposure to 1 pM, 1 nM and 1 µM ADB-FUB (concentrations previously shown to be non-cytotoxic to PHC) started either on DIV3 or DIV7 and was maintained up to 21 DIV. At that final timepoint, ß-galactosidase activity was evaluated. DMSO at 0.02% was employed as solvent control. Results: Under these experimental conditions, PHC exposed to 1 nM and 1 µM ADB-FUB in the DIV3-21 protocol had lower ß-galactosidase activity when compared to control conditions (p<0.05, 1 nM; p<0.001, 1 µM). No statistically significant results were registered for PHC under the DIV7-21 exposure protocol. Conclusions: These findings are, to the best of our knowledge, the first evidence of a potential “anti-ageing” effect of ADB-FUB. Evaluation of other senescence-related endpoints will follow. Moreover, experiments using another in vitro neuronal model (human neuroblastoma cell line SH-SY5Y) are underway to compare the effects of the same drug in different models and further substantiate conclusions on ADB-FUB’s effect.
description: Scientific Letters, Vol. 1 No. Sup 1 (2024)
Enantiomeric biodistribution and toxicity of 3-chloromethcathinone (3-CMC) in Wistar rats after acute exposure – preliminary data
Creators: name: Langa, Ivan
name: Rocha-Pereira, Carolina
name: Milhazes, Nuno
name: Dias Da Silva, Diana
name: Domingues, Susana
name: Silva, Paula
name: Barbosa, Joana
name: Faria, Juliana
name: Tiritan, Maria Elizabeth
name: Ribeiro, Cláudia
Publisher: name: Scientific Letters
Year: 2024

description: Background: There has been a surge in global attention to New Psychoactive Substances (NPS) [1]. Synthetic cathinones stand out as a widely consumed NPS class. Notably, 3-chloromethcathinone (3-CMC) accounted for over 34% of NPS seizures in 2021 [2], which underscores concerns regarding its consumption and health effects. Of note, 3-CMC is chiral and mostly sold as a racemate. As human metabolism and pharmacological effects can be enantioselective [3], determination of the impact of enantioselectivity in toxicokinetics/toxicodynamics is essential for the assessment of 3-CMC effects. Objective: This work aimed to evaluate in vivo the enantioselective biodistribution and toxicity of racemic 3-CMC, after an acute exposure to 3-CMC. Methods: Ten-week-old male Wistar rats were administered intraperitoneally with saline or 3-CMC (10 or 20 mg/kg; n=6). Twenty-four hours after, animals were deeply anesthetized and nine organs (brain, liver, kidneys, lungs, heart, spleen, gut, muscle, adipose tissue), blood and urine were collected. For evaluation of the enantiomeric biodistribution, a previous in house established indirect method by gas chromatography [3], was adapted and validated. Some biochemical analysis was performed using an analyser, whereas TBARS, ATP, glutathione and total protein were determined by spectrophotometry. Organs were also processed for histological analysis. Results: After 24 h, 3-CMC was not found in most organs. Both enantiomers were detected in urine with one dominant enantiomer, suggesting enantioselectivity in metabolism. The histopathological results showed possible central chromatolysis in the brain (20 mg/kg), liver inflammation, renal lesions, lungs’ haemoptysis, and alveolar haemorrhage, in most 3-CMC-exposed animals. No differences were observed in the heart. Conclusions: Our findings show rapid 3-CMC renal elimination, with enantioselectivity in metabolism. Although biochemical evaluations are ongoing, the results are expected to give further insights on the 3-CMC toxicity and histological abnormalities found in the brain, kidneys, liver and lungs.
description: Scientific Letters, Vol. 1 No. Sup 1 (2024)
Temnostoma meridionale Krivosheina & Mamayev 1962
Creators: name: Kočić, Anja
name: Vujić, Ante
name: Tot, Tamara
name: Milosavljević, Marina Janković
name: Groot, Maarten De
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Syrphidae
Temnostoma
Temnostoma meridionale

description: Temnostoma meridionale Krivosheina & Mamayev, 1962.
Regions: CL, LC.
Reference: de Groot & Govedič (2008).
description: Published as part of Kočić, Anja, Vujić, Ante, Tot, Tamara, Milosavljević, Marina Janković & Groot, Maarten De, 2023, An updated checklist of the hoverflies (Diptera: Syrphidae) of Slovenia, pp. 189-227 in Zootaxa 5297 (2) on page 206, DOI: 10.11646/zootaxa.5297.2.2, http://zenodo.org/record/7993180
Assessing the cytotoxic effects of sunitinib on AC16 cardiac cells and its potential association with autophagy
Creators: name: Vitorino-Oliveira, Cláudia
name: Kahremany, Shirin
name: Nisim, Leon
name: Duarte-Araújo, Margarida
name: Carvalho, Félix
name: Gruzman, Arie
name: Costa, Vera Marisa
Publisher: name: Scientific Letters
Year: 2024

description: Background: Cancer survivorship rate has witnessed a notable increase, leveraged by amazing therapeutic advancements. However, these treatments have limitations and problems. Sunitinib (SUN) is a multikinase inhibitor, used in renal cell carcinoma and gastrointestinal stromal tumors. However, its use is limited due to cardiotoxicity, among other adverse effects. Therefore, it is important to understand the mechanisms associated with SUN-induced cardiotoxicity. Autophagy has been linked to SUN cytotoxicity on several models [1]. Objective: To investigate a possible relationship between autophagy and the cytotoxicity induced by SUN in human differentiated AC16 cardiac cells. Methods: AC16 cells were differentiated with horse serum for 24 hours and then exposed to clinically relevant concentrations of SUN (1-20 μM) for 24 or 48 hours, after which two cytotoxicity assays were performed: the MTT reduction and the Neutral Red uptake assays. Then, two working concentrations were chosen (0.01 and 1 μM) to address the effect of some autophagy modulators such as chloroquine (CQ), 3-methyladenine (3-MA) or rapamycin (RAP) on the observed cytotoxicity. Furthermore, the levels of p62, LC3-I e LC3-II were analyzed by western blotting, as well as cellular morphology of cells by phase contrast microscopy. Statistical analysis was performed using two-way ANOVA followed by Sidak’s post hoc test or one-way ANOVA followed by Tukey’s post hoc test, pending on the conditions tested. Results: SUN caused a time- and concentration-dependent cytotoxicity in AC16 cells. Furthermore, SUN seems to accumulate inside AC16 cells at 24-hour exposure in the higher concentration (10 microM). Furthermore, the incubation with SUN 10 microM increases total p62 levels and increases the LC3-II/LC3-I ratio. The results of modulators are ongoing. Conclusions: Our findings demonstrate that SUN induces substantial cytotoxicity in AC16 cells, accompanied by elevated levels of p62 and an increased ratio of LC3-II/LC3-I, indicative of autophagy activation.
description: Scientific Letters, Vol. 1 No. Sup 1 (2024)
Temnostoma vespiforme
Creators: name: Kočić, Anja
name: Vujić, Ante
name: Tot, Tamara
name: Milosavljević, Marina Janković
name: Groot, Maarten De
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Syrphidae
Temnostoma
Temnostoma vespiforme

description: Temnostoma vespiforme (Linnaeus, 1758).
Regions: UC, GO, CL, CS, LC.
Reference: Coe (1960), de Groot & Govedič (2008).
description: Published as part of Kočić, Anja, Vujić, Ante, Tot, Tamara, Milosavljević, Marina Janković & Groot, Maarten De, 2023, An updated checklist of the hoverflies (Diptera: Syrphidae) of Slovenia, pp. 189-227 in Zootaxa 5297 (2) on page 206, DOI: 10.11646/zootaxa.5297.2.2, http://zenodo.org/record/7993180
Exploring the potential of combined natural photosensitizers, gentamycin, and colistin in antimicrobial photodynamic inactivation of Pseudomonas aeruginosa biofilms
Creators: name: Gonçalves, Ariana S. C.
name: Simões, Manuel
name: Borges, Anabela
Publisher: name: Scientific Letters
Year: 2024

description:  Background: The increasing prevalence of wound infections poses a growing threat to the healthcare system [1]. Compounding this challenge, the presence of biofilms significantly impairs both the healing process and the effectiveness of the treatment of wound infections [2]. Antimicrobial photodynamic inactivation (aPDI) has shown promising results in combating this global problem. However, its potential against Gram-negative bacteria such as Pseudomonas aeruginosa remains a major challenge [3]. Objective: The aim of this study is to investigate the effect of subinhibitory concentrations of colistin (CL) in conjugation with curcumin-gentamycin (Cur-Gen) dual combinations on boosting the aPDI of P. aeruginosa biofilms. Methods: The efficacy of CL in enhancing the photodynamic activity of Cur-Gen against biofilms was investigated using the strain P. aeruginosa ATCC 10145. Membrane permeability after CL treatment was assessed by flow cytometry. The synergistic concentrations of the triple combination Cur-Gen-CL were determined using the checkerboard assay. The efficacy of blue light (420 nm, 30 mW/cm2, 10 min) to photoactivate Cur-Gen-CL and thus promote its ability to prevent and control biofilms was investigated. The total mass, metabolic activity and cell culturability of the P. aeruginosa biofilms were quantified by crystal violet, alamar blue and colony forming units (CFU), respectively. Results: Subinhibitory CL concentrations (4 µg/mL) increased the membrane permeability of P. aeruginosa by approximately 30%. This effect enhanced the efficacy of the photoactivated Cur-Gen-CL triple combination in preventing the formation of P. aeruginosa biofilms compared to Cur-Gen alone. In addition, the photoactivated Cur-Gen-CL combination achieved a complete reduction in the culturability (approximately 7 log CFU/cm2) of preformed P. aeruginosa biofilms and a reduction in biomass and metabolic activity by over 60 and 90%, respectively. Conclusions: This study has shown that the use of CL is a promising strategy to enhance the aPDI effect of the Cur-Gen combination against P. aeruginosa biofilms.
description: Scientific Letters, Vol. 1 No. Sup 1 (2024)
Volucella bombylans
Creators: name: Kočić, Anja
name: Vujić, Ante
name: Tot, Tamara
name: Milosavljević, Marina Janković
name: Groot, Maarten De
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Syrphidae
Volucella
Volucella bombylans

description: Volucella bombylans (Linnaeus, 1758).
Regions: UC, GO, CA, CK, CL, LC, SA.
Reference: Scopoli (1763) (as Conops bombilans and Conops pennatus), Strobl (1910), Lambeck (1968), de Groot (2004), de Groot & Govedič (2008), Van Steenis et al. (2013).
description: Published as part of Kočić, Anja, Vujić, Ante, Tot, Tamara, Milosavljević, Marina Janković & Groot, Maarten De, 2023, An updated checklist of the hoverflies (Diptera: Syrphidae) of Slovenia, pp. 189-227 in Zootaxa 5297 (2) on page 207, DOI: 10.11646/zootaxa.5297.2.2, http://zenodo.org/record/7993180
Bacteriocin dynamics in Enterococcus faecium and Enterococcus lactis: implications for clinical and commensal strain interactions
Creators: name: Almeida-Santos, Ana C.
name: Tedim, Ana P.
name: Duarte, Bárbara
name: Brilhante, Michael
name: Coque, Teresa M.
name: Novais, Carla
name: Freitas, Ana R.
name: Peixe, Luísa
Publisher: name: Scientific Letters
Year: 2024

description: Background: Enterococcus faecium-Efm and E.lactis-Elts (former Efm-cladeB) colonize the human gut, with Efm also being a leading hospital-pathogen. Dynamics influencing strain dominance in competitive environments (e.g., infection/colonization) are not fully understood, but bacteriocins may provide competitive advantage to clinical Efm or commensal Elts strains. Objective: We explored bacteriocin content of contemporary Efm and Elts, isolated from healthy/diseased humans, and correlated it with their inhibition profiles against strains across these species. Methods: A collection of 129 strains [91 clinical-Efm (77 vancomycin-resistant-VRE); 35 healthy-volunteers (21-Efm;14-Elts)] from 1996-2022 were challenged against each other by a qualitative bacteriocin production/sensitivity-assay (soft-agar-overlay-technique). Eighty-eight representatives were sequenced (Illumina-NovaSeq) to establish clonality, antibiotic profiles (CGE-tool), and bacteriocins (homemade-database) [1]. Results: Elts (93%) and Efm (87%) carried ≥ 1 bacteriocin. Twenty-one bacteriocins were found, including 8 newly identified. Efm exhibited greater diversity (1-9; x̄=3.6 vs 1-5; x̄=2.6) and both species presented exclusive bacteriocin genes (Efm:bac43/AS5/AS9/enxA/B/entB; Elts:entL50A/B/GM-1). Bacteriocins 43/AS5/AS11/AS9/entA were significantly associated with clinical-Efm-strains (p<0.05), whereas AS8/bac32/entQ/AS4/entl50A/B/GM-1 were exclusive to commensal ones. All were susceptible to inhibition, while 53% of Elts and 65% of Efm (clinical-50%; commensal-39%) inhibited ≥ 1 strain. Those unable to inhibit others were mostly recovered < 2007 or lacked bac43. More bacteriocin genes correlated with less inhibition, and similar profiles resulted in comparable inhibition patterns. Among clinical isolates, ST117, ST78 and ST80 showed a higher inhibitory spectrum. ST78-related strains, particularly ST117, demonstrated activity against ST18-related strains previously dominant in Portuguese hospitals, but not vice-versa. VRE were inhibited by 26% of commensal-strains (Efm/Elts with diverse profiles/STs), while inhibiting up to 85% of them. Conclusions: Distinct bacteriocin profiles in clinical/commensal isolates, coupled with strain-specific and/or mutual strain inhibition dynamics, suggest a competitive landscape for Efm. Commensal strains inhibited VRE, showcasing their potential to counteract resistant strains. This delicate balance, influenced by unknown factors, underscores the valuable insights bacteriocins could provide for future eco-evo strategies combating human infections caused by Efm.
description: Scientific Letters, Vol. 1 No. Sup 1 (2024)
Giorgio Agamben and the Abandonment Paradigm: A New form of Student Diversion in Public Higher Education
Creators: name: Harbour, Clifford P.
name: Wolgemuth, Jennifer R.
Publisher: name: University of Wyoming. Libraries
Year: 2013
Subjects: Education

description: In this article, we propose a new paradigm for understanding recent state policies that pose new barriers to student participation, diverting the most vulnerable students out of public higher education. The paradigm we propose is based on works by the Italian philosopher Giorgio Agamben and is significantly different from previous diversion theories developed by sociologists Burton Clark (1960) and Steven Brint and Jerome Karabel (1989). These earlier theories described how institutional practices and policies diverted students from one curriculum or institution into another. For example, Clark's (1960) case study at one junior college explained how faculty and staff used various academic and student development practices to "cool-out" students and divert them from transfer curricula into vocational curricula. Brint and Karabel's (1989) research examined a rich historical record, and they explained how community colleges secured their future by aggressively marketing vocational curricula that diverted students away from four-year colleges and universities.
New capitalism, risk, and subjectification in an early childhood classroom
Creators: name: Bialostok, Steve (Steven)
name: Kamberelis, George
Publisher: name: University of Wyoming. Libraries
Year: 2010
Subjects: Education

description: New capitalism' has been characterized as an economic period in which insecurity, flux, and uncertainty exist in the workplace. Capitalism attempts to tame that uncertainty through risk taking. Taking risks has become what one must do with risk. Economic discourses of embracing risk-thoroughly grounded in the ideologies of neoliberalism-are widely distributed into many noneconomic areas, including education. Risk taking is now understood as something everyone should valorize, a necessity for freedom and choice. Such discourses are appropriated and recontextualized into different domains, including the everyday interactions between young children and teachers. In this study the authors examine the discourse of a writing lesson taught by one early childhood teacher, focusing on how she used language to promote/produce her students as risk-takers. The authors argue that this teacher's attempt to promote/produce risk-takers belied her commitment to neoliberal ideologies and new modes of capitalism. They also argue that her efforts were dampened by tensions that she also seemed to embody/live-tensions between old and new forms of capitalism, between modernist and postmodernist notions of agency, between safety and risk, and between freedom and control. The outcomes of her work thus seemed to embody tensions in how her students came to understand choice, risk, and freedom.
Volucella inanis
Creators: name: Kočić, Anja
name: Vujić, Ante
name: Tot, Tamara
name: Milosavljević, Marina Janković
name: Groot, Maarten De
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Syrphidae
Volucella
Volucella inanis

description: Volucella inanis (Linnaeus, 1758).
Regions: UC, GO, SS, CL, DR, LC, SA.
Reference: Scopoli (1763) (as Conops trifasciatus) Strobl (1893b, 1894, 1910), Coe (1960), Lambeck (1968), de Groot (2004), de Groot & Govedič (2008).
description: Published as part of Kočić, Anja, Vujić, Ante, Tot, Tamara, Milosavljević, Marina Janković & Groot, Maarten De, 2023, An updated checklist of the hoverflies (Diptera: Syrphidae) of Slovenia, pp. 189-227 in Zootaxa 5297 (2) on page 207, DOI: 10.11646/zootaxa.5297.2.2, http://zenodo.org/record/7993180
Urinary volatile metabolomic biomarkers for detection of bladder cancer
Creators: name: Carapito, Ângela
name: Teixeira-Marques, Ana
name: Jerónimo, Carmen
name: Henrique, Rui
name: A. Roque, Ana Cecília
name: Carvalho, Félix
name: Pinto, Joana
name: Guedes De Pinho, Paula
Publisher: name: Scientific Letters
Year: 2024

description: Background: Early detection of bladder cancer (BC) is necessary for improved outcomes, but current diagnostic methods may lack the necessary sensitivity. Metabolomics, particularly the analysis of urinary volatile organic compounds (VOCs), offers a promising, non-invasive approach to BC detection [1,2]. Objective: The study aimed to investigate potential urinary biomarkers for the detection and staging of BC using metabolomics approaches, in particular gas chromatography-mass spectrometry (GC-MS), and to apply machine learning algorithms to establish a biomarker panel for the accurate detection and staging of BC. Methods: Our study collected urine samples from 196 participants, including 98 BC patients (67 non-muscle invasive bladder cancer, and 31 muscle-invasive bladder cancer) and 98 cancer-free controls. Ethical approval was obtained, and informed consent was signed by all participants. Headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS) was used for volatile profiling in urine. Statistical analyses included principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA), receiver operating characteristic (ROC) analysis, and univariate tests to identify biomarkers for BC detection and staging. Results: Our study found 19 urinary volatile metabolites that effectively discriminate BC patients from cancer-free controls. A panel of ten potential biomarkers was identified for the detection of BC with a sensitivity of 81%, specificity of 90% and accuracy of 85%. This biomarker panel consisted of two aromatic compounds, three ketones, three alcohols, one pyran-like compound, and one fatty acid. Compared to controls, the panel achieved 83% sensitivity, 87% specificity, and 84% accuracy for NMIBC, and 90% sensitivity, 77% specificity, and 87% accuracy for MIBC. No significant differences in urinary volatile profiles were observed between NMIBC and MIBC patients. Conclusions: The identified panel of 10 biomarkers showed significant potential to discriminate between BC patients and controls. While further research is warranted to differentiate NMIBC from MIBC cases, our findings highlight the value of urinary volatile biomarkers in advancing diagnostic approaches for improved patient care.
description: Scientific Letters, Vol. 1 No. Sup 1 (2024)
title: Fetus-Membranen eines plazentalen Säugetieres; [ca. 1930]
title: [Lehrtafel]
titleType: Subtitle
Creators: name: Eichhorn, M.
Publisher: name: Universitätsbibliothek der Ludwig-Maximilians-Universität München
Year: 2023

description: Auf Papier mit Holzbestäbung
Edge-intelligence enabled infield sensing system for heat stress mitigation in apple orchards
Creators: name: Amogi, Basavaraj Rajkumar
Publisher: name: Washington State University
Year: 2023
Subjects: Apple
Edge-computing
Heat wave
Sensing
Agriculture
Weather

description: Heat and water stress can adversely affect apple (Malus domestica Borkh.) tree physiology and cause several fruit disorders (e.g., sunburn). Twining water and heat stress management in summer months is a key challenge to producers in the Pacific Northwest and globally. This is due to lack of reliable stress monitoring technologies and their tie up with management techniques. This dissertation addresses above challenge in-part through development of an improved crop physiology sensing system (CPSS) for heat and water stressors monitoring. The CPSS captures and computes localized weather and thermal-RGB imagery data. In objective 1, a cultivar-, color-, and size-independent mask region based convolutional neural network model was developed for edge deployment on CPSS to achieve season-long fruit surface temperature (FST) monitoring. Algorithm was effective in segmenting fruits (dice coefficient = 0.89) and estimating FST (error <0.5 ℃). FST was estimated within 37s of image capture, about 22%-time improvement over previous method. In objective 2, algorithm was further amended to estimate crop water stress index (CWSI) using hottest and coolest 2% pixels of shaded canopy temperature. Estimated CWSI effectively captured tree water stress and had negative correlation with corresponding stem water potential (r = -0.80). Algorithm’s robustness was evaluated in four heat stress mitigation techniques in commercial ‘Honeycrisp’ production, viz., cyclic conventional evaporative cooling (CEC), fogging, netting, and fognet¬—fogging and netting combined. Treatments had varying effects on CWSI estimates. Developed method found to be more reliable under fogging (r = -0.76) and less reliable under netting (r = -0.65) treatment.
description: Thereafter, objective 3 evaluated efficacy of all four heat stress mitigation techniques for two seasons (2021–2022) using CPSS. Localized weather and FST data indicated that CEC, fogging, and fognet techniques were effective in limiting apple FST below threshold (45 ℃). Fixed cycle frequency of CEC was unreliable in extreme stress events. Fogging had relatively higher, not significant different, sunburn percentage at harvest compared to other treatments. Post-harvest, soft scald and bitter pit incidence were highest, in CEC and fognet, respectively, compared to others. Overall, developed understanding using localized sensing data can increase operational efficacy of mitigation techniques.