title: Ð¤Ð¾Ð»Ð´Ð¸Ð½Ð³ D-Ð³Ð»ÑÐºÐ¾Ð·Ð°/D-Ð³Ð°Ð»Ð°ÐºÑÐ¾Ð·Ð°-ÑÐ²ÑÐ·ÑÐ²Ð°ÑÑÐµÐ³Ð¾ Ð±ÐµÐ»ÐºÐ° Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾Ð³Ð¾ ÐºÑÐ°ÑÐ´Ð¸Ð½Ð³Ð°
title: Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÑÐºÐ°Ñ ÑÐ°Ð±Ð¾ÑÐ°
titleType: Subtitle
Creators: name: Ð¡Ð¸ÑÐ´Ð¸ÐºÐ¾Ð²Ð°, ÐÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2016
Subjects: Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½ÑÐ¹ ÐºÑÐ°ÑÐ´Ð¸Ð½Ð³
ÐºÑÐ°ÑÐ´Ð¸Ð½Ð³-Ð°Ð³ÐµÐ½Ñ
D-Ð³Ð»ÑÐºÐ¾Ð·Ð°/D-Ð³Ð°Ð»Ð°ÐºÑÐ¾Ð·Ð°-ÑÐ²ÑÐ·ÑÐ²Ð°ÑÑÐ¸Ð¹ Ð±ÐµÐ»Ð¾Ðº
Ð¿Ð¾Ð»Ð¸ÑÑÐ¸Ð»ÐµÐ½Ð³Ð»Ð¸ÐºÐ¾Ð»Ñ
Ð´ÐµÐºÑÑÑÐ°Ð½ 70
macromolecular crowding
crowding agent
D-glucose/D-galactose-binding protein
polyethylene-glycol
dextran 70

description: Ð¦ÐµÐ»ÑÑ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÐ»Ð¾ÑÑ Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ ÑÐ²Ð¾ÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ñ/ÑÐ°Ð·Ð²Ð¾ÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ñ D-Ð³Ð»ÑÐºÐ¾Ð·Ð°/D-Ð³Ð°Ð»Ð°ÐºÑÐ¾Ð·Ð°-ÑÐ²ÑÐ·ÑÐ²Ð°ÑÑÐµÐ³Ð¾ Ð±ÐµÐ»ÐºÐ° (GGBP) Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾Ð³Ð¾ ÐºÑÐ°ÑÐ´Ð¸Ð½Ð³Ð°. ÐÐ»Ñ ÑÐ¾Ð·Ð´Ð°Ð½Ð¸Ñ ÑÑÐ»Ð¾Ð²Ð¸Ð¹ ÐºÑÐ°ÑÐ´Ð¸Ð½Ð³Ð° Ð² Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼ÑÑ ÑÐ°ÑÑÐ²Ð¾ÑÐ°Ñ Ð±ÑÐ»Ð¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ ÐºÑÐ°ÑÐ´Ð¸Ð½Ð³-Ð°Ð³ÐµÐ½ÑÑ Ð¿Ð¾Ð»Ð¸ÑÑÐ¸Ð»ÐµÐ½Ð³Ð»Ð¸ÐºÐ¾Ð»Ñ (ÐÐ­Ð) Ð¸ Ð´ÐµÐºÑÑÑÐ°Ð½ 70. ÐÐ¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ ÑÐ´Ð²Ð¸Ð³ ÑÐµÑÐµÐ´Ð¸Ð½Ñ Ð´ÐµÐ½Ð°ÑÑÑÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð³Ð¾ Ð¿ÐµÑÐµÑÐ¾Ð´Ð° GGBP Ð² ÑÑÐ¾ÑÐ¾Ð½Ñ Ð±Ð¾Ð»ÐµÐµ Ð²ÑÑÐ¾ÐºÐ¸Ñ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¹ GdnHCl Ð¿Ð¾ Ð¼ÐµÑÐµ ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ñ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ ÐºÑÐ°ÑÐ´Ð¸Ð½Ð³-Ð°Ð³ÐµÐ½ÑÐ¾Ð² Ð² Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼ÑÑ ÑÐ°ÑÑÐ²Ð¾ÑÐ°Ñ ÑÐ²Ð»ÑÐµÑÑÑ ÑÐ»ÐµÐ´ÑÑÐ²Ð¸ÐµÐ¼ ÑÐ¼ÐµÐ½ÑÑÐµÐ½Ð¸Ñ ÑÐ²Ð¾Ð±Ð¾Ð´Ð½Ð¾Ð³Ð¾ Ð¾Ð±ÑÐµÐ¼Ð° Ð² ÑÐ°ÑÑÐ¼Ð°ÑÑÐ¸Ð²Ð°ÐµÐ¼Ð¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ðµ. Ð£ÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¾, ÑÑÐ¾ ÑÐ²Ð¾ÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ðµ Ð±ÐµÐ»ÐºÐ° Ð² Ð²ÑÑÐ¾ÐºÐ¾ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ ÑÐ°ÑÑÐ²Ð¾ÑÐ°Ñ ÐÐ­Ð Ð¸ Ð´ÐµÐºÑÑÑÐ°Ð½Ð° ÑÐ¾Ð¿ÑÐ¾Ð²Ð¾Ð¶Ð´Ð°ÐµÑÑÑ Ð°Ð³ÑÐµÐ³Ð°ÑÐ¸ÐµÐ¹ GGBP. ÐÑÐ¸ ÑÑÐ¾Ð¼ ÐÐ­Ð ÑÐ¿Ð¾ÑÐ¾Ð±ÐµÐ½ Ð¼ÐµÐ´Ð»ÐµÐ½Ð½Ð¾ Ð´ÐµÑÑÐ°Ð±Ð¸Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°ÑÑ Ð±ÐµÐ»Ð¾Ðº Ð² Ð½ÐµÐ·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾ÑÑÐ¸ Ð¾Ñ Ð¿ÑÐ¸ÑÑÑÑÑÐ²Ð¸Ñ Ð² ÑÐ°ÑÑÐ²Ð¾ÑÐµ Ð´ÐµÐ½Ð°ÑÑÑÐ°Ð½ÑÐ°. ÐÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼Ð¾ Ð¾ÑÐ¼ÐµÑÐ¸ÑÑ, ÑÑÐ¾ Ð¿ÑÐ¸ Ð²ÑÑÐ¾ÐºÐ¸Ñ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸ÑÑ Ð´ÐµÐºÑÑÑÐ°Ð½Ð° ÑÑÐ¾Ñ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑ, Ð¿Ð¾-Ð²Ð¸Ð´Ð¸Ð¼Ð¾Ð¼Ñ, Ð¼Ð¾Ð¶ÐµÑ ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐµÑÐºÐ¸ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¾Ð²Ð°ÑÑ Ñ GGBP, Ð¸Ð·Ð¼ÐµÐ½ÑÑ Ð¿ÑÐ¸ ÑÑÐ¾Ð¼ ÐºÐ¾Ð½ÑÐ¾ÑÐ¼Ð°ÑÐ¸Ñ Ð±ÐµÐ»ÐºÐ°.
description: The aim of this work was to investigate the folding of D-glucose/D-galactose-binding protein (GGBP) in the macromolecular crowding. To simulate the crowding conditions were used crowding agents polyethylene-glycol (PEG) and dextran 70. Shown, that the shift of the middle GGBP denaturation transition towards higher GdnHCl concentrations with increasing concentrations of crowding agents in the solutions is the result of reducing the free volume in the system. It was found, that the protein folding in highly concentrated solutions of dextran and PEG followed by aggregation GGBP. In addition PEG is able to destabilize the protein slowly regardless of the presence of the denaturant in the solution. It should be noted that at high concentrations the dextran, polymer apparently can interact specifically with GGBP, altering the conformation of the protein.