Colwellia mytili Kim & Park & Park & Nam & Jung & Kim & Yoon 2017, SP. NOV.
Creators: name: Kim, Young-Ok
name: Park, In-Suk
name: Park, Sooyeon
name: Nam, Bo-Hye
name: Jung, Yong-Taek
name: Kim, Dong-Gyun
name: Yoon, Jung-Hoon
Publisher: name: Zenodo
Year: 2017
Subjects: Biodiversity
Taxonomy
Bacteria
Proteobacteria
Gammaproteobacteria
Alteromonadales
Colwelliaceae
Colwellia
Colwellia mytili

description: DESCRIPTION OF COLWELLIA MYTILI SP. NOV.   
 Colwellia mytili (my′ ti.li. L. gen. n. mytili of Mytilus, named after the generic name of the mussel Mytilus edulis, from which the type strain was isolated).   
 Cells are Gram-stain-negative, non-spore-forming, rodshaped, approximately 0.3–0.7 µm in diameter and 0.5–8.0 µm in length, and motile by means of a single polar flagellum. Colonies on MA are circular, slightly convex, glistening, smooth, greyish-yellow and 0.8–1.5 mm after incubation for 5 days at 20 Ǫ C. Optimal growth is observed at 20 Ǫ C; growth occurs at 4 Ǫ C and slowly at 25 Ǫ C, but not at 28 Ǫ C. Optimal pH for growth is between 7.0 and 8.0; growth occurs at pH 6.0, but not at pH 5.5. Growth occurs with 1.5–4.0 % (w/v) NaCl (optimum 2.0–3.0 %). Growth does not occur under anaerobic conditions on MA and on MA supplemented with nitrate. Catalase- and oxidase-positive. Nitrate is not reduced to nitrite. Casein, starch and Tween 80 are hydrolysed, but aesculin, gelatin, hypoxanthine, L- tyrosine, urea and xanthine are not. Pyruvate is utilized as carbon and energy source, but L- arabinose, cellobiose, D- fructose, D- galactose, D- glucose, maltose, D- mannose, sucrose, trehalose, D- xylose, acetate, benzoate, citrate, formate, L- malate, succinate, salicin and L-glutamate are not. In assays with the API ZYM system, activity of alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase and acid phosphatase is present and activity of naphthol-AS-BI-phosphohydrolase is weakly present, but activity of lipase (C14), valine arylamidase, cystine arylamidase, trypsin, a- chymotrypsin, a- galactosidase, b-galactosidase, b- glucuronidase, a- glucosidase, b- glucosidase, N -acetyl-b- glucosaminidase, a- mannosidase and a- fucosidase is absent. Susceptible to ampicillin, carbenicillin, cephalotin, chloramphenicol, gentamicin, novobiocin, oleandomycin, penicillin G, polymyxin B and streptomycin, but not to kanamycin, lincomycin, neomycin and tetracycline. The predominant ubiquinone is Q-8. The major fatty acids (>10 % of the total fatty acids) are summed feature 3 (C 16: 1 Ɯ7c and/or C 16: 1 Ɯ6c) and C 16: 0. The major polar lipids are phosphatidylglycerol and phosphatidylethanolamine.   
 *Summed feature is group of two or more fatty acids that could not be separated using MIDI system. Summed feature 3 contains C16: 1 Ɯ7c and/or C16: 1 Ɯ6c.   
 The type strain, RA2-7 T (= KCTC 52417 T = NBRC 112381 T), was isolated a mussel (Mytilus edulis) collected from Cheongsan island in the South Sea, South Korea. The DNA G+C content of the type strain is 39.0±0.04 mol %.
description: Published as part of Kim, Young-Ok, Park, In-Suk, Park, Sooyeon, Nam, Bo-Hye, Jung, Yong-Taek, Kim, Dong-Gyun & Yoon, Jung-Hoon, 2017, Colwellia mytili sp. nov., isolated from mussel Mytilus edulis, pp. 31-36 in International Journal of Systematic and Evolutionary Microbiology 67 (1) on pages 34-35, DOI: 10.1099/ijsem.0.001564, http://zenodo.org/record/6048369