title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð² EDR Ð¸ KED Ð½Ð° Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ñ Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÑ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð² Ð½ÐµÐ¹ÑÐ¾Ð½Ð¾Ð² Ð¸ Ð½ÐµÐ¹ÑÐ¾Ð¿Ð»Ð°ÑÑÐ¸ÑÐ½Ð¾ÑÑÑ Ð² Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ ÐÐ»ÑÑÐ³ÐµÐ¹Ð¼ÐµÑÐ° in vivo
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: EDR and KED peptides affect on the dendritic neuronal spines morphology and neuroplasticity in the Alzheimer's disease model in vivo
Creators: name: ÐÐ»ÑÐ¸Ð½Ð°, ÐÐ½Ð°ÑÑÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: Ð±Ð¾Ð»ÐµÐ·Ð½Ñ ÐÐ»ÑÑÐ³ÐµÐ¹Ð¼ÐµÑÐ°
Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÐµ ÑÐ¸Ð¿Ð¸ÐºÐ¸
Ð´Ð¾Ð»Ð³Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð°Ñ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°ÑÐ¸Ñ
Ð¿ÐµÐ¿ÑÐ¸Ð´Ð½ÑÐµ Ð±Ð¸Ð¾ÑÐµÐ³ÑÐ»ÑÑÐ¾ÑÑ
Alzheimer's disease
dendritic spines
long-term potentiation
peptides

description: ÐÐ»Ñ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð¿Ð°ÑÐ¾Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹ Ð½ÐµÐ¹ÑÐ¾Ð½Ð¾Ð² Ð¡Ð1 Ð¾Ð±Ð»Ð°ÑÑÐ¸ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð° Ð¿ÑÐ¸ ÐÐ in vivo Ð±ÑÐ»Ð° Ð¿Ð¾Ð»ÑÑÐµÐ½Ð° Ð½Ð¾Ð²Ð°Ñ ÑÑÐ°Ð½ÑÐ³ÐµÐ½Ð½Ð°Ñ Ð»Ð¸Ð½Ð¸Ñ Ð¼ÑÑÐµÐ¹ 5ÑFAD-M. ÐÐ½ÑÑÑÐ¸Ð±ÑÑÑÐ¸Ð½Ð½Ð¾Ðµ Ð²Ð²ÐµÐ´ÐµÐ½Ð¸Ðµ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð² EDR Ð¸ KED Ð¼ÑÑÐ°Ð¼ Ð»Ð¸Ð½Ð¸Ð¸ 5xFAD ÑÐ¿Ð¾ÑÐ¾Ð±ÑÑÐ²Ð¾Ð²Ð°Ð»Ð¾ ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ñ ÐÐÐ Ñ ÑÑÐ¸Ñ Ð¼ÑÑÐµÐ¹ ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²ÐµÐ½Ð½Ð¾ Ð¿Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ Ñ ÑÑÐ¸Ð¼ Ð¿Ð¾ÐºÐ°Ð·Ð°ÑÐµÐ»ÐµÐ¼ Ñ Ð¼ÑÑÐµÐ¹ Ð»Ð¸Ð½Ð¸Ð¸ 5xFAD, Ð¿Ð¾Ð»ÑÑÐ°Ð²ÑÐ¸Ñ ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¹ ÑÐ°ÑÑÐ²Ð¾Ñ. ÐÐµÐ¿ÑÐ¸Ð´ KED Ð½Ð¾ÑÐ¼Ð°Ð»Ð¸Ð·Ð¾Ð²ÑÐ²Ð°Ð» Ð½ÐµÐ¹ÑÐ¾Ð¿Ð»Ð°ÑÑÐ¸ÑÐ½Ð¾ÑÑÑ Ð² Ð¡Ð1 Ð¾Ð±Ð»Ð°ÑÑÐ¸ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð° Ð¼ÑÑÐµÐ¹ Ð»Ð¸Ð½Ð¸Ð¸ 5ÑFAD Ð´Ð¾ ÑÑÐ¾Ð²Ð½Ñ Ð¼ÑÑÐµÐ¹ Ð´Ð¸ÐºÐ¾Ð³Ð¾ ÑÐ¸Ð¿Ð°. ÐÐ½ÑÑÑÐ¸Ð±ÑÑÑÐ¸Ð½Ð½Ð¾Ðµ Ð²Ð²ÐµÐ´ÐµÐ½Ð¸Ðµ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð² EDR Ð¸ KED Ð² ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ 400 Ð¼ÐºÐ³/ÐºÐ³ Ð¼ÑÑÐ°Ð¼ Ð»Ð¸Ð½Ð¸Ð¸ 5xFAD-M Ð¿ÑÐ¸Ð²Ð¾Ð´Ð¸Ð»Ð¾ Ðº ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ñ Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÐ¸ Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÑ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð² Ð½ÐµÐ¹ÑÐ¾Ð½Ð¾Ð² Ð¡Ð1 5xFAD-Ð, ÐºÐ¾ÑÐ¾ÑÑÐ¼ Ð²Ð²Ð¾Ð´Ð¸Ð»Ð¸ ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ°ÑÑÐ²Ð¾Ñ. ÐÐµÐ¿ÑÐ¸Ð´ KED ÑÐ¿Ð¾ÑÐ¾Ð±ÑÑÐ²Ð¾Ð²Ð°Ð» ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ñ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð° Ð³ÑÐ¸Ð±Ð¾Ð²Ð¸Ð´Ð½ÑÑ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð² Ð½Ð° 35% Ð¿Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ Ñ ÑÑÐ¸Ð¼ Ð¿Ð¾ÐºÐ°Ð·Ð°ÑÐµÐ»ÐµÐ¼ Ñ Ð¼ÑÑÐµÐ¹ Ð»Ð¸Ð½Ð¸Ð¸ 5ÑFAD-Ð, ÐºÐ¾ÑÐ¾ÑÑÐ¼ Ð²Ð²Ð¾Ð´Ð¸Ð»Ð¸ ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ°ÑÑÐ²Ð¾Ñ. ÐÐ»Ñ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð¸Ñ Ð¿Ð¾ÑÑÐ°Ð²Ð»ÐµÐ½Ð½ÑÑ Ð·Ð°Ð´Ð°Ñ Ð±ÑÐ»Ð¸ Ð¾ÑÐ²Ð¾ÐµÐ½Ñ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð¼ÐµÑÐ¾Ð´Ñ: Ð³ÐµÐ½Ð¾ÑÐ¸Ð¿Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð¼ÑÑÐµÐ¹, Ð¿ÑÐ¸Ð³Ð¾ÑÐ¾Ð²Ð»ÐµÐ½Ð¸Ðµ ÑÐ¸ÐºÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ Ð¸ Ð¿ÐµÑÐµÐ¶Ð¸Ð²Ð°ÑÑÐ¸Ñ ÑÑÐµÐ·Ð¾Ð² Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð°, ÑÑÐ°Ð½ÑÐºÐ°ÑÐ´Ð¸Ð°Ð»ÑÐ½Ð°Ñ Ð¿ÐµÑÑÑÐ·Ð¸Ñ, Ð°Ð½Ð°Ð»Ð¸Ð· Ð¼Ð¸ÐºÑÐ¾ÑÐ¾ÑÐ¾Ð³ÑÐ°ÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð¸Ð·Ð¾Ð±ÑÐ°Ð¶ÐµÐ½Ð¸Ð¹, Ð¸Ð·Ð¼ÐµÑÐµÐ½Ð¸Ðµ Ð¿Ð¾Ð»ÐµÐ²ÑÑ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð¾Ð² Ð²Ð½ÐµÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð½ÐµÐ¹ÑÐ¾Ð½Ð¾Ð² Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð°.
description: New transgenic 5xFAD-M mice line was obtained in order to estimate pathomorphological changes of neurons in CA1 region of the hippocampus. Intraperitoneal injections of EDR and KED peptides to 5xFAD mice contributed to an increase in LTP in these mice compared to 5xFAD, which were injected with saline solution. The KED peptide normalized neuroplasticity in the CA1 region of the hippocampus of 5xFAD mice up to the level of wild-type mice. Intraperitoneal injections of EDR and KED peptides to 5xFAD-M mice resulted in an increase in the dendritic spines density of neurons from CA1 region of the hippocampus compared to saline injections. The KED peptide increases the number of mushroom spines on 35% compared to 5xFAD-M mice that were injected with saline. To perform the tasks, the following methods were mastered: mouse genotyping, preparation of fixed and surviving sections of the hippocampus, transcardial perfusion, analysis of microphotographic images, measurement of field potentials of extracellular activity of hippocampal neurons.