title: Electrophoretic laser light scattering technique for zeta-potentional determination in fluids
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: ÐÐµÑÐ¾Ð´ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð»Ð°Ð·ÐµÑÐ½Ð¾Ð³Ð¾ ÑÐ°ÑÑÐµÑÐ½Ð¸Ñ ÑÐ²ÐµÑÐ° Ð´Ð»Ñ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð´Ð·ÐµÑÐ°-Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð° ÑÐ°ÑÑÐ¸Ñ Ð² ÑÐ°ÑÑÐ²Ð¾ÑÐ°Ñ
Creators: name: ÐÑÐ½ Ð¥Ð°Ð½ÑÑÑÐ½
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð¡Ð²ÐµÑ
electrophoretic light scattering
nanoparticles
zeta potential
fluids

description: The focus of the graduate research project is on the utilization of the electrophoretic laser light scattering technique for determining zeta-potential in biological fluids. The primary subject under investigation is the zeta potential of colloids, a crucial parameter for comprehending the stability and behavior of colloidal dispersions. The study involved a comprehensive literature review on various methods for examining the electrokinetic properties of nanoparticles, including electrophoresis, laser Doppler electrophoresis, electro-osmosis, flow potential, and flow current techniques. Among these methods, electrophoretic light scattering emerged as the most promising for analyzing the electrokinetic properties of nanoparticles, with its applicability being justified. The research delved into the physical and mathematical principles of the electrophoretic light scattering method, along with its experimental implementation. The relationship between electrophoretic mobility and zeta potential was explored, leading to the development and assembly of an experimental setup based on this method. The setup was optimized using latex microspheres of varying sizes, and calculations were performed to determine electrophoretic mobility and zeta potential. A comparison table was created to contrast the values obtained from the laboratory setup with those from a commercial Zetasizer Nano device, revealing a high level of agreement with errors within 20% for most values. Additionally, the text investigates the electrokinetic characteristics of albumin, including its charge and stability in response to external factors such as electrolytes and changes in solution pH. The studys significance lies in enhancing the understanding of zeta potential assessment in biological fluids, which is crucial for applications in medicine, pharmaceuticals, and biotechnology. By enhancing the precision and efficiency of zeta potential determination, this research contributes to the advancement of colloidal science and the development of new technologies.
description: ÐÑÐ½Ð¾Ð²Ð½Ð¾Ðµ Ð²Ð½Ð¸Ð¼Ð°Ð½Ð¸Ðµ Ð² Ð´Ð¸Ð¿Ð»Ð¾Ð¼Ð½Ð¾Ð¼ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÑÐºÐ¾Ð¼ Ð¿ÑÐ¾ÐµÐºÑÐµ ÑÐ´ÐµÐ»ÑÐµÑÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¼ÐµÑÐ¾Ð´Ð° ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð»Ð°Ð·ÐµÑÐ½Ð¾Ð³Ð¾ ÑÐ°ÑÑÐµÑÐ½Ð¸Ñ ÑÐ²ÐµÑÐ° Ð´Ð»Ñ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð´Ð·ÐµÑÐ°-Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð° Ð² Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð¶Ð¸Ð´ÐºÐ¾ÑÑÑÑ. ÐÑÐ½Ð¾Ð²Ð½ÑÐ¼ Ð¿ÑÐµÐ´Ð¼ÐµÑÐ¾Ð¼ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐ²Ð»ÑÐµÑÑÑ Ð´Ð·ÐµÑÐ°-Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð» ÐºÐ¾Ð»Ð»Ð¾Ð¸Ð´Ð¾Ð², Ð²Ð°Ð¶Ð½ÐµÐ¹ÑÐ¸Ð¹ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑ Ð´Ð»Ñ Ð¿Ð¾Ð½Ð¸Ð¼Ð°Ð½Ð¸Ñ ÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾ÑÑÐ¸ Ð¸ Ð¿Ð¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ ÐºÐ¾Ð»Ð»Ð¾Ð¸Ð´Ð½ÑÑ Ð´Ð¸ÑÐ¿ÐµÑÑÐ¸Ð¹. ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð²ÐºÐ»ÑÑÐ°Ð»Ð¾ Ð² ÑÐµÐ±Ñ Ð²ÑÐµÑÑÐ¾ÑÐ¾Ð½Ð½Ð¸Ð¹ Ð¾Ð±Ð·Ð¾Ñ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÑ Ð¿Ð¾ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÐ¼ Ð¼ÐµÑÐ¾Ð´Ð°Ð¼ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐ»ÐµÐºÑÑÐ¾ÐºÐ¸Ð½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÐ²Ð¾Ð¹ÑÑÐ² Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸Ñ, Ð²ÐºÐ»ÑÑÐ°Ñ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÐ·, Ð»Ð°Ð·ÐµÑÐ½ÑÐ¹ Ð´Ð¾Ð¿Ð»ÐµÑÐ¾Ð²ÑÐºÐ¸Ð¹ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÐ·, ÑÐ»ÐµÐºÑÑÐ¾Ð¾ÑÐ¼Ð¾Ñ, Ð¼ÐµÑÐ¾Ð´Ñ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð° Ð¿Ð¾ÑÐ¾ÐºÐ° Ð¸ ÑÐ¾ÐºÐ° Ð¿Ð¾ÑÐ¾ÐºÐ°. Ð¡ÑÐµÐ´Ð¸ ÑÑÐ¸Ñ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÑÐ¸ÑÐµÑÐºÐ¾Ðµ ÑÐ°ÑÑÐµÑÐ½Ð¸Ðµ ÑÐ²ÐµÑÐ° Ð¾ÐºÐ°Ð·Ð°Ð»Ð¾ÑÑ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ Ð¿ÐµÑÑÐ¿ÐµÐºÑÐ¸Ð²Ð½ÑÐ¼ Ð´Ð»Ñ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° ÑÐ»ÐµÐºÑÑÐ¾ÐºÐ¸Ð½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÐ²Ð¾Ð¹ÑÑÐ² Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸Ñ, Ð¸ ÐµÐ³Ð¾ Ð¿ÑÐ¸Ð¼ÐµÐ½Ð¸Ð¼Ð¾ÑÑÑ Ð±ÑÐ»Ð° Ð¾Ð±Ð¾ÑÐ½Ð¾Ð²Ð°Ð½Ð°. ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ ÑÐ³Ð»ÑÐ±Ð»ÑÐ»Ð¾ÑÑ Ð² ÑÐ¸Ð·Ð¸ÑÐµÑÐºÐ¸Ðµ Ð¸ Ð¼Ð°ÑÐµÐ¼Ð°ÑÐ¸ÑÐµÑÐºÐ¸Ðµ Ð¿ÑÐ¸Ð½ÑÐ¸Ð¿Ñ Ð¼ÐµÑÐ¾Ð´Ð° ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐ°ÑÑÐµÑÐ½Ð¸Ñ ÑÐ²ÐµÑÐ°, Ð° ÑÐ°ÐºÐ¶Ðµ ÐµÐ³Ð¾ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ ÑÐµÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ñ. ÐÑÐ»Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð° ÑÐ²ÑÐ·Ñ Ð¼ÐµÐ¶Ð´Ñ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¿Ð¾Ð´Ð²Ð¸Ð¶Ð½Ð¾ÑÑÑÑ Ð¸ Ð´Ð·ÐµÑÐ°-Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð¾Ð¼, ÑÑÐ¾ Ð¿ÑÐ¸Ð²ÐµÐ»Ð¾ Ðº ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐµ Ð¸ ÑÐ±Ð¾ÑÐºÐµ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½Ð¾Ð¹ ÑÑÑÐ°Ð½Ð¾Ð²ÐºÐ¸ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ ÑÑÐ¾Ð³Ð¾ Ð¼ÐµÑÐ¾Ð´Ð°. Ð£ÑÑÐ°Ð½Ð¾Ð²ÐºÐ° Ð±ÑÐ»Ð° Ð¾Ð¿ÑÐ¸Ð¼Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°Ð½Ð° Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð»Ð°ÑÐµÐºÑÐ½ÑÑ Ð¼Ð¸ÐºÑÐ¾ÑÑÐµÑ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ ÑÐ°Ð·Ð¼ÐµÑÐ¾Ð², Ð¸ Ð±ÑÐ»Ð¸ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ñ ÑÐ°ÑÑÐµÑÑ Ð´Ð»Ñ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¿Ð¾Ð´Ð²Ð¸Ð¶Ð½Ð¾ÑÑÐ¸ Ð¸ Ð´Ð·ÐµÑÐ°-Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð°. ÐÑÐ»Ð° ÑÐ¾Ð·Ð´Ð°Ð½Ð° ÑÑÐ°Ð²Ð½Ð¸ÑÐµÐ»ÑÐ½Ð°Ñ ÑÐ°Ð±Ð»Ð¸ÑÐ° Ð´Ð»Ñ ÑÐ¾Ð¿Ð¾ÑÑÐ°Ð²Ð»ÐµÐ½Ð¸Ñ Ð·Ð½Ð°ÑÐµÐ½Ð¸Ð¹, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð½Ð° Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ½Ð¾Ð¹ ÑÑÑÐ°Ð½Ð¾Ð²ÐºÐµ, ÑÐ¾ Ð·Ð½Ð°ÑÐµÐ½Ð¸ÑÐ¼Ð¸, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÐ¼Ð¸ Ð½Ð° ÐºÐ¾Ð¼Ð¼ÐµÑÑÐµÑÐºÐ¾Ð¼ ÑÑÑÑÐ¾Ð¹ÑÑÐ²Ðµ Zetasizer Nano, ÑÑÐ¾ Ð²ÑÑÐ²Ð¸Ð»Ð¾ Ð²ÑÑÐ¾ÐºÐ¸Ð¹ ÑÑÐ¾Ð²ÐµÐ½Ñ ÑÐ¾Ð³Ð»Ð°ÑÐ¸Ñ Ñ Ð¾ÑÐ¸Ð±ÐºÐ°Ð¼Ð¸ Ð² Ð¿ÑÐµÐ´ÐµÐ»Ð°Ñ 20% Ð´Ð»Ñ Ð±Ð¾Ð»ÑÑÐ¸Ð½ÑÑÐ²Ð° Ð·Ð½Ð°ÑÐµÐ½Ð¸Ð¹. ÐÑÐ¾Ð¼Ðµ ÑÐ¾Ð³Ð¾, Ð² ÑÐµÐºÑÑÐµ Ð¸ÑÑÐ»ÐµÐ´ÑÑÑÑÑ ÑÐ»ÐµÐºÑÑÐ¾ÐºÐ¸Ð½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ðµ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ¸ Ð°Ð»ÑÐ±ÑÐ¼Ð¸Ð½Ð°, Ð²ÐºÐ»ÑÑÐ°Ñ ÐµÐ³Ð¾ Ð·Ð°ÑÑÐ´ Ð¸ ÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾ÑÑÑ Ð² Ð¾ÑÐ²ÐµÑ Ð½Ð° Ð²Ð½ÐµÑÐ½Ð¸Ðµ ÑÐ°ÐºÑÐ¾ÑÑ, ÑÐ°ÐºÐ¸Ðµ ÐºÐ°Ðº ÑÐ»ÐµÐºÑÑÐ¾Ð»Ð¸ÑÑ Ð¸ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ pH ÑÐ°ÑÑÐ²Ð¾ÑÐ°. ÐÐ½Ð°ÑÐ¸Ð¼Ð¾ÑÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ Ð·Ð°ÐºÐ»ÑÑÐ°ÐµÑÑÑ Ð² ÑÐ»ÑÑÑÐµÐ½Ð¸Ð¸ Ð¿Ð¾Ð½Ð¸Ð¼Ð°Ð½Ð¸Ñ Ð¾ÑÐµÐ½ÐºÐ¸ Ð´Ð·ÐµÑÐ°-Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð° Ð² Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð¶Ð¸Ð´ÐºÐ¾ÑÑÑÑ, ÑÑÐ¾ Ð¸Ð¼ÐµÐµÑ ÑÐµÑÐ°ÑÑÐµÐµ Ð·Ð½Ð°ÑÐµÐ½Ð¸Ðµ Ð´Ð»Ñ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð² Ð¼ÐµÐ´Ð¸ÑÐ¸Ð½Ðµ, ÑÐ°ÑÐ¼Ð°ÑÐµÐ²ÑÐ¸ÐºÐµ Ð¸ Ð±Ð¸Ð¾ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¸. ÐÐ¾Ð²ÑÑÐ°Ñ ÑÐ¾ÑÐ½Ð¾ÑÑÑ Ð¸ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð´Ð·ÐµÑÐ°-Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð°, ÑÑÐ¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð²Ð½Ð¾ÑÐ¸Ñ Ð²ÐºÐ»Ð°Ð´ Ð² ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ðµ ÐºÐ¾Ð»Ð»Ð¾Ð¸Ð´Ð½Ð¾Ð¹ Ð½Ð°ÑÐºÐ¸ Ð¸ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÑ Ð½Ð¾Ð²ÑÑ ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¹.