Differential expression of genes implicated in liver lipid metabolism in broiler chickens differing in weight
Creators: name: N. Pirany
name: N. PIRANY
name: A. Bakrani Balani
name: A. BAKRANI BALANI
name: H. Hassanpour
name: H. HASSANPOUR
name: H. Mehraban
name: H. MEHRABAN
Publisher: name: Taylor & Francis
Year: 2019
Subjects: subject: Biochemistry
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Physiology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: FOS: Chemical sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: 60506 Virology
subject: Computational Biology

description: Lipid parameters and expression of <i>ACACA, APOA1, CPT1A, FASN, FOXO1, LIPG, PPARα</i> and <i>SIRT1</i> genes involved in lipid metabolism were investigated in two groups of high (HW) and low (LW) weight broilers from the same strain.Blood cholesterol and liver triglyceride levels were significantly increased in HW chickens compared to LW broilers, while other parameters i.e. blood triglyceride, blood HDL/LDL, liver cholesterol and total liver fat showed no significant changes in either group.The relative expression of <i>ACACA, APOA1</i> and <i>CPT1A</i> genes was significantly lower in the liver tissues of HW broilers than in the LW group. The mRNA levels of these three genes showed a significant negative correlation with abdominal fat deposition and live weight of broilers. However, relative expression of <i>FASN, FOXO1, LIPG, PPARα</i> and <i>SIRT1</i> hepatic genes did not differ among broilers.It was concluded that, of eight hepatic genes implicated in lipid metabolism, only the expression of three (<i>ACACA, APOA1</i> and <i>CPT1A</i>) were significant for fat and leanness within the same strain of chicken. Since reducing body fat is a major goal in the broiler industry, these data can provide fresh insight into the molecular processes underlying the regulation of fat deposition in broilers. Lipid parameters and expression of <i>ACACA, APOA1, CPT1A, FASN, FOXO1, LIPG, PPARα</i> and <i>SIRT1</i> genes involved in lipid metabolism were investigated in two groups of high (HW) and low (LW) weight broilers from the same strain. Blood cholesterol and liver triglyceride levels were significantly increased in HW chickens compared to LW broilers, while other parameters i.e. blood triglyceride, blood HDL/LDL, liver cholesterol and total liver fat showed no significant changes in either group. The relative expression of <i>ACACA, APOA1</i> and <i>CPT1A</i> genes was significantly lower in the liver tissues of HW broilers than in the LW group. The mRNA levels of these three genes showed a significant negative correlation with abdominal fat deposition and live weight of broilers. However, relative expression of <i>FASN, FOXO1, LIPG, PPARα</i> and <i>SIRT1</i> hepatic genes did not differ among broilers. It was concluded that, of eight hepatic genes implicated in lipid metabolism, only the expression of three (<i>ACACA, APOA1</i> and <i>CPT1A</i>) were significant for fat and leanness within the same strain of chicken. Since reducing body fat is a major goal in the broiler industry, these data can provide fresh insight into the molecular processes underlying the regulation of fat deposition in broilers.