121. Chemokine profile in the peripheral blood and vascular tissue of patients with Takayasu arteritis
Creators: name: Xiufang Kong1
name: Sifan Wu1
name: Xiaojuan Dai1
name: Wensu Yu1
name: Jinghua Wang1
name: Sun1, Ying
name: Zongfei Ji1
name: Lingying Ma1
name: Xiaomin Dai1
name: Huiyong  Chen1
name: Ma1, Lili
name: Lindi Jiang1, 2
Publisher: name: Zenodo
Year: 2022
Subjects: Vasculitis
ANCA
Abstracts
Giant Cell Arthritis
MPA
GPA
EGPA
Takayasu
IgA vasculitis

description: <strong>Background:</strong> Takayasu arteritis (TAK) is characterized by chronic inflammation, and multiple immune cells participate in the development of TAK. The aim of this study is to explore the chemokine spectrum in peripheral blood as well as vascular tissue from patients with TAK, and investigate its potential role in the pathogenesis of TAK. <strong>Methods: </strong>A total of 58 naive patients with TAK and 53 healthy controls were enrolled in this study from Zhongshan Hospital, Fudan University, Shanghai, China, between January 1st and December 31st, 2020. Among the study participants, 50 patients with TAK and 48 healthy controls were selected for serum examination, while the remaining 8 patients and 5 controls were selected for vascular tissue examination. <strong>Results: </strong> The patients with TAK and healthy controls selected for serum examination were matched for age (TAK: 34.44 ± 13.25 years, Control: 37.85 ± 5.10 years, p = 0.27) and female:male ratio (TAK: 41:9, Control: 36:12, p = 0.40). Among the 50 patients with TAK, 40 (80%) had active disease at the time of enrollment. No significant differences were observed in the clinical characteristics (p &gt; 0.05 for all parameters) of patients (n = 5) selected for chemokine screening and those selected for chemokine validation (n = 45). The main reasons for surgery were aortic regurgitation (n = 7) and renal artery occlusion (n = 1). Thus, ascending aortic specimens (n = 7) and a renal artery specimen (n = 1) were obtained. In addition, apparently normal specimens (control) of the ascending aorta (n = 3) and abdominal aorta (n = 2) were obtained from donors for heart transplantation (n = 3) and liver transplantation (n = 2). Increased expression of CCL22, RANTES, CXCL16, CXCL11, and IL-16 in the peripheral blood of patients with TAK. Among the 31 chemokines, the average signal of 12 chemokines was over 15% higher in patients with TAK than in the healthy controls: CCL22, RANTES, CXCL16, CXCL11, IL-16, CCL1, XCL1, CX3CL1, CXCL1, CCL17, CCL19, and CCL20. In contrast, the average signal of four chemokines (CXCL10, CXCL7, CCL18, and CXCL4) was 15% lower in patients with TAK than in the healthy controls. Correlations between the changes in these chemokines were also analyzed, and the results indicated similar trends in the changes in CCL22, CXCL16, and IL-16 levels after treatment. A proposed model illustrating chemokines discovered in the present study and their potential role in the pathogenesis of TAK was shown in Figure 1. <strong>Conclusions:</strong> CCL22, RANTES, CXCL16, CXCL11, and IL-16 are identified as the major chemokines that may involve in the recruitment of immune cells in the vascular tissue of patients with TAK. Additionally, the persistently high levels of CCL22, CXCL11, and IL-16 observed after treatment might suggest their participation in vascular chronic inflammation or fibrosis and demonstrate the need for developing more efficacious treatment options. <strong>Disclosures:</strong> none <strong>Figure 1.</strong>