cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
An optimised spectrophotometric assay for convenient and accurate quantitation of intracellular iron from iron oxide nanoparticles
Creators: name: Hedayati, Mohammad
name: Abubaker-Sharif, Bedri
name: Khattab, Mohamed
name: Razavi, Allen
name: Mohammed, Isa
name: Arsalan Nejad
name: Wabler, Michele
name: Haoming Zhou
name: Mihalic, Jana
name: Gruettner, Cordula
name: DeWeese, Theodore
name: Ivkov, Robert
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Biochemistry
subject: Space Science
subject: Microbiology
subject: FOS: Biological sciences
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: Inorganic Chemistry
subject: Hematology
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: We report the development and optimisation of an assay for quantitating iron from iron oxide nanoparticles in biological matrices by using ferene-s, a chromogenic compound. The method is accurate, reliable and can be performed with basic equipment common to many laboratories making it convenient and inexpensive. The assay we have developed is suited for quantitation of iron in cell culture studies with iron oxide nanoparticles, which tend to manifest low levels of iron. The assay was validated with standard reference materials and with inductively coupled plasma-mass spectrometry (ICP-MS) to accurately measure iron concentrations ∼1 × 10−6 g in about 1 × 106 cells (∼1 × 10−12 g Fe per cell). The assay requires preparation and use of a working solution to which samples can be directly added without further processing. After overnight incubation, the absorbance can be measured with a standard UV/Vis spectrophotometer to provide iron concentration. Alternatively, for expedited processing, samples can be digested with concentrated nitric acid before addition to the working solution. Optimization studies demonstrated significant deviations accompany variable digestion times, highlighting the importance to ensure complete iron ion liberation from the nanoparticle or sample matrix to avoid underestimating iron concentration. When performed correctly, this method yields reliable iron ion concentration measurements to ∼2 × 10−6 M (1 × 10−7 g/ml sample).
Numerical Modelling of Moisture Transport in Concrete under High Temperatures
Creators: name: Pohl, Christoph
Publisher: name: Zenodo
Year: 2022
Subjects: concrete
spalling
finite elements
porous media
moisture transport

description: Understanding the moisture transport inside concrete at elevated temperatures is key to understanding spalling risks. The three-phase finite element model (solid mass, dry air, water) solves the balance equations for solid phase, dry air, water vapour, liquid water and enthalpy. The model was written in FEniCS and solves the previously neglected skeleton mass balance by introducing the porosity as an additional independent variable. In addition to fulfilling the mass balance, this allows for one fewer constitutive equation, so that existing data can be used for validation. The model was experimentally validated with volumetric X-ray computer tomography data. An uncertainty quantification was performed for more informed decisions on the trust placed in the model.Variation in the input parameters were identified, the parameter space was sampled using Latin hypercube sampling and the resulting variation in the output variables determined. A numerical multiphase model allows the prediction of water content and pressures inside the pores. Solving the skeleton mass balance results in more realistic values for the skeleton mass density than previous models have exhibited. The model has shown that dehydration descriptions based on thermogravimetric analysis can not explain the moisture change seen in the CT data. The uncertainty quantification reveals a coefficient of variation of about 12% to 16% for the maximum gas pressure and its location, chosen as indicators for when and where spalling may occur. The maximum gas pressure attains approximately 7.5 MPa and occurs 50-100 mm from the exposed surface.
 Such finding is consistent with spalling experiments.
Temperature elevations can induce switches to homoclinic action potentials that alter neural encoding and synchronization
Creators: name: Hesse, Janina
name: Schleimer, Jan-Hendrik
name: Maier, Nikolaus
name: Schmitz, Dietmar
name: Schreiber, Susanne
Publisher: name: Humboldt-Universität zu Berlin
Year: 2022
Subjects: subject: neural encoding
subject: homoclinic action potentials
subject: neural synchronization
subject: action potential dynamics
subject: 570 Biologie

description: The article processing charge was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – 491192747 and the Open Access Publication Fund of Humboldt-Universität zu Berlin.
description: Almost seventy years after the discovery of the mechanisms of action potential generation, some aspects of their computational consequences are still not fully understood. Based on mathematical modeling, we here explore a type of action potential dynamics – arising from a saddle-node homoclinic orbit bifurcation - that so far has received little attention. We show that this type of dynamics is to be expected by specific changes in common physiological parameters, like an elevation of temperature. Moreover, we demonstrate that it favours synchronization patterns in networks – a feature that becomes particularly prominent when system parameters change such that homoclinic spiking is induced. Supported by in-vitro hallmarks for homoclinic spikes in the rodent brain, we hypothesize that the prevalence of homoclinic spikes in the brain may be underestimated and provide a missing link between the impact of biophysical parameters on abrupt transitions between asynchronous and synchronous states of electrical activity in the brain.
Cocaine - Molecule of the Month February 2016 [Archived version]
Creators: name: Cotton, Simon
Publisher: name: figshare
Year: 2017
Subjects: subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences

description: This is the Molecule of the Month entry for February 2016 about cocaine. It is a pdf archive version of the HTML webpage.
Melanin - Molecule of the Month March 2016 [Archived version]
Creators: name: May, Paul
Publisher: name: figshare
Year: 2017
Subjects: subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences

description: This is the Molecule of the Month entry for March 2016 about melanin. It is a pdf archive version of the HTML webpage.
Association of biofilm formation with multi drug resistance in clinical isolates of Pseudomonas aeruginosa
Creators: name: Abdulhaq, Nazia
name: Nawaz, Zeeshan
name: Zahoor, Muhammad Asif
name: Siddique, Abu Baker
Publisher: name: IfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmund
Year: 2020
Subjects: multi drug resistant
biofilm formation
Pseudomonas aeruginosa
pslA gene

description: Pseudomonas aeruginosa is considered as foremost cause of hospital acquired infections due to its innate and plasmid mediated resistance to multiple antibiotics making it a multi drug resistant (MDR) pathogen. Biofilm formation is a pathogenic mechanism harbored by this pathogen which further elevates its resistance to antibiotics and host defense system. The aim of the present study was to evaluate the biofilm forming potential and distribution of pslA gene in multi drug resistant Pseudomonas aeruginosa isolates obtained from different clinical samples. A total of 200 different clinical samples were collected after obtaining written consent from the patients. The samples were subjected to isolation and identification of P. aeruginosa by standard microbiological procedures. Confirmation of isolates was done by polymerase chain reaction targeting oprL gene. Kirby Bauer method was performed for detection of MDR isolates. Congo red agar (CRA) test and Microtiter plate assay (MPA) for observing the biofilm forming ability and amplification of pslA gene was also performed on MDR isolates. The results showed that from 200 samples 52 (26 %) were P. aeruginosa and among them 20 (38.46 %) were MDR isolates. The CRA showed 23 (44.23 %) while MPA detected 49 (94.23 %) isolates as biofilm producers while all the MDR isolates showed biofilm formation by MPA method. The pslA gene was detected in all biofilm forming isolates while 90 % in MDR P. aeruginosa. It was concluded that biofilm forming P. aeruginosa are more resistant to tested antibiotics and biofilm formation is strongly associated with presence of pslA gene.
description: EXCLI Journal; 19:Doc201; ISSN 1611-2156
Congo Red - Molecule of the Month January 2016 [Archived version]
Creators: name: May, Paul
Publisher: name: figshare
Year: 2017
Subjects: subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences

description: This is the Molecule of the Month entry for January 2016 about Congo Red. It is a pdf archive version of the HTML webpage.
Treating heart attacks with blood thinners
Creators: name: Pinto, Cathy Anne
name: Tervonen, Tommi
Publisher: name: Zenodo
Year: 2022
Subjects: Patient Preference

description: A myocardial infarction, or what is commonly known as a ‘heart attack’, happens when the large blood vessels that support the heart (known as the coronary arteries) are blocked and a part of the heart is deprived of oxygen. The blockage of your arteries is caused by a buildup of blood fats (cholesterol) and other substances in the artery walls. Without oxygen, muscle cells in the heart begin to die, which means you need to have medical treatment as soon as possible. In this study, we asked more than 300 patients about their preferences- approximately half in the acute stage and half in the chronic stage of their disease. Most of them were men, on average, 64 years old. Many of the patients also had other medical conditions, like high cholesterol, high blood pressure, or diabetes, and several of them had already used medicines that thin the blood. All patients had been hospitalized after a heart-attack, they were over 18 and living in England. We asked them about their preferences when it comes to heart attack medications. Hoping to find how much value a person gives different risks and benefits of a particular treatment, and how they balance between them.
ЗНАЧЕНИЕ ФИЛОСОФСКОГО ОБРАЗОВАНИЯ В ПРОГРЕССИВНОМ ОБЩЕСТВЕННОМ РАЗВИТИИ
Creators: name: Каримов Рахмат Рахманович
name: Бекбаев Рауф Рустамович
Publisher: name: Вопросы науки и образования
Year: 2020
Subjects: ОБЩЕСТВО
ОБЩЕСТВЕННОЕ РАЗВИТИЕ
СОЦИАЛЬНЫЙ ПРОГРЕСС
ОБРАЗОВАНИЕ
ФИЛОСОФСКОЕ ОБРАЗОВАНИЕ

description: В статье анализируется современное положение и значение философского образования, рассматриваются его особенности и актуальность в интенсивном развитии социума.
Roles of Self-Efficacy in Using Social Networking to Improve Productivity
Creators: name: Sarah Khan, Nasir Mahmood
Publisher: name: TuEngr Group
Year: 2022
Subjects: Social networking
Employee productivity
Knowledge sharing self-efficacy
Self-efficacy factor.

description: International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies, 13, 1, 13A1B: 1-11
T3.2 Translate the preliminary research questions identified in WP2 to concrete study designs
Creators: name: Veldwijk, Jorien
name: De Wit, Ardine
name: Gebel, Martin
Publisher: name: Zenodo
Year: 2022
Subjects: Patient Preference

description: WP2 sought to understand stakeholder concerns, needs and insights regarding the use of patient preference studies, aiming to recommend the use of specific methodologies for the case studies in WP3. WP3 designed, executed and evaluated the case studies of this project. Three disease areas were initially selected by PREFER for the case studies: rheumatoid arthritis (RA), neuromuscular diseases (NMD), and lung cancer. These three disease areas were selected as they span the PREFER criteria matrix for the inclusion of case studies (e.g., involve patients in different age groups, with diseases with different disease severity, treated by drugs that are relatively cheap or expensive). Besides the three case studies mentioned, PREFER aimed to add other case studies to its’ portfolio. Such studies could be led by academic or industry partners. In this task the translation of work conducted in WP2 to actual case study plans is described. Based on a developed PREFER strategy documents, GAP analysis and specified selection and review criteria, in total 11 prospective case studies were conducted and included as part of the PREFER project. This report contains synopses of these 11 PREFER case studies.
Associations between intraindividual reaction time variability and prospective memory performance in patients with schizophrenia and healthy controls
Creators: name: Liu, Jia-li
name: Chen, Tao
name: Cui, Ji-fang
name: Shi, Hai-song
name: Gan, Ming-yuan
name: Wang, Ya
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Space Science
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Neuroscience
subject: Physiology
subject: Immunology
subject: FOS: Clinical medicine
subject: Mental Health

description: Introduction: Patients with schizophrenia exhibit prospective memory (PM) impairment. Intraindividual reaction time variability (IIRTV) is an index of attentional control that is required for PM. This study examined the differences in IIRTV between patients with schizophrenia and healthy controls and the relationship between IIRTV and PM performance. Method: Thirty-nine patients with schizophrenia and forty-two healthy controls were recruited to complete a PM task and the Sustained Attention to Response Task. IIRTV was calculated as the coefficient of variation (mean/SD) of reaction time over correctly responded trials in these tasks. Results: Patients with schizophrenia showed lower PM accuracy and increased IIRTV, while the associations between PM accuracy and IIRTV were significant in healthy controls but not in patients with schizophrenia. Conclusion: These findings suggest impaired PM and relationship between PM and attentional control in patients with schizophrenia.
Evaluation of in vivo analgesic activity of Scrophularia kotscyhana and isolation of bioactive compounds through activity-guided fractionation
Creators: name: Renda, Gülin
name: Büşra Korkmaz
name: Kılıç, Merve
name: Duman, Mine Kadıoğlu
name: Kırmızıbekmez, Hasan
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Biochemistry
subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Physiology
subject: Pharmacology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: 69999 Biological Sciences not elsewhere classified

description: The present study was undertaken to evaluate the in vivo analgesic activities of the extracts prepared from the aerial parts and roots of Scrophularia kotscyhana and to isolate the bioactive metabolites from the most active extract. Analgesic activities of all extracts and subextracts at the doses of 5, 10 and 30 mg/kg (i.p.) were examined using hot plate test in mice. Among the tested extracts, MeOH extract prepared from the aerial parts and the n-butanol subextract prepared thereof displayed the best analgesic activity at all doses. Phytochemical studies on n-butanol subextract led to the isolation of two new iridoid glycosides as an inseparable mixture, 8-O-acetyl-4′-O-(E)-(p-coumaroyl)-harpagide (1) and 8-O-acetyl-4′-O-(Z)-(p-coumaroyl)-harpagide (2) along with five known secondary metabolites, β-sitosterol 3-O-β-glucopyranoside (3), apigenin 7-O-β-glucopyranoside (4), apigenin 7-O-rutinoside (5), luteolin 7-O-β-glucopyranoside (6) and luteolin 7-O-rutinoside (7). The iridoid mixture (1 and 2), 3 and 4 elicited significant inhibition of pain at 5 mg/kg dose.
Magnetic pulse welding: machine optimisation for aluminium tubular joints production
Creators: name: D. Pereira
name: J. P. Oliveira
name: T. Pardal
name: R. M. Miranda
name: T. G. Santos
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Space Science
subject: 29999 Physical Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: Medicine
subject: Pharmacology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: FOS: Biological sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Science Policy

description: Magnetic pulse welding (MPW) has recently been increasingly investigated for specific dissimilar material joining applications since this is a solid state clean process, with good energy efficiency that almost does not produce thermal degradation effects in the joint. This paper presents developments performed on a MPW machine to produce tubular joints in AA6063-T6 with lower energy than commercial machines. The welds were structural and mechanically characterised and fracture was always observed at ultimate tensile strengths above those of the base materials and outside the welded zone. An energy balance is also presented to verify the machine optimisation and the energy used compared to that reported for commercially available machines.
Serum inflammatory molecules and markers of neuronal damage in alcohol-dependent subjects after withdrawal
Creators: name: Girard, Murielle
name: Malauzat, Dominique
name: Nubukpo, Philippe
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Biochemistry
subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Neuroscience
subject: Physiology
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: 111714 Mental Health
subject: FOS: Health sciences

description: Objectives: Our aim is to describe changes in serum concentration for the pro-inflammatory factors TNF-α, IFN-γ, IL-1β, IL-8, IL-6, IL-10, IL-12 and MCP-1, for the satiety factor leptin and for factors associated with neuronal changes, neuron-specific enolase (NSE) and glial activation S100-beta protein (S100-β), and explore their association with abstinence in alcohol-dependent subjects after withdrawal. Methods: Serum sampling and clinical assessments from 115 alcohol-dependent subjects admitted to a psychiatric hospital for alcohol were repeated during the first 48 h of withdrawal (M0) and 1, 2, 4 and 6 months (M1, M2, M4 and M6) thereafter. Serum factors were determined with Luminex technology or by ELISA. Results: The levels of TNF-α, IL-1β, IL-8, IL-6, IL-12, MCP-1, and leptin decreased after withdrawal and remained low until M6, regardless of alcohol consumption. IFN-γ levels remained constant and IL-10 levels changed only slightly. NSE levels were not modified, whereas serum S100-β concentration increased significantly on M1 and then plateaued, regardless of abstinence status at 6 months. Conclusions: Alcohol-dependent subjects present an inflammatory condition that is not dependent on alcohol consumption. An understanding of the changes in concentration of the various proteins considered here would provide insight into the physiology of withdrawal or dependence. Trial registration:ClinicalTrials.gov identifier: NCT01491347.
Exploring the Relationships Between Food Insecurity, Maternal Stress and Maternal / Infant Health Outcomes During the COVID-19 Pandemic: A DOHaD Framework
Creators: name: Kalembo, Landry
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2024
Subjects: Food Insecurity
Maternal Stress
Pregnancy
COVID-19 Pandemic
Gestational Weight Gain
Birthweight

description: Adverse psychosocial, lifestyle and environmental exposures during the first 1,000 days of life can result in undesirable offspring health outcomes, including increased risk of chronic diseases in adulthood. Food insecurity (FI) and heightened stress are factors that may contribute to adverse developmental programming events, which were exacerbated for many Canadians during the COVID-19 pandemic. This study aimed to examine the relationships between maternal FI and stress during the COVID-19 pandemic and how they were associated with maternal gestational weight gain (GWG) and newborn birth weight in a Canadian pregnant cohort. Data were collected retrospectively from 273 pregnant participants of varying socioeconomic status who were pregnant and delivered infants during the pandemic (March 2020-March 2023). The participants responded to an online survey developed from previously validated questionnaires addressing FI, pandemic-related stress, self-reported GWG, and infant birth weight. Over half (55%) of the participants experienced household FI, while one fifth (20%) reported heightened stress related to pregnancy preparedness and over one third (34%) reported heightened stress related to COVID-19 infection. Participants from food secure and insecure households differed significantly in age, sexual orientation, household type, total household income, housing status, number of kids in the household and whether the pregnancy was planned or unplanned (p < 0.01). Heightened stress was also significantly associated these same factors except from age group and housing status (p < 0.05). GWG outside the recommended guidelines was reported by 77% of participants; however, no associations were observed with FI or pandemic-related stress. Infant birth weight extremes (small or large for gestational age) were reported by 32% of participants. While no associations were observed with FI status, positive associations between birth weight extremes and heightened stress related to both pregnancy preparedness and COVID-19 infection were found (p < 0.02). The results of this study provide a better understanding of the extent of FI, gestational stress, and factors potentially influencing them during the COVID-19 pandemic in a Canadian pregnant population. Recognizing the specific challenges faced by vulnerable pregnant populations can guide tailored interventions and programs aimed at improving maternal and infant health and well-being during emergency/pandemics.
Economic Consequences of Invasive Species in ornamental sector in Mediterranean Basin: An Application to Citrus Canker
Creators: name: Timpanaro, Giuseppe
name: Urso, Arturo
name: Foti, Vera Teresa
name: Scuderi, Alessandro
name: Timpanaro, Giuseppe
name: Urso, Arturo
name: Foti, Vera Teresa
name: Scuderi, Alessandro
Publisher: name: Unknown
Year: 2021
Subjects: subject: Agribusiness
subject: Agricultural and Food Policy
subject: xanthomonas citri
subject: ornamental rutaceae
subject: economic impact
subject: damage assessment with income method
subject: nurseries industry

description: Citrus Bacterial Cancer (CBC) is a severe phytopathy capable of compromising the economy, environment, and society in specific areas. To date, it is not present in the Mediterranean Basin. In essence, it could be a signal that European Phytosanitary Services have been able to control its spread, blocking import and marketing of fruits from risk areas or lacking the necessary phytosanitary requirements. However, in 2014 the EFSA launched the alarm on possible new forms of transmission of Xcc or Xanthomonas citri, the causative agent of the CBC, represented by the marketing of ornamental Rutaceae and the flow of tourism, to and from risk areas. In this context, the research carries aim to assess direct and indirect damages that an invasion of the CBC could cause to the sector: its impacts at the micro-economic level fall on the production system and the consumer, while at the macro level on the entire community. The traceability of plant material during commercial operations is proposed as a possible solution, even if it becomes an accessory burden for businesses and consumers. In this sense, this research intends to offer some useful information to public and private, interested parties and to plan intervention policies.
Leitfaden: Klausur+ – Ein strukturierter Ansatz zur Klassifikation erweiterter Prüfungsformate im digitalen Zeitalter
Creators: name: Verbarg, Julian
Publisher: name: Zenodo
Year: 2025
Subjects: Klausur+
digitale Prüfungen
hybride Prüfungsformate
Hochschuldidaktik

description: Der Leitfaden „Klausur+“ bietet eine systematische Begriffserweiterung für schriftliche Prüfungsformate im digitalen und hybriden Bildungskontext. Aufbauend auf drei Dimensionen – Raumform, Technikform und Kontrollform – ermöglicht „Klausur+“ eine präzise und anschlussfähige Klassifikation moderner Klausurformen. Ziel ist es, eine transparente Kommunikation zwischen Lehrenden, Prüfungsinstitutionen und Studierenden zu fördern sowie eine didaktisch fundierte Prüfungsplanung zu unterstützen. Der Leitfaden versteht sich als Beitrag zur Standardisierung und Weiterentwicklung differenzierter Prüfungsszenarien. Dieser Beitrag wurde verfasst von J. Verbarg, M. Sc.
CW22 Lightning Talk: Learning from An Incomplete History of Research Ethics
Creators: name: Kherroubi Garcia, Ismael
Publisher: name: Zenodo
Year: 2022
Subjects: research ethics
data ethics
history

description: An introduction to An Incomplete History of Research Ethics, a free online resource that informs data scientists and other researchers. You are invited to contribute to this open science project hosted on GitHub: https://github.com/Ismael-KG/An_Incomplete_History_of_Research_Ethics.
description: {"references": ["Ismael-KG. (2021). Ismael-KG/A-History-of-Research-Ethics: The First Ethics v0.0.1 (v0.0.1). Zenodo. https://doi.org/10.5281/zenodo.5731453"]}
Stress-Dependent SUMOylation Protects Neurons from Age Related TDP-43 Toxicity with Implications for ALS/FTD and Aging in the Mammalian Nervous System
Creators: name: Suk, Terry
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2024
Subjects: Neurodegeneration
Aging
Amyotrophic Lateral Sclerosis
Frontotemporal Dementia
TDP-43
SUMOylation
Cellular Stress
Mouse Model

description: To maintain longevity, neurons must appropriately respond to and effectively recover from environmental stressors throughout their lifespan. Disruption of these stress response pathways can lead to complex neurodegenerative diseases such as Amyotrophic Lateral Sclerosis (ALS) and Frontotemporal Dementia (FTD). The RNA binding protein TDP-43 helps facilitate appropriate stress responses through many essential roles within the nucleus. However, in ALS/FTD and conditions of prolonged stress, TDP-43 can be found aberrantly mislocalized forming large cytoplasmic aggregates causing neurotoxicity. It is critical to determine the molecular mechanisms natively regulating TDP-43 upon stress to uncover pathways that protect neurons from neurodegeneration. Covalent conjugation of SUMOs through SUMOylation has long been characterized to occur in response to cellular stress. Due to the strong overlap between roles of TDP-43 and those regulated by SUMOylation, we hypothesized that TDP-43 is likely a substrate of SUMOylation. We found that TDP-43 is SUMOylated by SUMO2/3 in a stress dependent manner at lysine 408 (K408) and is subsequently cleared through the ubiquitin-proteasome pathway upon recovery. To determine the consequences of blocking SUMOylation of TDP-43, we generated a knock-in mouse line with a TDP-43 K408R point mutation thereby preventing SUMOylation. In primary neuron cultures, we found that blocking TDP-43 SUMOylation impairs the ability for neurons to recover from stress leading to the formation of nuclear TDP-43 foci. We posited that aging may act as a stressor in vivo to elicit stress-dependent phenotypes and observed that TDP-43 K408R mice develop age-related cognitive impairment and motor neuron pathology. In human temporal lobe samples, we found that global SUMOylation is significantly upregulated during aging suggesting potentially important roles for SUMOylation beyond TDP-43 in protecting neurons in elderly individuals. Due to this global response, we question whether other targets of SUMOylation may be involved in protecting different neuron populations during aging. However, uncovering roles for SUMOylation in vivo has been a longstanding challenge due to lack of effective tools to directly compare SUMO paralogs and unbiasedly identify native substrates. Thus, we generated a His6-HA-SUMO2 knock-in mouse to complement a previously established His6-HA-SUMO1 model and demonstrated using whole brain imaging and immunoprecipitation coupled with mass spectrometry that these models facilitate the direct comparison of SUMO1 and SUMO2 distribution and paralog-specific substrates in vivo. These models will facilitate future aging studies to uncover how global SUMOylation and specific substrates maintain neuronal longevity in health and disease.
Anammox biomass carrying efficiency of polyethylene non-woven sheets as a carrier material
Creators: name: Sunja Cho
name: Minki Jung
name: Dongjin Ju
name: Lee, Young-Hee
name: Cho, Kuk
name: Okabe, Satoshi
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Biochemistry
subject: Space Science
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Inorganic Chemistry

description: To access the effects of the surface modification and fabric structure of polyethylene (PE) non-woven fabric sheets on retaining the attachment efficiency of anammox biomass, three different non-woven sheets were prepared and inserted in an anammox reactor. The hydrophobic surface modification with 10% KMnO4 and gelatin did not improve the attachment efficiency of the anammox biomass on the surface of the PE non-woven fibers. Densely packed PE-755 having the highest specific surface area to volume ratio (SA/V) (755) retained 221.4 mg biomass per unit sheet, whereas PE-181 having the lowest SA/V (181) retained only 66.4 mg biomass per unit. Accordingly, the volumetric anammox activity of non-woven sheet PE-755 was the highest among the three PE non-woven sheets because of the strong positive relationship between the specific anammox activity and biomass amount (R = 0.835, P 2 cm−3) as well as the bulk density should be considered as important parameters for the selection of non-woven biocarriers for anammox biomass.
Changing Landscapes: Farmsteads & Resort Towns
Creators: name: Baum, Sara
name: Davenport, Grace
name: Duan, Amy
name: Graham, Josette
name: Jockel, Kathleen
name: Martin, Veronica
name: Schlossenberg, Tamara
name: Tariq, Hassan
Publisher: name: Digital Repository at the University of Maryland
Year: 2019
Subjects: National Center for Smart Growth
PALS
Sustainability
Maryland
Prince George's County
School of Architecture, Planning and Preservation
Community and Social Sustainability
Economic development
Fall 2019
Paula Nasta

description: Final Project for HISP650: Historic Preservation Studio Workshop (Fall 2019). University of Maryland, College Park.
description: In the Fall semester of 2019, the University of Maryland Historic Preservation Studio class worked with the Maryland National Capital Park and Planning Commission (M-NCPPC) in Prince George’s County through the Partnership for Action Learning in Sustainability (PALS) program. The purpose of the partnership was to create a heritage trail linking the communities of Aquasco, Eagle Harbor, and Cedar Haven in southern Prince George’s County.
Codeine and Oxycodone - Molecule of the Month December [Archived version] 2015
Creators: name: Cotton, Simon
Publisher: name: figshare
Year: 2017
Subjects: subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences

description: This is the Molecule of the Month entry for December 2015 about codeine and oxycodone. It is a pdf archive version of the HTML webpage.
ВЛИЯНИЕ ПАРАЛЛЕЛЬНОГО ИМПОРТА НА ДИСТРИБЬЮТОРСТВО
Creators: name: Лобанова, Галина
Publisher: name: Zenodo
Year: 2024

description: Параллельный импорт представляет собой актуальное явление в международной торговле, в частности интеллектуальной собственности. В отличие от официального импорта, он предполагает ввоз оригинальных товаров с маркировкой товарного знака без разрешения правообладателя. Исследуются основные аспекты параллельного импорта, такие как влияние на официальные дистрибьюторы, доходы бренда, уровень сервиса и риск подделок. Автор рассматривает экономические и правовые последствия параллельного импорта для экспортирующих и импортирующих стран, анализирует различия между официальным и параллельным импортом, а также причины снижения мотивации брендов к развитию локального рынка в условиях серого импорта.
Molecular characterization of two type I acyl-CoA: diacylglycerol acyltransferase genes in maize
Creators: name: Li, Qingzhi
name: Hu, Lizong
name: Guo, Jinjie
name: Yang, Tongwen
name: Chen, Long
Publisher: name: Taylor & Francis
Year: 2016
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Genetics
subject: Molecular Biology
subject: Biotechnology
subject: Environmental Sciences not elsewhere classified
subject: Chemical Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Infectious Diseases
subject: FOS: Health sciences

description: Type I acyl-CoA: diacylglycerol acyltransferase (DGAT1) is a rate-limiting enzyme that catalyses the final step of the Kennedy pathway in triacylglycerol biosynthesis. In this study, two genes encoding type I diacylglycerol acyltransferase, ZmDGAT1a and ZmDGAT1b, were cloned from a high-oil maize inbred line and confirmed by Southern blotting. The complete coding sequences were obtained. Comparison between genomic DNA and coding sequences revealed that both ZmDGAT1a and ZmDGAT1b comprised 16 exons and 15 introns with a coding region of 1485 and 1488 bp, respectively. ZmDGAT1a and ZmDGAT1b shared similar molecular mass, isoelectric point, and transmembrane domains. ZmDGAT1b was expressed in all sampled tissues that were examined by using reverse transcription-polymerase chain reaction (RT-PCR). In contrast, ZmDGAT1a expression was limited to the silks, tassels, and seeds of different ages. This suggests that the two genes do not have a complementary relationship. The ZmDGAT1a and ZmDGAT1b proteins were subsequently found to be localized in the endoplasmic reticulum (ER). The data provides an important basis for further studies of the functions of DGAT1 genes and for genetic modification of the oil content and composition of corn seeds.
Heterogeneity of Agricultural Land Use Systems and Poverty in Sub-Saharan Africa: Relationship and Evidence from Rural Nigeria
Creators: name: Apata, Temidayo
name: Ogunleye, Kayode
name: Agboola, Olusola
name: Ojo, Tope
name: Apata, Temidayo
name: Ogunleye, Kayode
name: Agboola, Olusola
name: Ojo, Tope
Publisher: name: Unknown
Year: 2021
Subjects: subject: Community/Rural/Urban Development
subject: Food Security and Poverty
subject: Land Economics/Use
subject: Research Methods/ Statistical Methods
subject: diverse
subject: agrarian terrestrial custom schemes
subject: deprivation snare
subject: short-run sustainability index
subject: rural Nigeria

description: Several factors influencing rural-poverty in sub-Saharan-Africa, for all the factors, agricultural-land access/management and “culture of poverty” are quite dominant in literature. This study examines socio-cultural/economic factors influencing poverty and establishes linkages of heterogeneity of land-use systems. Farm-level cost–route surveys of cross-sectional national-data of 800 respondents were used for analysis. Data were analyzed by descriptive-statistics, trans-logarithmic model, and poverty-measures. Descriptive statistics depict land-ownership structure, farmer’s socio-cultural practices, and exploits of government intervention programs influenced agricultural-poverty. Trans-logarithmic coefficients results of short-run sustainability-index (SRSI), land-policy intervention variables and household-sizes are dominance factors. Also, SRSI indicated 0.69, suggesting that 69% of the farmers made unsustainable use of agricultural-land. Moreover, 92% of extremely poor respondents with large household-sizes (61.2%) seek their agricultural-land ownership by rentage, while those with land-titled documents constitute 78.6% of the non-poor. Public-policy interventions must take into account formalization of land-property rights in order to facilitate its transferability and boosting investment.
Ivermectin - Molecule of the Month October 2015 [Archived version]
Creators: name: Cotton, Simon
Publisher: name: figshare
Year: 2017
Subjects: subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences

description: This is the Molecule of the Month entry for October 2015 about ivermectin. It is a pdf archive version of the HTML webpage.
Vitamin D - Molecule of the Month November 2015 [Archived version]
Creators: name: May, Paul
Publisher: name: figshare
Year: 2017
Subjects: subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences

description: This is the Molecule of the Month entry for November 2015 about vitamin D. It is a pdf archive version of the HTML webpage.
Response letter to Fernandez-Guerra biorxiv commentary on Rodriguez del Río et al 2022 (http://disq.us/p/2n9a30u)
Creators: name: Huerta-Cepas, Jaime
name: Río, Álvaro Rodríguez Del
Publisher: name: Zenodo
Year: 2022

description: Response letter to Fernandez-Guerra biorxiv commentary on Rodriguez del Río et al 2022 (http://disq.us/p/2n9a30u)
Stevioside - Molecule of the Month September 2015 [Archived version]
Creators: name: May, Paul
Publisher: name: figshare
Year: 2017
Subjects: subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences

description: This is the Molecule of the Month entry for September 2015 about stevioside. It is a pdf archive version of the HTML webpage.
Butadiene - Molecule of the Month June 2015 [Archived version]
Creators: name: May, Paul
Publisher: name: figshare
Year: 2017
Subjects: subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences

description: This is the Molecule of the Month entry for June 2015 about 1,3-butadiene. It is a pdf archive version of the HTML webpage.
Beta-damascenone - Molecule of the Month August 2015 [Archived version]
Creators: name: Cotton, Simon
Publisher: name: figshare
Year: 2017
Subjects: subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences

description: This is the Molecule of the Month entry for August 2015 about beta-damascenone. It is a pdf archive version of the HTML webpage.
Dinitrophenol - Molecule of the Month May 2015 [Archived version]
Creators: name: Cotton, Simon
Publisher: name: figshare
Year: 2017
Subjects: subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences

description: This is the Molecule of the Month entry for May 2015 about dinitrophenol. It is a pdf archive version of the HTML webpage.
A theoretical approach to ordinal classification: feature space-based definition and classifier-independent detection of ordinal class structures
Creators: name: Bellmann, Peter
name: Lausser, Ludwig
name: Kestler, Hans A.
name: Schwenker, Friedhelm
Publisher: name: Universität Ulm
Year: 2022
Subjects: subject: ordinal classification
subject: detection of ordinal class structures
subject: Fisher’s discriminant ratio
subject: DDC 500 / Natural sciences & mathematics
subject: Classification, Broad System of Ordering
subject: Klassifikation

description: Ordinal classification (OC) is a sub-discipline of multi-class classification (i.e., including at least three classes), in which the classes constitute an ordinal structure. Applications of ordinal classification can be found, for instance, in the medical field, e.g., with the class labels order, early stage-intermediate stage-final stage, corresponding to the task of classifying different stages of a certain disease. While the field of OC was continuously enhanced, e.g., by designing and adapting appropriate classification models as well as performance metrics, there is still a lack of a common mathematical definition for OC tasks. More precisely, in general, a classification task is defined as an OC task, solely based on the corresponding class label names. However, an ordinal class structure that is identified based on the class labels is not necessarily reflected in the corresponding feature space. In contrast, naturally any kind of multi-class classification task can consist of a set of arbitrary class labels that form an ordinal structure which can be observed in the current feature space. Based on this simple observation, in this work, we present our generalised approach towards an intuitive working definition for OC tasks, which is based on the corresponding feature space and allows a classifier-independent detection of ordinal class structures. To this end, we introduce and discuss novel, OC-specific theoretical concepts. Moreover, we validate our proposed working definition in combination with a set of traditionally ordinal and traditionally non-ordinal data sets, and provide the results of the corresponding detection algorithm. Additionally, we motivate our theoretical concepts, based on an illustrative evaluation of one of the oldest and most popular machine learning data sets, i.e., on the traditionally non-ordinal Fisher’s Iris data set.
TBET‐expressing Th1 CD4+ T cells accumulate in chronic lymphocytic leukaemia without affecting disease progression in Eµ‐TCL1 mice
Creators: name: Roessner, Philipp M.
name: Hanna, Bola S.
name: Öztürk, Selcen
name: Schulz, Ralph
name: Llaó Cid, Laura
name: Yazdanparast, Haniyeh
name: Scheffold, Annika
name: Colomer, Dolors
name: Stilgenbauer, Stephan
name: Lichter, Peter
name: Seiffert, Martina
Publisher: name: Universität Ulm
Year: 2019
Subjects: subject: CLL
subject: CD4+ T cells
subject: IFNγ
subject: TBET
subject: DDC 610 / Medicine & health
subject: Leukemia, Lymphoid
subject: CD4-positive T-Lymphocytes
subject: CD4 antigens
subject: Th1 cells
subject: Chronisch-lymphatische Leukämie
subject: T-Lymphozyt
subject: Antigen CD4

description: Summary Chronic lymphocytic leukaemia (CLL) is associated with alterations in T cell number, subset distribution and function. Among these changes, an increase in CD4+ T cells was reported. CD4+ T cells are a heterogeneous population and distinct subsets have been described to exert pro‐ and anti‐tumour functions. In CLL, controversial reports describing the dominance of IFNγ‐expressing Th1 T cells or of IL‐4‐producing Th2 T cells exist. Our study shows that blood of CLL patients is enriched in Th1 T cells producing high amounts of IFNγ. Moreover, we observed that their frequency remains relatively stable in CLL patients over a time course of five years. Furthermore, we provide evidence for an accumulation of Th1 T cells in the Eµ‐TCL1 mouse model of CLL. As TBET (encoded by Tbx21) is a crucial transcription factor for Th1 polarization, we generated Tbx21−/− bone marrow chimaeric mice which showed a lower number of IFNγ‐producing Th1 T cells, and used them for adoptive transfer of Eµ‐TCL1 leukaemia. Disease development in these mice was, however, comparable to that in wild‐type controls, excluding a major role for TBET‐expressing Th1 cells in Eµ‐TCL1 leukaemia. Collectively, our data highlight that Th1 T cells accumulate in CLL but reducing their number has no impact on disease development.
Colchicine - Molecule of the Month April 2015 [Archived version]
Creators: name: Cotton, Simon
Publisher: name: figshare
Year: 2017
Subjects: subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences

description: This is the Molecule of the Month entry for April 2015 about colchicine. It is a pdf archive version of the HTML webpage.
Tagungsbericht zur Tagung in der Evangelischen Akademie Tutzing, 8. bis 10. Dezember 2017: „Annette Kolb – ihre literarische und politische Bedeutung 50 Jahre nach ihrem Tod“.
Creators: name: Leugers, Antonia
Publisher: name: theologie.geschichte
Year: 2018

description: theologie.geschichte, Bd. 13 (2018)
Machine learning-based pain intensity estimation: where pattern recognition meets chaos theory—an example based on the BioVid heat pain database
Creators: name: Bellmann, Peter
name: Thiam, Patrick
name: Kestler, Hans A.
name: Schwenker, Friedhelm
Publisher: name: Universität Ulm
Year: 2022
Subjects: subject: BioVid heat pain database
subject: Chaos theory
subject: Classification task complexity
subject: Pain intensity recognition
subject: Physiological signals
subject: DDC 000 / Computer science, information & general works
subject: Chaotic behavior in systems
subject: Decision trees
subject: Chaostheorie
subject: Entscheidungsbaum

description: In general, classification tasks can differ significantly in their task complexity. For instance, image-based differentiation between vehicles and pedestrians is most likely expected to be less complex than CT-scan-based differentiation between several lung diseases. Intuitively, based on a human point of view, one can identify some classification tasks as more complex than other classification tasks. Moreover, based on expert knowledge and/or task-specific meta information, one could attempt to estimate the complexity ranks of specific classification tasks. In this work, based on the publicly available BioVid Heat Pain Database (BVDB), we experimentally confirm the intuitive assumption that the task of automated pain intensity recognition (PIR) is very challenging. Inspired by the field of chaos theory, we show that the BVDB-specific PIR task can not only be seen as highly complex, but is even identified as a classification task of chaotic nature. To this end, we apply Hao’s working definition for chaotic systems and provide an experiment-based chaos check method. To validate our approach, as a non-complex counterpart, we include a task of handwritten numerals distinction. Our study provides two main contributions, i.e.: i) an enhanced understanding for the still present and – more importantly – substantial gap between the ground truth and the predictions reported by different research groups in combination with automated PIR tasks; and ii) an approach for a numerical complexity check based on chaos theory. Different research directions are discussed for future work. Note that improving PIR accuracy performance is not part of the study objective.