Additional file 1 of Cholesterol reprograms glucose and lipid metabolism to promote proliferation in colon cancer cells
Creators: name: Mayengbam, Shyamananda Singh
name: Singh, Abhijeet
name: Yaduvanshi, Himanshi
name: Bhati, Firoz Khan
name: Deshmukh, Bhavana
name: Athavale, Dipti
name: Ramteke, Pranay L.
name: Bhat, Manoj Kumar
Publisher: name: figshare
Year: 2023
Subjects: subject: Biochemistry
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Physiology
subject: Pharmacology
subject: Chemical Sciences not elsewhere classified
subject: Cancer
subject: Virology
subject: Computational Biology

description: Additional file 1: Supplementary figure 1. Effect of LDLc and HDLc supplementation on the proliferation of colon cancer cells.  Supplementary figure 2. Time-dependent lipid accumulation by colon cancer cells upon LDLc or HDLc treatment. Supplementary figure 3. Lipid accumulation upon LDLc and HDLc treatment in HCT-15 cells. Supplementary figure 4. Immunoblot analysis of molecules associated with colorectal cancer cells proliferation after LDLc or HDLc treatment. Supplementary figure 5. Role of LDLc and HDLc in glycolytic function, mitochondrial respiration, and ATP production in colon cancer cells. Supplementary figure 6. Role of LDLc and HDLc in the mitochondrial biogenesis in colon cancer cells. Supplementary figure 7.1. Correlation between LDLR and LDHA gene expression in colon cancer patients. Data was extracted using TMNplotter. Supplementary figure 7.2. Gene expression profile of LDLR, LDHA, MCT-4, and FASN in tumor, non-tumor and metastatic tissue of human colon from Gene CHIP Data.  Supplementary figure 7.3. Kaplan-Meier curves for survival of colon cancer patients by LDLR, FASN, LDHA, and MCT4 status. Supplementary figure 7.4. Kaplan-Meier curves for Overall survival, Relapse free survival, and Post progression survival of colon cancer patients in male and female by high and low LDLR expression. Supplementary figure 7.5. Kaplan-Meier curves for Overall survival, Relapse free survival, and Post progression survival of colon cancer patients in different stages (I-IV) by high and low LDLR expression. Supplementary figure 7.6. Kaplan-Meier curves for survival of colon cancer patients with high LDLR expression together with  high or low LDHA, FASN, and MCT-4 expression. Supplementary figure 7.7. Kaplan-Meier curves for Overall survival, Relapse free survival, and Post progression survival of colon cancer patients in male and female by high LDLR with low LDHA expression in comparison to high LDLR with high LDHA expression. Supplementary figure 7.8. Kaplan-Meier curves for Overall survival, Relapse free survival, and Post progression survival of colon cancer patients in different stages (I-IV) by High LDLR with low LDHA expression in comparison to High LDLR with high LDHA expression. Supplementary figure 8. Effect of 2-DG (2 Deoxy-D-glucose) on lipid accumulation in cells treated with LDLc and HDLc. Supplementary figure 9. Immunoblot analysis in lysates of HCT-116 cells and MC-38 tumor. Supplementary table 1. Serum parameters changes in ND, HFD, and HCD pooled mice serum. Supplementary table 2. High expression of LDLR results in poor prognosis of colon cancer patient. Supplementary table 3. Survival data of colon cancer patients expressing high LDLR with low or high LDHA expression.