title: Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð¸ Ð°Ð½Ð°Ð»Ð¸Ð· Ð¾Ð¿ÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÑ ÐµÐ¼ Ð´ÐµÑÐµÐºÑÐ¾ÑÐ° ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸ Ð´Ð»Ñ ÑÐµÐ³Ð¸ÑÑÑÐ°ÑÐ¸Ð¸ ÐÐ¦Ð Ð½Ð° Ð¼Ð¸ÐºÑÐ¾ÑÐ»ÑÐ¸Ð´Ð½Ð¾Ð¼ ÑÐ¸Ð¿Ðµ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Development and analysis of optical schemes of a fluorescence detector for PCR registration on a microfluidic chip
Creators: name: ÐÐ¾Ð±ÑÐµÑÐ¾Ð², Ð Ð¾Ð´Ð¸Ð¾Ð½
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Ð¤Ð»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ñ
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Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾-Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð°Ð½Ð°Ð»Ð¸Ð·
Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð½Ð°Ñ ÑÐµÐ¿Ð½Ð°Ñ ÑÐµÐ°ÐºÑÐ¸Ñ
Ð¼Ð¸ÐºÑÐ¾ÑÐ»ÑÐ¸Ð´Ð¸ÐºÐ°
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ÑÐ»ÑÐ¾ÑÐ¾ÑÐ¾Ñ
ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ¸Ð½
molecular genetic analysis
polymerase chain reaction
microfluidics
microfluidic chip
fluorophore
fluorescein

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description: The aim of the work is to analyze, develop and select the optical scheme of a fluorescence detector for recording a polymerase chain reaction on a microfluidic chip designed for multiplex detection of socially significant diseases. As a result of the work, the existing experimental setups and commercial devices for various methods of molecular genetic analysis were analyzed. On the basis of research and analysis, various options for optical schemes of lighting and registration systems were created and studied. Based on the results of the analysis, the most suitable scheme was selected that meets the criteria: a) the availability of the element base; b) compact design; c) the possibility of signal detection from the reaction chambers of the microfluidic chip. For the experiments, a simplified layout was assembled, which demonstrated the fundamental possibility of recording and measuring signals at fluorescein concentrations of 10^-5 M, 5*10^-5 M, 10^-4 M, and 10^-3 M. The terminological features of the subject area are taken into account and software tools are used to solve problems.