title: ÐÐ¸Ð½ÐµÑÐ¸ÐºÐ° ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÐºÐ¾ÑÐ½Ð·Ð¸Ð¼Ð° Ð¤ÐÐ Ð² Ð²Ð¾Ð´Ð½ÑÑ ÑÐ°ÑÑÐ²Ð¾ÑÐ°Ñ Ð¿ÑÐ¾Ð¿Ð¸Ð»ÐµÐ½Ð³Ð»Ð¸ÐºÐ¾Ð»Ñ Ð¿ÑÐ¸ Ð²Ð¾Ð·Ð±ÑÐ¶Ð´ÐµÐ½Ð¸Ð¸ Ð¿Ð¸ÐºÐ¾ÑÐµÐºÑÐ½Ð´Ð½ÑÐ¼Ð¸ Ð»Ð°Ð·ÐµÑÐ½ÑÐ¼Ð¸ Ð¸Ð¼Ð¿ÑÐ»ÑÑÐ°Ð¼Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Fluorescence kinetics of biological coenzyme FAD in water-propylene glycol solutions excited by picosecond laser pulses
Creators: name: ÐÑÐ°Ð´ÑÑÐ¾Ð², ÐÐ»ÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Ð¤ÐÐ
Ð¿ÑÐ¾Ð¿Ð¸Ð»ÐµÐ½Ð³Ð»Ð¸ÐºÐ¾Ð»Ñ
ÐºÐ¾Ð½ÑÐ¾ÑÐ¼Ð°ÑÐ¸Ð¸ Ð¤ÐÐ
Ð¿Ð¸ÐºÐ¾ÑÐµÐºÑÐ½Ð´Ð½ÑÐµ Ð»Ð°Ð·ÐµÑÐ½ÑÐµ Ð¸Ð¼Ð¿ÑÐ»ÑÑÑ
ÐµÑÑÐµÑÑÐ²ÐµÐ½Ð½ÑÐµ ÑÐ»ÑÐ¾ÑÐ¾ÑÐ¾ÑÑ
Ð¿Ð¾Ð»ÑÑÐ¸Ð·Ð¾Ð²Ð°Ð½Ð½Ð°Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ñ
ÑÐµÑ Ð½Ð¸ÐºÐ° TCSPC
Ð²ÑÐµÐ¼ÐµÐ½Ð° Ð¶Ð¸Ð·Ð½Ð¸ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸
Ð²ÑÐµÐ¼Ñ Ð²ÑÐ°ÑÐ°ÑÐµÐ»ÑÐ½Ð¾Ð¹ Ð´Ð¸ÑÑÑÐ·Ð¸Ð¸
ÐºÐ¾ÑÑÑÐ¸ÑÐ¸ÐµÐ½Ñ Ð°Ð½Ð¸Ð·Ð¾ÑÑÐ¾Ð¿Ð¸Ð¸
FAD
propylene glycol
FAD conformations
picosecond laser pulses
natural fluorophore
polarized fluorescence
TCSPC technique
fluorescence decay times
anisotropy coefficient and rotational diffusion time

description: ÐÐ°ÑÑÐ¾ÑÑÐ°Ñ ÑÐ°Ð±Ð¾ÑÐ° ÑÐ²Ð»ÑÐµÑÑÑ Ð¿ÑÐ¾Ð´Ð¾Ð»Ð¶ÐµÐ½Ð¸ÐµÐ¼ Ð¼ÐµÑÐ¾Ð´Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸ Ð¼Ð¾Ð»ÐµÐºÑÐ»Ñ Ð¤ÐÐ Ð² ÐÐ°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ ÐÐ¿ÑÐ¸ÐºÐ¸ Ð±Ð¸Ð¾Ð¼Ð¾Ð»ÐµÐºÑÐ» Ð¸ ÐºÐ»Ð°ÑÑÐµÑÐ¾Ð² Ð¤Ð¢Ð Ð¸Ð¼. Ð.Â Ð¤.Â ÐÐ¾ÑÑÐµ. Ð¡ÑÑÐµÑÑÐ²ÑÐµÑ ÑÑÐ´ ÑÑÐ½Ð´Ð°Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð²Ð¾Ð¿ÑÐ¾ÑÐ¾Ð², ÑÐ²ÑÐ·Ð°Ð½Ð½ÑÑ Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸ÐµÐ¹ Ð¤ÐÐ: ÐºÐ°ÐºÐ¸Ðµ ÑÐ¸Ð·Ð¸ÑÐµÑÐºÐ¸Ðµ Ð¿ÑÐ¾ÑÐµÑÑÑ ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²ÑÑÑ Ð´Ð²ÑÐ¼ Ð²ÑÐµÐ¼ÐµÐ½Ð°Ð¼ Ð·Ð°ÑÑÑÐ°Ð½Ð¸Ñ Ð¤ÐÐ, ÐºÐ°ÐºÐ¾Ð²Ð° Ð² ÑÑÐ¾Ð¼ ÑÐ¾Ð»Ñ ÐºÐ¾Ð½ÑÐ¾ÑÐ¼Ð°ÑÐ¸Ð¹ Ð¤ÐÐ Ð¸ ÐºÐ°ÐºÐ¾Ð²Ð¾ ÑÐ°ÑÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ ÐºÐ¾Ð½ÑÐ¾ÑÐ¼Ð°ÑÐ¸Ð¹ ÑÑÐ¾Ð¹ Ð¼Ð¾Ð»ÐµÐºÑÐ»Ñ Ð² ÑÐ°ÑÑÐ²Ð¾ÑÐµ? ÐÐ·ÑÑÐµÐ½Ð¸Ðµ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸ Ð¤ÐÐ Ð² ÑÐ°ÑÑÐ²Ð¾ÑÐ°Ñ Ñ ÑÐ°Ð·Ð½ÑÐ¼Ð¸ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð°Ð¼Ð¸ Ð¼Ð¾Ð¶ÐµÑ ÑÐ°Ð·ÑÐµÑÐ¸ÑÑ Ð¿ÑÐ¸Ð²ÐµÐ´ÐµÐ½Ð½ÑÐµ Ð²Ð¾Ð¿ÑÐ¾ÑÑ. Ð ÑÐ°Ð±Ð¾ÑÐµ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð»Ð¸ÑÑ Ð¾ÑÐ½Ð¾Ð²Ð½ÑÐµ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÑ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸ Ð¤ÐÐ, ÑÐ°ÐºÐ¸Ðµ ÐºÐ°Ðº Ð²ÑÐµÐ¼ÐµÐ½Ð° Ð·Ð°ÑÑÑÐ°Ð½Ð¸Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸, Ð²ÑÐµÐ¼Ñ Ð²ÑÐ°ÑÐ°ÑÐµÐ»ÑÐ½Ð¾Ð¹ Ð´Ð¸ÑÑÑÐ·Ð¸Ð¸ Ð¸ ÐºÐ¾ÑÑÑÐ¸ÑÐ¸ÐµÐ½Ñ Ð°Ð½Ð¸Ð·Ð¾ÑÑÐ¾Ð¿Ð¸Ð¸, Ð² Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾ÑÑÐ¸ Ð¾Ñ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ Ð¿ÑÐ¾Ð¿Ð¸Ð»ÐµÐ½Ð³Ð»Ð¸ÐºÐ¾Ð»Ñ Ð² Ð²Ð¾Ð´Ð½Ð¾Ð¼ ÑÐ°ÑÑÐ²Ð¾ÑÐµ. Ð ÑÐ°Ð±Ð¾ÑÐµ Ð¸Ð·ÑÑÐ°Ð»Ð°ÑÑ ÐºÐ¸Ð½ÐµÑÐ¸ÐºÐ° ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸ Ð¤ÐÐ Ð¿ÑÐ¸ Ð²Ð¾Ð·Ð±ÑÐ¶Ð´ÐµÐ½Ð¸Ð¸ ÑÐµÑÐµÐ· Ð²ÑÐ¾ÑÑÑ Ð¿Ð¾Ð»Ð¾ÑÑ Ð¿Ð¾Ð³Ð»Ð¾ÑÐµÐ½Ð¸Ñ Ð½Ð° Ð´Ð»Ð¸Ð½Ðµ Ð²Ð¾Ð»Ð½Ñ 355Â Ð½Ð¼. Ð ÐµÐ³Ð¸ÑÑÑÐ°ÑÐ¸Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸ ÑÐµÐ°Ð»Ð¸Ð·Ð¾Ð²ÑÐ²Ð°Ð»Ð°ÑÑ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÑÐµÑÐ½Ð¸ÐºÐ¸ ÑÑÐµÑÐ° ÐµÐ´Ð¸Ð½Ð¸ÑÐ½ÑÑ ÑÐ¾ÑÐ¾Ð½Ð¾Ð² Ñ ÐºÐ¾ÑÑÐµÐ»ÑÑÐ¸ÐµÐ¹ Ð¿Ð¾ Ð²ÑÐµÐ¼ÐµÐ½Ð¸. ÐÑÐµÐ´ÑÑÐ°Ð²Ð»ÐµÐ½Ñ Ð¾Ð±Ð·Ð¾Ñ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÑ Ð¿Ð¾ Ð»ÑÐ¼Ð¸Ð½ÐµÑÑÐµÐ½ÑÐ¸Ð¸ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð¼Ð¾Ð»ÐµÐºÑÐ», Ð¼ÐµÑÐ¾Ð´Ð¸ÐºÐ° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°, Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÐ° ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ, Ð° ÑÐ°ÐºÐ¶Ðµ Ð°Ð½Ð°Ð»Ð¸Ð· Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð².
description: This research is a continuation of the methodological study of FAD fluorescence in the Laboratory of Optics of Biomolecules and Clusters of the A. F. Ioffe Institute of Physics and Technology. There are several fundamental issues related to FAD fluorescence: what physical processes correspond to the two FAD fluorescence decay times, what is the role of FAD conformations in this and what is the distribution of conformations of this molecule in solution? The study of FAD fluorescence in solutions with different properties can solve these questions. The main parameters of FAD fluorescence, such as fluorescence decay times, rotational diffusion time and anisotropy coefficient, as function of propylene glycol in an aqueous solution, were experimentally studied. In this paper, the kinetics of FAD fluorescence under excitation through the second absorption band at a wavelength of 355 nm was studied. Registration of fluorescence was realized using the time-correlated single-photon counting (TCSPC) technique. This paper presents a review of the literature on the luminescence of biological molecules, the methodology of the experiment, the processing of experimental data, as well as the analysis of the results obtained.