Additional file 1 of N-terminus α-synuclein detection reveals new and more diverse aggregate morphologies in multiple system atrophy and Parkinson’s disease
Creators: name: Wiseman, James A.
name: Fu, YuHong
name: Faull, Richard L. M.
name: Turner, Clinton P.
name: Curtis, Maurice A.
name: Halliday, Glenda M.
name: Dieriks, Birger V.
Publisher: name: figshare
Year: 2024
Subjects: subject: Biophysics
subject: Biochemistry
subject: Space Science
subject: Medicine
subject: Cell Biology
subject: Neuroscience
subject: Physiology
subject: FOS: Biological sciences
subject: Pharmacology
subject: Biotechnology
subject: Environmental Sciences not elsewhere classified
subject: Computational Biology

description: Additional file 1. Supplementary Methods. Table S1. Case information for human brain tissue used in this study. Table S2. Primary antibodies used for immunohistochemistry. Table S3. Secondary antibodies used for immunohistochemistry. Table S4. Mean total pathology load (percentage of area) for each individual α-Syn antibody across PD and MSA regions. Table S5. Mean total and unique epitope-specific immunolabelling of each antibody across all regions. Figure S1. Schematic diagram summarising the quantification of total α-Syn (all epitopes), total epitope (individual α-Syn epitope), and unique epitope-specific α-Syn. Figure S2. Representative overview of the segmentation process utilised in this study. Figure S3. Representative confocal images demonstrating the morphological heterogeneity and epitope-specific α-synuclein immunolabelling of α-synuclein inclusions in the medulla, substantia nigra, hippocampus, and cerebellum of MSA-C. Figure S4. Representative confocal images demonstrating the morphological heterogeneity and epitope-specific α-synuclein immunolabelling of α-synuclein inclusions in the medulla, substantia nigra, hippocampus, and cerebellum of MSA-P. Figure S5. Immunostaining of the N-terminus, pS129, and C-terminus α-Syn antibodies in neurologically normal (substantia nigra) and MSA brain tissue (substantia nigra and medulla). Figure S6. Representative single-channel confocal images depicting the immunolabelling profile of N-terminus α-Syn (yellow), pS129 α-Syn (cyan), and C-terminus α-Syn (magenta) in glial cytoplasmic inclusions and neuronal inclusions in the human brain with MSA. Figure S7. Representative confocal images with superimposed fluorescent profiles demonstrating epitope-specific α-Syn immunolabelling in neuronal and oligodendroglial inclusions in MSA at different Z-stack levels. Figure S8. Morphological demarcation of oligodendroglial and neuronal α-synuclein inclusions by the cellular periphery. Figure S9. Distribution of α-Syn pathology (yellow) in the MSA medulla. Figure S10. Statistically significant correlations between N-terminus, pS129, and C-terminus (epitope-specific and total) immunolabelling in PD. Figure S11. Statistically significant correlations between N-terminus, pS129, and C-terminus (epitope-specific and total) immunolabelling in MSA. Figure S12. Representative single channel and merged confocal images depicting the comparable immunolabelling profile of the ab184674 (81A) and ab51253 (EP1536Y) pS129 α-Syn antibodies in both PD and MSA.