Additional file 1 of Drosophila melanogaster chemical ecology revisited: 2-D distribution maps of sex pheromones on whole virgin and mated flies by mass spectrometry imaging
Creators: name: Kaftan, Filip
name: Purva Kulkarni
name: Knaden, Markus
name: Böcker, Sebastian
name: Hansson, Bill S.
name: Aleš Svatoš
Publisher: name: figshare
Year: 2020
Subjects: subject: Biochemistry
subject: Neuroscience
subject: Physiology
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Evolutionary Biology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: FOS: Chemical sciences
subject: Ecology
subject: Developmental Biology
subject: Cardiology

description: Additional file 1: Table S1.D. melanogaster (Canton-S) recipe of 1000 mL fly food Table S2. Surface area of different body parts of six-day-old virgin female D. melanogaster (Canton-S) fly Table S3. List of compounds identified from GC-MS analysis of wings, legs and rest of the body of six-day-old virgin female D. melanogaster fly hexane extracts. (See file “Supplementary data D. melanogaster female body parts GCMS analysis.xlsx”). Figure S1. Optical and scanning electron microscopy images of six-day-old virgin female and male D. melanogaster flies. (a) Female fly freshly fixed onto a dedicated MALDI target (b-d) Female fly after 6 hours of drying in desiccator (e) Female fly after 2 days of drying (f) Male fly after 2 days of drying (g-h) Detail on male abdominal tergite deformed by drying Figure S2. Scanning electron microscopy images of six-day-old virgin D. melanogaster flies covered with LiDHB matrix using airbrush (a,b,c) and SunChrom MALDI spotter (d). (a) Female thorax (b) Female abdomen (c,d) Female wing. Figure S3. Distribution of the female sex pheromone 7,11-ND on one pair of six-day-old copulated D. melanogaster flies. Dataset was obtained by MALDI-TOF MSI in positive ion mode with the LiDHB matrix. (a) 2-D ion intensity map of 7,11-ND at m/z 411.6 ([M+Li]+ ) with signal intensity displayed in voxel view and rainbow-color coded within a mass window of ± 0.1 Da. (b) Observed mass shift of 7,11- ND represented as a 2-D ion intensity map in PlusMinus 1 color scale. (c) The mass shift correction of m/z 411.6 after applying the recalibration method. Figure S4. EI-MS chromatogram and spectra of hexane extract from 10 pairs of wings from virgin D. melanogaster females. (a) EI/MS chromatogram with focus on the retention time of female pheromones 7,11-HD and 7,11-ND. (b) Female sex pheromone 7,11-HD at m/z 376.4. (c) Female sex pheromone 7,11-ND at m/z 404.4. Figure S5. Visualization of unknown masses evincing symmetrical distribution within the region of the wing base on one six-day-old copulated female D. melanogaster fly. Dataset was obtained by MALDI-TOF MSI in positive ion mode with the LiDHB matrix. (a) 2- D ion intensity map of m/z 379.6 with signal intensity displayed in voxel view and rainbow-color coded within a mass window of ± 0.2 Da. (b) 2-D ion intensity map of m/z 419.6 with signal intensity displayed in voxel view and rainbow-color coded within a mass window of ± 0.2 Da. (c) 2-D ion intensity map of m/z 511.7 with signal intensity displayed in voxel view and rainbow-color coded within a mass window of ± 0.2 Da. Figure S6. Visualization of the female pheromones 7,11-HD and 7,11-ND within a six-day-old virgin D. melanogaster female fly wings and one set of legs using MALDI-TOF. Figure S7. MALDI-TOF MS spectrum of female pheromones 7,11-HD and 7,11-ND acquired during analysis of a last tarsal segment (with claws) of D. melanogaster female third pair of legs. Analysis was performed in profiling mode. Figure S8. EI/MS chromatogram and spectrum of hexane extract prepared by rubbing the abdomens and anogenital regions of 14 virgin D. melanogaster males using lipid-free paper. (a) EI/MS chromatogram with a focus on the elution time of cVA. (b) MS signal of male sex pheromone cVA at m/z 250.1 [M+• - CH3COOH]+•. Figure S9. Transfer of sexual pheromones cVA and CH503 from male to female during copulation was investigated via MALDI-MSI experiments with LiDHB matrix. Copulated pair of flies was analyzed on its ventral side with focus on anogenital area. Figure S10. Typical chromatogram obtained via GC-MS analysis of virgin D. melanogaster female hexane extracts.