Tylenchorhynchus mediterraneus Handoo & Palomares-Rius & Cantalapiedra-Navarrete & Liébanas & Subbotin & Castillo 2014, SP. NOV.
Creators: name: Handoo, Zafar A.
name: Palomares-Rius, Juan E.
name: Cantalapiedra-Navarrete, Carolina
name: Liébanas, Gracia
name: Subbotin, Sergei A.
name: Castillo, Pablo
Publisher: name: Zenodo
Year: 2014
Subjects: Biodiversity
Taxonomy
Animalia
Nematoda
Secernentea
Tylenchida
Belonolaimidae
Tylenchorhynchus
Tylenchorhynchus mediterraneus

description: TYLENCHORHYNCHUS MEDITERRANEUS SP. NOV.  (FIGS 4–6, TABLES 4–6)  
 Holotype: Female extracted from soil samples collect- ed from the rhizosphere of grapevine (Vitis vinifera), in Jerez de la Frontera, Cádiz, Spain, by P. Castillo, mounted in pure glycerine, and deposited in the USDA Nematode collection, Beltsville, Maryland (collection number T- 640t).   
 Paratypes: Female paratypes extracted from soil samples collected from the rhizosphere of grapevine (Vitis vinifera), in Jerez de la Frontera, Cádiz, Spain, were deposited in the following nematode collections: the USDA Nematode collection, Beltsville, Maryland (collection numbers T-6249p to T-6264p); and the Nematode collection of the Department of Nematology, Landbouwhogeschool, Wageningen, the Netherlands (collection number VIDV-5).   
 Etymology: The specific epithet refers to the geographical origin and is derived from the Latin mediterraneus = from the Mediterranean Basin.   
 Description of female: Body ventrally arcuate after fixation, no longitudinal striae or ridges outside lateral fields. Body annuli distinct but fine, 1.0–1.5 μm wide around mid-body. Lateral fields originating at the level of the conus of the stylet and extending up to hyaline region of tail to tail terminus, with four incisures, areolated throughout the body. Lip region hemispherical, offset by a constriction, with five to seven annuli; lip sclerotization weakly developed. The en face view (SEM) observations revealed a labial disc flattened laterally, dorsally and ventrally, resulting in a squarish pattern, margins of labial disc interrupted laterally by amphidial openings (Fig. 6B, C). Oral aperture slitlike, orientated dorsoventrally with three papillae on each side parallel to long axis (Fig. 6B, C). Stylet moderately strong, shaft and conus equally long; knobs rounded and posteriorly directed. DGO about 2.5 to 3 μm behind stylet base. Median pharyngeal bulb oblong to rounded, 16–18 μm long, basal bulb 55–60 μm long, pyriform or extending back over the intestine. Hemizonid usually just four to five annuli anterior to excretory pore, one to three annuli wide. Vulva a transverse slit slightly posterior to the middle of the body and distinctly protruding, most of the specimens had loose cuticle just above the vulval area. Epiptygma absent. Reproductive system amphidelphic, didelphic; anteri- or and posterior ovaries equally developed. Spermatheca rounded, filled with rounded spermatozoa. Tail conoid to cylindrical straight, with 42–81 annuli, tail terminus rounded, annulated. Phasmids located slightly anterior to middle of the tail. Postanal extension of intestine absent.   
 Description of the male: Abundant, morphologically similar to female except for sexual characters, and body in posterior region usually more curved than in female. Gubernaculum well developed, half of the spicule length. Tail terminus pointed.   
 Diagnosis: Tylenchorhynchus mediterraneus sp. nov. is characterized by a long body (850–1040 μm), a lip region hemispherical, offset by a constriction, with five to seven annuli, stylet moderately strong, 19.0–22.0 μm long with laterally to posteriorly directed rounded knobs, lateral fields with four incisures, areolated throughout the body, most of the specimens had loose cuticle just above the vulval area, tail conoid to cylindrical, straight, with 42–81 annuli, tail terminus rounded, annulated, phasmids located slightly anterior to middle of the tail, and postanal extension of intestine absent. Specific D 2– D3, ITS, and 18S rRNA sequences were deposited in GenBank with accession numbers KJ461553 – KJ461558, KJ461584 – KJ461587, and KJ461613 – KJ461616, respectively.   
*Measurements are in μm and in the form: mean ± SD (range).  
L, body length; a, body length/maximum body width; b, body length/pharyngeal length; c, body length/tail length; c′, tail length/body width at anus; V, (distance from anterior end to vulva/body length) × 100; T (distance from cloacal aperture to anterior end of testis/body length) × 100.  
*Measurements are in μm and in the form: mean ± SD (range).  
L, body length; a, body length/maximum body width; b, body length/pharyngeal length; c, body length/tail length; c′, tail length/body width at anus; V, (distance from anterior end to vulva/body length) × 100; T (distance from cloacal aperture to anterior end of testis/body length) × 100.
description: Published as part of Handoo, Zafar A., Palomares-Rius, Juan E., Cantalapiedra-Navarrete, Carolina, Liébanas, Gracia, Subbotin, Sergei A. & Castillo, Pablo, 2014, Integrative taxonomy of the stunt nematodes of the genera Bitylenchus and Tylenchorhynchus (Nematoda, Telotylenchidae) with description of two new species and a molecular phylogeny, pp. 231-264 in Zoological Journal of the Linnean Society 172 (2) on pages 236-241, DOI: 10.1111/zoj.12175, http://zenodo.org/record/5314438