Additional file 1 of Morphological, physiological, and transcriptional responses to low nitrogen stress in Populus deltoides Marsh. clones with contrasting nitrogen use efficiency
Creators: name: Chen, Cun
name: Chu, Yanguang
name: Huang, Qinjun
name: Zhang, Weixi
name: Ding, Changjun
name: Zhang, Jing
name: Li, Bo
name: Zhang, Tengqian
name: Li, Zhenghong
name: Su, Xiaohua
Publisher: name: figshare
Year: 2021
Subjects: subject: Genetics
subject: FOS: Biological sciences

description: Additional file 1: Fig. S1. Effects of low N stress on the growth traits, leaf morphology, and chlorophyll content of N-efficient (A-1, A-2, and A-3) and N-inefficient (C-1, C-2, and C-3) genotypes. Different letters above the columns indicate significant differences between groups (p &lt; 0.05). (A) Dry weight of the stem (SDW); (B) Dry weight of the root (RDW); (C) Dry weight of the leaf (LDW); (D) Chlorophyll a (Chl a); (E) Chlorophyll b (Chl b); (F) Carotenoid (Car); (G) Leaf length (LL); (H) Leaf width (LW). Fig. S2. Morphologies of the leaves (a-f) and roots roots (g-l) morphological photos of the N-efficient (a-c, g-i) and N-inefficient (d-f, j-l) genotypes responding to N limitation. N41: A-1, 141: A-2, N49: A-3; 180: C-1, N16: C-2, 5009: C-3. Fig. S3. The change trends of enzyme activities, total amino acid contents, and soluble sugar contents in leaves during N treatment of N-efficient (A) and N-inefficient (C) genotypes. T0, T1, T2, T3, T4, T5, and T6 represent 0, 3, 5, 10, 20, 30, and 40 days of N treatment, respectively. “*” indicates significant differences between LN and CK treatments in the A or C genotypes (p &lt; 0.05). (A) nitrate reductase activities (NR); (B) glutamine synthetase activities (GS); (C) glutamate dehydrogenase activities (GDH); (D) glutamine oxoglutarate aminotransferase (GOGAT); (E) Total amino acid contents (AAs); (F) Soluble sugar contents (SSs). Fig. S4. Transcriptome relationships among three biological replicates. A: N-efficient genotypes; C: N-inefficient genotypes. T0, T2, T4, and T6 represent 0, 5, 20, and 40 days of N treatment, respectively. LN: low N treatment. Fig. S5. (A) Bar chart showing numbers of upregulated and downregulated differentially expressed genes (DEGs) in the four comparison groups (T0-C vs. T0-A, T2-LN-C vs. T2-LN-A, T4-LN-C vs. T4-LN-A and T6-LN-C vs. T6-LN-A; LN: low nitrogen treatment). The magenta column shows upregulated DEGs, and the cyan column shows downregulated DEGs. (B) Venn diagram showing that the distribution of DEGs identified in the comparison of genotypes A and C are common and specific to T0, T2, T4, and T6. Fig. S6. Results of the gene ontology (GO) functional enrichment analysis of the special differentially expressed genes (DEGs) in genotypes A at different time points during the response to low N stress. (a-d) Represent the GO results of the specific DEGs at T0, T2, T4, and T6 in genotypes A, respectively. Fig. S7. (A) and (B) bar charts show the numbers of upregulated and downregulated differentially expressed genes (DEGs) in the three comparison groups of A (T0-A vs. T2-LN-A, T2-LN-A vs. T4-LN-A, and T4-LN-A vs. T6-LN-A) and C (T0-C vs. T2-LN-C, T2-LN-C vs. T4-LN-C, and T4-LN-C vs. T6-LN-C; LN: low nitrogen) genotypes, respectively. The magenta column shows upregulated DEGs, and the cyan column shows downregulated DEGs. (C) Venn diagrams showing that the distribution of DEGs identified in the comparison of different periods are common and specific to genotypes A and C. DEGs-A and DEGs-C represent all the DEGs identified from genotypes A and C during low N stress treatment, respectively. Fig. S8. Top 20 gene ontology (GO) terms of the special and common differentially expressed genes (DEGs) between genotypes A and C during the response to low N stress. (a) Represents the top 20 GO terms of the specific DEGs in genotypes A, (b) represents the top 20 GO terms of the common DEGs in genotypes A and C, and (c) represents the top 20 GO terms of the specific DEGs in genotypes C. Fig. S9. Gene expression patterns across four-time points (T0, T2, T4, and T6) in genotypes A and C under low N stress. (A) and (C) indicate the variation trend of differentially expressed genes (DEGs) in genotypes A and C, respectively. Above the box is the ID of the changing trend, and the number in the box indicates the number of DEGs contained in the trend. The grid with color indicates a significantly enrichment trend (p &lt; 0.05), and the closer the color is, the more similar the changing trend is. (B) and (D) represent the changing trend of genes in profile 12 with genotype A and profile 17 with genotype C, respectively. Fig. S10. Top 20 gene ontology (GO) terms of differentially expressed genes (DEGs) in profile 12 of genotype A (a) and profile 17 of genotype C (b). Fig. S11. Expression pattern analysis of genes in the ‘magenta’ module. Red: upregulated; blue: downregulated. A: N-efficient genotypes; C: N-inefficient genotypes. T0, T2, T4, and T6 represent 0, 5, 20, and 40 days of N treatment, respectively. LN: low N treatment. Fig. S12. Expression of key genes in nitrogen metabolism in the leaves of genotypes A and C. (A-H) represent the expression trends of NRT1;1, NRT1;2, AMT1;6, AMT2;1, NR, NiR, GS2, and GDH2, respectively. The columns represent the results of RNA sequencing, and the lines show the qRT-PCR results. Vertical bars indicate SDs (n = 3) in the qRT-PCR analysis. A: N-efficient genotypes; C: N-inefficient genotypes.