Neostagonosporella bambusicola L. S. Dissan. & J. C. Kang 2022, sp. nov.
Creators: name: Dissanayake, Lakmali S.
name: Marasinghe, Diana S.
name: Thambugala, Kasun M.
name: Kang, Ji-Chuan
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Fungi
Ascomycota
Dothideomycetes
Pleosporales
Phaeosphaeriaceae
Neostagonosporella
Neostagonosporella bambusicola

description: Neostagonosporella bambusicola L.S. Dissan. & J.C. Kang sp. nov. (FIGURE 2)
Index Fungorum number: IF559874; Facesoffungi number: FoF09712
Etymology— Referring to the host “bamboo” from which the holotype was isolated.
Holotype — HKAS115852
Saprobic on dead leaves of bamboo (Poaceae). Sexual morph: Ascostromata 180–220 × 140–180 μm (x = 201 × 163 μm, n = 10), ellipsoidal, globose to subglobose or irregular in shape, semi-immersed in host epidermis, coriaceous, solitary to gregarious, multiloculate, erumpent through host tissue, with dark brown to black, glabrous, with a centrally located ostiole. Peridium 12–20 μm wide (x = 15 μm, n = 10), thin at base, thick at sides towards apex, upper part fused with host tissue, composed of two layers; outer layer: pale to brown pseudoparenchymatous cells of textura angularis, inner layer: hyaline, somewhat flattened cells of textura angularis. Hamathecium composed of 1–3 μm (x = 2 μm, n = 10) wide, cellular, septate pseudoparaphyses, embedded in a hyaline gelatinous matrix. Asci 70–130 × 13.5–15 μm (x = 92 × 13 μm, n = 20), 8-spored, bitunicate, fissitunicate, cylindrical to cylindric-clavate, short pedicellate (up to 10 μm), apically rounded with an ocular chamber. Ascospores 30–36 × 5.5–7.2 μm (x = 33 × 6 μm, n = 20), partially overlapping uni- to bi-seriate, hyaline, cylindrical to fusiform or cylindric-clavate, with acute ends, narrower towards end cells, straight or slightly curved, 5-7-septate, slightly constricted at septa, nearly equidistant between septa, guttulate, smooth-walled, surrounded by a 10–14 μm (x = 11 μm, n = 10) thick mucilaginous sheath. Asexual morph: Undetermined.
Culture characteristics— Colonies on PDA, reaching 2 cm diam., after 2 weeks at 20°C, medium dense, circular to slightly irregular with uneven margin, slightly raised and cottony surface, colony from above: white at the margin, pale-grey at the center. From below: yellowish margin, yellow to brown at the center; mycelium pale-grey.
Material examined— CHINA, Guizhou Province, Guiyang, Guizhou University, on dead leaves of bamboo, 28 May 2019, L. S. Dissanayake (HKAS115852, holotype), ex-type culture (KUMCC 20-0031).
Known distribution — Guizhou Province, China
Notes: Neostagonosporella bambusicola shares similar morphology with N. sichuanensis showing multiloculate ascostromata and cylindrical to fusiform, transversely multi-septate, straight or curved ascospores, which are widest at the central cells. In the present phylogenetic analyses, N. bambusicola clusters sister to N. sichuanensis with relatively high statistical support (Figure 1). The two species are easily distinguishable since Neostagonosporella bambusicola has smaller ascomata (180–220 × 140–180 μm), cellular, septate pseudoparaphyses and ascospores with thick sheath (10–14 μm), whereas N. sichuanensis has large ascomata (1–2 mm × 230–340 μm), trabeculate pseudoparaphyses and ascospores with a thin sheath (5–9 μm).
description: Published as part of Dissanayake, Lakmali S., Marasinghe, Diana S., Thambugala, Kasun M. & Kang, Ji-Chuan, 2022, Neostagonosporella bambusicola sp. nov. (Phaeosphaeriaceae, Pleosporales) from bamboo in China, pp. 262-274 in Phytotaxa 573 (2) on pages 265-267, DOI: 10.11646/phytotaxa.573.2.6, http://zenodo.org/record/7349978