Metadata supporting the article: Effects of Obesity on Breast Aromatase Expression and Systemic Metabo-Inflammation in Women with BRCA1 or BRCA2 Mutations
Creators: name: Iyengar, Neil M.
name: Zhou, Xi Kathy
name: Mendieta, Hillary
name: El-Hely, Omar
name: Giri, Dilip D.
name: Lisle Winston
name: Domenick J. Falcone
name: Hanhan Wang
name: Lingsong Meng
name: Taehoon Ha
name: Pollak, Michael
name: Morrow, Monica
name: Dannenberg, Andrew J.
Publisher: name: figshare
Year: 2021
Subjects: subject: Biological Techniques
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Cancer
subject: Medicine
subject: Biomarkers
subject: 111203 Cancer Genetics
subject: FOS: Clinical medicine
subject: FOS: Clinical medicine

description: Despite evidence that estrogens are likely to play a role in the pathogenesis of breast cancer in <i>BRCA </i>mutation carriers, the effect of excess body fat on breast aromatase has not been examined in this high-risk<i> </i>population. Accordingly, a major objective of this study was to determine if excess body fat or breast white adipose tissue inflammation (WATi) are associated with increased aromatase expression in the breast microenvironment of mutation carriers. A second goal was to identify blood biomarkers that indicate elevated intra-breast aromatase levels.<br> <b> </b> <b>Data access: </b>The datasets that support the findings of this study are not publicly available in order to protect patient privacy. Data will be made available to authorized researchers who have received approval from the Memorial Sloan Kettering Cancer Center (MSKCC) Institutional Review Board. Please contact Dr. Neil Iyengar, email address: iyengarn@mskcc.org, with data access requests.<br> <b> </b> <b>Study approval and patient consent: </b>This study was approved by the Institutional Review Boards of Memorial Sloan Kettering Cancer Center (MSKCC) and Weill Cornell Medicine (New York, NY). Informed consent was provided by women undergoing mastectomy at MSKCC and breast WAT specimens were obtained under a standard tissue acquisition protocol.<br> <b>Study aims and methodology: </b>The aims of this study were: 1. to determine if excess body fat or breast WATi are associated with increased aromatase expression in the breast microenvironment of mutation carriers. 2. to identify blood biomarkers that indicate elevated intra-breast aromatase levels. The following are described in more detail in the related article: study population and biospecimen acquisition, WAT inflammation, adipocyte measurement, quantitative PCR, blood assays and biostatistical analyses.<br> <b>Datasets supporting the figures and tables in the article: </b> Data supporting figure 1: <b>BRCA clinical data NO PHI 12212018.xlsx</b>, <b>Aromatase RQ Values BRCA 1 2 Mutant.xlsx</b>, <b>Adipocyte diameters (BRCA study) format updated.xlsx.</b> Data supporting figure 2: <b>BRCA clinical data NO PHI 12212018.xlsx</b>, <b>Aromatase RQ Values BRCA 1 2 Mutant.xlsx.</b> Data supporting figure 3: <b>BRCA clinical data NO PHI 12212018.xlsx</b>, <b>Aromatase RQ Values BRCA 1 2 Mutant.xlsx</b>, <b>Leptin RQ Values BRCA 1 2 Mutant.xlsx</b> Data supporting table 1: <b>BRCA clinical data NO PHI 12212018.xlsx.</b> <b> </b> Data supporting table 2: <b>BRCA clinical data NO PHI 12212018.xlsx</b>, <b>Data Report20190709_BloodAnalytes.xlsx.</b> Data supporting table 3: B<b>RCA clinical data NO PHI 12212018.xlsx</b>, <b>Aromatase RQ Values BRCA 1 2 Mutant.xlsx</b>, <b>Adipocyte diameters (BRCA study) format updated.xlsx</b>, <b>Data Report20190709_BloodAnalytes.xlsx.</b><b><br></b>All datasets are in .xlsx file format.