Additional file 1 of Responses of soil microbial communities to freeze–thaw cycles in a Chinese temperate forest
Creators: name: Sang, Changpeng
name: Xia, Zongwei
name: Sun, Lifei
name: Sun, Hao
name: Jiang, Ping
name: Wang, Chao
name: Bai, Edith
Publisher: name: figshare
Year: 2021
Subjects: subject: Microbiology
subject: FOS: Biological sciences
subject: Ecology

description: Additional file 1: Fig. S1. Relationships between soil water content (SWC) and (a) DOC, (b) TDN, (c) TDP, and (d) NH4+-N concentration during freeze–thaw cycles. Fig. S2. Changes in nutrient content in surface water flowing through sampling plot. The c–h letters along the horizontal axis represented six sampling dates, c was the sampling date when surface runoff appeared, d and h corresponded to stage II and stage III of FTCs in this study, respectively. e, f, and g were three sampling dates between stages II and III of FTCs. More details refer to Fig. 1. Different uppercase letters indicate significant differences among different sampling dates. Fig. S3. Dynamic changes of soil (a) DOC, (b) TDP, (c) TDN, (d) DON, (e) NH4+-N, and (f) NO3−-N content during the experiment period. Different letters within the same horizon indicate significant differences among different sampling dates. Fig. S4. Dynamic changes of soil MBC content during the experiment period. Different letters within the same horizon indicate significant differences among different sampling dates. Fig. S5. Soil water-stable aggregate composition at different freeze–thaw stages. &gt; 2000 μm, large macroaggregate; 250–2000 μm, small macroaggregate; 53–250 μm, microaggregate; &lt; 53 μm, silt and clay. Fig. S6. Relative abundance of bacterial community at the genus level among different freeze–thaw stages. Different letters within the same horizon indicate significant differences among different stages for each bacterial genus. Note: Candidatus_Udaeobacter genus belongs to Verrucomicrobia phylum, HSB_OF53-F07 genus belongs to Chloroflexi phylum, IMCC26256 and Mycobacterium genus belong to Actinobacteria phylum, and Subgroup_5 genus belongs to Acidobacteria phylum. Fig. S7. A Venn diagram showing the interactions of the OTUs from different freeze–thaw stages in (a) O- and (b) A-horizon soils. Fig. S8. The relative abundance of putative bacterial functional categories based on the FAPROTAX database at different freeze–thaw stages. Different letters in the same soil horizon indicate significant differences between different stages (P &lt; 0.05). The soil horizon effect is also shown above, and red asterisks indicate positive effects and blue asterisks indicate negative effects. * P &lt; 0.05, ** P &lt; 0.01. Fig. S9 The relative abundance of putative fungal (a) trophic mode and (b) functional guilds based on the FUNGuild database at different freeze–thaw stages. Different letters in the same soil horizon indicate significant differences between different stages (P &lt; 0.05). Fig. S10. Environmental explanation of the changes in fungal abundance of the top 12 classes by RDA analysis in (a) O horizon and (b) A horizon soil. The blue arrows indicate fungal classes, and red arrows present soil properties. * P &lt; 0.05. Unrestricted permutation test, n = 999.