cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Supplemental_Appendix_1 – Supplemental material for Well-Being Interventions for Correctional Officers in a Prison Setting: A Review and Meta-Analysis
Creators: name: Evers, Trisha J.
name: Ogloff, James R. P.
name: Trounson, Justin S.
name: Pfeifer, Jeffrey E.
Publisher: name: SAGE Journals
Year: 2019
Subjects: subject: 111799 Public Health and Health Services not elsewhere classified
subject: FOS: Health sciences
subject: 160299 Criminology not elsewhere classified
subject: FOS: Law
subject: 170199 Psychology not elsewhere classified
subject: FOS: Psychology

description: Supplemental material, Supplemental_Appendix_1 for Well-Being Interventions for Correctional Officers in a Prison Setting: A Review and Meta-Analysis by Trisha J. Evers, James R. P. Ogloff, Justin S. Trounson and Jeffrey E. Pfeifer in Criminal Justice and Behavior
Powering Paths to Prevention: From Knowledge to Action with Community-led Education and Advocacy
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2025

description: Powering Paths to Prevention: From Knowledge to Action with Community-led Education and Advocacy aims to implement one of the most discussed and highest ranked breast cancer prevention intervention identified by Charting Paths to through CLASP-BC Phase 1: "Work with community groups to provide culturally tailored education and advocacy on how to reduce the disproportionate impact of breast cancer risk factors for under-resourced communities, including rural, tribal, and communities of color." In the statewide survey and eight regional meetings, participants emphasized the desire for culturally tailored, community-led education and advocacy materials to help communities understand how their priorities impact breast cancer risk and other health harms. Environmental health literacy (EHL) empowers communities to understand environmental health impacts, take individual actions to reduce their risk, and act collectively at the community level. EHL encompasses the wide range of skills needed to take environmental health information and make informed decisions, improve quality of life, reduce health risks, and protect the environment. At the CLASP-BC Phase 1 regional meetings, groups working on food security, safe bike lanes, and water quality often didn't see the link between their work and breast cancer prevention until BCPP covered the science behind the project's 15 breast cancer risk factors. Communities consistently expressed the need for this information in accessible formats. Any collective action to prevent breast cancer must start with the community understanding the impact of their risk factors. Materials must be provided in multiple languages and formats, including graphics and videos for communities with low literacy or non-written languages, and be delivered by trusted messengers. With this Planning Grant, BCPP will work with community groups, health practitioners, and academics to identify 2-3 underserved, high-risk communities that would most benefit from culturally tailored breast cancer prevention materials. We will then develop a Full Award plan to identify leaders to create Community Advisory Committees to develop breast cancer prevention materials with the research team. These resources will then be disseminated and tested for effectiveness in both educating community members and ultimately, motivating community action to reduce risk.
Supplemental Material, Online_Supplement_-_SMS_Questionnaire_Design - Questionnaire Design in Short Message Service (SMS) Surveys
Creators: name: Lau, Charles Q.
name: Sanders, Herschel
name: Ansie Lombaard
Publisher: name: SAGE Journals
Year: 2019
Subjects: subject: 160807 Sociological Methodology and Research Methods
subject: FOS: Sociology

description: Supplemental Material, Online_Supplement_-_SMS_Questionnaire_Design for Questionnaire Design in Short Message Service (SMS) Surveys by Charles Q. Lau, Herschel Sanders and Ansie Lombaard in Field Methods
Soil-based agroecological policies and measures analysis
Creators: name: Varia, Francesca
name: Galioto, Francesco
Publisher: name: Zenodo
Year: 2024
Subjects: subject: CAP policies
subject: Agroecological measures
subject: Public policies
subject: Agroecological policies
subject: Soil sciences
subject: Soil-based agroecological policies

description: This report aims to identify similarities and differences in the soil health strategies and policy instruments adopted in the seven countries participating in the INTO-dialogue project (Spain, Italy, Czech Republic, Poland, Lithuania, Latvia and Turkey). Five policy areas which are considered to be consistent with the principles of agroecology and have potential  positive impacts on the sustainable agricultural soil management have been analysed in more detail. The document therefore provides an analytical framework and insights which can be useful to support the decisions of policy makers, farmers and other key stakeholders at  both national and local levels.
Additional file 2 of Implementation of Nanopore sequencing as a pragmatic workflow for copy number variant confirmation in the clinic
Creators: name: Greer, Stephanie U.
name: Botello, Jacquelin
name: Hongo, Donna
name: Levy, Brynn
name: Shah, Premal
name: Rabinowitz, Matthew
name: Miller, Danny E.
name: Im, Kate
name: Kumar, Akash
Publisher: name: figshare
Year: 2024
Subjects: subject: Genetics
subject: FOS: Biological sciences

description: Additional file 2. Additional figures.
A multigenerational dialogue: reducing our exposures of toxic chemicals and breast cancer risk
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2025

description: Breast cancer is a complex disease. Like an onion, there are several layers that require peeling to understand the complete picture of this disease. While the disease impacts people of different races, ethnicities and cultural backgrounds, Black women are impacted the most. For example, Black women are more likely to die from the disease, they get diagnosed later, and may not have the same access to healthcare as White women. Scientists have some ideas as to why there are these differences between different groups and one of those developing ideas is related to environmental exposures from everyday use of personal care products. There are chemicals in our products that have been shown to be harmful to people’s health, including putting some people at risk of breast cancer. Because of research, we now understand that Black women and girls are exposed to these chemicals at earlier ages than other groups and these chemicals are connected to breast cancer development and advancement of this disease. Unfortunately, research information often does not reach communities quickly enough for solutions to be developed. In fact, the time gap between when research is conducted and when communities can use the information is about 17 years. The communities that would benefit the most from learning more about this information are often left out of these discussions. When Black communities are engaged in these educational efforts, we know that knowledge also increases which is a good first step towards improving people’s lives for the better. So, we—Celebrate Life Cancer Ministry (CLCM) and the Bench to Community Initiative (BCI)—are bringing together researchers, health advocates, breast cancer survivors or thrivers, community-based organizations, and media representatives to a virtual symposium to learn about these harmful chemicals and breast cancer risk with an emphasis on Black women and girls. The symposium will be on three separate days in September and people will learn about new research on this topic and receive practical skills and resources on reducing their exposures to these harmful chemicals and breast cancer risk. The speakers will be young scientists and their mentors from communities that need the information. We will also include people 13 years and older, as this important topic is for everyone that’s impacted by breast cancer.
Financial Navigation to Address Financial Toxicity among Latinas with Breast Cancer
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2025

description: Financial toxicity, or the financial hardship caused by a cancer diagnosis, is a significant concern, particularly for racial/ethnic minorities. Latina breast cancer (BC) patients living in rural areas have many challenges (e.g., language barriers, lack of knowledge, transportation) in accessing and utilizing financial resources. Financial navigation (FN), a structured intervention to assist patients in accessing financial resources, has been considered a helpful intervention. Previous studies have found that the FN program is feasible and beneficial for cancer patients, but to our knowledge, no feasibility study has been conducted with Latina BC patients in rural regions. Kern County Cancer Foundation (KCCF)-San Diego State University (SDSU) partnership aims to refine and expand the existing KCCF FN program to target Latina BC patients who live in rural areas and examine if it is feasible and effective in addressing financial toxicity. Research questions include: 1) Is the culturally targeted FN program feasible and acceptable? and 2) Will the FN program reduce financial distress and increase health-related quality of life? In Phase I, we will adapt intervention elements and materials (e.g., brochures and handouts) to fit the Latina BC patients based on feedback from three focus groups (n=24) (Aim 1). Focus group participants will discuss the clarity of information, appropriateness of the format, and layout of the materials. In Phase II, we will conduct one group pre-posttest pilot trial with 60 Latina BC patients. The FN intervention comprises 6 modules, including an introduction and FN intake module, three core service modules (e.g., financial assistance program), an education/coaching module, and an ending session. This intervention will be offered via a mobile van in rural areas. All patients will receive two weekly in-person sessions, followed by three phone calls over a period of 3 months by two full-time bilingual/bicultural financial navigators. We will assess if the FN intervention is feasible and acceptable (Aim 2) and if the intervention reduces financial distress and increases health-related quality of life (Aim 3). This community-partnered participatory research approach can effectively address the cancer community's needs regarding financial toxicity and enhance community capacity to sustain the FN program.
GPIR-18-082.R2_Supp – Supplemental material for Political conservatism and the exploitation of nonhuman animals: An application of system justification theory
Creators: name: Dhont, Kristof
name: Hodson, Gordon
name: Loughnan, Steve
name: Amiot, Catherine E.
name: Hoffarth, Mark R.
name: Azevedo, Flávio
name: Jost, John T.
Publisher: name: SAGE Journals
Year: 2019
Subjects: subject: 170199 Psychology not elsewhere classified
subject: FOS: Psychology

description: Supplemental material, GPIR-18-082.R2_Supp for Political conservatism and the exploitation of nonhuman animals: An application of system justification theory by Kristof Dhont, Gordon Hodson, Steve Loughnan, Catherine E. Amiot, Mark R. Hoffarth, Flávio Azevedo and John T. Jost in Group Processes & Intergroup Relations
Additional file 1 of An eHealth symptom and complication management program for cancer patients with newly created ostomies and their caregivers (Alliance): a pilot feasibility randomized trial
Creators: name: Xu, Shenmeng
name: Tan, Xianming
name: Ma, Chunxuan
name: McElyea, Rebecca S.
name: Shieh, Karl
name: Stover, Angela M.
name: Smith, Angela
name: Stitzenberg, Karyn
name: Basch, Ethan
name: Song, Lixin
Publisher: name: figshare
Year: 2024
Subjects: Medicine
Biotechnology
Biological Sciences not elsewhere classified
Science Policy

description: Additional file 1.
Tobacco exposure as a breast cancer risk factor among Vietnamese American women in California
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2025

description: Our team proposes to examine the impact of tobacco exposure, secondhand smoke exposure (SHSe) in particular, and its link to breast cancer risk among Vietnamese American (VA) women. Women who have been exposed to SHSe are more likely to develop breast cancer than women unexposed to SHSe. More VA men tend to smoke cigarettes than other groups, placing VA women at high risk for SHSe. Despite the prevalent SHSe among VA women, there is a gap in research on this topic with regards to the cultural, historical and familial contexts within the VA community. Thus, our study seeks to explore multilevel determinants of SHSe as a breast cancer risk factor among VA women. Specifically, the proposed study will identify cultural, neighborhood, family dynamics, and community norms in relation to SHSe within the VA community. Based on the findings, the ultimate goal is to develop culturally appropriate strategies to reduce tobacco exposure and improve breast health among VA women.
Impact of feminine hygiene products on Black women's body burden of breast cancer-related chemicals
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2025

description: Introduction. Non-menstrual feminine hygiene products (FHPs), including douches, wipes, and sprays, can contain multiple chemicals that induce genetic mutations and disrupt hormones. Use of FHPs on highly permeable genital skin may contribute to breast cancer risk more than other personal care products. Black women, who have the highest breast cancer mortality rate, also have the highest use of FHPs. Our long-term goal is to conduct an intervention study to reduce urinary concentrations of 1000+ breast cancer-related chemicals (BCRCs) in 200 Black participants by replacing common FHPs with lower toxicity alternatives. Using CRC pilot funding, we will form a community-research partnership to conduct comprehensive chemical analyses of common FHPs and lower toxicity alternatives; compare results to ingredients lists; develop participant surveys; and obtain ethics approval for the full study. Research questions. 1) What are the most commonly used FHPs on the market and what lower toxicity alternatives exist? 2) What is the true chemical make-up of these products and alternatives and how do they deviate from listed ingredients? 3) How can we most effectively frame and structure our participant survey questions to gather information on their current use of feminine hygiene products and other relevant information? General methodology. We will establish a community-research partnership to collaborate with stakeholders and the community at large on research design, implementation, and dissemination. We will conduct analyses capable of detecting 200+ chemicals on a diverse set of 25 commonly used FHPs plus 25 alternatives with no listed BCRC ingredients for analyses. We will compare analytical results with the products’ ingredients lists and describe breast cancer-relevant exposures. We will develop participant surveys to be administered before and after the intervention period to capture current FHP use, demographics, and other relevant information. Innovative elements. The contribution of FHPs to breast cancer risk has not yet been explored. Our proposed project will be the first to conduct an intervention study on the effect of FHP use on BCRC biomarkers or to use comprehensive chemical analyses on a set of FHPs. It will be the first project funded by CBCRP to focus on FHPs.
Breast Cancer in Young Women: Awareness, Views, Experiences (BRAVE) Study
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2025

description: Breast cancer is the most common cancer and a leading cause of cancer death for women under age 40 (i.e., young women). The rate of breast cancer has also been rising in young women, which has not been observed in older women. Studies have shown young women are more likely to be diagnosed with later staged, more aggressive tumors and have poorer survival. To improve breast cancer outcomes in young women, timely diagnosis is imperative, however young women have been found to be more likely to experience delays in their breast cancer diagnosis. Currently, no study has examined the underlying reasons for these delays in young women in the U.S. We are proposing to conduct the Breast Cancer in Young Women: Awareness, Views, Experiences (BRAVE) Study to understand what contributes to a timely breast cancer diagnosis for young women and providers and to determine how our study’s findings can inform efforts for timely diagnosis in young women. To address these objectives, we will conduct focus groups of women who were diagnosed with breast cancer under age 40 within the past 10 years in California to capture their pre-diagnostic experiences, including their awareness, attitudes, and behaviors leading up to their diagnoses. We will also conduct in-depth, one-on-one interviews with healthcare providers who routinely see young female patients in their California-based practice to capture each provider’s perceptions, approach, and procedures to diagnosing a young woman with breast cancer. These focus groups and interviews will be recorded, transcribed, and analyzed to identify thematic patterns. We will then share the study’s findings by presenting them to our Community Advisory Board, which will include well-respected researchers, providers, and survivors, as well as organizing a Data Walk event for study participants, community members, and relevant stakeholders. These dissemination plans will help the community partner better understand how to improve timely diagnosis of breast cancer in young women. Given that young women are often overlooked when it comes to breast cancer preventive care and the young breast cancer survivor community is understudied, this project will raise awareness that young women get breast cancer too and help the community partner and their efforts to promote timely diagnosis of breast cancer in future young women.
East Oakland's 40x40: Building integrative place-based approaches to achieve health equity
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2025

description: Structural racism works through institutions, laws, policies, norms, and beliefs to advantage the White majority and disadvantage racial minority groups, including African Americans. It can function in communities by restricting access to benefits (e.g., quality education, housing, healthcare) or by exposure of residents to more incarceration or air pollution. Due to its broad impact on conditions that hinder good health, structural racism is a fundamental cause of poor health among racial minority groups. It has been linked to later breast cancer stage at diagnosis and worse survival for Black females. Oakland, California has historically been a location of segregation and structural racism for African Americans. More recently, most areas of Oakland have undergone gentrification and displacement of African American residents. Deep East Oakland remains the last community that is home to many long-time African American residents, but it is now at risk for resident displacement. Our work will be grounded in the people of East Oakland to support the community’s objectives to counter its history of structural discrimination and the threat of gentrification and displacement of residents. Through this proposed planning project, we aim to partner with the 40x40 initiative and the People’s Advisory Council in East Oakland. The 40x40 initiative is a coalition community organizations, African American leaders, residents, and businesses. A key aspect is the People’s Advisory Council comprised of community members and 40x40 representatives. Our study team will partner with the council to design a community-based research study that addresses a community need. First, we will partner with the People’s Advisory Council to identify one or more areas of structural need within East Oakland that aligns with our team’s expertise. Second, we will design an adaptation of a mobile mapping platform (Streetwyze) so that we can collect crowd-sourced data from East Oakland residents to assure broad applicability of an intervention in the community. Our third aim is to develop a proposal to design, implement, and test an intervention targeting that area of need. We hope our work will provide East Oakland with additional knowledge, tools, and connections to protect and build their community to support the health of its residents, including Black females at risk of breast cancer.
TEACHING THE UZBEKI LANGUAGE USING METHODS
Creators: name: Palvonnazarova, Hayatkhan
Publisher: name: Zenodo
Year: 2024

description: The article discusses the issues of teaching spelling rules to students based on the cacography method, that is, the method of error justification, correcting texts containing deliberately introduced errors. Various methods and approaches used in teaching the Uzbek language, from traditional methods to modern pedagogical strategies, are considered.
Investigating the biogenesis of histone mRNAs during normal and aberrant cell proliferation
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2025

description: Regulation of histone biosynthesis during the cell cycle is critical for coordinating chromatin assembly, DNA replication, and gene transcription. Overproduction or underproduction of histone protein can result in replication stress and genome instability that contribute to the development of cancer. The supply of histone proteins is primarily regulated at the level of replication-dependent (RD) histone mRNA production, a process that is tightly coupled to the phase of the cell cycle but remains incompletely understood. Here, we propose to investigate the mechanism of RD histone mRNA transcriptional termination, an enigmatic aspect of histone biogenesis with particular relevance to cancer formation. RD histone mRNAs are the only eukaryotic mRNAs in animal cells that lack poly(A) tails, ending instead with a conserved stem-loop structure. The genes encoding all five RD histone proteins are clustered in metazoan genomes, and transcription and pre-mRNA processing factors required for histone mRNA biosynthesis are organized into a nuclear body (the histone locus body or HLB) that assembles at these gene clusters. Because of their close proximity to one another, transcription of each RD histone mRNA copy has to terminate shortly after the stem-loop; delayed termination akin to that seen with most polyadenylated mRNAs can lead to aberrant replication stress, aberrant gene transcription, and chromosomal instability, which are signature hallmarks of cancer. In our preliminary studies, we found that acute depletion of RNase MRP - a ubiquitous and essential endonuclease known largely for its critical role in maturation of rRNAs – specifically affects termination of RD histone mRNA transcription. Here, we propose to investigate the requirement for the endonuclease activity of RNase MRP in RD histone mRNA termination and delineate the direct and indirect mechanisms by which compromised activity of RNase MRP could lead to deregulated production of histones and cancer development. This study will address one of the least understood aspects of histone gene regulation and shed light on the elevated risk of cancer development in patients with mutations in RNase MRP.
Defining mechanisms of YAP-driven high-grade complex karyotype sarcoma development
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2025

description: Sarcomas are a rare, heterogeneous, and understudied group of connective tissue cancers with varying genetics, histologies, and patient demographics. Within sarcomas, few therapeutic targets have been identified and those tested in clinical trials demonstrate limited efficacy. As a result, people with sarcoma have a 65% five-year survival rate despite aggressive multimodality treatment with surgery, chemotherapy, and radiation. Increasing our understanding of sarcoma biology, discovering new therapeutic vulnerabilities, and identifying opportunities for targeted therapies will improve patient outcomes. We have developed a robust pre-clinical model that allows for the study of sarcoma development, growth, and metastasis across complex karyotype subtypes. Using this system, we identified oncogenic drivers of sarcoma development including Yes-associated protein (YAP). YAP amplification is observed across human sarcomas and was also observed in our pre-clinical model. YAP is a transcriptional regulator of the Hippo pathway that, in conjunction with coactivators controls development, differentiation, and cancer in context and cell specific manners. During the development of YAP driven tumors, we observed increased activity of Enhancer of Zeste 2 (EZH2). This protein is a key component of the polycomb repressor complex 2 (PRC2) that methylates histones at H3K27 and recruits methyltransferases resulting in a closed chromatin state. We hypothesize that EZH2 is required for the development and progression of YAP driven sarcomas. In this study, we will define the interaction between YAP and EZH2 during transformation and determine if therapeutic targeting of YAP and EZH2 is a feasible clinical strategy. The era of targeted treatments and immunotherapy ushered in significant improvements in overall survival for patients with cancer. Despite this, the prognosis of high-grade complex karyotype sarcomas has remained stagnant. Inhibitors of YAP and Hippo signaling are under development and in clinical trials. Tazemetostat, an inhibitor of EZH2 is FDA approved for epithelioid sarcoma and follicular lymphoma. This study will be the first to investigate the role of YAP and EZH2 in complex karyotype sarcomas and validate these as therapeutic targets. The pre-clinical data from this study will inform future clinical trials for this aggressive and heterogenous tumor type.
Targeting RNA splicing in B-cell acute lymphoblastic leukemia
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2025

description: The proposed project is built upon our prior transcriptomic studies that led us to a new area of research studying the role of RNA splicing in cancer. SF3B1 is a core component of the spliceosome and an essential protein in the RNA splicing process. Mutations in SF3B1 are a common cause of RNA dysregulation in multiple carcinomas and myeloid malignancies, and targeting the spliceosome has been evaluated in preclinical models and clinical trials. On the other hand, aberrant expressions, not mutations, of SF3B1 are common in cancers, including sarcomas and lymphoid malignancies, suggesting the role of RNA splicing can be different in different cancers. Recently a study on aberrant RNA splicing and its potential as a therapeutic target in T-cell acute lymphoblastic leukemia was published. Aberrant RNA splicing has also been reported in B-cell acute lymphoblastic leukemia (B-ALL); however, its role and therapeutic potential are not yet known. Our preliminary studies in B-ALL suggest that leukemia-initiating cells (LICs) that we discovered use unique RNA splicing as a survival mechanism and are sensitive to SF3B1 targeting. Therefore, targeting leukemia cells, including LICs, has the potential to eradicate leukemia at its root. LICs are believed to be the reason for disease relapse, and relapsed diseases still have very poor outcomes. Our project has the potential to overcome the significant challenges of treating relapsed diseases. We hypothesize that SF3B1 selectively regulates apoptosis-associated genes, and inhibition of SF3B1 induces cell death in B-ALL cells. We will investigate the role of SF3B1 in B-ALL cell survival, particularly in LICs. We will investigate the therapeutic potential of SF3B1 inhibition, with or without another drugs, both in vitro and in vivo, using our well-established, patient-derived high-risk B-ALL xenograft models. Understanding the role of RNA splicing in leukemia cell apoptosis in this project has the potential to lead to novel therapeutics to improve overall outcomes by decreasing treatment failure and relapse and has the potential to minimize negative side effects of current treatments by directing the therapy to LICs. Adolescents and young adults with B-ALL have particularly poor outcomes.
3.Supplementary_information-2 – Supplemental material for Synthesis of C-ring hydrogenated sinomenine cinnamate derivatives via Heck reactions
Creators: name: Pan, Hongmei
name: Lu, Tong
name: Wu, Xuedan
name: Jiang, Chuan
name: Gu, Chengwen
name: Zhang, Kehua
name: Jin, Jie
Publisher: name: SAGE Journals
Year: 2019
Subjects: Materials Engineering not elsewhere classified

description: Supplemental material, 3.Supplementary_information-2 for Synthesis of C-ring hydrogenated sinomenine cinnamate derivatives via Heck reactions by Hongmei Pan, Tong Lu, Xuedan Wu, Chuan Jiang, Chengwen Gu, Kehua Zhang and Jie Jin in Journal of Chemical Research
Targeting Oncogene-Induced Replication Stress for Neuroblastoma
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2025

description: Children with high-risk and relapsed neuroblastoma (NB) need improved therapies, and recurrent cytogenetic abnormalities, such as amplification of the MYCN oncogene, represent candidate therapeutic targets. MYCN amplification is associated with significantly worse survival rates for children with NB, and MYCN amplifications in NB can be found both within the linear genome and on circular extrachromosomal DNA (ecDNA). Amplifications of additional oncogenes in NB tumors, such as CDK4 and MDM2, have also been found on ecDNA, suggesting a broad role for ecDNA in NB pathogenesis. MYCN amplification via ecDNA has been linked to the presence of replication stress (RS), and RS is associated with defects in DNA replication and repair and is an important driver of tumor initiation and progression via induction of chromosomal instability. However, the role of RS and its significance as a potential therapeutic target in NB are unknown. We have established that MYCN-amplified NB cells demonstrate significantly increased sensitivity to inhibition of targets that generate increased RS compared to NB cells without MYCN amplification. We hypothesize that enhanced sensitivity of MYCN-amplified NB cells to induction of RS is due to a lethal increase in levels in RS beyond that induced by ecDNA formation and maintenance. Therefore, we propose to evaluate NB cells and tumors for the mechanisms of vulnerability to RS induction. We will investigate the contributions of MYCN amplification and expression and of ecDNA prevalence and content to sensitivity to RS induction in NB and evaluate the roles of MYCN- and ecDNA-induced RS in NB cell and tumor growth and response to treatment. Our proposed research will identify and validate novel approaches for targeting MYCN in NB via the unique strategy of targeting RS in a cancer cell-specific fashion. The proposed research will also facilitate future clinical trials and commercialization of novel inhibitors capable of inducing RS for treating MYCN-amplified NB tumors in need of less toxic and more effective therapies.
Control of cell cycle entry
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2025

description: The decision to enter a new round of cell division is amongst the most consequential decisions in the life of a cell. Entry into the cell cycle occurs only when sufficient growth has occurred and only when there are sufficient nutrients for further growth. In animal cells, cell cycle entry is controlled by growth factors, which ensures that cell division occurs at an appropriate time and place. Defects in the signals that control cell cycle entry are a primary cause of cancer. The mechanisms that initiate cell cycle entry are poorly understood. The critical molecular event that drives cell cycle entry is expression of late G1 phase cyclins. Decades of work in both yeast and mammals led to a canonical model in which initiation of cell cycle entry occurs entirely via activation of late G1 phase cyclin transcription. In this model, a cyclin expressed in early G1 phase activates a cyclin-dependent kinase (Cdk), which then phosphorylates and inactivates a repressor protein that inhibits transcription of late G1 phase cyclins. In mammalian cells the repressor is the tumor suppressor Rb. In yeast, the repressor is Whi5. This model has been highly influential and has guided the field for decades, despite the existence of numerous observations that are difficult to reconcile with the model. Moreover, recent tests of the model in both yeast and mammalian cells have shown that the model is inadequate and inaccurate. The critical molecular events that initiate cell cycle entry remain largely unknown, which means that the mechanisms by which oncogenic signals influence cell cycle entry also remain unknown. The goal of our work is to discover conserved molecular mechanisms that control cell cycle entry, which could lead to transformational breakthroughs in our understanding of how cell cycle entry is initiated, how it is influenced by oncogenic signals, and how cancer cells develop resistance to new drugs that target cell cycle entry.
Estudo de Viabilidade Socioeconômica para implantação do curso de ciência de dados na UFRRJ no município de Três Rios.
Creators: name: Paulo José Saraiva
name: Teofilo de Paula
name: Ferreira Filho, Diogenes
Publisher: name: Zenodo
Year: 2023

description: Estudo de Viabilidade econômica para implantação de curso de ciência de dados no município de Três Rios.
LINCing macromolecular crowding and pancreatic ductal adenocarcinoma
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2025

description: Nucleoplasmic macromolecular crowding (MMC) is a critical yet often overlooked factor in cancer progression. This proposed research is the first to consider how MMC influences progression in pancreatic ducal adenocarcinoma (PDAC). PDAC patients have a 5-year survival rate below 5%. While the PDAC is primarily driven by genetic mutations in KRAS and tumor suppressor genes, PDAC metastasis lacks recurrent genetic drivers. This suggests that non-genetic factors play a critical role in shaping the disease’s progression. Research shows a relationship between MMC and progress in other cancers. We propose investigating how nucleoplasmic MMC influences PDAC progression and metastasis using innovative tools like nucleoplasmic genetically encoded multimeric nanoparticles and 3D PDAC organoid models. Our observations hint at perturbed nucleoplasmic MMC in metastatic PDAC organoids, suggesting a potential role in PDAC metastasis. Furthermore, analysis of existing PDAC organoid RNA-seq datasets has unveiled the up-regulation of nesprin-3, a crucial component of the outer nuclear membrane and the linker of nucleoskeleton and cytoskeleton (LINC) complex, in metastatic PDAC organoids. LINC complexes govern cytoplasmic MMC, nuclear-cytoplasmic transport, mechanotransduction, and chromatin organization. Our proposed experiments will determine whether nesprin-3 regulates nucleoplasmic MMC, offering a promising avenue for targeted therapy against PDAC metastasis. We will address two specific aims: 1) Do changes in nucleoplasmic MMC correlate with PDAC prognosis in 3D pancreatic organoid models? and 2) Do alterations in nucleoplasmic MMC affect PDAC cell metastatic capabilities in vivo? This proposal capitalizes on the synergy of an interdisciplinary collaboration between a developmental cell biologist (Daniel A. Starr), a quantitative cellular biophysicist (G.W. Gant Luxton), and an expert in PDAC and organoid modeling (Chang-Il Hwang). By bridging the gap between genetic and non-genetic factors, our study will provide comprehensive insights into the biophysical properties of the nucleoplasm and their influence on the aggressive traits of PDAC. The potential identification of a novel therapeutic target holds immense promise for addressing the daunting challenge of metastasis in PDAC patients, offering hope for improved clinical outcomes and enhanced quality of life.
title: Real-Time Measurements on the Effects of Ultra-Short High Voltage Pulses on HL-60 Cells
Creators: name: Artemiou, Michalis C.
Publisher: name: Old Dominion University
publisherIdentifier: https://ror.org/04zjtrb98
publisherIdentifierScheme: ROR
Year: 2004

description: The application of high voltage microsecond pulses that are above a critical value, to biological cells, has been shown to cause electroporation (Neumann, 1989). In addition, ultra-short pulses of several tens to hundreds of nanoseconds duration have been recently shown to affect intracellular structures without disturbing the plasma membrane (Schoenbach, 2001). However, such ultra-short intense pulses are expected to cause deformation of cells due to Maxwell stresses. This deformation was studied by means of an imaging technique with 10 ns temporal resolution on HL-60 cells. The imaging system consists of an inverted microscope where a spark light source was used to illuminate the sample. The microscope slide, having two stainless steel electrodes with a gap distance of 100 μm, is placed under the microscope. The electrodes are connected to a Blumlein generator, which generates high voltage pulses 10, 60 and 300 ns long. With a load resistance of 100 Ω , the impedance of the pulse forming line is matched to the resistance of the cells in suspension between the electrodes, generating a nearly rectangular pulse. This pulse can be applied with a maximum voltage of 850 V, corresponding to an electric field of 85 kV/cm. To achieve the desired temporal resolution, we use a high intensity nanolamp with a peak output power of 500 kW, which emits a white spectrum flash of light for only 10 ns. Pulse generator and nanolamp are synchronized with a CCD camera attached to the microscope. Light flash and electric pulse can then be shifted in time against each other as needed, to observe various effects. It was found that pulsed electric fields (PEFs) of 85 kV/cm with 300 ns duration, caused two different effects depending upon the medium of the cell suspension. In low conductivity media, HL-60 cells underwent elongation in the field direction. The horizontal axis increased up to 5.4%, whereas the vertical axis decreased by 2.1%. In higher conductivity or physiological media under the same conditions, HL-60 cells showed negligible deformation effects.
Identifying cancer-associated fibroblasts in glioblastoma and defining their pro-tumoral effects
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2025

description: Glioblastoma (GBM) is a devastating brain tumor lacking effective treatments. Recent evidence has suggested that the growth and treatment refractoriness of GBM derives in large part from the dynamic interplay between tumor cells and supportive cells in the tumor microenvironment. Along those lines, my lab has intriguing data using single-cell RNA-sequencing to identify cells in GBM that express canonical cancer-associated fibroblast (CAF) markers, have CAF morphology, and transcriptomically resemble CAFs from other cancers rather than other stromal cells with similar surface markers such as pericytes. These findings challenge conventional thinking because, while the existence of CAFs in many systemic cancers and their pro-tumoral effects were demonstrated over two decades ago, CAFs had been assumed to be absent in GBM given the lack of normal fibroblasts in the healthy brain. Using spatial transcriptomics, we also demonstrated that these CAFs are enriched in the perivascular niche (PVN) of GBM close to GBM stem cells (GSCs), where they increase GSC proliferation. GBM CAFs also promote pro-tumoral effects on macrophages in GBM. Here, we will build on this intriguing data by investigating our central hypothesis that the GBM PVN is a unique regional microenvironment in which GSCs recruit circulating marrow-derived progenitors that differentiate into CAFs that alter GSCs and their microenvironment in a pro-tumoral manner that promotes therapeutic resistance. We will investigate this hypothesis through three aims: Aim 1 – Determine the origin of CAFs in GBM and the mechanism of their recruitment to the GBM perivascular niche; Aim 2 - Define the immunosuppressive effects of CAFs on T-cells in the GBM microenvironment and the mechanisms of these effects; and Aim 3 - Characterize the role of CAFs in GBM resistance to standard cytotoxic therapies. We will use transcriptome-based lineage trajectory prediction algorithms and murine transplantation models to define the lineage of GBM CAFs, functional immune cell assays to assess the impact of GBM CAFs on immune cells in the microenvironment, and mouse models to delineate the impact of CAFs on GBM therapeutic resistance. Our work will challenge conventional thinking by showing that CAFs exist in GBM, where they are not merely bystanders but active participants in the growth and treatment refractoriness of the tumor.
MPEC 2025-E84 : 2025 EH1
Creators: name: Minor Planet Center Staff
Publisher: name: The Minor Planet Center
Year: 2025
Subjects: subject: Small Solar System bodies
valueUri: http://astrothesaurus.org/uat/1469
subject: 2025 EH1

description: The Minor Planet Electronic Circulars contain information on unusual minor planets, routine data on comets and natural satellites, and occasional editorial announcements.  They are published on behalf of Division F of the International Astronomical Union by the Minor Planet Center, Smithsonian Astrophysical Observatory, Cambridge, MA 02138, U.S.A.
sj-pdf-1-ipe-10.1177_2041669520913319 - Supplemental material for “The Harder One Tries …”: Findings and Insights From the Application of Covert Response Pressure Assessment Technology in Three Studies of Visual Perception
Creators: name: Tsikandilakis, Myron
name: Bali, Persefoni
name: Haralabopoulos, Giannis
name: Derrfuss, Jan
name: Chapman, Peter
Publisher: name: SAGE Journals
Year: 2020
Subjects: subject: 110904 Neurology and Neuromuscular Diseases
subject: FOS: Clinical medicine
subject: 170199 Psychology not elsewhere classified
subject: FOS: Psychology
subject: 110319 Psychiatry (incl. Psychotherapy)
subject: Neuroscience

description: Supplemental material, sj-pdf-1-ipe-10.1177_2041669520913319 for “The Harder One Tries …”: Findings and Insights From the Application of Covert Response Pressure Assessment Technology in Three Studies of Visual Perception by Myron Tsikandilakis, Persefoni Bali, Giannis Haralabopoulos, Jan Derrfuss and Peter Chapman in i-Perception
The evolution of cancer in planarians
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2025

description: Arguably, one of the least understood aspects of cancer is how normal cells acquire cancer features. This process of malignant transformation endows some cells with extraordinary properties to avoid surveillance mechanisms, skip cell death, and proliferate indefinitely. Most cases of cellular transformation occur as tissues replace old and damaged cells with new ones. However, tissue renewal is challenging to analyze as it tends to happen simultaneously in many tissues that turnover at different rates for many years. In humans, for example, cellular turnover is continuous throughout the lifespan, deploying billions of cells daily to renew tissues such as blood, epidermis, and gastrointestinal epithelia. A significant limitation in the field is the lack of experimental models to analyze systemic tissue renewal and malignant transformation simultaneously. A predominant view explaining the progression of malignancy claims that the deregulation of tumor suppressors induces DNA damage that is present at the early stages in almost all cancers. We propose a paradigm shift by introducing a simplified experimental model to enable in situ analyses of cellular transformation during tissue renewal. The planarian flatworm continuously renews about 40 tissue types throughout the body, and we developed the first planarian cancer model by inhibiting PTEN, the second most inactivated gene in human cancers. PTEN-mediated cancer in planarians aggressively elicits a phenotype that resembles mammalian carcinogenesis. It takes less than two weeks to observe neoblast over-proliferation, tissue invasion, and the formation of lethal tumors. However, how malignancy arises and evolves during tissue renewal in planarians is unknown. We aim to leverage the integration of systemic tissue turnover and PTEN function to address the earliest manifestations of malignancy and identify the cells driving the cancer phenotype. Preliminary results demonstrate that DNA damage underlies cellular transformation that is detectable after a few hours of PTEN inhibition. The results also suggest that disrupting neural transcription factors essential for neurogenesis can prevent malignancy. We propose to analyze mechanisms of cellular transformation and the evolution of the cancer phenotype in planarians.