Additional file 1 of Monitoring in vivo behavior of size-dependent fluorescent particles as a model fine dust
Creators: name: Son, Taewoong
name: Cho, Youn-Joo
name: Lee, Hyunseung
name: Cho, Mi Young
name: Goh, Byeongwoo
name: Kim, Hyun Min
name: Hoa, Phan Thi Ngoc
name: Cho, Sun-Hee
name: Park, Young-Jun
name: Park, Hye Sun
name: Hong, Kwan Soo
Publisher: name: figshare
Year: 2022
Subjects: subject: Molecular Biology
subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: FOS: Biological sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Cancer

description: Additional file 1: Fig. S1. (a) Cy7 encapsulation efficiency, loading amount of Cy7 in CSPM, and fluorescence intensity of CSPM with various amounts of Cy7 used for particle formulation. (b) Fluorescence intensity of CSPM with various amounts of Cy7 used for particle formulation. (c) Fluorescence intensity of 0.2 mg CSPM with various loading amounts of Cy7 (nmol). (d) FE-SEM images of the synthesized CSPM with various amounts of Cy7 used for particle formulation. Fluorescence signals were recorded using a microplate reader (VICTOR X2 Multilabel PerkinElmer, MA, USA; λex/λem = 660/750 nm). Fig. S2. The morphological stability of CSPMs. Representative FE-SEM images of (a) CSPM0.1 and (b) CSPM2.0 before (0 day) and after 11 and 21 days incubation in saline and 10% FBS contained media at 37 °C. Fig. S3. Fluorescence intensity changes of (a) CSPM0.1 (2.4 mg/ml) and (b) Cy7 dye (0.625 μg/ml) after incubating with various reactive oxygen species (2 μM H2O2, 10 mM GSH, 10 mM H2S) at different time points. Fluorescence signals were recorded using a microplate reader (VICTOR X2 Multilabel PerkinElmer, MA, USA; λex/λem = 660/750 nm). Fig. S4. Synthesis and characterization of CSPM2.0. (a) A representative FE-SEM image (scale bar = 1 μm). The inset shows HR-TEM image (scale bar = 2 μm). (b) Size distribution analysis of the synthesized CSPM2.0. (c) Surface zeta potentials of silica particulate matter (SPM2.0) without dye-doping and CSPM2.0. (d) Fluorescence spectra (excitation and emission scan modes) of CSPM2.0. Fig. S5. (a) FT-IR spectra of SPM0.1, CSPM0.1, SPM2.0, and CSPM2.0. (b) Magnified spectra of SPM0.1 and CSPM0.1. The bands at 795 and 445 cm-1 are assigned to Si–O–Si stretching and Si–O–Si bending, respectively. The absorption band at 1,053 cm-1 is assigned to the siloxane vibrations of (SiO)n groups. The peaks at 3,268 and 1,634 cm-1 are attributed to O–H stretching band of the surface of silanol groups and residual water molecules. After doping of Cy7-APTES in silica particles, the new bands (1,524 cm−1 and 3,615 cm−1) are assigned to N–H bending and N-H stretching vibration of amine groups, respectively [1]. Fig. S6. The quantitative elemental analysis using ICP-OES for Si from the excised organs (n &gt; 5) after injection of CSPM0.1 into the mice. The data shown are averages of &gt;3 independent experiments. Fig. S7. Cell viability of RAW264.7 (mouse macrophage cell) and MRC-5 (human fibroblast cell) treated with CSPM0.1 and CSPM2.0 as a function of CSPM concentration. The cells were treated with CSPMs for 24 and 48 h and added the CellTiter 96 solution (Promega, WI, USA) and incubated for 1 h. Absorbance was measured at 490 nm using microplate absorbance spectrophotometer (BioRAD, CA, USA). The data shown are averages of three independent experiments. Table S1. Fluorochrome-conjugated antibodies and flow cytometer setup.