Additional file 1: of A multiplatform metabolomic approach to characterize fecal signatures of negative postnatal events in chicks: a pilot study
Creators: name: Beauclercq, Stéphane
name: Lefèvre, Antoine
name: Montigny, Frédéric
name: Collin, Anne
name: Tesseraud, Sophie
name: Leterrier, Christine
name: Emond, Patrick
name: Guilloteau, Laurence
Publisher: name: figshare
Year: 2019
Subjects: subject: Biochemistry
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Genetics
subject: Neuroscience
subject: Physiology
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: FOS: Chemical sciences
subject: Sociology
subject: FOS: Sociology
subject: FOS: Sociology
subject: Immunology
subject: FOS: Clinical medicine
subject: FOS: Clinical medicine
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics
subject: FOS: Mathematics
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: FOS: Health sciences

description: Table S1. Fecal VOCs annotated after headspace solid-phase microextraction (capture 1 h at 60 °C, 85 μm polyacrylate fiber) coupled with GC-MS. This volatilome was acquired on a pool of Hubbard control group chick feces. Figure S1. Score plots following principal component analysis for LC-HRMS (a, b, c) and GC-MS (d, e, f) fecal metabolomics. The first components (t [1]) explained 32% and 37% of the fecal metabolome variability while the second one (t [2]) explained 10 and 15% of variability in LC-HRMS and GC-MS, respectively. In the “a” and “d” score plots, Hubbard (H) individuals are represented in turquoise and Ross (R) individuals in purple. The sex of the chicks: male (M, blue) and female (F, red) are represented in the “b” and “e” score plots. The individuals from the delayed group (D, orange triangle) and control group (C, light blue circle) are represented in the “c” and “f” plots. Figure S2. OPLS-DA models adjusted to Hubbard (a) and Ross (b) chick fecal metabolomes at 12 d of age analyzed by LC-HRMS. The individuals from the delayed group (D, orange triangle) and control group (C, light blue circle) are represented in the score plots at the top of the figure. The fecal metabolites included in the Hubbard and Ross OPLS-DA models are tabulated with their variable importance in projection (VIP) and their contribution in the models. A negative contribution score indicates a contribution of the variable to the delayed group while a positive score is indicative of a contribution to the control group. The performance characteristics of each OPLS-DA model are under the metabolites tables (p = predictive component, o = orthogonal component). Figure S3. OPLS-DA models adjusted on Hubbard (a) and Ross (b) chick fecal metabolomes at 12 d of age analyzed by GC-MS. The individuals from the delayed group (D, orange triangle) and control group (C, light blue circle) are represented in the score plots at the top of the figure. The fecal metabolites included in the Hubbard and Ross OPLS-DA models are tabulated with their variable importance in projection (VIP) and their contribution in the models. A negative contribution score indicates a contribution of the variable to the delayed group while a positive score is indicative of a contribution to the control group. The performance characteristics of each OPLS-DA model are under the metabolites tables (p = predictive component, o = orthogonal component). (PDF 1250 kb)