Polymethyldopamin@Fe<sub>3</sub>O<sub>4</sub> for Magnetic Solid Phase Extraction of Polycyclic Aromatic Hydrocarbons Combined with DES as Desorption Solvent and High-Performance Liquid Chromatography-Ultraviolet Detection
Creators: name: Ghani, Milad
name: Maleki, Behrooz
name: Jafari, Zahra
name: Veisi, Hojat
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Biochemistry
subject: Space Science
subject: Cell Biology
subject: Molecular Biology
subject: Biotechnology
subject: Environmental Sciences not elsewhere classified
subject: Chemical Sciences not elsewhere classified
subject: Sociology
subject: FOS: Sociology
subject: Biological Sciences not elsewhere classified

description: Herein, Polymethyldopamin@Fe<sub>3</sub>O<sub>4</sub> (PMDP@Fe<sub>3</sub>O<sub>4</sub>) has been synthesized using a very simple, easy, cost-effective, efficient, and fast method. First, magnetic nanoparticles (Fe<sub>3</sub>O<sub>4</sub>) were synthesized. It was followed by accommodating polymethyldopamin (PMDP) on the prepared Fe<sub>3</sub>O<sub>4</sub>. The prepared PMDP@Fe<sub>3</sub>O<sub>4</sub> was utilized as a sorbent in the Magnetic Solid Phase Extraction (MSPE) of six selected Polycyclic Aromatic Hydrocarbons (PAHs) including anthracene, fluoranthene, fluorene, naphthalene, phenanthrene, and pyrene. The extracted model analytes were desorbed by a Deep Eutectic Solvent (DES) and were analyzed by High-Performance Liquid Chromatography-Ultraviolet detection (HPLC-UV). The method is validated according to ICH guidelines with respect to precision, accuracy, linearity, specificity, robustness, and limits of detection and quantification. Under the optimized condition, the linearity of the method was in the range of 0.1–500 µg L<sup>−1</sup> for the selected analytes. The calibration curves showed a determination of coefficient (<i>r</i><sup>2</sup>) higher than 0.9986. The limits of detection (LODs) and limits of quantification (LOQs) were calculated to be between 0.03–0.14 and 0.09–0.46 µg L<sup>−1</sup>, respectively. The inter-day and intra-day relative standard deviations (RSDs%) were determined to be in the range of 1.7–4.2% in three concentrations. Furthermore, the enrichment factors were found to be 76, 61, 65, 78, 69, and 67 for anthracene, fluoranthene, fluorene, naphthalene, phenanthrene, and pyrene, respectively. Moreover, the calculated absolute recoveries were between 61 and 78%. The results of the real sample analysis and the obtained recoveries indicated that the method was useful and applicable in complicated real samples. PMDP@Fe<sub>3</sub>O<sub>4</sub> nanoparticles were used for the extraction and determination of PAHs.DES was synthesized and used as a desorption solvent.The prepared sorbent showed high extraction performance, high stability, satisfactory reproducibility, and easy preparation.PMDP@Fe<sub>3</sub>O<sub>4</sub> nanoparticles revealed satisfactory extraction recoveries for the MSPE of the PAHs.The method is effective, sensitive, cost-effective, and has wide LDRs and low LODs. PMDP@Fe<sub>3</sub>O<sub>4</sub> nanoparticles were used for the extraction and determination of PAHs. DES was synthesized and used as a desorption solvent. The prepared sorbent showed high extraction performance, high stability, satisfactory reproducibility, and easy preparation. PMDP@Fe<sub>3</sub>O<sub>4</sub> nanoparticles revealed satisfactory extraction recoveries for the MSPE of the PAHs. The method is effective, sensitive, cost-effective, and has wide LDRs and low LODs.