Sodium butyrate alleviates heat stress-induced damage to morphology, anti-oxidant status, inflammatory response and barrier integrity in jejunum of broilers
Creators: name: Ruixia, Lan
name: Wu, Fan
name: Wang, Yucheng
name: Yin, Fuquan
Publisher: name: Taylor & Francis
Year: 2024
Subjects: subject: Space Science
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Molecular Biology
subject: Physiology
subject: Environmental Sciences not elsewhere classified
subject: Chemical Sciences not elsewhere classified
subject: Science Policy
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Mental Health

description: To evaluate the protective effects of sodium butyrate (SB) on intestinal morphology, anti-oxidant status, inflammatory response and barrier integrity in male broilers under heat stress. A total of 180 21-d-old male Arbour Acres broilers with similar body weight were used for this 21-d experiment. Broilers were randomly allocated to three groups: CON group, basal diet and raised under 24 °C; HS group, basal diet and raised under heat stress condition (34 °C from 10:00 to 18:00 and 24 °C for the rest time); HS-SB group, basal diet with 1200 mg/kg SB and raised under heat stress condition. Compared to the HS group, SB supplementation increased average daily gain (ADG), villus height: crypt depth ratio (VH: CD), VH, glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) activity, and interleukin-10 (IL-10) level, decreased feed conversion ratio (FCR), CD, reactive oxygen species (ROS), IL-1β and tumour necrosis factor-alpha (TNF-α) level, up-regulated the mRNA expression of nuclear factor erythroid 2-related factor 2, haem oxygenase 1, GSH 1 <i>(GPX1)</i>, <i>SOD</i> and <i>CAT</i>, down-regulated the mRNA expression of toll-like receptor 4 (TLR4), nuclear transcription factors, <i>IL-1β</i>, and <i>TNF-α</i> in jejunum of heat-stressed broilers. Furthermore, SB supplementation up-regulated the mRNA expression of <i>occluding</i>, <i>claudin-1</i> and <i>zona occludens-1</i> (<i>ZO-1</i>) in jejunum of heat-stressed broilers, accompanied with decreasing serum endotoxin and D-lactic acid concentration, and diamine oxidase (DAO) activity. There results suggested that SB supplementation could alleviate heat stress-induced jejunal tight junction structure dysfunction by up-regulated the expression of <i>occluding</i>, <i>claudin-1</i> and <i>ZO-1</i>, presumably through alleviating oxidative stress and inflammatory response.HIGHLIGHTSSodium butyrate alleviated heat stress-induced jejunal morphology damage.Sodium butyrate alleviated heat stress-induced jejunal oxidative damage.Sodium butyrate alleviated heat stress-induced jejunal inflammatory response.Sodium butyrate alleviated heat stress-induced tight junction structure dysfunction. Sodium butyrate alleviated heat stress-induced jejunal morphology damage. Sodium butyrate alleviated heat stress-induced jejunal oxidative damage. Sodium butyrate alleviated heat stress-induced jejunal inflammatory response. Sodium butyrate alleviated heat stress-induced tight junction structure dysfunction.