Additional file 1 of Palmitoyl transferases act as novel drug targets for pancreatic cancer
Creators: name: Lin, Zhiqing
name: Lv, Ziru
name: Liu, Xin
name: Huang, Keke
Publisher: name: figshare
Year: 2023
Subjects: subject: Biochemistry
subject: Space Science
subject: Genetics
subject: FOS: Biological sciences
subject: Pharmacology
subject: Chemical Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Cancer

description: Additional file 1: Fig. S1. Representative immunohistochemical images of ZDHHC family members in human pancreatic cancer tissue from the HPA database. Fig. S2. Relationship between expression levels of ZDHHC3/4/7/9/15/17/20/22 and pathological stages of PAAD patients. A-H The association between mRNA expression of ZDHHCs and patients’ individual cancer stages of PAAD from the GEPIA database; p &lt; 0.05 was considered statistically significant. Fig. S3. Relationship between ZDHHCs gene expression and pathological stages of PAAD patients. A-O The association between mRNA expression of ZDHHCs and patients’ individual cancer stages of PAAD from the GEPIA database. Fig. S4. Univariate and multivariate cox regression were used to test prognostic significance of ZDHHCs in TCGA-PAAD datasets. A-B The effect of ZDHHCs expression on overall survival (OS) (A) and relapse-free survival (RFS) (B) from the K-M plotter database. Fig. S5. Associations between ZDHHC3 expression and overall survival time in pan-cancers based on TCGA database. Fig. S6. Associations between ZDHHC3 expression and disease-specific survival in pan-cancers based on TCGA database. Fig. S7. Correlation between TMB or MSI and ZDHHC3 expression in pan-cancers. A A stick chart showing the correlation between the ZDHHC3 and TMB in different tumor types. The red curve represents the Spearman correlation coefficient, and the black value represents the range. B A stick chart showing the correlation between the ZDHHC3 and MSI in different tumor types. Fig. S8. Differentially expressed chemokines and paired receptors between high- and low-ZDHHC3 PAAD patients. A Boxplot showing the chemokines and paired receptors with significant expression differences between high- and low-ZDHHC3 groups in TCGA-PAAD cohort. B Boxplot showing the chemokines and paired receptors with no significant expression differences between high- and low-ZDHHC3 groups in TCGA-PAAD cohort. The R software ggpubr package was used to draw boxplots and perform Student’s t-test statistical analyses. Results shown are presented as mean ± SD. (ns, not significant, *p &lt; 0.05, **p &lt; 0.01, and ***p &lt; 0.001). Fig. S9. Mutation landscape between high- and low-ZDHHC3 in PAAD. A mutation landscape shows the differences in gene mutation frequencies in each set of PAAD samples using chi-square tests to classify the top 15 genes with the highest mutation frequencies according to the expression of ZDHHC3. Fig. S10. KEGG enrichment and pathway correlation analysis for ZDHHC3 in PAAD. A Top 20 KEGG enrichment pathways in PAAD. B The abscissa represents the distribution of the ZDHHC3 expression, and the ordinate represents the distribution of the pathway score. The density curve on the right represents the trend in distribution of pathway immune score, the upper density curve represents the trend in distribution of the ZDHHC3 expression. The value on the top represents the correlation p value, correlation coefficient and correlation calculation method. Fig. S11. Concentrations of CCL4, CCL5, CXCL9, and CXCL10 in shControl and shZDHHC3 Panc 02 tumor tissues on day 23 after tumor inoculation measured by ELISA. Results shown are presented as mean ± SD. (ns, not significant, *p &lt; 0.05, and **p &lt; 0.01). Fig. S12. ZDHHC3 knockdown inhibits PD-L1 expression at protein level in pancreatic cancer cells. (A-D) PANC-1 cells were transfected with siRNA. After 72 h, cells were harvested for (A) western blotting analysis, (B) RT-qPCR analysis, (C) flow cytometry assay and (D) corresponding quantification. (E) Representative immunofluorescence images of ZDHHC3 (green) and PD-L1 (red)staining of PANC-1 cells (blue, DAPI-labelled cell nuclei) and (F) corresponding quantification (Scale bars, 20 µm). (G) Reciprocal co-IP assay was performed to examine the interaction between endogenously expressed ZDHHC3 and PD-L1 in PANC-1 cells. Results shown are presented as mean ± SD. (ns, not significant and ***p &lt; 0.001). Fig. S13. Relative expression of CCL4, CCL5, CXCL9, and CXCL10 in CTRL and 2-BP treated Panc 02 cells in vitro. Concentrations of CCL4, CCL5, CXCL9, and CXCL10 in CTRL and 2-BP treated Panc 02 cells for 24 h were measured by ELISA. Results shown are presented as mean ± SD. (ns, not significant and ***p &lt; 0.001).