title: ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð»ÑÐ¼Ð¸Ð½Ð¸ÑÑÐµÐ½ÑÐ¸Ð¸ ÑÐ°ÑÑÐ²Ð¾ÑÐ° Ð°Ð»ÐºÐ¾Ð³Ð¾Ð»Ñ Ð´ÐµÐ³Ð¸Ð´ÑÐ¾Ð³ÐµÐ½Ð°Ð·Ñ Ð¿ÑÐ¸ Ð´Ð²ÑÑ ÑÐ¾ÑÐ¾Ð½Ð½Ð¾Ð¼ Ð²Ð¾Ð·Ð±ÑÐ¶Ð´ÐµÐ½Ð¸Ð¸ Ð½Ð° Ð´Ð»Ð¸Ð½Ð°Ñ Ð²Ð¾Ð»Ð½ 720 Ð¸ 840 Ð½Ð¼
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Study of luminescence of alcohol dehydrogenase solution under two-photon excitation at 720 nm and 840 nm
Creators: name: ÐÑÑÐºÐ¾Ð½Ð¾Ð², ÐÐµÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ñ
NADH
Ð°Ð»ÐºÐ¾Ð³Ð¾Ð»Ñ Ð´ÐµÐ³Ð¸Ð´ÑÐ¾Ð³ÐµÐ½Ð°Ð·Ð°
FAD
Ð²ÑÐµÐ¼ÐµÐ½Ð° Ð·Ð°ÑÑÑ Ð°Ð½Ð¸Ñ
ÑÐ¿ÐµÐºÑÑÑ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸
Ð´Ð²ÑÑ ÑÐ¾ÑÐ¾Ð½Ð½Ð¾Ðµ Ð²Ð¾Ð·Ð±ÑÐ¶Ð´ÐµÐ½Ð¸Ðµ
fluorescence
alcohol dehydrogenase
decay
times
fluorescence spectra

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸ ÑÐ°ÑÑÐ²Ð¾ÑÐ° Ð°Ð»ÐºÐ¾Ð³Ð¾Ð»ÑÐ´ÐµÐ³Ð¸Ð´ÑÐ¾Ð³ÐµÐ½Ð°Ð·Ñ (ADH) Ð¿ÑÐ¸ Ð´Ð²ÑÑÑÐ¾ÑÐ¾Ð½Ð½Ð¾Ð¼ Ð²Ð¾Ð·Ð±ÑÐ¶Ð´ÐµÐ½Ð¸Ð¸ Ð¿Ð¸ÐºÐ¾ÑÐµÐºÑÐ½Ð´Ð½ÑÐ¼Ð¸ Ð»Ð°Ð·ÐµÑÐ½ÑÐ¼Ð¸ Ð¸Ð¼Ð¿ÑÐ»ÑÑÐ°Ð¼Ð¸ Ñ Ð´Ð»Ð¸Ð½Ð½Ð¾Ð¹ Ð²Ð¾Ð»Ð½Ñ 720 Ð¸ 840 Ð½Ð¼. Ð¦ÐµÐ»ÑÑ ÑÑÐ¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÐµÑÑÑ Ð°Ð½Ð°Ð»Ð¸Ð· ÑÐ¿ÐµÐºÑÑÐ¾Ð² ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸ Ð¸ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÐ¾Ð² Ð·Ð°ÑÑÑÐ°Ð½Ð¸Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÐºÐ¾ÑÐµÑÐ¼ÐµÐ½ÑÐ° NADH Ð² ÑÐ°ÑÑÐ²Ð¾ÑÐµ Ñ ÑÐµÑÐ¼ÐµÐ½ÑÐ¾Ð¼ Ð°Ð»ÐºÐ¾Ð³Ð¾Ð»ÑÐ´ÐµÐ³Ð¸Ð´ÑÐ¾Ð³ÐµÐ½Ð°Ð·Ð¾Ð¹. ÐÐ»Ñ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ ÑÐ¸Ð³Ð½Ð°Ð»Ð¾Ð² Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð°ÑÑ ÑÐ¸ÑÑÐµÐ¼Ð° ÑÑÐµÑÐ° ÐµÐ´Ð¸Ð½Ð¸ÑÐ½ÑÑ ÑÐ¾ÑÐ¾Ð½Ð¾Ð² Ñ Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð¹ ÐºÐ¾ÑÑÐµÐ»ÑÑÐ¸ÐµÐ¹ (TCSPC). Ð ÑÐ°Ð¼ÐºÐ°Ñ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ñ ÑÐ¿ÐµÐºÑÑÑ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸ ÑÐ°ÑÑÐ²Ð¾ÑÐ° ADH Ð¿ÑÐ¸ Ð´Ð»Ð¸Ð½Ð°Ñ Ð²Ð¾Ð»Ð½ Ð²Ð¾Ð·Ð±ÑÐ¶Ð´ÐµÐ½Ð¸Ñ 720 Ð¸ 840 Ð½Ð¼. Ð¢Ð°ÐºÐ¶Ðµ Ð±ÑÐ»Ð¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ñ Ð¸Ð·Ð¼ÐµÑÐµÐ½Ð¸Ñ ÑÐ¸Ð³Ð½Ð°Ð»Ð¾Ð² Ð·Ð°ÑÑÑÐ°Ð½Ð¸Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸ ÑÐ°ÑÑÐ²Ð¾ÑÐ° ADH Ð¿ÑÐ¸ Ð´Ð»Ð¸Ð½Ð°Ñ Ð²Ð¾Ð»Ð½ Ð²Ð¾Ð·Ð±ÑÐ¶Ð´ÐµÐ½Ð¸Ñ 720 Ð¸ 840 Ð½Ð¼, Ð² Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½ÑÑ ÑÐ¿ÐµÐºÑÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð¸Ð°Ð¿Ð°Ð·Ð¾Ð½Ð°Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸ 425-445 Ð½Ð¼ Ð¸ 460-480 Ð½Ð¼. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐ¸Ð³Ð½Ð°Ð»Ñ Ð·Ð°ÑÑÑÐ°Ð½Ð¸Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸ Ð±ÑÐ»Ð¸ Ð¿ÑÐ¾Ð°Ð½Ð°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°Ð½Ñ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÑÑÐ½ÐºÑÐ¸Ð¹ ÑÑÐ¼Ð¼Ñ Ð´Ð²ÑÑ Ð¸ ÑÑÐµÑ ÑÐºÑÐ¿Ð¾Ð½ÐµÐ½Ñ, Ð³Ð´Ðµ Ð²Ð°Ð¶Ð½ÑÐ¼Ð¸ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÐ°Ð¼Ð¸ ÑÐ²Ð»ÑÐ»Ð¸ÑÑ Ð²ÑÐµÐ¼ÐµÐ½Ð° Ð¶Ð¸Ð·Ð½Ð¸ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸ Ð¸ Ð²ÐµÑÐ¾Ð²ÑÐµ ÐºÐ¾ÑÑÑÐ¸ÑÐ¸ÐµÐ½ÑÑ. Ð¢Ð°ÐºÐ¶Ðµ Ð±ÑÐ»Ð¾ Ð¿ÑÐ¾Ð¸Ð·Ð²ÐµÐ´ÐµÐ½Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ðµ Ð¼Ð¾Ð´ÐµÐ»ÐµÐ¹, Ð´Ð»Ñ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾ÑÑÐ¸ Ð²ÑÐ±Ð¾ÑÐ° Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼Ð¾Ð³Ð¾ Ð¸ Ð´Ð¾ÑÑÐ°ÑÐ¾ÑÐ½Ð¾Ð³Ð¾ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð° ÑÐºÑÐ¿Ð¾Ð½ÐµÐ½Ñ Ð´Ð»Ñ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð´Ð°Ð½Ð½ÑÑ. ÐÐ° Ð¾ÑÐ½Ð¾Ð²Ðµ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð±ÑÐ»Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ñ Ð²ÑÐ²Ð¾Ð´Ñ Ð¾ ÑÐ¾ÑÑÐ°Ð²Ðµ ÑÐ°ÑÑÐ²Ð¾ÑÐ°, Ð² ÑÐ°ÑÑÐ½Ð¾ÑÑÐ¸, Ð±ÑÐ»Ð¾ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¾ Ð½Ð°Ð»Ð¸ÑÐ¸Ðµ Ð½ÐµÑÐºÐ¾Ð»ÑÐºÐ¸Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐ¸ÑÑÑÑÐ¸Ñ Ð¼Ð¾Ð»ÐµÐºÑÐ», ÑÐ°ÐºÐ¸Ñ ÐºÐ°Ðº FAD Ð¸ NADH Ð² ÑÐ²Ð¾Ð±Ð¾Ð´Ð½Ð¾Ð¹ Ð¸ ÑÐ²ÑÐ·Ð°Ð½Ð½Ð¾Ð¹ Ñ Ð±ÐµÐ»ÐºÐ¾Ð¼ ÑÐ¾ÑÐ¼Ð°Ñ.
description: The given work is devoted to the study of fluorescence of an alcohol dehydrogenase solution under two-photon excitation at 720 nm and 840 nm by picosecond laser pulses. The purpose of the work is to determine the fluorescence decay parameters of the biological coenzyme NADH in the solution with alcohol dehydrogenase. To obtain the experimental signals a Time-Correlated Single Photon Counting (TCSPC) system was used. he fluorescence spectra of the alcohol dehydrogenase solution were obtained within the work. Moreover, the signals of fluorescence decay of the ADH solution were measured at excitation wavelengths 720 nm and 840 nm, in chosen spectral ranges of fluorescence 425-445 nm and 460- 480 nm at excitation wavelength 720 nm and 840 nm. The measured fluorescence signals were analyzed using two-exponential and three-exponential functions, those include decay times and pre-exponential factors itself. Also, the models were compared to each other, in order to choose the sufficient number of exponents for the analysis. Based on the analysis of the obtained data, conclusions were drawn about the composition of the solution. Several fluorescent molecules were found such as FAD and NADH in both the free and enzyme-bound forms.