title: ÐÐ¾Ð»ÑÑÐµÐ½Ð¸Ðµ Ñ Ð¾Ð»Ð¾Ð´Ð¾Ð°Ð´Ð°Ð¿ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ ÑÑÐ°Ð¼Ð¼Ð° Ð²Ð¸ÑÑÑÐ° Ð³ÑÐ¸Ð¿Ð¿Ð° Ð Ñ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¼ Ð³ÐµÐ½Ð¾Ð¼ NS Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð³ÐµÐ½Ð½Ð¾Ð¹ Ð¸Ð½Ð¶ÐµÐ½ÐµÑÐ¸Ð¸ Ð¸ Ð¾ÑÐµÐ½ÐºÐ° ÐµÐ³Ð¾ ÑÐ²Ð¾Ð¹ÑÑÐ²
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Derivation of cold-adapted influenza A strain with modified NS gene by generic engineering and studying of its properties
Creators: name: Ð§Ð¸ÑÑÑÐºÐ¾Ð²Ð°, ÐÐ½Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: Ð²Ð¸ÑÑÑ Ð³ÑÐ¸Ð¿Ð¿Ð° Ð
Ð¶Ð¸Ð²Ð°Ñ Ð³ÑÐ¸Ð¿Ð¿Ð¾Ð·Ð½Ð°Ñ Ð²Ð°ÐºÑÐ¸Ð½Ð°
Ð²ÐµÐºÑÐ¾ÑÐ½Ð°Ñ Ð²Ð°ÐºÑÐ¸Ð½Ð°
Ð³ÐµÐ½Ð½Ð°Ñ Ð¸Ð½Ð¶ÐµÐ½ÐµÑÐ¸Ñ
Ð²Ð¸ÑÑÑÐ¾Ð»Ð¾Ð³Ð¸Ñ
influenza
live attenuated influenza vaccine (LAIV)
virology
vector vaccine
molecular biology

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° ÑÐ¾Ð·Ð´Ð°Ð½Ð¸Ñ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ ÑÐ¾Ð»Ð¾Ð´Ð¾Ð°Ð´Ð°Ð¿ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð² Ð²Ð¸ÑÑÑÐ¾Ð² Ð³ÑÐ¸Ð¿Ð¿Ð° A Ñ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¼ Ð³ÐµÐ½Ð¾Ð¼ NS Ð´Ð»Ñ Ð¸Ð½Ð´ÑÐºÑÐ¸Ð¸ ÑÐ°ÑÑÐ¸ÑÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¸Ð¼Ð¼ÑÐ½Ð½Ð¾Ð³Ð¾ Ð¾ÑÐ²ÐµÑÐ° Ð½Ð° Ð²Ð¸ÑÑÑÑ Ð³ÑÐ¸Ð¿Ð¿Ð°. ÐÐ¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ñ Ð·Ð°ÐºÐ»ÑÑÐ°ÐµÑÑÑ Ð²Ð¾ Ð²ÑÑÐ°Ð²ÐºÐµ Ð¸Ð¼Ð¼ÑÐ½Ð¾Ð³ÐµÐ½Ð½ÑÑ, ÐºÐ¾Ð½ÑÐµÑÐ²Ð°ÑÐ¸Ð²Ð½ÑÑ ÑÑÐµÐ´Ð¸ Ð²Ð¸ÑÑÑÐ¾Ð² Ð³ÑÐ¸Ð¿Ð¿Ð° ÑÐ°Ð·Ð½ÑÑ Ð¿Ð¾Ð´ÑÐ¸Ð¿Ð¾Ð² ÑÐ¿Ð¸ÑÐ¾Ð¿Ð¾Ð² Ð² Ð³ÐµÐ½, ÐºÐ¾Ð´Ð¸ÑÑÑÑÐ¸Ð¹ NS1. Ð¦ÐµÐ»ÑÑ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÐ»Ð¾ÑÑ Ð¼ÐµÑÐ¾Ð´Ð°Ð¼Ð¸ Ð³ÐµÐ½Ð½Ð¾Ð¹ Ð¸Ð½Ð¶ÐµÐ½ÐµÑÐ¸Ð¸ Ð¿Ð¾Ð»ÑÑÐ¸ÑÑ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¹ ÑÐµÐ³Ð¼ÐµÐ½Ñ Ð³ÐµÐ½Ð¾Ð¼Ð° Ð²Ð¸ÑÑÑÐ° Ð¸ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð¾Ð±ÑÐ°ÑÐ½Ð¾Ð¹ Ð³ÐµÐ½ÐµÑÐ¸ÐºÐ¸ Ð¿Ð¾Ð»ÑÑÐ¸ÑÑ 3 ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð²Ð°ÐºÑÐ¸Ð½Ð½ÑÑ ÑÑÐ°Ð¼Ð¼Ð° Ñ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¼ Ð³ÐµÐ½Ð¾Ð¼ NS, Ð¿Ð¾ÑÐ»Ðµ ÑÐµÐ³Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÑ Ð¸Ñ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð°. ÐÐ»Ñ ÑÑÐ¾Ð³Ð¾ Ð±ÑÐ»Ð¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ Ð´Ð¾Ð½Ð¾Ñ Ð°ÑÑÐµÐ½ÑÐ°ÑÐ¸Ð¸ Ð/ÐÐµÐ½Ð¸Ð½Ð³ÑÐ°Ð´/134/17/57, ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð½ÑÐ¹ Ð² Ð¸Ð½ÑÑÐ¸ÑÑÑÐµ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð¼ÐµÐ´Ð¸ÑÐ¸Ð½Ñ, Ð¸ ÑÐ¾Ð²ÑÐµÐ¼ÐµÐ½Ð½ÑÐµ ÑÑÐ°Ð¼Ð¼Ñ Ð²Ð¸ÑÑÑÐ¾Ð² Ð³ÑÐ¸Ð¿Ð¿Ð° Ð¿Ð¾Ð´ÑÐ¸Ð¿Ð¾Ð² H1N1, H3N2, H7N9. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð±ÑÐ»Ð¾ ÑÑÐ¿ÐµÑÐ½Ð¾ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¾ 3 Ð²Ð°ÐºÑÐ¸Ð½Ð½ÑÑ ÑÑÐ°Ð¼Ð¼Ð°, Ð¾Ð±Ð»Ð°Ð´Ð°Ð²ÑÐ¸Ðµ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑÑ Ðº ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð¸ Ð¿ÑÐ¸ Ð¾Ð¿ÑÐ¸Ð¼Ð°Ð»ÑÐ½Ð¾Ð¹ ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÐµ, ÑÐ²Ð»ÑÑÑÐ¸ÐµÑÑ ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÐ¾ÑÑÐ²ÑÑÐ²Ð¸ÑÐµÐ»ÑÐ½ÑÐ¼Ð¸ Ð¸ ÑÐ°Ð·Ð¼Ð½Ð¾Ð¶Ð°ÑÑÐ¸Ð¼Ð¸ÑÑ Ð¿ÑÐ¸ ÑÐ½Ð¸Ð¶ÐµÐ½Ð½Ð¾Ð¹ ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÐµ. Ð¨ÑÐ°Ð¼Ð¼Ñ, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÐµ Ð² ÑÐ¾Ð´Ðµ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð¸Ñ ÑÐ°Ð±Ð¾ÑÑ, Ð±ÑÐ´ÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ Ð´Ð»Ñ Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐµÐ³Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ ÑÐ½Ð¸Ð²ÐµÑÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð²Ð°ÐºÑÐ¸Ð½Ñ Ð¿ÑÐ¾ÑÐ¸Ð² Ð³ÑÐ¸Ð¿Ð¿Ð°.
description: This study reports the development of cold-adapted strains of influenza A viruses with a modified NS gene for the induction of an extended immune response to influenza viruses. The modification comprises the insertion of immunogenic epitopes conserved among influenza viruses of different subtypes into the gene encoding NS1. The aim of this work was to obtain a modified segment of the virus genome using genetic engineering methods and to rescue 3 experimental vaccine strains with a modified NS gene using the reverse genetics method, and then study their properties. For this purpose, the A/Leningrad/134/17/57 master donor virus was used, and current influenza strains of H1N1, H3N2 and H7N9. As a result, 3 vaccine strains were successfully rescued. The strains replicate at an optimal temperature, are temperature-sensitive and have a cold-adapted phenotype. The strains will be further studied as a candidates for influenza prophylaxis as an experimental universal influenza vaccine.