MOESM1 of Engineering Escherichia coli FAB system using synthetic plant genes for the production of long chain fatty acids
Creators: name: Kassab, Elias
name: Fuchs, Monika
name: Haack, Martina
name: Mehlmer, Norbert
name: Brueck, Thomas
Publisher: name: figshare
Year: 2019
Subjects: subject: Biochemistry
subject: Space Science
subject: Microbiology
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Genetics
subject: Molecular Biology
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: FOS: Chemical sciences
subject: Science Policy
subject: 69999 Biological Sciences not elsewhere classified
subject: Plant Biology

description: Additional file 1: Table S1. Fatty acid distribution in μg/g DCW of wild type Escherichia coli BL21 (DE3) at different time intervals. T = 0: directly before induction with IPTG, t = 12: 12 h after induction with IPTG, t = 24: 24 h after induction with IPTG. All fatty acid values are the average of at least three biological replicates with the associated standard deviation indicated. Table S2. Fatty acid distribution in μg/g DCW of Escherichia coli BL21 (DE3) expressing pET28aKASI at different time intervals. T = 0: directly before induction with IPTG, t = 12: 12 h after induction with IPTG, t = 24: 24 h after induction with IPTG. All fatty acid values are the average of at least three biological replicates with the associated standard deviation indicated. Table S3. Fatty acid distribution in μg/g DCW of Escherichia coli BL21 (DE3) ΔfadD pET28a− versus BL21 (DE3) ΔfadD expressing pET28aKASI and pET28aKASII separately. Samples were collected 48 h after induction with IPTG. All fatty acid values are the average of at least three biological replicates with the associated standard deviation indicated. Table S4. Fatty acid distribution in μg/g DCW of Escherichia coli BL21 (DE3) ΔfadF pET28a− versus BL21 (DE3) ΔfadD expressing pET28aKASII. Samples were collected 48 h after induction with IPTG. All fatty acid values are the average of at least three biological replicates with the associated standard deviation indicated. Table S5. Fatty acid distribution in μg/mg DCW of the 1.3 L fermentation of Escherichia coli BL21 (DE3) ΔfadD expressing an empty pET28a vector, pET28aKASI and pET28aKASII respectively. Samples were collected 48 h after induction with IPTG. All fatty acid values are the average of at least three biological replicates with the associated standard deviation indicated. Table S6. Fatty acid distribution in μg/mg DCW of the 1.3 L fermentation of Escherichia coli BL21 (DE3) ΔfabF expressing pET28aKASI and pET28aKASII respectively. Samples were collected 48 h after induction with IPTG. All fatty acid values are the average of at least three biological replicates with the associated standard deviation indicated. Table S7. Growth analysis of M9 minimal media shake flask studies of wild type Escherichia coli BL21 (DE3) expressing pET28a−, pET28aKASI and pET28aKASII respectively. Absorbance values (OD600) are the average of at least three biological replicates. Figure S1. Growth curve of M9 minimal media shake flask studies of wild type Escherichia coli BL21 (DE3) expressing pET28a−, pET28aKASI and pET28aKASII respectively. Absorbance values (OD600) are the average of at least three biological replicates with the associated standard deviation indicated.