cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Investigating a potential role of Got1 in T cell exhaustion.
Creators: name: Weisshaar, Nina
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: Purpose/Objectives: During cancer and chronic infections, T cells can become exhausted and gradually lose their effector functions. Although clinical studies targeting immune checkpoint molecules (e.g. PD-1) lead to promising results, not all patients can benefit from that kind of treatment, indicating the mechanism of T cell exhaustion is much more complex and has to be understood in more detail in order to develop better therapies. Regulation of T cell exhaustion is not only mediated by immunosuppressive receptors, but also by cell intrinsic metabolic changes. The cytoplasmatic enzyme Glutamic-oxaloacetic transaminase 1 (Got1) plays an important role during amino acid metabolism and energy production in the cell. Since Got1 has been found to be upregulated during T cell exhaustion, our research aims to investigate a possible role of Got1 in this process.


Materials/Methods: To study the T cell-intrinsic role of Got1, we use transgenic mouse lines with Cre-loxP recombination. We are using CD4-Cre*Got1flox/flox mice to study Got1 deficiency in CD4+ and CD8+ T cells. Additionally, we use P14*CD4-Cre*Got1flox/flox mice, which bear a transgenic TCR and allow us to perform adoptive transfer experiments to investigate the CD8+ T cell-intrinsic function of Got1. The mice are challenged with Lymphocytic choriomeningitis virus strains Armstrong or cl-13 to induce acute or chronic viral infections, respectively.


Results: Got1-deficient T cells develop and proliferate normal in naïve mice. First preliminary results indicate that, after chronic viral infection, Got1-deficiency resulted in a reduction of EOMES/TCF7 high “stem cell-like” T cells. This population has previously been demonstrated to be needed for a sustained long-term chronic immune response [1, 2]


Conclusion: Our preliminary studies of Got1 during T cell exhaustion indicate an importance for this enzyme during the long term chronic immune response. By maintaining the pool of “stem cell-like” T cells that give rise to effector T cells, maintaining the long term chronic immune response, Got1 might positively influence T cell function during chronic stimulus. It will be interesting to see in the future, whether the deficiency of Got1 will also impair T cell function during cancer development.


References
[1] Im, S.J., et al., Defining CD8+ T cells that provide the proliferative burst after PD-1 therapy. Nature, 2016. 537(7620): p. 417-421.
[2] Utzschneider, D.T., et al., T Cell Factor 1-Expressing Memory-like CD8(+) T Cells Sustain the Immune Response to Chronic Viral Infections. Immunity, 2016. 45(2): p. 415-27.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)
Biparatopic heavy chain antibodies (bi-hcAbs): fusion of two distinct CD38-specific nanobodies into a human IgG1 bi-hcAb results in potent CDC vs. myeloma cells
Creators: name: Schütze, Kerstin
name: Hambach, Julia
name: Petry, Katharina
name: Fumey, William
name: Albrecht, Birte
name: Schriewer, Levin
name: Röckendorf, Jana
name: Menzel, Stephan
name: Haag, Friedrich
name: Stortelers, Catelijne
name: Bannas, Peter
name: Koch-Nolte, Friedrich
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: Purpose/Objectives: The purpose of this study was to generate CD38-specific heavy chain antibodies (hcAbs) with improved complement dependent cytotoxicity (CDC) against multiple myeloma cells. Daratumumab is an effective therapeutic mAb targeting CD38, an ecto-enzyme overexpressed on multiple myeloma and other hematological malignancies. Patients that become refractory to daratumumab have myeloma cells expressing increased levels of complement inactivating proteins (CD55, CD59), suggesting that CDC is a mechanism of action of this mAb.


Materials/Methods: We fused the soluble variable domain (VHH or nanobody) of CD38-specific llama heavy chain antibodies (hcAbs) to the hinge-CH2-CH3 domains of human IgG1 (Fig. 1). In order to compare the CDC potency of daratumumab and nanobody-based hcAbs, we incubated CD38-expressing LP-1 myeloma and CA-46 Burkitt lymphoma cells with individual Abs or combinations of Abs in the presence of human serum as a source of complement and monitored cell death by flow cytometry.


Results: Monospecific and bispecific hcAbs were produced at high yield as highly soluble proteins by transiently-transfected HEK-cells. While daratumumab showed a moderate potency to induce CDC toward LP-1 or CA-46 cells, individual hcAbs showed only little if any CDC potency. In contrast, combinations of two hcAbs recognizing distinct epitopes of CD38 potently induced CDC. Similarly, combining daratumumab with a hcAb recognizing a non-overlapping epitope of CD38 markedly increased its CDC potency. Remarkably, bispecific hcAbs containing nanobodies that recognize two non-overlapping epitopes of CD38 displayed even higher CDC inducing potency than daratumumab.


Conclusion: Our results illustrate the therapeutic potential of chimeric nanobody-based human IgG1 heavy chain antibodies. The biparatopic CD38-specific hcAbs may represent alternatives to daratumumab for the treatment of hematological malignancies.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)
Scottianella xingjiangensis Emeljanov 2019, comb. nov.
Creators: name: Emeljanov, Alexandr F.
Publisher: name: Zenodo
Year: 2019
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hemiptera
Delphacidae
Scottianella
Scottianella xingjiangensis

description: Scottianella xingjiangensis (Chen, 2002), comb. nov.
= Elachodelphax xingjiangensis Chen, 2002: 34 –36.
Note. Perhaps Elachodelphax xingjiangensis Chen is a junior synonym of Scottianella dalei (Scott, 1870), however, I do not have enough material from the type localities of the species and from the territories between the type localities to make the final decision.
description: Published as part of Emeljanov, Alexandr F., 2019, New synonyms and new combinations for some Delphacidae (Hemiptera, Auchenorrhyncha) of Eastern and Central Palaearctic Region, pp. 396-400 in Zootaxa 4559 (2) on page 399, DOI: 10.11646/zootaxa.4559.2.13, http://zenodo.org/record/2626956
Activation of MDA5 improves anti-tumor therapy and reduces suppressive capacity of myeloid-derived suppressor cells
Creators: name: Metzger, Philipp
name: Kirchleitner, Sabrina V.
name: Kluge, Michael
name: König, Lars M.
name: Endres, Stefan
name: Friedel, Caroline C.
name: Schnurr, Max
name: Duewell, Peter
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: Objective: Pancreatic cancer is infiltrated by stromal cell and suppressive immune cells, including myeloid-derived suppressor cells (MDSC). MDSC are key determinants for maintaining the immunosuppressive tumor microenvironment. Both, monocytic MDSC (M-MDSC) and polymorphonuclear MDSC (PMN-MDSC), are highly T cell suppressive. Stimulation of RIG-I-like helicases (RLH), such as melanoma differentiation antigen 5 (MDA5), induces a type I interferon (IFN)-driven immune response and induces an immunogenic form of tumor cell death in pancreatic cancer. The aim of the study is to assess the effects of RLH treatment, using the synthetic MDA5 ligand polyinosinic:polycytidylic acid (poly(I:C)), on the MDSC phenotype and function.


Methods: C57BL/6 and IFNAR-/- mice were orthotopically injected with KPC-derived T110299 pancreatic cancer cells and treated i.v. with poly(I:C), complexed with PEI. After 21 days, mice were sacrificed and immune cells from tumors and spleens were analyzed by flow cytometry. PMN-MDSC (CD11b+ Ly6G+ Ly6Cint) and M-MDSC (CD11b+ Ly6G- Ly6C+) as well as bulk tumor biopsies were isolated for RNA-sequencing as well as for functional assays. RNAseq data were subjected to KEGG pathway analysis, Gene Set Enrichment Analysis.


Results: Survival of poly(I:C)-PEI-treated mice with orthotropic pancreatic tumors was significantly prolonged, and a reduction of tumor size was observed after only two injections of poly(I:C)-PEI. The suppressive capacity of both, PMN- and M-MDSC populations was significantly reduced after poly(I:C)-PEI treatment. KEGG pathway analysis of RNA sequencing data revealed an enrichment of type I IFN signaling, virus-associated as well as antigen processing- and presentation-associated gene signature upon poly(I:C)-PEI treatment. In addition, flow cytometry confirmed the upregulation of MHC-I and CD86 surface molecules in MDSC-subpopulations. In IFNAR-/- mice, expression levels were
unaltered pointing towards a type I IFN-mediated effect. CIBERSORT analysis of bulk tumor tissue pointed towards a reduction of the M2 phenotype, which was paralleled by increased expression levels of M1-related genes, after therapy.


Conclusion: The survival benefit of RLH ligand-treated mice with pancreatic cancer correlated with the reduction in tumor size, reduced T cell suppressive capacity of MDSC and functional reprogramming towards a M1/G1 phenotype. Type I IFN signaling induced by RLHbased immunotherapy has the potential to revert tumor induced immune suppression.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)
Additional file 1 of Patient experiences of buprenorphine dispensing from a mobile medical unit
Creators: name: Messmer, Sarah E.
name: Elmes, Abigail T.
name: Infante, Alexander F.
name: Patterson, Anna
name: Smith, Mackenzie
name: Murphy, Albert Leon
name: Jimenez, Antonio D.
name: Mayer, Stockton
name: Watson, Dennis P.
name: Whitfield, Kevin
name: Fisher, Steven J.
name: Jarrett, Jennie B.
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Cell Biology
subject: Pharmacology
subject: Biotechnology
subject: Sociology
subject: FOS: Sociology
subject: Environmental Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Science Policy
subject: Mental Health
subject: Cardiology

description: Supplementary Material 1
Orde versus chaos; het Shelltijdperk binnen de stadsgeschiedenis van Willemstad/Curaçao (1920-1960)
Creators: name: Kamphuis, Mariëtte
Publisher: name: Bulletin KNOB
Year: 1993

description: [No summary available]
description: Bulletin KNOB, Bulletin KNOB 92 (1993) 1-2
Effect of both PD-1/PD-L1 and ERK inhibitor on Cisplatin-sensitive (HEY) and Cisplatin-resistant (HEY-Cis) ovarian cancer cell lines
Creators: name: Vogt, Paulina
name: Köster, Frank
name: Polack, Stephan
name: Rody, Achim
name: Bräutigam, Karen
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: Objectives: Diagnosis of ovarian cancer is associated with adverse prognosis caused by late diagnosis, a high recurrence rate and a fast emerging chemotherapy resistance. The goal was to identify a novel therapy approach for treatment of especially chemo resistant ovarian cancer by combination of a PD-1/PD-L1 and an ERK inhibitor.


Methods: After single and combined inhibitor treatment the concentration of 50 % reduction of cell viability (IC50) was determined in HEY and Hey-Cis by MTS assay. Impact on MAP kinase pathway was checked by immunoblot. QPCR experiments were performed to prove expression ratios of PD-L1, IL8, IL6, cFos, FosL und VEGFC after treatment. IL8 secretion was checked by ELISA.


Results: The PD-1/PD-L1 inhibitor provoked 50 % reduction of cell viability with concentrations of 3.92 μM in HEY and 4.03 μM in HEY-Cis. For the ERK inhibitor IC50 values were determined of 4.73 μM in HEY and of 1.96 μM in HEY-Cis. Combined treatment effected a synergistic inhibition of proliferation. ERK and S6 were dephosphorylated and the expression of c-Myc was decreased by single and especially combined inhibitor treatment. Gene expression of IL8 and IL6 was strongly increased in HEY while diminished HEY-Cis after ERK inhibitor treatment. Additionally, cFos, FosL and VEGFC expression were reduced in both cell lines. The ELISA confirmed a strong secretion of IL8 in HEY cells after ERK inhibitor treatment.


Conclusion: Both inhibitors reduced cell viability and acted synergistically when combined. Intracellular changes mainly occurred by the ERK inhibitor and provoked a stronger effect on HEY-Cis. The reduction of genes involved in inflammatory processes in HEY-Cis could suggest the use of a MAP kinase inhibitor combined with an immunotherapeutic therapy approach by existing Cisplatin resistance.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)
PhyloProfile: an interactive and dynamic visualization tool for multi- layered phylogenetic profiles
Creators: name: Ngoc-Vinh Tran
name: Tzovaras, Bastian Greshake
name: Ebersberger, Ingo
Publisher: name: F1000Research
Year: 2017

description: Newly sequenced genomes, even of species from the remotest corner of the organismic phylogeny, emerge almost on a daily basis. Phylogenetic profiles, capturing the presence and absence of orthologs across species, form the basal information layer for annotating and integrating this novel data. However, these profiles are increasingly overlaid with complementary data such as the extent of sequence similarity between orthologs, similarities and differences of their domain architectures, their Gene Ontology-term-based semantic similarities, or their distribution in a particular phylogenetic clade. A comprehensive tracing of proteins and their functionalities across species and through time requires an intuitive integration optimally of all these various layers. Yet, there are few, if any, visualization tools that aid in this process.

Here, we present PhyloProfile, an intuitive tool for integrating, visualizing and exploring multi- layered phylogenetic profiles. Alongside the presence/absence pattern of orthologs across large taxon collections, the current version of PhyloProfile allows the integration of any two additional information layers. Taxa can be dynamically collapsed into higher order systematic groups, as defined by the NCBI taxonomy. By that the user can rapidly change the resolution from the comparative analyses of proteins in individual species to that of entire kingdoms or even domains without the need of input data modification. Profiles can be filtered dynamically according to various criteria. For example, setting a minimal threshold for the fraction of species in a systematic group having a particular ortholog present can reduce the impact of spurious ortholog identification on evolutionary interpretations. Likewise, filtering collections of orthologs based on the pre-computed similarity of their domain architectures – if provided as an information layer – can either highlight or blend out orthologs with deviating architectures. Subsequent to filtering, the data can be downloaded to serve as input for downstream analyses. Profile plots can be exported as ready-to-publish PDF images with many options for editing their presentation like color, size, position of plot’s axis and legend.

We exemplify the versatility of PhyloProfile by analyzing the phylogenetic profiles of 1605 Microsporidia proteins in 230 species from all three domains of life. We demonstrate how the tool can be applied for (i) estimating gene ages, (ii) tracing changes in protein function approximated by changes in their domain architecture, and (iii) extracting a phylogenomic data set to explore deep splits in the eukaryotic tree of life.

PhyloProfile is provided as an online tool at https://phyloprofile.shinyapps.io/phyloprofile/. The open source code can be downloaded at https://github.com/BIONF/phyloprofile.
Von der Strasse bey Steinsberg nach Guarda
Creators: name: Müller, Johann
Publisher: name: [s.n.]
Year: 1869

description: IM.
description: Gehört zu den Ansichten und Panoramen, die früher als Doubletten bezeichnet wurden. Es handelt sich jedoch um feiner oder weiter ausgearbeitete Zeichnungen, Vorstufen zu Reinzeichnungen oder Sammlungsstücken, die bisher weder katalogisiert noch signiert wurden
description: Vgl. "Gezeichnet an der Strasse bey Steinsberg nach Guarda", Signatur: [Müller-Wegmann] XXVI 6
description: Aus der Sammlung Johann Müller-Wegmann; Depositum der SAC Sektion Uto in der Zentralbibliothek des SAC
Megamelus Fieber 1866
Creators: name: Emeljanov, Alexandr F.
Publisher: name: Zenodo
Year: 2019
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hemiptera
Delphacidae
Megamelus

description: Genus Megamelus Fieber, 1866
Megamelus notula (Germar, 1830)
Note. Apparently, Ding (2006, 209, p. 393) illustrated Megamelus flavus Crawford, 1914 under the the name Megamelus notula (Germar). The illustrations are not clear enough but M. flavus is replacing M. notula in Eastern Palaearctic Region (Anufriev & Emeljanov, 1988).
description: Published as part of Emeljanov, Alexandr F., 2019, New synonyms and new combinations for some Delphacidae (Hemiptera, Auchenorrhyncha) of Eastern and Central Palaearctic Region, pp. 396-400 in Zootaxa 4559 (2) on page 399, DOI: 10.11646/zootaxa.4559.2.13, http://zenodo.org/record/2626956
Effect of both PD-1/PD-L1 and ERK inhibitor on triple negative breast cancer cell lines and the non-triple negative cell line MCF-7
Creators: name: Kabore, Elodie
name: Köster, Frank
name: Polack, Stephan
name: Rody, Achim
name: Bräutigam, Karen
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: Objectives: Diagnosis of triple negative breast cancer (TNBC) is associated with adverse prognosis. The goal was to identify a novel therapy approach for treatment of TNBC cell lines by combination of a PD-1/PD-L1 and an ERK inhibitor.

Methods: After single and combined inhibitor treatment the concentration of 50 % reduction of cell viability (IC50) was determined in three TNBC cell lines and the non-TNBC cell line MCF-7 by MTS assay. Impact on Jak/Stat and MAP kinase pathway and apoptosis induction by inhibitor was checked by immunoblot. QPCR experiments were performed to prove expression ratios of PD-L1, CDK5, Notch1, IL-8, IL-6, CXCL1 und CXCR2 after treatment.

Results: The PD-L1/PD-1 inhibitor provoked 50 % reduction of cell viability with concentrations of 1.26 to 2.25 μM in TNBC and 3.20 μM in MCF-7. For the ERK inhibitor IC50 values were determined of 4.42 to 6.03 μM in TNBC and of 20.44 μM in MCF-7. Combined treatment effected a synergistic or additive inhibition of proliferation. The MAP kinase ERK and S6 were dephosphorylated and expression of Stat3 was increased by ERK inhibitor. Gene expression of IL6 and CXCR2 were repressed by combined treatment.

Conclusion: Though the use of the PD-1/PD-L1 inhibitor and the ERK inhibitor showed contrary effect on phosphorylation of various proteins of the Map kinase signaling pathway, the combined treatment provoked a synergistic or at least additive inhibition of proliferation especially in TNBC and a reduced expression of genes involved in inflammatory processes.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)
Additional file 2 of Patient experiences of buprenorphine dispensing from a mobile medical unit
Creators: name: Messmer, Sarah E.
name: Elmes, Abigail T.
name: Infante, Alexander F.
name: Patterson, Anna
name: Smith, Mackenzie
name: Murphy, Albert Leon
name: Jimenez, Antonio D.
name: Mayer, Stockton
name: Watson, Dennis P.
name: Whitfield, Kevin
name: Fisher, Steven J.
name: Jarrett, Jennie B.
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Cell Biology
subject: Pharmacology
subject: Biotechnology
subject: Sociology
subject: FOS: Sociology
subject: Environmental Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Science Policy
subject: Mental Health
subject: Cardiology

description: Supplementary Material 2
Piz Linard
Creators: name: Müller, Johann
Publisher: name: [s.n.]
Year: 1867

description: IM
description: Gehört zu den Ansichten und Panoramen, die früher als Doubletten bezeichnet wurden. Es handelt sich jedoch um feiner oder weiter ausgearbeitete Zeichnungen, Vorstufen zu Reinzeichnungen oder Sammlungsstücken, die bisher weder katalogisiert noch signiert wurden
description: Aus der Sammlung Johann Müller-Wegmann; Depositum der SAC Sektion Uto in der Zentralbibliothek des SAC
Sonnenfleckenzeichnung Nr. 2065.
Zeichnung von Flecken und Fackelflächen zur Ermittlung der Sonnenaktivität am 24.03.1896, erstellt von Alfred Wolfer
Creators: name: Eidgenössische Sternwarte in Zürich (ETH Zürich bzw. vor 1911 Polytechnikum)
Publisher: name: [s.n.]
Year: 1896

description: Bleistiftzeichnung mit Tinte und Farbstift überschrieben, erstellt als Projektionsbildzeichnung in der Eidgenössischen Sternwarte Zürich. Instrument: Kern-Merz-Refraktor.
Die Beobachtungen zu Einzelflecken und zu Sonnenfleckengruppen wurden mit Bleistift auf einem Vordruck eingetragen. Zugeordnete numerische Werte wurden in einer separat geführten, chronologischen Tabelle erfasst. Unter Umständen sind tabellarisch notierte Werte, die für Protuberanzen mit rot geschrieben wurden, direkt auf der Fleckenzeichnung vermerkt
description: ETH-Bibliothek
Application-oriented Testing and Condition Monitoring of Capacitors in Power Electronic Converters-Slides
Creators: Bo Yao
Publisher: name: IEEE
Year: 2025

PELS Webinar Slides

Abstract:Capacitor is widely used in the DC link and AC filter of power electronic converters applications. It is typically a high-failure component in converter systems and its reliability has become a major focus of attention in recent years. The failure or deterioration of capacitors can lead to a decline in filtering capabilities, which can ultimately lead to the overstressing of components and even the catastrophic failure of the converter system. More realistic testing (i.e., application-oriented) and a better understanding of failure mechanisms and failure modes are key to build-in capacitor reliability in power converter products through design. For availability-critical or safety-critical applications, condition and health monitoring is another reliability engineering tool to reduce unscheduled failure through operation management (i.e., maintenance). Therefore, to address the challenges of capacitor reliability in power electronic converters, this presentation will discuss (1) Robust and Unified Capacitor Stress Emulation Methods;(2) Aging Testing and Failure Analysis of one type of AC Film Capacitors; (3) Non-intrusive Condition Monitoring Methods of Capacitors.
Additional file 1 of Psychometric properties of the maternal breastfeeding evaluation scale: a confirmatory factor analysis
Creators: name: Escribano, Silvia
name: Herrero-Oliver, Raquel
name: Oliver-Roig, Antonio
name: Richart-Martínez, Miguel
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Environmental Sciences not elsewhere classified
subject: Sociology
subject: FOS: Sociology
subject: Cancer
subject: Science Policy

description: Supplementary Material 1
Tumor and host factors regulating anti-tumor immunity and immotherapy efficacy
Creators: name: Gajewski, Thomas F.
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: Two major phenotypes of human melanoma metastases have been observed based on gene expression profiling and confirmatory assays. One subgroup of patients has a T cell-inflamed phenotype that includes expression of chemokines, T cell markers, and a type I IFN signature. In contrast, the other major subset lacks this phenotype and appears to display immune “exclusion”. The mechanisms of immune escape are likely distinct in these two subsets, and therefore the optimal immunotherapeutic interventions necessary to promote clinical responses may be different. The T cell-inflamed tumor microenvironment subset shows the highest expression of negative regulatory factors, including PD-L1, IDO, and FoxP3+ Tregs. Deep analysis of tumor antigen-specific T cells in the tumor microenvironment has identified additional mechanisms of immune dysfunction and new potential therapeutic targets. Treatment strategies targeting several pathways have been translated back into the clinic, with anti-PD-1/PD-L1 agents being FDA approved for 11 different cancer entities. Combinations with new agents based on the biology of T cell dysfunction are ongoing. In contrast to the T cell-inflamed melanomas, non-T cell-inflamed tumors are largely immunotherapy resistant with current approaches. Natural innate immune sensing of tumors appears to occur via the host STING pathway, type I IFN production, and cross-priming of T cells via CD8a+ DCs, and these factors are absent in non-T cell-inflamed tumors. New strategies are being developed to engage or mimic this pathway as a therapeutic endeavor, including STING agonists. The molecular mechanisms that mediate the absence of the T cell-inflamed tumor microenvironment in patients are being elucidated using parallel genomics platforms. The first oncogene pathway identified that mediates immune exclusion is the Wnt/b-catenin pathway, which argues that new pharmacologic strategies to target this pathway should be developed to restore immune access to the tumor microenvironment. Recent evidence has indicated that host factors, including the intestinal microbiota, are also critical. We recently have identified commensal bacteria in mouse models that augment spontaneous anti-tumor immunity and increase efficacy of anti-PD-L1 therapy. Similar analyses in human melanoma patients revealed commensal bacteria associated with anti-PD-1 efficacy. These results have prompted the pursuit of new probiotics that may improve spontaneous immune infiltration and expand immunotherapy efficacy in the clinic.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)
MDSC as a new target for cancer immunotherapy
Creators: name: Umansky, Viktor
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: Tumor microenvironment is characterized by a strong immunosuppression, where myeloidderived suppressor cells (MDSC) induced by chronic inflammation play a major role. Using ret transgenic mouse melanoma model, which mimics clinical situation in human melanoma, we demonstrated MDSC accumulation in melanoma lesions that correlated with reduced anti-tumor T cell reactivity and accelerated tumor progression. The accumulation and activation of MDSC could be mediated not only by soluble inflammatory factors but also by tumor-derived extracellular vesicles, converting immature myeloid cells into immunosuppressive MDSC. Targeting of MDSC migration and functions significantly prolonged survival of tumor-bearing mice associated with the restoration of T cell anti-tumor reactivity. In advanced melanoma patients resistant to immunotherapy with immune checkpoint inhibitors, MDSC targeting induced a beneficial therapeutic effect. We suggest that chronic inflammatory mediators and MDSC are of critical importance for melanoma pathogenesis and their neutralization should be included in combined melanoma immunotherapies to increase their efficiency.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)
Neo-adjuvant checkpoint inhibition – the pathway towards personalized immunotherapy
Creators: name: Blank, Christian
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: Targeted and immunotherapy have revolutionized the systemic and adjuvant therapy in late stage melanoma. Recent pre-clinical and clinical data indicate that neoadjuvant immunotherapy might be superior to adjuvant therapy. Neoadjuvant therapy can bear several advantages, allowing one to 1) determine therapy efficacy within the individual patient for possible additional adjuvant therapy, if needed, 2) reduce tumor burden before surgery, 3) utilize pathological response data as surrogate outcome markers for relapse free and overall survival, and 4) identify biomarkers in a homogenous patient population. For these reasons (except latter), neoadjuvant therapy has become a standard of care in high tumor burden breast cancer. In the case of T cell checkpoint blockade, neoadjuvant therapies could bear a fifth, and potentially significant, advantage. T cell checkpoint blocking antibodies enhance T-cell activation at the moment antigen is encountered, and drug exposure during the time the in-situ malignancy still provides a major source of antigen may therefore potentially induce a stronger and broader tumor-specific T cell response. I will present pre-clinical and clinical data supporting this notion, present early clinical data supporting these ideas and will give an outline for effective biomarker identification, that will lead towards personalized combination immunotherapies.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)
Interference between tumor mutational burden, HPV status, immune signatures and outcome in patients with head and neck cancer treated with chemoradiotherapy: Results from a multicenter retrospective study of the German Cancer Consortium Radiation Oncology
Creators: name: Tinhofer, I.
name: Eder, T.
name: Hess, A. K.
name: Fleischer, V.
name: Balermpas, P.
name: Rödel, C.
name: Linge, A.
name: Krause, M.
name: Baumann, M.
name: Stuschke, M.
name: Zips, D.
name: Grosu, A. L.
name: Abdollahi, A.
name: Debus, J.
name: Ganswindt, U.
name: Belka, C.
name: Pigorsch, S.
name: Multhoff, G.
name: Combs, S. E.
name: Budach, V.
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: Tumor mutational burden as well as extent and quality of tumor immune-cell infiltration have previously been established as biomarkers of efficacy in immune checkpoint inhibitor trials. Their value for predicting outcome after concurrent chemoradiation, a treatment regimen currently evaluated for combination with immune checkpoint inhibitors remains largely unknown. In the talk, the results from a recent multicenter biomarker study of the prognostic role of tumor mutational burden, immune expression profiles and the HPV status in locally advanced head and neck squamous cell carcinoma treated with definitive chemoradiation will be presented. The value of the estimation of tumor mutational burden by targeted versus whole exome sequencing will be discussed. Results of an interference of tumor mutational burden with immune signatures determined by the nanoString PanCancer Immune panel analysis will be presented. Finally, the associations of tumor mutational burden with patient outcome parameters will be presented.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)
Dynamics of conversation: Immunotherapy meets tumor microenvironment
Creators: name: Helfrich, Iris
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: The recent exciting success of cancer immunotherapy has emphasized the essential role of dynamic interactions between tumor cells and the immune system. Inflammation and massive infiltration of leukocytes is a hallmark of various tumor entities and recruitment of immune cells into the tumor microenvironment has been shown to contribute to patients’s clinical outcome in certain types of cancer. In contrast to conventional cancer therapies, such as radiation and chemotherapy, the immunotherapeutic approach specifically targets the different subtypes of immune cells and thus orchestrates a systemic response against tumors. However, depending on the context of the reciprocal interaction between cancer cells their surrounded stromal composition, the tumor microenvironment can both, dampen or enhance immune responses, directing patient’s survival.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)
Additional file 2 of Psychometric properties of the maternal breastfeeding evaluation scale: a confirmatory factor analysis
Creators: name: Escribano, Silvia
name: Herrero-Oliver, Raquel
name: Oliver-Roig, Antonio
name: Richart-Martínez, Miguel
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Environmental Sciences not elsewhere classified
subject: Sociology
subject: FOS: Sociology
subject: Cancer
subject: Science Policy

description: Supplementary Material 2
Isselicrininae Klikushin 1977
Creators: name: Roux, Nadia Améziane Marc Eléaume Michel
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Echinodermata
Crinoidea
Isocrinida
Balanocrinidae

description: Subfamily Isselicrininae Klikushin, 1977
Remark: Klikushin (1977, 1992) included in this subfamily the extinct genera Austinocrinus, Doreckicrinus, Isselicrinus (= Buchicrinus), and Praeisselicrinus, the latter is known from pluricolumnals only. Simms (1988) raised it to family rank and also referred the extant genera Cenocrinus, Endoxocrinus, Annacrinus, Diplocrinus, Metacrinus, and Saracrinus to it. Here, we maintain Isselicrininae as a subfamily rank and of extant genera include only Panglaocrinus gen. nov. (see Discussion below).
description: Published as part of Roux, Nadia Améziane Marc Eléaume Michel, 2024, Classification of Isocrinida (Echinodermata: Crinoidea) with the description of a new extant genus and species from the western Pacific, pp. 994-1012 in Zoological Journal of the Linnean Society (Zool. J. Linn. Soc.) (Zool. J. Linn. Soc.) 200 (4) on page 996, DOI: 10.1093/zoolinnean/zlad101, http://zenodo.org/record/11240631
Modulating the tumor microenvironment
Creators: name: Jäger, Dirk
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: Immunotherapy works in some patients, in most patients it does not. We have tumor entities that show low objective response rates like CRC or PDAC. We identified the immunosuppressive environment being the main mechanisms of resistance. Therefore strategies that target such suppressive mechanisms are being developed clinically. We currently evaluate CCR5 blockade in combination with checkpoint inhibition in MSS-CRC and PDAC.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)
Rediscovering an old story: HLA class I alterations and immune escape of tumors in the context of immunotherapies
Creators: name: Seliger, Barbara
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: Despite the successful implementation of immunotherapies, the response rates of tumor patients are still limited and often resistances develop. This might be a result of the immune suppressive nature of the tumor microenvironment as well as the immune escape mechanisms of tumor cells. While several tumor infiltrating immune cell subpopulations can foster tumor development, exhibit immune suppressive activity and decrease the efficacy of effector immune cells, tumor cells have also developed multiple mechanisms to escape immune recognition and to modulate immune cell function. These include in particular abnormalities in the classical HLA class I antigen, the interferon (IFN) signal transduction pathways and overexpression of non-classical HLA antigens as well as of immune checkpoint molecules. These different strategies have recently been shown to be crucial for the efficacy of immunotherapies against solid tumors and might be associated with the acquired resistances to these treatment options. Thus, a deeper understanding of the key immune players and regulatory pathways of tumor cells involved in the complexity and dynamic interaction between tumor and immune cells might be important for the identification of prognostic factors and the reversion of the immune escape processes. This might lead to increased immune responses against cancer cells and avoidance of immune resistances in order to improve the efficacy of immunotherapies. HLA class I abnormalities frequently occur in tumors, which are associated with a worse prognosis of patients. These defects are mainly due to deregulation rather than structural alterations of HLA class I antigens and antigen processing machinery (APM) components. Reversion of loss or downregulated HLA class I APM component expression could be obtained by cytokine treatment, in particular IFN-, but also by epigenetic drugs, proteoglycans, and anti-oxidant substances as well as microRNAs and RNA-binding molecules. Thus, the control of HLA class I APM expression is highly complex and this novel knowledge could be used for the design of immunotherapeutic approaches to enhance treatment efficacy.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)
The Spokesman - Friday, 10-5-2011
Creators: name: Team, The Editorial
Publisher: name: Morgan State University
Year: 2011
Subjects: NCNW Initiatives
Modern Dance Ensemble
Freshman Class Elections
Peace Corps at Morgan State
Sports

description: NCNW Initiatives: The National Council of Negro Women (NCNW) at Morgan State University focuses on two main initiatives: combating texting while driving and supporting the homeless with adequate winter clothing. The efforts include raising awareness, collecting petitions, and organizing clothing drives to support local shelters​. Modern Dance Ensemble: The Modern Dance Ensemble at Morgan State University celebrates its 35th anniversary. Under the artistic direction of Dr. lantha Tucker, the ensemble has been a prominent part of the university's performance art community. The anniversary includes various performances and events throughout the academic year​ifying the class through community service programs and social events​ NFL Season Highlights: The article discusses the 2011 NFL season, highlighting significant events such as Peyton Manning's contract extension and injury, and the return of the NFL after the lockout. The Baltimore Ravens are mentioned as a team to watch this season. NBA Exhibition Game: Morgan State University hosted an NBA Summer League Exhibition game featuring prominent players like Carmelo Anthony, LeBron James, and Kevin Durant. This event provided a unique opportunity for students and local fans to watch their favorite NBA stars in action​. Peace Corps at Morgan State: Information about the Peace Corps' presence at Morgan State University, including an upcoming information table event, encourages students to learn more about opportunities to serve and make an impact both locally and globally​. Sports coverage
Panglaocrinus Roux 2024, gen. nov.
Creators: name: Roux, Nadia Améziane Marc Eléaume Michel
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Echinodermata
Crinoidea
Isocrinida
Balanocrinidae
Panglaocrinus

description: Genus Panglaocrinus gen. nov.
Type species: Panglaocrinus isseliformis gen. nov., sp. nov..
Etymology: In allusion to the specimen having been collected during the deep-sea cruise PANGLAO 2005.
Diagnosis: As for type-species (see below).
description: Published as part of Roux, Nadia Améziane Marc Eléaume Michel, 2024, Classification of Isocrinida (Echinodermata: Crinoidea) with the description of a new extant genus and species from the western Pacific, pp. 994-1012 in Zoological Journal of the Linnean Society (Zool. J. Linn. Soc.) (Zool. J. Linn. Soc.) 200 (4) on page 996, DOI: 10.1093/zoolinnean/zlad101, http://zenodo.org/record/11240631
Integrin β4 synergizes with E-and P-selectin to regulate tumor growth: Unravelling the molecular mechanisms
Creators: name: Hanika, Sandra
name: Labitzky, Vera
name: Gebauer, Florian
name: Wicklein, Daniel
name: Maar, Hanna
name: Gosau, Tobias
name: Schröder-Schwarz, Jennifer
name: Linder, Stefan
name: Riecken, Kristoffer
name: Schumacher, Udo
name: Lange, Tobias
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: Purpose/Objectives: Metastases are responsible for 90% of cancer-related deaths. Scientific evidence has accumulated showing that adhesion molecules including selectins and integrins play a crucial role in the process of cancer metastasis. In particular, upregulation of integrin β4 (ITGB4) is associated with tumor progression in various tumor entities. Thus, we aimed at detecting a potential crosstalk between selectins and integrins in tumor formation and metastatic spread.


Material/Methods: Knockdown of ITGB4 in PC3 (prostate cancer), PaCa5061 (pancreatic cancer) and SKOV3 (ovarian cancer) cells using shRNA, proliferation and colony forming assays, ELISA, WB, 3D chemotaxis assays with human macrophages, tumor initiation assays in vivo, immunohistochemistry


Results: Knockdown of ITGB4 affected prostatic PC3, pancreatic PaCa5061 and ovarian SKOV3 cancer xenografts already at the primary tumor site. In particular, tumor growth was
strongly inhibited when the ITGB4-knockdown was combined with E-/P-selectin deficiency of mice while selectin-knockout alone had no effect. In this study we wanted to investigate the molecular mechanisms underlying this synergistic growth retardation. Delayed tumor growth was associated with increased apoptosis. Immunostainings further revealed an enhanced immune cell infiltration in ITGB4-knockdown tumors grown in immunodeficient mice. On the contrary, leukocytes were mainly located at the outer tumor periphery of both ITGB4-knockdown and control tumors in E-/P-selectin-knockout mice. The increased leukocyte infiltration in ITGB4-knockdown tumors in control mice seemed to result from a greater chemotactic potential of ITGB4-knockdown cells. CCL2, 5 and 20 were identified as potential chemotactic factors upregulated upon ITGB4-knockdown in PaCa5061 and SKOV3 cells while the chemoattractant playing a role in ITGB4-knockdown PC3 cells remains to be determined.


Conclusion: Taken together, our results suggest that ITGB4-knockdown tumor initiation depends on pro-tumoral signals from attracted tumor-associated leukocytes. These leukocytes appear to depend on endothelial selectins for infiltration of the tumor stroma. Investigation of how leukocytes actually stabilize the survival of ITGB4-knockdown xenografts is the subject of ongoing experiments.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)
Additional file 3 of Psychometric properties of the maternal breastfeeding evaluation scale: a confirmatory factor analysis
Creators: name: Escribano, Silvia
name: Herrero-Oliver, Raquel
name: Oliver-Roig, Antonio
name: Richart-Martínez, Miguel
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Environmental Sciences not elsewhere classified
subject: Sociology
subject: FOS: Sociology
subject: Cancer
subject: Science Policy

description: Supplementary Material 3
The Spokesman - Friday, 11-02-2011
Creators: name: Team, The Editorial
Publisher: name: Morgan State University
Year: 2011
Subjects: Editor's Note
One of Morgan’s Own Launches Publication
Bearly Poets

description: Editor's Note: This issue combines the October 19th edition with the November 2nd edition. The October 19th edition covers Homecoming 2011 and the welcome of the new university president, Dr. David Wilson. The November 2nd edition addresses the new Student Center, rising tuition rates in Maryland, and pays tribute to Julian Benson, Mr. Morgan 2009-2010, who recently passed away. One of Morgan’s Own Launches Publication: J. Cole, a Morgan State University alumnus, successfully launches his publication. Bearly Poets: Bearly Poets, a spoken-word club at Morgan State University, provides a platform for students to express themselves through poetry. Founded by Sunny Williams, the club aims to improve members' grammar, communication skills, and professionalism. The club's inspiration came from UPS (Underground Poetry Spot) at Syracuse University.
Pentacrinitidae Gray 1842
Creators: name: Roux, Nadia Améziane Marc Eléaume Michel
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Echinodermata
Crinoidea
Isocrinida
Pentacrinitidae

description: Family Pentacrinitidae Gray, 1842 (sensu Gislén, 1924)
Lozenge-shaped cirrals, narrow and long areolas in stalk symplexies, absence of cryptosymplexies in stalk.
description: Published as part of Roux, Nadia Améziane Marc Eléaume Michel, 2024, Classification of Isocrinida (Echinodermata: Crinoidea) with the description of a new extant genus and species from the western Pacific, pp. 994-1012 in Zoological Journal of the Linnean Society (Zool. J. Linn. Soc.) (Zool. J. Linn. Soc.) 200 (4) on page 1009, DOI: 10.1093/zoolinnean/zlad101, http://zenodo.org/record/11240631
Primary and recurrent head and neck squamous carcinomas are strikingly different regarding their immune microenvironment
Creators: name: Watermann, Christian
name: Pasternack, Helen
name: Idel, Christian
name: Perner, Sven
name: Krupar, Rosemarie
name: Ribbat-Idel, Julika
name: Kühnel, Mark Philipp
name: Jonigk, Danny
name: Brägelmann, Johannes
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: Purpose/Objectives: The tumor immune microenvironment (TIME) has a crucial impact on cancer progression and patients´ survival for various cancer entities. In head and neck squamous cell carcinoma (HNSCC) the development of recurrences in up to 60% is one main factor leading to poor prognosis. We aimed to reveal changes in the TIME of primary tumours and their corresponding recurrences, hypothesising that recurrent HNSCC have successfully escaped anti-tumor immune response.


Materials/Methods: The TIME of 70 formalin fixed paraffin embedded (FFPE) tissue samples of HNSCC primary tumours and their corresponding local recurrences was characterised via immunohistochemical staining for the markers CD4, CD8, CD20, FOXP3, CD1A, PD1, CD68 and CD56 and semi-automated quantification with Definines software. RNA was extracted using the Maxwell® 16 LEV RNA FFPE Purification Kit for a subgroup of 18 patients, who underwent (chemo-)radiation therapy between the resection of the primary tumour and the development of a local recurrence. RNA expression levels of 770 immune related genes were identified by nanostring nCounter® PanCancer Immune Profiling Panel and assessed with the nSolver™ Analysis Software.


Results: The immunohistochemical TIME analysis demonstrated a loss of CD20+ B lymphocytes (p= 0.0006), an increase in CD1A+ dendritic cells (p= 0.017) and a decrease in the CD8/FOXP3 T-cell ratio (p= 0.106) in HNSCC recurrences compared to their corresponding primary tumours. Nanostring analysis confirmed the depletion of B lymphocytes and additionally revealed a strong decrease in the total number of tumor infiltrating lymphocytes (TILs) as well as an increase in dendritic cells, mast cells, neutrophils and macrophages in HNSCC recurrences. In summary, 141 genes of the nanostring panel were identified to be significantly differentially expressed with the majority being downregulated in the HNSCC recurrences. Significantly downregulated chemokines included CXCL13 and CXCR5, which are involved in B-cell chemotaxis; whereas one of seven upregulated genes, Osteopontin, plays a major role in myeloid cell chemotaxis.


Conclusion: Our results reveal significant differences in the TIME of HNSCC primary tumours and recurrences, characterised by a loss of B lymphocytes and an overall shift from antitumor immune response to an increase in pro-tumor immune factors in recurrences.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)