title: ÐÐ½Ð°Ð»Ð¸Ð· Ð¼Ð¸Ð¾Ð·Ð¸Ð½-9-ÑÐ¾Ð´ÐµÑÐ¶Ð°ÑÐ¸Ñ Ð±ÐµÐ»ÐºÐ¾Ð²ÑÑ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ¾Ð² Ð² ÑÑÐ°ÑÐµÑÑÐ¸Ñ ÐºÐ»ÐµÑÐºÐ°Ñ MSCWJ-1
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Analysis of myosin-9-containing protein complexes in senescent cells MSCWJ-1
Creators: name: ÐÐ¾Ð»ÑÐ½ÑÐºÐ°Ñ, ÐÐ½Ð°ÑÑÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2019
Subjects: Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð°Ð»ÑÐ½ÑÐµ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸
ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ðµ ÑÑÐ°ÑÐµÐ½Ð¸Ðµ
Ð¼Ð¸Ð¾Ð·Ð¸Ð½-9
Ð±ÐµÑÐ°-Ð°ÐºÑÐ¸Ð½
Ð±ÐµÐ»ÐºÐ¾Ð²ÑÐµ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÑ
mesenchymal stem cells
replicative aging
myosin-9
beta-actin
protein complexes

description: Ð Ð°Ð±Ð¾ÑÐ° Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð° Ð½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹ Ð² Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð°ÑÐ¸Ð¸ ÑÐ¾ÐºÑÐ°ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ Ð°Ð¿Ð¿Ð°ÑÐ°ÑÐ° Ð¼ÐµÐ·ÐµÐ½ÑÐ¸Ð¼Ð°Ð»ÑÐ½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð² Ð¿ÑÐ¾ÑÐµÑÑÐµ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ. ÐÐ´Ð½Ð¸Ð¼ Ð¸Ð· ÑÑÑÐµÑÑÐ²ÐµÐ½Ð½ÑÑ Ð¿ÑÐ¸Ð·Ð½Ð°ÐºÐ¾Ð² ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ ÑÐ²Ð»ÑÐµÑÑÑ ÑÐ¼ÐµÐ½ÑÑÐµÐ½Ð¸Ðµ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ Ð¿Ð¾Ð´Ð²Ð¸Ð¶Ð½Ð¾ÑÑÐ¸ Ð¸Ð»Ð¸ Ð¼Ð¸Ð³ÑÐ°ÑÐ¸Ð¸ ÐºÐ»ÐµÑÐ¾Ðº. ÐÐ°ÑÑÑÐµÐ½Ð¸Ðµ Ð¿ÑÐ¾ÑÐµÑÑÐ¾Ð² Ð¼Ð¸Ð³ÑÐ°ÑÐ¸Ð¸ ÑÐ¿Ð¾ÑÐ¾Ð±ÑÑÐ²ÑÐµÑ ÑÑÑÐ´ÑÐµÐ½Ð¸Ñ ÑÐºÐ°Ð½ÐµÐ²Ð¾Ð¹ ÑÐµÐ¿Ð°ÑÐ°ÑÐ¸Ð¸. ÐÐ¾ÑÑÐ¾Ð¼Ñ Ð´Ð»Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ñ ÐÐ¡Ð Ð² ÑÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸Ð²Ð½Ð¾Ð¹ Ð¼ÐµÐ´Ð¸ÑÐ¸Ð½Ðµ Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼Ð¾ Ð·Ð½Ð°ÑÑ ÑÐ°ÑÐ°ÐºÑÐµÑ Ð¿ÑÐ¾ÑÐµÑÑÐ° ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ Ð² ÐºÐ¾Ð½ÐºÑÐµÑÐ½Ð¾Ð¹ Ð»Ð¸Ð½Ð¸Ð¸. ÐÑÐ½Ð¾Ð²Ð½Ð¾Ð¹ ÑÐµÐ»ÑÑ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ ÑÐµÐ¾ÑÐ³Ð°Ð½Ð¸Ð·Ð°ÑÐ¸Ð¸ ÑÐ¾ÐºÑÐ°ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ Ð°Ð¿Ð¿Ð°ÑÐ°ÑÐ° Ð² Ð¿ÑÐ¾ÑÐµÑÑÐµ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ Ð² Ð»Ð¸Ð½Ð¸Ð¸ ÐÐ¡Ð, Ð½ÐµÐ´Ð°Ð²Ð½Ð¾ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½Ð¾Ð¹ Ð¸ Ð¾ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸Ð·Ð¾Ð²Ð°Ð½Ð½Ð¾Ð¹ Ð² Ð½Ð°ÑÐµÐ¹ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ (MSCWJ-1). Ð ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¸ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ñ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð² ÑÐ°ÑÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ð¸ Ð¼Ð¸Ð¾Ð·Ð¸Ð½Ð°-9 Ð¸ Ð²ÑÑÐ²Ð»ÐµÐ½Ñ ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐµÑÐºÐ¸Ðµ Ð¼Ð¸Ð¾Ð·Ð¸Ð½-9-ÑÐ¾Ð´ÐµÑÐ¶Ð°ÑÐ¸Ðµ ÑÐ¸ÑÐ¾Ð¿Ð»Ð°Ð·Ð¼Ð°ÑÐ¸ÑÐµÑÐºÐ¸Ðµ Ð±ÐµÐ»ÐºÐ¾Ð²ÑÐµ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÑ Ð² ÐºÐ»ÐµÑÐºÐ°Ñ Ð½Ð° ÑÑÐµÐ´Ð½Ð¸Ñ Ð¿Ð°ÑÑÐ°Ð¶Ð°Ñ (15 â 20). Ð¢Ð°ÐºÐ¶Ðµ Ð±ÑÐ»Ð¸ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ñ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð² ÑÐ¾ÑÑÐ°Ð²Ðµ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½ÑÑ Ð¼Ð¸Ð¾Ð·Ð¸Ð½-9- Ð¸ Ð±ÐµÑÐ°-Ð°ÐºÑÐ¸Ð½-ÑÐ¾Ð´ÐµÑÐ¶Ð°ÑÐ¸Ñ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ¾Ð², Ð¿ÑÐ¾Ð¸ÑÑÐ¾Ð´ÑÑÐ¸Ðµ Ð² ÑÐ¾Ð´Ðµ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ ÐºÐ»ÐµÑÐ¾Ðº.
description: The work is aimed at study changes in the organization of the contractile apparatus of human mesenchymal stem cells in the process of replicative aging. One of the essential signs of replicative aging is a decrease in cell motility and migration. The decline of cell motility contributes to the deterioration of tissue repair. Therefore, to use MSCs in regenerative medicine, it is necessary to know the nature of the process of replicative aging in a particular line. The main goal of the work was to study the reorganization of contractile apparatus in the process of replicative aging in the recently obtained and characterized in our laboratory line of MSC (MSCWJ-1). Changes in the distribution of myosin-9 were detected during the study. Specific myosin-9-containing cytoplasmic protein complexes were detected in the cells at medium passages (15-20). Ð¡hanges occurring in the composition of isolated myosin-9- and beta-actin-containing complexes during replicative cell aging also were detected.