title: ÐÐ¾Ð¸ÑÐº Ð¸ Ð°Ð½Ð°Ð»Ð¸Ð· ÑÐ»ÐµÐ¼ÐµÐ½ÑÐ¾Ð² ÑÐ¸ÑÑÐµÐ¼ Ð°Ð½ÑÐ¸ÑÐ°Ð³Ð¾Ð²Ð¾Ð¹ Ð·Ð°ÑÐ¸ÑÑ ÐºÐ»ÐµÑÐºÐ¸ Ð² Ð³ÐµÐ½Ð¾Ð¼Ð°Ñ ÐºÐ»ÑÐ±ÐµÐ½ÑÐºÐ¾Ð²ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ ÑÐµÐ¼ÐµÐ¹ÑÑÐ²Ð° Rhizobiaceae Ð¼ÐµÑÐ¾Ð´Ð°Ð¼Ð¸ Ð±Ð¸Ð¾Ð¸Ð½ÑÐ¾ÑÐ¼Ð°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð³Ð¾ Ð¸ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾-Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Search and analysis of elements of cell antiphage defense systems in the genomes of Rhizobiaceae nodule bacteria by bioinformatic and molecular-biological analysis
Creators: name: ÐÐ¿ÑÐµÐ»ÐºÐ¾Ð²Ð°, ÐÐ½Ð°ÑÑÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: ÐºÐ»ÑÐ±ÐµÐ½ÑÐºÐ¾Ð²ÑÐµ Ð±Ð°ÐºÑÐµÑÐ¸Ð¸
ÑÐ¸ÑÑÐµÐ¼Ñ Ð°Ð½ÑÐ¸ÑÐ°Ð³Ð¾Ð²Ð¾Ð¹ Ð·Ð°ÑÐ¸ÑÑ ÐºÐ»ÐµÑÐºÐ¸
Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð¸
root-nodule bacteria
antiphage defence systems
bacteriophages

description: ÐÐ°Ð´Ð°ÑÐ¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: â Ð¾Ð¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ Ð¸ Ð¾ÑÐµÐ½Ð¸ÑÑ ÑÐ¿ÐµÐºÑÑ Ð¸ ÑÑÑÑÐºÑÑÑÑ ÑÐ¸ÑÑÐµÐ¼ Ð°Ð½ÑÐ¸ÑÐ°Ð³Ð¾Ð²Ð¾Ð¹ Ð·Ð°ÑÐ¸ÑÑ ÐºÐ»ÐµÑÐºÐ¸ Ð² Ð¿Ð¾Ð»Ð½Ð¾Ð³ÐµÐ½Ð¾Ð¼Ð½ÑÑ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ ÑÐµÐ¼ÐµÐ¹ÑÑÐ²Ð° Rhizobiaceae; â Ð¾ÑÐµÐ½Ð¸ÑÑ Ð½Ð°Ð»Ð¸ÑÐ¸Ðµ Ð¸ ÑÑÑÑÐºÑÑÑÐ½ÑÐ¹ Ð¿Ð¾Ð»Ð¸Ð¼Ð¾ÑÑÐ¸Ð·Ð¼ Ð³ÐµÐ½Ð¾Ð² ÑÐ¸ÑÑÐµÐ¼ Ð°Ð½ÑÐ¸ÑÐ°Ð³Ð¾Ð²Ð¾Ð¹ Ð·Ð°ÑÐ¸ÑÑ Ð² ÐºÐ»ÐµÑÐºÐ°Ñ; â Ð¾ÑÐµÐ½Ð¸ÑÑ ÑÐ°Ð·Ð»Ð¸ÑÐ¸Ñ Ð¼ÐµÐ¶Ð´Ñ Ð³ÑÑÐ¿Ð¿Ð°Ð¼Ð¸ Ð³ÐµÐ¾Ð³ÑÐ°ÑÐ¸ÑÐµÑÐºÐ¸ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ Ð¿Ð¾Ð¿ÑÐ»ÑÑÐ¸Ð¹ Ð¿ÑÐ¸ÑÐ¾Ð´Ð½ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð² Sinorhizobium spp; â Ð¿ÑÐ¾Ð²ÐµÑÑÐ¸ ÑÐ¸Ð»Ð¾Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð°Ð½Ð°Ð»Ð¸Ð· Ð½ÑÐºÐ»ÐµÐ¾ÑÐ¸Ð´Ð½ÑÑ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÐµÐ¹ Ð³ÐµÐ½Ð°, ÐºÐ¾Ð´Ð¸ÑÑÑÑÐµÐ³Ð¾ Ð¾Ð´Ð¸Ð½ Ð¸Ð· ÑÐ»ÐµÐ¼ÐµÐ½ÑÐ¾Ð² ÑÐ¸ÑÑÐµÐ¼ Ð°Ð½ÑÐ¸ÑÐ°Ð³Ð¾Ð²Ð¾Ð¹ Ð·Ð°ÑÐ¸ÑÑ ÐºÐ»ÐµÑÐºÐ¸. ÐÐ±ÑÐµÐºÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â ÐºÐ»ÑÐ±ÐµÐ½ÑÐºÐ¾Ð²ÑÐµ Ð±Ð°ÐºÑÐµÑÐ¸Ð¸ ÑÐµÐ¼ÐµÐ¹ÑÑÐ²Ð° Rhizobiaceae. Ð Ð°Ð±Ð¾ÑÐ° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ Ð³ÐµÐ½ÐµÑÐ¸ÐºÐ¸ Ð¸ ÑÐµÐ»ÐµÐºÑÐ¸Ð¸ Ð¼Ð¸ÐºÑÐ¾Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð¾Ð² Ð¤ÐÐÐÐ£ ÐÐÐÐÐ¡Ð¥Ð. Ð Ð°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ ÑÐ°Ð·Ð½Ð¾Ð¾Ð±ÑÐ°Ð·Ð¸Ñ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÐ»ÐµÐ¼ÐµÐ½ÑÐ¾Ð² Ð² Ð³ÐµÐ½Ð¾Ð¼Ð°Ñ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ ÑÐµÐ¼ÐµÐ¹ÑÑÐ²Ð° Rhizobiaceae, Ð²Ð¾Ð²Ð»ÐµÑÐµÐ½Ð½ÑÑ Ð² Ð°Ð½ÑÐ¸ÑÐ°Ð³Ð¾Ð²ÑÑ Ð·Ð°ÑÐ¸ÑÑ ÐºÐ»ÐµÑÐºÐ¸. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ» Ð²ÑÑÐ²Ð»ÐµÐ½ ÑÐ¸ÑÐ¾ÐºÐ¸Ð¹ ÑÐ¿ÐµÐºÑÑ Ð½Ð¾Ð²ÑÑ ÑÐ¸ÑÑÐµÐ¼ Ð°Ð½ÑÐ¸ÑÐ°Ð³Ð¾Ð²Ð¾Ð¹ Ð·Ð°ÑÐ¸ÑÑ ÐºÐ»ÐµÑÐºÐ¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ ÑÐµÐ¼ÐµÐ¹ÑÑÐ²Ð° Rhizobiaceae, Ð±ÑÐ» Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½ ÑÐ¸Ð»Ð¾Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð¸ ÐÐ¦Ð -ÐÐÐ Ð¤ Ð°Ð½Ð°Ð»Ð¸Ð· ÑÑÐ°Ð¼Ð¼Ð¾Ð² Sinorhizobium spp. ÐÑÐ¸ Ð½Ð°Ð¿Ð¸ÑÐ°Ð½Ð¸Ð¸ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±Ð·Ð¾ÑÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ: NCBI, ScienceDirect, Scopus, eLIBRARY.RU, CyberLeninka. Ð¡ÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÑÑ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»Ð¸ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿Ð°ÐºÐµÑÐ° Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼ Microsoft Office Ð¸ Past4.
description: Tasks that were solved in the course of the study: evaluation of the spectrum and structure of cell antiphage defence systems in full genome sequences of the Rhizobiaceae family bacteria; â analysis of the presence and structural polymorphism of genes of antiphage defense systems in cells; â analysis the differences between groups of geographically different populations of natural strains of Sinorhizobium spp.; â perform phylogenetic analysis of the nucleotide sequences of the gene encoding one of the elements of the cells antiphage defense systems. The object of research is root-nodule bacteria of the Rhizobiaceae family. The work was carried out on the basis of the Laboratory of Genetics and Selection of Microorganisms of the All-Russian Research Institute of Agricultural Microbiology. The work is devoted to studying the diversity of genetic elements in the genomes of bacteria of the Rhizobiaceae family, involved in cell antiphage protection. As a result of the work, a wide range of new systems of antiphage protection of Rhizobiaceae bacterial cells was identified, phylogenetic and PCR-RFLP analysis of Sinorhizobium spp. strains was performed. The following databases were used for writing the literature review: NCBI, ScienceDirect, Scopus, and eLIBRARY.RU, CyberLeninka. Statistical processing of experimental data was performed using the Microsoft Office software package and Past4.