Boothiella tetraspora Lodhi &amp; J. H. Mirza
Creators: name: Yang, Erfu
name: Lu, Wenhua
name: Tibpromma, Saowaluck
name: Dai, Dongqin
name: Gao, Ying
name: Promputtha, Itthayakorn
name: Karunarathna, Samantha C.
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Fungi
Ascomycota
Sordariomycetes
Sordariales
Chaetomiaceae
Boothiella
Boothiella tetraspora

description: <i>Boothiella tetraspora</i> Lodhi &amp; J.H. Mirza, Mycologia 54(2): 217 (1962) (Figure 4) Index Fungorum number: IF 327083 Isolated from intestinal contents of dead American bullfrog larvae. <b>Sexual morph on PDA:</b> <i>Mycelium</i> 3–5 μm wide, hyaline to yellow pale, thick-walled, branched, septate, with granules. <i>Ascomata</i> 260–370 × 240–330 μm (x̅ =245 × 285 μm, n=10), globose to subglobose, solitary or gregarious, superficial to immersed on PDA, brown to dark brown or black, membranaceous, glabrous, without ostiolate. <i>Ascomatal wall</i> composed of membranaceous, subhyaline to pale brown cells of <i>textura angularis. Hamathecium</i> not observed. <i>Asci</i> 80–100 × 15–20 μm (x̅ =87.5 × 15.5 μm, n=20), unitunicate, four-spored, clavate to broadly cylindrical, apically round, short pedicellate, furcate pedicel, evanescent. <i>Ascospores</i> 20–30 × 10–20 μm (x̅ = 24 × 14.5 μm, n=20), ovoid to ellipsoidal, hyaline to yellowish brown to dark brown, aseptate, rough to verruculose, sometimes visible a germ pore at one or each end, without sheath. <b>Asexual morph:</b> Undetermined. <b>Culture characteristics:</b> Colonies growing on PDA reach 30–40 mm in diameter after one week at 27 °C, forming the brown to dark brown fruiting bodies within one month in PDA. Obverse: flat, velvety, hairy, pale brown, entire edge. Reverse: yellowish brown. Without pigments produced in PDA. <b>Known substratum:</b> Soil (Lodhi &amp; Mirza 1962, Wang <i>et al.</i> 2019); Sand (Wang <i>et al.</i> 2019); Intestinal contents of dead American bullfrog larvae (this study). <b>Known Distribution:</b> Pakistan (Lodhi &amp; Mirza 1962); Spain, India (Wang <i>et al</i>. 2019), China (This study). <b>Material examined:</b> China, Yunnan Province, Qujing Normal University, intestinal contents of dead American bullfrog larvae, GPS: 103°44’35”E, 25°30’46”N, 1856.6 m, Wen-hua Lu, ER 4, (Herb. HKAS 125767), living culture KUNCC22-12510. <b>Notes:</b> Based on detailed descriptions of <i>Boothiella tetraspora</i>, our isolate KUNCC 22-12510 fits with previous strains in morphology, forming brown to dark brown ascomata, with globose to subglobose, superficial to immersed, non-ostiolate, additionally, both strains almost have the same size of asci and ascospores, with four-spored asci, ovoid to ellipsoidal conidia (Lodhi &amp; Mirza 1962, Wang <i>et al.</i> 2019). Phylogenetically, our isolate KUNCC 22-12510 clustered with <i>Boothiella tetraspora</i> (CBS 334.67 and CBS 887.97) with reliable statistical supports (99% ML /1 BYPP, Figure 2). The BLASTn results of ITS, LSU, <i>tub2,</i> and <i>rpb2</i> region demonstrate 99–100% similarity with <i>Boothiella tetraspora</i> strains (CBS 334.67 and CBS 887.97). Therefore, our isolate KUNCC 22-12510 is identified as <i>B. tetraspora</i>, a new host and country record based on morphological features combined with phylogeny evidence.
description: Published as part of <i>Yang, Erfu, Lu, Wenhua, Tibpromma, Saowaluck, Dai, Dongqin, Gao, Ying, Promputtha, Itthayakorn &amp; Karunarathna, Samantha C., 2023, Three interesting fungi from American bullfrog larvae (Rana catesbeiana) in Yunnan, China, pp. 251-268 in Phytotaxa 587 (3)</i> on pages 258-259, DOI: 10.11646/phytotaxa.587.3.4, http://zenodo.org/record/7744408
description: {"references": ["Lodhi, S. A. &amp; Mirza, R. F. (1962) A new genus of the Eurotiales. Mycologia 54: 217 - 219. https: // doi. org / 10.1080 / 00275514.1962.12024993", "Wang, X. W., Bai, F. Y., Bensch, K., Meijer, M., Sun, B. D., Han, Y. F., Crous, P. W., Samson, R. A., Yang, F. Y. &amp; Houbraken, J. (2019) Phylogenetic re-evaluation of Thielavia with the introduction of a new family Podosporaceae. Studies in Mycology 93: 155 - 252. https: // doi. org / 10.1016 / j. simyco. 2019.08.002"]}