title: Ð£ÑÐ°ÑÑÐ¸Ðµ STAT1 Ð² ÑÐµÐ³ÑÐ»ÑÑÐ¸Ð¸ Ð°Ð¿Ð¾Ð¿ÑÐ¾Ð·Ð° Ð½ÐµÑÐ²Ð½Ð¾Ð¹ ÑÐºÐ°Ð½Ð¸ Ð¿ÑÐ¸ Ð¾ÑÐ°Ð³Ð¾Ð²Ð¾Ð¹ ÑÐ°ÑÐ¼Ð°ÐºÐ¾ÑÐµÐ·Ð¸ÑÑÐµÐ½ÑÐ½Ð¾Ð¹ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
Creators: name: Ð¯ÐºÐ¾Ð²Ð»ÐµÐ²Ð°, ÐÑÐ¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: ÐÐµÐ»ÐºÐ¸
Ð¾ÑÐ°Ð³Ð¾Ð²Ð°Ñ ÑÐ°ÑÐ¼Ð°ÐºÐ¾ÑÐµÐ·Ð¸ÑÑÐµÐ½ÑÐ½Ð°Ñ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ñ
Ð²Ð¸ÑÐ¾ÑÐ½Ð°Ñ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ñ
STAT1
p53
BCl-2
ÐºÐ°ÑÐ¿Ð°Ð·Ð°-3
ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð¾ÑÐ°Ð³

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«Ð£ÑÐ°ÑÑÐ¸ÐµSTAT1 Ð² ÑÐµÐ³ÑÐ»ÑÑÐ¸Ð¸ Ð°Ð¿Ð¾Ð¿ÑÐ¾Ð·Ð° Ð½ÐµÑÐ²Ð½Ð¾Ð¹ ÑÐºÐ°Ð½Ð¸ Ð¿ÑÐ¸ Ð¾ÑÐ°Ð³Ð¾Ð²Ð¾Ð¹ ÑÐ°ÑÐ¼Ð°ÐºÐ¾ÑÐµÐ·Ð¸ÑÑÐµÐ½ÑÐ½Ð¾Ð¹ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ð¸Â». ÐÑÐµÐ´Ð»Ð¾Ð¶ÐµÐ½Ð° Ð¼ÐµÑÐ¾Ð´Ð¸ÐºÐ° Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ ÑÑÐ¾Ð²Ð½Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð±ÐµÐ»ÐºÐ¾Ð², ÑÑÐ°ÑÑÐ²ÑÑÑÐ¸Ñ Ð² Ð°Ð¿Ð¾Ð¿ÑÐ¾Ð·Ðµ Ð½ÐµÑÐ²Ð½Ð¾Ð¹ ÑÐºÐ°Ð½Ð¸ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ ÐÐµÑÑÐµÑÐ½-Ð±Ð»Ð¾ÑÑÐ¸Ð½Ð³Ð°. ÐÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ðµ Ð³ÑÑÐ¿Ð¿Ñ Ð¿Ð°ÑÐ¸ÐµÐ½ÑÐ¾Ð² Ñ Ð¤Ð Ð­ Ð¾ÑÐ½Ð¾ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾ Ð³ÑÑÐ¿Ð¿Ñ ÐºÐ¾Ð½ÑÑÐ¾Ð»Ñ Ð¿Ð¾ ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ñ ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸Ð¾Ð½Ð½Ð¾Ð³Ð¾ ÑÐ°ÐºÑÐ¾ÑÐ° STAT1, Ð° ÑÐ°ÐºÐ¶Ðµ Ð±ÐµÐ»ÐºÐ¾Ð² Ñ53, ÐºÐ°ÑÐ¿Ð°Ð·Ñ-3 Ð¸ Bcl-2. ÐÑÐµÐ´Ð¼ÐµÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: ÑÐ¾Ð»Ñ STAT1 Ð² ÑÐµÐ³ÑÐ»ÑÑÐ¸Ð¸ Ð°Ð¿Ð¾Ð¿ÑÐ¾Ð·Ð° Ð½ÐµÑÐ²Ð½Ð¾Ð¹ ÑÐºÐ°Ð½Ð¸ Ð¿ÑÐ¸ Ð¾ÑÐ°Ð³Ð¾Ð²Ð¾Ð¹ ÑÐ°ÑÐ¼Ð°ÐºÐ¾ÑÐµÐ·Ð¸ÑÑÐµÐ½ÑÐ½Ð¾Ð¹ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ð¸. Ð¦ÐµÐ»Ñ ÑÐ°Ð±Ð¾ÑÑ: Ð¾ÑÐµÐ½Ð¸ÑÑ ÑÐ¾Ð»Ñ ÑÑÐ°Ð½ÑÐºÑÐ¸Ð¿ÑÐ¸Ð¾Ð½Ð½Ð¾Ð³Ð¾ ÑÐ°ÐºÑÐ¾ÑÐ° STAT1, Ð±ÐµÐ»ÐºÐ¾Ð² Ñaspase-3, Bcl-2 Ð¸ Ñ53 Ð² ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ð¸ Ñ53-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾Ð³Ð¾ Ð°Ð¿Ð¾Ð¿ÑÐ¾Ð·Ð° Ð² Ð²Ð¸ÑÐ¾ÑÐ½Ð¾Ð¹ Ð¾Ð±Ð»Ð°ÑÑÐ¸ ÐºÐ¾ÑÑ Ð¸ Ð¿ÑÐ¸Ð»ÐµÐ³Ð°ÑÑÐµÐ³Ð¾ Ð±ÐµÐ»Ð¾Ð³Ð¾ Ð²ÐµÑÐµÑÑÐ²Ð° Ð¼Ð¾Ð·Ð³Ð° ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð² ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¾Ð³ÐµÐ½Ð½Ð¾Ð¼ Ð¾ÑÐ°Ð³Ðµ Ð¿ÑÐ¸ ÑÐ°ÑÐ¼Ð°ÐºÐ¾ÑÐµÐ·Ð¸ÑÑÐµÐ½ÑÐ½Ð¾Ð¹ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸Ð¸. Ð ÐµÐ·ÑÐ»ÑÑÐ°ÑÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°: Ð¾ÑÐ¼ÐµÑÐµÐ½Ð¾ Ð¿Ð¾Ð²ÑÑÐµÐ½Ð¸Ðµ ÑÑÐ¾Ð²Ð½Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ STAT1 Ñ Ð¿Ð°ÑÐ¸ÐµÐ½ÑÐ¾Ð² Ñ Ð¤Ð Ð­, Ð¾ÑÐ¾Ð±ÐµÐ½Ð½Ð¾ Ð² ÑÐ¿Ð¸Ð¾ÑÐ°Ð³Ðµ. B ÑÑÐ¸Ñ Ð¶Ðµ Ð¾Ð±Ð»Ð°ÑÑÑÑ Ð¿Ð¾Ð²ÑÑÐµÐ½Ð¾ ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ðµ Ð±ÐµÐ»ÐºÐ°Ñ53 âÐ°ÐºÑÐ¸Ð²Ð°ÑÐ¾ÑÐ° Ð°Ð¿Ð¾Ð¿ÑÐ¾Ð·Ð°. Ð£ÑÐ¾Ð²ÐµÐ½Ñ Ð°Ð½ÑÐ¸Ð°Ð¿Ð¾Ð¿ÑÐ¾ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° Bcl-2 Ð² ÑÐºÐ°Ð½ÑÑ Ð¼Ð¾Ð·Ð³Ð° Ð¿Ð°ÑÐ¸ÐµÐ½ÑÐ¾Ð² Ñ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸ÐµÐ¹ Ð¿Ð¾Ð²ÑÑÐµÐ½ Ð¿Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ Ñ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½Ð¾Ð¹ Ð³ÑÑÐ¿Ð¿Ð¾Ð¹. ÐÑÐ²Ð¾Ð´Ñ: Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ ÑÑÐ¾Ð²Ð½Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð²ÑÑÐµÑÐºÐ°Ð·Ð°Ð½Ð½ÑÑ Ð±ÐµÐ»ÐºÐ¾Ð² ÑÐºÐ»Ð°Ð´ÑÐ²Ð°ÐµÑÑÑ Ð² Ð¾Ð±ÑÑÑ ÐºÐ°ÑÑÐ¸Ð½Ñ Ð½ÐµÐ¹ÑÐ¾Ð½Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð°Ð¿Ð¾Ð¿ÑÐ¾Ð·Ð° Ð¿ÑÐ¸ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¾Ð³ÐµÐ½ÐµÐ·Ðµ Ð¸ Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÐµÑ Ð¿ÑÐµÐ´Ð¿Ð¾Ð»Ð¾Ð¶Ð¸ÑÑ, ÑÑÐ¾ Ð¾Ð½Ð¸ Ð¸Ð³ÑÐ°ÑÑ Ð²Ð°Ð¶Ð½ÑÑ ÑÐ¾Ð»Ñ Ð² ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð¾Ð² ÑÐ°ÑÐ¼Ð°ÐºÐ¾ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾ÑÑÐ¸. ÐÐ±Ð»Ð°ÑÑÑ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð² Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ:ÐµÑÐ»Ð¸ Ð·Ð½Ð°ÑÐµÐ½Ð¸Ðµ STAT ÑÐ°ÐºÑÐ¾ÑÐ¾Ð² ÐºÐ°Ðº ÑÐµÐ³ÑÐ»ÑÑÐ¾ÑÐ¾Ð² Ð°Ð¿Ð¾Ð¿ÑÐ¾Ð·Ð° Ð² Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐµÐ¼ Ð±ÑÐ´ÐµÑ Ð¿Ð¾Ð´ÑÐ²ÐµÑÐ¶Ð´ÐµÐ½Ð¾, ÑÐ¾ ÑÑÐ¾ Ð¾ÑÐºÑÐ¾ÐµÑ ÑÐ¸ÑÐ¾ÐºÐ¸Ðµ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾ÑÑÐ¸ Ð´Ð»Ñ Ð¿ÑÐ¸Ð½ÑÐ¸Ð¿Ð¸Ð°Ð»ÑÐ½Ð¾ Ð½Ð¾Ð²Ð¾Ð³Ð¾ Ð¿Ð¾Ð´ÑÐ¾Ð´Ð° Ðº Ð±Ð¾ÑÑÐ±Ðµ Ñ ÑÐ°ÑÐ¼Ð°ÐºÐ¾ÑÐµÐ·Ð¸ÑÑÐµÐ½ÑÐ½Ð¾Ð¹ ÑÐ¿Ð¸Ð»ÐµÐ¿ÑÐ¸ÐµÐ¹ Ð¸ ÑÐµÐ¼ ÑÐ°Ð¼ÑÐ¼ Ð¿Ð¾Ð·Ð²Ð¾Ð»Ð¸Ñ ÑÐ»ÑÑÑÐ¸ÑÑ ÐºÐ°ÑÐµÑÑÐ²Ð¾ Ð¶Ð¸Ð·Ð½Ð¸ ÑÐµÑ Ð¿Ð°ÑÐ¸ÐµÐ½ÑÐ¾Ð², Ð´Ð»Ñ ÐºÐ¾ÑÐ¾ÑÑÑ Ð¼ÐµÐ´Ð¸ÐºÐ°Ð¼ÐµÐ½ÑÐ¾Ð·Ð½Ð¾Ðµ Ð»ÐµÑÐµÐ½Ð¸Ðµ Ð½Ðµ ÑÐ²ÐµÐ½ÑÐ°Ð»Ð¾ÑÑ ÑÑÐ¿ÐµÑÐ¾Ð¼.
description: The subject of the graduate qualification work is Â«Participation of STAT1 in the regulation of apoptosis of nervous tissue in focal pharmacoresistant epilepsyÂ». This paper describes a method for determining the level of expression of proteins involved in apoptosis of nervous tissue using Western blotting technique. A comparison group of patients with DRE relative to the control group on the content of the STAT1 transcription factor and protein p53, caspase-3 and Bcl-2.Subject of research: the role of STAT1 in the regulation of apoptosis of the nervous tissue in focal drug-resistant epilepsy.Purpose of the work: to assess the role of the transcription factor STAT1, caspase-3, Bcl-2, and p53 proteins in the development of p53-dependent apoptosis in the temporal cortex and adjacent white matter of the human brain in an epileptogenic focus in drug-resistant epilepsy.Experimental results: there was increase in the level of STAT1 expression in patients with DRE, especially in the epio focus. In the same areas, the content of p53 -a direct activator of apoptosis -is increased. The level of the antiapoptotic protein Bcl-2 in the brain tissues of patients with epilepsy is increased compared to the control group.Conclusions: the change in the expression level of the above proteins fits into the general picture of neuronal apoptosis during epileptogenesis and suggests that they play an important role in the formation of mechanisms of pharmacoresistance.The field of application of the study results: if the significance of STAT factors as regulators of apoptosis is furtherconfirmed, this will open up wide opportunities for a fundamentally new approach to combating drug-resistant epilepsy and thereby improve the quality of life of those patients for whom drug treatment has not been successful.