cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Wirkung von N-Trichlormethylthio-tetrahydrophthalimid auf Weinhefen
Creators: name: Drobnica, L.
name: Šturdík, E.
name: Minárik, E.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2016

description: N-Trichlormethylthio-tetrahydrophthalimid (NTT) ist in mehreren käuflichen Fungiziden enthalten, die im Rebschutz verwendet werden. Diese Substanz ist infolge der Inaktivierung einiger glykolytischer Enzyme als wirksamer Inhibitor der Weinhefengärung anzusprechen. Versuchergebnisse mit Atmungs-Hefentypen weisen auch auf Eingriffe von NTT an verschiedenen Stellen der oxidativen Phosphorylierung hin. Die Inhibition dieses Vorganges sowie der Glykolyse beruht auf einer Abänderung der Proteine, die für ihre katalytische Aktivität Thiolgruppen benötigen. Als Folge mehrfacher Eingriffe von NTT in den energetischen Stoffwechsel der Hefen kommt es zur Hemmung biosynthetischer Prozesse und damit auch zur Einstellung des Zellwachstums. Hierauf deuten die Ergebnisse von Untersuchungen über die Aufnahme 14C-markierter Präkursoren der Protein- und Nukleinsäuresynthese hin. Es werden ferner Angaben mitgeteilt, die es ermöglichen, die Hemmwirkung verschiedener Fungizidpräparate des Handels gegenüber Hefen zu vergleichen. Die Bedeutung der Hefen als Modellorganismen zur Aufklärung des Wirkungsmechanismus fungitoxischer Substanzen wird unterstrichen. Effect of N-trichloromethylthio-tetrahydropbtbalimide on wine yeasts N-trichloromethylthio-tetrahydrophthalimide (NTT), an active component of many commercial fungicidal preparations used in viticulture, is a powerful in hibitor of yeast fermentation due to the inactivation of some glycolytic enzymes. In experiments especially with respiratory yeast types the results obtained show also interferences of NTT with the oxidative phosphorylation. The inhibition of this process and also of glycolysis is a result of modificaticn of proteins requiring thiol groups for their catalytic activity. As a consequence of the multitarget effect of NTT on yeast energy metabolism, biosynthetic processes are inhibited and, finally, yeast growth is blocked. These facts are indicated by the results obtained from study of NTT influence on incorporation of 14Clabelled precursors of both protein and nucleic acid biosynthesis. Data included in the paper make it also possible to compare the antiyeast activities of different types of commercial fungicides. The importaRce of yeasts as a model for the elucidation of the mode of action of antifungal substances is emphasized.
description: VITIS - Journal of Grapevine Research, Vol. 19 No. 1 (1980): Vitis
title: Seasonal Distribution and the Retention of Some Decapod Crustacean Larvae Within the Chesapeake Bay, Virginia
Creators: name: Goy, Joseph William
Publisher: name: Old Dominion University
publisherIdentifier: https://ror.org/04zjtrb98
publisherIdentifierScheme: ROR
Year: 1976

description: Planktonic stages or 45 genera and species or decapod larvae were identified and staged from samples taken over a three year period in the lower portion and entrance area or Chesapeake Bay. The distribution of each larval stage of the decapod crustaceans found in the bay was determined and from this, the extent of the respective breeding season was deduced. In 1975, samples were taken monthly from stations along a transect at the baymouth, both at the surface and bottom. Two stations were also occupied over the tidal cycle in daylight and darkness. Evidence is presented that larvae of some typically offshore decapods can be retained in the estuary. Larvae tended to occur in the bottom layers the majority of the times, thereby being retained within the estuary. Results from the overnight stations showed the larvae underwent a greater vertical migration in response to tidal flow than in response to changes in illumination.
Sensitivity of the damage response and fracture path to material heterogeneity present in a sandstone specimen containing a pre-existing 3-D surface flaw under uniaxial loading
Creators: name: Mondal, S.
name: Olsen, Louise
name: Gross, L.
Publisher: name: Swinburne
Year: 2024

description: The heterogeneity of natural rock affects its fracture behaviour resulting in a variation of rock strength. To investigate the sensitivity of damage response and failure strength across rock samples numerical simulations are performed with random realizations of the Young's modulus. As a test case, we consider a rock specimen with a pre-existing 3-D surface flaw under uniaxial compression. To test the sensitivity of the damage response due to material heterogeneity for specific geometrical configurations Young's modulus is varied at the mesoscale using different randomized distributions while keeping the bulk material properties constant at the macroscale. The study shows that in general, the standard deviation is independent from geometrical parameters of initial fracture, but deviation increases when the orientations of the initial flaw and of the external loading are more aligned. In all cases, the exact fracture path displays some variability and in particular for cases with a high degree of heterogeneity some caution must be used when interpreting fracture patterns from a limited number of samples. As a consequence deviation of damage patterns generated from numerical models when compared to experimental results can confidently be attributed to the particular realizations of the heterogeneity present in the real and numerical sample.
Factors affecting the reproducibility of fermentation of grape juice and of the aroma composition of wines 1. Grape maturity, sugar, inoculum concentration, aeration, juice turbidity and ergosterol
Creators: name: Houtman, A. C.
name: Marais, J.
name: Du Plessis, C. S.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2016

description: Gas chromatographic analysis was used for the investigation of some factors which determine the production of volatile wine components during fermentation. Especially the effect of "heterogenous" juice factors such as turbidity and air content were investigated in respect of fermentation rate and the production of fatty acids, fusel alcohols and esters. lt was shown that the fermentation lag of clear filtered and de-aerated grape juices could be prevented by small ergosterol additions before inoculation. A marked similarity was observed in the chromatograms of wines from fermentations of clear juices which were stimulated either by air or by ergosterol. Grape maturity proved to be a major factor affecting the production of volatile acids, alcohols and esters during fermentation. Fermentations of de-aerated and clear filtered juice with an addition of 0.5 g bentonite/l yielded wines with the best scores an sensory evaluation. Maßgebliche Faktoren für die Reproduzierbarkeit der Vergärung von Traubenmost und die Aromazusammensetzung von Wein I. Traubenreife, Zucker, Konzentration des lnokulums, Luft, Klarheit des Mostes und Ergosterin Einige Faktoren, welche die Bildung flüchtiger Weininhaltsstoffe bei der Gärung beeinflussen, wurden gaschromatographisch untersucht. Besonders die Wirkung „heterogener" Faktoren im Traubenmost, wie zum Beispiel Trübung und Luftgehalt, auf die Gärungsgeschwindigkeit und die Bildung von Fettsäuren, Fuselalkoholen und Estern wurde untersucht. Es wurde gezeigt, daß die Gärungsverzögerung bei klarfiltrierten und entlüfteten Traubensäften durch die Zufügung kleiner Mengen von Ergosterin vor der Inokulation verhindert werden kann. Wurde die Gärung von geklärtem Most durch Luft oder durch Ergosterin angeregt, so zeigten die Chromatogramme der Weine eine starke Ähnlichkeit. Die Traubenreife erwies sich als der wichtigste Faktor, der die Bildung von flüchtigen Säuren, Alkoholen und Estern bei der Gärung beeinflußt. Die Vergärung von entlüftetem und klarfiltriertem Most mit Zusatz von 0,5 g Bentonit/l ergab Weine, die bei der Weinprobe die höchsten Punktezahlen erhielten.
description: VITIS - Journal of Grapevine Research, Vol. 19 No. 1 (1980): Vitis
Purificazione per cromatografia di affinità dell' enzima malico estratto da uva
Creators: name: Spettoli, P.
name: Bottacin, A.
name: Zamorani, A.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2016

description: L'enzima malico (E.C. 1.1.1.40) estratto dall' uva Raboso è stato purificato per cromatografia di affinità. Sono state determinate le principali caratteristiche e le proprietà cinetiche dell' enzima prima e dopo il passaggio su colonna di affinità. L'enzima è NADP-specifico e la sua attività richiede cationi bivalenti. L'estratto prima della purificazione per cromatografia di affinità presenta valori di Km per NADP+ e per Mg2+ rispettivamente di. 0,035 mM e di 0,42 mM ed ha comportamento allosterico verso l' acido L-malico; dall' equazione di HILL si è trovato un mM di 1,86 ed il corrispondente valore di S0,5 uguale a 1,25 mM. Dopo la purificazione l' enzima non aveva più proprietà allosteriche e le Km sono state 0,066 mM per il NADP+, 0,30 mM per Mg2+ e 2 mM per l' acido L-malico. Purification of malic enzyme from grape berries by affinity chromatography Malic enzyme (E.C. 1.1.1.40) from Raboso grape berries has been purified by affinity chromatography. Main characteristics and kinetic properties before and after affinity chromatography column have been examined. The enzyme is NADP- specific and it requires divalent cations. Before purification, the enzyme has Km values for NADP+ and Mg2+ of 0.035 mM and 0.42 mM respectively; it shows allosteric behaviour to malate. The HILL plot slope for malate is 1.86 and S0,5 is 1.5 mM. The purified malic enzyme doesn't exhibit allosteric properties and Km is 0.066 mM for NADP+, 0.30 mM for Mg2+ and 2 mM for malate.
description: VITIS - Journal of Grapevine Research, Vol. 19 No. 1 (1980): Vitis
ESWC Workshop report: Empirical 2014
Creators: name: Kjernsmo, Kjetil
name: Maria-Esther Vidal
name: Ossenbruggen, Jacco Van
Publisher: name: figshare
Year: 2014
Subjects: subject: Library and Information Studies
subject: FOS: Media and communications
subject: Information Systems
subject: FOS: Computer and information sciences
subject: Science Policy
subject: Applied Computer Science

description: http://2014.empirical-ws.org/
Providing Financial Services “Efficiently, Honestly and Fairly”: Part 2
Creators: name: Latimer, Paul
Publisher: name: Swinburne
Year: 2024
Subjects: classificationCode: 480102
subject: Commercial law

description: This article is written to update the author’s earlier article published in this journal in 2006 which examined the statutory obligation of a financial services licensee to act “efficiently, honestly and fairly” as required by s 912A(1)(a) of the Corporations Act 2001 (Cth). The article examines new caselaw since 2006 on the meaning of this obligation. The weight of authority supports interpreting the conjunction “and” to confirm that this is a single, composite and omnibus obligation rather than three separate obligations. Further, the failure of those in the financial services industry to act efficiently, honestly and fairly was cited repeatedly in the context of the evidence of widespread misconduct falling below community expectations revealed by the Royal Commission into Misconduct in the Banking, Superannuation and Financial Services Industry (2018/2019). Some of the limits of enforcing this law are raised, and the article concludes with an introduction to the new civil penalties in financial services regulation introduced in the Treasury Laws Amendment (Strengthening Corporate and Financial Sector Penalties) Act 2019 (Cth). These have strengthened penalties for breaches of corporate laws including this section and should enhance and will support ASIC’s new “why not litigate” approach. The Royal Commission was clear that acting efficiently, honestly and fairly is part of the "right” thing to do.
Speculation
Creators: name: Gare, Arran
Publisher: name: Swinburne
Year: 2024

description: “Speculation” originally meant “reflective observation.” It came to mean “conjecture” or “mere conjecture” as philosophers strove for certainty, consecrating science as rigorously acquired knowledge accumulated through application of the scientific method, and devalued the cognitive status of other discourses. The present conventional meaning of speculation, where the place of observation has disappeared, is a by-product of this consecration. In this entry I show how, through efforts to defend the status of these other discourses, the original meaning of “speculation” was not only revived but built upon by speculative philosophers. They showed that speculation is primordial to all experience and thinking, with past speculations embodied in language as “dead” metaphors. Revealing the possibility of elaborating alternative metaphors frees us from not only these dead metaphors to overcome the dead ends of current science, opening up new possibilities for enquiry, but the possibility of reconceiving ourselves and our place in nature. In this way, speculation makes it possible to transform ourselves, creating radically new ways of living and new forms of life. On this view, speculation, by opening new possibilities, could free us from the destructive trajectories of current civilization.
Proton reduction at surface of transition metal nanocatalysts
Creators: name: Qingguo Meng
name: Jiangchao Chen
name: Kilin, Dmitri
Publisher: name: Taylor & Francis
Year: 2014
Subjects: subject: Science Policy
subject: Biological Sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: Chemistry

description: Catalytic activities of neutral and charged palladium (Pd) nanoparticles are compared for hydrogen reduction half-reaction. In this work the sequential H2 dissociation from the surface of a Pd13H24 cluster is systematically studied by ab initio molecular dynamics (AIMD) at the density functional theory level. AIMD simulation is launched by preparing initial values of momenta of all nuclei in the model corresponding to a temperature range of 0–1700 K. AIMD simulation provides the trajectories of all the atoms in the cluster. A sequential H2 desorption up to seven molecules is observed from the cluster surface due to thermal motion of nuclei. Modifications of total charge on the neutral Pd13H24 cluster model are found to affect surface H2 desorption behaviour. A desorption rate of H2 molecule on both neutral and charged Pd13H24 clusters is compared to the data of Pt13H24 cluster reported previously. The H2 desorption energy on all the investigated clusters is also determined. The results reveal that Pd13 cluster presents a higher catalytic activity than Pt13 cluster.
Comparação da densidade e gordura corporal de cardiopatas entre grupos de 40 e 49 anos e acima de 50 anos de idade
Creators: name: Jônatas De França Barros
name: Neto, Cândido Simões Pires
name: Arbelo, Clarice
name: Aluisio Otavio Vargas Avila
Publisher: name: Zenodo
Year: 1992

description: Trabalho apresentado em Congresso
Technical Fixes as Challenges to State Legitimacy: Australian Separated Fathers’ Suggestions for Child Support Policy Reform
Creators: name: Cook, Kay
name: Skinner, Christine
Publisher: name: Swinburne
Year: 2024

description: This article assesses fathers' evidence presented to an Australian inquiry into the child support scheme. We examine these data in order to address how fathers' proposed child support policy solutions compared against Eekelaar's critique of parents' moral responsibilities to children and his identification of three substitute social bases for parents' continued support. We find that despite the inquiry's technical remit, fathers' solutions challenged the very basis of child support as maintaining, reinforcing, or redressing their responsibilities to children. Here, we illustrate that such procedures may be unable to contain fundamental challenges to state legitimacy when dealing with contested social issues.
Comparação de espessura das dobras cutâneas subescapular, tricipital, bicipital e supra-ilíaca de sujeitos cardiopatas e não cardiopatas
Creators: name: Jônatas De França Barros
name: Neto, Cândido Simões Pires
name: Neto, Clarice Arbelos
name: Aluísio Otávio Vargas Avila
Publisher: name: Zenodo
Year: 1992

description: Trabalho apresentado em Congresso.
Performance of Thermally Insulated Reinforced Concrete Beams Strengthened with Near-Surface Mounted-Carbon Fiber Reinforced Polymer Strips under Elevated Temperature
Creators: name: Saeed, Ikram Ameen
name: Al-Attar, Tareq S.
name: Al-Shathr, Basil S.
name: Al-Mahaidi, Riadh
Publisher: name: Swinburne
Year: 2024

description: This paper investigated the performance of reinforced concrete beams strengthened with near-surface mounted (NSM) carbon fiber reinforced polymer (CFRP) smooth and rough strips using cement based adhesive and insulated with plasterboards for protection the beams subjected to high temperature. An experimental work was carried out on ten large scale RC strengthened beams and strengthened insulated beams. The experimental program subject the strengthened beams to high temperature degrees up to 600 °C in electric furnace and observe changes after beams been heated. Results showed that the plasterboards provided excellent protection and decreased the CFRP temperature of about 42-24%. The insulated beams behaved as good as that of strengthened beams prior to expose to 600°C.
ROB Seminar Presentation - Demystifying AI: The Past, Present and Future
Creators: name: Bhattacharya, Aditya
Publisher: name: Zenodo
Year: 2024

description: In this beginner-friendly seminar, we will explore the transformative journey of artificial intelligence (AI)and machine learning (ML), contrasting their innovative approaches with traditional software engineering.Attendees will gain insights into data-driven decision-making and real-world applications of AI/ML, highlighting theirprofound impact across various domains. The seminar will also cover the latest developments in Generative AI,including the latest breakthroughs in image, audio, and video generation. Emphasising the importance oftransparency, we will discuss the emerging field of Explainable and Responsible AI, addressing ethicalconsiderations and societal impacts. As we look ahead, we will speculate on how AI will shape our future,culminating with a forward-looking vision of AI by 2030. Join us for an engaging discussion on the evolution, currentstate, and future trajectory of AI.
Annotation of the Human Genome by High-Throughput Sequence Analysis of Naturally Occurring Proteins
Creators: name: Southan, Christopher
Publisher: name: figshare
Year: 2014
Subjects: subject: Biotechnology
subject: 60102 Bioinformatics
subject: FOS: Computer and information sciences

description: (n.b. figshare bug prevents me adding OGS Team as first authors. You could cite with Bentham doi from link but author list was truncated anyway)

 Abstract: The identification of protein-coding genes is currently based on the merging of evidence and predictions from a variety of databases that may themselves contain inaccurate and partial information. We have developed a method for mapping accurate interpretations of protein MS-MS data to the genome. This approach enables verification of genes, exons, transcripts and variant transcripts as well as the de novo discovery of novel protein-coding genes. Here we describe improvements in spectral interpretation algorithms, multiple separation techniques, sub-cellular fractionation and novel bioinformatics approaches to characterise more than 14,000 naturally occurring human genes.
Coal seam gas distribution and hydrodynamics of the Sydney Basin, NSW, Australia
Creators: name: A. Burra
name: J. S. Esterle
name: S. D. Golding
Publisher: name: Taylor & Francis
Year: 2014
Subjects: Uncategorized

description: This paper reviews various coal seam gas (CSG) models that have been developed for the Sydney Basin, and provides an alternative interpretation for gas composition layering and deep-seated CO2 origins. Open file CSG wells, supplemented by mine-scale information, were used to examine trends in gas content and composition at locations from the margin to the centre of the basin. Regionally available hydrochemistry data and interpretations of hydrodynamics were incorporated with conventional petroleum well data on porosity and permeability. The synthesised gas and groundwater model presented in this paper suggests that meteoric water flow under hydrostatic pressure transports methanogenic consortia into the subsurface and that water chemistry evolves during migration from calcium-rich freshwaters in inland recharge areas towards sodium-rich brackish water down-gradient and with depth. Groundwater chemistry changes result in the dissolution and precipitation of minerals as well as affecting the behaviour of dissolved gases such as CO2. Mixing of carbonate-rich waters with waters of significantly different chemistries at depth causes the liberation of CO2 gas from the solution that is adsorbed into the coal matrix in hydrodynamically closed terrains. In more open systems, excess CO2 in the groundwater (carried as bicarbonate) may lead to precipitation of calcite in the host strata. As a result, areas in the central and eastern parts of the basin do not host spatially extensive CO2 gas accumulations but experience more widespread calcite mineralisation, with gas compositions dominated by hydrocarbons, including wet gases. Basin boundary areas (commonly topographic and/or structural highs) in the northern, western and southern parts of the basin commonly contain CO2-rich gases at depth. This deep-seated CO2-rich gas is generally thought to derive from local to continental scale magmatic intrusions, but could also be the product of carbonate dissolution or acetate fermentation.
Gel Electrophoresis
Creators: name: Garg, HK
Publisher: name: Zenodo
Year: 2022
Subjects: Gel Electrophoresis
Agarose Gel Electrophoresis
Nylon Membrane
DNA Separation
Charge Mass Ratio
Electric Field

description: Gel electrophoresis is used to separate and analyze biomolecules, such as DNA, RNA, and proteins. By applying an electric field to a gel matrix, charged molecules migrate at varying speeds based on their size and charge. This process enables researchers to visualize and quantify nucleic acids and proteins, making it an essential tool for genetic research, forensic analysis, and medical diagnostics.

Its reliability, efficiency, and capacity for high-throughput analysis make it indispensable for scientists seeking to unlock the obscurities of the molecular world.
Coal seam gas distribution and hydrodynamics of the Sydney Basin, NSW, Australia
Creators: name: A. Burra
name: J. S. Esterle
name: S. D. Golding
Publisher: name: Taylor & Francis
Year: 2014
Subjects: Uncategorised

description: This paper reviews various coal seam gas (CSG) models that have been developed for the Sydney Basin, and provides an alternative interpretation for gas composition layering and deep-seated CO2 origins. Open file CSG wells, supplemented by mine-scale information, were used to examine trends in gas content and composition at locations from the margin to the centre of the basin. Regionally available hydrochemistry data and interpretations of hydrodynamics were incorporated with conventional petroleum well data on porosity and permeability. The synthesised gas and groundwater model presented in this paper suggests that meteoric water flow under hydrostatic pressure transports methanogenic consortia into the subsurface and that water chemistry evolves during migration from calcium-rich freshwaters in inland recharge areas towards sodium-rich brackish water down-gradient and with depth. Groundwater chemistry changes result in the dissolution and precipitation of minerals as well as affecting the behaviour of dissolved gases such as CO2. Mixing of carbonate-rich waters with waters of significantly different chemistries at depth causes the liberation of CO2 gas from the solution that is adsorbed into the coal matrix in hydrodynamically closed terrains. In more open systems, excess CO2 in the groundwater (carried as bicarbonate) may lead to precipitation of calcite in the host strata. As a result, areas in the central and eastern parts of the basin do not host spatially extensive CO2 gas accumulations but experience more widespread calcite mineralisation, with gas compositions dominated by hydrocarbons, including wet gases. Basin boundary areas (commonly topographic and/or structural highs) in the northern, western and southern parts of the basin commonly contain CO2-rich gases at depth. This deep-seated CO2-rich gas is generally thought to derive from local to continental scale magmatic intrusions, but could also be the product of carbonate dissolution or acetate fermentation.
Oxime-dipeptides as anticholinesterase, reactivator of phosphonylated-serine of AChE catalytic triad: probing the mechanistic insight by MM-GBSA, dynamics simulations and DFT analysis
Creators: name: Chadha, Nidhi
name: Tiwari, Anjani K.
name: Kumar, Vikas
name: Lal, Sangeeta
name: Milton, Marilyn D.
name: Mishra, Anil K.
Publisher: name: Taylor & Francis
Year: 2015
Subjects: Information and Computing Sciences
Biological Sciences
Chemistry
Biochemistry
Biophysics
Molecular Biology

description: Neuropathological cascades leading to reduced cholinergic transmission in Alzheimer’s disease led to development of AChE-inhibitors. Although lethal dose of some inhibitors cause interruption with AChE mediated mechanism but reversible AChE inhibitors can assist in protection from inhibition of AChE and hence in an aim to probe potential molecules as anticholinesterase and as reactivators, computationally structure-based approach has been exploited in this work for designing new 2-amino-3-pyridoixime-dipeptides conjugates. We have combined MD simulations with flexible ligand docking approach to determine binding specificity of 2-amino-3-pyridoixime dipeptides towards AChE (PDB 2WHP). PAS residues are found to be responsible for oxime-dipeptides binding along with π–π interactions with Trp86 and Tyr286, hydrogen bonding with side chains of Asp74 and Tyr341 (Gscore –10.801 and MM-GBSA free energy –34.89 kcal/mol). The docking results depicted complementary multivalent interactions along with good binding affinity as predicted from MM-GBSA analysis. The 2-amino-3-pyridoxime-(Arg-Asn) AChE systems subjected to MD simulations under explicit solvent systems with NPT and NVT ensemble. MD simulations uncovered dynamic behavior of 2-amino-3-pyridoxime-(Arg-Asn) and exposed its mobile nature and competence to form strong long range-order contacts towards active site residues to approach inhibited serine residue and facilitated via large contribution from hydrogen bonding and water bridges along with slow and large movements of adjacent important residues. In an effort to evaluate the complete potential surface profile, 2-amino-3-pyridoxime induced reactivation pathway of sarin–serine adduct has been investigated by the DFT approach at the vacuum MO6/6–311G (d, p) level along with the Poisson-Boltzmann solvation model and found to be of relatively low energy barrier. The pKa evaluation has revealed the major deprotonated 2-amino-3-pyridoixime species having pKa of 6.47 and hence making 2-amino-3-pyridoxime-(Arg-Asn) potential anticholinesterase and reactivator for AChE under the physiological pH.
title: Characterization of β-Lactam Resistant Pandemic Serotypes of Vibrio cholerae Isolated from Ships' Ballast Tanks and Coastal Waters
Creators: name: Goodrich, Amanda Lynn
Publisher: name: Old Dominion University
publisherIdentifier: https://ror.org/04zjtrb98
publisherIdentifierScheme: ROR
Year: 2006

description: Vibrio cholerae serotypes 01 and 0139 are responsible for world-wide epidemics of cholera. These pandemic causing strains must possess genes that encode for the cholera toxin (CTX) and toxin co-regulated pili (TCP) in order to infect their hosts. In this study, 284 isolates of Vibrio cholerae from ballast and coastal waters were serotyped, with 11 % testing positive for serotype 01 and 21 % testing positive for serotype 0139. PCR assays were used to detect the presence of ctxA and tcpA genes in all positive isolates, none of which contained ctxA while 2% of the isolates contained tcpA. The 01 and 0139 isolates were tested using a nitrocefin assay for the presence of 13-lactamase, an enzyme that protects V cholerae from 13-lactam antibiotics. These results were combined with antibiotic-susceptibility profiles and the isolates were divided into 4 different groups defined by their antibiotic characteristics. Finally, the plasmids of 3 isolates were digested using restriction enzymes and compared according to the band sizes produced.

Fifty percent of isolates from ballast tanks were found to be serotype 0139. This result is consistent with current pandemics of cholera which are considered to be caused by this serotype. It is also thought to be the more virulent and robust of the two pandemic serotypes. The dearth of ctxA and tcpA gene sequences, however, is expected in environmental isolates from areas not undergoing a cholera pandemic. Among the isolates collected from coastal areas, those that were serotype 01 were most often found in very near-shore environments while serotype 0139 were most often collected from open water environments, a difference possibly related to their methods of attachment to plankton and other particles. Serotype 0139 exhibited less antibiotic-resistance and was less likely to produce P-lactamase than serotype 01. This result supports data from other studies which determined that serotype 01 is more resistant to P-lactam antibiotics while serotype 0139 is more sensitive to them.

Ballast water management is important for preventing the spread of Vibrio cholerae and possible resulting pandemics. The International Maritime Organization has established some regulations on ballast water but more consideration needs to be given towards bacteria in other ballast tank environments, such as biofilms and sediment, as well as non-01 and non-0139 serotypes of Vibrio cholerae.
Development and Characterization of Soy Lecithin and Palm Oil-based Organogels
Creators: name: Nirod Baran
name: Singh, Vinay K.
name: Kunal Pal
name: Arfat Anis
name: Dillip K. Pradhan
name: Pramanik, Krishna
Publisher: name: Taylor & Francis
Year: 2014
Subjects: Uncategorized

description: Preparation and characterization of soy lecithin (SL) and palm oil (PO) based organogels have been reported in this study. The optimization of the composition of the organogels was carried out by varying the proportions of SL, PO and water. Microscopic studies suggested the presence of aqueous phase either as spherical droplets or fluid filled fibers or both, depending on the composition of the organogels. FTIR study indicated strong intermolecular hydrogen bonding among the organogel components. The release of metronidazole (model drug, MZ) suggested diffusion mediated drug release. MZ loaded organogels showed good antimicrobial property against B. subtilis and E. coli.
Development and Characterization of Soy Lecithin and Palm Oil-based Organogels
Creators: name: Nirod Baran
name: Singh, Vinay K.
name: Kunal Pal
name: Arfat Anis
name: Dillip K. Pradhan
name: Pramanik, Krishna
Publisher: name: Taylor & Francis
Year: 2014
Subjects: Uncategorised

description: Preparation and characterization of soy lecithin (SL) and palm oil (PO) based organogels have been reported in this study. The optimization of the composition of the organogels was carried out by varying the proportions of SL, PO and water. Microscopic studies suggested the presence of aqueous phase either as spherical droplets or fluid filled fibers or both, depending on the composition of the organogels. FTIR study indicated strong intermolecular hydrogen bonding among the organogel components. The release of metronidazole (model drug, MZ) suggested diffusion mediated drug release. MZ loaded organogels showed good antimicrobial property against B. subtilis and E. coli.
title: Spatial Analyses and Repletion of Gargathy Coastal Lagoon
Creators: name: Gomez, Loreto Herraiz
Publisher: name: Old Dominion University
publisherIdentifier: https://ror.org/04zjtrb98
publisherIdentifierScheme: ROR
Year: 2008

description: Coastal lagoons and bays vary in shape and size in response to antecedent topography, geologic processes and sea level rise. Variations in shape and environmental conditions of coastal basins are believed to influence the distribution of benthic sub-environments and the exchange of water with the ocean and other adjacent coastal systems. Gargathy Inlet and its coastal lagoon vary spatially from the inlet, where the greatest depths are observed, to the mainland, dominated by shallow intertidal areas, colonized by marsh. Hypsographic and hydro-hypsographic analyses of Gargathy's coastal lagoon were the primary techniques applied to understand the relative distribution of the benthic and pelagic surface areas at different elevations. Using these techniques, four benthic sub-environments, upper intertidal benthic environment, lower intertidal flats, shallow sub-tidal lagoons and sub-tidal channels were differentiated. The Hypsographic curve of Gargathy Lagoon illustrated that 80.81 % of the total surface area is colonized by marsh; however, only 27% of the basin volume occurs over the marsh.

Water exchange and flushing characteristics of this marsh-dominated system were associated with volume-hypsography, the hydraulic tum-over time and the repletion footprint. The volume hypsographic curve for Gargathy lagoon, illustrated that about 77.3% of the basin capacity is completely exchanged with coastal oceanic waters with each tidal cycle. If repletion water completely mixed with residual water between tidal cycles, then, hydraulic tum-over time (HTT) could be as low as 1.29 tidal cycles. Mixing between residual water and repletion water is strongly influenced by hypsometry. Repletion patterns are strongly influenced by the presence of marsh during the last stages of the tidal cycle when water spreads over the marsh surfaces after filling the tidal channels. This study provides spatial techniques and methods that allow for a better understanding and characterization of coastal basins.
title: Feeding Morphologies and Distribution Patterns of Marine Cladocera in the Lower Chesapeake Bay
Creators: name: Gilchrist, Sandra Layne
Publisher: name: Old Dominion University
publisherIdentifier: https://ror.org/04zjtrb98
publisherIdentifierScheme: ROR
Year: 1979

description: Temporal and spatial distributions of marine cladocera in the lower Chesapeake Bay estuary were observed over a year-long sampling period. The distributions of cladoceran species throughout the Bay were studied in relation to temperature and salinity. A survey of the species over the entire Bay is included to establish distribution patterns of the various species during a short time period.

Theoretical mathematical principles were used to predict feeding abilities of the Bay species. Mouthparts are described qualitatively and quantitatively to infer function. A mandibular index developed by Itoh (1970) was revised and employed in evaluating feeding methods interspecifically and interspecifically.

Illustrations of primary and secondary feeding structures were made and used for taxonomic verification of the species. Discrepancies in keys prepared for geographically isolated populations by workers in various parts of the United States, Russia, and South America necessitated a re-evaluation of fundamental taxonomic characteristics to include modifications for Bay species noted during the course of the present study.
Die Geschichte des ‚Rauhen Kilikien‘ unter den Römern
Creators: name: Pilhofer, Philipp
Publisher: name: Humboldt-Universität zu Berlin
Year: 2020
Subjects: subject: Rauhes Kilikien
subject: Kalykadnos
subject: Isaurien
subject: Antiochos IV.
subject: Piraten
subject: 930 Geschichte des Altertums bis ca. 499

description: In dieser landeskundlich-historisch ausgerichteten Abhandlung wird die Geschichte des ‚Rauhen Kilikien‘ unter den Römern untersucht. Das Rauhe Kilikien in seiner Gesamtheit stand bislang nur selten im Zentrum einer genaueren Analyse. Die geschichtlichen Daten sind möglichst quellennah unter Berücksichtigung der Sekundärliteratur erarbeitet; insbesondere wurde dabei der epigraphische Befund berücksichtigt. Einer einleitenden geographischen Abgrenzung folgt eine kurze Einführung in die Region. Das Rauhe Kilikien wurde länger als alle anderen kleinasiatischen Regionen von Klientelkönigen verwaltet, bis es unter Vespasian Teil der römischen Provinzordnung wurde. Anschließend erlebte das Rauhe Kilikien eine lange Phase der steten Entwicklung und Urbanisierung. Unter Diokletian wurde das Rauhe Kilikien Kernstück der neuen Provinz ‚Isauria‘. Ein besonderer Schwerpunkt der Untersuchung liegt auf der Frage nach den ‚kilikischen Piraten‘ und, später, den ‚isaurischen Banditen‘.
Change in affective well-being on change in perceived job characteristics: The mediating role of hope
Creators: name: Reis, Dorota
name: Hoppe, Annekatrin
Publisher: name: Humboldt-Universität zu Berlin
Year: 2015
Subjects: subject: 150 Psychologie

description: Research on occupational health has consistently shown that job characteristics and personal resources predict employee well-being. Building on the associative network theory, we claim that – vice versa – well-being is likely to affect the perception of job characteristics and personal resources. The aim of this study was to expand the literature on job characteristics, personal resources, and employee well-being (1) by taking a reversed causation perspective and (2) by investigating the dynamic nature of these relations in a latent change model. More specifically, we hypothesized that baseline levels and change in affective well-being are related to change in emotional demands and autonomy, two core job characteristics for our sample of psychotherapists. In addition, we explored the mediating role of hope as a personal resource in this process. A total of 326 psychotherapists participated in a two-wave online survey with a 5-month time lag. Results revealed that baseline levels of and change in affective well-being were associated with change in emotional demands. Furthermore, change in hope mediated the effect of change in affective well-being on change in autonomy. In conclusion, the results show that affective well-being can mark a starting point for building personal resources and changing employees' perceptions of their job characteristics.
title: Prediction of host-pathogen gene expression from dual RNA-seq data during a bacterial infection
Creators: name: Barani Lonbani, Nima
Publisher: name: Memorial University of Newfoundland
publisherIdentifier: https://ror.org/04haebc03
publisherIdentifierScheme: ROR
Year: 2024

description: Understanding the mechanisms by which bacteria cause disease, such as apoptosis and inflammatory signals, necessitates a comprehensive knowledge of the genes expressed during infection by both the host and the pathogen. Dual RNA-seq technology enables simultaneous detection of transcripts of the pathogen and host during an infection. In this study, we utilized machine learning to predict the expression levels of genes involved in bacterial infection from their RNA sequence using dual RNA-seq data to obtain gene expression levels. We developed two predictive models: one specifically tailored to the host and the other to the pathogen. Results from these models are promising in terms of macro-average F1-score and macro-average Area Under Receiver Operating Characteristic Curve (AUROC) and demonstrate that machine learning can be applied to dual RNA-seq data to predict gene expression levels during bacterial infection, opening new prospects for future research to build upon these methods and insights.
A transient phreatic surface mound, evidenced by a strip of vegetation on an earth dam
Creators: name: A.R. Kacimov
name: G. Brown
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Cancer
subject: Biological Sciences
subject: Ecology
subject: FOS: Biological sciences
subject: Earth and Environmental Sciences
subject: Evolutionary Biology

description: On the slopes of the embankment of the Al-Khod groundwater recharge–flood protection dam (Oman), a band of scrub vegetation community emerged after torrential rains and temporary filling of the dam reservoir. Species composition differs markedly on both sides of the embankment, with many exotics found on the reservoir side and more typical gravel-desert species on the outside. Hydro-ecologically, the vegetation is interpreted as the footprint of a temporary storage of water, which is a small-sized groundwater mound within the permeable shoulder of the levee. The levee, as an anthropogenic landform, induces a U-turn (gravitational slumping–lateral seepage–transpirational moisture ascent) topology of seepage. The Lembke method of successive variations of steady states is used in modelling the water table dynamics. In the early stage of the mound decay, outflow through a seepage face of the shoulder is modelled by the Barenblatt slumping parabola of the phreatic-zone part of the flow domain, which is perfectly matched with the Youngs exact solution for a purely horizontal flow through a porous wedge. At the stitching cross-section, the flow rates and saturated depths in the two zones coincide. The late stage of mound evolution is characterized by transpiration by the plant roots projected onto a shrinking free surface, with the Barenblatt and Youngs solutions conjugated but without the outcrop of the saturated mound on the levee slope. Ordinary differential equations for the sliding or descending locus of the intersection of the parabola and the triangle hypotenuse are integrated in a closed form or by the Runge-Kutta method. The dwindling saturated volume and the rate of drainage are obtained. They can be used in assessments of the hydro-ecological sustainability of slope-rooted shrubs (vegetation survival between rare rainfall episodes).Editor D. Koutsoyiannis; Associate editor A. Porporato
Towards a business and production engineering concept for individual beer brewing applying digitalization methodologies
Creators: name: Schlechter, T.
name: Froschauer, R.
name: Bronowicka-Schlechter, A.
Publisher: name: Agronomy Research
Year: 2020
Subjects: digital factory
individual food production
smart manufacturing
gamification
industry 4.0.
article

description: Individualization is a common trend in many fields of production across the industries. Also in the food sector, significant changes can be observed. For many products, individual offerings towards the customer are meanwhile either mandatory or at least help to increase the sales and revenue. Somehow, individual product design and production contradicts scaling effects, which are especially important for food production. On the other hand, as digitalization is implemented in a fairly limited way in the food sector, currently great chances can be observed to build a unique selling proposition and consequently gain market share by implementing appropriate measures to enable a digital food factory. This is where the proposed idea comes into the game. The starting point is the idea to produce individually developed beer and ship it to the individual customer. The beer can be designed on a web page based on typical parameters, like beer type, bitterness, colour, or alcohol concentration. In an expert mode, individual beer creations may be thoughtful, allowing the creation of completely individual recipes (for sure, not guaranteeing the customer a perfect drinking experience). In any way, the data from the web page is directly fed to the brewing equipment in the brewing facility. There, using newly to be developed specialized machines, the individually ordered beer will be produced automatically. In this paper we discuss the individual challenges at each point in the production cycles and propose solutions to those.
Rapid purification of grapevine RNA by a simple electrophoretic method
Creators: name: Szegedi, E.
name: Otten, L.
name: Váradi, Gy.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Research Note
description: VITIS - Journal of Grapevine Research, Vol. 33 No. 3 (1994): Vitis
Copper(II) directs formation of toxic amorphous aggregates resulting in inhibition of hen egg white lysozyme fibrillation under alkaline salt-mediated conditions
Creators: name: Ghosh, Sudeshna
name: Nitin K. Pandey
name: Banerjee, Priyanka
name: Koel Chaudhury
name: Nagy, Nóra Veronika
name: Swagata Dasgupta
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Biological Sciences
subject: Ecology
subject: FOS: Biological sciences
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Neuroscience
subject: Physics
subject: Biochemistry
subject: Biophysics

description: Hen egg white lysozyme (HEWL) adopts a molten globule-like state at high pH (~12.75) and is found to form amyloid fibrils at alkaline pH. Here, we report that Cu(II) inhibits self-association of HEWL at pH 12.75 both at 37 and 65 °C. A significant reduction in Thioflavin T fluorescence intensity, attenuation in β-sheet content and reduction in hydrophobic exposure were observed with increasing Cu(II) stoichiometry. Electron paramagnetic resonance spectroscopy suggests a 4N type of coordination pattern around Cu(II) during fibrillation. Cu(II) is also capable of altering the cytotoxicity of the proteinaceous aggregates. Fibrillar species of diverse morphology were found in the absence of Cu(II) with the generation of amorphous aggregates in the presence of Cu(II), which are more toxic compared to the fibrils alone.
Regeneration of grapevine (Vitis sp.) protoplasts
Creators: name: Reustle, G.
name: Harst, Margit
name: Alleweldt, G.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Research Note
description: VITIS - Journal of Grapevine Research, Vol. 33 No. 3 (1994): Vitis
Effect of nitrogen and potassium fertilizer on induced resveratrol synthesis in two grapevine genotypes
Creators: name: Bavaresco, E.
name: Fregoni, M.
name: Petegolli, D.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Research Note One-year-old grapevine cuttings of the interspecific hybrid Castor and V. vinifera L. cv. Bacchus were grown in pots with 4 combined doses of nitrogen and potassium, in order to test their effect on the resveratrol synthesis of the leaves, after elicitation with mucic acid (0.01 %). The tests were performed on leaf discs, inside bio-assay dishes. The most significant findings were: • the fertilizer treatments had a higher effect on the resveratrol synthesis of Castor than in Bacchus; • high potassium supply together with low nitrogen rate promoted the resveratrol synthesis of Castor; • high potasium rates did not balance the negative effect of high nitrogen supply on resveratrol synthesis.
description: VITIS - Journal of Grapevine Research, Vol. 33 No. 3 (1994): Vitis
Inhibition of fungal infection using sulfite pads prior to initiation of callus from Vitis labruscana cv. Concord
Creators: name: Shure, K. B.
name: Henick-Kling, T.
name: Acree, T. E.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Research Note Incubation of plant material with potassium metabisulfite was found to inhibit fungal infections of explants from grapevines. Grapevine tissue of Vitis labruscana cv. Concord was incubated with sulfite pads containing 0.4 g of potassium metabilsufite for one and two days prior to culturing and evaluated against a control that had been surface sterilized with 0.5 % NaOCI and 70 % ethanol after one week for losses due to microbial contamination. Sulfite fumigation of plant material reduced the incidence of mold infection, particularly in tissue cultures developed from fruit explants which had reductions in contamination as high as 10 fold. Continued attempts to isolate contaminants from cultures intitiated from these explants showed no signs of infection.
description: VITIS - Journal of Grapevine Research, Vol. 33 No. 3 (1994): Vitis