cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Modelling of top submerged swirl and non-swirl gas injections into liquid baths
Creators: name: Yang, Wei
Publisher: name: Swinburne
Year: 2024

description: Experimental and numerical investigations were carried out on the fluid flow behaviour within cylindrical liquid baths agitated by a top submerged gas injection lance system. The effects of key hydrodynamic parameters, such as the swirl and non-swirl gas injection, the gas injection flow rate, the level of submergence and the depth and size of liquid bath, on the flow structure and the mixing process within the bath were investigated. Experimental and numerical results from the current study enabled to gain more knowledge and physical understanding of such system, and to determine the optimum operating conditions for the industrial systems. The Laser Doppler Anemometry (LDA) measurement technique was employed to determine the mean and turbulent flow structure in laboratory models of top submerged gas injection into liquid baths. Two cylindrical air-water models were constructed according to the geometric similarity criteria related to the real industrial smelting furnaces. The experimental operating conditions were then decided based on the Froude number dynamic similarity criterion. The three-dimensional flowfield structures of the liquid phase including the time mean velocity components and turbulence parameters were obtained with the aid of LDA under forty-two combinations of the operating conditions for both laboratory models. A digital video recording technique was also employed to study the plume characteristics due to the LDA limitation in obtaining the velocities in the plume region. Numerical simulations of the fluid flow structure within a liquid bath that is agitated by a top submerged gas injection system were carried out using the commercial Computational Fluid Dynamics (CFD) software package CFX4. A two-dimension gas-liquid transient numerical model was developed by using the Eulerian-Eulerian approach. The standard k-Ε model was employed to predict the turbulence structure of the liquid phase. The comparison of the numerical predictions with experimental data under various operating conditions showed a reasonable agreement on both mean and turbulent fluid flow characteristics.
Efficient Computation of the Long-Range Exact Exchange using an Extended Screening Function
Creators: name: Kokott, Sebastian
name: Blum, Volker
name: Scheffler, Matthias
Publisher: name: arXiv
Year: 2025
Subjects: subject: Materials Science (cond-mat.mtrl-sci)
subject: Chemical Physics (physics.chem-ph)
subject: FOS: Physical sciences

description: We introduce a computationally efficient screening for the Coulomb potential that also allows calculating approximated long-range exact exchange contributions with an accuracy similar to an explicit full-range evaluation of the exact exchange. Starting from the screening function of the HSE functional, i.e., the complementary error function, as zeroth order, a first-order Taylor expansion in terms of the screening parameter ω is proposed as an approximation of the long-range Coulomb potential. The resulting extended screening function has a similar spatial extend as the complementary error function leading to a computational speed comparable to screened hybrid functionals such as HSE06, but with long-range exact exchange contributions included. The approach is tested and demonstrated for prototypical semiconductors and organic crystals using the PBE0 functional. Predicted energy band gaps, total energies, cohesive energies, and lattice energies from the first-order approximated PBE0 functional are close to those from the unmodified PBE0 functional, but are obtained at significantly reduced computational cost.
The Behaviour of Plank (Tongue and Groove) Wood Decking Under the Effects of Uniformly Distributed and Concentrated Loads
Creators: name: Rocchi, Kevin
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2013
Subjects: Plank (Tongue and Groove) Wood Decking
Uniformly Distributed Loads
Concentrated Loads

description: Plank (tongue and groove) wood decking is a product that is commonly used in post and beam timber construction to transfer gravity loads on roofs and floors. In 2010, The National Building Code of Canada changed the application area of the specified concentrated roof live loads from 750 mm x 750 mm to 200 mm x 200 mm. The change was made to better reflect the area which a construction worker with equipment occupies. Preliminary analysis showed that the change in the application area of concentrated loads may have a significant impact on the design of decking systems. Little research or development has been done on plank decking since the 1950’s and 1960’s. An experimental program was undertaken at the University of Ottawa’s structural laboratory to better understand the behaviour of plank decking under uniformly distributed and concentrated loads. Non-destructive and destructive tests were conducted on plank decking systems to investigate their stiffness and failure mode characteristics under uniformly distributed as well as concentrated loads. The experimental test program was complimented with a detailed finite element model in order to predict the behaviour of a plank decking system, especially the force transfer between decks through the tongue and groove joint. The study showed that the published deflection coefficients for uniformly distributed loads can accurately predict the three types of decking layup patterns specified in the Canadian Design Standard (CSA O86, 2009). For unbalanced uniformly distributed loads on two-span continuous layup, it was found that the deflection coefficient of 0.42 was non-conservative. It was also found that under concentrated loads, the stiffness of the decking system increased significantly as more boards were added. A deflection coefficient of 0.40 is appropriate to calculate the deflection for the three types of decking layup patterns specified in the Canadian Design Standard (CSA O86, 2009) under concentrated load on an area of 200 mm by 200 mm. Significant load sharing was observed for plank decking under concentrated loads. An increase in capacity of about 1.5 to 2.5 times the capacity of the loaded boards was found. Furthermore, it was found that placing sheathing on top of a decking system had a significant effect in the case of concentrated load with an increase of over 50% in stiffness and over 100% in ultimate capacity.
"Salt is the Soul of Hakka Baked Chicken": Reimagining Traditional Chinese Culinary ICH for Modern Contexts Without Losing Tradition
Creators: name: Liu, Sijia
name: Zeng, XiaoKe
name: Wu, Fengyihan
name: Ye, Shu
name: Liu, Bowen
name: Cheung, Sidney
name: Allen, Richard William
name: Lc, Ray
Publisher: name: arXiv
Year: 2025
Subjects: subject: Human-Computer Interaction (cs.HC)
subject: FOS: Computer and information sciences

description: Intangible Cultural Heritage (ICH) like traditional culinary practices face increasing pressure to adapt to globalization while maintaining their cultural authenticity. Centuries-old traditions in Chinese cuisine are subject to rapid changes for adaptation to contemporary tastes and dietary preferences. The preservation of these cultural practices requires approaches that can enable ICH practitioners to reimagine and recreate ICH for modern contexts. To address this, we created workshops where experienced practitioners of traditional Chinese cuisine co-created recipes using GenAI tools and realized the dishes. We found that GenAI inspired ICH practitioners to innovate recipes based on traditional workflows for broader audiences and adapt to modern dining contexts. However, GenAI-inspired co-creation posed challenges in maintaining the accuracy of original ICH workflows and preserving traditional flavors in the culinary outcomes. This study offers implications for designing human-AI collaborative processes for safeguarding and enhancing culinary ICH.
Working with Patients Living with Obesity in the Intensive Care Unit: A Study of Nurses’ Experiences
Creators: name: Shea, Jacqueline M.
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2014
Subjects: subject: ICU
subject: Intensive Care
subject: Nurses
subject: Nursing
subject: FOS: Health sciences
subject: Obesity
subject: Othering

description: Nurses who work in the intensive care settings (or units, ICU) in Canada encounter a growing number of patients living with obesity (PLWO) in clinical practice. Many authors suggest that the number of PLWO who are admitted to the ICU has increased significantly because obesity is on the rise in Canada. PLWO are thought to be at a higher risk for developing chronic illnesses and life-threatening complications that require an admission to the ICU. They are also more likely to develop postoperative complications that require life-sustaining treatments, invasive hemodynamic monitoring and evaluation, assistive devices, pharmacological interventions, parenteral nutrition, fluid and electrolyte management, and prolonged admission with associated risks of complications. Yet, there is limited research on the experience of nurses providing care to PLWO. The goal of this qualitative study was to examine the experiences of ICU nurses who work with PLWO and how these experiences affect the way they provide care. More specifically, this study was designed to describe and explore the inclusionary and exclusionary practices developed by nurses providing care to PLWO by drawing Canales’ (2000) Othering framework. Lastly, an additional goal of this study was to document the needs of ICU nurses with respect to the care of PLWO and areas of improvement in the ICU. A total of 11 ICU nurses were interviewed for this study. Data analysis followed the principles of Applied Thematic Analysis (ATA) and revealed four themes. The first theme describes how the PLWO become “Other” in the ICU context. The second theme focuses on exclusionary Othering and how it manifests itself in the way PLWO are differentiated, cared for, and viewed in the ICU context. The third theme sheds light on inclusionary Othering in the form of strategies that are used by ICU nurses to engage with PLWO in a way that is inclusive and transformative. Finally, the last theme concentrates on the ICU environment itself and the resources available (or not available) to nurses, with a particular emphasis on the needs of nurses who provide care to PLWO.
Phosphorylation of Filamin A by Cdk1/cyclin B1 Regulates Filamin A Subcellular Localization and is Important for Daughter Cell Separation
Creators: name: Szeto, Sandy
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2014
Subjects: Actin cytoskeleton remodeling
Mitotic cell rounding
Filamin A
Cyclin-dependent kinase 1
Mitosis
Phosphorylation

description: In cell culture, entry into mitosis of many adherent mammalian cells is accompanied by substantial changes in cellular architecture. Flat, spread-out interphase cells detach from the extracellular matrix and become more spherical. These changes in cell shape are mediated by rearrangements in the actin cytoskeleton, a dynamic network of actin filaments that are organized by actin-binding proteins. Filamin A (FLNa) is a 280 kD actin-binding protein that crosslinks actin filaments into parallel bundles or three-dimensional orthogonal networks. We previously identified FLNa as an in vitro substrate of cyclin-dependent kinase 1 (Cdk1), a kinase that regulates entry into mitosis, and hypothesized that Cdk1 phosphorylation of FLNa regulates mitotic actin remodelling. Using mass spectrometry and a p-FLNa antibody, we show that FLNa is phosphorylated in vivo in HeLa cells on multiple Cdk1 sites, including serines 1084, 1459 and 1533. All three sites match the phosphorylation consensus sequence of Cdk1. We further show that p-FLNa is almost fully dephosphorylated by anaphase, consistent with it being a cell cycle-regulated substrate. Using a phospho-specific antibody, we find that p-FLNa has decreased cortical actin localization compared to total FLNa in mitotic cells. To investigate the functional role of mitotic FLNa phosphorylation, we mutated serines 1084, 1459 and 1533 to nonphosphorylatable alanine and expressed this FLNa mutant (FLNa-S1084A, S1459A, S1533A, referred to as “FLNa-AAA GFP”) in FLNa-deficient human M2 melanoma cells. FLNa-AAA GFP-expressing cells have enhanced FLNa-AAA GFP localization at sites of contact between daughter cells and this correlates with defects in cell division and impaired cell migration. Therefore, mitotic delocalization of cortical FLNa is critical for successful cell division and interphase cell behaviour.
Multifunctional Adaptive NS1 Mutations Are Selected Upon Influenza A Virus Evolution in the Mouse
Creators: name: Forbes, Nicole E
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2012
Subjects: Influenza
Evolution
Virulence
NS1
Interferon
Adaptation
virus gene expression
viral genetics

description: Influenza A virus (IAV) can evolve from low virulence in animal hosts to become highly virulent in humans. Pandemic Influenza A viruses such as the 1918 Spanish Influenza caused over 50 million deaths worldwide. However the genetic determinants of IAV host adaptation and virulence are largely uncharacterized. The IAV NS1 protein is a multifunctional interferon antagonist and a known virulence factor. We hypothesized that NS1 mutations selected upon IAV evolution to a novel host contribute to host adaptation by mechanisms involving increased gene expression and IFN antagonism. To this end, I phenotypically characterized the NS1 mutations selected upon adaptation of A/Hong Kong/1/1968 (H3N2) (HK-wt) to increased virulence in the mouse. Sequencing the NS genome segment of mouse-adapted variants revealed eleven mutations in the NS1 gene and four in the overlapping NEP gene. Using the HK-wt virus and reverse genetics to express recombinant HK NS1 mutant viruses, I demonstrated that all NS1 mutations were adaptive and enhanced virus replication (up to 100 fold) in mouse cells and/or lungs. All but one NS1 mutant was associated with increased virulence measured by survival and weight loss in the mouse. Ten of twelve NS1 mutants significantly enhanced IFN-β antagonism to reduce the level of IFN-β production relative to HK-wt in infected mouse lungs at 1 day post infection, where nine mutants induced viral yields in the lung that were ≥ HK-wt (up to 16 fold increase). Eight of 12 NS1 mutants had decreased binding affinity to the cleavage and polyadenylation specificity factor (CPSF30). The majority of mutant NS1 genes demonstrated increased viral polymerase activity and viral protein production in mouse cells. Viral protein production and viral growth were also assessed in human and canine cell lines; however these adaptive phenotypes were more robust in infected mouse cells. Adaptive NS1 mutations also increased cytoplasmic cellular localization of the NS1 protein in infected cells in a host cell-specific manner. Evaluation of phenotypic trends associated with the NS1 mutants demonstrated an inverse correlation between CPSF30 binding affinity and viral polymerase activity enhancement. This study demonstrates that NS1 is a multifunctional virulence factor subject to adaptive evolution.
Polymorphisms in the airway epithelium related genes CDHR3 and EMSY are associated with asthma susceptibility
Creators: name: Zhang, Miaomiao
name: Chen, Guo
name: Wang, Yu
name: Wu, Shou-Quan
name: Sandford, Andrew J.
name: He, Jian-Qing
Publisher: name: BioMed Central
Year: 2020

description: Background: As a main line of defense of the respiratory tract, the airway epithelium plays an important role in the pathogenesis of asthma. CDHR3 and EMSY were reported to be expressed in the human airway epithelium. Although previous genome-wide association studies found that the two genes were associated with asthma susceptibility, similar observations have not been made in the Chinese Han population. Methods: A total of 300 asthma patients and 418 healthy controls unrelated Chinese Han individuals were enrolled. Tag-single nucleotide polymorphisms (Tag-SNPs) were genotyped and the associations between SNPs and asthma risk were analyzed by binary logistic regression analysis. Results: After adjusting for confounding factors, the A allele of rs3847076 in CDHR3 was associated with increased susceptibility to asthma (OR = 1.407, 95% CI: 1.030–1.923). For the EMSY gene, the T alleles of both rs2508746 and rs12278256 were related with decreased susceptibility to asthma (additive model: OR = 0.718, 95% CI: 0.536–0.961; OR = 0.558, 95% CI: 0.332–0.937, respectively). In addition, the GG genotype of rs1892953 showed an association with increased asthma risk under the recessive model (OR = 1.667, 95% CI: 1.104–2.518) and the GATCTGAGT haplotype in EMSY was associated with reduced asthma risk (P = 0.037). Conclusions: This study identified novel associations of rs3847076 in CDHR3, as well as rs1892953, rs2508746 and rs12278256 in EMSY with adult asthma susceptibility in the Chinese Han population. Our observations suggest that CDHR3 and EMSY may play important roles in the pathogenesis of asthma in Chinese individuals. Further study with larger sample size is needed.
Undergraduate Students’ Connections Between the Embodied, Symbolic, and Formal Mathematical Worlds of Limits and Derivatives: A Qualitative Study Using Tall’s Three Worlds of Mathematics
Creators: name: Smart, Angela
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2013
Subjects: mathematics education
multiple representations
university calculus
cognitive development
Three Worlds of Mathematics
connecting representations
embodiment

description: Calculus at the university level is taken by thousands of undergraduate students each year. However, a significant number of students struggle with the subject, resulting in poor problem solving, low achievement, and high failure rates in the calculus courses overall (e.g., Kaput, 1994; Szydlik, 2000; Tall, 1985; Tall & Ramos, 2004; White & Mitchelmore, 1996). This is cause for concern as the lack of success in university calculus creates further barriers for students who require the course for their programs of study. This study examines this issue from the perspective of Tall’s Three Worlds of Mathematics (Tall, 2004a, 2004b, 2008), a theory of mathematics and mathematical cognitive development. A fundamental argument of Tall’s theory suggests that connecting between the different mathematical worlds, named the Embodied-Conceptual, Symbolic-Proceptual, and Formal-Axiomatic worlds, is essential for full cognitive development and understanding of mathematical concepts. Working from this perspective, this research examined, through the use of calculus task questions and semi-structured interviews, how fifteen undergraduate calculus students made connections between the different mathematical worlds for the calculus topics of limits and derivatives. The analysis of the findings suggests that how the students make connections can be described by eight different Response Categories. The study also found that how the participants made connections between mathematical worlds might be influenced by the type of questions that are asked and their experience in calculus courses. I infer that these Response Categories have significance for this study and offer potential for further study and educational practice. I conclude by identifying areas of further research in regards to calculus achievement, the Response Categories, and other findings such as a more detailed study of the influence of experience.
title: "I Can See Now" Understanding Literacy Practices in the Context of Afghanistan
Creators: name: Anastacio, Anita
Publisher: name: University of Massachusetts Amherst
Year: 2006
Subjects: subject: Education
subject: International and Comparative Education

description: I have structured my paper as follows: Chapter 2 of this paper provides an overview of the historical, political and educational context of Afghanistan out of which a project like Learning of Life, a health focused literacy project and the focus of my study emerged. Chapter 3 familiarizes the reader with the theoretical discussions that have shaped the concept of literacy. I will do that by using a framework used by UNESCO (2006) that presents the theory using four approaches to understand the meaning of literacy. Chapter 4 presents the methodology of the research I carried out by looking at the type of approach I used, its rationale and the tools and methods of analysis I used. Chapter 5 examines how policy makers and practitioners who have supported, designed and implemented Learning for Life, are influenced by hegemonic discourses on development, gender and literacy of its institutions. Here I use one of the four approaches: "literacy understood as text." Chapter 6 explores current literacy practices in a village where women were enrolled in the LFL literacy class. Using the approach of the New Literacy Studies (Street, 1984) it moves from practices to concentrate literacy events. Chapter 7 reviews the original intent of my research and looks at how thoughtfully examining and understanding literacy and numeracy practices is a first critical step in planning meaningful literacy programs for Afghan women. This chapter also discusses implications of policy and programming offering suggestions for further research and inquiry.
The Cult of Fashion Brands in China and the Application of Microblogging
Creators: name: Han, Lu
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2013
Subjects: microblogging
fashion brands
China
Uses and gratifications
Content analysis

description: In China, an increasing number of individuals and companies are adopting microblogging, a popular form of social media, in order to connect and interact with other people, and recent online events indicate the power of microblogging in Chinese society. Holding the belief that microblogging brings out the interactive nature of new media as well as the audiences, many companies are exploring microblogging in order to better communicate with their audiences. However, very little is known about how those brands use microblogging to promote themselves and what the audiences’ preferences are on this platform. Employing uses and gratifications and feminism theories, this study examined how fashion brands use Weibo.com, one of the main microblogging platforms in China, to promote themselves and what the Chinese women, the main audience of both Weibo.com and fashion brands, ask for from fashion brands’ tweets. The quantitative content analysis of the tweets of three major fashion brands, namely Burberry, Louis Vuitton, and Bvlgari, shows the general pattern of how microblogging are being deployed. A further investigation was conducted through ethnographic content analysis in order to examine the implicit values conveyed by fashion brand’s tweets and the audiences’ preferences towards these values. Results from the analyses revealed that the prevailing topics covered in the fashion brands’ tweets included their products, related celebrities, and the brands’ events or projects, and fashion brands usually combined several topics in one tweet in order to provide more information to the audiences. Taken a deeper look at the latent message of the tweets, fashion appears to play a positive role in emancipating contemporary Chinese women.
Anomalous valley Hall effect in monolayer chromium-based triple-Q magnets
Creators: name: Jiang, Xiu-Cai
name: Qiao, Li-Ya
name: Zhang, Yu-Zhong
Publisher: name: arXiv
Year: 2025
Subjects: subject: Materials Science (cond-mat.mtrl-sci)
subject: Strongly Correlated Electrons (cond-mat.str-el)
subject: Computational Physics (physics.comp-ph)
subject: FOS: Physical sciences

description: Using the density functional theory calculations, we predict that several monolayer chromium-based materials exhibit a triple-Q tetrahedral magnetic insulating ground state. By studying the effect of biaxial strain on monolayer CrSi$\rm{_2}$P$\rm{_4}$ under various on-site Coulomb interactions, we reveal that this magnetic insulating state, sandwiched between the itinerant $120^{\circ}$ coplanar noncollinear antiferromagnetic and ferromagnetic states, originates from the competition between antiferromagnetic exchange and double exchange interactions of Cr 3$d$ electrons which can also be applied to account for the ground states in other chromium-based materials. Remarkably, anomalous valley Hall effect with giant valley splitting is discovered in the magnetic states of these inversion-asymmetric systems without requiring spin-orbit coupling or net magnetization. Our findings open a new avenue towards exploring monolayer materials for valleytronics.
description: 8 pages, 4 figures
Anisotropic Bianchi-I cosmological model with SU(2)-symmetric Yang-Mills field of the magneto-electric type
Creators: name: Balakin, Alexander B.
name: Kiselev, Gleb B.
Publisher: name: arXiv
Year: 2025
Subjects: subject: General Relativity and Quantum Cosmology (gr-qc)
subject: FOS: Physical sciences

description: An anisotropic cosmological model with a gauge field, presented by spatially homogeneous SU(2)-symmetric Yang-Mills potentials, is considered. Bianchi-I model with axial symmetry is analyzed; a new exact solution, which describes the early Universe on the stage of domination of the gauge field of the magneto-electric type, is obtained.
description: 4 pages, 0 figures, short communication based on the talk, presented on the Conference RUSGRAV-18
Adding team-based financial incentives to the Carrot Rewards physical activity app increases daily step count on a population scale: a 24-week matched case control study
Creators: name: Pearson, Emma
name: Prapavessis, Harry
name: Higgins, Christopher
name: Petrella, Robert J.
name: White, Lauren
name: Mitchell, Marc
Publisher: name: BioMed Central
Year: 2020

description: Background: Mobile health applications (mHealth apps) targeting physical inactivity have increased in popularity yet are usually limited by low engagement. This study examined the impact of adding team-based incentives (Step Together Challenges, STCs) to an existing mHealth app (Carrot Rewards) that rewarded individual physical activity achievements. Methods: A 24-week quasi-experimental study (retrospective matched pairs design) was conducted in three Canadian provinces (pre-intervention: weeks 1–12; intervention: weeks 13–24). Participants who used Carrot Rewards and STCs (experimental group) were matched with those who used Carrot Rewards only (controls) on age, gender, province and baseline mean daily step count (±500 steps/d). Carrot Rewards users earned individual-level incentives (worth $0.04 CAD) each day they reached a personalized daily step goal. With a single partner, STC users could earn team incentives ($0.40 CAD) for collaboratively reaching individual daily step goals 10 times in seven days (e.g., Partner A completes four goals and Partner B completes six goals in a week). Results: The main analysis included 61,170 users (mean age = 32 yrs.; % female = 64). Controlling for pre-intervention mean daily step count, a significant difference in intervention mean daily step count favoured the experimental group (p < 0.0001; ηp² = 0.024). The estimated marginal mean group difference was 537 steps per day, or 3759 steps per week (about 40 walking min/wk). Linear regression suggested a dose-response relationship between the number of STCs completed (app engagement) and intervention mean daily step count (adjusted R² = 0.699) with each new STC corresponding to approximately 200 more steps per day. Conclusion: Despite an explosion of physical activity app interest, low engagement leading to small or no effects remains an industry hallmark. In this paper, we found that adding modest team-based incentives to the Carrot Rewards app increased mean daily step count, and importantly, app engagement moderated this effect. Others should consider novel small-teams based approaches to boost engagement and effects.
Trends in the Exchange of CO2 and CH4 between the Atmosphere and Eastern Canadian Subarctic and Arctic Ecosystems
Creators: name: Pilote, Martin
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2015
Subjects: Gas exchange
Carbon balance
NEE
CO2
CH4
Environmental factor
Temperature
Precipitation
Arctic
Subarctic
Spatial trend
Temporal trend

description: Significant warming of Arctic and northern regions is ongoing and may greatly alter the carbon cycle of these regions. During the International Polar Year, an extensive study was carried out in the Eastern Canadian subarctic and Arctic in order to characterize CO2 and CH4 exchanges from these potentially sensitive ecosystems. The main objectives of this study were to identify the land cover and environmental factors leading to greatest CO2 and CH4 emissions in a highly heterogeneous subarctic landscape, to quantify interannual variability in the net ecosystem exchange of CO2 (NEE) in subarctic forest tundra and investigate the weather conditions that increase net uptake of CO2, and finally, to evaluate the general trends of mid-summer NEE along a latitudinal gradient spanning from 55° to the 72° north. At the landscape level, CO2 and CH4 exchanges showed large variability. Although CH4 emissions were greatest in wetlands, their areal coverage is small in the Kuujuarapik area and limited the influence of these CH4 sources. At the ecosystem level, large-scale atmospheric processes controlled growing season length and cumulative growing degree days which greatly influenced annual and seasonal NEE trends. The subarctic forest tundra near Kuujuarapik was a net source of CO2 in all 3 study years but the source strength was least with the greatest growing degree days while the length of the snow-free period appeared to be less important. Across a latitudinal gradient covering subarctic forest tundra to Arctic tundra, variations in summer NEE could be linked to surface organic carbon content with higher net CO2 uptake at sites with greater soil organic carbon. Warmer days tended to correlate with smaller daily net CO2 uptake (or greater net CO2 losses) but overall, warmer growing seasons reduced the net losses of CO2 on an annual basis. Carbon fluxes in Eastern Canadian subarctic and Arctic regions are highly variable in space and time but these observations help establish a baseline for future examinations of how these carbon exchanges may change with further warming.
Supplementary document for Batch fabrication and compact integration of customized multispectral filter arrays towards snapshot imaging - 5425475.pdf
Creators: name: Yu, Xiaochang
name: Su, Yang
name: Song, Xuankun
name: Wang, Fei
name: Gao, Bo
name: Yu, Yiting
Publisher: name: Optica Publishing Group
Year: 2025
Subjects: Uncategorized

description: Details on the fabrication of multispectral filter arrays.
MEF2 Isotypes During Skeletal Myogenesis
Creators: name: Reilly, Katherine
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2015
Subjects: MEF2
Skeletal Myogenesis
PRMT1

description: The MEF2 family of transcription factors (MEF2A, MEF2C, and MEF2D) are crucial during skeletal muscle differentiation. Although the roles of MEF2D isoforms are well established, the roles of MEF2A and MEF2C are not as well understood. This thesis, we investigated the expression, localization, and function of MEF2A and MEF2C, using specific antibodies. While MEF2A is expressed in both proliferating and differentiated myoblasts, protein levels of MEF2C were only detected during differentiation. During early stages of differentiation MEF2A is expressed in both the cytoplasm and the nucleus. However during later stages of differentiation, it is localized predominately in the nucleus. MEF2C appears to be localized differently depending on which isoform is being investigated. Using an affinity purification and mass spectrometry based approach we identified PRMT1 as a unique interacting protein with MEF2A during skeletal muscle differentiation. PRMT1 is a protein arginine methyltransferase which mediates the addition of methyl groups onto various proteins including histone H4 arginine 3 (H3R4) which is associated with gene activation. Both MEF2A and PRMT1 occupy genomic targets of MEF2A. Inhibition of PRMT1 with a specific inhibitor delays C2C12 myoblast differentiation in the early stages of differentiation but no effect was observed during late stage differentiation. The MEF2 family of transcription factors show distinct but overlapping function during skeletal muscle differentiation.
Investigation of Cis and Trans-acting Transcriptional Regulatory Factors and Signaling Pathways of Parkin
Creators: name: Ao, Hei Sio
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2015
Subjects: Parkinson's disease
Parkin
Nrf2

description: Parkin gene is associated with the development of autosomal recessive juvenile parkinsonism (Kitada et al., 1998) which is a common form of familial Parkinson’s disease (Klein and Schlossmacher, 2006). Since Parkin has multiple cell protective effects, increasing the expression level of Parkin in the brain might be able to rescue cells in danger, which in turn might prevent or slow down the development of Parkinson’s disease (Ulusoy and Kirik, 2008). In order to increase Parkin expression, it is important to understand the transcriptional mechanisms regulating Parkin expression (Maston et al., 2006). Since human Parkin is very big (~1.4 Mb) (Asakawa et al., 2001), in this study we use the smaller Fugu parkin gene, which is an ortholog of human Parkin (Yu et al., 2005), to search for the transcriptional factors and signaling pathways regulating Parkin expression. We have cloned vertebrate constructs that allow for the monitoring of an entire genomic Fugu parkin gene tagged with a reporter (eGFP or luciferase) in mammalian cells; and have established cellular model for studying the expression. According to the “TRANSFAC” transcription factor database, as well as “TFBIND” and “TFSEARCH” softwares (Wingender et al., 1996; Heinemeyer et al., 1998; Heinemeyer et al., 1999; Tsunoda and Takagi, 1999; Akiyama 1995), potential Nrf2 binding sites are conserved in the promoters of mammalian parkin (including human Parkin and mouse parkin) and in Fugu parkin. In this study, we could not find a link between the presence of the potential Nrf2 binding site(s) in the parkin promoter and the up-regulation of parkin; and we could not find an association between the Nrf2 pathway activation and the induction of parkin under the specific experimental conditions.
The Mechanisms of Protective Function of DJ-1 in Parkinson’s Models of Neuronal Loss: VHL and PON2
Creators: name: Parsanejad, Mohammad
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2013
Subjects: Parkinson's disease
Neurodegeneration
MPP+
DJ-1
PON2
VHL
HIF-1

description: Parkinson's disease (PD) is the most common neurodegenerative motor disorder, whose clinical features are rest tremor, bradykinesia, muscular rigidity and postural instability. Although most reported cases are sporadic, a handful of familial cases and their causative genes have been identified. Loss-of-function mutations in DJ-1, one of these genes, are responsible for 1% of familial PD cases. Our laboratory has previously reported that DJ-1- lacking neurons are sensitive to oxidative stress, induced by hydrogen peroxide or the neurotoxin MPTP. To investigate the possible mechanisms through which DJ-1 protects against oxidative stress, we performed a proteomic screen and identified Von Hippel Lindau (VHL) and Paraoxonase2 (PON2) as potential DJ-1 interacting partners. VHL is an E3 ubiquitin ligase which, in normal conditions, poly-ubiquitinates HIF-1 , a subunit of a master hypoxic/oxidative stress transcription factor, whose function is protective in oxidative and hypoxic stresses. In the present study, we provided further evidence of interaction of DJ-1 with VHL. We also demonstrated that HIF-1 protein level, as an indicator of VHL activity, is lower in cells lacking DJ-1, suggesting the inhibitory role of DJ-1 on VHL. Our in vitro studies also showed that DJ-1 inhibits ubiquitin ligase activity of VHL on HIF-1 by reducing the VHL-HIF-1 interaction. Importantly, accumulation of HIF-1 protects embryonic cortical neurons against MPP+ induced neuronal death. Finally, we confirmed the impairment of HIF-1 response to oxidative stress in human lymphoblastoids of DJ-1-linked PD cases. In the second part of this study, we demonstrated the interaction of DJ-1 and PON2. Interestingly, PON2 lactonase activity is reduced in DJ-1 deficient cells which could be rescued by re-introduction of DJ-1, suggesting a modulating role of DJ-1 on PON2 activity. In addition, PON2 deficiency, like DJ-1 deficiency, hypersensitizes neurons to MPP+, which could be rescued by over-expression of PON2 in both cases. Taken together, our data provide evidence that DJ-1 exerts its protective role by inhibiting VHL activity, enhancing HIF-1 stability, and increasing PON2 pro-survival function in PD models.
Numerical Modeling of Thermal/Saline Discharges in Coastal Waters
Creators: name: Kheirkhah Gildeh, Hossein
Publisher: name: Université d'Ottawa / University of Ottawa
Year: 2013
Subjects: Numerical Modeling
OpenFOAM
Effluent Discharge
Desalination Plant
Dense Jets
Buoyant Wall Jets
Turbulence Models
Mixing and Dispersion
RSM

description: Liquid waste discharged from industrial outfalls is categorized into two major classes based on their density. One type is the effluent that has a higher density than that of the ambient water body. In this case, the discharged effluent has a tendency to sink as a negatively buoyant jet. The second type is the effluent that has a lower density than that of the ambient water body and is hence defined as a (positively) buoyant jet that causes the effluent to rise. Negatively/Positively buoyant jets are found in various civil and environmental engineering projects: discharges of desalination plants, discharges of cooling water from nuclear power plants turbines, mixing chambers, etc. This thesis investigated the mixing and dispersion characteristics of such jets numerically. In this thesis, mixing behavior of these jets is studied using a finite volume model (OpenFOAM). Various turbulence models have been applied in the numerical model to assess the accuracy of turbulence models in predicting the effluent discharges in submerged outfalls. Four Linear Eddy Viscosity Models (LEVMs) are used in the positively buoyant wall jet model for discharging of heated waste including: standard k-ε, RNG k-ε, realizable k-ε and SST k-ω turbulence models. It was found that RNG k-ε, and realizable k-ε turbulence models performed better among the four models chosen. Then, in the next step, numerical simulations of 30˚ and 45˚ inclined dense turbulent jets in stationary ambient water have been conducted. These two angles are examined in this study due to lower terminal rise height for 30˚ and 45˚, which is very important for discharges of effluent in shallow waters compared to higher angles. Five Reynolds-Averaged Navier-Stokes (RANS) turbulence models are applied to evaluate the accuracy of CFD predictions. These models include two LEVMs: RNG k-ε, and realizable k-ε; one Nonlinear Eddy Viscosity Model (NLEVM): Nonlinear k-ε; and two Reynolds Stress Models (RSMs): LRR and Launder-Gibson. It has been observed that the LRR turbulence model as well as the realizable k-ε model predict the flow more accurately among the various turbulence models studied herein.
Understanding the roles of housing costs and housing assistance in creating employment disincentives
Creators: name: Hulse, Kathleen
name: Randolph, Bill
name: Toohey, Matthew
name: Beer, Gillian
name: Lee, Rob
Publisher: name: Swinburne
Year: 2024

description: There is a growing concern and interest in government, and more broadly, in the way that housing assistance and income support systems interact to provide incentives or disincentives for unemployed people to enter paid employment (Industry Commission 1993; Croce 2000). Research outside Australia has highlighted the critical role that housing assistance plays in the decisions that unemployed people make when offered work (Hirsch 1994; Randolph 1994; Giles et al. 1996; Ford, Kempson and England 1996; Wilcox and Sutherland 1997). This research suggests that decisions about moving into paid employment typically involve trade offs between the financial, personal and family benefits expected from participation in paid employment relative to costs in terms of loss of income support payments and impact on personal and family well being. In particular, these studies have pointed to the complex interactions between incomes from paid employment and government benefits, including the combined effect of withdrawal of government benefits and housing assistance, the type and nature of housing costs, and the behaviour of those seeking employment. [Background]
title: Elektro- und Elektronikabfallverwertung in Kaliningrad Phase II
title: Abschlussbericht (Final Report, German)
titleType: AlternativeTitle
Creators: name: Knoch, Joachim
name: Ebbing, Jochen
Publisher: name: My University
Year: 2014
Subjects: subject: Beratungshilfeprogramm
subject: Kaliningrad
subject: Elektro- und Elektronikabfall
subject: Abfall | Ressourcen
subject: Nachhaltigkeit | Strategien | Internationales
subject: Waste | Resources
subject: Sustainability | Strategies | International matters

description: Ausgehend von dem erarbeiteten und zur weiteren Betrachtung ausgewählten ⁠Szenario⁠ „Erweiterung der bestehenden Entsorgungsmöglichkeiten“ wurde die Stadtverwaltung Kaliningrad in diesem Projekt zu Erarbeitung und Umsetzung der notwendigen Maßnahmen zur Erfassung der Elektro- und Elektronikabfälle beraten. Dabei sollten technische und organisatorische Planungsunterlagen für den Aufbau eines zentralen Entsorgungssystems für Elektro- und Elektronikabfällen beschrieben werden.
О проектировании программ и алгоритмов управления производственной поточной линией
Creators: name: Пигнастый, Олег Михайлович
name: Ходусов, Валерий Дмитриевич
Publisher: name: Zenodo
Year: 2020
Subjects: PDE-модель
производственная линия
PDE-модель производства
системы управления производством
незавершенное производство
массовое производство
PDE-model production line

description: В докладе представлены основные этапы проектирования программ и алгоритмов управления потоковыми параметрами производственной линии. Показаны этапы построения распределенной модели поточной производственной линии. Обсуждается связь предметно-технологического и потокового уровня описания. Изложены методы построения программ управления для распределенной модели поточной линии. На рисунке показаны разработанные и обсуждаемые в докладе методы оптимального управления потоковыми параметрами производственной линии.
description: {"references": ["1. \u041f\u0438\u0433\u043d\u0430\u0441\u0442\u044b\u0439 \u041e. \u041c. \u041e \u043d\u043e\u0432\u043e\u043c \u043a\u043b\u0430\u0441\u0441\u0435 \u0434\u0438\u043d\u0430\u043c\u0438\u0447\u0435\u0441\u043a\u0438\u0445 \u043c\u043e\u0434\u0435\u043b\u0435\u0439 \u043f\u043e\u0442\u043e\u0447\u043d\u044b\u0445 \u043b\u0438\u043d\u0438\u0439 \u043f\u0440\u043e\u0438\u0437\u0432\u043e\u0434\u0441\u0442\u0432\u0435\u043d\u043d\u044b\u0445 \u0441\u0438\u0441\u0442\u0435\u043c / \u041e. \u041c. \u041f\u0438\u0433\u043d\u0430\u0441\u0442\u044b\u0439 // \u041d\u0430\u0443\u0447\u043d\u044b\u0435 \u0432\u0435\u0434\u043e\u043c\u043e\u0441\u0442\u0438 \u0411\u0435\u043b\u0433\u043e\u0440\u043e\u0434\u0441\u043a\u043e\u0433\u043e \u0433\u043e\u0441\u0443\u0434\u0430\u0440\u0441\u0442\u0432\u0435\u043d\u043d\u043e\u0433\u043e \u0443\u043d\u0438\u0432\u0435\u0440\u0441\u0438\u0442\u0435\u0442\u0430. \u0411\u0435\u043b\u0433\u043e\u0440\u043e\u0434: \u0411\u0413\u0423. - 2014. - \u2116 31/1. - \u0421. 147-157", "2. \u041f\u0438\u0433\u043d\u0430\u0441\u0442\u044b\u0439 \u041e. \u041c. \u041e\u0431\u0437\u043e\u0440 \u043c\u043e\u0434\u0435\u043b\u0435\u0439 \u0443\u043f\u0440\u0430\u0432\u043b\u044f\u0435\u043c\u044b\u0445 \u043f\u0440\u043e\u0438\u0437\u0432\u043e\u0434\u0441\u0442\u0432\u0435\u043d\u043d\u044b\u0445 \u043f\u0440\u043e\u0446\u0435\u0441\u0441\u043e\u0432 \u043f\u043e\u0442\u043e\u0447\u043d\u044b\u0445 \u043b\u0438\u043d\u0438\u0439 \u043f\u0440\u043e\u0438\u0437\u0432\u043e\u0434\u0441\u0442\u0432\u0435\u043d\u043d\u044b\u0445 \u0441\u0438\u0441\u0442\u0435\u043c / \u041e.\u041c. \u041f\u0438\u0433\u043d\u0430\u0441\u0442\u044b\u0439 // \u041d\u0430\u0443\u0447\u043d\u044b\u0435 \u0432\u0435\u0434\u043e\u043c\u043e\u0441\u0442\u0438 \u0411\u0435\u043b\u0433\u043e\u0440\u043e\u0434\u0441\u043a\u043e\u0433\u043e \u0433\u043e\u0441\u0443\u0434\u0430\u0440\u0441\u0442\u0432\u0435\u043d\u043d\u043e\u0433\u043e \u0443\u043d\u0438\u0432\u0435\u0440\u0441\u0438\u0442\u0435\u0442\u0430. \u0411\u0435\u043b\u0433\u043e\u0440\u043e\u0434: \u0411\u0413\u0423. - 2015. - \u2116 34/1. \u0421.137-152"]}
An in vitro bioengineered model of the human arterial neurovascular unit to study neurodegenerative diseases
Creators: name: Robert, Jerome
name: Weilinger, Nicholas L.
name: Cao, Li-Ping
name: Cataldi, Stefano
name: Button, Emily B.
name: Stukas, Sophie
name: Martin, Emma M.
name: Seibler, Philip
name: Gilmour, Megan
name: Caffrey, Tara M.
name: Rowe, Elyn M.
name: Fan, Jianjia
name: MacVicar, Brian
name: Farrer, Matthew J.
name: Wellington, Cheryl L.
Publisher: name: BioMed Central
Year: 2020

description: Introduction: The neurovascular unit (NVU) – the interaction between the neurons and the cerebrovasculature – is increasingly important to interrogate through human-based experimental models. Although advanced models of cerebral capillaries have been developed in the last decade, there is currently no in vitro 3-dimensional (3D) perfusible model of the human cortical arterial NVU. Method: We used a tissue-engineering technique to develop a scaffold-directed, perfusible, 3D human NVU that is cultured in native-like flow conditions that mimics the anatomy and physiology of cortical penetrating arteries. Results: This system, composed of primary human vascular cells (endothelial cells, smooth muscle cells and astrocytes) and induced pluripotent stem cell (iPSC) derived neurons, demonstrates a physiological multilayer organization of the involved cell types. It reproduces key characteristics of cortical neurons and astrocytes and enables formation of a selective and functional endothelial barrier. We provide proof-of-principle data showing that this in vitro human arterial NVU may be suitable to study neurovascular components of neurodegenerative diseases such as Alzheimer’s disease (AD), as endogenously produced phosphorylated tau and beta-amyloid accumulate in the model over time. Finally, neuronal and glial fluid biomarkers relevant to neurodegenerative diseases are measurable in our arterial NVU model. Conclusion: This model is a suitable research tool to investigate arterial NVU functions in healthy and disease states. Further, the design of the platform allows culture under native-like flow conditions for extended periods of time and yields sufficient tissue and media for downstream immunohistochemistry and biochemistry analyses.