Bradyrhizobium altum Avontuur &amp; Palmer &amp; Beukes &amp; Chan &amp; Tasiya &amp; Zyl &amp; Coetzee &amp; Stepkowski &amp; Venter &amp; Steenkamp 2022, sp. nov.
Creators: name: Avontuur, Juanita R.
name: Palmer, Marike
name: Beukes, Chrizelle W.
name: Chan, Wai Y.
name: Tasiya, Taponeswa
name: Zyl, Elritha van
name: Coetzee, Martin P. A.
name: Stepkowski, Tomasz
name: Venter, Stephanus N.
name: Steenkamp, Emma T.
Publisher: name: Zenodo
Year: 2021
Subjects: Biodiversity
Taxonomy
Bacteria
Proteobacteria
Alphaproteobacteria
Rhizobiales
Bradyrhizobiaceae
Bradyrhizobium
Bradyrhizobium altum

description: <i>4.3. Description of Bradyrhizobium altum sp. nov.</i> <i>Bradyrhizobium altum</i> (al’ tum. L. neut. adj. <i>altum</i>, high, referring to the high-altitude region where this species was isolated from). Cells are motile, Gram-negative rods and is approximately 1.29 × 0.45 μm in size. Colonies are circular, convex, opaque, cream in colour and <i>&lt;</i> 1 mm in diameteron YMA after 7 daysincubation at 28 ◦ C. The pH range for growth on YMA is 5 to 10 and growth can occur between 15 and 37 ◦ Cand thesestrainscangrowinthe presenceof upto 2.0 % (w/v) NaCl. Reduction of nitrates, β- glucosidase urease, tested positive. It is also positive for the production of L-arabinose, Tween 40, Tween 80, adonitol, N-acetyl-D-glucosamine, D-cellobiose, D-arabitol, D-galactose, D- and L-fructose, m-inositol, D-mannitol, maltose, D-mannose, Draffinose, D-melibiose, L-rhamnose, D-sorbitol, sucrose, xylitol, Dtrehalose, turanose, methyl pyruvate, D-galacturonic acid, acetic acid, malonic acid, propionic acid, D-saccharic acid, quinic acid, sebacic acid, L-asparagine, D- and L-alanine, L-leucine, L-proline, L-ornithine and glycerol. Negative reactions were recorded for glucose fermentation, indole production, β- galactosidase activity and L-arginine dihydrolase. It is also negative for the assimilation of N-acetyl-glucosamine, potassium gluconate, D-maltose, adipic acid, capric acid, malic acid, phenylacetic acid, glycogen, α- cyclodextrin, L-arabinose, i-erythritol, Lthreonine, D- and L-serine, glucose-1-phosphate and glucose-6- phosphate. Strain is resistant to penicillin (10 μg/ml), ampicillin (10 μg/ml), gentamycin (10 μg/ml), chloramphenicol (30 μg/ml) and neomycin (10 μg/ml) and is susceptible to kanamycin (30 μg/ml), erythromycin (30 μg/ml), streptomycin (10 and 25 μg/ml) and tetracycline (30 μg/ml). The type strain Pear77 T (=SARCC 754 = LMG 31407), was isolated from root nodules of <i>Pearsonia obovata</i> collected in the Dullstroom area of the Mpumalanga Province, South Africa. This species forms effective nodules on cowpea (<i>Vigna unguiculata</i>) and siratro (<i>Macroptilium atropurpureum</i>). The DNA G + Ccontent of the type strain is 63.3 mol%. The GenBank/EMBL/DDBJ accession numbers for the genome sequence and gene sequences for this type strain are: whole genome (JACMYP000000000) and 16S rRNA (LR877298), <i>atpD</i> (LN650104), <i>glnII</i> (LN650146), <i>dnaK</i> (LN650125), <i>gyrB</i> (LN650209), <i>rpoB</i> (LN650188) and <i>nodA</i> (LN650251).
description: Published as part of <i>Avontuur, Juanita R., Palmer, Marike, Beukes, Chrizelle W., Chan, Wai Y., Tasiya, Taponeswa, Zyl, Elritha van, Coetzee, Martin P. A., Stepkowski, Tomasz, Venter, Stephanus N. &amp; Steenkamp, Emma T., 2022, Bradyrhizobium altum sp. nov., Bradyrhizobium oropedii sp. nov. and Bradyrhizobium acaciae sp. nov. from South Africa show locally restricted and pantropical nodA phylogeographic patterns, pp. 1-13 in Molecular Phylogenetics and Evolution (107338) (107338) 167</i> on page 10, DOI: 10.1016/j.ympev.2021.107338, http://zenodo.org/record/5728354