title: Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications
Creators: name: Altincekic, Nadide
name: Korn, Sophie Marianne
name: Qureshi, Nusrat Shahin
name: Dujardin, Marie
name: Ninot-Pedrosa, Martí
name: Abele, Rupert
name: Abi Saad, Marie Jose
name: Alfano, Caterina
name: Almeida, Fabio C. L.
name: Alshamleh, Islam
name: de Amorim, Gisele Cardoso
name: Anderson, Thomas K.
name: Anobom, Cristiane D.
name: Anorma, Chelsea
name: Bains, Jasleen Kaur
name: Bax, Adriaan
name: Blackledge, Martin
name: Blechar, Julius
name: Böckmann, Anja
name: Brigandat, Louis
name: Bula, Anna
name: Bütikofer, Matthias
name: Camacho-Zarco, Aldo R.
name: Carlomagno, Teresa
name: Caruso, Icaro Putinhon
name: Ceylan, Betül
name: Chaikuad, Apirat
name: Chu, Feixia
name: Cole, Laura
name: Crosby, Marquise G.
name: Jesus, Vanessa de
name: Dhamotharan, Karthikeyan
name: Felli, Isabella C.
name: Ferner, Jan
name: Fleischmann, Yanick
name: Fogeron, Marie-Laure
name: Fourkiotis, Nikolaos K.
name: Fuks, Christin
name: Fürtig, Boris
name: Gallo, Angelo
name: Gande, Santosh L.
name: Gerez, Juan Atilio
name: Ghosh, Dhiman
name: Gomes-Neto, Francisco
name: Gorbatyuk, Oksana
name: Guseva, Serafima
name: Hacker, Carolin
name: Häfner, Sabine
name: Hao, Bing
name: Hargittay, Bruno
name: Henzler-Wildman, K.
name: Hoch, Jeffrey C.
name: Hohmann, Katharina F.
name: Hutchison, Marie T.
name: Jaudzems, Kristaps
name: Jović, Katarina
name: Kaderli, Janina
name: Kalniņš, Gints
name: Kaņepe, Iveta
name: Kirchdoerfer, Robert N.
name: Kirkpatrick, John
name: Knapp, Stefan
name: Krishnathas, Robin
name: Kutz, Felicitas
name: Lage, Susanne zur
name: Lambertz, Roderick
name: Lang, Andras
name: Laurents, Douglas
name: Lecoq, Lauriane
name: Linhard, Verena
name: Löhr, Frank
name: Malki, Anas
name: Bessa, Luiza Mamigonian
name: Martin, Rachel W.
name: Matzel, Tobias
name: Maurin, Damien
name: McNutt, Seth W.
name: Mebus-Antunes, Nathane Cunha
name: Meier, Beat H.
name: Meiser, Nathalie
name: Mompeán, Miguel
name: Monaca, Elisa
name: Montserret, Roland
name: Mariño Perez, Laura
name: Moser, Celine
name: Muhle-Goll, Claudia
name: Neves-Martins, Thais Cristtina
name: Ni, Xiamonin
name: Norton-Baker, Brenna
name: Pierattelli, Roberta
name: Pontoriero, Letizia
name: Pustovalova, Yulia
name: Ohlenschläger, Oliver
name: Orts, Julien
name: Da Poian, Andrea T.
name: Pyper, Dennis J.
name: Richter, Christian
name: Riek, Roland
name: Rienstra, Chad M.
name: Robertson, Angus
name: Pinheiro, Anderson S.
name: Sabbatella, Raffaele
name: Salvi, Nicola
name: Saxena, Krishna
name: Schulte, Linda
name: Schiavina, Marco
name: Schwalbe, Harald
name: Silber, Mara
name: da Silva Almeida, Marcius
name: Sprague-Piercy, Marc A.
name: Spyroulias, Georgios A.
name: Sreeramulu, Sridhar
name: Tants, Jan-Niklas
name: Tārs, Kaspars
name: Torres, Felix
name: Töws, Sabrina
name: Treviño, Miguel Á.
name: Trucks, Sven
name: Tsika, Aikaterini C.
name: Varga, Krisztina
name: Wang, Ying
name: Weber, Marco E.
name: Weigand, Julia E.
name: Wiedemann, Christoph
name: Wirmer-Bartoschek, Julia
name: Wirtz Martin, Maria Alexandra
name: Zehnder, Johannes
name: Hengesbach, Martin
name: Schlundt, Andreas
Publisher: name: Frontiers Media S.A.
Year: 2022

description: The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in COVID19-NMR and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential basis for further work, including macromolecular interaction studies and high-throughput drug screening. Here, we present the extensive catalog of a holistic SARS-CoV-2 protein preparation approach based on the consortium’s collective efforts. We provide protocols for the large-scale production of more than 80% of all SARS-CoV-2 proteins or essential parts of them. Several of the proteins were produced in more than one laboratory, demonstrating the high interoperability between NMR groups worldwide. For the majority of proteins, we can produce isotope-labeled samples of HSQC-grade. Together with several NMR chemical shift assignments made publicly available on covid19-nmr.com, we here provide highly valuable resources for the production of SARS-CoV-2 proteins in isotope-labeled form.