Biofunctional quantum dots as fluorescence probe for cell-specific targeting
Creators: name: Ag, Didem
name: Bongartz, Rebecca
name: Dogan, Leyla Eral
name: Seleci, Muharrem
name: Walter, Johanna-Gabriela
name: Demirkol, Dilek Odaci
name: Stahl, Frank
name: Ozcelik, Serdar
name: Timur, Suna
name: Scheper, Thomas
Publisher: name: Amsterdam : Elsevier
Year: 2014
Subjects: subject: Anti-HER2
subject: Bioconjugation
subject: Cell specific targeting
subject: Imaging
subject: Quantum dots
subject: Electrophoresis
subject: Fluorescence
subject: Fluorescence microscopy
subject: Imaging techniques
subject: Semiconductor quantum dots
subject: Ultraviolet visible spectroscopy
subject: X ray diffraction
subject: Bio-conjugation
subject: Cellular localization
subject: Epidermal growth factor receptor 2
subject: Fluorescence probes
subject: Gel electrophoresis
subject: Inhibitory concentration
subject: Lethal concentration
subject: Antibodies
subject: 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide
subject: cadmium
subject: epidermal growth factor receptor 2
subject: epidermal growth factor receptor 2 antibody
subject: quantum dot
subject: receptor antibody
subject: sulfur
subject: tellurium
subject: thioglycolic acid
subject: unclassified drug
subject: animal cell
subject: cancer cell
subject: cancer diagnosis
subject: cell assay
subject: cell labeling
subject: cell specificity
subject: cell surface
subject: cellular distribution
subject: controlled study
subject: drug concentration
subject: drug conjugation
subject: drug cytotoxicity
subject: drug design
subject: drug solubility
subject: drug synthesis
subject: electron microscopy
subject: flow cytometry
subject: fluorescence imaging
subject: fluorescence microscopy
subject: human cell
subject: hydrodynamics
subject: internalization
subject: lung cancer
subject: mouse
subject: no observable adverse effect concentration
subject: nonhuman
subject: priority journal
subject: protein expression
subject: target cell
subject: total lethal concentration
subject: ultraviolet spectroscopy
subject: Cadmium Compounds
subject: Cell Death
subject: Cell Membrane
subject: Cell Survival
subject: Cells
subject: Fluorescent Dyes
subject: Light
subject: Mice
subject: Microscopy, Fluorescence
subject: NIH 3T3 Cells
subject: Receptor, erbB-2
subject: Reproducibility of Results
subject: Tellurium
subject: Thioglycolates
subject: X-Ray Diffraction
subject: Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie

description: We describe here the synthesis, characterization, bioconjugation, and application of water-soluble thioglycolic acid TGA-capped CdTe/CdS quantum dots (TGA-QDs) for targeted cellular imaging. Anti-human epidermal growth factor receptor 2 (HER2) antibodies were conjugated to TGA-QDs to target HER2-overexpressing cancer cells. TGA-QDs and TGA-QDs/anti-HER2 bioconjugates were characterized by fluorescence and UV-Vis spectroscopy, X-ray diffraction (XRD), hydrodynamic sizing, electron microscopy, and gel electrophoresis. TGA-QDs and TGA-QDs/anti-HER2 were incubated with cells to examine cytotoxicity, targeting efficiency, and cellular localization. The cytotoxicity of particles was measured using an MTT assay and the no observable adverse effect concentration (NOAEC), 50% inhibitory concentration (IC50), and total lethal concentration (TLC) were calculated. To evaluate localization and targeting efficiency of TGA-QDs with or without antibodies, fluorescence microscopy and flow cytometry were performed. Our results indicate that antibody-conjugated TGA-QDs are well-suited for targeted cellular imaging studies.