cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Light front QED, Stueckelberg field and Infrared divergence
Creators: name: Govindarajan, T. R.
name: More, Jai D.
name: Ramadevi, P.
Publisher: name: arXiv
Year: 2019
Subjects: subject: High Energy Physics - Theory (hep-th)
subject: High Energy Physics - Phenomenology (hep-ph)
subject: FOS: Physical sciences

description: Stueckelberg mechanism introduces a scalar field, known as Stueckelberg field, so that gauge symmetry is preserved in the massive abelian gauge theory. In this work, we show that the role of the Stueckelberg field is similar to the Kulish and Faddeev coherent state approach to handle infrared (IR) divergences. We expect that the light-front quantum electrodynamics (LFQED) with Stueckelberg field must be IR finite in the massless limit of the gauge boson. We have explicitly shown the cancellation of IR divergences in the relevant diagrams contributing to self-energy and vertex correction at leading order.
description: 12 pages, 2 figures, references added
The MKK2 Pathway Mediates Cold and Salt Stress Signaling in Arabidopsis
Creators: name: Teige, Markus
name: Dóczi, Róbert
name: Shinozaki, Kazuo
name: Scheikl, Elisabeth
name: Ichimura, Kazuya
name: Dangl, Jeffery L.
name: Hirt, Heribert
name: Eulgem, Thomas
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2004

description: AbstractThe Arabidopsis mitogen-activated protein kinase (MAPK) kinase 2 (MKK2) and the downstream MAPKs MPK4 and MPK6 were isolated by functional complementation of osmosensitive yeast mutants. In Arabidopsis protoplasts, MKK2 was specifically activated by cold and salt stress and by the stress-induced MAPK kinase kinase MEKK1. Yeast two-hybrid, in vitro, and in vivo protein kinase assays revealed that MKK2 directly targets MPK4 and MPK6. Accordingly, plants overexpressing MKK2 exhibited constitutive MPK4 and MPK6 activity, constitutively upregulated expression of stress-induced marker genes, and increased freezing and salt tolerance. In contrast, mkk2 null plants were impaired in MPK4 and MPK6 activation and were hypersensitive to salt and cold stress. Full genome transcriptome analysis of MKK2-overexpressing plants demonstrated altered expression of 152 genes involved in transcriptional regulation, signal transduction, cellular defense, and stress metabolism. These data identify a MAP kinase signaling cascade mediating cold and salt stress tolerance in plants.
Higgs Bundles for M-theory on $G_2$-Manifolds
Creators: name: Braun, Andreas P.
name: Cizel, Sebastjan
name: Hubner, Max
name: Schafer-Nameki, Sakura
Publisher: name: arXiv
Year: 2018
Subjects: subject: High Energy Physics - Theory (hep-th)
subject: Differential Geometry (math.DG)
subject: FOS: Physical sciences
subject: FOS: Mathematics

description: M-theory compactified on $G_2$-holonomy manifolds results in 4d $\mathcal{N}=1$ supersymmetric gauge theories coupled to gravity. In this paper we focus on the gauge sector of such compactifications by studying the Higgs bundle obtained from a partially twisted 7d super Yang-Mills theory on a supersymmetric three-cycle $M_3$. We derive the BPS equations and find the massless spectrum for both abelian and non-abelian gauge groups in 4d. The mathematical tool that allows us to determine the spectrum is Morse theory, and more generally Morse-Bott theory. The latter generalization allows us to make contact with twisted connected sum (TCS) $G_2$-manifolds, which form the largest class of examples of compact $G_2$-manifolds. M-theory on TCS $G_2$-manifolds is known to result in a non-chiral 4d spectrum. We determine the Higgs bundle for this class of $G_2$-manifolds and provide a prescription for how to engineer singular transitions to models that have chiral matter in 4d.
description: 82 pages, 16 figures; v2: ref added, typos fixed
Sterols sense swelling in lipid bilayers
Creators: name: Richter, F.
name: Finegold, L.
name: Rapp, G.
Publisher: name: arXiv
Year: 1998
Subjects: subject: Soft Condensed Matter (cond-mat.soft)
subject: FOS: Physical sciences

description: In the mimetic membrane system of phosphatidylcholine bilayers, thickening (pre-critical behavior, anomalous swelling) of the bilayers is observed, in the vicinity of the main transition, which is non-linear with temperature. The sterols cholesterol and androsten are used as sensors in a time-resolved simultaneous small- and wide angle x-ray diffraction study to investigate the cause of the thickening. We observe precritical behavior in the pure lipid system, as well as with sterol concentrations less than 15%. To describe the precritical behavior we introduce a theory of precritical phenomena.The good temperature resolution of the data shows that a theory of the influence of fluctuations needs modification. The main cause of the critical behavior appears to be a changing hydration of the bilayer.
description: 11 pages, 7 ps figures included, to appear in Phys.Rev.E
Supersymmetry anomalies in $\mathcal{N}=1$ conformal supergravity
Creators: name: Papadimitriou, Ioannis
Publisher: name: arXiv
Year: 2019
Subjects: subject: High Energy Physics - Theory (hep-th)
subject: FOS: Physical sciences

description: We solve the Wess-Zumino consistency conditions of $\mathcal{N}=1$ off-shell conformal supergravity in four dimensions and determine the general form of the superconformal anomalies for arbitrary $a$ and $c$ anomaly coefficients to leading non trivial order in the gravitino. Besides the well known Weyl and $R$-symmetry anomalies, we compute explicitly the fermionic $\mathcal{Q}$- and $\mathcal{S}$-supersymmetry anomalies. In particular, we show that $\mathcal{Q}$-supersymmetry is anomalous if and only if $R$-symmetry is anomalous. The $\mathcal{Q}$- and $\mathcal{S}$-supersymmetry anomalies give rise to an anomalous supersymmetry transformation for the supercurrent on curved backgrounds admitting Killing spinors, resulting in a deformed rigid supersymmetry algebra. Our results may have implications for supersymmetric localization and supersymmetry phenomenology. Analogous results are expected to hold in dimensions two and six and for other supergravity theories. The present analysis of the Wess-Zumino consistency conditions reproduces the holographic result of arxiv:1703.04299 and generalizes it to arbitrary $a$ and $c$ anomaly coefficients.
description: 13+13 pages; v2: minor corrections and improvements; references added; v3: further minor typos corrected; version published in JHEP
Histone H3 and H4 Ubiquitylation by the CUL4-DDB-ROC1 Ubiquitin Ligase Facilitates Cellular Response to DNA Damage
Creators: name: Erdjument-Bromage, Hediye
name: Zhai, Ling
name: Tempst, Paul
name: Xiong, Yue
name: Xu, Jun
name: Jackson, Sarah
name: Wang, Hengbin
name: Joo, Heui-Yun
name: Zhang, Yi
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2006

description: Posttranslational histone modifications play important roles in transcription and other chromatin-based processes. Compared to acetylation, methylation, and phosphorylation, very little is known about the function of histone ubiquitylation. Here, we report the purification and functional characterization of a histone H3 and H4 ubiquitin ligase complex, CUL4-DDB-ROC1. We demonstrate that CUL4-DDB-ROC1-mediated H3 and H4 ubiquitylation occurs both in vitro and in vivo. Importantly, CUL4-DDB-ROC1-mediated H3 and H4 ubiquitylation is regulated by UV irradiation. Reduction of histone H3 and H4 ubiquitylation by knockdown of CUL4A impairs recruitment of the repair protein XPC to the damaged foci and inhibits the repair process. Biochemical studies indicate that CUL4-DDB-ROC1-mediated histone ubiquitylation weakens the interaction between histones and DNA and facilitates the recruitment of repair proteins to damaged DNA. Thus, our studies uncover CUL4-DDB-ROC1 as a histone ubiquitin ligase and demonstrate that histone H3 and H4 ubiquitylation participates in the cellular response to DNA damage.
A Genome-wide RNA Interference Screen Reveals that Variant Histones Are Necessary for Replication-Dependent Histone Pre-mRNA Processing
Creators: name: Godfrey, Ashley C.
name: Salzler, Harmony R.
name: Duronio, Robert J.
name: Wagner, Eric J.
name: Burch, Brandon D.
name: Marzluff, William F.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2007

description: Metazoan replication-dependent histone mRNAs are not polyadenylated and instead end in a conserved stem loop that is the cis element responsible for coordinate posttranscriptional regulation of these mRNAs. Using biochemical approaches, only a limited number of factors required for cleavage of histone pre-mRNA have been identified. We therefore performed a genome-wide RNA interference screen in Drosophila cells using a GFP reporter that is expressed only when histone pre-mRNA processing is disrupted. Four of the 24 genes identified encode proteins also necessary for cleavage/polyadenylation, indicating mechanistic conservation in formation of different mRNA 3' ends. We also unexpectedly identified the histone variants H2Av and H3.3A/B. In H2Av mutant cells, U7 snRNP remains active but fails to accumulate at the histone locus, suggesting there is a regulatory pathway that coordinates the production of variant and canonical histones that acts via localization of essential histone pre-mRNA processing factors.
Endogenous Formaldehyde Is a Hematopoietic Stem Cell Genotoxin and Metabolic Carcinogen
Creators: name: Patel, Ketan J.
name: Pontel, Lucas B.
name: Arends, Mark J.
name: Chandrasekaran, Gayathri
name: Yu, Rui
name: Garaycoechea, Juan I.
name: Wei, Wei
name: Broecker, Verena
name: Liu, Limin
name: Burgos-Barragan, Guillermo
name: Rosado, Ivan V.
name: Crossan, Gerry P.
name: Swenberg, James A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2015

description: Endogenous formaldehyde is produced by numerous biochemical pathways fundamental to life, and it can crosslink both DNA and proteins. However, the consequences of its accumulation are unclear. Here we show that endogenous formaldehyde is removed by the enzyme alcohol dehydrogenase 5 (ADH5/GSNOR), and Adh5−/− mice therefore accumulate formaldehyde adducts in DNA. The repair of this damage is mediated by FANCD2, a DNA crosslink repair protein. Adh5−/−Fancd2−/− mice reveal an essential requirement for these protection mechanisms in hematopoietic stem cells (HSCs), leading to their depletion and precipitating bone marrow failure. More widespread formaldehyde-induced DNA damage also causes karyomegaly and dysfunction of hepatocytes and nephrons. Bone marrow transplantation not only rescued hematopoiesis but, surprisingly, also preserved nephron function. Nevertheless, all of these animals eventually developed fatal malignancies. Formaldehyde is therefore an important source of endogenous DNA damage that is counteracted in mammals by a conserved protection mechanism.
CPA density of states and conductivity in a double-exchange system containing impurities
Creators: name: Auslender, Mark
name: Kogan, Eugene
Publisher: name: arXiv
Year: 2000
Subjects: subject: Condensed Matter (cond-mat)
subject: FOS: Physical sciences

description: We study density of states and conductivity of the doped double-exchange system, treating interaction of charge carriers both with the localized spins and with the impurities in the coherent potential approximation. It is shown that under appropriate conditions there is a gap between the conduction band and the impurity band in paramagnetic phase, while the density of states is gapless in ferromagnetic phase. This can explain metal-insulator transition frequently observed in manganites and magnetic semiconductors. Activated conductivity in the insulator phase is numerically calculated.
description: 5 pages, 2 eps figures, LaTex2e, EPJ macro package (style files included). The calculations done for realistic model predict that a gap appears between the conduction band and the impurity band in paramagnetic phase, while the density of states is gapless in ferromagnetic phase
Additional file 1 of Effect of nephrostomy sheath size on renal pelvic pressure during endoscopic combined intrarenal surgery: artificial kidney model study
Creators: name: Iguchi, Takashi
name: Yamashita, Shimpei
name: Kohjimoto, Yasuo
name: Tanioku, Tadashi
name: Kawamata, Tomoyuki
name: Hara, Isao
Publisher: name: figshare
Year: 2024
Subjects: subject: Space Science
subject: Medicine
subject: Biotechnology
subject: Environmental Sciences not elsewhere classified
subject: Ecology
subject: FOS: Biological sciences
subject: Biological Sciences not elsewhere classified
subject: Marine Biology
subject: Cancer

description: Supplementary Material 1
Recommendations for the use of sapropterin in phenylketonuria
Creators: name: DeFouw, Kari
name: Skrabal, Jill
name: Jurecki, Elaina
name: Ernst, Sharon
name: Gillis, Jane
name: McClure, Julie
name: Shediac, Renée
name: Bausell, Heather
name: Cunningham, Amy
name: McCune, Helen
name: Prasad, Suyash
name: Wessel, Ann
name: Cederbaum, Stephen
name: Brown, Mary
name: O'Steen, Donna
name: Pender, Amy
name: Chapman, Maggie
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2012

description: Phenylketonuria (PKU) is an inherited disorder of phenylalanine (Phe) metabolism. Until recently, the only treatment for PKU was a Phe-restricted diet. Increasing evidence of suboptimal outcomes in diet-treated individuals, inconsistent PKU management practices, and the recent availability of tetrahydrobiopterin (BH(4)) therapy have fueled the need for new management and treatment recommendations for this metabolic disorder. BH(4), now available as sapropterin dihydrochloride (sapropterin), may offer the potential for improved metabolic control as well as enhanced dietary Phe tolerance in some PKU patients. A group of metabolic dietitians from North America convened in June 2011 to draft recommendations for the use of sapropterin therapy in PKU. Physicians with extensive experience in PKU management were invited at a later date to contribute to the development of these recommendations. Based on extensive clinical experience and current evidence, the present recommendations provide guidance from patient selection and determination of sapropterin response to the long-term management of patients on sapropterin therapy. Target Phe levels, nutritional adequacy, neurocognitive screening and adherence to treatment are addressed to optimize patient outcomes.
Magnetic Phase Diagram and Metal-Insulator Transition of NiS2-xSex
Creators: name: Matsuura, M.
name: Hiraka, H.
name: Yamada, K.
name: Endoh, Y.
Publisher: name: arXiv
Year: 2000
Subjects: subject: Strongly Correlated Electrons (cond-mat.str-el)
subject: FOS: Physical sciences

description: Magnetic phase diagram of NiS2-xSex has been reexamined by systematic studies of electrical resistivity, uniform magnetic susceptibility and neutron diffraction using single crystals grown by a chemical transport method. The electrical resistivity and the uniform magnetic susceptibility exhibit the same feature of temperature dependence over a wide Se concentration. A distinct first order metal-insulator (M-I) transition accompanied by a volume change was observed only in the antiferromagnetic ordered phase for 0.50
description: 6 pages, 8 figures, corrected EPS file
Microvelia tshingandana Linnavuori & Weber 1974
Creators: name: Kment, Petr
name: Kolínová, Zdislava
Publisher: name: Zenodo
Year: 2013
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hemiptera
Veliidae
Microvelia
Microvelia tshingandana

description: Microvelia tshingandana Linnavuori & Weber, 1974
 Microvelia tshingandana Linnavuori & Weber, 1974: 331−332, Figs 1a− e (original description).
One paratype is deposited in NMPC:
PARATYPE (J apter.): ‘Zentral-Afrika [hw] // Tshinganda - Qache / 2450 m 5.4.72 / Statzner leg. [hw] // J [p] // Paratypus / Microvelia / tshingandana / Lv. et Hb. [hw, red label]’.
 Current status. Valid species, not attributed to any subgenus.
description: Published as part of Kment, Petr & Kolínová, Zdislava, 2013, Catalogue of type specimens of true bugs (Hemiptera: Heteroptera) deposited in the National Museum, Prague, Czech Republic, pp. 821-890 in Acta Entomologica Musei Nationalis Pragae 53 (2) on page 847, DOI: 10.5281/zenodo.5740731
Online Human Gesture Recognition using Recurrent Neural Networks and Wearable Sensors
Creators: name: Carfi, Alessandro
name: Motolese, Carola
name: Bruno, Barbara
name: Mastrogiovanni, Fulvio
Publisher: name: arXiv
Year: 2018
Subjects: subject: Robotics (cs.RO)
subject: FOS: Computer and information sciences

description: Gestures are a natural communication modality for humans. The ability to interpret gestures is fundamental for robots aiming to naturally interact with humans. Wearable sensors are promising to monitor human activity, in particular the usage of triaxial accelerometers for gesture recognition have been explored. Despite this, the state of the art presents lack of systems for reliable online gesture recognition using accelerometer data. The article proposes SLOTH, an architecture for online gesture recognition, based on a wearable triaxial accelerometer, a Recurrent Neural Network (RNN) probabilistic classifier and a procedure for continuous gesture detection, relying on modelling gesture probabilities, that guarantees (i) good recognition results in terms of precision and recall, (ii) immediate system reactivity.
Open curation of knowledge on biodiversity: An introduction to Wikidata
Creators: name: Taraborelli, Dario
Publisher: name: figshare
Year: 2019
Subjects: subject: 50202 Conservation and Biodiversity
subject: FOS: Biological sciences
subject: 170203 Knowledge Representation and Machine Learning
subject: FOS: Psychology
subject: 80703 Human Information Behaviour
subject: FOS: Media and communications
subject: 80610 Information Systems Organisation
subject: FOS: Computer and information sciences

description: Slides from my talk on Wikidata as infrastructure to represent knowledge on biodiversity at the Smithsonian Tropical Research Institute (STRI) in Gamboa, Panama (February 27, 2019).
 Open curation of knowledge on biodiversity: An introduction to Wikidata.
In recent years, a number of research institutions, cultural organizations, citizen science contributors and open source developers have been curating a live, growing body of structured knowledge on biodiversity in Wikidata—the open knowledge base that anyone can edit and Wikipedia’s fastest growing sister project. As of today, there are 2.6M taxonomical entries and over 2M statements referencing GBIF identifiers in Wikidata. Wikidata also includes information and metadata about tens of millions of scientific papers, researchers and scholarly institutions. In this talk I’ll give a hands-on introduction to Wikidata and show how Wikimedia projects more generally can amplify the output of research communities and institutions, engage the general public, and help maximize the reach and reuse of knowledge and data they produce.
Microvelia (Microvelia) vilhenai Hoberlandt 1951
Creators: name: Kment, Petr
name: Kolínová, Zdislava
Publisher: name: Zenodo
Year: 2013
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hemiptera
Veliidae
Microvelia
Microvelia vilhenai

description: Microvelia (Microvelia) vilhenai Hoberlandt, 1951
 Microvelia (Microvelia) vilhenai Hoberlandt, 1951a: 31–33, Figs 83–91 (original description).
Seven paratypes are deposited in NMPC:
PARATYPES (4 JJ apter.): ‘Dundo, Lunda / Angola [p] VI. 49 [hw] / A. B. Machado lgt. [p] // Ang. [p] No 1525.12 [hw] / coll.A.B.Machado [p] // Paratypus [p, red label] // Paratypus [p] / Microvelia / vilhenai / sp. n. J [hw] / Det. L. Hoberlandt, 19[p]50 [hw. red label]’.
PARATYPES (3 ♀♀ apter.): ‘Dundo, Lunda / Angola [p] VI. 49 [hw] / A. B. Machado lgt. [p] // Ang. [p] No 1525.12 [hw] / coll.A.B.Machado [p] // Paratypus [p, red label] // Paratypus [p] / Microvelia / vilhenai / sp. n. ♀ [hw] / Det. L. Hoberlandt, 19[p]50 [hw. red label]’.
 Note. Collection also includes two slides, one with antenna, fore, middle and hind leg, the other one with male genitalia, of an additional paratype specimen.
 Current status. Valid species (see POISSON 1957).
description: Published as part of Kment, Petr & Kolínová, Zdislava, 2013, Catalogue of type specimens of true bugs (Hemiptera: Heteroptera) deposited in the National Museum, Prague, Czech Republic, pp. 821-890 in Acta Entomologica Musei Nationalis Pragae 53 (2) on page 847, DOI: 10.5281/zenodo.5740731
Development of a Competitive PCR Method for Physical Titration of Recombinant EBV Vector in a Helper-Dependent Packaging System
Creators: name: Wang, Jianlong
name: Vos, Jean-Michel H.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2001

description: AbstractEpstein–Barr virus (EBV) is a γ-herpesvirus with B lymphotropism and a double-stranded DNA genome of 172 kb that is episomally maintained in permissive cells during latency. EBV-based vectors containing minimal cis elements for replication, amplification, and helper-dependent packaging in a producer cell line HH514 have been developed to deliver therapeutic/suicide transgenes as infectious viral particles (miniEBV) to EBV-transformed B lymphoblastoid cells or B lymphoma cells. A quantitative, competitive PCR-based assay was developed to determine the relative packaging efficiencies of miniEBV and helper P3HR1 coproduced in HH514 cells. This provides a rapid and accurate quantitation of the physical titer of the virus preparation, which helps preserve the biological titer of the virus preparation and increase the efficiency of transgene delivery by miniEBV infection. In addition, it provides a sensitive and accurate way to evaluate future development of a helper-free packaging system by detecting any possible helper virus contamination.
Electron Correlations in Molecular Systems
Creators: name: Bulka, Bogdan R.
Publisher: name: arXiv
Year: 1998
Subjects: subject: Materials Science (cond-mat.mtrl-sci)
subject: Strongly Correlated Electrons (cond-mat.str-el)
subject: FOS: Physical sciences

description: A short review of correlated electrons in molecular systems has been performed. Main attention has been focussed on ET salts, which are the d=2 systems. They show the Mott transition in high temperatures and the transition from the antiferromagnetic to the superconducting phase in low temperatures, under a (chemical) pressure. Physical properties (the electrical resistivity, the specific heat, the magnetic susceptibility, the photoemission spectra, the optical conductivity) of ET salts have been compared with those ones in other strongly correlated systems. The optical conductivity is described in the framework of the Hubbard model, with a low frequency peak as an evidence for the Abrikosov-Suhl resonance.
description: 9 pages, 4 figures, lecture given at the Conf. Molecular Crystals '98, Gdansk, Poland, September 9-12, 1998, to appear in Molecular Physics Reports
КОРРУРИЯГА    ҚАРШИ   КУРАШИШ    УСУЛЛАРИ
Creators: name: Ҳамроқулова Дурдона Шерали қизи Термиз давлат университети Юридик факултети 1-босқич талабаси
Publisher: name: Zenodo
Year: 2024

description: Ушбу мақолада коррупсия ва унинг салбий оқибатлари, айнан коррупсия натижасида вужудга келаётган ҳолатларга қарши курашиш, коррупсияни олдини олишда, хусусан, унга қарши курашишда қонунчилик нуқтаи назаридан ҳамда халқаро тажрибадан келиб чиқиб ёнадҳувлар ҳақида сўз боради.

                  Калит сўзлар: коррупсия қарши кураш, коррупсиянинг оқибатлари, халқаро тажриба, жазо тизими, ички назорат, ташқи назорат, жамоатчилик назорат, сайлов орқали курашиш
Neurological Correction of Lysosomal Storage in a Mucopolysaccharidosis IIIB Mouse Model by Adeno-associated Virus-Mediated Gene Delivery
Creators: name: Muenzer, Joseph
name: McCown, Thomas J.
name: Fu, Haiyan
name: Picornell, Yoana J.
name: Samulski, Richard J.
name: Fletcher, David
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2002

description: AbstractMucopolysaccharidosis (MPS) IIIB is characterized by mild somatic features and severe neurological diseases leading to premature death. No definite treatment is available for MPS IIIB patients. We constructed two recombinant adeno-associated virus (rAAV) vectors containing the human α-N-acetylglucosaminidase (NaGlu) cDNA driven by either a CMV or a neuron-specific enolase (NSE) promoter. In vitro, these rAAV vectors mediated efficient expression of recombinant NaGlu in human MPS IIIB fibroblasts and mouse MPS IIIB somatic and brain primary cell cultures. The secreted rNaGlu was taken up by both human and mouse MPS IIIB cells in culture and degraded the accumulated glycosaminoglycans (GAG). A direct microinjection (107 viral particles, 1 μl/10 minutes per injection) of vectors containing the NSE promoter resulted in long-term (6 months, the duration of the experiments) expression of rNaGlu in multiple brain structures/areas of adult MPS IIIB mice. Consistent with previous studies, the main target cells were neurons. However, while vector typically transduced an area of 400–500 μm surrounding the infusion sites, the correction of GAG storage involved neurons of a much broader area (1.5 mm) in a 6-month duration of experiments. These results provide a basis for the development of a treatment for neurological disease in MPS IIIB patients using AAV vectors.
Phenotype correction of fanconi anemia group a hematopoietic stem cells using lentiviral vector
Creators: name: Walsh, Christopher E
name: Ramezani, Ali
name: Ebell, Wolfram
name: Hawley, Robert G
name: Arnold, Larry W
name: Yamada, Kaoru
name: Arwert, Fre
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2003

description: AbstractFanconi anemia (FA) is an autosomal recessive disease characterized by progressive bone marrow failure due to defective stem cell function. FA patients' cells are hypersensitive to DNA cross-linking agents such as mitomycin C (MMC), exposure to which results in cytogenetic aberrations and cell death. To date Moloney murine leukemia virus vectors have been used in clinical gene therapy. Recently, third-generation lentiviral vectors based on the HIV-1 genome have been developed for efficient gene transfer to hematopoietic stem cells. We generated a self-inactivating lentiviral vector expressing the FA group A cDNA driven by the murine stem cell virus U3 LTR promoter and used the vector to transduce side-population (SP) cells isolated from bone marrow of Fanconi anemia group A (Fanca) knockout mice. One thousand transduced SP cells reconstituted the bone marrow of sublethally irradiated Fanca recipient mice. Phenotype correction was demonstrated by stable hematopoiesis following MMC challenge. Using real-time PCR, one proviral vector DNA copy per cell was detected in all lineage-committed cells in the peripheral blood of both primary and secondary recipients. Our results suggest that the lentiviral vector transduces stem cells capable of self-renewal and long-term hematopoiesis in vivo and is potentially useful for clinical gene therapy of FA hematopoietic cells.
Recombinant adeno-associated virus serotype 4 mediates unique and exclusive long-term transduction of retinal pigmented epithelium in rat, dog, and nonhuman primate after subretinal delivery
Creators: name: Chenuaud, Pierre
name: Moullier, Philippe
name: Rabinowitz, Joseph
name: Briot, Delphine
name: Folliot, Sébastien
name: Rolling, Fabienne
name: Weber, Michel
name: Samulski, Jude
name: Conrath, Hervé
name: Provost, Nathalie
name: Chérel, Yan
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2003

description: AbstractWe previously described chimeric recombinant adeno-associated virus (rAAV) vectors 2/4 and 2/5 as the most efficient vectors in rat retina. We now characterize these two vectors carrying the CMV.gfp genome following subretinal injection in the Wistar rat, beagle dog, and cynomolgus macaque. Both serotypes displayed stable GFP expression for the duration of the experiment (6 months) in all three animal models. Similar to the AAV-2 serotype, AAV-2/5 transduced both RPE and photoreceptor cells, with higher level of transduction in photoreceptors, whereas rAAV-2/4 transduction was unambiguously restricted to RPE cells. This unique specificity found conserved among all three species makes AAV-2/4-derived vectors attractive for retinal diseases originating in RPE such as Leber congenital amaurosis (RPE65) or retinitis pigmentosa due to a mutated mertk gene. To provide further important preclinical data, vector shedding was monitored by PCR in various biological fluids for 2 months post-rAAV administration. Following rAAV-2/4 and -5 subretinal delivery in dogs (n = 6) and in nonhuman primates (n = 2), vector genome was found in lacrymal and nasal fluids for up to 3–4 days and in the serum for up to 15–20 days. Overall, these findings will have a practical impact on the development of future gene therapy trials of retinal diseases.
Recommendations for Using ORCID in Repositories
Creators: name: Krznarich, Liz
Publisher: name: ORCID
Year: 2019
Subjects: subject: Information Systems
subject: FOS: Computer and information sciences

description: We're delighted to share the final version of our ORCID in Repositories Task Force recommendations, which we hope will be widely adopted by repository platform providers and managers.
Self-complementary adeno-associated virus serotype 2 vector: global distribution and broad dispersion of AAV-mediated transgene expression in mouse brain
Creators: name: Zeng, Xinhua
name: Samulski, Richard J
name: Sifford, Jerillyn
name: Muenzer, Joseph
name: McCarty, Douglas M
name: Fu, Haiyan
name: Breese, George
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2003

description: AbstractThe blood–brain barrier is the main obstacle to efficient delivery of therapeutic reagents, including viral vectors, into the central nervous system (CNS) for treating global CNS diseases. In this study, the effects of mannitol infusions on global brain gene expression of a novel AAV vector were examined after intravenous (iv) or intracisternal injection. Initially, a self-complementary adeno-associated virus serotype 2 vector (scAAV) was compared to traditional single-stranded AAV2 vector for reporter gene expression in the brain of adult mice with or without pretreatment of an iv mannitol infusion. One to two months postinjection, analysis of vector-transduced green fluorescent protein (GFP) expression in the brain revealed that vector delivery to the CNS via iv injection required pretreatment with mannitol. This expression was observed only when scAAV vectors were used. Using these conditions, transgene expression was observed in various neurons and glial cells throughout the brain. The peripherally administered scAAV vectors also transduced the cells in multiple somatic tissues with efficient expression in liver (20–30% of hepatocytes), but was less efficient in other somatic tissues. Intracisternal injection of scAAV vector produced a broad and intense transgene expression in both neurons and glial cells in the CNS of injected mice ranging from the olfactory area to the brain stem and spinal cord. More than 50% of the Purkinje cells in the cerebellum expressed GFP. Intravenous infusion of mannitol before intracisternal injection of the scAAV vector enhanced the dispersion of the vector in the CNS. Further optimization of these steps combining peripheral and intracisternal scAAV gene delivery should facilitate the development of treatments for global CNS diseases, especially diseases involving both the somatic system and the CNS, such as lysosomal storage disorders.
The Charge Ordered State from Weak to Strong Coupling
Creators: name: Ciuchi, S.
name: de Pasquale, F.
Publisher: name: arXiv
Year: 1998
Subjects: subject: Strongly Correlated Electrons (cond-mat.str-el)
subject: FOS: Physical sciences

description: We apply the Dynamical Mean Field Theory to the problem of charge ordering. In the normal state as well as in the Charge Ordered (CO) state the existence of polarons, i.e. electrons strongly coupled to local lattice deformation, is associated to the qualitative properties of the Lattice Polarization Distribution Function (LPDF). At intermediate and strong coupling a CO state characterized by a certain amount of thermally activated defects arise from the spatial ordering of preexisting randomly distributed polarons. Properties of this particular CO state gives a qualitative understanding of the low frequency behavior of optical conductivity of $Ni$ perovskites.
description: 4 pages, 3 figures, to be published in J. of Superconductivity (proceedings Stripes 98)
Exact solutions of the Lawrence-Doniach model for layered superconductors
Creators: name: Kuplevakhsky, Sergey V.
Publisher: name: arXiv
Year: 2000
Subjects: subject: Superconductivity (cond-mat.supr-con)
subject: FOS: Physical sciences

description: We solve the problem of exact minimization of the Lawrence-Doniach (LD) free-energy functional in parallel magnetic fields. We consider both the infinite in the layering direction case (the infinite LD model) and the finite one (the finite LD model). We prove that, contrary to a prevailing view, the infinite LD model does not admit solutions in the form of isolated Josephson vortices. For the infinite LD model, we derive a closed, self-consistent system of mean-field equations involving only two variables. Exact solutions to these equations prove simultaneous penetration of Josephson vortices into all the barriers, accompanied by oscillations and jumps of the magnetization, and yield a completely new expression for the lower critical field. Moreover, the obtained equations allow us to make self-consistent refinements on such well-known results as the Meissner state, Fraunhofer oscillations of the critical Josephson current, the upper critical field, and the vortex solution of Theodorakis [S. Theodorakis, Phys. Rev. B, v. 42, 10172 (1990)]. Our consideration of the finite LD model illuminates the role of the boundary effect. In contrast to the infinite case, an explicit analytical solution to the Maxwell equations of the finite case does not preclude the existence of localized Josephson vortex configurations. By the use of this solution, we obtain a self-consistent description of the Meissner state. Finally, we discuss some theoretical and experimental implications.
description: 18 pages, 23 references
Pseudovelia heissi Hecher 2006
Creators: name: Kment, Petr
name: Kolínová, Zdislava
Publisher: name: Zenodo
Year: 2013
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hemiptera
Veliidae
Pseudovelia
Pseudovelia heissi

description: Pseudovelia heissi Hecher, 2006
 Pseudovelia heissi Hecher, 2006: 436, 447–455, Figs 2, 12, 33–35, 46, 49, 58 (original description, key).
Two paratypes are deposited in NMPC (exchange with Natural History Museum, Vienna):
PARATYPE (J apter.): ‘ Philippinen: Catanduanes / W Bato / Maribini Falls / 6.3.1999 / leg. H. Zettel (194) [p] // J [p] // Paratypus / Pseudovelia / heissi sp.n. / des. Hecher 2006 [p, red label]’.
PARATYPE (♀ apter.): ‘ Philippinen: Camarines Sur / 20km E Naga, 3km E Carolina / Mainit Spring (Hydro) / 20.2.1998, leg.Zettel (142) [p] // ♀ [p] // Paratypus / Pseudovelia / heissi sp.n. / des. Hecher 2006 [p, red label]’.
 Current status. Valid species: Pseudovelia (Pseudovelia) heissi Hecher, 2006.
description: Published as part of Kment, Petr & Kolínová, Zdislava, 2013, Catalogue of type specimens of true bugs (Hemiptera: Heteroptera) deposited in the National Museum, Prague, Czech Republic, pp. 821-890 in Acta Entomologica Musei Nationalis Pragae 53 (2) on page 847, DOI: 10.5281/zenodo.5740731
Differential Myocardial Gene Delivery by Recombinant Serotype-Specific Adeno-associated Viral Vectors
Creators: name: Jamieson, Stuart W.
name: Weitzman, Matthew D.
name: Samulski, R.Jude
name: Rabinowitz, Joseph E.
name: Thistlethwaite, Patricia A.
name: Lee, Darwin V.
name: Kido, Masakuni
name: Du, Lingling
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2004

description: AbstractRecombinant cross-packaging of adeno-associated virus (AAV) genome of one serotype into other AAV serotypes has the potential to optimize tissue-specific gene transduction and expression in the heart. To evaluate the role of AAV1 to 5 virion shells on AAV2 transgene transduction, we constructed hybrid vectors in which each serotype capsid coding domain was cloned into a common vector backbone containing AAV2 replication genes. Constructs were tested for expression in: (1) adult murine heart in vivo using direct injection of virus, (2) neonatal and adult murine ventricular cardiomyocytes in vitro, and (3) adult human ventricular cardiomyocytes in vitro, using green fluorescent protein (GFP) as the measurable transgene. Serotype 1 virus demonstrated the highest transduction efficiency in adult murine cardiomyocytes both in vitro and in vivo, while serotype 2 virus had the greater transduction efficiency in neonatal cardiomyocytes in vitro. Prolonged in vivo myocardial GFP expression was observed for up to 12 months using serotype 1 and 2 vectors only. In human cardiomyocytes, serotype 1 vector was superior in transduction efficiency, followed by types 2, 5, 4, and 3. These data establish a hierarchy for efficient serotype-specific vector transduction in myocardial tissue. AAV1 serotype packaging results in more efficient transduction of genes in the murine and human adult heart, compared to other AAV serotypes. Our results suggest that adult human cardiac gene therapy may be enhanced by the use of serotype 1-specific AAV vectors.
Non trivial overlap distributions at zero temperature
Creators: name: Franz, Silvio
name: Parisi, Giorgio
Publisher: name: arXiv
Year: 2000
Subjects: subject: Disordered Systems and Neural Networks (cond-mat.dis-nn)
subject: Statistical Mechanics (cond-mat.stat-mech)
subject: FOS: Physical sciences

description: We explore the consequences of Replica Symmetry Breaking at zero temperature. We introduce a repulsive coupling between a system and its unperturbed ground state. In the Replica Symmetry Breaking scenario a finite coupling induces a non trivial overlap probability distribution among the unperturbed ground state and the one in presence of the coupling. We find a closed formula for this probability for arbitrary ultrametric trees, in terms of the parameters defining the tree. The same probability is computed in numerical simulations of a simple model with many ground states, but no ultrametricity: polymers in random media in 1+1 dimension. This gives us an idea of what violation of our formula can be expected in cases when ultrametricity does not hold.
description: 9 pages, 8 figures; 3 references added, address corrected
Additional file 5 of MiR-612 regulates invadopodia of hepatocellular carcinoma by HADHA-mediated lipid reprogramming
Creators: name: Liu, Yang
name: Lu, Li-Li
name: Wen, Duo
name: Liu, Dong-Li
name: Dong, Li-Li
name: Gao, Dong-Mei
name: Bian, Xin-Yu
name: Zhou, Jian
name: Fan, Jia
name: Wu, Wei-Zhong
Publisher: name: figshare
Year: 2020
Subjects: subject: Biochemistry
subject: Space Science
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Pharmacology
subject: Evolutionary Biology
subject: Chemical Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Plant Biology
subject: Computational Biology

description: Additional file 5: Table S1. Primers sequences of PCR. Table S2. Reaction Mixes for Acetyl-Coenzyme A Assay (unit: μl). Table S3. ATP detection operation rules (unit: μl). Table S4. Reaction mixes for cholesterol quantitation assay (unit: μl).
Spin Dependence of the Critical Concentration for the Neel State of 2D Impure Heisenberg Antiferromagnets
Creators: name: Yasuda, Chitoshi
name: Oguchi, Akihide
Publisher: name: arXiv
Year: 1998
Subjects: subject: Disordered Systems and Neural Networks (cond-mat.dis-nn)
subject: Materials Science (cond-mat.mtrl-sci)
subject: FOS: Physical sciences

description: The spin-wave theory and the coherent potential approximation are applied to a spin S Heisenberg antiferromagnet with nonmagnetic impurities on square lattice. The impurity effects are taken into account by substituting S(1-x) for S and using the coherent potential approximation to the exchange interaction, where x is the impurity concentration. At T=0 for S=1/2 the critical impurity concentration xc of the Neel state is 0.303 and the percolation threshold xp is 0.500. The ground state in xc=1 the long range Neel order vanishes at xp=0.500. These results explain qualitatively the experimental results of La2Cu(1-x)Mg(x)O4 (S=1/2) and K2Mn(1-x)Mg(x)F4 (S=5/2). The difference of xc between these materials is caused by the decrease in the magnitude of the effective spin with impurity doping. The spin gap is expected to be observed for La2Cu(1-x)Mg(x)O4 in xc
description: 6 pages, 1 figures
Hints for a Turnover at the Snow Line in the Giant Planet Occurrence Rate
Creators: name: Fernandes, Rachel B.
name: Mulders, Gijs D.
name: Pascucci, Ilaria
name: Mordasini, Christoph
name: Emsenhuber, Alexandre
Publisher: name: arXiv
Year: 2018
Subjects: subject: Solar and Stellar Astrophysics (astro-ph.SR)
subject: FOS: Physical sciences

description: The orbital distribution of giant planets is crucial for understanding how terrestrial planets form and predicting yields of exoplanet surveys. Here, we derive giant planets occurrence rates as a function of orbital period by taking into account the detection efficiency of the Kepler and radial velocity (RV) surveys. The giant planet occurrence rates for Kepler and RV show the same rising trend with increasing distance from the star. We identify a break in the RV giant planet distribution between ~2-3 au -- close to the location of the snow line in the Solar System -- after which the occurrence rate decreases with distance from the star. Extrapolating a broken power-law distribution to larger semi-major axes, we find good agreement with the ~ 1% planet occurrence rates from direct imaging surveys. Assuming a symmetric power law, we also estimate that the occurrence of giant planets between 0.1-100 au is 26.6 +7.5 -5.4% for planets with masses 0.1-20MJ and decreases to 6.2 +1.5 -1.2% for planets more massive than Jupiter. This implies that only a fraction of the structures detected in disks around young stars can be attributed to giant planets. Various planet population synthesis models show good agreement with the observed distribution, and we show how a quantitative comparison between model and data can be used to constrain planet formation and migration mechanisms.
description: 16 pages, 10 figures
Mortality and comorbidities in patients with multiple sclerosis compared with a population without multiple sclerosis: An observational study using the US Department of Defense administrative claims database
Creators: name: Cohan, Stanley
name: Moore, Alan
name: Wagner, Michael
name: Bancken, Fabrice
name: Simeone, Jason
name: Geissbühler, Yvonne
name: Fraeman, Kathy
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2015

description: AbstractBackgroundData are limited for mortality and comorbidities in patients with multiple sclerosis (MS).ObjectivesCompare mortality rates and event rates for comorbidities in MS (n=15,684) and non-MS (n=78,420) cohorts from the US Department of Defense (DoD) database.MethodsComorbidities and all-cause mortality were assessed using the database. Causes of death (CoDs) were assessed through linkage with the National Death Index. Cohorts were compared using mortality (MRR) and event (ERR) rate ratios.ResultsAll-cause mortality was 2.9-fold higher in the MS versus non-MS cohort (MRR, 95% confidence interval [CI]: 2.9, 2.7–3.2). Frequent CoDs in the MS versus non-MS cohort were infectious diseases (6.2, 4.2–9.4), diseases of the nervous (5.8, 3.7–9.0), respiratory (5.0, 3.9–6.4) and circulatory (2.1, 1.7–2.7) systems and suicide (2.6, 1.3–5.2). Comorbidities including sepsis (ERR, 95% CI: 5.7, 5.1–6.3), ischemic stroke (3.8, 3.5–4.2), attempted suicide (2.4, 1.3–4.5) and ulcerative colitis (2.0, 1.7–2.3), were higher in the MS versus non-MS cohort. The rate of cancers was also higher in the MS versus the non-MS cohort, including lymphoproliferative disorders (2.2, 1.9–2.6) and melanoma (1.7, 1.4–2.0).ConclusionsRates of mortality and several comorbidities are higher in the MS versus non-MS cohort. Early recognition and management of comorbidities may reduce premature mortality and improve quality of life in patients with MS.
Topology profile for a glutamate receptor: Three transmembrane domains and a channel-lining reentrant membrane loop
Creators: name: Dingledine, Raymond
name: Bennett, Julie A
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: We investigated the transmembrane topology of the GluR3 subunit that was translated in rabbit reticulocytes supplemented with microsomal membranes. A prolactin reporter epitope was fused to GluR3 at six locations, bracketing each of the proposed transmembrane domains. The sidedness of the epitope in the microsomal membrane was then assessed by proteinase K sensitivity. The N terminus and the entire region between M3 and M4 was extracellular, and the C terminus was intracellular by this method. Four native N-linked glycosylation sites in the amino terminus and one introduced site between M3 and M4 were utilized, confirming the extracellular location of these regions. Epitopes inserted upstream and downstream of M2 were protease sensitive and thus intracellular. Our results support a topological model for glutamate receptor subunits that consists of three transmembrane domains, M1, M3, and M4, and another domain, the proposed channel-lining M2, which forms a reentrant membrane segment with both ends facing the cytoplasm.
Pseudovelia schoenigi Hecher & Bongo 2006
Creators: name: Kment, Petr
name: Kolínová, Zdislava
Publisher: name: Zenodo
Year: 2013
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hemiptera
Veliidae
Pseudovelia
Pseudovelia schoenigi

description: Pseudovelia schoenigi Hecher & Bongo, 2006
 Pseudovelia schoenigi Hecher & Bongo, 2006: 92–98, Figs 1 –9 (original description).
Two paratypes are deposited in NMPC (exchange with Natural History Museum, Vienna):
PARATYPE (J apter.): ‘ Philippinen: Masbate Isl. / 3.5 km SE Masbate, Tugbo / Tugbo River / 2.3.1998 / leg. H. Zettel (152) [p] // 1 [hw] // J [p] // Paratypus / Pseudovelia / schoenigi sp.n., des. / Hecher & Bongo 2005 [p, red label]’ (The specimen is card-mounted, the detached male genitalia glued to the same piece of card).
PARATYPE (J apter.): ‘ Philippinen: Masbate Isl. / 2 km S Baleno / stream, 4.3.1998 / leg. H. Zettel (154) [p] // J [p] // Paratypus / Pseudovelia / schoenigi sp.n., des. / Hecher & Bongo 2005 [p, red label]’. (The specimen is card-mounted, glued on its dorsum).
 Current status. Valid species: Pseudovelia (Pseudovelia) schoenigi Hecher & Bongo, 2005.
description: Published as part of Kment, Petr & Kolínová, Zdislava, 2013, Catalogue of type specimens of true bugs (Hemiptera: Heteroptera) deposited in the National Museum, Prague, Czech Republic, pp. 821-890 in Acta Entomologica Musei Nationalis Pragae 53 (2) on page 848, DOI: 10.5281/zenodo.5740731