cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
A genetically unlinked NLR network that modulates plant immunity against diverse pathogens originated from an ancestral gene cluster
Creators: name: Wu, Chih-Hang
name: Kamoun, Sophien
Publisher: name: Zenodo
Year: 2019
Subjects: plant-microbe interactions
pathogens
immunology
plant pathology
evolution

description: NLR immune receptors in the NRC superclade form an intricate network that confers resistance to a multitude of plant pathogens. This network is comprised of sensor NLRs, specialized in detection of pathogen effectors, that require helper NLRs, known as NRCs, to initiate immune responses. The network originated from a linked NLR pair that evolved prior to the divergence of asterids and caryophyllales over 100 million years ago, and expanded to half of all NLRs in some asterid species. We used phylogenomics methods to reconstruct the evolutionary history of the NRC family. One of the NRCs, we termed NRC0, is the only family member shared among different asterid species, leading us to investigate its evolutionary history and genetic organization. In tomato, carrot, coffee and morning glory, NRC0 is closely linked to NLRs that are phylogenetically related to NRC-dependent sensors. This prompted us to hypothesize that the ancestral state of the NRC network is an NLR helper-sensor gene cluster that was present in early asterids. We validated this hypothesis by demonstrating that tomato NRC0 is essential for the hypersensitive cell death induced by its genetically linked NLR. We are testing the degree to which the functional dependency between NRC0 and the linked NLRs is conserved across asterids. These results revealed the evolutionary dynamics of NLR receptor networks and how coevolution with pathogens drivesthe expansion and diversification of NLRs.
Supplementary document for Photonic crystal backbone for light trapping inside an ultrathin, low absorbing layer - 5928926.pdf
Creators: name: El-Jallal, Said
name: hochedel, Marion
name: Capitolis, Jerôme
name: Nguyen, Hai-Son
name: CHEVALIER, CELINE
name: LECLERCQ, Jean-Louis
name: Amara, Mohamed
name: Seassal, Christian
name: Drouard, Emmanuel
Publisher: name: Optica Publishing Group
Year: 2022
Subjects: Uncategorized

description: Supplemental information
A HISTORICAL OVERVIEW OF THE DEVELOPMENT OF MEDIA AND COMMUNICATION
Creators: name: Rushana Murodullaevna Aliakbarova
Publisher: name: Zenodo
Year: 2024

description: Abstract: The historical development of media and communication is a complex and multifaceted process reflecting changes in socio-economic, political and technological conditions. This development began with ancient forms of information transmission, such as oral storytelling and handwritten documents. The invention of the printing press by Johannes Gutenberg in the mid-15th century was a revolutionary step that ushered in the era of mass production of books and newspapers, greatly expanding access to information. In the 19th century, the telegraph and telephone made a qualitative leap in the speed and scope of communication, allowing messages to be transmitted over long distances in a matter of minutes.

 

Keywords: Aristotle, Media, communication, society, newspapers, content, feedback, information, interactivity.
Geomydoecus (Geomydoecus) cliftoni Price & Hellenthal 1981
Creators: name: Sánchez-Montes, Sokani
name: Colunga-Salas, Pablo
name: Álvarez-Castillo, Lucía
name: Guzmán-Cornejo, Carmen
name: Montiel-Parra, Griselda
Publisher: name: Zenodo
Year: 2018
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Psocodea
Trichodectidae
Geomydoecus
Geomydoecus cliftoni

description: Geomydoecus (Geomydoecus) cliftoni Price & Hellenthal, 1981
Geomydoecus cliftoni Price & Hellenthal, 1981b: 40, figs 8–11.
Jalisco: Thomomys umbrinus subspecies, Tuxpán, 14 May 1967, P.L. Clifton; 5 mi (8.0 km) W Bolanos, no date; 9 mi (14.5 km) W Yahualica, no date (Price & Hellenthal 1981b).
Types: Holotype female deposited in KSUC (112246).
description: Published as part of Sánchez-Montes, Sokani, Colunga-Salas, Pablo, Álvarez-Castillo, Lucía, Guzmán-Cornejo, Carmen & Montiel-Parra, Griselda, 2018, Chewing lice (Insecta: Phthiraptera) associated with vertebrates in Mexico, pp. 1-109 in Zootaxa 4372 (1) on page 52, DOI: 10.11646/zootaxa.4372.1.1, http://zenodo.org/record/3098143
A widely conserved N-terminal motif in the coiled-coil domain of NLR immune receptors is required for activation of hypersensitive cell death
Creators: name: Adachi, Hiroaki
name: Contreras, Mauricio
name: Harant, Adeline
name: Wu, Chih-Hang
name: Derevnina, Lida
name: Sakai, Toshiyuki
name: Duggan, Cian
name: Moratto, Eleonora
name: Bozkurt, Tolga O
name: Maqbool, Abbas
name: Win, Joe
name: Kamoun, Sophien
Publisher: name: Zenodo
Year: 2019
Subjects: plant-microbe interactions
pathogens
immunity
receptors
genomics
evolution

description: The majority of plant NLR immune receptors carry an N-terminal coiled-coil domain, which mediates self-association and is required or, in some cases, sufficient for activation of hypersensitive cell death. However, our understanding of how CC-NLRs trigger cell death remains limited. Here, we used the in vitroMu transposition system to generate a random truncation library and identify the minimal region required for CC-NLR-mediated cell death. We applied this method to NRC4—a helper NLR that functions with a multitude of sensor NLRs within a complex receptor network. This revealed that the N-terminal 29 amino acids of NRC4 are sufficient to induce cell death. Remarkably, this region is defined by a motif that follows the consensus MADAxVSFxVxKLxxLL, and is conserved not only in NRC proteins but also in ~20% of all CC-NLRs of dicot and monocot species. Motif swapping experiments revealed that this sequence is functionally conserved between NLRs from distantly related plant species. Interestingly, NRC-dependent NLRs and many other sensor NLRs lack the N-terminal motif which may have become non-functional over evolutionary time. We conclude that the evolutionarily constrained “MADA motif” is critical for the cell death inducing activity of CC domains from a significant fraction of plant NLR proteins.
Synthesis, biological evaluation and molecular modeling studies of modulated benzyloxychalcones as potential acetylcholinesterase inhibitors
Creators: name: Ali, Arman Abdalla
name: Mhamad, Shakhawan Ahmad
name: Hasan, Aso Hameed
name: Ahmad, Iqrar
name: Abdullah, Siti Awanis
name: Jamil, Shajarahtunnur
name: Patel, Harun
name: Murugesan, Sankaranarayanan
name: Jamalis, Joazaizulfazli
Publisher: name: Taylor & Francis
Year: 2023
Subjects: Biophysics
Biochemistry
Neuroscience
Pharmacology
Biotechnology
Chemical Sciences not elsewhere classified
Biological Sciences not elsewhere classified

description: Acetylcholinesterase inhibitors (AChEIs) have become a significant target in the search for an efficient treatment of Alzheimer’s disease. Chalcone-based compounds display a strong potency to hinder AChE. So, this study focused on the synthesis of a series of new chalcone derivatives with anti-cholinesterase potential and their structures were characterized based on spectroscopic methods including IR, 1H NMR, 13C NMR and HRMS. Chalcone derivatives were screened against AChE. Most of them exhibited potent inhibitory activity against AChE. Compound 11i showed the most potent activity toward acetylcholinesterase compared to the positive compound, Galantamine. Docking studies into the active site of the acetylcholinesterase enzyme ravealed the significant docking score of the synthesized compounds with docking score of −7.959 to −9.277 kcal/mol when compared to the co-crystallized ligand, Donepezil (−10.567 kcal/mol). The interaction’s stability was further assessed using a conventional atomistic 100 ns dynamics simulation study, which revealed the conformational stability of representative compound 11i in the cavity of the acetylcholinesterase enzyme. Communicated by Ramaswamy H. Sarma
Geomydoecus (Geomydoecus) copei Werneck 1945
Creators: name: Sánchez-Montes, Sokani
name: Colunga-Salas, Pablo
name: Álvarez-Castillo, Lucía
name: Guzmán-Cornejo, Carmen
name: Montiel-Parra, Griselda
Publisher: name: Zenodo
Year: 2018
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Psocodea
Trichodectidae
Geomydoecus
Geomydoecus copei

description: Geomydoecus (Geomydoecus) copei Werneck, 1945
Geomydoecus copei Werneck, 1945: 114, figs 38, 39.
Puebla: Orthogeomys hispidus hispidus, 1 km NW of Zihuateutla, no date (Price & Hellenthal 1976). Querétaro: Orthogeomys hispidus concavus, Pinal de Amoles, no date (Price & Hellenthal 1976). San Luis Potosí: Orthogeomys hispidus concavus, 2 mi. (3.2 km) SW of Xilitla, no date; 37 mi. (59.6 km) S of Valles, no date (Price & Hellenthal 1976).
Tamaulipas: Orthogeomys hispidus negatus, 68.5 km S of Ciudad Victoria, no date; 70 km S of Ciudad Victoria, no date; 2 km W of El Carrizo, no date; Rancho del Cielo, no date (Price & Hellenthal 1976). Veracruz: “ Geomys mexicanus ” [in error], Misantla (Werneck 1945); Orthogeomys hispidus hispidus, 4 km of Tlapacoyan, no date; 5 mi (8.1 km) N of Jalapa, no date (Price & Hellenthal 1976); Orthogeomys hispidus torridus, Misantla, no date (Price & Emerson 1971); 12.5 mi. (19.7 km) N of Tihuatlan, no date; 3 km W of Gutiérrez Zamora, no date (Price & Hellenthal 1976).
Types: Holotype and allotype deposited in the NHML.
Notes: Werneck (1945) identified the original host as“ Geomys mexicanus ”, but Emerson & Price (1971) concluded that the correct species was Orthogeomys hispidus torridus, because this is the only pocket gopher found in Misantla.
description: Published as part of Sánchez-Montes, Sokani, Colunga-Salas, Pablo, Álvarez-Castillo, Lucía, Guzmán-Cornejo, Carmen & Montiel-Parra, Griselda, 2018, Chewing lice (Insecta: Phthiraptera) associated with vertebrates in Mexico, pp. 1-109 in Zootaxa 4372 (1) on page 52, DOI: 10.11646/zootaxa.4372.1.1, http://zenodo.org/record/3098143
Challenges in imaging and predictive modeling of rhizosphere processes
Creators: name: Roose, T.
name: Keyes, S.D.
name: Daly, K.R.
name: Carminati, A.
name: Otten, W.
name: Vetterlein, D.
name: Peth, S.
Publisher: name: Cham : Springer Nature Switzerland AG
Year: 2016
Subjects: subject: Chemical mapping
subject: Correlative imaging
subject: Mathematical modeling
subject: Rhizosphere
subject: X-ray CT
subject: Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
subject: Dewey Decimal Classification::500 | Naturwissenschaften::580 | Pflanzen (Botanik)

description: Background: Plant-soil interaction is central to human food production and ecosystem function. Thus, it is essential to not only understand, but also to develop predictive mathematical models which can be used to assess how climate and soil management practices will affect these interactions. Scope: In this paper we review the current developments in structural and chemical imaging of rhizosphere processes within the context of multiscale mathematical image based modeling. We outline areas that need more research and areas which would benefit from more detailed understanding. Conclusions: We conclude that the combination of structural and chemical imaging with modeling is an incredibly powerful tool which is fundamental for understanding how plant roots interact with soil. We emphasize the need for more researchers to be attracted to this area that is so fertile for future discoveries. Finally, model building must go hand in hand with experiments. In particular, there is a real need to integrate rhizosphere structural and chemical imaging with modeling for better understanding of the rhizosphere processes leading to models which explicitly account for pore scale processes.
SOCIO-PSYCHOLOGICAL FACTORS OF THE INDIVIDUAL SITUATION IN THE STUDENT GROUP
Creators: name: Haydarova, Sarvinoz
Publisher: name: Zenodo
Year: 2024

description: The article examines the socio-psychological factors that affect the state of a person in a group of students. Empirical research data on the establishment of relationships between different levels of the social-psychological condition of students in the group and the predominant types of relationships between students are presented.
Geomydoecus (Geomydoecus) coronadoi subsp. coronadoi Barrera 1961
Creators: name: Sánchez-Montes, Sokani
name: Colunga-Salas, Pablo
name: Álvarez-Castillo, Lucía
name: Guzmán-Cornejo, Carmen
name: Montiel-Parra, Griselda
Publisher: name: Zenodo
Year: 2018
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Psocodea
Trichodectidae
Geomydoecus
Geomydoecus coronadoi
Geomydoecus (geomydoecus) coronadoi coronadoi barrera, 1961

description: Geomydoecus (Geomydoecus) coronadoi coronadoi Barrera, 1961
Geomydoecus geomydis coronadoi Barrera, 1961: 116, figs 1, 2, 4–6.
Hidalgo: Cratogeomys perotensis, Irolo, 2500 m, no date (Light & Hafner 2007).
Tlaxcala: Cratogeomys perotensis, 8 km N Tlaxco, 2887 m, no date (Light & Hafner 2007). Puebla: Cratogeomys perotensis, 2.5 km N Zaragosa, 2250 m, no date (Light & Hafner 2007). Veracruz: Cratogeomys merriami estor, Las Vigas (Barrera 1961); 2 km and 3 km E Las Vigas, no date; 6 km SSE Altotonga, no date; 7 km SE Jalacingo, no date; Cratogeomys perotensis, 10 km SE Perote, no date; 1 mi (1.6 km) NW Pescados, no date (Price & Hellenthal 1989a); 9 km NE Perote, 2440 m, no date (Light & Hafner 2007). Note: The original host was given as “ Cratogeomys perotensis estor Merriam ” by Barrera (1961). Types: Holotype and allotype deposited in CNIABM.
description: Published as part of Sánchez-Montes, Sokani, Colunga-Salas, Pablo, Álvarez-Castillo, Lucía, Guzmán-Cornejo, Carmen & Montiel-Parra, Griselda, 2018, Chewing lice (Insecta: Phthiraptera) associated with vertebrates in Mexico, pp. 1-109 in Zootaxa 4372 (1) on page 52, DOI: 10.11646/zootaxa.4372.1.1, http://zenodo.org/record/3098143
Modeling of coupled water and heat transfer in freezing and thawing soils, Inner Mongolia
Creators: name: Zhao, Ying
name: Si, Bingcheng
name: He, Hailong
name: Xu, Jinghui
name: Peth, Stephan
name: Horn, Rainer
Publisher: name: Basel : MDPI
Year: 2016
Subjects: subject: Frozen soil module
subject: Grazing
subject: Inner Mongolia grassland
subject: Water phase change
subject: Dewey Decimal Classification::600 | Technik::690 | Hausbau, Bauhandwerk

description: Accurate simulation of soil water and heat transfer is critical to understand surface hydrology under cold conditions. Using an extended freezing code in HYDRUS-1D (freezing module), this study was conducted: (1) to evaluate the freezing module using field data collected in a grazed steppe of Inner Mongolia; and (2) to further simulate grazing effects on frozen soil hydrological processes. The experimental data consisted of soil water and temperature profiles measured during freeze-thaw cycles from 2005 to 2006 in two plots (ungrazed since 1979 (UG79) and winter grazing (WG)). To check the sensitivity of the freezing module, a model without a freezing scheme (normal module) was used for comparison. We found that while the normal module can only simulate soil water and heat transfer under unfrozen conditions, the freezing module can simulate well under both frozen and unfrozen conditions. The freezing module can reasonably compute water phase change and, therefore, substantially improved the simulation of the evolution of liquid water and temperature in frozen soil. It overestimated liquid water content during spring snowmelt and, thus, underestimated surface runoff from underlying frozen soil layers. Furthermore, the weak prediction of soil moisture at the WG site, compared with the UG79 site, might relate to the less than ideal parameterization of soil hydraulic properties. Our results confirmed that the freezing module was able to accurately predict behaviors of soil freezing and thawing, as well as the effects of land management. We suggest that detailed knowledge of the soil-atmosphere processes is needed to improve the surface runoff algorithm in the frozen soil module.
Comment on ‘Kidron, G. J. (2018). Biocrust research: A critical view on eight common hydrological-related paradigms and dubious theses. Ecohydrology, e2061’
Creators: name: Felde, Vincent John Martin Noah Linus
name: Rodriguez‐Caballero, Emilio
name: Chamizo, Sonia
name: Rossi, Federico
name: Uteau, Daniel
name: Peth, Stephan
name: Keck, Hannes
name: De Philippis, Roberto
name: Belnap, Jayne
name: Eldridge, David J.
Publisher: name: Chichester : Wiley
Year: 2020
Subjects: subject: Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
subject: Dewey Decimal Classification::500 | Naturwissenschaften::550 | Geowissenschaften

description: [No abstract available]
title: Research on the urban residential space fabric based on complex network analysis. Evidence from built-up urban area of Shanghai
Creators: name: Wang, Jiayin
name: Yang, Fan
Publisher: name: Conference Proceedings XXIX International Seminar on Urban Form ISUF 2022 Urban Redevelopment and Revitalisation A Multidisciplinary Perspective/Lodz University of Technology Press
Year: 2023
Subjects: Fesidential space fabric, Spatial evolution, Network analysis, Built environment, Shanghai.

description: The expansion of urban scale not only means the huge scale of residential population and built environment space, but also means the complexity of social network, which poses great challenges to urban governance. This research chooses the central built area of Shanghai as the research area, and focus on the 4454 residential quarters. Firstly, the paper classifies the residential area according to the residential type by identifying the residential quarters of each year based on satellite image data. Secondly, it reveals the distribution and evolution characteristics of residential quarters based on the year by year quantitative analysis of scale, type etc. Thirdly, the complex network scientific analysis method was employed to analyze the characteristics of residential space fabric from the aspects of degree distribution, network centrality and network community, to explore the characteristics and significance of the residential quarters as the network central node. It points that the community and centrality of the residential network can reflect the historical evolution of Shanghai housing. The conclusions of the research have important theoretical significance for the re-understanding of residential space fabric, the site selection planning and design at meso scale level in metropolis.
Macrotrichurus Silverstri 1946
Creators: name: Silva, Ruan Felipe Da
name: Caron, Edilson
name: Moreno, Carlos
Publisher: name: Zenodo
Year: 2025
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Coleoptera
Staphylinidae
Macrotrichurus

description: Key to species of Macrotrichurus Silverstri, 1946
1. One pair of setae on vertex of the head, ligula almost indistinct, broadly rounded, abdominal tergite VIII longer than wide, with two rows each with four bristles on surface.................................. Macrotrichurus granzottorum sp. nov.
- Two pairs of setae on vertex of the head, ligula stout and prominent, abdominal tergite VIII subquadrate, with one row of six bristles on surface.................................................................................... 2
2. Head and tergite surface without punctation, pronotum and elytra with sparse bristles, overall body covered with uniformly distributed, sparse bristles................................... M. brasiliensis Silverstri, 1946 (fig 7 in Silvestri 1946)
- Head and tergite surface with punctation, pronotum and elytra densely covered with long bristles, overall body with dense pilosity.................................................... M. notabilis Silvestri, 1946 (fig 8 in Silvestri 1946)
description: Published as part of Silva, Ruan Felipe Da, Caron, Edilson & Moreno, Carlos, 2025, Macrotrichurus granzottorum, a new species of termitophilous rove beetle from Brazil (Staphylinidae: Aleocharinae: Termitonannini), pp. 85-100 in Zootaxa 5590 (1) on page 100, DOI: 10.11646/zootaxa.5590.1.4, http://zenodo.org/record/14955875
Understanding image-text relations and news values for multimodal news analysis
Creators: name: Cheema, Gullal S.
name: Hakimov, Sherzod
name: Müller-Budack, Eric
name: Otto, Christian
name: Bateman, John A.
name: Ewerth, Ralph
Publisher: name: Lausanne : Frontiers Media
Year: 2023
Subjects: subject: computational analytics
subject: image-text relations
subject: journalism
subject: machine learning
subject: multimodality
subject: news analysis
subject: news values
subject: semiotics
subject: Dewey Decimal Classification::000 | Allgemeines, Wissenschaft::000 | Informatik, Wissen, Systeme::004 | Informatik

description: The analysis of news dissemination is of utmost importance since the credibility of information and the identification of disinformation and misinformation affect society as a whole. Given the large amounts of news data published daily on the Web, the empirical analysis of news with regard to research questions and the detection of problematic news content on the Web require computational methods that work at scale. Today's online news are typically disseminated in a multimodal form, including various presentation modalities such as text, image, audio, and video. Recent developments in multimodal machine learning now make it possible to capture basic “descriptive” relations between modalities–such as correspondences between words and phrases, on the one hand, and corresponding visual depictions of the verbally expressed information on the other. Although such advances have enabled tremendous progress in tasks like image captioning, text-to-image generation and visual question answering, in domains such as news dissemination, there is a need to go further. In this paper, we introduce a novel framework for the computational analysis of multimodal news. We motivate a set of more complex image-text relations as well as multimodal news values based on real examples of news reports and consider their realization by computational approaches. To this end, we provide (a) an overview of existing literature from semiotics where detailed proposals have been made for taxonomies covering diverse image-text relations generalisable to any domain; (b) an overview of computational work that derives models of image-text relations from data; and (c) an overview of a particular class of news-centric attributes developed in journalism studies called news values. The result is a novel framework for multimodal news analysis that closes existing gaps in previous work while maintaining and combining the strengths of those accounts. We assess and discuss the elements of the framework with real-world examples and use cases, setting out research directions at the intersection of multimodal learning, multimodal analytics and computational social sciences that can benefit from our approach.
Calandrella minor subsp. aharonii Hartert
Creators: name: LeCROY, M. A. R. Y.
Publisher: name: Zenodo
Year: 2003
Subjects: Biodiversity
Taxonomy
Animalia
Chordata
Aves
Passeriformes
Alaudidae
Calandrella
Calandrella minor
Calandrella minor aharonii hartert

description: Calandrella minor aharonii Hartert
 Calandrella minor aharonii Hartert, 1910e: 13 (Karyatein).
 Now Calandrella rufescens aharonii Hartert, 1910. See Cramp, 1988: 135.
HOLOTYPE: AMNH 556138, adult male, collected at El Qaryatein (= Karyatein, Vaurie, 1959: 32), 34°13′N, 37°13′E (Times Atlas), Syria, on 25 March 1910, by J. Aharoni. From the Rothschild Collection.
COMMENTS: Although Hartert (1910e: 13) mentioned that Aharoni collected large numbers of this form at El Qaryatein, he (1919: 164) said: ‘‘So far all I have seen of this interesting Lark are six specimens collected in March and February at Karyatein.’’ Only three paratypes came to AMNH with the Rothschild Collection: AMNH 556139– 556141. The holotype is the only specimen in AMNH collected by Aharoni on 25 March 1910, the date given in the original description.
description: Published as part of LeCROY, M. A. R. Y., 2003, TYPE SPECIMENS OF BIRDS IN THE AMERICAN MUSEUM OF NATURAL HISTORY. PART 5. PASSERIFORMES: ALAUDIDAE, HIRUNDINIDAE, MOTACILLIDAE, CAMPEPHAGIDAE, PYCNONOTIDAE, IRENIDAE, LANIIDAE, VANGIDAE, BOMBYCILLIDAE, DULIDAE, CINCLIDAE, TROGLODYTIDAE, AND MIMIDAE, pp. 1-156 in Bulletin of the American Museum of Natural History 278 (278) on page 17, DOI: 10.1206/0003-0090(2003)278<0001:tsobit>2.0.co;2, http://zenodo.org/record/13256611
The Open Science MOOC for the UNESCO Capacity Building Working Group
Creators: name: Masuzzo, Paola Chiara
Publisher: name: Zenodo
Year: 2022
Subjects: Open Science
MOOC
UNESCO

description: The UNESCO Open Science Working Group on Capacity Building met for the first time on the 12th of May 2022. I had the honour to give a short presentation about the Open Science MOOC, its goal, community, and current development missions.
Molecular and serological evidences of Midichloria mitochondrii transmission to vertebrate hosts during the tick bite
Creators: name: Serra, Valentina
name: Cafiso, Alessandra
name: Bazzocchi, Chiara
Publisher: name: Department of Health, Animal Science and Food Safety (VESPA)
Year: 2016
Subjects: Ixodes ricinus; Midichloria mitochondrii; Serological screening; Mammalian hosts; Marker

description: The tick Ixodes ricinus is vector of many pathogens important for human and animal health (Parola and Raoult, 2001; Socolovschi, 2009). Midichloria mitochondrii (order Rickettsiales; family Midichloriaceae) is an endosymbiont present in the salivary glands of 100% of Ixodes ricinus females. Two lines of evidence suggest a transmission of M. mitochondrii in mammalians during the tick bite: 1) detection of circulating DNA in blood samples of different animal species; 2) seropositivity toward a M. mitochondrii protein (FliD) in humans and dogs exposed to tick bite (Mariconti 2012; Bazzocchi 2013). Here we present serological and molecular results demonstrating the circulation of M. mitochondrii also in Capreolus capreolus (the host of choice for adult and nymph stages of I. ricinus), confirming that this host is a good subject to study the spread of tick-borne pathogens.

Based on these results, FliD protein and other M. mitochondrii markers could thus be extremely useful to determine the risk of infection by I. ricinus pathogens in given areas, and for investigating the epidemiological association of a variety of pathological alterations with this tick.

Here we show results of the screening of 218 human sera (50 from non endemic areas used as negative controls and 168 from subjects exposed to tick bite) collected in different areas of Germany. Results showed that 48 out of 168 sera were positive to M. mitochondrii.

These results have posed the basis for the development of a serological test for investigating the exposure of humans and animals to this tick species.
description: International Journal of Health, Animal Science and Food Safety, Vol 3, No 1s (2016)
Geomydoecus (Geomydoecus) coronadoi subsp. saccharalis Price & Hellenthal 1989
Creators: name: Sánchez-Montes, Sokani
name: Colunga-Salas, Pablo
name: Álvarez-Castillo, Lucía
name: Guzmán-Cornejo, Carmen
name: Montiel-Parra, Griselda
Publisher: name: Zenodo
Year: 2018
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Psocodea
Trichodectidae
Geomydoecus
Geomydoecus coronadoi
Geomydoecus (geomydoecus) coronadoi saccharalis price & hellenthal, 1989

description: Geomydoecus (Geomydoecus) coronadoi saccharalis Price & Hellenthal, 1989
Geomydoecus coronadoi saccharalis Price & Hellenthal, 1989a: 394. Puebla: Cratogeomys merriami saccharalis, 2 mi (3.2 km) S Atlixco, 9 Aug. 1954, R.W. Dickerman (Price & Hellenthal, 1989a).
Types: Holotype female deposited in KSUC (62518).
description: Published as part of Sánchez-Montes, Sokani, Colunga-Salas, Pablo, Álvarez-Castillo, Lucía, Guzmán-Cornejo, Carmen & Montiel-Parra, Griselda, 2018, Chewing lice (Insecta: Phthiraptera) associated with vertebrates in Mexico, pp. 1-109 in Zootaxa 4372 (1) on page 52, DOI: 10.11646/zootaxa.4372.1.1, http://zenodo.org/record/3098143
Supplementary document for Raman-induced frequency shift of pure quartic solitons in optical fiber with quartic dispersion - 5928959.pdf
Creators: name: Wang, Zhi
name: chang-you, luo
name: Wang, Youwen
name: Ling, Xiaohui
name: ZHANG, LIFU
Publisher: name: Optica Publishing Group
Year: 2025
Subjects: Uncategorized

description: RIFS_of_PQS_with_loss
Widespread extrahepatic expression of acute-phase proteins in chicken (Gallus gallus) tissues
Creators: name: Marques, Andreia Tomás
name: Lecchi, Cristina
name: Nordio, Laura
name: Giudice, Chiara
name: Grilli, Guido
name: Ariño-Bassols, Helena
name: Ceciliani, Fabrizio
Publisher: name: Department of Health, Animal Science and Food Safety (VESPA)
Year: 2016
Subjects: Acute phase proteins, Animal welfare, Chicken, Gallus gallus

description: Acute Phase Proteins (APP) are plasma proteins that can modify their expression in response to inflammation caused by tissue injury, infections, immunological disorders, or stress. Although APP are produced mainly in liver, extrahepatic production has been described (Marques et al., 2016; Lecchi et al., 2012). The aim of this work was to study the extrahepatic gene expression of five APP, namely α1-acid glycoprotein (AGP), Serum amyloid A (SAA), PIT54, C-rective protein (CRP) and Ovotransferrin (OVT) (O'Reilly and Eckersall, 2014) in different healthy chicken (Gallus gallus) tissues by quantitative real time PCR (qPCR) and immunohistochemistry to detect the precise location of the proteins.

APP gene expression was higher in liver compared with other tissues. mRNA coding for CRP, OVT and SAA was detected in all tissues involved in this study with a higher expression in gastrointestinal tract, respiratory system and lymphatic system. SAA expression was particularly high in cecal tonsil, lung, spleen and meckel’s diverticulum, whereas OVT showed a high expression in lung, bursa of Fabricius, pancreas, brain and adipose tissue. AGP and PIT54 was also detected in pericardial adipose tissue, spleen, kidney, lung, mucosa of proventriculus, mucosa of gizzard and pancreas but, oppositely to SAA, their mRNA was not detected in meckel’s diverticulum, cecal tonsil and bursa of Fabricius. These results suggest that each tissue is able to express different amount of APP even in healthy conditions and mount a local acute phase reaction. Immunohistochemistry to detect the precise location for AGP, OVT and SAA using available antibodies is ongoing.
description: International Journal of Health, Animal Science and Food Safety, Vol 3, No 1s (2016)
Evaluation of antibiotic and cell-based therapy in preventing S. epidermidis-induced nonunion in rats
Creators: name: Bottagisio, Marta
name: Drago, Lorenzo
name: Romanò, Carlo Luca
name: Bonizzi, Luigi
name: Lovati, Arianna Barbara
Publisher: name: Department of Health, Animal Science and Food Safety (VESPA)
Year: 2016
Subjects: Methicillin-resistant S. epidermidis; Nonunion; Mesenchymal stem cells; Rat model; Osteomyelitis

description: Methicillin-resistant S. epidermidis (MRSE) is responsible for biofilm-related infections (Montanaro, 2011; Romanò, 2013) and fracture nonunion, as recently demonstrated by our group (Lovati, 2016).The present study aims to investigate the efficacy of antibiotic or cell-based therapies in preventing bacterial infections and nonunion establishment.Under anesthesia, femoral fractures were performed in 30 rats, then the site of injury was injected with a clinical-derived MRSE strain and, finally, synthesized with stainless steel plates. Rats were differently treated as follows: MRSE-infected controls (IC); systemically-injected vancomycin (s-VANC); local vancomycin-enriched hydrogel (l-HYD); systemically-injected BMSCs (s-BMSCs); and locally-injected BMSCs (l-BMSCs).After 6 weeks, pro-inflammatory cytokines, quantitative micro-CT, histological and microbiological analyses were carried out to investigate the host response to the different treatments.Half of the s-BMSCs rats died closely to the systemic cell injection, thus excluded for further analyses. Our results for the IC group were consistent with previously published data (Lovati, 2016), showing signs of osteomyelitis and nonunion development. In s-VANC and l-HYD groups, micro-CT detected a good bony bridging and the microbiological counts were significantly lower with respect to the other groups. Our study suggests that the association of s-VANC and l-HYD is an effective treatment to prevent biofilm-induced nonunions. Differently, we cannot positively support cell therapies for this purpose due to the high risk related to the systemic cell injection, thus requiring further studies to be eventually proposed in clinics.
description: International Journal of Health, Animal Science and Food Safety, Vol 3, No 1s (2016)
Study of milling time impact on hydrogen desorption from LiAlH4-Fe2O3 composites
Creators: name: Milanovic, Igor
name: Milosevic-Govedarovic, Sanja
name: Lukic, Miodrag
name: Jovanovic, Zoran
name: Rmus, Jelena
name: Mitrovic-Rajic, Andjela
name: Grbovic-Novakovic, Jasmina
name: Kurko, Sandra
Publisher: name: Novi Sad : Faculty of Technology, University of Novi Sad
Year: 2022
Subjects: subject: composites
subject: hydrides
subject: hydrogen storage
subject: kinetics
subject: mechanochemical synthesis
subject: Dewey Decimal Classification::600 | Technik::620 | Ingenieurwissenschaften und Maschinenbau
subject: Dewey Decimal Classification::600 | Technik::670 | Industrielle und handwerkliche Fertigung

description: LiAlH4 was modified by mechanical milling and with the addition of 5 wt.% Fe2O3 in order to improve its hydrogen desorption properties. The composite was milled for 1, 3, 5, 7 or 15 min, and depending on the milling time, various phenomena took place. Up to a milling time of 5 min, the particle size of the composite decreases. Further milling leads to the particles agglomeration reaching the size of the starting material after 15 min. Moreover, the mechanical milling process leads to the transformation of AlH4– to AlH63 – structure as a result of partial hydrogen desorption. Hydrogen desorption during the milling is the most pronounced in the sample milled for 15 min, so this sample has only one hydrogen desorption peak in the temperature-programmed desorption measurements. Mechanical milling with the addition of Fe2O3 for up to 15 min improves LiAlH4 hydrogen desorption properties as hydrogen desorption temperature and apparent activation energies decrease.
Role of the mononuclear phagocyte system in the uptake and accumulation of nanoparticles designed for biomedical applications
Creators: name: De Maglie, Marcella
Publisher: name: Department of Health, Animal Science and Food Safety (VESPA)
Year: 2016
Subjects: Nanoparticles, Nanomedicine, Mononuclear Phagocyte System

description: The unique physical-chemical properties of Nanoparticles (NPs) make them suitable for many biomedical applications (drug delivery, imaging, cell tacking) (Yildirimer et al, 2011). However the design of nanoparticle-based platforms for nanomedicine is extremely challenging and must overcome a number of physiologically constraints, including the Mononuclear Phagocyte System (MPS) (Dawidczyk et al, 2014).
 The aim of this study was to assess the role of MPS in the biodistribution and accumulation of metallic NPs [iron-oxide NPS and silver NPs (AgNPs)] after single intravenous administration in mice. 24 hours after the treatment mice were sacrificed and underwent complete necropsy. Liver, kidney, spleen and lung were collected, stained with hematoxylin and eosin (HE), Perls stain (for iron-oxide NPs) or autometallography (for AgNPs), and evaluated under a light microscope. Immunostaining for Iba-1 (pan macrophage marker) was applied to localize the NPs aggregates within MPS cells.
 Histologically, in all treated mice, intracytoplasmic brown granular material (consistent with iron or silver pigment, as confirmed by Perls iron stain and autometallography) was found in stained sections of the liver (within Kupffer cells), spleen (MPS of marginal zone and red pulp) and lung (MPS within alveolar septa). Iron/silver deposits were mainly found in the cytoplasm of MPS cells (Iba1 immunostaining), indicating that most of injected particles were removed from blood circulation by phagocytic cells. These findings indicate that MPS greatly influence NPs biodistribution. For this reason, strategies able to increase bioavailability of NPs by minimizing MPS-mediated clearance are needed. Moreover the potential toxic effects due to the persistence of NPs in MPS should be further investigated.
description: International Journal of Health, Animal Science and Food Safety, Vol 3, No 1s (2016)
Zwischen virtuoser Unverbindlichkeit und Herrschaft: Lebensentwürfe von Vätern als Leerstelle in der Kindesschutzarbeit
Creators: name: Brauchli, Simone
Publisher: name: (:null)
Year: 2024
Subjects: H Social Sciences (General)
HQ The family. Marriage. Woman

description: Kindesschutzarbeit konzentriert sich, so zeigt es die Empirie, weltweit vorrangig auf Mütter, während Väter oft vernachlässigt werden. Doch inwieweit sind Väter Teil von  „Kindesschutzfamilien“ und ihren Problemen? Und wie werden sie von Fachkräften adressiert und in Unterstützungsmassnahmen einbezogen? Im vorliegenden Beitrag werden diese Fragen anhand von empirischem Material aus einer abgeschlossenen ethnographischen Untersuchung zur Sozialpädagogischen Familienbegleitung in der Schweiz beantwortet. Die analysierten Fälle illustrieren eine doppelte Unverbindlichkeit der Väter gegenüber der Familie und dem sozialpädagogischen Angebot und verweisen auf das spannungsreiche Nebeneinander von Privilegierung und Benachteiligung von Vätern in der Sozialpädagogischen Familienbegleitung. Im Beitrag wird argumentiert, dass durch eine vorschnelle fachliche Verantwortungsentlastung der Väter familiale Konflikte reproduziert werden können und Bildungspotenziale und Autonomiemöglichkeiten ungenutzt bleiben.
description: As research has shown, child protection is primarily focused on mothers, while fathers are often not addressed. But to what extent are fathers part of ”child protection families“? And how are they addressed by social workers? This article answers these questions based on a completed ethnographic study of family support services in Switzerland. The cases illustrate a double non-commitment of fathers towards the family and social work interventions. The study points to the tense coexistence of privilege and disadvantage of fathers in family support services. The article argues that relieving fathers of their responsibilities can reproduce family conflicts and leave autonomy potential untapped.
Cortisol fetal fluids concentrations and newborn outcome in term pregnancy small-sized purebred dogs
Creators: name: Bolis, Barbara
name: Peric, Tanja
name: Rota, Alessandro
name: Faustini, Massimo
name: Veronesi, Maria Cristina
Publisher: name: Department of Health, Animal Science and Food Safety (VESPA)
Year: 2016
Subjects: , term pregnancy, fetal fluids, cortisol, newborn outcome

description: In order to provide further information about canine perinatology, and because of the scarce knowledge about fetal fluids composition in dogs, the present study was aimed to assess the cortisol concentrations in fetal fluids collected from small-sized purebred newborn puppies born by elective cesarean section, at term of pregnancy (Meloni et al, 2014), and to assess possible effects of amniotic and allantoic cortisol concentrations and newborn outcome at 24 hours of age and on newborn gender . Fetal fluids cortisol concentrations were also evaluated for correlation with maternal parity, litter-size, neonatal gender, birth weight and Apgar score (Veronesi et al, 2009). The study, performed on 50 born alive, normal weighed puppies, without gross physical malformation, showed that cortisol concentration was higher in allantoic than in amniotic fluid (p<0.01), even if a strong positive correlation between the two fluids cortisol concentration was found (p<0.0001; R=0.83). Interestingly, higher amniotic (p<0.05) cortisol concentrations were associated to puppies not surviving at 24 hours after birth. Therefore it could be suggested that this parameter may be useful for the recognition, at birth, of puppies needing special surveillance in the first day of age.  In relation to the other evaluated parameters, no correlations with amniotic or allantoic cortisol concentrations were found. In conclusion, the present results showed that in small-sized purebred puppies, born at term by elective caesarean section, the evaluation of amniotic cortisol concentration seems useful for the detection of puppies that need special surveillance during the first 24 hours of age, and should be coupled to the newborn evaluation by Apgar score.
description: International Journal of Health, Animal Science and Food Safety, Vol 3, No 1s (2016)
Geomydoecus (Geomydoecus) crovelloi Price & Hellenthal 1981
Creators: name: Sánchez-Montes, Sokani
name: Colunga-Salas, Pablo
name: Álvarez-Castillo, Lucía
name: Guzmán-Cornejo, Carmen
name: Montiel-Parra, Griselda
Publisher: name: Zenodo
Year: 2018
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Psocodea
Trichodectidae
Geomydoecus
Geomydoecus crovelloi

description: Geomydoecus (Geomydoecus) crovelloi Price & Hellenthal, 1981
Geomydoecus crovelloi Price & Hellenthal, 1981a: 18, figs 32–34.
Sonora: Thomomys umbrinus sonoriensis, 1 mi (1.6 km) N Sahuaripa 19 Jul. 1975, J.L. Patton; 3.5 mi (5.6 km) S Moctezuma, no date; E bank and W bank Río Yaqui at El Novillo, no date (Price & Hellenthal 1981a). Types: Holotype male deposited in UMSP.
description: Published as part of Sánchez-Montes, Sokani, Colunga-Salas, Pablo, Álvarez-Castillo, Lucía, Guzmán-Cornejo, Carmen & Montiel-Parra, Griselda, 2018, Chewing lice (Insecta: Phthiraptera) associated with vertebrates in Mexico, pp. 1-109 in Zootaxa 4372 (1) on page 53, DOI: 10.11646/zootaxa.4372.1.1, http://zenodo.org/record/3098143
New effectors from the multihost blast fungus Magnaporthe oryzae target HMA domain containing host proteins
Creators: name: Petit, Yohann
name: Win, Joe
name: Langner, Thorsten
name: Harant, Adeline
name: Terauchi, Ryohei
name: Banfield, Mark
name: Kamoun, Sophien
Publisher: name: Zenodo
Year: 2019
Subjects: plant-microbe interactions
fungi
effectors
pathogens
plant pathology
resource

description: Magnaporthe oryzaeis a multihost pathogen that infects >50 species of grasses, including the cereal crops wheat, barley and rice. This pathogen deploys an arsenal of secreted proteins called effectors to infect its host plants and counteract plant defences. How M. oryzae effectors have adapted to various grass hosts is poorly understood. Three effectors, AVR-Pia, AVR1-CO39 and AVR-Pik are known to bind sHMA proteins but can also be recognized by NLR resistance proteins with integrated HMA domains. The aim of this project is to discover new M. oryzaeeffectors that interact with sHMA proteins, and determine the diversification of these effectors in host-specific lineages of M. oryzae. First, we predicted candidate effectors from the secretomes of M. oryzaestrains infecting different grass hosts. We used two major criteria: i) proteins with sequence similarity to known avirulence effectors; ii) Hidden Markov Model profile matches to the MAX structural fold. This resulted in 185 potential effector candidates, which we synthesized and cloned as level 0 Golden Gate vectors, and publicly released as a community bioresource. We have been testing these candidates for their ability to interact with sHMA proteins using yeast two-hybrid screens. We have now identified and validated several new effector-HMA interacting pairs. We will build on this work to improve our understanding of the evolution and diversification of HMA-targeting effectors in M. oryzae.
ANALYTICAL ASPECTS OF PROTEIN CARBONYL GROUP IN OXIDIZED CANINE SERUM
Creators: name: Ruggerone, Beatrice
name: Colombo, Graziano
name: Paltrinieri, Saverio
Publisher: name: Department of Health, Animal Science and Food Safety (VESPA)
Year: 2016
Subjects: Dog; Western blotting; Carbonyl group proteins; Oxidative stress; Oxidants.

description: Oxidative stress (OS) is an imbalance between oxidants and anti-oxidants and plays an important role in the aetiology and/or the progression of several diseases. Protein carbonyl (PCO) groups are so far used as biomarkers of OS in humans (Colombo et al, 2015).

The aim of our study was to investigate whether PCOs are present in canine serum and if they can be measured spectrophotometrically using a method not yet validated in dogs.

The presence of PCO was investigated by Western blotting after separation by SDS-PAGE of serum at different dilutions (either before or after oxidation with 10% cigarette smoke extract). Protein labelling with 2,4-Dinitrophenylhydrazine (DNPH, Brady's reagent) was followed by a two-steps incubation with primary anti-dinitrophenyl-KLH antibodies (rabbit IgG fraction) and secondary goat anti-rabbit IgG, HRP conjugate. Signal was developed with Enhanced Chemiolumionescence (ECL).

Serum PCO were quantified using a commercially available assay (Protein Carbonyl Content Assay Kit - Abcam, UK), based on derivatization of proteins with 1,4-dinitrophenylhydrazine (DNPH) and subsequent formation of protein-conjugated dinitrophenylhydrazones (DNPs) with a peak absorbance at 366 nm.

Western blot showed an evident band of apparent MW of 69 kDa, consistent with carbonylated dog serum albumin. The spectrophotometric assay failed to demonstrate any signal at 366 nm using the manufacturer’s instructions or modified protocols.

This study demonstrated that PCO are present in oxidized canine serum. However, the spectrophotometric assay employed in this study is not enough sensitive to detect PCOs  Further studies are needed to assess whether this depends on the poor re-solubilisation of DNPs, or on the low concentration of PCOs in dogs.
description: International Journal of Health, Animal Science and Food Safety, Vol 3, No 1s (2016)
Giochi ambientali. Sensibilizzare e co-progettare azioni per l'European Green Deal all'interno di processi partecipativi
Creators: name: Fontana, Cassandra
name: Zetti, Iacopo
name: Rossi, Maddalena
name: Testi, Andrea
Publisher: name: Zenodo
Year: 2024

description: Academic Conference Presentation
A reverse transcription loop-mediated isothermal amplification (LAMP) assay for the detection of feline Coronavirus
Creators: name: Stranieri, Angelica
name: Lauzi, Stefania
name: Giordano, Alessia
name: Paltrinieri, Saverio
Publisher: name: Department of Health, Animal Science and Food Safety (VESPA)
Year: 2016
Subjects: LAMP, feline coronavirus, PCR

description: Loop-mediated isothermal amplification (LAMP) is a molecular method that amplifies DNA sequences under isothermal conditions. It relies on the use of 4 different primers recognizing 6 regions of the template sequence and on the use of a DNA polymerase with strand displacement activity. The addition of two loop primers allows the reaction time to be of one hour only. The aim of this study was to develop a reverse transcription LAMP assay for an easy and inexpensive detection of feline Coronavirus (FCoV) on different specimens. Six primers binding the conserved 3’UTR region of the FCoV were designed with the Primer Explorer software. Thirty-two samples of RNA (11 feces, 8 effusions, 9 blood samples and 4 tissues) on which a reverse transcription polymerase chain reaction (RT-PCR) for the 3’UTR region was performed were used. The reaction was carried out in 25µL reaction volume and the mixture was incubated in a thermocycler at 63°C for 1 hour followed by 10 minutes at 80°C for inactivation. LAMP products were then visualized under UV after electrophoresis migration on a 1.5% agarose gel stained with ethidium bromide, where they produce a ladder-like pattern if positive. Results where compared with those obtained on standard PCR. Sensitivity and specificity were respectively 60% and 100% on feces, 40% and 100% on effusions, 25% and 100% on blood, and 100% and 100% on tissues. The overall sensitivity and specificity of this method were of 57.1% and 100%, thus limiting a clinical application of this method, except for tissues.
description: International Journal of Health, Animal Science and Food Safety, Vol 3, No 1s (2016)