CarboCaptus
Creators: name: Inojosa, Vince Gerald
Publisher: name: Zenodo
Year: 2025

description: I just need an experts opinion on this

 

*Name: Carbocaptus

 

*Type: Microorganism Consortium

 

*Function: Captures CO2, produces oxygen, and promotes environmental sustainability

 

*Stability Rating: 100%

*Danger Level: 0%

*Chance to Fail: 0.01%

 

*Composition:

• Halo-Neapo

• Syne-Methylo

• Alphaproteobacterium

• Pseudomonas putida

• Rhizobium leguminosarum

• Pseudomonas aeruginosa

• Mycorrhizal fungi

• Bacillus subtilis

• Nitrobacter winogradskyi

• Genetic kill switch

• Self-limiting gene expression

• DNA fragmentation

• Auxotrophy

• Recombinase-based containment

 

*Description: Carbocaptus is a highly advanced, genetically engineered microorganism consortium designed to capture CO2, produce oxygen, and promote environmental sustainability.

 

*Detailed step-by-step guide to creating Carbocaptus:

 

Step 1: Design the Genome (Weeks 1-4)

1. Define the desired characteristics of Carbocaptus, including CO2 capture, oxygen production, and environmental sustainability.

2. Use computer-aided design (CAD) software to design the genome, incorporating necessary genes and regulatory elements.

3. Utilize online databases, such as GenBank or UniProt, to identify and select suitable genes for CO2 capture and oxygen production.

4. Design and optimize the genome using computational tools, such as Genome Compiler or Geneious.

 

Step 2: Choose a Host Microorganism (Weeks 5-8)

1. Research and select a suitable host microorganism, such as E. coli or Bacillus subtilis, based on factors like growth rate, genetic tractability, and environmental tolerance.

2. Obtain the host microorganism from a reputable source, such as the American Type Culture Collection (ATCC).

3. Verify the identity and purity of the host microorganism using techniques like PCR, sequencing, or microscopy.

 

Step 3: Gene Editing (Weeks 9-16)

1. Design and synthesize guide RNAs (gRNAs) and primers for CRISPR-Cas9 gene editing.

2. Prepare the host microorganism for gene editing by growing it in a suitable medium and inducing competence.

3. Perform CRISPR-Cas9 gene editing to introduce the designed genome into the host microorganism.

4. Verify the success of gene editing using techniques like PCR, sequencing, or microscopy.

 

Step 4: Synthetic Biology (Weeks 17-24)

1. Design and construct artificial biological pathways for CO2 capture and oxygen production.

2. Utilize online databases and computational tools to identify and select suitable enzymes and regulatory elements.

3. Assemble the artificial pathways using techniques like Gibson Assembly or Golden Gate Assembly.

4. Verify the functionality of the artificial pathways using techniques like enzyme assays or gas chromatography.

 

Step 5: Bioreactor Cultivation (Weeks 24-32)

1. Design and set up a bioreactor system for cultivating Carbocaptus.

2. Optimize growth conditions, such as temperature, pH, and nutrient supply, to maximize Carbocaptus growth and productivity.

3. Monitor and control the bioreactor system using sensors and automation software.

4. Harvest and process Carbocaptus biomass for further analysis and application.

 

Step 6: Testing and Validation (Weeks 32-40)

1. Conduct thorough testing and validation of Carbocaptus, including its safety, efficacy, and scalability.

2. Evaluate Carbocaptus performance using techniques like gas chromatography, spectrophotometry, or microscopy.

3. Assess Carbocaptus stability and robustness under various environmental conditions.

4. Refine and optimize Carbocaptus design and cultivation conditions based on testing and validation results.

 

Please note that this is a general outline, and actual creation of Carbocaptus may require additional steps, expertise, and resources.

 

*simplified step-by-step guide to creating Carbocaptus:

 

Step 1: Design Genome

- Use computer software to design the Carbocaptus genome, incorporating necessary genes for CO2 capture, oxygen production, and environmental sustainability.

 

Step 2: Choose Host Microorganism

- Select a suitable host microorganism, such as E. coli or Bacillus subtilis, to serve as the foundation for Carbocaptus.

 

Step 3: Gene Editing

- Utilize CRISPR-Cas9 gene editing tool to introduce the designed genome into the host microorganism.

 

Step 4: Synthetic Biology

- Construct and test artificial biological pathways for CO2 capture and oxygen production.

 

Step 5: Bioreactor Cultivation

- Cultivate Carbocaptus in a bioreactor, optimizing growth conditions and scaling up production.

 

Step 6: Testing and Validation

- Conduct thorough testing and validation of Carbocaptus, ensuring its safety, efficacy, and scalability.

 

Please note that this is a highly simplified outline, and actual creation of Carbocaptus would require extensive research, expertise, and resources.

 

*It can only survive in the sky where co2 and nitrogen are abundant

 

•Creator of CarboCaptus- Vince Gerald G. Inojosa

 

Ps: I'm not a professional or anything just thought of it and noted it, in fact I'm still 13 so you probably wouldn't trust me but you should try testing it if it works I would be very happy if it did and message me if it has problems during testing or if it is effective or not thanks for reading :)