Pseudofusicoccum ardesiacum Pavlic, T. I. Burgess & M. J. Wingf.
Creators: name: Senwanna, Chanokned
name: Hongsanan, Sinang
name: Hyde, Kevin D.
name: Cheewangkoon, Ratchadawan
name: Konta, Sirinapa
name: Wang, Yong
Publisher: name: Zenodo
Year: 2020
Subjects: Biodiversity
Taxonomy
Fungi
Ascomycota
Dothideomycetes
Botryosphaeriales
Botryosphaeriaceae
Pseudofusicoccum
Pseudofusicoccum ardesiacum

description: Pseudofusicoccum ardesiacum Pavlic, T.I.Burgess & M.J.Wingf.   (Fig. 4)  
 Mycologia 100: 858 (2008).   
INDEX FUNGORUM NUMBER. — IF512051.  
FACESOFFUNGI NUMBER. — FoF 05799.  
CULTURE CHARACTERISTICS. — Conidia germinating on MEA within 24 hours at room temperature (25-30°C) and germ tube was produced from the ends of the conidia. Initially aerial mycelium white, circular, fairly fluffy with sparse aspects, after10 days become whitish grey, velvety, raise, dense, after 20-25 days of incubation, colonies become iron grey to black, hyphae septate, branched, smooth, forming conidiomata at the colony margin after 30-40 days and after 4 months of incubation, no asexual-morph produced on culture.  
 MATERIAL EXAMINED. — Thailand. Phayao Province, Muang District, associated with canker disease on branches of Hevea brasiliensis (Euphorbiaceae), 2.X.2016, C. Senwanna, RBPY 04, MFLU 19-0235, living culture MFLUCC 17-0323.   
ADDITIONAL GENBANK NUMBER. — SSU = MK 503145.  DESCRIPTION  
 Associated with canker disease on branches of Hevea brasiliensis.    Sexual morph   
Undetermined.   Asexual morph   
 Conidiomata. (140-)175-255(-260) diam × (120-)160- 215(-220) µm high (ẍ = 201 × 189 µm, n = 10), pycnidial, solitary to scattered, immersed to semi-immersed, globose to subglobose, uni- to multiloculate, with a central ostiole.   
 Conidiomata wall. 17-46 µm wide, outer layers dark brown to black, inner layers thin-walled, hyaline, composed of cell of textura angularis.   
 Paraphyses. Absent.   
 Conidiophores. Reduced to conidiogenous cells.   
 Conidiogenous cells. (8-)9-13(-15) × (2-)2-3.5(-4) µm (ẍ = 10.4 × 2.8 µm, n = 25), holoblastic, cylindrical to sub-cylindrical, hyaline, smooth-walled.   
 Conidia. (20-)22-27(-29) × (5.5-)6-8.5(-9) µm (ẍ = 24.8 × 7.6 µm, n = 70), ellipsoid to rod-shaped,hyaline, aseptate, straight or slightly bent or irregularly shaped, smooth-walled, with fine granular content, surrounded by a thin mucilaginous sheath.   
Notes  
 Asexual morphology of our fresh collection resembles to P. ardesiacum (ex-type) in Pavlic et al. (2008) in having ellipsoid to rod-shaped, hyaline, aseptate, straight or irregularly shaped conidia. In a phylogenetic analysis of combined of ITS, LSU, TEF1 and β -tubulin gene sequence data (Figs 1; 4) our isolates clustered with P. ardesiacum. However, conidia of our strain are shorter (20-29 µm versus 17.5-32 µm) than the type (Pavlic et al. 2008). This may be due to different substrates and lifestyle of the fungi as our strain was found on Hevea brasiliensis and were observed directly from the host, while the type strain was found on Adansonia gibbosa and characters were observed in culture on pine needles (Pavlic et al. 2008). Based on morphological characters and phylogenetic analyses, we identified our collection as P. ardesiacum and report a new host record for P. ardesiacum from Hevea brasiliensis (Euphorbiaceae) in Thailand for the first time.
description: Published as part of Senwanna, Chanokned, Hongsanan, Sinang, Hyde, Kevin D., Cheewangkoon, Ratchadawan, Konta, Sirinapa & Wang, Yong, 2020, First report of the sexual morph of Pseudofusicoccum adansoniae Pavlic, T. I. Burgess & M. J. Wingf. on Para rubber, pp. 133-146 in Cryptogamie, Mycologie 20 (7) on page 142, DOI: 10.5252/cryptogamie-mycologie2020v41a7, http://zenodo.org/record/12214873