Ophiocordyceps ovatospora H. Yu & D. X. Tang 2022, sp. nov.
Creators: name: Tang, Dexiang
name: Zhu, Juye
name: Luo, Lijun
name: Hou, Donghai
name: Wang, Zhiqin
name: Yang, Shidao
name: Yu, Hong
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Fungi
Ascomycota
Sordariomycetes
Hypocreales
Ophiocordycipitaceae
Ophiocordyceps
Ophiocordyceps ovatospora

description: Ophiocordyceps ovatospora H. Yu & D.X. Tang, sp. nov. Fig. 2
MycoBank: MB 845477
Etymology:— The epithet “ovatospora” refers to the ovate shape of conidia.
Holotype:— CHINA. Yunnan Province: Jiangcheng County, Jiahe Town, Jiangxi village, 23°13′50′′N, 101°19′13′′E, alt. 880 m, on a termite buried in soil, 15 June 2022, H. Yu (YHH 2206001, holotype; YFCC 22069183, ex-type culture). GenBank accession number: ITS = OP295105, nrSSU = OP295110, nrLSU = OP295113, tef-1a = OP313801, rpb1 = OP313803, rpb2 = OP313805.
Description:—Sexual morph: Stroma solitary, simple, cylindrical or clavate, glabrous, smooth, up to 11–16 cm (x̅ = 13 cm) long, 1–1.2 mm (x̅ = 1 mm) in diam., pale yellow, 9 cm emerging above leaf litter, 4 cm buried in the soil, fertile portion enlarging and generating toward the upper part of stromata, 30–35 × 1.2–1.4 mm (x̅ = 35 × 1.3 mm, n = 2), with perithecial apices. Perithecia pseudo-immersed, ovoid to pyriform, with short, exposed neck or rounded ostiole, (100–)110–140 × (75–)80–110 µm (x̅ = 125 × 95 µm, n = 30). Asci hyaline, filiform, 8-spored, (100–)110–125(–130) × (4–)5–7 µm (x̅ = 120 × 6 µm, n = 30); ascus caps prominent, capitate, 3–4 µm high (x̅ = 4 µm, n = 30) and 4–5 µm wide (x̅ = 4 µm, n = 30). Ascospores whole, hyaline, filiform, multiseptate, (100–)110–130 × 1–2 µm (x̅ = 120 × 2 µm, n = 20). Asexual morph: Hirsutella. Colonies on PDA, 55–60 mm diam. In 30 days at 25 °C, mycelium abundant, cushioned, slightly convex, pale to ivory. Stromata emerging after 10 days, ivory to pale yellow, up to 25–40 × 1–2 mm (x̅ = 30 × 1.5 mm, n = 30), reverse pale yellow to dark purple.Asexual conidiogenous structures were not observed from the stroma. Hyphae hyaline, septate, smooth-walled. Conidiogenous cells arising from aerial mycelium on PDA, monophialidic or rarely polyphialidic, hyaline, smooth, swollen towards the base, flask-shaped, tapering gradually into a thin neck, (10–)15–35(–40) µm (x̅ = 25 µm, n = 20) long, (2–)3–6 µm (x̅ = 5 µm, n = 20) wide at the base, 0.6–1.2 µm (x̅ = 0.8 µm, n = 20) wide at the apex. Conidia solitary, smooth-walled, hyaline, ovate, 3–5 × 3–4 µm (x̅ = 4 × 3.5 µm, n = 20).
Habitat:— On adult termites (Termitidae) buried in soil, emerging from the leaf litter in subtropical monsoon evergreen broad-leaf forest.
Known distribution:— China, Yunnan Province, Jiangcheng County, Jiahe Town, Jiangxi village.
Additional specimen examined:— CHINA. Yunnan Province: Jiangcheng County, Jiahe Town, Jiangxi village, 23°13′50′′N, 101°19′13′′E, alt. 880 m, on a termite buried in soil, 15 June 2022, D.X. Tang (YHH 2206002; YFCC 22069184, living culture). GenBank accession number: ITS = OP295106, nrSSU = OP295111, nrLSU = OP295114, tef-1a = OP313802, rpb1 = OP313804.
Notes:— Ophiocordyceps ovatospora is characterized by solitary pale yellow stromata, pseudo-immersed, ovoid to pyriform perithecia, filiform asci, filiform ascospores, and flask-shaped conidiogenous cells with solitary conidia.
In the phylogenetic analyses, O. ovatospora was clustered in Hirsutella clade of Ophiocordyceps and formed a sister lineage with O. khokpasiensis, with strong statistical support by BP = 100%, PP = 100%. The total sequence length was 5573 bp for O. ovatospora and O. khokpasiensis, the gap sites of O. ovatospora was 463 bp, and O. khokpasiensis was 3785 bp, their conserved sites were 1788 bp, and variable sites were 26 bp. Ophiocordyceps ovatospora is morphologically similar to O. khokpasiensis by producing pseudo-immersed perithecia, filiform asci and ascospores, monophialidic phialides, and oval conidia. However, the two species differ in size of the perithecia, asci and ascospores. Ophiocordyceps ovatospora has smaller perithecia than O. khokpasiensis (110–140 × 80–110 µm vs. 200–250 × 120–200 µm) but larger asci (110–125 × 5–7 µm vs. 62.5–125 × 4–5 µm) (Table 2).
description: Published as part of Tang, Dexiang, Zhu, Juye, Luo, Lijun, Hou, Donghai, Wang, Zhiqin, Yang, Shidao & Yu, Hong, 2022, Ophiocordyceps ovatospora sp. nov. (Ophiocordycipitaceae, Hypocreales), pathogenic on termites from China, pp. 105-117 in Phytotaxa 574 (1) on pages 111-113, DOI: 10.11646/phytotaxa.574.1.8, http://zenodo.org/record/7359863