title: ÐÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ðµ CRISPR-Ð¸Ð½ÑÐµÑÑÐµÑÐµÐ½ÑÐ¸Ð¸ Ð´Ð»Ñ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² ftsA Ð¸ ftsZ Ð² Ð±Ð°ÐºÑÐµÑÐ¸ÑÑ Mycoplasma gallisepticum
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: The use of CRISPR interference to reduce the expression of the ftsA and ftsZ genes in Mycoplasma gallisepticum bacteria
Creators: name: ÐÐ°Ð³Ð¾ÑÐ½ÑÐ¹, ÐÐ¸ÑÐ¸Ð»Ð»
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: CRISPR-Ð¸Ð½ÑÐµÑÑÐµÑÐµÐ½ÑÐ¸Ñ
ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ðµ Ð´ÐµÐ»ÐµÐ½Ð¸Ðµ
CRISPR interference
cell division

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ CRISPR-Ð¸Ð½ÑÐµÑÑÐµÑÐµÐ½ÑÐ¸Ð¸ Ð½Ð° ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð³ÐµÐ½Ð¾Ð² ftsA Ð¸ ftsZ Ð² M. gallisepticum. ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: 1. Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐ°ÑÑ Ð¿ÑÐ¾ÑÐ¾ÐºÐ¾Ð» Ð¿ÑÐ¾Ð±Ð¾Ð¿Ð¾Ð´Ð³Ð¾ÑÐ¾Ð²ÐºÐ¸, Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÑÑÐ¸Ð¹ Ð¿Ð¾Ð»ÑÑÐ°ÑÑ ÐºÑÐ»ÑÑÑÑÑ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð² M. gallisepticum, Ð½Ð°ÑÐ¾Ð´ÑÑÐ¸ÑÑÑ Ð² Ð¾Ð´Ð¸Ð½Ð°ÐºÐ¾Ð²Ð¾Ð¹ ÑÐ°Ð·Ðµ ÑÐ¾ÑÑÐ°; 2. ÐÑÐ´ÐµÐ»Ð¸ÑÑ ÑÐ¾ÑÐ°Ð»ÑÐ½ÑÑ Ð ÐÐ Ð¸Ð· Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ ÐºÑÐ»ÑÑÑÑ; 3. ÐÐ¾Ð»ÑÑÐ¸ÑÑ ÐºÐÐÐ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½Ð¾Ð¹ ÑÐ¾ÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð ÐÐ; 4. Ð¡ÑÐ°Ð²Ð½Ð¸ÑÑ ÑÑÐ¾Ð²Ð½Ð¸ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² ftsA Ð¸ ftsZ Ð² ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ ÑÑÐ°Ð¼Ð¼Ð°Ñ M. gallisepticum, Ð²ÐºÐ»ÑÑÐ°Ñ ÑÑÐ°Ð¼Ð¼Ñ Ñ CRISPR-Ð¸Ð½ÑÐµÑÑÐµÑÐµÐ½ÑÐ¸ÐµÐ¹, Ð¿ÑÐ¸ Ð¿Ð¾Ð¼Ð¾ÑÐ¸ ÐÐ¦Ð  Ð² ÑÐµÐ°Ð»ÑÐ½Ð¾Ð¼ Ð²ÑÐµÐ¼ÐµÐ½Ð¸; Ð Ð°Ð±Ð¾ÑÐ° Ð±ÑÐ»Ð° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ ÐÐ°ÑÑÐ½Ð¾-Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÑÐºÐ¾Ð³Ð¾ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ° Â«ÐÐ°Ð½Ð¾Ð±Ð¸Ð¾ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¸Â» Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¾Ð³Ð¾ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑÐ° ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾. ÐÐ±ÑÐµÐºÑÐ¾Ð¼ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÑÑÑÑ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÐµ ÑÑÐ°Ð¼Ð¼Ñ Ð±Ð°ÐºÑÐµÑÐ¸Ð¸ M. gallisepticum. Ð ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð² Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼ÑÑ ÑÑÐ°Ð¼Ð¼Ð°Ñ CRISPR-Ð¸Ð½ÑÐµÑÑÐµÑÐµÐ½ÑÐ¸Ñ Ð¿Ð¾Ð·Ð²Ð¾Ð»Ð¸Ð»Ð° ÑÐ½Ð¸Ð·Ð¸ÑÑ ÑÑÐ¾Ð²ÐµÐ½Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð° ftsA. Ð ÑÐ¾ Ð¶Ðµ Ð²ÑÐµÐ¼Ñ CRISPR-Ð¸Ð½ÑÐµÑÑÐµÑÐµÐ½ÑÐ¸Ñ Ð½Ðµ Ð¿Ð¾Ð·Ð²Ð¾Ð»Ð¸Ð»Ð° ÑÐ½Ð¸Ð·Ð¸ÑÑ ÑÑÐ¾Ð²ÐµÐ½Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð°Â ftsZ.
description: This work is devoted to determining the effect of CRISPR interference on the expression of the ftsA and ftsZ genes in M. gallisepticum. Tasks that were solved during the research: 1. To develop a sample preparation protocol that allows to obtain cultures of different M. gallisepticum strains in the same growth phase; 2. To isolate total RNA from the obtained cultures; 3. To obtain cDNA based on isolated total RNA; 4. To compare the expression levels of the FtsA and FtsZ genes in various M. gallisepticum strains, including strains with CRISPR interference, using real-time PCR; The work was carried out on the basis of the scientific research complex "Nanobiotechnology" of Peter the Great St. Petersburg Polytechnic University. The object of the study of this work are various strains of the bacterium M. gallisepticum strains. During the study, it was shown that in the studied strains, CRISPR interference allowed to reduce the expression level of the ftsA gene. At the same time, CRISPR interference did not reduce the expression level of the ftsZ gene.