cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
The risk of non-union per fracture: current myths and revised figures from a population of over 4 million adults
Creators: name: Leanora A Mills
name: Aitken, Stuart A
name: A Hamish R W Simpson
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Physiology
subject: Biotechnology
subject: Evolutionary Biology
subject: Ecology
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics

description: Background and purpose — Fracture non-union remains a major clinical problem, yet there are no data available regarding the overall risk of fractures progressing to non-union in a large population. We investigated the rate of non-union per fracture in a large adult population. Methods — National data collected prospectively over a 5-year period and involving just under 5,000 non-unions were analyzed and compared to the incidence of fracture in the same period. Results and interpretation — The overall risk of non-union per fracture was 1.9%, which is considerably less than previously believed. However, for certain fractures in specific age groups the risk of non-union rose to 9%. As expected, these higher rates of non-union were observed with tibial and clavicular fractures, but—less expectedly—it was in the young and middle-aged adults rather than in the older and elderly population. This study is the first to examine fracture non-union rates in a large population according to age and site, and provides more robust (and lower) estimates of non-union risk than those that are frequently quoted.
Syntheses, structural diversity and properties of three coordination polymers built by chlorophenyl imidazole dicarboxylate
Creators: name: Yue, Zhifang
name: Wang, Feng
name: Li, Xianqiang
name: Chen, Zhenna
name: Zhu, Yanyan
name: Li, Gang
Publisher: name: Taylor & Francis
Year: 2015
Subjects: Biological Sciences
Chemistry
Earth and Environmental Sciences
Biotechnology
Medicine
Physics
Biochemistry
Biophysics

description: Two 2D coordiantion polymers, [Sr(p-ClPhHIDC)(H2O)]n (p-ClPhH3IDC = 2-(4-chlorophenyl)-1H-imidazole-4,5-dicarboxylic acid) (1) and [Cd3(p-ClPhH2IDC)2(p-ClPhHIDC)2(H2O)2]n (2) and one 3D polymer {[Co3(p-ClPhHIDC)3(H2O)3]·6H2O}n (3) have been prepared successfully under solvothermal conditions and structurally characterised by elemental analyses, IR and XRPD determinations, and have been further analysed by single-crystal X-ray diffraction. Polymer 1 exhibits a graceful 2D grid sheet structure. Polymer 2 can be regarded as a (3,3,4)-coordinated 2D network. Polymer 3 exhibits a 3D motif with 1D open channels and [Co12(p-ClPhHIDC)9] bihexagonal rings. The coordination features of the newly prepared imidazole dicarboxylate ligand, p-ClPhH3IDC, have been discussed from both theoretical and experimental aspects. Furthermore, the solid-state photoluminescence and thermal properties of the polymers have also been investigated.
Algorithms Don’t Have A Future: On the Relation of Judgement and Calculation
Creators: name: Stader, Daniel
Publisher: name: Freie Universität Berlin
Year: 2024
Subjects: subject: judgement
subject: calculation
subject: algorithms
subject: AI
subject: hermeneutics
subject: prejudice
subject: artificial neural networks
subject: 100 Philosophie und Psychologie::100 Philosophie::102 Verschiedenes

description: This paper is about the opposite of judgement and calculation. This opposition has been a traditional anchor of critiques concerned with the rise of AI decision making over human judgement. Contrary to these approaches, it is argued that human judgement is not and cannot be replaced by calculation, but that it is human judgement that contextualises computational structures and gives them meaning and purpose. The article focuses on the epistemic structure of algorithms and artificial neural networks to find that they always depend on human judgement to be related to real life objects or purposes. By introducing the philosophical concept of judgement, it becomes clear that the property of judgement to provide meaning and purposiveness is based on the temporality of human life and the ambiguity of language, which quantitative processes lack. A juxtaposition shows that calculations and clustering can be used and referred to in more or less prejudiced and reflecting as well as opaque and transparent ways, but thereby always depend on human judgement. The paper clearly asserts that the transparency of AI is necessary for their autonomous use. This transparency requires the explicitness of the judgements that constitute these computational structures, thereby creating an awareness of the conditionality of such epistemic entities.
A longitudinal study of morning, evening, and night light intensities and nocturnal sleep quality in a working population
Creators: name: Amdisen, Lau
name: Daugaard, Stine
name: Vestergaard, Jesper Medom
name: Vested, Anne
name: Bonde, Jens Peter
name: Vistisen, Helene Tilma
name: Christoffersen, Jens
name: Garde, Anne Helene
name: Hansen, Åse Marie
name: Markvart, Jakob
name: Schlünssen, Vivi
name: Kolstad, Henrik Albert
Publisher: name: Taylor & Francis
Year: 2021
Subjects: subject: Neuroscience
subject: Physiology
subject: FOS: Biological sciences
subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: Science Policy
subject: 111714 Mental Health
subject: FOS: Health sciences

description: We aimed to investigate whether higher light intensity in the morning is associated with better nocturnal sleep quality and whether higher light intensities in the evening or night have the opposite effect. Light intensity was recorded for 7 consecutive days across the year among 317 indoor and outdoor daytime workers in Denmark (55–56° N) equipped with a personal light recorder. Participants reported sleep quality after each nocturnal sleep. Sleep quality was measured using three parameters; disturbed sleep index, awakening index, and sleep onset latency. Associations between increasing light intensities and sleep quality were analyzed using mixed effects models with participant identity as a random effect. Overall, neither white nor blue light intensities during morning, evening, or night were associated with sleep quality, awakening, or sleep onset latency of the subsequent nocturnal sleep. However, secondary analyses suggested that artificial light during the morning and day contrary to solar light may increase vulnerability to evening light exposure. Altogether, we were not able to confirm that higher morning light intensity significantly improves self-reported sleep quality or that higher evening or night light intensities impair self-reported sleep quality at exposure levels encountered during daily life in a working population in Denmark. This suggests that light intensities alone are not important for sleep quality to a degree that it is distinguishable from other important parameters in daily life settings.
Temporal and spatial intraspecific variation of primary seed dispersal in Scrophularia canina L., a widespread plant with unspecialised diaspores
Creators: name: Rodríguez-Riaño, Tomás
name: Pérez-Bote, José L.
name: López, Josefa
name: Valtueña, Francisco J.
name: González, Miguel
name: Ortega-Olivencia, Ana
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Medicine
subject: Ecology
subject: FOS: Biological sciences
subject: Plant Biology
subject: 60506 Virology

description: Background: As seed dispersal can vary among years and individuals, studies that focus on a single year or on a few individuals may lead to erroneous conclusions. Aims: To study temporal and spatial intraspecific variation of seed dispersal in Scrophularia canina, a widespread species with capsule-type fruit. Methods: Primary seed dispersal was quantified by placing traps in each cardinal direction around 10 individuals during two consecutive years. We correlated several seed shadow parameters (modal dispersal distance, kurtosis, skewness, percentiles, slope, and seed percentage beneath the plant canopy) with three plant features (maximum height, lateral spread and seed production). Results: Scrophularia canina dispersed their seeds by boleochory, giving rise to a typical leptokurtic curve, but behaving as a barochorous species, because about 90% of seeds landed beneath the plant canopy. Temporal dispersal in S. canina included several seed waves associated with maximum wind speeds. Plant lateral spread was significantly positively correlated with seed percentiles and percentage of seeds beneath the plant canopy regardless of year. A seed production effect was only evident when both years were considered together. Conclusions: Although time-consuming, investigation of the dispersal process for more than 1 year provides more realistic information on seed dispersal. Lateral spread is the main plant feature determining seed shadow.
Ethical issues in video games-Interview study
Creators: name: Aslam, Kousar
Publisher: name: figshare
Year: 2025
Subjects: classificationCode: 461202
subject: Empirical software engineering

description: We conducted semi-structured interviews with 31 software practitioners working in design, development, and production roles within the game industry, recruited through purposive and snowball samplingstrategies. The interview data was analyzed using thematic analysis with an inductive coding approach. Participants identified several ethical issues in game development with gender, racial/cultural, and LGBTQ+ representation emerging as the most prominent. Developers cited commercial pressures, team homogeneity, tight timelines, and inadequate reporting mechanisms as major barriers to ethicalimplementation. Diversifying teams and implementing anonymous reporting channels were proposed to improve the current situation. Our findings reveal the urgency of addressing ethical concerns in game development. Systemic changes are needed in organizational culture, ranging from introducingclear ethical guidelines to ensuring inclusive team structures and organizing ethics-based design and development training.
Application of 233 nm far-UVC LEDs for eradication of MRSA and MSSA and risk assessment on skin models
Creators: name: Zwicker, Paula
name: Schleusener, Johannes
name: Lohan, Silke B.
name: Busch, Loris
name: Sicher, Claudia
name: Einfeldt, Sven
name: Kneissl, Michael
name: Kühl, Anja A.
name: Keck, Cornelia M.
name: Witzel, Christian
name: Kramer, Axel
name: Meinke, Martina C.
Publisher: name: Charité - Universitätsmedizin Berlin
Year: 2022
Subjects: subject: UVC LED source
subject: wavelength of 233 nm
subject: bactericidal efficacy
subject: skin tolerability
subject: 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit

description: A newly developed UVC LED source with an emission wavelength of 233 nm was proved on bactericidal efficacy and skin tolerability. The bactericidal efficacy was qualitatively analysed using blood agar test. Subsequently, quantitative analyses were performed on germ carrier tests using the MRSA strain DSM11822, the MSSA strain DSM799, S. epidermidis DSM1798 with various soil loads. Additionally, the compatibility of the germicidal radiation doses on excised human skin and reconstructed human epidermis was proved. Cell viability, DNA damage and production of radicals were assessed in comparison to typical UVC radiation from discharge lamps (222 nm, 254 nm) and UVB (280-380 nm) radiation for clinical assessment. At a dose of 40 mJ/cm(2), the 233 nm light source reduced the viable microorganisms by a log(10) reduction (LR) of 5 log(10) levels if no soil load was present. Mucin and protein containing soil loads diminished the effect to an LR of 1.5-3.3. A salt solution representing artificial sweat (pH 8.4) had only minor effects on the reduction. The viability of the skin models was not reduced and the DNA damage was far below the damage evoked by 0.1 UVB minimal erythema dose, which can be regarded as safe. Furthermore, the induced damage vanished after 24 h. Irradiation on four consecutive days also did not evoke DNA damage. The radical formation was far lower than 20 min outdoor visible light would cause, which is classified as low radical load and can be compensated by the antioxidant defence system.
The emerging significance of circadian rhythmicity in microvascular resistance
Creators: name: Kroetsch, Jeffrey T.
name: Lidington, Darcy
name: Bolz, Steffen-Sebastian
Publisher: name: Taylor & Francis
Year: 2021
Subjects: subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Neuroscience
subject: Physiology
subject: Biotechnology
subject: Evolutionary Biology
subject: 69999 Biological Sciences not elsewhere classified
subject: Cancer
subject: 111714 Mental Health
subject: FOS: Health sciences
subject: 110309 Infectious Diseases

description: The Earth’s rotation generates environmental oscillations (e.g., in light and temperature) that have imposed unique evolutionary pressures over millions of years. Consequently, the circadian clock, a ubiquitously expressed molecular system that aligns cellular function to these environmental cues, has become an integral component of our physiology. The resulting functional rhythms optimize and economize physiological performance: perturbing these rhythms, therefore, is frequently deleterious. This perspective article focuses on circadian rhythms in resistance artery myogenic reactivity, a key mechanism governing tissue perfusion, total peripheral resistance and systemic blood pressure. Emerging evidence suggests that myogenic reactivity rhythms are locally generated in a microvascular bed-specific manner at the level of smooth muscle cells. This implies that there is a distinct interface between the molecular clock and the signalling pathways underlying myogenic reactivity in the microvascular beds of different organs. By understanding the precise nature of these molecular links, it may become possible to therapeutically manipulate microvascular tone in an organ-specific manner. This raises the prospect that interventions for vascular pathologies that are challenging to treat, such as hypertension and brain malperfusion, can be significantly improved.
New Zn(II) complexes based on biologically active substituted acetic acid and nitrogen donor ligands: synthesis, crystal structure and biological applications
Creators: name: Shayma Kamel
name: Hijazi Abu Ali
name: Shamma, Amani Abu
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Science Policy
subject: Immunology
subject: FOS: Clinical medicine

description: The complexes [Zn(phenylacetato)2(2-aminopyridin)2] (3), [Zn(phenylacetato)2(1,10-phenanthroline)]·H2O (4), and [Zn(phenylacetato)2(2,9-dimethyl-1,10-phenanthroline)]·0.5 H2O (5) were prepared and characterized by IR-, UV–Visible, 1H and 13C NMR spectroscopy, and single crystal X-ray diffraction. BNPP hydrolysis of the complexes and their parent nitrogen ligands showed that the hydrolysis rate of bis-(4-nitrophenyl) phosphate (BNPP) was 1.7 × 105 L mol−1 s−1 for 3, 3.1 × 105 L mol−1 s−1 for 4 and 4.3 × 104 L mol−1 s−1 for 5. Antibacterial activities show the effect of complexation on activity against Gram-positive (S. epidermidis, S. aureus, E. faecalis, M. luteus and B. subtilis) and Gram-negative (K. pneumonia, E. coli, P. mirabilis and P. aeruginosa) bacteria using the agar well diffusion method. Complex 4 showed good activity against G− bacteria except P. aeruginosa, and against G+ bacteria except E. ferabis. Complex 5 showed no activity against G− bacteria, low activity against M. luteus and B. subtilis bacteria and high activity against S. epidemidis and S. aureus. Complex 3 did not show any activity against G− or G+ bacteria.
Climate impacts from road bridges: effects of introducing concrete carbonation and biogenic carbon storage in wood
Creators: name: Peñaloza, Diego
name: Erlandsson, Martin
name: Pousette, Anna
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Biophysics
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Science Policy
subject: Plant Biology

description: The construction sector faces the challenge of mitigating climate change with urgency. Life cycle assessment (LCA), a widely used tool to assess the climate impacts of buildings, is seldom used for bridges. Material-specific phenomena such as concrete carbonation and biogenic carbon storage are usually unaccounted for when assessing the climate impacts from infrastructure. The purpose of this article is to explore the effects these phenomena could have on climate impact assessment of road bridges and comparisons between bridge designs. For this, a case study is used of two functionally equivalent design alternatives for a small road bridge in Sweden. Dynamic LCA is used to calculate the effects of biogenic carbon storage, while the Lagerblad method and literature values are used to estimate concrete carbonation. The results show that the climate impact of the bridge is influenced by both phenomena, and that the gap between the impacts from both designs increases if the phenomena are accounted for. The outcome is influenced by the time occurrence assumed for the forest carbon uptake and the end-of-life scenario for the concrete. An equilibrium or 50/50 approach for accounting for the forest carbon uptake is proposed as a middle value compromise to handle this issue.
An exploratory study of associations between sleep timing variability and cardiometabolic health in middle-aged adults with type 2 diabetes mellitus
Creators: name: Kelly, Rachael M.
name: Healy, Ultan
name: Sreenan, Seamus
name: McDermott, John
name: Coogan, Andrew N.
Publisher: name: Taylor & Francis
Year: 2022
Subjects: subject: Biochemistry
subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Physiology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: 111714 Mental Health
subject: FOS: Health sciences

description: Sleep is increasingly recognised as an important risk factor for metabolic disease, and as an important influence on severity in established metabolic disease. Recent evidence suggests that sleep timing variability (the day-to-day fluctuations of sleep timing) may be an important factor in metabolic diseases such as type 2 diabetes mellitus. In the current study, we explore the associations between measures of sleep timing variability and cardiometabolic measures in a group of healthy middle-aged adults with and without type 2 diabetes mellitus. Healthy controls (N = 27) and adults with well-controlled uncomplicated type 2 diabetes mellitus (N = 30) wore actiwatches for an average of 9 days for objective assessment of sleep timing parameters, and also underwent a detailed clinical assessment. We found greater self-reported social jetlag in the diabetes group, but no groupwise differences in measures of sleep timing variability. In the diabetes patients, HbA1c levels were inversely correlated with variability in the time of sleep onset and midsleep, and with sleep duration. HOMA-IR did not correlate with any sleep timing variability measure, nor were there associations between sleep timing variability and other metabolic biomarkers (cholesterol, LDL, HDL, triglycerides and uric acid). Systolic blood pressure was inversely correlated with actigraphically defined social jetlag in both the control and diabetes groups. The results of this study indicate associations between sleep timing variability and HbA1c, but the direction of these relationships is at variance with some other recent reports. Our results indicate a need for future hypothesis-testing studies to further explore the impact of sleep timing variance on metabolic health.
Fully automated quantification of in vivo viscoelasticity of prostate zones using magnetic resonance elastography with Dense U-net segmentation
Creators: name: Aldoj, Nader
name: Biavati, Federico
name: Dewey, Marc
name: Hennemuth, Anja
name: Asbach, Patrick
name: Sack, Ingolf
Publisher: name: Charité - Universitätsmedizin Berlin
Year: 2022
Subjects: subject: prostate zones
subject: Magnetic resonance elastography (MRE)
subject: Dense U-net segmentation
subject: 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit

description: Magnetic resonance elastography (MRE) for measuring viscoelasticity heavily depends on proper tissue segmentation, especially in heterogeneous organs such as the prostate. Using trained network-based image segmentation, we investigated if MRE data suffice to extract anatomical and viscoelastic information for automatic tabulation of zonal mechanical properties of the prostate. Overall, 40 patients with benign prostatic hyperplasia (BPH) or prostate cancer (PCa) were examined with three magnetic resonance imaging (MRI) sequences: T2-weighted MRI (T2w), diffusion-weighted imaging (DWI), and MRE-based tomoelastography, yielding six independent sets of imaging data per patient (T2w, DWI, apparent diffusion coefficient, MRE magnitude, shear wave speed, and loss angle maps). Combinations of these data were used to train Dense U-nets with manually segmented masks of the entire prostate gland (PG), central zone (CZ), and peripheral zone (PZ) in 30 patients and to validate them in 10 patients. Dice score (DS), sensitivity, specificity, and Hausdorff distance were determined. We found that segmentation based on MRE magnitude maps alone (DS, PG: 0.93 +/- 0.04, CZ: 0.95 +/- 0.03, PZ: 0.77 +/- 0.05) was more accurate than magnitude maps combined with T2w and DWI_b (DS, PG: 0.91 +/- 0.04, CZ: 0.91 +/- 0.06, PZ: 0.63 +/- 0.16) or T2w alone (DS, PG: 0.92 +/- 0.03, CZ: 0.91 +/- 0.04, PZ: 0.65 +/- 0.08). Automatically tabulated MRE values were not different from ground-truth values (P>0.05). In conclusion, MRE combined with Dense U-net segmentation allows tabulation of quantitative imaging markers without manual analysis and independent of other MRI sequences and can thus contribute to PCa detection and classification.
Development of fluorescence polarisation immunoassay for carbofuran in food and environmental water samples
Creators: name: Yang, Jin-Yi
name: Zhang, Yan
name: Wang, Hong
name: Xu, Zhen-Lin
name: A. Eremin, Sergei
name: Shen, Yu-Dong
name: Wu, Qing
name: Lei, Hong-Tao
name: Sun, Yuan-Ming
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Plant Biology
subject: FOS: Biological sciences
subject: Inorganic Chemistry
subject: FOS: Chemical sciences
subject: Biological Sciences
subject: Ecology
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Cell Biology
subject: Engineering
subject: Biochemistry
subject: Biophysics

description: A simple and rapid method for determination of carbofuran was developed using the fluorescence polarisation immunoassay (FPIA). Three tracers with different lengths of bridge (0-, 2- and 6-carbon bridge) between the hapten molecule 4-[[(2,3-dihydro-2,2-dimethyl-7-benzofuranyloxy)carbonyl]-amino]-butanoic acid (BFNB) and 5-aminofluorescein (AF), fluoresceinthiocarbamyl ethylenediamine (EDF), fluoresceinthiocarbamyl hexylenediamine (HDF), were synthesised and their binding response with anti-carbofuran-specific antibody were evaluated. The physicochemical parameters were optimised for the FPIA. The AF-labelled BFNB conjugate (BFNB-AF) was found to be the optimal tracer for FPIA of carbofuran. The detection limit of carbofuran, IC50 value and the working range were 2.3, 48.8 and 7.4−202.2 µg/L, respectively; and the reaction time was only 10 min. The average recovery from spiked water and vegetable samples was 86.9−95.4% and the mean coefficient of variation was 6.2% for inter-assay and 8.7% for intra-assay, which showed good reproducibility for FPIA. Thus, the developed FPIA method exhibited the potential for the rapid and accurate determination of carbofuran in agricultural and environmental samples.
Fact Sheet: Biodiversity
Creators: name: The Nansen Legacy
Publisher: name: Zenodo
Year: 2024

description: This fact sheet is a deliverable of the project The Nansen Legacy, and it brings together core findings of the project. 

Scientific observations of many Nansen Legacy researchers form the background for this fact sheet. The following researchers have summarized the findings and provided the text on the fact sheet: B. Bluhm (lead), T.M. Gabielsen, C. Gawinski, È. Jordà Molina, K. Saubrekka, J. Vikberg Wernström (alphabetical order, apart from lead scientist).

All illustrations and the design of the fact sheet have been made by F.A. Cnossen.
ALGORITMLAR TUSHUNCHASI VA ULARDAN FOYDALANISH
Creators: name: Xaydarova, Ruxshona
name: Mamasoliyeva, Maxsuda
name: Gurgunboyeva, Munisa
Publisher: name: Zenodo
Year: 2025

description: Bugungi kunda algoritmlar hayotimizning ajralmas qismiga aylanishga ulgurgan.Har kuni algoritmlar yordamida ko’plab muommolarimizni hal qilamiz.Masalan, internetdan yoki turli dasturlardan foydalanishda ishlatishimiz mumkin.Algoritm nima,turlari,u qanday ishlashi va hayotimizdagi roli haqida ko’rib chiqamiz.
Role of TRPC6 in kidney damage after acute ischemic kidney injury
Creators: name: Zheng, Zhihuang
name: Tsvetkov, Dmitry
name: Bartolomaeus, Theda Ulrike Patricia
name: Erdogan, Cem
name: Krügel, Ute
name: Schleifenbaum, Johanna
name: Schaefer, Michael
name: Nürnberg, Bernd
name: Chai, Xiaoning
name: Ludwig, Friedrich-Alexander
name: N’diaye, Gabriele
name: Köhler, May-Britt
name: Wu, Kaiyin
name: Gollasch, Maik
name: Markó, Lajos
Publisher: name: Charité - Universitätsmedizin Berlin
Year: 2022
Subjects: subject: Transient receptor potential channel subfamily C, member 6 (TRPC6)
subject: chronic kidney disease
subject: acute kidney injury (AKI)
subject: 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit

description: Transient receptor potential channel subfamily C, member 6 (TRPC6), a non-selective cation channel that controls influx of Ca2+ and other monovalent cations into cells, is widely expressed in the kidney. TRPC6 gene variations have been linked to chronic kidney disease but its role in acute kidney injury (AKI) is unknown. Here we aimed to investigate the putative role of TRPC6 channels in AKI. We used Trpc6(-/-) mice and pharmacological blockade (SH045 and BI-749327), to evaluate short-term AKI outcomes. Here, we demonstrate that neither Trpc6 deficiency nor pharmacological inhibition of TRPC6 influences the short-term outcomes of AKI. Serum markers, renal expression of epithelial damage markers, tubular injury, and renal inflammatory response assessed by the histological analysis were similar in wild-type mice compared to Trpc6(-/-) mice as well as in vehicle-treated versus SH045- or BI-749327-treated mice. In addition, we also found no effect of TRPC6 modulation on renal arterial myogenic tone by using blockers to perfuse isolated kidneys. Therefore, we conclude that TRPC6 does not play a role in the acute phase of AKI. Our results may have clinical implications for safety and health of humans with TRPC6 gene variations, with respect to mutated TRPC6 channels in the response of the kidney to acute ischemic stimuli.
Supplementary Material_PROCB2 from A fossil protein chimera; difficulties in discriminating dinosaur peptide sequences from modern cross-contamination
Creators: name: Buckley, Michael
name: Warwood, Stacey
name: Kitchener, Andrew C.
name: Manning, Phillip L.
Publisher: name: The Royal Society
Year: 2017
Subjects: subject: Biochemistry
subject: Evolutionary Biology
subject: FOS: Biological sciences
subject: Environmental Science

description: A decade ago, reports that organic-rich soft tissue survived from dinosaur fossils were apparently supported by proteomics-derived sequence information of exceptionally well-preserved bone. This initial claim to the sequencing of endogenous collagen peptides from an approximately 68-Myr Tyrannosaurus rex fossil was highly controversial, largely on the grounds of potential contamination from either bacterial biofilms or from laboratory practice. In a subsequent study, collagen peptide sequences from an approximately 78 Myr Brachylophosaurus canadensis fossil were reported which has remained largely unchallenged. However, the endogeneity of these sequences relies heavily on a single peptide sequence, apparently unique to both dinosaurs. Given the potential for cross-contamination from modern bone analysed by the same team, here we extract collagen from bone samples of three individuals of ostrich, Struthio camelus. The resulting LC–MS/MS data were found to match all of the proposed sequences for both the original Tyrannosaurus and Brachylophosaurus studies. Regardless of the true nature of the dinosaur peptides, our finding highlights the difficulty of differentiating such sequences with confidence. Our results not only imply that cross-contamination cannot be ruled out, but that appropriate measures to test for endogeneity should be further evaluated.
A dimensão política do jornalismo
Creators: name: Serrano, Estrela
Publisher: name: Comunicação & Cultura
Year: 2006

description: Partindo de uma revisão da literatura europeia e norte-americana sobre a dimensão política do jornalismo, o artigo analisa as relações entre o campo político e o campo do jornalismo, nomeadamente a interacção entre jornalistas e fontes, as práticas jornalísticas em diferentes contextos políticos e a intersecção do jornalismo com o mundo político e com as audiências. Através de uma selecção de artigos de opinião publicados na imprensa portuguesa entre 2000 e 2005, da autoria de figuras públicas relevantes no campo político nacional, o artigo pretende mostrar que a relação simbiótica e problemática entre jornalismo e política não é apenas um fenómeno anglo-saxónico ou das democracias avançadas.
description: Comunicação & Cultura, n. 2 (2006): Lobbying e marketing político
Samarium(II)-Promoted Cyclizations of Nonactivated Indolyl Sulfinyl Imines to Polycyclic Tertiary Carbinamines
Creators: name: Nageswara Rao, Chintada
name: Reissig, Hans-Ulrich
Publisher: name: Freie Universität Berlin
Year: 2022
Subjects: subject: Amine
subject: Cyclization
subject: Electron transfer
subject: Indole
subject: Samarium diiodide
subject: 500 Naturwissenschaften und Mathematik::540 Chemie::547 Organische Chemie

description: Samarium(II)-promoted cyclizations of N-acylated indolyl sulfinyl imines without electron-withdrawing groups at C-3 furnished tertiary carbinamines in good yield. Screening of the reaction conditions revealed that application of an excess of samarium diiodide in the presence of water and lithium bromide provided the cleanest reactions and the highest yields. The most striking observation during this investigation was the reductive detachment of the sulfur functional group, which most likely precedes the cyclization step. As consequence no enantioselectivity could be observed if enantiopure sulfinyl imines were employed. The mechanisms of the N−S cleavage and of the cyclization of the intermediate imines as well as the role of the additives are discussed. The presented method generates interesting polycyclic indoline derivatives; a cascade reaction involving an ethoxycarbonyl-substituted side-chain provided unique tetracyclic spiro-γ-lactams.
Analysis of adherent cell culture lysates with low metabolite concentrations using the Biocrates AbsoluteIDQ p400 HR kit
Creators: name: Fritsche-Guenther, Raphaela
name: Gloaguen, Yoann
name: Eisenberger, Alina
name: Kirwan, Jennifer A.
Publisher: name: Charité - Universitätsmedizin Berlin
Year: 2022
Subjects: subject: AbsoluteIDQ p400 HR kit
subject: metabolomics
subject: adherent cell lysates
subject: 600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit

description: The AbsoluteIDQ p400 HR kit is a commercial product for targeted metabolomics. While the kit has been validated for human plasma and serum, adherent cell lysates have not yet been evaluated. We have optimized the detection of polar and lipid metabolites in cell lysates using the kit to enable robust and repeatable analysis of the detected metabolites. Parameters optimized include total cell mass, loading volume and extraction solvent. We present a cell preparation and analytical method and report on the performance of the kit with regard to detectability of the targeted metabolites and their repeatability. The kit can be successfully used for a relative quantification analysis of cell lysates from adherent cells although validated only for human plasma and serum. Most metabolites are below the limit of the Biocrates' set quantification limits and we confirmed that this relative quantification can be used for further statistical analysis. Using this approach, up to 45% of the total metabolites in the kit can be detected with a reasonable analytical performance (lowest median RSD 9% and 13% for LC and FIA, respectively) dependent on the method used. We recommend using ethanol as the extraction solvent for cell lysates of osteosarcoma cell lines for the broadest metabolite coverage and 25 mg of cell mass with a loading volume of 20 mu L per sample.
A mensagem política na campanha das eleições presidenciais: análise de conteúdo dos slogans entre 1976 e 2006
Creators: name: Santo, Paula Do Espírito
Publisher: name: Comunicação & Cultura
Year: 2006

description: O estudo presente tem como objectivo a análise das representações discursivas e políticas, bem como dos valores contidos nos slogans das eleições presidenciais portuguesas, a partir de 1976, data das primeiras eleições presidenciais pós-revolução de 1974, até 2006. Os resultados do estudo incidirão na análise da diferenciação discursiva, política, ideológica e nos valores contidos nos slogans de campanha. Do ponto de vista metodológico, este estudo utiliza a análise de conteúdo, suportada na vertente qualitativa e inferencial, direccionada para os conteúdos informacionais considerados.
description: Comunicação & Cultura, n. 2 (2006): Lobbying e marketing político
Helicity-Sensitive Plasmonic Terahertz Interferometer
Creators: name: Matyushkin, Yakov
name: Danilov, Sergey
name: Moskotin, Maxim
name: Belosevich, Vsevolod
name: Kaurova, Natalia
name: Rybin, Maxim
name: Obraztsova, Elena D.
name: Fedorov, Georgy
name: Gorbenko, Ilya
name: Kachorovskii, Valentin
name: Ganichev, Sergey
Publisher: name: Universität Regensburg
Year: 2020
Subjects: subject: plasmonic interferometer, terahertz radiation, radiation helicity, graphene
subject: 530 Physik

description: Plasmonic interferometry is a rapidly growing area of research with a huge potential for applications in the terahertz frequency range. In this Letter, we explore a plasmonic interferometer based on graphene field effect transistor connected to specially designed antennas. As a key result, we observe helicity- and phase-sensitive conversion of circularly polarized radiation into dc photovoltage caused by the plasmon-interference mechanism: two plasma waves, excited at the source and drain part of the transistor, interfere inside the channel. The helicity-sensitive phase shift between these waves is achieved by using an asymmetric antenna configuration. The dc signal changes sign with inversion of the helicity. A suggested plasmonic interferometer is capable of measuring the phase difference between two arbitrary phase-shifted optical signals. The observed effect opens a wide avenue for phase-sensitive probing of plasma wave excitations in two-dimensional materials.
Co-immobilization of Pseudomonas stutzeri YHA-13 and Alcaligenes sp. ZGED-12 with polyvinyl alcohol–alginate for removal of nitrogen and phosphorus from synthetic wastewater
Creators: name: Han, Yonghe
name: Zhang, Wenxian
name: Lu, Wenxian
name: Zhou, Zhihua
name: Zhuang, Zhigang
name: Li, Min
Publisher: name: Taylor & Francis
Year: 2014
Subjects: Environmental Science

description: Nitrogen (N) and phosphorus (P) are the two main factors causing water eutrophication. Immobilized micro-organisms have been widely studied in N and P removal. However, the effects of various immobilizing conditions on the removal efficiency of N and P using immobilized micro-organism beads (IMOBs) remain unclear. Polyvinyl alcohol (PVA) and alginate, as the two frequently immobilizing-used matrixes, were used for co-immobilizing Pseudomonas stutzeri YHA-13 and Alcaligenes sp. ZGED-12. PVA, alginate and CaCl 2 contents, immobilization time and different wet biomass ratios of P. stutzeri to Alcaligenes sp. were conducted to elucidate their roles in and influences on the removal efficiency of N and P from synthetic wastewater. The application potential of IMOBs was estimated as well. Results showed that IMOBs prepared by cross-link of 4% PVA and 2–3% alginate with 5% CaCl 2 and saturated boric acid solution for 10–15 min are the best ones in removal of N and P. Though IMOBs containing P. stutzeri and/or Alcaligenes sp. were capable of removal of the two nutrients, the highest removal efficiency was observed when the wet biomass ratio of P. stutzeri to Alcaligenes sp. was adjusted to 2:2. In addition, the IMOBs were of good ability to remove chemical oxygen demand (COD), NO 3−, NO 2−, NH 4+− N, total nitrogen (TN) and total phosphorus (TP) from artificial wastewater. Of which, micro-organisms immobilized in matrixes were mainly responsible for NO 3− and TP removal. Therefore, P. stutzeri YHA-13 and Alcaligenes sp. ZGED-12 are reliable bioresources to remove N and P from wastewater.
Tendências de cobertura do final de um ciclo político: Cavaco Silva (1994-1995)
Creators: name: Ferin, Isabel
Publisher: name: Comunicação & Cultura
Year: 2006

description: Este artigo tem como objectivo o estudo da cobertura jornalística do final de mandato do primeiro-ministro Cavaco Silva (1994-1995) nas primeiras páginas dos jornais Diário de Notícias e Público e dos semanários Expresso e O Independente. As hipóteses de trabalho articulam o factor adaptação da imprensa de referência ao novo panorama mediático – decorrente do início de actividades dos operadores privados de televisão – e a literatura internacional que tende a associar a cobertura dos finais de mandatos a períodos difíceis de relacionamento entre os governantes e a imprensa. Para a análise da cobertura recorre-se a metodologias quantitativas de recolha e tratamento de dados (SPSS). A discussão final procura confrontar os resultados obtidos com postulados e factores conjunturais.
description: Comunicação & Cultura, n. 2 (2006): Lobbying e marketing político
title: 01_2178
Creators: name: Wesley Baldwin, John
name: Baldwin, John W.
Publisher: name: NAKALA - https://nakala.fr (Huma-Num - CNRS)
publisherIdentifier: https://ror.org/04ces3204
publisherIdentifierScheme: ROR
Year: 2018
Subjects: subject: LaMOP
subject: Laboratoire de Médiévistique Occidentale de Paris

description: L'ensemble de ces notes ont été crée par John Baldwin lors d'une de ses premières périodes de résidence à Paris. Ces documents comprennent tous les magistri qu’il avait pu rencontrer dans ses sources. Aux XIIème et XIIIème siècles, les universités n'existant pas encore, le titre de magister s'il évoque certainement une compétence n'est pas rattaché de façon systématique au passage par les écoles. De surcroît, les magistris sont le plus souvent désignés par leur seul prénom. La lecture de ces données est donc difficile et suppose un long travail pour tenter d'identifier un individu auquel on pourrait rattacher plusieurs mentions. John Baldwin avait donc renoncé à utiliser ces données pour donner un prolongement à sa magnifique étude sur les gradués d'université et leur emploi par les Capétiens et les Plantagenêts. Néanmoins, il a conservé ces données et elles sont potentiellement très utile dans le cadre de Studium, comme le sont toutes les mentions dans les volumes des Fasti Ecclesiae Gallicanae de chanoines portant ce titre de magister sans autres précisions. L'ampleur des dépouillements rend ces données très utiles et nous devons une grande reconnaissance à John Baldwin pour avoir pris la peine de faire photocopier ses fiches et de nous les avoir transmises amicalement.
Les données ainsi que les images des fiches sont accessibles sur le site Internet à l'adresse suivante : http://studium-baldwin.lamop.fr/.
Les transcriptions des fiches ont été effectuées par des étudiants de Paris 1 Panthéon - Sorbonne mais elles n'ont pas été corrigées par la suite.
description: Relation: https://lamop.hypotheses.org/
Co-immobilization of Pseudomonas stutzeri YHA-13 and Alcaligenes sp. ZGED-12 with PVA-alginate for removal of nitrogen and phosphorus from synthetic wastewater
Creators: name: Han, Yonghe
name: Zhang, Wenxian
name: Lu, Wenxian
name: Zhou, Zhihua
name: Zhuang, Zhigang
name: Li, Min
Publisher: name: Taylor & Francis
Year: 2014
Subjects: Environmental Science

description: Nitrogen (N) and phosphorus (P) are the two main factors causing water eutrophication. Immobilized microorganisms (IMOs) have been widely studied in N and P removal. However, the effects of various immobilizing conditions on the removal efficiency of N and P using immobilized microorganism beads (IMOBs) remain unclear. PVA and alginate, as the two frequently immobilizing-used matrixes, were used for co-immobilizing Pseudomonas stutzeri YHA-13 and Alcaligenes sp. ZGED-12. PVA, alginate and CaCl2 contents, immobilization time and different wet biomass ratios of P. stutzeri to Alcaligenes sp. were conducted to elucidate their roles in and influences on removal efficiency of N and P from synthetic wastewater. The application potential of IMOBs was estimated as well. Results showed that IMOBs prepared by cross-link of 4.0% PVA and 2.0%∼3% alginate with 5.0% CaCl2 and saturated boric acid solution for 10∼15 min are the best one in removal of N and P. Though IMOBs containing P. stutzeri and/or Alcaligenes sp. were capable of removal of the two nutrients, the highest removal efficiency was observed when the wet biomass ratio of P. stutzeri to Alcaligenes sp. was adjusted to 2:2. In addition, the IMOBs were of good ability to remove COD, NO3-, NO2-, NH4+-N, TN and TP from artificial wastewater (AWW). Of which, microorganisms immobilized in matrixes were mainly responsible for NO3- and TP removal. Therefore, P. stutzeri YHA-13 and Alcaligenes sp. ZGED-12 are reliable bioresources to remove N and P from wastewater.
O caso ICN: convergência entre jornalistas e fontes
Creators: name: Pereira, Gonçalo
Publisher: name: Comunicação & Cultura
Year: 2006

description: O relacionamento entre jornalistas e fontes de informação não obedece necessariamente a um modelo social rígido, pautado por antagonismo e interesses divergentes. A partilha de quadros de referência pode gerar práticas de cooperação em que o jornalista e a fonte se assumem como elos da mesma cadeia, interessados no mesmo desfecho, militantes da mesma causa. Em Outubro de 2003, veiculou-se em alguns meios de comunicação portugueses a intenção governamental de extinção do Instituto da Conservação da Natureza (ICN) e a integração das suas competências em organismos já existentes. Para o movimento ambientalista, a medida seria um retrocesso nas pretensões de institucionalização política. O estudo de caso que segue analisa o papel de alguns agentes envolvidos na divulgação pública da notícia, observado pelo autor no decurso de uma investigação mais ampla sobre a consolidação de uma associação ambientalista portuguesa.
description: Comunicação & Cultura, n. 2 (2006): Lobbying e marketing político
Influence of undercompensation parameter, matching strength and niche shape on defaunation curves from Defaunation effects on plant recruitment depend on size matching and size trade-offs in seed-dispersal networks
Creators: name: Donoso, Isabel
name: Schleuning, Matthias
name: García, Daniel
name: Fründ, Jochen
Publisher: name: The Royal Society
Year: 2017
Subjects: subject: Ecology
subject: FOS: Biological sciences
subject: 60102 Bioinformatics
subject: FOS: Computer and information sciences
subject: 10202 Biological Mathematics
subject: FOS: Mathematics

description: Versions of Fig.2-4 after selecting different values for the undercompensation parameter (Fig.S1-S6), specialization parameter (Fig.S7-S12), and a different niche shape (Fig.S13-S15).
Removal of para-nitrochlorobenzene from aqueous solution on surfactant-modified nanoscale zero-valent iron/graphene nanocomposites
Creators: name: Wu, Yan
name: Luo, Hanjin
name: Wang, Hou
Publisher: name: Taylor & Francis
Year: 2014
Subjects: Environmental Science

description: This study demonstrated a remarkably simple and efficient method for the synthesis of nanoscale zero-valent iron (NZVI)/graphene (GN) nanocomposites. In order to prevent the agglomeration and restack of nanocomposites, chemical functionalization of nanocomposites with cetyltrimethylammonium bromide was proposed. The adsorption performance of surfactant-modified NZVI/GN nanocomposites was evaluated for the removal of para-nitrochlorobenzene (p-NCB) from aqueous solutions. The characteristics of nanocomposites were characterized by X-ray diffraction, BET surface area, Fourier transform infrared spectrum, thermogravimetric analysis and scanning electron microscopy. The effect factors including initial solution pH, contact time, reaction temperature, dosage, initial concentration of humic acid (HA) on the adsorption property of p-NCB onto surfactant-modified nanocomposites were investigated. The adsorption kinetics fitted well with pseudo-second-order model. The adsorption capacity of p-NCB on surfactant-modified nanocomposites inferred from the Langmuir model was 105.15 mg/g at 293 K. The thermodynamic parameters indicated that the adsorption of p-NCB onto surfactant-modified nanocomposites was an exothermic and spontaneous process. HA had a strong suppression effect on p-NCB uptake in the adsorption experiment.