Additional file 1: Figure S1. of Long-term persisting hybrid swarm and geographic difference in hybridization pattern: genetic consequences of secondary contact between two Vincetoxicum species (Apocynaceae–Asclepiadoideae)
Creators: name: Li, Yue
name: Fumito Tada
name: Yamashiro, Tadashi
name: Maki, Masayuki
Publisher: name: Figshare
Year: 2016
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Evolutionary Biology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: FOS: Earth and related environmental sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: Inorganic Chemistry
subject: FOS: Chemical sciences
subject: FOS: Chemical sciences
subject: Plant Biology
subject: Computational Biology

description: Map showing presence data for V. atratum (filled purple circles) and V. japonicum (filled yellow circles) populations used in niche modelling. Figure S2. Box-plots showing inter- and infra-population variation distribution in nine morphology character in each population. Rectangles define 25 % and 75 % quartiles (lower and upper edges of the box). Horizontal bar within box present the median. Whiskers extend up to the 90th percentiles and down to the 10th percentiles. Closed circle show outliers. Figure S3. Bar plot showing the inter- and infra-population variation pattern of hair density on leaf and corolla surface. Figure S4. Bayesian inference of the most likely number of clusters in the STRUCTURE analysis. (a) Plot of mean likelihood logarithmic probability of the data using 10 replicates runs at each value of K (K= 1-10). (b) Distribution of delta K for each K estimated from following Evanno et al. (2005). Figure S5. Deviance Index Criterion (DIC) for TESS analyses. Table S1. Details of the sample locations, sample size (N) and genetic diversity measure (N a: mean number of alleles per locus, H E: expected heterozygosity, H O: observed heterozygosity, AR: allele richness, PAR: private allele richness). Table S2. Description and references for the 10 nuclear SSR loci employed in this study. Table S3. Presence data used in niche modelling. Table S4. Bioclimatic variables obtained from the WorldClim and the variables used for predicting the distribution of V. atratum and V. japonicum. Table S5. Pollen fertility examined in the studied Vincetoxicum populations. Percentages of fertile pollen grains per population expressed as minimum, maximum and median. Table S6. Inbreeding coefficient for all populations at each microsatellite loci. Table S7. Linkage disequilibrium between all pairs of loci in each population. Significant P-values are given in bold after Bonferroni corrections (Rice 1989). Table S8. Pairwise estimates of genetic differentiation (F ST) between populations base on 10 microsatellite markers. Table S9. Assignment individuals based on result of STRUCTURE. Table S10. Estimates of gene flow (4Nm) among ‘pure’ V. atratum (A01-A07 and A09), ‘pure’ V. japonicum (J01-J08), introgressed V. japonicum (J09-J11), north group of putative hybrid populations (H01-H05) and south group of putative hybrid populations (H06 and H07). Source populations are listed by column, recipient populations listed by row. (DOCX 928 kb)