title: ÐÐ½Ð°Ð»Ð¸Ð· ÑÐ»ÐµÐºÑÑÐ¾Ð³ÐµÐ½ÐµÐ·Ð° ÐºÐ°ÑÐ´Ð¸Ð¾Ð¼Ð¸Ð¾ÑÐ¸ÑÐ¾Ð² Ð¶Ð¸Ð²Ð¾ÑÐ½Ð¾Ð¹ Ð¼Ð¾Ð´ÐµÐ»Ð¸ ÐºÑÐ°ÑÐºÐ¾ÑÑÐ¾ÑÐ½Ð¾Ð³Ð¾ Ð´Ð¸Ð°Ð±ÐµÑÐ° Ð¿ÐµÑÐ²Ð¾Ð³Ð¾ ÑÐ¸Ð¿Ð°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Analysis of electrogenesis of cardiomyocytes in an animal model of short-term type I diabetes mellitus
Creators: name: ÐÐµÑÑÑÑÐºÐ¾Ð²Ð°, ÐÐ°Ð´Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: ÑÐ°Ñ Ð°ÑÐ½ÑÐ¹ Ð´Ð¸Ð°Ð±ÐµÑ
Ð´Ð¸Ð°Ð±ÐµÑÐ¸ÑÐµÑÐºÐ°Ñ ÐºÐ°ÑÐ´Ð¸Ð¾Ð¼Ð¸Ð¾Ð¿Ð°ÑÐ¸Ñ
ÐºÐ°ÑÐ´Ð¸Ð¾Ð¼Ð¸Ð¾ÑÐ¸ÑÑ
Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ñ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ
Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ð½ÑÐ¹ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»
diabetes mellitus
diabetic cardiomyopathy
cardiomyocytes
action potentials
membrane potential

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð³ÐµÐ½ÐµÑÐ°ÑÐ¸Ð¸ Ð¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð¾Ð² Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ ÑÑÐ±ÑÐ¿Ð¸ÐºÐ°ÑÐ´Ð¸Ð°Ð»ÑÐ½ÑÑ ÐºÐ°ÑÐ´Ð¸Ð¾Ð¼Ð¸Ð¾ÑÐ¸ÑÐ¾Ð² Ð»ÐµÐ²Ð¾Ð³Ð¾ Ð¶ÐµÐ»ÑÐ´Ð¾ÑÐºÐ° Ð¸Ð·Ð¾Ð»Ð¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ ÑÐµÑÐ´ÑÐ° ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½ÑÑ ÐºÑÑÑ Ð¸ ÐºÑÑÑ ÑÐ¾ ÑÑÑÐµÐ¿ÑÐ¾Ð·Ð¾ÑÐ¾ÑÐ¸Ð½-Ð²ÑÐ·Ð²Ð°Ð½Ð½ÑÐ¼ ÑÐ°ÑÐ°ÑÐ½ÑÐ¼ Ð´Ð¸Ð°Ð±ÐµÑÐ¾Ð¼ Ð¿ÐµÑÐ²Ð¾Ð³Ð¾ ÑÐ¸Ð¿Ð° (2â5 Ð½ÐµÐ´ÐµÐ»Ñ Ð´Ð¸Ð°Ð±ÐµÑÐ°). Ð ÑÐ°Ð±Ð¾ÑÐµ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»ÑÑ Ð¼ÐµÑÐ¾Ð´ Ð¼Ð¸ÐºÑÐ¾ÑÐ»ÐµÐºÑÑÐ¾Ð´Ð½Ð¾Ð¹ Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐµÐ³Ð¸ÑÑÑÐ°ÑÐ¸Ð¸ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð¾Ð² Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ ÐºÐ°ÑÐ´Ð¸Ð¾Ð¼Ð¸Ð¾ÑÐ¸ÑÐ¾Ð². ÐÑÐ» Ð¿ÑÐ¾Ð²ÐµÐ´ÑÐ½ Ð°Ð½Ð°Ð»Ð¸Ð· ÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾ÑÑÐ¸ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ° Ð²Ð¾ Ð²ÑÐµÐ¼ÐµÐ½Ð¸. Ð¢Ð°ÐºÐ¶Ðµ Ð±ÑÐ»Ð¾ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ðµ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸Ðº ÑÐ¿Ð¾Ð½ÑÐ°Ð½Ð½ÑÑ Ð¸ Ð²ÑÐ·Ð²Ð°Ð½Ð½ÑÑ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð¾Ð² Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ, ÑÐµÐ³Ð¸ÑÑÑÐ¸ÑÑÐµÐ¼ÑÑ Ð² ÑÐ°Ð·Ð½ÑÑ ÑÐ°Ð¹Ð¾Ð½Ð°Ñ Ð»ÐµÐ²Ð¾Ð³Ð¾ Ð¶ÐµÐ»ÑÐ´Ð¾ÑÐºÐ° Ð¸ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÑÐ½ ÑÑÑÐµÐºÑ ÑÐ°ÑÐ°ÑÐ½Ð¾Ð³Ð¾ Ð´Ð¸Ð°Ð±ÐµÑÐ° Ð¿ÐµÑÐ²Ð¾Ð³Ð¾ ÑÐ¸Ð¿Ð° Ð½Ð° ÑÐ»ÐµÐºÑÑÐ¾Ð³ÐµÐ½ÐµÐ· ÑÑÐ±ÑÐ¿Ð¸ÐºÐ°ÑÐ´Ð¸Ð°Ð»ÑÐ½ÑÑ ÐºÐ°ÑÐ´Ð¸Ð¾Ð¼Ð¸Ð¾ÑÐ¸ÑÐ¾Ð². ÐÐ¾ Ð¸ÑÐ¾Ð³Ð°Ð¼ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ñ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ. ÐÑÐµÐ¿Ð°ÑÐ°Ñ Ð¸Ð·Ð¾Ð»Ð¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ ÑÐµÑÐ´ÑÐ° ÑÐ¾ÑÐ¾ÑÐ¾ Ð¿ÐµÑÐµÐ¶Ð¸Ð²Ð°ÐµÑ Ð¿ÐµÑÑÑÐ·Ð¸Ñ Ð¿Ð¾ Ð¼ÐµÑÐ¾Ð´Ñ ÐÐ°Ð½Ð³ÐµÐ½Ð´Ð¾ÑÑÐ° Ð¸ ÑÑÐ°Ð±Ð¸Ð»ÐµÐ½ Ð² ÑÐµÑÐµÐ½Ð¸Ðµ Ð¿Ð¾ ÐºÑÐ°Ð¹Ð½ÐµÐ¹ Ð¼ÐµÑÐµ 40 Ð¼Ð¸Ð½ÑÑ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°. Ð¡Ð¿Ð¾Ð½ÑÐ°Ð½Ð½ÑÐµ Ð¸ Ð²ÑÐ·Ð²Ð°Ð½Ð½ÑÐµ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ñ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ ÑÑÐ±ÑÐ¿Ð¸ÐºÐ°ÑÐ´Ð¸Ð°Ð»ÑÐ½ÑÑ Ð¼Ð¸Ð¾ÑÐ¸ÑÐ¾Ð² ÑÐ°Ð·Ð½ÑÑ ÑÐ°Ð¹Ð¾Ð½Ð¾Ð² Ð»ÐµÐ²Ð¾Ð³Ð¾ Ð¶ÐµÐ»ÑÐ´Ð¾ÑÐºÐ° Ð¸Ð·Ð¾Ð»Ð¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ ÑÐµÑÐ´ÑÐ° ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½ÑÑ ÐºÑÑÑ Ð½Ðµ Ð¾ÑÐ»Ð¸ÑÐ°ÑÑÑÑ Ð´ÑÑÐ³ Ð¾Ñ Ð´ÑÑÐ³Ð° Ð¿Ð¾ ÑÐ²Ð¾Ð¸Ð¼ Ð²ÑÐµÐ¼ÐµÐ½Ð½ÑÐ¼ Ð¸ Ð°Ð¼Ð¿Ð»Ð¸ÑÑÐ´Ð½ÑÐ¼ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ°Ð¼. Ð­ÑÑÐµÐºÑ ÐºÑÐ°ÑÐºÐ¾ÑÑÐ¾ÑÐ½Ð¾Ð³Ð¾ ÑÐ°ÑÐ°ÑÐ½Ð¾Ð³Ð¾ Ð´Ð¸Ð°Ð±ÐµÑÐ° Ð¿ÑÐ¾ÑÐ²Ð»ÑÐµÑÑÑ Ð² Ð·Ð½Ð°ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ð¼ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ð¸ ÑÐºÐ¾ÑÐ¾ÑÑÐ¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ Ð²Ð´Ð¾Ð»Ñ Ð²Ð¾Ð»Ð¾ÐºÐ¾Ð½ ÑÐ¸Ð½ÑÐ¸ÑÐ¸Ñ ÑÑÐ±ÑÐ¿Ð¸ÐºÐ°ÑÐ´Ð¸Ð°Ð»ÑÐ½ÑÑ ÐºÐ°ÑÐ´Ð¸Ð¾Ð¼Ð¸Ð¾ÑÐ¸ÑÐ¾Ð² (Ð½Ð°ÑÐ¸Ð½Ð°Ñ ÑÐ¾ 2-Ð½ÐµÐ´ÐµÐ»Ð¸ Ð´Ð¸Ð°Ð±ÐµÑÐ°) Ð¸ ÑÐ¼ÐµÐ½ÑÑÐµÐ½Ð¸Ð¸ Ð°Ð¼Ð¿Ð»Ð¸ÑÑÐ´Ñ Ð²ÑÐ·Ð²Ð°Ð½Ð½ÑÑ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð¾Ð² Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ ÑÑÐ¸Ñ ÐºÐ»ÐµÑÐ¾Ðº (4â5 Ð½ÐµÐ´ÐµÐ»Ñ Ð´Ð¸Ð°Ð±ÐµÑÐ°).
description: This work is devoted to the study of the generation and conduction of action potentials in subepicardial cardiomyocytes of the left ventricle of the isolated heart of control rats and rats with streptozotocin-induced type 1 diabetes mellitus (2â5 weeks of diabetes). Intracellular recording of action potentials of cardiomyocytes was used in the work. An analysis of the stability of the heart preparation over time was carried out. We also compared the characteristics of spontaneous and evoked action potentials recorded in different regions of the left ventricle and determined the effect of type 1 diabetes mellitus on the electrogenesis of subepicardial cardiomyocytes. Based on the studies conducted, the following results were obtained: The isolated and perfused heart preparation is stable for at least 40 minutes of the electrophysiological experiment. Spontaneous and evoked action potentials of subepicardial myocytes from different regions of the left ventricle of the isolated heart of control rats do not differ from each other in their temporal and amplitude characteristics. The effect of short-term diabetes mellitus is manifested in a significant decrease in the conduction velocity along the syncytium fibers of subepicardial cardiomyocytes (starting from the 2nd week of diabetes) and a decrease in the amplitude of the evoked action potentials of these cells (4â5 weeks of diabetes).