title: Ð¡ÑÐ°Ð²Ð½Ð¸ÑÐµÐ»ÑÐ½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð· Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð´ÐµÐºÐ¾Ð½Ð²Ð¾Ð»ÑÑÐ¸Ð¸ Ð´Ð°Ð½Ð½ÑÑ Ð±ÐµÐ»ÐºÐ¾Ð²Ð¾Ð¹ Ð¼Ð°ÑÑ-ÑÐ¿ÐµÐºÑÑÐ¾Ð¼ÐµÑÑÐ¸Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Comparative analysis of methods for deconvolution of protein mass-spectrometry data
Creators: name: ÐÐµÑÑÐ²ÑÐºÐ¸Ð¹, ÐÑÑÐµÐ¼
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: Ð¼Ð°ÑÑ-ÑÐ¿ÐµÐºÑÑÐ¾Ð¼ÐµÑÑÐ¸Ñ
Ð´ÐµÐºÐ¾Ð½Ð²Ð¾Ð»ÑÑÐ¸Ñ
Ms-Deconv
TopFD
HardklÃ¶r
Ð¿ÑÐ¾ÑÐµÐ¾ÑÐ¾ÑÐ¼Ð°
Ð¸Ð·Ð¾ÑÐ¾Ð¿Ð½ÑÐ¹ ÐºÐ»Ð°ÑÑÐµÑ
mass spectrometry
deconvolution
proteoform
isotopomer envelope

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° ÑÑÐ°Ð²Ð½Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð¼Ñ Ð°Ð½Ð°Ð»Ð¸Ð·Ñ ÑÑÐµÑ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð´ÐµÐºÐ¾Ð½Ð²Ð¾Ð»ÑÑÐ¸Ð¸ Ð¼Ð°ÑÑ-ÑÐ¿ÐµÐºÑÑÐ¾Ð² Â«ÑÐ²ÐµÑÑÑ-Ð²Ð½Ð¸Ð·Â». Ð ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð¾Ð±ÑÐµÐºÑÐ¾Ð² Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð±ÑÐ»Ð¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ Ð¼ÐµÑÐ¾Ð´Ñ, ÑÐµÐ°Ð»Ð¸Ð·Ð¾Ð²Ð°Ð½Ð½ÑÐµ Ð² ÑÐ°ÐºÐ¸Ñ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ð½ÑÑ Ð¸Ð½ÑÑÑÑÐ¼ÐµÐ½ÑÐ°Ñ ÐºÐ°Ðº TopFD, Ms-Deconv, HardklÃ¶r. Ð Ð¿ÐµÑÐ²ÑÑ Ð¾ÑÐµÑÐµÐ´Ñ Ð±ÑÐ»Ð¸ ÑÐ°ÑÑÐ¼Ð¾ÑÑÐµÐ½Ñ ÑÐµÐ¾ÑÐµÑÐ¸ÑÐµÑÐºÐ¸Ðµ Ð°ÑÐ¿ÐµÐºÑÑ ÑÐºÐ°Ð·Ð°Ð½Ð½ÑÑ Ð¼ÐµÑÐ¾Ð´Ð¾Ð², Ð° Ð·Ð°ÑÐµÐ¼ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¾ Ð¸Ñ ÑÐµÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð½Ð° Ð½Ð°Ð±Ð¾ÑÐµ Ð¼Ð°ÑÑ-ÑÐ¿ÐµÐºÑÑÐ¾Ð² Â«ÑÐ²ÐµÑÑÑ-Ð²Ð½Ð¸Ð·Â» ÑÐµÐ»Ð¾Ð²ÐµÑÐµÑÐºÐ¾Ð³Ð¾ Ð³Ð¸ÑÑÐ¾Ð½Ð° H4. ÐÐ°Ð»ÐµÐµ Ð¿Ð¾ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÐ¼ Ð´ÐµÐºÐ¾Ð½Ð²Ð¾Ð»ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¼ Ð¼Ð°ÑÑ-ÑÐ¿ÐµÐºÑÑÐ°Ð¼ Ð±ÑÐ»Ð° Ð¿ÑÐ¾Ð¸Ð·Ð²ÐµÐ´ÐµÐ½Ð° Ð¸Ð´ÐµÐ½ÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ñ Ð±ÐµÐ»ÐºÐ¾Ð² Ð¸ Ð¿ÑÐ¾ÑÐµÐ¾ÑÐ¾ÑÐ¼ Ð¿Ð¾ Ð±Ð°Ð·Ðµ Ð´Ð°Ð½Ð½ÑÑ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ð½Ð¾Ð³Ð¾ Ð¸Ð½ÑÑÑÑÐ¼ÐµÐ½ÑÐ° TopPIC. ÐÑÐ¾Ð³Ð¾Ð²ÑÐ¹ ÑÑÐ°Ð²Ð½Ð¸ÑÐµÐ»ÑÐ½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð· Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»ÑÑ Ð¿Ð¾ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ°Ð¼ Ð¿ÑÐµÐ´ÑÐ´ÑÑÐµÐ³Ð¾ ÑÑÐ°Ð¿Ð° Ð¸Ð´ÐµÐ½ÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸. Ð¢ÐµÐºÑÑÐ¸Ðµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð´ÐµÐºÐ¾Ð½Ð²Ð¾Ð»ÑÑÐ¸Ð¸ Ð´Ð°Ð½Ð½ÑÑ Ð±ÐµÐ»ÐºÐ¾Ð²Ð¾Ð¹ Ð¼Ð°ÑÑ-ÑÐ¿ÐµÐºÑÑÐ¾Ð¼ÐµÑÑÐ¸Ð¸ Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÑÑ ÑÐ´ÐµÐ»Ð°ÑÑ Ð¿ÑÐµÐ´Ð²Ð°ÑÐ¸ÑÐµÐ»ÑÐ½ÑÐ¹ Ð²ÑÐ²Ð¾Ð´, ÑÑÐ¾ Ð´Ð»Ñ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ Ð¿Ð¾Ð»Ð½Ð¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð¼Ð°ÑÑ-ÑÐ¿ÐµÐºÑÑÐ¾Ð² Â«ÑÐ²ÐµÑÑÑ-Ð²Ð½Ð¸Ð·Â» Ð¾ÑÐ´ÐµÐ»ÑÐ½ÑÑ Ð±ÐµÐ»ÐºÐ¾Ð² ÑÐ»ÐµÐ´ÑÐµÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°ÑÑ ÑÑÐ°Ð·Ñ Ð´Ð²Ð° Ð¸Ð½ÑÑÑÑÐ¼ÐµÐ½ÑÐ° Ð´ÐµÐºÐ¾Ð½Ð²Ð¾Ð»ÑÑÐ¸Ð¸ Ms-Deconv Ð¸ HardklÃ¶r.
description: This work is devoted to a comparative analysis of three top-down mass spectral deconvolution methods. The methods used in such software tools as TopFD, Ms-Deconv, HardklÃ¶r were subject to analysis. First, the theoretical aspects of these methods were considered, and then they were tested on a set of top-down mass spectra of human histone H4. Further, based on the obtained deconvoluted mass spectra, proteins and proteoforms were identified by the database search with the TopPIC software tool. The final comparative analysis was carried out based on the results of the previous stage of identification. The current results of the analysis of methods for deconvolution of protein mass spectrometry data allow us to make a preliminary conclusion that for the most complete analysis of the "top-down" mass spectra of individual proteins, two deconvolution tools Ms-Deconv and HardklÃ¶r should be used at once.