title: ÐÐ±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¸Ðµ Ð¸ Ð¿ÐµÑÐ²Ð¸ÑÐ½Ð°Ñ Ñ Ð°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ° Ð³ÑÑÐ¿Ð¿Ñ Ð²Ð½ÐµÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð¼ÐµÑÐ°Ð»Ð»Ð¾Ð¿ÐµÐ¿ÑÐ¸Ð´Ð°Ð· Ð¼Ð¾Ð·Ð³Ð° Ð¿ÑÐ¸Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Detection and primary characterization of a group of extracellular metallopeptidases of the brain of birds
Creators: name: Ð¡Ð¸ÑÐ´Ð¸ÐºÐ¾Ð²Ð°, ÐÑÐµÐ½Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: Ð¼ÐµÑÐ°Ð»Ð»Ð¾Ð¿ÐµÐ¿ÑÐ¸Ð´Ð°Ð·Ñ
Ð°ÐºÑÐ¾Ð½Ð½ÑÐµ Ð¾ÐºÐ¾Ð½ÑÐ°Ð½Ð¸Ñ
ÑÐ¸Ð½Ð°Ð¿ÑÐ¾ÑÐ¾Ð¼Ñ
ÐºÑÑÐ¸ÑÐ°
ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ðµ Ð¿ÑÐ¸Ñ Ð¸ Ð¼Ð»ÐµÐºÐ¾Ð¿Ð¸ÑÐ°ÑÑÐ¸Ñ
Ð¼etallopeptidase
axonal ends
synaptosomes
chicken
comprasion of birds and mammalians

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¿ÐµÑÐ²Ð¸ÑÐ½Ð¾Ð¹ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐµ Ð²Ð½ÐµÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð¼ÐµÑÐ°Ð»Ð»Ð¾Ð¿ÐµÐ¿ÑÐ¸Ð´Ð°Ð·, Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½ÑÑ Ñ Ð¿Ð¾Ð²ÐµÑÑÐ½Ð¾ÑÑÐ¸ ÑÐ¸Ð½Ð°Ð¿ÑÐ¾ÑÐ¾Ð¼ (Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½ÑÑ Ð°ÐºÑÐ¾Ð½Ð°Ð»ÑÐ½ÑÑ Ð¾ÐºÐ¾Ð½ÑÐ°Ð½Ð¸Ð¹) Ð¼Ð¾Ð·Ð³Ð° Ð¿ÑÐ¸Ñ, Ð° ÑÐ°ÐºÐ¶Ðµ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ ÑÑÐ¸Ñ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð°Ð· Ñ ÑÐµÑÐ¼ÐµÐ½ÑÐ°Ð¼Ð¸ NEMP, Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½ÑÑ Ñ Ð¿Ð¾Ð²ÐµÑÑÐ½Ð¾ÑÑÐ¸ ÑÐ¸Ð½Ð°Ð¿ÑÐ¾ÑÐ¾Ð¼ Ð¸Ð· Ð¼Ð¾Ð·Ð³Ð° Ð¼Ð»ÐµÐºÐ¾Ð¿Ð¸ÑÐ°ÑÑÐ¸Ñ.
Ð­ÐºÑÑÑÐ°ÐºÑ Ð¼ÐµÑÐ°Ð»Ð»Ð¾Ð¿ÐµÐ¿ÑÐ¸Ð´Ð°Ð· Ð¿Ð¾Ð»ÑÑÐ°Ð»Ð¸ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÐºÑÐ°ÑÐºÐ¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð¹ Ð¸Ð½ÐºÑÐ±Ð°ÑÐ¸Ð¸ ÑÐ¸Ð½Ð°Ð¿ÑÐ¾ÑÐ¾Ð¼, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð¿Ð¾ÑÐ»Ðµ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÐµÐ½ÑÑÐ¸ÑÑÐ³Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¸ Ð¾ÑÐ¸ÑÑÐºÐ¸ Ð² Ð³ÑÐ°Ð´Ð¸ÐµÐ½ÑÐµ ÑÐ°ÑÐ°ÑÐ¾Ð·Ñ, Ñ Ð½ÐµÐ¸Ð¾Ð½Ð½ÑÐ¼ Ð´ÐµÑÐµÑÐ³ÐµÐ½ÑÐ¾Ð¼ TritonX-100. Ð¤ÐµÑÐ¼ÐµÐ½ÑÑ Ð·Ð°ÑÐµÐ¼ ÑÐ°Ð·Ð´ÐµÐ»ÑÐ»Ð¸ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÐ¼Ð¸ Ð¼ÐµÑÐ¾Ð´Ð°Ð¼Ð¸ (ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÐ· Ð² Ð½ÐµÐ´ÐµÐ½Ð°ÑÑÑÐ¸ÑÑÑÑÐ¸Ñ ÑÑÐ»Ð¾Ð²Ð¸ÑÑ, Ð¸Ð¾Ð½Ð¾Ð¾Ð±Ð¼ÐµÐ½Ð½Ð°Ñ ÑÑÐ¾Ð¼Ð°ÑÐ¾Ð³ÑÐ°ÑÐ¸Ñ Ð¸ ÑÑÐ¾Ð¼Ð°ÑÐ¾Ð³ÑÐ°ÑÐ¸Ñ Ð½Ð° Ð³Ð¸Ð´ÑÐ¾ÐºÑÐ¸Ð°Ð¿Ð°ÑÐ¸ÑÐµ). 
Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¾ Ð²ÑÑÐ²Ð»ÐµÐ½Ð¾, ÑÑÐ¾ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐµÑÐ¼ÐµÐ½ÑÑ ÑÐ²Ð»ÑÑÑÑÑ Ð¼ÐµÑÐ°Ð»Ð»Ð¾Ð¿ÐµÐ¿ÑÐ¸Ð´Ð°Ð·Ð°Ð¼Ð¸. Ð¢Ð°ÐºÐ¶Ðµ Ð±ÑÐ»Ð¾ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¾, ÑÑÐ¾ Ð´Ð°Ð½Ð½ÑÐµ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð°Ð·Ñ Ð½Ðµ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ñ Ð³Ð¸Ð´ÑÐ¾Ð»Ð¸Ð·Ð¾Ð²Ð°ÑÑ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð½ÑÑ ÑÐ²ÑÐ·Ñ Ð² Ð¼Ð¾Ð»ÐµÐºÑÐ»Ðµ ÐºÐ°ÑÐ½Ð¾Ð·Ð¸Ð½Ð° (Î²Ala-His). Ð­ÑÐ¾ Ð´Ð°Ð»Ð¾ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾ÑÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°ÑÑ Ð½Ð°Ð±Ð¾Ñ ÑÐµÑÑÐ¾Ð²ÑÑ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð² Ñ ÐºÐ°ÑÐ½Ð¾Ð·Ð¸Ð½Ð¾Ð¼ Ð½Ð° ÑÐ°Ð·Ð½ÑÑ ÐºÐ¾Ð½ÑÐ°Ñ Ð¸ Ð¾Ð¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ Ð²Ð¸Ð´ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½ÑÑ ÑÐµÑÐ¼ÐµÐ½ÑÐ¾Ð². Ð¢Ð°ÐºÐ¸Ð¼ Ð¾Ð±ÑÐ°Ð·Ð¾Ð¼, ÑÑÐµÐ´Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ ÑÐµÑÐ¼ÐµÐ½ÑÐ¾Ð² Ð±ÑÐ»Ð¸ Ð²ÑÑÐ²Ð»ÐµÐ½Ñ ÐºÐ°ÑÐ±Ð¾ÐºÑÐ¸Ð¿ÐµÐ¿ÑÐ¸Ð´Ð°Ð·Ð°, Ð°Ð¼Ð¸Ð½Ð¾Ð¿ÐµÐ¿ÑÐ¸Ð´Ð°Ð·Ð°, ÑÐ½Ð´Ð¾Ð¿ÐµÐ¿ÑÐ¸Ð´Ð°Ð·Ð° Ð¸ ÑÑÐ¸Ð¿ÐµÐ¿ÑÐ¸Ð´Ð°Ð·Ð°. Ð Ð¾ÑÐ»Ð¸ÑÐ¸Ðµ Ð¾Ñ NEMP Ð¼Ð»ÐµÐºÐ¾Ð¿Ð¸ÑÐ°ÑÑÐ¸Ñ, Ñ Ð¿ÑÐ¸Ñ ÑÑÐµÐ´Ð¸ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½ÑÑ ÑÐµÑÐ¼ÐµÐ½ÑÐ¾Ð² Ð¾ÑÑÑÑÑÑÐ²ÑÐµÑ Ð´Ð¸Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¸Ð»-Ð¿ÐµÐ¿ÑÐ¸Ð´Ð°Ð·Ð°, Ð½Ð¾ ÐµÑÑÑ ÑÑÐ¸Ð¿ÐµÐ¿ÑÐ¸Ð´Ð°Ð·Ð°. Ð ÑÐ°Ðº Ð¶Ðµ, ÐºÐ°Ðº Ð² ÑÐ»ÑÑÐ°Ðµ Ñ Ð¼Ð»ÐµÐºÐ¾Ð¿Ð¸ÑÐ°ÑÑÐ¸Ð¼Ð¸, Ð½ÐµÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð°Ð· ÑÐ°ÑÑÐµÐ¿Ð»ÑÑÑ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð½ÑÑ ÑÐ²ÑÐ·Ñ Ñ Î²-Ala Ð´Ð°ÐµÑ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾ÑÑÑ ÑÐ¾Ð·Ð´Ð°Ð²Ð°ÑÑ Ð·Ð°ÑÐ¸ÑÐµÐ½Ð½ÑÐµ Ð¾Ñ ÑÑÐ¸Ñ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð°Ð· Ð»ÐµÐºÐ°ÑÑÑÐ²ÐµÐ½Ð½ÑÐµ Ð¿ÐµÐ¿ÑÐ¸Ð´Ñ.
description: The given work is devoted to determining the characteristics of extracellular metallopeptidases isolated from the surface of synaptosomes of birds' brains, as well as comparing these peptidases with NEMPs - enzymes isolated from the mammalâs brain.
Metallopeptidase extracted from the surface of the synaptosome with the de-tergent Triton X-100 obtained after differential centrifugation and ultracentrifuga-tion. The enzymes are then separated by various methods (electrophoresis under non-denaturing conditions, ion exchange and hydroxyapatite chromatography). To determine the activities that are detected using various test peptides.
As a result of the work, those enzymes were identified that are metallopeptidases were defined as aminopeptidase, carboxypeptidase, endopeptidase and tripeptidase, which distinguish them from NEMPs that do not contain enzymes with tripeptidase activity, but contain enzymes with dipeptidyl peptidase activity. Also, obtained enzymes that destroys tri- and dipeptides to individual acids is not able to hydrolyze the peptide bond in carnosine, which suggests the possibility of creating protected peptides (including drugs based on them) that do not degrade in accordance with metallopeptidases.