Additional file 7 of Identification of autophagy-related risk signatures for the prognosis, diagnosis, and targeted therapy in cervical cancer
Creators: name: Meng, Dan
name: Jin, Hua
name: Zhang, Xing
name: Yan, Wenjing
name: Xia, Qianqian
name: Shen, Siyuan
name: Xie, Shuqian
name: Cui, Mengjing
name: Ding, Bo
name: Gu, Yun
name: Wang, Shizhi
Publisher: name: figshare
Year: 2021
Subjects: Medicine
Cell Biology
Pharmacology
Biotechnology
Biological Sciences not elsewhere classified
Mathematical Sciences not elsewhere classified
Cancer

description: Additional file 7: Figure S1. The box plot of 53 DEARGs and barplot of PPI network. a. The expression pattern of DEARGs in CC and normal samples. b. The number of nodes in the top 30 autophagy-related genes of the PPI network. Figure S2. Immunohistochemistry (IHC) results showing protein levels of autophagy-related genes in CC and normal tissues. a. IHC results of ATG4C in CC (staining:Medium; intensity:Moderate; quantity: &gt; 75%; location: Cytoplasmic membranous) and in normal tissue (staining:Low;intensity:Weak; quantity:75%-25%; location:Cytoplasmic membranous).b. IHC results of ATG4D in CC (staining:Not detected; intensity:Negative; quantity:None; location:None) and in normal tissue(staining: Low;intensity:Weak; quantity:75%-25%; location:Cytoplasmic membranous). c. IHC results of CD46 in CC(staining:Medium; intensity:Moderate; quantity: &gt; 75%; location:Cytoplasmic membranous) and in normal tissue(staining:Low; intensity:Weak; quantity: &gt; 75%; location: Cytoplasmic membranous). d. IHC results of HGS in CC(staining:Not detected; intensity:Negative; quantity:None; location:None) and in normal tissue(staining:Not detected; intensity:Negative; quantity:None; location:None). e. IHC results of HSPB8 in CC(staining: Medium; intensity:Moderate; quantity: &gt; 75%; location:Cytoplasmic membranous) and in normal tissue(staining:High; intensity:Strong; quantity: &gt; 75%; location:Cytoplasmic membranous). f. IHC results of KLHL24 in CC(staining:Not detected; intensity:Negative; quantity:None; location:None) and in normal tissue(staining:Not detected; intensity:Negative; quantity:None; location:None). g. IHC results of MTMR14 in CC(staining:Not detected; intensity:Negative; quantity:None; location: None) and in normal tissue(staining:Not detected; intensity:Negative; quantity:None; location: None). h. IHC results of NAMPT in CC(staining:Not detected; intensity:Negative; quantity:None; location:None) and in normal tissue(staining:Not detected; intensity:Negative; quantity:None; location:None). i. IHC results of SERPINA1 in CC(staining:Not detected; intensity:Negative; quantity:None; location:None) and in normal tissue(staining:Not detected; intensity:Negative; quantity:None; location:None). j. IHC results of SUPT20H in CC(staining:Medium; intensity:Moderate; quantity: &gt; 75%; location:Cytoplasmic membranous) and in normal tissue(staining:Medium; intensity:Moderate; quantity: &gt; 75%; location:Cytoplasmic membranous). k. IHC results of TP73 in CC(staining:Not detected; intensity:Negative; quantity:None; location: None) and in normal tissue(staining:Medium;intensity:Strong; quantity: &lt; 25%;location:Nuclear). l. IHC results of VAMP7 in CC(staining:Medium; intensity:Moderate; quantity: &gt; 75%; location: Cytoplasmic membranous) and in normal tissue(staining:Medium; intensity:Moderate; quantity: 75%-25%; location:Cytoplasmic membranous). Figure S3. Kaplan–Meier survival curves for CC based on the prognostic risk genes. a-l shows the results of ATG4C, ATG4D, CD46, HGS, KLHL24, MTMR14, NAMPT, TP73, VAMP7, HSPB8, SERPINA1 and SUPT20H respectively. Figure S4. Calibration curve of nomogram diagram and exploration of risk signature in the testing set. a-c. Calibration plots for 1-year, 3-year and 5-year survival for the nomogram of OS model. d-f. Calibration plots for 1-year, 3-year and 5-year survival for the nomogram of RFS model. g. K-M curve of the high- and low-risk UCEC patients in the testing group for OS model. h. The 3-year, 5-year and 7-year ROC curves in the testing group of UCEC patients for OS. i. K-M curve of the high- and low-risk HNSCC patients in the testing group for the RFS model. j. The 1-year, 3-year and 5-year ROC curves in the testing group of HNSCC patients for RFS. Figure S5. AUC of risk signature without diagnostic value. a-f. AUC of risk signature without diagnostic value (HGS, KLHL24, NAMPT, SERPINA1, TP73, NAMP7). Figure S6. Single gene function enrichment analysis and target sensitivity detection. a-k. Single gene function enrichment analysis of 12 prognostic risk signatures.