MOESM1 of Laccase-derived lignin compounds boost cellulose oxidative enzymes AA9
Creators: name: Brenelli, Lívia
name: Squina, Fabio
name: Felby, Claus
name: Cannella, David
Publisher: name: figshare
Year: 2018
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Pharmacology
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: FOS: Chemical sciences
subject: Ecology
subject: Immunology
subject: FOS: Clinical medicine
subject: FOS: Clinical medicine
subject: Cancer
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: FOS: Health sciences
subject: Plant Biology

description: Additional file 1: Table S1. Enzymatic hydrolysis of Avicel supplemented with supernatants rich in low-molecular-weight lignin-derived compounds. Quantification of gluconic acid in g/Kg (first column), relative yield of cellulose oxidation calculated as % of the amount of gluconic acid in [g/kg]/ glucose [g/kg]. Figure S1. RP-HPLC analysis of the supernatants obtained for the pre-treated sugarcane bagasse incubated with buffer and ABTS (black line), LMS_MtL (blue line), or LMS_TvL (pink line): chromatograms relative to 308 nm wavelength. The new peaks that appeared after LMS treatment are circled by red circles. Table S2. List of compounds detected by RP-HPLC analysis from the supernatant obtained for the pre-treated sugarcane bagasse incubated with buffer and ABTS (Control), LMS_TvL, and LMS_MtL. The retention time, wavelength of maximum absorption and peak area, and peak height for each compound are reported. The height of each peak is in proportion to the amount of the component present in the sample mixture. Figure S2. RP-HPLC analysis of the supernatants obtained for the pre-treated wheat straw incubated with buffer and ABTS (black line), LMS_MtL (blue line), or LMS_TvL (pink line): chromatograms relative to 308 nm wavelength. The new peaks that appeared after LMS treatment are circled by red circles. Table S3. List of compounds detected by RP-HPLC analysis from the supernatant obtained for the pre-treated wheat straw incubated with buffer and ABTS (Control), LMS_TvL, and LMS_MtL. The retention time, wavelength of maximum absorption and peak area, and peak height for each compound are reported. The height of each peak is in proportion to the amount of the component present in the sample mixture. Table S4. Relative absorbance of bands in the infrared spectrum of different groups in the control experiment contained Avicel and ABTS, Avicel treated with LMS based on MtL laccase (Avicel + LMS_MtL) and LMS based on TvL laccase (Avicel + LMS_TvL). The data shown are from the normalized spectra. Figure S3. Enzymatic hydrolysis (A) and Fourier transform-infrared spectra (B) of Avicel treated with LMS based on MtL laccase (Avicel + LMS_MtL in A; red line in B) and LMS based on TvL laccase (Avicel + LMS_TvL in A, blue line in B), the control experiment contained Avicel and ABTS, lacking of laccases (Avicel in A; black line in B). (*) The mean difference is statistically significant at the 0.05 level by the Tukey test. The arrows indicate the main changes in the spectra after LMS treatment. Figure S4. Cellulose conversion % of hydrothermal sugarcane bagasse and wheat straw treated with laccase mediator system, followed by laccase inactivation, washing procedure and hydrolyzed using Cellic® CTec2 for 72 h. Error bars represent the standard errors of the means of triplicate experiments. Legend: CT2—Cellic® CTec2, SCB—pre-treated sugarcane bagasse, WS—pre-treated wheat straw, LMS_MtL—MtL laccase mediator system treatment, LMS_TvL—TvL laccase mediator system treatment, ABTS—only mediator treatment. Figure S5. Fourier transform-infrared (FT-IR) spectra of pre-treated sugarcane bagasse (A) and wheat straw (B) after ABTS only (black), LMS_MtL (red), and LMS_TvL (blue) treatment. Table S5. Relative absorbance of bands in the infrared spectrum of different groups in the untreated and LMS-pre-treated sugarcane bagasse and wheat straw samples. Legend: SCB: sugarcane bagasse, WS: wheat straw, LMS_MtL—MtL laccase mediator system treatment, LMS_TvL—TvL laccase mediator system treatment.*From the normalized spectra.