cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
openFrame: A modular, sustainable, open microscopy platform with single-shot, dual-axis optical autofocus module providing high precision and long range of operation.
Creators: name: Lightley, J
name: Kumar, S
name: Lim, MQ
name: Garcia, E
name: Görlitz, F
name: Alexandrov, Y
name: Parrado, T
name: Hollick, C
name: Steele, E
name: Roßmann, K
name: Graham, J
name: Broichhagen, J
name: McNeish, IA
name: Roufosse, CA
name: Neil, MAA
name: Dunsby, C
name: French, PMW
Publisher: name: The Francis Crick Institute
Year: 2023
Subjects: classificationCode: Imaging
subject: Imaging

description: "openFrame" is a modular, low-cost, open-hardware microscopy platform that can be configured or adapted to most light microscopy techniques and is easily upgradeable or expandable to multiple modalities. The ability to freely mix and interchange both open-source and proprietary hardware components or software enables low-cost, yet research-grade instruments to be assembled and maintained. It also enables rapid prototyping of advanced or novel microscope systems. For long-term time-lapse image data acquisition, slide-scanning or high content analysis, we have developed a novel optical autofocus incorporating orthogonal cylindrical optics to provide robust single-shot closed-loop focus lock, which we have demonstrated to accommodate defocus up to ±37 μm with <200 nm accuracy, and a 2-step autofocus mode which we have shown can operate with defocus up to ±68 μm. We have used this to implement automated single molecule localization microscopy (SMLM) in a relatively low cost openFrame-based instrument using multimode diode lasers for excitation and cooled CMOS cameras. This article is protected by copyright. All rights reserved.
Matrix factorisations for rational boundary conditions by defect fusion
Creators: name: Behr, Nicolas
name: Fredenhagen, Stefan
Publisher: name: arXiv
Year: 2014
Subjects: subject: High Energy Physics - Theory (hep-th)
subject: Mathematical Physics (math-ph)
subject: FOS: Physical sciences

description: A large class of two-dimensional $\mathcal{N}=(2,2)$ superconformal field theories can be understood as IR fixed-points of Landau-Ginzburg models. In particular, there are rational conformal field theories that also have a Landau-Ginzburg description. To understand better the relation between the structures in the rational conformal field theory and in the Landau-Ginzburg theory, we investigate how rational B-type boundary conditions are realised as matrix factorisations in the $SU(3)/U(2)$ Grassmannian Kazama-Suzuki model. As a tool to generate the matrix factorisations we make use of a particular interface between the Kazama-Suzuki model and products of minimal models, whose fusion can be realised as a simple functor on ring modules. This allows us to formulate a proposal for all matrix factorisations corresponding to rational boundary conditions in the $SU(3)/U(2)$ model.
description: 33+23 pages, 3 figures
Integrated evaluation of energy system in Sri Lanka: a multidimensional sustainability perspective
Creators: name: Konara, Konara Mudiyanselage Gayani Kaushalya
name: Tokai, Akihiro
Publisher: name: Taylor & Francis
Year: 2022
Subjects: subject: Space Science
subject: Medicine
subject: Biotechnology
subject: Ecology
subject: FOS: Biological sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Science Policy

description: Increase in energy demand in Sri Lanka caused by rapid economic growth needs to be tackled in a sustainable way. Thus, this research intends to retrace the path of energy development during the last two decades from a multidimensional sustainability perspective which in return will help predicting its future behaviour. Integrated approach consists of a system dynamic model developed based on economic and socio-demographic factors, an indictor-based framework with stakeholder participation and a scenario analysis. Results show post-conflict economic development has taken a toll on the overall sustainability of the energy system which has become stagnant since 2010. Alternative scenarios suggest supply-side energy reforms have the highest impact on lessening the environmental burden of the energy system. Findings can provide insights and a structure guidance for the decision-makers to set the energy sector of Sri Lanka on a sustainable development path that is imperative in the long run.
Intravenous cyclosporine activates afferent and efferent renal nerves and causes sodium retention in innervated kidneys in rats.
Creators: name: Falk, R. J.
name: Powell, S. L.
name: Moss, N. G.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1985

description: The effect of acute intravenous infusion of cyclosporine (10 mg/kg) on efferent renal and genitofemoral nerve activity and afferent renal nerve activity was studied in anesthetized rats. All animals were studied after unilateral renal denervation and extracellular fluid volume expansion. Activity of both efferent sympathetic nerves was increased significantly by cyclosporine infusion (renal, 69%; genitofemoral, 60%). Afferent renal nerve activity was increased 82% after cyclosporine (P
Technologische Beschreibung und physiologische Bewertung eines hochaufgelösten Laserscanner-Scheinwerfersystems
Creators: name: Ansorg, Philipp
Publisher: name: KIT Scientific Publishing
Year: 2023
Subjects: Scheinwerfer
Laser-Scanner
Homogenität
Fahrzeug-Lichtfunktionen
Headlamp
laser scanning
homogeneity
automotive light functions

description: Beim Ansatz eines Laserscannerscheinwerfers zur Realisierung von adaptiven Lichtfunktionen werden sechs Laserdioden auf einen Mikrospiegel gelenkt, welcher durch seine schwingende Bewegung eine ausgedehnte Lichtverteilung auf einem Weißlichtkonverter erzeugt ehe diese auf die Fahrbahn projiziert wird. Diese Arbeit beschreibt diesen Ansatz und zeigt Methoden zur Homogenitätsbewertung von Scheinwerferlichtverteilungen.
Morphogenetic rearrangement of injected collagen in developing chicken limb buds.
Creators: name: Stopak, D.
name: Wessells, N. K.
name: Harris, A. K.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1985

description: A fundamental question concerning the development of the extracellular matrix is what factors control the arrangement of collagen fibrils within a tissue and at the same time allow for the great diversity of geometric forms exhibited by collagen. In this report, we test the possibility that physical forces within the embryo serve to organize collagen fibers into regular patterns. In particular, we test the prediction that patterns of stress having this morphogenetic function are generated by cell traction, the contractile force exerted by cells to propel themselves. To study the effects of these mechanical forces on the extracellular matrix, type I collagen was fluorescently labeled and injected into developing chicken wing buds. When the injected limbs were allowed to develop and then examined histologically, the exogenous collagen was found incorporated within normal connective tissues of the wing. The labeled collagen became arranged according to its site of injection, forming parts of tendons, perichondria, cartilages, perineuria, and blood vessels. Since the injected collagen formed a gel within minutes of its injection, the subsequent incorporation of this performed collagen within organized structures cannot be explained in terms of molecular self-assembly or other mechanisms occurring during collagen deposition. These results demonstrate that, within developing tissues, patterns of forces exist that are capable of physically rearranging collagen and determining its long-range order.
Reproducibility for Urban Mobility Analysis
Creators: name: Nüst, Daniel
Publisher: name: Zenodo
Year: 2022
Subjects: reproducible research
urban mobility
transport

description: Slides for a session on open reproducible research at the Dagstuhl Seminar 22162 - Urban Mobility Analytics. The session covers theoretical and practical basics of reproducibility, gives advise on working reproducibly, shows how reproducibility can be integrated into scientific publication and reviewing, presents literature for further studies, and outlines specifics of reproducibility in transport research and urban mobility research.
Anisotropy in Fracking: A Percolation Model for Observed Microseismicity
Creators: name: Norris, J. Quinn
name: Turcotte, Donald L.
name: Rundle, John B.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Geophysics (physics.geo-ph)
subject: Computational Physics (physics.comp-ph)
subject: FOS: Physical sciences

description: Hydraulic fracturing (fracking) using high pressures and a low viscosity fluid allow the extraction of large quantiles of oil and gas from very low permeability shale formations. The initial production of oil and gas at depth leads to high pressures and an extensive distribution of natural fractures which reduce the pressures. With time these fractures heal, sealing the remaining oil and gas in place. High volume fracking opens the healed fractures allowing the oil and gas to flow the horizontal productions wells. We model the injection process using invasion percolation. We utilize a 2D square lattice of bonds to model the sealed natural fractures. The bonds are assigned random strengths and the fluid, injected at a point, opens the weakest bond adjacent to the growing cluster of opened bonds. Our model exhibits burst dynamics in which the clusters extends rapidly into regions with weak bonds. We associate these bursts with the microseismic activity generated by fracking injections. A principal object of this paper is to study the role of anisotropic stress distributions. Bonds in the $y$-direction are assigned higher random strengths than bonds in the $x$-direction. We illustrate the spatial distribution of clusters and the spatial distribution of bursts (small earthquakes) for several degrees of anisotropy. The results are compared with observed distributions of microseismicity in a fracking injection. Both our bursts and the observed microseismicity satisfy Gutenberg-Richter frequency-size statistics.
description: 14 pages, 10 figures
Public health advocacy strategies to influence policy agendas: lessons from a narrative review of success in trade policy
Creators: name: Townsend, Belinda
name: Tenni, Brigitte
name: Goldman, Sharni
name: Gleeson, Deborah
Publisher: name: La Trobe
Year: 2023
Subjects: classificationCode: 4407
subject: Policy and administration
classificationCode: 4203
subject: Health services and systems
classificationCode: 4206
subject: Public health

description: Background: Despite accumulating evidence of the implications of trade policy for public health, trade and health sectors continue to operate largely in silos. Numerous barriers to advancing health have been identified, including the dominance of a neoliberal paradigm, powerful private sector interests, and constraints associated with policymaking processes. Scholars and policy actors have recommended improved governance practices for trade policy, including: greater transparency and accountability; intersectoral collaboration; the use of health impact assessments; South-South networking; and mechanisms for civil society participation. These policy prescriptions have been generated from specific cases, such as the World Trade Organization’s Doha Declaration on TRIPS and Public Health or specific instances of trade-related policymaking at the national level. There has not yet been a comprehensive analysis of what enables the elevation of health goals on trade policy agendas. This narrative review seeks to address this gap by collating and analysing known studies across different levels of policymaking and different health issues. Results: Sixty-five studies met the inclusion criteria and were included in the review. Health issues that received attention on trade policy agendas included: access to medicines, food nutrition and food security, tobacco control, non-communicable diseases, access to knowledge, and asbestos harm. This has occurred in instances of domestic and regional policymaking, and in bilateral, regional and global trade negotiations, as well as in trade disputes and challenges. We identified four enabling conditions for elevation of health in trade-related policymaking: favourable media attention; leadership by trade and health ministers; public support; and political party support. We identified six strategies successfully used by advocates to influence these conditions: using and translating multiple forms of evidence, acting in coalitions, strategic framing, leveraging exogenous factors, legal strategy, and shifting forums. Conclusion: The analysis demonstrates that while technical evidence is important, political strategy is necessary for elevating health on trade agendas. The analysis provides lessons that can be explored in the wider commercial determinants of health where economic and health interests often collide.
Purified human factor VIII procoagulant protein: comparative hemostatic response after infusions into hemophilic and von Willebrand disease dogs.
Creators: name: Garris, J. B.
name: Sandberg, H.
name: Brinkhous, K. M.
name: Read, M. S.
name: Griggs, T.
name: Mattsson, C.
name: Palm, M.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1985

description: The procoagulant protein F.VIII:C is noncovalently bound to von Willebrand factor (vWF) to give the factor VIII macromolecular complex. New highly purified preparations of isolated human F.VIII:C, devoid of vWF and about 500,000-fold purified, were administered to hemophilia A and von Willebrand disease (vWD) dogs to determine their hemostatic effectiveness and survival in the circulation. Two preparations of F.VIII:C were used: peak 1, with active components of Mr 185,000-280,000, and peak 2, with a single component of Mr 170,000. In hemophilic dogs, with no plasma F.VIII:C but normal vWF, both preparations immediately elevated plasma F.VIII:C to expected levels, promptly stopped induced and spontaneous hemorrhages, and gave sustained plasma levels of F.VIII:C. The isolated F.VIII:C immediately complexed with endogenous vWF in hemophilic plasma and was eliminated exponentially, with a half-life (t1/2) of about 9 hr. Survival of peak 2 F.VIII:C was longer than that of peak 1 material. In contrast, F.VIII:C complexed to vWF in a therapeutic concentrate administered to hemophilic dogs was eliminated biexponentially with first-phase t1/2 of 3.2 hr and second-phase t1/2 of 9 hr. In vWD dogs with no vWF and reduced F.VIII:C levels, the isolated F.VIII:C produced supernormal levels of F.VIII:C without effect on induced bleeding. It was rapidly eliminated from plasma with a t1/2 of about 1 hr, as was the complexed F.VIII:C in the concentrate. These data indicate that isolated F.VIII:C promptly complexes with vWF and in this form is highly effective in controlling hemophilic hemorrhages with good survival in plasma. Without endogenous vWF with which to complex, the F.VIII:C is promptly eliminated.
Rat Sertoli cells acquire a beta-adrenergic response during primary culture.
Creators: name: Perkins, J. P.
name: White, M. G.
name: Kierszenbaum, A. L.
name: Tres, L. L.
name: Spruill, W. A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1985

description: Two-dimensional polyacrylamide gel electrophoresis and the radioligand (-)-[125I]iodopindolol (125I-Pin) have been used to study isoproterenol-dependent protein phosphorylation and beta-adrenergic receptor availability, respectively, in cultured Sertoli cells and freshly isolated seminiferous tubular segments of sexually immature and mature rats. Sertoli cells prepared from sexually immature rats show progressive 125I-Pin binding in primary cultures that correlates with isoproterenol-induced cell shape changes, redistribution of immunoreactive vimentin, and phosphorylation of this intermediate filament protein. The development of 125I-Pin binding to Sertoli cell lysates is blocked by cycloheximide. Seminiferous tubules do not show significant isoproterenol-dependent vimentin phosphorylation nor 125I-Pin binding. However, vimentin phosphorylation can be induced by follicle-stimulating hormone or a cyclic nucleotide analog. This study stresses the need for correlating pharmacological-induced responses observed in Sertoli cell primary cultures with those in the intact seminiferous tubule.
DPDERL_viewshed_algorithm
Creators: name: Tang, Guoqing
Publisher: name: figshare
Year: 2024
Subjects: classificationCode: 370402
subject: Earth and space science informatics

description: Viewshed analyses using Digital Elevation Models (DEMs) can provide decision support information in a variety of areas. Traditional viewshed analyses are mainly based on a circular visual range centred on an observation point, and lack the ability to dynamically adjust the visual range by view angle. In addition, these algorithms face challenges in achieving a balance between computational efficiency and accuracy. To solve the above problems, this study proposes a field-of-view (FOV) angular range based partitioning strategy to realise dynamic range proximity-direction-elevation reference lines (DPDERL), which improves the adaptability to complex terrains and the flexibility of viewpoint application environments. After partitioning, the algorithm is optimised for grid point search and reference line update. Experimental results show that the algorithm takes less than half the time of the Xdraw algorithm for a variety of terrain regions, has an error of no more than 5% compared to the R3 algorithm, and has less aggregation of error points than the PDERL and the XPDERL. The DPDERL approach provides an effective solution for large-scale dynamic range viewshed analysis by balancing speed and accuracy.
Visualization and thermodynamic encoding of single-molecule partition functions
Creators: name: Palma, Carlos-Andres
name: Björk, Jonas
name: Klappenberger, Florian
name: Arras, Emmanuel
name: Kühne, Dirk
name: Stafström, Sven
name: Barth, Johannes V.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Materials Science (cond-mat.mtrl-sci)
subject: Statistical Mechanics (cond-mat.stat-mech)
subject: Chemical Physics (physics.chem-ph)
subject: FOS: Physical sciences

description: Ensemble averaging of molecular states is fundamental for the experimental determination of thermodynamic quantities. A special case occurs for single-molecule investigations under equilibrium conditions, for which free energy, entropy and enthalpy at finite-temperatures are challenging to determine with ensemble-averaging alone. Here, we provide a method to access single-molecule thermodynamics, by confining an individual molecule to a nanoscopic pore of a two-dimensional metal-organic nanomesh, where we directly record finite-temperature time-averaged statistical weights using temperature-controlled scanning tunneling microscopy. The obtained patterns represent a real space equilibrium probability distribution. We associate this distribution with a partition function projection to assess spatially resolved thermodynamic quantities, by means of computational modeling. The presented molecular dynamics based Boltzmann weighting model is able to reproduce experimentally observed molecular states with high accuracy. By an in-silico customized energy landscape we demonstrate that distinct probability distributions can be encrypted at different temperatures. Such modulation provides means to encode and decode information into position-temperature space or to realize nanoscopic thermal probes.
description: 20 Pages Main text, 5 Figures. 10 Pages Annexed text
Supplementary file for ’Cardiac Deficiency of P21-activated Kinase 1 Promotes Atrial Arrhythmogenesis in Mice following Adrenergic Challenge’
Creators: name: Jung, Eunjeong
name: Capel, Rebecca
name: Jiang, Congshan
name: Venturi, Elisa
name: Neagu, Georgiana
name: Pearcey, Sarah
name: Zhou, Yafei
name: Zhang, Yanmin
name: Lei, Ming
Publisher: name: The Royal Society
Year: 2023
Subjects: subject: Molecular Biology
subject: Physiology
subject: FOS: Biological sciences

description: P21-activated kinase 1 (Pak1) signalling plays a vital and overall protective role in the heart. However, the phenotypes of Pak1 deficiency in the cardiac atria have not been well explored. In this study, Pak1 cardiac-conditional knock-out (cKO) mice were studied under baseline and adrenergic challenge conditions. Pak1 cKO mice show atrial arrhythmias including atrial fibrillation (AF) in vivo, detected during anaesthetized electrocardiography without evidence of interstitial fibrosis upon Masson's trichrome staining. Optical mapping of left atrial preparations from Pak1 cKO mice revealed a higher incidence of Ca2+ and action potential alternans under isoprenaline challenge and differences in baseline action potential and calcium transient characteristics. Ryr2 channels from Pak1 cKO hearts had a higher open probability than those from wild-type. RT-qPCR and Western blotting indicated that pCamkIIδ and Ryr2 are highly phosphorylated at baseline in the atria of Pak1 cKO mice, while the expression of Slc8a2 and Slc8a3 as Na+–Ca2+ exchanger (NCX), controlling the influx of Ca2+ from outside of cell and efflux of Na+ from the cytoplasm, are augmented. ChIP study showed that pCreb1 interacts with Slc8a2 and Slc8a3. Our study thus demonstrates that deficiency of Pak1 promotes atrial arrhythmogenesis under adrenergic stress, likely through posttranslational and transcriptional modifications of key molecules that are critical to Ca2+ homeostasis.This article is part of the theme issue ‘The heartbeat: its molecular basis and physiological mechanisms’.
title: Climate change adaptation in UK seafood: Understanding and responding to climate change in the UK wild capture seafood industry
Creators: name: Garrett, A.
name: Buckley, P.
name: Brown, S.
name: Townhill, B.
Publisher: name: Marine Climate Change Impacts Partnership (MCCIP), Lowestoft, UK
Year: 2015

description: This summary report is focussed on climate change adaptation for the UK wild capture seafood industry. It considers the major industry impacts arising from key climate change drivers and sets out major areas where adaptation action may be required.
description: Seafood Strategic Outlook
Neutron Density Distributions of Neutron-Rich Nuclei Studied with the Isobaric Yield Ratio Difference
Creators: name: Ma, Chun-Wang
name: Bai, Xiao-Man
name: Yu, Jiao
name: Wei, Hui-Ling
Publisher: name: arXiv
Year: 2014
Subjects: subject: Nuclear Theory (nucl-th)
subject: Nuclear Experiment (nucl-ex)
subject: FOS: Physical sciences

description: The isobaric yield ratio difference (IBD) between two reactions of similar experimental setups is found to be sensitive to nuclear density differences between projectiles. In this article, the IBD probe is used to study the density variation in neutron-rich $^{48}$Ca. By adjusting diffuseness in the neutron density distribution, three different neutron density distributions of $^{48}$Ca are obtained. The yields of fragments in the 80$A$ MeV $^{40, 48}$Ca + $^{12}$C reactions are calculated by using a modified statistical abrasion-ablation model. It is found that the IBD results obtained from the prefragments are sensitive to the density distribution of the projectile, while the IBD results from the final fragments are less sensitive to the density distribution of the projectile.
description: 3 figures
Serotonin neurons on the ventral brain surface.
Creators: name: Chan-Palay, V.
name: Liposits, Z.
name: Palay, S. L.
name: Gorcs, T. J.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1985

description: Serotonin neurons and fibers on the subpial surface of the ventral medulla oblongata in the rat are described by immunohistochemistry and autoradiography. The neurons are concentrated in the area encompassed by the origins of the abducens, hypoglossal, glossopharyngeal, and vagus nerves. The highest number of serotonin surface neurons appears along the median medullary fissure or basilar sulcus, where they may represent the most ventral extensions of the raphe pallidus group. As these cells lie on the surface of the brain, they could be directly affected by alterations in the chemical composition of the cerebrospinal fluid and, depending on their connections, could influence important medullary functions.
LIBER FIM4L Recommendations 2020 v.01
Creators: name: Westerbeke, Jos
name: Pavlik, Jiri
name: Gietz, Peter
name: Teeuwen, Raoul
name: (Editors)
Publisher: name: Zenodo
Year: 2020
Subjects: Federated Identity Management
Library e-resource authentication
Federated SSO
Federated authentication

description: Publishers and suppliers of licensed online resources want to provide authorized users of institutions for higher education and research with access to their services in a controlled way. This document aims to function as a reference for libraries and publishers who want to set up an SSO connection.
POLAND - Migration and demographic patterns in Central-Eastern Europe
Creators: name: Brzozowski, Jan
name: Chwat, Olivia
name: Sikorska, Joanna
Publisher: name: Zenodo
Year: 2023
Subjects: poland
migration
migration scenarios

description: This report is a part of deliverable “D.6.2. Report on migration and demographic patterns in the EU CEE countries and potential source countries” from the project FUME – Future Migration Scenarios for Europe (870649), financed with the Horizon 2020 programme. In particular, this country report focuses on critical analysis of immigration data from Poland. The analysis consists of an overview of stock and flow data on migrants, including such dimensions as age groups, gender, country of origin, education levels and length of residence. This report is a first step in the analytical exercise of this deliverable which aims to determine migration potential from and to Poland and furthermore, to provide necessary data input for fine-tuning of the FUME migration projection model.
 Poland as the biggest economy of the Central and Eastern Europe region is an example of a migration transition country. In just 5 years, Poland has changed from an intensive labor-exporting country into one of major destination countries for third-country nationals in the EU. This report presents the historical framework of this transformation, major national groups of immigrants and their demographic structure. It also provides critical analysis on the validity of various statistical sources of data, and draws some initial conclusions on the temporality/permanent nature of immigrants in Poland.
Incidence of $q$-statistics in rank distributions
Creators: name: Yalcin, G. Cigdem
name: Robledo, Alberto
name: Gell-Mann, Murray
Publisher: name: arXiv
Year: 2014
Subjects: subject: Statistical Mechanics (cond-mat.stat-mech)
subject: FOS: Physical sciences

description: We show that size-rank distributions with power-law decay (often only over a limited extent) observed in a vast number of instances in a widespread family of systems obey Tsallis statistics. The theoretical framework for these distributions is analogous to that of a nonlinear iterated map near a tangent bifurcation for which the Lyapunov exponent is negligible or vanishes. The relevant statistical-mechanical expressions associated with these distributions are derived from a maximum entropy principle with the use of two different constraints, and the resulting duality of entropy indexes is seen to portray physically relevant information. While the value of the index $α$ fixes the distribution's power-law exponent, that for the dual index $2-α$ ensures the extensivity of the deformed entropy.
description: Santa Fe Institute working paper: http://www.santafe.edu/media/workingpapers/14-07-024.pdf. see: http://www.pnas.org/content/early/2014/09/03/1412093111.full.pdf+html
Specific regions of the intervening sequences of beta-globin RNA are resistant to nuclease in 50S heterogeneous nuclear RNA-protein complexes.
Creators: name: Patton, J. R.
name: Chae, C. B.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1985

description: The specific assembly of heterogeneous nuclear RNA-protein complexes (hnRNPs) containing precursor beta-globin RNA was investigated by using the 50S hnRNP released from chicken reticulocyte nuclei by endogenous nuclease. The nuclease-resistant regions were mapped on adult beta-globin intervening sequences (IVS) at the resolution of nucleotides with an RNA mapping method [Patton, J. R. and Chae, C.-B. (1983) J. Biol. Chem. 258, 3991-3995]. We found that there is one 28-nucleotide-long nuclease-resistant region in the first IVS and there are four nuclease-resistant regions in the second IVS. Of particular interest is the presence in 50S hnRNP of a nuclease-resistant region (24-28 nucleotides long) in both IVS immediately upstream from the putative lariat branch site in an RNA splicing intermediate. Our results demonstrate that hnRNPs containing precursor beta-globin RNA are, like those containing mature beta-globin RNA, assembled in a site-specific manner.
Toward Complete Statistics of Massive Binary Stars: Penultimate Results from the Cygnus OB2 Radial Velocity Survey
Creators: name: Kobulnicky, Henry A.
name: Kiminki, Daniel C.
name: Lundquist, Michael J.
name: Burke, Jamison
name: Chapman, James
name: Keller, Erica
name: Lester, Kathryn
name: Rolen, Emily K.
name: Topel, Eric
name: Bhattacharjee, Anirban
name: Smullen, Rachel A.
name: Alvarez, Carlos A. Vargas
name: Runnoe, Jessie C.
name: Dale, Daniel A.
name: Brotherton, Michael M.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Solar and Stellar Astrophysics (astro-ph.SR)
subject: FOS: Physical sciences

description: We analyze orbital solutions for 48 massive multiple-star systems in the Cygnus OB2 Association, 23 of which are newly presented here, to find that the observed distribution of orbital periods is approximately uniform in log P for P<45 d, but it is not scale-free. Inflections in the cumulative distribution near 6 d, 14, d, and 45 d, suggest key physical scales of ~0.2, ~0.4, and ~1 A.U. where yet-to-be-identified phenomena create distinct features. No single power law provides a statistically compelling prescription, but if features are ignored, a power law with exponent beta = -0.22 provides a crude approximation over P=1.4 -- 2000 d, as does a piece-wise linear function with a break near 45 d. The cumulative period distribution flattens at P > 45 d, even after correction for completeness, indicating either a lower binary fraction or a shift toward low-mass companions. A high degree of similarity (91% likelihood) between the Cyg OB2 period distribution and that of other surveys suggests that the binary properties at P<25 d are determined by local physics of disk/clump fragmentation and are relatively insensitive to environmental and evolutionary factors. Fully 30% of the unbiased parent sample is a binary with period P < 45 d. Completeness corrections imply a binary fraction near 55% for P < 5000 d. The observed distribution of mass ratios 0.2
description: Accepted for publication in the Astrophysical Journal
Two separate functions of class II (Ia) molecules: T-cell stimulation and B-cell excitation.
Creators: name: Haughton, G.
name: Corley, R. B.
name: LoCascio, N. J.
name: Ovnic, M.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1985

description: We have evaluated the role of major histocompatibility complex-encoded class II (Ia) molecules as transmembrane signaling receptors in the T helper cell-dependent activation of B lymphocytes. For these studies, we utilized the murine B-cell lymphoma CH12, which expresses both I-A and I-E class II molecules. In addition, CH12 cells carry IgM of known antigen specificity and require both specific antigen and Ia-restricted T-cell help for the induction of antibody secretion. In this respect, they resemble normal resting B cells. We have studied the ability of antigen-specific or alloreactive T helper cells reactive with either the I-A or the I-E molecules on CH12 to be activated and their ability to stimulate antibody production by CH12. The results show that, although CH12 cells present antigen to T helper cells that interact with either the I-A or the I-E molecules, CH12 cells are stimulated to secrete antibody only by T helper cells reactive with their I-E molecules. Our data demonstrate that class II molecules are transducers of signals for B-cell excitation in addition to serving a restricting function for helper T-cell stimulation. Moreover, the data demonstrate that these two functions, T-cell stimulation and B-cell excitation, are discrete and need not be expressed by the same Ia molecule.
Images of Gravitational and Magnetic Phenomena Derived from 2D Back-Projection Doppler Tomography of Interacting Binary Stars
Creators: name: Richards, Mercedes T.
name: Cocking, Alexander S.
name: Fisher, John G.
name: Conover, Marshall J.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Solar and Stellar Astrophysics (astro-ph.SR)
subject: FOS: Physical sciences

description: We have used 2D back-projection Doppler tomography as a tool to examine the influence of gravitational and magnetic phenomena in interacting binaries which undergo mass transfer from a magnetically-active star onto a non-magnetic main sequence star. This multi-tiered study of over 1300 time-resolved spectra of 13 Algol binaries involved calculations of the predicted dynamical behavior of the gravitational flow and the dynamics at the impact site, analysis of the velocity images constructed from tomography, and the influence on the tomograms of orbital inclination, systemic velocity, orbital coverage, and shadowing. The H$α$ tomograms revealed eight sources: chromospheric emission, a gas stream along the gravitational trajectory, a star-stream impact region, a bulge of absorption or emission around the mass-gaining star, a Keplerian accretion disk, an absorption zone associated with hotter gas, a disk-stream impact region, and a hot spot where the stream strikes the edge of a disk. We described several methods used to extract the physical properties of the emission sources directly from the velocity images, including S-wave analysis, the creation of simulated velocity tomograms from hydrodynamic simulations, and the use of synthetic spectra with tomography to sequentially extract the separate sources of emission from the velocity image. In summary, the tomography images have revealed results that cannot be explained solely by gravitational effects: chromospheric emission moving with the mass-losing star, a gas stream deflected from the gravitational trajectory, and alternating behavior between stream state and disk state. Our results demonstrate that magnetic effects cannot be ignored in these interacting binaries.
description: 18 pages, 4 tables, 12 figures; Astrophysical Journal, Vol. 794 (October 20, 2014)
LIBER FIM4L Recommendations 2022 v.2
Creators: name: Westerbeke, Jos
name: Pavlik, Jiri
name: Gietz, Peter
name: (Editors)
Publisher: name: Zenodo
Year: 2022
Subjects: Federated Identity Management
Library e-resource authentication
Federated SSO
Federated authentication

description: Publishers and suppliers of licensed online resources want to provide authorized users of institutions for higher education and research with access to their services in a controlled way. This document aims to function as a reference for libraries and publishers who want to set up an SSO connection.
A complete library of point substitution mutations in the glucocorticoid response element of mouse mammary tumor virus.
Creators: name: Vogt, K.
name: Edgell, M. H.
name: Nordeen, S. K.
name: Hutchison, C. A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1986

description: The glucocorticoid response element (GRE) of mouse mammary tumor virus (MMTV) was chemically synthesized as two complementary DNA strands bearing cohesive termini. During automated synthesis, random mutations were introduced into the DNA by "doping" each of the four nucleoside phosphoramidites (A, G, C, and T) with a low level of the other three. These preparations were annealed and cloned into an M13 phage vector to produce a library of GRE mutants. Mutations within the synthesized region were identified by sequencing phage isolates at random. All of the chemically distinct classes of transition and transversion mutations have been observed. Statistical considerations indicate that the library contains all of the possible 90 point substitution mutations within a 30-nucleotide mutagenic target. So far 88 of these substitutions have been isolated, 74 as single mutants. At least two of the three possible single mutants at each of the 30 positions have been identified.
A planetary system and a highly eccentric brown dwarf around the giant stars HIP67851 and HIP97233
Creators: name: Jones, M. I.
name: Jenkins, J. S.
name: Rojo, P.
name: Melo, C. H. F.
name: Bluhm, P.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Earth and Planetary Astrophysics (astro-ph.EP)
subject: FOS: Physical sciences

description: Context: So far more than 60 substellar companions have been discovered around giant stars. These systems present physical and orbital properties that contrast to those detected orbiting less evolved stars. Aims: We are conducting a radial velocity survey of 166 bright giant stars in the southern hemisphere. The main goals of our project are to detect and characterize planets in close-in orbits around giant stars in order to study the effects of the host star evolution on their orbital and physical properties. Methods: We have obtained precision radial velocities for the giant stars HIP67851 and HIP97233 that have revealed periodic signals, which are most likely induced by the presence of substellar companions. Results: We present the discovery of a planetary system and an eccentric brown dwarf orbiting the giant stars HIP67851 and HIP97233, respectively. The inner planet around HIP67851 has a period of 88.8 days, a projected mass of 1.4 Mjup and an eccentricity of 0.09. After Kepler 91b, HIP67851b is the closest-in known planet orbiting a giant star. Although the orbit of the outer object is not fully constrained, it is likely a super-Jupiter. The brown dwarf around HIP97233 has an orbital period of 1058.8 days, a minimum mass of 20.0 Mjup and an eccentricity of 0.61. This is the most eccentric known brown dwarf around a giant star.
description: Accepted for publication in A&A
title: ORN ECube Data Format Specification
Creators: name: Cecconi, Baptiste
name: Viou, Cedric
name: Coffre, Andrée
Publisher: name: PADC/CDN
Year: 2022

description: The ECube data format is the output format of the high resolution digital receivers developed in Nançay Radio Observatory (ORN), and used for the Nançay Decameter Array (NDA) receivers:  NDA/NewRoutine, NDA/MEFISTO and NDA/JunoN. The ECube data files are composed of a header section followed by a series of ECube data records, as described in this document
Optimizing Hash-Based Signatures in Java
Creators: name: Rausch, Tim
Publisher: name: Karlsruher Institut für Technologie (KIT)
Year: 2023

description: Hash-based signature schemes are an extensively studied and well-understood choice for quantum-safe digital signatures. However, certain operations, most notably the key generation, can be comparably expensive. It is, therefore, essential to use well-optimized implementations. This thesis aims to explore, implement, and evaluate optimization strategies for hashbased signature implementations in Java. These include the use of special hardware features like vector instructions and hardware acceleration for hash functions as well as the parallelization of the key generation. Overall, we are able to reduce the time required for an XMSS key generation with SHA-2 by up to 96.4% (on four CPU cores) compared to the unmodified BouncyCastle implementation. For SPHINCS+ with the Haraka hash function family, we achieve a reduction of up to 95.7% on only one CPU core. Furthermore, we investigate the use of two scheme variants WOTS-BR and WOTS+C proposed in the literature for verification-optimized signatures. We improve the existing theoretical analysis of both, provide a comparison and experimentally validate our improved theoretical analysis.
Exact determination of the volume of an inclusion in a body having constant shear modulus
Creators: name: Thaler, Andrew E.
name: Milton, Graeme W.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Analysis of PDEs (math.AP)
subject: Mathematical Physics (math-ph)
subject: FOS: Mathematics
subject: FOS: Physical sciences
subject: 74B05, 35Q74

description: We derive an exact formula for the volume fraction of an inclusion in a body when the inclusion and the body are linearly elastic materials with the same shear modulus. Our formula depends on an appropriate measurement of the displacement and traction around the boundary of the body. In particular, the boundary conditions around the boundary of the body must be such that they mimic the body being placed in an infinite medium with an appropriate displacement applied at infinity.
Contrasting levels of transferrin gene activity in cultured rat Sertoli cells and intact seminiferous tubules.
Creators: name: Kierszenbaum, A. L.
name: Lee, N. T.
name: Chae, C. B.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1986

description: Two-dimensional polyacrylamide gel electrophoresis of proteins and transfer blot hybridization of RNA have been used to study the activity and expression of the rat transferrin gene in cultured Sertoli cells and in whole testis and isolated seminiferous tubules of sexually immature and mature rats. Although the transferrin gene in cultured Sertoli cells is actively engaged in the transcription of mRNA and the mRNA is translated into a secretory product, little transferrin mRNA and transferrin protein are present in whole testes and isolated seminiferous tubules. Sertoli cells upon culturing show a time-dependent transferrin gene activation, and abundant transferrin mRNA can be detected 6 hr after plating. A similar study using intact seminiferous tubular segments from the same rats failed to show a comparable temporal activation of the transferrin gene. Results of this study, together with previous experimental data, suggest that Sertoli cells in vivo are most likely not actively engaged in the synthesis of a testicular transferrin but, instead, rely mainly on plasma transferrin contributed by the liver. In vitro, Sertoli cells, released from the physiological constraints that operate in vivo, rapidly activate the transferrin gene, resulting in abundant newly synthesized Sertoli cell transferrin product.
title: Front Matter, Table of Contents, Preface, Conference Organization
Creators: name: Böhme, Rainer
name: Kiffer, Lucianna
Publisher: name: Schloss Dagstuhl – Leibniz-Zentrum für Informatik
Year: 2024
Subjects: subject: Front Matter
subject: Table of Contents
subject: Preface
subject: Conference Organization
subject: Security and privacy → Mathematical foundations of cryptography
subject: Theory of computation → Cryptographic primitives
subject: Theory of computation → Cryptographic protocols
subject: Security and privacy → Distributed systems security
subject: Security and privacy → Privacy-preserving protocols
subject: Security and privacy → Pseudonymity, anonymity and untraceability
subject: Security and privacy → Economics of security and privacy
subject: Theory of computation → Algorithmic game theory and mechanism design
subject: Applied computing → Economics
subject: Applied computing → Digital cash

description: Front Matter, Table of Contents, Preface, Conference Organization
description: LIPIcs, Vol. 316, 6th Conference on Advances in Financial Technologies (AFT 2024), pages 0:i-0:xxii
Screw-Dislocation-Driven Growth of Two-Dimensional Few-Layer and Pyramid-Like WSe2 by Sulfur-Assisted Chemical Vapor Deposition
Creators: name: Chen, Liang
name: Liu, Bilu
name: Abbas, Ahmad N.
name: Ma, Yuqiang
name: Fang, Xin
name: Liu, Yihang
name: Zhou, Chongwu
Publisher: name: arXiv
Year: 2014
Subjects: subject: Materials Science (cond-mat.mtrl-sci)
subject: Mesoscale and Nanoscale Physics (cond-mat.mes-hall)
subject: FOS: Physical sciences

description: Two-dimensional (2D) layered tungsten diselenides (WSe2) material has recently drawn a lot of attention due to its unique optoelectronic properties and ambipolar transport behavior. However, direct chemical vapor deposition (CVD) synthesis of 2D WSe2 is not as straightforward as other 2D materials due to the low reactivity between reactants in WSe2 synthesis. In addition, the growth mechanism of WSe2 in such CVD process remains unclear. Here we report the observation of a screw-dislocation-driven (SDD) spiral growth of 2D WSe2 flakes and pyramid-like structures using a sulfur-assisted CVD method. Few-layer and pyramid-like WSe2 flakes instead of monolayer were synthesized by introducing a small amount of sulfur as a reducer to help the selenization of WO3, which is the precursor of tungsten. Clear observations of steps, helical fringes, and herring-bone contours under atomic force microscope characterization reveal the existence of screw dislocations in the as-grown WSe2. The generation and propagation mechanisms of screw dislocations during the growth of WSe2 were discussed. Back-gated field-effect transistors were made on these 2D WSe2 materials, which show on/off current ratios of 106 and mobility up to 44 cm2/Vs.
Cumulative CO2 Emissions of International Transport
Creators: name: Nowarski, Joseph
Publisher: name: figshare
Year: 2022
Subjects: subject: 40104 Climate Change Processes
subject: FOS: Earth and related environmental sciences
subject: Climate Science

description: The dataset of global cumulative CO2 emissions is from 1750. The baseline of this dataset is 1749. The dataset includes also international transport cumulative CO2 emissions from 1950. The baseline of international transport is 1949. This work considers the year 1783, when the first steamship was built, as the first year of the international transport CO2 emissions. The global cumulative CO2 emissions including international transport are converted to the 1875 baseline, similar to the Global Warming baseline (1850-1900). The international transport cumulative CO2 emissions caused a 0.032°C temperature increase in 2020, 2.5% of the Global Warming.