title: Ð¡Ð²Ð¾Ð¹ÑÑÐ²Ð° Ð±ÐµÐ»ÐºÐ° SMC Ureaplasma parvum
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: The properties of SMC protein of Ureaplasma parvum cell
Creators: name: Ð¨ÑÑÐ¾Ð², ÐÐ»Ð°Ð´Ð¸Ð¼Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð¼Ð¸ÐºÐ¾Ð¿Ð»Ð°Ð·Ð¼Ñ
Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ
mycoplasmas
activity

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ ÑÐ²Ð¾Ð¹ÑÑÐ² Ð±ÐµÐ»ÐºÐ° SMC U. parvum, Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸ ÐºÐ¾ÑÐ¾ÑÐ¾Ð³Ð¾ ÑÑÐ°ÑÑÐ²ÑÑÑ Ð² ÐºÐ¾Ð¼Ð¿Ð°ÐºÑÐ¸Ð·Ð°ÑÐ¸Ð¸ Ð² Ð¿ÑÐ¾ÑÑÑÐ°Ð½ÑÑÐ²Ðµ Ð³ÐµÐ½Ð¾Ð¼Ð½Ð¾Ð¹ ÐÐÐ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹. ÐÑÐµÐ´Ð¿Ð¾Ð»Ð¾Ð¶Ð¸ÑÐµÐ»ÑÐ½Ð¾, SMC U. parvum ÑÐ²Ð»ÑÐµÑÑÑ Ð¾Ð´Ð½Ð¸Ð¼ Ð¸Ð· ÐºÐ»ÑÑÐµÐ²ÑÑ ÑÐµÑÐ¼ÐµÐ½ÑÐ¾Ð² Ð´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¼Ð¸ÐºÐ¾Ð¿Ð»Ð°Ð·Ð¼ÐµÐ½Ð½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº. Ð ÑÐ¾Ð´Ðµ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¸ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ñ ÑÑÐ°Ð½Ð´Ð°ÑÑÐ½ÑÐµ Ð±Ð¸Ð¾ÑÐ¸Ð¼Ð¸ÑÐµÑÐºÐ¸Ðµ Ð¼ÐµÑÐ¾Ð´Ñ Ð¸ ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð°Ð½Ð°Ð»Ð¸Ð· ÑÐ´Ð²Ð¸Ð³Ð° ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¿Ð¾Ð´Ð²Ð¸Ð¶Ð½Ð¾ÑÑÐ¸ ÐÐÐ Ð² Ð¿ÑÐ¸ÑÑÑÑÑÐ²Ð¸Ð¸ SMC U. parvum. ÐÐ¾Ð¿Ð¾Ð»Ð½Ð¸ÑÐµÐ»ÑÐ½Ð¾ Ð±ÑÐ»Ð¾ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¾ ÑÐ¿ÐµÐºÑÑÐ¾ÑÐ¾ÑÐ¾Ð¼ÐµÑÑÐ¸ÑÐµÑÐºÐ¾Ðµ Ð¸Ð·Ð¼ÐµÑÐµÐ½Ð¸Ðµ ÐÐ¢Ð¤-Ð°Ð·Ð½Ð¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ SMC U. parvum Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¼Ð°Ð»Ð°ÑÐ¸ÑÐ¾Ð²Ð¾Ð³Ð¾ Ð·ÐµÐ»ÐµÐ½Ð¾Ð³Ð¾. ÐÐµÐ»Ð¾Ðº SMC U. parvum Ð±ÑÐ» ÑÑÐ¿ÐµÑÐ½Ð¾ Ð½Ð°ÑÐ°Ð±Ð¾ÑÐ°Ð½ Ð¸ Ð¾ÑÐ¸ÑÐµÐ½. ÐÐ½Ð°Ð»Ð¸Ð· ÑÐ´Ð²Ð¸Ð³Ð° ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¿Ð¾Ð´Ð²Ð¸Ð¶Ð½Ð¾ÑÑÐ¸ ÐÐÐ Ð¿Ð¾Ð·Ð²Ð¾Ð»Ð¸Ð» Ð²ÑÑÐ²Ð¸ÑÑ Ð½Ð¾Ð²ÑÐµ ÐÐÐ-ÑÐ²ÑÐ·ÑÐ²Ð°ÑÑÐ¸Ðµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° SMC U. parvum. ÐÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð´Ð°Ð½Ð½ÑÐ¹ Ð±ÐµÐ»Ð¾Ðº Ð½ÐµÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐ½Ð¾ ÑÐ²ÑÐ·ÑÐ²Ð°ÐµÑÑÑ Ñ Ð»Ð¸Ð½ÐµÐ¹Ð½Ð¾Ð¹ Ð¸ ÐºÐ¾Ð»ÑÑÐµÐ²Ð¾Ð¹ Ð´Ð²ÑÑÐµÐ¿Ð¾ÑÐµÑÐ½Ð¾Ð¹ ÐÐÐ (Ð´ÑÐÐÐ) Ð² Ð¾ÑÑÑÑÑÑÐ²Ð¸Ð¸ ÐÐ¢Ð¤. ÐÐ¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸Ðµ ÐÐ¢Ð¤ Ð¸ ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ðµ ÐµÐ³Ð¾ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ Ð¿ÑÐ¸Ð²Ð¾Ð´Ð¸Ñ Ðº ÑÑÐ¸Ð»ÐµÐ½Ð¸Ñ ÑÐ²ÑÐ·ÑÐ²Ð°Ð½Ð¸Ñ Ð´Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° Ñ Ð¾Ð±Ð¾Ð¸Ð¼Ð¸ ÑÐ¸Ð¿Ð°Ð¼Ð¸ Ð´ÑÐÐÐ. ÐÑÐ»Ð¾ Ð¾ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸Ð·Ð¾Ð²Ð°Ð½Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ MgCl2, KCl, pH ÑÑÐµÐ´Ñ Ð½Ð° ÑÐ²ÑÐ·ÑÐ²Ð°Ð½Ð¸Ðµ Ð±ÐµÐ»ÐºÐ° SMC U. parvum Ñ Ð´ÑÐÐÐ. ÐÑÐ»Ð° Ð¾ÑÐµÐ½ÐµÐ½Ð° ÑÐºÐ¾ÑÐ¾ÑÑÑ Ð³Ð¸Ð´ÑÐ¾Ð»Ð¸Ð·Ð° ÐÐ¢Ð¤ Ð±ÐµÐ»ÐºÐ¾Ð¼ SMC U. parvum Ð² 6,2 Â± 0,3 Ð¼Ð¾Ð»ÐµÐºÑÐ» Ð² Ð¼Ð¸Ð½ÑÑÑ. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð²Ð¾ÑÑÑÐµÐ±Ð¾Ð²Ð°Ð½Ñ Ð² Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐ¸Ñ ÑÑÐ½Ð´Ð°Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸ÑÑ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½ÑÑ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð¾Ð² ÐºÐ¾Ð¼Ð¿Ð°ÐºÑÐ¸Ð·Ð°ÑÐ¸Ð¸ Ð² Ð¿ÑÐ¾ÑÑÑÐ°Ð½ÑÑÐ²Ðµ Ð³ÐµÐ½Ð¾Ð¼Ð½Ð¾Ð¹ ÐÐÐ Ð¸ Ð² ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐµ Ð»ÐµÐºÐ°ÑÑÑÐ²ÐµÐ½Ð½ÑÑ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð² Ð¿ÑÐ¾ÑÐ¸Ð² Ð¼Ð¸ÐºÐ¾Ð¿Ð»Ð°Ð·Ð¼ÐµÐ½Ð½ÑÑ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¹, Ð½Ð°ÑÐµÐ»ÐµÐ½Ð½ÑÑ Ð½Ð° Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð±ÐµÐ»ÐºÐ° SMC.
description: This work is devoted to studying the properties of U. parvum SMC protein, whose orthologs are involved in the compactization of bacterial genomic DNA in space. Presumably, SMC U. parvum is one of the key enzymes of mycoplasma cell division. During the work, standard biochemical methods and the specific DNA electrophoretic mobility shift assay in the presence of SMC U. parvum were used. Additionally, the spectrophotometric measurement of the ATPase activity of SMC U. parvum was carried out using malachite green. SMC U. parvum protein was successfully produced and purified. The electrophoretic mobility shift assay revealed new DNA-binding properties of SMC U. parvum. This protein has been shown to bind nonspecifically to linear and circular double-stranded DNA (dsDNA) in the absence of ATP. The addition of ATP and an increase in its concentration leads to increased binding of this protein to both types of dsDNA. The influence of MgCl2, KCl, and pH on the binding of SMC U. parvum to dsDNA was characterized. The rate of ATP hydrolysis by SMC U. parvum was estimated to be 6,2 Â± 0,3 molecules per minute. The results obtained are in demand in further fundamental studies of the molecular mechanisms of compactization of genomic DNA in space and in the development of drugs against mycoplasma infections aimed at inhibiting the SMC protein.