cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
title: Developing novel therapeutic strategies for targeting the p53 pathway in renal cell carcinoma
Creators: name: Ahmed-Ebbiary, Amro
Publisher: name: University of Liverpool Repository
Year: 2014

description: Renal cell carcinoma (RCC) is a notoriously difficult cancer to treat. RCC has an unpredictable course, is radio- and chemo-resistant and survival in response to treatment with current therapies for the metastatic disease is generally very poor. This underscores the necessity for novel therapeutics for this condition. The tumour suppressor p53 is rarely mutated in RCC and up-regulation of p53 and its negative regulator, MDM2, has recently been shown to be an independent prognostic indicator for patients with poor outcome. The function of p53 in RCC cells in culture appears to be partially intact and is regulated by MDM2. Rescue of p53 from the inhibitory effects of MDM2 using inhibitors of the p53-MDM2 interaction results in cell cycle arrest or senescence, not apoptosis, three cell fates that p53 can induce depending on the tissue or tumour type. Because of the potentially deleterious effects of senescent cells on the tissue microenvironment, it was decided that apoptosis would be the preferable cell fate for the therapeutic reactivation of p53 in RCC cells. To that end, the MDM2 inhibitor Nutlin-3 was combined with the Bcl-2 family inhibitor ABT-737. This combination of drugs synergistically inhibited the proliferation of RCC cells harbouring wild type p53 but not in cells harbouring mutant p53. This synergistic inhibition of proliferation was shown to be p53-dependent and was confirmed to be inducing apoptosis, but only in RCC cells that had high levels of p53 protein. The synergistic inhibition of proliferation was not recapitulated when an inhibitor of MDM2 ubiquitin ligase activity was combined with ABT-737. The combination of Nutlin-3 and ABT-737 induced pro-apoptotic Puma and Noxa in RCC cells and the apoptosis was shown to be substantially Bax-dependent. This study suggests that the combination of Nutlin-3 and ABT-737 should be considered as a candidate for pre-clinical development in the treatment of RCC.
Expression of multi-copy flavonoid pathway genes coincides with anthocyanin, flavonol and flavan-3-ol accumulation of grapevine
Creators: name: Jeong, S. T.
name: Goto-Yamamoto, N.
name: Hashizume, K.
name: Esaka, M.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: The biosynthetic pathways of main grape flavonoids: anthocyanins, flavonols, and flavan-3-ols, hold in common the early step enzymes of biosynthetic pathway: chalcone synthase (CHS), chalcone isomerase (CHI), and flavanone 3-hydroxylase (F3H), the genes of which are multi-copied in the grape genome. The ratios of mRNA levels of the three Chss (Chs1, Chs2, and Chs3), as well as those of the two Chis (Chi1 and Chi2) and those of the two F3hs (F3h1 and F3h2), were different among organs, even though no organ-specificity was observed in the strictest sense. Multiple regression analysis demonstrated that the transcription of particular genes significantly coincided with the biosynthesis of a particular flavonoid: the transcription of Chi2 coincided with flavan-3-ol; Chs1, Chs2, F3h1, and F3h2 with flavonol; and Chs2, Chs3, and F3h2 with anthocyanin biosynthesis. Thus, the transcription of these multi-copy genes is likely induced differently for the biosyntheses of anthocyanins, flavonols, and flavan-3-ols.
description: VITIS - Journal of Grapevine Research, Vol. 47 No. 3 (2008): Vitis
Molecular cloning of dihydroflavonol 4-reductase gene from grape berry and preparation of an anti-DFR polyclonal antibody
Creators: name: Zhang, Ping
name: Wen, Peng-Fei
name: Wan, Si-Bao
name: Wang, Wei
name: Pan, Qiu-Hong
name: Zhan, Ji-Chen
name: Huang, Wei-Dong
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Dihydroflavonol 4-reductase (DFR, EC 1.1.1.219) is a key enzyme of the flavonoid pathway, which synthesizes numerous secondary metabolites to determine the quality of grape berry and wine. The full-length dfr cDNA with 1014 bp was cloned from grape berry, and then introduced into an expressed plasmid pET-30a (+) vector at the EcoR I and Xho I restriction sites. With induction of the isopropyl-β-D-thiogalactoside (IPTG), the pET-dfr was highly expressed in Escherichia coli BL21 (DE3) pLysS cells. A fusion protein with the His-Tag was purified through Ni-NTA His Bind Resin and then used as the antigen to immunize a New Zealand rabbit. The resulting antiserum was further purified precipitated by 50 % saturated ammonium sulfate and DEAE-Sepharose FF chromatography to obtain the immunoglobulin G (IgG) fraction. The resulting polyclonal antibody was found capable of immuno-recognizing the DFR of the crude protein extracts from grape berry. This work undoubtedly provides the possibility for further studies on biological regulation of DFR activity in grape berry.
description: VITIS - Journal of Grapevine Research, Vol. 47 No. 3 (2008): Vitis
title: Prediction of bending wave transmission across coupled plates affected by spatial filtering and non-diffuse vibration fields
Creators: name: Wilson, David
Publisher: name: University of Liverpool Repository
Year: 2014

description: This thesis concerns models based on Statistical Energy Analysis (SEA) to predict bending wave vibration in heavyweight buildings from structure-borne sound sources such as machinery. These sources tend to inject most power in the low- and mid-frequency ranges where the walls and floors have low mode counts and low modal overlap for which calculated Coupling Loss Factors (CLFs) from semi-infinite plate theory can be in error. For machinery it is necessary to predict vibration on walls/floors that are remote from the source room. In this situation, propagation across successive structural junctions causes spatial filtering of the wave field and the assumption of a diffuse field in each plate subsystem breaks down. The predictive approach described in the European Standard EN12354 uses SEA path analysis which assumes that transmission is dominated by first-order paths. However the feasibility of extending the concept of path analysis to walls and floors of rooms that are distant from the source room(i.e. not adjacent) is unknown. These issues are addressed in the thesis. The feasibility of SEA path analysis was assessed by quantifying the total contribution to receiver subsystem energy from paths containing specified numbers of CLFs. For receiving subsystems which are attached directly to the source subsystem, the EN12354 approach was found to underestimate the energy levels. For rooms remote from the source room, path analysis was found to significantly underestimate the vibration of the walls/floors which form the receiver room. Alternative approaches to improve predictions in large heavyweight buildings were assessed through comparison with Monte-Carlo Finite Element Method (MCFEM) models which were validated on a small heavyweight building. Matrix SEA was used with CLFs calculated for L-, T- and X-junctions using analytical models for rectangular plates to try and incorporate modal features. For isolated junctions, there was good agreement with MCFEM but in large buildings. However, it was unable to predict the peaks and troughs in the vibration response to one-third octave band accuracy although it can estimate the envelope response for plates that are directly connected to the source plate. In general, matrix SEA using finite plate theory CLFs does not improve the prediction in one-third octave bands when the statistical mode count is less than unity. Ray tracing was therefore investigated which showed that the angular distribution of power incident on the plate edges differed significantly from a diffuse field. Computationally efficient ray tracing was then developed for inclusion in Advanced SEA (ASEA) models to account for indirect coupling between plate subsystems. ASEA gave significant improvements over matrix SEA when there were large numbers of structural junctions between the source and receiving plates.
Expression of genes associated with anthocyanin synthesis in red-purplish, pink, pinkish-green and green grape berries from mutated 'Sangiovese' biotypes: A case study
Creators: name: Ramazzotti, S.
name: Fillipetti, I.
name: Intrieri, C.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Using normal red-purplish grape bunches and pink, pink-green and green berry colour-mutated biotypes of cv. Sangiovese (V. vinifera L.), we investigated their anthocyanin metabolism via biochemical and molecular assays. The number and composition of the different types of anthocyanins were analysed by spectrophotometry and chromatography. The expression of six structural genes of the biosynthetic pathway (chalcone synthase [CHS], chalcone isomerase [CHI], flavanon-3-hydroxylase [F3H], dihydroflavonol 4-reductase [DFR], leucoanthocyanidin dioxygenase [LDOX] and UDP-glucose 3-O-flavonoid:glucosyltransferase [UFGT]) was determined over the four weeks subsequent to veraison via Northen blot and Real Time PCR. The grapes from the non-mutated biotype showed a prevailing accumulation of monoglycoside anthocyanin fractions, with only traces of acetyl and p-coumaroyl derivatives. The berries of the mutated biotypes showed a gradual berry pigment loss associated with a reduction in total anthocyanin content, although anthocyanin composition was the same of the non-mutant biotype. Indeed, the Northern blot assay data, as confirmed by the quantitative Real Time PCR tests, showed a differential expression in the berries of the non mutated and mutated biotypes for the UFGT gene, proving normal in the red-purplish, lower in the biotypes with pink and pink-green berries and wholly lacking in the green one. Thus, the UFGT gene in berry skin of colour-mutated 'Sangiovese' biotypes is controlled independently of the other structural genes encoding enzymes in the anthocyanin biosynthetic pathway and its capacity of expression is a critical factor in the synthesis and storage of these compounds.
description: VITIS - Journal of Grapevine Research, Vol. 47 No. 3 (2008): Vitis
Structure of the Texas Orocline beneath the sedimentary cover (southeast Queensland, Australia)
Creators: name: S. Brooke-Barnett
name: G. Rosenbaum
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences

description: The New England Orogen in eastern Australia is characterised by orogenic-scale curvatures (oroclines). The largest and most prominent curvature in this system is the Texas Orocline, but its subsurface geometry is still poorly constrained. A large component of the orocline is covered by post-oroclinal sedimentary rocks, which obscure deeper sections of the orocline and make it difficult to understand how the structure is connected to other segments of the New England Orogen. Here, we present geophysical data that elucidate the structure of the Texas Orocline below the sedimentary cover. Using 2D seismic, aeromagnetic TMI (total magnetic intensity) and Bouguer gravity datasets, in combination with outcrop and well data, we identified the depth to the New England ‘basement’ and significant faults intersecting it. We also traced the strongly contorted subsurface continuation of the Peel-Yarrol Fault System, which is characterised by local gravity and magnetic anomalies associated with isolated serpentinite outcrops. Constraints on the timing of oroclinal bending were obtained from the interpretation of seismic transects, which showed that early Permian sedimentary rocks of the Bowen Basin were deposited in a subtrough that deviates from the general north–south trend of the Bowen Basin. The subtrough is oriented approximately parallel to the western limb of the Texas Orocline, thus suggesting that the orocline formed during and/or after early Permian rifting. Our analysis indicates that initial bending occurred contemporaneously with the development of the early Permian rift basins, most likely in the backarc region of a retreating subduction zone. Subsequently, phases of strike-slip and contractional deformation have further tightened the pre-existing curvatures.
Virgichneumon albosignatus
Creators: name: Riedel
name: Pénigot
name: Schwarz
name: Diller
name: Johansson
name: Japoshvili, G.
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hymenoptera
Ichneumonidae
Virgichneumon
Virgichneumon albosignatus

description: Virgichneumon albosignatus (GRAVENHORST, 1829)
M a t e r i a l e x a m i n e d: Trap 4: 1♀ 20.iv.-5.v.
D i s t r i b u t i o n: Transpalaearctic, known from Georgia (RIEDEL et al. 2018).
description: Published as part of Riedel, Pénigot, Schwarz, Diller, Johansson & Japoshvili, G., 2023, Darwin wasps (Hymenoptera, Ichneumonidae) of the Kintrishi National Park, Sakartvelo (Georgia), with descriptions of six new species, pp. 61-153 in Linzer biologische Beiträge 55 (1) on page 107, DOI: 10.5281/zenodo.10788481
title: Vibrotactile perception of musical pitch
Creators: name: Mate-Cid, Saul
Publisher: name: University of Liverpool Repository
Year: 2014

description: Previous vibrotactile research has provided little or no definitive results on the discrimination and identification of important pitch aspects for musical performance such as relative and absolute pitch. In this thesis, psychophysical experiments using participants with and without hearing impairments have been carried out to determine vibrotactile detection thresholds on the fingertip and foot, as well as assess the perception of relative and absolute vibrotactile musical pitch. These experiments have investigated the possibilities and limitations of the vibrotactile mode for musical performance. Over the range of notes between C1 (32.7Hz) and C6 (1046.5Hz), no significant difference was found between the mean vibrotactile detection thresholds in terms of displacement for the fingertip of participants with normal hearing and with severe/profound hearing impairments. These thresholds have been used to identify an optimum dynamic range in terms of frequency-weighted acceleration to safely present vibrotactile music. Assuming a practical level of stimulation ≈10dB above the mean threshold, the dynamic range was found to vary between 12 and 27dB over the three-octave range from C2 to C5. Results on the fingertip indicated that temporal cues such as the transient and continuous parts of notes are important when considering the perception of vibrotactile pitch at suprathreshold levels. No significant difference was found between participants with normal hearing and with severe/profound hearing impairments in the discrimination of vibrotactile relative pitch from C3 to C5 using the fingertip without training. For participants with normal hearing, the mean percentage of correct responses in the post-training test was greater than 70% for intervals between four and twelve semitones using the fingertip and three to twelve semitones using the forefoot. Training improved the correct responses for larger intervals on fingertips and smaller intervals on forefeet. However, relative pitch discrimination for a single semitone was difficult, particularly with the fingertip. After training, participants with normal hearing significantly improved in the discrimination of relative pitch with the fingertip and forefoot. However, identifying relative and absolute pitch was considerably more demanding and the training sessions that were used had no significant effect.
Somatic embryogenesis and plant regeneration of Vitis vinifera cultivars 'Macabeo' and 'Tempranillo'
Creators: name: Cutanda, M. C.
name: Bouquet, A.
name: Chatelet, P.
name: Lopez, G.
name: Botella, O.
name: Montero, F. J.
name: Torregrosa, L.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Different experimental conditions have been compared to achieve a high efficiency in embryogenic calli initiation from 'Macabeo' and 'Tempranillo' anthers. Specifically, two stages of anther development were tested (corresponding to tetrad cells or uninucleate pollen), and direct culture of anthers was compared to culture after a cold treatment of inflorescences (4 °C during 48 h). In addition, two induction media (C1 P and B2), mainly differing by microelement and cytokinin levels, were evaluated. Experiment repeatability was also examined with a repetition of anther culture one week later. Callus initiation was similar in all media and treatments for both cultivars, usually starting from the anther filament. A simple protocol for efficient induction of embryogenesis in 'Macabeo' and 'Tempranillo' consisted in: • selecting the first inflorescence from hardwood cutting, • excising anthers at uninucleate pollen stage without cold treatment of the inflorescences, • incubating anthers on C1 P medium. The procedure used for embryo germination and plant regeneration, allowed to obtain a conversion rate up to 75 % in 'Macabeo' and 60 % in 'Tempranillo'. The protocol proposed represents the first regeneration system developed for the Spanish cultivars 'Macabeo' and 'Tempranillo'.
description: VITIS - Journal of Grapevine Research, Vol. 47 No. 3 (2008): Vitis
Investigation of Flap Flexibility of β-Secretase Using Molecular Dynamic Simulations
Creators: name: Hezekiel M. Kumalo
name: Soumendranath Bhakat
name: Soliman, Mahmoud E.
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Computational Biology
subject: Biological Sciences
subject: Sociology
subject: FOS: Sociology
subject: Chemistry
subject: Biotechnology
subject: Pharmacology
subject: Molecular Biology
subject: Biochemistry
subject: Biophysics

description: Flap motif and its dynamics were extensively reported in aspartate proteases e.g. HIV proteases, and plasmepsins. Herein, we report the first account of flap dynamics among different conformations of β-secretase using molecular dynamics simulation. Various parameters were proposed and a selected few were picked which could appropriately describe the flap motion. Three systems were studied, namely Free (BACEFree) and two ligand-bound conformations, which belonged to space groups P6122 (BACEBound1) and C2221 (BACEBound2) respectively and four parameters (distance between the flaps tip residue, Thr72 and Ser325, d1; dihedral angle, ϕ (Thr72-Asp32-Asp228-Ser325); TriCα angles, θ1 (Thr72-Asp32-Ser325), and θ2 (Thr72-Asp228-Ser325)) were proposed to understand the change in dynamics of flap domain and the extent of flap opening and closing. Analysis of, θ2,d1,θ1 and ϕ confirmed that the BACEFree adopted semi-open, open and closed conformations with slight twisting during flap opening. However, BACEBound1 (P6122) showed an adaptation to open conformation due to lack hydrogen bond interaction between the ligand and flap tip residue. A slight flap twisting,ϕ (lateral twisting) was observed for BACEBound1 during flap opening which correlates with the opening of BACEFree. Contradictory to the BACEBound1, the BACEBound2 locked the flap in a closed conformation throughout the simulation due to formation of a stable hydrogen bond interaction between flap tip residue and ligand. Analyses of all three systems highlights that d1, θ2 and ϕ can be precisely used to describe the extent of flap opening and closing concurrently with snapshots along the molecular dynamics trajectory across several conformations of β-secretase.
Parentage of grapevine rootstock ‘Fercal’ finally elucidated
Creators: name: Laucou, V.
name: Boursiquot, J. M.
name: Lacombe, T.
name: Bordenave, L.
name: Decroocq, S.
name: Ollat, N.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Using a set of 20 microsatellite markers, ‘B.C. n°1B’ (mother) and ‘31 Richter’ (father) were demonstrated to be the true parents of ‘Fercal’ rootstock. ‘333 Ecole de Montpellier’ was definitively excluded as the putative father. ‘B.C. n°1A’ and ‘B.C. n°1B’ were shown to be distinct genotypes. ‘Ugni blanc’, and not ‘Colombard’, was discovered to be the Vitis vinifera father of ‘B.C. n°1B’.
description: VITIS - Journal of Grapevine Research, Vol. 47 No. 3 (2008): Vitis
Impact of citrus nematode (Tylenchulus semipenetrans) densities in soil on yield of grapevines (Vitis vinifera 'Shiraz') in south-eastern New South Wales
Creators: name: Rahman, L.
name: Creecy, H.
name: Orchard, B.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: 'Shiraz' is a popular red wine grape variety grown in NSW, Australia, and is susceptible to citrus nematode (Tylenchulus semipenetrans). The extent of damage, particularly yield loss, or the damage threshold level of T. semipenetrans in 'Shiraz' is not known. In this study we investigated the population dynamics and the effects of a range of population densities of T. semipenetrans on yield of 'Shiraz' in a naturally infested vineyard across three growing seasons. Results showed that the population density of T. semipenetrans J2 in soil did not increase or decrease consistently during the trial period. However, the population densities varied significantly (P < 0.05) between the temporal seasons in a year and were in order of summer > spring > autumn > winter. Yield from vines with T. semipenetrans J2 population densities greater than 9,000·kg-1 dry soil (average population over 11,000·kg-1 dry soil) was 15 % lower compared to vines with 500-3,000 (average 932) T. semipenetrans J2·kg-1 dry soil in 2004 but not in 2001 and 2003. Regression analysis showed a linear trend (r = -0.36) on yield decrease with the increase of T. semipenetrans densities in soil in 2004, when the 'Shiraz' vines were eight years old. Pruning weight was reduced by 18.7 to 22.9 % when nematode population densities were greater than 12,000·kg-1 dry soil (average population over 19,000·kg-1 dry soil).
description: VITIS - Journal of Grapevine Research, Vol. 47 No. 3 (2008): Vitis
Molecular Dynamics Simulation Studies of GSK-3β ATP competitive Inhibitors: Understanding the Factors Contributing to Selectivity
Creators: name: Minhajul Arfeen
name: Patel, Rahul
name: Tosif Khan
name: Bharatam, Prasad V.
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Biological Sciences
subject: Ecology
subject: FOS: Biological sciences
subject: Chemistry
subject: Pharmacology
subject: Biochemistry

description: Glycogen Synthase Kinase-3 is a constitutively acting, multifunctional serine threonine kinase, the role of which has been implicated in several physiological pathways and has emerged as a promising target for the treatment of Type-II diabetes and Alzheimer’s disease. In order to provide a detailed understanding of the origin of selectivity determinants of ATP competitive inhibitors, molecular dynamics simulations in combination with MM-PBSA free energy calculations were performed using crystal structures of GSK-3β and CDK-2 in complex with 12 ATP competitive inhibitors. Analyses of energy contributions indicate that electrostatic interaction energy dictates the selectivity of ATP competitive inhibitors against CDK-2. Key interactions as well as residues that potentially make a major contribution to the binding free energy were identified at the ATP binding site. This analysis stresses the need for the inhibitors to interact with Lys85, Thr138 and Arg141 in the binding site of GSK-3β to show selectivity. The residue wise energy decomposition analysis further suggested the additional role of Gln185 in determining the selectivity of maleimides. The results obtained in this study can be utilized to design new selective GSK-3 ATP competitive inhibitors.
Virgichneumon dumeticola
Creators: name: Riedel
name: Pénigot
name: Schwarz
name: Diller
name: Johansson
name: Japoshvili, G.
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hymenoptera
Ichneumonidae
Virgichneumon
Virgichneumon dumeticola

description: Virgichneumon dumeticola (GRAVENHORST, 1829)
M a t e r i a l e x a m i n e d: Trap 2: 1♁ 20.iv.-5.v., 1♁ 5-20.v., 1♁ 20.v.-1.vi., 1♀ 27.vii.- 10.viii.; trap 4: 1♁ 20.v.-1.vi., 2♁♁ 1-15.vi., 1♁ 29.vi.-13.vii., 1♁ 13-27.vii.; trap 7: 1♀ 1♁ 23.iv.- 5.v., 2♀♀ 1♁ 5-20.v., 1♁ 20.v.-1.vi., 1♁ 25-29.vi., 1♀ 2♁♁ 13-27.vii., 1♁ 27.vii.-10.viii., 1♀ 1♁ 10-24.viii., 1♀ 1♁ 21.ix.-5.x.; trap 8: 1♀ 15-29.vi.; trap 12: 1♁ 1-15.vi., 2♁♁ 15-29.vi., 1♁ 29.vi.- 13.vii., 1♁ 27.vii.-10.viii., Trap 12: 1♁ 10-24.viii.; trap 15: 1♁ 2-16.vi.
D i s t r i b u t i o n: Transpalaearctic and Oriental, known from Georgia (RIEDEL et al. 2018).
description: Published as part of Riedel, Pénigot, Schwarz, Diller, Johansson & Japoshvili, G., 2023, Darwin wasps (Hymenoptera, Ichneumonidae) of the Kintrishi National Park, Sakartvelo (Georgia), with descriptions of six new species, pp. 61-153 in Linzer biologische Beiträge 55 (1) on page 107, DOI: 10.5281/zenodo.10788481
Production of trans-resveratrol in 'Cabernet Sauvignon' (Vitis vinifera L.) callus culture in response to ultraviolet-C irradiation
Creators: name: Keskín, N.
name: Kunter, B.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: In this study, the effects of ultraviolet (UV) irradiation time, incubation time and callus age were investigated for the induction of trans-resveratrol production in callus cultures of Vitis vinifera L. 'Cabernet Sauvignon'. Callus tissues were exposed to 254 nm UV light at 10 cm distance from the source for 10 and 15 min by opening covers of the petri dishes in sterile cabin. High Pressure Liquid Chromatography (HPLC) was used for the determination of trans-resveratrol production and concentrations were recorded at 0, 24, 48 and 72 hours after beginning of incubation. Separation by HPLC was achieved using a C18 column and a gradient elution with acetonitrile and water (from 10 to 85 % acetonitrile). The peak of trans-resveratrol was detected at 330 nm and identified from the retention time (12.5 min) trans-resveratrol standard. Determination coefficient for linearity (R2), Limit of Detections (LOD), Limit of Quantification (LOQ) and relative standard deviation (RSD) values of the method were found as 0.9994, 0.12, 0.35 and 1.9, respectively. The highest trans-resveratrol concentration (62.66 μg·g-1 callus fresh weight) was determined in 48 hours of 12 days-old callus cultures irradiated for 10 minutes. Both 10 min and 15 min UV irradiation periods were found to be effective for the induction of trans-resveratrol production and thus callus cultures could be convenient for trans-resveratrol production.
description: VITIS - Journal of Grapevine Research, Vol. 47 No. 4 (2008): Vitis
Virgichneumon faunus
Creators: name: Riedel
name: Pénigot
name: Schwarz
name: Diller
name: Johansson
name: Japoshvili, G.
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hymenoptera
Ichneumonidae
Virgichneumon
Virgichneumon faunus

description: Virgichneumon faunus (GRAVENHORST, 1829)
Material. Trap 7: 1♁ 1-25.vi.
D i s t r i b u t i o n: Transpalaearctic, known from Krasnodar region (TERESHKIN 2006), new record for Georgia.
description: Published as part of Riedel, Pénigot, Schwarz, Diller, Johansson & Japoshvili, G., 2023, Darwin wasps (Hymenoptera, Ichneumonidae) of the Kintrishi National Park, Sakartvelo (Georgia), with descriptions of six new species, pp. 61-153 in Linzer biologische Beiträge 55 (1) on page 107, DOI: 10.5281/zenodo.10788481
Root dynamics and pattern of 'Riesling' on 5C rootstock using minirhizotrons
Creators: name: Lehnart, R.
name: Michel, H.
name: Löhnertz, O.
name: Linsenmeier, A.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Root length density (RLD) in the years from 1994-1997 was estimated using minirhizotrons. The field experiment was conducted on six 'Riesling' vines in Rheingau (Germany). The majority of root distribution was found in soil depths of 60-100 cm with considerable variations between the plants. Roots dynamics showed a periodicity with one or two maxima, depending on year and vine plant. The first peak of RLD was observed around veraison, the second peak appeared after harvest. The rate of root length death was estimated. In the deeper layer the turnover of roots was 60% of the total RLD every year.
description: VITIS - Journal of Grapevine Research, Vol. 47 No. 4 (2008): Vitis
Heterogeneous Catalyzed Ozonation using Cu-Ni-Co Oxides for Degradation of Dichlorophenol
Creators: name: Jignesh Valand
name: Maddila, Suresh
name: Friedrich, Holger B.
name: Sreekantha B. Jonnalagadda
Publisher: name: Taylor & Francis
Year: 2016
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: Inorganic Chemistry

description: The ozone initiated oxidation of dichlorophenol (DCP) in aqueous medium, catalyzed by various loadings of Cu, Ni and Co oxides supported on γ-Al2O3 was studied, as function of ozonation time and pH. Ternary catalyst materials synthesized by a wet-impregnation were fully characterized. The XRD results showed metal oxides were well incorporated within the alumina phase. Microscope images illustrated that metal oxide particles are dispersed on the surface of the support. Dihydroxyfumaric acid and oxalic acid were, respectively, found to be major and minor oxidation products. The oxidation products were characterized by FT-IR, 1H NMR, and mass spectral data.
Microsatellite genotyping of old Slovenian grapevine varieties (Vitis vinifera L.) of the Primorje (coastal) winegrowing region
Creators: name: Štajner, N.
name: Korošek-Koruza, Z.
name: Rusian, D.
name: Javornik, B.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Thirty-three grapevine varieties from Slovenia were genotyped at 21 microsatellite loci in order accurately to identify varieties and to evaluate their synonyms and homonyms, including varieties cultivated in neighbouring countries. Among Slovenian varieties some previously assumed synonyms were confirmed and some new ones were discovered: ‘Poljšakica Drnovk’ = ‘Istrska Malvazija’, ‘Pikolit Italy’ = ‘Pikolit Vienna’, ‘Vitovska grganja’ = ‘Racuk’ and ‘Prosecco’ = ‘Glera’ = ‘Števerjana’. Types of Zelen (‘Zelen Pokov’, ‘Zelen 66’ and ‘Zelen 2.4’) were distinct at several microsatellite loci so can only be considered to be homonyms. Two ‘Picolit’ types were considered to be ‘true-to-type’ on the basis of comparison with ‘Picolit’ clones from Italy. Synonymy between ‘Heunisch’ and ‘Belina’ was not confirmed in our study, since ‘Belina Pleterje’ differentiated from ‘Heunisch weiss’ at 13 out of 19 loci. Comparison of ‘Vitovska grganja’ from Slovenia with ‘Vitouska’ from Italy also showed dissimilarities at the majority of the analysed loci.
description: VITIS - Journal of Grapevine Research, Vol. 47 No. 4 (2008): Vitis
Wave crest and trough distributions in a broad-banded directional wave field
Creators: name: Toffoli, Alessandro
name: Bitner-Gregersen, E. M.
name: Onorato, M.
name: Babanin, Alexander
Publisher: name: Swinburne
Year: 2024

description: It is well established that the modulational instability enhances the probability of occurrence for extreme events in long crested wave fields. Recent studies, however, have shown that the coexistence of directional wave components can reduce the effects related to the modulational instability. Here, numerical simulations of the Euler equations are used to investigate whether the modulational instability may produce significant deviations from second-order statistical properties of surface gravity waves when short crestness (i.e., directionality) is accounted for. The case of a broad-banded directional wave field (i.e. wind sea) is investigated. The analysis is concentrated on the wave crest and trough distribution. For completeness a comparison with a unidirectional wave field is presented also. Results will show that the distributions based on second-order theory provide a good estimate for the simulated crest and trough height also at low probability levels.
DAILY LIFE OF CITY DWELLERS IN KARAKALPAKSTAN DURING  THE WORLD WAR II THE WORLD WAR II
Creators: name: Allamuratov, Gulmurat
Publisher: name: Zenodo
Year: 2024

description: This article is about the issues of everyday life of the urban population of Karakalpakstan in the period of beginning of World War II. Particular attention is paid to the living conditions of the residents of the city of Nukus - the capital of the republic, where on the eve of the war the republican management and management bodies moved. The construction of the city continued, which led to the rapid growth of the city's population on the eve of the war. An analysis of archival documents and materials shows that during the Second World War, there were serious changes in the daily life of all segments of the population. It is indicated that the beginning of the Second World War brought new rules of life and work to the life of the townspeople. Labor discipline has intensified, there have been changes in the provision of food to the cities, and the time for rest and leisure of citizens has been reduced. The harsh everyday life of the war has influenced both the consciousness of people and their patriotic mood. Because of the difficult military situation, there was a reduction in food and industrial state funds, which affected the provision of citizens with goods of mass demand.
Structure of the Texas Orocline beneath the sedimentary cover (southeast Queensland, Australia)
Creators: name: S. Brooke-Barnett
name: G. Rosenbaum
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Biological Sciences
subject: Ecology
subject: FOS: Biological sciences
subject: Earth and Environmental Sciences

description: The New England Orogen in eastern Australia is characterised by orogenic-scale curvatures (oroclines). The largest and most prominent curvature in this system is the Texas Orocline, but its subsurface geometry is still poorly constrained. A large component of the orocline is covered by post-oroclinal sedimentary rocks, which obscure deeper sections of the orocline and make it difficult to understand how the structure is connected to other segments of the New England Orogen. Here, we present geophysical data that elucidate the structure of the Texas Orocline below the sedimentary cover. Using 2D seismic, aeromagnetic TMI (total magnetic intensity) and Bouguer gravity datasets, in combination with outcrop and well data, we identified the depth to the New England ‘basement’ and significant faults intersecting it. We also traced the strongly contorted subsurface continuation of the Peel-Yarrol Fault System, which is characterised by local gravity and magnetic anomalies associated with isolated serpentinite outcrops. Constraints on the timing of oroclinal bending were obtained from the interpretation of seismic transects, which showed that early Permian sedimentary rocks of the Bowen Basin were deposited in a subtrough that deviates from the general north–south trend of the Bowen Basin. The subtrough is oriented approximately parallel to the western limb of the Texas Orocline, thus suggesting that the orocline formed during and/or after early Permian rifting. Our analysis indicates that initial bending occurred contemporaneously with the development of the early Permian rift basins, most likely in the backarc region of a retreating subduction zone. Subsequently, phases of strike-slip and contractional deformation have further tightened the pre-existing curvatures.
Virgichneumon krapinensis
Creators: name: Riedel
name: Pénigot
name: Schwarz
name: Diller
name: Johansson
name: Japoshvili, G.
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hymenoptera
Ichneumonidae
Virgichneumon
Virgichneumon krapinensis

description: Virgichneumon krapinensis (SCHMIEDEKNECHT, 1928)
M a t e r i a l e x a m i n e d: Trap 7: 1♁ 5-20.v.; trap 8: 1♀ 15-29.vi.
D i s t r i b u t i o n: Western Palaearctic, known from the Caucasus region (RASNITSYN & SIYTAN 1981), new record for Georgia.
description: Published as part of Riedel, Pénigot, Schwarz, Diller, Johansson & Japoshvili, G., 2023, Darwin wasps (Hymenoptera, Ichneumonidae) of the Kintrishi National Park, Sakartvelo (Georgia), with descriptions of six new species, pp. 61-153 in Linzer biologische Beiträge 55 (1) on page 107, DOI: 10.5281/zenodo.10788481
‘Malvasia nera di Brindisi/Lecce’ grapevine cultivar (Vitis vinifera L.) originated from ‘Negroamaro’ and ‘Malvasia bianca lunga’
Creators: name: Crespan, M.
name: Coletta, A.
name: Crupi, P.
name: Giannetto, S.
name: Antonacci, D.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: ‘Malvasia nera di Brindisi’ and ‘Malvasia nera di Lecce’ are two of the few Malvasias with black berries and belong to the Apulian ampelographic assortment (South Italy). Their presumed synonymy has been recently ascertained with SSR markers and therefore these two black 'Malvasias' can be considered as an unique variety. We discovered that this cultivar is the cross between ‘Malvasia bianca lunga’ alias ‘Malvasia del Chianti’ and ‘Negroamaro’ by using 42 nuclear SSR. Both parents belong to the Apulian varietal resources, since centuries. So far, ‘Malvasia nera di Brindisi/Lecce’ origin has been obscure; now we may assert that this cultivar was born right in Apulia. Three sets of chloroplast SSR loci were used to determine the female and the male parent: 6 ccmp loci, already used in previous pedigree studies, 15 ccSSR loci and 2 NTCP loci, derived from tobacco. The second set of loci was sequenced in order to compare the length of the markers with the reference species where they were originally obtained: in 4 cases no microsatellite motives were detected and in other 4 cases the perfect repetition found in tobacco was not maintained in grape. Unfortunately, the three sets of markers failed to show any polymorphism. A detailed comparison of the black Malvasia morphology with its two parents showed a closer similarity to ‘Negroamaro’. Also the anthocyanin profile is in agreement with that of the black parent; its varietal aroma presents interesting levels of free and bound 2-phenylethanol, responsible for rose flavor, and of bound linalool compounds.
description: VITIS - Journal of Grapevine Research, Vol. 47 No. 4 (2008): Vitis
PKCι depletion initiates mitotic slippage-induced senescence in glioblastoma
Creators: name: Restall, Ian J
name: Parolin, Doris A E
name: Daneshmand, Manijeh
name: Hanson, Jennifer E L
name: Simard, Manon A
name: Fitzpatrick, Megan E
name: Ritesh Kumar
name: Lavictoire, Sylvie J
name: Lorimer, Ian A J
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Biophysics
subject: 29999 Physical Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Physiology
subject: Immunology
subject: FOS: Clinical medicine
subject: Developmental Biology
subject: Cancer
subject: Hematology

description: Cellular senescence is a tumor suppressor mechanism where cells enter a permanent growth arrest following cellular stress. Oncogene-induced senescence (OIS) is induced in non-malignant cells following the expression of an oncogene or inactivation of a tumor suppressor. Previously, we have shown that protein kinase C iota (PKCι) depletion induces cellular senescence in glioblastoma cells in the absence of a detectable DNA damage response. Here we demonstrate that senescent glioblastoma cells exhibit an aberrant centrosome morphology. This was observed in basal levels of senescence, in p21-induced senescence, and in PKCι depletion-induced senescence. In addition, senescent glioblastoma cells are polyploid, Ki-67 negative and arrest at the G1/S checkpoint, as determined by expression of cell cycle regulatory proteins. These markers are all consistent with cells that have undergone mitotic slippage. Failure of the spindle assembly checkpoint to function properly can lead to mitotic slippage, resulting in the premature exit of mitotic cells into the G1 phase of the cell cycle. Although in G1, these cells have the replicated DNA and centrosomal phenotype of a cell that has entered mitosis and failed to divide. Overall, we demonstrate that PKCι depletion initiates mitotic slippage-induced senescence in glioblastoma cells. To our knowledge, this is the first evidence of markers of mitotic slippage directly in senescent cells by co-staining for senescence-associated β-galactosidase and immunofluorescence markers in the same cell population. We suggest that markers of mitotic slippage be assessed in future studies of senescence to determine the extent of mitotic slippage in the induction of cellular senescence.
Relatedness of resistance to anthracnose and to white rot in Chinese wild grapes
Creators: name: Li, Dan
name: Wan, Yizhen
name: Wang, Yuejin
name: He, Puchao
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Resistance to anthracnose and to white rot in 56 accessions of 13 Chinese wild Vitis species, V. amurensis, V. quinquangularis, V. romanetii, V. adstricta, V. pseudoreticulata, V. piazezkii, V. davidii, V. davidii var. cyanocarpa, V. liubanensis, V. qinlingensis, V. bashanica, V. yeshanensis and V. hankockii was evaluated under natural conditions in 1996-1998. All 56 accessions and the 13 species showed resistance to anthracnose. All nine accessions of V. quinquangularis, and one accession each of V. romanetii, V. adstricta and V. pseudoreticulata exhibited susceptibility to white rot; the remaining 44 accessions (78.57 %) and the other 9 species presented resistant to grape white rot. Two V. vinifera cultivars (‘Cabernet Sauvignon’ and ‘Chardonnay’) were susceptible to anthracnose and highly susceptible to white rot. The r-value of susceptibility indices between anthracnose and white rot in the 56 accessions is insignificant. In Chinese wild grapes, the mechanisms for producing resistance to both diseases would be very different, but inheritance of resistance to these two diseases in a certain cross is somewhat related by molecular data.
description: VITIS - Journal of Grapevine Research, Vol. 47 No. 4 (2008): Vitis
The effect of third-order nonlinearity on statistical properties of random directional waves in finite depth
Creators: name: Toffoli, Alessandro
name: Benoit, M.
name: Onorato, M.
name: Bitner-Gregersen, E. M.
Publisher: name: Swinburne
Year: 2024

description: It is well established that third-order nonlinearity produces a strong deviation from Gaussian statistics in water of infinite depth, provided the wave field is long crested, narrow banded and sufficiently steep. A reduction of third-order effects is however expected when the wave energy is distributed on a wide range of directions. In water of arbitrary depth, on the other hand, third-order effects tend to be suppressed by finite depth effects if waves are long crested. Numerical simulations of the truncated potential Euler equations are here used to address the combined effect of directionality and finite depth on the statistical properties of surface gravity waves; only relative water depth kh greater than 0.8 are here considered. Results show that random directional wave fields in intermediate water depths, kh=O(1), weakly deviate from Gaussian statistics independently of the degree of directional spreading of the wave energy.
Synergy in Spatial Models of Voting: How Critical Cases Show That Proximity, Direction and Discounting Are Friends, Not Foes
Creators: name: Weber, Till
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Science Policy
subject: Cancer
subject: Biological Sciences
subject: Sociology
subject: FOS: Sociology
subject: Ecology
subject: FOS: Biological sciences
subject: Medicine

description: The spatial theory of elections is widely used to examine how party policy is linked to voter preferences. Three classic models – proximity, direction and discounting – lay claim to the nature of this link, but their predictions of voting behavior are almost identical. I resolve the problem by decomposing 28 multi-party systems into about 1 million party triplets. This allows me to isolate “critical cases” where model predictions diverge. The combinatorial approach reveals a degree of collinearity between models that may surprise even longtime experts in the field. But while collinearity is a nuisance for scholars, it is a blessing for voters. I find disproportionately strong policy voting when all predictions agree, indicating that there is synergy between the three spatial logics. A selection effect can be excluded using propensity-score matching. These findings suggest that concurrence of proximity, directional and discounting elements in the vote function is inherent to electoral competition.
Virgichneumon monostagon
Creators: name: Riedel
name: Pénigot
name: Schwarz
name: Diller
name: Johansson
name: Japoshvili, G.
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hymenoptera
Ichneumonidae
Virgichneumon
Virgichneumon monostagon

description: Virgichneumon monostagon (GRAVENHORST, 1820)
M a t e r i a l e x a m i n e d: Trap7:1♁25-29.vi.
D i s t r i b u t i o n: Transpalaearctic, known from Azerbaijan (ALIYEV 1999), new record for Georgia.
description: Published as part of Riedel, Pénigot, Schwarz, Diller, Johansson & Japoshvili, G., 2023, Darwin wasps (Hymenoptera, Ichneumonidae) of the Kintrishi National Park, Sakartvelo (Georgia), with descriptions of six new species, pp. 61-153 in Linzer biologische Beiträge 55 (1) on page 107, DOI: 10.5281/zenodo.10788481
New PCR primers applied to characterize distribution of Botrytis cinerea populations in French vineyards
Creators: name: Martinez, F.
name: Corio-Costet, M. F.
name: Levis, C.
name: Coarer, M.
name: Fermaud, M.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: The phytopathogenic fungus Botrytis cinerea is a complex of two main genetic groups, Group-I and Group-II, the latter including different TE types distinguished by the presence or absence of two transposable elements (TE), Boty and Flipper. In populations from Bordeaux vineyards (n = 470), the frequency distribution into these genetic subdivisions showed that Group-I isolates were rare (2.3 %) whereas, within Group-II, four TE types were identified by dot blot in very different proportions: II-transposa (59.8 %), II-boty (21.3 %), II-vacuma (15.5 %) and II-flipper (1.1 %). To distinguish the TE types by PCR, a first primer pair was designed within the Flipper sequence which yielded a not-expected 2287 bp fragment. The 5’ extremity of this fragment was sequenced revealing a potential genomic insertion site of the Flipper element allowing the design of a new overlapping PCR primer. Detection of the Flipper element by two newly developed PCR tests and a published one (F300-F1550) was consistent with dot blot results in Group-I and II-transposa (concordance rates from 94.6 % to 100 %). However, discrepancies between PCR and dot blot were noticeable especially in II-boty, but also in II-vacuma (concordance rates from 33.3 % to 38.0 % and from 62 % to 81.2 %, respectively). On the basis of TE-type identification strengthened by combining different PCR and dot blot results, the spatiotemporal distribution of the Group-II isolates was assessed according to the developmental stage and the host organ of grapevine. In Bordeaux as in Loire valley vineyards, similar distribution patterns were described showing significant differences between the most sampled TE types. The II-transposa isolates predominated on grape berries at the end of the season, whereas the II-vacuma ones were detected mostly at flowering. Lastly, the II-boty isolates were more often detected on grapevine leaves than on flowers or berries.
description: VITIS - Journal of Grapevine Research, Vol. 47 No. 4 (2008): Vitis