Additional file 1 of Differential expressions of anthocyanin synthesis genes underlie flower color divergence in a sympatric Rhododendron sanguineum complex
Creators: name: Ye, Lin-Jiang
name: Mӧller, Michael
name: Luo, Ya-Huang
name: Zou, Jia-Yun
name: Zheng, Wei
name: Wang, Yue-Hua
name: Liu, Jie
name: Zhu, An-Dan
name: Hu, Jin-Yong
name: Li, De-Zhu
name: Gao, Lian-Ming
Publisher: name: figshare
Year: 2021
Subjects: subject: Genetics
subject: FOS: Biological sciences

description: Additional file 1: Table S1. Statistics of the sequencing, assembly, and filtering of transcriptomes of samples of three varieties of the Rhododendron sanguineum complex. Table S2. Basic information of the transcriptome sequencing and alignment rates of 18 RNA libraries of two tissues of three varieties of the Rhododendron sanguineum complex. Table S3. BUSCO statistics for transcriptome assembly quality assessment of three varieties of the Rhododendron sanguineum complex. Table S4. Orthologous cluster statistics across three varieties of the Rhododendron sanguineum complex. Table S5. Identification and annotation of orthologous clusters across three varieties of the Rhododendron sanguineum complex. Table S6. List of the 40 predicted anthocyanin-associated genes and paralogs found in the Rhododendron sanguineum complex. Table S7. Primer information used for qRT-PCR validation of 12 gene paralogs involved in anthocyanin synthesis in three varieties of the Rhododendron sanguineum complex. Table S8. Statistical information of promoters for all genes among the Rhododendron sanguineum complex. Figure S1. BUSCO quality assessment results of transcriptome assemblies for three varieties of the Rhododendron sanguineum complex (a) and annotation of 15,164 orthologous cluster hits against six different databases (b). RsS – R. sanguineum var. sanguineum; RsH – R. sanguineum var. haemaleum; RsD – R. sanguineum var. didymoides. Figure S2. Annotation of the 15,164 one to one single copy orthologs among three varieties of the Rhododendron sanguineum complex through interrogation against three different databases. Figure S3. Phylogenetic and genetic clustering results for the three varieties of the Rhododendron sanguineum complex. Figure S4. Gene expression patterns of all 18 samples of three varieties of the Rhododendron sanguineum complex. Figure S5. Volcano plots of differentially expressed genes (DEGs) based on pairwise comparisons of the three varieties of the Rhododendron sanguineum complex. Figure S6. Heatmap and results of the functional enrichment of differentially expressed genes (DEGs) among the three varieties of the Rhododendron sanguineum complex. Figure S7. Hierarchical clustering of normalized expression levels of 23 candidate genes show distinct gene expression profiles in comparison of RsH and RsD. Variety code as in Figure S1. Red indicates high expression, and blue indicates low expression. Figure S8. Hierarchical clustering of normalized expression levels of 25 candidate genes show distinct gene expression profiles in comparison of RsH and RsS. Variety code as in Figure S1. Red represents high expression, and blue represents low expression. Figure S9. Hierarchical clustering of normalized expression levels of 12 candidate genes show distinct gene expression profiles in comparison of RsS and RsD. Variety code as in Figure S1. Red represents high expression, and blue represents low expression. Figure S10. A common layout of the promoter architecture of the 13 anthocyanin genes across varieties. Variety code as in Figure S1.