title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐ¾ÑÑÐ°Ð²Ð° Ð²Ð°ÐºÑÐ¸Ð¾Ð½Ð½ÑÑ Ð²Ð¸ÑÑÑÐ¾Ð² Ð³ÑÐ¸Ð¿Ð¿Ð° A/Victoria/361/2011 (H3N2) Ð½Ð° Ð¸Ñ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ñ Ð² ÐºÑÐ»ÑÑÑÑÐµ ÐºÐ»ÐµÑÐ¾Ðº MDCK Ð¸ ÐºÑÑÐ¸Ð½ÑÑ ÑÐ¼Ð±ÑÐ¸Ð¾Ð½Ð°Ñ
title: Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÑÐºÐ°Ñ ÑÐ°Ð±Ð¾ÑÐ°
titleType: Subtitle
Creators: name: ÐÐ½Ð´ÑÐµÐµÐ²Ð°, ÐÐ²Ð³ÐµÐ½Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2016
Subjects: Ð²Ð¸ÑÑÑ Ð³ÑÐ¸Ð¿Ð¿Ð° Ð
Ð²Ð°ÐºÑÐ¸Ð½Ð°
ÑÐµÐ°ÑÑÐ¾ÑÑÐ°Ð½Ñ
ÑÐ¾ÑÑÐ°Ð² Ð³ÐµÐ½Ð¾Ð¼Ð°
ÐºÑÐ»ÑÑÑÑÐ° ÐºÐ»ÐµÑÐ¾Ðº MDCK
influenza A virus
vaccine
reassortant
genome composition
MDCK cell culture

description: Ð¦ÐµÐ»ÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¾ ÑÑÐ°Ð²Ð½Ð¸ÑÑ Ð²ÑÑÐ¾ÐºÐ¾ÑÐµÐ¿ÑÐ¾Ð´ÑÐºÑÐ¸Ð²Ð½ÑÐµ ÑÐµÐ°ÑÑÐ¾ÑÑÐ°Ð½ÑÑ, Ð¿Ð¾Ð´Ð¾Ð±Ð½ÑÐµ Ð²Ð¸ÑÑÑÑ Ð³ÑÐ¸Ð¿Ð¿Ð° A/Victoria/361/2011, RA-31 Ð¸ RA-32 (ÐÐÐ Ð³ÑÐ¸Ð¿Ð¿Ð°, Ð Ð¾ÑÑÐ¸Ñ) Ñ ÑÐµÐ°ÑÑÐ¾ÑÑÐ°Ð½ÑÐ°Ð¼Ð¸ NIB-79 (NIBSC, ÐÐµÐ»Ð¸ÐºÐ¾Ð±ÑÐ¸ÑÐ°Ð½Ð¸Ñ) Ð¸ IVR-165 (CSL, ÐÐ²ÑÑÑÐ°Ð»Ð¸Ñ), ÑÐµÐºÐ¾Ð¼ÐµÐ½Ð´Ð¾Ð²Ð°Ð½Ð½ÑÐ¼Ð¸ ÐÐÐ Ð´Ð»Ñ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´ÑÑÐ²Ð° ÐºÐ¾Ð¼Ð¿Ð¾Ð½ÐµÐ½ÑÐ° Ð(H3N2) Ð¿ÑÐ¾ÑÐ¸Ð²Ð¾Ð³ÑÐ¸Ð¿Ð¿Ð¾Ð·Ð½Ð¾Ð¹ Ð²Ð°ÐºÑÐ¸Ð½Ñ. ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð²ÐºÐ»ÑÑÐ°Ð»Ð¾ Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐ¸ ÑÐ¾ÑÑÐ° ÑÐµÐ°ÑÑÐ¾ÑÑÐ°Ð½ÑÐ¾Ð² Ð² ÐºÑÑÐ¸Ð½ÑÑ ÑÐ¼Ð±ÑÐ¸Ð¾Ð½Ð°Ñ Ð¸ ÐºÐ»ÐµÑÐºÐ°Ñ MDCK, Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð¾ÑÐ½Ð¾ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð° Ð³ÐµÐ¼Ð°Ð³Ð³Ð»ÑÑÐ¸Ð½Ð¸Ð½Ð° (ÐÐ) Ð¸ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾-Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð°Ð½Ð°Ð»Ð¸Ð·. ÐÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð½Ð°Ð»Ð¸ÑÐ¸Ðµ PB1 Ð³ÐµÐ½Ð° ÑÐ¿Ð¸Ð´ÐµÐ¼Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð²Ð¸ÑÑÑÐ° Ð² ÑÐ¾ÑÑÐ°Ð²Ðµ Ð³ÐµÐ½Ð¾Ð¼Ð° ÑÐµÐ°ÑÑÐ¾ÑÑÐ°Ð½ÑÐ° ÑÐ½Ð¸Ð¶Ð°Ð»Ð¾ ÑÐºÐ¾ÑÐ¾ÑÑÑ ÐµÐ³Ð¾ ÑÐµÐ¿ÑÐ¾Ð´ÑÐºÑÐ¸Ð¸ Ð² ÐºÐ»ÐµÑÐºÐ°Ñ MDCK. ÐÐ¸Ð½Ð°Ð¼Ð¸ÐºÐ° ÑÐµÐ¿ÑÐ¾Ð´ÑÐºÑÐ¸Ð¸ Ð² ÐºÑÑÐ¸Ð½ÑÑ ÑÐ¼Ð±ÑÐ¸Ð¾Ð½Ð°Ñ Ð±ÑÐ»Ð° ÑÑÐ¾Ð¶Ð° Ñ Ð²ÑÐµÑ ÑÐµÐ°ÑÑÐ¾ÑÑÐ°Ð½ÑÐ¾Ð², Ð¿ÑÐ¸ ÑÑÐ¾Ð¼ Ð¾ÑÐ½Ð¾ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ðµ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð¾ HA Ð±ÑÐ»Ð¾ ÑÐ°Ð·Ð½ÑÐ¼ (Ð½Ð°Ð¸Ð±Ð¾Ð»ÑÑÐµÐµ Ð·Ð½Ð°ÑÐµÐ½Ð¸Ðµ Ð¾ÑÐ¼ÐµÑÐµÐ½Ð¾ Ñ ÑÑÐ°Ð¼Ð¼Ð° RA-31). Ð¡ÐµÐºÐ²ÐµÐ½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð³ÐµÐ½Ð¾Ð¼Ð° Ð²Ð¸ÑÑÑÐ¾Ð² Ð¿Ð¾ÐºÐ°Ð·Ð°Ð»Ð¾ Ð½Ð°Ð»Ð¸ÑÐ¸Ðµ Ð°Ð¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾ÑÐ½ÑÑ Ð·Ð°Ð¼ÐµÐ½ Ð² Ð¼Ð°ÑÑÐ¸ÐºÑÐ½Ð¾Ð¼ Ð±ÐµÐ»ÐºÐµ M2 Ð¸ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð½Ð¾Ð¼ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐµ, ÑÑÐ¾ Ð¼Ð¾Ð¶ÐµÑ Ð¾Ð±ÑÑÑÐ½Ð¸ÑÑ ÑÐ°Ð·Ð½Ð¸ÑÑ Ð² ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ð¸ Ð¸Ð¼Ð¼ÑÐ½Ð¾ÐºÐ¾Ð¼Ð¿ÐµÑÐµÐ½ÑÐ½Ð¾Ð³Ð¾ HA.â
description: The aim of the study was to compare Ð/Victoria/361/2011(H3N2)-like high growth reassortant influenza viruses RA31, RA32 (Research Institute of Influenza, Russia) with NIB79 (NIBSC, UK) and IVR165 (CSL, Australia), recommended by WHO as A(H3N2) vaccine component.The study included research of the viral growth dynamics in chicken embryos or MDCK cells, assessment of HA antigen content and molecular genetic analysis.It was shown, that introduction of PB1 gene of epidemic virus into the reassortant genome reduced the rate of its reproduction in MDCK cell culture. However, the dynamics of reproduction in chicken embryos was similar for all reassortants, while the relative amount of the HA protein was variable (RA-31 reassortant yielded the highest HA content). Viral genome sequencing showed the presence of amino acid differences in polymerase and matrix protein M2, which may explain the difference in the relative content of immunocompetent HA.