Metagenomic reconstructions of caecal microbiome in Landes, Roman and Zhedong White geese
Creators: name: Zhu, J.
name: Song, Y.
name: Xiao, Y.
name: Ma, L.
name: Hu, C.
name: Yang, H.
name: Wang, X.
name: Lyu, W.
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Microbiology
subject: FOS: Biological sciences
subject: Genetics
subject: Environmental Sciences not elsewhere classified
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: Biological Sciences not elsewhere classified
subject: Inorganic Chemistry
subject: FOS: Chemical sciences

description: The caecal microbiota in geese plays a crucial role in determining the host’s health, disease status and behaviour, as evidenced by extensive epidemiological data. Despite this, there is a dearth of information concerning the gut metagenome of geese.The present investigation conducted 10× metagenomic sequencing of caecal content samples obtained from three distinct goose species, namely Landes geese, Roman geese and Zhedong White geese (<i>n</i> = 5), to explore the contribution of the gut microbiome to carbohydrate metabolism. In total, 337GB of Illumina data was generated, which identifiedf 1,048,575 complete genes and construction of 331 metagenomic bins, encompassing 78 species from nine phyla.The genes were allocated to 375 pathways using the Kyoto Encyclopedia of Genes and Genome (KEGG) analysis. The most abundant classes in the caecum of geese were confirmed to be glycoside hydrolases (GHs), glycosyl transferases (GTs), as identified through the carbohydrate-active enzyme (CAZyme) database mapping. <i>Subdoligranulum variabile</i> and <i>Mediterraneibacter glycyrrhizinilyticus</i> were discovered to potentially facilitate carbohydrate digestion in geese.Notwithstanding, further investigation and validation are required to establish a connection between these species and CAZymes. Based on binning analysis, <i>Mediterraneibacter glycyrrhizinilyticus</i> and <i>Ruminococcus sp. CAG:177</i> are potential species in LD geese that contribute to the production of fatty liver. The caecal microbiota in geese plays a crucial role in determining the host’s health, disease status and behaviour, as evidenced by extensive epidemiological data. Despite this, there is a dearth of information concerning the gut metagenome of geese. The present investigation conducted 10× metagenomic sequencing of caecal content samples obtained from three distinct goose species, namely Landes geese, Roman geese and Zhedong White geese (<i>n</i> = 5), to explore the contribution of the gut microbiome to carbohydrate metabolism. In total, 337GB of Illumina data was generated, which identifiedf 1,048,575 complete genes and construction of 331 metagenomic bins, encompassing 78 species from nine phyla. The genes were allocated to 375 pathways using the Kyoto Encyclopedia of Genes and Genome (KEGG) analysis. The most abundant classes in the caecum of geese were confirmed to be glycoside hydrolases (GHs), glycosyl transferases (GTs), as identified through the carbohydrate-active enzyme (CAZyme) database mapping. <i>Subdoligranulum variabile</i> and <i>Mediterraneibacter glycyrrhizinilyticus</i> were discovered to potentially facilitate carbohydrate digestion in geese. Notwithstanding, further investigation and validation are required to establish a connection between these species and CAZymes. Based on binning analysis, <i>Mediterraneibacter glycyrrhizinilyticus</i> and <i>Ruminococcus sp. CAG:177</i> are potential species in LD geese that contribute to the production of fatty liver.