Συσχέτιση της λοίμωξης από τον ιό των ανθρώπινων θηλωμάτων (HPV) στο στοματικό βλεννογόνο και τον τράχηλο της μήτρας σεξουαλικά ενεργών γυναικών
Creators: name: Παρχαρίδης, Ευάγγελος Θεόδωρος
Publisher: name: Aristotle University of Thessaloniki
Year: 2020
Subjects: HPV-ιοί
HPV-λοίμωξη
Kαρκίνος στόματος
HPV
HPV-DNA test
Swabs

description: Εισαγωγή: Οι HPV-ιοί είναι ομάδα DNA-ιών που ενοχοποιούνται για πληθώρα νοσολογικών οντοτήτων. Η υψηλού κινδύνου HPV λοίμωξη έχει συσχετισθεί με την καρκινογένεση στον τράχηλο της μήτρας και σε μικρότερο βαθμό στο στοματοφάρυγγα. Σκοπός της έρευνας είναι η μελέτη της πιθανής συσχέτισης της HPV-λοίμωξης μεταξύ των δύο ανατομικών περιοχών. Υλικά &amp; Μεθοδολογία: Στο δείγμα εντάχθηκαν 121 σεξουαλικά ενεργές γυναίκες με παθολογία τραχήλου της μήτρας, σχετιζόμενη με HPV-λοίμωξη διαγνωσμένη αρχικά με κολποσκόπηση, στη συνέχεια με τεστ-Παπ και με επιβεβαίωση με HPV-DNA τεστ που δεν έχουν εμβολιασθεί για τον HPV. Αποτελέσματα: Στο 3,33% του δείγματος ανιχνεύτηκε HPV στη στοματική κοιλότητα με HPV-DNA τεστ με το 75% αυτών να τυποποιείται ο υψηλού-κινδύνου 16 ενώ σε κανένα περιστατικό δεν εντοπίστηκε κλινικά αλλοίωση HPV-σχετιζόμενη. Συμπεράσματα: Ο επιπολασμός εμφανίζει μεγάλη διακύμανση μεταξύ των διαφόρων ερευνητών, από 3,33% ως 81%. Είναι σταθερή όμως η ανίχνευση τύπων υψηλού-κινδύνου και αυτό πρέπει να οδηγήσει σε νέες πιο στοχευμένες έρευνες.
description: Human papillomavirus is implicated in causation of cervical cancer and is considered a necessary factor for this type of cancer. HPV is also detected in other anatomical sites like the oral cavity and is associated to oropharyngeal cancer. OBJECTIVE. To inquire into the cervical and oral HPV detection rates among women referred to colposcopy for abnormal CaCx-screening results as well as to assess the concordance of different HPV types among these anatomical sites, we conducted the presented analysis on women who have already a cervical HPV infection, transient or productive. METHODS. The presented analysis was conducted on samples collected from women referred to the Second Department of Obstetrics and Gynecology, Hippokration Hospital, Medical Faculty Aristotle University of Thessaloniki, Greece. Women recruited, aged 21-65 years old, referred to colposcopy. Exclusion criteria had been pregnancy, prior total hysterectomy due to various reasons, prior chemo-radiation treatment and vaccinated women as well. In referred women cervical smear was taken as well as sample from the oral cavity. Routine colposcopy involved multiple biopsies and/or ECC. The oral sample obtained as an oral rinse specimen was collected using 15mL of alcohol solution for 30 seconds in sterile vials. HPV detection was performed by PCR genotyping (CLART2,Genomica). Descriptive statistics were used for HPV-detection rates for the determination of HPV positivity concordance between the anatomical sites. Oral clinical examination was conducted. All mucosal surfaces were examined and lesions were recorded on a diagram. RESULTS. Out of 121 referred women, high-risk HPV-DNA was detected in 53.7% and 3.33% at cervix and oral cavity respectively. The only hr-HPV types detected were HPV16 and HPV53. No pathological lesion was detected on the oral cavities. CONCLUSIONS. In recent years there has been an increasing interest in HPV infection on anatomical areas other than the cervix; HPV is detected in 99.7% of all cervical cancer cases worldwide. HPV viruses can cause a wide range of lesions in the oral cavity, benign, potentially malignant and malignant. Prevalence varies widely among researchers from 11.6% to 81%. In our survey, it was detected in 3.33% of women. Significant differences in outcomes may be related to patients' sexual habits, temperament and lifestyle, but may also be sensitive to methodology and detection methods. However, the constant detection of high or potentially high-risk types in any non-prevalent research should lead to a new research possibly related to prophylactic vaccines in order to reduce the spread of HPV virus that causes life-threatening lesions and raises public health and health insurance costs. Key questions are whether saliva-based HPV tests can determine the risk of possible carcinogenicity and whether they can be a prognosis or management guide for already installed HPV-associated cancer in patients and whether prophylactic vaccination will actually be effective in oropharynx to be dramatically reduced in the future generations or even eliminate HPV-related lesions.