title: Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° ÑÐµÑÑ-ÑÐ¸ÑÑÐµÐ¼Ñ Ð´Ð»Ñ Ð¾ÑÐµÐ½ÐºÐ¸ Ð¼Ð¸ÐºÑÐ¾ÑÐ°ÑÐµÐ»Ð»Ð¸ÑÐ½Ð¾Ð¹ Ð½ÐµÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾ÑÑÐ¸ Ð² Ð¾Ð±ÑÐ°Ð·ÑÐ°Ñ ÑÐ°ÐºÐ° ÑÐµÐ»Ð° Ð¼Ð°ÑÐºÐ¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Development of a test system for assessing microsatellite instability in uterine body cancer samples
Creators: name: ÐÑÐ±Ð½Ð¾Ð², ÐÐ¸ÑÐ°Ð¸Ð»
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÑÐ°Ðº ÑÐµÐ»Ð° Ð¼Ð°ÑÐºÐ¸
Ð¸Ð¼Ð¼ÑÐ½Ð¾ÑÐµÑÐ°Ð¿Ð¸Ñ
ÐºÐ°Ð¿Ð¸Ð»Ð»ÑÑÐ½ÑÐ¹ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÐ·
Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð½Ð°Ñ ÑÐµÐ¿Ð½Ð°Ñ ÑÐµÐ°ÐºÑÐ¸Ñ
Ð¼Ð¸ÐºÑÐ¾ÑÐ°ÑÐµÐ»Ð»Ð¸ÑÐ½Ð°Ñ Ð½ÐµÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾ÑÑÑ
uterine body cancer
immunotherapy
capillary electrophoresis
polymerase chain reaction
microsatellite instability

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° ÑÐµÑÑâÑÐ¸ÑÑÐµÐ¼Ñ Ð´Ð»Ñ Ð¾ÑÐµÐ½ÐºÐ¸ Ð¼Ð¸ÐºÑÐ¾ÑÐ°ÑÐµÐ»Ð»Ð¸ÑÐ½Ð¾Ð¹ Ð½ÐµÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾ÑÑÐ¸ Ð² Ð¾Ð±ÑÐ°Ð·ÑÐ°Ñ ÑÐ°ÐºÐ° ÑÐµÐ»Ð° Ð¼Ð°ÑÐºÐ¸Â».Â ÐÐ¸ÐºÑÐ¾ÑÐ°ÑÐµÐ»Ð»Ð¸ÑÐ½Ð°Ñ Ð½ÐµÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾ÑÑÑ (MSI) â ÑÐ°ÑÐ¿ÑÐ¾ÑÑÑÐ°Ð½ÐµÐ½Ð½Ð¾Ðµ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ Ð² Ð³ÐµÐ½Ð¾ÑÐ¸Ð¿Ðµ Ð¾Ð¿ÑÑÐ¾Ð»ÐµÐ²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐ°ÐºÐ° ÑÐµÐ»Ð° Ð¼Ð°ÑÐºÐ¸. ÐÐ½Ð° ÑÐ²Ð»ÑÐµÑÑÑ Ð±Ð¸Ð¾Ð¼Ð°ÑÐºÐµÑÐ¾Ð¼ Ð¾ÑÐ²ÐµÑÐ° Ð½Ð° ÑÐµÑÐ°Ð¿Ð¸Ñ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾ÑÐ°Ð¼Ð¸ Ð¸Ð¼Ð¼ÑÐ½Ð½ÑÑ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½ÑÑ ÑÐ¾ÑÐµÐº Ð²Ð½Ðµ Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾ÑÑÐ¸ Ð¾Ñ ÑÐ¸Ð¿Ð° Ð¾Ð¿ÑÑÐ¾Ð»Ð¸. Ð¤ÐµÐ½Ð¾ÑÐ¸Ð¿ MSI ÑÐ°ÑÑÐ¾ Ð¾Ð±Ð½Ð°ÑÑÐ¶Ð¸Ð²Ð°ÐµÑÑÑ Ð² Ð¾Ð¿ÑÑÐ¾Ð»ÑÑ, Ð°ÑÑÐ¾ÑÐ¸Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ Ñ ÑÐ¸Ð½Ð´ÑÐ¾Ð¼Ð¾Ð¼ ÐÐ¸Ð½ÑÐ°, ÑÐ°ÐºÐ¸Ñ ÐºÐ°Ðº ÐºÐ¾Ð»Ð¾ÑÐµÐºÑÐ°Ð»ÑÐ½ÑÐ¹ ÑÐ°Ðº Ð¸ ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ð°Ð»ÑÐ½ÑÐ¹ ÑÐ°Ðº. Ð£Ð¶Ðµ ÑÑÑÐµÑÑÐ²ÑÐµÑ ÑÑÐ°Ð½Ð´Ð°ÑÑÐ¸Ð·Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¹ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½ÑÐ¹ Ð¼ÐµÑÐ¾Ð´ (ÐÐ¦Ð  Ñ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐ¸Ð¼ ÑÑÐ°Ð³Ð¼ÐµÐ½ÑÐ½ÑÐ¼ Ð°Ð½Ð°Ð»Ð¸Ð·Ð¾Ð¼ Ð¿ÑÐ¾Ð´ÑÐºÑÐ°) Ð´ÐµÑÐµÐºÑÐ¸Ð¸ Ð¼Ð¸ÐºÑÐ¾ÑÐ°ÑÐµÐ»Ð»Ð¸ÑÐ½Ð¾Ð¹ Ð½ÐµÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾ÑÑÐ¸ Ð² ÐºÐ¾Ð»Ð¾ÑÐµÐºÑÐ°Ð»ÑÐ½ÑÑ Ð¾Ð¿ÑÑÐ¾Ð»ÑÑ, Ð²ÐºÐ»ÑÑÐ°ÑÑÐ¸Ð¹ Ð² ÑÐµÐ±Ñ Ð½Ð°Ð±Ð¾Ñ Ð¸Ð· Ð¿ÑÑÐ¸ ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð¼Ð°ÑÐºÐµÑÐ¾Ð², ÐºÐ¾ÑÐ¾ÑÑÐµ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÑÑÑ ÑÐ¾Ð±Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾ÑÐ°ÑÐµÐ»Ð»Ð¸ÑÐ½ÑÐµ Ð»Ð¾ÐºÑÑÑ Ñ Ð¼Ð¾Ð½Ð¾Ð½ÑÐºÐ»ÐµÐ¾ÑÐ¸Ð´Ð½ÑÐ¼ Ð¿Ð¾Ð²ÑÐ¾ÑÐ¾Ð¼. ÐÐ´Ð½Ð°ÐºÐ¾ ÑÑÐ¸ Ð¼Ð°ÑÐºÐµÑÑ Ð¾Ð±Ð»Ð°Ð´Ð°ÑÑ Ð½Ð¸Ð·ÐºÐ¾Ð¹ ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐ½Ð¾ÑÑÑÑ Ð´Ð»Ñ ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ð¾Ð¸Ð´Ð½ÑÑ Ð¾Ð¿ÑÑÐ¾Ð»ÐµÐ¹, Ð¸ Ð¿Ð¾ÑÑÐ¾Ð¼Ñ Ð¿Ð¾Ð¸ÑÐº ÑÐ°ÐºÐ¸Ñ ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐ½ÑÑ Ð¼Ð°ÑÐºÐµÑÐ¾Ð² ÑÐ²Ð»ÑÐµÑÑÑ Ð°ÐºÑÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð·Ð°Ð´Ð°ÑÐµÐ¹.Â Ð¦ÐµÐ»Ñ ÑÐ°Ð±Ð¾ÑÑ â Ð¾Ð¿ÑÐ¸Ð¼Ð¸Ð·Ð°ÑÐ¸Ñ Ð¼ÐµÑÐ¾Ð´Ð¸ÐºÐ¸ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐ°ÑÐµÐ»Ð»Ð¸ÑÐ½Ð¾Ð¹ Ð½ÐµÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾ÑÑÐ¸ Ð² Ð¾Ð¿ÑÑÐ¾Ð»ÐµÐ²Ð¾Ð¹ ÐÐÐ ÑÐ°ÐºÐ° ÑÐµÐ»Ð° Ð¼Ð°ÑÐºÐ¸.Â ÐÐ°Ð·Ð½Ð°ÑÐµÐ½Ð¸Ðµ ÑÐµÑÑ-ÑÐ¸ÑÑÐµÐ¼Ñ â Ð´ÐµÑÐµÐºÑÐ¸Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐ°ÑÐµÐ»Ð»Ð¸ÑÐ½Ð¾Ð¹ Ð½ÐµÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾ÑÑÐ¸ Ð² Ð¾Ð±ÑÐ°Ð·ÑÐ°Ñ ÐÐÐ, Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½Ð¾Ð¹ Ð¸Ð· Ð¾Ð¿ÑÑÐ¾Ð»ÐµÐ¹ ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ñ. Ð ÑÐ°Ð±Ð¾ÑÐµ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÐµÐ½ Ð´Ð¸Ð·Ð°Ð¹Ð½ Ð¸ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð°Ð¿ÑÐ¾Ð±Ð°ÑÐ¸Ð¸ ÑÐµÑÑ-ÑÐ¸ÑÑÐµÐ¼Ñ, Ð²ÐºÐ»ÑÑÐ°ÑÑÐµÐ¹ 4 Ð¼Ð°ÑÐºÐµÑÐ°. Â ÐÑÐ»Ð¸ Ð¿ÑÐ¾Ð°Ð½Ð°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°Ð½Ñ 150 Ð¾Ð±ÑÐ°Ð·ÑÐ¾Ð², ÑÐ°ÑÑÐ¾ÑÐ° Ð²ÑÑÑÐµÑÐ°ÐµÐ¼Ð¾ÑÑÐ¸ MSI ÑÐ¾ÑÑÐ°Ð²Ð¸Ð»Ð° 12%.
description: The subject of the graduate qualification work: "Development of a test system for assessing microsatellite instability in uterine body cancer samples".Â Microsatellite instability (MSI) is a common change in the genotype of uterine corpus cancer tumor cells. It is a biomarker of response to therapy with immune checkpoint inhibitors regardless of tumor type. The MSI phenotype is often found in Lynch syndrome-associated tumors such as colorectal cancer and endometrial cancer. A standardized molecular method (PCR followed by fragment product analysis) for the detection of microsatellite instability in colorectal tumors already exists, including a set of five specific markers, which are microsatellite loci with a single nucleotide repeat. However, these markers have low specificity for endometrioid tumors, and therefore, the search for such specific markers is an urgent task.Â The aim of this work is to optimize the technique for determining microsatellite instability in tumor DNA of uterine corpus carcinoma.Â The purpose of the test system is to detect microsatellite instability in DNA samples isolated from endometrial tumors. The paper presents the design and results of validation of the test system including 4 markers.Â  150 samples were analyzed, the frequency of MSIs was 12%.