title: ÐÑÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ ÑÐµÐºÐ¾Ð¼Ð±Ð¸Ð½Ð°Ð½ÑÐ½Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° ÑÐµÐºÐ¾Ð¼Ð±Ð¸Ð½Ð°Ð·Ñ RAD51 ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð¸ Ñ Ð°ÑÐ°ÐºÑÐµÑÐ¸Ð·Ð°ÑÐ¸Ñ ÐµÑ ÐÐÐ-ÑÐ²ÑÐ·ÑÐ²Ð°ÑÑÐµÐ¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Isolation of recombinant RAD51 recombinase protein human and characterization of its DNA-binding activity
Creators: name: ÐÐ¾Ð½ÑÐ°ÑÐ¾Ð², ÐÐ²Ð°Ð½
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: ÐÐµÐ·Ð¾ÐºÑÐ¸ÑÐ¸Ð±Ð¾Ð½ÑÐºÐ»ÐµÐ¸Ð½Ð¾Ð²Ð°Ñ ÐºÐ¸ÑÐ»Ð¾ÑÐ° (ÐÐÐ)
ÑÐµÐ¿Ð°ÑÐ°ÑÐ¸Ñ ÐÐÐ
Ð¼ÐµÑÐ¾Ð´ Ð¾Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð·Ð°Ñ Ð²Ð°ÑÐ°
RAD51
Ñ ÑÐ¾Ð¼Ð°ÑÐ¾Ð³ÑÐ°ÑÐ¸Ñ
Deoxyribonucleic acid (DNA)
DNA repair
optical tweezers
chromatography

description: ÐÑÐ¾Ð±Ð»ÐµÐ¼Ð° Ð¾Ð½ÐºÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ð¹ - Ð¾Ð´Ð¸Ð½ Ð¸Ð· Ð³Ð»Ð°Ð²Ð½ÑÑ Ð²ÑÐ·Ð¾Ð²Ð¾Ð² Ð½Ð°ÑÐºÐ¸ XXI Ð²ÐµÐºÐ°. ÐÐ»Ñ Ð¿Ð¾Ð¸ÑÐºÐ° ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ Ð»ÐµÐºÐ°ÑÑÑÐ²Ð° ÑÐµÐ°Ð»Ð¸Ð·ÑÑÑÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¿Ð¾ Ð²ÑÐµÐ¼Ñ Ð¼Ð¸ÑÑ Ð² ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ Ð¾Ð±Ð»Ð°ÑÑÑÑ Ð¼ÐµÐ´Ð¸ÑÐ¸Ð½Ñ, ÑÐ¸Ð·Ð¸ÐºÐ¸, Ð±Ð¸Ð¾ÑÐ¸Ð¼Ð¸Ð¸. ÐÐ°Ð¸Ð±Ð¾Ð»ÐµÐµ ÑÑÑÐµÐºÑÐ¸Ð²Ð½ÑÐ¼ Ð¸Ð· Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½ÑÑ Ð¿ÑÑÐµÐ¹ Ð»ÐµÑÐµÐ½Ð¸Ñ Ð¼Ð¾Ð¶ÐµÑ ÑÑÐ°ÑÑ Ð²Ð¾ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¸Ðµ Ð¿ÑÐ°Ð²Ð¸Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÑÐµÐ¿Ð°ÑÐ°ÑÐ¸Ð¾Ð½Ð½ÑÑ Ð¿ÑÐ¾ÑÐµÑÑÐ¾Ð² Ð² ÐºÐ»ÐµÑÐºÐµ, ÐºÐ¾ÑÐ¾ÑÑÐµ Ð¼Ð¾Ð³ÑÑ Ð½Ð°ÑÑÑÐ°ÑÑÑÑ Ð² ÑÐ¸Ð»Ñ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ ÑÐ½Ð´Ð¾Ð³ÐµÐ½Ð½ÑÑ Ð¸ ÑÐºÐ·Ð¾Ð³ÐµÐ½Ð½ÑÑ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð². ÐÐ´Ð½Ð¸Ð¼ Ð¸Ð· Ð²Ð°Ð¶Ð½ÐµÐ¹ÑÐ¸Ñ ÑÐµÐ¿Ð°ÑÐ°ÑÐ¸Ð¾Ð½Ð½ÑÑ Ð±ÐµÐ»ÐºÐ¾Ð² ÑÐ²Ð»ÑÐµÑÑÑ RAD51, ÑÑÐ°ÑÑÐ²ÑÑÑÐ¸Ð¹ Ð² Ð²Ð¾ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¸Ð¸ Ð´Ð²ÑÑÑÐµÐ¿Ð¾ÑÐµÑÐ½ÑÑ ÑÐ°Ð·ÑÑÐ²Ð¾Ð² ÐÐÐ, Ð¸ ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½ÑÑ Ð²Ð¸Ð»Ð¾Ðº ÐÐÐ, Ð¿Ð¾Ð´Ð²ÐµÑÐ³Ð½ÑÐ²ÑÐ¸ÑÑÑ ÐºÐ¾Ð»Ð»Ð°Ð¿ÑÑ. ÐÐµ ÑÐ¼Ð¾ÑÑÑ Ð½Ð° ÐµÐ³Ð¾ ÐºÐ»ÑÑÐµÐ²ÑÑ ÑÑÐ½ÐºÑÐ¸Ñ, Ð±ÐµÐ»Ð¾Ðº Ð¾ÑÑÐ°ÐµÑÑÑ Ð¼Ð°Ð»Ð¾Ð¸Ð·ÑÑÐµÐ½Ð½ÑÐ¼, Ð² ÑÐ²ÑÐ·Ð¸ Ñ ÑÐµÐ¼ Ð² Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ Ð±ÑÐ»Ð¾ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ, Ð² ÑÐ¾Ð´Ðµ ÐºÐ¾ÑÐ¾ÑÐ¾Ð³Ð¾ Ð±ÐµÐ»Ð¾Ðº Ð²ÑÐ´ÐµÐ»ÑÐ»Ð¸ (Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»Ð¸ ÑÑÐ°Ð½ÑÑÐ¾ÑÐ¼Ð°ÑÐ¸Ñ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ ÑÐ»ÐµÐºÑÑÐ¾Ð¿Ð¾ÑÐ°ÑÐ¸Ð¸, ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ, Ð½Ð°ÑÐ°Ð±Ð¾ÑÐºÑ, Ð¾ÑÐ¸ÑÑÐºÑ Ð¼ÐµÑÐ¾Ð´Ð°Ð¼Ð¸ Ð°ÑÑÐ¸Ð½Ð½Ð¾Ð¹ Ð¸ Ð¸Ð¾Ð½Ð¾Ð¾Ð±Ð¼ÐµÐ½Ð½Ð¾Ð¹ ÑÑÐ¾Ð¼Ð°ÑÐ¾Ð³ÑÐ°ÑÐ¸Ð¸, ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ñ) Ð¸ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸Ð·Ð¾Ð²Ð°Ð»Ð¸ ÐµÐ³Ð¾ ÐÐÐ-ÑÐ²ÑÐ·ÑÐ²Ð°ÑÑÑÑ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ñ Ð¾Ð´Ð½Ð¾ÑÐµÐ¿Ð¾ÑÐµÑÐ½Ð¾Ð¹ Ð¸ Ð´Ð²ÑÑÐµÐ¿Ð¾ÑÐµÑÐ½Ð¾Ð¹ ÐÐÐ Ð¿ÑÐ¸ Ð¿Ð¾Ð¼Ð¾ÑÐ¸ Ð¼ÐµÑÐ¾Ð´Ð° Ð¾Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð·Ð°ÑÐ²Ð°ÑÐ° Ð½Ð° ÑÑÑÐ°Ð½Ð¾Ð²ÐºÐµ Â«ÐÐ°Ð·ÐµÑÐ½ÑÐ¹ Ð¿Ð¸Ð½ÑÐµÑÂ».
description: The problem of cancer is one of the main challenges for scientists of the XXI century. To find an effective cure, researches are realized all over the world in different fields of medicine, physics, biochemistry. The most effective of possible ways of treatment may be restoration of proper functioning of repair processes in the cell in case of their disorder arising due to various endogenous and exogenous factors. One of the most important repair proteins is RAD51, which is involved inÂ  repairing of double-stranded DNA breaks and replicative DNA forks that have undergone collapse. In spite of its key function, the protein remains poorly studied; therefore, in this work we conducted a study in which we isolated the protein (performed transformation by electroporation, expression, production, purification by affinity and anion-exchange chromatography, concentration) and characterized its DNA-binding activity with single-stranded and double-stranded DNA using the optical trapping method on the installation âOptical Tweezersâ.