cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Cytoskeletal requirements in Chlamydia trachomatis infection of host cells.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: Infection of genital epithelial cells by the closely related sexually transmitted pathogens Chlamydia trachomatis serovars E and L2 results in different clinical disease manifestations. Following entry into target host cells, individual vesicles containing chlamydiae fuse with one another to form one large inclusion. At the cellular level, the only obvious difference between these serovars is the time until inclusion maturation, which is 48 h for the invasive serovar L2 and 72 h for serovar E. To begin to define the intracellular events of these pathogens, the effect of cytoskeletal disruption on early endosome fusion and inclusion development in epithelial (HEC-1B) and fibroblast (McCoy) cells was analyzed by fluorescence microscopy. Disruption of microfilaments with cytochalasin D markedly reduced serovar E, but not serovar L2, infection of both cell lines. Conversely, microfilament as well as microtubule disruption, with colchicine or nocodazole, had no effect on serovar E inclusion development but resulted in the formation of multiple serovar L2 inclusions per cell during early and mid-development. Later in serovar L2 inclusion development (> 36 h postinfection), vesicles containing chlamydiae fused to form one large inclusion in the absence of an intact cytoskeleton. These results imply that (i) C. trachomatis serovar E may utilize a different pathway for uptake and development from serovar L2; (ii) these differences are consistent in both epithelial cells and fibroblasts; and (iii) the cytoskeleton plays a unique role in the infection of host cells by these two genital pathogens.
N2E7
Creators: name: Alyssah Clavien
Year: 2023
Subjects: subject: Mesoamerica
valueUri: https://core.tdar.org/browse/geographic-keyword/61/mesoamerica
subject: Basin of Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/3/basin-of-mexico
subject: Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/134/mexico
subject: Mexico (State / Territory)
valueUri: https://core.tdar.org/browse/geographic-keyword/326/mexico-state-territory
subject: United Mexican States (Country)
valueUri: https://core.tdar.org/browse/geographic-keyword/438/united-mexican-states-country
subject: Central Mexico, Teotihuacan Valley
valueUri: https://core.tdar.org/browse/geographic-keyword/7/central-mexico-teotihuacan-valley
subject: Central Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/135/central-mexico
subject: Teotihuacan Valley
valueUri: https://core.tdar.org/browse/geographic-keyword/154/teotihuacan-valley
subject: Teotihuacan
valueUri: https://core.tdar.org/browse/geographic-keyword/202/teotihuacan
subject: Valley of Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/44/valley-of-mexico
subject: Classic Period
valueUri: https://core.tdar.org/browse/temporal-keyword/125/classic-period
subject: Terminal Formative
valueUri: https://core.tdar.org/browse/temporal-keyword/67/terminal-formative
subject: Teotihuacan Period
valueUri: https://core.tdar.org/browse/temporal-keyword/7765/teotihuacan-period
subject: Early Classic
valueUri: https://core.tdar.org/browse/temporal-keyword/5560/early-classic
subject: Teotihuacan
valueUri: https://core.tdar.org/browse/culture-keyword/7527/teotihuacan
subject: Teotihuacano
valueUri: https://core.tdar.org/browse/culture-keyword/9224/teotihuacano
subject: Teotihuacan
valueUri: https://core.tdar.org/browse/site-name/92/teotihuacan
subject: Town / City
valueUri: https://core.tdar.org/browse/site-type/255/town-city
subject: Domestic Structure or Architectural Complex
valueUri: https://core.tdar.org/browse/site-type/251/domestic-structure-or-architectural-complex
subject: Settlements
valueUri: https://core.tdar.org/browse/site-type/252/settlements
subject: Systematic Survey
valueUri: https://core.tdar.org/browse/investigation-type/12/systematic-survey

description: Site Survey Form Scan for Square N2E7.
Identification and purification of a conserved heme-regulated hemoglobin-binding outer membrane protein from Haemophilus ducreyi.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: A hemoglobin-binding protein (HgbA) from Haemophilus ducreyi was identified and purified. The 100-kDa HgbA was detected in all strains of H. ducreyi tested, and a somewhat larger hemoglobin-binding protein was found in one strain of Haemophilus influenzae. HgbA was purified and the amino acid sequence of the N terminus of HgbA revealed no significant homologies with known proteins. Two different antisera to HgbA from H. ducreyi 35000 recognized HgbA proteins from all tested H. ducreyi strains; they did not recognize proteins from the H. influenzae strain. Expression of HgbA was regulated by the level of heme but not by iron present in the medium. Animal species of hemoglobin competed with iodinated human hemoglobin for binding to whole cells of H. ducreyi and supported the growth of H. ducreyi. The lack of immunological cross-reactivity and the differences in hemoglobin specificities between the H. ducreyi and the H. influenzae hemoglobin-binding proteins suggest that they are unrelated.
Isolation and identification of a glutathione peroxidase homolog gene, gpxA, present in Neisseria meningitidis but absent in Neisseria gonorrhoeae.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: Antioxidant enzymes are thought to be important for the survival of pathogenic Neisseria species. We isolated a glutathione peroxidase-related gene (gpxA) from Neisseria meningitidis FAM20. The N. meningitidis glutathione peroxidase homolog was 49 to 57% identical to seven other glutathione peroxidase family members over a 49-amino-acid region which is conserved among various species. The gpxA sequence was present in all 7 meningococcal strains tested but absent in 10 gonococcal strains and 6 nonpathogenic neisserial strains as determined by Southern hybridization. The homology of gpxA to mammalian glutathione peroxidases and the presence of this gene specifically in the meningococcus suggest that it is important in the cellular metabolism or defense processes particular to this pathogen.
High energy photon polarimeter for astrophysics
Creators: name: Eingorn, Maxim
name: Fernando, Lakma
name: Vlahovic, Branislav
name: Ilie, Cosmin
name: Wojtsekhowski, Bogdan
name: Urciuoli, Guido Maria
name: De Persio, Fulvio
name: Meddi, Franco
name: Nelyubin, Vladimir
Publisher: name: arXiv
Year: 2015
Subjects: subject: High Energy Astrophysical Phenomena (astro-ph.HE)
subject: Instrumentation and Methods for Astrophysics (astro-ph.IM)
subject: Instrumentation and Detectors (physics.ins-det)
subject: FOS: Physical sciences

description: A high-energy photon polarimeter for astrophysics studies in the energy range from 10 MeV to 800 MeV is considered. The proposed concept uses a stack of silicon micro-strip detectors where they play the roles of both a converter and a tracker. The purpose of this paper is to outline the parameters of such a polarimeter and to estimate the productivity of measurements. Our study supported by a Monte Carlo simulation shows that with a one-year observation period the polarimeter will provide 6% accuracy of the polarization degree for photon energies above 100 MeV, which would be a significant advance relative to the currently explored energy range of a few MeV. The proposed polarimeter design could easily be adjusted to the specific photon energy range to maximize efficiency if needed.
description: 19 pages, 7 figures
S4W1
Creators: name: Alyssah Clavien
Year: 2023
Subjects: subject: Mesoamerica
valueUri: https://core.tdar.org/browse/geographic-keyword/61/mesoamerica
subject: Basin of Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/3/basin-of-mexico
subject: Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/134/mexico
subject: Mexico (State / Territory)
valueUri: https://core.tdar.org/browse/geographic-keyword/326/mexico-state-territory
subject: United Mexican States (Country)
valueUri: https://core.tdar.org/browse/geographic-keyword/438/united-mexican-states-country
subject: Central Mexico, Teotihuacan Valley
valueUri: https://core.tdar.org/browse/geographic-keyword/7/central-mexico-teotihuacan-valley
subject: Central Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/135/central-mexico
subject: Teotihuacan Valley
valueUri: https://core.tdar.org/browse/geographic-keyword/154/teotihuacan-valley
subject: Teotihuacan
valueUri: https://core.tdar.org/browse/geographic-keyword/202/teotihuacan
subject: Valley of Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/44/valley-of-mexico
subject: Classic Period
valueUri: https://core.tdar.org/browse/temporal-keyword/125/classic-period
subject: Terminal Formative
valueUri: https://core.tdar.org/browse/temporal-keyword/67/terminal-formative
subject: Teotihuacan Period
valueUri: https://core.tdar.org/browse/temporal-keyword/7765/teotihuacan-period
subject: Early Classic
valueUri: https://core.tdar.org/browse/temporal-keyword/5560/early-classic
subject: Teotihuacan
valueUri: https://core.tdar.org/browse/culture-keyword/7527/teotihuacan
subject: Teotihuacano
valueUri: https://core.tdar.org/browse/culture-keyword/9224/teotihuacano
subject: Teotihuacan
valueUri: https://core.tdar.org/browse/site-name/92/teotihuacan
subject: Town / City
valueUri: https://core.tdar.org/browse/site-type/255/town-city
subject: Domestic Structure or Architectural Complex
valueUri: https://core.tdar.org/browse/site-type/251/domestic-structure-or-architectural-complex
subject: Settlements
valueUri: https://core.tdar.org/browse/site-type/252/settlements
subject: Systematic Survey
valueUri: https://core.tdar.org/browse/investigation-type/12/systematic-survey

description: Site Survey Form Scan for Square S4W1.
Manufacture of organic compost using technology
Creators: name: Moustafa, Mariam
Publisher: name: figshare
Year: 2024
Subjects: classificationCode: 300208
subject: Farm management, rural management and agribusiness
classificationCode: 409901
subject: Agricultural engineering
classificationCode: 300210
subject: Sustainable agricultural development
classificationCode: 400405
subject: Food engineering
classificationCode: 400999
subject: Electronics, sensors and digital hardware not elsewhere classified

description: The world faces the dual challenges of developing productive crops and managing waste, which contributes to greenhouse gas emissions and climate change. This project addresses these issues by accelerating organic waste decomposition through an in-vessel composting process, producing high-quality organic compost that enriches soil and agricultural land. The project utilizes an integrated Arduino system, comprising sensors, devices, and code, to control temperature, moisture, and ventilation. By doing so, it reduces decomposition duration by over 50% compared to traditional methods. This innovative approach offers a cheaper, healthier, and more environmentally friendly alternative to traditional fertilizers, benefiting farmers, the Egyptian economy, and the environment.
A solution of the Erdos-Ulam problem on rational distance sets assuming the Bombieri-Lang conjecture
Creators: name: Shaffaf, Jafar
Publisher: name: arXiv
Year: 2015
Subjects: subject: Number Theory (math.NT)
subject: Algebraic Geometry (math.AG)
subject: FOS: Mathematics
subject: 11G99, 14G05, 14J29, 30D35, 52C10

description: A rational distance set in the plane is a point set which has the property that all pairwise distances between its points are rational. Erd\H os and Ulam conjectured in 1945 that there is no dense rational distance set in the plane. In this paper we associate an algebraic surface in $\mathbb{P}^3$, that we call a distance surface, to any finite rational distance set in the plane. Under a mild condition, we prove that a distance surface is always a surface of general type. From this, we deduce that the Bombieri-Lang conjecture in arithmetic algebraic geometry (restricted to the classes of surfaces) implies an answer to the Erd\H os-Ulam problem. Combined with the results of Solymosi and de Zeeuw, our proofs lead to the following stronger statement: for $S$ a rational distance set with infinitely many points, we have Either, all but at most four points of $S$ are on a line, Or, all but at most three points of $S$ are on a circle.
description: 13 pages, analysis of singularity at infinity was added. Also a conjecture was added at the end of the paper
N7E1
Creators: name: Alyssah Clavien
Year: 2023
Subjects: subject: Mesoamerica
valueUri: https://core.tdar.org/browse/geographic-keyword/61/mesoamerica
subject: Basin of Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/3/basin-of-mexico
subject: Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/134/mexico
subject: Mexico (State / Territory)
valueUri: https://core.tdar.org/browse/geographic-keyword/326/mexico-state-territory
subject: United Mexican States (Country)
valueUri: https://core.tdar.org/browse/geographic-keyword/438/united-mexican-states-country
subject: Central Mexico, Teotihuacan Valley
valueUri: https://core.tdar.org/browse/geographic-keyword/7/central-mexico-teotihuacan-valley
subject: Central Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/135/central-mexico
subject: Teotihuacan Valley
valueUri: https://core.tdar.org/browse/geographic-keyword/154/teotihuacan-valley
subject: Teotihuacan
valueUri: https://core.tdar.org/browse/geographic-keyword/202/teotihuacan
subject: Valley of Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/44/valley-of-mexico
subject: Classic Period
valueUri: https://core.tdar.org/browse/temporal-keyword/125/classic-period
subject: Terminal Formative
valueUri: https://core.tdar.org/browse/temporal-keyword/67/terminal-formative
subject: Teotihuacan Period
valueUri: https://core.tdar.org/browse/temporal-keyword/7765/teotihuacan-period
subject: Early Classic
valueUri: https://core.tdar.org/browse/temporal-keyword/5560/early-classic
subject: Teotihuacan
valueUri: https://core.tdar.org/browse/culture-keyword/7527/teotihuacan
subject: Teotihuacano
valueUri: https://core.tdar.org/browse/culture-keyword/9224/teotihuacano
subject: Teotihuacan
valueUri: https://core.tdar.org/browse/site-name/92/teotihuacan
subject: Town / City
valueUri: https://core.tdar.org/browse/site-type/255/town-city
subject: Domestic Structure or Architectural Complex
valueUri: https://core.tdar.org/browse/site-type/251/domestic-structure-or-architectural-complex
subject: Settlements
valueUri: https://core.tdar.org/browse/site-type/252/settlements
subject: Systematic Survey
valueUri: https://core.tdar.org/browse/investigation-type/12/systematic-survey

description: Site Survey Form Scan for Square N7E1.
Pepadb: Putting the FAIR Principles into Practice
Creators: name: Romero Garcia, Galo
name: Garrido-Cordero, José Angel
name: Odriozola, Carlos P.
Publisher: name: Zenodo
Year: 2022
Subjects: Archaeology
FAIR
Data reuse

description: The main interest in the publication of spatial indexed archaeological data is to offer users the possibility of querying, downloading and/or accessing them in external applications. This apps are aimed to model, analyze, display or generate new geospatial data, information and value-added resources, both in the social and economic spheres. Given the extremely high cost of generating geographic information, the reuse of this data by users is highly expected. As the result of not complying with the FAIR EU policy, the already existing spatial indexed archaeological data are not easily accessible and therefore reusable, neither to the public, nor to the academia (Sobotkova, 2018). PEPAdb (Prehistoric Europe’s Personal Adornment) is a multidisciplinary and open research project which has materialized in an SDI (Spatial Data Infrastructure) and a web-app for the online publication of georeferenced archaeological scientific data referring to personal adornment in European Recent Prehistory. It accounts for thousands of records on Prehistoric Personal Adornment from over 900 archaeological sites on the Iberian Peninsula, giving access not only to thematic cartographies, but also to raw data and empirical data sets through an online cartographic viewer, and a database query app. This initiative aims to comply with the FAIR principles, placing special emphasis on data reusability. Data repositories (such as Dataverse, among others) can generate a formal citation for each piece of data according to specific standards, in addition to automatically assigning the DOI (Document Object Identifier) and the so-called persistent identifiers, providing a greater accessibility to (meta)data (Wilkinson, 2016). Referenced cites: Sobotkova, A. (2018). Sociotechnical Obstacles to Archaeological Data Reuse. Advances in Archaeological Practice, 6(02):1-8. DOI: 10.1017/aap.2017.37. Wilkinson, M.D. et al. (2016). The FAIR Guiding Principles for scientific data management and stewardship. Scientific Data, 3, 160018. DOI: https://doi.org/10.1038/sdata.2016.18
Reactivation of arthritis induced by small bowel bacterial overgrowth in rats: role of cytokines, bacteria, and bacterial polymers.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: Arthritis is often associated with intestinal diseases, but the etiology is not known. We developed a rat model whereby arthritis was reactivated by experimental small bowel bacterial overgrowth (SBBO). Self-limited monoarticular arthritis was induced by intra-articular injection of 2 micrograms of rhamnose peptidoglycan-polysaccharide derived from group A streptococci into the ankle joints in female Lewis rats. Eleven days after intra-articular injection, when swelling was resolving, experimental SBBO induced by surgical creation of jejunal self-filling blind loops reactivated arthritis, but SBBO induced by creation of self-emptying blind loops, which minimally increases luminal bacteria, and sham operation did not (P < 0.001). Increased joint diameters in rats with self-filling blind loops persisted for at least 56 days after surgery. Reactivation of arthritis due to SBBO was prevented by anti-tumor necrosis factor alpha antiserum and interleukin 1 receptor antagonist (P < 0.001), indicating that these cytokines mediate joint swelling secondary to intestinal injury. Recombinant bactericidal/permeability-increasing protein, an agent which neutralizes endotoxin, and metronidazole, which is active against anaerobic bacteria, prevented arthritis (P < 0.001), but polymyxin B (which also neutralizes endotoxin) and gentamicin had no effect. Mutanolysin, an enzyme which degrades peptidoglycan-polysaccharide from group A streptococci, exacerbated arthritis for the first 6 days but then diminished joint swelling from 12 to 21 days after surgery (P < 0.001). These studies introduce a reproducible animal model of reactivation of arthritis secondary to intestinal injury and demonstrate a role for bacterial products from endogenous enteric organisms.
ESMARConf 2023: Advanced GitHub Workshop
Creators: name: Pritchard, Chris
name: Felton, Liz
Publisher: name: Zenodo
Year: 2023

description: In this advanced GitHub workshop, we’ll bring you on a journey from refreshing your knowledge of basic terms through to understanding some of the nuts and bolts of Git. You’ll learn how to use the command line to manipulate git repositories, and even get working on some continuous integration and delivery, so that your changes can be available to the world in real-time. We would need you to have a working knowledge of git and either GitHub, or another similar platform, alternatively, this workshop follows on from the “Introduction to Git and GitHub” workshop so feel free to sign up for both!

 Learning objectives:
 - To define terminology relating to Git
 - To discuss the structure of a Git repository
 - To identify basic commands on windows, Linux, and mac OS
 - To describe the use of Continuous Integration and Continuous Delivery in modern tool development
 - To use the command line interface to Git to undertake basic repository related tasks, for example, create a new branch
 - To use git hooks and github actions to automatically run actions at various stages in a git workflow
 - To sign commits using GPG

 Target audience:
 A working knowledge of git and github (or similar alternative such as gitlab, gitea, or forjeo) OR completion of the basic github workshop.

 Prerequisites:
 Git, a GitHub Account, GNUPG, GPG Tools (Mac) or GPG4WIN (Windows) (https://gnupg.org/ / https://gpgtools.org/ / https://www.gpg4win.org/) for mac users with homebrew GNPUG can be installed with “brew install gnupg” or by following instructions here: https://mikeross.xyz/gpg-without-gpgtools-on-mac/).
Swine model of Haemophilus ducreyi infection.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: Haemophilus ducreyi is a strict human pathogen that causes sexually transmitted genital ulcer disease. We infected domestic swine with H. ducreyi 35000, resulting in the development of cutaneous ulcers histologically resembling human chancroid lesions. Intraepidermal lesions progressed from pustules to ulcers containing polymorphonuclear leukocytes and were accompanied by a dermal inflammatory infiltrate containing T cells and macrophages. H. ducreyi was recovered from lesions up to 17 days after inoculation, and pigs did not develop immunity to reinfection with the challenge strain. Features of the model include inoculation through abrasions in the epidermis, ambient housing temperatures for infected pigs, the ability to deliver multiple different inocula to a single host, and the availability of monoclonal antibodies against porcine immune cells permitting immunohistochemical characterization of the host immune response to H. ducreyi infection.
N7W4
Creators: name: Alyssah Clavien
Year: 2023
Subjects: subject: Mesoamerica
valueUri: https://core.tdar.org/browse/geographic-keyword/61/mesoamerica
subject: Basin of Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/3/basin-of-mexico
subject: Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/134/mexico
subject: Mexico (State / Territory)
valueUri: https://core.tdar.org/browse/geographic-keyword/326/mexico-state-territory
subject: United Mexican States (Country)
valueUri: https://core.tdar.org/browse/geographic-keyword/438/united-mexican-states-country
subject: Central Mexico, Teotihuacan Valley
valueUri: https://core.tdar.org/browse/geographic-keyword/7/central-mexico-teotihuacan-valley
subject: Central Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/135/central-mexico
subject: Teotihuacan Valley
valueUri: https://core.tdar.org/browse/geographic-keyword/154/teotihuacan-valley
subject: Teotihuacan
valueUri: https://core.tdar.org/browse/geographic-keyword/202/teotihuacan
subject: Valley of Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/44/valley-of-mexico
subject: Classic Period
valueUri: https://core.tdar.org/browse/temporal-keyword/125/classic-period
subject: Terminal Formative
valueUri: https://core.tdar.org/browse/temporal-keyword/67/terminal-formative
subject: Teotihuacan Period
valueUri: https://core.tdar.org/browse/temporal-keyword/7765/teotihuacan-period
subject: Early Classic
valueUri: https://core.tdar.org/browse/temporal-keyword/5560/early-classic
subject: Teotihuacan
valueUri: https://core.tdar.org/browse/culture-keyword/7527/teotihuacan
subject: Teotihuacano
valueUri: https://core.tdar.org/browse/culture-keyword/9224/teotihuacano
subject: Teotihuacan
valueUri: https://core.tdar.org/browse/site-name/92/teotihuacan
subject: Town / City
valueUri: https://core.tdar.org/browse/site-type/255/town-city
subject: Domestic Structure or Architectural Complex
valueUri: https://core.tdar.org/browse/site-type/251/domestic-structure-or-architectural-complex
subject: Settlements
valueUri: https://core.tdar.org/browse/site-type/252/settlements
subject: Systematic Survey
valueUri: https://core.tdar.org/browse/investigation-type/12/systematic-survey

description: Site Survey Form Scan for Square N7W4.
Cloning of a DNA fragment encoding a heme-repressible hemoglobin-binding outer membrane protein from Haemophilus influenzae.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: Haemophilus influenzae is able to use hemoglobin as a sole source of heme, and heme-repressible hemoglobin binding to the cell surface has been demonstrated. Using an affinity purification methodology, a hemoglobin-binding protein of approximately 120 kDa was isolated from H. influenzae type b strain HI689 grown in heme-restricted but not in heme-replete conditions. The isolated protein was subjected to N-terminal amino acid sequencing, and the derived amino acid sequence was used to design corresponding oligonucleotides. The oligonucleotides were used to probe a Southern blot of EcoRI-digested HI689 genomic DNA. A hybridizing band of approximately 4.2 kb was successfully cloned into pUC19. Using a 1.9-kb internal BglII fragment of the 4.2-kb clone as a probe, hybridization was seen in both typeable and nontypeable H. influenzae but not in other bacterial species tested. Following partial nucleotide sequencing of the 4.2-kb insert, a putative open reading frame was subcloned into an expression vector. The host Escherichia coli strain in which the cloned fragment was expressed bound biotinylated human hemoglobin, whereas binding of hemoglobin was not detected in E. coli with the vector alone. In conclusion, we hypothesize that the DNA fragment encoding an approximately 120-kDa heme-repressible hemoglobin-binding protein mediates one step in the acquisition of hemoglobin by H. influenzae in vivo.
Uniform Bounds for Weil-Petersson Curvatures
Creators: name: Wolf, Michael
name: Wu, Yunhui
Publisher: name: arXiv
Year: 2015
Subjects: subject: Differential Geometry (math.DG)
subject: Complex Variables (math.CV)
subject: Geometric Topology (math.GT)
subject: FOS: Mathematics

description: We find bounds for Weil-Petersson holomorphic sectional curvature, and the Weil-Petersson curvature operator in several regimes, that do not depend on the topology of the underlying surface. Among other results, we show that the minimal (most negative) eigenvalue of the curvature operator at any point in the Teichmüller space $\Teich(S_g)$ of a closed surface $S_g$ of genus $g$ is uniformly bounded away from zero. Restricting to a thick part of $\Teich(S_g)$, we show that the minimal eigenvalue is uniformly bounded below by an explicit constant which does not depend on the topology of the surface but only on the given bound on injectivity radius. We also show that the minimal Weil-Petersson holomorphic sectional curvature of a sufficiently thick hyperbolic surface is comparable to $-1$.
description: To appear in Proceedings of the London Mathematical Society, 41 pages
Expression of the CopB outer membrane protein by Moraxella catarrhalis is regulated by iron and affects iron acquisition from transferrin and lactoferrin.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: The amino acid sequence of the cell-surface-exposed, 81-kDa CopB outer membrane protein of Moraxella catarrhalis was found to be similar to those of TonB-dependent outer membrane proteins of other gram-negative bacteria. Expression of CopB was affected by the availability of iron in the growth medium, and the extent of overexpression of CopB in response to iron limitation varied widely among the M. catarrhalis strains tested. Wild-type M. catarrhalis strains were found to be able to utilize ferric citrate, transferrin, lactoferrin, and heme as sources of iron for growth in vitro. However, an isogenic copB mutant was severely impaired in its ability to utilize transferrin and lactoferrin as sole sources of iron for growth, whereas this same mutant grew similarly to the wild-type parent strain when supplied with ferric citrate as the iron source. The copB mutant was not significantly different from its wild-type parent strain in its ability to bind transferrin and lactoferrin. In addition, the wild-type parent strain and the copB mutant exhibited equivalent rates of uptake of 55Fe from ferric citrate. However, the copB mutant was markedly less able than the wild-type strain to take up 55Fe from transferrin and lactoferrin. These results indicate that lack of expression of the CopB protein exerts a direct or indirect effect on the ability of M. catarrhalis to utilize iron bound to certain carrier proteins.
Hemin-induced modifications of the antigenicity and hemin-binding capacity of Porphyromonas gingivalis lipopolysaccharide.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: Previous studies have shown that the physical, biochemical, and antigenic properties of the bacterial outer membrane are profoundly influenced by the growth environment. In the present study, the effects of growth in hemin-replete (H+) and hemin-depleted (H-) media on the lipopolysaccharide (LPS) of the oral pathogen Porphyromonas gingivalis were investigated. Our studies show that LPS from P. gingivalis cultured in H+ media (H+LPS) expressed additional low-molecular-mass antigens, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot (immunoblot) analysis. Particularly evident was a 26-kDa antigen (26 LPSC) that was lost from the LPS upon transfer of P. gingivalis into H- media. The loss of the 26 LPSC was accompanied by a marked reduction in the hemin-binding capacity of the LPS. The 26 LPSC was refractory to Coomassie blue staining and proteinase K digestion. H+LPS from strain W50/BE1, a nonpigmented pleiotropic strain, lacked the 26 LPSC and did not bind hemin. Polyclonal antiserum raised to whole-cell antigens of P. gingivalis A7436, W83, and HG405 grown in H+ media, but not in H- media, recognized the 26 LPSC in the purified H+LPS from any of the three strains. The immunoreactivities of sera from humans with (n = 24) or without (n = 25) periodontitis to the 26 LPSC and other H+LPS determinants were analyzed by Western blot. Overall, 75% of adult periodontitis patient sera reacted with multiple bands in the H+LPS stepladder, particularly in the range of 14 to 27 kDa. In contrast, only 20% of control sera reacted faintly with H+LPS bands in the range 27 to 34 kDa. The 26 LPSC was recognized by over 40% of sera from adult patients with periodontitis and none of the healthy control sera. Taken together, these results suggest that the antigenicity and hemin-binding properties of P. gingivalis LPS can be modified by growth in H+ media.
Identification and purification of a hemoglobin-binding outer membrane protein from Neisseria gonorrhoeae.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: The majority of in vitro-grown Neisseria gonorrhoeae strains were unable to use hemoglobin as the sole source of iron for growth (Hgb-), but a minor population was able to do so (Hgb+). The ability of Hgb+ gonococci to utilize hemoglobin as the iron source was associated with the expression of an iron-repressible 89-kDa hemoglobin-binding protein in the outer membrane. The N-terminal amino acid sequence of this protein revealed amino acids, from positions 2 to 16, identical to those of HpuB, an 85 kDa iron-regulated hemoglobin-haptoglobin utilization outer membrane protein of Neisseria meningitidis. Isogenic mutants constructed by allelic replacement with a meningococcal hpu::mini-Tn3erm construct no longer expressed the 89-kDa protein. Mutants could not utilize hemoglobin to support growth but still grew on heme. Thus, the gonococcal HpuB homolog is a functional hemoglobin receptor and is essential for growth with hemoglobin.
N4W3
Creators: name: Alyssah Clavien
Year: 2023
Subjects: subject: Mesoamerica
valueUri: https://core.tdar.org/browse/geographic-keyword/61/mesoamerica
subject: Basin of Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/3/basin-of-mexico
subject: Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/134/mexico
subject: Mexico (State / Territory)
valueUri: https://core.tdar.org/browse/geographic-keyword/326/mexico-state-territory
subject: United Mexican States (Country)
valueUri: https://core.tdar.org/browse/geographic-keyword/438/united-mexican-states-country
subject: Central Mexico, Teotihuacan Valley
valueUri: https://core.tdar.org/browse/geographic-keyword/7/central-mexico-teotihuacan-valley
subject: Central Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/135/central-mexico
subject: Teotihuacan Valley
valueUri: https://core.tdar.org/browse/geographic-keyword/154/teotihuacan-valley
subject: Teotihuacan
valueUri: https://core.tdar.org/browse/geographic-keyword/202/teotihuacan
subject: Valley of Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/44/valley-of-mexico
subject: Classic Period
valueUri: https://core.tdar.org/browse/temporal-keyword/125/classic-period
subject: Terminal Formative
valueUri: https://core.tdar.org/browse/temporal-keyword/67/terminal-formative
subject: Teotihuacan Period
valueUri: https://core.tdar.org/browse/temporal-keyword/7765/teotihuacan-period
subject: Early Classic
valueUri: https://core.tdar.org/browse/temporal-keyword/5560/early-classic
subject: Teotihuacan
valueUri: https://core.tdar.org/browse/culture-keyword/7527/teotihuacan
subject: Teotihuacano
valueUri: https://core.tdar.org/browse/culture-keyword/9224/teotihuacano
subject: Teotihuacan
valueUri: https://core.tdar.org/browse/site-name/92/teotihuacan
subject: Town / City
valueUri: https://core.tdar.org/browse/site-type/255/town-city
subject: Domestic Structure or Architectural Complex
valueUri: https://core.tdar.org/browse/site-type/251/domestic-structure-or-architectural-complex
subject: Settlements
valueUri: https://core.tdar.org/browse/site-type/252/settlements
subject: Systematic Survey
valueUri: https://core.tdar.org/browse/investigation-type/12/systematic-survey

description: Site Survey Form Scan for Square N4W3.
Design of W-band coplanar waveguide power amplifier in 0.1μm Gallium arsenide process
Creators: name: Chau, Quoc Toan
Publisher: name: Macquarie University
Year: 2022
Subjects: Other education not elsewhere classified

description: The widespread use of modern technologies is fueling demand for faster, more efficient and greater capacity wireless networks for which the millimeter-wave frequency range such as W-band (75-110 GHz) is explored. There are several design challenges in W-band like substantial path loss, atmospheric interference, and poor obstacle penetration. Therefore, at W-band a power amplifier (PA) will play an important role in determining the distance of signal travel. Some microstrip W-band PAs are reported in the literature. However, only a few coplanar waveguide (CPW) based PAs are available in the literature, although in general CPW technique has certain advantages over microstrip lines at high frequencies such as W-band. In this thesis, a W-band CPW amplifier design is targeted. It is noted that the ground planes occupy a large area. To make the size compact, different layout options are explored. A simple CPW line is studied in HFSS. Then different layouts of a simple CPW FET and a Cascode unit cell are investigated in AWR to reduce the circuit size. Finally, a compact CPW amplifier design is presented and its suitability for the W-band application is determined. The designs are implemented in WIN Semiconductor’s 0.1 μm Gallium Arsenide (GaAs) process codenamed PP10-20.
Neoliberal agency, relational agency, and the representation of the agentic child in the Sociology of Childhood
Creators: name: Clark, Jessica
Publisher: name: University of Westminster
Year: 2021

description: This critical commentary fulfils the regulations laid out by the University of Westminster as part of the submission for the award of PhD by Published Works. It accompanies nine published works that form the body of this submission and outlines their coherence, originality and contribution to knowledge. This body of work were published over a period of 6 years (2013-2019) and collectively is situated within, and at the intersection of the fields of sociology and childhood studies. In these works I interrogate the canonical concept of agency and I argue that the inherent contradictions of how agency is conceptualised, has more to do with the neoliberal model of agency being applied than whether children can and do exercise agency. The spaces of popular culture and of research with children are both contexts within which, dominant images of the child are reified and indeed produced. They offer both serious and playful spaces to critique and to reimagine the concept of agency and the potential that it offers. By considering explicitly how agency intersects with related concepts of vulnerability, care, participation, relationships and voice this body of work demonstrates there is significant analytical value in the concept of agency as applied to children and childhood. However, neoliberal models, which prize self-interested, individualistic, independent autonomy fail to acknowledge the lived realities of children’s lives or their situated and embedded nature in families, peer networks and assemblages of people and things. Like adults, children are not wholly agentic, nor are they utterly powerless. Rather, as I argue in this commentary, the agency of children is situated, contextual, contingent, and most importantly, relational; emerging in interesting and unexpected ways.
Classification of Symmetry-Protected Phases for Interacting Fermions in Two Dimensions
Creators: name: Cheng, Meng
name: Bi, Zhen
name: You, Yi-Zhuang
name: Gu, Zheng-Cheng
Publisher: name: arXiv
Year: 2015
Subjects: subject: Strongly Correlated Electrons (cond-mat.str-el)
subject: FOS: Physical sciences

description: Recently, it has been shown that two-dimensional bosonic symmetry-protected topological(SPT) phases with on-site unitary symmetry $G$ can be completely classified by the group cohomology class $H^3(G, \mathrm{U}(1))$. Later, group super-cohomology class was proposed as a partial classification for SPT phases of interacting fermions. In this work, we revisit this problem based on the mathematical framework of $G$-extension of unitary braided tensor category(UBTC) theory. We first reproduce the partial classifications given by group super-cohomology, then we show that with an additional $H^1(G, \mathbb{Z}_2)$ structure, a complete classification of SPT phases for two-dimensional interacting fermion systems for a total symmetry group $G\times\mathbb{Z}_2^f$ can be achieved. We also discuss the classification of interacting fermionic SPT phases protected by time-reversal symmetry.
description: references added; published version
Über die Auswirkungen einer Ozonbehandlung von Boden auf die natürliche organische Bodenmatrix
Creators: name: Kautt, Cornelia C.
Publisher: name: Universität Karlsruhe (TH)
Year: 1997

description: Die Auswirkungen einer Ozonbehandlung von Boden auf die natürliche organische Bodensubstanz und auf die Zusammensetzung des mit Wasser eluierbaren Anteils wurden mittels summarischer, chemischer und biologischer Meßgrößen und einer auf GC/MS, HPLC und IC beruhenden Einzelstoffanalytik untersucht. Im wäßrigen Eluat des ozonbehandelten Bodens konnten mehr als 130 organische Einzelverbindungen identifiziert werden, die zwischen 10-70 % des löslichen organischen Kohlenstoffanteils darstellten. Als Hauptprodukte wurden Carbonylverbindungen, kurzkettige Dicarbonsäuren, Benzolpolycarbonsäuren und als Endprodukte Oxal- und Ameisensäure nachgewiesen. Ca. 1 % des Ausgangskohlenstoffgehalts wurde während der Ozonbehandlung in Form flüchtiger Carbonylverbindungen über das Abgas ausgetragen. Bei niedrigen Ozondosen wurde der organische Kohlenstoffanteil des Bodens in großem Ausmaß mobilisiert, womit ein starkes Absinken des pH-Werts auf einen Wert von bis zu 3 verbunden war. Längere Ozonbehandlung führte zu einer deutlichen Abnahme des löslichen Kohlenstoffgehalts infolge der zunehmenden Mineralisierung von bis zu 90 % des Ausgangskohlenstoffgehalts. Parallel hierzu stieg auch der pH-Wert wieder an. Die Mineralisierung des organischen Stickstoffanteils führte überwiegend zur Bildung von Ammoniak, verlief aber deutlich langsamer als die des organischen Kohlenstoffanteils, so daß sich stickstoffhaltige organische Verbindungen im wäßrigen Eluat anreicherten. Die Versauerung des Bodens und die oxidative Zerstörung der komplexierenden organischen Makromoleküle führte zu einer beträchtlichen Freisetzung von Schwermetallen in das wäßrige Eluat; teilweise wurde das gesamte Schwermetallinventar des Bodens in die wäßrige Phase mobilisiert. Die Ozonbehandlung bis zu einem Verbrauch von 40 g pro kg Boden zeigte keine wesentliche Auswirkung auf die Keimzahl. Die Gesamtkeimzahl des ozonbehandelten Bodens nahm maximal um zwei Zehnerpotenzen ab. Sowohl der ozonbehandelte Boden als auch das wäßrige Eluat wiesen eine bessere biologische Abbaubarkeit auf, wobei bei niedrigen Ozondosen vermutlich zwischenzeitlich toxische Produkte entstanden, wie der Leuchtbakterienhemmtest zeigte.
Identification of murine protective epitopes on the Porphyromonas gingivalis fimbrillin molecule.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: Fimbriae from Porphyromonas gingivalis are believed to play an important role in the pathogenesis of periodontal diseases. The aim of the present study was to identify the fimbrial protective T-cell epitopes in CBA/J mice. A truncated protein corresponding to amino acids 1 to 198, PgF1-198, was generated and allowed us to demonstrate that the N terminus of the protein contains T-cell epitopes. With synthetic peptides, an immunodominant sequence was identified between amino acids 103 and 122. The corresponding peptide, PgF-P8, induced T-cell proliferation after in vitro restimulation of in vivo-primed cells, giving a stimulation index comparable to the one obtained with r-fimbrillin, and induced production of both Th1 and Th2 cytokines. Growth supernatant contained significant levels of interleukin 2 (IL-2), gamma interferon, IL-4 (28 pg/ml), and tumor necrosis factor alpha. Immunization of mice with r-fimbrillin, PgF1-198, and PgF-P8 induced production of antibodies specific to r-fimbrillin and PgF-P8. In addition, by using the mouse chamber model we found that mice immunized with PgF-P8 were dramatically protected against a normally lethal injection of P. gingivalis. Animals immunized with PgF-P8 40 days prior to challenge showed a 60% survival rate when challenged with P. gingivalis, compared with just 25% survival in control animals and just 5% survival in mice immunized with PgF-P8 only 21 days prior to challenge. Although the protection depended on the time of immunization before the bacterial challenge, it did not correlate with in vivo local cytokine production (IL-2, IL-4, IL-6, tumor necrosis factor alpha, and gamma interferon), specific antibody levels, or the isotype of anti-PgF-P8 antibodies produced.
N2W5
Creators: name: Alyssah Clavien
Year: 2023
Subjects: subject: Mesoamerica
valueUri: https://core.tdar.org/browse/geographic-keyword/61/mesoamerica
subject: Basin of Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/3/basin-of-mexico
subject: Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/134/mexico
subject: Mexico (State / Territory)
valueUri: https://core.tdar.org/browse/geographic-keyword/326/mexico-state-territory
subject: United Mexican States (Country)
valueUri: https://core.tdar.org/browse/geographic-keyword/438/united-mexican-states-country
subject: Central Mexico, Teotihuacan Valley
valueUri: https://core.tdar.org/browse/geographic-keyword/7/central-mexico-teotihuacan-valley
subject: Central Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/135/central-mexico
subject: Teotihuacan Valley
valueUri: https://core.tdar.org/browse/geographic-keyword/154/teotihuacan-valley
subject: Teotihuacan
valueUri: https://core.tdar.org/browse/geographic-keyword/202/teotihuacan
subject: Valley of Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/44/valley-of-mexico
subject: Classic Period
valueUri: https://core.tdar.org/browse/temporal-keyword/125/classic-period
subject: Terminal Formative
valueUri: https://core.tdar.org/browse/temporal-keyword/67/terminal-formative
subject: Teotihuacan Period
valueUri: https://core.tdar.org/browse/temporal-keyword/7765/teotihuacan-period
subject: Early Classic
valueUri: https://core.tdar.org/browse/temporal-keyword/5560/early-classic
subject: Teotihuacan
valueUri: https://core.tdar.org/browse/culture-keyword/7527/teotihuacan
subject: Teotihuacano
valueUri: https://core.tdar.org/browse/culture-keyword/9224/teotihuacano
subject: Teotihuacan
valueUri: https://core.tdar.org/browse/site-name/92/teotihuacan
subject: Town / City
valueUri: https://core.tdar.org/browse/site-type/255/town-city
subject: Domestic Structure or Architectural Complex
valueUri: https://core.tdar.org/browse/site-type/251/domestic-structure-or-architectural-complex
subject: Settlements
valueUri: https://core.tdar.org/browse/site-type/252/settlements
subject: Systematic Survey
valueUri: https://core.tdar.org/browse/investigation-type/12/systematic-survey

description: Site Survey Form Scan for Square N2W5.