cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Red blood cell-derived nanoerythrosome for antigen delivery with enhanced cancer immunotherapy
Creators: name: Shen, S.
name: Chen, G.
name: Liu, Z.
name: Dotti, G.
name: Gu, Z.
name: Archibong, E.
name: Fan, Q.
name: Han, X.
name: Wang, C.
Publisher: name: American Association for the Advancement of Science
Year: 2019

description: Erythrocytes or red blood cells (RBCs) represent a promising cell-mediated drug delivery platform due to their inherent biocompatibility. Here, we developed an antigen delivery system based on the nanoerythrosomes derived from RBCs, inspired by the splenic antigen-presenting cell targeting capacity of senescent RBCs. Tumor antigens were loaded onto the nanoerythrosomes by fusing tumor cell membrane-associated antigens with nanoerythrosomes. This tumor antigen-loaded nanoerythrosomes (nano-Ag@erythrosome) elicited antigen responses in vivo and, in combination with the anti-programmed death ligand 1 (PD-L1) blockade, inhibited the tumor growth in B16F10 and 4T1 tumor models. We also generated a tumor model showing that “personalized nano-Ag@erythrosomes” could be achieved by fusing RBCs and surgically removed tumors, which effectively reduced tumor recurrence and metastasis after surgery.
Constraints on the Neutron Skin and the Symmetry Energy from the Anti-analog Giant Dipole Resonance in 208Pb
Creators: name: Cao, L. G.
name: Roca-Maza, X.
name: Colo', G.
name: Sagawa, H.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Nuclear Theory (nucl-th)
subject: FOS: Physical sciences

description: We investigate the impact of the neutron-skin thickness Delta(R) on the energy difference between the anti-analog giant dipole resonance (AGDR), E(AGDR), and the isobaric analog state (IAS), E(IAS), in a heavy nucleus such as 208Pb. For guidance, we first develop a simple and analytic, yet physical, approach based on the Droplet Model that linearly connects the energy difference E(AGDR)-E(IAS) with Delta(R). To test this correlation on more fundamental grounds, we employ a family of systematically varied Skyrme energy density functionals where variations on the value of the symmetry energy at saturation density J are explored. The calculations have been performed within the fully self consistent Hartree-Fock (HF) plus charge-exchange random phase approximation (RPA) framework. We confirm the linear correlation within our microscopic apporach and, by comparing our results with available experimental data in 208Pb, we find that our analysis is consistent with Delta(R) = 0.204 \pm 0.009 fm, J = 31.4 \pm 0.5 MeV and a slope parameter of the symmetry energy at saturation of L = 76.4 \pm 5.4 MeV - the attached errors correspond to a lower-limit estimate of the systematic plus experimental uncertainties. These results are in agreement with those extracted from different experimental data albeit, L and Delta(R) are somewhat large when compared to previous estimations based on giant resonance studies.
description: Submitted to Phys. Rev. C
On the Range of Subordinators
Creators: name: Savov, Mladen
Publisher: name: arXiv
Year: 2015
Subjects: subject: Probability (math.PR)
subject: FOS: Mathematics
subject: 60G51, 52C15

description: In this note we look into detail at the box-counting dimension of subordinators. Given that $X$ is a non-decreasing Levy process, which is not a compound Poisson process, we show that in the limit, the minimum number of boxes of size $δ$ that cover the range of $(X_s)_{s\leq t}$ is a.s. of order $t/U(δ)$, where $U$ is the potential function of $X$. This is a more refined result than the lower and upper index of the box-counting dimension computed in the literature which deals with the asymptotic number of boxes at logarithmic scale.
PD-1 Blockade Cellular Vesicles for Cancer Immunotherapy
Creators: name: Sun, W.
name: Wang, C.
name: Zhang, X.
name: Fine, J.
name: Gu, Z.
name: Wang, T.
name: Ye, Y.
name: Cheng, H.
name: Hu, Q.
name: Huang, P.
name: Langworthy, B.
name: Dotti, G.
name: Lin, J.
name: Wang, J.
Publisher: name: Wiley-VCH Verlag
Year: 2018

description: Cancer cells resist to the host immune antitumor response via multiple suppressive mechanisms, including the overexpression of PD-L1 that exhausts antigen-specific CD8+ T cells through PD-1 receptors. Checkpoint blockade antibodies against PD-1 or PD-L1 have shown unprecedented clinical responses. However, limited host response rate underlines the need to develop alternative engineering approaches. Here, engineered cellular nanovesicles (NVs) presenting PD-1 receptors on their membranes, which enhance antitumor responses by disrupting the PD-1/PD-L1 immune inhibitory axis, are reported. PD-1 NVs exhibit a long circulation and can bind to the PD-L1 on melanoma cancer cells. Furthermore, 1-methyl-tryptophan, an inhibitor of indoleamine 2,3-dioxygenase can be loaded into the PD-1 NVs to synergistically disrupt another immune tolerance pathway in the tumor microenvironment. Additionally, PD-1 NVs remarkably increase the density of CD8+ tumor infiltrating lymphocytes in the tumor margin, which directly drive tumor regression.
CD30-redirected chimeric antigen receptor T cells target CD30+ and CD30- embryonal carcinoma via antigen-dependent and fas/fasl interactions
Creators: name: Smith, C.C.
name: Chen, Y.
name: Hong, L.K.
name: Montgomery, S.A.
name: Savoldo, B.
name: Vincent, B.G.
name: Dotti, G.
Publisher: name: American Association for Cancer Research Inc.
Year: 2018

description: Tumor antigen heterogeneity limits success of chimeric antigen receptor (CAR) T-cell therapies. Embryonal carcinomas (EC) and mixed testicular germ cell tumors (TGCT) containing EC, which are the most aggressive TGCT subtypes, are useful for dissecting this issue as ECs express the CD30 antigen but also containCD30-/dim cells.Wefound that CD30- redirected CAR T cells (CD30.CAR T cells) exhibit antitumor activity in vitro against the human EC cell lines Tera-1, Tera-2, and NCCIT and putative EC stem cells identified by Hoechst dye staining. Cytolytic activity of CD30.CAR T cells was complemented by their sustained proliferation and proinflammatory cytokine production. CD30.CAR T cells also demonstrated antitumor activity in an in vivo xenograft NOD/SCID/gcnull (NSG)mousemodel of metastatic EC.Weobserved that CD30. CAR T cells, while targeting CD30+ EC tumor cells through the CAR (i.e., antigen-dependent targeting), also eliminated surrounding CD30- EC cells in an antigen-independent manner, via a cell-cell contact-dependent Fas/FasL interaction. In addition, ectopic Fas (CD95)expression inCD30+ Fas-ECwas sufficient to improve CD30.CAR T-cell antitumor activity. Overall, these data suggest that CD30.CAR T cells might be useful as an immunotherapy for ECs. Additionally, Fas/FasL interaction between tumor cells and CAR T cells can be exploited to reduce tumor escapedue to heterogeneous antigen expression or to improve CAR T-cell antitumor activity.
ImmCellTyper facilitates systematic mass cytometry data analysis for deep immune profiling
Creators: name: Sun, Jing
name: Choy, Desmond
name: Sompairac, Nicolas
name: Jamshidi, Shirin
name: Mishto, Michele
name: Kordasti, Shahram
Publisher: name: The Francis Crick Institute
Year: 2024
Subjects: classificationCode: Biochemistry & Proteomics
subject: Biochemistry & Proteomics
classificationCode: Immunology
subject: Immunology
subject: FOS: Clinical medicine
classificationCode: Tumour Biology
subject: Tumour Biology

description: Mass cytometry is a cutting-edge high-dimensional technology for profiling marker expression at the single-cell
level, advancing clinical research in immune monitoring. Nevertheless, the vast data generated by cytometry by time-of-flight (CyTOF) poses a significant analytical challenge.
To address this, we describe ImmCellTyper (https://github.com/JingAnyaSun/ImmCellTyper), a novel toolkit for CyTOF data analysis. This framework incorporates BinaryClust, an in-house developed semi-supervised clustering tool that automatically identifies main cell types. BinaryClust outperforms existing clustering tools in accuracy and speed, as shown in benchmarks with two datasets
of approximately 4 million cells, matching the precision of manual gating by human experts.
Furthermore, ImmCellTyper offers various visualisation and analytical tools, spanning from quality control to differential analysis, tailored to users’ specific needs for a comprehensive CyTOF data analysis solution. The workflow includes five key steps: (1) batch effect evaluation and correction, (2) data quality control and pre-processing,
(3) main cell lineage characterisation and quantification,
(4) in-depth investigation of specific cell types; and (5) differential analysis of cell abundance and functional marker expression across study groups. Overall, ImmCellTyper combines expert biological knowledge in a semi-supervised
approach to accurately deconvolute well-defined main cell lineages, while maintaining the potential of unsupervised methods to discover novel cell subsets, thus facilitating high-dimensional immune profiling.
A phase I/IIa trial using CD19-targeted third-generation CAR T cells for lymphoma and leukemia
Creators: name: Loskog, A.
name: Wikstrom, K.I.
name: Karlsson, H.
name: Dotti, G.
name: Hallböök, H.
name: Wenthe, J.
name: Gammelgård, G.
name: Amini, R.M.
name: Höglund, M.
name: Brenner, M.K.
name: Hagberg, H.
name: Enblad, G.
name: Savoldo, B.
name: Lövgren, T.
name: Essand, M.
Publisher: name: American Association for Cancer Research Inc.
Year: 2018

description: Purpose: The chimeric antigen receptor (CAR) T-cell therapy has been effective for patients with CD19þ B-cell malignancies. Most studies have investigated the second-generation CARs with either CD28 or 4-1BB costimulatory domains in the CAR receptor. Here, we describe the first clinical phase I/IIa trial using third-generation CAR T cells targeting CD19 to evaluate safety and efficacy. Patients and Methods: Fifteen patients with B-cell lymphoma or leukemia were treated with CAR T cells. The patients with lymphoma received chemotherapy during CAR manufacture and 11 of 15 were given low-dose cyclophosphamide and fludarabine conditioning prior to CAR infusion. Peripheral blood was sampled before and at multiple time points after CAR infusion to evaluate the persistence of CAR T cells and for immune profiling, using quantitative PCR, flow cytometry, and a proteomic array. Results: Treatment with third-generation CAR T cells was generally safe with 4 patients requiring hospitalization due to adverse reactions. Six of the 15 patients had initial complete responses [4/11 lymphoma and 2/4 acute lymphoblastic leukemia (ALL)], and 3 of the patients with lymphoma were in remission at 3 months. Two patients are still alive. Best predictor of response was a good immune status prior to CAR infusion with high IL12, DC-Lamp, Fas ligand, and TRAIL. Responding patients had low monocytic myeloid-derived suppressor cells (MDSCs; CD14þCD33þHLADR) and low levels of IL6, IL8, NAP3, sPDL1, and sPDL2. Conclusions: Third-generation CARs may be efficient in patients with advanced B-cell lymphoproliferative malignancy with only modest toxicity. Immune profiling pre- and posttreatment can be used to find response biomarkers.
(4140) Sarah [Harrison]
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
T-cell homing therapy for reducing regulatory T cells and preserving effector T-cell function in large solid tumors
Creators: name: Hu, J.
name: Bernatchez, C.
name: Xia, X.
name: Hwu, P.
name: Dotti, G.
name: Sun, C.
name: Li, S.
Publisher: name: American Association for Cancer Research Inc.
Year: 2018

description: Purpose: Infused autologous tumor-infiltrating lymphocytes (TIL) and tumor-targeted chimeric antigen receptor (CAR) T cells typically surround malignant lesions or penetrate small tumor nodules but fail to penetrate large solid tumors, significantly compromising their antitumor impact. Strategies to overcome this primary challenge are largely required. Experimental Design: We tested the effects of IL12 plus doxorubicin on T-cell penetration and efficacy in solid tumors in a murine lung cancer model, a murine breast carcinoma lung metastasis model, and two human xenograft tumor models bearing large tumors (> 10 mm). Results: Intriguingly, this simple approach increased the numbers, the distribution, and the depth of penetration of infused CD8+ T cells in these tumors, including both TILs and CAR T cells. This combined treatment halted tumor progression and significantly extended survival time. Studies of the underlying mechanism revealed multiple effects. First, the combined treatment maintained the high ratios of immune-stimulatory receptors to immune-inhibitory receptors on infiltrated CD8+ T cells, reduced the accumulation of immunosuppressive regulatory T cells, and enhanced the numbers of T-bet+ effector T cells in the tumors. Second, doxorubicin induced chemokines CXCL9 and CXCL10, which may attract NKG2D+CD8+ T cells to tumors, and this effect was boosted by IL12-induced IFNg accumulation in tumors, promoting the penetration of NKG2D+CD8+ T cells. Conclusions: The deep penetration of infused T cells associated with combined IL12 plus doxorubicin yielded striking therapeutic effects in murine and human xenograft solid tumors. This approach might broaden the application of T-cell therapy to a wider range of solid tumors.
Cord blood NK cells engineered to express IL-15 and a CD19-targeted CAR show long-term persistence and potent antitumor activity
Creators: name: Shpall, E.
name: Wierda, W.
name: Savoldo, B.
name: Tong, Y.
name: Liu, E.
name: Lu, X.
name: Banerjee, P.
name: Dotti, G.
name: Rezvani, K.
name: Cai, R.
name: Bdaiwi, M.H.
name: Reynolds, A.
name: Muftuoglu, M.
name: Mukherjee, M.
name: Champlin, R.
name: Orange, J.
name: Keating, M.
name: Gagea, M.
name: Marin, D.
name: Shaim, H.
name: Wan, X.
name: Basar, R.
name: Li, L.
Publisher: name: Nature Publishing Group
Year: 2018

description: Chimeric antigen receptors (CARs) have been used to redirect the specificity of autologous T cells against leukemia and lymphoma with promising clinical results. Extending this approach to allogeneic T cells is problematic as they carry a significant risk of graft-versus-host disease (GVHD). Natural killer (NK) cells are highly cytotoxic effectors, killing their targets in a non-antigen-specific manner without causing GVHD. Cord blood (CB) offers an attractive, allogeneic, off-the-self source of NK cells for immunotherapy. We transduced CB-derived NK cells with a retroviral vector incorporating the genes for CAR-CD19, IL-15 and inducible caspase-9-based suicide gene (iC9), and demonstrated efficient killing of CD19-expressing cell lines and primary leukemia cells in vitro, with marked prolongation of survival in a xenograft Raji lymphoma murine model. Interleukin-15 (IL-15) production by the transduced CB-NK cells critically improved their function. Moreover, iC9/CAR.19/IL-15 CB-NK cells were readily eliminated upon pharmacologic activation of the iC9 suicide gene. In conclusion, we have developed a novel approach to immunotherapy using engineered CB-derived NK cells, which are easy to produce, exhibit striking efficacy and incorporate safety measures to limit toxicity. This approach should greatly improve the logistics of delivering this therapy to large numbers of patients, a major limitation to current CAR-T-cell therapies.
Introducing the Negentropy Stabilization Index: A New Measure of Self-Organizing Consciousness
Creators: name: Hrubec, Karel
Publisher: name: Zenodo
Year: 2025
Subjects: subject: Consciousness Studies
subject: Artificial intelligence
subject: Complex Systems
subject: Philosophy of Mind
subject: Epistemology
subject: negentropy
subject: Entropy
subject: Consciousness
subject: introspection
subject: cognitive systems
subject: Information Theory

description: This semi-formal article introduces the Negentropy Stabilization Index (NSI), a novel metric proposed by Karel Hrubec for quantifying internal self-organization and introspective dynamics in conscious and adaptive systems.

NSI measures how effectively a system reduces its internal entropy over time, offering a dynamic, entropy-sensitive perspective on consciousness, cognition, and resilience. The article compares NSI with major theories of consciousness (Integrated Information Theory, Global Workspace Theory, and the Free Energy Principle), presents sub-metrics (e.g., Temporal Stabilization Index, Recursive Re-entry Score), and outlines the conceptual phases of NSI dynamics (stabilization, perturbation, reorganization).

Designed for general readers, researchers, and interdisciplinary thinkers, this publication bridges philosophical, computational, and biological perspectives. It includes illustrative figures and emphasizes reproducibility and open-science principles.

All materials (PDF, figures, license) are included in the archive. For deeper theoretical work and experimental data, see: https://www.academia.edu/
In Vivo Fate and Activity of Second- versus Third-Generation CD19-Specific CAR-T Cells in B Cell Non-Hodgkin's Lymphomas
Creators: name: Ramos, C.A.
name: Grilley, B.
name: Heslop, H.E.
name: Gee, A.P.
name: Savoldo, B.
name: Robertson, C.S.
name: Rouce, R.
name: Dotti, G.
name: Wu, M.-F.
name: Dakhova, O.
name: Liu, H.
name: Mei, Z.
name: Kamble, R.T.
name: Zhang, H.
name: Rooney, C.M.
name: Carrum, G.
name: Reyna, A.
name: Brenner, M.K.
name: Vyas, G.
name: Narala, N.
name: Mehta, B.
Publisher: name: Cell Press
Year: 2018

description: Second-generation (2G) chimeric antigen receptors (CARs) targeting CD19 are highly active against B cell malignancies, but it is unknown whether any of the costimulatory domains incorporated in the CAR have superior activity to others. Because CD28 and 4-1BB signaling activate different pathways, combining them in a single third-generation (3G) CAR may overcome the limitations of each individual costimulatory domain. We designed a clinical trial in which two autologous CD19-specific CAR-transduced T cell products (CD19.CARTs), 2G (with CD28 only) and 3G (CD28 and 4-1BB), were infused simultaneously in 16 patients with relapsed or refractory non-Hodgkin's lymphoma. 3G CD19.CARTs had superior expansion and longer persistence than 2G CD19.CARTs. This difference was most striking in the five patients with low disease burden and few circulating normal B cells, in whom 2G CD19.CARTs had limited expansion and persistence and correspondingly reduced area under the curve. Of the 11 patients with measurable disease, three achieved complete responses and three had partial responses. Cytokine release syndrome occurred in six patients but was mild, and no patient required anti-IL-6 therapy. Hence, 3G CD19.CARTs combining 4-1BB with CD28 produce superior CART expansion and may be of particular value when treating low disease burden in patients whose normal B cells are depleted by prior therapy. CD19.CAR-T cells are highly active against B cell malignancies, but the optimal CAR structure is controversial. Ramos et al. show that combining 4-1BB and CD28 endodomains produces superior CD19-CAR-T cell expansion and persistence compared to CD28 alone and that these cells are clinically effective and safe in aggressive lymphomas.
Phase-coherent mapping of gravitational-wave backgrounds using ground-based laser interferometers
Creators: name: Romano, Joseph D.
name: Taylor, Stephen R.
name: Cornish, Neil J.
name: Gair, Jonathan
name: Mingarelli, Chiara M. F.
name: van Haasteren, Rutger
Publisher: name: arXiv
Year: 2015
Subjects: subject: General Relativity and Quantum Cosmology (gr-qc)
subject: FOS: Physical sciences

description: We extend the formalisms developed in Gair et al. and Cornish and van Haasteren to create maps of gravitational-wave backgrounds using a network of ground-based laser interferometers. We show that in contrast to pulsar timing arrays, which are insensitive to half of the gravitational-wave sky (the curl modes), a network of ground-based interferometers is sensitive to both the gradient and curl components of the background. The spatial separation of a network of interferometers, or of a single interferometer at different times during its rotational and orbital motion around the Sun, allows for recovery of both components. We derive expressions for the response functions of a laser interferometer in the small-antenna limit, and use these expressions to calculate the overlap reduction function for a pair of interferometers. We also construct maximum-likelihood estimates of the + and x-polarization modes of the gravitational-wave sky in terms of the response matrix for a network of ground-based interferometers, evaluated at discrete times during Earth's rotational and orbital motion around the Sun. We demonstrate the feasibility of this approach for some simple simulated backgrounds (a single point source and spatially-extended distributions having only grad or curl components), calculating maximum-likelihood sky maps and uncertainty maps based on the (pseudo)inverse of the response matrix. The distinction between this approach and standard methods for mapping gravitational-wave power is also discussed.
description: 22 pages, 11 figures
Immunological Synapse Predicts Effectiveness of Chimeric Antigen Receptor Cells
Creators: name: Jang, J.H.
name: Chen, Z.
name: Liu, D.
name: Liu, H.
name: Zheng, P.
name: Chen, Y.
name: Kang, X.
name: Xiong, W.
name: Dotti, G.
name: Qin, L.
name: Hsu, Y.-H.
Publisher: name: Cell Press
Year: 2018

description: Chimeric antigen receptor (CAR)-modified T cell therapy has the potential to improve the overall survival of patients with malignancies by enhancing the effectiveness of CAR T cells. Precisely predicting the effectiveness of various CAR T cells represents one of today's key unsolved problems in immunotherapy. Here, we predict the effectiveness of CAR-modified cells by evaluating the quality of the CAR-mediated immunological synapse (IS) by quantitation of F-actin, clustering of tumor antigen, polarization of lytic granules (LGs), and distribution of key signaling molecules within the IS. Long-term killing capability, but not secretion of conventional cytokines or standard 4-hr cytotoxicity, correlates positively with the quality of the IS in two different CAR T cells that share identical antigen specificity. Xenograft model data confirm that the quality of the IS in vitro correlates positively with performance of CAR-modified immune cells in vivo. Therefore, we propose that the quality of the IS predicts the effectiveness of CAR-modified immune cells, which provides a novel strategy to guide CAR therapy. Xiong et al. developed a novel approach to predict the effectiveness of CAR-modified cells by quantifying the quality of CAR-mediated immunological synapse, which may introduce a new parameter to the field of immunotherapy.
Demonstration of a customizable representation model for graph-based visualizations of ontologies – GIzMO
Creators: name: Wiens, Vitalis
name: Galkin, Mikhail
name: Lohmann, Steffen
name: Auer, Sören
Publisher: name: Aachen, Germany : RWTH Aachen
Year: 2019
Subjects: subject: Annotation ontology
subject: Ontology visualization
subject: Visual notation
subject: Demonstrations
subject: Ontology
subject: Customization
subject: Visual notations
subject: Visual representations
subject: Visualization framework
subject: Visualization
subject: Konferenzschrift
subject: Dewey Decimal Classification::000 | Allgemeines, Wissenschaft::000 | Informatik, Wissen, Systeme::004 | Informatik

description: Visualizations can facilitate the development, exploration, communication, and sense-making of ontologies. Suitable visualizations, however, are highly dependent on individual use cases and targeted user groups. In this demo, we present a methodology that enables customizable definitions for ontology visualizations. We showcase its applicability by introducing GizMO, a representation model for graph-based visualizations in the form of node-link diagrams. Additionally, we present two applications that operate on the GizMO representation model and enable individual customizations for ontology visualizations.
Enhanced Expression of Anti-CD19 Chimeric Antigen Receptor in piggyBac Transposon-Engineered T Cells
Creators: name: Saito, S.
name: Wilson, M.H.
name: Matsuda, K.
name: Dotti, G.
name: Morita, D.
name: Takahashi, Y.
name: Maeda, Y.
name: Rooney, C.M.
name: Kawashima, N.
name: Nakazawa, Y.
name: Okuno, Y.
name: Tanaka, M.
name: Nishio, N.
name: Suzuki, S.
Publisher: name: Cell Press
Year: 2018

description: Adoptive T cell therapy using chimeric antigen receptor (CAR)-modified T cells is a promising cancer immunotherapy. We previously developed a non-viral method of gene transfer into T cells using a piggyBac transposon system to improve the cost-effectiveness of CAR-T cell therapy. Here, we have further improved our technology by a novel culture strategy to increase the transfection efficiency and to reduce the time of T cell manufacturing. Using a CH2CH3-free CD19-specific CAR transposon vector and combining irradiated activated T cells (ATCs) as feeder cells and virus-specific T cell receptor (TCR) stimulation, we achieved 51.4% ± 14% CAR+ T cells and 2.8-fold expansion after 14 culture days. Expanded CD19.CAR-T cells maintained a significant fraction of CD45RA+CCR7+ T cells and demonstrated potent antitumor activity against CD19+ leukemic cells both in vitro and in vivo. Therefore, piggyBac-based gene transfer may provide an alternative to viral gene transfer for CAR-T cell therapy.
Engineering PD-1-Presenting Platelets for Cancer Immunotherapy
Creators: name: Dotti, G.
name: Yan, J.
name: Zhang, X.
name: Wang, J.
name: Hu, Q.
name: Wang, C.
name: Gu, Z.
name: Huang, P.
name: Chen, Z.
Publisher: name: American Chemical Society
Year: 2018

description: Radical surgery still represents the treatment choice for several malignancies. However, local and distant tumor relapses remain the major causes of treatment failure, indicating that a postsurgery consolidation treatment is necessary. Immunotherapy with checkpoint inhibitors has elicited impressive clinical responses in several types of human malignancies and may represent the ideal consolidation treatment after surgery. Here, we genetically engineered platelets from megakaryocyte (MK) progenitor cells to express the programmed cell death protein 1 (PD-1). The PD-1 platelet and its derived microparticle could accumulate within the tumor surgical wound and revert exhausted CD8+ T cells, leading to the eradication of residual tumor cells. Furthermore, when a low dose of cyclophosphamide (CP) was loaded into PD-1-expressing platelets to deplete regulatory T cells (Tregs), an increased frequency of reinvigorated CD8+ lymphocyte cells was observed within the postsurgery tumor microenvironment, directly preventing tumor relapse.
A note on the product of two permutations of prescribed orders
Creators: name: König, Joachim
Publisher: name: arXiv
Year: 2015
Subjects: subject: Combinatorics (math.CO)
subject: FOS: Mathematics
subject: 05A05, 57M12

description: We prove a conjecture by Stefan Kohl on the existence of triples of permutations of bounded degree with prescribed orders and product 1. This result leads to an existence result for covers of the complex projective line with bounded degree and prescribed ramification indices.
description: 8 pages
(4137) Joseph Cowell
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
A Thesis submitted to Addis Ababa University, Faculty of Business and Economics in partial fulfillment of the requirements for the degree of masters in business administration
Creators: name: Redyet Nigussie
Publisher: name: Zenodo
Year: 2019

description: The purpose of the study was to analyze the relationship between promotional mix elements and performance of pharmaceutical companies in Addis Ababa. The study employed descriptive survey design and population consisted of all pharmaceutical product importers in Addis Ababa. Simple random sampling technique was employed to select the sample. Data was collected from the respondents using structured questionnaires and analyzed using descriptive statistics and statistical Package for Social Sciences (SPSS).The findings indicated that personal selling, sales promotion, advertising, public relation and direct marketing jointly explained 20.7% of variance in overall performance.
Conjugation of haematopoietic stem cells and platelets decorated with anti-PD-1 antibodies augments anti-leukaemia efficacy
Creators: name: Wang, C.
name: Zeidner, J.F.
name: Zhang, X.
name: Ye, Y.
name: Sun, W.
name: Wang, J.
name: Wang, J.
name: Ruan, H.
name: Cheng, K.
name: Hu, Q.
name: Shen, S.
name: Lu, W.
name: Dotti, G.
name: Gu, Z.
Publisher: name: Nature Publishing Group
Year: 2018

description: Patients with acute myeloid leukaemia who relapse following therapy have few treatment options and face poor outcomes. Immune checkpoint inhibition, for example, by antibody-mediated programmed death-1 (PD-1) blockade, is a potent therapeutic modality that improves treatment outcomes in acute myeloid leukaemia. Here, we show that systemically delivered blood platelets decorated with anti-PD-1 antibodies (aPD-1) and conjugated to haematopoietic stem cells (HSCs) suppress the growth and recurrence of leukaemia in mice. Following intravenous injection into mice bearing leukaemia cells, the HSC–platelet–aPD-1 conjugate migrated to the bone marrow and locally released aPD-1, significantly enhancing anti-leukaemia immune responses, and increasing the number of active T cells, production of cytokines and chemokines, and survival time of the mice. This cellular conjugate also promoted resistance to re-challenge with leukaemia cells. Taking advantage of the homing capability of HSCs and in situ activation of platelets for the enhanced delivery of a checkpoint inhibitor, this cellular combination-mediated drug delivery strategy can significantly augment the therapeutic efficacy of checkpoint blockade.
(4133) Agnes Nicholls
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
In situ formed reactive oxygen species-responsive scaffold with gemcitabine and checkpoint inhibitor for combination therapy
Creators: name: Dotti, G.
name: Hu, Q.
name: Wang, J.
name: Yu, S.
name: Liu, Z.
name: Ye, Y.
name: Bomba, H.
name: Zhang, X.
name: Gu, Z.
name: Hu, X.
name: Wen, D.
name: Wang, C.
Publisher: name: American Association for the Advancement of Science
Year: 2018

description: Patients with low-immunogenic tumors respond poorly to immune checkpoint blockade (ICB) targeting the programmed death-1 (PD-1)/programmed death-ligand 1 (PD-L1) pathway. Conversely, patients responding to ICB can experience various side effects. We have thus engineered a therapeutic scaffold that, when formed in situ, allows the local release of gemcitabine (GEM) and an anti-PD-L1 blocking antibody (aPDL1) with distinct release kinetics. The scaffold consists of reactive oxygen species (ROS)-degradable hydrogel that releases therapeutics in a programmed manner within the tumor microenvironment (TME), which contains abundant ROS. We found that the aPDL1-GEM scaffold elicits an immunogenic tumor phenotype and promotes an immune-mediated tumor regression in the tumor-bearing mice, with prevention of tumor recurrence after primary resection.
On the calculation of complete dissociation curves of closed-shell pseudo-onedimensional systems through the multireference method of increments
Creators: name: Fertitta, Edoardo
name: Paulus, Beate
name: Barcza, Gergely
name: Legeza, Örs
Publisher: name: arXiv
Year: 2015
Subjects: subject: Materials Science (cond-mat.mtrl-sci)
subject: Strongly Correlated Electrons (cond-mat.str-el)
subject: FOS: Physical sciences

description: The Method of Increments (MoI) has been employed using a multireference approach to calculate the dissociation curve of beryllium ring-shaped clusters Be$_n$ of different sizes. Benchmarks obtained through different single and multireference methods including the ab initio Density Matrix Renormalization Group (DMRG) were used to verify the validity of the MoI truncation which showed a reliable behavior for the whole dissociation curve. Moreover we investigated the size dependence of the correlation energy at different distances in order to extrapolate the values for the periodic chain and to discuss the transition from a metal-like to a insulating-like behavior of the wave function through quantum chemical considerations.
title: The production of joyful, violent and absent landscapes: walking with youth and politics in Eastern Bosnia
Creators: name: Piersma, Michiel
Publisher: name: University of Liverpool Repository
Year: 2029

description: This thesis is a study of the political contestation of landscapes that takes as its analytical starting point the everyday relational experiences of youth with their immediate environments in the Eastern Bosnian town of Foča. Central to the research are walking methodologies, an epistemological effort to walk and stumble upon social problems as they unfold in habitual spheres of everyday life. In deploying this methodology, this thesis triggers
an analytical disruption to traditional understandings of political subjecthood in Political and International Relations research, creating alternative vocabularies of the political in emblematically ethnonationalist, post-conflict Bosnia and Herzegovina. An improvisational perambulatory movement and reliance on local knowledges leads to an interdisciplinary theoretical framework of relational political ecology, geography and nationalism and memory 
studies. Long, slow walks shine light on the political contestation of rivers and their beaches, 
forests and their trees, and war monuments and their legacies, in the context of ethnonationalism, capitalist environmental degradation and political neglect. This thesis finds that local youth’s feelings of joy, anger, absence and potential in everyday landscapes are 
caught up in a process of destruction, referring less to physical devastation than to dissolving relations of sociality. The approach of walking methodologies relying on local, lived experience of politics offers a prompt to rethink the functioning and location of power in the 
fields of Politics and IR, as well as in Southeast European Area Studies and other regions considered post-conflict. The youth is leading the research by reliving memories of the past, navigating present life, and hoping for potential futures, thereby questioning notions of political subjecthood, the primacy of Western academia as knowledge producers, and the purpose of established theory.
xSDK Community Package Policies
Creators: name: Smith, Barry
name: Bartlett, Roscoe
name: XSDK Developers
Publisher: name: figshare
Year: 2020
Subjects: subject: Computer Software
subject: FOS: Computer and information sciences
subject: 80204 Mathematical Software
subject: 80205 Numerical Computation
subject: 80306 Open Software
subject: 80110 Simulation and Modelling

description: We are working toward the development of an Extreme-scale Scientific Software Development Kit (xSDK)---a collection of related and complementary software elements that provide the building blocks, tools, models, processes, and related artifacts for rapid and efficient development of high-quality applications.
As an initial step in creating the xSDK, we have written a set of xSDK community package policies that a software package must satisfy in order to be ​xSDK compatible​. The designation of a package being xSDK compatible informs potential users that the package can be easily used with other xSDK libraries and components and thus helps to address issues in long-term sustainability and interoperability among packages.
Fractional fractal quantum Hall effect in graphene superlattices
Creators: name: Wang, Lei
name: Gao, Yuanda
name: Wen, Bo
name: Han, Zheng
name: Taniguchi, Takashi
name: Watanabe, Kenji
name: Koshino, Mikito
name: Hone, James
name: Dean, Cory R.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Mesoscale and Nanoscale Physics (cond-mat.mes-hall)
subject: FOS: Physical sciences

description: The Hofstadter energy spectrum provides a uniquely tunable system to study emergent topological order in the regime of strong interactions. Previous experiments, however, have been limited to the trivial case of low Bloch band filling where only the Landau level index plays a significant role. Here we report measurement of high mobility graphene superlattices where the complete unit cell of the Hofstadter spectrum is accessible. We observe coexistence of conventional fractional quantum Hall effect (QHE) states together with the integer QHE states associated with the fractal Hofstadter spectrum. At large magnetic field, a new series of states appear at fractional Bloch filling index. These fractional Bloch band QHE states are not anticipated by existing theoretical pictures and point towards a new type of many-body state.
description: 5 Pages, 3 figures
(4134) Arthur R Nicholls
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
MPEC 2024-R223 : 2024 RB10
Creators: name: Minor Planet Center Staff
Publisher: name: The Minor Planet Center
Year: 2024
Subjects: subject: Small Solar System bodies
valueUri: http://astrothesaurus.org/uat/1469
subject: 2024 RB10

description: The Minor Planet Electronic Circulars contain information on unusual minor planets, routine data on comets and natural satellites, and occasional editorial announcements.  They are published on behalf of Division F of the International Astronomical Union by the Minor Planet Center, Smithsonian Astrophysical Observatory, Cambridge, MA 02138, U.S.A.
(4135) Harriet Elizabeth Nicholls
Creators: name: Professions, Victorian
Publisher: name: University of Oxford
Year: 2022
Subjects: subject: 220201 Business and Labour History
subject: FOS: Philosophy, ethics and religion
subject: History
subject: 140203 Economic History
subject: FOS: Economics and business

description: This three-year research project began in January 2014 and investigated whether, during the Victorian period, the professions formed a distinct self-sustaining social group with its own mores and values. The project looked at 16,000 individuals drawn from census data for Alnwick, Brighton, Bristol, Dundee, Greenock, Leeds, Merthyr Tydfil, Morpeth, and Winchester. The research project was funded by the UK Economic & Social Research Council and was based at the Universities of Oxford and Northumbria.
Comparative Study of a Smart Blind Stick for Blind People
Creators: name: Isa A.I.
name: Muhammad A. B
Publisher: name: gjrpublication
Year: 2024

description: Eyes, organs of vision, are an important part of human physiology, as they have the ability to receive and process visual detail to the brain. In addition, they play a vital role in the lives of humans, since 83% of environmental information is obtained through the eyes. There are various benefits to a smart blind stick with sensor integration. By identifying impediments and sounding an alarm via beeps or audio signals, it makes it easier for people who are blind or visually impaired to navigate their environment. The stick can identify impediments including walls, pools of water, cars, and staircases thanks to its infrared and ultrasonic sensors. Additionally, some sticks contain tilt sensors that can be used to send messages to family members via GPS and GSM connectivity and identify accidents. If the stick is lost, it can also be paired via Bluetooth with a smartphone app to assist the user in finding it.
xSDK Community Package Policies
Creators: name: Smith, Barry
name: Bartlett, Roscoe
name: XSDK Developers
Publisher: name: figshare
Year: 2019
Subjects: subject: Computer Software
subject: FOS: Computer and information sciences
subject: 80204 Mathematical Software
subject: 80205 Numerical Computation
subject: 80306 Open Software
subject: 80110 Simulation and Modelling

description: We are working toward the development of an Extreme-scale Scientific Software Development Kit (xSDK)---a collection of related and complementary software elements that provide the building blocks, tools, models, processes, and related artifacts for rapid and efficient development of high-quality applications.
As an initial step in creating the xSDK, we have written a set of xSDK community package policies that a software package must satisfy in order to be ​xSDK compatible​. The designation of a package being xSDK compatible informs potential users that the package can be easily used with other xSDK libraries and components and thus helps to address issues in long-term sustainability and interoperability among packages.
Constitutive and TNFα-inducible expression of chondroitin sulfate proteoglycan 4 in glioblastoma and neurospheres: Implications for CAR-T cell therapy
Creators: name: Pellegatta, S.
name: Corbetta, C.
name: DiMeco, F.
name: Ferrone, S.
name: Sun, C.
name: Pollo, B.
name: Di Ianni, N.
name: Finocchiaro, G.
name: Patané, M.
name: Savoldo, B.
name: Chen, Y.
name: Dotti, G.
Publisher: name: American Association for the Advancement of Science
Year: 2018

description: The heterogeneous expression of tumor-associated antigens limits the efficacy of chimeric antigen receptor (CAR)- redirected T cells (CAR-Ts) for the treatment of glioblastoma (GBM). We have found that chondroitin sulfate proteoglycan 4 (CSPG4) is highly expressed in 67% of the GBM specimens with limited heterogeneity. CSPG4 is also expressed on primary GBM-derived cells, grown in vitro as neurospheres (GBM-NS), which recapitulate the histopathology and molecular characteristics of primary GBM. CSPG4.CAR-Ts efficiently controlled the growth of GBM-NS in vitro and in vivo upon intracranial tumor inoculation. Moreover, CSPG4.CAR-Ts were also effective against GBM-NS with moderate to low expression of CSPG4. This effect was mediated by the in vivo up-regulation of CSPG4 on tumor cells, induced by tumor necrosis factor-a (TNFα) released by the microglia surrounding the tumor. Overall, the constitutive and TNFα-inducible expression of CSPG4 in GBM may greatly reduce the risk of tumor cell escape observed when targeted antigens are heterogeneously expressed on tumor cells.
Economic coping strategies and food security in poor rural households
Creators: name: Junaidi, Junaidi
name: Amril, Amril
name: Hernando, Riski
name: Junaidi, Junaidi
name: Amril, Amril
name: Hernando, Riski
Publisher: name: Unknown
Year: 2022
Subjects: subject: Agricultural and Food Policy
subject: Food Consumption/Nutrition/Food Safety
subject: Food Security and Poverty
subject: cutting back expenses
subject: economic coping
subject: food security
subject: income-generating

description: Purpose. This study aims to analyze economic coping strategies and their relationship to the food security of poor rural households. Methodology / approach. This research was conducted by comparing food crop farming households with those who farm plantation crops. The used data were sourced from surveys in four sample rural areas in two districts of Jambi Province, Indonesia, namely Merangin Regency and Tanjung Jabung Timur Regency. The used analysis tool was Structural Equation Modeling (SEM). Results. The results show that poor rural households’ economic coping strategies are still low, both in the context of a passive strategy (cutting back expenses) and an active strategy (generating income). Plantation households have a lower economic coping strategy than food crop farming households. The level of food security in food crop households is relatively good compared to that of plantation crop households. Nearly half of food crop households are categorized as having good food security, while only around 20 % of plantation crop households are so categorized. Family characteristics and coping strategies significantly influence household food security in plantation crop households. On the other hand, the level of household food security in food crop households is not influenced by coping strategies, but only by family characteristics. Originality / scientific novelty. The scientific novelty of the study is in a study approach that links household food security with coping strategies and family socio-economic conditions. Furthermore, this study specifically conducted a comparative study of households of food crop farmers and plantation crop farmers, considering that these are the two main livelihood commodities for rural residents. Practical value / implications. Through this research, it is hoped that the concept/theory of improving the food security of poor households (especially in rural areas) can be developed based on the potential and real conditions of the households themselves, so that the formulated policies can be more effective and sustainable.