title: ÐÐ°ÑÑ-ÑÐ¿ÐµÐºÑÑÐ¾Ð¼ÐµÑÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð°Ð½Ð°Ð»Ð¸Ð· Ð¸Ð·Ð¾ÑÐ¾Ð¿Ð½Ð¾Ð³Ð¾ ÑÐ¾ÑÑÐ°Ð²Ð° ÑÐ³Ð»ÐµÑÐ¾Ð´Ð° 13Ð¡/12Ð¡ ÑÐ°ÑÑÐ¸ÑÐµÐ»ÑÐ½ÑÑ Ð¾Ð±ÑÐµÐºÑÐ¾Ð² Ð¿ÑÐ¸ Ð¸Ñ Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð´ÑÐ¾Ð¶Ð¶Ð°Ð¼Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Mass spectrometric analysis of the 13C/12C carbon isotope composition of plant objects during their metabolisation by yeast
Creators: name: ÐÐ»Ð¸ÑÐµÐµÐ², ÐÑÑÐµÐ½Ð¸Ð¹
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð¸Ð·Ð¾ÑÐ¾Ð¿Ñ ÑÐ³Ð»ÐµÑÐ¾Ð´Ð°
Saccharomyces cerevisiae
Ð¼Ð°ÑÑ-ÑÐ¿ÐµÐºÑÑÐ¾Ð¼ÐµÑÑÐ¸Ñ
ÑÐ¿ÐµÐºÑÑÐ°Ð»ÑÐ½ÑÐ¹ ÑÐ¾ÑÑÐ°Ð² ÑÐ²ÐµÑÐ°
ÑÐ¾ÑÐ¾ÑÐ¸Ð½ÑÐµÐ·
carbon isotopes
mass spectrometry
light spectral composition
photosynthesis

description: ÐÐ±ÑÐµÐºÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â ÑÐ°ÑÑÐµÐ½Ð¸Ñ Ñ C3-, C4- Ð¿ÑÑÑÐ¼Ð¸ ÑÐ¾ÑÐ¾ÑÐ¸Ð½ÑÐµÐ·Ð°. ÐÑÐµÐ´Ð¼ÐµÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â Ð¼Ð°ÑÑ-ÑÐ¿ÐµÐºÑÑÐ¾Ð¼ÐµÑÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð°Ð½Ð°Ð»Ð¸Ð· Ð³Ð°Ð·Ð¾Ð²ÑÑ ÑÐ°Ð· Ñ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸ÐµÐ¼ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¼ÐµÑÐ¾Ð´Ð° Ð¿ÑÐ¾Ð±Ð¾Ð¿Ð¾Ð´Ð³Ð¾ÑÐ¾Ð²ÐºÐ¸, Ð² ÑÐ¾Ð¼ ÑÐ¸ÑÐ»Ðµ Ð¿ÑÐ¸ ÑÐ¾ÑÐ¾ÑÐ¸Ð½ÑÐµÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÑÐ°ÑÑÐ¸ÑÐµÐ»ÑÐ½ÑÑ Ð¾Ð±ÑÐ°Ð·ÑÐ¾Ð². ÐÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ Ð¿ÑÐ¸ÐºÐ»Ð°Ð´Ð½ÑÐµ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ñ â Exel, Residual Gas Analyzer, Educio, Ð¥ÑÐ¾Ð¼Ð°ÑÐµÐº Ð°Ð½Ð°Ð»Ð¸ÑÐ¸Ðº. ÐÑÐµÐ´Ð»Ð¾Ð¶ÐµÐ½ Ð¸ Ð°Ð¿ÑÐ¾Ð±Ð¸ÑÐ¾Ð²Ð°Ð½ Ð¼ÐµÑÐ¾Ð´ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¿Ð¾Ð´Ð³Ð¾ÑÐ¾Ð²ÐºÐ¸ Ð¿ÑÐ¾Ð±, Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÑÑÐ¸Ð¹ Ð´ÐµÑÐµÐºÑÐ¸ÑÐ¾Ð²Ð°ÑÑ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ Ð¸Ð·Ð¾ÑÐ¾Ð¿Ð½Ð¾Ð³Ð¾ Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ñ ÑÐ³Ð»ÐµÑÐ¾Ð´Ð° 13Ð¡/12Ð¡ Ð¿ÑÐ¸ Ð¾Ð±Ð»ÑÑÐµÐ½Ð¸Ð¸ ÑÐ°ÑÑÐµÐ½Ð¸Ð¹ Ñ C3-, C4- Ð¿ÑÑÑÐ¼Ð¸ ÑÐ¸ÐºÑÐ°ÑÐ¸Ð¸ ÑÐ³Ð»ÐµÑÐ¾Ð´Ð° ÑÐ²ÐµÑÐ¾Ð¼ ÑÐ°Ð·Ð»Ð¸ÑÐ½Ð¾Ð³Ð¾ ÑÐ¿ÐµÐºÑÑÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÐ¾ÑÑÐ°Ð²Ð°: ÑÐ¸Ð½Ð¸Ð¼, ÐºÑÐ°ÑÐ½ÑÐ¼, Ð·ÐµÐ»ÐµÐ½ÑÐ¼ Ð¸ Ð±ÐµÐ»ÑÐ¼. ÐÑÐ¸ ÑÑÐ¾Ð¼ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð¿ÑÐ¾Ð±Ð»ÐµÐ¼Ñ Ð¿ÑÐ¸Ð¼ÐµÐ½Ð¸Ð¼Ð¾ÑÑÐ¸ Ð´Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð¼ÐµÑÐ¾Ð´Ð°: Ð¿Ð¾Ð´Ð±Ð¾Ñ Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼Ð¾Ð¹ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ Ð´Ð¸Ð¾ÐºÑÐ¸Ð´Ð° ÑÐ³Ð»ÐµÑÐ¾Ð´Ð°, ÐºÐ¾ÑÐ¾ÑÐ°Ñ Ð¿Ð¾Ð´ÑÐ¾Ð´Ð¸Ñ Ð´Ð»Ñ Ð¸Ð·Ð¾ÑÐ¾Ð¿Ð½Ð¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð°, Ð° ÑÐ°ÐºÐ¶Ðµ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ñ Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸ÑÐ¾Ð², Ð¾Ð±ÑÐ°Ð·ÑÑÑÐ¸ÑÑÑ Ð²ÑÐ»ÐµÐ´ÑÑÐ²Ð¸Ðµ Ð°Ð½Ð°ÑÑÐ¾Ð±Ð½Ð¾Ð³Ð¾ Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¼Ð° Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹, ÐºÐ¾ÑÐ¾ÑÐ°Ñ Ð¼Ð¾Ð¶ÐµÑ Ð²Ð»Ð¸ÑÑÑ Ð½Ð° Ð¸Ð·Ð¾ÑÐ¾Ð¿Ð½Ð¾Ðµ ÑÐ¾Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ðµ 13C/12C.
description: The object of research â plants with C3-, C4- photosynthesis pathways. The subject of the study - mass spectrometric analysis of gas phases using biological method of sample preparation, including photosynthetic activity of plant samples. The application programs â Exel, Residual Gas Analyzer, Educio, Chromatec Analyst. The biological sample preparation method was proposed and tested to detect changes in isotopic composition when photosynthesising plants are irradiated with light of different spectral composition: blue, red, green and white. The problems of applicability of this method were solved: the necessary concentration of carbon dioxide, which is suitable for isotopic analysis, as well as the concentration of metabolites formed due to anaerobic metabolism of yeast, which can affect the isotopic ratio 13C/12C.