title: Ð¡Ð¾Ð·Ð´Ð°Ð½Ð¸Ðµ Ð¸ Ð²Ð°Ð»Ð¸Ð´Ð°ÑÐ¸Ñ Ð¼Ð¾Ð´ÐµÐ»Ð¸ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð³ÑÐ¸Ð¿Ð¿Ð¾Ð·Ð½Ð¾Ð¹ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸ Ð¼ÑÑÐµÐ¹ Ð´Ð»Ñ ÑÐµÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÐºÐ°Ð½Ð´Ð¸Ð´Ð°ÑÐ½ÑÑ Ð¿ÑÐ¾ÑÐ¸Ð²Ð¾Ð³ÑÐ¸Ð¿Ð¿Ð¾Ð·Ð½ÑÑ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð²
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Development and validation of a mouse model of experimental influenza infection for testing the efficacy of candidate anti-influenza drugs
Creators: name: ÐÐ¸ÐºÑÑÐµÐ²Ð°, ÐÐ¸Ð»ÐµÐ½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð³ÑÐ¸Ð¿Ð¿
Ð¿ÑÐ¾ÑÐ¸Ð²Ð¾Ð³ÑÐ¸Ð¿Ð¿Ð¾Ð·Ð½ÑÐµ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÑ
Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ½ÑÐµ Ð¼ÑÑÐ¸
Ð¼Ð¾Ð´ÐµÐ»Ñ Ð³ÑÐ¸Ð¿Ð¿Ð¾Ð·Ð½Ð¾Ð¹ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸
influenza
anti-influenza drugs
laboratory mice
model of influenza infection

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description: The aim of this study is to develop a mouse model of experimental influenza infection using the A/California/07/09 H1N1pdm influenza virus strain. The object of the study was the A/California/07/09 H1N1pdm influenza virus strain. The subject of the study was a mouse model of influenza infection. The following tasks were accomplished in this study: 1. A review of the scientific literature on influenza infection, anti-influenza therapy, and methods for testing anti-influenza drugs was conducted. 2. The effect of body weight and age of white outbred mice on the dynamics of the infectious process during infection with the A/California/07/09 H1N1pdm influenza virus was studied. 3. The 50% lethal dose (LD50) of the A/California/07/09 H1N1pdm influenza virus for white outbred mice was determined. 4. The dynamics of the accumulation of influenza virus in the lung tissue of infected animals was evaluated. 5. The antiviral activity of the drugs TriazavirinÂ® and TamifluÂ® was studied in a mouse model of influenza pneumonia. The literature review was based on the following databases: eLIBRARY.RU; Scopus; CyberLeninka, "Students Consultant" EBS. Statistical processing of experimental data was carried out using the Microsoft ExÑel and GraphPad Prism 8.0 software packages. The following methods were used in the study: determination of the 50% lethal dose (LD50) of the influenza virus, titration of the virus in lung tissue, cellular enzyme-linked immunosorbent assay, determination of the efficacy of drugs in a mouse model of influenza infection, methods of statistical analysis of experimental results. The studies were conducted at the State Institute for Experimental Military Medicine of the Ministry of Defense of the Russian Federation in the virology laboratory. As a result of the study, a mouse model of experimental influenza infection was developed for planning and conducting experiments to assess the efficacy of antiviral drugs using the A/California/07/09 H1N1pdm influenza virus strain. Optimal parameters for this model were identified: white outbred mice weighing 12-15 grams and aged approximately 1.5 months, 50% lethal dose (LD50) of the influenza virus 6lg EID50/50 Âµl. The effectiveness of this model was confirmed by studies on the assessment of the antiviral activity of the drugs TriazavirinÂ® and TamifluÂ®.