cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
From Lagrangian to Quantum Mechanics with Symmetries
Creators: name: Nucci, M. C.
Publisher: name: arXiv
Year: 2012
Subjects: subject: Mathematical Physics (math-ph)
subject: Quantum Physics (quant-ph)
subject: FOS: Physical sciences

description: We present an old and regretfully forgotten method by Jacobi which allows one to find many Lagrangians of simple classical models and also of nonconservative systems. We underline that the knowledge of Lie symmetries generates Jacobi last multipliers and each of the latter yields a Lagrangian. Then it is shown that Noether's theorem can identify among those Lagrangians the physical Lagrangian(s) that will successfully lead to quantization. The preservation of the Noether symmetries as Lie symmetries of the corresponding Schrödinger equation is the key that takes classical mechanics into quantum mechanics. Some examples are presented.
description: To appear in: Proceedings of Symmetries in Science XV, Journal of Physics: Conference Series, (2012)
Similarities in Populations of Star Clusters
Creators: name: Fall, S. Michael
name: Chandar, Rupali
Publisher: name: arXiv
Year: 2012
Subjects: subject: Astrophysics of Galaxies (astro-ph.GA)
subject: FOS: Physical sciences

description: We compare the observed mass functions and age distributions of star clusters in six well-studied galaxies: the Milky Way, Magellanic Clouds, M83, M51, and Antennae. In combination, these distributions span wide ranges of mass and age: $10^2\lea M/M_{\odot}\lea10^6$ and $10^6\leaτ/yr \lea10^9$. We confirm that the distributions are well represented by power laws: $dN/dM\propto M^β$ with $β\approx-1.9$ and $dN/dτ\proptoτ^γ$ with $γ\approx -0.8$. The mass and age distributions are approximately independent of each other, ruling out simple models of mass-dependent disruption. As expected, there are minor differences among the exponents, at a level close to the true uncertainties, $ε_β\simε_γ\sim$~0.1--0.2. However, the overwhelming impression is the similarity of the mass functions and age distributions of clusters in these different galaxies, including giant and dwarf, quiescent and interacting galaxies. This is an important empirical result, justifying terms such as "universal" or "quasi-universal." We provide a partial theoretical explanation for these observations in terms of physical processes operating during the formation and disruption of the clusters, including star formation and feedback, subsequent stellar mass loss, and tidal interactions with passing molecular clouds. A full explanation will require additional information about the molecular clumps and star clusters in galaxies beyond the Milky Way.
description: 20 pages, 4 figures, 2 tables; published in the Astrophysical Journal, 752:96 (2012 June 20)
Density profile of water confined in cylindrical pores in MCM-41 silica
Creators: name: Soper, Alan K
Publisher: name: arXiv
Year: 2011
Subjects: subject: Disordered Systems and Neural Networks (cond-mat.dis-nn)
subject: FOS: Physical sciences

description: It is shown that traditional estimates of the pore diameter in the porous silica material MCM-41-S15 (of order 15Å) are too small to allow the amount of water that is absorbed by these materials (around 0.5gH2O/g substrate) to occur only inside the pore. Either the additional water is absorbed on the surface of the silica particles and outside the pores, or else the pores are larger than the traditional estimates. In addition the low Q Bragg intensities from a sample of MCM-41-S15 porous silica under different dry and wet conditions and with different hydrogen isotopes are simulated using a simple model of the water and silica density profile across the pore. It is found the best agreement of these intensities with experimental data is shown by assuming the much larger pore diameter of 25Å(radius 12.5Å). Qualitative agreement is found between these simulated density profiles and those found in recent empirical potential structure refinement simulations of the same data, even though the latter data did not specifically include the Bragg peaks in the structure refinement. It is shown that the change in the (100) peak intensity on cooling from 300K to 210K, which previously has been ascribed to a change in density of the confined water on cooling, can equally be ascribed to a change in density profile at constant average density. It is further pointed out that, independent of whether the pore diameter really is as large as 25Å or whether a significant amount of water is absorbed outside the pore, the earlier reports of a dynamic cross-over in supercooled confined water could in fact be a crystallisation transition in the larger pore or surface water.
description: 20 pages, 8 figures
Poliakoff's Breaking the silence: a director's study for production
Creators: name: Hemphill, Geraldine Anne
Publisher: name: University of Calgary
Year: 1990
Subjects: subject: PR 6066 O4 Z684 1990
subject: Poliakoff, Stephen, 1952- Breaking the silence
subject: Theater - Production and direction

description: Bibliography: p. 176-179.
Identifying A Protein Biomarker In Blood Applying An Unbiased Data-Driven Approach
Creators: name: Staubli, Frank
Publisher: name: Zenodo
Year: 2015
Subjects: Biomarkers
transcriptomics
gene expression
data-driven
curated data

description: regulation profiles. To start with, we used GENEVESTIGATOR(1) to identify genes strongly and specifically expressed in chosen cancer types (as compared to normal tissues and over 1,000 other cancer types). The search was performed across 40'000 curated Affymetrix expression arrays covering a wide variety of well-described experiments and resulted in up to 20 marker genes highly specific for a particular cancer type. The corresponding proteins were then filtered by molecular properties, leading to a few testable candidates expected to be abundant, cancer specific, and secreted into blood. This method was successfully applied to a chosen cancer type as demonstrated by experimental validation. Out of four candidate proteins tested with ELISA assays from blood samples of cancer patients, one showed significant discriminative power.
Our method can similarly be used to identify drug targets by applying protein filters for druggability, cellular localization or molecular function. The power of this approach is the very large number of experimental conditions that can be simultaneously screened to find genes with very specific profiles, combined with protein property data. It offers an unbiased, data-driven and global context-based approach for selecting testable candidates.
Rethinking Closed-loop Training for Autonomous Driving
Creators: name: Zhang, Chris
name: Guo, Runsheng
name: Zeng, Wenyuan
name: Xiong, Yuwen
name: Dai, Binbin
name: Hu, Rui
name: Ren, Mengye
name: Urtasun, Raquel
Publisher: name: arXiv
Year: 2023
Subjects: subject: Robotics (cs.RO)
subject: Computer Vision and Pattern Recognition (cs.CV)
subject: Machine Learning (cs.LG)
subject: FOS: Computer and information sciences

description: Recent advances in high-fidelity simulators have enabled closed-loop training of autonomous driving agents, potentially solving the distribution shift in training v.s. deployment and allowing training to be scaled both safely and cheaply. However, there is a lack of understanding of how to build effective training benchmarks for closed-loop training. In this work, we present the first empirical study which analyzes the effects of different training benchmark designs on the success of learning agents, such as how to design traffic scenarios and scale training environments. Furthermore, we show that many popular RL algorithms cannot achieve satisfactory performance in the context of autonomous driving, as they lack long-term planning and take an extremely long time to train. To address these issues, we propose trajectory value learning (TRAVL), an RL-based driving agent that performs planning with multistep look-ahead and exploits cheaply generated imagined data for efficient learning. Our experiments show that TRAVL can learn much faster and produce safer maneuvers compared to all the baselines. For more information, visit the project website: https://waabi.ai/research/travl
description: ECCV 2022
Williams Committee Surveys: National Educational Survey, 1977; National Survey of Post Secondary Teaching Staff, 1977
Creators: name: Beed, T. W.
Publisher: name: UK Data Service
Year: 1980

description: The surveys were commissioned by the Committee of Inquiry into Education and Training, set up by the Australian Government, in order to gather information from students and staff in all sectors of Australian post-secondary education. Areas covered include characteristics of students and staff, career choice, preparation and planning and attitudes towards issues of importance to post-secondary education in the seventies.
Robustness and observability of rotating vortex-lattices in an exciton-polariton condensate
Creators: name: Borgh, Magnus O.
name: Franchetti, Guido
name: Keeling, Jonathan
name: Berloff, Natalia G.
Publisher: name: arXiv
Year: 2012
Subjects: subject: Quantum Gases (cond-mat.quant-gas)
subject: FOS: Physical sciences

description: Exciton-polariton condensates display a variety of intriguing pattern-forming behaviors, particularly when confined in potential traps. It has previously been predicted that triangular lattices of vortices of the same sign will form spontaneously as the result of surface instabilities in a harmonic trap. However, natural disorder, deviation of the external potential from circular symmetry, or higher-order terms modifying the dynamical equations may all have detrimental effects and destabilize the circular trajectories of vortices. Here we address these issues, by characterizing the robustness of the vortex lattice against disorder and deformations of the trapping potential. Since most experiments use time integrated measurements it would be hard to observe directly the rotating vortex lattices or distinguish them from vortex-free states. We suggest how these difficulties can be overcome and present an experimentally viable interference-imaging scheme that would allow the detection of rotating vortex lattices.
description: 12 pages, 12 figures
Grants or Loans? : Views of a Sample of Bath and Exeter University Students and Their Parents, 1978-1979
Creators: name: Sandford, C.
name: Lewis, A.
name: Thomson, N.
Publisher: name: UK Data Service
Year: 1981

description: To assess the views of a sample of university students and a sample of parents of university students towards the present scheme of financing students' maintenance costs (means-tested grant) as compared with alternative schemes (universal grants, loans, or loan/grant mixture).
A review of multi-scale barriers to transitioning from digital agriculture to a digital bioeconomy
Creators: name: Eastwood, Callum
name: Turner, James
name: Romera, Alvaro
name: Selbie, Diana
name: Henwood, Roxanne
name: Espig, Martin
name: Wever, Mark
Publisher: name: AgResearch
Year: 2023
Subjects: subject: Agriculture, land and farm management
subject: Environmental biotechnology
subject: FOS: Environmental biotechnology
subject: Bioremediation

description: The use of digital technologies in agriculture has received significant attention in the last decade. There is increasing interest in the potential opportunities for digitalization at a broader bioeconomy scale; however, there is limited knowledge of the potential barriers to a digital bioeconomy. This chapter reviews current knowledge on barriers to digital agriculture and uses a case study to relate these barriers to the bioeconomy scale. We found that adoption barriers are not just technical, but include economic, social, and institutional dimensions, and occur at multiple scales involving technology design, farm systems (including supply chains), the agricultural innovation system, and society. Additionally, these barriers can be highly interconnected. For example technical issues around data interoperability cannot be addressed independently of social issues at the farm scale related to perceptions around privacy and transparent use of farmer data. Examining these multi-dimensional and multi-scale issues through a bioeconomy lens highlights the need for directionality in digital bioeconomy innovation and alignment of national policies and initiatives. Rather than assuming that greater use of digital tools is inherently positive for a national bioeconomy, nations should purposely assess and anticipate the potential implications of digitalization. Our review highlights three opportunities for directionality in the digital bioeconomy. The first is for technology design and development to directly respond to and address societal (not only end-user) needs and barriers to uptake. The second is to design and develop data governance, business models, and standards for data, which are transparent, inspire trust, and share benefits of digital technologies among supply chain stakeholders. The third is to considerably broaden the assessment of societal value from digital agriculture. Addressing the adoption barriers to the digital bioeconomy will come from integrated applications of digitalization that are purpose or ‘mission’ led, rather than inherently techno-centric. NZBIDA
Identifying A Protein Biomarker In Blood Applying An Unbiased Data-Driven Approach
Creators: name: Staubli, Frank
Publisher: name: Zenodo
Year: 2015
Subjects: Biomarker
transcriptomics
data-driven
curation

description: The search for protein biomarkers in blood often involves a pre-selection of potential candidates based on literature, followed by an experimental validation of these proteins in a given experimental or clinical context. The success rate of this approach is often low because a) the upfront choice of candidates is biased towards previously studied proteins, b) these proteins are often not specific enough, and c) the number of screened proteins is limited. By contrast, a huge number of gene expression experiments have been published, providing a valuable information source for a huge variety of experimental contexts. However, it is crucial to curate and structure the data prior to integrating it into a searchable platform and thus being able to efficiently use it. The GENEVESTIGATOR platform turns out to be an extremely powerful tools to support our search for proteins strongly over-expressed in cancers and secreted into the bloodstream. We first identified genes strongly and specifically expressed in chosen cancer types (as compared to normal tissues and over 1,000 other cancer types). The search was performed across 40'000 curated Affymetrix expression arrays covering a wide variety of well-described experiments. It resulted in sets of up to 20 marker genes being highly specific for a particular cancer type. The corresponding proteins were then filtered by molecular properties, resulting in a few individual testable candidates expected to be abundant, cancer specific, and secreted to the blood. The method was successfully applied to a chosen cancer type as demonstrated by experimental validation. In fact, from four candidate proteins tested by ELISA assays in protein extracts from blood samples of cancer patients, one protein was well detected and showed significant discriminative power. The method could similarly also be used to identify drug targets after applying protein filters for druggability, cellular localization or molecular function. The power of this approach is the very large number of experimental conditions that can be simultaneously screened to find genes with an extremely specific profile, combined with protein property data. As opposed to literature search, it offers an unbiased, data-driven and global context-based approach for selecting testable candidates.
One step beyond: The excursion set approach with correlated steps
Creators: name: Musso, Marcello
name: Sheth, Ravi K.
Publisher: name: arXiv
Year: 2012
Subjects: subject: Cosmology and Nongalactic Astrophysics (astro-ph.CO)
subject: High Energy Physics - Theory (hep-th)
subject: FOS: Physical sciences

description: We provide a simple formula that accurately approximates the first crossing distribution of barriers having a wide variety of shapes, by random walks with a wide range of correlations between steps. Special cases of it are useful for estimating halo abundances, evolution, and bias, as well as the nonlinear counts in cells distribution. We discuss how it can be extended to allow for the dependence of the barrier on quantities other than overdensity, to construct an excursion set model for peaks, and to show why assembly and scale dependent bias are generic even at the linear level.
description: 5 pages, 1 figure. Uses mn2e class style
Professional Socialisation: a Case Study of Estate Management Students, 1975-1980
Creators: name: Joseph, M.
Publisher: name: UK Data Service
Year: 1986

description: The purpose of this study was to define the content of group culture, and to outline the socialisation process by which it is acquired amongst students on a three-year surveying course at a polytecnic. This involved ascertaining whether there is something which could be called the culture of the surveying profession, or at least the culture of the students in that profession, and, if so, to identify and examine the component parts of this culture.
The Enhancement Of Peptide Drugs Entrapped In Nanovesicles
Creators: name: Panyosak, Tritsida
Publisher: name: Zenodo
Year: 2015
Subjects: peptide drugs
nanovesicles
liposomes
niosomes

description: This study was to develop the entrapment model of various charged peptide drugs (bacitracin, insulin and bovine serum albumin(BSA)) in nanovesicles (liposomes and niosomes).

Cationic, anionic and neutral liposomes were prepared from dipalmitoyl phosphatidylcholine (DPPC), cholesterol (CHL), dimethyldioctadecylammonium bromide (DDAB) and/or dicetylphosphate (DP), whereas cationic, anionic and neutral niosomes were composed of sorbitan monostearate (Span 60) or polyoxyethylene sorbitanmonostearate (Tween 61), CHL, DDAB and/or DP at various molar ratios. Morphology and vesicular sizes of the vesicles were investigated by optical microscope and dynamic light scattering (DLS), respectively. The entrapment efficiency of the peptides in the vesicles was determined by gelelectrophoresis and gel documentation. The prepared empty liposomes and niosomes were in white powder after lyophilization. They were multilamellar layers with the vesicular sizes ranging from 40-60 nm, except for the neutral niosomes entrapped with insulin and cationic niosomes entrapped with BSA showed the sizes ranging from 0.1-1.3 μm. and 100-150 nm, respectively. The entrapment efficiency of BSA, bacitracin and insulin in neutral niosomes (Tween61/CHL 1:1) was 72.9%, 69.9% and 10.3%, in cationic niosomes (Tween61/CHL/DDAB 1:1:0.05) was 84.5%, 32.8 and 87.2% and in anionic niosomes (Tween61/CHL/DP 1:1:0.05) was 50.1%, 90.9% and 44.3%, respectively.

This study has demonstrated the enhancement of entrapment efficiency of various peptide drugs by modifying the charges of the bilayer vesicles. The results from this study can suggest the proper model of nanovesicular formulation to entrap peptides with different charges and polarity for pharmaceutical and cosmeceutical application.
Exploring Framed Gauge Theory as Basis for Physical Models
Creators: name: Chan, H. M.
name: Tsou, S. T.
Publisher: name: arXiv
Year: 2011
Subjects: subject: High Energy Physics - Phenomenology (hep-ph)
subject: High Energy Physics - Theory (hep-th)
subject: FOS: Physical sciences

description: It is shown that by introducing as dynamical variables in the formulation of gauge theories the frame vectors (or vielbeins) in internal symmetry space, in addition to the standard gauge boson and matter fermion fields, one obtains: (i) for the $su(2) \times u(1)$ symmetry, the standard electroweak theory with the Higgs field thrown in as part of the framed gauge theoretical structure, (ii) for the $su(3) \times su(2) \times u(1)$ symmetry, a "framed standard model" with, apart from the Higgs field as before, a global $su(3)$ symmetry to play the role of fermion generations, plus some other properties which are shown elsewhere to give to both quarks and leptons hierarchical mass and mixing patterns similar to those experimentally observed. Besides, the "framing" of the standard model as such has brought the particle theory closer in structure to the theory of gravity where vierbeins have long figured as dynamical variables. Although most of the results have already been reported before, time and hindsight have allowed their presentation in this review to be made more transparent and succint.
description: 34 pages, Latex
Enumerators Returns for County Antrim, 1851
Creators: name: Macafee, W.
name: Morgan, V.
Publisher: name: UK Data Service
Year: 1981

description: The purpose of this study was to transcribe and code the surviving enumerators' returns from the Irish census of 1851, and to analyse this data in order to gain evidence about the demographic and social structure of parts of County Antrim during the mid-nineteenth century.
Optimal control strategies for tuberculosis treatment: a case study in Angola
Creators: name: Silva, Cristiana J.
name: Torres, Delfim F. M.
Publisher: name: arXiv
Year: 2012
Subjects: subject: Optimization and Control (math.OC)
subject: Populations and Evolution (q-bio.PE)
subject: FOS: Mathematics
subject: FOS: Biological sciences
subject: 92D30, 49M05

description: We apply optimal control theory to a tuberculosis model given by a system of ordinary differential equations. Optimal control strategies are proposed to minimize the cost of interventions. Numerical simulations are given using data from Angola.
description: This is a preprint of a paper whose final and definite form will appear in the international journal Numerical Algebra, Control and Optimization (NACO). Paper accepted for publication 15-March-2012
The enabling and constraining connections between trust and digitalisation in incumbent value chains
Creators: name: Rijswijk, Kelly
name: de Vries, Jasper
name: Klerkx, Laurens
name: Turner, James
Publisher: name: AgResearch
Year: 2023
Subjects: Technology management

description: Digitalisation is a disruptive socio-technical process that goes beyond digital technologies and their use within an organisation, and involves besides (in many cases radical) technological change, social, institutional and economic change. This creates uncertainties for value chain actors and the trust relationships between them. In this paper we aim to understand the connections between trust and digitalisation. We investigate how trust relations affect digitalisation, and how digitalisation affects trust relations among value chain actors, using the Dutch flower sector as a case study. Our findings show that the sector has a high level of interpersonal trust, but limited institutional trust, as the relationships between companies are highly competitive and transactional. In this context, limited trust hinders digitalisation in multiple and mutually reinforcing ways, inducing a vicious cycle whereby existing distrust or limited trust results in limited digitalisation, which in turn causes more distrust due to uncertainties around the digitalisation process, further increased by existing (technological) path dependencies. Hence there is a need for 1) awareness of mutually reinforcing (dis)trust dynamics and vicious (or virtuous) cycles in relation to digitalisation are needed; 2) higher levels of understanding of what digitalisation entails and 3) joint strategy building and foresighting in the value chain. NZBIDA
Investigation of the Novel Attributes of a Dual Material Gate Nanoscale Tunnel Field Effect Transistor
Creators: name: Saurabh, Sneh
name: Kumar, M. Jagadesh
Publisher: name: arXiv
Year: 2011
Subjects: subject: Mesoscale and Nanoscale Physics (cond-mat.mes-hall)
subject: FOS: Physical sciences

description: In this paper, we propose the application of a Dual Material Gate (DMG) in a Tunnel Field Effect Transistor (TFET) to simultaneously optimize the on-current, the off-current and the threshold voltage, and also improve the average subthreshold slope, the nature of the output characteristics and the immunity against the DIBL effects. We demonstrate that if appropriate work-functions are chosen for the gate materials on the source side and the drain side, the tunnel field effect transistor shows a significantly improved performance. We apply the technique of DMG in a Strained Double Gate Tunnel Field Effect Transistor with a high-k gate dielectric to show an overall improvement in the characteristics of the device along with achieving a good on-current and an excellent average subthreshold slope. The results show that the DMG technique can be applied to TFETs with different channel materials, channel lengths, gate-oxide materials, gate-oxide thicknesses and power supply levels to achieve significant gains in the overall device characteristics.
An Approach To Control Purity Of The Drug Substance For Non-Clinical Study
Creators: name: Okubo, Yusuke
Publisher: name: Zenodo
Year: 2015
Subjects: API
Process Chemistry
Impurity

description: Background:Purity of drug substance (API) used for clinical study must be the same orhigher than that of API used for non-clinical study (NCAPI). It is important toallow flexibility in developing manufacturing process to designand control the purity of NCAPI. There arevarious ways to optimize the purity on purpose, such as addition of individualimpurities on use of harsh chemical reactions.Inthe present study, we established a method to ensurethe desired purity of NCAPI by adding a separate setof API containing various species of impurities (IMAPI).
Methods: Firstly, the target purity ofNCAPI was determined by the purity of API used for a two-week repeated dosetoxicity study in the dog. Secondly, IMAPI was prepared in lab, and labexperiments were conducted to determine how much IMAPIneeded to achieve the target purity of NCAPI at large-scalemanufacturing. Thirdly, we conducted large-scale manufacturing by addition ofIMAPI and confirmed whether the purity of NCAPI was within the desired one.
Results: The purity of API used in the dogtoxicity study was 97.8%, so we set the target purity at 98%. IMAPI wassynthesized with the purity of 82.2%, and added to the reaction mixture inlarge-scale production. Crystallization and filtration gave 37.4kgofNCAPI with the purity of 99%, which is slightly higher than the target purity.
Conclusions:Theseresults indicate that this method is useful for purity design of NCAPI.
Super-renormalizable Multidimensional Quantum Gravity
Creators: name: Modesto, Leonardo
Publisher: name: arXiv
Year: 2012
Subjects: subject: High Energy Physics - Theory (hep-th)
subject: General Relativity and Quantum Cosmology (gr-qc)
subject: FOS: Physical sciences

description: In this paper we introduce a perturbatively super-renormalizable and unitary theory of quantum gravity in any dimension D. The theory presents two entire functions, a.k.a. "form factors", and a finite number of local operators required by the quantum consistency of the theory itself. The main reason to introduce the entire functions is to avoid ghosts (states of negative norm) like the one in the four-dimensional Stelle's theory. The new theory is indeed ghost-free since the two entire functions have the property to generalize the Einstein-Hilbert action without introducing new poles in the propagator. The theory is renormalizable at one loop and finite from two loops upward. In this paper we essentially study three classes of form factors, systematically showing the tree-level unitarity. We prove that the gravitation potential is regular in r = 0 for all the choices of form factors compatible with renormalizability and unitarity. We also include Black hole spherical symmetric solutions omitting higher curvature corrections to the equation of motions. For two out of three form factors the solutions are regular and the classical singularity is replaced by a "de Sitter-like core" in r=0. For one particular choice of the form factors, we prove that the D-dimensional "Newtonian cosmology" is singularity-free and the Universe spontaneously follows a de Sitter evolution at the "Planck scale" for any matter content. We conclude the article stating that, in the ultraviolet regime, the spectral dimension takes on different values for the three cases: less than or equal to "1" for the first case, "0" for the second one and "2" for the third one. Once the class of theories compatible with renormalizability and unitarity is defined, the spectral dimension has the same short-distance "critical value" or "accumulation point" for any value of the topological dimension D.
description: 20 pages, 13 figures. arXiv admin note: substantial text overlap with arXiv:1107.2403
Fluorescence-Based Assay Development To Screen Drugs Against Parkinson?S Disease
Creators: name: Salado, Clarisa
Publisher: name: Zenodo
Year: 2015
Subjects: Parkinson's
screening
parkin
recruitment

description: Parkinson?s disease (PD) involves the generation of dopaminergic (DA) neurons in the substantia nigra, causing tremor and motor impairment. Defects in PARK2 protein are the cause of Parkinson?s disease type 2 (PARK2), also known as early-onset parkinsonism with diurnal fluctuation (EPDF) or autosomal recessive juvenile Parkinson?s disease (PDJ). In mouse model, Parkin is critical for removing dysfunctional mitochondria via autophagy. The absence of Parkin does not affect turnover under normal conditions. However, during stress conditions, Parkin helps to remove damaged mitochondria by autophagy. Innoprot has developed a novel fluorescence cell-based assay for High Content Screening to screen compounds that can promote Parkin2 mithocondrial localization without mitochondrial stress induction. In this work, we used this model to screen a library of 1200 compounds. After the screening campaign, the positive compounds were chosen for further testing, based on the strength of the initial response and the lack of cytotoxicity. Our results indicate that Parkin2 cell-based assay is a valid strategy for drug screening.
Baculovirus Expression Vectors For Optimal Recombinant Protein And Virus-Like Particle Production
Creators: name: Chambers, Adam
name: Glanville, Ben De
name: King, Linda
name: Possee, Robert
Publisher: name: Zenodo
Year: 2015
Subjects: Baculovirus
protein production
virus like particles
flashBAC
OET

description: There is increasing demand for high quality, biologically active recombinant proteins in awide range of applications, from simple positive controls for immunoassays to complex virus like particles (VLPs) as sub unit vaccines. Baculoviruses have been employed for over 30 years to make recombinant proteins.  One of the most popular systems currently used to make recombinant baculoviruses is flashBAC.  This utilizes a one step process whereby a plasmid transfer vector with a foreign gene is mixed with flashBAC and used to cotransfect insect cells.  Five days later, a virus containing the gene is available for making recombinant protein to very high levels in virus-infected cell cultures.  This system has also been tailored to optimise the production of particular types of protein.  flashBACGOLD maximises production of membrane-bound (e.g. receptors) or secreted proteins (e.g. urokinase) owing to deletion of chitinase and cathepsin genes, whose products limit transport through the endoplasmic reticulum and promote protease activity.  Another variant, flashBACULTRA lacks the virus p10 gene as well as chitinase and cathepsin, which decreases virus-infected cell lysis in the later stages of infection and contamination of recombinant protein preparations with cellular debris.  Both of these flashBAC variants are ideal for producing VLPs (e.g. influenza virus) that bud from the plasma membrane.  Conversely, for VLPs that remain intracellular and are protease resistant, it can be advantageous to use flashBACPRIME, which retains chitinase, cathepsin and p10.  This ensures that the virus-infected cells lyse in the terminal stages of replication and release VLPs that are easy to purify.
Costa Rican Peasants, 1972-1973
Creators: name: Seligson, M. A.
Publisher: name: UK Data Service
Year: 1981

description: To measure several social-psychological, behavioural, and socio-economic attributes of smallholders and landless labourers in Costa Rica. The survey focused on those peasants engaged in agrarian capitalist production and hence avoided interviews with peasants whose primary means of production was subsistence farming.
A combinatorial characterization of tight fusion frames
Creators: name: Bownik, Marcin
name: Luoto, Kurt
name: Richmond, Edward
Publisher: name: arXiv
Year: 2011
Subjects: subject: Functional Analysis (math.FA)
subject: Combinatorics (math.CO)
subject: FOS: Mathematics
subject: 42C15, 15A57, 05E05

description: In this paper we give a combinatorial characterization of tight fusion frame (TFF) sequences using Littlewood-Richardson skew tableaux. The equal rank case has been solved recently by Casazza, Fickus, Mixon, Wang, and Zhou. Our characterization does not have this limitation. We also develop some methods for generating TFF sequences. The basic technique is a majorization principle for TFF sequences combined with spatial and Naimark dualities. We use these methods and our characterization to give necessary and sufficient conditions which are satisfied by the first three highest ranks. We also give a combinatorial interpretation of spatial and Naimark dualities in terms of Littlewood-Richardson coefficients. We exhibit four classes of TFF sequences which have unique maximal elements with respect to majorization partial order. Finally, we give several examples illustrating our techniques including an example of tight fusion frame which can not be constructed by the existing spectral tetris techniques. We end the paper by giving a complete list of maximal TFF sequences in dimensions less than ten.
description: 31 pages
Multicolor Fluorescent Biosensors For Multiplexed Detection Of Gpcr Receptor Activation
Creators: name: Ramos, Isbaal
Publisher: name: Zenodo
Year: 2015
Subjects: GPCR
Biosensor
receptor
internalization
multiplex assay

description: We describe a new live-cell assay that uses fluorescent biosensors co-expressed with several GPCRs for measuring the second messenger concentration changes that occurs after GPCR activation. The molecular structure of the Biosensors comprises: a membrane localization peptide, a second messenger transduction protein binding peptide, a reticulum retention signal and a fluorescent peptide. The biosensors are normally localized in the Plasma Membrane but an increase in the second messenger concentration leads to a change in the structural folding of the Biosensor that promotes its vesicularization. The second messenger transduction protein binding peptide could be replaced depending on the second messenger, resulting three different versions of the biosensors: cAMP; Calcium and DAG Nomad Biosensor. In addition, we also present here the development of multiplexed assays using the different versions of the Biosensor enabling the analysis both different intracellular signaling and receptor internalization, simultaneously.
Aircraft Noise and Sleep Disturbance, 1979
Creators: name: Civil Aviation Authority, Directorate Of Operational Research
name: Social And Community Planning Research
Publisher: name: UK Data Service
Year: 1981

description: The aim of this survey was to provide a scientific basis for deciding future policy on aircraft movements at night at Heathrow and Gatwick. The survey sought:
 a) to establish the nature and scale of sleep disturbance from all causes around Heathrow and Gatwick airports;
 b) to assess the significance of aircraft noise in causing sleep disturbance;
 c) to investigate the relationship between exposure to aircraft noise and the degree of sleep disturbance.
Monitoring Cell Health In Real-Time & In 3D
Creators: name: Edward, Roy
Publisher: name: Zenodo
Year: 2015
Subjects: Cell Health; HCS; spheroids; real-time; microscopy; flow cytometry; fluorescence; far-red;

description: A novel far-red DNA binding viability probe, DRAQ7, has undetectable toxicity in long-term / real-time cell based assays as validated in recent publications (Akagi et al., 2013, Marciniak et al., 2013, Wang et al., 2015 and internal data) including ultra-sensitive bioassays for DNA intercalators, ex-plant tissue culture and nano-particle toxicity, and importantly in the presence of toxicants / anti-cancer compounds.

Thus, cells can be exposed to it at any stage of an assay to permit real-time monitoring of loss of membrane integrity (in apoptosis, death). Being DNA specific it allows monitoring cell-by-cell while its spectral properties mean it can be incorporated into multi-colour experiments, or with Hoechst 33342 or CyTRAK Orange (for simple cell health assays) or with mitochondrial membrane potential probes such as TMRM.

DRAQ7 is truly cell impermeant yet retains the DNA targeting and far-red fluorescence of the parent DRAQ chromophore. Accordingly, it likewise can be used on HCS imaging platforms, fluorescence microscopes, cytometers and sorters. Its spectral properties (long wavelength) are particularly suited to penetrative imaging of multi-cellular structures and thick ex-plant tissue sections.

The presentation will focus on the deployment and application of DRAQ7 to demanding real-time and 3D micro-tissue assays including patient-derived samples that enable strategies for personalised medicine, underpinned by core performance data that define its unique properties.
The generator problem for Z-stable C*-algebras
Creators: name: Thiel, Hannes
name: Winter, Wilhelm
Publisher: name: arXiv
Year: 2012
Subjects: subject: Operator Algebras (math.OA)
subject: FOS: Mathematics
subject: 46L05, 46L85 (Primary) 46L35 (Secondary)

description: The generator problem was posed by Kadison in 1967, and it remains open until today. We provide a solution for the class of C*-algebras absorbing the Jiang-Su algebra Z tensorially. More precisely, we show that every unital, separable, Z-stable C*-algebras A is singly generated, which means that there exists an element x in A that is not contained in any proper sub-C*-algebra of A. To give applications of our result, we observe that Z can be embedded into the reduced group C*-algebra of a discrete group that contains a non-cyclic, free subgroup. It follows that certain tensor products with reduced group C*-algebras are singly generated. In particular, the tensor product of two reduced free group C*-algebras is singly generated.
description: 15 pages
Identification Of Differentially Expressed Micro- And Mrna Clusters In Blood Of Patients With Coronary Artery Disease: Intersection With Calcification Pathways And Implications In Cardio-Vascular Diseases
Creators: name: Mkrtchyan, Garik
name: Shindyapina, Anastasia
Publisher: name: Zenodo
Year: 2015
Subjects: coronary artery disease
calcification
microRNAs
signalling pathways
bioinformatics

description: Coronary artery disease (CAD) is one of the main and common types of cardio-vascular diseases, which occurs as a result of atherosclerotic plaque formation in blood vessels during ageing. Increased levels of calcium deposition were detected in blood vessels of patients with CAD and linked to early CAD formation. Here, we hypothesized that these two processes are linked and possess common molecular basis, which is realized through regulation of target genes involved in calcification and CAD.

To validate our hypothesis, we used a bioinformatics approach, in which micro- and mRNA data of blood samples from 12 patients with CAD were analyzed. Several high quality data sets were extracted from GEO database, analyzed by commercially available program TR GeneGo Metacore, statistically verified by J-express algorithms and analyzed using statistical methods implemented in R.

Analysis of GEO data revealed patterns of differentially expressed microRNAs in patients with CAD. We have shown the involvement of the differentially expressed microRNAs (hsa-miR-154; hsa-miR-451; hsa-miR-17) in signalling pathways associated with vascular calcification. Additionally, we obtained data of mRNA expression, showing several clusters of genes, which are involved in vascular calcification pathways in patients with CAD and developed an interaction map of differentially expressed genes in the blood of CAD patients and genes associated with vascular calcification.

We conclude that patients with CAD have several differentially expressed gene markers, which may have different impact on calcification development. Data provided may have a positive outcome for cardio-vascular diagnosis strategy and personalized medicine.
Use Of High Throughput Electric Field Stimulation (Efs) Coupled With Intracellular Ca2+ Kinetics Measurements On Ipsc-Derived Cardiomyocytes
Creators: name: D'Angelo, Jean Marc
Publisher: name: Zenodo
Year: 2015
Subjects: DRUG SAFETY

description: Hamamatsu has developed a 96-channel electrode array system that is mounted on the  FDSS/?CELL. It adds electric field stimulations (EFS) to all 96 wells in a microplate simultaneously while fluorescence/luminescence signals are monitored. We measured oscillations of intracellular Ca2+ concentration, which occurs along with the beating of the cells, with a calcium sensitive fluorescent dye in human iPSC-derived cardiomyocytes (Cor.4U, Axiogenesis AG). We observed that the Ca2+ oscillation in cardiomyocytes was synchronized to the electric stimulation, which indicates that the EFS system is able to pace the beating of cardiomyocytes. We also observed that the Ca2+ oscillations in the presence of some ion channel blockers were modulated by adding EFS. Use of EFS coupled with intracellular Ca2+ kinetics measurements, in the high-throughput manner, should be useful in in vitro assessment of cardiac toxicity of pharmacological compounds using cardiomyocytes, in particular in toxicity screening at early stages of drug development, as well as in cardiomyocyte research.
Computer Analysis by Multidimensional Scaling of House of Commons Division Lists, 1861
Creators: name: Cromwell, V.
name: Osmond, C.
Publisher: name: UK Data Service
Year: 1981

description: The purpose of this study was to test the usefulness and adaptability of multidimensional scaling by applying it to the analysis of Commons' Division Lists for one parliamentary session, 1861. An additional purpose was to test for groupings of M.P.s by forming maps of similarity of voting behaviour.
Hospitalised Children in Swansea, 1972
Creators: name: Stacey, M.
name: Earthrowl, B.
Publisher: name: UK Data Service
Year: 1981

description: The aim of this study was to investigate cultural factors often thought to be associated with social classes, leading to differences of attitude and belief and to variation in behaviour with regard to patterns of child rearing, and, more specifically, to investigate the proposal that working class parents do not understand the social and emotional needs of their children in hospital.
Ultrasolitons: multistability and subcritical power threshold from higher-order Kerr terms
Creators: name: Novoa, David
name: Tommasini, Daniele
name: Michinel, Humberto
Publisher: name: arXiv
Year: 2012
Subjects: subject: Pattern Formation and Solitons (nlin.PS)
subject: Optics (physics.optics)
subject: FOS: Physical sciences

description: We show that an optical system involving competing higher-order Kerr nonlinearities can support the existence of ultrasolitons, namely extremely localized modes that only appear above a certain threshold for the central intensity. Such new solitary waves can be produced for powers below the usual collapse threshold, but they can also coexist with ordinary, lower-intensity solitons. We derive analytical conditions for the occurrence of multistability and analyze the dynamics of the different kinds of fundamental eigenmodes that can be excited in these nonlinear systems. We also discuss the possible transitions between solitary waves belonging to different nonlinear regimes through the mechanism of soliton switching.
description: 5 pages, 4 figures, to appear in EPL
Optogenetics: A Bright Future For Voltage Gated Ion Channels
Creators: name: D'Angelo, Jean Marc
Publisher: name: Zenodo
Year: 2015
Subjects: Optogentics
Voltage gated ion channels
Channelrhodopsin-2 (ChR2)

description: Channels Channelrhodopsin-2 (ChR2) is a light-activated microbial cation channel which can be used to depolarize neurons through the incidence of blue light (470 nm).   The possibility to optically control the plasma membrane voltage opens new and interesting perspectives for the characterization of voltage-gated ion channels and the search for modulators.   Proof of principle studies have been performed to verify the applicability of this tool for the development of cell based assays in High Throughput Screening (HTS) platforms as microplate readers.An HEK-293 cell line, which stably co-expresses the human voltage-gated calcium channel hCav1.3 and the inward rectifier hKir2.3 channel, was over-transfected with a ChR2 carrying a single amino acids mutation.   This latter result in a prolonged lifetime of the conducting state of ChR2 and in a reduced light power requirement for its activation.  A protocol of light stimulation of ChR2 and record of calcium ion influx through Cav1.3 with the use of a calcium-sensitive fluorescent dye (Fluo8) was tested in the Hamamatsu FDSS?cell. A well-known Cav1.3 blocker, Isradipine, tested in the resting and the partial inactivated Cav1.3 states, was used to confirm the pharmacological profile.Data obtained for the ChR2/hCav1.3 cell line by light stimulation have been also compared to the extracellular potassium stimulus and to patchclamp to cross-check their reliability.
Wachter, R. (2019): Pompejanische Wandinschriften
Creators: name: Lobe, Michael
Publisher: name: Forum Classicum
Year: 2020

description: Rezension zu: Wachter, R. (2019): Pompejanische Wandinschriften. Sammlung Tusculum, Berlin/Boston, De Gruyter, 563 S. (ISBN: 978-3-11-064943-7)
description: Forum Classicum, Nr. 3 (2020): Forum Classicum
Unemployed Professionals and Executives, 1977
Creators: name: Berthoud, R. G.
Publisher: name: UK Data Service
Year: 1981

description: The aim of this survey was to investigate the labour market experiences of unemployed professionals and executives; to describe those who became unemployed and the circumstances surrounding their departure from their previous jobs; to examine their job seeking activities and experiences; to compare their success in finding new jobs, and to examine the role of Professional and Executive Recruitment (PER) in the context of the general picture.
Song, B. / Leidorf, K. / Heller, E. (2019): Luftbildarchäologie. Spuren der Vergangenheit aus der Luft. Methoden und Techniken – Klassisch und virtuell
Creators: name: Schmitz, Dietmar
Publisher: name: Forum Classicum
Year: 2020

description: Rezension zu: Song, B. / Leidorf, K. / Heller, E. (2019): Luftbildarchäologie. Spuren der Vergangenheit aus der Luft. Methoden und Techniken – Klassisch und virtuell, Darmstadt, 168 S. (ISBN 978-3-8062-3887-7)
description: Forum Classicum, Nr. 3 (2020): Forum Classicum