title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ ÑÑÐ¾Ð²Ð½ÐµÐ¹ ÑÐµÑÐ¼ÐµÐ½ÑÐ¾Ð² Ð² ÑÐ¸ÑÑÐµÐ¼Ðµ ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ CfrBI Ð½Ð° Ð·Ð°ÑÐ¸ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ Ð¾Ñ ÑÐ°Ð³Ð°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: The effect of enzyme levels in the CfrBI restriction-modification system on the protection of bacteria from phage
Creators: name: ÐÐ°Ð»Ð¸Ð½Ð½Ð¸ÐºÐ¾Ð²Ð°, ÐÑÐ±Ð¾Ð²Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð¡frBI
Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³
ÑÐ¸ÑÑÐµÐ¼Ñ ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸
ÑÐ¸ÑÑÐµÐ¼Ñ Ð·Ð°ÑÐ¸ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð¾Ñ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð¾Ð²
in vivo Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ
ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð°Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ
Escherichia coli
CfrBI
bacteriophage
restriction-modification systems
cell protection systems
in vivo research
fluorescence microscopy

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«ÐÐ»Ð¸ÑÐ½Ð¸Ðµ ÑÑÐ¾Ð²Ð½ÐµÐ¹ ÑÐµÑÐ¼ÐµÐ½ÑÐ¾Ð² Ð² ÑÐ¸ÑÑÐµÐ¼Ðµ ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ CfrBI Ð½Ð° Ð·Ð°ÑÐ¸ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ Ð¾Ñ ÑÐ°Ð³Ð°Â». ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ ÑÐ¸ÑÑÐµÐ¼Ñ Ð·Ð°ÑÐ¸ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ CfrBI Ð¾Ñ ÑÐ°Ð³Ð¾Ð²Ð¾Ð¹ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸. Ð¦ÐµÐ»ÑÑ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÐµÑÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ ÑÑÐ¾Ð²Ð½ÐµÐ¹ Ð±ÐµÐ»ÐºÐ¾Ð² Ð² ÑÐ¸ÑÑÐµÐ¼Ðµ ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ CfrBI Ð½Ð° Ð·Ð°ÑÐ¸ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ E. coli Ð¾Ñ ÑÐ°Ð³Ð° Ð»ÑÐ¼Ð±Ð´Ð°. ÐÐ»Ñ ÑÑÐ¾Ð³Ð¾ Ð² Ð¿ÐµÑÐ²Ð¾Ð¹ Ð³Ð»Ð°Ð²Ðµ Ð¾Ð¿Ð¸ÑÐ°Ð½Ñ Ð¼ÐµÑÐ¾Ð´Ñ Ð·Ð°ÑÐ¸ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ Ð¾Ñ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð¾Ð², ÑÐ°ÑÑÐ¼Ð¾ÑÑÐµÐ½Ñ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÐµ ÑÐ¸ÑÑÐµÐ¼Ñ ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸, ÑÐ¾Ð±ÑÐ°Ð½Ñ Ð´Ð°Ð½Ð½ÑÐµ Ð¾ ÑÐ¸ÑÑÐµÐ¼Ðµ CfrBI. ÐÐ¾ Ð²ÑÐ¾ÑÐ¾Ð¹ Ð³Ð»Ð°Ð²Ðµ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÐµÐ½Ñ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ñ Ð¸ Ð¼ÐµÑÐ¾Ð´Ñ, Ð¸ÑÐ¿Ð¾Ð»ÑÐ·ÑÐµÐ¼ÑÐµ Ð² ÐÐÐ . Ð ÑÑÐµÑÑÐµÐ¹ Ð³Ð»Ð°Ð²Ðµ Ð¸Ð·Ð»Ð¾Ð¶ÐµÐ½Ñ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÑÐ¾Ð²Ð½ÐµÐ¹ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ ÑÐµÑÐ¼ÐµÐ½ÑÐ¾Ð² ÑÐ¸ÑÑÐµÐ¼Ñ Ð Ð Ð² ÐºÐ»ÐµÑÐºÐ°Ñ Ð¸ Ð·Ð°ÑÐ°Ð¶ÐµÐ½Ð¸Ñ ÑÐ°Ð³Ð¾Ð¼ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ Ñ ÑÐ¸ÑÑÐµÐ¼Ð¾Ð¹ Ð¡frBI. ÐÑÐ¸Ð²ÐµÐ´ÐµÐ½Ñ ÑÐ¾Ð·Ð´Ð°Ð½Ð½ÑÐµ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ðµ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ð¸ Ð´Ð»Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð², ÐºÐ¾Ð´Ð¸ÑÑÑÑÐ¸Ðµ Ð±ÐµÐ»ÐºÐ¸ ÑÐ½Ð´Ð¾Ð½ÑÐºÐ»ÐµÐ°Ð·Ñ ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸ Ð¸ Ð¼ÐµÑÐ¸Ð»ÑÑÐ°Ð½ÑÑÐµÑÐ°Ð·Ñ, ÑÐ»Ð¸ÑÑÐµ Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½ÑÐ¼Ð¸ Ð±ÐµÐ»ÐºÐ°Ð¼Ð¸. ÐÐ»Ð°Ð²Ð½ÑÐµ Ð²ÑÐ²Ð¾Ð´Ñ ÑÐ°Ð±Ð¾ÑÑ: 1) ÐºÐ¾Ð¿Ð¸Ð¹Ð½Ð¾ÑÑÑ ÑÑÐ°Ð½ÑÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð¹ Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð´Ñ Ð² E. coli Ñ ÑÐ¸ÑÑÐµÐ¼Ð¾Ð¹ CfrBI Ð²Ð»Ð¸ÑÐµÑ Ð½Ð° ÑÑÐ¾Ð²ÐµÐ½Ñ Ð·Ð°ÑÐ¸ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ Ð¾Ñ Ð²Ð¸ÑÑÐ»ÐµÐ½ÑÐ½Ð¾Ð³Ð¾ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð° Ð»ÑÐ¼Ð±Ð´Ð°; 2) Ð¾Ñ ÐºÐ¾Ð¿Ð¸Ð¹Ð½Ð¾ÑÑÐ¸ ÑÑÐ°Ð½ÑÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð¹ Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð´Ñ Ð·Ð°Ð²Ð¸ÑÐ¸Ñ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð¾ ÑÐµÑÐ¼ÐµÐ½ÑÐ¾Ð² ÑÐ¸ÑÑÐµÐ¼Ñ CfrBI Ð¸ Ð¸Ñ ÑÐ¾Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ðµ; 3) Ð½Ð°Ð¸Ð»ÑÑÑÑÑ Ð·Ð°ÑÐ¸ÑÑ ÑÐ¸ÑÑÐµÐ¼Ð° CfrBI Ð² E. coli Ð´Ð°ÐµÑ, Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÐµÐ½Ð½Ð¾Ð¹ Ð² Ð²Ð¸Ð´Ðµ ÑÑÐµÐ´Ð½ÐµÐºÐ¾Ð¿Ð¸Ð¹Ð½Ð¾Ð¹ Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð´Ñ, Ð½Ð°Ð¸ÑÑÐ´ÑÑÑ â Ð²ÑÑÐ¾ÐºÐ¾ÐºÐ¾Ð¿Ð¸Ð¹Ð½Ð°ÑÑ. Ð¡ÑÐµÐ´Ð½ÑÑ Ð·Ð°ÑÐ¸ÑÑ Ð´Ð°ÐµÑ Ð½Ð¸Ð·ÐºÐ¾ÐºÐ¾Ð¿Ð¸Ð¹Ð½Ð°Ñ.
description: The subject of the graduate qualification work is "The effect of enzyme levels in the CfrBI restriction-modification system on the protection of bacteria from phage." The given work is devoted to the study of the CfrBI bacterial protection system against phage infection. The aim of this work is to study the effect of protein levels in the CfrBI restriction-modification system on the protection of E. coli bacteria from phage. To do this, the first chapter describes methods of protecting bacteria from bacteriophages, examines various restriction-modification systems, and collects data on the CfrBI system. The second chapter presents the materials and methods used in the WRC. The third chapter presents the results of a study of the levels of expression of enzymes of the RM system in cells and phage infection of bacteria with the Sakish system. The created genetic constructs for the expression of genes encoding restriction endonuclease and methyltransferase proteins fused with fluorescent proteins are presented. The main conclusions of the work: 1) the copyness of the transformed plasmid in E. coli with the CfrBI system affects the level of protection of bacteria from the virulent bacteriophage lambda; 2) the number of enzymes of the CfrBI system and their ratio depend on the copyness of the transformed plasmid; 3) the CfrBI system in E. coli provides the best protection, represented as a mediumâcopy plasmid, the worst - high-copy. The average protection is provided by a low copy.