Additional file 1 of Dual-dynamic-bond cross-linked injectable hydrogel of multifunction for intervertebral disc degeneration therapy
Creators: name: Yang, Linjun
name: Yu, Congcong
name: Fan, Xuhui
name: Zeng, Tianni
name: Yang, Wentao
name: Xia, Jiechao
name: Wang, Jianle
name: Yao, Litao
name: Hu, Chuan
name: Jin, Yang
name: Zhu, Yutao
name: Chen, Jiaxin
name: Hu, Zhijun
Publisher: name: figshare
Year: 2022
Subjects: subject: Biophysics
subject: Biochemistry
subject: Space Science
subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Infectious Diseases
subject: FOS: Health sciences

description: Additional file 1: Figure S1. Abridged general view of PBNPs formation. Figure S2. Dynamic light scattering (DLS) of the PBNPs. Figure S3. Thermogravimetry-differential scanning calorimetry (TG-DSC) analyses of the PBNPs. Figure S4. Adsorption isotherms of the PBNPs, demonstrating its mesoporous structure. Figure S5. Photographs of OBG hydrogel formation. Figure S6. SEM images of the OBG and PBNPs@OBG. Scale bar = 100 µm. Figure S7. EDS mapping images of the PBNPs@OBG. Scale bar = 100 µm. Figure S8. In vitro degradation percentages of the PBNPs@OBG in vitro, in PBS with different pH values of 7.4 and 6.5 at 37 °C. Figure S9. Photograph of the OBG and PBNPs@OBG in compression tests. Figure S10. A Weight lifting ability for the glass adhered by the PBNPs@OBG hydrogel. B Adhesiveness strengths of the OBG and PBNPs@OBG (n = 3, ns not significant). Figure S11. H2O2 decomposed into H2O and O2 in the presence of PBNPs within 10 min. Figure S12. JC-1 staining quantified as the ratios of Red/Green fluorescence intensities. (n = 3, **P &lt; 0.01 versus H2O2 alone.). Figure S13. A–B Effect of the PBNPs@OBG on NPs apoptosis after H2O2 treatment by annexin V-FITC and propidium iodide staining. (n = 3, **P &lt; 0.01, ***P &lt; 0.001). Figure S14. Geometrically optimized PBNPs observed from different angles. Figure S15. Charge density difference of different species adsorbed on the PBNPs substrates. The yellow color represents charge accumulation, while green color is the charge loses. Figure S16. Immunofluorescence staining of aggrecan, collagen II, MMP3, and MMP13 in NP tissues at 8 weeks after injection. Scale bar = 50 µm. Figure S17. A Living/dead staining images of NP cells in vitro. Scale bar = 200 µm. B CCK-8 assay of NP cells after being treated with different materials. Figure S18. Blood routine examination results of WBC at 4 weeks. 1. Control; 2. PBNPs@OBG; 3. OBG; 4. OBG; 5. Acupuncture (n = 3, ns not significant). Figure S19. Blood routine examination results of WBC at 8 weeks. 1. Control; 2. PBNPs@OBG; 3. OBG; 4. OBG; 5. Acupuncture (n = 3, ns not significant). Figure S20. Blood routine examination results of RBC at 4 weeks. 1. Control; 2. PBNPs@OBG; 3. OBG; 4. OBG; 5. Acupuncture (n = 3, ns not significant). Figure S21. Blood routine examination results of RBC at 8 weeks. 1. Control; 2. PBNPs@OBG; 3. OBG; 4. OBG; 5. Acupuncture (n = 3, ns not significant). Figure S22. Blood routine examination results of platelets at 4 weeks. 1. Control; 2. PBNPs@OBG; 3. OBG; 4. OBG; 5. Acupuncture (n = 3, ns not significant). Figure S23. Blood routine examination results of platelets at 8 weeks. 1. Control; 2. PBNPs@OBG; 3. OBG; 4. OBG; 5. Acupuncture (n = 3, ns: not significant). Figure S24. Standard blood biochemical examination at 4 weeks. 1. Control; 2. PBNPs@OBG; 3. OBG; 4. OBG; 5. Acupuncture (n = 3, **P &lt; 0.01, ns not significant). Figure S25. Standard blood biochemical examination at 8 weeks. 1. Control; 2. PBNPs@OBG; 3. OBG; 4. OBG; 5. Acupuncture (n = 3, ns not significant). Figure S26. H&amp;E staining of heart, liver, spleen, lung, and kidney at 4 weeks after operation. Scale bar = 100 µm. Figure S27. Histological screening at 8 weeks after operation. Scale bar = 100 µm. Table S1. Primer used for Qpcr.