title: ÐÐ¿ÑÐ¸Ð¼Ð¸Ð·Ð°ÑÐ¸Ñ ÑÐ¾ÑÑÐ°Ð²Ð° Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ´Ñ Ð´Ð»Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ð¸ Vischeria punctata
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Optimization of the composition of the nutrient medium for the cultivation of micro-algae Vischeria punctata
Creators: name: ÐÐµÑÐµÐ·Ð¸Ð½Ð°, ÐÑÐµÐ½Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: vischeria punctata
ÐºÑÐ¸Ð²ÑÐµ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ
Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½ÑÐµ ÑÑÐµÐ´Ñ
Ð±Ð¸Ð¾Ð¼Ð°ÑÑÐ°
ÑÑÑÐ¿ÐµÐ½Ð·Ð¸Ñ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹
vischeria punktata
cultivation curves
nutrient media
biomass
microalgae suspension

description: ÐÐ° Ð¾ÑÐ½Ð¾Ð²Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¸ Ð²Ð·ÑÑÑ ÑÐµÐºÐ¾Ð¼ÐµÐ½Ð´Ð¾Ð²Ð°Ð½Ð½ÑÐµ ÑÑÐµÐ´Ñ Ð´Ð»Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÑÑÐ°Ð¼Ð¼Ð° IPPAS H-242 Ð¸Ð· ÑÐ¾Ð²ÑÐµÐ¼ÐµÐ½Ð½ÑÑ Ð±Ð°Ð· Ð´Ð°Ð½Ð½ÑÑ Ð² Ð¾Ð±Ð»Ð°ÑÑÐ¸ Ð±Ð¸Ð¾ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¸ Ð°Ð»ÑÐ³Ð¾Ð»Ð¾Ð³Ð¸Ð¸: Â«ÐÐ¾Ð»Ð»ÐµÐºÑÐ¸Ñ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹ Ð¸ ÑÐ¸Ð°Ð½Ð¾Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ IPPAS ÐÐ¤Ð  Ð ÐÐÂ»; Â«Listing the Worldâs Algae AlgaeBaseÂ»; Â«ÐÐ¾Ð»Ð»ÐµÐºÑÐ¸Ñ ÐºÑÐ»ÑÑÑÑ Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹ Ð² ÐÐµÑÑÐ¸Ð½Ð³ÐµÐ½ÑÐºÐ¾Ð¼ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑÐµ (SAG)Â». Ð ÐµÐºÐ¾Ð¼ÐµÐ½Ð´Ð¾Ð²Ð°Ð½Ð½ÑÐµ ÑÑÐµÐ´Ñ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð»Ð¸ÑÑ Ð´Ð¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸ÐµÐ¼ Ð²Ð¸ÑÐ°Ð¼Ð¸Ð½Ð¾Ð²: Ð¿Ð¸ÑÐ¸Ð´Ð¾ÐºÑÐ¸Ð½Ð°, ÑÐ¸Ð°Ð¼Ð¸Ð½Ð°, ÑÐ¸Ð°Ð½Ð¾ÐºÐ¾Ð±Ð¾Ð»Ð°Ð¼Ð¸Ð½Ð°, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ° Â«CO2 PlusÂ». Ð¦ÐµÐ»Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð¾Ð¿ÑÐ¸Ð¼Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÐ¾ÑÑÐ°Ð²Ð° Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ´Ñ Ð´Ð»Ñ ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ñ Ð²ÑÑÐ¾Ð´Ð° Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð¿ÑÐ¸ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ð¸ Vischeria punctata. ÐÐ°Ð´Ð°ÑÐ¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: Ë Ð¿ÑÐ¾Ð²ÐµÑÑÐ¸ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Vischeria punctata Ð² Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼ÑÑ ÑÑÐµÐ´Ð°Ñ Ð¸ Ð¿Ð¾-ÑÑÑÐ¾Ð¸ÑÑ ÐºÑÐ¸Ð²ÑÐµ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ; Ë Ð¿ÑÐ¾Ð²ÐµÑÑÐ¸ ÑÐ°ÑÑÐµÑ ÐºÐ¸Ð½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÐ¾Ð² ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ; Ð¿ÑÐ¾Ð²ÐµÑÑÐ¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¼Ð¾ÑÑÐ¾-ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸Ðº Ð¿Ð¾Ð¿ÑÐ»ÑÑÐ¸Ð¸ Vischeria punctata Ð¿ÑÐ¸ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸; Ë Ð¾Ð¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ Ð²ÑÑÐ¾Ð´ Ð²Ð¾Ð·Ð´ÑÑÐ½Ð¾-ÑÑÑÐ¾Ð¹ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð½Ð° ÐºÐ¾Ð½ÐµÑ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð² ÐºÐ°Ð¶Ð´Ð¾Ð¼ Ð²Ð°ÑÐ¸Ð°Ð½ÑÐµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ. ÐÐ»Ñ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÐ¸ Ð¸ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ Ð¿Ð°ÐºÐµÑ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼ Microsoft Office, Microsoft ExÑel, Ð´Ð»Ñ Ð¼Ð¾ÑÑÐ¾Ð¼ÐµÑÑÐ¸Ð¸ Ë ÐÐ¡-foto Ð¸ Levenhuk. ÐÐ°Ð¿Ð¸ÑÐ°Ð½Ð¸Ðµ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±Ð·Ð¾ÑÐ° Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»Ð¾ÑÑ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ñ Ð±Ð°Ð· Ð´Ð°Ð½Ð½ÑÑ: ÐµLIBRARY.RU, Scopus. Ð Ð°Ð±Ð¾ÑÐ° Ð²ÑÐ¿Ð¾Ð»Ð½ÑÐ»Ð°ÑÑ Ð² Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ ÐÐ¨ÐÐ¢Ð¸ÐÐ Ð¡ÐÐÐÐÐ£ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾. ÐÑÐ»Ð¾ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¾, ÑÑÐ¾ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ ÑÑÑÐµÐºÑÐ¸Ð²Ð½ÑÐ¼Ð¸ Ð¾ÐºÐ°Ð·Ð°Ð»Ð¸ÑÑ ÑÑÐµÐ´Ñ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ ÑÑÐµÐ´Ñ ÐÐ¾Ð»Ð´Ð° (ÐÐÐ 3N) Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐµ Ð´Ð¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸ÐµÐ¼ Ð²Ð¸ÑÐ°Ð¼Ð¸Ð½Ð¾Ð² Ð¸ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ° Ð¡Ð2. ÐÐ°Ð¸Ð±Ð¾Ð»ÑÑÐ¸Ð¹ Ð²ÑÑÐ¾Ð´ Ð²Ð¾Ð·Ð´ÑÑÐ½Ð¾-ÑÑÑÐ¾Ð¹ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ (Ð±Ð¾Ð»ÐµÐµ 2,5 Ð³ Ð½Ð° Ð»Ð¸ÑÑ ÑÑÑÐ¿ÐµÐ½Ð·Ð¸Ð¸) Ð½Ð°Ð±Ð»ÑÐ´Ð°ÐµÑÑÑ Ð¿ÑÐ¸ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ Vischeria punctata Ð² ÑÑÐµÐ´Ðµ Ñ Ð´Ð¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸Ðµ Ð¿Ð¸ÑÐ¸Ð´Ð¾ÐºÑÐ¸Ð½Ð°. ÐÑÐ¼ÐµÑÐ°ÐµÑÑÑ Ð¾ÑÑÑÑÑÑÐ²Ð¸Ðµ Ð²ÑÑÐ°Ð¶ÐµÐ½Ð½Ð¾Ð¹ Ð»Ð°Ð³-ÑÐ°Ð·Ñ, ÑÐ°Ð·Ð° ÑÑÐ°Ð±Ð¸Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð½Ð°ÑÑÑÐ¿Ð°ÐµÑ Ð´Ð»Ñ Ð±Ð¾Ð»ÑÑÐ¸Ð½ÑÑÐ²Ð° Ð²Ð°ÑÐ¸Ð°Ð½ÑÐ¾Ð² Ð½Ð° Ð²Ð¾ÑÑÐ¼ÑÐµ ÑÑÑÐºÐ¸, Ð² ÑÑÐµÐ´Ðµ Ñ Ð¿Ð¸ÑÐ¸Ð´Ð¾ÐºÑÐ¸Ð½Ð¾Ð¼ Ð² ÑÑÐ¾ Ð²ÑÐµÐ¼Ñ ÑÐ¾ÑÑÐ°Ð½ÑÐµÑÑÑ ÑÐ¾ÑÑ Ð¿Ð¾Ð¿ÑÐ»ÑÑÐ¸Ð¸. Ð Ð¿Ð¾Ð¿ÑÐ»ÑÑÐ¸Ð¸ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ð¸, Ð² ÑÑÐµÐ´Ðµ Ñ Ð¿Ð¸ÑÐ¸Ð´Ð¾ÐºÑÐ¸Ð½Ð¾Ð¼ Ð² ÑÑÐµÐ´Ð½ÐµÐ¼, ÐºÐ»ÐµÑÐºÐ¸ Ð±Ð¾Ð»ÐµÐµ ÐºÑÑÐ¿Ð½ÑÐµ (Ð¿Ð»Ð¾ÑÐ°Ð´Ñ Ð¿ÑÐ¾ÐµÐºÑÐ¸Ð¸ Ð±Ð¾-Ð»ÐµÐµ 17,08 Ð¼ÐºÐ¼2), Ð´Ð¾Ð»Ñ ÐºÐ»ÐµÑÐ¾Ðº Ñ Ð°Ð²ÑÐ¾ÑÐ¿Ð¾ÑÐ°Ð¼Ð¸ Ð²Ð¾Ð·ÑÐ°ÑÑÐ°ÐµÑ. ÐÐ»Ñ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ¸ ÑÐ¾ÑÑÐ° Ð¿Ð¾Ð¿ÑÐ»ÑÑÐ¸Ð¸ Vischeria punctata Ð² Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼ÑÑ ÑÑÐµÐ´Ð°Ñ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÑÐ»Ð¸ ÐºÐ¸Ð½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ðµ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÑ ÑÐ¾ÑÑÐ°: ÐµÐ´Ð¸Ð½Ð¸ÑÐ½ÑÑ ÑÐºÐ¾ÑÐ¾ÑÑÑ ÑÐ¾ÑÑÐ° Ð² ÑÐ°Ð·Ñ ÑÐºÑÐ¿Ð¾Ð½ÐµÐ½ÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÐ¾ÑÑÐ° (Î¼), Ð¿Ð¾Ð¿ÑÐ»ÑÑÐ¸Ð¾Ð½Ð½ÑÑ ÐµÐ¼ÐºÐ¾ÑÑÑ; Ð¿Ð¾ÑÑÑÐ¾ÐµÐ½Ñ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ, Ð¾Ð¿Ð¸ÑÑÐ²Ð°ÑÑÐ¸Ðµ ÑÐ¾ÑÑ Ð¿Ð¾Ð¿ÑÐ»ÑÑÐ¸Ð¸ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ð¸ Ð² ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ ÑÑÐµÐ´Ð°Ñ Ð½Ð° Ð¾Ñ-Ð½Ð¾Ð²Ðµ Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð¤ÐµÑÑÑÐ»ÑÑÑÐ°. ÐÐ»Ñ Ð¾ÑÐµÐ½ÐºÐ¸ ÑÐ¾ÑÑÐ¾ÑÐ½Ð¸Ñ Ð¿Ð¾Ð¿ÑÐ»ÑÑÐ¸Ð¸ Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»Ð¸ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸ÑÐµÑÐºÐ¾Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¿ÑÐ¸Ð¶Ð¸Ð·Ð½ÐµÐ½Ð½ÑÑ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð² Â«ÑÐ°Ð·Ð´Ð°Ð²Ð»ÐµÐ½Ð½Ð°Ñ ÐºÐ°Ð¿Ð»ÑÂ» Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð° ÐÐ°Ð±Ð¾ÐÐµÐ´-2. ÐÐ¸ÐºÑÐ¾ÑÐ¾ÑÐ¾Ð³ÑÐ°ÑÐ¸Ð¸ Ð´ÐµÐ»Ð°Ð»Ð¸ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÑÐ¸ÑÑÐ¾Ð²Ð¾Ð¹ ÐºÐ°Ð¼ÐµÑÑ IS-500. 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description: The study was based on the recommended media for cultivating the strain IP-PAS H-242 from modern databases in the field of biotechnology and algology: Â«Collection of microalgae and cyanobacteria IPPAS IGF RASÂ»; Â«Listing the Worlds Algae AlgaeBaseÂ»; Â«Collection of algae cultures at the University of GÃ¶ttingen (SAG)Â». The recommended media were modified with the addition of vitamins: pyridoxine, thiamine, cyanocobolamine, as well as the preparation Â«CO2 PlusÂ». The aim of the study is to determine the optimal composition of the nutrient medium to increase the biomass yield during the cultivation of the microalgae Vischeria punctata. Research objectives: Ë to carry out the cultivation of Vischeria punctata in the studied media and to construct cultivation curves; Ë to calculate the kinetic parameters of cultivation; to study the morpho-physiological characteristics of the Vischeria punctata population during cultivation; Ë determine the yield of air-dry biomass at the end of cultivation in each variant of the study. To process and analyze the data obtained, Microsoft Office, Microsoft Excel, MS-foto and Levenhuk software packages were used. The literature review was writ-ten using the following databases: Library.RU, Scopus. The work was carried out in the laboratory of the SSBTiPP of Peter the Great St. Petersburg State Pedagogical University. It was determined that the most effective media were based on the Bold medium (BBM 3N) modified with the addition of vitamins and a CO2 preparation. The highest yield of air-dry biomass (more than 2.5 g per liter of suspension) is observed when cultivating Vischeria punctata in an environment with the addition of pyridoxine. There is no pronounced lag phase, the stabilization phase occurs for most variants on the eighth day, in an environment with pyridoxine at this time, population growth persists. In the microalgae population, in the medium with pyridoxine, on average, the cells are larger (the projection area is more than 17.08 microns 2), the proportion of cells with autospores increases. To characterize the growth of the Vischeria punctata population in the studied media, kinetic growth parameters were determined: the unit growth rate in the exponential growth phase (Âµ), population capacity; equations describing the growth of the microalgae population in various media were constructed based on the Ferhulst model. To assess the state of the population, microscopic examination of lifetime preparations Â«crushed dropÂ» was performed using a LaboMed-2 microscope. Micrographs were taken using an IS-500 digital camera. The photos were taken using the MS-foto computer program, the photos were processed using the Levenhuk program. Microscopic examination of the population was carried out in the stabilization phase, morphometry was carried out when measuring 50 cells in at least five fields of view. The signs characterizing the cells during the formation of autospores, flocculating cells, and cells susceptible to apoptosis were taken into account. The Levenhuk program tools determined the size of cells, the shape of the cell was determined as an ellipsoid of rotation. The elongation coefficient, cell volume and surface area were calculated. Morphometric characteristics made it possible to analyze the morpho-physiological state of cells in the population at the end of culturing and obtaining biomass.