title: Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½ÑÑ ÑÑÐµÐ´ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¼ÐµÐ»Ð°ÑÑÑ Ð´Ð»Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Chlamydomonas geitleri Ettl
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Development of nutrient media using molasses for the cultivation of Chlamydomonas geitleri Ettl
Creators: name: ÐÐ°ÐºÐ¸ÑÐ¾Ð²Ð°, ÐÐ°ÑÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Chlamydomonas geitleri Ettl
ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½ÑÐµ ÑÑÐµÐ´Ñ
Ð¼ÐµÐ»Ð°ÑÑÐ°
Ð¼Ð¾ÑÑÐ¾Ð¼ÐµÑÑÐ¸Ñ
Ð±Ð¸Ð¾Ð¼Ð°ÑÑÐ°
cultivation
nutrient media
molasses
morphometry
biomass

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð½Ð°Ð¸Ð»ÑÑÑÐ¸Ñ ÑÑÐ»Ð¾Ð²Ð¸Ð¹ Ð´Ð»Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¼ÐµÐ»Ð°ÑÑÑ Ð² ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ Chlamydomonas geitleri Ettl (ÑÑÐ°Ð¼Ð¼ IPPASD-231). ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: - Ð¿ÑÐ¾Ð²ÐµÑÑÐ¸ Ð°ÐºÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð¼Ð°ÑÐ¾ÑÐ½Ð¾Ð¹ ÐºÑÐ»ÑÑÑÑÑ Ch. geitleri; - Ð¾Ð¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ Ð½Ð°Ð¸Ð»ÑÑÑÑÑ Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½ÑÑ ÑÑÐµÐ´Ñ Ð´Ð»Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ch. geitleri Ettl Ð² ÑÐµÐºÐ¾Ð¼ÐµÐ½Ð´ÑÐµÐ¼ÑÑ Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½ÑÑ ÑÑÐµÐ´Ð°Ñ Ð¿Ð¾ ÐºÐ¸Ð½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ð¼ Ð¿Ð¾ÐºÐ°Ð·Ð°ÑÐµÐ»ÑÐ¼ ÑÐ¾ÑÑÐ° Ð¸ Ð¼Ð¾ÑÑÐ¾Ð¼ÐµÑÑÐ¸ÑÐµÑÐºÐ¸Ð¼ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÐ°Ð¼ Ð¿Ð¾Ð¿ÑÐ»ÑÑÐ¸Ð¸, Ð¾Ð¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ Ð²ÑÑÐ¾Ð´ Ð²Ð¾Ð·Ð´ÑÑÐ½Ð¾-ÑÑÑÐ¾Ð¹ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ; - Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÑ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÑ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¡h. geitleri Ð² ÑÐ°Ð·Ð½ÑÑ ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÐ½ÑÑ Ð´Ð¸Ð°Ð¿Ð°Ð·Ð¾Ð½Ð°Ñ; - Ð¿ÑÐ¾Ð²ÐµÑÑÐ¸ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ð¸ Ð² ÑÑÐµÐ´Ð°Ñ Ñ Ð´Ð¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸ÐµÐ¼ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ Ð¸ÑÑÐ¾ÑÐ½Ð¸ÐºÐ¾Ð² ÑÐ³Ð»ÐµÑÐ¾Ð´Ð° (Ð°ÑÐµÑÐ°ÑÐ° Ð½Ð°ÑÑÐ¸Ñ, Ð³Ð»ÑÐºÐ¾Ð·Ñ Ð¸ Ð¼ÐµÐ»Ð°ÑÑÑ), Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÑ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ ÑÐ°Ð·Ð½ÑÑ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¹ Ð¼ÐµÐ»Ð°ÑÑÑ Ð² Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ´Ðµ Ð½Ð° Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÑ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¡h. geitleri, Ð¾Ð¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ Ð²ÑÑÐ¾Ð´ Ð²Ð¾Ð·Ð´ÑÑÐ½Ð¾-ÑÑÑÐ¾Ð¹ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ. Ð Ð°Ð±Ð¾ÑÐ° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ ÐÐ¨ÐÐ¸ÐÐ Ð¡ÐÐ±ÐÐ£. Ð Ð´Ð°Ð½Ð½Ð¾Ð¼ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¸ Ð±ÑÐ»Ð¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ: Ðlibrary, Â«ÐÐ¾Ð»Ð»ÐµÐºÑÐ¸Ñ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹ Ð¸ ÑÐ¸Ð°Ð½Ð¾Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ IPPAS ÐÐ¤Ð  Ð ÐÐÂ»; Â«Listing the Worldâs Algae AlgaeBaseÂ»; Â«ÐÐ¾Ð»Ð»ÐµÐºÑÐ¸Ñ ÐºÑÐ»ÑÑÑÑ Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹ Ð² ÐÐµÑÑÐ¸Ð½Ð³ÐµÐ½ÑÐºÐ¾Ð¼ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑÐµ (SAG)Â». ÐÐ»Ñ Ð¿ÑÐ¾Ð¸Ð·Ð²ÐµÐ´ÐµÐ½Ð¸Ñ ÑÐ°ÑÑÐµÑÐ¾Ð² Ð¸ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»ÑÑ Ð¿Ð°ÐºÐµÑ Ð´Ð¾ÐºÑÐ¼ÐµÐ½ÑÐ¾Ð² Microsoft Excel. ÐÐ»Ñ Ð¼Ð¾ÑÑÐ¾Ð¼ÐµÑÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸ÑÐµÑÐºÐ¾Ð¹ ÐºÐ°ÑÑÐ¸Ð½Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ñ ÐÐ¡-foto Ð¸ Levenhuk. Ð ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ñ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ: Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð°Ñ Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½Ð°Ñ ÑÑÐµÐ´Ð° Ð´Ð»Ñ Ð°ÐºÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¼Ð°ÑÐ¾ÑÐ½Ð¾Ð¹ ÐºÑÐ»ÑÑÑÑÑ Ð¸ ÐµÐµ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐµÐ³Ð¾ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÑÐ²Ð»ÑÐµÑÑÑ ÑÑÐµÐ´Ð°, Ð¾Ð¿Ð¸ÑÐ°Ð½Ð½Ð°Ñ Ð² Ð¿Ð°ÑÐµÐ½ÑÐµ â 113035. ÐÑÑÐ»ÐµÐ´ÑÐµÐ¼ÑÐ¹ ÑÑÐ°Ð¼Ð¼ ÑÐ»Ð°Ð¼Ð¸Ð´Ð¾Ð¼Ð¾Ð½Ð°Ð´Ñ ÑÐ¿Ð¾ÑÐ¾Ð±ÐµÐ½ ÑÐ°ÑÑÐ¸ Ð² ÑÐ¸ÑÐ¾ÐºÐ¾Ð¼ Ð´Ð¸Ð°Ð¿Ð°Ð·Ð¾Ð½Ðµ ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑ: Ð¾Ñ 8 Ð´Ð¾ 26 Â°Ð¡. ÐÐ¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸Ðµ Ð² ÑÑÐµÐ´Ñ Ð°ÑÐµÑÐ°ÑÐ° Ð½Ð°ÑÑÐ¸Ñ (2 Ð³/Ð») Ð¸ Ð³Ð»ÑÐºÐ¾Ð·Ñ (1 Ð³/Ð») Ð¿ÑÐ¸Ð²Ð¾Ð´Ð¸Ñ Ðº ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ñ Ð²ÑÑÐ¾Ð´Ð° Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ð¸, ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾, Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼ÑÐ¹ ÑÑÐ°Ð¼Ð¼ ÑÐ¿Ð¾ÑÐ¾Ð±ÐµÐ½ Ðº Ð¼Ð¸ÐºÑÐ¾ÑÑÐ¾ÑÐ½Ð¾Ð¼Ñ Ð¿Ð¸ÑÐ°Ð½Ð¸Ñ. ÐÐ½Ð°Ð»Ð¸Ð· ÐºÐ¸Ð½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÐ¾Ð² ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¡h. geitleri Ð½Ð° ÑÑÐµÐ´Ð°Ñ Ñ Ð´Ð¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸ÐµÐ¼ Ð¼ÐµÐ»Ð°ÑÑÑ, Ð¿Ð¾ÐºÐ°Ð·Ð°Ð», ÑÑÐ¾ Ð¼Ð°ÐºÑÐ¸Ð¼Ð°Ð»ÑÐ½Ð¾Ð¹ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸ÐµÐ¹ Ð¼ÐµÐ»Ð°ÑÑÑ, ÐºÐ¾ÑÐ¾ÑÑÑ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð° ÑÑÐ²Ð°Ð¸Ð²Ð°ÑÑ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ñ ÑÐ²Ð»ÑÐµÑÑÑ 10 Ð³/Ð», Ð¿ÑÐ¸ ÑÑÐ¾Ð¼ ÑÐ²ÐµÐ»Ð¸ÑÐ¸Ð²Ð°ÐµÑÑÑ Ð²ÑÑÐ¾Ð´ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ð¸, Ð° ÑÑÐµÐ´Ð½Ð¸Ð¹ ÑÐ°Ð·Ð¼ÐµÑ ÐºÐ»ÐµÑÐ¾Ðº Ð² Ð¿Ð¾Ð¿ÑÐ»ÑÑÐ¸Ð¸ ÑÐ¼ÐµÐ½ÑÑÐ°ÐµÑÑÑ.
description: This work is devoted to determining the best conditions for the use of molasses in the cultivation of Chlamydomonas geitleri Ettl (strain IPPASD-231). Tasks that were solved during the research: - to activate the uterine culture of Ch. geitleri; - to determine the best nutrient medium for cultivating Ch. geitleri Ettl in recommended nutrient media based on kinetic growth indicators and morphometric parameters of the population, to determine the yield of air-dry biomass; - to investigate the parameters of cultivation of C. geitleri in different temperature ranges; - to cultivate microalgae in media with the addition of various carbon sources (sodium acetate, glucose and molasses), to investigate the effect of different concentrations of molasses in the nutrient medium on the parameters of cultivation of Ch. geitleri, to determine the yield of air-dry biomass. The work was carried out on the basis of the Graduate School of Biotechnology and Food Production of IBSiB. The following databases were used in this study: Library, "Collection of microalgae and cyanobacteria IPPAS IPP RAS"; "Listing the Worlds Algae AlgaeBase"; "Collection of algae cultures at the University of GÃ¶ttingen (SAG)". A Microsoft Excel document package was used to perform calculations and analysis. The MS-foto and Levenhuk programs were used for morphometric examination of the microscopic picture. During the study, the following results were obtained: the most effective nutrient medium for activating the uterine culture and its subsequent cultivation is the medium described in patent Nb. 113035. The studied strain of Chlamydomonas is able to grow in a wide temperature range: from 8 to 26 Â° C. The addition of sodium acetate (2 g/l) and glucose (1 g/l) to the medium leads to an increase in the yield of microalgae biomass, therefore, the studied strain is capable of mixotrophic nutrition. Analysis of kinetic parameters of cultivation of Ch. geitleri on molasses-added media showed that the maximum concentration of molasses that microalgae can absorb is 10 g/l, while increasing the yield of microalgae biomass, and the average cell size in the population decreases.