Additional file 1 of Fetal Muse-based therapy prevents lethal radio-induced gastrointestinal syndrome by intestinal regeneration
Creators: name: Dushime, Honorine
name: Moreno, Stéphanie G.
name: Linard, Christine
name: Adrait, Annie
name: Couté, Yohann
name: Peltzer, Juliette
name: Messiaen, Sébastien
name: Torres, Claire
name: Bensemmane, Lydia
name: Lewandowski, Daniel
name: Romeo, Paul-Henri
name: Petit, Vanessa
name: Gault, Nathalie
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Physiology
subject: Chemical Sciences not elsewhere classified
subject: Immunology
subject: FOS: Clinical medicine
subject: Developmental Biology
subject: Cancer
subject: Hematology
subject: Computational Biology

description: Additional file: Fig. S1. A Representative flow cytometry plots showing the viability (Hoechst) and phenotype (CD45, CD44, CD105, CD90 and CD73) of WJ-MSC before culture amplification. B Representative flow cytometry plots showing the percentage of SSEA-3+ Muse cells obtained after amplification of BM-MSC or WJ-MSC at passage 7. C Illustration of BM-Muse and WJ-Muse after culture in adherence condition (top) and BM-Muse and WJ-Muse clusters spontaneously obtained after culture in methylcellulose (bottom). D Volcano plot displaying the differential abundance of proteins in BM-Muse and WJ-Muse cells analyzed by MS-based label-free quantitative proteomics. Green and blue dots represent proteins found significantly enriched, respectively, in BM-Muse and WJ-Muse cells (fold change ≥ 2 and p value ≤ 0.005, leading to a Benjamini–Hochberg FDR &lt; 1%). E Comparative analysis of common biological processes occurring in BM-Muse and WJ-Muse cells, identified by proteomic analysis. Fig. S2 (relative to Fig. 3). A Representative flow cytometry plots showing the viability (Hoechst) of thawed WJ-Muse cells and their SSEA-3 marker expression maintenance. B Kaplan–Meier survival analysis (upper panels) and weight loss changes (lower panels) for 30 days of 18 Gy abdominal exposed mice receiving either 50,000 freshly isolated Muse cells (left panels) or 50,000 cryo-preserved Muse cells (right panels) 4 h after irradiation. Statistical difference in survival between groups was determined by Log-rank (Mantel-Cox) test; *p ≤ 0.05; **p ≤ 0.01. Weight data are represented with means ± SEM. Fig. S3 (relative to Fig. 6). A Representative flow cytometry gating strategy for analyze and isolation of lamina epithelialis subpopulations enriched in stem cells (green box) or Paneth cells (red box). B Quantitative RT-qPCR analysis showing the expression markers of stem cells (Lgr5, Olfm4, Ascl2) and Paneth cells (Lysozyme) in isolated subpopulations, compared to CD24/CD166 double negative intestinal epithelial cells (black box). Fig. S4 (relative to Fig. 6). A Representative full-length WES showing the protein level of cleaved caspase-3 in Paneth cells isolated from non-treated and Muse-treated mice at 1 day after irradiation. GAPDH was used as an internal control. The framed areas correspond to the illustration of the cropping gel in the Fig. 6D. B Representative full-length WES showing the protein levels of p-Stat3 and Stat3 in Paneth cells isolated from non-treated and Muse-treated mice at 1 day after irradiation. GAPDH was used as an internal control. LPS-treated RAW cells were used as p-Stat3 positive control (CTL). The framed areas correspond to the illustration of the cropping gel in the Fig. 6F.