Cloning and expression analysis of PRL and PRLR genes in Black Muscovy Duck
Creators: name: X. Li
name: W. Ji
name: G. Sun
name: W. Xiao
name: Y. Bian
name: H. Qing
Publisher: name: Taylor & Francis
Year: 2019
Subjects: subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Physiology
subject: Biotechnology
subject: Evolutionary Biology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: FOS: Chemical sciences
subject: Ecology
subject: Immunology
subject: FOS: Clinical medicine
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Inorganic Chemistry
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: FOS: Health sciences

description: Prolactin hormone (governed by the <i>PRL</i> gene) is secreted by the anterior pituitary of animals, which combines with its receptor (prolactin receptor, <i>PRLR</i>) to act on target cells. Both <i>PRL</i> and <i>PRLR</i> are mainly associated with reproductive performance. The genetic mechanism of nesting in poultry is not yet clear, and so the aim of the current study was to determine expression patterns of <i>PRL</i> and <i>PRLR</i> at different times across the breeding stages of black Muscovy ducks.In this study, the CDS regions of <i>PRL</i> and <i>PRLR</i> were determined by RACE sequencing. The expression levels of <i>PRL</i> and <i>PRLR</i> in the pituitary, ovary and uterus from the black Muscovy duck were compared and analysed during the pre-laying, laying and nesting periods.The results showed that <i>PRL</i> and <i>PRLR</i> are highly homologous in a variety of poultry sequences. The expression of the <i>PRL</i> gene in the pituitary was the highest, which was significantly higher than seen in the ovary and uterus. This trend ran through the entire prenatal period, i.e. the laying period and the nesting period. The expression level of the <i>PRLR</i> gene in the pituitary and ovary was generally low, and expression in the uterus was the highest. There was no significant difference in expression of the <i>PRLR</i> gene between pituitary and ovary during different periods, but the expression level of the <i>PRLR</i> gene in the uterus reached its highest level during the nesting stage, which was significantly higher than seen in the early laying period. Prolactin hormone (governed by the <i>PRL</i> gene) is secreted by the anterior pituitary of animals, which combines with its receptor (prolactin receptor, <i>PRLR</i>) to act on target cells. Both <i>PRL</i> and <i>PRLR</i> are mainly associated with reproductive performance. The genetic mechanism of nesting in poultry is not yet clear, and so the aim of the current study was to determine expression patterns of <i>PRL</i> and <i>PRLR</i> at different times across the breeding stages of black Muscovy ducks. In this study, the CDS regions of <i>PRL</i> and <i>PRLR</i> were determined by RACE sequencing. The expression levels of <i>PRL</i> and <i>PRLR</i> in the pituitary, ovary and uterus from the black Muscovy duck were compared and analysed during the pre-laying, laying and nesting periods. The results showed that <i>PRL</i> and <i>PRLR</i> are highly homologous in a variety of poultry sequences. The expression of the <i>PRL</i> gene in the pituitary was the highest, which was significantly higher than seen in the ovary and uterus. This trend ran through the entire prenatal period, i.e. the laying period and the nesting period. The expression level of the <i>PRLR</i> gene in the pituitary and ovary was generally low, and expression in the uterus was the highest. There was no significant difference in expression of the <i>PRLR</i> gene between pituitary and ovary during different periods, but the expression level of the <i>PRLR</i> gene in the uterus reached its highest level during the nesting stage, which was significantly higher than seen in the early laying period.