title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ ÑÐ¾ÑÐµÑÐ½ÑÑ Ð¼ÑÑÐ°ÑÐ¸Ð¹ Ð² Ð¼Ð¾Ð»ÐµÐºÑÐ»Ðµ ÑÐ¸Ð³Ð¼Ð°-1 ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° Ð½Ð° ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¸Ñ ÐºÐ¾Ð½ÑÐ°ÐºÑÐ¾Ð² Ð½ÐµÐ¹ÑÐ¾Ð½Ð¾Ð² ÑÑÑÐ¸Ð°ÑÑÐ¼Ð° Ð½Ð° Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ Ð¥Ð°Ð½ÑÐ¸Ð½Ð³ÑÐ¾Ð½Ð°
title: Ð¼Ð°Ð³Ð¸ÑÑÐµÑÑÐºÐ°Ñ Ð´Ð¸ÑÑÐµÑÑÐ°ÑÐ¸Ñ
titleType: Subtitle
Creators: name: ÐÑÐºÐ°Ð½Ð¾Ð²Ð°, ÐÐºÐ°ÑÐµÑÐ¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2016
Subjects: ÐÐµÐ»ÐºÐ¸
ÐÐµÐ¹ÑÐ¾Ð½Ñ
Ð±Ð¾Ð»ÐµÐ·Ð½Ñ Ð¥Ð°Ð½ÑÐ¸Ð½Ð³ÑÐ¾Ð½Ð°
ÑÐ¸Ð³Ð¼Ð°-1 ÑÐµÑÐµÐ¿ÑÐ¾Ñ
ÐºÐ¾ÑÑÐ¸ÐºÐ¾-ÑÑÑÐ¸Ð°ÑÐ½Ð°Ñ ÐºÑÐ»ÑÑÑÑÐ°
Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÐµ ÑÐ¸Ð¿Ð¸ÐºÐ¸
Huntington's disease
Sigma-1 receptor
cortico-striata culture
dendritic spines

description: Ð ÑÐ°Ð±Ð¾ÑÐµ Ð±ÑÐ»Ð° Ð¸Ð·ÑÑÐµÐ½Ð° ÑÐ¾Ð»Ñ ÑÐ¸Ð³Ð¼Ð°-1 ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° Ð² ÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¸Ñ ÐºÐ¾Ð½ÑÐ°ÐºÑÐ¾Ð² Ð½ÐµÐ¹ÑÐ¾Ð½Ð¾Ð² ÑÑÑÐ¸Ð°ÑÑÐ¼Ð° Ð½Ð° Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ Ð¥Ð°Ð½ÑÐ¸Ð½Ð³ÑÐ¾Ð½Ð° in vitro. ÐÑÐ»Ð¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð¼ÐµÑÐ¾Ð´Ñ: Ð³ÐµÐ½Ð¾ÑÐ¸Ð¿Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð¼ÑÑÐµÐ¹, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ðµ Ð¸ Ð¿Ð¾Ð´Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ðµ ÐºÐ¾ÑÑÐ¸ÐºÐ¾-ÑÑÑÐ¸Ð°ÑÐ½Ð¾Ð¹ Ð½ÐµÐ¹ÑÐ¾Ð½Ð°Ð»ÑÐ½Ð¾Ð¹ ÐºÑÐ»ÑÑÑÑÑ, Ð¸Ð¼Ð¼ÑÐ½Ð¾ÑÐ¸ÑÐ¾ÑÐ¸Ð¼Ð¸ÑÐµÑÐºÐ¾Ðµ Ð¾ÐºÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ðµ, ÐºÐ¾Ð½ÑÐ¾ÐºÐ°Ð»ÑÐ½Ð°Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ, Ð²Ð¸ÑÑÑÐ½Ð°Ñ ÑÑÐ°Ð½ÑÐ´ÑÐºÑÐ¸Ñ, ÐÐµÑÑÐµÑÐ½ Ð±Ð»Ð¾Ñ Ð°Ð½Ð°Ð»Ð¸Ð·, ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²ÐµÐ½Ð½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð· Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÑ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð² Ð² Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ðµ NeuronStudio Ð¸ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð±ÐµÐ»ÐºÐ¾Ð² Ð² Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ðµ QuantityOne. Ð ÑÐ°Ð±Ð¾ÑÐµ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾ Ð½Ð°ÑÑÑÐµÐ½Ð¸Ðµ ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¸Ñ ÐºÐ¾Ð½ÑÐ°ÐºÑÐ¾Ð² Ð¼ÐµÐ¶Ð´Ñ Ð½ÐµÐ¹ÑÐ¾Ð½Ð°Ð¼Ð¸ ÑÑÑÐ¸Ð°ÑÑÐ¼Ð° Ð¸ Ð½ÐµÐ¹ÑÐ¾Ð½Ð°Ð¼Ð¸ ÐºÐ¾ÑÑ Ñ Ð¼ÑÑÐµÐ¹ Ñ Ð¼ÑÑÐ°Ð½ÑÐ½ÑÐ¼ Ð³ÐµÐ½Ð¾Ð¼ ÑÐ°Ð½ÑÐ¸Ð½Ð³ÑÐ¸Ð½Ð° Ð½Ð° 21 Ð´ÐµÐ½Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ in vitro Ð¿Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ Ñ 14 Ð´Ð½ÐµÐ¼. ÐÑÑÐ²Ð»ÐµÐ½Ð¾, ÑÑÐ¾ Ð³Ð¸Ð¿ÐµÑÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ ÑÐ¸Ð³Ð¼Ð°-1 ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° Ñ ÑÐ°Ð¹Ñ-Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð½ÑÐ¼Ð¸ Ð¼ÑÑÐ°ÑÐ¸ÑÐ¼Ð¸ Ð¿Ð¾ 119 Ð¸ 120 Ð°Ð¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾ÑÐ°Ð¼ Ð½Ðµ Ð²Ð¾ÑÑÑÐ°Ð½Ð°Ð²Ð»Ð¸Ð²Ð°ÐµÑ ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¸Ñ ÐºÐ¾Ð½ÑÐ°ÐºÑÐ¾Ð² Ð² Ð½ÐµÐ¹ÑÐ¾Ð½Ð°Ñ Ð¼ÑÑÐµÐ¹ Ð»Ð¸Ð½Ð¸Ð¸ YAC128, Ð² Ð¾ÑÐ»Ð¸ÑÐ¸Ðµ Ð¾Ñ Ð³Ð¸Ð¿ÐµÑÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð¿Ð¾ 121 Ð¸ 126 Ð°Ð¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾ÑÐ°Ð¼. ÐÐ¾Ð¶Ð½Ð¾ Ð¿ÑÐµÐ´Ð¿Ð¾Ð»Ð¾Ð¶Ð¸ÑÑ, ÑÑÐ¾ 119 Ð¸ 120 Ð°Ð¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾ÑÑ Ð½Ðµ Ð¸Ð³ÑÐ°ÑÑ ÐºÐ»ÑÑÐµÐ²Ð¾Ð¹ ÑÐ¾Ð»Ð¸ Ð² Ð°ÐºÑÐ¸Ð²Ð½Ð¾Ð¼ ÑÐµÐ½ÑÑÐµ ÑÐ¸Ð³Ð¼Ð°-1 ÑÐµÑÐµÐ¿ÑÐ¾ÑÐµ, Ð° 121 Ð¸ 126 Ð°Ð¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾ÑÑ Ð¸Ð¼ÐµÑÑ Ð²Ð°Ð¶Ð½Ð¾Ðµ Ð·Ð½Ð°ÑÐµÐ½Ð¸Ðµ Ð´Ð»Ñ ÑÐ²ÑÐ·ÑÐ²Ð°Ð½Ð¸Ñ ÑÐ¸Ð³Ð¼Ð°-1 ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° Ñ Ð»Ð¸Ð³Ð°Ð½Ð´Ð°Ð¼Ð¸ Ð¸ Ð±ÐµÐ»ÐºÐ¾Ð¼-Ð¿Ð°ÑÑÐ½ÐµÑÐ¾Ð¼. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð¿Ð¾Ð´ÑÐ²ÐµÑÐ¶Ð´Ð°ÑÑ Ð¸Ð´ÐµÑ Ð¾ ÑÐ¾Ð¼, ÑÑÐ¾ ÑÐ¸Ð³Ð¼Ð°-1 ÑÐµÑÐµÐ¿ÑÐ¾Ñ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÑÐµÑ ÑÐ¾Ð±Ð¾Ð¹ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»ÑÐ½ÑÑ Ð¼Ð¸ÑÐµÐ½Ñ Ð´Ð»Ñ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ¸ ÑÐ°ÑÐ¼Ð°ÐºÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð² Ð´Ð»Ñ Ð»ÐµÑÐµÐ½Ð¸Ñ Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ Ð¥Ð°Ð½ÑÐ¸Ð½Ð³ÑÐ¾Ð½Ð°.
description: The role of sigma-1 receptor in the formation of synaptic contacts of the striatum neurons on Huntington's disease model in vitro was studied in the present work. The following methods were used: genotyping of mice, isolating and maintaining the cortico-striatal neuronal culture, immunocytochemical staining, confocal microscopy, viral transduction, Western blot analysis, quantitative analysis of dendritic spines in NeuronStudio program and protein expression in QuantityOne program. It was shown a violation of synaptic contacts between striatum neurons and cortex neurons in mice with mutant huntingtin gene on 21 days in vitro culture compared to 14 day. It was revealed that the overexpression of the sigma-1 receptor with site-directed mutations in 119 and 120 amino acids does not restore neuronal synaptic contacts in mice YAC128 as opposed to overexpression with mutations in 121 and 126 amino acids. It is possible to assume that amino acids 119 and 120 do not play a key role in the active center of the sigma-1 receptor and amino acids 121 and 126 are important for the sigma-1 receptor's binding with ligands and the protein-partner. The obtained results confirm the idea that sigma-1 receptor is a potential pharmacological target for development of drugs for the treatment of Huntington's disease.