Hollow Fiber-in-Syringe Equilibrium Sampling Through Supported-Liquid Membrane for Evaluation of Drug-Plasma Binding
Creators: name: Barri, Thaer
name: Ramzi, Ruba
name: Idkaidek, Nasir M
name: Al-Hashimi, Nabil N
name: Al-Akayleh, Faisal
name: Agha, Ahmed S A Ali
Publisher: name: Taylor & Francis
Year: 2024
Subjects: Biophysics
Biochemistry
Medicine
Pharmacology
Biotechnology
Chemical Sciences not elsewhere classified

description: <b>Aim:</b> The aim was to evaluate drug-plasma binding (DPB).by employing Hollow Fiber-in-Syringe Equilibrium Sampling Through Supported Liquid Membrane (HFiS ESTSLM) and RP-HPLC analysis. <b>Materials &amp; Methods:</b> HFiS ESTSLM and RP-HPLC were used to evaluate DPB of three weak basic drugs (Metoprolol, Diphenhydramine, and Sildenafil) with differing hydrophilicity and binding ability to blood plasma. <b>Results:</b> The results exhibited an increasing drug-dependent magnitude of DPB for the three model drugs. This trend of DPB confirmed that HFiS ESTSLM has the required sensitivity for determining DPB of the drugs. The DPB was drug concentration-dependent within the tested drug concentration range, especially at high concentration. <b>Conclusion:</b> HFiS ESTSLM and RP-HPLC offered a simple, easy and cost-effective procedure to evaluate DPB of these basic drugs. The study introduces a new design of Hollow Fiber-in-Syringe Equilibrium Sampling Through Supported Liquid Membrane (HFiS ESTSLM) to evaluate drug-plasma binding (DPB) for weak basic drugs. This technique addresses the need for precise, cost-effective and efficient methods to measure free drug fractions without disrupting the equilibrium between bound and free drug states. The new setup of HFiS ESTSLM and RP-HPLC are described in detail as the main methodologies employed to assess DPB. The selection of materials and instruments underlines the effort to optimize experimental conditions conducive to accurate DPB assessment. The study confirms that DPB is both drug concentration-dependent and drug-specific. At lower concentrations, the binding patterns remained relatively constant for drugs with higher binding capabilities, whereas, at higher concentrations, a saturation effect was observed due to protein binding capacities being exceeded. In general, the %DPB values of the model drugs were comparable to their literature values. The discussion highlights how the HFiS ESTSLM technique provides a non-depletive method that avoids disturbing the drug-protein equilibrium. The advantages over traditional methods include reduced sample and solvent use, minimal preparation time, and the ability to maintain equilibrium conditions during sampling. This new setup of HFiS ESTSLM combined with RP-HPLC is shown to be an effective and accessible method for determining the extent of DPB across a range of weak basic drugs with varying hydrophilicities and binding characteristics. This method could significantly help pharmacokinetic studies by providing a more nuanced understanding of drug behavior in blood plasma. The new HFiS ESTSLM setup with RP-HPLC analysis offered a simple, easy, relatively fast, and cost-effective method to assess DPB of weak basic drugs that may have different hydrophilicity and binding abilities to blood plasma. The method does not involve drug adsorption to the membrane, in contrary to UF, nor takes long time to perform as ED does. The method provides superior plasma clean-up, i.e., no interfering peaks exist from the plasma sample are seen in the chromatograms. It does not require skilled laboratory staff to perform the experiments, in contrary to other methods that require skilled personnel.