cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Edge instability in a chiral stripe domain under an electric current and skyrmion generation
Creators: name: Lin, Shi-Zeng
Publisher: name: arXiv
Year: 2015
Subjects: subject: Strongly Correlated Electrons (cond-mat.str-el)
subject: Materials Science (cond-mat.mtrl-sci)
subject: Pattern Formation and Solitons (nlin.PS)
subject: FOS: Physical sciences

description: Motivated by the recent experimental observations on the skyrmion creation by cutting chiral stripe domains under a current drive [Jiang {\emph{et al.}}, Science {\bf{349}}, 283 (2015)], we study the mechanism of skyrmion generation by simulating the dynamics of stripe domains. Our theory for skyrmion generation is based on the fact that there are two half skyrmions attached to the ends of a stripe domain. These half skyrmions move due to the coupling between the skyrmion topological charge and current. As a consequence, the stripe domain is bent or stretched depending on the direction of motion of the half skyrmions. For a large current, skyrmions are created by chopping the stripe domains via strong bending or stretching. Our theory provides an explanation to the experiments and is supported by the new experiments. Furthermore, we predict that skyrmions can also be generated using a Bloch stripe domain under a spin transfer torque which can be realized in B20 compounds.
description: 8 pages and 6 figures, movies can be found at http://journals.aps.org/prb/abstract/10.1103/PhysRevB.94.020402
Asplenium gilliesii Hook., Exot. Fl.
Creators: name: Kessler, Michael
name: Smith, Alan R.
Publisher: name: Zenodo
Year: 2018
Subjects: Biodiversity
Taxonomy
Plantae
Tracheophyta
Polypodiopsida
Polypodiales
Aspleniaceae
Asplenium
Asplenium gilliesii

description: Asplenium gilliesii Hook., Exot. Fl. 3: 208. 1827.
 Range:— Peru, Bolivia (CO, LP, OR, PO, SC, TA), and Argentina.
 Ecology:— Common; saxicolous, mainly in rock crevices, rarely terrestrial in high-elevation grassland and scrub; 2450–4250 m.
 Notes:— Very similar to Asplenium stoloniferum Bory from southern Africa and La Réunion, and perhaps conspecific (Moran & Smith 2001), but pending molecular evidence, we maintain them as separate species.
description: Published as part of Kessler, Michael & Smith, Alan R., 2018, Prodromus of a fern flora for Bolivia. XXIX. Aspleniaceae, pp. 259-280 in Phytotaxa 344 (3) on pages 259-280, DOI: 10.11646/phytotaxa.344.3.6, http://zenodo.org/record/13720625
Development of Patient-specific AAV Vectors After Neutralizing Antibody Selection for Enhanced Muscle Gene Transfer
Creators: name: Hirsch, Matthew
name: Li, Chengwen
name: Asokan, Aravind
name: Agbandje-Mckenna, Mavis
name: Li, Wuping
name: Mcphee, Scott
name: Tseng, Yu-Shan
name: Albright, Blake
name: Wu, Shuqing
name: Samulski, R. Jude
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2016

description: A major hindrance in gene therapy trials with adeno-associated virus (AAV) vectors is the presence of neutralizing antibodies (NAbs) that inhibit AAV transduction. In this study, we used directed evolution techniques in vitro and in mouse muscle to select novel NAb escape AAV chimeric capsid mutants in the presence of individual patient serum. AAV mutants isolated in vitro escaped broad patient-specific NAb activity but had poor transduction ability in vivo. AAV mutants isolated in vivo had enhanced NAb evasion from cognate serum and had high muscle transduction ability. More importantly, structural modeling identified a 100 amino acid motif from AAV6 in variable region (VR) III that confers this enhanced muscle tropism. In addition, a predominantly AAV8 capsid beta barrel template with a specific preference for AAV1/AAV9 in VR VII located at threefold symmetry axis facilitates NAb escape. Our data strongly support that chimeric AAV capsids composed of modular and nonoverlapping domains from various serotypes are capable of evading patient-specific NAbs and have enhanced muscle transduction.
Asplenium harpeodes Kunze, Linnaea
Creators: name: Kessler, Michael
name: Smith, Alan R.
Publisher: name: Zenodo
Year: 2018
Subjects: Biodiversity
Taxonomy
Plantae
Tracheophyta
Polypodiopsida
Polypodiales
Aspleniaceae
Asplenium
Asplenium harpeodes

description: Asplenium harpeodes Kunze, Linnaea 18: 329. 1844.
 Range:— Greater Antilles; southern Mexico to Bolivia (CH, CO, LP, SC) and southern Brazil.
 Ecology:— Very common; terrestrial, epiphytic, and less commonly saxicolous, in humid to semideciduous forests; (900) 1500–3600 m.
 Notes:— Very variable, probably constituting a complex of closely related species. In Bolivia, three distinct entities may be distinguished. Typical A. harpeodes has pinnae> 4 cm long with acute, often elongate apices, basal pinnae>1/2 the length of the longest pinna, acroscopic veins all simple or forked only on the basal lobes, acroscopic pinna lobes not overlapping the rachises, rhizome scales 0.4–0.7 mm wide, dark and lustrous petioles 1–2 mm thick, and epiphytic habit. A second form, perhaps referable to Brazilian A. jucundum Fée, has petioles 2–3 mm thick, acroscopic veins often forked well beyond the basal lobe, acroscopic pinna lobes often overlapping the rachises, and rhizome scales 0.8–1 mm wide. A third, probably undescribed form differs from typical A. harpeodes by pinnae <4 cm long with rounded to acuminate apices, basal pinnae often <1/2 the length of the longest pinnae, dull grayish to red-brown petioles, and usually terrestrial habit. However, numerous intermediate specimens occur and we therefore refrain from recognizing the three forms taxonomically, pending further research.
description: Published as part of Kessler, Michael & Smith, Alan R., 2018, Prodromus of a fern flora for Bolivia. XXIX. Aspleniaceae, pp. 259-280 in Phytotaxa 344 (3) on pages 259-280, DOI: 10.11646/phytotaxa.344.3.6, http://zenodo.org/record/13720625
Compactification tuning for nonlinear localized modes in sawtooth lattices
Creators: name: Johansson, Magnus
name: Naether, Uta
name: Vicencio, Rodrigo A.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Pattern Formation and Solitons (nlin.PS)
subject: Mesoscale and Nanoscale Physics (cond-mat.mes-hall)
subject: Optics (physics.optics)
subject: FOS: Physical sciences

description: We discuss the properties of nonlinear localized modes in sawtooth lattices, in the framework of a discrete nonlinear Schrödinger model with general on-site nonlinearity. Analytic conditions for existence of exact compact three-site solutions are obtained, and explicitly illustrated for the cases of power-law (cubic) and saturable nonlinearities. These nonlinear compact modes appear as continuations of linear compact modes belonging to a flat dispersion band. While for the linear system a compact mode exists only for one specific ratio of the two different coupling constants, nonlinearity may lead to compactification of otherwise non-compact localized modes for a range of coupling ratios, at some specific power. For saturable lattices, the compactification power can be tuned by also varying the nonlinear parameter. Introducing different on-site energies and anisotropic couplings yield further possibilities for compactness tuning. The properties of strongly localized modes are investigated numerically for cubic and saturable nonlinearities, and in particular their stability over large parameter regimes is shown. Since the linear flat band is isolated, its compact modes may be continued into compact nonlinear modes both for focusing and defocusing nonlinearities. Results are discussed in relation to recent realizations of sawtooth photonic lattices.
description: 12 pages with 11 figures
GD2-specific CAR T Cells Undergo Potent Activation and Deletion Following Antigen Encounter but can be Protected From Activation-induced Cell Death by PD-1 Blockade
Creators: name: Brown, Michael P.
name: Christo, Susan N.
name: Lewis, Ian D.
name: Dotti, Gianpietro
name: Brenner, Malcolm K.
name: Hayball, John D.
name: Yu, Wenbo
name: Gargett, Tessa
name: Yvon, Eric S
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2016

description: Chimeric antigen receptor (CAR) T cells have shown great promise in the treatment of hematologic malignancies but more variable results in the treatment of solid tumors and the persistence and expansion of CAR T cells within patients has been identified as a key correlate of antitumor efficacy. Lack of immunological “space”, functional exhaustion, and deletion have all been proposed as mechanisms that hamper CAR T-cell persistence. Here we describe the events following activation of third-generation CAR T cells specific for GD2. CAR T cells had highly potent immediate effector functions without evidence of functional exhaustion in vitro, although reduced cytokine production reversible by PD-1 blockade was observed after longer-term culture. Significant activation-induced cell death (AICD) of CAR T cells was observed after repeated antigen stimulation, and PD-1 blockade enhanced both CAR T-cell survival and promoted killing of PD-L1+ tumor cell lines. Finally, we assessed CAR T-cell persistence in patients enrolled in the CARPETS phase 1 clinical trial of GD2-specific CAR T cells in the treatment of metastatic melanoma. Together, these data suggest that deletion also occurs in vivo and that PD-1-targeted combination therapy approaches may be useful to augment CAR T-cell efficacy and persistence in patients.
Nanoparticle Delivered VEGF-A siRNA Enhances Photodynamic Therapy for Head and Neck Cancer Treatment
Creators: name: Lecaros, Rumwald Leo G.
name: Hsu, Yih-Chih
name: Huang, Leaf
name: Lee, Tsai-Chia
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2016

description: Photodynamic therapy (PDT) is believed to promote hypoxic conditions to tumor cells leading to overexpression of angiogenic markers such as vascular endothelial growth factor (VEGF). In this study, PDT was combined with lipid–calcium–phosphate nanoparticles (LCP NPs) to deliver VEGF-A small interfering RNA (siVEGF-A) to human head and neck squamous cell carcinoma (HNSCC) xenograft models. VEGF-A were significantly decreased for groups treated with siVEGF-A in human oral squamous cancer cell (HOSCC), SCC4 and SAS models. Cleaved caspase-3 and in situ TdT-mediated dUTP nick-end labeling assay showed more apoptotic cells and reduced Ki-67 expression for treated groups compared to phosphate buffered saline (PBS) group. Indeed, the combined therapy showed significant tumor volume decrease to ~70 and ~120% in SCC4 and SAS models as compared with untreated PBS group, respectively. In vivo toxicity study suggests no toxicity of such LCP NP delivered siVEGF-A. In summary, results suggest that PDT combined with targeted VEGF-A gene therapy could be a potential therapeutic modality to achieve enhanced therapeutic outcome for HNSCC.
Data Driven Optical Cool Dwarf Chemistry & the Giant Planet–[Fe/H] Correlation
Creators: name: Rains, Adam D.
name: Nordlander, Thomas
name: Casey, Andrew R.
name: Žerjal, Maruša
name: Ireland, Michael J.
name: Bessell, Michael S.
Publisher: name: Zenodo
Year: 2023
Subjects: cool dwarf stars
low-mass stars
spectroscopy
fundamental parameters
data driven
stellar chemistry

description: Detailed chemical studies of Solar-type stars have long been routine in stellar astrophysics, making possible studies in Galactic chemodynamics and exoplanet demographics. However, a similar understanding of the chemistry of M and late-K dwarfs—the most common stars in the Galaxy and most likely to host planets—has been greatly hampered by the complex molecular chemistry of their cool atmospheres. Here we present a new implementation of the Cannon, a data-driven model widely used in stellar astrophysics, developed for low–medium resolution optical (400–700nm) cool dwarf spectra. Our novel four parameter implementation in Teff, log g, [Fe/H], and [Ti/H] models both label uncertainties and missing labels, and is trained on 121 cool dwarf benchmarks—21 of which have literature elemental abundances measured from a warmer binary companion. Under leave-one-out cross-validation, we recover Teff, [Fe/H], and [Ti/H] with precisions of 2%, ±0.12 dex, and ±0.09 dex respectively, a precision which allows insight into the giant planet–[Fe/H] correlation for our sample of 65 TESS candidate planet hosts. Our work highlights the importance of precisely known benchmark systems; the promise of data-driven models for chemical analysis of the rich, but challenging to model, optical spectra of cool stars; and the utility of both in studying exoplanet demographics in the era of TESS and Gaia.
description: {"references": ["Rains, A. D. et al. (2021), MNRAS, 504, 5788"]}
Novel Conserved-region T-cell Mosaic Vaccine With High Global HIV-1 Coverage Is Recognized by Protective Responses in Untreated Infection
Creators: name: Ondondo, Beatrice
name: Abdul-Jawad, Sultan
name: Clutton, Genevieve
name: Takiguchi, Masafumi
name: Wee, Edmund G.-T.
name: Murakoshi, Hayato
name: McMichael, Andrew J
name: Oka, Shinichi
name: Korber, Bette
name: Gatanaga, Hiroyuki
name: Hanke, Tom
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2016

description: An effective human immunodeficiency virus type 1 (HIV-1) vaccine is the best solution for halting the acquired immune deficiency syndrome epidemic. Here, we describe the design and preclinical immunogenicity of T-cell vaccine expressing novel immunogens tHIVconsvX, vectored by DNA, simian (chimpanzee) adenovirus, and poxvirus modified vaccinia virus Ankara (MVA), a combination highly immunogenic in humans. The tHIVconsvX immunogens combine the three leading strategies for elicitation of effective CD8+ T cells: use of regions of HIV-1 proteins functionally conserved across all M group viruses (to make HIV-1 escape costly on viral fitness), inclusion of bivalent complementary mosaic immunogens (to maximize global epitope matching and breadth of responses, and block common escape paths), and inclusion of epitopes known to be associated with low viral load in infected untreated people (to induce field-proven protective responses). tHIVconsvX was highly immunogenic in two strains of mice. Furthermore, the magnitude and breadth of CD8+ T-cell responses to tHIVconsvX-derived peptides in treatment-naive HIV-1+ patients significantly correlated with high CD4+ T-cell count and low viral load. Overall, the tHIVconsvX design, combining the mosaic and conserved-region approaches, provides an indisputably better coverage of global HIV-1 variants than previous T-cell vaccines. These immunogens delivered in a highly immunogenic framework of adenovirus prime and MVA boost are ready for clinical development.
title: Early Detection of SARS-CoV-2 Omicron BA.4 and BA.5 in German Wastewater
Creators: name: Wilhelm, Alexander
name: Agrawal, Shelesh
name: Schoth, Jens
name: Meinert-Berning, Christina
name: Bastian, Daniel
name: Orschler, Laura
name: Ciesek, Sandra
name: Teichgräber, Burkhard
name: Wintgens, Thomas
name: Lackner, Susanne
name: Weber, Frank-Andreas
name: Widera, Marek
Publisher: name: MDPI
Year: 2022

description: Wastewater-based SARS-CoV-2 epidemiology (WBE) has been established as an important tool to support individual testing strategies. The Omicron sub-variants BA.4/BA.5 have spread globally, displacing the preceding variants. Due to the severe transmissibility and immune escape potential of BA.4/BA.5, early monitoring was required to assess and implement countermeasures in time. In this study, we monitored the prevalence of SARS-CoV-2 BA.4/BA.5 at six municipal wastewater treatment plants (WWTPs) in the Federal State of North Rhine-Westphalia (NRW, Germany) in May and June 2022. Initially, L452R-specific primers/probes originally designed for SARS-CoV-2 Delta detection were validated using inactivated authentic viruses and evaluated for their suitability for detecting BA.4/BA.5. Subsequently, the assay was used for RT-qPCR analysis of RNA purified from wastewater obtained twice a week at six WWTPs. The occurrence of L452R carrying RNA was detected in early May 2022, and the presence of BA.4/BA.5 was confirmed by variant-specific single nucleotide polymorphism PCR (SNP-PCR) targeting E484A/F486V and NGS sequencing. Finally, the mutant fractions were quantitatively monitored by digital PCR, confirming BA.4/BA.5 as the majority variant by 5 June 2022. In conclusion, the successive workflow using RT-qPCR, variant-specific SNP-PCR, and RT-dPCR demonstrates the strength of WBE as a versatile tool to rapidly monitor variants spreading independently of individual test capacities.
HVAC for non-sterile Pharmaceutical Products: Design, Qualification and Maintenance
Creators: name: Rahman, Tareq
name: Pawar, Amalesh Singh
Publisher: name: figshare
Year: 2022
Subjects: subject: 111504 Pharmaceutical Sciences
subject: FOS: Clinical medicine

description: Abstract
 Heating, ventilation and air-conditioning (HVAC) system plays an important role to ensure the manufacture of quality pharmaceutical products. In this article, design consideration, qualification requirements and maintenance of HVAC has been discussed. Regulatory guidelines and risk-based approaches are considered. Pharmaceutical company should design HVAC, qualify and managed the HVAC system based on risk assessment.
Kanashiro-Takeuchi_Efficacy of GHRH_Supplemental_AJP Heart
Creators: name: Kanashiro-Takeuchi, Rosemeire
name: Takeuchi, Lauro
name: Dulce, Raul
name: Kazmierczak, Katarzyna
name: Balkan, Wayne
name: Cai, Rhenzi
name: Sha, Wei
name: Schally, Andrew
name: M. Hare, Joshua
Publisher: name: figshare
Year: 2022
Subjects: Cardiology

description: Supplemental Data: Material and Methods Supplemental Figures: S1-S4 Supplemental Tables: S1-S6
Production of Recombinant Adeno-associated Virus Vectors Using Suspension HEK293 Cells and Continuous Harvest of Vector From the Culture Media for GMP FIX and FLT1 Clinical Vector
Creators: name: Samulski, Richard Jude
name: Soltys, Stephen M.
name: Grieger, Joshua C.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2016

description: Adeno-associated virus (AAV) has shown great promise as a gene therapy vector in multiple aspects of preclinical and clinical applications. Many developments including new serotypes as well as self-complementary vectors are now entering the clinic. With these ongoing vector developments, continued effort has been focused on scalable manufacturing processes that can efficiently generate high-titer, highly pure, and potent quantities of rAAV vectors. Utilizing the relatively simple and efficient transfection system of HEK293 cells as a starting point, we have successfully adapted an adherent HEK293 cell line from a qualified clinical master cell bank to grow in animal component-free suspension conditions in shaker flasks and WAVE bioreactors that allows for rapid and scalable rAAV production. Using the triple transfection method, the suspension HEK293 cell line generates greater than 1 × 105 vector genome containing particles (vg)/cell or greater than 1 × 1014 vg/l of cell culture when harvested 48 hours post-transfection. To achieve these yields, a number of variables were optimized such as selection of a compatible serum-free suspension media that supports both growth and transfection, selection of a transfection reagent, transfection conditions and cell density. A universal purification strategy, based on ion exchange chromatography methods, was also developed that results in high-purity vector preps of AAV serotypes 1–6, 8, 9 and various chimeric capsids tested. This user-friendly process can be completed within 1 week, results in high full to empty particle ratios (>90% full particles), provides postpurification yields (>1 × 1013 vg/l) and purity suitable for clinical applications and is universal with respect to all serotypes and chimeric particles. To date, this scalable manufacturing technology has been utilized to manufacture GMP phase 1 clinical AAV vectors for retinal neovascularization (AAV2), Hemophilia B (scAAV8), giant axonal neuropathy (scAAV9), and retinitis pigmentosa (AAV2), which have been administered into patients. In addition, we report a minimum of a fivefold increase in overall vector production by implementing a perfusion method that entails harvesting rAAV from the culture media at numerous time-points post-transfection.
Serial Activation of the Inducible Caspase 9 Safety Switch After Human Stem Cell Transplantation
Creators: name: Naik, Swati
name: Dakhova, Olga
name: Zhou, Xiaoou
name: Dotti, Gianpietro
name: Brenner, Malcolm K.
name: Heslop, Helen E.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2016

description: Activation of the inducible caspase 9 (iC9) safety gene by a dimerizing drug (chemical inducer of dimerization (CID) AP1903) effectively resolves the symptoms and signs of graft-versus-host disease (GvHD) in haploidentical stem cell transplant (HSCT) recipients. However, after CID treatment, 1% of iC9-T cells remain and can regrow over time; although these resurgent T cells do not cause recurrent GvHD, it remains unclear whether repeat CID treatments are a safe and feasible way to further deplete residual gene-modified T cells should any other adverse effects associated with them occur. Here, we report a patient who received an infusion of haploidentical iC9-T cells after HSCT and subsequently received three treatments with AP1903. There was a mild (grade 2) and transient pancytopenia following each AP1903 administration but no non-hematological toxicity. Ninety five percent of circulating iC9-T cells (CD3+CD19+) were eliminated after the first AP1903 treatment. Three months later, the residual cells had expanded more than eightfold and had a lower level of iC9 expression. Each repeated AP1903 administration eliminated a diminishing percentage of the residual repopulating cells, but elimination could be enhanced by T-cell activation. These data support the safety and efficiency of repeated CID treatments for persistent or recurring toxicity from T-cell therapies.
Confirmation of the proto-brown dwarf SSTB213-J041757B and discovery of a nearby new candidate with ALMA and CARMA
Creators: name: Palau, Aina
name: Durán-García, Mariano
name: Fernández-López, Manuel
name: Zapata, Luis A.
name: Morata, Oscar
name: de Gregorio-Monsalvo, Itziar
name: Barrado, David
name: Huelamo, Nuria
name: Bayo, Amelia
name: Ruiz, Maria Teresa
name: Bouy, Herve
name: Ribas, Alvaro
name: Morales-Calderon, Maria
name: Rodriguez, Luis Felipe
Publisher: name: Zenodo
Year: 2023
Subjects: brown dwarfs
substellar objects
Radio Astronomy

description: How Brown Dwarfs (BDs) form is still an unresolved question. In order to shed light into the different possible mechanisms for BD formation, it is essential to identify bona-fide BDs in their earliest evolutionary stages, the so-called proto-BDs, equivalent to objects in the Class 0 and Class I stages of young stellar objects. We conducted CARMA observations at 3 and 1 mm and several transitions, along with ALMA observations at 1 mm and CO(2-1) towards a debated proto-BD candidate in the Taurus molecular cloud. Both ALMA and CARMA observations reveal signs of outflow emission driven by the proto-BD candidate. In addition, CARMA allowed to discover a new proto-BD candidate about 50 arcsec to the northeast, that also seems to drive a molecular outflow. A comparison of the outflow momentum rate of these two proto-BD candidates with those of well-known young stellar objects indicates that this parameter falls at the expected relation according to their lower luminosity. Overall, these observations allow to add two proto-BDs in the short list of proto-BDs known to date, and suggest that BDs seem to form as a scaled-down version of low-mass stars. Funded by the UNAM-PAPIIT IN111421 grant.
description: {"references": ["Barrado, D. et al. (2009), A&A, 508, 859", "Palau, A. et al. (2012), MNRAS, 424, 2778"]}
Inducible Caspase-9 Selectively Modulates the Toxicities of CD19-Specific Chimeric Antigen Receptor-Modified T Cells
Creators: name: Savoldo, Barbara
name: West, John
name: Dotti, Gianpietro
name: Diaconu, Iulia
name: Ballard, Brandon
name: Zhang, Ming
name: Chen, Yuhui
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2017

description: Immunotherapy with T cells expressing the chimeric antigen receptor (CAR) specific for the CD19 antigen (CD19.CAR-Ts) is a very effective treatment in B cell lymphoid malignancies. However, B cell aplasia and cytokine release syndrome (CRS) secondary to the infusion of CD19.CAR-Ts remain significant drawbacks. The inclusion of safety switches into the vector encoding the CAR is seen as the safest method to terminate the effects of CD19.CAR-Ts in case of severe toxicities or after achieving long-term sustained remissions. By contrast, the complete elimination of CD19.CAR-Ts when CRS occurs may jeopardize clinical responses as CRS and antitumor activity seem to concur. We have demonstrated, in a humanized mouse model, that the inducible caspase-9 () safety switch can eliminate CD19.CAR-Ts in a dose-dependent manner, allowing either a selective containment of CD19.CAR-T expansion in case of CRS or complete deletion on demand granting normal B cell reconstitution.
Improvements in Engine Performance Simulations and Integrated Engine Thermal Modeling
Creators: name: Ponkshe, Aishwarya Vinod
Publisher: name: Purdue University Graduate School
Year: 2023
Subjects: Mechanical engineering not elsewhere classified
Automotive engineering not elsewhere classified
Automotive combustion and fuel engineering
Fluid mechanics and thermal engineering not elsewhere classified

description: One of the major challenges in the field of internal combustion engines is keeping up with the advancements in electrification and hybridization. Automakers are striving to design environment – friendly and highly efficient engines to meet stringent emission standards worldwide. Improving engine efficiency and reducing heat losses are critical aspects of this development. Therefore, accurate heat transfer prediction capabilities play a vital role in engine design process. Current methods rely on computationally intensive 3D numerical analyses, there is a growing interest in reliable simplified models. In this study, a 1D diesel engine model featuring predictive combustion was integrated with a detailed finite element thermal primitive based on the 3D meshing feature available in GT Suite. Coolant and oil hydraulic circuits were incorporated in the model. The model proves to be an effective means to assess the impact on heat rejection and engine heat distribution given by an engine calibration and operating conditions. This work also contributes to the advancement of virtual IC engine development methods by focusing on the design and tuning of complex engine system models using GT Power for accurate prediction of engine performance. The current processes in engine simulations are assessed to identify sources of errors and opportunities for improvements. The methods discussed in this work include isolated sub system level calibration and model evolution specifically address the issue of identifying noise factors and issues in smaller parts. Additionally, the study aims on improving the model’s trustworthiness by computing 1st law sanity checks, replicating real-life compressor map calculations and refining GT’s existing global convergence criteria.
Stellar magnetic fields for star-planet interactions: HD 219134 and 55 Cnc
Creators: name: Folsom, Colin P.
name: Vidotto, Aline A.
name: Fossati, Luca
Publisher: name: Zenodo
Year: 2023

description: A promising avenue for investigating star-planet interactions (SPI) is the impact of stellar winds on exoplanets. Stellar winds cannot usually be directly observed, but realistic predictions can be made. These predictions rely on observations to constrain the large-scale stellar magnetic field geometry, which sculpts the wind. An additional rare but interesting mode of SPI is a more direct magnetic interaction, possible for planets orbiting inside the Alfven radius of the star. Here we present observations of two stars with close in exoplanets, 55 Cnc and HD 219134. We derive magnetic maps using Zeeman Doppler Imaging and wind models using MHD simulations. For HD 219134 b, the strong stellar wind impacting the planet may produce observable effects through sputtering of material from the planet's surface. For 55 Cnc e, the planet appears to orbit inside the Alfven surface of the wind, allowing for magnetic SPI, such as reconnection generating bright points on the star. (Slides from a presentation in the Manifestations of Star-Planet Interactions splinter session)
description: {"references": ["Folsom, C. P. et al. (2018), MNRAS, 481, 5286", "Vidotto, A. A. et al. (2018) MNRAS, 481, 5296", "Folsom, C. P. et al. (2020), A&A, 633, A48"]}
title: Detailed Characterization of Small Extracellular Vesicles from Different Cell Types Based on Tetraspanin Composition by ExoView R100 Platform
Creators: name: Breitwieser, Kai
name: Koch, Leon F.
name: Tertel, Tobias
name: Proestler, Eva
name: Burgers, Luisa D.
name: Lipps, Christoph
name: Adjaye, James
name: Fürst, Robert
name: Giebel, Bernd
name: Saul, Meike J.
Publisher: name: MDPI
Year: 2022

description: Small extracellular vesicles (sEV) hold enormous potential as biomarkers, drug carriers, and therapeutic agents. However, due to previous limitations in the phenotypic characterization of sEV at the single vesicle level, knowledge of cell type-specific sEV signatures remains sparse. With the introduction of next-generation sEV analysis devices, such as the single-particle interferometric reflectance imaging sensor (SP-IRIS)-based ExoView R100 platform, single sEV analyses are now possible. While the tetraspanins CD9, CD63, and CD81 were generally considered pan-sEV markers, it became clear that sEV of different cell types contain several combinations and amounts of these proteins on their surfaces. To gain better insight into the complexity and heterogeneity of sEV, we used the ExoView R100 platform to analyze the CD9/CD63/CD81 phenotype of sEV released by different cell types at a single sEV level. We demonstrated that these surface markers are sufficient to distinguish cell-type-specific sEV phenotypes. Furthermore, we recognized that tetraspanin composition in some sEV populations does not follow a random pattern. Notably, the tetraspanin distribution of sEV derived from mesenchymal stem cells (MSCs) alters depending on cell culture conditions. Overall, our data provide an overview of the cell-specific characteristics of sEV populations, which will increase the understanding of sEV physiology and improve the development of new sEV-based therapeutic approaches.
Oral Tolerance Induction in Hemophilia B Dogs Fed with Transplastomic Lettuce
Creators: name: Sherman, Alexandra
name: Merricks, Elizabeth P.
name: Häger, Mattias
name: Daniell, Henry
name: Wiinberg, Bo
name: Zhang, Bei
name: Raymer, Robin
name: Su, Jin
name: Nichols, Timothy C.
name: Perrin, George Q.
name: Herzog, Roland W.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2017

description: Anti-drug antibodies in hemophilia patients substantially complicate treatment. Their elimination through immune tolerance induction (ITI) protocols poses enormous costs, and ITI is often ineffective for factor IX (FIX) inhibitors. Moreover, there is no prophylactic ITI protocol to prevent anti-drug antibody (ADA) formation. Using general immune suppression is problematic. To address this urgent unmet medical need, we delivered antigen bioencapsulated in plant cells to hemophilia B dogs. Commercial-scale production of CTB-FIX fusion expressed in lettuce chloroplasts was done in a hydroponic facility. CTB-FIX (∼1 mg/g) in lyophilized cells was stable with proper folding, disulfide bonds, and pentamer assembly after 30-month storage at ambient temperature. Robust suppression of immunoglobulin G (IgG)/inhibitor and IgE formation against intravenous FIX was observed in three of four hemophilia B dogs fed with lyophilized lettuce cells expressing CTB-FIX. No side effects were detected after feeding CTB-FIX-lyophilized plant cells for >300 days. Coagulation times were markedly shortened by intravenous FIX in orally tolerized treated dogs, in contrast to control dogs that formed high-titer antibodies to FIX. Commercial-scale production, stability, prolonged storage of lyophilized cells, and efficacy in tolerance induction in a large, non-rodent model of human disease offer a novel concept for oral tolerance and low-cost production and delivery of biopharmaceuticals.
Asplenium incurvatum Fee, Crypt. Vasc.
Creators: name: Kessler, Michael
name: Smith, Alan R.
Publisher: name: Zenodo
Year: 2018
Subjects: Biodiversity
Taxonomy
Plantae
Tracheophyta
Polypodiopsida
Polypodiales
Aspleniaceae
Asplenium
Asplenium incurvatum

description: Asplenium incurvatum Fée, Crypt. Vasc. Brésil 1: 69, t. 18, f. 1. 1869.
 Range:— Andes from Venezuela to Bolivia (BE, CO, LP, SC); southeastern Brazil.
 Ecology:— Fairly common; epiphytic, terrestrial, and saxicolous in humid forests, probably prefers limestone and sandstone outcrops; 850–3050 m.
 Notes:— The Andean populations attributed by us to this species may not be conspecific with those from southeastern Brazil, the source of the type. Jonas (2011) did not find any consistent molecular or morphological traits (besides rhizome scale length) to separate this species from A. achalense.
description: Published as part of Kessler, Michael & Smith, Alan R., 2018, Prodromus of a fern flora for Bolivia. XXIX. Aspleniaceae, pp. 259-280 in Phytotaxa 344 (3) on pages 259-280, DOI: 10.11646/phytotaxa.344.3.6, http://zenodo.org/record/13720625
Strongly driven nonlinear quantum optics in microring resonators
Creators: name: Vernon, Z.
name: Sipe, J. E.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Quantum Physics (quant-ph)
subject: Optics (physics.optics)
subject: FOS: Physical sciences

description: We present a detailed analysis of strongly driven spontaneous four-wave mixing in a lossy integrated microring resonator side-coupled to a channel waveguide. A nonperturbative, analytic solution within the undepleted pump approximation is developed for a cw pump input of arbitrary intensity. In the strongly driven regime self- and cross-phase modulation, as well as multi-pair generation, lead to a rich variety of power-dependent effects; the results are markedly different than in the low power limit. The photon pair generation rate, single photon spectrum, and joint spectral intensity (JSI) distribution are calculated. Splitting of the generated single photon spectrum into a doublet structure associated with both pump detuning and cross-phase modulation is predicted, as well as substantial narrowing of the generated signal and idler bandwidths associated with the onset of optical parametric oscillation at intermediate powers. Both the correlated and uncorrelated contributions to the JSI are calculated, and for sufficient powers the uncorrelated part of the JSI is found to form a quadruplet structure. The pump detuning is found to play a crucial role in all of these phenomena, and a critical detuning is identified which divides the system behaviour into distinct regimes, as well as an optimal detuning strategy which preserves many of the low-power characteristics of the generated photons for arbitrary input power.
description: 18 pages, 10 figures
Uniform measure density condition and game regularity for tug-of-war games
Creators: name: Heino, Joonas
Publisher: name: arXiv
Year: 2015
Subjects: subject: Probability (math.PR)
subject: Analysis of PDEs (math.AP)
subject: FOS: Mathematics
subject: 91A15, 60G50, 35J92

description: We show that a uniform measure density condition implies game regularity for all $2
Cost-Benefit Decision Making for Dataset FAIRification in Pharmaceutical R&D
Creators: name: Goble, Carole
name: Alharbi, Ebtisam
name: Juty, Nick
name: Jay, Caroline
name: Skeva, Rigina
Publisher: name: Zenodo
Year: 2022
Subjects: FAIR Data; Pharmaceutical R&D; decision making; Cost-benefit analysis; FAIRplus

description: The FAIR (findable, accessible, interoperable and reusable) principles of scientific data management and stewardship are aimed at facilitating data reuse at scale by both humans and machines. Research and development (R&D) in the pharmaceutical industry is becoming increasingly data driven, but managing its data assets according to FAIR principles remains a costly and challenging endeavour. But what are the associated costs and benefits, and how are decisions supported about the value of the FAIRification of existing datasets in pharmaceutical R&D? A decision-making framework called FAIR-Decide uses the application of business analysis techniques (cost-benefit and multi-criteria analyses) in assessing estimated costs and expected benefits. The framework and associated tool were developed through workshops and focus groups with a number of Pharma R&D stakeholders associated with the IMI FAIRplus project.
description: presented at BioITWorld Europe, 18-19 Oct 2022, Berlin, Germany by Carole Goble
description: {"references": ["Ebtisam Alharbi, Rigina Skeva, Nick Juty, Caroline Jay, Carole Goble; Exploring the Current Practices, Costs and Benefits of FAIR Implementation in Pharmaceutical Research and Development: A Qualitative Interview Study. Data Intelligence 2021; 3 (4): 507\u2013527. doi: https://doi.org/10.1162/dint_a_00109", "Reilly, D., Alharbi, E., Gadiya, Y., Henderson, D., Zaliani, A., Delfin-Rossaro, A., ... & Gribbon, P. (2022). Selection of datasets for FAIRification in Drug Discovery and Development: Which, Why, and How?. Drug discovery today"]}
title: Spontanes Hämatom des Larynx unter Rivaroxaban nach Stimmbelastung
Creators: name: Tache, Petrut
name: Henschke, Frank
name: Folz, Benedikt J.
Publisher: name: German Medical Science GMS Publishing House
Year: 2019
Subjects: subject: 610 Medical sciences; Medicine

description: Einleitung: Fallbericht über ein Hämatom des Larynx unter Rivaroxabantherapie bei Vorhofflim­mern. Kasuistik: Wir berichten über den Fall eines Patienten mit Heiserkeit seit 6 Wochen ohne Dyspha­gie oder Dyspnoe sowie keine B-Symptomatik, kein Nikotinabusus. Unter Rivaroxaban[zum vollständigen Text gelangen Sie über die oben angegebene URL]
description: Jahrestagung der Vereinigung Westdeutscher Hals-Nasen-Ohren-Ärzte
title: Безопасность жизнедеятельности. Вредные факторы производственной среды
Creators: name: ELS IPR BOOKS
Publisher: name: Профобразование
Year: 2024

description: В учебном пособии рассмотрены теоретические основы вредных факторов производственной среды, вопросы производственного микроклимата, освещения, шума и вибраций. Представлены методические указания для успешного выполнения лабораторных работ. Учебное пособие предназначено для студентов всех профессий и специальностей среднего профессионального образования, учебными планами которых предусмотрено изучение дисциплины «Безопасность жизнедеятельности».
Asplenium juglandifolium Lam., Encycl.
Creators: name: Kessler, Michael
name: Smith, Alan R.
Publisher: name: Zenodo
Year: 2018
Subjects: Biodiversity
Taxonomy
Plantae
Tracheophyta
Polypodiopsida
Polypodiales
Aspleniaceae
Asplenium
Asplenium juglandifolium

description: Asplenium juglandifolium Lam., Encycl. 2: 307. 1786.
 Range:— Antilles; southern Mexico to Bolivia (BE, CO, LP, PA, SC) and Brazil.
 Ecology:— Fairly common; epiphytic in humid forests; 100–1800 m, to 2000 m elsewhere. Typically found on thick tree trunks (Zotz 2007).
description: Published as part of Kessler, Michael & Smith, Alan R., 2018, Prodromus of a fern flora for Bolivia. XXIX. Aspleniaceae, pp. 259-280 in Phytotaxa 344 (3) on pages 259-280, DOI: 10.11646/phytotaxa.344.3.6, http://zenodo.org/record/13720625
How to compute Spectral Lines of 3D Atmosphere Simulations from 1D Data
Creators: name: Trampedach, Regner
name: Amarsi, Anish
name: Bigot, Lionel
name: Slumstrup, Ditte
Publisher: name: Zenodo
Year: 2023
Subjects: Stellar: spectra, stellar: convection, stellar: atmospheres

description: We present a method for performing spectral synthesis based on 1D averages of 3D simulations of convective stellar atmospheres, but recovering the detailed, asymmetric profiles of the full 3D calculation. This approach is not constrained to a particular set of lines, and a favorite line list can indeed by chosen freely by the spectroscopist." 3D-quality lines for the price of 1D calculations will be invaluable for producing high fidelity atmospheric parameters ($T_{\rm eff}$ and $\log g$) and abundances from large-scale stellar spectroscopic surveys like RAVE, GALAH and SDSS/APOGEE. It will likewise support the objectives of the GAIA and TESS missions, as well as the past Kepler/K2 and future PLATO missions, and bring our view of exo-planet systems, asteroseismology targets and Galactic archaeology into sharper focus.
description: Funded by NASA grant 80NSSC20K0543.
description: {"references": ["Amarsi et al. 2016, MNRAS, 455, 3735", "Gardiner et al. 1999, A&A, 347, 876", "Slumstrup et al. 2019, A&A, 622, A111", "Trampedach et al. 2014, MNRAS, 445, 4366"]}
title: Digitale Bereitstellung von Veranstaltungsaufzeichnungen – Ersatz oder Ergänzung?
Creators: name: Dombrowski, Tobias
name: Dazert, Stefan
name: Volkenstein, Stefan
Publisher: name: German Medical Science GMS Publishing House
Year: 2019
Subjects: subject: 610 Medical sciences; Medicine

description: Einleitung: Die digitale Bereitstellung von aufgezeichneten Vorlesungen oder Veranstaltungen ist ein viel diskutiertes Element digitaler Lehrstrategien. Mit der zunehmenden zentralen Etablierung von automatisierten Aufzeichnungssystemen durch die Universitäten sind sowohl die Kosten, als auch der[zum vollständigen Text gelangen Sie über die oben angegebene URL]
description: Jahrestagung der Vereinigung Westdeutscher Hals-Nasen-Ohren-Ärzte
Construction of a hybrid β-hexosaminidase subunit capable of forming stable homodimers that hydrolyze GM2 ganglioside in vivo
Creators: name: Tropak, Michael B
name: Karumuthil-Melethil, Subha
name: Walia, Jagdeep S
name: Mark, Brian L
name: Mahuran, Don
name: Thompson, Patrick
name: Wakarchuk, Warren
name: Yonekawa, Sayuri
name: Gray, Steven J
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2016

description: Tay-Sachs or Sandhoff disease result from mutations in either the evolutionarily related HEXA or HEXB genes encoding respectively, the α- or β-subunits of β-hexosaminidase A (HexA). Of the three Hex isozymes, only HexA can interact with its cofactor, the GM2 activator protein (GM2AP), and hydrolyze GM2 ganglioside. A major impediment to establishing gene or enzyme replacement therapy based on HexA is the need to synthesize both subunits. Thus, we combined the critical features of both α- and β-subunits into a single hybrid µ-subunit that contains the α-subunit active site, the stable β-subunit interface and unique areas in each subunit needed to interact with GM2AP. To facilitate intracellular analysis and the purification of the µ-homodimer (HexM), CRISPR-based genome editing was used to disrupt the HEXA and HEXB genes in a Human Embryonic Kidney 293 cell line stably expressing the µ-subunit. In association with GM2AP, HexM was shown to hydrolyze a fluorescent GM2 ganglioside derivative both in cellulo and in vitro. Gene transfer studies in both Tay-Sachs and Sandhoff mouse models demonstrated that HexM expression reduced brain GM2 ganglioside levels.
Metrics and causality on Moyal planes
Creators: name: Franco, Nicolas
name: Wallet, Jean-Christophe
Publisher: name: arXiv
Year: 2015
Subjects: subject: Mathematical Physics (math-ph)
subject: High Energy Physics - Theory (hep-th)
subject: FOS: Physical sciences
subject: 58B34, 54E35, 53C50, 54F05

description: Metrics structures stemming from the Connes distance promote Moyal planes to the status of quantum metric spaces. We discuss this aspect in the light of recent developments, emphasizing the role of Moyal planes as representative examples of a recently introduced notion of quantum (noncommutative) locally compact space. We move then to the framework of Lorentzian noncommutative geometry and we examine the possibility of defining a notion of causality on Moyal plane, which is somewhat controversial in the area of mathematical physics. We show the actual existence of causal relations between the elements of a particular class of pure (coherent) states on Moyal plane with related causal structure similar to the one of the usual Minkowski space, up to the notion of locality.
description: 33 pages. Improved version; a summary added at the end of the introduction, misprints corrected. Version to appear in Contemporary Mathematics
title: Веб-технологии
Creators: name: ELS IPR BOOKS
Publisher: name: Профобразование
Year: 2024

description: В практикуме представлены практические работы по веб-технологиям. Рассмотрены общие принципы работы клиентских, серверных языков и технологий в интернете, основные протоколы, необходимые для создания и работы веб-приложений. Приведены основные понятия и определения способов передачи по сети интернет, веб-технологий, предложены практические работы для изучения характеристик и классификация IT-сервисов и контента организаций. Практикум предназначен для студентов укрупненной группы специальностей среднего профессионального образования «Информатика и вычислительная техника», изучающих дисциплины «Основы веб-дизайна», «Веб-программирование»».
A Geometrical Stability Condition for Compressed Sensing
Creators: name: Flinth, Axel
Publisher: name: arXiv
Year: 2015
Subjects: subject: Functional Analysis (math.FA)
subject: FOS: Mathematics
subject: 52A20, 90C25 (Primary), 94A12 (Secondary)

description: During the last decade, the paradigm of compressed sensing has gained significant importance in the signal processing community. While the original idea was to utilize sparsity assumptions to design powerful recovery algorithms of vectors $x \in \mathbb{R}^d$, the concept has been extended to cover many other types of problems. A noteable example is low-rank matrix recovery. Many methods used for recovery rely on solving convex programs. A particularly nice trait of compressed sensing is its geometrical intuition. In recent papers, a classical optimality condition has been used together with tools from convex geometry and probability theory to prove beautiful results concerning the recovery of signals from Gaussian measurements. In this paper, we aim to formulate a geometrical condition for stability and robustness, i.e. for the recovery of approximately structured signals from noisy measurements. We will investigate the connection between the new condition with the notion of restricted singular values, classical stability and robustness conditions in compressed sensing, and also to important geometrical concepts from complexity theory. We will also prove the maybe somewhat surprising fact that for many convex programs, exact recovery of a signal $x_0$ immediately implies some stability and robustness when recovering signals close to $x_0$.
description: Changes in v2: Some typos and minor errors were corrected. The statements of the paper are the same up to the values of some constants. Also, the terms "stable" and "robust" were interchanged. Changes in v3: Peer reviewed version. Section 2.1 was added, and Section 3.3 was completely revised. Also, further typos etc. were corrected. Changes in v4: An issue with references not appearing was fixed
Hu_Supplementary材料
Creators: name: Hu, Xinqun
Publisher: name: figshare
Year: 2023
Subjects: classificationCode: 420311
subject: Health systems

description: 尿白蛋白与肌酐比值 （UACR） 是广泛使用的白蛋白尿指标，对不良心血管事件具有预测价值。 评估UACR与2型糖尿病（T2DM）患者发生重大心血管不良事件（MACEs）的风险和总死亡率之间的相关性。这项事后分析包括来自控制糖尿病心血管风险行动（ACCORD）研究和ACCORD随访研究的10,171名参与者，以及基线UACR数据。计算每个UACR测量的自然对数（ln）。进行单变量和多变量Cox比例风险回归分析，以检查UACRMACE和总死亡率风险之间的关联。进一步评估了UACR的额外预测价值。 类似的方法用于分析 这 UACR和MACE与正常范围内总死亡率在8.83年的中位随访期间，1808名（17.78%）受试者经历了MACE，总死亡率为1934名（19.01%）。在调整了传统的心血管危险因素后，多变量分析揭示了UACR与MACE和总死亡率风险之间的显着关联。将UACR纳入常规风险模型提高了对MACE和总死亡率的预测效果。An elevated UACR is associated with a higher risk of MACEs and total mortality in patients with T2DM, even when it falls below the normal range. UACR 改善了 2 型糖尿病患者的 MACE 和总死亡风险预测。