title: ÐÐ¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° DpsI Ð² Ñ Ð¾Ð´Ðµ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸ ÐºÐ»ÐµÑÐ¾Ðº Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð¾Ð¼ phiKZ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Localization of DpsI bacterial protein during cell infections by phiKZ bacteriophage
Creators: name: ÐÐ¸ÑÐ¸Ð¿Ð¾ÑÐµÐ½ÐºÐ¾, ÐÐ½Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³
phiKZ
Pseudomonas aeruginosa
Ð¸Ð½ÑÐµÐºÑÐ¸Ñ
Ð¿ÑÐµÐ²Ð´Ð¾-ÑÐ´ÑÐ¾
DpsI
ÑÐ²ÑÐ·ÑÐ²Ð°Ð½Ð¸Ðµ ÐÐÐ
ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð°Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ
bacteriophage
infection
pseudo-nucleus
DNA binding
fluorescent microscopy

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«ÐÐ¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° DpsI Ð² ÑÐ¾Ð´Ðµ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸ ÐºÐ»ÐµÑÐ¾Ðº Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð¾Ð¼ phiKZÂ» ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð½Ð°Ð±Ð»ÑÐ´ÐµÐ½Ð¸Ñ Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð² Ð¸Ð½ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð¾Ð¼ phiKZ ÐºÐ»ÐµÑÐºÐ°Ñ Ð±ÐµÐ»ÐºÐ° DpsI, ÐºÐ¾ÑÐ¾ÑÑÐ¹ Ð¿ÑÐµÐ´Ð¿Ð¾Ð»Ð¾Ð¶Ð¸ÑÐµÐ»ÑÐ½Ð¾ ÑÐ²ÑÐ·ÑÐ²Ð°ÐµÑ Ð¸ Ð·Ð°ÑÐ¸ÑÐ°ÐµÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÐ¹ Ð½ÑÐºÐ»ÐµÐ¾Ð¸Ð´ Ð¾Ñ ÐÐÐÐ°Ð· Ð²Ð¸ÑÑÑÐ° Ð²Ð¾ Ð²ÑÐµÐ¼Ñ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸. ÐÐ·Ð²ÐµÑÑÐ½Ð¾, ÑÑÐ¾ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³ phiKZ Ð¾Ð±ÑÐ°Ð·ÑÐµÑ Ð² ÐºÐ»ÐµÑÐºÐµ Ð¿ÑÐµÐ²Ð´Ð¾-ÑÐ´ÑÐ¾, ÐÐÐ ÑÐ¾Ð·ÑÐ¸Ð½Ð° Ð¿ÑÐ¸ÑÑÑÑÑÐ²ÑÐµÑ Ð² ÐºÐ»ÐµÑÐºÐµ Ð´Ð¾ Ð¿Ð¾ÑÐ»ÐµÐ´Ð½ÐµÐ¹ ÑÑÐ°Ð´Ð¸Ð¸ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸. ÐÑÐµÐ´Ð¿Ð¾Ð»Ð¾Ð¶Ð¸Ð»Ð¸, ÑÑÐ¾ ÐÐÐ Ð±Ð°ÐºÑÐµÑÐ¸Ð¸ Ð·Ð°ÑÐ¸ÑÐµÐ½Ð° Ð¾Ñ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ ÐÐÐÐ°Ð·, ÑÐ°Ðº ÐºÐ°Ðº Ð¾Ð½Ð° ÑÐ²ÑÐ·Ð°Ð½Ð° Ñ ÑÐµÑÐ¾ÑÐ¾Ð´ÐµÑÐ¶Ð°ÑÐ¸Ð¼Ð¸ ÐÐÐ-ÑÐ²ÑÐ·ÑÐ²Ð°ÑÑÐ¸Ð¼Ð¸ Ð±ÐµÐ»ÐºÐ°Ð¼Ð¸. ÐÐ´Ð½Ð¸Ð¼ Ð¸Ð· ÑÐ°ÐºÐ¸Ñ Ð±ÐµÐ»ÐºÐ¾Ð² ÑÐ²Ð»ÑÐµÑÑÑ Ð±ÐµÐ»Ð¾Ðº Ð¸Ð· Ð³Ð¾Ð»Ð¾Ð´Ð°ÑÑÐ¸Ñ ÐºÐ»ÐµÑÐ¾Ðº (DpsI), ÑÑÐ°Ð½ÑÐ»ÑÑÐ¸Ñ ÐºÐ¾ÑÐ¾ÑÐ¾Ð³Ð¾ Ð½Ð°ÑÐ¸Ð½Ð°ÐµÑÑÑ Ð² ÑÑÑÐµÑÑÐ¾Ð²ÑÑ ÑÑÐ»Ð¾Ð²Ð¸ÑÑ, ÑÐ²ÑÐ·Ð°Ð½Ð½ÑÑ Ñ Ð¸Ð½ÑÐµÐºÑÐ¸ÐµÐ¹. Ð¡ ÑÐµÐ»ÑÑ Ð¿ÑÐ¾Ð²ÐµÑÐºÐ¸ Ð´Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð¿ÑÐµÐ´Ð¿Ð¾Ð»Ð¾Ð¶ÐµÐ½Ð¸Ñ Ð¿ÐµÑÐµÐ´Ð¾ Ð¼Ð½Ð¾Ð¹ Ð±ÑÐ»Ð¸ Ð¿Ð¾ÑÑÐ°Ð²Ð»ÐµÐ½Ñ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð·Ð°Ð´Ð°ÑÐ¸: 1) Ð½Ð°ÑÐ°Ð±Ð¾ÑÐºÐ° ÐÐÐ-ÑÑÐ°Ð³Ð¼ÐµÐ½ÑÐ°, ÑÐ¾Ð´ÐµÑÐ¶Ð°ÑÐµÐ³Ð¾ Ð³ÐµÐ½ Ð±ÐµÐ»ÐºÐ° DpsI; 2) ÐºÐ»Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½Ð¾Ð³Ð¾ ÑÑÐ°Ð³Ð¼ÐµÐ½ÑÐ°, ÑÐ»Ð¸ÑÐ¾Ð³Ð¾ Ñ ÐºÐ¾Ð´Ð¸ÑÑÑÑÐµÐ¹ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÑÑ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ°, Ð² ÑÐ°ÑÑÐ»-Ð²ÐµÐºÑÐ¾Ñ; 3) ÑÑÐ°Ð½ÑÑÐ¾ÑÐ¼Ð°ÑÐ¸Ñ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½Ð¾Ð¹ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸ÐµÐ¹ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð¸ Ð¿Ð¾Ð´Ð±Ð¾Ñ ÑÑÐ»Ð¾Ð²Ð¸Ð¹ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸; 4) Ð¸Ð½ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÐºÐ»ÐµÑÐ¾Ðº Ð¸ Ð½Ð°Ð±Ð»ÑÐ´ÐµÐ½Ð¸Ðµ Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð±ÐµÐ»ÐºÐ° Ð² ÑÐ¾Ð´Ðµ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸. Ð Ð°Ð±Ð¾ÑÐ° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ ÐÐ°ÑÑÐ½Ð¾-Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÑÐºÐ¾Ð³Ð¾ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ° Â«ÐÐ°Ð½Ð¾Ð±Ð¸Ð¾ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¸Â» (ÐÐÐ Â«ÐÐ°Ð½Ð¾ÐÐ¸Ð¾Â») Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð±ÑÐ»Ð° Ð¿Ð¾Ð»ÑÑÐµÐ½Ð° ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ñ, ÐºÐ¾ÑÐ¾ÑÐ¾Ð¹ ÑÑÐ°Ð½ÑÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð»Ð¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÐµ ÐºÐ»ÐµÑÐºÐ¸ Ð¸ Ð½Ð°Ð±Ð»ÑÐ´Ð°Ð»Ð¸ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸. ÐÑÑÐ¾Ð´Ñ Ð¸Ð· ÐºÐ°ÑÐµÑÑÐ²ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð¸Ð·Ð¾Ð±ÑÐ°Ð¶ÐµÐ½Ð¸Ð¹ Ð¼Ð¾Ð¶Ð½Ð¾ ÑÐ´ÐµÐ»Ð°ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð²ÑÐ²Ð¾Ð´Ñ: Ð±ÐµÐ»Ð¾Ðº Ð½Ðµ Ð¿ÑÐ¾Ð½Ð¸ÐºÐ°ÐµÑ Ð² Ð¿ÑÐµÐ²Ð´Ð¾-ÑÐ´ÑÐ¾ Ð¸ ÑÐ°Ð²Ð½Ð¾Ð¼ÐµÑÐ½Ð¾ ÑÐ°ÑÐ¿ÑÐµÐ´ÐµÐ»ÑÐµÑÑÑ Ð² ÑÐ¸ÑÐ¾Ð¿Ð»Ð°Ð·Ð¼Ðµ Ð²Ð½Ðµ Ð¿ÑÐµÐ²Ð´Ð¾ÑÐ´ÑÐ°. ÐÐµÐ»Ð¾Ðº ÑÐºÐ»Ð¾Ð½ÐµÐ½ Ðº Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ñ Ð°Ð³ÑÐµÐ³Ð°ÑÐ¾Ð², Ð´Ð»Ñ Ð½Ð°Ð±Ð»ÑÐ´ÐµÐ½Ð¸Ñ ÐµÐ³Ð¾ Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ ÑÑÐµÐ±ÑÑÑÑÑ Ð¿ÑÐ°Ð²Ð¸Ð»ÑÐ½Ð¾ Ð¿Ð¾Ð´Ð¾Ð±ÑÐ°Ð½Ð½ÑÐµ ÑÑÐ»Ð¾Ð²Ð¸Ñ. ÐÐ° Ð´Ð°Ð½Ð½ÑÐ¹ Ð¼Ð¾Ð¼ÐµÐ½Ñ Ð¾ÑÑÐ°ÐµÑÑÑ Ð¾ÑÐºÑÑÑÑÐ¼ Ð²Ð¾Ð¿ÑÐ¾Ñ, ÑÐ²ÑÐ·ÑÐ²Ð°ÐµÑ Ð»Ð¸ Ð±ÐµÐ»Ð¾Ðº ÐÐÐ Ð¸Ð»Ð¸ Ð ÐÐ, Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼Ñ Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐ¸Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ.
description: Topic of graduation qualification work: Â«Localization of DNA-binding protein from starved cells (DpsI) Pseudomonas aeruginosa during infection with bacteriophage phiKZÂ» This work is intended to observe localization in bacteriophage-infected cells of the DpsI protein, which supposedly binds and protects the bacterial nucleoid from the DNAse of the phiKZ virus during infection. PhiKZ bacteriophage is known to form a pseudo-nucleus in a cell, with host DNA present in the cell until the final stage of infection. It has been suggested that bacterial DNA is protected from the action of DNA, as it is associated with sulfur-containing DNA-binding proteins. One such protein is a protein from starving cells (DpsI), whose translation begins in stressful conditions associated with infection. In order to verify this assumption, I have been given the following tasks: 1) amplification of a DNA fragment containing the gene DpsI; 2) cloning of the resulting fragment, merged with the fluorescent protein coding sequence, into a shuttle vector; 3) transformation of bacterial cell design and selection of expression observation condition; 4) cell infection and protein localization observation by fluorescent microscopy. The work was carried out on the basis of the research complex Â«NanobiotechnologiesÂ» (NIK Â«NanoBioÂ»). The result was a construction that was transformed by bacterial cells and observed expression by fluorescent microscopy. From a qualitative analysis of the images obtained, the following conclusions can be drawn: the protein does not penetrate the pseudo-nucleus and is distributed evenly in the cytoplasm outside the pseudo-nucleus. The protein is prone to the formation of aggregates, the observation of its localization requires well-chosen conditions. It is not clear whether the protein binds DNA or RNA, and further research is needed.