Pseudococcus luciae Caballero 2021, n. sp.
Creators: name: Caballero, Alejandro
Publisher: name: Zenodo
Year: 2021
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hemiptera
Pseudococcidae
Pseudococcus
Pseudococcus luciae

description: Pseudococcus luciae n. sp.
(Figs 7; 8)
urn:lsid:zoobank.org:act: FD789556-375E-48E7-97F1-332C3867273A
TYPE MATERIAL. — Holotype. Colombia • adult ♀ (2 specimens in the same slide, holotype at the right side indicated as “Holo” in additional label); Cauca, Caldono, Vereda Chindaco, Farm La Esmeralda; 02°48’36.68”N, 76°28’40.22”W; 1603 m a.s.l.; 28.XI.2015; J. Medina leg.; ex roots Coffea arabica (Rubiaceae) – coffee, manual; UNAB 4635.
Paratypes. 8 adult ♀ on 5 slides • 1 ♀ (marked as “Para” shares a slide with the holotype); same data as for holotype; UNAB 4635 • 1 ♀ (marked as “Para” shares a slide with an immature specimen); same data as for holotype; UNAB 4635 • 4 ♀ (on 2 slides); same data as for holotype; UNAB 4635 • 2 ♀ (on 1 slide); same data as for holotype; MNHN.
ETYMOLOGY. — The specific epithet “ luciae ” dedicated to Dr Lucia Calderón-Espinosa, Colombian herpetologist.
DIAGNOSIS. — Combination of only a few oral collar tubular ducts (<75 ducts), a few oral rim tubular ducts (<15 over entire body), and few multilocular disc pores (<12), a small circulus (<61 µm wide) and eyes not associated with either a sclerotized area nor with discoidal pores.
DESCRIPTION
Pre-mounting specimens
Appearance in situ: not observed. Specimens preserved in 75% ethanol with milky appearence; color unchanged in 10% potassium hydroxide.
Post-mounting specimens
Slide-mounted adult females (n = 6): body oval to round (Fig. 7; 8 A), length 1.9 ± 0.2 [2.2] (1.5-2.2) mm; wide 1.4 ± 0.3 [1.7] (0.9-1.7) mm.
Dorsum
Ostioles. Conspicuous, 20.4 ± 4.0 (13-30) µm wide, with membranous lips and 3-5 setae and scattered trilocular pores on each lip.
Anal ring. 76.1 ± 2.3 [77] (72-80) µm in transverse diameter, with 6 flagellate setae each 104.9 ± 8.9 (90-123) µm long; ring containing two rows of cells, external row with cells smaller than those in internal row, subquadrate and subrectangular, each cell with a spicule 2 or 3 µm long; internal row with 45.8 ± 4.0 (41- 53) irregular cells tending to be elongate and without spicules.
Cerarii. Numbering 17 pairs (Fig. 7A); cerarian pattern C 1 [2;4] (1-2;2-6), C 2 [2;4-5] (1-2;2-6), C 3 [2;2-4] (2;1-5), C 4 [2;2] (2;1-3), C 5 [2;1-3] (1-2;1-3), C 6 [2;0-1] (2;-4), C 7 [2;1] (2;1-3), C 8 [2-3;1-2] (1-3;0-2), C 9 [2;2] (2;0-3), C 10 [2;0-1] (1-3;0-1), C 11 [2;1-2] (2;1-3), C 12 [2;2-3] (1-2;0-5), C 13 [2;1] (1-2;1-4), C 14 [2-3;1-3] (1-3;1-3), C 15 [2;0-2] (1-2;0-3), C 16 [2-3;1-2] (2-4;0-3), C 17 [2;1-2] (1-4;0-3), all cerarii situated on membranous plates.
Body setae. Flagellate, 19.1 ± 6.5 (9-45) µm long, longest setae found in mesial and submarginal regions, shortest setae evenly distributed.
Trilocular pores. Evenly distributed over entire surface (Fig.7B), each pore 3-4 µm in diameter.
Multilocular disc pores. Absent.
Oral rim tubular ducts. All same size, each 8.6 ± 0.6 (8-10) µm in diameter and associated with 1 or 2 discoidal pores and 1 seta (Figs 7C; 8B), numbering 2.4 ± 1.5 [3] (0-5), distribution highly variable but never situated on head, thorax at the level of C 11 or on SabdIII and posterior segments.
Oral collar tubular ducts. Absent.
Discoidal pores. Of two types: type I with a single loculus, present in two sizes: I) small, each 2-3 µm in diameter, smaller than a trilocular pore (Fig. 7D), distributed from head to SabdVI; and II) larger, each 3-5 µm in diameter, similar size to, or larger than a trilocular pore (Fig. 7E), distributed on SabdVII and SabdVIII + IX, with border stout and sclerotized; type II discoidal pores bilocular, each 4-5 µm in diameter (Figs. 7F; 8C).
Spicule. Present from metathorax and posterior segments.
Venter
Antennae. Each 393.8 ± 13.5 (367-419) µm long (Fig. 7G; 8D), with eight segments, segment lengths and chaetotaxy as follows: SantI 61.9 ± 4.3 (55-71) µm long, with four flagellate setae; SantII 63.6 ± 3.2 (58-69) µm long, with five to eight flagellate setae and one placoid sensilla; SantIII 44.9 ± 3.3 (39- 51) µm long, with five to seven flagellate setae; SantIV 30.3 ± 2.8 (23-34) µm long, with three to five flagellate setae; SantV 36.4 ± 3.7 (31-44) µm long, with six to nine flagellate setae; SantVI 31.3 ± 2.4 (28-35) µm long, with five flagellate setae; SantVII 39.0 ± 2.1 (33-43) µm long, with five flagellate setae and one fleshy seta; SantVIII 86.4 ± 2.8 (79-91) µm long, with 18 flagellate setae and four fleshy setae; ratios SantI / SantII 1.0 ± 0.1 (0.8-1.2), SantI / SantIV 2.1 ± 0.2 (1.8-2.6), SantI / SantVI 2.0 ± 0.2 (1.7-2.3), and SantVIII / SantIV 2.9 ± 0.3 (2.5-3.7).
Eyespots. Each 39.1 ± 2.8 (33-43) µm in diameter, not associated with discoidal pores (Fig. 8).
Labium. 120.4 ± 4.6 (115-129) µm long and 114.7 ± 6.6 (101-121) µm wide, with three segments: basal segment with 3 pairs of setae; middle segment with one pair of setae; and apical segment with seven to nine pairs of setae.
Fore leg. 566.6 ± 10.8 (546-582) µm long; trochanter + femur 262.1 ± 6.3 (248-275) µm long, femur 198.0 ± 5.1 (188-205) long and 86.8 ± 4.0 (76-94) µm wide; tibia + tarsus 268.5 ± 6.6 (259-282) µm long, tibia 172.3 ± 5.2 (163-181) long and 43.9 ± 2.0 (39-47) µm wide; claw 36.1 ± 1.5 (32-39) µm long; ratios: lengths of trochanter + femur/tibia + tarsus 1.0; trochanter + femur length/femur width 3.0 ± 0.2 (2.8-3.4); tibia + tarsus length/tibia width 6.1 ± 0.3 (5.5-6.8); chaetotaxy: coxa with 10 flagellate setae, trochanter with seven setae and four placoid sensilla, femur with 17-22 setae, tibia with 18-20 flagellate setae plus two spines on internal margin of distal region; tarsus with 11-13 setae and a pair of slender and capitate tarsal digitules of two sizes, the larger one 40.3 ± 2.3 (35-46) µm long, the shorter one 30.0 ± 4.2 (23-38) µm long; claw without a denticle, with two ungual digitules capitate, slightly thicker than tarsal digitule, each 27.9 ± 1.8 (24-30) µm long.
Mid leg. 581.4 ± 11.4 (562-601) µm long; trochanter + femur 269.3 ± 6.0 (258-282) µm long, femur 205.1 ± 4.8 (196-214) long and 89.4 ± 1.9 (87-92) µm wide; tibia + tarsus 275.3 ± 7.8 (257-288) µm long, tibia 178.2 ± 6.5 (163-191) long and 45.8 ± 2.2 (41-49) µm wide; claw 36.8 ± 1.8 (32-39) µm long; ratios: lengths of trochanter + femur/tibia + tarsus 1.0, trochanter + femur length/femur width 3.0 ± 0.1 (2.8- 3.2), tibia + tarsus length/tibia width 6.0 ± 0.4 (5.2-6.9); chaetotaxy: coxa with 11 flagellate setae, trochanter with seven, femur with 16-20, tibia with 18-28 flagellate setae plus two spines (as fore tibia), tarsus with 10-13 setae and a pair of unequal tarsal digitules similar those on fore tarsus, the larger one 38.2 ± 1.9 (36-42) µm long, the shorter one 32.4 ± 2.6 (28-36) µm long; claw without a denticle, ungual digitules similar those on fore claw, each digitule 29.4 ± 2.5 (21-32) µm long.
Hind leg. 655.1 ± 11.6 (637-678) µm long; trochanter + femur 295.9 ± 5.8 (282-306) µm long; femur 228.8 ± 4.3 (219-234) µm long and 94.6 ± 3.0 (88-101) µm wide; tibia + tarsus 322.7 ± 7.3 (312-334) µm long; tibia 219.0 ± 6.0 (210-228) µm long and 44.5 ± 3.2 (37-51) µm wide; claw 37.2 ± 2.7 (30-41) µm long; ratios: lengths of trochanter + femur/tibia + tarsus 0.9-1.0; trochanter + femur length/ femur width 3.1 ± 0.1 (2.9-3.4), tibia + tarsus length/tibia width 7.3 ± 0.6 (6.4-8.7), lengths of tibia/tarsus 2.1 ± 0.1 (2.0-2.2).Translucent pores on hind leg distributed as follows: absent from coxa, trochanter and tarsus, femur with 90 ± 12 (69-112) pores, tibia with 58 ± 12 (34-74) pores (Fig. 7H); chaetotaxy: coxa with 11 flagellate setae, trochanter with seven, femur with 14-17, tibia with 20-26 flagellate setae plus two spines (similar those on fore tibia), tarsus with 8-10 flagellate setae; tarsal digitules unequal, similar those on fore tarsus, the larger one 38.6 ± 3.4 (31-45) µm long, the shorter one 39.9 ± 4.6 (21-37) µm long (Fig. 8F); claw without a denticle, ungual digitules similar to those on other legs, each 29.3 ± 2.8 (21-32) µm long.
Circulus. Situated in posterior part of SabdIII, 48.9 ± 6.0 [61] (41-61) µm in transverse diameter (Fig. 8G).
Anal lobes. Prominent, not sclerotized, each with apical flagellate seta 127.3 ± 11.0 (104-144) µm long, 2-7 auxiliary flagellate setae and 1-2 discoidal pores, each 4-5 µm in diameter (Fig. 8H).
Body setae. Flagellate and of variable length: 50.2 ± 21.4 (21- 88) µm long on head, 31.9 ± 12.9 (14-65) µm on thorax, and 33.0 ± 14.0 (11-80) µm long on abdomen, the longest setae distributed in mesial region on all segments. Intersegmental areas lacking setae.
Obanal setae. Each 60.4 ± 5.1 (51-69) µm long.
Cisanal setae. Each 49.3± 5.7 (40-57) µm long.
Trilocular pores. Each 3-4 µm in diameter (Fig. 7I), evenly distributed.
Multilocular disc pores. Each 8.4 ± 0.6 (7-10) µm in diameter (Fig. 7J; 8I), numbering 5.8 ± 2.7 [3] (2-11), distributed as follows: SabdV [0] (0-1), SabdVI [0] (0-1), SabdVII [0] (0-5) and SabdVIII + IX [3] (1-6).
Discoidal pores. Of two types: I) one type with a single loculus each 4-5 µm in diameter, evenly distributed (Fig. 7K); II) second type bilocular, each pore 5-6 µm in diameter, scarce (Fig. 7L).
Oral rim tubular ducts. All same size, each 8.8 ± 0.9 (7-10) µm in diameter, length about 11.3± 1.2 (10-12), usually not associated with discoidal pores or setae; numbering 1.9 ± 2.3 [7] (0-7) (Fig. 7M), distribution highly variable but absent from SabdIV and posterior segments.
Oral collar tubular ducts. All same size, each 7.4 ± 0.8 (6- 10) µm long and 4.1 ± 0.4 (3-6) µm in diameter (Fig. 7N, Fig. 8J), numbering 63.1 ± 8.5 [70] (53-71), forming lateral clusters and transverse rows in mid-region as follows: SabdIII [2] (0-6), SabdIV [22] (11-26), SabdV [29] (20-33), SabdVI [30] (21-33), and SabdVII [17] (12-18) ducts.
Spicules. Present on mesothorax and posterior body segments.
REMARKS
Pseudococcus luciae n. sp. is similar to Pseudococcus dendrobiorum Williams, 1985 and Pseudococcus lycopodii Beardsley, 1959 because all three species have only a few multilocular disc pores, restricted to the last three abdominal segments (<12 in P. luciae n. sp., <13 in P. dendrobiorum and <15 in P. lycopodii); only a few dorsal oral rim tubular ducts on the dorsum (<5 in P. luciae n. sp., 0 in P. dendrobiorum and 4-12 in P. lycopodii) and venter (<7 in P. luciae n. sp., <3 in P. dendrobiorum and 4-12 in P. lycopodii, absent from SabdIV and posterior segments). Pseudococcus luciae n. sp. differs from P.dendrobiorum (characters in parenthesis) by having a shorter labium, less than 130 µm long (150-160 µm long), a smaller circulus, less than 60 µm of transverse diameter (100-130 µm in transverse diameter), a longer hind tibia + tarsus, around 310-340 µm long (160-200 µm long), and hind tibia + tarsus length greater than hind trochanter +femur length (hind tibia + tarsus much shorter than hind trochanter + femur). Pseudococcus luciae n. sp. differs from P. lycopodii (characters in parenthesis) by having 17 cerarii (14-16 cerarii), more than 50 collar tubular ducts (10-20) and a smaller circulus (80 µm in transverse diameter) [character data on P. dendrobiorum taken from Williams (1985) and on P. lycopodii from Beardsley (1959)].
The taxonomic character “transverse diameter of circulus” is considered to be useful for separating species groups (Gimpel & Miller 1996). Pseudococcus luciae n. sp. belongs to the “small-circulus” (transverse diameter less than 80 µm) group, which includes P. apomicrocirculus Gimpel & Miller, 1996, P. dolichomelos Gimpel & Miller, 1996, P. debilis Granara de Willink, 2018, P. dysmicus Gimpel & Miller, 1996, P. eriocerei Williams, 1973, P. microcirculus McKenzie, P. neomicrocirculus Gimpel & Miller, 1996, P. sorghiellus (Forbes, 1885), and P. spanocera. Among these species, P.luciae n. sp. is closest to P.neomicrocirculus and P.spanocera. In addition to having a small circulus, these species have only a few oral rim tubular ducts. Morphological differences between them include absence of discoidal pores associated with eyespot (P. neomicrocirculus and P. spanocera have discoidal pores associated with eyespots), translucent pores restricted to femur and tibia (present in all segments of the hind leg in P. spanocera; restricted to the hind tibia in P. neomicrocirculus), and less than five multilocular disc pores on each abdominal segment from SabdV and posterior segments (P. neomicrocirculus and P. spanocera have more than 10 multilocular disc pores on each abdominal segment) [character data of P. neomicrocirculus and P. spanocera taken from Gimpel & Miller (1996)].
description: Published as part of Caballero, Alejandro, 2021, Four new scale insect species (Hemiptera: Coccomorpha) associated with coffee roots in Colombia, South America, with identification keys for genera Newsteadia Green, 1902, Distichlicoccus Ferris, 1950, and Paraputo Laing, 1929, pp. 341-363 in Zoosystema 43 (18) on pages 356-361, DOI: 10.5252/zoosystema2021v43a18, http://zenodo.org/record/5037821