Additional file 1 of MCP mediated active targeting calcium phosphate hybrid nanoparticles for the treatment of orthotopic drug-resistant colon cancer
Creators: name: Bai, Shaobo
name: Sun, Yang
name: Cheng, Ying
name: Ye, Weiliang
name: Jiang, Chenchao
name: Liu, Miao
name: Ji, Qifeng
name: Zhang, Bangle
name: Mei, Qibing
name: Liu, Daozhou
name: Zhou, Siyuan
Publisher: name: figshare
Year: 2021
Subjects: subject: Biochemistry
subject: Space Science
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Molecular Biology
subject: Pharmacology
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Cancer
subject: Hematology
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Virology

description: Additional file 1: Figure S1 Immunofluorescence staining of Gal-3 in the colon tissue of normal nude mouse. Blue: nucleus; red: Gal-3 protein. Figure S2. XPS analysis of phosphorus (A), calcium (B), carbon (C), and oxygen (D) in PSVII@MCP-CaP. Figure S3. Thermogravimetric analysis of calcium phosphate powder (A), MCP (B), PSVII@CM-β-CD inclusion compound (C), @MCP-CaP (D), and PSVII@MCP-CaP (E). Figure S4. The cellular uptake mechanism of PSVII@MCP-CaP by HCT116/L cell. A The effect of uptake inhibitor on the uptake of PSVII@MCP-CaP by HCT116/L cell observed via LSCM. B Semi-quantitative analysis results of PSVII@MCP-CaP uptake by HCT116/L cell. All data are expressed as mean ± SD (n = 3). **p &lt; 0.01 vs control. Figure S5. The inhibitory effect of PSVII@MCP-CaP on proliferation of HT-29 cell and SW620 cell in vitro. A The inhibitory effect of PSVII@MCP-CaP on the proliferation of HT-29 cell. B The inhibitory effect of PSVII@MCP-CaP on the proliferation of SW620 cell. C Live/dead cell staining of HT-29 cell. D Semi-quantitative statistical results of the ratio between dead HT-29 cell and live HT-29 cell. E Live/dead cell staining of SW620 cell. F Semi-quantitative statistical results of the ratio between dead SW620 cell and live SW620 cell. All data are expressed as mean ± SD (n = 5). **p &lt; 0.01 vs L-OHP. Figure S6. The statistical results of cell clone formation and growth of cell spheres. A Semi-quantitative statistical results of HCT116 cell clone formation. B Semi-quantitative statistical results of HCT116/L cell clone formation. C The effect of PSVII@MCP-CaP on the growth of HCT116 cell spheres. D The effect of PSVII@MCP-CaP on the growth of HCT116/L cell spheres. All data are expressed as mean ± SD (n = 3). **p &lt; 0.01 vs control; ##p &lt; 0.01 vs PBS. Figure S7. Effect of PSVII@MCP-CaP on the migration and invasion of HT-29 cell and SW620 cell. A Typical images of HT-29 cell migrated to transwell recipient chamber (× 200). B Semi-quantitative statistical results of HT-29 cell migration. C Typical images of HT-29 cell invaded to transwell recipient chamber (× 200). D Semi-quantitative statistical results of HT-29 cell invasion. E Typical images of SW620 cell migrated to transwell recipient chamber (× 200). F Semi-quantitative statistical results of SW620 cell migration. G Typical images of SW620 cell invaded to transwell recipient chamber (× 200). H Semi-quantitative statistical results of SW620 cell invasion. I Effect of PSVII@MCP-CaP on invasion-related proteins expression in HT-29 cell. J Semi-quantitative statistical results of invasion-related proteins expression in HT-29 cell. K Effect of PSVII@MCP-CaP on invasion-related proteins expression in SW620 cell. L Semi-quantitative statistical results of invasion-related proteins expression in SW620 cell. All data are expressed as means ± SD (n = 3). *p &lt; 0.05, **p &lt; 0.01 vs PSVII (0.5 µmol/L); #p &lt; 0.05, ##p &lt; 0.01 vs PSVII (1.0 µmol/L)