title: Ð¤ÑÐ½ÐºÑÐ¸Ð¾Ð½Ð°Ð»Ð¸Ð·Ð°ÑÐ¸Ñ ÑÑÃ«ÐºÐ¾Ð» Ñ Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸ÑÐ°Ð¼Ð¸ ÑÐµÑÐµÐ±ÑÐ° Ð±ÐµÐ»ÐºÐ¾Ð¼ TMBP Ð´Ð»Ñ Ð±Ð¸Ð¾ÑÐµÐ½ÑÐ¾ÑÐ½ÑÑ Ð¿ÑÐ¸Ð»Ð¾Ð¶ÐµÐ½Ð¸Ð¹
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: The functionalization of glass substrates with a silver nanoparticles by protein for biosensor applications
Creators: name: ÐÐµÐ»ÑÑÐ¾Ð²Ð°, ÐÐ»ÑÐ³Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Ð¡ÑÐµÐºÐ»Ð¾
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ÐÐ¸Ð¾ÑÐµÐ½ÑÐ¾ÑÑ
ÑÐµÑÐµÐ±ÑÑÐ½ÑÐµ Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸ÑÑ
Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð¾Ð²Ð°Ð½Ð½ÑÐ¹ Ð¿Ð»Ð°Ð·Ð¼Ð¾Ð½Ð½ÑÐ¹ ÑÐµÐ·Ð¾Ð½Ð°Ð½Ñ
ÑÑÐµÐ³Ð°Ð»Ð¾Ð·Ð°(Ð¼Ð°Ð»ÑÑÐ¾Ð·Ð°)-ÑÐ²ÑÐ·ÑÐ²Ð°ÑÑÐ¸Ð¹ Ð±ÐµÐ»Ð¾Ðº
Ð³Ð»ÑÐºÐ¾Ð·Ð°
silver nanoparticles
localized plasmon resonance
trehalose (maltose)-binding protein
glucose

description: Ð¦ÐµÐ»Ñ ÑÐ°Ð±Ð¾ÑÑ â ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð°Ð»Ð¸Ð·Ð°ÑÐ¸Ñ ÑÑÐµÐºÐ»ÑÐ½Ð½ÑÑ Ð¿Ð¾Ð´Ð»Ð¾Ð¶ÐµÐº Ñ ÐÐ§ ÑÐµÑÐµÐ±ÑÐ° Ð±ÐµÐ»ÐºÐ¾Ð¼ Ð´Ð»Ñ Ð±Ð¸Ð¾ÑÐµÐ½ÑÐ¾ÑÐ½ÑÑ Ð¿ÑÐ¸Ð»Ð¾Ð¶ÐµÐ½Ð¸Ð¹. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ» Ð¿ÑÐ¾Ð²ÐµÐ´ÑÐ½ Ð°Ð½Ð°Ð»Ð¸Ð· Ð»Ð¸ÑÐµÑÐ°ÑÑÑÑ, Ð¿Ð¾ÑÐ²ÑÑÑÐ½Ð½Ð¾Ð¹ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ°Ð¼ Ð±Ð¸Ð¾ÑÐµÐ½ÑÐ¾ÑÐ¾Ð² Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸Ñ Ð¼ÐµÑÐ°Ð»Ð»Ð¾Ð², ÑÐ°ÑÑÐ¼Ð¾ÑÑÐµÐ½Ñ ÑÐ¿Ð¾ÑÐ¾Ð±Ñ Ð¸Ð¼Ð¼Ð¾Ð±Ð¸Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð±ÐµÐ»ÐºÐ¾Ð², Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð° ÑÑÑÐ°Ð½Ð¾Ð²ÐºÐ° Ð´Ð»Ñ ÑÐ¸Ð½ÑÐµÐ·Ð° Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸Ñ ÑÐµÑÐµÐ±ÑÐ°, Ð¾ÑÐ²Ð¾ÐµÐ½Ð° Ð¼ÐµÑÐ¾Ð´Ð¸ÐºÐ° Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ Ð±ÐµÐ»ÐºÐ° Ð¸ Ð¿ÑÐ¾Ð²ÐµÑÐºÐ¸ ÐµÐ³Ð¾ Ð¾ÑÐ¸ÑÑÐºÐ¸, Ð¾Ð¿ÑÐ¸Ð¼Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°Ð½Ð° Ð¿ÑÐ¾ÑÐµÐ´ÑÑÐ° Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ½Ð¾Ð³Ð¾ Ð¿Ð¾ÐºÑÑÑÐ¸Ñ, Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ñ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÑ Ñ Ð¼Ð¾Ð´ÐµÐ»ÑÐ½ÑÐ¼Ð¸ ÑÐ°ÑÑÐ²Ð¾ÑÐ°Ð¼Ð¸ Ð¸ ÑÐ°ÑÑÐ²Ð¾ÑÐ°Ð¼Ð¸ Ð³Ð»ÑÐºÐ¾Ð·Ñ. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð±ÑÐ»Ð° Ð¿Ð¾Ð»ÑÑÐµÐ½Ð° Ð²ÑÑÐ¾ÐºÐ¾ÑÑÐ²ÑÑÐ²Ð¸ÑÐµÐ»ÑÐ½Ð°Ñ ÑÐµÐ½ÑÐ¾ÑÐ½Ð°Ñ ÑÐ¸ÑÑÐµÐ¼Ð°, ÑÐµÐ°Ð³Ð¸ÑÑÑÑÐ°Ñ Ð½Ð° Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ Ð³Ð»ÑÐºÐ¾Ð·Ñ Ð² ÑÐ°ÑÑÐ²Ð¾ÑÐµ, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð° ÑÐ¿ÐµÐºÑÑÐ°Ð»ÑÐ½Ð°Ñ ÑÑÐ²ÑÑÐ²Ð¸ÑÐµÐ»ÑÐ½Ð¾ÑÑÑ, Ð²ÐµÐ»Ð¸ÑÐ¸Ð½Ð° ÐºÐ¾ÑÐ¾ÑÐ¾Ð¹ Ð² ÑÐ°Ð·Ñ Ð¿ÑÐµÐ²Ð¾ÑÑÐ¾Ð´Ð¸Ñ Ð¸Ð¼ÐµÑÑÐ¸ÐµÑÑ Ð°Ð½Ð°Ð»Ð¾Ð³Ð¸. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ ÑÐµÐ³Ð¾ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð½Ð°Ñ ÑÐ¸ÑÑÐµÐ¼Ð° ÑÐ²Ð»ÑÐµÑÑÑ Ð¿ÐµÑÑÐ¿ÐµÐºÑÐ¸Ð²Ð½Ð¾Ð¹ Ð´Ð»Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ñ Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð³Ð»ÑÐºÐ¾Ð¼ÐµÑÑÐ°. ÐÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ñ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ð½ÑÐµ ÑÑÐµÐ´ÑÑÐ²Ð°: Image J Ð¸ Origin Lab Ð´Ð»Ñ ÑÐµÑÐµÐ½Ð¸Ñ Ð½Ð°ÑÑÐ½ÑÑ Ð·Ð°Ð´Ð°Ñ: Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ ÑÐ°Ð·Ð¼ÐµÑÐ¾Ð² Ð¸ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð° ÑÐ¸Ð½ÑÐµÐ·Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸Ñ Ð¸ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÐµÐ½Ð¸Ðµ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð² Ð² Ð²Ð¸Ð´Ðµ Ð³ÑÐ°ÑÐ¸ÐºÐ¾Ð².
description: The given work is devoted to the functionalization of glass substrates with a silver LF protein for biosensor applications. As a result of the study, the literature devoted to the development of biosensors using metal nanoparticles was analyzed, methods of protein immobilization were considered, a facility for the synthesis of silver nanoparticles was modified, a method for obtaining protein and checking its purification was mastered, the procedure for modifying the polymer coating was optimized, experiments with model solutions and glucose solutions were conducted. As a result, a highly sensitive sensor system was obtained that responds to changes in glucose concentration in solution, and spectral sensitivity was determined, the value of which is several times higher than the available analogues. As a result, the developed system is promising for use as a glucose meter. Software tools: ImageJ and Origin Lab were used to solve scientific problems: determining the size and quantity of synthesized nanoparticles and presenting experimental results in the form of graphs.