title: ÐÐ°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ñ Ð½Ð° Ð¼Ð¸ÑÐµÐ½Ð¸ Ð´Ð»Ñ ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐ½Ð¾Ð¹ ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸ Ð°Ð´Ð´ÑÐºÑÐ¾Ð² Ð±ÐµÐ»ÐºÐ¾Ð² ÐºÑÐ¾Ð²Ð¸ Ñ Ð³Ð°Ð»Ð¾Ð³ÐµÐ½Ð¾ÑÐ¾Ð´ÐµÑÐ¶Ð°ÑÐ¸Ð¼Ð¸ ÐºÑÐµÐ½Ð¾Ð±Ð¸Ð¾ÑÐ¸ÐºÐ°Ð¼Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Lab-on-a-plate for the specific extraction of adducts of blood proteins with halogenated xenobiotics
Creators: name: ÐÐ°Ð»Ð½Ð¸Ð½Ñ, Ð¯Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Ð¥ÑÐ¾Ð¼Ð°ÑÐ¾-Ð¼Ð°ÑÑ-ÑÐ¿ÐµÐºÑÑÐ¾Ð¼ÐµÑÑÐ¸Ñ
ÐÐ¸Ð¾ÑÐµÑ Ð½Ð¾Ð»Ð¾Ð³Ð¸Ñ
Ð°Ð»ÐºÐ¸Ð»Ð¸ÑÑÑÑÐ¸Ðµ Ð°Ð³ÐµÐ½ÑÑ
Ð±ÐµÐ»ÐºÐ¸ ÐºÑÐ¾Ð²Ð¸
Ð°Ð´Ð´ÑÐºÑÑ
Ð³Ð°Ð»Ð¾Ð³ÐµÐ½ÑÐ¾Ð´ÐµÑÐ¶Ð°ÑÐ¸Ðµ ÐºÑÐµÐ½Ð¾Ð±Ð¸Ð¾ÑÐ¸ÐºÐ¸
Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸ÑÑ Ð´Ð¸ÐºÐ»Ð¾ÑÐµÐ½Ð°ÐºÐ°
Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ñ Ð½Ð° Ð¼Ð¸ÑÐµÐ½Ð¸
alkyling agents
blood proteins
adducts
halogen-containing xenobiotics
diclofenac metabolites
lab-on-a-plate

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description: Theme of the master's work: Â«Lab-on-a-plate for the specific extraction of adducts of blood proteins with halogenated xenobioticsÂ». The work is devoted to the development of a method for the determination of halogen-containing adducts of blood proteins directly on a MALDI target. The Lab-on-plate approach speeds up and simplifies the sample preparation process, which makes it promising for solving proteomic and bioorganic problems. The process of formation of multimolecular structures of a metal-affinity sorbent based on lanthanum stearate monolayers is characterized and sorbents based on Cu, Ni, Co, Fe, In, Ga, Eu, and La stearates are obtained, optimal parameters are selected for obtaining sorbents and performing metal-affinity extraction on a target MALDI. It was shown that the resulting structures have a developed surface accessible to the analyte, and also exhibit affinity for chlorinecontaining peptides modified with chlorine-containing xenobiotics. Metal-affinity extraction of human globin adducts with two model chlorinecontaining alkylating agents N1-(4-chlorophenyl)-2-chloroacetamide (Cl-1) and N1-(2,4-dichlorophenyl)-2-chloroacetamide (Cl-2) was carried out using the method "Lab-on-plate". Based on the results of mass spectrometric analysis, a marker tryptic peptide LLGNVLVCVLAHHFGK of the human globin beta subunit was established, the structure of which includes C-112, which is sensitive to modification by alkylating agents. The approach was tested on the drug Diclofenac, which has a structural unit similar to the model agent Cl-2. The marker peptide modified with diclofenac UV/TiO2-PCO products was reliably identified after metal-affinity extraction directly on the MALDI target. The developed method "Lab-on-plate", characterized by sensitivity, high throughput and rapidity, can be used to detect active metabolites of drugs at the stage of preclinical trials, as well as for the retrospective diagnosis of xenobiotic poisoning.