title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ ÐºÐ¾Ð¿Ð¸Ð¹Ð½Ð¾ÑÑÐ¸ ÐºÐ¾Ð¼Ð¿Ð¾Ð½ÐµÐ½Ñ ÑÐ¸ÑÑÐµÐ¼Ñ ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Ð½Ð° Ð·Ð°ÑÐ¸ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð¾Ñ Ð²Ð¸ÑÑÑÐ½Ð¾Ð¹ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ° Ð¿Ð¾ Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð¸Ñ 03.04.02 - Ð¤Ð¸Ð·Ð¸ÐºÐ° ; 03.04.02_02 - ÐÐ¸Ð¾ÑÐ¸Ð·Ð¸ÐºÐ°
titleType: Subtitle
Creators: name: ÐÐ¸ÑÐ¸Ð»Ð»Ð¾Ð², ÐÐ»ÐµÐºÑÐ°Ð½Ð´Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2019
Subjects: ÑÐ¸ÑÑÐµÐ¼Ð° ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸
ÑÐ½Ð´Ð¾Ð½ÑÐºÐ»ÐµÐ°Ð·Ð° ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸
Ð¼ÐµÑÐ¸Ð»ÑÑÐ°Ð½ÑÑÐµÑÐ°Ð·Ð°
Esp1396I
Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³ Î»
Ð²Ð¸ÑÑÑÐ½Ð°Ñ Ð¸Ð½ÑÐµÐºÑÐ¸Ñ

description: Ð¡Ð¸ÑÑÐµÐ¼Ñ ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Ð¸Ð·Ð²ÐµÑÑÐ½Ñ ÐºÐ°Ðº Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ ÑÐ°ÑÐ¿ÑÐ¾ÑÑÑÐ°Ð½ÐµÐ½Ð½ÑÐµ ÑÐ¸ÑÑÐµÐ¼Ñ Ð·Ð°ÑÐ¸ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ Ð¾Ñ Ð²Ð¸ÑÑÑÐ¾Ð². ÐÐ´Ð½Ð°ÐºÐ¾ Ð² Ð½ÐµÐºÐ¾ÑÐ¾ÑÑÑ ÑÐ»ÑÑÐ°ÑÑ Ð·Ð°ÑÐ¸ÑÐ°, Ð¾Ð±ÐµÑÐ¿ÐµÑÐ¸Ð²Ð°ÐµÐ¼Ð°Ñ ÑÐ°ÐºÐ¸Ð¼Ð¸ ÑÐ¸ÑÑÐµÐ¼Ð°Ð¼Ð¸, Ð¼Ð¾Ð¶ÐµÑ Ð±ÑÑÑ Ð¿ÑÐµÐ¾Ð´Ð¾Ð»ÐµÐ½Ð° Ð²Ð¸ÑÑÑÐ°Ð¼Ð¸. ÐÐ°ÑÐ° ÑÐ°Ð±Ð¾ÑÐ° Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð° Ð½Ð° Ð¿Ð¾Ð½Ð¸Ð¼Ð°Ð½Ð¸Ðµ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð², ÐºÐ¾ÑÐ¾ÑÑÐµ Ð²Ð»Ð¸ÑÑÑ Ð½Ð° Ð¿ÑÐµÐ¾Ð´Ð¾Ð»ÐµÐ½Ð¸Ðµ Ð·Ð°ÑÐ¸ÑÑ Ð² ÐºÐ»ÐµÑÐºÐ°Ñ E. coli, Ð½ÐµÑÑÑÐ¸Ñ ÑÐ¸ÑÑÐµÐ¼Ñ ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ ÑÐ¸Ð¿Ð° II Esp1396I, ÑÐ°ÑÐ¿Ð¾Ð»Ð¾Ð¶ÐµÐ½Ð½ÑÑ Ð½Ð° Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð´Ðµ, Ð¸ Ð½Ð° Ð¾ÑÐµÐ½ÐºÑ ÑÐºÐ¾ÑÐ¾ÑÑÐ¸ Ð´ÐµÐ³ÑÐ°Ð´Ð°ÑÐ¸Ð¸ Ð²Ð¸ÑÑÑÐ½Ð¾Ð¹ ÐÐÐ Ð² Ð¾Ð´Ð½Ð¾Ð¹ Ð¸Ð½ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð¹ ÐºÐ»ÐµÑÐºÐµ, Ð½ÐµÑÑÑÐµÐ¹ ÑÐ¸ÑÑÐµÐ¼Ñ Esp1396I. ÐÐ»Ñ Ð¾ÑÐµÐ½ÐºÐ¸ ÑÐ¾Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ñ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð° ÑÐ½Ð´Ð¾Ð½ÑÐºÐ»ÐµÐ°Ð·Ñ ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸ Ð¸ Ð¼ÐµÑÐ¸Ð»ÑÑÐ°Ð½ÑÑÐµÑÐ°Ð·Ñ Ð² Ð¾ÑÐ´ÐµÐ»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ Ð¿ÑÐ¸Ð¼ÐµÐ½ÑÐ»ÑÑ Ð¼ÐµÑÐ¾Ð´ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸. ÐÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ ÑÑÐ¾Ð²ÐµÐ½Ñ Ð·Ð°ÑÐ¸ÑÑ ÐºÐ¾ÑÑÐµÐ»Ð¸ÑÑÐµÑ Ñ Ð°Ð±ÑÐ¾Ð»ÑÑÐ½ÑÐ¼ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð¾Ð¼ ÑÐµÑÐ¼ÐµÐ½ÑÐ¾Ð² Ð² ÐºÐ»ÐµÑÐºÐµ Ð¸ Ð¾ÑÐ½Ð¾ÑÐ¸ÑÐµÐ»ÑÐ½ÑÐ¼ ÑÐ¾Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸ÐµÐ¼ ÑÐ½Ð´Ð¾Ð½ÑÐºÐ»ÐµÐ°Ð·Ñ ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸ Ð¸ Ð¼ÐµÑÐ¸Ð»ÑÑÐ°Ð½ÑÑÐµÑÐ°Ð·Ñ Ð² Ð¾Ð´Ð¸Ð½Ð¾ÑÐ½ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ. ÐÑÐ¾Ð¼Ðµ ÑÐ¾Ð³Ð¾, Ð²Ð¿ÐµÑÐ²ÑÐµ Ð² ÑÐµÐ°Ð»ÑÐ½Ð¾Ð¼ Ð²ÑÐµÐ¼ÐµÐ½Ð¸ Ð±ÑÐ» Ð²Ð¸Ð·ÑÐ°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°Ð½ Ð¿ÑÐ¾ÑÐµÑÑ Ð´ÐµÐ³ÑÐ°Ð´Ð°ÑÐ¸Ð¸ ÐÐÐ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð° Ð² Ð¾Ð´Ð¸Ð½Ð¾ÑÐ½Ð¾Ð¹ ÐºÐ»ÐµÑÐºÐµ, Ð½ÐµÑÑÑÐµÐ¹ ÑÐ¸ÑÑÐµÐ¼Ñ ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸, Ð¸ Ð±ÑÐ»Ð° Ð¾ÑÐµÐ½ÐµÐ½Ð° ÑÐºÐ¾ÑÐ¾ÑÑÑ Ð´ÐµÐ³ÑÐ°Ð´Ð°ÑÐ¸Ð¸.
description: Restriction-modification systems are known as the most common protective systems of bacteria against viruses. Our work aims to understand factors that affect the overcoming of protection in E. coli cells carrying type II restriction-modification system Esp1396I located on a plasmid and to estimate the rate of bacteriophage DNA degradation in single infected cell carrying the Esp1396I system. It was shown that the level of protection correlated with the absolute level of enzymes and the relative ratio of endonuclease and methyltransferase in single cells. Also, for the first time the process of bacteriophage DNA degradation in a single cell carrying restriction-modification system was visualized in real time and the rate of degradation was estimated.