title: ÐÑÑÑÐ½ÐµÐ½Ð¸Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð²Ð½ÐµÑÐ½Ð¸Ñ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð² Ð½Ð° Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ¸Ð·Ð°ÑÐ¸Ñ Ð±ÐµÐ»ÐºÐ¾Ð² FtsZ Acholeplasma laidlawii Ð¸ Mycoplasma gallisepticum
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Elucidation of the influence of external factors on polymerization of FtsZ protein of Acholeplasma laidlawii and Mycoplasma gallisepticum
Creators: name: ÐÐ¾Ð»Ð¾ÑÐµÐµÐ²Ð°, ÐÐ°ÑÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: FtsZ
Acholeplasma laidlawii
Mycoplasma gallisepticum
Mollicutes
ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ðµ Ð´ÐµÐ»ÐµÐ½Ð¸Ðµ
cell division

description: Ð Ð°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ ÑÐ°ÑÐ°ÐºÑÐµÑÐ° Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ¸Ð·Ð°ÑÐ¸Ð¸ Ð±ÐµÐ»ÐºÐ¾Ð² FtsZ Acholeplasma laidlawii Ð¸ Mycoplasma gallisepticum Ð² ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ñ inÂ vitroÂ Ð² Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾ÑÑÐ¸ Ð¾Ñ Ð²Ð½ÐµÑÐ½Ð¸Ñ ÑÑÐ»Ð¾Ð²Ð¸Ð¹. ÐÑÐ¾ÑÐµÑÑ ÑÐ±Ð¾ÑÐºÐ¸ ÑÐ¸Ð»Ð°Ð¼ÐµÐ½ÑÐ¾Ð² FtsZ Ð² Ð¿ÑÐ¸ÑÑÑÑÑÐ²Ð¸Ð¸ ÐÐ¢Ð¤ ÑÐµÐ³Ð¸ÑÑÑÐ¸ÑÐ¾Ð²Ð°Ð»ÑÑ Ð¿ÑÐ¸ Ð¿Ð¾Ð¼Ð¾ÑÐ¸ Ð¼ÐµÑÐ¾Ð´Ð° ÑÑÐ°ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐ²ÐµÑÐ¾ÑÐ°ÑÑÐµÑÐ½Ð¸Ñ.ÐÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð²ÑÑÐ¾ÐºÐ¸Ð¹ ÑÑÐ¾Ð²ÐµÐ½Ñ pH, Ð° ÑÐ°ÐºÐ¶Ðµ Ð²ÑÑÐ¾ÐºÐ°Ñ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ñ Ð¸Ð¾Ð½Ð¾Ð² ÐºÐ°Ð»Ð¸Ñ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÑÑÑ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ¸Ð·Ð°ÑÐ¸Ñ Ð¾Ð±Ð¾Ð¸Ñ Ð±ÐµÐ»ÐºÐ¾Ð². ÐÐ¿ÑÐ¸Ð¼Ð°Ð»ÑÐ½Ð°Ñ Ð´Ð»Ñ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ¸Ð·Ð°ÑÐ¸Ð¸ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ñ Ð¸Ð¾Ð½Ð¾Ð² Ð¼Ð°Ð³Ð½Ð¸Ñ ÑÐ¾ÑÑÐ°Ð²Ð»ÑÐµÑ 20 Ð¼Ð. ÐÑÐ¸ ÑÑÐ¾Ð¼ Ð´Ð»Ñ Ð±ÐµÐ»ÐºÐ° FtsZ A. laidlawii Ð½Ð°Ð±Ð»ÑÐ´Ð°Ð»Ð¾ÑÑ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐ¸ ÑÐ±Ð¾ÑÐºÐ¸ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ¾Ð² Ð¿ÑÐ¸ ÑÐ°Ð·Ð½ÑÑ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸ÑÑ Ð¼Ð°Ð³Ð½Ð¸Ñ. Ð Ð±ÑÑÐµÑÐ°Ñ Ñ Ð²ÑÑÐ¾ÐºÐ¸Ð¼ ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸ÐµÐ¼ Ð¼Ð°Ð³Ð½Ð¸Ñ ÑÐ±Ð¾ÑÐºÐ° ÑÐ¸Ð»Ð°Ð¼ÐµÐ½ÑÐ¾Ð² Ð¿ÑÐ¾Ð¸ÑÑÐ¾Ð´Ð¸Ð»Ð° Ð¼ÐµÐ´Ð»ÐµÐ½Ð½ÐµÐµ.ÐÐ· Ð¾Ð¿Ð¸ÑÐ°Ð½Ð½ÑÑ Ð²ÑÑÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð² Ð¸ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ð°Ð½Ð¸Ð¸ Ð´Ð°Ð½Ð½ÑÑ Ð¸Ð· Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½ÑÑ Ð¸ÑÑÐ¾ÑÐ½Ð¸ÐºÐ¾Ð² Ð¾ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ ÐºÐ°Ð»Ð¸Ñ Ð¸ Ð±ÐµÐ»ÐºÐ° FtsZ Ð² ÐºÐ»ÐµÑÐºÐ°Ñ Ð¼Ð¸ÐºÐ¾Ð¿Ð»Ð°Ð·Ð¼ Ð¼Ð¾Ð¶Ð½Ð¾ Ð¿ÑÐµÐ´Ð¿Ð¾Ð»Ð¾Ð¶Ð¸ÑÑ, ÑÑÐ¾ Ð±ÐµÐ»Ð¾Ðº FtsZ A. laidlawii Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ¸Ð·ÑÐµÑÑÑ Ð² ÐºÐ»ÐµÑÐºÐµ. ÐÐ»Ñ M. gallisepticum Ð´Ð°Ð½Ð½ÑÐ¹ Ð²ÑÐ²Ð¾Ð´ ÑÐ´ÐµÐ»Ð°ÑÑ Ð·Ð°ÑÑÑÐ´Ð½Ð¸ÑÐµÐ»ÑÐ½Ð¾, Ð¿Ð¾ÑÐ¾Ð¼Ñ ÑÑÐ¾ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ñ ÐºÐ°Ð»Ð¸Ñ Ð² ÐºÐ»ÐµÑÐºÐµ Ð·Ð½Ð°ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾ Ð²ÑÑÐµ Ð¼Ð°ÐºÑÐ¸Ð¼Ð°Ð»ÑÐ½Ð¾Ð¹ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸, Ð¿ÑÐ¸ ÐºÐ¾ÑÐ¾ÑÐ¾Ð¹ Ð² Ð½Ð°ÑÐ¸Ñ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ñ Ð±ÐµÐ»Ð¾Ðº Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ¸Ð·Ð¾Ð²Ð°Ð»ÑÑ. ÐÐ»Ñ Ð²ÑÑÑÐ½ÐµÐ½Ð¸Ñ ÑÐ¾Ð»Ð¸ Ð³Ð¾Ð¼Ð¾Ð»Ð¾Ð³Ð¾Ð² Ð±ÐµÐ»ÐºÐ° FtsZ Ð² Ð¶Ð¸Ð·Ð½ÐµÐ½Ð½Ð¾Ð¼ ÑÐ¸ÐºÐ»Ðµ Ð¼Ð¸ÐºÐ¾Ð¿Ð»Ð°Ð·Ð¼ Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼Ð¾ Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐµÐµ Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ ÑÐ²Ð¾Ð¹ÑÑÐ² Ð´Ð°Ð½Ð½ÑÑ Ð³Ð¾Ð¼Ð¾Ð»Ð¾Ð³Ð¾Ð², Ð² ÑÐ¾Ð¼ ÑÐ¸ÑÐ»Ðµ Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ in vivo.
description: The given work is devoted to the study of the polymerization of the FtsZ proteins of Acholeplasma laidlawii and Mycoplasma gallisepticum depending on external conditions in vitro. The assembly of FtsZ filaments in the presence of GTP was detected using the 90Â° light scattering method.It has been noted that a high pH as well as a high concentration of potassium ions inhibit the polymerization of proteins. The optimal concentration of magnesium ions for polymerization is 20 mM. At the same time, for the A. laidlawii FtsZ protein, a change in the dynamics of polymers was observed at different concentrations of magnesium. In buffers with a high level of magnesium, the assembly of filaments occurs slower.It can be assumed that the A. laidlawii FtsZ protein can form filaments in the cell. This conclusion is based on the results described above and on the information from literature about the concentration of potassium and the FtsZ protein in mycoplasma cells. It is difficult to draw this conclusion for M. gallisepticum because the concentration of potassium in the cell is much higher than the maximum concentration at which we observed polymerization in our experiments. Further investigation, including in vivo conditions, is needed to elucidate the role of the FtsZ protein in the life cycle of mycoplasmas.