title: ÐÐ·ÑÑÐµÐ½Ð¸Ðµ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÐ¸ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð² ÑÐµÐºÑÐµÑÐ¾Ð¼Ð° ÑÑÐ°ÑÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°ÑÑ ÑÐµÐ½Ð¾ÑÐ¸Ð¿ ÑÑÐ°ÑÐµÐ½Ð¸Ñ Ð² Ð½Ð¾ÐºÐ°ÑÑÐ½ÑÑ Ð¿Ð¾ Ð³ÐµÐ½Ñ IGFBP3 Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÑ ÑÑÑÐ¾Ð¼Ð°Ð»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ñ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: The ability of secretome factors of old stem cells to induce the aging phenotype in IGFBP3-knockout mesenchymal stromal cells of the human endometrium
Creators: name: Ð Ð°Ð·Ð³ÑÐ»ÑÐµÐ²Ð°, ÐÐ°ÑÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸
Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÐµ ÑÑÑÐ¾Ð¼Ð°Ð»ÑÐ½ÑÐµ ÐºÐ»ÐµÑÐºÐ¸ ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ñ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°
IGFBP3 Ð½Ð¾ÐºÐ°ÑÑ
ÐºÐ¾Ð½Ð´Ð¸ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÑÐµÐ´Ð°
ÑÑÐ°ÑÐµÐ½Ð¸Ðµ ÐºÐ»ÐµÑÐ¾Ðº
mTOR ÑÐ¸Ð³Ð½Ð°Ð»ÑÐ½ÑÐ¹ Ð¿ÑÑÑ
Ñ Ð¾Ð½Ð´ÑÐ¾Ð³ÐµÐ½Ð½Ð°Ñ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐ°
stem cells
human endometrial mesenchymal stromal stem cells
IGFBP3 knockout
conditional environment
aging of cells
mTOR signal path
chondrogenic differentiation

description: Ð¦ÐµÐ»Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â Ð¿ÑÐ¾Ð°Ð½Ð°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°ÑÑ ÑÐµÐ½Ð¾ÑÐ¸Ð¿, Ð¿Ð¾ÑÐ²Ð»ÑÑÑÐ¸Ð¹ÑÑ Ð² ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ð°Ð»ÑÐ½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ Ñ Ð½Ð¾ÐºÐ°ÑÑÐ¾Ð¼ Ð¿Ð¾ Ð³ÐµÐ½Ñ IGFBP3 Ð¿Ð¾Ð´ Ð²Ð¾Ð·Ð´ÐµÐ¹ÑÑÐ²Ð¸ÐµÐ¼ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð² ÑÐµÐºÑÐµÑÐ¾Ð¼Ð° ÑÑÐ°ÑÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº. Ð¡ÑÐµÐ´Ð¸ Ð¼ÐµÑÐ¾Ð´Ð¾Ð², Ð¸ÑÐ¿Ð¾Ð»ÑÐ·ÑÐµÐ¼ÑÑ Ð² ÑÐ°Ð±Ð¾ÑÐµ: ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÐºÐ»ÐµÑÐ¾Ðº, ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÐ·, Ð¸Ð¼Ð¼ÑÐ½Ð¾Ð±Ð»Ð¾ÑÐ¸Ð½Ð³, SA-Î²-gal Ð¾ÐºÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ðµ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð², Ð¸Ð·Ð¼ÐµÑÐµÐ½Ð¸Ðµ ÑÐ°Ð·Ð¼ÐµÑÐ° ÐºÐ»ÐµÑÐºÐ¸, Ð¾ÐºÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ðµ Ð½Ð° Ð¿Ð¾Ð²ÐµÑÑÐ½Ð¾ÑÑÐ½ÑÐµ CD-Ð¼Ð°ÑÐºÐµÑÑ, Ð¾ÐºÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ðµ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð² Ð°Ð»ÑÐ¸Ð°Ð½Ð¾Ð²ÑÐ¼ ÑÐ¸Ð½Ð¸Ð¼, Ð¿Ð¾ÑÑÑÐ¾ÐµÐ½Ð¸Ðµ ÐºÑÐ¸Ð²Ð¾Ð¹ ÑÐ¾ÑÑÐ°, Ð°Ð½Ð°Ð»Ð¸Ð· ÑÐ°ÑÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ ÐºÐ»ÐµÑÐ¾Ðº Ð¿Ð¾ ÑÐ°Ð·Ð°Ð¼ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ ÑÐ¸ÐºÐ»Ð°. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð² ÑÐµÐºÑÐµÑÐ¾Ð¼Ð° ÑÑÐ°ÑÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð½Ð° ÑÐÐ¡Ð Ñ Ð½Ð¾ÐºÐ°ÑÑÐ¾Ð¼ Ð¿Ð¾ Ð³ÐµÐ½Ñ IGFBP3, Ð° ÑÐ°ÐºÐ¶Ðµ Ð±ÑÐ»Ð° Ð²ÑÑÐ²Ð»ÐµÐ½Ð° ÑÐ¿Ð¾Ð½ÑÐ°Ð½Ð½Ð°Ñ ÑÐ¾Ð½Ð´ÑÐ¾Ð³ÐµÐ½Ð½Ð°Ñ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²ÐºÐ° ÐºÐ»ÐµÑÐ¾Ðº. ÐÑÑÐµÐ¾Ð¿Ð¸ÑÐ°Ð½Ð½ÑÐµ ÑÐµÐ½Ð¾Ð¼ÐµÐ½Ñ ÑÐ¾ÑÑÐ°Ð²Ð»ÑÑÑ Ð½Ð°ÑÑÐ½ÑÑ Ð½Ð¾Ð²Ð¸Ð·Ð½Ñ Ð´Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ.
description: The aim of the study was to show the analyzing of the phenotype in the eMSC culture as a result of the effect of CMold factors on the cell culture. Among the methods used in the work: cell cultivation, electrophoresis, immunoblotting, SA-Î²-gal staining of cell preparations, measurement of cell size, staining for surface CD markers, staining of cell preparations with alcian blue, construction of a growth curve, analysis of the distribution of cells by phases of cell cycle. As a result of this work, the influence of secretome factors of old stem cells on eMSCs with knockout by the IGFBP3 gene was shown, and spontaneous chondrogenic differentiation of cells was also revealed. The above phenomena constitute the scientific novelty of the presented research.