Additional file 1 of Integration of National Health Insurance claims data and animal models reveals fexofenadine as a promising repurposed drug for Parkinson’s disease
Creators: name: Kim, Jae-Bong
name: Kim, Yujeong
name: Kim, Soo-Jeong
name: Ha, Tae‑Young
name: Kim, Dong-Kyu
name: Kim, Dong Won
name: So, Minyoung
name: Kim, Seung Ho
name: Woo, Hyun Goo
name: Yoon, Dukyong
name: Park, Sang Myun
Publisher: name: figshare
Year: 2024
Subjects: Medicine
Pharmacology
Biological Sciences not elsewhere classified
Information Systems not elsewhere classified
Cancer

description: Additional file 1: Figure S1. Schematic images of the regions annotated for pathology measures and used in subsequent analysis. Bolded regions were analyzed. Abbreviations : Cg = cingulate cortex; M1 = motor cortex 1; M2 = motor cortex 2; S1 = somatosensory cortex 1; S2 = somatosensory cortex 2; Ins = insular cortex; Pir = piriform cortex; Cpu = striatum; Sep = septal; Acb = accumbens nucleus; BST = bed nucleus of the stria terminalis; SI = substantia innominata; Hth = Hypothalamus; RS = retrosplenial cortex; PtA = parietal association cortex; Ect = ectorhinal cortex; PRh = perirhinal cortex; Ce = central amygdaloid nucleus; Me = medial amygdaloid nucelus; BLA = basolateral amygdaloid nucleus; BMA = basomedial amygdaloid nucelus; Thal = thalamic nuclei; Hipp = hippocampus; V = visual corterx; Au = auditory cortrex; TeA = temporal association cortex; Ent = entorhinal cortex; S = subiculum; SC = superior colliculus; MRN = midbrain red nucleus; MGN = medial geniculate nucleus; PAG = periaqueductal gray; VTA = ventral tegment area; SN = substantia nigra. Figure S2. Fexofenadine mitigates the astrogliosis in PFF-injected mice. IHC of GFAP in the ipsilateral S1, RS, Ect, BLA, Hipp, Thal, Ent, MRN, and PAG regions of PBS, PFF, and PFF + Fexofenadine (Fexo). Scale bar indicates 50 μm. Figure S3. Fexofenadine mitigates the microgliosis in PFF-injected mice. IHC of IBA-1 in the ipsilateral S1, RS, Ect, BLA, Hipp, Thal, Ent, MRN, and PAG regions of PBS, PFF, and PFF + Fexo. Scale bar indicates 50 μm. Figure S4. Fexofenadine alleviates the infiltration of CD3 positive T cells in PFF-injected mice. a IHC of CD3 in the ipsilateral and contralateral S2, RS, PRh, Thal, MRN, PAG, and SN regions of PBS, PFF, and PFF +Fexo. Scale bar indicates 50 μm. b Values are derived from the analysis of data collected from PBS (n = 3), PFF (n = 6), and PFF + Fexo (n = 4) groups. *** p &lt; 0.001, ** p &lt; 0.01, * p &lt; 0.05, two-way ANOVA. Figure S5. Fexofenadine does not affect macrophages in PFF-injected mice. a IHC of F4/80 in the ipsilateral and contralateral S2, Pir, RS, PRh, Thal, MRN, and PAG regions of PBS, PFF, and PFF + Fexo. Scale bar indicates 50 μm. b Values are derived from the analysis of data collected from PBS (n = 3), PFF (n = 4), and PFF + Fexo (n = 4) groups, two-way ANOVA. Figure S6. NK cell does not infiltrate in PFF-injected mice. IHC of NCR1 in the ipsilateral and contralateral M2, S2, Pir, Cpu, RS, Ect, BLA, Thal, V, MRN, PAG, and SN regions of PBS (n = 3), PFF (n = 4), and PFF + Fexo (n = 4). Scale bar indicates 50 μm. Table S1. Real-time PCR Primers. Table S2. Median time (in days) differences for each antihistamine.