title: ÐÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸ ÐºÐ¾Ð´Ð¸ÑÑÐµÐ¼Ð¾Ð³Ð¾ Ð±Ð¸Ð¾ÑÐµÐ½ÑÐ¾ÑÐ° HyPer Ð´Ð»Ñ Ð¾ÑÐµÐ½ÐºÐ¸ ÑÐ¸Ð¾Ð»ÑÐµÐ´ÑÐºÑÐ°Ð·Ð½Ð¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÑÐµÑÐ¼ÐµÐ½ÑÐ°ÑÐ¸Ð²Ð½Ð¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ñ ÑÐ¸Ð¾ÑÐµÐ´Ð¾ÐºÑÐ¸Ð½Ð° Ð¿ÑÐ¸ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ð¸ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: The usage of the genetically encoded HyPer biosensor to assess thiol reductase activi-ty of the thioredoxin enzyme system in the development of cell aging
Creators: name: ÐÐ¾Ð»Ð¾Ð²Ð¸Ð½Ð°, ÐÐ°ÑÑÐ°ÑÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð½ÑÐµ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸
Ð±Ð¸Ð¾ÑÐµÐ½ÑÐ¾Ñ HyPer
Ð¿ÑÐ¾ÑÐ¾ÑÐ½Ð°Ñ ÑÐ¸ÑÐ¾Ð¼ÐµÑÑÐ¸Ñ
ÑÐ¸Ð¾Ð»-Ð²Ð¾ÑÑÑÐ°Ð½Ð°Ð²Ð»Ð¸Ð²Ð°ÑÑÐ¸Ðµ ÑÐµÑÐ¼ÐµÐ½ÑÐ°ÑÐ¸Ð²Ð½ÑÐµ ÑÐ¸ÑÑÐµÐ¼Ñ
ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ðµ ÑÑÐ°ÑÐµÐ½Ð¸Ðµ
Ð³ÐµÐ½Ð¾ÑÐ¾ÐºÑÐ¸ÑÐµÑÐºÐ¸Ð¹ ÑÑÑÐµÑÑ
mesenchymal stem cells
HyPer biosensor
flow cytometry
thiol-reducing enzyme systems
replicating aging
genotoxic stress

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: ÐÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸ ÐºÐ¾Ð´Ð¸ÑÑÐµÐ¼Ð¾Ð³Ð¾ Ð±Ð¸Ð¾ÑÐµÐ½ÑÐ¾ÑÐ° HyPer Ð´Ð»Ñ Ð¾ÑÐµÐ½ÐºÐ¸ ÑÐ¸Ð¾Ð»ÑÐµÐ´ÑÐºÑÐ°Ð·Ð½Ð¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÑÐµÑÐ¼ÐµÐ½ÑÐ°ÑÐ¸Ð²Ð½Ð¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ñ ÑÐ¸Ð¾ÑÐµÐ´Ð¾ÐºÑÐ¸Ð½Ð° Ð¿ÑÐ¸ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ð¸ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ. Ð Ð°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¾ÑÐµÐ½ÐºÐµ ÑÐ¸Ð¾Ð»-ÑÐµÐ´ÑÐºÑÐ°Ð·Ð½Ð¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÑÐµÑÐ¼ÐµÐ½ÑÐ°ÑÐ¸Ð²Ð½Ð¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ñ ÑÐ¸Ð¾ÑÐµÐ´Ð¾ÐºÑÐ¸Ð½Ð° (Trx) Ð² ÑÐ¸ÑÐ¾Ð¿Ð»Ð°Ð·Ð¼Ðµ Ð¿Ð¾ÑÑÐ°ÑÐµÐ²ÑÐ¸Ñ Ð¼ÐµÐ·ÐµÐ½ÑÐ¸Ð¼Ð½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ (ÐÐ¡Ð) ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°. ÐÐ·Ð²ÐµÑÑÐ½Ð¾, ÑÑÐ¾ ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð°Ð»ÑÐ½Ð°Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐ½Ð¸Ð¶Ð°ÐµÑÑÑ Ð² ÑÑÐ°ÑÐµÑÑÐµÐ¼ Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ðµ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°, Ð¾Ð´Ð½Ð°ÐºÐ¾ Ð´Ð¾ ÑÐ¸Ñ Ð¿Ð¾Ñ Ð½ÐµÑÑÐµÐ½Ð¾, ÐºÐ°Ðº Ð¸Ð·Ð¼ÐµÐ½ÑÐµÑÑÑ ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð°Ð» ÑÐ¸Ð¾Ð»-Ð²Ð¾ÑÑÑÐ°Ð½Ð°Ð²Ð»Ð¸Ð²Ð°ÑÑÐ¸Ñ ÑÐ¸ÑÑÐµÐ¼ ÐºÐ»ÐµÑÐºÐ¸ Ð¿ÑÐ¸ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ð¸ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ. Ð Ð½Ð°ÑÑÐ¾ÑÑÐµÐ¹ ÑÐ°Ð±Ð¾ÑÐµ Ð±ÑÐ»Ð¾ Ð¿ÑÐµÐ´Ð»Ð¾Ð¶ÐµÐ½Ð¾ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°ÑÑ HyPer, ÑÐ¸Ð¾Ð»-ÑÐ¾Ð´ÐµÑÐ¶Ð°ÑÐ¸Ð¹ Ð³ÐµÐ½Ð½Ð¾Ð¸Ð½Ð¶ÐµÐ½ÐµÑÐ½ÑÐ¹ Ð±ÐµÐ»Ð¾Ðº, Ð±Ð¸Ð¾ÑÐµÐ½ÑÐ¾Ñ Ð2Ð2, Ð´Ð»Ñ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Trx-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾Ð¹ ÑÐ¸Ð¾Ð»-ÑÐµÐ´ÑÐºÑÐ°Ð·Ð½Ð¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð² ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÑÐµÐ¼ÑÑ ÐÐ¡Ð. ÐÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¾ÑÑ Ð´Ð²Ðµ Ð¼Ð¾Ð´ÐµÐ»Ð¸ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ: ÑÐµÐ¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð²Ð½Ð¾Ðµ Ð¸ ÑÑÑÐµÑÑ-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ðµ Ð¿ÑÐµÐ¶Ð´ÐµÐ²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ðµ ÑÑÐ°ÑÐµÐ½Ð¸Ðµ. Ð Ð¼Ð¾Ð»Ð¾Ð´ÑÑ Ð¸ Ð¿Ð¾ÑÑÐ°ÑÐµÐ²ÑÐ¸Ñ ÐÐ¡Ð, Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¼ÐµÑÐ¾Ð´Ð° Ð¿ÑÐ¾ÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐ¸ÑÐ¾Ð¼ÐµÑÑÐ¸Ð¸, Ð¾ÑÐµÐ½Ð¸Ð²Ð°Ð»Ð°ÑÑ ÑÐºÐ¾ÑÐ¾ÑÑÑ Ð²Ð¾ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¸Ñ ÑÐ¸Ð¾Ð»Ð¾Ð²ÑÑ Ð³ÑÑÐ¿Ð¿ HyPer Ð¿Ð¾ÑÐ»Ðµ Ð²Ð¾Ð·Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ð½Ð° ÐºÐ»ÐµÑÐºÐ¸ ÐºÐ¾ÑÐ¾ÑÐºÐ¾Ð³Ð¾ Ð¾ÐºÐ¸ÑÐ»Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ Ð¸Ð¼Ð¿ÑÐ»ÑÑÐ°. ÐÐ±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¾, ÑÑÐ¾ Ð°ÐºÑÐ¸Ð²Ð°ÑÐ¸Ñ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ñ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ Ð¿ÑÐ¸Ð²Ð¾Ð´Ð¸Ñ Ð² ÐÐ¡Ð Ðº ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ñ ÑÐ¸Ð¾Ð»-ÑÐµÐ´ÑÐºÑÐ°Ð·Ð½Ð¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Trx Ð² ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐ¸ÑÐ¾Ð¿Ð»Ð°Ð·Ð¼Ðµ. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ Ð² ÑÐ°Ð±Ð¾ÑÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ ÑÐ°ÑÑÐ¸ÑÑÑÑ ÑÑÑÐµÑÑÐ²ÑÑÑÐ¸Ðµ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÐµÐ½Ð¸Ñ Ð¾ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½ÑÑ Ð°ÑÐ¿ÐµÐºÑÐ°Ñ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ ÑÑÐ°ÑÐµÐ½Ð¸Ñ, Ð° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð½ÑÐ¹ Ð² ÑÐ°Ð±Ð¾ÑÐµ Ð¼ÐµÑÐ¾Ð´ Ð¼Ð¾Ð¶ÐµÑ Ð±ÑÑÑ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½ Ð² Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐµÐ¼ Ð´Ð»Ñ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ¸ ÑÐ¸Ð¾Ð»-ÑÐµÐ´ÑÐºÑÐ°Ð·Ð½ÑÑ ÑÐ¸ÑÑÐµÐ¼ ÐºÐ»ÐµÑÐ¾Ðº Ð² ÑÐ»ÑÑÐ°Ðµ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ Ð½Ð°ÑÑÑÐµÐ½Ð¸Ð¹ ÑÐµÐ´Ð¾ÐºÑ-Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¼Ð°, Ð¾Ð¿Ð¾ÑÑÐµÐ´Ð¾Ð²Ð°Ð½Ð½ÑÑ Ð¿Ð°ÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐ¼Ð¸ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°.
description: The subject of the graduate qualification work is: Â«The usage of the genetically encoded HyPer biosensor to assess thiol reductase activity of the thioredoxin enzyme system in the development of cell agingÂ». The given work is devoted to the assessment of thiol reductase activity of the thioredoxin (Trx) enzyme system in the cytoplasm of aged human mesenchymal stem cells (MSCs). Stem cell activity decreases in aging humans. The contribution of thiol-regenerating cell systems to the processes of age-related degeneration and how the functionality of these systems changes during development of cell aging is still unclear. The thiol-containing genetically engineered protein HyPer is restored in eukaryotic cells by the Trx system. In the present work, we proposed to use HyPer to analyze Trx-dependent thiol reductase activity in cultured MSCs. Two models of cell aging were used: replicative and premature aging. To assess the functional activity of the Trx system in young and aged MSCs using flow cytometry we evaluated the rate of thiol group HyPer reduction after a short oxidative pulse was applied to the cells. It was found that activation of the cell aging program leads to a decrease in thiol reductase activity in the cell cytoplasm of MSCs.The results obtained in this work extend the existing understanding of molecular aspects of cell aging, while the method of Trx system activity assessment used in this work can be further applied to characterize thiol reductase systems of cells in case of various disorders of redox metabolism mediated by human pathologies.