title: Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð¸ Ð²Ð°Ð»Ð¸Ð´Ð°ÑÐ¸Ñ Ð¼ÐµÑÐ¾Ð´Ð¸Ðº Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¿ÐµÑÐ¾ÑÐ°Ð»ÑÐ½ÑÑ Ð°Ð½ÑÐ¸ÐºÐ¾Ð°Ð³ÑÐ»ÑÐ½ÑÐ¾Ð² Ð´Ð»Ñ ÐºÐ»Ð¸Ð½Ð¸ÑÐµÑÐºÐ¸Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Development and validation of methods for the determination of oral anticoagulants for clinical trials
Creators: name: ÐÑÑÐ¸Ð½Ð°, ÐÐ»ÐµÐ½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÐÐ¸Ð¾ÑÐµÑ Ð½Ð¾Ð»Ð¾Ð³Ð¸Ñ
Ð¿ÐµÑÐ¾ÑÐ°Ð»ÑÐ½ÑÐµ Ð°Ð½ÑÐ¸ÐºÐ¾Ð°Ð³ÑÐ»ÑÐ½ÑÑ
Ð²Ð°Ð»Ð¸Ð´Ð°ÑÐ¸Ñ
ÑÐ°ÑÐ¼Ð°ÐºÐ¾ÐºÐ¸Ð½ÐµÑÐ¸ÐºÐ°
ÑÑÐ¾Ð¼Ð±Ð¾ÑÐ¼Ð±Ð¾Ð»Ð¸Ñ
oral anticoagulants
validation
pharmacokinetics
tromboembolism

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description: The subject of graduate qualification work is: Â«Development and validation of methods for the determination of oral anticoagulants for clinical trialsÂ». Due to the disease with a new coronavirus infection, patients experienced a deterioration in blood circulation due to formed blood clots. For the treatment of this disease, anticoagulants are used, drugs that reduce the activity of blood clotting. The purpose of this work was to develop and validate methods for the quantitative determination of new oral anticoagulants. The article presents a method of high-performance liquid chromatography with tandem mass spectrometry. The process of selection of chromatography conditions and sample preparation of samples is described. Methods for the determination of oral anticoagulants have been developed: dabigatran, apixaban and rivaroxaban. As a result of the experiments, it was shown that the use of liquid-liquid extraction makes it possible to effectively extract apixaban and rivaroxaban from the biological matrix, and by protein deposition to effectively extract dabigatran. The work was carried out based on the Laboratory of the Â«Analytical SpectrometryÂ», where was worked out the methods of determination anticoagulants.