title: Ð¡Ð¾Ð·Ð´Ð°Ð½Ð¸Ðµ Ð²ÑÑÐ¾ÐºÐ¾ÑÑÐ²ÑÑÐ²Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÐµÑÑ-ÑÐ¸ÑÑÐµÐ¼Ñ Ð´Ð»Ñ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ñ Ð³ÐµÐ½Ð¾ÑÐ¾ÐºÑÐ¸ÐºÐ°Ð½ÑÐ¾Ð² Ð² Ð¾ÐºÑÑÐ¶Ð°ÑÑÐµÐ¹ ÑÑÐµÐ´Ðµ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Creating a highly sensitive test system for analyzing the content of genotoxicants in the environment
Creators: name: Ð¤ÐµÐ´Ð¾ÑÐ¾Ð², ÐÐ»ÐµÐºÑÐ°Ð½Ð´Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: ÐÐºÑÑÐ¶Ð°ÑÑÐ°Ñ ÑÑÐµÐ´Ð°
Ð³ÐµÐ½Ð¾ÑÐ¾ÐºÑÐ¸ÐºÐ°Ð½ÑÑ
ÑÐ¿Ð¾Ð½ÑÐ°Ð½Ð½ÑÐ¹ Ð¼ÑÑÐ°Ð³ÐµÐ½ÐµÐ·
ÑÐµÑÑ-ÑÐ¸ÑÑÐµÐ¼Ð°
Ð´ÐµÐ»ÐµÑÐ¸Ñ
Ð´ÑÐ¾Ð¶Ð¶Ð¸
Saccharomyces cerevisiae
hsm3
rad2.
genotoxicants
spontaneous mutagenesis
test system
deletion
yeast

description: ÐÐ° 53Ñ., 10 ÑÐ¸Ñ., 4 ÑÐ°Ð±Ð». Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«Ð¡Ð¾Ð·Ð´Ð°Ð½Ð¸Ðµ Ð²ÑÑÐ¾ÐºÐ¾ÑÑÐ²ÑÑÐ²Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÐµÑÑ-ÑÐ¸ÑÑÐµÐ¼Ñ Ð´Ð»Ñ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ñ Ð³ÐµÐ½Ð¾ÑÐ¾ÐºÑÐ¸ÐºÐ°Ð½ÑÐ¾Ð² Ð² Ð¾ÐºÑÑÐ¶Ð°ÑÑÐµÐ¹ ÑÑÐµÐ´ÐµÂ». ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð³ÐµÐ½Ð¾ÑÐ¾ÐºÑÐ¸ÐºÐ°Ð½ÑÐ¾Ð² Ð½Ð° ÑÑÐ°Ð¼Ð¼ Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹ Ñ ÑÐ½Ð¸ÐºÐ°Ð»ÑÐ½ÑÐ¼Ð¸ Ð¼ÑÑÐ°ÑÐ¸ÑÐ¼Ð¸ Ð¸ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾ÑÑÐ¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ñ Ð´Ð°Ð½Ð½Ð¾Ð³Ð¾ ÑÑÐ°Ð¼Ð¼Ð° ÐºÐ°Ðº ÑÐµÑÑ Ð½Ð° Ð¿ÑÐ¸ÑÑÑÑÑÐ²Ð¸Ðµ Ð³ÐµÐ½Ð¾ÑÐ¾ÐºÑÐ¸ÐºÐ°Ð½ÑÐ¾Ð² Ð² Ð¾ÐºÑÑÐ¶Ð°ÑÑÐµÐ¹ ÑÑÐµÐ´Ðµ. ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: 1. Ð¡Ð¾Ð·Ð´Ð°Ð½Ð¸Ðµ ÑÑÐ°Ð¼Ð¼Ð° Ñ Ð¾Ð´Ð¸Ð½Ð¾ÑÐ½Ð¾Ð¹ Ð¼ÑÑÐ°ÑÐ¸ÐµÐ¹ rad2â. 2. Ð¡Ð¾Ð·Ð´Ð°Ð½Ð¸Ðµ ÑÑÐ°Ð¼Ð¼Ð° Ñ Ð¾Ð´Ð¸Ð½Ð¾ÑÐ½Ð¾Ð¹ Ð¼ÑÑÐ°ÑÐ¸ÐµÐ¹ hsm3â. 3. Ð¡Ð¾Ð·Ð´Ð°Ð½Ð¸Ðµ ÑÑÐ°Ð¼Ð¼Ð° Ñ Ð´Ð²Ð¾Ð¹Ð½Ð¾Ð¹ Ð¼ÑÑÐ°ÑÐ¸ÐµÐ¹ rad2âhsm3â. 4. ÐÐ·ÑÑÐµÐ½Ð¸Ðµ ÑÑÐ²ÑÑÐ²Ð¸ÑÐµÐ»ÑÐ½Ð¾ÑÑÐ¸ Ð²ÑÐµÑ ÑÐ¸Ð½ÑÐµÐ·Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ ÑÐµÑÑÐµÑÐ¾Ð² Ðº Ð¼ÑÑÐ°Ð³ÐµÐ½Ð½Ð¾Ð¼Ñ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ð¼ÐµÑÐ¸Ð»Ð¼ÐµÑÐ°Ð½ÑÑÐ»ÑÑÐ¾Ð½Ð°ÑÐ°, ÑÑÐ¸Ð»Ð¼ÐµÑÐ°Ð½ÑÑÐ»ÑÑÐ¾Ð½Ð°ÑÐ°, Ð¿ÐµÑÐµÐºÐ¸ÑÐ¸ Ð²Ð¾Ð´Ð¾ÑÐ¾Ð´Ð°. Ð Ð°Ð±Ð¾ÑÐ° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ Ð¤ÐÐÐ£ "ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¸Ð½ÑÑÐ¸ÑÑÑ ÑÐ´ÐµÑÐ½Ð¾Ð¹ ÑÐ¸Ð·Ð¸ÐºÐ¸ Ð¸Ð¼. Ð. Ð. ÐÐ¾Ð½ÑÑÐ°Ð½ÑÐ¸Ð½Ð¾Ð²Ð° ÐÐ°ÑÐ¸Ð¾Ð½Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÑÐºÐ¾Ð³Ð¾ ÑÐµÐ½ÑÑÐ° "ÐÑÑÑÐ°ÑÐ¾Ð²ÑÐºÐ¸Ð¹ Ð¸Ð½ÑÑÐ¸ÑÑÑ", Ð³Ð´Ðµ ÑÐ¾Ð±Ð¸ÑÐ°Ð»Ð°ÑÑ Ð·Ð½Ð°ÑÐ¸ÑÐµÐ»ÑÐ½Ð°Ñ ÑÐ°ÑÑÑ ÑÐ°ÐºÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ð°. ÐÑÐ»Ð¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ñ ÑÐ°ÑÑÐµÑÑ, Ð¿Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÑÑÐ¸Ðµ Ð½Ð°Ð³Ð»ÑÐ´Ð½Ð¾, ÐºÐ°ÐºÐ¸Ð¼ Ð¾Ð±ÑÐ°Ð·Ð¾Ð¼ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ñ ÑÐµÑ Ð¸Ð»Ð¸ Ð¸Ð½ÑÑ Ð¼ÑÑÐ°Ð³ÐµÐ½Ð¾Ð² Ð²Ð»Ð¸ÑÐµÑ Ð½Ð° ÑÐ°ÑÑÐ¾ÑÑ Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ Ð¼ÑÑÐ°ÑÐ¸Ð¹. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð±ÑÐ»Ð¾ Ð´Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ ÑÑÐ°Ð¼Ð¼ Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹ Saccharomyces cerevisiae Ñ Ð´ÐµÐ»ÐµÑÐ¸ÐµÐ¹ Ð¿Ð¾ Ð³ÐµÐ½Ð°Ð¼ RAD2 Ð¸ HSM3 ÑÐ²ÐµÑÑÑÑÐ²ÑÑÐ²Ð¸ÑÐµÐ»ÐµÐ½ Ðº Ð²Ð¾Ð·Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ Ð³ÐµÐ½Ð¾ÑÐ¾ÐºÑÐ¸ÐºÐ°Ð½ÑÐ¾Ð², ÐºÐ°Ðº Ð½Ð°Ð¿ÑÐ¸Ð¼ÐµÑ Ð¼ÐµÑÐ¸Ð»Ð¼ÐµÑÐ°Ð½ÑÑÐ»ÑÑÐ¾Ð½Ð°ÑÐ°, ÑÑÐ¸Ð»Ð¼ÐµÑÐ°Ð½ÑÑÐ»ÑÑÐ¾Ð½Ð°ÑÐ° Ð¸ Ð¿ÐµÑÐµÐºÐ¸ÑÐ¸ Ð²Ð¾Ð´Ð¾ÑÐ¾Ð´Ð°, Ð¾ ÑÐµÐ¼ ÑÐ²Ð¸Ð´ÐµÑÐµÐ»ÑÑÑÐ²ÑÐµÑ ÑÐµÐ·ÐºÐ¾Ðµ ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ðµ ÑÐ°ÑÑÐ¾ÑÑ ÑÐ¿Ð¾Ð½ÑÐ°Ð½Ð½ÑÑ Ð¼ÑÑÐ°ÑÐ¸Ð¹ Ð¿ÑÐ¸ Ð¼Ð¸Ð½Ð¸Ð¼Ð°Ð»ÑÐ½ÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·ÑÐµÐ¼ÑÑ Ð´Ð¾Ð·Ð°Ñ Ð¼ÑÑÐ°Ð³ÐµÐ½Ð¾Ð², Ð¸ ÐµÐ³Ð¾ Ð¼Ð¾Ð¶Ð½Ð¾ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°ÑÑ ÐºÐ°Ðº ÑÐµÑÑ Ð½Ð° Ð¿ÑÐ¸ÑÑÑÑÑÐ²Ð¸Ðµ Ð³ÐµÐ½Ð¾ÑÐ¾ÐºÑÐ¸ÐºÐ°Ð½ÑÐ¾Ð² Ð² Ð¾ÐºÑÑÐ¶Ð°ÑÑÐµÐ¹ ÑÑÐµÐ´Ðµ.
description: 53 pages, 10 figures, 4 tables The theme of the final qualification work: "Creating a highly sensitive test system for analyzing the content of genotoxicants in the environment." This work is devoted to the study of the influence of genotoxicants on a strain of yeast with unique mutations and the possibility of using this strain as a test for the presence of genotoxicants in the environment. Tasks that were solved during the study: 1. Creation of a strain with a single mutation rad2Î. 2. Creation of a strain with a single mutation hsm3Î. 3. Creation of a double mutation strain rad2Îhsm3Î. 4. The study of the sensitivity of all synthesized testers to the mutagenic effect of methyl methanesulfonate, ethyl methanesulfonate, hydrogen peroxide. The work was carried out on the basis of the FSBI Petersburg Institute of Nu-clear Physics of the Kurchatov Institute National Research CenterÂ», where a significant part of the actual material was collected. Calculations were made that clearly show how concentration of certain mutagens affects on the frequency of formation of induced mutations. As a result, it was proved that the strain of yeast Saccharomyces cerevisiae with a deletion of the RAD2 and HSM3 genes is hypersensitive to the effects of various genotoxicants, such as methyl methanesulfonate, ethyl methanesulfonate and hydro-gen peroxide, as evidenced by a sharp increase in the frequency of spontaneous mutations at the lowest used doses of mutagens, and it can be used as a test for the presence of genotoxicants in the environment.