MOESM1 of Engineered Thermoanaerobacterium aotearoense with nfnAB knockout for improved hydrogen production from lignocellulose hydrolysates
Creators: name: Li, Yang
name: Hu, Jialei
name: Qu, Chunyun
name: Chen, Lili
name: Guo, Xiaolong
name: Fu, Hongxin
name: Wang, Jufang
Publisher: name: figshare
Year: 2019
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Pharmacology
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Inorganic Chemistry
subject: FOS: Chemical sciences
subject: Science Policy
subject: Hematology
subject: Plant Biology

description: Additional file 1: Fig. S1. Verification of nfnAB deletion mutants. (A) PCR amplification analysis of nfnAB deletion mutants and their parental strains. M: DL5,000 DNA ladder; lane 1 and lane 2: SCUT27/∆nfnAB; lane 3 and lane 4: SCUT27. (B) Southern blots of nfnAB deletion mutant and the parental strains. M: DNA molecular weight marker; lane 1: vector pBlunAB; lane 2 and lane 3: SCUT27; lane 4 and lane 5: SCUT27/∆nfnAB. Table S1. Hydrogen production and sugar use of SCUT27 with glucose, xylose and glucose/xylose mixture. Table S2. Hydrogen production and sugar use of SCUT27/∆nfnAB with glucose, xylose and glucose/xylose mixture. Table S3. The effect of CaCO3 addition on products distribution (mol/mol glucose) for SCUT27 and SCUT27/∆nfnAB. Table S4. Concentration of arabinose, mannose and galactose in different hydrolysates. Table S5. Composition of various dilute acid-pretreated lignocellulose hydrolysates. Table S6. Raw data for fermentation of SCUT27 using rice straw hydrolysate in serum bottles. Table S7. Raw data for fermentation of SCUT27/∆nfnAB using rice straw hydrolysate in serum bottles. Table S8. Raw data for fermentation of SCUT27 using corn cob hydrolysate in serum bottles. Table S9. Raw data for fermentation of SCUT27/∆nfnAB using corn cob hydrolysate in serum bottles. Table S10. Raw data for fermentation of SCUT27 using corn straw hydrolysate in serum bottles. Table S11. Raw data for fermentation of SCUT27/∆nfnAB using corn straw hydrolysate in serum bottles. Table S12. Raw data for fermentation of SCUT27 using soybean straw hydrolysate in serum bottles. Table S13. Raw data for fermentation of SCUT27/∆nfnAB using soybean straw hydrolysate in serum bottles. Table S14. Raw data for fermentation of SCUT27 using wheat straw hydrolysate in serum bottles. Table S15. Raw data for fermentation of SCUT27/∆nfnAB using wheat straw hydrolysate in serum bottles. Table S16. Raw data for fermentation of SCUT27 using sorghum straw hydrolysate in serum bottles. Table S17. Raw data for fermentation of SCUT27/∆nfnAB using sorghum straw hydrolysate in serum bottles. Table S18. Raw data for fermentation of SCUT27/∆nfnAB using sterilized rice straw hydrolysate in 5-L bioreactor. Table S19. Raw data for fermentation of SCUT27/∆nfnAB using nonsterilized rice straw hydrolysate in 5-L bioreactor. Table S20. Raw data for fermentation of SCUT27/∆nfnAB using sterilized corn cob hydrolysate in 5-L bioreactor. Table S21. Raw data for fermentation of SCUT27/∆nfnAB using nonsterilized corn cob hydrolysate in 5-L bioreactor. Table S22. Effect of yeast extract concentration on sugar consumption and product distribution (mol/mol substrate) of T. aotearoense SCUT27.