cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Explicit Analytic Solutions for the Subsurface Stress Field in Single Plane Contacts of Elastically Similar Truncated Cylinders or Wedges
Creators: name: Willert, Emanuel
Publisher: name: Technische Universität Berlin
Year: 2022
Subjects: subject: 620 Ingenieurwissenschaften und zugeordnete Tätigkeiten
subject: plane contacts
subject: subsurface stresses
subject: truncated indentors
subject: Muskhelishvili potential
subject: Ciavarella–Jäger principle
subject: flat punch superposition

description: As has been pointed out recently, a possible solution strategy to the wear–fatigue dilemma in fretting, operating on the level of contact mechanics and profile geometries, can be the introduction of “soft” sharp edges to the contact profiles, for example, by truncating an originally smooth profile. In that regard, analysis of possible mechanical failure of a structure, due to the contact interaction, requires the knowledge of the full subsurface stress state resulting from the contact loading. In the present manuscript, a closed-form exact solution for the subsurface stress state is given for the frictional contact of elastically similar truncated cylinders or wedges, within the framework of the half-plane approximation and a local-global Amontons–Coulomb friction law. Moreover, a fast and robust semi-analytical method, based on the appropriate superposition of solutions for parabolic contact, is proposed for the determination of the subsurface stress fields in frictional plane contacts with more complex profile geometries, and compared with the exact solution. Based on the analytical solution, periodic tangential loading of a truncated cylinder is considered in detail, and important scalar characteristics of the stress state, like the von-Mises equivalent stress, maximum shear stress, and the largest principal stress, are determined. Positive (i.e., tensile) principal stresses only exist in the vicinity of the contact edge, away from the pressure singularity at the edge of the profile, and away from the maxima of the von-Mises equivalent stress, or the maximum shear stress. Therefore, the fretting contact should not be prone to fatigue crack initiation.
title: Das nasale chondro-mesenchymale Hamartom (NCMH) – eine seltene Ursache für Nasenatmungsbehinderung im Kindesalter
Creators: name: Brüsseler, Melanie
name: Stenin, Igor
name: Plettenberg, Christian
name: Schipper, Jörg
name: Wagenmann, Martin
Publisher: name: German Medical Science GMS Publishing House
Year: 2019
Subjects: subject: 610 Medical sciences; Medicine

description: Einleitung: Chondromesenchymale Hamartome der Nase (NCMH) sind seltene, benigne Tumore, welche erstmalig von McDermott et al beschrieben wurden. NCMH treten vermehrt im Kindesalter auf. In der Literatur sind nur ca. 50 Fälle beschrieben. Häufiges Erstsymptom ist die Nasenatmungsbehinderung[zum vollständigen Text gelangen Sie über die oben angegebene URL]
description: Jahrestagung der Vereinigung Westdeutscher Hals-Nasen-Ohren-Ärzte
The mass spectra and decay properties of dimesonic states, using the Hellmann potential
Creators: name: Rai, Ajay Kumar
name: Rathaud, D. P.
Publisher: name: arXiv
Year: 2015
Subjects: subject: High Energy Physics - Phenomenology (hep-ph)
subject: FOS: Physical sciences

description: Mass spectra of the dimesonic (meson-antimeson) molecular states are computed using the Hellmann potential in variational approach, which consists of relativistic correction to kinetic energy term as well as to the potential energy term. For the study of molecular bound state system, the Hellmann potential of the form $V(r)=-\frac{α_{s}}{r} + \frac{B e^{-Cr}}{r}$ is being used. The one pion exchange potential (OPEP) is also incorporated in the mass calculation. The digamma decay width and decay width of the dimesonic system are evaluated using the wave function. The experimental states such as $f_{0}(980)$, $b_{1}(1235)$, $h_{1}(1380)$, $a_{0}(1450)$, $f_{0}(1500)$, $f_{2}'(1525)$,$f_{2}(1565)$, $h_{1}(1595)$, $a_{2}(1700)$, $f_{0}(1710)$, $f_{2}(1810)$ are compared with dimesonic states. Many of these states (masses and their decay properties) are close to our theoretical predictions.
description: 11 pages, 4 tables
title: Ballondilatation der Eustachischen Röhre (BET) – eine histopathologische Untersuchung
Creators: name: Christov, Florian
name: Gluth, Michael
name: Arweiler-Harbeck, Diana
name: Lang, Stephan
Publisher: name: German Medical Science GMS Publishing House
Year: 2019
Subjects: subject: 610 Medical sciences; Medicine

description: Einleitung: Die Tubendilatation (BET) wird immer häufiger zur Verbesserung der Tubenfunktion, u.a. bei chronischem Paukenerguss (OME), angewendet. Trotz tendenziell optimistischer Studienlage gibt es Patienten, die langfristig nicht von einer BET profitieren. Ziel der Untersuchung war es, anhand[zum vollständigen Text gelangen Sie über die oben angegebene URL]
description: Jahrestagung der Vereinigung Westdeutscher Hals-Nasen-Ohren-Ärzte
Ethanol-induced GABAA receptor alpha4 subunit plasticity involves phosphorylation and neuroactive steroids
Creators: name: Werner, David F.
name: O'Buckley, Todd K.
name: Kumar, Sandeep
name: Porcu, Patrizia
name: Morrow, A. Leslie
name: Carter, Jenna M.
name: Boyd, Kevin N.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2016

description: GABAA receptors containing α4 subunits are widely implicated in acute ethanol sensitivity, and their spatial and temporal regulation prominently contributes to ethanol-induced neuroplasticity in hippocampus and cortex. However, it is unknown if α4-containing GABAA receptors in the thalamus, an area of high α4 expression, display similar regulatory patterns following ethanol administration, and if so, by which molecular mechanisms. In the current study, thalamic GABAA receptor α4 subunit levels were increased following a 6-week, but not a 2-week chronic ethanol diet. Following acute high-dose ethanol administration, thalamic GABAA receptor α4 subunit levels were regulated in a temporal fashion, as a decrease was observed at 2 hours followed by a delayed transient increase. PKCγ and PKCδ levels paralleled α4 temporal expression patterns following ethanol exposure. Initial decreases in α4 subunit expression were associated with reduced serine phosphorylation. Delayed increases in expression were not associated with a change in phosphorylation state, but were prevented by inhibiting neuroactive steroid production with the 5α-reductase inhibitor finasteride. Overall, these studies indicate that thalamic GABAA receptor α4 subunit expression following acute and chronic ethanol administration exhibits similar regulatory patterns as other regions and that transient expression patterns following acute exposure in vivo are likely dependent on both subunit phosphorylation state and neuroactive steroids.
title: Postoperative Schmerzen als Indikator einer drohenden subjektiven Nachblutung im HNO-Fachbereich
Creators: name: Beule, Achim
name: Schaller, Marlene
name: Thiele, Thomas
name: Kohlmann, Thomas
name: Hosemann, Werner
name: Rudack, Claudia
name: Scharf, Christian
Publisher: name: German Medical Science GMS Publishing House
Year: 2019
Subjects: subject: 610 Medical sciences; Medicine

description: Einleitung: Krankenhäuser intensivieren die postoperative Überwachung der Schmerzen z.B. mit Hilfe von visuellen Analogskalen. Diese häufig durch Mitarbeiter der Pflege erhobenen Daten werden bislang ausschließlich genutzt, um die Gabe von Analgetika individuell zu steuern. Eine [zum vollständigen Text gelangen Sie über die oben angegebene URL]
description: Jahrestagung der Vereinigung Westdeutscher Hals-Nasen-Ohren-Ärzte
ปัจจัยด้านจิตวิทยาที่มีผลต่อการตัดสินใจซื้อสินค้าที่ติดฉลากลดโลกร้อนในประเทศไทย
Creators: name: วรินทร์ธร โตพันธ์
Publisher: name: คณะบริหารธุรกิจ เศรษฐศาสตร์และการสื่อสาร มหาวิทยาลัยนเรศวร
Year: 2560
Subjects: พฤติกรรมการบริโภคสีเขียว
ฉลากลดโลกร้อน
ปัจจัยด้านจิตวิทยา
การตัดสินใจซื้อ
Green Consumption Behavior
Carbon Footprint Reduction Label
Psychological Factors
Purchasing Decision

description: วารสารบริหารธุรกิจ เศรษฐศาสตร์และการสื่อสาร, 12, 2, 119-132
Hepatoprotective effect of Antrodia Cinnamomea mycelia extract in subhealth Japanese adults: a randomized, double-blind, placebo-controlled clinical study
Creators: name: Ho, Chun-Yi
name: Kuan, Chen-Meng
name: Hsu, Pang-Kuei
Publisher: name: Taylor & Francis
Year: 2022
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Pharmacology
subject: Chemical Sciences not elsewhere classified
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Science Policy
subject: Mental Health

description: Antrodia cinnamomea, a unique Taiwanese fungus (mushroom), has demonstrated the hepatoprotective activities in animals with liver injury. Nevertheless, there are few studies reporting the efficacy of the fungus in subhealth subjects (alanine aminotransferase (ALT) levels between 31 and 50 U/L and aspartate aminotransferase (AST) levels ≤ 50 U/L). In this study, we assessed the ameliorating effect of a A. cinnamomea mycelia extract (ACME) on liver health in asymptomatic individuals with marginally high ALT levels. Forty-four eligible Japanese adults were enrolled in this randomized, double-blind, placebo-controlled clinical study and instructed to take an ACME capsule (250 mg of ACME powder) or a placebo capsule daily for 12 weeks. The primary outcomes (i.e. ALT and AST) were analyzed at 0, 4, 8, and 12 weeks. No treatment-related adverse effects were observed throughout this study. In efficacy analyses with the per-protocol (PP) cohort of participants, there were no significant changes in ALT and AST levels within and between groups. However, subgroup analysis showed that ACME could significantly improve the mean ALT level of regular drinkers, consuming alcoholic drinks more than twice a week, after the study in comparison with the result of the placebo group. This exploratory study indicated that the ACME might effectively improve liver health in regular drinkers.
title: The Shape of a Language
Creators: name: Plescia, Iolanda
name: Culpeper, Jonathan
name: Hope, Jonathan
name: Ingham, Michael
name: Ingham, Richard
name: Mullini, Roberta
name: Ranzato, Irene
name: McDonald, Russ
name: Fusini, Nadia
name: Colombo, Rosy
name: Roccati, Alessandro
Publisher: name: Sapienza Università Editrice
Year: 2016
Subjects: Editor’s Foreword
The Shape of Early Modern English: An Interview with Jonathan Culpeper on the Encyclopaedia of Shakespeare’s Language Project
Who Invented ‘Gloomy’? Lies People Want to Believe about Shakespeare
Comparing Syntactic Strategies for Proximity and Distance in the Verse/Prose Comedies of Shakespeare and Jonson
The Pragmatics of Dialogical Asides in Shakespeare
“Danes Do It Melancholy”: Allusions to Shakespeare in Films and TV
Come into the Garden, Bard; Or, From Bed to Verse
Rome Desired; Or, The Idea of Rome
Back from the Dead. An Encounter with Domitius Enobarbus

description: Memoria di Shakespeare vol. 3
ปัจจัยที่มีอิทธิพลต่อความพึงพอใจในงาน ผลการปฏิบัติงานและความต้องการออกจากงานของมัคคุเทศก์
Creators: name: สาวิตรี ยอยยิ้ม
Publisher: name: คณะบริหารธุรกิจ เศรษฐศาสตร์และการสื่อสาร มหาวิทยาลัยนเรศวร
Year: 2560
Subjects: ความเครียดในการทำงาน
ความขัดแย้งในครอบครัวที่มีสาเหตุมาจากงาน
ความพึงพอใจในงาน
ผลการปฏิบัติงาน
ความต้องการออกจากงาน
Job stress
Work conflict as from job role
Job Satisfaction
Job performance
Intention to leave

description: วารสารบริหารธุรกิจ เศรษฐศาสตร์และการสื่อสาร, 12, 2, 133-144
Medicinal Chemistry for Novel Treatments for Visceral Leishmaniasis
Creators: name: ZHENG, REBECCA JIAWEN
Publisher: name: Monash University
Year: 2022
Subjects: Pharmaceutical sciences

description: Visceral leishmaniasis is of growing concern globally and affects approximately 12 million people. This thesis investigates the structure-activity relationship of two scaffolds against L. donovani in an attempt to uncover novel treatments for visceral leishmaniasis. Chapter 1 provides an overall background surrounding the disease and current treatments. Chapters 2 to 4 discuss the work conducted throughout the research project and provide the results of this work. Chapter 5 contains the experimental data.
title: Messung des betriebswirtschaftlichen Erfolgs von Nachwuchsleistungszentren im deutschen Profifußball
title: Measuring the business success of youth academies in German professional soccer : development and application of an innovative evaluation concept
titleType: AlternativeTitle
Creators: name: Honsel, Martina
Publisher: name: Universitätsbibliothek Siegen
Year: 2023
Subjects: subject: 330 Wirtschaft
subject: Evaluation
subject: Qualität
subject: betriebswirtschaftlicher Erfolg
subject: Nachwuchsleistungszentrum
subject: Fußball
subject: evaluation
subject: quality
subject: business success
subject: youth development center
subject: soccer
subject: Evaluation
subject: Nachwuchs
subject: Fußball
subject: Talentförderung
subject: Sportförderung

description: Fußball ist eine beliebte Sportart, die weltweit Millionen von Menschen begeistert. Über Fußball lässt sich vortrefflich diskutieren, über aktuelle Spiele ebenso wie über Taktiken oder Spielertransfers. Die Bedeutung der Fähigkeiten der Spieler im Profifußball ist enorm, ihre Ausbildung für den Profifußball in Deutschland ist ein wichtiger Prozess, der in den Nachwuchsleistungszentren der Vereine stattfindet. Diese Jugendakademien ermöglichen talentierten jungen Spielern, ihre fußballerischen Fähigkeiten zu entwickeln und sich auf eine mögliche Karriere im Profifußball vorzubereiten. Die Qualität der Ausbildung in einem Nachwuchsleistungszentrum eines Vereins der deutschen Bundesliga wird vom Deutschen Fußball-Bund (DFB), der Deutschen Fußball Liga (DFL), Spielerberatern, Scouts, der Öffentlichkeit und den Medien bisher anhand verschiedener Kriterien bewertet, etwa an den Erfolgen der Nachwuchsmannschaften, der Integration in einen Profikader, an Infrastruktur und Ausstattung. In wirtschaftlich angespannten Zeiten reichen diese Kriterien für die Vereine jedoch nicht, um den Erfolg von Nachwuchsleistungszentren umfassend zu messen. Die vorliegende Studie befasst sich mit der Messung des Outcomes der Nachwuchsleistungszentren: der hervorgebrachten Spielerqualität bewertet mit Marktpreisen. So wird eine Analyse der Wirksamkeit und Effizienz aus betriebswirtschaftlicher Perspektive erst ermöglicht. Das Ergebnis der Arbeit ist ein Evaluationskonzept für Nachwuchsleistungszentren.
Novel candidate genes underlying extreme trophic specialization in Caribbean pupfishes
Creators: name: McGirr, Joseph A.
name: Agashe, Deepa
name: Martin, Christopher H.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2016

description: The genetic changes responsible for evolutionary transitions from generalist to specialist phenotypes are poorly understood. Here we examine the genetic basis of craniofacial traits enabling novel trophic specialization in a sympatric radiation of Cyprinodon pupfishes endemic to San Salvador Island, Bahamas. This recent radiation consists of a generalist species and two novel specialists: a small-jawed "snail-eater" and a large-jawed "scale-eater." We genotyped 12 million single nucleotide polymorphisms (SNPs) by whole-genome resequencing of 37 individuals of all three species from nine populations and integrated genome-wide divergence scans with association mapping to identify divergent regions containing putatively causal SNPs affecting jaw size-the most rapidly diversifying trait in this radiation. A mere 22 fixed variants accompanied extreme ecological divergence between generalist and scale-eater species. We identified 31 regions (20 kb) containing variants fixed between specialists that were significantly associated with variation in jaw size which contained 11 genes annotated for skeletal system effects and 18 novel candidate genes never previously associated with craniofacial phenotypes. Six of these 31 regions showed robust signs of hard selective sweeps after accounting for demographic history. Our data are consistent with predictions based on quantitative genetic models of adaptation, suggesting that the effect sizes of regions influencing jaw phenotypes are positively correlated with distance between fitness peaks on a complex adaptive landscape.
The Effects of Clioquinol, Zinc and Copper on the Abundance and Function of Breast Cancer Resistance Protein in Human Brain Microvascular Endothelial Cells
Creators: name: YAP, CHRISTOPHER YUNG PHING
Publisher: name: Monash University
Year: 2021
Subjects: Biochemistry and cell biology not elsewhere classified

description: This thesis examined the effects of clioquinol, zinc and copper on the function and expression of breast cancer resistance protein (BCRP) in human brain microvascular endothelial cells. The findings resulting from this research are the first to demonstrate the ability of BCRP abundance and function to be regulated by modulating cytosolic metal concentrations which may be exploited to influence the CNS passage of therapeutics and endogenous substrates.
Phenoscape: Identifying Candidate Genes for Evolutionary Phenotypes
Creators: name: Lapp, Hilmar
name: Dunham, Rex A.
name: Edmunds, Richard C.
name: Dahdul, Wasila M.
name: Westerfield, Monte
name: Lundberg, John G.
name: Vision, Todd J.
name: Su, Baofeng
name: Mabee, Paula M.
name: Eames, B. Frank
name: Balhoff, James P.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2016

description: Phenotypes resulting from mutations in genetic model organisms can help reveal candidate genes for evolutionarily important phenotypic changes in related taxa. Although testing candidate gene hypotheses experimentally in nonmodel organisms is typically difficult, ontology-driven information systems can help generate testable hypotheses about developmental processes in experimentally tractable organisms. Here, we tested candidate gene hypotheses suggested by expert use of the Phenoscape Knowledgebase, specifically looking for genes that are candidates responsible for evolutionarily interesting phenotypes in the ostariophysan fishes that bear resemblance to mutant phenotypes in zebrafish. For this, we searched ZFIN for genetic perturbations that result in either loss of basihyal element or loss of scales phenotypes, because these are the ancestral phenotypes observed in catfishes (Siluriformes). We tested the identified candidate genes by examining their endogenous expression patterns in the channel catfish, Ictalurus punctatus. The experimental results were consistent with the hypotheses that these features evolved through disruption in developmental pathways at, or upstream of, brpf1 and eda/edar for the ancestral losses of basihyal element and scales, respectively. These results demonstrate that ontological annotations of the phenotypic effects of genetic alterations in model organisms, when aggregated within a knowledgebase, can be used effectively to generate testable, and useful, hypotheses about evolutionary changes in morphology.
Mild bounds on bigravity from primordial gravitational waves
Creators: name: Fasiello, Matteo
name: Ribeiro, Raquel H.
Publisher: name: arXiv
Year: 2015
Subjects: subject: Cosmology and Nongalactic Astrophysics (astro-ph.CO)
subject: General Relativity and Quantum Cosmology (gr-qc)
subject: High Energy Physics - Phenomenology (hep-ph)
subject: High Energy Physics - Theory (hep-th)
subject: FOS: Physical sciences

description: If the amplitude of primordial gravitational waves is measured in the near-future, what could it tell us about bigravity? To address this question, we study massive bigravity theories by focusing on a region in parameter space which is safe from known instabilities. Similarly to investigations on late time constraints, we implicitly assume there is a successful implementation of the Vainshtein mechanism which guarantees that standard cosmological evolution is largely unaffected. We find that viable bigravity models are subject to far less stringent constraints than massive gravity, where there is only one set of (massive) tensor modes. In principle sensitive to the effective graviton mass at the time of recombination, we find that in our setup the primordial tensor spectrum is more responsive to the dynamics of the massless tensor sector rather than its massive counterpart. We further show there are intriguing windows in the parameter space of the theory which could potentially induce distinctive signatures in the B-modes spectrum.
description: 20 pages, 1 table, 1 figure
ปัจจัยที่ส่งผลกับความตั้งใจซื้อบ้านของกลุ่มคนที่มีครอบครัว
Creators: name: พลเอก สังฆคุณ
Publisher: name: คณะบริหารธุรกิจ เศรษฐศาสตร์และการสื่อสาร มหาวิทยาลัยนเรศวร
Year: 2560
Subjects: ทฤษฎีพฤติกรรมตามแบบแผน
ทัศนคติต่อการซื้อบ้าน
อิทธิพลของกลุ่มอ้างอิง
การรับรู้ความสามารถในการควบคุมพฤติกรร
ความตั้งใจซื้อบ้าน
The theory of planned behavior
Attitude towards behavior
Group Reference Influence
Perceived behavioral control
Home purchase intention

description: วารสารบริหารธุรกิจ เศรษฐศาสตร์และการสื่อสาร, 12, 2, 145-160
Accelerating MCMC with active subspaces
Creators: name: Constantine, Paul G.
name: Kent, Carson
name: Bui-Thanh, Tan
Publisher: name: arXiv
Year: 2015
Subjects: subject: Numerical Analysis (math.NA)
subject: Computation (stat.CO)
subject: FOS: Mathematics
subject: FOS: Computer and information sciences

description: The Markov chain Monte Carlo (MCMC) method is the computational workhorse for Bayesian inverse problems. However, MCMC struggles in high-dimensional parameter spaces, since its iterates must sequentially explore the high-dimensional space. This struggle is compounded in physical applications when the nonlinear forward model is computationally expensive. One approach to accelerate MCMC is to reduce the dimension of the state space. Active subspaces are part of an emerging set of tools for subspace-based dimension reduction. An active subspace in a given inverse problem indicates a separation between a low-dimensional subspace that is informed by the data and its orthogonal complement that is constrained by the prior. With this information, one can run the sequential MCMC on the active variables while sampling independently according to the prior on the inactive variables. However, this approach to increase efficiency may introduce bias. We provide a bound on the Hellinger distance between the true posterior and its active subspace- exploiting approximation. And we demonstrate the active subspace-accelerated MCMC on two computational examples: (i) a two-dimensional parameter space with a quadratic forward model and one-dimensional active subspace and (ii) a 100-dimensional parameter space with a PDE-based forward model and a two-dimensional active subspace.
title: Chile – Auf dem Weg zu einer neuen Demokratie?
title: Chile ¿rumbo a una nueva democracia?
titleType: AlternativeTitle
Creators: name: (:Unkn) Unknown
Publisher: name: Universität Bremen
Year: 2023
Subjects: subject: Chile
subject: chilenische Verfassungsdebatte
subject: Geschichte Chiles
subject: Extraktivismus
subject: chilenisches Arbeitsrecht
subject: chilenische Frauenbewegung
subject: chilenische Arbeiterbewegung
subject: Verfassungskonvent
subject: Wahrheitskommission
subject: Pinochet
subject: Manifiestos der Historiker
subject: Massaker Iquique
subject: 980

description: Proteste von Schüler:innen gegen eine Erhöhung der Metropreise um 30 Pesos führten in Chile im Oktober 2019 zu einer sozialen Revolte (estallido social). Aus der Kritik an 30 Pesos wurde eine Kritik an der Politik der letzten 30 Jahre nach dem Ende der Pinochet-Diktatur. Die Revolte richtete sich gegen die massive soziale Ungleichheit und mündete in die Forderung nach einer neuen Verfassung. In der bis heute geltenden Verfassung von 1980 ist das neoliberale Wirtschaftsmodell verankert. Der Band zu der 18. szenischen Lesung der Reihe „Aus den Akten auf die Bühne“ enthält Beiträge und Quellen zu der Geschichte Chiles im 20./21. Jahrhundert. Themen sind zum Beispiel das Massaker an der Schule Santa Maria de Iquique (1907), Rohstoffe – Chiles offene Ader, Erinnerung als gesellschaftliche Intervention, Einfluss von Frauenbewegungen, Plurinationalität, Geschichte des Arbeitsrechts, Wahrheitskommissionen sowie Verfassungsgeschichte und Verfassungsdebatte.
Delivery of pDNA using lipid nanoparticles: impact of lipid composition, molar ratio, and route of administration
Creators: name: Algarni, Azizah
Publisher: name: Monash University
Year: 2021
Subjects: Gene and molecular therapy
Nanobiotechnology
Gene expression (incl. microarray and other genome-wide approaches)
Nanomaterials
Nanomedicine

description: Lipid nanoparticle (LNP) is a promising non-viral nanocarrier for in-vivo delivery of nucleic acids into cells. However, LNPs have been extensively optimised for siRNA delivery and it remains unclear whether such optimum LNP formulations can also deliver pDNA effectively. In this thesis, I aimed to elucidate the influence of lipid composition and physicochemical properties of LNPs and the effect of administration routes on pDNA delivery efficiency. The outcomes revealed important new knowledge that can serve as a guideline for the development of next-generation LNPs for highly efficient pDNA delivery.
Supplemental table S1
Creators: name: Martínez Serrano, Antonio
name: Radaelli, Régis
name: Freitas, Tomás T.
name: Alcaraz, Pedro E.
name: Freitas, Sandro R.
Publisher: name: figshare
Year: 2023
Subjects: classificationCode: 420702
subject: Exercise physiology
classificationCode: 420701
subject: Biomechanics
classificationCode: 420703
subject: Motor control

description: Supplemental table S1
R2d2 Drives Selfish Sweeps in the House Mouse
Creators: name: Searle, Jeremy B.
name: Didion, John P.
name: Pomp, Daniel
name: Solano, Emanuela
name: López-Fuster, María J.
name: Ventura, Jacint
name: Ramalhinho, Maria Da Graça Morgado
name: Churchill, Gary A.
name: Morgan, Andrew P.
name: McMullan, Rachel C.
name: Giménez, Mabel D.
name: Ching, Yung-Hao
name: Garland, Theodore
name: Castiglia, Riccardo
name: Bult, Carol J.
name: Gündüz, Islam
name: Bell, Timothy A.
name: Grize, Sofia A.
name: Chesler, Elissa J.
name: Lindholm, Anna K.
name: Weinstock, George M.
name: Gatti, Daniel M.
name: Hauffe, Heidi C.
name: Förster, Daniel W.
name: French, John E.
name: Da Luz Mathias, Maria
name: Holmes, Andrew
name: Giagia-Athanasopoulou, Eva B.
name: Mitsainas, George
name: De Solorzano, Lydia Ortiz
name: Rehermann, Barbara
name: Rosshart, Stephan P.
name: De Villena, Fernando Pardo-Manuel
name: Chunco, Amanda J.
name: Britton-Davidian, Janice
name: Threadgill, David W.
name: Svenson, Karen L.
name: Holt, James M.
name: Gabriel, Sofia I.
name: Shiao, Meng-Shin
name: Crowley, James J.
name: Campbell, Karl J.
name: Hua, Kunjie
name: McMillan, Leonard
name: Jolley, Wesley J.
name: Herman, Jeremy S.
name: Yadgary, Liran
name: Thomas-Laemont, Patricia
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2016

description: A selective sweep is the result of strong positive selection driving newly occurring or standing genetic variants to fixation, and can dramatically alter the pattern and distribution of allelic diversity in a population. Population-level sequencing data have enabled discoveries of selective sweeps associated with genes involved in recent adaptations in many species. In contrast, much debate but little evidence addresses whether “selfish” genes are capable of fixation—thereby leaving signatures identical to classical selective sweeps—despite being neutral or deleterious to organismal fitness. We previously described R2d2, a large copy-number variant that causes nonrandom segregation of mouse Chromosome 2 in females due to meiotic drive. Here we show population-genetic data consistent with a selfish sweep driven by alleles of R2d2 with high copy number (R2d2HC) in natural populations. We replicate this finding in multiple closed breeding populations from six outbred backgrounds segregating for R2d2 alleles. We find that R2d2HC rapidly increases in frequency, and in most cases becomes fixed in significantly fewer generations than can be explained by genetic drift. R2d2HC is also associated with significantly reduced litter sizes in heterozygous mothers, making it a true selfish allele. Our data provide direct evidence of populations actively undergoing selfish sweeps, and demonstrate that meiotic drive can rapidly alter the genomic landscape in favor of mutations with neutral or even negative effects on overall Darwinian fitness. Further study will reveal the incidence of selfish sweeps, and will elucidate the relative contributions of selfish genes, adaptation and genetic drift to evolution.
An image cytometer based on angular spatial frequency processing and its validation for rapid detection and quantification of waterborne microorganisms
Creators: name: Pérez, Juan Miguel
name: Jofre, Marc
name: Martínez, Pedro
name: Pruneri, Valerio
Publisher: name: arXiv
Year: 2015
Subjects: subject: Optics (physics.optics)
subject: FOS: Physical sciences

description: We introduce a new image cytometer design for detection of very small particulate and demonstrate its capability in water analysis. The device is a compact microscope composed of off the shelf components, such as a light emitting diode (LED) source, a complementary metal oxide semiconductor (CMOS) image sensor, and a specific combination of optical lenses that allow, through an appropriate software, Fourier transform processing of the sample volume. Waterborne microorganisms, such as Escherichia coli (E. coli), Legionella pneumophila (L. pneumophila) and Phytoplankton, are detected by interrogating the volume sample either in a fluorescent or label-free mode, i.e. with or without fluorescein isothiocyanate (FITC) molecules attached to the micro-organisms, respectively. We achieve a sensitivity of 50 cells/ml, which can be further increased to 0.2 cells/ml by preconcentrating an initial sample volume of 500 ml with an adhoc fluidic system. We also prove the capability of the proposed image cytometer of differentiating microbiological populations by size with a resolution of 3 um and of operating in real contaminated water.
Context dependent cytokine secretion by stem cell derived microglia in vitro
Creators: name: TERESA HELENA VANDEKOLK
Publisher: name: Monash University
Year: 2020
Subjects: Stem Cells
Neuroscience

description: This thesis demonstrated that inflammation by brain immune called microglia, is context dependent. Previous studies have used microglia derived from human embryonic stem cells to model Alzheimer's disease, but have not investigated whether the responses are uniform across models. This thesis used stem cell-derived microglia, under multiple culture conditions, to demonstrate that differences in inflammation is due to environmental context. It was clearly demonstrated that microglia grown with neurons were more inflammatory than microglia grown alone. Additionally, this thesis shows the inflammation profile is altered by how inflammation is stimulated. This is the first study to demonstrate clear context dependent differences in stem cell-derived microglia inflammation models. This indicates future works should take care to choose appropriate culturing conditions and stimulus choices for modelling diseases of the brain for optimal experimental outcomes.
Increased Affinity for RNA Targets Evolved Early in Animal and Plant Dicer Lineages through Different Structural Mechanisms
Creators: name: Jia, Haiyan
name: Rolland, James
name: Kolaczkowski, Bryan
name: Kolaczkowski, Oralia
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2017

description: Understanding the structural basis for evolutionary changes in protein function is central to molecular evolutionary biology and can help determine the extent to which functional convergence occurs through similar or different structural mechanisms. Here, we combine ancestral sequence reconstruction with functional characterization and structural modeling to directly examine the evolution of sequence-structure-function across the early differentiation of animal and plant Dicer/DCL proteins, which perform the first molecular step in RNA interference by identifying target RNAs and processing them into short interfering products. We found that ancestral Dicer/DCL proteins evolved similar increases in RNA target affinities as they diverged independently in animal and plant lineages. In both cases, increases in RNA target affinities were associated with sequence changes that anchored the RNA's 5'phosphate, but the structural bases for 5'phosphate recognition were different in animal versus plant lineages. These results highlight how molecular-functional evolutionary convergence can derive from the evolution of unique protein structures implementing similar biochemical mechanisms.
Highly Retrievable Spinwave-Photon Entanglement Source
Creators: name: Yang, Sheng-Jun
name: Wang, Xu-Jie
name: Li, Jun
name: Rui, Jun
name: Bao, Xiao-Hui
name: Pan, Jian-Wei
Publisher: name: arXiv
Year: 2015
Subjects: subject: Quantum Physics (quant-ph)
subject: FOS: Physical sciences

description: Entanglement between a single photon and a quantum memory forms the building blocks for quantum repeater and quantum network. Previous entanglement sources are typically with low retrieval efficiency, which limits future larger-scale applications. Here, we report a source of highly retrievable spinwave-photon entanglement. Polarization entanglement is created through interaction of a single photon with ensemble of atoms inside a low-finesse ring cavity. The cavity is engineered to be resonant for dual spinwave modes, which thus enables efficient retrieval of the spinwave qubit. An intrinsic retrieval efficiency up to 76(4)% has been observed. Such a highly retrievable atom-photon entanglement source will be very useful in future larger-scale quantum repeater and quantum network applications.
description: 5 pages, 3 figures
Peptide toxins: structure, stability and engineering
Creators: name: STEPHEN BERNARD DRANE
Publisher: name: Monash University
Year: 2018
Subjects: subject: 30406 Proteins and Peptides
subject: FOS: Chemical sciences
subject: Biochemistry

description: This thesis is an investigation of peptide toxins from animal venom, aimed to determine the utility of the scorpion toxin HsTX1, as well as the conotoxins H_Vc7.1, H_Vc7.2 and contryphan-Vc2. Part of the work includes efforts undertaken to validate the scorpion toxin HsTX1 from the species Heterometrus spinifer as a scaffold structure for peptide engineering by molecular grafting. In addition, the production and biochemical characterisation of recently discovered conotoxins found in the venom duct of the venomous marine snail Conus victoriae are described.
Interdependence, Reflexivity, Fidelity, Impedance Matching, and the Evolution of Genetic Coding
Creators: name: Carter, Charles W.
name: Wills, Peter R.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2018

description: Genetic coding is generally thought to have required ribozymes whose functions were taken over by polypeptide aminoacyl-tRNA synthetases (aaRS). Two discoveries about aaRS and their interactions with tRNA substrates now furnish a unifying rationale for the opposite conclusion: that the key processes of the Central Dogma of molecular biology emerged simultaneously and naturally from simple origins in a peptide•RNA partnership, eliminating the epistemological utility of a prior RNA world. First, the two aaRS classes likely arose from opposite strands of the same ancestral gene, implying a simple genetic alphabet. The resulting inversion symmetries in aaRS structural biology would have stabilized the initial and subsequent differentiation of coding specificities, rapidly promoting diversity in the proteome. Second, amino acid physical chemistry maps onto tRNA identity elements, establishing reflexive, nanoenvironmental sensing in protein aaRS. Bootstrapping of increasingly detailed coding is thus intrinsic to polypeptide aaRS, but impossible in an RNA world. These notions underline the following concepts that contradict gradual replacement of ribozymal aaRS by polypeptide aaRS: (i) aaRS enzymes must be interdependent; (ii) reflexivity intrinsic to polypeptide aaRS production dynamics promotes bootstrapping; (iii) takeover of RNA-catalyzed aminoacylation by enzymes will necessarily degrade specificity; (iv) the Central Dogma's emergence is most probable when replication and translation error rates remain comparable. These characteristics are necessary and sufficient for the essentially de novo emergence of a coupled gene-replicase-translatase system of genetic coding that would have continuously preserved the functional meaning of genetically encoded protein genes whose phylogenetic relationships match those observed today.
Das Konzept der Sinus-Milieus für eine zielgruppen-spezifische Strategieentwicklung in der kommunalen Wärmeplanung am Beispiel Bremens
Creators: name: Schwarz, Timmy
name: Kötterheinrich, Kristina
name: Knies, Jürgen
Publisher: name: Hochschule Bremen
Year: 2023
Subjects: subject: Sinus-Milieu
subject: zielgruppenspezifisch
subject: Wärmeplanung
subject: Wärmewende
subject: GIS
subject: Quartier
subject: Energiewende
subject: 300

description: Die Dekarbonisierung der Energieversorgung im Gebäudesektor wird zu tiefgreifenden Veränderungen in einem Bereich führen, welcher den Alltag der Menschen direkt betrifft: dem Wohnen. Sinus-Milieu-Daten können helfen, Quartiere und ihre Bewohnerschaft besser einzuschätzen. So können politische Maßnahmen spezifischer eingesetzt werden. Die vorliegende Arbeit untersucht, wie sich die spezifischen Sinus-Milieus in Bezug auf die Transformation im Gebäudesektor bei Investitions- und Konsumentscheidungen unterschiedlich aufstellen und zeigt am Beispiel der Stadt Bremen, wie sich Sinus-Milieus geografisch clustern. Zudem werden Vorschläge gemacht, wie diese Daten für politische Maßnahmen und weitere Forschung genutzt werden können.
Trafficking and compartmentalized signaling of the delta-opioid receptor
Creators: name: Tiah, Lih En
Publisher: name: Monash University
Year: 2017
Subjects: Uncategorized

description: Opioid receptors are class A G protein coupled receptors (GPCRs). The delta-opioid receptor (DOPr) in particular is a key therapeutic target for chronic pain and gastrointestinal disorders. Like other opioid receptors, drugs targeting DOPr are effective analgesics but their use is limited due to their addictive liabilities they possess. Recent studies suggest that DOPr internalization may contribute towards the development of tolerance and analgesia. Once considered merely as cell surface sensors, more recent studies have revealed the importance of GPCR signaling from within endosomes. Moreover, the temporal and spatial segregation of signals generated by agonist stimulated GPCR trafficking can give rise to a range of discrete cellular responses following activation of the same second messenger systems. This is referred to as compartmentalized signaling. The study of how differential receptor trafficking in response to various ligands underlies potential differences in compartmentalized signaling profiles may therefore be key to selectively promote physiologically beneficial signaling without the deleterious side effects. This thesis reveals that DOPr endocytosis is critically important for opioid signaling in subcellular compartments, which may account for the differential effects of DOPr agonists on analgesia and tolerance. Our findings reveal differences in the recruitment of regulatory proteins to DOPr following stimulation with agonists that induce varying degrees of DOPr internalization, which dictates the pathway through which DOPr traffics. This thesis also illustrates distinct signaling responses as a consequence of differential agonist stimulated DOPr trafficking thus highlighting the importance of DOPr internalization for the activation and inhibition of specific signaling cascades. Collectively, the present findings demonstrate a link between DOPr trafficking and compartmentalized signaling which may underlie the beneficial therapeutic effects of weakly internalizing DOPr agonists.
title: Exploring surface electromyography (EMG) as a feedback variable for the human-in-the-loop optimization of lower limb wearable robotics
Creators: name: Grimmer, Martin
name: Zeiss, Julian
name: Weigand, Florian
name: Zhao, Guoping
Publisher: name: Frontiers Media S.A.
Year: 2022

description: Human-in-the-loop (HITL) optimization with metabolic cost feedback has been proposed to reduce walking effort with wearable robotics. This study investigates if lower limb surface electromyography (EMG) could be an alternative feedback variable to overcome time-intensive metabolic cost based exploration. For application, it should be possible to distinguish conditions with different walking efforts based on the EMG. To obtain such EMG data, a laboratory experiment was designed to elicit changes in the effort by loading and unloading pairs of weights (in total 2, 4, and 8 kg) in three randomized weight sessions for 13 subjects during treadmill walking. EMG of seven lower limb muscles was recorded for both limbs. Mean absolute values of each stride prior to and following weight loading and unloading were used to determine the detection rate (100% if every loading and unloading is detected accordingly) for changing between loaded and unloaded conditions. We assessed the use of multiple consecutive strides and the combination of muscles to improve the detection rate and estimated the related acquisition times of diminishing returns. To conclude on possible limitations of EMG for HITL optimization, EMG drift was evaluated during the Warmup and the experiment. Detection rates highly increased for the combination of multiple consecutive strides and the combination of multiple muscles. EMG drift was largest during Warmup and at the beginning of each weight session. The results suggest using EMG feedback of multiple involved muscles and from at least 10 consecutive strides (5.5 s) to benefit from the increases in detection rate in HITL optimization. In combination with up to 20 excluded acclimatization strides, after changing the assistance condition, we advise exploring about 16.5 s of walking to obtain reliable EMG-based feedback. To minimize the negative impact of EMG drift on the detection rate, at least 6 min of Warmup should be performed and breaks during the optimization should be avoided. Future studies should investigate additional feedback variables based on EMG, methods to reduce their variability and drift, and should apply the outcomes in HITL optimization with lower limb wearable robots.
The Rate of Evolution of Postmating-Prezygotic Reproductive Isolation in Drosophila
Creators: name: McGirr, Joseph A.
name: Turissini, David A.
name: Matute, Daniel R.
name: David, Jean R.
name: Patel, Sonali S.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2018

description: Reproductive isolation is an intrinsic aspect of species formation. For that reason, the identification of the precise isolating traits, and the rates at which they evolve, is crucial to understanding how species originate and persist. Previous work has measured the rates of evolution of prezygotic and postzygotic barriers to gene flow, yet no systematic analysis has studied the rates of evolution of postmating-prezygotic (PMPZ) barriers. We measured the magnitude of two barriers to gene flow that act after mating occurs but before fertilization. We also measured the magnitude of a premating barrier (female mating rate in nonchoice experiments) and two postzygotic barriers (hybrid inviability and hybrid sterility) for all pairwise crosses of all nine known extant species within the melanogaster subgroup. Our results indicate that PMPZ isolation evolves faster than hybrid inviability but slower than premating isolation. Next, we partition postzygotic isolation into different components and find that, as expected, hybrid sterility evolves faster than hybrid inviability. These results lend support for the hypothesis that, in Drosophila, reproductive isolation mechanisms (RIMs) that act early in reproduction (or in development) tend to evolve faster than those that act later in the reproductive cycle. Finally, we tested whether there was evidence for reinforcing selection at any RIM. We found no evidence for generalized evolution of reproductive isolation via reinforcement which indicates that there is no pervasive evidence of this evolutionary process. Our results indicate that PMPZ RIMs might have important evolutionary consequences in initiating speciation and in the persistence of new species.
rAAV-compatible MiniPromoters for restricted expression in the brain and eye
Creators: name: Goldowitz, Daniel
name: Borretta, Lisa J.
name: Zhou, Michelle
name: Laprise, Stéphanie
name: Holt, Robert A.
name: Yang, George S.
name: Lengyell, Tess C.
name: De Leeuw, Charles N.
name: Wasserman, Wyeth W.
name: Swanson-Newman, Magdalena I.
name: Chopra, Vikramjit
name: D'Souza, Cletus A.
name: Portales-Casamar, Elodie
name: Asokan, Aravind
name: Kaspieva, Olga
name: Chou, Alice Y.
name: Korecki, Andrea J.
name: Wong, Kaelan
name: Berry, Garrett E.
name: Jones, Steven J. M.
name: Hickmott, Jack W.
name: Bonaguro, Russell J.
name: Lam, Siu Ling
name: Simpson, Elizabeth M.
name: McInerny, Simone C.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2016

description: Abstract Background Small promoters that recapitulate endogenous gene expression patterns are important for basic, preclinical, and now clinical research. Recently, there has been a promising revival of gene therapy for diseases with unmet therapeutic needs. To date, most gene therapies have used viral-based ubiquitous promoters–however, promoters that restrict expression to target cells will minimize off-target side effects, broaden the palette of deliverable therapeutics, and thereby improve safety and efficacy. Here, we take steps towards filling the need for such promoters by developing a high-throughput pipeline that goes from genome-based bioinformatic design to rapid testing in vivo. Methods For much of this work, therapeutically interesting Pleiades MiniPromoters (MiniPs; ~4 kb human DNA regulatory elements), previously tested in knock-in mice, were “cut down” to ~2.5 kb and tested in recombinant adeno-associated virus (rAAV), the virus of choice for gene therapy of the central nervous system. To evaluate our methods, we generated 29 experimental rAAV2/9 viruses carrying 19 different MiniPs, which were injected intravenously into neonatal mice to allow broad unbiased distribution, and characterized in neural tissues by X-gal immunohistochemistry for icre, or immunofluorescent detection of GFP. Results The data showed that 16 of the 19 (84 %) MiniPs recapitulated the expression pattern of their design source. This included expression of: Ple67 in brain raphe nuclei; Ple155 in Purkinje cells of the cerebellum, and retinal bipolar ON cells; Ple261 in endothelial cells of brain blood vessels; and Ple264 in retinal Müller glia. Conclusions Overall, the methodology and MiniPs presented here represent important advances for basic and preclinical research, and may enable a paradigm shift in gene therapy.