Additional file 8 of Mis-localization of endogenous TDP-43 leads to ALS-like early-stage metabolic dysfunction and progressive motor deficits
Creators: name: Hu, Yiying
name: Hruscha, Alexander
name: Pan, Chenchen
name: Schifferer, Martina
name: Schmidt, Michael K.
name: Nuscher, Brigitte
name: Giera, Martin
name: Kostidis, Sarantos
name: Burhan, Özge
name: van Bebber, Frauke
name: Edbauer, Dieter
name: Arzberger, Thomas
name: Haass, Christian
name: Schmid, Bettina
Publisher: name: figshare
Year: 2024
Subjects: subject: Biochemistry
subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Neuroscience
subject: Physiology
subject: Pharmacology
subject: Virology

description: Supplementary Material 8: SFigure 7. Detailed CytoTDP behaviour data. (A-B) Quantifications of small-bouts and large-bouts distance pencentage for CytoTDP (red dots) and its siblings [Siblings (black arrow heads): tardbp ΔNLS/ + ; tardbpl -/-, Control (black dots): tardbp + / + ; tardbpl -/-] at 5 dpf and 8 dpf. Kruskal–Wallis test was used for 3 group comparisons and Mann–Whitney test was used for 2 group comparisons. Error bars indicates ± interquartile range. Control n = 21, Siblings n = 50, CytoTDP n = 23. (A) Small-bouts distance pencentage [5 dpf (**p = 0.0015, Control vs Siblings p &gt; 0.9999, Control vs CytoTDP * p = 0.0324, Siblings vs CytoTDP ** p = 0.0012); 8 dpf (****p &lt; 0.0001, Control vs Siblings p &gt; 0.9999, Control vs CytoTDP ** p = 0.0023, Siblings vs CytoTDP **** p &lt; 0.0001)]; (B) Large-bouts distance percentage for CytoTDP and its siblings at 5 dpf and 8 dpf. [5 dpf (**p = 0.0015, Control vs Siblings p &gt; 0.99, Control vs CytoTDP * p = 0.0375, Siblings vs CytoTDP ** p = 0.0011); 8 dpf (****p &lt; 0.0001, Control vs Siblings p &gt; 0.9999, Control vs CytoTDP ** p = 0.0023, Siblings vs CytoTDP **** p &lt; 0.0001)]; (C) Stacked bar graph showing mean distribution of small-bouts distance (white bar) and large-bouts distance (red bar) for CytoTDP and its siblings at 5 dpf and 8 dpf. (D-E) Quantifications of small-bouts and large-bouts duration percentage for CytoTDP and its siblings at 5 dpf and 8 dpf. Kruskal–Wallis test was used for 3 group comparisons and Mann–Whitney test was used for 2 group comparisons. Error bars indicates ± interquartile range. Control n = 21, Siblings n = 50, CytoTDP n = 23. (D) Small-bouts duration pencentage [5 dpf (**p = 0.0015, Control vs Siblings p &gt; 0.99, Control vs CytoTDP * p = 0.0324, Siblings vs CytoTDP ** p = 0.0012); 8 dpf (****p &lt; 0.0001, Control vs Siblings p &gt; 0.9999, Control vs CytoTDP ** p = 0.0023, Siblings vs CytoTDP **** p &lt; 0.0001)] (E) Large-bouts duration percentage for CytoTDP and its siblings at 5 dpf and 8 dpf. [5 dpf (**p = 0.0015, Control vs Siblings p &gt; 0.9999, Control vs CytoTDP * p = 0.0375, Siblings vs CytoTDP ** p = 0.0011); 8 dpf (****p &lt; 0.0001, Control vs Siblings p &gt; 0.9999, Control vs CytoTDP ** p = 0.0023, Siblings vs CytoTDP **** p &lt; 0.0001)] (F) Stacked bar graph showing mean distribution of small-bouts (white bar) duration, large-bouts (red bar) duration and still duration (grey bar) for CytoTDP and its siblings at 5 dpf and 8 dpf.