cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
A qualitative investigation of factors influencing the dietary intakes of professional Australian football players
Creators: name: Jenner, Sarah
name: Belski, Regina
name: Devlin, Brooke
name: A Coutts
name: T Kempton
name: Forsyth, Adrienne
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: (1) Background: Many professional Australian Football (AF) players do not meet recom-mended sports nutrition guidelines despite having access to nutrition advice. There are a range of factors that can influence players′ ability to meet their nutrition goals and awareness of the barriers players face is essential to ensure that dietary advice translates into practice. Therefore, this qualitative research study aimed to explore the factors influencing AF players’ dietary intakes and food choice. (2) Methods: Semi-structured interviews were conducted with twelve professional male AF players. (3) Results: Less experienced players restricted their carbohydrate intake to meet body composition goals, particularly during preseason and surrounding body composition assessment. During the competition season players had a greater focus on performance and placed more emphasis on carbohydrate intake in the lead up to matches. Players felt nutrition goals were easier to achieve when dietary choices were supported by their families and peers. One-on-one consultations pro-vided by a sports dietitian were players′ preferred mode of nutrition intervention. Individualized nutrition advice is required for less experienced AF players who may be vulnerable to unsustainable dietary habits. Experienced AF players can support junior teammates by promoting positive team culture related to body composition, nutrition and performance.
Is there an association between breast hypoplasia and breastfeeding outcomes? A systematic review
Creators: name: Kam, Renee
name: Amir, Lisa
name: Cullinane, Meabh
Publisher: name: La Trobe
Year: 2022
Subjects: classificationCode: 32
subject: Biomedical and clinical sciences
classificationCode: 3210
subject: Nutrition and dietetics
classificationCode: 3213
subject: Paediatrics
classificationCode: 3215
subject: Reproductive medicine

description: No description supplied
title: Letter: C. Harrison Mann, Jr. to Mills E. Godwin, Jr., December 12, 1965
Creators: name: Mann, Charles Harrison, Jr., 1908-1977
Publisher: name: George Mason University
Year: 2005
Subjects: subject: Godwin, Mills E. (Mills Edwin), 1914-
subject: Virginia College Building Authority
subject: Legislation
subject: Finance

description: Letter: typescript unsigned, 11" x 8.5" (29.7 cm x 21.6 cm)
description: Letter from C. Harrison Mann, Jr. to Mills E. Godwin, Jr., Virginia Lieutenant Governor, regarding the redrafting of legislation to create the Virginia College Building Authority and then discusses how it will be financed. C. Harrison Mann, Jr. Collection 1.16
Evaluating health service outcomes of public involvement in health service design in high-income countries: a systematic review
Creators: name: Lloyd, Nicola
name: Kenny, Amanda
name: Nerida Hyett
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Background: Internationally, it is expected that health services will involve the public in health service design. Evaluation of public involvement has typically focused on the process and experiences for participants. Less is known about outcomes for health services. The aim of this systematic review was to a) identify and synthesise what is known about health service outcomes of public involvement and b) document how outcomes were evaluated. Methods: Searches were undertaken in MEDLINE, EMBASE, The Cochrane Library, PsycINFO, Web of Science, and CINAHL for studies that reported health service outcomes from public involvement in health service design. The review was limited to high-income countries and studies in English. Study quality was assessed using the Mixed Methods Appraisal Tool and critical appraisal guidelines for assessing the quality and impact of user involvement in health research. Content analysis was used to determine the outcomes of public involvement in health service design and how outcomes were evaluated. Results: A total of 93 articles were included. The majority were published in the last 5 years, were qualitative, and were located in the United Kingdom. A range of health service outcomes (discrete products, improvements to health services and system/policy level changes) were reported at various levels (service level, across services, and across organisations). However, evaluations of outcomes were reported in less than half of studies. In studies where outcomes were evaluated, a range of methods were used; most frequent were mixed methods. The quality of study design and reporting was inconsistent. Conclusion: When reporting public involvement in health service design authors outline a range of outcomes for health services, but it is challenging to determine the extent of outcomes due to inadequate descriptions of study design and poor reporting. There is an urgent need for evaluations, including longitudinal study designs and cost-benefit analyses, to fully understand outcomes from public involvement in health service design.
Proteome characterisation of extracellular vesicles isolated from heart
Creators: name: Claridge, Bethany
name: Rai, Alin
name: Fang, H
name: Matsumoto, A
name: Luo, J
name: McMullen, Julie
name: Greening, David
Publisher: name: La Trobe
Year: 2023
Subjects: classificationCode: 31
subject: Biological sciences
subject: FOS: Biological sciences
classificationCode: 3101
subject: Biochemistry and cell biology
classificationCode: 32
subject: Biomedical and clinical sciences
classificationCode: 3208
subject: Medical physiology

description: Cardiac intercellular communication is critical for heart function and often dysregulated in cardiovascular diseases. While cardiac extracellular vesicles (cEVs) are emerging mediators of signalling, their isolation remains a technical challenge hindering our understanding of cEV protein composition. Here, we utilised Langendorff-collagenase-based enzymatic perfusion and differential centrifugation to isolate cEVs from mouse heart (yield 3–6 μg/heart). cEVs are ∼200 nm, express classical EV markers (Cd63/81/9+, Tsg101+, Pdcd6ip/Alix+), and are depleted of blood (Alb/Fga/Hba) and cardiac damage markers (Mb, Tnnt2, Ldhb). Comparison with mechanically-derived EVs revealed greater detection of EV markers and decreased cardiac damage contaminants. Mass spectrometry-based proteomic profiling revealed 1721 proteins in cEVs, implicated in proteasomal and autophagic proteostasis, glycolysis, and fatty acid metabolism; essential functions often disrupted in cardiac pathologies. There was striking enrichment of 942 proteins in cEVs compared to mouse heart tissue - implicated in EV biogenesis, antioxidant activity, and lipid transport, suggesting active cargo selection and specialised function. Interestingly, cEVs contain marker proteins for cardiomyocytes, cardiac progenitors, B-cells, T-cells, macrophages, smooth muscle cells, endothelial cells, and cardiac fibroblasts, suggesting diverse cellular origin. We present a method of cEV isolation and provide insight into potential functions, enabling future studies into EV roles in cardiac physiology and disease.
Proteome characterisation of extracellular vesicles isolated from heart
Creators: name: Claridge, Bethany
name: Rai, A
name: Fang, H
name: Matsumoto, A
name: Luo, J
name: McMullen, Julie
name: Greening, David
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Cardiac intercellular communication is critical for heart function and often dysregulated in cardiovascular diseases. While cardiac extracellular vesicles (cEVs) are emerging mediators of signalling, their isolation remains a technical challenge hindering our understanding of cEV protein composition. Here, we utilised Langendorff-collagenase-based enzymatic perfusion and differential centrifugation to isolate cEVs from mouse heart (yield 3–6 μg/heart). cEVs are ∼200 nm, express classical EV markers (Cd63/81/9+, Tsg101+, Pdcd6ip/Alix+), and are depleted of blood (Alb/Fga/Hba) and cardiac damage markers (Mb, Tnnt2, Ldhb). Comparison with mechanically-derived EVs revealed greater detection of EV markers and decreased cardiac damage contaminants. Mass spectrometry-based proteomic profiling revealed 1721 proteins in cEVs, implicated in proteasomal and autophagic proteostasis, glycolysis, and fatty acid metabolism; essential functions often disrupted in cardiac pathologies. There was striking enrichment of 942 proteins in cEVs compared to mouse heart tissue - implicated in EV biogenesis, antioxidant activity, and lipid transport, suggesting active cargo selection and specialised function. Interestingly, cEVs contain marker proteins for cardiomyocytes, cardiac progenitors, B-cells, T-cells, macrophages, smooth muscle cells, endothelial cells, and cardiac fibroblasts, suggesting diverse cellular origin. We present a method of cEV isolation and provide insight into potential functions, enabling future studies into EV roles in cardiac physiology and disease.
Condition assessment for concrete sewer pipes using displacement probes: a robotic design case study
Creators: name: Ross, Robert
name: Stumpf, Alexander
name: D Barnett
name: Hall, Richard
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Worldwide, millions of kilometres of sewers are constructed from concrete pipes. Unfortunately, concrete sewers are susceptible to corrosion from biogenic hydrogen sulphide, and, though they may pass visual inspection, their ability to hold together under load may be degraded. This paper presents the design of a teleoperated robot with a protractible probe, that allows an operator to apply a localised load to selected points within a concrete sewer pipe. We report findings from laboratory and field trials of our prototype, with initial results suggesting that this approach has the potential to contribute useful information to sewer maintenance planning.
Recent Advances and Future Perspectives on Microfluidic Mix-and-Jet Sample Delivery Devices
Creators: name: Hejazian, Majid
name: Balaur, Eugeniu
name: Abbey, Brian
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: The integration of the Gas Dynamic Virtual Nozzle (GDVN) and microfluidic technologies has proven to be a promising sample delivery solution for biomolecular imaging studies and has the potential to be transformative for a range of applications in physics, biology, and chemistry. Here, we review the recent advances in the emerging field of microfluidic mix-and-jet sample delivery devices for the study of biomolecular reaction dynamics. First, we introduce the key parameters and dimensionless numbers involved in their design and characterisation. Then we critically review the techniques used to fabricate these integrated devices and discuss their advantages and disadvantages. We then summarise the most common experimental methods used for the characterisation of both the mixing and jetting components. Finally, we discuss future perspectives on the emerging field of microfluidic mix-and-jet sample delivery devices. In summary, this review aims to introduce this exciting new topic to the wider microfluidics community and to help guide future research in the field.
Release of the National Scheme’s Aboriginal and Torres Strait Islander Health and Cultural Safety Strategy 2020-2025; the impacts for podiatry in Australia: a commentary
Creators: name: Gerrard, James
name: Godwin, Shirley
name: Chuter, V
name: Munteanu, Shannon
name: West, M
name: Hawke, F
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Background: Developing since colonisation, Australia’s healthcare system has dismissed an ongoing and successful First Nations health paradigm in place for 60,000 years. From Captain James Cook documenting ‘very old’ First Nations Peoples being ‘far more happier than we Europeans’ and Governor Arthur Phillip naming Manly in admiration of the physical health of Gadigal men of the Eora Nation, to anthropologist Daisy Bates’ observation of First Nations Peoples living ‘into their eighties’ and having a higher life expectancy than Europeans; our healthcare system’s shameful cultural safety deficit has allowed for an Aboriginal and Torres Strait Islander child born in Australia today to expect to live 9 years less than a non-Indigenous child. Disproportionately negative healthcare outcomes including early onset diabetes-related foot disease and high rates of lower limb amputation in Aboriginal and Torres Strait Islander Peoples contribute to this gross inequity. Main body: In 2020, the Australian Health Practitioner Regulation Authority released the National Scheme’s Aboriginal and Torres Strait Islander Health and Cultural Safety Strategy 2020–2025 - empowering all registered health practitioners within Australia to provide health care to Aboriginal and Torres Strait Islander Peoples that is inclusive, respectful and safe, as judged by the recipient of care. This recently released strategy is critically important to the podiatry profession in Australia. As clinicians, researchers and educators we have a collective responsibility to engage with this strategy of cultural safety. This commentary defines cultural safety for podiatry and outlines the components of the strategy in the context of our profession. Discussion considers the impact of the strategy on podiatry. It identifies mechanisms for podiatrists in all settings to facilitate safer practice, thereby advancing healthcare to produce more equitable outcomes. Conclusion: Aboriginal and Torres Strait Islander Peoples access health services more frequently and have better health outcomes where provision of care is culturally safe. By engaging with the National Scheme’s Aboriginal and Torres Strait Islander Health and Cultural Safety Strategy, all registered podiatrists in Australia can contribute to achieving equity in health outcomes for Aboriginal and Torres Strait Islander Peoples.
Comparative evaluation of different molecular methods for DNA extraction from individual Teladorsagia circumcincta nematodes
Creators: name: Sloan, Sarah
name: Jenvey, Caitlin
name: Piedrafita, D
name: Preston, S
name: Stear, Michael
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Background: The purpose of this study was to develop a reliable DNA extraction protocol to use on individual Teladorsagia circumcincta nematode specimens to produce high quality DNA for genome sequencing and phylogenetic analysis. Pooled samples have been critical in providing the groundwork for T. circumcincta genome construction, but there is currently no standard method for extracting high-quality DNA from individual nematodes. 11 extraction kits were compared based on DNA quality, yield, and processing time. Results: 11 extraction protocols were compared, and the concentration and purity of the extracted DNA was quantified. Median DNA concentration among all methods measured on NanoDrop 2000™ ranged between 0.45–11.5 ng/μL, and on Qubit™ ranged between undetectable – 0.962 ng/μL. Median A260/280 ranged between 0.505–3.925, and median A260/230 ranged − 0.005 – 1.545. Larval exsheathment to remove the nematode cuticle negatively impacted DNA concentration and purity. Conclusions: A Schistosoma sp. DNA extraction method was determined as most suitable for individual T. circumcincta nematode specimens due to its resulting DNA concentration, purity, and relatively fast processing time.
Community response to the impact of thunderstorm asthma using smart technology
Creators: name: AlQuran, Ala
name: Batra, Mehak
name: Susanto, Nugroho
name: Holland, Anne
name: Davies, Janet M
name: Erbas, Bircan
name: Lampugnani, Edwin R
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Background The most severe thunderstorm asthma (TA) event occurred in Melbourne on the 21st November 2016 and during this period, daily pollen information was available and accessible on smart devices via an App. An integrated survey within the App allows users to self-report symptoms. Objective To explore patterns of symptom survey results during the period when the TA event occurred. Methods Symptom data from the Melbourne Pollen Count and Forecast App related to asthma history, hay fever symptoms, and medication use was explored. A one-week control period before and after the event was considered. Chi-square tests and logistic regression were used to assess associations between sex, age, symptoms, and medication use. Results Of the 28,655 responses, during the 2016 pollen season, younger (18 to 40 years) males, with no hay fever and no asthma were the most single and regular responders. During the TA event for new users, sex was only significantly associated with hay fever ( p = 0.008) of which 60.2% of females’ responses reported having hay fever, while 43% of males’ responses did not. Those with mild symptoms peaked during the TA event. Conclusions Many individuals completed the survey on the app for the first time during the TA event indicating the potential of digital technologies to be used as indicators of health risk among populations at risk of TA events.
ASPset: An outdoor sports pose video dataset with 3D keypoint annotations
Creators: name: Nibali, Aiden
name: Millward, Joshua
name: He, Zhen
name: Morgan, Stuart
Publisher: name: La Trobe
Year: 2022
Subjects: classificationCode: 4007
subject: Control engineering, mechatronics and robotics
classificationCode: 46
subject: Information and computing sciences
classificationCode: 4603
subject: Computer vision and multimedia computation
classificationCode: 4608
subject: Human-centred computing
classificationCode: 4611
subject: Machine learning

description: No
description supplied
Australian Early Childhood Teachers’ Training in Language and Literacy: A Nation-Wide Review of Pre-Service Course Content
Creators: name: Weadman, Tessa
name: Serry, Tanya
name: Snow, Pamela
Publisher: name: La Trobe
Year: 2024
Subjects: classificationCode: 39
subject: Education
classificationCode: 3901
subject: Curriculum and pedagogy
classificationCode: 3903
subject: Education systems

description: Early childhood teachers are well-positioned to maximise preschoolers’ development in oral language and emergent literacy; both of which are vital predictors of academic success at school. Research investigating their pre-service training in language and emergent literacy remains limited. This issue is addressed in the present study, with the first nation-wide review of the oral language and emergent literacy course content across all 84 Australian early childhood teacher pre-service courses. Qualitative Content Analysis was employed to gain an overview of language and emergent literacy teaching content reported in publicly available course documents. Study findings demonstrated large variation in the oral language and emergent literacy course content reported. The results showed that course content on the structure of language and code-related skills including phonological awareness and concepts of print, do not feature prominently across pre-service course documents. Further, course content on evidence-based strategies to foster children’s oral language and emergent literacy development, such as dialogic book reading, and intentional adult-child interactions were also limited.
Australian Early Childhood Teachers’ Training in Language and Literacy: A Nation-Wide Review of Pre-Service Course Content
Creators: name: Weadman, Tessa
name: Serry, Natanya
name: Snow, Pamela
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Early childhood teachers are well-positioned to maximise preschoolers’ development in oral language and emergent literacy; both of which are vital predictors of academic success at school. Research investigating their pre-service training in language and emergent literacy remains limited. This issue is addressed in the present study, with the first nation-wide review of the oral language and emergent literacy course content across all 84 Australian early childhood teacher pre-service courses. Qualitative Content Analysis was employed to gain an overview of language and emergent literacy teaching content reported in publicly available course documents. Study findings demonstrated large variation in the oral language and emergent literacy course content reported. The results showed that course content on the structure of language and code-related skills including phonological awareness and concepts of print, do not feature prominently across pre-service course documents. Further, course content on evidence-based strategies to foster children’s oral language and emergent literacy development, such as dialogic book reading, and intentional adult-child interactions were also limited.
title: Rewarding Those Hardly Working More Than Those Working Hard: How Misallocating Equitable Team-Based Rewards in Interdependent Tasks Can Create a Barrier to Retaining Higher-Performing Team Members
Creators: name: (:Unkn) Unknown
Publisher: name: George Mason University
Year: 2020

description: This study examined one possible barrier to the effectiveness of equitable team-based rewards (i.e. rewards allocated according to each team member’s individual contribution) that can especially occur with interdependent tasks, where it may be difficult to measure individual contributions: reward misallocation, such that higher performers are rewarded less than lower performers. The impact of three individual differences as moderators— equity sensitivity, exchange ideology, and agreeableness—was also examined. Results from a sample of 122 undergraduate students, each paired with a confederate teammate (who was always the lower performer), indicated that misallocation did not negatively impact individual performance or helping behaviors on a subsequent task. Misallocation, did however, reduce the intention to continue working with the teammate on future tasks. Lastly, moderating effects of the individual differences were not found.
Physical Activity Participation After a 16-Week Supervised Workplace Exercise RCT With a 15-Month Follow-Up
Creators: name: Hunter, Jayden
name: Gordon, Brett
name: Huynh, Minh
name: Benson, AC
Publisher: name: La Trobe
Year: 2022
Subjects: classificationCode: 3505
subject: Human resources and industrial relations
classificationCode: 42
subject: Health sciences
subject: FOS: Health sciences
classificationCode: 4202
subject: Epidemiology
classificationCode: 4206
subject: Public health

description: OBJECTIVE: To investigate the effects of exercise supervision on short- and longer-term moderate-vigorous physical activity (MVPA) participation. METHODS: Fifty-six Australian university employees completed a 16-week moderate-to-high intensity aerobic and resistance exercise intervention, and the international physical activity questionnaire (IPAQ) 15 months later. Participants received either personal (SUP; n = 21) or non-personal (NPS; n = 19) exercise supervision at an onsite facility or exercised offsite with no supervision (CON; n = 16). RESULTS: A linear mixed model identified a significant group × time interaction effect for MVPA, with increases at 15-month follow-up for CON only. Pooled data suggested more participants completed ≥500 MET-minutes of weekly MVPA at 16 weeks (66%) and 15-month follow-up (68%) compared to baseline (54%). CONCLUSIONS: A comprehensive health and fitness assessment and individually tailored exercise without personal supervision may promote ongoing MVPA.
title: Representing and Visualizing Articulated Movement
Creators: name: Revelle, Matthew
Publisher: name: George Mason University
Year: 2009
Subjects: subject: Movement
subject: Motion
subject: Animation
subject: Language
subject: Model
subject: Algebra

description: This thesis develops a representation and system of operations for articulated limb movement which can be used to describe both specific and general movements. A movement primitive in the representation can be described by the angle, axis, and accelerations of a joint rotation and may be combined with other movement primitives to create a new primitive or a sequence of primitives that simultaneously operate on the joints of a limb. The movement representation in this thesis provides a system for defining symbolic representations of movements. This system includes structured types for a single joint rotation, the sequencing of multiple joint rotations, and a limb model. To validate the system, an interactive environment with an integrated visualization of a body model has been developed. The environment is embedded in the Clojure programming language, a Lisp dialect that runs on the Java Virtual Machine. The jME scene graph based graphics engine is used to render the body model and visualize movement. The body model is constructed of mesh cylinders for body segments and graph nodes as joints. The environment allows a user to define movements, perform operations on movements, and apply a movement to the body model and view the effect in real time. Two complex movement examples are provided.
Development of a robust transient expression screening system in protoplasts of cannabis
Creators: name: Matchett-Oates, Lennon
name: Mohamaden, E
name: Spangenberg, German
name: Cogan, Noel
Publisher: name: La Trobe
Year: 2022
Subjects: classificationCode: 30
subject: Agricultural, veterinary and food sciences
classificationCode: 3001
subject: Agricultural biotechnology
subject: FOS: Agricultural biotechnology
classificationCode: 31
subject: Biological sciences
subject: FOS: Biological sciences
classificationCode: 3108
subject: Plant biology

description: Transient expression systems in mesophyll protoplasts have been utilised in many plant species as an indispensable tool for gene function analysis and efficacious genome editing constructs. However, such a system has not been developed in Cannabis due to the recalcitrant nature of the plant to tissue culture as well as its illegal status for many years. In this study, young expanding leaves from aseptic in vitro Cannabis explants were used for protoplast isolation. Factorial designs were used to optimise variables in viable protoplast isolation and transient expression of GFP, with a range analyses performed to determine, and quantify, significantly impacting variables. Viable protoplast yields as high as 5.7 × 106 were achieved with 2.5% (w/v) Cellulase R-10, 0.3% (w/v) Macerozyme R-10 and 0.7 M mannitol, incubated for 16 h. As indicated by the transient expression of GFP, efficiency reached 23.2% with 30 μg plasmid, 50% PEG, 1 × 106 protoplasts and a transfection duration of 20 min. Application of the optimised protocol for protoplast isolation was successfully evaluated on three subsequent unrelated genotypes to highlight the robustness and broad applicability of the developed technique.
Global Catalog of Extreme Convective Storms Derived from Cloud Water Data
Creators: name: Mielke, Christian A.
name: Springer, Anne
Publisher: name: Zenodo
Year: 2025
Subjects: Convective storms
Cloud water
TCCW
ERA5

description: Convective storms are associated with large variations in cloud water masses, which can be used to identify and classify such events. This catalog provides details on the location, timing, and size of the largest convective storms worldwide from 2002 to 2023. The ranking is based on total column cloud water (TCCW) events, which were derived by clustering global TCCW grid cells exceeding a surface mass density of 2 kg/m², utilizing a 3D-connected-component algorithm (Silversmith, 2021). The TCCW fields, with a grid resolution of 0.25° × 0.25° and one-hourly sampling, were derived from the ERA5 reanalysis data set (Hersbach et al., 2019) by subtracting total column water vapor (TCWV) from total column water (TCW). TCCW represents the sum of liquid water, cloud ice, rain and snow within a vertical column extending from the Earth’s surface to the top level of the atmosphere.

Additionally, files with polygons of the events displayed in the catalog are provided.
Development of a robust transient expression screening system in protoplasts of Cannabis
Creators: name: Matchett-Oates, Lennon
name: Mohamaden, E
name: Spangenberg, German
name: Cogan, Noel
Publisher: name: La Trobe
Year: 2022

description: Transient expression systems in mesophyll protoplasts have been utilised in many plant species as an indispensable tool for gene function analysis and efficacious genome editing constructs. However, such a system has not been developed in Cannabis due to the recalcitrant nature of the plant to tissue culture as well as its illegal status for many years. In this study, young expanding leaves from aseptic in vitro Cannabis explants were used for protoplast isolation. Factorial designs were used to optimise variables in viable protoplast isolation and transient expression of GFP, with a range analyses performed to determine, and quantify, significantly impacting variables. Viable protoplast yields as high as 5.7 × 106 were achieved with 2.5% (w/v) Cellulase R-10, 0.3% (w/v) Macerozyme R-10 and 0.7 M mannitol, incubated for 16 h. As indicated by the transient expression of GFP, efficiency reached 23.2% with 30 μg plasmid, 50% PEG, 1 × 106 protoplasts and a transfection duration of 20 min. Application of the optimised protocol for protoplast isolation was successfully evaluated on three subsequent unrelated genotypes to highlight the robustness and broad applicability of the developed technique.
Design of a clinical database to support research purposes: Challenges and solutions
Creators: name: Samra, Halima Edris
name: Li, Alice
name: Soh, Boon
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: The aim of this paper is to propose solutions to challenges faced by database systems for clinical research purposes. Current clinical databases are primarily based on data acquisition for healthcare intentions. However, these healthcare databases lack the data analysis capability for clinical researchers. In order for clinical researchers to use the healthcare databases in an effective manner, such as in their clinical trial studies, challenges of data integration, data storage, and data retrieval in the current healthcare database settings need to be overcome. Our proposed solutions include using: 1) NoSQL to efficiently integrate clinical databases with legacy healthcare databases, (2) entity attribute value model for data retrieval, and (3) warehouse for big data storage.
Detection of Harassment type of Cyberbullying: A Dictionary of Approach Words and Its Impact
Creators: name: Mahbub, Syed
name: Pardede, Eric
name: Kayes, A S M
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: The purpose of this paper is to analyse the effects of predatory approach words in the detection of cyberbullying and to propose a mechanism of generating a dictionary of such approach words. The research incorporates analysis of chat logs from convicted felons, to generate a dictionary of sexual approach words. By analysing data across multiple social networks, the study demonstrates the usefulness of such a dictionary of approach words in detection of online predatory behaviour through machine learning algorithms. It also shows the difference between the nature of contents across specific social network platforms. The proposed solution to detect cyberbullying and the domain of approach words are scalable to fit real-life social media, which can have a positive impact on the overall health of online social networks. Different types of cyberbullying have different characteristics. However, existing cyberbullying detection works are not targeted towards any of these specific types. This research is tailored to focus on sexual harassment type of cyberbullying and proposes a novel dictionary of approach words. Since cyberbullying is a growing threat to the mental health and intellectual development of adolescents in the society, models targeted towards the detection of specific type of online bullying or predation should be encouraged among social network researchers.
General Considerations for Conducting Online Qualitative Research and Practice Implications for Interviewing People with Acquired Brain Injury
Creators: name: Topping, Megan Ciara
name: Douglas, Jacinta
name: Winkler, Dianne
Publisher: name: La Trobe
Year: 2022
Subjects: classificationCode: 42
subject: Health sciences
subject: FOS: Health sciences
classificationCode: 4203
subject: Health services and systems

description: Qualitative methodology is key to understanding the lived experience of people with acquired brain injury (ABI). However, as demonstrated during the global pandemic (COVID-19), face-to-face interviewing is not always viable. This lack of availability has been particularly relevant for people with disability who are at increased risk of contracting the virus and experiencing poorer outcomes. Fortunately, advancing technologies provide increasing opportunities for communicating online, thus it is plausible for qualitative disability researchers to adapt to remote interviewing. People with ABI often experience varying degrees of cognitive and communication impairments and therefore require specific considerations in the planning of research projects. In this paper, we examine learnings from existing literature around online qualitative research, specifically for videoconference, focus groups and email-interviewing methods. The key aim is to map out the practical, ethical and methodological considerations when adapting research to an online environment. As interviewing people with ABI online has received little attention in the literature, learnings from broader disability populations and the general population inform much of the considerations. Thus, the suggestions for practice are likely to be relevant to a broader population, but specific implications for people with ABI are discussed. Overall, we propose that it is viable, and sometimes preferable, to utilize online interview techniques but researchers must take care to consider the practical, ethical and methodological implications of doing so.
General Considerations for Conducting Online Qualitative Research and Practice Implications for Interviewing People with Acquired Brain Injury
Creators: name: Topping, Megan Ciara
name: Douglas, Jacinta
name: Winkler, Dianne
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Qualitative methodology is key to understanding the lived experience of people with acquired brain injury (ABI). However, as demonstrated during the global pandemic (COVID-19), face-to-face interviewing is not always viable. This lack of availability has been particularly relevant for people with disability who are at increased risk of contracting the virus and experiencing poorer outcomes. Fortunately, advancing technologies provide increasing opportunities for communicating online, thus it is plausible for qualitative disability researchers to adapt to remote interviewing. People with ABI often experience varying degrees of cognitive and communication impairments and therefore require specific considerations in the planning of research projects. In this paper, we examine learnings from existing literature around online qualitative research, specifically for videoconference, focus groups and email-interviewing methods. The key aim is to map out the practical, ethical and methodological considerations when adapting research to an online environment. As interviewing people with ABI online has received little attention in the literature, learnings from broader disability populations and the general population inform much of the considerations. Thus, the suggestions for practice are likely to be relevant to a broader population, but specific implications for people with ABI are discussed. Overall, we propose that it is viable, and sometimes preferable, to utilize online interview techniques but researchers must take care to consider the practical, ethical and methodological implications of doing so.
Electroretinography and gene expression measures implicate phototransduction and metabolic shifts in chick myopia and hyperopia models
Creators: name: Riddell, Nina
name: Murphy, Melanie
name: Crewther, Sheila
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: The Retinal Ion-Driven Fluid Efflux (RIDE) model theorizes that phototransduction-driven changes in trans-retinal ion and fluid transport underlie the development of myopia (short-sightedness). In support of this model, previous functional studies have identified the attenuation of outer retinal contributions to the global flash electroretinogram (gfERG) following weeks of myopia induction in chicks, while discovery-driven transcriptome studies have identified changes to the expression of ATP-driven ion transport and mitochondrial metabolism genes in the retina/RPE/choroid at the mid-to late-induction time-points. Less is known about the early time-points despite biometric analyses demonstrating changes in eye growth by 3 h in the chick lens defocus model. Thus, the present study compared gfERG and transcriptome profiles between 3 h and 3 days of negative lens-induced myopia and positive lens-induced hyperopia in chicks. Photoreceptor (a-wave and d-wave) and bipolar (b-wave and late-stage d-wave) cell responses were suppressed following negative lens-wear, particularly at the 3–4 h and 3-day time-points when active shifts in the rate of ocular growth were expected. Transcriptome measures revealed the up-regulation of oxidative phosphorylation genes following 6 h of negative lens-wear, concordant with previous reports at 2 days in this model. Signal transduction pathways, with core genes involved in glutamate and G-protein coupled receptor signalling, were down-regulated at 6 h. These findings contribute to a growing body of evidence for the dysregulation of phototransduction and mitochondrial metabolism in animal models of myopia.
Unternehmensverantwortung von Facebook für die Verbreitung von Falschinformation
Creators: name: Ehrbar Christian
name: Niedermann Florian
name: Spasic Biljana
name: Samuel, Jost
Publisher: name: Zenodo
Year: 2022
Subjects: Economics
Sustainable Development Goals
Facebook
Social Media
Fake News

description: A paper discussing the responsibilty of the social media Plattform Facebook in halting the spread of Fake News.
Continuity of care experiences: A national cross-sectional survey exploring the views and experiences of Australian students and academics
Creators: name: Newton, Michelle
name: Faulks, Fiona
name: Bailey, Carolyn
name: Davis, Jennifer
name: Vermeulen, Monique
name: Tremayne, Anne
name: Kruger, Gina
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: No description supplied
Structure and dynamics of the PAC1R in different conformational states
Creators: name: Nettleton, Theodore Jenkins
Publisher: name: Monash University
Year: 2024
Subjects: classificationCode: 310112
subject: Structural biology (incl. macromolecular modelling)
classificationCode: 321499
subject: Pharmacology and pharmaceutical sciences not elsewhere classified

description: The pituitary adenylate cyclase-activating polypeptide 1 receptor (PAC1R) is a G protein-coupled receptor that is found in the outer membrane of cells, in particular in the central nervous system. From there, it can recognise and communicate signals from the outside to the inside of a cell, in order to regulate physiological processes including pain, stress, and learning. However, the specific molecular mechanisms that allow PAC1R to do this are not fully understood. This PhD seeks to remedy this by investigating the shape, structure, and movements of PAC1R, to better understand how this receptor functions.
Podiatrist-delivered health coaching to facilitate the use of a smart insole to support foot health monitoring in people with diabetes-related peripheral neuropathy
Creators: name: Macdonald, Emma
name: Perrin, Byron
name: Cleeland, L
name: Kingsley, Michael
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: This trial evaluated the feasibility of podiatrist-led health coaching (HC) to facilitate smart-insole adoption and foot monitoring in adults with diabetes-related neuropathy. Adults aged 69.9 ± 5.6 years with diabetes for 13.7 ± 10.3 years participated in this 4-week explanatory sequential mixed-methods intervention. An HC training package was delivered to podiatrists, who used HC to issue a smart insole to support foot monitoring. Insole usage data monitored adoption. Changes in participant understanding of neuropathy, foot care behaviours, and intention to adopt the smart insole were measured. Focus group and in-depth interviews explored quantitative data. Initial HC appointments took a mean of 43.8 ± 8.8 min. HC fidelity was strong for empathy/rapport and knowledge provision but weak for assessing motivational elements. Mean smart-insole wear was 12.53 ± 3.46 h/day with 71.2 ± 13.9% alerts not effectively off-loaded, with no significant effect for time on usage F(3,6) = 1.194 (p = 0.389) or alert responses F(3,6) = 0.272 (p = 0.843). Improvements in post-trial questionnaire mean scores and focus group responses indicate podiatrist-led HC improved participants’ understanding of neuropathy and implementation of footcare practices. Podiatrist-led HC is feasible, supporting smart-insole adoption and foot monitoring as evidenced by wear time, and improvements in self-reported footcare practices. However, podiatrists require additional feedback to better consolidate some unfamiliar health coaching skills. ACTRN12618002053202.
Broad-based influenza-specific CD8+ T cell response without the typical immunodominance hierarchy and its potential implication
Creators: name: Huang, Miaojuan
name: Xu, Rong
name: Triffon, Cristina
name: Mifsud, N
name: Chen, Weisan
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Syngeneic murine systems have pre-fixed MHC, making them an imperfect model for investigating the impact of MHC polymorphism on immunodominance in influenza A virus (IAV) infections. To date, there are few studies focusing on MHC allelic differences and its impact on immunodominance even though it is well documented that an individual’s HLA plays a significant role in determining immunodominance hierarchy. Here, we describe a broad-based CD8+ T cell response in a healthy individual to IAV infection rather than a typical immunodominance hierarchy. We used a systematic antigen screen approach combined with epitope prediction to study such a broad CD8+ T cell response to IAV infection. We show CD8+ T cell responses to nine IAV proteins and identify their minimal epitope sequences. These epitopes are restricted to HLA-B*44:03, HLA-A*24:02 and HLA-A*33:03 and seven out of the nine epitopes are novel (NP319–330# (known and demonstrated minimal epitope positions are subscripted; otherwise, amino acid positions are shown as normal text (for example NP 319–330 or NP 313–330)), M1124–134, M27–15, NA337–346, PB239–49, HA445–453 and NS1195–203 ). Additionally, most of these novel epitopes are highly conserved among H1N1 and H3N2 strains that circulated in Australia and other parts of the world.
Protein purification and crystallization of HLA-A∗02:01 in complex with SARS-CoV-2 peptides
Creators: name: Chatzileontiadou, Dimitra
name: Szeto, Christopher
name: Jayasinghe, D
name: Gras, Stephanie
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Understanding T-cell responses requires identifying viral peptides presented by human leukocyte antigens (HLAs). X-ray crystallography can be used to visualize their presentation. This protocol describes the expression, purification, and crystallization of HLA-A∗02:01, one of the most frequent HLA in the global population in complex with peptides derived from the SARS-CoV-2 nucleocapsid protein. This protocol can be applied to different HLA class I molecules bound to other peptides. For complete details on the use and execution of this protocol, please refer to Szeto et al. (2021).
title: The Blazing Field
Creators: name: Wattenberg, Madeleine
Publisher: name: George Mason University
Year: 2022
Subjects: subject: Poetry

description: This thesis has been embargoed for 5 years and will not be available until April 2022 at the earliest.
description: Drawing from the perhaps antithetical languages provided by myth and science, the poems in this collection examine how women become socially constructed through cultural narratives. Toward this end, the poems questions women’s bodies as culturally scripted boundaries by reimagining Greco-Roman myths of bodily transformation. At times, this questioning occurs through a contemporary speaker’s relationship with her scientist father as she seeks to understand different modes of knowledge and the consequences of these modes on how we establish connection to others, the world, and ourselves. Underlying these themes rests the question of how language and utterance form the boundary of a gendered body.
Supplementary document for Numerical investigation of gas-filled multipass cells in the enhanced dispersion regime for clean spectral broadening and pulse compression - 6426052.pdf
Creators: name: Staels, Victor Segundo
name: Jarque, Enrique Conejero
name: Carlson, Daniel
name: Hemmer, Michaël
name: Kapteyn, Henry
name: Murnane, Margaret
name: San Roman, Julio
Publisher: name: Optica Publishing Group
Year: 2025
Subjects: Uncategorized

description: Supplement 1
Structural basis of coronavirus E protein interactions with human PALS1 PDZ domain
Creators: name: Javorsky, Airah Joyce
name: Humbert, Patrick
name: Kvansakul, Marc
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: SARS-CoV-2 infection leads to coronavirus disease 2019 (COVID-19), which is associated with severe and life-threatening pneumonia and respiratory failure. However, the molecular basis of these symptoms remains unclear. SARS-CoV-1 E protein interferes with control of cell polarity and cell-cell junction integrity in human epithelial cells by binding to the PALS1 PDZ domain, a key component of the Crumbs polarity complex. We show that C-terminal PDZ binding motifs of SARS-CoV-1 and SARS-CoV-2 E proteins bind the PALS1 PDZ domain with 29.6 and 22.8 μM affinity, whereas the related sequence from MERS-CoV did not bind. We then determined crystal structures of PALS1 PDZ domain bound to both SARS-CoV-1 and SARS-CoV-2 E protein PDZ binding motifs. Our findings establish the structural basis for SARS-CoV-1/2 mediated subversion of Crumbs polarity signalling and serve as a platform for the development of small molecule inhibitors to suppress SARS-CoV-1/2 mediated disruption of polarity signalling in epithelial cells.
DEVELOPMENT OF AN OFF-LINE RAINFLOW COUNTING ALGORITHM WITH TEMPORAL PARAMETERS AND DATA REDUCTION
Creators: name: Twomey, James Matthew
Publisher: name: Digital Repository at the University of Maryland
Year: 2016
Subjects: subject: Mechanical engineering
subject: FOS: Mechanical engineering
subject: dwell time
subject: fatigue
subject: Rainflow counting

description: Rainflow counting methods convert a complex load time history into a set of load reversals for use in fatigue damage modeling. Rainflow counting methods were originally developed to assess fatigue damage associated with mechanical cycling where creep of the material under load was not considered to be a significant contributor to failure. However, creep is a significant factor in some cyclic loading cases such as solder interconnects under temperature cycling.  In this case, fatigue life models require the dwell time to account for stress relaxation and creep.

This study develops a new version of the multi-parameter rainflow counting algorithm that provides a range-based dwell time estimation for use with time-dependent fatigue damage models.  To show the applicability, the method is used to calculate the life of solder joints under a complex thermal cycling regime and is verified by experimental testing.

An additional algorithm is developed in this study to provide data reduction in the results of the rainflow counting. This algorithm uses a damage model and a statistical test to determine which of the resultant cycles are statistically insignificant to a given confidence level. This makes the resulting data file to be smaller, and for a simplified load history to be reconstructed.