Additional file 1 of Screening for efficient nitrogen sources for overproduction of the biomass of the functionally probiotic L. plantarum strain RPR42 in a cane molasses-based medium
Creators: name: Moslem Papizadeh
name: Rohani, Mahdi
name: Hosseini, Seyed Nezamedin
name: Shojaosadati, Seyed Abbas
name: Nahrevanian, Hossein
name: Malihe Talebi
name: Pourshafie, Mohammad Reza
Publisher: name: figshare
Year: 2020
Subjects: subject: Space Science
subject: Microbiology
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Biotechnology
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: Plant Biology

description: Additional file 1: Figure S1. The biomass production of strain RPR42 in a gradient of CSL concentrations. The averages for the triplicate dry biomass weights are presented and finally values rounded to the nearest 0.00-0.09. Error bars represent the standard error. Figure S2. The biomass production of strain RPR42 in a gradient of WGE concentrations. The averages for the triplicate dry biomass weights are presented and finally values rounded to the nearest 0.00-0.09. Error bars represent the standard error. Figure S3. The biomass production of strain RPR42 in a gradient of Cheese whey concentrations. The averages for the triplicate dry biomass weights are presented and finally values rounded to the nearest 0.00-0.09. Error bars represent the standard error. Figure S4. Optimal concentration of polysorbates, Tween 20, 40, 60, and 80, in the presence of 10% and 7.5% (v/v) of cane molasses and CSL, respectively. The averages for the triplicate dry biomass weights are presented and finally values rounded to the nearest 0.00-0.09. Error bars represent the standard error. Figure S5. A factorial design for studying the influence of Tween 20, 40, 60, and 80 (each in a single level) on the biomass production of strain RPR42 in a medium in which the concentration of cane molasses and CSL were 10% and 7.5% (v/v), respectively. The averages for the triplicate dry biomass weights are presented and finally values rounded to the nearest 0.00-0.09. Figure S6. The influence of polysorbates (Tween 20, 40, 60, and 80) on the biomass production of strain RPR42 in the presence of cane molasses (15%) and WGE (70%). Error bars represent the standard error. Figure S7. The effects of the polysorbates, Tween 20, 40, 60, and 80 (each in a single level), on the biomass production of strain RPR42 in a factorial experiment. The concentration of cane molasses and WGE were 15% (v/v) and 70% (v/v), respectively. The averages for the triplicate dry biomass weights are presented and finally values rounded to the nearest 0.00-0.09. Figure S8. Optimal concentration of polysorbates; Tween 20, 40, 60, and 80 for the growth of strain RPR42 in the presence of cane molasses (10% (v/v)) and cheese whey (5% (w/v)). Error bars represent the standard error. Figure S9. The influence of the polysorbates; Tween 20, 40, 60, and 80 (each in a single level), on the biomass production of strain RPR42 in a factorial design. The concentration of cane molasses and cheese whey were 10% (v/v) and 5% (w/v), respectively. The averages for the triplicate dry biomass weights are presented and finally values rounded to the nearest 0.00-0.09. Figure S10. The influence of casein hydrolyzate and glucose on the growth of strain RPR42. The concentration of cane molasses was: 10% (v/v) in CSL-C, CSL-G, Whey-C and Whey-G; and it was 15% in WGE-C and WGE-G. CSL concentration was 7.5% (v/v) in CSL-C and CSL-G media. WGE concentration was 70% (v/v) in WGE-C and WGE-G media. Cheese whey concentration in Whey-C and Whey-G media was 5% (w/v). CSL-C, WGE-C, Whey-C contained casein hydrolysate and CSL-G, WGE-G and Whey-G contained glucose. Error bars represent the standard error. Table S1. The full factorial designs (42) used for optimization of the nitrogenous sources and cane molasses solutions for biomass production of strain RPR42. The concentrations of all ingredients were in percent (cane molasses and CSL; (v/v), Cheese whey; (w/v)). Table S2. Partial factorial design for screening the effects of glucose, casein hydrolysate, cane molasses, CSL, WGE, cheese whey, and polysorbates (A; Casein hydrolysate, B; Cane molasses; C; CSL, D; WGE, E; Cheese whey, F; Glucose, G; 1:1:1 mixture of Tween 20, 60, and 80 polysorbates). Table S3. The influence of the five mineral components (each in a single level) on the biomass production of strain RPR42 in a factorial experiment design. The cane molasses and CSL concentrations were 15% and 7.5% (v/v), respectively. Concentration (g/L) of the minerals were: 4; di-ammonium citrate, 7; sodium acetate, 5; K2HPO4, 0.3; MgSO4, and 0.05; MnSO4. The averages for the triplicate dry biomass weights are presented and finally values rounded to the nearest 0.00-0.09. Table S4. Factorial design experiment screening the influence of the five mineral components (each in a single level) on the biomass production of strain RPR42. The concentration of cane molasses and WGE were 15% and 70% (v/v). The concentration of cane molasses and cheese whey were 10% and 5% (v/v), respectively. Concentration (g/L) of the minerals were: 4; di-ammonium citrate, 7; sodium acetate, 5; K2HPO4, 0.3; MgSO4, and 0.05; MnSO4. The averages for the triplicate dry biomass weights are presented and finally values rounded to the nearest 0.00-0.09. Table S5. Factorial design for assessment of the effects of mineral components (each in a single level) on the biomass production of strain RPR42 in a medium solution in which the concentration of cane molasses and cheese whey were 10% and 5% (w/v), respectively. Concentration (g/L) of the minerals were: 4; di-ammonium citrate, 7; sodium acetate, 5; K2HPO4, 0.3; MgSO4, and 0.05; MnSO4. The averages for the triplicate dry biomass weights are presented and finally values rounded to the nearest 0.00-0.09.