Limosilactobacillus reuteri SUBSP. PORCINUS 2021, SUBSP. NOV.
Creators: name: Li, Fuyong
name: Cheng, Christopher C.
name: Zheng, Jinshui
name: Liu, Junhong
name: Quevedo, Rodrigo Margain
name: Li, Junjie
name: Roos, Stefan
name: Gänzle, Michael G.
name: Walter, Jens
Publisher: name: Zenodo
Year: 2021
Subjects: Biodiversity
Taxonomy
Bacteria
Firmicutes
Bacilli
Lactobacillales
Lactobacillaceae
Limosilactobacillus
Limosilactobacillus reuteri

description: DESCRIPTION OF LIMOSILACTOBACILLUS REUTERI SUBSP. PORCINUS SUBSP. NOV.   
 Limosilactobacillus reuteri subsp. porcinus (por.ci′ nus. L. masc. adj. porcinus of swine, referring to the host origin of most strains of this subspecies being swine).   
 L. reuteri strains clustered in lineage V (Fig. 3) belong to L. reuteri subsp. porcinus and they were isolated from pigs [5, 7]. Strains (3c6 T and 20-2) of this subspecies have an ANI value of 99.1% with each other and ANI values of 93.8–96.6% with other L. reuteri strains belonging to different subspecies (Fig. 4). Acid is produced from D-ribose, D-galactose, D-glucose, maltose, lactose, melibiose, sucrose, raffinose and potassium gluconate; acid production from methyl-α- D-glucopyranoside is strain-specific; acid is not produced from L-arabinose, D-xylose, D-fructose, D-mannose, aesculin, glycerol, erythritol, D-arabinose,L-xylose,D-adonitol, methyl β -Dxylopyranoside, L-sorbose,L-rhamnose, dulcitol, inositol, D-mannitol, D-sorbitol, methyl α- D-mannopyranoside, N -acetylglucosamine, amygdalin, arbutin, salicin, cellobiose, trehalose, inulin, melezitose, starch, glycogen, xylitol, gentiobiose, turanose, D-lyxose, D-tagatose, D-fucose, L-fucose,D-arabitol,L-arabitol, potassium 2-ketogluconate or potassium 5-ketogluconate. Phylogenetic analyses based on the core genes identified in this study (Fig. 3) and previous studies [5, 43, 46], AFLP and MLSA (using concatenated sequences of ddl, pkt, leuS, gyrB, dltA, rpoA and recA genes) [7] indicate that strains clustered in this lineage are pig-specific. Both 3c6 T and 20-2 possess the pdu-cbi-cob-hem cluster (pdu cluster) [5, 46], which equips them with the ability to utilize 1,2-propanediol and glycerol as electron acceptors [16, 39, 40] and to produce the antimicrobial compound reuterin [8].   
 The type strain, 3c6 T (=DSM 110571 T =LMG 31635 T), was isolated from porcine gastrointestinal tract [7, 46], with a DNA G+C content of 38.6mol%.
description: Published as part of Li, Fuyong, Cheng, Christopher C., Zheng, Jinshui, Liu, Junhong, Quevedo, Rodrigo Margain, Li, Junjie, Roos, Stefan, Gänzle, Michael G. & Walter, Jens, 2021, Limosilactobacillus balticus sp. nov., Limosilactobacillus agrestis sp. nov., Limosilactobacillus albertensis sp. nov., Limosilactobacillus rudii sp. nov. and Limosilactobacillus fastidiosus sp. nov., five novel Limosilactobacillus species isolated from the vertebrate gastrointestinal tract, and proposal of six subspecies of Limosilactobacillus reuteri adapted to the gastrointestinal tract of specific vertebrate hosts, pp. 1-21 in International Journal of Systematic and Evolutionary Microbiology (004644) (004644) 71 (2) on page 18, DOI: 10.1099/ijsem.0.004644, http://zenodo.org/record/6048739