Additional file 1 of Integration of metabolites from meta-analysis with transcriptome reveals enhanced SPHK1 in PDAC with a background of pancreatitis
Creators: name: Ketavarapu, Vijayasarathy
name: Ravikanth, Vishnubhotla
name: Sasikala, Mitnala
name: Rao, G. V.
name: Devi, Ch. Venkataramana
name: Sripadi, Prabhakar
name: Bethu, Murali Satyanarayana
name: Amanchy, Ramars
name: Murthy, H. V. V.
name: Pandol, Stephen J.
name: Reddy, D. Nageshwar
Publisher: name: figshare
Year: 2022
Subjects: subject: Biochemistry
subject: Space Science
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Cancer
subject: Plant Biology

description: Additional file 1: Fig. S1. Publication bias and Sensitivity plots (A) Funnel plot of metabolomic studies (B) Meta-analysis based on reported sensitivity among 10 studies. Heterogenity (I2) was assessed for fixed and random effects (C) Publication bias computed from AUC and calculated standard error among 19 studies. Fig. S2. Representative heat map of transcriptome datasets (A) Significant (P-value ≤ 0.05) dysregulated genes in tissues with Chronic pancreatitis compared to Controls sorted B) Significant (P-value ≤ 0.05) dysregulated genes in tissues with PDAC with a background of chronic pancreatitis compared to Controls, (C) Significant (P-value ≤ 0.05) dysregulated genes in tissues with Pancreatic ductal adenocarcinoma compared to Controls. Red and green colour depict up and downregulated genes. Fig. S3. Transcriptomics (A) Splicing index of 3 upregulated genes PIGC(21.63), PKM(-4.92), PPIB(3.51) in CP and PDAC-CP tissues. Red and green lines represent splicing index between CP and PDAC-CP for each gene respectively (B) Heatmap of 3 tumor suppressor genes AZGP1, EGLN1 and GNMT in CP and PDAC-CP tissues. Fig. S4. Gene ontology of 102 genes that were common between CP and PDAC-CP (A) Molecular function of corresponding genes of altered metabolites identified in CP and PDAC and transcriptomes of CP, PDAC-CP (B) Biological processes associated with genes identified in CP, PDAC metabolome and CP, PDAC-CP transcriptomes (C) Cellular component distribution for genes identified in CP, PC metabolome and CP, PDAC-CP transcriptomes (D) Protein class distribution for genes identified in CP, PC metabolomics and CP, PDAC-CP transcriptomes (E) Pathway analysis for genes identified in CP, PC metabolomes and CP, PDAC-CP transcriptomes. Table S1. MOOSE guidelines. Table S2. Characteristics of patients for pancreatic tissue transcriptomes. Table S3. Dysregulated genes in Chronic pancreatitis(CP) and Pancreatic ductal adenocarcinoma with a background of chronic pancreatitis(PDAC-CP), tumor suppressor genes are highlighted in yellow color. Table S4. Dysregulated genes in Pancreatic ductal adenocarcinoma with a background of chronic pancreatitis(PDAC-CP) and PDAC, tumor suppressor genes are highlighted in yellow color. Table S5. Fisher’s exact test between CP, PDAC-CP and PC groups.