Additional file 1 of Effect of testosterone therapy on breast tissue composition and mammographic breast density in trans masculine individuals
Creators: name: Heng, Yujing J.
name: Baker, Gabrielle M.
name: Fein-Zachary, Valerie J.
name: Guzman-Arocho, Yaileen D.
name: Bret-Mounet, Vanessa C.
name: Massicott, Erica S.
name: Torous, Vanda F.
name: Schnitt, Stuart J.
name: Gitin, Sy
name: Russo, Paul
name: Tobias, Adam M.
name: Bartlett, Richard A.
name: Varma, Gopal
name: Kontos, Despina
name: Yaghjyan, Lusine
name: Irwig, Michael S.
name: Potter, Jennifer E.
name: Wulf, Gerburg M.
Publisher: name: figshare
Year: 2024
Subjects: subject: Biophysics
subject: Biochemistry
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Science Policy
subject: Plant Biology
subject: FOS: Biological sciences

description: Additional file 1. Supplementary 1 A. Automated breast tissue composition significantly correlated with pathologists’ assessments. The percentage (%) of epithelium (A) significantly inversely correlated with increasing degrees of lobular atrophy (p &lt; 0.001; Spearman’s rho). Cases where the pathologists classified the stroma as predominantly fibrous or fatty were significantly correlated with higher % of fibrous stroma (B; p &lt; 0.001) or fat (C ; p &lt; 0.001), respectively. Each box displays the median, and 25 th and 75 th percentiles (upper and lower hinges). The lower whisker represents the smallest observation greater than or equal to the lower hinge - 1.5 * inter quartile range (IQR); the upper whisker represents the largest observation less than or equal to upper hinge + 1.5 * IQR. B. Automated breast tissue composition remained significantly correlated with pathologists’ assessments even when stratified by testosterone therapy (TT) (all p &lt; 0.001). Spearman’s rho, 95% confidence interval (CI), and p -values comparing the percentage (%) of each tissue region and pathologists’ assessments in A , B , and C , are displayed in D . Each box displays the median, and 25 th and 75 th percentiles (upper and lower hinges). The lower whisker represents the smallest observation greater than or equal to the lower hinge − 1.5 * inter quartile range (IQR); the upper whisker represents the largest observation less than or equal to upper hinge + 1.5 * IQR. Supplementary 2. The association of testosterone therapy (per six months duration) and the percentages (%) of each breast tissue region, additionally adjusting for alcohol consumption in the fully adjusted model 3. Supplementary 3. The association of testosterone therapy (per six months duration) and the percentages (%) of each breast tissue region after excluding the nine subjects with atypical lesions. Supplementary 4. The association of testosterone therapy (per six months duration) and the percentages (%) of each breast tissue region among users who administered testosterone via intra-muscular injection and non-testosterone users. Supplementary 5. The association of testosterone therapy (per six months duration) and the percentages (%) of each breast tissue region and among nulliparous subjects. Supplementary 6. The association of testosterone therapy (per six months duration) and the percentages (%) of each breast tissue region, stratified by body mass index (BMI). Supplementary 7. The association of testosterone therapy use (users/non-users) and the percentages (%) of each breast tissue region. Supplementary 8. Characteristics of 42 transmasculine individuals who had mammography prior to chest contouring surgery. Supplementary 9. Laboratory for Individualized Breast Radiodensity Assessment (LIBRA) breast percent density (PD; B ), absolute dense area (C), and absolute non-dense area (D) significantly correlated with the radiologist’s breast tissue density assessment. Each box displays the median, and 25 th and 75 th percentiles (upper and lower hinges). The lower whisker represents the smallest observation greater than or equal to the lower hinge − 1.5 * inter quartile range (IQR); the upper whisker represents the largest observation less than or equal to upper hinge + 1.5 * IQR. Confidence interval; CI. Supplementary 10. The association between testosterone therapy (per six months duration) and Laboratory for Individualized Breast Radiodensity Assessment (LIBRA) measures. Supplementary 11. The association between testosterone therapy (per six months duration) and Laboratory for Individualized Breast Radiodensity Assessment (LIBRA) measures, stratified by body mass index (BMI). Supplementary 12. The association between testosterone therapy (per six months duration) and Laboratory for Individualized Breast Radiodensity Assessment (LIBRA) measures among nulliparous subjects. Supplementary 13. Scatterplot matrix correlating automated breast tissue composition (pink boxes) with Laboratory for Individualized Breast Radiodensity Assessment (LIBRA) measures (grey boxes) among 23 subjects. Supplementary 14. Scatterplot matrix correlating automated breast tissue composition data (pink boxes) with Laboratory for Individualized Breast Radiodensity Assessment (LIBRA) measures (grey boxes) among 17 subjects who had their chest-contouring surgeries within six months of their mammography.