Additional file 1 of Group 2 innate lymphoid cells are numerically and functionally deficient in the triple transgenic mouse model of Alzheimer’s disease
Creators: name: Fung, Ivan Ting Hin
name: Zhang, Yuanyue
name: Shin, Damian S.
name: Sankar, Poornima
name: Sun, Xiangwan
name: D’Souza, Shanti S.
name: Song, Renjie
name: Kuentzel, Marcy L.
name: Chittur, Sridar V.
name: Zuloaga, Kristen L.
name: Yang, Qi
Publisher: name: figshare
Year: 2021
Subjects: subject: Medicine
subject: Cell Biology
subject: Neuroscience
subject: Physiology
subject: FOS: Biological sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: Cancer
subject: 111714 Mental Health
subject: FOS: Health sciences
subject: 110309 Infectious Diseases

description: Additional file 1: Fig. S1. Characterization of ILC2 in 7-month old 3xTg-AD and control wild-type mice. (A) Representative flow cytometric profiles depicting GATA3 and T1/ST2 expression in ILC2 from the whole brain tissue of 7-month old female and male 3xTg-AD mice and age and sex-matched control wildtype mice. Plots were pre-gated on ILC2 (Lin-CD45+Thy1+T1/ST2 (B) Mean fluorescence intensity (MFI) of GATA3 and T1/ST2 expression in ILC2 from the whole brain tissue of 7-month old female and male 3xTg-AD mice and sex and age-matched control wildtype mice. Data are from 6 mice per group, two independent experiments. (C) Numbers of ILC2 in the lungs of 7-month old female and male 3xTg-AD mice and control wildtype mice. Data are from 4 mice per group, 2 independent experiments. Sex effect: F [1, 12] = 42.49, p&lt;0.01; Strain effect: F [1, 12] = 0.23, p = 0.64; Interaction: F [1, 12] = 0.23, p = 0.64. (D) scRNA-seq was performed with cultured ILC2 from 7-month old female and male 3xTg-AD mice and age and sex-matched wildtype control mice, after 7 days of culture. Violin plots depict the expression of the indicated genes. Error bars = mean ± SEM. **p&lt;0.01; n.s., not significant. Fig. S2. Immunofluorescence staining of 7-month old 3xTg-AD mice treated with PBS or IL-5. 7-month old female and male 3xTg-AD mice were treated with IL-5 or PBS daily for 2 days. Brain sections were obtained at 24 hours after treatment and stained with 6E10 antibodies. Representative immunofluorescence images were shown. Brain sections of 12-month old female 3xTg-AD mice were used as positive controls. Data represent 4 female and male mice per group, two independent experiments. Fig. S3. Behavior test results of 7-month old female wildtype control mice that were treated with IL-5 or PBS control. 7-month old female wildtype control mice were treated with IL-5 and PBS, and underwent behavior tests as described in Fig. 5A. (A) The Open Field arena was virtually divided into “Center”, “Periphery”, and “Corner” zones in Open Field test. (B) Concentrations of IL-5 in the cerebrospinal fluid (CSF) were examined in 7-month old female wildtype mice, at 24 hours after treatment with IL-5 or PBS daily for 2 days. Data are from 6 mice per group, two independent experiments. (C) Total distance traveled, and percentages of time spent in the indicated zones in the Open Field test. (D) Percentages of time spent in the closed arms in the Elevated Plus Maze test. (E) Percentages of time spent in the novel (N) and familiar (F) arms, and the percentages of entries into the novel and familiar arms, in the Y-maze forced alternation test. Percentage of time: PBS-treated t [18] = 5.47, p&lt;0.01, IL-5-treated t [18] = 5.86, p&lt;0.01. Percentage of entries: PBS-treated t [18] = 7.1, p&lt;0.01, IL-5-treated t [18] = 3.66, p&lt;0.01. (F) Escape latency and escape distance to the hidden platform, and the thigmotaxis rate during the 4 days of trials in the Water Maze test. Data are from 10 mice per group (C-F), 2 independent experiments. Error bars = mean ± SEM. **p&lt;0.01; n.s., not significant. Fig. S4. Behavior test results of 7-month old 3xTG-AD mice 4 weeks after treatment with IL-5 or PBS control. 7-month old female 3xTG-AD mice were treated with IL-5 and PBS and were rested for 5 weeks, then underwent behavior tests. (A) Experimental scheme for cytokine treatment and behavior tests. OF, Open Field; EPM, Elevated Plus Maze. (B) Total distance traveled, and the percentages of time spent in the indicated zones by Open Field test. (C) Percentages of time spent in the closed arms in the Elevated Plus Maze test. (D) Percentages of time spent in the novel (N) and familiar (F) arms, and the percentages of entries into the novel and familiar arms, in the Y-maze forced alternation test. (E) Escape latency and escape distance to the hidden platform, and the thigmotaxis rate during the 4 days of trials in the Water Maze test. Data are from 10 mice per group, 2 independent experiments. Error bars = mean ± SEM; n.s., not significant.