Supplementary materials
Creators: name: Fang, Chun
Publisher: name: figshare
Year: 2024
Subjects: classificationCode: 300903
subject: Veterinary bacteriology

description: <b>Fig 1. Growth curves of ScottA, Δ</b><b><i>galE</i></b><b> and CΔ</b><b><i>galE</i></b><b> in BHI medium.</b><b>Fig 2. Distribution of InlB after</b><b><i> galE</i></b><b> deletion.</b> (A) Chemiluminescent development image. (B) Grayscale value analysis result. Statistical analyses were carried out by t-test: *<i>P</i>≤0.05, **<i>P</i>≤0.01, ns means not significant.<b>Fig 3. Adhesion and invasion of ScottA, Δ</b><b><i>galE</i></b><b> and CΔ</b><b><i>galE</i></b><b> to Caco-2 and MGC803 cells. </b>(A) Adhesion to Caco-2 cells. (B) Invasion to Caco-2 cells. (C) Adhesion to MGC803 cells. (D) Invasion to MGC803 cells.<b> </b>The experiments were conducted in triplicate, and data were expressed as mean ± SD. Statistical analyses were carried out by t-test: *<i>P</i>≤0.05, **<i>P</i> ≤ 0.01.<b>Fig 4. </b><b>Phagocytosis</b><b> and proliferation of ScottA, Δ</b><b><i>galE</i></b><b> and CΔ</b><b><i>galE</i></b><b> in RAW264.7 cells. </b>(A) Phagocytosis in RAW264.7 cells. (B) Proliferation in RAW264.7 cells. The experiments were conducted in triplicate, and data were expressed as mean ± SD. Statistical analyses were carried out by t-test: *<i>P</i>≤0.05, **<i>P</i>≤ 0.01.<b>Fig 5. Bacterial loads of in the (A) liver, (B) spleen and (C) brain of mice infected with ScottA, Δ</b><b><i>galE</i></b><b> and CΔ</b><b><i>galE</i></b><b>. </b>Statistical analyses were carried out by Mann-Whitney test: **<i>P≤</i>0.01.<b>Fig 6. Survival curves of mice infected with ScottA, Δ</b><b><i>galE</i></b><b> and CΔ</b><b><i>galE</i></b><b>.</b><b>Fig 7. Histopathological changes in the liver, spleen and brain of mice after infection with ScottA, Δ</b><b><i>galE</i></b><b> or CΔ</b><b><i>galE</i></b><b>, or without infection.</b> The white arrows in the figure indicate the lesion area or cells.<b>Table S1. Primers for constructing galE gene deletion and complementation strains.</b><b>Table S2. Supplementary information for animal experiments (According to the ARRIVE guidelines for the reporting of animal).</b><b>Fig S1. Growth curves of </b><b><i>Listeria monocytogenes</i></b><b> (A) EGDe (Serotype 1/2a), (B) HumLm4 (Serotype 1/2c), (C)</b> <b>HumLm6 (Serotype 1/2b) and (D) 850658-prfA</b><sub><strong>M7</strong></sub><b> (Serotype 4a) in BHI medium.</b><b>Fig S2. The distribution of InlB in </b><b><i>Listeria monocytogenes</i></b><b> (A) EGDe (Serotype 1/2a), (B) HumLm6 (Serotype 1/2b), (C)</b> <b>HumLm4 (Serotype 1/2c) and (D) 850658-prfAM7 (Serotype 4a) after </b><b><i>galE</i></b><b> deletion.</b> Statistical analyses were carried out by the t-test: *<i>P</i>≤0.05, ns means not significant.<b>Fig S3. The overall profiles of both (A) cell wall proteins and (B) supernatant proteins.</b><b>Fig S4. The bacterial loads in the liver and spleen of mice infected with </b><b><i>Listeria monocytogenes</i></b><b> (A) EGDe (Serotype 1/2a), (B) HumLm6 (Serotype 1/2b) and (C) HumLm4 (Serotype 1/2c) after </b><b><i>galE</i></b><b> deletion.</b> Statistical analyses were carried out by Mann-Whitney test: *<i>P</i>≤0.05, **<i>P</i>≤ 0.01, ns means not significant.<br>