title: ÐÐ·ÑÑÐµÐ½Ð¸Ðµ ÐºÐ¾Ð»Ð»ÐµÐºÑÐ¸Ð¾Ð½Ð½ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð² Ð¼Ð¸ÐºÑÐ¾Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð¾Ð² Ð´Ð»Ñ ÑÐ¾Ð·Ð´Ð°Ð½Ð¸Ñ ÑÑÐ½Ð³Ð¸ÑÐ¸Ð´Ð½Ð¾Ð³Ð¾ Ð±Ð¸Ð¾Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: The study of collectible strains of microorganisms for the creation of a fungicidal biological product
Creators: name: ÐÐ»ÑÑÐºÐ¾Ð²Ð°, ÐÑÐµÐ½Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÑÑÐ½Ð³Ð¸ÑÐ¸Ð´Ð½Ð°Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ
ÑÐµÐ¶Ð¸Ð¼Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ
ÑÐ¸ÑÐ¾Ð¿Ð°ÑÐ¾Ð³ÐµÐ½Ñ
antibiotic
phytopathogens
fungicidal activity

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¸ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ ÑÑÐ½Ð³Ð¸ÑÐ¸Ð´Ð½Ð¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÐºÐ¾Ð»Ð»ÐµÐºÑÐ¸Ð¾Ð½Ð½ÑÑ Ð¼Ð¸ÐºÑÐ¾Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð¾Ð², Ð° ÑÐ°ÐºÐ¶Ðµ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐµ ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´ÑÑÐ²Ð° Ð±Ð¸Ð¾ÑÑÐ½Ð³Ð¸ÑÐ¸Ð´Ð° Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ Ð¿ÐµÑÑÐ¿ÐµÐºÑÐ¸Ð²Ð½ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð². ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: â Ð¾Ð¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ ÑÑÐ½Ð³Ð¸ÑÐ¸Ð´Ð½ÑÑ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð²; â Ð¿Ð¾Ð´Ð¾Ð±ÑÐ°ÑÑ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ ÑÑÑÐµÐºÑÐ¸Ð²Ð½ÑÐµ ÐºÐ¾Ð¼Ð¿Ð¾Ð½ÐµÐ½ÑÑ Ð´Ð»Ñ Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½ÑÑ ÑÑÐµÐ´; â Ð¾Ð¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ Ð¾Ð¿ÑÐ¸Ð¼Ð°Ð»ÑÐ½ÑÐµ ÑÑÐ»Ð¾Ð²Ð¸Ñ ÑÐµÐ¶Ð¸Ð¼Ð¾Ð² ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð´Ð»Ñ Ð¾ÑÐ¾Ð±ÑÐ°Ð½Ð½Ð¾Ð³Ð¾ ÑÑÐ°Ð¼Ð¼Ð°; â Ð¾Ð¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾ÑÑÑ ÑÑÐ°Ð¼Ð¼Ð° Ðº Ð¿ÑÐ¾ÑÐ¸Ð²Ð¾Ð³ÑÐ¸Ð±ÐºÐ¾Ð²Ð¾Ð¼Ñ Ð²ÐµÑÐµÑÑÐ²Ñ. ÐÐ±ÑÐµÐºÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â ÐºÐ¾Ð»Ð»ÐµÐºÑÐ¸Ñ ÑÑÐ°Ð¼Ð¼Ð¾Ð² Ð¼Ð¸ÐºÑÐ¾Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð¾Ð², Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½Ð°Ñ Ð½Ð° Ð±Ð°Ð·Ðµ Ð½Ð°ÑÑÐ½Ð¾-Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´ÑÑÐ²ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¿Ð¾Ð´ÑÐ°Ð·Ð´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¤ÐÐÐÐ£ Â«ÐÐÐÐÐ¡Ð¥ÐÂ»: Paenibacillus ÑÑ. P-411; Flavobacterium ÑÑ. Ð-30; Bacillus subtilis ÑÑ. S-28, 66; B. amyloliquefaciens ÑÑ. E-161, S21; Pseudomonas ÑÑ. ÐÐ-5, 2Ð-5, Ð-12, 2Ð-7, Ð° ÑÐ°ÐºÐ¶Ðµ Â ÐºÑÐ»ÑÑÑÑÑ ÑÐ¸ÑÐ¾Ð¿Ð°ÑÐ¾Ð³ÐµÐ½Ð½ÑÑ Ð³ÑÐ¸Ð±Ð¾Ð²: Alternaria radicina, Fusarium culmorum, Cladosporium cladosporioides, Fusarium sporotrichoides, Botrytis cinerea. Ð Ð°Ð±Ð¾ÑÐ° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ Ð½Ð°ÑÑÐ½Ð¾-Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´ÑÑÐ²ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¿Ð¾Ð´ÑÐ°Ð·Ð´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¤ÐÐÐÐ£ Â«ÐÐÐÐÐ¡Ð¥ÐÂ». Ð ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð½Ð°Ð¸Ð±Ð¾Ð»ÑÑÐ¸Ð¼ ÑÐ¿ÐµÐºÑÑÐ¾Ð¼ ÑÑÐ½Ð³Ð¸ÑÐ¸Ð´Ð½Ð¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÑÑÐµÐ´Ð¸ Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼ÑÑ Ð¼Ð¸ÐºÑÐ¾Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð¾Ð² Ð¾Ð±Ð»Ð°Ð´Ð°ÐµÑ B. amyloliquefaciens ÑÑ. S21, Ð´Ð»Ñ Ð¾ÑÐ¾Ð±ÑÐ°Ð½Ð½Ð¾Ð³Ð¾ ÑÑÐ°Ð¼Ð¼Ð° Ð¿Ð¾Ð´Ð¾Ð±ÑÐ°Ð½Ð° Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½Ð°Ñ ÑÑÐµÐ´Ð°, Ð½Ð° ÐºÐ¾ÑÐ¾ÑÐ¾Ð¹ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð° ÑÐ°Ð¼Ð°Ñ Ð·Ð½Ð°ÑÐ¸ÑÐµÐ»ÑÐ½Ð°Ñ Ð¿ÑÐ¸Ð±Ð°Ð²ÐºÐ° ÑÐ¸ÑÑÐ° â 550 106 ÐÐÐ Ð½Ð° 1 Ð¼Ð», Ð±ÑÐ» Ð¿ÑÐ¾Ð¸Ð·Ð²ÐµÐ´ÐµÐ½ Ð±Ð¸Ð¾Ð¿ÑÐµÐ¿Ð°ÑÐ°Ñ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ Ð¾ÑÐ¾Ð±ÑÐ°Ð½Ð½Ð¾Ð³Ð¾ ÑÑÐ°Ð¼Ð¼Ð° Ð½Ð° ÑÐ¾ÑÑÐ°Ð²Ð»ÐµÐ½Ð½Ð¾Ð¹ ÑÑÐµÐ´Ðµ Ñ Ð¼Ð°Ð»ÑÑÐ¾Ð·Ð½Ð¾Ð¹ Ð¿Ð°ÑÐ¾ÐºÐ¾Ð¹, Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð° ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾ÑÑÑ Ð¿ÐµÑÑÐ¿ÐµÐºÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÑÐ°Ð¼Ð¼Ð° Ðº Ð¿ÑÐ¾ÑÐ¸Ð²Ð¾Ð³ÑÐ¸Ð±ÐºÐ¾Ð²Ð¾Ð²Ð¼Ñ Ð²ÐµÑÐµÑÑÐ²Ñ ÐÐ¼ÑÐ¾ÑÐµÑÐ¸ÑÐ¸Ð½ B.
description: This work is devoted to the study and comparison of the fungicidal activity of collection microorganisms, as well as the development of biofungicide production technology based on promising strains.T he tasks of the study: â to determine the fungicidal activity of the studied strains; â to pick up the most effective components for nutrient media; â to determine the optimal conditions of cultivation modes for the selected strain; â determine resistance of the strain to the antifungal substance. Objects of research â a collection of strains of microorganisms isolated based on the scientific and production division of the ARRIAM: Paenibacillus ÑÑ. P-411; Flavobacterium ÑÑ. Ð-30; Bacillus subtilis ÑÑ. S-28, 66; B. amyloliquefaciens ÑÑ. E-161, S21; Pseudomonas ÑÑ. ÐÐ-5, 2Ð-5, Ð-12, 2Ð-7, as well as cultures of phytopathogenic fungi: Alternaria radicina, Fusarium culmorum, Cladosporium cladosporioides, Fusarium sporotrichoides, Botrytis cinerea. The work was carried out based on the scientific and production division of the ARRIAM. During the research, it was shown that B. amyloliquefaciens S21 has the greatest spectrum of fungicidal activity among the studied microorganisms, a nutrient medium was selected for the selected strain, on which the most significant titer increase was obtained â 550 106 CFU per 1 ml, a biopreparation was produced based on the selected strain on a compound medium with maltose molasses, the stability of a promising strain was determined to the antifungal substance Amphotericin B.