title: ÐÑÐµÐ½ÐºÐ° ÑÑÐ¾Ð²Ð½Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÐºÐ°Ð»ÑÑÐ¸Ð¹-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼ÑÑ ÐºÐ°Ð»Ð¸ÐµÐ²ÑÑ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² Ð² ÑÑÐ°Ð½ÑÑÐ¾ÑÐ¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ ÐºÑÐ¾Ð²Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Estimation the activity level of calcium-dependent potassium channels in trans-formed blood cells
Creators: name: Ð¥Ð°Ð¹ÑÑÐ»Ð»Ð¸Ð½Ð°, ÐÑÐ»ÐµÐ¹ÑÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: Ð¸Ð¾Ð½Ð½ÑÐµ ÐºÐ°Ð½Ð°Ð»Ñ
ÐºÐ°Ð»ÑÑÐ¸ÐµÐ²Ð°Ñ ÑÐ¸Ð³Ð½Ð°Ð»Ð¸Ð·Ð°ÑÐ¸Ñ
ÐºÐ°Ð»ÑÑÐ¸Ð¹-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼ÑÐµ ÐºÐ°Ð»Ð¸ÐµÐ²ÑÐµ ÐºÐ°Ð½Ð°Ð»Ñ
Ð¼ÐµÑ Ð°Ð½Ð¾ÑÑÐ²ÑÑÐ²Ð¸ÑÐµÐ»ÑÐ½ÑÐµ ÐºÐ°Ð½Ð°Ð»Ñ
ÐºÐ»ÐµÑÐºÐ¸ Ð¼Ð¸ÐµÐ»Ð¾Ð¸Ð´Ð½Ð¾Ð¹ Ð»ÐµÐ¹ÐºÐµÐ¼Ð¸Ð¸ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð562
Ð¼ÐµÑÐ¾Ð´ Ð»Ð¾ÐºÐ°Ð»ÑÐ½Ð¾Ð¹ ÑÐ¸ÐºÑÐ°ÑÐ¸Ð¸ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð° (Ð¿Ð°ÑÑ-ÐºÐ»Ð°Ð¼Ð¿)
ion channels
calcium signaling
calcium-dependent potassium channels
mechanosensitive channels
human myeloid leukemia K562 cells
local potential fixation method (patch clamp)

description: Ð Ð°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð²ÑÑÐ²Ð»ÐµÐ½Ð¸Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÐºÐ°Ð»ÑÑÐ¸Ð¹-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼ÑÑ ÐºÐ°Ð»Ð¸ÐµÐ²ÑÑ (KCa) ÐºÐ°Ð½Ð°Ð»Ð¾Ð² Ð¼Ð°Ð»Ð¾Ð¹ (SK) Ð¸ ÑÑÐµÐ´Ð½ÐµÐ¹ (IK) Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð¼Ð¾ÑÑÐ¸ Ð¸ Ð¾ÑÐµÐ½ÐºÐµ Ð¸Ñ Ð²ÐºÐ»Ð°Ð´Ð° Ð² ÐºÐ°Ð»Ð¸ÐµÐ²ÑÐ¹ ÑÑÐ°Ð½ÑÐ¿Ð¾ÑÑ Ð² ÐºÐ»ÐµÑÐºÐ°Ñ Ð¼Ð¸ÐµÐ»Ð¾Ð¸Ð´Ð½Ð¾Ð¹ Ð»ÐµÐ¹ÐºÐµÐ¼Ð¸Ð¸ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð»Ð¸Ð½Ð¸Ð¸ Ð562. Ð¢ÐµÐ¼Ð°ÑÐ¸ÐºÐ° Ð½Ð°ÑÑÐ½Ð¾Ð³Ð¾ Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð¸Ñ ÑÐ²Ð»ÑÐµÑÑÑ Ð²Ð°Ð¶Ð½Ð¾Ð¹ Ð² ÑÐ°Ð¼ÐºÐ°Ñ Ð²Ð¾Ð¿ÑÐ¾ÑÐ° Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½ÑÑ Ð¿Ð°ÑÑÐ½ÐµÑÐ¾Ð² Ð¼ÐµÑÐ°Ð½Ð¾ÑÑÐ²ÑÑÐ²Ð¸ÑÐµÐ»ÑÐ½ÑÑ ÐºÐ°Ð»ÑÑÐ¸Ð¹-Ð¿ÑÐ¾Ð²Ð¾Ð´ÑÑÐ¸Ñ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² Ð² ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ°Ñ Ñ KCa ÐºÐ°Ð½Ð°Ð»Ð°Ð¼Ð¸, Ð° ÑÐ°ÐºÐ¶Ðµ Ð´Ð»Ñ Ð²ÑÑÐ²Ð»ÐµÐ½Ð¸Ñ Ð½Ð¾Ð²ÑÑ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»ÑÐ½ÑÑ Ð¼Ð¸ÑÐµÐ½ÐµÐ¹ Ð´Ð»Ñ ÑÐµÐ»ÐµÐºÑÐ¸Ð²Ð½Ð¾Ð¹ Ð¾Ð½ÐºÐ¾ÑÐµÑÐ°Ð¿Ð¸Ð¸. Ð¡ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¸Ð¼Ð¼ÑÐ½Ð¾ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð³Ð¾ Ð¾ÐºÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð° Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð° ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ KCa ÐºÐ°Ð½Ð°Ð»Ð¾Ð² IK, SK2, SK3. ÐÐ´ÐµÐ½ÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ñ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾Ð¹ Ð¿ÑÐ¸Ð½Ð°Ð´Ð»ÐµÐ¶Ð½Ð¾ÑÑÐ¸ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»Ð¸ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÑÐµÐ»ÐµÐºÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾ÑÐ½Ð¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð°. ÐÑÐ½Ð¾Ð²Ð½Ð¾Ð¹ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÐ¹ Ð¿Ð¾Ð´ÑÐ¾Ð´ - Ð¼ÐµÑÐ¾Ð´ Ð¿Ð°ÑÑ-ÐºÐ»Ð°Ð¼Ð¿ Ð² ÐºÐ¾Ð½ÑÐ¸Ð³ÑÑÐ°ÑÐ¸Ð¸ whole-cell (ÑÐµÐ³Ð¸ÑÑÑÐ°ÑÐ¸Ñ Ð¸Ð½ÑÐµÐ³ÑÐ°Ð»ÑÐ½ÑÑ ÑÐ¾ÐºÐ¾Ð² Ð¾Ñ Ð²ÑÐµÐ¹ ÐºÐ»ÐµÑÐºÐ¸). ÐÐ»Ñ Ð¾ÑÐµÐ½ÐºÐ¸ ÑÑÐ¾Ð²Ð½Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ SK Ð¸ IK ÐºÐ°Ð½Ð°Ð»Ð¾Ð² Ð±ÑÐ»Ð¸ Ð·Ð°Ð¿Ð¸ÑÐ°Ð½Ñ ÑÐ¾ÐºÐ¸ Ð² ÐºÐ¾Ð½ÑÑÐ¾Ð»Ðµ (Ð´Ð¾ Ð¿Ð¾Ð´Ð°ÑÐ¸ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾ÑÐ¾Ð²) Ð¸ Ð¿Ð¾ÑÐ»Ðµ Ð¸Ñ Ð´Ð¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸Ñ Ð²Ð¾ Ð²Ð½ÐµÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐ¹ ÑÐ°ÑÑÐ²Ð¾Ñ. ÐÐ°Ð»ÐµÐµ, Ð´Ð»Ñ Ð¾ÑÐµÐ½ÐºÐ¸ Ð²ÐºÐ»Ð°Ð´Ð° SK Ð¸ IK ÐºÐ°Ð½Ð°Ð»Ð¾Ð² Ð² ÐºÐ°Ð»Ð¸ÐµÐ²ÑÐµ ÑÐ¾ÐºÐ¸ Ð±ÑÐ»Ð¸ ÑÐ°ÑÑÑÐ¸ÑÐ°Ð½Ñ ÑÑÐµÐ´Ð½Ð¸Ðµ Ð·Ð½Ð°ÑÐµÐ½Ð¸Ñ Ð¸Ð½ÑÐµÐ³ÑÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÐ¾ÐºÐ° Ð´Ð¾ Ð¸ Ð¿Ð¾ÑÐ»Ðµ Ð´Ð¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸Ñ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾ÑÐ¾Ð². ÐÑÑÐ²Ð»ÐµÐ½Ð¾, ÑÑÐ¾ ÑÑÐµÐ´Ð½Ð¸Ð¹ Ð²ÐºÐ»Ð°Ð´ ÐºÐ°Ð»Ð¸ÐµÐ²ÑÑ ÑÐ¾ÐºÐ¾Ð², Ð¿ÐµÑÐµÐ½Ð¾ÑÐ¸Ð¼ÑÑ ÐºÐ°Ð½Ð°Ð»Ð°Ð¼Ð¸ SK, ÑÐ¾ÑÑÐ°Ð²Ð»ÑÐµÑ 42,68 Â± 5,59 %, Ð° ÑÑÐ¼Ð¼Ð°ÑÐ½ÑÐ¹ Ð²ÐºÐ»Ð°Ð´ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² SK Ð¸ IK - 71,10 Â± 9,96 % Ð¾Ñ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½ÑÑ Ð·Ð½Ð°ÑÐµÐ½Ð¸Ð¹ ÐºÐ°Ð»Ð¸ÐµÐ²Ð¾Ð³Ð¾ ÑÐ¾ÐºÐ°. Ð ÐµÐ·ÑÐ»ÑÑÐ°ÑÑ ÑÐ²Ð¸Ð´ÐµÑÐµÐ»ÑÑÑÐ²ÑÐµÑ Ð¾ ÑÐ¾Ð¼, ÑÑÐ¾ Ð¿Ð¾Ð²ÑÑÐµÐ½Ð¸Ðµ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ ÐºÐ°Ð»ÑÑÐ¸Ñ Ð¼Ð¾Ð¶ÐµÑ ÑÑÐ¸Ð¼ÑÐ»Ð¸ÑÐ¾Ð²Ð°ÑÑ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð½ÐµÑÐºÐ¾Ð»ÑÐºÐ¸Ñ ÑÐ¸Ð¿Ð¾Ð² KCa ÐºÐ°Ð½Ð°Ð»Ð¾Ð², ÐºÐ¾ÑÐ¾ÑÑÐµ Ð²Ð½Ð¾ÑÑÑ Ð·Ð½Ð°ÑÐ¸ÑÐµÐ»ÑÐ½ÑÐ¹ Ð²ÐºÐ»Ð°Ð´ Ð² Ð¸Ð½ÑÐµÐ³ÑÐ°Ð»ÑÐ½ÑÐµ ÐºÐ°Ð»Ð¸ÐµÐ²ÑÐµ ÑÐ¾ÐºÐ¸ Ð² ÐºÐ»ÐµÑÐºÐ°Ñ Ð562.
description: The work is devoted to the identification of the activity of calcium-dependent potassium (KCa) channels of low (SK) and medium (IK) conductivity and the estimation of their contribution to potassium transport in human myeloid leukemia K562 cells. The research area is important in terms of determining the molecular partners of mechanosensitive calcium-permeable channels in complexes with KCa channels, as well as for identifying new potential targets for selective oncotherapy. Immunofluorescence staining showed the expression of KCa channels IK, SK2, SK3. The identification of the molecular affiliation of the channels was carried out using selective inhibitor analysis. The main experimental approach is the patch-clamp method in the whole-cell configuration (registration of integral currents from the whole cell). For level assessment of activity of SK and IK channels, currents were recorded in the control (before the supply of inhibitors) and after they were added to the extracellular solution. Further, to assess the contribution of SK and IK channels to potassium currents, the average values of the integrated current were calculated before and after the addition of inhibitors. It was found that the average contribution of the potassium currents which are carried by the SK channels is 42.68 Â± 5.59%, and the total contribution of the SK and IK channels is 71.10 Â± 9.96% of the control values of the potassium current. The results indicate the increase of the intracellular calcium concentration can stimulate the several types activity of KCa channels, which make a significant contribution to the integral potassium currents in K562 cells.