title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ Ð¼ÐµÑÑÐ½ÑÑ Ð°Ð½ÐµÑÑÐµÑÐ¸ÐºÐ¾Ð² Ð½Ð° ÐºÐ°Ð½Ð°Ð»Ð¾Ð¾Ð±ÑÐ°Ð·ÑÑÑÑÑ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÑÐºÐ·Ð¾Ð³ÐµÐ½Ð½ÑÑ ÑÐ¾ÐµÐ´Ð¸Ð½ÐµÐ½Ð¸Ð¹
title: Ð¼Ð°Ð³Ð¸ÑÑÐµÑÑÐºÐ°Ñ Ð´Ð¸ÑÑÐµÑÑÐ°ÑÐ¸Ñ
titleType: Subtitle
Creators: name: ÐÐµÐ´Ð²ÐµÐ´ÐµÐ², Ð Ð¾Ð¼Ð°Ð½
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2016
Subjects: ÐÐ¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ñ
ÐÐ¸Ð¿Ð¸Ð´Ñ
Ð¼ÐµÑÑÐ½ÑÐµ Ð°Ð½ÐµÑÑÐµÑÐ¸ÐºÐ¸
Ð·Ð¾Ð»Ð¾ÑÐ¸ÑÑÑÐ¹ ÑÑÐ°ÑÐ¸Ð»Ð¾ÐºÐ¾ÐºÐº
local anesthetics
staphylococcus aureus

description: ÐÐ¾Ð»ÐµÐºÑÐ»ÑÑÐ½ÑÐµ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ñ Ð¿ÑÐ¸Ð²Ð¾Ð´ÑÑÐ¸Ðµ Ðº ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ñ Ð¼ÐµÑÑÐ½Ð¾Ð¹ Ð°Ð½ÐµÑÑÐµÐ·Ð¸Ð¸ Ð¸ Ð¿Ð¾Ð±Ð¾ÑÐ½ÑÑ ÑÑÑÐµÐºÑÐ¾Ð² Ð¾Ñ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð¼ÐµÑÑÐ½ÑÑ Ð°Ð½ÐµÑÑÐµÑÐ¸ÐºÐ¾Ð² Ð¾ÑÑÐ°ÑÑÑÑ Ð´Ð¾ ÐºÐ¾Ð½ÑÐ° Ð½Ðµ Ð²ÑÑÑÐ½ÐµÐ½Ð½ÑÐ¼Ð¸. Ð¦ÐµÐ»ÑÑ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÐ»Ð¾ÑÑ Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð¾Ð² Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð¼ÐµÑÑÐ½ÑÑ Ð°Ð½ÐµÑÑÐµÑÐ¸ÐºÐ¾Ð² Ð½Ð° ÑÐ¸Ð·Ð¸ÐºÐ¾-ÑÐ¸Ð¼Ð¸ÑÐµÑÐºÐ¸Ðµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° Ð»Ð¸Ð¿Ð¸Ð´Ð½ÑÑ Ð¼ÐµÐ¼Ð±ÑÐ°Ð½ Ð¸ ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð¸Ð¾Ð½Ð½ÑÑ ÐºÐ°Ð½Ð°Ð»Ð¾Ð², Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð½ÑÑ ÑÐ¾ÐºÑÐ¸Ð½Ð¾Ð¼ Ð·Ð¾Ð»Ð¾ÑÐ¸ÑÑÐ¾Ð³Ð¾ ÑÑÐ°ÑÐ¸Ð»Ð¾ÐºÐ¾ÐºÐºÐ° Ð°Ð»ÑÑÐ°-Ð³ÐµÐ¼Ð¾Ð»Ð¸Ð·Ð¸Ð½Ð¾Ð¼ Ð¸ Ð°Ð½ÑÐ¸Ð¼Ð¸ÐºÑÐ¾Ð±Ð½ÑÐ¼ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð¾Ð¼ ÑÐµÐºÑÐ¾Ð¿Ð¸Ð½Ð¾Ð¼ Ð Ð² Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ð°Ñ. Ð ÑÐ°Ð±Ð¾ÑÐµ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ Ð¼ÐµÑÐ¾Ð´Ñ Ð¸Ð·Ð¼ÐµÑÐµÐ½Ð¸Ñ ÑÑÐµÑÐºÐ¸ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð¾Ð³Ð¾ Ð¼Ð°ÑÐºÐµÑÐ°, ÐºÐ°Ð»ÑÑÐµÐ¸Ð½Ð°, Ð¸Ð· Ð±Ð¾Ð»ÑÑÐ¸Ñ Ð»Ð¸Ð¿Ð¾ÑÐ¾Ð¼ Ð¸ Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ ÑÐ¾ÐºÐ¾Ð², Ð¿ÑÐ¾ÑÐµÐºÐ°ÑÑÐ¸Ñ ÑÐµÑÐµÐ· Ð¸Ð¾Ð½Ð½ÑÐµ ÐºÐ°Ð½Ð°Ð»Ñ, ÑÐµÐºÐ¾Ð½ÑÑÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐµ Ð² Ð¿Ð»Ð¾ÑÐºÐ¸Ðµ Ð»Ð¸Ð¿Ð¸Ð´Ð½ÑÐµ Ð±Ð¸ÑÐ»Ð¾Ð¸. ÐÑÐ»Ð¾ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¾, ÑÑÐ¾ Ð¼ÐµÑÑÐ½ÑÐµ Ð°Ð½ÐµÑÑÐµÑÐ¸ÐºÐ¸ ÑÐ²ÐµÐ»Ð¸ÑÐ¸Ð²Ð°ÑÑ Ð¿ÑÐ¾Ð½Ð¸ÑÐ°ÐµÐ¼Ð¾ÑÑÑ Ð»Ð¸Ð¿Ð¾ÑÐ¾Ð¼, Ð½Ðµ Ð¸Ð·Ð¼ÐµÐ½ÑÑÑ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸Ðº Ð°Ð»ÑÑÐ°-Ð³ÐµÐ¼Ð¾Ð»Ð¸Ð·Ð¸Ð½Ð¾Ð²Ð¾Ð¹ Ð¿Ð¾ÑÑ Ð¸ ÑÐ½Ð¸Ð¶Ð°ÑÑ ÐºÐ°Ð½Ð°Ð»Ð¾Ð¾Ð±ÑÐ°Ð·ÑÑÑÑÑ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÑÐµÐºÑÐ¾Ð¿Ð¸Ð½Ð° Ð Ð² Ð»Ð¸Ð¿Ð¸Ð´Ð½ÑÑ Ð±Ð¸ÑÐ»Ð¾ÑÑ. ÐÐ½Ð°Ð»Ð¸Ð· Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¿Ð¾Ð·Ð²Ð¾Ð»Ð¸Ð» Ð²ÑÑÐ²Ð¸ÑÑ, ÑÑÐ¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð°Ð½ÐµÑÑÐµÑÐ¸ÐºÐ¾Ð² Ð½Ð° Ð¼ÐµÑÐ°Ð½Ð¸ÑÐµÑÐºÐ¸Ðµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ñ Ð·Ð°Ð²Ð¸ÑÐ¸Ñ Ð¾Ñ Ð³Ð¸Ð´ÑÐ¾ÑÐ¾Ð±Ð½Ð¾ÑÑÐ¸ Ð¼Ð¾Ð»ÐµÐºÑÐ»Ñ. Ð¡Ð½Ð¸Ð¶ÐµÐ½Ð¸Ðµ Ð¼ÑÐ»ÑÑÐ¸ÐºÐ°Ð½Ð°Ð»ÑÐ½Ð¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÑÐµÐºÑÐ¾Ð¿Ð¸Ð½Ð° Ð Ð¿ÑÐ¸ Ð´Ð¾Ð±Ð°Ð²ÐºÐµ ÑÐµÑÑÐ°ÐºÐ°Ð¸Ð½Ð°, Ð±ÐµÐ½Ð·Ð¾ÐºÐ°Ð¸Ð½Ð° Ð¸ Ð±ÑÐ¿Ð¸Ð²Ð°ÐºÐ°Ð¸Ð½Ð° Ð¿ÑÐ¾Ð¸ÑÑÐ¾Ð´Ð¸Ñ Ð¿ÑÐ¸ Ð¾Ð´Ð½Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð¼ ÑÐ¼ÐµÐ½ÑÑÐµÐ½Ð¸Ð¸ ÑÑÐµÐ´Ð½ÐµÐ³Ð¾ ÑÐ¾ÐºÐ° Ð¾Ð´Ð¸Ð½Ð¾ÑÐ½ÑÑ ÐºÐ°Ð½Ð°Ð»Ð¾Ð² ÑÐµÐºÑÐ¾Ð¿Ð¸Ð½Ð° Ð Ð¸ Ð¸Ñ ÑÐ°Ð²Ð½Ð¾Ð²ÐµÑÐ½Ð¾Ð³Ð¾ ÑÐ¸ÑÐ»Ð° Ð² Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ðµ.
description: The molecular mechanisms of local anesthesia and the side effects of anesthetics usage are still remains unclear. The work is devoted to examination the effect of local anesthetics of physicochemical properties of lipid membranes and ion channel functioning, obtained from Staphilococcus aureus toxin alpha-hemolysin and antimicrobial peptide cecropin A. Methods of determination of the fluorescent marker, calcein, release from the large unilamellar liposomes and registration of currents through ion channels reconstructed in planar lipid bilayers were used. It was determined that local anesthetics increase liposome permeability, have no effect on the properties of alpha-hemolysin channel and reduce the channel-forming activity of cecropin A in the lipid bilayers. The obtained results allowed to reveal the dependence of local anesthetics action on mechanical properties of the membranes from the hydrophobisity of the molecule. The decrease in the multichannel activity of cecropin A at the introduction of tetracaine, benzoaine and bupivacaine caused by the reduction in the mean conductance of single cecropin A channels and steady state number of their channels.