Additional file 1 of A novel polymer enabled by polymerized small molecule strategy for tumor photothermal and photodynamic therapy
Creators: name: Xie, Xin
name: Wang, Ke
name: Zeng, Jie
name: Xu, Miao-Yan
name: Qu, Xin-Hui
name: Xiang, Zheng-Bin
name: Tou, Fang-Fang
name: Huang, Shaorong
name: Han, Xiao-Jian
Publisher: name: figshare
Year: 2023
Subjects: subject: Biophysics
subject: Biochemistry
subject: Space Science
subject: Physical Sciences not elsewhere classified
subject: Medicine
subject: Cell Biology
subject: Molecular Biology
subject: Physiology
subject: FOS: Biological sciences
subject: Pharmacology
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Cancer

description: Additional file 1: Figure S1. 1H NMR spectrum of PYT in CDCl3. Figure S2. GPC spectra of PYT in TCB, estimated by high temperature gel permeation chromatography with 150 °C. Figure S3. Diameters of PYT NPs after storage in a water, b acidic PBS, c PBS and d serum for different times. Figure S4. The temperature changes of PYT NPs (50 μg mL−1) were recorded under 808 nm laser irradiation with different laser powers. Figure S5. UV–Vis-NIR spectra of PYT NPs during continuous 808 nm laser irradiation (1.0 W cm−2) for 0, 3,6, 9 min, respectively. Figure S6. Time-related ROS generation of ICG mixed with DPBF under laser power density (808 nm laser, 1.0 W cm−2). Figure S7. The photographs of PYT NPs (right) and ICG (left) before and after five repeated ON/OFF cycles irradiation with 808 nm lasers (1.0 W cm−2), respectively. Figure S8. Cellular uptake efficiency of FITC-labeled PYT NPs in 4T1 cells. Scale bar: 100 μm. Figure S9. Cell viability of a 4T1, b B16F10 and c HeLa after treatment with various concentrations of PYT NPs under dark condition for 48 h. “ns” means “no significance”, one-way ANOVA. Figure S10. Cell viability of a H9c2, b NIH 3T3 and c HEK 293 T after treatment with various concentrations of PYT NPs under dark condition for 48 h. n = 4, “ns” means “no significance”, one-way ANOVA. Figure S11. Cell viability of a 4T1, b HeLa and c B16F10 after treatment with different laser power values (808 nm). n = 4, “ns” means “no significance”, P-value: ****P &lt; 0.0001, one-way ANOVA. Figure S12. Live/dead staining of 4T1 cancer cells after different treatments. Scale bar: 100 μm. Figure S13. Live/dead staining of Hela cancer cells after different treatments. Scale bar: 100 μm. Figure S14. Live/dead staining of B16F10 cancer cells after different treatments. Scale bar: 100 μm. Figure S15. In vitro wound scratch assay of 4T1 cells with different treatments. Scale bar: 100 μm. Figure S16. Expression of Bcl-2 and Bax in 4T1 cells incubated with PBS or PYT NPS (50 μg mL−1 for PYT NPs) was detected by western blot analysis. β-actin was used as the internal control. Figure S17. a Hemolytic effect of PYT NPs at different concentrations on RBCs from BALB/c mice, water group was used as the positive control, and PBS as the negative control, respectively. b Representative photos of hemolysis experiment results. n = 3, “ns” means “no significance”, P-value: ****P &lt; 0.0001. Figure S18. a Infrared images were collected from 4T1 tumor-bearing BALB/c mice at different time intervals after 808 nm laser irradiation. b Taking temperature as the ordinate and time as the abscissa, the temperature curve of laser-heated mouse tumor site was drawn. n = 3, ****P &lt; 0.0001, one-way ANOVA. Figure S19. Representative pictures of photothermal therapy for 4T1 tumor bearing mice after different treatments (PBS, NIR, PYT NPs and PYT NPs + NIR) in vivo. Table S1. Hepatic function indexes (ALB, ALT and AST) and renal function markers (UREA, CREA and UA) of mice at day 12 after treatment. Table S2. Routine blood indexes of mice at day 12 after treatment.