title: Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° ÑÐµÑ Ð½Ð¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ð¾Ð² Ð¸Ð· Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ð¸ Chlorella sorokiniana Ð¸ ÑÐ¿Ð¾ÑÐ¾Ð±Ð° Ð¸Ñ Ð´Ð¾ÑÑÐ°Ð²ÐºÐ¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Development of technology of obtaining carotenoids from microalgae Chlorella sorokiniana biomass and means of their targeted delivery
Creators: name: Ð¨Ð»ÑÐºÐ¾Ð²Ð°, ÐÐ½ÑÐ¾Ð½Ð¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð½Ð¾Ðµ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ð¸
Ð±Ð¸Ð¾Ð´Ð¾ÑÑÑÐ¿Ð½ÑÐµ ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ñ
Ð¼Ð¸ÐºÑÐ¾ÐºÐ°Ð¿ÑÑÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
directed cultivation
microalgae
bioavailable carotenoids
microencapsulation

description: Ð¦ÐµÐ»Ñ ÑÐ°Ð±Ð¾ÑÑ â ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°ÑÑ ÑÐµÐ¶Ð¸Ð¼Ñ Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð½Ð¾Ð³Ð¾ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ C. sorokiniana Ð´Ð»Ñ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ñ Ð²ÑÑÐ¾ÐºÐ¸Ð¼ ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸ÐµÐ¼ ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ð¾Ð², Ð¾Ð±Ð¾ÑÐ½Ð¾Ð²Ð°ÑÑ ÑÐ¿Ð¾ÑÐ¾Ð±Ñ Ð¿Ð¾Ð²ÑÑÐµÐ½Ð¸Ñ Ð¸Ñ Ð±Ð¸Ð¾Ð´Ð¾ÑÑÑÐ¿Ð½Ð¾ÑÑÐ¸ Ð¸ Ð°Ð´ÑÐµÑÐ½Ð¾Ð¹ Ð´Ð¾ÑÑÐ°Ð²ÐºÐ¸ Ð´Ð»Ñ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð² ÑÐ¾ÑÑÐ°Ð²Ðµ ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð°Ð»ÑÐ½ÑÑ Ð¿ÑÐ¾Ð´ÑÐºÑÐ¾Ð² Ð¿Ð¸ÑÐ°Ð½Ð¸Ñ. Ð ÑÐ°Ð¼ÐºÐ°Ñ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð½ÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ Ð¸Ð·ÑÑÐµÐ½Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ ÑÑÑÐµÑÑÐ¾Ð²Ð¾Ð³Ð¾ ÑÐ°ÐºÑÐ¾ÑÐ° (Ð¿ÐµÑÐµÐºÐ¸ÑÐ¸ Ð²Ð¾Ð´Ð¾ÑÐ¾Ð´Ð°) Ð¸ ÑÑÐ¸Ð¼ÑÐ»ÑÑÐ¾ÑÐ° ÑÐ¾ÑÑÐ° (Ð¿Ð¸ÑÐ¸Ð´Ð¾ÐºÑÐ¸Ð½Ð° â Ð²Ð¸ÑÐ°Ð¼Ð¸Ð½Ð° Ð6) Ð½Ð° Ð½Ð°ÐºÐ¾Ð¿Ð»ÐµÐ½Ð¸Ðµ Ð² Ð±Ð¸Ð¾Ð¼Ð°ÑÑÐµ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ð¸ C. sorokiniana ÑÐ¾ÑÐ¾ÑÐ¸Ð½ÑÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð¿Ð¸Ð³Ð¼ÐµÐ½ÑÐ¾Ð² Ð² Ð¿ÑÐ¾ÑÐµÑÑÐµ Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð½Ð¾Ð³Ð¾ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ. Ð£ÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½ Ð´Ð¾Ð¿ÑÑÑÐ¸Ð¼ÑÐ¹ ÑÑÐ¾Ð²ÐµÐ½Ñ Ð¸ ÑÐµÐ¶Ð¸Ð¼ Ð²Ð½ÐµÑÐµÐ½Ð¸Ñ Ð´Ð¾Ð±Ð°Ð²Ð¾Ðº Ð¿ÐµÑÐµÐºÐ¸ÑÐ¸ Ð²Ð¾Ð´Ð¾ÑÐ¾Ð´Ð° Ð² ÐºÑÐ»ÑÑÑÑÐ°Ð»ÑÐ½ÑÑ ÑÑÐµÐ´Ñ Ð´Ð»Ñ ÑÐ¾Ð·Ð´Ð°Ð½Ð¸Ñ ÑÑÑÐµÑÑÐ¾Ð²ÑÑ ÑÑÐ»Ð¾Ð²Ð¸Ð¹. ÐÑÐ¼ÐµÑÐµÐ½Ð° Ð²Ð·Ð°Ð¸Ð¼Ð¾ÑÐ²ÑÐ·Ñ Ð¼ÐµÐ¶Ð´Ñ Ð°ÐºÑÐ¸Ð²Ð½ÑÐ¼ Ð½Ð°ÐºÐ¾Ð¿Ð»ÐµÐ½Ð¸ÐµÐ¼ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð¸ Ð·Ð°ÑÐµÐ»Ð°ÑÐ¸Ð²Ð°Ð½Ð¸ÐµÐ¼ ÑÑÐµÐ´Ñ, Ð¸ Ð½Ð°Ð¾Ð±Ð¾ÑÐ¾Ñ, Ð·Ð°ÐºÐ¸ÑÐ»ÐµÐ½Ð¸ÐµÐ¼ ÑÑÐµÐ´Ñ Ð¿ÑÐ¸ Ð¿ÐµÑÐµÑÐ¾Ð´Ðµ ÑÐ¾ÑÑÐ° ÐºÑÐ»ÑÑÑÑÑ Ð² ÑÐ°Ð·Ñ ÑÑÐ°Ð±Ð¸Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸. Ð£ÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¾, ÑÑÐ¾ Ð¿ÑÐ¸ ÑÐ¾ÑÐµÑÐ°Ð½Ð½Ð¾Ð¼ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ð¸ Ð¿ÐµÑÐµÐºÐ¸ÑÐ¸ Ð²Ð¾Ð´Ð¾ÑÐ¾Ð´Ð° Ð¸ Ð¿Ð¸ÑÐ¸Ð´Ð¾ÐºÑÐ¸Ð½Ð° ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ðµ ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ð¾Ð² Ð¸ ÑÐ»Ð¾ÑÐ¾ÑÐ¸Ð»Ð»Ð¾Ð² Ð² Ð±Ð¸Ð¾Ð¼Ð°ÑÑÐµ ÑÐ²ÐµÐ»Ð¸ÑÐ¸Ð²Ð°ÐµÑÑÑ Ð¿Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ Ñ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÐµÐ¼ Ð² ÑÑÐµÐ´Ð½ÐµÐ¼ Ð² 2 ÑÐ°Ð·Ð°. ÐÐµÑÐ¾Ð´Ð¾Ð¼ ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸ Ñ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸ÐµÐ¼ Ð£Ð-Ð´ÐµÐ·Ð¸Ð½ÑÐµÐ³ÑÐ°ÑÐ¸Ð¸ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð²ÑÐ´ÐµÐ»ÐµÐ½ Ð¿Ð¸Ð³Ð¼ÐµÐ½ÑÐ½ÑÐ¹ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑ C. sorokiniana, Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ñ ÑÐ¿ÐµÐºÑÑÐ°Ð»ÑÐ½ÑÐµ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð¿Ð¸Ð³Ð¼ÐµÐ½ÑÐ¾Ð². ÐÐ¾Ð»ÑÑÐµÐ½ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°Ñ ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ð¾Ð² Ð² Ð²Ð¸Ð´Ðµ Ð¼Ð°ÑÐ»ÑÐ½Ð¾Ð³Ð¾ ÑÐºÑÑÑÐ°ÐºÑÐ°. ÐÐ±Ð¾ÑÐ½Ð¾Ð²Ð°Ð½Ð° ÑÐµÐ»ÐµÑÐ¾Ð¾Ð±ÑÐ°Ð·Ð½Ð¾ÑÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ñ Ð°Ð»ÑÐ³Ð¸Ð½Ð°ÑÐ° Ð½Ð°ÑÑÐ¸Ñ Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ð° Â«Ð¾Ð±Ð¾Ð»Ð¾ÑÐºÐ¸Â» Ð´Ð»Ñ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ Ð±Ð¸Ð¾Ð´Ð¾ÑÑÑÐ¿Ð½ÑÑ Ð¼Ð¸ÐºÑÐ¾ÐºÐ°Ð¿ÑÑÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ð¾Ð². ÐÐ¿Ð¸ÑÐ°Ð½ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½ÑÐ¹ ÑÐ¿ÐµÐºÑÑ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð¼Ð¸ÐºÑÐ¾ÐºÐ°Ð¿ÑÑÐ» Ð² ÑÐµÑÐµÐ¿ÑÑÑÐµ ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð°Ð»ÑÐ½ÑÑ Ð¿Ð¸ÑÐµÐ²ÑÑ Ð¿ÑÐ¾Ð´ÑÐºÑÐ¾Ð².
description: The given work is devoted to developing modes of directed cultivation of C. sorokiniana with the purpose of obtaining the biomass with high carotenoid complex content, substantiating ways to increase its bioavailability and means of its targeted delivery within functional nutraceuticals. During the conducted study influence of a stress factor (hydrogen peroxide) and a growth stimulator (pyridoxine â vitamin B6) on accumulation of photosynthetic pigments by C. sorokiniana microalgae cells was elucidated in the framework of directed cultivation. Maximum allowable volume and mode of hydrogen peroxide treatment were established when creating stress conditions during cultivation. Relation was revealed between active biomass accumulation and alkalization of the medium, as well as, oppositely, acidification during transition to stabilization growth phase. It was established that combined treatment with hydrogen peroxide and pyridoxine entailed ca. two-fold increase in contents of carotenoids and chlorophyll a and b, as compared to control. Extract of a pigment complex was obtained from microalgae biomass by extraction method employing ultrasound disintegration, extractâs spectral characteristics were studied. Carotenoid concentrate was obtained in the form of oil extract. Feasibility of sodium alginate utilization as a material for coating of bioavailable microencapsulated carotenoids was substantiated. Recommendations on the use of capsules in preparation of functional foods were described.