cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Bagrada funerea Horvath 1901
Creators: name: Dursun, Ahmet
name: Fent, Meral
Publisher: name: Zenodo
Year: 2017
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hemiptera
Pentatomidae
Bagrada
Bagrada funerea

description: Bagrada funerea Horváth, 1901
TL: Hamidie [= Hamidiye] (Ceyhan, Adana); 36°59'30"N, 35°59'46"E. DT: Adana (Horváth, 1901).
description: Published as part of Dursun, Ahmet & Fent, Meral, 2017, Type Localities of Heteroptera (Insecta: Hemiptera) from Turkey, pp. 451-494 in Zootaxa 4227 (4) on page 475, DOI: 10.11646/zootaxa.4227.4.1, http://zenodo.org/record/293602
MOESM2 of Where do people purchase food? A novel approach to investigating food purchasing locations
Creators: name: Lukar Thornton
name: Crawford, David
name: Lamb, Karen
name: Ball, Kylie
Publisher: name: Figshare
Year: 2017
Subjects: subject: Medicine
subject: Molecular Biology
subject: Physiology
subject: FOS: Biological sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics
subject: Computational Biology

description: Additional file 2. Supplementary tables.
Austrotinodes taquaralis Thomson & Holzenthal 2010
Creators: name: Leandro Lourenço Dumas
name: Wagner Rafael M. de Souza
Publisher: name: Zenodo
Year: 2017
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Trichoptera
Ecnomidae
Austrotinodes
Austrotinodes taquaralis

description: Austrotinodes taquaralis Thomson & Holzenthal, 2010
Austrotinodes taquaralis Thomson & Holzenthal, 2010: 47, fig. 6a–f [
Type locality: Brazil, Rio de Janeiro, Parque Nacional Itatiaia, Rio Taquaral, 22°27.252′ S, 44°36.570′ W, 1300 m; holotype depository: MZSP; male].
Austrotinodes taquaralis – Paprocki & França 2014: 7 [checklist].
Material examined
BRAZIL: 1 ♂, Espírito Santo, Dores do Rio Preto, Pedra Menina, PN do Caparaó, Cachoeira do Aurélio, 20°28′54.5″ S, 41°50′12.8″ W, 6 Jan. 2013, 1814 m, L.L. Dumas and B.H.L. Sampaio leg. (DZRJ).
Remarks
Austrotinodes taquaralis is very similar to A. donagrazielae sp. nov., A. absaberi sp. nov. and A. gusmaoi sp. nov., possibly forming a species group or complex. There are minor differences among then, as mentioned in the species diagnoses of those species. This species can be diagnosed by a set of characteristics, including intermediate appendages tapering to apex, with a single basodorsal and 3 apical spine-like setae; inferior appendages with posterior margin concave with central point; and a phallus with the lateral process bearing a short slender ventral branch with a single apical spine, and a dorsal branch with a row of 2 subapical and 1 apical unenlarged spines.
This species is commonly encountered at Atlantic Forest sites in Itatiaia, Rio de Janeiro state, and Minas Gerais. It is reported for the first time and from similar sites from Espirito Santo state.
description: Published as part of Leandro Lourenço Dumas & Wagner Rafael M. de Souza, 2017, On Brazilian Austrotinodes Schmid, 1955 (Insecta, Trichoptera, Ecnomidae): new species, new distributional records and an updated checklist, pp. 1-40 in European Journal of Taxonomy 297 on page 38, DOI: 10.5852/ejt.2017.297, http://zenodo.org/record/894203
Bagrada stolida Herrich & Schaeffer 1839
Creators: name: Dursun, Ahmet
name: Fent, Meral
Publisher: name: Zenodo
Year: 2017
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hemiptera
Pentatomidae
Bagrada
Bagrada stolida

description: Bagrada stolida (Herrich & Schaeffer, 1839)
Eurydema stolidum Herrich & Schaeffer, 1839
TL-Syntype: European part of Turkey and Greece (lost)
DT: Amasya, Ankara, Aydın, Batman, Burdur, Bursa, İzmir, Kayseri, Muğla, Mardin, Şanlıurfa, Gaziantep, Kahramanmaraş, Kırşehir, Kilis, Tokat, (Horváth, 1936; Önder et al., 2006, Dursun & Kartal, 2008a; Fent et al., 2010a).
description: Published as part of Dursun, Ahmet & Fent, Meral, 2017, Type Localities of Heteroptera (Insecta: Hemiptera) from Turkey, pp. 451-494 in Zootaxa 4227 (4) on page 475, DOI: 10.11646/zootaxa.4227.4.1, http://zenodo.org/record/293602
title: Short-term-outcomes of idiopathic epiretinal membranes treated with pars-planavitrectomy – examination of visual function and OCT-morphology
Creators: name: Englmaier, Verena Anna
name: Storp, Jens Julian
name: Eter, Nicole
name: Al-Nawaiseh, Sami
Publisher: name: University of Muenster, Institutional Repository MIAMI
Year: 2025
Subjects: subject: 610 Medicine and health

description: Background: Epiretinal membranes (ERM) represent one of the most common findings in retinal examination. Structural changes of the retinal layers in patients with ERM can be visualized and classified using OCT. The purpose of this study is to evaluate structural and functional changes related to surgical treatment of ERM. Methods: Monocentric retrospective analysis of 92 patients who underwent 23-gauge-pars plana vitrectomy (ppV) combined with cataract surgery for idiopathic ERM from 2015 to 2020. Visual acuity was determined directly preoperatively, at four weeks and three months postoperatively. Disease stage and tomographic biomarkers related to ERM were assessed in OCT imaging. Results: 92 eyes of 92 patients were included. At the time of surgery, the mean patient age was 71 years. Visual acuity improved significantly by 2 lines postoperatively, on average from LogMar 0.4 to 0.2 (p < 0.001). Disease stage regressed from stage 3 to stage 2 postoperatively (p < 0.001). No patient had stage 4 postoperatively (n = 0). In the presence of preoperative intraretinal fluid, mean retinal thickness was 488 μm and decreased to 392 μm postoperatively (n = 32; p < 0.001). Preoperative presence of a Cotton Ball Sign (n = 30) was associated with better visual acuity (p = 0.009). This was also visible in patients with preoperative vitreomacular traction syndrome (p < 0.001). The presence of preoperative intraretinal fluid showed a tendency towards better disease staging after surgery (p = 0.080). Conclusion: Surgery was able to achieve visual improvement and morphological regression of the preoperative OCT findings related to ERM. ppV led to a reduction in retinal thickness and disease stage. The presence of the Cotton Ball Sign and vitreomacular traction was associated with better visual acuity in the follow-up period. In our cohort the preoperative presence of intraretinal fluid showed a tendency for better postoperative disease staging.
description: Finanziert über die DEAL-Vereinbarung mit Wiley, Springer oder Elsevier 2019–2023.
Additional file 1 of A lifestyle screening tool for young children in the community: needs and wishes of parents and youth healthcare professionals
Creators: name: Krijger, Anne
name: Schiphof-Godart, Lieke
name: Lanting, Caren
name: Elstgeest, Liset
name: Raat, Hein
name: Joosten, Koen
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Biotechnology
subject: Sociology
subject: FOS: Sociology
subject: Biological Sciences not elsewhere classified
subject: Plant Biology
subject: FOS: Biological sciences

description: Supplementary Material 1
Additional file 2: Table S2. of Machine learning classifier for identification of damaging missense mutations exclusive to human mitochondrial DNA-encoded polypeptides
Creators: name: Martín-Navarro, Antonio
name: Gaudioso-Simón, Andrés
name: Álvarez-Jarreta, Jorge
name: Montoya, Julio
name: Mayordomo, Elvira
name: Ruiz-Pesini, Eduardo
Publisher: name: Figshare
Year: 2017
Subjects: subject: Biochemistry
subject: Space Science
subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Evolutionary Biology
subject: 69999 Biological Sciences not elsewhere classified
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: Developmental Biology

description: Domains (segments of amino acids) of mtDNA-encoded polypeptides. IM, TM and M code for intermembrane, transmembrane and matrix domains, respectively. (DOC 38 kb)
Opinions Of School Administrators And Teachers On Health Management Practices In Schools During The Covid 19 Pandemic
Creators: name: İNCE, Zuhal
Publisher: name: Zenodo
Year: 2024

description: The interruption of face-to-face education due to the Covid 19 pandemic has brought along some problems in realizing the goals of schools. In such cases, new practices should be developed and some changes should be made in order to carry out education and training activities in schools and to protect children's health. For this purpose, it is necessary to determine the current situation, identify the activities implemented in schools, eliminate uncertainties and carry out preventive studies. This study aims to reveal the opinions of school administrators and teachers on health management practices in schools during the Covid 19 pandemic period. The population of the study consists of school administrators and teachers working in some schools in the central district national education directorates of Ankara in the 2022-2023 academic year. The study group consists of eight school administrators and twelve teachers determined by maximum diversity sampling method. The research was conducted with the case study method, one of the qualitative research methods. Data were collected through semi-structured interviews and analyzed through content analysis. At the end of the research, the opinions of school administrators and teachers on health management practices in schools during the Covid 19 pandemic period were determined. Some suggestions were made based on the results.
Bagrada turcica Horvath 1936
Creators: name: Dursun, Ahmet
name: Fent, Meral
Publisher: name: Zenodo
Year: 2017
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hemiptera
Pentatomidae
Bagrada
Bagrada turcica

description: Bagrada turcica Horváth, 1936
TL: Angora [= Ankara]; 39°54'38"N, 32°57'51"E. DT: Ankara (Horváth, 1936).
description: Published as part of Dursun, Ahmet & Fent, Meral, 2017, Type Localities of Heteroptera (Insecta: Hemiptera) from Turkey, pp. 451-494 in Zootaxa 4227 (4) on page 475, DOI: 10.11646/zootaxa.4227.4.1, http://zenodo.org/record/293602
Additional file 4: of Linking families with pre-school children from healthcare services to community resources: a systematic review protocol
Creators: name: Burns, Jacky
name: Conway, David
name: Gnich, Wendy
name: Macpherson, Lorna
Publisher: name: Figshare
Year: 2017
Subjects: subject: Molecular Biology
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Science Policy

description: Data extraction form. (DOCX 14 kb)
Eurydema blanda Horvath 1903
Creators: name: Dursun, Ahmet
name: Fent, Meral
Publisher: name: Zenodo
Year: 2017
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hemiptera
Pentatomidae
Eurydema
Eurydema blanda

description: Eurydema blanda Horváth, 1903
TL: Ak-Chéhir [= Akşehir] (Konya); 38°21'40"N, 31°24'12"E.
DT: Adana, Afyonkarahisar, Amasya, Ankara, Antalya, Aydın, Balıkesir, Bilecik, Bolu, Burdur, Çanakkale, Denizli, Erzurum, Gümüşhane, Hakkari, Isparta, İzmir, Kahramanmaraş, Kars, Kastamonu, Kırıkkale, Kırklareli, Kocaeli, Konya, Kütahya, Malatya, Manisa, Niğde, Ordu, Samsun, Sivas, Şırnak, Tokat, Uşak, Yalova, (Hoberlandt, 1956: Lodos et al., 1978; Lodos et al., 1998; Dursun & Kartal, 2008b; Dursun & Fent, 2011a). Note: This species is endemic to Anatolia.
description: Published as part of Dursun, Ahmet & Fent, Meral, 2017, Type Localities of Heteroptera (Insecta: Hemiptera) from Turkey, pp. 451-494 in Zootaxa 4227 (4) on page 475, DOI: 10.11646/zootaxa.4227.4.1, http://zenodo.org/record/293602
Additional file 4: of Post-transcriptional regulation of fruit ripening and disease resistance in tomato by the vacuolar protease SlVPE3
Creators: name: Weihao Wang
name: Jianghua Cai
name: Peiwen Wang
name: Shiping Tian
name: Guozheng Qin
Publisher: name: Figshare
Year: 2017
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: Plant Biology
subject: 60506 Virology

description: Supplementary figures S1â  S6 and Supplementary text. Figure S1. Protein sequence alignment of tomato vacuolar processing enzymes (VPEs) using Clustal X. Figure S2. Prediction of the potential off-targets of the RNAi construct. Figure S3. Identification of SlVPE3 by mass spectrometry. Figure S4. Determination of KTI4 antibody specificity. Figure S5. Effects of biotin-YVAD-fmk on vacuolar processing enzyme (VPE) activity in tobacco (N. benthamiana) leaves transiently expressing SlVPE3. Figure S6. Virus-induced gene silencing (VIGS) of KTI4 in tomato has no effect on fruit ripening. Supplementary text Protein sequences of tomato vacuolar processing enzymes (VPEs). (PDF 1450Â kb)
Scripts for processing eDNA metabarcoding data to monitor temporal and spatial variations in fish communities structure in the Belgian part of the North Sea
Creators: name: Lolivier, Marianne
Publisher: name: Zenodo
Year: 2024

description: This repository contains the scripts to analyze (bioinformatics, taxonomic assignment, and data analyses) the 12S eDNA metabarcoding data used to monitor the temporal and spatial variations in fish communities structure in the coastal area of the Belgian part of the North Sea.
Eurydema caligata Horvath 1901
Creators: name: Dursun, Ahmet
name: Fent, Meral
Publisher: name: Zenodo
Year: 2017
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hemiptera
Pentatomidae
Eurydema
Eurydema caligata

description: Eurydema caligata Horváth, 1901
Eurydema fieberi var. caligatum Horváth, 1901
TL: Akbès [=Akbez], (Hassa, Hatay); 36°51'09"N, 36°33'11"E. DT: Hatay (Horváth, 1901; Rider, 2006).
Note: This species is little known and endemic to Anatolia.
description: Published as part of Dursun, Ahmet & Fent, Meral, 2017, Type Localities of Heteroptera (Insecta: Hemiptera) from Turkey, pp. 451-494 in Zootaxa 4227 (4) on page 475, DOI: 10.11646/zootaxa.4227.4.1, http://zenodo.org/record/293602
The Influence of Anthropometrics on Cardiac Mechanics in Healthy Women With Opposite Obesity Phenotypes (Android vs Gynoid)
Creators: name: Sonaglioni, Andrea
name: Ferrulli, Anna
name: Nicolosi, Gian Luigi
name: Lombardo, Michele
name: Luzi, Livio
Publisher: name: Zenodo
Year: 2024

description: Background: The possible influence exerted by mechanical factors and/or compressive phenomena on myocardial strain parameters in healthy individuals with opposite obesity phenotypes (android vs gynoid) has never been previously investigated. Accordingly, we aimed at evaluating the relationship between anthropometrics, such as the waist-to-hip ratio (WHR), modified Haller index (MHI, the ratio of chest transverse diameter over the distance between sternum and spine), and epicardial adipose tissue (EAT), and left ventricular (LV)-global longitudinal strain (GLS), in healthy women with opposite obesity phenotypes (android vs gynoid).

Methods: Forty healthy women with obesity (body mass index (BMI) ≥30 Kg/m2) and WHR ≥0.85 ("android group") (52.5±13.2 yrs), 40 age- and BMI-matched healthy women with obesityand WHR <0.78 ("gynoid group") (49.8±13.4 yrs) and 40 age-matched healthy women without obesity (BMI <30 Kg/m2) (controls) (50.3±12.5 yrs) were retrospectively analyzed. All women underwent transthoracic echocardiography implemented with echocardiographic strain analysis of all cardiac chambers. Correlation between LV-GLS and anthropometrics (WHR, MHI, and EAT) was assessed in both groups of obese women. Age, WHR, homeostasis model assessment for insulin resistance (HOMA-IR), and left ventricular mass index (LVMi) were included in the logistic regression analysis performed for evaluating the independent predictors of reduced LV-GLS magnitude (less negative than -20%) in women with android obesity.

Results: Compared to the other groups of women, those with android obesity were found with significantly greater LVMi, higher LV filling pressures, and lower biventricular and biatrial deformation indices. A strong inverse correlation between LV-GLS and all anthropometrics (WHR, MHI, and EAT) was demonstrated in both groups of women with obesity. Univariate logistic regression analysis revealed that WHR (OR 1.58, 95%CI 1.22-2.03, p<0.001) and LVMi (OR 1.09, 95%CI 1.02-1.16, p=0.006) were independently correlated with LV-GLS impairment in women with android obesity. On multivariate logistic regression analysis, the WHR maintained a statistically significant association with the above-mentioned outcome (OR 1.68, 95%CI 1.14-2.48, p=0.009). Receiver operating characteristic (ROC) curve analysis showed that a WHR value ≥1.01 had 93% sensitivity and 100% specificity for detecting LV-GLS impairment in women with android obesity (AUC=0.98; 95%CI 0.96-1.00).

Conclusions: Anthropometrics may strongly influence cardiac mechanics in healthy women with obesity. The WHR is associated with reduced LV-GLS magnitude in healthy women with android obesity, independent of age, glycometabolic status, and LV size.
title: Appendix: How to Conduct Effective Interviewer Training: A Meta-Analysis and Systematic
title: Supplementary materials for: How to Conduct Effective Interviewer Training: A Meta-Analysis and Systematic
titleType: AlternativeTitle
Creators: name: Daikeler, Jessica
name: Bosnjak, Michael
Publisher: name: PsychArchives
Year: 2020
Subjects: subject: interviewer training
subject: systematic review
subject: meta-analysis
subject: 150

description: Online Appendix for the following bookchapter: Daikeler, J. & Bosnjak, M. (2020). How to conduct effective interviewer training: A meta-analysis. In K. Olson, J. Smyth, J. Dykema, A. Holbrook, F. Kreuter and B. West (Eds.), Interviewer Effects from a Total Survey Error Perspective. Boca Raton, FL, CRC Press.
description: Appendix_Chapter4_Daikeler_Bosnjak.pdf
Additional file 3: Figure S1. of Implications of hepatitis C virus subtype 1a migration patterns for virus genetic sequencing policies in Italy
Creators: name: Cuypers, Lize
name: Vrancken, Bram
name: Fabeni, Lavinia
name: Marascio, Nadia
name: Cento, Valeria
name: Maio, Velia Di
name: Aragri, Marianna
name: Pineda-PeĂąa, Andrea
name: Schrooten, Yoeri
name: Laethem, Kristel Van
name: Balog, Daniel
name: FocĂ, Alfredo
name: Torti, Carlo
name: Nevens, Frederik
name: Perno, Carlo
name: Anne-Mieke Vandamme
name: Ceccherini-Silberstein, Francesca
Publisher: name: Figshare
Year: 2017
Subjects: subject: Microbiology
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Evolutionary Biology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: 60506 Virology

description: The thirteen highly supported Italian clusters indicated in the maximum clade credibility summary tree. The highly supported Italian-only clusters are highlighted in green, and the cluster number corresponding to the numbering in TableÂ 3 is also detailed. The red background marks the dominant Q80K clade. The evolutionary distance bar at the bottom indicates the percentage of nucleotide substitutions per site along each lineage. (PDF 50Â kb)
Additional file 1 of Long-term exposure to transportation noise and diabetes mellitus mortality: a national cohort study and updated meta-analysis
Creators: name: Vienneau, Danielle
name: Wicki, Benedikt
name: Flückiger, Benjamin
name: Schäffer, Beat
name: Wunderli, Jean Marc
name: Röösli, Martin
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Sociology
subject: FOS: Sociology
subject: Biological Sciences not elsewhere classified
subject: Science Policy

description: Supplementary Material 1.
Capsus delagrangei Puton 1892
Creators: name: Dursun, Ahmet
name: Fent, Meral
Publisher: name: Zenodo
Year: 2017
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hemiptera
Miridae
Capsus
Capsus delagrangei

description: Derula delagrangei Puton, 1892
TL: Akbès [=Akbez], (Hassa, Hatay); 36°51'09"N, 36°33'11"E. DT: Adıyaman, Diyarbakır, Gaziantep, Hatay, Kilis, Mardin (Seidenstücker, 1960a; Lodos et al., 1998; Önder et al., 2006).
description: Published as part of Dursun, Ahmet & Fent, Meral, 2017, Type Localities of Heteroptera (Insecta: Hemiptera) from Turkey, pp. 451-494 in Zootaxa 4227 (4) on page 475, DOI: 10.11646/zootaxa.4227.4.1, http://zenodo.org/record/293602
Covid-19 Pandemisi Nedeniyle Uygulanan Uzaktan Eğitime Yönelik İlköğretimde Görev Yapan Öğretmenlerin Yaşadığı Problemler Ve Hazırbulunuşluk Seviyeleri
Creators: name: PARLAK, Taşkın
name: SÜLE, Bilgi
name: BAYKAN, Hatice Efsun
name: ÇALIK, Tamer
name: EKİCİ, Osman
name: PARLAK, Dilek
name: SÜLE, Handan
Publisher: name: Zenodo
Year: 2024

description: Bu çalışmanın amacı; Kayseri ili, Melikgazi ilçesinde bulunan ilköğretim kademesinde görev yapan öğretmenlerin Covid-19 pandemisi nedeniyle uygulanan uzaktan eğitim faaliyetlerine yönelik hazırbulunuşluk seviyelerinin belirlenmesi ve yaşadıkları problemlerin tespit edilmesidir. Araştırmanın evrenini Kayseri ilinde bulunan ilköğretim okullarında görev yapan öğretmenler, örneklemini ise Kayseri ili, Melikgazi ilçesine bağlı bulunan dokuz adet ilköğretim okulunda görev yapan sınıf ve branş öğretmenleri oluşturmaktadır.

Araştırmada, literatür taraması ve anket tekniği veri toplama aracı olarak kullanılmıştır. Anket dört bölümden oluşmaktadır. Birinci bölümde öğretmenlerin kişisel özelliklerini belirlemeye yönelik hazırlanmış altı soru, ikinci bölümde öğretmenlerin uzaktan eğitim bilgi seviyelerini ölçmeye yönelik sekiz soru, üçüncü bölümde öğretmenlerin uzaktan eğitim ile ilgili tecrübelerini belirlemeye yönelik yedi soru bulunmaktadır. Dördüncü bölümde ise öğretmenlerin uzaktan eğitimde yaşadığı sorunları belirlemek için hazırlanmış olan 18 adet beş seçenekli (Hiçbir zaman, Nadiren, Bazen, Çoğu zaman, Daima) li Likert tip ölçekten faydalanılmıştır.

Elde edilen veriler istatistiksel olarak değerlendirilmiş ve yüzdelik olarak belirtilmiştir. Yapılan analizler sonucunda; öğretmenlerin %89,5’inin uzaktan eğitim konusunda hizmet içi eğitim almadığı, katılımcıların %87,5’inin ise Covid-19 pandemisi nedeniyle uygulanan uzaktan eğitim faaliyetleri öncesinde uzaktan eğitim yöntemiyle ders vermediği görülmüştür. Buna karşın öğretmenlerin %60,5’i uzaktan eğitim sistemlerine materyal yükleyebileceğini, %91,4’ü Eğitim Bilişim Ağı (EBA) kullandığını belirtmiştir. Öğretmenlerin çoğunluğunun uzaktan eğitim konusunda eğitim almamasına karşın sisteme hızlı adapte oldukları görülmektedir. Öğretmenlerin %55,3’ü uzaktan eğitimde yaşadığı en büyük problemin öğrenci kaynaklı (internete erişim,  derse katılım, ilgisizlik vb. ) olduğunu, %15,8’i uzaktan eğitim platformlarının sistem altyapı sorunlarından kaynaklandığını, %11,2’si kendi internet bağlantılarından kaynaklandığını belirtmiştir. Öğretmenlerin uzaktan eğitim sürecinde yaşanan problemlerin en büyüğünün öğrenci kaynaklı olduğu kanaatinde olmaları araştırmanın ilgi çekici sonuçlarından birisidir.
Definition and data-driven reconstruction of asymptotic phase and amplitudes of stochastic oscillators via Koopman operator theory
Creators: name: Takata, Shohei
name: Kato, Yuzuru
name: Nakao, Hiroya
Publisher: name: arXiv
Year: 2025
Subjects: subject: Adaptation and Self-Organizing Systems (nlin.AO)
subject: FOS: Physical sciences

description: Asymptotic phase and amplitudes are fundamental concepts in the analysis of limit-cycle oscillators. In this paper, we briefly review the definition of these quantities, particularly a generalization to stochastic oscillatory systems from the viewpoint of Koopman operator theory, and discuss a data-driven approach to estimate the asymptotic phase and amplitude functions from time-series data of stochastic oscillatory systems. We demonstrate that the standard Extended dynamic mode decomposition (EDMD) can successfully reconstruct the phase and amplitude functions of the noisy FitzHugh-Nagumo neuron model only from the time-series data.
description: 14 pages, 2 figures
Additional File 1:
Creators: name: Barabas, Sascha
name: Spindler, Theresa
name: Kiener, Richard
name: Tonar, Charlotte
name: Lugner, Tamara
name: Batzilla, Julia
name: Bendfeldt, Hanna
name: Rascle, Anne
name: Asbach, Benedikt
name: Wagner, Ralf
name: Deml, Ludwig
Publisher: name: Figshare
Year: 2017
Subjects: subject: Biochemistry
subject: Space Science
subject: Pharmacology
subject: Evolutionary Biology
subject: FOS: Biological sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Sociology
subject: FOS: Sociology
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics
subject: Cancer
subject: Hematology
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Virology
subject: Computational Biology

description: Assay variability. Intra-assay (Table S1.), inter-assay (Table S2.), inter-operator (Table S3.) and inter-site (Table S4.) variability was assessed as described in the respective legends. (PDF 128Â kb)
Additional file 3: Figure S1. of Implications of hepatitis C virus subtype 1a migration patterns for virus genetic sequencing policies in Italy
Creators: name: Cuypers, Lize
name: Vrancken, Bram
name: Fabeni, Lavinia
name: Marascio, Nadia
name: Cento, Valeria
name: Maio, Velia Di
name: Aragri, Marianna
name: Pineda-PeĂąa, Andrea
name: Schrooten, Yoeri
name: Laethem, Kristel Van
name: Balog, Daniel
name: FocĂ, Alfredo
name: Torti, Carlo
name: Nevens, Frederik
name: Perno, Carlo
name: Anne-Mieke Vandamme
name: Ceccherini-Silberstein, Francesca
Publisher: name: Figshare
Year: 2017
Subjects: subject: Microbiology
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Evolutionary Biology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Virology

description: The thirteen highly supported Italian clusters indicated in the maximum clade credibility summary tree. The highly supported Italian-only clusters are highlighted in green, and the cluster number corresponding to the numbering in TableÂ 3 is also detailed. The red background marks the dominant Q80K clade. The evolutionary distance bar at the bottom indicates the percentage of nucleotide substitutions per site along each lineage. (PDF 50Â kb)
Additional file 4: of Post-transcriptional regulation of fruit ripening and disease resistance in tomato by the vacuolar protease SlVPE3
Creators: name: Weihao Wang
name: Jianghua Cai
name: Peiwen Wang
name: Shiping Tian
name: Guozheng Qin
Publisher: name: Figshare
Year: 2017
Subjects: subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Plant Biology
subject: Virology

description: Supplementary figures S1â  S6 and Supplementary text. Figure S1. Protein sequence alignment of tomato vacuolar processing enzymes (VPEs) using Clustal X. Figure S2. Prediction of the potential off-targets of the RNAi construct. Figure S3. Identification of SlVPE3 by mass spectrometry. Figure S4. Determination of KTI4 antibody specificity. Figure S5. Effects of biotin-YVAD-fmk on vacuolar processing enzyme (VPE) activity in tobacco (N. benthamiana) leaves transiently expressing SlVPE3. Figure S6. Virus-induced gene silencing (VIGS) of KTI4 in tomato has no effect on fruit ripening. Supplementary text Protein sequences of tomato vacuolar processing enzymes (VPEs). (PDF 1450Â kb)
Additional file 1 of De novo assembly of the complete mitochondrial genome of pepino (Solanum muricatum) using PacBio HiFi sequencing: insights into structure, phylogenetic implications, and RNA editing
Creators: name: Li, Ziwei
name: Liu, Jiaxun
name: Liang, Mingtai
name: Guo, Yanbing
name: Chen, Xia
name: Wu, Hongzhi
name: Jin, Shoulin
Publisher: name: figshare
Year: 2024
Subjects: subject: Genetics
subject: FOS: Biological sciences

description: Supplementary Material 1
Graphosoma alkani Lodos 1959
Creators: name: Dursun, Ahmet
name: Fent, Meral
Publisher: name: Zenodo
Year: 2017
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hemiptera
Pentatomidae
Graphosoma
Graphosoma alkani

description: Graphosoma alkani Lodos, 1959
TL: Diyarbakır; 37°55'40"N, 40°14'21"E. DT: Diyarbakır (Lodos, 1959)
Note: This species is endemic to Anatolia.
description: Published as part of Dursun, Ahmet & Fent, Meral, 2017, Type Localities of Heteroptera (Insecta: Hemiptera) from Turkey, pp. 451-494 in Zootaxa 4227 (4) on page 476, DOI: 10.11646/zootaxa.4227.4.1, http://zenodo.org/record/293602
Aublysodon lateralis Cope, 1876, sp. nov.
Creators: name: Cope, E. D.
Publisher: name: Zenodo
Year: 1876
Subjects: Biodiversity
Taxonomy
Animalia
Chordata
Reptilia
Dinosauria
Tyrannosauridae
Aublysodon
Aublysodon lateralis

description: Aublysodon lateralis, sp. nov.
Established on some teeth, one of which is of the size of those of the A. horridus, and which differ in some important particulars. The posterior crenate ridge is as in that species, lateral in position, separating a posterior face from the lateral at a right angle. The posterior face is separated from that of the other side by a very obtuse angle. The anterior aspect of the crown is witlhout crenate cuttinlg edge, but the latter is present as a border to the front, passinig alonig the front of the sidle opposite to that wlhich bears the posterior angle. It is directed laterally, anld projects beyond an openi groove wlich follows its posterior base. The base of the crown is broad elliptic in section. Enamel smooth.
A much smaller tooth was found with the preceding, and presented similar characters, excepting that the posterior face is not so strongly truncate.
Measurements.M.Length of crown preserved..025Diameter of base of crownlong.018short.010Width of posteriorface.006Length of smaller crown.011Long diameter of base of do...006
The apices of both crowns are considerably worn by use. Both were found by Charles H. Sternberg of my exploring party.
description: Published as part of Cope, E. D., 1876, Descriptions of some vertebrate remains from the Fort Union beds of Montana, pp. 248-261 in Proceedings of the Academy of Natural Sciences of Philadelphia 28 on page 248, DOI: 10.5281/zenodo.3371111
Additional file 4: Table S2. of Implications of hepatitis C virus subtype 1a migration patterns for virus genetic sequencing policies in Italy
Creators: name: Cuypers, Lize
name: Vrancken, Bram
name: Fabeni, Lavinia
name: Marascio, Nadia
name: Cento, Valeria
name: Maio, Velia Di
name: Aragri, Marianna
name: Pineda-PeĂąa, Andrea
name: Schrooten, Yoeri
name: Laethem, Kristel Van
name: Balog, Daniel
name: FocĂ, Alfredo
name: Torti, Carlo
name: Nevens, Frederik
name: Perno, Carlo
name: Anne-Mieke Vandamme
name: Ceccherini-Silberstein, Francesca
Publisher: name: Figshare
Year: 2017
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Evolutionary Biology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: 60506 Virology
subject: Computational Biology

description: Geographical distribution of Italian Q80K lineages. For each Italian strain within the large Q80K clade, more detailed regional information is listed. (DOC 29Â kb)
Plagiolepis brunni Mayr, 1895, nov. spec.
Creators: name: Mayr, G.
Publisher: name: Zenodo
Year: 1895
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hymenoptera
Formicidae
Plagiolepis
Plagiolepis brunni

description: P. Brunni nov. spec.
Arbeiter. Laenge 1.4 — 1.7 Mm. Steht der P. Alluaudi Em. und der P. exigua For, sehr nahe. Glaenzend, glatt, rothgelb, Hinterleib und Beine mehr gelb, ein Fleck zwischen den Mandibelgelenken und den Augen, die Fuehlergeissel ausser dem ersten Gliede, zwei dreieckige Flecken zunaechst dem Hinterrande des ersten Abdominalsegmentes, dann eine in der Mitte unterbrochene Querbinde am zweiten Segmente, eine durchlaufende am dritten und vierten Segmente braun; auch die Basis der Mittel- und Hinterschenkel, sowie auch oft die Mittel- und Hintertibien etwas oberhalb der Mitte gebraeunt. Die Oberseite des Kopfes und des Abdomen mit einzelnen mittellangen Borstenhaaren; eine sehr feine, kurze, blassgelbe, nicht reichliche anliegende Pubescenz findet sich besonders am Kopfe und am Hinterleibe. Der Kopf ist wie bei P. Alluaudi und exigua, der Clypeus ist gekielt, der Fuehlerschaft ueberragt etwas den Hinterrand des Kopfes, die Geissei ist dicker als bei Alluaudi, nur das zweite und dritte Glied ist deutlich dicker als lang, die naechstfolgenden sind etwas laenger als dick (bei Alluaudi verhaelt sich die Geissei ebenso, bei exigua ist das zweite bis fuenfte Geisselglied dicker als lang). Das Postscutellum ist so lang als das Mesonotum oder etwas kuerzer, es ist von letzterem durch eine deutliche Furche abgeschnuert, waehrend es vom Metanotum oben durch keine Spur einer Furche, sondern nur durch die unscheinbare glatte Naht abgegrenzt ist.
Delagoabai in Suedostafrika (Dr. Brauns).
Ausser der sehr verschiedenen Faerbung und etwas verschiedener Koerperlaenge habe ich zwischen P. Alluaudi, von welchem ich ein Exemplar Herrn Prof. Emery verdanke, und P. Brunni bis jetzt fast kein sicheres Unterscheidungsmerkmal, entdecken koennen. Eine Anzahl der Arbeiter der P. Alluaudi erhielt ich von Herrn Lynch, der sie in einem Pflanzenhause in Cambridge in England gefunden hatte.
P. flavidula Rog. gehoert nach meiner Untersuchung des Typus im Berliner Museum der neungliedrigen Fuehler wegen zu Brachymyrmex und ist dem allgemeinen Ansehen nach der Prenolepis vividula aehnlich.
description: Published as part of Mayr, G., 1895, Afrikanische Formiciden., pp. 124-154 in Annalen des Naturhistorischen Museums in Wien 10 on pages 148-149
Additional file 4: Table S2. of Implications of hepatitis C virus subtype 1a migration patterns for virus genetic sequencing policies in Italy
Creators: name: Cuypers, Lize
name: Vrancken, Bram
name: Fabeni, Lavinia
name: Marascio, Nadia
name: Cento, Valeria
name: Maio, Velia Di
name: Aragri, Marianna
name: Pineda-PeĂąa, Andrea
name: Schrooten, Yoeri
name: Laethem, Kristel Van
name: Balog, Daniel
name: FocĂ, Alfredo
name: Torti, Carlo
name: Nevens, Frederik
name: Perno, Carlo
name: Anne-Mieke Vandamme
name: Ceccherini-Silberstein, Francesca
Publisher: name: Figshare
Year: 2017
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Evolutionary Biology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Virology
subject: Computational Biology

description: Geographical distribution of Italian Q80K lineages. For each Italian strain within the large Q80K clade, more detailed regional information is listed. (DOC 29Â kb)
Additional file 7: Figure S1. of Machine learning classifier for identification of damaging missense mutations exclusive to human mitochondrial DNA-encoded polypeptides
Creators: name: Martín-Navarro, Antonio
name: Gaudioso-Simón, Andrés
name: Álvarez-Jarreta, Jorge
name: Montoya, Julio
name: Mayordomo, Elvira
name: Ruiz-Pesini, Eduardo
Publisher: name: Figshare
Year: 2017
Subjects: subject: Biochemistry
subject: Genetics
subject: FOS: Biological sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: Developmental Biology

description: Representation of synthetic (SMOTE instances, red) and original (real instances, blue) dataset in the feature space. (DOC 62 kb)
QO`SHILGAN QIYMAT SOLIG`IGA OID AYRIM SHARHLAR
Creators: name: Raxmanov, Javlonbek
Publisher: name: Zenodo
Year: 2024

description: Ushbu tezis Oʻzbekistonda qoʻshilgan qiymat soligʻi hamda aylanmadan olinadigan soliq toʻlovchilarining bir-biridan farqli jihatlarini baholashga bagʻishlangan.
Breviceps acutirostris Poynton 1963
Creators: name: Conradie, Werner
name: Branch, William R.
name: Watson, Gillian
Publisher: name: Zenodo
Year: 2015
Subjects: Biodiversity
Taxonomy
Animalia
Chordata
Amphibia
Anura
Brevicipitidae
Breviceps
Breviceps acutirostris

description: Breviceps acutirostris Poynton 1963
Annals of the Natal Museum, 15(24): 321–322.
Holotype: PEM A1540 (formerly AMG 5507); Swellendam Mountains, Western Cape Province, South Africa; G. Hutchinson, 5 February 1927.
Paratypes (2): PEM A841–842 (formerly both AMG 5507); same details as holotype. Remarks. The holotype is illustrated as Fig. 31 in Poynton (1964). Both adult female holotype and paratype have a mid-right ventral incision; otherwise in perfect condition.
description: Published as part of Conradie, Werner, Branch, William R. & Watson, Gillian, 2015, Type specimens in the Port Elizabeth Museum, South Africa, including the historically important Albany Museum collection. Part 1: Amphibians in Zootaxa 3936 (1), DOI: 10.11646/zootaxa.3936.1.2, http://zenodo.org/record/237278
Prenolepis longipes Jerd
Creators: name: Mayr, G.
Publisher: name: Zenodo
Year: 1895
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hymenoptera
Formicidae
Prenolepis
Prenolepis longipes

description: P. longipes Jerd. {gracilipes Sm.).
Insel Sansibar (Dr. Brauns).
description: Published as part of Mayr, G., 1895, Afrikanische Formiciden., pp. 124-154 in Annalen des Naturhistorischen Museums in Wien 10 on page 149
Graphosoma melanoxanthum Horvath 1903
Creators: name: Dursun, Ahmet
name: Fent, Meral
Publisher: name: Zenodo
Year: 2017
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hemiptera
Pentatomidae
Graphosoma
Graphosoma melanoxanthum

description: Graphosoma melanoxanthum Horváth, 1903
TL: Azerbaijan or Armenia (valley of Arax) and Anatolia (Kasikoparan, Kulp [= Kazıkkoparan], Tuzluca, Iğdır); 40°03'33"N, 43°39'44"E.
DT: Ankara, Diyarbakır, Elazığ, Erzincan, İstanbul, Iğdır, Kars (Horváth, 1903; Ramsay, 2014; Önder et al., 2006). Note: This species have been recorded first time from Iğdır (Kazıkkoparan) with Arax Valley by Horváth (1903).
description: Published as part of Dursun, Ahmet & Fent, Meral, 2017, Type Localities of Heteroptera (Insecta: Hemiptera) from Turkey, pp. 451-494 in Zootaxa 4227 (4) on page 476, DOI: 10.11646/zootaxa.4227.4.1, http://zenodo.org/record/293602
Additional File 2:
Creators: name: Barabas, Sascha
name: Spindler, Theresa
name: Kiener, Richard
name: Tonar, Charlotte
name: Lugner, Tamara
name: Batzilla, Julia
name: Bendfeldt, Hanna
name: Rascle, Anne
name: Asbach, Benedikt
name: Wagner, Ralf
name: Deml, Ludwig
Publisher: name: Figshare
Year: 2017
Subjects: subject: Space Science
subject: Cell Biology
subject: Molecular Biology
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Positivity cut-off definition. Results obtained using the optimized IFN-γ ELISpot assay on PBMC isolated from whole blood of 45 healthy donors (Table 1 and Fig. 8) were used to generate precision profiles for each IE-1 and pp65 stimulation. Limits of quantitation (LoQ) for IE-1 and pp65 were 8.6 and 7.1 SFC/200,000 PBMC respectively. LoQ calculated on a cohort of 124 hemodialysis patients [49] were in a similar range (7.8 and 8.3 for IE-1 and pp65 respectively). Therefore, a positivity cut-off of 10 SFC/200,000 PBMC was chosen for the standardized assay. In addition, intra-assay standard deviation (SD) within both cohorts of healthy donors (n = 45) and hemodialysis patients (n = 124) for stimulated and unstimulated measurements was the basis for the calculation of a criterion that the ratio of geometric means of stimulated to unstimulated values is at least 2.5. Finally, considering a test result as positive when geometric mean for at least one of the IE-1 or pp65 stimulated approach is ≥ 10 SFC/200,000 PBMC, positive agreement (sensitivity) and negative agreement (specificity) of the optimized IFN-γ ELISpot test results with CMV serology within the collective of 45 healthy donors was 97% and 85% respectively (Fig. 8). (PDF 73 kb)
Optical conductivity of the topologically-nontrivial MXenes, Mo$_2$HfC$_2$O$_2$ and W$_2$HfC$_2$O$_2$: first-principles calculation and effective model analysis
Creators: name: Habe, Tetsuro
Publisher: name: arXiv
Year: 2025
Subjects: subject: Mesoscale and Nanoscale Physics (cond-mat.mes-hall)
subject: Materials Science (cond-mat.mtrl-sci)
subject: FOS: Physical sciences

description: The optical conductivity and the relevant electronic excitation processes are investigated in topologically-nontrivial MXenes, Mo$_2$HfC$_2$O$_2$ and W$_2$HfC$_2$O$_2$, utilizing first-principles calculation and effective model analysis. The numerical calculation based on the first-principles band structure reveals the presence of several characteristic features in the spectrum of optical conductivity as a function of photon energy. The drastic dependence on the photon polarization angle is also presented in terms of apparent features. In this paper, an effective model is also generated referring to the crystal symmetries and applied to reveal the microscopic origin of the characteristics. Then, it is shown that some features are strongly related to parity inversion between the conduction and valence bands, the key signature in electronic structures of topologically nontrivial insulators.
description: 11 pages, 7 figures
Acantholepis capensis Mayr
Creators: name: Mayr, G.
Publisher: name: Zenodo
Year: 1895
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hymenoptera
Formicidae
Acantholepis
Acantholepis capensis

description: A. capensis Mayr.
Sierra Leone (Belgisches Museum und Wiener Hofmuseum), Los-Inseln bei Senegambien an Baumstaemmen (Dr. Brauns).
description: Published as part of Mayr, G., 1895, Afrikanische Formiciden., pp. 124-154 in Annalen des Naturhistorischen Museums in Wien 10 on page 149
Graphosoma stali Horvath 1881
Creators: name: Dursun, Ahmet
name: Fent, Meral
Publisher: name: Zenodo
Year: 2017
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hemiptera
Pentatomidae
Graphosoma
Graphosoma stali

description: Graphosoma stali Horváth, 1881
Graphosoma lineatum var. s tali Horváth, 1881
TL: Akbès [=Akbez], (Hassa, Hatay); 36°51'09"N, 36°33'11"E; (Mardin); 37°18'30"N, 40°43'06"E. DT: Diyarbakır, Gaziantep, Hatay, Kars, Mardin, Muş (Horváth, 1903; Rider, 2006; Önder et al., 2006).
description: Published as part of Dursun, Ahmet & Fent, Meral, 2017, Type Localities of Heteroptera (Insecta: Hemiptera) from Turkey, pp. 451-494 in Zootaxa 4227 (4) on page 476, DOI: 10.11646/zootaxa.4227.4.1, http://zenodo.org/record/293602
Sensible Label-Free Bio-Sensing: Towards in Situ Biopsy
Creators: name: Patra, HK
Publisher: name: IEEE
Year: 2018
Subjects: Label-free
Whole cell Biosensor
Aptasensor
on/off switchable surface
early stage detection
Ellipsometry

description: © 2018 IEEE. Integrative in situ bio-sensing approaches became ominous to advance early stage detection of complex and heterogeneous disease like cancer, chronic lung, inflammation, coronary syndromes etc. Particularly in cancer, we can save million lives with existing therapeutic strategies if we can detect them at their very early stage. Unfortunately, cancer offers very rare distinctly identifiable cues during its onset to identify them and posing the technological challenges to spot them at their very early stage. We have developed an aptamer based integrated switchable rare event sensing platform using variable angle spectroscopic ellipsometry to spot in situ, readout and monitor rare events in real-time in complex heterogeneous cell population. The developed aptasensors is being tested with spotting prostate cancer cell PC3 in a blood mimicking population of human peripheral blood mononuclear cells (PBMC). The proof-of-concept data showed that the developed platform can detect in situ up to 10 target cells/ml with 10 9 background cells. The integrated sensor is designed with switchable surface to safely capture and release the target cells for further clinical validation and analysis for advanced diagnosis. We envision this alternate integrated bio-sensors strategy will be an important step towards the development of label-free in situ biopsy technique for rare event detection and early stage detection of cancer.
Additional File 2:
Creators: name: Barabas, Sascha
name: Spindler, Theresa
name: Kiener, Richard
name: Tonar, Charlotte
name: Lugner, Tamara
name: Batzilla, Julia
name: Bendfeldt, Hanna
name: Rascle, Anne
name: Asbach, Benedikt
name: Wagner, Ralf
name: Deml, Ludwig
Publisher: name: Figshare
Year: 2017
Subjects: subject: Space Science
subject: Cell Biology
subject: Molecular Biology
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Infectious Diseases
subject: FOS: Health sciences

description: Positivity cut-off definition. Results obtained using the optimized IFN-γ ELISpot assay on PBMC isolated from whole blood of 45 healthy donors (Table 1 and Fig. 8) were used to generate precision profiles for each IE-1 and pp65 stimulation. Limits of quantitation (LoQ) for IE-1 and pp65 were 8.6 and 7.1 SFC/200,000 PBMC respectively. LoQ calculated on a cohort of 124 hemodialysis patients [49] were in a similar range (7.8 and 8.3 for IE-1 and pp65 respectively). Therefore, a positivity cut-off of 10 SFC/200,000 PBMC was chosen for the standardized assay. In addition, intra-assay standard deviation (SD) within both cohorts of healthy donors (n = 45) and hemodialysis patients (n = 124) for stimulated and unstimulated measurements was the basis for the calculation of a criterion that the ratio of geometric means of stimulated to unstimulated values is at least 2.5. Finally, considering a test result as positive when geometric mean for at least one of the IE-1 or pp65 stimulated approach is ≥ 10 SFC/200,000 PBMC, positive agreement (sensitivity) and negative agreement (specificity) of the optimized IFN-γ ELISpot test results with CMV serology within the collective of 45 healthy donors was 97% and 85% respectively (Fig. 8). (PDF 73 kb)