cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
USDA Forest Service Inventory Standards and Accounting Form: Peace Keeper 92
Creators: name: Wilkes, Joan
Year: 1992
Subjects: subject: Bernalillo (County)
valueUri: https://core.tdar.org/browse/geographic-keyword/3646/bernalillo-county
subject: Albuquerque, New Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/84765/albuquerque-new-mexico
subject: Kirtland Air Force Base
valueUri: https://core.tdar.org/browse/geographic-keyword/17943/kirtland-air-force-base
subject: Historic
valueUri: https://core.tdar.org/browse/temporal-keyword/5728/historic
subject: Prehistoric
valueUri: https://core.tdar.org/browse/temporal-keyword/53/prehistoric
subject: Negative Result
valueUri: https://core.tdar.org/browse/other-keyword/76495/negative-result
subject: Cultural Resources Clearance
valueUri: https://core.tdar.org/browse/other-keyword/168090/cultural-resources-clearance
subject: Reconnaissance / Survey
valueUri: https://core.tdar.org/browse/investigation-type/7/reconnaissance-survey

description: A Cultural Resources Survey was done on acres on the Sandia Ranger District of the Cibola National Forest on the Military Withdrawal for a project involving digging fox holes for military maneuvers. The area was part of two previous surveys and nothing was found. This survey also found no cultural remains. No cultural resource will be effected. Cultural resource clearances recommended.
Supplementary document for Durable 3D models based on healthy and glaucoma-mimicking mouse retina for performance characterization of optical coherence tomography systems - 6490163.pdf
Creators: name: Barroso, Álvaro
name: Ketelhut, Steffi
name: Nettels-Hackert, Gerburg
name: Heidushka, Peter
name: del Amor, Rocío
name: Naranjo, Valery
name: Kemper, Björn
name: Schnekenburger, Juergen
Publisher: name: Optica Publishing Group
Year: 2023
Subjects: Uncategorized

description: Supplementary Figure 1
Peptide Microarrays to Interrogate the “Histone Code”
Creators: name: Krajewski, Krzysztof
name: Fuchs, Stephen M.
name: Rothbart, Scott B.
name: Strahl, Brian D.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2012

description: Histone posttranslational modifications (PTMs) play a pivotal role in regulating the dynamics and function of chromatin. Supported by an increasing body of literature, histone PTMs such as methylation and acetylation function together in the context of a “histone code,” which is read, or interpreted, by effector proteins that then drive a functional output in chromatin (e.g., gene transcription). A growing number of domains that interact with histones and/or their PTMs have been identified. While significant advances have been made in our understanding of how these domains interact with histones, a wide number of putative histone-binding motifs have yet to be characterized, and undoubtedly, novel domains will continue to be discovered. In this chapter, we provide a detailed method for the construction of combinatorially modified histone peptides, microarray fabrication using these peptides, and methods to characterize the interaction of effector proteins, antibodies, and the substrate specificity of histone-modifying enzymes. We discuss these methods in the context of other available technologies and provide a user-friendly approach to enable the exploration of histone–protein–enzyme interactions and function.
Supplementary document for DESIGN AND VALIDATION OF A CONVOLUTIONAL NEURAL NETWORK FOR FAST, MODEL-FREE BLOOD FLOW IMAGING WITH MULTIPLE EXPOSURE SPECKLE IMAGING - 6478194.pdf
Creators: name: YU, CHAO-YUEH
name: CHAMMAS, Marc
name: gurden, hirac
name: Lin, Hsin-Hon
name: Pain, Frederic
Publisher: name: Optica Publishing Group
Year: 2023
Subjects: Uncategorized

description: Supplementary_Figures_S1_S2_S3
In vitro selection of proteins with desired characteristics using mRNA-display
Creators: name: Zou, Jianwei
name: Valencia, C. Alexander
name: Liu, Rihe
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2013

description: mRNA-display is an amplification-based, iterative rounds of in vitro protein selection technique that circumvents a number of difficulties associated with yeast two-hybrid and phage display. Because of the covalent linkage between the genotype and the phenotype, mRNA-display provides a powerful means for reading and amplifying a peptide or protein sequence after it has been selected from a library with very high diversity. The purpose of this article is to provide a summary of the field and practical framework of mRNA-display-based selections. We summarize the advantages and limitations of selections using mRNA-display as well as the recent applications, namely, the identification of novel affinity reagents, target-binding partners, and enzyme substrates from synthetic peptide or natural proteome libraries. Practically, we provide a detailed procedure for performing mRNA-display-based selections with the aim of identifying protease substrates and binding partners of a target protein. Furthermore, we describe how to confirm the function of the selected protein sequences by biochemical assays and bioinformatic tools.
Supplementary document for DESIGN AND VALIDATION OF A CONVOLUTIONAL NEURAL NETWORK FOR FAST, MODEL-FREE BLOOD FLOW IMAGING WITH MULTIPLE EXPOSURE SPECKLE IMAGING - 6519140.pdf
Creators: name: YU, CHAO-YUEH
name: CHAMMAS, Marc
name: gurden, hirac
name: Lin, Hsin-Hon
name: Pain, Frederic
Publisher: name: Optica Publishing Group
Year: 2023
Subjects: Uncategorized

description: Supplementary Figures S1 S2 S3 S4
Additional file 4 of Association of Underlying Comorbidities and Sites of tuberculosis: an analysis using surveillance data
Creators: name: Jeong, Yun-Jeong
name: Kang, Ji Young
name: Kim, Hyung Woo
name: Min, Jinsoo
name: Ko, Yousang
name: Oh, Jee Youn
name: Kang, Hyeon Hui
name: Lim, Sung Chul
name: Hwang, Hun-Gyu
name: Shin, Kyeong-Cheol
name: Lee, Heung Bum
name: Kim, Ju Sang
name: Park, Jae Seuk
name: Lee, Sung Soon
name: Koo, Hyeon-Kyoung
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Biotechnology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Cancer
subject: Science Policy
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Additional file 4.
Supplementary document for Noninvasive measurement of local temperature using ultrasound-switchable fluorescence - 6538165.pdf
Creators: name: Ren, Liqin
name: Yao, Tingfeng
name: NGUYEN, KYTAI T.
name: Yuan, Baohong
Publisher: name: Optica Publishing Group
Year: 2023
Subjects: Uncategorized

description: A comparison between IR and USF images
CS Track -Expanding our knowledge on Citizen Science through analytics and analysis
Creators: name: Strähle, Michael
name: Urban, Christine
Publisher: name: Zenodo
Year: 2021
Subjects: citizen science
EU project
Horizon 2020
H2020

description: CS Track is broadening our knowledge about Citizen Science by investigating Citizen Science activities, disseminating good practices, and formulating knowledge-based policy recommendations to maximize the potential benefit of Citizen Science activities on individual citizens, organizations, and society at large and minimize potential caveats.
The Earliest Phases of Star formation (EPoS): Temperature, density, and kinematic structure of the star-forming core CB 17
Creators: name: Schmalzl, M.
name: Launhardt, R.
name: Stutz, A. M.
name: Linz, H.
name: Bourke, T. L.
name: Beuther, H.
name: Henning, Th.
name: Krause, O.
name: Nielbock, M.
name: Schmiedeke, A.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Astrophysics of Galaxies (astro-ph.GA)
subject: FOS: Physical sciences

description: Context: The initial conditions for the gravitational collapse of molecular cloud cores and the subsequent birth of stars are still not well constrained. The characteristic cold temperatures (about 10 K) in such regions require observations at sub-millimetre and longer wavelengths. The Herschel Space Observatory and complementary ground-based observations presented in this paper have the unprecedented potential to reveal the structure and kinematics of a prototypical core region at the onset of stellar birth. Aims: This paper aims to determine the density, temperature, and velocity structure of the star-forming Bok globule CB 17. This isolated region is known to host (at least) two sources at different evolutionary stages: a dense core, SMM1, and a Class I protostar, IRS. Methods: We modeled the cold dust emission maps from 100 micron to 1.2 mm with both a modified blackbody technique to determine the optical depth-weighted line-of-sight temperature and column density and a ray-tracing technique to determine the core temperature and volume density structure. Furthermore, we analysed the kinematics of CB17 using the high-density gas tracer N2H+. Results: From the ray-tracing analysis, we find a temperature in the centre of SMM1 of 10.6 K, a flat density profile with radius 9500 au, and a central volume density of n(H) = 2.3x10^5 cm-3. The velocity structure of the N2H+ observations reveal global rotation with a velocity gradient of 4.3 km/s/pc. Superposed on this rotation signature we find a more complex velocity field, which may be indicative of differential motions within the dense core. Conclusions: SMM is a core in an early evolutionary stage at the verge of being bound, but the question of whether it is a starless or a protostellar core remains unanswered.
description: published in A&A
Scientific Benchmarks for Guiding Macromolecular Energy Function Improvement
Creators: name: Tyka, Mike
name: Richardson, Jane S.
name: Davis, Ian W.
name: Jacak, Ron
name: Leaver-Fay, Andrew
name: Pache, Roland A.
name: O'Meara, Matthew J.
name: Havranek, James J.
name: Kuhlman, Brian
name: Baker, David
name: Kellogg, Elizabeth H.
name: Kortemme, Tanja
name: Gray, Jeffrey J.
name: Snoeyink, Jack
name: Lyskov, Sergey
name: Song, Yifan
name: Thompson, James
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2013

description: Accurate energy functions are critical to macromolecular modeling and design. We describe new tools for identifying inaccuracies in energy functions and guiding their improvement, and illustrate the application of these tools to improvement of the Rosetta energy function. The feature analysis tool identifies discrepancies between structures deposited in the PDB and low energy structures generated by Rosetta; these likely arise from inaccuracies in the energy function. The optE tool optimizes the weights on the different components of the energy function by maximizing the recapitulation of a wide range of experimental observations. We use the tools to examine three proposed modifications to the Rosetta energy function: improving the unfolded state energy model (reference energies), using bicubic spline interpolation to generate knowledge based torisonal potentials, and incorporating the recently developed Dunbrack 2010 rotamer library (Shapovalov and Dunbrack, 2011).
Additional file 6 of Adiposity and mortality among intensive care patients with COVID-19 and non-COVID-19 respiratory conditions: a cross-context comparison study in the UK
Creators: name: Bell, Joshua A.
name: Carslake, David
name: Hughes, Amanda
name: Tilling, Kate
name: Dodd, James W.
name: Doidge, James C.
name: Harrison, David A.
name: Rowan, Kathryn M.
name: Davey Smith, George
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Sociology
subject: FOS: Sociology
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Science Policy

description: Additional file 6: Figure S6 Adiposity, dependency and comorbidity profiles of ICU patients with COVID-19 (1 March 2020 to 31 July 2021) and non-COVID-19 respiratory conditions (1 Feb 2018 to 31 Aug 2019), by admission date.
Automated line scan analysis to quantify biosensor activity at the cell edge
Creators: name: Tsygankov, D.
name: Elston, T.C.
name: Hahn, K.M.
name: Zawistowski, J.S.
name: Allen, R.J.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2014

description: Biosensors are valuable tools used to image the subcellular localization and kinetics of protein activity in living cells. Signaling at the edge of motile cells that regulates cell protrusion and retraction is important in many aspects of cell physiology, and frequently studied using biosensors. However, quantitation and interpretation is limited by the heterogeneity of this signaling behavior; automated analytical approaches are required to systematically extract large data sets from biosensor studies for statistical analysis. Here we describe an automated analysis to relate the velocity at specific points along the cell edge with biosensor activity in adjoining regions. Time series of biosensor images are processed to interpolate a smooth edge of the cell at each time point. Profiles of biosensor activity (‘line scans’) are then calculated along lines perpendicular to the cell edge. An energy minimization method is used to calculate a velocity associated with each line scan. Sorting line scans by the proximal velocity has generated novel biological insights, as exemplified by analysis of the Src merobody biosensor. With the large data sets that can be generated automatically by this program, conclusions can be drawn that are not apparent from qualitative or ‘manual’ quantitative techniques. Our ‘LineScan’ software includes a graphical user interface (GUI) to facilitate application in other studies. It is available at hahnlab.com and is exemplified here in a study using the RhoC FLARE biosensor.
Additional file 5 of Association of Underlying Comorbidities and Sites of tuberculosis: an analysis using surveillance data
Creators: name: Jeong, Yun-Jeong
name: Kang, Ji Young
name: Kim, Hyung Woo
name: Min, Jinsoo
name: Ko, Yousang
name: Oh, Jee Youn
name: Kang, Hyeon Hui
name: Lim, Sung Chul
name: Hwang, Hun-Gyu
name: Shin, Kyeong-Cheol
name: Lee, Heung Bum
name: Kim, Ju Sang
name: Park, Jae Seuk
name: Lee, Sung Soon
name: Koo, Hyeon-Kyoung
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Biotechnology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Cancer
subject: Science Policy
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Additional file 5.
Additional file 8 of Adiposity and mortality among intensive care patients with COVID-19 and non-COVID-19 respiratory conditions: a cross-context comparison study in the UK
Creators: name: Bell, Joshua A.
name: Carslake, David
name: Hughes, Amanda
name: Tilling, Kate
name: Dodd, James W.
name: Doidge, James C.
name: Harrison, David A.
name: Rowan, Kathryn M.
name: Davey Smith, George
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Sociology
subject: FOS: Sociology
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Science Policy

description: Additional file 8: Figure S8 Respiratory support, BMI reporting and mortality profiles of ICU patients with COVID-19 (1 March 2020 to 31 July 2021) and non-COVID-19 respiratory conditions (1 Feb 2018 to 31 Aug 2019), by date of admission.
Cultural Resources Survey of 4.2 Acres for a Sandia National Laboratories Proposed Road, Kirtland Air Force Base, New Mexico
Creators: name: Hoagland, Steven R.
Publisher: name: Chambers Group, Inc.
Year: 1991
Subjects: subject: Bernalillo (County)
valueUri: https://core.tdar.org/browse/geographic-keyword/3646/bernalillo-county
subject: Albuquerque, New Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/84765/albuquerque-new-mexico
subject: Kirtland Air Force Base
valueUri: https://core.tdar.org/browse/geographic-keyword/17943/kirtland-air-force-base
subject: Historic
valueUri: https://core.tdar.org/browse/temporal-keyword/5728/historic
subject: Negative Result
valueUri: https://core.tdar.org/browse/other-keyword/76495/negative-result
subject: Cultural Resources Clearance
valueUri: https://core.tdar.org/browse/other-keyword/168090/cultural-resources-clearance
subject: Reconnaissance / Survey
valueUri: https://core.tdar.org/browse/investigation-type/7/reconnaissance-survey

description: An archaeological survey of approximately 4.2 acres of property located within Kirtland Air Force Base, Bernalillo County, New Mexico, yielded no cultural resources. It was preformed prior to construction of an access route to connect two existing facilities. The approximately 1.4 kilometers (0.87 mile) long connecting road was surveyed to a width of 12 meters (40 feet). This proposed road is situated within an area previously inventoried during the Kirtland Air Force Base, Management Area B survey (Rodgers 1980). No significant cultural resources were located within the proposed access alignment during the previous nor current archaeological surveys. Cultural resource clearance is, therefore, recommended for construction of the proposed access route as no significant cultural resources will be effected by the proposed undertaking.
From Theory to Practice to Problem: Teaching Public History with a Real Client
Creators: name: Thomas, Adam Adrian
Publisher: name: A. A. Thomas, From Theory to Practice to Problem: Teaching Public History with a Real Client, «International Public History», 2, 2019, n. 1, pp. 1-3
Year: 2019
Subjects: Public history education
Real-world projects
Clients
Scope of work

description: In many ways, teaching public history through real-world projects is a panacea. After all, a properly trained graduate of a public history program should be able to leave the theoretical world of the university and shift confidently into the exigencies of professional practice. But many educators naturally shy away from this approach because it is fraught with potential disasters – the looming threat of the unknown that frustrates students, educators, and partners alike. Flexibility and adaptability are keys to developing successful real-world public history projects. Accepting or even welcoming the unanticipated provides valuable teaching moments and a sense of reality that is difficult to duplicate in the classroom. Moreover, crafting a flexible but ultimately successful student project requires identifying the right partner or client while tempering expectations with a thoughtful and realistic scope of work.
RNA-seq in the tetraploid Xenopus laevis enables genome-wide insight in a classic developmental biology model organism
Creators: name: Osborne Nishimura, Erin
name: Conlon, Frank L.
name: Amin, Nirav M.
name: Tandon, Panna
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2014

description: Advances in sequencing technology have significantly advanced the landscape of developmental biology research. The dissection of genetic networks in model and nonmodel organisms has been greatly enhanced with high-throughput sequencing technologies. RNA-seq has revolutionized the ability to perform developmental biology research in organisms without a published genome sequence. Here, we describe a protocol for developmental biologists to perform RNA-seq on dissected tissue or whole embryos. We start with the isolation of RNA and generation of sequencing libraries. We further show how to interpret and analyze the large amount of sequencing data that is generated in RNA-seq. We explore the abilities to examine differential expression, gene duplication, transcript assembly, alternative splicing and SNP discovery. For the purposes of this article, we use Xenopus laevis as the model organism to discuss uses of RNA-seq in an organism without a fully annotated genome sequence.
Electrical Modulation of Superconducting Critical Temperature in Liquid-Gated Thin Niobium Films
Creators: name: Choi, Jiman
name: R., Pradheesh
name: Kim, Hyungsang
name: Im, Hyunsik
name: Chong, Yonuk
name: Chae, Dong-Hun
Publisher: name: arXiv
Year: 2014
Subjects: subject: Mesoscale and Nanoscale Physics (cond-mat.mes-hall)
subject: FOS: Physical sciences

description: We demonstrate that the superconducting critical temperature (Tc) of thin niobium films can be electrically modulated in a liquid-gated geometry device. Tc can be suppressed and enhanced by applying positive and negative gate voltage, respectively, in a reversible manner within a range of about 0.1 K. At a fixed temperature below Tc, we observed that the superconducting critical current can be modulated by gate voltage. This result suggests a possibility of an electrically-controlled switching device operating at or above liquid helium temperature, where superconductivity can be turned on or off solely by the applied gate voltage.
description: 7 pages, 5 figures
On time counting procedures in the DSMC method for rarefied gases
Creators: name: Rjasanow, Sergej
name: Wagner, Wolfgang
Publisher: name: Weierstrass Institute
Year: 1997
Subjects: subject: DSMC method
subject: rarefied gas dynamics
subject: Boltzmann equation
subject: time counting procedures
subject: numerical experiments
subject: 65C05
subject: 76P05
subject: 82C80

description: The DSMC (direct simulation Monte Carlo) method for rarefied gas dynamics is studied. The behaviour of the underlying stochastic particle system is determined by three main components - the time step between subsequent collisions, the random mechanism for the choice of the collision partners, and the random mechanism for calculating the result of the collision. The purpose of this paper is to illustrate the interplay between these various components and to propose some new modifications of the DSMC method. Different time counting procedures are derived and their influence on the other parts of the algorithm is investigated. Various modifications of the DSMC method are compared with respect to different criteria such as efficiency, systematic error, and statistical fluctuations.
Systematical identification of splicing regulatory cis-elements and cognate trans-factors
Creators: name: Wang, Yang
name: Wang, Zefeng
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2014

description: The majority of human genes undergo alternative splicing to generate multiple isoforms with distinct functions. This process is generally controlled by cis-acting splicing regulatory elements (SREs) that recruit trans-acting factors to promote or inhibit the use of nearby splice sites. The growing interest in understanding the regulatory rules of splicing necessitates the systematic identification of these SREs and their cognate protein factors using experimental and computational approaches. Here we describe a strategy to identify and analyze both cis-acting SREs and trans-acting splicing factors. This strategy involves a cell-based screen to identify SREs from a random sequences library and a modified RNA affinity purification approach to unbiasedly identify the splicing factors. These methods can be adopted to identify splicing enhancers or silencers in both exons and introns, and can be extended to different cultured cells. The resulting SREs and splicing factors can be further analyzed with a series of computational and experimental approaches. This approach will help us to collect a molecular part-list for splicing regulation, providing a rich data source that enables a better understanding of the “splicing code”.
Space-time random walk for stochastic differential equations in a bounded domain
Creators: name: Milstein, Grigori N.
name: Tretyakov, Michael V.
Publisher: name: Weierstrass Institute
Year: 1997
Subjects: subject: space-time Brownian motion
subject: mean-square approximation
subject: the Dirichlet problem for equations of parabolic and elliptic type
subject: exit points
subject: 60H10
subject: 60J15
subject: 65U05

description: A mean-square approximation, which ensures boundedness of both time and space increments, is considered for stochastic differential equations in a bounded domain. The proposed algorithm is based on a space-time discretization using a random walk over boundaries of small space-time parallelepipeds. To realize the algorithm, exact distributions for exit points of the space-time Brownian motion from a space-time parallelepiped are given. Convergence theorems are stated for the proposed algorithm. A method of approximate searching for exit points of the space-time diffusion from the bounded domain is constructed. Results of several numerical tests are presented.
Physical characterization and profiling of airway epithelial derived exosomes using light scattering
Creators: name: Gupta, Richa
name: Kesimer, Mehmet
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2015

description: Exosomes and other extracellular vesicles have been gaining interest during the last decade due to their emerging role in biology and, disease pathogenesis and their biomarker potential. Almost all published research related to exosomes and other extracellular vesicles include some form of physical characterization. Therefore, these vesicles should be precisely profiled and characterized physically before studying their biological role as intercellular messengers, biomarkers or therapeutic tools. Using a combination of light scattering techniques, including dynamic light scattering (DLS) and multi-angle laser light scattering combined with size exclusion separation (SEC-MALLS), we physically characterized and compared distinct extracellular vesicles derived from the apical secretions of two different cultured airway epithelial cells. The results indicated that epithelial cells release vesicles with distinct physical properties and sizes. Human primary tracheobronchial cell culture (HTBE) derived vesicles have a hydrodynamic radius (Rh) of approximately 340 nm while their radius of gyration (Rg) is approximately 200 nm. Electron microscopy analysis, however, revealed that their spherical component is 40-100 nm in size, and they carry filamentous, entangled membrane mucins on their surface that increases their overall radius. The mucin decoration on the surface defines their size and charge as measured using light scattering techniques. Their surface properties mirror the properties of the cells from which they are derived. This may provide a unique tool for researchers to elucidate the unanswered questions in normal airway biology and innate and adaptive defense, including the remodeling of airways during inflammation, tumorigenesis and metastasis.
title: A geophysical study of the Valentine Gold Project, West-Central Newfoundland
Creators: name: Abbott, Stephanie M.
Publisher: name: Memorial University of Newfoundland
Year: 2021

description: The Valentine Lake Property is located in the west-central region of the island of Newfoundland and comprises five significant structurally controlled, orogenic gold deposits. These deposits, which have proven challenging targets for geophysics, occur proximal to a major thrust faulted contact between the Precambrian Valentine Lake Intrusive Complex (VLIC), which houses the majority of gold mineralization, and the Silurian Rogerson Lake Conglomerate (RLC). Hosted within the silicic quartz-eye porphyry and trondhjemite phases of the VLIC, the gold concentrations are associated with extensional and shear parallel quartz-tourmaline-pyrite (QTP) veining. While geophysical techniques, such as induced polarization (IP), magnetics and seismic, are commonly used to detect mineral prospects, their ability to delineate the ore zone at the Valentine Gold Project (VGP) has been largely unsuccessful, primarily because the gold is scattered throughout veins within the resistive, silicic host rocks. Consequently, to date the most successful methods for locating the ore have been prospecting, soil sampling and drilling. This study employs two fresh geophysical techniques, gravity and ground-penetrating radar (GPR), supplemented by sonar surveys, in an effort to map the subsurface extent of the gold-bearing alteration zone and assess the depth of overburden and water reserves for future mine development. Throughout 2019 and 2020, a 22 line-kilometre broad-scale gravity survey, comprising 252 stations was completed, targeting the slightly less dense altered host rock. GPR data acquired over 8 priority bogs and small ponds helped define the irregular overburden to aid in gravity corrections. Combined GPR and sonar bathymetry surveys of Valentine and Victoria Lakes covering 10 and 32 square kilometres, respectively, were completed to further assist with the gravity corrections and to ascertain water resources for mining. The resulting residual Bouguer gravity map revealed a -1.7 mGal thin linear anomaly corresponding partially to the alteration zone and the bog hypsometry maps yielded overburden thicknesses up to 4.8 metres.
Counting Kinetochore Protein Numbers in Budding Yeast Using Genetically Encoded Fluorescent Proteins
Creators: name: Joglekar, Ajit P.
name: Bloom, Kerry S.
name: Salmon, E.D.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2008

description: Genetically encoded fluorescent proteins are an essential tool in cell biology, widely used for investigating cellular processes with molecular specificity. Direct uses of fluorescent proteins include studies of the in vivo cellular localization and dynamics of a protein, as well as measurement of its in vivo concentration. In this chapter, we focus on the use of genetically encoded fluorescent protein as an accurate reporter of in vivo protein numbers. Using the challenge of counting the number of copies of kinetochore proteins in budding yeast as a case study, we discuss the basic considerations in developing a technique for the accurate evaluation of intracellular fluorescence signal. This discussion includes criteria for the selection of a fluorescent protein with optimal characteristics, selection of microscope and image acquisition system components, the design of a fluorescence signal quantification technique, and possible sources of measurement errors. We also include a brief survey of available calibration standards for converting the fluorescence measurements into a number of molecules, since the availability of such a standard usually determines the design of the signal measurement technique as well as the accuracy of final measurements. Finally, we show that, as in the case of budding yeast kinetochore proteins, the in vivo intracellular protein numbers determined from fluorescence measurements can also be employed to elucidate structural details of cellular structures.
Atpūtnieki Liepājā: liepājnieki, vide un kūrorts
Creators: name: Daugavietis, Jānis
name: Āboliņa, Kristīne
name: Cērpa, Lote Katrīna
name: Garda-Rozenberga, Ieva
name: Kristberga, Laine
name: Kuduma, Anda
name: Malahovskis, Vladislavs
name: Vītola, Ieva
name: Žilde, Liāna
Publisher: name: Zenodo
Year: 2023
Subjects: Liepāja
vasaras skola
kūrorts
IDEUM
VPP
atpūtnieki

description: Prezentācija sagatavota un prezentēta vasaras skolas “Kūrortpilsētas ainavas: vēsture, kultūra un vide” ietvaros Liepājas Universitātes Kurzemes humanitārais institūtā, Kūrmājas prospektā 13. Prezentācija pieejama: https://ej.uz/KP_kopienas
In vivo Analysis of White Adipose Tissue in Zebrafish
Creators: name: Rawls, John F.
name: Minchin, James E.N.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: White adipose tissue (WAT) is the major site of energy storage in bony vertebrates, and also serves central roles in the endocrine regulation of energy balance. The cellular and molecular mechanisms underlying WAT development and physiology are not well understood. This is due in part to difficulties associated with imaging adipose tissues in mammalian model systems, especially during early life stages. The zebrafish (Danio rerio) has recently emerged as a new model system for adipose tissue research, in which WAT can be imaged in a transparent living vertebrate at all life stages. Here we present detailed methods for labeling adipocytes in live zebrafish using fluorescent lipophilic dyes, and for in vivo microscopy of zebrafish WAT.
Additional file 6 of Association of Underlying Comorbidities and Sites of tuberculosis: an analysis using surveillance data
Creators: name: Jeong, Yun-Jeong
name: Kang, Ji Young
name: Kim, Hyung Woo
name: Min, Jinsoo
name: Ko, Yousang
name: Oh, Jee Youn
name: Kang, Hyeon Hui
name: Lim, Sung Chul
name: Hwang, Hun-Gyu
name: Shin, Kyeong-Cheol
name: Lee, Heung Bum
name: Kim, Ju Sang
name: Park, Jae Seuk
name: Lee, Sung Soon
name: Koo, Hyeon-Kyoung
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Biotechnology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Cancer
subject: Science Policy
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Additional file 6.
Study of Host–Microbe Interactions in Zebrafish
Creators: name: Kim, Carol H.
name: Milligan-Myhre, Kathryn
name: Guillemin, Karen
name: Stephens, W. Zac
name: Charette, Jeremy R.
name: Rawls, John F.
name: Phennicie, Ryan T.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: All animals are ecosystems, home to diverse microbial populations.
Pseudo Three-dimensional Maps of the Diffuse Interstellar Band at 862 nm
Creators: name: Kos, Janez
name: Zwitter, Tomaž
name: Wyse, Rosemary
name: Bienaymé, Olivier
name: Binney, James
name: Bland-Hawthorn, Joss
name: Freeman, Kenneth
name: Gibson, Brad K.
name: Gilmore, Gerry
name: Grebel, Eva K.
name: Helmi, Amina
name: Kordopatis, Georges
name: Munari, Ulisse
name: Navarro, Julio
name: Parker, Quentin
name: Reid, Warren A.
name: Seabroke, George
name: Sharma, Sanjib
name: Siebert, Arnaud
name: Siviero, Alessandro
name: Steinmetz, Matthias
name: Watson, Fred G.
name: Williams, Mary E. K.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Astrophysics of Galaxies (astro-ph.GA)
subject: Instrumentation and Methods for Astrophysics (astro-ph.IM)
subject: Solar and Stellar Astrophysics (astro-ph.SR)
subject: FOS: Physical sciences

description: The diffuse interstellar bands (DIBs) are absorption lines observed in visual and near infrared spectra of stars. Understanding their origin in the interstellar medium (ISM) is one of the oldest problems in astronomical spectroscopy, as DIBs ahave been known since 1922. In a completely new approach to understanding DIBs, we combined information from nearly 500,000 stellar spectra obtained by the massive spectroscopic survey RAVE (Radial Velocity Experiment) to produce the first pseudo three-dimensional map of the strength of the DIB at 8620~Ångstroms covering the nearest 3 kiloparsecs from the Sun, and show that it follows our independently constructed spatial distribution of extinction by interstellar dust along the Galactic plane. Despite having a similar distribution in the Galactic plane, the DIB 8620 carrier has a significantly larger vertical scale height than the dust. Even if one DIB may not represent the general DIB population, our observations outline the future direction of DIB research.
description: Science 15 August 2014: 791-795, 4 pages, 3 figures
Additional file 1 of Discharge from secondary care services to primary care for adults with serious mental illness: a scoping review
Creators: name: Davis, Aubrey L. W.
name: Hamilton, Kennedy A.
name: Vozza, Jaclin A.
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Ecology
subject: FOS: Biological sciences
subject: Sociology
subject: FOS: Sociology
subject: Mental Health
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Computational Biology

description: Supplementary Material 1.
Statistical mechanics of neural networks: The Hopfield model and the Kac-Hopfield model
Creators: name: Bovier, Anton
name: Gayrard, Véronique
Publisher: name: Weierstrass Institute
Year: 1997
Subjects: subject: Hopfield model
subject: mean field theory
subject: Kac-models
subject: neural networks
subject: Gibbs measures
subject: large deviations
subject: replica symmetry
subject: 82B44
subject: 82C32
subject: 60K35

description: We survey the statistical mechanics approach to the analysis of neural networks of the Hopfield type. We consider both models on complete graphs (mean-field), random graphs (dilute model), and on regular lattices (Kac-model). We try to explain the main ideas and techniques, as well as the results obtained by them, without however going into too much technical detail. We also give a short history of the main developments in the mathematical analysis of these models over the last 20 years.
A collocation method for the solution of the first boundary value problem of elasticity in R^2
Creators: name: Elschner, Johannes
name: Hansen, Olaf
Publisher: name: Weierstrass Institute
Year: 1997
Subjects: subject: Lamé equation
subject: boundary integral equation
subject: spline collocation
subject: solvability in weighted Sobolev spaces
subject: 45E10
subject: 65N38
subject: 65R20
subject: 73C02

description: We present a spline collocation method for the numerical solution of a system of integral equations on a polygon in ĝ2. This integral equation arises if one solves the first boundary value problem for the Lamé equation with a double layer potential. The derivation and the analysis of the integral equation is given in detail. The optimal order of the spline collocation method is proved for sufficiently graded meshes.
Culture and Manipulation of Embryonic Cells
Creators: name: Edgar, Lois G.
name: Goldstein, Bob
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2012

description: The direct manipulation of embryonic cells is an important tool for addressing key questions in cell and developmental biology. C. elegans is relatively unique among genetic model systems in being amenable to manipulation of embryonic cells. Embryonic cell manipulation has allowed the identification of cell interactions by direct means, and it has been an important technique for dissecting mechanisms by which cell fates are specified, cell divisions are oriented, and morphogenesis is accomplished. Here, we present detailed methods for isolating, manipulating and culturing embryonic cells of C. elegans.
Manuel EtoPhy (Administrateurs) : Indicateurs de Risques des pesticides sur la Santé de l'Applicateur (IRSA) et de Toxicité pour l'Environnement (IRTE)
Creators: name: Mandart Elisabeth
name: Fabre Jacques
name: Philippe, Le Grusse
name: Oussama, Mghirbi
name: Ayadi Habiba
Publisher: name: Zenodo
Year: 2020
Subjects: PESTICIDE
TOXICITE DES PESTICIDES
LOGICIEL
MANUEL
AIDE A LA DECISION
EVALUATION IMPACT SUR ENVIRONNEMENT
INDICATEUR DE RISQUE POUR LA SANTE
GESTION DU RISQUE
SIMULATION
POLLUTION DIFFUSE
PLATEFORME NUMERIQUE
SERVICE
SOFTWARE
MANUAL
DIGITAL PLATFORM
RISK MANAGEMENT
HEALTH RISK INDICATOR
PESTICIDE TOXICITY
ENVIRONMENTAL IMPACT ASSESSMENT
DECISION SUPPORT

description: Ce document décrit les étapes de calcul permettant d'obtenir les Indicateurs de Risques des pesticides sur la Santé de l'Applicateur (IRSA) et de Toxicité pour l’Environnement (IRTE). Ces deux indicateurs permettent d’analyser et d’évaluer les risques liés à l’utilisation des pesticides à différentes échelles (parcelle, exploitation, région, etc.). Ils ont été conçus comme outils de diagnostic et d’aide à la décision pour optimiser la gestion des pesticides. This document describes the steps involved in calculating the Indicators of Pesticide Risk to Applicator Health (IRSA) and Environmental Toxicity (IRTE). These two indicators can be used to analyze and assess the risks associated with pesticide use at different scales (plot, farm, region, etc.). They have been designed as diagnostic and decision-making tools to optimize pesticide management.
Dobrushin-Kotecký-Shlosman theorem up to the critical temperature
Creators: name: Ioffe, Dmitry
name: Schonmann, Roberto H.
Publisher: name: Weierstrass Institute
Year: 1997
Subjects: subject: Ising model
subject: phase separation
subject: large and moderate deviations
subject: local limit theorems
subject: Wolff shape

description: We develop a non-perturbative version of the Dobrushin-Kotecký-Shlosman theory of phase separation in the canonical 2D Ising ensemble. The results are valid for all temperatures below critical.
The Optical Luminosity Function of Gamma-ray Bursts deduced from ROTSE-III Observations
Creators: name: Cui, X. H.
name: Wu, X. F.
name: Wei, J. J.
name: Yuan, F.
name: Zheng, W. K.
name: Liang, E. W.
name: Akerlof, C. W.
name: Ashley, M. C. B.
name: Flewelling, H. A.
name: Gogus, E.
name: Guver, T.
name: Kiziloglu, U.
name: McKay, T. A.
name: Pandey, S. B.
name: Rykoff, E. S.
name: Rujopakarn, W.
name: Schaefer, B. E.
name: Wheeler, J. C.
name: Yost, S. A.
Publisher: name: arXiv
Year: 2014
Subjects: subject: High Energy Astrophysical Phenomena (astro-ph.HE)
subject: FOS: Physical sciences

description: We present the optical luminosity function (LF) of gamma-ray bursts (GRBs) estimated from a uniform sample of 58 GRBs from observations with the Robotic Optical Transient Search Experiment III (ROTSE-III). Our GRB sample is divided into two sub-samples: detected afterglows (18 GRBs), and those with upper limits (40 GRBs). The $R$ band fluxes 100s after the onset of the burst for these two sub-samples are derived. The optical LFs at 100s are fitted by assuming that the co-moving GRB rate traces the star-formation rate. The detection function of ROTSE-III is taken into account during the fitting of the optical LFs by using Monte Carlo simulations. We find that the cumulative distribution of optical emission at 100s is well-described with an exponential rise and power-law decay (ERPLD), broken power-law (BPL), and Schechter LFs. A single power-law (SPL) LF, on the other hand, is ruled out with high confidence.
description: 26 pages, 3 figures, ApJ accepted
Determining absolute protein numbers by quantitative fluorescence microscopy
Creators: name: Lawrimore, Josh
name: Verdaasdonk, Jolien Suzanne
name: Bloom, Kerry
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2014

description: Biological questions are increasingly being addressed using a wide range of quantitative analytical tools to examine protein complex composition. Knowledge of the absolute number of proteins present provides insights into organization, function, and maintenance and is used in mathematical modeling of complex cellular dynamics. In this chapter, we outline and describe three microscopy-based methods for determining absolute protein numbers—fluorescence correlation spectroscopy, stepwise photobleaching, and ratiometric comparison of fluorescence intensity to known standards. In addition, we discuss the various fluorescently labeled proteins that have been used as standards for both stepwise photobleaching and ratiometric comparison analysis. A detailed procedure for determining absolute protein number by ratiometric comparison is outlined in the second half of this chapter. Counting proteins by quantitative microscopy is a relatively simple yet very powerful analytical tool that will increase our understanding of protein complex composition.
All-Optical Clock Recovery Using Multi-Section Distributed-Feedback Lasers
Creators: name: Peterhof, Daniela
name: Sandstede, Björn
Publisher: name: Weierstrass Institute
Year: 1997
Subjects: subject: Frequency entrainment
subject: aperiodic forcing
subject: laser
subject: 35B10
subject: 34C15
subject: 78A60

description: Recovering the frequency of incoming data sequences in optical transmission lines is important for signal processing. It has been suggested to use all-optical devices, for instance lasers diodes, for this purpose. Recently, self-pulsations have experimentally been discovered in multi-section distributed-feedback lasers. If a self-pulsating laser is exposed to an external data signal, it is expected that the frequency of the self-pulsation locks to the frequency of the data signal, and clock recovery would be obtained. Mathematically, this problem amounts to investigating frequency locking of periodic solutions, that is self-pulsating laser states, on invariant tori under external forcing which represents the external data sequence. In this article, Melnikov functions are derived for periodic forcing, and results on frequency locking under aperiodic forcing are given. The results are applied to a model describing the multi-section distributed-feedback lasers which have been used in experiments.
Cultural Resource Surveys Of Nine Drill Locations For Sandia National Laboratories/New Mexico, Sitewide Environmental Restoration Program, Kirtland Air Force Base, New Mexico
Creators: name: Hoagland, Steven R.
name: Dello-Russo, Robert
Publisher: name: Butler Service Group, Inc.
Year: 1995
Subjects: subject: Bernalillo (County)
valueUri: https://core.tdar.org/browse/geographic-keyword/3646/bernalillo-county
subject: Albuquerque, New Mexico
valueUri: https://core.tdar.org/browse/geographic-keyword/84765/albuquerque-new-mexico
subject: Kirtland Air Force Base
valueUri: https://core.tdar.org/browse/geographic-keyword/17943/kirtland-air-force-base
subject: Historic
valueUri: https://core.tdar.org/browse/temporal-keyword/5728/historic
subject: World War I
valueUri: https://core.tdar.org/browse/temporal-keyword/4003/world-war-i
subject: Prehistoric
valueUri: https://core.tdar.org/browse/temporal-keyword/53/prehistoric
subject: Historic
valueUri: https://core.tdar.org/browse/culture-keyword/25/historic
subject: LA 107481
valueUri: https://core.tdar.org/browse/site-name/129957/la-107481
subject: Isolated Artifact
valueUri: https://core.tdar.org/browse/site-type/344/isolated-artifact
subject: Isolated Feature
valueUri: https://core.tdar.org/browse/site-type/345/isolated-feature
subject: Homestead
valueUri: https://core.tdar.org/browse/site-type/715/homestead
subject: Chipped Stone
valueUri: https://core.tdar.org/browse/material-type/2/chipped-stone
subject: Consultation
valueUri: https://core.tdar.org/browse/investigation-type/4/consultation
subject: Reconnaissance / Survey
valueUri: https://core.tdar.org/browse/investigation-type/7/reconnaissance-survey

description: Butler Service Group Inc. (BSG) conducted cultural resource surveys for approximately 27.65 acres of property situated within Kirtland Air Force Base (KAFB), Bernalillo County, New Mexico. The areas surveyed include nine drill locales with buffer zones and an access route. One previously documented cultural resource site (LA 107481) and five isolated occurrences (IOs), were encountered during this. Site LA 107481 contains the remains of a destroyed historic homestead, that due to recordation, is no longer thought to be eligible to the National Register of Historic Places (NRHP). The five isolated occurrences are no longer thought to be potentially significant as all important information has been recovered through field recordation. Based on the apparent lack of significant cultural resources, a no effect determination is recommended for this undertaking. Document includes correspondence on the project from the Department of Energy (DOE), Department of Agriculture (DOA), and International Technology Corporation (ITC).