Additional file 1 of Loss of Ufl1/Ufbp1 in hepatocytes promotes liver pathological damage and carcinogenesis through activating mTOR signaling
Creators: name: Chen, Fanghui
name: Sheng, Le
name: Zhou, Tianci
name: Yan, Li
name: Loveless, Reid
name: Li, Honglin
name: Teng, Yong
name: Cai, Yafei
Publisher: name: figshare
Year: 2023
Subjects: subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Cancer

description: Additional file 1: Table S1. Commercial primary antibodies used in the study. Table S2. List of primers used for qRT-PCR. Table S3. Summary of the top 15 upregulated and downregulated DE proteins identified from iTRAQ assays (4-OHT vs EtOH). Supplementary Fig. S1. (A) KO efficiency determined by western blot. (B) Ratio of liver weight to body weight in 2-month-old Ufbp1Δ/Δhep mice. ** p&lt;0.01. Supplementary Fig. S2. (A) mRNA expression analysis of Collagen I after Ufl1 or Ufbp1 loss at the age of 6 months old. (B) Timeline of HFD treatment in Ufl1Δ/Δhep or Ufbp1Δ/Δhep mice, and ratios of the liver to body weight in Ufl1Δ/Δhep, Ufbp1Δ/Δhep, and their control mice after 12 weeks of HFD feeding. *** p&lt;0.001. Supplementary Fig. S3. (A) Cell viability of 4-OHT-treated and EtOH-treated groups assessed by crystal violet staining on day 6. (B) GO term enrichment for Biological Process, Cellular Component, and Molecular Function. (C) Genotypes of mouse embryo. (D) Raptor and Rictor protein levels in E11.5d embryos from Ufbp1 KO mice. ** p&lt;0.01. Supplementary Fig. S4. Ufl1 or Ufbp1 deficiency induces autophagy. (A) Raptor and Rictor protein levels in 2-month-old Ufl1Δ/Δhep, Ufbp1Δ/Δhep, and control mice. (B) LC3B and p62 proteins in 8-month-old Ufl1Δ/Δhep, Ufbp1Δ/Δhep, and control mice. (C) Immunohistochemical staining of LC3B in 8-month-old Ufbp1Δ/Δhep and control mice. Quantitative data are shown in the right panel (n=5 mice/group). (D) Immunofluorescence of LC3B in Ufbp1-/- MEFs. Quantitative data are shown in the right panel (n=5 mice/group). * p&lt;0.05; ** p&lt;0.01; *** p&lt;0.001. Supplementary Fig. S5. (A) Tumor incidence of Ufl1Δ/Δhep, Ufbp1Δ/Δhep, and their control mice at the age of 14 months old. (B) AFP gene expression in Ufbp1Δ/Δhep mouse livers at the age of 2 months and 14 months old. Quantitative data are shown in the right panel. *** p&lt;0.001. Supplementary Fig. S6. (A) Timeline of experimental procedures used to generate DEN-induced hepatocarcinogenesis. (B) Tumor incidence of Ufl1Δ/Δhep and its control mice with or without DEN induction. (C) Histopathological analysis of liver tissues extracted from 8-month-old Ufbp1Δ/Δhep and control mice with or without DEN induction. (D) Timeline of rapamycin treatment in Ufbp1Δ/Δhep and Ufl1Δ/Δhep mice. Supplementary Fig. S7. Comparison of collagen I protein levels in MEFs from E14.5d Ufbp1f/f: Rosa26Cre-ERT2 mouse embryos treated with 4-OHT vs. EtOH. Supplementary Fig. S8. Immunostaining of α-SMA in liver tissues extracted from 7-month-old Ufbp1Δ/Δhep or Ufl1Δ/Δhep mice treated with or without rapamycin. Quantitative data are shown in the right panel (n=5 mice/group). ** p&lt;0.01.