Boletus recapitulatus D. Chakr., K. Das, sp. nov.
Creators: name: Chakraborty, Dyutiparna
name: Das, Kanad
name: Baghela, Abhishek
name: Singh, Sanjay K.
name: Dentinger, Bryn T. M.
Publisher: name: Zenodo
Year: 2015
Subjects: Biodiversity
Taxonomy
Fungi
Basidiomycota
Agaricomycetes
Boletales
Boletaceae
Boletus
Boletus recapitulatus

description: Boletus recapitulatus D. Chakr., K. Das, A. Baghela, S.K. Singh & Dentinger sp. nov.   
Mycobank:—MB 811961  
GenBank:—KP938967.  
 Diagnosis:— Boletus recapitulatus is distinguished by having a low reticulum on the stipe surface, sticky reddish cap, presence of a sterile flap at pileus margin, adnate to subdecurrent tubes, angular pores with red pore mouths, yellow context quickly turning blue on exposure, narrow spores (8.0– 10.4 –12.3 × 3.2– 4.0 –5.5 μm), brown-pigmented hymenial cystidia, distinctly capitate to mushroom-shaped tips of terminal cells in pileipellis and stipitipellis, and occurrence under Betula alnoides.   
 Type:— India, Sikkim, East District, Churten, 1454m, N 27°34’10.9” E 88°25’51.9”, 25 thMay, 2014, K. Das, DC 14-001 (Holotype CAL!).   
 Etymology: recapitulatus refers to the recapitulation of the mushroom-shape among terminal cells of hyphae in the pileipellis and stipitipellis.   
 Pileus 30–90 mm diam.; convex when young, becoming plane at maturity; surface matted to subvelvety, slightly glutinous in young basidiomata, reddish brown (9D6–8), unchanging after bruising, caramel brown (6 C5–6) with KOH; margin regular to undulating with narrow sterile flap of tissue, light yellow to yellow (3 A5–6). Pore surface slightly depressed near stipe, pastel yellow to light yellow (3 A4–5) when young, gradually becoming pale orange, finally with red pore mouth on yellow background, turning greenish blue quickly after bruising; pore 2–3/mm, simple, angular. Tube 5 mm long, adnate to subdecurrent, light yellow (2 A5), quickly becoming bluish when bruised. Stipe 20–75 × 13–28 mm, central, with distinct red to brownish red (9 B – C8) low (pseudo) reticulum over vivid yellow background (3 A8) at juncture of pore surface and stipe, then just beneath the juncture brownish red to violet red (10D7– 6, 10E 5) reticulation on pastel red (8 A4) background, reticulation gradually indistinct towards base. Context mostly solid, off-white to light yellow (2 A4–5) except the stipe base which is distinctly yellow (up to 3 A6), quickly becoming bluish when exposed, turning greyish orange to brownish orange (5 B4–5 C5) with KOH, greyish yellow (3 C3–4) with FeSO 4, dull red (9 C3) with guaiacol. Odor indistinct. Taste mild. Spore print olive brown (4 E8–4 F8).   
 Basidiospores 8.0– 10.4 –12.3 × 3.2– 4.0 –5.5 μm (n = 30, Q = 1.96– 2.64 –3.23), elliptic to oblong, inequilateral, smooth under light microscope and under SEM with very high magnification (×120000) minutely warty, greenish. Basidia 17–35 ×47–9.5 μm, 4-spored, clavate to subclavate; sterigmata 5.5–16 ×1.8–9 μm. Pleurocystidia 26–60 × 4.6–10 μm, emergent 20–36 μm, common, subcylindrical, subfusiform to ventricose-rostrate or appendiculate, content dense, slightly granular, brown pigmented. Tube edge fertile, composed of basidia, cheilocystidia and chains of orange-brown pigmented palisade cells. Cheilocystidia 20–37 × 5–8 μm, common, slightly emergent (20μm), ventricose-rostrate to subcapitate to subappendiculate, contents orange-brown pigmented, granular. Hymenophoral trama divergent, tramal hyphae 6.6–8 μm wide, mostly twisted, gelatinous. Pileipellis 130–155 μm thick, trichoderm to ixotrichoderm, composed of erect septate hyphae, sometimes slightly interwoven; terminal cells 81–162 × 14–21 μm, cylindrical with rounded, subfusoid to subcapitate or distinctly capitate (or mushroom-shaped), content slightly dense. Stipitipellis 66–116 μm thick, fertile, ixotrichoderm, composed of hyphae, basidia and cystidia; caulocystidia 35–43 × 5–8 μm, subfusoid to clavate, or with rounded to subcapitate apex, contents brown; hyphae 3–5 μm wide, erect with rounded to subfusoid apex; caulobasidia similar to tube basidia but less in number. Clamp connections absent.   
 Habitat/ Distribution: Growing in groups or gregariously under Betula alnoides Buch. -Ham. ex D. Don in subtropical broadleaf forest.   
 Specimens examined: India, Sikkim, East District, Churten, 1454 m, N 27°34’10.9” E 88°25’51.9”, 25 thMay, 2014, K. Das, DC 14-001 (Holotype CAL!); ibid. East District, Churten, 1454 m, N 27°34’10.9” E 88°25’51.9”, 18 th July, 2014, D. Chakraborty, DC 14-033(CAL!).
description: Published as part of Chakraborty, Dyutiparna, Das, Kanad, Baghela, Abhishek, Singh, Sanjay K. & Dentinger, Bryn T. M., 2015, Boletus recapitulatus (Boletaceae), a new species from India with peculiar mushroom-shaped cells, pp. 150-160 in Phytotaxa 236 (2) on pages 151-152, DOI: 10.11646/phytotaxa.236.2.4, http://zenodo.org/record/13630459