title: ÐÐ¾Ð»ÑÑÐµÐ½Ð¸Ðµ ÑÐµÐºÐ¾Ð¼Ð±Ð¸Ð½Ð°Ð½ÑÐ½Ð¾Ð³Ð¾ Ð¡-ÑÐµÐ°ÐºÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° Ð² ÐºÐ»ÐµÑÐºÐ°Ñ Escherichia coli
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Production of recombinant C-reactive protein in Escherichia coli
Creators: name: ÐÐ°Ð², ÐÐ°Ð²ÐµÐ»
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð¡-ÑÐµÐ°ÐºÑÐ¸Ð²Ð½ÑÐ¹ Ð±ÐµÐ»Ð¾Ðº
ÑÐµÐºÐ¾Ð¼Ð±Ð¸Ð½Ð°Ð½ÑÐ½ÑÐ¹ Ð±ÐµÐ»Ð¾Ðº
Escherichia coli
ÑÐµÑÑ-ÑÐ¸ÑÑÐµÐ¼Ñ
ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð±ÐµÐ»ÐºÐ°
Ð¸Ð½Ð´ÑÐºÑÐ¸Ñ ÑÐ¸Ð½ÑÐµÐ·Ð° Ð±ÐµÐ»ÐºÐ°
Ð¾Ð¿ÑÐ¸Ð¼Ð¸Ð·Ð°ÑÐ¸Ñ ÑÑÐ»Ð¾Ð²Ð¸Ð¹ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð±ÐµÐ»ÐºÐ°
C-reactive protein
recombinant protein
test systems
protein expression
induction of protein synthesis
optimization of protein expression conditions

description: Ð¡-ÑÐµÐ°ÐºÑÐ¸Ð²Ð½ÑÐ¹ Ð±ÐµÐ»Ð¾Ðº (Ð¡Ð Ð) ÑÐ²Ð»ÑÐµÑÑÑ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ð¼ Ð¼Ð°ÑÐºÐµÑÐ¾Ð¼ Ð²Ð¾ÑÐ¿Ð°Ð»ÐµÐ½Ð¸Ñ. ÐÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ Ð¡Ð Ð Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÐµÑ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸ÑÐ¾Ð²Ð°ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÑ ÑÑÐ¸Ð¾Ð»Ð¾Ð³Ð¸Ñ Ð¾Ñ Ð²Ð¸ÑÑÑÐ½Ð¾Ð¹ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸, Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÑÑÑ ÑÐ¸ÑÐº ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ñ ÑÐµÑÐ´ÐµÑÐ½Ð¾-ÑÐ¾ÑÑÐ´Ð¸ÑÑÑÑ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ð¹ Ð¸ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÑÐ¾Ð²Ð°ÑÑ Ð²Ð¾ÑÐ¿Ð°Ð»Ð¸ÑÐµÐ»ÑÐ½ÑÐµ Ð¿ÑÐ¾ÑÐµÑÑÑ Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð° ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°. ÐÐ¾Ð»ÑÑÐµÐ½Ð¸Ðµ Ð¡Ð Ð Ð² Ð¿ÑÐ¾ÐºÐ°ÑÐ¸Ð¾ÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÐ¸ÑÑÐµÐ¼Ð°Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð¿Ð¾Ð·Ð²Ð¾Ð»Ð¸Ñ ÑÐ´ÐµÐ»Ð°ÑÑ Ð±Ð¾Ð»ÐµÐµ Ð±ÑÑÑÑÐ¾Ð¹ Ð¸ Ð´Ð¾ÑÑÑÐ¿Ð½Ð¾Ð¹ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÑ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÐµÑÑ-ÑÐ¸ÑÑÐµÐ¼, Ð¸ÑÐ¿Ð¾Ð»ÑÐ·ÑÑÑÐ¸Ñ Ð¡Ð Ð. ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¾Ð½Ð½Ð¾Ð³Ð¾ ÑÑÐ°Ð¼Ð¼Ð° E. coli BL21(DE3) Ñ Ð¾ÑÐ¸Ð³Ð¸Ð½Ð°Ð»ÑÐ½Ð¾Ð¹ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ð¹ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸ÐµÐ¹ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð´Ð½Ð¾Ð³Ð¾ Ð²ÐµÐºÑÐ¾ÑÐ° pQE, ÐºÐ¾Ð´Ð¸ÑÑÑÑÐµÐ¹ Ð³ÐµÐ½ crp ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°, Ð¸ Ð¾Ð¿ÑÐ¸Ð¼Ð¸Ð·Ð°ÑÐ¸Ð¸ ÑÑÐ»Ð¾Ð²Ð¸Ð¹ Ð¸Ð½Ð´ÑÐºÑÐ¸Ð¸ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð±ÐµÐ»ÐºÐ°. Ð ÑÐ°Ð¼ÐºÐ°Ñ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ» Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½ Ð´Ð¸Ð·Ð°Ð¹Ð½ Ð¾ÑÐ¸Ð³Ð¸Ð½Ð°Ð»ÑÐ½Ð¾Ð¹ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ð¹ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ð¸ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¾Ð½Ð½Ð¾Ð³Ð¾ Ð²ÐµÐºÑÐ¾ÑÐ° pQE, ÐºÐ¾Ð´Ð¸ÑÑÑÑÐµÐ¹ Ð³ÐµÐ½ crp ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°, Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Â«NestedÂ» ÐÐ¦Ð  Ð±ÑÐ»Ð° Ð¿Ð¾Ð»ÑÑÐµÐ½Ð° Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÑ Ð³ÐµÐ½Ð° crp ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°. ÐÑÐ»Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ñ ÑÑÐ°Ð¼Ð¼Ñ-Ð¿ÑÐ¾Ð´ÑÑÐµÐ½ÑÑ E. coli DH5Î± Ð¸ E. coli BL21(DE3), Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¾Ð½Ð½Ð¾Ð³Ð¾ Ð²ÐµÐºÑÐ¾ÑÐ° pQE, ÐºÐ¾Ð´Ð¸ÑÑÑÑÐµÐ³Ð¾ Ð³ÐµÐ½ crp ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°. Ð Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½Ð¾Ð¼ ÑÑÐ°Ð¼Ð¼Ðµ-Ð¿ÑÐ¾Ð´ÑÑÐµÐ½ÑÐµ E. coli BL21(DE3)_pQE-CRP Ð±ÑÐ»Ð¸ Ð¾Ð¿ÑÐ¸Ð¼Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°Ð½Ñ ÑÑÐ»Ð¾Ð²Ð¸Ñ Ð¸Ð½Ð´ÑÐºÑÐ¸Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð¡Ð Ð. ÐÐ·Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ Ð¸Ð½Ð´ÑÐºÑÐ¾ÑÐ°, Ð° ÑÐ°ÐºÐ¶Ðµ ÑÐ¼ÐµÐ½Ð° ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾ ÑÐµÐ¶Ð¸Ð¼Ð° ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ ÑÑÐ°Ð¼Ð¼Ð° Ð½Ðµ ÑÑÐ°Ð»Ð¸ ÑÐµÑÐ°ÑÑÐ¸Ð¼Ð¸ ÑÐ°ÐºÑÐ¾ÑÐ°Ð¼Ð¸ Ð¿ÑÐ¸ ÑÐ¸Ð½ÑÐµÐ·Ðµ ÑÐµÐ»ÐµÐ²Ð¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ°. ÐÐ°ÑÐ°Ð±Ð°ÑÑÐ²Ð°ÐµÐ¼ÑÐ¹ ÑÐµÐºÐ¾Ð¼Ð±Ð¸Ð½Ð°Ð½ÑÐ½ÑÐ¹ Ð±ÐµÐ»Ð¾Ðº Ð½Ð°ÑÐ¾Ð´Ð¸ÑÑÑ Ð² Ð½ÐµÑÐ°ÑÑÐ²Ð¾ÑÐ¸Ð¼Ð¾Ð¼ Ð²Ð¸Ð´Ðµ, Ð¾ ÑÑÐ¼ ÑÐ²Ð¸Ð´ÐµÑÐµÐ»ÑÑÑÐ²ÑÐµÑ Ð°Ð½Ð°Ð»Ð¸Ð· ÑÑÐ±ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ Ð»Ð¾ÐºÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸.Â 
description: C-reactive protein (CRP) is a biological marker of inflammation. Determination of CRP concentration allows differentiating bacterial etiology from viral infection, determining the risk of cardiovascular diseases and diagnosing inflammatory processes of the human body. Production of recombinant CRP in prokaryotic expression systems will make the development of diagnostic test systems utilizing CRP more rapid and affordable. This work is devoted to obtaining an expression strain of E. coli BL21(DE3) with an original genetic construct based on the plasmid vector pQE containing the human crp gene and optimizing the conditions of protein expression. In this work, the design of the original genetic construct based on the expression vector pQE encoding the human crp gene was carried out, and the sequence of the human crp gene was obtained using âNestedâ PCR. E. coli DH5Î± and E. coli BL21(DE3) strains-producers were obtained based on the expression vector pQE containing the human crp gene. In the resulting E. coli BL21(DE3)_pQE-CRP producer strain, the conditions for induction of CRP expression were optimized. Changing the inducer concentration as well as changing the temperature regime of cell strain cultivation did not become decisive factors in synthesizing the target protein. The produced recombinant protein is in insoluble form, as evidenced by the analysis of subcellular localization.