Limosilactobacillus balticus Li & Cheng & Zheng & Liu & Quevedo & Li & Roos & Gänzle & Walter 2021, SP. NOV.
Creators: name: Li, Fuyong
name: Cheng, Christopher C.
name: Zheng, Jinshui
name: Liu, Junhong
name: Quevedo, Rodrigo Margain
name: Li, Junjie
name: Roos, Stefan
name: Gänzle, Michael G.
name: Walter, Jens
Publisher: name: Zenodo
Year: 2021
Subjects: Biodiversity
Taxonomy
Bacteria
Firmicutes
Bacilli
Lactobacillales
Lactobacillaceae
Limosilactobacillus
Limosilactobacillus balticus

description: DESCRIPTION OF LIMOSILACTOBACILLUS BALTICUS SP. NOV.   
 Limosilactobacillus balticus (bal.ti′ cus. L. adj. balticus pertaining to the Baltic region where the type strain was isolated).   
 Cells are Gram-positive, non-motile, non-spore-forming, catalase-negative and heterofermentative. Cells are rod-shaped, measuring 0.9–3.0×0.6–1.0 µm. Colonies of the type strain BG-AF3-AT on MRS agar plate incubated at the anaerobic condition at 37 °C for 2 days are whitish, opaque, raised, circular and entire, with a diameter of 1.2–3.2 mm; no colony appears on MRS agar plate incubated at the aerobic condition at 37°C for 2 days. D-Lactate, L-lactate and gas are produced from glucose fermentation by the type strain BG-AF3-AT. Growth occurs at 30, 37 and 45 °C (optimum), but not at 15 ° C. Growth occurs at pH 4.0–8.0 in MRS broth. For BG-AF3-AT, the most abundant fatty acid is C 16:0, followed by C 19:0 cyclo ω 8 c. Acid is produced from L-arabinose, D-ribose,D-xylose,D-galactose,D-glucose, aesculin, maltose, lactose, melibiose, sucrose and raffinose; acid is not produced from D-fructose,D-mannose, methylα- D-glucopyranoside, potassium gluconate, glycerol, erythritol, D-arabinose, L-xylose,D-adonitol, methyl β -D-xylopyranoside,L-sorbose, L-rhamnose, dulcitol, inositol, D-mannitol,D-sorbitol, methyl α- D-mannopyranoside, N -acetylglucosamine, amygdalin, arbutin, salicin, cellobiose, trehalose, inulin, melezitose, starch, glycogen, xylitol, gentiobiose, turanose, D-lyxose, D-tagatose,D-fucose,L-fucose,D-arabitol,L-arabitol, potassium 2-ketogluconate or potassium 5-ketogluconate. The cellwall peptidoglycan of BG-AF3-AT contains the amino acids alanine (Ala), glutamic acid (Glu), lysine (Lys), and aspartic acid (Asp), with the molar ratio 1.3 (Ala):0.8 (Asp):1.0 (Glu):1.0 (Lys), suggesting the cell-wall peptidoglycan type A4α L-Lys–D-Asp by [38]. The DNA G+C content of BG-AF3-AT is 38.3mol%.   
 *API 50 CH test data of L.reuteri subsp. murium (lpuph1T and LTH5448) and L.reuteri subsp. rodentium (100-23T and TMW1.656)were obtained from [51].   
 †API 50 CH test data of L.reuteri DSM 20016T and L.vaginalis DSM 5837T were retrieved from the Bacterial Diversity Metadatabase (Bac Dive; https: //bacdive. dsmz. de/).  Fatty acid (%) L. balticus sp. nov L. agrestis sp. nov. L.. nov. L. rudii. nov. L. fastidiosus sp. nov. L. reuteri L. vaginalis  BG-AF3- AT  pH52_RY  WF-MT5- AT  BG-MG3-A Lr3000 _1_1 STM3_1 T STM2_1 WF-MO7-1 T WF-MA3-C DSM 20016 T DSM 5837 T C14:02.63.61.85.30.50.61.82.07.67.22.37.4iso-C15:00.00.00.00.00.00.00.00.00.00.20.00.0iso-C16:00.00.00.00.00.00.00.00.00.00.70.00.0C16:042.332.448.350.416.516.942.246.033.430.237.423.7C17:0 cyclo0.00.00.00.00.00.00.00.00.50.00.00.0C16:0 3-OH0.00.40.00.00.00.00.00.00.50.60.00.0 C18:1 ω 9c 6.421.75.77.331.430.16.56.617.221.26.733.3C18:03.02.55.02.53.33.35.35.71.72.27.10.0 C18:1 ω 7c 11-methyl 0.00.00.40.00.00.00.00.00.00.00.00.0C17:0 3-OH0.00.00.00.00.00.00.00.30.00.00.00.0iso-C19:01.01.30.40.02.32.51.31.20.01.00.00.0 C19:0 cyclo ω 8 c 18.98.420.413.60.00.014.215.70.00.07.00.0Summed feature 3†1.51.71.51.91.21.31.31.52.72.03.04.4Summed feature 5†0.00.00.00.00.00.00.00.00.00.017.10.0Summed feature 7†14.519.313.815.136.838.420.621.231.430.15.415.7Summed feature 8†9.98.72.64.08.17.07.00.05.24.613.915.5  albertensis sp  sp T RRLNB  
 *Cellular fatty acid data of L. reuteri DSM 20016T and L. vaginalis DSM 5837T were retrieved from the Bacterial Diversity Metadatabase (Bac Dive; https://bacdive.dsmz.de/). †Summed feature 3 comprises C16:1 ω 7 c and/or C16:1 ω 6c; summed feature 5 comprises C18:2 ω 6,9 c and/or C18:0 anteiso; summed feature 7 comprises C19:1 ω 6 c and/or C19:0 cyclo ω 10 c; summed feature 8 is an unknown combination of C18:1 ω 7 c and/or C18:1 ω 6 c.   
 The type strain is BG-AF3-AT (=DSM 110574 T =LMG 31633 T), which was isolated from the jejunum of yellow-necked mouse (Apodemus flavicollis) caught in the Vilnius area in Lithuania [13].
description: Published as part of Li, Fuyong, Cheng, Christopher C., Zheng, Jinshui, Liu, Junhong, Quevedo, Rodrigo Margain, Li, Junjie, Roos, Stefan, Gänzle, Michael G. & Walter, Jens, 2021, Limosilactobacillus balticus sp. nov., Limosilactobacillus agrestis sp. nov., Limosilactobacillus albertensis sp. nov., Limosilactobacillus rudii sp. nov. and Limosilactobacillus fastidiosus sp. nov., five novel Limosilactobacillus species isolated from the vertebrate gastrointestinal tract, and proposal of six subspecies of Limosilactobacillus reuteri adapted to the gastrointestinal tract of specific vertebrate hosts, pp. 1-21 in International Journal of Systematic and Evolutionary Microbiology (004644) (004644) 71 (2) on pages 13-16, DOI: 10.1099/ijsem.0.004644, http://zenodo.org/record/6048739