Identification of genes differentially expressed in a resistant reaction to Mycosphaerella pinodes in pea using microarray technology
Creators: name: Fondevilla, Sara
name: Küster, Helge
name: Krajinski, Franziska
name: Cubero, José I.
name: Rubiales, Diego
Publisher: name: London : BioMed Central Ltd.
Year: 2011
Subjects: subject: glutathione transferase
subject: peroxidase
subject: article
subject: Ascomycetes
subject: cell wall
subject: controlled study
subject: gene expression
subject: gene expression regulation
subject: gene identification
subject: genetic association
subject: genetic resistance
subject: genetic susceptibility
subject: membrane transport
subject: microarray analysis
subject: Mycosphaerella pinodes
subject: nonhuman
subject: nucleotide sequence
subject: protein binding
subject: protein metabolism
subject: quantitative analysis
subject: reverse transcription polymerase chain reaction
subject: signal transduction
subject: gene expression profiling
subject: genetics
subject: immunology
subject: innate immunity
subject: methodology
subject: microbiology
subject: pea
subject: physiology
subject: plant disease
subject: Ascochyta
subject: Didymella pinodes
subject: Pisum
subject: Pisum sativum
subject: Ascomycota
subject: Gene Expression Profiling
subject: Immunity, Innate
subject: Microarray Analysis
subject: Peas
subject: Plant Diseases
subject: Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie

description: Background: Ascochyta blight, caused by Mycosphaerella pinodes is one of the most important pea pathogens. However, little is known about the genes and mechanisms of resistance acting against M. pinodes in pea. Resistance identified so far to this pathogen is incomplete, polygenic and scarce in pea, being most common in Pisum relatives. The identification of the genes underlying resistance would increase our knowledge about M. pinodes-pea interaction and would facilitate the introgression of resistance into pea varieties. In the present study differentially expressed genes in the resistant P. sativum ssp. syriacum accession P665 comparing to the susceptible pea cv. Messire after inoculation with M. pinodes have been identified using a M. truncatula microarray.Results: Of the 16,470 sequences analysed, 346 were differentially regulated. Differentially regulated genes belonged to almost all functional categories and included genes involved in defense such as genes involved in cell wall reinforcement, phenylpropanoid and phytoalexins metabolism, pathogenesis- related (PR) proteins and detoxification processes. Genes associated with jasmonic acid (JA) and ethylene signal transduction pathways were induced suggesting that the response to M. pinodes in pea is regulated via JA and ET pathways. Expression levels of ten differentially regulated genes were validated in inoculated and control plants using qRT-PCR showing that the P665 accession shows constitutively an increased expression of the defense related genes as peroxidases, disease resistance response protein 39 (DRR230-b), glutathione S-transferase (GST) and 6a-hydroxymaackiain methyltransferase.Conclusions: Through this study a global view of genes expressed during resistance to M. pinodes has been obtained, giving relevant information about the mechanisms and pathways conferring resistance to this important disease. In addition, the M. truncatula microarray represents an efficient tool to identify candidate genes controlling resistance to M. pinodes in pea.