title: Ð¢ÐµÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð°Ð½ÑÐ¸Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð¿ÑÑÐ¸ ÑÐ°Ð½Ð½Ð¸Ñ Ð±ÐµÐ»ÐºÐ¾Ð² Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð° phiKZ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Testing of the antibacterial activity of five early phiKZ bacteriophage proteins
Creators: name: ÐÐµÐ¼ÐµÐ½ÐºÐ¾, ÐÐ½Ð°ÑÑÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Pseudomonas aeruginosa
Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³ phiKZ
Ð°Ð½ÑÐ¸Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½Ð°Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ
ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð³ÐµÐ½Ð¾Ð²
ÑÐ°Ð½Ð½Ð¸Ðµ Ð³ÐµÐ½Ñ
phiKZ bacteriophage
antibacterial activity
gene expression
early genes

description: Ð Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»Ð¾ÑÑ ÑÐµÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð°Ð½ÑÐ¸Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÑÐ°Ð½Ð½Ð¸Ñ Ð±ÐµÐ»ÐºÐ¾Ð² Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð° phiKZ. ÐÑÐµÐ´Ð¼ÐµÑÐ¾Ð¼ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÑÐ°Ð»Ð¾ Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ ÑÐ°Ð½Ð½Ð¸Ñ Ð³ÐµÐ½Ð¾Ð² Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð° Ð½Ð° ÑÐ¾ÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¹ Pseudomonas aeruginosa PAO1 Ð¸ Escherichia coli DH5Î±. Ð¦ÐµÐ»ÑÑ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ ÑÑÐ°Ð»Ð¾ ÐºÐ¾Ð½ÑÑÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ð¹ pHERDstr1gp275, pHERDstr1gp276, pHERDstr1gp277, pHERDstr1gp278, pHERDstr1gp279 Ð¸ Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐµÐµ Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð¿ÑÐ¾Ð´ÑÐºÑÐ¾Ð² Ð½Ð° ÑÐ¾ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Pseudomonas aeruginosa PAO1, Escherichia coli DH5É. ÐÐ»Ñ ÑÐµÑÐµÐ½Ð¸Ñ Ð¿Ð¾ÑÑÐ°Ð²Ð»ÐµÐ½Ð½Ð¾Ð¹ ÑÐµÐ»Ð¸ Ð±ÑÐ»Ð¾ Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼Ð¾ Ð²ÑÐ¿Ð¾Ð»Ð½Ð¸ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð·Ð°Ð´Ð°ÑÐ¸: Ð¿Ð¾Ð»ÑÑÐ¸ÑÑ Ð³ÐµÐ½Ñ Ð¸Ð½ÑÐµÑÐµÑÐ°; ÑÐºÐ¾Ð½ÑÑÑÑÐ¸ÑÐ¾Ð²Ð°ÑÑ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ ÑÐµÑÑÑÐ¸ÐºÑÐ°Ð·Ð½Ð¾-Ð»Ð¸Ð³Ð°Ð·Ð½Ð¾Ð³Ð¾ Ð¼ÐµÑÐ¾Ð´Ð° Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð´Ñ pHERDstr1 Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð´Ñ ÑÐ¾ Ð²ÑÑÐ°Ð²ÐºÐ°Ð¼Ð¸ ÑÐ°Ð½Ð½Ð¸Ñ Ð³ÐµÐ½Ð¾Ð² pHERDstr1gp275, pHERDstr1gp276, pHERDstr1gp277, pHERDstr1gp278, pHERDstr1gp279; Ð¿ÑÐ¾Ð²ÐµÑÑÐ¸ ÑÐ¿ÐµÐºÑÑÐ¾ÑÐ¾ÑÐ¾Ð¼ÐµÑÑÐ¸ÑÐµÑÐºÐ¸Ð¹ Ð°Ð½Ð°Ð»Ð¸Ð· ÑÐ¾ÑÑÐ° ÐºÐ»ÐµÑÐ¾Ðº Pseudomonas aeruginosa PAO1, Escherichia coli DH5É Ð¿Ð¾Ð´ Ð²Ð¾Ð·Ð´ÐµÐ¹ÑÑÐ²Ð¸ÐµÐ¼ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð¿ÑÐ¾Ð´ÑÐºÑÐ¾Ð² Ð³ÐµÐ½Ð¾Ð² gp275, gp276, gp277, gp278, gp279. Ð­ÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½Ñ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð», ÑÑÐ¾ Ð¸Ð·ÑÑÐµÐ½Ð½ÑÐµ Ð³ÐµÐ½Ñ Ð½Ðµ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÑÑÑ ÑÐ¾ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð² Ð´Ð¾ÑÑÐ°ÑÐ¾ÑÐ½Ð¾Ð¹ ÑÑÐµÐ¿ÐµÐ½Ð¸, Ð¾Ð´Ð½Ð°ÐºÐ¾ Ð³ÐµÐ½ gp277 Ð² ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ð¸ Ñ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½ÑÐ¼ Ð¾Ð±ÑÐ°Ð·ÑÐ¾Ð¼ Ð½ÐµÑÐºÐ¾Ð»ÑÐºÐ¾ Ð·Ð°Ð¼ÐµÐ´Ð»ÑÐ» ÑÐ¾ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐ¸Ð½ÐµÐ³Ð½Ð¾Ð¹Ð½Ð¾Ð¹ Ð¿Ð°Ð»Ð¾ÑÐºÐ¸. Ð¢ÐµÐ¼ Ð½Ðµ Ð¼ÐµÐ½ÐµÐµ, Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐµÐµ Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ ÑÐ°Ð½Ð½Ð¸Ñ Ð³ÐµÐ½Ð¾Ð² Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð° phiKZ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÑÐµÑ Ð·Ð½Ð°ÑÐ¸Ð¼Ð¾ÑÑÑ Ð² Ð±Ð¾ÑÑÐ±Ðµ Ñ Ð°Ð½ÑÐ¸Ð±Ð¸Ð¾ÑÐ¸ÐºÐ¾ÑÐµÐ·Ð¸ÑÑÐµÐ½ÑÐ½Ð¾ÑÑÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð¸ Pseudomonas aeruginosa Ð¸ Ð¿Ð¾Ð¸ÑÐºÐµ Ð°Ð»ÑÑÐµÑÐ½Ð°ÑÐ¸Ð²Ð½ÑÑ Ð»ÐµÐºÐ°ÑÑÑÐ²ÐµÐ½Ð½ÑÑ ÑÑÐµÐ´ÑÑÐ². ÐÑÐ¸ Ð½Ð°Ð¿Ð¸ÑÐ°Ð½Ð¸Ð¸ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±Ð·Ð¾ÑÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ: elib.spbstu.ru, NIH.gov, eLIBRARY.RU, cyberlenika.ru. Ð¡ÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÑÑ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»Ð¸ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿Ð°ÐºÐµÑÐ° Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼ Microsoft Excel.
description: In this work, the antibacterial activity of early phiKZ bacteriophage proteins was tested. The subject of the study was to study the effect of early gene expression on the growth of Pseudomonas aeruginosa PAO1 and Escherichia coli DH5a bacteria. The aim of this work was to construct genetic constructs pHERDstr1gp275, pHERDstr1gp276, pHERDstr1gp277, pHERDstr1gp278, pHERDstr1gp279 and further study the effect of genetic products on the growth ofÂ  Pseudomonas aeruginosa PAO1, Escherichia coli DH5É cells. To achieve this goal, it was necessary to perform the following tasks: to obtain the genes of interest; to construct the pHERDstr1gp275, pHERDstr1gp276, pHERDstr1gp277, pHERDstr1gp278, pHERDstr1gp279 plasmids using the restriction ligase method; to perform a spectrophotometric analysis of the growth of Pseudomonas aeruginosa PAO1, Escherichia coli DH5É cells under the influence of genetic products of gp275, gp276, gp277, gp278 genes, gp279. The experiment showed that the studied genes do not inhibit cell growth sufficiently, however, the gp277 gene, in comparison with the control sample, somewhat slowed down the growth of Pseudomonas aeruginosa cells. Nevertheless, further study of all early genes is of importance in the fight against antibiotic resistance of the bacterium Pseudomonas aeruginosa and the search for alternative medicines. When writing the literary review, the following databases were used: elib.spbstu.ru, NIH.gov, eLIBRARY.RU, cyberlenika.ru. Statistical processing of experimental data was carried out using the Microsoft Excel software package.