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title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Design a test system for the diagnosis of african swine fever virus by PCR-RT method
Creators: name: ÐÐ¾ÑÐ°ÑÐµÐ², ÐÐ¾Ð½ÑÑÐ°Ð½ÑÐ¸Ð½
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Ð²Ð¸ÑÑÑ Ð°ÑÑÐ¸ÐºÐ°Ð½ÑÐºÐ¾Ð¹ ÑÑÐ¼Ñ ÑÐ²Ð¸Ð½ÐµÐ¹ (ÐÐÐ§Ð¡)
Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð½Ð°Ñ ÑÐµÐ¿Ð½Ð°Ñ ÑÐµÐ°ÐºÑÐ¸Ñ (ÐÐ¦Ð )
Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐ°.
african swine fever virus (ASFV)
polimerase chain reaction (PCR)
diagnosis

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description: 68 pages, 10 figures, 9 tablesThe subject is Â«Design test-system for diagnosing African swine fever virus by method of PCR-RTÂ».The purpose of this scientific work is to design a diagnostic test-system based on the real-time polymerase chain reaction method for the identification of the African swine fever virus.Tasks that were solved in the course of the study:â¢ Selection of the target matrix in silico.â¢ Selection of virus isolates from the National Center for Biotechnology Information, USA (NCBI).â¢ Determination of the consensus sequence of the B646L gene and selection of primers for it.â¢ Sequencing of the B646L gene for the positive control sample (PQS), sequencing of the Cytochrome B gene for sampling control (CVM) and appropriate primers.â¢ Oligonucleotide synthesis of previously selected primers.â¢ Collecting the test system and checking its performance.Oligonucleotide synthesis, sequencing, bacterial cloning, in silico genetic sequence analyzes, real-time PCR were chosen as the main research methods.The B646L gene encoding the p72 major capsid protein of the African swine fever virus is the most conserved sequence. A diagnostic PCR-RT test-system was built on the basis of a fragment from this gene.This test-system can be used to diagnose the African swine fever virus in samples, which are buccal epithelium, blood, lymph, tissues of the affected organs.