Additional file 1 of Imprinted Grb10, encoding growth factor receptor bound protein 10, regulates fetal growth independently of the insulin-like growth factor type 1 receptor (Igf1r) and insulin receptor (Insr) genes
Creators: name: Moorwood, Kim
name: Smith, Florentia M.
name: Garfield, Alastair S.
name: Ward, Andrew
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Chemical Sciences not elsewhere classified
subject: Developmental Biology
subject: Virology

description: Additional file 1: Figures S1-S4. Fig. S1. Weights at PN1 from progeny of crosses between Grb10ins7 KO and Igf1r KO mice. Body weights are shown for the six offspring genotypes (A). Actual weights of brain (B), liver (C), lungs (D), heart (E) and kidneys (F) are shown alongside relative weights of the same organs, expressed as a percentage of body mass (G-K). Values represent means and SEM, tested using ANOVA with Kruskal-Wallis post hoc statistical tests. Sample sizes were, for body, Grb10 wild type (WT):Igf1r WT n=15, Grb10 WT:Igf1r Het n=23, Grb10 KO:Igf1r WT n=8, Grb10 KO:Igf1r Het n=18, Grb10 WT:Igf1r KO n=7, Grb10:Igf1r DKO n=12; brain, Grb10 WT:Igf1r WT n=15, Grb10 WT:Igf1r Het n=23, Grb10 KO:Igf1r WT n=8, Grb10 KO:Igf1r Het n=17, Grb10 WT:Igf1r KO n=3, Grb10:Igf1r DKO n=8 liver, Grb10 WT:Igf1r WT n=15, Grb10 WT:Igf1r Het n=23, Grb10 KO:Igf1r WT n=8, Grb10 KO:Igf1r Het n=17, Grb10 WT:Igf1r KO n=2, Grb10:Igf1r DKO n=7; lungs, Grb10 WT:Igf1r WT n=15, Grb10 WT:Igf1r Het n=23, Grb10 KO:Igf1r WT n=8, Grb10 KO:Igf1r Het n=17, Grb10 WT:Igf1r KO n=1, Grb10:Igf1r DKO n=7; heart, Grb10 WT:Igf1r WT n=14, Grb10 WT:Igf1r Het n=23, Grb10 KO:Igf1r WT n=8, Grb10 KO:Igf1r Het n=17, Grb10 WT:Igf1r KO n=2, Grb10:Igf1r DKO n=7; kidneys, Grb10 WT:Igf1r WT n=15, Grb10 WT:Igf1r Het n=23, Grb10 KO:Igf1r WT n=8, Grb10 KO:Igf1r Het n=17, Grb10 WT:Igf1r KO n=2, Grb10:Igf1r DKO n=7. Asterisks indicate p-values, * p &lt;0.05, ** p &lt;0.01, *** p &lt;0.001, **** p&lt;0.0001. Fig. S2. Weights at PN1 from progeny of crosses between Grb10Δ2-4 KO and Igf1r KO mice. Body weights are shown for the six offspring genotypes (A). Actual weights of brain (B), liver (C), lungs (D), heart (E) and kidneys (F) are shown alongside relative weights of the same organs, expressed as a percentage of body mass (G-K). Values represent means and SEM, tested using ANOVA with Kruskal-Wallis post hoc statistical tests. Sample sizes were, for body, Grb10 WT:Igf1r WT n=35, Grb10 WT:Igf1r Het n=69, Grb10 KO:Igf1r WT n=25, Grb10 KO:Igf1r Het n=67, Grb10 WT:Igf1r KO n=13, Grb10:Igf1r DKO n=28; brain, Grb10 WT:Igf1r WT n=35, Grb10 WT:Igf1r Het n=67, Grb10 KO:Igf1r WT n=25, Grb10 KO:Igf1r Het n=65, Grb10 WT:Igf1r KO n=6, Grb10:Igf1r DKO n=24 liver, Grb10 WT:Igf1r WT n=35, Grb10 WT:Igf1r Het n=69, Grb10 KO:Igf1r WT n=25, Grb10 KO:Igf1r Het n=65, Grb10 WT:Igf1r KO n=5, Grb10:Igf1r DKO n=23; lungs, Grb10 WT:Igf1r WT n=35, Grb10 WT:Igf1r Het n=69, Grb10 KO:Igf1r WT n=25, Grb10 KO:Igf1r Het n=65, Grb10 WT:Igf1r KO n=4, Grb10:Igf1r DKO n=23; heart , Grb10 WT:Igf1r WT n=34, Grb10 WT:Igf1r Het n=69, Grb10 KO:Igf1r WT n=24, Grb10 KO:Igf1r Het n=64, Grb10 WT:Igf1r KO n=5, Grb10:Igf1r DKO n=23; kidneys, Grb10 WT:Igf1r WT n=34, Grb10 WT:Igf1r Het n=66, Grb10 KO:Igf1r WT n=25, Grb10 KO:Igf1r Het n=65, Grb10 WT:Igf1r KO n=5 Grb10:Igf1r DKO n=23. Asterisks indicate p-values, * p &lt;0.05, ** p &lt;0.01, *** p &lt;0.001, **** p&lt;0.0001. Fig S3. Weights PN1 from progeny of crosses between Grb10Δ2-4 KO and Insr KO mice. Body weights are shown for the six offspring genotypes (A). Actual weights of brain (B), liver (C), lungs (D), heart (E) and kidneys (F) are shown alongside relative weights of the same organs, expressed as a percentage of body mass (G-K). Values represent means and SEM, tested using ANOVA with Kruskal-Wallis post hoc statistical tests. Sample sizes were, for body, Grb10 WT:Insr WT n=13, Grb10 WT:Insr Het n=29, Grb10 KO:Insr WT n=18, Grb10 KO:Insr Het n=26, Grb10 WT:Insr KO n=6, Grb10:Insr DKO n=9; brain, Grb10 WT:Insr WT n=13, Grb10 WT:Insr Het n=29, Grb10 KO:Insr WT n=18, Grb10 KO:Insr Het n=26, Grb10 WT:Insr KO n=6, Grb10:Insr DKO n=9; liver, Grb10 WT:Insr WT n=13, Grb10 WT:Insr Het n=29, Grb10 KO:Insr WT n=18, Grb10 KO:Insr Het n=26, Grb10 WT:Insr KO n=6, Grb10:Insr DKO n=9; lungs, Grb10 WT:Insr WT n=13, Grb10 WT:Insr Het n=29, Grb10 KO:Insr WT n=18, Grb10 KO:Insr Het n=26, Grb10 WT:Insr KO n=6, Grb10:Insr DKO n=9; heart , Grb10 WT:Insr WT n=13, Grb10 WT:Insr Het n=29, Grb10 KO:Insr WT n=18, Grb10 KO:Insr Het n=26, Grb10 WT:Insr KO n=6, Grb10:Insr DKO n=9; kidneys, Grb10 WT:Insr WT n=13, Grb10 WT:Insr Het n=29, Grb10 KO:Insr WT n=18, Grb10 KO:Insr Het n=26, Grb10 WT:Insr KO n=6, Grb10:Insr DKO n=9. Asterisks indicate p-values, * p &lt;0.05, ** p &lt;0.01, *** p &lt;0.001, **** p&lt;0.0001. Fig. S4. Litter size and weight of pups from crosses between Grb10Δ2-4 KO and Igf1r KO mice. A) Numbers of pups from different sized litters are shown according to genotype. B) Mean body weights (horizontal bars) for pups of each genotype are shown across the different litter sizes. Boxes show 25th to 75th percentiles and whiskers the range from minimum to maximum. The data are from 37 litters (mean size 4.8 pups) that contained 73 wild type, 3 Igf1r KO, 67 Grb10 KO and 14 Grb10:Igf1r DKO pups.