cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Determination of the angle of attack on a research wind turbine rotor blade using surface pressure measurements
Creators: name: Soto-Valle, Rodrigo
name: Bartholomay, Sirko
name: Alber, Jörg
name: Manolesos, Marinos
name: Nayeri, Christian Navid
name: Paschereit, Christian Oliver
Publisher: name: Technische Universität Berlin
Year: 2020
Subjects: subject: 600 Technik, Technologie
subject: research wind turbine
subject: surface pressure
subject: rotor blades

description: In this paper, a method to determine the angle of attack on a wind turbine rotor blade using a chordwise pressure distribution measurement was applied. The approach used a reduced number of pressure tap data located close to the blade leading edge. The results were compared with the measurements from three external probes mounted on the blade at different radial positions and with analytical calculations. Both experimental approaches used in this study are based on the 2-D flow assumption; the pressure tap method is an application of the thin airfoil theory, while the probe method applies geometrical and induction corrections to the measurement data. The experiments were conducted in the wind tunnel at the Hermann Föttinger Institut of the Technische Universität Berlin. The research turbine is a three-bladed upwind horizontal axis wind turbine model with a rotor diameter of 3 m. The measurements were carried out at rated conditions with a tip speed ratio of 4.35, and different yaw and pitch angles were tested in order to compare the approaches over a wide range of conditions. Results show that the pressure tap method is suitable and provides a similar angle of attack to the external probe measurements as well as the analytical calculations. This is a significant step for the experimental determination of the local angle of attack, as it eliminates the need for external probes, which affect the flow over the blade and require additional calibration.
The ribosome as an entropy trap
Creators: name: Rodnina, M. V.
name: Beringer, M.
name: Sievers, A.
name: Wolfenden, R.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2004

description: To determine the effectiveness of the ribosome as a catalyst, we compared the rate of uncatalyzed peptide bond formation, by the reaction of the ethylene glycol ester of N-formylglycine with Tris(hydroxymethyl)aminomethane, with the rate of peptidyl transfer by the ribosome. Activation parameters were also determined for both reactions, from the temperature dependence of their second-order rate constants. In contrast with most protein enzymes, the enthalpy of activation is slightly less favorable on the ribosome than in solution. The 2 × 107-fold rate enhancement produced by the ribosome is achieved entirely by lowering the entropy of activation. These results are consistent with the view that the ribosome enhances the rate of peptide bond formation mainly by positioning the substrates and/or water exclusion within the active site, rather than by conventional chemical catalysis.
A high-throughput, near-saturating screen for type III effector genes from Pseudomonas syringae
Creators: name: Chang, J. H.
name: Law, T. F.
name: Gombar, S.
name: Ausubel, F. M.
name: Arnold, L. W.
name: Urbach, J. M.
name: Dangl, J. L.
name: Grant, S. R.
name: Hu, A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2005

description: Pseudomonas syringae strains deliver variable numbers of type III effector proteins into plant cells during infection. These proteins are required for virulence, because strains incapable of delivering them are nonpathogenic. We implemented a whole-genome, high-throughput screen for identifying P. syringae type III effector genes. The screen relied on FACS and an arabinose-inducible hrpL σ factor to automate the identification and cloning of HrpL-regulated genes. We determined whether candidate genes encode type III effector proteins by creating and testing full-length protein fusions to a reporter called Δ79AvrRpt2 that, when fused to known type III effector proteins, is translocated and elicits a hypersensitive response in leaves of Arabidopsis thaliana expressing the RPS2 plant disease resistance protein. Δ79AvrRpt2 is thus a marker for type III secretion system-dependent translocation, the most critical criterion for defining type III effector proteins. We describe our screen and the collection of type III effector proteins from two pathovars of P. syringae. This stringent functional criteria defined 29 type III proteins from P. syringae pv. tomato, and 19 from P. syringae pv. phaseolicola race 6. Our data provide full functional annotation of the hrpL-dependent type III effector suites from two sequenced P. syringae pathovars and show that type III effector protein suites are highly variable in this pathogen, presumably reflecting the evolutionary selection imposed by the various host plants.
$K$-field kinks: stability, exact solutions and new features
Creators: name: Zhong, Yuan
name: Liu, Yu-Xiao
Publisher: name: arXiv
Year: 2014
Subjects: subject: High Energy Physics - Theory (hep-th)
subject: High Energy Physics - Phenomenology (hep-ph)
subject: FOS: Physical sciences

description: We study a class of noncanonical real scalar field models in $(1+1)$-dimensional flat space-time. We first derive the general criterion for the classical linear stability of an arbitrary static soliton solution of these models. Then we construct first-order formalisms for some typical models and derive the corresponding kink solutions. The linear structures of these solutions are also qualitatively analyzed and compared with the canonical kink solutions.
description: 14 pages, 3 figures
The Changing Function of Patents: A Reversion to Privileges?
Creators: name: Lai, Jessica
Publisher: name: Open Access Te Herenga Waka-Victoria University of Wellington
Year: 2023
Subjects: subject: Law
subject: FOS: Law

description: Since its inception, patent law has had many faces, manifesting different aims and functions. The latest recalibrations of the aims and functions of patent law are striking because – at its core – patent law itself has not changed significantly in this time. This paper examines the chameleon-like nature of the function of patents, tracking historical transformations from the privilege as an instrument of trade policy, to patents as an incentive/reward to invent and disclose the invention, and the most recent shift towards viewing patents as necessary for innovation. In particular, the paper addresses whether the latest shift represents a reversion to privilege-like functions, due to the analogous focus on commercialisation, and argues that this is not the case because of the fundamental move to focusing on patentees rather than society as a whole and to seeing patents as property.
Wind Turbine Tip Vortices under the influence of Wind Tunnel Blockage Effects
Creators: name: Soto-Valle, Rodrigo
name: Alber, Jörg
name: Manolesos, M.
name: Nayeri, Christian Navid
name: Paschereit, Christian Oliver
Publisher: name: Technische Universität Berlin
Year: 2020
Subjects: subject: 530 Physik
subject: wind turbines
subject: wind tunnel blockage effects
subject: wind tunnel
subject: tip vortex
subject: velocimetry measurements

description: The current paper describes the characteristics of the tip vortex in the near wake of a three-bladed upwind horizontal axis wind turbine with a rotor diameter of 3 m. Phase-locked stereo particle image velocimetry measurements were carried out under the influence of the wind tunnel walls that create a high blockage ratio. The location of the vortex, convection velocity, core radius, and strength were investigated and compared with similar investigations, including different blockages cases. Additionally, the same performance of the wind turbine model was simulated in the open source wind turbine tool QBlade, using the lifting line free vortex wake module in the absence of the walls.The results showed that the location of the tip vortices was more inboard the tip and more downstream the tunnel compared to the simulations and similar experiments. The convection velocity remained similar in the axial direction and changed in the lateral direction, contributing to the delay of the movement of the tip vortex outboard the tip. The strength, based on the circulation, was found with a difference of 4% between simulation and experiment.
Simulated Performance of Timescale Metrics for Aperiodic Light Curves
Creators: name: Findeisen, Krzysztof
name: Cody, Ann Marie
name: Hillenbrand, Lynne
Publisher: name: arXiv
Year: 2014
Subjects: subject: Instrumentation and Methods for Astrophysics (astro-ph.IM)
subject: Solar and Stellar Astrophysics (astro-ph.SR)
subject: Data Analysis, Statistics and Probability (physics.data-an)
subject: FOS: Physical sciences

description: Aperiodic variability is a characteristic feature of young stars, massive stars, and active galactic nuclei. With the recent proliferation of time domain surveys, it is increasingly essential to develop methods to quantify and analyze aperiodic variability. We develop three timescale metrics that have been little used in astronomy -- Δm-Δt plots, peak-finding, and Gaussian process regression -- and present simulations comparing their effectiveness across a range of aperiodic light curve shapes, characteristic timescales, observing cadences, and signal to noise ratios. We find that Gaussian process regression is easily confused by noise and by irregular sampling, even when the model being fit reflects the process underlying the light curve, but that Δm-Δt plots and peak-finding can coarsely characterize timescales across a broad region of parameter space. We make public the software we used for our simulations, both in the spirit of open research and to allow others to carry out analogous simulations for their own observing programs.
description: 39 pages, 16 figures, 3 tables. To be published in The Astrophysical Journal. Simulation software is available online at http://ascl.net/1408.012
The encapsulation of hydrophobic drugs in Pluronic F127 micelles: the effects of drug hydrophobicity, solution temperature and pH
Creators: name: Basak, Rajib
name: Bandyopadhyay, Ranjini
Publisher: name: arXiv
Year: 2014
Subjects: subject: Soft Condensed Matter (cond-mat.soft)
subject: FOS: Physical sciences

description: Three drugs, Ibuprofen, Aspirin and Erythromycin, are encapsulated in spherical Pluronic F127 micelles. The shapes and the size distributions of the micelles in dilute, aqueous solutions, with and without drugs, are ascertained using cryo- Scanning Electron Microscopy and Dynamic Light Scattering (DLS) experiments, respectively. Uptake of drugs above a threshold concentration is seen to reduce the critical micellization temperature of the solution. The mean hydrodynamic radii and polydispersities of the micelles are found to increase with decrease in temperature and in the presence of drug molecules. The hydration of the micellar core at lower temperatures is verified using fluorescence measurements. Increasing solution pH leads to the ionization of the drugs incorporated in the micellar cores. This causes rupture of the micelles and release of the drugs into the solution at the highest solution pH value of 11.36 investigated here and is studied using DLS and fluorescence spectrocopy.
description: 23 pages, includes 8 figures
A persistent pesticide residue and the unusual catalytic proficiency of a dehalogenating enzyme
Creators: name: Wolfenden, R. V.
name: Horvat, C. M.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2005

description: The soil of potato fields in The Netherlands harbors bacteria with the ability to metabolize 3-chloroacrylic acid, generated by the degradation of a pesticide (1,3-dichloropropene) that entered the environment in 1946. From examination of rate constants at elevated temperatures, we infer that the half-time at 25°C for spontaneous hydrolytic dechlorination of trans-3-chloroacrylic acid is 10,000 years, several orders of magnitude longer than half-times for spontaneous decomposition of other environmental pollutants such as 1,2-dichloroethane (72 years), paraoxon (13 months), atrazine (5 months), and aziridine (52 h). With thermodynamic parameters for activation similar to those for the spontaneous hydration of fumarate at pH 6.8, this slow reaction proceeds at a constant rate through the pH range between 2 and 12. However, at the active site of the enzyme 3-chloroacrylate dehalogenase (CaaD), isolated from a pseudomonad growing in these soils, hydrolytic dechlorination proceeds with a half-time of 0.18 s. Neither kcat nor kcat/Km is reduced by increasing solvent viscosity with trehalose, implying that the rate of enzymatic dechlorination is controlled by chemical events in catalysis rather than by diffusion-limited substrate binding or product release. CaaD achieves an ≈1012-fold rate enhancement, matching or surpassing the rate enhancements produced by many enzymes that act on more conventional biological substrates. One of those enzymes is 4-oxalocrotonate tautomerase, with which CaaD seems to share a common evolutionary origin.
Privacy, defamation and ZXC v Bloomberg
Creators: name: Moreham, Nicole
Publisher: name: Open Access Te Herenga Waka-Victoria University of Wellington
Year: 2023
Subjects: subject: Law
subject: FOS: Law

description: The significance of the Supreme Court decision in ZXC v Bloomberg extends well beyond its recognition of the ‘starting point’ that a person who is subject to police investigation has a reasonable expectation of privacy in respect of information about that investigation up to the point of charge. The Supreme Court’s willingness to use the privacy tort to protect a claimant’s reputational interests (based in large part on European Court of Human Rights jurisprudence) also has significant implications for the relationship between privacy and defamation. Indeed, it moves the privacy tort significantly into defamation’s domain. This explains the potential implications of that shift and shows how the measure’s like new defence foreseeable loss of reputation (recognised in ZXC itself) can help preserve the preserve essential protections for publication of reputationally damaging allegations which are true.
Absence of a desmopressin response after therapeutic expression of factor VIII in hemophilia A dogs with liver-directed neonatal gene therapy
Creators: name: Ponder, K. P.
name: Xu, L.
name: McCorquodale, S.
name: Sarkar, R.
name: Nichols, T. C.
name: Bellinger, D. A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2005

description: Hemophilia A (HA) is a bleeding disorder caused by factor VIII (FVIII) deficiency. FVIII replacement therapy can reduce bleeding but is expensive, inconvenient, and complicated by development of antibodies that inhibit FVIII activity in 30% of patients. Neonatal hepatic gene therapy could result in continuous secretion of FVIII into blood and might reduce immunological responses. Newborn HA mice and dogs that were injected i.v. with a retroviral vector (RV) expressing canine B domain-deleted FVIII (cFVIII) achieved plasma cFVIII activity that was 139 ± 22% and 116 ± 5% of values found in normal dogs, respectively, which was stable for 1.5 yr. Coagulation tests were normalized, no bleeding had occurred, and no inhibitors were detected. This is a demonstration of long-term fully therapeutic gene therapy for HA in a large animal model. Desmopressin (DDAVP; 1-deamino-[d-Arg8]vasopressin) is a drug that increases FVIII activity by inducing release of FVIII complexed with von Willebrand factor from endothelial cells. It has been unclear, however, if the FVIII is synthesized by endothelial cells or is taken up from blood. Because the plasma cFVIII in these RV-treated dogs derives primarily from transduced hepatocytes, they provided a unique opportunity to study the biology of the DDAVP response. Here we show that DDAVP did not increase plasma cFVIII levels in the RV-treated dogs, although von Willebrand factor was increased appropriately. This result suggests that the increase in FVIII in normal dogs after DDAVP is due to release of FVIII synthesized by endothelial cells.
High-Yield Production of Catalytically Active Regulatory [NiFe]-Hydrogenase From Cupriavidus necator in Escherichia coli
Creators: name: Fan, Qin
name: Caserta, Giorgio
name: Lorent, Christian
name: Zebger, Ingo
name: Neubauer, Peter
name: Lenz, Oliver
name: Gimpel, Matthias
Publisher: name: Technische Universität Berlin
Year: 2022
Subjects: subject: 570 Biowissenschaften; Biologie
subject: regulatory hydrogenase
subject: difficult-to-express protein
subject: Escherichia coli
subject: [NiFe]-hydrogenase
subject: Cupriavidus necator

description: Hydrogenases are biotechnologically relevant metalloenzymes that catalyze the reversible conversion of molecular hydrogen into protons and electrons. The O2-tolerant [NiFe]-hydrogenases from Cupriavidus necator (formerly Ralstonia eutropha) are of particular interest as they maintain catalysis even in the presence of molecular oxygen. However, to meet the demands of biotechnological applications and scientific research, a heterologous production strategy is required to overcome the low production yields in their native host. We have previously used the regulatory hydrogenase (RH) from C. necator as a model for the development of such a heterologous hydrogenase production process in E. coli. Although high protein yields were obtained, the purified enzyme was inactive due to the lack of the catalytic center, which contains an inorganic nickel-iron cofactor. In the present study, we significantly improved the production process to obtain catalytically active RH. We optimized important factors such as O2 content, metal availability, production temperature and time as well as the co-expression of RH-specific maturase genes. The RH was successfully matured during aerobic cultivation of E. coli by co-production of seven hydrogenase-specific maturases and a nickel permease, which was confirmed by activity measurements and spectroscopic investigations of the purified enzyme. The improved production conditions resulted in a high yield of about 80 mg L–1 of catalytically active RH and an up to 160-fold space-time yield in E. coli compared to that in the native host C. necator [<0.1 U (L d) –1]. Our strategy has important implications for the use of E. coli K-12 and B strains in the recombinant production of complex metalloenzymes, and provides a blueprint for the production of catalytically active [NiFe]-hydrogenases in biotechnologically relevant quantities.
Chromosomal clustering of genes controlled by the aire transcription factor
Creators: name: Benoist, C. O.
name: Venanzi, E. S.
name: Johnnidis, J. B.
name: Taxman, D. J.
name: Mathis, D. J.
name: Ting, J. P.-Y.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2005

description: Autoimmune regulator (aire) is a transcription factor that controls the self-reactivity of the T cell repertoire. Although previous results indicate that it exerts this function in part by promoting ectopic expression of a battery of peripheral-tissue antigens in epithelial cells of the thymic medulla, recent data argue for additional roles in negative selection of thymocytes by medullary cells. As one approach to exploring such roles, we performed computational analyses of microarray data on medullary RNA transcripts from aire-deficient versus wild-type mice, focusing on the genomic localization of aire-controlled genes. Our results highlight this molecule's transcriptional activating and silencing roles and reveal a significant degree of clustering of its target genes. On a local scale, aire-regulated clusters appeared punctate, with aire-controlled and aire-independent genes often being interspersed. This pattern suggests that aire's action may not be a simple reflection of the wide action of a chromatin remodeling enzyme. Analysis of the identity of certain of the clustered genes was evocative of aire's potential roles in antigen presentation and the coordination of intrathymic cell migration: for example, major histocompatibility complex class I and class II gene products and certain chemokine genes are targets of aire-regulated transcription.
Interplay of network dynamics and ties heterogeneity on spreading dynamics
Creators: name: Ferreri, Luca
name: Bajardi, Paolo
name: Giacobini, Mario
name: Perazzo, Silvia
name: Venturino, Ezio
Publisher: name: arXiv
Year: 2014
Subjects: subject: Physics and Society (physics.soc-ph)
subject: Social and Information Networks (cs.SI)
subject: Biological Physics (physics.bio-ph)
subject: Data Analysis, Statistics and Probability (physics.data-an)
subject: Populations and Evolution (q-bio.PE)
subject: FOS: Physical sciences
subject: FOS: Computer and information sciences
subject: FOS: Biological sciences

description: The structure of a network dramatically affects the spreading phenomena unfolding upon it. The contact distribution of the nodes has long been recognized as the key ingredient in influencing the outbreak events. However, limited knowledge is currently available on the role of the weight of the edges on the persistence of a pathogen. At the same time, recent works showed a strong influence of temporal network dynamics on disease spreading. In this work we provide an analytical understanding, corroborated by numerical simulations, about the conditions for infected stable state in weighted networks. In particular, we reveal the role of heterogeneity of edge weights and of the dynamic assignment of weights on the ties in the network in driving the spread of the epidemic. In this context we show that when weights are dynamically assigned to ties in the network an heterogeneous distribution is able to hamper the diffusion of the disease, contrary to what happens when weights are fixed in time.
description: 10 pages, 10 figures
Postskriptum
Creators: name: Haker, Hille
name: Ammicht Quinn, Regina
name: Junker-Kenny, Maureen
Publisher: name: Concilium
Year: 1970

description: Concilium, Bd. 40 Nr. 3 (2004): Concilium: Internationale Zeitschrift für Theologie, Band 40, Heft 3
Confidentiality and spatially explicit data: Concerns and challenges
Creators: name: VanWey, L. K.
name: Rindfuss, R. R.
name: Gutmann, M. P.
name: Entwisle, B.
name: Balk, D. L.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2005

description: Recent theoretical, methodological, and technological advances in the spatial sciences create an opportunity for social scientists to address questions about the reciprocal relationship between context (spatial organization, environment, etc.) and individual behavior. This emerging research community has yet to adequately address the new threats to the confidentiality of respondent data in spatially explicit social survey or census data files, however. This paper presents four sometimes conflicting principles for the conduct of ethical and high-quality science using such data: protection of confidentiality, the social–spatial linkage, data sharing, and data preservation. The conflict among these four principles is particularly evident in the display of spatially explicit data through maps combined with the sharing of tabular data files. This paper reviews these two research activities and shows how current practices favor one of the principles over the others and do not satisfactorily resolve the conflict among them. Maps are indispensable for the display of results but also reveal information on the location of respondents and sampling clusters that can then be used in combination with shared data files to identify respondents. The current practice of sharing modified or incomplete data sets or using data enclaves is not ideal for either the advancement of science or the protection of confidentiality. Further basic research and open debate are needed to advance both understanding of and solutions to this dilemma.
Diagnostic and phylogenetic significance of lateral field incisures, phasmids and other morphological characters of second-stage juveniles and males of Heteroderidae (Nematoda, Tylenchida), with notes on hosts and phylogeography
Creators: name: Sturhan, Dieter
Publisher: name: Russian Journal of Nematology
Year: 2018
Subjects: Ataloderinae
Cryphodera
distribution
Globodera
Heterodera
Heteroderinae
Hylonema
Meloidodera
Meloidoderinae
morphology
plant hosts
Punctoderinae
Sarisodera
Verutinae
Verutus.

description: Data of the current knowledge about the number of incisures in the lateral fields of secondstage juveniles and males, shape of phasmids and tip of spicules of the 158 recognised species in the 19 genera in the family Heteroderidae are reviewed and compiled. All characters are of diagnostic value in distinguishing genera and intrageneric species groups, but more detailed studies are needed. Differences in the number of incisures or in the size of phasmids among species of Cryphodera, Meloidodera and Atalodera are considered as an indication of paraphyly. In agreement with results of molecular analyses, three additional species groups are proposed for Heterodera species with Poales as hosts (Cardiolata, Koreana and Orientalis groups), and in Globodera designation of Rostochiensis, Millefolii and Zelandica groups. Information on host ranges and host reaction to parasitism has great relevance for the study of phylogenetic relationships and estimation of the age of taxa. Evaluation of biogeographical data restricted to ‘natural’ occurrence as well as consideration of geological and palaeoclimatic events are essential for the recovery of putative ancestral areas of origin of taxa. Heteroderid populations tentatively identified as Meloidodera, Hylonema and Sarisodera are briefly characterised from Africa (the first two genera) and Vietnam (the third genus). Other populations of unidentified species or representatives of still unknown genera are reported from Borneo, the Canary Islands, the Dominican Republic, New Zealand, Thailand, Tunisia and Vietnam.
Morphological variation and molecular study of the root lesion nematode Pratylenchus thornei
Creators: name: Nafiseh Divsalar
name: Shokoohi, Ebrahim
name: Hoseinipour, Akbar
name: Fourie, Hendrika
name: Esmat Mahdikhani Moqaddam
Publisher: name: Russian Journal of Nematology
Year: 2018
Subjects: Iran
morphometric
phylogeny
28S rDNA

description: During a survey of plant-parasitic nematodes in the northern part of Iran, six populations of Pratylenchus thornei were studied by means of morphological and molecular characterisation. The results showed that body length has a highly significant correlation with all morphometric data indexed in the measurement table, except for the c’ index and the dorsal pharyngeal gland opening. However, the highest correlation was detected between body length and ‘a’ index (r = 0.805). Furthermore, stylet length had a significant correlation with body length (r = 0.511), tail length (r = 0.300) and V (r = 0.324). On the study of ratios, the results revealed that b and a are more reliable to evaluate the species. A molecular study of the P. thornei populations using 28S rDNA showed close relationships of the Iranian and USA, Morocco, Moldova, Spain and United Kingdom populations extracted from NCBI Genbank. The results indicated monophyly of P. thornei. Measurement table, illustrations and phylogenetic analysis for this species are given.
Exploiting the orthogonal constraints offered by high-precision age and chemistry
Creators: name: Casali, Giada
Publisher: name: Zenodo
Year: 2023

description: In the era of large spectroscopic surveys, high-quality spectra can contribute to our understanding of the Galactic chemical evolution, not only providing high-precision abundances of elements belonging to the different nucleosynthesis channels, but also giving constraints to one of the most elusive quantities in astrophysics, i.e. stellar age. In this talk, I will briefly show how high-quality spectra (HARPS@TNG and FIES@NOT) of a sample of Kepler stars, of which we know high-precision ages from asteroseismology, can enhance our ability to calibrate stellar age-chemistry relationships. The orthogonal constraints offered by age from asteroseismology, precise chemistry from ground-based high-resolution spectroscopy, and dynamics from the Gaia mission can open the door to a more accurate understanding of the Galactic chemical evolution, tracing the processes of chemical enrichment with time.
Correction of factor IX deficiency in mice by embryonic stem cells differentiated in vitro
Creators: name: Stafford, D. W.
name: Pleasant, W. A.
name: Hatada, S.
name: Fair, J. H.
name: Smithies, O.
name: Kim, H.-s.
name: LaPaglia, M. A.
name: Cairns, B. A.
name: Meyer, A. A.
name: Frelinger, J. A.
name: Gui, T.
name: Caballero, M.
name: Pevny, L.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2005

description: Murine embryonic stem (ES) cells are pluripotent, but significant functional engraftment does not occur when they are introduced into the liver. However, here we demonstrate that functional liver engraftment does occur if the ES cells (from strain 129 mice) are first differentiated in vitro for 7 days in the presence of FGF. Strikingly, when these differentiated cells, termed putative endodermal precursors (PEPs), were injected into their livers, two of six C57BL/6 and four of eight BALB/c factor IX (F-IX)-deficient mice survived for >7 days, even though the recipients were of a different strain and, in the case of the BALB/c recipients, had a complete MHC mismatch. F-IX was detected in all six of the PEP-injected survivors. Two mice subsequently died of causes unrelated to F-IX; the others survived until death at 38 or 115 days after the transplantation. No uninjected control F-IX-deficient mice survived for >7 days. Large confluent regions of sinusoidal PEP engraftment were demonstrated by immunofluorescence in the long-term BALB/c survivors. The PEP engraftment was not associated with detectable cell fusion, and the transplantation was accompanied with only a low incidence of teratoma formation.
Making Qualitative Data Reusable
Creators: name: Braukmann, Ricarda
Publisher: name: Zenodo
Year: 2023

description: Slides of the presentation given at the event "I want to be FAIR - How to deal with my qualitative data?" In this presentation, we present our guidebook for making qualitative reusable. The guidebook summarises the challenges and provides guidance on how reusability of qualitative data can be improved at all stages of the research data life cycle. It also includes a decision tree outlining different options for reuse.
Constraining Inert Triplet Dark Matter by the LHC and FermiLAT
Creators: name: Ayazi, Seyed Yaser
name: Firouzabadi, S. Mahdi
Publisher: name: arXiv
Year: 2014
Subjects: subject: High Energy Physics - Phenomenology (hep-ph)
subject: FOS: Physical sciences

description: We study collider phenomenology of inert triplet scalar dark matter at the LHC. We discuss possible decay of Higgs boson to dark matter candidate and apply current experimental data for invisible Higgs decay and $R_{γγ}$ to constrain parameter space of our model. We also investigate constraints on dark matter coming from forthcoming measurement, $R_{Zγ}$ and mono-Higgs production. We analytically calculate the annihilation cross section of dark matter candidate into $2γ$ and $Zγ$ and then use FermiLAT data to put constraints on parameter space of Inert Triplet Model. We found that this limit can be stronger than the constraints provided by LUX experiment for low mass DM.
description: 22 pages, 7 figures, Accepted in JCAP
Klerikaler Kindesmissbrauch und das Zusammenwirken von staatlichem und kirchlichem Recht
Creators: name: Torfs, Rik
Publisher: name: Concilium
Year: 1970

description: Concilium, Bd. 40 Nr. 3 (2004): Concilium: Internationale Zeitschrift für Theologie, Band 40, Heft 3
Report of Heterodera mothi Khan & Husain, 1965 (Tylenchida, Heteroderidae) in Azerbaijan
Creators: name: Ryss, Alexander Yu.
name: Kazimova, Gulnara
name: Subbotin, Sergei A.
Publisher: name: Russian Journal of Nematology
Year: 2018
Subjects: Aras River Valley
Caucasus
molecular phylogeny
taxonomy
wheat field

description: The description of the Heterodera mothi from wheat field of Azerbaijan, Aras River Valley, is given. Morphology of female cysts and the second-stage juveniles, and the phylogenetic relationships of H. mothi within the genus Heterodera based on the ITS rRNA and COI mtDNA genes, show that species belongs to the Cyperi group. This is a first record of the H. mothi in Azerbaijan and Caucasus.
Nurses' reported use of standing orders in primary health care settings
Creators: name: Wilkinson, Jillian
Publisher: name: Open Access Te Herenga Waka-Victoria University of Wellington
Year: 2023
Subjects: subject: 111799 Public Health and Health Services not elsewhere classified
subject: FOS: Health sciences
subject: 111099 Nursing not elsewhere classified

description: INTRODUCTION: The Medicines (Standing Order) Amendment Regulations 2011 allow medicines to be supplied or administered to a patient by a nurse in the absence of a medical practitioner and without a prescription. Regulations have been in place since 2002, but no substantive research has occurred in New Zealand concerning their use. AIM: This paper reports a survey of registered nurses (RNs) who work in primary health care (PHC) settings and explores aspects of their practice relating to their use of standing orders. METHODS: A self-reported survey using a non-probability sample of RNs working in PHC who use standing orders in their practice (n=231). Data were analysed descriptively. RESULTS: The sample were experienced RNs (mean 24 years since registration) and 53% have a postgraduate qualification. Some nurses' understanding of a standing order included provision of a prescription to a patient. Standing orders were used frequently (42% reported use 1 to >5 times/day) for a wide variety of conditions. There is a significant relationship between undertaking the stated professional development requirements and confidence in the clinical decisions made (p=0.025). Over half (52%) would like to use standing orders more often. DISCUSSION: Standing orders are used extensively in PHC settings. The conditions nurses are involved in treating are usually already differentiated or have a high degree of diagnostic certainty. Nurses can effectively provide medicines under standing orders when doctors support their use, issue evidence-based orders, and have confidence in nurses with advanced skills. Doctors need to meet their responsibilities under the Regulations.
De novo cis-regulatory module elicitation for eukaryotic genomes
Creators: name: Gupta, M.
name: Liu, J. S.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2005

description: Transcription regulation is controlled by coordinated binding of one or more transcription factors in the promoter regions of genes. In many species, especially higher eukaryotes, transcription factor binding sites tend to occur as homotypic or heterotypic clusters, also known as cis-regulatory modules. The number of sites and distances between the sites, however, vary greatly in a module. We propose a statistical model to describe the underlying cluster structure as well as individual motif conservation and develop a Monte Carlo motif screening strategy for predicting novel regulatory modules in upstream sequences of coregulated genes. We demonstrate the power of the method with examples ranging from bacterial to insect and human genomes.
Heat stability of resistance in selected tomato breeding lines against Meloidogyne incognita and M. javanica populations under elevated soil temperatures
Creators: name: Awol Seid
name: Chemeda Fininsa
name: Tesfamariam M. Mekete
name: Wesemael, Wim M. L.
name: Decraemer, Wilfrida
Publisher: name: Russian Journal of Nematology
Year: 2018
Subjects: climate change
exposure time
management
Mi-1
Meloidogyne species

description: In tomato, the only commercially available source of resistance to root-knot nematodes (RKN) is the Mi-1 gene that confers resistance to Meloidogyne incognita, M. javanica and M. arenaria. However, its effectiveness was limited at higher soil temperatures. A study was initiated with the objective to check the durability of the potential resistance genes found in some tomato breeding lines after screening in controlled glasshouse conditions ≤ 27°C by exposing them to higher soil temperatures at 28, 32 and 36°C for 24 and 48 h periods. The aggressive Jittu and Babile M. incognita and Jittu and Koka M. javanica populations originally collected from Ethiopia were used. When seedlings reached the four-leaf stage, each tube was inoculated with 50 freshly (≤ 24 h) hatched infective second-stage juveniles (J2). Immediately after inoculation, the seedlings were exposed continuously for 24 and 48 h in a warm water bath at 28, 32 and 36°C, respectively. A control was kept separately in ambient temperature (24°C ± 2°C). The external ambient temperature and the soil temperature inside the tube while in the water bath were simultaneously recorded using a TESTO data logger. Temperature, tomato breeding lines and time had a significant effect on the number of J2 of Jittu and Babile M. incognita and Jittu and Koka M. javanica populations that penetrated the roots. The utility of the potential resistance found in the breeding lines during the controlled growth chamber resistance screening experiment was limited at higher soil temperatures, especially at 32 and 36°C. At 36°C there was no significant difference found on the mean number of penetrated J2 of Jittu and Babile M. incognita and Jittu and Koka M. javanica populations inside the roots of all the tested breeding lines compared to ‘Marmande’ (a susceptible control) after 48 h of heat exposure after inoculation. More J2 were found in the roots of the tested breeding lines after 48 h compared to 24 h heat exposure after inoculation for each soil temperature level tested and for both populations of M. incognita. It is clear from our observations that local tomato breeding lines with resistance potential can be used when soil temperatures remain below 32°C. Differences were observed between breeding lines depending on the RKN population used at higher temperatures and this knowledge can help in further optimising the development of sustainable resistance under local Ethiopian circumstances.
Lernen trotz fehlender Worte. Erkennen, Verstehen und Fördern von Kindern mit einer umschriebenen Sprachentwicklungsstörung im Unterricht
Creators: name: Beerli, Manuela
Publisher: name: Zenodo
Year: 2022
Subjects: Sonderpädagogik
Masterarbeit
Umschriebene Sprachentwicklungsstörung
Kinder

description: Kinder mit einer umschriebenen Sprachentwicklungsstörung zeigen meist ein hochkomplexes Störungsbild. Ohne die passende Diagnose und Kenntnisse über die Auswirkungen einer umschriebenen Sprachentwicklungsstörung auf das Lernen ist eine adäquate Förderung und die Bildungszukunft dieser Kinder stark gefährdet. Mit dieser Arbeit wird erforscht, wie eine umschriebene Sprachentwicklungsstörung im Unterricht erkannt werden kann und wie sich diese auf das schulische Lernen auswirkt. Im Fokus dieser Arbeit steht die Fragestellung, inwiefern sich die in der Praxis beobachtete Häufung von fachspezifischem Förderbedarf der Kinder mit einer umschriebenen Sprachentwicklungsstörung in der Primarschule über ihr primäres Störungsbild erklären lässt. Untersucht werden die zwei Fachbereiche Mathematik- und Fremdsprachenlernen. Abgeleitet aus den Erkenntnissen werden pädagogisch-didaktische Handlungsempfehlungen für den Unterricht formuliert. Die Beantwortung der Fragestellung stützt sich auf aktuelle Fachartikel und wissenschaftliche Studien über die Entwicklung und das Lernen von Kindern mit einer umschriebenen Sprachentwicklungsstörung allgemein sowie spezifisch über deren Lernen im Mathematik- und Fremdsprachenunterricht. Berücksichtigt wird weiter Literatur zum sprachheilpädagogischen Unterricht, Fachtexte zur allgemeinen Förderung bei Lernschwierigkeiten im Mathematik- und Fremdsprachenlernen sowie Praxiserfahrungen aus dem Unterricht an der Sprachheilschule. Die Ergebnisse zeigen, dass sich eine umschriebene Sprachentwicklungsstörung sehr vielfältig auf das schulische Lernen auswirken kann. In Bezug auf die Fachbereiche Mathematik- und Fremdsprachenlernen haben Kinder mit einer umschriebenen Sprachentwicklungsstörung ein höheres Risiko, Lernschwierigkeiten zu entwickeln, als sprachunauffällige Kinder. Die fachspezifischen Lernschwierigkeiten lassen sich direkt oder indirekt auf das primäre Störungsbild, die umschriebene Sprachentwicklungsstörung, beziehungsweise auf gemeinsame Drittfaktoren zurückführen. Die formulierten pädagogisch-didaktischen Handlungsempfehlungen lassen sich nicht rezepthaft auf den Unterricht übertragen, sondern sollen entsprechend der individuellen Ausprägung des Störungsbildes ausgewählt und angepasst werden. Das erarbeitete Wissen wurde in drei Broschüren zusammengefasst und in zwei Gruppendiskussionen mit Expertinnen evaluiert.
Direct observation of thymine dimer repair in DNA by photolyase
Creators: name: Zhong, D.
name: Saxena, C.
name: Wang, L.
name: Sancar, A.
name: Kao, Y.-T.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2005

description: Photolyase uses light energy to split UV-induced cyclobutane dimers in damaged DNA, but its molecular mechanism has never been directly revealed. Here, we report the direct mapping of catalytic processes through femtosecond synchronization of the enzymatic dynamics with the repair function. We observed direct electron transfer from the excited flavin cofactor to the dimer in 170 ps and back electron transfer from the repaired thymines in 560 ps. Both reactions are strongly modulated by active-site solvation to achieve maximum repair efficiency. These results show that the photocycle of DNA repair by photolyase is through a radical mechanism and completed on subnanosecond time scale at the dynamic active site, with no net change in the redox state of the flavin cofactor.
Adaptive texture energy measure method
Creators: name: Ertugrul, Omer Faruk
Publisher: name: arXiv
Year: 2014
Subjects: subject: Computer Vision and Pattern Recognition (cs.CV)
subject: FOS: Computer and information sciences

description: Recent developments in image quality, data storage, and computational capacity have heightened the need for texture analysis in image process. To date various methods have been developed and introduced for assessing textures in images. One of the most popular texture analysis methods is the Texture Energy Measure (TEM) and it has been used for detecting edges, levels, waves, spots and ripples by employing predefined TEM masks to images. Despite several success- ful studies, TEM has a number of serious weaknesses in use. The major drawback is; the masks are predefined therefore they cannot be adapted to image. A new method, Adaptive Texture Energy Measure Method (aTEM), was offered to over- come this disadvantage of TEM by using adaptive masks by adjusting the contrast, sharpening and orientation angle of the mask. To assess the applicability of aTEM, it is compared with TEM. The accuracy of the classification of butterfly, flower seed and Brodatz datasets are 0.08, 0.3292 and 0.3343, respectively by TEM and 0.0053, 0.2417 and 0.3153, respectively by aTEM. The results of this study indicate that aTEM is a successful method for texture analysis.
Comparative analysis of excretory-secretory proteins of nematodes Trichinella spiralis and Trichinella pseudospiralis muscle larvae
Creators: name: Odoevskaya, Irina M.
name: Kudryashova, Irina B.
name: Kurnosova, Olga P.
name: Rekstina, Valentina V.
name: Rudenskaya, Yulia A.
name: Ziganshin, Rustam H.
name: Kalebina, Tatyana S.
Publisher: name: Russian Journal of Nematology
Year: 2018
Subjects: Trichinellosis
functional proteins
LC-MS/MS analysis

description: Analysis of excretory-secretory (E-S) proteins produced by muscle larvae of Trichinella spiralis strain MH 259593 and Trichinella pseudospiralis strain KU 357408 by liquid chromatographytandem mass spectrometry revealed the presence of important functional proteins in both strains: deoxyribonuclease family proteins, 5’-nucleotidases and serine proteases. The revealed proteins showed different degree of identity with proteins previously found in E-S products of Trichinella, for which immunoreactivity was shown (Bien et al., 2015), as well as with those predicted based on the results of DNA and RNA sequence analysis. The results of this work point to the importance of further investigation of the variety of E-S proteins, obtained from Trichinella domestic strains of different taxonomic origin, to find early diagnostic antigens or potential candidates for development of a polyvalent vaccine.
Electrically monitoring DNA repair by photolyase
Creators: name: Sancar, A.
name: Barton, J. K.
name: DeRosa, M. C.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2005

description: Cyclobutane pyrimidine dimers are the major DNA photoproducts produced upon exposure to UV radiation. If left unrepaired, these lesions can lead to replication errors, mutation, and cell death. Photolyase is a light-activated flavoenzyme that binds to pyrimidine dimers in DNA and repairs them in a reaction triggered by electron transfer from the photoexcited flavin cofactor to the dimer. Using gold electrodes modified with DNA duplexes containing a cyclobutane thymine dimer (T<>T), here we probe the electrochemistry of the flavin cofactor in Escherichia coli photolyase. Cyclic and square-wave voltammograms of photolyase deposited on these electrodes show a redox signal at 40 mV versus normal hydrogen electrode, consistent with electron transfer to and from the flavin in the DNA-bound protein. This signal is dramatically attenuated on surfaces where the π-stacking of the DNA bases is perturbed by the presence of an abasic site below the T<>T, an indication that the redox pathway is DNA-mediated. DNA repair can, moreover, be monitored electrically. Exposure of photolyase on T<>T-damaged DNA films to near-UV/blue light leads to changes in the flavin signal consistent with repair, as confirmed by parallel HPLC experiments. These results demonstrate the exquisite sensitivity of DNA electrochemistry to perturbations in base pair stacking and the applicability of this chemistry to probe reactions of proteins with DNA.
Factors that influence new graduates’ preferences for specialty areas
Creators: name: Wilkinson, Jillian
name: Neville, Stephen
name: Huntington, Annette
name: Watson, Paul
Publisher: name: Open Access Te Herenga Waka-Victoria University of Wellington
Year: 2023
Subjects: subject: 111099 Nursing not elsewhere classified
subject: FOS: Health sciences

description: No description supplied
Cyclic electrical activity in the intestine of marine nematode Enoplus brevis
Creators: name: Slivko-Koltchik, Georgy A.
name: Kuznetsov, Victor P.
name: Voronov, Dmitry A.
name: Panchin, Yuri V.
Publisher: name: Russian Journal of Nematology
Year: 2018
Subjects: gap junctions
intercellular communication
membrane potential
central pattern generator
nematode defecation

description: In the nematode Caenorhabditis elegans the defecation motor program (DMP) is initiated by calcium concentration oscillations between intestinal cells without participation of the nervous system. In the related nematode Heterorhabditis megidis it was shown that DMP is accompanied by electrical membrane potential oscillations in the intestinal cells. Calcium oscillations and membrane potential in these species are cell-autonomous, and they are synchronised between cells through gap junctions. In the midgut cells of distant from Rhabditida nematode Enoplus brevis (Enoplida) we discovered membrane potential cycling which resemble oscillations in H. megidis. We also found that the gut cells in E. brevis are electrically connected through gap junctions. This finding indicates that distant nematode species possess the same non-nervous rhythmical generation DMP mechanism. It appears that this mechanism was present in the nematode last common ancestor.