Investigation of biophysical mechanisms in gold nanoparticle mediated laser manipulation of cells using a multimodal holographic and fluorescence imaging setup
Creators: name: Kalies, Stefan
name: Antonopoulos, Georgios C.
name: Rakoski, Mirko S.
name: Heinemann, Dag
name: Schomaker, Markus
name: Ripken, Tammo
name: Meyer, Heiko
Publisher: name: San Francisco, CA : Public Library of Science (PLoS)
Year: 2015
Subjects: subject: F actin
subject: gold nanoparticle
subject: actin
subject: gold
subject: metal nanoparticle
subject: animal cell
subject: Article
subject: biophysics
subject: calcium cell level
subject: calcium signaling
subject: cell function
subject: cell manipulation
subject: cell viability
subject: cell volume
subject: controlled study
subject: cytoplasm
subject: cytoskeleton
subject: dog
subject: fluorescence imaging
subject: genetic transfection
subject: holography
subject: ion exchange
subject: laser
subject: multimodal imaging
subject: nonhuman
subject: phototherapy
subject: radiation exposure
subject: cell line
subject: cell survival
subject: chemistry
subject: metabolism
subject: procedures
subject: Actins
subject: Calcium Signaling
subject: Cell Line
subject: Cell Survival
subject: Gold
subject: Holography
subject: Lasers
subject: Metal Nanoparticles
subject: Multimodal Imaging
subject: Optical Imaging
subject: Dewey Decimal Classification::500 | Naturwissenschaften
subject: Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit

description: Laser based cell manipulation has proven to be a versatile tool in biomedical applications. In this context, combining weakly focused laser pulses and nanostructures, e.g. gold nanoparticles, promises to be useful for high throughput cell manipulation, such as transfection and photothermal therapy. Interactions between laser pulses and gold nanoparticles are well understood. However, it is still necessary to study cell behavior in gold nanoparticle mediated laser manipulation. While parameters like cell viability or perforation efficiency are commonly addressed, the influence of the manipulation process on other essential cell parameters is not sufficiently investigated yet. Thus, we set out to study four relevant cell properties: cell volume and area, ion exchange and cytoskeleton structure after gold nanoparticle based laser manipulation. For this, we designed a multimodal imaging and manipulation setup. 200 nm gold nanoparticles were attached unspecifically to canine cells and irradiated by weakly focused 850 ps laser pulses. Volume and area change in the first minute post laser manipulation was monitored using digital holography. Calcium imaging and cells expressing a marker for filamentous actin (F-actin) served to analyze the ion exchange and the cytoskeleton, respectively. High radiant exposures led to cells exhibiting a tendency to shrink in volume and area, possibly due to outflow of cytoplasm. An intracellular raise in calcium was observed and accompanied by an intercellular calcium wave. This multimodal approach enabled for the first time a comprehensive analysis of the cell behavior in gold nanoparticle mediated cell manipulation. Additionally, this work can pave the way for a better understanding and the evaluation of new applications in the context of cell transfection or photothermal therapy.