Additional file 2 of Inflammation of the choroid plexus in progressive multiple sclerosis: accumulation of granulocytes and T cells
Creators: name: Sabela Rodríguez-Lorenzo
name: Konings, Julia
name: Pol, Susanne
name: Kamermans, Alwin
name: Amor, Sandra
name: Horssen, Jack
name: Witte, Maarten
name: Kooij, Gijs
name: Vries, Helga
Publisher: name: figshare
Year: 2020
Subjects: subject: Biophysics
subject: Biochemistry
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Neuroscience
subject: Physiology
subject: Immunology
subject: FOS: Clinical medicine
subject: FOS: Clinical medicine
subject: Developmental Biology
subject: Cancer
subject: Hematology
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: FOS: Health sciences
subject: 60506 Virology

description: Additional file 2: Figure S1. Immune cells located in the vessel compartment of the CP of progressive MS patients and controls. a) Iba1+ cells and their MHCII expression. b) Iba1- MHCII+ cells. c) CD3+ T cells. d) CD19+ B cells. e) CD138+ plasma cells. f) CD66b + granulocytes (Wilcoxon rank sum test with continuity correction). C: control; PMS: progressive MS. Figure S2. T cells in close contact with APCs in the CP stroma. a) Representative images of the CP immunolabeled with CD3 (green) and MHCII (red); vessels are visualized with UEA I (white). White arrowheads point to a CD3+ T cell in close contact with an MHCII+ APC (left panel), and to a non-interacting T cell (right panel). b) On the top panel, three T cells can be seen: one is not interacting with any MHCII+ cell (white arrow), while the other two are in close contact with MHCII+ cells (white arrowheads). The middle and lower panels show higher magnification of T lymphocytes interacting with APCs. c) Percentage of CD4+ T cells and CD8+ T cells interacting with APCs in the CP of control and progressive MS patients, defined as the T cells located directly adjacent to MHCII+ cells (Wilcoxon rank sum test with continuity correction). Scale bar is 10 μm. Figure S3. Most granulocytes in the CP are neutrophils. Representative images of one CP section immunolabeled with CD66b (red) and elastase (green). Maximum projection image. White arrowheads point to CD66b + elastase+ neutrophils. Scale bars are 50 μm. Figure S4. PCA plot of the samples used in this study, showing standardized principal components 1 and 2. Axes show the percentage of variance explained by each principal component. Variables included in the analysis: density of CP MHCII+ macrophages, MHCII- macrophages, DCs, total T cells, CD4+ and CD8+ T cells, percentage of T cells interacting with MHCII+ cells, B or plasma cells and granulocytes. PC: principal component; PMS: progressive MS. Figure S5. PPMS and SPMS patients present similar non-circulating (stromal and epithelium-associated) immune cell subsets in the CP. a) Density of non-circulating CD3+ T cells in PPMS and SPMS patients (Welch Two Sample t-test). b) Ratio of non-circulating CD4+ vs CD8+ T cells in PPMS and SPMS patients (Welch Two Sample t-test). c) Density of non-circulating MHCII+ macrophages in PPMS and SPMS patients (Welch Two Sample t-test). d) Density of non-circulating MHCII- macrophages in PPMS and SPMS patients (Welch Two Sample t-test). d) Density of non-circulating Iba1-MHCII+ DCs in PPMS and SPMS patients (Wilcoxon rank sum test). e) Density of non-circulating granulocytes in PPMS and SPMS patients (Wilcoxon rank sum test). PPMS: Primary Progressive MS; SPMS: Secondary Progressive MS