Novomicrothelia pandanicola S. N. Zhang & K. D. Hyde 2017, sp. nov.
Creators: name: Zhang, Sheng-Nan
name: Hyde, Kevin D.
name: Jones, E. B. Gareth
name: Cheewangkoon, Ratchadawan
name: Boonmee, Saranyaphat
name: Doilom, Mingkwan
name: Mapook, Ausana
name: Liu, Jian-Kui
Publisher: name: Zenodo
Year: 2017
Subjects: Biodiversity
Taxonomy
Fungi
Ascomycota
Dothideomycetes
Trypetheliales
Trypetheliaceae
Novomicrothelia
Novomicrothelia pandanicola

description: Novomicrothelia pandanicola S.N. Zhang & K.D. Hyde, sp. nov.
Index Fungorum number: IF 553176; Facesoffungi number: FoF 03289; Figure 2.
Family: Trypetheliaceae
Etymology: Name reflects to the host Pandanus tectorius, from which the holotype was collected.
Holotype: MFLU 17-0753
Saprobic on the bark of Pandanus tectorius, with a tropical distribution. Sexual morph: Thallus ecorticate, non-lichenized, some part bordered by dark prothallus lines. Ascomata 490–990 μm diam., 190–200 μm high, solitary or irregularly confluent, immersed to erumpent, subglobose to flask-shaped, covered with black pseudoclypeus, in vertical section 183–305 μm diam., 177–248 μm high. Ostioles apical or rarely eccentric. Peridium 18–29 μm wide, with 5–6 layers of compressed cells of textura angularis, coriaceous, partly carbonaceous, dimidiate, comprising a mixture of host cells and brown fungal hyphae, K+ (olivaceous within 10% KOH solution). Hamathecium up to 1.5 μm wide, hyaline, filamentous, sparsely septate, branched and anastomosing pseudoparaphyses, embedded in a gelatinous matrix. Asci 75–137 × 12–20 μm, (x = 93.8 × 14.5 μm, n = 18), 8-spored, bitunicate, narrowly clavate to subcylindrical, pedicellate, occasionally constricted at the upper part, apically rounded, with a small ocular chamber, up to 1.5 μm high. Ascospores 16–32 × 5–10 μm (x = 24 × 7.3 μm, n = 30), hyaline, becoming brown with age, partly overlapping or biseriate, ellipsoid to obovoid or fusiform, verruculose, muriform and elongated, with 3–7 transverse septa, and 1–4 longitudinal septa in all cells and rarely in end cells, sometimes strongly constricted at the middle septum, straight or slightly curved, wall smooth, surrounded by an irregular, gelatinous sheath, observed more clearly when mounted in Indian ink. Asexual morph: Undetermined.
Culture characteristics:—Ascospores germinating on PDA within 48 hours at room temperature in natural light. Germ tubes produced from each end. Colonies growing well on both PDA and MEA media, and attaining a diameter of 1.5 cm on PDA after two months at room temperature, slow growing, obverse olive to grey-green or light grey-green, tufted colony center elevated, reverse dark green (Figure 3). Mycelium 2–3 μm wide, hyaline to pale brown, aerial, septate, ornamented, branched and anastomosing. Chlamydospores 7.7–43 × 5−11.5 (x = 16.8 × 7.3 μm, n = 20), produced from mycelia, initially subglobose with 1 cell, hyaline, becoming 1–5 septate, pale brown to dark brown.
Material examined:— THAILAND, Chanthaburi, Ao Khung Kraben, on dead bark of Pandanus tectorius (Pandanaceae R.Br.), 7 July 2016, M. Doilom SN-T-04 (MFLU 17-0753, holotype); ex-type culture MFLUCC 16- 1382 = GZCC 17-0004. (HKAS 96378, isotype).
description: Published as part of Zhang, Sheng-Nan, Hyde, Kevin D., Jones, E. B. Gareth, Cheewangkoon, Ratchadawan, Boonmee, Saranyaphat, Doilom, Mingkwan, Mapook, Ausana & Liu, Jian-Kui, 2017, Novomicrothelia pandanicola sp. nov., a non-lichenized Trypetheliaceae species from Pandanus, pp. 254-264 in Phytotaxa 321 (3) on pages 258-260, DOI: 10.11646/phytotaxa.321.3.3, http://zenodo.org/record/13697696