Timaviella dunensis Mikhailyuk & O. M. Vynogr. 2022, sp. nov.
Creators: name: Mikhailyuk, Tatiana
name: Vinogradova, Oksana
name: Holzinger, Andreas
name: Glaser, Karin
name: Akimov, Yuri
name: Karsten, Ulf
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Bacteria
Cyanobacteria
Cyanophyceae
Synechococcales
Oculatellaceae
Timaviella
Timaviella dunensis

description: Timaviella dunensis Mikhailyuk & O.M. Vynogr. sp. nov. (Figs. 3, 5, 6, H–M, Tab. 4)   
 Diagnosis: Morphologically and ecologically similar to T. edaphica differing in the colorless sheath, wider filaments and trichomes, conical end cells, longer Box-B and D1-D1’ helices; structure of V3 helix in the position 9-50 and of basal stem and terminal loop in V2. Differs from T. circinata in Box-B and V3 helices length, the structure in the D1- D1’, Box-B and V3 helices, as well as the color of thallus, rare false branching, wider trichomes, parallel thylakoids and presence of necridia.   
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 Description: Thallus radially spreading, expanded, leathery, brownish, dark olive, blue-green to light greenish. Filaments long, variously curved, (3.0) 3.8-4.9 (6.0) μm wide. False branching rare, mostly double. Sheath colourless, firm, thick. Trichomes slightly constricted at cross-walls, occasionally becoming almost biseriate due to oblique division and compression of cells, 2.2–3.3 μm wide. Cells cylindrical, isodiametric to longer than wide, with parietal thylakoids, 2.2–3.5 μm long. End cells rounded to conical. Necridia in both young and senescing trichomes, hormogonia rare. D1- D1’ helix 85 nucleotides long, with a 3’ unilateral bulge at position –/ 73-80, five internal loops at position 10/68, 14- 15/63-64, 20/58, 23/54-55 and 31/46. Terminal loop having sequence 5’-UCUC-3’. Box B helix 48 nucleotides long, with several assymetrical internal loops at position 6/41–42, 9–10/39 and –/30–32. Terminal loop 5 nucleotides long, 5’-AUAAU-3’. V2 helix 10 nucleotides long, terminal loop 4 nucleotides, 5’-AUUA-3’. V3 helix 60 nucleotides long, with several internal loops at positions 4/53–55, 9–10/47–50 and 23/34. Terminal loop having sequence 5’-GAAA-3’.   
 Etymology: Named for its occurrence in biocrusts on dunes; dunensis (L) = of dunes.   
 Holotype here designated: KW-A-32531, Algotheca, Herbarium of the M.G. Kholodny Institute of Botany of the National Academy of Sciences of Ukraine (KW), Kyiv, Ukraine.   
 Reference strain: Us-6-3 was deposited at the German Collection for Microorganisms and Cell Cultures (DSM 112875), Braunschweig, Germany, as well as at culture collection of the M.G. Kholodny Institute of Botany of the National Academy of Sciences of Ukraine (IBASU-A-754).   
 Type locality: The coast of the Baltic Sea, Usedom Island, vicinity of Zempin, Germany, 54°04′17″ N 13°58′04″ E, collected on 8 October 2013 by Tatiana Mikhailyuk. Sand dunes, biocrusts with dominance of cyanobacteria and streptophyte algae.
description: Published as part of Mikhailyuk, Tatiana, Vinogradova, Oksana, Holzinger, Andreas, Glaser, Karin, Akimov, Yuri & Karsten, Ulf, 2022, Timaviella dunensis sp. nov. from sand dunes of the Baltic Sea, Germany, and emendation of Timaviella edaphica (Elenkin) O. M. Vynogr. & Mikhailyuk (Synechococcales, Cyanobacteria) based on an integrative approach, pp. 192-208 in Phytotaxa 532 (3) on pages 198-205, DOI: 10.11646/phytotaxa.532.3.1, http://zenodo.org/record/5964572