cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Participant experiences of eight weeks of supervised or home-based Pilates among people with multiple sclerosis: a qualitative analysis
Creators: name: Fleming, Karl M.
name: Herring, Matthew P.
name: Coote, Susan B.
name: Tindall, Daniel
Publisher: name: Taylor & Francis
Year: 2021
Subjects: subject: Microbiology
subject: FOS: Biological sciences
subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: Science Policy

description: An exploratory qualitative study investigating participants’ experiences of a feasibility study of supervised and home-based Pilates. All 10 females who participated in an eight-week supervised (n = 4) or home-based Pilates (n = 6) program were invited and agreed to be interviewed. Data were collected via semi-structured interviews and researcher observations of participant experiences. Data were analysed using codebook thematic analysis framework. Two domain summaries emerged: (1) the suitability of home-based Pilates in particular for this population. Participants indicated that supervised and home-based Pilates was a safe, intensity-appropriate, and implementable exercise method to improve mental health outcomes among persons with multiple sclerosis (PwMS), and (2) the benefits experienced by PwMS while participating in Pilates. Home-based Pilates reduced both exercise participation and accessibility barriers commonly experienced by PwMS. Furthermore, PwMS reported improvements in mental health outcomes following Pilates, in both exercise environments. PwMS reported experiencing improved mood following regular Pilates training and indicated that home-based in particular reduced barriers regularly experienced by this population. Results support Pilates as a feasible exercise modality providing potential mood improvements among PwMS. Future appropriately powered home-based randomised controlled trials to further explore the effects of Pilates training on mental health among PwMS with minimal-to-mild mobility disability are warranted.Implications for RehabilitationParticipants in this study described the mental health benefits experienced from engaging in Pilates, a non-traditional exercise modality.Home-based Pilates overcame participatory and accessibility barriers to exercise among people with multiple sclerosis (MS).Participants valued that Pilates was low intensity and did not exacerbate fatigue.Home-based Pilates is a feasible exercise method for people with MS with minimal-to-mild mobility disability. Participants in this study described the mental health benefits experienced from engaging in Pilates, a non-traditional exercise modality. Home-based Pilates overcame participatory and accessibility barriers to exercise among people with multiple sclerosis (MS). Participants valued that Pilates was low intensity and did not exacerbate fatigue. Home-based Pilates is a feasible exercise method for people with MS with minimal-to-mild mobility disability.
Molecular analysis of dUTPase of Helicobacter pylori for identification of novel inhibitors using in silico studies
Creators: name: Sisodia, Rinki
name: Sarmadhikari, Debapriyo
name: Mazumdar, Pooja Anjali
name: Asthana, Shailendra
name: Madhurantakam, Chaithanya
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Biophysics
subject: Biochemistry
subject: Space Science
subject: Medicine
subject: Pharmacology
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Information Systems not elsewhere classified
subject: Cancer
subject: Infectious Diseases
subject: FOS: Health sciences

description: The human gastric pathogen Helicobacter pylori chronically affects the gastric mucosal layer of approximately half of world’s population. The emergence of resistant strains urges the need for identification of novel and selective drug against new molecular targets. A ubiquitous enzyme, Deoxyuridine 5’-triphosphate nucleotidohydrolase (dUTPase), is considered as first line of defense against uracil mis-incorporation into DNA, and essential for genome integrity. Lack of dUTPase triggers an elevated recombination frequency, DNA breaks and ultimately cell death. Hence, dUTPase can be considered as a promising target for development of novel lead inhibitor compounds in H. pylori treatment. Herein, we report the generation of three-dimensional model of the target protein using comparative modelling and its validation. To identify dUTPase inhibitors, a high throughput virtual screening approach utilizing Knowledge-based inhibitors and DrugBank database was implemented. Top ranked compounds were scrutinized based on investigations of the protein-ligand interaction fingerprints, molecular interaction maps and binding affinities and the drug potentiality. The best ligands were studied further for complex stability and intermolecular interaction profiling with respect to time under 100 ns classical molecular dynamic stimulation, establishing significant stability in dynamic states as observed from RMSD and RMSF parameters and interactions with the catalytic site residues. The binding free energy calculation computed using MM-GBSA method from the MD simulation trajectories demonstrated that our molecules possess strong binding affinity towards the Helicobacter pylori dUTPase protein. We conclude that our proposed molecules may be potential lead molecules for effective inhibition against the H. pylori dUTPase protein subject to experimental validation. Communicated by Ramaswamy H. Sarma
A dominant positive and negative selectable gene for use in mammalian cells.
Creators: name: Hickey, R.
name: Kucherlapati, R.
name: Skoultchi, A.
name: Schwartz, F.
name: Smithies, O.
name: Maeda, N.
name: Edelmann, W.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1991

description: We have constructed three different fusion genes containing the herpes simplex virus thymidine kinase (HSV tk) and the bacterial neomycin phosphotransferase (neo) genes. All three fusion genes utilize the HSV tk promoter but differ at the junction of their components. We have determined if the fusion genes are bifunctional by introducing them into mammalian cells and testing for function of the individual components. One of the fusion genes, TNFUS 69, produced a bicistronic message and a fusion protein that has TK and NEO protein functions. This and other fusion genes of a similar nature could serve as dominant positive and negative selectable markers in mammalian cells.
The Calibration of the WISE W1 and W2 Tully-Fisher Relation
Creators: name: Neill, J. D.
name: Seibert, Mark
name: Tully, R. Brent
name: Courtois, Hélène
name: Sorce, Jenny G.
name: Jarrett, T. H.
name: Scowcroft, Victoria
name: Masci, Frank J.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Cosmology and Nongalactic Astrophysics (astro-ph.CO)
subject: Astrophysics of Galaxies (astro-ph.GA)
subject: FOS: Physical sciences

description: In order to explore local large-scale structures and velocity fields, accurate galaxy distance measures are needed. We now extend the well-tested recipe for calibrating the correlation between galaxy rotation rates and luminosities -- capable of providing such distance measures -- to the all-sky, space-based imaging data from the Wide-field Infrared Survey Explorer (WISE) W1 ($3.4μ$m) and W2 ($4.6μ$m) filters. We find a linewidth to absolute magnitude correlation (known as the Tully-Fisher Relation, TFR) of $\mathcal{M}^{b,i,k,a}_{W1} = -20.35 - 9.56 (\log W^i_{mx} - 2.5)$ (0.54 magnitudes rms) and $\mathcal{M}^{b,i,k,a}_{W2} = -19.76 - 9.74 (\log W^i_{mx} - 2.5)$ (0.56 magnitudes rms) from 310 galaxies in 13 clusters. We update the I-band TFR using a sample 9% larger than in Tully & Courtois (2012). We derive $\mathcal{M}^{b,i,k}_I = -21.34 - 8.95 (\log W^i_{mx} - 2.5)$ (0.46 magnitudes rms). The WISE TFRs show evidence of curvature. Quadratic fits give $\mathcal{M}^{b,i,k,a}_{W1} = -20.48 - 8.36 (\log W^i_{mx} - 2.5) + 3.60 (\log W^i_{mx} - 2.5)^2$ (0.52 magnitudes rms) and $\mathcal{M}^{b,i,k,a}_{W2} = -19.91 - 8.40 (\log W^i_{mx} - 2.5) + 4.32 (\log W^i_{mx} - 2.5)^2$ (0.55 magnitudes rms). We apply an I-band -- WISE color correction to lower the scatter and derive $\mathcal{M}_{C_{W1}} = -20.22 - 9.12 (\log W^i_{mx} - 2.5)$ and $\mathcal{M}_{C_{W2}} = -19.63 - 9.11 (\log W^i_{mx} - 2.5)$ (both 0.46 magnitudes rms). Using our three independent TFRs (W1 curved, W2 curved and I-band), we calibrate the UNION2 supernova Type Ia sample distance scale and derive $H_0 = 74.4 \pm 1.4$(stat) $\pm\ 2.4$(sys) kms$^{-1}$ Mpc$^{-1}$ with 4% total error.
description: 22 page, 21 figures, accepted to ApJ, Table 1 data at http://spartan.srl.caltech.edu/~neill/tfwisecal/table1.txt
A 3D copper (II) coordination polymer based on sulfanilic acid ligand (CP 1) for efficient biomolecular interaction with bovine serum albumin: spectroscopic, molecular modelling and DFT analysis
Creators: name: Firdaus, Shama
name: Amir, Mohd
name: Ahmad, Aiman
name: Ali, Arif
name: Alam, Mohammad Jane
name: Dilshad, Sumra
name: Javed, Saleem
name: Ahmad, Musheer
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Biophysics
subject: Biochemistry
subject: Physical Sciences not elsewhere classified
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Chemical Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified

description: Several biochemical reactions occur during the interaction of metal complexes and proteins due to conformational modifications in the structure of the protein, which provide fundamental knowledge of the effect, mechanism, and transport of many drugs throughout the body. Here, we report the synthesis, identification, and impact of the 3-dimensional Copper(II)sulfanilic acid coordination polymer (CP 1) on interactions with bovine serum albumin (BSA). The CP 1 was synthesized via a simple hot stirring method, and the single crystal XRD confirms the effective bonding interactions between metal and organic ligand, forming a crystalline polymeric chain and the topological study shows the sql type of underlying net topology. Powder XRD, Fourier transform infrared spectroscopy, and thermogravimetric analysis were also performed. Moreover, DFT/B3LYP calculations provide chemical precision for the resulting complex. Further, the changes that occur in the secondary structure of protein when CP 1 binds with BSA as well as its binding capacity were investigated via circular dichroism analysis and spectroscopic methods such as UV-absorption spectroscopy and fluorescence spectroscopy, respectively. The CP 1/BSA complex melting point was also measured, and its temperature-dependent heat denaturation was studied along with molecular docking. Communicated by Ramaswamy H. Sarma
Jesus - eine nicht reduzierbare Vielfalt von Erzählungen
Creators: name: Bingemer, Maria Clara Lucchetti
name: Borgmann, Erik
name: Cahill, Lisa Sowle
name: Torres Queiruga, Andrés
Publisher: name: Concilium
Year: 1970

description: Concilium, Bd. 44 Nr. 3 (2008): Concilium: Internationale Zeitschrift für Theologie, Band 44, Heft 3
Psychometric properties and cross-cultural adaptation of the Patient-Rated Tennis Elbow Evaluation (PRTEE); a systematic review and meta-analysis
Creators: name: Shafiee, Erfan
name: MacDermid, Joy C.
name: Walton, David
name: Vincent, Joshua I.
name: Grewal, Ruby
Publisher: name: Taylor & Francis
Year: 2021
Subjects: subject: Medicine
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: To synthesize and appraise the evidence on the translation, cross-cultural adaptation, and psychometric properties of the Patient-Rated Tennis Elbow Evaluation (PRTEE). Five electronic databases were searched from 1999 to 2021. Two independent reviewers assessed the risk of bias and quality of the included studies using the COSMIN recommended guidelines. The overall quality of the evidence on each psychometric property was provided using the GRADE approach. Twenty-one studies met the criteria for inclusion in this review. The PRTEE was translated and cross-culturally adapted to 13 languages and cultures. High quality of evidence supports sufficient pooled results for test-retest reliability (0.96(CI: 0.94–0.97)), construct validity (against Disabilities of the Arm, Shoulder, and Hand (DASH): 0.81(CI: 0.76–0.85), against Visual Analogue Scale (VAS): 0.70 (0.62–0.74)), and responsiveness of the PRTEE. The overall rating of the structural validity and internal consistency (0.96(CI: 0.94–0.97)) was indeterminate, and the quality of evidence was low and moderate, respectively. Regarding measurement error, the overall rating of the summarized results (Standard error of measurement (SEM): 3.1 (1.8 to 4.4) and Minimal Detectable Change (MDC95): 8.9 (5.3–12.5)) was sufficient, and the quality of evidence was moderate. Moderate to high quality evidence supports adequate psychometric properties of the PRTEE in terms of internal consistency, test-retest reliability, measurement error, hypothesis testing for construct validity, and responsiveness.Implications for RehabilitationThe PRTEE can measure the construct of pain and disability at a single time-point and detect changes over time precisely.The PRTEE can be used in clinical setting for patients with acute, subacute, and chronic Lateral Elbow Tendinopathy (LET), athletes who suffer from elbow pain and tennis players. The PRTEE can measure the construct of pain and disability at a single time-point and detect changes over time precisely. The PRTEE can be used in clinical setting for patients with acute, subacute, and chronic Lateral Elbow Tendinopathy (LET), athletes who suffer from elbow pain and tennis players.
Master-Slave Locking of Optomechanical Oscillators Over A Long Distance
Creators: name: Shah, Shreyas Y.
name: Zhang, Mian
name: Rand, Richard
name: Lipson, Michal
Publisher: name: arXiv
Year: 2014
Subjects: subject: Optics (physics.optics)
subject: FOS: Physical sciences

description: Frequency-locking and other phenomena emerging from nonlinear interactions between mechanical oscillators are of scientific and technological importance. However, existing schemes to observe such behaviour are not scalable over distance. We demonstrate a scheme to couple two independent mechanical oscillators, separated in frequency by 80kHz and situated far from each other (3.2km), via light. Using light as the coupling medium enables this scheme to have low loss and be extended over long distances. This scheme is reversible and can be generalised for arbitrary network configurations.
Affinity-purified CCAAT-box-binding protein (YEBP) functionally regulates expression of a human class II major histocompatibility complex gene and the herpes simplex virus thymidine kinase gene.
Creators: name: Ting, J. P.
name: Zeleznik-Le, N. J.
name: Azizkhan, J. C.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1991

description: Efficient major histocompatibility complex class II gene expression requires conserved protein-binding promoter elements, including X and Y elements. We affinity purified an HLA-DRA Y-element (CCAAT)-binding protein (YEBP) and used it to reconstitute Y-depleted HLA-DRA in vitro transcription. This directly demonstrates a positive functional role for YEBP in HLA-DRA transcription. The ability of YEBP to regulate divergent CCAAT elements was also assessed; YEBP was found to partially activate the thymidine kinase promoter. This functional analysis of YEBP shows that this protein plays an important role in the regulation of multiple genes.
cDNA cloning of an intracellular form of the human interleukin 1 receptor antagonist associated with epithelium.
Creators: name: Fong, S.
name: Morris, J.
name: Hammerberg, C.
name: Jaffe, G. J.
name: Van Le, L.
name: Martin, G.
name: Sporn, S. A.
name: Haskill, S.
name: Bigler, C. F.
name: Peace, A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1991

description: A cDNA encoding a receptor antagonist of interleukin 1 (IL-1ra), secreted from human monocytes, has recently been isolated and sequenced [Eisenberg, S. P., Evans, R. J., Arend, W. P., Verderber, E., Brewer, M. T., Hannum, C. H. & Thompson, R. C. (1990) Nature (London) 343, 341-346]. We have identified another version of this IL-1ra, which is predominantly expressed in epithelial cells. This IL-1ra lacks a leader sequence and, thus, is probably intracellular. Both proteins are derived from the same gene through use of an alternative transcriptional start site and internal splice-acceptor site. Expression of intracellular IL-1ra cDNA in COS cells demonstrated that the intracellular product specifically inhibited exogenous interleukin 1-dependent responses. Keratinocytes were shown to contain significant amounts of nonsecreted IL-1ra protein. Constitutive expression of the intracellular IL-1ra may be an intracellular defensive mechanism in exposed epithelial cells and/or may serve to regulate autocrine interleukin 1-mediated pathways of differentiation.
Rivelazione di neutrini di alta energia da sorgenti puntiformi extra-galattiche con l'apparato Cherenkov sottomarino NESTOR
Creators: name: Lucarelli, Fabrizio
Publisher: name: Zenodo
Year: 1998

description: Negli ultimi anni gli esperimenti sui raggi cosmici hanno rivelato l'esistenza di particelle cosmiche con energie superiori ai 1020 eV. Allo stato attuale non abbiamo nessun indizio sulla loro provenienza o su come queste particelle possano venir accelerate a queste energie. I raggi cosmici di più alta energia sono certamente di origine extra-galattica: in particolare, gettando uno sguardo al di
 fuori della nostra Galassia, i Nuclei Galattici Attivi (AGN) sembrano i più probabili acceleratori di queste particelle estremamente energetiche che giungono fino a noi. L'idea è piuttosto affascinante, anche perchè gli AGN sono la sorgente dei fotoni di più alta energia rivelati tramite rivelatori Cherenkov aerei e tramite osservatori orbitanti. Questa rivelazione ha portato allo sviluppo di vari modelli di emissione di radiazione elettromagnetica dal nucleo di una galassia attiva, i quali tentano di spiegare l'emissione osservata lungo tutto lo spettro, dal radio fino ai raggi gamma; alcuni di questi hanno come conseguenza (o, se vogliamo, come punto di partenza) l'emissione di particelle altamente energetiche (fino a 1019 -- 1020 eV), in particolare protoni e neutrini, di cui i primi potrebbero costituire quella componente di altissima energia dei raggi cosmici che finora è rimasta irrisolta. L'ipotesi che, oltre ai protoni, possano essere emessi anche neutrini di altissima energia, ha portato allo sviluppo della cosiddetta astronomia di neutrini, un nuovo modo di guardare all'Universo che supera, in un certo senso, uno dei problemi principali dell'astronomia classica, e cioè l'assorbimento della radiazione elettromagnetica. La trasparenza della materia ai neutrini, può far si che ci giungano informazioni da regioni opache alla radiazione e può aumentare la nostra conoscenza sulla dinamica dei buchi neri, che potrebbero
 costituire il "motore principale" di un AGN, e su altre problematiche molto attuali, come ad esempio la rivelazione della materia oscura, di supernovae, dei monopoli magnetici etc. Da qui sono nati i progetti dei "telescopi a neutrini", tra i quali NESTOR, che rappresentano una sfida non soltanto sul piano scientifico ma anche e soprattutto su quello tecnologico, vista la necessità di collocarli nelle profondità marine o all'interno del ghiaccio antartico. In questa tesi, viene fatta una stima del numero di eventi di neutrino di altissima energia aspettato da AGN di tipo blazars e viene valutata la capacità del rivelatore NESTOR di rivelare tali sorgenti sul fondo di eventi di muoni indotti dai neutrini atmosferici.
Heterologous hydrogenase overproduction systems for biotechnology—An overview
Creators: name: Fan, Qin
name: Neubauer, Peter
name: Lenz, Oliver
name: Gimpel, Matthias
Publisher: name: Technische Universität Berlin
Year: 2020
Subjects: subject: 570 Biowissenschaften; Biologie
subject: hydrogenases
subject: heterologous expression systems
subject: metalloprotein
subject: biohydrogen
subject: renewable energy
subject: oxygen-tolerance
subject: difficult-to-express protein
subject: in vitro maturation

description: Hydrogenases are complex metalloenzymes, showing tremendous potential as H2-converting redox catalysts for application in light-driven H2 production, enzymatic fuel cells and H2-driven cofactor regeneration. They catalyze the reversible oxidation of hydrogen into protons and electrons. The apo-enzymes are not active unless they are modified by a complicated post-translational maturation process that is responsible for the assembly and incorporation of the complex metal center. The catalytic center is usually easily inactivated by oxidation, and the separation and purification of the active protein is challenging. The understanding of the catalytic mechanisms progresses slowly, since the purification of the enzymes from their native hosts is often difficult, and in some case impossible. Over the past decades, only a limited number of studies report the homologous or heterologous production of high yields of hydrogenase. In this review, we emphasize recent discoveries that have greatly improved our understanding of microbial hydrogenases. We compare various heterologous hydrogenase production systems as well as in vitro hydrogenase maturation systems and discuss their perspectives for enhanced biohydrogen production. Additionally, activities of hydrogenases isolated from either recombinant organisms or in vivo/in vitro maturation approaches were systematically compared, and future perspectives for this research area are discussed.
Direct observation of brownian motion of lipids in a membrane.
Creators: name: Lee, G. M.
name: Jacobson, K. A.
name: Ishihara, A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1991

description: Nanovid microscopy, which uses 30- to 40-nm colloidal gold probes combined with video-enhanced contrast, can be used to examine random and directed movements of individual molecules in the plasma membrane of living cells. To validate the technique in a model system, the movements of lipid molecules were followed in a supported, planar bilayer containing fluorescein-conjugated phosphatidylethanolamine (Fl-PtdEtn) labeled with 30-nm gold anti-fluorescein (anti-Fl). Multivalent gold probes were prepared by conjugating only anti-Fl to the gold. Paucivalent probes were prepared by mixing an irrelevant antibody with the anti-Fl prior to conjugation. The membrane-bound gold particles moved in random patterns that were indistinguishable from those produced by computer simulations of two-dimensional random motion. The multivalent gold probes had an average lateral diffusion coefficient (D) of 0.26 x 10(-8) cm2/sec, and paucivalent probes had an average D of 0.73 x 10(-8) cm2/sec. Sixteen percent of the multivalent and 50% of the paucivalent probes had values for D in excess of 0.6 x 10(-8) cm2/sec, which, after allowance for stochastic variation, are consistent with the D of 1.3 x 10(-8) cm2/sec measured by fluorescence recovery after photobleaching of Fl-PtdEtn in the planar bilayer. The effect of valency on diffusion suggests that the multivalent gold binds several lipids forming a disk up to 30-40 nm in diameter, resulting in reduced diffusion with respect to the paucivalent gold, which binds one or a very few lipids. Provided the valency of the gold probe is considered in the interpretation of the results. Nanovid microscopy is a valid method for analyzing the movements of single or small groups of molecules within membranes.
Early English genealogies : the evolution of their content, form, and function
Creators: name: Eddington, Christopher Mark
Publisher: name: The University of St Andrews
Year: 2022
Subjects: subject: Genealogies
subject: Royal
subject: Biblical influence
subject: Early medieval English
subject: Alliterating
subject: Fourteen-generation structure
subject: Woden
subject: Geota/Geata
subject: Cerdic
subject: Dynastic pedigrees
subject: PN56.G46E3
subject: Genealogical literature--England--History--To 1500
subject: Genealogy in literature
subject: Bible--Genealogy

description: This thesis traces, for the first time in detail, the evolution of early English genealogical literary forms in pre-Conquest texts, from the short pedigrees written in Latin in Historia ecclesiastica gentis Anglorum in the early eighth century to the extensive pedigree of Æthelwulf in the Anglo-Saxon Chronicle in the late ninth. It argues that early English genealogies were modelled on those in the Bible, and influenced by theological number symbolism and chronological frameworks, which were based on biblical genealogy. Key characteristics of English genealogy are discussed including: descent from Woden; the use of ethnonyms and dynastic eponyms; the alliteration of names, and the formal properties of structures and patterns in genealogies. A central argument of this thesis is that many of the variations between the content of different versions of shared or similar genealogical materials result from the increasing importance to writers of their structural, alliterative, and metrical forms, the development of which reflects, or even affects, the changing priorities or ideologies of the genealogists. As almost all of the genealogies are incorporated into other texts, the purposes of those texts are considered and the use the genealogies are put to. A second key argument of this thesis is that genealogy performs ideological work within narrative and other literary texts
DNA-binding protein RAP1 stimulates meiotic recombination at the HIS4 locus in yeast.
Creators: name: White, M. A.
name: Wierdl, M.
name: Petes, T. D.
name: Detloff, P.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1991

description: In the yeast Saccharomyces cerevisiae, as in other eukaryotes, some regions of the genome have a much higher rate of meiotic recombination than others. We show below that the binding of the RAP1 protein to a site upstream of the HIS4 gene is necessary for a high rate of meiotic (but not mitotic) recombination at this locus. A mutation in the RAP1 binding site at HIS4 results in a decrease in recombination; overproduction of RAP1 causes an increase in recombination at HIS4 above wild-type levels
Delineating multi-epitopes vaccine designing from membrane protein CL5 against all monkeypox strains: a pangenome reverse vaccinology approach
Creators: name: Alsaiari, Ahad Amer
name: Hakami, Mohammed Ageeli
name: Alotaibi, Bader S.
name: Alkhalil, Samia S.
name: Alkhorayef, Nada
name: Khan, Kanwal
name: Jalal, Khurshid
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Genetics
subject: Biotechnology
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Information Systems not elsewhere classified
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Virology
subject: Computational Biology

description: The recently identified monkeypox virus (MPXV or mpox) is a zoonotic orthopox virus that infects humans and causes diseases with traits like smallpox. The world health organization (WHO) estimates that 3-6% of MPXV cases result in death. As it might impact everyone globally, like COVID, and become the next pandemic, the cure for this disease is important for global public health. The high incidence and disease ratio of MPXV necessitates immediate efforts to design a unique vaccine candidate capable of addressing MPXV diseases. Here, we used a computational pan-genome-based vaccine design strategy for all currently reported 19 MPXV strains acquired from different regions of the world. Thus, this study's objective was to develop a new and safe vaccine candidate against MPXV by targeting the membrane CL5 protein; identified after the pangenome analysis. Proteomics and reverse vaccinology have covered up all of the MPXV epitopes that would usually stimulate robust host immune responses. Following this, only two mapped (MHC-I, MHC-II, and B-cell) epitopes were observed to be extremely effective that can be used in the construction of CL5 protein vaccine candidates. The suggested vaccine (V5) candidate from eight vaccine models was shown to be antigenic, non-allergenic, and stable (with 213 amino acids). The vaccine's candidate efficacy was evaluated by using many in silico methods to predict, improve, and validate its 3D structure. Molecular docking and molecular dynamics simulations further reveal that the proposed vaccine candidate ensemble has a high interaction energy with the HLAs and TRL2/4 immunological receptors under study. Later, the vaccine sequence was used to generate an expression vector for the E. coli K12 strain. Further study uncovers that V5 was highly immunogenic because it produced robust primary, secondary, and tertiary immune responses. Eventually, the use of computer-aided vaccine designing may significantly reduce costs and speed up the process of developing vaccines. Although, the results of this research are promising, however, more research (experimental; in vivo, and in vitro studies) is needed to verify the biological efficacy of the proposed vaccine against MPXV. Communicated by Ramaswamy H. Sarma
Optimization of Culture Conditions for Oxygen-Tolerant Regulatory [NiFe]-Hydrogenase Production from Ralstonia eutropha H16 in Escherichia coli
Creators: name: Fan, Qin
name: Caserta, Giorgio
name: Lorent, Christian
name: Lenz, Oliver
name: Neubauer, Peter
name: Gimpel, Matthias
Publisher: name: Technische Universität Berlin
Year: 2021
Subjects: subject: 570 Biowissenschaften; Biologie
subject: [NiFe]-hydrogenase
subject: Ralstonia eutropha
subject: heterologous protein production
subject: cofactor assembly
subject: difficult-to-express protein
subject: Escherichia coli

description: Hydrogenases are abundant metalloenzymes that catalyze the reversible conversion of molecular H2 into protons and electrons. Important achievements have been made over the past two decades in the understanding of these highly complex enzymes. However, most hydrogenases have low production yields requiring many efforts and high costs for cultivation limiting their investigation. Heterologous production of these hydrogenases in a robust and genetically tractable expression host is an attractive strategy to make these enzymes more accessible. In the present study, we chose the oxygen-tolerant H2-sensing regulatory [NiFe]-hydrogenase (RH) from Ralstonia eutropha H16 owing to its relatively simple architecture compared to other [NiFe]-hydrogenases as a model to develop a heterologous hydrogenase production system in Escherichia coli. Using screening experiments in 24 deep-well plates with 3 mL working volume, we investigated relevant cultivation parameters, including inducer concentration, expression temperature, and expression time. The RH yield could be increased from 14 mg/L up to >250 mg/L by switching from a batch to an EnPresso B-based fed-batch like cultivation in shake flasks. This yield exceeds the amount of RH purified from the homologous host R. eutropha by several 100-fold. Additionally, we report the successful overproduction of the RH single subunits HoxB and HoxC, suitable for biochemical and spectroscopic investigations. Even though both RH and HoxC proteins were isolated in an inactive, cofactor free apo-form, the proposed strategy may powerfully accelerate bioprocess development and structural studies for both basic research and applied studies. These results are discussed in the context of the regulation mechanisms governing the assembly of large and small hydrogenase subunits.
A systematic review of self-determination interventions for children and youth with disabilities
Creators: name: Lindsay, Sally
name: Varahra, Azar
Publisher: name: Taylor & Francis
Year: 2021
Subjects: subject: Medicine
subject: Neuroscience
subject: Physiology
subject: FOS: Biological sciences
subject: Pharmacology
subject: Biotechnology
subject: Sociology
subject: FOS: Sociology
subject: Science Policy

description: Developing self-determination skills is an important component of youth’s maturation. However, many youths with disabilities lack opportunities to engage in activities that can promote these skills. The purpose of this study was to conduct a systematic review of self-determination interventions for children and youth with disabilities. A systematic review was performed on seven international databases that identified 28 studies meeting our inclusion criteria. Among the 28 studies, 5184 youth with disabilities (aged 9–29, mean age 16.8 years) were represented across three countries. The interventions varied in duration, length, number of sessions, and delivery format, which included curriculum-based, workshops, experiential or residential learning, peer coaching and mentoring, self-directed individual learning, computer games, and multi-component designs. 19/28 studies reported a significant improvement in self-determination. Of these 13/18 studies showed improvements as measured by Arc’s self-determination scale, while 8/13 studies reported a significant improvement as measured by AIR self-determination scale. Other significant differences were reported in vocational skills self-efficacy and self-determination inventory. Our findings highlight that there are several types of interventions that have the potential to improve self-determination for youth with disabilities. Further research is needed to understand what program components could help youth to obtain optimal outcomes.Implications for RehabilitationSeveral types of interventions (school-based, community, online) have potential to influence self-determination for youth with disabilities.Clinicians and educators should consider having multiple components including workshops, one-to-one and group-based learning, mentoring and coaching for optimal program outcomes.Interventions that are targeted to enhance self-determination for youth with disabilities should be theoretically informed and use a validated measure to assess their effectiveness. Several types of interventions (school-based, community, online) have potential to influence self-determination for youth with disabilities. Clinicians and educators should consider having multiple components including workshops, one-to-one and group-based learning, mentoring and coaching for optimal program outcomes. Interventions that are targeted to enhance self-determination for youth with disabilities should be theoretically informed and use a validated measure to assess their effectiveness.
Effect of primordial non-Gaussianities on the far-UV luminosity function of high-redshift galaxies: implications for cosmic reionization
Creators: name: Chevallard, Jacopo
name: Silk, Joseph
name: Nishimichi, Takahiro
name: Habouzit, Melanie
name: Mamon, Gary A.
name: Peirani, Sébastien
Publisher: name: arXiv
Year: 2014
Subjects: subject: Cosmology and Nongalactic Astrophysics (astro-ph.CO)
subject: FOS: Physical sciences

description: [Abridged] Understanding how the intergalactic medium (IGM) was reionized at z > 6 is one of the big challenges of current high redshift astronomy. It requires modelling the collapse of the first astrophysical objects (Pop III stars, first galaxies) and their interaction with the IGM, while at the same time pushing current observational facilities to their limits. The observational and theoretical progress of the last few years have led to the emergence of a coherent picture in which the budget of hydrogen-ionizing photons is dominated by low-mass star-forming galaxies, with little contribution from Pop III stars and quasars. The reionization history of the Universe therefore critically depends on the number density of low-mass galaxies at high redshift. In this work, we explore how changes in the statistical properties of initial density fluctuations affect the formation of early galaxies. Following Habouzit et al. (2014), we run 5 N-body simulations with Gaussian and (scale-dependent) non-Gaussian initial conditions, all consistent with Planck constraints. By appealing to a galaxy formation model and to a population synthesis code, we compute the far-UV galaxy luminosity function down to M_UV = -14 at redshift 7 < z < 15. We find that models with strong primordial non-Gaussianities on < Mpc scales show a far-UV luminosity function significantly enhanced in low-mass galaxies. We adopt a reionization model calibrated from state-of-the-art hydrodynamical simulations and show that such non-Gaussianities leave a clear imprint on the Universe reionization history and electron Thomson scattering optical depth tau_E. Although current uncertainties in the physics of reionization and on the determination of tau_E still dominate the signatures of non-Gaussianities, our results suggest that tau_E could ultimately be used to constrain the statistical properties of initial density fluctuations.
description: 18 pages, 12 figures, accepted for publication in MNRAS
Production of soluble regulatory hydrogenase from Ralstonia eutropha in Escherichia coli using a fed-batch-based autoinduction system
Creators: name: Fan, Qi
name: Neubauer, Peter
name: Gimpel, Matthias
Publisher: name: Technische Universität Berlin
Year: 2021
Subjects: subject: 540 Chemie und zugeordnete Wissenschaften
subject: Escherichia coli
subject: ralstonia eutropha
subject: difficult-to-express protein
subject: EnPresso B
subject: fed-batch like cultivation
subject: IPTG autoinduction
subject: lactose autoinduction
subject: regulatory hydrogenase

description: Background: Autoinduction systems can regulate protein production in Escherichia coli without the need to monitor cell growth or add inducer at the proper time following culture growth. Compared to classical IPTG induction, autoinduction provides a simple and fast way to obtain high protein yields. In the present study, we report on the optimization process for the enhanced heterologous production of the Ralstonia eutropha regulatory hydrogenase (RH) in E. coli using autoinduction. These autoinduction methods were combined with the EnPresso B fed-batch like growth system, which applies slow in situ enzymatic glucose release from a polymer to control cell growth and protein synthesis rate. Results: We were able to produce 125 mg L−1 RH corresponding to a productivity averaged over the whole process time of 3 mg (L h)−1 in shake flasks using classic single-shot IPTG induction. IPTG autoinduction resulted in a comparable volumetric RH yield of 112 mg L−1 and due to the shorter overall process time in a 1.6-fold higher productivity of 5 mg (L h)−1. In contrast, lactose autoinduction increased the volumetric yield more than 2.5-fold and the space time yield fourfold reaching 280 mg L−1 and 11.5 mg (L h)−1, respectively. Furthermore, repeated addition of booster increased RH production to 370 mg L−1, which to our knowledge is the highest RH concentration produced in E. coli to date. Conclusions: The findings of this study confirm the general feasibility of the developed fed-batch based autoinduction system and provide an alternative to conventional induction systems for efficient recombinant protein production. We believe that the fed-batch based autoinduction system developed herein will favor the heterologous production of larger quantities of difficult-to-express complex enzymes to enable economical production of these kinds of proteins.
Cosmological Implications of High-Energy Neutrino Emission from the Decay of Long-Lived Particle
Creators: name: Ema, Yohei
name: Jinno, Ryusuke
name: Moroi, Takeo
Publisher: name: arXiv
Year: 2014
Subjects: subject: High Energy Physics - Phenomenology (hep-ph)
subject: Cosmology and Nongalactic Astrophysics (astro-ph.CO)
subject: High Energy Astrophysical Phenomena (astro-ph.HE)
subject: FOS: Physical sciences

description: We study cosmological scenarios in which high-energy neutrinos are emitted from the decay of long-lived massive particles at the cosmic time later than a redshift of 10^6. The high-energy neutrino events recently observed by the IceCube experiment suggest a new source of high-energy cosmic-ray neutrinos; decay of a heavy particle can be one of the possibilities. We calculate the spectrum of the high-energy neutrinos emitted from the decay of long-lived particles, taking account of the neutrino scattering processes with background neutrinos. Then, we derive bounds on the scenario using the observation of high-energy cosmic-ray neutrino flux. We also study constraints from the spectral distortions of the cosmic microwave background and the big-bang nucleosynthesis. In addition, we show that the PeV neutrinos observed by the IceCube experiment can originate from the decay of a massive particle with its mass as large as O(10^10 GeV).
description: 22 pages, 7 figures
Do interhelical side chain-backbone hydrogen bonds participate in formation of leucine zipper coiled coils?
Creators: name: Kizer, J. S.
name: Tropsha, A.
name: Bowen, J. P.
name: Brown, F. K.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1991

description: The leucine zipper proteins are a group of transcriptional regulators that dimerize to form a DNA binding domain. It has been proposed that this dimerization results from the hydrophobic association of the alpha-helices of two leucine zipper monomers into a coiled coil. We propose a model for a coiled coil based on a periodic hydrophobic-hydrophilic amino acid motif found in the leucine zipper regions of 11 transcriptional regulatory proteins. This model predicts the symmetrical formation of secondary hydrogen bonds between the polar side chains of one helix and the peptide carbonyls of the opposite chain, supplementing the interactions between hydrophobic side chains. Physical modeling (CPK) and in vacuo molecular mechanics calculations of the stability of the GCN4 leucine zipper coiled coil configured in accordance with this model demonstrate a greater stability for this conformer than for a conformer configured according to a current hydrophobic model. Molecular dynamics simulations show similar stability of the two models in vacuo but a higher stability of the hydrophobic model in water.
Monnieriside A from Evolvulus linarioides promotes antinociceptive effects in models of inflammatory and postoperative pain in male mice
Creators: name: de Agnelo-Silva, Diego Francisco
name: Opretzka, Luíza Carolina França
name: Viana, Max Denisson Mauricio
name: Lauria, Pedro Santana Sales
name: Pereira, Laiane Caline Oliveira
name: Abreu, Lucas Silva
name: Tavares, Josean Fechine
name: da Silva, Marcelo Sobral
name: Soares, Milena Botelho Pereira
name: Villarreal, Cristiane Flora
Publisher: name: Taylor & Francis
Year: 2024
Subjects: subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Genetics
subject: Neuroscience
subject: Pharmacology
subject: Environmental Sciences not elsewhere classified
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Science Policy

description: Monnieriside A (MoA) is a chromone isolated from Evolvulus linarioides. This study investigated the antinociceptive potential of MoA in mice. MoA (0.01–100 mg/kg, i.p.) inhibited nociception in the inflammatory phase of the formalin test without causing motor impairment. MoA (0.1–100 mg/kg, i.p.) also reduced hindpaw mechanical allodynia caused by either intraplantar injection of Complete Freund’s Adjuvant (CFA) or surgical paw incision to simulate postoperative pain. Postoperative antinociception was accompanied by reduced IL-1β levels in the incised paw, assessed by ELISA. The antinociceptive action of MoA (100 mg/kg, i.p.) was preserved in IL-10 knockout mice submitted to paw incision, indicating that IL-10 is not essential to the antinociceptive effect. Interestingly, MoA (100 mg/kg, i.p.) increased the expression of TGF-β in IL-10 knockout mice, which could be a compensation mechanism leading to an antinociceptive effect in the absence of IL-10.
A 3D copper (II) coordination polymer based on sulfanilic acid ligand (CP 1) for efficient biomolecular interaction with bovine serum albumin: spectroscopic, molecular modelling and DFT analysis
Creators: name: Firdaus, Shama
name: Amir, Mohd
name: Ahmad, Aiman
name: Ali, Arif
name: Alam, Jane
name: Dilshad, Sumra
name: Javed, Saleem
name: Ahmad, Musheer
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Biophysics
subject: Biochemistry
subject: Physical Sciences not elsewhere classified
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Chemical Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified

description: Several biochemical reactions occur during the interaction of metal complexes and proteins due to conformational modifications in the structure of the protein, which provide fundamental knowledge of the effect, mechanism, and transport of many drugs throughout the body. Here, we report the synthesis, identification, and impact of the 3-dimensional Copper(II)sulfanilic acid coordination polymer (CP 1) on interactions with bovine serum albumin (BSA). The CP 1 was synthesized via a simple hot stirring method, and the single crystal XRD confirms the effective bonding interactions between metal and organic ligand, forming a crystalline polymeric chain and the topological study shows the sql type of underlying net topology. Powder XRD, Fourier transform infrared spectroscopy, and thermogravimetric analysis were also performed. Moreover, DFT/B3LYP calculations provide chemical precision for the resulting complex. Further, the changes that occur in the secondary structure of protein when CP 1 binds with BSA as well as its binding capacity were investigated via circular dichroism analysis and spectroscopic methods such as UV-absorption spectroscopy and fluorescence spectroscopy, respectively. The CP 1/BSA complex melting point was also measured, and its temperature-dependent heat denaturation was studied along with molecular docking. Communicated by Ramaswamy H. Sarma