title: Ð Ð¾Ð»Ñ ÑÐ¸Ð¾ÑÐµÐ´Ð¾ÐºÑÐ¸Ð½ÑÐµÐ´ÑÐºÑÐ°Ð·Ñ Ð² ÑÐ¸ÑÑÐµÐ¼Ðµ Ð°Ð½ÑÐ¸Ð¾ÐºÑÐ¸Ð´Ð°Ð½ÑÐ½Ð¾Ð¹ Ð·Ð°ÑÐ¸ÑÑ Ð¿Ð»ÑÑÐ¸Ð¿Ð¾ÑÐµÐ½ÑÐ½ÑÑ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: The role of thioredoxin reductase in the antioxidant protection system of human pluripotent stem cells
Creators: name: ÐÑÑÑÐµÐ², ÐÐ¸ÐºÐ¸ÑÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Ð¿Ð»ÑÑÐ¸Ð¿Ð¾ÑÐµÐ½ÑÐ½ÑÐµ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸
Ð¾ÐºÐ¸ÑÐ»Ð¸ÑÐµÐ»ÑÐ½ÑÐ¹ ÑÑÑÐµÑÑ
Ð°Ð½ÑÐ¸Ð¾ÐºÑÐ¸Ð´Ð°Ð½ÑÐ½Ð°Ñ ÑÐ¸ÑÑÐµÐ¼Ð°
Ð°ÐºÑÐ¸Ð²Ð½ÑÐµ ÑÐ¾ÑÐ¼Ñ ÐºÐ¸ÑÐ»Ð¾ÑÐ¾Ð´Ð°
Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸-ÐºÐ¾Ð´Ð¸ÑÑÐµÐ¼ÑÐµ Ð±Ð¸Ð¾ÑÐµÐ½ÑÐ¾ÑÑ
pluripotent stem cells
oxidative stress
antioxidant system
reactive oxygen species
genetically encoded biosensors

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description: This study is dedicated to investigating the effectiveness of the antioxidant defense system in pluripotent cells and their differentiated progeny under conditions of external oxidative stress, as well as the contribution of the thioredoxin-dependent enzymatic pathway to the functioning of this system. The objectives of this study were addressed using a previously developed flow cytometric technique in our laboratory based on the analysis of hydrogen peroxide (H2O2) dynamics using the genetically-encoded biosensor HyPer. This technique allows for the quantitative assessment of antioxidant system parameters in living cells. As a result, we were able to demonstrate that the efficiency of antioxidant protection in pluripotent stem cells (PSCs) is similar to that in their differentiated progeny. Also, by inhibiting thioredoxin reductase, we identified its important role in providing antioxidant protection in both pluripotent cells and their differentiated progeny, but only under conditions of oxidative stress of low intensity, close to physiological levels. Contrary to the widely accepted hypothesis of a specific redox state in PSCs, the obtained results indicate the similarity in the functioning of antioxidant systems that maintain redox homeostasis in pluripotent and differentiated human cells.