Additional file 1 of Facile synthesis of near-infrared responsive on-demand oxygen releasing nanoplatform for precise MRI-guided theranostics of hypoxia-induced tumor chemoresistance and metastasis in triple negative breast cancer
Creators: name: Zhang, Dong
name: You, Yuanyuan
name: Xu, Yuan
name: Cheng, Qingqing
name: Xiao, Zeyu
name: Chen, Tianfeng
name: Shi, Changzheng
name: Luo, Liangping
Publisher: name: figshare
Year: 2022
Subjects: Space Science
Physical Sciences not elsewhere classified
Medicine
Cell Biology
Pharmacology
Chemical Sciences not elsewhere classified
Marine Biology
Cancer

description: Additional file 1: Figure S1. (A) The C1s XPS peak of O2-PPSi. (B) The C1s XPS peak of O2-PPSiI. Figure S2. The UV-vis-NIR spectrum of ICG, O2-PPSi and O2-PPSiI. Figure S3. The photographs of O2-PPSi and O2-PPSiI. Figure S4. The magnetic properties of O2-PPSiI. Figure S5. Normalized absorbance intensity at λ = 808 nm divided by the characteristic length of the cell (A/L) at varied concentrations of O2-PPSiI. Figure S6. Representative images of PBS and O2-PPSiI after irradiated with NIR laser (808 nm, 2 W cm−2) for 4 min. Figure S7. Temperature changes of ICG and O2-PPSiI solution with NIR laser (2 W cm−2) switch-on and switch-off for 5 cycles. Figure S8. The image of O2-PPSiI before and after NIR laser irradiation. The appearance of bubbles suggested the O2 release from O2-PPSiI after NIR laser irradiation. Figure S9. The gray value of ultrasonography for water and O2-PPSiI nanosystem solution before and after laser irradiation in vitro. Figure S10. The raised temperature of O2-PPSiI under NIR laser treatment was the main contributor of oxygen release from O2-PPSiI. The scale bar = 100. Figure S11. The gray value of ultrasonography for mice before and after treatment with O2-PPSiI nanosystem and laser irradiation. Figure S12. (A-B) The intracellular uptake of O2-PPSil in TNBC cells (MDA-MB-231) and normal breast cells (Hs 578Bst). (C) Effects of RGD and uPA on the intracellular uptake of O2-PPSil in MDA-MB-231 cells (MDA-MB-231). (D)The fluorescence imaging of O2-PPSiI in vivo. Figure S13. The distribution of O2-PPSiI in liver, spleen and kidney quantified by T1 mapping, and significant difference between the groups at the same time point is indicated at P &lt; 0.05 (*) level. Figure S14. Cytotoxicity of O2-PPSiI with and without NIR irradiation against MDA-MB-231 cells. Figure S15. The overproduction of 1O2 and ROS induced by NIR-triggered O2-PPSiI. Figure S16. The separated tumors of each group at the 21 days after the treatment. Figure S17. IVIM-DWI derived D mapping of tumor in each groups before and after the treatment, and significant difference of relative D values (△D) between the Saline and treatment groups is indicated at P &lt; 0.05 (*) or P &lt; 0.001 (**) level. Figure S18. Pearson correlation analysis between MRI-derived parameters and the expression of E-cadherin, vimentin and Snail-Slug, statistically significant correlation coefficient (r) is indicated at P &lt; 0.05 (*) or P &lt; 0.001 (**) level. Figure S19. Pearson correlation analysis between the expression of Snail-Slug/Vimentin and ZEB1, TGF-β and MMP2, statistically significant correlation coefficient (r) is indicated at P &lt; 0.05 (*) or P &lt; 0.001 (**) level. Figure S20. (A) The relative body weight (△Body weight) changes of tumor-bearing mice in each groups at different time points, and (B) the comparison of △Body weight (%) between the Saline and treatment groups at 21 days after treatment. Significant is indicated at P &lt; 0.05 (*) or P &lt; 0.001 (**) level.