title: ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð±ÐµÐ»ÐºÐ° STIM2 Ð² Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ðµ ÑÑÐ°Ð½ÑÐ³ÐµÐ½Ð½ÑÑ Ð¼ÑÑÐµÐ¹ Ð»Ð¸Ð½Ð¸Ð¸ 5FAD
title: Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÑÐºÐ°Ñ ÑÐ°Ð±Ð¾ÑÐ°
titleType: Subtitle
Creators: name: Ð§ÐµÑÐ½ÑÐº, ÐÐ°ÑÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2016
Subjects: Ð±Ð¾Ð»ÐµÐ·Ð½Ñ ÐÐ»ÑÑÐ³ÐµÐ¹Ð¼ÐµÑÐ°
Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð½Ð°Ñ ÑÐ¾ÐºÑÐ¸ÑÐ½Ð¾ÑÑÑ
Ð´ÐµÐ¿Ð¾-ÑÐ¿ÑÐ°Ð²Ð»ÑÐµÐ¼ÑÐ¹ Ð²Ñ Ð¾Ð´ ÐºÐ°Ð»ÑÑÐ¸Ñ
STIM2
AÎ²-Ð±Ð»ÑÑÐºÐ¸
Alzheimer's disease
amyloid toxicity
store-operated calcium entry
AÎ²-plaques

description: Ð ÑÐ°Ð¼ÐºÐ°Ñ Ð½Ð°ÑÑÐ¾ÑÑÐµÐ¹ ÑÐ°Ð±Ð¾ÑÑ ÑÐ¾Ð»Ñ ÑÐ¸Ð³Ð½Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð¿ÑÑÐ¸ STIM2-nSOC Ð±ÑÐ»Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð° Ð² Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ ÐÐ»ÑÑÐ³ÐµÐ¹Ð¼ÐµÑÐ° (ÐÐ), Ð¼ÑÑÐ°Ñ Ð»Ð¸Ð½Ð¸Ð¸ 5FAD, ÑÐºÑÐ¿ÑÐµÑÑÐ¸ÑÑÑÑÐ¸Ñ Ð¾Ð´Ð½Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾ Ð¿ÑÐµÑÐµÐ½Ð¸Ð»Ð¸Ð½Ð¾Ð²ÑÑ Ð¸ Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð½ÑÑ ÑÐ¾ÐºÑÐ¸ÑÐ½Ð¾ÑÑÑ. Ð Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ Ð±ÑÐ»Ð¾ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¾, ÑÑÐ¾ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð±ÐµÐ»ÐºÐ° STIM2 ÑÐ½Ð¸Ð¶Ð°ÐµÑÑÑ Ð² Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ðµ Ð²Ð·ÑÐ¾ÑÐ»ÑÑ 5FAD Ð¼ÑÑÐµÐ¹ (Ð² Ð²Ð¾Ð·ÑÐ°ÑÑÐµ 4 Ð¸ 6 Ð¼ÐµÑÑÑÐµÐ²). ÐÑÐ¾Ð¼Ðµ ÑÐ¾Ð³Ð¾, Ð² ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²Ð¸Ð¸ Ñ Ð¾Ð¿ÑÐ±Ð»Ð¸ÐºÐ¾Ð²Ð°Ð½Ð½ÑÐ¼Ð¸ Ð´Ð°Ð½Ð½ÑÐ¼Ð¸ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð¾Ð´Ð½Ð¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾ Ñ Ð±ÐµÐ»ÐºÐ¾Ð¼ STIM2 ÑÐ½Ð¸Ð¶Ð°ÐµÑÑÑ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ ÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¼Ð°ÑÐºÐµÑÐ°, Ð±ÐµÐ»ÐºÐ° PSD95. ÐÑÐ»Ð¾ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¾, ÑÑÐ¾ Ð³Ð¸Ð¿ÐµÑÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð±ÐµÐ»ÐºÐ° STIM2, Ð²ÑÐ·Ð²Ð°Ð½Ð½Ð°Ñ Ð¸Ð½ÑÐµÐºÑÐ¸ÐµÐ¹ Ð²Ð¸ÑÑÑÐ° Ð² Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿ Ð²Ð·ÑÐ¾ÑÐ»Ð¾Ð¹ Ð¼ÑÑÐ¸, ÑÐ½Ð¸Ð¶Ð°ÐµÑ Ð² ÑÑÐ¸ ÑÐ°Ð·Ð° ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð¾ Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð½ÑÑ Ð±Ð»ÑÑÐµÐº Ð² ÐºÐ¾ÑÐµ Ð³Ð¾Ð»Ð¾Ð²Ð½Ð¾Ð³Ð¾ Ð¼Ð¾Ð·Ð³Ð° 5FAD Ð¼ÑÑÐµÐ¹. Ð¢Ð°ÐºÐ¸Ð¼ Ð¾Ð±ÑÐ°Ð·Ð¾Ð¼, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð¿Ð¾Ð´ÑÐ²ÐµÑÐ¶Ð´Ð°ÑÑ ÑÐ°Ð½ÐµÐµ Ð²ÑÐ´Ð²Ð¸Ð½ÑÑÑÑ Ð³Ð¸Ð¿Ð¾ÑÐµÐ·Ñ, ÑÑÐ¾ Ð°ÐºÑÐ¸Ð²Ð°ÑÐ¸Ñ STIM2-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾Ð³Ð¾ Ð´ÐµÐ¿Ð¾-ÑÐ¿ÑÐ°Ð²Ð»ÑÐµÐ¼Ð¾Ð³Ð¾ Ð²ÑÐ¾Ð´Ð° ÐºÐ°Ð»ÑÑÐ¸Ñ Ð¼Ð¾Ð¶ÐµÑ Ð¸Ð¼ÐµÑÑ ÑÐµÑÐ°Ð¿ÐµÐ²ÑÐ¸ÑÐµÑÐºÐ¸Ð¹ ÑÑÑÐµÐºÑ Ð´Ð»Ñ Ð»ÐµÑÐµÐ½Ð¸Ñ Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ ÐÐ»ÑÑÐ³ÐµÐ¹Ð¼ÐµÑÐ°. Ð ÑÐ°Ð±Ð¾ÑÐµ Ð±ÑÐ»Ð¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð¼ÐµÑÐ¾Ð´Ñ: Ð³ÐµÐ½Ð¾ÑÐ¸Ð¿Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð¼ÑÑÐµÐ¹ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð½Ð¾Ð¹ ÑÐµÐ¿Ð½Ð¾Ð¹ ÑÐµÐ°ÐºÑÐ¸Ð¸, ÐÐµÑÑÐµÑÐ½-ÐÐ»Ð¾Ñ Ð°Ð½Ð°Ð»Ð¸Ð·, ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²ÐµÐ½Ð½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð· ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð±ÐµÐ»ÐºÐ¾Ð² Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ñ QuantityOne, ÑÑÐµÑÐµÐ¾ÑÐ°ÐºÑÐ¸ÑÐµÑÐºÐ°Ñ Ð¸Ð½ÑÐµÐºÑÐ¸Ñ Ð°Ð´ÐµÐ½Ð¾Ð°ÑÑÐ¾ÑÐ¸Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ Ð²Ð¸ÑÑÑÐ¾Ð² Ð² Ð¡Ð1 Ð¾Ð±Ð»Ð°ÑÑÑ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð° Ð²Ð·ÑÐ¾ÑÐ»ÑÑ Ð¼ÑÑÐµÐ¹, Ð¸Ð¼Ð¼ÑÐ½Ð¾Ð³Ð¸ÑÑÐ¾ÑÐ¸Ð¼Ð¸ÑÐµÑÐºÐ¾Ðµ Ð¾ÐºÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ðµ Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð½ÑÑ Ð±Ð»ÑÑÐµÐº Ð² ÑÐ¾Ð½ÐºÐ¸Ñ ÑÑÐµÐ·Ð°Ñ Ð¼Ð¾Ð·Ð³Ð°, ÐºÐ¾Ð½ÑÐ¾ÐºÐ°Ð»ÑÐ½Ð°Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ, ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²ÐµÐ½Ð½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð· Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð½ÑÑ Ð±Ð»ÑÑÐµÐº Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ñ Icy.
description: As part of this work the role of signaling pathways STIM2-nSOC was studied in a model of Alzheimer's disease (AD), 5FAD line of mice, who expressing presenilin and amyloid toxicity. In this work it was found that expression of protein STIM2 decreases in hippocampus of adult 5FAD mice (4 and 6 months). Furthermore, in accordance with published data it shows that expression of synaptic marker, PSD95 protein, decreases at the same time with expression of protein STIM2. It was found that overexpression protein STIM2, induced by injection of virus into the hippocampus of adult mouse, reduces tripled the amount of amyloid plaques in the cerebral cortex of 5FAD mice. Thus, these results confirm the previously hypothesized that activation STIM2-dependent store-operated calcium entry may have a therapeutic effect in the treatment of Alzheimer's disease.In this work the following methods were used: genotyping mice by PCR, Western blot analysis, quantitative analysis of protein expression using QuantityOne program, stereotactic injection of adeno-associated viruses in the CA1 region of the hippocampus of adult mice, immunohistochemical staining of amyloid plaques in thin brain slices, confocal microscopy, quantitative analysis of amyloid plaques using Icy program.