Additional file 1 of Combined metagenomics and metabolomic analysis of microbial community structure and metabolic function in continuous soybean cropping soils of Songnen Plain, China
Creators: name: Xu, Letian
name: Jin, Shun
name: Su, Yue
name: Lyu, Xiaochen
name: Yan, Shuangshuang
name: Wang, Chang
name: Cao, Liang
name: Yan, Chao
name: Ma, Chunmei
Publisher: name: figshare
Year: 2024
Subjects: subject: Microbiology
subject: FOS: Biological sciences

description: Additional file 1: Text S1. Analyses of soil metabolome. Text S2. Analyses of soil nutrients. Table S1. Basal fertility of the experimental site. Table S2. Year of soybean planting and production. Table S3. Soil nutrients at soybean flowering in 2022. Table S4. Gene catalogue basic information catalogue. Table S5. Table of average relative abundance of species by treatment. SMR: maize–soybean rotation; SC8: 8-year soybean continuous cropping; SC2: 2-year maize, 2-year soybean rotation cropping. Table S6. ANOVA of the top 20 KEGG Level2 functional pathways in relative abundance (%). Results are means ± sd, different letters indicate 0.05 level differences. SMR: maize–soybean rotation; SC8: 8-year soybean continuous cropping; SC2: 2-year maize, 2-year soybean rotation cropping. Table S7. ANOVA of the top 20 KEGG Level3 functional pathways in relative abundance (%). Results are means ± sd, different letters indicate 0.05 level differences. SMR: maize–soybean rotation; SC8: 8-year soybean continuous cropping; SC2: 2-year maize, 2-year soybean rotation cropping. Table S8. ID of the differential metabolites. Table S9. ID of the microbial species level. Figure S1. Monthly average temperature and monthly average rainfall in the experiment site. Figure S2. Relative abundance of microbial taxa stacked histogram at the species level. Figure S3. Box line plot of microbial phyla with relative abundance greater than 1% between treatments. Different letters indicate significant differences at 0.05 level. SMR: maize–soybean rotation; SC8: 8-year soybean continuous cropping; SC2: 2-year maize, 2-year soybean rotation cropping. Figure S4. One-way ANOVA for the top 20 taxa in terms of relative abundance of microorganisms at the genus level (a) and species level (b). Different letters indicate significant differences at 0.05 level. SMR: maize–soybean rotation; SC8: 8-year soybean continuous cropping; SC2: 2-year maize, 2-year soybean rotation cropping. Fig. S5. KEGG pathway PCoA analysis and ANOSIM, Adonis test at the level2 level (a) and level3 level (b). SMR: maize–soybean rotation; SC8: 8-year soybean continuous cropping; SC2: 2-year maize, 2-year soybean rotation cropping. Figure S6. Heatmap of abundance of genes related to P and K metabolism. The Z-values obtained after normalizing the functional relative abundance for each row are presented as the values corresponding to the middle heatmap. SMR: maize–soybean rotation; SC8: 8-year soybean continuous cropping; SC2: 2-year maize, 2-year soybean rotation cropping. Figure S7. Abundance of categories of the significantly different metabolite in SC2 vs SMR (a), SC8 vs SMR (b) and SC2 vs SC8 (c). Heatmap analysis of changes in abundance of soil metabolites that were significantly different between SMR and continuous cropping (SC2, SC8). *Represents 0.05 level differences. SMR: maize–soybean rotation; SC8: 8-year soybean continuous cropping; SC2: 2-year maize, 2-year soybean rotation cropping. Figure S8. Network of Spearman’s correlation between soil nutrients and abundance of N metabolite genes (a) P metabolite genes (b). Figure S9. Heatmap of Spearman’s correlation between yield and abundance of the categories of the significantly different metabolite (a) and different metabolites (b). The different metabolites corresponding to the metabolites numbers in Fig. S4 are detailed in Table S6. *p &lt; 0.05, **p &lt; 0.01, ***p &lt; 0.001. SMR: maize–soybean rotation; SC8: 8-year soybean continuous cropping; SC2: 2-year maize, 2-year soybean rotation cropping.