Additional file 1 of Quartz crystal microbalances (QCM) are suitable for real-time dosimetry in nanotoxicological studies using VITROCELL®Cloud cell exposure systems
Creators: name: Ding, Yaobo
name: Weindl, Patrick
name: Anke-Gabriele Lenz
name: Mayer, Paula
name: Krebs, Tobias
name: Schmid, Otmar
Publisher: name: figshare
Year: 2020
Subjects: subject: Space Science
subject: Medicine
subject: Cell Biology
subject: Molecular Biology
subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: FOS: Chemical sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Developmental Biology
subject: Cancer
subject: 60506 Virology

description: Additional file 1: Table S1. Parameters for calculation of deposition efficiency (Cloud 6). Table S2. Target doses and associated conditions of suspension and nebulization to be adopted during cell exposure. Table S3. Results of F-test on correlations between QCM measurements and fluorescein depositions for both Cloud systems. Table S4. Electronic noise induced precision (relative standard deviation (SD)) and lower detection limit (3 SD) of the VITROCELL® Cloud 6 for 1 Hz QCM data (for 60 s averaged QCM signal) for fluorescein-spiked and pure salt nebulizations. Table S5. Electronic noise induced precision (relative standard deviation (SD)) and lower detection limit (3 SD) of the VITROCELL® Cloud 12 for 1 Hz QCM data (for 30 s averaged QCM signal) for fluorescein-spiked and pure salt. Figure S1. – Photos of the QCM setups in the VITROCELL® Cloud 6 (upper-left) and 12 (upper-right) and corresponding 6-well and 12-well stainless steel inserts for fluorescein deposition (lower-left and lower-right), respectively. Figure S2. TEM pictures of ZnO NM110 (nanoparticle suspension prepared in distilled water before nebulization). Figure S3. Left: Particle volume-size distribution of ZnO nanoparticles dispersed in water revealed volume/mass median diameter and geometric standard deviation of 290 nm of 1.44, respectively (dynamic light scattering, suspension concentration: 1 mg/ml). Right: Comparison of the volume-size distributions before and after nebulization (0.5 mg/ml, liquid droplet was collected in an eppendorf tube upon nebulization and subsequently measured by DLS). Volume/mass- median diameter: 256.7 (before) and 272.7 (after) nm. Figure S4. QCM stability (1 Hz data) at zero-point level (unloaded QCM of Cloud 6) under thermal equilibrium (ca. 37 °C). Three repeated measurements were conducted (T1, T2, T3) for 1 h (3,600 data points each). If the zero point of the QCM is set by the operator just prior to the experiment at an “arbitrarily” selected data point, this can result in a “false” mean zero point level (here between − 53.4 and + 47.3 ng/cm2 (Table S6). Table S6. Numeric evaluation of zero point measurements depicted in Figure S4. Figure S5. Equivalent to Figure S4, but for the Cloud 12 system with a “false” mean zero point level between 3.5 and 21.8 ng/cm2 (Table S7). Table S7. Numeric evaluation of zero point measurements depicted in Figure S5.