Additional file 1 of High-fat diet promotes prostate cancer metastasis via RPS27
Creators: name: Li, Dameng
name: Zhou, Xueying
name: Xu, Wenxian
name: Cai, Yongxin
name: Mu, Chenglong
name: Zhao, Xinchun
name: Tang, Tingting
name: Liang, Chen
name: Yang, Tao
name: Zheng, Junnian
name: Wei, Liang
name: Ma, Bo
Publisher: name: figshare
Year: 2024
Subjects: subject: Biochemistry
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Biological Sciences not elsewhere classified
subject: Mathematical Sciences not elsewhere classified
subject: Cancer
subject: Computational Biology

description: Additional file 1: Supplementary Table 1. Primers for qRT-PCR. Supplementary Table 2. Sequences of shRNAs. Figure S1. Images of liver tissue taken from CD and HFD mice (Related to Fig. 1C&amp;1D). (A) 4 weeks; (B) 5 weeks. Figure S2. Images of HE staining of the liver tissues (Related to Fig. 1G&amp;1H). Figure S3. Protein levels (A) and mRNA expression levels (B) of E-cadherin and vimentin in splenic orthotopic tumors; *P &lt; 0.05; **P &lt; 0.01. Figure S4. Western blot of RPS27 in RWPE1, LNCaP, 22RV1, DU145 and PC3 (Related to Fig. 3A). Figure S5. Images of migration and invasion assay (related to Fig. 3C&amp;3D). (A) DU145 cells with RPS27 overexpression or control; (B) PC3 cells with RPS27 overexpression or control. Figure S6. Images of migration and invasion assay (related to Fig. 3F&amp;3G). (A) DU145 cells with RPS27 knockdown or control; (B) PC3 cells with RPS27 knockdown or control. Figure S7. (A) Images of liver tissues taken from EV and RPS27-OE groups (related to Fig. 4A); (B) HE images of the liver tissues from EV and RPS27-OE groups (related to Fig. 4B&amp;4C). Figure S8. (A) Images of liver tissues taken from NC and RPS27_sh groups (related to Fig. 4D); (B) HE images of the liver tissues from NC and RPS27_sh groups (related to Fig. 4E&amp;F). Figure S9. Images of migration and invasion assay (related to Fig. 3B&amp;3C). (A) Migration; (B) Invasion. Figure S10. (A) Images of liver tissues taken from HFD mice inoculated with control or RPS27_sh DU145 cells (related to Fig. 5D); (B) HE images of the liver tissues from HFD and HFD + SH groups (related to Fig. 5E&amp;5F). Figure S11. (A) Correlation analysis in PRAD tumor between RPS27 and the transcription factors related to NEFA; Analysis was performed by GEPIA2; Correlation coefficient: Pearson; According to the R value, the order is PPRC1, E2F2, PGC1B, NFKB1, NFKB2, PPARD, PPARA, MYC, YAP, FOXO3, PGC1A, E2F1, PPARG; (B) RPS27 was decreased when E2F2 was knockdown in LNCaP cells; Analysis was performed by KnockTF2.0; (C) Kaplan–Meier analysis of the correlation between disease-free survival in prostate adenocarcinoma patients and E2F2 via GEPIA2; Group cutoff: quartile; Datasets selection: PRAD. Figure S12. (A-G) The expression level in EV or RPS27_OE DU145 cells. (A) RPS27; (B) SURF2; (C) FAM83A; (D) AVPI1; (E) TMEM147; (F) TMEM41B; (G) NGRN; (H-N) The expression levels in NC, shRNA_1 and shRNA_2 DU145 cells treated with BSA or NEFA. (H) RPS27; (I) SURF2; (J) FAM83A; (K) AVPI1; (L) TMEM147; (M) TMEM41B; (N) NGRN; Figure S13. Kaplan–Meier analysis of the correlation between disease-free survival in prostate adenocarcinoma patients and SURF2, FAM83A, TMEM41B, TMEM147, NGRN and AVPI1 via GEPIA2; Group cutoff: quartile; Datasets selection: PRAD. Figure S14. IHC images of RPS27 in liver metastatic tumor. (A) Left: Representative images; Right: IHC scores; Images were captured using the Olympus photomicroscope (Tokyo, Japan), and analyzed using ImageJ (IHC Profiler). The staining intensity was scored as 0 (negative), 1 (positive-low), 2 (positive-median), or 3 (positive-strong). The section score was calculated as the percentage contribution of each staining intensity score multiplied by the score; (B) Related to the right panel of (A); ***P &lt; 0.001. Figure S15. Cell growth were detected by CCK8; DU145 or PC3 cells were transfected with RPS27_OE vector or RPS27 siRNAs (si80 + si169), while control group cells were transfected with empty vector or siNC. Figure S16. DU145 cells treated with OA (oleic acid) or PA (palmitic acid); Protein levels of RPS27 and GAPDH were detected by western blot.