cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Purification and properties of Methanobacterium thermoautotrophicum DNA photolyase
Creators: name: HUSAIN, I
name: KIENER, A
name: WALSH, C
name: SANCAR, A
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1989

description: We have purified DNA photolyase from the autotrophic anaerobic archaebacterium Methanobacterium thermoautotrophicum to near homogeneity by a two-column affinity chromatography. The purified enzyme has an Mr = 60,000 and shows near UV absorption peak at 440 nm and a fluorescence emission maximum at 462 nm indicating that it contains 8-hydroxy-5-deazaflavin (coenzyme F420) as an intrinsic chromophore. The photolyase binds with high specificity to thymine dimer in DNA with an equilibrium binding constant, KA = 1.4 x 10(9) M-1, and a dissociation rate constant, koff = 1.4 x 10(-4) s-1 (t1/2 = 43 min). Despite 6-fold higher affinity compared to the folate-containing Escherichia coli photolyase the two enzymes apparently contact the same phosphates around the thymine dimer: the phosphate immediately 5' and the three phosphates immediately 3' to the dimer on the damaged strand and the phosphate across from the dimer in the minor groove on the complementary strand. The absolute action spectrum of the Methanobacterium photolyase in the 400-500-nm region closely matches the absorption of the enzyme-bound F420. The quantum yield (phi) over this region is constant and is approximately 0.2. The value is measurably smaller than the quantum yields reported for other DNA photolyases.
ОПЕРАЦИЯДАН КЕЙИНГИ ВЕНТРАЛ ЧУРРА: ЗАМОНАВИЙ МУАММОЛАР ХОЛАТИ KLINIK HOLATI
Creators: name: Абдурасулов, А.Р.
Publisher: name: Zenodo
Year: 2024

description: Мақолада операциядан кейинги вентрал чурралар (ОКВЧ) частотаси, этиологияси, патогенези ва жарроҳлик тактикаси бўйича адабиётларни кўриб чиқиш берилган. ОКВЧ, эндопротезлар ва классик операцияларнинг замонавий халқаро таснифи тақдим этилган, операциядан кейинги асоратлар ва қониқарсиз натижаларнинг сабаблари тавсифланган.
title: SIMULAÇÃO DE ADMINISTRAÇÃO DE MEDICAMENTOS INTRAVENOSOS: ISIMULA
title: Instrumento de Roteiro de Cenário Simulado
titleType: Subtitle
Creators: name: Roehrs, Hellen
name: Mendes Araújo Silva, Vivianne
name: Eleuterio, Marcelo Campos
Publisher: name: Zenodo
Year: 2024
Subjects: Enfermagem
Treinamento por simulção
Administração de medicação
Matérial didatico
material de ensino
material didático

description: Instrumento de Roteiro de Cenário Simulado para SIMULAÇÃO DE ADMINISTRAÇÃO DE MEDICAMENTOS INTRAVENOSOS: ISIMULA, essa atividade foi aplicada em alunos de graduação em Enfermagem do 4º período. O monitor da disciplina adaptou o material de oficina para o método de simulação realística.
Rat adrenal uptake and metabolism of high density lipoprotein cholesteryl ester
Creators: name: MAHAFFEE, D.
name: GWYNNE, J.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1989

description: Metabolism of high density lipoprotein (HDL) cholesteryl ester (CE) by cultured rat adrenal cells was studied. Addition of [3H]CE-HDL to cells pretreated with adrenocorticotrophin in lipoprotein poor media resulted in a time- and concentration-dependent accumulation of [3H]cholesteryl ester and production of [3H]cholesterol and [3H]corticosterone. HDL-CE metabolism could be described as the sum of a high affinity ([ HDL-cholesterol]1/2 max = 16 micrograms/ml) and low affinity ([ HDL-cholesterol]1/2 max greater than 70 micrograms/ml) process. [3H]Cholesterol was found both intracellularly and in the media. Accumulation of [3H]cholesteryl ester could not be attributed to uptake and re-esterification of unesterified cholesterol since addition of Sandoz 58-035, an inhibitor of acyl coenzyme A:cholesterol acyltransferase, did not prevent ester accumulation. Moreover, addition of chloroquine did not inhibit cholesteryl ester hydrolysis indicating that hydrolysis was not lysosomally mediated. Aminoglutethimide prevented conversion of [3H]CE-HDL to steroid hormones but did not inhibit [3H]cholesteryl ester uptake. Cellular accumulation of [3H] cholesteryl ester exceeded accumulation of 125I-apoproteins 5-fold at 1 h and 35-fold at 24 h indicating selective uptake of cholesteryl ester moiety. We conclude that rat adrenal cells possess a mechanism for selective uptake of HDL cholesteryl esters which provides substrate for steroidogenesis. These results constitute the first direct demonstration that cholesteryl esters in HDL can be used as steroidogenic substrate by the rat adrenal cortex.
Receptor-stimulated phospholipase A2 activation is coupled to influx of external calcium and not to mobilization of intracellular calcium in C62B glioma cells.
Creators: name: Lapetina, EG
name: Mccarthy, Kenny
name: Morell, P
name: Brooks, Robert
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1989

description: C62B rat glioma cells respond to muscarinic cholinergic stimulation with transient inositol phosphate formation and phospholipase A2-dependent arachidonic acid liberation. Since phospholipase A2 is a Ca2+-sensitive enzyme, we have examined the role of the agonist-stimulated Ca2+ response in production of the arachidonate signal. The fluorescent indicator fura-2 was used to monitor changes in cytoplasmic Ca2+ levels ([Ca2+]i) of C62B cells following acetylcholine treatment. In the presence of extracellular Ca2+, acetylcholine induces a biphasic [Ca2+]i response consisting of an initial transient peak that precedes arachidonate liberation and a sustained elevation that outlasts the phospholipase A2 response. The initial [Ca2+]i peak is not altered by the absence of external Ca2+ and therefore reflects intracellular Ca2+ mobilization. The sustained elevation phase is dependent on the influx of external Ca2+; it is lost in Ca2+-free medium and restored on the addition of Ca2+. Pretreating cells with phorbol dibutyrate substantially inhibits acetylcholine-stimulated inositol phosphate formation and the peak [Ca2+]i response without affecting the sustained elevation in [Ca2+]i. This suggests that the release of internal Ca2+ stores by inositol 1,4,5-trisphosphate can be blocked without interfering with Ca2+ influx. Pretreatment with phorbol also fails to affect acetylcholine-stimulated arachidonate liberation, demonstrating that phospholipase A2 activation does not require normal intracellular Ca2+ release. Stimulated arachidonate accumulation is totally inhibited in Ca2+-free medium and restored by the subsequent addition of Ca2+. Pretreatment with verapamil, a voltage-dependent Ca2+ channel inhibitor, also blocks both the sustained [Ca2+]i elevation and arachidonate liberation without altering peak intracellular Ca2+ release. We conclude that the influx of extracellular Ca2+ is tightly coupled to phospholipase A2 activation, whereas large changes in [Ca2+]i due to mobilization of internal Ca2+ stores are neither sufficient nor necessary for acetylcholine-stimulated phospholipase A2 activation.
Additional file 1: of Comparison of microbiota and allergen profile in house dust from homes of allergic and non-allergic subjects- results from the GUSTO study
Creators: name: Loo, Evelyn
name: Lamony Chew
name: Atiqa Zulkifli
name: Ta, Le
name: I-Chun Kuo
name: Goh, Anne
name: Oon Teoh
name: Bever, Hugo Van
name: Gluckman, Peter
name: Yap, Fabian
name: Tan, Kok
name: Yap Chong
name: Lee, Bee
name: Shek, Lynette
Publisher: name: figshare
Year: 2018
Subjects: subject: Space Science
subject: Medicine
subject: Molecular Biology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences

description: Figure S1. Rarefaction curves showing the number of reads per sample against observed operational taxonomic unit (OTU) or Shannon diversity indices of each dust sample. Figure S2. Principal coordinates plots of house dust samples of allergic and non-allergic subjects based on Bray-Curtis dissimilarities. Figure S3. Correlation analysis of the concentrations of tropomyosin and house dust mites of dust samples from Sofa of allergic subjects and non-allergic subjects. (DOCX 1027 kb)
Pheidole psammophila
Creators: name: Wilson, E. O.
Publisher: name: Harvard University Press
Year: 2003
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hymenoptera
Formicidae
Pheidole
Pheidole psammophila

description: Pheidole psammophila Creighton and Gregg
Pheidole psammophila Creighton and Gregg 1955: 15.
Types Mus. Comp. Zool. Harvard.
Etymology Gr psammophila, sand-lover, referring to the nest-site preference (see below).
diagnosis A member of the " bicarinata complex" of the larger pilifera group, comprising agricola, aurea, barbata, bicarinata, centeotl, cerebrosior, defecta, gilvescens, macclendoni, macrops, marcidula, paiute, pinealis, vinelandica, xerophila, yaqui, and yucatana (psammophila is closest to barbata), which complex is characterized by the large to very large, forward-set eyes of both castes; and, in the major, the occipital lobes lacking any sculpturing (except in aurea); the posterior half of the head capsule almost entirely smooth and shiny; and the postpetiolar node seen from above oval, elliptical, or laterally angulate (cornulate in cerebrosior). P. psammophila is distinguished within the complex by the following combination of traits.
Major: hypostoma lacking teeth; humerus in dorsal-oblique view subangulate; petiolar node in side view tapered to a point; small subpostpetiolar process visible in side view; occipital lobes subangulate.
Minor: lower surface of head flat and bearing very long hairs curved to form a basket (psammophore). Petiole long and thin and petiolar and postpetiolar nodes very low.
Measurements (mm) Syntype major: HW 1.60, HL 1.72, SL 0.80, EL 0.30, PW 0.72. Syntype minor: HW 0.54, HL 0.68, SL 0.62, EL 0.24, PW 0.38.
color Major: head and mandibles clear reddish yellow, mesosoma and waist medium reddish brown, gaster and appendages light reddish brown.
Minor: body concolorous medium brown, mandibles a contrasting clear yellow, other appendages light brown.
Range Sandy deserts of southern California, western Arizona, and Sonora, northern Mexico, sea level to 75 m (Creighton and Gregg 1955).
Biology All of the nests found by Creighton and reported in Creighton and Gregg (1955), as well as by Stefan Cover (unpublished notes), were in sand, especially sand subject to frequent shifting, an extreme environment avoided even by other desert-dwelling ants. The remarkable psammophore (basket-like enclosure of hairs between the head, used to carry sand or other loose particles) is likely an adaptation to the environment for which the species is specialized. Chaff piles occurred around the nest entrances of some of the nests, an almost certain sign that psammophila harvests seeds.
Figure Upper: syntype, major. Lower: syntype, minor. CALIFORNIA: 8 km east of Grey's Well, Imperial Co., 50 m (William S. Creighton). Scale bars = 1 mm.
description: Published as part of Wilson, E. O., 2003, Pheidole in the New World. A dominant, hyperdiverse ant genus., Cambridge, MA :Harvard University Press on page 593
Role of enzyme-bound 5, 10-methenyltetrahydropteroylpolyglutamate in catalysis by Escherichia coli DNA photolyase.
Creators: name: Sancar, A
name: Hamm-Alvarez, S
name: Rajagopalan, KV
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1989

description: DNA photolyase catalyzes the photoreversal of pyrimidine dimers. The enzymes from Escherichia coli and yeast contain a flavin chromophore and a folate cofactor, 5,10-methenyltetrahydropteroylpolyglutamate. E. coli DNA photolyase contains about 0.3 mol of folate/mol flavin, whereas the yeast photolyase contains the full complement of folate. E. coli DNA photolyase is reconstituted to a full complement of the folate by addition of 5,10-methenyltetrahydrofolate to cell lysates or purified enzyme samples. The reconstituted enzyme displays a higher photolytic cross section under limiting light. Treatment of photolyase with sodium borohydride or repeated camera flashing results in the disappearance of the absorption band at 384 nm and is correlated with the formation of modified products from the enzyme-bound 5,10-methenyltetrahydrofolate. Photolyase modified in this manner has a decreased photolytic cross section under limiting light. Borohydride reduction results in the formation of 5,10-methylenetetrahydrofolate and 5-methyltetrahydrofolate, both of which are released from the enzyme. Repeated camera flashing results in photodecomposition of the enzyme-bound 5,10-methenyltetrahydrofolate and release of the decomposition products. Finally, it is observed that photolyase binds 10-formyltetrahydrofolate and appears to cyclize it to form the 5,10-methenyltetrahydrofolate chromophore.
Additional file 1: of A discrete event simulation model to evaluate the treatment pathways of patients with cataract in the United Kingdom
Creators: name: Demir, Eren
name: Southern, David
name: Rashid, Syed
name: Lebcir, Reda
Publisher: name: figshare
Year: 2018
Subjects: subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics
subject: Science Policy
subject: Hematology
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: Plant Biology

description: List and values of the parameters in the DES model. The data include the list of the parameters included in the DES model and the numerical values of these parameters. The data represents the situation of the cataract services where the model development was conducted. The data parameters values were entered in the DES model so that it can be run to simulate the current state of cataract services. This simulation run generated the â baselineâ scenario results for the model. (DOCX 14 kb)
Structural and functional characterization of the purified cardiac ryanodine receptor-Ca2+ release channel complex.
Creators: name: Liu, Q Y
name: Rousseau, E
name: Meissner, G
name: Erickson, H P
name: Anderson, K
name: Lai, F A
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1989

description: Using density gradient centrifugation and [3H]ryanodine as a specific marker, the ryanodine receptor-Ca2+ release channel complex from Chaps-solubilized canine cardiac sarcoplasmic reticulum (SR) has been purified in the form of an approximately 30 S complex, comprised of Mr approximately 400,000 polypeptides. Purification resulted in a specific activity of approximately 450 pmol bound ryanodine/mg of protein, a 60-70% recovery of ryanodine binding activity, and retention of the high affinity ryanodine binding site (KD = 3 nM). Negative stain electron microscopy revealed a 4-fold symmetric, four-leaf clover structure, which could fill a box approximately 30 x 30 nm and was thus morphologically similar to the SR-transverse-tubule, junctionally associated foot structure. The structural, sedimentation, and ryanodine binding data strongly suggest there is one high affinity ryanodine binding site/30 S complex, comprised of four Mr approximately 400,000 subunits. Upon reconstitution into planar lipid bilayers, the purified complex exhibited a Ca2+ conductance (70 pS in 50 mM Ca2+) similar to that of the native cardiac Ca2+ release channel (75 pS). The reconstituted complex was also found to conduct Na+ (550 pS in 500 mM Na+) and often to display complex Na+ subconducting states. The purified channel could be activated by micromolar Ca2+ or millimolar ATP, inhibited by millimolar Mg2+ or micromolar ruthenium red, and modified to a long-lived open subconducting state by ryanodine. The sedimentation, subunit composition, morphological, and ryanodine binding characteristics of the purified cardiac ryanodine receptor-Ca2+ release channel complex were similar to those previously described for the purified ryanodine receptor-Ca2+ release channel complex from fast-twitch skeletal muscle.
POMIDOR SHARBATINI TAYYORLASH TEXNOLOGIYASI
Creators: name: Xasanova, Xolida
name: Akbaraliyeva, Fotima
name: Adhamov, Saidvalixon
Publisher: name: Zenodo
Year: 2025

description: Mazkur maqolada pomidor tarkibi, ularning qayta ishlash bosqichlari va sharbat tayyorlash texnologiyasi ilmiy asosda tahlil etilgan. Pomidor inson organizmi uchun muhim bo‘lgan vitamin va minerallar, ayniqsa, likopin moddasi bilan boyitilgan qimmatli mahsulotlardan biri hisoblanadi. Tadqiqot davomida pomidor sharbatini tayyorlash jarayonida amalga oshiriladigan texnologik bosqichlar, jumladan, xom ashyoni saralash, blanshir qilish, maydalash, filtratsiya, pastyerizatsiya va sterilizatsiya jarayonlari atroflicha o‘rganilgan. Shuningdek, mahsulot sifatiga ta’sir etuvchi saqlash sharoitlari va yuzaga keladigan muammolar ilmiy nuqtai nazardan tahlil qilingan.
title: Ethiopian Journal of Environment and Development, Vol. 5 No. 1 (2023): Gondoro as a Social Capital: Analysis of an Indigenous Conflict Resolution and Peacebuilding Practice of the Guji and Gedeo Peoples
title: Gondoro as a Social Capital: Analysis of an Indigenous Conflict Resolution and Peacebuilding Practice of the Guji and Gedeo Peoples
titleType: AlternativeTitle
Creators: name: Ethiopian Journal Of Environment And Development
Publisher: name: Ethiopian Journal of Environment and Development
Year: 2023

description: The people ruled by the Gada system have a tradition of stopping conflict and establishing peace. The Guji and Gede’o people have a tradition of conflict resolution and peacemaking called Gondoro. This research paper aims to show that the Gondoro traditional method of conflict resolution is the social capital of the Guji and Gedeo people. To achieve this purpose, the qualitative descriptive research approach was applied. Through this approach, the Gondoro tradition was described in terms of how it was performed and the traditional practices and their contextual meanings it embodied. A purposive sampling technique was used to select key informants who were cultural group leaders from Guji and Gede’o. In-depth interviews, focus group discussions, and document analysis (archival analysis) were employed for data collection. The data were analyzed by dividing them into different topics to adequately answer the research questions. Based on the analysis of the qualitative data, Gondoro is performed to cleanse ‘fratricide’ that could happen between the Guji and Gedeo people or within the Guji or Gedeo people. It is done to cleanse the grief resulting from the ‘fratricide’ and restore peace among the communities. The article discusses that the Guji and Gede’o people have ‘fraternal’ relationship that has been reinforced through their shared legendary narratives and cultural practices. Accordingly, the article argues that the Gondoro tradition is the social capital of the Guji and Gede’o people. The article concludes that indigenous knowledge, such as the Gondoro tradition, can serve as a good experience for Ethiopian ethnic groups sharing the border to solve their security problems through their common tradition
Pheidole rhea Wheeler
Creators: name: Wilson, E. O.
Publisher: name: Harvard University Press
Year: 2003
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hymenoptera
Formicidae
Pheidole
Pheidole rhea

description: Pheidole rhea Wheeler
Pheidole rhea Wheeler 1908h: 452.
TYPES Unique holotype queen: Department of Entomology Collection, Cornell University, Ithaca, New York.
etymology NL rhea, a South American genus of ostrichlike birds, allusion unknown.
Diagnosis A giant species, reddish to dark brown, tentatively placed in the pilifera group because of the bidentate hypostoma and presence ofa supermajor, but with overall appearance similar to some species of the fallax group. Trimorphic, with major, supermajor, and minor castes, as illustrated. Also distinguished as follows.
Major: head completely covered everywhere except on frontal triangle by dense longitudinal carinulae; promesonotum smoothly convex in both side and dorsal-oblique views; propodeal spines very long; posterior face of propodeum descends vertically; postpetiole diamond-shaped from above.
Supermajor: propodeum descends vertically; postpetiole diamond-shaped from above; cephalic sculpturing as in major, except that the carinulae of the dorsal face behind the level of the eye curve inward toward the cephalic midline. Minor: very long propodeal spines, as illustrated.
Measurements (mm) All castes measured are from Stratton, Santa Catalina Mts., Arizona. Supermajor: HW 3.86, HL 4.04, SL 1.68, EL 0.38, PW 1.38. Major: HW 2.52, HL 2.76, SL 1.40, EL 0.26, PW 1.04. Minor: HW 0.86, HL 0.96, SL 1.06, EL 0.16, PW 0.58.
color Major and minor: body light reddish brown, except for the gaster, which is a slightly contrasting dark reddish brown.
Range Southern Arizona south into Mexico.
Biology The species is found on plateaus and among foothills at the bases of mountains across a wide elevational range, from 1100 to 2100 m, with an apparent preference for the lower end (Creighton 1950a: 168). According to Stefan Cover (personal communication), it "forms enormous colonies surmounted by sloppy craters or under rocks, chiefly on open, rocky slopes. Seeds are harvested in large quantities, but the ants are active predators as well. Mature colonies have well-developed trunk-trail systems. Minors and medias forage, but large majors and supermajors are seldom seen outside the nests. The ants are aggressive, and all size classes actively defend the nest."
figure Upper: major, with partial view of supermajor head at far right. Lower: minor. ARIZONA: Stratton, Santa Catalina Mts., northeast of Tucson. (Type locality: Nogales, Arizona.) Scale bars = 1 mm.
description: Published as part of Wilson, E. O., 2003, Pheidole in the New World. A dominant, hyperdiverse ant genus., Cambridge, MA :Harvard University Press on page 594
title: Ethiopian Journal of Environment and Development, Vol. 5 No. 1 (2023): Effects of Community Based Watershed Management on Rural Livelihoods: The Case of Lume Woreda, East Shewa Zone, Oromia, Ethiopia
title: Effects of Community Based Watershed Management on Rural Livelihoods: The Case of Lume Woreda, East Shewa Zone, Oromia, Ethiopia
titleType: AlternativeTitle
Creators: name: Ethiopian Journal Of Environment And Development
Publisher: name: Ethiopian Journal of Environment and Development
Year: 2023

description: n Ethiopia, natural resource degradation is worsening and posing significant risks, especially to the
livelihoods of rural communities. Community-based watershed management is fundamental for managing natural resource degradation and minimizing its associated risks on the livelihoods of rural communities.
The main objective of this study was to examine the effects of community-based micro watershed management on rural livelihoods. The study used descriptive survey research design to achieve the intended objectives and employed quantitative and qualitative data. The total sample size of 251 respondents was taken using a systematic random sampling technique from a study population of 2595 households inhabited in four selected Kebeles. A questionnaire, interviews, field observation, and document analysis were used to collect the data. Besides, statistical methods such as percentage of frequencies, mean, standard deviation, bar graphs, paired sample t-test, and chi-square test were used in data analysis.
Spectral Anomaly and High Temperature Superconductors
Creators: name: Yin, L.
name: Chakravarty, S.
Publisher: name: arXiv
Year: 1995
Subjects: subject: Condensed Matter (cond-mat)
subject: FOS: Physical sciences

description: Spectral anomaly for interacting Fermions is characterized by the spectral function $A([k-k_F],ω)$ satisfying the scaling relation $A(Λ^{y_1} [k-k_F],Λ^{y_2}ω)= Λ^{y_A}A([k-k_F],ω)$, where $y_1$, $y_2$, and $y_A$ are the exponents defining the universality class. For a Fermi liquid $y_1=1$, $y_2=1$, $y_A=-1$; all other values of the exponents are termed anomalous. In this paper, an example for which $y_1=1$, $y_2=1$, but $y_A=α-1$ is considered in detail. Attractive interaction added to such a critical system leads to a novel superconducting state, which is explored and its relevance to high temperature cuprate superconductors is discussed.
description: RevTex, 53 pages (including figures)
Structural and functional correlation of the trypsin-digested Ca2+ release channel of skeletal muscle sarcoplasmic reticulum.
Creators: name: Meissner, G
name: Lai, FA
name: Rousseau, E
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1989

description: The effect of trypsin digestion on the (i) fragmentation pattern, (ii) activity, (iii) [3H]ryanodine binding, and (iv) sedimentation behavior of the skeletal sarcoplasmic reticulum (SR) ryanodine receptor-Ca2+ release channel complex has been examined. Mild tryptic digestion of heavy, junctional-derived SR vesicles resulted in the rapid disappearance of the high molecular weight (Mr approximately 400,000) Ca2+ release channel protein on sodium dodecyl sulfate gels and appearance of bands of lower Mr upon immunoblot analysis, without an appreciable effect on [3H]ryanodine binding or the apparent S value (30 S) of the 3-[3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (Chaps)-solubilized channel complex. Further degradation to bands of Mr greater than 70,000 on immunoblots correlated with a reduction of channel size from 30 S to 10-15 S and loss of high affinity [3H]ryanodine binding to the trypsinized receptor, while low affinity [3H]ryanodine binding and [3H]ryanodine bound prior to digestion were retained. Parallel 45Ca2+ efflux measurements also indicated retention of the Ca2+, Mg2+, and ATP regulatory sites, although Ca2+-induced 45Ca2+ release rates were changed. In planar lipid bilayer-single channel measurements, addition of trypsin to the cytoplasmic side of the high conductance (100 pS in 50 mM Ca2+), Ca2+-activated SR Ca2+ channel initially increased the fraction of channel open time and was followed by a complete and irreversible loss of channel activity. Trypsin did not change the unitary conductance, and was without effect on single channel activity when added to the lumenal side of the channel.
Linear instability of Poiseuille flows with highly non-ideal fluids
Creators: name: Ren, Jie
name: Fu, Song
name: Pecnik, Rene
Publisher: name: arXiv
Year: 2018
Subjects: subject: Fluid Dynamics (physics.flu-dyn)
subject: FOS: Physical sciences

description: The objective of this work is to investigate linear modal and algebraic instability in Poiseuille flows with fluids close to their vapour-liquid critical point. Close to this critical point, the ideal gas assumption does not hold and large non-ideal fluid behaviours occur. As a representative non-ideal fluid, we consider supercritical carbon dioxide (CO$_2$) at pressure of 80 bar, which is above its critical pressure of 73.9 bar. The Poiseuille flow is characterized by the Reynolds number ($Re=ρ_{w}^{*}u_{r}^{*}h^{*}/μ_{w}^{*}$), the product of Prandtl ($Pr=μ_{w}^{*}C_{pw}^{*}/κ_{w}^{*}$) and Eckert number ($Ec=u_{r}^{*2}/C_{pw}^{*}T_{w}^{*}$), and the wall temperature that in addition to pressure determines the thermodynamic reference condition. For low Eckert numbers, the flow is essentially isothermal and no difference with the well-known stability behaviour of incompressible flows is observed. However, if the Eckert number increases, the viscous heating causes gradients of thermodynamic and transport properties, and non-ideal gas effects become significant. Three regimes of the laminar base flow can be considered, subcritical (temperature in the channel is entirely below its pseudo-critical value), transcritical, and supercritical temperature regime. If compared to the linear stability of an ideal gas Poiseuille flow, we show that the base flow is more unstable in the subcritical regime, inviscid unstable in the transcritical regime, while significantly more stable in the supercritical regime. Following the corresponding states principle, we expect that qualitatively similar results will be obtained for other fluids at equivalent thermodynamic states.
description: 34 pages, 22 figures
Structural and functional properties of human alpha-thrombin, phosphopyridoxylated alpha-thrombin, and gamma T-thrombin. Identification of lysyl residues in alpha-thrombin that are critical for heparin and fibrin (ogen) interactions.
Creators: name: Sherrill, GB
name: Kalayanamit, T
name: Church, FC
name: Tobin, RB
name: Pratt, CW
name: Noyes, CM
name: Meade, JB
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1989

description: alpha-Thrombin derivatives obtained either by site-specific modification at lysyl residues (phosphopyridoxylated) or by limited trypsinolysis (gamma T-thrombin) were compared to correlate structural modifications with the functional reactivity toward fibrin(ogen) and heparin. alpha-Thrombin phosphopyridoxylated in the absence of heparin (unprotected) showed approximately 2 mol of label incorporated/mol of thrombin, but only 1 mol of label incorporated/mol of proteinase when modified in the presence of added heparin (protected). In contrast to native alpha-thrombin, both phosphopyridoxylated alpha-thrombin derivatives failed to interact with a fibrin monomer-agarose column and had reduced fibrinogen clotting activity, which is very similar to gamma T-thrombin. Heparin accelerated the rate of antithrombin III inhibition of alpha-thrombin, heparin-protected modified-alpha-thrombin, and gamma T-thrombin in a manner consistent with a template mechanism but was without effect on unprotected modified alpha-thrombin. In a heparin-catalyzed antithrombin III inhibition assay of alpha-thrombin, we found that D-Phe-Pro-Arg chloromethyl ketone-active site-inactivated gamma T-thrombin competed for heparin binding. It has been shown that limited proteolysis/autolysis of the B-chain of alpha-thrombin in the area around Arg-B73 (in beta T/beta- and gamma T/gamma-thrombin), but not that around Lys-B154 (in gamma T/gamma-thrombin), diminishes specific interactions with fibrinogen (Hofsteenge, J., Braun, P. J., and Stone , S. R. (1988) Biochemistry 27, 2144-2151). In unprotected modified alpha-thrombin, lysyl residues B21, B65, B174, and B252 were phosphopyridoxylated. In heparin-protected modified alpha-thrombin, only lysyl residues B21 and B65 were phosphopyridoxylated. These observations suggest that lysyl residues 21/65 of the B-chain of alpha-thrombin are involved in fibrin(ogen) interactions, and lysyl residues 174/252 of the B-chain are important in heparin interactions.
Studies on the active site of the Neurospora crassa plasma membrane H+-ATPase with periodate-oxidized nucleotides
Creators: name: Scarborough, G.A.
name: Morjana, N.A.
name: Bidwai, A.P.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1989

description: The Neurospora crassa plasma membrane H+-ATPase is inactivated by the periodate-oxidized nucleotides, oATP, oADP, and oAMP, with oAMP the most effective. Inhibition of the ATPase is essentially irreversible, because Sephadex G-50 column chromatography of the oAMP-treated ATPase does not result in a reversal of the inhibition. Inhibition of the ATPase by oAMP is protected against by the H+-ATPase substrate ATP, the product ADP, and the competitive inhibitors TNP (2',3'-O-(2,4,6-trinitrocyclohexadienylidine)-ATP and TNP-ADP, suggesting that oAMP inhibition occurs at the nucleotide binding site of the enzyme. The rate of inactivation of the ATPase by oAMP is only slightly affected by EDTA, indicating that the oAMP interaction with the nucleotide binding site of the H+-ATPase occurs in the absence of a divalent cation. The protection against oAMP inhibition by ADP is likewise unaffected by EDTA. The inhibition of the ATPase by oAMP is absolutely dependent on the presence of acidic phospholipids or acidic lysophospholipids known to be required for H+-ATPase activity, suggesting that these lipids either aid in the formation of the nucleotide binding site or render it accessible. Incubation of the ATPase with Mg2+ plus vanadate, which locks the enzyme in a conformation resembling the transition state of the enzyme dephosphorylation reaction, completely protects against inhibition by oAMP, suggesting that in this transition state conformation the nucleotide site either does not exist, or is inaccessible to oAMP. Labeling studies with [14C] oAMP indicate that the incorporation of 1 mol of oAMP is sufficient to cause complete inactivation of the ATPase.
QED effective action at finite temperature
Creators: name: Gies, Holger
Publisher: name: arXiv
Year: 1998
Subjects: subject: High Energy Physics - Phenomenology (hep-ph)
subject: FOS: Physical sciences

description: The QED effective Lagrangian in the presence of an arbitrary constant electromagnetic background field at finite temperature is derived in the imaginary-time formalism to one-loop order. The boundary conditions in imaginary time reduce the set of gauge transformations of the background field, which allows for a further gauge invariant and puts restrictions on the choice of gauge. The additional invariant enters the effective action by a topological mechanism and can be identified with a chemical potential; it is furthermore related to Debye screening. In concordance with the real-time formalism, we do not find a thermal correction to Schwinger's pair-production formula. The calculation is performed on a maximally Lorentz covariant and gauge invariant stage.
description: 9 pages, REVTeX, 1 figure, typos corrected, references added, final version to appear in Phys. Rev. D
Quasar host galaxies in GAMA
Creators: name: Kotilainen, Jari
name: Acharya, Nischal
name: De Propris, Roberto
name: Wethers, Clare
Publisher: name: Zenodo
Year: 2019

description: We present first results from our study of the host galaxies and environments of quasars in Galaxy And Mass Assembly (GAMA), a multiwavelength photometric and spectroscopic survey for ~300 000 galaxies over ~300 deg2, to a limiting magnitude of r ~20 mag. We use a GAIA-selected sample of ~220 quasars at z < 0.3 in GAMA. For all the quasars, we determine all surrounding GAMA galaxies and measure their star formation (SF) rate and SF history, and the host galaxy morphology and group membership of the quasars. As a comparison sample of inactive galaxies, we use 1000 subsets of galaxies in GAMA, matched in redshift and galaxy stellar mass to the quasars. We find that quasar activity does not depend on the large-scale environment (cluster/group/void), although quasars tend to prefer satellite location in their environment. Compared to inactive galaxies, quasars are preferentially hosted in bulge-dominated galaxies and have higher SF rates, both overall and averaged over the last 10 and 100 Myr. Quasars also have shorter median SF timescales, shorter median time since the last SF burst, and higher metallicity than inactive galaxies. We discuss these results in terms of triggering mechanisms of the quasar activity and the role of quasars in galaxy evolution.
MOESM1 of Growth, inequality and poverty dynamics in Mexico
Creators: name: Iniguez-Montiel, Alberto
name: Kurosaki, Takashi
Publisher: name: figshare
Year: 2018
Subjects: subject: Ecology
subject: FOS: Biological sciences
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Computational Biology

description: Additional file 1. Appendix.
Photonic single sideband RF generator based on an integrated optical micro-ring resonator
Creators: name: Xu, Xingyuan
name: Wu, Jiayang
name: Tan, Mengxi
name: Nguyen, Thach G.
name: Chu, Sai T.
name: Little, Brent E.
name: Morandotti, Roberto
name: Mitchell, Arnan
name: Moss, David J.
Publisher: name: arXiv
Year: 2018
Subjects: subject: Signal Processing (eess.SP)
subject: FOS: Electrical engineering, electronic engineering, information engineering

description: We demonstrate narrowband orthogonally polarized optical RF single sideband generation as well as dual-channel equalization based on an integrated dual-polarization-mode high-Q microring resonator. The device operates in the optical communications band and enables narrowband RF operation at either 16.6 GHz or 32.2 GHz, determined by the free spectral range and TE/TM mode interval in the resonator. We achieve a very large dynamic tuning range of over 55 dB for both the optical carrier-to-sideband ratio and the dual-channel RF equalization.
description: 10 pages, 13 Figures, 53 references
Pheidole rugulosa Gregg
Creators: name: Wilson, E. O.
Publisher: name: Harvard University Press
Year: 2003
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hymenoptera
Formicidae
Pheidole
Pheidole rugulosa

description: Pheidole rugulosa Gregg
Pheidole rugulosa Gregg 1959: 26.
Types Mus. Comp. Zool. Harvard.
Etymology L rugulosa, a small amount of wrinkling, evidently referring to the occipital rugulation.
Diagnosis A member of the " pilifera complex" of the larger pilifera group; for a characterization of the complex, see under pilifera.
P. rugulosa is distinguished within the complex by the following combination of traits.
Major: rugulae on occiput seen in full-face view limited to a narrow strip; longitudinal carinulae originating on the frontal triangle travel all the way to meet the occipital rugulae, but other cephalic carinulae do not reach beyond the midpoint of the head capsule; anterior strip of the pronotum transversely carinulate; mesonotal convexity very low; postpetiole seen from above laterally angulate. Minor: exceptionally large, forward-placed eyes; mesonotal convexity present in dorsal-oblique view; propodeal spines well developed.
Throughout much of southern New Mexico and Arizona, P. rugulosa occurs with P. soritis, with which it is easily confused.
P. rugulosa is most easily distinguished from that species by the major's well-developed humeral angles, which are inconspicuous and rounded in soritis, and the large shiny areas on the posterior dorsal surface of the head, which is variably sculptured in soritis.
Measurements (mm) Paratype major: HW 1.04, HL 1.12, SL 0.54, EL 0.22, PW 0.56.
Paratype minor: HW 0.52, HL 0.52, SL 0.42, EL 0.14, PW 0.44.
Color Major: reddish to medium brown, gaster often somewhat darker. Minor: medium to dark brown, even blackish.
Range Texas, New Mexico, Arizona.
biology Stefan Cover (unpublished collection notes) found numerous colonies of rugulosa in the deserts of southern Arizona and southwestern New Mexico at 1300-1700 m, in xeric habitats ranging from grazed grassland desert to rabbitbrush-mesquite desert, mesquite-acacia dominated wash, and riverbank desert scrub. The ants usually nest in the open soil, with the entrances surrounded by either crescentic or full craters; the entrance of one nest was in a grass clump. Similar nest site preferences are reported from western Texas by Moody and Francke (1982). According to Cover, colonies may consist of several hundred individuals or more, and, unusually for Pheidole, are at least sometimes polygynous. Seeds are occasionally found in the nests.
figure Upper: paratype, major. Lower: paratype, minor. ARIZONA: Tucson. Scale bars = 1 mm.
description: Published as part of Wilson, E. O., 2003, Pheidole in the New World. A dominant, hyperdiverse ant genus., Cambridge, MA :Harvard University Press on page 595
Synthetic amphipathic peptides resembling apolipoproteins stimulate the release of human placental lactogen
Creators: name: Handwerger, S
name: Anantharamaiah, G
name: Segrest, J
name: Anantharamaiah, Gm
name: Jorgensen, E
name: Segrest, Jere
name: Gwynne, J
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1989

description: Previous studies from our laboratory demonstrated native high density lipoproteins and apolipoproteins AI, AII, and CI, stimulate the release of human placental lactogen (hPL) from trophoblast cells in culture. To examine the mechanisms by which these apolipoproteins stimulate hPL release, we have studied hPL secretion in response to several synthetic peptide analogs of the amphipathic helical structure of the apolipoproteins. The magnitude of the stimulation of hPL release in response to the analog peptides correlated with the ability to displace apolipoproteins from high density lipoprotein and with other measures of phospholipid binding affinity such as the increase in alpha-helicity and the size of complexes formed between the peptide and phospholipid. The correlation of stimulatory ability and lipid affinity suggests that the action of the apolipoproteins on hPL release may be mediated through an interaction with plasma membrane phospholipids.
title: Ethiopian Journal of Environment and Development, Vol. 5 No. 1 (2023): Plant-pollinator interactions of three selected plant species in Gullele Botanic Garden, Addis Ababa, Ethiopia
title: Plant-pollinator interactions of three selected plant species in Gullele Botanic Garden, Addis Ababa, Ethiopia
titleType: AlternativeTitle
Creators: name: Ethiopian Journal Of Environment And Development
Publisher: name: Ethiopian Journal of Environment and Development
Year: 2023

description: Assessment of plant-pollinator interaction in three selected naturally grown plant species (Rosa abyssinica,Hypericum revolutum, and Vernonia leopoldi) was conducted using pollination observation method in the Gullele Botanic Garden, Addis Ababa, Ethiopia. Critical observation of pollinators was conducted while visiting the floral parts of each species, capturing a photo of each pollinator on the floral part, and at the same time recording the time and giving the general name of pollinators. This observation activity was conducted by walking along the garden, choosing any flowering individual for about 30 min observations for five flowers. Data was collected in five weeks between November and December 2021 about the plant-pollinator
visits, pollinators’ diversity, number of visits to each species, and pollinators’ preferred time in a day were
analyzed using descriptive analysis. A total of six functional groups (bees-Hymnoptera, beetles-Coleoptera,
flies-Diptera, moths and butterflies-Lepidoptera, and bird) were recorded from pollination observation. Bees were the most flower-visiting/pollinating insect group for the three species. Beetles were the second most visited insects on the flowers of the three species, followed by flies, moths, butterflies, and birds. Plants attracted a range of insects, with bees as the most abundant visitor/pollinator, accounting for 88% of the total visits. The rate of insect flower visits for the three species indicated a decrease from the first to the fifth week of floral blooming. For mutual benefit and sustainable conservation of selected flowering plants and pollinating insects, it would be important to promote the botanic garden by establishing a pollinator garden as part of the thematic garden.
title: Inscribed Mesopotamian Artefacts in the Brighton Museum and Art Gallery, UK
Creators: name: Frazer, Mary
Publisher: name: Editorial Ausa
Year: 2017

description: This article publishes ten of the eleven inscribed Mesopotamian artefacts in the Brighton Museum and Art Gallery. It gives special attention to an Ur III tablet that contains half of an account concerning deliveries by various herdsmen in Umma province over a six-year period.
Additional file 1: of Methods for evaluating adverse drug event preventability in emergency department patients
Creators: name: Woo, Stephanie
name: Cragg, Amber
name: Wickham, Maeve
name: Peddie, David
name: Balka, Ellen
name: Scheuermeyer, Frank
name: Villanyi, Diane
name: Hohl, Corinne
Publisher: name: figshare
Year: 2018
Subjects: subject: Medicine
subject: Pharmacology
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Science Policy
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Appendix A. Data Collection Form for Preventability Assessments. (DOCX 18 kb)
title: Ethiopian Journal of Environment and Development, Vol. 5 No. 1 (2023): Unveiling the shift of smallholders’ Cash Crop Farming from Coffee-orchard to Khat in Sidama region, Ethiopia
title: Unveiling the shift of smallholders’ Cash Crop Farming from Coffee-orchard to Khat in Sidama region, Ethiopia
titleType: AlternativeTitle
Creators: name: Ethiopian Journal Of Environment And Development
Publisher: name: Ethiopian Journal of Environment and Development
Year: 2023

description: The study unveiled the recent competing shifting of cash cropping smallholders’ farmland use from coffee and orchard fruits to khat farming based on the empirical evidence collected from the Sidama region, Ethiopia. Despite the national economic and sociocultural importance of coffee in Ethiopia, large tracts of coffee farmland are being cleared and converted to khat cropping in Sidama, Ethiopia. A mixed research design with both quantitative and qualitative approaches was used. The sampling technique was multistage. The multistage sampling technique that combined purposive, stratification and simple random sampling techniques was used. Two woredas, namely Dara and Aleta Chuko were purposely selected, and from Dara, 190 respondents were randomly selected from three kebeles, and from Aleta Chuko, 192 respondents were selected randomly from three kebeles, and a total of 382 farm households were selected from the study area.
Primary data was collected using survey questionnaires, interviews, observation, and focus group discussions. The logistic regression technique was used to identify the determinants of farmland shift from coffee and orchard fruit farming to khat farming. The study revealed that in one district of the study area, khat plantation
coverage increased from 86 hectares in 2018/19 to 170 hectares in 2019/20. In this district, from twenty-four kebeles (the smallest administrative unit), sixteen kebeles were coffee producers. Currently, three of them are converted by khat cropping. Among other factors, market bureaucracy, market access, and income strongly contributed to the shift of coffee farmland into khat farming. Furthermore, the study revealed that the shift is poverty-induced and is an alternative to the ever-challenging smallholder’s livelihood, and the expansion of khat farming brought negative sociocultural and political consequences to the local community. On the contrary, the government follows non-involvement farmland use policy to khat production and marketing for undefined reasons. We confirmed some actors, such as local state authorities, brokers, and traders have networked interests in khat production and marketing. The study contributes to policy debate, dialogue, and inputs on cash crop farming.