title: ÐÑÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð¸ Ñ Ð°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ° ÑÐºÐ·Ð¾ÑÐ¾Ð¼, ÑÐµÐºÑÐµÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ Ð¸Ð½ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¼Ð¸ Ð²Ð¸ÑÑÑÐ¾Ð¼ Ð³ÑÐ¸Ð¿Ð¿Ð° Ð ÐºÐ»ÐµÑÐºÐ°Ð¼Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Isolation and characterization of exosomes secreted by influenza A virus-infected cells
Creators: name: ÐÐ°Ð²ÑÐ¸Ð»Ð¾Ð²Ð°, ÐÐ¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÐÐ¸ÑÑÑÐ¾Ð»Ð¾Ð³Ð¸Ñ
ÑÐºÐ·Ð¾ÑÐ¾Ð¼Ñ
Ð²Ð¸ÑÑÑ Ð³ÑÐ¸Ð¿Ð¿Ð°
Ð¼ÐµÑÐ¾Ð´Ð¸ÐºÐ° ÑÐ°Ð·Ð´ÐµÐ»ÐµÐ½Ð¸Ñ ÑÐºÐ·Ð¾ÑÐ¾Ð¼ Ð¸ Ð²Ð¸ÑÑÑÐ° Ð³ÑÐ¸Ð¿Ð¿Ð°
Ñ ÑÐ¾Ð¼Ð°ÑÐ¾Ð³ÑÐ°ÑÐ¸Ñ
Ð³ÑÐ°Ð´Ð¸ÐµÐ½Ñ ÑÐ°Ñ Ð°ÑÐ¾Ð·Ñ
exosomes
influenza virus
method for separating of exosomes and influenza virus
chromatography
sucrose gradient

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð²ÑÐ±Ð¾ÑÑ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ ÑÑÐ¿ÐµÑÐ½Ð¾Ð³Ð¾ Ð¼ÐµÑÐ¾Ð´Ð° Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ ÑÐ¸ÑÑÑÑ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð² Ð¸ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐµ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² ÑÐ°Ð·Ð´ÐµÐ»ÐµÐ½Ð¸Ñ ÑÐºÐ·Ð¾ÑÐ¾Ð¼ Ð¸ ÑÐ°ÑÑÐ¸Ñ Ð²Ð¸ÑÑÑÐ° Ð³ÑÐ¸Ð¿Ð¿Ð° Ð. ÐÐ»Ñ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ ÑÑÐ¿ÐµÑÐ½Ð¾Ð³Ð¾ Ð¼ÐµÑÐ¾Ð´Ð° Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ ÑÐºÐ·Ð¾ÑÐ¾Ð¼, ÑÐ°ÑÑÐ¼Ð°ÑÑÐ¸Ð²Ð°Ð»Ð¸ ÑÑÐ¸ Ð¼ÐµÑÐ¾Ð´Ð°: ÑÐ»ÑÑÑÐ°ÑÐµÐ½ÑÑÐ¸ÑÑÐ³Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ, Ð³ÐµÐ»Ñ-ÑÐ¸Ð»ÑÑÑÐ°ÑÐ¸Ñ Ð¸ ÐºÐ¾Ð¼Ð¼ÐµÑÑÐµÑÐºÐ¸Ð¹ Ð½Ð°Ð±Ð¾Ñ Ð´Ð»Ñ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Â«Total exosome isolation reagentÂ». ÐÐ»Ñ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð² Ð¿Ð¾ÑÐ»Ðµ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¿ÑÐ¸Ð¼ÐµÐ½ÑÐ»Ð¸ Ð¼ÐµÑÐ¾Ð´Ñ Ð´Ð¾Ñ-Ð±Ð»Ð¾ÑÑÐ¸Ð½Ð³Ð°, ÐÐ­Ð Ð¸ ÐÐ Ð¡. Ð¡Ð¾Ð³Ð»Ð°ÑÐ½Ð¾ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð², Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð»ÑÑÑÐµÐ³Ð¾ Ð¼ÐµÑÐ¾Ð´Ð° Ð±ÑÐ» Ð²ÑÐ±ÑÐ°Ð½ Ð¼ÐµÑÐ¾Ð´Ð° ÑÐ»ÑÑÑÐ°ÑÐµÐ½ÑÑÐ¸ÑÑÐ½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ, ÑÐ°Ðº ÐºÐ°Ðº Ð¿ÑÐ¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ð¸ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð² ÑÑÐ¸Ð¼ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð¿Ð¾ÑÐµÑÑ ÑÐºÐ·Ð¾ÑÐ¾Ð¼ Ð±ÑÐ»Ð° Ð¼Ð¸Ð½Ð¸Ð¼Ð°Ð»ÑÐ½Ð°Ñ.ÐÑÐ¾ÑÐ¾Ð¹ Ð·Ð°Ð´Ð°ÑÐµÐ¹ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð° ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð¼ÐµÑÐ¾Ð´Ð¾Ð² ÑÐ°Ð·Ð´ÐµÐ»ÐµÐ½Ð¸Ñ ÑÐºÐ·Ð¾ÑÐ¾Ð¼ Ð¸ ÑÐ°ÑÑÐ¸Ñ Ð²Ð¸ÑÑÑÐ° Ð³ÑÐ¸Ð¿Ð¿Ð°. Ð ÑÐ¾Ð´Ðµ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ Ð±ÑÐ»Ð¾ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð¾ Ð´Ð²Ð° Ð¼ÐµÑÐ¾Ð´Ð°: ÑÑÐ¾Ð¼Ð°ÑÐ¾Ð³ÑÐ°ÑÐ¸ÑÐµÑÐºÐ¾Ðµ ÑÐ°Ð·Ð´ÐµÐ»ÐµÐ½Ð¸Ðµ, Ð¿ÑÐ¸ Ð¿Ð¾Ð¼Ð¾ÑÐ¸ ÑÐ¾ÑÐ±ÐµÐ½ÑÐ° ÑÐµÑÐ°ÐºÑÐ¸Ð» Ð¿Ð¾ Ð¿ÑÐ¸Ð½ÑÐ¸Ð¿Ñ Ð³Ð¸Ð´ÑÐ¾ÑÐ¾Ð±Ð½Ð¾Ð³Ð¾ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ, Ð¸ ÑÐ°Ð·Ð´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð½Ð° Ð³ÑÐ°Ð´Ð¸ÐµÐ½ÑÐµ ÑÐ°ÑÐ°ÑÐ¾Ð·Ñ. ÐÐ»Ñ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð², Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð¿Ð¾ÑÐ»Ðµ ÑÐ°Ð·Ð´ÐµÐ»ÐµÐ½Ð¸Ñ, Ð¿ÑÐ¸Ð¼ÐµÐ½ÑÐ»Ð¸ Ð¼ÐµÑÐ¾Ð´Ñ Ð´Ð¾Ñ-Ð±Ð»Ð¾ÑÑÐ¸Ð½Ð³Ð° Ð¸ ÐÐ­Ð. ÐÐ±Ð° ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð½ÑÑ Ð¼ÐµÑÐ¾Ð´Ð° Ð¿Ð¾Ð»ÑÑÐ¸Ð»Ð¸ÑÑ ÑÑÐ¿ÐµÑÐ½ÑÐ¼Ð¸ Ð¸ Ð³Ð¾ÑÐ¾Ð²ÑÐ¼Ð¸ Ðº Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ. ÐÐ°Ð¶Ð½Ð¾ Ð¾ÑÐ¼ÐµÑÐ¸ÑÑ, ÑÑÐ¾ Ð¼ÐµÑÐ¾Ð´ ÑÐ°Ð·Ð´ÐµÐ»ÐµÐ½Ð¸Ñ ÑÐºÐ·Ð¾ÑÐ¾Ð¼ Ð¸ Ð²Ð¸ÑÑÑÐ½ÑÑ ÑÐ°ÑÑÐ¸Ñ Ð½Ð° Ð³ÑÐ°Ð´Ð¸ÐµÐ½ÑÐµ ÑÐ°ÑÐ°ÑÐ¾Ð·Ñ Ð¼Ð¾Ð¶ÐµÑ Ð±ÑÑÑ Ð¼Ð°ÑÑÑÐ°Ð±Ð¸ÑÐ¾Ð²Ð°Ð½ Ð² Ð±ÑÐ´ÑÑÐµÐ¼ Ð½Ð° Ð¿ÑÐµÐ´Ð¿ÑÐ¸ÑÑÐ¸Ñ, ÐºÐ¾ÑÐ¾ÑÑÐµ Ð·Ð°Ð½Ð¸Ð¼Ð°ÑÑÑÑ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ¾Ð¹ Ð²Ð°ÐºÑÐ¸Ð½ Ð¸Ð»Ð¸ Ð»ÐµÐºÐ°ÑÑÑÐ²ÐµÐ½Ð½ÑÑ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð², Ð¿ÑÐ¸ Ð¿Ð¾Ð¼Ð¾ÑÐ¸ ÑÐºÐ·Ð¾ÑÐ¾Ð¼.
description: This work is devoted to the choice of the most successful method for the isolation of pure preparations and the development of methods for the separation of exosomes and influenza A virus particles. To determine the most successful exosome isolation method, three methods were considered: ultracentrifugation, gel filtration, and the commercial Total exosome isolation reagent kit. Dot blotting, TEM, and DLS were used to analyze the obtained preparations after isolation. According to the results obtained, the ultracentrifugation method was chosen as the best method, since the loss of exosomes was minimal when obtaining preparations by this method. The second objective of this work was to develop methods for separating exosomes and influenza virus particles. In the course of the research, two methods were developed: chromatographic separation using the Sephacryl sorbent according to the principle of hydrophobic interaction, and separation on a sucrose gradient. Dot blot and TEM methods were used to analyze preparations obtained after separation. Both developed methods turned out to be successful and ready for use. It is important to note that the sucrose gradient method of separating exosomes and viral particles can be scaled up in the future to enterprises that develop vaccines or drugs using exosomes.