cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Design of diblock co-oligomers as low bandgap small molecules for organic solar cells
Creators: name: Oliveira, Eliezer Fernando
name: Lavarda, Francisco Carlos
Publisher: name: Taylor & Francis
Year: 2017
Subjects: subject: Biophysics
subject: Biochemistry
subject: 29999 Physical Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: Molecular Biology
subject: Neuroscience
subject: Physiology
subject: FOS: Biological sciences
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences

description: The properties of a particular kind of small molecule that is built from two oligomers of different monomers, i.e. a diblock co-oligomer, as the electron donor in the active layer of organic solar cells are investigated theoretically. For these molecules, this work shows that it is possible to predict the energies of the frontier molecular orbitals by knowing the same energies for the oligomers that constitute the diblock, opening the possibility of designing new materials with optimal energy levels and optical properties. Furthermore, it was observed that the optical absorption bands of these diblock co-oligomers were broader than that of the constituent oligomers and also of the homopolymers, allowing greater absorption of photons and possibly an improved electric current in the device. It was also shown that these phenomena are size-dependent.
In Other Words: Writing Maurice Blanchot Writing
Creators: name: Duffy, Nikolai
Publisher: name: Monash University
Year: 2017
Subjects: subject: 200599 Literary Studies not elsewhere classified
subject: FOS: Languages and literature

description: There is something undeniably curious about the work of Maurice Blanchot. His writings would seem to belong to the obscurities of the night in such a way that they refuse critical interpretation even as they suggest the possibility of a reflection on the practice of literary-critical reading. There is a sense in which Blanchot is to be located always someplace else, both somewhere beyond his text and sometime not yet contemporaneous with it. The imprint of his hand might hover over the grain of the page cast-ing its hermitic shadow and guarding against what might gather itself there, but this hand, if ever it did, no longer belongs to him. The writer writes, Blanchot argues, to the extent that the writer has always already been writ-ten by writing; writing is what the writer must give up in order to write so that writing, in fact, would carry always within itself a resistance to any of the advances a hermeneutic or metalanguage might feign to hazard.
Recruitment of cyanobacteria from the sediments in the eutrophic Shanzi Reservoir
Creators: name: Su, Yuping
name: You, Xuejing
name: Lin, Hui
name: Zhuang, Huiru
name: Weng, Yuan
name: Zhang, Dayi
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Environmental Sciences not elsewhere classified
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: Biological Sciences not elsewhere classified
subject: Inorganic Chemistry
subject: FOS: Chemical sciences
subject: Science Policy

description: This study investigated the impact of four environmental factors on the recruitment of cyanobacteria from bottom sediments in the eutrophic Shanzi Reservoir. Temperature and light were identified as the key determinants for the recruitment of Microcystis and Oscillatoria. Cyanobacteria became dominant at higher temperature (20°C) and light intensity (2000 lx) and Microcystis and Oscillatoria were the major species. Detailed recruitment simulation undertaken with the respective gradients of temperature and light suggested that both Microcystis and Oscillatoria are temperature sensitive and that their critical temperature point was 10°C. However, distinct light impacts were observed only on Microcystis. The recruitment of Oscillatoria was light independent, whereas Microcystis had a positive relationship with light intensity. Physical disturbance promoted Microcystis recruitment and also affected the structure of the recruited cyanobacterial community at the water–sediment interface, based on quantitative polymerase chain reaction (qPCR) and phylogenetic analysis.
The Blanchot/Beckett Correspondence: Situating the Writer/Writing at the Limen of Naught
Creators: name: Willits, Curt G.
Publisher: name: Monash University
Year: 2017
Subjects: subject: 200599 Literary Studies not elsewhere classified
subject: FOS: Languages and literature

description: Beckett s project to write a literature of the unword corre-sponds to Blanchot s situating the writer/writing at the threshold of the il y a, a term tropologically designating (after Levinas) the passive, indifferent, irreducible naught of Being.
Recruitment of cyanobacteria from the sediments in the eutrophic Shanzi Reservoir
Creators: name: Su, Yuping
name: You, Xuejing
name: Lin, Hui
name: Zhuang, Huiru
name: Weng, Yuan
name: Zhang, Dayi
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Science Policy
subject: Inorganic Chemistry
subject: FOS: Chemical sciences
subject: Biological Sciences
subject: Ecology
subject: FOS: Biological sciences
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Cell Biology
subject: Microbiology

description: This study investigated the impacts of four environmental factors on the recruitment of cyanobacteria from the bottom sediments in the eutrophic Shanzi Reservoir. Temperature and light were identified as the key determinants for the recruitment of Microcystis and Oscillatoria. Cyanobacteria became dominant at higher temperature (20°C) and light intensity (2,000 lx), and Microcystis and Oscillatoria were the major species. Detailed recruitment simulation undertaken with respective gradient of temperature and light suggested that both Microcystis and Oscillatoria are temperature sensitive, and their critical temperature point was 10°C. However, distinct light impacts were observed only on Microcystis. The recruitment of Oscillatoria was light independent, whereas Microcystis had positive relationship with light intensity. Physical disturbance promoted Microcystis recruitment and also affected the structure of recruited cyanobacterial community the water-sediment interaction, based on quantitative polymerase chain reaction (qPCR) and phylogenetic analysis.
Coupled Chemical Reactions in Dynamic Nanometric Confinement: VI. Neutron Depth Profiling Studies of Nanofluidic Behaviour During Track Etching in Polymers
Creators: name: D. Fink
name: J. Vacik
name: V. Hnatowicz
name: G Muñoz, H.
name: H García Arellano
name: A. Kiv
name: L. Alfonta
name: A. Mansharipova
name: R. Muhamediev
Publisher: name: figshare
Year: 2018
Subjects: subject: 30199 Analytical Chemistry not elsewhere classified
subject: FOS: Chemical sciences

description: In recent papers it was shown (by current/voltage (I/V) recordings, Bode plots and Ion Transmission Spectrometry) that the formation of precipitates from solutions in swift heavy ion tracks in thin polymer foils, if coupled with polymer foil etching, eventually leads to the formation of membranes within the etched tracks. Such structures open promising perspectives for the creation of novel biosensors. In this paper we report on Neutron Depth Profiling (NDP) studies of samples where such coupled chemical reactions were initiated in the presence of Li+ and F- ions. Whereas I/V spectroscopy gave us clues about the different migration speeds of both Li+ and F- ions in latent and etched tracks, NDP revealed that Li was always most abundant at the etched track entrances, especially on the track side where the precipitation-forming ions are applied.
Coupled Chemical Reactions in Dynamic Nanometric Confinement: VIII. Capacitive Discharges in Nuclear Track-Based Biosensing
Creators: name: SciDoc Publishers
name: D Fink
name: J Vacik
name: V Hnatowicz
name: S Cruz
name: G Muñoz H
name: H García Arellano
name: A Kiv
name: L Alfonta
name: AT Mansharipova
name: RI Muhamediev7
Publisher: name: figshare
Year: 2017
Subjects: subject: 30199 Analytical Chemistry not elsewhere classified
subject: FOS: Chemical sciences

description: It took quite a time after the independent discoveries of the Leyden jars - the forerunners of our capacitors - in 1745 by Ewald J.G. von Kleist in Pomerania and in 1746 by Pieter van Musschenbroek in Holland, that the capacitor's behaviour upon application of an alternating voltage was understood. As contemporary textbooks reveal, the current I flowing through a capacitor is given by the derivative of the applied voltage U, times the capacitor's capacity C: I = C dU/dt
CLS INFRA D2.1 Communication and Dissemination Plan
Creators: name: Murphy, Ciara L.
name: Tonra, Justin
name: Hoover, Sarah
name: Ridge, Emily
Publisher: name: Zenodo
Year: 2024
Subjects: research communication
research dissemination
research exploitation
computational literary studies

description: D 2.1: Initial plan for communication and dissemination of project results and RI access, including audience/instrument mapping and communications KPIs (M6, to be updated continuously from then on until M 48).
Differential Pulse Polarographic Study of Amoxicillin and Ciprofloxacin and its Determination in Pharmaceuticals
Creators: name: SciDoc Publishers
name: Al-Ameri, Salam A.H.
name: Najlah M.H. Al-Waeli
Publisher: name: figshare
Year: 2017
Subjects: subject: 30199 Analytical Chemistry not elsewhere classified
subject: FOS: Chemical sciences

description: A differential pulse Polarographic method have been developed and validated for the direct determination of amoxicillin and ciprofloxacin in pharmaceuticals. The results show that the optimal analysis conditions for amoxicillin and ciprofloxacin was; the water as a best solvent at neutral medium, pH 7 , while for ciprofloxacin was at acidic medium, pH 4, with BritonRobinson buffer solution and 3M KCl as a supporting electrolyte also 4mm3 mercury drop size at 25°C. Amoxicillin showed two peaks, the first one at - 0.25V and the other one at -0.83V, while the ciprofloxacin appears one peak at the applied potential -1.41V. A standard calibration plot of amoxicillin and ciprofloxacin was prepared in the range between 0.05- 0.525µg.ml-1. The accuracy and precision of the method for the determination of both analyte was tested. The results of laboratory amoxicillin samples showed the SD was 0.08 and RSD% did not exceed 0.13%, while the results of laboratory ciprofloxacin samples illustrate the SD was 0.26 and the RSD% did not exceed 0.4%. The actual value of the peak potential Ep and the number of electrons required for the reduction for amoxicillin and ciprofloxacin was calculated using Ilkovic -Heyrovsky equation, the results confirm the first peak potential Ep was -0.832V with 2 electron used and the second Ep was -0.249V with 1 electron used, while the value of the ciprofloxacin peak potential Ep was -1.38 V with 2 electrons required. This method effectively applied in commercial amoxicillin and ciprofloxacin pharmaceuticals.
Questionnaire destiné aux mères en espagnol
Creators: name: Geffroy, Céline
Publisher: name: NAKALA - https://nakala.fr (Huma-Num - CNRS)
publisherIdentifier: https://ror.org/04ces3204
publisherIdentifierScheme: ROR
Year: 2020

description: Source: http://www.calames.abes.fr/pub/ms/FileId-2009
description: created: ca 2013
Actinotetraose L, a new tetrasaccharide derivative isolated from the mycelia of Amycolatopsis sp. HCa1
Creators: name: Guo, Zhi-Kai
name: Yan, Wei
name: Tan, Ren-Xiang
name: Ge, Hui-Ming
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Biological Sciences
subject: Ecology
subject: FOS: Biological sciences
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Physiology
subject: Molecular Biology
subject: Genetics
subject: Microbiology
subject: Biochemistry

description: A new tetrasaccharide derivative, actinotetraose L (1), was isolated from the methanol extract of the mycelia of the grasshopper-associated rare actinobacterium Amycolatopsis sp. HCa1. The structure of the new compound was elucidated by a combination of 1D, 2D NMR (HMQC, HMBC, COSY, and NOESY), and HR-ESI-MS analyses as O-{3,4-di-O-[(E)-2-ethyl-2-butenoyl]- β-D-glucopyranosyl-(1 → 2)}-O-{3,4-di-O-[(E)-2-methyl-2-butenoyl]-β-D-glucopyranosyl-(1 → 2)}-6,6-di-O-[(E)-2-methyl-2-butenoyl]-α-D-glucopyranosyl-(1 → 1)-α-D-glucopyranoside. The immunosuppressive activity and cytotoxicity of the compound were evaluated by T-cell viability and MTT assays. Nonetheless, no significant activity was observed.
Geochronological results. LA-ICP-MS U–Pb dating of Carboniferous ash layers in the Cantabrian Zone (N Spain): stratigraphic implications
Creators: name: Merino-Tomé, Oscar
name: Gutiérrez-Alonso, Gabriel
name: Villa, Elisa
name: Fernández-Suárez, Javier
name: Llaneza, Jaime Martín
name: Hofmann, Mandy
Publisher: name: Geological Society of London
Year: 2017
Subjects: subject: Geology
subject: FOS: Earth and related environmental sciences

description: SP Table 1. Geochronological results. Concordant ages obtained through LA-ICP-MS.
Digital Citizenship "Active Participation" Course
Creators: name: ANDREI Roxana Elena
name: TEAM4Excellence Association
Publisher: name: Zenodo
Year: 2022
Subjects: Model course

description: The development of technology has created a new social structure that controls who, how and when individuals interact with society. In less than 30 years, the use of the Internet, mobile phones and computers has increased to the point that we hardly know what we would do without them. Digital citizens are required to act in a certain way that fits with accepted norms, standards and regulations.

The Active Participation course is a symbol of independence and democracy. Active participation empowers individuals in the activities and relationships of everyday life leading to them living as independently as possible. Also, it helps individuals to increase autonomy, self-confidence and self-esteem. Moreover, it refers to the involvement of people in their society, by taking initiatives based on their needs, showing that citizens are not passive and they do care.

This course was designed as an instrument to assist teachers, trainers and youth workers in their daily work with young people. However, the course may be also followed directly by young people on their own. It includes readings, exercises, case studies, quizzes and self-reflection questions, divided into five modules:

Digital citizens in a digital world;

Make your youth remarkable;

The freedom of voting;

Advocacy: networking for advocacy strategy;

Online support with real impact.

While this Active Participation textbook is available to download for free here and in the TrainingClub.eu European non-formal education platform of TEAM4Excellence, it is also available as a Moodle massive open online course in English. Furthermore, each module was translated into three languages: Romanian, Greek and German.

“Active Participation” is the result of the Erasmus+ project “Strategic partnership to develop open educational resources for teaching digital citizenship”, implemented by a consortium of four partners from Romania, Cyprus, Germany and Greece, aiming at improving a broad range of competences, so citizens are able to actively, positively and responsibly engage in both on and offline communities.

The authors consider that digital citizenship should be faced as a multidimensional and complex concept. Based on this aspect, they created 10 courses that together help improve the digital citizenship skills of people: Access and Inclusion, Learning and Creativity, Media and Information Literacy, Ethics and Empathy, Health and Wellbeing, ePresence and Communication, Active Participation, Rights and Responsibilities, Privacy and Security, Consumer Awareness.

Besides English, each course was translated into three more languages: Romanian, Greek and German. All the courses may be accessed online and anyone can enrol on Moodle and follow the courses for free.
description: Grant Agreement 2019-3-RO01-KA205-078053, Strategic partnership to develop open educational resources for teaching digital citizenship (DIGCIT)
Evaluation of Travis County Investments in Workforce Development 2019 Update
Creators: name: Juniper, Cynthia
name: Rodriguez, Patty
name: Prince, Heath
Publisher: name: Ray Marshall Center for the Study of Human Resources
Year: 2019
Subjects: Workforce Development
Labor Market and Industry Studies

description: This evaluation examines outcomes and impacts for participants in the Travis County-funded community-based workforce programs exiting services in FY 2016–FY 2018. To understand the impact of these services, the county has contracted with the Ray Marshall Center for the Study of Human Resources (RMC) at the University of Texas at Austin to conduct a longitudinal evaluation of its investments. This workforce development evaluation report presents the analysis of outcomes and estimated net impacts of these investments in the programs funded the first three years of a five year on-going evaluation (FY 2016–FY 2020). The first section presents an overview of the evaluation questions and research methods, followed by separate sections for each of the providers examined. Each provider section includes a brief profile of the provider and its workforce development program(s), a summary of participant demographic characteristics obtained at the time of program entry, and details outcomes and impacts for participants who exited the program during FY 2016–FY 2018. Findings examine Unemployment Insurance (UI) wage data for the quarter the client exited services, four quarters prior to entering the program, and up to 12 quarters post-exit. Findings examine results in the post-service period through March 31, 2019, the latest quarter for which UI wage data are available. The final section summarizes evaluation findings from FY 2016–FY 2018. Outcomes and impacts vary across the spectrum of grantees as would be expected given their varying services regimes, and the unique barriers to training and employment experienced by the target populations each organization serves.
Coupled Chemical Reactions in Dynamic Nanometric Confinement: VI. Neutron Depth Profiling Studies of Nanofluidic Behaviour During Track Etching in Polymers
Creators: name: SciDoc Publishers
name: D Fink
name: J Vacik
name: V Hnatowicz
name: G Muñoz H
name: H García Arellano
name: A Kiv
name: L Alfonta
name: A Mansharipova
name: R Muhamediev
Publisher: name: figshare
Year: 2017
Subjects: subject: 30199 Analytical Chemistry not elsewhere classified
subject: FOS: Chemical sciences

description: In recent papers it was shown (by current/voltage (I/V) recordings, Bode plots and Ion Transmission Spectrometry) that the formation of precipitates from solutions in swift heavy ion tracks in thin polymer foils, if coupled with polymer foil etching, eventually leads to the formation of membranes within the etched tracks. Such structures open promising perspectives for the creation of novel biosensors. In this paper we report on Neutron Depth Profiling (NDP) studies of samples where such coupled chemical reactions were initiated in the presence of Li+ and F- ions. Whereas I/V spectroscopy gave us clues about the different migration speeds of both Li+ and F- ions in latent and etched tracks, NDP revealed that Li was always most abundant at the etched track entrances, especially on the track side where the precipitation-forming ions are applied.
Validated HPLC Method for the Determination of Fenofibric Acid in Rat Plasma and its Application to a Comparative Pharmacokinetic Study of Prodrugs JWU102 and Fenofibrate
Creators: name: SciDoc Publishers
name: TK, Kim
name: OH, Jung
Publisher: name: figshare
Year: 2017
Subjects: subject: 30199 Analytical Chemistry not elsewhere classified
subject: FOS: Chemical sciences

description: In this study, a sensitive and reliable method for the quantitation of fenofibric acid in rat plasma was developed and validated using high performance liquid chromatography (HPLC). The plasma samples were prepared by deproteinization, and sildenafil was used as an internal standard. Chromatographic separation was achieved using a reversed-phase (C18) column. The mobile phase, 0.02 M ammonium acetate buffer: acetonitrile (35:65, v/v), was run at a flow rate of 1.0 mL/min, and the column eluent was monitored using an ultraviolet detector at 280 nm at room temperature. The retention times of sildenafil (an internal standard), and fenofibric acid were approximately 5.9 and 7.7 min, respectively. The quantitation limit of fenofibric acid in rat plasma was 0.03 μg/mL. Pharmacokinetic parameters of fenofibric acid was evaluated after oral (at doses of 20 mg/kg) administration of JWU102 and fenofibrate in rats. The relative bioavailability of JWU102 was approximately 272.8% compared to fenofibrate
SP Fig. 2. LA-ICP-MS U–Pb dating of Carboniferous ash layers in the Cantabrian Zone (N Spain): stratigraphic implications
Creators: name: Merino-Tomé, Oscar
name: Gutiérrez-Alonso, Gabriel
name: Villa, Elisa
name: Fernández-Suárez, Javier
name: Llaneza, Jaime Martín
name: Hofmann, Mandy
Publisher: name: Geological Society of London
Year: 2017
Subjects: subject: Geology
subject: FOS: Earth and related environmental sciences

description: SP FIg. 2. Stratigraphic section of the Santo Firme Coalfield reconstructed using information from IGME (1984) and Trell (1988). Fusuline Zones from Villa & Merino-Tomé (2016), palynologic Zones from Horvarth (1985) and stages and substages of Western European regional scale and Uralsare indicated.
title: 03_0868
Creators: name: Wesley Baldwin, John
name: Baldwin, John W.
Publisher: name: NAKALA - https://nakala.fr (Huma-Num - CNRS)
publisherIdentifier: https://ror.org/04ces3204
publisherIdentifierScheme: ROR
Year: 2018
Subjects: subject: LaMOP
subject: Laboratoire de Médiévistique Occidentale de Paris

description: L'ensemble de ces notes ont été crée par John Baldwin lors d'une de ses premières périodes de résidence à Paris. Ces documents comprennent tous les magistri qu’il avait pu rencontrer dans ses sources. Aux XIIème et XIIIème siècles, les universités n'existant pas encore, le titre de magister s'il évoque certainement une compétence n'est pas rattaché de façon systématique au passage par les écoles. De surcroît, les magistris sont le plus souvent désignés par leur seul prénom. La lecture de ces données est donc difficile et suppose un long travail pour tenter d'identifier un individu auquel on pourrait rattacher plusieurs mentions. John Baldwin avait donc renoncé à utiliser ces données pour donner un prolongement à sa magnifique étude sur les gradués d'université et leur emploi par les Capétiens et les Plantagenêts. Néanmoins, il a conservé ces données et elles sont potentiellement très utile dans le cadre de Studium, comme le sont toutes les mentions dans les volumes des Fasti Ecclesiae Gallicanae de chanoines portant ce titre de magister sans autres précisions. L'ampleur des dépouillements rend ces données très utiles et nous devons une grande reconnaissance à John Baldwin pour avoir pris la peine de faire photocopier ses fiches et de nous les avoir transmises amicalement.
Les données ainsi que les images des fiches sont accessibles sur le site Internet à l'adresse suivante : http://studium-baldwin.lamop.fr/.
Les transcriptions des fiches ont été effectuées par des étudiants de Paris 1 Panthéon - Sorbonne mais elles n'ont pas été corrigées par la suite.
description: Relation: https://lamop.hypotheses.org/
Professional Closure: An Example from American Clinical Psychology
Creators: name: Merced, Matthew
Publisher: name: Advanced Journal of Professional Practice
Year: 2019

description: Objective: Study the American clinical psychology landscape to discern scientist-practitioner and practitioner-scholar involvement in professional activities, specifically the organizational and scholarly domains. Methods: Data were gathered from online sources regarding: professional association membership, leadership, and award recipients; faculty positions within psychology doctoral programs; journal editor positions; and contributions to the scholarly literature. Results: Scientist-practitioners dominate American clinical psychology’s principal professional association’s membership (73.5%) and leadership (93.2%) and receive nearly all its awards (98.2%). Faculties for both practitioner-scholar programs (76.2%) and scientist-practitioner programs (99.1%) were dominated by scientist-practitioners. The editor of each journal surveyed was a scientist-practitioner. Most literature contributions (77.3%) were from scientist-practitioners. Conclusions: Scientist-practitioners in the United States can access organizational and scholarly roles/activities. Practitioner-scholars do not have the same access. Scientist-practitioners use implicit, normalized practices known as ‘closure methods’ to preserve their access to professional opportunities, resources, and rewards. Practitioner-scholars, subject to ‘professional closure’, compose an excluded group.
description: Advanced Journal of Professional Practice, Vol 2 No 1 (2019)
Causes of increased reproductive performance in Dummerstorf high-fertility mouse lines, FL1 and FL2
Creators: name: Calanni Pileri, Michela
Publisher: name: Universität Rostock
Year: 2023
Subjects: subject: 500 Natural sciences
subject: 570 Life science
subject: 610 Medical sciences Medicine

description: Dummerstorf high fertility mouse lines are selected for increased fertility. After ~200 generations they doubled their ovulation rate and litter size. This worldwide unique lines can give some insides into the desired highly-informative phenotype of high-fertility, in which those peculiarities of enhanced reproductive performance coexist with characteristics that the literature mostly correlates with a lowering of fertility. The deep analysis of FLs can be a very informative way to get the methods they use to avoid their "bad" characteristics to have an impact in their higher-fertility traits.
Efficient PRNP deletion in bovine genome using gene-editing technologies in bovine cells
Creators: name: Choi, WooJae
name: kim, EunJi
name: Yum, Soo-Young
name: Lee, ChoongIl
name: Lee, JiHyun
name: Moon, JoonHo
name: Ramachandra, Sisitha
name: Oshadi Malaweera, Buddika
name: Cho, Jongki
name: Kim, Jin-Soo
name: Kim, SeokJoong
name: Jang, Goo
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Microbiology
subject: FOS: Biological sciences
subject: Hematology
subject: Cancer
subject: Developmental Biology
subject: Biological Sciences
subject: Molecular Biology
subject: Genetics
subject: Cell Biology
subject: Engineering

description: ABSTRACTEven though prion (encoded by the PRNP gene) diseases like bovine spongiform encephalopathy (BSE) are fatal neurodegenerative cattle in cattle, their study via gene deletion has been limited due to the absence of cell lines or mutant models. In this study, we aim to develop an immortalized fibroblast cell line in which genome-engineering technology can be readily applied to create gene-modified clones for studies. To this end, this study is designed to 1) investigate the induction of primary fibroblasts to immortalization by introducing Bmi-1 and hTert genes; 2) investigate the disruption of the PRNP in those cells; and 3) evaluate the gene expression and embryonic development using knockout (KO) cell lines. Primary cells from a male neonate were immortalized with Bmi-1 and hTert. Immortalized cells were cultured for more than 180 days without any changes in their doubling time and morphology. Furthermore, to knockout the PRNP gene, plasmids that encode transcription activator-like effector nuclease (TALEN) pairs were transfected into the cells, and transfected single cells were propagated. Mutated clonal cell lines were confirmed by T7 endonuclease I assay and sequencing. Four knockout cell lines were used for somatic cell nuclear transfer (SCNT), and the resulting embryos were developed to the blastocyst stage. The genes (CSNK2A1, FAM64A, MPG and PRND) were affected after PRNP disruption in immortalized cells. In conclusion, we established immortalized cattle fibroblasts using Bmi-1 and hTert genes, and used TALENs to knockout the PRNP gene in these immortalized cells. The efficient PRNP KO is expected to be a useful technology to develop our understanding of in vitro prion protein functions in cattle.
Supplementary material. LA-ICP-MS U–Pb dating of Carboniferous ash layers in the Cantabrian Zone (N Spain): stratigraphic implications
Creators: name: Merino-Tomé, Oscar
name: Gutiérrez-Alonso, Gabriel
name: Villa, Elisa
name: Fernández-Suárez, Javier
name: Llaneza, Jaime Martín
name: Hofmann, Mandy
Publisher: name: Geological Society of London
Year: 2017
Subjects: subject: Geology
subject: FOS: Earth and related environmental sciences

description: Stratigraphic position and biostratigraphic information on the studied ash-fall layers, and analytical methods and geochronological results.
title: Carnet de poésie
title: Carnet de poésie
Creators: name: Le Braz, Anatole (1859-1926)
Publisher: name: NAKALA - https://nakala.fr (Huma-Num - CNRS)
publisherIdentifier: https://ror.org/04ces3204
publisherIdentifierScheme: ROR
Year: 2021

description: Source: Fonds Anatole Le Braz. Centre de Recherche Bretonne et Celtique (CRBC). Bibliothèque Yves-Le Gallo.
description: Provenance: Ce document provient du fonds Anatole Le Braz conservé au Centre de recherche bretonne et celtique. Les documents ont été déposés en 1986 par l’un des petits-enfants d’Anatole Le Braz et par Yann-Ber Piriou en 1999.
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description: IIIF manifest
The influence of Internet Social Media on Professional Practice: A Case Study of Perceptions of Foundation Dentists in Health Education England, Kent, Surrey and Sussex
Creators: name: Hardy, Raymond
name: Parkin, Claire L
Publisher: name: Advanced Journal of Professional Practice
Year: 2018

description: Social mediais a popular, technology-led form of communication. It has a developing but contentious role in healthcare professional practice, attracting interest and opinion from official bodies, academic institutions and terrestrial and internet media. This project explored Health Education England Kent, Surrey and Sussex (HEE KSS) Foundation Dentists’ (FDs) perceptions of the influence of internet social media on their professional practice.
description: Advanced Journal of Professional Practice, Vol 1 No 2 (2018)
SP Fig. 3. LA-ICP-MS U–Pb dating of Carboniferous ash layers in the Cantabrian Zone (N Spain): stratigraphic implications
Creators: name: Merino-Tomé, Oscar
name: Gutiérrez-Alonso, Gabriel
name: Villa, Elisa
name: Fernández-Suárez, Javier
name: Llaneza, Jaime Martín
name: Hofmann, Mandy
Publisher: name: Geological Society of London
Year: 2017
Subjects: subject: Geology
subject: FOS: Earth and related environmental sciences

description: SP Fig. 3. Synthetic stratigraphic section of the Caleras, Generalas, San Antonio, Sotón and Entrerregueras stratigraphic packages, reconstructed using stratigraphic information provided by Hunosa, showing the location of fusuline samples studied by Van Ginkel (1973) (white colour circles), Barba (1983) (grey colour circles) and in this study (black colour circles). Local names of mined coal seams and reference limestone, sandstone and conglomerate stratigraphic levels are also indicated. See Fig. 5 for index and a key of symbols.
A preposterior analysis to predict identifiability in the experimental calibration of computer models
Creators: name: D. Arendt, Paul
name: W. Apley, Daniel
name: Chen, Wei
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Neuroscience
subject: Cell Biology
subject: Plant Biology
subject: FOS: Biological sciences
subject: Cancer
subject: Mathematics
subject: FOS: Mathematics
subject: Information and Computing Sciences
subject: Biological Sciences
subject: Earth and Environmental Sciences
subject: Biotechnology
subject: Genetics
subject: Medicine

description: ABSTRACTWhen using physical experimental data to adjust, or calibrate, computer simulation models, two general sources of uncertainty that must be accounted for are calibration parameter uncertainty and model discrepancy. This is complicated by the well-known fact that systems to be calibrated are often subject to identifiability problems, in the sense that it is difficult to precisely estimate the parameters and to distinguish between the effects of parameter uncertainty and model discrepancy. We develop a form of preposterior analysis that can be used, prior to conducting physical experiments but after conducting the computer simulations, to predict the degree of identifiability that will result after conducting the physical experiments for a given experimental design. Specifically, we calculate the preposterior covariance matrix of the calibration parameters and demonstrate that, in the examples that we consider, it provides a reasonable prediction of the actual posterior covariance that is calculated after the experimental data are collected. Consequently, the preposterior covariance can be used as a criterion for designing physical experiments to help achieve better identifiability in calibration problems.
Doing Research in the Real World
Creators: name: Brennan, Anne-Maria
Publisher: name: Advanced Journal of Professional Practice
Year: 2018

description: David E. Gray has produced a 4th edition of his successful textbook Doing Research in the Real World. This useful handbook for social science researchers includes the addition of entirely new chapters on Visual Research Methods, Digital Research Methods and Getting Started Using NVIVO, along with other chapters updated to reflect developments in digital research tools. This has been done whilst also retaining a comprehensive overview of research methods in the social sciences. The purpose of this book remains to assist researchers and their supervisors in navigating the complex decisions involved in the design and execution of research projects and does this in a clear and approachable way.
description: Advanced Journal of Professional Practice, Vol 1 No 2 (2018)
The perceived strengths and weaknesses of the General Practice training e-portfolio: A case study exploration of General Practitioner (GP) trainers’ perspectives
Creators: name: Elliot, Jane
name: Reed, Debbie
Publisher: name: Advanced Journal of Professional Practice
Year: 2018

description: Background : General Practitioner (GP) trainers[1] spend considerable time completing their trainees’ e-portfolios, yet there is a paucity of research into their views This study aimed to illuminate their perspectives and propose modifications. Additionally, a recent law-suit has highlighted tensions over written reflections in training e-portfolios being used in a court of law and this paper contributes to the conversation Methods: Case study methodology was adopted. A survey permitted purposeful selection of six GP trainers for interview and informed the interview schedule. Semi-structured interviews provided the data and thematic analysis was employed for data analysis. Credibility indicators included member-checking and cross-checking. Results: Strengths and weaknesses of the e-portfolio were identified. Strengths lay in the ability to demonstrate accountability for a rigorous educational process, and intrinsic educational aspects of the e-portfolio. Weaknesses lay in the time spent by GP trainers in documentation, perceived by them as excessive, and the threats to credibility conferred both by burdensome documentation and the requirement for written reflection on clinical errors. Conclusions and Recommendations: There is a risk of GP trainers endangering their work-life balance and clinical performance by the time spent on the e-portfolio. Participants proposed reducing documentation with fewer competencies and log-entries. They suggested that written reflection on clinical imperfections should not be expected, whilst learning from researching knowledge gaps should, and that they, as GP trainers, should be more involved in e-portfolio evolution. [1] GP trainers are GPs who have undergone extra training in education, often involving obtaining a Post Graduate Certificate in Education.
description: Advanced Journal of Professional Practice, Vol 1 No 2 (2018)
A Preposterior Analysis to Predict Identifiability in Experimental Calibration of Computer Models
Creators: name: D. Arendt, Paul
name: W. Apley, Daniel
name: Chen, Wei
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Neuroscience
subject: Cell Biology
subject: Plant Biology
subject: FOS: Biological sciences
subject: Cancer
subject: Mathematics
subject: FOS: Mathematics
subject: Information and Computing Sciences
subject: Biological Sciences
subject: Earth and Environmental Sciences
subject: Biotechnology
subject: Genetics
subject: Medicine

description: When using physical experimental data to adjust, or calibrate, computer simulation models, two general sources of uncertainty that must be accounted for are calibration parameter uncertainty and model discrepancy. This is complicated by the well-known fact that systems to be calibrated are often subject to identifiability problems, in the sense that it is difficult to estimate the parameters precisely and to distinguish between the effects of parameter uncertainty versus model discrepancy. We develop a form of preposterior analysis that can be used, prior to conducting physical experiments but after conducting the computer simulations, to predict the degree of identifiability that will result after conducting the physical experiments for a given experimental design. Specifically, we calculate the preposterior covariance matrix of the calibration parameters and demonstrate that, in the examples that we consider, it provides a reasonable prediction of the actual posterior covariance that is calculated after the experimental data are collected. Consequently, the preposterior covariance can be used as a criterion for designing physical experiments to help achieve better identifiability in calibration problems. Supplementary materials are available for this article at the publisher's online edition of IIE Transaction, datasets, additional tables, detailed proofs, etc.
Identification of the perceived ongoing Continuing Professional Development needs of six dentists at the end of Dental Foundation Training with Health Education Kent Surrey and Sussex
Creators: name: Grossman, Alina
name: Reed, Debbie
Publisher: name: Advanced Journal of Professional Practice
Year: 2018

description: Aim: This project aimed to identify the ongoing Continuing Professional Development (CPD) needs of six dentists at the end of Dental Foundation Training (DFT) with Health Education Kent, Surrey and Sussex (HEKSS), in relation to the gap between self-perceived and optimum competence levels across a range of learning areas in the DFT curriculum. Method: Qualitative Case Study methodology was used. Six dentists were interviewed by the investigator using Repertory Grid based semi-structured interviews. Data was analysed by thematic analysis. Results: Dentists perceived that CPD activities were required to develop knowledge, skill, attitudes and behaviour. Other supportive CPD activities were perceived to aid this development. Individual circumstances were perceived to either facilitate or impede opportunities for development. Conclusion: A range of CPD activities are required at the end of DFT, including passive learning activities, practical experience, practical courses, exposure to a range of patients, and support and guidance from colleagues. Individual circumstances may be barriers to, or opportunities for, securing such CPD activities.
description: Advanced Journal of Professional Practice, Vol 1 No 2 (2018)
A novel approach to resuspend particles of controlled morphologies in a 3D printed wind tunnel
Creators: name: Neal, Edward
name: Mahato, Lukesh K.
name: Butler, Elin
name: Thomas, Richard J.
name: Walker, Maurice D.
name: Vincent, Jack C.
name: Parker, Simon T.
name: Foot, Virginia E.
name: Kruger, Emily S.
name: Higgins, Benjamin
name: Sellors, William
name: Reid, Jonathan P.
Publisher: name: Taylor & Francis
Year: 2024
Subjects: subject: Biophysics
subject: Biotechnology
subject: Ecology
subject: FOS: Biological sciences

description: Particle resuspension is an omnipresent source of aerosols into the environment as the necessary detachment force to return particles to the atmosphere can arise from a variety of environmental and anthropogenic mechanisms. Despite the significance of resuspension, the phenomenon is often neglected in aerosol dispersion models as there is limited understanding on the magnitude of the effect and its influencing factors. We provide a route toward determining the role of particle-specific characteristics, such as morphology, on resuspension efficiency with the aim of introducing greater complexity to resuspension models. A combination of two unique instruments is introduced, a quadrupole electrodynamic trap to fabricate particles and a small-scale 3D printed wind tunnel. Together they provide a method for manufacturing and resuspending particles of a reproducible spherical and non-spherical shape without the need for large or costly equipment. Experiments resuspending spherical glass standards and raspberry-shaped dried sodium chloride particles, alongside anemometer flow field analysis, were implemented to validate the approach and provide insight into potential limitations, including the necessity to increase non-spherical particle resuspension rates to evaluate the impacts of morphology in the future. This provides the foundation for future experiments with increasing deviations from idealized conditions and prospective step-wise resuspension model developments.
Insights into the enhancement of nanoparticle production throughput by atmospheric-pressure spark ablation
Creators: name: Loizidis, C.
name: Petallidou, K. C.
name: Maisser, A.
name: Bezantakos, S.
name: Pfeiffer, T. V.
name: Schmidt-Ott, A.
name: Biskos, G.
Publisher: name: Taylor & Francis
Year: 2024
Subjects: subject: Biophysics
subject: Biochemistry
subject: Space Science
subject: Physical Sciences not elsewhere classified
subject: Medicine
subject: Neuroscience
subject: Biotechnology
subject: Ecology
subject: FOS: Biological sciences
subject: Biological Sciences not elsewhere classified

description: Spark ablation is a highly effective and versatile method for producing nanoparticles from bulk conductive electrode materials. For a number of applications, however, the production throughput of the process needs to be increased with respect to the current state of the art. Here we show that this can be achieved by decreasing the diameter of the employed bulk-material electrodes from ca. 12 to 0.15 mm, corroborating previous observations, and demonstrate that the throughput is associated with the ablation efficiency (i.e., the energy spent to produce nanoparticles per total input energy) that respectively increases by a factor of 10. It is also shown that the commonly used theory for predicting the mass of nanoparticles produced by spark ablation cannot capture this effect, and thus we extend it to account for heat losses that affect the process when electrode diameter reduces below ca. 2 mm. Through this exercise we also show that reduced heat losses associated with thinner electrodes provide an effective recipe to increase the ablation efficiency, also referred as the nanoparticle production yield. The new extended theory for estimating spark ablation nanoparticle mass production throughput is also accompanied by an empirical equation predicting its dependence on electrode diameter.
SP Fig. 4. LA-ICP-MS U–Pb dating of Carboniferous ash layers in the Cantabrian Zone (N Spain): stratigraphic implications
Creators: name: Merino-Tomé, Oscar
name: Gutiérrez-Alonso, Gabriel
name: Villa, Elisa
name: Fernández-Suárez, Javier
name: Llaneza, Jaime Martín
name: Hofmann, Mandy
Publisher: name: Geological Society of London
Year: 2017
Subjects: subject: Geology
subject: FOS: Earth and related environmental sciences

description: SP Fig. 4. Synthetic stratigraphic section of the Puerto Ventana Stephanian succession at the Bienvenida Mine reconstructed using information of the zero, first and second levels in the mine using information of Enadimsa (1981) report. See Fig. 5 for index and a key of symbols.
Design, evaluation, and performance of versatile source sampling system (VS3) for monitoring and characterization of aerosols from area, point and line sources
Creators: name: Kumari, Jyoti
name: Khan, Mohd Shahzar
name: Bansal, Mahak
name: Kapoor, Taveen
name: Habib, Gazala
Publisher: name: Taylor & Francis
Year: 2024
Subjects: subject: Biophysics
subject: Biochemistry
subject: Space Science
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Biotechnology
subject: Environmental Sciences not elsewhere classified
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: Science Policy

description: The aerosol emission measurement at sources is crucial for effective environmental regulations and upgradation to global air quality standards. In the present study a Versatile Source Sampling System (VS3) has been designed, fabricated and evaluated to capture emissions from various sources. This study addresses key operational challenges such as isokinetic conditions for aerosol collection, mimicking rapid exhaust and zero-air mixing for gas-to-particle partitioning, and maintaining uninterrupted power supply. Depending upon the emission source, i.e., area, line or point, the aerosol sampling probes and a portable ejector dilutor with dilution tunnel were designed and evaluated in the laboratory and on-field for mixing and particle losses at three dilution ratios 20:1, 30:1, and 40:1. Particle loss experiments were done with sodium chloride and polystyrene latex aerosols. The on-field measurements were carried out with diesel generator, truck and light motor vehicle-goods. The overall particle losses were found to be 8% in the inlet designed for area source measurement, 1-3% for the ejector with dilution tunnel, and 9% and 13% for line and point source sampling inlets, respectively. There was no significant change (p-value > 0.05) in the average particle geometric mean diameter inside the dilution tunnel at different dilution ratios indicating no coagulation. For the first time, this study has reported the PM2.5 (particles with aerodynamic diameter <2.5 µm) emission factor of kerosene fuel used in diesel generator in rural areas for commercial use with values identified between 44 ± 16 gkg−1 accounting 30% Organic Carbon (OC) and 16% Elemental Carbon (EC).