Additional file 2 of Combined morpho-physiological, ionomic and transcriptomic analyses reveal adaptive responses of allohexaploid wheat (Triticum aestivum L.) to iron deficiency
Creators: name: Hua, Ying-peng
name: Wang, Yue
name: Zhou, Ting
name: Huang, Jin-yong
name: Yue, Cai-peng
Publisher: name: figshare
Year: 2022
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: Plant Biology

description: Additionalfile 2: Figure S1. Correlation analysis of shoot and root samples. HIR:High Iron Root, HIS: High Iron Shoot, LIR: Low Iron Root, LIS: Low Iron Shoot.1, 2, and 3 respectively represent different biological repetitions. Figure S2. Genomic distribution ofdifferentially expressed genes (DEGs). Genomic distribution of DEGs in the shoots (A)and roots (B). Figure S3. Molecularmodel of Fe absorption and transport related genes. A molecular model of genesinvolved in Fe absorption and transport in plant roots (A), chloroplasts,mitochondria, and vacuoles (B). FigureS4. Differential expression profiles of genes related to Fe absorption andtransport in wheat plants under low Fe stress. (A) Fe3+ chelatereductase (FRO), (B) naturalresistance-associated macrophage protein (NRAPM),(C) Yellow stripe-like (YSL), (D) methionine synthetase (SAM), (E) iron-regulated transporter (IRT), (F) Deoxyergate synthase (DMAS), (G) Nicotinamide aminotransferase(NAAT), (H) Mitochondrial m-typethioredoxin in chloroplast (ATM), (I)Nicotinamide synthase (NAS), (J)transporter of mugnetic acid (TOM),(K) multidrug and toxin efflux family (MATE),(L) Permease in chioroplasts (PIC),(M) Oligopeptide transporter (OPT),(N) mitoferrin, (O) mitoferrin-like, (P) Iron efflux transporter ferroportin (FPN), (Q) Nonintrinsic ABC protein (NAP), (R) vacuolar iron transporterginseng (VIT). For transcriptomesequencing, selected uniform wheat plants after germination, half of which weretransplanted into a nutrient solution with normal Fe concentration forcultivation, and half were transplanted into a nutrient solution with low Fefor cultivation, and samples were taken 10 days later. The heat map shows thegene expression level indicated by the TPM value. Differentially expressedgenes that show higher expression levels under the control (normal Fe: 50 μM)and treatment (low Fe: 2 μM) are indicated by asterisks. Figure S5. Differential expression profile ofphotosynthesis-related genes in wheat plants under low Fe stress. Differentialexpression profile of genes related to photosynthesis-antenna proteins (A) andcarotenoid biosynthesis (B). The expression pattern of genes involved in thetransformation of chlorophyll a and chlorophyll b (C) and the synthesis ofcarotenoids under low Fe stress (D). The expression pattern of chlorophyll a/bbinding proteins (E), Photosynthesis II reaction proteins (F), RuBisCo subunitbinding proteins (G), Mg2+ chelatase (H), Ribulose bisphosphatecarboxylase/oxygenase activase (I). For transcriptome sequencing, selecteduniform wheat plants after germination, half of which were transplanted into anutrient solution with normal Fe concentration for cultivation, and half weretransplanted into a nutrient solution with low Fe for cultivation, and sampleswere taken 10 days later. The heat map shows the gene expression levelindicated by the TPM value. Differentially expressed genes that show higherexpression levels under he control (normal Fe: 50 μM) and treatment (low Fe: 2μM) are indicated by asterisks. FigureS6. Heat map of expression of genes involved incell cycle and ROS metabolism. (A) Graph of expression abundance of cellcycle-related genes. Differential expression profile of ROS synthesis (B) andclearance related genes (C). For transcriptome sequencing, selected uniformwheat plants after germination, half of which were transplanted into a nutrientsolution with normal Fe concentration for cultivation, and half weretransplanted into a nutrient solution with low Fe for cultivation, and sampleswere taken 10 days later. The heat map shows the gene expression levelindicated by the TPM value. Differentially expressed genes that show higher expressionlevels under he control (normal Fe: 50 μM) and treatment (low Fe: 2 μM) areindicated by asterisks. Figure S7. Co-expressionnetwork analysis of Fe absorption and transport related genes under low Festress conditions. Cycle nodes represent genes, and the size of the nodesrepresents the power of the interrelation among the nodes by degree value.Edges between two nodes represent interactions between genes. Figure S8. Natural variation in wheatresistance to low Fe stress and morphological identification of resistant andsensitive genotypes. (A) Variation in low Fe stress in a panel comprising 386wheat accessions. The root length and chlorophyll value of wheat were used forpopulation screening. After germination, uniform seedlings were selected andgrown for 10 d in Hoagland nutrient solution. Then Half of the seedlings werecultivated in a nutrient solution containing sufficient Fe, and half werecultivated in a nutrient solution containing low Fe stress. Min, minimum; max,maximum; CV, coefficient of variation. (B) Growth performance of ‘zhengmai1860’and ‘zhoumai32’ under low Fe stress. For the low Fe treatment, the wheat plantswere grown hydroponically under high Fe (50 μM) and low Fe (2 μM) conditionsfor 10 d, respectively. Bar: 5cm. (C) Relative expression of TaIRT1b-4A,TaNAS2-6D, TaNAS1a-6A, TaNAS1a-6B, and TaNAAT1b-1D in the shoots and roots ofthe two genotypes grown under normal Fe and low Fe conditions. For the low Fetreatment, the wheat plants were grown hydroponically under high Fe (50 μM) andlow Fe (2 μM) conditions for 10 d, respectively. Data are means (±SD), n=3. Thesignificant difference was determined using Student’s t-test: **P&lt;0.01. Table S2. RT-qPCR primersequences.