Transcriptome-based identification of antioxidative gene expression after fish oil supplementation in normo- and dyslipidemic men
Creators: name: Schmidt, Simone
name: Stahl, Frank
name: Mutz, Kai-Oliver
name: Scheper, Thomas
name: Hahn, Andreas
name: Schuchardt, Jan Philipp
Publisher: name: London : BioMed Central Ltd.
Year: 2012
Subjects: subject: Antioxidative defence
subject: Catalase
subject: Cytochrome P450 enzyme
subject: Dyslipidemia
subject: Glutathione
subject: Heme oxygenase
subject: Matrix metalloproteinase
subject: Omega-3 fatty acids
subject: Oxylipines
subject: catalase
subject: cytochrome P450
subject: cytochrome P450 1A2
subject: docosahexaenoic acid
subject: gelatinase A
subject: glutathione reductase
subject: glutathione transferase
subject: heme oxygenase 2
subject: icosapentaenoic acid
subject: immunoglobulin enhancer binding protein
subject: matrix metalloproteinase
subject: matrix metalloproteinase 25
subject: mitogen activated protein kinase
subject: omega 3 fatty acid
subject: stromelysin
subject: superoxide dismutase
subject: transcriptome
subject: unclassified drug
subject: adult
subject: antioxidant activity
subject: article
subject: clinical article
subject: controlled study
subject: down regulation
subject: drug effect
subject: dyslipidemia
subject: erythrocyte membrane
subject: fatty acid analysis
subject: gene control
subject: gene expression
subject: genetic transcription
subject: heart protection
subject: human
subject: male
subject: microarray analysis
subject: nucleotide sequence
subject: oxidative stress
subject: randomized controlled trial
subject: real time polymerase chain reaction
subject: upregulation
subject: Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie
subject: Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit

description: Background: The beneficial effects of omega-3 polyunsaturated fatty acids (n-3 PUFAs), especially in dyslipidemic subjects with a high risk of cardiovascular disease, are widely described in the literature. A lot of effects of n-3 PUFAs and their oxidized metabolites are triggered by regulating the expression of genes. Currently, it is uncertain if the administration of n-3 PUFAs results in different expression changes of genes related to antioxidative mechanisms in normo- and dyslipidemic subjects, which may partly explain their cardioprotective effects. The aim of this study was to investigate the effects of n-3 PUFA supplementation on expression changes of genes involved in oxidative processes. Methods: Ten normo- and ten dyslipidemic men were supplemented for twelve weeks with fish oil capsules, providing 1.14 g docosahexaenoic acid and 1.56 g eicosapentaenoic acid. Gene expression levels were determined by whole genome microarray analysis and quantitative real-time polymerase chain reaction (qRT-PCR). Results: Using microarrays, we discovered an increased expression of antioxidative enzymes and a decreased expression of pro-oxidative and tissue enzymes, such as cytochrome P450 enzymes and matrix metalloproteinases, in both normo- and dyslipidemic men. An up-regulation of catalase and heme oxigenase 2 in both normo- and dyslipidemic subjects and an up-regulation of cytochrome P450 enzyme 1A2 only in dyslipidemic subjects could be observed by qRT-PCR analysis. Conclusions: Supplementation of normo- and dyslipidemic subjects with n-3 PUFAs changed the expression of genes related to oxidative processes, which may suggest antioxidative and potential cardioprotective effects of n-3 PUFAs. Further studies combining genetic and metabolic endpoints are needed to verify the regulative effects of n-3 PUFAs in antioxidative gene expression to better understand their beneficial effects in health and disease prevention.