title: Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð¼ÐµÑÐ¾Ð´Ð¸Ðº Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Ð½Ð°Ð½Ð¾- Ð¸ Ð¼Ð¸ÐºÑÐ¾Ð½Ð¾ÑÐ¸ÑÐµÐ»ÐµÐ¹ Ð´Ð»Ñ in vivo Ð¸ in vitro Ð²Ð¸Ð·ÑÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Development of methods for modification of nano- and micron carriers for in vivo and in vitro visualization
Creators: name: ÐÑÐ¼ÐµÑÐ¾Ð²Ð°, ÐÐ°ÑÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: Ð½Ð°Ð½Ð¾Ð±Ð¸Ð¾ÑÐµÑ Ð½Ð¾Ð»Ð¾Ð³Ð¸Ð¸
Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ½ÑÐµ Ð¼Ð¸ÐºÑÐ¾Ð½Ð¾ÑÐ¸ÑÐµÐ»Ð¸
Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸ÑÑ Ð´Ð¸Ð¾ÐºÑÐ¸Ð´Ð° ÐºÑÐµÐ¼Ð½Ð¸Ñ
Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ñ
ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½Ð°Ñ Ð²Ð¸Ð·ÑÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ñ
nanobiotechnologies
polymeric microparticles
silica nanoparticles
modification
fluorescent visualization

description: Ð Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ñ Ð¸ Ð¿ÑÐ¾Ð²ÐµÑÐµÐ½Ñ ÑÐ¸ÑÑÐµÐ¼Ñ Ð½Ð°Ð½Ð¾- Ð¸ Ð¼Ð¸ÐºÑÐ¾ÑÐ°ÑÑÐ¸Ñ, Ð¿ÑÐ¸Ð¼ÐµÐ½Ð¸Ð¼ÑÐµ Ð´Ð»Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½ÑÑ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð²Ð¸Ð·ÑÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸. ÐÐ»Ñ Ð´Ð¾ÑÑÐ¸Ð¶ÐµÐ½Ð¸Ñ ÑÐµÐ»Ð¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¸ Ð¸Ð·ÑÑÐµÐ½Ñ Ð¿Ð¾ÑÐ»ÐµÐ´Ð½Ð¸Ðµ ÑÐ°Ð±Ð¾ÑÑ ÑÐ¾ ÑÑÐ¾Ð¶Ð¸Ð¼ Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð¸-ÐµÐ¼, Ð²ÑÐ±ÑÐ°Ð½Ð¾ Ð½ÐµÑÐºÐ¾Ð»ÑÐºÐ¾ Ð´Ð¾ÑÑÑÐ¿Ð½ÑÑ Ð´Ð»Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½ÑÑ ÐºÑÐ°ÑÐ¸-ÑÐµÐ»ÐµÐ¹ Ð¸ Ð¿ÑÐ¾Ð¸Ð·Ð²ÐµÐ´ÐµÐ½Ð° Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐ°ÑÑÐ¸Ñ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ CaCO3. Ð¢Ð°ÐºÐ¶Ðµ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½ Ð¿ÑÐ¾ÑÐ¾ÐºÐ¾Ð» Ð¿Ð¾Ð²ÐµÑÑÐ½Ð¾ÑÑÐ½Ð¾Ð¹ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Ð¿ÑÐ¸Ð½ÑÐ¸Ð¿Ð¸Ð°Ð»ÑÐ½Ð¾ Ð½Ð¾Ð²ÑÑ Ð½Ð¾ÑÐ¸ÑÐµÐ»ÐµÐ¹ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ TiO2 Ð¸ ÑÐ¾Ð·Ð´Ð°Ð½Ñ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½ÑÐµ Ð½Ð°Ð½Ð¾Ð½Ð¾ÑÐ¸ÑÐµÐ»Ð¸, Ð¼ÐµÑÐµÐ½ÑÐµ Ð°Ð½ÑÐ¸ÑÐµÐ»Ð°Ð¼Ð¸. ÐÐ¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸ÑÐµÑÐºÐ¸Ð¼Ð¸ Ð¼ÐµÑÐ¾Ð´Ð°Ð¼Ð¸ Ð¸Ð·ÑÑÐµÐ½Ñ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ Ð¸ ÑÐ°Ð·Ð¼ÐµÑÐ½ÑÐµ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ ÑÐ°ÑÑÐ¸Ñ. ÐÐ»Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐ¾ÑÑÐ¾Ñ-ÑÐµÐ»ÑÐ½Ð¾ÑÑÐ¸ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð½ÑÑ Ð¼ÐµÑÐ¾Ð´Ð¸Ðº Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ñ in vitro Ð¸ in vivo ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÑ, Ð¿Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÑÑÐ¸Ðµ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½ÑÐµ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° Ð½Ð¾ÑÐ¸ÑÐµÐ»ÐµÐ¹. Ð ÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð¼ Ð¿ÑÐ¾Ð´ÐµÐ»Ð°Ð½-Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÐµÑÑÑ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ðµ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½ÑÑ ÑÐ°ÑÑÐ¸Ñ, Ð¼ÐµÑÐ¾Ð´Ð¸ÐºÐ¸ Ð¼Ð¾Ð´Ð¸ÑÐ¸-ÐºÐ°ÑÐ¸Ð¹ ÐºÐ¾ÑÐ¾ÑÑÑ Ð¿ÑÐ¸Ð¼ÐµÐ½Ð¸Ð¼Ñ Ð´Ð»Ñ ÑÐ¸ÑÐ¾ÐºÐ¾Ð³Ð¾ ÑÐ¿ÐµÐºÑÑÐ° Ð½Ð¾ÑÐ¸ÑÐµÐ»ÐµÐ¹. Ð¢Ð°ÐºÐ¶Ðµ Ð°Ð½Ð°Ð»Ð¸Ð· ÐºÐ¾Ð½ÐµÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¿ÑÐµÐ´Ð¾ÑÑÐ°Ð²Ð¸Ð» Ð¿ÑÐµÐ´Ð¿Ð¾ÑÑÐ»ÐºÐ¸ Ð´Ð»Ñ Ð¿Ð¾Ð½Ð¸Ð¼Ð°Ð½Ð¸Ñ Ð¿ÑÐ¾ÑÐµÑÑÐ¾Ð² Ð²Ð·Ð°Ð¸-Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ ÑÐ¾Ð·Ð´Ð°Ð½Ð½ÑÑ Ð½Ð¾ÑÐ¸ÑÐµÐ»ÐµÐ¹ Ñ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ð¼Ð¸ ÑÐ¸ÑÑÐµÐ¼Ð°Ð¼Ð¸, ÑÑÐ¾ ÑÐ²Ð»ÑÐµÑÑÑ Ð¾ÑÐ½Ð¾Ð²Ð¾Ð¹ Ð´Ð»Ñ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐ¸Ñ ÑÑÐ°Ð¿Ð¾Ð² ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ñ Ð¿ÑÐ¾ÐµÐºÑÐ°.
description: In this work, applicable for fluorescence imaging methods systems of nano- and microparticles are developed and tested. To achieve the goal of the research, recent works with a similar direction were studied, several fluorescent dyes avail-able for research were selected and the modification of microparticles based on CaCO3 was made. A protocol for surface modification of fundamentally new car-riers based on TiO2 has also been developed, and fluorescent nanocarriers labeled with antibodies have been created. To study the consistency of the developed techniques, in vitro and in vivo experiments were carried out to demonstrate the fluorescent properties of the carriers. The result of this work is the production of fluorescent particles, the modification techniques of which are applicable to a wide range of carriers. Also, the analysis of the final data provided the prerequi-sites for understanding the processes of interaction of the created carriers with biological systems, which is the basis for the subsequent stages of the project's development.