cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
HBP neuron morphology viewer
Creators: name: Rembrandt Bakker
name: Tiesinga, Paul
Publisher: name: F1000Research
Year: 2016

description: We introduce a web-based viewer that can read, display and manipulate neuron morphology files. The files can be ingested from local storage, or by using the built-in search filters that provide access to the HBP neuroinformatics platform [1] and the extensive NeuroMorpho.org repository [2]. The viewer is the first to process common neuron morphology files in the browser, using newly written javascript-based parsers for Neurolucida data files (both binary, ascii and xml versions), as well as the SWC format [3]. Once a file is selected, the contents of the file are displayed both as a hierarchical tree and an interactive 3D graphical rendering of the neuron, powered by the x3dom.js framework. The morphology can be rendered either as a set of lines (fast) or as a collection of connected cylinders with varying diameter (slower). The viewer is available online [4,5]. An important future goal is to use the data processing services contained in the viewer in automated workflows, to support use cases that arise from the human brain project [6].

Supported by the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement n° 604102 (HBP). Thanks to Neuronland.org for Neurolucida format specifications.

[1] https://nip.humanbrainproject.eu/
[2] http://neuromorpho.org
[3] Cannon RC, Turner DA, Pyapali GK, Wheal HV (1998). "An on-line archive of reconstructed hippocampal neurons." J Neurosci Methods. 84(1-2):49-54. doi: 10.1016/S0165-0270(98)00091-0.
[4] standalone version: https://neuroinformatics.nl/HBP/morphology-viewer/
[5] HBP collaboratory version: https://collab.humanbrainproject.eu/#/collab/47/nav/4835
[6] Tiesinga P, Bakker R, Hill S, Bjaalie JG (2015). "Feeding the human brain model." Curr Opin Neurobiol. 32:107-14. doi: 10.1016/j.conb.2015.02.003.
A major sporting event or an entertainment show? A content analysis of Australian television coverage of the 2016 Olympic and Paralympic Games
Creators: name: Rees, Leanne
name: Robinson, Priscilla
name: Shields, Nora
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Outside of the Paralympic Games, elite athletes with disability rarely feature in the media. Using mixed method content analysis, the aim of this study was to compare the production and content of the 2016 Paralympic and Olympic Games broadcasts of an Australian commercial television network. Data were collected from recordings of a daily highlights show of the Paralympic and Olympic Games using a data abstraction tool with a priori codes. Data were coded for–content, participant(s) and context. Results suggest the Paralympic Games was broadcast as an entertainment show rather than a major sporting event. There were greater attempts during the Paralympic broadcast to elicit emotion; notions of competition vs. participation were raised; and stories of disability ran parallel to those of hardship. These differences in media portrayal of elite athletes with disability feed into existing notions that under-values their athleticism.
Molecular characterization of the first saltwater crocodilepox virus genome sequences from the world’s largest living member of the Crocodylia
Creators: name: Sarker, Subir
name: Isberg, Sally R
name: Milic, Natalie L
name: Lock, Peter
name: Helbig, Karla
Publisher: name: La Trobe
Year: 2020
Subjects: Uncategorized

description: © 2018 The Author(s). Crocodilepox virus is a large dsDNA virus belonging to the genus Crocodylidpoxvirus, which infects a wide range of host species in the order Crocodylia worldwide. Here, we present genome sequences for a novel saltwater crocodilepox virus, with two subtypes (SwCRV-1 and -2), isolated from the Australian saltwater crocodile. Affected belly skins of juvenile saltwater crocodiles were used to sequence complete viral genomes, and perform electron microscopic analysis that visualized immature and mature virions. Analysis of the SwCRV genomes showed a high degree of sequence similarity to CRV (84.53% and 83.70%, respectively), with the novel SwCRV-1 and -2 complete genome sequences missing 5 and 6 genes respectively when compared to CRV, but containing 45 and 44 predicted unique genes. Similar to CRV, SwCRV also lacks the genes involved in virulence and host range, however, considering the presence of numerous hypothetical and or unique genes in the SwCRV genomes, it is completely reasonable that the genes encoding these functions are present but not recognized. Phylogenetic analysis suggested a monophyletic relationship between SwCRV and CRV, however, SwCRV is quite distinct from other chordopoxvirus genomes. These are the first SwCRV complete genome sequences isolated from saltwater crocodile skin lesions.
Well defined difluorogold( iii ) complexes supported by N-ligands
Creators: name: Bayer, Mohammad Al
name: Corbo, Robert
name: Dutton, Jason
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Stable difluorogold(iii) complexes can be easily synthesized either via oxidation of N-ligated cationic Au(i) precursors using XeF or from tricationic Au(iii) precursors by displacement of the N-ligands using fluoride from economical KF. X-ray crystallographic studies of the bisimidazole ligated derivative shows the shortest Au-F bond known in a gold complex. 2
The impact of nurse and care staff education on the functional ability and quality of life of people living with dementia in aged care: A systematic review
Creators: name: Bauer, Michael
name: Fetherstonhaugh, Deirdre
name: Haesler, Emily
name: Beattie, Elizabeth
name: Hill, Keith D
name: Poulos, Christopher J
Publisher: name: La Trobe
Year: 2022
Subjects: classificationCode: 3901
subject: Curriculum and pedagogy
classificationCode: 42
subject: Health sciences
subject: FOS: Health sciences
classificationCode: 4203
subject: Health services and systems
classificationCode: 4204
subject: Midwifery
classificationCode: 4205
subject: Nursing

description: Objectives: Educational initiatives can improve care delivery and the experience of living in residential aged care for people with dementia. This review aimed to determine the impact of nurse and care staff education on measures of functional ability and quality of life for older people with dementia living in care homes. Design: Systematic review. Data sources: Search of on-line databases in English between January 2000 and January 2017. Review methods: Three reviewers used data extraction and critical appraisal tools of the Joanna Briggs Institute to determine methodological quality of research. Results and conclusion: Thirty-two studies met the initial inclusion criteria and 13 were retrieved for full appraisal. There was limited impact of nurse and care staff education on residents' agitation, anxiety, mood and quality of life. The most consistent improvement in functional ability due to education may be in the execution of activities of daily living. More successful programs included multi-faceted components (e.g. hands on support, clinical auditing) in conjunction with didactic teaching. There is a need for more rigorous and well-designed studies to test interventions.
LILACS Plus: desarrollo de la interfaz integrada de búsqueda
Creators: name: mendes abdala, carmen veronica
name: Yano Suga, Sueli Mitiko
name: Pinto, Rosemeire
Publisher: name: Zenodo
Year: 2024
Subjects: health information
latinamerica
lilacs
caribe
search interface

description: Presentación del concepto de la colección e interfaz de búsqueda LILACS Plus para la red de cooperación LILACS y BVS.
Esiste un universo nel quale siamo ciò che è e non ciò che vogliamo?
Creators: name: PsykoSapiens
Publisher: name: Zenodo
Year: 2024
Subjects: subject: Educational sciences
subject: FOS: Educational sciences
subject: Philosophy
subject: FOS: Philosophy, ethics and religion
subject: Psychology
subject: FOS: Psychology
subject: Sociology
subject: FOS: Sociology
subject: Social sciences
subject: FOS: Social sciences
subject: Social psychology
subject: Social issues
subject: Mental processes

description: L'avventura scientifica prosegue assumendo tutti i tratti di una profonda auto indagine psicologica e speculativa. Con un approccio che abbraccia il pensiero critico e l'osservazione della mente, esploriamo la natura della memoria, della reminiscenza e delle dinamiche nascoste del cosiddetto sé, sfidando i paradigmi convenzionali sulla psiche umana. Il cuore emerge come centro pulsante di saggezza ancestrale e "primo cervello", mentre il cervello è da noi visto come filtro funzionale per la realtà percepita.

Introduciamo due percorsi: il primo decostruisce l'idea comune di mente, mentre il secondo analizza il concetto di stortura mentale, evidenziando come la società contemporanea spesso promuova o ignori personalità disfunzionali e pericolose, quali il cosiddetto Narcisismo Maligno Perverso. Si badi, appositivi grammaticali sono e rimangono appositivi grammaticali, determinativi sono e rimangono determinativi. La nostra ricerca utilizza gli utilizzabili ma tenta di perforare le profondità che il linguaggio non può comprendere, e che lo stesso non può esprimere, ed esprimersi attraverso l'ente. In un'atmosfera di tensione tra controllo e perdita di virtù, colleghiamo il tema della degenerazione morale e il distacco umano dall'essenza, non apprezzando matrici virtuose o moraleggianti che fanno capo a illusioni.

Con uno sguardo scettico e quasi provocatorio, riconoscendo vi possa essere generazion di critica sul sociale di interpretazioni che muoverebbero a giudizi analitici, invitiamo a una riflessione sulle "forme umane" che, intrappolate in un infermo quotidiano, devono scegliere tra l'accoglimento passivo o la ricerca di verità in ciò che resiste a tale inferno.
Recovery after psychosis: qualitative study of service user experiences of lived experience videos on a recovery-oriented website
Creators: name: Williams, Anne
name: Fossey, Ellie
name: Farhall, John
name: Foley, Fiona
name: Thomas, Neil
Publisher: name: La Trobe
Year: 2023
Subjects: classificationCode: 42
subject: Health sciences
subject: FOS: Health sciences
classificationCode: 4201
subject: Allied health and rehabilitation science
classificationCode: 4203
subject: Health services and systems
classificationCode: 4205
subject: Nursing
classificationCode: 5203
subject: Clinical and health psychology

description: Background: Digital interventions offer an innovative way to make the experiences of people living with mental illness available to others. As part of the Self-Management And Recovery Technology (SMART) research program on the use of digital resources in mental health services, an interactive website was developed including videos of people with lived experience of mental illness discussing their recovery. These peer videos were designed to be watched on a tablet device with a mental health worker, or independently. Objective: Our aim was to explore how service users experienced viewing the lived experience videos on this interactive website, as well as its influence on their recovery journey. Methods: In total, 36 service users with experience of using the website participated in individual semistructured qualitative interviews. All participants had experience of psychosis. Data analysis occurred alongside data collection, following principles of constructivist grounded theory methodology. Results: According to participants, engaging with lived experience videos was a pivotal experience of using the website. Participants engaged with peers through choosing and watching the videos and reflecting on their own experience in discussions that opened up with a mental health worker. Benefits of seeing others talking about their experience included "being inspired," "knowing I'm not alone," and "believing recovery is possible." Experiences of watching the videos were influenced by the participants' intrapersonal context, particularly their ways of coping with life and use of technology. The interpersonal context of watching the videos with a worker, who guided website use and facilitated reflection, enriched the experience. Conclusions: Engaging with lived experience videos was powerful for participants, contributing to their feeling connected and hopeful. Making websites with lived experience video content available to service users and mental health workers demonstrates strong potential to support service users' recovery.
DESIGNING THE LEAST-COST MINI-GRID SOLUTION FOR THE LALA COMMUNITY
Creators: name: Azindow, Caleb
Publisher: name: Zenodo
Year: 2025
Subjects: Mini-Grid
Island Communities
Rural Electrification
Microgridspy
RAMP

description: My case study focuses on the technical and economic analysis to identify the most cost-effective mini-grid configuration to meet the energy needs of the Lala community. It outlines the community’s electricity demand (using the RAMP tool), assesses available renewable energy resources and evaluates system components such as solar PV, battery storage and diesel backup. Using modeling tools like MicroGridsPy, the report compares different system designs and determines the optimal mix that minimizes the Levelized Cost of Electricity (LCOE) while ensuring reliability and sustainability. The final recommendation balances affordability and scalability.
Maternal height and risk of caesarean section in singleton births in Sweden—A population-based study using data from the Swedish Pregnancy Register 2011 to 2016
Creators: name: Mogren, Ingrid
name: Lindqvist, Maria
name: Petersson, Kerstin
name: Nilses, Carin
name: Small, Rhonda
name: Granåsen, Gabriel
name: Edvardsson, Kristina
Publisher: name: La Trobe
Year: 2023
Subjects: classificationCode: 32
subject: Biomedical and clinical sciences
classificationCode: 3215
subject: Reproductive medicine
classificationCode: 42
subject: Health sciences
subject: FOS: Health sciences
classificationCode: 4204
subject: Midwifery

description: Caesarean section (CS) has short and long term adverse health consequences, and should therefore only be undertaken when necessary. Risk factors such as maternal age, maternal body mass index (BMI) and fetal weight have been extensively investigated in relation to CS, but the significance of maternal height has been less explored in Sweden. The aim was to investigate the significance of maternal height on risk of CS in a representative, population-based sample from Sweden, also taking into account confounders. Data on singleton births in the Swedish Pregnancy Register 2011 to 2016 were collected, including women with heights of 140 cm and above, constituting a sample of 581,844 women. Data were ana-lysed with epidemiological and biostatistical methods. Mean height was 166.1 cm. Women born outside Sweden were significantly shorter than women born in Sweden (162.8 cm vs. 167.1 cm, p<0.001). There was a decreasing risk of CS with increasing maternal height. This effect remained after adjustment for other risk factors for CS such as maternal age, BMI, gestational age, parity, high birth weight and country of birth. Frequency of CS was higher among women born outside Sweden compared with Swedish-born women (17.3% vs. 16.0%), however, in a multiple regression model country of birth outside Sweden diminished as a risk factor for CS. Maternal height of 178–179 cm was associated with the lowest risk of CS (OR = 0.76, CI95% 0.71–0.81), whereas height below 160 cm explained 7% of CS cases. BMI and maternal age are established factors involved in clinical assessments related to birth, and maternal height should increasingly enjoy a similar status in these considerations. Moreover, when healthcare professionals are counselling pregnant women, taller stature should be more emphasized as a positive indicator for successful vaginal birth to increase pregnant women’s confidence in giving birth vaginally, with possible positive impacts for lowering CS rates.
Lgl reduces endosomal vesicle acidification and Notch signaling by promoting the interaction between Vap33 and the V-ATPase complex
Creators: name: Portela-Esteban, Marta
name: Yang, Liu
name: Paul, Sayantanee
name: Li, Xia
name: Veraksa, Alexey
name: Parsons, Linda M
name: Richardson, Helena
Publisher: name: La Trobe
Year: 2022
Subjects: classificationCode: 31
subject: Biological sciences
subject: FOS: Biological sciences
classificationCode: 3101
subject: Biochemistry and cell biology

description: Epithelial cell polarity is linked to the control of tissue growth and tumorigenesis. The tumor suppressor and cell polarity protein lethal-2-giant larvae (Lgl) promotes Hippo signaling and inhibits Notch signaling to restrict tissue growth in Drosophila melanogaster. Notch signaling is greater in lgl mutant tissue than in wild-type tissue because of increased acidification of endosomal vesicles, which promotes the proteolytic processing and activation of Notch by γ-secretase. We showed that the increased Notch signaling and tissue growth defects of lgl mutant tissue depended on endosomal vesicle acidification mediated by the vacuolar adenosine triphosphatase (V-ATPase). Lgl promoted the activity of the V-ATPase by interacting with Vap33 (VAMP-associated protein of 33 kDa). Vap33 physically and genetically interacted with Lgl and V-ATPase subunits and repressed V-ATPase-mediated endosomal vesicle acidification and Notch signaling. Vap33 overexpression reduced the abundance of the V-ATPase component Vha44, whereas Lgl knockdown reduced the binding of Vap33 to the V-ATPase component Vha68-3. Our data indicate that Lgl promotes the binding of Vap33 to the V-ATPase, thus inhibiting V-ATPase-mediated endosomal vesicle acidification and thereby reducing γ-secretase activity, Notch signaling, and tissue growth. Our findings implicate the deregulation of Vap33 and V-ATPase activity in polarity-impaired epithelial cancers.
Lgl reduces endosomal vesicle acidification and Notch signaling by promoting the interaction between Vap33 and the V-ATPase complex
Creators: name: Portela-Esteban, Marta
name: Yang, Liu
name: Paul, Sayantanee
name: Li, Xia
name: Veraksa, Alexey
name: Parsons, Linda M
name: Richardson, Helena
Publisher: name: La Trobe
Year: 2022

description: Epithelial cell polarity is linked to the control of tissue growth and tumorigenesis. The tumor suppressor and cell polarity protein lethal-2-giant larvae (Lgl) promotes Hippo signaling and inhibits Notch signaling to restrict tissue growth in Drosophila melanogaster. Notch signaling is greater in lgl mutant tissue than in wild-type tissue because of increased acidification of endosomal vesicles, which promotes the proteolytic processing and activation of Notch by γ-secretase. We showed that the increased Notch signaling and tissue growth defects of lgl mutant tissue depended on endosomal vesicle acidification mediated by the vacuolar adenosine triphosphatase (V-ATPase). Lgl promoted the activity of the V-ATPase by interacting with Vap33 (VAMP-associated protein of 33 kDa). Vap33 physically and genetically interacted with Lgl and V-ATPase subunits and repressed V-ATPase-mediated endosomal vesicle acidification and Notch signaling. Vap33 overexpression reduced the abundance of the V-ATPase component Vha44, whereas Lgl knockdown reduced the binding of Vap33 to the V-ATPase component Vha68-3. Our data indicate that Lgl promotes the binding of Vap33 to the V-ATPase, thus inhibiting V-ATPase-mediated endosomal vesicle acidification and thereby reducing γ-secretase activity, Notch signaling, and tissue growth. Our findings implicate the deregulation of Vap33 and V-ATPase activity in polarity-impaired epithelial cancers.
PrECast: an efficient crypto-free solution for broadcast-based attacks in IPv4 networks
Creators: name: Hanna, Dalal
name: Veeraraghavan, Prakash
name: Pardede, Eric
Publisher: name: La Trobe
Year: 2023
Subjects: classificationCode: 40
subject: Engineering
classificationCode: 4009
subject: Electronics, sensors and digital hardware

description: Broadcasting is one of the essential features in the Internet Protocol Ver 4 (IPv4). Attackers often exploit this feature of the IP protocol to launch several attacks against a network or an individual host. Attackers may either be a part of a Local Area Network (LAN) or outside a LAN to launch these attacks. There are numerous papers available in the literature to solve problems resulting from IP broadcasting. However, all these solutions target a specific problem that results from IP broadcasting. Furthermore, these solutions use either a computationally-intensive cryptographic scheme, the a priori relation between the host and the network or a modified protocol stack at every host. In this paper, we provide a seamless and transparent solution to eliminate IP broadcasting and thus eliminate all problems related to IP broadcasting. Our proposed solution is crypto-free and does not need any modification to the protocol stack.
Prevalence of knee osteoarthritis features on magnetic resonance imaging in asymptomatic uninjured adults: a systematic review and meta-analysis
Creators: name: Culvenor, Adam
name: Øiestad, Britt Elin
name: Hart, Harvi
name: Stefanik, Joshua J
name: Guermazi, Ali
name: Crossley, Kay
Publisher: name: La Trobe
Year: 2022
Subjects: classificationCode: 32
subject: Biomedical and clinical sciences
classificationCode: 3202
subject: Clinical sciences
classificationCode: 4207
subject: Sports science and exercise
classificationCode: 5201
subject: Applied and developmental psychology

description: BACKGROUND: Knee MRI is increasingly used to inform clinical management. Features associated with osteoarthritis are often present in asymptomatic uninjured knees; however, the estimated prevalence varies substantially between studies. We performed a systematic review with meta-analysis to provide summary estimates of the prevalence of MRI features of osteoarthritis in asymptomatic uninjured knees. METHODS: We searched six electronic databases for studies reporting MRI osteoarthritis feature prevalence (ie, cartilage defects, meniscal tears, bone marrow lesions and osteophytes) in asymptomatic uninjured knees. Summary estimates were calculated using random-effects meta-analysis (and stratified by mean age: <40 vs ≥40 years). Meta-regression explored heterogeneity. RESULTS: We included 63 studies (5397 knees of 4751 adults). The overall pooled prevalence of cartilage defects was 24% (95% CI 15% to 34%) and meniscal tears was 10% (7% to 13%), with significantly higher prevalence with age: cartilage defect <40 years 11% (6%to 17%) and ≥40 years 43% (29% to 57%); meniscal tear <40 years 4% (2% to 7%) and ≥40 years 19% (13% to 26%). The overall pooled estimate of bone marrow lesions and osteophytes was 18% (12% to 24%) and 25% (14% to 38%), respectively, with prevalence of osteophytes (but not bone marrow lesions) increasing with age. Significant associations were found between prevalence estimates and MRI sequences used, physical activity, radiographic osteoarthritis and risk of bias. CONCLUSIONS: Summary estimates of MRI osteoarthritis feature prevalence among asymptomatic uninjured knees were 4%-14% in adults aged <40 years to 19%-43% in adults ≥40 years. These imaging findings should be interpreted in the context of clinical presentations and considered in clinical decision-making.
Er ger man zo eun den yaouank
Creators: name: Musée National Des Arts Et Traditions Populaires (Paris)
name: Centre D'ethnologie Française
Publisher: name: École des Hautes Études en Sciences Sociales; Institut  interdisciplinaire d'anthropologie du contemporain (Paris); Laboratoire d'anthropologie et d'histoire de l'institution de la culture (Paris)
Year: 2024

Abstract
The Influence of Photoelectron Escape in Radiation Damage Simulations of Protein Micro-Crystallography
Creators: name: Marman, Hugh
name: Darmanin, Connie
name: Abbey, Brian
Publisher: name: La Trobe
Year: 2023
Subjects: classificationCode: 34
subject: Chemical sciences
subject: FOS: Chemical sciences
classificationCode: 3402
subject: Inorganic chemistry

description: Radiation damage represents a fundamental limit in the determination of protein structures via macromolecular crystallography (MX) at third-generation synchrotron sources. Over the past decade, improvements in both source and detector technology have led to MX experiments being performed with smaller and smaller crystals (on the order of a few microns), often using microfocus beams. Under these conditions, photoelectrons (PEs), the primary agents of radiation-damage in MX, may escape the diffraction volume prior to depositing all of their energy. The impact of PE escape is more significant at higher beam energies (>20 keV) as the electron inelastic mean free path (IMFP) is longer, allowing the electrons to deposit their energy over a larger area, extending further from their point of origin. Software such as RADDOSE-3D has been used extensively to predict the dose (energy absorbed per unit mass) that a crystal will absorb under a given set of experimental parameters and is an important component in planning a successful MX experiment. At the time this study was undertaken, dose predictions made using RADDOSE-3D were spatially-resolved, but did not yet account for the propagation of PEs through the diffraction volume. Hence, in the case of microfocus crystallography, it is anticipated that deviations may occur between the predicted and actual dose absorbed due to the influence of PEs. To explore this effect, we conducted a series of simulations of the dose absorbed by micron-sized crystals during microfocus MX experiments. Our simulations spanned beam and crystal sizes ranging from 1 µm to 5 µm for beam energies between 9 keV and 30 keV. Our simulations were spatially and temporarily resolved and accounted for the escape of PEs from the diffraction volume. The spatially-resolved dose maps produced by these simulations were used to predict the rate of intensity loss in a Bragg spot, a key metric for tracking global radiation damage. Our results were compared to predictions obtained using a recent version of RADDOSE-3D that did not account for PE escape; the predicted crystal lifetimes are shown to differ significantly for the smallest crystals and for high-energy beams, when PE escape is included in the simulations.
title: Interim Report (Berlin Festival '51)
Creators: name: Lettunich, Mateo
Publisher: name: George Mason University
Year: 1995
Subjects: subject: Berlin Festival
subject: ANTA
subject: Caldwell, Oliver
subject: Breen, Robert
subject: Varnay, Astrid
subject: Kapell, William
subject: Traubel, Helen

description: Report.
Enrichment of extracellular vesicles from human synovial fluid using size exclusion chromatography
Creators: name: Foers, Andrew D
name: Chatfield, Simon
name: Dagley, Laura F
name: Scicluna, Benjamin J
name: Webb, Andrew I
name: Sim, Lesley
name: Hill, Andrew
name: Wicks, Ian P
name: Pang, Ken C
Publisher: name: La Trobe
Year: 2023
Subjects: classificationCode: 31
subject: Biological sciences
subject: FOS: Biological sciences
classificationCode: 3101
subject: Biochemistry and cell biology

description: As a complex biological fluid, human synovial fluid (SF) presents challenges for extracellular vesicle (EV) enrichment using standard methods. In this study of human SF, a size exclusion chromatography (SEC)-based method of EV enrichment is shown to deplete contaminants that remain after standard ultracentrifugation-based enrichment methods. Specifically, considerable levels of serum albumin, the high-density lipoprotein marker, apolipoprotein A-I, fibronectin and other extracellular proteins and debris are present in EVs prepared by differential ultracentrifugation. While the addition of a sucrose density gradient purification step improved purification quality, some contamination remained. In contrast, using a SEC-based approach, SF EVs were efficiently separated from serum albumin, apolipoprotein A-I and additional contaminating proteins that co-purified with high-speed centrifugation. Finally, using high-resolution mass spectrometry analysis, we found that residual contaminants which remain after SEC, such as fibronectin and other extracellular proteins, can be successfully depleted by proteinase K. Taken together, our results highlight the limitations of ultracentrifugation-based methods of EV isolation from complex biological fluids and suggest that SEC can be used to obtain higher purity EV samples. In this way, SEC-based methods are likely to be useful for identifying EV-enriched components and improving understanding of EV function in disease.
Consapevolezza
Creators: name: PsykoSapiens
Publisher: name: Zenodo
Year: 2024
Subjects: subject: Educational sciences
subject: FOS: Educational sciences
subject: Philosophy
subject: FOS: Philosophy, ethics and religion
subject: Psychology
subject: FOS: Psychology
subject: Sociology
subject: FOS: Sociology
subject: Social issues
subject: Social psychology
subject: Social sciences
subject: FOS: Social sciences
subject: Mental processes

description: Il taccuino esplora una concezione profonda e critica della mente, contrapponendola alla visione comune del cervello come centro dell'intelletto e della percezione.

La mente, noi sosteniamo, non risiede all'interno del cervello né è limitata alle sue attività neuronali, ma è piuttosto guidata dal cuore, organo che infonde al pensiero non pensato una direzione - al sociale senso - profonda e vitale attraverso il thumos, che plasma l'interazione con l'intimo mondo.

La vulnerabilità della mente alle influenze esterne al corpo è data dalla "educazione" pavloviana, che modella il pensiero pensato fino all'incapacità della contemporaneità di cogliere la cruda realtà dell'esistenza e dell'esistere. Si configurano - a tal guisa - illusioni, concretizzate nell'incarnato di persone, maschere cadute nel mondo, che vivono all'interno di una gabbia, senza mai affrontare la verità autentica del loro essere.

Le tragedie dell'esistenza sono divenute drammi e disgrazie: lo scontro fra l'esperienza vissuta e il condizionamento mentale crea uno iato, una frattura tra l'essere e il nulla, che trasforma l'individuo in un partecipante inconsapevole della cosiddetta "vita".

Meraviglia. La banalità del male e la stupidità sono impedimenti alla vera libertà e consapevolezza. Solo lo sguardo innocente e spontaneo del fanciullo è capace di accedere alla "vita vera", mentre l'individuo già liquido si perde nelle sovrastrutture artatamente sovrastrutturate della società e dell'apparenza, mosso dallo sguardo della degenerazione della cultura odierna.
Mitochondrial HTRA2 Plays a Positive, Protective Role in Dictyostelium discoideum but Is Cytotoxic When Overexpressed
Creators: name: Chen, Suwei
name: Sanislav, Oana
name: Annesley, Sarah
name: Fisher, Paul
Publisher: name: La Trobe
Year: 2023
Subjects: classificationCode: 31
subject: Biological sciences
subject: FOS: Biological sciences
classificationCode: 3101
subject: Biochemistry and cell biology
classificationCode: 3105
subject: Genetics

description: HTRA2 is a mitochondrial protein, mutations in which are associated with autosomal dominant late-onset Parkinson’s disease (PD). The mechanisms by which HTRA2 mutations result in PD are poorly understood. HTRA2 is proposed to play a proteolytic role in protein quality control and homeostasis in the mitochondrial intermembrane space. Its loss has been reported to result in accumulation of unfolded and misfolded proteins. However, in at least one case, PD-associated HTRA2 mutation can cause its hyperphosphorylation, possibly resulting in protease hyperactivity. The consequences of overactive mitochondrial HTRA2 are not clear. Dictyostelium discoideum provides a well-established model for studying mitochondrial dysfunction, such as has been implicated in the pathology of PD. We identified a single homologue of human HTRA2 encoded in the Dictyostelium discoideum genome and showed that it is localized to the mitochondria where it plays a cytoprotective role. Knockdown of HTRA2 expression caused defective morphogenesis in the multicellular phases of the Dictyostelium life cycle. In vegetative cells, it did not impair mitochondrial respiration but nonetheless caused slow growth (particularly when the cells were utilizing a bacterial food source), unaccompanied by significant defects in the requisite endocytic pathways. Despite its protective roles, we could not ectopically overexpress wild type HTRA2, suggesting that mitochondrial HTRA2 hyperactivity is lethal. This toxicity was abolished by replacing the essential catalytic serine S300 with alanine to ablate serine protease activity. Overexpression of protease-dead HTRA2 phenocopied the effects of knockdown, suggesting that the mutant protein competitively inhibits interactions between wild type HTRA2 and its binding partners. Our results show that cytopathological dysfunction can be caused either by too little or too much HTRA2 activity in the mitochondria and suggest that either could be a cause of PD.
Enhancer-gene networks for the identification of cancer driver genes affected by enhancer mutations
Creators: name: Kariotis, Sokratis
name: Ridder, Jeroen De
name: Huisman, Sjoerd
Publisher: name: F1000Research
Year: 2016

description: Dynamic and diverse epigenetic modifications on enhancers affect the expression of target genes through DNA looping. Aberrant epigenetic modifications on these regions may result in misregulated gene expression. As deregulated gene expression is one of the important hallmarks of cancer, the study of such genomic regulatory elements is an important field of study in cancer research. As a step towards identifying enhancers with a potential driving role in cancer, we have constructed a enhancer-gene (EG) network by pairing the recently defined enhancer regions with targeted genes based on the correlation between epigenetic mark enrichment and gene expression across a wide range of cell types. The constitutive pairings are subsequently validated in silico using Hi-C measurements that capture the 3D conformation of the chromosomes. The EG-networks are overlaid with known cancer genes and noncoding somatic variation obtained from whole cancer genome sequencing. These networks enable identification of enriched modules that point to cancer drivers that are affected through somatic variations in the non coding genome.
Studying phenotypic impact of non-synonymous single nucleotide variants in Oryza sativa
Creators: name: Sayane Shome
name: Meena, Rakesh Kumar
Publisher: name: F1000Research
Year: 2016

description: Understanding the impact of genomic variant on protein functions is crucial to determine if their presence supports or curbs the protein expression. In the current study, we are focussing on two key proteins with roles in signalling pathways linked with oligosaccharide processing and induction of disease, stress resistance respectively. One of them, endo-βN acetylglucosaminidase is an enzyme exhibiting hydrolase activity and playing a key role in processing of oligosaccharides in the cytosol. Whereas WRKY45 is a transcription factor involved in resistance to pathogen infection in multiple species. The genes encoding these enzymes are located in LOC_Os05g27960  and LOC_Os05g25570  respectively, in rice (Oryza sativa japonica). A computational workflow is devised to identify deleterious SNPs and determine how they affect the functional mechanisms of translated protein products. The inferences drawn from the study are useful to determine variants which can targeted for experimental studies as well as provided insights on mechanisms as how deleterious variants are deterring the protein function.
Supplementary document for Highly polarized light emission from hybrid of quantum dots and plasmons in the intermediate coupling regime - 6421925.pdf
Creators: name: Lan, Xinhui
name: Shi, Xiuqi
name: Zhang, Lan
name: Guo, Qianqian
name: Zou, Zhen
name: Zhu, Guangpeng
name: Li, Xiang
name: Du, Wei
name: Wang, Tao
Publisher: name: Optica Publishing Group
Year: 2025
Subjects: Uncategorized

description: Supplement 1
Al labousig er c'hoad
Creators: name: Musée National Des Arts Et Traditions Populaires (Paris)
name: Centre D'ethnologie Française
Publisher: name: École des Hautes Études en Sciences Sociales; Institut  interdisciplinaire d'anthropologie du contemporain (Paris); Laboratoire d'anthropologie et d'histoire de l'institution de la culture (Paris)
Year: 2024

Abstract
Temporal Associations of Alcohol and Tobacco Consumption With Cancer Mortality
Creators: name: Jiang, Heng
name: Livingston, Michael
name: Room, Robin
name: Chenhall, Richard
name: English, Dallas R
Publisher: name: La Trobe
Year: 2023
Subjects: classificationCode: 32
subject: Biomedical and clinical sciences
classificationCode: 42
subject: Health sciences
subject: FOS: Health sciences
classificationCode: 4203
subject: Health services and systems
classificationCode: 4206
subject: Public health

description: Importance: Understanding whether the population-level consumption of alcohol and tobacco is associated with cancer mortality is a crucial question for public health policy that has not been answered by previous studies. Objective: To examine temporal associations of alcohol and tobacco consumption with overall cancer mortality in the Australian population, looking across different sex and age groups. Design, Setting, and Participants: This population-based cohort study conducted a time series analysis (autoregressive integrated moving average models) using aggregate-level annual time series data from multiple sources. Data on alcohol consumption and tobacco consumption per capita between 1935 and 2014 among the Australian population aged 15 years and older were collected from the Australian Bureau of Statistics and Cancer Council Victoria. Analysis was conducted from June 1, 2017, to October 30, 2017. Exposures: Sex- and age-specific cancer mortality rates from 1968 to 2014 were collected from the Australian Institute Health and Welfare. Main Outcomes and Measures: Population-level cancer mortality in different sex and age groups in Australia, controlling for the effects of health expenditure. Results: Among the Australian total population aged 15 years and older in this study, 50.5% were women. Cancer death rates per 100 000 persons increased from 199 in 1968 to 214 in 1989 and then decreased steadily to 162 in 2014. Taking into account lagged effects, 1-L decreases in alcohol consumption per capita were associated with a decline of 3.9% in overall cancer mortality over a 20-year period, and 1-kg decreases in tobacco consumption per capita were associated with a 16% reduction. Alcohol consumption per capita was significantly associated with overall cancer mortality among men aged 50 to 69 years and women aged 50 years and older. Tobacco consumption per capita was found to be significantly associated with overall cancer mortality only among men aged 50 years and older. Conclusions and Relevance: In this study, alcohol consumption per capita was positively associated with overall cancer mortality among older men and women, and tobacco consumption per capita was positively associated with overall cancer mortality among older men over a 20-year period. This study provides evidence that a decrease in population-level drinking and tobacco smoking could lead to a reduction in cancer mortality.
Recchia Lane 1966
Creators: name: Dalens, Pierre-Henri
name: Robin, Frédéric
Publisher: name: Zenodo
Year: 2024
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Coleoptera
Cerambycidae
Recchia

description: Recchia Lane, 1966
- AErenica Thomson,1857: 311 (nec Dejean, 1835); 1860: 42, 65; 1864: 129, 403; Lacordaire, 1872: 898; Girard, 1873: 751; Bates, 1881: 206.
- Aerenica Zajciw & Ruffinelli, 1962: 84 (nec Dejean, 1835); Gilmour, 1962: 126, 127; Viana, 1972: 400; Lane, 1974: 352.
Espèce - type: Saperda hirticornis Klug, 1825 par désignation de Thomson, 1864: 129.
- Recchia Lane, 1966: 232; Martins & Galileo, 1985: 481; Monné & Giesbert, 1992: 253; Galileo & Martins, 1992: 143 (synonymie); Monné, 1995: 67; Martins & Galileo, 1998: 57 (synonymie); Monné, 2005: 240; 2012: 82.
Espèce-type: Recchia ludibriosa Lane, 1966 par désignation originale & monotypie.
- Coruparana Lane, 1966: 233; Martins & Galileo, 1985: 468; Monné, 1995: 66.
Reviewers:
Gérard Tavakilian (MNHN - Muséum national d’Histoire naturelle, Paris, France). - http://zoobank.org/ 607BDB90-7FE1-42A6-9329-8982CE39E658 Espèce - type: Coruparana veruta Lane,1966 par désignation originale.
- Trichohippopsides Breuning, 1980: 67.
Espèce-type: Trichohippopsides albicans Breuning (= Saperda albicans Guérin-Méneville, 1831) par désignation originale & monotypie.
description: Published as part of Dalens, Pierre-Henri & Robin, Frédéric, 2024, Recchia hirticornis (Klug, 1825) et description d'une nouvelle espèce de Recchia Lane, 1966 de Guyane (Coleoptera: Cerambycidae, Lamiinae), pp. 1-7 in Faunitaxys 12 (10) on pages 1-2, DOI: 10.57800/faunitaxys-12(10), http://zenodo.org/record/15365906
title: Analyzing Hardware Based Malware Detectors Using Machine Learning Techniques
Creators: name: Randive, Onkar
Publisher: name: George Mason University
Year: 2019
Subjects: subject: Hardware performance counters
subject: Machine learning
subject: Malware detection
subject: Malware classification

description: Growth of malware has been a serious problem in the technology community and would continue to grow with new advances in technology. Traditional software-based malware detection systems have proved to be inadequate. Behavioral malware detection systems have proved to be an improvement but are limited due to the fact that they are resource intensive and still prone to exploitation. Hardware based malware detection has proved to be an effective answer to reduce exploitability of computer systems due to less visibility and access for exploitation. This work shows the results of using different machine learning classifiers for Hardware based malware detection. Further, it analyzes the hardware implementation of these machine classifiers on an FPGA by discussing the latency and area requirements of the machine learning classifiers. It is proposed that classifiers with less or limited number of features for reduced system overhead especially in resource constrained environments like real time systems or embedded systems. Hence, the number of features fed to the classifier are reduced by using feature selection technique called Principal Component Analysis. Simple ML Classifiers like JRIP and OneR prove to be more efficient than complex ML classifiers like neural networks. The results for multiclass classification using Multinomial logistic regression, Multilayer Perceptron and Support Vector machines show that PCA assisted multiclass classifies prove to be 7% more efficient than regular Multiclass classifiers. Further the limitations of the above work are discussed by explaining solution leading to future works in this topic.
Il Senso della Vita
Creators: name: PsykoSapiens
Publisher: name: Zenodo
Year: 2024
Subjects: subject: Philosophy
subject: FOS: Philosophy, ethics and religion
subject: Psychology
subject: FOS: Psychology
subject: Social psychology
subject: Social sciences
subject: FOS: Social sciences
subject: Educational sciences
subject: FOS: Educational sciences
subject: Social issues
subject: Sociology
subject: FOS: Sociology
subject: Mental processes

description: Il taccuino esplora la complessità della cosiddetta condizione umana all'interno di un'esistenza priva di senso, focalizzandosi sulla figura del cosiddetto narcisista maligno perverso. Esso, inadatto all'esistenza, alla vita sociale e relazionale, agisce come forza distruttiva, da forza distruttiva, in forza distruttiva. Avvelena e danneggia coloro con cui entra in contatto. La sua natura violenta e tossica porta a un deterioramento fisico e psichico sia di se stesso che degli altri, minacciando ogni forma d'armonia e benessere, libertà e felicità.

La malvagità e l'inganno del NMP conducono a una perdita di significato e a un senso di vuoto esistenziale. Sottolineeremo la necessità di comprensione, autoconsapevolezza e educazione per riconoscere e contrastare tali dinamiche distruttive. La psichiatria tradizionale, sosteniamo, non riesce a cogliere appieno queste complessità. Proponiamo un approccio olistico e individualizzato per valutare la tossicità delle interazioni umane.
Discriminating the earliest stages of mammary carcinoma using myoepithelial and proliferative markers
Creators: name: Duivenvoorden, Hendrika M
name: Spurling, Alex
name: O'Toole, Sandra A
name: Parker, Belinda
Publisher: name: La Trobe
Year: 2023
Subjects: classificationCode: 32
subject: Biomedical and clinical sciences
classificationCode: 3211
subject: Oncology and carcinogenesis

description: Mammographic screening has led to increased detection of breast cancer at a pre-invasive state, hence modelling the earliest stages of breast cancer invasion is important in defining candidate biomarkers to predict risk of relapse. Discrimination of pre-invasive from invasive lesions is critically important for such studies. Myoepithelial cells are the barrier between epithelial cells and the surrounding stroma in the breast ductal system. A number of myoepithelial immunohistochemistry markers have been identified and validated in human tissue for use by pathologists as diagnostic tools to distinguish in situ carcinoma from invasive breast cancer. However, robust myoepithelial markers for mouse mammary tissue have been largely under-utilised. Here, we investigated the utility of the myoepithelial markers smooth muscle actin (SMA), smooth muscle myosin heavy chain (SMMHC), cytokeratin-14 (CK14) and p63 to discriminate mammary intraepithelial neoplasia (MIN) from invasive disease in the C57BL/ 6J MMTV-PyMT transgenic model of mammary carcinoma. We identified that SMMHC and CK14 are retained in early in situ neoplasia and are appropriate markers for distinguishing MIN from invasive disease in this model. Additionally, the proliferation marker Ki67 is a superior marker for differentiating between normal and hyperplastic ducts, prior to the development of MIN. Based on this, we developed a scoring matrix for discriminating normal, hyperplasia, MIN and invasive lesions in this spontaneous mammary tumorigenesis model. This study demonstrates heterogeneous expression of myoepithelial proteins throughout tumour development, and highlights the need to characterise the most appropriate markers in other models of early breast cancer to allow accurate classification of disease state.
title: Newspaper Column: Culinary Virtuosity Invades Fairfax County
Creators: name: Nicoson, William J.
Publisher: name: Reston Times
Year: 2003
Subjects: subject: Haeringer, Francois
subject: Great Falls (VA)
subject: Cam, Yannick
subject: Tysons Corner (VA)
subject: Krimm, Jonathan
subject: Restaurants

description: PDF file distilled from original WordPerfect document. Original size 11" x 8.5".
description: In April, 1976, the famous chef, François Haeringer, having abandoned his downtown location on 15 Street near the Ellipse, opened L’Auberge Chez François in the wilds of Great Falls. He th was the first great chef to establish himself in an ever-urbanizing Fairfax County. His Auberge, now guided by his son, Jacques, made the best of Alsatian cuisine popular among those who respected careful and novel cooking as an art form.