title: ÐÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð¾ÑÐ½Ð¾Ð²Ð½ÑÑ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÐ¾Ð² ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð°Ð»ÑÐ½ÑÑ ÑÑÑÐ¾Ð¼Ð°Ð»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð½Ð° Ð¼Ð¸ÐºÑÐ¾Ð½Ð¾ÑÐ¸ÑÐµÐ»ÑÑ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Definition of the main parameters cultivation of human mesenchymal stromale cells on microcarriers
Creators: name: ÐÐ¾ÑÐ¸ÑÐµÐ²Ð°, ÐÐ½Ð°ÑÑÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÐÐ»ÐµÑÐºÐ° (Ð±Ð¸Ð¾Ð».)
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Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÑ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ
human mesenchymal stromal cells
placenta
microcarriers
cultivation
cultivation parameters

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description: The subject of the graduate qualification work is "Determination of the basic parameters of the cultivation of human mesenchymal stromal cells on microcarriers". This given work is devoted to the selection of the main parameters of the cultivation of human mesenchymal stromal cells of placental origin on microcarriers in a spinner. As part of the work, the following tasks were solved: - a literary analysis on the research topic was carried out and the main stages and key parameters of the cultivation of MSCs on MN were identified; - a series of experiments was conducted to establish optimal cultivation parameters; - the development of the target product in 3 repetitions and quality control of the developed MSCs were carried out in accordance with the requirements of the International Society of Cell Therapy. The paper presents the results of the study, on the basis of which the optimal values of the following parameters of MSCs cultivation on MC were determined: preparation of the seed crop, concentration of the seed crop, MC concentration, duration and mode of mixing at the attachment stage, composition of growth media at the attachment stage and at the stage of development of the target product, pseudoperforation mode, the mode of dissociation of MSC with MC. Confirmed the reproducibility of the process at the set parameters in 3 repetitions. The quality of the developed MSCs was confirmed in accordance with the requirements of the International Society for Cell Therapy.