title: Ð¡Ð¾Ð·Ð´Ð°Ð½Ð¸Ðµ ÑÐºÐ°Ð½ÐµÐ¸Ð½Ð¶ÐµÐ½ÐµÑÐ½ÑÑ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ð¹ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ ÑÐµÐ»Ð»ÑÐ»Ð¾Ð·Ð½ÑÑ Ð¼Ð°ÑÑÐ¸Ñ, Ð·Ð°ÑÐµÐ»ÐµÐ½Ð½ÑÑ Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð°Ð»ÑÐ½ÑÐ¼Ð¸ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÐ¼Ð¸ ÐºÐ»ÐµÑÐºÐ°Ð¼Ð¸ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Development of tissue engineering structures based on cellulose matrices populated with human mesenchymal stem cells
Creators: name: ÐÑÐ½ÐµÐµÐ², ÐÐ²Ð°Ð½
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: ÑÐºÐ°ÑÑÐ¾Ð»Ð´
Ð¼ÐµÐ·ÐµÐ½Ñ Ð¸Ð¼Ð°Ð»ÑÐ½ÑÐµ ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸
ÑÐºÐ°Ð½ÐµÐ¸Ð½Ð¶ÐµÐ½ÐµÑÐ½ÑÐµ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ð¸
scaffold
mesenchymal stem cells
tissue engineering constructions

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description: The development of the tissue-engineered structures based on the human stem cells is an actively evolving field of regenerative medicine. Decellularization of animal and plant tissues acts as a promising technique for obtaining optimal scaffolds for such structures.
The aim of the present study was to create tissue-engineered structures based on cellulose matrices made of apple hypanthium tissues and populated with human mesenchymal stem cells (MSCs), as well as their approbation for the treatment of wounds on the skin of experimental animals (mice).
Cellulose porous matrices were obtained by detergent decellularization using sodium dodecyl sulfate solution. To improve their adhesion properties to MSCs of endometrial origin (eMSCs), a technique for coating matrices with collagen was developed. It was found that eMSCs cultivated as a part of such 3D-systems under certain seeding conditions are prone to the formation of viable spheroids in the pores of the matrix. In comparison to conventional monolayer cultures, spheroids formed by eMSCs showed a significantly higher level of vascular endothelial growth factor secretion. Matrices obtained from apple tissue and populated with human eMSCs were tested as tissue-engineering constructs on the model of skin wounds in mice. Administration of scaffolds containing eMSC spheroids within the wound cavity led to the increased rate of its healing, reached presumably due to enhanced islet epithelialization.