Data for: The eco-evolutionary significance of rainfall constancy for facultative CAM photosynthesis
Creators: name: Schweiger, Andreas
name: Nürk, Nicolai
name: Beckett, Heath
name: Liede-Schumann, Sigrid
name: Midgley, Guy
name: Higgins, Steven
Publisher: name: figshare
Year: 2021
Subjects: subject: 60302 Biogeography and Phylogeography
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Botany
subject: 60203 Ecological Physiology
subject: 60399 Evolutionary Biology not elsewhere classified
subject: Plant Biology

description: <b>Article Summary</b><b><br></b>● A flexible use of the crassulacean acid metabolism (CAM) has been hypothesised to represent an intermediate stage along a C3 to full CAM evolutionary continuum, where relative contributions of C3 vs. CAM metabolism are co-determined by evolutionary history and prevailing environmental constraints. However, evidence for such eco-evolutionary interdependencies is lacking.<br>● We studied these interdependencies for the leaf-succulent genus Drosanthemum (Aizoaceae, Southern African Succulent Karoo) by testing for relationships between leaf δ13C diagnostic for CAM dependence (i.e. contribution of C3 and CAM to net carbon gain), and climatic variables related to temperature and precipitation and their temporal variation. We further quantified the effects of shared phylogenetic ancestry on CAM dependence and its relation to climate.<br>● CAM dependence is predicted by rainfall and its temporal variation, with high predictive power of rainfall constancy (temporal entropy). The predictive power of rainfall seasonality and temperature-related variables was negligible. Evolutionary history of the tested clades significantly affected the relationship between rainfall constancy and CAM dependence.<br>● We argue that higher CAM dependence might provide an adaptive advantage in increasingly unpredictable rainfall environments when the anatomic exaptation (succulence) is already present. These observations might shed light on the evolution of full CAM.<br><br><b>Files included in the repository:</b><br><b>Schweiger_etal_Drosanthemum_Analyses.R</b><br>- R script to perform the analyses and generate figures<br><b>Drosanthemum_13C_CRUclimate_20200728phySorted.csv<br></b><b>Drosanthemum_13C_CRUclimate_20200728phySorted.xlsx</b><br>- Climatic data used in the analyses. Data are from the Climatic Research Unit of East Anglia Database (CRU TS v. 4.03; Harris et al., 2020; retrieved from: https://crudata.uea.ac.uk/cru/data/hrg/cru_ts_4.03/)<br><b>Dros2_cp_all_n141Gb.phy</b><br>- Alignment file for partitioning and substitution model estimation in PartitionFinder 2 (Lanfear et al., 2016)<br><b>RAxML_bestTree.Dros2_cp_all_n141Gb.tre</b><br>- ML phylogeny file for partitioning and substitution model estimation in PartitionFinder 2 (Lanfear et al., 2016)<br><br><b>Dros2_cp1_tK16_n141Gb.nex,</b><b>Dros2_cp2_r16Q_n141Gb.nex,</b><b>Dros2_cp3_trnSG_n141Gb.nex,</b><b>Dros2_cp4_rpl16_n141Gb.nex</b>- Single marker alignment files for divergence time estimation in BEAST1<br><br><b>script_partitionFinder_Drosanthemum.txt</b><br>- Script to run the analyses in PartitionFinder2<br><b>partition_finder.cfg</b><br>- Configuration file for PartitionFinder2<br><b>best_scheme.txt,<br></b><b>partitions_Dros2_cp_all_n141_Gb.txt</b><br>- Results from PartitionFinder2<br><b>Dros2_cpAll_n141Gb_Beast1_D.log<br></b><b>Dros2_cpAll_n141Gb_Beast1_D.mle.log<br></b><b>Dros2_cpAll_n141Gb_Beast1_D.mle.RESULTS.txt<br></b><b>Dros2_cpAll_n141Gb_Beast1_D.xml</b><br>- BEAST xml input files for model M1 and results produced by BEAST (MCMC log &amp; marginal likelihood estimations)<br><b>Dros2_cpAll_n141Gb_Beast2_D.log<br></b><b>Dros2_cpAll_n141Gb_Beast2_D.mle.log<br></b><b>Dros2_cpAll_n141Gb_Beast2_D.mle.RESULTS.txt<br></b><b>Dros2_cpAll_n141Gb_Beast2_D.xml</b><br>- BEAST xml input files for model M2 and results produced by BEAST (MCMC log &amp; marginal likelihood estimations)<br><b>Dros2_cpAll_n141Gb_Beast3_D.log<br></b><b>Dros2_cpAll_n141Gb_Beast3_D.mle.log<br></b><b>Dros2_cpAll_n141Gb_Beast3_D.mle.RESULTS.txt<br></b><b>Dros2_cpAll_n141Gb_Beast3_D.xml</b><br>- BEAST xml input files for model M3 and results produced by BEAST (MCMC log &amp; marginal likelihood estimations)<br><b>Dros2_cpAll_n141Gb_Beast1_ABCD75b.conl.red.tre</b><br>- Maximum clade credibility trees (excluding OGs) from the BEAST analysis used in the comparative analysis<br><b>Dros2_cpAll_n141Gb_Beast1_ABCD75b.1000l.red.trees</b><br>- Random sample of the posterior of 1000 time-scaled trees (excluding OGs) from the BEAST analysis used in the comparative analysis<br><b>Dros2_cpAll_n141Gb_Beast1_ABCD_75b.con.tre</b><br>- Maximum clade credibility trees (including OGs) from the BEAST analysis used in the comparative analysis<br><br><b>Dros2_cpAll_n141Gb_Beast1_ABCD_75b.trees</b><br>- Posterior sample of trees used to generate "Dros2_cpAll_n141Gb_Beast1_ABCD_75b.con.tre"<br>