cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Bio-inspired pH-responsive polymers for intracellular delivery of macromolecules and cell engineering applications
Creators: name: Morrison, Gabriella Angelique
Publisher: name: Imperial College London
Year: 2022

description: Biological macromolecules including peptides, proteins and antibodies can act as therapeutic agents. When delivered intracellularly to their desired target site within the cell, they can modify cell behaviour. However, a long-standing challenge is the efficient delivery of such materials to their intracellular target sites. Although effective in some applications, existing technologies to improve delivery, namely viral vectors, have key limitations in their toxicity and safety. There is thus a need for more effective intracellular delivery tools. This thesis explores the use of bio-inspired pH-responsive polymers as intracellular delivery platforms for the enhanced uptake of macromolecules. PP50, an anionic polymer comprised of a poly-L-lysine isophthalamide backbone grafted with the hydrophobic amino acid L- phenylalanine at a stoichiometric percentage of 50 mol%, was capable of delivering FITC- dextran, peptides and antibodies into the cell interior when the pH environment was lowered to pH 6.5. Additionally, the ability of PP50 to facilitate the uptake of the ribosome-inactivating protein saporin to induce apoptosis and cell death in 2D monolayer and multicellular spheroids highlights the potential use of PP50 for a wide range of therapeutic applications. PLP-NDA18, another anionic polymer comprised of a poly-L-lysine isophthalamide backbone grafted with decylamine at 18 mol%, also showed enhanced uptake and cytotoxicity of saporin when co-incubated with cells. For cell engineering applications, two cationic polymers, CP1 and CP2, were investigated for their ability to improve the direct cytoplasmic delivery of the target antigen ovalbumin peptide and induce antigen presentation by DCs. Whilst CP1 displayed superior membrane lytic capabilities at pH 6.5, CP2 showed to be the most promising polymer for the upregulation of MHC I and cytoplasmic delivery of the antigen peptide. In the future, a more comprehensive investigation into the underlying mechanisms of cationic mediated antigen delivery will help to understand the full capabilities of these cationic polymers as intracellular delivery platforms. Altogether, this work demonstrates that pH-responsive polymers are promising candidates as intracellular delivery platforms for a wide range of applications. Further studies and research are required for each polymer to reach its full potential as a widely adopted intracellular delivery agent.
Kρανιοεγκεφαλικές Κακώσεις Κατά την Διάρκεια Ερασιτεχνικής Άθλησης-Φυσικής Δραστηριότητας
Creators: name: Σύρμος, Νικόλαος Χ.
Publisher: name: Aristotle University of Thessaloniki
Year: 2025
Subjects: Νευροχειρουργική
Τραυματολογία
Κρανιοεγκεφαλικές Κακώσεις
Neurosurgery
Traumatiology
Traumatic Brain and Skull Injuries

description: Eισαγωγή: Οι κρανιοεγκεφαλικές κακώσεις αποτελούν σοβαρές τραυματικές καταστάσεις που επηρεάζουν σημαντικά την ποιότητα της ζωής και την σχετιζόμενη με την υγεία, ποιότητα ζωής των ερασιτεχνών αθλούμενων. Eπιδρούν επίσης αρνητικά, στην αθλητική επίδοση και απόδοση. Σκοπός: Σκοπός της παρούσας μελέτης είναι η καταγραφή και η ανάλυση περιπτώσεων τραυματισμών κατά την διάρκεια ερασιτεχνικής άθλησης-φυσικής δραστηριότητας, που προσέρχονται σε δευτεροβάθμιο Νοσοκομείο του Ελληνικού Εθνικού Συστήματος Υγείας. Υλικό-Μέθοδος: Αναλύονται 282 περιπτώσεις τραυματισμών σε βάθος καταγραφής πέντε ετών (2015-2016-2017-2018-2019). Aφορούν 202 άτομα ανδρικού φύλου (ποσοστό 71,6%) και 80 άτομα γυναικείου φύλου (ποσοστό 28,4%), με εύρος ηλικίας 9-80 έτη και μέσο όρο ηλικίας τα 41,5 έτη. Αποτελέσματα - Συμπεράσματα: Διαχρονικά, καταγράφεται αύξηση του αριθμού των τραυματισμών. Οι περισσότερες περιπτώσεις αφορούν άρρενες αθλούμενους, 20-40 ετών, κατά την διάρκεια του Θέρους και σε ημέρες αργίας. Οι κακώσεις εμφανίζονται κυρίως στις ενάλιες αθλητικές δραστηριότητες, στην χειμερινή άθληση, στα αθλήματα που εμπλέκουν δίκυκλα οχήματα, στα ομαδικά αθλήματα επαφής και στα αθλήματα μάχης. Η πρόληψη συντελεί στην αποφυγή σοβαρών τραυματισμών. Η συμβολή της Νευροχειρουργικής Ειδικότητας κρίνεται καίρια στην αντιμετώπιση των τραυματικών συμβάντων.
description: Ιntroduction: Traumatic skull and brain injuries are serious traumatic conditions that significantly affect the quality of life and the health-related quality of life of the affected amateur athletes. They also have a negative effect on athletic performancee and output. Aim: The purpose of this study is to record and analyze traumatic cases during amateur sports activity-physical exercise that they come to a secondary Hospital of the Hellenic National Health System. Material - Methods: 282 cases of injuries are analyzed over five years of recording (2015-2016-2017-2018-2019). They concern 202 men (71.6%) and 80 women (28.4%), with an age range of 9-80 years and an average age of 41.5 years. Results - Conclusions: Over time, an increase in the number of injuries is recorded. Most cases involve men, between 20-40 years, in the summer months and on non-working days. Ιnjuries occur mainly during Sports Sea Activities, Winter Sports, Cycling Activities, Team Contact Sports and Combat Sports. Prevention helps to avoid serious injuries. The contribution of the Neurosurgery Specialty is crucial in dealing with traumatic events.
CALCULATION OF THE PARAMETERS OF THE SEED-SEEDING MACHINE
Creators: name: A.V. Nikulin
name: E.A. Lukashin
name: A.V. Kuznetsov
name: A.A. Anikin
name: N.A. Makov
name: M.D. Anokhin
Publisher: name: Zenodo
Year: 2025

description: The article discusses a method for modernizing the seed-seeding apparatus of a grain planter of the SZ family. The article highlights the theoretical studies of the operation of a seed-seeding apparatus equipped with a designed coil. The designed coil is characterized by an enlarged groove shape, which allows it to operate at lower shaft speeds of the seeders. Also, to intensify the movement of seeds in the active layer, the shape of the ribs of the grooves has been changed. The results of calculations of the operating and technological parameters of a seed-seeding apparatus with a designed coil are presented.
Evaluation of glial activation in alzheimer’s trajectory using novel tspo pet marker, [18f]-ge180, and novel astrocyte marker [11c]-bu99008; evaluating its influence on microstructural alterations
Creators: name: Calsolaro, Valeria
Publisher: name: Imperial College London
Year: 2021

description: Background. The neuropathology of Alzheimer’s disease (AD) is still not fully understood. The activation of microglia and astrocytes plays a significant role in neurodegeneration, with debated relationship with amyloid, tau deposition, atrophy and glucose metabolism. This project evaluated two different PET tracers targeting microglia and astrocytes in volunteers with AD or MCI, and the relationship between microstructural alteration (fractional anisotropy and mean diffusivity) and microglial activation. Methods. The TSPO [18F]-GE180 and the Imidazolin-2 binding-site [11C]-BU99008 tracers were examined in volunteers with cognitive impairment (AD/MCI) and healthy controls (HC). The subjects underwent neuropsychological testing, MRI, amyloid-β PET scans, [18F]-GE180 or [11C]-BU99008 PET scan, [18F]-FDG PET scan (for the astrocyte study). For [18F]-GE180 and [11C]-BU99008, spectral analysis was used; SUVR analysis was conducted for [18F]- flutemetamol, [18F]-florbetaben and [18F]-FDG. DTI analysis (fractional anisotropy, FA, and mean diffusivity, MD) was performed for the GE180 study. Group comparisons and correlations analysis were done at regional and voxel-level. Results: [18F]-GE180 and [11C]-BU99008 uptake were higher in AD and MCI compared to HC, particularly in the amyloid-positive patients. Positive correlation was found between hippocampal volumes and [18F]-GE180 in AD and MCI. Reduced FA and increased MD were found in AD and MCI subjects compared to HC. No correlation was found between DTI measures and [18F]-GE180 uptake. Positive correlation was found between [11C]-BU99008 IRF120 and [18F]-FDG in the disease group; negative correlation was found between [11C]-BU99008 IRF120 and [18F]- florbetaben in occipital lobe and amygdala and between [11C]-BU99008 IRF120 in medial temporal lobes and [18F]-florbetaben in amygdala. 13 Conclusions. Microglial and astrocytes activation represent a common feature in the neurodegenerative trajectory of AD, with complex interplay between the hallmark of AD. The low penetration of the [18F]-GE180 tracer in the brain represents a limitation for its use. In vivo quantification of neuroinflammation remains crucial, considering its important role in neurodegeneration.
Static and cyclic characterisation of sandy and silty soils deposited underwater
Creators: name: Dominguez Quintans, Camelia
Publisher: name: Imperial College London
Year: 2022

description: Soils deposited underwater are typical of natural environments such as alluvial, fluvial, and offshore soils, or man-made infrastructure, like tailings dams. The preponderance of this type of deposition have made them a key target of soil mechanics research for many years. This study investigated the behaviour of sandy and silty materials deposited underwater using a comprehensive approach which aims to coordinate and critically analyse experimental and numerical practices from a macro and micromechanics perspective, by trying to evaluate the role of fabric in the soil´s behaviour. A novel setup was presented for the slurry deposition method that simulates underwater deposition, and can obtain high-quality, uniform specimens of sands or silts, the latter of which had never been done before. Mechanical behaviour from specimens reconstituted with this method were compared with the widely used moist tamping method, showing a strong effect on strength and stiffness owing to the initial fabric that results from the reconstitution method. An innovative analysis of the procedures required to establish the critical state line of sands based on small-strain data was also introduced. A new proposal for the assessment of critical states using phase transformation and peak states data was assessed through single element simulations. The use of the optimised calibration approach for the critical state line within a bounding surface plasticity model showed a better than usual performance to model key states of undrained triaxial shearing data over a wide range of densities and effective stress levels. This research also investigated the behaviour of slurry-deposited specimens of sands subjected to cyclic loading. The anisotropic nature of the specimens tested was revealed in monotonic loading, therefore in the boundary that triggered deformations in cyclic loading. The challenges of high-frequency triaxial testing were analysed, and the effects on the test results of inappropriate system performance were presented. The effect of frequency on the results of isotropically consolidated tests carried out with frequencies ranging from 0.1 Hz up to 5 Hz suggested a moderate increase in cyclic resistance as frequency increases for this material and the testing conditions used. Additionally, the study explored the applicability of multidirectional bender element testing in the assessment of the initial fabric and its evolution of granular materials in relation to all previous macroscale investigation factors and features, including the reconstitution method, monotonic shearing, and cyclic loading behaviour. Shear wave velocities in different axes were able to detect consistent differences between methods and distinct variations throughout loading.
RASSOM GIMNASTIKACHILARNING IJRO MAHORATI VA ULARNI BAHOLASH USULLARI
Creators: name: Baxauatdinova Nargiza
Publisher: name: Zenodo
Year: 2024

description: Rassom gimnastikachilarning ijro mahorati va ularni baholash usullari. Sportchilarning zamonaviy raqobatbardosh kompozitsiyalarida namoyon boʻladigan badiiylik va ekspressivlik tarkibiy qismlarining ahamiyatini oshirish.
Towards mass customising respiratory protective equipment via additive manufacture: a scalable and automated design process
Creators: name: Li, Shiya
Publisher: name: Imperial College London
Year: 2021

description: Respiratory Protective Equipment (RPE) such as disposable N95/FFP3 masks used in the healthcare industry, or re-usable elastomeric respirator masks used in construction, oil & gas, firefighting industries require an effective seal to ensure best performance. However, problems such as poor compliance and skin injuries have been commonly observed among users as existing commercial RPE fit poorly on individuals’ faces. Additive Manufacture (AM) has been proposed as a promising alternative to produce custom-fitted RPE for individuals. However, the cost of AM remains prohibitive for adoption at scale. While research studies have been mainly focused on the development of better and more efficient AM technologies, little has been done to make the AM design process more cost-efficient and scalable. Of the existing design methods, most have been semi-automated processes which still involve considerable design time and manual efforts. This thesis aims to develop a scalable and automated AM design process, and therefore making it more feasible to adopt AM for the mass customisation of RPE. Specifically, four data acquisition methods were evaluated to identify an appropriate one for obtaining geometrically accurate 3-Dimensoinal facial data with minimal resources needed. More importantly, an automated design pipeline based on the use of a template facial mesh was developed to bring facial meshes into dense correspondence and create customised RPE Computer-Aided Design models in large quantities. The template facial mesh was subsequently optimised considering geometric accuracy, surface quality of the fitted mesh and computational time. Next, the universality of the pipeline has been validated against a database of 205 facial scans obtained from people of different demographic backgrounds. Finally, the pipeline was further validated through conducting physical experiments to compare the performance of the 3D printed custom-fitted RPE against commercial RPE.
Mass Spectrometry Analysis of Isotopic Abundance of 13 C, 2 H, or 15 N in Biofield Energy Treated Aminopyridine Derivatives
Creators: name: Trivedi, Mahendra
Publisher: name: SMN - The ScienceMedia Network
Year: 2016
Subjects: Natural Sciences
Chemistry

description: 2-Aminopyridine (2-AP) and 2,6-diaminopyridine (2,6-DAP) are two derivatives of aminopyridines that act as an important organic intermediates, mostly used in medicines, dyes and organic sensors. The aim of the study was to evaluate the impact of biofield energy treatment on isotopic abundance ratios of 2H/1H, 13C/12C, or 15N/14N, in aminopyridine derivatives using gas chromatography-mass spectrometry (GC-MS). The 2-AP and 2,6-DAP samples were divided into two parts: control and treated. The control sample remained as untreated, while the treated sample was further divided into four groups as T1, T2, T3, and T4. The treated group was subjected to Mr. Trivedi’s biofield energy treatment. The GC-MS spectra of 2-AP and 2,6- DAP showed five and six m/z peaks respectively due to the molecular ion peak and fragmented peaks of aminopyridine derivatives. The isotopic abundance ratio of 2H/1H, 13C/12C, or 15N/14N were calculated for both the derivatives and significant alteration was found in the treated samples as compared to the respective control. The isotopic abundance ratio of 2H/1H, 13C/12C, or 15N/14N in treated samples of 2-AP was decreased by 55.83% in T1 and significantly increased by 202.26% in T4. However, in case of 2,6-DAP, the isotopic abundance ratio of 2H/1H, 13C/12C, and 15N/14N, in the treated sample showed a significant increase (up to 370.54% in T3) with respect to the control. GC-MS data suggested that the biofield energy treatment on aminopyridine derivatives had significantly altered the isotopic abundance of 2H, 13C, or 15N in the treated 2-AP and 2,6- DAP as compared to the control.
Supplementary document for Real-time phase imaging with self-supervised learning and equivariance - 6361517.pdf
Creators: name: Wang, Yuheng
name: Wang, Huiyang
name: Zhou, Chengxin
name: xianxin, han
name: Liu, Shengde
name: Lu, Xiaoxu
name: Di, Jianglei
name: zhong, Liyun
Publisher: name: Optica Publishing Group
Year: 2025
Subjects: Uncategorized

description: Comparative results of ablation studies.
title: Press release: Charles R. Fenwick; December 26, 1958
Creators: name: Fenwick, Charles R.
Publisher: name: George Mason University
Year: 2006
Subjects: subject: Welfare

description: Press release: 2 pgs; typescript unsigned; 8.5" x 11" (21.6cm x 27.9cm)
description: Press release by Charles R. Fenwick, senator from Virginia, regarding a federal mandate to apply funds uniformly for aid to dependent children. C. Harrison Mann, Jr. Collection 15.23
Compact modular open platform for low-cost ultrasound imaging
Creators: name: Kamal Tageldeen Mohammedosman, Momen
Publisher: name: Imperial College London
Year: 2022

description: Ultrasound open platforms (OPs) offer the flexibility and reconfigurability required for developing novel ultrasound techniques. However, they are generally large and expensive. This limits their use in primary care and low-resource settings. In this thesis, we propose a modular OP to address these challenges. The modular architecture of the proposed OP enables trading off size and cost to meet performance targets. The OP is composed of five core modules and is suitable for a wide range of ultrasound applications. The intrinsic modularity of the OP enables the extension of its performance by adding extension modules. Furthermore, the OP features a digital back-end controller that is designed specifically for ultrasound imaging and implemented on an Artix-7 FPGA. In particular, the OP is capable of generating arbitrary waveforms with a maximum frequency of 20 MHz and a maximum amplitude of $\pm100$ V. It can also generate precise delays with a maximum error of 200 ps. Additionally, the OP enables varying the gain from 12 dB to 51 dB. It also has a maximum ADC resolution of 12 bits, and a maximum conversion rate of 100 MSPS. Results from various phantoms and in vivo experiments demonstrate that the OP yields high-quality images and enables the detection of anatomical structures in small animals. Additionally, the OP has been deployed in two research projects, where it was shown to fulfil the requirements of both projects at a fraction of the cost and size of commercial OPs.  Finally, a novel analogue accelerator for the training of AI models using continuous-time stochastic gradient descent is proposed. The accelerator is intended for applications that demand privacy and on-device training, such as ultrasound imaging. The accelerator was simulated in AMS 0.35 um technology. Results show that the accelerator offers excellent accuracy, with at least 8 bits of resolution.
Some adamantyl-stabilised reactive intermediates
Creators: name: O'Reilly, Daniel
Publisher: name: Imperial College London
Year: 2022

description: The first preparative example and crystallographic characterisation of a 2-tert- alkyldioxolan-2-ylium ion salt (111) is reported herein. Oxonium ion 111 was found to be the end-point of a novel diastereoconvergent system – the first example of this behaviour in oxonium ions of any type – and was formed as the final product in hydride abstraction of either of diasteromeric dioxolanes 100 and 101 and in the halide abstraction of either of diastereomeric bromohydrin ester 123 and 124 (Ch 1). Evidence indirectly supporting an intermediate bromocyclopropanone in the thermal rearrangements of bromoallene oxides is presented (Ch 2).
title: Digital Campus Podcast - Episode 60 – Stimulus Plan
Creators: name: Center For History And New Media
Publisher: name: George Mason University
Year: 2010

description: Originally published by the Center for History and New Media through the Digital Campus podcast (http://digitalcampus.tv). This work is licensed under a Creative Commons Attribution 3.0 United States License (http://creativecommons.org/licenses/by/3.0/us/).
description: Dan, Tom, Mills, and Amanda return to discuss what's new for faculty this semester, including some welcome hiring in digital humanities. We discuss the trend of "cluster hiring" at big universities such as the one being advertised at Iowa and parallel developments at smaller colleges like Hamilton and Amherst [.doc]. Other topics include Google Instant [...]
Biophysical investigation of temporal interference neuromodulation
Creators: name: Luff, Charlotte Emily
Publisher: name: Imperial College London
Year: 2022

description: Temporal Interference (TI) stimulation is a non-invasive deep brain stimulation technique that has promise for treating brain diseases caused by aberrant neural activity. During TI, two high frequency electric fields are applied to the brain. These fields differ in frequency by a small amount termed the difference frequency. Neurons are stimulated at the difference frequency, however, it has not been determined how this occurs. The mechanism of action of TI is currently unknown, and elucidation of this is pivotal for development of the technique. In this thesis, I present work that probes the mechanisms of TI stimulation, and the nonlinear processes underlying it. Additionally, I investigate whether these processes are present in endogenous neural activity. I investigated the mechanism and parameter space of TI stimulation. I found that a neural frequency conversion process that originates at the single neuron membrane, is occurring during stimulation, resulting in subthreshold polarisation at the difference frequency. This nonlinear frequency conversion occurs at a wide range of applied frequencies, suggesting that it is being mediated by Frequency Mixing (FM). The strength of this nonlinear process and thus the strength of TI stimulation, is dependent on the applied frequency, but not on the difference frequency. Additionally, I found that the strength of TI stimulation is dependent on the summed amplitude of the applied currents. These findings aid in the elucidation of the mechanisms by which TI exerts its effects, which may help development of the technique, and have implications for the focality of the stimulation. Additionally, I investigated the presence of FM in endogenous neural activity by testing a new methodology for detection of FM via a three-way phase relationship between the signals involved. I found evidence for this nonlinear process in human cortical activity. I suggest that it may be disturbed in Traumatic Brain Injury (TBI), where large-scale functional networks are disrupted. Lastly, I validated a novel technique for non invasive deep brain stimulation called Pulse-Width Modulated-Temporal Interference (PWM-TI). I found that PWM-TI can effectively stimulate neurons at the difference frequency, with comparable efficiency to traditional TI.
Implementing An Exact Algorithm For Helical Ct:Removing The Cone-Beam Artefacts
Creators: name: Moe, Yngve M.
name: Lloyd, Ryan
name: Tregidgo, Henry
name: Coban, Sophia B.
name: Gajjar, Parmesh
name: Behnsen, Julia
name: Turner, Martin
name: Lionheart, William R.B.
Publisher: name: Zenodo
Year: 2016
Subjects: helical scan
cone beam CT
Katsevick
Nikon Xtek
artefacts

description: 3-dimensional computerised tomography is usually reconstructed using the naïve Feldkamp-Davis-Kress algorithm or FDK, which is not an exact reconstruction algorithm. This caus-

es image errors, or artefacts that appear out of the central slice, and are especially visible near edges between horizontal layers - this is demonstrated in Figure 1.

To overcome these artefacts; one can perform multiple scans at different heights and combine the reconstructed volumes. However, exact reconstruction methods are necessary to

remove them altogether. These algorithms will also reduce the scanning time which makes them very attractive. Such methods exist, and Alexander Katsevich provided an algo-

rithm already in 2002[1]. His algorithm uses a helical scan-geometry, rotating the X-Ray source while moving it in parallel with the rotation axis - or, equivalently, scanning the ob-

ject while rotating it and moving it parallel to its rotation axis. In this poster, we demonstrate that it is possible to perform and reconstruct such scans, with a Nikon XTEK scanner

designed for circular scans.
description: {"references": ["Alexander Katsevich. Theoretically exact filtered backprojection-type inversion algorithm for spiral CT. SIAM Journal on Applied Mathematics, 62(6):2012\u20132026, 2002.", "Henry F.J. Tregidgo. The Implementation and analysis of Katsevich Reconstruction for helical CT. MSc. thesis, The University of Manchester, 2013.", "Adam. J. Wunderlich. The Katsevich Inversion Formula for Cone-Beam Computed Tomography. MSc. dissertation, Oregon State University, 2006.21"]}
Baccalaureate Service, 1947
Creators: name: State Teachers College
Publisher: name: Maryland Shared Open Access Repository
Year: 1947
Subjects: subject: State Teachers College
subject: Commencement ceremonies

description: Baccalaureate service program for June 5, 1947, which may include written corrections and annotations. While every effort is made to ensure accuracy in baccalaureate service programs, the service may have changed after the program was printed.
Development and validation of a high-throughput mass spectrometric platform for the molecular characterisation of cells
Creators: name: Maneta Stavrakaki, Stefania
Publisher: name: Imperial College London
Year: 2022

description: Understanding the dynamic cellular metabolism is crucial for gaining deeper knowledge of the intracellular and intercellular functions, as well as the influence of microenvironment on cells. Mass Spectrometry has been used extensively for metabolomic and lipidomic analysis of cells, especially when hyphenated with separation techniques such as liquid chromatography (LC-MS). These methods are very sensitive and can give vital information regarding the lipidome and metabolome of cells. However, the sample preparation needed for these techniques is laborious and time-consuming and does not allow for real-time analysis of cells. Laser-Desorption Rapid Evaporative Ionisation Mass Spectrometry (LD-REIMS) is an ambient ionisation technique utilising infrared (IR) lasers for the generation of molecule-rich aerosol, which can be analysed with mass spectrometry methods. This PhD thesis aims to expand the application of the automated LD-REIMS platform for the molecular characterisation of cells with minimal or no sample preparation. A systematic comparison was performed between REIMS modalities that have been used previously for cell pellet analysis – bipolar forceps and cell probe REIMS –, and the LD-REIMS autosampler, – while a protocol for the preparation-free analysis of living or frozen cells was also developed and validated. LD-REIMS outperformed other REIMS modalities in terms of classification, molecular coverage, and repeatability when used for the analysis of 5 breast and 5 colorectal cancer cell lines. The applicability of LD-REIMS was demonstrated for the analysis of cells producing biopharmaceutical compounds, where metabolic differences were demonstrated between high and low producing cell lines. LD-REIMS was also used for the detection of significant differences in the lipid metabolism of phospholipidosis-induced cells, revealing upregulation of polyunsaturated fatty acids (PUFA) and the presence of Bis(Monoacylglycero)Phosphate (BMP) lipids. In conclusion, this PhD thesis demonstrates the potential of LD-REIMS for the high-throughput analysis of cells with minimal sample preparation, and the preparation-free analysis of living cells.
Designing efficient and localisable gene drives for population suppression
Creators: name: Willis, Katie
Publisher: name: Imperial College London
Year: 2022

description: Some species cause significant harm owing to their ability to transmit disease, inflict damage and cause unwanted changes to the environment. Although a range of population control strategies exist, significant burden remains, and novel approaches are sorely needed. Genetic population control is a fast-developing field which involves releasing genetically modified organisms to reduce the reproductive capacity of wild populations. Of particular interest are synthetic gene drive constructs which offer efficient control owing to their ability to increase in frequency from rare and spread to neighbouring populations via migration. However, sometimes only local populations need control, and impact outside of the release area would be undesirable. In this study we aimed to identify and evaluate novel efficient and localisable strategies for population suppression and develop a systematic approach to strategy discovery. Using mathematical models, we explored a range of two-construct strategies which exploit differentiated sequences in the genome to prevent spread, finding that they offer highly efficient localisable suppression. We also developed a flexible simulator capable of modelling strategies built from combinations of different molecular components and genes. With this, we screened hundreds of thousands of possible strategies and identified a range of self-limiting designs whilst also revealing general principles which govern their persistence and suppression potential. We also identified a novel category of threshold- dependent self-sustaining drives, which offer high levels of localised suppression with achievable release thresholds. Finally, we performed an in-depth analysis of a category of self- limiting strategies found to offer efficient suppression when released under a repeat-release regime and explored various augmentations which improve efficiency. Overall, this work revealed a range of novel strategies for achieving efficient, yet localisable population control; produced a highly flexible simulator; and validated a new approach for strategy discovery, which combines theoretical understanding and computational power to uncover previously unknown control strategies.
title: Cultural Influences on the Use of Biomedical Reproductive Healthcare in the Andean Highlands
Creators: name: Hrbacek, Tiffany
Publisher: name: George Mason University
Year: 2017
Subjects: subject: Women
subject: Reproductive health
subject: Body
subject: Andes
subject: Biomedicine
subject: Indigenous medicine

description: This thesis demonstrates some of the many ways that indigenous women in the Andean highlands are influenced to utilize indigenous medical and biomedical reproductive healthcare services and contraceptive methods. It focuses on some of the deeply rooted influences that affect choice of care, in particular, the cultural construction of the female body, gender roles and authority, the theories and practices of biomedicine in relation to those of indigenous Andean medicine, and the historical exploitation of indigenous Andean groups.
title: What Role Has Identity Played in the Fyrom-Greece Naming Dispute?
Creators: name: Miceli-Farrugia, Mark
Publisher: name: George Mason University
Year: 2017
Subjects: subject: Social identity
subject: Intergroup conflict
subject: Ethnicity
subject: Nationalism
subject: Populism
subject: Sovereignty

description: The role of identity in the naming dispute between FYROM and Greece is critical. The three main parties – the ethnic Macedonians and the ethnic Albanians in FYROM and the Greeks - each have their own identity needs: 1.1 the Christian Orthodox, ethnic (Slavophone) Macedonians wish to be finally recognized as a sovereign people after having been dominated by fellow-Slavs and Ottoman Turks for over 1,400 years; 1.2 the Muslim, ethnic Albanian Macedonians wish to be granted the political and economic rights of a minority group within Macedonia after 600 years of subjugation, first under the Muslim Turkish Ottoman Empire, later under the Orthodox Christian, Slavophone Macedonian majority; and 1.3 the Christian Orthodox Greeks wish to be recognized as the sole propagators of Hellenism, created and diffused worldwide by Kings Philip II and Alexander the Great of Macedon 2,400 years ago. The Muslim ethnic Albanian Macedonians are involved only indirectly in the naming dispute: firstly, because their support is essential to FYROM’s ruling, nationalist VRMO-DPMNE government; and secondly, because they are especially keen on securing protective rights as a minority community via FYROM’s accession to NATO and the EU. As we shall see, identity as “part of an individual’s self-concept,” generates emotional defensive or even offensive responses, when frustrated. The interplay of such emotions with the economic uncertainty prevailing in both countries can create incendiary circumstances. Under such circumstances, politicians might resort to scapegoating - unfairly blaming external third-parties or – often - discernible, internal minority groups.
[Kitamaat Mission broadsides]
Creators: name: Raley, G. H. (George Henry), 1864-1958
Publisher: name: Kitamaat, B.C. : Rev. G.H. Raley
Year: 2015

description: A collection of small broadsides, each printed with a passage from the Bible in both the Haisla and English languages (or sometimes the Haisla language only). These sheets were used for teaching purposes at the close of the Sunday morning service at the Mission. The collection also includes two programs of "Closing exercises" for June 1899 and June 1900.
title: Health Coaching and Motivation: Using the Health Belief Model to Explain Fitness Facility Members’ Barriers to Practicing Healthy Behaviors
Creators: name: Garrett, Amanda
Publisher: name: George Mason University
Year: 2018
Subjects: subject: Fitness
subject: Motivation
subject: Healthy behaviors
subject: Health coaching
subject: Health belief model
subject: Healthy behavior adherence

description: Practicing healthy behaviors may potentially lessen the occurrence of chronic diseases. However, individuals may report barriers that prevent them from practicing healthy behaviors. The Health Belief Model is utilized as the theoretical framework to guide the research by exploring in what manner fitness facility members’ perceived barriers will influence their willingness to practice healthy behaviors. Using an instrumental case study design, the researcher conducted in-person focus group interviews with 11 fitness professionals within the George Mason University Recreation Department. Respondents reported various barriers to individuals’ healthy behaviors, including time commitment, lack of motivation, financial constraints, intimidation and fear of inconsistent adherence. To assist members in overcoming reported barriers, respondents’ views on the importance of a health coaching program were addressed. Various aspects of health coaching’s importance are conferred, including health coaching’s influence on motivation, traits of quality health coaches, benefits of health coaching programs, structure of the health coaching programs and implementation challenges. Within the structure of the health coaching programs theme, sub-themes that are discussed include frequency of health coaching program meetings, components offered within health coaching programs, costs of the health coaching programs and campus collaborations. The results will be utilized for future development and implementation of a health coaching program for Mason Recreation.
Using landscape metrics to predict hydrologic connectivity patterns between forested wetlands and streams in a coastal plain watershed
Creators: name: Epting, Steven Michael
Publisher: name: Digital Repository at the University of Maryland
Year: 2016
Subjects: subject: Water resources management
subject: Environmental science
subject: Clean Water Act
subject: Delmarva bays
subject: Geographically isolated wetlands
subject: Geographic information systems
subject: Hydrologic connectivity
subject: Non-perennial streams

description: Geographically isolated wetlands, those entirely surrounded by uplands, provide numerous ecological functions, some of which are dependent on the degree to which they are hydrologically connected to nearby waters. There is a growing need for field-validated, landscape-scale approaches for classifying wetlands based on their expected degree of connectivity with stream networks. During the 2015 water year, flow duration was recorded in non-perennial streams (n = 23) connecting forested wetlands and nearby perennial streams on the Delmarva Peninsula (Maryland, USA). Field and GIS-derived landscape metrics (indicators of catchment, wetland, non-perennial stream, and soil characteristics) were assessed as predictors of wetland-stream connectivity (duration, seasonal onset and offset dates). Connection duration was most strongly correlated with non-perennial stream geomorphology and wetland characteristics. A final GIS-based stepwise regression model (adj-R2 = 0.74, p < 0.0001) described wetland-stream connection duration as a function of catchment area, wetland area and number, and soil available water storage.
Multimodal-Enhanced Objectness Learner for Corner Case Detection in Autonomous Driving
Creators: Lixing Xiao
Ruixiao Shi
Xiaoyang Tang
Yi Zhou
Publisher: name: IEEE Signal Processing Society
Year: 2024

description: Previous works on object detection have achieved high accuracy in closed-set scenarios, but their performance in open-world scenarios is not satisfactory. One of the challenging open-world problems is corner case detection in autonomous driving. Existing detectors struggle with these cases, relying heavily on visual appearance and exhibiting poor generalization ability. In this paper, we propose a solution by reducing the discrepancy between known and unknown classes and introduce a multimodal-enhanced objectness notion learner. Leveraging both vision-centric and image-text modalities, our semi-supervised learning framework imparts objectness knowledge to the student model, enabling class-aware detection. Our approach, Multimodal-Enhanced Objectness Learner (MENOL) for Corner Case Detection, significantly improves recall for novel classes with lower training costs. By achieving a 76.6% mAR-corner and 79.8% mAR-agnostic on the CODA-val dataset with just 5100 labeled training images, MENOL outperforms the baseline ORE by 71.3% and 60.6%, respectively.
title: Traditional Bullying and Cyberbullying among Adolescents: Patterns and Correlates of Victimization vs. Perpetration
Creators: name: Zhang, Lijing
Publisher: name: George Mason University
Year: 2021
Subjects: subject: Traditional and cyber bullying
subject: Victimization
subject: Risk and protective factors
subject: Adolescents
subject: Perpetration

description: Traditional bullying and cyberbullying are serious global public health concerns. Based on a national sample of 2,439 students from 30 public middle schools between the ages of 10 and 14 (M = 11.7 years) in the United States, we investigated patterns and predictors of four types of bullying (physical, verbal, social, and cyber) including both bullying victimization and perpetration. We also examined how demographic variables and several psychosocial risk and protective factors would be related to bullying victimization and perpetration. Overall, findings indicated that bullying victimization and perpetration were widespread, with verbal bullying the most frequent type of bullying both in terms of victimization and perpetration, and cyberbullying the least frequently reported. Boys were more likely to engage in physical bullying perpetration, while girls were more likely to report being victimized by relational (verbal and social) bullying. Findings indicated that knowing how to respond to bullying and acting in appropriate ways to bullying, or bullying prevention skills, were associated with less bullying perpetration. However, we found that conflict resolution skills were not associated with many forms of bullying. While the study included a large sample and measured multiple forms of bullying, limitations were the cross-sectional design which limited the ability to examine direction of causality. Future research should examine samples containing students from diverse communities and countries, assessing in greater detail the contexts in which bullying occur. This can help us understand how factors that place students at relative disadvantage interact with the environments in which students are bullied.
Impact of Biofield Energy Treatment on Soil Fertility
Creators: name: Trivedi, Mahendra
Publisher: name: SMN - The ScienceMedia Network
Year: 2016
Subjects: Natural Sciences
Earth sciences

description: Measurement of soil components such as microbial population, minerals and obviously the content of organic carbon play the important roles for the productivity of crops and plants. The present study was attempted to evaluate the impact of Mr. Trivedi’s biofield energy treatment on soil for its physical (electrical conductivity), chemical (minerals) and microbial flora (bacteria and fungi). A plot of lands was assigned for this study with some already grown plants. This plot was divided into two parts. One part was considered as control, while another part was subjected to Mr. Trivedi’s biofield energy treatment without physically touching and referred as treated. In the treated soil the total bacterial and fungal counts were increased by 546 and 617%, respectively as compared to the untreated soil. Additionally, the conductivity of soil of the treated plot was increased by 79% as compared to the soil of control plot. Apart from microbes, the content of various minerals were also changed in the biofield energy treated soil. The calcium carbonate content showed 2909 ppm in the control, while in the treated soil it was increased to 3943 ppm i.e. 36% increased. Various other minerals such as nitrogen and potassium were increased by 12% and 7%, respectively as compared to the control. Besides, the level of some minerals such as potassium, iron, and chloride were decreased by 9%, 23%, and 41%, respectively as compared to the control. Apart from chemical constituents of soil, the content of organic carbon was also reduced by 8% in the treated soil as compared to the control soil. The overall results envisaged that the biofield energy treatment on the soil showed a significant improvement in the physical, chemical, and microbial functions of soil component. Thus, improved the conductance, supportive microbes, minerals and overall productivity of crops. In conclusion, the biofield energy treatment could be used as an alternative way to increase the yield of quality crops by increasing soil fertility.
title: Information Sheet: House Beautiful Magazine, "House Beautiful's "Best Small House '84," 1984
Creators: name: Anonymous
Publisher: name: George Mason University
Year: 2006
Subjects: subject: Reston (Va.)

description: Information Sheet: Typescript original, 11" x 8.5" (29.7 cm x 21.6 cm)
description: Information Sheet from House Beautiful Magazine discussing their 1984 "Best Small House" competition. This item explains House Beautiful Magazine's reasoning for starting the small house competition in 1982 and the process through which a winning design was chosen. Also discussed was the 1984 winning design, submitted by Lester Walker of Woodstock, New York. This design, which was constructed in Reston, Virginia on a wooded half acre lot, called for a house with seven rooms that added up to 1,500 square feet. Planned Community Archives Collection, 457.10
Boundary between the Dominion of Canada and the territory of Alaska. Counter-case presented on the part of the government of His Britannic Majesty to the tribunal constituted under article I of the convention signed at Washington, January 24, 1903, between His Britannic Majesty and the United States of America
Creators: name: Great Britain
Publisher: name: London : Printed at the Foreign office, by Harrison and Sons
Year: 2016

Abstract
The Effect of Architecture and Shear Stress on Endothelialization of 3D Printed Vascular Networks
Creators: name: Talaie, Tara
Publisher: name: Digital Repository at the University of Maryland
Year: 2016
Subjects: subject: Biomedical engineering
subject: 3D Printing
subject: bioreactors
subject: endothelial cells
subject: HUVEC
subject: Tissue engineering
subject: tissue grafts

description: Despite significant progress in the field of tissue engineering within the last decade, a number of unsolved problems still remain. One of the most relevant issues is the lack of proper vascularization that limits the size of engineered tissues to smaller than clinically relevant dimensions. In particular, the growth of engineered tissue in vitro within bioreactors is plagued with this challenge. Specifically, the tubular perfusion system bioreactor has been used for large scale bone constructs; however these engineered constructs lack inherent vasculature and quickly develop a hypoxic core, where no nutrient exchange can occur, thus leading to cell death. Through the use of 3D printed vascular templates in conjunction with a tubular perfusion system bioreactor, we attempt to create an endothelial cell monolayer on 3D scaffolds that could potentially serve as the foundation of inherent vasculature within these engineered bone grafts.
Root patterns and exact surface energy of the spin-1 Heisenberg model with generic open boundaries
Creators: name: Dong, Jiasheng
name: Lu, Pengcheng
name: Cao, Junpeng
name: Yang, Wen-Li
name: Marquette, Ian
name: Zhang, Yao-Zhong
Publisher: name: La Trobe
Year: 2024
Subjects: classificationCode: 51
subject: Physical sciences
subject: FOS: Physical sciences
classificationCode: 49
subject: Mathematical sciences
classificationCode: 5106
subject: Nuclear and plasma physics
classificationCode: 4902
subject: Mathematical physics
classificationCode: 5103
subject: Classical physics

description: We investigate the thermodynamic limit and exact surface energy of the isotropic spin-1 Heisenberg chain with integrable generic open boundary conditions by a novel Bethe ansatz method. We obtain the homogeneous (or two-term) Bethe ansatz like equations for the zero roots of the transfer matrix. Based on the patterns of the zero roots, we analytical calculate the densities of zero roots and the surface energies of the model in all regimes of the boundary parameters.
title: Machine Learning Model to Detect Emergency in the Global Pandemic
Creators: name: Raju, Rini
Publisher: name: George Mason University
Year: 2021
Subjects: subject: COVID-19
subject: Machine learning

description: It is crucial to use advanced machine learning models to improve disaster and emergency response in critical events around the world. In this paper, we introduce a new model, which can highlight the essential help that people need in times of emergency. Based on the user comments, we choose the emergency response that can use the optimal resources to address the maximum needs. The new features in the model help to analyze each person's response from political, social, and health perspectives. This approach helps to recognize different types of users to improve emergency response in the time of the global pandemic. Also, collecting pandemic data from different online resources, makes this research more powerful in feature extraction to improve the model accuracy based on emergency data. This model can help health applications to improve disaster response time and services.
title: Fact Sheet: Shirley Highway, May 29, 1961
Creators: name: Fenwick, Charles R.
Publisher: name: George Mason University
Year: 2007
Subjects: subject: Traffic
subject: Transportation
subject: Broyhill, Joel T.

description: Fact Sheet: 6 pgs, typescript, 11" x 8.5" (27.9 cm x 21.6 cm)
description: Fact sheet on Shirley Highway by Sen. Charles R. Fenwick. The document gives an overview of the road's legislative history with regard to a lack of agreement between the Federal Government and the Commonwealth of Virginia on the maintenance and expansion of the road. C. Harrison Mann, Jr. Collection 34.14