Molecularly imprinted nanoparticles with recognition properties towards diphtheria toxin for ELISA applications
Creators: name: Alkanlı, Süleyman Serdar
name: Dal Yöntem, Fulya
name: Yaşar, Merve
name: Güven, Celal
name: Kahraman, M. Vezir
name: Apohan, Nilhan Kayaman
name: Aktaş, Zerrin
name: Öncül, Mustafa Oral
name: Ünlü, Ayhan
name: Akçakaya, Handan
Publisher: name: Taylor & Francis
Year: 2022
Subjects: subject: Biophysics
subject: Biochemistry
subject: Microbiology
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Pharmacology
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: Cancer
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Plastic antibodies can be used for <i>in vitro</i> neutralization of biomacromolecules with different fragments due to their potential in separation, purification, chemical sensor, catalysis and drug production studies. These polymer nanoparticles with binding affinity and selectivity comparable to natural antibodies were prepared using functional monomer synthesis and copolymerization of acrylic monomers <i>via</i> miniemulsion polymerization. As a result, the <i>in vitro</i> cytotoxic effect from diphtheria toxin was reduced by MIPs. <i>In vitro</i> imaging experiments of polymer nanoparticles (plastic antibodies) were performed to examine the interaction of diphtheria toxin with actin filaments, and MIPs inhibited diphtheria toxin damage on actin filaments. The enzyme-linked immunosorbent assay (ELISA) was performed with plastic antibodies labeled with biotin, and it was determined that plastic antibodies could also be used for diagnostic purposes. We report that molecularly imprinted polymers (MIPs), which are biocompatible polymer nanoparticles, can capture and reduce the effect of diphtheria toxic and its fragment A. Macromolecules can be imprinted by using their fragments as template molecules.MIPs gain an affinity for the template molecule by covalent binding, non-covalent interactions or ligand interactions, as well as the ability to bind, release and recognize the template molecule.The viability of cells treated with DT, NIPs and MIPs was determined by MTT assay.Immunofluorescence staining studies examined structural changes in actin filaments in HUVEC treated with DT, NIPs and MIPs.FA imprinted polymer has the ability to bind whole diphtheria toxin.FA-MIP gave significant results in terms of specificity in ELISA using diphtheria toxin. Macromolecules can be imprinted by using their fragments as template molecules. MIPs gain an affinity for the template molecule by covalent binding, non-covalent interactions or ligand interactions, as well as the ability to bind, release and recognize the template molecule. The viability of cells treated with DT, NIPs and MIPs was determined by MTT assay. Immunofluorescence staining studies examined structural changes in actin filaments in HUVEC treated with DT, NIPs and MIPs. FA imprinted polymer has the ability to bind whole diphtheria toxin. FA-MIP gave significant results in terms of specificity in ELISA using diphtheria toxin.