title: ÐÐ¾Ð¸ÑÐº Ð¼Ð¾Ð´ÑÐ»ÑÑÐ¾ÑÐ¾Ð² Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ STIM2-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼ÑÑ ÐºÐ°Ð»ÑÑÐ¸ÐµÐ²ÑÑ ÐºÐ°Ð½Ð°Ð»Ð¾Ð²
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Search of activity modulators of STIM2-dependent Calcium channels
Creators: name: ÐÑÐ¸Ð³Ð¾ÑÑÐµÐ², ÐÐ½Ð´ÑÐµÐ¹
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: ÐÐµÐ»ÐºÐ¸
ÐÐ¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð°Ñ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ñ
ÐÐ»ÐµÑÐºÐ° (Ð±Ð¸Ð¾Ð».)
ÐºÐ°Ð»ÑÑÐ¸Ð¹
Ð´ÐµÐ¿Ð¾-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼ÑÐ¹ Ð²Ñ Ð¾Ð´ ÐºÐ°Ð»ÑÑÐ¸Ñ
Ð±ÐµÐ»ÐºÐ¸ STIM Ð±ÐµÐ»ÐºÐ¸ Orai
Ð±Ð»Ð¾ÐºÐ°ÑÐ¾ÑÑ
Ð°Ð¼Ð¸Ð½Ð¾Ð¿Ð¾Ð»Ð¸ÐºÐ°ÑÐ±Ð¾Ð½Ð¾Ð²Ð°Ñ ÐºÐ¸ÑÐ»Ð¾ÑÐ°
Ð¶Ð¸Ð²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸
Ð¸Ð·Ð¼ÐµÑÐµÐ½Ð¸Ðµ ÐºÐ°Ð»ÑÑÐ¸Ñ

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð²ÐµÑÐµÑÑÐ² Ð¸Ð· ÑÐ¸Ð¼Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð±Ð¸Ð±Ð»Ð¸Ð¾ÑÐµÐºÐ¸ Ð°Ð½Ð°Ð»Ð¾Ð³Ð¾Ð² ÑÐ¾ÐµÐ´Ð¸Ð½ÐµÐ½Ð¸Ñ 4-ÐÐ Ð¢Ð¡ Ð½Ð° Ð´ÐµÐ¿Ð¾-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼ÑÐ¹ Ð²ÑÐ¾Ð´ ÐºÐ°Ð»ÑÑÐ¸Ñ. Ð¦ÐµÐ»ÑÑ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÐµÑÑÑ Ð½Ð°ÑÐ¾Ð¶Ð´ÐµÐ½Ð¸Ðµ Ð²ÐµÑÐµÑÑÐ²Ð°, Ð¾Ð±Ð»Ð°Ð´Ð°ÑÑÐµÐ³Ð¾ Ð»ÑÑÑÐ¸Ð¼Ð¸ Ð±Ð»Ð¾ÐºÐ°ÑÐ¾ÑÐ½ÑÐ¼Ð¸ Ð¸ ÑÐµÐ»ÐµÐºÑÐ¸Ð²Ð½ÑÐ¼Ð¸ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð°Ð¼Ð¸. ÐÐ»ÑÑÐµÐ²ÑÐ¼Ð¸ ÑÑÐ°ÑÑÐ½Ð¸ÐºÐ°Ð¼Ð¸ Ð´ÐµÐ¿Ð¾-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾Ð³Ð¾ Ð²ÑÐ¾Ð´Ð° ÐºÐ°Ð»ÑÑÐ¸Ñ ÑÐ²Ð»ÑÑÑÑÑ ÑÐµÐ½ÑÐ¾ÑÑ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ ÐºÐ°Ð»ÑÑÐ¸Ñ Ð² ÑÐ½Ð´Ð¾Ð¿Ð»Ð°Ð·Ð¼Ð°ÑÐ¸ÑÐµÑÐºÐ¾Ð¼ ÑÐµÑÐ¸ÐºÑÐ»ÑÐ¼Ðµ Ð±ÐµÐ»ÐºÐ¸ STIM (1/2) Ð¸ Ð±ÐµÐ»ÐºÐ¸ Orai (1/2/3), Ð½ÐµÐ¿Ð¾ÑÑÐµÐ´ÑÑÐ²ÐµÐ½Ð½Ð¾ ÑÐ¾ÑÐ¼Ð¸ÑÑÑÑÐ¸Ðµ ÐºÐ°Ð»ÑÑÐ¸ÐµÐ²ÑÐµ ÐºÐ°Ð½Ð°Ð»Ñ Ð½Ð° Ð¿Ð»Ð°Ð·Ð¼Ð°ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ðµ. ÐÐ°ÑÑÑÐµÐ½Ð¸Ñ Ð² ÐºÐ°Ð»ÑÑÐ¸ÐµÐ²Ð¾Ð¹ ÑÐ¸Ð³Ð½Ð°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð°ÑÑÐ¾ÑÐ¸Ð¸ÑÐ¾Ð²Ð°Ð½Ñ ÑÐ¾ Ð¼Ð½Ð¾Ð³Ð¸Ð¼Ð¸ Ð²Ð¸Ð´Ð°Ð¼Ð¸ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ð¹. Ð ÑÐ°Ð±Ð¾ÑÐµ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ Ð¼Ð¾Ð´ÐµÐ»ÑÐ½ÑÐµ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐµ Ð»Ð¸Ð½Ð¸Ð¸, ÑÐ¾Ð·Ð´Ð°Ð½Ð½ÑÐµ Ð½Ð° Ð±Ð°Ð·Ðµ ÐºÐ»ÐµÑÐ¾Ðº ÐÐÐ293. ÐÐ¸Ð½Ð°Ð¼Ð¸ÐºÐ° ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ ÐºÐ°Ð»ÑÑÐ¸Ñ Ð² ÐºÐ»ÐµÑÐºÐ°Ñ ÑÐµÐ³Ð¸ÑÑÑÐ¸ÑÐ¾Ð²Ð°Ð»Ð°ÑÑ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ½ÑÑ Ð·Ð¾Ð½Ð´Ð¾Ð² Fluo-4 AM Ð¸ Fura-2 AM. ÐÐ¾ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ°Ð¼ ÑÐºÑÐ¸Ð½Ð¸Ð½Ð³Ð° Ð±ÑÐ»Ð¸ Ð¾ÑÐ¾Ð±ÑÐ°Ð½Ñ Ð´Ð²Ð° ÑÐ¾ÐµÐ´Ð¸Ð½ÐµÐ½Ð¸Ñ, Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»ÑÐ½Ð¾ Ð¾Ð±Ð»Ð°Ð´Ð°ÑÑÐ¸Ðµ ÑÐµÐ»ÐµÐºÑÐ¸Ð²Ð½ÑÐ¼ Ð¿Ð¾Ð´Ð°Ð²Ð»ÑÑÑÐ¸Ð¼ ÑÑÑÐµÐºÑÐ¾Ð¼ Ð½Ð° Ð´ÐµÐ¿Ð¾-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼ÑÐ¹ Ð²ÑÐ¾Ð´ ÐºÐ°Ð»ÑÑÐ¸Ñ. ÐÐ´Ð½Ð¾ Ð¸Ð· ÑÐ¾ÐµÐ´Ð¸Ð½ÐµÐ½Ð¸Ð¹, Ð²ÐµÑÐ¾ÑÑÐ½Ð¾, Ð²Ð¾Ð·Ð´ÐµÐ¹ÑÑÐ²ÑÐµÑ Ð½Ð° Ð±ÐµÐ»ÐºÐ¸ Orai1 Ð¸Ð»Ð¸ Orai2, Ð½Ð¾ Ð½Ðµ Ð½Ð° Orai3. ÐÑÐ¾ÑÐ¾Ðµ Ð²ÑÐ±ÑÐ°Ð½Ð½Ð¾Ðµ ÑÐ¾ÐµÐ´Ð¸Ð½ÐµÐ½Ð¸Ðµ, Ð¿Ð¾Ð´Ð°Ð²Ð»ÑÐµÑ Ð´ÐµÐ¿Ð¾-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼ÑÐ¹ Ð²ÑÐ¾Ð´ ÐºÐ°Ð»ÑÑÐ¸Ñ, Ð°ÐºÑÐ¸Ð²Ð¸ÑÑÐµÐ¼ÑÐ¹ Ð±ÐµÐ»ÐºÐ¾Ð¼ STIM2, Ð¸ Ð¿ÑÐ°ÐºÑÐ¸ÑÐµÑÐºÐ¸ Ð½Ðµ Ð²Ð»Ð¸ÑÐµÑ Ð½Ð° Ð±ÐµÐ»Ð¾Ðº STIM1 Ð¸ Ð°ÐºÑÐ¸Ð²Ð¸ÑÑÐµÐ¼ÑÐµ Ð¸Ð¼ ÐºÐ°Ð½Ð°Ð»Ñ. ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾ÑÐ½ÑÑ Ð¸ ÑÐµÐ»ÐµÐºÑÐ¸Ð²Ð½ÑÑ ÑÐ²Ð¾Ð¹ÑÑÐ² Ð¾ÑÐ¾Ð±ÑÐ°Ð½Ð½ÑÑ ÑÐ¾ÐµÐ´Ð¸Ð½ÐµÐ½Ð¸Ð¹ Ð¿Ð¾Ð·Ð²Ð¾Ð»Ð¸Ñ ÑÐ°Ð·Ð´ÐµÐ»Ð¸ÑÑ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð±ÐµÐ»ÐºÐ¾Ð² STIM1 Ð¸ STIM2, ÑÑÐ¾ ÑÐ²Ð»ÑÐµÑÑÑ Ð°ÐºÑÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð·Ð°Ð´Ð°ÑÐµÐ¹ ÑÐ¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð¹ ÑÑÐ½Ð´Ð°Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð½Ð°ÑÐºÐ¸.
description: The given work is devoted to studying the impact of chemical compounds, similar to 4-MPTC, on store-operated Calcium entry. The goal of this work is finding a compound, that would have better selective and blocking properties than those of 4-MPTC. Key players in store-operated Calcium entry are STIM (1/2) proteins, which sense the calcium concentration in endoplasmic reticulum, and Orai (1/2/3) proteins, which forms the calcium channel on plasma membrane. Misregulations of calcium signaling are associated with number of different diseases. Model cell lines, based on HEK293 cells, were used in this work. Fluorescent indicators Fluo-4 AM and Fura-2 AM were used to detect changes in cytoplasmic Calcium concentration. Two compounds showed inhibitory effect on store-operated Calcium entry during screening. One of them potentially blocks Orai1 or Orai2 proteins and does affect Orai3 protein. Another selected compound suppresses the Calcium influx, caused by STIM2 protein, and has almost no affection on STIM1-dependent calcium entry. Further studying of selective and inhibitory properties of these compounds will allow dividing the activity of STIM1 and STIM2 proteins, which is the actual problem of contemporary fundamental science.