title: ÐÐ¿ÑÐ¸Ð¼Ð¸Ð·Ð°ÑÐ¸Ñ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ Ð¼ÑÐ»ÑÑÐ¸Ð¿Ð»ÐµÐºÑÐ½Ð¾Ð¹ ÐÐ¦Ð Ð² Ð¼Ð¸ÐºÑÐ¾ÑÐ»ÑÐ¸Ð´Ð½Ð¾Ð¼ ÑÐ¸Ð¿Ðµ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Optimization of multiplex PCR on a microfluidic chip
Creators: name: ÐÐ°ÑÑÑÐ°, ÐÐ»ÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: ÑÐµÑÐ¼Ð¾ÑÐ¸ÐºÐ»ÐµÑ
ÑÑ ÐµÐ¼Ð¾ÑÐµÑ Ð½Ð¸ÐºÐ°
Ð¿ÑÐ¸Ð±Ð¾ÑÐ¾ÑÑÑÐ¾ÐµÐ½Ð¸Ðµ
Ð¼ÑÐ»ÑÑÐ¸Ð¿Ð»ÐµÐºÑ
Ð¼Ð¸ÐºÑÐ¾ÑÐ»ÑÐ¸Ð´Ð¸ÐºÐ°
thermocycler
circuit engineering
instrumentation
multiplex
microfluidics

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description: This given work is devoted to optimizing multiplex PCR on a microfluidic chip. To achieve this goal was developed an independent control board of the thermocycler. Also, a series of experiments were conducted on the experimental stand to verify the correctness of PCR, the results were compared with the data of the ANK-32 nucleic acid analyzer based on PCR-RT, developed by IAP RAS. A series of experiments was carried out to select the optimal temperature regime for the reaction. During the optimization process, annealing temperatures of 96, 94, 92 and 90 Â°C were examined. During PCR on a thermocycler, data was obtained that confirmed the possibility of reducing the annealing temperature to 92 Â° C, a further decrease in the annealing temperature led to a decrease in the effectiveness of the method. At the moment, there are no completed solutions on the market for controlling the thermocycling process. In this study, the operability of experimental stand assembled on the basis of the designed board was confirmed. The problem of current stabilization and automation of temperature control were solved. This project can be successfully scaled and applied in other projects that require accurate and stable thermal control