Aplosporella abexaminans Sanjeet, Sanjay & Raghv. Singh 2021, sp. nov.
Creators: name: Verma, Sanjeet Kumar
name: Yadav, Sanjay
name: Singh, Raghvendra
Publisher: name: Zenodo
Year: 2021
Subjects: Biodiversity
Taxonomy
Fungi
Ascomycota
Dothideomycetes
Botryosphaeriales
Aplosporellaceae
Aplosporella
Aplosporella abexaminans

description: Aplosporella abexaminans Sanjeet, Sanjay & Raghv. Singh, sp. nov. (Fig 1–4)
MycoBank number: MB 837539
Diagnosis:— Aplosporella abexaminans is characterized by its very larger ascostromata, locules without ostioles, peridium of thinner locules, two to multi-layered, larger asci, surrounded by an additional hyaline sac like structure, 8- spored, larger ascospores, hyaline to light olivaceous, asexual state without conidiomata formation, conidiogenous cells brown, absence of paraphyses, conidia brown without granular content, smooth and presence of chlamydospores.
Etymology:— “ ab -” (Latin) means “away” or “separation” implied its similarity but difference to Bagnisiella examinans.
Type:— India, Madhya Pradesh, Panchmarhi, on the stem of Murraya koenigii L. (Rutaceae), 6 March 2018, coll. Sanjeet Kumar Verma, AMH 10212 (holotype), MH-BHU 09 (isotype), NFCCI 5010 (ex-type living culture).
Sexual morph: Ascostromata 0.5–2.5 mm wide, 0.25–0.45 mm height, stiff, black, leathery, gregarious, partially under the host surface and erumpent, circular to subcircular in outline, solitary to gregarious, coriaceous, flattened at the upper surface with many polygonal areas, multiloculate, with up to70 locules (each polygonal areas on the upper surface represents each locule), each locule contains single ascus, cells of ascostromata brown to dark brown-walled, textura angularis. Locules (70)80–100(115) × (16)20–25(30) μm, without ostioles. Peridium of locules 4–25 µm thick at the sides, two to multi-layered, dark brown, thick-walled cells, textura angularis, pseudoparenchymatous. Paraphysis absent. Asci (70)75–110(115) × (17)20–25(30) µm, cylindrical to cylindro-clavate, bitunicate, fissitunicate, surrounded by an additional hyaline sac like structure, 8-spored, apex rounded and thick-walled with a narrow inversely funnel-shaped ocular chamber, very short pedicellate (upto 30 µm). Ascospores (17)23–29(30) × 10–14 µm, over-lapping, 1 to 2-seriate, hyaline to light olivaceous, aseptate, ellipsoid to oval, smooth. Asexual morph: Conidiomata lack on PDA. Hyphae 2–6 µm wide, branched, septate, smooth to verruculose, subhyaline to light brown. Conidiophores develop as a lateral branch of creeping hyphae. Conidiogenous cells 15–19 × 3.5–6.5 μm, usually not distinct, sometimes cylindrical to phialidic, light brown to brown, smooth to slightly roughened. Conidia (10)16–18(20) × (6)9–10(12) μm, aseptate, mid brown to dark brown, smooth-walled, ellipsoid to oval, apex rounded, sometimes hilum with peg like projection. Chlamydospores 7–14 × 5–13 μm, mid brown to dark brown, spherical to oval.
Culture characteristics
Ascospores germinated on PDA within 24 h. Colonies on PDA attained a diameter of 9 cm after 21 days at 25˚ C, upper surface whitish to brownish with rounded brownish to blackish raised spots, reverse brown to black with round blackish spots, irregular, velvety and margin scattered.
Phylogeny
Based on a megablast search of NCBI GenBank nucleotide database, the closest hits using ITS and LSU sequences were Bagnisiella examinans strain CBS 551.66 (GenBank: EU 167562, Identities = 575/575 (100%), gap = 0/575 (0%)) and Bagnisiella examinans strain CBS 551.66 (GenBank: KF 766316, Identities = 788/789 (99%), gap = 1/789 (0%)) respectively.
Phylogenetic analysis based on ITS-LSU concatenated sequences (Fig. 6), Aplosporella abexaminans clustered closer (ML / MP /BPP: 98/98/0.99) to Bagnisiella examinans (Mont. & Berk.) Arx & E. Müll. (1975) among the members of Aplosporella in Aplosporellaceae. A best scoring RAxML tree is presented in Fig. 6, with the Likelihood value of −6121.803898. The most parsimonious tree showed length = 889 steps, consistency index = 0.414, retention index = 0.851 and the composite index is 0.413861 (0.353318) for all sites and parsimony-informative sites (in parentheses).
description: Published as part of Verma, Sanjeet Kumar, Yadav, Sanjay & Singh, Raghvendra, 2021, Molecular phylogeny of Aplosporella abexaminans: a novel species revealing the second report of sexual-asexual connection in Aplosporellaceae (Botryosphaeriales) from India, pp. 205-222 in Phytotaxa 525 (3) on pages 210-215, DOI: 10.11646/phytotaxa.525.3.3, http://zenodo.org/record/5712372