title: ÐÐ·ÑÑÐµÐ½Ð¸Ðµ ÑÐ²Ð¾Ð¹ÑÑÐ² Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð¹ ÑÐµÐ»Ð»ÑÐ»Ð¾Ð·Ñ Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð±Ð¸Ð¾ÐºÐ¾Ð½ÑÐµÐ¹Ð½ÐµÑÐ° Ð´Ð»Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÑÐ¿Ð¸ÑÐµÐ»Ð¸Ð°Ð»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÐºÐ¾Ð¶Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Studying the properties of bacterial cellulose as a biocontainer for cultivating skin epithelial cells
Creators: name: ÐÐ°Ð»Ð°ÑÐµÐ½ÐºÐ¾, ÐÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½Ð°Ñ ÑÐµÐ»Ð»ÑÐ»Ð¾Ð·Ð°
ÑÐ°Ð½ÐµÐ²ÑÐµ Ð¿Ð¾ÐºÑÑÑÐ¸Ñ
ÐºÐ»ÐµÑÐ¾ÑÐ½Ð°Ñ Ð»Ð¸Ð½Ð¸Ñ HaCaT
ÐºÐµÑÐ°ÑÐ¸Ð½Ð¾ÑÐ¸ÑÑ
ÑÐ¸Ð±ÑÐ¾Ð±Ð»Ð°ÑÑÑ
bacterial cellulose
wound coatings
HaCaT cell line
keratinocytes
fibroblasts

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð´Ð¸ÑÑÑÐ·Ð¸Ð¾Ð½Ð½ÑÑ Ð¸ Ð´ÑÑÐ³Ð¸Ñ ÑÐ²Ð¾Ð¹ÑÑÐ² Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð¹ ÑÐµÐ»Ð»ÑÐ»Ð¾Ð·Ñ Ð´Ð»Ñ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ¸ ÑÐ°Ð½ÐµÐ²ÑÑ Ð¿Ð¾ÐºÑÑÑÐ¸Ð¹. ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: 1. ÐÐ±Ð·Ð¾Ñ Ð½Ð°ÑÑÐ½Ð¾Ð¹ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÑ Ð¿Ð¾ ÑÐµÐ¼Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ; 2. ÐÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð´Ð¸ÑÑÑÐ·Ð¸Ð¾Ð½Ð½ÑÑ ÑÐ²Ð¾Ð¹ÑÑÐ² Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð¹ ÑÐµÐ»Ð»ÑÐ»Ð¾Ð·Ñ Ð¸ ÐµÐµ Ð²Ð»Ð°Ð³Ð¾ÐµÐ¼ÐºÐ¾ÑÑÐ¸; 3. ÐÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð»Ð¸Ð½Ð¸Ð¹ HaCaT, Ð¿ÐµÑÐ²Ð¸ÑÐ½ÑÑ Ð»Ð¸Ð½Ð¸Ð¹ ÐºÐµÑÐ°ÑÐ¸Ð½Ð¾ÑÐ¸ÑÐ¾Ð² Ð¸ ÑÐ¸Ð±ÑÐ¾Ð±Ð»Ð°ÑÑÐ¾Ð² Ð½Ð° Ð¿Ð¾Ð²ÐµÑÑÐ½Ð¾ÑÑÐ¸ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð¹ ÑÐµÐ»Ð»ÑÐ»Ð¾Ð·Ñ; 4. Ð¡ÑÐ°Ð²Ð½ÐµÐ½Ð¸Ðµ ÑÐ°Ð·Ð½ÑÑ ÑÐ¾ÑÐ¼ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð¹ ÑÐµÐ»Ð»ÑÐ»Ð¾Ð·Ñ Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð¼Ð°ÑÑÐ¸ÑÑ Ð´Ð»Ñ Ð²ÑÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ñ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ ÐºÑÐ»ÑÑÑÑ. ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ñ Ð½Ð° Ð±Ð°Ð·Ðµ Ð¾ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾Ð¹ Ð¸ ÑÐ°Ð´Ð¸Ð°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ Ð±Ð¸Ð¾ÑÐ¸Ð·Ð¸ÐºÐ¸ ÐÐÐ¦ Â«ÐÑÑÑÐ°ÑÐ¾Ð²ÑÐºÐ¸Ð¹ Ð¸Ð½ÑÑÐ¸ÑÑÑÂ» ÐÐÐ¯Ð¤ (Ð³. ÐÐ°ÑÑÐ¸Ð½Ð°, Ð Ð¾ÑÑÐ¸Ñ). Ð ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ Ð±ÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð´Ð¸ÑÑÑÐ·Ð¸Ñ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð¹ ÑÐµÐ»Ð»ÑÐ»Ð¾Ð·Ñ Ð¿ÑÐ¾ÑÐ¾Ð´Ð¸Ñ ÑÐ°Ð²Ð½Ð¾Ð¼ÐµÑÐ½Ð¾, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¸Ð¼ÐµÐµÑ Ð²ÑÑÐ¾ÐºÑÑ ÑÑÐµÐ¿ÐµÐ½Ñ Ð¸Ð´ÐµÐ½ÑÐ¸ÑÐ½Ð¾ÑÑÐ¸ Ð´Ð»Ñ Ð¿Ð»ÐµÐ½Ð¾Ðº Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð¹ ÑÐµÐ»Ð»ÑÐ»Ð¾Ð·Ñ Ð¾Ð´Ð½Ð¾Ð¹ ÑÐ¾Ð»ÑÐ¸Ð½Ñ. Ð¢Ð°ÐºÐ¶Ðµ Ð±ÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð²Ð»Ð°Ð³Ð¾ÐµÐ¼ÐºÐ¾ÑÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð¹ ÑÐµÐ»Ð»ÑÐ»Ð¾Ð·Ñ Ð·Ð°Ð²Ð¸ÑÐ¸Ñ Ð¾Ñ ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÑ Ð²ÑÑÑÑÐ¸Ð²Ð°Ð½Ð¸Ñ. ÐÑÐ¾Ð¼Ðµ ÑÐ¾Ð³Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ñ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐ¼Ð¸ Ð»Ð¸Ð½Ð¸ÑÐ¼Ð¸ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð»Ð¸ Ð¿ÑÐ¸Ð³Ð¾Ð´Ð½Ð¾ÑÑÑ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð¹ ÑÐµÐ»Ð»ÑÐ»Ð¾Ð·Ñ Ð´Ð»Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ñ Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð±Ð¸Ð¾ÐºÐ¾Ð½ÑÐµÐ¹Ð½ÐµÑÐ¾Ð², Ð² ÑÐ°ÑÑÐ½Ð¾ÑÑÐ¸ Ð¿Ð»ÐµÐ½Ð¾Ðº. ÐÑÐ¸ Ð½Ð°Ð¿Ð¸ÑÐ°Ð½Ð¸Ð¸ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±Ð·Ð¾ÑÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ: eLIBRARY.RU, PubMed, scholar.google. Ð¡ÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÑÑ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»Ð¸ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿Ð°ÐºÐµÑÐ° Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼ Microsoft Excel.
description: This work is devoted to the study of diffusion and other properties of bacterial cellulose for the development of wound dressings. Problems that were solved during the study: 1. Review of scientific literature on the research topic. 2. Determination of the diffusion properties of bacterial cellulose and its moisture capacity. 3. Cultivation of HaCaT cell lines, primary lines of keratinocytes and fibroblasts on the surface of bacterial cellulose. 4. Comparison of different forms of bacterial cellulose as a matrix for growing cell cultures. The studies were carried out on the basis of the Department of Molecular and Radiation Biophysics of the National Research Center "Kurchatov Institute" PNPI (Gatchina, Russia). During the studies, it was shown that bacterial cellulose diffusion occurs uniformly and also has a high degree of identity for bacterial cellulose films of the same thickness. It was also shown that the moisture capacity of bacterial cellulose depends on the drying temperature. In addition, studies with cell lines have shown the suitability of bacterial cellulose for use as cellular biocontainers, in particular films. When writing the literature review, the following databases were used: eLIBRARY.RU, PubMed, scholar.google Statistical processing of experimental data was carried out with using the Microsoft Excel software package.