Additional file 1 of Plasma neurofilament light and its association with all-cause mortality risk among urban middle-aged men and women
Creators: name: Beydoun, May A.
name: Noren Hooten, Nicole
name: Weiss, Jordan
name: Beydoun, Hind A.
name: Hossain, Sharmin
name: Evans, Michele K.
name: Zonderman, Alan B.
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Neuroscience
subject: Physiology
subject: FOS: Biological sciences
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics
subject: Cancer

description: Additional file 1: Fig. S1. Participant flowchart illustrating plasma NfL measurements and mortality for this study. Abbreviations: δ = Annualized change; HANDLS = Healthy Aging Neighborhoods of Diversity Across the Life Span; NfL = Neurofilament Light Chain. Method S1. NfL sample selection. Table S1. Allostatic load indicator criteria. Method S2. Mixed-effects regression models. Fig. S2. Observed vs. empirical bayes estimator for annualized rate of change in Loge transformed NfL, Pearson’s r = 0.83, p &lt; 0.001. Table S2. BMI, AL, AL continuous parameters and cardio-metabolic co-morbidity indices and their relation to all-cause mortality by sex: mediating and moderating effects of NfLv1 using 4-way decomposition a,b,c. Abbreviations: AL = Allostatic Load; ALB = Albumin; BMI = Body Mass Index; CDE = Controlled Direct Effect; CES-D = Center for Epidemiological Studies-Depression; CHOL = Total cholesterol; CRP = C-reactive protein (high sensitivity), Loge transformed; CVD = Cardiovascular Disease; DBP = Diastolic Blood Pressure; DIAB = Diabetes; HBA1C = Glycated Hemoglobin; HDL = High Density Lipoprotein-Cholesterol; HEI-2010 = Healthy Eating Index-2010 version; HYPERT = Hypertension; HYPERCHOL = Hypercholesterolemia; IM = Interaction, mediated; IR = Interaction, Reference; M = Mediators/Moderator; NfL = Plasma Neurofilament Light Chain, Loge transformed; PM-Pure Mediation; RHR = Resting Heart Rate; SBP = Systolic Blood Pressure; WHR = Waist-Hip Ratio; X = Exposure. a See Methods and Table 1 for definition of each NfL exposure (i.e., NfLv1 and δNfL). All exposures (X) and potential mediators/moderators (M) were z-scored for ease of interpretation, with the exception of binary M (coded as 0/1), namely DIAB, HYPERT, HYPERCHOL, CVD. Control variables were set at their means. b Cox models for which 4-way decomposition was conducted is equivalent to Model 2, Table 2, for continuous exposures, to which M was added and considered as a potential mediator/moderator. Control variables included age at v1, sex, race, poverty status, education, HEI-2010 total score, mean energy intake (kcal/d), current tobacco use, current illicit drug use, the CES-D total score and the inverse mills ratio. c Total effects are beta = Loge(HR)±SE with associated p-values from Cox PH hazards models associated with each exposure of interest. Hazard Ratios (HR) point estimates exponent of beta. 95% CI for HR can be calculated as follows: Lower confidence limit, LCL: exp[LogeHR-1.96SE(LogeHR)], upper confidence limit, UCL: exp[LogeHR+1.96SE(LogeHR)]. Table S3. BMI, AL, AL continuous parameters and cardio-metabolic co-morbidity indices and their relation to all-cause mortality by sex: mediating and moderating effects of δNfL using 4-way decomposition a,b,c. Abbreviations: AL = Allostatic Load; ALB = Albumin; BMI = Body Mass Index; CDE = Controlled Direct Effect; CES-D = Center for Epidemiological Studies-Depression; CHOL = Total cholesterol; CRP = C-reactive protein (high sensitivity), Loge transformed; CVD = Cardiovascular Disease; DBP = Diastolic Blood Pressure; DIAB = Diabetes; HBA1C = Glycated Hemoglobin; HDL = High Density Lipoprotein-Cholesterol; HEI-2010 = Healthy Eating Index-2010 version; HYPERT = Hypertension; HYPERCHOL = Hypercholesterolemia; IM = Interaction, mediated; IR = Interaction, Reference; M = Mediators/Moderator; NfL = Plasma Neurofilament Light Chain, Loge transformed; PM-Pure Mediation; RHR = Resting Heart Rate; SBP = Systolic Blood Pressure; WHR = Waist-Hip Ratio; X = Exposure. a See Methods and Table 1 for definition of each NfL exposure (i.e., NfLv1 and δNfL). All exposures (X) and potential mediators/moderators (M) were z-scored for ease of interpretation, with the exception of binary M (coded as 0/1), namely DIAB, HYPERT, HYPERCHOL, CVD. Control variables were set at their means. b Cox models for which 4-way decomposition was conducted is equivalent to Model 2, Table 2, for continuous exposures, to which M was added and considered as a potential mediator/moderator. Control variables included age at v1, sex, race, poverty status, education, HEI-2010 total score, mean energy intake (kcal/d), current tobacco use, current illicit drug use, the CES-D total score and the inverse mills ratio. c Total effects are beta = Loge(HR)±SE with associated p-values from Cox PH hazards models associated with each exposure of interest. Hazard Ratios (HR) point estimates exponent of beta. 95% CI for HR can be calculated as follows: Lower confidence limit, LCL: exp[LogeHR-1.96SE(LogeHR)], upper confidence limit, UCL: exp[LogeHR+1.96SE(LogeHR)].