cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Co-expressed Cyclin D variants cooperate to regulate proliferation of germline nuclei in a syncytium.
Creators: name: Gunasekaran Subramaniam
name: Campsteijn, Coen
name: Thompson, Eric M
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: Cancer
subject: Developmental Biology
subject: Earth and Environmental Sciences
subject: Evolutionary Biology
subject: FOS: Biological sciences
subject: Biotechnology
subject: Molecular Biology
subject: Cell Biology

description: ABSTRACTThe role of the G1-phase Cyclin D-CDK 4/6 regulatory module in linking germline stem cell (GSC) proliferation to nutrition is evolutionarily variable. In invertebrate Drosophila and C. elegans GSC models, G1 is nearly absent and Cyclin E is expressed throughout the cell cycle, whereas vertebrate spermatogonial stem cells have a distinct G1 and Cyclin D1 plays an important role in GSC renewal. In the invertebrate, chordate, Oikopleura, where germline nuclei proliferate asynchronously in a syncytium, we show a distinct G1-phase in which two Cyclin D variants are co-expressed. Cyclin Dd, present in both somatic endocycling cells and the germline, localized to germline nuclei during G1 before declining at G1/S. Cyclin Db, restricted to the germline, remained cytoplasmic, co-localizing in foci with the Cyclin-dependent Kinase Inhibitor, CKIa. These foci showed a preferential spatial distribution adjacent to syncytial germline nuclei at G1/S. During nutrient-restricted growth arrest, upregulated CKIa accumulated in arrested somatic endoreduplicative nuclei but did not do so in germline nuclei. In the latter context, Cyclin Dd levels gradually decreased. In contrast, the Cyclin Dbβ splice variant, lacking the Rb-interaction domain and phosphodegron, was specifically upregulated and the number of cytoplasmic foci containing this variant increased. This upregulation was dependent on stress response MAPK p38 signaling. We conclude that under favorable conditions, Cyclin Dbβ-CDK6 sequesters CKIa in the cytoplasm to cooperate with Cyclin Dd-CDK6 in promoting germline nuclear proliferation. Under nutrient-restriction, this sequestration function is enhanced to permit continued, though reduced, cycling of the germline during somatic growth arrest.
Evidence for an outer disk in the prototype "compact elliptical" galaxy M32
Creators: name: Graham, Alister
Publisher: name: Swinburne
Year: 2024

description: M32 is the prototype for the relatively rare class of galaxies referred to as compact ellipticals. It has been suggested that M32 may be a tidally disturbed r 1/4 elliptical galaxy or the remnant bulge of a disk-stripped early-type spiral galaxy. This Letter reveals that the surface brightness profile, the velocity dispersion measurements, and the estimated supermassive black hole mass in M32 are inconsistent with the galaxy having, and probably ever having had, an r 1/4 light profile. Instead, the radial surface brightness distribution of M32 resembles an almost perfect (bulge+exponential disk) profile; this is accompanied by a marked increase in the ellipticity profile and an associated change in the position angle profile where the 'disk' starts to dominate. Compelling evidence that this bulge/disk interpretation is accurate comes from the best-fitting r 1/n bulge model, which has a Sérsic index of n = 1.5, in agreement with the recently discovered relation between a bulge's Sérsic index and the mass of a bulge's supermassive black hole. An index of n > 4 would also be inconsistent with the stellar velocity dispersion of M32. The bulge-to-disk size ratio re/h equals 0.20, and the logarithm of the bulge-to-disk luminosity ratio log (B/D) equals 0.22, typical of SΦ galaxies. The effective radius of the bulge is 27 (100 pc), while the scale length of the disk is less well determined: owing to possible tidal stripping of the outer profile beyond 220' - 250', the scale length may be as large as 1.3 kpc. M32 is a relatively face-on, nucleated dwarf galaxy with a low surface brightness disk and a high surface brightness bulge. This finding brings into question the very existence of the compact elliptical class of galaxies.
Co-expressed Cyclin D variants cooperate to regulate proliferation of germline nuclei in a syncytium
Creators: name: Gunasekaran Subramaniam
name: Campsteijn, Coen
name: Thompson, Eric M
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: Cancer
subject: Developmental Biology
subject: Earth and Environmental Sciences
subject: Evolutionary Biology
subject: FOS: Biological sciences
subject: Biotechnology
subject: Molecular Biology
subject: Cell Biology

description: The role of the G1-phase Cyclin D-CDK 4/6 regulatory module in linking germline stem cell (GSC) proliferation to nutrition is evolutionarily variable. In invertebrate Drosophila and C. elegans GSC models, G1 is nearly absent and Cyclin E is expressed throughout the cell cycle, whereas vertebrate spermatogonial stem cells have a distinct G1 and Cyclin D1 plays an important role in GSC renewal. In the invertebrate, chordate, Oikopleura, where germline nuclei proliferate asynchronously in a syncytium, we show a distinct G1-phase in which 2 Cyclin D variants are co-expressed. Cyclin Dd, present in both somatic endocycling cells and the germline, localized to germline nuclei during G1 before declining at G1/S. Cyclin Db, restricted to the germline, remained cytoplasmic, co-localizing in foci with the Cyclin-dependent Kinase Inhibitor, CKIa. These foci showed a preferential spatial distribution adjacent to syncytial germline nuclei at G1/S. During nutrient-restricted growth arrest, upregulated CKIa accumulated in arrested somatic endoreduplicative nuclei but did not do so in germline nuclei. In the latter context, Cyclin Dd levels gradually decreased. In contrast, the Cyclin Dbβ splice variant, lacking the Rb-interaction domain and phosphodegron, was specifically upregulated and the number of cytoplasmic foci containing this variant increased. This upregulation was dependent on stress response MAPK p38 signaling. We conclude that under favorable conditions, Cyclin Dbβ-CDK6 sequesters CKIa in the cytoplasm to cooperate with Cyclin Dd-CDK6 in promoting germline nuclear proliferation. Under nutrient-restriction, this sequestration function is enhanced to permit continued, though reduced, cycling of the germline during somatic growth arrest.
Co-expressed Cyclin D variants cooperate to regulate proliferation of germline nuclei in a syncytium
Creators: name: Gunasekaran Subramaniam
name: Campsteijn, Coen
name: Thompson, Eric M
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Cell Biology
subject: Molecular Biology
subject: Biotechnology
subject: Evolutionary Biology
subject: FOS: Biological sciences
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Developmental Biology
subject: Cancer
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: The role of the G1-phase Cyclin D-CDK 4/6 regulatory module in linking germline stem cell (GSC) proliferation to nutrition is evolutionarily variable. In invertebrate Drosophila and C. elegans GSC models, G1 is nearly absent and Cyclin E is expressed throughout the cell cycle, whereas vertebrate spermatogonial stem cells have a distinct G1 and Cyclin D1 plays an important role in GSC renewal. In the invertebrate, chordate, Oikopleura, where germline nuclei proliferate asynchronously in a syncytium, we show a distinct G1-phase in which 2 Cyclin D variants are co-expressed. Cyclin Dd, present in both somatic endocycling cells and the germline, localized to germline nuclei during G1 before declining at G1/S. Cyclin Db, restricted to the germline, remained cytoplasmic, co-localizing in foci with the Cyclin-dependent Kinase Inhibitor, CKIa. These foci showed a preferential spatial distribution adjacent to syncytial germline nuclei at G1/S. During nutrient-restricted growth arrest, upregulated CKIa accumulated in arrested somatic endoreduplicative nuclei but did not do so in germline nuclei. In the latter context, Cyclin Dd levels gradually decreased. In contrast, the Cyclin Dbβ splice variant, lacking the Rb-interaction domain and phosphodegron, was specifically upregulated and the number of cytoplasmic foci containing this variant increased. This upregulation was dependent on stress response MAPK p38 signaling. We conclude that under favorable conditions, Cyclin Dbβ-CDK6 sequesters CKIa in the cytoplasm to cooperate with Cyclin Dd-CDK6 in promoting germline nuclear proliferation. Under nutrient-restriction, this sequestration function is enhanced to permit continued, though reduced, cycling of the germline during somatic growth arrest.
Next Generation Sequencing Demonstrates Association between Tumor Suppressor Gene Aberrations and Poor Outcome in Patients with Cancer
Creators: name: Schwaederle, Maria
name: Daniels, Gregory A
name: Piccioni, David E
name: Kesari, Santosh
name: Fanta, Paul T
name: Schwab, Richard B
name: Shimabukuro, Kelly A
name: Parker, Barbara A
name: Razelle Kurzrock
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Chemistry
subject: Cancer
subject: Mathematics
subject: FOS: Mathematics
subject: Biological Sciences
subject: Molecular Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Cell Biology
subject: Medicine

description: ABSTRACTNext generation sequencing is transforming patient care by allowing physicians to customize and match treatment to their patients' tumor alterations. Our goal was to study the association between key molecular alterations and outcome parameters. We evaluated the characteristics and outcomes (overall survival (OS), time to metastasis/recurrence, and best progression-free survival (PFS)) of 392 patients for whom next generation sequencing (182 or 236 genes) had been performed. The Kaplan-Meier method and Cox regression models were used for our analysis, and results were subjected to internal validation using a resampling method (bootstrap analysis). In a multivariable analysis (Cox regression model), the parameters that were statistically associated with a poorer overall survival were the presence of metastases at diagnosis (P=0.014), gastrointestinal histology (P<0.0001), PTEN (P<0.0001), and CDKN2A alterations (P=0.0001). The variables associated with a shorter time to metastases/recurrence were gastrointestinal histology (P=0.004), APC (P=0.008), PTEN (P=0.026) and TP53 (P=0.044) alterations. TP53 (P=0.003) and PTEN (P=0.034) alterations were independent predictors of a shorter best PFS. A personalized treatment approach (matching the molecular aberration with a cognate targeted drug) also correlated with a longer best PFS (P=0.046). Our study demonstrated that, across diverse cancers, anomalies in specific tumor suppressor genes (PTEN, CDKN2A, APC, and/or TP53) were independently associated with a worse outcome, as reflected by time to metastases/recurrence, best PFS on treatment, and/or overall survival. These observations suggest that molecular diagnostic tests may provide important prognostic information in patients with cancer.
A K-band μ0-log h relation for early-type spiral galaxy disks
Creators: name: Graham, Alister
Publisher: name: Swinburne
Year: 2024

description: The K-band light-profiles from a diameter-limited sample of low-inclination (de Jong and van der Kruit, 1994) and high-inclination (Andredakis, Peletier, and Balcells, 1995) disk galaxies have been modelled with a seeing convolved Sersic r1/n bulge and an exponential disk. Rather than a constant central disk surface brightness, in the near-infrared μ0,K is shown to decrease with the logarithm of the disk scale-length (log h) – with a correlation coefficient equal to 0.78. Galaxy types Scd or later are observed not to partake in this relation.
Dynamische Gruppenarbeit - Dezentrale Feinplanungsunterstützung durch einen virtuellen Marktplatz
Creators: name: Münstermann, Thilo
name: Flachskampf, Paul
name: Völzke, Jens
Publisher: name: RWTH Aachen University
Year: 2010

description: Multikonferenz Wirtschaftsinformatik 2010 : Göttingen, 23. - 25. Februar 2010 ; Kurzfassungen der Beiträge / Matthias Schumann; Lutz M. Kolbe; Michael H. Breitner; Arne Frerichs (Hg.); Göttingen : Univ.-Verl. Göttingen 317-318 (2010).
description: Published by Univ.-Verl. Göttingen, Göttingen
Next generation sequencing demonstrates association between tumor suppressor gene aberrations and poor outcome in patients with cancer
Creators: name: Schwaederle, Maria
name: Daniels, Gregory A
name: Piccioni, David E
name: Kesari, Santosh
name: Fanta, Paul T
name: Schwab, Richard B
name: Shimabukuro, Kelly A
name: Parker, Barbara A
name: Razelle Kurzrock
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Chemistry
subject: Cancer
subject: Mathematics
subject: FOS: Mathematics
subject: Biological Sciences
subject: Molecular Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Cell Biology
subject: Medicine

description: Next generation sequencing is transforming patient care by allowing physicians to customize and match treatment to their patients’ tumor alterations. Our goal was to study the association between key molecular alterations and outcome parameters. We evaluated the characteristics and outcomes (overall survival (OS), time to metastasis/recurrence, and best progression-free survival (PFS)) of 392 patients for whom next generation sequencing (182 or 236 genes) had been performed. The Kaplan-Meier method and Cox regression models were used for our analysis, and results were subjected to internal validation using a resampling method (bootstrap analysis). In a multivariable analysis (Cox regression model), the parameters that were statistically associated with a poorer overall survival were the presence of metastases at diagnosis (P = 0.014), gastrointestinal histology (P < 0.0001), PTEN (P < 0.0001), and CDKN2A alterations (P = 0.0001). The variables associated with a shorter time to metastases/recurrence were gastrointestinal histology (P = 0.004), APC (P = 0.008), PTEN (P = 0.026) and TP53 (P = 0.044) alterations. TP53 (P = 0.003) and PTEN (P = 0.034) alterations were independent predictors of a shorter best PFS. A personalized treatment approach (matching the molecular aberration with a cognate targeted drug) also correlated with a longer best PFS (P = 0.046). Our study demonstrated that, across diverse cancers, anomalies in specific tumor suppressor genes (PTEN, CDKN2A, APC, and/or TP53) were independently associated with a worse outcome, as reflected by time to metastases/recurrence, best PFS on treatment, and/or overall survival. These observations suggest that molecular diagnostic tests may provide important prognostic information in patients with cancer.
Identity and parentage of two alpine grape cultivars from Switzerland (Vitis vinifera L. Lafnetscha and Himbertscha)
Creators: name: Vouillamoz, J. F.
name: Maigre, D.
name: Meredith, C. P.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Four closely related white grape cultivars from the Western Alps (Switzerland) - Humagne Blanc, Completer, Lafnetscha and Himbertscha - and three putative relatives or synonyms - Gouais Blanc, Plantscher and Bordeaux Blanc - were analyzed with up to 50 microsatellite markers. Humagne Blanc and Completer are ancient cultivars from the Haut-Valais and Graubünden regions, respectively. Lafnetscha and Himbertscha are lesser-known cultivars scarcely cultivated in Haut-Valais. Lafnetscha is frequently considered as synonym of Completer. Himbertscha might be related to Gouais Blanc, one of the parents of Chardonnay, Gamay, etc. Plantscher, a putative synonym of Lafnetscha, is scarcely cultivated in Haut-Valais (Switzerland) and Bordeaux Blanc (or Gros Bourgogne) is a cultivar of unknown origin (despite its names) cultivated in Switzerland. Our results allowed us to determine the true-to-type Lafnetscha and confirmed that Lafnetscha is not a synonym of Completer. Plantscher is not a synonym of Lafnetscha but a synonym of Bordeaux Blanc (or Gros Bourgogne) and is a likely parent or progeny of the Hungarian Furmint. Himbertscha is not related to Gouais Blanc and shares at least one allele at each locus with Humagne Blanc, providing strong evidence of a parent/progeny relationship. Given that Humagne Blanc is an older cultivar, we propose that it is the parent of Himbertscha. Alleles at 49 out of 50 microsatellite loci are consistent with Lafnetscha being the progeny of Completer and Humagne Blanc. The exception is a 10-base pair discrepancy at one locus (VVMD 36), most likely due to the occurrence of a null allele, since this parentage is supported at other markers by very high likelihood ratios. With Lafnetscha = Completer x Humagne Blanc, we present the second grape cultivar parentage showing a multiple repeat discrepancy at one locus. This study emphasizes that one multiple repeat unit discrepancy is not sufficient to reject a parentage, and that the greater is the number of loci, the greater are the chances to encounter null alleles or clonal mutations.
description: VITIS - Journal of Grapevine Research, Vol. 43 No. 2 (2004): Vitis
Status of yeast assimilable nitrogen in Italian grape musts and effects of variety, ripening and vintage
Creators: name: Nicolini, G.
name: Larcher, R.
name: Versini, G.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: The content of promptly assimilable nitrogen by yeast (PAN) was analysed in 586 juices from technologically ripe grapes collected in several Italian regions. A spectrophotometric method within the reach of each wine laboratory was used, adapting a previous method of the American Society of Brewing Chemists used for wort to grape juices. 58.3 % of the samples were below the classic deficiency threshold of 140 mg·l-1. Among varieties and vintages there were significant differences. An overall trend of PAN to slightly decrease with ripening was observed. The variability of the PAN content of numerous samples harvested in a vineyard to check ripeness seems to be larger than that for sugars and total acidity.
description: VITIS - Journal of Grapevine Research, Vol. 43 No. 2 (2004): Vitis
Physiological responses of native Tunisian grapevines and some rootstocks to direct iron deficiency
Creators: name: Ksouri, K.
name: Mahmoudi, R. H.
name: Gharsalli, M.
name: Lachaâl, M.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Research Note
description: VITIS - Journal of Grapevine Research, Vol. 43 No. 2 (2004): Vitis
title: Raumströmungsmessung mit Particle Image Velocimetry - Instationärer Betrieb von RLT-Anlagen
Creators: name: Mesenhöller, Eva
name: Vennemann, Peter
Publisher: name: FH Münster - University of Applied Sciences
Year: 2024

description: Überblick über Methodik, Hintergründe und Ziele des Projekts zu instationärer Raumlüftung sowie bisherige Untersuchungsschwerpunkte auf diesem Gebiet.
Galaxy light concentration. I. Index stability and the connection with galaxy structure, dynamics, and supermassive black holes
Creators: name: Graham, Alister
name: Trujillo, I.
name: Caon, N.
Publisher: name: Swinburne
Year: 2024

description: We explore the stability of different galaxy light concentration indices as a function of the outermost observed galaxy radius. With a series of analytical light-profile models, we show mathematically how varying the radial extent to which one measures a galaxy's light can strongly affect the derived galaxy concentration. The "mean concentration index," often used for parameterizing high-redshift galaxies, is shown to be horribly unstable, even when modeling one-component systems such as elliptical, dwarf elliptical, and pure exponential disk galaxies. The C31 concentration index performs considerably better but is also heavily dependent on the radial extent, and hence exposure depth, of any given galaxy. We show that the recently defined central concentration index is remarkably stable against changes to the outer radius and observational errors and provides a both meaningful and reliable estimate of galaxy concentration. The Sérsic index n from the r1/n models is shown to be monotonically related with the central concentration of light, giving the index n a second and perhaps more tangible meaning. With a sample of elliptical and dwarf elliptical galaxies, we present correlations between the central light concentration and the global parameters: luminosity (Pearson's r = -0.82), effective radius (r = 0.67), central surface brightness (r = -0.88), and velocity dispersion (r = 0.80). The more massive elliptical galaxies are shown to be more centrally concentrated. We speculate that the physical mechanism behind the recently observed correlation between the central velocity dispersion (mass) of a galaxy and the mass of its central supermassive black hole may be connected with the central galaxy concentration. That is, we hypothesize that it may not simply be the amount of mass in a galaxy but rather how that mass is distributed that controls the mass of the central black hole.
title: Raumströmungsmessung mit Particle Image Velocimetry (PIV) - Instationärer Betrieb von RLT-Anlagen
Creators: name: Mesenhöller, Eva
name: Vennemann, Peter
name: Hussong, Jeanette
Publisher: name: FH Münster - University of Applied Sciences
Year: 2024

description: Posterzusammenfassung der Hintergründe, Ziele, Messprinzipien, Messungen und des Versuchsstandes im Projekt zur Untersuchung instationärer Raumlüftung.
Seed and Plant Breeding for Wisconsin’s Organic Vegetable Sector: Understanding Farmers’ Needs
Creators: name: Lyon, Alexandra
name: Silva, Erin
name: Zystro, Jared
name: Bell, Michael
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Plant Biology
subject: FOS: Biological sciences
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: Developmental Biology
subject: Biological Sciences
subject: Ecology
subject: Earth and Environmental Sciences
subject: Biotechnology
subject: Microbiology

description: Plant breeding and seed issues are of growing importance in organic farming, but more regionally specific information is needed. In 2012, we surveyed Wisconsin organic vegetable growers regarding: a) general characteristics and farm practices; b) challenges in accessing organic seed; c) plant breeding priorities; and d) improving access to appropriate plant breeding and seed systems. Results suggest that growers had more difficulty accessing satisfactory genetics than seed quality, and that seed access was more problematic for growers with larger farming operations. Diverse plant breeding priorities, combined with prevalent variety trialing and seed saving, suggest a good fit with participatory plant breeding.
Supplementary_Materials_S3 from A standardized approach to quantifying activity in domestic dogs
Creators: name: Karimjee, Kamila
name: Harron, Rachel C. M.
name: Piercy, Richard J.
name: Daley, Monica A.
Publisher: name: The Royal Society
Year: 2024
Subjects: classificationCode: 320203
subject: Clinical microbiology
classificationCode: 400399
subject: Biomedical engineering not elsewhere classified

description: Objective assessment of activity via accelerometry can provide valuable insights into dog health and welfare. Common activity metrics involve using acceleration cut-points to group data into intensity categories and reporting the time spent in each category. Lack of consistency and transparency in cut-point derivation makes it difficult to compare findings between studies. We present an alternative metric for use in dogs: the acceleration threshold (as a fraction of standard gravity, 1 g = 9.81 m/s2) above which the animal's X most active minutes are accumulated (MXACC) over a 24-hour period. We report M2ACC, M30ACC and M60ACC data from a colony of healthy beagles (n = 6) aged 3–13 months. To ensure that reference values are applicable across a wider dog population, we incorporated labelled data from beagles and volunteer pet dogs (n = 16) of a variety of ages and breeds. The dogs' normal activity patterns were recorded at 200 Hz for 24-hours using collar-based Axivity-AX3 accelerometers. We calculated acceleration vector magnitude and MXACC metrics. Using labelled data from both beagles and pet dogs, we characterise the range of acceleration outputs exhibited enabling meaningful interpretation of MXACC. These metrics will help standardize measurement of canine activity and serve as outcome measures for veterinary and translational research.
Particle Classification by the Tandem Differential Mobility Analyzer–Particle Mass Analyzer System
Creators: name: Mikinori Kuwata
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: 60506 Virology
subject: FOS: Biological sciences
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Biotechnology
subject: Genetics
subject: Cell Biology
subject: Engineering
subject: Biochemistry

description: Particle mass analyzers, such as the aerosol particle mass analyzer (APM) and the Couette centrifugal particle mass analyzer (CPMA), are frequently combined with a differential mobility analyzer (DMA) to measure particle mass mp and effective density ρeff distributions of particles with a specific electrical mobility diameter dm. Combinations of these instruments, which are referred to as the DMA–APM or DMA–CPMA system, are also used to quantify the mass-mobility exponent Dm of non-spherical particles as well as to eliminate multiple charged particles. This study investigates the transfer functions of these setups, focusing especially on the DMA–APM system. The transfer function of the DMA–APM system was derived by multiplying the transfer functions of DMA and APM. The APM transfer function can be calculated using either the uniform or parabolic flow models. The uniform flow model provides an analytical function, while the parabolic flow model is more accurate. The resulting DMA–APM transfer functions were plotted on log(mp)-log(dp) space. A theoretical analysis of the DMA–APM transfer function demonstrated that the resolution of the setup is maintained when the rotation speed ω of APM is scanned to measure distribution. In addition, an equation was derived to numerically calculate the minimum values of the APM resolution parameter λc for eliminating multiple charged particles.Copyright 2015 American Association for Aerosol Research
Effectiveness of solitary and combined use of cover crop species to manage soilborne diseases in woody ornamental nursery production system
Creators: name: Parajuli, Madhav
name: Panth, Milan
name: Witcher, Anthony
name: Baysal-Gurel, Fulya
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Biotechnology
subject: Ecology
subject: FOS: Biological sciences
subject: Plant Biology

description: The understanding of how solitary and combined use of covercrop species and seeding rates influences soilborne diseases is lacking. Cover crops (crimson clover, triticale and crimson clover + triticale) were seeded in the field plots in September 2019 (Trial 1) and September 2021 (Trial 2) at two seeding rates (low and high) and a no cover cropped fallow served as a control. The soil was collected from all the plots in the following June. Red maple rooted cuttings were planted in nursery containers filled with those soils and plant health was evaluated with and without inoculation with Phytophthora nicotianae, Phytopythium vexans and Rhizoctonia solani. Plant height and fresh weight were measured, and plant roots were assessed for root rot severity (0–100% roots affected) and pathogen recovery. The number of fluorescent Pseudomonads present in soil samples was counted. Root rot severity and pathogen recovery were less with plants grown in cover cropped soil compared to non-cover cropped soil. Root rot severity caused by all three pathogens was least with the plants grown in the crimson clover and mixed cover cropped soil with the mixture being better than crimson clover. Moreover, plants grown in the high rate of cover cropped soil had less root rot severity compared to plants grown in low seeding rate cover cropped soil. Plant fresh weight was greater for plants grown in the high rate of crimson clover cover cropped soil and, in the mixture, compared to the non-cover cropped soil, whereas height was least affected. The Pseudomonad population was greatest in mixed cover cropped as well as in the high rate of cover cropped soil. Our findings suggest that a mixture of cover crop species and high seeding rate provides better service in suppressing root rot diseases likely associated with biological processes and nutrient availability.
Supplementary_Materials_S1 from A standardized approach to quantifying activity in domestic dogs
Creators: name: Karimjee, Kamila
name: Harron, Rachel C. M.
name: Piercy, Richard J.
name: Daley, Monica A.
Publisher: name: The Royal Society
Year: 2024
Subjects: classificationCode: 320203
subject: Clinical microbiology
classificationCode: 400399
subject: Biomedical engineering not elsewhere classified

description: Objective assessment of activity via accelerometry can provide valuable insights into dog health and welfare. Common activity metrics involve using acceleration cut-points to group data into intensity categories and reporting the time spent in each category. Lack of consistency and transparency in cut-point derivation makes it difficult to compare findings between studies. We present an alternative metric for use in dogs: the acceleration threshold (as a fraction of standard gravity, 1 g = 9.81 m/s2) above which the animal's X most active minutes are accumulated (MXACC) over a 24-hour period. We report M2ACC, M30ACC and M60ACC data from a colony of healthy beagles (n = 6) aged 3–13 months. To ensure that reference values are applicable across a wider dog population, we incorporated labelled data from beagles and volunteer pet dogs (n = 16) of a variety of ages and breeds. The dogs' normal activity patterns were recorded at 200 Hz for 24-hours using collar-based Axivity-AX3 accelerometers. We calculated acceleration vector magnitude and MXACC metrics. Using labelled data from both beagles and pet dogs, we characterise the range of acceleration outputs exhibited enabling meaningful interpretation of MXACC. These metrics will help standardize measurement of canine activity and serve as outcome measures for veterinary and translational research.
Symptomology, prevalence, and impact of Hop latent viroid on greenhouse-grown cannabis (Cannabis sativa L.) plants in Canada
Creators: name: Punja, Zamir K.
name: Wang, Keri
name: Lung, Samantha
name: Buirs, Liam
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Medicine
subject: Biotechnology
subject: Ecology
subject: FOS: Biological sciences
subject: Developmental Biology
subject: Mental Health
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Plant Biology
subject: Virology
subject: Computational Biology

description: The incidence of Hop latent viroid (HLVd) affecting cannabis plants in licenced production facilities in Canada during 2020–2023 was determined by RT-PCR analyses of 15 947 samples from nine provinces. Positive detection ranged from 5.3% to 92% of samples submitted, depending on province and year of sampling. The average country-wide HLVd infection incidence was 25.6%. Symptoms on affected plants varied with plant growth stage, and ranged from asymptomatic to mild leaf curl and mottling on stock (mother) plants and on vegetative plants, to extreme stunting and reduced inflorescence development on flowering plants. All symptomatic and some asymptomatic plants contained a 256 nucleotide RNA fragment with 100% sequence homology to HLVd from GenBank. The viroid was detected in various tissues of stock, vegetative, and flowering plants, including the uppermost leaves, at middle and lower positions in the canopy, and in the roots. The incidence of infection varied with the cannabis genotype. Flowering plants displayed yellowing or darkening of inflorescence leaves surrounding the pistillate flowers. Reductions of 12–42% in inflorescence stem lengths, fresh weights, and plant heights were observed in infected plants compared to noninfected plants, depending on the genotype. Levels of tetrahydrocannabinol (THC) and terpenes in diseased inflorescences were also significantly lower. The development of glandular trichomes which produce and store cannabinoids and terpenes was greatly reduced by HLVd infection. The affected trichomes had shorter stalk lengths and smaller glandular head sizes, and appeared shrivelled upon drying of the inflorescences. Hop latent viroid poses a significant economic threat to the cannabis industry.
Supplementary_Materials_S5 from A standardized approach to quantifying activity in domestic dogs
Creators: name: Karimjee, Kamila
name: Harron, Rachel C. M.
name: Piercy, Richard J.
name: Daley, Monica A.
Publisher: name: The Royal Society
Year: 2024
Subjects: classificationCode: 320203
subject: Clinical microbiology
classificationCode: 400399
subject: Biomedical engineering not elsewhere classified

description: Objective assessment of activity via accelerometry can provide valuable insights into dog health and welfare. Common activity metrics involve using acceleration cut-points to group data into intensity categories and reporting the time spent in each category. Lack of consistency and transparency in cut-point derivation makes it difficult to compare findings between studies. We present an alternative metric for use in dogs: the acceleration threshold (as a fraction of standard gravity, 1 g = 9.81 m/s2) above which the animal's X most active minutes are accumulated (MXACC) over a 24-hour period. We report M2ACC, M30ACC and M60ACC data from a colony of healthy beagles (n = 6) aged 3–13 months. To ensure that reference values are applicable across a wider dog population, we incorporated labelled data from beagles and volunteer pet dogs (n = 16) of a variety of ages and breeds. The dogs' normal activity patterns were recorded at 200 Hz for 24-hours using collar-based Axivity-AX3 accelerometers. We calculated acceleration vector magnitude and MXACC metrics. Using labelled data from both beagles and pet dogs, we characterise the range of acceleration outputs exhibited enabling meaningful interpretation of MXACC. These metrics will help standardize measurement of canine activity and serve as outcome measures for veterinary and translational research.
Effects of ripening stages and of plant vegetative vigor on the phenolic composition of grapes (Vitis vinifera L.) cv. Cabernet Sauvignon in the Maipo Valley (Chile)
Creators: name: Peña-Neira, A.
name: Dueñas, M.
name: Duarte, A.
name: Hernandez, T.
name: Estrella, I.
name: Loyola, E.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Quantitative changes in the composition of phenolic compounds in skins and seeds were determined during ripening of grape of Cabernet Sauvignon vines growing with low, medium or high vigor. Compounds in the skins were gallic and syringic acid, (+)-catechin, (-)-epicatechin, quercetin-3-galactoside, quercetin-3-rutinoside, quercetin-3-arabinglucoside, quercetin-3-glucoside, and quercetin-3-rhamnoside, kaempferol-3-rutinoside and kaempferol-3-glucoside. The following compounds were identified in seeds: gallic acid, (+)-catechin, (-)-epicatechin, and procyanidins B1, B2, B3 and B4. The composition of compounds depended on the stage of ripening and vigor. No clear relationship was found between groups of compounds.
description: VITIS - Journal of Grapevine Research, Vol. 43 No. 2 (2004): Vitis
Detailed workings for Equation 5.1 from Bounds on the spreading radius in droplet impact: the inviscid case
Creators: name: Amirfazli, Alidad
name: Bustamante, Miguel D.
name: Hu, Yating
name: Ó Náraigh, Lennon
Publisher: name: The Royal Society
Year: 2024
Subjects: classificationCode: 401299
subject: Fluid mechanics and thermal engineering not elsewhere classified
classificationCode: 490303
subject: Numerical solution of differential and integral equations
classificationCode: 490409
subject: Ordinary differential equations, difference equations and dynamical systems
classificationCode: 490410
subject: Partial differential equations
classificationCode: 499999
subject: Other mathematical sciences not elsewhere classified

description: A detailed description of the key steps in the derivation of Equation 5.1
Supplementary_Materials_S4 from A standardized approach to quantifying activity in domestic dogs
Creators: name: Karimjee, Kamila
name: Harron, Rachel C. M.
name: Piercy, Richard J.
name: Daley, Monica A.
Publisher: name: The Royal Society
Year: 2024
Subjects: classificationCode: 320203
subject: Clinical microbiology
classificationCode: 400399
subject: Biomedical engineering not elsewhere classified

description: Objective assessment of activity via accelerometry can provide valuable insights into dog health and welfare. Common activity metrics involve using acceleration cut-points to group data into intensity categories and reporting the time spent in each category. Lack of consistency and transparency in cut-point derivation makes it difficult to compare findings between studies. We present an alternative metric for use in dogs: the acceleration threshold (as a fraction of standard gravity, 1 g = 9.81 m/s2) above which the animal's X most active minutes are accumulated (MXACC) over a 24-hour period. We report M2ACC, M30ACC and M60ACC data from a colony of healthy beagles (n = 6) aged 3–13 months. To ensure that reference values are applicable across a wider dog population, we incorporated labelled data from beagles and volunteer pet dogs (n = 16) of a variety of ages and breeds. The dogs' normal activity patterns were recorded at 200 Hz for 24-hours using collar-based Axivity-AX3 accelerometers. We calculated acceleration vector magnitude and MXACC metrics. Using labelled data from both beagles and pet dogs, we characterise the range of acceleration outputs exhibited enabling meaningful interpretation of MXACC. These metrics will help standardize measurement of canine activity and serve as outcome measures for veterinary and translational research.
Particle Classification by the Tandem Differential Mobility Analyzer – Particle Mass Analyzer System
Creators: name: Mikinori Kuwata
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: 60506 Virology
subject: FOS: Biological sciences
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Biotechnology
subject: Genetics
subject: Cell Biology
subject: Engineering
subject: Biochemistry

description: Particle mass analyzers, such as the aerosol particle mass analyzer (APM) and the Couette centrifugal particle mass analyzer (CPMA), are frequently combined with a differential mobility analyzer (DMA) to measure particle mass mp and effective density ρeff distributions of particles with a specific electrical mobility diameter dm. Combinations of these instruments, which are referred as the DMA-APM or DMA-CPMA system, are also used to quantify the mass-mobility exponent Dm of non-spherical particles, as well as to eliminate multiply charged particles. This study investigates the transfer functions of these setups, focusing especially on the DMA-APM system. The transfer function of the DMA-APM system was derived by multiplying the transfer functions of the DMA and APM. The APM transfer function can be calculated using either the uniform or parabolic flow models. The uniform flow model provides an analytical function, while the parabolic flow model is more accurate. The resulting DMA-APM transfer functions were plotted on log(mp)− log(dp) space. A theoretical analysis of the DMA-APM transfer function demonstrated that the resolution of the setup is maintained when the rotation speed ω of the APM is scanned to measure distribution. In addition, an equation was derived to numerically calculate the minimum values of the APM resolution parameter λc for eliminating multiply charged particles.
Ripening-related gene expression during fruit ripening in Vitis vinifera L. cv. Cabernet Sauvignon and Clairette blanche
Creators: name: Burger, A. L.
name: Botha, F. C.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: The gene expression patterns in ripening fruit of a high quality wine cultivar (Cabernet Sauvignon) and a poor quality wine cultivar (Clairette blanche) were studied using cDNA-AFLP fingerprinting. Total RNA from "immature" (14-weeks post flowering) and "mature" (18-weeks post flowering) berries were used to study ripening-related gene expression in post-véraison stages of berry development. A total of 1,276 fragments were analysed, of which 175 appeared to be ripening-related. Average pairwise differences of the fragments amplified from "immature" and "mature" Cabernet Sauvignon and Clairette blanche berries, revealed a high level of similarity between the two cultivars. 70 % of the ripening-related fragments were cultivar-specific. The number of cultivar-specific and/or ripening-related fragments amplified, depended on the selective nucleotides of the primers used in the cDNA-AFLP analysis. Reverse slot blot and northern blot analysis confirmed that the expression of the identified genes were ripening-related.
description: VITIS - Journal of Grapevine Research, Vol. 43 No. 2 (2004): Vitis
Species diversity of Fusarium head blight and deoxynivalenol (DON) levels in Western Canadian wheat fields
Creators: name: Kannangara, S. K.
name: Bullock, P.
name: Walkowiak, S.
name: Fernando, W. G. D.
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Biochemistry
subject: Environmental Sciences not elsewhere classified
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: FOS: Biological sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Science Policy
subject: Plant Biology

description: Fusarium head blight (FHB) is primarily caused by Fusarium graminearum and is the most destructive wheat disease in western Canada. The trichothecene toxin deoxynivalenol (DON) and its derivatives 3-ADON and 15-ADON, which are produced by Fusarium, are toxic to humans and livestock. This study aimed to evaluate the Fusarium species and trichothecene-producing genotypes present in producer wheat fields of western Canada in 2019 and 2020. The grain analysis for DON showed that samples collected from both years experienced mild levels of FHB toxin contamination. In spring wheat, the highest mean DON content was recorded from Saskatchewan samples (0.9 ppm) in 2019 and Manitoba samples (0.3 ppm) in 2020. Winter wheat samples had lesser mean DON levels (<0.2 ppm) for all three provinces in both years. The chaff collected from the same spring wheat heads showed a significantly higher DON content than the grain in both years (P < 0.1 for 2019 and P < 0.001 for 2020), suggesting that chaff should be tested for DON before use as livestock feed. Most samples collected were infected by F. graminearum, with the highest F. graminearum percentage observed in the spring wheat from Manitoba. Further, F. avenaceum, F. sporotrichoides, F. culmorum, F. poae and F. acuminatum were found in infected samples. Most of the F. graminearum isolates were of the 3ADON genotype, which is the dominant genotype in FHB disease in western Canada. The study indicated that 3ADON-producing F. graminearum is becoming more frequent in western Canadian fields than the 15ADON genotype.
Anthesis, pollination and fruitset in Pinot Noir
Creators: name: Heazlewood, J. E.
name: Wilson, S.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Aspects of pollination and resulting fruitset in Vitis vinifera cv. Pinot Noir were investigated in a cool climate wine area of Southern Tasmania (Australia). Changes in the appearance of the stigmatic surface and pollen grains were recorded using environmental scanning electron microscopy (ESEM). Flowers with the calyptra intact (before capfall), showed an apparently turgid stigmatic surface and pollen grains present on the surface were slender and elongated (L/D 35 μm/15 μm). Stigmas had a more flaccid appearance after capfall and pollen grains were more spherical and less elongated in shape (L/D 28 μm/20 μm). Pollen was visible on the stigma surface before capfall, indicating that anthesis occurred whilst the calyptra was in place. Pollen viability tests showed that the pollen was already viable at this stage, and it remained viable until after the flower had been open for several days. Fluorescence micrographs showed no evidence of pollen tube growth until after capfall. Flowers covered by waxed paper bags to eliminate external pollen and thus allow only self-pollination, gave a percentage fruitset equal to that of flowers where external pollen was not excluded. Results indicate that Pinot Noir can be self-pollinated, and that while anthesis commences prior to capfall, fertilisation does not proceed until after capfall.
description: VITIS - Journal of Grapevine Research, Vol. 43 No. 2 (2004): Vitis
Sequence analysis of a microsatellite and its flanking regions in intraspecific hybrids of grapevine (Vitis vinifera L.)
Creators: name: Masi, E.
name: Vignani, R.
name: Sensi, E.
name: Scali, M.
name: Cresti, M.
name: Biselli, M.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Microsatellite (MS) VVMD21 (BOWERS et al. 1999) was taken as a model to explore the molecular basis of polymorphism in a panel of 6 grapevine accessions (Vitis vinifera L.), consisting of Sangiovese and Cabernet Sauvignon and 4 F1 plants derived from crossing both varieties. The 12 alleles of both parents and the progeny were cloned and sequenced. The microsatellite repeat (AG)n>6 was found in each sequence, together with a poly-T rich region that showed irregularity. Furthermore, single nucleotide deletion or exchange (point mutations) were found in the microsatellite flanking regions.
description: VITIS - Journal of Grapevine Research, Vol. 43 No. 2 (2004): Vitis
Evaluation of crown gall resistance in Vitis vinifera and hybrids of Vitis spp.
Creators: name: Mahmoodzadeh, H.
name: Nazemieh, A.
name: Majidi, I.
name: Paygami, I.
name: Khalighi, A.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Relative levels of crown gall susceptibility were determined in 17 genotypes of Vitis spp. by inoculating a diverse set of Agrobacterium vitis strains, measuring gall size and weight, and percentage of inoculated sites with galls. Hybrids of Vitis vinifera cv. Jighjigha x Vitis riparia “Gloire” (NAZ4) and V.vinifera cv. Alibaba x 110 R (NAZ5) were the most resistant genotypes but not completely immune. No genotype of V. vinifera was immune to crown gall. The interactions among strain and genotype were significant. V. vinifera cv. White Bidaneh was especially sensitive to the limited host range strain AG57. Weight and size of galls that were induced by 4 strains of Agrobacterium vitis were not significantly different for all genotypes of Vitis spp. But susceptibility of the genotypes to individual strains of A. vitis were significantly different.
description: VITIS - Journal of Grapevine Research, Vol. 43 No. 2 (2004): Vitis
Preuređenje kuće Domvs Francisci u Omišu iza 1861. godine
Creators: name: Savà, Giuseppe
Publisher: name: Zenodo
Year: 2020
Subjects: Domvs Farncisci
Kuća
Omiš
Nacrt
Celeghm

description: Usporedbom nacrta moguće je, uz ostale nalaze sagledati arhitektonsko stanje u kojem se kuća nalazila e u tom razdoblju. Kuća je prema sadržaju kamenog natpisa sa pročelja nastala 1625. godine, današnje arhitektonsko stanje i dispozicija prostorija ne odgovara ranijem ni izvornom, što osim ostalih nalaza potvrđuje i nacrt iz 1861. godine.
description: {"references": ["Herkov, Z. (1974). Prinosi za upoznavanje na\u0161ih starih mjera za du\u017einu i povr\u0161inu. Zbornik Odsjeka za povijesne znanosti Zavoda za povijesne i dru\u0161tvene znanosti Hrvatske akademije znanosti i umjetnosti, 7, 61-151."]}