title: ÐÐ·ÑÑÐµÐ½Ð¸Ðµ Ð¼ÐµÑ Ð°Ð½Ð¸Ð·Ð¼Ð° Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ð³ÐµÐ½Ð¾Ð² IES5 Ð¸ RAD2 Ð² Saccharomyces cerevisiae
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Genetic interaction of IES5 and RAD2 genes in Saccharomyces cerevisiae
Creators: name: ÐÐ¾Ð»Ð³Ð°Ð½Ð¾Ð²Ð°, ÐÐ½Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Ð£Ð¤-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¹ Ð¼ÑÑÐ°Ð³ÐµÐ½ÐµÐ·
ÑÐµÐ¼Ð¾Ð´ÑÐ»ÑÑÐ¸Ñ Ñ ÑÐ¾Ð¼Ð°ÑÐ¸Ð½Ð°
Saccharomyces cerevisiae
Ð³ÐµÐ½ IES5
Ð³ÐµÐ½ RAD2
UV-induced mutagenesis
chromatin remodulation
IES5 gene
RAD2 gene

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«ÐÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ðµ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ðµ Ð³ÐµÐ½Ð¾Ð²Â IES5Â Ð¸Â RAD2Â Ð²Â Saccharomyces cerevisiaeÂ». Ð ÑÐ°Ð±Ð¾ÑÐµ Ð¸Ð·ÑÑÐ°Ð»Ð¾ÑÑ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ ÑÐ°Ð±Ð¾ÑÑ Ð³ÐµÐ½Ð°Â IES5Â Ð² ÑÐµÐ¼Ð¾Ð´ÑÐ»Ð¸ÑÑÑÑÐµÐ¼ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐµÂ INO80 Ð½Ð° Ð²ÑÐ¶Ð¸Ð²Ð°ÐµÐ¼Ð¾ÑÑÑ Ð¸ Ð£Ð¤-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¹ Ð¼ÑÑÐ°Ð³ÐµÐ½ÐµÐ· Ð² ÐºÐ»ÐµÑÐºÐ°ÑÂ Saccharomyces cerevisiaeÂ Ð¸ ÐµÐ³Ð¾ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ðµ Ñ Ð³ÐµÐ½Ð¾Ð¼Â RAD2. Ð ÐµÑÐ°ÐµÐ¼ÑÐµ Ð·Ð°Ð´Ð°ÑÐ¸ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: 1) Ð¿Ð¾Ð´Ð¾Ð±ÑÐ°ÑÑ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ ÐºÐ°Ð½Ð°Ð²Ð°Ð½Ð¸Ð½Ð° Ð¸ Ð£Ð¤-Ð´Ð¾Ð·Ñ Ð´Ð»Ñ ÑÑÐ°Ð¼Ð¼Ð¾Ð²Â ies5Î,Â rad2ÎÂ Ð¸Â ies5Îrad2Î; 2) Ð¿Ð¾ÑÑÐ°Ð²Ð¸ÑÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÑ Ð½Ð° Ð²ÑÐ¶Ð¸Ð²Ð°ÐµÐ¼Ð¾ÑÑÑ Ð¸ ÑÐ°ÑÑÐ¾ÑÑ Ð¼ÑÑÐ°Ð³ÐµÐ½ÐµÐ·Ð° Ñ Ð²ÑÑÐµÐ¿ÐµÑÐµÑÐ¸ÑÐ»ÐµÐ½Ð½ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð²; 3) Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐ°ÑÑ Ð¸ ÑÑÐ°Ð²Ð½Ð¸ÑÑ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ; 4) Ð¾ÑÐµÐ½Ð¸ÑÑ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð¼ÑÑÐ°ÑÐ¸Ð¸ Ð² Ð³ÐµÐ½ÐµÂ IES5,Â ÐºÐ¾Ð´Ð¸ÑÑÑÑÐµÐ³Ð¾ Ð¾Ð´Ð½Ñ Ð¸Ð· ÑÑÐ±ÑÐµÐ´Ð¸Ð½Ð¸Ñ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ°Â INO80, Ð½Ð° Ð£Ð¤-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¹ Ð¼ÑÑÐ°Ð³ÐµÐ½ÐµÐ·. ÐÐ±ÑÐµÐºÑÐ¾Ð¼ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐ²Ð»ÑÐ»Ð¸ÑÑ Ð´ÑÐ¾Ð¶Ð¶Ð¸Â Saccharomyces cerevisiaeÂ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð²Â ies5Î,Â rad2ÎÂ Ð¸Â ies5Îrad2Î. Ð Ð°Ð±Ð¾ÑÐ° Ð²ÑÐ¿Ð¾Ð»Ð½ÑÐ»Ð°ÑÑ Ð½Ð° Ð±Ð°Ð·ÐµÂ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ Ð³ÐµÐ½ÐµÑÐ¸ÐºÐ¸ ÑÑÐºÐ°ÑÐ¸Ð¾Ñ Ð¾ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾Ð¹ Ð¸ ÑÐ°Ð´Ð¸Ð°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ Ð±Ð¸Ð¾ÑÐ¸Ð·Ð¸ÐºÐ¸ ÐÐÐ¦ Â«ÐÑÑÑÐ°ÑÐ¾Ð²ÑÐºÐ¸Ð¹ Ð¸Ð½ÑÑÐ¸ÑÑÑ â ÐÐÐ¯Ð¤Â». ÐÐ½Ð°Ð»Ð¸Ð·Ñ Ð¿Ð¾Ð´Ð²ÐµÑÐ³Ð°Ð»Ð¸ÑÑ Ð¿Ð¾ÐºÐ°Ð·Ð°ÑÐµÐ»Ð¸ Ð²ÑÐ¶Ð¸Ð²Ð°ÐµÐ¼Ð¾ÑÑÐ¸ Ð¸ ÑÐ°ÑÑÐ¾ÑÑ Ð£Ð¤-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð¼ÑÑÐ°Ð³ÐµÐ½ÐµÐ·Ð° ÑÑÐµÑ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð². ÐÐ¾ Ð¸ÑÐ¾Ð³Ñ Ð±ÑÐ» ÑÐ´ÐµÐ»Ð°Ð½ Ð²ÑÐ²Ð¾Ð´, ÑÑÐ¾ Ð¼ÑÑÐ°ÑÐ¸Ñ Ð¿Ð¾ Ð³ÐµÐ½ÑÂ IES5Â Ð¿ÑÐ¸Ð²Ð¾Ð´Ð¸Ñ Ðº Ð¿Ð¾Ð²ÑÑÐµÐ½Ð¸Ñ ÑÐ°ÑÑÐ¾ÑÑ Ð¼ÑÑÐ°Ð³ÐµÐ½ÐµÐ·Ð°, ÑÐ°ÐºÐ¶Ðµ Ð¾Ð½Ð° ÑÐ¿Ð¸ÑÑÐ°ÑÐ¸ÑÑÐµÑ Ðº Ð´ÐµÐ»ÐµÑÐ¸Ð¸Â RAD2, Ð¸ Ð¾ÑÐµÐ½ÐµÐ½Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ ÑÐ°Ð±Ð¾ÑÑ ÑÐµÐ¼Ð¾Ð´ÑÐ»Ð¸ÑÑÑÑÐµÐ³Ð¾ ÑÑÐ¾Ð¼Ð°ÑÐ¸Ð½ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ°Â INO80.
description: Topic of the final qualification work: "Genetic interaction ofÂ IES5Â andÂ RAD2Â genes in Saccharomyces cerevisiae". We studied the effect of theÂ IES5Â gene in the INO80 remodulatory complex on survival and UV-induced mutagenesis inÂ Saccharomyces cerevisiaeÂ cells and its interaction with theÂ RAD2Â gene.Tasks to be solved during the research: 1) select canavanin concentrations and UV doses for strainsÂ ies5Î,Â rad2ÎÂ andÂ ies5Îrad2Î; 2) to conduct experiments on the survival and frequency of mutagenesis in the above strains; 3) process and compare the obtained results; 4) to evaluate the effect of a mutation in theÂ IES5Â gene encoding one of the subunits of the INO80 complex on UV-induced mutagenesis. The object of the study was the yeast Saccharomyces cerevisiae of various strainsÂ ies5Î,Â rad2ÎandÂ ies5Îrad2Î. The work was carried out based on the Eukaryote Genetics Laboratory of the PNPI named by B. P. Konstantinov of NRC Â«Kurchatov InstituteÂ». The survival rates and frequency of UV-induced mutagenesis of three different strains were analyzed. As a result, it was concluded that the mutation in theÂ IES5Â gene leads to an increase in the frequency of mutagenesis, it also epistatizes to the deletion ofÂ RAD2, and the influence of the work of the INO80 chromatin remodulating complex was evaluated.