cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Assessment of acute and subacute toxicity, pharmacokinetics, and biodistribution of eugenol nanoparticles after oral exposure in Wistar rats
Creators: name: Nagaraju, Pramod G.
name: S., Ashwini
name: Rao, Pooja J.
name: Priyadarshini, Poornima
Publisher: name: Taylor & Francis
Year: 2024
Subjects: subject: Biochemistry
subject: Space Science
subject: Medicine
subject: Cell Biology
subject: Pharmacology
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: FOS: Biological sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified

description: The present study aimed to assess the safety, toxicity, biodistribution, and pharmacokinetics of eugenol nanoparticles (EONs) following oral administration in Wistar rat models. In the acute toxicity study, the rats were given a fixed dose of 50, 300, and 2000 mg/kg body weight per group orally and screened for 2 weeks after administration. In the subacute study, three different doses (500, 1000, and 2000 mg/kg BW) of EON were administered for 28 days. The results indicated no significant differences in food and water consumption, bodyweight change, hematological and biochemical parameters, relative organ weights, gross findings, or histopathology compared to the control. Additionally, no significant changes were observed in the expression profiles of inflammatory cytokines such as IL-1, IL-6, and TNFα in the plasma, confirming the absence of systemic inflammation. Biodistribution analysis revealed rapid absorption of eugenol and improved bioavailability due to gradual and sustained release, leading to a maximum eugenol concentration of 15.05 μg/mL (Cmax) at approximately 8 h (Tmax) in the blood plasma. Thus, the study provides valuable insights into the utilization of EON for enhancing the stability, solubility, and sustained release of eugenol and highlights its promising safety profile in vivo.
The AO Pediatric Comprehensive Classification of Long Bone Fractures (PCCF) – Part I:
Creators: name: Joeris, Alexander
name: Lutz, Nicolas
name: Blumenthal, Andrea
name: Slongo, Theddy
name: Audigé, Laurent
Publisher: name: Taylor & Francis
Year: 2016
Subjects: subject: Biochemistry
subject: 29999 Physical Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Physiology
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics
subject: Developmental Biology
subject: Science Policy
subject: Hematology

description: Background and purpose — To achieve a common understanding when dealing with long bone fractures in children, the AO Pediatric Comprehensive Classification of Long Bone Fractures (AO PCCF) was introduced in 2007. As part of its final validation, we present the most relevant fracture patterns in the upper extremities of a representative population of children classified according to the PCCF. Patients and methods — We included children and adolescents (0–17 years old) diagnosed with 1 or more long bone fractures between January 2009 and December 2011 at the university hospitals in Bern and Lausanne (Switzerland). Patient charts were retrospectively reviewed and fractures were classified from standard radiographs. Results — Of 2,292 upper extremity fractures in 2,203 children and adolescents, 26% involved the humerus and 74% involved the forearm. In the humerus, 61%, and in the forearm, 80% of single distal fractures involved the metaphysis. In adolescents, single humerus fractures were more often epiphyseal and diaphyseal fractures, and among adolescents radius fractures were more often epiphyseal fractures than in other age groups. 47% of combined forearm fractures were distal metaphyseal fractures. Only 0.7% of fractures could not be classified within 1 of the child-specific fracture patterns. Of the single epiphyseal fractures, 49% were Salter-Harris type-II (SH II) fractures; of these, 94% occurred in schoolchildren and adolescents. Of the metaphyseal fractures, 58% showed an incomplete fracture pattern. 89% of incomplete fractures affected the distal radius. Of the diaphyseal fractures, 32% were greenstick fractures. 24 Monteggia fractures occurred in pre-school children and schoolchildren, and 2 occurred in adolescents. Interpretation — The pattern of pediatric fractures in the upper extremity can be comprehensively described according to the PCCF. Prospective clinical studies are needed to determine its clinical relevance for treatment decisions and prognostication of outcome.
The AO Pediatric Comprehensive Classification of Long Bone Fractures (PCCF) – Part II:
Creators: name: Joeris, Alexander
name: Lutz, Nicolas
name: Blumenthal, Andrea
name: Slongo, Theddy
name: Audigé, Laurent
Publisher: name: Taylor & Francis
Year: 2016
Subjects: subject: Biochemistry
subject: Space Science
subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Physiology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Ecology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Science Policy
subject: Hematology

description: Background and purpose — To achieve a common understanding when dealing with long bone fractures in children, the AO Pediatric Comprehensive Classification of Long Bone Fractures (AO PCCF) was introduced in 2007. As part of its final validation, we present the most relevant fracture patterns in the lower extremities of a representative population of children classified according to the PCCF. Patients and methods — We included patients up to the age of 17 who were diagnosed with 1 or more long bone fractures between January 2009 and December 2011 at either of 2 tertiary care university hospitals in Switzerland. Patient charts were retrospectively reviewed. Results — More lower extremity fractures occurred in boys (62%, n = 341). Of 548 fractured long bones in the lower extremity, 25% involved the femur and 75% the lower leg. The older the patients, the more combined fractures of the tibia and fibula were sustained (adolescents: 50%, 61 of 123). Salter-Harris (SH) fracture patterns represented 66% of single epiphyseal fractures (83 of 126). Overall, 74 of the 83 SH patterns occurred in the distal epiphysis. Of all the metaphyseal fractures, 74 of 79 were classified as incomplete or complete. Complete oblique spiral fractures accounted for 57% of diaphyseal fractures (120 of 211). Of all fractures, 7% (40 of 548) were classified in the category "other", including 29 fractures that were identified as toddler’s fractures. 5 combined lower leg fractures were reported in the proximal metaphysis, 40 in the diaphysis, 26 in the distal metaphysis, and 8 in the distal epiphysis. Interpretation — The PCCF allows classification of lower extremity fracture patterns in the clinical setting. Re-introduction of a specific code for toddler’s fractures in the PCCF should be considered.
Using the duration of combined sewer overflow events for the calibration of sewer hydrodynamic models
Creators: name: A. Montserrat
name: T. Hofer
name: M. Poch
name: D. Muschalla
name: Ll. Corominas
Publisher: name: Taylor & Francis
Year: 2016
Subjects: subject: Space Science
subject: Medicine
subject: Neuroscience
subject: Physiology
subject: FOS: Biological sciences
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: 19999 Mathematical Sciences not elsewhere classified
subject: FOS: Mathematics
subject: Plant Biology

description: This paper compares two calibration approaches to enhance the ability of hydrodynamic models to describe the performance of combined sewer overflow (CSO) structures. One approach is based on the duration of CSO events (monitored by low-cost sensors) and the other focuses on the overflow volume. Both were applied to the West-Graz catchment model to simulate the discharge from the CSO overflow channel under small, medium and large rain episodes. The methodology includes sensitivity analysis of the model parameters and automatic calibration through optimization. The results revealed that the calibration using the CSO duration led to results similar to those of the approach that used overflow volume, with less than 12% error differences between approaches for medium and large rain episodes.
Binding interaction of a fluoranthene–thiol on gold nanoparticles with β-cyclodextrin and DNA
Creators: name: S. Eugene Thangaraj
name: Antony, Eva Janet
name: Sameena Yousuf
name: P. Mosae Selvakumar
name: Premnath Dhanaraj
name: Enoch, Israel V. M. V.
Publisher: name: Taylor & Francis
Year: 2016
Subjects: subject: Biophysics
subject: Biochemistry
subject: Space Science
subject: 29999 Physical Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: Medicine
subject: Molecular Biology
subject: Pharmacology
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Cancer

description: In this paper, we report the preparation of a fluoranthene–thiol derivative, the assembly of the compound onto gold nanoparticles, and their binding to β-cyclodextrin and DNA. The synthesised fluoranthene–thiol is characterised using infra-red (IR), nuclear magnetic resonance (NMR), and mass spectrometric techniques. The gold nanoparticles are characterised using transmission electron microscopy and energy dispersive X-ray spectroscopy microanalysis. The size of the nanoparticles is 5 ± 1 nm. The thiol–gold nanoparticles bind to β-cyclodextrin, and DNA and the fluorescence spectra show enhancement of fluorescence on the binding. The thiol–gold nanoparticles form a host–guest complex with β-cyclodextrin and the binding constant of the complex is 1330 mol−1 dm3. This reveals the openness of the thiol on the surface of the gold nanoparticles.
À balles réelles - Photographie documentaire et ethnographie réflexive - Tonnerre, 2017-2022
Creators: name: Dantou, Jean-Robert
Publisher: name: Zenodo
Year: 2024

description: Thèse de doctorat Sciences Arts Création Recherche déposée en mars 2024 à l'École normale supérieure - PSL. 

 

Avertissement

Il a été décidé pour cette recherche, en accord avec la direction scientifique et dans l’objectif de protéger la vie privée des personnes photographiées, de traiter de manière séparée deux types de productions : un tapuscrit illustré (le présent ouvrage) et une exposition qui se tiendra dans la ville de Tonnerre en juin 2024.

Dans le tapuscrit, des textes concernant la vie privée des personnes sont apportés à la connaissance du lecteur en même temps que les photographies. Les noms et prénoms des personnes sont anonymisés, y compris dans les légendes des photographies, mais les personnes étant reconnaissables, il a été décidé de ne rendre accessible ce document qu’au strict jury de thèse. Le tapuscrit illustré est donc strictement confidentiel.

Dans l’exposition prévue à l’Hôtel Cœurderoy de Tonnerre du 1er au 29 juin 2024, les légendes des photographies mentionnent les noms et prénoms des personnes sans dispositif d’anonymisation. Les textes et légendes accompagnant les photographies tiennent compte du caractère public de cette diffusion et font l’objet de validations en amont par les personnes photographiées elles-mêmes ainsi que par le comité scientifique de l’exposition.

Dans l’exposition comme dans la thèse, il a été décidé de ne pas anonymiser les noms de lieux.
Bordering and Debordering Across Time. Refugees and Asylum Seekers Facing Chronopolitics
Creators: name: Della Puppa, Francesco
name: Storato, Giulia
name: Sanò, Giuliana
Publisher: name: Italian Sociological Review
Year: 2024

description: With these contributes we investigate how refugees and asylum seekers deal with the politics of time that run within asylum policies, including the different production of temporal regimes along diverse types of migrant centres. By bringing together papers and case studies that map the trajectories of time undertaken by individuals in their daily routines and life experiences, we attempt to set up a reflection on the notion of a ‘landscape of time’. If, on the one hand, the interactions between spatial confinement and temporality of immigration controls realise multiple forms of (im)mobility of refugees and asylum seekers; on the other hand, the practices of routinisation, acceleration, stasis and waiting – exercised by individuals both inside and outside the centres and across the borders – can also be read as a tactic aimed at claiming time; a time which is differently experienced, according to the current system of social and civil stratification.

Therefore, moving within these premises, we focus on how the ‘chronopolitics’ of the asylum and reception system affect daily lives and biographical trajectories of refugees and asylum seekers, both during their experience of migration and within the so called ‘reception system’.
description: Italian Sociological Review, Vol. 14 No. 9S (2024)
Stuck but Not Immobile. Waiting, (Im)Mobility and Agency of Refugees and Asylum Seekers Along the Balkan Routes
Creators: name: Martini, Chiara
Publisher: name: Italian Sociological Review
Year: 2024

description: The Balkan route, or rather, the Balkan routes, which in recent years have been crossed by many different people, represent a paradigmatic territory on which practices of control, subjugation and precarisation are experimented to the detriment of those in transit. Their lives are punctuated by dynamics and structures that create a constant tension between mobility and immobility, between acceleration and waiting, with obvious and different consequences on trajectories, migration projects and agency. Greece and the Balkan countries represent two emblematic cases of what certain policies of control and closure can produce, both at a structural and individual level. Starting from a fieldwork carried out in Greece and Bosnia and Herzegovina from August to November 2021, this paper will try to analyze how forced waiting and immobility, imposed by the border regime and by asylum and reception systems, can in fact produce illegality (De Genova, 2004), uncertainty (Griffiths, 2013) and precariousness (Khosravi, 2017). Thanks to the collection of testimonies and stories related to the condition of people stranded in these countries, I will show how the hypermobility produced by these dynamics can also represent a tactic, an attempt to regain control over one’s migratory trajectories and to put an end to temporal and geographical stuckedness.
description: Italian Sociological Review, Vol. 14 No. 9S (2024)
The Temporalities of Non-deportability. Rejected Asylum-Seekers Trapped Between Labor Market Forces, Control, and Integration Policies
Creators: name: Fontanari, Elena
name: Dimitriadis, Iraklis
Publisher: name: Italian Sociological Review
Year: 2024

description: This paper focuses on the experiences of rejected asylum-seekers (RAS) caught in their everyday lives between deportation threat and mechanisms of irregularization. We analyze their everyday lives in Italy and Germany, two EU member states facing the non-deporability of RAS in different ways according to the specificity of their labour market forces, integration policies, and democratic institutional culture. Furthermore, this paper aims to focus on the temporality as a crucial dimension to grasp the power relations between the technologies implemented to govern migrants on the move, and the everyday struggles put in place to face and overcome the barriers raised to deter their integration. The dialogue between the two case-studies contributes to a better understanding on how post-arrival migration enforcement regimes and their different underlying rationales produce temporal regimes strongly affecting the everyday lives of RAS. From one side, we look at time as technology to govern population in different manner according to the different types of “migration enforcement regimes” (Leerkes and Van Houte, 2020). From the other side, we explore how RAS deal with the politics of time (Low, 2003) that run within asylum and deportation policies, and their (non)-implementation, while struggling to make their life into the host society.
description: Italian Sociological Review, Vol. 14 No. 9S (2024)
Visual trimorphemic compound recognition in a morphographic script
Creators: name: Miwa, Koji
name: Libben, Gary
name: Ikemoto, Yu
Publisher: name: Taylor & Francis
Year: 2016
Subjects: subject: Neuroscience
subject: Pharmacology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Science Policy

description: This lexical decision with eye-tracking study investigated how Japanese trimorphemic compounds (e.g. 体温計 “clinical thermometer”) are recognised. The questions answered were, in the course of decomposing and composing Japanese trimorphemic compounds, (1) whether recognition processes are tuned for a specific branching direction, (2) whether the morphological processing proceeds in a bottom-up combinatorial manner, and (3) whether the three constituents of trimorphemic compounds are equally important and processed serially. Mixed-effects regression analyses of response times and fixation durations revealed that a left-branching advantage appears in a late time frame and that, although there was early processing of the whole compound from the first fixation, a character frequency effect was also observed. Furthermore, the first and the third, but not the second, constituent frequencies contributed to compound recognition. This bathtub-like effect was further supported by corpus-based evidence: the conditional probability for the second constituent is incomparably high.
The Contested Mediterranean. Temporalities of New Forms of Migration Containment in Italy During the COVID-19 Pandemic
Creators: name: Caja, Emilio
Publisher: name: Italian Sociological Review
Year: 2024

description: With the outbreak of Covid-19, attention has been increasingly given to new forms of confinement, both in reception facilities and detention centres. In this context, emerging forms of immigration containment and detention have initially been analysed mostly through spatial lenses. In particular, in Italy, new forms of migration containment, the so-called ‘quarantine ships’, have been analysed mostly on their spatial features: in the middle of the sea, far away from the space of the “national polity”. Yet, throughout the two years of existence of quarantine ships, attention has been drawn to the temporal dimension of this new form of migration containment. In this sense, moving beyond the analysis of quarantine ships as a static and unique form of containment, and including the ships into a broader picture that takes into consideration what happens before and after them, the article aims at understanding how new spatio-temporal regimes of control affect asylum seekers’ lives. In particular, it studies how quarantine ships have become a central device of a specific temporal regime of migration control that developed with the outbreak of Covid-19; what the connections between this regime and pre-pandemic containment configurations are; and, finally, what tactics of resistance have been put in place by asylum seekers and those acting in solidarity with them. Quarantine ships and the temporal governance strategy that developed around them (the “detention-deportation chain”) can be better understood looking at the increasing logistification of asylum seeking. On the other hand, within this emerging form of government,  asylum seekers and those acting in solidarity with them put in place tactics of resistance to interrupt the suspended time imposed by this regime; to interrupt sudden accelerations, as in the case of deportations; and, finally, through practices of active memory, they challenged the status quo imposed by the EUropean border regime.
description: Italian Sociological Review, Vol. 14 No. 9S (2024)
Street-Level Workers and the Temporalities of Waiting in the Italian Asylum System
Creators: name: Pasian, Pamela
Publisher: name: Italian Sociological Review
Year: 2024

description: Immigration and asylum have become enormously contested in Italy over recent decades. Weak planning capacities and logics of emergency have typically permeated Italian policymaking on migration and, even more so, on asylum. Currently the Italian reception system implements a widespread framework managed by public authorities. It is characterized by policies of subsidiarity where different actors, public and private, are involved at various levels of government. Generally, public authorities subcontract third sector actors and NGOs to provide inclusion and integration services. The role of these agents is the focus of this article, particularly how they use their discretionary power to cope with the task of implementing a state mandate concerning the management of the condition of immobility that affects the temporality of refugees and asylum seekers, while waiting to obtain a permit or social and labour inclusion processes.

Drawing on ethnographic research realized in the realm of the project “SIforREF - Integrating Refugees in Society and Labour Market through Social Innovation”, founded by the Interreg Central Europe Program, this contribution shows how street-level workers use their discretionary power as a tactic that promotes their value and beliefs.
description: Italian Sociological Review, Vol. 14 No. 9S (2024)
Border Regimes, Shifting Temporalities and Migrant Responses. An Analysis of the Route Between the Eastern Mediterranean and the North-Western French-Italian Border
Creators: name: Montagna, Nicola
name: Gorza, Piero
name: Moschella, Rita
name: Perino, Maria
Publisher: name: Italian Sociological Review
Year: 2024

description: The North-western French-Italian border is an important cross point for people coming from the Balkans and the Central Mediterranean routes aiming to reach Northern-continental European countries and the United Kingdom. According to the data we collected, in 2021 some 10,369 people, including 400 unaccompanied minors and 412 families, arrived at the Alpine border – twice the 2020 numbers, when around 4,700 stopped at the two shelters. Although in 2022 the figure had dropped to about 8,500 it remains high, confirming this border as highly relevant. Based on data collected between 2021 and 2022 in Oulx, a village in the upper Susa Valley, North-western Italy, this article aims to investigate the impact of borders on migrants’ temporalities among people who cross the border with France wanting to reach Europe. Borders as a spatial mechanism for controlling people’s movement are bound up with time: the time of displacement in camps, the time migrants spend attempting to cross the borders, the overall time their movement takes, which is also affected by mobility policies. This does not happen in a vacuum of migrant agency. People on the move respond to the constraints of border regimes in a variety of ways, including resorting to smugglers and changing family and household figurations. However, as this article aims to show, the kinds of responses adopted are not always freely chosen, but they are forms of adaptation to the circumstances and constraints imposed by the border regime.
description: Italian Sociological Review, Vol. 14 No. 9S (2024)
Contested Time. Migrants’ Temporal Practices and Agency in Institutional Reception and Grassroots Solidarity at the Canary Islands
Creators: name: Giliberti, Luca
name: Fravega, Enrico
Publisher: name: Italian Sociological Review
Year: 2024

description: During the pandemic, due to the worsening of the already weak local economies of different Western African countries (Morocco, Western Sahara, Mauritania, Senegal), the impermeabilisation of the Ceuta/Melilla border and the consequent redefinition of the main migration routes, the Atlantic route towards the Canary Islands – which was relevant between the late 1990s and early 2000s – regained significance in undocumented migration towards Europe. The emergency management of this flow of people, which since 2020 has brought thousands of migrants to the shores of the Canary Islands, has continued for months and months, through improvised forms of reception in camps (Muelle de la verguenza) and empty hotels (due to the pandemic crisis), until the establishment of migrants’ emergency reception centres. Facing the often-inhuman conditions of these centres, as well as the situations of temporal injustice and the risk of deportation, many migrants refuse institutional relocation, leaving the reception system and dwelling in the streets. In this way, albeit in an ambivalent dimension, they break the frame on the public discourse concerning them as mere subjects in need of care and containment, regaining at least partial control of their time and opening up to a series of encounters with the emerging solidarity networks from below. This paper, based on an ethnographic fieldwork carried out, during 2022 and the beginning of 2023, on the island of Gran Canaria, means to explore the complex relation between temporality and migrants’ agency, with particular attention to forms of institutional reception and grassroots hospitality.
description: Italian Sociological Review, Vol. 14 No. 9S (2024)
Examining radical position and function in Chinese character recognition using the repetition blindness paradigm
Creators: name: Chen, Yi-Chuan
name: Yeh, Su-Ling
Publisher: name: Taylor & Francis
Year: 2016
Subjects: subject: Biophysics
subject: Medicine
subject: Neuroscience
subject: Physiology
subject: FOS: Biological sciences
subject: 111714 Mental Health
subject: FOS: Health sciences

description: Repetition blindness (RB) is the failure to report the second occurrence of repeated items in a rapid serial visual presentation stream. The two-stage model of RB by Bavelier (1994) states that more properties shared between the repeated items lead to a larger RB effect. We used RB paradigm to examine the position (left or right) and the function (semantic or phonetic) of radicals in Chinese character recognition. Compared to the repeated radicals with the same position and function, RB was reduced when they were in different positions (Experiment 1A), but not when they had different functions (Experiment 1B). Similar RB-effect was observed when only one, or both, of the repeated radicals provided valid semantic or phonetic cues to characters (Experiments 2A and 2B). These results suggest that radicals are encoded with position but not function information. The radical function is likely implemented in lateral connections between semantic and phonological representations of characters.
Lenz- und Liebeslieder : Ein Liederspiel für gemischten Chor, Solo und Klavier zu 4 Händen komponiert : Op. 72
Creators: name: Huber, Hans
Publisher: name: Siegel
Year: 1885

description: II. Gesangsmusik. 2. Für gem. Chor & Klavierbegl.
description: [Komponiert ca. 1884]
Compounds, competition, and incremental word identification in spoken Cantonese
Creators: name: Tsang, Cara
name: Chambers, Craig G.
name: Mozuraitis, Mindaugas
Publisher: name: Taylor & Francis
Year: 2016
Subjects: subject: Pharmacology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: 111714 Mental Health
subject: FOS: Health sciences

description: The majority of words in Cantonese are compounds, which seems likely to burden the process of identifying words in running speech. Cantonese is also a stress-timed language, which reduces the potential for durational contrasts to distinguish embedded constituents from self-standing words. The current study demonstrates the challenge of identifying words in spoken Cantonese. As a compound unfolds, listeners are more likely to consider an onset-embedded constituent as the intended word than the actual word they are hearing – a result that seems poorly adapted to the prevalence of compounds. However, the results also show these challenges are offset by sentence-based cues, such as those provided by noun classifiers. This occurs despite variability in classifier-noun pairings and the fact that adult speakers often show incomplete mastery of these pairings. Together the results demonstrate how even highly biased cases of lexical competition are overcome by sentence-level constraints that may be only moderate in strength.
Hif2-alpha stabilization in Interstitial Macrophages Causes Thrombospondin-1 Mediated Hypoxic Pulmonary Hypertension.
Creators: name: Kumar, Rahul
Publisher: name: Zenodo
Year: 2024

description: Thrombospondin-1 (TSP-1) mediated activation of TGF-β signaling is necessary and sufficient for pulmonary hypertension (PH). Hypoxia drives increased density of macrophages within the perivascular/adventitial space and molecular reprogramming. The origin and phenotype of pathologic TSP-1 producing macrophages in hypoxia PH are unclear. Transforming growth factor-b (TGF-b) signalling is required for chronic hypoxia-induced pulmonary hypertension (PH). The activation of TGF-b by thrombospondin-1 (TSP-1) contributes to the pathogenesis of hypoxia-induced PH. However, neither the cellular source of pathologic TSP-1 nor the downstream signalling pathway that link activated TGF-b to PH have been determined. In this study, we hypothesized that circulating monocytes, which are recruited to become interstitial macrophages (IMs), are the major source of TSP-1 in hypoxia-exposed mice, and TSP-1 activates TGF-b with increased Rho-kinase signalling, causing vasoconstriction. Flow cytometry revealed that a specific subset of IMs is the major source of pathologic TSP-1 in hypoxia. Intravenous depletion and parabiosis experiments demonstrated that these cells are circulating prior to recruitment into the interstitium. Rho-kinase-mediated vasoconstriction was a major downstream target of active TGF-b. Thbs1 deficient bone marrow (BM) protected against hypoxic-PH by blocking TGF-b activation and Rho-kinase-mediated vasoconstriction. In hypoxia-challenged mice, BM derived and circulating monocytes are recruited to become IMs which expressTSP-1, resulting in TGF-b activation and Rho-kinase-mediated vasoconstriction.

The project has been carried out in 2 parts.

Part 1: The data generated from year 1 has been published (Cardiovasc Res. 2020 Oct 1;116(12):2021-2030). The details and the raw data have been provided under the heading “Interstitial macrophage-derived thrombospondin-1 contributes to hypoxia-induced pulmonary hypertension”.

This data generated from this work has been published in the following journal:

Cardiovasc Res. 2020 Oct 1;116(12):2021-2030. doi: 10.1093/cvr/cvz304.

PMID: 31710666; PMCID: PMC7519884; DOI: 10.1093/cvr/cvz304

Part 2: while data generated from Year 2 and 3 are unpublished and are under revision on Journal of Clinical Investigation (JCI). The details and the raw data has been provided under the heading “The role of classical monocytes in hypoxic pulmonary hypertension.

Raw data: The raw data has been uploaded figure wise as part 2_Raw data file. The methods, experimental conditions, number of animal, and statistical analysis have been clearly explained in the respective figure legend. The flow cytometry raw data filed and excel sheet has been provided with description.

Please check README file for details.
RAQAMLI TRANSFORMATSIYA: KICHIK VA O'RTA BIZNESLAR UCHUN YANGI IMKONIYATLAR
Creators: name: Ro'zimov, Bahromjon
name: Xamidov, Muhammadjon
name: Amirov, Islomjon
Publisher: name: Zenodo
Year: 2025

description: Ushbu maqolada kichik va o‘rta bizneslar (KOB) uchun raqamli transformatsiyaning ahamiyati, uni joriy etishning asosiy yo‘nalishlari va zamonaviy texnologiyalar orqali ochilayotgan yangi imkoniyatlar tahlil qilinadi. Raqamli vositalar yordamida biznes jarayonlarini optimallashtirish, xaridorlar bilan interaktiv muloqot o‘rnatish, shuningdek, bozordagi raqobatbardoshlikni oshirish imkoniyatlari o‘rganiladi. Maqola, shuningdek, O‘zbekistonda KOB subyektlari raqamli transformatsiya yo‘lida duch kelayotgan muammolar va ularni bartaraf etish bo‘yicha tavsiyalarni ham o‘z ichiga oladi.
Supplementary Material for: Phototherapy with Narrow-Band UVB in Adult Guttate Psoriasis: Results and Patient Assessment
Creators: name: Fernández-Guarino, M.
name: Aboín-González, S.
name: Velázquez, D.
name: Barchino, L.
name: Cano, N.
name: Lázaro, P.
Publisher: name: Karger Publishers
Year: 2016
Subjects: Medicine

description: Background: Acute guttate psoriasis (AGP) is a distinctive clinical entity with good response to treatment with narrow-band ultraviolet B (NB-UVB). Objective: To investigate the results of NB-UVB phototherapy in adult patients with adult guttate psoriasis. Material and Methods: We carried out a prospective, open, and observational study. Patients over 18 years with more than 5% of body surface area affected were included. The PASI was assessed prior to and after treatment. The follow-up period was 18 months. After treatment, patients completed a simple questionnaire to assess their overall impression of the treatment. Results: The 67 adult patients with AGP included in this study had an initial PASI of 8.55 (SD 5.03). Patients were treated with a mean of 19.9 sessions (SD 13.5) and mean doses of 14 mJ/cm2 (SD 10.5). Of the 67 patients, 52 achieved PASI90 with 96.15% of PASI reduction, and of these, 46 (88%) maintained PASI90 during the 18 months of follow-up. Patients were very satisfied with the treatment. Discussion: AGP is a defined clinical entity with a variable course. Phototherapy with NB-UVB appears to be a very good option for treatment of AGP because of the good results obtained and patient satisfaction.
Accommodation-Free Head Mounted Display with Comfortable 3D Perception and an Enlarged Eye-box.
Creators: name: Shrestha, Pawan K
name: Pryn, Matt J
name: Jia, Jia
name: Chen, Jhen-Si
name: Fructuoso, Hector Navarro
name: Boev, Atanas
name: Zhang, Qing
name: Chu, Daping
Publisher: name: Apollo - University of Cambridge Repository
Year: 2019

description: An accommodation-free displays, also known as Maxwellian displays, keep the displayed image sharp regardless of the viewer's focal distance. However, they typically suffer from a small eye-box and limited effective field of view (FOV) which requires careful alignment before a viewer can see the image. This paper presents a high-quality accommodation-free head mounted display (aHMD) based on pixel beam scanning for direct image forming on retina. It has an enlarged eye-box and FOV for easy viewing by replicating the viewing points with an array of beam splitters. A prototype aHMD is built using this concept, which shows high definition, low colour aberration 3D augmented reality (AR) images with an FOV of 36°. The advantage of the proposed design over other head mounted display (HMD) architectures is that, due to the narrow, collimated pixel beams, the high image quality is unaffected by changes in eye accommodation, and the approach to enlarge the eye-box is scalable. Most importantly, such an aHMD can deliver realistic three-dimensional (3D) viewing perception with no vergence-accommodation conflict (VAC). It is found that viewing the accommodation-free 3D images with the aHMD presented in this work is comfortable for viewers and does not cause the nausea or eyestrain side effects commonly associated with conventional stereoscopic 3D or HMD displays, even for all day use.
Co-registration of optoacoustic tomography and magnetic resonance imaging data from murine tumour models.
Creators: name: Gehrung, Marcel
name: Tomaszewski, Michal
name: McIntyre, Dominick
name: Disselhorst, Jonathan
name: Bohndiek, Sarah
Publisher: name: Apollo - University of Cambridge Repository
Year: 2020
Subjects: image registration
Magnetic Resonance Imaging
Optoacoustic Tomography

description: As optoacoustic tomography (OT) emerges as a mainstream pre-clinical imaging modality, understanding the relationship between optoacoustic and other imaging biomarkers in the context of the underlying tissue biology becomes vitally important. Complementary insight into tumour vasculature and hypoxia can be gained using OT alongside magnetic resonance imaging (MRI)-based techniques. To evaluate the relationship between these metrics and the relative performance of the two modalities in assessment of tumour physiology, co-registration of their output imaging data is required. Unfortunately, this poses a significant challenge due to differences in animal positioning during imaging. Here, we present an integrated framework for registration of OT and MR image data in mice. Our framework combines a novel MR animal holder, to improve animal positioning during imaging, and a landmark-based software co-registration algorithm. We demonstrate that our protocol significantly improves registration of both body and tumour contours between these modalities, enabling more precise multi-modal tumour characterisation.
COL4A1-related autosomal recessive encephalopathy in 2 Turkish children.
Creators: name: Yaramis, Ahmet
name: Lochmüller, Hanns
name: Töpf, Ana
name: Sonmezler, Ece
name: Yilmaz, Elmasnur
name: Hiz, Semra
name: Yis, Uluc
name: Gungor, Serdal
name: Ipek Polat, Ayse
name: Edem, Pinar
name: Beltran, Sergi
name: Laurie, Steven
name: Yaramis, Aysenur
name: Horvath, Rita
name: Oktay, Yavuz
Publisher: name: Apollo - University of Cambridge Repository
Year: 2020

description: Objective:This study presents the neurologic phenotypes of 2 brothers with a novel homozygous COL4A1 mutation that was identified in a large Turkish consanguineous cohort of neurogenetic diseases. Methods:Whole-exome sequencing and bioinformatic analysis of consanguineous families with children affected by early-onset, neurogenetic disorders was performed using the RD-Connect Genome-Phenome Analysis Platform. We also performed clinical, EEG, and neuroimaging analyses in unaffected siblings and parents. Results:We have identified a homozygous missense mutation in COL4A1 (p.Gly1278Ser, NM_001845.5:c.3832G>T) in 2 siblings affected by small vessel brain disease with periventricular leukoencephalopathy and ocular defects. Presenting symptoms included mild weakness, hemiparetic gait, pyramidal findings, and seizures, whereas their intellectual and behavioral functions were normal. Both parents and 5 of the siblings (3 boys and 2 girls) were heterozygous for the variant. They did not show any clinical or laboratory signs of small vessel disease. Conclusions:COL4A1 has previously been associated with dominant small vessel disease of the brain and other organs, manifesting with high penetrance in heterozygous mutation carriers. Our findings provide evidence that COL4A1-related encephalopathy can be inherited in an autosomal recessive manner, which is important for counseling, prognosis, and treatment. Genotype-phenotype correlations remain to be established.
Ryanodine receptor modulation by caffeine challenge modifies Na+ current properties in intact murine skeletal muscle fibres.
Creators: name: Sarbjit-Singh, Sahib S
name: Matthews, Hugh R
name: Huang, Christopher L-H
Publisher: name: Apollo - University of Cambridge Repository
Year: 2020

description: We investigated effects of the ryanodine receptor (RyR) modulator caffeine on Na+ current (INa) activation and inactivation in intact loose-patch clamped murine skeletal muscle fibres subject to a double pulse procedure. INa activation was examined using 10-ms depolarising, V1, steps to varying voltages 0-80 mV positive to resting membrane potential. The dependence of the subsequent, INa inactivation on V1 was examined by superimposed, V2, steps to a fixed depolarising voltage. Current-voltage activation and inactivation curves indicated that adding 0.5 and 2 mM caffeine prior to establishing the patch seal respectively produced decreased (within 1 min) and increased (after ~2 min) peak INa followed by its recovery to pretreatment levels (after ~40 and ~30 min respectively). These changes accompanied negative shifts in the voltage dependence of INa inactivation (within 10 min) and subsequent superimposed positive activation and inactivation shifts, following 0.5 mM caffeine challenge. In contrast, 2 mM caffeine elicited delayed negative shifts in both activation and inactivation. These effects were abrogated if caffeine was added after establishing the patch seal or with RyR block by 10 μM dantrolene. These effects precisely paralleled previous reports of persistently (~10 min) increased cytosolic [Ca2+] with 0.5 mM, and an early peak rapidly succeeded by persistently reduced [Ca2+] likely reflecting gradual RyR inactivation with ≥1.0 mM caffeine. The latter findings suggested inhibitory effects of even resting cytosolic [Ca2+] on INa. They suggest potentially physiologically significant negative feedback regulation of RyR activity on Nav1.4 properties through increased or decreased local cytosolic [Ca2+], Ca2+-calmodulin and FKBP12.
Neurology-related protein biomarkers are associated with cognitive ability and brain volume in older age.
Creators: name: Harris, Sarah E
name: Cox, Simon R
name: Bell, Steven
name: Marioni, Riccardo E
name: Prins, Bram P
name: Pattie, Alison
name: Corley, Janie
name: Muñoz Maniega, Susana
name: Valdés Hernández, Maria
name: Morris, Zoe
name: John, Sally
name: Bronson, Paola G
name: Tucker-Drob, Elliot M
name: Starr, John M
name: Bastin, Mark E
name: Wardlaw, Joanna M
name: Butterworth, Adam S
name: Deary, Ian J
Publisher: name: Apollo - University of Cambridge Repository
Year: 2020

description: Identifying biological correlates of late life cognitive function is important if we are to ascertain biomarkers for, and develop treatments to help reduce, age-related cognitive decline. Here, we investigated the associations between plasma levels of 90 neurology-related proteins (Olink® Proteomics) and general fluid cognitive ability in the Lothian Birth Cohort 1936 (LBC1936, N = 798), Lothian Birth Cohort 1921 (LBC1921, N = 165), and the INTERVAL BioResource (N = 4451). In the LBC1936, 22 of the proteins were significantly associated with general fluid cognitive ability (β between -0.11 and -0.17). MRI-assessed total brain volume partially mediated the association between 10 of these proteins and general fluid cognitive ability. In an age-matched subsample of INTERVAL, effect sizes for the 22 proteins, although smaller, were all in the same direction as in LBC1936. Plasma levels of a number of neurology-related proteins are associated with general fluid cognitive ability in later life, mediated by brain volume in some cases.
PALB2 chromatin recruitment restores homologous recombination in BRCA1-deficient cells depleted of 53BP1.
Creators: name: Belotserkovskaya, Rimma
name: Raga Gil, Elisenda
name: Lawrence, Nicola
name: Butler, Richard
name: Clifford, Gillian
name: Wilson, Marcus D
name: Jackson, Stephen P
Publisher: name: Apollo - University of Cambridge Repository
Year: 2020

description: Funder: Wellcome Trust
description: Loss of functional BRCA1 protein leads to defects in DNA double-strand break (DSB) repair by homologous recombination (HR) and renders cells hypersensitive to poly (ADP-ribose) polymerase (PARP) inhibitors used to treat BRCA1/2-deficient cancers. However, upon chronic treatment of BRCA1-mutant cells with PARP inhibitors, resistant clones can arise via several mechanisms, including loss of 53BP1 or its downstream co-factors. Defects in the 53BP1 axis partially restore the ability of a BRCA1-deficient cell to form RAD51 filaments at resected DSBs in a PALB2- and BRCA2-dependent manner, and thereby repair DSBs by HR. Here we show that depleting 53BP1 in BRCA1-null cells restores PALB2 accrual at resected DSBs. Moreover, we demonstrate that PALB2 DSB recruitment in BRCA1/53BP1-deficient cells is mediated by an interaction between PALB2's chromatin associated motif (ChAM) and the nucleosome acidic patch region, which in 53BP1-expressing cells is bound by 53BP1's ubiquitin-directed recruitment (UDR) domain.
Chronic irradiation of human cells reduces histone levels and deregulates gene expression.
Creators: name: Lowe, Donna J
name: Herzog, Mareike
name: Mosler, Thorsten
name: Cohen, Howard
name: Felton, Sarah
name: Beli, Petra
name: Raj, Ken
name: Galanty, Yaron
name: Jackson, Stephen P
Publisher: name: Apollo - University of Cambridge Repository
Year: 2020

description: Over the past decades, there have been huge advances in understanding cellular responses to ionising radiation (IR) and DNA damage. These studies, however, were mostly executed with cell lines and mice using single or multiple acute doses of radiation. Hence, relatively little is known about how continuous exposure to low dose ionising radiation affects normal cells and organisms, even though our cells are constantly exposed to low levels of radiation. We addressed this issue by examining the consequences of exposing human primary cells to continuous ionising γ-radiation delivered at 6-20 mGy/h. Although these dose rates are estimated to inflict fewer than a single DNA double-strand break (DSB) per hour per cell, they still caused dose-dependent reductions in cell proliferation and increased cellular senescence. We concomitantly observed histone protein levels to reduce by up to 40%, which in contrast to previous observations, was not mainly due to protein degradation but instead correlated with reduced histone gene expression. Histone reductions were accompanied by enlarged nuclear size paralleled by an increase in global transcription, including that of pro-inflammatory genes. Thus, chronic irradiation, even at low dose-rates, can induce cell senescence and alter gene expression via a hitherto uncharacterised epigenetic route. These features of chronic radiation represent a new aspect of radiation biology.
Single-cell RNA-sequencing of differentiating iPS cells reveals dynamic genetic effects on gene expression.
Creators: name: Cuomo, Anna S E
name: Seaton, Daniel D
name: McCarthy, Davis J
name: Martinez, Iker
name: Bonder, Marc Jan
name: Garcia-Bernardo, Jose
name: Amatya, Shradha
name: Madrigal, Pedro
name: Isaacson, Abigail
name: Buettner, Florian
name: Knights, Andrew
name: Natarajan, Kedar Nath
name: Vallier, Ludovic
name: Marioni, John C
name: Chhatriwala, Mariya
name: Stegle, Oliver
Publisher: name: Apollo - University of Cambridge Repository
Year: 2020

description: Recent developments in stem cell biology have enabled the study of cell fate decisions in early human development that are impossible to study in vivo. However, understanding how development varies across individuals and, in particular, the influence of common genetic variants during this process has not been characterised. Here, we exploit human iPS cell lines from 125 donors, a pooled experimental design, and single-cell RNA-sequencing to study population variation of endoderm differentiation. We identify molecular markers that are predictive of differentiation efficiency of individual lines, and utilise heterogeneity in the genetic background across individuals to map hundreds of expression quantitative trait loci that influence expression dynamically during differentiation and across cellular contexts.
Security Compliance Assessment in DevOps - an Industry Report
Creators: name: Moyon, Fabiola
name: Mendez, Daniel
name: Angermeir
name: Gorschek, Tony
name: Voggenreiter, Markus
name: Bonvin, Pierre-Louis
Publisher: name: figshare
Year: 2024
Subjects: classificationCode: 461202
subject: Empirical software engineering
classificationCode: 460406
subject: Software and application security

description: Online Material that supports the research on Security Compliance Assessment in DevOps.We present excerpts of th RefA-AP and RefA-AR as well as instruments to conduct evaluation studies of RefA and assessment utilities.