title: Ð­ÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð³ÐµÐ½Ð¾Ð² Ð°ÐºÐ²Ð°Ð¿Ð¾ÑÐ¸Ð½Ð¾Ð² Ð»ÑÑÐµÑÐ½Ñ Ð¿ÑÐ¸ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ð¸ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ ÑÐ¸Ð¼Ð±Ð¸Ð¾Ð·Ð° Ñ Ð³ÑÐ¸Ð±Ð¾Ð¼ Ð°ÑÐ±ÑÑÐºÑÐ»ÑÑÐ½Ð¾Ð¹ Ð¼Ð¸ÐºÐ¾ÑÐ¸Ð·Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Expression of aquaporin genes in the development of effective symbiosis with arbuscular mycorrhiza fungus
Creators: name: Ð¤Ð¸Ð»Ð°ÑÐ¾Ð², ÐÐ°Ð²ÐµÐ»
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Ð°ÑÐ±ÑÑÐºÑÐ»ÑÑÐ½Ð°Ñ Ð¼Ð¸ÐºÐ¾ÑÐ¸Ð·Ð°
ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð³ÐµÐ½Ð¾Ð²
Ð°ÐºÐ²Ð°Ð¿Ð¾ÑÐ¸Ð½Ñ
rhizophagus irregularis
medicago lupulina
arbuscular mycorrhiza
gene expression
aquaporins

description: ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ:1) ÐÑÐ¾Ð²ÐµÑÑÐ¸ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½ÑÐ¹ Ð¾Ð±Ð·Ð¾Ñ Ð¿Ð¾ ÑÐµÐ¼Ðµ.2) ÐÑÐ¾Ð²ÐµÑÑÐ¸ Ð¿Ð¾ÑÐ°Ð´ÐºÑ, Ð²ÑÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ðµ Ð¸ ÑÐ±Ð¾Ñ ÑÐ°ÑÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ð° Ð´Ð»Ñ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾-Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹.3) ÐÑÐ²Ð¾Ð¸ÑÑ Ð¼ÐµÑÐ¾Ð´Ð¸ÐºÑ Ð¿Ð¾ÑÑÐ°Ð½Ð¾Ð²ÐºÐ¸ ÐÐ¦Ð  Ð² ÑÐµÐ°Ð»ÑÐ½Ð¾Ð¼ Ð²ÑÐµÐ¼ÐµÐ½Ð¸ (RT PCR).4) ÐÑÐ²Ð¾Ð¸ÑÑ Ð¼ÐµÑÐ¾Ð´Ð¸ÐºÑ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð´Ð°Ð½Ð½ÑÑ, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð² ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ RT PCR, Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ 2-âCT Ð¼ÐµÑÐ¾Ð´Ð°.5) Ð¡Ð´ÐµÐ»Ð°ÑÑ Ð²ÑÐ²Ð¾Ð´Ñ Ð¿Ð¾ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÐ¼ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ°Ð¼.ÐÐ±ÑÐµÐºÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â ÑÐ°ÑÑÐ¸ÑÐµÐ»ÑÐ½ÑÐ¹ Ð¸ Ð³ÑÐ¸Ð±Ð½Ð¾Ð¹ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»:1) Medicago lupulina. Ð ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐµ Ð±ÑÐ»Ð° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð° Ð»Ð¸Ð½Ð¸Ñ MlS-1 Ð¸Ð· ÑÐ¾ÑÑÐ¾Ð¿Ð¾Ð¿ÑÐ»ÑÑÐ¸Ð¸ ÐÐÐ32 Ð»ÑÑÐµÑÐ½Ñ ÑÐ¼ÐµÐ»ÐµÐ²Ð¸Ð´Ð½Ð¾Ð¹ (Medicago lupulina) [7].2) Plectranthus australis. ÐÐ Ð³ÑÐ¸Ð±Ñ ÑÐ²Ð»ÑÑÑÑÑ Ð¾Ð±Ð»Ð¸Ð³Ð°ÑÐ½ÑÐ¼Ð¸ ÑÐ¸Ð¼Ð±Ð¸Ð¾Ð½ÑÐ°Ð¼Ð¸, Ð¿Ð¾ÑÑÐ¾Ð¼Ñ Ð´Ð»Ñ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ñ ÐÐ Ð³ÑÐ¸Ð±Ð° (ÑÑÐ°Ð¼Ð¼ RCAM00320) ÑÑÐµÐ±ÑÐµÑÑÑ Ð½Ð°Ð»Ð¸ÑÐ¸Ðµ ÑÐ°ÑÑÐµÐ½Ð¸Ñ-ÑÐ¾Ð·ÑÐ¸Ð½Ð°. Ð¨ÑÐ°Ð¼Ð¼ Ð¿Ð¾Ð´Ð´ÐµÑÐ¶Ð¸Ð²Ð°ÐµÑÑÑ Ð½Ð° Ð½Ð°ÐºÐ¾Ð¿Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÐºÑÐ»ÑÑÑÑÐµ Ð¿Ð»ÐµÐºÑÑÐ°Ð½ÑÑÑÐ° ÑÐ¶Ð½Ð¾Ð³Ð¾ (Plectranthus australis).3) Ð ÐºÐ°ÑÐµÑÑÐ²Ðµ ÐÐ-Ð³ÑÐ¸Ð±Ð° Ð´Ð»Ñ Ð¸Ð½Ð¾ÐºÑÐ»ÑÑÐ¸Ð¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»ÑÑ ÑÑÑÐµÐºÑÐ¸Ð²Ð½ÑÐ¹ ÑÑÐ°Ð¼Ð¼ RCAM00320 Rhizophagus irregularis.Ð ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð¾ÑÐµÐ½ÐºÐ° ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Ð°ÐºÐ²Ð°Ð¿Ð¾ÑÐ¸Ð½Ð¾Ð² Ð½Ð° 7 ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ ÑÑÐ¾ÐºÐ°Ñ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ñ Ñ Ð´Ð²ÑÑ Ð²Ð°ÑÐ¸Ð°Ð½ÑÐ¾Ð² Ð¾Ð´Ð½Ð¾Ð¹ Ð»Ð¸Ð½Ð¸Ð¸ MlS-1 Ð¸Ð· ÑÐ¾ÑÑÐ¾Ð¿Ð¾Ð¿ÑÐ»ÑÑÐ¸Ð¸ ÐÐÐ32 M. lupulina. ÐÐ¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¾, ÑÑÐ¾ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð³ÐµÐ½Ð¾Ð² Ð½ÐµÐºÐ¾ÑÐ¾ÑÑÑ Ð°ÐºÐ²Ð°Ð¿Ð¾ÑÐ¸Ð½Ð¾Ð² M. lupulina Ð¿Ð¾Ð´ Ð²Ð»Ð¸ÑÐ½Ð¸ÐµÐ¼ ÐÐ-Ð³ÑÐ¸Ð±Ð¾Ð² Ð½Ð° ÑÐ°Ð·Ð½ÑÑ ÑÑÐ¾ÐºÐ°Ñ Ð¿ÑÐµÑÐµÑÐ¿ÐµÐ²Ð°ÐµÑ ÑÑÑÐµÑÑÐ²ÐµÐ½Ð½ÑÐµ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ. ÐÐ´Ð½Ð°ÐºÐ¾ ÑÐ¾ÑÐ½Ð¾ Ð²ÑÑÐ²Ð¸ÑÑ Ð¿Ð°ÑÑÐµÑÐ½Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð½Ð° Ð´Ð°Ð½Ð½Ð¾Ð¼ ÑÑÐ°Ð¿Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ Ð´Ð¾ÑÑÐ°ÑÐ¾ÑÐ½Ð¾ ÑÐ»Ð¾Ð¶Ð½Ð¾ Ð¸ ÑÑÐµÐ±ÑÑÑÑÑ Ð±Ð¾Ð»ÐµÐµ Ð´ÐµÑÐ°Ð»ÑÐ½ÑÐµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¿Ð¾ ÐºÐ°Ð¶Ð´Ð¾Ð¼Ñ Ð°ÐºÐ²Ð°Ð¿Ð¾ÑÐ¸Ð½Ñ Ð² Ð¾ÑÐ´ÐµÐ»ÑÐ½Ð¾ÑÑÐ¸. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ ÑÐ°Ð±Ð¾ÑÑ Ð²ÑÑÐ²Ð»ÐµÐ½Ñ Ð³ÐµÐ½Ñ Ð°ÐºÐ²Ð°Ð¿Ð¾ÑÐ¸Ð½Ð¾Ð², Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ ÐºÐ¾ÑÐ¾ÑÑÑ Ð²ÑÑÐ°Ð¶ÐµÐ½Ñ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ ÑÐ¸Ð»ÑÐ½Ð¾: NIP2.1, NIP4.2, PIP2.1, SIP1.3, TIP1.1 Ð¸ TIP4.1. ÐÐ»Ñ ÐºÐ°Ð¶Ð´Ð¾Ð³Ð¾ Ð¸Ð· Ð½Ð¸Ñ Ð²ÑÐ´Ð²Ð¸Ð½ÑÑÑ Ð¿ÑÐµÐ´Ð¿Ð¾Ð»Ð¾Ð¶ÐµÐ½Ð¸Ñ Ð¿Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð¼Ð¸ÐºÐ¾ÑÐ¸Ð·Ð°ÑÐ¸Ð¸ Ð½Ð° ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð¸ Ð² ÑÐ²ÑÐ·Ð¸ Ñ Ð¸Ñ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½ÑÐ¼Ð¸ ÑÑÐ½ÐºÑÐ¸ÑÐ¼Ð¸, Ð¾Ð¿Ð¸ÑÐ°Ð½Ð½ÑÐ¼Ð¸ Ð² Ð´ÑÑÐ³Ð¸Ñ ÑÐ°Ð±Ð¾ÑÐ°Ñ Ð´Ð»Ñ Ð³ÐµÐ½Ð¾Ð²-Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¾Ð² Ð½Ð° Medicago truncatula.
description: The tasks of the study:1) Conduct a literary review on the topic;2) Plan, grow and collect plant material for molecular genetic research;3) Master the technique of real-time PCR (RT PCR);4) Master the methodology of analyzing obtained data which is a result of RT PCR using the 2 -âCT method;5) Draw conclusions based on the results obtained.Objects of research â plant and fungi material:1) Medicago lupulina. In the experiment, the MlS-1 line was used from the varietal population of VIC32 alfalfa hop (Medicago lupulina) [7].2) Plectranthus australis. AM fungi are obligate symbionts, therefore, the presence of a host plant is required for the development of an AM fungus (strain RCAM00320). The strain is maintained on the accumulative culture of the southern plectranthus (Plectranthus australis).3) An effective strain RCAM00320 Rhizophagus irregularis was used as an AM fungus for inoculation.During the research, the expression of aquaporin genes was evaluated at 7 different developmental periods in two different M. lupulina lines. It was determined that the expression of genes of M. lupulina aquaporins under the influence of AM fungi undergoes significant changes at different periods. However, it was quite difficult to accurately identify expression patterns at this stage of research and more detailed studies on each aquaporin separately were required. As a result of the work, the aquaporin genes whose expression was changing were identified: NIP2.1, NIP4.2, PIP2.1, SIP1.3, TIP1.1 and TIP4.1. For each of them, assumptions have been made on the effect of mycorrhization on expression and in connection with their possible functions described in other works for orthologous genes on Medicago truncatula.