title: Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° ÑÐµÑ Ð½Ð¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ð¾Ð² Ð¸Ð· Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹ Chlorella
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Development of technologies for producing carotenoids from biomass of Chlorella microalgae
Creators: name: ÐÐ¸ÐºÐ¸ÑÐ¸Ð½Ð°, ÐÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ñ
Chlorella sorokiniana
Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð½Ð¾Ðµ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
Ð»Ð¸Ð¾ÑÐ¸Ð»ÑÐ½Ð°Ñ ÑÑÑÐºÐ°
ÑÐºÑÑÑÐ°ÐºÑÐ¸Ñ Ð¿Ð¸Ð³Ð¼ÐµÐ½ÑÐ¾Ð²
carotinoids
directed cultivation
freeze drying
pigment extraction

description: ÐÐ° 82 Ñ., 46 ÑÐ¸ÑÑÐ½ÐºÐ¾Ð², 14 ÑÐ°Ð±Ð»Ð¸Ñ, 4 Ð¿ÑÐ¸Ð»Ð¾Ð¶ÐµÐ½Ð¸Ñ. ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ ÑÑÐ»Ð¾Ð²Ð¸Ð¹, Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÑÑÐ¸Ñ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð½Ð° ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð³ÐµÐ½ÐµÐ· Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹ Chlorella Ð¿ÑÐ¸ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ Ð² Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ½Ð¾Ð¼ Ð±Ð¸Ð¾ÑÐµÐ°ÐºÑÐ¾ÑÐµ, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¿Ð¾Ð´Ð±Ð¾ÑÑ ÑÑÐ»Ð¾Ð²Ð¸Ð¹ Ð¿Ð¾Ð´Ð³Ð¾ÑÐ¾Ð²ÐºÐ¸ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð¸ Ð¸Ð·Ð²Ð»ÐµÑÐµÐ½Ð¸Ñ Ð¸Ð· Ð½ÐµÑ ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ð¾Ð². ÐÐ°Ð´Ð°ÑÐ¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: - Ð¸Ð·ÑÑÐ¸ÑÑ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½ÑÐµ Ð¸ÑÑÐ¾ÑÐ½Ð¸ÐºÐ¸ Ð¿Ð¾ ÑÐµÐ¼Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ; - Ð¿Ð¾Ð´Ð¾Ð±ÑÐ°ÑÑ ÑÑÐ»Ð¾Ð²Ð¸Ñ Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð½Ð¾Ð³Ð¾ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¡hlorella sorokiniana Ð´Ð»Ñ Ð¼Ð°ÐºÑÐ¸Ð¼Ð°Ð»ÑÐ½Ð¾Ð¹ Ð½Ð°ÑÐ°Ð±Ð¾ÑÐºÐ¸ ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ð¾Ð²; - Ð²ÑÐ±ÑÐ°ÑÑ Ð¾Ð¿ÑÐ¸Ð¼Ð°Ð»ÑÐ½ÑÐ¹ Ð´Ð»Ñ Ð²ÑÑÐ¾Ð´Ð° Ð¿Ð¸Ð³Ð¼ÐµÐ½ÑÐ¾Ð² ÑÐ¿Ð¾ÑÐ¾Ð± ÑÑÑÐºÐ¸ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ð¸; - Ð¿Ð¾Ð´Ð¾Ð±ÑÐ°ÑÑ ÑÐµÐ¶Ð¸Ð¼ ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸ ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ð¾Ð² Ñ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐ¸Ð¼ Ð¾Ð¼ÑÐ»ÐµÐ½Ð¸ÐµÐ¼ ÑÐ»Ð¾ÑÐ¾ÑÐ¸Ð»Ð»Ð°; - Ð¿Ð¾Ð»ÑÑÐ¸ÑÑ Ð¼Ð°ÑÐ»ÑÐ½ÑÐ¹ ÑÐºÑÑÑÐ°ÐºÑ ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ð¾Ð² Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð¿ÐµÑÐµÑÐ°ÑÑÐ²Ð¾ÑÐµÐ½Ð¸Ñ. Ð Ð°Ð±Ð¾ÑÐ° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ ÐÐ¨ÐÐ¸ÐÐ Ð¡ÐÐ±ÐÐ£. ÐÑÐ»Ð¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ñ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÑ Ð¿Ð¾ Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð½Ð¾Ð¼Ñ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹ (Ð¿ÑÐ¸ ÑÑÐ¾Ð¼ ÑÐ¾ÑÑÐ¾ÑÐ½Ð¸Ðµ Ð¿Ð¾Ð¿ÑÐ»ÑÑÐ¸Ð¸ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÑÐ»Ð¸ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸ÑÐµÑÐºÐ¸), Ð²ÑÑÑÑÐ¸Ð²Ð°Ð½Ð¸Ñ Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ, Ð¿Ð¾Ð´Ð±Ð¾ÑÑ ÑÑÐ»Ð¾Ð²Ð¸Ð¹ ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸ Ð¸ ÑÐ°Ð·Ð´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¿Ð¸Ð³Ð¼ÐµÐ½ÑÐ¾Ð² Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ Ð¾Ð¼ÑÐ»ÐµÐ½Ð¸Ñ ÑÐ»Ð¾ÑÐ¾ÑÐ¸Ð»Ð»Ð°. ÐÑÑÐ¾Ð´ Ð¿Ð¸Ð³Ð¼ÐµÐ½ÑÐ¾Ð² Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÑÐ»Ð¸ ÑÐ¿ÐµÐºÑÑÐ¾ÑÐ¾ÑÐ¾Ð¼ÐµÑÑÐ¸ÑÐµÑÐºÐ¸. ÐÐµÑÐµÑÐ°ÑÑÐ²Ð¾ÑÐµÐ½Ð¸Ðµ ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ð¾Ð² Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»Ð¸ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ ÑÐ¾ÑÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð³Ð¾ Ð¸ÑÐ¿Ð°ÑÐ¸ÑÐµÐ»Ñ. Ð ÑÐ¾Ð´Ðµ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ» Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ½ Ð¸ Ð¸Ð·ÑÑÐµÐ½ Ð¿ÑÐ¾ÑÐµÑÑ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ð¸ Ð¡hlorella Ð¿ÑÐ¸ Ð²Ð¾Ð·Ð´ÐµÐ¹ÑÑÐ²Ð¸Ð¸ ÑÐ»ÑÑÑÐ°Ð·Ð²ÑÐºÐ° Ð¸ ÑÐ»ÑÑÑÐ°ÑÐ¸Ð¾Ð»ÐµÑÐ°, ÐºÐ°Ðº ÑÑÑÐµÑÑÐ¾Ð²ÑÑ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð², ÑÐ¿Ð¾ÑÐ¾Ð±Ð½ÑÑ Ð²Ð»Ð¸ÑÑÑ Ð½Ð° ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð³ÐµÐ½ÐµÐ·. Ð Ð°ÑÑÐ¼Ð¾ÑÑÐµÐ½Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ ÑÐ¿Ð¾ÑÐ¾Ð±Ð¾Ð² ÑÑÑÐºÐ¸ Ð½Ð° Ð²ÑÑÐ¾Ð´ ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ð¾Ð² Ð±Ð¸Ð¾Ð¼Ð°ÑÑÑ Ð¡hlorella, Ð¿Ð¾Ð´Ð¾Ð±ÑÐ°Ð½ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ Ð±Ð»Ð°Ð³Ð¾Ð¿ÑÐ¸ÑÑÐ½ÑÐ¹ Ð´Ð»Ñ Ð¸Ð·Ð²Ð»ÐµÑÐµÐ½Ð¸Ñ Ð¼Ð°ÐºÑÐ¸Ð¼Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð° ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ð¾Ð² ÑÐµÐ¶Ð¸Ð¼ ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐ¹ Ð¿Ð¾ Ð¸ÑÐ¾Ð³Ñ ÑÐ°Ð±Ð¾ÑÑ Ð¼Ð°ÑÐ»ÑÐ½ÑÐ¹ ÑÐºÑÑÑÐ°ÐºÑ ÐºÐ°ÑÐ¾ÑÐ¸Ð½Ð¾Ð¸Ð´Ð¾Ð² Ð¼Ð¾Ð¶Ð½Ð¾ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°ÑÑ Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð´Ð¾Ð±Ð°Ð²ÐºÐ¸ Ðº Ð¿ÑÐ¾Ð´ÑÐºÑÐ°Ð¼ Ð¿Ð¸ÑÐ°Ð½Ð¸Ñ Ð¸Ð»Ð¸ ÐºÐ°Ðº Ð¾ÑÐ½Ð¾Ð²Ñ Ð´Ð»Ñ ÑÐ¾Ð·Ð´Ð°Ð½Ð¸Ñ Ð»ÐµÑÐµÐ±Ð½Ð¾-Ð¿ÑÐ¾ÑÐ¸Ð»Ð°ÐºÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ°.
description: 82 pages, 46 figures, 14 tables, 4 appendices. This work is devoted to the study of conditions that affect on the carotenogenesis of microalgae Chlorella cultivated in a laboratory bioreactor, and the selection of conditions for the preparation of biomass and extraction of carotenoids from it. Research objectives: - study literary sources on the topic of research; - choose conditions for directed cultivation of Chlorella sorokiniana for maximum production of carotenoids; - choose the optimal method for drying microalgae biomass for pigment yield; - choose a mode of extraction of carotenoids with subsequent saponification of chlorophyll; - get the carotenoid oil extract by re-dissolving. The work was fulfilled on the premises of VShBiPP SPbPU. Experiments on directed cultivation of microalgae were carried out (the state of the population was determined microscopically), drying of biomass, selection of extraction conditions and separation of pigments by the method of saponification of chlorophyll. The yield of pigments was determined spectrophotometrically. Re-dissolution of carotenoids was carried out using a rotary evaporator. In the course of the work, the process of cultivating Chlorella microalgae under the influence of ultrasound and ultraviolet radiation, as stress factors capable of affecting carotenogenesis, was carried out and studied. The effect of drying methods on the yield of carotenoids of Chlorella biomass is considered, the extraction mode most favorable for extracting the maximum amount of carotenoids is selected. The oil extract of carotenoids can be used as an additive to food products or as a basis for creating a therapeutic and prophylactic drug.