title: Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÑÐµÑÐºÐ¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ñ Ð¸ Ð¼Ð¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÐºÐ¸Ð½ÐµÑÐ¸ÐºÐ¸ Ð¿ÑÐ¾ÑÐµÑÑÐ° Ð¿ÑÐ¸ Ð°Ð»Ð»ÐµÐ»ÑÐ½Ð¾Ð¹ Ð´Ð¸ÑÐºÑÐ¸Ð¼Ð¸Ð½Ð°ÑÐ¸Ð¸ Ð¾Ð´Ð½Ð¾Ð½ÑÐºÐ»ÐµÐ¾ÑÐ¸Ð´Ð½Ð¾Ð³Ð¾ Ð¿Ð¾Ð»Ð¸Ð¼Ð¾ÑÑÐ¸Ð·Ð¼Ð°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Design of a diagnostic system and kinetic modeling of process in allelic discrimination of single-nucleotide polymorphism
Creators: name: ÐÑÐ¾Ð²Ð¸Ð½, ÐÐ½Ð´ÑÐµÐ¹
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: ÐÐ¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð°Ñ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ñ
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description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐµ Ð¸ Ð¾Ð¿ÑÐ¸Ð¼Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð²ÑÑÐ²Ð»ÐµÐ½Ð¸Ñ Ð¾Ð´Ð½Ð¾Ð½ÑÐºÐ»ÐµÐ¾ÑÐ¸Ð´Ð½ÑÐ¹ Ð¿Ð¾Ð»Ð¸Ð¼Ð¾ÑÑÐ¸Ð·Ð¼Ð¾Ð² Ð´Ð»Ñ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð½Ð°ÑÐ»ÐµÐ´ÑÑÐ²ÐµÐ½Ð½ÑÑ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ð¹. ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð¸Ñ: 1. ÐÐ·ÑÑÐµÐ½Ð¸Ðµ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð° ÐºÐ¾Ð½ÐºÑÑÐ¸ÑÑÑÑÐ¸Ñ ÑÐµÐ°ÐºÑÐ¸Ð¹ 2. ÐÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð° ÐºÐ¾Ð½ÐºÑÑÐ¸ÑÑÑÑÐ¸Ñ ÑÐµÐ°ÐºÑÐ¸Ð¹ Ð´Ð»Ñ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ¸ Ð²ÑÑÐ¾ÐºÐ¾ÑÑÑÐµÐºÑÐ¸Ð²Ð½ÑÑ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÐ¸ÑÑÐµÐ¼ 3. Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð¸ Ð°Ð¿ÑÐ¾Ð±Ð°ÑÐ¸Ñ Ð°Ð½Ð°Ð»Ð¸ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð¼Ð¾Ð´ÐµÐ»Ð¸, Ð¾Ð¿Ð¸ÑÑÐ²Ð°ÑÑÐµÐ¹ ÐºÐ¸Ð½ÐµÑÐ¸ÐºÑ ÐÐ¦Ð  Ð² ÑÐµÐ¶Ð¸Ð¼Ðµ ÑÐµÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð²ÑÐµÐ¼ÐµÐ½Ð¸ Ð Ð°Ð±Ð¾ÑÐ° Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´Ð¸Ð»Ð°ÑÑ Ð½Ð° Ð±Ð°Ð·Ðµ ÐÐÐ Â«ÐÐ¸Ð³Ð»ÑÂ», Ð³Ð´Ðµ ÑÐ¾Ð±Ð¸ÑÐ°Ð»Ð°ÑÑ Ð·Ð½Ð°ÑÐ¸ÑÐµÐ»ÑÐ½Ð°Ñ ÑÐ°ÑÑÑ ÑÐ°ÐºÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ð°: ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ ÐÐ¦Ð -Ð Ð Ð´Ð»Ñ Ð³ÐµÐ½Ð¾ÑÐ¸Ð¿Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¾Ð´Ð½Ð¾Ð½ÑÐºÐ»ÐµÐ¾ÑÐ¸Ð´Ð½Ð¾Ð³Ð¾ Ð¿Ð¾Ð»Ð¸Ð¼Ð¾ÑÑÐ¸Ð·Ð¼Ð°. ÐÑÐ»Ð¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ð¸ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð° ÐºÐ¾Ð½ÐºÑÑÐ¸ÑÑÑÑÐµÐ¹ ÑÐµÐ°ÐºÑÐ¸Ð¸ Ð½Ð° ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð¸ ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐ½Ð¾ÑÑÑ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÐ¸ÑÑÐµÐ¼. ÐÐ±ÑÐ°Ð±Ð¾ÑÐºÐ° ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¸ Ð°Ð½Ð°Ð»Ð¸ÑÐ¸ÑÐµÑÐºÐ¾Ðµ Ð¼Ð¾Ð´ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´Ð¸Ð»Ð¸ÑÑ Ð² ÑÑÐµÐ´Ðµ Matlab. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð° Ð²ÑÑÐ¾ÐºÐ¾ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð°Ñ Ð¸ ÑÐ¿ÐµÑÐ¸ÑÐ¸ÑÐ½Ð°Ñ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÑÐµÑÐºÐ°Ñ ÑÐ¸ÑÑÐµÐ¼Ð° Ð³ÐµÐ½Ð¾ÑÐ¸Ð¿Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð³Ð°Ð½Ð³Ð»Ð¸Ð¾Ð·Ð¸Ð´Ð¾Ð·Ð° Ð¿ÐµÑÐ²Ð¾Ð³Ð¾ ÑÐ¸Ð¿Ð° Ð¸ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð° Ð¼ÐµÑÐ¾Ð´Ð¸ÐºÐ° Ð´Ð»Ñ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ ÑÐ¾Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ñ Ð¼ÑÑÐ°Ð½ÑÐ½Ð¾Ð¹ Ð¸ Ð´Ð¸ÐºÐ¾ÑÐ¸Ð¿Ð½Ð¾Ð¹ Ð°Ð»Ð»ÐµÐ»Ð¸ Ð³ÐµÐ½Ð° GLB-1 Ñ Ð³ÐµÑÐµÑÐ¾Ð·Ð¸Ð³Ð¾ÑÐ½Ð¾Ð³Ð¾ Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ð° Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÐÐ¦Ð -Ð Ð.
description: The given work is devoted to design and improvement methods of single-nucleotide discrimination for diagnostics of hereditary diseases. The research set the following goals: 1. Studying the mechanism of competing reactions 2. Application of competing reactions to development of high-performance diagnostic systems. 3. Development and testing of analytical model describing the real-time PCR kinetics with competitive reactions. The work was fulfilled on the premises of LLC Â«BeagleÂ», which included collection of factual materials, i.e. PCR results of single-nucleotide polymorphism genotyping. The calculations, made during the research, show how competing reactions influences on efficiency and specificity of diagnostic systems. The analysis was conducted by the method of mathematical modeling and experimental data processing with the use of Matlab. In the result of studying there was produced a highly effective and specific diagnostic system for genotyping gangliosidosis of the first type and was developed a method for determining the ratio of the mutant and wildtype allele of the GLB-1 gene in a heterozygous organism using real-time PCR.