title: Ð­ÐºÑÑÑÐ°ÐºÑÐ¸Ñ ÑÑÐ³Ð¾ÑÑÐµÑÐ¸Ð½Ð° Ð¸Ð· Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹ Saccharomyces cerevisiae Ð´Ð»Ñ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ Ð²Ð¸ÑÐ°Ð¼Ð¸Ð½Ð° D
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Extraction of ergosterol from Saccharomyces cerevisiae yeast for production of vitamin D
Creators: name: ÐÐ¾ÑÐ½Ð¸Ð»Ð¾Ð², ÐÐ°ÑÐ¸Ð¹
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: Ð²Ð¸ÑÐ°Ð¼Ð¸Ð½ D
ÑÑÐ³Ð¾ÑÑÐµÑÐ¸Ð½
Ð´ÑÐ¾Ð¶Ð¶Ð¸
ÑÐºÑÑÑÐ°ÐºÑÐ¸Ñ
Ð¿ÑÐ¾Ð²Ð¸ÑÐ°Ð¼Ð¸Ð½
yeasts
ergosterol
vitamin D
extraction

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¾Ð¿Ð¸ÑÐ°Ð½Ð¸Ñ Ð¸ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð¼ÐµÑÐ¾Ð´Ð° ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸ ÑÑÐ³Ð¾-ÑÑÐµÑÐ¸Ð½Ð° Ð¸Ð· Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹ Ð¿ÑÑÐµÐ¼ Ð¿ÐµÑÐºÐ¾Ð»ÑÑÐ¸Ð¸ Ñ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐ¸Ð¼ ÐµÐ³Ð¾ Ð¿ÑÐµÐ²ÑÐ°ÑÐµÐ½Ð¸ÐµÐ¼ Ð² Ð²Ð¸ÑÐ°Ð¼Ð¸Ð½ D2. ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: 1. ÐÐ¾Ð´Ð±Ð¾Ñ Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹ Ñ Ð¼Ð°ÐºÑÐ¸Ð¼Ð°Ð»ÑÐ½ÑÐ¼ ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸ÐµÐ¼ ÑÑÐ³Ð¾ÑÑÐµÑÐ¸Ð½Ð°; 2. Ð Ð°Ð·ÑÑÑÐµÐ½Ð¸Ðµ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ ÑÑÐµÐ½ÐºÐ¸ Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹ ÑÐ¸Ð·Ð¸ÑÐµÑÐºÐ¸Ð¼Ð¸ Ð¼ÐµÑÐ¾Ð´Ð°Ð¼Ð¸; 3. Ð Ð°ÑÑÐµÑ Ð¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ðµ ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸ ÑÑÐ³Ð¾ÑÑÐµÑÐ¸Ð½Ð°; 4. ÐÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ðµ Ð¿ÑÐµÐ²ÑÐ°ÑÐµÐ½Ð¸Ñ ÑÑÐ³Ð¾ÑÑÐµÑÐ¸Ð½Ð° Ð² Ð²Ð¸ÑÐ°Ð¼Ð¸Ð½ D2; 5. ÐÐ°ÑÐµÑÑÐ²ÐµÐ½Ð½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð· ÐºÐ¾Ð½ÐµÑÐ½Ð¾Ð³Ð¾ Ð¿ÑÐ¾Ð´ÑÐºÑÐ°-Ð²Ð¸ÑÐ°Ð¼Ð¸Ð½Ð° D2. ÐÐ±ÑÐµÐºÑÐ°Ð¼Ð¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð² Ð´Ð°Ð½Ð½Ð¾Ð¹ Ð´Ð¸Ð¿Ð»Ð¾Ð¼Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ Ð²ÑÑÑÑÐ¿Ð°Ð»Ð¸ â Ð´ÑÐ¾Ð¶Ð¶Ð¸ Ð¿ÑÐµÑÑÐ¾Ð²Ð°Ð½Ð½ÑÐµ ÑÐ¾Ð´Ð° Saccharomyces, Ð¸Ð·Ð¾Ð¿ÑÐ¾Ð¿Ð°Ð½Ð¾Ð», Ð³Ð¸Ð´ÑÐ¾ÐºÑÐ¸Ð´ Ð½Ð°ÑÑÐ¸Ñ. Ð Ð°Ð±Ð¾ÑÐ° Ð±ÑÐ»Ð° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ ÐÐ¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑÐ°: Ð±Ð¾Ð»ÑÑÐ°Ñ ÑÐ°ÑÑÑ Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð¼ÑÑ Ð¼Ð°Ð½Ð¸Ð¿ÑÐ»ÑÑÐ¸Ð¹ Ð±ÑÐ»Ð¾ ÑÐ´ÐµÐ»Ð°Ð½Ð¾ Ð² Ð±Ð¸Ð¾ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ ÐÐÐ¡Ð¸Ð. ÐÑÐ¸ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð¸Ð¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ ÑÐºÑÑÑÐ°ÐºÑÐ¸Ñ ÑÑÐ³Ð¾ÑÑÐµÑÐ¸Ð½Ð° Ð±ÑÐ»Ð° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð¸Ð· Ð´ÑÐ¾Ð¶Ð¶ÐµÐ²Ð¾Ð¹ Ð¼Ð°ÑÑÑ. Ð ÐºÐ°ÑÐµÑÑÐ²Ðµ ÑÐºÑÑÑÐ°Ð³ÐµÐ½ÑÐ° Ð±ÑÐ» Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½ Ð¸Ð·Ð¾Ð¿ÑÐ¾Ð¿Ð¸Ð»Ð¾Ð²ÑÐ¹ ÑÐ¿Ð¸ÑÑ. ÐÑÐ»Ð¾ Ð¿ÑÐ¾Ð¸Ð·Ð²ÐµÐ´ÐµÐ½Ð¾ Ð¸Ð·Ð²Ð»ÐµÑÐµÐ½Ð¸Ðµ ÑÑÐ³Ð¾ÑÑÐµÑÐ¸Ð½Ð°. ÐÐ°Ð»ÐµÐµ, Ð¿ÑÐ¾Ð¸Ð·Ð²ÐµÐ´ÐµÐ½Ð¾ ÐµÐ³Ð¾ Ð¿ÑÐµ-Ð²ÑÐ°ÑÐµÐ½Ð¸Ðµ Ð² Ð²Ð¸ÑÐ°Ð¼Ð¸Ð½ D2 Ñ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐ¸Ð¼ ÐµÐ³Ð¾ ÐºÐ°ÑÐµÑÑÐ²ÐµÐ½Ð½ÑÐ¼ Ð¸ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²ÐµÐ½Ð½ÑÐ¼ Ð°Ð½Ð°Ð»Ð¸Ð·Ð¾Ð¼. Ð ÑÐ¾Ð´Ðµ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¾ Ð½Ð°Ð¹Ð´ÐµÐ½Ð¾, ÑÑÐ¾ ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ðµ ÑÐ³Ð¾ÑÑÐµÑÐ¸Ð½Ð° Ð² Ð´ÑÐ¾Ð¶Ð¶Ð°Ñ Ð·Ð°Ð²Ð¸ÑÐ¸Ñ Ð¾Ñ Ð¸Ñ Ð¶Ð¸Ð·Ð½ÐµÐ½Ð½Ð¾Ð¹ ÑÐ°Ð·Ñ. Ð¢Ð°ÐºÐ¶Ðµ Ð±ÑÐ»Ð¾ ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¾, ÑÑÐ¾ Ð¸Ð·Ð¾Ð¿ÑÐ¾Ð¿Ð¸Ð»Ð¾Ð²ÑÐ¹ ÑÐ¿Ð¸ÑÑ Ð¾Ð±Ð»Ð°Ð´Ð°ÐµÑ Ð²ÑÑÐ¾ÐºÐ¾Ð¹ Ð¸Ð·Ð±Ð¸ÑÐ°ÑÐµÐ»ÑÐ½Ð¾ÑÑÑÑ Ð² Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ð¸ ÑÑÐµÑÐ¾Ð¸Ð´Ð¾Ð¿Ð¾Ð´Ð¾Ð±Ð½ÑÑ Ð²ÐµÑÐµÑÑÐ².
description: This work is devoted to the description and application of the method of extrac-tion of ergosterol from yeast by percolation with its subsequent conversion into vita-min D2. The tasks that were solved during the study: 1. Selection of yeasts contents the maximum ergosterol substance; 2. Physical destruction of yeast cell walls; 3. Calculation and carrying out of ergosterol extraction; 4. Carrying out the conversion of ergosterol to vitamin D2; 5. Qualitative analysis of vitamin D2. During the research, the extraction of ergosterol was carried out from the yeast mass. Isopropyl alcohol was used as an extractant. Ergosterol was extracted. Further, it was converted into vitamin D2 with its subsequent qualitative analysis. In the course of study, it was found that the content of ergosterol in yeast depends on their life phase. It was also found that isopropyl alcohol has a high selectivity against steroid-like substances.