Additional file 1 of Lung perfusion during veno-venous extracorporeal membrane oxygenation in a model of hypoxemic respiratory failure
Creators: name: Mendes, Pedro Vitale
name: Park, Marcelo
name: de Azevedo, Luciano Cesar Pontes
name: Morais, Caio Cesar Araujo
name: Amato, Marcelo Brito Passos
name: Costa, Eduardo Leite Vieira
Publisher: name: figshare
Year: 2022
Subjects: subject: Biophysics
subject: Medicine
subject: Cell Biology
subject: Molecular Biology
subject: Physiology
subject: FOS: Biological sciences
subject: Evolutionary Biology
subject: 69999 Biological Sciences not elsewhere classified
subject: Cancer
subject: Computational Biology

description: Additional file 1: Figure S1. Schematic drawing representing experimental model without ECMO canulation. Deoxygenated blood return from peripheral compartment to the right side of the heart. The heart pumps the blood to the native lung in which gas exchange occurs. The oxygenated blood return to the left side of the heart and is posteriorly pumped to the peripheral compartment. Figure S2. Schematic drawing representing experimental model after ECMO canulation but without gas flow (Gas Flow OFF). Deoxygenated blood returning from peripheral compartment pass partially through ECMO system. However, since there is no gas flow in the circuit, the blood still returns to the circulation deoxygenated and gas exchange still occurs exclusively on native lungs. Figure S3. Schematic drawing representing experimental model after ECMO canulation and with gas flow (Gas Flow ON). Deoxygenated blood returning from peripheral compartment pass partially through ECMO system. Gas exchange occurs in the ECMO system and blood returns oxygenated to the right side of the heart. The heart pumps the blood to the native lung in which gas exchange also occurs (acting as two oxygenators in series). The oxygenated blood return to the left side of the heart and is posteriorly pumped to the peripheral compartment. Figure S4. (A) : Lung ventilation before and after selective intubation with left lung atelectasis without ECMO circuit. Left lung ventilation was reduced to near zero values and returned to normal values after returning bilateral ventilation. (B): Left lung perfusion before and after unilateral ventilation with left lung atelectasis without ECMO circuit. Left lung perfusion significantly decreased after selective intubation and returned to previous values after returning to bilateral ventilation. (C): Pulmonary shunt before and after unilateral ventilation with left lung atelectasis without ECMO circuit. Pulmonary shunt significantly increased after selective intubation and returned to previous values after returning to bilateral ventilation. (E): PAPm = Mean Pulmonary Artery Pressure. PAPm before and after unilateral ventilation with left lung atelectasis without ECMO circuit. PAPm significantly increased after selective intubation and returned to previous values after returning to bilateral ventilation. Data was not homogenous to all animals. Table S1. Comparison between stages Baseline, Stage 1 and Stage 2. Before installation of ECMO circuit in two different scenarios: Bilateral ventilation and unilateral ventilation. PAC SvO2 denotes venous oxygen saturation acquired in the Pulmonary Arterial Catheter; PaO2 and PvO2 denotes arterial and venous oxygen partial pressure, respectively; PaCO2 denotes arterial carbon dioxide partial pressure; SaO2 denotes arterial oxygen saturation and BE denotes Base Excess. Table S2. Comparison between stages 3 (S3) and 4 (S4). Variables without (Gas Flow OFF) and with ECMO support (Gas Flow ON). PAC SvO2 denotes venous oxygen saturation acquired in the Pulmonary Arterial Catheter; PaO2 and PvO2 denotes arterial and venous oxygen partial pressure, respectively; PaCO2 denotes arterial carbon dioxide partial pressure; SaO2 denotes arterial oxygen saturation and BE denotes Base Excess. Figure S5. (A):PvO2=Venous Oxygen partial pressure. PvO2 significantly increased after initiation of ECMO support. (B): PAPm=Mean Pulmonary Artery Pressure. PAPm did not change after initiation of ECMO support during bilateral ventilation and no previous hypoxemia. (C): Left/Right Lung perfusion before and after initiation of ECMO support during bilateral ventilation. ECMO support by itself did not promote any variation on lung perfusion. (D): Anterior/Posterior lung perfusion before and after initiation of ECMO support during bilateral ventilation. ECMO support by itself did not promote any variation on lung perfusion. Table S3. Comparison between stages 3 (S3) and 5 (S5). Bilateral ventilation (S3) and Unilateral Ventilation (S5) after blood circulation through ECMO circuit. PAC SvO2 denotes venous oxygen saturation acquired in the Pulmonary Arterial Catheter; PaO2 and PvO2 denotes arterial and venous oxygen partial pressure, respectively; PaCO2 denotes arterial carbon dioxide partial pressure; SaO2 denotes arterial oxygen saturation and BE denotes Base Excess. Figure S6. (A): Left lung ventilation before and after selective intubation and left lung collapse with blood flow through ECMO circuit. There was a significant reduction in left lung ventilation. (B): PvO2=Venous Oxygen partial pressure. PvO2 remained stable after selective lung ventilation and left lung collapse with blood flow through ECMO circuit. (C): Left lung perfusion before and after selective intubation and left lung collapse with blood flow through ECMO circuit. Blood flow through ECMO did not affect the capacity of the EIT to detect the reduction in left lung perfusion.