title: ÐÐ¾Ð¸ÑÐº Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½ÑÑ Ð¿ÐµÑÐµÐºÐ»ÑÑÐ°ÑÐµÐ»ÐµÐ¹ Ð³Ð»ÑÑÐ°Ð¼Ð°ÑÐ½ÑÑ Ð¸ Ð°Ð½Ð°ÑÑÐ¾Ð¿Ð½ÑÑ ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð² AMPA Ð¸ NMDA ÑÐ¸Ð¿Ð¾Ð² ÑÑÐµÐ´Ð¸ ÑÐ¾ÑÐ¾Ñ ÑÐ¾Ð¼Ð½ÑÑ Ð°Ð¼Ð¸Ð½Ð¾Ð²
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Search for molecular switches of glutamate and anatropic AMPA and NMDA type receptors among photochromic amines
Creators: name: Ð®Ð¿Ð°ÑÐµÐ², ÐÐ»Ð°Ð´Ð¸Ð¼Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: AMPA ÑÐµÑÐµÐ¿ÑÐ¾ÑÑ
NMDA ÑÐµÑÐµÐ¿ÑÐ¾ÑÑ
Ð³Ð»ÑÑÐ°Ð¼Ð°Ñ
Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
Ð¿ÑÑÑ-ÐºÐ»Ð°Ð¼Ð¿
DENAQ
ÑÐ¾ÑÐ¾Ñ ÑÐ¾Ð¼Ð½ÑÐµ Ð¼Ð¾Ð»ÐµÐºÑÐ»Ñ
AMPA receptors
NMDA receptors
glutamare
inhibition
patch clamp
photochromic molecules

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«ÐÐ¾Ð¸ÑÐº Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½ÑÑ Ð¿ÐµÑÐµÐºÐ»ÑÑÐ°ÑÐµÐ»ÐµÐ¹ Ð³Ð»ÑÑÐ°Ð¼Ð°ÑÐ½ÑÑ Ð¸ Ð°Ð½Ð°ÑÑÐ¾Ð¿Ð½ÑÑ ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð² AMPA Ð¸ NMDA ÑÐ¸Ð¿Ð° ÑÑÐµÐ´Ð¸ ÑÐ¾ÑÐ¾ÑÑÐ¾Ð¼Ð½ÑÑ Ð°Ð¼Ð¸Ð½Ð¾Ð²Â».Â ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ ÑÐµÑÐ¸Ð¸ Ð°Ð·Ð¾Ð±ÐµÐ½Ð·Ð¾Ð»ÑÐ¾Ð´ÐµÑÐ¶Ð°ÑÐ¸Ñ Ð°Ð¼Ð¸Ð½Ð¾Ð² Ð½Ð° AMPA Ð¸ NMDA Ð¸Ð¾Ð½Ð¾ÑÑÐ¾Ð¿Ð½ÑÐµ Ð³Ð»ÑÑÐ°Ð¼Ð°ÑÐ½ÑÐµ ÑÐµÑÐµÐ¿ÑÐ¾ÑÑ. ÐÑÐ¾ÑÐ¾ÑÐ¸Ð¿Ð¾Ð¼ ÑÑÐ¸Ñ Ð²ÐµÑÐµÑÑÐ² ÑÐ²Ð»ÑÐµÑÑÑ ÑÐ¾ÐµÐ´Ð¸Ð½ÐµÐ½Ð¸Ðµ DENAQ â Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´Ð½Ð¾Ðµ ÑÐµÑÐ²ÐµÑÑÐ¸ÑÐ½Ð¾Ð³Ð¾ Ð°Ð¼Ð¼Ð¾Ð½Ð¸Ñ, ÐºÐ¾ÑÐ¾ÑÐ¾Ðµ ÑÐ²Ð»ÑÐµÑÑÑ ÑÑÑÐµÐºÑÐ¸Ð²Ð½ÑÐ¼ ÑÐ²ÐµÑÐ¾ÑÑÐ²ÑÑÐ²Ð¸ÑÐµÐ»ÑÐ½ÑÐ¼ Ð°Ð½ÑÐ°Ð³Ð¾Ð½Ð¸ÑÑÐ¾Ð¼ NMDA ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð².Â Ð ÑÐ°Ð±Ð¾ÑÐµ Ð±ÑÐ»Ð° Ð¾ÑÐµÐ½ÐµÐ½Ð° ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ ÑÐ¾ÐµÐ´Ð¸Ð½ÐµÐ½Ð¸Ð¹ Ð¼Ð¾Ð´ÑÐ»Ð¸ÑÐ¾Ð²Ð°ÑÑ ÑÑÐ°Ð½ÑÐ¼ÐµÐ¼Ð±ÑÐ°Ð½Ð½ÑÐµ ÑÐ¾ÐºÐ¸ ÑÐµÑÐµÐ· Ð³Ð»ÑÑÐ°Ð¼Ð°ÑÐ½ÑÐµ ÑÐµÑÐµÐ¿ÑÐ¾ÑÑ, Ð° ÑÐ°ÐºÐ¶Ðµ ÑÐ²ÐµÑÐ¾Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾ÑÑÑ Ð¸Ñ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ. ÐÐºÐ°Ð·Ð°Ð»Ð¾ÑÑ, ÑÑÐ¾ Ð´Ð°Ð½Ð½ÑÐµ Ð²ÐµÑÐµÑÑÐ²Ð° Ð¿ÑÐµÐ¸Ð¼ÑÑÐµÑÑÐ²ÐµÐ½Ð½Ð¾ ÑÐ³Ð½ÐµÑÐ°ÑÑ NMDA ÑÐµÑÐµÐ¿ÑÐ¾ÑÑ. Ð ÑÑÐ»Ð¾Ð²Ð¸ÑÑ Ð¼Ð¾Ð½Ð¾ÑÑÐ¾Ð¼Ð°ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐ²ÐµÑÐ° Ð´ÐµÐ¹ÑÑÐ²Ð¸Ðµ ÑÐ¾ÐµÐ´Ð¸Ð½ÐµÐ½Ð¸Ð¹ Ð¾ÑÐ»Ð°Ð±ÐµÐ²Ð°ÐµÑ. ÐÑÑÐ²Ð»ÐµÐ½Ñ Ð½ÐµÐºÐ¾ÑÐ¾ÑÑÐµ ÑÑÑÑÐºÑÑÑÐ½ÑÐµ Ð´ÐµÑÐµÑÐ¼Ð¸Ð½Ð°Ð½ÑÑ, Ð¾ÑÐ²ÐµÑÑÑÐ²ÐµÐ½Ð½ÑÐµ Ð·Ð° ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ðµ ÑÐ³Ð½ÐµÑÐ°ÑÑÐµÐ³Ð¾ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ. ÐÐ° AMPA ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ°Ñ Ð²ÐµÑÐµÑÑÐ²Ð° Ð¿ÑÐ¾ÑÐ²Ð»ÑÑÑ ÑÐ»Ð°Ð±ÑÑ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ. ÐÐ»Ñ Ð²ÐµÑÐµÑÑÐ²Ð°, Ð¾ÑÐ»Ð¸ÑÐ°ÑÑÐµÐ³Ð¾ÑÑ Ð½Ðµ ÑÐ°ÑÐ°ÐºÑÐµÑÐ½Ð¾Ð¹ Ð´Ð»Ñ Ð±Ð¾Ð»ÑÑÐ¸Ð½ÑÑÐ²Ð° ÑÐ¾ÐµÐ´Ð¸Ð½ÐµÐ½Ð¸Ð¹ Ð¼ÐµÐ´Ð»ÐµÐ½Ð½Ð¾Ð¹ ÐºÐ¸Ð½ÐµÑÐ¸ÐºÐ¾Ð¹ ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ñ ÑÑÑÐµÐºÑÐ¾Ð², Ð±ÑÐ»Ð° Ð¿Ð¾Ð´ÑÐ¾Ð±Ð½Ð¾ Ð¸Ð·ÑÑÐµÐ½Ð° ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾ÑÑÑ Ð¸ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð¾Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾ÑÑÑ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ.Â ÐÑÐ½Ð¾Ð²Ð½ÑÐµ Ð¼ÐµÑÐ¾Ð´Ñ, Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð½ÑÐµ Ð² Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ: Ð¿ÑÑÑ-ÐºÐ»Ð°Ð¼Ð¿ ÑÐµÐ³Ð¸ÑÑÑÐ°ÑÐ¸Ñ Ð°Ð³Ð¾Ð½Ð¸ÑÑ-Ð²ÑÐ·Ð²Ð°Ð½Ð½ÑÑ ÑÐ¾ÐºÐ¾Ð² Ð² ÑÐµÐ¶Ð¸Ð¼Ðµ ÑÐ¸ÐºÑÐ°ÑÐ¸Ð¸ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ð° Ð¸ Ð² ÐºÐ¾Ð½ÑÐ¸Ð³ÑÑÐ°ÑÐ¸Ð¸ Â«ÑÐµÐ»Ð°Ñ ÐºÐ»ÐµÑÐºÐ°Â», Ð¿ÑÐ¸Ð³Ð¾ÑÐ¾Ð²Ð»ÐµÐ½Ð¸Ðµ Ð¿ÐµÑÐµÐ¶Ð¸Ð²Ð°ÑÑÐ¸Ñ ÑÑÐµÐ·Ð¾Ð² Ð¼Ð¾Ð·Ð³Ð°, Ð²Ð¸Ð±ÑÐ¾Ð´Ð¸ÑÑÐ¾ÑÐ¸Ð°ÑÐ¸Ñ Ð½ÐµÐ¹ÑÐ¾Ð½Ð¾Ð² Ð¸Ð· ÑÑÐµÐ·Ð¾Ð², Ð¿Ð¾Ð´Ð°ÑÐ° Ð¸ÑÑÐ»ÐµÐ´ÑÐµÐ¼ÑÑ Ð²ÐµÑÐµÑÑÐ² Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÑÐ¸ÑÑÐµÐ¼Ñ Ð±ÑÑÑÑÐ¾Ð¹ Ð°Ð¿Ð¿Ð»Ð¸ÐºÐ°ÑÐ¸Ð¸.
description: Topic of the final qualifying work: "Search for molecular switches of glutamate and anatropic AMPA and NMDA type receptors among photochromic amines". This work is devoted to the study of the action of a series of azobenzenecontaining amines on AMPA and NMDA ionotropic glutamate receptors. The prototype of these substances is the DENAQ compound, a quaternary ammonium derivative, which is an effective photosensitive NMDA receptor antagonist. The ability of the compounds to modulate transmembrane currents through glutamate receptors, as well as the light dependence of their action, was evaluated in the work. It turned out that these substances predominantly inhibit NMDA receptors. Under conditions of monochromatic light, the action of the compounds weakens. Some structural determinants responsible for the development of the inhibitory effect have been identified. Substances show weak activity on AMPA receptors. For a substance that is characterized by a slow kinetics of the development of effects, which is not characteristic of most compounds, the concentration dependence and potential dependence of the action were studied in detail. The main methods used in this work were: patch-clamp recording of agonistinduced currents in the potential-clamp mode and in the âwhole cellâ configuration, preparation of surviving brain slices, vibrodissociation of neurons from slices, delivery of test substances using a rapid application system.