title: ÐÐ·ÑÑÐµÐ½Ð¸Ðµ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÐ¸ ÐºÐ°Ð»Ð»ÑÑÐ¾Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¾Ð²Ð¾ÑÐ½ÑÑ ÐºÑÐ»ÑÑÑÑ Cucumis sativus L. Ð¸ Beta vulgaris L.
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Studying the ability of callus formation of vegetable crops Cucumis sativus L. and Beta vulgaris L.
Creators: name: ÐÐ°Ð¿ÐºÐ°ÑÐ¾Ð², ÐÐ¸ÑÐ°Ð¸Ð»
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: ÐºÐ°Ð»Ð»ÑÑ
ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÑÐ°ÑÑÐµÐ½Ð¸Ð¹
ÑÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸Ñ ÑÐ°ÑÑÐµÐ½Ð¸Ð¹
callus
plant cultivation
plant regeneration

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ ÑÑÐ»Ð¾Ð²Ð¸Ð¹, Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÑÑÐ¸Ñ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð½Ð° Ð¿ÑÐ¾ÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ðµ ÑÐµÐ¼ÑÐ½ ÑÐ¾ÑÑÐ¾Ð² Ð¾Ð²Ð¾ÑÐ½ÑÑ ÐºÑÐ»ÑÑÑÑ Ð¸ Ð½Ð° Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ðµ ÐºÐ°Ð»Ð»ÑÑÐ° Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ in vitro. ÐÐ°Ð´Ð°ÑÐ¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: â Ð¿Ð¾Ð´Ð¾Ð±ÑÐ°ÑÑ Ð¼ÐµÑÐ¾Ð´Ñ ÑÑÐµÑÐ¸Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð´Ð»Ñ ÑÐµÐ¼ÑÐ½ Ð¸Ð·ÑÑÐ°ÐµÐ¼ÑÑ ÐºÑÐ»ÑÑÑÑ; â Ð¾Ð¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ ÑÑÐ»Ð¾Ð²Ð¸Ñ Ð¸ ÑÐ¾ÑÑÐ°Ð² Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ´Ñ Ð´Ð»Ñ Ð²Ð²ÐµÐ´ÐµÐ½Ð¸Ñ ÑÐµÐ¼ÑÐ½ Ð² ÐºÑÐ»ÑÑÑÑÑ in vitro; â Ð¿Ð¾Ð´Ð¾Ð±ÑÐ°ÑÑ ÑÐ¾ÑÑÐ°Ð² Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ´Ñ Ð¸ ÑÑÐ»Ð¾Ð²Ð¸Ñ Ð´Ð»Ñ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ ÐºÐ°Ð»Ð»ÑÑÐ½Ð¾Ð¹ ÑÐºÐ°Ð½Ð¸ Ð¸Ð·ÑÑÐ°ÐµÐ¼ÑÑ ÑÐ¾ÑÑÐ¾Ð²; â Ð¿ÑÐ¾Ð²ÐµÑÑÐ¸ ÑÑÐ°Ð²Ð½Ð¸ÑÐµÐ»ÑÐ½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð· Ð¼Ð¾ÑÑÐ¾ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð¾ÑÐ¾Ð±ÐµÐ½Ð½Ð¾ÑÑÐµÐ¹ Ð¿Ð¾Ð»ÑÑÐ°ÐµÐ¼ÑÑ ÐºÐ°Ð»Ð»ÑÑÐ¾Ð². Ð Ð°Ð±Ð¾ÑÐ° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð² ÑÐµÐ½ÑÑÐµ ÐºÐ¾Ð»Ð»ÐµÐºÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ Ð¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ñ Â«ÐÐ°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ñ Ð¸ÑÐºÑÑÑÑÐ²ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð²ÑÑÐ°ÑÐ¸Ð²Ð°Ð½Ð¸Ñ Ð¸ Ð¾Ð·Ð´Ð¾ÑÐ¾Ð²Ð»ÐµÐ½Ð¸Ñ Ð³ÐµÐ½Ð¾ÑÐ¾Ð½Ð´Ð° ÑÐ°ÑÑÐµÐ½Ð¸Ð¹Â» Ð½Ð° Ð±Ð°Ð·Ðµ Ð¤ÐµÐ´ÐµÑÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð³Ð¾ÑÑÐ´Ð°ÑÑÑÐ²ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð±ÑÐ´Ð¶ÐµÑÐ½Ð¾Ð³Ð¾ Ð½Ð°ÑÑÐ½Ð¾Ð³Ð¾ ÑÑÑÐµÐ¶Ð´ÐµÐ½Ð¸Ñ Â«Ð¤ÐµÐ´ÐµÑÐ°Ð»ÑÐ½ÑÐ¹ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÑÐºÐ¸Ð¹ ÑÐµÐ½ÑÑ ÐÑÐµÑÐ¾ÑÑÐ¸Ð¹ÑÐºÐ¸Ð¹ Ð¸Ð½ÑÑÐ¸ÑÑÑ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÐµÑÑÑÑÐ¾Ð² ÑÐ°ÑÑÐµÐ½Ð¸Ð¹ Ð¸Ð¼ÐµÐ½Ð¸ Ð.Ð. ÐÐ°Ð²Ð¸Ð»Ð¾Ð²Ð°Â»,Â  Ð° ÑÐ°ÐºÐ¶Ðµ ÑÐ°ÑÑÑ ÑÐ°Ð±Ð¾ÑÑ Ð²ÑÐ¿Ð¾Ð»Ð½ÑÐ»Ð°ÑÑ Ð½Ð° Ð±Ð°Ð·Ðµ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ ÐÐ¨ÐÐ¸ÐÐ Ð¡ÐÐ±ÐÐ£. ÐÑÐ»Ð¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ñ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÑ Ð¿Ð¾ ÑÑÐµÑÐ¸Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ ÑÐµÐ¼ÑÐ½ ÑÐ¾ÑÑÐ¾Ð² Cucumis sativus L. Ð¸ BetaÂ  vulgaris L. Ð¸ Ð²Ð²ÐµÐ´ÐµÐ½Ð¸Ð¸ Ð¸Ñ Ð² ÐºÑÐ»ÑÑÑÑÑ in vitro. Ð ÑÐ¾Ð´Ðµ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¸ Ð¿Ð¾Ð´Ð¾Ð±ÑÐ°Ð½Ñ Ð¾Ð¿ÑÐ¸Ð¼Ð°Ð»ÑÐ½ÑÐµ ÑÑÐ»Ð¾Ð²Ð¸Ñ ÑÑÐµÑÐ¸Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ ÑÐµÐ¼ÑÐ½ Ð¸Ð·ÑÑÐ°ÐµÐ¼ÑÑ Ð¾Ð²Ð¾ÑÐ½ÑÑ ÐºÑÐ»ÑÑÑÑ, Ð¿Ð¾Ð´Ð¾Ð±ÑÐ°Ð½Ñ ÑÑÐµÐ´Ñ Ð¸ ÑÑÐ»Ð¾Ð²Ð¸Ñ Ð²Ð²ÐµÐ´ÐµÐ½Ð¸Ñ ÑÐµÐ¼ÑÐ½ Ð² ÐºÑÐ»ÑÑÑÑÑ in vitro, Ð±ÑÐ» Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ½ Ð¸ Ð¸Ð·ÑÑÐµÐ½ Ð¿ÑÐ¾ÑÐµÑÑ Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ñ ÐºÐ°Ð»Ð»ÑÑÐ° Ð½Ð° ÑÐºÑÐ¿Ð»Ð°Ð½ÑÐ°Ñ ÑÐ°Ð·Ð»Ð¸ÑÐ½Ð¾Ð³Ð¾ Ð¿ÑÐ¾Ð¸ÑÑÐ¾Ð¶Ð´ÐµÐ½Ð¸Ñ. Ð Ð°ÑÑÐ¼Ð¾ÑÑÐµÐ½Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¹ ÑÑÐµÐ´Ñ ÐÑÑÐ°ÑÐ¸Ð³Ðµ-Ð¡ÐºÑÐ³Ð° Ð½Ð° Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ðµ ÐºÐ°Ð»Ð»ÑÑÐ½Ð¾Ð¹ ÑÐºÐ°Ð½Ð¸. ÐÐ¿Ð¸ÑÐ°Ð½Ñ Ð¼Ð¾ÑÑÐ¾ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ¸ ÐºÐ°Ð»Ð»ÑÑÐ½ÑÑ ÑÐºÐ°Ð½ÐµÐ¹. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐ¹ Ð¿Ð¾ Ð¸ÑÐ¾Ð³Ñ ÑÐ°Ð±Ð¾ÑÑ ÐºÐ°Ð»Ð»ÑÑ Ð¼Ð¾Ð¶Ð½Ð¾ Ð¿ÑÐ¸Ð¼ÐµÐ½ÑÑÑ Ð´Ð»Ñ Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ ÑÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÐ¸ ÑÑÐ¸Ñ Ð¾Ð²Ð¾ÑÐ½ÑÑ ÐºÑÐ»ÑÑÑÑ, Ð´Ð»Ñ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð² ÑÐ¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð¹ ÑÑÐºÐ¾ÑÐµÐ½Ð½Ð¾Ð¹ ÑÐµÐ»ÐµÐºÑÐ¸Ð¸, Ð² ÑÐ¾Ð¼ ÑÐ¸ÑÐ»Ðµ ÑÐµÐ»ÐµÐºÑÐ¸Ð¸ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐµÐ´Ð°ÐºÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ. ÐÐ»Ñ Ð½Ð°Ð¿Ð¸ÑÐ°Ð½Ð¸Ñ ÑÐ°Ð±Ð¾ÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ¾Ð²ÑÐµÐ¼ÐµÐ½Ð½ÑÐµ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ Ð¾ÑÐµÑÐµÑÑÐ²ÐµÐ½Ð½Ð¾Ð¹ Ð¸ Ð·Ð°ÑÑÐ±ÐµÐ¶Ð½Ð¾Ð¹ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÑ: ÐLIBRARY, Scopus, ÐÐºÐ°Ð´ÐµÐ¼Ð¸Ñ Google, ÐÐ¸Ð±ÐµÑÐ»ÐµÐ½Ð¸Ð½ÐºÐ°, NSBI, Ð² Ð¾Ð±Ð»Ð°ÑÑÐ¸ Ð±Ð¸Ð¾ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¸ ÑÐ°ÑÑÐµÐ½Ð¸Ð¹ Ð¸ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÑÐ°ÑÑÐ¸ÑÐµÐ»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ ÐºÑÐ»ÑÑÑÑ: Plant, AgroAtlas, Plantarium, Kew, GBIF, Ñ ÑÑÐµÑÐ¾Ð¼ Ð¾ÑÐ½Ð¾Ð²Ð½ÑÑ ÑÑÐµÐ±Ð¾Ð²Ð°Ð½Ð¸Ð¹ Ð¸Ð½ÑÐ¾ÑÐ¼Ð°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ Ð±ÐµÐ·Ð¾Ð¿Ð°ÑÐ½Ð¾ÑÑÐ¸. ÐÐ»Ñ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð¸ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÐ¸ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð² Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ñ: Microsoft Excel, STATISTICA, Aspose, Biorender, Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼Ñ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÐ¸ Ð¼Ð¸ÐºÑÐ¾ÑÐ¾ÑÐ¾Ð³ÑÐ°ÑÐ¸Ð¹ ÐÐ¡-foto Ð¸ Levenhuk.
description: This work is devoted to the study of conditions affecting the germination of seeds of vegetable varieties and the formation of callus under in vitro cultivation. Research objectives: â to select sterilization methods for seeds of the studied crops; â to select the conditions and composition of the nutrient medium for the introduction of seeds into culture in vitro; â to select the composition of the nutrient medium and conditions for obtaining the callus tissue of the studied varieties; â to conduct a comparative analysis of the morphophysiological features of the resulting calluses. The work was carried out in the center for collective use "Laboratory of artificial cultivation and improvement of the plant gene pool" on the basis of the Federal State Budgetary Scientific Institution "Federal Research Center All-Russian Institute of Plant Genetic Resources named after N.I. Vavilov", and part of the work was carried out on the basis of the laboratory of the HSE SPbPU. Experiments were conducted to sterilize seeds of Cucumis sativus L. and Beta vulgaris L. varieties and introduce them into culture in vitro. In the course of the work, optimal conditions for the sterilization of seeds of the studied vegetable crops were selected, media and conditions for the introduction of seeds into culture in vitro were selected, the process of callus formation on explants of various origins was carried out and studied. The influence of various modifications of the Murashige-Skuga medium on the formation of callus tissue is considered. Morphophysiological characteristics of callus tissues are described. The resulting callus can be used to study the regenerative ability of these vegetable crops, for use in modern accelerated breeding, including breeding using genetic editing methods. To write the work, modern databases of domestic and foreign literature were used: LIBRARY, Scopus, Google Academy, Cyberleninka, NSBI, in the field of plant biotechnology and cultivation of plant cell cultures: Plant, AgroAtlas, Plantarium, Kew, GBIF, taking into account the basic requirements of information security. For the analysis and processing of the results, the following programs were used: Microsoft Excel, STATISTICA, Aspose, Biorender, microphotography processing programs MS-foto and Levenhuk.