title: Ð Ð¾Ð»Ñ Ð»ÐµÐºÑÐ¸Ð½Ð¾Ð² C-ÑÐ¸Ð¿Ð° Ð² ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ð¸ Ð³ÑÐ¸Ð¿Ð¿Ð¾Ð·Ð½Ð¾Ð¹ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸ in vitro
title: Ð¼Ð°Ð³Ð¸ÑÑÐµÑÑÐºÐ°Ñ Ð´Ð¸ÑÑÐµÑÑÐ°ÑÐ¸Ñ
titleType: Subtitle
Creators: name: ÐÐ³Ð¾ÑÐ¾Ð²Ð°, ÐÐ½Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2016
Subjects: ÐÐ»ÐµÑÐºÐ° (Ð±Ð¸Ð¾Ð»)
Ð»ÐµÐºÑÐ¸Ð½Ñ C-ÑÐ¸Ð¿Ð°
Ð²Ð¸ÑÑÑ Ð³ÑÐ¸Ð¿Ð¿Ð° A
Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð½Ð°Ñ ÑÐµÐ¿Ð½Ð°Ñ ÑÐµÐ°ÐºÑÐ¸Ñ
C-type lectins
influenza virus A
polymerase chain reaction

description: ÐÐ°ÑÑÐ¾ÑÑÐ°Ñ Ð¼Ð°Ð³Ð¸ÑÑÐµÑÑÐºÐ°Ñ Ð´Ð¸ÑÑÐµÑÑÐ°ÑÐ¸Ñ Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ ÑÐ¾Ð»Ð¸ Ð»ÐµÐºÑÐ¸Ð½Ð° C-ÑÐ¸Ð¿Ð° DC-SIGN Ð² ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ð¸ Ð³ÑÐ¸Ð¿Ð¿Ð¾Ð·Ð½Ð¾Ð¹ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸. Ð ÑÐ°Ð±Ð¾ÑÐµ Ð¿ÑÐ¸Ð²ÐµÐ´ÐµÐ½ Ð¾Ð±Ð·Ð¾Ñ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¾ ÑÑÑÐ¾ÐµÐ½Ð¸Ð¸, Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð¼Ð°, Ð¶Ð¸Ð·Ð½ÐµÐ½Ð½Ð¾Ð¼ ÑÐ¸ÐºÐ»Ðµ Ð²Ð¸ÑÑÑÐ° Ð³ÑÐ¸Ð¿Ð¿Ð°, Ð¿ÑÐ¸Ð²ÐµÐ´ÐµÐ½Ð° Ð¾Ð±ÑÐ°Ñ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ° Ð»ÐµÐºÑÐ¸Ð½Ð¾Ð² Ð¡-ÑÐ¸Ð¿Ð°, ÑÐ°ÑÑÐ¼Ð¾ÑÑÐµÐ½Ð° ÑÐ¾Ð»Ñ Ð»ÐµÐºÑÐ¸Ð½Ð° DC-SIGN Ð² Ð³ÑÐ¸Ð¿Ð¿Ð¾Ð·Ð½Ð¾Ð¹ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸. Ð ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ» ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½ Ð¸ ÑÑÐ¿ÐµÑÐ½Ð¾ Ð²Ð½ÐµÐ´ÑÐµÐ½ Ð² Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ½ÑÑ Ð¿ÑÐ°ÐºÑÐ¸ÐºÑ Ð¿ÑÐ¾ÑÐ¾ÐºÐ¾Ð» ÑÐµÐºÐ²ÐµÐ½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¿Ð¾Ð»Ð½Ð¾Ð¹ Ð½ÑÐºÐ»ÐµÐ¾ÑÐ¸Ð´Ð½Ð¾Ð¹ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÐ¸ Ð³ÐµÐ½Ð° DC-SIGN ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°, ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð° ÑÐ¸ÑÑÐµÐ¼Ð° Ð¿ÑÐ°Ð¹Ð¼ÐµÑÐ¾Ð² Ð´Ð»Ñ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð¼Ð ÐÐ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ Ð¸Ð·Ð¾ÑÐ¾ÑÐ¼ DC-SIGN Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ ÐÐ¦Ð  Ð² ÑÐµÐ°Ð»ÑÐ½Ð¾Ð¼ Ð²ÑÐµÐ¼ÐµÐ½Ð¸ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¸Ð½ÑÐµÑÐºÐ°Ð»Ð¸ÑÑÑÑÐµÐ³Ð¾ ÐºÑÐ°ÑÐ¸ÑÐµÐ»Ñ SYBR Green. Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð½Ð°Ñ ÑÐ¸ÑÑÐµÐ¼Ð° Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÐµÑ ÑÐ°Ð·Ð»Ð¸ÑÐ°ÑÑ Ð¼Ð ÐÐ Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ð½ÑÑ Ð¸ ÑÐ°ÑÑÐ²Ð¾ÑÐ¸Ð¼ÑÑ Ð¸Ð·Ð¾ÑÐ¾ÑÐ¼ DC-SIGN, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¼Ð ÐÐ Ð¸Ð·Ð¾ÑÐ¾ÑÐ¼ ÑÐ¸Ð¿Ð¾Ð² Ð Ð¸ B. ÐÑÐ¸ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½Ð¾Ð¼ Ð·Ð°ÑÐ°Ð¶ÐµÐ½Ð¸Ð¸ Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð²Ð¸ÑÑÑÐ°Ð¼Ð¸ Ð³ÑÐ¸Ð¿Ð¿Ð° Ð Ð²ÑÑÐ²Ð»ÐµÐ½Ð¾ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ðµ ÑÑÐ¾Ð²Ð½Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð¼Ð ÐÐ ÑÑÐ°Ð½ÑÐ¼ÐµÐ¼Ð±ÑÐ°Ð½Ð½ÑÑ Ð¸Ð·Ð¾ÑÐ¾ÑÐ¼ DC-SIGN Ð² 50 ÑÐ°Ð·.
description: This master thesis is devoted to the study the role of C-type lectin DC-SIGN in the progress of influenza virus infection. This work includes review of the literature on structure, organization of influenza genome, lifecycle of the influenza virus, general description of the C-type lectins and possible role of DC-SIGN in the course of influenza virus infection is discussed. Protocol of sequencing of the full-length nucleotide sequence of human DC-SIGN gene was developed and successfully introduced into laboratory practice. Also the real-time PCR assay for the analysis of mRNA expression of various DC-SIGN isoforms was developed. This assay can distinguish mRNAs encoding soluble and membrane isoforms of DC-SIGN and mRNAs of types A and B isoforms. Analysis of DC-SIGN mRNA expression by developed RT-PCR assay showed 50-fold decrease in mRNA level of membrane isoforms in dendritic cells infected by influenza A viruses.