Additional file 1 of Minos and Restless transposon insertion mutagenesis of psychrotrophic fungus for red pigment synthesis adaptive to normal temperature
Creators: name: Lu, Fengning
name: Ren, Yanna
name: Ding, Lulu
name: Lu, Jian
name: Zhou, Xiangshan
name: Liu, Haifeng
name: Wang, Nengfei
name: Cai, Menghao
Publisher: name: figshare
Year: 2022
Subjects: subject: Space Science
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Genetics
subject: Molecular Biology
subject: Biotechnology
subject: Ecology
subject: Biological Sciences not elsewhere classified
subject: Developmental Biology

description: Additional file 1: Table S1. Primers used in this study. Figure S1. Mutants screened by single transposable systems of Minos and Restless. (a) Cultured at 20℃. Right in each plate: wild-type (WT); Left in each plate: mutants (MPS1 ~ MPS10). MPS1 ~ MPS7, screened by Minos transposon; MPS8 ~ MPS10, screened by Restless transposon. (b) Cultured at 25 °C. WT, wild-type; MGS1 ~ MGS4, screened by Minos transposon; MGS5 ~ MGS6, screened by Restless transposon. * indicates a significant difference at p &lt; 0.05, ** indicates a significant difference at p &lt; 0.01. The pictures in figure were gained from different incubation time. The mutants were inoculated with the same number of spores as the wild-type. Figure S2. The red pigment production of mutants obtained by single transposable systems in fermentation. (a) Cultured at 14℃. (b) Cultured at 20℃. WT, wild-type; mutants MPS1 ~ MPS10, screened by single transposable systems; DW, biomass dry weight. Determination of absorbance value of red pigment at wavelength of 520 nm (OD520). Figure S3. The growth of mutants obtained by single transposable systems in fermentation at 25 °C. WT, wild-type; (a) MGS1. (b) MGS3. (c) MGS6, screened by single transposable systems; DW, biomass dry weight. Figure S4. The varying amplitude analysis of production and growth of positive mutants in liquid culture. The variation percentage in red pigment production of mutants cultured at 14 °C (a) and 20 °C (c) were shown. The variation percentage in cell growth of mutants cultured at 14 °C (b), 20 °C (d), and 25 °C (e) were shown. WT, wild-type; mutants MPS1 ~ MPS10, MGS1, MGS3 and MGS 6 screened by single transposable systems. Figure S5. Mutants screened by dual transposable systems of Minos and Restless. (a) Cultured at 20℃. Right in each plate, wild-type (WT); Left in each plate: mutants (MPD1 ~ MPD13). MPD1 ~ MPD6, screened by Minos transposon; MPD7 ~ MPD13, screened by Restless transposon. (b) Cultured at 25 °C. Mutants screened by dual transposable systems. WT, wild-type; MGD1 ~ MGD4, screened by Minos transposon; MGD5 ~ MGD8, screened by Restless transposon. * indicates a significant difference at p &lt; 0.05, ** indicates a significant difference at p &lt; 0.01. The pictures in figure were gained from different incubation time. The mutants were inoculated with the same number of spores as the wild-type. Figure S6. The red pigment production of mutants obtained by dual transposable systems in fermentation. a (a) Cultured at 14 °C. (b) Cultured at 20 °C. WT, wild-type; mutants MPD1 ~ MPD13, screened by dual transposable systems; DW, biomass dry weight. Determination of absorbance value of red pigment at wavelength of 520 nm (OD520). Figure S7. The growth of mutants obtained by dual transposable systems in fermentation at 25˚C. WT, wild-type; (a) MGD2. (b) MGD4. (c) MGD6, screened by dual transposable systems; DW, biomass dry weight. Figure S8. The varying amplitude analysis of production and growth of positive mutants in liquid culture. The variation percentage in red pigment production of mutants cultured at 14 °C (a) and 20 °C (c) were shown. The variation percentage in cell growth of mutants cultured at 14 °C (b), 20 °C (d), and 25 °C (e) were shown. WT, wild-type; mutants MPD1 ~ MPD13, MGD2, MGD4 and MGD6 screened by dual transposable systems.