title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ Ð½ÑÐºÐ»ÐµÐ¾Ð¿ÑÐ¾ÑÐµÐ¸Ð½Ð° Ð²Ð¸ÑÑÑÐ° Ð³ÑÐ¸Ð¿Ð¿Ð° Ð½Ð° ÑÐ¾ÑÑÐ¾Ð²ÑÐµ Ñ Ð°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ¸ Ð¶Ð¸Ð²Ð¾Ð¹ Ð³ÑÐ¸Ð¿Ð¿Ð¾Ð·Ð½Ð¾Ð¹ Ð²Ð°ÐºÑÐ¸Ð½Ñ
title: Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÑÐºÐ°Ñ ÑÐ°Ð±Ð¾ÑÐ°
titleType: Subtitle
Creators: name: Ð¥Ð°Ð¹ÑÑÑÐµÐ²Ð°, Ð­Ð»ÑÐ¼Ð¸ÑÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2016
Subjects: Ð¶Ð¸Ð²Ð°Ñ Ð³ÑÐ¸Ð¿Ð¿Ð¾Ð·Ð½Ð°Ñ Ð²Ð°ÐºÑÐ¸Ð½Ð°
ÑÐµÐ°ÑÑÐ¾ÑÑÐ°Ð½ÑÐ½ÑÐ¹ ÑÑÐ°Ð¼Ð¼
Ð½ÑÐºÐ»ÐµÐ¾Ð¿ÑÐ¾ÑÐµÐ¸Ð½ Ð²Ð¸ÑÑÑÐ° Ð³ÑÐ¸Ð¿Ð¿Ð°
ÑÐ°Ð·Ð²Ð¸Ð²Ð°ÑÑÐ¸ÐµÑÑ ÐºÑÑÐ¸Ð½ÑÐµ ÑÐ¼Ð±ÑÐ¸Ð¾Ð½Ñ
ÐºÑÐ»ÑÑÑÑÐ° ÐºÐ»ÐµÑÐ¾Ðº MDCK
live attenuated influenza vaccine
reassortant strain
nucleoprotein of influenza virus
developing chicken embryos
cell culture MDCK

description: Ð Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ Ð±ÑÐ»Ð° Ð¸Ð·ÑÑÐµÐ½Ð° ÑÐµÐ¿ÑÐ¾Ð´ÑÐºÑÐ¸Ð²Ð½Ð°Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÑÐµÐ°ÑÑÐ¾ÑÑÐ°Ð½ÑÐ½ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð² Ð²Ð¸ÑÑÑÐ° Ð³ÑÐ¸Ð¿Ð¿Ð°, ÑÐ¾Ð´ÐµÑÐ¶Ð°ÑÐ¸Ñ Ð½ÑÐºÐ»ÐµÐ¾Ð¿ÑÐ¾ÑÐµÐ¸Ð½ (NP) Ð¾Ñ Ð²Ð¸ÑÑÑÐ¾Ð² ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ Ð»ÐµÑ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ. Ð ÑÐ¾Ð´Ðµ Ð¿Ð°ÑÐ½Ð¾Ð³Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ Ð²Ð°ÐºÑÐ¸Ð½Ð½ÑÑ ÑÑÐ°Ð¼Ð¼Ð¾Ð² Ð¶Ð¸Ð²Ð¾Ð¹ Ð³ÑÐ¸Ð¿Ð¿Ð¾Ð·Ð½Ð¾Ð¹ Ð²Ð°ÐºÑÐ¸Ð½Ñ (ÐÐÐ), ÑÐ¾Ð´ÐµÑÐ¶Ð°ÑÐ¸Ñ NP Ð¾Ñ ÑÑÑÐ°ÑÐµÐ²ÑÐµÐ³Ð¾ ÑÑÐ°Ð¼Ð¼Ð° - Ð´Ð¾Ð½Ð¾ÑÐ° Ð°ÑÑÐµÐ½ÑÐ°ÑÐ¸Ð¸ (ÑÐ¾ÑÐ¼ÑÐ»Ð° Ð³ÐµÐ½Ð¾Ð¼Ð° 6:2) Ð¸ Ð¾Ñ ÑÐ¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð²Ð¸ÑÑÑÐ° H1N1 Ð¸Ð»Ð¸ H7N9 (ÑÐ¾ÑÐ¼ÑÐ»Ð° Ð³ÐµÐ½Ð¾Ð¼Ð° 5:3) Ð²ÑÑÐ²Ð»ÐµÐ½Ð¾ ÑÐ°Ð·Ð½Ð¾Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð½Ð¾Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ NP Ð³ÐµÐ½Ð° Ð½Ð° ÑÐ¾ÑÑÐ¾Ð²ÑÐµ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ¸ ÐÐÐ. ÐÐ»Ñ Ð²Ð¸ÑÑÑÐ° Ð¿Ð¾Ð´ÑÐ¸Ð¿Ð° H1N1 Ð±ÑÐ»Ð¾ ÑÐ²Ð¾Ð¹ÑÑÐ²ÐµÐ½Ð½Ð¾ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ðµ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÑÐµÐ¿ÑÐ¾Ð´ÑÐºÑÐ¸Ð¸ 5:3 ÑÐµÐ°ÑÑÐ¾ÑÑÐ°Ð½ÑÐ° Ð¿ÑÐ¸ Ð¾Ð¿ÑÐ¸Ð¼Ð°Ð»ÑÐ½Ð¾Ð¹ ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÐµ Ð¸Ð½ÐºÑÐ±Ð°ÑÐ¸Ð¸, ÐºÐ°Ðº Ð² ÑÐ°Ð·Ð²Ð¸Ð²Ð°ÑÑÐ¸ÑÑÑ ÐºÑÑÐ¸Ð½ÑÑ ÑÐ¼Ð±ÑÐ¸Ð¾Ð½Ð°Ñ (Ð ÐÐ­), ÑÐ°Ðº Ð¸ Ð² ÐºÑÐ»ÑÑÑÑÐµ ÐºÐ»ÐµÑÐ¾Ðº MDCK, ÑÐ¾Ð³Ð´Ð° ÐºÐ°Ðº Ñ Ð²Ð¸ÑÑÑÐ° H7N9 Ð½Ð°Ð±Ð»ÑÐ´Ð°Ð»Ð¾ÑÑ ÑÑÐ¸Ð»ÐµÐ½Ð¸Ðµ ÑÐ¾ÑÑÐ¾Ð²ÑÑ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸Ðº Ð¿ÑÐ¸ Ð·Ð°Ð¼ÐµÐ½Ðµ NP Ð³ÐµÐ½Ð° Ð½Ð° Ð±Ð¾Ð»ÐµÐµ ÑÐ¾Ð²ÑÐµÐ¼ÐµÐ½Ð½ÑÐ¹. ÐÐºÐ»ÑÑÐµÐ½Ð¸Ðµ ÑÐ¾Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð³Ð¾ NP Ð² Ð³ÐµÐ½Ð¾Ð¼ Ð´Ð¾Ð½Ð¾ÑÐ° Ð°ÑÑÐµÐ½ÑÐ°ÑÐ¸Ð¸ Ð´ÑÐ°Ð¼Ð°ÑÐ¸ÑÐµÑÐºÐ¸ Ð²Ð»Ð¸ÑÐ»Ð¾ Ð½Ð° ÑÐµÐ¿ÑÐ¾Ð´ÑÐºÑÐ¸Ñ Ð²Ð¸ÑÑÑÐ° Ð² Ð ÐÐ­ Ð¿ÑÐ¸ Ð¿Ð¾Ð½Ð¸Ð¶ÐµÐ½Ð½Ð¾Ð¹ ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÐµ Ð¸Ð½ÐºÑÐ±Ð°ÑÐ¸Ð¸, Ð¾Ð´Ð½Ð°ÐºÐ¾ ÑÑÐ¾Ñ ÑÑÑÐµÐºÑ Ð¿ÑÐ°ÐºÑÐ¸ÑÐµÑÐºÐ¸ Ð½Ð¸Ð²ÐµÐ»Ð¸ÑÐ¾Ð²Ð°Ð»ÑÑ Ð¿ÑÐ¸ Ð²ÐºÐ»ÑÑÐµÐ½Ð¸Ð¸ Ð² ÑÐµÐ°ÑÑÐ¾ÑÑÐ°Ð½ÑÐ½ÑÐ¹ ÑÑÐ°Ð¼Ð¼ Ð¿Ð¾Ð²ÐµÑÑÐ½Ð¾ÑÑÐ½ÑÑ Ð°Ð½ÑÐ¸Ð³ÐµÐ½Ð¾Ð² HA Ð¸ NA Ð¾Ñ ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²ÑÑÑÐ¸Ñ ÑÐ¿Ð¸Ð´ÐµÐ¼Ð¸ÑÐµÑÐºÐ¸Ñ Ð²Ð¸ÑÑÑÐ¾Ð². Ð¢Ð°ÐºÐ¶Ðµ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ ÑÐµÐ°ÑÑÐ¾ÑÑÐ°Ð½ÑÐ½ÑÐµ ÑÑÐ°Ð¼Ð¼Ñ ÐÐÐ Ñ ÑÐ¾ÑÐ¼ÑÐ»Ð¾Ð¹ Ð³ÐµÐ½Ð¾Ð¼Ð° 5:3 ÑÐ¾ÑÑÐ°Ð½ÑÑÑ ts/ca ÑÐµÐ½Ð¾ÑÐ¸Ð¿Ñ Ð² Ð ÐÐ­, Ð¿ÑÐ¸ÑÑÑÐ¸Ðµ ÐºÐ»Ð°ÑÑÐ¸ÑÐµÑÐºÐ¸Ð¼ ÑÐµÐ°ÑÑÐ¾ÑÑÐ°Ð½ÑÐ½ÑÐ¼ ÑÑÐ°Ð¼Ð¼Ð°Ð¼ Ñ ÑÐ¾ÑÐ¼ÑÐ»Ð¾Ð¹ Ð³ÐµÐ½Ð¾Ð¼Ð° 6:2.
description: Were studied the reproductive activity of reassortant influenza virus strains containing nucleoprotein (NP) from viruses isolated in different years. A side-by-side comparison of live attenuated influenza vaccine (LAIV) strains containing NP from old strain - master donor virus (genome composition 6:2) and currently circulating H1N1 or H7N9 viruses (genome composition 5:3) revealed multidirectional effect of NP gene on growth characteristics of LAIV. Infectious titers of 5:3 reassortant of H1N1 subtype were significantly lower in eggs and MDCK cells, compared to 6:2 reassortant. In contrast, H7N9 reassortant virus became more infectious when the old NP gene was replaced with the corresponding gene of wild-type influenza virus. The inclusion of wild-type NP in genome of the master donor virus dramatically affected the reproduction of the virus at low temperature in CE. However, this effect almost disappeared when corresponding wild-type surface antigens, HA and NA, were added to the reassortant viruses. Importantly, the LAIV reassortant strains with 5:3 genome composition had the same ts/ca phenotypes as the classical reassortant strains with 6:2 genome composition.