title: Ð¡ÑÐ°Ð²Ð½Ð¸ÑÐµÐ»ÑÐ½Ð°Ñ Ñ Ð°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ° Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð½ÑÐºÐ»ÐµÐ¸Ð½Ð¾Ð²ÑÑ ÐºÐ¸ÑÐ»Ð¾Ñ Ð¸Ð· Ð¾Ð±ÑÐ°Ð·ÑÐ¾Ð² Ð·ÑÐ±Ð½Ð¾Ð³Ð¾ Ð½Ð°Ð»ÐµÑÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Comparison of nucleic acid extraction methods from dental plaque samples
Creators: name: ÐÐ±ÑÐ°Ð·ÑÐ¾Ð²Ð°, ÐÐ»ÑÐ³Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Ð½ÑÐºÐ»ÐµÐ¸Ð½Ð¾Ð²ÑÐµ ÐºÐ¸ÑÐ»Ð¾ÑÑ
Ð¼ÐµÑÐ¾Ð´Ñ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ
Ð·ÑÐ±Ð½Ð¾Ð¹ Ð½Ð°Ð»ÐµÑ
ÐÐ¦Ð -Ð Ð
nucleic acid
extraction methods
dental plaque
qPCR

description: Ð¦ÐµÐ»Ñ ÑÐ°Ð±Ð¾ÑÑ â ÑÑÐ°Ð²Ð½Ð¸ÑÑ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÐµ Ð¼ÐµÑÐ¾Ð´Ñ Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð½ÑÐºÐ»ÐµÐ¸Ð½Ð¾Ð²ÑÑ ÐºÐ¸ÑÐ»Ð¾Ñ Ð¸Ð· Ð¾Ð±ÑÐ°Ð·ÑÐ¾Ð² Ð·ÑÐ±Ð½Ð¾Ð³Ð¾ Ð½Ð°Ð»ÐµÑÐ° ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°.Â  ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: 1. ÐÐ·ÑÑÐµÐ½Ð¸Ðµ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¿Ð¾ Ð¼Ð¸ÐºÑÐ¾Ð±Ð¸Ð¾ÑÐµ ÑÐ¾ÑÐ¾Ð²Ð¾Ð¹ Ð¿Ð¾Ð»Ð¾ÑÑÐ¸. 2. ÐÑÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð½ÑÐºÐ»ÐµÐ¸Ð½Ð¾Ð²ÑÑ ÐºÐ¸ÑÐ»Ð¾Ñ Ð¸Ð· Ð¾Ð±ÑÐ°Ð·ÑÐ¾Ð² Ð·ÑÐ±Ð½Ð¾Ð³Ð¾ Ð½Ð°Ð»ÐµÑÐ° Ð¼ÐµÑÐ¾Ð´Ð°Ð¼Ð¸ ÑÐ¿Ð¸ÑÑÐ¾Ð²Ð¾Ð³Ð¾ Ð¾ÑÐ°Ð¶Ð´ÐµÐ½Ð¸Ñ, ÑÐ¾ÑÐ±ÑÐ¸Ð¸ Ð½Ð° ÑÐ°ÑÑÐ¸ÑÐ°Ñ ÑÐ¸Ð»Ð¸ÐºÐ°Ð³ÐµÐ»Ñ, ÑÐ¾ÑÐ±ÑÐ¸Ð¸ Ð½Ð° Ð¼Ð°Ð³Ð½Ð¸ÑÐ½ÑÑ ÑÐ°ÑÑÐ¸ÑÐ°Ñ Ð¸ ÑÐµÑÐ¼Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð»Ð¸Ð·Ð¸ÑÐ°. 3. ÐÐ¾ÑÑÐ°Ð½Ð¾Ð²ÐºÐ° ÐÐ¦Ð  Ð² ÑÐµÐ¶Ð¸Ð¼Ðµ ÑÐµÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð²ÑÐµÐ¼ÐµÐ½Ð¸ Ñ Ð¿ÑÐ°Ð¹Ð¼ÐµÑÐ°Ð¼Ð¸ Ðº Ð³ÐµÐ½Ñ MGL. 4. ÐÐ½Ð°Ð»Ð¸Ð· Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð´Ð°Ð½Ð½ÑÑ. ÐÐ±ÑÐµÐºÑÐ°Ð¼Ð¸ Ð´Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð²ÑÐ±ÑÐ°Ð½Ñ Ð½Ð°Ð±Ð¾ÑÑ, Ð¿ÑÐ¸Ð¼ÐµÐ½ÑÐµÐ¼ÑÐµ Ð´Ð»Ñ ÑÐ°Ð·-Ð»Ð¸ÑÐ½ÑÑ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð½ÑÐºÐ»ÐµÐ¸Ð½Ð¾Ð²ÑÑ ÐºÐ¸ÑÐ»Ð¾Ñ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´Ð¸ÑÐµÐ»Ñ ÐÐ Â«ÐÐ»ÐºÐ¾Ñ ÐÐ¸Ð¾Â». ÐÑÐµÐ´Ð¼ÐµÑÐ¾Ð¼ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐ²Ð»ÑÐµÑÑÑ ÐºÐ°ÑÐµÑÑÐ²ÐµÐ½Ð½Ð°Ñ Ð¸ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²ÐµÐ½Ð½Ð°Ñ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ° Ð½ÑÐºÐ»ÐµÐ¸Ð½Ð¾Ð²ÑÑ ÐºÐ¸ÑÐ»Ð¾Ñ, Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½ÑÑ ÑÐ°Ð·Ð½ÑÐ¼Ð¸ Ð¼ÐµÑÐ¾Ð´Ð°Ð¼Ð¸. ÐÑÐ¸ Ð½Ð°Ð¿Ð¸ÑÐ°Ð½Ð¸Ð¸ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±Ð·Ð¾ÑÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ: eLIBRARY.RU. Scopus, NCBI.Â  Ð ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° ÑÐºÑÑÑÐ°ÐºÑÐ¸Ñ Ð½ÑÐºÐ»ÐµÐ¸Ð½Ð¾Ð²ÑÑ ÐºÐ¸ÑÐ»Ð¾Ñ Ð¸Ð· Ð¾Ð±ÑÐ°Ð·ÑÐ¾Ð² Ð·ÑÐ±Ð½Ð¾Ð³Ð¾ Ð½Ð°Ð»ÐµÑÐ° ÑÐ»ÐµÐ´ÑÑÑÐ¸Ð¼Ð¸ Ð¼ÐµÑÐ¾Ð´Ð°Ð¼Ð¸: ÑÐ¿Ð¸ÑÑÐ¾Ð²Ð¾Ðµ Ð¾ÑÐ°Ð¶Ð´ÐµÐ½Ð¸Ðµ, ÑÐµÑÐ¼Ð¸ÑÐµÑÐºÐ¸Ð¹ Ð»Ð¸Ð·Ð¸Ñ, ÑÐ¾ÑÐ±ÑÐ¸Ñ Ð½Ð° ÑÐ°ÑÑÐ¸ÑÐ°Ñ ÑÐ¸Ð»Ð¸ÐºÐ°Ð³ÐµÐ»Ñ, ÑÐ¾ÑÐ±ÑÐ¸Ñ Ð½Ð° Ð¼Ð°Ð³Ð½Ð¸ÑÐ½ÑÑ ÑÐ°ÑÑÐ¸ÑÐ°Ñ. ÐÐ»Ñ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð² ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸ Ð¿Ð¾ÑÑÐ°Ð²Ð»ÐµÐ½Ð° ÐÐ¦Ð  Ð² ÑÐµÐ°Ð»ÑÐ½Ð¾Ð¼ Ð²ÑÐµÐ¼ÐµÐ½Ð¸ Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ñ ÐºÑÐ¸Ð²ÑÐµ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸. Ð¡ÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÑÑ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»Ð¸ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿Ð°ÐºÐµÑÐ° Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼ Microsoft Excel. ÐÐ° Ð¾ÑÐ½Ð¾Ð²Ðµ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð²ÑÑÑÐ½ÐµÐ½Ð¾, ÑÑÐ¾ Ð´Ð»Ñ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð½ÑÐºÐ»ÐµÐ¸Ð½Ð¾Ð²ÑÑ ÐºÐ¸ÑÐ»Ð¾Ñ Ð¸Ð· Ð¾Ð±ÑÐ°Ð·ÑÐ¾Ð² Ð·ÑÐ±Ð½Ð¾Ð³Ð¾ Ð½Ð°Ð»ÐµÑÐ° Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ Ð¿Ð¾Ð´ÑÐ¾Ð´ÑÑÐ¸Ð¼ ÑÐ²Ð»ÑÐµÑÑÑ Ð½Ð°Ð±Ð¾Ñ Â«ÐÐ°Ð³Ð½Ð¾-ÐÐ-ÐÐ¸Ð¾Â».
description: The purpose of this work is to compare different methods nucleic acid extraction from human plaque samples. The tasks of this study were as follows: 1. Study of literature on the microbiota of the oral cavity. 2. Nucleic acid extraction from plaque samples by ethanol precipitation, adsorp-tion on silica gel particles, adsorption on magnetic particles and thermal lysis. 3. Conduction of real-time PCR using primers for the MGL gene. 4. Analysis of the received data. The objects of this study were kits used for various methods of nucleic acid ex-traction made by Alkor Bio Group. The subject of the study is the qualitative and quantitative characteristics of nucleic acids isolated by different methods. When writing the literature review, the following databases were used: eLI-BRARY.RU, Scopus, NCBI. During the study, nucleic acid was extracted from plaque samples by the following methods: ethanol precipitation, thermal lysis, adsorption on silica gel particles, and adsorption on magnetic particles. To analyze the extraction results, real-time PCR was performed and fluorescence curves were obtained. Statistical processing of experimental data was carried out using the Microsoft Excel software package. Based on the obtained data, it was found that the Â«Magno-NK-BioÂ» kit is the most suitable for nucleic acid analysis in dental plaque samples.