Additional file 1 of Chickpea shows genotype-specific nodulation responses across soil nitrogen environment and root disease resistance categories
Creators: name: Plett, Krista L.
name: Bithell, Sean L.
name: Dando, Adrian
name: Plett, Jonathan M.
Publisher: name: figshare
Year: 2021
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: Plant Biology

description: Additional file 1: Supplemental Figure SM1. Number of genes significantly differentially expressed in chickpea roots with differing nodulation after inoculation with Mesorhizobium ciceri. Number of genes significantly up- (blue) or down- (orange) regulated (log2FC &gt;1, p &lt; 0.05) in the roots of six chickpea genotypes three days after inoculation with M. ciceri as compared to sterile controls. Average number of nodules formed by plants at harvest are indicated (grey line). Supplemental Table SM1. Correlation coefficient results for the six genotype experiment with soil nitrogen treatments for measurements after four weeks of plant height, leaf and branch number, number of nodules, number of nodules per g of root, root dry weight (DW), root to shoot DW ratio, shoot DW and total plant DW. *indicates P &lt; 0.05 for a two-sided test of correlations different from zero. Supplemental Table SM2. Varietal comparison results for the controlled environment study of 29 chickpea genotypes with a range of root disease resistance ratings, for the variates: number of nodules (no. nod.); root (g.), shoot (g.), total plant (tot. pl, g) dry weights (DW); root:shoot (R:S) DW ratio per plant; number of nodules/g of root (no. nod/g); and nodule ethylene production (eth ppm per g nod). Root disease resistance abbreviations Fusarium wilt (FW), Phytophthora root rot (PRR), resistant (R), asymptomatic (AS), moderately resistant (MR), Dry root rot (DRR), Botrytis grey mould (BGM), moderately susceptible (MS), susceptible (S). P values, standard error of differences (SED) and least significant difference (LSD) values from ANOVA are included. For the Indian (ICC lines) and Australian (all non-ICC lines; PRR) lines the ratio between the highest and lowest value (L:S ratio) for each variate is also presented. *Genotypes studied for transcriptomic responses. Supplemental Table SM3. Correlation coefficient results for the 29 genotype experiment for measurements of number of nodules, number of nodules per g of root, average weight of nodules, ethylene production per gram of nodule, root dry weight (DW) shoot DW, root:shoot DW ratio and total plant DW. *indicates P &lt; 0.05 for a two-sided test of correlations different from zero. Supplemental Table SM4. List of chickpea genes contributing to the separation of high and low nodulating chickpea genotypes in PLS-DA analysis (based on loadings coordinates with r &gt; 0.013) of gene expression 3 days after inoculation with Mesorhizobium ciceri. Loadings coordinates on components 1 and 2 and radius are indicated, along with the gene and GO annotation. Supplemental Table SM5. List of chickpea genes and annotations in known pathways for early signalling and responses to rhizobia. Annotations were assigned based on either protein BLAST to known Medicago truncatula or Arabidopsis homologues if indicated, or gene assignments based on the Cicer arietinum (ICC4958) genome. * taken from Plett et al. 2016 [13] **genes identified based on Gifford et al. 2018 [16].