title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ Ð¼Ð¾Ð½Ð¾ÑÐ¸ÑÐ°ÑÐ½Ð¾-Ð¼Ð°ÐºÑÐ¾ÑÐ°Ð³Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÐºÐ¾Ð¼Ð¿Ð¾Ð½ÐµÐ½ÑÐ° Ð¸Ð¼Ð¼ÑÐ½Ð½Ð¾Ð³Ð¾ Ð¼Ð¸ÐºÑÐ¾Ð¾ÐºÑÑÐ¶ÐµÐ½Ð¸Ñ Ð¾Ð¿ÑÑ Ð¾Ð»Ð¸ Ð½Ð° ÑÐµÐ·Ð¸ÑÑÐµÐ½ÑÐ½Ð¾ÑÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð°Ð´ÐµÐ½Ð¾ÐºÐ°ÑÑÐ¸Ð½Ð¾Ð¼Ñ Ð»ÐµÐ³ÐºÐ¾Ð³Ð¾ Ðº ÑÑÐ¾Ð¿Ð¾Ð·Ð¸Ð´Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Impact of the monocyte-macrophage component of the immune tumor microenvironment on lung adenocarcinoma cells resistance to etoposide
Creators: name: ÐÐµÐ²Ð´Ð°ÑÐ°, ÐÐ°Ð»ÐµÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Ð±ÐµÐ»ÐºÐ¸ ÑÐµÐ¿Ð»Ð¾Ð²Ð¾Ð³Ð¾ ÑÐ¾ÐºÐ°
HSF1
Ð¾Ð¿ÑÑ Ð¾Ð»Ñ-Ð°ÑÑÐ¾ÑÐ¸Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐµ Ð¼Ð°ÐºÑÐ¾ÑÐ°Ð³Ð¸
ÑÐµÐ·Ð¸ÑÑÐµÐ½ÑÐ½Ð¾ÑÑÑ
ÐºÐ»ÐµÑÐºÐ¸ Ð°Ð´ÐµÐ½Ð¾ÐºÐ°ÑÑÐ¸Ð½Ð¾Ð¼Ñ Ð»ÐµÐ³ÐºÐ¾Ð³Ð¾
THP-1
ÑÑÐ¾Ð¿Ð¾Ð·Ð¸Ð´
heat-shock proteins
tumor-associated macrophages
resistance
lung adenocarcinoma cells
etoposide

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð¼Ð¾Ð½Ð¾ÑÐ¸Ñ-Ð¿Ð¾Ð´Ð¾Ð±Ð½Ð¾Ð¹ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ Ð»Ð¸Ð½Ð¸Ð¸ Ð¢ÐP-1 Ð½Ð° Ð¿ÑÐ¸Ð¾Ð±ÑÐµÑÐµÐ½Ð¸Ðµ ÑÐµÐ·Ð¸ÑÑÐµÐ½ÑÐ½Ð¾ÑÑÐ¸ ÐºÐ»ÐµÑÐ¾Ðº Ð°Ð´ÐµÐ½Ð¾ÐºÐ°ÑÑÐ¸Ð½Ð¾Ð¼Ñ Ð»ÐµÐ³ÐºÐ¾Ð³Ð¾ Ð549 Ð¿ÑÐ¸ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÐµ ÑÑÐ¾Ð¿Ð¾Ð·Ð¸Ð´Ð¾Ð¼. ÐÐ»Ñ Ð´Ð¾ÑÑÐ¸Ð¶ÐµÐ½Ð¸Ñ Ð¿Ð¾ÑÑÐ°Ð²Ð»ÐµÐ½Ð½Ð¾Ð¹ ÑÐµÐ»Ð¸ Ð±ÑÐ»Ð¸ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ñ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð¼ÐµÑÐ¾Ð´Ñ: ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð»Ð¸Ð½Ð¸Ð¹, Ð²ÐµÑÑÐµÑÐ½-Ð±Ð»Ð¾ÑÑÐ¸Ð½Ð³, Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð¶Ð¸Ð·Ð½ÐµÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÐ¸ ÐºÐ»ÐµÑÐ¾Ðº Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÑÐµÑÑÐ° Ð½Ð° Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð»Ð°ÐºÑÐ°ÑÐ´ÐµÐ³Ð¸Ð´ÑÐ¾Ð³ÐµÐ½Ð°Ð·Ñ Ð¸ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²ÐµÐ½Ð½Ð¾Ð¹ Ð¾ÑÐµÐ½ÐºÐ¸ ÐÐ¢Ð¤, ÑÐ¸ÑÑÐµÐ¼Ð° xCELLigence, ÑÐ±Ð¾ÑÐºÐ° ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ ÑÑÐµÑÐ¾Ð¸Ð´Ð¾Ð². Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð¼Ð¾Ð½Ð¾ÑÐ¸Ñ- Ð¿Ð¾Ð´Ð¾Ð±Ð½Ð°Ñ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð°Ñ Ð»Ð¸Ð½Ð¸Ñ THP-1 ÑÑÐ¸Ð¼ÑÐ»Ð¸ÑÑÐµÑ Ð°ÐºÑÐ¸Ð²Ð°ÑÐ¸Ñ HSF1 Ð² ÐºÐ»ÐµÑÐºÐ°Ñ Ð°Ð´ÐµÐ½Ð¾ÐºÐ°ÑÑÐ¸Ð½Ð¾Ð¼Ñ Ð»ÐµÐ³ÐºÐ¾Ð³Ð¾ A549, ÑÐµÐ¼ ÑÐ°Ð¼ÑÐ¼ Ð¿Ð¾Ð²ÑÑÐ°Ñ ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾ÑÑÑ Ð¿Ð¾ÑÐ»ÐµÐ´Ð½Ð¸Ñ Ðº Ð¿ÑÐ¾ÑÐ¸Ð²Ð¾Ð¾Ð¿ÑÑÐ¾Ð»ÐµÐ²Ð¾Ð¼Ñ Ð°Ð³ÐµÐ½ÑÑ ÑÑÐ¾Ð¿Ð¾Ð·Ð¸Ð´Ñ. Ð ÑÐ²ÑÐ·Ð¸ Ñ ÑÐµÐ¼, ÑÑÐ¾ Ð²Ð¾Ð¿ÑÐ¾Ñ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð¼Ð¾Ð½Ð¾ÑÐ¸ÑÐ°ÑÐ½Ð¾-Ð¼Ð°ÐºÑÐ¾ÑÐ°Ð³Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÐºÐ¾Ð¼Ð¿Ð¾Ð½ÐµÐ½ÑÐ° Ð½Ð° HSF1 Ð² Ð¾Ð¿ÑÑÐ¾Ð»ÐµÐ²ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ Ð´Ð¾ ÑÐ¸Ñ Ð¿Ð¾Ñ Ð½Ðµ ÑÐ°ÑÑÐ¼Ð°ÑÑÐ¸Ð²Ð°Ð»ÑÑ Ð² Ð½Ð°ÑÑÐ½ÑÑ ÑÐ°Ð±Ð¾ÑÐ°Ñ, Ð´Ð°Ð½Ð½Ð¾Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÐµÑ ÑÐ°ÑÑÐ¸ÑÐ¸ÑÑ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÐµÐ½Ð¸Ñ Ð¾ ÑÐ¿Ð¾ÑÐ¾Ð±Ð°Ñ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ð¹ Ð¼ÐµÐ¶Ð´Ñ Ð´Ð²ÑÐ¼Ñ Ð²ÑÑÐµÑÐºÐ°Ð·Ð°Ð½Ð½ÑÐ¼Ð¸ ÑÑÐ°ÑÑÐ½Ð¸ÐºÐ°Ð¼Ð¸ Ð¾Ð¿ÑÑÐ¾Ð»ÐµÐ²Ð¾Ð³Ð¾ Ð¼Ð¸ÐºÑÐ¾Ð¾ÐºÑÑÐ¶ÐµÐ½Ð¸Ñ. ÐÑÐ»ÐµÐ´ÑÑÐ²Ð¸Ðµ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÐ¸ Ð¼Ð¾Ð½Ð¾ÑÐ¸ÑÐ¾Ð² ÑÐ²ÐµÐ»Ð¸ÑÐ¸Ð²Ð°ÑÑ ÑÐµÐ·Ð¸ÑÑÐµÐ½ÑÐ½Ð¾ÑÑÑ Ð¾Ð¿ÑÑÐ¾Ð»ÐµÐ¹ Ðº ÑÐµÑÐ°Ð¿Ð¸Ð¸, Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð¾Ð² Ð´Ð°Ð½Ð½Ð¾Ð³Ð¾ ÑÐ²Ð»ÐµÐ½Ð¸Ñ ÑÐ²Ð»ÑÐµÑÑÑ Ð¿ÐµÑÑÐ¿ÐµÐºÑÐ¸Ð²Ð½Ð¾Ð¹ Ð·Ð°Ð´Ð°ÑÐµÐ¹, ÑÐµÑÐµÐ½Ð¸Ðµ ÐºÐ¾ÑÐ¾ÑÐ¾Ð¹ Ð¿Ð¾Ð·Ð²Ð¾Ð»Ð¸Ñ Ð±Ð¾Ð»ÐµÐµ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ Ð±Ð¾ÑÐ¾ÑÑÑÑ Ñ ÑÐ°Ð·Ð²Ð¸ÑÐ¸ÐµÐ¼ Ð·Ð»Ð¾ÐºÐ°ÑÐµÑÑÐ²ÐµÐ½Ð½ÑÑ Ð½Ð¾Ð²Ð¾Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ð¹.
description: The given work is devoted to study the effects of monocytic cell line THP-1 on the acquisition of resistance in A549 lung adenocarcinoma cells when treated with etoposide. To achieve the aim the following methods were applied: cultivation of cell lines, western-blotting, LDH cell viability essay and cell viability assay based on quantitation of ATP present, xCELLigence, cell spheroids formation. This study have shown that monocytic cell line THP-1 stimulates HSF1 activation in A549 lung adenocarcinoma cells, thereby increasing the resistance to the anti-tumor agent etoposide. Due to the fact that the influence of the monocyte-macrophage component on HSF1 in tumor cells has not been considered in scientific works so far, this study allows to expand understanding of the ways underlying the interaction between the two above-mentioned participants in the tumor microenvironment. Due to the ability of monocytes to increase tumor resistance to therapy, studying the mechanisms of this phenomenon is a promising task, the solution of which would allow to fight the development of malignant neoplasms more effective.