title: Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° ÑÐµÑÑ-ÑÐ¸ÑÑÐµÐ¼Ñ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ Ð¸Ð¼Ð¼ÑÐ½Ð¾ÑÐµÑÐ¼ÐµÐ½ÑÐ½Ð¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð´Ð»Ñ ÐºÐ¾Ð½ÑÑÐ¾Ð»Ñ ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ñ ÐºÐ°Ð·ÐµÐ¸Ð½Ð° ÐºÐ¾ÑÐ¾Ð²ÑÐµÐ³Ð¾ Ð¼Ð¾Ð»Ð¾ÐºÐ° Ð² Ð¿Ð¸ÑÐµÐ²Ð¾Ð¹ Ð¿ÑÐ¾Ð´ÑÐºÑÐ¸Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Development of a test system based on enzyme immunoassay to control the content of cows milk casein in food products
Creators: name: ÐÐ¼Ð¸ÑÑÐ¸ÐµÐ²Ð°, ÐÐ½ÑÐ¸ÑÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: ÐºÐ°Ð·ÐµÐ¸Ð½ ÐºÐ¾ÑÐ¾Ð²ÑÐµÐ³Ð¾ Ð¼Ð¾Ð»Ð¾ÐºÐ°
Ð°Ð»Ð»ÐµÑÐ³Ð¸Ñ
Ð¸Ð¼Ð¼ÑÐ½Ð¾ÑÐµÑÐ¼ÐµÐ½ÑÐ½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð· ÐÐ¤Ð
Ð¼Ð¾Ð½Ð¾ÐºÐ»Ð¾Ð½Ð°Ð»ÑÐ½ÑÐµ Ð°Ð½ÑÐ¸ÑÐµÐ»Ð°
Ð²Ð°Ð»Ð¸Ð´Ð°ÑÐ¸Ñ ÑÐµÑÑ-ÑÐ¸ÑÑÐµÐ¼Ñ
cows milk casein
elisa immunoassay
allergy
monoclonal antibodies
test system validation

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description: The work describes approaches to the development of a test system based on enzyme immunoassay, stages of production of monoclonal antibodies to casein, testing them for sensitivity and cross-reactivity with milk proteins of other animal species (horse, camel, goat). During the study, an antibody was selected, which was taken as the basis of the test system, and a conjugate was selected, which constitutes a pair of antibody - HRP antibody. It has been proven that this pair (antibody - conjugate) most accurately allows identifying the concentration of casein in the studied sample of the dairy product. The purpose of our study was to create an enzyme immunoassay test system for quality control of raw materials and finished food products without the declared content of cows milk casein. The conducted studies are aimed at identifying the operability of the test system based on the X3 antibody, quantitative indicators of the detection limit, the limit of quantification, the linearity of the system and the percentage of sample extraction were determined. Instructions for working with this test system for the purpose of quantitatively determining the casein content in the sample under study were developed.