title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸Ñ ÑÐµÑÐµÐ±ÑÐ° Ð½Ð° Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð¼ÐµÐ´Ñ-ÑÐ²ÑÐ·ÑÐ²Ð°ÑÑÐ¸Ñ Ð±ÐµÐ»ÐºÐ¾Ð²
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Effect of silver nanoparticles on the activity of copper-binding proteins
Creators: name: ÐÑÐ¸ÑÑÐº, ÐÐ²Ð°Ð½
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2019
Subjects: Ð¼ÐµÐ´Ñ
Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¼ Ð¼ÐµÐ´Ð¸
ÑÐµÑÐµÐ±ÑÐ¾
Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸ÑÑ ÑÐµÑÐµÐ±ÑÐ°
ÑÐµÑÑÐ»Ð¾Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð½
copper
copper metabolism
silver
silver nanoparticles
ceruloplasmin

description: Ð¦ÐµÐ»Ñ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ â Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸Ñ ÑÐµÑÐµÐ±ÑÐ° (ÐÐ§Ð¡) ÑÐ°Ð·Ð½Ð¾Ð³Ð¾ ÑÐ°Ð·Ð¼ÐµÑÐ° Ñ Ð¼ÐµÐ´Ñ-ÑÐ²ÑÐ·ÑÐ²Ð°ÑÑÐ¸Ð¼Ð¸ Ð±ÐµÐ»ÐºÐ°Ð¼Ð¸ in vivo Ð¸ in vitro. ÐÐ§Ð¡ ÑÐ¸ÑÐ¾ÐºÐ¾ ÑÐ°ÑÐ¿ÑÐ¾ÑÑÑÐ°Ð½ÐµÐ½Ñ, Ð¸ Ð¿Ð¾Ð¿Ð°Ð´Ð°ÑÑ Ð² Ð±Ð¾Ð»ÑÑÐ¾Ð¼ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ðµ Ð² Ð¾ÐºÑÑÐ¶Ð°ÑÑÑÑ ÑÑÐµÐ´Ñ. ÐÑÐ¾Ð±Ð»ÐµÐ¼Ð° Ð±ÐµÐ·Ð¾Ð¿Ð°ÑÐ½Ð¾ÑÑÐ¸ ÐÐ§Ð¡ Ð´Ð»Ñ ÑÐºÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¸ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° ÑÐ²Ð»ÑÐµÑÑÑ Ð¸Ð½ÑÐµÑÐµÑÐ½Ð¾Ð¹, ÑÐ°Ðº ÐºÐ°Ðº Ð¾Ð½Ð¸ Ð¼Ð¾Ð³ÑÑ Ð²Ð»Ð¸ÑÑÑ Ð½Ð° Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¼ Ð¼ÐµÐ´Ð¸ Ð¼Ð»ÐµÐºÐ¾Ð¿Ð¸ÑÐ°ÑÑÐ¸Ñ. 
ÐÐ½ÑÑÑÐ¸Ð±ÑÑÑÐ¸Ð½Ð½Ð¾Ðµ Ð²Ð²ÐµÐ´ÐµÐ½Ð¸Ðµ ÐÐ§Ð¡ Ð¼ÑÑÐ°Ð¼ Ð² ÑÐµÑÐµÐ½Ð¸Ðµ Ð½ÐµÐ´ÐµÐ»Ð¸ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð»Ð¾ Ð¿Ð¾Ð½Ð¸Ð¶ÐµÐ½Ð¸Ðµ ÑÑÐ°ÑÑÑÐ° Ð¼ÐµÐ´Ð¸ Ð² ÑÑÐ²Ð¾ÑÐ¾ÑÐºÐµ ÐºÑÐ¾Ð²Ð¸. ÐÐµÐ¹ÑÑÐ²Ð¸Ðµ ÐÐ§Ð¡ Ð±Ð¾Ð»ÑÑÐµÐ³Ð¾ Ð´Ð¸Ð°Ð¼ÐµÑÑÐ° (Ð¾ÐºÐ¾Ð»Ð¾ 70 Ð½Ð¼) Ð±ÑÐ»Ð¾ Ð±Ð¾Ð»ÐµÐµ Ð²ÑÑÐ°Ð¶ÐµÐ½Ð¾, ÑÐµÐ¼ ÑÐ°ÑÑÐ¸Ñ Ð´Ð¸Ð°Ð¼ÐµÑÑÐ¾Ð¼ Ð¾ÐºÐ¾Ð»Ð¾ 10 Ð½Ð¼. ÐÑÐ¸ ÑÑÐ¾Ð¼ ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð½Ð¸Ðµ ÑÐµÑÑÐ»Ð¾Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð½Ð° (Ð¦Ð, (ÑÐµÑÑ-)Ð¾ÐºÑÐ¸Ð´Ð°Ð·Ð°, Ð¾ÑÐ½Ð¾Ð²Ð½Ð¾Ð¹ Ð¼ÐµÐ´Ñ-ÑÑÐ°Ð½ÑÐ¿Ð¾ÑÑÐ½ÑÐ¹ Ð±ÐµÐ»Ð¾Ðº ÑÑÐ²Ð¾ÑÐ¾ÑÐºÐ¸ ÐºÑÐ¾Ð²Ð¸) Ð¸ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ ÐµÐ³Ð¾ Ð³ÐµÐ½Ð° Ð½Ðµ Ð¼ÐµÐ½ÑÐ»Ð¸ÑÑ. ÐÐ¾Ð½Ñ ÑÐµÑÐµÐ±ÑÐ° Ð¸Ð· ÐÐ§Ð¡ ÑÐ²ÑÐ·ÑÐ²Ð°ÑÑÑÑ Ñ Ð¾Ð´Ð½Ð¸Ð¼ Ð¸Ð· Ð¼ÐµÐ´Ñ-ÑÐ²ÑÐ·ÑÐ²Ð°ÑÑÐ¸Ñ ÑÐ°Ð¹ÑÐ¾Ð² Ð¦Ð in vivo.
ÐÐµÐ»Ñ-ÑÐ¸Ð»ÑÑÑÐ°ÑÐ¸Ñ ÑÑÐ²Ð¾ÑÐ¾ÑÐ¾Ðº ÐºÑÐ¾Ð²Ð¸ Ð¼ÑÑÐµÐ¹ Ñ Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐ¸Ð¼ Ð¸Ð·Ð¼ÐµÑÐµÐ½Ð¸ÐµÐ¼ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¹ Ð¼ÐµÐ´Ð¸ Ð¸ ÑÐµÑÐµÐ±ÑÐ° Ð² ÑÑÐ¾Ð¼Ð°ÑÐ¾Ð³ÑÐ°ÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÑÐ°ÐºÑÐ¸ÑÑ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð»Ð¸, ÑÑÐ¾ Ð¿ÑÐ°ÐºÑÐ¸ÑÐµÑÐºÐ¸ Ð²ÑÑ Ð¼ÐµÐ´Ñ ÑÐ¾Ð´ÐµÑÐ¶Ð°Ð»Ð°ÑÑ Ð² ÑÐ¾ÑÑÐ°Ð²Ðµ ÐµÐ´Ð¸Ð½ÑÑÐ²ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¿Ð¸ÐºÐ° ÑÐ»ÑÑÐ¸Ð¸, ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²ÑÑÑÐµÐ³Ð¾ Ð¦Ð. Ð¡ÐµÑÐµÐ±ÑÐ¾ ÑÐ»ÑÐ¸ÑÐ¾Ð²Ð°Ð»Ð¾ÑÑ Ð² Ð´Ð²Ð° ÑÑÐ°Ð¿Ð°. ÐÐ´Ð¸Ð½ Ð¸Ð· Ð¿Ð¸ÐºÐ¾Ð² ÑÐ¾ÑÐ²ÐµÑÑÑÐ²Ð¾Ð²Ð°Ð» Ð¦Ð, Ð´ÑÑÐ³Ð¾Ð¹ ÑÐ»ÑÐ¸ÑÐ¾Ð²Ð°Ð»ÑÑ ÑÐ°Ð½ÑÑÐµ. 
Ð ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ñ in vitro Ð´Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ ÑÑÐµÑÐ¸ÑÐµÑÐºÐ¸Ðµ ÐÐ§Ð¡ Ð²ÑÐµÑ ÑÐ°Ð·Ð¼ÐµÑÐ¾Ð² Ð½Ðµ ÑÐ²ÑÐ·ÑÐ²Ð°ÑÑÑÑ Ñ Ð¦Ð Ð¸ Ð°Ð»ÑÐ±ÑÐ¼Ð¸Ð½Ð¾Ð¼. ÐÐ´Ð½Ð°ÐºÐ¾ ÐÐ§Ð¡ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²ÑÑÑ Ñ Ð±ÐµÐ»ÐºÐ¾Ð¼, ÑÐ»ÑÐ¸ÑÑÑÑÐ¸Ð¼ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð³ÐµÐ»Ñ-ÑÐ¸Ð»ÑÑÑÐ°ÑÐ¸Ð¸ ÑÐ°Ð½ÑÑÐµ, ÑÐµÐ¼ Ð¦Ð. ÐÐ¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾, ÑÐ°ÐºÐ¸Ð¼ Ð±ÐµÐ»ÐºÐ¾Ð¼ ÑÐ²Ð»ÑÐµÑÑÑ Ð°2-Ð¼Ð°ÐºÑÐ¾Ð³Ð»Ð¾Ð±ÑÐ»Ð¸Ð½.
description: The aim of this work is to study the interaction of silver nanoparticles (SNPs) of various sizes with copper-binding proteins both in vivo and in vitro. SNPs are widespread, and are currently released in large quantities into the environment. The problem of safety of SNPs for ecology and humans is an interesting one, since they can affect the copper metabolism of mammals.
Intraperitoneal administration of SNPs to mice during the week showed a decrease in serum copper status. The effect of SNPs of a larger diameter (about 70 nm) was more pronounced than of particles with a diameter of about 10 nm. At the same time, the content of ceruloplasmin (CP, (ferr-) oxidase, the main copper-transport protein in the blood serum) and the expression of its gene did not change. These data show that silver ions from nanoparticles bind to one of the copper-binding sites of CP in vivo.
Gel filtration of the blood serum of mice with further measurement of the concentration of copper and silver ions in the chromatographic fractions showed that almost all copper was contained in the composition of a single elution peak corresponding to the CP. Silver eluted in two steps. One of the peaks corresponded to the CP, the other eluted earlier.
In vitro experiments have shown that spherical SNPs of all sizes are not associated with ceruloplasmin and albumin. However, SNPs interact with protein, eluting during gel filtration earlier than CP. Perhaps this protein is a2-macroglobulin.