Additional file 1 of CDC50A might be a novel biomarker of epithelial ovarian cancer-initiating cells
Creators: name: Yin, Jie
name: Wen, Yiping
name: Zeng, Jing
name: Zhang, Yanyan
name: Chen, Jiayu
name: Zhang, Yanmei
name: Han, Tiantian
name: Li, Xiaoying
name: Huang, Hong
name: Cai, Yan
name: Jin, Ying
name: Li, Yan
name: Guo, Wei
name: Pan, Lingya
Publisher: name: figshare
Year: 2022
Subjects: Biochemistry
Cell Biology
Chemical Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Developmental Biology
Cancer
Hematology

description: Additional file 1: Supplementary Figure 1. Screening of shRNAs targeting CDC50A. Supplementary Figure 2. Validation of CDC50A expression and location after transfection with pLVX-CDC50A-GFP viruses in SKOV3. Sphere forming test in SKOV3 with up-regulated CDC50A. Supplementary Figure 3. All mice and xenografts pictures. Supplementary Figure 4. Xenograft tumor tissues (PID003) was confirmed by immunohistochemistry. Supplementary Figure 5. The frequency of CDC50A + Lin- cells in clinical patients. Supplementary Table 1. List of gene-specific primer sequences. Supplementary Table 2. The positive ratio of CDC50A, CD44, CD117 and CD133 in five EOC cell lines. Supplementary Table 3. Patient clinical records and frequency of CDC50A+Lin− cells. Supplementary Figure 6. The raw data of Fig. 2, panel A. The region used in Fig. 2 was marked with red box. Chemiluminescence strip was exposed on Kodak film. Then the image was generated by camera. The blots were cut prior to hybridisation with antibodies. The edge of all images have been exhibited. Supplementary Figure 7. The raw data of Fig. 3, panel F. The region used in Fig. 3F was marked with red box. The left three samples in red box were CDC50A+, the right three were CDC50A-. Marker was sampled on both sides of all samples. Gel was cut before protein transfer membrane. Middle lines of the markers were the both sides of gel. The blots were cut prior to hybridisation with antibodies. The images were generated by chemiluminescence image analysis system (Tanon 520, Shanghai, China). The right was the chemiluminescence exposure image. The left was the merge of daylight image with edge of blots and chemiluminescence exposure image. Supplementary Figure 8. The raw data of Fig. 3, panel F. The region used in Fig. 3F was marked with red box. The left three samples in red box were CDC50A+, the right three were CDC50A-. Marker was sampled on both sides of all samples. Gel was cut before protein transfer membrane. Middle lines of the markers were the both sides of gel. The blots were cut prior to hybridisation with antibodies. The images were generated by chemiluminescence image analysis system (Tanon 520, Shanghai, China). The right was the chemiluminescence exposure image. The left was the merge of daylight image with edge of blots and chemiluminescence exposure image. Supplementary Figure 9. The raw data of Fig. 4, panel D. The region used in Fig. 3F was marked with red box. The left band of Bmi-1, β-catenin, APC, Oct-4, Sox-2, Nanog, E-Cadherin, Vimentin, Notch-1, Lin28 and β-actin were CDC50A+, and the right was CDC50A-. The left band of TGF-β1 was CDC50A- and the right was CDC50A+. Supplementary Figure 10. The raw data of Supplementary Fig. 1. The image is generated by the chemiluminescence imaging system (Tanon 520, Shanghai, China). The blots were cut prior to hybridisation with antibodies. The whole raw images have been exhibited. The region used in Supplementary Fig. 1 was marked with red box. The edges of the blots (β-actin) in chemiluminescence exposure image were outlined with solid black lines. Supplementary Figure 11. The raw data of Supplementary Fig. 2, panel B. Chemiluminescence strip was exposed on Kodak film. The image was generated by camera. The blots were cut prior to hybridisation with antibodies. The edge of all images have been exhibited. The region used in Supplementary Fig. 2B was marked with red box.