title: Ð¥Ð°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ° Ð½Ð¾Ð²Ð¾Ð³Ð¾ Ð°Ð½ÑÐ¸Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÐ¸Ð°Ð·Ð¾Ð»-Ð¾ÐºÑÐ°Ð·Ð¾Ð»-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð° Ð¸Ð· K. pneumoniae
title: Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÑÐºÐ°Ñ ÑÐ°Ð±Ð¾ÑÐ°
titleType: Subtitle
Creators: name: Ð£ÑÐºÐ¸Ð½Ð°, ÐÑÐ¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2016
Subjects: ÑÐ¸Ð°Ð·Ð¾Ð»-Ð¾ÐºÑÐ°Ð·Ð¾Ð»-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐµ Ð¼Ð¸ÐºÑÐ¾ÑÐ¸Ð½Ñ
Ð¿ÐµÐ¿ÑÐ¸Ð´Ñ
Ð°Ð½ÑÐ¸Ð±Ð¸Ð¾ÑÐ¸ÐºÐ¸
Ð±Ð°ÐºÑÐµÑÐ¸Ð¸
Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð¾Ðµ ÐºÐ»Ð¾Ð½Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
thiazole-oxazole modified microcins
peptides
antibiotics
bacteria
molecular cloning

description: Ð¦ÐµÐ»ÑÑ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÐ»Ð°ÑÑ Ð¸Ð´ÐµÐ½ÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ñ Ð¸ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ° Ð½Ð¾Ð²Ð¾Ð³Ð¾ ÑÐ¸Ð°Ð·Ð¾Ð»-Ð¾ÐºÑÐ°Ð·Ð¾Ð»-Ð¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð° Ð¸Ð· Ð±Ð°ÐºÑÐµÑÐ¸Ð¸ Klebsiella pneumoniae subsp. ozaenae, Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð² ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð±Ð¸Ð¾Ð¸Ð½ÑÐ¾ÑÐ¼Ð°ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¿Ð¾Ð¸ÑÐºÐ° Ð½ÐµÐ¾ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸Ð·Ð¾Ð²Ð°Ð½Ð½ÑÑ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ñ ÐºÐ»Ð°ÑÑÐµÑÐ¾Ð², Ð³Ð¾Ð¼Ð¾Ð»Ð¾Ð³Ð¸ÑÐ½ÑÑ ÐºÐ»Ð°ÑÑÐµÑÑ Ð¼Ð¸ÐºÑÐ¾ÑÐ¸Ð½Ð° Ð17. ÐÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð¿ÑÐ¸ Ð³ÐµÑÐµÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð¹ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð¾Ð¿ÐµÑÐ¾Ð½Ð° Ð¸Ð· K. pneumoniae subsp. ozaenae Ð² ÐºÐ»ÐµÑÐºÐ°Ñ E. coli Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´Ð¸ÑÑÑ Ð²ÐµÑÐµÑÑÐ²Ð¾, ÑÑÑÑÐºÑÑÑÐ° ÐºÐ¾ÑÐ¾ÑÐ¾Ð³Ð¾ Ð±ÑÐ»Ð° ÑÐ°ÑÑÐ¸ÑÐ½Ð¾ Ð¾ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸Ð·Ð¾Ð²Ð°Ð½Ð° Ð¼ÐµÑÐ¾Ð´Ð°Ð¼Ð¸ Ð¼Ð°ÑÑ-ÑÐ¿ÐµÐºÑÑÐ¾Ð¼ÐµÑÑÐ¸Ð¸, Ð¸ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð²ÐµÑÐµÑÑÐ²Ð¾ ÑÐ¾Ð´ÐµÑÐ¶Ð¸Ñ ÑÐµÑÑÑÐµ Ð³ÐµÑÐµÑÐ¾ÑÐ¸ÐºÐ»Ð°: Ð¾Ð´Ð¸Ð½ Ð¾ÐºÑÐ°Ð·Ð¾Ð»ÑÐ½ÑÐ¹ Ð¸ ÑÑÐ¸ ÑÐ¸Ð°Ð·Ð¾Ð»ÑÐ½ÑÑ, - Ð° ÑÐ°ÐºÐ¶Ðµ Ð²ÐºÐ»ÑÑÐ°ÐµÑ Ð´Ð¾Ð¿Ð¾Ð»Ð½Ð¸ÑÐµÐ»ÑÐ½ÑÑ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ñ Ð½Ð° N-ÐºÐ¾Ð½ÑÐµ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð°. ÐÑÐ»Ð¾ Ð¿ÑÐ¾Ð´ÐµÐ¼Ð¾Ð½ÑÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¾, ÑÑÐ¾ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´Ð¸Ð¼Ð¾Ðµ ÑÐ¾ÐµÐ´Ð¸Ð½ÐµÐ½Ð¸Ðµ Ð¾Ð±Ð»Ð°Ð´Ð°ÐµÑ Ð°Ð½ÑÐ¸Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑÑ Ð² Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ð¸ E. coli, Ð¸ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð·Ð°Ð²Ð¸ÑÐ¸Ñ Ð¾Ñ Ð¿ÑÐ¸ÑÑÑÑÑÐ²Ð¸Ñ Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Ð½Ð° N-ÐºÐ¾Ð½ÑÐµ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð° Ð¸ Ð½ÐµÐ¼Ð¾Ð´Ð¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ Ð¡-ÐºÐ¾Ð½ÑÐµÐ²ÑÑ Ð°Ð¼Ð¸Ð½Ð¾ÐºÐ¸ÑÐ»Ð¾Ñ.
description: The aim of this work was to identify and describe the structure of a new thiazole-oxazole modified peptide from Klebsiella pneumoniae subsp. ozaenae. Cluster of genes of a new TOMM was found as a result of bioinformatic search of microcin B17 homologs. Detected cluster was introduced into a heterologous expression host, E. coli, which was shown to produce a substance belonging to the class of TOMM. The structure of produced peptide was partially characterized: mass spectrometric analysis revealed that it contains four heterocycles (one oxazole and three thiazole) and also includes an additional modification at the N-terminus of the peptide. We demonstrated that identified cluster of genes is capable of producing a biologically active molecule. Moreover, produced compound was shown to have an antibacterial activity against E. coli. As it was determined this activity depends on the presence of modification at the N-terminus of the peptide and unmodified C-terminal amino acids.