title: Ð¡Ð±Ð¾ÑÐºÐ° Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ð¹ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ð¸ Ð´Ð»Ñ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ¸ Ð¿ÑÐ¾Ð±Ð¸Ð¾ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÑÐ°Ð¼Ð¼Ð°-Ð¿ÑÐ¾Ð´ÑÑÐµÐ½ÑÐ° Ð°Ð¼ÐµÐ»Ð¾Ð³ÐµÐ½Ð¸Ð½Ð°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Assembling of a genetic construction for the development of a probiotic strain-producer of amelogenin
Creators: name: Ð¡ÐµÑÐ¾Ð², ÐÐ¾Ð½ÑÑÐ°Ð½ÑÐ¸Ð½
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: AMELX
Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð½Ð°Ñ ÑÐµÐ¿Ð½Ð°Ñ ÑÐµÐ°ÐºÑÐ¸Ñ
Ð°ÑÑÐ¸Ð¼ÐµÑÑÐ¸ÑÐ½Ð°Ñ ÐÐ¦Ð
Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ°Ñ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ñ
ÑÐ±Ð¾ÑÐ¾ÐºÐ° Ð³ÐµÐ½Ð° de novo
ÑÑÐ°Ð¼Ð¼Ñ E.coli
ÑÑÐ°Ð½ÑÑÐ¾ÑÐ¼Ð°ÑÐ¸Ñ
ÐÐ¦Ð -ÑÐºÑÐ¸Ð½Ð¸Ð½Ð³
polymerase chain reaction
asymmetric PCR
genetic construction
de novo gene assembly
E.coli
transformation
PCR-screening.

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«Ð¡Ð±Ð¾ÑÐºÐ° Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ð¹ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ð¸ Ð´Ð»Ñ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ¸ Ð¿ÑÐ¾Ð±Ð¸Ð¾ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÑÐ°Ð¼Ð¼Ð°-Ð¿ÑÐ¾Ð´ÑÑÐµÐ½ÑÐ° Ð°Ð¼ÐµÐ»Ð¾Ð³ÐµÐ½Ð¸Ð½Ð°Â».Â ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð´Ð¸Ð·Ð°Ð¹Ð½Ñ Ð¸ ÑÐ±Ð¾ÑÐºÐµ Ð³ÐµÐ½Ð°Â AMELX de novo.Â Ð¦ÐµÐ»Ñ ÑÐ°Ð±Ð¾ÑÑ â ÑÐ¾Ð±ÑÐ°ÑÑ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÑ Ð³ÐµÐ½Ð°, ÐºÐ¾Ð´Ð¸ÑÑÑÑÐµÐ³Ð¾ ÑÐµÐ»Ð¾Ð²ÐµÑÐµÑÐºÐ¸Ð¹ Ð±ÐµÐ»Ð¾Ðº Ð°Ð¼ÐµÐ»Ð¾Ð³ÐµÐ½Ð¸Ð½. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð¸Ñ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¸ ÑÐµÑÐµÐ½Ñ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð·Ð°Ð´Ð°ÑÐ¸: 1. ÐÑÐ¿Ð¾Ð»Ð½ÐµÐ½ Ð´Ð¸Ð·Ð°Ð¹Ð½ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÐµÐ¹ 19 Ð¾Ð»Ð¸Ð³Ð¾Ð½ÑÐºÐ»ÐµÐ¾ÑÐ¸Ð´Ð¾Ð² Ñ Ð¾Ð±Ð»Ð°ÑÑÑÐ¼Ð¸ Ð¿ÐµÑÐµÐºÑÑÐ²Ð°Ð½Ð¸Ñ Ð¸ Ñ Ð¸Ñ ÑÐ¿Ð¾ÑÑÐ´Ð¾ÑÐµÐ½Ð½ÑÐ¼ ÑÐ°ÑÐ¿Ð¾Ð»Ð¾Ð¶ÐµÐ½Ð¸ÐµÐ¼ Ð² ÑÐ¾ÑÑÐ°Ð²Ðµ Ð¿Ð¾Ð»Ð½Ð¾Ð³Ð¾ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ°.Â 2. ÐÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° ÐÐ¦Ð  ÑÐ±Ð¾ÑÐºÐ° Ð¿Ð¾Ð»Ð½Ð¾Ð¹ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ð¸ Ð¸Ð· Ð¾Ð»Ð¸Ð³Ð¾Ð½ÑÐºÐ»ÐµÐ¾ÑÐ¸Ð´Ð¾Ð². 3. ÐÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð¾ Ð¸ÑÐ¿ÑÐ°Ð²Ð»ÐµÐ½Ð¸Ðµ Ð¼ÑÑÐ°ÑÐ¸Ð¹ Ð² ÑÐµÐ»ÐµÐ²Ð¾Ð¹ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾ÑÑÐ¸. Ð Ð°Ð±Ð¾ÑÐ° Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ Ð¼Ð¸ÐºÑÐ¾Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ð¸ Ð² Ð¼ÐµÐ¶Ð´ÑÐ½Ð°ÑÐ¾Ð´Ð½Ð¾Ð¼ Ð½Ð°ÑÑÐ½Ð¾Ð¼ ÑÐµÐ½ÑÑÐµ SCAMT (Solution Chemistry of Advanced Materials and Technologies / Ð Ð°ÑÑÐ²Ð¾ÑÐ½Ð¾Ð¹ ÑÐ¸Ð¼Ð¸Ð¸ Ð¿ÐµÑÐµÐ´Ð¾Ð²ÑÑ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ð¾Ð² Ð¸ ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¹). Ð ÑÐ¾Ð´Ðµ Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ñ ÑÐµÑÐ¸Ð¸ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ¾Ð² Ð¿Ð¾ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ Ð±ÐµÐ·Ð¾ÑÐ¸Ð±Ð¾ÑÐ½Ð¾Ð¹ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ð¹ ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ð¸.Â 
description: The subject of the graduated qualification work is:Â Â«Assembling of a genetic construct for the development of a probiotic strain-producer of amelogeninÂ».Â This work deals with the design and assembly of theÂ AMELXÂ geneÂ de novo.Â The goal of the work is to assemble an error-free genetic construct encoding the human amelogenin protein. As a result of this work, the following tasks were accomplished: 1.Â Sequence design of 19 oligonucleotides with overlapping regions and their ordering as a part of complete genetic construction was performed.Â 2.Â PCR assembly of the complete oligonucleotide construct was performed. 3.Â Correction of mutations in the target sequence was performed.The work was conducted based on the laboratory of microbiology in the international scientific center SCAMT (Solution Chemistry of Advanced Materials and Technologies). During the final qualification work a series of experiments on obtaining an error-free genetic construct were carried out.