Additional file 1 of Lignin intermediates lead to phenyl acid formation and microbial community shifts in meso- and thermophilic batch reactors
Creators: name: Prem, Eva Maria
name: Mutschlechner, Mira
name: Blaz Stres
name: Illmer, Paul
name: Wagner, Andreas Otto
Publisher: name: figshare
Year: 2021
Subjects: subject: Microbiology
subject: FOS: Biological sciences
subject: FOS: Biological sciences

description: Additional file 1: Table S1. pH and concentrations of propionate, i-butyrate, and butyrate of mesophilic control and lignin intermediate samples under low, medium, and high overload conditions on day 0, 7, 14, and 28. Table S2. Concentrations of PAA, PPA, and PBA (sum) of mesophilic control and lignin intermediate samples under low, medium, and high overload conditions on day 0, 7, 14, and 28. Table S3. Concentrations of benzoate, hydroxybenzoate, and hydroxy-PAA of mesophilic control and lignin intermediate samples under low, medium, and high overload conditions on day 0, 7, 14, and 28. Table S4. pH and concentrations of propionate, i-butyrate, and butyrate of thermophilic control and lignin intermediate samples under low, medium, and high overload conditions on day 0, 7, 14, and 28. Table S5. Concentrations of PAA, PPA, and PBA (sum) of thermophilic control and lignin intermediate samples under low, medium, and high overload conditions on day 0, 7, 14, and 28. Table S6. Concentrations of benzoate, hydroxybenzoate, and hydroxy-PAA of thermophilic control and lignin intermediate samples under low, medium, and high overload conditions on day 0, 7, 14, and 28. Figure S1a. Mean sequence proportions [%] of mesophilic genera of all control and gallic, syringic, and vanillic acid samples. Figure S1b. Mean sequence proportions [%] of mesophilic genera of all control and ferulic acid and of all control and coumaric acid samples. Figure S2a. Interactive visualisation of mesophilic taxa of the control and all lignin intermediate samples under low, medium, and high overload conditions on day 0 and on day 14. Figure S2b. Interactive visualisation of mesophilic taxa of the control and all lignin intermediate samples under low, medium, and high overload conditions on day 0 and on day 28. Figure S3. KEGG orthology counts of general pathways and of pathways relevant for AD of aromatic compounds of all mesophilic samples of day 28. Figure S4a. Mean sequence proportions [%] of thermophilic genera of all control and gallic, syringic, and vanillic acid samples. Figure S4b. Mean sequence proportions [%] of thermophilic genera of all control and ferulic acid and of all control and coumaric acid samples. Figure S5a. Interactive visualisation of thermophilic taxa of the control and of all lignin intermediates samples under low, medium, and high overload conditions on day 0 and on day 14. Figure S5b. Interactive visualisation of thermophilic taxa of the control and of all lignin intermediate samples under low, medium, and high overload conditions on day 0 and on day 28. Figure S6. KEGG orthology counts of general pathways and of pathways relevant for AD of aromatic compounds of all thermophilic samples on day 28. Figure S7. Shannon diversity index for meso- and thermophilic samples fed with gallic, syringic, vanillic, ferulic, and coumaric acid. Figure S8a. Neighbour joining clustering with KEGG enzyme results of thermophilic samples on day 28. Figure S8b. Heatmap using KEGG orthology counts of the most abundant and most differently expressed enzymes of thermophilic controls and HCL samples of day 28. Figure S8c. KEGG orthology counts per samples of the enzyme subunits K00282 (glycin dehydrogenase (gcvP) subunit 1) and K00283 (gcvP subunit 2) as well of K01990 (ABC-2 type transport system ATP-binding protein) of thermophilic controls and HCL samples of day 28. Text S1. Piphillin results. (DOCX 7742 kb)