title: ÐÐ¾ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¸Ðµ Ð³ÐµÐ½Ð½ÑÑ ÑÐµÐ³ÑÐ»ÑÑÐ¾ÑÐ½ÑÑ ÑÐµÑÐµÐ¹ Ð¿Ð¾ Ð¸Ð½ÑÐµÐ³ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¼ Ð´Ð°Ð½Ð½ÑÐ¼ ÑÐ°Ð·Ð½Ð¾Ð¹ Ð¼Ð¾Ð´Ð°Ð»ÑÐ½Ð¾ÑÑÐ¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Reconstruction of gene regulatory networks based on integrated data of different modality
Creators: name: ÐÐ½Ð¾Ð´ÐµÐ»Ñ, Ð®Ð»Ð¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: ÐÐ°ÑÐµÐ¼Ð°ÑÐ¸ÐºÐ° Ð¿ÑÐ¸ÐºÐ»Ð°Ð´Ð½Ð°Ñ
ÐÐ½ÑÐ¾ÑÐ¼Ð°ÑÐ¸ÐºÐ°
Ð¼ÑÐ»ÑÑÐ¸Ð¾Ð¼Ð¸ÐºÑÐ½ÑÐµ Ð´Ð°Ð½Ð½ÑÐµ
ÑÐ¾Ð²Ð¼ÐµÑÑÐ½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð·
Ð°Ð½Ð°Ð»Ð¸Ð· Ð¾Ð±Ð¾Ð³Ð°ÑÐµÐ½Ð½Ð¾ÑÑÐ¸
ÑÑÐ·Ð°ÑÐ¸Ð¾Ð·
Ð»ÐµÐ½
Ð¼ÐµÑÐ¸Ð»ÑÑÐ¸Ñ
Ð³ÐµÐ½Ð½Ð°Ñ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ
ÑÐµÐ³ÑÐ»ÑÑÐ¾ÑÐ½ÑÐµ ÑÐµÑÐ¸ Ð³ÐµÐ½Ð¾Ð²
multiomics data
integrative analysis
enrichment analysis
fusarium wilt
flax
methylation
gene expression
gene regulatory networks

description: Ð Ð°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð½ÑÐµÐ³ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð¼Ñ Ð°Ð½Ð°Ð»Ð¸Ð·Ñ Ð¼ÑÐ»ÑÑÐ¸Ð¾Ð¼Ð¸ÐºÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð¸ Ð¼ÐµÑÐ¸Ð»ÑÑÐ¸Ð¸ Ð»ÑÐ½Ð°, Ð·Ð°ÑÐ°Ð¶ÐµÐ½Ð½Ð¾Ð³Ð¾ ÑÑÐ·Ð°ÑÐ¸Ð¾Ð·Ð¾Ð¼, Ð´Ð»Ñ Ð²ÑÑÐ²Ð»ÐµÐ½Ð¸Ñ Ð³ÐµÐ½Ð½ÑÑ ÑÐµÐ³ÑÐ»ÑÑÐ¾ÑÐ½ÑÑ ÑÐµÑÐµÐ¹ Ð¸ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° ÑÐ²ÑÐ·Ð°Ð½Ð½ÑÑ Ñ Ð½Ð¸Ð¼Ð¸ Ð¿ÑÐ¾ÑÐµÑÑÐ¾Ð². ÐÐ½Ð°Ð»Ð¸Ð· Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»ÑÑ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð¿Ð¾Ð´ÑÐ¾Ð´Ð¾Ð², Ð¾ÑÐ»Ð¸ÑÐ°ÑÑÐ¸ÑÑÑ ÑÐ¿Ð¾ÑÐ¾Ð±Ð¾Ð¼ Ð¸Ð½ÑÐµÐ³ÑÐ°ÑÐ¸Ð¸ Ð´Ð°Ð½Ð½ÑÑ, Ñ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸ÐµÐ¼ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¸ Ð½ÐµÐ¿Ð°ÑÐ°Ð¼ÐµÑÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð°Ð½Ð°Ð»Ð¸Ð·Ð°. Ð ÑÐ°Ð¼ÐºÐ°Ñ Ð¿ÐµÑÐ²Ð¾Ð³Ð¾ Ð¿Ð¾Ð´ÑÐ¾Ð´Ð° Ð´Ð°Ð½Ð½ÑÐµ Ð¿Ð¾ Ð¼ÐµÑÐ¸Ð»ÑÑÐ¸Ð¸ Ð¸ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð² Ð½ÐµÐ·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾ Ð¸Ð½ÑÐµÐ³ÑÐ¸ÑÐ¾Ð²Ð°Ð»Ð¸ÑÑ Ð² Ð¾Ð±ÑÐµÐ´Ð¸Ð½ÑÐ½Ð½ÑÑ Ð²ÑÐ±Ð¾ÑÐºÑ, Ðº ÐºÐ¾ÑÐ¾ÑÐ¾Ð¹ Ð¿ÑÐ¸Ð¼ÐµÐ½ÑÐ»ÑÑ Ð¼ÐµÑÐ¾Ð´ Ð½ÐµÐ¿Ð°ÑÐ°Ð¼ÐµÑÑÐ¸ÑÐµÑÐºÐ¸Ñ ÐºÐ¾Ð¼Ð±Ð¸Ð½Ð°ÑÐ¸Ð¹ Ð´Ð»Ñ Ð²ÑÑÐ²Ð»ÐµÐ½Ð¸Ñ Ð³ÐµÐ½Ð¾Ð², Ð´ÐµÐ¼Ð¾Ð½ÑÑÑÐ¸ÑÑÑÑÐ¸Ñ Ð·Ð½Ð°ÑÐ¸Ð¼ÑÐµ Ð¾ÑÐ»Ð¸ÑÐ¸Ñ Ð¿ÑÐ¸ Ð·Ð°ÑÐ°Ð¶ÐµÐ½Ð¸Ð¸ Ð»ÑÐ½Ð°. Ð ÑÐ°Ð¼ÐºÐ°Ñ Ð²ÑÐ¾ÑÐ¾Ð³Ð¾ Ð¿Ð¾Ð´ÑÐ¾Ð´Ð° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÐ¸ÑÐµÑÐºÐ¸Ðµ Ð¼ÐµÑÐ¾Ð´Ñ Ð´Ð»Ñ Ð¿Ð¾ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° ÑÐ°Ð·Ð½ÑÑ Ð¼Ð¾Ð´Ð°Ð»ÑÐ½Ð¾ÑÑÐµÐ¹: Ð²Ð²Ð¾Ð´Ð¸Ð»Ð¾ÑÑ Ð¿ÑÐµÐ´Ð¿Ð¾Ð»Ð¾Ð¶ÐµÐ½Ð¸Ðµ Ð¾ ÑÑÑÐµÑÑÐ²Ð¾Ð²Ð°Ð½Ð¸Ð¸ Ð³ÐµÐ½Ð¾Ð²-Ð´ÑÐ°Ð¹Ð²ÐµÑÐ¾Ð², ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸Ð·ÑÑÑÐ¸ÑÑÑ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸ÐµÐ¼ Ð¿Ð°ÑÑÐµÑÐ½Ð¾Ð² Ð¼ÐµÑÐ¸Ð»ÑÑÐ¸Ð¸ Ð¿ÑÐ¸ Ð·Ð°ÑÐ°Ð¶ÐµÐ½Ð¸Ð¸, Ð·Ð°ÑÐµÐ¼ Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»ÑÑ Ð°Ð½Ð°Ð»Ð¸Ð· Ð´Ð°Ð½Ð½ÑÑ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð´Ð»Ñ Ð²ÑÑÐ²Ð»ÐµÐ½Ð¸Ñ Ð³ÐµÐ½Ð¾Ð²-Ð¼Ð¸ÑÐµÐ½ÐµÐ¹, ÑÐµÐ³ÑÐ»Ð¸ÑÑÐµÐ¼ÑÑ Ð³ÐµÐ½Ð°Ð¼Ð¸-Ð´ÑÐ°Ð¹Ð²ÐµÑÐ°Ð¼Ð¸. ÐÐ±Ð° Ð¿Ð¾Ð´ÑÐ¾Ð´Ð° Ð²ÑÑÐ²Ð¸Ð»Ð¸ Ð¿Ð¾Ð²ÑÑÐµÐ½Ð½ÑÑ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð³ÐµÐ½Ð¾Ð² ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾Ð³Ð¾ Ðº Ð·Ð°ÑÐ°Ð¶ÐµÐ½Ð¸Ñ ÑÐ¾ÑÑÐ° Ð½Ð° 3 Ð´ÐµÐ½Ñ Ð¿Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ Ñ Ð¾Ð±Ð¾Ð¸Ð¼Ð¸ ÑÐ¾ÑÑÐ°Ð¼Ð¸ Ð½Ð° 5 Ð´ÐµÐ½Ñ. ÐÐµÐ½Ñ-Ð¼Ð¸ÑÐµÐ½Ð¸, Ð½Ð°Ð¹Ð´ÐµÐ½Ð½ÑÐµ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð²ÑÐ¾ÑÐ¾Ð³Ð¾ Ð¿Ð¾Ð´ÑÐ¾Ð´Ð°, Ð´ÐµÐ¹ÑÑÐ²Ð¸ÑÐµÐ»ÑÐ½Ð¾ ÑÑÐ°ÑÑÐ²Ð¾Ð²Ð°Ð»Ð¸ Ð² Ð²Ð°Ð¶Ð½ÑÑ Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð¿ÑÐ¾ÑÐµÑÑÐ°Ñ, ÑÑÐ¾ Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÐµÑ ÑÑÐ²ÐµÑÐ¶Ð´Ð°ÑÑ Ð¾Ð± ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð²Ð²ÐµÐ´ÐµÐ½Ð½Ð¾Ð¹ ÑÑÐµÐ¼Ñ Ð¾ÑÐ±Ð¾ÑÐ° Ð³ÐµÐ½Ð¾Ð²-Ð´ÑÐ°Ð¹Ð²ÐµÑÐ¾Ð². ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð¿Ð¾Ð»Ð½ÐµÐµ ÑÐ°ÑÐºÑÑÐ²Ð°ÑÑ Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÑ Ð¿ÑÐ¾ÑÐµÑÑÐ¾Ð² Ð°ÐºÑÐ¸Ð²Ð½ÑÑ Ð² Ð·Ð°ÑÐ¸ÑÐ½ÑÑ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð°Ñ ÑÐ°ÑÑÐµÐ½Ð¸Ñ, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÑÑÑ Ð²Ð¾Ð²Ð»ÐµÑÐµÐ½Ð½ÑÐµ Ð² Ð½Ð¸Ñ Ð³ÐµÐ½Ñ.
description: The work is devoted to the integrated analysis of multiomics expression and methylation data of flax infected with fusarium to identify gene regulatory networks and analyze the processes associated with them. The analysis was carried out using approaches that differ in the way of data integration, using parametric and nonparametric methods of analysis. In the first approach, methylation and expression data of genes were independently integrated into a combined sample, to which the method of Non-Parametric Combination was applied to identify genes showing significant differences in case of infection. In the second approach, parametric methods were used for sequential analysis of different modalities: the assumption of the existence of driver genes characterized by changes in methylation patterns during infection was introduced, then the expression data was analyzed to identify target genes regulated by driver genes. Both approaches revealed increased activity of the genes of the infection-resistant specie on day 3 compared to both species on day 5. The target genes found using the second approach actually participated in important biological processes, which allows to assert the effectiveness of the introduced scheme for selecting driver genes. The results obtained reveal more fully the dynamics of the processes active in the plants defense mechanisms, as well as determine the genes involved in them.