<b>Evaluation of the efficacy of Dettol in the Libyan market and its antimicrobial activity with </b><b><i>Artemisia spp</i></b><b> extract.</b>
Creators: name: Abushiba, Mahmoud
name: A. Alnuori, Buthuyna
name: A. Almred, Abdalmnam
Publisher: name: figshare
Year: 2024
Subjects: classificationCode: 420202
subject: Disease surveillance

description: <b>ABSTRACT</b><b>Background</b>: Dettol® is commonly used in households and healthcare facilities for multiple purposes, such as disinfecting skin, tools, equipment, and surfaces. Numerous studies have demonstrated that ineffective disinfectants can lead to the development of antibiotic-resistant strains. <b>Objective</b>: The study aims to assess the efficacy of Dettol products in the Libyan market and investigate the synergistic effects between Dettol and <i>Artemisia spp</i>. <b>Methods</b>: The agar diffusion methods were used to determine antibacterial action. The serial dilution method was used to estimate the minimum inhibitory concentration. Agar diffusion using cross-strips previously saturated with antibacterial compounds was used to estimate the synergistic activity. Dettol emulsion was used to determine the quality and chemical stability of products. <b>Result</b>: The antibacterial activity of Dettol (A) exhibited superior effectiveness against Gram-positive bacteria. Both aqueous and alcoholic extracts of <i>Artemisia spp</i> demonstrated antimicrobial properties against Gram-positive bacteria but had no impact on Gram-negative. The combination of Dettol and a plant extract produced an antagonistic activity. Stability tests on an emulsified suspension of Dettol's chemical components revealed that Dettol (A) maintained stability as a milky emulsion for several days, highlighting its superiority among the different types. <b>Conclusion</b>: Based on the study, we can infer that Dettol products exhibited varying inhibitory activity against tested bacteria, with Dettol (A) standing out due to its chemical stability and superior biological action. Low-quality antiseptics and disinfectants can lead to a rise in nosocomial infections, especially among Gram-negative bacteria such as <i>Pseudomonas aeruginosa</i>.<b>KEYWORDS</b>Dettol, <i>Artemisia spp.</i>, MIC, Synergistic activity, Gram-positive, Gram-negative bacteria, Antimicrobial resistant, <i>Pseudomonas spp</i>.<b>What is known about this topic</b>Misuse of disinfectants and antiseptics may result in negative outcomes, like the emergence of Resistant Microorganisms. Overuse or improper use can foster antimicrobial resistance in bacteria and other microorganisms within medical and community settings, complicating the treatment and management of infections. Inadequate use, such as neglecting recommended contact times or dilution ratios, can diminish their germ-killing efficacy and infection prevention.<b>What this paper adds</b>This study revealed that some Dettol products in the Libyan market lack effectiveness. The common practice of mixing it with some products or plant extracts to enhance its efficacy and fragrance, as circulated locally, diminishes its effectiveness. Poorly made disinfectants and antiseptics contribute to the spread of drug-resistant strains, especially in nosocomial infections.