Lophiostoma khanzada-kirgizbaeva Aluth., Gafforov & K. D. Hyde 2022, sp. nov.
Creators: name: Aluthmuhandiram, Janith V. S.
name: Wanasinghe, Dhanushka N.
name: Chethana, K. W. Thilini
name: Gafforov, Yusufjon
name: Saichana, Natsaran
name: Li, Xing Hong
name: Yan, Jiye
name: Mamarakhimov, Oybek M.
Publisher: name: Zenodo
Year: 2022
Subjects: Biodiversity
Taxonomy
Fungi
Ascomycota
Dothideomycetes
Pleosporales
Lophiostomataceae
Lophiostoma
Lophiostoma khanzada-Kirgizbaeva

description: Lophiostoma khanzada-kirgizbaeva Aluth., Gafforov & K.D. Hyde, sp. nov.
Index Fungorum number: IF 558817; Facesoffungi number: FoF 10528, FIGURE 2.
Etymology: khanzada-kirgizbaeva (Lat.) in honour of Uzbekistan mycologist Dr. Khanzada Kirgizbaeva (1937-2007) for her contribution in studying aquatic microfungi of Uzbekistan.
Saprobic on unknown hosts in terrestrial habitats. Sexual morph: Ascomata 170–450 μm high × 150–400 μm diam. (x̅ = 226 × 259 μm, n = 10), scattered to gregarious, immersed, dark brown to black, surrounded by a small blackened pseudoclypeus, globose to subglobose, ostiolate. Ostioles 120–140 μm high × 20–30 μm wide (x̅ = 130 × 25 μm, n = 5), slit-like, central, with a reduced crest and a pore-like opening. Peridium 35–80 μm wide (x̅ = 54 μm, n = 20), wider at the apex, and thinner at the base, composed of two strata, outer stratum comprising several layers of dark brown to black, somewhat flattened cells of textura angularis, fusing and indistinguishable from the host tissues, inner stratum comprising several layers with lightly pigmented cells of textura angularis. Hamathecium comprising 1–1.5 μm wide, septate, branched, pseudoparaphyses, situated between and above the asci. Asci 80–150 × 10–18 μm (x̅ = 113 × 15 μm, n = 30), 8-spored, bitunicate, fissitunicate, cylindric-clavate, with a long pedicel, rounded at the apex. Ascospores 15–25 × 6–8μm (x̅ = 22 × 7 μm, n = 50), uniseriate to bi-seriate, overlapping, initially hyaline and turning dark brown at maturity, fusiform with narrow rounded ends at immature state and turns ellipsoidal at maturity, 1–3 septate, constricted at the septum, widest at the region above or below the central septum, smooth-walled, guttulate, with mucilaginous sheath present in immature ascospores. Asexual morph: Undetermined.
Material examined: Uzbekistan, Jizzakh Region, Zaamin District, Zaamin National Nature Park, Supa Plateau in the Turkestan ridge of Pamir-Alay Mountains, on a dead branch of Rosa sp., 15 July 2019, Yusufjon Gafforov, YGZ75-1_1 (TASM 6158, holotype); ibid. on a dead stem of Rosa sp., YG-Z75-1_2 (TASM 6164, paratype).
Notes: Lophiostoma khanzada-kirgizbaeva (TASM 6158) is phylogenetically closest to L. paramacrostomum (MFLUCC 11-0463). Our strain L. khanzada-kirgizbaeva (TASM 6158) composed of globose to subglobose ascomata while L. paramacrostomum (MFLUCC 11-0463) composed of subglobose to conical ascomata (Thambugala et al. 2015). Lophiostoma khanzada-kirgizbaeva (TASM 6158) consists of 1–3 septate, fusiform, hyaline ascospores, which turn ellipsoidal and dark brown at maturity. Lophiostoma paramacrostomum (MFLUCC 11-0463) is characterized by 1-septate, hyaline, fusiform ascospores with narrow, acute ends. The phylogenetic analysis of combined SSU, ITS, LSU, tef 1-α, rpb 2 sequence data of Lophiostomataceae species revealed our strain Lophiostoma khanzada-kirgizbaeva (TASM 6158) within the Lophiostoma clade with good statistical support (97% ML-BS/0.91 BI-PP, FIGURE 1). Hence, confirming Lophiostoma khanzada-kirgizbaeva (TASM 6158) as a new species.
description: Published as part of Aluthmuhandiram, Janith V. S., Wanasinghe, Dhanushka N., Chethana, K. W. Thilini, Gafforov, Yusufjon, Saichana, Natsaran, Li, Xing Hong, Yan, Jiye & Mamarakhimov, Oybek M., 2022, Lophiostomataceae (Dothideomycetes): Introducing Lophiostoma khanzadakirgizbaeva sp. nov. and Paucispora xishanensis sp. nov., pp. 247-262 in Phytotaxa 559 (3) on page 253, DOI: 10.11646/phytotaxa.559.3.3, http://zenodo.org/record/7021719