title: ÐÐ¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾ÑÑÑ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ñ ÐºÐ°Ð·ÐµÐ¸Ð½Ð° ÐºÐ¾ÑÐ¾Ð²Ñ Ð´Ð»Ñ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÐºÐ°Ð»ÑÐ¿Ð°Ð¸Ð½Ð¾Ð² ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° Ð¸ ÐºÑÑÑÑ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Application of bovine casein to analyze the activity of human and rat calpains
Creators: name: Ð¢ÑÑÐ´Ð¸ÐµÐ², ÐÐµÐºÑÐ¾Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: ÐºÐ°Ð»ÑÐ¿Ð°Ð¸Ð½Ñ
ÐºÐ°Ð·ÐµÐ¸Ð½
Ð·Ð¸Ð¼Ð¾Ð³ÑÐ°ÑÐ¸Ñ
calpain
casein
zymography

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description: Â  Calpains are a family of intracellular proteolytic enzymes that, by cleaving their substrates, are involved in the regulation of all physiological functions. In the last decade, the activity of calpains in human blood has been considered as a diagnostic or prognostic marker for the development of a number of diseases of the nervous system, so it is necessary to develop a simple and cheap method for the determination of calpain activity. However, it is necessary to take into account that the most informative are methods that allow to determine the activity of different calpain isoforms. Therefore, the aim of the study was: to substantiate the possibility of applying the method of casein zymography in gel using cow beta-casein to analyze the activity of human and rat calpains. In this work we have shown that there are at least seven overlapping potential calpain cleavage sites in the amino acid sequence of bovine, rat and human beta-casein. The cleavage of these sites produces protein fragments not more than 60 amino acids in length; the proteolysis products are washed out of PAGE during casein zymography in gel according to the generally accepted protocol. Based on our data, we can conclude that casein isolated from cows milk can be used to analyze the activity of human and rat calpains by casein zymography in gel.