Additional file 1 of Tunable expression rate control of a growth-decoupled T7 expression system by l-arabinose only
Creators: name: Stargardt, Patrick
name: Striedner, Gerald
name: Mairhofer, Juergen
Publisher: name: figshare
Year: 2021
Subjects: subject: Biochemistry
subject: 29999 Physical Sciences not elsewhere classified
subject: FOS: Physical sciences
subject: FOS: Physical sciences
subject: Microbiology
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Genetics
subject: Molecular Biology
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Cancer

description: Additional file 1: Figure S1. Protein sequence of T7 RNA-polymerase found in strain BL21(DE3) compared to sequence found in strain BL21-AI. Figure S2. Flow cytometry analysis of single‑cell expression of GFPmut3.1 by strain BL21-AI during fed-batch like cultivation. Figure S3. Flow cytometry analysis of single‑cell expression of GFPmut3.1 by strain BL21-AI during fed-batch like cultivation. Figure S4. Flow cytometry analysis of single‑cell expression of GFPmut3.1 by strain BL21-AI during fed-batch like cultivation. Figure S5. Flow cytometry analysis of single‑cell expression of GFPmut3.1 by strain BL21-AI during fed-batch like cultivation. Figure S6. Flow cytometry analysis of single‑cell expression of GFPmut3.1 by strain BL21-AI during fed-batch like cultivation. Figure S7. Flow cytometry analysis of single‑cell expression of GFPmut3.1 by strain BL21-AI during fed-batch like cultivation. Figure S8. Flow cytometry analysis of single‑cell expression of GFPmut3.1 by strain BL21-AI during fed-batch like cultivation. Figure S9. Flow cytometry analysis of single‑cell expression of GFPmut3.1 by strain BL21-AI during fed-batch like cultivation. Figure S10. Flow cytometry analysis of strain BL21-AI, harboring no reporting plasmid, during fed-batch like cultivation. Figure S11. Product formation kinetics and flow cytometry analysis of single‑cell expression of E. coli strains BL21-AI (a, b, c, g, h, i) and BL21-AI (d, e, f, j, k, l) expressing Yhdy-GFP fusion protein during fed-batch like cultivation. Figure S12. Process characteristic showing product formation kinetics and flow cytometry analysis of E. coli strains BL21-AI (a, b, c) and BL21-AI (d, e, f) expressing Yhdy-GFP fusion protein during fed-batch like cultivation. Induction was performed with 100 mM l-arabinose and 100 mM l-arabinose + 1 mM IPTG. The mean CDM [mg] and mean specific GFP yield [rfu/mg] represents duplicate samples. Figure S13. Product formation kinetics and flow cytometry analysis of single‑cell expression of E. coli strains BL21-AI (a, b, c, g, h, i) and BL21-AI (d, e, f, j, k, l) expressing PstA-GFP fusion protein during fed-batch like cultivation. Figure S14. Process characteristic showing product formation kinetics and flow cytometry analysis of E. coli strains BL21-AI (a, b, c) and BL21-AI (d, e, f) expressing PstA-GFP fusion protein during fed-batch like cultivation. Figure S15. Product formation kinetics and flow cytometry analysis of single‑cell expression of E. coli strains BL21-AI (a, b, c, g, h, i) and BL21-AI (d, e, f, j, k, l) expressing YliF-GFP fusion protein during fed-batch like cultivation. Figure S16. Process characteristic showing product formation kinetics and flow cytometry analysis of E. coli strains BL21-AI (a, b, c) and BL21-AI ( d, e, f) expressing YliF-GFP fusion protein during fed-batch like cultivation. Figure S17. Product formation kinetics and flow cytometry analysis of single‑cell expression of E. coli strains BL21-AI (a, b, c, g, h, i) and BL21-AI (d, e, f, j, k, l) expressing YdiK-GFP fusion protein during fed-batch like cultivation. Figure S18. Process characteristic showing product formation kinetics and flow cytometry analysis of E. coli strains BL21-AI (a, b, c) and BL21-AI (d, e, f) expressing YdiK-GFP fusion protein during fed-batch like cultivation. Figure S19. Product formation kinetics and flow cytometry analysis of single‑cell expression of E. coli strains BL21-AI (a, b, c, g, h, i) and BL21-AI ( d, e, f, j, k, l) expressing YhhJ-GFP fusion protein during fed-batch like cultivation. Figure S20. Process characteristic showing product formation kinetics and flow cytometry analysis of E. coli strains BL21-AI (a, b, c) and BL21-AI (d, e, f) expressing YhhJ-GFP fusion protein during fed-batch like cultivation. Figure S21. Product formation kinetics and flow cytometry analysis of single‑cell expression of E. coli strains BL21-AI (a, b, c, g, h, i) and BL21-AI (d, e, f, j, k, l) expressing YfbF-GFP fusion protein during fed-batch like cultivation. Figure S22. Process characteristic showing product formation kinetics and flow cytometry analysis of E. coli strains BL21-AI (a, b, c) and BL21-AI (d, e, f) expressing YfbF-GFP fusion protein during fed-batch like cultivation. Figure S23. Western Blot showing overexpression of YhdY-GFP during fed-batch cultivations of E. coli strain BL21-AIand BL21-AI . Figure S24. Western Blot showing overexpression of PstA-GFP during fed-batch cultivations of E. coli strain BL21-AI and BL21-AI . Figure S25. Western Blot showing overexpression of YliF-GFP during fed-batch cultivations of E. coli strain BL21-AI and BL21-AI . Figure S26. Western Blot showing overexpression of YdiK-GFP during fed-batch cultivations of E. coli strain BL21-AI and BL21-AI . Figure S27. Western Blot showing overexpression of YfbF-GFP during fed-batch cultivations of E. coli strain BL21-AI and BL21-AI . Figure S28. Western Blot showing overexpression of YhhJ-GFP during fed-batch cultivations of E. coli strain BL21-AI and BL21-AI.