title: ÐÐ°Ð»Ð¸Ð´Ð°ÑÐ¸Ñ Ð¼Ð°ÑÐºÐµÑÐ½ÑÑ Ð¼Ð¾Ð»ÐµÐºÑÐ» Ð¼Ð¸ÐºÑÐ¾Ð ÐÐ Ð´Ð»Ñ Ð´Ð¸ÑÑÐµÑÐµÐ½ÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð¹ Ð´Ð¸Ð°Ð³Ð½Ð¾ÑÑÐ¸ÐºÐ¸ ÑÐ¾Ð»Ð»Ð¸ÐºÑÐ»ÑÑÐ½Ð¾Ð¹ Ð°Ð´ÐµÐ½Ð¾Ð¼Ñ Ð¸ ÑÐ¾Ð»Ð»Ð¸ÐºÑÐ»ÑÑÐ½Ð¾Ð¹ ÐºÐ°ÑÑÐ¸Ð½Ð¾Ð¼Ñ ÑÐ¸ÑÐ¾Ð²Ð¸Ð´Ð½Ð¾Ð¹ Ð¶ÐµÐ»ÐµÐ·Ñ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Validation of microRNA marker molecules for differential diagnostics of follicular adenoma and follicular carcinoma of thyroid gland
Creators: name: ÐÐ¾ÐºÑÐµÐ²Ð°, ÐÐ¾Ð»Ð¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð¼Ð¸ÐºÑÐ¾Ð ÐÐ
Ð½Ð¾Ð²Ð¾Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐ¸ÑÐ¾Ð²Ð¸Ð´Ð½Ð¾Ð¹ Ð¶ÐµÐ»ÐµÐ·Ñ
ÐÐ¢-ÐÐ¦Ð
Ð½Ð¾ÑÐ¼Ð°Ð»Ð¸Ð·Ð°ÑÐ¸Ñ
ROC-ÐºÑÐ¸Ð²ÑÐµ
microRNA
thyroid neoplasms
RT-PCR
normalization
ROC-curves

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description: This work is devoted to the assessment of diagnostic significance of the pool of microRNA molecules in samples of follicular adenoma and follicular carcinoma of the thyroid gland by OT-PCR. Tasks that were solved in the course of the work: 1. Study of scientific literature on the role of microRNAs in the diagnosis of oncologic diseases. 2. RNA isolation from clinical samples. 3. Analysis of expression of potentially marker microRNA molecules in clinical samples of thyroid gland by OT-PCR. 4. Selection of the most optimal strategy for normalization of OT-PCR results.Â  5. Evaluation of sensitivity and specificity of analyzing selected molecules in clinical samples by OT-PCR and sequencing methods. The objects of the study were formalin-fixed, paraffin-embedded (FFPE) samples of thyroid tissue. The subject of the study was quantitative characterization of marker microRNA expression in these samples. The work was performed at the scientific laboratory of subcellular technologies with the onco-endocrinology group of the N.N. Petrov National Medical Research Center of Oncology of the Ministry of Health of the Russian Federation. of the Minis-try of Health of the Russian Federation. In the course of the study, nucleic acids were isolated from clinical samples of thyroid tissue from patients with follicularÂ  adenoma and follicular carcinoma, analysis of the expression of selected microRNA molecules in the samples by OT-PCR, selection of the most optimal normalization method and ROC-analysis of the obtained results. Based on the obtained data, two microRNA molecules with the most pronounced marker properties were identified. The results of the work can be used in clinical practice for differential diagnostics of follicular adenoma and follicular thyroid carcinoma. When writing the literature review the following databases were used: eLIBRARY.RU, Scopus, NCBI. Statistical processing of experimental data was carried out using Excel 10.0, SigmaPlot 13.0, GraphPad Prizm 10.0 software package.