title: ÐÐ°ÑÑÑÐ°Ð±Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð¸ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ ÐºÑÐ»ÑÑÑÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð¡ÐÐ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Scaling and cultivation of CHO cell culture
Creators: name: ÐÐ°ÑÐ¸Ð»ÑÐµÐ², ÐÐ»ÐµÐºÑÐ°Ð½Ð´Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2025
Subjects: Ð¼Ð¾Ð½Ð¾ÐºÐ»Ð¾Ð½Ð°Ð»ÑÐ½ÑÐµ Ð°Ð½ÑÐ¸ÑÐµÐ»Ð°
n-Ð³Ð»Ð¸ÐºÐ¾Ð·Ð¸Ð»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´ÑÑÐ²Ð¾
ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
monoclonal antibodies
n-glycosylation
production
cultivation

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐµ ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ ÑÑÐ°Ð¿Ð¾Ð² ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð´Ð»Ñ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´ÑÑÐ²Ð° Ð¼Ð¾Ð½Ð¾ÐºÐ»Ð¾Ð½Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð°Ð½ÑÐ¸ÑÐµÐ»Ð° ÑÐµÑÑÐºÑÐ¸Ð¼Ð°Ð±. Ð ÑÐ°Ð¼ÐºÐ°Ñ Ð¿ÑÐ¾ÐµÐºÑÐ° ÑÐ°ÑÑÐ¼Ð¾ÑÑÐµÐ½Ñ Ð¿Ð¾Ð´Ð³Ð¾ÑÐ¾Ð²Ð¸ÑÐµÐ»ÑÐ½ÑÐµ ÑÑÐ°Ð´Ð¸Ð¸, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ðµ Ð¿Ð¾ÑÐµÐ²Ð½Ð¾Ð³Ð¾ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ð° Ð¸ Ð¿ÑÐ¾ÑÐµÑÑÑ Ð¿ÑÐ¾Ð´ÑÐºÑÐ¾Ð²Ð¾Ð³Ð¾ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ. ÐÑÐ½Ð¾Ð²Ð½ÑÐµ Ð·Ð°Ð´Ð°ÑÐ¸ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: 1. ÐÐ½Ð°Ð»Ð¸Ð· ÑÑÑÐµÑÑÐ²ÑÑÑÐµÐ¹ ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´ÑÑÐ²Ð° ÑÐµÐºÐ¾Ð¼Ð±Ð¸Ð½Ð°Ð½ÑÐ½Ð¾Ð³Ð¾ Ð¼Ð¾Ð½Ð¾ÐºÐ»Ð¾Ð½Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð°Ð½ÑÐ¸ÑÐµÐ»Ð° (Ð½Ð° Ð¿ÑÐ¸Ð¼ÐµÑÐµ ÑÐºÑÐ»Ð¸Ð·ÑÐ¼Ð°Ð±Ð°). 2. ÐÑÐ³Ð°Ð½Ð¸Ð·Ð°ÑÐ¸Ñ ÑÑÐ°Ð½ÑÑÐµÑÐ° ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¸ Ñ Ð´ÐµÑÐ°Ð»ÑÐ½ÑÐ¼ Ð¾Ð¿Ð¸ÑÐ°Ð½Ð¸ÐµÐ¼ ÑÑÐ°Ð¿Ð¾Ð² ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ, Ð²ÐºÐ»ÑÑÐ°Ñ: o ÐÑÐ¸Ð³Ð¾ÑÐ¾Ð²Ð»ÐµÐ½Ð¸Ðµ Ð¿Ð¸ÑÐ°ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ´Ñ, o ÐÐ¾Ð´Ð³Ð¾ÑÐ¾Ð²ÐºÑ Ð¿Ð¾ÑÐµÐ²Ð½Ð¾Ð³Ð¾ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ð°, o ÐÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð² Ð¾ÑÐµÐ²Ð¾Ð¼ Ð±Ð¸Ð¾ÑÐµÐ°ÐºÑÐ¾ÑÐµ. 3. ÐÑÐµÐ½ÐºÐ° Ð±ÐµÐ·Ð¾Ð¿Ð°ÑÐ½Ð¾ÑÑÐ¸ ÑÐ°Ð·ÑÐ°Ð±Ð°ÑÑÐ²Ð°ÐµÐ¼ÑÑ ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð¿ÑÐ¾ÑÐµÑÑÐ¾Ð². ÐÐ±ÑÐµÐºÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â Ð¿ÑÐ¾ÐµÐºÑ Ð¿Ð»Ð°Ð½Ð¸ÑÐ¾Ð²ÐºÐ¸ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´ÑÑÐ²ÐµÐ½Ð½Ð¾Ð³Ð¾ ÑÑÐ°ÑÑÐºÐ° Ð½Ð° Ð±Ð°Ð·Ðµ Ð´ÐµÐ¹ÑÑÐ²ÑÑÑÐµÐ³Ð¾ Ð±Ð¸Ð¾ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¿ÑÐµÐ´Ð¿ÑÐ¸ÑÑÐ¸Ñ. ÐÑÐ¾ÐµÐºÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð¾ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ Ð¼ÐµÑÐ¾Ð´Ð¸Ðº Ð¾ÑÐ´ÐµÐ»Ð° Ð³Ð»Ð°Ð²Ð½Ð¾Ð³Ð¾ Ð±Ð¸Ð¾ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð°, ÑÐ¿ÐµÑÐ¸Ð°Ð»Ð¸Ð·Ð¸ÑÑÑÑÐµÐ³Ð¾ÑÑ Ð½Ð° ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐµ ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¹ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´ÑÑÐ²Ð° Ð°ÐºÑÐ¸Ð²Ð½ÑÑ ÑÐ°ÑÐ¼Ð°ÑÐµÐ²ÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÑÐ±ÑÑÐ°Ð½ÑÐ¸Ð¹. Ð¡ÑÑÑÐºÑÑÑÐ° Ð´Ð¸Ð¿Ð»Ð¾Ð¼Ð½Ð¾Ð³Ð¾ Ð¿ÑÐ¾ÐµÐºÑÐ° Ð²ÐºÐ»ÑÑÐ°ÐµÑ: - ÐÐ¿Ð¸ÑÐ°Ð½Ð¸Ðµ Ð³Ð¾ÑÐ¾Ð²Ð¾Ð³Ð¾ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ° (ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ¸, Ð½Ð°Ð·Ð½Ð°ÑÐµÐ½Ð¸Ðµ); - ÐÐ±Ð¾ÑÐ½Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð²ÑÐ±Ð¾ÑÐ° ÑÑÐ°Ð¼Ð¼Ð°-Ð¿ÑÐ¾Ð´ÑÑÐµÐ½ÑÐ°; - ÐÐ¾Ð´Ð±Ð¾Ñ Ð¸ Ð°Ð½Ð°Ð»Ð¸Ð· ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´ÑÑÐ²Ð°; -ÐÑÐ°ÑÐºÑÑ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÑ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´ÑÑÐ²ÐµÐ½Ð½Ð¾Ð¹ Ð¿Ð»Ð¾ÑÐ°Ð´ÐºÐ¸; - Ð¢ÑÐµÐ±Ð¾Ð²Ð°Ð½Ð¸Ñ Ðº ÑÑÑÑÑ Ð¸ Ð¼Ð°ÑÐµÑÐ¸Ð°Ð»Ð°Ð¼; - ÐÐ»Ð¾Ðº-ÑÑÐµÐ¼Ñ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´ÑÑÐ²ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¿ÑÐ¾ÑÐµÑÑÐ°; - Ð¢ÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ ÑÑÐµÐ¼Ñ Ð¸ Ð¾Ð¿Ð¸ÑÐ°Ð½Ð¸Ðµ ÐºÐ»ÑÑÐµÐ²ÑÑ ÑÑÐ°Ð´Ð¸Ð¹; - ÐÐ°ÑÐµÑÐ¸Ð°Ð»ÑÐ½ÑÐµ ÑÐ°ÑÑÐµÑÑ ÑÑÐ°Ð¿Ð¾Ð²; - ÐÐ¾Ð´Ð±Ð¾Ñ Ð¾Ð±Ð¾ÑÑÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ñ Ð¾Ð±Ð¾ÑÐ½Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ ÑÐµÑÐµÐ½Ð¸Ð¹; - ÐÑÐµÐ½ÐºÑ Ð±ÐµÐ·Ð¾Ð¿Ð°ÑÐ½Ð¾ÑÑÐ¸ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´ÑÑÐ²Ð°. Ð Ð°Ð±Ð¾ÑÐ° Ð¾Ð±ÑÐµÐ´Ð¸Ð½ÑÐµÑ ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ðµ, ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ðµ Ð¸ ÑÐºÐ¾Ð½Ð¾Ð¼Ð¸ÑÐµÑÐºÐ¸Ðµ Ð°ÑÐ¿ÐµÐºÑÑ, Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð½ÑÐµ Ð½Ð° ÑÐ¾Ð·Ð´Ð°Ð½Ð¸Ðµ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ Ð¸ Ð±ÐµÐ·Ð¾Ð¿Ð°ÑÐ½Ð¾Ð³Ð¾ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´ÑÑÐ²Ð° Ð¼Ð¾Ð½Ð¾ÐºÐ»Ð¾Ð½Ð°Ð»ÑÐ½ÑÑ Ð°Ð½ÑÐ¸ÑÐµÐ». ÐÑÐ¸ Ð½Ð°Ð¿Ð¸ÑÐ°Ð½Ð¸Ð¸ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±Ð·Ð¾ÑÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ: eLIBRARY.RU, SCOPUS, NCBI. Ð¡ÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÑÑ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»Ð¸ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿Ð°ÐºÐµÑÐ° Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼: Microsoft Excel.
description: This work is devoted to the development of technological stages for the cultivation of cetuximab monoclonal antibody production. The project considers the preparatory stages, obtaining seed material, and the processes of food cultivation. Main research objectives: 1. Analysis of the existing technology for the production of recombinant monoclonal antibodies (on the example of eculizumab). 2. Organization of technology transfer with a detailed description of the cultivation stages, including: -Preparation of the nutrient medium, -Preparation of seed material, -Cultivation in an axial bioreactor. 3. Safety assessment of the developed technological processes. The object of research is the project of planning a production site based on an existing biotechnological enterprise. The design is based on the methods of the department of the chief biotechnologist, specializing in the development of technologies for the production of active pharmaceutical substances. The structure of the diploma project includes: - Description of the finished product (characteristics, purpose); -Justification for the selection of the producer strain; - Selection and analysis of production technology; - Brief description of the production site; - Requirements for raw materials and materials; - Flowchart of the production process; - Flow charts and description of key stages; - Material calculations of stages; - Selection of equipment with justification of decisions; - Production safety assessment. The work combines technical, technological, and economic aspects aimed at creating an effective and safe production of monoclonal antibodies. When writing the literature review, the following databases were used: eLibrary.RU, SCOPUS, NCBI. Statistical processing of experimental data was carried out using the software package: Microsoft Excel.