title: ÐÐµÑ Ð°Ð½Ð¸Ð·Ð¼Ñ ÑÐµÐ³ÑÐ»ÑÑÐ¸Ð¸ Ð´ÐµÐ»ÐµÐ½Ð¸Ñ Escherichia coli Ð² ÑÐ¾ÑÑÐ¾ÑÐ½Ð¸Ð¸ SOS-Ð¾ÑÐ²ÐµÑÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Escherichia coli cell division regulation mechanisms during the SOS response
Creators: name: Ð ÑÐ¼ÑÐ½ÑÐµÐ²Ð°, ÐÐ°ÑÐ°Ð»ÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: SOS-Ð¾ÑÐ²ÐµÑ
Escherichia coli
ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ðµ Ð´ÐµÐ»ÐµÐ½Ð¸Ðµ
SulA
FtsZ
ÑÐµÐºÐ²ÐµÑÑÑÐ°ÑÐ¸Ñ
ÐºÑÐ¿Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
SOS response
cell division
sequestration
capping

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾Ð³Ð¾ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð° Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð´ÐµÐ»ÐµÐ½Ð¸Ñ ÐºÐ»ÐµÑÐ¾Ðº Escherichia coli, Ð½Ð°ÑÐ¾Ð´ÑÑÐ¸ÑÑÑ Ð² ÑÐ¾ÑÑÐ¾ÑÐ½Ð¸Ð¸ SOS-Ð¾ÑÐ²ÐµÑÐ° Ð¸ Ð¸Ð·Ð¼ÐµÑÐµÐ½Ð¸Ñ Ð¼Ð¸Ð½Ð¸Ð¼Ð°Ð»ÑÐ½Ð¾Ð¹ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ Ð±ÐµÐ»ÐºÐ° SulA, Ð´Ð¾ÑÑÐ°ÑÐ¾ÑÐ½Ð¾Ð¹ Ð´Ð»Ñ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ Ð´ÐµÐ»ÐµÐ½Ð¸Ñ. ÐÐ»Ñ ÑÑÐ¾Ð³Ð¾ Ð² ÑÐ¾Ð´Ðµ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð° ÑÐ¾Ð·Ð´Ð°Ð½Ð° Ð¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð° ÐºÐ¾Ð½ÑÑÑÑÐºÑÐ¸Ñ, Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÑÑÐ°Ñ Ð¸Ð¼Ð¸ÑÐ¸ÑÐ¾Ð²Ð°ÑÑ SOS-Ð¾ÑÐ²ÐµÑ Ð² ÐºÐ»ÐµÑÐºÐ°Ñ E. coli Ð² Ð²Ð¸Ð´Ðµ Ð¾ÑÑÐ°Ð½Ð¾Ð²ÐºÐ¸ Ð´ÐµÐ»ÐµÐ½Ð¸Ñ. ÐÐ»Ñ Ð¸Ð·Ð¼ÐµÑÐµÐ½Ð¸Ñ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ Ð±ÐµÐ»ÐºÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ Ð½ÐµÐ·Ð°Ð²Ð¸ÑÐ¸Ð¼ÑÐµ Ð¼ÐµÑÐ¾Ð´Ñ, ÑÐ°ÐºÐ¸Ðµ ÐºÐ°Ðº Ð°Ð½Ð°Ð»Ð¸Ð· ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ Ð¿Ð¾ Ð¸Ð·Ð¼ÐµÑÐµÐ½Ð¸Ñ Ð¸Ð½ÑÐµÐ½ÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ ÑÐ»ÑÐ¾ÑÐµÑÑÐµÐ½ÑÐ¸Ð¸ Ð¸ Ð¸Ð¼Ð¼ÑÐ½Ð¾Ð±Ð»Ð¾ÑÑÐ¸Ð½Ð³.
Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð±ÑÐ»Ð¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ñ ÑÑÐµÐ´Ð½Ð¸Ðµ Ð·Ð½Ð°ÑÐµÐ½Ð¸Ñ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ Ð±ÐµÐ»ÐºÐ° SulA, Ð´Ð¾ÑÑÐ°ÑÐ¾ÑÐ½Ð¾Ð¹ Ð´Ð»Ñ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð´ÐµÐ»ÐµÐ½Ð¸Ñ. Ð¡ÑÐ°Ð²Ð½ÐµÐ½Ð¸Ðµ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ SulA Ð² ÐºÐ»ÐµÑÐºÐµ Ñ Ð¸Ð·Ð²ÐµÑÑÐ½Ð¾Ð¹ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸ÐµÐ¹ Ð±ÐµÐ»ÐºÐ° FtsZ Ð¿Ð¾Ð·Ð²Ð¾Ð»Ð¸Ð»Ð¾ ÑÑÑÐ°Ð½Ð¾Ð²Ð¸ÑÑ, ÑÑÐ¾ Ð´Ð»Ñ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð´Ð¾ÑÑÐ°ÑÐ¾ÑÐ½Ð¾ ÑÑÐ±ÑÑÐµÑÐ¸Ð¾Ð¼ÐµÑÑÐ¸ÑÐµÑÐºÐ¸Ñ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ² SulA. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð³Ð¾Ð²Ð¾ÑÑÑ Ð¾ ÑÐ¾Ð¼, ÑÑÐ¾ ÑÑÑÐµÑÑÐ²ÑÑÑÐ°Ñ Ð½Ð° ÑÐµÐ³Ð¾Ð´Ð½ÑÑÐ½Ð¸Ð¹ Ð´ÐµÐ½Ñ Ð¼Ð¾Ð´ÐµÐ»Ñ ÑÐµÐºÐ²ÐµÑÑÑÐ°ÑÐ¸Ð¸ Ð½Ðµ Ð¿Ð¾Ð»Ð½Ð¾ÑÑÑÑ Ð¾Ð±ÑÑÑÐ½ÑÐµÑ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð´ÐµÐ»ÐµÐ½Ð¸Ñ Ð±ÐµÐ»ÐºÐ¾Ð¼ SulA in vivo Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ Ð´Ð°Ð½Ð½Ð¾Ð³Ð¾ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°. ÐÑÐ´ÑÑÐ¸Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð´Ð¾Ð»Ð¶Ð½Ñ Ð¿Ð¾Ð¼Ð¾ÑÑ ÑÑÑÐ°Ð½Ð¾Ð²Ð¸ÑÑ, ÐºÐ°ÐºÐ°Ñ Ð¼Ð¾Ð´ÐµÐ»Ñ Ð±Ð»Ð¾ÐºÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð´ÐµÐ»ÐµÐ½Ð¸Ñ ÑÐ²Ð»ÑÐµÑÑÑ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ Ð°Ð´ÐµÐºÐ²Ð°ÑÐ½Ð¾Ð¹, Ð¸, ÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÐµÐ»ÑÐ½Ð¾, Ð»ÑÑÑÐµ Ð¿Ð¾Ð½ÑÑÑ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ñ SOS-Ð¾ÑÐ²ÐµÑÐ°, ÐºÐ¾ÑÐ¾ÑÑÐ¹ Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÐµÑ Ð±Ð°ÐºÑÐµÑÐ¸ÑÐ¼ Ð¿ÐµÑÐµÐ¶Ð¸ÑÑ Ð½ÐµÐ±Ð»Ð°Ð³Ð¾Ð¿ÑÐ¸ÑÑÐ½ÑÐµ Ð´Ð»Ñ Ð½Ð¸Ñ ÑÑÐ»Ð¾Ð²Ð¸Ñ.
description: The given work is devoted to study the mechanism of cell division inhibition in Escherichia coli during SOS response and to measure the minimal concentration of SulA protein in the cell, which is sufficient to block the cell division. During this work a construction was created and used which allowed us to mimick the SOS response in E. coli cells in terms of the cell division arrest. Measurements of protein concentration were carried out using several independent methods, such as fluorescent intensity quantification or semi-quantifying Western blotting.
As a result, SulA concentration values sufficient to inhibit division were determined. Comparison between obtained concentration of SulA and concentration of FtsZ well-known from publications showed that it is enough to add substoichiometric quantity of SulA for blocking cell division. The results indicate that the sequestration model is not comprehensive to explain in vivo mechanism of blocking division by the action of the SulA protein under the conditions of this experiment. Further study should help to find out which model is correct, and, thereafter, to better explain the action of SOS response mechanism, which allows bacteria to survive the adverse conditions.