cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Secular dynamics in hierarchical three-body systems with mass loss and mass transfer
Creators: name: Michaely, Erez
name: Perets, Hagai B.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Solar and Stellar Astrophysics (astro-ph.SR)
subject: Earth and Planetary Astrophysics (astro-ph.EP)
subject: FOS: Physical sciences

description: Recent studies have shown that secular evolution of triple systems can play a major role in the evolution and interaction of their inner binaries. Very few studies explored the stellar evolution of triple systems, and in particular the mass loss phase of the evolving stellar components. Here we study the dynamical secular evolution of hierarchical triple systems undergoing mass loss. We use the secular evolution equations and include the effects of mass-loss and mass-transfer, as well as general relativistic effects. We present various evolutionary channels taking place in such evolving triples, and discuss both the effects of mass-loss and mass-transfer in the inner binary system, as well as the effects of mass-loss/transfer from an outer third companion. We discuss several distinct types/regimes of triple secular evolution, where the specific behavior of a triple system can sensitively depend on its hierarchy and the relative importance of classical and general relativistic effects. We show that the orbital changes due to mass-loss and/or mass-transfer processes can effectively transfer a triple system from one dynamical regime to another. In particular, mass loss/transfer can both induce and quench high amplitude (Lidov-Kozai) variations in the eccentricity and inclination of the inner binaries of evolving triples. They can also change the system dynamics from an orderly periodic behavior to a chaotic one, and vice versa.
Additional file 1 of ARID1A-dependent maintenance of H3.3 is required for repressive CHD4-ZMYND8 chromatin interactions at super-enhancers
Creators: name: Reske, Jake J.
name: Wilson, Mike R.
name: Armistead, Brooke
name: Harkins, Shannon
name: Perez, Cristina
name: Hrit, Joel
name: Adams, Marie
name: Rothbart, Scott B.
name: Missmer, Stacey A.
name: Fazleabas, Asgerally T.
name: Chandler, Ronald L.
Publisher: name: figshare
Year: 2022
Subjects: subject: Genetics
subject: FOS: Biological sciences

description: Additional file 1: Supplementary Information. Figure S1. Supplemental ARID1A knockdown differential H3.3 data. Figure S2. H3.3 knockdown functional analysis. Figure S3. Effects of CHD4 loss on H3.3 chromatin and comparison to ARID1A. Figure S4. Additional chromatin features profiled in 12Z cells. Figure S5. Peptide specificity of anti-acetyl-H2A.Z (K4/K7). Figure S6. ChromHMM model optimization. Figure S7. Chromatin accessibility repressed by ARID1A is associated with H4 acetylation. Figure S8. Chromatin feature correlation across promoter-proximal super-enhancers. Figure S9. siCHD4/siZMYND8 functional analysis. Figure S10. H4K16ac enrichment at repressed mechanistic genes. Figure S11. Additive transcriptional repression by ARID1A and CHD4. Figure S12. Uncropped Western blots.
Recent spin physics results from PHENIX
Creators: name: Park, Sanghwa
Publisher: name: Zenodo
Year: 2023
Subjects: pic23
phenix
spin
polarization
gluon
transverse spin asymmetry
longitudinal spin asymmetry
p+p
pion
π0
photon
direct photon
W
asymmetry
η
heavy flavor
electron
p+al
p+au
forward-rapidity
hadron
mid-rapidity

description: Talk presented at the Physics in Collision 2023 conference
Expression of LMP1 in epithelial cells leads to the activation of a select subset of NF-kappa B/Rel family proteins.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: This study demonstrates that the Epstein-Barr virus protein LMP1 activates a specific subset of NF-kappa B/Rel proteins in the C33 epithelial cell line. Western immunoblot analysis used to analyze the intracellular distribution and abundance of the proteins present in these complexes demonstrated that levels of the p50 and p52 proteins were significantly elevated in the nuclei of LMP1-expressing cells. The data also suggest that LMP1 facilitates the translocation of p50 to the nucleus and may affect the processing of the p100 and p105 precursor proteins or the stability of p52 and p50.
Function of a 5'-end genomic RNA mutation that evolves during persistent mouse hepatitis virus infection in vitro.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: Persistently infected cultures of DBT cells were established with mouse hepatitis virus strain A59 (MHV-A59), and the evolution of the MHV leader RNA and 5' end of the genome was studied through 119 days postinfection. Sequence analysis of independent clones demonstrated an overall mutation frequency approaching 1.2 x 10(-3) to 6.7 x 10(-3). The rate of fixation of mutations was about 1.2 x 10(-5) to 7.6 x 10(-5) per nucleotide (nt) per day. In contrast to finding in bovine coronavirus, the MHV leader RNA sequences were extremely stable and did not evolve significantly during persistent infection. Rather, a 5' untranslated region (UTR) A-to-G mutation at nt 77 in the genomic RNA emerged by day 56 and accumulated until 50 to 80% of the genome-length molecules retained the mutation by 119 days postinfection. Although other 5'-end mutations were noted, only the nt 77 mutation was significantly associated with viral persistence in vitro. Mutations were also found in the 5' end of the p28 coding region, but no specific alterations accumulated in genome-length molecules through 119 days postinfection. The 5' UTR nt 77 mutation resulted in an 18-amino-acid open reading frame (ORF) upstream of the ORF 1a AUG start site. By in vitro translation assays, the small ORF was not translated into detectable product but the mutation significantly enhanced translation of the downstream p28 ORF about 2.5-fold. Variant viruses, containing either the nt 77 A-to-G mutation (V16-ATG+) or wild-type sequences at this locus (V1-ATG-), were isolated at 119 days postinfection. The variant viruses replicated more efficiently than wild-type virus and were extremely cytolytic in DBT cells, suggesting that the A-to-G mutation did not encode a nonlytic or attenuated phenotype. Consistent with the in vitro translation results, a significant increase (approximately 3.5-fold) in p28 expression was also observed with the mutant virus (V16-ATG+) in DBT cells compared with that in wild-type controls. These data indicate that MHV persistence was significantly associated with mutation and evolution in the 5'-end UTR which enhanced the translation of the ORF 1a and potentially ORF 1b polyproteins which function in virus transcription and replication.
Hepatitis delta virus mutant: effect on RNA editing.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: During the replication cycle of hepatitis delta virus (HDV), RNA editing occurs at position 1012 on the 1679-nucleotide RNA genome. This changes an A to G in the amber termination codon, UAG, of the small form of the delta antigen (delta Ag). The resultant UGG codon, tryptophan, allows the translation of a larger form of the delta Ag with a 19-amino-acid C-terminal extension. Using HDV cDNA-transfected cells, we examined the editing potential of HDV RNA mutated from G to A at 1011 on the antigenome, adjacent to normal editing site at 1012. Four procedures were used to study not only the editing of the A at 1012, but also that of the new A at 1011: (i) nucleotide sequencing, (ii) a PCR-based RNA-editing assay, (iii) immunoblot assays, and (iv) immunofluorescence. Five findings are reported. (i) Even after the mutation at 1011, editing still occurred at 1012. (ii) Site 1011 itself now acted as a novel RNA-editing site. (iii) Sites 1011 and 1012 were edited independently. (iv) At later times, both sites became edited, thereby allowing the synthesis of the large form of the delta Ag (delta Ag-L). (v) Via immunofluorescence, such double editing became apparent as a stochastic event, in that groups of cells arose in which the changes had taken place. Evaluation of these findings and of those from previous studies of the stability of the HDV genomic sequence (H.J. Netter et al., J. Virol. 69:1687-1692, 1995) supports both the recent reevaluation of HDV RNA editing as occurring on antigenomic RNA (Casey and Gerin, personal communication) and the interpretation that editing occurs via the RNA-modifying enzyme known as DRADA.
Interaction of the 72-kilodalton human cytomegalovirus IE1 gene product with E2F1 coincides with E2F-dependent activation of dihydrofolate reductase transcription.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: Three polypeptides are produced from the major immediate-early (IE) region of human cytomegalovirus by alternative splicing. The IE gene products regulate subsequent viral and cellular gene expression. We previously reported that cotransfection of a genomic clone of the major IE region stimulated transient expression of chloramphenicol acetyltransferase driven by the dihydrofolate reductase (DHFR) promoter and that an intact E2F site was required for the trans activation (M. Wade, T. F. Kowalik, M. Mudryj, E.-S. Huang, and J. C. Azizkhan, Mol. Cell. Biol. 12:4364-4374, 1992). With the availability of cDNA clones for the individual major IE proteins, we sought to determine which of these proteins exerted this effect and whether the IE protein(s) interacted with E2F. In this study, we use cotransfection to demonstrate that the 55- and 86-kDa major IE proteins from the IE2 region can each moderately trans activate the DHFR promoter and that the 72-kDa IE1 protein stimulates DHFR transcription to a much higher level. Furthermore, trans activation through the 72-kDa IE1 protein is in part E2F dependent, while activation by the 55- and 86-kDa IE proteins is E2F independent. We also demonstrate by in vitro pull-down assays that the 72-kDa IE1 protein can specifically interact with the DNA binding domain of E2F1 (amino acids 88 to 191) in the presence of nuclear extract. Moreover, antibodies to either E2F1 or IE72 will immunoprecipitate both E2F and IE72 from cells that stably express IE72, and antibody to E2F1 will immunoprecipitate IE72 from normal human fibroblast cells infected with human cytomegalovirus.
Structural analyses of the Epstein-Barr virus BamHI A transcripts.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: Epstein-Barr virus (EBV) gene expression in nasopharyngeal carcinoma (NPC) includes abundant rightward transcription of the BamHI A fragment, consisting of mRNAs ranging in size from approximately 4.0 to 8.0 kb. These transcripts include several distinctly spliced forms which are 3'-end coterminal and contain the BamHI A rightward frame 0 (BARF0) open reading frame (ORF) in the final exon. BamHI A transcription is detected at a lower level of expression in EBV-infected lymphoid cells. In this study, cDNA cloning, reverse transcription-based PCR, and Northern (RNA) blotting were used to further define the structures of the BamHI A transcripts and to characterize their expression in different EBV-infected tissues. Three BamHI A cDNAs isolated from a passaged NPC represent previously unidentified mRNAs that contain BARF0 and additional ORFs encoded by multiple exons, including one which extends the size of the BARF0 ORF from 174 to 279 codons. The distinct exons were detected in multiple, differently sized mRNAs, indicating that these transcripts have complex patterns of alternate splicing. In support of this finding, 5'-end analysis confirmed the presence of a previously reported start site and also identified a subset of transcripts of 4.8 kb and larger that initiate further 5' to this site. In addition, 3'-end analysis identified heterogeneous 3'-end processing in all of the BamHI A mRNAs, resulting in transcripts that either contain the entire BARF0 ORF or are cleaved and polyadenylated 5' of the stop codon. Finally, the expression of multiple, distinctly spliced BamHI A transcripts was consistently detected in a wide range of EBV-infected samples, including NPC, Burkitt's lymphoma, and parotid carcinoma biopsy samples, and in type I and type III Burkitt's lymphoma lines and type III lymphoblastoid cell lines. This complex pattern of start site selection, alternate splicing, and heterogeneous 3'-end processing is likely to regulate the expression in vivo of the ORFs encoded by the EBV BamHI A transcripts.
Electrostatics-driven shape transitions in soft shells
Creators: name: Jadhao, Vikram
name: Thomas, Creighton K.
name: de la Cruz, Monica Olvera
Publisher: name: arXiv
Year: 2014
Subjects: subject: Soft Condensed Matter (cond-mat.soft)
subject: FOS: Physical sciences

description: Manipulating the shape of nanoscale objects in a controllable fashion is at the heart of designing materials that act as building blocks for self-assembly or serve as targeted drug delivery carriers. Inducing shape deformations by controlling external parameters is also an important way of designing biomimetic membranes. In this paper, we demonstrate that electrostatics can be used as a tool to manipulate the shape of soft, closed membranes by tuning environmental conditions such as the electrolyte concentration in the medium. Using a molecular dynamics-based simulated annealing procedure, we investigate charged elastic shells that do not exchange material with their environment, such as elastic membranes formed in emulsions or synthetic nanocontainers. We find that by decreasing the salt concentration or increasing the total charge on the shell's surface, the spherical symmetry is broken, leading to the formation of ellipsoids, discs, and bowls. Shape changes are accompanied by a significant lowering of the electrostatic energy and a rise in the surface area of the shell. To substantiate our simulation findings, we show analytically that a uniformly charged disc has a lower Coulomb energy than a sphere of the same volume. Further, we test the robustness of our results by including the effects of charge renormalization in the analysis of the shape transitions and find the latter to be feasible for a wide range of shell volume fractions.
description: 6 pages, 4 figures
Temperature-sensitive hepatitis A virus mutants with deletions downstream of the first pyrimidine-rich tract of the 5' nontranslated RNA are impaired in RNA synthesis.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: Hepatitis A virus (HAV) mutants containing large deletions within the first pyrimidine-rich tract (pY1; nucleotides [nt] 99 to 138) of the 5' nontranslated RNA (5'NTR) replicate well in cultured cells, while those with pY1 deletions which extend in a 3' direction to include nt 140 to 144 (CUUGU) have a temperature-sensitive (ts) replication phenotype (D.R. Shaffer, E.A. Brown, and S.M. Lemon, J. Virol. 68:5568-5578, 1994). To characterize this replication defect, the ts mutant delta 131-144 was grown under one-step conditions at the nonpermissive temperature (37 degrees C). A shift to the permissive temperature (31 degrees C) for the first 18 h of the viral replication cycle did not enhance virus yields, indicating that temperature sensitivity is not due to a defect in viral entry or uncoating. Similarly, absence of increased yield with a late shift to 31 degrees C between 54 and 72 h suggested that the ts defect does not involve viral assembly. Although monocistronic RNA transcripts containing the delta 99-144 deletion directed translation 22 to 58% less efficiently than the standard 5'NTR in transfected BS-C-1 cells, this difference was present at both 31 and 37 degrees C. In addition, there were no temperature-dependent differences in the abilities of bicistronic transcripts containing either ts or non-ts 5'NTR sequences within the intercistronic space to direct translation of a downstream reporter gene. Thus, ts mutations do not confer a demonstrable temperature-related defect in cap-independent translation. In contrast, an RNase protection assay showed that synthesis of viral plus-strand RNA was markedly delayed in BS-C-1 cells infected with ts virus at 37 degrees C. Analysis of the nucleotide sequence surrounding the deletion in a non-ts revertant derived from delta 116-144 virus revealed that a single U-to-G transversion at nt 114 (CUUUU-->CUUGU) had restored the sequence normally present between nt 140 and 144. These results indicate that ts mutants of HAV with deletions extending downstream from the pY1 domain to nt 140 to 144 are defective in RNA synthesis and that the single-stranded RNA segment containing nt 140 to 144 plays a critical role in replication of HAV RNA.
Archivio storico per le province napoletane. N.S. A.14, Vol. 53. dell’intera collezione, n.1-4 (30 maggio 1929)
Creators: name: Schipa, Michelangelo
name: Scandone, Adele
name: Croce, Benedetto
name: Martini, Ernesto M.
name: Lemmi, Francesco
name: Paladino, Giuseppe
name: Nicolini, Nicola
Publisher: name: Napoli : R. Deputazione napoletana di storia patria, 1928 [i.e.1929]
Year: 2020

description: N.S. A.14, Vol. 53. dell’intera collezione, n.1-4 (30 mag 1929): M. Schipa, Sicilia e Italia sotto Federico II di Svevia, P. 5-113 ; A. Scandone, Le tristi reyne di Napoli Giovanna III e Giovanna IV d'Aragona, P. 114-155 ; B. Croce, Un'inedita farsa cavaiola di Vincenzo Braca, P. 156-189 ; E. M. Martini, La vita e le opere di Camillo Tutini, P. 190-219 ; F. Lemmi, Nelson a Napoli nel giugno del 1799, P. 220-284 ; G. Paladino, La congiura del "Monaco" (1830-33), P. 285-387 ; N. Nicolini, La città di Napoli nell' anno della battaglia di Lepanto (dai dispacci del Residente Veneto), P. 388-422.
The E5 oncoprotein of human papillomavirus type 16 inhibits the acidification of endosomes in human keratinocytes.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: The human papillomavirus type 16 E5 oncoprotein possesses mitogenic activity that acts synergistically with epidermal growth factor (EGF) in human keratinocytes and inhibits the degradation of the EGF receptor in endosomal compartments after ligand-stimulated endocytosis. One potential explanation for these observations is that E5 inhibits the acidification of endosomes. This may be mediated through the 16-kDa component of the vacuolar proton-ATPase, since animal and human papillomavirus E5 proteins bind this subunit protein. Using a ratio-imaging technique to determine endosomal pH, we found that the acidification of endosomes in E5-expressing keratinocytes was delayed at least fourfold compared with normal human keratinocytes and endosomes in some cells never completely acidified. Furthermore, E5 expression increased the resistance of keratinocytes to protein synthesis inhibition by diphtheria toxin, a process dependent on efficient endosomal acidification. Finally, artificially inhibiting endosomal acidification with chloroquine during the endocytosis of EGF receptors in keratinocytes demonstrated many of the same effects as the expression of human papillomavirus type 16 E5, including prolonged retention of undegraded EGF receptors in intracellular vesicles.
Appendix_S1 from Correction to ‘A triple threat: high population density, high foraging intensity and flexible habitat preferences explain high impact of feral cats on prey’ 2022 by Hamer et al.
Creators: name: Hamer, Rowena P.
name: Gardiner, Riana Z.
name: Proft, Kirstin M.
name: Johnson, Christopher N.
name: Jones, Menna E.
Publisher: name: The Royal Society
Year: 2022
Subjects: subject: Ecology
subject: FOS: Biological sciences
subject: 60801 Animal Behaviour

description: Electronic supplementary material associated with http://dx.doi.org/10.1098/rspb.2022.1985
Controlled pore formation on mesoporous single crystalline silicon nanowires: threshold and mechanisms
Creators: name: Weidemann, Stefan
name: Kockert, Maximilian
name: Wallacher, Dirk
name: Ramsteiner, Manfred
name: Mogilatenko, Anna
name: Rademann, Klaus
name: Fischer, Saskia F.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Mesoscale and Nanoscale Physics (cond-mat.mes-hall)
subject: Materials Science (cond-mat.mtrl-sci)
subject: FOS: Physical sciences

description: Silicon nanowires are prepared by the method of the two-step metal-assisted wet chemical etching. We have analyzed the structure of solid, rough and porous nanowire surfaces of boron-doped silicon substrates with resistivities of ρ> 1000 Ωcm, ρ= 14-23 Ωcm, ρ< 0.01 Ωcm by scanning electron microscopy and nitrogen gas adsorption. Silicon nanowires prepared from highly-doped silicon reveal mesopores on their surface. However, we found a limit for pore formation. Pores were only formed by etching below a critical H$_2$O$_2$ concentration (c$_{\mbox{H}_2\mbox{O}_2}$ < 0.3 M). Furthermore, we have determined the pore size distribution in dependence on the etching parameters and characterized the morphology of the pores on the nanowire surface. The pores are in the regime of small mesopores with a mean diameter of 9-13 nm. Crystal and surface structure of individual mesoporous nanowires have been investigated by transmission electron microscopy. The vibrational properties of nanowire ensembles have been investigated by Raman spectroscopy. Heavily boron-doped silicon nanowires are highly porous and the remaining single crystalline silicon nanoscale mesh leads to a redshift and a strong asymmetric line broadening for Raman scattering by optical phonons at 520 cm$^{-1}$. This redshift, λ$_{\mbox{Si bulk}}$ = 520 cm$^{-1}$ $\rightarrow$ λ$_{\mbox{Si nanowire}}$ = 512 cm$^{-1}$, hints to a phonon confinement in mesoporous single crystalline silicon nanowire.
description: 16 pages, 16 figures
The Epstein-Barr virus latent membrane protein 1 induces expression of the epidermal growth factor receptor.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: The Epstein-Barr virus (EBV)-encoded LMP1 protein is an important component of the process of transformation by EBV. LMP1 is essential for transformation of B lymphocytes, most likely because of its profound effects on cellular gene expression. Although LMP1 is expressed in the majority of nasopharyngeal carcinoma (NPC) tumors, the effect of LMP1 on cellular gene expression and its contribution to the development of malignancy in epithelial cells is largely unknown. In this study the effects of LMP1 on the expression and tyrosine kinase activity of the epidermal growth factor receptor (EGFR) were investigated in C33A human epithelial cells. Stable or transient expression of LMP1 in C33A cells increased expression of the EGFR at both the protein and mRNA levels. In contrast, expression of the EGFR was not induced by LMP1 in EBV-infected B lymphocytes. Stimulation of LMP1-expressing C33A cells with epidermal growth factor (EGF) caused rapid tyrosine phosphorylation of the EGFR (pp170) as well as several other proteins, including pp120, pp85, pp75, and pp55, indicating that the EGFR induced by LMP1 is functional. LMP1 also induced expression of the A20 gene in C33A epithelial cells. In C33A cells, LMP1 expression increased the proliferative response to EGF, as LMP1-expressing C33A cells continued to increase in number when plated in serum-free media supplemented with EGF, while the neo control cells exhibited very low levels of viability and did not proliferate. Immunoblot analysis of protein extracts from nude mouse-passaged NPC tumors also demonstrated that the EGFR is overexpressed in primary NPC tumors as well as those passaged in nude mice. This study suggests that the alteration in the growth patterns of C33A cells expressing LMP1 is a result of increased proliferative signals due to enhanced EGFR expression, as well as protection from cell death due to LMP1-induced A20 expression. The induction of EGFR and A20 by LMP1 may be an important component of EBV infection in epithelial cells and could contribute to the development of epithelial malignancies such as NPC.
A functional hepatocyte nuclear factor 3 binding site is a critical component of the duck hepatitis B virus major surface antigen promoter.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: The gene coding for the S protein, the smaller of the two envelope antigens of the duck hepatitis B virus (DHBV), is transcribed from a TATA-less promoter. In this study, we localized the promoter to a 245-bp segment of the genome that was capable of efficiently driving expression of a linked reporter gene upon transient transfection into the differentiated hepatoma cell lines LMH and HepG2. However, no measurable activity from this construct could be detected in similar assays with the dedifferentiated cell line HepG2.1 or the nonhepatic cell line HeLa. Located at position -25 relative to the transcriptional start site was a sequence conforming to the consensus binding site for hepatocyte nuclear factor 3 (HNF3). Deletion of this region reduced activity of the reporter gene to barely detectable levels in LMH cells. The results of electrophoretic mobility shift analysis (EMSA) demonstrated that a double-stranded oligonucleotide containing this sequence formed a specific complex with DNA-binding proteins from LMH and HepG2 cells but not with nuclear extracts obtained from HepG2.1 or HeLa cells. Cotransfection of HepG2.1 cells with DHBV S promoter constructs and a rat HNF3beta expression plasmid resulted in transactivation of only those constructs in which the candidate HNF3 site was present. Furthermore, EMSA using HepG2.1 nuclear extracts containing exogenously expressed HNF3 formed complexes with the same migration and competition properties as those in which the proteins were derived from the differentiated hepatoma cells. Thus, several lines of evidence suggest a critical role for HNF3 in activity from the DHBV S promoter.
Additional file 1 of A validated LC–MS/MS method for analysis of Cabergoline in human plasma with its implementation in a bioequivalent study: investigation of method greenness
Creators: name: Shalaby, Khaled
name: Alghamdi, Saleh
name: Gamal, Mohammed
name: Elhalim, Lobna Mohammed Abd
name: Tony, Rehab Moussa
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Cell Biology
subject: Pharmacology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: Immunology
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Inorganic Chemistry
subject: Science Policy
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Additional file 1: Figure S1. AGREE approach for estimation of new LC/MS/MS method greenness for CAB. Figure S2. AGREE approach for estimation of old LC/MS method greenness for CAB by Allievi and Dostert, in 1998. Figure S3. AGREE approach for estimation of old LC/MS/MS method greenness for CAB by Igarashi et al. in 2003. Table S1. The details for Recovery calculations of Cabergoline. Table S2. The details for Recovery calculations of QUE (IS). Table S3. Matrix effect of Cabergoline. Table S4. Outcomes of stability studies in different environments for the CAB QC samples in human plasma samples. Table S5. Mean Plasma concentrations of CAB versus time after a single dose administration of 2 tablets of test product (0.5 mg Film Coated tablet) and 2 tablets of Dostinex 0.5 mg tablets (reference product).
Additional file 1 of Study protocol for an adapted personal project analysis to measure vertical inter-goal relations on physical activity and diet
Creators: name: Kassas, Sara
name: Culot, Catherine
name: Crombez, Geert
name: Smeding, Annique
name: Leys, Christophe
name: Thompson, Debbe
name: DeSmet, Ann
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences

description: Additional file 1. Description of the Personal Project Analysis Protocol used to collect data.
A hybrid herpesvirus infectious vector based on Epstein-Barr virus and herpes simplex virus type 1 for gene transfer into human cells in vitro and in vivo.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: We have developed a miniviral vector, pH300, based on the human herpesviruses 1 and 4, herpes simplex virus type 1 (HSV-1), and Epstein-Barr virus (EBV), carrying EBV sequences for plasmid episomal maintenance and HSV-1 sequences for amplification and packaging in multimeric form into HSV-1 capsids in the presence of a helper virus and helper cell line. A reporter gene, the bacterial lacZ gene, which expressed beta-galactosidase, was inserted into the multiple cloning site of pH300 to make pH300-lac. The packaged pH300-lac DNA was very efficient in infecting human cells in tissue culture. The pH300-lac miniviral stock was used to infect in vitro various human cell types derived from breast cancer, lung cancer, and liver cancer. Up to 95% of cells were infected and expressed beta-galactosidase activity after exposure to viral stock at a multiplicity of infection of 3. There was essentially no apparent cytotoxicity after infection of cultured cells in vitro. To test in vivo gene delivery, human liver tumor cells preimplanted subcutaneously in nude mice and injected in situ with pH300-lac showed high efficiency of ectopic gene expression. The pH300 miniviral vector is a simple and effective gene transfer system which shows potential for gene therapy of cancer and inherited diseases.
Observations of the Bursting Activity of the 6.7GHz Methanol Maser in G33.641-0.228
Creators: name: Fujisawa, Kenta
name: Aoki, Nozomu
name: Nagadomi, Yoshito
name: Kimura, Saki
name: Shimomura, Tadashi
name: Takase, Genta
name: Sugiyama, Koichiro
name: Motogi, Kazuhito
name: Niinuma, Kotaro
name: Hirota, Tomoya
name: Yonekura, Yoshinori
Publisher: name: arXiv
Year: 2014
Subjects: subject: Solar and Stellar Astrophysics (astro-ph.SR)
subject: FOS: Physical sciences

description: We have observed bursting variability of the 6.7 GHz methanol maser of G33.641-0.228. Five bursts were detected in the observation period of 294 days from 2009 to 2012. The typical burst is a large flux density rise in about one day followed by a slow fall. A non-typical burst observed in 2010 showed a large and rapid flux density enhancement from the stable state, but the rise and fall of the flux density were temporally symmetric and a fast fluctuation continued 12 days. On average, the bursts occurred once every 59 days, although bursting was not periodic. Since the average power required for causing the burst of order of 10^21 Js^-1 is far smaller than the luminosity of G33.641-0.228, a very small fraction of the source's power would be sufficient to cause the burst occasionally. The burst can be explained as a solar-flare like event in which the energy is accumulated in the magnetic field of the circumstellar disk, and is released for a short time. However, the mechanism of the energy release and the dust heating process are still unknown.
description: 11 pages, 4 figures, accepted to PASJ
A viral vaccine vector that expresses foreign genes in lymph nodes and protects against mucosal challenge.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: A candidate live-virus vaccine strain of Venezuelan equine encephalitis virus (VEE) was configured as a replication-competent vector for in vivo expression of heterologous immunogens. Three features of VEE recommend it for use as a vaccine vector. (i) Most human and animal populations are not already immune to VEE, so preexisting immunity to the vector would not limit expression of the heterologous antigen. (ii) VEE replicates first in local lymphoid tissue, a site favoring the induction of an effective immune response. (iii) Parenteral immunization of rodents and humans with live, attenuated VEE vaccines protects against mucosal challenge, suggesting that VEE vaccine vectors might be used successfully to protect against mucosal pathogens. Upon subcutaneous (s.c.) inoculation into the footpad of mice, a VEE vector containing the complete influenza virus hemagglutinin (HA) gene expressed HA in the draining lymph node and induced anti-HA immunoglobulin G (IgG) and IgA serum antibodies, the levels of which could be increased by s.c. booster inoculation. When immunized mice were challenged intranasally with a virulent strain of influenza virus, replication of challenge virus in their lungs was restricted, and they were completely protected from signs of disease. Significant reduction of influenza virus replication in the nasal epithelia of HA vector-immunized mice suggested an effective immunity at the mucosal surface. VEE vaccine vectors represent an alternative vaccination strategy when killed or subunit vaccines are ineffective or when the use of a live attenuated vaccine might be unsafe.
Washington, D.C. and the Growth of Its Early Suburbs : 1860-1920
Creators: name: Levy, Anneli Moucka
Publisher: name: Digital Repository at the University of Maryland
Year: 1980

description: During the nineteenth century, the North American city greatly changed in size and internal structure. With the introduction of mass transportation, large scale suburbanization took place as one aspect of this change. Members of the evolving middle class not only wished to escape the pollution and congestion of the urban core, but also believed strongly in a 'rural ideal,' translated into a 'suburban ideal.' Urban changes and suburban growth were especially pronounced in industrial cities, and descriptions of conditions in these cities identify the accepted model of the spatial configuration of the metropolis existed in 1920. Examination of the growth of Washington D. C. between the Civil War and World War I indicates that the city shared few of the characteristics of the accepted urban model. Nevertheless, it exhibited distinct suburban movement connected with three major transport modes, including the steam railroad. The belief in the 'suburban ideal' was broadly based in Washington and therefore much variation was found among the city's suburban communities, even among those associated with the same transportation mode. Furthermore, in contrast to the suburban model, conditions in the suburban areas often did not compare favorably with those in the city. Even so, the suburbanization process accelerated from small beginnings, so that by 1920 the city displayed the local variant of the typical star-shaped pattern.
Activation of the Epstein-Barr virus DNA polymerase promoter by the BRLF1 immediate-early protein is mediated through USF and E2F.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: The Epstein-Barr virus (EBV) DNA polymerase (pol) is essential for the replication of viral genomes during productive EBV infection. We have previously reported that the EBV DNA pol promoter, which is TATA-less and constitutively inactive, is activated by a genomic clone expressing both immediate-early viral transactivators, BZLF1Z and BRLF1 (R), in EBV-infected lymphoid cells. Here we demonstrate that R alone is sufficient to activate the pol promoter in EBV-negative B cells. Unlike other early promoters to which the R protein binds directly, its effect on the pol promoter does not appear to involve a direct DNA-binding mechanism. Instead, we found that two cellular transcription factors, an upstream stimulatory factor USF, and a member of the E2F family of proteins, bind directly to the pol promoter at positions -795 to -786 and -186 to -170, respectively, regions previously identified as important for activation of the pol promoter. These two sites contribute to or are essential for transactivation of the pol promoter by R in EBV-noninfected B cells. These data suggest that the R immediate-early protein may activate a key early EBV promoter (pol) through both USF and E2F.
Diffusive limit for the random Lorentz gas
Creators: name: Nota, Alessia
Publisher: name: arXiv
Year: 2014
Subjects: subject: Mathematical Physics (math-ph)
subject: FOS: Physical sciences

description: We review some recent results concerning the derivation of the diffusion equation and the validation of Fick's law for the microscopic model given by the random Lorentz Gas. These results are achieved by using a linear kinetic equation as an intermediate level of description between our original mechanical system and the diffusion equation.
description: Proceedings of the conference PSPDE II 2013
Additional file 1 of Reducing malaria transmission in forest-going mobile and migrant populations in Lao PDR and Cambodia: protocol for stepped-wedge cluster-randomised controlled trial
Creators: name: Htike, Win
name: Oo, Win Han
name: Lynn, Thet
name: Sovanda, Lun
name: Agius, Paul A.
name: Oo, May Chan
name: Galau, Naw Hkawng
name: Thu, Kaung Myat
name: Zaw, Aung Khine
name: Htwe, Ei Phyu
name: Cutts, Julia C.
name: Kearney, Ellen A.
name: Scott, Nick
name: O’Flaherty, Katherine
name: Wang, Bangyuan
name: Khamlome, Boualam
name: Vilay, Phoutnalong
name: Siv, Sovannaroth
name: Fowkes, Freya J. I.
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: Biotechnology
subject: Cancer
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: Computational Biology

description: Additional file 1. Supplementary methods for the nested mixed-methods study, quantitative and qualitative data collection tools, and participant information and consent forms.
Alteration of cell cycle kinase complexes in human papillomavirus E6- and E7-expressing fibroblasts precedes neoplastic transformation.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: Expression of viral oncoproteins results in the loss of cell cycle checkpoint control and the accumulation of chromosomal abnormalities. Expression of both human papillomavirus type 16 oncoproteins, E6 and E7, in normal human fibroblasts completely dissociates p21 and proliferating cell nuclear antigen from the quarternary cyclin-cyclin-dependent kinase (CDK) complexes present in normal cells, causes disruption of the cyclin D-CDK4 complex and replacement with a CDK4-p16 complex, and leaves binary complexes of cyclin B1-CDC2 and cyclin A-CDK2 intact. These results are identical to those observed in fully transformed cells. The expression of the individual oncoproteins dramatically affects the association of proliferating cell nuclear antigen into the complexes while leaving the total cellular levels unaltered. Expression of low-risk human papillomavirus has no effect on cyclin complexes. These findings provide evidence for the gross alteration of cyclin-CDK complexes in preneoplastic cells and links this alteration to the loss of genomic stability.
Capsid coding sequence is required for efficient replication of human rhinovirus 14 RNA.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: Mechanisms by which the plus-sense RNA genomes of picornaviruses are replicated remain poorly defined, but existing models do not suggest a role for sequences encoding the capsid proteins. However, candidate RNA replicons (delta P1 beta gal and delta P1Luc), representing the sequence of human rhinovirus 14 virus (HRV-14) with reporter protein sequences (beta-galactosidase or luciferase, respectively) replacing most of the P1 capsid-coding region, failed to replicate in transfected H1-HeLa cells despite efficient primary cleavage of the polyprotein. To determine which P1 sequences might be required for RNA replication, HRV-14 mutants in which segments of the P1 region were removed to frame from the genome were constructed. Mutants with deletions involving the 5'proximal 1,489 nucleotides of the P1 region replicated efficiently, while those with deletions involving the 3' 1,079 nucleotides did not. Reintroduction of the 3' P1 sequence into the nonreplicating delta P1Luc construct resulted in a new candidate replicon, delta P1Luc/VP3, which replicated well and expressed luciferase efficiently. Capsid proteins provided in trans by helper virus failed to rescue the nonreplicating delta P1Luc genome but were able to package the larger-than-genome-length delta P1Luc/VP3 replicon. Thus, a 3'-distal P1 capsid-coding sequence has a previously unrecognized cis-active function related to replication of HRV-14 RNA.
Managing your Private and Public Data: Bringing down Inference Attacks against your Privacy
Creators: name: Salamatian, Salman
name: Zhang, Amy
name: Calmon, Flavio du Pin
name: Bhamidipati, Sandilya
name: Fawaz, Nadia
name: Kveton, Branislav
name: Oliveira, Pedro
name: Taft, Nina
Publisher: name: arXiv
Year: 2014
Subjects: subject: Cryptography and Security (cs.CR)
subject: FOS: Computer and information sciences

description: We propose a practical methodology to protect a user's private data, when he wishes to publicly release data that is correlated with his private data, in the hope of getting some utility. Our approach relies on a general statistical inference framework that captures the privacy threat under inference attacks, given utility constraints. Under this framework, data is distorted before it is released, according to a privacy-preserving probabilistic mapping. This mapping is obtained by solving a convex optimization problem, which minimizes information leakage under a distortion constraint. We address practical challenges encountered when applying this theoretical framework to real world data. On one hand, the design of optimal privacy-preserving mechanisms requires knowledge of the prior distribution linking private data and data to be released, which is often unavailable in practice. On the other hand, the optimization may become untractable and face scalability issues when data assumes values in large size alphabets, or is high dimensional. Our work makes three major contributions. First, we provide bounds on the impact on the privacy-utility tradeoff of a mismatched prior. Second, we show how to reduce the optimization size by introducing a quantization step, and how to generate privacy mappings under quantization. Third, we evaluate our method on three datasets, including a new dataset that we collected, showing correlations between political convictions and TV viewing habits. We demonstrate that good privacy properties can be achieved with limited distortion so as not to undermine the original purpose of the publicly released data, e.g. recommendations.
Protecting a solid-state spin from decoherence using dressed spin states
Creators: name: Golter, D. Andrew
name: Baldwin, Thomas K.
name: Wang, Hailin
Publisher: name: arXiv
Year: 2014
Subjects: subject: Quantum Physics (quant-ph)
subject: Mesoscale and Nanoscale Physics (cond-mat.mes-hall)
subject: FOS: Physical sciences

description: Dressed spin states, a spin coupling to continuous radiation fields, can fundamentally change how a spin responds to magnetic fluctuations. Using dressed spin states, we were able to protect an electron spin in diamond from decoherence. Dressing a spin with resonant microwaves at a coupling rate near 1 MHz leads to a 50 times reduction in the linewidth of the spin transition, limited by transit-time broadening. The spin decoherence and the energy level structure of the dressed states were probed with optical coherent-population-trapping processes. Compared with dynamical decoupling, where effects of the bath are averaged out at specific times, the dressed state provides a continuous protection from decoherence.
Conditional human immunodeficiency virus type 1 protease mutants show no role for the viral protease early in virus replication.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: The human immunodeficiency virus type 1 protease plays a critical role in the proteolytic processing of precursor polyproteins during virion maturation. Contradictory evidence has been obtained for a possible role for the protease early after infection, i.e., during DNA synthesis and/or integration. We have reexamined this question by using conditional mutants of the protease. In one set of experiments, protease mutants that confer a temperature-sensitive phenotype for processing were used to assess the need for protease activity early after infection. No significant difference from results with wild-type virus was seen when infections were carried out at either 35 or 40 degrees C. In a separate set of experiments, infections were carried out in the presence of a protease inhibitor. In this case, both wild-type virus and a drug-resistant variant were used, the latter as a control to ensure a specific effect of the inhibitor. Infection with either virus was not inhibited at drug concentrations that were up to 10-fold higher than those needed to inhibit intracellular processing by the viral protease. The results obtained by both of these experimental protocols provide evidence that the human immunodeficiency virus type 1 protease does not play a role early after infection.
Additional file 1 of Qualitative evaluation of postdoctoral trainee and faculty advisor experiences within a research-intensive school of pharmacy
Creators: name: McLaughlin, JE
name: Morbitzer, KA
name: Hahn, F
name: Minshew, L
name: Brouwer, KLR
Publisher: name: figshare
Year: 2022
Subjects: subject: Medicine
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences
subject: Science Policy

description: Additional file 1: Appendix 1.
Solvable non-Hermitian discrete square well with closed-form physical inner product
Creators: name: Znojil, Miloslav
Publisher: name: arXiv
Year: 2014
Subjects: subject: Mathematical Physics (math-ph)
subject: Quantum Physics (quant-ph)
subject: FOS: Physical sciences

description: A non-Hermitian $N-$level quantum model with two free real parameters is proposed in which the bound-state energies are given as roots of an elementary trigonometric expression and in which they are, in a physical domain of parameters, all real. The wave function components are expressed as closed-form superpositions of two Chebyshev polynomials. In any eligible physical Hilbert space of finite dimension $N < \infty$ our model is constructed as unitary with respect to an underlying Hilbert-space metric $Θ\neq I$. The simplest version of the latter metric is finally constructed, at any dimension $N=2,3,\ldots$, in closed form. This version of the model may be perceived as an exactly solvable $N-$site lattice analogue of the $N=\infty$ square well with complex Robin-type boundary conditions. At any $N
description: 23 pp., 8 figures