cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Epidemiology of ticks submitted from human hosts in Alberta, Canada (2000–2019)
Creators: name: Kanji, Jamil N.
name: Isaac, Abraam
name: Gregson, Daniel
name: Mierzejewski, Monika
name: Shpeley, Danny
name: Tomlin, Pauline
name: Groeschel, Michael
name: Lindsay, L. Robbin
name: Lachance, Lisa
name: Kowalewska-Grochowska, Kinga
Publisher: name: Taylor & Francis
Year: 2022
Subjects: subject: Microbiology
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Environmental Sciences not elsewhere classified
subject: Ecology
subject: Biological Sciences not elsewhere classified
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Virology
subject: Computational Biology

description: The geographic range and occurrence of tick species is dynamic. This has important public health implications due to important tick species that can transmit pathogens. This study presents a retrospective review of tick genera recovered from humans and submitted for identification in Alberta, Canada, over a 19-year period. The total number of ticks and proportion of genera were analyzed over time. Molecular testing for a number of pathogens associated with Ixodes scapularis and I. pacificus was conducted. A total of 2,358 ticks were submitted between 2000 and 2019, with 98.6% being acquired in Alberta. The number of ticks submitted increased significantly over time (p < 0.0001). Dermacentor ticks were the most abundant genus, followed by Ixodes and Amblyomma. There was a significant decrease in the proportion of Dermacentor ticks between 2013 and 2019 (p = 0.02), with a corresponding increase in the proportion of Ixodes ticks over the same time (p = 0.04). No statistically significant change in seasonality was identified. Borrelia burgdorferi was detected in 8/76 (10.5%; 95% CI 5.4–19.4%) of all I. scapularis and I. pacificus ticks submitted. This translated to a B. burgdorferi positivity of 0.35% (95% CI 0.15–0.68%) among all ticks received. Dermacentor species (especially D. andersoni) remains the most common tick feeding on humans in Alberta. Small numbers of vector species (including I. scapularis/pacificus) are encountered annually over widely separated geographic areas in the province. The risk of exposure to tick-borne pathogens (e.g. Lyme disease) in Alberta remains low.
Polyeuktos, Eupsychios, Clement, Primus, Lollius and Dabsos, martyrs of Nicomedia
Creators: name: Admin, CSLA
Publisher: name: University of Oxford
Year: 2016
Subjects: History

description: All evidence related to Polyeuktos, Eupsychios, Clement, Primus, Lollius and Dabsos, martyrs of Nicomedia.
Genotype-by-environment (temperature-humidity) interaction of milk production traits in Australian Holstein cattle
Creators: name: Cheruiyot, Evans Kiptoo
name: Nguyen, TTT
name: Haile-Mariam, Mekonnen
name: Cocks, Benjamin
name: Abdelsayed, Mary
name: Pryce, Jennie
Publisher: name: La Trobe
Year: 2023
Subjects: classificationCode: 3003
subject: Animal production

description: © 2020 American Dairy Science Association Dairying in Australia is practiced in highly diverse climatic conditions and production systems, which means that re-ranking of genotypes could occur across environments that vary in temperature and humidity—that is, genotype-by-environment interactions (G × E) may exist. The objective of this study was to investigate G × E for heat tolerance with respect to milk production traits in Australian Holsteins. A total of 6.7 million test-day milk yield records for first, second, and third lactations from 491,562 cows and 6,410 sires that had progeny in different climatic environments were included in the analysis. The environmental gradient used was the temperature-humidity index (THI) calculated from climate data from 163 Australian public weather stations between 2003 and 2017. Data were analyzed using univariate reaction norm (RM) sire model, and the results were compared with multi-trait model (MT). The MT analysis treated test-day yields at 5th percentile (THI = 61; i.e., thermoneutral conditions), 50th percentile (THI = 67; i.e., moderate heat stress conditions), and 95th percentile (THI = 73; i.e., high heat stress conditions) of the trajectory of THI as correlated traits. A THI series of 61, 67, and 73, for example, is equivalent to average temperature and relative humidity of approximately 20°C and 45%, 25°C and 45%, and 31°C and 50%, respectively. We observed some degree of heterogeneity of additive (AG) and permanent environmental (PE) variance over the trajectory THI from RM analysis, with estimates decreasing at higher THI values more steeply for PE than for AG variance. The genetic correlations of the tests between the 5th and 95th percentiles of THI for milk, protein, and fat yield from RM were 0.88 ± 0.01 (standard error), 0.79 ± 0.01, and 0.86 ± 0.01, respectively, whereas the corresponding estimates from MT were 0.86 ± 0.02, 0.84 ± 0.03, and 0.87 ± 0.03. We observed lower genetic correlations between the 5th and 95th percentiles of THI for milk tests from recent years (i.e., 2009 and 2017) compared with earlier years (i.e., 2003 and 2008), which suggests that the level of G × E is increasing in the studied population and should be monitored especially in anticipation of future expected increase in daily average temperature and frequency of heat events. Overall, our results indicate presence of G × E at the upper extreme of the trajectory of THI, but the current extent of sire re-ranking may not justify providing separate genetic evaluations for different levels of heat stress. However, variations observed in the sire sensitivity to heat stress suggest that dairy herds in high heat load conditions could benefit more from using heat-tolerant or resilient sires.
Genotype-by-environment (temperature-humidity) interaction of milk production traits in Australian Holstein cattle
Creators: name: Evans Kiptoo Cheruiyot
name: TTT Nguyen
name: M Haile-Mariam
name: Cocks, Benjamin
name: Abdelsayed, Mary
name: Pryce, Jennie
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: © 2020 American Dairy Science Association Dairying in Australia is practiced in highly diverse climatic conditions and production systems, which means that re-ranking of genotypes could occur across environments that vary in temperature and humidity—that is, genotype-by-environment interactions (G × E) may exist. The objective of this study was to investigate G × E for heat tolerance with respect to milk production traits in Australian Holsteins. A total of 6.7 million test-day milk yield records for first, second, and third lactations from 491,562 cows and 6,410 sires that had progeny in different climatic environments were included in the analysis. The environmental gradient used was the temperature-humidity index (THI) calculated from climate data from 163 Australian public weather stations between 2003 and 2017. Data were analyzed using univariate reaction norm (RM) sire model, and the results were compared with multi-trait model (MT). The MT analysis treated test-day yields at 5th percentile (THI = 61; i.e., thermoneutral conditions), 50th percentile (THI = 67; i.e., moderate heat stress conditions), and 95th percentile (THI = 73; i.e., high heat stress conditions) of the trajectory of THI as correlated traits. A THI series of 61, 67, and 73, for example, is equivalent to average temperature and relative humidity of approximately 20°C and 45%, 25°C and 45%, and 31°C and 50%, respectively. We observed some degree of heterogeneity of additive (AG) and permanent environmental (PE) variance over the trajectory THI from RM analysis, with estimates decreasing at higher THI values more steeply for PE than for AG variance. The genetic correlations of the tests between the 5th and 95th percentiles of THI for milk, protein, and fat yield from RM were 0.88 ± 0.01 (standard error), 0.79 ± 0.01, and 0.86 ± 0.01, respectively, whereas the corresponding estimates from MT were 0.86 ± 0.02, 0.84 ± 0.03, and 0.87 ± 0.03. We observed lower genetic correlations between the 5th and 95th percentiles of THI for milk tests from recent years (i.e., 2009 and 2017) compared with earlier years (i.e., 2003 and 2008), which suggests that the level of G × E is increasing in the studied population and should be monitored especially in anticipation of future expected increase in daily average temperature and frequency of heat events. Overall, our results indicate presence of G × E at the upper extreme of the trajectory of THI, but the current extent of sire re-ranking may not justify providing separate genetic evaluations for different levels of heat stress. However, variations observed in the sire sensitivity to heat stress suggest that dairy herds in high heat load conditions could benefit more from using heat-tolerant or resilient sires.
Supplementary_Figures_1-5_Gu_et_al. – Supplemental material for Vesicle Transport in Plants: A Revised Phylogeny of SNARE Proteins
Creators: name: Xiaoyan Gu
name: Brennan, Adrian
name: Wenbin Wei
name: Guangqin Guo
name: Lindsey, Keith
Publisher: name: SAGE Journals
Year: 2020
Subjects: Cell Biology

description: Supplemental material, Supplementary_Figures_1-5_Gu_et_al. for Vesicle Transport in Plants: A Revised Phylogeny of SNARE Proteins by Xiaoyan Gu, Adrian Brennan, Wenbin Wei, Guangqin Guo and Keith Lindsey in Evolutionary Bioinformatics
Aboriginal people with chronic HCV: The role of community health nurses for improving health-related quality of life
Creators: name: A Rashidi
name: Higgs, Peter
name: S Carruthers
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: © 2019 Australian College of Nursing Ltd Introduction: There is a lack of knowledge about Health-Related Quality of Life (HRQL) of Aboriginal Australians with self-reported hepatitis C infection in Western Australia. This marginalised group of people is disproportionally affected by the hepatitis C virus (HCV) for which there is no preventative vaccine. This study provides data that help understand the long-term consequences of living with HCV infection within the Aboriginal community. It outlines opportunities for nursing interventions for hepatitis care that focus on supporting mental health and drug and alcohol issues within this population. Methods: We surveyed 123 Aboriginal people living with HCV in a community setting. Survey data included demographics, drug use history, length of time since diagnosis, changes in lifestyle since diagnosis, fatigue, social support, alcohol consumption, and physical and mental health measures. Results: Most participants reported amphetamine injecting frequently in the last six months and their duration of injecting drug ranged from 8 to more than 11 years. Additionally, half of the participants were classified as high-risk alcohol users. Overall 52% of participants were in poor physical and 60% of participants were in poor mental health. Discussion: This survey of Aboriginal people with self-reported hepatitis C infection indicates substantial problems of mental and physical comorbidities among this population. Conclusion: There is a need for the development of HCV community clinics in Aboriginal health care settings with trained Aboriginal community health nurses to reduce problematic alcohol consumption, assess liver health and subsequently provide HCV treatment in a culturally appropriate way.
Different impact of bovine complement regulatory protein 46 (CD46bov) as a cellular receptor for members of the species Pestivirus H and Pestivirus G
Creators: name: Leveringhaus, Elena
name: Cagatay, Gökce Nur
name: Hardt, Juliane
name: Becher, Paul
name: Postel, Alexander
Publisher: name: Taylor & Francis
Year: 2022
Subjects: subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Environmental Sciences not elsewhere classified
subject: Ecology
subject: Biological Sciences not elsewhere classified
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Virology

description: The genus Pestivirus within the family Flaviviridae comprises highly relevant animal pathogens such as bovine viral diarrhoea virus 1 and 2 (BVDV-1 and -2) classified into the two species Pestivirus A and Pestivirus B, respectively. First described in 2004, HoBi-like pestiviruses (HoBiPeV) represent emerging bovine pathogens that belong to a separate species (Pestivirus H), but share many similarities with BVDV-1 and -2. Additionally, two giraffe pestivirus (GPeV) strains both originating from Kenya represent another distinct species (Pestivirus G), whose members replicate very efficiently in bovine cells. In this study, we investigated the role of bovine complement regulatory protein 46 (CD46bov), the receptor of BVDV-1 and -2, in the entry of HoBiPeV and GPeV. For this purpose, bovine CD46-knockout and CD46-rescue cell lines were generated by CRISPR/Cas9 technology and subsequent trans-complementation, respectively. Our results provide strong evidence that the impact of CD46bov differs between viruses belonging to Pestivirus H and viruses representing Pestivirus G: CD46bov revealed to be a major cellular entry factor for HoBiPeV strain HaVi-20. In contrast, GPeV strain PG-2 presented as largely independent of CD46bov, suggesting a different entry mechanism involving other molecular determinants which remain to be identified. In addition, we demonstrated that, similar to BVDV-1 and -2, virus isolates of both Pestivirus H and Pestivirus G are able to adapt to cell culture conditions by using heparan sulfate to enter the host cell. In conclusion, our findings show that different bovine pestiviruses use diverse mechanisms of host cell entry.
Andersen_FigS1 – Supplemental material for Playing With Fear: A Field Study in Recreational Horror
Creators: name: Andersen, Marc Malmdorf
name: Schjoedt, Uffe
name: Price, Henry
name: Rosas, Fernando E.
name: Coltan Scrivner
name: Clasen, Mathias
Publisher: name: SAGE Journals
Year: 2020
Subjects: subject: 110904 Neurology and Neuromuscular Diseases
subject: FOS: Clinical medicine
subject: 170199 Psychology not elsewhere classified
subject: FOS: Psychology
subject: 110319 Psychiatry (incl. Psychotherapy)
subject: Neuroscience

description: Supplemental material, Andersen_FigS1 for Playing With Fear: A Field Study in Recreational Horror by Marc Malmdorf Andersen, Uffe Schjoedt, Henry Price, Fernando Rosas, Coltan Scrivner and Mathias Clasen in Psychological Science
Different impact of bovine complement regulatory protein 46 (CD46bov) as a cellular receptor for members of the species Pestivirus H and Pestivirus G
Creators: name: Leveringhaus, Elena
name: Cagatay, Gökce Nur
name: Hardt, Juliane
name: Becher, Paul
name: Postel, Alexander
Publisher: name: Taylor & Francis
Year: 2021
Subjects: subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Environmental Sciences not elsewhere classified
subject: Ecology
subject: Biological Sciences not elsewhere classified
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Virology

description: The genus Pestivirus within the family Flaviviridae comprises highly relevant animal pathogens such as bovine viral diarrhoea virus 1 and 2 (BVDV-1 and -2) classified into the two species Pestivirus A and Pestivirus B, respectively. First described in 2004, HoBi-like pestiviruses (HoBiPeV) represent emerging bovine pathogens that belong to a separate species (Pestivirus H), but share many similarities with BVDV-1 and -2. Additionally, two giraffe pestivirus (GPeV) strains both originating from Kenya represent another distinct species (Pestivirus G), whose members replicate very efficiently in bovine cells. In this study, we investigated the role of bovine complement regulatory protein 46 (CD46bov), the receptor of BVDV-1 and -2, in the entry of HoBiPeV and GPeV. For this purpose, bovine CD46-knockout and CD46-rescue cell lines were generated by CRISPR/Cas9 technology and subsequent trans-complementation, respectively. Our results provide strong evidence that the impact of CD46bov differs between viruses belonging to Pestivirus H and viruses representing Pestivirus G: CD46bov revealed to be a major cellular entry factor for HoBiPeV strain HaVi-20. In contrast, GPeV strain PG-2 presented as largely independent of CD46bov, suggesting a different entry mechanism involving other molecular determinants which remain to be identified. In addition, we demonstrated that, similar to BVDV-1 and -2, virus isolates of both Pestivirus H and Pestivirus G are able to adapt to cell culture conditions by using heparan sulfate to enter the host cell. In conclusion, our findings show that different bovine pestiviruses use diverse mechanisms of host cell entry.
Hyponatremia
Creators: name: HAQUE, MUHAMMAD TANZEEL UL
Publisher: name: Zenodo
Year: 2024
Subjects: Sodium
SIADH
Osmolality
Osmotic demyleination
Hypervolemia
hyponatraemia

description: Hyponatremia is a significant finding, a clinician should know how to interpret it. This presentation includes relevant scenarios and types of low levels of sodium.  There are foot notes in certain slides to provide more insight. 

This presentaion was delivered on 13-Aug-2024 at County Hospital, Stafford, UK.
The nature and extent of upper limb associated reactions during walking in people with acquired brain injury
Creators: name: Kahn, Michelle B
name: Clark, Ross A
name: Williams, Gavin
name: Bower, Kelly J
name: Banky, Megan
name: Olver, John
name: Mentiplay, Benjamin
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: © 2019 The Author(s). Background: Upper limb associated reactions (ARs) are common in people with acquired brain injury (ABI). Despite this, there is no gold-standard outcome measure and no kinematic description of this movement disorder. The aim of this study was to determine the upper limb kinematic variables most frequently affected by ARs in people with ABI compared with a healthy cohort at matched walking speed intention. Methods: A convenience sample of 36 healthy control adults (HCs) and 42 people with ABI who had upper limb ARs during walking were recruited and underwent assessment of their self-selected walking speed using the criterion-reference three dimensional motion analysis (3DMA) at Epworth Hospital, Melbourne. Shoulder flexion, abduction and rotation, elbow flexion, forearm rotation and wrist flexion were assessed. The mean angle, standard deviation (SD), peak joint angles and total joint angle range of motion (ROM) were calculated for each axis across the gait cycle. On a group level, ANCOVA was used to assess the between-group differences for each upper limb kinematic outcome variable. To quantify abnormality prevalence on an individual participant level, the percentage of ABI participants that were outside of the 95% confidence interval of the HC sample for each variable were calculated. Results: There were significant between-group differences for all elbow and shoulder abduction outcome variables (p < 0.01), most shoulder flexion variables (except for shoulder extension peak), forearm rotation SD and ROM and for wrist flexion ROM. Elbow flexion and shoulder abduction were the axes most frequently affected by ARs. Despite the elbow being the most prevalently affected (38/42, 90%), a large proportion of participants had abnormality, defined as ±1.96 SD of the HC mean, present at the shoulder (32/42, 76%), forearm (20/42, 48%) and wrist joints (10/42, 24%). Conclusion: This study provides valuable information on ARs, and highlights the need for clinical assessment of ARs to include all of the major joints of the upper limb. This may inform the development of a criterion-reference outcome measure or classification system specific to ARs.
Understanding the factors that influence the quality of paid disability support for adults with acquired neurological disability and complex needs: a scoping review protocol
Creators: name: Topping, Megan Ciara
name: Douglas, Jacinta
name: Winkler, Dianne
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: Introduction Adults with disability as the result of an acquired neurological disorder often have varying and complex support needs. Consequently, adults with complex needs often require paid support to enable them to exercise choice and control and participate fully and effectively in society. Given support is so critical for this population, this scoping review aims to further understand the factors that influence the quality of paid disability support for adults with acquired neurological disability and complex needs. Methods and analysis The methodology proposed by Arksey and O'Malley, with the modifications recommended by Levac and O'Brien and the Preferred Reporting Items for Systematic Reviews and Meta-Analysis: extension for Scoping Reviews will be used. Systematic and comprehensive electronic database searches will be conducted in MEDLINE, CINAHL, PsycINFO, Scopus and Embase. The review will follow a six-stage framework including (1) identifying the research question/s, (2) identifying the relevant studies, with (3) study selection and (4) charting of data by two independent coders, (5) collating, summarising and reporting data and (6) expert consultation which will be sought from people with lived experience, as well as clinical and academic experts. Work on this scoping review began in June 2019 and will be completed by June 2020. Ethics and dissemination Ethical approval will not be required to conduct the scoping review. However, the consultation with people with lived experience will be conducted via codesign workshops. The codesign workshops have received ethical approval from La Trobe University Ethics Committee (reference number: HEC19232). It is intended that review findings will be made available to relevant stakeholders through a peer-reviewed publication, conference presentations and workshops.
Grain crushing in geoscience materials – Key issues on crushing response, measurement and modeling: Review and preface
Creators: name: Y Xiao
name: CS Desai
name: A Daouadji
name: AW Stuedlein
name: H Liu
name: Naga, Hossam Aboel
Publisher: name: La Trobe
Year: 2020
Subjects: Uncategorized

description: © 2020 China University of Geosciences (Beijing) and Peking University Grain crushing is commonly encountered in deep foundation engineering, high rockfill dam engineering, railway engineering, mining engineering, coastal engineering, petroleum engineering, and other geoscience application. Grain crushing is affected by fundamental soil characteristics, such as their mineral strength, grain size and distribution, grain shape, density and specimen size, and also by external factors including stress magnitude and path, loading rate and duration, degree of saturation, temperature and geochemical environment. Crushable material becomes a series of different materials with the change in its grading during grain crushing, resulting in a decrease in strength and dilatancy and an increase in compressibility. Effects of grain crushing on strength, dilatancy, deformation and failure mechanisms have been extensively investigated through laboratory testing, discrete element method (DEM) modelling, Weibull statistics, and constitutive modelling within the framework of the extended crushing-dependent critical state theory or the energy-based theory. Eleven papers summarized in this review article for this special issue addressed the above issues in grain crushing through the advanced testing and modelling.
Additional file 7 of Genome wide transcriptome analysis provides bases on hepatic lipid metabolism disorder affected by increased dietary grain ratio in fattening lambs
Creators: name: Mi, Hui
name: Hu, Fan
name: Gebeyew, Kefyalew
name: Cheng, Yan
name: Du, Ruiping
name: Gao, Min
name: He, Zhixiong
name: Tan, Zhiliang
Publisher: name: figshare
Year: 2024
Subjects: subject: Biochemistry
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Physiology
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: Biological Sciences not elsewhere classified
subject: Computational Biology

description: Fig.S1 GO enrichment analysis of DEGs between GN60 and GN70. Fig.S2 Protein?protein interaction (PPI) network analysis. (A) PPI network for DEGs. The Top3 highly connected clusters through MCL clustering, including (B) DNA replication, (C) Fatty acid degradation, (D) Cholesterol metabolism.
Raising an Assistance Dog Puppy—Stakeholder Perspectives on What Helps and What Hinders
Creators: name: Mai, Dac Loc
name: Howell, Tiffani
name: Pree Benton
name: Bennett, Pauleen
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: © 2020 by the authors. Assistance dog puppies live with their raisers for up to 16 months before entering advanced training and, hopefully, becoming qualified to help people with a disability. Almost half of the puppies fail to meet the behavioural standards required for assistance dogs, and some puppy raisers produce more behaviourally favourable puppies than others. It is unclear what factors influence puppy-raising practice quality. To understand this, we interviewed 17 participants, including experienced puppy raisers (n = 8), provider organisation staff (n = 4), and those who have served both as puppy raisers and staff (n = 5). Results of a thematic analysis suggest three groups of influencing factors, namely intrapersonal factors, social support, and puppy characteristics. Intrapersonal factors such as expectations, competency, perseverance and passion were reported to influence puppy raisers’ experiences, puppy-raising quality, and continuity of service. Contextual factors such as availability of social support (informational and emotional supports) and less-demanding puppies both led to positive puppy-raising experiences, while the former also contributed to puppy raisers’ perceptions of competency. Future research should quantitatively examine the interrelationships of these factors concerning puppies’ behavioural development. Meanwhile, organisations could consider these factors when developing their recruitment and puppy-raiser support programs.
Human hepatocyte-enriched miRNA-192-3p promotes HBV replication through inhibiting Akt/mTOR signalling by targeting ZNF143 in hepatic cell lines
Creators: name: Li, Fahong
name: Deng, Yingying
name: Zhang, Shenyan
name: Zhu, Beidi
name: Wang, Jun
name: Wang, Jinyu
name: Wang, Xueyu
name: Zhao, Zhenyu
name: Deng, Wanyu
name: Mao, Richeng
name: Shen, Zhongliang
name: Chen, Jieliang
name: Broering, Ruth
name: Lin, Yong
name: Lu, Mengji
name: Zhang, Jiming
Publisher: name: Taylor & Francis
Year: 2022
Subjects: subject: Biophysics
subject: Biochemistry
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Physiology
subject: Pharmacology
subject: Chemical Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Cancer
subject: Hematology
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Virology

description: Previous studies have revealed multiple tissue- or cell-specific or enriched miRNA profiles. However, miRNA profiles enriched in hepatic cell types and their effect on HBV replication have not been well elucidated. In this study, primary human hepatocytes (PHHs), Kupffer cells (KCs), liver sinusoidal endothelial cells (LSECs), and hepatic stellate cells (HSCs) were prepared from liver specimens of non-HBV-infected patients. Four hepatic cell type-enriched miRNA profiles were identified from purified liver cells miRNA microarray assay. The results revealed that 12 miRNAs, including miR-122-5p, miR-122-3p, miR-192-5p and miR-192-3p were PHH-enriched; 9 miRNAs, including miR-142-5p and miR-155-5p were KC-enriched; 6 miRNAs, including miR-126-3p and miR-222-3p were LSEC-enriched; and 14 miRNAs, including miR-214-3p and miR-199a-3p were HSC-enriched. By testing the effect of 11 PHH-enriched miRNAs on HBV production, we observed that miR-192-3p had the greatest pro-virus effect in hepatic cell lines, HepG2.2.15 and Huh7 cells transfected with an HBV replicon plasmid. Moreover, we further found that HmiR-192-3p promoted HBV replication and gene expression through inhibiting Akt/mTOR signalling by direct targeting of ZNF143 in HepG2.2.15 cells. Additionally, the serum and hepatic miR-192-3p expression levels was significantly higher in chronic hepatitis B patients than in healthy controls and serum miR-192-3p positively correlated with the serum levels of HBV DNA and HBsAg. In summary, we identified miRNA profiles enriched in four hepatic cell types and revealed that PHHs-enriched miR-192-3p promoted HBV replication through inhibiting Akt/mTOR signalling by direct targeting of ZNF143 in hepatic cell lines. Our study provides specific perspective for the role of hepatic cell type-enriched miRNA in interaction with viral replication and various liver pathogenesis.
Additional file 1 of Development and validation of a nomogram for predicting metabolic-associated fatty liver disease in the Chinese physical examination population
Creators: name: Zhou, Bingqian
name: Gong, Ni
name: Huang, Xinjuan
name: Zhu, Jingchi
name: Qin, Chunxiang
name: He, Qingnan
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Sociology
subject: FOS: Sociology
subject: Biological Sciences not elsewhere classified
subject: Mathematical Sciences not elsewhere classified

description: Additional file 1: Supplementary Fig. 1. Subject selection flowchart. Supplementary Fig. 2. ROC curves for the prediction model: (a) the development dataset and (b) the validation dataset. Supplementary Fig. 3. Calibration curves for the nomogram for predicting MAFLD risk (a) the development dataset and (b) the validation dataset. Note: The black line indicates the perfect prediction of the ideal model and the dotted line indicates the performance of the nomogram. Calibration curves that are closer to the diagonal line have higher prediction accuracy. Supplementary Table 1. Predictors of MAFLD risk in the physical examination population.
Replicative transposition contributes to the evolution and dissemination of KPC-2-producing plasmid in Enterobacterales
Creators: name: Tang, Yu
name: Li, Gang
name: Shen, Pinghua
name: Zhang, Ying
name: Jiang, Xiaofei
Publisher: name: Taylor & Francis
Year: 2021
Subjects: subject: Microbiology
subject: FOS: Biological sciences
subject: Genetics
subject: Biotechnology
subject: Evolutionary Biology
subject: Environmental Sciences not elsewhere classified
subject: Ecology
subject: Biological Sciences not elsewhere classified
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Virology

description: Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacterales are prevalent worldwide and pose an alarming threat to public health. The incidence and transmission of blaKPC-2 gene via horizontal gene transfer (e.g., transposition) have been well documented. However, the dynamics of transposon structure bearing blaKPC-2 and their exact effects on the evolution and dissemination of blaKPC-2 gene are not well characterized. Here, we collected all 161 carbapenem-resistant Enterobacterales (CRE) isolates during the early stage of CRE pandemic. We observed that the prevalence of KPC-2-producing Enterobacterales was mediated by multiple species and sequence types (STs), and that blaKPC-2 gene was located on three diverse variants of Tn1721 in multi-drug resistance (MDR) region of plasmid. Notably, outbreak of KPC-2-producing plasmid is correlated with the dynamics of transposon structure. Furthermore, we experimentally demonstrated that replicative transposition of Tn1721 and IS26 promotes horizontal transfer of blaKPC-2 and evolution of KPC-2-producing plasmid. The Tn1721 variants appearing concurrently with the peak of epidemic (A2- and B-type) showed higher transposition frequencies and certain superior ability to propagation. Overall, our work suggests replicative transposition contributes to the evolution and transmission of KPC-2-producing plasmid and highlights its important role in the inter- and intra-species dissemination of blaKPC-2 gene in Enterobacterales.
A new testing platform using fingerstick blood for quantitative antibody response evaluation after SARS-CoV-2 vaccination
Creators: name: Du, Jinwei
name: Zhang, Dayu
name: Pathakamuri, Joseph A.
name: Kuebler, Daniel
name: Yang, Ying
name: Loginova, Yulia
name: Chu, Eric
name: Madej, Roberta
name: Neves, Jocelyn V.
name: Singer, Brianna
name: Radke, Holly
name: Spencer, Naomi
name: Rizk, Elizabeth
name: Zhang, Aiguo
name: Lu, Chuanyi M.
name: Sha, Michael Y.
Publisher: name: Taylor & Francis
Year: 2022
Subjects: subject: Biochemistry
subject: Molecular Biology
subject: Physiology
subject: FOS: Biological sciences
subject: Biotechnology
subject: Immunology
subject: FOS: Clinical medicine
subject: Cancer
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Virology
subject: Cardiology

description: Testing and vaccination have been major components of the strategy for combating the ongoing COVID-19 pandemic. In this study, we have developed a quantitative anti-SARS-CoV-2 spike (S1) IgG antibody assay using a fingerstick dried blood sample. We evaluated the feasibility of using this high-throughput and quantitative anti-SARS-CoV-2 spike (S1) IgG antibody testing assay in vaccinated individuals. Fingerstick blood samples were collected and analyzed from 137 volunteers before and after receiving the Moderna or Pfizer mRNA vaccine. Anti-SARS-CoV-2 S1 IgG antibody could not be detected within the first 7 days after receiving the first vaccine dose, however, the assay reliably detected antibodies from day 14 onwards. In addition, no anti-SARS-CoV-2 nucleocapsid (N) protein IgG antibody was detected in any of the vaccinated or healthy participants, indicating that the anti-SARS-CoV-2 S1 IgG assay is specific for the mRNA vaccine-induced antibodies. The S1 IgG levels detected in fingerstick samples correlated with the levels found in venous blood plasma samples and with the efficacy of venous blood plasma samples in the plaque reduction neutralization test (PRNT). The assay displayed a limit of quantification (LOQ) of 0.59 μg/mL and was found to be linear in the range of 0.51-1000 μg/mL. Finally, its clinical performance displayed a Positive Percent Agreement (PPA) of 100% (95% CI: 0.89-1.00) and a Negative Percent Agreement (NPA) of 100% (95% CI: 0.93-1.00). In summary, the assay described here represents a sensitive, precise, accurate, and simple method for the quantitative detection and monitoring of post-vaccination anti-SARS-CoV-2 spike IgG responses.
Additional file 1 of Genome wide transcriptome analysis provides bases on hepatic lipid metabolism disorder affected by increased dietary grain ratio in fattening lambs
Creators: name: Mi, Hui
name: Hu, Fan
name: Gebeyew, Kefyalew
name: Cheng, Yan
name: Du, Ruiping
name: Gao, Min
name: He, Zhixiong
name: Tan, Zhiliang
Publisher: name: figshare
Year: 2024
Subjects: subject: Biochemistry
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Physiology
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: Biological Sciences not elsewhere classified
subject: Computational Biology

description: Table S1. Ingredient and chemical composition of diet fed during the experiment (DM basis)
‘We are not just a union, we are a family’ class, kinship and tribe in Zambia’s mining unions
Creators: name: Kapesea, Robby
name: McNamara, Thomas
Publisher: name: La Trobe
Year: 2020
Subjects: Uncategorized

description: © 2020, The Author(s). Trade unions in Zambia and in several other developing countries have been understood to create ‘detribalising’ class consciousnesses. In contrast, we argue that Zambian understandings of unionism have developed through similar political economic processes to those that generated ‘tribes’. Values and structures that enable concepts of the good life more commonly found among Bemba speakers and Eastern Zambians have been naturalised into Zambia’s mining unions, guiding union policy and practice in a manner which limits North Western Zambians’ union participation. Utilising Lazar's (2018) understanding of unionism as kinship, we explore how Zambians of various tribes attempt to utilise unions to achieve what they see as human flourishing and social justice. We foreground that people’s understandings of the good life frequently incorporate gendered and gerontocratic hierarchies and we demonstrate that intra-national unionisms are co-created through (and influence) local cultural norms and political histories. This encourages anthropologists of trade unionism to ask what values and hierarchies are rendered invisible in other union ‘families’, and to explore intertwinements between unions and communities enabled through kinship, rather than through Civil Society Organisations.
Genomic Epidemiology in Filarial Nematodes: Transforming the Basis for Elimination Program Decisions
Creators: name: Hedtke, Shannon
name: Kuesel, AC
name: Crawford, Katie
name: Graves, PM
name: Boussinesq, M
name: Lau, CL
name: Boakye, DA
name: Grant, Warwick
Publisher: name: La Trobe
Year: 2022
Subjects: classificationCode: 31
subject: Biological sciences
subject: FOS: Biological sciences
classificationCode: 3105
subject: Genetics

description: Onchocerciasis and lymphatic filariasis are targeted for elimination, primarily using mass drug administration at the country and community levels. Elimination of transmission is the onchocerciasis target and global elimination as a public health problem is the end point for lymphatic filariasis. Where program duration, treatment coverage, and compliance are sufficiently high, elimination is achievable for both parasites within defined geographic areas. However, transmission has re-emerged after apparent elimination in some areas, and in others has continued despite years of mass drug treatment. A critical question is whether this re-emergence and/or persistence of transmission is due to persistence of local parasites—i.e., the result of insufficient duration or drug coverage, poor parasite response to the drugs, or inadequate methods of assessment and/or criteria for determining when to stop treatment—or due to re-introduction of parasites via human or vector movement from another endemic area. We review recent genetics-based research exploring these questions in Onchocerca volvulus, the filarial nematode that causes onchocerciasis, and Wuchereria bancrofti, the major pathogen for lymphatic filariasis. We focus in particular on the combination of genomic epidemiology and genome-wide associations to delineate transmission zones and distinguish between local and introduced parasites as the source of resurgence or continuing transmission, and to identify genetic markers associated with parasite response to chemotherapy. Our ultimate goal is to assist elimination efforts by developing easy-to-use tools that incorporate genetic information about transmission and drug response for more effective mass drug distribution, surveillance strategies, and decisions on when to stop interventions to improve sustainability of elimination.
Herbarium Life: Shipping Cryptogamic Specimens
Creators: name: Tocci, Genevieve E.
Publisher: name: Zenodo
Year: 2022

description: Abstract of conference oral presentation on Herbarium Life: Shipping Cryptogamic Specimens by Genevieve E. Tocci of Harvard University Herbaria 22 Divinity Ave. United States for SPNHC2022 Conference, Edinburgh. June 5-10th 2022.
Mechanical Properties and Thermal Stability of methyl methacrylate grafted latex and natural rubber latex foam blends
Creators: name: Kanagaraj, Logan
name: AB Chai
name: SY Ch'ng
name: Kong, Ing
Publisher: name: La Trobe
Year: 2021
Subjects: Uncategorized

description: © School of Engineering, Taylor’s University. Graft copolymerisation has been and is still globally used as one of the main methods of modifying natural rubber in both latex form and solution. Plenty of researches can be found on the application of graft copolymerization to reinforce natural rubber latex, but research on incorporating grafted rubber into natural rubber latex foam (NRLF) is yet to be done. The purpose of this study was to investigate the effect of methyl methacrylate grafted rubber latex (MGL) loading on the mechanical properties of the compounded NRLF. The blended latex was prepared by mixing high ammonia natural rubber latex and the industry-standard polymethyl methacrylate (PMMA)-grafted natural rubber latex, referred to as MG49. Then, the blended latex was compounded and processed into NRLF at a laboratory scale via the Dunlop process. A comparative study between the NRLF produced with varying MGL percentage, i.e., 0% (control), 10%, 20%, 30%, 40% and 50% was done. The hardness, compression set and stiffness of the NRLF increased as the percentage of MGL content in the NRLF increased. The addition of MGL in the NRLF showed a decrease in the thermal stability of the NRLF for temperatures below 400 °C, but no significant trend for higher temperatures. NRLF samples with higher MGL content also showed a tendency to swell more when soaked in toluene.
Highly pathogenic avian influenza (A/H5N1) virus outbreaks in Lesotho, May 2021
Creators: name: Makalo, Mabusetsa R.J.
name: Dundon, William G.
name: Settypalli, Tirumala B.K.
name: Datta, Sneha
name: Lamien, Charles E.
name: Cattoli, Giovanni
name: Phalatsi, Moeketsi S.
name: Lepheana, Relebohile J.
name: Matlali, Mpaliseng
name: Mahloane, Relebohile G.
name: Molomo, Marosi
name: Mphaka, Palesa C.
Publisher: name: Taylor & Francis
Year: 2022
Subjects: subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Biotechnology
subject: Environmental Sciences not elsewhere classified
subject: Ecology
subject: Sociology
subject: FOS: Sociology
subject: Biological Sciences not elsewhere classified
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Virology

description: In May 2021, Lesotho reported its first outbreak of highly pathogenic avian influenza (HPAI) to the OIE. Samples were collected from infected poultry and the virus was confirmed by molecular tests to be of the H5N1 subtype. Full genome sequencing and phylogenetic analysis revealed that the viruses belonged to clade 2.3.4.4b and showed high identity with A/H5N1 viruses identified in Nigeria and Senegal in early 2021. The identification of A/H5N1 HPAI in Lesotho has important implications for disease management and food security in the region.
Study protocol for an evaluation of ASDetect - A Mobile application for the early detection of autism
Creators: name: Barbaro, Josephine
name: Yaari, Maya
Publisher: name: La Trobe
Year: 2022
Subjects: classificationCode: 3213
subject: Paediatrics
classificationCode: 42
subject: Health sciences
subject: FOS: Health sciences
classificationCode: 4203
subject: Health services and systems
classificationCode: 4204
subject: Midwifery

description: Background: Autism Spectrum Conditions (ASC) can be reliably diagnosed by 24 months of age. However, despite the well-known benefits of early intervention, there is still a research-practice gap in the timely identification of ASC, particularly in low-resourced settings. The Social Attention and Communication Surveillance (SACS) tool, which assesses behavioural markers of autism between 12 to 24 months of age, has been implemented in Maternal and Child Health (MCH) settings, with excellent psychometric properties. ASDetect is a free mobile application based on the SACS, which is designed to meet the need for an effective, evidence-based tool for parents, to learn about children's early social-communication development and assess their child's 'likelihood' for ASC. Study aims: The primary aim of this study is to evaluate the psychometric properties of ASDetect in the early detection of children with ASC. A secondary aim is to assess ASDetect's acceptability and parental user experience with the application. Methods: Families are recruited to download the application and participate in the study via social media, health professionals (e.g., MCH nurses, paediatricians) and word of mouth. All participating caregivers complete a demographic questionnaire, survey regarding their user experience, and the Social Responsiveness Scale-2 (SRS-2), an autism screening questionnaire; they are also invited to participate in focus groups. Children identified at 'high likelihood' for ASC based on the ASDetect results, the SRS-2 or parental and/or professional concerns undergo a formal, gold-standard, diagnostic assessment. Receiver Operating Characteristic analyses will be used to assess psychometric properties of ASDetect. Thematic analyses will be used to explore themes arising in the focus groups to provide insights regarding user experiences with the app. Multiple regression analyses will be carried out to determine the extent to which demographic factors, parental stress and beliefs on health surveillance and child results on ASDetect are associated with the parental user-experience of the application. Discussion: With a strong evidence-base and global access, ASDetect has the potential to empower parents by providing them with knowledge of their child's social-communication development, validating and reassuring any parental concerns, and supporting them in communicating with other health professionals, ultimately enhancing child and family outcomes and well-being.
Preclinical immunological evaluation of an intradermal heterologous vaccine against SARS-CoV-2 variants
Creators: name: Fan, Shengtao
name: Xiao, Kang
name: Li, Dandan
name: Zhao, Heng
name: Zhang, Jingjing
name: Yu, Li
name: Chang, Penglan
name: Zhu, Shuangli
name: Xu, Xingli
name: Liao, Yun
name: Ji, Tianjiao
name: Jiang, Guorun
name: Yan, Dongmei
name: Zeng, Fengyuan
name: Duan, Suqin
name: Xia, Baicheng
name: Wang, Lichun
name: Yang, Fengmei
name: He, Zhanlong
name: Song, Yang
name: Cui, Pingfang
name: Li, Xiaolei
name: Zhang, Yaxing
name: Zheng, Bangyi
name: Zhang, Ying
name: Xu, Wenbo
name: Li, Qihan
Publisher: name: Taylor & Francis
Year: 2022
Subjects: subject: Microbiology
subject: FOS: Biological sciences
subject: Genetics
subject: Biotechnology
subject: Immunology
subject: FOS: Clinical medicine
subject: Mathematical Sciences not elsewhere classified
subject: Cancer
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Virology
subject: Computational Biology

description: The recent emergence of COVID-19 variants has necessitated the development of new vaccines that stimulate the formation of high levels of neutralizing antibodies against S antigen variants. A new strategy involves the intradermal administration of heterologous vaccines composed of one or two doses of inactivated vaccine and a booster dose with the mutated S1 protein (K-S). Such vaccines improve the immune efficacy by increasing the neutralizing antibody titers and promoting specific T cell responses against five variants of the RBD protein. A viral challenge test with the B.1.617.2 (Delta) variant confirmed that both administration schedules (i.e. “1 + 1” and “2 + 1”) ensured protection against this strain. These results suggest that the aforementioned strategy is effective for protecting against new variants and enhances the anamnestic immune response in the immunized population.