cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Family-based investigation of the genetics and epigenetics of obesity in Qatar
Creators: name: Alshafai, Mashael Nedham A. J.
Publisher: name: Imperial College London
Year: 2015

description: Single nucleotide polymorphisms (SNPs), copy number variations (CNVs) and DNA methylation patterns play a role in the susceptibility to obesity. Despite the alarming figures of obesity in the Arab world, the genetics of obesity remain understudied in Arabs. Here, I recruited ten multigenerational Qatari families segregating obesity, and generated genome-wide SNP genotyping, whole genome sequencing and genome-wide methylation profiling data using Illumina platforms to investigate the role of rare mutations, CNVs, and DNA methylation patterns in the susceptibility to obesity. For the identification of obesity mutations, I first identified candidate obesity regions through linkage and run of homozygosity analyses, and then investigated these regions to detect potential deleterious mutations. These analyses highlighted putative rare variants for obesity risk at PCSK1, NMUR2, CLOCK and RETSAT. The functional impact of the PCSK1 mutation on obesity was previously demonstrated while for the other candidates further work is needed to confirm their impact. For the identification of common obesity CNVs, I performed a genome-wide CNV association analysis with BMI and identified a common duplication of ~5.6kb on 19p13.3 that associates with higher BMI. Moreover, I investigated large (≥500kb) rare CNVs and identified a ~618kb deletion on 16p11.2 in the most extremely obese subject in my samples, which confirms the contribution of the previously reported 16p11.2 deletions to severe obesity beyond European populations. For the identification of DNA methylation changes in obesity, I attempted to replicate known associations with BMI from large EWASs. I replicated the associations at ABCG1 (P-value=6.3X10-3) and CPT1A (P-value=8.7X10-5) and compared the effects observed to the TwinsUK cohort through a meta-analysis. I observed low heterogeneity between the two studies, and that increased the associations at ABCG1 (P-value=2.5x10-13) and CPT1A (P-value=1.9x10-15). In conclusion, the work represents the first genetic and epigenetic study of obesity in an Arab population. I replicated known genetic and epigenetic obesity susceptibility loci and also discovered novel potential loci.
CCWI2017: F40 'New test-rig for micro hydropower turbomachines'
Creators: name: Delgado, João
name: Ferreira, João P.
name: Dídia I.C. Covas
name: Avellan, François
Publisher: name: The University of Sheffield
Year: 2017
Subjects: subject: 90599 Civil Engineering not elsewhere classified
subject: FOS: Civil engineering

description: Energy recovery in water supply systems has been pointed as an interesting opportunity. The available powers for recovery are usually small and, therefore, there is the need to develop cost- effective solutions. Pumps running as turbines are pointed as a prospective solution for this purpose. However, there are still some challenges to address, namely the prediction of turbine mode performance. For this purpose, a new test-rig was assembled to test different pumps in turbine mode and operational constraints. This paper shows the details of the developed experimental facility, data acquisition and control system. Preliminary experimental results are presented for the efficiency hillchart, as well as the influence of backpressure in the pressure fluctuations downstream the pump. The development of this test-rig aims at collecting reliable data for developing formulations to accurately predict the pump as turbine performance.
CCWI2017: F17 'Assessment of alternatives for energy efficiency improvement using a hydraulic simulation model'
Creators: name: Santos-Tellez, Rodrigo Ulises
name: Rodríguez-Varela, José Manuel
name: Silvestre, Juan Maldonado
name: Tzatchkov, Velitchko G.
Publisher: name: The University of Sheffield
Year: 2017
Subjects: subject: 90599 Civil Engineering not elsewhere classified
subject: FOS: Civil engineering

description: Many water utilities that provide water, drainage and sanitation services in Mexico encounter several problems performing these duties. They face insufficient funds, frequent changes of administrative and qualified technical personnel, as well as distribution-network aging. This situation, along with high water losses in transmission pipelines and distribution networks, illegal service connections, and intermittent water supply have led to a large portion of the water utilities having to deal with a low willingness from the users to pay for the service provided. Pumping and water treatment make up most of the high electricity costs generated from the provision of water supply services. This paper presents the results of an energy efficiency study, using a hydraulic simulation model as a starting point in which various energy-consumption optimization scenarios were considered. Priority was placed on existing infrastructure use, the assessment of alternatives for pumping equipment operation, the addition of complimentary equipment with its corresponding capital and running costs as well as its repercussions on the service provided to end-users and finally their impact on the total running costs for the water utility. To carry out an adequate interpretation of the hydraulic model, it was necessary to perform a detailed analysis of existing pumping equipment, primarily its electromechanical parameters in such a way that upon integrating them into the model, the proposed alternatives carry the certainty of success. In general form, the analyzed sources of supply produce an average of 437 L/s, with an energy consumption of 920 kW/h. Based on the hydraulic simulation, it is possible to take 5 pumping sites out of operation from the Pump Stations G.O. 1 and 2 which implies a reduction of 121.7 kW/h and as such, considering the rate at the moment of this study which is $1.025 kW/h, yields an annual savings of $55,000.00 USD, representing a 24% decrease in the total energy consumed by the distribution network.
Additional file 2 of A sonosensitiser‐based polymeric nanoplatform for chemo‐sonodynamic combination therapy of lung cancer
Creators: name: Zhang, Yanan
name: Khan, Abdur Rauf
name: Yang, Xiaoye
name: Shi, Yikang
name: Zhao, Xiaogang
name: Zhai, Guangxi
Publisher: name: figshare
Year: 2021
Subjects: Space Science
Medicine
Cell Biology
Pharmacology
Biological Sciences not elsewhere classified
Cancer

description: Additional file 2. Described the experimental methods.
CCWI2017: F106 'Water and Energy Efficiency in Bulk Water Systems'
Creators: name: Monteiro, Laura
name: Mamade, Aisha
name: Figueiredo, David
name: Alves, Rita
name: Póvoa, Pedro
name: Dídia Covas
Publisher: name: The University of Sheffield
Year: 2017
Subjects: subject: 90599 Civil Engineering not elsewhere classified
subject: FOS: Civil engineering

description: Water losses and energy efficiency in supply systems is an actual concern for utilities. While the methodologies for water losses and energy efficiency assessment have been developed, their application in bulk water supply systems is limited. This paper provides data on water losses levels and energy efficiency performance of three Portuguese bulk water supply systems that can be benchmarked with similar systems worldwide. In addition, it points out major uncertainties in water balances calculation in such systems and identifies constraints in applying the methodology. The usefulness of computing the energy balance for efficiency assessment in bulk supply systems is demonstrated.
Modelling Crebbp loss in BCL2 driven non-Hodgkin’s lymphoma
Creators: name: Dawes, Joanna Camilla
Publisher: name: Imperial College London
Year: 2015

description: Non-Hodgkin’s lymphomas (NHL) are a spectrum of hematopoietic cancer accounting for 4% of new cancer diagnoses each year. Approximately 95% of all NHL are of B-cell origin; diffuse large B-cell lymphomas (DLBCL) and follicular lymphoma (FL) accounting for 30-40% and 20% of B-NHL respectively. Recent human mutation profiling and resequencing studies have shown that CREBBP and BCL2 are frequently mutated, early events in B-NHL that are often are concurrent. This thesis presents a study of their role in oncogenesis by generating a novel model of B-NHL overexpressing BCL2 in the haematopoetic compartment combined with the conditional heterozygous loss of Crebbp in the germinal centre B-cells. Loss of Crebbp significantly accelerated BCL2 driven lymphomagenesis. Characterisation of these lymphomas by flow cytometry demonstrated that they were phenotypically similar to mature human B-cell lymphomas of germinal centre origin. Additionally an insertional mutagenesis screen was performed in mice sensitised to B-NHL. Somatic MoMuLV caused mutations interact with the sensitising mutations, facilitating tumourigenesis and recapitulating the stepwise and accumulative progression of human disease. Provirus insertions were detected using a novel method of insertion site cloning called UMI-LM-PCR which amplifies the region flanking the provirus. Insertions were then mapped to the mouse genome flagging genes with a putative role in tumourigenesis. This method represents the highest throughput method to date and its applicability to Illumina sequencing permits the most comprehensive quantitative survey of subclonal mutations. This work confirms Crebbp is a tumour suppressor gene and that its loss cooperates with overexpression of BCL2 to accelerate lymphomagenesis. Through insertional mutagenesis screening of this model hundreds of putative cancer genes have been identified including Pou2f2 and Tfrc which have a propensity for mutation specifically in B-cell lymphomas deficient in Crebbp and overexpressing BCL2. These candidates now need to be validated to better characterise their role in B-cell lymphoma
Stelleninserate fair und attraktiv gestalten
Creators: name: Pässler, Katja
Publisher: name: Fachhochschule Nordwestschweiz FHNW
Year: 2021

description: Stelleninserate oder interne Stellenausschreibungen sind wichtige Instrumente, um geeignete Bewerber*innen zu rekrutieren. Ungünstige sprachliche Formulierungen und eine einseitige visuelle Gestaltung können Stereotype aktivieren und dazu führen, dass geeignete Kandidat*innen abgeschreckt werden. Wie Stelleninserate fair und attraktiv für unterschiedliche Zielgruppen gestaltet werden können veranschaulicht Dr. Katja Pässler in diesem Vortrag anhand der Bedeutung von genderfairen Stelleninseraten für die Rekrutierung weiblicher Fach- und Führungskräfte.
Who is able to help? Inherent dispositions (svabhāva) in healing – Ācārya dr. Sthaneshwar Timalsina
Creators: name: Timalsina, Staneshwar
Publisher: name: Zenodo
Year: 2022
Subjects: Kali
mahadeva
agama
Mahakali
advaita vedanta
Kubjika
Shiva Sutra
Kashmir Shaivism
Kula
sarvamnaya
Saundaryalahari
krama
Camunda
Shiva
vimarsha-foundation
tantraloka
Spanda
Sadāśiva
Tripura Rahasya
vedanta
Abhinavagupta
saivadvaita
shakti
Guhyakālī
sthaneshwar timalsina
Durga
Bhairava
trika
Divine Mother
shambhava
Tantra
Devi Mahatmya
Tripurasundari
Sarvamnaya
timalsina
tripura
Mālinīvijayottaratantra
consciousness
Kaula
anava upaya
yantra
Īśāna
Vasugupta
shakta upāya
Chandi
Shaiva
sarvamnaya-oral-tradition-archive
Tantric philosophy
amnaya

description: Following a talk on "advaita darśan and Bhagavad gītā" a novice doctor is asking Ācārya ji, how she can guide the patients to be able to recognise and then to take the right direction in their lifes.

NEXT FREE COURSE offered by Śaivācārya Prof. Sthaneshwar Timalsina: https://www.vimarshafoundation.org/courses

Original video: https://www.youtube.com/watch?v=HgfOb11WXws&list=PL9t2HU2EqAuzyMfVvEB0JJa0wJfKIJrt8&index=37&t=56s

Original YouTube video: https://www.youtube.com/watch?v=vYKDLilHWV4

This presentation is part of the Sarvāmnāya Oral Tradition Archive.
Vimarsha Foundation. (2025). The Sarvāmnāya Oral Tradition Archive [Data set]. Zenodo. https://doi.org/10.5281/zenodo.15188107
Improving outcomes in knee arthroplasty: the lateral unicompartmental knee replacement
Creators: name: Newman, Simon David Standen
Publisher: name: Imperial College London
Year: 2016

description: Total knee replacement (TKR) continues to increase in popularity, but satisfying patients who remain active for longer is a major challenge. Outcomes from TKR remain suboptimal, with as many as 20% gaining little benefit. An alternative approach to TKR is the use of partial or unicompartmental knee replacements (UKR). These are smaller and safer operations, but are arguably more difficult to perform and have been associated with a higher rate of revision surgery. Whilst a great deal is understood about medial UKR, relatively little is known about lateral UKR and this procedure forms the basis of this thesis. Outcomes of a lateral UKR implant, the Oxford Domed Lateral Partial Knee were assessed through upto 10 year follow-up of the largest independent series of this implant and demonstrated similar rates of revision and Oxford Knee Scores to previously published medial UKR series. Further assessment of the gait of patients with the same implant however failed to show a significant improvement over demographically matched patients with TKR. Accuracy of implant positioning is important for achieving optimal outcomes. One technology that may assist in delivering expert level skill is patient specific instrumentation (PSI). For the first time, the effect of changes in PSI guide design were explored, demonstrating the importance of achieving multiplanar bone contact. The use of PSI guides compared to traditional instruments was associated with superior femoral implant positioning and a trend for superior tibial implant positioning when used by inexperienced surgeons on a training course. The effect of tibial implant malpositioning on tibial strain was demonstrated using digital image correlation. Excessive posterior slope and tibial resection should be avoided to prevent higher tibial strains that may result in fracture. A pre-requisite for successful arthroplasty, including lateral UKR, is the establishment of osseomechanical integration. Enhanced osseomechanical integration may be better achieved with strontium-substituted bioactive glass (SrBG) than a hydroxyapatite (HA) coating as demonstrated in a lapine model. The data presented in this thesis demonstrates that lateral UKR is a challenging but effective operation, though further development is required to facilitate normal gait. Further improvements in function and outcomes may be achievable through the use of PSI and enhanced implant fixation with SrBG coatings.
"XIRADNOMAYI ISKANDARIY" VA "SADDI ISKANDARIY" DOSTONLARINING SHAXS MA'ANAVIY KAMOLOTIGA TA'SIRI
Creators: name: Otayarova, Umida
Publisher: name: Zenodo
Year: 2024

description: Ushbu maqolada Abdurahmon Jomiyning “Xiradnomayi Iskandariy” va Alisher Navoiyning “Saddi Iskandariy” dostonlaridagi insonparlik g‘oyalari hikoyatlar misolida ochib berilgan.
Understanding and optimising HIV prevention and related outcomes among serodiscordant couples in Africa
Creators: name: Jewell, Britta
Publisher: name: Imperial College London
Year: 2015

description: Daily oral tenofovir-based pre-exposure prophylaxis (PrEP) has recently been shown to be an efficacious HIV prevention strategy for key populations at high risk of acquiring HIV-1, including serodiscordant couples. This thesis addresses the following research question: what is the projected impact and cost-effectiveness of PrEP for serodiscordant couples in sub-Saharan Africa, and would it be a worthwhile intervention? By using statistical analysis and mathematical modelling, I answer this question and provide recommendations for the optimisation of antiretroviral-based HIV prevention this population. In this thesis, I begin by exploring the effect of the reduced risk of herpes simplex virus type 2 (HSV-2) acquisition by means of PrEP on the outcomes of an intervention, but conclude that the supplementary benefit is unlikely to substantially alter the costeffectiveness. I also perform statistical analysis of daily adherence patterns to PrEP, according to electronic monitoring, from the Partners PrEP Study and the Partners Demonstration Project in Kenya and Uganda and find that adherence was high in both settings. Finally, I adapt a microsimulation model of serodiscordant couples in Africa to include realistic PrEP adherence patterns, HIV-1 transmission, external partnerships, and empirical costs. Using this model, I simulate the likely impact and cost-effectiveness of a PrEP intervention according to characteristics and behaviours of couples in the context of a clinical trial and a demonstration project. Overall, I conclude that a PrEP intervention could be cost-effective or cost-saving when targeted to high-risk couples, but is unlikely to be cost-effective if expected incidence is low. In addition, the utility of PrEP for serodiscordant couples could be compromised if the resources needed to find high-risk couples are expensive or if couples are not found easily.
Photoredox-Catalyzed Site-Selective Generation of Carbanions from C(sp3)–H Bonds in Amines
Creators: name: Murugesan, Kathiravan
name: Donabauer, Karsten
name: Narobe, Rok
name: Derdau, Volker
name: Bauer, Armin
name: König, Burkhard
Publisher: name: Universität Regensburg
Year: 2022
Subjects: subject: C-H; C(SP(3))-H BONDS; ATOM TRANSFER; FUNCTIONALIZATION; DEUTERATION; ALDEHYDES; EQUIVALENTS; ALLYLATION; TRITIATION; ALCOHOLS; photoredox catalysis; carbanion; site-selectivity; isotope labeling; amino alcohols; C-H functionalization
subject: 540 Chemie

description: The selective activation of sp3 carbon-hydrogen bonds in the presence of multiple C-H bonds is challenging and remains of supreme importance in chemical research. Late-stage modification of complex molecules via sp3 C-H activation is of high prevalence in organic synthesis. Herein, we describe the activation of a C(sp3 )-H bond in the alpha-position to an amine via a carbanion intermediate. In the presence of several alpha-amine sites, only one specific position is selectively activated. Applying this protocol, the proposed carbanion intermediate was effectively trapped with different electrophiles such as deuterium (D+ ), tritium (T+ ), or carbonyl compounds compiling over 50 examples. Further, this methodology was used to install deuterium or tritium in different drug-derivatives (>10 drugs) at a selected position in a late-stage functionalization. In addition, the protocol is suitable for a gram-scale synthesis, and a detailed mechanistic investigation has been carried out to support our hypothesis.
CCWI2017: F87 'Infection Delay Time Analysis for Enhancing Water Networks Vulnerabilities to Contamination Intrusions'
Creators: name: Lifshitz Ron
name: Ostfeld Avi
Publisher: name: The University of Sheffield
Year: 2017
Subjects: subject: 90599 Civil Engineering not elsewhere classified
subject: FOS: Civil engineering

description: In water distribution systems, the prediction of infection spread along the system's components is critical both for minimizing impact on the system population and for real-time response. Delaying the overall spread of an infection from a single or from multiple infection sources can be a very useful tool for handling such contamination scenarios. In this study, a new parameter for Infection Delay Time (IDT) is presented and the methodology utilizing it is presented on simple and complex networks, along with highlighting the IDT advantages and applications for system’s design and operation.
CCWI2017: F13 'Reduction of Infection Risk by Automated Rapid Detection of Faecal Contamination of Drinking Water Distribution Systems'
Creators: name: E.J.M. Blokker
name: P.W.M.H. Smeets
Publisher: name: The University of Sheffield
Year: 2017
Subjects: subject: 90599 Civil Engineering not elsewhere classified
subject: FOS: Civil engineering

description: Water companies may perform a quantitative microbial risk assessment to verify that the produced water is safe to drink. However, in the drinking water distribution system (DWDS) a faecal contamination may occur when the integrity of the system is broken. Therefore a similar verification of water safety during distribution is desirable, however challenging due to the complexity and extent of the network. Currently verification consists of regulatory 100 ml samples for E.coli and enterococci analyses at the consumer tap. The drawbacks are that not all contaminations are detected and analysis results take 24 to 48 hours, before action can be taken. Automated analysers that take a sample and analyse it on location within a few hours are now available and can act as online sensors for faecal contamination. This allows a much faster response when a contamination is detected. In this study a hydraulic model was run with many contamination scenario’s and sensor networks to quantify the resulting exposure of consumes to pathogens. The resulting infection risk was then determined with a previously developed QMRA model for distribution systems. It was shown that a boil water notice within hours instead of days can significantly reduce the infection risk. Next to reducing infection risk, these automated analysers can help to better assess infection risk levels and enable more flexibility in network maintenance.
CCWI2017: F98 'Two-Point Constraint Control of Water Quality in Distribution Networks'
Creators: name: Maheshwari, Abhilasha
name: Abokifa, Ahmed A.
name: Ravindra D. Gudi
name: Pratim Biswas
Publisher: name: The University of Sheffield
Year: 2017
Subjects: subject: 90599 Civil Engineering not elsewhere classified
subject: FOS: Civil engineering

description: Providing potable drinking water, with sufficient free residual chlorine to prevent microbial regrowth and contaminant by-product formation, is of utmost importance for municipal authorities. This work aims towards water quality control as it transports through water distribution networks. Presence of excessive chlorine (used as disinfectant worldwide) in water causes the generation of carcinogenic disinfectant by-products such as Tri-halo methane (THMs). On the other hand, lower chlorine levels in water results in microbial contamination. Hence, this leads to a two-point constraint control problem of maintaining free residual chlorine levels under the upper and lower bounds, by suitably optimizing the disinfectant dosage at booster stations. However, transport delay and complex interrelations present amongst the nodes in large water distribution network, makes it difficult to design a global feedback control system. Therefore, in this work we have proposed to decentralize the system by clustering demand nodes of the network based on concentration sensitivity matrix analysis. Further, input-output pairs for decentralized controllers were mapped with the knowledge of interactions between manipulating and control variables using partial correlation analysis. We also considered the results of partial correlation analysis to generate communication links which will be implemented for higher level co-ordinated decentralized control to account for dynamic, non-negligible interactions as disturbances to local control units. The proposed three step clustering-mapping strategy and mathematical formulation for two point control by optimized dosage is successfully verified for the steady state case on a prototype distribution example network.
Smart scheduling recommender system for process-centric production planning in medium sized enterprises (SRS-4-MSE)
Creators: name: Waldburger, Raoul
Publisher: name: Fachhochschule Nordwestschweiz FHNW
Year: 2021
Subjects: subject: Production planning
subject: Process mining
subject: 600 - Technik, Medizin, angewandte Wissenschaften

description: SRS-4-MSE is focusing on a process-centric scheduling for MSE in the MEM sector by combining process mining and process simulation algorithms in a recommender system to reduce throughput times with the application of a digital twin. In the Swiss mechanical and electrical engineering industries (MEM), usually ERP modules for scheduling processes based on heuristics are applied in the production planning. Reduction of throughput time with smart digital twins in the production allows for an increase regarding flexibility and responsiveness as well as higher delivery readiness (reduced out-of-stock). First analysis results of the PoC performed with an anonymized offline data set indicated a reduction potential of average throughput times of over 50%.
title: Data Stewardship Maturity Report for JPSS Microwave Integrated Retrieval System (MiRS) Advanced Technology Microwave Sounder (ATMS) Precipitation and Surface Products
Creators: name: Hager, Lori
Publisher: name: National Centers for Environmental Information (U.S.)
Year: 2021

description: NOAA Technical Information Series NESDIS DSMR-00254
The contribution of the cell cycle to reprogramming and the role of Jarid2 in coordinating Nanog expression and Wnt/PCP signalling in mESC differentiation and early development
Creators: name: Malinowski, Andrzej
Publisher: name: Imperial College London
Year: 2015

description: It remains unclear how cellular identities are established during developmental programming and reprogramming and what the contributing factors are. Pluripotent cells were shown to dominantly reprogram somatic nuclei towards pluripotency upon experimental cell fusion, but the role of the cell cycle in determining dominance has not been explored. I compared the reprogramming capacity of two pluripotent cell types, epiblast stem cells (EpiSCs) and embryonic stem cells (ESCs) reported to show different cell cycle structures. I demonstrated that compared to ESCs EpiSCs induce lower levels of pluripotency-associated transcripts in somatic nuclei, but activate the expression of genes associated with early differentiation programs. This reduced pluripotent reprogramming capacity of EpiSCs is not due to differences in the cell cycle as these cell types show similar cell cycle structures. Increasing Nanog expression is not sufficient to enhance the pluripotent reprogramming capacity of EpiSCs. Subsequently, I examined whether fusing S-G/2 somatic cells with G1 mESCs could reverse the dominance and reprogram mESCs towards somatic-like identity. I show that B lymphocytes do not induce reprogramming of ESCs nuclei towards lymphocyte-like state, regardless of cell cycle stages of these cell types. Establishment of new cellular identities can be also examined by ESC differentiation. Jarid2 is a member of Polycomb Repressor Complex 2 (PRC2) maintaining gene repression via chromatin regulation. Jarid2 deficiency does not cause major chromatin alterations, but results in impaired differentiation. In the second part of my thesis, I investigated factors and pathways deregulated in Jarid2-null mESCs. I show that Jarid2-null ESCs express constitutively high levels of Nanog, but reduced β-catenin activity and expression of PCP signalling components. Co-culture of Jarid2-null with wild-type mESCs reinstates normal Nanog expression and β-catenin activity, and partially restores the differentiation capacity of mutant cells. These results reveal a role for Jarid2 in coordinating a core pluripotency and Wnt/PCP signalling network, and facilitating ESC differentiation.
Linezolid brain penetration in neurointensive care patients
Creators: name: Hosmann, Arthur
name: Moser, Miriam M
name: van Os, Wisse
name: Gramms, Leon
name: al Jalali, Valentin
name: Sanz Codina, Maria
name: Plöchl, Walter
name: Lier, Constantin
name: Kees, Frieder
name: Dorn, Christoph
name: Rössler, Karl
name: Reinprecht, Andrea
name: Zeitlinger, Markus
Publisher: name: Universität Regensburg
Year: 2024
Subjects: subject: 615 Pharmazie

description: Background: Linezolid exposure in critically ill patients is associated with high inter-individual variability, potentially resulting in subtherapeutic antibiotic exposure. Linezolid exhibits good penetration into the CSF, but its penetration into cerebral interstitial fluid (ISF) is unknown.

Objectives: To determine linezolid penetration into CSF and cerebral ISF of neurointensive care patients.

Patients and methods: Five neurocritical care patients received 600 mg of linezolid IV twice daily for treatment of extracerebral infections. At steady state, blood and CSF samples were collected from arterial and ventricular catheters, and microdialysate was obtained from a cerebral intraparenchymal probe.

Results: The median fAUC0-24 was 57.6 (24.9-365) mg·h/L in plasma, 64.1 (43.5-306.1) mg·h/L in CSF, and 27.0 (10.7-217.6) mg·h/L in cerebral ISF. The median penetration ratio (fAUCbrain_or_CSF/fAUCplasma) was 0.5 (0.25-0.81) for cerebral ISF and 0.92 (0.79-1) for CSF. Cerebral ISF concentrations correlated well with plasma (R = 0.93, P < 0.001) and CSF levels (R = 0.93, P < 0.001).The median fAUC0-24/MIC ratio was ≥100 in plasma and CSF for MICs of ≤0.5 mg/L, and in cerebral ISF for MICs of ≤0.25 mg/L. The median fT>MIC was ≥80% of the dosing interval in CSF for MICs of ≤0.5 mg/L, and in plasma and cerebral ISF for MICs of ≤0.25 mg/L.

Conclusions: Linezolid demonstrates a high degree of cerebral penetration, and brain concentrations correlate well with plasma and CSF levels. However, substantial variability in plasma levels, and thus cerebral concentrations, may result in subtherapeutic tissue concentrations in critically ill patients with standard dosing, necessitating therapeutic drug monitoring.
Development of a bile acid biosensor for use in the clinical diagnosis of cholestasis
Creators: name: Lawrance, Dominic
Publisher: name: Imperial College London
Year: 2012

description: This thesis describes the development of a disposable, point-of-care biosensor for measurement of total serum bile acids. The condition known as intrahepatic cholestasis of pregnancy was the motivation behind the development of the sensor. Raised serum bile acids in the absence of any liver disorder are an indicator of cholestasis. During pregnancy high bile acid levels are associated with an increased risk of adverse fetal outcomes. Current methods for analysing bile acid levels require samples to be processed by trained personnel with a time to result from hours to days. The aims of this project therefore were to assess the feasibility of an in situ measurement system by which a patient could be diagnosed rapidly and without the need for a laboratory setting. The first chapter presents background information and introduces key areas of relevance to the rest of the thesis. The second chapter outlines all of the materials and methods used in order to obtain the results described herein. The third chapter concentrates on the electrode reaction which forms the basis for detection. The fourth chapter focuses on the development of the biosensor and the key enzymatic reaction which confers specificity to bile acids. The fifth chapter presents clinical results obtained using the biosensor compared with the standard clinical values. The sixth chapter summarises the project and discusses future development and how this research can be utilised. The original contributions to scientific knowledge are: • A disposable biosensor that, unlike other sensors in the literature, is reported working with untreated serum samples to detect bile acids at clinically relevant levels • A feasibility study demonstrating the use of the biosensor with clinical samples emphasising the expedience of the biosensor as an in situ tool for measurement of serum bile acids • A previously unreported method for carrying out electrochemical assays involving the standard addition of bile acids to calibrate the biosensor and account for variations in sensitivity.
Einfluss der Wärmespeicherfähigkeit auf die energetische Flexibilität von Gebäuden
Creators: name: Hall, Monika
name: Geissler, Achim
Publisher: name: Economic Forum
Year: 2015
Subjects: subject: Wärmespeicherfähigkeit
subject: Gleichzeitigkeit von Energiewandlung und Bedarf
subject: Netzinteraktion
subject: Thermische Gebäudesimulation
subject: 690 - Hausbau, Bauhandwerk

description: Üblicherweise speisen Gebäude mit Photovoltaikanlagen einen Grossteil des Ertrags in das öffentliche Stromnetz ein. Um den Eigenverbrauch zu erhöhen, bietet es sich bei gut gedämmten Gebäuden an, die Laufzeit der Wärmepumpe auf die Tageszeit zu limitieren. In diesem Zusammenhang wird der Einfluss der Wärmespeicherfähigkeit des Gebäudes auf die operativen Temperaturen in Abhängigkeit von verschiedenen Laufzeiten der Wärmepumpe mit einer thermischen Gebäudesimulation untersucht und mit Messwerten von einem kleinen Mehrfamilienhaus validiert. Es wird gezeigt, dass auch bei einem sehr gut gedämmten Gebäude eine mittlere bis hohe Wärmespeicherfähigkeit vorhanden sein muss, damit die Laufzeit der Wärmepumpe ohne Komforteinbusse auf die Tagesstunden limitiert werden kann. Mit einem Leichtbau ist die gewählte Strategie nicht möglich. Bei schweren Gebäuden kann die Dauer und der Zeitpunkt des Energieeinsatzes für die Wärmebereitstellung ohne Komforteinbusse an verschiedene Bedingungen angepasst werden, wie z.B. an Zeiten mit Solarangebot oder Zeiten mit günstigen Stromtarifen. Schwere Gebäude weisen eine höhere Flexibilität als Leichtbauten auf. Eine lange tägliche Sperrzeit führt gegenüber einem kontinuierlichen Betrieb zu grösseren Schwankungen der operativen Temperatur. Das Niveau und die Breite des zulässigen Temperaturbandes müssen mit den normativen Anforderungen für die Behaglichkeit abgestimmt sein. Die Diskussion über normative Behaglichkeitsanforderungen versus energetische Flexibilität des Gebäudes muss lanciert werden.
Definition of the myometrial transcriptome and proteome of preterm labour of known and unknown aetiologies versus term labour: elucidation of common vs. distinct regulatory pathways
Creators: name: Singh, Natasha
Publisher: name: Imperial College London
Year: 2015

description: Term labour (TL) is a complex interplay of maternal and fetal factors which maintains the uterus in a state of quiescence during pregnancy, and then subsequently converts the uterus into a contractile organ which aids to expel the baby. Preterm labour (PTL) is not a single entity but is now recognised as multifactorial disorder and not just the early onset of TL. The aim of this study was to examine the similarities and differences in the molecular pathways of both TL and PTL. Myometrial samples were collected at the time of caesarean section from women in no labour (preterm singleton, preterm twins and term), term early labour, term established labour and PTL (placental abruption, chorioamnionitis PTL, idiopathic PTL and Twins PTL) were used to study pro-labour gene expression using quantitative rtPCR and protein levels/concentrations using Western blotting and multiplexed cytokine assays. Increasing gestation was associated with increased OTR, CX-43, PGDS, PGES-1 and PGES-2 but a decrease in PGFS, PGIS, SRC3 and FKBP5. At the onset of TL (term early labour samples), mRNA expression of PGHS-2 and SRC3 increased while both mRNA expression and protein levels of PGDS, PGIS and PR-B decreased compared to term no labour (TNL). None of the inflammatory chemokines were increased at the onset of term labour compared to TNL. Established labour was associated with a decrease in PGHS-2 (mRNA), increased PGFS and a higher protein concentration of IL-1β, IL-4, IL-6, IL-8, IL-10, CCL-1, CCL-11, CCL-24, CXCL-1, CXCL-5 and CXCL-6 compared to TNL and term early labour. In the preterm labour phenotypes, chorioamnionitis PTL was associated with increased PGHS-2 (mRNA), OTR, PGES-2 and 14 cytokines but a decreased PGDS and CS-43 compared to preterm no labour (PTNL). In contrast, idiopathic PTL, polyhydramnios and abruption PTL was not associated with an increase in cytokines and showed different prolabour gene profiles compared to each other. PTL associated with twins had a less intense inflammatory profile than chorioamnionits PTL. The prolabour gene and inflammatory profile of TL differs in comparison to each of the PTL phenotypes. The inflammation in the myometrium in TL is a consequence rather than the cause of labour while that in PTL is most likely involved in the onset of PTL in phenotypes such as chorioamnionitis or twins PTL.
A metabonomic approach to the evaluation of xenobiotic metabolism and toxicity
Creators: name: Adesina-Georgiadis, Kyrillos N.
Publisher: name: Imperial College London
Year: 2015

description: This thesis comprises of studies which collectively describe the development and application of novel metabonomic approaches to the investigation of xenobiotic metabolism and toxicity. Both computational and UPLC-MS technologies are critically evaluated to facilitate the development of rapid, robust and high throughput analytical approaches for the analysis of compounds in biological matrices, particularly applicable in the area of drug discovery. Pattern recognition statistical modelling approaches were employed on both a small scale metabolism study of diclofenac and a large toxicology of acetaminophen. This methodology highlighted large differences between the test groups in both studies; however these discriminations were only compound related in the latter study. In a study with low statistical power, the probability of biologically unrelated processes, overwhelming the statistical significance of the compound related effects are quite high. Application of statistical normalisation for the correction of batch effects was also performed in an attempt to emolliate the issue of limited continuous LC-MS instrumental capacity. Batch correction was attempted using variance stabilisation with a glog function prior to median fold change normalisation. However, this approach was unable to correct the data to the extent where it was suitable for combined downstream analysis. A rapid gradient microbore UPLC-MS method is then described to provide a high-throughput solution for metabolic phenotyping of large sample collections. This method increases the flow rate, decreases the column diameter and the gradient elution time, reducing the length of the analysis of each individual while retaining the diagnostic capability of the experiment. The combined insights gained through this method development process are then applied to two separate toxicity studies of 2-bromophenol, one of acute and one of chronic exposure. UPLC-MS analysis coupled with 1H NMR spectroscopy identified the major metabolites as the sulphate and glucuronide conjugates, and other changes in endogenous metabolites. However the lack of reactive intermediates or other toxic metabolites indicate that 2-bromophenol is not a nephrotoxin. While these studies failed to discover any compound related toxicity, they have exemplified the utility of the metabonomic approach in this area.
Vorschriften evaluieren und sinnvoll reduzieren - aber wie? Entwicklung eines theoriegeleiteten und kriterienbasierten Prozesses zur Evaluation und Reduktion von Vorschriften
Creators: name: Hostettler, Karin
name: Nisoli, Giulio
name: Brüngger, Jonas
name: Wäfler, Toni
name: Fischer, Katrin
Publisher: name: GfA Press
Year: 2019
Subjects: Vorschriften
Vorschriftenreduktion
Standardisierung
Flexibilisierung
Resilienz

description: In Unternehmen mit hohem Gefahrenpotenzial sind Flexibilität und Resilienz wichtige Voraussetzungen, um Sicherheit zu gewährleisten. Aber gerade diese Unternehmen sehen sich einer zunehmenden Flut von Standardisierungen und Regulierungen durch Vorschriften gegenüber. Die Regeldichte hat inzwischen so stark zugenommen, dass Vorschriften oft nicht mehr vollständig eingehalten werden können (Dekker 2003; Hale & Borys 2012). Die kritische Reflexion (zu) vieler Vorschriften braucht theoretisch begründete Kriterien, mit denen Vorschriften auf ihre Eignung geprüft und ggf. reduziert werden können. Ziel der Reduktion ist ein System, das durch eine angemessene Balance von Standardisierung und Flexibilisierung anpassungsfähig wird und dabei die Expertise der Mitarbeitenden berücksichtigt. In einem Forschungsprojekt gemeinsam mit zwei Schweizer Bahnunternehmen und einer Aufsichtsbehörde wurden Kriterien zur Evaluation und Reduktion von Vorschriften aus arbeitspsychologischen Theorien abgeleitet. Ein auf diesen Kriterien basierender generischer und partizipativer Evaluationsprozesses wurde mit Vorschriftenerstellern, -anwendern und -überwachern entwickelt und getestet. Reduktionsstrategien für Vorschriften sind dabei die Elimination, die Substitution und die Flexibilisierung. Wann welche Strategie angewendet werden darf, wird im Beitrag beschrieben. So sollen Fachexperten bei der theoretisch begründeten Reduktion von Vorschriften und dem Erreichen einer angemessenen Balance von Standardisierung und Flexibilisierung im Unternehmen unterstützt werden.
Elucidating Schwann cell reprogramming
Creators: name: Byrne, Elizabeth
Publisher: name: Imperial College London
Year: 2016

description: The peripheral nervous system, unlike the central nervous system, has an exceptional capacity for regeneration following injury. This is due to the remarkable plasticity of the Schwann cells (SC), which are able to reprogramme, following injury, to a progenitor like cell which facilitates peripheral nerve repair. Current knowledge on the molecular basis of this reprogramming is incomplete and we are lacking a global overview of the transcriptional events that occur in SC following nerve injury and how these change over time. We aimed to characterise transcriptional changes in the SC, over time, following nerve injury using RNAseq. We also aimed to develop an in vitro dedifferentiation assay to use as a screening tool to asses potential key genes found using RNAseq. We developed a method of reliably extracting good quality, SC specific, RNA from the sciatic nerve of mice using fluorescence activated cell sorting. We performed RNAseq on SC from intact nerves and from the distal stump of nerves 6 days post transection. We validated this method by confirming differential expression of genes known to be up and downregulated following nerve injury, using RNAseq data. In analysing the RNAseq data we identified several potentially exciting, novel key molecular players in SC reprogramming, namely Myc and Runt-related transcription factor 1. We also developed an in vitro dedifferentiation assay to use as an initial screen for the genes identified using RNAseq. This involved the addition of neuregulin and serum to previously cyclic adenosine monophosphate differentiated SC. This assay was shown to recapitulate the changes seen in vivo, using RNAseq.
Berufliche Teilhabe messen und steuern (BWMK)
Creators: name: (:Unkn) Unknown
Publisher: name: Fachhochschule Nordwestschweiz FHNW
Year: 2019
Subjects: subject: 300 - Sozialwissenschaften

description: Bei der Steuerung von Werkstätten für Menschen mit Behinderungen und Beeinträchtigungen stehen sich drei Prozesse mit Zielkonflikten gegenüber. Der zentrale Prozess 'Teilhabe' muss gemäss der UN-Behindertenrechtskonvention eine möglichst normalisierte berufliche Teilhabe sicherstellen. Beim Prozess 'Arbeit' stehen betriebswirtschaftliche Faktoren im Zentrum. Weiter ist bei Werkstätten der Prozess 'Hilfe zur Teilhabe' von Bedeutung. In vielen Einrichtungen der Behindertenhilfe ist eine Entkoppelung der Prozesse und eine starke Fixierung der Steuerungsbestrebungen auf den Prozess 'Arbeit' festzustellen. Die Untersuchung in Einrichtungen in der Schweiz und Deutschland zeigt, dass die drei Prozesse durchaus steuerbare Verbindungen aufweisen, dies aber in den Einrichtungen anders wahrgenommen wird. Die Einrichtungen stabilisieren sich in Anbetracht der hoch gesteckten normativen Vorgaben und verbleiben in der scheinbar bewährten Praxis. Dabei hätten sie bereits jetzt Vieles zu bieten, beispielweise was normalisierte Arbeitsbedingungen betrifft. Auf die wichtige Frage, wie sich die Einrichtungen nun aus der Stabilisierung lösen können und anstehende Entwicklungsprozesse an definierten Wirkungszielen ausrichten, die sie sich selber geben oder von den normativen Vorgaben beziehen, entwickelt die Studie eine ganze Reihe an konkreten Antworten. Auch auf das Anliegen vieler Einrichtungen, wie die hergestellte Qualität der Teilhabe in einer Werkstätte für Menschen mit Behinderungen und Beeinträchtigungen gemessen und dargestellt werden kann.
title: Data Stewardship Maturity Report for GHRSST Level 4 AVHRR_AMSR_OI Global Blended Sea Surface Temperature Analysis (GDS version 1)
Creators: name: Ionin, Raisa
Publisher: name: National Centers for Environmental Information (U.S.)
Year: 2021

description: NOAA Technical Information Series NESDIS DSMR-00264
Kann man Lernen sichtbar machen?
Creators: name: Beywl, Wolfgang
Publisher: name: Schulverlag plus
Year: 2014
Subjects: subject: Ergebnisse der Hattie Studie
subject: Lernen sichtbar machen
subject: Lehren und Lernen sichtbar machen
subject: Interview
subject: Lehrpersonenhandeln
subject: Lernerfolg optimieren
subject: Feedback
subject: Selbstevaluation
subject: 370 - Erziehung, Schul- und Bildungswesen

description: «profil» führte mit Professor Wolfgang Beywl ein Gespräch zu den Ergebnissen der Hattie-Studie. Wolfgang Beywl ist einer der Übersetzer und Bearbeiter der beiden Bücher «Lernen sichtbar machen» und «Lernen sichtbar machen für Lehrpersonen».
title: Διερεύνηση εφαρμογής των οδηγιών της FIA για τον σχεδιασμό αγωνιστικής πίστας Κατηγορίας 1 στην Ελλάδα
title: Investigating the implementation of the FIA guidelines for the design of a Grade 1 race track in Greece
titleType: AlternativeTitle
Creators: name: Σιαφάκας, Ηλίας
Publisher: name: Πανεπιστήμιο Δυτικής Αττικής
Year: 2022
Subjects: Οδοποιία
Αγωνιστική πίστα
Φωτορεαλιστικά σχέδια
Οδόστρωμα
Αποτύπωση
FIA
Φόρμουλα 1
Οδοστρωσία

description: Στο παρόν τεύχος παρουσιάζονται οι προδιαγραφές της FIA που πρέπει να τηρούνται για την κατασκευή αγωνιστικής πίστας Κατηγορίας 1, πίστα δηλαδή που θα μπορεί να φιλοξενεί και αγώνες Φόρμουλα 1, όπως η γεωμετρική διάταξη, καθώς και τα μέτρα ασφαλείας που χρειάζονται για τη διεξαγωγή των μηχανοκίνητων αγώνων. Στη συνέχεια , πραγματοποιείται μελέτη και ανάλυση των γεωμετρικών χαρακτηριστικών τεσσάρων υφιστάμενων αγωνιστικών πιστών . Αυτές οι πίστες είναι αυτές του Σίλβερστον, του Ιντερλάγκος, του Μπαχρέιν και της Ίμολα. Για την ανάλυση τους αποτυπώνονται μιας και δ εν είναι γνωστά τα γεωμετρικά τους στοιχεία με τη χρήση του Plex Earth, μέσω του οποίου αποτυπώνονται οι πίστες στοAutoCAD σε κλίμακα 1:1. Στη συνέχεια, συσχετίζεται μαθηματικά η ακτίνα στροφής μιας πίστας με την ταχύτητα των αγωνιστικών αυτοκινήτων. Από αυτή τη συσχέτιση προκύπτει μια μαθηματική εξίσωση με την οποία μπορεί να γίνει μία βάσιμη εκτίμηση της ταχύτητας των αγωνιστικών αυτοκινήτων στις στροφές. Οι ταχύτητες των αγωνιστικών αυτοκινήτων που σχολιάζονται είναι αυτοκίνητα κατηγορίας Φόρμουλα 1. Έπειτα, προτείνεται η κατασκευή αγωνιστικής πίστας Κατηγορίας 1 στην Ελλάδα που θα πληρεί πλήρως τις προδιαγραφές και θα δύναται να είναι εντελώς λειτουργική. Παρουσιάζονται αναλυτικά τα σχέδια της, όπως ορίζει η FIA. Γίνεται και μία μελέτη όσο αναφορά για την εύρεση της ιδανικής τοποθεσίας καθώς και για τη σύνδεσή της με το ήδη υπάρχον οδικό δίκτυο.
Additional file 6 of Predicting neurological outcome after out-of-hospital cardiac arrest with cumulative information; development and internal validation of an artificial neural network algorithm
Creators: name: Andersson, Peder
name: Johnsson, Jesper
name: Björnsson, Ola
name: Cronberg, Tobias
name: Hassager, Christian
name: Zetterberg, Henrik
name: Stammet, Pascal
name: Undén, Johan
name: Kjaergaard, Jesper
name: Friberg, Hans
name: Blennow, Kaj
name: Lilja, Gisela
name: Wise, Matt P.
name: Dankiewicz, Josef
name: Nielsen, Niklas
name: Frigyesi, Attila
Publisher: name: figshare
Year: 2021
Subjects: Space Science
Cell Biology
Biotechnology
Information Systems not elsewhere classified
Mathematical Sciences not elsewhere classified
Cancer

description: Additional file 6: Table 1D. Day 3–72 hours of ICU treatment.