title: ÐÐµÐ³ÑÐ°Ð´Ð°ÑÐ¸Ñ Ð¸ ÑÐ¸Ð½ÑÐµÐ· de novo ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° ÑÐ¿Ð¸Ð´ÐµÑÐ¼Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÐ°ÐºÑÐ¾ÑÐ° ÑÐ¾ÑÑÐ° (ÑÐ­Ð¤Ð ) Ð² ÑÑÐ²Ð¾Ð»Ð¾Ð²ÑÑ Ð¸ Ð¾Ð¿ÑÑ Ð¾Ð»ÐµÐ²ÑÑ ÐºÐ»ÐµÑÐºÐ°Ñ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Degradation and de novo synthesis of the epidermal growth factor receptor (EGFR) in human stem and tumor cells
Creators: name: ÐÐ¾ÑÐ¾Ð², ÐÐ¸ÑÐ°Ð¸Ð»
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: ÑÐµÑÐµÐ¿ÑÐ¾Ñ Ð­Ð¤Ð
Ð´ÐµÐ³ÑÐ°Ð´Ð°ÑÐ¸Ñ
ÑÐ¸Ð½ÑÐµÐ· de novo
Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾ÑÐ½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð·
EGF receptor
degradation
de novo synthesis
inhibitory analysis

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¿Ð¾Ð¿ÑÑÐºÐµ Ð¿Ð¾Ð½ÑÑÑ Ð¿ÑÐ¸ÑÐ¸Ð½Ñ ÑÐ°Ð·Ð»Ð¸ÑÐ¸Ð¹ Ð² Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐµ Ð²Ð¾ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¸Ñ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð° Ð±ÐµÐ»ÐºÐ° ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° Ð­Ð¤Ð  Ð² HeLa Ð¸ ÑÐ½ÐÐ¡Ð Ð¿Ð¾ÑÐ»Ðµ Ð¿ÑÐµÐ´Ð²Ð°ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÑÐ¸Ð¼ÑÐ»ÑÑÐ¸Ð¸ Ð´ÐµÐ³ÑÐ°Ð´Ð°ÑÐ¸Ð¸ ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° Ð»Ð¸Ð³Ð°Ð½Ð´Ð¾Ð¼ Ð­Ð¤Ð . ÐÐ±ÑÐµÐºÑÐ¾Ð¼ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐ²Ð»ÑÐµÑÑÑ Ð¾Ð´Ð½Ð° Ð¾Ð¿ÑÑÐ¾Ð»ÐµÐ²Ð°Ñ (Ð¿Ð°ÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ°Ñ) Ð»Ð¸Ð½Ð¸Ñ ÐºÐ»ÐµÑÐ¾Ðº HeLa Ð¸ Ð¾Ð´Ð½Ð° Ð»Ð¸Ð½Ð¸Ñ ÐºÐ»ÐµÑÐ¾Ðº ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°, Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½Ð°Ñ Ð¸Ð· Ð´ÐµÑÐºÐ²Ð°Ð¼Ð¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ ÑÐ½Ð´Ð¾Ð¼ÐµÑÑÐ¸Ñ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°. ÐÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð¾ ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ½ÑÑ Ð±ÐµÐ»ÐºÐ¾Ð² Ð¾ÑÐµÐ½Ð¸Ð²Ð°Ð»Ð¸ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐ°Ð·Ð´ÐµÐ»ÐµÐ½Ð¸Ñ Ñ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÑÐ¸Ð¼ Ð¸Ð¼Ð¼ÑÐ½Ð¾Ð±Ð»Ð¾ÑÑÐ¸Ð½Ð³Ð¾Ð¼. ÐÑÐµÐ½ÐºÐ° ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð° Ð¼Ð ÐÐ Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»Ð¾ÑÑ ÐÐ¦Ð  Ð² ÑÐµÐ°Ð»ÑÐ½Ð¾Ð¼ Ð²ÑÐµÐ¼ÐµÐ½Ð¸. Ð¦ÐµÐ»ÑÑ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÐµÑÑÑ ÑÑÐ°Ð²Ð½Ð¸ÑÑ ÑÐ¾Ð»Ñ Ð»Ð¸Ð·Ð¾ÑÐ¾Ð¼Ð½Ð¾Ð¹ Ð´ÐµÐ³ÑÐ°Ð´Ð°ÑÐ¸Ð¸ Ð¸ ÑÐ¸Ð½ÑÐµÐ·Ð° de novo Ð² ÑÐµÐ³ÑÐ»ÑÑÐ¸Ð¸ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð° ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° Ð­Ð¤Ð  Ð² ÑÐ½ÐÐ¡Ð Ð¸ HeLa. ÐÐ¾ÐºÐ°Ð·Ð°Ð»Ð¸, ÑÑÐ¾ ÑÐ½ÐÐ¡Ð ÑÐµÐ»Ð¾Ð²ÐµÐºÐ° ÑÐºÑÐ¿ÑÐµÑÑÐ¸ÑÑÑÑ Ð±ÐµÐ»Ð¾Ðº ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° Ð­Ð¤Ð  Ð½Ð° ÑÑÐ¾Ð²Ð½Ðµ Ð½Ðµ Ð¼ÐµÐ½ÑÑÐµ, ÑÐµÐ¼ ÐºÐ»ÐµÑÐºÐ¸ Ð¾Ð¿ÑÑÐ¾Ð»ÐµÐ³Ð¾ ÑÐ¿Ð¸ÑÐµÐ»Ð¸Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð¿ÑÐ¾Ð¸ÑÑÐ¾Ð¶Ð´ÐµÐ½Ð¸Ñ HeLa, ÑÐ¾ÑÑÐ°Ð½ÑÑ Ð¿ÑÐ¸ ÑÑÐ¾Ð¼ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð° Ð½Ð¾ÑÐ¼Ð°Ð»ÑÐ½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ ÑÐ²Ð»ÑÑÑÑÑ ÑÐ°ÑÑÑÑ ÑÑÐ°Ð²Ð½Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð­Ð¤Ð -ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ½Ð¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ñ Ð²Ð°Ð¶Ð½Ð¾Ð³Ð¾ ÐºÐ°Ðº Ð´Ð»Ñ Ð¿Ð¾Ð½Ð¸Ð¼Ð°Ð½Ð¸Ñ Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð½Ð¾ÑÐ¼Ð°Ð»ÑÐ½ÑÑ ÑÐ¸Ð³Ð½Ð°Ð»ÑÐ½ÑÑ Ð¿ÑÑÐµÐ¹, ÑÐ°Ðº Ð¸ Ð´Ð»Ñ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð¾Ð² Ð²Ð¾Ð·Ð½Ð¸ÐºÐ½Ð¾Ð²ÐµÐ½Ð¸Ñ Ð¿Ð°ÑÐ¾Ð»Ð¾Ð³Ð¸Ð¹. Ð¡Ð½Ð¸Ð¶ÐµÐ½Ð¸Ðµ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð° ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ° Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ Ð¿Ð¾Ð´Ð°Ð²Ð»ÐµÐ½Ð¸Ñ ÑÑÐ°Ð½ÑÐ»ÑÑÐ¸Ð¸ Ð² Ð¾Ð±ÐµÐ¸Ñ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð»Ð¸Ð½Ð¸ÑÑ ÑÑÐ¾Ð¶Ðµ Ð¸ ÑÐ²Ð¸Ð´ÐµÑÐµÐ»ÑÑÑÐ²ÑÐµÑ Ð¾ Ð½Ð°Ð»Ð¸ÑÐ¸Ð¸ ÑÐ¸Ð½ÑÐµÐ·Ð° de novo Ð² Ð¾Ð±ÐµÐ¸Ñ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð»Ð¸Ð½Ð¸ÑÑ. Ð¡ÑÐ¸Ð¼ÑÐ»ÑÑÐ¸Ñ Ð´ÐµÐ³ÑÐ°Ð´Ð°ÑÐ¸Ð¸ ÑÐ­Ð¤Ð  Ð¿ÑÐ¸ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ð¸ Ð»Ð¸Ð³Ð°Ð½Ð´Ð° Ð² Ð¿ÑÐ¸ÑÑÑÑÑÐ²Ð¸Ð¸ ÑÐ¸ÐºÐ»Ð¾Ð³ÐµÐºÑÐ¸Ð¼Ð¸Ð´Ð° Ð¿ÑÐ¸Ð²Ð¾Ð´Ð¸Ñ Ðº Ð¿ÑÐ°ÐºÑÐ¸ÑÐµÑÐºÐ¸ Ð¿Ð¾Ð»Ð½Ð¾Ð¼Ñ Ð¸ÑÑÐµÐ·Ð½Ð¾Ð²ÐµÐ½Ð¸Ñ ÑÐ­Ð¤Ð  ÐºÐ¾ Ð²ÑÐ¾ÑÑÐ¼ ÑÑÑÐºÐ°Ð¼.
description: This work is devoted to an attempt to understand the reasons for the differences in the dynamics of restoration of the amount of EGF receptor protein in HeLa and enMSCs after preliminary stimulation of receptor degradation by EGF ligand. The object of the study is one tumor (pathological) HeLa cell line and one human cell line isolated from desquamated human endometrium. The amount of receptor proteins was assessed by electrophoretic separation followed by immunoblotting. The amount of mRNA was assessed by real-time PCR. The aim of the work is to compare the role of lysosomal degradation and de novo synthesis in the regulation of the amount of EGF receptor in enMSCs and HeLa. It was shown that human enMSCs express the EGF receptor protein at a level no less than HeLa cells of tumor epithelial origin, while maintaining the properties of normal cells. The results obtained are part of a comparative analysis of the EGF-receptor system, which is important both for understanding the organization of normal signaling pathways and for the mechanisms of the occurrence of pathologies. The decrease in the amount of receptor under conditions of translation suppression in both cell lines is similar and indicates the presence of de novo synthesis in both cell lines. Stimulation of rEGF degradation by the action of a ligand in the presence of cycloheximide leads to the almost complete disappearance of rEGF by the second day.