Data from: Novel microsatellite markers for epiphytic bromeliad Tillandsia recurvata L., in an urban landscape in South-eastern Brazil
Creators: name: Quail, Megan
name: Ramos, Flavio
name: Dallimore, Thom
name: Provan, Jim
name: Ashton, Paul
name: Clayton-Brown, Jennifer
name: Batke, Sven
Publisher: name: Zenodo
Year: 2023
Subjects: Tillandsia recurvata
Population genetics
urbanisation
microsatellites

description: The authors present seven novel microsatellite markers for <em>Tillandsia recurvata</em> L. The genome assemble sequences of <em>T. recurvata</em> were obtained from NCBI (7.2Gb) (Sayers <em>et al</em>. 2022). The BioProject Accession and accession numbers are PRJNA701548 and SRX10089449, respectively. The microsatellite identification software Krait (0.5.2) (Du <em>et al</em>. 2018) was used to detect suitable microsatellites, both genome-wide and in the noncoding regions of <em>T. recurvata</em>. The single sequence repeats (SSRs) were refined to a minimum number of seven repeats of di-, tri-, or tetra-nucleotide repeat motifs. These sequences were further limited to SSRs of more than 100bp in length and low GC content (&lt;50%). Krait (0.5.2) (Du <em>et al</em>. 2018) was also used to design the primers for the selected SSR sequences, in conjunction with the integrated Primer3 software. The criteria for primer selection included: a primer length of 18-26bp, an optimal melting temperature (Tm) of 54-59°C and GC content of &lt;50%. These designed primers were single-plexed and amplified using the following PCR cycle: initial denaturation (95°C for 3 min), 34 cycles of 95°C for 30s, annealing for 30s (JP01-JP12: 54°C, 4873TD + 35251TD: 56°C, 19286TD: 53°C, 5044TD + 186664TD + 214633TD: 58°C), 72°C for 1 min and a final extension of 72°C for 5 mins. The authors make this information available to other researchers, to continue the investigation of epiphyte genetics.
description: The corresponding author that designed each microsatellite marker has been indicated in the reference column of the dataset. The authors invite any correspondence and queries relating to these microsatellite markers. Funding provided by: Edge Hill University<br>Crossref Funder Registry ID: http://dx.doi.org/10.13039/100010009<br>Award Number: Funding provided by: Institute from Federal University of Alfenas*<br>Crossref Funder Registry ID: <br>Award Number: Funding provided by: Conselho Nacional de Desenvolvimento Científico e Tecnológico<br>Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100003593<br>Award Number: (306796/2020-1)
description: The genome assemble sequences of <em>T. recurvata</em> were obtained from NCBI (7.2Gb) (Sayers <em>et al</em>. 2022). The BioProject Accession and accession numbers are PRJNA701548 and SRX10089449, respectively. The microsatellite identification software Krait (0.5.2) (Du<em> et al</em>. 2018) was used to detect suitable microsatellites, both genome-wide and in the noncoding regions of <em>T. recurvata</em>. The single sequence repeats (SSRs) were refined to a minimum number of seven repeats of di-, tri-, or tetra-nucleotide repeat motifs. These sequences were further limited to SSRs of more than 100bp in length and low GC content (&lt;50%). Krait (0.5.2) (Du <em>et al</em>. 2018) was also used to design the primers for the selected SSR sequences, in conjunction with the integrated Primer3 software. The criteria for primer selection included: a primer length of 18-26bp, an optimal melting temperature (Tm) of 54-59°C and GC content of &lt;50%. These designed primers were single-plexed and amplified using the following PCR cycle: initial denaturation (95°C for 3 min), 34 cycles of 95°C for 30s, annealing for 30s (JP01-JP12: 54°C, 4873TD + 35251TD: 56°C, 19286TD: 53°C, 5044TD + 186664TD + 214633TD: 58°C), 72°C for 1 min and a final extension of 72°C for 5 mins.