Additional file 1 of Impact of an oligosaccharide-based polymer on the metabolic profiles and microbial ecology of weanling pigs experimentally infected with a pathogenic E. coli
Creators: name: Kim, Kwangwook
name: Jinno, Cynthia
name: Li, Xunde
name: Bravo, David
name: Cox, Eric
name: Ji, Peng
name: Liu, Yanhong
Publisher: name: figshare
Year: 2024
Subjects: subject: Space Science
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Biotechnology
subject: Environmental Sciences not elsewhere classified
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Virology

description: Additional file 1: Fig. S1. Partial least squares discriminant analysis (PLS-DA) 2D score plot of the metabolites in serum showed separated clusters between the CON and CAR groups on d 5 (A) and d 11 post-inoculation (B). Partial least squares discriminant analysis (PLS-DA) 2D score plot of the metabolites in distal colon digesta showed separated clusters between the CON and 10 or 20 mg/kg oligosaccharide-based polymer active substance groups on d 11 post-inoculation (C). CON = Basal nursery diet (control). LOW = Control diet supplemented with 10 mg/kg of oligosaccharide-based polymer. HIGH = Control diet supplemented with 20 mg/kg of oligosaccharide-based polymer. CAR = Control diet supplemented with 50 mg/kg carbadox. Shaded areas in different colors represent in 95% confidence interval. Fig. S2. Significantly changed pathways (−log10(P) &gt; 1.5) in serum between the control and 50 mg/kg carbadox on d 5 (A) or d 11 post-inoculation (C). The x-axis represents the pathway impact values and the y-axis represents the −log(P) values from the pathway enrichment analysis. Metabolite set enrichment analysis (B and D) shows the metabolic pathways were altered in control compared to 50 mg/kg carbadox on d 5 or d 11 post-inoculation, respectively. Both pathway analysis and metabolite set enrichment analysis were performed using identified metabolites with VIP &gt; 1. Fig. S3. Alpha diversity as indicated by Shannon (A) and Chao 1 (B) in distal colon digesta of enterotoxigenic E. coli F18 challenged pigs fed diets supplemented with different dose of oligosaccharide-based polymer active substance or antibiotics (carbadox) on d 5 and 11 post-inoculation. a–cMeans without a common superscript are different (P &lt; 0.05). Each least squares mean represents 6 observations. CON = Basal nursery diet (control); LOW = Control diet supplemented with 10 mg/kg oligosaccharide-based polymer active substance; HIGH = Control diet supplemented with 20 mg/kg oligosaccharide-based polymer active substance; CAR = Control diet supplemented with 50 mg/kg carbadox. Fig. S4. Stacked bar plot showing the relative abundance of bacterial phyla in colon digesta of enterotoxigenic E. coli F18 challenged pigs fed diets supplemented with different dose of oligosaccharide-based polymer active substance or antibiotics (carbadox) on d 5 and 11 post-inoculation (A). Violin plot showing the relative abundance of individual bacterial phylum (B). a,bMeans without a common superscript are different (P &lt; 0.05). Each least squares mean represents 6 observations. CON = Basal nursery diet (control); LOW = Control diet supplemented with 10 mg/kg oligosaccharide-based polymer active substance; HIGH = Control diet supplemented with 20 mg/kg oligosaccharide-based polymer active substance; CAR = Control diet supplemented with 50 mg/kg carbadox.