title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ AgNPs Ð½Ð° ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÐµ Ð·Ð²ÐµÐ½ÑÑ ÑÐ¸ÑÑÐµÐ¼Ñ, Ð·Ð°ÑÐ¸ÑÐ°ÑÑÐµÐ¹ Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼ Ð¾Ñ Ð³ÑÐ¸Ð¿Ð¿Ð¾Ð·Ð½Ð¾Ð¹ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: The influence of AgNPs on various parts of the system that protects the body from influenza infection
Creators: name: Ð¢ÐºÐ°ÑÐµÐ², ÐÑÑÑÐ¼
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸ÑÑ ÑÐµÑÐµÐ±ÑÐ°
ÑÐµÑÑÐ»Ð¾Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð½
Ð¸Ð½ÑÐµÑÐ»ÐµÐ¹ÐºÐ¸Ð½Ñ
Ð»ÐµÐ¹ÐºÐ¾ÑÐ¸ÑÑ
Ð¾ÐºÑÐ¸Ð´Ð°Ð·Ð½Ð°Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ
Ð²Ð¸ÑÑÑÐ½Ð°Ñ Ð¸Ð½ÑÐµÐºÑÐ¸Ñ
Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¼ Ð¼ÐµÐ´Ð¸
silver nanoparticles
ceruloplasmin
interleukin
leukocites
oxidase activity
infection
copper

description: ÐÐ·Ð²ÐµÑÑÐ½Ð¾, ÑÑÐ¾ Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸ÑÑ ÑÐµÑÐµÐ±ÑÐ° (AgNPs) Ð¾Ð±Ð»Ð°Ð´Ð°ÑÑ Ð¿ÑÐ¾ÑÐ¸Ð²Ð¾Ð²Ð¸ÑÑÑÐ½ÑÐ¼ ÑÑÑÐµÐºÑÐ¾Ð¼, Ð½Ð¾ ÑÐ¿Ð¾ÑÐ¾Ð±Ñ Ð²Ð¾Ð·Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ AgNPsÐ½Ð° ÑÐ¸ÑÑÐµÐ¼Ñ, Ð·Ð°ÑÐ¸ÑÐ°ÑÑÑÑ Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼ Ð¾Ñ Ð²Ð¸ÑÑÑÐ° Ð³ÑÐ¸Ð¿Ð¿Ð° Ð¸Ð·ÑÑÐµÐ½Ñ Ð½ÐµÐ´Ð¾ÑÑÐ°ÑÐ¾ÑÐ½Ð¾. ÐÐ¾ÑÑÐ¾Ð¼Ñ ÑÐµÐ»Ñ ÑÐ°Ð±Ð¾ÑÑ ÑÐ¾ÑÑÐ¾Ð¸Ñ Ð² Ð¸Ð·ÑÑÐµÐ½Ð¸Ð¸ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸ÑÑ ÑÐµÑÐµÐ±ÑÐ° Ð½Ð° Ð·Ð²ÐµÐ½ÑÑ ÑÐ¸ÑÑÐµÐ¼Ñ, Ð·Ð°ÑÐ¸ÑÐ°ÑÑÐµÐ¹ Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼ Ð¾Ñ Ð³ÑÐ¸Ð¿Ð¿Ð¾Ð·Ð½Ð¾Ð¹ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸. Ð ÑÐ°Ð±Ð¾ÑÐµ Ð¿Ð¾Ð´ÑÐ²ÐµÑÐ¶Ð´ÐµÐ½Ð¾, ÑÑÐ¾ AgNPs Ð²Ð»Ð¸ÑÑÑ Ð½Ð° Ð¾ÐºÑÐ¸Ð´Ð°Ð·Ð½ÑÑ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð±ÐµÐ»ÐºÐ° Ð¾ÑÑÑÐ¾Ð¹ ÑÐ°Ð·Ñ ÑÐµÑÑÐ»Ð¾Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð½Ð°, ÑÐ½Ð¸Ð¶Ð°Ñ ÐµÐ³Ð¾ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ Ðº Ð¾ÐºÐ¸ÑÐ»ÐµÐ½Ð¸Ñ Ð¶ÐµÐ»ÐµÐ·Ð°. ÐÐ°Ð²Ð¸ÑÐ¸Ð¼Ð¾ÑÑÑ ÑÑÐ¾Ð²Ð½Ñ Ð¸Ð½ÑÐµÑÐ»ÐµÐ¹ÐºÐ¸Ð½Ð¾Ð² 2 Ð¸ 10 Ð¾Ñ Ð½Ð°Ð»Ð¸ÑÐ¸Ñ Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸Ñ Ð² Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ðµ Ð½Ðµ Ð±ÑÐ»Ð° Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð° Ð² ÑÐ¾Ð´Ðµ Ð½Ð°ÑÐµÐ³Ð¾ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°, Ð½Ð¾ ÑÑÐ¾Ð²ÐµÐ½Ñ Ð¸Ð½ÑÐµÑÐ»ÐµÐ¹ÐºÐ¸Ð½Ð° 6 Ð¿ÑÐ¸ Ð½Ð°Ð»Ð¸ÑÐ¸Ð¸ Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸Ñ ÑÐµÑÐµÐ±ÑÐ° Ð² Ð¾ÑÐ³Ð°Ð½Ð¸Ð·Ð¼Ðµ Ð¿Ð¾Ð²ÑÑÐ°Ð»ÑÑ. ÐÐ»Ñ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ° Ñ Ð»ÐµÐ¹ÐºÐ¾ÑÐ¸ÑÐ°Ð¼Ð¸ Ð±ÑÐ» ÑÐ¿ÐµÑÐ¸Ð°Ð»ÑÐ½Ð¾ Ð¾ÑÐ¸ÑÐµÐ½ Ð±ÐµÐ»Ð¾Ðº ÑÐµÑÑÐ»Ð¾Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð½Ð° Ð² Ð´Ð²ÑÑ ÑÐ¾ÑÐ¼Ð°Ñ ÑÐ¾Ð»Ð¾-ÑÐ¾ÑÐ¼Ðµ Ð¸ ÑÐ¾ÑÐ¼Ðµ ÑÐµÑÑÐ»Ð¾Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð½Ð°, Ð² ÑÑÑÑÐºÑÑÑÐµ ÐºÐ¾ÑÐ¾ÑÐ¾Ð³Ð¾ ÑÐ¾Ð´ÐµÑÐ¶Ð¸ÑÑÑ Ð¸Ð¾Ð½Ñ ÑÐµÑÐµÐ±ÑÐ°. ÐÐ»Ñ Ð¾ÑÐ¸ÑÐµÐ½Ð¸Ñ Ð´Ð°Ð½Ð½ÑÑ Ð±ÐµÐ»ÐºÐ¾Ð² Ð±ÑÐ»Ð¾ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¾ 3 ÑÑÐ¾Ð¼Ð°ÑÐ¾Ð³ÑÐ°ÑÐ¸Ð¸ (Ð¸Ð¾Ð½Ð¾Ð¾Ð±Ð¼ÐµÐ½Ð½Ð°Ñ, Ð°ÑÑÐ¸Ð½Ð½Ð°Ñ Ð¸ ÑÐºÑÐºÐ»ÑÐ·Ð¸Ð¾Ð½Ð½Ð°Ñ) Ñ ÑÐµÐ»ÑÑ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ ÑÐ¸ÑÑÐ¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ°. Ð­ÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½Ñ Ñ Ð»ÐµÐ¹ÐºÐ¾ÑÐ¸ÑÐ°Ð¼Ð¸ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÑÐ» Ð¸Ð· ÑÐµÐ±Ñ Ð¸Ð½ÐºÑÐ±Ð°ÑÐ¸Ñ ÐºÐ»ÐµÑÐ¾Ðº Ð»ÐµÐ¹ÐºÐ¾ÑÐ¸ÑÐ¾Ð² Ñ ÑÐµÑÑÐ»Ð¾Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð½Ð¾Ð¼ Ð² Ð´Ð²ÑÑ Ð¾ÑÐ¸ÑÐµÐ½Ð½ÑÑ ÑÐ¾ÑÐ¼Ð°Ñ Ð´Ð»Ñ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¸Ñ Ð°Ð¿Ð¾Ð¿ÑÐ¾Ð·Ð° Ð´Ð°Ð½Ð½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº. ÐÐ¿Ð¾Ð¿ÑÐ¾Ð· Ð»ÐµÐ¹ÐºÐ¾ÑÐ¸ÑÐ¾Ð² Ð¿ÑÐ¸ Ð¸Ð½ÐºÑÐ±Ð°ÑÐ¸Ð¸ Ñ ÑÐµÑÑÐ»Ð¾Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð½Ð¾Ð¼ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸ÐµÐ¹ 1,5 Ð¼Ð³/Ð¼Ð» Ð½Ð°ÑÑÑÐ¿Ð¸Ð» ÑÐµÑÐµÐ· 2 ÑÐ°ÑÐ° Ð¿Ð¾ÑÐ»Ðµ Ð¸Ð½ÐºÑÐ±Ð°ÑÐ¸Ð¸, Ð¿ÑÐ¸ÑÐµÐ¼ Ð² Ð·Ð½Ð°ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ¿ÐµÐ½Ð¸ Ð°Ð¿Ð¾Ð¿ÑÐ¾Ð· Ð½Ð°Ð±Ð»ÑÐ´Ð°ÐµÑÑÑ Ð¿ÑÐ¸ Ð¸Ð½ÐºÑÐ±Ð°ÑÐ¸Ð¸ Ñ ÑÐ¾Ð»Ð¾-ÑÐ¾ÑÐ¼Ð¾Ð¹ ÑÐµÑÑÐ»Ð¾Ð¿Ð»Ð°Ð·Ð¼Ð¸Ð½Ð¾Ð¼, ÑÐµÐ¼ Ñ ÑÐ¾ÑÐ¼Ð¾Ð¹, ÑÐ¾Ð´ÐµÑÐ¶Ð°ÑÐµÐ¹ ÑÐµÑÐµÐ±ÑÐ¾.
description: It is known that silver nanoparticles have an antiviral effect, but the ways in which AgNPs affect the system that protects the body from the influenza virus have not been studied enough.Therefore, the aim of the work is to study the effect of silver nanoparticles on the links of the system that protects the body from influenza infection. The work confirmed that silver nanoparticles affect the oxidase activity of the acute phase protein ceruloplasmin, reducing its ability to oxidize iron.The dependence of the level of interleukins 2 and 10 on the presence of nanoparticles in the body was not found during our experiment, but the level of interleukin 6 increased in the presence of silver nanoparticles in the body. For the experiment with leukocytes, the ceruloplasmin protein was specially purified in two forms - the holo-form and the ceruloplasmin form, the structure of which contains silver ions.To purify these proteins, 3 chromatography (ion exchange, affinity, and gel filtration) were carried out in order to obtain the purest protein.The leukocyte experiment was the incubation of leukocyte cells with ceruloplasmin in two purified forms to detect apoptosis of these cells. Apoptosis of leukocytes during incubation with ceruloplasmin at a concentration of 1.5 mg/ml occurred 2 hours after incubation, and to a large extent apoptosis is observed during incubation with the holo-form ceruloplasmin than with the form containing silver