cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
The role of WT1 in Kras-driven lung cancer
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Lung cancer is the leading cause of cancer death among both men and women in the US and more than 160,000 lung cancer deaths are expected in 2008. The two main types of lung cancer are small cell lung cancer and non-small cell lung cancer, which comprise 20% and 80% of the lung cancer cases respectively. Lung cancer is a cancer that forms in tissues of the lung, usually in the cells lining air passages. Tobacco exposure and other environmental agents can damage cells in ways that set the cancer process in motion. This damage can be irreversible, leading towards the final development of lung cancer. A specific set of proto-oncogenes can be affected in those cells within the lungs, among Kras is represented. Activation of oncogenic properties of Kras by cell damage can trigger a signaling program that includes a large set of genes. Promotion of this program can be responsible for the final progression of lung cancer.

 The aim of this proposal is to study the role of one of these signaling genes, Wt1, in Kras-driven lung cancer. Preliminary results in our lab pointed at Wt1 as a potential regulator of oncogenic Kras effects in lung cancer. We hypothesize that Wt1 may be regulating critical genes that mediate the progression of lung cancers harboring Kras mutations. To address this question, we will focus on several aims. We will first make use of functional in vitro and in vivo experiments using mouse and human lung cancer cell lines with defined Kras mutations to test the implication of Wt1 and signaling components in lung cancer cells. In addition, we will take advantage of mouse genetics to study the role of Wt1 loss in a mouse model of Kras-driven lung tumorigenesis lacking Wt1 expression.

 We anticipate that attenuation of Wt1 or related signaling components might have a significant impact on the growth of lung tumors. We plan to transfer the knowledge gained by these experiments to test the importance of Wt1 and other related signaling components in human samples. We expect that some of the genes being part of the presented proposal will have a critical role in human lung cancer patients. Therefore, we hope that the results coming from this study will set the rationale for the development of new therapies that could significantly increase the overall survival of patients with this fatal disease.
Drosophila metzii Sturtevant 1921
Creators: name: Sinclair, Bradley J.
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Drosophilidae
Drosophila
Drosophila metzii

description: metzii Sturtevant
Drosophila metzii Sturtevant, 1921: 78.
Drosophila (Drosophila) metzii: Carson et al. 1983: 241 [records]; Brake & Bächli 2008: 69 [catalogue].
Distribution. Introduced. Neotropical; Galápagos: Isabela, Santa Cruz.
Remarks. Adults of this species were found in the highlands (Carson et al. 1983).
description: Published as part of Sinclair, Bradley J., 2023, An annotated checklist of the Diptera of the Galápagos Archipelago (Ecuador), pp. 1-102 in Zootaxa 5283 (1) on pages 55-56, DOI: 10.11646/zootaxa.5283.1.1, http://zenodo.org/record/7912667
Henosepilachna urvillei Montrouzier
Creators: name: Nattier, Romain
name: Jourdan, Hervé
name: Mille, Christian
name: Chazeau, Jean
Publisher: name: Zenodo
Year: 2015
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Coleoptera
Coccinellidae
Henosepilachna
Henosepilachna urvillei

description: Henosepilachna urvillei (Montrouzier)
Epilachna urvillei Montrouzier, 1861: 305.
Epilachna moultoni Crotch, 1874: 89 —synonymized by Korschefsky 1933: 236. Henosepilachna urvillei: Bielawski 1972: 559.
This species was described from Lifou (type specimen) by Montrouzier. Bielawski (1963) added details to the original description. This ladybug is widespread on Grande Terre, Loyalty Islands and Isle of Pines (Chazeau, pers. obs.). It appears widely distributed in Melanesia, occurring in Papua New Guinea, Solomon and Fiji islands. Li (1993) stated that only one specimen is recorded from Australia, where this species may not be established.
description: Published as part of Nattier, Romain, Jourdan, Hervé, Mille, Christian & Chazeau, Jean, 2015, An annotated checklist of the Coccinellidae (Coleoptera) from New Caledonia, pp. 301-331 in Zootaxa 4058 (3) on page 316, DOI: 10.11646/zootaxa.4058.3.1, http://zenodo.org/record/236980
Additional file 1 of EGF-like growth factors upregulate pentraxin 3 expression in human granulosa-lutein cells
Creators: name: Li, Yuxi
name: Chang, Hsun-Ming
name: Zhu, Hua
name: Sun, Ying-Pu
name: Leung, Peter C. K.
Publisher: name: figshare
Year: 2024
Subjects: subject: Biochemistry
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Physiology
subject: Chemical Sciences not elsewhere classified
subject: Immunology
subject: FOS: Clinical medicine
subject: Developmental Biology
subject: Cancer
subject: Hematology
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Computational Biology

description: Supplementary Material 1
Drosophila repleta Wollaston 1858
Creators: name: Sinclair, Bradley J.
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Drosophilidae
Drosophila
Drosophila repleta

description: repleta Wollaston
Drosophila repleta Wollaston, 1858: 117
Drosophila (Drosophila) repleta: Carson et al. 1983: 241 [records]; Peck et al. 1998: 228 [introduced species]; Causton et al. 2006: 134 [introduced species].
Distribution. Introduced. Cosmopolitan; Galápagos: Santa Cruz.
Remarks. Adults of this species were found in the lowland zones (Carson et al. 1983).
description: Published as part of Sinclair, Bradley J., 2023, An annotated checklist of the Diptera of the Galápagos Archipelago (Ecuador), pp. 1-102 in Zootaxa 5283 (1) on page 56, DOI: 10.11646/zootaxa.5283.1.1, http://zenodo.org/record/7912667
Patterns of Tobacco and Marijuana Use Among Young Adults
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Nationally (38.4%) and in California (30.4%), young adults aged 18 to 25 report the highest rate of current cigarette use, and constitute the largest segment of tobacco marketing efforts relative to other age groups. Thus, young adults are an important population to target for understanding risk factors of use and designing effective programs to stop smoking. Marijuana (MJ) use, a risk factor for smoking cigarettes, may help to explain why some young adults have been a challenging population to treat through smoking cessation interventions. The overall goal of the proposed project is to understand how MJ use affects cigarette use and thoughts about use. This information will inform the development of an intervention to treat cigarette and MJ use among young adults.

 The primary aims of the proposed project are: 1) To characterize the cigarette use patterns and cessation attempts in young adult smokers who concurrently use MJ (CIG/MJ) compared to those who do not (CIG-only); and 2) To examine differences in smoking-related thoughts between the CIG/MJ and CIG-only groups. We will administer an online survey to at least 1500 young adults recruited through the Internet (e.g., social networking sites, search engines). Young adults who are eligible (age 18-25, read English, currently smoke cigarettes) and give informed consent will complete a 20 minute survey. Those who complete the survey will have a chance to win a raffle prize. We will assess demographic characteristics, cigarette, alcohol, MJ and other drug use, attempts participants have made to quit using cigarettes and MJ, cigarette and MJ dependence symptoms, motivation to quit using cigarettes and MJ, confidence around quitting cigarette and MJ use, expectations about the effects of cigarette and MJ use, and expectations about the use of both cigarettes and MJ simultaneously. Analyses will compare young adult cigarette and MJ users (CIG/MJ) to those who use cigarettes only (CIG-only). Hypotheses are that the CIG/MJ group will: 1a) smoke cigarettes more heavily than the CIG-only group; 1b) be more dependent on cigarettes than the CIG-only group; 2a) have had fewer attempts to quit smoking cigarettes than those in the CIG-only group; 2b) have had shorter attempts to quit smoking cigarettes than those in the CIG-only group; 3) have lower motivation to quit smoking cigarettes than the CIG-only group; and 4) report more positive expectations about the effects of cigarettes than the CIG-only group. Secondary aims are to examine patterns of cigarette and MJ use among groups of people (e.g., gender, ethnicity, where they live) and how cigarettes and MJ are used together. This study will provide valuable information that can be used to develop interventions targeted to reduce smoking behavior in young adults throughout California and the United States.
Drosophila ananassae Doleschall 1858
Creators: name: Sinclair, Bradley J.
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Drosophilidae
Drosophila
Drosophila ananassae

description: ananassae Doleschall
Drosophila ananassae Doleschall, 1858: 128.
Drosophila (Sophophora) ananassae: Carson et al. 1983: 241 [records]; Peck et al. 1998: 228 [introduced species]; Causton et al. 2006: 134 [introduced species].
Distribution. Introduced. Cosmopolitan; Galápagos: Santa Cruz.
Remarks. This species occurs in the lowland zones (Carson et al. 1983).
description: Published as part of Sinclair, Bradley J., 2023, An annotated checklist of the Diptera of the Galápagos Archipelago (Ecuador), pp. 1-102 in Zootaxa 5283 (1) on page 56, DOI: 10.11646/zootaxa.5283.1.1, http://zenodo.org/record/7912667
Additional file 1: Figure S1. of Combined platelet-rich plasma and lipofilling treatment provides great improvement in facial skin-induced lesion regeneration for scleroderma patients
Creators: name: VirzĂŹ, Francesco
name: Bianca, Paola
name: Giammona, Alessandro
name: Apuzzo, Tiziana
name: Franco, Simone Di
name: Mangiapane, Laura
name: Colorito, Maria
name: Catalano, Dario
name: Scavo, Emanuela
name: Nicotra, Annalisa
name: Benfante, Antonina
name: Pistone, Giuseppe
name: Caputo, Valentina
name: Dieli, Francesco
name: Pirrello, Roberto
name: Stassi, Giorgio
Publisher: name: Figshare
Year: 2017
Subjects: subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Physiology
subject: Ecology
subject: Immunology
subject: FOS: Clinical medicine
subject: Developmental Biology
subject: Hematology

description: showing AD-MSCs from both healthy subjects and SSc patients with increased levels of CD271 expression. Representative dot plot showing the expression of CD271 corresponding isotype match control in freshly isolated SVF and long-term propagated AD-MSCs from healthy subjects (upper panels) and SSc patients (lower panels), performed by flow cytometry. (PDF 205Â kb)
Protein networks for nicotinic acetylcholine receptors
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Cigarette smoking delivers nicotine to the brain where it binds to specific proteins in the membrane of cells. These proteins are called nicotinic acetylcholine receptors (nAChRs), and their activation by nicotine is the first molecular step in the addiction process. Normally these receptors are activated by a chemical in the brain called acetylcholine. Activation of the receptors sets in motion a chain of events in the cell that depends on the other proteins likely to be tethered in close proximity to the receptor. Depending on the composition of those partners, receptor activation can have a variety of effects. In fact, activation of nAChRs normally contributes to numerous higher order brain functions and behaviors. Aberrant nAChR activation has been implicated in a number of diseases including Alzheimer’s, Parkinson’s, and schizophrenia, in addition to nicotine addiction.

 We have obtained preliminary results revealing the identity of three proteins that are candidate partners for a major nAChR in brain. In addition to its abundance, the nAChR subtype involved is interesting because it is exceptional at mobilizing calcium to set in motion numerous events in brain cells and in the circuits they comprise. These three candidates partners are (1) PSD-95, a protein that can act as a scaffold to assemble components in cells, (2) PMCA2 which acts as a calcium pump to remove excess calcium from cells, and (3) NCAM2 which is a novel member of a family of proteins that connect cells together (cell adhesion molecules). Given the known properties of these proteins and given our preliminary results suggesting they are associated with the same nAChR, the three candidates may work together to dictate the outcome of receptor activation.

 Three Specific Aims are proposed here. The First Aim is designed to understand how these three proteins interact with nAChRs, specifically testing whether PSD-95 controls the location of the receptor on the cell surface because this can be critical in determining when the receptor is activated. The Second Aim uses functional assays to study how interactions of the three proteins with the receptor and with each other determine the consequences of nAChR activation. The Third Aim determines how NCAM2 affects nAChR location, nAChR linkage to other components both inside the cell and outside the cell, and nAChR function.

 The information obtained here will provide new insight into molecular mechanisms that determine the consequences of nicotinic stimulation. These mechanisms represent targets that are vulnerable to tobacco-derived nicotine. Understanding their interactions and physiological consequences will not only increase our knowledge of nicotinic physiology in brain, but it will also underscore the dangers of nicotine consumption and may suggest therapeutic strategies that can be tested.
Rodolia cardinalis Mulsant
Creators: name: Nattier, Romain
name: Jourdan, Hervé
name: Mille, Christian
name: Chazeau, Jean
Publisher: name: Zenodo
Year: 2015
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Coleoptera
Coccinellidae
Rodolia
Rodolia cardinalis

description: Rodolia cardinalis (Mulsant)
Vedalia cardinalis Mulsant, 1850: 906.
Novius cardinalis: Crotch 1874: 283.
Eurodolia cardinalis: Weise 1895: 150.
Rodolia (Macronovius) cardinalis: Weise 1905: 220. Macronovius cardinalis: Weise 1922: 104. Rodolia cardinalis: Korschefsky 1931: 101.
This species was originally described from Australia. It is considered to be a recent introduction in New Caledonia and Cook Islands. It has been introduced successfully into Vanuatu, Fiji Islands, Micronesia (Guam), Hawaii, in the southern United States (California, Florida, Louisiana, South Carolina and Texas), southern Europe, North Africa, South Africa, India and Indonesia (Java). It was also accidentally introduced to New Zealand, where it is not widespread. The date of arrival in New Caledonia is unknown, but it probably dispersed from Vanuatu (introduced to there in 1964 by Cochereau from Fiji Islands). It has been regularly collected since its first reported occurrence in 1975 in Nouméa but remains uncommon. This species is a major biocontrol agent worldwide as it represents the world’s first biocontrol success after its introduction in California in citrus orchards to control Icerya purchasi Maskell outbreaks (Debach 1974). This is an aggressive predator against scale insects, especially Icerya spp. (Hemiptera, Monophlebidae). In Nouméa, it has been seen feeding on I. seychellarum (Westwood).
Material examined. NEW CALEDONIA: Nouméa, Anse Vata, 11.vii.1975 (J. Gutierrez rec.) (ONNC); Nouméa, 28.ix.1977 (J. Fons rec.) (ONNC); Nouméa, Anse Vata, ix.1978 (J. Chazeau rec.) (ONNC).
description: Published as part of Nattier, Romain, Jourdan, Hervé, Mille, Christian & Chazeau, Jean, 2015, An annotated checklist of the Coccinellidae (Coleoptera) from New Caledonia, pp. 301-331 in Zootaxa 4058 (3) on page 316, DOI: 10.11646/zootaxa.4058.3.1, http://zenodo.org/record/236980
Drosophila malerkotliana Parshad & Paika 1965
Creators: name: Sinclair, Bradley J.
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Drosophilidae
Drosophila
Drosophila malerkotliana

description: malerkotliana Parshad & Paika (M. Parchami-Araghi pers. comm.).
Drosophila malerkotliana Parshad & Paika, 1965: 235.
Distribution. Introduced. Afrotropical, Neotropical, Oriental; Galápagos: Floreana, Isabela, San Cristóbal, Santa Cruz.
Remarks. Adults of this species were recently reared from various fruits throughout the main populated islands (Lehnen et al. unpubl.). This species is very similar morphologically to D. ananassae.
description: Published as part of Sinclair, Bradley J., 2023, An annotated checklist of the Diptera of the Galápagos Archipelago (Ecuador), pp. 1-102 in Zootaxa 5283 (1) on page 56, DOI: 10.11646/zootaxa.5283.1.1, http://zenodo.org/record/7912667
Rodolia delobeli Chazeau
Creators: name: Nattier, Romain
name: Jourdan, Hervé
name: Mille, Christian
name: Chazeau, Jean
Publisher: name: Zenodo
Year: 2015
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Coleoptera
Coccinellidae
Rodolia
Rodolia delobeli

description: Rodolia delobeli Chazeau
Rodolia delobeli Chazeau, 1981: 57.
This endemic species is only known from southern New Caledonia (Ouen-Toro, sclerophyllous forest, dense rain forest and ultramafic shrubs in Rivière Bleue, dense rain forest in La Thi Valley). It is an uncommon species and no information is available on its prey preferences.
Material examined. NEW CALEDONIA: Nouméa, Ouen Toro, 20.x.1977 (J. Chazeau rec.) (ONNC); Nouméa, Ouen Toro, 24.vi.1978 (A. Delobel rec.) (ONNC); Forêt de la Thi, 27.iv.1981 (J. Chazeau rec.) (ONNC); Rivière Bleue (parc.7), forêt dense humide sur pente, 170m, 420. xii.1985, 825. xii.1986 (L. Bonnet de Larbogne & J. Chazeau rec.) (ONNC); Rivière Bleue, maquis sur crête, 310m, 25.xii. 19865. i.1987, 5.20.i, 2031. i.1987 (L. Bonnet de Larbogne, J. Chazeau, A.& S. Tillier rec.) (ONNC); Pindaï, 9.xi.1982, forêt sclérophylle (E. & M. Schlinger rec.) (ONNC).
description: Published as part of Nattier, Romain, Jourdan, Hervé, Mille, Christian & Chazeau, Jean, 2015, An annotated checklist of the Coccinellidae (Coleoptera) from New Caledonia, pp. 301-331 in Zootaxa 4058 (3) on pages 316-317, DOI: 10.11646/zootaxa.4058.3.1, http://zenodo.org/record/236980
Dose dependent effects of passive smoke on vascular function
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Secondhand smoke remains a significant public health threat. Despite recent public health initiatives attempting to limit this threat, more work is needed to understand fully its damaging effects. Data suggests that for every 8 people who die from smoking related illnesses 1 person dies from secondhand smoke exposure. While the dose of smoke inhaled from secondhand smoke is only a fraction of that inhaled from active smoking, the cardiovascular complications are nearly as large. Several large studies have suggested that local governmental restrictions on smoking in workplaces and public spaces have lowered the rates of heart attacks in the community. Despite these findings skepticism persists about how such low levels of smoking exposure can have such damaging health effects. Within the scientific community, little is known about how everyday low doses of smoke encountered in the community affects the cardiovascular system.

 This study hope to fill several gaps in our scientific knowledge and answer a simple question: what are the vascular effects of very low doses of smoke on healthy individuals who are exposed? Moreover, does being exposed to very low amounts of smoke have the same detrimental effects as higher amounts? We plan to study healthy volunteers and expose them to very low levels of secondhand smoke for a short duration in a custom built smoking chamber, mimicking exposures seen in public places. We will then measure how their blood vessels respond after being exposed to secondhand smoke using a simple ultrasound technique that has been shown to predict long term cardiovascular complications. We will also measure several blood tests in to better understand the mechanism by which passive smoke leads to adverse cardiovascular complications.

 By gaining insights into the amount of smoke needed to injure the vascular system, and the mechanisms by which these injuries occur, we hope to discover important information that can inform future public health policy discussions and allow physicians to better counsel their patients about secondhand smoke. We also hope to gain a better understanding of the science by which environmental exposures can injure the vascular system.
Additional file 2: Figure S2. of Combined platelet-rich plasma and lipofilling treatment provides great improvement in facial skin-induced lesion regeneration for scleroderma patients
Creators: name: VirzĂŹ, Francesco
name: Bianca, Paola
name: Giammona, Alessandro
name: Apuzzo, Tiziana
name: Franco, Simone Di
name: Mangiapane, Laura
name: Colorito, Maria
name: Catalano, Dario
name: Scavo, Emanuela
name: Nicotra, Annalisa
name: Benfante, Antonina
name: Pistone, Giuseppe
name: Caputo, Valentina
name: Dieli, Francesco
name: Pirrello, Roberto
name: Stassi, Giorgio
Publisher: name: Figshare
Year: 2017
Subjects: subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Physiology
subject: Ecology
subject: Immunology
subject: FOS: Clinical medicine
subject: Developmental Biology
subject: Hematology

description: showing AD-MSCs from SSc patients with decreased levels of mesenchymal stem cell markers. Representative dot plot showing the expression of CD44, CD90, CD271, CD29, CD73, and CD9 in AD-MSCs from healthy subjects (upper panels) and SSc patients (lower panels). Small boxes show isotype match control staining. (PDF 316Â kb)
Liolaemus melaniceps Pincheira-Donoso & Nunez 2005
Creators: name: Pincheira-Donoso, Daniel
name: Scolaro, J. Alejandro
name: Sura, Piotr
Publisher: name: Zenodo
Year: 2008
Subjects: Biodiversity
Taxonomy
Animalia
Chordata
Reptilia
Squamata
Liolaemidae
Liolaemus
Liolaemus melaniceps

description: Liolaemus melaniceps Pincheira-Donoso & Núñez
Liolaemus (Liolaemus) melaniceps Pincheira-Donoso & Núñez 2005: 398 (type locality: Chungungo Island [29°24’S, 71°21’W], Coquimbo Region, Chile).
description: Published as part of Pincheira-Donoso, Daniel, Scolaro, J. Alejandro & Sura, Piotr, 2008, A monographic catalogue on the systematics and phylogeny of the South American iguanian lizard family Liolaemidae (Squamata, Iguania), pp. 1-85 in Zootaxa 1800 on page 27, DOI: 10.5281/zenodo.6789337
Drosophila melanogaster Meigen
Creators: name: Sinclair, Bradley J.
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Drosophilidae
Drosophila
Drosophila melanogaster

description: melanogaster Meigen
Drosophila melanogaster Meigen, 1830: 85.
Drosophila (Sophophora) melanogaster: Carson et al. 1983: 241 [records]; Peck et al. 1998: 228 [introduced species]; Causton et al. 2006: 134 [introduced species].
Distribution. Introduced. Cosmopolitan; Galápagos: Santa Cruz.
Remarks. This species is found in the lowland zones (Carson et al. 1983).
description: Published as part of Sinclair, Bradley J., 2023, An annotated checklist of the Diptera of the Galápagos Archipelago (Ecuador), pp. 1-102 in Zootaxa 5283 (1) on page 56, DOI: 10.11646/zootaxa.5283.1.1, http://zenodo.org/record/7912667
Effect of tobacco toxins on human germ cell development
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Little is known about the effect of chemicals found in agents such as cigarette smoke on human germ cell (egg and sperm) development and the success of reproduction. A few studies have shown that cigarette smoking decreases the age at menopause in women. Yet, whether this is a direct or indirect effect on the germ cells themselves is not known. In this research plan, I will explore the use of human embryonic stem cells (hESCs) to study human germ cell development in the presence and absence of chemicals found in cigarette smoke. This system promises to provide an excellent opportunity to study environmental and genetic factors in human germ cell development, especially the early developmental stage from embryonic stem cells to primordial germ cells. In particular, over the last year, since I joined the Reijo Pera laboratory, I have been focused on the earliest events in germ cell development, whereas others have been focused on development of more mature oocytes. In particular, I have shown that germ cell development from human embryonic stem cells is responsive to growth factors that are known to be involved during normal fetal development in mice. My overall hypothesis that I wish to examine during my postdoctoral work is that human embryonic stem cells provide an excellent system to study the initial formation of germ cells and its susceptibility to genetic and environmental perturbation, including perturbation by toxins in smoke.

 My specific aims are:

 Aim 1: Enhance and characterize differentiation of hESCs to primordial germ cells in vitro.

 Aim 2: Examine the effect of PAHs during in vitro differentiation of hESCs to primordial germ cells.

 Aim 3: Silence AHR and BAX genes to probe the molecular pathway of PAHs effect on human germ cell development to primordial germ cells.

 I expect that with the completion of my aims, I will have enhanced the use of hESC differentiation to primordial germ cells to study the effect of tobacco smoke in normal and mutant human embryonic stem cell lines. I also expect that other researchers will follow up on these results and increasingly turn to the human embryonic stem cell system to probe effects of environmental toxins including others in tobacco smoke.
Additional file 1: of An Arabidopsis introgression zone studied at high spatio-temporal resolution: interglacial and multiple genetic contact exemplified using whole nuclear and plastid genomes
Creators: name: Hohmann, Nora
name: Koch, Marcus
Publisher: name: Figshare
Year: 2017
Subjects: subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Evolutionary Biology
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Result of SplitsTree network reconstruction. (PDF 770Â kb)
Drosophila nebulosa Sturtevant 1916
Creators: name: Sinclair, Bradley J.
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Drosophilidae
Drosophila
Drosophila nebulosa

description: nebulosa Sturtevant
Drosophila nebulosa Sturtevant, 1916: 327. Wheeler 1961: 750 [records]; Linsley & Usinger 1966: 169 [checklist]; Delgado Zúñiga 1975: 38 [chromosomal analysis].
Drosophila (Sophophora) nebulosa: Carson et al. 1983: 241 [records].
Distribution. Introduced. Neotropical; Galápagos: Isabela, San Cristóbal, Santa Cruz.
Remarks. Adults of this species were collected in the agricultural zone (Carson et al. 1983) and were reared from oranges (Wheeler 1961).
description: Published as part of Sinclair, Bradley J., 2023, An annotated checklist of the Diptera of the Galápagos Archipelago (Ecuador), pp. 1-102 in Zootaxa 5283 (1) on page 56, DOI: 10.11646/zootaxa.5283.1.1, http://zenodo.org/record/7912667
Rodolia lydiae Chazeau
Creators: name: Nattier, Romain
name: Jourdan, Hervé
name: Mille, Christian
name: Chazeau, Jean
Publisher: name: Zenodo
Year: 2015
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Coleoptera
Coccinellidae
Rodolia
Rodolia lydiae

description: Rodolia lydiae Chazeau
Rodolia lydiae Chazeau, 1991: 155.
This is an endemic species from New Caledonia, only recorded from dense rain forest and associated ultramafic shrubs in southern Grande Terre (Rivière Bleue, Forêt de la Thi). It has been recorded feeding on Aleyrodidae (Chazeau 1991).
Material examined. NEW CALEDONIA: Forêt de la Thi, 18.xi.1983 (J. Chazeau rec.) (ONNC); Forêt de la Thi, viii.1979 (J. Chazeau rec.) (ONNC); Rivière Bleue (parc.6), 160m, forêt dense sur alluvions, 3.xi.1986 (J. Chazeau rec.) (ONNC); Rivière Bleue (parc.6), 160m, forêt humide sur alluvions, 1328. x.1986 (L. Bonnet de Larbogne & J. Chazeau rec.) (ONNC); Rivière Bleue, maquis sur crête, 310m, i-vi.1987 (L. Bonnet de Larbogne, J. Chazeau, A. & S. Tillier rec.) (ONNC).
description: Published as part of Nattier, Romain, Jourdan, Hervé, Mille, Christian & Chazeau, Jean, 2015, An annotated checklist of the Coccinellidae (Coleoptera) from New Caledonia, pp. 301-331 in Zootaxa 4058 (3) on page 317, DOI: 10.11646/zootaxa.4058.3.1, http://zenodo.org/record/236980
Development of Fluorescent Probes for Sensing DNA Mismatches
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: DNA damage induces human cancers. UV light or genotoxic chemicals cause DNA injury (base mismatches) contributing to genetic instability. Exposure to carcinogens in tobacco smoke is mostly responsible for cancers in human. Permanent DNA sequence changes (mutations) are a major source of cancers. Mismatch repair (MMR) machinery in biology repairs these DNA mismatches. If they are left without repair, they may increase the possibility and danger of cancers. Human hereditary nonpolyposis colon cancer and sporadic cancers are two examples produced by this. A tool for specific single base mismatch detection in DNA is required to monitor cancers in early stages, which may prevent diseases and help develop suitable diagnostics and chemotherapeutics. Fluorescent sensors provide a non-invasive and convenient means to visualize analytes in biological systems. A probe that consists of a fluorophore coupled to a base mismatch recognizing metal complexes will allow imaging of DNA mismatches in vivo.
Additional file 1: of An Arabidopsis introgression zone studied at high spatio-temporal resolution: interglacial and multiple genetic contact exemplified using whole nuclear and plastid genomes
Creators: name: Hohmann, Nora
name: Koch, Marcus
Publisher: name: Figshare
Year: 2017
Subjects: subject: Medicine
subject: Genetics
subject: FOS: Biological sciences
subject: Evolutionary Biology
subject: Sociology
subject: FOS: Sociology
subject: 69999 Biological Sciences not elsewhere classified
subject: Infectious Diseases
subject: FOS: Health sciences

description: Result of SplitsTree network reconstruction. (PDF 770Â kb)
Drosophila simulans Sturtevant 1919
Creators: name: Sinclair, Bradley J.
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Drosophilidae
Drosophila
Drosophila simulans

description: simulans Sturtevant
Drosophila simulans Sturtevant, 1919: 153. Wheeler 1961: 750 [records]; Linsley & Usinger 1966: 169 [checklist]; Delgado Zúñiga 1975: 38 [chromosomal analysis].
Drosophila (Sophophora) simulans: Carson et al. 1983: 241 [records]; Peck et al. 1998: 228 [introduced species]; Causton et al. 2006: 134 [introduced species].
Distribution. Introduced. Cosmopolitan; Galápagos: Baltra, Isabela, San Cristóbal, Santa Cruz.
Remarks. Adults of this species were collected in the agricultural zone (Carson et al. 1983) and were reared from oranges (Wheeler 1961).
description: Published as part of Sinclair, Bradley J., 2023, An annotated checklist of the Diptera of the Galápagos Archipelago (Ecuador), pp. 1-102 in Zootaxa 5283 (1) on page 56, DOI: 10.11646/zootaxa.5283.1.1, http://zenodo.org/record/7912667
Plugstars une alternative aux trous noirs
Creators: name: Jean-Pierre Petit
Publisher: name: Zenodo
Year: 2024

description: On ne dispose, en tant que données directes, que du rapport des températures maximales et minimale, proche de 3, émanant des images de objets hypermassifs desgalaxies M 87 et Voie Lactée. S’agissant des trous noirs on recense ce qui a été à la base de ce modèle, la confusion de la grandeur intermédiaire R du premier article de Schwarzschild avec une coordonnée radiale r, susceptible de prendre des valeurs inférieures à la longueur caractéristique α , qu’on retrouve dans l’article publié en décembre 1916 par D .Hilbert. A cela s’ajoute, avec le changement de signature de la métrique, la perte de repère que représentait l’exigence d’une longueur s, donc d’un temps propre, réels. On montre, même en considérant la géométrie issue de la solution de l’équation d’Einstein à second membre nul, le caractère fallacieux d’une description d’un objet résolument non contractile, telle qu’à l’intérieur de la sphère de Schwarzschld r devienne la coordonnée de temps et t une coordonnée d’espace. On signale enfin la non prise en compte de la criticité physique introduite dans le second article de Schwarzschild, se produisant avant que la classique criticité géométrique ne puisse se manifester. En reprenant ces éléments, après avoir évoqué l’alternative des gravastars, on propose le modèle alternatif de plugstars où toutematière en excès est éliminée par inversion et éjection hors de l’objet. Après avoir justifié le fait que la rotation ait peu d’effet dans les objets hypermassifs et les avoir identifiés à des objets subcritiques on montre que le rapport des longueurs d’onde, maximale et minimale, dans ces condition proche de 3, s’accorde avec les données observationnelles issues des objets hypermassifs situés au centre des galaxies M 87 et Voie Lactée.
Rodolia pronuba Chazeau
Creators: name: Nattier, Romain
name: Jourdan, Hervé
name: Mille, Christian
name: Chazeau, Jean
Publisher: name: Zenodo
Year: 2015
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Coleoptera
Coccinellidae
Rodolia
Rodolia pronuba

description: Rodolia pronuba Chazeau
Rodolia pronuba Chazeau, 1991: 157.
Endemic to New Caledonia, this species is only known by the type specimen (from La Comboui Valley). It has been recorded feeding on ensign scale insects (Hemiptera: Ortheziidae).
Material examined. NEW CALEDONIA: Vallée de la Comboui, environs côte, maquis haut 210m, xi.1985 (J. Chazeau rec.) (ONNC).
description: Published as part of Nattier, Romain, Jourdan, Hervé, Mille, Christian & Chazeau, Jean, 2015, An annotated checklist of the Coccinellidae (Coleoptera) from New Caledonia, pp. 301-331 in Zootaxa 4058 (3) on page 317, DOI: 10.11646/zootaxa.4058.3.1, http://zenodo.org/record/236980
Liolaemus melanopleurus
Creators: name: Pincheira-Donoso, Daniel
name: Scolaro, J. Alejandro
name: Sura, Piotr
Publisher: name: Zenodo
Year: 2008
Subjects: Biodiversity
Taxonomy
Animalia
Chordata
Reptilia
Squamata
Liolaemidae
Liolaemus
Liolaemus melanopleurus

description: Liolaemus melanopleurus (Philippi)
Proctotretus melanopleurus Philippi 1860: 166 (type locality: “ Atacama Desert, Chile ”).
Liolaemus melanopleurus Etheridge 1995: 34; Etheridge & Espinoza 2000: 7; Sura 2005: 429.
Liolaemus (Liolaemus) melanopleurus Pincheira-Donoso & Núñez 2005: 338.
description: Published as part of Pincheira-Donoso, Daniel, Scolaro, J. Alejandro & Sura, Piotr, 2008, A monographic catalogue on the systematics and phylogeny of the South American iguanian lizard family Liolaemidae (Squamata, Iguania), pp. 1-85 in Zootaxa 1800 on page 27, DOI: 10.5281/zenodo.6789337
The molecular basis of apoE and LDL receptor interaction
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Mainstream and environmental tobacco smoke exposure represent significant risk factors for cardiovascular disease in men and women. The relationship between smoking and cardiovascular disease results from multiple mechanisms that interact to contribute to vascular injury and atherosclerosis lesion development. We are only beginning to understand how smoking contributes to the initiation and progression of cardiovascular disease. One area of intense interest is the effect of tobacco smoke on plasma lipoprotein particles. These particles, which normally function in transport of insoluble lipids through the bloodstream, have been recognized as targets for tobacco combustion-related oxidative damage. A specific type of lipoprotein, termed low density lipoprotein, is adversely affected by oxidation, being converted to a species that accelerates the progression of atherosclerosis lesion development. From knowledge that oxidized low density lipoproteins accumulate at the site of atherosclerosis lesion development, it can be concluded that susceptibility of these particles to oxidative modification caused by tobacco smoke exposure may constitute a contributing factor to disease progression.

 The goal of our research is to understand the molecular basis whereby a protein component of circulating lipoproteins facilitates their removal from the circulation and hence, decreases the amount of low density lipoprotein available for oxidative modification. Apolipoprotein E associates with the surface of plasma lipoprotein particles and mediates interaction with cell surface receptor molecules. We will conduct studies designed to ascertain the interaction between apolipoprotein E and low density lipoprotein receptor. We will evaluate the role of a specific architectural feature both of apolipoprotein E and low density lipoprotein receptor as a modulator of cholesterol homeostasis. These studies will increase our knowledge about the molecular mechanism between apolipoprotein E and low density lipoprotein receptor to regulate lipoprotein levels in plasma and provide the basis for new strategies to decrease tobacco smoke exposure related lipoprotein damage that promotes and accelerates the pathology of cardiovascular disease. Results obtained from my proposed research will be useful in the design of therapeutic strategies to prevent tobacco related disease.
Scymnini Mulsant 1846
Creators: name: Nattier, Romain
name: Jourdan, Hervé
name: Mille, Christian
name: Chazeau, Jean
Publisher: name: Zenodo
Year: 2015
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Coleoptera
Coccinellidae

description: SCYMNINI Mulsant, 1846
The biology of most New Caledonian ladybugs recorded from this tribe is unknown. They are all considered to be aphid specialists or scale insect feeders, and have potential as important biocontrol agents.
description: Published as part of Nattier, Romain, Jourdan, Hervé, Mille, Christian & Chazeau, Jean, 2015, An annotated checklist of the Coccinellidae (Coleoptera) from New Caledonia, pp. 301-331 in Zootaxa 4058 (3) on page 317, DOI: 10.11646/zootaxa.4058.3.1, http://zenodo.org/record/236980
Drosophila willistoni Sturtevant 1916
Creators: name: Sinclair, Bradley J.
Publisher: name: Zenodo
Year: 2023
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Diptera
Drosophilidae
Drosophila
Drosophila willistoni

description: willistoni Sturtevant
Drosophila willistoni Sturtevant, 1916: 327. Johnson 1924: 90 [records]; Curran 1932: 355 [records]; Linsley & Usinger 1966: 169 [checklist]; Delgado Zúñiga 1975: 38 [chromosomal analysis].
Drosophila (Sophophora) willistoni: Brake & Bächli 2008: 119 [catalogue].
Drosophila (Sophophora) willistoni quechua Ayala, 1973: 276. Carson et al. 1983: 241 [records]; Brake & Bächli 2008: 119 [as syn. of D. willistoni].
Distribution. Introduced. Nearctic, Neotropical; Galápagos: Baltra, Isabela, San Cristóbal, Santa Cruz.
Remarks. Adults of this species were found in highland zones, primarily in the agricultural zone (Carson et al. 1983).
description: Published as part of Sinclair, Bradley J., 2023, An annotated checklist of the Diptera of the Galápagos Archipelago (Ecuador), pp. 1-102 in Zootaxa 5283 (1) on page 57, DOI: 10.11646/zootaxa.5283.1.1, http://zenodo.org/record/7912667
Additional file 1: of Exercise and cancer-related fatigue in adults: a systematic review of previous systematic reviews with meta-analyses
Creators: name: Kelley, George
name: Kelley, Kristi
Publisher: name: Figshare
Year: 2017
Subjects: subject: Space Science
subject: Medicine
subject: 80699 Information Systems not elsewhere classified
subject: FOS: Computer and information sciences
subject: Developmental Biology
subject: Science Policy

description: Search strategies used for each database. This file includes the search strategies used for all of our electronic databases searches. These include PubMed, Sport Discus, Web of Science, Scopus, Cochrane, ProQuest Dissertations and Theses. (DOCX 224 kb)
nAChR mediated dopamine release after chronic nicotine
Creators: name: University Of California Office Of The President
Publisher: name: University of California Office of the President
publisherIdentifier: https://ror.org/00dmfq477
publisherIdentifierScheme: ROR
Year: 2024

description: Parkinson’s disease is a debilitating disorder that affects over four million people over the age of 60 worldwide. The most common symptoms are resting tremor, muscular rigidity, bradykinesia (slowed movement) and postural instability. The cause of this neurodegenerative disorder is currently unknown. Strong evidence shows that smokers are less likely to develop Parkinson’s disease. These findings have sparked wide interest on finding the component of cigarette smoke responsible for the neuroprotective effect of smoking. Thus far, nicotine has become the main candidate reported to be responsible for protecting neurons from degeneration. Nicotine is known to stimulate the function of brain regions that are affected by Parkinson’s disease and some studies have shown that nicotine can protect nerve cells from being damaged. The long-term goal of the present proposal is to understand how nicotine may be executing its neuroprotection in Parkinson’s disease. Our preliminary data demonstrate that nicotine activates receptors in neurons responsible for motor control. The activation of these receptors leads to the release of chemicals in the brain that modulate the actions of these neurons. In this proposal we will investigate further how the activation of these receptors changes the kinetics of release in these neurons that control motor function. This will be done in control animals and also in animals with brain damage similar to that in Parkinson’s disease that have being treated chronically with nicotine. The results obtained in our studies will give us a better understanding of how nicotine changes the dynamics of receptor activation and release under physiological conditions and with disease. This information can then be used to develop therapeutics to prevent or stop the degeneration of neurons that leads to the symptoms of Parkinson’s disease.
Liolaemus monticola Muller & Hellmich 1932
Creators: name: Pincheira-Donoso, Daniel
name: Scolaro, J. Alejandro
name: Sura, Piotr
Publisher: name: Zenodo
Year: 2008
Subjects: Biodiversity
Taxonomy
Animalia
Chordata
Reptilia
Squamata
Liolaemidae
Liolaemus
Liolaemus monticola

description: Liolaemus monticola Müller & Hellmich
Liolaemus monticola monticola Müller & Hellmich 1932c: 177 (type locality: Río San Francisco Valley, Santiago, Metropolitan Region, Chile); Burt & Burt 1933: 34; Hellmich 1934: 87; Donoso-Barros & Candiani 1950: 488; DonosoBarros 1966a: 226, 1970a: 186, 1970b: 94; Fuentes & Ipinza 1979: 123; Vanzolini 1986: 15; Veloso & Navarro 1988: 507; Laurent 1992: 32; Núñez & Jaksic 1992: 74; Etheridge 1995: 33; Etheridge & Espinoza 2000: 7.
Liolaemus (Liolaemus) monticola Pincheira-Donoso & Núñez 2005: 372.
Liolaemus monticola Pflaumer 1944: 113; Porter 1972: 401; Mattison 1999: 60; Escobar et al. 2001: 1679; Fox & Shipman 2003: 321; Sura 2005: 429; Díaz & Lobo 2006: 264; Labra 2006: 993.
Observations: Over the last two decades, a series of karyotypic studies conducted in different apparently isolated populations assigned to L. monticola have suggested that they might have experienced processes of genetic diversification (Lamborot et al. 1979, 1981, 2003; Lamborot 1985 a, b, 1991, 1993, 1998, 2001; Lamborot & Eaton 1992, 1997; Lamborot & Alvarez-Sarret 1993). Nevertheless, although karyological aspects have been explored thoroughly, additional detailed studies are needed to evaluate the impact of geographical barriers, such as rivers, in driving incipient differentiation in these lizard populations (Núñez & PincheiraDonoso 2006; see Lamborot & Eaton 1992, 1997; Lamborot et al. 2003 for morphological information). Since it is not entirely clear whether all these studied populations are indeed L. monticola (Pincheira-Donoso & Núñez 2005), we do not refer most of these studies in the nomenclatural history of this taxon.
description: Published as part of Pincheira-Donoso, Daniel, Scolaro, J. Alejandro & Sura, Piotr, 2008, A monographic catalogue on the systematics and phylogeny of the South American iguanian lizard family Liolaemidae (Squamata, Iguania), pp. 1-85 in Zootaxa 1800 on page 27, DOI: 10.5281/zenodo.6789337
Apolinus lividigaster Mulsant
Creators: name: Nattier, Romain
name: Jourdan, Hervé
name: Mille, Christian
name: Chazeau, Jean
Publisher: name: Zenodo
Year: 2015
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Coleoptera
Coccinellidae
Apolinus
Apolinus lividigaster

description: Apolinus lividigaster (Mulsant)
Platyomus lividigaster Mulsant, 1853: 286.
Scymnodes (Apolinus) lividigaster: Pope & Lawrence 1990: 244. Apolinus lividigaster: Slipinski 2007: 99.
This species was originally described from Australia; it is also recorded from New Zealand and Hawaii. In New Caledonia, it was not recorded by Bielawski (1972) in his review of New Caledonian Scymnini. It has only been collected in Nouméa and Yaté regions (southern Grande Terre). It is predominantly aphidiphagous, but is also able to use pollen when preys are scarce (Lundgren 2009). Based on this information, we consider it to be a recent arrival because of the lack of autochthonous aphids, whether by natural spread or accidental introduction.
Material examined. NEW CALEDONIA: Nouméa, Anse Vata, ix.1976 (A. Delobel rec.) (ONNC); Nouméa, Ouen Toro, 24.vi.1978 (A. Delobel rec.) (ONNC); St Gabriel (Yaté), 0-200m, xi.1986 (N.L.H. Krauss rec.)
(ONNC); Farino, "Tendéa", 21.61928°S / 165.70907°E / 403m, 29.iv.2004 (S. Cazères rec.) (CXMNC); Ouvéa, Hynthot, 30.vi.2008 (C. Mille rec.) (CXMNC); Thio, tribu de Koua, COL /ins/ 28-14, 18.iii.2013 (R-M. M'Boueri rec.) (CXMNC); Ouvéa, COL /ins/87-14, 14.iii.2014 (C. Mille rec.) (CXMNC); Bourail, Gouaro, 21.58113°S / 165.42045°E / 68m, ii.2001 (S. Cazères rec.) (CXMNC); Thio, Dothio, 21°35.305'S / 165°09.084'E / 18m, 04.v.2006 (S. Cazères rec.) (CXMNC); Nouméa, 15.xii.1981 (R. Catala rec.) (MNHN).
description: Published as part of Nattier, Romain, Jourdan, Hervé, Mille, Christian & Chazeau, Jean, 2015, An annotated checklist of the Coccinellidae (Coleoptera) from New Caledonia, pp. 301-331 in Zootaxa 4058 (3) on pages 317-318, DOI: 10.11646/zootaxa.4058.3.1, http://zenodo.org/record/236980