title: ÐÑÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð¸ Ñ Ð°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ° Î±-L-ÑÑÐºÐ¾Ð·Ð¸Ð´Ð°Ð·Ñ Ð¸Ð· Paralithodes camtschaticus
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Purification and characterization of Î±-L-fucosidase from Paralithodes camtschaticus
Creators: name: ÐÑÐ¸Ð¼Ð¾Ð², ÐÑÑÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÐÐ¸Ð¾ÑÐµÑ Ð½Ð¾Ð»Ð¾Ð³Ð¸Ñ
ÑÑÐºÐ¾Ð¸Ð´Ð°Ð½
ÑÑÐ°Ð½ÑÑÑÐºÐ¾Ð·Ð¸Ð»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
fucoidan
transfucosylation

description: Ð¢ÐµÐ¼Ð° Ð²ÑÐ¿ÑÑÐºÐ½Ð¾Ð¹ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ: Â«ÐÑÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ Ð¸ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐ° Î±-L-ÑÑÐºÐ¾Ð·Ð¸Ð´Ð°Ð·Ñ Ð¸Ð· Paralithodes camtschaticusÂ». ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐµÑÐ¼ÐµÐ½ÑÐ° Î±-L-ÑÑÐºÐ¾Ð·Ð¸Ð´Ð°Ð·Ñ Ð¸Ð· ÐºÑÐ°Ð±Ð° ÐºÐ°Ð¼ÑÐ°ÑÑÐºÐ¾Ð³Ð¾. ÐÐ¾ÑÐ»Ðµ Ð¿ÐµÑÐ²Ð¾Ð½Ð°ÑÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÐºÑÐ¸Ð½Ð¸Ð½Ð³Ð° Ð³Ð»Ð¸ÐºÐ¾Ð·Ð¸Ð´-Ð³Ð¸Ð´ÑÐ¾Ð»Ð°Ð·Ð½ÑÑ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐµÐ¹ Ð¶Ð¸Ð´ÐºÐ¾ÑÑÐ¸, ÑÐµÐºÑÐµÑÐ¸ÑÑÐµÐ¼Ð¾Ð¹ Ð³ÐµÐ¿Ð°ÑÐ¾Ð¿Ð°Ð½ÐºÑÐµÐ°ÑÐ¾Ð¼ P. camtschaticus, Ð¸ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¸Ñ Ð·Ð°Ð¼ÐµÑÐ½Ð¾Ð¹ Î±-L-ÑÑÐºÐ¾Ð·Ð¸Ð´Ð°Ð·Ð½Ð¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸, Ð±ÑÐ»Ð° ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð° ÑÑÐµÐ¼Ð° Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ñ Ð¸ Ð¾ÑÐ¸ÑÑÐºÐ¸ Ð´Ð°Ð½Ð½Ð¾Ð³Ð¾ ÑÐµÑÐ¼ÐµÐ½ÑÐ°, Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð° ÐµÐ³Ð¾ Ð¼Ð¾Ð»ÐµÐºÑÐ»ÑÑÐ½Ð°Ñ Ð¼Ð°ÑÑÐ° Ð² Ð½Ð°ÑÐ¸Ð²Ð½Ð¾Ð¼ ÑÐ¾ÑÑÐ¾ÑÐ½Ð¸Ð¸. ÐÐ»Ñ ÑÐµÐ°ÐºÑÐ¸Ð¸ Ð³Ð¸Ð´ÑÐ¾Ð»Ð¸Ð·Ð° Ð¼Ð¾Ð´ÐµÐ»ÑÐ½Ð¾Ð³Ð¾ ÑÑÐ±ÑÑÑÐ°ÑÐ° Ð¿Ð°ÑÐ°- Ð½Ð¸ÑÑÐ¾ÑÐµÐ½Ð¸Ð»-Î±-L-ÑÑÐºÐ¾Ð¿Ð¸ÑÐ°Ð½Ð¾Ð·Ð¸Ð´Ð°, ÐºÐ°ÑÐ°Ð»Ð¸Ð·Ð¸ÑÑÐµÐ¼Ð¾Ð¹ ÑÐµÑÐ¼ÐµÐ½ÑÐ¾Ð¼, Ð±ÑÐ»Ð¸ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ñ Ð¾Ð¿ÑÐ¸Ð¼Ð°Ð»ÑÐ½ÑÐµ Ð·Ð½Ð°ÑÐµÐ½Ð¸Ñ pH Ð¸ ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÑ, ÑÐ°ÐºÐ¶Ðµ Ð±ÑÐ»Ð¾ Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð´Ð°Ð½Ð½ÑÑ Ð¿Ð¾ÐºÐ°Ð·Ð°ÑÐµÐ»ÐµÐ¹ Ð½Ð° ÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾ÑÑÑ ÑÐµÑÐ¼ÐµÐ½ÑÐ°. ÐÐ»Ñ ÑÑÐ¾Ð¹ Ð¶Ðµ ÑÐµÐ°ÐºÑÐ¸Ð¸ Ð±ÑÐ»Ð¸ ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ñ ÐºÐ¸Ð½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ðµ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÑ: ÐºÐ¾Ð½ÑÑÐ°Ð½ÑÐ° ÐÐ¸ÑÐ°ÑÐ»Ð¸ÑÐ° Ð¸ ÐºÐ¾Ð½ÑÑÐ°Ð½ÑÐ° Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¿ÑÐ¾Ð´ÑÐºÑÐ¾Ð¼ ÑÐµÐ°ÐºÑÐ¸Ð¸ L-ÑÑÐºÐ¾Ð·Ð¾Ð¹. ÐÑÐ»Ð° Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð° ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ Î±-L- ÑÑÐºÐ¾Ð·Ð¸Ð´Ð°Ð·Ñ ÐºÐ°ÑÐ°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°ÑÑ ÑÐµÐ°ÐºÑÐ¸Ñ ÑÑÐ°Ð½ÑÑÑÐºÐ¾Ð·Ð¸Ð»Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ, Ð¸ÑÐ¿Ð¾Ð»ÑÐ·ÑÑ Ð¼Ð¾Ð´ÐµÐ»ÑÐ½ÑÐ¹ ÑÑÐ±ÑÑÑÐ°Ñ Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð´Ð¾Ð½Ð¾ÑÐ° Ð¸ Ð°ÐºÑÐµÐ¿ÑÐ¾ÑÐ°, Ð° ÑÐ°ÐºÐ¶Ðµ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ ÑÐ°ÑÑÐµÐ¿Ð»ÑÑÑ ÑÐ°ÑÑÐ¸ÑÐ½Ð¾-Ð³Ð¸Ð´ÑÐ¾Ð»Ð¸Ð·Ð¾Ð²Ð°Ð½Ð½ÑÐ¹ ÑÑÐºÐ¾Ð¸Ð´Ð°Ð½ Ñ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸ÐµÐ¼ L-ÑÑÐºÐ¾Ð·Ñ. Ð ÐµÐ·ÑÐ»ÑÑÐ°ÑÑ ÑÐ°Ð±Ð¾ÑÑ Ð¿Ð¾ÑÐ»ÑÐ¶Ð°Ñ Ð¾ÑÐ½Ð¾Ð²Ð¾Ð¹ Ð´Ð»Ñ Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐ¸Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¹ ÑÐµÑÐ¼ÐµÐ½ÑÐ° Î±-L-ÑÑÐºÐ¾Ð·Ð¸Ð´Ð°Ð·Ñ Ñ ÑÐµÐ»ÑÑ ÐµÐ³Ð¾ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ Ð¿ÑÐ°ÐºÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð² Ð±Ð¸Ð¾ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¸ Ð¼ÐµÐ´Ð¸ÑÐ¸Ð½Ðµ.
description: The subject of the graduate qualification work is âPurification and characterization of Î±-L-fucosidase from Paralithodes camtschaticusâ. This work is devoted to the study of Î±-L-fucosidase from red king crab. After initial screening for glycoside hydrolase activities in the fluid secreted by the P. camtschaticus hepatopancreas and the discovery of notable Î±-L-fucosidase activity, a scheme of isolation and purification of this enzyme was developed. Enzymeâs molecular weight under nondenaturing conditions was determined. For the hydrolysis reaction of the model substrate p-nitrophenyl-Î±-L-fucopyranoside catalyzed by the enzyme, we determined the optimal pH and temperature values as well as the effect of these parameters on the stability of the enzyme. For the same reaction, the kinetic parameters were established: the Michaelis constant and the constant of inhibition by the reaction product L-fucose. It is shown that Î±-L-fucosidase is able to catalyze the transglycosylation reaction using a model substrate as a donor and acceptor. The enzymeâs ability to cleave partially hydrolyzed fucoidan to produce L-fucose was established. The results of the work will serve as a basis for further studies of Î±-L- fucosidase with the aim of its effective practical application in biotechnology and medicine.