title: ÐÐ½ÐµÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐµ Ð²ÐµÐ·Ð¸ÐºÑÐ»Ñ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹ ÐºÐ°Ðº Ð¿ÐµÑÐµÐ½Ð¾ÑÑÐ¸ÐºÐ¸ ÑÐ½Ð´Ð¾Ð³ÐµÐ½Ð½ÑÑ Ð¸ ÑÐºÐ·Ð¾Ð³ÐµÐ½Ð½ÑÑ Ð±Ð¸Ð¾Ð¼Ð¾Ð»ÐµÐºÑÐ»
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Microalgae extracellular vesicles as delivery of endogenous and exogenous biomolecules
Creators: name: ÐÑÑÐµÐ²Ð¸Ñ, ÐÐ»ÐµÐ½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð²Ð½ÐµÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐµ Ð²ÐµÐ·Ð¸ÐºÑÐ»Ñ
Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ð¸ Parachlorella kessleri
Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»Ð¸ Chlamydomonas reinhardi
Ð¿ÐµÑÐµÐ½Ð¾ÑÑÐ¸ÐºÐ¸ Ð»ÐµÐºÐ°ÑÑÑÐ²ÐµÐ½Ð½ÑÑ Ð²ÐµÑÐµÑÑÐ²
ÑÐ½Ð´Ð¾Ð³ÐµÐ½Ð½ÑÐµ Ð±Ð¸Ð¾Ð¼Ð¾Ð»ÐµÐºÑÐ»Ñ
extracellular vesicles
microalgae Parachlorella kessleri
microalgae Chlamydomonas reinhardi
drug delivery system
endogenous biomolecules

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð²Ð½ÐµÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð²ÐµÐ·Ð¸ÐºÑÐ» (ÐÐ), Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½ÑÑ Ð¸Ð· ÐºÑÐ»ÑÑÑÑÐ°Ð»ÑÐ½Ð¾Ð¹ ÑÑÐµÐ´Ñ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹ Parachlorella kessleri (ÐÐ-Ð¥Ð») Ð¸ Chlamydomonas reinhardi (ÐÐ-Ð¥Ð»Ð). Ð ÑÐ¾Ð´Ðµ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð° Ð¿ÑÐ¾Ð¸Ð·Ð²ÐµÐ´ÐµÐ½Ð° Ð¾Ð¿ÑÐ¸Ð¼Ð¸Ð·Ð°ÑÐ¸Ñ ÑÑÐ»Ð¾Ð²Ð¸Ð¹ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹ Ð´Ð»Ñ ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ñ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð° Ð²ÑÐ´ÐµÐ»ÑÐµÐ¼ÑÑ ÐÐ. ÐÑÐ»Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÐ½ÑÑ Ð¸ ÑÐ²ÐµÑÐ¾Ð²ÑÑ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÐ¾Ð² ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ, Ð° ÑÐ°ÐºÐ¶Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ ÑÐµÐ¿Ð»Ð¾Ð²Ð¾Ð³Ð¾ ÑÐ¾ÐºÐ° Ð½Ð° ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ð¾ ÑÐµÐºÑÐµÑÐ¸ÑÑÐµÐ¼ÑÑ ÐÐ. Ð¡ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÑÐ°ÐºÐ¸Ñ Ð¼ÐµÑÐ¾Ð´Ð¾Ð², ÐºÐ°Ðº Ð°Ð½Ð°Ð»Ð¸Ð· ÑÑÐ°ÐµÐºÑÐ¾ÑÐ¸Ð¸ Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸Ñ (NTA), Ð°ÑÐ¾Ð¼Ð½Ð¾-ÑÐ¸Ð»Ð¾Ð²Ð°Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ (ÐÐ¡Ð) Ð¸ ÐºÑÐ¸Ð¾ÑÐ»ÐµÐºÑÑÐ¾Ð½Ð½Ð°Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ Ð±ÑÐ»Ð¸ Ð¾ÑÐµÐ½ÐµÐ½Ñ ÑÐ°Ð·Ð¼ÐµÑ Ð¸ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ñ ÐÐ. Ð¢Ð°ÐºÐ¶Ðµ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¾ÑÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐ²ÐµÑÐ¾ÑÐ°ÑÑÐµÑÐ½Ð¸Ñ Ð±ÑÐ» Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½ Ð´Ð·ÐµÑÐ°-Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð» ÐÐ. Ð ÑÐ¾Ð´Ðµ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ» Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½ Ð°Ð½Ð°Ð»Ð¸Ð· Ð¿ÑÐ¾ÑÐµÐ¾Ð¼Ð° ÐÐ. ÐÐ´ÐµÐ½ÑÐ¸ÑÐ¸ÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐµ Ð±ÐµÐ»ÐºÐ¸ Ð² ÑÐ¾ÑÑÐ°Ð²Ðµ Ð²ÐµÐ·Ð¸ÐºÑÐ» ÑÐºÐ°Ð·ÑÐ²Ð°ÑÑ Ð½Ð° ÑÑÐ°ÑÑÐ¸Ðµ ÐÐ Ð² Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸ÑÐµÑÐºÐ¸Ñ Ð¿ÑÐ¾ÑÐµÑÑÐ°Ñ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹. ÐÑÐ¾Ð¼Ðµ ÑÐ¾Ð³Ð¾, Ð±ÑÐ»Ð¾ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¾ Ð½ÐµÑÐºÐ¾Ð»ÑÐºÐ¾ Ð±ÐµÐ»ÐºÐ¾Ð² Ð¾Ð±Ð»Ð°Ð´Ð°ÑÑÐ¸Ñ Ð°Ð½ÑÐ¸Ð¾ÐºÑÐ¸Ð´Ð°ÑÐ¸Ð²Ð½ÑÐ¼Ð¸ ÑÐ²Ð¾Ð¹ÑÑÐ²Ð°Ð¼Ð¸. Ð ÑÐ°Ð±Ð¾ÑÐµ Ð±ÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ ÐÐ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹ Ð½Ðµ Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÑÑ ÑÐ¸ÑÐ¾ÑÐ¾ÐºÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÑÑÐµÐºÑÐ° Ð½Ð° ÐºÐ»ÐµÑÐºÐ¸ Ð½Ð¾ÑÐ¼Ð°Ð»ÑÐ½Ð¾Ð¹ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸, Ð½Ð¾ Ð¼Ð¾Ð³ÑÑ Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾Ð²Ð°ÑÑ Ð¿ÑÐ¾Ð»Ð¸ÑÐµÑÐ°ÑÐ¸Ñ Ð¾Ð¿ÑÑÐ¾Ð»ÐµÐ²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº. Ð¢Ð°ÐºÐ¶Ðµ Ð±ÑÐ»Ð° Ð¿ÑÐ¾Ð´ÐµÐ¼Ð¾Ð½ÑÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð° Ð²Ð¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾ÑÑÑ Ð²Ð²ÐµÐ´ÐµÐ½Ð¸Ñ Ð² ÑÐ¾ÑÑÐ°Ð² ÐÐ ÑÐµÐºÐ¾Ð¼Ð±Ð¸Ð½Ð°Ð½ÑÐ½Ð¾Ð³Ð¾ ÑÐµÐ»Ð¾Ð²ÐµÑÐµÑÐºÐ¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° HSP70, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð° ÑÑÐ°Ð²Ð½Ð¸ÑÐµÐ»ÑÐ½Ð°Ñ Ð¾ÑÐµÐ½ÐºÐ° ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð½Ð°Ð³ÑÑÐ·ÐºÐ¸ Ð¸ Ð·Ð°ÑÐ²Ð°ÑÐ° Ð½Ð°Ð³ÑÑÐ¶ÐµÐ½Ð½ÑÑ Ð²ÐµÐ·Ð¸ÐºÑÐ» ÐºÐ»ÐµÑÐºÐ°Ð¼Ð¸ ÑÐµÐ»Ð¾Ð²ÐµÐºÐ°. Ð¢Ð°ÐºÐ¸Ð¼ Ð¾Ð±ÑÐ°Ð·Ð¾Ð¼, Ð² ÑÐ°Ð±Ð¾ÑÐµ Ð¿ÑÐ¾Ð´ÐµÐ¼Ð¾Ð½ÑÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð° Ð¿ÐµÑÑÐ¿ÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÐÐ Ð¼Ð¸ÐºÑÐ¾Ð²Ð¾Ð´Ð¾ÑÐ¾ÑÐ»ÐµÐ¹ Ð² ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð´Ð¾ÑÑÐ°Ð²ÑÐ¸ÐºÐ¾Ð² ÑÐ½Ð´Ð¾Ð³ÐµÐ½Ð½ÑÑ Ð¸ ÑÐºÐ·Ð¾Ð³ÐµÐ½Ð½ÑÑ ÑÐµÑÐ°Ð¿ÐµÐ²ÑÐ¸ÑÐµÑÐºÐ¸Ñ Ð±Ð¸Ð¾Ð¼Ð¾Ð»ÐµÐºÑÐ».
description: The given work is devoted to the study of extracellular vesicles (EVs) isolated from the culture medium of microalgae Parachlorella kessleri (EV-Chl) and Chlamydomonas reinhardi (EV-ChlM). In this study, we analysed the change in the amount of EVs when the microalgae cultivation conditions varied. The size and morphology of EVs were studied by nanoparticle tracking analysis (NTA), atomic force microscopy (AFM), and cryo-electron microscopy. The zeta-potential of the EVs was also studied by electrophoretic light scattering. During the work, the protein profile of EVs was determined, which demonstrated the participation of EVs in the metabolic processes of microalgae, and several proteins with antioxidant activities were discovered. The work showed that microalgae EVs do not have a cytotoxic effect on cells of normal morphology but can inhibit the cell proliferation of tumor cells. The effectiveness of loading EVs with exogenous recombinant human HSP70 protein was also demonstrated that microalgal EVs are successfully captured by mammalian cells and deliver their contents. Thus, the work demonstrates the promise of microalgae EVs as delivery system of endogenous and exogenous therapeutic biomolecules.