title: ÐÐ¸Ð¿ÐµÑÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð±ÐµÐ»ÐºÐ° ÐÐ3 Ð¿ÑÐµÐ´Ð¾ÑÐ²ÑÐ°ÑÐ°ÐµÑ Ð´ÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸Ð²Ð½ÑÐµ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð² ÑÐ¸Ð½Ð°Ð¿ÑÐ°Ñ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð°Ð»ÑÐ½ÑÑ Ð½ÐµÐ¹ÑÐ¾Ð½Ð¾Ð² Ð² ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ ÐÐ»ÑÑÐ³ÐµÐ¹Ð¼ÐµÑÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Overexpression of the EB3 protein prevents degenerative changes in the synapses of hippocampal neurons in the cell model of Alzheimer's disease
Creators: name: Ð Ð°ÐºÐ¾Ð²ÑÐºÐ°Ñ, ÐÐ½Ð°ÑÑÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: Ð±Ð¾Ð»ÐµÐ·Ð½Ñ ÐÐ»ÑÑÐ³ÐµÐ¹Ð¼ÐµÑÐ°
Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð½Ð°Ñ ÑÐ¾ÐºÑÐ¸ÑÐ½Ð¾ÑÑÑ
Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿
Ð´Ð¸Ð½Ð°Ð¼Ð¸ÑÐµÑÐºÐ¸Ðµ Ð¼Ð¸ÐºÑÐ¾ÑÑÑÐ±Ð¾ÑÐºÐ¸
Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÐµ ÑÐ¸Ð¿Ð¸ÐºÐ¸
EB3
PSD-95
Alzheimerâs disease
amyloid toxicity
hippocampus
dynamic microtubules
dendritic spines

description: ÐÐµÐ»Ð¾Ðº EB3 ÐºÑÐµÐ¿Ð¸ÑÑÑ Ðº Ð¿Ð¾Ð»Ð¾Ð¶Ð¸ÑÐµÐ»ÑÐ½Ð¾Ð¼Ñ ÐºÐ¾Ð½ÑÑ ÑÐ°ÑÑÑÑÐ¸Ñ Ð½ÐµÐ¹ÑÐ¾Ð½Ð°Ð»ÑÐ½ÑÑ Ð¼Ð¸ÐºÑÐ¾ÑÑÑÐ±Ð¾ÑÐµÐº Ð¸ ÑÐµÐ³ÑÐ»Ð¸ÑÑÐµÑ Ð¸Ñ Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÑ. ÐÐ¸Ð¿ÐµÑÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ ÐÐ3 Ð²ÑÐ·ÑÐ²Ð°ÐµÑ Ð·Ð½Ð°ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ðµ ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ðµ ÑÐ¸ÑÐ»Ð° Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÑ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð² Ð³ÑÐ¸Ð±Ð¾Ð²Ð¸Ð´Ð½Ð¾Ð³Ð¾ ÑÐ¸Ð¿Ð° Ð² Ð¿ÐµÑÐ²Ð¸ÑÐ½ÑÑ Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð°Ð»ÑÐ½ÑÑ Ð½ÐµÐ¹ÑÐ¾Ð½Ð°Ñ. Ð ÑÐ°Ð±Ð¾ÑÐµ ÑÐ°ÑÑÐ¼Ð¾ÑÑÐµÐ½Ð¾ Ð½ÐµÐ¹ÑÐ¾Ð¿ÑÐ¾ÑÐµÐºÑÐ¾ÑÐ½Ð¾Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð±ÐµÐ»ÐºÐ° EB3 Ð½Ð° Ð³Ð¸Ð¿Ð¿Ð¾ÐºÐ°Ð¼Ð¿Ð°Ð»ÑÐ½ÑÐµ Ð½ÐµÐ¹ÑÐ¾Ð½Ñ Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ Ð½Ð¸Ð·ÐºÐ¾Ð¹ Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð½Ð¾Ð¹ ÑÐ¾ÐºÑÐ¸ÑÐ½Ð¾ÑÑÐ¸, Ð¼Ð¾Ð´ÐµÐ»Ð¸ÑÑÑÑÐµÐ¹ Ð±Ð¾Ð»ÐµÐ·Ð½Ñ ÐÐ»ÑÑÐ³ÐµÐ¹Ð¼ÐµÑÐ°. ÐÐ»Ð°ÑÑÐµÑÐ¸Ð·Ð°ÑÐ¸Ñ Ð±ÐµÐ»ÐºÐ° PSD-95 ÑÐ²ÑÐ·Ð°Ð½Ð° ÑÐ¾ ÑÑÐ°Ð±Ð¸Ð»ÑÐ½Ð¾ÑÑÑÑ Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÑ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð². ÐÐ²ÑÐ¾ÑÐ¾Ð¼ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½ Ð¼ÐµÑÐ¾Ð´Ð¸ÑÐµÑÐºÐ¸Ð¹ Ð¿Ð¾Ð´ÑÐ¾Ð´ Ð´Ð»Ñ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÐ¸ Ð¸ ÑÐ°Ð·Ð¼ÐµÑÐ¾Ð² ÐºÐ»Ð°ÑÑÐµÑÐ¾Ð² Ð±ÐµÐ»ÐºÐ° PSD-95 Ð² Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ°Ñ Ð½ÐµÐ¹ÑÐ¾Ð½Ð¾Ð² Ñ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸ÐµÐ¼ Ð»Ð¾ÐºÐ°Ð»ÑÐ½Ð¾Ð¹ Ð±Ð¸Ð½Ð°ÑÐ¸Ð·Ð°ÑÐ¸Ð¸ Ð¿Ð¾ÑÐ»Ðµ ÐºÐ¾ÑÑÐµÐºÑÐ¸Ð¸ ÑÐ¾Ð½Ð° Ð¸ ÑÑÑÑÐ°Ð½ÐµÐ½Ð¸Ð¸ ÑÑÐ¼Ð° Ð½Ð° ÐºÐ¾Ð½ÑÐ¾ÐºÐ°Ð»ÑÐ½ÑÑ Ð¸Ð·Ð¾Ð±ÑÐ°Ð¶ÐµÐ½Ð¸ÑÑ. ÐÐ¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð³Ð¸Ð¿ÐµÑÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð±ÐµÐ»ÐºÐ° EB3 Ð²ÑÐ·ÑÐ²Ð°ÐµÑ ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ðµ ÑÐ¸ÑÐ¸Ð½Ñ Ð³Ð¾Ð»Ð¾Ð²ÐºÐ¸ Ð³ÑÐ¸Ð±Ð¾Ð²Ð¸Ð´Ð½ÑÑ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð² Ð½ÐµÐ¹ÑÐ¾Ð½Ð¾Ð² Ð¸ Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÐ¸ ÐºÐ»Ð°ÑÑÐµÑÐ¾Ð² Ð±ÐµÐ»ÐºÐ° PSD-95 Ð² Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ°Ñ Ð² ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ð¸ Ñ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½Ð¾Ð¹ Ð³ÑÑÐ¿Ð¿Ð¾Ð¹, Ð½Ð¾ Ð½Ðµ Ð¸Ð·Ð¼ÐµÐ½ÑÐµÑ Ð¸Ñ ÑÐ°Ð·Ð¼ÐµÑ. ÐÐ¸ÑÑÑ-Ð¾Ð¿Ð¾ÑÑÐµÐ´Ð¾Ð²Ð°Ð½Ð½ÑÐ¹ Ð½Ð¾ÐºÐ´Ð°ÑÐ½ Ð±ÐµÐ»ÐºÐ° EB3 Ð¿ÑÐ¸Ð²Ð¾Ð´Ð¸Ñ Ðº Ð·Ð½Ð°ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ð¼Ñ ÑÐ¼ÐµÐ½ÑÑÐµÐ½Ð¸Ñ Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÐ¸ Ð¸ ÑÐ°Ð·Ð¼ÐµÑÐ° ÐºÐ»Ð°ÑÑÐµÑÐ¾Ð² Ð¿Ð¾ÑÑÑÐ¸Ð½Ð°Ð¿ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð±ÐµÐ»ÐºÐ° PSD-95. ÐÐ¸ÑÑÑ-Ð¾Ð¿Ð¾ÑÑÐµÐ´Ð¾Ð²Ð°Ð½Ð½Ð°Ñ Ð³Ð¸Ð¿ÐµÑÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð±ÐµÐ»ÐºÐ° EB3 Ð¿ÑÐ¸ Ð´Ð¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸Ð¸ ÑÐ¾ÐºÑÐ¸ÑÐ½ÑÑ Ð´Ð»Ñ Ð½ÐµÐ¹ÑÐ¾Ð½Ð¾Ð² Ð¾Ð»Ð¸Ð³Ð¾Ð¼ÐµÑÐ½ÑÑ ÑÐ¾ÑÐ¼ Ð±ÐµÑÐ°-Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð° Ð¿ÑÐµÐ´Ð¾ÑÐ²ÑÐ°ÑÐ°ÐµÑ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ðµ Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÐ¸ Ð¸ ÑÐ¸ÑÐ¸Ð½Ñ Ð³Ð¾Ð»Ð¾Ð²ÐºÐ¸ Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÑ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð², Ð° ÑÐ°ÐºÐ¶Ðµ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ðµ Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÐ¸ ÐºÐ»Ð°ÑÑÐµÑÐ¾Ð² Ð±ÐµÐ»ÐºÐ° PSD-95. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð¿Ð¾Ð´ÑÐ²ÐµÑÐ¶Ð´Ð°ÑÑ Ð³Ð¸Ð¿Ð¾ÑÐµÐ·Ñ Ð¾ ÑÐ¾Ð¼, ÑÑÐ¾ Ð±ÐµÐ»Ð¾Ðº EB3 ÐºÑÐ°Ð¹Ð½Ðµ Ð²Ð°Ð¶ÐµÐ½ Ð´Ð»Ñ ÑÑÐ°Ð±Ð¸Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð¿Ð¾ÑÑÑÐ¸Ð½Ð°ÑÐ¸ÑÐµÑÐºÐ¸Ñ ÐºÐ¾Ð½ÑÐ°ÐºÑÐ¾Ð² Ð² Ð½Ð¾ÑÐ¼Ðµ Ð¸ ÑÐ½Ð¸Ð¶Ð°ÐµÑ Ð´ÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸Ð²Ð½Ð¾Ðµ Ð¿ÑÐ¾ÑÐ²Ð»ÐµÐ½Ð¸Ñ Ð² Ð½ÐµÐ¹ÑÐ¾Ð½Ð°Ñ Ð² ÑÑÐ»Ð¾Ð²Ð¸ÑÑ Ð½Ð¸Ð·ÐºÐ¾Ð¹ Ð°Ð¼Ð¸Ð»Ð¾Ð¸Ð´Ð½Ð¾Ð¹ ÑÐ¾ÐºÑÐ¸ÑÐ½Ð¾ÑÑÐ¸.
description: The EB3 protein fastens to the positive end of growing neuronal microtubules and regulates their dynamics. It is known that hyperexpression of EB3 protein leads to a significant increase in mushroom spines to the dominant fraction in primary hippocampal neurons. This study examines the neuroprotective influence of EB3 protein on hippocampal neurons under conditions of low amyloid toxicity modeling Alzheimer's disease. PSD-95 protein clustering is connected with the stability of dendritic spines. The author developed a methodological approach to analyze the density and size of the PSD-95 protein clusters in neuronal dendrites using local binarization when the background correction and noise elimination in confocal images had been made. It was shown that overexpression of EB3 protein causes an increase in the head width of neuronal mushroom-shaped spines and the density of PSD-95 protein clusters in dendrites compared with the control group, although if does not change their size. Virus-mediated knockdown of EB3 protein leads to a significant reduction in the density and cluster size of the postsynaptic PSD-95 protein. Virus-mediated overexpression of EB3 protein prevents a decrease in the density and width of dendritic spines heads, as well as a decrease in the density of PSD-95 protein clusters, while toxic for neurons oligomeric forms of beta-amyloid are added. Due to results of the current study the hypothesis was confirmed. The EB3 protein is extremely important for postsynaptic contacts stabilization in normal conditions. The EB3 protein reduces the degenerative manifestations in neurons under conditions of low amyloid toxicity.