cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
DO ENSINO ON-LINE AO PRESENCIAL
Creators: name: Marques, Viviane Cristina
name: Viana, Helena Brandão
Publisher: name: Internet Latent Corpus Journal
Year: 2023

description: Resumo: Esse artigo tem por objetivo apresentar experiências educacionais utilizadas para a transição do on-line para o presencial utilizando diferentes propostas pedagógicas com as crianças da Educação Infantil. Os ambientes foram preparados para provocarem diversas sensações, um convite à exploração das crianças através de materiais e propostas visando estimular a curiosidade e participação, além da criatividade, da comunicação, do pensamento reflexivo, entre outras intenções pedagógicas. Os principais desafios foram desenvolver propostas de acordo com as diferentes etapas de desenvolvimento das crianças que retornaram às aulas presenciais depois de ficarem 18 meses no ensino on-line, por isso a necessidade de proporcionar momentos e espaços significativos. Os resultados alcançados foram importantes para o desenvolvimento das crianças.
description: Internet Latent Corpus Journal, vol. 13 n.º 1 (2023): REPENSANDO A TECNOLOGIA NA EDUCAÇÃO
Concept paper - For an integrated poverty and living conditions indicator system (IPOLIS) database
Creators: name: Gábos, A.
name: Kopasz, M.
Publisher: name: Zenodo
Year: 2014
Subjects: poverty
simulation tool
youth
elderly
children
vulnerability

description: Abstract: The paper aims at providing a general conceptual framework, as well as to set up a structure for the Integrated Poverty and Living Conditions Indicator System (IPOLIS). IPOLIS will be the core outcome of the InGRID project and will cover three easy-to-reach vulnerable groups: children, youth and older people. The IPOLIS is aimed to improve infrastructure for analysing and monitoring the situation of these vulnerable groups. It is conceived to serves as a resource for various user groups (researchers, policy makers at different levels, NGO experts, journalists, students, etc.) to (i) monitor the situation of children, youth and elderly in the field of poverty, living conditions and quality of life; (ii) observe relationships between indicators and to detect cross-country patterns according to selected measures. The theoretical framework of the IPOLIS is based on the concept of quality of life, considered to be a multi-dimensional concept comprising objective measures, and people’s perceptions of these factors (economic, social, etc.), that is, subjective measures of objective substances. In terms of indicator selection, IPOLIS will include objective measures as well as subjective indicators of objective circumstances.
 The structure of IPOLIS consist of six domains (or dimensions) of quality of life, including material living conditions, labour market attachment and work-life balance, education and training, health and risk behaviours, social connectedness and participation, physical environment and safety. Beyond these six domains, policy and context indicators have been added to the indicator set. This structure has been defined after a detailed overview of existing practices for the whole population, for children, for youth and for elderly.
 The concept paper of IPOLIS also defines general and age-group specific indicator selection criteria, discusses dilemmas of the indicator selection process, and defines overviews the available data infrastructure. IPOILIS is expected to be launched during the first half of year 2016.
Vaccination of Macaques against Pathogenic Simian Immunodeficiency Virus with Venezuelan Equine Encephalitis Virus Replicon Particles
Creators: name: Montefiori, D. C.
name: Caley, I. J.
name: Frelinger, J. A.
name: Davis, N. L.
name: McGrath, K. M.
name: Johnston, R. E.
name: Irlbeck, D. M.
name: Connell, M. J.
name: Betts, M. R.
name: Swanstrom, R.
name: Brown, K. W.
name: Johnson, P. R.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2000

description: Vaccine vectors derived from Venezuelan equine encephalitis virus (VEE) that expressed simian immunodeficiency virus (SIV) immunogens were tested in rhesus macaques as part of the effort to design a safe and effective vaccine for human immunodeficiency virus. Immunization with VEE replicon particles induced both humoral and cellular immune responses. Four of four vaccinated animals were protected against disease for at least 16 months following intravenous challenge with a pathogenic SIV swarm, while two of four controls required euthanasia at 10 and 11 weeks. Vaccination reduced the mean peak viral load 100-fold. The plasma viral load was reduced to below the limit of detection (1,500 genome copies/ml) in one vaccinated animal between 6 and 16 weeks postchallenge and in another from week 6 through the last sampling time (40 weeks postchallenge). The extent of reduction in challenge virus replication was directly correlated with the strength of the immune response induced by the vectors, which suggests that vaccination was effective.
From Fear to Self-Expression: The Contextual Nature of Physics Student Motivations
Creators: name: Van Dusen, Ben
name: Otero, Valerie
Publisher: name: arXiv
Year: 2014
Subjects: subject: Physics Education (physics.ed-ph)
subject: FOS: Physical sciences

description: This study utilizes a sociocultural interpretation of Self-Determination Theory to better understand the role that learning contexts play in generating student motivation, engagement, and identity. By drawing on previous motivation research we develop a model that describes how student senses of belonging in social settings can transform their goals and experiences. We use the extremes of fear and integrity to model student motivations to engage in activities. Student senses of connection and belonging (or not) in social settings drives whether they feel integrated with or alienated from their environments. Our model is based on three studies and suggests that a sense of belonging emerges through the alignment of goals and practices of the individual and an activity. This model is applied to two examples to illustrate how social connection or isolation can be exhibited in a physics classroom setting. We conclude by discussing the role of the teacher in designing classroom environments that support students engaging.
description: 4 pages, 2 figures, Physics Education Research Conference Proceedings 2014
INTELIGÊNCIA ARTIFICIAL, DISRUPÇÃO EDUCACIONAL E AS MARGARIDAS DO CAMPO
Creators: name: Vilela Jr., Guanis De Barros
name: Passos, Ricardo Pablo
Publisher: name: Internet Latent Corpus Journal
Year: 2023

description: : O fluxo das mudanças que revolucionaram a história da civilização não é linear, e provavelmente, é cada vez mais acelerado. Várias ferramentas de inteligência artificial já estão disponíveis também no ambiente educacional, onde algoritmos inteligentes são capazes de realizar com eficiência praticamente qualquer tarefa cognitiva; de um texto sobre filosofia da ciência ou mesmo programar um aplicativo de perguntas e respostas sobre cálculo vetorial. O objetivo deste artigo é refletir sobre os desafios que professores e gestores educacionais já estão enfrentando diante deste cenário disruptivo, fascinante para alguns, apavorante para outros. Ferramentas hoje já consolidadas em nossas vidas, causaram muito alvoroço e resistências quando foram desenvolvidas; foi o caso da eletricidade, do automóvel, da internet. Com a inteligência artificial não será diferente. Na educação existe uma evidente incoerência: de um lado, professores e pesquisadores reivindicam o protagonismo da vanguarda na consolidação de um mundo melhor através da construção do conhecimento. Por outro lado, são também subservientes ao modelo educacional hegemônico embolorado que teima em tentar uma sobrevida para um paradigma educacional que agoniza após mais de mil anos de sucesso inconteste. Professores e pesquisadores geradores de conhecimento serão cada vez mais importantes; professores replicadores de conhecimento, talvez tenham que buscar outros caminhos. A inteligência artificial não deve ser beatificada nem demonizada por nós, professores, afinal, é o uso que faremos dela, que definirá um espetacular avanço nas formas de educar ou, até mesmo, quem sabe, o nosso fim.
description: Internet Latent Corpus Journal, vol. 13 n.º 1 (2023): REPENSANDO A TECNOLOGIA NA EDUCAÇÃO
ALFABETIZAÇÃO EM TEMPOS DE PANDEMIA
Creators: name: Darius, Rebeca Pizza Pancotte
name: Fuckner, Malton De Oliveira
name: Goetz, Lisiane
name: Dos Santos, Maximiliana Batista Ferraz
Publisher: name: Internet Latent Corpus Journal
Year: 2023

description: Internet Latent Corpus Journal, vol. 13 n.º 1 (2023): REPENSANDO A TECNOLOGIA NA EDUCAÇÃO
title: Toll-like receptor 3 activation promotes joint degeneration in osteoarthritis
Creators: name: Stolberg-Stolberg, Josef
name: Boettcher, Annika
name: Sambale, Meike
name: Stuecker, Sina
name: Sherwood, Joanna
name: Raschke, Michael J.
name: Pap, Thomas
name: Bertrand, Jessica
Publisher: name: University of Muenster, Institutional Repository MIAMI
Year: 2023
Subjects: subject: 610 Medicine and health

description: Osteoarthritis (OA) is characterized by cartilage degradation that is induced by inflammation. Sterile inflammation can be caused by damage-associated molecular patterns that are released by chondrocytes and activate pattern recognition receptors. We evaluate the role of toll-like receptor-3-activating RNA in the pathogenesis of OA. Toll-like receptor 3 (TLR3) was detected by semiquantitative reverse transcriptase PCR, western blotting and microscopy. Rhodamine-labelled poly(I:C) was used to image uptake in chondrocytes and full-thickness cartilage. The production of IFNβ in chondrocytes after stimulation with poly(I:C) as well as in the synovial fluid of OA patients was measured using ELISA. Chondrocyte apoptosis was chemically induced using staurosporine. Immunohistochemistry was performed to examine TLR3 expression and apoptosis in human and murine OA cartilage. RNA in synovial fluid was quantified by RiboGreen assay. Destabilisation of the medial meniscus was performed in TLR3−/− and wildtype mice. OA was assessed after eight weeks using OARSI score. TLR3 expression was confirmed by western blot and RT-PCR. Poly(I:C) was internalised by chondrocytes as well as cartilage and caused an increase of IFNβ production in murine (11.46 ± 11.63 (wo) to 108.7 ± 25.53 pg/ml; N = 6) and human chondrocytes (1.88 ± 0.32 (wo) to 737.6 ± 130.5 pg/ml; N = 3; p < 0.001). OA cartilage showed significantly more TLR3-positive (KL0 = 0.22 ± 0.24; KL4 = 6.02 ± 6.75; N ≥ 15) and apoptotic chondrocytes (KL0 = 0.6 ± 1.02; KL4 = 9.78 ± 7.79; N ≥ 12) than healthy cartilage (p < 0.001). Staurosporine-induced chondrocyte apoptosis causes a dose-dependent RNA release (0 ng/ml = 1090 ± 39.1 ng/ml; 1000 ng/ml=2014 ± 160 ng/ml; N = 4; p < 0.001). Human OA synovial fluid contained increased concentrations of RNA (KL0-2 = 3408 ± 1129 ng/ml; KL4 = 4870 ± 1612ng/ml; N ≥ 7; p < 0.05) and IFNβ (KL0-2 = 41.95 ± 92.94 ng/ml; KL3 = 1181 ± 1865ng/ml; N ≥ 8; p < 0.05). TLR3−/− mice showed reduced cartilage degradation eight weeks after OA induction (OARSI WT = 5.5 ± 0.04; TLR3−/− = 3.75 ± 1.04; N ≥ 6) which was accompanied by gradually decreasing levels of TUNEL-positive cells (WT = 34.87 ± 24.10; TLR3−/ = 19.64 ± 7.89) resulting in decreased IFNβ expression (WT = 12.57 ± 5.43; TLR3−/− = 6.09 ± 2.07) in cartilage (p < 0.05). The release of RNA by apoptotic chondrocytes thus activating TLR3 signalling is one possible way of perpetuating inflammatory cartilage changes. The inhibition of TLR3 could be a possible therapeutic target for OA treatment.
description: Finanziert über die DEAL-Vereinbarung mit Wiley 2019-2022.
O ENSINO DE ARTE, TECNOLOGIAS EDUCACIONAIS E A PROPOSTA REFLEXIVA SOBRE AS TRIBOS URBANAS
Creators: name: Matusaiki, Cinthia Caciele Fregne
name: Rocha Prates, Eli Andrade Da
name: Viana, Helena Brandão
Publisher: name: Internet Latent Corpus Journal
Year: 2023

description: Com o objetivo de discutir, dentro do ambiente escolar, questões sobre Arte, tecnologias e ferramentas educacionais, bem como suas relações com Tribos Urbanas, a produção e execução de uma oficina de aprendizagem com essa temática possibilitou a concretização de pesquisas e ampliação de conceitos a respeito da diversidade social e cultural existente em nossa sociedade. Estamos inseridos em ambientes em que há recorrentes casos de intolerância, desrespeito e falta de empatia, agravados pelo mau uso das tecnologias pelos nossos jovens e adolescentes. Nesse sentido, enxergamos a educação como uma ferramenta capaz de estreitar laços e despertar conhecimentos a fim de proporcionar uma sociedade justa e igualitária por meio de tecnologias e ferramentas digitais.
description: Internet Latent Corpus Journal, vol. 13 n.º 1 (2023): REPENSANDO A TECNOLOGIA NA EDUCAÇÃO
An Epstein-Barr Virus Protein Interacts with Notch
Creators: name: Raab-Traub, N.
name: Kusano, S.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2001

description: The Epstein-Barr virus (EBV) BamHI A mRNAs were originally identified in cDNA libraries from nasopharyngeal carcinoma, where they are expressed at high levels. The RNAs are differentially spliced to form several open reading frames and also contain the BARF0 open reading frame at the 3′ end. One cDNA, RK-BARF0, included a potential endoplasmic reticulum-targeting signal peptide sequence. The RK-BARF0 protein is shown here to interact with the Notch4 ligand binding domain, using yeast two-hybrid screening, coimmunoprecipitation, and confocal microscopy. This interaction induces translocation of a portion of the full-length unprocessed Notch4 to the nucleus by using the Notch nuclear localization signal. These effects of RK-BARF0 on Notch intracellular location indicate that EBV possibly modulates Notch signaling. Unprocessed Notch4 was also detected in immunoprecipitated complexes from EBV-infected cells by using a rabbit antiserum raised against a BARF0-specific peptide. This finding provides additional evidence for expression of RK-BARF0 and its interaction with Notch during EBV infection. In EBV-infected, EBNA2-negative cells, RK-BARF0 induced the expression of EBV latent membrane protein 1 (LMP1), and this induction was dependent on the RK-BARF0/Notch interaction domain. The activation of LMP1 expression by RK-BARF0 may be responsible for expression of LMP1 in EBV latent infections in the absence of EBNA2.
Analysis of a chaotic spiking neural model: The NDS neuron
Creators: name: Alhawarat, Mohammad
name: Nazih, Waleed
name: Eldesouki, Mohammad
Publisher: name: arXiv
Year: 2014
Subjects: subject: Neural and Evolutionary Computing (cs.NE)
subject: Chaotic Dynamics (nlin.CD)
subject: FOS: Computer and information sciences
subject: FOS: Physical sciences

description: Further analysis and experimentation is carried out in this paper for a chaotic dynamic model, viz. the Nonlinear Dynamic State neuron (NDS). The analysis and experimentations are performed to further understand the underlying dynamics of the model and enhance it as well. Chaos provides many interesting properties that can be exploited to achieve computational tasks. Such properties are sensitivity to initial conditions, space filling, control and synchronization.Chaos might play an important role in information processing tasks in human brain as suggested by biologists. If artificial neural networks (ANNs) is equipped with chaos then it will enrich the dynamic behaviours of such networks. The NDS model has some limitations and can be overcome in different ways. In this paper different approaches are followed to push the boundaries of the NDS model in order to enhance it. One way is to study the effects of scaling the parameters of the chaotic equations of the NDS model and study the resulted dynamics. Another way is to study the method that is used in discretization of the original Rössler that the NDS model is based on. These approaches have revealed some facts about the NDS attractor and suggest why such a model can be stabilized to large number of unstable periodic orbits (UPOs) which might correspond to memories in phase space.
description: Computer Science & Information Technology, 2013, Vol. 4 No. 3
Characterization of Human Immunodeficiency Virus Type 1 in Saliva and Blood Plasma by V3-Specific Heteroduplex Tracking Assay and Genotype Analyses
Creators: name: Patton, L. L.
name: Swanstrom, R.
name: Fiscus, S. A.
name: Nelson, J. A. E.
name: Shugars, D. C.
name: Williams, J. M.
name: Freel, S. A.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2001

description: The gp120 V3-encoding region of human immunodeficiency virus type 1 (HIV-1) RNA derived from the saliva and blood plasma of 11 individuals was characterized by heteroduplex tracking assay and sequence analyses. R5-like viral variants were identified in both fluids of all subjects. X4-like variants were detected in the plasma and/or saliva of three subjects, indicating that X4-like variants are not excluded from the saliva compartment. Viral subpopulations were similar in both fluids of most subjects, suggesting that HIV-1 in oral fluids and blood may stem from a common source. These findings raise the possibility of using saliva as a noninvasive fluid for evaluating and monitoring viral evolution in infected persons.
MULTIMEDIA RESOURCES IN THE PROCESS OF THE LEARNING - AN EXPERIENCE REPORT
Creators: name: Quadros, Sílvia Cristina De Oliveira
name: Salomão, Lia Santos De Oliveira
Publisher: name: Internet Latent Corpus Journal
Year: 2023

description: Este estudo consiste em um relato de experiência com o uso da plataforma M3, desenvolvida pela Universidade de Campinas, e aplicada em uma escola estadual de educação básica, de ensino integral (PEI), localizada na zona sul da cidade de São Paulo. O objetivo foi analisar de que forma os recursos tecnológicos disponíveis na plataforma pôde auxiliar na melhoria dos resultados dos alunos no desempenho na matemática no programa de nivelamento. Para verificar se houve consolidação das habilidades trabalhadas, foi analisado o resultado no desempenho em Matemática na avaliação do Sistema de Avaliação do Rendimento Escolar e, de posse dos indicadores da Avaliação de Aprendizagem em Processo, as plataformas de educação a distância foram utilizadas como meio para que a aprendizagem fosse desenvolvida através do uso da plataforma M3. A avaliação da plataforma foi realizada por dois docentes que trabalhavam no Ensino Médio com a disciplina de Matemática, sob os aspectos: (praticidade, conteúdo, pedagógico e linguagem). Os resultados obtidos demonstraram que a maioria das perguntas estavam classificadas no quase sempre, apontando um bom desempenho do software. Com o uso da plataforma de educação a distância verificou-se que o número de alunos classificados no nível abaixo do básico diminuiu a cada edição da Avaliação de Aprendizagem em Processo: 15ª. edição (48,5%), 16ª. edição (40,8%) e na 17ª edição (29,2%). Os resultados esperados foram alcançados. Conclui-se que o uso da plataforma, sob uma ótica de desenvolvimento, interação e impacto nos resultados frente aos objetivos pedagógicos traçados foram bem-sucedidos.
description: Internet Latent Corpus Journal, vol. 13 n.º 1 (2023): REPENSANDO A TECNOLOGIA NA EDUCAÇÃO
Determinants of working conditions and occupational health and safety conditions connected with economic change
Creators: name: Beblavý, B.
name: Akgüç, M.
name: Fabo, B.
name: Lenaerts, K.
name: Paquier, F.
Publisher: name: Zenodo
Year: 2017
Subjects: working conditions
occupational safety and health
economic change

description: Abstract: In recent years, there have been widespread debates on the ‘future of work’ in Europe, as megatrends, such as technological change, globalisation and ageing of the population, have transformed countless jobs - which in turn calls for new skills. At the same time, many European countries are still recov­ering from the Great Recession. With the recent upsurge of unemployment still in mind, European citizens have raised concerns about what will happen to their job. It is against this background that this report explores the dynamics of new or emerging occupations, jobs, tasks and skills, and their identification in particular. To this end, the report starts with an extensive review of the literature, in order to reveal how these concepts are defined and measured. It then continues with an assessment of the strengths and limitations of the most commonly used methodologies and data sets and presents alternatives to both. Our focus is on the potential of web data from different sources, ranging from social media, over surveys, to online job boards and vacancies. The final chapters of the report pre­sent the results from a series of pilots that we carried out, each based on web data, with the objective to further our understanding of new occupations and skills. The report is concluded with a chapter that highlights one of these pilots; it examines skill mismatches by linking educational requirements from online vacancies to educational attainments of jobholders. We conclude that web data have much potential in this research area and can be highly valuable to provide policymakers with real-time information on ongoing trends.
Enhancement of Adenovirus Vector Entry into CD70-Positive B-Cell Lines by Using a Bispecific CD70-Adenovirus Fiber Antibody
Creators: name: Israel, B. F.
name: Kenney, S. C.
name: Gerard, R. D.
name: Pickles, R. J.
name: Segal, D. M.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2001

description: Although many recombinant adenovirus vectors (rAd) have been developed, especially by using group C adenoviruses, to transfer and express genes, such rAd do not readily infect B-cell lines due to the lack of the coxsackievirus-adenovirus receptor. Bispecific antibodies have been used in different cell systems to facilitate entry of rAd into otherwise nonpermissive cells. Bispecific antibody is synthesized by covalently linking two monoclonal antibodies with distinct specificities. It has been shown that lymphoproliferative tumors commonly express the cell surface protein CD70, while this receptor is normally expressed on only a small subset of highly activated B cells and T cells. We therefore investigated whether a bispecific antibody with specificities for the adenovirus fiber protein and CD70 can facilitate rAd entry and subsequent expression of rAd-encoded genes in CD70-positive B cells. We found high CD70 expression on Epstein-Barr virus (EBV)-transformed lymphoblastoid cell lines (LCLs), as well as some, but not all, Burkitt lymphoma (BL) lines. We show here that rAd encoding green fluorescent protein (Ad-GFP) infects EBV-transformed LCLs and a CD70-positive BL line 10- to 20-fold more efficiently in the presence of the CD70-fiber bispecific antibody. In contrast, the bispecific antibody does not enhance Ad-GFP infection in CD70-deficient BL cells. Using the CD70-fiber bispecific antibody, we increased the ability of rAd vectors encoding the EBV immediate-early proteins BZLF1 and BRLF1 to induce the lytic form of EBV infection in LCLs. These results indicate that the CD70-fiber bispecific antibody can enhance rAd infection of CD70-positive B cells and suggest the use of this vector to explore EBV-positive LCLs.
Epstein-Barr Virus Immediate-Early Protein BRLF1 Induces the Lytic Form of Viral Replication through a Mechanism Involving Phosphatidylinositol-3 Kinase Activation
Creators: name: Kenney, S.
name: Mauser, A.
name: Darr, C. D.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2001

description: Expression of the Epstein-Barr virus (EBV) immediate-early (IE) protein BRLF1 induces the lytic form of viral replication in most EBV-positive cell lines. BRLF1 is a transcriptional activator that binds directly to a GC-rich motif present in some EBV lytic gene promoters. However, BRLF1 activates transcription of the other IE protein, BZLF1, through an indirect mechanism which we previously showed to require activation of the stress mitogen-activated protein kinases. Here we demonstrate that BRLF1 activates phosphatidylinositol-3 (PI3) kinase signaling in host cells. We show that the specific PI3 kinase inhibitor, LY294002, completely abrogates the ability of a BRLF1 adenovirus vector to induce the lytic form of EBV infection, while not affecting lytic infection induced by a BZLF1 adenovirus vector. Furthermore, we demonstrate that the requirement for PI3 kinase activation in BRLF1-induced transcriptional activation is promoter dependent. BRLF1 activation of the SM early promoter (which occurs through a direct binding mechanism) does not require PI3 kinase activation, whereas activation of the IE BZLF1 and early BMRF1 promoters requires PI3 kinase activation. Thus, there are clearly two separate mechanisms by which BRLF1 induces transcriptional activation.
title: Effect of Thermo-Mechanically Activated Precipitation on the Hot Deformation Behavior of High Strength Aluminum Alloy AA7075
Creators: name: Scharifi, Emad
name: Nietsch, Jürgen A.
name: Quadfasel, Angela
name: Weidig, Ursula
name: Steinhoff, Kurt
Publisher: name: Universität Kassel
Year: 2022
Subjects: subject: hot deformation
subject: dynamic precipitation
subject: thermo-mechanical softening mechanisms
subject: 620
subject: Aluminiumlegierung
subject: Thermomechanik
subject: Deformation
subject: Entfestigung
subject: Dehnung
subject: Fließverhalten
subject: Temperaturverhalten

description: Gefördert durch den Publikationsfonds der Universität Kassel
Alternative splicing of transposable elements in human breast cancer
Creators: name: Nesta, Alex
Publisher: name: Zenodo
Year: 2024

description: Transposable elements (TEs) are an important driver of genome evolution and can affect gene expression through diverse mechanisms. In breast cancer, disrupted TE sequence regulation may facilitate tumor-specific transcriptomic alterations. Our study utilized long-read RNA sequencing (LR-seq) from 30 breast samples (142,514 full-length isoforms) to examine the effects of TEs on the breast cancer transcriptome. We identified hundreds of unique transcription start sites, splice sites, and termination sites within or adjacent to TEs. Our examination of TEs in the context of post-transcriptionalRNA editing revealed thousands of isoforms with editing signatures, including a novel RHOA isoform implicated in lung tumor progression. Polymorphic TE insertions are a source of genomic variation amongst individuals and have the potential to alter splicing.The full-length isoforms captured by long-reads enabled us to identify spliced TEs that are unique to individuals with the insertion. Together, our results demonstrate the widespread effects of dysregulated TEs on breast cancer transcriptomes and the advantages of long-read isoform sequencing for understanding TE biology. TE isoforms may alter the expression of genes important in cancer and could potentially be used as novel, disease-specific, therapeutic targets.
Epstein-Barr Virus Immediate-Early Protein BRLF1 Interacts with CBP, Promoting Enhanced BRLF1 Transactivation
Creators: name: Kenney, S. C.
name: Holley-Guthrie, E.
name: Swenson, J. J.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2001

description: The Epstein-Barr virus (EBV) immediate-early protein BRLF1 is a transcriptional activator that mediates the switch from latent to lytic viral replication. Many transcriptional activators function, in part, due to an interaction with histone acetylases, such as CREB-binding protein (CBP). Here we demonstrate that BRLF1 interacts with the amino and carboxy termini of CBP and that multiple domains of the BRLF1 protein are necessary for this interaction. Furthermore, we show that the interaction between BRLF1 and CBP is important for BRLF1-induced activation of the early lytic EBV gene SM in Raji cells.
Job quality and vulnerability-at-work indicators in Europe
Creators: name: Greenan, N.
name: Seghir, S.
name: Smits, I.
name: Szekér, L.
name: Van Gyes, G.
name: Vercruyssen, A.
Publisher: name: Zenodo
Year: 2017
Subjects: job quality
vulnerability
work

description: Abstract: In the harsh new world of global competition and in the face of new wave of technological innova­tion, the world of work seems to undertake continuous changes. Further, structural transformations of the economy induced and extended by economic crisis as well as demographic and labour market changes imply the emergence of new forms of employment. However, there are also risks involved, in particular an increase of more precarious jobs and of inequality within and across countries that jeopardise the individual well-being. Against this backdrop, this report is put forth to highlight the global changes of job quality and work-related vulnerabilities in European countries, developing methods to identify the most at-risk employees, and also providing improvement guides of job quali­ty. To this end, the first chapter presents a job-centred methodology aiming to combine the multiples dimensions of jobs into reduced number of job types. The output is a taxonomy of job types that opens room for job quality evaluation and improvement. The second chapter provides an employee-centred method to measure vulnerability to adverse working environment. The main outcome is a vulnerability measure comparable across European countries and a warning system of employees at risk. Finally, the third chapter provides an assessment of the possibility of an international standard for scale construction using ECWS 2010.
Synthesizing Lattice Structure in Phase Space
Creators: name: Guo, Lingzhen
name: Marthaler, Michael
Publisher: name: arXiv
Year: 2014
Subjects: subject: Quantum Gases (cond-mat.quant-gas)
subject: Mesoscale and Nanoscale Physics (cond-mat.mes-hall)
subject: Quantum Physics (quant-ph)
subject: FOS: Physical sciences

description: We consider a realistic model, i.e., ultracold atoms in a driven optical lattice, to realize phase space crystals [Phys. Rev. Lett. 111, 205303 (2013)]. The corresponding lattice structure in phase space is more complex and contains rich physics. A phase space lattice differs fundamentally from a lattice in real space, because its coordinate system, i.e., phase space, has a noncommutative geometry, which naturally provides an artificial gauge (magnetic) field. We study the behavior of the quasienergy band structure as function of the artificial magnetic field and investigate the thermal properties. Synthesizing lattice structures in phase space is not only a new way to create artificial lattice in experiments but also provides a platform to study the intriguing phenomena of driven systems far away from equilibrium.
description: 11 pages, 6 figures
Stress reorganisation and response in active solids
Creators: name: Hawkins, Rhoda J.
name: Liverpool, Tanniemola B.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Soft Condensed Matter (cond-mat.soft)
subject: Biological Physics (physics.bio-ph)
subject: Cell Behavior (q-bio.CB)
subject: FOS: Physical sciences
subject: FOS: Biological sciences

description: We present a microscopic model of a disordered viscoelastic active solid, i.e. an active material whose long time behaviour is elastic as opposed to viscous. It is composed of filaments, passive crosslinks and molecular motors powered by stored chemical energy, e.g. actomyosin powered by ATP. Our model allows us to study the collective behaviour of contractile active elements and how their interaction with each other and the passive elastic elements determines the macroscopic mechanical properties of the active material. As a result of the (un)binding dynamics of the active elements, we find that this system provides a highly responsive material with a dynamic mechanical response strongly dependent on the amount of deformation.
description: 5 pages, 2 figures, Published 7 July 2014
USOS DE MATERIAIS CONCRETOS E DISPOSITIVOS MÓVEIS COM UMA ALUNA COM DEFICIÊNCIA VISUAL
Creators: name: Wataya, Roberto Sussumu
name: Do Prado, Ivanildo Gomes
name: Viana, Helena Brandão
Publisher: name: Internet Latent Corpus Journal
Year: 2023

description: Este estudo busca apresentar, uma experiência pontual de inclusão de alunos com deficiência visual, a partir dos recursos e dos impactos das tecnologias visando minimizar as deficiências, e promovendo uma ação efetiva no processo de aprendizagem. O objetivo desta pesquisa foi apresentar os recursos que podem ser utilizados por uma aluna com deficiência visual para o ensino de figuras geométricas. Iniciou-se com a pesquisa bibliográfica nas bases de dados científicas na perspectiva de conhecer os currículos programáticos e a utilização das tecnologias, em escolas especializadas para alunos com deficiência visual. O estudo lança “desafios de elucidar” o quanto as utilizações das tecnologias favorecem alunos(as) com deficiência visual e aos professores(as), beneficiando os envolvidos de forma significativa e o processo ensino aprendizagem. O resultado mostrou ser possível a aprendizagem de conceitos e conteúdos matemáticos, neste caso “figuras geométricas”, por indivíduos com deficiência visual, desde que planejadas adequadamente as aulas com os usos das tecnologias, pois tais recursos podem promover aos alunos(as) com deficiências visuais, superarem as barreiras impostas pela cegueira.
description: Internet Latent Corpus Journal, vol. 13 n.º 1 (2023): REPENSANDO A TECNOLOGIA NA EDUCAÇÃO
Epstein-Barr Virus Immediate-Early Protein BZLF1 Is SUMO-1 Modified and Disrupts Promyelocytic Leukemia Bodies
Creators: name: Adamson, A. L.
name: Kenney, S.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2001

description: Although the immediate-early proteins of both herpes simplex virus (HSV) and cytomegalovirus (CMV) are known to modify promyelocytic leukemia (PML) (ND10) bodies in the nucleus of the host cell, it has been unclear whether lytic infection with gamma herpesviruses induces a similar effect. The PML protein is induced by interferon, involved in major histocompatibility complex class I presentation, and necessary for certain types of apoptosis. Therefore, it is likely that PML bodies function in an antiviral capacity. SUMO-1 modification of PML is known to be required for the formation of PML bodies. To examine whether Epstein-Barr virus (EBV) lytic replication interferes with PML bodies, we expressed the EBV immediate-early genes BZLF1 (Z) and BRLF1 (R) in EBV-positive cell lines and examined PML localization. Both Z and R expression resulted in PML dispersion in EBV-positive cells. Z but not R expression is sufficient to disrupt PML bodies in EBV-negative cell lines. We show that dispersion of PML bodies by Z requires a portion of the transcriptional activation domain of Z but not the DNA-binding function. As was previously reported for the HSV-1 ICP0 and CMV IE1 proteins, Z reduces the amount of SUMO-1-modified PML. We also found that Z itself is SUMO-1 modified (through amino acid 12) and that Z competes with PML for limiting amounts of SUMO-1. These results suggest that disruption of PML bodies is important for efficient lytic replication of EBV. Furthermore, Z may potentially alter the function of a variety of cellular proteins by inhibiting SUMO-1 modification.
Reexamining classical and quantum models for the D-Wave One processor
Creators: name: Albash, Tameem
name: Rønnow, Troels F.
name: Troyer, Matthias
name: Lidar, Daniel A.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Quantum Physics (quant-ph)
subject: FOS: Physical sciences

description: We revisit the evidence for quantum annealing in the D-Wave One device (DW1) based on the study of random Ising instances. Using the probability distributions of finding the ground states of such instances, previous work found agreement with both simulated quantum annealing (SQA) and a classical rotor model. Thus the DW1 ground state success probabilities are consistent with both models, and a different measure is needed to distinguish the data and the models. Here we consider measures that account for ground state degeneracy and the distributions of excited states, and present evidence that for these new measures neither SQA nor the classical rotor model correlate perfectly with the DW1 experiments. We thus provide evidence that SQA and the classical rotor model, both of which are classically efficient algorithms, do not satisfactorily explain all the DW1 data. A complete model for the DW1 remains an open problem. Using the same criteria we find that, on the other hand, SQA and the classical rotor model correlate closely with each other. To explain this we show that the rotor model can be derived as the semiclassical limit of the spin-coherent states path integral. We also find differences in which set of ground states is found by each method, though this feature is sensitive to calibration errors of the DW1 device and to simulation parameters.
description: 18 pages, 9 figures
O PENSAMENTO COMPUTACIONAL NA EDUCAÇÃO INFANTIL POR MEIO DE JOGOS
Creators: name: Marques, Viviane Cristina
name: Gamez, Luciano
Publisher: name: Internet Latent Corpus Journal
Year: 2023

description: Neste artigo aborda-se como introduzir o Pensamento Computacional na educação infantil por meio de jogos. Para a utilização de tais jogos, torna-se fundamental pensar a relação entre jogos e educação e, sobretudo, a associação entre jogos e brincadeiras para esta faixa etária. Partimos da necessidade de uma educação infantil reflexiva e com possibilidades de construção de conhecimentos, a qual permita às crianças terem experiências significativas com atividades lúdicas voltadas ao desenvolvimento do Pensamento Computacional.
description: Internet Latent Corpus Journal, vol. 13 n.º 1 (2023): REPENSANDO A TECNOLOGIA NA EDUCAÇÃO
Regression-free Synthesis for Concurrency
Creators: name: Černý, Pavol
name: Henzinger, Thomas A.
name: Radhakrishna, Arjun
name: Ryzhyk, Leonid
name: Tarrach, Thorsten
Publisher: name: arXiv
Year: 2014
Subjects: subject: Programming Languages (cs.PL)
subject: Logic in Computer Science (cs.LO)
subject: FOS: Computer and information sciences

description: While fixing concurrency bugs, program repair algorithms may introduce new concurrency bugs. We present an algorithm that avoids such regressions. The solution space is given by a set of program transformations we consider in for repair process. These include reordering of instructions within a thread and inserting atomic sections. The new algorithm learns a constraint on the space of candidate solutions, from both positive examples (error-free traces) and counterexamples (error traces). From each counterexample, the algorithm learns a constraint necessary to remove the errors. From each positive examples, it learns a constraint that is necessary in order to prevent the repair from turning the trace into an error trace. We implemented the algorithm and evaluated it on simplified Linux device drivers with known bugs.
description: for source code see https://github.com/thorstent/ConRepair
Human Cytomegalovirus Up-Regulates the Phosphatidylinositol 3-Kinase (PI3-K) Pathway: Inhibition of PI3-K Activity Inhibits Viral Replication and Virus-Induced Signaling
Creators: name: Huong, S.-M.
name: Ma, X.-L.
name: Johnson, R. A.
name: Huang, E.-S.
name: Wang, X.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2001

description: Infection of quiescent fibroblasts with human cytomegalovirus (HCMV) was found to cause a rapid activation of cellular phosphatidylinositol 3-kinase (PI3-K). Maximum PI3-K activation occurred from 15 to 30 min postinfection. This activation was transient, and by 2 h postinfection (hpi), PI3-K activity had declined to preinfection levels. However, at 4 hpi, a second tier of PI3-K activation was detected, and PI3-K activity remained elevated relative to that of mock-infected cells for the remainder of infection. The cellular kinases Akt and p70S6K and the transcription factor NF-κB were activated in a PI3-K-dependent manner at similar times following HCMV infection. Analysis using UV-irradiated virus indicated that no viral protein synthesis was necessary for the first phase of PI3-K activation, but viral protein expression was required for the second tier of PI3-K activation. Treatment of infected fibroblasts with LY294002, a potent and specific inhibitor of PI3-K kinase activity, caused a 4-log decrease in viral titers. LY294002 did not inhibit viral entry, but it did decrease viral immediate-early gene expression. In addition, the protein levels of two viral early genes required for DNA replication, UL84 and UL44, were significantly lower in the presence of LY294002. Furthermore, viral DNA replication was strongly inhibited by LY294002 treatment. This inhibition of viral DNA replication could be reversed by adding back the products of PI3-K activity (PI-3,4-P2 and PI-3,4,5-P3), demonstrating that the effect of LY294002 on the viral life cycle was specifically due to the inhibition of PI3-K activity. These results are the first to suggest that PI3-K mediates HCMV-induced activation of host cell mitogenic pathways. They also provide strong evidence that PI3-K activation is important for initiation of viral DNA replication and completion of the viral lytic life cycle.
DESENVOLVIMENTO DE UM INDICADOR DE APLICAÇÃO DAS TECNOLOGIAS PARA INSTITUIÇÕES EDUCACIONAIS
Creators: name: Viana, Helena Brandão
name: Gonçalves, Cláudio Xavier
Publisher: name: Internet Latent Corpus Journal
Year: 2023

description: os objetivos deste trabalho foram: criar um indicador para identificar o uso das Tecnologias de Informação e Comunicação na educação básica em uma rede privada de ensino; aplicar um instrumento de pesquisa para o levantamento do uso das TIC com gestores, professores e alunos; analisar, pelos indicadores, o nível de uso das TIC nas escolas pesquisadas. Foi efetuado um levantamento da utilização das TIC no ambiente educacional por meio da aplicação de questionários, aplicados a 5 Diretores, 57 Professores e 413 alunos de cinco escolas privadas de ensino médio no Estado de São Paulo. As informações foram coletadas, tabuladas e submetidas à análise fatorial, com o auxílio do software SPSS, para definição das dimensões específicas das aplicações de TIC, o que possibilitou a classificação das instituições pesquisadas no tocante aos escores obtidos em cada dimensão. Com estas informações foi criado o indicador de uso das TIC em cada instituição. Este indicador possibilitará que o gestor avalie quais dimensões atreladas às variáveis correspondentes estão proporcionando o desvio da aplicação de TIC do ideal esperado (0% - não aplicação de TIC, 100% - completa utilização de TIC), para que ações possam ser especificamente elaboradas para maximizar, valor do indicador o mais próximo de 100%, o uso das TIC. As escolas pesquisadas apresentaram indicadores de uso de TIC semelhantes, porém com percepção diferenciada dos gestores, que foi mais otimista em relação à percepção dos professores e dos alunos, relativas a aplicação de TIC nas instituições.
description: Internet Latent Corpus Journal, vol. 13 n.º 1 (2023): REPENSANDO A TECNOLOGIA NA EDUCAÇÃO
PT-symmetric interpretation of the electromagnetic self-force
Creators: name: Bender, Carl M.
name: Gianfreda, Mariagiovanna
Publisher: name: arXiv
Year: 2014
Subjects: subject: High Energy Physics - Theory (hep-th)
subject: Mathematical Physics (math-ph)
subject: Quantum Physics (quant-ph)
subject: FOS: Physical sciences

description: In 1980 Englert examined the classic problem of the electromagnetic self-force on an oscillating charged particle. His approach, which was based on an earlier idea of Bateman, was to introduce a charge-conjugate particle and to show that the two-particle system is Hamiltonian. Unfortunately, Englert's model did not solve the problem of runaway modes, and the corresponding quantum theory had ghost states. It is shown here that Englert's Hamiltonian is PT symmetric, and that the problems with his model arise because the PT symmetry is broken at both the classical and quantum level. However, by allowing the charged particles to interact and by adjusting the coupling parameters to put the model into an unbroken PT-symmetric region, one eliminates the classical runaway modes and obtains a corresponding quantum system that is ghost free.
description: 4 pages, 2 figures
Resolving the puzzling decay patterns of charged $Z_c$ and $Z_b$ states
Creators: name: Liu, Xiao-Hai
name: Ma, Li
name: Sun, Li-Ping
name: Liu, Xiang
name: Zhu, Shi-Lin
Publisher: name: arXiv
Year: 2014
Subjects: subject: High Energy Physics - Phenomenology (hep-ph)
subject: High Energy Physics - Experiment (hep-ex)
subject: Nuclear Theory (nucl-th)
subject: FOS: Physical sciences

description: We investigate the ratio of the branching fractions of the molecular candidates decaying into the ground and radially excited states within the quark interchange model. Our numerical results suggest that these molecular candidates are more likely to decay into the radially excited states than into ground states. Especially, the ratio $Γ[Z_c(4430)\to πψ(2S)]$/$Γ[Z_c(4430)\to πJ/ψ]\sim 9.8$ is close to the experimental measurement, which supports the interpretation of $Z_c(4430)$ as the $\bar{D}D^*(2S)$ molecular state. The ratios of the branching fractions of $Z_b(10610)$ and $Z_b(10650)$ to $πΥ(2S, 3S)$ and $πΥ(1S)$ agrees very well with Belle's measurement. We also predict the similar ratios for $Z_c(3900)$, $Z_c(4020)$, $R_{Z_c(3900)}$$\approx$1.3 and $R_{Z_c(4020)}$$\approx$$4.7$. Hopefully the $πψ(2S)$ mode, and ratios $R_{Z_c(3900)}$ and $R_{Z_c(4020)}$ will be measured by the BESIII and Belle collaborations in the near future, which shall be very helpful to understand the underlying dynamics of these exotic states.
description: 8 pages, 5 figures, 4 tables
Vector form factor of the pion in chiral effective field theory
Creators: name: Djukanovic, D.
name: Gegelia, J.
name: Keller, A.
name: Scherer, S.
name: Tiator, L.
Publisher: name: arXiv
Year: 2014
Subjects: subject: High Energy Physics - Phenomenology (hep-ph)
subject: Nuclear Theory (nucl-th)
subject: FOS: Physical sciences

description: The vector form factor of the pion is calculated in the framework of chiral effective field theory with vector mesons included as dynamical degrees of freedom. To construct an effective field theory with a consistent power counting, the complex-mass scheme is applied.
description: 11 pages, 2 figures