title: ÐÐ½ÐµÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐ¹ Ð¼Ð°ÑÑÐ¸ÐºÑ ÐºÐ°Ðº Ð¾ÑÐ½Ð¾Ð²Ð½Ð°Ñ Ð¾Ð¿ÑÑ Ð¾Ð»ÐµÐ²Ð°Ñ Ð½Ð¸ÑÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: The extracellular matrix as the main tumor niche
Creators: name: ÐÐ²ÐµÑÐ¸Ð½Ð°, Ð¢Ð°ÑÑÑÐ½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð¼Ð¸ÐºÑÐ¾Ð¾ÐºÑÑÐ¶ÐµÐ½Ð¸Ðµ Ð¾Ð¿ÑÑ Ð¾Ð»Ð¸
Ð¾Ð¿ÑÑ Ð¾Ð»ÐµÐ²ÑÐ¹ Ð²Ð½ÐµÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐ¹ Ð¼Ð°ÑÑÐ¸ÐºÑ
Ð¾Ð¿ÑÑ Ð¾Ð»ÐµÐ²ÑÐµ ÐºÐ»ÐµÑÐºÐ¸
Ð¾ÑÑÐµÐ¾ÑÐ°ÑÐºÐ¾Ð¼Ð°
Ð°Ð½Ð³Ð¸Ð¾Ð³ÐµÐ½ÐµÐ·
Ð¼Ð¸Ð³ÑÐ°ÑÐ¸Ñ
Ð¿ÑÐ¾Ð³ÑÐµÑÑÐ¸Ñ
ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾ÑÑÑ ÐºÐ»ÐµÑÐ¾Ðº
tumor microenvironment
tumor extracellular matrix
tumor cells
osteosarcoma
cell angiogenesis
migration
progression
resistance

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¸Ñ ÑÐ¾Ð»Ð¸ Ð²Ð½ÐµÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ Ð¼Ð°ÑÑÐ¸ÐºÑÐ° ÐºÐ°Ðº ÑÐ¾ÑÑÐ°Ð²Ð»ÑÑÑÐµÐ¹ Ð¼Ð¸ÐºÑÐ¾Ð¾ÐºÑÑÐ¶ÐµÐ½Ð¸Ñ Ð¾Ð¿ÑÑÐ¾Ð»ÐµÐ²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð½Ð° Ð¸Ñ Ð¿ÑÐ¾Ð³ÑÐµÑÑÐ¸Ñ. Ð ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ» Ð¾ÑÑÐ°Ð±Ð¾ÑÐ°Ð½ Ð¿ÑÐ¾ÑÐ¾ÐºÐ¾Ð» Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ Ð²Ð½ÐµÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ Ð¼Ð°ÑÑÐ¸ÐºÑÐ° ÑÐ°ÐºÐ¾Ð²ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð¾ÑÑÐµÐ¾ÑÐ°ÑÐºÐ¾Ð¼Ñ. Ð¡ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÐÐ¦Ð -ÑÐµÑÑÐ¾Ð² Ð² ÑÐµÐ¶Ð¸Ð¼Ðµ ÑÐµÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð²ÑÐµÐ¼ÐµÐ½Ð¸ Ð±ÑÐ»Ð¾ ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¾ Ð¿Ð¾Ð²ÑÑÐµÐ½Ð¸Ðµ ÑÐºÑÐ¿ÑÐµÑÑÐ¸Ð¸ Ð³ÐµÐ½Ð¾Ð², Ð¾ÑÐ²ÐµÑÐ°ÑÑÐ¸Ñ Ð·Ð° Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ñ ÑÐ¾ÑÑÐ°Ð½ÐµÐ½Ð¸Ñ ÐÐÐ, Ð°ÑÑÐ¾ÑÐ°Ð³Ð¸Ñ, ÑÐ¿Ð¸ÑÐµÐ»Ð¸Ð°Ð»ÑÐ½Ð¾-Ð¼ÐµÐ·ÐµÐ½ÑÐ¸Ð¼Ð°Ð»ÑÐ½ÑÐ¹ Ð¿ÐµÑÐµÑÐ¾Ð´, Ð¼Ð¸Ð³ÑÐ°ÑÐ¸Ñ Ð¸ Ð¿ÑÐ¾Ð»Ð¸ÑÐµÑÐ°ÑÐ¸Ñ ÐºÐ»ÐµÑÐ¾Ðº, Ð¸Ð½ÐºÑÐ±Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ Ð½Ð° Ð¼Ð°ÑÑÐ¸ÐºÑÐµ Ð¿Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ Ñ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½Ð¾Ð¹ Ð»Ð¸Ð½Ð¸ÐµÐ¹. ÐÑÐ¾Ð¼Ðµ ÑÐ¾Ð³Ð¾, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÐµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸ÑÐµÑÐºÐ¸Ñ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸Ðº Ð±ÑÐ»Ð¸ Ð¿Ð¾Ð´ÑÐ²ÐµÑÐ¶Ð´ÐµÐ½Ñ ÑÐµÑÑÐ°Ð¼Ð¸ Ð½Ð° Ð¿ÑÐ¾Ð»Ð¸ÑÐµÑÐ°ÑÐ¸Ñ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ ÐºÑÐ»ÑÑÑÑ. ÐÐµÑÐ¾Ð´Ð¾Ð¼ western-blot Ð±ÑÐ»Ð° Ð¾ÑÐµÐ½ÐµÐ½Ð° Ð°ÐºÑÐ¸Ð²Ð°ÑÐ¸Ñ Ð·Ð°ÑÐ¸ÑÐ½ÑÑ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð¾Ð² ÐºÑÐ»ÑÑÑÑÑ ÐºÐ»ÐµÑÐ¾Ðº HOS Ð¿Ð¾ÑÐ»Ðµ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ñ ÐÐÐ Ð¿Ð¾ Ð½Ð°ÐºÐ¾Ð¿Ð»ÐµÐ½Ð¸Ñ Ð±ÐµÐ»ÐºÐ¾Ð², ÑÑÐ°ÑÑÐ²ÑÑÑÐ¸Ñ Ð² ÑÐ°Ð¿ÐµÑÐ¾Ð½Ð½Ð¾Ð¹ Ð¸ Ð°ÑÑÐ¾ÑÐ°Ð³Ð¸ÑÐµÑÐºÐ¾Ð¹ ÑÐ¸ÑÑÐµÐ¼Ð°Ñ. Ð¢Ð°ÐºÐ¶Ðµ Ð±ÑÐ» Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½ ÐÐ¢Ð¢-ÑÐµÑÑ, ÑÐ²Ð¸Ð´ÐµÑÐµÐ»ÑÑÑÐ²ÑÑÑÐ¸Ð¹ Ð¾Ð± ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾ÑÑÐ¸ Ðº ÑÐ¸Ð¼Ð¸Ð¾Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ°Ð¼ ÐºÐ»ÐµÑÐ¾Ðº, Ð¸Ð½ÐºÑÐ±Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÑ Ð½Ð° Ð²Ð½ÐµÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¼ Ð¼Ð°ÑÑÐ¸ÐºÑÐµ, Ð¿Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ Ñ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½Ð¾Ð¹ Ð³ÑÑÐ¿Ð¿Ð¾Ð¹. Ð¢Ð°ÐºÐ¸Ð¼ Ð¾Ð±ÑÐ°Ð·Ð¾Ð¼, ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð¼ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÐ²Ð»ÑÐµÑÑÑ Ð¿Ð¾Ð½Ð¸Ð¼Ð°Ð½Ð¸Ðµ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ð¹ Ð²Ð½ÐµÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð³Ð¾ Ð¼Ð°ÑÑÐ¸ÐºÑÐ° Ñ ÐºÐ»ÐµÑÐºÐ°Ð¼Ð¸, Ð²Ð»Ð¸ÑÐ½Ð¸Ð¹ Ð½Ð° Ð¿ÐµÑÐµÐ´Ð°ÑÑ ÑÐ¸Ð³Ð½Ð°Ð»Ð¾Ð² Ð² ÐºÐ»ÐµÑÐºÐ¸. ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð¾Ð¶ÐµÑ Ð±ÑÑÑ Ð¿Ð¾Ð»ÐµÐ·Ð½Ð° Ð´Ð»Ñ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ¸ Ð½Ð¾Ð²ÑÑ ÑÑÑÐ°ÑÐµÐ³Ð¸Ð¹ Ð»ÐµÑÐµÐ½Ð¸Ñ Ð¾Ð½ÐºÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ð¹ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ ÐÐÐ.
description: This work is devoted to establishing the role of the extracellular matrix (ECM) as a component of the microenvironment of tumor cells on their progression. During the study, a protocol for obtaining ones own extracellular matrix from osteosarcoma cancer cells was developed and tested. Real-time PCR tests revealed an increase in the expression of genes responsible for DNA retention mechanisms, autophagy, epithelial-mesenchymal transition, migration and proliferation of cells incubated on the matrix compared to the control line. In addition, the obtained metabolic characterization results were confirmed by cell culture proliferation tests. The western blot method was used to evaluate the activation of protective mechanisms of HOS cell culture after interaction with the ECM by the accumulation of proteins involved in the chaperone and autophagic systems. An MTT test was also performed, indicating the resistance to chemotherapy of cells incubated on the extracellular matrix compared to the control group. Thus, the result of the study is an understanding of the interactions of the extracellular matrix with cells and the effects on signal transmission into cells. This work may be useful for the development of new strategies for treating cancer diseases based on ECM.