title: ÐÐ·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ðµ Ð½ÐµÐ²Ð¸ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ Ð ÐÐ-Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ñ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð° phiKZ c ÐÐÐ
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Interaction of non-virion RNA-polymerase bacteriophage phiKZ with DNA
Creators: name: ÐÐ¸ÑÐ²Ð¸Ð½Ð¾Ð²Ð°, ÐÐ½Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: ÐÐµÐ»ÐºÐ¸
Ð¤ÐµÑÐ¼ÐµÐ½ÑÑ
ÐÐ¸ÐºÑÐ¾Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸Ñ
ÐÐÐ-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð°Ñ Ð ÐÐ-Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð°
Ð³Ð¸Ð³Ð°Ð½ÑÑÐºÐ¸Ðµ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð¸
Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³ phiKZ
ÐÐÐ-Ð±ÐµÐ»ÐºÐ¾Ð²ÑÐµ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ
Ð½ÐµÐ²Ð¸ÑÐ¸Ð¾Ð½Ð½Ð°Ñ Ð ÐÐ-Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ð°
Ð¾Ð³ÑÐ°Ð½Ð¸ÑÐµÐ½Ð½ÑÐ¹ Ð¿ÑÐ¾ÑÐµÐ¾Ð»Ð¸Ð·

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð° Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ð½ÐµÐºÐ°Ð½Ð¾Ð½Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð½ÐµÐ²Ð¸ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ ÐÐÐ-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾Ð¹ Ð ÐÐ-Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ñ Ð±Ð°ÐºÑÐµÑÐ¸Ð¾ÑÐ°Ð³Ð° phiKZ. ÐÐ½Ð° ÑÐ¾ÑÑÐ¾Ð¸Ñ Ð¸Ð· 4 ÑÑÐ±ÑÐµÐ´Ð¸Ð½Ð¸Ñ, ÑÐ°ÑÑÐ¸ÑÐ½Ð¾ Ð³Ð¾Ð¼Ð¾Ð»Ð¾Ð³Ð¸ÑÐ½ÑÑ Ð´Ð²ÑÐ¼ ÐºÐ°ÑÐ°Ð»Ð¸ÑÐ¸ÑÐµÑÐºÐ¸Ð¼ ÑÑÐ±ÑÐµÐ´Ð¸Ð½Ð¸ÑÐ°Ð¼ Ð±Ð°ÐºÑÐµÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð¹ Ð ÐÐÐ, Ð¸ 5 ÑÑÐ±ÑÐµÐ´Ð¸Ð½Ð¸ÑÑ Ð±ÐµÐ· Ð³Ð¾Ð¼Ð¾Ð»Ð¾Ð³Ð¾Ð² Ñ Ð¸Ð·Ð²ÐµÑÑÐ½Ð¾Ð¹ ÑÑÐ½ÐºÑÐ¸ÐµÐ¹. Ð Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ Ð²ÐµÐ´ÐµÑÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ ÑÐ¾Ð»Ð¸ Ð¿ÑÑÐ¾Ð¹ ÑÑÐ±ÑÐµÐ´Ð¸Ð½Ð¸ÑÑ Ð² ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐµ Ð½Ð²Ð ÐÐÐ. Ð Ð°Ð½ÐµÐµ Ð±ÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ð²ÐµÑÐ¾ÑÑÐ½Ð¾ Ð¿ÑÑÐ°Ñ Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼Ð° Ð´Ð»Ñ ÑÐ±Ð¾ÑÐºÐ¸ Ð°ÐºÑÐ¸Ð²Ð½Ð¾Ð¹ Ð½Ð²Ð ÐÐÐ Ð¸ Ð¿ÑÐ¸Ð½Ð¸Ð¼Ð°ÐµÑ ÑÑÐ°ÑÑÐ¸Ðµ Ð²Ð¾ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ðµ Ñ ÐÐÐ.. ÐÐµÑÐ¾Ð´ Ð¾Ð³ÑÐ°Ð½Ð¸ÑÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¿ÑÐ¾ÑÐµÐ¾Ð»Ð¸Ð·Ð° Ð±ÑÐ» Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½ Ð´Ð»Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÐÐÐ-Ð±ÐµÐ»ÐºÐ¾Ð²ÑÑ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑÐ¾Ð² Ð½Ð²Ð ÐÐÐ Ñ ÑÐ°Ð·Ð½ÑÐ¼Ð¸ ÑÐ¸Ð¿Ð°Ð¼Ð¸ ÐÐÐ-Ð¼Ð°ÑÑÐ¸Ñ. ÐÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ ÑÐ²ÑÐ·ÑÐ²Ð°Ð½Ð¸Ðµ Ñ ÐÐÐ ÑÐ¼ÐµÐ½ÑÑÐ°ÐµÑ Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¿ÑÐ¾ÑÐµÐ°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ ÑÑÐ°Ð³Ð¼ÐµÐ½ÑÐ° Ð¿ÑÑÐ¾Ð¹ ÑÑÐ±ÑÐµÐ´Ð¸Ð½Ð¸ÑÑ, ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²ÑÑÑÐµÐ³Ð¾ 1-382 Ð°Ð°. Ð¡ ÑÐµÐ»ÑÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð½Ð° ÑÑÐ¾ ÐµÑÐµ Ð²Ð»Ð¸ÑÐµÑ Ð¡-ÐºÐ¾Ð½ÐµÑ Ð¿ÑÑÐ¾Ð¹ ÑÑÐ±ÑÐµÐ´Ð¸Ð½Ð¸ÑÑ Ð±ÑÐ»Ð¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ñ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÑ Ð¿Ð¾ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑ, Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð¸Ð· Ð½ÐµÐ³Ð¾ Ð¸ 4Ñ ÑÑÐ±ÑÐµÐ´Ð¸Ð½Ð¸ÑÐ½Ð¾Ð¹ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ°Ð·Ñ in vivo. Ð ÑÐ¾Ð´Ðµ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¾ Ð²ÑÑÑÐ½ÐµÐ½Ð¾, ÑÑÐ¾ C-ÐºÐ¾Ð½ÐµÑ Ð¿ÑÑÐ¾Ð¹ ÑÑÐ±ÑÐµÐ´Ð¸Ð½Ð¸ÑÑ Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼ Ð²Ð¾ Ð²ÑÐµÐ¼Ñ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ñ ÐÐÐ-Ð¼Ð°ÑÑÐ¸ÑÐ°Ð¼Ð¸ Ð¸ Ð¾ÑÐ¾Ð±ÐµÐ½Ð½Ð¾ Ð¿ÑÐ¸ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ðµ Ñ Â«Ð´Ð»Ð¸Ð½Ð½ÑÐ¼Ð¸Â» ÐÐÐ Ð¼Ð°ÑÑÐ¸ÑÐ°Ð¼Ð¸, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¾Ð½ Ð²Ð°Ð¶ÐµÐ½ Ð½Ð° ÑÑÐ°Ð¿Ðµ ÑÐ±Ð¾ÑÐºÐ¸ ÑÐµÑÐ¼ÐµÐ½ÑÐ° in vivo.â
description: The given work is devoted to study of the mechanism of action of the non-canonical non-virion DNA-dependent RNA-polymerase encoded by giant bacterio-phage phiKZ. It consists of 4 subunits which are homologs of two catalytic subunits of bacterial RNAP, and 5th subunit which have not homologs with a known function. The role of the fifth subunit in the nvRNKP complex is investigated at the laboratory. It was shown that the fifth is necessary for the assembly of active nvPNKP and takes part in interaction with DNA. The limited proteolysis method was used to investigate DNA-protein complex-es of nvPNAP with different types of DNA-matrices. Binding with DNA reduce the formation of a protealized fragment of the fifth subunit corresponding to 1-382 aa. In order to study what else is affected by the C-terminal of the fifth subunit, experiments were carried out to emit the complex formed from it and the 4x subunit polymerase in vivo. It was determined that the C-end of the fifth subunit is necessary during interac-tion with DNA and especially during interacting with "long" DNA, and it is also im-portant for the assembly of enzyme in vivo.