title: ÐÐ·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²Ð¸Ðµ Ñ EB-Ð±ÐµÐ»ÐºÐ°Ð¼Ð¸ ÑÐµÐ³ÑÐ»Ð¸ÑÑÐµÑ ÐºÐ»Ð°ÑÑÐµÑÐ¸Ð·Ð°ÑÐ¸Ñ Ð±ÐµÐ»ÐºÐ¾Ð² STIM
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Interaction with EB proteins controls STIM2 protein clustering
Creators: name: Ð Ð°ÐºÐ¾Ð²ÑÐºÐ°Ñ, ÐÐ½Ð°ÑÑÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÐÐµÐ»ÐºÐ¸
Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÐ¹ ÑÐ¸Ð¿Ð¸Ðº
ÑÐ½Ð´Ð¾Ð¿Ð»Ð°Ð·Ð¼Ð°ÑÐ¸ÑÐµÑÐºÐ¸Ð¹ ÑÐµÑÐ¸ÐºÑÐ»ÑÐ¼
Ð´ÐµÐ¿Ð¾-ÑÐ¿ÑÐ°Ð²Ð»ÑÐµÐ¼ÑÐ¹ Ð²Ñ Ð¾Ð´ ÐºÐ°Ð»ÑÑÐ¸Ñ
Ð´Ð¸Ð½Ð°Ð¼Ð¸ÑÐµÑÐºÐ¸Ðµ Ð¼Ð¸ÐºÑÐ¾ÑÑÑÐ±Ð¾ÑÐºÐ¸
dendritic spines
endoplasmic reticulum
store operated calcium entry
dynamic microtubules

description: ÐÐ´Ð½Ð¸Ð¼ Ð¸Ð· Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð¾Ð² ÐºÐ°Ð»ÑÑÐ¸ÐµÐ²Ð¾Ð³Ð¾ ÑÐ¸Ð³Ð½Ð°Ð»Ð¸Ð½Ð³Ð° Ð² Ð½ÐµÐ¹ÑÐ¾Ð½Ðµ ÑÐ²Ð»ÑÐµÑÑÑ Ð´ÐµÐ¿Ð¾-ÑÐ¿ÑÐ°Ð²Ð»ÑÐµÐ¼ÑÐ¹ Ð²ÑÐ¾Ð´ ÐºÐ°Ð»ÑÑÐ¸Ñ (ÐÐ£ÐÐ). ÐÐ£ÐÐ Ð¸Ð· Ð²Ð½ÐµÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ ÑÑÐµÐ´Ñ Ð² ÑÐ¸ÑÐ¾Ð¿Ð»Ð°Ð·Ð¼Ñ Ð°ÐºÑÐ¸Ð²Ð¸ÑÑÐµÑÑÑ Ð¿ÑÐ¸ ÑÐ½Ð¸Ð¶ÐµÐ½Ð¸Ð¸ ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸Ð¸ ÐºÐ°Ð»ÑÑÐ¸Ñ Ð²Ð¾ Ð²Ð½ÑÑÑÐ¸ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐ¼ ÐºÐ°Ð»ÑÑÐ¸ÐµÐ²Ð¾Ð¼ Ð´ÐµÐ¿Ð¾ â Ð³Ð»Ð°Ð´ÐºÐ¾Ð¼ ÑÐ½Ð´Ð¾Ð¿Ð»Ð°Ð·Ð¼Ð°ÑÐ¸ÑÐµÑÐºÐ¾Ð¼ ÑÐµÑÐ¸ÐºÑÐ»ÑÐ¼Ðµ (Ð­Ð ). Ð Ð°ÑÐ¿Ð¾Ð»Ð°Ð³Ð°ÑÑÐ¸Ð¹ÑÑ Ð½Ð° Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ðµ ÑÐ½Ð´Ð¾Ð¿Ð»Ð°Ð·Ð¼Ð°ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐµÑÐ¸ÐºÑÐ»ÑÐ¼Ð° Ð±ÐµÐ»Ð¾Ðº-ÐºÐ°Ð»ÑÑÐ¸ÐµÐ²ÑÐ¹ ÑÐµÐ½ÑÐ¾Ñ STIM Ð¿ÑÐ¸ Ð¾Ð¿ÑÑÑÐ¾ÑÐµÐ½Ð¸Ð¸ ÐºÐ°Ð»ÑÑÐ¸ÐµÐ²Ð¾Ð³Ð¾ Ð´ÐµÐ¿Ð¾, ÑÑÐ°Ð½ÑÐ»Ð¾ÑÐ¸ÑÑÐµÑÑÑ Ðº ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ Ð¼ÐµÐ¼Ð±ÑÐ°Ð½Ðµ, Ð³Ð´Ðµ ÑÐ¾ÑÐ¼Ð¸ÑÑÐµÑ ÐºÐ»Ð°ÑÑÐµÑÑ Ð¸ Ð·Ð°Ð¿ÑÑÐºÐ°ÐµÑ Ð²ÑÐ¾Ð´ ÐºÐ°Ð»ÑÑÐ¸Ñ Ð² Ð½ÐµÐ¹ÑÐ¾Ð½. ÐÐ°ÑÑÑÐµÐ½Ð¸Ðµ Ð´ÐµÐ¿Ð¾-ÑÐ¿ÑÐ°Ð²Ð»ÑÐµÐ¼Ð¾Ð³Ð¾ Ð²ÑÐ¾Ð´Ð° ÐºÐ°Ð»ÑÑÐ¸Ñ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¾ Ð¿ÑÐ¸ Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ ÐÐ»ÑÑÐ³ÐµÐ¹Ð¼ÐµÑÐ°. Ð ÑÐ°Ð±Ð¾ÑÐµ ÑÐ°ÑÑÐ¼Ð¾ÑÑÐµÐ½Ð¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ ÑÑÐ±ÑÐ»Ð¸Ð½Ð¾Ð²ÑÑ Ð¼Ð¸ÐºÑÐ¾ÑÑÑÐ±Ð¾ÑÐµÐº Ð½Ð° ÐºÐ»Ð°ÑÑÐµÑÐ¸Ð·Ð°ÑÐ¸Ñ Ð±ÐµÐ»ÐºÐ¾Ð² STIM1/2 Ð¸ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ñ Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÑ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð² Ð² Ð½Ð¾ÑÐ¼Ðµ Ð¸ Ð½Ð° ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð±Ð¾Ð»ÐµÐ·Ð½Ð¸ ÐÐ»ÑÑÐ³ÐµÐ¹Ð¼ÐµÑÐ°. ÐÑÐ» Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½ ÑÐ¿Ð»Ð°Ð¹Ñ Ð²Ð°ÑÐ¸Ð°Ð½Ñ Ð±ÐµÐ»ÐºÐ° STIM1 â Bv1-STIM1, Ð¸Ð·Ð²ÐµÑÑÐ½Ð¾, ÑÑÐ¾ Ð¾Ð½ Ð½Ðµ Ð²Ð·Ð°Ð¸Ð¼Ð¾Ð´ÐµÐ¹ÑÑÐ²ÑÐµÑ Ñ Orai1, Ð¾Ð´Ð½Ð°ÐºÐ¾ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð½Ð° Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÐµ ÑÐ¸Ð¿Ð¸ÐºÐ¸ ÑÐ°ÑÑÐ¼Ð¾ÑÑÐµÐ½Ð¾ Ð½Ðµ Ð±ÑÐ»Ð¾. ÐÐ¸Ð¿ÐµÑÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Ð±ÐµÐ»ÐºÐ° STIM1 Ð²ÑÐ·ÑÐ²Ð°ÐµÑ ÑÐ²ÐµÐ»Ð¸ÑÐµÐ½Ð¸Ðµ Ð´Ð»Ð¸Ð½Ñ ÑÐµÐ¹ÐºÐ¸ Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÑ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð² Ð² Ð½Ð¾ÑÐ¼Ðµ. ÐÐ¸Ð¿ÐµÑÑÐºÑÐ¿ÑÐµÑÑÐ¸Ñ Bv1-STIM1 ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð° ÑÐ²ÐµÐ»Ð¸ÑÐ¸Ð²Ð°ÑÑ Ð¿Ð»Ð¾ÑÐ°Ð´Ñ Ð³Ð¾Ð»Ð¾Ð²ÐºÐ¸ Ñ Ð´ÐµÐ½Ð´ÑÐ¸ÑÐ½ÑÑ ÑÐ¸Ð¿Ð¸ÐºÐ¾Ð² Ð² Ð½ÐµÐ¹ÑÐ¾Ð½Ð°Ñ PS1-M146V, Ð¼Ð¾Ð´ÐµÐ»Ð¸ÑÑÑÑÐ¸Ñ ÐÐ. ÐÐ²ÑÐ¾ÑÐ¾Ð¼ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½ Ð¸ ÑÑÐ¿ÐµÑÐ½Ð¾ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½ Ð¼ÐµÑÐ¾Ð´ Ð²Ð¸Ð·ÑÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ð¸ Ð¸ Ð°Ð½Ð°Ð»Ð¸Ð·Ð° Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÐ¸ Ð¸ ÑÐ°Ð·Ð¼ÐµÑÐ¾Ð² ÐºÐ»Ð°ÑÑÐµÑÐ¾Ð² Ð±ÐµÐ»ÐºÐ¾Ð² STIM1/2 Ð¿ÑÐ¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ð¸ ÑÐºÑÐ¿Ð°Ð½ÑÐ¸Ð¾Ð½Ð½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸ Ð½Ð° ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ Ð»Ð¸Ð½Ð¸Ð¸ HEK293T. Ð¡ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸ÐµÐ¼ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð½Ð¾Ð³Ð¾ Ð¼ÐµÑÐ¾Ð´Ð° Ð½Ð° ÐºÐ»ÐµÑÐ¾ÑÐ½Ð¾Ð¹ Ð»Ð¸Ð½Ð¸Ð¸ HEK293T Ð¿ÑÐ¾Ð´ÐµÐ¼Ð¾Ð½ÑÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¾, ÑÐ°Ð·ÑÑÐ² ÑÐ²ÑÐ·Ð¸ Ñ ÐÐ ÑÑÐ¸Ð¼ÑÐ»Ð¸ÑÑÐµÑÑÑ Ð¾Ð±ÑÐ°Ð·Ð¾Ð²Ð°Ð½Ð¸Ðµ ÐºÐ»Ð°ÑÑÐµÑÐ¾Ð² Ð±ÐµÐ»ÐºÐ° STIM1, Ð° Ð´Ð»Ñ Ð±ÐµÐ»ÐºÐ° STIM2 Ð½Ðµ Ð±ÑÐ»Ð¾ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¾ ÑÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÐ°Ð·Ð»Ð¸ÑÐ¸Ð¹ Ð¿Ð¾ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÐ°Ð¼: Ð¿Ð»Ð¾ÑÐ½Ð¾ÑÑÐ¸ Ð¸ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²Ñ ÐºÐ»Ð°ÑÑÐµÑÐ¾Ð² ÐºÐ°Ðº Ð² Ð¾Ð±ÑÑÐ½ÑÑ ÑÑÐ»Ð¾Ð²Ð¸ÑÑ, ÑÐ°Ðº Ð¸ Ð±ÐµÐ· ÐºÐ°Ð»ÑÑÐ¸Ñ.
description: One of the calcium signaling mechanisms in a neuron is the store operated calcium entry (SOCE). SOCE is activated from the extracellular environment to the cytoplasm when the calcium concentration in the intracellular calcium depot, the smooth (ER), decreases. The protein-calcium sensor STIM, which is located on the membrane of the endoplasmic reticulum, when the calcium depot is empty, translocates to the cell membrane, where it forms clusters and triggers calcium to enter the neuron. The depot-controlled calcium intake disturbance was found in Alzheimer's disease. The study shows the influence of tubulin microtubules in the clustering of STIM proteins and the morphology of dendritic spines in normal conditions and in the cell model of Alzheimer's disease. Overexpression of the STIM1 protein causes an increase in the neck length of dendritic spines in normal conditions. Overexpression of Bv1-STIM1 is able to increase the head area of dendritic spines in PS1-M146V neurons. The author has developed and successfully applied the visualization method, density and size analysis of STIM1/2 protein clusters using expansion microscopy on the HEK293T cell line. It was demonstrated, using the developed method on the HEK293T cell line, that interaction with microtubules, connected with EB proteins, is an obstacle to the formation of STIM1 protein clusters. The data obtained for the first time demonstrated the opposite role of EB proteins in the clustering of STIM1 and STIM2 homologues, and also showed the neuroprotective effect of tubulin microtubules on the dendritic spines morphology.