cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Local T/B cooperation in inflamed tissues is supported by T follicular helper-like cells
Creators: name: Van, Dana Vu
name: Beier, Katja C.
name: Pietzke, Lea-Jean
name: Baz, Maysun S. Al
name: Feist, Randi K.
name: Gurka, Stephanie
name: Hamelmann, Eckard
name: Kroczek, Richard
name: Hutloff, Andreas
Publisher: name: Robert Koch-Institut
Year: 2016
Subjects: subject: Animals
subject: Mice
subject: Enzyme-Linked Immunosorbent Assay
subject: Disease Models Animal
subject: Mice Transgenic
subject: Lymphoid Tissue/immunology
subject: Antibody Formation/immunology
subject: Lung/immunology
subject: B-Lymphocytes/immunology
subject: Flow Cytometry
subject: Autoimmunity/immunology
subject: Coculture Techniques
subject: Germinal Center/immunology
subject: Inflammation
subject: Lymph Nodes/immunology
subject: Lymphocyte Cooperation/immunology
subject: Ovalbumin/toxicity
subject: Pneumonia/chemically induced
subject: Pneumonia/immunology
subject: Proto-Oncogene Proteins c-bcl-6
subject: Receptors Antigen
subject: T-Cell/genetics
subject: Receptors CXCR5
subject: T-Lymphocyte Subsets/immunology
subject: T-Lymphocytes Helper-Inducer/immunology
subject: 610 Medizin

description: Autoimmune diseases and other inflammatory conditions are characterized by large lymphocytic tissue infiltrates in which T and B cells can be found in close contact. Here, using a murine airway inflammation model, we compare antigen-specific T and B cells in lung tissue versus lung-draining lymph node. In the lung we identify a B-cell population exhibiting a classical germinal centre phenotype without being organized into ectopic lymphoid tissue. By contrast, classical CXCR5+ Bcl-6+ T follicular helper cells are not present. Nevertheless, lung-infiltrating T cells exhibit follicular helper-like properties including the potential to provide help to naive B cells. The lung tissue is also a survival niche for memory T and B cells remaining in residual peribronchial infiltrates after resolution of inflammation. Collectively, this study shows the importance of T/B cooperation not only in lymph nodes but also in inflamed peripheral tissues for local antibody responses to infection and autoimmunity.
sj-docx-1-mcr-10.1177_10775587211028068 – Supplemental material for How Can Implementation of a Large-Scale Patient Safety Program Strengthen Hospital Safety Culture? Lessons From a Qualitative Study of National Patient Safety Program Implementation in Two Public Hospitals in Brazil
Creators: name: Caldas, Bárbara do Nascimento
name: Portela, Margareth Crisóstomo
name: Singer, Sara Jean
name: Aveling, Emma-Louise
Publisher: name: SAGE Journals
Year: 2021
Subjects: subject: 111799 Public Health and Health Services not elsewhere classified
subject: FOS: Health sciences
subject: 160807 Sociological Methodology and Research Methods
subject: FOS: Sociology

description: Supplemental material, sj-docx-1-mcr-10.1177_10775587211028068 for How Can Implementation of a Large-Scale Patient Safety Program Strengthen Hospital Safety Culture? Lessons From a Qualitative Study of National Patient Safety Program Implementation in Two Public Hospitals in Brazil by Bárbara do Nascimento Caldas, Margareth Crisóstomo Portela, Sara Jean Singer and Emma-Louise Aveling in Medical Care Research and Review
Ion Mobility Spectrometry: Optimization of Parameters in Collision Cross Sections and Trace Detection of Explosives
Creators: name: Wu, Tianyang
Publisher: name: Purdue University Graduate School
Year: 2019
Subjects: Mechanical engineering not elsewhere classified

description: Ion mobility spectrometry is a powerful technique for the study related to molecule. The work of tow major applications are introduced in this paper. The first application is the optimization of parameters in CCS. The accurate calculation of the collision cross section for multiple molecules is a long-time interested topic in the research for substances detection in micro scale. No reliable analytical approach to calculate the collision cross section has been established to date. Different approaches rely on different mechanism will provide different results in significant extent. This work introduce a method for the determination of parameters in the Lennard Jones potential. Experimental data combined with numerical computation was the fundamental strategy during the optimization of the parameters. In the experiment, electrospray is used as the ion source of IMS while a nebulizer was utilized to electrify the aromatic compounds. New parameters show no less accuracy and equal efficiency while can explain the physical meaning of the collision more clearly. The second application is the trace detection of explosives with very low concentration. The detection of explosives is an important topic in security, while the detection will be difficult due to the low vapor pressure of explosives. In this work, two types of devices are designed for the trace detection of explosives at an extremely low concentration. TNT is selected as the explosives in the experiment. The experiment succeed to reach a sensitivity of 1 part per quintillion, and even find out a linear relationship between the logarithm of TNT concentration and TNT vapor pressure.
title: Sputter deposition and plasma modification of tungsten alloys for nuclear fusion applications
Creators: name: Mahoney, Nicholas
Publisher: name: The Australian National University
Year: 2025
Subjects: subject: Nuclear Fusion
subject: ITER

description: The ITER reactor aims to be first thermonuclear fusion device to demonstrate net fusion power. Critical plasma-facing components in the reactor will be made of tungsten, but helium plasma irradiation is known to embrittle tungsten metal. A future reactor will therefore need an alternative material to extend the lifetime of the components. This thesis investigated the use of tungsten alloy films as a plasma-facing material for a future reactor. Tantalum and chromium were chosen as the alloying elements, and 60 − 100 nm films were manufactured using magnetron sputter deposition at a variety of alloy concentrations. Tungsten alloy films are known to deposit in an undesirable A15 crystal phase. The films were heat treated at 650◦C for 1 hour, 2 hours and 4 hours to induce and investigate the phase transition from A15 to BCC structure. X-ray diffraction patterns confirmed the presence of an A15 structure prior to annealing. The A15 phase was very stable in comparison with past results, requiring over 2 hours at 650◦C to transition to BCC. This was speculated to be linked to oxygen trapped in the films during deposition. The phase transition was slower in alloyed films than a pure tungsten control, with the retarding effect of tantalum stronger than chromium, which suggested the alloying atoms further stabilised the deposited A15 structure. Resistivity of the films saw a reduction with annealing time characteristic of the phase transition. The annealed alloy films were then exposed to helium plasma in the Magnetised Plasma Interaction Experiment at 300◦C, 500◦C and 800◦C. Surface morphology changes were tracked with secondary electron imaging. At 800◦C, surface pits were seen, which were larger in a pure tungsten film than in low concentration alloy films. Advanced surface roughening was observed in the chromium films, while a high concentration tantalum film had no surface damage at all. X-ray diffraction patterns confirmed these trends, and showed an increase in microstrain with the surface roughening in the chromium alloys. These results suggest W-Ta alloy films may have superior irradiation resistance than W-Cr alloy films, but more work is needed to confirm the trends in alloy concentration.
Additional file 1 of Development of rice bran-derived nanoparticles with excellent anti-cancer activity and their application for peritoneal dissemination
Creators: name: Sasaki, Daisuke
name: Suzuki, Hinako
name: Kusamori, Kosuke
name: Itakura, Shoko
name: Todo, Hiroaki
name: Nishikawa, Makiya
Publisher: name: figshare
Year: 2024
Subjects: subject: Biochemistry
subject: Space Science
subject: Medicine
subject: Cell Biology
subject: Pharmacology
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: FOS: Biological sciences
subject: Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Cancer

description: Supplementary Material 1
Externalizing Disorders: Genetics or Prenatal Alcohol Exposure?
Creators: name: Wetherill, Leah
Publisher: name: Purdue University Graduate School
Year: 2019
Subjects: Psychophysiology

description: Externalizing disorders such as attention deficit hyperactivity disorder (ADHD), conduct disorder (CD), and oppositional defiant disorder (ODD) have a high prevalence rate in both children of alcoholics and in those with prenatal alcohol exposure (PAE). These disorders are also predictors of alcohol dependence (alcdep), heritable, and share an underlying genetic liability with alcdep. Furthermore, a mother who drinks while pregnant is likely to be alcohol dependent (AD), and vice-versa. This study incorporated these factors into one model, including as well as a measure of broad genetic risk for ADHD and alcdep to test for the contributions of these effects simultaneously. An independent sample was used to confirm the results for PAE and broad genetic risk. The hypothesis is that PAE will increase the risk to ADHD but not to CD or ODD.
Occupational Injuries in Germany: Population-Wide National Survey Data Emphasize the Importance of Work-Related Factors
Creators: name: Rommel, Alexander
name: Varnaccia, Gianni
name: Lahmann, Nils
name: Kottner, Jan
name: Kroll, Lars Eric
Publisher: name: Robert Koch-Institut, Epidemiologie und Gesundheitsberichterstattung
Year: 2016
Subjects: subject: 610 Medizin

description: Unintentional injuries cause much of the global mortality burden, with the workplace being a common accident setting. Even in high-income economies, occupational injury figures remain remarkably high. Because risk factors for occupational injuries are prone to confounding, the present research takes a comprehensive approach. To better understand the occurrence of occupational injuries, sociodemographic factors and work- and health-related factors are tested simultaneously. Thus, the present analysis aims to develop a comprehensive epidemiological model that facilitates the explanation of varying injury rates in the workplace. The representative phone survey German Health Update 2010 provides information on medically treated occupational injuries sustained in the year prior to the interview. Data were collected on sociodemographics, occupation, working conditions, health-related behaviors, and chronic diseases. For the economically active population (18–70 years, n = 14,041), the 12-month prevalence of occupational injuries was calculated with a 95% confidence interval (CI). Blockwise multiple logistic regression was applied to successively include different groups of variables. Overall, 2.8% (95% CI 2.4–3.2) of the gainfully employed population report at least one occupational injury (women: 0.9%; 95% CI 0.7–1.2; men: 4.3%; 95% CI 3.7–5.0). In the fully adjusted model, male gender (OR 3.16) and age 18–29 (OR 1.54), as well as agricultural (OR 5.40), technical (OR 3.41), skilled service (OR 4.24) or manual (OR 5.12), and unskilled service (OR 3.13) or manual (OR 4.97) occupations are associated with higher chances of occupational injuries. The same holds for frequent stressors such as heavy carrying (OR 1.78), working in awkward postures (OR 1.46), environmental stress (OR 1.48), and working under pressure (OR 1.41). Among health-related variables, physical inactivity (OR 1.47) and obesity (OR 1.73) present a significantly higher chance of occupational injuries. While the odds for most work-related factors were as expected, the associations for health-related factors such as smoking, drinking, and chronic diseases were rather weak. In part, this may be due to context-specific factors such as safety and workplace regulations in high-income countries like Germany. This assumption could guide further research, taking a multi-level approach to international comparisons.
sj-pdf-1-sgo-10.1177_21582440211032162 – Supplemental material for What is Known About Students and Sleep: Systematic Review and Evidence Map
Creators: name: Bjørnnes, Ann Kristin
name: Torbjørnsen, Astrid
name: Valeberg, Berit Taraldsen
name: Sparboe-Nilsen, B. Bente
name: Sandbekken, Ida Hellum
name: Almendingen, Kari
name: Leegaard, Marit
name: Ravn, Ingrid
name: Sæterstrand, M. Torill
name: Løyland, Borghild
name: Kvarme, Lisbeth Gravdal
name: Fagerlund, Bettina Holmberg
name: Valla, Lisbeth
name: Misvær, Nina
name: Riiser, Kirsti
name: Utne, Inger
name: Rostad, Hanne
name: Winger, Anette
name: Albertini Früh, Elena
name: Holmen, Heidi
name: Lis, Ribu
name: Helseth, Sølvi
name: Grov, Ellen Karine
Publisher: name: SAGE Journals
Year: 2021
Subjects: subject: 200199 Communication and Media Studies not elsewhere classified
subject: FOS: Media and communications
subject: 160299 Criminology not elsewhere classified
subject: FOS: Law
subject: Education
subject: 170199 Psychology not elsewhere classified
subject: FOS: Psychology
subject: Sociology
subject: FOS: Sociology

description: Supplemental material, sj-pdf-1-sgo-10.1177_21582440211032162 for What is Known About Students and Sleep: Systematic Review and Evidence Map by Ann Kristin Bjørnnes, Astrid Torbjørnsen, Berit Taraldsen Valeberg, B. Bente Sparboe-Nilsen, Ida Hellum Sandbekken, Kari Almendingen, Marit Leegaard, Ingrid Ravn, M. Torill Sæterstrand, Borghild Løyland, Lisbeth Gravdal Kvarme, Bettina Holmberg Fagerlund, Lisbeth Valla, Nina Misvær, Kirsti Riiser, Inger Utne, Hanne Rostad, Anette Winger, Elena Albertini Früh, Heidi Holmen, Ribu Lis, Sølvi Helseth and Ellen Karine Grov in SAGE Open
Distribution of Populations and Suitable Habitat for Spotted Turtles (Clemmys guttata) and Blanding’s Turtles (Emydoidea blandingii) in Indiana
Creators: name: Hinson, Jessica
Publisher: name: Purdue University Graduate School
Year: 2019
Subjects: Conservation and biodiversity
Wildlife and habitat management

description: The spotted turtle (Clemmys guttata) and Blanding’s turtle (Emydoidea blandingii) are two state-endangered species in Indiana whose populations are in decline. Historically, both species were found across the northern portion of Indiana in various wetland habitats. There are multiple causes of population decline for both species, including habitat fragmentation, habitat loss and degradation, urban development and encroachment, poaching, and road mortality. Despite efforts to record these species across the state, there has been no intensive population assessments. Based on this need, I conducted both visual encounter surveys across the state and used Maximum Entropy (MaxEnt) modeling to facilitate understanding the current distribution of both species in Indiana. Twenty-three locations were visited and surveyed in Indiana, with trapping being conducted at an additional four locations where populations were known to be larger. Surveys aided in delineation of six populations of Blanding’s turtles and five populations of spotted turtles. A total of 69 Blanding’s turtles and 70 spotted turtles were observed between surveying and trapping. Delineated populations were mainly found in the northern third of Indiana. This data and other occurrences were used to predict suitable habitat across Indiana. The Blanding’s turtle models were sufficiently resolved to predict potential localities or potential sites for focused management or repatriation. Spotted turtle model performance reflected the need for more samples, but also the likelihood of fewer numbers due to declining habitat availability. Both Blanding’s turtle and spotted turtle models argue for the need of more intense survey efforts based on historical occurrences, as well as restoration efforts across the state. Most models for both species were observed to have a trend towards suitable habitat in the northern third of the state, correlating with the results of the survey efforts. The results of this project indicate that Blanding’s turtle and spotted turtle populations are still in decline likely due to limited habitat availability.
Population structure and acquisition of the vanB resistance determinant in German clinical isolates of Enterococcus faecium ST192
Creators: name: Bender, Jennifer K.
name: Kalmbach, Alexander
name: Fleige, Carola
name: Klare, Ingo
name: Fuchs, Stephan
name: Werner, Guido
Publisher: name: Robert Koch-Institut, Infektionskrankheiten / Erreger
Year: 2016
Subjects: subject: 610 Medizin

description: In the context of the global action plan to reduce the dissemination of antibiotic resistances it is of utmost importance to understand the population structure of resistant endemic bacterial lineages and to elucidate how bacteria acquire certain resistance determinants. Vancomycin resistant enterococci represent one such example of a prominent nosocomial pathogen on which nation-wide population analyses on prevalent lineages are scarce and data on how the bacteria acquire resistance, especially of the vanB genotype, are still under debate. With respect to Germany, an increased prevalence of VRE was noted in recent years. Here, invasive infections caused by sequence type ST192 VRE are often associated with the vanB-type resistance determinant. Hence, we analyzed 49 vanB-positive and vanB-negative E. faecium isolates by means of whole genome sequencing. Our studies revealed a distinct population structure and that spread of the Tn1549-vanB-type resistance involves exchange of large chromosomal fragments between vanB-positive and vanB-negative enterococci rather than independent acquisition events. In vitro filter-mating experiments support the hypothesis and suggest the presence of certain target sequences as a limiting factor for dissemination of the vanB element. Thus, the present study provides a better understanding of how enterococci emerge into successful multidrug-resistant nosocomial pathogens.
BRAIN CONNECTOME NETWORK PROPERTIES VISUALIZATION
Creators: name: Zhang, Chenfeng
Publisher: name: Purdue University Graduate School
Year: 2019
Subjects: Software engineering not elsewhere classified

description: Brain connectome network visualization could help the neurologists inspect the brain structure easily and quickly. In the thesis, the model of the brain connectome network is visualized in both three dimensions (3D) environment and two dimensions (2D) environment. One is named “Brain Explorer for Connectomic Analysis” (BECA) developed by the previous research already. It could present the 3D model of brain structure with region of interests (ROIs) in different colors [5]. The other is mainly for the information visualization of brain connectome in 2D. It adopts the force-directed layout to visualize the network. However, the brain network visualization could not bring the user intuitively ideas about brain structure. Sometimes, with the increasing scales of ROIs (nodes), the visualization would bring more visual clutter for readers [3]. So, brain connectome network properties visualization becomes a useful complement to brain network visualization. For a better understanding of the effect of Alzheimer’s disease on the brain nerves, the thesis introduces several methods about the brain graph properties visualization. There are the five selected graph properties discussed in the thesis. The degree and closeness are node properties. The shortest path, maximum flow, and clique are edge properties. Except for clique, the other properties are visualized in both 3D and 2D. The clique is visualized only in 2D. For the clique, a new hypergraph visualization method is proposed with three different algorithms. Instead of using an extra node to present a clique, the thesis uses a “belt” to connect all nodes within the same clique. The methods of node connections are based on the traveling salesman problem (TSP) and Law of cosines. In addition, the thesis also applies the result of the clique to adjust the force-directed layout of brain graph in 2D to dramatically eliminate the visual clutter. Therefore, with the support of the graph properties visualization, the brain connectome network visualization tools become more flexible.
A Staphylococcus aureus Proteome Overview: Shared and Specific Proteins and Protein Complexes from Representative Strains of All Three Clades
Creators: name: Liang, Chunguang
name: Schaack, Dominik
name: Srivastava, Mugdha
name: Gupta, Shishir K.
name: Sarukhanyan, Edita
name: Giese, Anne
name: Pagels, Martin
name: Romanov, Natalie
name: Pané-Farré, Jan
name: Fuchs, Stephan
name: Dandekar, Thomas
Publisher: name: Robert Koch-Institut, Infektionskrankheiten / Erreger
Year: 2016
Subjects: subject: Staphylococcus aureus
subject: proteome
subject: protein complexes
subject: model strain
subject: 610 Medizin

description: Staphylococcus aureus is an important model organism and pathogen. This S. aureus proteome overview details shared and specific proteins and selected virulence-relevant protein complexes from representative strains of all three major clades. To determine the strain distribution and major clades we used a refined strain comparison combining ribosomal RNA, MLST markers, and looking at highly-conserved regions shared between strains. This analysis shows three sub-clades (A–C) for S. aureus. As calculations are complex and strain annotation is quite time consuming we compare here key representatives of each clade with each other: model strains COL, USA300, Newman, and HG001 (clade A), model strain N315 and Mu50 (clade B) and ED133 and MRSA252 (clade C). We look at these individual proteomes and compare them to a background of 64 S. aureus strains. There are overall 13,284 S. aureus proteins not part of the core proteome which are involved in different strain-specific or more general complexes requiring detailed annotation and new experimental data to be accurately delineated. By comparison of the eight representative strains, we identify strain-specific proteins (e.g., 18 in COL, 105 in N315 and 44 in Newman) that characterize each strain and analyze pathogenicity islands if they contain such strain-specific proteins. We identify strain-specific protein repertoires involved in virulence, in cell wall metabolism, and phosphorylation. Finally we compare and analyze protein complexes conserved and well-characterized among S. aureus (a total of 103 complexes), as well as predict and analyze several individual protein complexes, including structure modeling in the three clades.
"Global Studies." The Arden Research Handbook of Contemporary Shakespeare Criticism, ed. Evelyn Gajowski (London: Bloomsbury, 2021), pp. 247-261
Creators: name: Joubin, Alexa Alice
Publisher: name: unknown
Year: 2020
Subjects: subject: Shakespeare, William, 1564-1616
subject: Translating and interpreting
subject: Globalization
subject: Race
subject: Feminism
subject: Censorship
subject: Literature--Adaptations

description: Global studies enable us to examine deceivingly harmonious images of Shakespeare. This chapter focuses on the modern period and introduces readers to a number of key concepts in Shakespeare and global studies, namely censorship and redaction, genre, gender, race, and politics of reception. Performing Shakespeare not only creates channels between geographic spaces but also connects different time periods. Therefore, in modern times, global Shakespeares have been recruited as a transhistorical and intercultural practice to revitalize performance genres, exemplified or resisted as a colonial appendage or rhetoric and admired as a centrepiece in an exotic display.
Waning immunity against mumps in vaccinated young adults, France 2013
Creators: name: Vygen, Sabine
name: Fischer, A.
name: Meurice, L.
name: Njoya, I. Mounchetrou
name: Gregoris, M.
name: Ndiaye, B.
name: Ghenassia, A.
name: Poujol, I.
name: Stahl, J. P.
name: Antona, D.
name: Strat, Y. Le
name: Levy-Bruhl, Daniel
name: Rolland, P.
Publisher: name: Robert Koch-Institut, Infektionsepidemiologie
Year: 2016
Subjects: subject: MMR
subject: mumps
subject: third dose
subject: waning immunity
subject: 610 Medizin

description: In 2013, 15 clusters of mumps were notified in France; 72% (82/114) of the cases had been vaccinated twice with measles-mumps-rubella vaccine. To determine whether the risk of mumps increased with time since the last vaccination, we conducted a case–control study among clusters in universities and military barracks. A confirmed case had an inflammation of a salivary gland plus laboratory confirmation in 2013. A probable case presented with inflammation of a salivary gland in 2013 either lasting for > 2 days or with epidemiological link to a confirmed case. Controls had no mumps symptoms and attended the same university course, student party or military barracks. We collected clinical and vaccination data via web questionnaire and medical records. We calculated adjusted odds ratios (aOR) using logistic regression. 59% (50/85) of cases and 62% (199/321) of controls had been vaccinated twice. The odds of mumps increased for twice-vaccinated individuals by 10% for every year that had passed since the second dose (aOR 1.10; 95% confidence interval (CI): 1.02–1.19; p = 0.02). Mumps immunity waned with increasing time since vaccination. Our findings contributed to the French High Council of Public Health’s decision to recommend a third MMR dose during outbreaks for individuals whose second dose dates > 10 years.
Health and health risk behaviour of adolescents—Differences according to family structure. Results of the German KiGGS cohort study
Creators: name: Rattay, Petra
name: Lippe, Elena Von Der
name: Mauz, Elvira
name: Richter, Felicitas
name: Hölling, Heike
name: Lange, Cornelia
name: Lampert, Thomas
Publisher: name: Robert Koch-Institut, Epidemiologie und Gesundheitsberichterstattung
Year: 2018
Subjects: subject: 610 Medizin

description: Objective: This study’s aim was to investigate the association between family structure and different health-related outcomes in adolescence (self-rated health, emotional and behavioural problems, health-related quality of life, regular smoking, and heavy episodic drinking). Furthermore, we analysed the extent to which socio-economic status, family cohesion and the pre-transition health status explain family structure-related health disparities. Methods: We used longitudinal data from the first two waves of the German KiGGS cohort study carried out by the Robert Koch Institute (baseline: 2003–2006, follow-up: 2009–2012). The sample comprised 4,692 respondents aged 11 to 17 years. Using data from both waves, effects of family structure on health status at follow-up were calculated applying linear and logistic regression models. Results: We found that adolescents continuously living with both birth parents were in good health. Adolescents whose parents separated after the baseline survey, reported poorer health and were more likely to smoke. The transition from stepfamily to single parent family was also associated with a higher risk of regular smoking. Lower health-related quality of life as well as higher scores for emotional and behavioural problems occurred in almost all non-nuclear family structures, although not all effects were statistically significant. No significant effects of family structure on heavy episodic drinking were found. While family cohesion mediated the effects of family structure on adolescents’ health, the mediating effect of socio-economic status was small. After controlling for pre-transition health, the effects were even lower. Conclusions: Because the direct effects of family structure on adolescents’ health were small and family cohesion was found to be an important mediator in the association between family structure and adolescents’ health, prevention programmes and interventions should be directed towards the parent–adolescent relationship rather than just the family structure, in order to minimize the psychosocial stress of adolescents during the period of family transition.
Efficacy of Using Environmental DNA (eDNA) to Detect Kirtland’s Snakes (Clonophis kirtlandii)
Creators: name: Ratsch, Rikki
Publisher: name: Purdue University Graduate School
Year: 2019
Subjects: Plant cell and molecular biology
Animal cell and molecular biology
Bioinformatic methods development
Bioinformatics and computational biology not elsewhere classified
Other biological sciences not elsewhere classified

description: Environmental DNA (eDNA) surveys utilize DNA shed from animals in order to detect their presence. Since it was developed, this technique has been applied to numerous species across several taxa. In some cases, it has been shown to be superior to traditional survey methods at detecting rare or cryptic species. It allows for the detection of animals in low numbers and does not require direct capture of an animal. This allows eDNA to be more effective at detecting rare or cryptic species that require high survey effort to find. This often reduces survey costs as many eDNA samples can be collected quickly with little equipment required. The Kirtland’s Snake (Clonophis kirtlandii) is a small Natricine snake endemic to the Midwest. It is a species of conservation concern since it is threatened throughout its range. Due to its cryptic and fossorial lifestyle, it is also a notoriously difficult snake to survey. This has resulted in a poor understanding of Kirtland’s Snake life history and population status. Applying eDNA surveys to this species may increase detection probability, offering a more efficient way to survey for them. In this study, a quantitative PCR (qPCR) assay was designed and tested alongside traditional coverboard surveys. The assay had a limit of detection of 166 copies of Kirtland’s Snake DNA. In crayfish burrow sediment, eDNA was found to be detectable up to 10 days and may persist for up to 25 days. However, only one detection occurred out of 380 field samples. Coverboard surveys revealed temporal and spatial variation in Kirtland’s Snake abundance. More snakes were captured in the spring, during the first field season, and at the south coverboard transects. Kirtland’s Snake abundance was also found to be related to the presence of grass and herbaceous vegetation as well as close proximity to shrubs. Comparing survey methods, coverboards resulted in far better snake detection, suggesting that eDNA does not offer an advantage over traditional survey methods for this species.
FEEDING DEVELOPMENT IN THE CONTEXT OF INFANT VOCALIZATIONS & CAREGIVER FEEDING RESPONSIVENESS
Creators: name: Hahn Arkenberg, Rachel
name: Malandraki, Georgia A
name: Seidl, Amanda
name: Hustad, Katherine C.
name: Moding, Kameron
name: Delaney, Amy L.
name: Schaser, Allison J.
Publisher: name: Purdue University Graduate School
Year: 2024
Subjects: classificationCode: 420110
subject: Speech pathology

description: The development of feeding skills is essential for infant health, growth, and family well-being. Despite the importance of this skill, relatively little is known about the development of independent solid feeding skills relative to other body systems – like vocalizations – or external factors including caregivers or the feeding environment. The purpose of this preliminary study was to examine the relationship between feeding skills, vocalizations, and caregiver feeding responsiveness at the same point in 6-8-month-old infants. We conducted this study remotely in order to obtain the most accurate assessment of infant skills and include diverse infant feeding experiences within their own home environments. Twenty-five typically developing – low-risk – infants and a pilot group of ten infants at increased risk for feeding and communication disorders completed the study, along with their caregivers. Infants were categorized as “at-risk” if they spent time in the Neonatal Intensive Care Unit. We collected feeding data through caregiver-recorded videos of typical mealtimes, vocalization measures from daylong audio recordings, and responsiveness assessments from video-conferencing interviews with caregivers and the mealtime feeding videos. In our sample, it was feasible to use these remote data collection methods, and we obtained high inter- and intra-rater reliability for all measures (> 90%). In our preliminary study, we found that infants in the low- and at-risk groups demonstrated different oral motor feeding skills. In hierarchical regression models, the interaction between risk group and utterance duration was the most significant predictor of oral motor feeding skills, while neither vocalization measure was strongly related to eating efficiency. In the opposite direction, feeding skills were not predictive of either normalized child vocalization count or utterance duration. Relative to caregiver feeding responsiveness, we found that caregiver feeding responsivity alone did not predict feeding or vocalization measures. Responsivity in combination with weeks of feeding experience and medical risk group was related to oral motor feeding skill but not related to vocalization measures. Overall, this study provided initial evidence that feeding skills develop as a dynamic system, influenced by multiple within-child and external factors, and future research is warranted on the influence of these factors on feeding and communication skill development.
Development of an Environmental DNA Assay for Eastern Massasauga
Creators: name: Merkling, Jessica
Publisher: name: Purdue University Graduate School
Year: 2019
Subjects: Other biological sciences not elsewhere classified
Plant cell and molecular biology
Animal cell and molecular biology
Other environmental sciences not elsewhere classified

description: Utilizing environmental DNA (eDNA) for the detection of species in the field is a potentially cost-effective and time-saving technology that may be useful in understanding the distribution and abundance of threatened or endangered species such as the Eastern Massasauga (Sistrurus catenatus). I describe the development of an eDNA assay for the species and evaluate its ability to detect eDNA in laboratory and field conditions. In the field samples, I also investigated the potential for abiotic conditions to influence eDNA detection. Species-specific primers and probe were designed to amplify a 152 bp segment of the massasauga COI gene. Target eDNA could be detected in samples containing as little as 100 copies of target DNA/μL. Water samples collected from laboratory housed snakes indicated that eDNA can be detected in water 56 days after massasauga removal. Field samples were taken from crayfish burrows, known overwintering habitat for the species, from four sites that vary in snake use as ascertained by traditional visual surveys. Of the 60 burrows sampled, seven had a positive detection for massasauga eDNA with no difference in detection rate between DNA extracted from burrow water and burrow sediment. Occupancy models fitted to burrow water indicated that larger amounts of total DNA in a sample may increase the probability of detection of a massasauga eDNA. Large confidence intervals in site occupancy (ѱ) and burrow detection (Θ) values suggest that a larger sample size is needed for more reliable occupancy models. Abiotic conditions within crayfish burrows varied among sites but correlation with eDNA detection was not supported. Estimates of qPCR detection within a burrow with eDNA (ρ) suggest that up to 10 qPCR replicates per burrow sample may be necessary. Further studies need to examine eDNA degradation in the field, improve upon the limit of detection, and sample a larger number of sites for eDNA sampling to be a stand-alone survey method for Eastern Massasaugas.
Additional file 6 of DNA methylation analysis identifies key transcription factors involved in mesenchymal stem cell osteogenic differentiation
Creators: name: Gómez, Rodolfo
name: Barter, Matt J.
name: Alonso-Pérez, Ana
name: Skelton, Andrew J.
name: Proctor, Carole
name: Herrero-Beaumont, Gabriel
name: Young, David A.
Publisher: name: figshare
Year: 2024
Subjects: subject: Genetics
subject: FOS: Biological sciences

description: Additional file 6: Figure S1. Observed vs expected ratio of CpGs enrichment among the different chromatin states and their cumulative methylation profile in MSCs. A) The bars represent the ratio of the percentage of differently methylated CpGs vs the percentage of the total CpGs studied for each chromatin state. B) Cumulative plot of the methylation of the CpGs overlapping top enriched (Strong_ Enhancer, Weak_ Enhancer, and Txn_Transition) and depleted chromatin states (Active_promoter and Poised_ Promoter). Y axis: cumulative percentage of CpGs overlapping to each chromatin state. X axis: fold change methylation MSCs vs Osteoblasts.
Additional file 1 of Anti-inflammatory and anti-oxidative electrospun nanofiber membrane promotes diabetic wound healing via macrophage modulation
Creators: name: He, Jibing
name: Zhou, Shasha
name: Wang, Jiaxing
name: Sun, Binbin
name: Ni, Dalong
name: Wu, Jinglei
name: Peng, Xiaochun
Publisher: name: figshare
Year: 2024
Subjects: subject: Biochemistry
subject: Space Science
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Pharmacology
subject: Chemical Sciences not elsewhere classified
subject: Immunology
subject: FOS: Clinical medicine
subject: Infectious Diseases
subject: FOS: Health sciences

description: Supplementary Material 1
TRANSIENT OPERATION AND SIMULATION OF A FLAT PLATE SOLAR COLLECTOR WITH TANK AND THERMAL STORAGE
Creators: name: Kurri, Manikanta
Publisher: name: Purdue University Graduate School
Year: 2019
Subjects: Mechanical engineering not elsewhere classified

description: Global warming due to over usage of fossil fuels is leading to different kinds of pollutions. Global warming can be controlled by switching non-renewable fossil fuels with renewable alternative power sources like wind, solar, geothermal, biomass, and ocean. Solar power is the most prominent alternative source around the world. Solar power is being used as alternative power source in almost all countries around the globe. Solar power uses radiation from sun to produce heat and the heat used for different heating and cooling applications whereas electricity being one of them. Solar power is being used in domestic applications as water heaters. A solar collector is used to transfer energy from sun to heat water. A flat plate solar collector is commonly used for water heating systems. Solar radiation is not available entire day as other alternative energy sources so there may be a need to store the collected radiation using a thermal storage system. This study considers a solar collector system with a storage tank and phase change material (PCM). The simulation of a transient process using one-dimensional mathematical model for the collector and storage system with phase change materials (PCMs) is presented. The collector used in this study is a flat plate model and paraffin wax encapsulated in aluminum cylinders as phase change material (PCM). The thermal energy gained by solar radiation can be stored for longer period in a system which has phase change materials (PCMs) due to latent heat storage or enthalpy of fusion.
Modeling and Simulation of Heat Pump Systems Combined with Solar Photovoltaic
Creators: name: Busineni, Vijaya Shyam
Publisher: name: Purdue University Graduate School
Year: 2019
Subjects: Mechanical engineering not elsewhere classified

description: Renewable energy systems have received considerable attention as a sustainable technology in the building sector. Specifically, the use of ground-source heat pump (GSHP) and air-source heat pump (ASHP) for heating and cooling of buildings is increasing rapidly, and the combination with photovoltaic (PV) systems and heat pump systems provide energy savings and environmental benefits. This study investigates the feasibility of replacing conventional heating and cooling systems in a multifamily, residential building with GSHP and ASHP systems and their combination with PV. The integration of PV with GSHP and ASHP systems presents an opportunity for increased solar energy usage resulting in a reduction of electricity demanded and a reduction of emissions of greenhouse gases. To analyze different heat pumps systems with and without PV, system modeling and computer simulations are performed with RETScreen Expert software.
A multifaceted verification and validation study is conducted for the system model and computer simulation. The important objective of this part of the study is to understand and develop confidence for modelling individual studies in RETScreen Expert software. To accomplish this, RETScreen Expert is used for modeling and simulating the performance of PV systems in several geographical locations, including Fort Wayne, IN. A comparison is made to performance predictions from System Advisory Model (SAM) software. In addition, a study is done to compare predictions from both software to previously published data.

In the further phase of the study, eQUEST software, a tool for building energy simulation is used to predict outputs such as electricity consumption, heating loads, and cooling loads for the multifamily residential building considered in this study. These outputs, as well as, building parameters are used as inputs to RETScreen Expert. Since, this study focuses on modeling and simulating the heating and cooling systems coupled with PV for feasibility analysis, only a few minor modifications to the eQUEST default settings are made.

The outputs from eQUEST are used as inputs to RETScreen Expert and analysis of ASHP and GSHP systems, as well as their combination with a PV system are performed. The results include the technical performance and financial model of each system, which can be used to indicate feasibility. The results show that both GSHP and ASHP systems are environmentally friendly and reduce energy consumption. These systems are economically feasible, with payback periods of under 10 years, when electricity prices are high. When combined technology is preferred, PV-GSHP systems are more environmentally friendly and have fuel savings far better than any other proposed systems. But the feasibility of the both the GSHP and PV-GSHP systems strongly depends on loop installation cost.
sj-docx-3-asw-10.1177_11786221211028162 – Supplemental material for Effective Chromium Adsorption From Aqueous Solutions and Tannery Wastewater Using Bimetallic Fe/Cu Nanoparticles: Response Surface Methodology and Artificial Neural Network
Creators: name: Mahmoud, Ahmed S.
name: Mohamed, Nouran Y.
name: Mostafa, Mohamed K.
name: Mahmoud, Mohamed S.
Publisher: name: SAGE Journals
Year: 2021
Subjects: subject: 69999 Biological Sciences not elsewhere classified
subject: FOS: Biological sciences

description: Supplemental material, sj-docx-3-asw-10.1177_11786221211028162 for Effective Chromium Adsorption From Aqueous Solutions and Tannery Wastewater Using Bimetallic Fe/Cu Nanoparticles: Response Surface Methodology and Artificial Neural Network by Ahmed S. Mahmoud, Nouran Y. Mohamed, Mohamed K. Mostafa and Mohamed S. Mahmoud in Air, Soil and Water Research
Additional file 8 of DNA methylation analysis identifies key transcription factors involved in mesenchymal stem cell osteogenic differentiation
Creators: name: Gómez, Rodolfo
name: Barter, Matt J.
name: Alonso-Pérez, Ana
name: Skelton, Andrew J.
name: Proctor, Carole
name: Herrero-Beaumont, Gabriel
name: Young, David A.
Publisher: name: figshare
Year: 2024
Subjects: subject: Genetics
subject: FOS: Biological sciences

description: Additional file 8: Figure S3. Validation of REINS algorithm. Transcription factor relevance (TFR) metric for hypo (Ho)- and hypermethylated (Hr) CpGs associated to MSCs differentiation to osteoblasts (OB), to different tissues (muscle, adipose tissue, pancreas, thymus) and to human pluripotent stem cells (hPSC). A) TFR for the repressor TF CTBP2. B) TFR for the TF EZH2 a member of the polycomb repressive complex 2 (PRC2). C) TFR for the TF SUZ12 a member of the polycomb repressive complex 2 (PRC2). D) TFR for the TF ZEB1.
Linearizing E- Class Power Amplifier by Using Memoryless Pre-Distortion
Creators: name: Dey, Tunir
Publisher: name: Purdue University Graduate School
Year: 2020
Subjects: Electrical engineering not elsewhere classified

description: Radio Frequency Power Amplifiers (PA) are essential components of wireless systems and nonlinear in a permanent way. So, high efficiency and linearity at a time are imperative for power amplifiers. However, it is hard to obtain because high efficiency Power Amplifiers are nonlinear and linear Power Amplifiers have poor efficiency. To meet both linearity and efficiency, the linearization techniques such as Digital Predistortion (DPD) has arrested the most attention in industrial and academic sectors due to provide a compromising data between efficiency and linearity. This thesis proposed on digital predistortion techniques to control nonlinear distortion in radio frequency transmitters. By using predistortion technique, both linearity and efficiency can obtain. In this thesis a new generic Saleh model for use in memoryless nonlinear power amplifier (PA) behavioral modelling is used. The results are obtained by simulations through MATLAB and experiments. We explore the baseband 13.56 MHz Power Amplifier input and output relationships and reveal that they apparent differently when the Power Amplifier shows long-term, short-term or memory less effects. We derive a SIMULINK based static DPD design depend on a memory polynomial. A polynomial improves both the non-linearity and memory effects in the Power Amplifier. As PA characteristics differs from time to time and operating conditions, we developed a model to calculate the effectiveness of DPD. We extended our static DPD design model into an adaptive DPD test bench using Indirect Learning Architecture (ILA) to implement adaptive DPD which composed of DPD subsystem and DPD coefficient calculation. By this technique, the output of PA achieves linear, amplitude and phase distortions are eliminated, and spectral regrowth is prevented. The advanced linearity performance executed through the strategies and methods evolved on this thesis can allow a higher usage of the capability overall performance of existing and emerging exceptionally performance PAs, and therefore an anticipated to have an effect in future wireless communication systems.
DARSDAN TASHQARI MASHG'ULOTLARNI O'TKAZISH SSENARIYLARI BILAN TANISHISH
Creators: name: Nadirov, Obodjon
name: Yaxiyaxonova, Muxiba
Publisher: name: Zenodo
Year: 2025

description: Mazkur maqolada darsdan tashqari mashg‘ulotlarning o‘quvchilarning bilim olish jarayonidagi o‘rni va ahamiyati tahlil qilinadi. Ta’lim jarayoni faqat sinf xonasida emas, balki real hayotiy sharoitlarda ham davom etishi lozimligi ta’kidlangan. J. Dewey, V. A. Sukhomlinskiy, R. G‘ofurov va A. Qo‘chqorov kabi pedagoglarning fikrlariga asoslanib, darsdan tashqari faoliyat o‘quvchilarning mustaqil fikrlashini rivojlantirish, ijodiy qobiliyatlarini oshirish va amaliy ko‘nikmalarini shakllantirish uchun muhim vosita ekanligi yoritiladi.
Additional file 1 of Understanding nonlinear and synergistic effects of the built environment on urban vibrancy in metro station areas
Creators: name: Peng, Jiandong
name: Hu, Yiwen
name: Liang, Chao
name: Wan, Qiuyu
name: Dai, Qi
name: Yang, Hong
Publisher: name: figshare
Year: 2024
Subjects: subject: Space Science
subject: Medicine
subject: Biotechnology
subject: Ecology
subject: FOS: Biological sciences
subject: Biological Sciences not elsewhere classified
subject: Mathematical Sciences not elsewhere classified
subject: Science Policy

description: Additional file 1: Literature review. Table S1. Recent studies incorporating diverse proxies for urban vibrancy.