cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Self-dual SU(2) invariant Einstein metrics and modular dependence of theta-functions
Creators: name: Babich, M. V.
name: Korotkin, D. A.
Publisher: name: arXiv
Year: 1998
Subjects: subject: General Relativity and Quantum Cosmology (gr-qc)
subject: Differential Geometry (math.DG)
subject: Exactly Solvable and Integrable Systems (nlin.SI)
subject: FOS: Physical sciences
subject: FOS: Mathematics

description: We simplify Hitchin's description of SU(2)-invariant self-dual Einstein metrics, making use of the tau-function of related four-pole Schlesinger system.
description: Misprints corrected
Activation of latent Epstein-Barr virus genomes: selective stimulation of synthesis of chromosomal proteins by a tumor promoter.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1983

description: The tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA) is a potent inducer of Epstein-Barr virus (EBV) gene expression. The optimal conditions for maximum activation of latent EBV genomes by TPA were determined. Although TPA is able to induce replication of EBV genomes in P3HR-1 cells in all phases of growth, the greatest increase in viral genome copies per cell (15-fold above the control level) occurred in nonproliferating cells as opposed to cells growing exponentially (6-fold above the control level). The synthesis of chromosomal proteins in nonproliferating cells under the conditions that induce maximum activation of latent virus genomes by TPA was studied. Selective stimulation in chromosomal protein synthesis accompanied the increase in EBV genomes in P3HR-1 cells despite an overall reduction in total cellular protein synthesis. Comparison of the chromosomal proteins from TPA-induced P3HR-1 cells and from superinfected Raji cells revealed comigrating chromosomal polypeptides of 145K, 140K, 135K, 110K, 85K, and 55K that are presumably EBV associated. The selective stimulation of synthesis of these chromosomal proteins in TPA-treated P3HR-1 cells was closely associated with the activation of latent EBV genomes.
Tidal disruption events from a nuclear star cluster as possible origin of transient relativistic spectral lines near SMBH
Creators: name: Karas, V.
name: Dovciak, M.
name: Kunneriath, D.
name: Yu, W.
name: Zhang, W.
Publisher: name: arXiv
Year: 2014
Subjects: subject: High Energy Astrophysical Phenomena (astro-ph.HE)
subject: FOS: Physical sciences

description: We discuss a possibility that a tidal disruption event near a dormant supermassive black hole (SMBH) can give rise to spectral features of iron in 6-7 keV X-ray signal: a relativistic line profile emerges from debris illuminated and ionised by an intense flash produced from the destroyed star. This could provide a unique way to determine parameters of the system. We consider a model where the nuclear stellar population acquires an oblate shape (i.e., a flattened distribution) in the inner region near a supermassive black hole, and also the primary irradiation flare is expected to occur more likely near the equatorial plane, co-planar with the infalling material. This suggests that the reprocessing of primary X-rays results in a transient profile that should be relevant for tidal-disruption events (TDE) in otherwise under-luminous (inactive) galactic nuclei, i.e. with no prior accretion disc. Resonance mechanisms of the stellar motion can increase the orbital eccentricity for some stars in the nuclear cluster and help to bring them close to the tidal radius, where they can give rise to TDEs. The proposed scenario appears to be distinguishably different from the standard scheme of lamp-post model for the origin of the relativistic line in active galaxies, where the source is thought to be located predominantly near the symmetry axis.
description: 8 pages, 2 figures, submitted to the Proceedings of RAGtime: Workshops on black holes and neutron stars
Cell-free synthesis and assembly of vesicular stomatitis virus nucleocapsids.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1983

description: The association of newly synthesized vesicular stomatitis virus proteins into nucleocapsid structures was examined in a cell-free system that supports concurrent viral protein synthesis, transcription, and RNA replication. The vesicular stomatitis virus proteins synthesized by this system associated with the newly replicated RNA to form structures that banded in CsCl gradients with marker nucleocapsids. In reactions lacking nucleocapsid templates to program RNA synthesis, the newly synthesized proteins did not associate into nucleocapsid structures. The newly synthesized proteins associated with nucleocapsids were analyzed by electrophoresis on polyacrylamide gels containing sodium dodecyl sulfate after separation from non-associated proteins by chromatography on Bio-Gel A15M agarose columns. The results of this analysis showed that newly synthesized L, NS, and N proteins associated into nucleocapsids in the in vitro system. In addition, a small amount of newly synthesized M protein was stably bound to the nucleocapsids. The molar ratio of the associated, newly synthesized proteins was 2:350:1,000:10 (L:NS:N:M). More than 90% of the newly synthesized NS protein that associated with nucleocapsids in vitro was of the NS2 subspecies, as assayed by DEAE-cellulose column chromatography. The stability of the association of the newly synthesized proteins with nucleocapsids in the system mimicked that of the association of viral proteins with nucleocapsids from infected cells as measured by salt sensitivity. These data indicate that nucleocapsids were assembled from newly synthesized proteins within our in vitro system and that the molar ratio of assembled proteins was similar to that observed for virion nucleocapsids.
Epstein-Barr virus transcription in nasopharyngeal carcinoma.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1983

description: Sequences which encode Epstein-Barr virus (EBV) RNA in nasopharyngeal carcinoma (NPC) tissue have been identified. We utilized human biopsy material directly as well as NPC grown in nude mice. Total RNA was extracted from the tumor material and separated into polyadenylated and nonpolyadenylated fractions by oligodeoxythymidylate-cellulose chromatography. This material was used as template to construct 32P-labeled cDNA. The labeled cDNAs were hybridized to Southern blots of recombinant EBV DNA fragments. Three of the biopsies, F, 49, and 55, contained polyadenylated RNA homologous to the EBV BamHI fragments V and K, and EcoRI-DIJhet. These same fragments encode the most abundant polyribosomal RNAs in latently infected lymphoblastoid cell lines. The sequences which encoded nonpolyadenylated RNA in NPC tumor 49 were more extensive and included BamHI fragments C, V, B, E, and K, and EcoRI fragments DIJhet, E, F, and G1, a result that indicates selective polyadenylation in EBV RNA processing. A fourth biopsy, NPC tumor 18, contained polyadenylated RNA homologous to the BamHI fragments H, B, K, Y, B1, I1, and A and EcoRI fragments F and G2. A similar pattern of transcription was identified in three tumor specimens from nude mice, 4, 5, and 8. Transformation of lymphocytes did not occur after cocultivation in vitro with explants from these nude mice tumors. This transcriptional pattern may represent an activated state of the EBV genome, formerly not detected in tumor tissue, which is analogous to the state of abortive infection identified in induced in vitro cell systems.
Replication of vesicular stomatitis virus defective interfering particle RNA in vitro: transition from synthesis of defective interfering leader RNA to synthesis of full-length defective interfering RNA.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1983

description: The replication of the RNA of vesicular stomatitis virus (VSV) defective interfering (DI) particles was established in a defined cell-free system. The transition from synthesis of only the DI-leader RNA to replication of the full-length DI RNA was effected in the system by newly synthesized VSV proteins and occurred in the absence of VSV helper virus. Both positive- and negative-polarity full-length DI RNA were synthesized. Furthermore, the products of RNA replication associated with newly synthesized viral proteins to form complexes that were indistinguishable from authentic DI particle nucleocapsids on the basis of buoyant density and resistance to ribonuclease digestion. The DI-leader RNA did not form ribonuclease-resistant structures. We conclude that this in vitro system successfully executes many of the reactions of VSV DI particle replication and assembly.
Mapping and expression of a human cytomegalovirus major viral protein.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1984

description: We constructed a DNA fragment map of low-passage Towne strain cytomegalovirus by analyzing cross-blot hybridization and hybridizations of isolated recombinant clones. The abundant late transcripts were located on this map by hybridization of labeled total RNA of virus-infected cells to blotted DNA fragments. The most abundant late transcript, carried by the 11.7-kilobase EcoRI fragment (EcoRI-G), was precisely mapped. The EcoRI fragment was fragmented and subcloned in a plasmid carrying simian virus 40 sequences (pSV-OH, constructed by Chi-Bom Chae, Department of Biochemistry, University of North Carolina, Chapel Hill). One resulting recombinant plasmid, pHD713SV2, was transferred to simian virus 40-transformed monkey kidney cells (COS-1) by DNA transfection. Synthesis of a cytomegalovirus-specific 67-kilodalton protein was detected in these cells by reaction of blotted proteins with virus-specific monoclonal antibody. The 67-kilodalton protein is a major phosphorylated protein found in virions; it is not glycosylated. The location of the gene for this 67-kilodalton protein is therefore assigned to the center of the L-unique region of human cytomegalovirus, at 0.37 to 0.39 map units.
EOSC Support Office Austria 2nd General Assembly Slide Deck
Creators: name: Hasani-Mavriqi, Ilire
Publisher: name: Zenodo
Year: 2022

description: This upload contains the slide deck used at the European Open Science Cloud (EOSC) Support Office Austria second General Assembly. The European Open Science Cloud (EOSC) Support Office Austria, the Austrian EOSC Mandated Organisation, cordially invited to its second General Assembly. This time it took place at the Old Campus (Alte Technik) of the Graz University of Technology. In this meeting one year of collaboration was celebrated, the work carried out and the goals already achieved reflected and the vision for the future and further goals of EOSC in Austria presented. Additionally, there were keynote speakers from the EOSC Association and EOSC-related projects to describe current development in the European Open Science Cloud ecosystem.
Viscous Quark-Gluon Plasma Model Through Fluid QCD Approach
Creators: name: Djun, T. P.
name: Soegijono, B.
name: Mart, T.
name: Handoko, L. T.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Nuclear Theory (nucl-th)
subject: High Energy Physics - Phenomenology (hep-ph)
subject: FOS: Physical sciences

description: A Lagrangian density for viscous quark-gluon plasma has been constructed within the fluid-like QCD framework. Gauge symmetry is preserved for all terms inside the Lagrangian, except for the viscous term. The transition mechanism from point particle field to fluid field, and vice versa, is discussed. The energy momentum tensor that is relevant for the gluonic plasma having the nature of fluid bulk of gluon sea is derived within the model. By imposing conservation law in the energy momentum tensor, shear viscosity appears as extractable from the equation.
description: 7 pages, AIP Conference Proceeding 1617 (2014) 91
N protein alone satisfies the requirement for protein synthesis during RNA replication of vesicular stomatitis virus.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1984

description: Genomic replication of the negative-strand RNA viruses is dependent upon protein synthesis. To examine the requirement for protein synthesis in replication, we developed an in vitro system that supports the genome replication of defective interfering particles of the negative-strand rhabdovirus vesicular stomatitis virus (VSV), as a function of protein synthesis (Wertz, J. Virol. 46:513-522, 1983). The system consists of defective interfering nucleocapsid templates and an mRNA-dependent reticulocyte lysate to support protein synthesis. We report here an analysis of the requirement for individual viral proteins in VSV replication. Viral mRNAs purified by hybridization to cDNA clones were used to direct the synthesis of individual proteins in the in vitro system. By this method, it was demonstrated that the synthesis of the VSV nucleocapsid protein, N, alone, resulted in the replication of genome-length RNA by both defective interfering intracellular nucleocapsids and virion-derived nucleocapsids. Neither the viral phosphoprotein, NS, nor the matrix protein, M, supported RNA replication. The amount of RNA replication for a given amount of N protein was the same in reactions in which either all of the VSV proteins or only N protein were synthesized. In addition, RNA replication products synthesized in reactions containing only newly made N protein assembled with the N protein to form nucleocapsids. These results demonstrate that the major nucleocapsid protein (N) can by itself fulfill the requirement for protein synthesis in RNA replication and allow complete replication, i.e., initiation and elongation, as well as encapsidation of genome-length progeny RNA.
A new method to estimate local pitch angles in spiral galaxies: Application to spiral arms and feathers in M81 and M51
Creators: name: Puerari, Ivânio
name: Elmegreen, Bruce G.
name: Block, David L.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Astrophysics of Galaxies (astro-ph.GA)
subject: FOS: Physical sciences

description: We examine $8μ$m IRAC images of the grand design two-arm spiral galaxies M81 and M51 using a new method whereby pitch angles are locally determined as a function of scale and position, in contrast to traditional Fourier transform spectral analyses which fit to average pitch angles for whole galaxies. The new analysis is based on a correlation between pieces of a galaxy in circular windows of $(\ln R, θ)$ space and logarithmic spirals with various pitch angles. The diameter of the windows is varied to study different scales. The result is a best-fit pitch angle to the spiral structure as a function of position and scale, or a distribution function of pitch angles as a function of scale for a given galactic region or area. We apply the method to determine the distribution of pitch angles in the arm and interarm regions of these two galaxies. In the arms, the method reproduces the known pitch angles for the main spirals on a large scale, but also shows higher pitch angles on smaller scales resulting from dust feathers. For the interarms, there is a broad distribution of pitch angles representing the continuation and evolution of the spiral arm feathers as the flow moves into the interarm regions. Our method shows a multiplicity of spiral structures on different scales, as expected from gas flow processes in a gravitating, turbulent and shearing interstellar medium. We also present results for M81 using classical 1D and 2D Fourier transforms, together with a new correlation method, which shows good agreement with conventional 2D Fourier transforms.
description: 25 pages, 12 figures, Accepted to The Astronomical Journal
Prolonged inhibitory effect of 9-(1,3-dihydroxy-2-propoxymethyl)guanine against replication of Epstein-Barr virus.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1984

description: The effects of 9-(1,3-dihydroxy-2-propoxymethyl)guanine (DHPG), a new antiviral drug, and acyclovir (ACV) [9-(2-hydroxyethoxymethyl)guanine] on the replication of Epstein-Barr virus (EBV) were compared. Both drugs inhibited EBV DNA replication in P3HR-1 cells and superinfected Raji cells, but neither inhibited replication of the plasmid form of the EBV genome in latently infected Raji cells. However, DHPG had a more prolonged inhibitory effect than ACV. Although the effect of the drugs is prompt, the kinetics of inhibition of EBV replication indicated that a drug exposure of 14 days was needed to reduce the EBV genome copy number to the residual plasmid level (30 copies per cell). The inhibitory effect of ACV was readily reversed within 11 days after removal of the drug, in contrast to the more prolonged effect exerted by DHPG, which persisted for more than 21 days. The 50% inhibitory doses for cell growth of ACV and DHPG were estimated to be 250 and 200 microM, respectively. The viral 50% and 90% effective doses of inhibition were, respectively, 0.3 and 9 microM for ACV and 0.05 and 3 microM for DHPG. The therapeutic indices (50% inhibitory dose/50% effective dose) for ACV and DHPG were 833 and 4,000, respectively. Synthesis of EBV-associated polypeptides was also affected. In superinfected Raji cells, ACV (100 microM) and DHPG (30 microM) inhibited synthesis of polypeptides with molecular weights of 145,000 and 140,000; in addition, synthesis of polypeptides with molecular weights of 110,000 and 85,000 was markedly reduced by DHPG but not by ACV. However, after drug removal, the inhibitory effect of ACV on polypeptide synthesis was abolished in contrast to the more persistent effect of DHPG.
Transcriptional mapping of human respiratory syncytial virus.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1984

description: A transcriptional map for human respiratory syncytial virus was determined by measuring the kinetics of viral gene inactivation in response to UV irradiation. Monolayer cell cultures of respiratory syncytial virus-infected HEp-2 cells were exposed to UV light, and residual viral RNA synthesis was monitored both by gel electrophoresis and by hybridization to dot blots of cloned cDNAs of the 10 known viral genes. Target sizes for the 10 individual viral genes were calculated relative to the UV sensitivity of intracellular viral genome replication. Target size analysis indicated that the 10 viral genes were transcribed as a single transcriptional unit and that the transcription of an individual gene was dependent on the prior transcription of all the preceding genes. The order of gene transcription (with nomenclature according to encoded proteins) was determined to proceed from the promoter as follows: 14K, 11K, N, P, M, 9.5K, 36K, F, 24K, L.
A new pathway in the generation of defective retrovirus DNA.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1985

description: We used a retrovirus shuttle vector to make molecular clones of circular viral DNA from infected cells. One-third of the molecules examined had deletions that started within or near the U5 domain of the long terminal repeat (LTR) region and extended a variable distance toward the gag gene. We present evidence that some of these deletions arose by cleavage of a single LTR unit, in contrast to the cleavage of tandem LTR units associated with the integration reaction. These results suggest that in the formation of defective circular DNA, the U5 domain can be recognized and cleaved in the absence of an adjacent U3 domain. The cleavage of isolated U5 domains may represent an important mechanism responsible for the generation of certain forms of both defective circular DNA and defective integrated DNA.
Development and Evaluation of Innovative Stationary Phases for Separation of Pharmaceuticals, Metabolites and Biocompounds
Creators: name: Grasso, Giuliana
Publisher: name: Universita degli studi di Salerno
Year: 2020
Subjects: subject: CHIM/01 CHIMICA ANALITICA
subject: Lipids
subject: Biofluids detection
subject: Selective materiales

description: 2018 - 2019
description: Biofluids are typical complex matrices representing a source of potential biomarkers, but often require high demands in sample preparation prior to analysis. With this purpose, several techniques have been developed including solid phase extraction and methods based on the molecular recognition, such as immuno-affinity sorbents and molecularly imprinted polymers. Lipidomics is a research field in which it is needed to develop efficient techniques for sample pre-treatment in order to obtain reliable and reproducible results. Lipids are involved in many processes, as cellular structures, signaling, within the cell and between cells, and energy storage. Because of the role of lipids and all their functions, the field of Lipidomics is emerging with the focus on identifying alterations in lipids metabolism and lipid-mediated signalling processes that regulate cellular homeostasis and trying to understand the relationship between these processes in health and disease (Han & Gross, 2003). .. [edited by the Author]
Accumulation of herpes simplex virus type 1 RNAs of different kinetic classes in the cytoplasm of infected cells.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1985

description: On a déterminé les différents types d'accumulation des RNAs du virus herpès simplex de type 1 dans les cytoplasmes de cellules infectées en présence de drogues et surtout d'une infection normale. On a pu établir une comparaison entre l'accumulation des RNAm de IE (ICP4 et IICPO), de E(TK) et L(ICP-5) qui codent par des protéines de classes cinétiques différentes. Des contrôles de la traduction peuvent s'exercer dans la régulation des gènes IE mais pas sur les gènes précoces et tardifs.
DNA packaging by the Bacillus subtilis defective bacteriophage PBSX.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1985

description: Defective bacteriophage PBSX, a resident of all Bacillus subtilis 168 chromosomes, packages fragments of DNA from all portions of the host chromosome when induced by mitomycin C. In this study, the physical process for DNA packaging of both chromosomal and plasmid DNAs was examined. Discrete 13-kilobase (kb) lengths of DNA were packaged by wild-type phage, and the process was DNase I resistant and probably occurred by a head-filling mechanism. Genetically engineered isogenic host strains having a chloramphenicol resistance determinant integrated as a genetic flag at two different regions of the chromosome were used to monitor the packaging of specific chromosomal regions. No dramatic selectivity for these regions could be documented. If the wild-type strain 168 contains autonomously replicating plasmids, especially pC194, the mitomycin C induces an increase in size of resident plasmid DNA, which is then packaged as 13-kb pieces into phage heads. In strain RB1144, which lacks substantial portions of the PBSX resident phage region, mitomycin C treatment did not affect the structure of resident plasmids. Induction of PBSX started rolling circle replication on plasmids, which then became packaged as 13-kb fragments. This alteration or cannibalization of plasmid replication resulting from mitomycin C treatment requires for its function some DNA within the prophage deletion of strain RB1144.
Effects of fermion exchanges on the polarization of exciton condensates
Creators: name: Combescot, M.
name: Combescot, R.
name: Alloing, M.
name: Dubin, F.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Quantum Gases (cond-mat.quant-gas)
subject: FOS: Physical sciences

description: Exchange processes are responsible for the stability of elementary boson condensates with respect to their possible fragmentation. This remains true for composite bosons when single fermion exchanges are included but spin degrees of freedom are ignored. We here show that their inclusion can produce a "spin-fragmentation" of a condensate of dark excitons, i.e., an unpolarized condensate with equal amount of dark excitons with spins (+2) and (-2). Quite surprisingly, for spatially indirect excitons of semiconductor bilayers, we predict that the condensate polarization can switch from unpolarized to fully polarized, depending on the distance between the layers confining electrons and holes. Remarkably, the threshold distance associated to this switching lies in the regime where experiments are nowadays carried out.
description: 5 pages, 1 figure
Epstein-Barr virus transformation of human B lymphocytes despite inhibition of viral polymerase.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1985

description: Epstein-Barr virus transformed human lymphocytes despite the presence of up to 500 microM acyclovir [9-(2-hydroxyethoxymethyl)guanine], a viral DNA polymerase inhibitor. The transformed cells contained multiple Epstein-Barr virus genome copy numbers. Functional viral DNA polymerase is probably not required for cell transformation and the initial amplification of the viral genome.
Infectious hepatitis A virus particles produced in cell culture consist of three distinct types with different buoyant densities in CsCl.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1985

description: Although hepatitis A virus (HAV) released by infected BS-C-1 cells banded predominantly at 1.325 g/cm3 (major component) in CsCl, smaller proportions of infectious virions banded at 1.42 g/cm3 (dense HAV particles) and at 1.27 g/cm3 (previously unrecognized light HAV particles). cDNA-RNA hybridization confirmed the banding of viral RNA at each density, and immune electron microscopy demonstrated apparently complete viral particles in each peak fraction. The ratio of the infectivity (radioimmunofocus assay) titer to the antigen (radioimmunoassay) titer of the major component was approximately 15-fold greater than that of dense HAV particles and 4-fold that of light HAV particles. After extraction with chloroform, the buoyant density of light and major component HAV particles remained unchanged, indicating that the lower density of the light particles was not due to association with lipids. Light particles also banded at a lower density (1.21 g/cm3) in metrizamide than did the major component (1.31 g/cm3). Dense HAV particles, detected by subsequent centrifugation in CsCl, were indistinguishable from the major component when first banded in metrizamide (1.31 g/cm3). However, dense HAV particles recovered from CsCl subsequently banded at 1.37 g/cm3 in metrizamide. Electrophoresis of virion RNA under denaturing conditions demonstrated that dense, major-component, and light HAV particles all contained RNA of similar length. Thus, infectious HAV particles released by BS-C-1 cells in vitro consist of three distinct types which band at substantially different densities in CsC1, suggesting different capsid structures with varied permeability to cesium or different degrees of hydration.
Inhibition of cellular DNA polymerase alpha and human cytomegalovirus-induced DNA polymerase by the triphosphates of 9-(2-hydroxyethoxymethyl)guanine and 9-(1,3-dihydroxy-2-propoxymethyl)guanine.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1985

description: The triphosphates of 9-(2-hydroxyethoxymethyl)guanine and 9-(1,3-dihydroxy-2-propoxymethyl)guanine were examined for their inhibitory effect on highly purified cellular DNA polymerase alpha and human cytomegalovirus (Towne strain)-induced DNA polymerase. These two nucleoside triphosphates competitively inhibited the incorporation of dGMP into DNA catalyzed by the DNA polymerases. The virus-induced DNA polymerase had greater binding affinity for the triphosphate of 9-(2-hydroxyethoxymethyl)guanine (Ki, 8 nM) than for the triphosphate of 9-(1,3-dihydroxy-2-propoxymethyl)guanine (Ki, 22 nM), although the nucleoside of the latter compound was strikingly more effective against human cytomegalovirus replication in cell cultures than the nucleoside of the former. The Ki values of these two nucleoside triphosphates for alpha polymerase were 96 and 146 nM, respectively, and were 7- to 12-fold higher than those for the virus-induced enzyme. These data indicated that virus-induced DNA polymerase was more sensitive to inhibition by these two nucleoside triphosphates than was the cellular alpha enzyme.
Qualitative and quantitative analyses of Epstein-Barr virus early antigen diffuse component by western blotting enzyme-linked immunosorbent assay with a monoclonal antibody.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1985

description: We report the use of monoclonal antibody against the early antigen diffuse component (anti-EA-D) of Epstein-Barr virus (EBV) to analyze, both qualitatively and quantitatively, the expression of EA-D in various human lymphoblastoid cell lines activated by chemical inducers. The kinetics of synthesis of EA-D in P3HR-1, B95-8, and Ramos/AW cells were similar in that they all reached the peak of synthesis on day 5 after induction. Surprisingly, no expression of EA-D was found in induced BJAB/GC, an EBV-genome-containing cell line. EBV-negative cell lines, BJAB and Ramos, were negative for EA-D. Raji cells had no detectable EA-D but responded rapidly to induction, reaching a peak on day 3. Superinfection of Raji cells also resulted in marked induction of EA-D, which reached a plateau between 8 to 12 h postinfection. Western blotting coupled with the enzyme-linked immunosorbent assay was employed to identify polypeptides representing EA-D. A family of four polypeptides with molecular weights of 46,000 (46K protein), 49,000, 52,000, and 55,000 were identified to be reactive with monoclonal anti-EA-D antiserum. The pattern of EA-D polypeptides expressed in each cell line was different. Of particular interest was the expression of a large quantity of 46K protein both in induced Raji and P3HR-1 cells, but not in superinfected Raji cells. A 49K doublet was expressed in activated p3HR-1, B95-8, and Ramos/AW cells and in superinfected Raji cells. In addition, two distinct 52K and 55K polypeptides were expressed in induced Ramos/AW and superinfected Raji cells. However, none of these EA-D polypeptides was detectable in BJAB/GC, BJAB, Ramos, and mock-infected Raji cells. To approximate relative concentrations of EA-D in cell extracts, we employed the enzyme-linked immunosorbent assay and immunoblot dot methods by using one of the purified EA-D components to construct a standard curve. Depending upon the cell lines, it was estimated that ca. 1 to 3% (determined by the enzyme-linked immunosorbent assay) and 0.8 to 1.6% (determined by immunoblot dot) of total proteins from maximally induced cells were EA-D. These results suggest that differential expression of EA-D polypeptides could be of importance in the diagnosis of state of EBV infection.
The potential of mobile exhibition as a form of implementation for social transformation and educational expo-design in addressing public social problems
Creators: name: Nabiyev, Rifkat I.
name: Nabiyev, Ilshat H.
name: Ziatdinov, Rushan
Publisher: name: arXiv
Year: 2014
Subjects: subject: Computers and Society (cs.CY)
subject: FOS: Computer and information sciences

description: The article analyzes the humanistic basis of expo-design in conjunction with the ideological problems of modern design and the causes of their occurrence. It reveals the potential of mobile exhibition for social transformation in terms of its structural and ideological aesthetic content. It substantiates the effectiveness of expo-design expression's artistic and aesthetic means in viewer personality moral education. It investigates the features of social problems design presentation in the unity of the formative and design material components features of mobile exhibition.
Cross-disciplinary survey of access and assessment criteria of "Best Paper" awards - PROTOCOL
Creators: name: Lagisz, Malgorzata
Publisher: name: Open Science Framework
Year: 2022
Subjects: subject: Arts and Humanities
subject: Law
subject: FOS: Law
subject: Life Sciences
subject: Business
subject: Medicine and Health Sciences
subject: Engineering
subject: Social and Behavioral Sciences
subject: Education
subject: Architecture
subject: FOS: Civil engineering
subject: Physical Sciences and Mathematics
subject: cross-disciplinary
subject: meta-science
subject: open science

description: Aims - To examine eligibility and assessment criteria of research publication recognition awards. - To evaluate potential gender and country biases in the lists of past awardees of the above awards. - To communicate the need for improving awards policies and how this can be done – ultimately making individual publication-related awards more equitable.
The envelope-associated 22K protein of human respiratory syncytial virus: nucleotide sequence of the mRNA and a related polytranscript.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1985

description: We recently determined that respiratory syncytial virus (strain A2) encodes a fourth unique envelope-associated virion protein that has molecular weight of approximately 24,000, as estimated by gel electrophoresis. The nucleotide sequence of the mRNA encoding this novel protein has now been determined from five cDNA clones, including three that contain the complete mRNA sequence. The complete mRNA sequence is 957 nucleotides, exclusive of polyadenylate, and contains two partially overlapping open reading frames. The 5'-proximal open reading frame is favored for utilization by the criteria of the location and sequence of its translational start site. Furthermore, the calculated molecular weight of the encoded protein, 22,153, is in agreement with the previous estimate of 24,000 for the authentic protein identified by hybrid selection and in vitro translation. The sequence of the predicted protein, now designated the 22K protein, contains 194 amino acids, is relatively hydrophilic, and appears to be the most basic of the respiratory syncytial virus proteins. The mRNA also contains a second, internal open reading frame which would encode a protein of 90 amino acids. However, no evidence for this translation product is known. The first nine nucleotides in the mRNA sequence, 5'-GGGGCAAAU, are identical to the conserved sequence identified previously at the 5' termini of seven other respiratory syncytial viral mRNAs; the sequence at the 3' end of the 22K mRNA, 5'. . . AGUUAUUU-polyadenylate, contains the elements of the previously identified 3'-terminal consensus sequence for respiratory syncytial virus mRNAs, AGUUAA(N)1-4-polyadenylate (P. L. Collins, Y. T. Huang, and G. W. Wertz, Proc. Natl. Acad. Sci. U.S.A. 81:7683-7687). In addition, we present and describe the intergenic sequence of a dicistronic RNA derived from readthrough of the F and 22K protein genes.
Herpesvirus papio contains a plasmid origin of replication that acts in cis interspecies with an Epstein-Barr virus trans-acting function.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1986

description: Herpesvirus papio (HVP) and Epstein-Barr virus (EBV) are closely related biologically and biochemically; lymphoblastoid cells infected with either virus contain episomal viral DNA. The putative origin of replication for EBV plasmids (oriP) has been assigned to a 1,790-base-pair fragment (cis) in the short unique region of the genome which requires a viral function supplied in trans from elsewhere in the genome (J. Yates, N. Warren, D. Reisman, and B. Sugden, Proc. Natl. Acad. Sci. USA 81:3806-3810, 1984). We report here the identification of the putative origin of replication (cis) in HVP; we assigned it to the HVP EcoRI K fragment. The results indicate that the HVP replication process requires both a cis and a trans-acting function, analogous to that found in EBV.
Proton magnetic resonance imaging with a nitrogen-vacancy spin sensor
Creators: name: Rugar, D.
name: Mamin, H. J.
name: Sherwood, M. H.
name: Kim, M.
name: Rettner, C. T.
name: Ohno, K.
name: Awschalom, D. D.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Mesoscale and Nanoscale Physics (cond-mat.mes-hall)
subject: FOS: Physical sciences

description: Nuclear magnetic resonance (NMR) imaging with nanometer resolution requires new detection techniques with sensitivity well beyond the capability of conventional inductive detection. Here, we demonstrate two dimensional imaging of $^1$H NMR from an organic test sample using a single nitrogen-vacancy center in diamond as the sensor. The NV center detects the oscillating magnetic field from precessing protons in the sample as the sample is scanned past the NV center. A spatial resolution of 12 nm is shown, limited primarily by the scan accuracy. With further development, NV-detected magnetic resonance imaging could lead to a new tool for three-dimensional imaging of complex nanostructures, including biomolecules.
description: Main text: 19 pages including 5 figures Supplementary Information: 9 pages including 3 figures
Human respiratory syncytial virus glycoprotein G expressed from a recombinant vaccinia virus vector protects mice against live-virus challenge.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1986

description: Recombinant vaccinia virus vectors were constructed which expressed the major surface glycoprotein G of human respiratory syncytial (RS) virus. The biological activity of the G protein expressed from these vectors was assayed. Inoculation of rabbits with live recombinant virus induced high titers of antibody which specifically immunoprecipitated RS virus G protein and was capable of neutralizing RS virus infectivity. Immunization of mice by either the intranasal or the intraperitoneal route with recombinant virus that expressed only the G protein resulted in complete protection of the lower respiratory tract upon subsequent challenge with live RS virus.
Metabolic activation of 9([2-hydroxy-1-(hydroxymethyl)ethoxy]methyl)guanine in human lymphoblastoid cell lines infected with Epstein-Barr virus.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1986

description: 9-([2-Hydroxy-1-(hydroxymethyl)ethoxy]methyl)guanine (BW B759U) is more potent and has a more prolonged inhibitory effect against Epstein-Barr virus (EBV) in vitro than does acyclovir (ACV). To assess the mechanism of this difference, we first compared the extent of phosphorylation of the two drugs in superinfected Raji cells. BW B759U is phosphorylated to levels 100-fold higher than is ACV. In addition, lower levels of phosphorylation of BW B759U and ACV were observed in uninfected Raji cells. Studies on the kinetics of formation of BW B759U triphosphate in superinfected Raji cells indicated that drug-phosphorylating activity was detected as early as 3 h after superinfection; this activity was steadily maintained for the first 7 h, followed by a burst of activity between 7 and 10 h and a doubling of phosphorylation between 10 and 25 h. During the superinfection cycle, the pool sizes of deoxyribonucleoside and ribonucleoside triphosphates were increased and reached their maxima at 10 h after infection. The maximal amount of triphosphorylated drug in a virus producer cell, P3HR-1 (LS), was obtained at 21 h after drug treatment. During long-term drug treatment, approximately 44 and 77% reduction in EBV genome copies per cell was observed on days 3 and 7, respectively. In a separate experiment, after treatment of P3HR-1 (LS) cells with BW B759U for 36 h, 4.2 pmol of BW B759U triphosphate per 10(6) cells was achieved. After the cells were released into drug-free medium, drug triphosphate was rapidly decreased to 11% of the original level in 1 day. Thereafter, the decrease was slow but steady, down to 0.22 pmol/10(6) P3HR-1 cells by 5 days. We calculated that 0.22 pmol of BW B759U triphosphate per 10(6) cells represents a cellular concentration of 0.22 microM, which is theoretically enough to inhibit EBV replication. This is based upon a comparison with the 50% effective dose of BW B759U (0.05 microM) for inhibition of genome replication and a Ki of 0.08 microM for BW B759U triphosphate inhibition of EBV DNA polymerase.
Murine cytomegalovirus infection of mouse testes.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1986

description: A l'aide de la cytohybridation in situ, on a pu mettre en évidence du DNA spécifique du cytomégalovirus murin (CMV) associé aux spermatocytes et au sperme mature après infection des testicules de souris immunocompétentes.
Sequential detection of different antigens induced by Epstein-Barr virus and herpes simplex virus in the same Western blot by using dual antibody probes.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1986

description: A dual antibody probing technique that permitted a color-coded identification of polypeptides representing different classes of Epstein-Barr virus (EBV) antigens as well as differentiation of the polypeptides induced by different herpesviruses in the same Western blot was developed. When the nitrocellulose sheet was probed first with monoclonal antibody against EBV early antigen diffuse component (EA-D) and then stained with 4-chloro-1-naphthol, four polypeptides specific for EA-D were identified by purple bands. Subsequently, the same nitrocellulose sheet was reprobed with human serum containing antibodies against EBV early antigen, viral capsid antigen, and nuclear antigen and stained with 3,3'-diaminobenzidine. Several brown bands corresponding to early, viral capsid, and nuclear antigen polypeptides were detected. The dual antibody probing technique was used in an analysis to differentiate polypeptides resulting from either EBV or herpes simplex virus infection, either in cells infected by individual virus or in a cell line dually infected by both viruses. On the basis of different colored bands in different lanes of the same gel, 20 polypeptides with molecular weights ranging from 31,000 to 165,000 were identified as herpes simplex virus-specific proteins. These results suggested that the dual antibody probing technique may be applicable in clinical diagnosis for detecting antigens and antibodies derived from different pathogens.
The phase transitions of the planar random-cluster and Potts models with q larger than 1 are sharp
Creators: name: Duminil-Copin, Hugo
name: Manolescu, Ioan
Publisher: name: arXiv
Year: 2014
Subjects: subject: Probability (math.PR)
subject: FOS: Mathematics
subject: 60K35, 82B20, 82B26

description: We prove that random-cluster models with q larger than 1 on a variety of planar lattices have a sharp phase transition, that is that there exists some parameter p_c below which the model exhibits exponential decay and above which there exists a.s. an infinite cluster. The result may be extended to the Potts model via the Edwards-Sokal coupling. Our method is based on sharp threshold techniques and certain symmetries of the lattice; in particular it makes no use of self-duality. Part of the argument is not restricted to planar models and may be of some interest for the understanding of random-cluster and Potts models in higher dimensions. Due to its nature, this strategy could be useful in studying other planar models satisfying the FKG lattice condition and some additional differential inequalities.
description: 23 pages, 4 figures
Scene Image is Non-Mutually Exclusive - A Fuzzy Qualitative Scene Understanding
Creators: name: Lim, Chern Hong
name: Risnumawan, Anhar
name: Chan, Chee Seng
Publisher: name: arXiv
Year: 2014
Subjects: subject: Computer Vision and Pattern Recognition (cs.CV)
subject: Artificial Intelligence (cs.AI)
subject: Information Retrieval (cs.IR)
subject: FOS: Computer and information sciences

description: Ambiguity or uncertainty is a pervasive element of many real world decision making processes. Variation in decisions is a norm in this situation when the same problem is posed to different subjects. Psychological and metaphysical research had proven that decision making by human is subjective. It is influenced by many factors such as experience, age, background, etc. Scene understanding is one of the computer vision problems that fall into this category. Conventional methods relax this problem by assuming scene images are mutually exclusive; and therefore, focus on developing different approaches to perform the binary classification tasks. In this paper, we show that scene images are non-mutually exclusive, and propose the Fuzzy Qualitative Rank Classifier (FQRC) to tackle the aforementioned problems. The proposed FQRC provides a ranking interpretation instead of binary decision. Evaluations in term of qualitative and quantitative using large numbers and challenging public scene datasets have shown the effectiveness of our proposed method in modeling the non-mutually exclusive scene images.
description: Accepted in IEEE Transactions on Fuzzy Systems
Vesicular stomatitis virus N and NS proteins form multiple complexes.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1986

description: The vesicular stomatitis virus nucleocapsid protein, N, associated specifically with the viral phosphoprotein, NS, in an in vitro system which supported vesicular stomatitis virus RNA replication. Essentially all the N protein was found complexed with NS. In addition, multiple forms of the N-NS complex were detected which differed in their sedimentation properties and ratios of N to NS.
Eredità e memoria di paesi abbandonati. Documentazione e analisi di centri scomparsi
Creators: name: Damone, Giuseppe
Publisher: name: Universita degli studi di Salerno
Year: 2016
Subjects: subject: ICAR/18 STORIA DELL'ARCHITETTURA
subject: Documentazione
subject: Analisi
subject: Patrimoni

description: 2013 - 2014
description: The issue of abandoned villages and towns is nowadays an important issue around which are articulated complex search paths that cut across several disciplines: from history of architecture and survey, from anthropology to archeology... [edited by Author]