Additional file 1 of IRF8 deficiency-induced myeloid-derived suppressor cell promote immune evasion in lung adenocarcinoma
Creators: name: Gao, Zhen
name: Liu, Shang
name: Xiao, Han
name: Li, Meng
name: Ren, Wan-gang
name: Xu, Lin
name: Peng, Zhong-min
Publisher: name: figshare
Year: 2024
Subjects: subject: Immunology
subject: FOS: Clinical medicine

description: Additional file 1 Figure S1. Identification of immune escape factors in lung adenocarcinoma patients. A-F: Forest plot of survival analysis of the main factors related to immune escape in the 5 lung adenocarcinoma cohorts. Exclusion: T-cell exclusion; CAFs: Cancer-associated fibroblasts, MDSCs: Myeloid-derived suppressor cells; M2: M2 subtype of tumor-associated macrophages; Dysfunction: T-cell dysfunction. F: Venn diagram showing the immune escape factors with a significant impact on survival in the 5 cohorts. Figure S2. Survival analysis between groups with high and low MDSC infiltration in lung adenocarcinoma. A-C: Kaplan‒Meier survival curves between the high- and low-invasive groups in the GSE42127, GSE30219, and GSE14814 cohorts; the log-rank test was used to test the significance of survival rates between groups. D-F: Multivariate Cox regression analysis of the GSE42127, GSE30219, and GSE14814 lung adenocarcinoma cohorts. Mu-Cox multivariate Cox regression analysis; HR: hazard ratio; 95% CI: 95% confidence interval. Figure S3. Assessment of antitumour immune responses between MDSCs with high and low infiltration in the TCGA cohort. A: Correlation heatmap of circular lines between MDSCs and other immune cell infiltration levels in lung adenocarcinoma. B: Ridge plot of differences in the biological behavior of tumor cells between MDSCs with high and low infiltration. C: Violin plot of the differences in antitumour immune responses between MDSCs with high and low infiltration. D: Box plot of the differences in the infiltration levels of immune cells between MDSCs with high and low infiltration. -, no significant difference; *, P &lt; 0.05; **, P &lt; 0.01; ***, P &lt; 0.001; ****, P &lt; 0.0001. Figure S4. Assessment of antitumour immune responses between MDSCs with high and low infiltration in the GSE42127 cohort; A: Correlation heatmap of circular lines between MDSCs and other immune cell infiltration levels in lung adenocarcinoma. B: Violin plot of differences in the biological behavior of tumor cells between MDSCs with high and low infiltration. C: Violin plot of the differences in antitumour immune responses between MDSCs with high and low infiltration. D: Box plot of the differences in the infiltration levels of immune cells between MDSCs with high and low infiltration. -, no significant difference; *, P &lt; 0.05; **, P &lt; 0.01; ***, P &lt; 0.001; ****, P &lt; 0.0001. Figure S5. Assessment of antitumour immune responses between MDSCs with high and low infiltration in the GSE30219 cohort; A: Correlation heatmap of circular lines between MDSCs and other immune cell infiltration levels in lung adenocarcinoma. B: Violin plot of differences in the biological behavior of tumor cells between MDSCs with high and low infiltration. C: Violin plot of the differences in antitumour immune responses between MDSCs with high and low infiltration. D: Box plot of the differences in the infiltration levels of immune cells between MDSCs with high and low infiltration. -, no significant difference; *, P &lt; 0.05; **, P &lt; 0.01; ***, P &lt; 0.001; ****, P &lt; 0.0001. Figure S6. Assessment of antitumour immune responses between MDSCs with high and low infiltration in the GSE14814 cohort; A: Correlation heatmap of circular lines between MDSCs and other immune cell infiltration levels in lung adenocarcinoma. B: Violin plot of differences in the biological behavior of tumor cells between MDSCs with high and low infiltration. C: Violin plot of the differences in antitumour immune responses between MDSCs with high and low infiltration. D: Box plot of the differences in the infiltration levels of immune cells between MDSCs with high and low infiltration. -, no significant difference; *, P &lt; 0.05; **, P &lt; 0.01; ***, P &lt; 0.001; ****, P &lt; 0.0001. Figure S7. Single-cell RNA-Seq analysis of lung adenocarcinoma; A: UMAP after data filtering and quality control. B: Mean expression and percentage expression of selected marker genes in cell types. C: Violin plot of IRF8, EVI2B, RASSF2, and CD4 expression in the main cell subsets. D: Trajectories of EVI2B, RASSF2, and CD4 distribution in myeloid cell subsets. E: Map of EVI2B, RASSF2, and CD4 distribution in myeloid cell subsets. F: Trajectories of EVI2B, RASSF2, and CD4 distribution in MDSC populations. G: Map of EVI2B, RASSF2, and CD4 distribution in MDSC populations. H: Distribution of EVI2B, RASSF2, and CD4 trajectories in myeloid dendritic cell populations. I: Map of EVI2B, RASSF2, and CD4 distribution in myeloid dendritic cell populations. Figure S8. Single-cell RNA-Seq Analysis of Lung Adenocarcinoma; A: Temporal trajectories of macrophages and distribution of IRF8, EVI2B, RASSF2, and CD4. B: Temporal trajectories of monocytes and distribution of IRF8, EVI2B, RASSF2, and CD4. Figure S9. Identification of signalling pathways regulating the differentiation of MDSCs A: GSEA between groups with high and low MDSC infiltration and groups with high and low IRF8 expression in the GSE42127 cohort; B: GSEA between groups with high and low MDSC infiltration and groups with high and low IRF8 expression in the GSE30219 cohort; C: GSEA between groups with high and low MDSC infiltration and groups with high and low IRF8 expression in the GSE14814 cohort.