Additional file 1 of Vancomycin-conjugated polydopamine-coated magnetic nanoparticles for molecular diagnostics of Gram-positive bacteria in whole blood
Creators: name: Abafogi, Abdurhaman Teyib
name: Wu, Tepeng
name: Lee, Daekyu
name: Lee, Jinyeop
name: Cho, Gyoujin
name: Lee, Luke P.
name: Park, Sungsu
Publisher: name: figshare
Year: 2022
Subjects: subject: Space Science
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Genetics
subject: Molecular Biology
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Cancer
subject: Inorganic Chemistry
subject: 110309 Infectious Diseases
subject: FOS: Health sciences

description: Additional file 1. Table S1. Elemental composition of dried van- SiO2-MNPs and their relative atomic concentration were studied by exciting the van- SiO2-MNPs to mono-energetic Al kα x-rays and measuring the energy of photoelectrons emitted by electron energy analyser (ESCALAB250 XPS analyser). Fig. S1. Schematic representing the synthesis of vancomycin-conjugated silica oxide-coated magnetic nanoparticles (van-SiO2-MNPs). Freshly prepared MNPs form larger MNPs through SiO2 coating. SiO2-MNPs are conjugated with van after amino group functionalization [1]. Tetraethyl orthosilicate (TEOS; Sigma-Aldrich). 3-Aminopropyltriethoxysilane (APTES; Sigma-Aldrich). www.biorender.com was used to make the schematics. Fig. S2. (a) Transmission electron microscopy (TEM) images and (b) energy dispersive spectroscopy (EDS) mapping of van-SiO2-MNPs. Morphology and elemental mapping of the particles were obtained at an accelerating voltage of 200kv by using JEM-2100F TEM (JEOL Ltd., Tokyo, Japan) and EDS attached to the TEM machine. Fig. S3. Zeta potential of MNPs, SiO2-MNPs and van-SiO2-MNPs. The potential was measured using Zetasizer Nano ZS (Malvern Instruments, Malvern, UK). Student’s t-test. **: P &lt; 0.01. *: P &lt; 0.05. n = 3. Fig. S4. Microscopic images of van- SiO2-MNPs and van-PDA-MNPs in blood. The images were taken using a DeltaVision microscope (GE Healthcare, Chicago, IL, USA). Before taking the images, blood was mixed with each type of MNPs at 1011 particles/mL (final conc.) and incubated in a rotary shaker for 30 min at RT. Fig. S5. Effect of van-PDA-MNPs and van- SiO2-MNPs on bacteria growth. Each strain was overnight cultured in LB broth with aeration (200 rpm) at 37 °C. Then, the culture was 100 times diluted with fresh LB broth containing either van-PDA-MNPs or van- SiO2-MNPs at 1011 particles/mL (final conc.). The diluted culture was incubated at the same growth condition and its optical density was measured at 600 nm. Fig. S6. Elemental composition of dried van- SiO2-MNPs and their relative atomic concentration were studied by exciting the van- SiO2-MNPs to mono-energetic Al kα x-rays and measuring the energy of photoelectrons emitted by electron energy analyser (ESCALAB250 XPS analyser). Fig. S7. (a) qPCR and (b) the standard curve (Ct value vs bacterial concentration) of S. aureus at different concentrations (101-104 CFU/mL) in PBS preconcentrated by van- SiO2-MNPs and van-PDA-MNPs. Forward (5'-ACACCTGAAACAAAGCATCC-3') and reverse (5'-TAGCCAAGCCTTGACGAACT-3') primers were used to amplify a 207-bp of nuc gene from S. aureus [2]. Fig. S8. Multiplex capturing efficiency of van-PDA-MNPs and van- SiO2-MNPs for different bacterial strains at 105 CFU/mL in PBS (a) and blood (b). The capture efficiency was calculated based on the initial number of bacteria in the sample and the number of uncaptured bacteria cells in the eluent sample. Bacterial numbers were counted using the standard colony counting method. Student’s t-test, *: P &lt; 0.05. NS: P &gt; 0.05. n = 3. Fig. S9. qPCR of different concentrations (101-104 CFU/mL) of S. aureus preconcentrated using van- SiO2-MNPs (a) and van-PDA-MNPs (b) in blood. Fig. S10. qPCR of different concentrations (101-104 CFU/mL) of three bacterial strains (S. aureus (a), MRSA (b) and B. cereus (c)) preconcentrated using van-PDA-MNPs in blood.