Additional file 1 of Hepatic lipid profile in mice fed a choline-deficient, low-methionine diet resembles human non-alcoholic fatty liver disease
Creators: name: Haberl, Elisabeth M.
name: Pohl, Rebekka
name: Rein-Fischboeck, Lisa
name: Höring, Marcus
name: Krautbauer, Sabrina
name: Liebisch, Gerhard
name: Buechler, Christa
Publisher: name: figshare
Year: 2020
Subjects: subject: Biochemistry
subject: Genetics
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Physiology
subject: Biotechnology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: FOS: Chemical sciences
subject: Cancer
subject: Computational Biology

description: Additional file 1: Table S1. Hepatic proteins in mice fed a control or low-methionine choline-deficient (LMCD) diet, and injected either with water (W) or diethylnitrosamine (DEN) (n = 5–11 mice per group). GAPDH was used for normalization except for MnSOD, which was normalized to the Coomassie Blue stained protein levels. Median, minimum (Min) and maximum (Max) values are listed. The P values for comparison of water injected mice (Pwater) and DEN injected mice (PDEN) fed the control chow or LMCD diet are listed. Not significant, ns. Table S2. Levels of triacylglycerol (TG) species are given in nmol/mg. Median value, minimum (Min) and maximum (Max) are listed. The P values for comparison of water (PWater) and DEN injected mice (PDEN) fed the control chow or LMCD diet are listed. Comparison of the mouse groups fed the LMCD diet identified two differentially abundant TG species (PLMCD). Comparison of the mouse groups fed the control chow did not identify any differences (data not shown). Not significant, ns. Table S3. Proportion of triacylglycerol (TG) species in % of total TG. Median value, minimum (Min) and maximum (Max) of % TG are given. The P values for comparison of water injected mice and DEN injected mice fed the control chow are listed. Comparison of water injected mice and DEN injected mice fed the LMCD diet did not identify significant differences. The arrow indicates whether the respective TG is higher or lower in the liver of the DEN injected mouse. Table S4. Levels of free cholesterol (FC), cholesteryl ester (CE) and total cholesterol are given in nmol/mg. Median value, minimum (Min) and maximum (Max) are listed. The P values for comparison of water injected mice (PWater) and DEN injected mice (PDEN) fed the control chow or LMCD diet are listed. Comparison of the mice groups fed the control diet (PControl diet) or LMCD diet (PLMCD) is also given. Not significant, ns. Table S5. Levels of diacylglycerol (DG), phosphatidylcholine (PC), phosphatidylethanolamine (PE), lysophosphatidylcholine (LPC), phosphatidylserine (PS) and phosphatidylinositol (PI) are given in nmol/mg. Median value, minimum (Min) and maximum (Max) are listed. The P values for comparison of water injected mice (PWater) and DEN injected mice (PDEN) fed the control chow or LMCD diet are listed. P values for the comparison of the mice groups fed the control diet (PControl diet) is also given. Comparison of water injected mice and DEN injected mice fed the LMCD diet did not identify significant differences. Not significant, ns; Sat, saturated; MU, monounsaturated; PU, polyunsaturated. Table S6. Lipidome of normal liver and tumor tissues of two mice (1, 2). Levels of free cholesterol (FC), cholesteryl ester (CE), phosphatidylcholine (PC), phosphatidylethanolamine (PE), lysophosphatidylcholine (LPC), phosphatidylserine (PS) and phosphatidylinositol (PI) are given in nmol/mg. Median value, minimum (Min) and maximum (Max) are listed. % change in the tumors is given in the final two columns. Phospholipid, PL.