Simultaneous determination of four phytoecdysteroids by LC-MS/MS: application to a comparative pharmacokinetic study in normal and adjuvant arthritis rats after oral administration of <i>C. officinalis</i> Kuan phytoecdysteroids extract
Creators: name: Wang, Haiqiang
name: Yang, Deqiang
name: Jiang, Shuang
name: Ren, Yixuan
name: Wu, Lihong
name: Wang, Zhenyue
name: Kuang, Haixue
name: Wang, Zhibin
Publisher: name: Taylor & Francis
Year: 2023
Subjects: subject: Biophysics
subject: Biochemistry
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Physiology
subject: Biotechnology
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: Immunology
subject: FOS: Clinical medicine

description: <i>C. officinalis</i> Kuan is the dry root of <i>Cyathula officinalis</i> Kuan. Clinically, it is used for fall and flutter injury, rheumatism and arthralgia. Phytoecdysteroids have significant anti-inflammatory effects, and the phytoecdysteroids present in <i>C. officinalis</i> Kuan exhibit potential for treating rheumatoid arthritis. This study first developed a selective, accurate and efficient LC-MS/MS method for 12-day pharmacokinetic studies regarding the simultaneous determination of cyasterone, 25-epi-28-epi-cyasterone, precyasterone and capitasterone from <i>C. officinalis</i> Kuan phytoecdysteroids extract in normal and adjuvant arthritis rats. An Agilent Eclipse Plus C<sub>18</sub> RRHD column (1.8 µm, 50mm × 2.1 mm) with a gradient mobile phase consisting of water (A) and acetonitrile (B) was used for analysis. The mass analysis was performed in an Agilent 6430 QQQ-MS mass spectrometer with positive mode multiple reaction monitoring (MRM). The results indicated that the AUC<sub>0-t</sub> and AUC<sub>0-∞</sub> values of the four phytoecdysteroids in adjuvant arthritis rats were different from those in normal rats on the first day, which could provide a helpful reference for pharmacological and toxicological studies, as well as clinical applications of <i>C. officinalis</i> Kuan in the treatment of rheumatoid arthritis. 1. <i>C. officinalis</i> Kuan is the dry root of <i>Cyathula officinalis</i> Kuan which has been used for the treatment of flapping injury, rheumatism arthralgia, foot flaccidity, and tendon contracture thousands of years in China, and has been officially included in the Chinese Pharmacopoeia. 2. A highly accurate, stable, and sensitive ultra-performance liquid chromatography with tandem mass spectrometry (UHPLC-MS/MS) method was first established and validated for simultaneously determination four phytoecdysteroids: cyasterone, 25-epi-28-epi-cyasterone, precyasterone and capitasterone in normal and adjuvant arthritis rats plasma samples 12 days of continuous gavage of <i>C. officinalis</i> Kuan phytoecdysteroids extract. 3. The phytoecdysteroids is the important component of <i>C. officinalis</i> Kuan, which is difficult to separated. And there is no report for the pharmacokinetic study of phytoecdysteroids from <i>C. officinalis</i> Kuan. And the method provides a good reference for the follow-up studies clinical medication of the phytoecdysteroids from <i>C. officinalis</i> Kuan.