Additional file 1 of Isolation of TSCD11 Gene for Early Chloroplast Development under High Temperature in Rice
Creators: name: Fang, Guonan
name: Yang, Shenglong
name: Ruan, Banpu
name: Liu, Chaolei
name: Zhang, Anpeng
name: Jiang, Hongzhen
name: Ding, Shilin
name: Tian, Biao
name: Zhang, Yu
name: Jahan, Noushin
name: Zhu, Li
name: Zhang, Guangheng
name: Dong, Guojun
name: Zhang, Qiang
name: Zeng, Dali
name: Guo, Longbiao
name: Gao, Zhenyu
name: Qian, Qian
Publisher: name: figshare
Year: 2020
Subjects: subject: Genetics
subject: FOS: Biological sciences
subject: Plant Biology

description: Additional file 1 Fig. S1 Comparison of phenotypes and pigment contents between the wild-type and tscd11 plants. a-d Phenotypes of the WT and tscd11 at the 2-leaf stage grown at continuous 25 °C (a, b) and 35 °C (c, d), respectively. e-h Phenotypes of the WT and tscd11 at the 3-leaf stage grown at continuous 25 °C, shift 25 °C to 35 °C, continuous 35 °C, shift 35 °C to 25 °C, respectively. Bar = 2 cm. i-l Pigment contents of the new third leaf of the wild-type and tscd11 plants grown at continuous 25 °C, shift 25 °C to 35 °C, continuous 35 °C, shift 35 °C to 25 °C at the 3-leaf stage. Data represent mean ± SD (n = 3). * p &lt; 0.05, ** p &lt; 0.01 (Student’s t-test). Fig. S2 Phenotypes and pigment contents comparison between wild-type and tscd11 plants. a, d Phenotypes of the WT and tscd11 at the tillering (bar = 12 cm), and heading stage (bar = 10 cm), respectively, in the paddy field. b, e Close-up image of wild-type leaf and tscd11 leaf at the tillering, and heading stage (bar = 5 cm), respectively. c, f Pigment contents of WT and tscd11 at the tillering, and heading stage, respectively. Data represent mean ± SD (n = 3). * p &lt; 0.05, ** p &lt; 0.01 (Student’s t-test). Fig. S3 ROS accumulation and genomic DNA fragmentation in the tscd11 mutant. a, b, f, g DAB (a, f) and NBT (b, g) staining of leaves from wild-type and tscd11 plants at 25 °C (a, b) and 35 °C (f, g), respectively (bar =1 cm). c-e, h-j Statistic analysis of H2O2 content (c, h), MDA content (d, i), CAT activity (e, j) at 25 °C (c-e) and 35 °C (h-j), respectively. k, l Relative expression levels of senescence, ROS-responsive and ROS detoxification related genes in wild-type and tscd11 plants at 25 °C (k) and 35 °C (l) The rice Histone gene was used as an internal control. The expression level of each tested genes in WT was set to 1.0. Data represent mean ± SD (n = 3). * p &lt; 0.05, ** p &lt; 0.01 (Student’s t-test). Fig. S4 TUNEL assay of wild-type and tscd11 leaves at 25 °C and 35 °C. Red signal is PI staining, green color represents positive result of apoptotic cells (bar = 50 μm). a, b leaves from wild-type and tscd11 plants at 25 °C. c, d leaves from wild-type and tscd11 plants at 35 °C. Fig. S5 Transcriptional expression of chloroplast-encoded genes. a, Expression of chloroplast-encoded genes in the WT and tscd11 mutant at 25 °C. b, Expression of chloroplast-encoded genes in the WT and tscd11 mutant at 35 °C. The rice Histone gene was used as an internal control. The expression level of each tested gene in the WT was set to 1.0. Data represent mean ± SD (n = 3). * p &lt; 0.05, ** p &lt; 0.01 (Student’s t-test).