cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Synthesis, crystal structure and antifungal activity of a newly synthesized polymeric mixed ligand complex of Zn(II) with 1,1-dithiolate and nitrogen donors
Creators: name: Singh, Mahesh Kumar
name: Sanjit Sutradhar
name: Bijaya Paul
name: Adhikari, Suman
name: Butcher, Raymond J.
name: Acharya, Sandeep
name: Arijit Das
Publisher: name: Taylor & Francis
Year: 2014
Subjects: subject: Chemistry
subject: Biotechnology
subject: Cell Biology
subject: Microbiology
subject: FOS: Biological sciences
subject: Biochemistry
subject: Biophysics

description: A new mixed ligand complex of Zn(II) with 1,1-dicyanoethylene-2,2-dithiolate [i-MNT2- = {S2C:C(CN)2}2-] and 1,3-diaminopropane as ligands has been synthesized and characterized on the basis of spectroscopy and single-crystal X-ray diffraction analysis. Spectroscopic studies and single-crystal X-ray diffraction analysis reveal that the zinc(II) complex is tetrahedral and adopts a 1,3-diaminopropane assisted linear polymeric assembly. The molar conductance data of the complex in DMF solution show non-electrolytic nature. Biological screening effects in vitro of the complex have been tested against five fungi Synchitrium endobioticum, Pyricularia oryzae, Helminthosporium oryzae, Candida albicans (ATCC10231), Trichophyton mentagrophytes by the disc diffusion method. A comparative study of inhibition zone values of the primary ligand K2i-MNT·H2O and its synthesized complex indicates that the complex exhibits fungistatic antifungal activity whereas K2i-MNT·H2O does not affect Pyricularia oryzae.
Mechanistic Regulation of Planarian Shape During Growth and Degrowth
Creators: name: Ko, Jason M.
name: Reginato, Waverly
name: Lobo, Daniel
Publisher: name: Maryland Shared Open Access Repository
Year: 2023

description: Adult planarians can grow when fed and degrow (shrink) when starved while maintaining their whole-body shape with correct proportions. Different planarian morphogens are expressed at the anterior-posterior poles, medio-lateral border, and midline to provide positional information signals for the specification of different tissues at the right locations. However, it is currently unknown how these signals are coordinated together during feeding or starvation and how they modulate the differential tissue growth or degrowth necessary to form correct whole-body shapes. Here we investigate the dynamics of planarian shape during growth and degrowth together with a theoretical study to evaluate the mechanisms that regulate whole-body proportions and shape. We found that the planarian body proportions scale isometrically following similar linear rates during growth and degrowth, but that fed worms are significantly wider than starved worms. By combining a descriptive model of planarian shape and size with a mechanistic model of anterior-posterior and medio-lateral signaling calibrated with a novel machine learning methodology, we demonstrate that the feedback loop between these positional information signals and the shape they control can regulate the planarian whole-body shape during growth. Furthermore, the model can predict the correct shape and size dynamics during degrowth due to an increase in apoptosis rate and pole signal during starvation. These results offer mechanistic insights into planarian shape and size dynamics and the regulation of whole-body morphologies.
title: Les sports collectifs : l'émergence des émotions des élèves dans les activités coopératives, compétitives et coopétitives
title: Les sports collectifs : l'émergence des émotions des élèves dans les activités coopératives, compétitives et coopétitives
titleType: AlternativeTitle
Creators: name: Frutschi, Miriam
name: Romanens, Jonathan
Publisher: name: Unknown
Year: 0
Subjects: subject: compétition
subject: coopération
subject: coopétition
subject: émotions
subject: éducation physique et sportive
subject: football

description: Dans le cadre de notre recherche, nous nous intéressons aux émotions des élèves lors des leçons d’éducation physique et sportive. Nous avons choisi d’observer quatre élèves dans le cadre des jeux collectifs, plus précisément dans le cadre du football. Nous avons pour cela sélectionné trois approches : la coopération, la coopétition et la compétition. Dans le cadre de notre recherche, nous souhaitons savoir dans quelle approche les émotions sont les plus positives chez les élèves. La compétition permet le dépassement de soi et la persévérance mais peut occasionner diverses émotions négatives, comme l’anxiété, la colère ou la tristesse. La coopération, quant à elle, permet de développer diverses capacités transversales chez les élèves, comme l’entraide, la communication ou la pensée réflexive. Dans notre hypothèse, nous estimons que les élèves ont besoin de ces deux approches pour pouvoir rester motivés et avancer dans leurs apprentissages. Nous estimons alors que la coopétition permet d’allier les avantages que l’on retrouve à la fois dans la compétition et à la fois dans la coopération. Nous supposons que cette approche, étant donné qu’elle regroupe la compétition et la coopération, permettra aux élèves de ressentir davantage d’émotions positives que dans les deux autres approches. Notre question de recherche est donc la suivante : « Les activités coopétitives dans les jeux permettent-elles un meilleur ressenti émotionnel chez les élèves comparativement aux activités compétitives et coopératives ? ». Pour répondre à notre hypothèse et à notre question de recherche, nous avons mené trois leçons dans une classe de 7ème Harmos. Une leçon était basée sur la coopération, une autre sur la coopétition et une troisième sur la compétition. Nous avons choisi d’observer quatre élèves dans le cadre du football. Pour cela, nous avons choisi deux footballeurs et deux nonfootballeurs et avons comptabilisé le nombre d’émotions positives et négatives ressenties par les élèves au cours de la séquence. Nous avons pour finir vérifié dans quelle séance les émotions étaient les plus positives. Après chaque leçon, les élèves ont répondu à un questionnaire et nous avons à chaque fois interviewé deux élèves, un footballeur et un non-footballeur. A la fin de notre recherche, notre hypothèse de base n’a pas été confirmée. En effet, nous estimions que les émotions des élèves seraient susceptibles d’être les plus positives lors des activités coopétitives, ce qui n’a pas été le cas. La coopération a été l’approche la plus appréciée des élèves et c’est là où les émotions ont été les plus positives.
A unified framework for the analysis of accuracy and stability of a class of approximate Gaussian filters for the Navier-Stokes Equations
Creators: name: Biswas, Animikh
name: Branicki, Michal
Publisher: name: Maryland Shared Open Access Repository
Year: 2024
Subjects: Mathematics - Analysis of PDEs
Mathematics - Optimization and Control
Mathematics - Probability

description: Bayesian state estimation of a dynamical system utilising a stream of noisy measurements is important in many geophysical and engineering applications. We establish rigorous results on (time-asymptotic) accuracy and stability of these algorithms with general covariance and observation operators. The accuracy and stability results for EnKF and EnSRKF for dissipative PDEs are, to the best of our knowledge, completely new in this general setting. It turns out that a hitherto unexploited cancellation property involving the ensemble covariance and observation operators and the concept of covariance localization in conjunction with covariance inflation play a pivotal role in the accuracy and stability for EnKF and EnSRKF. Our approach also elucidates the links, via determining functionals, between the approximate-Bayesian and control-theoretic approaches to data assimilation. We consider the `model' dynamics governed by the two-dimensional incompressible Navier-Stokes equations and observations given by noisy measurements of averaged volume elements or spectral/modal observations of the velocity field. In this setup, several continuous-time data assimilation techniques, namely the so-called 3DVar, EnKF and EnSRKF reduce to a stochastically forced Navier-Stokes equations. For the first time, we derive conditions for accuracy and stability of EnKF and EnSRKF. The derived bounds are given for the limit supremum of the expected value of the $L^2$ norm and of the $\mathbb{H}^1$ Sobolev norm of the difference between the approximating solution and the actual solution as the time tends to infinity. Moreover, our analysis reveals an interplay between the resolution of the observations associated with the observation operator underlying the data assimilation algorithms and covariance inflation and localization which are employed in practice for improved filter performance.
Engineering whiting events in culture: A microalgae-driven calcium carbonate and biomass production process at high pH and alkalinity with the marine microalga Nannochloropsis oceanica IMET1
Creators: name: Lee, Yi-Ying
name: Jonas, Lauren
name: Hill, Russell
name: Place, Allen
name: Silsbe, Greg
name: Hunsicker, Scott
name: North, Elizabeth
name: Li, Yantao
Publisher: name: Elsevier
Year: 2024
Subjects: Carbon capture and storage
Microbiologically induced calcium carbonate precipitation (MICP)
Monohydrocalcite
Photobioreactor
Whiting

description: The impact of whiting events on global inorganic carbon cycle and climate change is debatable at the current ocean pH. This work engineered whiting events in a high pH, high alkalinity microalgal culture for carbon dioxide capture and storage. When growing the marine microalga Nannochloropsis oceanica IMET1 in photobioreactors, culture alkalinity more than doubled, increasing from 72.5 mg L-1 to a maximum level of 159.6 mg L-1. At the same time, culture pH increased from 7.9 to 10.1 with concomitant calcium carbonate production. X-ray diffraction analysis revealed the precipitated calcium carbonate was primarily monohydrocalcite. The lab culture was scaled up to a 340-L bioreactor, in which Nannochloropsis ash-free dry weight productivities ranged from 25.1 to 51.4 g m-2 d-1 and a maximum monohydrocalcite productivity of 133.4 g m2 d-1 was recorded. In this system, Nannochloropsis biomass contained about 23.9 % lipids and the eicosapentaenoic acid content was about 1.8 %. Together, these results suggest the microalgae-driven calcium carbonate and biomass production process efficiently captures and stores atmospheric carbon dioxide in the form of calcium carbonate while producing valuable bioproducts. Study of the bacterial communities associated with the Nannochloropsis culture identified four dominant species Maricaulis maris, Marinisubtilis pacificus, Gracilimonas sp., and an uncultured bacterium in the OD1 phylum with interesting features that warrant further investigation.
GC–MS characterisation and biological activity of essential oils from different vegetative organs of Plectranthus barbatus and Plectranthus caninus cultivated in north Italy
Creators: name: F. Gelmini
name: P. Squillace
name: C. Testa
name: A.C. Sparacino
name: S. Angioletti
name: G. Beretta
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Developmental Biology
subject: Biological Sciences
subject: Meta Science
subject: Ecology
subject: FOS: Biological sciences
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Microbiology
subject: Biochemistry

description: Essential oils (EOs) from the roots, stems and leaves of Plectranthus barbatus (A) and Plectranthuscaninus (B), cultivated in north Italy, were obtained by steam distillation and chemically characterised by gas chromatography–mass spectrometry. The highest yields were obtained from roots (268.15 and 673.60 mg/kg from A and B), followed by leaves (64.34 and 26.65 mg/kg) and stems (19.76 and 18.63 mg/kg). A total of 128 structures were identified in A and 121 in B. Fe++ chelating and antiradical activities (DPPH and ABTS) were evaluated: root and stem EOs showed the strongest activities, while EOs from leaves did not show relevant activities. All EOs were tested for their in vitro antimicrobial activity, showing optimal growth-inhibition in antibiogram (∅>35 mm) and MIC tests (32–64 μg/mL) against Candida albicans, while EOs from leaves of both species showed a good activity (25 Escherichia coli.
Genetic compensation of triacylglycerol biosynthesis in the green microalga Chlamydomonas reinhardtii
Creators: name: Lee, Yi-Ying
name: Park, Rudolph
name: Miller, Stephen M.
name: Li, Yantao
Publisher: name: Wiley
Year: 2022
Subjects: Chlamydomonas reinhardtii
CRISPR
gene knockout
genetic compensation
triacylglycerol biosynthesis

description: Genetic compensation has been proposed to explain phenotypic differences between gene knockouts and knockdowns in several metazoan and plant model systems. With the rapid development of reverse genetic tools such as CRISPR/Cas9 and RNAi in microalgae, it is increasingly important to assess whether genetic compensation affects the phenotype of engineered algal mutants. While exploring triacylglycerol (TAG) biosynthesis pathways in the model alga Chlamydomonas reinhardtii, it was discovered that knockout of certain genes catalyzing rate-limiting steps of TAG biosynthesis, type-2 diacylglycerol acyltransferase genes (DGTTs), triggered genetic compensation under abiotic stress conditions. Genetic compensation of a DGTT1 null mutation by a related PDAT gene was observed regardless of the strain background or mutagenesis approach, for example, CRISPR/Cas 9 or insertional mutagenesis. However, no compensation was found in the PDAT knockout mutant. The effect of PDAT knockout was evaluated in a ?vtc1 mutant, in which PDAT was upregulated under stress, resulting in a 90% increase in TAG content. Knockout of PDAT in the ?vtc1 background induced a 12.8-fold upregulation of DGTT1 and a 272.3% increase in TAG content in ?vtc1/pdat1 cells, while remaining viable. These data suggest that genetic compensation contributes to the genetic robustness of microalgal TAG biosynthetic pathways, maintaining lipid and redox homeostasis in the knockout mutants under abiotic stress. This work demonstrates examples of genetic compensation in microalgae, implies the physiological relevance of genetic compensation in TAG biosynthesis under stress, and provides guidance for future genetic engineering and mutant characterization efforts.
PDAT regulates PE as transient carbon sink alternative to triacylglycerol in Nannochloropsis
Creators: name: Yang, Juan
name: Liu, Jin
name: Pan, Yufang
name: Maréchal, Eric
name: Amato, Alberto
name: Liu, Meijing
name: Gong, Yangmin
name: Li, Yantao
name: Hu, Hanhua
Publisher: name: Oxford University Press
Year: 2022

description: Triacylglycerols (TAGs) are the main storage lipids in photosynthetic organisms under stress. In the oleaginous alga Nannochloropsis oceanica, while multiple acyl CoA:diacylglycerol (DAG) acyltransferases (NoDGATs) are involved in TAG production, the role of the unique phospholipid:DAG acyltransferase (NoPDAT) remains unknown. Here, we performed a functional complementation assay in TAG-deficient yeast (Saccharomyces cerevisiae) and an in vitro assay to probe the acyltransferase activity of NoPDAT. Subcellular localization, overexpression, and knockdown (KD) experiments were also conducted to elucidate the role of NoPDAT in N. oceanica. NoPDAT, residing at the outermost plastid membrane, does not phylogenetically fall into the clades of algae or plants and uses phosphatidylethanolamine (PE) and phosphatidylglycerol with 16:0, 16:1, and 18:1 at position sn-2 as acyl-donors in vivo. NoPDAT KD, not triggering any compensatory mechanism via DGATs, led to an ?30% decrease of TAG content, accompanied by a vast accumulation of PEs rich in 16:0, 16:1, and 18:1 fatty acids (referred to as “LU-PE”) that was positively associated with CO2 availability. We conclude that the NoPDAT pathway is parallel to and independent of the NoDGAT pathway for oil production. LU-PE can serve as an alternative carbon sink for photosynthetically assimilated carbon in N. oceanica when PDAT-mediated TAG biosynthesis is compromised or under stress in the presence of high CO2 levels.
Microalgal and bacterial auxin biosynthesis: implications for algal biotechnology
Creators: name: Lin, Hanzhi
name: Li, Yantao
name: Hill, Russell T.
Publisher: name: Elsevier
Year: 2021

description: Optimization of microalgal growth and high-value metabolite production are key steps in microalgal mass culture for the algae industry. An emerging technology is the use of phytohormones, like indole-3-acetic acid (IAA), to promote microalgal growth. This requires an understanding of the biosynthesis of IAA in microalgae-bacteria associations and its function in regulating algal physiology and metabolite production. We review the current advances in understanding of microalgal and bacterial auxin biosynthesis and their implications for algal biotechnology.
Hvordan kan geografiske informasjonssystemer gjøre trafikkingeniørens hverdag enklere?
Creators: name: Wiggen, Frode
Publisher: name: Proceedings from the Annual Transport Conference at Aalborg University
Year: 2020

description: Beslutningstakerne i dagens samfunn, i form av ledere og politikere, er ikke i like stor grad som før fagpersoner. For at disse beslutningstakerne skal kunne ta avgjørelser basert på et best mulig faglig grunnlag, er man avhengig av å presentere det faglige grunnlaget på en enkel og forståelig måte. Dette har ført til et stort behov for å kunne presentere data og resultater visuelt og i form av kartpresentasjoner. Slike visuelle presentasjoner gir god oversikt og er enkle å forstå. Dette gjør at selv om man ikke er ekspert på fagområdet, kan man enkelt og raskt oppfatte og absorbere mye og tildels komplisert informasjon. Som trafikkingeniør og fagperson kan man også dra stor nytte av det å kunne visualisere fagdata. Gjennom noen eksempler vises det hvordan geografiske informasjonssystemer (GIS) effektivt brukes til å analysere, presentere og kontrollere ulike fagdata.
description: Proceedings from the Annual Transport Conference at Aalborg University, Vol. 1 No. 1 (2004): Proceedings from the Annual Transport Conference at Aalborg University
In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly
Creators: name: Wolff, Andrew
name: Wagner, Cynthia
name: Wolf, Julia
name: Lobo, Daniel
Publisher: name: Cell Press
Year: 2022
Subjects: Developmental biology
Model Organisms
Molecular Biology

description: The synthesis of single-stranded riboprobes or double-stranded RNAs for in situ hybridization and gene knockdowns often use vectors that require time-consuming plasmid restriction digests and inefficient gel purifications. Here, we present a faster protocol for the simultaneous plasmid restriction digestion and Gibson assembly of vectors for the synthesis of both riboprobes and double-stranded RNAs for in situ and RNA interference experiments, respectively. We illustrate the protocol with planaria in situ and RNAi assays, but it is applicable to any organism.
Combining the body and mobile technology to teach English pronunciation
Creators: name: Zhang, Felicia
Publisher: name: figshare
Year: 2014
Subjects: Language
Education

description: In Australia, postgraduate international student enrolments have risen 81 per cent since 2002. In 2008, 74 per cent of international enrolments were in Business/Management, Computer Science and Engineering-related courses (Davies, 2010). Most international students are from Asia, and the vast majority of those from mainland China. A survey of employers in 2006 found that employers rated interpersonal and communications skills (written, oral, listening) far above “qualifications” and “previous employment” as the most important selection criteria when hiring graduates (Davies, 2010). In fact, research on the IELTS levels of exiting Master’s degree students suggests that international students at Australian universities tended to graduate with worse spoken English skills than when they first entered.
The above research has identified a deficiency in the communication skills of international students, crucial in seeking employment. This chapter proposes various ways of helping committed international students on campus to improve their spoken English language by combining the use of the students’ bodies with mobile technology.
Writing papers using Nvivo and Coh Metrix
Creators: name: Zhang, Felicia
Publisher: name: figshare
Year: 2014
Subjects: Language
Education

description: With the release of NVivo 8 in 2008, this chapter first discusses the use of NVivo 8 to conduct a literature review using the writing of this paper as an example. NVivo 8 has overcome a major challenge to a research project of importing sources in pdf files. It now is also capable of capturing video and audio as secondary data sources. Transcription of sound and video data sources is also available. NVivo 8 has been very useful for analyzing literature to support theory generation. It is also a good management tool for organizing the sources in any research project. The paper will be of interest to novice researchers, as a reference in writing a literature review. In the second part of the paper, after written the chapter with the help of NVivo 8, the use of Coh-Metrix will be used to benchmark the quality of the writing against the author’s previously published papers. Coh-Metrix, is a web-based computer tool that analyzes texts on over 200 measures of cohesion, language, and readability. Standard text readability formulas scale texts on difficulty by relying on word length and sentence length, whereas Coh-Metrix is sensitive to cohesion relations, world knowledge, and language and discourse characteristics. In this chapter, the author would use argument overlap, latent semantic analysis (LSA), and Flesch-Kincaid grade level (Kincaid, Fishburne, Rogers, & Chissom, 1975) to assess readability difficulty in a number of previously published papers in Education and applied linguistics written by the author. This establishes a benchmark for her papers. It is argued that the use of NVivo 8 and Coh-Metrix is capable of increasing the quality of submissions for publication and will be an excellent set of tools for postgraduate students to use for publication purposes.
New Flexible Analogues of 8-Aza-7-deazapurine Nucleosides as Potential Antibacterial Agents
Creators: name: Khandazhinskaya, Anastasia
name: Eletskaya, Barbara
name: Mironov, Anton
name: Konstantinova, Irina
name: Efremenkova, Olga
name: Andreevskaya, Sofya
name: Smirnova, Tatiana
name: Chernousova, Larisa
name: Kondrashova, Evgenia
name: Chizhov, Alexander
name: Seley-Radtke, Katherine
name: Kochetkov, Sergey
name: Matyugina, Elena
Publisher: name: MDPI
Year: 2023
Subjects: antibacterial activity
antituberculosis activity
fleximer
inhibitor
nucleoside analogues

description: A variety of ribo-, 2?-deoxyribo-, and 5?-norcarbocyclic derivatives of the 8-aza-7-deazahypoxanthine fleximer scaffolds were designed, synthesized, and screened for antibacterial activity. Both chemical and chemoenzymatic methods of synthesis for the 8-aza-7-deazainosine fleximers were compared. In the case of the 8-aza-7-deazahypoxanthine fleximer, the transglycosylation reaction proceeded with the formation of side products. In the case of the protected fleximer base, 1-(4-benzyloxypyrimidin-5-yl)pyrazole, the reaction proceeded selectively with formation of only one product. However, both synthetic routes to realize the fleximer ribonucleoside (3) worked with equal efficiency. The new compounds, as well as some 8-aza-7-deazapurine nucleosides synthesized previously, were studied against Gram-positive and Gram-negative bacteria and M. tuberculosis. It was shown that 1-(?-D-ribofuranosyl)-4-(2-aminopyridin-3-yl)pyrazole (19) and 1-(2?,3?,4?-trihydroxycyclopent-1?-yl)-4-(pyrimidin-4(3H)-on-5-yl)pyrazole (9) were able to inhibit the growth of M. smegmatis mc2 155 by 99% at concentrations (MIC99) of 50 and 13 µg/mL, respectively. Antimycobacterial activities were revealed for 4-(4-aminopyridin-3-yl)-1H-pyrazol (10) and 1-(4?-hydroxy-2?-cyclopenten-1?-yl)-4-(4-benzyloxypyrimidin-5-yl)pyrazole (6). At concentrations (MIC99) of 40 and 20 µg/mL, respectively, the compounds resulted in 99% inhibition of M. tuberculosis growth.
Learning Mandarin Chinese Prosody: a Somatically-enhanced Approach for second Language learners
Creators: name: Zhang, Felicia
Publisher: name: figshare
Year: 2014
Subjects: Language
Education

description: This paper reports on an empirical study on the use of active learning techniques, the learning of Mandarin Chinese in a regional university in Australia. The active learning techniques are techniques derived from an active language learning approach known as the 'Somatically-enhanced Approach' (SEA) (Zhang, 2006) to language learning. These included humming, clapping to rhythms of the language, using movement and gestures to enhance the perception of rhythmic patterns in a language. This empirical study was evaluated using a mixture of quantitative and qualitative approaches with the aims of answering one question: Does the active learning techniques benefit student learning from the L2 learners' point of view? If so, in what way do they benefit L2 students? Due to lack of space in this paper, only the quantitative results of the study are reported.
Indoor environmental irritant, dibutyl phthalate (DBP) and sensory irritation in the airway: contribution to asthma symptoms?
Creators: name: Bolaji, James Adewale
Publisher: name: Imperial College London
Year: 2019

description: Epidemiological studies have suggested that environmental exposure to Dibutyl phthalate (DBP) as an indoor air pollutant, found in flooring, cosmetics and food packaging, is linked with worsening or development of asthma. It is thought that due to its high lipophilicity, DBP can accumulate in tissues, including the lung and may contribute to symptoms in this way. Though the potential cellular mechanisms behind DBP’s action are not entirely clear, DBP has been shown to activate some of the Transient Receptor Potential (TRP) family of ion channels, some of which are expressed on airway sensory nerves. Activation of these nerves can lead to respiratory reflexes which are exacerbated in asthma, such as cough and bronchospasm. The aim of this thesis was to investigate whether exposure to DBP can activate airway sensory nerves and identify possible mechanisms behind this. Additionally, this thesis aimed to investigate whether any potential nerve activation would worsen asthma symptoms in an animal model. Using an in vivo model of single afferent airway nerve firing in anaesthetised guinea-pigs, aerosolised administration of DBP caused firing of airway Aδ- and C-fibres. Furthermore, using in vitro nerve depolarisation and calcium imaging assays showed that DBP could activate the whole nerve trunk as well as induce calcium flux in airway terminating jugular and nodose neurons. With the use of pharmacological tools and tissues from genetically modified mice, DBP’s action on nerves/neurons was shown to be mediated through activation of TRPA1, TRPV1 and TRPV4, which are all known to activate sensory nerves and lead to respiratory reflexes such as cough and bronchospasm. Interestingly, in vivo single afferent nerve assays showed that activation of airway C-fibres was through activation of TRPA1 and TRPV1, whereas TRPV4 was responsible for DBP-induced activation of airway Aδ-fibres. Further in vitro investigation using pharmacological tools showed that DBP’s action on TRPA1 was due to mitochondrial-derived oxidative stress, mediated by the aryl hydrocarbon receptor. Additionally, DBP-induced activation of TRPV1 was shown to be mediated through prostanoid products (e.g. PGE2), and DBP-induced activation of TRPV4 involved the subsequent release of ATP. Finally, exposure to DBP in an allergic model of asthma in the rat showed a trend to exacerbated responses in increases in PenH and BALF eosinophils, though this was not significantly different. Data in this thesis have shown that exposure to DBP can activate airway sensory nerves and identified novel mechanisms by which DBP can do this. During this thesis, it was not possible to conclude that exposure to DBP exacerbated a disease model, though further investigation (e.g. in a different model or looking at the effect of DBP on cough) may show how DBP may worsen or trigger asthma symptoms.
Collaboration in Truck Appointment System in Container Terminals
Creators: name: Karam, Ahmed
name: Azab, Ahmed E.
name: Eltawil, Amr B.
name: Reinau, Kristian Hegner
Publisher: name: Proceedings from the Annual Transport Conference at Aalborg University
Year: 2019

description: Due to the continual increase of the global containerized trade, many container terminals face the problem of high demands that their current resource capacity cannot afford. The consequences of such situation are not only the long queues of trucks at the entrance gates and storage yards but also the large turnaround times of trucks. In response, Truck Appointment Systems (TAS) were introduced to schedule truck arrivals in order to alleviate the terminal rush hours, however, the mandatory appointments developed by TASs have a negative impact on the operations as well as resources of the trucking companies. In recent literature, this issue was considered by introducing collaborative TAS in which the trucking companies as well as the container terminals collaborate to set the truck appointments. This work elaborates on the difference between traditional and collaborative TAS and demonstrates how collaborative TAS can improve the performance of the container terminal and reduce the cost of the trucking companies.
description: Proceedings from the Annual Transport Conference at Aalborg University, Vol. 26 No. 1 (2019): Proceedings from the Annual Transport Conference at Aalborg University
Collaborative delivery strategies for goods delivery
Creators: name: Karam, Ahmed
name: Reinau, Kristian Hegner
Publisher: name: Proceedings from the Annual Transport Conference at Aalborg University
Year: 2019

description: Transportation sector has the highest growth rate of greenhouse gas emissions compared to other sectors. The fact that the current practice of freight transportation strategies is not sustainable in the long run, motivates the urgent need of sustainable transportation strategies with less negative effects on the environment and the society. As one of the possible sustainable solutions, collaborative planning of freight delivery has attracted the interest of professional and scientific communities. This work illustrates some strategies of the collaborative freight transportation.
description: Proceedings from the Annual Transport Conference at Aalborg University, Vol. 26 No. 1 (2019): Proceedings from the Annual Transport Conference at Aalborg University
Meeting report: 36th International Conference on Antiviral Research in Lyon, France – March 13–17, 2023
Creators: name: Spengler, Jessica R.
name: Carter, Kara
name: Delang, Leen
name: Durantel, David
name: Gowen, Brian B.
name: Herrero, Lara J.
name: Hurst, Brett
name: Janeba, Zlatko
name: Jordan, Robert
name: Luo, Dahai
name: Meier, Chris
name: Moffat, Jennifer
name: Rocha-Pereira, Joana
name: Seley-Radtke, Katherine
name: Welch, Stephen R.
name: Schang, Luis M.
Publisher: name: Elsevier
Year: 2023

description: The 36th International Conference on Antiviral Research (ICAR), sponsored by the International Society for Antiviral Research (ISAR), was held March 13–17, 2023, in Lyon, France, and concurrently through an interactive remote meeting platform. Here we provide a report summarizing the presentations at the 36th ICAR, including the ISAR speaker awards. We also detail special events, sessions, and additional awards conferred at the meeting. ICAR returned to in-person meetings in 2022, convening in Seattle, WA, USA. The 36th ICAR is the first in-person meeting of the society in Europe since the beginning of the COVID-19 pandemic, which restricted most events to virtual attendance to help mitigate the spread and subsequent public health impact of SARS-CoV-2. An exceptionally high number of registrants and record attendance at this year's ICAR, along with a vast array of demonstrable expertise in a variety of antiviral research-related fields, reflected a strong and growing antiviral research community committed to improving health outcomes from viral diseases, including SARS-CoV-2, and to future pandemic preparedness. This report highlights the breadth of expertise, quality of research, and notable advancements that were contributed by members of ISAR and other participants at the meeting. ICAR aims to continue to provide a platform for sharing information, fostering collaborations, and supporting trainees in the field of antiviral research. The 37th ICAR will be held in Gold Coast, Australia, May 20–24, 2024.
Cosmos Fluid theory - the flyby anomaly
Creators: name: Kitchen, Kris
Publisher: name: figshare
Year: 2014
Subjects: subject: Interstellar and Intergalactic Matter
subject: Instrumentation, Techniques, and Astronomical Observations
subject: 20104 Galactic Astronomy
subject: FOS: Physical sciences
subject: Cosmology
subject: Planetary Geology
subject: Stellar Astronomy
subject: Stars, Variable Stars
subject: Astrophysics
subject: Planetary Science
subject: Solar System, Solar Physics, Planets and Exoplanets
subject: Extragalactic Astronomy
subject: Space Science

description: Cosmos Fluid theory
BILENS ROLL I DEN DAGLIGA RÖRLIGHETEN.
Creators: name: Krantz, Lars-Gunnar
name: Vilhelmson, Bertil
Publisher: name: Proceedings from the Annual Transport Conference at Aalborg University
Year: 2020

description: Bilen är idag det dominerande färdmedlet för den dagliga rörligheten i Sverige, liksom i de flesta andra industrialiserades länder. Denna studie syftar till att analysera utvecklingen av bilinnehav samt bilanvändning i Sverige mellan 1978 och 1994 samt dess fördelning mellan olika grupper t ex åldersgrupper, hushållstyper samt män och kvinnor. Dessutom diskuteras förutsättningarna för den framtida utvecklingen av bilinnehav och bilanvändning och därmed rörligheten. Analysen av bilinnehav och bilanvändning använder uppgifter från de svenska rikstäckande resvaneundersökningar som genomförts 1978 och 1984/85 samt material för år 1994 från den pågående undersökningen (Riks-RVU). Några viktiga iakttagelser är: - Andelen individer med körkort och tillgång till bil i hushållet ökar i Sverige mellan 1978 och 1994. - Körkortsinnehav och tillgång till bil ökar mest bland de äldre och kvinnor medan det minskar i den yngsta åldersgruppen, 18-24 år. - Majoriteten av befolkningen visar tendenser att ha nått en mättnadsnivå av bilinnehav vid en första anblick. Bland de sammanboende ökar dock andelen som har mer än en bil i hushållet. - Bilanvändningen mätt som reslängd per person och dygn har inte ökat i samma takt som bilinnehavet. Varje personbil körs alltså i genomsnitt kortare sträcka år 1994 jämfört med 1978. En omfördelning av bilresorna har dock skett så att en högre andel av resorna sker som förare medan andelen av resorna som sker som bilpassagerare minskat. - Bilanvändningen visar samma mönster som bilinnehavet, de yngsta reser mindre med bil medan de äldres bilanvändning ökat. För majoriteten av befolkningen sker små förändringar. Den ekonomiska utvecklingen är central för framtida förändringar av bilinnehav och bilanvändning i Sverige. Den utplaning av tillväxten som skett hittills under 1990-talet kan snabbt ändras till en betydligt högre tillväxt om den privata konsumtionen åter ökar. Många som hittills under 1990-talet skjutit upp att ta körkort eller köpa bil skulle säkerligen göra det om de fick råd till det. En fortsatt svag ekonomisk utvecklingen leder dock på sikt till att nya grupper av körkorts- och billösa kommer att uppstå i Sverige.
description: Proceedings from the Annual Transport Conference at Aalborg University, Vol. 3 No. 1 (1996): Proceedings from the Annual Transport Conference at Aalborg University
NESTED SUBSETS, SCALE, AND THE DISTRIBUTION OF ABUNDANCE: A MACROECOLOGICAL APPROACH
Creators: name: Baldridge, Elita
Publisher: name: figshare
Year: 2014
Subjects: subject: Ecology
subject: FOS: Biological sciences

description: I have decided not to submit the contents of this thesis to a journal, because I did not follow best practices extracting the data for the thesis, and did not know better at the time (did not check to be certain that the site had a good run for the year, made all matrices by hand). However, I do not think that the mistakes affected the conclusions in my thesis. If anything, the errors would have made it less likely to observe the pattern.
Simulering af miljøkonsekvenser ved togkørsel
Creators: name: Winge, Ulrik
Publisher: name: Proceedings from the Annual Transport Conference at Aalborg University
Year: 2020

description: Miljøspørgsmålet får større og større vægt i den trafikpolitiske debat, og jernbanen spiller en fremtrædende rolle når det gælder miljømæssigt mere bæredygtig transport. Men også togenes energiforbrug og luftforurening pr transporteret enhed kan nedbringes. Det kan ske bl.a. ved at påvirke den måde togene kører på. Imidlertid har der hidtil ikke eksisteret generelle værktøjer, der har kunnet belyse konsekvenserne af ændrede køremønstre, infrastruktur og materielanvendelse. Derfor har Banestyrelsen og DSB udviklet et simuleringsværktøj, der gør det muligt at beregne energiforbrug og luftforurening fra tog i forskellige driftssituationer. Værktøjet er udviklet som støtteværktøj for ingeniører og øvrige relevante fagfolk. Første udgave af værktøjet er nu stort set færdigudviklet, dog mangler endnu en afsluttende verifikation af beregningernes rigtighed. Med værktøjet er det muligt at simulere miljøeffekten ved ændringer af materielanvendelse, infrastruktur, køreplaner, standsemønstre og kørestrategier. Værktøjet planlægges efterfølgende udbygget med relevante litra (togtyper) der anvendes ved togtransport i Danmark, og vil blive anvendt i forbindelse med større projekter, til undervisningsformål samt til at beregne generelle sammenhænge mellem energiforbrug/emissioner og togstørrelse, trækkraft, køreplan mv.
description: Proceedings from the Annual Transport Conference at Aalborg University, Vol. 4 No. 1 (1997): Proceedings from the Annual Transport Conference at Aalborg University
Synthesis of New 5?-Norcarbocyclic Aza/Deaza Purine Fleximers - Noncompetitive Inhibitors of E.coli Purine Nucleoside Phosphorylase
Creators: name: Khandazhinskaya, Anastasia
name: Fateev, Ilja
name: Konstantinova, Irina
name: Esipov, Roman
name: Polyakov, Konstantin
name: Seley-Radtke, Katherine
name: Kochetkov, Sergey
name: Matyugina, Elena
Publisher: name: Frontiers
Year: 2022

description: A new series of flexible 5?-norcarbocyclic aza/deaza-purine nucleoside analogs were synthesized from 6-oxybicyclo[3.1.0.]hex-2-ene and pyrazole-containing fleximer analogs of heterocyclic bases using the Trost procedure. The compounds were evaluated as potential inhibitors of E. coli purine nucleoside phosphorylase. Analog 1-3 were found to be noncompetitive inhibitors with inhibition constants of 14–24 mM. From the data obtained, it can be assumed that the new 5?-norcarbocyclic nucleoside analogs interact with the active site of the PNP like natural heterocyclic bases. But at the same time the presence of a cyclopentyl moiety with 2? and 3? hydroxyls is necessary for the inhibitory properties, since compounds 8–10, without those groups did not exhibit an inhibitory effect under the experimental conditions.
Cosmos Fluid theory
Creators: name: Kitchen, Kris
Publisher: name: figshare
Year: 2014
Subjects: subject: Interstellar and Intergalactic Matter
subject: Instrumentation, Techniques, and Astronomical Observations
subject: 20104 Galactic Astronomy
subject: FOS: Physical sciences
subject: Cosmology
subject: Planetary Geology
subject: Stellar Astronomy
subject: Stars, Variable Stars
subject: Astrophysics
subject: Planetary Science
subject: Solar System, Solar Physics, Planets and Exoplanets
subject: Extragalactic Astronomy
subject: Space Science

description: Cosmos Fluid theory
The Influence of Open Access on Monograph Sales : The experience at Amsterdam University Press
Creators: name: Snijder, Ronald
Publisher: name: figshare
Year: 2014
Subjects: subject: Library and Information Studies
subject: FOS: Media and communications

description: The hybrid model of Amsterdam University
Press (AUP) combines monograph
sales with Open Access publishing. This
paper investigates the effects of Open
Access publishing on the sales of monographs,
taking into account the influence
of: commercial potential; frontlist and
backlist; and language. The data set contains
sales figures of 513 books, spread
over 36 months: 2010 to 2012. Over 70 per
cent of those books are published on Open
Access and are distributed through the
OAPEN Library. Each influence is relevant,
which makes it harder to single out the
effects of Open Access. The large difference
between frontlist sales figures and
those of the backlist leads to a separate
analysis. The frontlist sales are affected
by a combination of commercial potential
and language; Open Access publishing has
no effect in this situation. For the backlist,
Open Access publishing is a significant influence
on sales only in the subset of books
whose print run is between one and 2000.
No significant effect on books with a print
run of zero, or on books with a print run between
2001 and 3000, could be measured.
The hybrid model does not lead to more
sales of monographs available on Open
Access, and the loss of sales is negligible.
The data suggest that a hybrid model is not
an option to improve the sustainability of
monograph publishing.
title: Design and Synthesis of Lamivudine and Telbivudine Analogues
title: The Design And Synthesis Of Fleximer Analogues Of Telbivudine And Lamivudine
titleType: AlternativeTitle
Creators: name: Sparr, Owen
name: Pipher, Grayson
name: Seley-Radtke, Katherine
Publisher: name: Maryland Shared Open Access Repository
Year: 2022
Subjects: Fleximers
Reverse Fleximers
Synthesis of Flex-3TC
Synthesis of Flex-LdT Analogs

description: UMBC 26th Undergraduate Research and Creative Achievement Day (URCAD), April 18-24, 2022
Riflessioni e riflessi sulla comunicazione politica: la formazione del Governo dopo le elezioni politiche del 2018
Creators: name: Felaco, Cristiano
name: Marrazzo, Francesco
name: Mazza, Rocco
name: Punziano, Gabriella
name: Saracino, Barbara
Publisher: name: SocietàMutamentoPolitica
Year: 2020

description: The first Conte Government took office on the 1st of June, 2018, almost ninety days after the national elections held on the 4th of March: it is the longest period in the history of the Italian Republic between the date of the elections and the birth of a government. What happened to the political communication during this long period? By using content and statistical text analysis, three channels of political communication were analysed: online newspapers, social media and official speeches at the end of the consultations. The intent of the article is to examine some typical dynamics of the new ecosystem of political communication that are strictly linked, in other words: the increasing of disintermediation; the hybridization of institutional, media and social communication spheres; the supposed end of the mediation role of information professionals. This aim was raised also by placing a specific focus on the differences in communication style of the main leaders involved in the formation of the first Conte Government: Luigi Di Maio for the Five Stars Movement and Matteo Salvini for the Lega. By focusing on the entire period of the consultations and on the analysis of the contents conveyed through the various channels, it was possible to understand in depth the peculiarities of the communication strategies adopted, how they relate to each other and to targets and events. The analysis of the data highlighted the low heterogeneity of the peculiar lexicons of the various communication channels and the link between the differences in communication styles, as well as the means by which information and the temporal dimension are conveyed; in addition, it fosters to identify the differences between the main leaders Di Maio and Salvini on these aspects.
description: SocietàMutamentoPolitica, Vol 10, N° 20 (2019): Sociological Beruf : A Ten-Year Interpretive Journey
Design and Synthesis of Flex AT-527 as a Potential Antiviral Therapeutic
Creators: name: Carlyle, Evan
name: Waters, Charlie
name: Seley-Radtke, Katherine
Publisher: name: Maryland Shared Open Access Repository
Year: 2022
Subjects: AT-527
Flex-AT-527

description: UMBC 26th Undergraduate Research and Creative Achievement Day (URCAD), April 18-24, 2022
description: One outcome from the COVID-19 pandemic was the realization that we desperately need access to broad-spectrum antivirals that can be readily stockpiled and distributed to quickly treat infected individuals. Nucleoside analogues are sought after for this reason, as they have shown significant activity as a class of antivirals for decades. The Seley-Radtke group has focused on modifying the bicyclic purine base moiety of nucleoside analogues by incorporating a carbon-carbon single bond between the two heterocyclic components, endowing the nucleobase with flexibility. As a result, the fleximers can adopt a variety of favorable conformations thereby allowing the compound to exhibit potent antiviral activity not seen in the rigid-parent nucleoside. In addition, this allows for the ability to overcome antiviral resistance, as well as to be recognized by various viral enzymes, resulting in significant activity against a wide variety of viruses. AT-527 is a nucleoside analogue originally designed to treat Hepatitis C Virus (HCV), that has shown activity against SARS-CoV-2 and other viruses. The aim of this project is to incorporate the fleximer technology into the AT-527 scaffold, thereby potentially expanding its biological scope. Computational docking studies were carried out to explore the binding potential for a series of AT analogues, as well as to guide future SAR studies. The synthesis of the parent Flex-AT-527 was completed in ten steps, with each step having fair to good yields. The results of this project are reported herein.