title: Ð¢ÐµÑ Ð½Ð¾Ð»Ð¾Ð³Ð¸Ñ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ Ð¼ÑÐ»ÑÑÐ¸ÑÐ»Ð¾Ð¹Ð½ÑÑ Ð¿Ð¾ÐºÑÑÑÐ¸Ð¹ Ð½Ð° Ð¿Ð¾Ð²ÐµÑÑ Ð½Ð¾ÑÑÐ¸ Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸Ñ Ð´Ð»Ñ ÑÐ»ÑÑÑÐµÐ½Ð¸Ñ Ð¸Ñ Ð±Ð¸Ð¾ÑÐ¾Ð²Ð¼ÐµÑÑÐ¸Ð¼Ð¾ÑÑÐ¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Technology of obtaining multilayer coatings on the surface of nanoparticles for improvement of biocompatibility
Creators: name: Ð¥ÑÑÐ¾ÑÑÐ½, ÐÐ»ÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2021
Subjects: Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸ÑÑ Ð½Ð° Ð¾ÑÐ½Ð¾Ð²Ðµ Ð´Ð¸Ð¾ÐºÑÐ¸Ð´Ð° ÐºÑÐµÐ¼Ð½Ð¸Ñ
Ð¼ÑÐ»ÑÑÐ¸ÑÐ»Ð¾Ð¹Ð½ÑÐµ Ð¿Ð¾ÐºÑÑÑÐ¸Ñ
Ñ Ð°ÑÐ°ÐºÑÐµÑÐ¸Ð·Ð°ÑÐ¸Ñ
Ð³ÐµÐ¼Ð¾Ð»Ð¸ÑÐ¸ÑÐµÑÐºÐ°Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ
Ð±Ð¸Ð¾ÑÐ¾Ð²Ð¼ÐµÑÑÐ¸Ð¼Ð¾ÑÑÑ
Ð¶Ð¸Ð·Ð½ÐµÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ ÐºÑÐ»ÑÑÑÑ
silicon dioxide-based nanoparticles
multilayer coatings
characterization
hemolytic activity
biocompatibility
cell culture viability

description: Ð Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÐµ Ð±ÑÐ»Ð¸ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ñ Ð¿ÑÐ¾ÑÐ¾ÐºÐ¾Ð»Ñ Ð½Ð°Ð½ÐµÑÐµÐ½Ð¸Ñ Ð¼ÑÐ»ÑÑÐ¸ÑÐ»Ð¾Ð¹Ð½ÑÑ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ½ÑÑÐ¿Ð¾Ð»Ð¸-L-Ð°ÑÐ³Ð¸Ð½Ð¸Ð½Ð° Ð¸ Ð´ÐµÐºÑÑÑÐ°Ð½Ð° (SiTi@PARG@DEX)Ð¿Ð¾ÐºÑÑÑÐ¸Ð¹ Ð½Ð° Ð¿Ð¾Ð²ÐµÑÑÐ½Ð¾ÑÑÑ Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸Ñ(ÐÐ§)Ð´Ð¸Ð¾ÐºÑÐ¸Ð´Ð° ÐºÑÐµÐ¼Ð½Ð¸Ñ Ñ Ð¼ÐµÑÐ°Ð»Ð»Ð¸ÑÐµÑÐºÐ¸Ð¼ ÑÐ»Ð¾ÐµÐ¼ Ð´Ð¸Ð¾ÐºÑÐ¸Ð´Ð° ÑÐ¸ÑÐ°Ð½Ð° Ð½Ð° Ð¿Ð¾Ð²ÐµÑÑÐ½Ð¾ÑÑÐ¸. ÐÐ»Ñ Ð´Ð¾ÑÑÐ¸Ð¶ÐµÐ½Ð¸Ñ Ð¿Ð¾ÑÑÐ°Ð²Ð»ÐµÐ½Ð½ÑÑ Ð·Ð°Ð´Ð°Ñ, Ð±ÑÐ»Ð¸ Ð¸Ð·ÑÑÐµÐ½Ñ ÑÐ¶Ðµ Ð¸Ð¼ÐµÑÑÐ¸ÐµÑÑ Ð¿ÑÐ¾ÑÐ¾ÐºÐ¾Ð»Ñ, ÐºÐ¾ÑÐ¾ÑÑÐµ Ð±ÑÐ»Ð¸ Ð¾Ð¿ÑÐ¸Ð¼Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°Ð½Ñ Ð¿Ð¾Ð´ ÐºÐ¾Ð½ÐºÑÐµÑÐ½ÑÑ Ð·Ð°Ð´Ð°ÑÑ. Ð¢Ð°ÐºÐ¶Ðµ Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»Ð°ÑÑ Ð¾ÑÐµÐ½ÐºÐ° Ð±Ð¸Ð¾ÑÐ¾Ð²Ð¼ÐµÑÑÐ¸Ð¼Ð¾ÑÑÐ¸ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ ÐÐ§Ð¿ÑÐ¸Ð¿Ð¾Ð¼Ð¾ÑÐ¸ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ¾Ð² Ð½Ð° Ð³ÐµÐ¼Ð¾Ð»Ð¸ÑÐ¸ÑÐµÑÐºÑÑ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÐÐ§Ð¸ Ð¶Ð¸Ð·Ð½ÐµÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ð¿ÑÐ¸ Ð´Ð¾Ð±Ð°Ð²Ð»ÐµÐ½Ð¸Ð¸ ÐÐ§.ÐÑÐ»Ð¾ ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¾, ÑÑÐ¾ Î¶-Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»Ñ Ð² Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼Ð¾ÑÑÐ¸ Ð¾Ñ ÑÑÐ°Ð¿Ð° ÑÐ¸Ð½ÑÐµÐ·Ð° Ð·Ð½Ð°ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾ Ð¾ÑÐ»Ð¸ÑÐ°Ð»Ð¸ÑÑ, ÑÑÐ¾ ÐºÐ¾ÑÐ²ÐµÐ½Ð½Ð¾ Ð¿Ð¾Ð´ÑÐ²ÐµÑÐ¶Ð´Ð°Ð»Ð¾ Ð¾ÑÐ°Ð¶Ð´ÐµÐ½Ð¸Ðµ Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ½ÑÑ Ð¿Ð¾ÐºÑÑÑÐ¸Ð¹, Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ ÑÐµÑÐ¼Ð¾Ð³ÑÐ°Ð²Ð¸Ð¼ÐµÑÑÐ¸Ð¸ Ð½Ð°Ð½ÐµÑÐµÐ½Ð¸Ðµ Ð±ÑÐ»Ð¾ Ð´Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾ Ð¸ Ð¾ÐºÐ°Ð·Ð°Ð»Ð¾ÑÑ, ÑÑÐ¾ Ð¼Ð°ÑÑÐ° Ð½Ð°Ð½Ð¾ÑÐ¸Ð¼ÑÑ ÑÐ»Ð¾ÐµÐ² ÑÐ¾ÑÑÐ°Ð²Ð¸Ð»Ð° Ð¾ÐºÐ¾Ð»Ð¾ 10% Ð¾Ñ Ð¼Ð°ÑÑÑ ÐÐ§.Ð ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±Ð¸Ð¾ÑÐ¾Ð²Ð¼ÐµÑÑÐ¸Ð¼Ð¾ÑÑÐ¸, Ð±ÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ ÐÐ§ SiTi@PARG@DEXÐ½Ðµ Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÑÑ Ð·Ð½Ð°ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ð¹ Ð³ÐµÐ¼Ð¾Ð»Ð¸ÑÐ¸ÑÐµÑÐºÐ¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ (Ð³ÐµÐ¼Ð¾Ð»Ð¸Ð· Ð² Ð¿ÑÐµÐ´ÐµÐ»Ð°Ñ 10%) Ð² ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸ÑÑ Ð´Ð¾ 3Ð¼Ð³/Ð¼Ð» Ð¿Ð¾ÑÐ»Ðµ Ð¸Ð½ÐºÑÐ±Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð½Ð° Ð¿ÑÐ¾ÑÑÐ¶ÐµÐ½Ð¸Ð¸ 2 ÑÐ°ÑÐ¾Ð².SiTi@PARG@DEXÐÐ§ Ð¿Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÑÑ Ð»ÑÑÑÐ¸Ðµ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð¿Ð¾ ÑÑÐ°Ð²Ð½ÐµÐ½Ð¸Ñ Ñ ÐÐ§ SiO2@TiO2Ð±ÐµÐ· Ð¿Ð¾Ð»Ð¸Ð¼ÐµÑÐ½ÑÑ Ð¿Ð¾ÐºÑÑÑÐ¸Ð¹ ÐºÐ°ÑÐ°ÑÐµÐ»ÑÐ½Ð¾ Ð¶Ð¸Ð·Ð½ÐµÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÐ¸ ÐºÐ»ÐµÑÐ¾Ðº Ð¿ÑÐ¸ Ð¸Ð½ÐºÑÐ±Ð°ÑÐ¸Ð¸ Ð½Ð° Ð¿ÑÐ¾ÑÑÐ¶ÐµÐ½Ð¸Ð¸ ÑÑÑÐ¾Ðº. ÐÑÐ¸ ÑÑÐ¾Ð¼ ÑÑÐ¾Ð²ÐµÐ½Ñ Ð¶Ð¸Ð·Ð½ÐµÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÐ¸ Ð¾ÐºÐ°Ð·Ð°Ð»ÑÑ Ð²ÑÑÐµ Ð½Ðµ ÑÐ¾Ð»ÑÐºÐ¾, ÑÐµÐ¼ Ñ SiO2@TiO2ÐÐ§, Ð½Ð¾ Ð¸ ÑÐµÐ¼ Ñ ÑÑÐ°Ð½Ð´Ð°ÑÑÐ½ÑÑ ÐÐ.
description: In this work, we applied protocols for multilayer polymer poly-L-arginine and dextran (SiTi@PARG@DEX) coating onthe surface of silicon dioxide nanoparticles with a metallic layer of titanium dioxide on the surface. To achieve these goals, we studied the existing protocols, which were optimized for a specific task.The biocompatibility of the obtained nanoparticles was also evaluated by experiments on the hemolytic activity of NP( nanoparticles)and cell viability when NP were added.It was found that the zeta potentials were significantly different depending on the synthesis stage, which indirectly confirmed the deposition of polymer coatings, the application was proved by thermogravimetry and it turned out that the mass of the applied layers was about 10% of the mass of the nanoparticle.During the biocompatibility study, it was shown that theNPSiTi@PARG@DEX do not have significant hemolytic activity (hemolysis within 10%) in concentrations up to 3 mg/ml after incubation for 2 hours.SiTi@PARG@DEX NPshow better results compared to SiO2@TiO2NPswithout polymer coatings in terms of cell viability during incubation during the day. At the same time, the level of viability was higher not only than that of SiO2@TiO2NP, but also than that of standard SiNP.