title: ÐÐµÑÐ¾Ð´ Ð¾Ð±ÑÐ°ÑÐ¸Ð¼Ð¾Ð¹ ÑÐ¾ÑÐ±ÑÐ¸Ð¸ Ð²Ð½ÐµÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð½Ð°Ð½Ð¾Ð²ÐµÐ·Ð¸ÐºÑÐ» Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð°Ð¿ÑÐ°Ð¼ÐµÑÐ¾Ð²
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: The method of reversible sorption of extracellular nanovesicles using aptamers
Creators: name: Ð ÑÐ¼Ð¾Ð²Ð°, ÐÐ½Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð²Ð½ÐµÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐµ Ð½Ð°Ð½Ð¾Ð²ÐµÐ·Ð¸ÐºÑÐ»Ñ
ÐÐÐ-Ð°Ð¿ÑÐ°Ð¼ÐµÑ
Ð¾Ð±ÑÐ°ÑÐ¸Ð¼Ð°Ñ ÑÐ¾ÑÐ±ÑÐ¸Ñ
extracellular nanovesicles
DNA aptamer
reversible sorption

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¾Ð¿ÑÐ¸Ð¼Ð¸Ð·Ð°ÑÐ¸Ð¸ ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¾Ð±ÑÐ°ÑÐ¸Ð¼Ð¾Ð¹ ÑÐ¾ÑÐ±ÑÐ¸Ð¸ Ð²Ð½ÐµÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð½Ð°Ð½Ð¾Ð²ÐµÐ·Ð¸ÐºÑÐ» Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ Ð°Ð¿ÑÐ°Ð¼ÐµÑÐ¾Ð². ÐÐ°Ð´Ð°ÑÐ¸, ÐºÐ¾ÑÐ¾ÑÑÐµ ÑÐµÑÐ°Ð»Ð¸ÑÑ Ð² ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ: - Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð¸Ðµ ÑÐ¾ÑÐ°Ð»ÑÐ½Ð¾Ð¹ Ð¿Ð¾Ð¿ÑÐ»ÑÑÐ¸Ð¸ Ð²Ð½ÐµÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð½Ð°Ð½Ð¾Ð²ÐµÐ·Ð¸ÐºÑÐ» Ð¿Ð»Ð°Ð·Ð¼Ñ ÐºÑÐ¾-Ð²Ð¸. ÐÐ½Ð°Ð»Ð¸Ð· ÐºÐ°ÑÐµÑÑÐ²ÐµÐ½Ð½ÑÑ Ð¸ ÐºÐ¾Ð»Ð¸ÑÐµÑÑÐ²ÐµÐ½Ð½ÑÑ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸Ðº Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÑ Ð½Ð°Ð½Ð¾Ð²Ðµ-Ð·Ð¸ÐºÑÐ»; - ÑÐµÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ð¼Ð°Ð³Ð½Ð¸ÑÐ½ÑÑ ÑÐ¾ÑÐ±ÐµÐ½ÑÐ¾Ð²; - Ð¾Ð¿ÑÐ¸Ð¼Ð¸Ð·Ð°ÑÐ¸Ñ ÑÐ¾ÑÐ±ÑÐ¸Ð¸ Ð¸ Ð´ÐµÑÐ¾ÑÐ±ÑÐ¸Ð¸ Ð²Ð½ÐµÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ Ð½Ð°Ð½Ð¾Ð²ÐµÐ·Ð¸ÐºÑÐ» Ð½Ð° ÐÐÐ-Ð°Ð¿ÑÐ°Ð¼ÐµÑ. ÐÐ±ÑÐµÐºÑ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ â Ð²Ð½ÐµÐºÐ»ÐµÑÐ¾ÑÐ½ÑÐµ Ð½Ð°Ð½Ð¾Ð²ÐµÐ·Ð¸ÐºÑÐ»Ñ Ð¿Ð»Ð°Ð·Ð¼Ñ ÐºÑÐ¾Ð²Ð¸. Ð Ð°Ð±Ð¾ÑÐ° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ Ð½Ð°ÑÑÐ½Ð¾Ð¹ Ð»Ð°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¸ ÑÑÐ±ÐºÐ»ÐµÑÐ¾ÑÐ½ÑÑ ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¹ Ñ Ð³ÑÑÐ¿Ð¿Ð¾Ð¹ Ð¾Ð½ÐºÐ¾-ÑÐ½Ð´Ð¾ÐºÑÐ¸Ð½Ð¾Ð»Ð¾Ð³Ð¸Ð¸ (Ð¡ÐÐ¢-ÐÐ­) ÐÐÐÐ¦ Ð¾Ð½ÐºÐ¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¸Ð¼. Ð.Ð. ÐÐµÑÑÐ¾Ð²Ð°. Ð ÑÐ¾Ð´Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¾ ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¾, ÑÑÐ¾: Ð¼ÐµÑÐ¾Ð´ ÑÐ»ÑÑÑÐ°ÑÐµÐ½ÑÑÐ¸ÑÑÐ³Ð¸ÑÐ¾Ð²Ð°-Ð½Ð¸Ñ Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÐµÑ Ð²ÑÐ´ÐµÐ»Ð¸ÑÑ ÑÐ¾ÑÐ°Ð»ÑÐ½ÑÑ Ð¿Ð¾Ð¿ÑÐ»ÑÑÐ¸Ñ ÐÐÐ ÑÐ°Ð·Ð¼ÐµÑÐ¾Ð¼ 50-150 Ð½Ð¼. ÐÑ-Ð±Ð¾Ñ Ð¼Ð°Ð³Ð½Ð¸ÑÐ½ÑÑ ÑÐ°ÑÑÐ¸Ñ Ð´Ð»Ñ ÑÐ¾ÑÐ±ÑÐ¸Ð¸ ÐÐÐ Ð½Ð° ÐºÐ¾Ð¼Ð¿Ð»ÐµÐºÑ Â«Ð¼Ð°Ð³Ð½Ð¸ÑÐ½ÑÐµ ÑÐ°ÑÑÐ¸ÑÑ-ÐÐÐ-Ð°Ð¿ÑÐ°Ð¼ÐµÑÂ» Ð¾Ð±ÑÑÐ»Ð¾Ð²Ð»ÐµÐ½ Ð¸Ñ ÑÐ¾ÑÑÐ°Ð²Ð¾Ð¼ Ð¸ ÑÐ¾ÑÐ±Ð¸ÑÑÑÑÐµÐ¹ ÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÑÑ. ÐÐ°Ð¸Ð»ÑÑÑÐ¸Ð¼ Ð¼Ð°Ð³Ð½Ð¸ÑÐ½ÑÐ¼ ÑÐ¾ÑÐ±ÐµÐ½ÑÐ¾Ð¼ Ð´Ð»Ñ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ Ð¾Ð±ÑÐ°ÑÐ¸Ð¼Ð¾Ð¹ ÑÐ¾ÑÐ±ÑÐ¸Ð¸ Ð²Ð½ÐµÐºÐ»Ðµ-ÑÐ¾ÑÐ½ÑÑ Ð½Ð°Ð½Ð¾Ð²ÐµÐ·Ð¸ÐºÑÐ» ÑÐ²Ð»ÑÑÑÑÑ ÑÐ°ÑÑÐ¸ÑÑ SILEKS. ÐÐ¿ÑÐ¸Ð¼Ð°Ð»ÑÐ½ÑÐ¼Ð¸ ÑÑÐ»Ð¾Ð²Ð¸ÑÐ¼Ð¸ Ð´Ð»Ñ Ð´ÐµÑÐ¾ÑÐ±ÑÐ¸Ð¸ Ð²ÐµÐ·Ð¸ÐºÑÐ» ÑÐ²Ð»ÑÑÑÑÑ: ÑÐµÐ¼Ð¿ÐµÑÐ°ÑÑÑÐ° 25 Â°Ð¡ (ÐºÐ¾Ð¼Ð½Ð°ÑÐ½Ð°Ñ), ÐºÐ¾Ð½ÑÐµÐ½ÑÑÐ°ÑÐ¸ÐµÐ¹ NaCl 300 Ð¼Ð. ÐÑÐ¸ Ð½Ð°Ð¿Ð¸ÑÐ°Ð½Ð¸Ð¸ Ð»Ð¸ÑÐµÑÐ°ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¾Ð±Ð·Ð¾ÑÐ° Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ ÑÐ»ÐµÐ´ÑÑÑÐ¸Ðµ Ð±Ð°Ð·Ñ Ð´Ð°Ð½Ð½ÑÑ: PubMed. Ð¡ÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÑÑ Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ°Ð»ÑÐ½ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¾ÑÑÑÐµÑÑÐ²Ð»ÑÐ»Ð¸ Ñ Ð¸Ñ-Ð¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¿Ð°ÐºÐµÑÐ° Ð¿ÑÐ¾Ð³ÑÐ°Ð¼Ð¼: Microsoft Excel, Microsoft Word.
description: This work is devoted to optimizing the technology of reversible sorption of ex-tracellular nanovesicles using aptamers. The tasks that were solved during the study: - isolation of a total population of extracellular plasma nanovesicles. Analysis of the qualitative and quantitative characteristics of the obtained nanovesicles; - testing of magnetic sorbents; - optimization of sorption and desorption of extracellular nanovesicles on DNA aptamer. The object of the study is extracellular nanovesicles of blood plasma. The work was performed on the basis of the scientific laboratory of subcellular technologies with the Group of Onco-Endocrinology (SCT-OE) of the N.N. Petrov National Research Institute of Oncology. During the study, it was found that: the ultracentrifugation method makes it pos-sible to isolate a total population of VNV with a size of 50-150 nm. The selection of magnetic particles for the sorption of VNV on the complex "magnetic particles-DNA-aptamer" is due to their composition and sorption ability. SILEKS particles are the best magnetic sorbent for conducting reversible sorption of extracellular nanovesicles. The optimal conditions for desorption of vesicles are: temperature 25 Â°C (room), NaCl concentration 300 mm. When writing the literary review, the following databases were used: PubMed. Statistical processing of experimental data was carried out using a software package: Microsoft Word.