Genetic tool development in marine protists: emerging model organisms for experimental cell biology
Creators: name: Faktorová, Drahomíra
name: Nisbet, R. Ellen R.
name: Fernández Robledo, José A.
name: Casacuberta, Elena
name: Sudek, Lisa
name: Allen, Andrew E.
name: Ares, Manuel
name: Aresté, Cristina
name: Balestreri, Cecilia
name: Barbrook, Adrian C.
name: Beardslee, Patrick
name: Bender, Sara
name: Booth, David S.
name: Bouget, François-Yves
name: Bowler, Chris
name: Breglia, Susana A.
name: Brownlee, Colin
name: Burger, Gertraud
name: Cerutti, Heriberto
name: Cesaroni, Rachele
name: Chiurillo, Miguel A.
name: Clemente, Thomas
name: Coles, Duncan B.
name: Collier, Jackie L.
name: Cooney, Elizabeth C.
name: Coyne, Kathryn
name: Docampo, Roberto
name: Dupont, Christopher L.
name: Edgcomb, Virginia
name: Einarsson, Elin
name: Elustondo, Pía A.
name: Federici, Fernan
name: Freire-Beneitez, Veronica
name: Freyria, Nastasia J.
name: Fukuda, Kodai
name: García, Paulo A.
name: Girguis, Peter R.
name: Gomaa, Fatma
name: Gornik, Sebastian G.
name: Guo, Jian
name: Hampl, Vladimír
name: Hanawa, Yutaka
name: Haro-Contreras, Esteban R.
name: Hehenberger, Elisabeth
name: Highfield, Andrea
name: Hirakawa, Yoshihisa
name: Hopes, Amanda
name: Howe, Christopher J.
name: Hu, Ian
name: Ibañez, Jorge
name: Irwin, Nicholas A. T.
name: Ishii, Yuu
name: Janowicz, Natalia Ewa
name: Jones, Adam C.
name: Kachale, Ambar
name: Fujimura-Kamada, Konomi
name: Kaur, Binnypreet
name: Kaye, Jonathan Z.
name: Kazana, Eleanna
name: Keeling, Patrick J.
name: King, Nicole
name: Klobutcher, Lawrence A.
name: Lander, Noelia
name: Lassadi, Imen
name: Li, Zhuhong
name: Lin, Senjie
name: Lozano, Jean-Claude
name: Luan, Fulei
name: Maruyama, Shinichiro
name: Matute, Tamara
name: Miceli, Cristina
name: Minagawa, Jun
name: Moosburner, Mark
name: Najle, Sebastián R.
name: Nanjappa, Deepak
name: Nimmo, Isabel C.
name: Noble, Luke
name: Novák Vanclová, Anna M. G.
name: Nowacki, Mariusz
name: Nuñez, Isaac
name: Pain, Arnab
name: Piersanti, Angela
name: Pucciarelli, Sandra
name: Pyrih, Jan
name: Rest, Joshua S.
name: Rius, Mariana
name: Robertson, Deborah
name: Ruaud, Albane
name: Ruiz-Trillo, Iñaki
name: Sigg, Monika A.
name: Silver, Pamela A.
name: Slamovits, Claudio H.
name: Jason Smith, G.
name: Sprecher, Brittany N.
name: Stern, Rowena
name: Swart, Estienne C.
name: Tsaousis, Anastasios D.
name: Tsypin, Lev
name: Turkewitz, Aaron
name: Turnšek, Jernej
name: Valach, Matus
name: Vergé, Valérie
name: Von Dassow, Peter
name: Von Der Haar, Tobias
name: Waller, Ross F.
name: Wang, Lu
name: Wen, Xiaoxue
name: Wheeler, Glen
name: Woods, April
name: Zhang, Huan
name: Mock, Thomas
name: Worden, Alexandra Z.
name: Lukeš, Julius
Publisher: name: Apollo - University of Cambridge Repository
Year: 2020
Subjects: Resource
/631/1647/767/722
/631/337
resource

description: Abstract: Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways.