Additional file 1 of Targeted inhibition of the PI3K/AKT/mTOR pathway by (+)-anthrabenzoxocinone induces cell cycle arrest, apoptosis, and autophagy in non-small cell lung cancer
Creators: name: Li, Xiao-Qian
name: Cheng, Xiao-Ju
name: Wu, Jie
name: Wu, Kai-Feng
name: Liu, Tie
Publisher: name: figshare
Year: 2024
Subjects: subject: Biochemistry
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: Cell Biology
subject: Molecular Biology
subject: Pharmacology
subject: Chemical Sciences not elsewhere classified
subject: Cancer
subject: Infectious Diseases
subject: FOS: Health sciences

description: Additional file 1. Includes the primer sequences in qRT-PCR assay; effects of different concentrations of (+)-ABX on the colony formation of three NSCLC cell lines; effects of different concentrations of (+)-ABX on migration of three NSCLC cell lines; effects of different concentrations of (+)-ABX on invasion of three NSCLC cell lines; effects of different concentrations of (+)-ABX on the cell cycle distribution of three NSCLC cell lines; flow cytometry analysis of apoptosis rates in three NSCLC cell lines treated with different concentrations of (+)-ABX; transcriptomic analysis; the images of immunohistochemistry staining in three repetitions; and the images of the original western blots in three repetitions. Fig. S1. Antitumor activity screening results of (+)-ABX. The screening range includes nasopharyngeal carcinoma cells (CNE1), colorectal cancer cells (LOVO), thyroid cancer cells (TPC-1), melanoma cells (B16), liver cancer cells (MHCC97H, Hep1), and lung cancer cells (A549, H1299, and H226). The IC50 values were determined using the CCK-8 method. Table S1. The primer sequences in qRT-PCR assay. Fig. S2. Effects of different concentrations of (+)-ABX on the colony formation of three NSCLC cell lines. Fig. S3. Effects of different concentrations of (+)-ABX on migration of three NSCLC cell lines (magnification ×200; scale bar, 50 μm). Fig. S4. Effects of different concentrations of (+)-ABX on invasion of three NSCLC cell lines (magnification × 200; scale bar, 50 μm). Fig. S5. Effects of different concentrations of (+)-ABX on the cell cycle distribution of three NSCLC cell lines. Fig. S6. Flow cytometry analysis of apoptosis rates in three NSCLC cell lines treated with different concentrations of (+)-ABX. Fig. S7. Transcriptomic analysis. Group A: untreated group; group B: 20 μM (+)-ABX treated group; group C: 40 μM (+)-ABX treated group. A Principal component analysis (PCA). B Volcano plot of differentially expressed genes (A versus B). C Volcano plot of differentially expressed genes (B vs C). D Venn diagram of differentially expressed genes. E Hierarchical clustering heatmap of differentially expressed genes in three groups. F Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis (A vs B). G Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis (B versus C). Fig. S8. Differences in the binding sites between (+)-ABX and LY294002 with the PI3K protein. (A) Docking results of (+)-ABX with PI3K. (B) Docking results of LY294002 with PI3K. Table S2. The images of immunohistochemistry staining in three repetitions. Table S3. The images of the original western blots in three repetitions.