MOESM1 of The peptide transporter 1a of the zebrafish Danio rerio, an emerging model in nutrigenomics and nutrition research: molecular characterization, functional properties, and expression analysis
Creators: name: Vacca, Francesca
name: Barca, Amilcare
name: Gomes, Ana
name: Mazzei, Aurora
name: Piccinni, Barbara
name: Cinquetti, Raffaella
name: Vecchio, Gianmarco
name: Romano, Alessandro
name: Rønnestad, Ivar
name: Bossi, Elena
name: Verri, Tiziano
Publisher: name: figshare
Year: 2019
Subjects: subject: Biochemistry
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Physiology
subject: Evolutionary Biology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: FOS: Chemical sciences
subject: Ecology
subject: Immunology
subject: FOS: Clinical medicine
subject: FOS: Clinical medicine
subject: 69999 Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Inorganic Chemistry
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: FOS: Health sciences

description: Additional file 1: Table S1. List of the specific primers used for cloning and qPCR analysis. Sequence accession numbers, primer sequences and amplicon sizes are shown. Figure S1. Nucleotide and predicted amino acid sequence of zebrafish pept1a (slc15a1a) obtained using ORFfinder (https://www.ncbi.nlm.nih.gov/orffinder/). Numbers on the left refer to the nucleotide (upper row) and amino acid (lower row) positions. Nucleotides are numbered, starting from the first ATG initiation codon. * indicates the stop codon. The specific primers used for cloning and PCR analyses (Additional file 1: Table S1) are indicated in red and green, respectively. In the amino acid sequence, putative transmembrane domains, obtained using the TMHMM v. 2.0 program as implemented in SMART, are indicated and named I to XII. Potential extracellular N-glycosylation sites (white boxes), potential cAMP/cGMP-dependent protein kinase phosphorylation sites at the cytoplasmic surface (light gray boxes) and potential protein kinase C phosphorylation sites at the cytoplasmic surface (dark gray boxes) were obtained using the ScanProsite tool. Figure S2. Current-voltage relationships of transport-associated currents in zebrafish PepT1a, in the presence of 3 mmol/L Gly-Gln in sodium (NaCl) saline buffer (black square) and tetramethylammonium (TMACl) saline buffer (empty circle) at pH 7.6 (see Methods for details). Values are means ± SEM from 5 oocytes from one batch each group. The transport-associated current values reported were obtained by subtracting the current recorded in the absence of the substrate to that recorded in its presence. Figure S3. Expression analysis by RT-PCR on pept1a (slc15a1a) mRNA in different sections of adult zebrafish intestine. a RT-PCR assay on cDNA templates from total RNA extracted from whole gut (gut), intestinal bulb (I. bulb), mid intestine (mid) and posterior intestine (posterior); a PCR product of ~ 350 bp related to pept1a (slc15a1a) mRNA is present in all intestinal samples; L: 1 Kb Plus DNA ladder (Thermo Fisher Scientific). b A graphic representation of the adult zebrafish gut anatomy with its major adjacent tracts.