Additional file 1 of Fecal microbiota from MRL/lpr mice exacerbates pristane-induced lupus
Creators: name: Yi, Xiaoqing
name: Huang, Cancan
name: Huang, Chuyi
name: Zhao, Ming
name: Lu, Qianjin
Publisher: name: figshare
Year: 2024
Subjects: subject: Microbiology
subject: FOS: Biological sciences
subject: Immunology
subject: FOS: Clinical medicine

description: Additional file 1: Supplementary Table 1. Primer Sequences for real-time PCR. Supplementary Table2. The alpha diversity of the FMT-Lpr, FMT-Mpj and FMT-PBS mice at different time points. Supplementary Table 3. The significantly differentiated metabolites with annotations in the KEGG databases in the pairwise comparisons among the FMT-Lpr, FMT-Mpj and FMT-PBS mice. Supplementary Figure 1. (A) Representative flow diagram of gating strategies for T and B lymphocytes in the spleen and lymph nodes. (B) Representative flow diagram of gating strategies for T and B lymphocytes in the kidney. (C) Representative flow diagram of gating strategies for T cells, B cells, innate lymphoid cells (ILCs) as well as the CD11b+ cell subsets in the large and small intestine. Supplementary Figure 2. (A) mRNA expression levels of the inflammatory cytokines in the kidney (Il6, Tnf, Il17a, Ifng). (B) Flow cytometric analysis of the CD4+, CD8+ cells gated on the CD3+ T cells, B220+ cells and plasma cells gated on the live cells in the kidney. (C) Serum anti-dsDNA IgG quantified by ELISA. (D) Flow cytometric analysis of the Th1, Th2, Th17 percentages gated on the CD4+ T cell in the spleens and lymph nodes (SP: spleen; DLN: draining lymph node; MLN: mesentery lymph nodes; PP: peyer’s patch). *p &lt; 0.05.**p&lt;0.001. FMT-Lpr, n=10~11; FMT-Mpj, n=9~10; FMT-PBS, n=6~7. Supplementary Figure 3. mRNA expression levels of IL-6, IL-12a, IL-17a in the small intestine (A) and IL-12a, IL-17a in the large intestine (B). Flow cytometric analysis of the ILC1, ILC2, ILC3 subsets percentages gated on the CD45+CD3-CD19-CD127+ cells in the small intestine (C) and the large intestine (D). Flow cytometric analysis of the CD4+ and CD8+ cell percentages gated on the CD3+ cells in the small intestine (E) and the large intestine (F). Flow cytometric analysis of the CD44+CD62L- cell percentages gated on the CD4+ T cell (G) and CD8+T cell (H). (Left: the small intestine; right: the large intestine). (I) Flow cytometric analysis of the CD11b+ cell percentages gated on the CD45+CD3-CD19- cells in the large intestine. (J) Flow cytometric analysis of the Ly6C-Ly6G+ and Ly6C+Ly6G- cell percentage gated on the CD11b+ cells in the large intestine. *p &lt; 0.05.**p&lt; 0.001. FMT-Lpr, n=8~11; FMT-Mpj, n=5~10; FMT-PBS, n=5~7. Supplementary Figure 4. (A) The Calinski-Harabasz index plot indicated two was the optimal number for clustering the enterotypes. (B) The compositions of the two enterotypes in each group (blue: enterotype1, green: enterotype2). (C) Principal coordinate analysis (PCoA) of the gut microbial community based on the metagenome data. Supplementary Figure 5. The relative abundances of Candidatus_Arthromitus, Adlercreutzia, Olsenella, Atopobium, Collinsella, Holdemania, unclassified_f__Coriobacteriaceae, Turicibacter among three groups. *p &lt; 0.05.**p&lt;0.001. FMT-Lpr, n=11; FMT-Mpj, n=10; FMT-PBS, n=7. Supplementary Figure 6. Differential bacterial taxa for each group shown by LDA score&gt; 2.0 at the species level. Supplementary Figure 7. The VIP bubble plot showed the differential metabolites ordered by the VIP scores and the corresponding expression heatmap between the FMT-Lpr and FMT-Mpj. The deeper the color is, the higher the level of the metabolite is. Supplementary Figure 8. The VIP bubble plot showed the differential metabolites ordered by the VIP scores and the corresponding expression heatmap between the FMT-Lpr and FMT-PBS. The deeper the color is, the higher the level of the metabolite is. Supplementary Figure 9. The VIP bubble plot showed the differential metabolites ordered by the VIP scores and the corresponding expression heatmap between the FMT-Mpj and FMT-PBS. The deeper the color is, the higher the level of the metabolite is. Supplementary Figure 10. The relative abundances of the ABC transporters, Protein digestion and absorption, Glycerophospholipid metabolism among the FMT-Lpr, FMT-Mpj and FMT-PBS. *p &lt; 0.05.**p&lt;0.001. FMT-Lpr, n=11; FMT-Mpj, n=10; FMT-PBS, n=7. Supplementary Figure 11. (A) The association of the abundances of the prevotella taxa with the percentage of plasma cells in the large intestine. (B)The association of the abundances of the prevotella taxa with the percentage of plasma cells in the peyer’s patches. (C)The association of the abundances of the prevotella taxa with the percentage of plasma cells in the mesentery lymph nodes. only r values&gt;0.4 and p values&lt;0.05 were shown.