title: Ð¡ÑÐ°Ð²Ð½Ð¸ÑÐµÐ»ÑÐ½ÑÐ¹ Ð°Ð½Ð°Ð»Ð¸Ð· Ð±Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð°Ð½ÑÐ¸Ð¼Ð¸ÐºÑÐ¾Ð±Ð½Ð¾Ð³Ð¾ Ð¿ÐµÐ¿ÑÐ¸Ð´Ð°, ÐµÐ³Ð¾ ÐºÐ¾Ð½ÑÑÐ³Ð°ÑÐ° Ñ Ð°Ð¼Ð¸ÐºÐ°ÑÐ¸Ð½Ð¾Ð¼ Ð¸ ÐºÐ¾Ð¼Ð±Ð¸Ð½Ð°ÑÐ¸Ð¹ Ñ Ð½Ð°Ð½Ð¾ÑÐ°ÑÑÐ¸ÑÐ°Ð¼Ð¸ ÑÐµÑÐµÐ±ÑÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: A comparative analysis of the biological activity of the antimicrobial peptide, its conjugate with amikacin and combinations with silver nanoparticles
Creators: name: ÐÐ»Ð°Ð´Ð¸Ð¼Ð¸ÑÐ¾Ð²Ð°, ÐÐ»Ð¸Ð·Ð°Ð²ÐµÑÐ°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: ÐÐµÐ¿ÑÐ¸Ð´Ñ
Ð¡ÐµÑÐµÐ±ÑÐ¾
Ð°Ð¼Ð¸ÐºÐ°ÑÐ¸Ð½
ÐºÐ¾Ð½ÑÑÐ³Ð°ÑÐ¸Ñ
ÑÐ¸Ð½ÐµÑÐ³Ð¸Ð·Ð¼
Ð°Ð½ÑÐ¸Ð¼Ð¸ÐºÑÐ¾Ð±Ð½Ð°Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ
Ð³ÐµÐ¼Ð¾Ð»Ð¸ÑÐ¸ÑÐµÑÐºÐ°Ñ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ
amikacin
conjugation
synergism
antimicrobial activity
hemolytic activity

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description: 83 pages, 24 pictures, 6 tables. Theme of the final qualification work: "A comparative analysis of the biological activity of the antimicrobial peptide, its conjugate with amikacin and combinations with silver nanoparticles". This work is devoted to the study of the antimicrobial and hemolytic properties of a peptide having a Î²-hairpin conformation, its conjugate with amikacin and combinations with silver nanoparticles. As a result of the work, the minimum inhibitory concentrations of the studied substances against bacteria were determined by the method of serial dilutions in liquid nutrient media containing microorganisms. Synergistic and additive effects have been identified with the combined action of a peptide with silver nanoparticles using serial dilutions using the checkerboard method. The effect of the peptide and its combinations with silver nanoparticles on the permeability of the inner membrane of E. coli ML-35p is compared by the method of photometric assessment of membrane permeability for chromogenic markers. Assessed the toxicity of the studied substances in relation to human red blood cells using a hemolytic test. It was found that the studied substances have pronounced antimicrobial activity and significantly lower hemolytic activity than the native peptide and therefore can be considered as promising prototypes of new drugs for the treatment of infections caused by antibiotic-resistant bacteria. This work was carried out on the basis of the laboratory for the design and synthesis of biologically active peptides of the Department of General Pathology and Pathological Physiology of the Institute of Experimental Medicine.