title: ÐÐ·ÑÑÐµÐ½Ð¸Ðµ Ð¼ÐµÑ Ð°Ð½Ð¸Ð·Ð¼Ð° Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð² Ð¿ÑÐ¸ÑÐ¾Ð´Ð½Ð¾Ð³Ð¾ Ð¿ÑÐ¾Ð¸ÑÑ Ð¾Ð¶Ð´ÐµÐ½Ð¸Ñ Ð² Ð¾ÑÐ½Ð¾ÑÐµÐ½Ð¸Ð¸ ÑÐµÑÐ¿Ð¸ÑÐ°ÑÐ¾ÑÐ½Ð¾-ÑÐ¸Ð½ÑÐ¸ÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð²Ð¸ÑÑÑÐ°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Study of the mechanism of action of drugs of natural origin in relation to respiratory syncytial virus
Creators: name: ÐÐ»Ð°Ð±ÑÐºÐ¾Ð², ÐÑÑÑÐ¼
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: ÑÐµÑÐ¿Ð¸ÑÐ°ÑÐ¾ÑÐ½Ð¾-ÑÐ¸Ð½ÑÐ¸ÑÐ¸Ð°Ð»ÑÐ½ÑÐ¹ Ð²Ð¸ÑÑÑ
Ñ Ð¸Ð¼Ð¸Ð¾ÑÐµÑÐ°Ð¿Ð¸Ñ Ð²Ð¸ÑÑÑÐ½ÑÑ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¹
ÑÑÑÐ¾Ð¹ÑÐ¸Ð²ÑÐ¹ ÑÑÐ°Ð¼Ð¼
ÐºÐ¾Ð½ÑÐ¾ÐºÐ°Ð»ÑÐ½Ð°Ñ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ
Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ Ð¿ÑÐ¾ÑÐ¸Ð²Ð¾Ð²Ð¸ÑÑÑÐ½Ð¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ in vivo
ÐºÑÐ¼Ð°ÑÐ¸Ð½Ñ
respiratory syncythial virus
chemotherapy for viral infections
resistant strain
confocal microscopy
studying the antiviral activity in vivo
coumarins

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð¼ÐµÑÐ°Ð½Ð¸Ð·Ð¼Ð° Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ Ð¿ÑÐ¾ÑÐ¸Ð²Ð¾Ð²Ð¸ÑÑÑÐ½Ð¾Ð³Ð¾ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ° K142. Ð ÐµÑÐ¿Ð¸ÑÐ°ÑÐ¾ÑÐ½Ð¾-ÑÐ¸Ð½ÑÐ¸ÑÐ¸Ð°Ð»ÑÐ½ÑÐ¹ Ð²Ð¸ÑÑÑ Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÑÐµÑ ÑÐµÑÑÐµÐ·Ð½ÑÑ ÑÐ³ÑÐ¾Ð·Ñ Ð´Ð»Ñ ÑÑÐ·Ð²Ð¸Ð¼ÑÑ Ð³ÑÑÐ¿Ð¿ Ð»ÑÐ´ÐµÐ¹, ÑÐ°ÐºÐ¸Ñ ÐºÐ°Ðº Ð¼Ð»Ð°Ð´ÐµÐ½ÑÑ Ð¸ Ð¿Ð¾Ð¶Ð¸Ð»ÑÐµ Ð»ÑÐ´Ð¸. Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð½Ð¾Ð²ÑÑ ÑÑÐ¸Ð¾ÑÑÐ¾Ð¿Ð½ÑÑ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð»ÐµÑÐµÐ½Ð¸Ñ ÑÐµÑÐ¿Ð¸ÑÐ°ÑÐ¾ÑÐ½Ð¾-ÑÐ¸Ð½ÑÐ¸ÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð²Ð¸ÑÑÑÐ° ÐºÑÐ°Ð¹Ð½Ðµ Ð°ÐºÑÑÐ°Ð»ÑÐ½Ð°, ÑÐ°Ðº ÐºÐ°Ðº Ð½Ðµ ÑÑÑÐµÑÑÐ²ÑÐµÑ ÑÐ½Ð¸Ð²ÐµÑÑÐ°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð¼ÐµÑÐ¾Ð´Ð° Ð±Ð¾ÑÑÐ±Ñ Ñ Ð¸Ð½ÑÐµÐºÑÐ¸ÐµÐ¹. Ð Ð´Ð°Ð½Ð½Ð¾Ð¼ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ð¸ Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»Ð°ÑÑ Ð¾ÑÐµÐ½ÐºÐ° Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ° Ð½Ð° ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÐµ ÑÑÐ°Ð´Ð¸Ð¸ Ð²Ð¸ÑÑÑÐ½Ð¾Ð³Ð¾ ÑÐ¸ÐºÐ»Ð° in vitro Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ ÐºÐ¾Ð½ÑÐ¾ÐºÐ°Ð»ÑÐ½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸, Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»ÑÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½Ñ Ñ ÑÐµÐ»ÑÑ Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾Ð³Ð¾ ÑÑÐ°Ð¼Ð¼Ð° ÑÐµÑÐ¿Ð¸ÑÐ°ÑÐ¾ÑÐ½Ð¾-ÑÐ¸Ð½ÑÐ¸ÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð²Ð¸ÑÑÑÐ°, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¾ÑÐµÐ½ÐºÐ° Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ° in vivo Ð½Ð° Ð¼Ð¾Ð´ÐµÐ»Ð¸ ÑÐµÑÐ¿Ð¸ÑÐ°ÑÐ¾ÑÐ½Ð¾-ÑÐ¸Ð½ÑÐ¸ÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð¹ Ð¸Ð½ÑÐµÐºÑÐ¸Ð¸ Ñ Ð»Ð¸Ð½ÐµÐ¹Ð½ÑÑ Ð¼ÑÑÐµÐ¹. ÐÐ°Ð½Ð½ÑÐµ, Ð¿Ð¾Ð»ÑÑÐµÐ½Ð½ÑÐµ Ñ Ð¿Ð¾Ð¼Ð¾ÑÑÑ ÐºÐ¾Ð½ÑÐ¾ÐºÐ°Ð»ÑÐ½Ð¾Ð¹ Ð¼Ð¸ÐºÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ð¸, Ð¿Ð¾Ð´ÑÐ²ÐµÑÐ¶Ð´Ð°ÑÑ Ð¿ÑÐµÐ´Ð¿Ð¾Ð»Ð¾Ð¶ÐµÐ½Ð¸Ñ, Ð¸Ð·Ð»Ð¾Ð¶ÐµÐ½Ð½ÑÐµ Ð² Ð¿ÑÐµÐ´ÑÐ´ÑÑÐ¸Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸ÑÑ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ° Ð142 Ð¾ ÐµÐ³Ð¾ Ð¼Ð¸ÑÐµÐ½Ð¸ â Ð¿Ð¾Ð²ÐµÑÑÐ½Ð¾ÑÑÐ½Ð¾Ð¼ F Ð±ÐµÐ»ÐºÐµ ÑÐµÑÐ¿Ð¸ÑÐ°ÑÐ¾ÑÐ½Ð¾-ÑÐ¸Ð½ÑÐ¸ÑÐ¸Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð²Ð¸ÑÑÑÐ°. Ð¨ÑÐ°Ð¼Ð¼ Ð²Ð¸ÑÑÑÐ°, Ð¿Ð°ÑÑÐ¸ÑÐ¾Ð²Ð°Ð²ÑÐ¸Ð¹ÑÑ Ð² Ð¿ÑÐ¸ÑÑÑÑÑÐ²Ð¸Ð¸ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ° Ð½Ðµ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð» Ð´Ð¾ÑÑÐ¾Ð²ÐµÑÐ½Ð¾Ð¹ ÑÑÑÐ¾Ð¹ÑÐ¸Ð²Ð¾ÑÑÐ¸ Ðº ÐµÐ³Ð¾ Ð´ÐµÐ¹ÑÑÐ²Ð¸Ñ. Ð ÐµÐ·ÑÐ»ÑÑÐ°ÑÑ Ð¾Ð¿ÑÑÐ¾Ð² in vivo Ð¿Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÑÑ ÑÐ»Ð°Ð±Ð¾Ðµ Ð¿ÑÐ¾ÑÐ¸Ð²Ð¾Ð²Ð¸ÑÑÑÐ½ÑÑ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð² Ð³ÑÑÐ¿Ð¿Ðµ, Ð¿Ð¾Ð»ÑÑÐ°Ð²ÑÐµÐ¹ Ð¿ÑÐµÐ¿Ð°ÑÐ°Ñ Ð¸Ð½ÑÑÐ°Ð½Ð°Ð·Ð°Ð»ÑÐ½Ð¾, ÑÑÐ¾ Ð¼Ð¾Ð¶ÐµÑ Ð³Ð¾Ð²Ð¾ÑÐ¸ÑÑ Ð¾ Ð½ÐµÐ´Ð¾ÑÑÐ°ÑÐ¾ÑÐ½Ð¾ ÑÐ¾ÑÐ¾ÑÐµÐ¹ Ð±Ð¸Ð¾Ð´Ð¾ÑÑÑÐ¿Ð½Ð¾ÑÑÐ¸ Ð´Ð°Ð½Ð½Ð¾Ð¹ Ð¼Ð¾Ð»ÐµÐºÑÐ»Ñ. ÐÐ°Ð»ÑÐ½ÐµÐ¹ÑÐµÐµ ÑÑÐ¾Ð²ÐµÑÑÐµÐ½ÑÑÐ²Ð¾Ð²Ð°Ð½Ð¸Ðµ ÑÐ¾ÑÐ¼ÑÐ»Ñ Ð¼Ð¾Ð¶ÐµÑ ÑÐ²ÐµÐ»Ð¸ÑÐ¸ÑÑ ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ° in vivo Ð¸ Ð²ÑÐ²ÐµÑÑÐ¸ ÐµÐ³Ð¾ Ð½Ð° ÑÐ»ÐµÐ´ÑÑÑÑÑ ÑÑÐ°Ð´Ð¸Ñ Ð´Ð¾ÐºÐ»Ð¸Ð½Ð¸ÑÐµÑÐºÐ¸Ñ Ð¸ÑÐ¿ÑÑÐ°Ð½Ð¸Ð¹.
description: This work is devoted to studying the mechanism of action of the antiviral drug K142. Respiratory syncytial virus poses a serious threat to vulnerable groups of people such as infants and the elderly. The development of new etiotropic methods for the treatment of respiratory syncytial virus is extremely relevant since there is no universal method to fight infection. In this study, the effect of the drug on various stages of the viral cycle in vitro was assessed by confocal microscopy, an experiment was conducted to obtain a resistant strain of respiratory syncytial virus, and the activity of the drug in vivo was evaluated in a model of respiratory syncytial infection in linear mice. The data obtained using confocal microscopy confirm the assumptions made in previous studies of the K142 preparation about its target, the surface F protein of the respiratory syncytial virus. The virus strain passaged in the presence of the drug did not show significant resistance to it. The results of in vivo experiments show a weak antiviral activity in the group that received the drug intranasally, this may indicate that the bioavailability of this molecule is not good enough. Further modification of the formula may increase the in vivo efficacy of the drug and bring it to the next stage of preclinical trial.