cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
title: Информационное право
Creators: name: ELS IPR BOOKS
Publisher: name: Профобразование
Year: 2024

description: В учебном пособии рассмотрены теоретические основы информационного права, информационной безопасности. Приведены объекты, субъекты и методы правового регулирования общественных отношений в сфере информационного права РФ, основы правовой защиты конфиденциальной информации, классификация институтов правовой защиты, перспективы развития законодательства в области информационной безопасности РФ. Учебное пособие предназначено для студентов укрупненных групп специальностей среднего профессионального образования «Информатика и вычислительная техника», «Информационная безопасность», «Электроника, радиотехника и системы связи», учебными планами которых предусмотрено изучение дисциплины «Информационное право».
Safety and biodistribution assessment of sc-rAAV2.5IL-1Ra administered via intra-articular injection in a mono-iodoacetate-induced osteoarthritis rat model
Creators: name: Terse, Pramod S
name: Evans, Christopher H
name: Wilder, Julie A
name: Seagrave, JeanClare
name: Benson, Janet M
name: Hutt, Julie A
name: Grieger, Joshua C
name: Samulski, R Jude
name: Wang, Gensheng
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2016

description: Interleukin-1 (IL-1) plays an important role in the pathophysiology of osteoarthritis (OA), and gene transfer of IL-1 receptor antagonist (IL-1Ra) holds promise for OA treatment. A preclinical safety and biodistribution study evaluated a self-complementary adeno-associated viral vector carrying rat IL-1Ra transgene (sc-rAAV2.5rIL-1Ra) at 5 × 108, 5 × 109, or 5 × 1010 vg/knee, or human IL-1Ra transgene (sc-rAAV2.5hIL-1Ra) at 5 × 1010 vg/knee, in Wistar rats with mono-iodoacetate (MIA)–induced OA at days 7, 26, 91, 180, and 364 following intra-articular injection. The MIA-induced OA lesions were consistent with the published data on this model. The vector genomes persisted in the injected knees for up to a year with only limited vector leakage to systemic circulation and uptake in tissues outside the knee. Low levels of IL-1Ra expression and mitigation of OA lesions were observed in the vector-injected knees, albeit inconsistently. Neutralizing antibodies against the vector capsid developed in a dose-dependent manner, but only the human vector induced a small splenic T-cell immune response to the vector capsid. No local or systemic toxicity attributable to vector administration was identified in the rats as indicated by clinical signs, body weight, feed consumption, clinical pathology, and gross and microscopic pathology through day 364. Taken together, the gene therapy vector demonstrated a favorable safety profile.
title: Die „Pull-Back“ Technik für die 532 Slim Modiolar Electrode
Creators: name: Riemann, Conrad
name: Todt, Ingo
name: Sudhoff, Holger
Publisher: name: German Medical Science GMS Publishing House
Year: 2019
Subjects: subject: 610 Medical sciences; Medicine

description: Einleitung: Der Abstand zwischen dem Modiolus und dem Elektrodenarray ist ein Faktor, der im Mittelpunkt vieler Diskussionen und Studien steht. Das Einbringen des Elektrodenarrays näher zum Spiralganglion, mit dem Ziel die Stromausbreitung zu reduzieren, konnte bessere Hörergebnisse demonstrieren.[zum vollständigen Text gelangen Sie über die oben angegebene URL]
description: Jahrestagung der Vereinigung Westdeutscher Hals-Nasen-Ohren-Ärzte
Underestimated role of nodulated roots for soil fertility and quality
Creators: name: Carranca, Corina
name: Madeira, Manuel
Publisher: name: Zenodo
Year: 2022
Subjects: Aboveground tissue
improved permanent pasture
Mediterranean region
15N technique
recovered root and nodule
sweet and yellow lupines

description: Nitrogen (N) storage in legumes is usually estimated by N2 fixation in shoots, whereas there is little knowledge on the contribution of roots and nodules to legume N and soil N. Here, we studied the contribution of recovered roots and nodules of grain and pasture legumes to plant N and soil N in Mediterranean fields. Experiments were run under rainfed conditions for a 2-yr period in three regions of Portugal (central and south west, and south inland). Complete plants including top plant and visible roots and nodules were sampled at the end of the growing seasons for grain legumes, sweet and yellow lupines, and over two harvests in case of pastures. N2 fixation was measured by the 15N techniques. Results showed that aboveground N concentration did not vary significantly among species, but differed in the belowground tissues (Carranca et al., 2015). Roots and nodules contributed to 7 – 11% of total legume N, with an allocation of 11 – 14 kg N fixed t−1 belowground dry weight (DW) in indeterminate legumes, representing half amount of aboveground plant. This finding demonstrated that investigation relying only on shoot N underestimates the role of legumes for soil N fertility. Further long - term studies on the contribution of belowground tissues to the soil N fertility are fully encouraged. Rhizodeposits also contribute strongly for stable organic matter in soils improving the soil structure (Pinto et al., 2021). Caddish et al. (2002) reinforced this statement and referred that nodulated roots and rhizodeposits may be protected from mineralization then playing a more important role in building of soil structure rather than in soil N supply by its high C concentration.
Asplenium inaequilaterale Willd., Sp. Pl.
Creators: name: Kessler, Michael
name: Smith, Alan R.
Publisher: name: Zenodo
Year: 2018
Subjects: Biodiversity
Taxonomy
Plantae
Tracheophyta
Polypodiopsida
Polypodiales
Aspleniaceae
Asplenium
Asplenium inaequilaterale

description: Asplenium inaequilaterale Willd., Sp. Pl. 5(1): 322. 1810.
 Range:— Bolivia (CH, CO, LP, SC, TA), Argentina; southeastern Brazil; Africa; Madagascar; Mascarenes; southern India, Sri Lanka.
 Ecology:— Fairly common; terrestrial and saxicolous in semihumid forests; 950–2400 m.
 Notes:— The distinctness of this species from A. discrepans is uncertain, but pinnae of A. inaequilaterale are generally larger, 3–5 cm long, as well as wider (usually> 1 cm); hybrids are suspected from Bolivia (Sundue 2011).
description: Published as part of Kessler, Michael & Smith, Alan R., 2018, Prodromus of a fern flora for Bolivia. XXIX. Aspleniaceae, pp. 259-280 in Phytotaxa 344 (3) on pages 259-280, DOI: 10.11646/phytotaxa.344.3.6, http://zenodo.org/record/13720625
Asplenium lilloanum de la Sota, Darwiniana
Creators: name: Kessler, Michael
name: Smith, Alan R.
Publisher: name: Zenodo
Year: 2018
Subjects: Biodiversity
Taxonomy
Plantae
Tracheophyta
Polypodiopsida
Polypodiales
Aspleniaceae
Asplenium
Asplenium lilloanum

description: Asplenium lilloanum de la Sota, Darwiniana 18: 198. 1973.
 Range:— Bolivia (SC, TA) to northwestern Argentina.
 Ecology:— Uncommon; saxicolous, rarely terrestrial, on rocks, ledges, and in crevices of rock faces, in semihumid forests; 1700–2150 m.
 Notes:— Young leaves of this species, A. palmeri, and A. resiliens have ill-developed blade apices lacking the shapes that are characteristic of the respective species, which may lead to difficulties in identification.
description: Published as part of Kessler, Michael & Smith, Alan R., 2018, Prodromus of a fern flora for Bolivia. XXIX. Aspleniaceae, pp. 259-280 in Phytotaxa 344 (3) on pages 259-280, DOI: 10.11646/phytotaxa.344.3.6, http://zenodo.org/record/13720625
title: Основы компьютерного проектирования и моделирования РЭС
Creators: name: ELS IPR BOOKS
Publisher: name: Профобразование
Year: 2024

description: В практикуме представлены шесть лабораторных работ. В первой и во второй лабораторных работах осуществляется исследование цепей на постоянном токе и во временной области на моделях в среде MULTISIM. Последующие две лабораторные работы посвящены анализу электрических цепей, характеристик усилителя на моделях в среде MULTISIM. В пятой и шестой лабораторных работах проводится моделирование компрессора и стабилизатора напряжения. Практикум может быть использован студентами укрупненных групп специальностей среднего профессионального образования «Информационная безопасность», «Электроника, радиотехника и системы связи» при изучении дисциплин «Схемотехника», «Цифровая схемотехника».
title: Tracheopathia osteochondroplastica des Larynx – ein Fallbericht
Creators: name: Stein, Christine
name: Minovi, Amir
Publisher: name: German Medical Science GMS Publishing House
Year: 2019
Subjects: subject: 610 Medical sciences; Medicine

description: Einleitung: Die Tracheopathia osteochondroplastica (TO) ist eine äußerst seltene Erkrankung, die sich durch knöcherne und knorpelige submukosale Knötchen der Trachea und Hauptbronchien charakterisiert. Eine selektive Manifestation im Bereich des Larynx wurde bis jetzt nur in wenigen[zum vollständigen Text gelangen Sie über die oben angegebene URL]
description: Jahrestagung der Vereinigung Westdeutscher Hals-Nasen-Ohren-Ärzte
Asplenium palmeri Maxon, Contr. U. S. Natl. Herb.
Creators: name: Kessler, Michael
name: Smith, Alan R.
Publisher: name: Zenodo
Year: 2018
Subjects: Biodiversity
Taxonomy
Plantae
Tracheophyta
Polypodiopsida
Polypodiales
Aspleniaceae
Asplenium
Asplenium palmeri

description: Asplenium palmeri Maxon, Contr. U.S. Natl. Herb. 13: 39. 1909.
 Range:— Southwestern USA to Colombia and western Venezuela; Ecuador; Peru; Bolivia (CH, CO, LP, SC) to northwestern Argentina.
 Ecology:— Locally fairly common; terrestrial in semideciduous to deciduous forests; 1200–2550 m.
 Notes:— Part of the A. monanthes complex (Dyer et al. 2012).
description: Published as part of Kessler, Michael & Smith, Alan R., 2018, Prodromus of a fern flora for Bolivia. XXIX. Aspleniaceae, pp. 259-280 in Phytotaxa 344 (3) on pages 259-280, DOI: 10.11646/phytotaxa.344.3.6, http://zenodo.org/record/13720625
2D ice from first principles: structures and phase transitions
Creators: name: Chen, Ji
name: Schusteritsch, Georg
name: Pickard, Chris J.
name: Salzmann, Christoph G.
name: Michaelides, Angelos
Publisher: name: arXiv
Year: 2015
Subjects: subject: Materials Science (cond-mat.mtrl-sci)
subject: FOS: Physical sciences

description: Despite relevance to disparate areas such as cloud microphysics and tribology, major gaps in the understanding of the structures and phase transitions of low-dimensional water ice remain. Here we report a first principles study of confined 2D ice as a function of pressure. We find that at ambient pressure hexagonal and pentagonal monolayer structures are the two lowest enthalpy phases identified. Upon mild compression the pentagonal structure becomes the most stable and persists up to \textit{ca.} 2 GPa at which point square and rhombic phases are stable. The square phase agrees with recent experimental observations of square ice confined within graphene sheets. We also find a double layer AA stacked square ice phase, which clarifies the difference between experimental observations and earlier force field simulations. This work provides a fresh perspective on 2D confined ice, highlighting the sensitivity of the structures observed to both the confining pressure and width.
title: High prevalence rates of Toxoplasma gondii in cat-hunted small mammals – Evidence for parasite induced behavioural manipulation in the natural environment?
Creators: name: Pardo Gil, Miguel
Publisher: name: Universität Bern
Year: 2023
Subjects: subject: 590 Animals (Zoology)
subject: 610 Medicine & health

description: Toxoplasma gondii causes one of the most frequent parasitic infections in vertebrates on earth. The present study aimed to assess the occurrence of T. gondii infection in cat-hunted wild small mammals, and to determine the circulating T. gondii genotypes in cat prey. There is evidence suggesting that T. gondii may manipulate rodents’ behaviour enhancing transmission to their definitive feline host by facilitating predation. Given that most studies focusing on rodent behavior have been performed under laboratory conditions, we tested this hypothesis in the natural environment. We analysed 157 cat-hunted wild small mammals of six different species from Switzerland. Brain and skeletal muscle samples from each animal were tested for T. gondii DNA by PCR, and positive samples were genotyped using a multilocus sequence typing approach. The prevalence of T. gondii in cat-hunted Arvicola amphibius s.l. was 11.1%, 14.6% in Apodemus spp., 13.6% in Myodes glareolus, 6.7% in Crocidura russula, and 0% in Microtus arvalis and Sorex sp. All completely genotyped T. gondii parasites, exhibited the ToxoDB #3 genotype, a Type II variant. We additionally analysed 48 trap-captured A. amphibius s.l., which all tested negative for T. gondii infection, contrasting with the higher prevalence in cat-hunted A. amphibius s.l. These results provide evidence that T. gondii infected rodents are at higher risk to be predated by cats and therewith support the behaviour manipulation hypothesis.
title: Основы теории радиолокации
Creators: name: ELS IPR BOOKS
Publisher: name: Профобразование
Year: 2024

description: В учебном пособии приведены теоретические основы радиолокационных систем, включая обнаружение сигналов, уравнение радиолокации, выбор зондирующих сигналов и их анализ. Описаны характеристики пассивных помех, алгоритмы уменьшения влияния пассивных помех. Учебное пособие предназначено для студентов, обучающихся по специальности среднего профессионального образования «Радиотехнические информационные системы», изучающих дисциплину «Основы радиотехники и радиолокации».
title: Основы теории радиолокационных систем и комплексов
Creators: name: ELS IPR BOOKS
Publisher: name: Профобразование
Year: 2024

description: В учебном пособии изложены основы теории радиолокационных систем, включая теорию решающих устройств, теорию потенциальной помехоустойчивости сигналов, основы теории статистических решений. Приведены основы выбора зондирующего сигнала в радиолокационных системах, характеристики активных и пассивных помех, а также алгоритмы их уменьшения. Учебное пособие предназначено для студентов, обучающихся по специальности среднего профессионального образования «Радиотехнические информационные системы», изучающих дисциплину «Основы радиотехники и радиолокации».
Development of a Novel AAV Gene Therapy Cassette with Improved Safety Features and Efficacy in a Mouse Model of Rett Syndrome
Creators: name: Gray, Steven J.
name: Hector, Ralph D.
name: Bailey, Mark E.S.
name: Gadalla, Kamal K.E.
name: Bahey, Noha G.
name: Cobb, Stuart R.
name: Vudhironarit, Thishnapha
name: Sinnett, Sarah
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2017

description: Rett syndrome (RTT), caused by loss-of-function mutations in the MECP2 gene, is a neurological disorder characterized by severe impairment of motor and cognitive functions. The aim of this study was to investigate the impact of vector design, dosage and delivery route on the efficacy and safety of gene augmentation therapy in mouse models of RTT. Our results show that AAV-mediated delivery of MECP2 to Mecp2-null mice by systemic administration, and utilizing a minimal endogenous promoter, was associated with a narrow therapeutic window and resulted in liver toxicity at higher doses. Lower doses of this vector significantly extended survival of mice lacking MeCP2 or expressing a mutant T158M allele but had no impact on RTT-like neurological phenotypes. Modifying vector design by incorporating an extended Mecp2 promoter and additional regulatory 3’-UTR elements significantly reduced hepatic toxicity after systemic administration. Moreover, direct cerebroventricular injection of this vector into neonatal Mecp2-null mice resulted in high brain transduction efficiency, increased survival and bodyweight and an amelioration of RTT-like phenotypes. Our results show that controlling levels of MeCP2 expression in the liver is achievable through modification of the expression cassette. However, it also highlights the importance of achieving high brain transduction to impact the RTT-like phenotypes.
title: Einfluss auf die subjektive Vertikale durch Cochlea-Implantate nach Cochleosacculotomie bei Morbus Menière
Creators: name: Ilgner, Justus
name: Duong Dinh, Thien An
name: Westhofen, Martin
Publisher: name: German Medical Science GMS Publishing House
Year: 2019
Subjects: subject: 610 Medical sciences; Medicine

description: Einleitung: Bei Patienten mit fortgeschrittenen Stadien eines Morbus Menière besteht neben residualen Drehschwindelanfällen wegen gestörter labyrinthärer Restfunktion häufig ein ausgeprägter sensorischer Hörverlust, der ein Sprachverstehen auch unter Hörgeräte-Versorgung[zum vollständigen Text gelangen Sie über die oben angegebene URL]
description: Jahrestagung der Vereinigung Westdeutscher Hals-Nasen-Ohren-Ärzte
Efficacy of Gene Therapy Is Dependent on Disease Progression in Dystrophic Mice with Mutations in the FKRP Gene
Creators: name: Xiao, Will
name: Vannoy, Charles Harvey
name: Xiao, Xiao
name: Lu, Qi Long
name: Lu, Peijuan
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2017

description: Loss-of-function mutations in the Fukutin-related protein ( ) gene cause limb-girdle muscular dystrophy type 2I (LGMD2I) and other forms of congenital muscular dystrophy-dystroglycanopathy that are associated with glycosylation defects in the α-dystroglycan (α-DG) protein. Systemic administration of a single dose of recombinant adeno-associated virus serotype 9 (AAV9) vector expressing human to a mouse model of LGMD2I at various stages of disease progression was evaluated. The results demonstrate rescue of functional glycosylation of α-DG and muscle function, along with improvements in muscle structure at all disease stages versus age-matched untreated cohorts. Nevertheless, mice treated in the latter stages of disease progression revealed a decrease in beneficial effects of the treatment. The results provide a proof of concept for future clinical trials in patients with -related muscular dystrophy and demonstrate that AAV-mediated gene therapy can potentially benefit patients at all stages of disease progression, but earlier intervention would be highly preferred.
Transfer technologií a open science: kamarádi nebo rivalové?
Creators: name: Machů, Matěj
name: Marek, Jiří
name: Woznica, Ondřej
Publisher: name: Zenodo
Year: 2022

description: Tato prezentace byla použita tvořila základ pro přednášku a panelovou diskuzi v rámci Open Science Festu 2022, který se konal dne 25.10.2022.
title: Основы физической оптики
Creators: name: ELS IPR BOOKS
Publisher: name: Профобразование
Year: 2024

description: В практикуме представлены практические работы, в которых рассматриваются законы отражения и преломления света, явления интерференции, дифракции света на двумерных структурах и на периодической решетке, явления внешнего фотоэффекта. Практикум предназначен для студентов укрупненной группы специальностей среднего профессионального образования «Электроника, радиотехника и системы связи», учебными планами которых предусмотрено изучение дисциплин «Физика оптики», «Основы оптики и фотоники». Издание также может быть полезно студентам укрупненной группы «Фотоника, приборостроение, оптические и биотехнические системы и технологии».
Asplenium lorentzii Hieron., Bot. Jahrb. Syst.
Creators: name: Kessler, Michael
name: Smith, Alan R.
Publisher: name: Zenodo
Year: 2018
Subjects: Biodiversity
Taxonomy
Plantae
Tracheophyta
Polypodiopsida
Polypodiales
Aspleniaceae
Asplenium
Asplenium lorentzii

description: Asplenium lorentzii Hieron., Bot. Jahrb. Syst. 22: 375. 1896.
 Range:— Peru, Bolivia (CH, LP, SC, TA), and Argentina.
 Ecology:— Fairly common; saxicolous or terrestrial in semihumid forests, especially of Alnus Mill. and Podocarpus L’Hér. ex Pers.; 1500–3400 m; in Peru also in coastal fog vegetation at 700 m (León et al. 2002). Often forms dense clonal populations.
 Notes:— This species, along with A. gilliesii and A. peruvianum, reproduces vegetatively by scaly proliferous buds. The buds are located at the blade bases on fully developed leaves, or at the apex of an elongate petiole. In the latter case, development of the blade is delayed, giving the petiole the appearance of a stolon.
description: Published as part of Kessler, Michael & Smith, Alan R., 2018, Prodromus of a fern flora for Bolivia. XXIX. Aspleniaceae, pp. 259-280 in Phytotaxa 344 (3) on pages 259-280, DOI: 10.11646/phytotaxa.344.3.6, http://zenodo.org/record/13720625
Institutional change and organisational resistance to gender equality in higher education: an Irish case study
Creators: name: Hodgins, Margaret
name: O'CONNOR, PAT
name: Buckley, Lucy-Ann
Publisher: name: University of Limerick
Year: 2023
Subjects: subject: Sociology
subject: FOS: Sociology

description: Attempts to transform the gendered structures and cultures of higher education institutions have had limited success. This article focuses on one Irish university (pseudonym University A) where gender inequality was a major concern culminating in high-profile litigation. Using a feminist institutional approach, it asks: (1) What changes and interventions were introduced in the context of a favorable national policy environment and local grassroots support? and (2) how were these interventions perceived by staff? The methodology draws, firstly on the authors’ personal knowledge of the local context; secondly, on an analysis of University A’s key gender equality-related actions and documents; and thirdly, on a thematic analysis of qualitative data from 129 respondents in an online survey. Positive changes are identified, as is institutional resistance, reflected in low ambitions and focus on individualistic solutions. In the qualitative data, resistance involving denial; assertions that the problem is solved; the importance of meritocracy, and a focus on “fixing the women” (and the men) were identified. Acceptance of gender equality as an organisational issue was reflected in criticism of the interventions as tokenistic window dressing; not impacting on the culture and “not going far enough”. The implications for effectively addressing gender inequality are discussed.
Toward Personalized Gene Therapy: Characterizing the Host Genetic Control of Lentiviral-Vector-Mediated Hepatic Gene Delivery
Creators: name: Montgomery, Stephanie A.
name: Pardo-Manuel de Villena, Fernando
name: Gui, Tong
name: Hu, Peirong
name: Suwanmanee, Thipparat
name: Ferris, Martin T.
name: Kafri, Tal
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2017

description: The success of lentiviral vectors in curing fatal genetic and acquired diseases has opened a new era in human gene therapy. However, variability in the efficacy and safety of this therapeutic approach has been reported in human patients. Consequently, lentiviral-vector-based gene therapy is limited to incurable human diseases, with little understanding of the underlying causes of adverse effects and poor efficacy. To assess the role that host genetic variation has on efficacy of gene therapy, we characterized lentiviral-vector gene therapy within a set of 12 collaborative cross mouse strains. Lentiviral vectors carrying the firefly luciferase cDNA under the control of a liver-specific promoter were administered to female mice, with total-body and hepatic luciferase expression periodically monitored through 41 weeks post-vector administration. Vector copy number per diploid genome in mouse liver and spleen was determined at the end of this study. We identified major strain-specific contributions to overall success of transduction, vector biodistribution, maximum luciferase expression, and the kinetics of luciferase expression throughout the study. Our results highlight the importance of genetic variation on gene-therapeutic efficacy; provide new models with which to more rigorously assess gene therapy approaches; and suggest that redesigning preclinical studies of gene-therapy methodologies might be appropriate.
Apicoplast ribosomal protein S10-V127M enhances artemisinin resistance of a Kelch13 transgenic Plasmodium falciparum.
Creators: name: Kampoun, Tanyaluck
name: Srichairatanakool, Somdet
name: Prommana, Parichat
name: Shaw, Philip J
name: Green, Judith L
name: Knuepfer, Ellen
name: Holder, Anthony A
name: Uthaipibull, Chairat
Publisher: name: The Francis Crick Institute
Year: 2022
Subjects: Model organisms
Biochemistry & Proteomics
Infectious Disease
Cell Biology

description: BACKGROUND: The resistance of Plasmodium falciparum to artemisinin-based (ART) drugs, the front-line drug family used in artemisinin-based combination therapy (ACT) for treatment of malaria, is of great concern. Mutations in the kelch13 (k13) gene (for example, those resulting in the Cys580Tyr [C580Y] variant) were identified as genetic markers for ART-resistant parasites, which suggests they are associated with resistance mechanisms. However, not all resistant parasites contain a k13 mutation, and clearly greater understanding of resistance mechanisms is required. A genome-wide association study (GWAS) found single nucleotide polymorphisms associated with ART-resistance in fd (ferredoxin), arps10 (apicoplast ribosomal protein S10), mdr2 (multidrug resistance protein 2), and crt (chloroquine resistance transporter), in addition to k13 gene mutations, suggesting that these alleles contribute to the resistance phenotype. The importance of the FD and ARPS10 variants in ART resistance was then studied since both proteins likely function in the apicoplast, which is a location distinct from that of K13. METHODS: The reported mutations were introduced, together with a mutation to produce the k13-C580Y variant into the ART-sensitive 3D7 parasite line and the effect on ART-susceptibility using the 0-3 h ring survival assay (RSA0-3 h) was investigated. RESULTS AND CONCLUSION: Introducing both fd-D193Y and arps10-V127M into a k13-C580Y-containing parasite, but not a wild-type k13 parasite, increased survival of the parasite in the RSA0-3 h. The results suggest epistasis of arps10 and k13, with arps10-V127M a modifier of ART susceptibility in different k13 allele backgrounds.
title: Time-to-event latent variable models for the statistical analysis of clinical data
Creators: name: Lythgoe, Daniel
Publisher: name: University of Liverpool Repository
Year: 2020

description: In clinical research, interest sometimes lies in analysing variables which are not measured directly. Instead, information about these ‘latent variables’ can be inferred from surrogates or other imperfect indicators, using latent variable models. Common examples of ‘hypothetical’ latent variables in clinical research include quality of life (QoL), anxiety and depression. Another type of latent variable is a variable used as a device for dimension reduction, for example, a principal component. The aim of this thesis is to explore and develop latent variable methods for the statistical analysis of clinical data, with an emphasis on including latent variables in time-to-event models.
CNS-restricted Transduction and CRISPR/Cas9-mediated Gene Deletion with an Engineered AAV Vector
Creators: name: Murlidharan, Giridhar
name: Corriher, Travis
name: Sakamoto, Kensuke
name: Rao, Lavanya
name: Asokan, Aravind
name: Wang, Dan
name: Sullivan, Patrick
name: Gao, Guangping
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2016

description: Gene therapy using recombinant adeno-associated viral (AAV) vectors is emerging as a promising approach to treat central nervous system disorders such as Spinal muscular atrophy, Batten, Parkinson and Alzheimer disease amongst others. A critical remaining challenge for central nervous system-targeted gene therapy, silencing or gene editing is to limit potential vector dose-related toxicity in off-target cells and organs. Here, we characterize a lab-derived AAV chimeric (AAV2g9), which displays favorable central nervous system attributes derived from both parental counterparts, AAV2 and AAV9. This synthetic AAV strain displays preferential, robust, and widespread neuronal transduction within the brain and decreased glial tropism. Importantly, we observed minimal systemic leakage, decreased sequestration and gene transfer in off-target organs with AAV2g9, when administered into the cerebrospinal fluid. A single intracranial injection of AAV2g9 vectors encoding guide RNAs targeting the schizophrenia risk gene MIR137 (encoding MIR137) in CRISPR/Cas9 knockin mice resulted in brain-specific gene deletion with no detectable events in the liver. This engineered AAV vector is a promising platform for treating neurological disorders through gene therapy, silencing or editing modalities.
title: Правовое обеспечение связей с общественностью
Creators: name: ELS IPR BOOKS
Publisher: name: Профобразование
Year: 2024

description: В учебном пособии рассмотрены правовые основы регулирования информационной сферы, в частности средств массовой информации, рекламы и журналистики. Представлены правовые и этические требования к производству и размещению информационно-рекламных продуктов, базовые положения законодательства Российской Федерации в области интеллектуальной собственности и регулирования трудовых и гражданско-правовых отношений. Учебное пособие предназначено для студентов укрупненных групп профессий и специальностей среднего профессионального образования «Экономика и управление», «Средства массовой информации и информационно-библиотечное дело», изучающих дисциплины «Организация рекламной деятельности и связи с общественностью», «Реклама и связи с общественностью», «Правовое обеспечение рекламной деятельности», «Рекламная деятельность», «Организация рекламной деятельности».
Asplenium micropaleatum M. Kessler & A. R. Sm., Candollea
Creators: name: Kessler, Michael
name: Smith, Alan R.
Publisher: name: Zenodo
Year: 2018
Subjects: Biodiversity
Taxonomy
Plantae
Tracheophyta
Polypodiopsida
Polypodiales
Aspleniaceae
Asplenium
Asplenium micropaleatum

description: Asplenium micropaleatum M.Kessler & A.R.Sm., Candollea 61: 308. 2006.
 Range:— Peru and Bolivia (CO, LP).
 Ecology:— Fairly common; terrestrial and saxicolous in humid forests; 1250–3300 m.
 Notes:— Jonas (2011) was unable to find any consistent molecular and morphological traits (besides rhizome scale length) to separate this species from A. achalense. A similar, presumably undescribed species, but with larger leaves and much thicker, flattened rhizomes, is known from Colombia and Ecuador.
description: Published as part of Kessler, Michael & Smith, Alan R., 2018, Prodromus of a fern flora for Bolivia. XXIX. Aspleniaceae, pp. 259-280 in Phytotaxa 344 (3) on pages 259-280, DOI: 10.11646/phytotaxa.344.3.6, http://zenodo.org/record/13720625
title: CI-Re-Implantationen – Indikation, Ergebnisse und Herausforderungen
Creators: name: Volkenstein, Stefan
name: Lehmann, Martin
name: Loke, Laura
name: Dazert, Stefan
Publisher: name: German Medical Science GMS Publishing House
Year: 2019
Subjects: subject: 610 Medical sciences; Medicine

description: Einleitung: Die Anzahl an zukünftig durchzuführenden Re-Implantationen nach erfolgreicher Versorgung von tauben und schwerhörigen Patienten mit Cochlea-Implantaten (CIs) wird deutlich ansteigen. Dies liegt unter anderem an der Ausweitung des Indikationsspektrums, sowie der rasanten technischen[zum vollständigen Text gelangen Sie über die oben angegebene URL]
description: Jahrestagung der Vereinigung Westdeutscher Hals-Nasen-Ohren-Ärzte
Perfect Quantum Cosmological Bounce
Creators: name: Gielen, Steffen
name: Turok, Neil
Publisher: name: arXiv
Year: 2015
Subjects: subject: High Energy Physics - Theory (hep-th)
subject: General Relativity and Quantum Cosmology (gr-qc)
subject: FOS: Physical sciences

description: We study quantum cosmology with conformal matter comprising a perfect radiation fluid and a number of conformally coupled scalar fields. Focusing initially on the collective coordinates (minisuperspace) associated with homogeneous, isotropic backgrounds, we are able to perform the quantum gravity path integral exactly. The evolution describes a "perfect bounce", in which the Universe passes smoothly through the singularity. We extend the analysis to spatially flat, anisotropic universes, treated exactly, and to generic inhomogeneous, anisotropic perturbations treated at linear and nonlinear order. This picture provides a natural, unitary description of quantum mechanical evolution across a cosmological bounce. We provide evidence for a semiclassical description in which all fields pass "around" the cosmological singularity along complex classical paths.
description: 5 pages, revtex, APS style, 1 figure. v4: minor changes in presentation, new title, matches published version
CD19xCD3 DART protein mediates human B-cell depletion in vivo in humanized BLT mice
Creators: name: Silvestri, Guido
name: Tsai, Perry
name: Garcia, J Victor
name: Thayer, William O
name: Nordstrom, Jeffrey L
name: Liu, Liqin
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2016

description: Novel therapeutic strategies are needed for the treatment of hematologic malignancies; and bispecific antibody-derived molecules, such as dual-affinity re-targeting (DART) proteins, are being developed to redirect T cells to kill target cells expressing tumor or viral antigens. Here we present our findings of specific and systemic human B-cell depletion by a CD19xCD3 DART protein in humanized BLT mice. Administration of the CD19xCD3 DART protein resulted in a dramatic sustained depletion of human CD19+ B cells from the peripheral blood, as well as a dramatic systemic reduction of human CD19+ B-cell levels in all tissues (bone marrow, spleen, liver, lung) analyzed. When human CD8+ T cells were depleted from the mice, no significant B-cell depletion was observed in response to CD19xCD3 DART protein treatment, confirming that human CD8+ T cells are the primary effector cells in this in vivo model. These studies validate the use of BLT humanized mice for the in vivo evaluation and preclinical development of bispecific molecules that redirect human T cells to selectively deplete target cells.
title: Glomus tympanicum Tumor: Fallbeispiele mit Gegenüberstellung der operativen Maßnahmen
Creators: name: Dauti, Veton
name: Jadeed, Rawad
name: Ilgner, Justus
name: Alt, Jan Patrick
name: Westhofen, Martin
Publisher: name: German Medical Science GMS Publishing House
Year: 2019
Subjects: subject: 610 Medical sciences; Medicine

description: Einleitung: Der Glomus tympanicum-Tumor ist eine Neoplasie des Mittelohres, die aus dem chromaffinen Paraganglion tympanicum hervorgeht. Es handelt sich um einen gefäßreichen, lokal destruierenden, gutartigen Tumor. Zusammen mit dem Glomus jugulare-Tumor stellen die beiden Neoplasien die häufigsten[zum vollständigen Text gelangen Sie über die oben angegebene URL]
description: Jahrestagung der Vereinigung Westdeutscher Hals-Nasen-Ohren-Ärzte
Asplenium poloense Rosenst., Repert. 2018, Spec. Nov.
Creators: name: Kessler, Michael
name: Smith, Alan R.
Publisher: name: Zenodo
Year: 2018
Subjects: Biodiversity
Taxonomy
Plantae
Tracheophyta
Polypodiopsida
Polypodiales
Aspleniaceae
Asplenium
Asplenium poloense

description: Asplenium poloënse Rosenst., Repert. Spec. Nov. Regni Veg. 12: 469. 1913.
 Range:— Peru and Bolivia (BE, CO, LP, PA); perhaps also along the Andes from Venezuela to Ecuador.
 Ecology:— Fairly common; terrestrial and saxicolous, in humid forests; 250–1200 m.
 Notes:— Distinction from A. pulchellum uncertain, but pinnae of A. poloënse are usually more deeply incised or lobed, halfway to costae or more along the acroscopic side. Differs from A. tabinense by smaller size, paler petioles and rachises, and occurrence at low elevation.
description: Published as part of Kessler, Michael & Smith, Alan R., 2018, Prodromus of a fern flora for Bolivia. XXIX. Aspleniaceae, pp. 259-280 in Phytotaxa 344 (3) on pages 259-280, DOI: 10.11646/phytotaxa.344.3.6, http://zenodo.org/record/13720625
Binary phase oscillation of two mutually coupled semiconductor lasers
Creators: name: Utsunomiya, Shoko
name: Namekata, Naoto
name: Takata, Kenta
name: Akamatsu, Daisuke
name: Inoue, Shuichiro
name: Yamamoto, Yoshihisa
Publisher: name: arXiv
Year: 2015
Subjects: subject: Quantum Physics (quant-ph)
subject: Optics (physics.optics)
subject: FOS: Physical sciences

description: A two-site Ising model is implemented as an injection-locked laser network consisting of a single master laser and two mutually coupled slave lasers. We observed ferromagnetic and antiferromagnetic orders in the in-phase and out-of-phase couplings between the two slave lasers. Their phase difference is locked to either 0 or p even if the coupling path is continuously modulated. The system automatically selects the oscillation frequency to satisfy the in-phase or out-of-phase coupling condition, when the mutual coupling dominates over the injection-locking by the master laser.
description: 12 pages, 9 figures
title: Technologies for Digitalization and Decarbonization of Individual Mobility
Creators: name: Baumgarte, Felix
Publisher: name: University of Bayreuth
Year: 2022
Subjects: subject: Individual mobility
subject: Decarbonization
subject: Electric vehicles
subject: Digital technologies
subject: Energy management
subject: 330

description: The fight against climate change requires rapid global action to decarbonize both industry and society. Among the largest emitters of climate-damaging greenhouse gases, changes in indi-vidual road mobility are necessary to substantially reduce emissions. Several promising measures can be promptly implemented to reduce emissions from individual mobility. Three of the most important are the electrification of individual road mobility with electric vehicles, the integration of electric vehicles as an essential part of the electricity system, and new concepts of mobility to reduce car ownership. However, the decarbonization potential in individual measures can be leveraged only through the efficient use of digital technologies and compre-hensively designed information systems. Consequently, a great need exists for research at the intersection of information systems, including digital technologies and sustainable mobility. The information systems community must help address the global challenge of decarbonizing individual mobility. This work includes seven research papers addressing the decarbonization and digitalization of transportation and energy to enable sustainable individual mobility. Therefore, this thesis first addresses the management of electric vehicle charging to accelerate the expansion of charging options. Second, it addresses the integration of electric vehicles into the electricity system, including in combination with renewable energy sources and the provi-sion of flexibility enabled by digital technologies. Third, it describes the need for innovation in shared mobility solutions, such as carsharing, to reduce car ownership. This thesis positions itself at the intersection of green information systems and information systems for innovative mobility business models. It bridges research into sustainable energy systems and the devel-opment of new business models and services in the mobility sector.
title: Программирование на С++
Creators: name: ELS IPR BOOKS
Publisher: name: Профобразование
Year: 2024

description: В учебном пособии представлено описание основ алгоритмизации и программирования на языке С++. Рассмотрены виды вычислительных процессов, базовые алгоритмы, указатели и массивы, текстовые данные, структуры данных, файлы данных. Учебное пособие предназначено для студентов, обучающихся по укрупненным группам специальностей среднего профессионального образования «Информатика и вычислительная техника, «Информационная безопасность», изучающих дисциплины «Программирование», «Основы алгоритмизации и программирования».
An NMR-Guided Screening Method for Selective Fragment Docking and Synthesis of a Warhead Inhibitor
Creators: name: Khattri, Ram B.
name: Morris, Daniel L.
name: Davis, Caroline M.
name: Bilinovich, Stephanie M.
name: Debord, Michael A.
name: Leeper, Thomas C.
name: Caras, Andrew J.
name: Panzner, Matthew J.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2016

description: Selective hits for the glutaredoxin ortholog of Brucella melitensis are determined using STD NMR and verified by trNOE and (15)N-HSQC titration. The most promising hit, RK207, was docked into the target molecule using a scoring function to compare simulated poses to experimental data. After elucidating possible poses, the hit was further optimized into the lead compound by extension with an electrophilic acrylamide warhead. We believe that focusing on selectivity in this early stage of drug discovery will limit cross-reactivity that might occur with the human ortholog as the lead compound is optimized. Kinetics studies revealed that lead compound 5 modified with an ester group results in higher reactivity than an acrylamide control; however, after modification this compound shows little selectivity for bacterial protein versus the human ortholog. In contrast, hydrolysis of compound 5 to the acid form results in a decrease in the activity of the compound. Together these results suggest that more optimization is warranted for this simple chemical scaffold, and opens the door for discovery of drugs targeted against glutaredoxin proteins-a heretofore untapped reservoir for antibiotic agents.
Asplenium polyphyllum Bertol., Novi Comment. Acad. Sci. Inst. Bononiensis
Creators: name: Kessler, Michael
name: Smith, Alan R.
Publisher: name: Zenodo
Year: 2018
Subjects: Biodiversity
Taxonomy
Plantae
Tracheophyta
Polypodiopsida
Polypodiales
Aspleniaceae
Asplenium
Asplenium polyphyllum

description: Asplenium polyphyllum Bertol., Novi Comment. Acad. Sci. Inst. Bononiensis 4: 443. 1840.
 Range:— Central Mexico to Panama; Venezuela; Ecuador to Bolivia (CO, LP).
 Ecology:— Uncommon; terrestrial in humid timberline forests with Polylepis Ruiz & Pav. and Weinmannia L., often growing among rocks and on ravine banks; 3100–4000 m.
 Notes:— A member of the A. monanthes complex (Dyer et al. 2012, 2013).
description: Published as part of Kessler, Michael & Smith, Alan R., 2018, Prodromus of a fern flora for Bolivia. XXIX. Aspleniaceae, pp. 259-280 in Phytotaxa 344 (3) on pages 259-280, DOI: 10.11646/phytotaxa.344.3.6, http://zenodo.org/record/13720625
title: Tracing protein native radicals under in vitro and in vivo conditions via EPR
title: E. coli class Ia ribonucleotide reductase as a paradigm
titleType: AlternativeTitle
Creators: name: Meichsner, Shari Lorraine
Publisher: name: TU Dortmund
Year: 2022
Subjects: subject: EPR
subject: Elektronenspinresonanz
subject: Protein
subject: In-cell
subject: Tyrosyl radical
subject: Ribonucleotide reductase
subject: Unnatural amino acids
subject: Metalloenzymes
subject: DEER
subject: PELDOR
subject: 540
subject: EPR
subject: Metalloenzym

description: Although more than a hundred thousand protein structures are registered in the Protein Data Bank, there are still more open questions than answers. One of these proteins that have captivated researchers for decades is ribonucleotide reductase, short RNR. Due to its central role in every living organism, RNR has been the focus of research on several occasions, but many questions remain unanswered despite numerous studies. Moreover, information about RNR structure in its natural environment, namely living cells, is entirely lacking. A method that can be used for protein structure elucidation is electron paramagnetic resonance, shortly EPR. EPR is also widely used for RNR studies since the enzyme has a native radical that is used for catalysis. In this work, state-of-the-art EPR spectroscopy was used to address some of the open questions related to RNR. Advanced EPR techniques were used to determine the structure of the stable tyrosyl radical Y122• in E. coli class Ia RNR in living whole E. coli cells at high resolution. Furthermore, EPR was used to investigate the in vivo radical distribution of RNR. In addition to in-cell EPR, three other applications of magnetic resonance spectroscopy are highlighted in this work. First, insights into the development process of a new spin label are given, using RNR as a model system. Through these studies, azidophenylalanine was identified as a suitable candidate that could contribute to the field of in-cell protein structure elucidation via spin labeling. Second, a high-pressure apparatus was used to study the effect of pressure on E. coli RNRs structure by EPR. Finally, independently of RNR, cw-EPR was used to characterize the long-lived C60 radical anion generated in a molecular coordination cage.
Chemical enrichment of the pre-solar cloud by supernova dust grains
Creators: name: Heitsch, Fabian
name: Goodson, Matthew D.
name: Frazer, Christopher C.
name: Luebbers, Ian
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2016

description: The presence of short-lived radioisotopes (SLRs) in Solar system meteorites has been interpreted as evidence that the Solar system was exposed to a supernova shortly before or during its formation. Yet results from hydrodynamical models of SLR injection into the proto-solar cloud or disc suggest that gas-phase mixing may not be efficient enough to reproduce the observed abundances. As an alternative, we explore the injection of SLRs via dust grains as a way to overcome the mixing barrier. We numerically model the interaction of a supernova remnant containing SLR-rich dust grains with a nearby molecular cloud. The dust grains are subject to drag forces and both thermal and non-thermal sputtering. We confirm that the expanding gas shell stalls upon impact with the dense cloud and that gas-phase SLR injection occurs slowly due to hydrodynamical instabilities at the cloud surface. In contrast, dust grains of sufficient size ( ≳ 1 μm) decouple from the gas and penetrate into the cloud within 0.1 Myr. Once inside the cloud, the dust grains are destroyed by sputtering, releasing SLRs and rapidly enriching the dense (potentially star-forming) regions. Our results suggest that SLR transport on dust grains is a viable mechanism to explain SLR enrichment.