title: Ð Ð¾Ð»Ñ ÐºÐ°Ð¿ÑÐ¸Ð´Ð° Ð°Ð´ÐµÐ½Ð¾Ð°ÑÑÐ¾ÑÐ¸Ð¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð²Ð¸ÑÑÑÐ½Ð¾Ð³Ð¾ Ð²ÐµÐºÑÐ¾ÑÐ° Ð² ÑÑÐ°Ð½ÑÐ´ÑÐºÑÐ¸Ð¸ ÐºÐ»ÐµÑÐ¾Ðº ÑÐµÑÑÐ°ÑÐºÐ¸ Ð¼ÑÑÐ¸
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¼Ð°Ð³Ð¸ÑÑÑÐ°
titleType: Subtitle
title: Adeno associated viral vector capsid role in a mouse retinal cells transduction
Creators: name: ÐÑÐ°Ð´ÑÑÐ¾Ð², ÐÐ»ÑÑ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2024
Subjects: Ð°Ð´ÐµÐ½Ð¾Ð°ÑÑÐ¾ÑÐ¸Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐµ Ð²Ð¸ÑÑÑÐ½ÑÐµ Ð²ÐµÐºÑÐ¾ÑÐ°
Ð³ÐµÐ½Ð½Ð°Ñ ÑÐµÑÐ°Ð¿Ð¸Ñ
ÑÐ¾Ð»Ñ ÐºÐ°Ð¿ÑÐ¸Ð´Ð°
Ð¾Ð¿ÑÐ¾Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ðµ Ð¿ÑÐ¾ÑÐµÐ·Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ
Ð´ÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸Ð²Ð½Ð°Ñ ÑÐµÑÑÐ°ÑÐºÐ°
ÑÑÐ°Ð½ÑÐ´ÑÐºÑÐ¸Ñ ÑÐµÑÑÐ°ÑÐºÐ¸
ÑÐ»ÐµÐºÑÑÐ¾ÑÐµÑÐ¸Ð½Ð¾Ð³ÑÐ°ÑÐ¸Ñ
adenoassociated viral vectors
gene therapy
the role of capsid
optogenetic prosthetics
degenerative retina
retinal transduction
electroretinography

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description: This work is devoted to the study of one of the fundamental aspects of optogenetic prosthetics â the role of the viral capsid in the transduction of target retinal cells. The research was focused primarily on the study of artificially created promising capsids and their application to prosthetics of the degenerative retina. In the course of the work, the efficiency of mouse retinal cell transduction was compared using the example of two AAV capsids developed based on different serotypes: capsid 7m8 derived from AAV2 and PHP.eB derived from AAV9. Viral vectors with the target transgene were injected using intravitreal injections, the registration of retinal functionality in dynamics was performed using ERG, after a month the expression of the target transgene was evaluated using histological methods. The results of the ERG measurements were fitted by the Naka-Rushton equation. From the point of view of optogenetic prosthetics, the transduction of retinal cells and the expression of the target transgene were much more effective when using a viral vector packed in a 7m8 capsid. AAV-PHP.eB vector mainly infected photoreceptors and pigment epithelium, centers of infection when using it were extremely rare and had an uneven distribution over the area of the retina.