title: ÐÐ·Ð¼ÐµÐ½ÐµÐ½Ð¸Ðµ ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÑÐ¾ÑÑÐ¾ÑÐ½Ð¸Ñ ÑÐµÑÑÐ°ÑÐºÐ¸ Ñ Ð¼ÑÑÐµÐ¹ Ñ Ð½Ð°ÑÐ»ÐµÐ´ÑÑÐ²ÐµÐ½Ð½Ð¾Ð¹ Ð´ÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸ÐµÐ¹ ÑÐ¾ÑÐ¾ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð²
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Changes of the retinal functional state in mice with hereditary degeneration of photoreceptors
Creators: name: ÐÐ¾ÑÑÑÐµÐ½ÐºÐ¾Ð², ÐÑÑÐµÐ½Ð¸Ð¹
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: Ð·ÑÐµÐ½Ð¸Ðµ
ÑÐµÑÑÐ°ÑÐºÐ°
ÑÐ¾ÑÐ¾ÑÐµÑÐµÐ¿ÑÐ¾ÑÑ
On-Ð±Ð¸Ð¿Ð¾Ð»ÑÑÐ½ÑÐµ ÐºÐ»ÐµÑÐºÐ¸
Ð½Ð°ÑÐ»ÐµÐ´ÑÑÐ²ÐµÐ½Ð½Ð°Ñ Ð´ÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸Ñ ÑÐ¾ÑÐ¾ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð²
ÑÐ»ÐµÐºÑÑÐ¾ÑÐµÑÐ¸Ð½Ð¾Ð³ÑÐ°Ð¼Ð¼Ð°
Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸Ñ ÑÐµÑÑÐ°ÑÐºÐ¸
Ð¼ÑÑÐ¸ rd1
ÑÐ°Ð½Ð½Ð¸Ð¹ Ð¾Ð½ÑÐ¾Ð³ÐµÐ½ÐµÐ·
vision
retina
photoreceptors
On-bipolar cells
hereditary degeneration of photoreceptors
electroretinogram
retina morphology
rd1 mice
early ontogenesis

description: ÐÐ°Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð¸ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸ÐºÐµ Ð²ÑÐµÐ¼ÐµÐ½Ð½Ð¾Ð¹ Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐ¸ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð¸ ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð°Ð»ÑÐ½ÑÑ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹, Ð¿ÑÐ¾Ð¸ÑÑÐ¾Ð´ÑÑÐ¸Ñ Ð² ÑÐ°Ð½Ð½ÐµÐ¼ Ð²Ð¾Ð·ÑÐ°ÑÑÐµ Ð² ÑÐµÑÑÐ°ÑÐºÐµ Ð¼ÑÑÐµÐ¹ Ð»Ð¸Ð½Ð¸Ð¸ Ð¡3Ð/Crl, Ð¾Ð±Ð»Ð°Ð´Ð°ÑÑÐ¸Ñ Ð¼ÑÑÐ°ÑÐ¸ÑÐ¼Ð¸ Ð² Ð³ÐµÐ½Ð°Ñ Pde6b Ð¸ Gpr179. Ð ÐºÐ°ÑÐµÑÑÐ²Ðµ ÐºÐ¾Ð½ÑÑÐ¾Ð»ÑÐ½Ð¾Ð¹ Ð³ÑÑÐ¿Ð¿Ñ Ð±ÑÐ»Ð¸ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ñ Ð¼ÑÑÐ¸ Ð»Ð¸Ð½Ð¸Ð¸ C57Bl/6J. Ð ÑÐ¾Ð´Ðµ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ñ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÑ Ð¿Ð¾ Ð³ÐµÐ½Ð¾ÑÐ¸Ð¿Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ñ Ð¼ÑÑÐµÐ¹ Ð´Ð»Ñ Ð¿Ð¾Ð´ÑÐ²ÐµÑÐ¶Ð´ÐµÐ½Ð¸Ñ Ð½Ð°Ð»Ð¸ÑÐ¸Ñ Ð¸ÑÐºÐ¾Ð¼ÑÑ Ð¼ÑÑÐ°ÑÐ¸Ð¹ Ñ Ð¶Ð¸Ð²Ð¾ÑÐ½ÑÑ Ð»Ð¸Ð½Ð¸Ð¸ Ð¡3Ð/Crl. Ð¢Ð°ÐºÐ¶Ðµ Ð±ÑÐ»Ð¸ Ð·Ð°ÑÐµÐ³Ð¸ÑÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ñ ÑÐ»ÐµÐºÑÑÐ¸ÑÐµÑÐºÐ¸Ðµ Ð¾ÑÐ²ÐµÑÑ ÑÐµÑÑÐ°ÑÐºÐ¸ Ð¸ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½ Ð³Ð¸ÑÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ð¹ Ð°Ð½Ð°Ð»Ð¸Ð· Ð´Ð»Ñ Ð¾ÑÐµÐ½ÐºÐ¸ ÐµÑ ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ Ð¸ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð³Ð¾ ÑÐ¾ÑÑÐ¾ÑÐ½Ð¸Ñ, ÑÐ¾Ð¾ÑÐ²ÐµÑÑÑÐ²ÐµÐ½Ð½Ð¾.
 ÐÐ½Ð°Ð»Ð¸Ð· Ð´Ð°Ð½Ð½ÑÑ Ð¾ Ð²Ð¾Ð·ÑÐ°ÑÑÐ½Ð¾Ð¹ Ð´Ð¸Ð½Ð°Ð¼Ð¸ÐºÐµ Ð¿Ð°ÑÐ°Ð¼ÐµÑÑÐ¾Ð² Ð·ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÑÐ½ÐºÑÐ¸Ð¸ Ñ Ð¼ÑÑÐµÐ¹ Ð»Ð¸Ð½Ð¸Ð¸ C57Bl/6J Ð½Ðµ Ð²ÑÑÐ²Ð¸Ð» Ð·Ð½Ð°ÑÐ¸Ð¼ÑÑ Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸Ð¹ Ð² ÑÐ°Ð½Ð½ÐµÐ¼ Ð²Ð¾Ð·ÑÐ°ÑÑÐµ. Ð¢Ð°ÐºÐ¶Ðµ Ð±ÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾, ÑÑÐ¾ Ñ Ð¼ÑÑÐµÐ¹ Ð»Ð¸Ð½Ð¸Ð¸ Ð¡3Ð/Crl Ð½Ð°Ð±Ð»ÑÐ´Ð°ÐµÑÑÑ ÑÑÑÐµÐ¼Ð¸ÑÐµÐ»ÑÐ½Ð°Ñ Ð´ÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸Ñ ÑÐµÑÑÐ°ÑÐºÐ¸ Ð² Ð¿ÐµÑÐ¸Ð¾Ð´ Ñ 18 Ð¿Ð¾ 25 Ð´ÐµÐ½Ñ Ð¶Ð¸Ð·Ð½Ð¸, ÐºÐ°Ðº Ð½Ð° ÑÑÐ½ÐºÑÐ¸Ð¾Ð½Ð°Ð»ÑÐ½Ð¾Ð¼, ÑÐ°Ðº Ð¸ Ð½Ð° Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¾Ð¼ ÑÑÐ¾Ð²Ð½ÑÑ. 
ÐÐ°Ð½Ð½ÑÐµ Ð¾Ð± Ð¸Ð·Ð¼ÐµÐ½ÐµÐ½Ð¸ÑÑ ÑÐ»ÐµÐºÑÑÐ¾ÑÐ¸Ð·Ð¸Ð¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ Ð¸ Ð¼Ð¾ÑÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ ÑÐ°ÑÐ°ÐºÑÐµÑÐ¸ÑÑÐ¸Ðº, Ð¿ÑÐ¾Ð¸ÑÑÐ¾Ð´ÑÑÐ¸Ñ Ð´Ð¾, Ð²Ð¾ Ð²ÑÐµÐ¼Ñ, Ð¸ Ð¿Ð¾ÑÐ»Ðµ Ð³Ð¸Ð±ÐµÐ»Ð¸ ÑÐ¾ÑÐ¾ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð² Ñ Ð¼ÑÑÐµÐ¹ Ð»Ð¸Ð½Ð¸Ð¸ C3H/Crl Ð¿ÑÐµÐ´ÑÑÐ°Ð²Ð»ÑÑÑ Ð²Ð°Ð¶Ð½Ð¾ÑÑÑ Ð´Ð»Ñ Ð¿Ð¾Ð½Ð¸Ð¼Ð°Ð½Ð¸Ñ Ð¿ÑÐ¾ÑÐµÑÑÐ¾Ð² ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ñ Ð¿Ð°ÑÐ¾Ð»Ð¾Ð³Ð¸Ð¹, Ð¿ÑÐ¸Ð²Ð¾Ð´ÑÑÐ¸Ñ Ðº Ð¿Ð¾ÑÐµÑÐµ Ð·ÑÐ¸ÑÐµÐ»ÑÐ½Ð¾Ð¹ ÑÑÐ½ÐºÑÐ¸Ð¸. ÐÐ¾Ð»ÑÑÐµÐ½Ð½ÑÐµ Ð² ÑÐ¾Ð´Ðµ ÑÐ°Ð±Ð¾ÑÑ Ð´Ð°Ð½Ð½ÑÐµ ÑÐ¾ÑÑÐ°Ð²Ð»ÑÑÑ ÐºÐ°ÑÑÐ¸Ð½Ñ Ð¿Ð°ÑÐ¾Ð»Ð¾Ð³Ð¸Ð¸ ÑÐµÑÑÐ°ÑÐºÐ¸ Ñ Ð¶Ð¸Ð²Ð¾ÑÐ½ÑÑ ÑÑÐ¾Ð¹ Ð»Ð¸Ð½Ð¸Ð¸ Ð¸ Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÑ Ð² Ð´Ð°Ð»ÑÐ½ÐµÐ¹ÑÐµÐ¼ Ð¿ÑÐ¾Ð°Ð½Ð°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°ÑÑ ÑÐ¾Ð»Ñ Ð±Ð¸Ð¿Ð¾Ð»ÑÑÐ½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº ÑÐµÑÑÐ°ÑÐºÐ¸ Ð¸ Ð¸Ñ ÑÐ¸Ð³Ð½Ð°Ð»ÑÐ½Ð¾Ð³Ð¾ ÐºÐ°ÑÐºÐ°Ð´Ð° Ð² Ð¿ÑÐ¾ÑÐµÑÑÐµ Ð½Ð°ÑÐ»ÐµÐ´ÑÑÐ²ÐµÐ½Ð½Ð¾Ð¹ Ð´ÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸Ð¸ ÑÐ¾ÑÐ¾ÑÐµÑÐµÐ¿ÑÐ¾ÑÐ¾Ð².
description: The given work is devoted to studying and characterization of time-dependent dynamics of morphological and functional changes occurring at an early age in the retina of murine strain C3H/Crl with mutations in Pde6b and Gpr179 genes. C57Bl/6J mice were used as a control group. During the work, we set up genotyping experiments to confirm the presence of the mutations in C3H/Crl mice. We also recorded retinal electrical photoresponses and performed histological analysis to evaluate the functional and morphological state of retina, respectively.
There were no significant changes in the visual function of C57Bl/6J mice during the early age. We also observed functional and morphological rapid retinal degeneration in C3H/Crl mice during 18-25 day of life.
Experimental data on the dynamics of morphological and electrophysiological changes occurring before, during, and after C3H/Crl mice photoreceptors death is valuable for understanding mechanisms of pathology, which leads to vision loss. The data obtained during the research describes the whole picture of retinal pathological changes occurring in C3H/Crl murine strain and will be helpful in future studies on the role of the retinal bipolar cells and their signalling cascade in the process of hereditary degeneration of photoreceptors.