cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
title: Vielfalt der Stimmen
Creators: name: Stockinger, Alexander
Year: 2010

description: Im Mittelpunkt dieser Arbeit steht das ästhetische Merkmal der Polyperspektivik. Im weiteren Sinne werden darunter Verfahren der Herstellung, Steigerung, Abbildung oder Verschleierung von Komplexität verstanden. Diese Arbeit setzt im Verfahren des sog. "close reading" an drei Schnittstellen an. Diese sind: E.T.A. Hoffmanns literarisches Werk, E.T.A. Hoffmanns Inszenierung und Stilisierung im sozialen Umfeld sowie die Zusammenführung von Werk und Biografik in Form von Barbiers/Offenbachs Oper Hoffmanns Erzählungen. Zunächst wird eine Klärung und Einbettung des Begriffes „Polyperspektivik“ vorgenommen und anschließendin Abschnitt 1.3. auf die mannigfaltigen Mittel zur Herstellung derselben eingegangen. Es wird am Beispiel der literarischen Werke Hoffmanns ein Analyseraster entworfen, der auch in den folgenden Kapiteln zur Darstellung von Polyperspektivik herangezogen wird. Er umfasst vor allem Phänomene aus dem Bereich der Werkgenese sowie der Erzähl- und Wahrnehmungstheorie. Der Abschnitt zur Inszenierung und Stilisierung Hoffmanns als Person (Kapitel 2.) wird ergänzt um Fragen der Konstituierung von Identität und der Theatralität alltäglicher Situationen. Im Kapitel zu Hoffmanns Erzählungen (Kapitel 3.) wird zusätzlich zu den textimmanenten Kriterien der Polyperspektivik auch das Verhältnis der produktiven Rezeption in die Analyse mit einbezogen.
Regulatory T cells in endogenous mouse lymphoma are antigen-specific nTregs and provide a target for immune checkpoint-inhibiting therapies
Creators: name: Bauer, Vera
name: Ahmetlic, Fatima
name: Riedel, Tanja
name: Trautwein, Nico
name: Sparwasser, Tim
name: Stevanovic, Stefan
name: Röcken, Martin
name: Mocikat, Ralph
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: Purpose/Objectives: Foxp3+ regulatory T cells (Tregs) play an important role in maintaining immune homeostasis. In malignant disease, however, Tregs contribute to the generation of an immunosuppressive microenvironment. To establish therapeutic approaches it is necessary to uncover activating and suppressive mechanisms. Immune checkpoint-blocking (ICB) monoclonal antibodies (mAbs) have been shown to elicit cancer regression and long-lasting tumor control. Particular interest was focused to the mutual influence of Treg cells and therapy by ICB.
Materials/Methods: We used a c-MYC transgenic mouse model of spontaneously arising B-cell lymphoma, which mirrors key features of human Burkitt lymphoma. To assess treatment effects mice were intraperitoneally injected with mAbs against PD-1 and CTLA-4. Mice splenocytes and lymphocytes were analyzed by flow cytometry.
Results: An augmented fraction of CD4+Foxp3+Tregs was detected in spleens and lymph nodes of tumor-bearing c-MYC mice. Tregs were involved in suppressing antitumor response because specific ablation of Tregs significantly delayed tumor development. Compared to wildtype mice, c-MYC Treg cells showed an activated phenotype as evidenced by upregulated CD69 and CD137. The high expression of the costimulatory molecule CD137 indicated TCR-specific activation. As intratumoral Tregs were predominantly Nrp-1+Helios+nTregs, we suggested that Tregs recognized tumor-associated self-antigens. We identified MHC class II-restricted self-epitopes, which were prevalent in lymphoma as compared to normal B cells and could be recognized by Treg cells resulting in enhanced proliferation (Ki-67) in vitro. Interestingly, effector T cells (Teffs) were able to recognize the same epitopes. Antigen contact apparently led to upregulation of IL-10. In vitro suppression assays showed that Tregs from c-MYC and wt mice potently suppress proliferation of Teffs in a dose-dependent manner. When preventing cell contacts, Teff proliferation was increased and almost restored upon additional IL-10 neutralization. Tregs from c-MYC mice treated with anti-CTLA-4/anti-PD-1 mAbs revealed a lower suppressive capacity.
Conclusion: In malignant lymphoma, nTregs directed against self epitopes are involved in cancer immune escape. Taken together, the Treg population is a promising target in future immunotherapies and needs to be further investigated.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)
Pastiroma nigrostriata Emeljanov 2019, comb. nov.
Creators: name: Emeljanov, Alexandr F.
Publisher: name: Zenodo
Year: 2019
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hemiptera
Delphacidae
Pastiroma
Pastiroma nigrostriata

description: Pastiroma nigrostriata (Ding et Zhang, 2000), comb. nov.
Mestus nigrostriatus Ding & Zhang, 2000: 61.
description: Published as part of Emeljanov, Alexandr F., 2019, New synonyms and new combinations for some Delphacidae (Hemiptera, Auchenorrhyncha) of Eastern and Central Palaearctic Region, pp. 396-400 in Zootaxa 4559 (2) on page 398, DOI: 10.11646/zootaxa.4559.2.13, http://zenodo.org/record/2626956
Specific targeting of oncogenic mutations using CRISPR-technology to destroy activated oncogenes and enhance immune response
Creators: name: Oppel, Felix
name: Schürmann, Matthias
name: Sudhoff, Holger
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: Purpose/Objectives: In recent decades, molecular biology tools have enabled researchers to modify the genome of human cells. Today, many methods can be employed to inactivate genes, add them or change their expression. However, survival rates for patients affected by highly malignant cancer types have not improved substantially. If methodology like genome editing, which is routinely used in research laboratories, would be available to clinicians, the patients’ prognosis could be strongly improved.
Materials/Methods: CRISPR-Cas9 is a method to precisely target and manipulate genomic loci and recent studies have attempted to use this method as a treatment for genetic diseases including cancer. Some of these approaches target mutant genomic sequences specifically and try to avoid affecting the respective normal loci. We combined knowledge from recent advances in CRISPR-technology with data from cancer genome sequencing studies to develop a concept for the CRISPR-mediated targeting of recurrent or individual mutations in activated oncogenes.
Results: We present strategies to target mutations in activated oncogenes in a mutation-specific way using CRISPR-technology. The induction of DNA double strand breaks in key locations of oncogenic drivers is likely to abolish or reduce their oncogenic potential. CRISPR-molecules effective against highly recurrent mutations might be usable off-the-shelf for all patients with that mutation. Clinical electroporators could improve the delivery of CRISPR-molecules into tumor cells after local or systemic injection. Moreover, when combined for example with checkpoint inhibitors, CRISPR-targeting might enhance the immune-recognition of tumor cells and/or decrease the expression of ligands reducing cellular immune responses. However, considering obvious genetic risks opposing the objected benefits, this approach has to be evaluated carefully in preclinical studies.
Conclusion: We believe that the mutant-specific targeting of oncogenes activated by mutation can be achieved employing the latest CRISPR-technology and that this approach could be combined with immunotherapy.
Current publication: Oppel F, Schürmann M, Goon P, Albers AE, Sudhoff H. Specific Targeting of Oncogenes Using CRISPR Technology. Cancer Research. 2018 Sep 7. PubMed PMID: 30194069.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)
Effects of EGFR-MEK-ERK pathway on PD-L1 expression in head and neck squamous cell carcinoma
Creators: name: Rong, Chao
name: Feng, Bohai
name: Muller, Marie
name: Holzinger, Dana
name: Flechtenmacher, Christa
name: Thierauf, Julia
name: Affolter, Annette
name: Plinkert, Peter
name: Hess, Jochen
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: Objectives: Programmed death ligand-1 (PD-L1) is frequently expressed in human cancers, including head and neck squamous cell carcinoma (HNSCC). However, the regulation of PD-L1 in HNSCC remains largely elusive. The purpose of this study was to address the molecular mechanism of PD-L1 regulation by EGFR-MEK-ERK signaling and to suggest potential clinical relevance of blocking PD-1/PD-L1 in combination with therapeutic options targeting EGFR signaling in HNSCC.
Methods: Correlations of PD-L1 protein expression and key nodes of EGFR-MEK-ERK signaling in HNSCC were analyzed by The Cancer Proteome Atlas (TCPA, http://tcpaportal.org). The impact of drugs targeting the EGFR-MEK-ERK pathway on tumor cell survival, clonal expansion as well as PD-L1 expression were addressed in cell culture models. PD-L1 expression and ERK1/2 phosphorylation (p-ERK1/2) were assessed on tissue microarrays with specimens from primary oropharyngeal squamous cell carcinoma (OPSCC) (n=123 patients) by immunohistochemistry and were compared with clinic-pathological features.
Results: A highly significant association was found between PD-L1 expression and key nodes of EGFR-MEK-ERK signaling analyzing the TCPA-HNSCC dataset. However, no significant association was found between PD-L1 expression and p-ERK1/2 protein level in the TCPA-HNSCC dataset OPSCC cohort. The impact of cetuximab on PD-L1 expression in HNSCC cell lines after short-term or long-term treatment was heterogeneous but independent of ERK1/2 phosphorylation. Treatment with a MEK1/2 inhibitor confirmed that PD-L1 regulation in HNSCC cell lines is largely independent of MEK-ERK signaling. The majority of HNSCC cell lines with long-term cetuximab treatment regained ERK1/2 phosphorylation as compared to short-term treatment. Up-regulation of PD-L1 was observed in most cetuximab-resistant cell lines after long-term treatment.
Conclusion: Our findings suggest a complex and context-dependent PD-L1 regulation upon inhibition of the EGFR-MEK-MAPK signaling cascade and further experimental and clinical studies will be essential to unravel underlying molecular  principles.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)
CD8+ T cell response to HPV16 E7 predicts survival outcome in oropharyngeal cancer
Creators: name: Sudhoff, Holger
name: Masterson, Liam
name: Jani, Piyush
name: Sterling, Jane Carolyn
name: Goon, Peter
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: Immunological response to human papillomavirus (HPV) in the development and progression of HPV16+ oropharyngeal squamous cell carcinoma (OPSCC) (accounting for the majority of viral associated cases) is largely unknown and may provide important insights for new therapeutic strategies. In this prospective clinical trial, we examined cell-mediated immune responses to HPV16 E2, E6 and E7 in peripheral blood using IFN-? enzyme-linked immunosorbent spot assay. CD56(+), CD4(+), CD8(+) and regulatory T cell frequencies were also discerned by flow cytometry. Fifty-one study participants with oropharyngeal carcinoma were recruited. Control subjects were those undergoing tonsillectomy for benign disease. All patients were treated with curative intent by radiotherapy ± chemotherapy. Disease-specific survival was investigated by multivariate analysis. HPV16 DNA was detected in 41/51 of the OPSCC participants. T cell responses against HPV16 E6 or E7 peptides were detected in 33/51 evaluable patients, respectively and correlated with HPV status. Matched pre- and post-treatment T cell responses were available for 39/51 OPSCC cases. Within the whole cohort, elevated post-treatment CD8(+) response to HPV16 E7 correlated with longer disease free survival (multivariate DFS p < 0.03). Within the HPV + OPSCC cohort, a significant increase in regulatory T cells (p < 0.02) was noted after treatment. This is the first
study to provide survival data in OPSCC stratified by cell-mediated immune response to HPV16 peptides. Within the HPV16+ OPSCC cohort, enhanced immunoreactivity to antigen E7 was linked to improved survival. An increase in regulatory T cell frequencies after treatment may suggest that immunosuppression can contribute to a reduced HPV-specific cell-mediated response.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)
Current status of radiotherapy/radiochemotherapy and immunotherapy combinations– a German survey
Creators: name: Käsmann, Lukas
name: Eze, Chukwuka
name: Roengvoraphoj, Olarn
name: Belka, Claus
name: Manapov, Farkhad
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: Purpose/Objetives: Combinations of immunotherapy with radiation or radiochemotherapy are currently under investigation in several cancer types and clinical settings. In this survey, we solicited members of radiation oncology institutions in Germany to review the current status of research in this field, underline critical issues such as oncological benefit, treatment toxicity and obstacles in clinical research.
Method: We surveyed radiation oncology institutions in Germany via an anonymous online questionnaire sent by e-mail to members of the German Radation Oncology Society and yDEGRO (working group of DEGRO e.V.). The questions were based on current studies at their institutions, clinical settings (cancer type, cerebral metastasized situation), emphasizing the oncologic benefit and obstacles of clinical research.
Results: We received a total of 26 responses (61.90% response rate). Of which 23 were completed and returned, hence eligible for further evaluation. The responders represent 14 different departments of radiation oncology at German university hospitals. 14 (60.90%) responders perform radio-immunotherapy studies at their institutions and participate in multicenter studies. Radio-immunotherapy combinations were investigated mainly in head & neck tumors (95%), lung cancer (57%), malignant melanoma (48%) and tumors of the upper GI tract (8.7%). Combinations of radiochemotherapy with immunotherapy were only tested in head & neck cancers (52%), non-small cell lung cancer (NSCLC) (8.70%) and malignant melanoma (4%).The oncologic benefit of radio/radiochemotherapy with immunotherapy is assumed to be effective or very effective by >85% of all responders. Regarding possible obstacles to clinical research of radio/radiochemo-immunotherapy combinations, 21 (91.3%) responders mentioned lack of financial resources, 18 (78.3%) responders shortage of human resources, 10 (43.5%) responders lack of sufficient evidence and 6 (26.1%) lack of ethics committee approval.
Conclusion: Radioimmunotherapy combinations are under investigation at >75% of all participating centers. Head & neck tumors, NSCLC and malignant melanoma are the most frequently tested cancer types. The treatment of intracranial metastatic disease by the combination of radiotherapy and immunotherapy is assumed to be very effective by the majority of responders. Several obstacles for clinical research were collated. The results of
ongoing prospective trials are eagerly awaited and our survey shows great acceptance of new combinations of radio/radiochemo-immunotherapy.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)
Activation of immune response in refractory patients to standard treatment (T.R.A.N.S.L.A.T.E.)
Creators: name: Abbona, Andrea
name: Sangiolo, Dario
name: Merlano, Marco
name: Garrone, Ornella
name: Anna, Merlotti
name: Monteverde, Martino
name: Lo Nigro, Cristiana
name: Falletta, Antonella
name: Occelli, Marcella
name: Varamo, Chiara
name: Aglietta, Massimo
name: Gammaitoni, Loretta
name: Reali, Alessia
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: Purpose/Objectives: The TRANSLATE project started in 2016 to test the immune effects of metronomic cyclophosphamide, daily low-dose IL-2 every other week, and a single flash of radiotherapy (RT) in peripheral blood. The rationale is based on self vaccination induced by radiotherapy (RT) 8 Gy single fraction on one metastatic lesion, T cells expansion by IL-2 treatment and selective Tregs down regulation by lowdose cyclophosphamide administration.
Materials/Methods: We enrolled patients with end-stage breast, colon, kidney and prostate cancer. Analysis was performed at baseline, the day after RT, after 28 days from treatment start and at disease progression. Assay focused on Tregs, CD8+, NK, MDSC, CD3-PD1, IL-2, IL-4, IL-5, IL- 6, IL-10, IL-12, IL-13, IL-17a, TNFα, IFNγ, TGFβ. We divided patients into two groups depending on the time of disease progression (A > 3 months; B, < 3 months). We report preliminary data with the aim to show the changes observed post-RT in 20 pts.
Results: At baseline, group B had higher rates of CD3-PD1, higher IL-2, IL-13, TNFα, and TGFβ; group A had higher IFN γ, IL-4, and IL-12. After RT, we observed a difference between the two groups in the rates of CD3-PD1 (lower in group B) and Tregs (higher in group A). Among cytokines, only TNFα reached a statistical significance (higher in group B). We also observed that TGFβ and IL-6 were higher in group B and IFNγ was higher in group A. The longitudinal analyses showed that CD3-PD1 remained stable between basal and post-RT in group A but decreased in group B. Tregs marginally increased in group A. TNFα, TGFβ, IL-4, IL-6, and IL-12 increased in group B. IL-4 decreased in group A and IL-6 and IL-12 also marginally decreased in the same group. IFNγ slightly increases in group A.
Discussion: The limited number of patients reduced the interpretation of the study. However, following RT a positive trend of Th2 cytokines is observed in patients with early progressing disease, without the expected surge of IFNγ that was instead observed in patients with better outcome. Additional analyses are in progress and will be presented.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)
Additional file 1 of The antioxidant and antimicrobial activity of ethanolic extract in roots, stems, and leaves of three commercial Cymbopogon species
Creators: name: Wahyuni, Dwi Kusuma
name: Kharisma, Viol Dhea
name: Murtadlo, Ahmad Affan Ali
name: Rahmawati, Cici Tya
name: Syukriya, Alvi Jauharotus
name: Prasongsuk, Sehanat
name: Subramaniam, Sreeramanan
name: Wibowo, Anjar Tri
name: Purnobasuki, Hery
Publisher: name: figshare
Year: 2024
Subjects: subject: Biochemistry
subject: Biotechnology
subject: Environmental Sciences not elsewhere classified
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: FOS: Biological sciences
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Plant Biology
subject: Virology

description: Additional file 1. Table of the yield of three commercial Cymbopogon spp. ethanolic extracts.
Which adjuvants for tumor-antigen vaccination induce powerful non-sialylated IgG antibodies?
Creators: name: Bartsch, Yannic
name: Ehlers, Marc
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: Tumor-antigen vaccination is a promissing approach to induce intensive T and B cell and IgG antibody responses for eliminating the tumor. The effector functions of IgG antibodies depend on their subclass and Fc glycosylation pattern. Non-fucosylation and non-sialylation of tumor-antigen-specific IgG antibodies enhance their affinity to activating Fcgamma receptors on immune cells and enhance tumor elimination. Accordingly, it has been suggested that tumor-antigen vaccination should induce non-fucosylated and non-sialylated tumor-antigen-specific IgG antibodies. We have analyzed, which adjuvants induce or not non-sialylated IgG antibodies and what are the responsible mechanisms and have identified an IL-17 dependent mechanism for the generation of non-sialylated IgG antibodies. We conclude that adjuvants interesting for tumor-antigen vaccination can be selected by their potential to induce non-sialylated IgG antibodies.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)
Falcotoya minuscula
Creators: name: Emeljanov, Alexandr F.
Publisher: name: Zenodo
Year: 2019
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hemiptera
Delphacidae
Falcotoya
Falcotoya minuscula

description: Falcotoya minuscula (Horváth, 1897)
Delphax minuscula Horváth, 1897: 622.
= Calligypona minutissima Dubovsky, 1966: 68, 71, syn. nov.
Note. Slight differences in a degree of twist of the penis shaft does not qualify as enough evidence for treating Falcotoya minutissima Dubovsky as a separate species.
description: Published as part of Emeljanov, Alexandr F., 2019, New synonyms and new combinations for some Delphacidae (Hemiptera, Auchenorrhyncha) of Eastern and Central Palaearctic Region, pp. 396-400 in Zootaxa 4559 (2) on page 398, DOI: 10.11646/zootaxa.4559.2.13, http://zenodo.org/record/2626956
Killing melanoma cells with ROS provides immuno-protection against tumor growth in vivo
Creators: name: Freund, Eric
name: Clemen, Ramona
name: Gandhirajan, Rajesh Kumar
name: Bekeschus, Sander
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: Purpose/Objectives: Immunogenic cancer cell death (ICD) delivers inflammatory stimuli to elicit anti-tumor immune responses. Release of adjuvant find-me (HMGB1, ATP) and eat-me (calreticulin; CRT) signals enables DC maturation and cross presentation of tumor antigen to T cells. Effective ICD-inducing therapies are drugs such as anthracyclines, ionizing irradiation, or photodynamic therapies. Intriguingly, these antitumor strategies show concomitant generation of reactive oxygen species (ROS) that can drive cell death and immunogenicity signaling responses through redox relays. In cancer cells, the importance of ROS are underappreciated in the onset of ICD.
Materials/methods: A novel technology, cold physical plasma, was used to generate various ROS that can be effectively delivered to B16F10 melanoma cells. The oxidation and ATP levels as well as expression levels of MHC class I and CRT were measured. Plasma-treated tumor cells were injected into syngeneic mice with a subsequent re-challenge with live cells. Mitomycin C (MMC) and mitoxantrone (MTX) served as negative and positive control, respectively. Lymph node cells of sacrificed mice were co-cultured with melanoma cells, and T cell activation was assessed.
Results: Cold physical plasma oxidized melanoma cells and led to an increase of ATP release as well as MHC class I and CRT expression. Mice “vaccinated” with plasma-treated melanoma cells showed less tumor growth compared to mice receiving MMC-treated cells but more tumor growth compared to positive control MTX. Activation of lymph node-derived T cells ex vivo with melanoma cells was best in MTX mice, followed by the cells from the plasma group, and was lowest in cells from the MMC group.
Conclusion: Not only the event of cell death but also its auxiliary signals are important in the formation of anti-tumor immune responses. ROS play a significant role in this process. Deep phenotyping of ROS-treated melanoma cells will decipher the molecular machinery important in eliciting the responses observed.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)
Designs for spatial and spatio-temporal models
Creators: name: Dasgupta, Subhadra
Publisher: name: Monash University
Year: 2022
Subjects: classificationCode: 401302
subject: Geospatial information systems and geospatial data modelling
classificationCode: 490501
subject: Applied statistics

description: This thesis determines optimal experimental designs for geostatistical models, under various frequentist and Bayesian setups. For simple and ordinary kriging and cokriging, equispaced designs minimize some functions of the prediction error and are proved to be G-optimal and I-optimal. G-optimal designs for universal kriging models are found to be symmetric and concentrated near the boundaries. To address a more practical scenario, deterministic algorithms are proposed for finding retrospective optimal designs by the addition or deletion of points from an already existing design. The theory is illustrated by designing an optimal water monitoring network for an Indian river.
Pre- and intratherapeutic cellular monitoring for dose adjustment of chemotherapy in aggressive lymphoma
Creators: name: Schröder, Julia
name: Rödiger, Stefan
name: Ruhe, Madeleine
name: Deckert, P. Markus
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: Purpose: In eukaryotic cells treated with chemotherapeutic drugs DNA double strand breaks (DSB) are the most severe form of DNA damage which usually leads to apoptotic cell death. Cytotoxic chemotherapy is still a mainstay of cancer therapy, but there are few established methods to individually predict efficacy and tolerability. In most regimens dosage is based on the body surface area, a dimension of little biological precision, and individual dose adaptions are usually performed by clinical guessing or in response to adverse events. This project sets out to examine biological parameters of cell damage.
Methods: In particular, we measure DNA double strand breaks by an automated yH2AX assay applying the Aklides NUK® system. In preliminary work, PBMCs were stimulated with bendamustine or with rituximab, cyclophosphamide, doxorubicin, vincristine, and prednisone, the components of the R-CHOP protocol, to induce DSB.
Results: There was no inter-individual correlation between drug dose and number of foci, whereas does titrations on the cells of each individual donor demonstrated a clear correlation between dose and yH2AX assay result.
Conclusion: The lack of correlation across individuals between yH2AX foci and drug dosage based on standard calculation by body surface area suggests that standardized dosing of chemotherapeutic drugs based on gross physical determinants such as weight or body surface area does not correspond to the individual’s biological response and clinical effect. The future plan of this project is the replacement of standard dosage regimens by biologically based, real-time-adapted individual dosage in lymphoma to reduce toxicity while increasing therapeutic efficacy.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)
Additional file 2 of The antioxidant and antimicrobial activity of ethanolic extract in roots, stems, and leaves of three commercial Cymbopogon species
Creators: name: Wahyuni, Dwi Kusuma
name: Kharisma, Viol Dhea
name: Murtadlo, Ahmad Affan Ali
name: Rahmawati, Cici Tya
name: Syukriya, Alvi Jauharotus
name: Prasongsuk, Sehanat
name: Subramaniam, Sreeramanan
name: Wibowo, Anjar Tri
name: Purnobasuki, Hery
Publisher: name: figshare
Year: 2024
Subjects: subject: Biochemistry
subject: Biotechnology
subject: Environmental Sciences not elsewhere classified
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: FOS: Biological sciences
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Plant Biology
subject: Virology

description: Additional file 2. Table of retention time and percentage area of metabolite profile of three commercial Cymbopogon spp. from Indonesia.
title: The "Italianness" of "The Godfather"
Creators: name: Wallner, Barbara
Year: 2010

description: Im Zentrum der GODFATHER Trilogie steht die Geschichte und Entwicklung einer italo-amerikanischen Mafia-Familie. Dementsprechend viel Platz nimmt die Darstellung italienischer Ethnizität und Kultur ein. Auch in der vorliegenden Arbeit stehen diese beiden Elemente der Filme im Mittelpunkt, wobei nur die ersten beiden Teile zur Analyse herangezogen werden, da sie unmittelbar aufeinander folgen und somit eine Handlungseinheit bilden. Zu Beginn der Arbeit werden verschiedene Einflüsse auf die Filme und ihren Inhalt erläutert. Zum einen werden die GODFATHER Filme im Genre des Gangsterfilms verankert, zum anderen wird die stereotype Darstellung von Italienern und Italo-Amerikanern im US-amerikanischen Kino skizziert. Ein wesentlicher Teil widmet sich dem Konzept Ethnizität und seiner Umsetzung im GODFATHER. Anhand von zwei Ethnizitätsmodellen wird die ethnische Konzeption der einzelnen Figuren beleuchtet. Schließlich werden auch Sprachverwendung und Schauplätze in Bezug auf die Darstellung italienischer Kultur einer näheren Betrachtung unterzogen.
Additional file 1 of Social participation in the promoting activity, independence and stability in early dementia (PrAISED), a home-based therapy intervention for people living with dementia: a realist evaluation
Creators: name: Lorito, Claudio Di
name: Pollock, Kristian
name: Booth, Vicky
name: Howe, Louise
name: Goldberg, Sarah
name: Godfrey, Maureen
name: Dunlop, Marianne
name: Harwood, Rowan H.
name: Wardt, Veronika van der
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Neuroscience
subject: Science Policy
subject: Sociology
subject: FOS: Sociology
subject: Mental Health

description: Supplementary Material 1
Epinotia salicicolana Kuznetzov 1968
Creators: name: Sohn, Jae-Cheon
name: Choi, Sei-Woong
Publisher: name: Zenodo
Year: 2020
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Lepidoptera
Tortricidae
Epinotia
Epinotia salicicolana

description: Epinotia salicicolana Kuznetzov, 1968
 ÜȐjnjḋȁḍ나ǧ (Figs. 1B, 2B)
 Epinotia salicicolana Kuznetzov, 1968: 577. Type locality: Russia, Kuril Islands, Kunashir, near Sernovodsk.
 Description (Fig. 1B). Head. Vertex and frons fuscous. Labial palpus fuscous; 3 rd segment tinged with pale grayish brown apically. Antenna 1/2 as long as forewing, fuscous. Thorax. Forewing length 8.1 mm, fuscous, mottled with brown; costal strigulae grayish brown; dorsal patch subtriangular, white, with dark brown strigulae posteriorly; cilia fuscous. Hindwing and cilia fuscous. Male genitalia (Fig. 2B). Uncus long, narrow, lanceolate apically. Tegumen subtriangular, densely setose on lateral area of distal half. Valva elongate, slightly constricted at basal 1/4, with semicircular cavity basally; costa curved, with bulge subbasally; sacculus subtriangular, densely setose on distal half; cucullus long, narrowly-round apically, broadened basally, circularly-convex ventrobasally, densely setose. Phallus broadened to distal end; cornutal zone with long, hair-like needles, nearly as long as phallus.
 Material examined. 1♂, Jeonnam Prov., Muan-gun, Bokgil-ri (N34°53′ E126°24′, Alt. 25 m), 30 VIII 2006 (SW Choi), [GSN] SJC-1083, NIBR.
 Distribution. Korea, Japan, China (Shaanxi), Taiwan, Russia (Far East), North America.
 Host plants. Salicaceae. Populus tremula var. sieboldii (Miq.) Kudo; Salix spp, including S. caprea L., S. hultenii Flod., S. sachalinensis F. Schmidt (Kuznetsov, 2001; Nasu & Komai, 2013).
 Remarks. This species is similar to Epinotia solandriana (Linnaeus) but differs from the latter in having the smaller dorsal patch on the forewing. The color of dorsal patch on the forewing in Epinotia salicicolana can vary to white or yellowish brown.
description: Published as part of Sohn, Jae-Cheon & Choi, Sei-Woong, 2020, Two species of Olethreutinae (Lepidoptera: Tortricidae) new to Korea, pp. 167-169 in Journal of Species Research 9 (2) on page 169, DOI: 10.12651/JSR.2020.9.2.167, http://zenodo.org/record/13141202
Additional file 2 of Social participation in the promoting activity, independence and stability in early dementia (PrAISED), a home-based therapy intervention for people living with dementia: a realist evaluation
Creators: name: Lorito, Claudio Di
name: Pollock, Kristian
name: Booth, Vicky
name: Howe, Louise
name: Goldberg, Sarah
name: Godfrey, Maureen
name: Dunlop, Marianne
name: Harwood, Rowan H.
name: Wardt, Veronika van der
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Neuroscience
subject: Science Policy
subject: Sociology
subject: FOS: Sociology
subject: Mental Health

description: Supplementary Material 2
Brief an Johann I Bernoulli
Creators: name: Montmort, Pierre Rémond de
Publisher: name: [s.n.]
Year: 1704
Subjects: Descartes, René1596-1650
DinostratusMathematicusv4. Jh
Fontenelle, Bernard Le Bovier de1657-1757
L'Hôpital, Charlotte de1671-1737
L'Hôpital, Guillaume François Antoine de1661-1704
Fantet de Lagny, Thomas1660-1734
Leibniz, Gottfried Wilhelm1646-1716
Malebranche, Nicolas1638-1715
Newton, Isaac1643-1727
Nicole, François1683-1758
Reyneau, Charles René1656-1728
Rolle, Michel1652-1719
Varignon, Pierre1654-1722

description: von Pierre Rémond de Montmort
description: Öffentliche Bibliothek der Universität Basel
description: Französisch
description: Digitalisierung=Digitization=Numérisation TIFF 05.04.2015
description: Beigelegt ein einseitig beschriebenes Blatt (8 x 13,5 cm)
Flastena fumipennis
Creators: name: Emeljanov, Alexandr F.
Publisher: name: Zenodo
Year: 2019
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hemiptera
Delphacidae
Flastena
Flastena fumipennis

description: Flastena fumipennis (Fieber, 1866)
Delphax fumipennis Fieber, 1866: 527.
= Calligypona uzbekistanica Dubovsky, 1966: 72, syn. nov.
Note. The male genitalia structure of Calligypona uzbekistanica illustrated by Dubovsky (1966, 14:34-36) in the original description of the species differs from this of Flastena fumipennis (Fieber) published by Nast (1975) and Holzinger et al. (2003) only in minute details which could be treated as an interspecific variability.
description: Published as part of Emeljanov, Alexandr F., 2019, New synonyms and new combinations for some Delphacidae (Hemiptera, Auchenorrhyncha) of Eastern and Central Palaearctic Region, pp. 396-400 in Zootaxa 4559 (2) on page 398, DOI: 10.11646/zootaxa.4559.2.13, http://zenodo.org/record/2626956
Metal oxide/sulfur composite cathodes for room temperature lithium-sulfur and sodium-sulfur batteries
Creators: name: Ghosh, Arnab
Publisher: name: Monash University
Year: 2021
Subjects: classificationCode: 340604
subject: Electrochemistry

description: Alkali metal-sulfur batteries (such as Li-S and Na-S batteries) theoretically can store 3-5 times higher energy-density in comparison to commercial lithium-ion batteries. However, alkali metal-sulfur batteries are plagued with a fundamental problem of gradual dissolution of polysulfide intermediates into the liquid electrolyte from the cathode, which causes inferior cycling stability. My dissertation describes development of various metal oxide/sulfur composite cathodes to overcome the polysulfide dissolution issue. Metal oxides could immobilize the polysulfide intermediates through strong chemical interaction and thus control their gradual dissolution into. Several characterization techniques are employed to understand the chemical interaction between different metal oxides and the intermediate polysulfides.
Additional file 3 of Social participation in the promoting activity, independence and stability in early dementia (PrAISED), a home-based therapy intervention for people living with dementia: a realist evaluation
Creators: name: Lorito, Claudio Di
name: Pollock, Kristian
name: Booth, Vicky
name: Howe, Louise
name: Goldberg, Sarah
name: Godfrey, Maureen
name: Dunlop, Marianne
name: Harwood, Rowan H.
name: Wardt, Veronika van der
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Neuroscience
subject: Science Policy
subject: Sociology
subject: FOS: Sociology
subject: Mental Health

description: Supplementary Material 3
Paradelphacodes gvosdevi
Creators: name: Emeljanov, Alexandr F.
Publisher: name: Zenodo
Year: 2019
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hemiptera
Delphacidae
Paradelphacodes
Paradelphacodes gvosdevi

description: Paradelphacodes gvosdevi (Mitjaev, 1980)
Chloriona gvosdevi Mitjaev, 1980: 36.
= Chloriona adunca Ding, 1981: 56, syn. nov.
= Paradelphacodes insolitus Dmitriev, 2000: 281, syn. fide Dmitriev & McKamey, 2013: 111.
Paradelphacodes gvosdevi: Mitjaev, 2002: 104.
= Paradelphacodes gvosdevi Mitjaev, 1980, = P. insolitus Dmitriev, 2000: Dmitriev & McKamey, 2013.
Note. A peculiar species similar to the members of the genus Chloriona Fieber according to some characters (light green or light yellow general coloration, long widely places styles, dorsal margin of pygofer bridge (phragma) with a process) originally was described in this genus (Mitjaev, 1980). Later Dmitriev (2000) described this species as Paradelphacodes insolitus, however, recently he corrected himself and placed P. insolitus in synonymy to Ch. gvosdevi with the arguments confirmed generic position of Ch. gvosdevi as Paradelphacodes (Dmitriev & McKamey, 2013).
description: Published as part of Emeljanov, Alexandr F., 2019, New synonyms and new combinations for some Delphacidae (Hemiptera, Auchenorrhyncha) of Eastern and Central Palaearctic Region, pp. 396-400 in Zootaxa 4559 (2) on page 398, DOI: 10.11646/zootaxa.4559.2.13, http://zenodo.org/record/2626956
Extracellular Vesicles as biomarkers for cancer progression and cancer associated VTE
Creators: name: Gieseler, Frank
name: Ender, Fanny
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: Microvesicles (MVs) represent a subgroup of extracellular vesicles (EVs) emerging from various cells by blebbing of their outer membrane. Therefore, they share features such as membrane composition and antigenicity with their parental cells. Released by many immune and tumor cells, MVs act as intercellular messengers, account for horizontal gene transfer and can activate the coagulation system. With the aim to investigate their relevance for tumor cell biology, we characterized MVs released by human tumor cell lines of various origins in the absence or presence of TNF-a. After stimulation, we used the combination of low and high-speed centrifugation to enrich MVs from cell culture supernatants. We analyzed the presentation of phosphatidylserine (PS) and tissue factor (TF) activity on the cell surface and investigated their potency to induce tumor cell migration. In all tumor cell lines, TNF-a stimulation enhanced the release of MVs. While the expression of PS was universally increased, an elevated activity of procoagulant TF could be detected on MVs from lung, pancreatic, and colon carcinoma, but not from breast and ovarian cancer cell lines. Functionally, TNF-a stimulation significantly increased the potency of MVs to induce tumor cell migration. In conclusion, inflammatory conditions promote the release of MVs with increased procoagulant activity from tumor cell lines in vitro. PS-containing and TF-expressing MVs may account for systemic activation of the coagulation system as seen in cancer patients and, since they induce tumor cell migration, they may serve as biomarkers for tumor progression. Publication: Cell Biology International 2018.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)
Pseudodelphacodes flaviceps
Creators: name: Emeljanov, Alexandr F.
Publisher: name: Zenodo
Year: 2019
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Insecta
Hemiptera
Delphacidae
Pseudodelphacodes
Pseudodelphacodes flaviceps

description: Pseudodelphacodes flaviceps (Fieber, 1866)
Delphax flaviceps Fieber, 1866: 527.
= Calligypona tricolorata Dlabola, 1961: 274 –275, syn. nov.
= Delphacodes fenestratus Emeljanov, 1972: 212 –213, syn, nov.
= Delphacodes (Proscopus) fenestratus: Emeljanov, 1982: 74.
Note. Holzinger et al. (2003) placed Proscopus Emeljanov, 1982 in synonymy with Pseudodelphacodes Wagner, 1963, but retained P. tricoloratus and P. fenestratus as valid names. According to my study of the materials from Mongolia, Middle Asia, and Germany, deposited in the Zoological Institute of the Russian Academy of Sciences (Saint Petersburg, Russia), both names belong to a single species with slight geographical and individual variability.
description: Published as part of Emeljanov, Alexandr F., 2019, New synonyms and new combinations for some Delphacidae (Hemiptera, Auchenorrhyncha) of Eastern and Central Palaearctic Region, pp. 396-400 in Zootaxa 4559 (2) on page 399, DOI: 10.11646/zootaxa.4559.2.13, http://zenodo.org/record/2626956
Electrolyte development based on ionic liquids for Eco-friendly sodium ion battery
Creators: name: Chinnareddy Vekateswarlu Manohar
Publisher: name: Monash University
Year: 2019
Subjects: subject: 30604 Electrochemistry
subject: FOS: Chemical sciences

description: Sodium ion batteries are known as potentially cost effective for grid storage applications. However, organic solvents are commonly used as main electrolytes and this make the batteries unsafe. This thesis aims to produce a high energy density, safe sodium ion battery based on ionic liquid electrolytes. Solid electrolyte inter phase characterization studies are performed on the cathode and anode to understand the electrolyte side reactions in the batteries. A cost effective 3.3V sodium ion battery was demonstrated using a sodium vanadium phosphate cathode material, a bio-derived hard carbon anode material with an ionic liquid electrolyte.
Immune modulation by extracellular vesicles released from mesenchymal cells
Creators: name: Seifert, Martina
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: Mesenchymal cells were described for their potency to interact with human immune cells and to modulate thereby immune responses. Besides mesenchymal stromal cells (MSC) from diverse tissue sources, like bone marrow and umbilical cord, also mesenchymal adherent cells within the heart tissue were able to attenuate and modulate induced immune cell activation or inflammatory processes. Mechanistic studies with MSC support the hypothesis, that mainly paracrine acting molecules, in particular extracellular vesicles (EVs), are responsible for the observed functional effects. EVs are known as potent intercellular communicators by delivering proteins, lipids, RNA and other small signaling molecules to a recipient cell. They can be discriminated by their size and biogenesis into the subsets of apoptotic bodies (diameter > 1μm), microvesicles (diameter range = 1 - 0.1 μm) and exosomes (diameter < 0.1 μm). While microvesicles and apoptotic bodies are shedded from the plasma membrane, exosomes originate from intracellular located multivesicular bodies, which have to fuse with the plasma membrane for their release into the extracellular space. Crosstalk of EVs from MSCs with immune cells is a secured fact, but the way of up-take into the target cells and especially the mechanism of immune modulation are not entirely understood. In our study, we isolated and characterized EVs from a human mesenchymal cardiac cell type regarding their potential to modulate induced immune responses in vitro. The presence of typical EV surface markers like tetraspanins (CD9, CD63, CD81) was confirmed as well as their low expression of HLA-molecules, which indicates a general low immunogenicity. Furthermore, EVs were able to attenuate triggered T cell proliferation accompanied by significantly reduced levels of pro-inflammatory cytokines (IFNγ, TNFα), which was highly dependent on the presence of CD14-positive cells. Interestingly, the EVs of cardiac mesenchymal cells induced a changed phenotype on these myeloid cells; most prominently a reduced HLA-DR and CD86 expression, but enhanced levels for CD206 and PD-L1.


Future studies have to identify key molecular pathways involved in EVs` crosstalk with immune cells and to estimate the benefits but also the risks of this new therapeutic based on mesenchymal cells.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)
Role of glycolysis for intestinal inflammation and carcinogenesis
Creators: name: Hamm, Jacob
name: Klischies, Kenneth
name: Sommer, Nina
name: Rosenstiel, Philip
name: Sommer, Felix
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: Purpose/Objectives: Hexokinase (HK) catalyzes the first step of glycolysis and thereby determines the total glycolysis rate. HK and glycolysis are important for inflammation and carcinogenesis. Immune and cancer cells have elevated HK levels and an increased glycolysis rate to fuel their energetic demand. Inhibition of HK in cancer cells is protective. We showed previously that the microbiome modulates HK expression [1] and activity in intestinal epithelial cells (IECs). Now, we aim to test whether ablation of HK in the intestine protects from inflammation and colon cancer and whether modulation of HK through the microbiota could be a novel therapeutic option.


Materials/Methods: To that end, we generated HK-IEC mice lacking HK specifically in IECs and subjected them to experimental models of intestinal inflammation (dextran sulfate sodium, DSS) or colitis-associated carcinogenesis (azoxymethane-DSS, AOM-DSS). We also analysed the microbiome composition using 16S amplicon sequencing and transcriptional responses using RNA sequencing. We investigated the regulatory function of specific bacteria on HK using gnotobiotic mouse models and in vitro stimulation experiments. Finally, we investigated HK expression in patients with intestinal inflammation and colon cancer.


Results: HK-IEC mice showed reduced susceptibility to intestinal inflammation. Susceptibility to colitis-associated carcinogenesis is currently being evaluated. We identified specific bacteria and metabolites, which modulate HK expression. Finally, we found that HK is dysregulated during intestinal inflammation and in tumours of colon cancer patients.


Conclusion: Inhibition of epithelial glycolysis protects from acute intestinal inflammation and potentially colitis-associated carcinogenesis. Probiotic administration of HK-regulating bacteria or their modulation via prebiotics might serve as a novel non-invasive approach for inflammation and cancer therapy.


Reference


[1] Sommer, F., I. Nookaew, N. Sommer, P. Fogelstrand, and F. Backhed, Site-specific programming of the host epithelial transcriptome by the gut microbiota. Genome Biol, 2015. 16(1): p. 62. Funded by Deutsche Forschungsgemeinschaft CRC1182 “Evolution and Function of Metaorganisms” and Excellence Cluster 306 “Inflammation at Interfaces”.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)
Additional file 4 of Social participation in the promoting activity, independence and stability in early dementia (PrAISED), a home-based therapy intervention for people living with dementia: a realist evaluation
Creators: name: Lorito, Claudio Di
name: Pollock, Kristian
name: Booth, Vicky
name: Howe, Louise
name: Goldberg, Sarah
name: Godfrey, Maureen
name: Dunlop, Marianne
name: Harwood, Rowan H.
name: Wardt, Veronika van der
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Neuroscience
subject: Science Policy
subject: Sociology
subject: FOS: Sociology
subject: Mental Health

description: Supplementary Material 4
MALDI-imaging: Identification of protein signatures of genomic instability in colorectal cancers
Creators: name: Gemoll, Timo
name: Klein, Oliver
name: Miroll, Elena
name: Thorns, Christoph
name: Habermann, Jens
Publisher: name: Proceedings on Cancer-Immuno-Oncology
Year: 2018

description: Background: Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) has improved over the years and is increasingly being used for biomarker discovery directly from human tissue. Since DNA aneuploidy has been identified as a prognostic factor for epithelial malignancies, we compared diploid and aneuploid colon cancer tissues of the colorectum by IMS.


Material and Methods: DNA image cytometry determined the ploidy status of tissue samples that were subsequently subjected to MALDI-IMS. After obtaining protein and peptide profiles through direct analysis of both, fresh frozen and FFPE tissue sections, a discovery and a validation set were used to predict ploidy and disease status by applying proteomic classification algorithms. Identification of m/z values were performed by using nUPLC mass spectrometry from an adjacent tissue section. Clinical target validations were performed by immunohistochemistry using in-house compiled tissue microarrays (TMA).


Results: Classification algorithms categorized normal mucosa and colon carcinoma as well as diploid and aneuploid colon cancers correctly. Proteins could be identified to be differentially expressed between diploid and aneuploid samples that showed expression differences also in clinical samples using a tissue microarray of normal mucosa, diploid and aneuploid colorectal carcinomas.


Conclusion: Our data underscore the potential of MALDI-IMS proteomic algorithms for individualized medicine to reveal significant molecular details from distinct tumor subtypes such as different ploidy types.
description: Proceedings on Cancer-Immuno-Oncology, Vol 1 No 1 (2018)