title: ÐÐ½ÑÐ¸Ð¼Ð¸ÐºÑÐ¾Ð±Ð½ÑÐµ ÑÐ¸ÐºÐ»Ð¸ÑÐµÑÐºÐ¸Ðµ Ð»Ð¸Ð¿Ð¾Ð¿ÐµÐ¿ÑÐ¸Ð´Ñ ÐºÐ°Ðº Ð¸Ð½Ð³Ð¸Ð±Ð¸ÑÐ¾ÑÑ ÑÐ»Ð¸ÑÐ½Ð¸Ñ Ð¼ÐµÐ¼Ð±ÑÐ°Ð½
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Molecular mechanisms of inhibition of membrane fusion by natural extracts
Creators: name: ÐÐ»Ð¾Ð´ÐµÐµÐ²Ð°, ÐÐ¾Ð»Ð¸Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2022
Subjects: ÑÐ»Ð¸ÑÐ½Ð¸Ðµ Ð¼ÐµÐ¼Ð±ÑÐ°Ð½
Ð¿ÑÐ¸ÑÐ¾Ð´Ð½ÑÐµ ÑÐºÑÑÑÐ°ÐºÑÑ
ÑÐºÑÑÑÐ°ÐºÑ ÑÐ°Ð³Ð¸
ÑÐºÑÑÑÐ°ÐºÑ Ð»Ð¸-ÑÑÑÐµÐ² Ð¾Ð±Ð»ÐµÐ¿Ð¸Ñ Ð¸
ÑÐºÑÑÑÐ°ÐºÑ ÑÐµÐ¼ÑÐ½ Ð³ÑÐµÐ¹Ð¿ÑÑÑÑÐ°
membrane fusion
natural extracts
chaga extract
sea buckthorn leaf ex-tract
grapefruit seed extract

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description: The subject of the graduate qualification work is "Molecular mechanisms of inhibition of membrane fusion by natural extracts". The aim of the study was to test extracts of the leaves of the fir, grapefruit seeds and chaga for the presence of an inhibitory effect during the fusion of model lipid membranes, followed by the identification of molecular mechanisms of antifusogenic activity of the tested compounds. During the work, fluorimetric measurements of calcein leakage from liposomes were performed to study changes in the permeability of lipid membranes under influence of the tested natural extracts. The ability of extracts of sea buckthorn leaves, chaga, grapefruit seeds and their components (flavonols and tetracyclic triterpenoids) to inhibit the fusion of lipid vesicles induced by various inducers was evaluated. Morphological changes in the structure of the leaves under the action of natural extracts in the absence and presence of polyethylene glycol were visualized, using confocal fluorescent microscopy. It was noted that with calcium-induced fusion, inhibition by sea buckthorn leaf extract is 90%, and by chaga extract is 97%. With polyethylene glycol-mediated fusion, the inhibitory activity is shown only by the extract of chaga in the amount of 79%. The obtained results indicate the presence of antifusogenic properties in extracts of sea buckthorn leaves and chaga. The study of natural extracts contributes to the development of antiviral drugs, the action of which is based on a change in the physicochemical properties of the lipid membrane of the virus.