cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Polycomb Repressive Complex 2 Is Dispensable for Maintenance of Embryonic Stem Cell Pluripotency
Creators: name: Yee, Della
name: Chamberlain, Stormy J.
name: Magnuson, Terry
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2008

description: Polycomb Repressive Complex 2 (PRC2) methylates histone H3 tails at lysine 27 and is essential for embryonic development. The three core components of PRC2, Eed, Ezh2, and Suz12, are also highly expressed in embryonic stem (ES) cells where they are postulated to repress developmental regulators and thereby prevent differentiation to maintain the pluripotent state. We performed gene expression and chimera analyses on low and high passage Eednull ES cells to determine whether PRC2 is required for the maintenance of pluripotency. We report here that, although developmental regulators are overexpressed in Eednull ES cells, both low and high passage cells are functionally pluripotent. We hypothesize that they are pluripotent because they maintain expression of critical pluripotency factors. Given that EED is required for stability of EZH2, the catalytic subunit of the complex, these data suggest that PRC2 is not necessary for the maintenance of the pluripotent state in ES cells. We propose a positive-only model of embryonic stem cell maintenance, where positive regulation of pluripotency factors is sufficient to mediate stem cell pluripotency.
FFT Interpolation from Nonuniform Samples Lying in a Regular Grid
Creators: name: Selva, J.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Information Theory (cs.IT)
subject: FOS: Computer and information sciences

description: This paper presents a method to interpolate a periodic band-limited signal from its samples lying at nonuniform positions in a regular grid, which is based on the FFT and has the same complexity order as this last algorithm. This kind of interpolation is usually termed "the missing samples problem" in the literature, and there exists a wide variety of iterative and direct methods for its solution. The one presented in this paper is a direct method that exploits the properties of the so-called erasure polynomial, and it provides a significant improvement on the most efficient method in the literature, which seems to be the burst error recovery (BER) technique of Marvasti's et al. The numerical stability and complexity of the method are evaluated numerically and compared with the pseudo-inverse and BER solutions.
description: Submitted to the IEEE Transactions on Signal Processing
Using historical spy satellite photographs and recent remote sensing data to identify high-conservation-value forests
Creators: name: Munteanu, Catalina
name: Senf, Cornelius
name: Nita, Mihai Daniel
name: Sabatini, Francesco Maria
name: Oeser, Julian
name: Seidl, Rupert
name: Kuemmerle, Tobias
Publisher: name: Humboldt-Universität zu Berlin
Year: 2021
Subjects: subject: anthropogenic pressure
subject: conservation value
subject: distribution modeling
subject: forest continuity
subject: forest structure
subject: spy satellite
subject: imagery
subject: temperate forests
subject: 550 Geowissenschaften

description: High-conservation-value forests (HCVFs) are critically important for biodiversity and ecosystem service provisioning, but they face many threats. Where systematic HCVF inventories are missing, such as in parts of Eastern Europe, these forests remain largely unacknowledged and therefore often unprotected. We devised a novel, transferable approach for detecting HCVFs based on integrating historical spy satellite images, contemporary remote sensing data (Landsat), and information on current potential anthropogenic pressures (e.g., road infrastructure, population density, demand for fire wood, terrain). We applied the method to the Romanian Carpathians, for which we mapped forest continuity (1955–2019), canopy structural complexity, and anthropogenic pressures. We identified 738,000 ha of HCVF. More than half of this area was identified as susceptible to current anthropogenic pressures and lacked formal protection. By providing a framework for broad-scale HCVF monitoring, our approach facilitates integration of HCVF into forest conservation and management. This is urgently needed to achieve the goals of the European Union's Biodiversity Strategy to maintain valuable forest ecosystems.
Battista Botticelli, “maestro di legname” di Vasari
Creators: name: Cecchi, Alessandro
Publisher: name: Opus Incertum
Year: 2018

description: The life and works of Battista di Bartolomeo Botticelli have remained to this day almost completely unknown. This article, thanks to new documentary research and a re-examination of Vasari's sources, aims to shed light upon the career of a craftsman who executed the monumental carved platform ‘alla veneziana’ of the Salone dei Cinquecento, and was also Vasari's trusted carpenter. He was already active in Rome in the 1550's under the rule of Pope Julius III, where he constructed stages and friezes in the palaces belonging to the Pope and to Cardinal Ricci on Via Giulia. He was also active in Palazzo Vecchio, where he designed carved ceilings for the Quartiere di Eleonora and the platform of the Terrace of Saturn in the Quartiere degli Elementi, works which underline the central role played by Botticelli in Vasari's fertile worksite, in a field such as the art of carpentry which still requires today new and in-depth research by scholars.
description: Opus Incertum, Vol 3 (2017)
SFRP2 Regulates Cardiomyogenic Differentiation by Inhibiting a Positive Transcriptional Autofeedback Loop of Wnt3a
Creators: name: Deb, Arjun
name: Davis, Bryce H.
name: Guo, Jian
name: Dzau, Victor J.
name: Zhang, Zhiping
name: Mu, Hui
name: Huang, Jing
name: Ni, Aiguo
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2008

description: Wnts comprise a family of 20 lipid-modified glycoproteins in mammals and play critical roles during embryological development and organogenesis of several organ systems, including the heart. They are required for mesoderm formation and have been implicated in promoting cardiomyogenic differentiation of mammalian embryonic stem cells, but the underlying mechanisms regulating Wnt signaling during cardiomyogenesis remain poorly understood. In this report, we show that in a pluripotent mouse embryonal carcinoma stem cell line, SFRP2 inhibits cardiomyogenic differentiation by regulating Wnt3a transcription. SFRP2 inhibited early stages of cardiomyogenesis, preventing mesoderm specification and maintaining the cells in the undifferentiated state. Using a gain- and loss-of-function approach, we demonstrate that although addition of recombinant SFRP2 decreased Wnt3a transcription and cardiomyogenic differentiation, silencing of Sfrp2 led to enhanced Wnt3a transcription, mesoderm formation, and increased cardiomyogenesis. We show that the inhibitory effects of SFRP2 on Wnt transcription are secondary to interruption of a positive feedback effect of Wnt3a on its own transcription. Wnt3a increased its own transcription via the canonical pathway and TCF4 family of transcription factors, and the inhibitory effects of SFRP2 on Wnt3a transcription were associated with disruption of downstream canonical Wnt signaling. The inhibitory effects of Sfrp2 on Wnt3a expression identify Sfrp2 as a “checkpoint gene,” which exerts its control on cardiomyogenesis through regulation of Wnt3a transcription.
El “palcho della sala” del palacio Ramírez de Montalvo en Florencia (1567-1570)
Creators: name: Plaza, Carlos
Publisher: name: Opus Incertum
Year: 2018

description: The main hall of the piano nobile is a key axis in Ammannati’s architectural project for the Ramírez de Montalvo palace in Florence and its status is entrusted to its wooden celiling, as well as to its monumental hearth and its spatial organisation. This architectural setting is also key to the palace project of the Spanish court architect for his protector Duke Cosimo I’s strategy to flaunt the social position of his most loyal collaborators in the new order of the Principato. Through the further exploration of the design and construction of the ‘palcho della sala’ of the Palace of Ramírez de Montalvo this study focuses on inherent features of the architecture of the Florentine secondo Cinquecento: from the complex relationship between architecture and politics based on the role played by Cosimo I and other court clients, to the more technological matters concerning the organisation of the work-site or – regarding specifically the issue of the soffitto ligneo – the work of Bartolomeo Ammannati and Giorgio Vasari and their figurative and technical models for this project in relation to the architecture of the time.
description: Opus Incertum, Vol 3 (2017)
Stability of the $β$-equilibrated dense matter and core-crust transition in neutron stars
Creators: name: Atta, Debasis
name: Basu, D. N.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Nuclear Theory (nucl-th)
subject: FOS: Physical sciences

description: The stability of the $β$-equilibrated dense nuclear matter is analyzed with respect to the thermodynamic stability conditions. Based on the density dependent M3Y effective nucleon-nucleon interaction, the effects of the nuclear incompressibility on the proton fraction in neutron stars and the location of the inner edge of their crusts and core-crust transition density and pressure are investigated. The high-density behavior of symmetric and asymmetric nuclear matter satisfies the constraints from the observed flow data of heavy-ion collisions. The neutron star properties studied using $β$-equilibrated neutron star matter obtained from this effective interaction for a pure hadronic model agree with the recent observations of the massive compact stars. The density, pressure and proton fraction at the inner edge separating the liquid core from the solid crust of neutron stars are determined to be $ρ_t=$ 0.0938 fm$^{-3}$, P$_t=$ 0.5006 MeV fm$^{-3}$ and x$_{p(t)}=$ 0.0308, respectively.
description: 7 pages including 2 figures and 2 tables. arXiv admin note: substantial text overlap with arXiv:1404.4360, arXiv:0905.1599, arXiv:0707.4620; and text overlap with arXiv:0807.4477 by other authors
Identification of GW bursts in high noise using Padé filtering
Creators: name: Perotti, L.
name: Regimbau, T.
name: Vrinceanu, D.
name: Bessis, D.
Publisher: name: arXiv
Year: 2014
Subjects: subject: General Relativity and Quantum Cosmology (gr-qc)
subject: FOS: Physical sciences

description: We consider the case of highly noisy data coming from two different antennas, each data set containing a damped signal with the same frequency and decay factor but different amplitude, phase, starting point and noise. Formally, we treat the first data set as real numbers and the second one as purely imaginary and we add them together. This complex set of data is analyzed using Padé Approximations applied to its Z-transform. Complex conjugate poles are representative of the signal; other poles represent the noise and this property allows to identify the signal even in strong noise. The product of the residues of the complex conjugate poles is related to the relative phase of the signal in the two channels and is purely imaginary when the signal amplitudes are equal. Examples are presented on the detection of a fabricated gravitational wave burst received by two antennas in the presence of either white or highly colored noise.
description: 13 pages, 14 figures
Soffitti figurati nelle chiese di Roma
Creators: name: Conforti, Claudia
Publisher: name: Opus Incertum
Year: 2018

description: The late-renaissance and baroque churches of Rome frequently show coffered wooden ceilings with relief figures of saints. The essay focuses on those plastic figurations, linking them with both the outcomes of the Council of Trent concerning the catechistic use of images and the new religiosity propagated by the Counter-Reformation. Among those ceilings the essay investigates the one at San Lorenzo in Damaso (1587), dismantled in the nineteenth century, which depicts in bas-relief the glory of the Martyr and some episodes of his life. Before the ceiling of San Lorenzo, this type of figuration was limited to symbolic, iconic and laconic elements, eloquent but not dramatic, as in the magnificent ceilings of San Giovanni in Laterano and, in part, of the Ara Coeli, both executed by the famous French lignarius Flaminio Boulanger.
description: Opus Incertum, Vol 3 (2017)
Regenerative Effects of Transplanted Mesenchymal Stem Cells in Fracture Healing
Creators: name: Landis, Benjamin
name: Granero-Moltó, Froilán
name: Myers, Timothy J.
name: Miga, Michael I.
name: Mortlock, Douglas P.
name: O'Rear, Lynda
name: Spagnoli, Anna
name: Weis, Jared A.
name: Jansen, E. Duco
name: Longobardi, Lara
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2009

description: Mesenchymal stem cells (MSC) have a therapeutic potential in patients with fractures to reduce the time of healing and treat non-unions. The use of MSC to treat fractures is attractive as it would be implementing a reparative process that should be in place but occurs to be defective or protracted and MSC effects would be needed only for the repairing time that is relatively brief. However, an integrated approach to define the multiple regenerative contributions of MSC to the fracture repair process is necessary before clinical trials are initiated. In this study, using a stabilized tibia fracture mouse model, we determined the dynamic migration of transplanted MSC to the fracture site, their contributions to the repair process initiation and their role in modulating the injury-related inflammatory responses. Using MSC expressing luciferase, we determined by bioluminescence imaging that the MSC migration at the fracture site is time- and dose-dependent and, it is exclusively CXCR4-dependent. MSC improved the fracture healing affecting the callus biomechanical properties and such improvement correlated with an increase in cartilage and bone content, and changes in callus morphology as determined by micro-computed-tomography and histological studies. Transplanting CMV-Cre-R26R-LacZ-MSC, we found that MSC engrafted within the callus endosteal niche. Using MSC from BMP-2-Lac-Z mice genetically modified using a bacterial artificial chromosome system to be β-gal reporters for BMP-2 expression, we found that MSC contributed to the callus initiation by expressing BMP-2. The knowledge of the multiple MSC regenerative abilities in fracture healing will allow to design novel MSC-based therapies to treat fractures.
Mechanically Induced Focal Adhesion Assembly Amplifies Anti-Adipogenic Pathways in Mesenchymal Stem Cells
Creators: name: Oguz, Ipek
name: Rubin, Janet
name: Xie, Zhihui
name: Rubin, Clinton
name: Guilluy, Christophe
name: Sen, Buer
name: Burridge, Keith
name: Case, Natasha
name: Styner, Maya
name: Thomas, Jacob
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: The fate of pluripotent mesenchymal stem cells (MSC) is determined through integration of chemical, spatial, and physical signals. The suppression of MSC adipogenesis by mechanical stimuli, which requires Akt-induced inhibition of glycogen synthase kinase 3β (GSK3β) with β-catenin activation, can be enhanced by repetitive dosing within a single day. Here, we demonstrate that reapplication of cyclic strain within a 24-hour period leads to amplification of both Akt activation and its subsequent inhibition of GSK3β, such that total cycle number can be reduced while still inhibiting adipogenesis. Amplification of Akt signaling is facilitated by a dynamic restructuring of the cell in response to mechanical signals, as evidenced by a transient increase in focal adhesion (FA) number and increased RhoA activity. Preventing FA assembly or development of tension blocks activation of Akt by mechanical signals, but not by insulin. This indicates that the FA infrastructure is essential to the physical, but not necessarily the chemical, sensitivity, and responsiveness of the cell. Exploiting the transient nature of cytoskeletal remodeling may represent a process to enhance cell responsiveness to mechanical input and ultimately define the fate of MSCs with a minimal input.
Nondecoupling of charged scalars in Higgs decay to two photons and symmetries of the scalar potential
Creators: name: Bhattacharyya, Gautam
name: Das, Dipankar
Publisher: name: arXiv
Year: 2014
Subjects: subject: High Energy Physics - Phenomenology (hep-ph)
subject: FOS: Physical sciences

description: A large class of two- and three-Higgs-doublet models with discrete symmetries has been employed in the literature to address various aspects of flavor physics. We analyse how the precision measurement of the Higgs to diphoton signal strength would severely constrain these scenarios due to the nondecoupling behavior of the charged scalars, to the extent that the number of additional scalar doublets can be constrained no matter how heavy the nonstandard scalars are. We demonstrate that if the scalar potential is endowed with appropriate global continuous symmetries together with soft breaking parameters, decoupling can be achieved thanks to the unitarity constraints on the mass-square differences of the heavy scalars.
description: 9 pages, 2 figures, several clarifying remarks and references added, to appear in Phys Rev D
Mesenchymal Stem Cells Expressing Insulin-like Growth Factor-I (MSCIGF) Promote Fracture Healing and Restore New Bone Formation in Irs1 Knockout Mice: Analyses of MSCIGF Autocrine and Paracrine Regenerative Effects
Creators: name: Yan, Yun
name: Myers, Timothy J.
name: Longobardi, Lara
name: Spagnoli, Anna
name: Rubin, Janet
name: Li, Tieshi
name: Weis, Jared A.
name: Case, Natasha
name: Granero-Moltó, Froilán
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2011

description: Failures of fracture repair (non-unions) occur in 10% of all fractures. The use of mesenchymal stem cells (MSC) in tissue regeneration appears to be rationale, safe and feasible. The contributions of MSC to the reparative process can occur through autocrine as well as paracrine effects. The primary objective of this study is to find a novel mean, by transplanting primary cultures of bone marrow-derived MSC expressing insulin-like growth factor-I (MSCIGF), to promote these seed-and-soil actions of MSC to fully implement their regenerative abilities in fracture repair and non-unions. MSCIGF or traceable MSCIGF-Lac-Z were transplanted into wild-type or insulin-receptor-substrate knock-out (Irs1−/−) mice with a stabilized tibia fracture. Healing was assed using biomechanical testing, micro-computed-tomography (µCT) and histological analyses. We found that systemically transplanted MSCIGF through autocrine and paracrine actions improved the fracture mechanical strength and increased new bone content while accelerating mineralization. We determined that IGF-I adapted the response of transplanted MSCIGF to promote their differentiation into osteoblasts. In vitro and in vivo studies showed that IGF-I-induced induced osteoglastogenesis in MSC was dependent of an intact IRS1-PI3K signaling. Furthermore, using Irs1−/− mice as a non-union fracture model through altered IGF signaling, we demonstrated that the autocrine effect of IGF-I on MSC restored the fracture new bone formation and promoted the occurrence of a well-organized callus that bridged the gap; a callus that basically absent in Irs1−/− left untransplanted or transplanted with MSC. We provided evidence of effects and mechanisms for transplanted MSCIGF in fracture repair and potentially to treat non-unions.
Il supercoelium di San Marco a Roma tra disegno e costruzione
Creators: name: Bova, Sara
Publisher: name: Opus Incertum
Year: 2018

description: Constructed between May 1467 and February 1468 by the carpenters Giovannino and Marco di Pietro de’ Dolci and the illuminator Giuliano degli Amadei, the wooden coffered ceiling at San Marco al Campidoglio constitutes one of the early works carried out under Pope Paul II's for the renewal of the basilica, to which he had been appointed as a cardinal, as part of the wider fabbrica of the complex of palazzo Venezia. This artwork represents the first case of the use of this all’antica solution for the ceiling of a church hall, following the tradition begun by Brunelleschi at San Lorenzo in Florence. However, regarding the details of the mouldings and the remarkable variety of shapes used for the flowers placed at the centre of each coffer, the ceiling of San Marco shows a closer continuity with examples in stone from the Roman Imperial period, such as the ones decorating the intradoses of the arch of Titus and of the ptéron of the temple of Mars Ultor.
description: Opus Incertum, Vol 3 (2017)
Airway Epithelial Progenitors Are Region Specific and Show Differential Responses to Bleomycin-Induced Lung Injury
Creators: name: Randell, Scott H.
name: Liang, Jiurong
name: Jiang, Dianhua
name: Chen, Huaiyong
name: Lee, Joo-Hyeon
name: Brockway, Brian L.
name: Rackley, Craig R.
name: Matsumoto, Keitaro
name: Stripp, Barry R.
name: Kim, Carla F.
name: Noble, Paul W.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2012

description: Mechanisms that regulate regional epithelial cell diversity and pathologic remodeling in airways are poorly understood. We hypothesized that regional differences in cell composition and injury-related tissue remodeling result from the type and composition of local progenitors. We used surface markers and the spatial expression pattern of an SFTPC-GFP transgene to subset epithelial progenitors by airway region. Green fluorescent protein (GFP) expression ranged from undetectable to high in a proximal-to-distal gradient. GFPhi cells were subdivided by CD24 staining into alveolar (CD24neg) and conducting airway (CD24low) populations. This allowed for the segregation of three types of progenitors displaying distinct clonal behavior in vitro. GFPneg and GFPlow progenitors both yielded lumen containing colonies but displayed transcriptomes reflective of pseudostratified and distal conducting airways, respectively. CD24lowGFPhi progenitors were present in an overlapping distribution with GFPlow progenitors in distal airways, yet expressed lower levels of Sox2 and expanded in culture to yield undifferentiated self-renewing progeny. Colony-forming ability was reduced for each progenitor cell type after in vivo bleomycin exposure, but only CD24lowGFPhi progenitors showed robust expansion during tissue remodeling. These data reveal intrinsic differences in the properties of regional progenitors and suggest that their unique responses to tissue damage drive local tissue remodeling. Disclosure of potential conflicts of interest is found at the end of this article.
Il soffitto delle Sibille nel palazzo del cardinale Della Valle a Roma
Creators: name: Grieco, Lorenzo
Publisher: name: Opus Incertum
Year: 2018

description: The survey and the analysis of the luxury setup in a building can be decisive in reconstructing the function and the spatial succession of its interior rooms. Wooden ceilings, which are representative objects as well as technological devices, share this vocation. The paper considers the ceilings of the Roman palace of cardinal Andrea della Valle (1463-1534), which clarify the distributional changes affecting the building through time. Particularly, the opulence and the orientation of the coffers in the first landing suggest the use of this space as a vestibule. The entire layout of the piano nobile should be considered according to a different distribution. Therefore, the current antechamber, known as the room of the Sibyls because of the painting on the ceiling, was used as a private chapel, as suggested by the theme of the decorations. The stylistic analysis of the Sibyls and the comparison with some artworks from the 16th century made possible their attribution to Francesco Salviati (1510-1563). Furthermore, the existence of three tables on the same subject led to new considerations regarding their history.
description: Opus Incertum, Vol 3 (2017)
Radiative and dilepton decays of the hadronic molecule Zc(3900)+
Creators: name: Gutsche, Thomas
name: Kesenheimer, Matthias
name: Lyubovitskij, Valery E.
Publisher: name: arXiv
Year: 2014
Subjects: subject: High Energy Physics - Phenomenology (hep-ph)
subject: FOS: Physical sciences

description: The newly observed hidden-charm meson Zc(3900) with quantum numbers J(P) = 1(+) is considered as a hadronic molecule composed of DD*. We give detailed predictions for the decay modes Zc(+) -> J/psi + pi(+) + gamma and Zc(+) -> J/psi + pi(+) + l(+) + l(-) (l = e, mu) in a phenomenological Lagrangian approach.
description: 13 pages, 19 figures
I soffitti lignei di palazzo Stati Cenci Maccarani a Roma
Creators: name: Antonucci, Micaela
Publisher: name: Opus Incertum
Year: 2018

description: The palace of Cristoforo Paolo Stati (1498-1550), built between the Pantheon and piazza Navona in the rione Sant’Eustachio, is one of the few architectures of Giulio Pippi, aslo known as Romano (1499?-1546) in Rome. The construction of the palace probably began before Giulio moved to Mantova in 1524, and after the marriage of Cristoforo Stati and Faustina Cenci in 1520. Despite its small size, the building has a noble and solemn appearance and rich interior decorations, comparable to those of prestigious palaces of Cardinals and Popes; in its “public” rooms the wooden ceilings and the frescoes attributable to Perino del Vaga’s studio, celebrate the nobility, richness and artistic culture of its owner. The essay focuses on the analysis of the 16th century core of the palace and in particular on the wooden coffered ceilings, whose original features can be reconstructed thanks to the newly explored records of the 20th century restorations in the archives of the Technical Service of the Senate of the Italian Republic.
description: Opus Incertum, Vol 3 (2017)
Breastmilk Is a Novel Source of Stem Cells with Multilineage Differentiation Potential: Breastmilk Is a Novel Source of Stem Cells
Creators: name: Beltran, Adriana
name: Metzger, Philipp
name: Blancafort, Pilar
name: Stuebe, Alison M.
name: Filgueira, Luis
name: Chetwynd, Ellen
name: Lai, Ching Tat
name: Twigger, Alecia-Jane
name: Hassiotou, Foteini
name: Hartmann, Peter E.
name: Trengove, Naomi
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2012

description: The mammary gland undergoes significant remodeling during pregnancy and lactation, which is fuelled by controlled mammary stem cell (MaSC) proliferation. The scarcity of human lactating breast tissue specimens and the low numbers and quiescent state of MaSCs in the resting breast have hindered understanding of both normal MaSC dynamics and the molecular determinants that drive their aberrant self-renewal in breast cancer. Here, we demonstrate that human breastmilk contains stem cells (hBSCs) with multilineage properties. Breastmilk cells from different donors displayed variable expression of pluripotency genes normally found in human embryonic stem cells (hESCs). These genes included the transcription factors (TFs) OCT4, SOX2, NANOG, known to constitute the core self-renewal circuitry of hESCs. When cultured in the presence of mouse embryonic feeder fibroblasts, a population of hBSCs exhibited an encapsulated ESC-like colony morphology and phenotype and could be passaged in secondary and tertiary clonogenic cultures. While self-renewal TFs were found silenced in the normal resting epithelium, they were dramatically upregulated in breastmilk cells cultured in 3D spheroid conditions. Furthermore, hBSCs differentiated in vitro into cell lineages from all three germ layers. These findings provide evidence that breastmilk represents a novel and noninvasive source of patient-specific stem cells with multilineage potential and establish a method for expansion of these cells in culture. They also highlight the potential of these cells to be used as novel models to understand adult stem cell plasticity and breast cancer, with potential use in bioengineering and tissue regeneration.
I soffitti di palazzo Farnese a Roma: tradizione e innovazione
Creators: name: D’Amelio, Maria Grazia
Publisher: name: Opus Incertum
Year: 2018

description: Between 1545 and 1625, twenty-five wooden ceilings were executed in Palazzo Farnese, in Rome, not to mention the ones lost due to collapses or in the fires of 1612, 1615 and 1701. The inventory is much broader than in any other private palace of Rome, and helps to understand the change in taste that, reflected in the form of this constructive element, suggests the transformation of Roman residential architecture in the sixteenth century. Palazzo Farnese is a living laboratory where old-fashioned ceilings with 'unlimited' extension, still linked to a system based on wooden beams, and those of original creation become essential models for the architecture of Modern Rome and of Europe at large.
description: Opus Incertum, Vol 3 (2017)
Influence of the mass distribution on the magnetic field topology
Creators: name: Raynaud, Raphaël
name: Petitdemange, Ludovic
name: Dormy, Emmanuel
Publisher: name: arXiv
Year: 2014
Subjects: subject: Solar and Stellar Astrophysics (astro-ph.SR)
subject: Fluid Dynamics (physics.flu-dyn)
subject: FOS: Physical sciences

description: Context. Three-dimensional spherical dynamo simulations carried out within the framework of the anelastic approximation have revealed that the established distinction between dipolar and multipolar dynamos tends to be less clear than it was in Boussinesq studies. This result was first interpreted as a direct consequence of the existence of a larger number of models with a high equatorial dipole contribution, together with an intermediate dipole field strength. However, this finding has not been clearly related to specific changes that would have been introduced by the use of the anelastic approximation. Aims. In this paper, we primarily focus on the effects of choosing of a different mass distribution. Indeed, it is likely to have as large consequences as would taking a stratified reference state into account, especially when comparing our results to previous Boussinesq studies. Methods. Our investigation is based on a systematic parameter study of weakly stratified anelastic dynamo models. Results. We show that the tendencies highlighted in previous anelastic dynamo simulations are already present in the Boussinesq limit. Thus they cannot be systematically related to anelastic effects. Actually, a central mass distribution can result in changes in the magnetic field topology that are mainly due to the concentration of convective cells close to the inner sphere.
The Minimum S-Divergence Estimator under Continuous Models: The Basu-Lindsay Approach
Creators: name: Ghosh, Abhik
name: Basu, Ayanendranath
Publisher: name: arXiv
Year: 2014
Subjects: subject: Statistics Theory (math.ST)
subject: Applications (stat.AP)
subject: Methodology (stat.ME)
subject: FOS: Mathematics
subject: FOS: Computer and information sciences

description: Robust inference based on the minimization of statistical divergences has proved to be a useful alternative to the classical maximum likelihood based techniques. Recently Ghosh et al. (2013) proposed a general class of divergence measures for robust statistical inference, named the S-Divergence Family. Ghosh (2014) discussed its asymptotic properties for the discrete model of densities. In the present paper, we develop the asymptotic properties of the proposed minimum S-Divergence estimators under continuous models. Here we use the Basu-Lindsay approach (1994) of smoothing the model densities that, unlike previous approaches, avoids much of the complications of the kernel bandwidth selection. Illustrations are presented to support the performance of the resulting estimators both in terms of efficiency and robustness through extensive simulation studies and real data examples.
description: Pre-Print, 34 pages
Alcuni soffitti lignei in Vaticano tra Pio IV e Gregorio XIII
Creators: name: Balzarotti, Valentina
Publisher: name: Opus Incertum
Year: 2018

description: This paper analyses some polychrome coffered and carved wooden ceilings of the Palazzo Apostolico (Vatican) undertaken under Pio IV (1555-1559) and Gregory XIII (1572-1585). The study gives specific attention to technique, materials and payments, and analyses the manufacture and decoration of the ceilings in the Sale dei Paramenti and Camera della Falda focusing on the Florentine carpenters Zanobio di Mariotto and Ambrogio Bonazzini.
description: Opus Incertum, Vol 3 (2017)
Addendum to "Coherent Lagrangian vortices: The black holes of turbulence"
Creators: name: Haller, G.
name: Beron-Vera, F. J.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Atmospheric and Oceanic Physics (physics.ao-ph)
subject: General Relativity and Quantum Cosmology (gr-qc)
subject: Dynamical Systems (math.DS)
subject: FOS: Physical sciences
subject: FOS: Mathematics

description: In Haller and Beron-Vera (2013) we developed a variational principle for the detection of coherent Lagrangian vortex boundaries. The solutions of this variational principle turn out to be closed null-geodesics of the Lorentzian metric associated with a generalized Green-Lagrange strain tensor family. This metric interpretation implies a mathematical analogy between coherent Lagrangian vortex boundaries and photon spheres in general relativity. Here we give an improved discussion on this analogy.
description: Revised 27 June 2014
Digitale Lehre im Rahmen der Grundausbildung in MINT-Fächern an Hochschulen : Didaktische Integration von digitalen Medien und E-Learningsystemen in Lehrveranstaltungen : Tagungsband 2022
Creators: name: Liebscher, Eckhard
name: Hübl, Reinhold
name: Merker, Jochen
name: Wacker, Benjamin
Publisher: name: Universitäts- und Landesbibliothek Sachsen-Anhalt
Year: 2023
Subjects: subject: Digitale Lehre
subject: Grundausbildung in MINT-Fächern
subject: Hochschulausbildung
subject: DDC::3** Sozialwissenschaften::37* Bildung und Erziehung::378 Hochschulbildung

description: Nach fast 3 Jahren der Corona-Pandemie war es ein Anliegen des Workshops, sich einmal in Präsenz über die neuesten Entwicklungen in der digitalen Lehre in den MINT-Fächern auszutauschen. Das persönliche Aufeinandertreffen und der Erfahrungsaustausch der Kolleginnen und Kollegen von Hochschulen und Universitäten war für die Teilnehmerinnen und Teilnehmer eine große Bereicherung. In den letzten 2 Jahren wurden in den Lehreinrichtungen umfangreiche Anstrengungen zum Aufbau bzw. der Weiterentwicklung der digitalen Lehre unternommen. Die dazu auf dem Workshop präsentierten Lösungen und Erkenntnisse sind es wert, in diesem Band vorgestellt zu werden.
Human Neural Stem Cells Survive Long Term in the Midbrain of Dopamine-Depleted Monkeys After GDNF Overexpression and Project Neurites Toward an Appropriate Target
Creators: name: Dodiya, Hemraj B.
name: Leranth, Csaba
name: Snyder, Evan Y.
name: Wakeman, Dustin R.
name: Redmond, D. Eugene
name: Samulski, R. Jude
name: Teng, Yang D.
name: Sladek, John R.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2014

description: Transplanted multipotent human fetal neural stem cells (hfNSCs) significantly improved the function of parkinsonian monkeys in a prior study primarily by neuroprotection, with only 3%–5% of cells expressing a dopamine (DA) phenotype. In this paper, we sought to determine whether further manipulation of the neural microenvironment by overexpression of a developmentally critical molecule, glial cell-derived neurotrophic factor (GDNF), in the host striatum could enhance DA differentiation of hfNSCs injected into the substantia nigra and elicit growth of their axons to the GDNF-expressing target. hfNSCs were transplanted into the midbrain of 10 green monkeys exposed to 1-methyl-4-phenyl-1,2,3,6-tetrahydro-pyridine. GDNF was delivered concomitantly to the striatum via an adeno-associated virus serotype 5 vector, and the fate of grafted cells was assessed after 11 months. Donor cells remained predominantly within the midbrain at the injection site and sprouted numerous neurofilament-immunoreactive fibers that appeared to course rostrally toward the striatum in parallel with tyrosine hydroxylase-immunoreactive fibers from the host substantia nigra but did not mature into DA neurons. This work suggests that hfNSCs can generate neurons that project long fibers in the adult primate brain. However, in the absence of region-specific signals and despite GDNF overexpression, hfNSCs did not differentiate into mature DA neurons in large numbers. It is encouraging, however, that the adult primate brain appeared to retain axonal guidance cues. We believe that transplantation of stem cells, specifically instructed ex vivo to yield DA neurons, could lead to reconstruction of some portion of the nigrostriatal pathway and prove beneficial for the parkinsonian condition.
Transparent Helium in Stripped Envelope Supernovae
Creators: name: Piro, Anthony L.
name: Morozova, Viktoriya S.
Publisher: name: arXiv
Year: 2014
Subjects: subject: High Energy Astrophysical Phenomena (astro-ph.HE)
subject: Solar and Stellar Astrophysics (astro-ph.SR)
subject: FOS: Physical sciences

description: Using simple arguments based on photometric light curves and velocity evolution, we propose that some stripped envelope supernovae (SNe) show signs that a significant fraction of their helium is effectively transparent. The main pieces of evidence are the relatively low velocities with little velocity evolution, as are expected deep inside an exploding star, along with temperatures that are too low to ionize helium. This means that the helium should not contribute to the shaping of the main SN light curve, and thus the total helium mass may be difficult to measure from simple light curve modeling. Conversely, such modeling may be more useful for constraining the mass of the carbon/oxygen core of the SN progenitor. Other stripped envelope SNe show higher velocities and larger velocity gradients, which require an additional opacity source (perhaps the mixing of heavier elements or radioactive nickel) to prevent the helium from being transparent. We discuss ways in which similar analysis can provide insights into the differences and similarities between SNe Ib and Ic, which will lead to a better understanding of their respective formation mechanisms.
description: Revised version accepted for publication in The Astrophysical Journal Letters, 4 pages, 4 figures
Adult Lung Spheroid Cells Contain Progenitor Cells and Mediate Regeneration in Rodents With Bleomycin-Induced Pulmonary Fibrosis: Lung Spheroid Cells for Lung Regeneration
Creators: name: Henry, Eric
name: Allen, Tyler
name: Anthony, Shirena
name: de Andrade, James B.M.
name: Hensley, M. Taylor
name: Cores, Jhon
name: Vandergriff, Adam
name: Cheng, Ke
name: Lobo, Leonard J.
name: Caranasos, Thomas G.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2015

description: Lung diseases are devastating conditions and ranked as one of the top five causes of mortality worldwide according to the World Health Organization. Stem cell therapy is a promising strategy for lung regeneration. Previous animal and clinical studies have focused on the use of mesenchymal stem cells (from other parts of the body) for lung regenerative therapies. We report a rapid and robust method to generate therapeutic resident lung progenitors from adult lung tissues. Outgrowth cells from healthy lung tissue explants are self-aggregated into three-dimensional lung spheroids in a suspension culture. Without antigenic sorting, the lung spheroids recapitulate the stem cell niche and contain a natural mixture of lung stem cells and supporting cells. In vitro, lung spheroid cells can be expanded to a large quantity and can form alveoli-like structures and acquire mature lung epithelial phenotypes. In severe combined immunodeficiency mice with bleomycin-induced pulmonary fibrosis, intravenous injection of human lung spheroid cells inhibited apoptosis, fibrosis, and infiltration but promoted angiogenesis. In a syngeneic rat model of pulmonary fibrosis, lung spheroid cells outperformed adipose-derived mesenchymal stem cells in reducing fibrotic thickening and infiltration. Previously, lung spheroid cells (the spheroid model) had only been used to study lung cancer cells. Our data suggest that lung spheroids and lung spheroid cells from healthy lung tissues are excellent sources of regenerative lung cells for therapeutic lung regeneration.
Multipeak Negative Differential Resistance from Interplay between Nonlinear Stark Effect and Double-Branch Current Flow
Creators: name: Sadek, Mikolaj
name: Wierzbowska, Malgorzata
name: Rode, Michal F.
name: Sobolewski, Andrzej L.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Mesoscale and Nanoscale Physics (cond-mat.mes-hall)
subject: FOS: Physical sciences

description: Multipeak negative differential resistance (NDR) molecular devices are designed from first principles. The effect of NDR is associated with the non-linear Stark shifts and the electron localization within the conductive region and contacts. Deep I(V)-curve well is formed when the aromatic molecule, containing intramolecular hydrogen bond, is connected to each lead by the double-branch contacts. This effect occurs at the same voltage where a single-junction case exhibits only a flat step in the current characteristics. The multipeak oscillations arise from the mutual effect of the Stark shifts located at the electron-rich contacts and parts of the molecule - this opens the route for further tailoring the desired properties.
description: 9 pages, 5 figures
I soffitti a cassettoni nei palazzi della famiglia Cesi
Creators: name: Nocchi, Livia
Publisher: name: Opus Incertum
Year: 2018

description: In the genre of monumental wood carved coffered ceilings some of the most significant examples can be seen in the palaces of the Cesi family, built between the seventh and ninth decades of the 16th century in Rome and in their country homes in Umbria and Lazio. The ceilings of the Acquasparta palace, probably designed by Giovan Domenico Bianchi, are great quality expressions of Roman artistic taste and their magnificence fully reflects the celebratory intention of the clients and their taste for this kind of decoration. In the case of the ceilings of Cantalupo in Sabina, some still in operation, we have detailed documentation on the sculptors and carpenters involved, including Guglielmo Della Porta and the French Stefano Possenti. As for the palazzo Armellini-Cesi in Via della Conciliazione there are two sumptuous ceilings whose decoration recalls typical models of the first half of the century. The Cesi always invested a great deal of resources in their residences for the implementation of imposing decorative systems to enhance the wealth and power of the family in Rome and the study of these ceilings and of the artists involved helps to understand the dynamics of work, of some models and their diffusion.
description: Opus Incertum, Vol 3 (2017)
Effective interaction approach to the Fermi hard-sphere system
Creators: name: Mecca, Angela
name: Lovato, Alessandro
name: Benhar, Omar
name: Polls, Artur
Publisher: name: arXiv
Year: 2014
Subjects: subject: Nuclear Theory (nucl-th)
subject: Quantum Gases (cond-mat.quant-gas)
subject: FOS: Physical sciences

description: The formalism based on correlated basis functions and the cluster expansion technique has been recently employed to derive an effective interaction from a realistic nuclear hamiltonian. To gauge the reliability of this scheme, we perform a systematic comparison between the results of its application to the Fermi hard-sphere system and the predictions obtained from low-density expansions, as well as from other many-body techniques. The analysis of a variety of properties, including the ground state energy, the effective mass and the momentum distribution, shows that the effective interaction approach is remarkably accurate, thus suggesting that it may be employed to achieve a consistent description of the structure and dynamics of nuclear matter in the density region relevant to astrophysical applications.
description: 10 pages, 13 figures
Our Fat Future: Translating Adipose Stem Cell Therapy
Creators: name: Nordberg, Rachel C.
name: Loboa, Elizabeth G.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2015

description: Human adipose stem cells (hASCs) have the potential to treat patients with a variety of clinical conditions. Recent advancements in translational research, regulatory policy, and industry have positioned hASCs on the threshold of clinical translation. We discuss the progress and challenges of bringing adipose stem cell therapy into mainstream clinical use.
I soffitti lignei del palazzo di Cosimo dei Medici a Milano e alcuni esempi di diffusione dei lacunari all’antica in Lombardia
Creators: name: Gritti, Jessica
Publisher: name: Opus Incertum
Year: 2018

description: The remains of the house owned by Cosimo Medici in Milan – a building given by Francesco Sforza to the Medicis in 1455 and reconstructed to become the office of the Medici Bank in the city – does not permit to undestand the rich presence of wooden ceilings. The description by Filarete and some 19th century sources, before the complete transformation of the house, show us an image of the coffered ceiling of the main room on the first floor, and the carpentry used for arcades and loggias in the courtyard. The decoration of the wooden ceiling of the main room seems to have been very well known in Milan during the second half of the 15th century, which explains why Galeazzo Maria Sforza wanted the wooden ceiling of his own chapel in the church of St. Celso in 1473 with coffers similar to those of two important models, the Medici bank and the chapel of the Santissima Annunziata in Florence. The essay illustrates the sources concerning the wooden ceilings of the Medici bank and some examples of their diffusion in Lombardy during the second half of the 15th century.
description: Opus Incertum, Vol 3 (2017)
D5.4 Workflows for the EnMS.
Creators: name: Capuzzi, Stefano
name: Caboni, Omar
name: Ferrai, Matteo
name: Lovern, Andrew
name: Stuart, Jay
Publisher: name: Zenodo
Year: 2023

description: The goal of this task will be to develop and implement the workflows for the EnMS. Scope of the work is to identify the energy sources, the energy use and related significant energy uses (i.e. heating, cooling, lighting, services, etc.) of each selected pilot, together with estimation of all possible improvement opportunities, including RES generation and CO2 emission reduction. This task will be performed based on outputs produced by all Tasks in WP2 and models developed in WP3 and WP4. Outputs produced by this Task will be the baseline for the impact energy saving assessment in WP7.
“Con una disinvoltura davvero fantastica”. Sul soffitto cassettonato dello studiolo di Gubbio
Creators: name: Belardi, Paolo
name: Ramaccini, Giovanna
Publisher: name: Opus Incertum
Year: 2018

description: Both commissioned by Duke Federico da Montefeltro, the Urbino Studiolo (executed between 1473 and 1476) and the Gubbio Studiolo (executed between 1479 and 1482 and kept in Gallery 501 at the Metropolitan Museum in New York since 1996) are so similar to seem identical, even if only in appearance. By focusing on the decorative apparatus, in fact, the presence of hallmarks is evident. In this sense a decisive role is played by the analysis of the coffered finishing that, being present both in Urbino and Gubbio, reveals substantial differences (mainly due to the variety in the spatial organisation). Although it regards a delimited part, the analysis of the Gubbio Studiolo (from compositional, constructive and material points of view), underlines the integration between scientific aspects (such as the careful geometrical planning) and artistic ones (such as the introduction of illusionistic devices), that perfectly summarise the principles of mathematical humanism advocated by the Montefeltro court.
description: Opus Incertum, Vol 3 (2017)