title: ÐÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸Ðµ ÑÑÐ°Ð¼Ð¼Ð° Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹ Saccharomyces cerevisiae, Ð¾Ð±Ð»Ð°Ð´Ð°ÑÑÐµÐ³Ð¾ Ð²ÑÑÐ¾ÐºÐ¸Ð¼ ÑÑÐ¾Ð²Ð½ÐµÐ¼ Ð¼ÑÑÐ°Ð³ÐµÐ½Ð½Ð¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸, Ð´Ð»Ñ Ð¿Ð¾Ð¸ÑÐºÐ° Ð¸ Ð¸Ð´ÐµÐ½ÑÐ¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¸ Ð¿ÑÐ¾Ð´ÑÑÐµÐ½ÑÐ¾Ð² Ð°Ð¼Ð¸Ð»Ð¾Ð»Ð¸ÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÐµÑÐ¼ÐµÐ½ÑÐ¾Ð²
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: The use of the yeast strain Saccharomyces cerevisiae, which has a high level of mutagenic activity, for the search and identification of producers of amylolytic enzymes
Creators: name: ÐÐ°ÑÑÐµÐ³ÑÐ½, ÐÑÑÐ°Ðº
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2023
Subjects: Ð°Ð»ÑÑÐ°-Ð°Ð¼Ð¸Ð»Ð°Ð·Ð°
saccharomyces cerevisiae
pahbah
ÑÑ-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐ¹ Ð¼ÑÑÐ°Ð³ÐµÐ½ÐµÐ·
Ð¼Ð¾Ð´Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ñ ÑÑÐ°Ð¼Ð¼Ð°
alpha-amylase
uv-induced mutagenesis
strain modification

description: Ð Ð°Ð±Ð¾ÑÐ° Ð¿Ð¾ÑÐ²ÑÑÐµÐ½Ð° ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐµ Ð±ÐµÐ·Ð¾Ð¿Ð°ÑÐ½Ð¾Ð³Ð¾ Ð²ÑÑÐ¾ÐºÐ¾ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ Ð¿ÑÐ¾Ð´ÑÑÐµÐ½ÑÐ° Ð°Ð¼Ð¸Ð»Ð¾Ð»Ð¸ÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÐµÑÐ¼ÐµÐ½ÑÐ¾Ð² Ð´Ð»Ñ Ð¿ÑÐ¸Ð¼ÐµÐ½ÐµÐ½Ð¸Ñ Ð² Ð¿ÑÐ¾Ð¼ÑÑÐ»ÐµÐ½Ð½ÑÑ Ð¿ÑÐ¾Ð¸Ð·Ð²Ð¾Ð´ÑÑÐ²Ð°Ñ. Ð Ð°Ð±Ð¾ÑÐ° Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð° Ð½Ð° Ð±Ð°Ð·Ðµ ÐÐ°Ð±Ð¾ÑÐ°ÑÐ¾ÑÐ¸Ð¹ Ð±Ð¸Ð¾ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ð¸ Ð¸ Ð³ÐµÐ½ÐµÑÐ¸ÐºÐ¸ ÑÑÐºÐ°ÑÐ¸Ð¾Ñ ÐÐÐ¦ Â«ÐÑÑÑÐ°ÑÐ¾Ð²ÑÐºÐ¸Ð¹ Ð¸Ð½ÑÑÐ¸ÑÑÑÂ» - ÐÐÐ¯Ð¤. Ð Ð°Ð±Ð¾ÑÐ° Ð²ÐºÐ»ÑÑÐ°Ð»Ð° ÑÑÐ¸ ÑÐµÑÐ¸Ð¸ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ¾Ð²: ÐÐµÑÐ²Ð°Ñ ÑÐ°ÑÑÑ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ° ÑÐ¾ÑÑÐ¾ÑÐ»Ð° Ð² Ð¿Ð¾Ð´Ð³Ð¾ÑÐ¾Ð²ÐºÐµ Ð¸ ÐºÑÐ»ÑÑÐ¸Ð²Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ð¸ ÐºÐ»ÐµÑÐ¾Ðº ÑÑÐ°Ð¼Ð¼Ð° Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹ Saccharomyces cerevisiae. ÐÐ¾ Ð²ÑÐ¾ÑÐ¾Ð¹ ÑÐ°ÑÑÐ¸ ÑÐºÑÐ¿ÐµÑÐ¸Ð¼ÐµÐ½ÑÐ° Ð¿ÑÐ¾Ð²Ð¾Ð´Ð¸Ð»ÑÑ ÑÐ°ÑÐ½Ð´ Ð£Ð¤-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð¼ÑÑÐ°Ð³ÐµÐ½ÐµÐ·Ð° ÑÑÐ°Ð¼Ð¼Ð°. Ð¢ÑÐµÑÑÑ ÑÐ°ÑÑÑ ÑÐ¾ÑÑÐ¾Ð¸Ñ Ð¸Ð· Ð¾Ð¿ÑÐµÐ´ÐµÐ»ÐµÐ½Ð¸Ñ ÑÐ´ÐµÐ»ÑÐ½Ð¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸ Ð°Ð»ÑÑÐ°-Ð°Ð¼Ð¸Ð»Ð°Ð·Ñ. Ð ÑÐ¾Ð´Ðµ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð½Ð¾Ð³Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¾ ÑÑÑÐ°Ð½Ð¾Ð²Ð»ÐµÐ½Ð¾, ÑÑÐ¾ Ð½Ð° ÐºÐ»ÐµÑÐºÐ¸ ÑÑÐ°Ð¼Ð¼Ð° Ð´ÑÐ¾Ð¶Ð¶ÐµÐ¹ Saccharomyces cerevisiae ÑÐ¾ Ð²ÑÑÑÐ¾ÐµÐ½Ð½ÑÐ¼ Ð³ÐµÐ½Ð¾Ð¼ Ð°Ð»ÑÑÐ°-Ð°Ð¼Ð¸Ð»Ð°Ð·Ñ Ð¿ÑÐ¸ Ð¿Ð¾Ð¿ÑÑÐºÐµ Ð¿ÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¸Ñ Ð¾Ð´Ð½Ð¾Ð³Ð¾ ÑÐ°ÑÐ½Ð´Ð° Ð£Ð¤-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð¼ÑÑÐ°Ð³ÐµÐ½ÐµÐ·Ð° Ð½Ðµ Ð±ÑÐ»Ð¾ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ð¾ ÑÐ¾ÑÑÐ° ÑÐµÑÐ¼ÐµÐ½ÑÐ°ÑÐ¸Ð²Ð½Ð¾Ð¹ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸. ÐÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð½Ð¾Ðµ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð¸Ð¼ÐµÐµÑ Ð¿ÐµÑÑÐ¿ÐµÐºÑÐ¸Ð²Ð½Ð¾Ðµ Ð½Ð°ÑÑÐ½Ð¾Ðµ Ð½Ð°Ð¿ÑÐ°Ð²Ð»ÐµÐ½Ð¸Ðµ Ð¸ Ð¼Ð¾Ð¶ÐµÑ Ð¿ÑÐ¸Ð²ÐµÑÑÐ¸ Ðº Ð¿Ð¾Ð»ÑÑÐµÐ½Ð¸Ñ Ð¿ÑÐ°ÐºÑÐ¸ÑÐµÑÐºÐ¸ Ð¿Ð¾Ð»ÐµÐ·Ð½ÑÑ ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐ¾Ð².
description: The work is devoted to the development of a safe, highly effective producer of amylolytic enzymes for use in industrial production. The work was carried out on the basis of the Laboratories of Biotechnology and Genetics of Eukaryotes of the Kurchatov Institute Research Center - PNPI. The work included three series of experiments: The first part of the experiment consisted in culturing cells of the yeast strain Saccharomyces cerevisiae. In the second part of the experiment, a round of UV-induced mutagenesis of the strain was carried out. The third part consists of determining the specific activity of alpha-amylase. In the course of the research, it was found that no growth of enzymatic activity was detected on cells of the yeast strain Saccharomyces cerevisiae with an embedded alpha-amylase gene when attempting one round of UV-induced mutagenesis. The conducted research has a promising scientific direction and can lead to practically useful results.