Additional file 1 of Nanoscale engineering of gold nanostars for enhanced photoacoustic imaging
Creators: name: Zhang, Rui
name: Thoröe-Boveleth, Sven
name: Chigrin, Dmitry N.
name: Kiessling, Fabian
name: Lammers, Twan
name: Pallares, Roger M.
Publisher: name: figshare
Year: 2024
Subjects: Biophysics
Biochemistry
Space Science
Medicine
Cell Biology
Molecular Biology
Biotechnology
Chemical Sciences not elsewhere classified

description: Supplementary Material 1: Figure S1. Distributions of root width of AuNS HEPES, EPPS and MOPS. Table S1. Morphological features of AuNS HEPES, EPPS and MOPS. Table S2. Branch characteristics of AuNS HEPES. Table S3. Branch characteristics of AuNS EPPS. Table S4. Branch characteristics of AuNS MOPS. Figure S2. Functionalization of AuNPs with the different ligands. (a, e, i, m) Hydrodynamic diameter (HD), (b, f, j, n) polydispersity index (PDI), (c, g, k, o) zeta potential and (d, h, l, p) extinction spectra of AuNPs functionalized with 2 kDa PEG, 6 kDa PEG, chitosan and melanin. Figure S3. Functionalization of AuNS HEPES with the different ligands. (a, e, i, m) Hydrodynamic diameter (HD), (b, f, j, n) polydispersity index (PDI), (c, g, k, o) zeta potential and (d, h, l, p) extinction spectra of AuNS HEPES functionalized with 2 kDa PEG, 6 kDa PEG, chitosan and melanin. Figure S4. TEM micrographs of AuNPs (a, f) before and after functionalization with (b, g) 2 kDa PEG, (c, h) 6 kDa PEG, (d, i) chitosan and (e, j) melanin. Figure S5. TEM micrographs of AuNS HEPES. TEM micrographs of AuNS (a, f) before and after functionalization with (b, g) 2 kDa PEG, (c, h) 6 kDa PEG, (d, i) chitosan and (e, j) melanin. Table S5. Optimal functionalization conditions and characterization of AuNPs. Table S6. Optimal functionalization conditions and characterization of AuNS HEPES. Figure S6. FTIR spectra of functionalized AuNPs and AuNS with (a) 2 kDa PEG, (b) 6 kDA PEG, (c) chitosan and (d) melanin. Figure S7. Phantoms used for PA imaging characterization. (a) Homemade gelatin phantom, and (b) low density polyethylene tubes used for the photoacoustic experiments before and after nanoparticle loading. Figure S8. Signal-to-noise ratios (SNRs) of PA intensities of AuNPs and AuNS. SNRs of PA intensities at 710 nm of (a) AuNPs, (b) AuNS HEPES, (c) AuNS EPPS, and (d) AuNS MOPS at different gold concentrations (from 0 to 200 mM). Figure S9. SNRs of PA intensities of functionalized AuNPs and AuNS. SNRs of PA intensities at 710 nm of functionalized (a) AuNPs, (b) AuNS HEPES, (c) AuNS EPPS, and (d) AuNS MOPS at gold concentrations of 200 mM. Table S7. Variation coefficients of PA intensities at 710 nm of (a) AuNPs, (b) AuNS HEPES, (c) AuNS EPPS and (d) AuNS MOPS at gold concentrations of 200 mM gold in polyethylene tubes. Figure S10. Cytotoxicity of AuNPs and AuNS. Cell viability of HEK 293 cells after 24 hours of incubation with (a) AuNPs, (b) AuNS HEPES, (c) AuNS EPPS and (d) AuNS MOPS (from 0 to 200 mM of gold). Figure S11. Stability of melanin-coated AuNS in 10 % fetal bovine serum (FBS). Hydrodynamic diameter (HD) of (a) melanin-coated AuNS HEPES, (b) EPPS and (c) MOPS in 10 % FBS at different incubation times. Figure S12. SNRs of PA intensities of melanin-coated AuNPs and AuNS in ex vivo imaging. SNRs of PA intensities at 710 nm of melanin-coated (a) AuNPs, (b) AuNS HEPES, (c) AuNS EPPS and (d) AuNS MOPS intramuscularly injected (50 mL, 0 or 200 mM gold) in the legs of deceased mice