Comparative analysis of <i>Klebsiella pneumoniae</i> strains isolated in 2012–2016 that differ by antibiotic resistance genes and virulence genes profiles
Creators: name: Lev, Anastasia I.
name: Astashkin, Eugeny I.
name: Kislichkina, Angelina A.
name: Solovieva, Ekaterina V.
name: Kombarova, Tatiana I.
name: Korobova, Olga V.
name: Ershova, Olga N.
name: Alexandrova, Irina A.
name: Malikov, Vladimir E.
name: Bogun, Alexander G.
name: Borzilov, Alexander I.
name: Volozhantsev, Nikolay V.
name: Svetoch, Edward A.
name: Fursova, Nadezhda K.
Publisher: name: Taylor & Francis
Year: 2018
Subjects: subject: Space Science
subject: Medicine
subject: Microbiology
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Genetics
subject: Biotechnology
subject: 59999 Environmental Sciences not elsewhere classified
subject: FOS: Earth and related environmental sciences
subject: FOS: Earth and related environmental sciences
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: FOS: Chemical sciences
subject: 69999 Biological Sciences not elsewhere classified
subject: Cancer
subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: FOS: Health sciences
subject: 60506 Virology
subject: Computational Biology

description: The antibacterial resistance and virulence genotypes and phenotypes of 148 non-duplicate <i>Klebsiella pneumoniae</i> strains collected from 112 patients in Moscow hospitals in 2012–2016 including isolates from the respiratory system (57%), urine (30%), wounds (5%), cerebrospinal fluid (4%), blood (3%), and rectal swab (1%) were determined. The majority (98%) were multidrug resistant (MDR) strains carrying <i>bla</i><sub>SHV</sub> (91%), <i>bla</i><sub>CTX-M</sub> (74%), <i>bla</i><sub>TEM</sub> (51%), <i>bla</i><sub>OXA</sub> (38%), and <i>bla</i><sub>NDM</sub> (1%) beta-lactamase genes, class 1 integrons (38%), and the porin protein gene <i>ompK36</i> (96%). The beta-lactamase genes <i>bla</i><sub>TEM-1</sub>, <i>bla</i><sub>SHV-1</sub>, <i>bla</i><sub>SHV-11</sub>, <i>bla</i><sub>SHV-110</sub>, <i>bla</i><sub>SHV-190</sub>, <i>bla</i><sub>CTX-M-15</sub>, <i>bla</i><sub>CTX-M-3</sub>, <i>bla</i><sub>CTX-M-55</sub>, <i>bla</i><sub>OXA-48</sub>, <i>bla</i><sub>OXA-244</sub>, and <i>bla</i><sub>NDM-1</sub> were detected; class 1 integron gene cassette arrays (<i>aadA1</i>), (<i>dfrA7</i>), (<i>dfrA1-orfC</i>), (<i>aadB-aadA1</i>), (<i>dfrA17-aadA5</i>), and (<i>dfrA12-orfF-aadA2</i>) were identified. Twenty-two (15%) of clinical <i>K. pneumoniae</i> strains had hypermucoviscous (HV) phenotype defined as string test positive. The <i>rmpA</i> gene associated with HV phenotype was detected in 24% of strains. The intrapersonal mutation of <i>rmpA</i> gene (deletion of one nucleotide at the polyG tract) was a reason for negative hypermucoviscosity phenotype and low virulence of <i>rmpA</i>-positive <i>K. pneumoniae</i> strain KPB584. Eighteen virulent for mice strains with LD<sub>50</sub> ≤ 10<sup>4</sup> CFU were attributed to sequence types ST23, ST86, ST218, ST65, ST2174, and ST2280 and to capsular types K1, K2, and K57. This study is the first report about hypervirulent <i>K. pneumoniae</i> strain KPB2580-14 of ST23<sup>K1</sup> harboring extended-spectrum beta-lactamase CTX-M-15 and carbapenemase OXA-48 genes located on pCTX-M-15-like and pOXA-48-like plasmids correspondingly.