Histones link inflammation and thrombosis through the induction of Weibel-Palade Body exocytosis
Creators: name: James, Paula D
Publisher: name: figshare
Year: 2021
Subjects: subject: 111501 Basic Pharmacology
subject: FOS: Clinical medicine

description: Damage-associated molecular patterns (DAMPs), including molecules like DNA and histones, are released into the blood following cell death. DAMPs promote a procoagulant phenotype through enhancement of thrombin generation and platelet activation thereby contributing to immunothrombosis. Weibel-Palade bodies (WPBs) are dynamic endothelial cell organelles that contain procoagulant and proinflammatory mediators, such as von Willebrand factor (VWF) and are released in response to cell stresses. VWF mediates platelet adhesion and aggregation, and has been implicated as a procoagulant component of the innate immune response. Objective: To determine the influence of histones and DNA on WPB release and characterize their association in models of inflammation. Methods: We treated C57BL/6J mice and cultured endothelial cells with histones (unfractionated, lysine or arginine-rich) and measured WPB exocytosis. We used inhibitors to determine a mechanism of histone-induced WPB release in vitro. We characterized the release of DAMPs and WPBs in response to acute and chronic inflammation in human and murine models. Results and conclusions: Histones, but not DNA, induced the release of VWF (1.46-fold) from WBPs and caused thrombocytopenia (0.74-fold), which impaired arterial thrombus formation in mice. Histones induced WPB release from endothelial cells in a caspase, calcium and charge-dependent manner and promoted platelet capture in a flow chamber model of VWF-platelet string formation. DAMPs and WPB-released proteins were elevated during inflammation and were positively correlated in chronic inflammation. These studies showed that DAMPs can regulate VWF levels and function by inducing its release from endothelial WPBs. This DAMPs-WPB axis may propagate immunothrombosis associated with inflammation.<br>https://amazonsale.io/eportfolios/1098/CircadiYin_Amazon/CircadiYin_Amazonhttps://amazonsale.io/eportfolios/1099/CircadiYin_Amazon_UK/CircadiYin_Amazon_UKhttps://amazonsale.io/eportfolios/1100/CircadiYin_Ingredients/CircadiYin_Ingredientshttps://amazonsale.io/eportfolios/1101/CircadiYin_Ingredients_List/CircadiYin_Ingredients_Listhttps://amazonsale.io/eportfolios/1102/CircadiYin_UK/CircadiYin_UKhttps://amazonsale.io/eportfolios/1103/CircadiYin_Australia/CircadiYin_Australiahttps://amazonsale.io/eportfolios/1104/CircadiYin_Canada/CircadiYin_Canadahttps://amazonsale.io/eportfolios/1105/CircadiYin_NZ/CircadiYin_NZhttps://amazonsale.io/eportfolios/1106/CircadiYin_South_Africa/CircadiYin_South_Africahttps://amazonsale.io/eportfolios/1107/CircadiYin_Walmart/CircadiYin_Walmarthttps://amazonsale.io/eportfolios/1108/CircadiYin_Reviews_UK/CircadiYin_Reviews_UKhttps://amazonsale.io/eportfolios/1109/CircadiYin_Reddit_Reviews/CircadiYin_Reddit_Reviewshttps://amazonsale.io/eportfolios/1110/CircadiYin_Review_Australia/CircadiYin_Review_Australiahttps://amazonsale.io/eportfolios/1111/CircadiYin_Supplement_Ingredients/CircadiYin_Supplement_Ingredientshttps://amazonsale.io/eportfolios/1112/LeptoFix_Amazon/LeptoFix_Amazonhttps://amazonsale.io/eportfolios/1113/LeptoFix_Amazon_UK/LeptoFix_Amazon_UKhttps://amazonsale.io/eportfolios/1114/LeptoFix_Ingredients/LeptoFix_Ingredients