Additional file 1 of Extreme drought triggers parallel shifts in wood anatomical and physiological traits in upper treeline of the Mediterranean Andes
Creators: name: Santini, Luiz
name: Craven, Dylan
name: Rodriguez, Daigard Ricardo Ortega
name: Quintilhan, Manolo Trindade
name: Gibson-Carpintero, Stephanie
name: Torres, Cristina Aravena
name: Roig, Fidel A.
name: Muñoz, Ariel A.
name: Venegas-Gonzalez, Alejandro
Publisher: name: figshare
Year: 2024
Subjects: subject: Ecology
subject: FOS: Biological sciences
subject: Plant Biology

description: Additional file 1: Fig. S1. Measurements of length and width of inter-vessel pits and their apertures of Kageneckia angustifolia, using different zoom x. (A) 3,000x, (B) 9,000x, (C) 11,000x, and (D) 19,000x. The pit total area and pit aperture area were calculated as π×Li×Wi/4. Fig. S2. Mean index residual chronologies (black series), numbers of series (light gray series) and EPS values (dashed line shows the arbitrary 0.85 EPS threshold) of Kageneckia angustifolia for (A) North, (B) Central, and (C) South populations. All sites had high mean sensitivity (&gt; 0.39) and expressed population signal values (&gt; 0.85) (Table 1). Fig. S3. Shifts in wood anatomical and physiological traits of K. angustifolia populations with tree size. Solid lines indicate statistically significant fits (p &lt; 0.05). Lines and 95% confidence intervals were fitted using linear mixed-effects models. (A) Ring width (mm yr−1); (B) vessel frequency (VF, ind mm−2); (C) hydraulic diameter (DH, µm); (D) vulnerability index of the xylem to embolism (VI); (E) potential hydraulic conductivity (Kp, kg m MPa−1 s−1); (F) wood intrinsic water-use efficiency (iWUE; μmol CO2 mol−1 H2O). Differences between periods for each site were evaluated using Fisher's LSD test (p &lt; 0.05). Fig. S4. Estimated values of wood anatomical and physiological traits of K. angustifolia populations across study sites and among periods. Lines and 95% confidence intervals were fitted using linear mixed-effects models. (A) Ring width (mm yr−1); (B) vessel frequency (VF, ind mm−2); (C) hydraulic diameter (DH, µm); (D) vulnerability index of the xylem to embolism (VI); (E) potential hydraulic conductivity (Kp, kg m MPa−1 s−1); (F) wood intrinsic water-use efficiency (iWUE; μmol CO2 mol−1 H2O). Differences between periods for each site were evaluated using Fisher's LSD test (p &lt; 0.05). Fig. S5. Boxplot of (A) carbon isotope discrimination ∆13C (‰), and (B) Ci (ppm) of Kageneckia angustifolia trees. Differences between periods for each site were evaluated according to Fisher's LSD test (p &lt; 0.05). Fig. S6. Boxplot of length and width of inter-vessel pits and their apertures of studied Kageneckia angustifolia trees. (A) Pit length, (B) pit width, (C) pit aperture length, (D) pit aperture width. * Indicate significant differences between period with p &lt; 0.05. Table S1. Parameters of LMMs evaluating the effect of drought period, tree size (DBH), and their interaction, on wood anatomical and physiological traits of K. angustifolia. Parameters are shown for F value (statistic) and P value, marginal R-squared (Rm2) and conditional R-squared (Rc2). Significant P values are shown in * (&lt; 0.05). Vessel traits are ring width (RW), vessel frequency (VF), hydraulic diameter (DH), vulnerability index (VI) and potential specific stem conductivity (Kp); and wood water-use efficiency (iWUE), carbon isotope discrimination ∆13C (‰) and Ci (ppm) of as physiological characteristics. We also included tree identity nested in study site as a random effect. RW, Kp, VI, Ci, ∆13C and DBH were log-transformed prior to analysis to meet assumptions of normality.