Additional file 1 of NIR-II emissive AIEgen photosensitizers enable ultrasensitive imaging-guided surgery and phototherapy to fully inhibit orthotopic hepatic tumors
Creators: name: Jia, Ruizhen
name: Xu, Han
name: Wang, Chenlu
name: Su, Lichao
name: Jing, Jinpeng
name: Xu, Shuyu
name: Zhou, Yu
name: Sun, Wenjing
name: Song, Jibin
name: Chen, Xiaoyuan
name: Chen, Hongmin
Publisher: name: figshare
Year: 2021
Subjects: Physical Sciences not elsewhere classified
Cell Biology
Pharmacology
Chemical Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Cancer

description: Additional file 1: Figure S1. Synthetic route of the PTZ-TQ molecule. Figure S2. 1H NMR spectrum of 3 in CDCl3. Figure S3. 13C NMR spectrum of 3 in CDCl3. Figure S4. 1H NMR spectrum of 4 in DMSO-d6. Figure S5. 13C NMR spectrum of 4 in DMSO-d6. Figure S6. 1H NMR spectrum of 5 in DMSO-d6. Figure S7. 13C NMR spectrum of 5 in DMSO-d6. Figure S8. 1H NMR spectrum of 6 in DMSO-d6. Figure S9. 13C NMR spectrum of 6 in DMSO-d6. Figure S10. 1H NMR spectrum of PTZ-TQ in pyridine-d5. Figure S11. 13C NMR spectrum of PTZ-TQ in pyridine-d5. Figure S12. The mass spectrum of PTZ-TQ. m/z: calcd. 958.39, found: 959.39 for [M]+. Figure S13. The zeta potentials of PTZ-TQ and PTZ-TQ-AIE dots. Figure S14. The stability evaluation of PTZ-TQ-AIE dots in PBS based on hydrodynamic size. PTZ-TQ-AIE dots were incubated in PBS at different time points. The hydrodynamic size at 0 h, 2 h, 4 h, 8 h, 12 h, 24 h, 48 h and 72 h, had no obvious changes compared with 0 h. Figure S15. The stability evaluation of PTZ-TQ-AIE dots in DMEM based on hydrodynamic size (a-d). PTZ-TQ-AIE dots were incubated in DMEM at different time points. The hydrodynamic size at 0 h (a), 12 h (b), 24 h (c), 48 h (d) had no obvious changes compared with 0 h. Figure S16. The stability evaluation of PTZ-TQ-AIE dots in 5% FBS based on hydrodynamic size (a-d). PTZ-TQ-AIE dots were incubated in 5% FBS at different time points. The hydrodynamic size at 0 h (a), 12 h (b), 24 h (c), 48 h (d) had no obvious changes compared with 0 h. Figure S17. Fluorescence quantum yield measurements of PTZ-TQ-AIE dots in water. Absorbance and fluorescence spectra of IR26 in DEM (a-c), and PTZ-TQ-AIE dots in water (d-f). The integrated fluorescence was plotted against absorbance for both IR26 and fluorophores and fitted into a linear function, linear fit of IR26 (c) and PTZ-TQ-AIE dots (f). Figure S18. (a) ROS generation of PTZ-TQ-AIE dots with different concentrations. (b) ROS generation of PTZ-TQ-AIE dots with different times. The light source: 808 nm NIR laser (0.25 W/cm2) (unreal thread have no laser and real thread have laser). Figure S19. Decrease in absorbance of DPBF at 417 nm in the presence of PTZ-TQ-AIE dots (a) and ICG (b) as a function of irradiation time. Figure S20. ROS levels in HepG2 cells analyzed by flow cytometry. Figure S21. In vivo blood test including red blood cells, platelet, and white blood cell count of healthy mice injected with saline, PTZ-TQ-AIE dots for 1d and 7d. Figure S22. (a) The ex vivo biodistribution analysis of PTZ-TQ-AIE dots in BABL/c normal mice at 168 h under an 808 nm laser excitation (1250 nm bandpass filter, 300 ms). (b) The ex vivo fluorescent signal of different organs. Figure S23. Blood circulation half-life curve of PTZ-TQ-AIE dots in mice. The circulation half-life was determined to be 61 minutes by fitting the data (5 min, 10 min, 20 min, 0.5 h, 1 h, 3 h, 6 h, 9 h, 12 h, 24 h, 30 h) to a first-order exponential decay (n=3). Figure S24. Ex vivo NIR-II fluorescent images of main organs collected after 2 h post injection. Figure S25. The quantitative analysis of fluorescence intensity of orthotropic liver tumor at different time points after tail vein injection of PTZ-TQ-AIE dots (0.2 mL, 0.5 mg/mL). Figure S26. (a) Signal-to-noise ratio (SNR) and (b) tumor-normal liver ratio of Figure 4c (n=3). Figure S27. (a) Pictures of tumor resection process. (b) H&amp;E staining of excised tumor and normal liver tissue (scale bar: 200 μm). Figure S28. (a) Bioluminescence imaging of orthotopic liver cancer mice treated with PBS, PBS+Laser, PTZ-TQ-AIE dots, PDT, Surgery, Surgery+PDT, PDT+Surgery (BT: Before PDT; AT: after PDT; BS: Before surgery; AS: after surgery). (b) body weight curves after different treatments (n=3). Figure S29. MR imaging of orthotopic liver cancer mice treated with PBS, PBS+Laser, PTZ-TQ-AIE dots, PDT, Surgery, Surgery+PDT, PDT+Surgery after therapy. Figure S30. H&amp;E staining of heart, liver, spleen, lung, and kidney tissue slices for different groups after treatments: (a) PBS, (b) PBS+Laser, (c) PTZ-TQ-AIE dots, (d) PDT, (e) Surgery, (f) Surgery+PDT, (g) PDT+Surgery.