MOESM4 of Comparative metabolic profiling of the lipid-producing green microalga Chlorella reveals that nitrogen and carbon metabolic pathways contribute to lipid metabolism
Creators: name: Chen, Hui
name: Yanli Zheng
name: Zhan, Jiao
name: Chenliu He
name: Wang, Qiang
Publisher: name: Figshare
Year: 2017
Subjects: subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Pharmacology
subject: 39999 Chemical Sciences not elsewhere classified
subject: FOS: Chemical sciences
subject: FOS: Chemical sciences
subject: Developmental Biology
subject: Cancer
subject: Computational Biology

description: Additional file 4: Figure S1. Lipid accumulation of 15 Chlorella strains grown in regular BG11 media at the stationary phase. 1. Chlorella sorokiniana C1; 2. Chlorella sp. C2; 3. Chlorella sorokiniana C3; 4. Chlorella sorokiniana C7; 5. Chlorella sp. A2; 6. FACHB1 (Chlorella luteorividis); 7, FACHB37 (Chlorella vulgaris); 8. FACHB960 (Chlorella sp.); 9. FACHB1068 (Chlorella vulgaris); 10. FACHB1216 (Chlorella pyrenoidosa); 11, FACHB1222 (Chlorella pyrenoidosa); 12. FACHB1227 (Chlorella vulgaris); 13. FACHB1552 (Chlorella sp.); 14. FACHB1568 (Chlorella sp.); 15. FACHB1580 (Chlorella sp.). FACHB1, FACHB37, FACHB960, FACHB1068, FACHB1216, FACHB1222, FACHB1227, FACHB1552, FACHB1568 and FACHB1580 were obtained from the Freshwater Algae Culture Collection of the Institute of Hydrobiology, Chinese Academy of Sciences. The expected lipid bands were marked using red box. The figure is representative of three replicated studies with similar findings. Figure S2. Physiological analysis of three Chlorella strains. A, growth curve of three Chlorella strains grown in regular BG11 medium; B, photos of three Chlorella strains grown in regular BG11 medium; C, total N content in culture medium during three Chlorella strains grown in regular BG11 medium; D, cell growth of three Chlorella strains following re-suspension in N- medium. All data points in the current and following figures represent the means and SD of three to five biological replicates (t test, p &lt; 0.05). Figure S3. Overall trends analysis of lipid accumulation in three Chlorella strains. A, lipid accumulation analyzed by TLC during 0-9 d under N- treatment; asterisk symbol, glyceryl trioleate as loading standard; the expected lipid bands for further clarity were marked using red box; the figure is representative of three replicated studies with similar findings. B, lipid quantification during 2-9 d under N- treatment by using ImageJ (ver1.41, NIH), and the significance of the differences between the 2 d and other test values at 3-9 d in each panel was tested using a one-way ANOVA. *, p &lt; 0.05. Figure S4. Lipid quantification of three Chlorella strains under N- treatment. Lipid content at 0 d, 1 d, 2 d and 6 d after N- treatment was determined according to FigureÂ 1B by using ImageJ (ver1.41, NIH), and the significance of the differences between the C1 and other two strains was tested using a one-way ANOVA. *, p &lt; 0.05. Figure S5. The global metabolic pathways of 220 detected compounds (black) and some undetected compounds (gray) of known identity in metabolome analysis in three Chlorella strains. Solid arrow, one step of metabolic flow; dotted arrow, more than one step of metabolic flow; blue dashed line box, the pathways related to nitrogen metabolism; red dashed line box, the pathways related to carbon metabolism. Figure S6. The relative enzyme activities verification of Chlamydomonas reinhardtii knock-out mutants deficient in glutamine synthetase (GS), glutamate synthase/NADH-dependent (NADH-GSN), glutamate synthase/Fd-dependent (Fd-GSN), aspartate aminotransferase (AST), alanine aminotransferase (ALT), pyruvate kinase (PK) and citrate synthase (CS). The control (background strain CC4533) value of each enzyme activity was set to 1 for easy comparison.