cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Generation of Induced Pluripotent Stem Cells from Patients with Duchenne Muscular Dystrophy and their induction to Neurons
Creators: name: Annese, Tiziana
name: Ruggieri, Simona
name: Viggiano, Luigi
name: Rubolino, Carmela
name: Gerbino, Andrea
name: Corsi, Patrizia
name: Tamma, Roberto
name: Ribatti, Domenico
name: Operto, Francesca
name: Margari, Lucia
name: Nico, Beatrice
Publisher: name: Firenze University Press
Year: 2017
Subjects: Duchenne Muscular Dystrophy; Induced Stem Cells

description: Duchenne muscular dystrophy (DMD) is an X-linked recessive disease characterized by deficient expression of the cytoskeletal protein dystrophin. DMD has been associated with intellectual disability and mental retardation (MR) and is present in about a third of all patients. Loss of Dp71, the major dystrophin-gene product in brain, and the dystrophin associated proteins (DAPs) are thought to contribute to severity of MR, but the specific function of the neural dystrophin proteins are poorly understood for a limited access to DMD patients brain tissue (1). Differentiation of induced Pluripotent Stem Cells (iPSCs) provides an opportunity to generate an unlimited supply of living neurons genetically identical to those present in patients. In this study we obtained DMD-iPSCs from peripheral blood mononuclear cells of DMD patients with cognitive impairment and we performed morphological (fluorescence and electron microscopy), molecular (Western Blot and Real Time PCR) and functional (electrophysiology) characterization both of iPSC-derived Neural Stem Cells (NSCs) and the differentiated neurons. Preliminary data showed a reduction of Dp71 and DAPs proteins, including the AQP4, potassium channel Kir4.1, α- and β-dystroglycan (α/βDG) and α-syntrophin (αSyn), both at transcriptional and traductional level, coupled with membrane dys-arrangment in DMD-iPSCs compared with healthy iPSCs. Moreover, we demonstrated that the neurons obtained from the differentiation of iPSCs derived from DMD patient showed after confocal analysis, altered cytoskeleton and reduction in Dp71expression, and by single-cell imaging experiments and electrophysiology, altered intracellular calcium homeostasis, in analogy with what shown in the dystrophic mdx mouse neurons (2). Overall these results showed that the Dp71 and DAPs alterations affect also the neural precursor as well as the differentiated neurons in DMD patients, so suggesting a key role in the pathogenesis of neurocognitive deficits in DMD disease.
description: Italian Journal of Anatomy and Embryology, Vol. 122, No. 1 (Supplement) 2017
Additional file 3 of Identification of transient receptor potential channel genes from the swimming crab, Portunus Trituberculatus, and their expression profiles under acute temperature stress
Creators: name: Qian, Yichen
name: Yu, Qiaoling
name: Zhang, Jun
name: Han, Yaoyao
name: Xie, Xi
name: Zhu, Dongfa
Publisher: name: figshare
Year: 2024
Subjects: subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Physiology
subject: Environmental Sciences not elsewhere classified
subject: Chemical Sciences not elsewhere classified
subject: Ecology
subject: Biological Sciences not elsewhere classified
subject: Cancer
subject: Inorganic Chemistry
subject: FOS: Chemical sciences

description: Supplementary Material 3: Full-length gels which have been cropped in the main text
An animal and cellular study on αB-crystallin activation in cardiac muscle by acute exercise
Creators: name: Antonioni, Ambra
name: Dimauro, Ivan
name: Mercatelli, Neri
name: Barone, Rosario
name: Macaluso, Filippo
name: Di Felice, Valentina
name: Caporossi, Daniela
Publisher: name: Firenze University Press
Year: 2017

description: Alpha-B-Crystallin (CRYAB), a Small Heat Shock Protein sensitive to oxidative stress, is expressed in many tissues and implicated in various biological processes. In cardiac muscle, CRYAB exerts a cardio protective role in ischemia-induced damage preventing apoptosis and necrosis. In the present study we used forty young (7-weeks old) healthy male mice (BALB/c AnNHsd), which after 1 week of acclimatization to the new housing environment, runned 2 days per 10 minutes. The TR mice ran for 60 min at a speed of 5.5 m/min. Mice were sacrificed immediately after, 15 and 120 minutes after the end of the acute bout of endurance exercise (TR-0’, TR-15’ and TR-120’, respectively). We prepared samples from the heart and from the group of posterior muscles study αB-crystallin’ response at different time of recovery from an acute aerobic exercise (1 hour), correlating its modulation with oxidative stress level. We found that a single bout exercise lead to a specific short-term increase of phospho-αB-crystallin level (pCRYAB), without changes of its total expression. Further, the level of 4-hydroxynonenal, a marker of lipidic peroxidation, has shown a similar trend of pCRYAB enhancement. This may indicate that CRYAB in cardiac muscle is activated and it has a putative role in oxidative stress during exercise. These results are supported by our previous data obtained in mouse skeletal tissues (i.e. gastrocnemius, soleus) and in H₂O₂-treated C2C12 myotubes. In particular we observed not only a fiber-dependent response of pCRYAB, but also its translocation into myofibrillar compartment. Experiments are in progress to further investigate on CRYAB role during exercise and its interactions with cytoskeletal structures.
description: Italian Journal of Anatomy and Embryology, Vol. 122, No. 1 (Supplement) 2017
Anatomical theatre place of Knowledge – the pivotal role of anatomist in its realization
Creators: name: Armocida, Emanuele
name: Ruggeri, Alessandro
Publisher: name: Firenze University Press
Year: 2017
Subjects: Anatomical theatres; Antonio Scarpa; history of anatomy; protecting cultural heritage; Thesa project

description: Human anatomy dissection represented a cornerstone in the evolution of medicine and modern scientific thought. The anatomical theatres, some of which are considered true masterpieces of architecture, are the place where concretely men learned to know themselves with a scientific method. Anatomists had a pivotal role in the buildings of anatomical theatres, using their knowledge in the research process of more functional architectures for demonstrative and experimental science. Antonio Scarpa (1752-1832) is an emblematic figure in this scenario. He studied anatomy at the University of Padua in the oldest permanent anatomical theatre of the world, originate in 1594 from a joint project conceived by Paolo Sarpi, scientist and church reformer, and Hieronymus Fabricius Ab Aquapendente, anatomist. In 1772, Scarpa became professor at the University of Modena. Bearing witness to the architectural value of the theatre in Padua is the fact that in 1774 Scarpa, involved in the planning for an anatomical theatre in Modena, had the professor of surgery in Padua, Girolamo Vandelli, send him a wooden model of the theatre there. Another project, less expensive, was selected instead. Later, in 1783, Scarpa was made professor of anatomy at the University of Pavia and promptly he promoted the building of an anatomical theatre there. The building was concluded in 1785, its semicircular layout is modeled on ancient theatres and the Palladian Olympic Theatre of Vicenza. Today, most of the anatomical theatres are lost or forgotten. The Thesa project will provide a census of anatomical theatres, both survived and not, which will allow us to identify connections among them, among the anatomists who studied there and the mutual influences that characterize their form. We believe the achievement of these objectives defines the essential conditions necessary to regain full awareness of the value of anatomical theatres in both the academic and popular contexts, thus creating a fertile cultural basis for new initiatives that can continue the quest for knowledge undertaken in the past in these places. From an architectural and evocative perspective, they are and will remain places where man puts himself at the centre and at the same time observes himself.
description: Italian Journal of Anatomy and Embryology, Vol. 122, No. 1 (Supplement) 2017
The Mediterranean shipwreck of April 18 2015: challenges in the postmortem examination of the victims
Creators: name: Asmundo, Alessio
name: Cattaneo, Cristina
name: Barbera, Nunziata
name: Argo, Antonella
name: Piscitelli, Vittorio
Publisher: name: Firenze University Press
Year: 2017
Subjects: Dead migrants; identification; biological profile; autopsy protocol

description: Background and Aims. On the 18th of April 2015, one of the largest shipwrecks in the Mediterranean sea occurred with around 800 gone missing. Among European indifference and inactivity, the Italian Government created a task force, through the will of the Commissioner’s Office of Missing Persons, the Italian Navy, the Prefecture of Siracusa, the Police, the Military Red Cross and the University of Milano flanked by the Universities of Catania, Palermo and Messina and other 10 Universities for the recovery and the identification of these victims in a challenging scenario where collection of post-mortem and ante-mortem data is very difficult respectively because of the conditions of the bodies and the political situation of the countries of provenance of the victims as well as the dispersal of their relatives and loved ones all over the world. According to the DVI protocols, identification relies mainly upon primary (genetic, fingerprint, teeth) criteria, but previous experience on the Lampedusa disaster has proven that such criteria may not guarantee high success rates. Personal descriptors of faces (ante-mortem photographs) are becoming more and more important. Materials and Methods. Since July 2015, 69 body bags bodies have been recovered around the wreck e and 458 body bags inside the boat; these were recovered by the Italian Navy and brought to a Naval area near Siracusa where a morgue was set up. Here PM examination on all bodies was performed and a biological profile was created through detailed pathological, anthropological odontological and radiological examination of the remains along with sampling for DNA analysis. 3D scans of the face or crania also were performed. Results. Preservation of the bodies varied from decomposed bodies, partial skeletonization of the extremities to complete skeletonization (with lack of the skull). Over 550 bodies were recovered along with many commingled remains. Preservation of bodies varied from partial skeletonization of the extremities (41%) to complete skeletonization (23%). All bodies so far belong to males. Conclusions. The humanitarian disposition of countries, politicians and scientists is a fundamental prerequisite for identifying victims of these disasters. Because of the difference in type of AM data available in such cases, autopsy procotols and identification strategies may need to vary.
description: Italian Journal of Anatomy and Embryology, Vol. 122, No. 1 (Supplement) 2017
Spheroids from human primary skin myofibroblasts as experimental system for myofibroblast deactivation studies
Creators: name: Avagliano, Angelica
name: Granato, Giuseppina
name: Ruocco, Maria R.
name: Masone, Stefania
name: Imparato, Valeria
name: Montagnani, Stefania
name: Arcucci, Alessandro
Publisher: name: Firenze University Press
Year: 2017
Subjects: Myofibroblasts, spheroids

description: Myofibroblasts are activated fibroblasts, involved in tissue repair and cancer, characterized by de novo expression of alpha smooth muscle actin (α-SMA), increased secretion of growth factors and immunoregulatory phenotype [1]. At the end of wound healing myofibroblasts undergo apoptotic cell death, whereas in vitro they are also subjected to a programmed necrosis-like cell death, termed nemosis, associated with cyclooxygenase-2 (COX-2) expression induction and inflammatory response [1,2]. Moreover, fibroblasts form clusters during wound healing, fibrotic states and tumorigenesis. In this investigation, we produced and analysed clusters such as spheroids from human primary cutaneous myofibroblasts to evaluate apoptotic or necrotic cell death, inflammation and activation markers during myofibroblasts clustering. The spheroids formation does not induce apoptosis, necrotic cell death and COX-2 protein induction. The significant decrease of α-SMA in protein extracts of spheroids, the anti- migratory effect of spheroid-conditioned medium on normal cell lines and the absence of proliferation marker Ki-67 after 72 h of three-dimensional culture indicated that myofibroblasts undergo a deactivation process within spheroids. The cells of spheroids, reverted to adhesion growth, preserve their proliferation capability and are able to reacquire a myofibroblastic phenotype. Furthermore, the spontaneous formation of clusters and spheroids on plastic and glass substrates suggests that aggregates formation could be a physiological feature of cutaneous myofibroblasts. This study represents an experimental model to analyse myofibroblasts deactivation and indicates that fibroblast clusters could be a cell reservoir regulating tissue turnover.
description: Italian Journal of Anatomy and Embryology, Vol. 122, No. 1 (Supplement) 2017
Modification of ZnO Electron Transport Layers with Benzoic Acid Derivatives in Non-fullerene-based Organic Solar Cells
Creators: name: LIU, HAO
Publisher: name: Monash University
Year: 2023
Subjects: classificationCode: 400910
subject: Photovoltaic devices (solar cells)

description: Electron transport layers play a significant role in the device performance and stability of organic solar cells, particularly those based on state-of-the art non-fullerene acceptors. This thesis aims to shed more light on this topic by exploring the use of carboxylic acid functionalized molecules (i.e. benzoic acid derivatives) as modifiers of the commonly used ZnO electron transport layer in polymer/non-fullerene acceptor organic solar cells. The results presented in this thesis establishes the effectiveness of this modification approach, and assesses the potential for further developments aimed at optimising similar organic solar cell configurations.
Additional file 1 of Analysis of narrative assessments of internal medicine resident performance: are there differences associated with gender or race and ethnicity?
Creators: name: Klein, Robin
name: Snyder, Erin D.
name: Koch, Jennifer
name: Volerman, Anna
name: Alba-Nguyen, Sarah
name: Julian, Katherine A.
name: Thompson, Vanessa
name: Ufere, Nneka N.
name: Burnett-Bowie, Sherri-Ann M.
name: Kumar, Anshul
name: White, Bobbie Ann A.
name: Park, Yoon Soo
name: Palamara, Kerri
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Cell Biology
subject: Biotechnology
subject: Environmental Sciences not elsewhere classified
subject: Sociology
subject: FOS: Sociology
subject: Cancer
subject: Mental Health
subject: Plant Biology
subject: FOS: Biological sciences

description: Supplementary Material 1
title: Analysis of the MultiConfiguration time-dependent Hartree-Fock equations
Creators: name: Trabelsi, Saber
Year: 2008

description: Die Multiconfigurationsmethoden sind eine natürliche Verbesserung der wohlbekannten einfachen Näherungen der linearen N-Teilchen Schrödinger Gleichung für Atom- und Molekül-systeme mit binärer Wechselwirkung, die realistischerweise mit dem Coulomb Potential modelliert wird. Modelle wie MCTDHF werden intensiv verwendet für numerische Simulationen in der Quantenphysik/ chemie. Die vorliegende Arbeit legt die ersten mathematisch rigorosen Fundamente der MCTDH(F) Gleichungen mit singulärer Coulomb-Wechselwirkung. Wir formulieren in einer für die mathematische Analysis besonders geeigneten Weise das assoziierte Anfangswertproblem, für das wirWohlgestelltheitsresultate zeigen abhängig von der Regularität der Anfangsdaten, mit und ohne Annahmen über den Rang der assoziierten Dichtematrix. Weiters präsentieren wir numerische Simulationen eines vereinfachten Models, mit besonderem Interesse an der so genannten Korrelation, welche eine der wesentlichen Motivationen und Vorteile der Multikonfigurationsmethoden ist im Vergleich zu Hartree-Fock Modellen.
description: The multiconfiguration methods are a natural improvement of well-known simple models for approximating the linear N body Schrödinger equation for atomic and molecular systems with binary - Coulomb in realistic situations- interactions, like the Hartree and the Hartree-Fock equation. Models like MCTDHF are intensively used for numerical simulations in quantum physics/chemistry. However, from the mathematical point of view, these equations are yet poorly understood. The present contribution gives the first rigorous mathematical foundation of the MCTHDH(F) equations with the singular Coulomb interaction. In particular, we formulate in a convenient way for the mathematical analysis the associated initial value problem for which we obtain well-posedness results depending on the regularity of the initial data, with and without an assumption on the rank of the associated density matrix. Also, numerical simulations of a toy model are presented with particular interest to the so called correlation which is one of the main motivations and advantage of the multiconfiguration methods compared to Hartree-Fock models.
Current challenges and future perspectives in oral absorption research. An opinion of the UNGAP network
Creators: name: Kuentz, Martin
name: Vinarov, Zahari
name: Bertil, Abrahamsson
name: Artursson, Per
name: Batchelor, Hannah
name: Berben, Philippe
name: Bernkop-Schnürch, Andreas
name: Butler, James
name: Ceulemans, Jens
name: Davies, Nigel
name: Dupont, Didier
name: Eide Flaten, Goril
name: Fotaki, Nikoleta
name: Jannin, Vincent
name: Keemink, Janneke
name: Kesisoglou, Filippos
name: Koziolek, Mirko
name: Augustijns, Patrick
name: Griffin, Brendan
Publisher: name: Elsevier
Year: 2021
Subjects: Specific patient populations
Regional differences
Advanced formulations
Amorphous solid dispersions
Lipid-based formulations
Food-drug interactions
Microbiome
In vitro tools
PBPK modeling

description: Although oral drug delivery is the preferred administration route and has been used for centuries, modern drug discovery and development pipelines challenge conventional formulation approaches and highlight the insufficient mechanistic understanding of processes critical to oral drug absorption. This review presents the opinion of UNGAP scientists on four key themes across the oral absorption landscape: (1) specific patient populations, (2) regional differences in the gastrointestinal tract, (3) advanced formulations and (4) food-drug interactions. The differences of oral absorption in pediatric and geriatric populations, the specific issues in colonic absorption, the formulation approaches for poorly water-soluble (small molecules) and poorly permeable (peptides, RNA etc.) drugs, as well as the vast realm of food effects, are some of the topics discussed in detail. The identified controversies and gaps in the current understanding of gastrointestinal absorption-related processes are used to create a roadmap for the future of oral drug absorption research.
Additional file 2 of Analysis of narrative assessments of internal medicine resident performance: are there differences associated with gender or race and ethnicity?
Creators: name: Klein, Robin
name: Snyder, Erin D.
name: Koch, Jennifer
name: Volerman, Anna
name: Alba-Nguyen, Sarah
name: Julian, Katherine A.
name: Thompson, Vanessa
name: Ufere, Nneka N.
name: Burnett-Bowie, Sherri-Ann M.
name: Kumar, Anshul
name: White, Bobbie Ann A.
name: Park, Yoon Soo
name: Palamara, Kerri
Publisher: name: figshare
Year: 2024
Subjects: subject: Medicine
subject: Cell Biology
subject: Biotechnology
subject: Environmental Sciences not elsewhere classified
subject: Sociology
subject: FOS: Sociology
subject: Cancer
subject: Mental Health
subject: Plant Biology
subject: FOS: Biological sciences

description: Supplementary Material 2
Analysis of bioavailability and induction of glutathione peroxidase by dietary nanoelemental, organic and inorganic selenium
Creators: name: Lenz, Markus
name: Ringuet, Mitchell
name: Hunne, Billie
name: Bravo, David
name: Furness, John
Publisher: name: MDPI
Year: 2021
Subjects: selenium
bioavailabity
nanoelemental selenium
animal feed
antioxydant enzymes

description: Dietary organic selenium (Se) is commonly utilized to increase formation of selenoproteins, including the major antioxidant protein, glutathione peroxidase (GPx). Inorganic Se salts, such as sodium selenite, are also incorporated into selenoproteins, and there is evidence that nanoelemental Se added to the diet may also be effective. We conducted two trials, the first investigated inorganic Se (selenite), organic Se (L-selenomethionine) and nanoelemental Se, in conventional mice. Their bioavailability and effectiveness to increase GPx activity were examined. The second trial focused on determining the mechanism by which dietary Se is incorporated into tissue, utilising both conventional and germ-free (GF) mice. Mice were fed a diet with minimal Se, 0.018 parts per million (ppm), and diets with Se supplementation, to achieve 0.07, 0.15, 0.3 and 1.7 ppm Se, for 5 weeks (first trial). Mass spectrometry, Western blotting and enzymatic assays were used to investigate bioavailability, protein levels and GPx activity in fresh frozen tissue (liver, ileum, plasma, muscle and feces) from the Se fed animals. Inorganic, organic and nanoelemental Se were all effectively incorporated into tissues. The high Se diet (1.7 ppm) resulted in the highest Se levels in all tissues and plasma, independent of the Se source. Interestingly, despite being ~11 to ~25 times less concentrated than the high Se, the lower Se diets (0.07; 0.15) resulted in comparably high Se levels in liver, ileum and plasma for all Se sources. GPx protein levels and enzyme activity were significantly increased by each diet, relative to control. We hypothesised that bacteria may be a vector for the conversion of nanoelemental Se, perhaps in exchange for S in sulphate metabolising bacteria. We therefore investigated Se incorporation from low sulphate diets and in GF mice. All forms of selenium were bioavailable and similarly significantly increased the antioxidant capability of GPx in the intestine and liver of GF mice and mice with sulphate free diets. Se from nanoelemental Se resulted in similar tissue levels to inorganic and organic sources in germ free mice. Thus, endogenous mechanisms, not dependent on bacteria, reduce nanoelemental Se to the metabolite selenide that is then converted to selenophosphate, synthesised to selenocysteine, and incorporated into selenoproteins. In particular, the similar efficacy of nanoelemental Se in comparison to organic Se in both trials is important in the view of the currently limited cheap sources of Se.
Digitalisierung/Automatisierung –Chancen, Risiken und gute Gestaltung
Creators: name: Brüngger, Jonas
Publisher: name: Fachhochschule Nordwestschweiz FHNW
Year: 2022
Subjects: Automatisierung
Digitalisierung

description: Inhalte der Präsentation: Begriffsbestimmung Digitalisierung/Automatisierung Ziele von Automatisierung Chancen von Automatisierung Risiken und Nebenwirkungen von Automatisierung Welche Automatisierungsstrategien gibt es? Kriterien für gute Automatisierung
Ponds and pondscapes for biodiversity
Creators: name: Biggs, Jeremy
name: Hoyle, Sarah
Publisher: name: Zenodo
Year: 2025

description: An information poster, designed for the general public, covering:

The critical role of ponds and pondscapes for biodiversity

Ponds as an opportunity for protecting freshwater biodiversity

This poster was created for the Horizon 2020 PONDERFUL project. It is one in a series of five information posters created for the project.
Datenmanagement an Kunsthochschulen - Anforderungen zwischen Forschung, Vermittlung und Archiv
Creators: name: Lurk, Tabea
Publisher: name: Technische Hochschule Köln
Year: 2022
Subjects: Datenmanagement
Kunst
FAIR-Principles
Werktypologien
Klassifikationen

description: Die Arbeit untersucht das Datenmanagement (DM) an Kunsthochschulen. Während im ersten Teil anhand von Fallbeispielen eruiert wird, was die Betrachtung von Kunst als Daten aus einer DM-Perspektive bedeuten kann, können durch einen vergleichenden Blick auf etablierte DM-Zyklen und -Modelle anschließend Eckpunkte für das DM in den Künsten festgehalten werden. Zuletzt tragen die FAIR Principles dazu bei, Lücken in der metadatentechnischen Abdeckung der künstlerisch-gestalterischen Werkformen zu erkennen: Dass dieses Mismatching, das insb. mit Blick auf die in DM-Systemen häufig hinterlegten (Werk-)Typologien und Klassifikationen untersucht wird, mitunter beachtliche Effekte haben kann, wird im letzten Teilkapitel der Arbeit und im Fazit verdeutlicht.
Integration of Policy and Reputation based Trust Mechanisms in e-Commerce Industry
Creators: name: Siddiqui, Muhammad Yasir
name: Gir, Alam
Publisher: name: arXiv
Year: 2024
Subjects: subject: Artificial Intelligence (cs.AI)
subject: Computers and Society (cs.CY)
subject: Multimedia (cs.MM)
subject: Social and Information Networks (cs.SI)
subject: FOS: Computer and information sciences

description: The e-commerce systems are being tackled from commerce behavior and internet technologies. Therefore, trust aspect between buyer-seller transactions is a potential element which needs to be addressed in competitive e-commerce industry. The e-commerce industry is currently handling two different trust approaches. First approach consists on centralized mechanism where digital credentials/set of rules assembled, called Policy based trust mechanisms . Second approach consists on decentralized trust mechanisms where reputation, points assembled and shared, called Reputation based trust mechanisms. The difference between reputation and policy based trust mechanism will be analyzed and recommendations would be proposed to increase trust between buyer and seller in e-commerce industry. The integration of trust mechanism is proposed through mapping process, strength of one mechanism with the weakness of other. The proposed model for integrated mechanism will be presented and illustrated how the proposed model will be used in real world e-commerce industry.
Single Cell Gene Expression analysis in a 3D microtissue liver model reveals cell type-specific responses to pro-fibrotic TGF-β1 stimulation
Creators: name: Messner, Catherine
name: Babrak, Lmar
name: Titolo, Gaia
name: Caj, Michaela
name: Miho, Enkelejda
name: Suter-Dick, Laura
Publisher: name: MDPI
Year: 2021
Subjects: Single cell sequencing
In vitro
Liver
Liver fibrosis
Liver microtissues

description: 3D cell culture systems are widely used to study disease mechanisms and therapeutic interventions. Multicellular liver microtissues (MTs) comprising HepaRG, hTERT-HSC and THP-1 maintain multicellular interactions and physiological properties required to mimic liver fibrosis. However, the inherent complexity of multicellular 3D-systems often hinders the discrimination of cell type specific responses. Here, we aimed at applying single cell sequencing (scRNA-seq) to discern the molecular responses of cells involved in the development of fibrosis elicited by TGF-β1. To obtain single cell suspensions from the MTs, an enzymatic dissociation method was optimized. Isolated cells showed good viability, could be re-plated and cultured in 2D, and expressed specific markers determined by scRNA-seq, qRT-PCR, ELISA and immunostaining. The three cell populations were successfully clustered using supervised and unsupervised methods based on scRNA-seq data. TGF-β1 led to a fibrotic phenotype in the MTs, detected as decreased albumin and increased αSMA expression. Cell-type specific responses to the treatment were identified for each of the three cell types. They included HepaRG damage characterized by a decrease in cellular metabolism, prototypical inflammatory responses in THP-1s and extracellular matrix remodeling in hTERT-HSCs. Furthermore, we identified novel cell-specific putative fibrosis markers in hTERT-HSC (COL15A1), and THP-1 (ALOX5AP and LAPTM5).
Time and band limiting for exceptional polynomials
Creators: name: Castro, M. M.
name: Grünbaum, F. A.
name: Zurrián, I.
Publisher: name: arXiv
Year: 2024
Subjects: subject: Spectral Theory (math.SP)
subject: Functional Analysis (math.FA)
subject: Operator Algebras (math.OA)
subject: FOS: Mathematics
subject: 33C45, 22E45, 33C47

description: The "time-and-band limiting" commutative property was found and exploited by D. Slepian, H. Landau and H. Pollak at Bell Labs in the 1960's, and independently by M. Mehta and later by C. Tracy and H. Widom in Random matrix theory. The property in question is the existence of local operators with simple spectrum that commute with naturally appearing global ones.
 Here we give a general result that insures the existence of a commuting differential operator for a given family of exceptional orthogonal polynomials satisfying the "bispectral property". As a main tool we go beyond bispectrality and make use of the notion of Fourier Algebras associated to the given sequence of exceptional polynomials.
 We illustrate this result with two examples, of Hermite and Laguerre type, exhibiting also a nice Perline's form for the commuting differential operator.
Tätigkeitsbericht der Mediathek 2021
Creators: name: Lurk, Tabea
Publisher: name: Hochschule für Gestaltung und Kunst FHNW
Year: 2022
Subjects: Jahresbericht
Mediathek
Sammlungen
Datenmanagement

description: 2021 war in der Mediathek sowohl ein Jahr der Veränderungen und Aktualisierung als auch der Normalisierung und Verstetigung. So fanden sich Nutzende und Mitarbeitende ein weiteres Jahr mit dem ‘neuen Normalzustand’ der pandemiebedingten Änderungen ab und auch für die struktu-rellen Veränderungen wurden konstruktive Lösungen gefunden.
Additional file 1 of ZDHHC5-mediated S-palmitoylation of FAK promotes its membrane localization and epithelial-mesenchymal transition in glioma
Creators: name: Wang, Yang
name: Shen, Na
name: Yang, Yang
name: Xia, Yuan
name: Zhang, Wenhao
name: Lu, Yu
name: Wang, Zhicheng
name: Yang, Ze
name: Wang, Zhangjie
Publisher: name: figshare
Year: 2024
Subjects: Biochemistry
Cell Biology
Molecular Biology
Pharmacology
Chemical Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Cancer
Hematology

description: Additional file 1: Supplementary Figure 1. S-palmitoylation maintains membrane localization of FAK. Supplementary Figure 2. ZDHHC5 regulates FAK S-palmitoylation by directly binding to FAK. Supplementary Figure 3. ZDHHC5 maintains FAK membrane localization and activation via S-palmitoylation. Supplementary Figure 4. FAK is palmitoylated at Cys456. Supplementary Figure 5. ZDHHC5-mediated FAK S-palmitoylation promotes cell proliferation, cell invasion and EMT in vitro. Supplementary Figure 6. ZDHHC5-mediated FAK S-palmitoylation promotes cell proliferation, cell invasion and EMT in vitro.
Hidden in Plain Sight: Scriptless Microarchitectural Attacks via TrueType Font Hinting
Creators: name: Trampert, Leon
name: Schwarz, Michael
Publisher: name: CISPA
Year: 2025

description: Microarchitectural attacks threaten system security and privacy, especially if they can be mounted without native code execution. Recent research has shown that such attacks are possible from within web browsers via JavaScript and WebAssembly. Moreover, recent works have demonstrated that "scriptless" attacks, using only CSS, can be leveraged for side-channel attacks, including cache contention and user fingerprinting.

In this paper, we introduce a new class of scriptless attacks that use the hinting instructions embedded within TrueType font files. We show that the hinting language is sufficiently robust to craft cache attacks, demonstrating cache-contention attacks and precise L1 Prime+Probe attacks. We demonstrate a website fingerprinting attack, as well as a method to track which page of a PDF is currently displayed. Our results demonstrate the practicality of font-based scriptless attacks in real-world scenarios. This emphasizes the need for future mitigations that go beyond traditional scripting languages.
Additional file 2 of ZDHHC5-mediated S-palmitoylation of FAK promotes its membrane localization and epithelial-mesenchymal transition in glioma
Creators: name: Wang, Yang
name: Shen, Na
name: Yang, Yang
name: Xia, Yuan
name: Zhang, Wenhao
name: Lu, Yu
name: Wang, Zhicheng
name: Yang, Ze
name: Wang, Zhangjie
Publisher: name: figshare
Year: 2024
Subjects: Biochemistry
Cell Biology
Molecular Biology
Pharmacology
Chemical Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Cancer
Hematology

description: Additional file 2.
title: Nicht-kultische Triptycha
Creators: name: Pawlik, Miriam
Year: 2008

description: Diese wissenschaftliche Arbeit beschäftigt sich mit dem Aufkommen von Triptycha, die nicht als Altarbilder gedient haben. Es handelt sich um Werke, die die Form eines Flügelaltars besitzen, ihr Bildinhalt weist jedoch aus, dass sie nicht für die Liturgie, die Andacht oder die Religionsausübung im Sinne einer Kulthandlung gestaltet wurden. Acht Triptycha werden im Rahmen der Arbeit untersucht, sie sind zwischen 1500 und 1545 entstanden. Diese bilden ein Phänomen der niederländischen Malerei der frühen Neuzeit, das von der kunstgeschichtlichen Forschung bisher wenig beachtet worden ist. Zielsetzung der Arbeit ist es, die sogenannten „nicht-kultischen Triptycha“ in diesem Zusammenhang zu untersuchen, um dadurch die Basis für eine weitere Forschungsarbeit zu ermöglichen. Ausgehend von einigen Werken des Meisters Hieronymus Bosch ist die Frage von der Forschung gestellt worden, ob es sich um Altarbilder handeln kann. Ausschlaggebend für diese Überlegung ist der Bildinhalt. Der sogenannte „Garten der Lüste“ ist in dieser Hinsicht das umstrittenste Werk. Bis heute herrscht kein allgemeiner Konsens über den Bildinhalt und die Funktion. Seine Ikonographie ist einzigartig. Die Mehrheit der Autoren, die sich mit dem Bild befassten, geht davon aus, dass dieses Triptychon nicht sakral gebraucht worden ist. Jedoch ist immer wieder, auch in der jüngeren Forschung postuliert worden, dass dieses Werk selbstverständlich oder sozusagen selbst erklärend als Altarbild aufzufassen sei. Vertreter dieses Standpunkts stützen ihr Argument auf die Art des Bildträgers, der in der traditionellen Form eines Flügelaltars gestaltet ist. Es ist diesen unvorstellbar, dass ein Werk, das in diesem Medium geschaffen wurde, nicht für die Religionsausübung bestimmt gewesen sein soll. Die Untersuchung der „nicht-kultischen Triptycha“ macht deutlich, dass für diese Bilder eine Altarbild-Funktion auszuschließen ist. Die Bildinhalte und die Ikonographien werden im Hinblick auf ihren innovativen Wert und eine künstlerische Schaffensfreiheit betrachtet. In diesem Zusammenhang werden Spekulationen über eine mögliche Bestimmung dieser Werke angestellt. Der Katalog erschließt den kunstgeschichtlichen Forschungstand der „nicht-kultischenTriptycha“.
Ambivalenzen überwinden. Editorial
Creators: name: Beywl, Wolfgang
name: Künzli, Christine
Publisher: name: ZIEL
Year: 2020
Subjects: subject: Weiterbildung
subject: Fortbildung
subject: Wissenschaftliche Weiterbildung
subject: Bildung
subject: Lernen
subject: 370 - Erziehung, Schul- und Bildungswesen

description: Das Heft behandelt Weiterbildungen, die von erziehungswissenschaftlich Forschenden konzipiert und geleitet werden, und an denen pädagogische Expertinnen und Experten aus der Bildungspraxis teilnehmen.
Dynamics of a Model of Polluted Lakes via Fractal-Fractional Operators with Two Different Numerical Algorithms
Creators: name: Kanwal, Tanzeela
name: Hussain, Azhar
name: Avcı, İbrahim
name: Etemad, Sina
name: Rezapour, Shahram
name: Torres, Delfim F. M.
Publisher: name: arXiv
Year: 2024
Subjects: subject: Dynamical Systems (math.DS)
subject: FOS: Mathematics
subject: 34A08, 65P99

description: We employ Mittag-Leffler type kernels to solve a system of fractional differential equations using fractal-fractional (FF) operators with two fractal and fractional orders. Using the notion of FF-derivatives with nonsingular and nonlocal fading memory, a model of three polluted lakes with one source of pollution is investigated. The properties of a non-decreasing and compact mapping are used in order to prove the existence of a solution for the FF-model of polluted lake system. For this purpose, the Leray-Schauder theorem is used. After exploring stability requirements in four versions, the proposed model of polluted lakes system is then simulated using two new numerical techniques based on Adams-Bashforth and Newton polynomials methods. The effect of fractal-fractional differentiation is illustrated numerically. Moreover, the effect of the FF-derivatives is shown under three specific input models of the pollutant: linear, exponentially decaying, and periodic.
description: This is a preprint of a paper whose final and definite form is published Open Access in 'Chaos Solitons Fractals' at [https://doi.org/10.1016/j.chaos.2024.114653]
Additional file 1 of Tumor-derived small extracellular vesicles facilitate omental metastasis of ovarian cancer by triggering activation of mesenchymal stem cells
Creators: name: Gong, Lanqing
name: Li, Guoqing
name: Yi, Xiaoqing
name: Han, Qing
name: Wu, Qiulei
name: Ying, Feiquan
name: Shen, Lu
name: Cao, Ying
name: Liu, Xiaoli
name: Gao, Lingling
name: Li, Wenhan
name: Wang, Zehua
name: Cai, Jing
Publisher: name: figshare
Year: 2024
Subjects: subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Chemical Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Cancer

description: Additional file 1.
Lehren als Profession. Kriterien guten Unterrichts - eine Synopse
Creators: name: Beywl, Wolfgang
name: Schmid, Philipp
Publisher: name: ZIEL
Year: 2014
Subjects: subject: Kriterien guten Unterrichts
subject: Lehren und Lernen sichtbar machen
subject: Feedback
subject: Weiterbildung
subject: Lehren als Profession
subject: Guter Unterricht
subject: Synoptische Auswertung
subject: 370 - Erziehung, Schul- und Bildungswesen

description: Das Wissen über guten, bildenden, lernförderlichen Unterricht ist in den vergangenen Jahrzehnten stark angewachsen und auch leichter zugänglich geworden. Es gibt weiterhin teils emotional verhärtete Debatten, bis hinein in die Tagespresse, was denn guten Unterricht ausmacht. Es scheint grundsätzlich schwer zu sein, konsensfähige Antworten auf die Frage nach den Kriterien guten Unterrichts zu geben. Die nachfolgenden Überlegungen basieren auf einer synoptischen Auswertung der Forschungsergebnisse dreier breit rezipierter Autoren.
The Rewrite Collaborative Framework Browser Extension
Creators: name: Bruder, Johannes
name: Sobecka, Karolina
name: Granzotto, Alberto
name: Frei, Fabian
name: Suess, Solveig
name: Kolb, Lucie
Publisher: name: Fachhochschule Nordwestschweiz FHNW
Year: 2022
Subjects: Annotation
Textarbeit

description: A browser extension, based on the open access framework hypothes.is, which allows for collective reading and annotation of complex texts (e.g. policy texts, legal texts, international treaties). The extension can be used for teaching & research, consensus-building and negotiation, reviewing or rewriting. Please consult the manuals before installing and using the extension.
Kooperation von Logopädinnen und Logopäden in Forschung und klinischer Praxis im Aphasieprojekt «E-Inclusion»
Creators: name: Kuntner, Katrin Petra
name: Widmer Beierlein, Sandra
name: Elsener, Claudia
name: Dittmann-Aubert, Fanny
name: Hauser, Norina
name: Moriz, Matthias
name: Wegele, Maria
name: Winkler, Manon
name: Harvey, Morgaine
name: Falcón García, Noelia
name: Altermatt, Sven
name: Müller, Christine
name: Degen, Markus
name: Reymond, Claire
name: Hemm-Ode, Simone
name: Blechschmidt, Anja
Publisher: name: Schweizerische Arbeitsgemeinschaft für Logopädie
Year: 2022

description: Im Zuge der evidenzbasierten Praxis sind im Bereich der Logopädie/Sprachtherapie neben der Praxisexper - tise wissenschaftliche Erkenntnisse in die Therapie zu integrieren. Dafür wird mehr fachspezifische Forschung benötigt, die auf Unterstützung der Praxis angewiesen ist. Das Ziel des Artikels ist es, die für Logopädie-For - schung notwendige intradisziplinäre Kooperation von logopädischen Forscherinnen und Forschern sowie Prak- tikerinnen und Praktikern anhand des interdisziplinären Aphasieprojektes «E-Inclusion» der Fachhochschule Nordwestschweiz (FHNW) zu beleuchten. Dazu wird das Aphasieprojekt vorgestellt, welches vier Ziele verfolgte: (1) Auswirkungen von Dialekt und Hochdeutsch sowie (2) von Fotografien und Illustrationen auf die Bildbenenn- leistung bei u. a. Personen mit Aphasie zu untersuchen, (3) mithilfe akustisch-objektiver Sprachsignalanalysen Leistungsveränderungen der Aphasie zu ermitteln und (4) aus diesen Erkenntnissen eine Prototypen-Appli- kation zu entwickeln. Nach der Projektvorstellung gewähren logopädische Praktikerinnen und Praktiker aus Partnerinstitutionen sowie das logopädische Forschungsteam Einblick in die intradisziplinäre Kooperation. Ins- besondere die Rekrutierung der Studienteilnehmenden erforderte eine enge Zusammenarbeit. Als Resultat des Artikels zeigt sich, dass die Kooperation auch unter erschwerten Bedingungen wie zunehmender Zeitdruck im klinisch-therapeutischen Setting für beide Seiten erfolgreich, bedeutsam und bereichernd war. Daraus abgelei- tete Erkenntnisse für kooperative Projekte werden im Fazit dieses Artikels formuliert. Zukünftig können so Logo- pädinnen und Logopäden aus Forschung und Praxis, wie auch Betroffene, von der inter- und intradisziplinären Kooperation im Bereich der Logopädie bzw. in Folgeprojekten des «E-Inclusion»-Forschungsteams profitieren.
Spectroscopic Studies of Metal Halide Perovskite Materials
Creators: name: ruan, shuai
Publisher: name: Monash University
Year: 2020
Subjects: classificationCode: 401602
subject: Composite and hybrid materials

description: This thesis describes spectroscopic studies of mixed-halide and mixed-cation perovskites aimed at elucidating the processes of phase transition, phase segregation and material decomposition upon exterior stimuli such as light, heat or oxygen. Raman spectroscopy and in-situ photoluminescence spectroscopy are employed as key experimental techniques, with single crystals in particular studied to overcome limitations of studying polycrystalline thin films. These studies provide new insight into fundamental understanding Hybrid Organic-Inorganic perovskites (HOIPs) which will be of benefit for exploiting their performance in solar cells and beyond.
"The first one to pick up the phone": Forms of recruitment for low-skilled jobs
Creators: name: Nadai, Eva
name: Hübscher, Robin
Publisher: name: Seismo
Year: 2022
Subjects: subject: recruitment
subject: low-skilled jobs
subject: labour market intermediaries
subject: sociology of conventions
subject: valorization
subject: 300 - Sozialwissenschaften, Soziologie, Anthropologie

description: Based on a qualitative study on the employability of workers without vocational qualification in five different industries, this article examines hiring practices in the labour market segment of low-skilled jobs from a sociology of convention perspective. In the absence of educational signals, employers use personal networks and trial workdays to reduce the uncertainty regarding the quality of job applicants. However, professional and technical intermediaries become more important, thus leading to the formalization of recruitment channels and valorisation.
Additional file 2 of Tumor-derived small extracellular vesicles facilitate omental metastasis of ovarian cancer by triggering activation of mesenchymal stem cells
Creators: name: Gong, Lanqing
name: Li, Guoqing
name: Yi, Xiaoqing
name: Han, Qing
name: Wu, Qiulei
name: Ying, Feiquan
name: Shen, Lu
name: Cao, Ying
name: Liu, Xiaoli
name: Gao, Lingling
name: Li, Wenhan
name: Wang, Zehua
name: Cai, Jing
Publisher: name: figshare
Year: 2024
Subjects: subject: Biochemistry
subject: Medicine
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Chemical Sciences not elsewhere classified
subject: Biological Sciences not elsewhere classified
subject: Developmental Biology
subject: Cancer

description: Additional file 2.
title: Einfluss von filmischen Geschichtsdarstellungen auf das historische Bewusstsein von Jugendlichen
Creators: name: Kienberger, Martina
Year: 2008

description: Diese Arbeit befasst sich mit den Möglichkeiten der Einflussnahme filmischer Geschichtsdarstellungen auf das historische Bewusstsein von Jugendlichen, mittels sowohl theoretischer als auch praktisch-empirischer Ansätze. Im theoretischen Teil der Arbeit wird ein Überblick über die relevante Fachliteratur in den Bereichen Geschichtsbewusstsein, Charakteristika filmischer Geschichtsdarstellungen, Bedingungen der filmischen Rezeption und Einflussnahme von Film sowie über die Theorien zu möglichen Beeinflussungen des historischen Bewusstseins Jugendlicher durch Film gegeben. Weiters werden die Möglichkeiten zur Erforschung des Geschichtsbewusstseins und des filmischen Einflusses dargestellt und unter Berücksichtigung des Forschungsstandes und des Bedarfs an bestimmten empirischen Daten ein neues Konzept zur Untersuchung des Forschungsgegenstandes entwickelt. Der zweite Teil der Arbeit befasst sich mit der praktischen Umsetzung dieses Konzepts in einer Studie unter ca. 160 Schülern und deren Lehrern, präsentiert werden Forschungsdesign, Untersuchungsmethode, erste Ergebnisse und Konsequenzen für die weitere Forschung.