Additional file 1 of Brahma-related gene 1 acts as a profibrotic mediator and targeting it by micheliolide ameliorates peritoneal fibrosis
Creators: name: Li, Shuting
name: Luo, Congwei
name: Chen, Sijia
name: Zhuang, Yiyi
name: Ji, Yue
name: Zeng, Yiqun
name: Zeng, Yao
name: He, Xiaoyang
name: Xiao, Jing
name: Wang, Huizhen
name: Chen, Xiaowen
name: Long, Haibo
name: Peng, Fenfen
Publisher: name: figshare
Year: 2024
Subjects: subject: Biophysics
subject: Biochemistry
subject: Cell Biology
subject: Genetics
subject: FOS: Biological sciences
subject: Molecular Biology
subject: Pharmacology
subject: Chemical Sciences not elsewhere classified
subject: Immunology
subject: FOS: Clinical medicine
subject: Biological Sciences not elsewhere classified
subject: Marine Biology
subject: Cancer

description: Additional file 1: Figure S1. BRG1 is increased in a mouse model of PD and in peritoneal mesothelial cells with TGF-β1 or PD fluid stimulation. a Quantitative data of Western blot analyses show the visceral peritoneum expression of BRG1, Fibronectin, a-SMA. *P&lt;0.05 versus saline mice.n=6 per group. b–d Quantitative data of Western blot analyses show the expression of BRG1 (b), Fibronectin (c), a-SMA (d) in HMrSV5 cells challenged by TGF-β1 for 0, 12, 24 and 48 hours, respectively. *P&lt;0.05 versus time zero. e–h Quantitative data of Western blot analyses show the expression of BRG1 (e), Collagen I (f), E-cadherin (g) and TGF-β1 (h) in HMrSV5 cells stimulated by PDF. *P&lt;0.05 versus saline. i Quantitative data of Western blot analyses show the expression of BRG1, Collagen I, E-cadherin, Vimentin,and Fibronectin in RPMCs stimulated by TGF-β1. *P&lt;0.05 versus control group. j Quantitative data of Western blot analyses show the expression of BRG1, Collagen I, E-cadherin, TGF-β1, and a-SMA in RPMCs stimulated by PDF. *P&lt;0.05 versus saline. Figure S2. BRG1 promotes fibrotic responses in vitro. a Graphic presentation shows the mRNA expression of BRG1 in HMrSV5 cells in different groups as indicated. The mRNA level of BRG1 was detected by Quantitative real-time PCR. *P&lt;0.05 versus Vector. b Graphic presentation shows the mRNA expression of BRG1 in RPMCs in different groups as indicated.*P&lt;0.05 versus Vector. c Quantitative data of Western blot analyses show the expression of BRG1, E-cadherin,TGF-β1. Triangle represents incremental plasmid dosage (2ug,3ug and 4ug/plate). *P&lt;0.05 versus Vector. d Graphic presentation shows the mRNA expression of BRG1 in different groups as indicated in RPMCs.*P&lt;0.05 versus Vector. e Quantitative data of Western blot analyses show the expression of BRG1, Vimentin, a-SMA, Collagen I in different groups are presented. *P&lt;0.05 versus siNC, #P&lt;0.05 versus siNC in the presence of TGF-β1. Figure S3. BRG1 inhibition has no effect on phosphorylation of ERK1/2, P38 MAPK and JNK in vitro. a, b Western blot analyses show that BRG1 inhibition by siRNA had no effect on phosphorylation of ERK1/2. Representative Western blot (a) and quantitative data (b) in different groups are presented. c, d Western blot analyses show that BRG1 inhibition had no effect on P38 phosphorylation. Representative Western blot (c) and quantitative data (d) in different groups are presented. e, f Western blot analyses show that BRG1 inhibition had no effect on phosphorylation of JNK. Representative Western blot (e) and quantitative data (f) in different groups are presented. Figure S4. The role of MCL on BRG1-induced fibrotic responses. a Schematic representation of BRG1 mutants. BRG1 was divided into three regions: a ATPase domain,a N-terminal region, and a C-terminal region. Each mutant expression plasmids contains a C-terminal flag epitope tag. (b)Western blot analyses show that expression of Flag after BRG1 full length or BRG1 N1540A mutant transfection. c–g Quantitative data of Western blot analyses show the expression of Collagen I (c), Fibronectin (d), P-Smad3 (e), TGF-β1 (f) and a-SMA (g) in different groups are presented. *P&lt;0.05 versus Vector.#P&lt;0.05 versus pBRG1. Table S1. The detail information of antibodies used in this study. Table S2. The sequence of siRNA. Table S3. RT-qPCR primer sequences.