title: Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð¸ Ð¾Ð¿ÑÐ¸Ð¼Ð¸Ð·Ð°ÑÐ¸Ñ Ð±Ð¸Ð¾-Ð¸Ð¼Ð¿ÐµÐ´Ð°Ð½ÑÐ½ÑÑ ÑÐ¸ÑÐ¾ÑÐµÐ½ÑÐ¾ÑÐ¾Ð²
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Development and optimization of bio-impedance cytosensors
Creators: name: ÐÐ±ÐµÐ»Ð¸Ñ, ÐÐ½Ð½Ð°
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: ÑÐ»ÐµÐºÑÑÐ¸ÑÐµÑÐºÐ°Ñ Ð¸Ð¼Ð¿ÐµÐ´Ð°Ð½ÑÐ½Ð°Ñ ÑÐ¿ÐµÐºÑÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ
Ð¼ÑÐ»ÑÑÐ¸ÑÐ»ÐµÐºÑÑÐ¾Ð´Ð½Ð°Ñ Ð¼Ð°ÑÑÐ¸ÑÐ°
Ð°Ð´Ð³ÐµÐ·Ð¸Ñ ÑÑÐºÐ°ÑÐ¸Ð¾ÑÐ¸ÑÐµÑÐºÐ¸Ñ ÐºÐ»ÐµÑÐ¾Ðº
ÑÐ¾ÐºÐ°Ð»ÑÐ½ÑÐµ ÐºÐ¾Ð½ÑÐ°ÐºÑÑ
electrical impedance spectroscopy
multielectrode array
adhesion of eukaryotic cells
focal contacts

description: Ð¡ÐµÐ³Ð¾Ð´Ð½Ñ ÑÐ»ÐµÐºÑÑÐ¸ÑÐµÑÐºÐ°Ñ Ð¸Ð¼Ð¿ÐµÐ´Ð°Ð½ÑÐ½Ð°Ñ ÑÐ¿ÐµÐºÑÑÐ¾ÑÐºÐ¾Ð¿Ð¸Ñ (Ð­ÐÐ¡) Ð¸ ÑÐµÑÐ½Ð¾-Ð»Ð¾Ð³Ð¸Ñ Ð¼ÑÐ»ÑÑÐ¸ÑÐ»ÐµÐºÑÑÐ¾Ð´Ð½ÑÑ Ð¼Ð°ÑÑÐ¸Ñ (ÐÐ­Ð) ÑÐ¸ÑÐ¾ÐºÐ¾ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·ÑÑÑÑÑ Ð´Ð»Ñ ÑÐµ-ÑÐµÐ½Ð¸Ñ Ð·Ð°Ð´Ð°Ñ ÑÐ¸ÑÐ¾ÑÐµÐ½ÑÐ¾ÑÐ¸ÐºÐ¸, Ð½Ð°Ð¿ÑÐ¸Ð¼ÐµÑ Ð´Ð»Ñ Ð¾ÑÐµÐ½ÐºÐ¸ Ð¶Ð¸Ð·Ð½ÐµÑÐ¿Ð¾ÑÐ¾Ð±Ð½Ð¾ÑÑÐ¸ÐºÐ»ÐµÑÐ¾Ðºinvitro, Ð´Ð»Ñ Ð¸Ð·ÑÑÐµÐ½Ð¸Ñ Ð²Ð¾Ð·Ð´ÐµÐ¹ÑÐ²Ð¸Ñ ÑÐ°Ð·Ð»Ð¸ÑÐ½ÑÑ ÑÐ°ÑÐ¼Ð°ÐºÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸ÑÐ¿ÑÐµÐ¿Ð°ÑÐ°ÑÐ¾Ð² Ð½Ð° ÑÐºÐ¾ÑÐ¾ÑÑÑ Ð·Ð°Ð¶Ð¸Ð²Ð»ÐµÐ½Ð¸Ñ ÑÐ°Ð½ Ð¸ Ñ.Ð¿. Ð ÑÐ¾ Ð¶Ðµ Ð²ÑÐµÐ¼Ñ ÑÐ¾Ð²ÑÐµ-Ð¼ÐµÐ½Ð½ÑÐµ ÐºÐ¾Ð¼Ð¼ÐµÑÑÐµÑÐºÐ¸ Ð´Ð¾ÑÑÑÐ¿Ð½ÑÐµ Ð¸Ð¼Ð¿ÐµÐ´Ð°Ð½ÑÐ½ÑÐµ ÑÐ¸ÑÐ¾ÑÐµÐ½ÑÐ¾ÑÑ (ECIS Ð¾ÑApplied BioPhysics Ð¸ xCelligence Ð¾Ñ ACEA Biosciences) Ð¾Ð±Ð»Ð°Ð´Ð°ÑÑ ÑÑÐ´Ð¾Ð¼Ð½ÐµÐ´Ð¾ÑÑÐ°ÑÐºÐ¾Ð²: Ð½Ð¸Ð·ÐºÐ¸Ð¼ Ð²ÑÐµÐ¼ÐµÐ½Ð½ÑÐ¼ Ð¸ ÑÐ°ÑÑÐ¾ÑÐ½ÑÐ¼ ÑÐ°Ð·ÑÐµÑÐµÐ½Ð¸ÐµÐ¼, Ð° ÑÐ°ÐºÐ¶ÐµÐ½ÐµÐ²Ð¾Ð·Ð¼Ð¾Ð¶Ð½Ð¾ÑÑÑÑ ÑÐ°Ð±Ð¾ÑÑ Ñ ÑÑÑÐ¿ÐµÐ½Ð·Ð¸Ð¾Ð½Ð½ÑÐ¼Ð¸ ÐºÑÐ»ÑÑÑÑÐ°Ð¼Ð¸ Ð¸ ÐºÑÐ»ÑÑÑÑÐ°Ð¼Ð¸ÑÐ¾ ÑÐ»Ð°Ð±Ð¾Ð¹ Ð°Ð´Ð³ÐµÐ·Ð¸ÐµÐ¹. Ð¦ÐµÐ»ÑÑ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ ÑÐ²Ð»ÑÐ»Ð°ÑÑ ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐºÐ° Ð¸ Ð°Ð¿ÑÐ¾-Ð±Ð°ÑÐ¸Ñ ÑÐ¸ÑÐ¾ÑÐµÐ½ÑÐ¾ÑÐ° Ð´Ð»Ñ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ ÑÑÑÐ¿ÐµÐ½Ð·Ð¸Ð¾Ð½Ð½ÑÑ ÐºÐ»ÐµÑÐ¾Ðº Ñ Ð¿Ð¾Ð¼Ð¾-ÑÑÑ Ð²ÑÑÐ¾ÐºÐ¾ÑÐºÐ¾ÑÐ¾ÑÑÐ½Ð¾Ð¹ Ð¸ Ð²ÑÑÐ¾ÐºÐ¾ÑÐ°Ð·ÑÐµÑÐ°ÑÑÐµÐ¹ Ð­ÐÐ¡. ÐÐ»Ñ Ð´Ð¾ÑÑÐ¸Ð¶ÐµÐ½Ð¸ÑÐ´Ð°Ð½Ð½Ð¾Ð¹ ÑÐµÐ»Ð¸ Ð±ÑÐ»Ð° ÑÐ°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð° ÑÐµÑÐ½Ð¾Ð»Ð¾Ð³Ð¸Ñ Ð¿ÑÐ¾Ð±Ð¾Ð¿Ð¾Ð´Ð³Ð¾ÑÐ¾Ð²ÐºÐ¸ Ð¿Ð¾Ð²ÐµÑÑÐ½Ð¾-ÑÑÐ¸ ÐÐ­Ð, Ð¿Ð¾Ð·Ð²Ð¾Ð»ÑÑÑÐ°Ñ ÑÐ»ÑÑÑÐ¸ÑÑ Ð°Ð´Ð³ÐµÐ·Ð¸Ñ ÐºÐ»ÐµÑÐ¾Ðº Ð½Ð° ÑÐ»ÐµÐºÑÑÐ¾Ð´Ð°Ñ Ð±ÐµÐ·ÑÑÐµÑÐ±Ð° Ð´Ð»Ñ Ð¸Ñ ÑÐ»ÐµÐºÑÑÐ¸ÑÐµÑÐºÐ¸Ñ ÑÐ²Ð¾Ð¹ÑÑÐ². Ð Ð°Ð·ÑÐ°Ð±Ð¾ÑÐ°Ð½Ð½Ð°Ñ ÑÐµÑÐ½Ð¸ÐºÐ° Ð±ÑÐ»Ð° Ð¸Ñ-Ð¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð° Ð´Ð»Ñ ÑÐ¾Ð·Ð´Ð°Ð½Ð¸Ñ Ð±Ð¸Ð¾-ÑÐ»ÐµÐºÑÑÐ¾Ð½Ð½Ð¾Ð³Ð¾ Ð¸Ð¼Ð¿ÐµÐ´Ð°Ð½ÑÐ½Ð¾Ð³Ð¾ ÑÐ¸ÑÐ¾ÑÐµÐ½ÑÐ¾ÑÐ°,ÑÑÐ²ÑÑÐ²Ð¸ÑÐµÐ»ÑÐ½ÑÐ¼ ÑÐ»ÐµÐ¼ÐµÐ½ÑÐ¾Ð¼ ÐºÐ¾ÑÐ¾ÑÐ¾Ð³Ð¾ ÑÐ²Ð»ÑÑÑÑÑ ÑÑÑÐ¿ÐµÐ½Ð·Ð¸Ð¾Ð½Ð½ÑÐµ ÐºÐ»ÐµÑÐºÐ¸.ÐÐ°Ð½Ð½ÑÐ¹ ÑÐ¸ÑÐ¾ÑÐµÐ½ÑÐ¾Ñ Ð±ÑÐ» Ð°Ð¿ÑÐ¾Ð±Ð¸ÑÐ¾Ð²Ð°Ð½ Ð½Ð° Ð¿ÑÐ¸Ð¼ÐµÑÐµ ÑÐµÐ³Ð¸ÑÑÑÐ°ÑÐ¸Ð¸ Ð³Ð¸Ð±ÐµÐ»Ð¸ÐºÐ»ÐµÑÐ¾Ðº Ð¿Ð¾Ð´ Ð´ÐµÐ¹ÑÑÐ²Ð¸ÐµÐ¼ Ð¿Ð¾ÑÐ°Ð¶Ð°ÑÑÐµÐ³Ð¾ Ð£Ð¤ Ð¸Ð·Ð»ÑÑÐµÐ½Ð¸Ñ.
description: Nowadays, the combination of the electrical impedance spectroscopy(EIS) and multielectrode arraysâ (MEA) technology is widely used in cy-tosensorics,e.g.for living cells viability estimationin vitro, for studyingwound healing processesetc. Despite the great success achieved in cytol-ogy by using commercially available impedance-based cytosensors (ECISby Applied BioPhysics and xCelligence by ACEA Biosciences), these de-vices have multiple limitations: low frequency- and time-resolution, andalso impossibility to studying suspension cells and cells with poor adhe-sion. In the same time removal of these disadvantages by means progressiveimpedance-measurement techniques and special electrode surface modifica-tion will significantly increase the area of cytosensors application. In thisstudy, we present modification of the electrical cell-substrate impedancesensing technology which make it applicable for suspension cells and cellswith poor adhesion. The key idea of the proposed technique consist in coat-ing electrodes with poly-DL-lysine. We have show that this procedure notonly provide negligible influence on the electrode impedance, but also al-lows to study viability of the suspensions cells K562 in real time.