Additional file 1 of Exosome-transmitted circCABIN1 promotes temozolomide resistance in glioblastoma via sustaining ErbB downstream signaling
Creators: name: Liu, Xiao
name: Guo, Qingdong
name: Gao, Guangxun
name: Cao, Zhengcong
name: Guan, Zhihao
name: Jia, Bo
name: Wang, Weizhong
name: Zhang, Kuo
name: Zhang, Wangqian
name: Wang, Shuning
name: Li, Weina
name: Hao, Qiang
name: Zhang, Yingqi
name: Li, Meng
name: Zhang, Wei
name: Gu, Jintao
Publisher: name: figshare
Year: 2024
Subjects: subject: Biophysics
subject: Medicine
subject: Cell Biology
subject: Molecular Biology
subject: Neuroscience
subject: Pharmacology
subject: Biotechnology
subject: Ecology
subject: FOS: Biological sciences
subject: Information Systems not elsewhere classified
subject: Developmental Biology
subject: Cancer
subject: Mental Health
subject: Hematology
subject: Infectious Diseases
subject: FOS: Health sciences
subject: Virology
subject: Computational Biology

description: Additional file 1. Additional materials. Fig. S1 Related to Fig. 1. A Confirmation of the drug resistance of TMZ-resistant GBM02 cells in vivo. Left, IVIS detects bioluminescence signals. Right, quantification of bioluminescent imaging signal intensities. B Transmission electron micrograph of Res-exo and Sen-exo. C The effect of GW4869 and Rab27a/b siRNA on LN229 / LN229 TMZ resistant cells by CCK-8 assay. D Cells administrated Res-exo or Sen-exo were subjected to FACS to detected apoptosis. E Schematic image and statistical chart of processing of determination time of IC50 after Res-exo or Sen-exo treatment. F The effect of Res-exo or Res-exo plus Sen-exo in LN229 cells by CCK-8 assay. Fig. S2 Related to Fig. 2. A-B Circus plot and heatmap presents the distribution and expression profiles of the detected and differentially expressed circRNAs on human chromosomes. C The existence of circCABIN1 was confirmed by RT-PCR and gel electrophoresis using convergent and divergent primers. D The level of circCABIN1 and linear CABIN1 treated with RNase R was detected by RT-PCR and gel electrophoresis. E Relative level of circCABIN1 and linear CABIN1 treated with RNase R was detected by qRT-PCR. F Relative level of circCABIN1 and linear CABIN1 treated with Actinomycin D was detected by qRT-PCR. G TMZ resistant cells were transfected with circCABIN1KD and apoptosis was detected by caspase3/7 experiment. Scale bar, 10μm. H The expression of CABIN1 in different stages GBM and Kaplan-Meier analysis of OS in the high and low CABIN1 groups in public database. Fig. S3 Related to Fig. 4. A GBM cells administrated with Sen-exo were subjected to FACS to detect the proportion of CD44+CD133+ cells. B The expression of circCABIN1 in the CD44+CD133+ or CD44－CD133－ cells was analyzed by qRT-PCR. C The expression of circCABIN1 in the tumorsphere and adherent from orthotopic GBM xenograft mice was analyzed by qRT-PCR. D Limiting dilution analysis assays detected the stem cell properties of cells transfected with shNC or circCABIN1KD. Fig. S4 Related to Fig. 5. A The expression of miR-637 in the CD44+CD133+ or CD44－CD133－ cells was analyzed by qRT-PCR. B The expression of miR-637 in TMZ resistant and parental cells was analyzed by qRT-PCR. C Colony formation assay detected the effect of cells treated with control vector, miR-637 mimics alone or miR-637 mimics plus circCABIN1OE. Fig. S5 Related to Fig. 6. A Kaplan-Meier analysis of OS in the high and low CITED4, ITGA10, GLDC and EIF4EBP3 groups in TCGA. B Kaplan-Meier analysis of OS in the high and low OLFML3 groups in CGGA, Gravendeel and Rembrandt database. C The expression of OLFML3 in glioma and matched normal tissue in public database. D KEGG pathway analysis demonstrated that regulating pluripotency of stem cells was involved and might be the downstream of OLFML3. E The correlation between OLFML3 and key stemness molecules according to CGGA database. F Colony formation assay detected the effect of cells treated with OLFML3 recombinant protein (0.5μg/mL). G The expression of OLFML3 in TMZ resistant and parental cells were analyzed by western blot. Fig. S6 Related to Fig. 7. A-B Detection of circCABIN1 and OLFML3 knockdown efficiency by multi-siRNA. C The size distribution of ummod-exo and multi-siRNA-exo measured by NANO SIGHT. D Transmission electron micrograph of ummod-exo and multi-siRNA-exo. E The effect of ANG-multi-siNC-exo or ANG-multi-siRNA-exo plus TMZ treatment in cells. Left, apoptosis was detected by caspase3/7 experiment. Scale bar, 10μm. Right, cell viability was detected by CCK-8 assay. F The expression of OLFML3 in cells administrated with ANG-multi-siNC-exo or ANG-multi-siRNA-exo by western blot. G Clinical chemistry and hematology parameters for ANG-multi-siRNA-exo or Saline treated mice. H Body weight of BALB/c mice post-intravenous injection of ANG-multi-siRNA-exo or Saline. I Histological analyses of liver, heart, kidney, lung and spleen sections stained with H&amp;E of BALB/c mice post-intravenous injection of ANG-multi-siRNA-exo or Saline for 12 days (one dose every other day).