cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Guanine nucleotide regulation of agonist binding to muscarinic cholinergic receptors. Relation to efficacy of agonists for stimulation of phosphoinositide breakdown and Ca 2+ mobilization
Creators: name: Masters, S B
name: Evans, T
name: Brown, J H
name: Hepler, J R
name: Harden, T K
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1985

description: The efficacies of a series of six muscarinic cholinergic receptor agonists for stimulation of phosphoinositide breakdown and unidirectional efflux of 45Ca2+ in 1321N1 human astrocytoma cells were compared with the relative capacity of these agonists for formation of a GTP-sensitive high-affinity binding state in washed membranes. Carbachol and methacholine were 'full' agonists as regards phosphoinositide breakdown and Ca2+ mobilization, whereas bethanechol, arecoline and oxotremorine were 'partial' agonists for these two responses. Pilocarpine was the least efficacious of the six drugs tested. Except for pilocarpine, competition curves generated with the agonists and [3H]quinuclidinyl benzilate did not follow the Law of Mass Action for ligand interaction at a single site. Non-linear regression analyses of these data indicated that the data significantly better fit a two-, rather than a single-, site model with a high- and a low-affinity binding component. Competition curves generated in the presence of GTP were shifted to the right, and the extent of receptors in the high-affinity agonist-binding state was decreased. The relative efficacies of the six agonists for stimulation of phosphoinositide breakdown and Ca2+ mobilization were significantly correlated with the difference in affinities (KL/KH) between the two affinity states for each agonist. The relative efficacy of the agonists for stimulation of Ca2+ mobilization also was significantly correlated with the extent of receptors in the high-affinity state (%H) for each agonist. The results suggest that interaction with an as-yet unidentified guanine nucleotide regulatory protein is important in the mechanism whereby muscarinic receptors stimulate phosphoinositide breakdown in 1321N1 astrocytoma cells.
Lie symmetries applied to guaranteed integration: application to mobile robotics localisation
Creators: name: Damers, Julien
name: Jaulin, Luc
name: Rohou, Simon
Publisher: name: Hannover : Gottfried Wilhelm Leibniz Universität
Year: 2022
Subjects: subject: Guaranteed Integration
subject: Lie Symmetries
subject: Localisation
subject: Robotics
subject: Konferenzschrift
subject: Dewey Decimal Classification::500 | Naturwissenschaften::510 | Mathematik

description: [no abstract available]
Efficient engineering analysis with imprecise probabilities
Creators: name: Beer, Michael
name: Broggi, Matteo
name: Faes, Matthias G.R.
name: Valdebenito, Marcos A.
name: Wie, Pengfei
Publisher: name: Hannover : Gottfried Wilhelm Leibniz Universität
Year: 2022
Subjects: subject: Konferenzschrift
subject: Dewey Decimal Classification::500 | Naturwissenschaften::510 | Mathematik

description: [no abstract available]
G1 Dynamics at the Crossroads of Pluripotency and Cancer
Creators: name: Fleifel, Dalia
name: Cook, Jeanette Gowen
Publisher: name: MDPI
Year: 2023

description: G1 cell cycle phase dynamics are regulated by intricate networks involving cyclins, cyclin-dependent kinases (CDKs), and CDK inhibitors, which control G1 progression and ensure proper cell cycle transitions. Moreover, adequate origin licensing in G1 phase, the first committed step of DNA replication in the subsequent S phase, is essential to maintain genome integrity. In this review, we highlight the intriguing parallels and disparities in G1 dynamics between stem cells and cancer cells, focusing on their regulatory mechanisms and functional outcomes. Notably, SOX2, OCT4, KLF4, and the pluripotency reprogramming facilitator c-MYC, known for their role in establishing and maintaining stem cell pluripotency, are also aberrantly expressed in certain cancer cells. In this review, we discuss recent advances in understanding the regulatory role of these pluripotency factors in G1 dynamics in the context of stem cells and cancer cells, which may offer new insights into the interconnections between pluripotency and tumorigenesis.
On Planar Algebraic Curves and Holonomic $\mathcal{D}$-modules in Positive Characteristic
Creators: name: Kanel-Belov, Alexei
name: Elishev, Andrey
Publisher: name: arXiv
Year: 2014
Subjects: subject: Algebraic Geometry (math.AG)
subject: FOS: Mathematics

description: In this paper we study a correspondence between cyclic modules over the first Weyl algebra and planar algebraic curves in positive characteristic. In particular, we show that any such curve has a preimage under a morphism of certain ind-schemes. This property might pave the way for an indirect proof of existence of a canonical isomorphism between the group of algebra automorphisms of the first Weyl algebra over the field complex numbers and the group of polynomial symplectomorphisms of $\mathbb{C}^2$.
description: 14 pages
Mucin biosynthesis. Properties of a bovine tracheal mucin β-6- N -acetylglucosaminyltransferase
Creators: name: Cheng, P W
name: Little, M R
name: Wei, R
name: Wingert, W E
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1985

description: We have characterized a bovine tracheal mucin beta-6-N-acetylglucosaminyltransferase that catalyses the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to the C-6 of the N-acetylgalactosamine residue of galactosyl-beta 1----3-N-acetylgalactosamine. Optimal enzyme activity was obtained between pH 7.5-8.5, at 5mM-MnCl2, and at 0.06-0.08% (v/v) Triton X-100 (or Nonidet P-40), or 0.5-5.0% (v/v) Tween 20. Ba2+, Mg2+ and Ca2+ could partially replace Mn2+, but Co2+, Fe2+, Cd2+ and Zn2+ could not. Sodium dodecyl sulphate, cetylpyridinium chloride, sodium deoxycholate, octyl beta-D-glucoside, digitonin and alkyl alcohols were less effective in enhancing enzyme activity, and dimethyl sulphoxide was ineffective. The apparent Michaelis constants were 1.25 mM for UDP-N-acetylglucosamine, 0.94-3.34 mM for freezing-point-depressing glycoprotein and 0.19 mM for periodate-treated blood-group-A porcine submaxillary mucin. Asialo ovine submaxillary mucin could not serve as the glycosyl acceptor. The structure of the 14C-labelled oligosaccharide obtained by alkaline-borohydride treatment of the product was identified as Gal beta 1----3(Glc-NAc beta 1----6)N-acetylgalactosaminitol by beta-hexosaminidase treatment, gas chromatography-mass spectrometry and 1H-n.m.r. (270 MHz) analysis. The enzyme is important in the regulation of mucin oligosaccharide biosynthesis.
Shock Wave Polarizations and Optical Metrics in the Born and the Born-Infeld Electrodynamics
Creators: name: Minz, Christoph
name: von Borzeszkowski, Horst-Heino
name: Chrobok, Thoralf
name: Schellstede, Gerold
Publisher: name: arXiv
Year: 2014
Subjects: subject: Mathematical Physics (math-ph)
subject: FOS: Physical sciences

description: We analyze the behavior of shock waves in nonlinear theories of electrodynamics. For this, by use of generalized Hadamard step functions of increasing order, the electromagnetic potential is developed in a series expansion near the shock wave front. This brings about a corresponding expansion of the respective electromagnetic field equations which allows for deriving relations that determine the jump coefficients in the expansion series of the potential. We compute the components of a suitable gauge-normalized version of the jump coefficients given for a prescribed tetrad compatible with the shock front foliation. The solution of the first-order jump relations shows that, in contrast to linear Maxwell's electrodynamics, in general the propagation of shock waves in nonlinear theories is governed by optical metrics and polarization conditions describing the propagation of two differently polarized waves (leading to a possible appearance of birefringence). In detail, shock waves are analyzed in the Born and Born-Infeld theories verifying that the Born-Infeld model exhibits no birefringence and the Born model does. The obtained results are compared to those ones found in literature. New results for the polarization of the two different waves are derived for Born-type electrodynamics.
description: 13 pages, 1 figure
Global implementation research capacity building to address cardiovascular disease: An assessment of efforts in eight countries
Creators: name: Price, LeShawndra N.
name: Ramirez-Zea, Manuel
name: Weber, Mary Beth
name: Andesia, Josephine
name: Apusiga, Kingsley
name: Rakhra, Ashlin
name: Fitzpatrick, Annette L.
name: Muula, Adamson S.
name: Nguyen, Hoa L.
name: Ha, Duc A.
name: Fort, Meredith P.
name: Hosseinipour, Mina C.
name: Akwanalo, Constantine
name: Adjei, Kezia Gladys Amaning
name: Baumann, Ana A.
Publisher: name: Public Library of Science
Year: 2023

description: Cardiovascular diseases are the leading causes of morbidity and mortality worldwide, but implementation of evidence-based interventions for risk factors such as hypertension is lacking, particularly in low and middle income countries (LMICs). Building implementation research capacity in LMICs is required to overcome this gap. Members of the Global Research on Implementation and Translation Science (GRIT) Consortium have been collaborating in recent years to establish a research and training infrastructure in dissemination and implementation to improve hypertension care. GRIT includes projects in Ghana, Guatemala, India, Kenya, Malawi, Nepal, Rwanda, and Vietnam. We collected data from each site on capacity building activities using the Potter and Brough (2004) model, mapping formal and informal activities to develop (a) structures, systems and roles, (b) staff and infrastructure, (c) skills, and (d) tools. We captured information about sites’ needs assessments and metrics plus program adaptations due to the COVID-19 pandemic. All sites reported capacity building activities in each layer of the Capacity Pyramid, with the largest number of activities in the Skills and Tools categories, the more technical and easier to implement categories. All sites included formal and informal training to build Skills. All sites included a baseline needs assessment to guide capacity building activities or assess context and inform intervention design. Sites implementing evidence-based hypertension interventions used common implementation science frameworks to evaluate implementation outcomes. Although the COVID-19 pandemic affected timelines and in-person events, all projects were able to pivot and carry out planned activities. Although variability in the activities and methods used existed, GRIT programs used needs assessments to guide locally appropriate design and implementation of capacity building activities. COVID-19 related changes were necessary, but strong collaborations and relationships with health ministries were maintained. The GRIT Consortium is a model for planning capacity building in LMICs.
POSSIBILITY OF APPLICATION OF THE NANOCOMPOSITES CONSISTING OF QUANTUM DOTS WITH THE PULSED LASER BEAM RADIATION IN NANOMEDICINE
Creators: name: Khvedelidze Leonardo
Publisher: name: Международный журнал гуманитарных и естественных наук
Year: 2023
Subjects: NANOPARTICLES
NANOMATERIALS
NANOMEDICINE
PHYSICAL MODEL

description: This paper discusses possibilities of using quantum dots in nanomedicine. The physical model is given, on the basis of which the relevant conclusions are made. The issue is equally important from both theoretical and practical points of view.
Eigenvalues enclosures of skew symmetric/Hermitian matrices having bounded uncertainty
Creators: name: Maiti, Suman
name: Chakraverty, Snehashish
Publisher: name: Hannover : Gottfried Wilhelm Leibniz Universität
Year: 2022
Subjects: subject: Skew symmetric/Hermitian interval matrices
subject: Eigenvalues bounds
subject: Konferenzschrift
subject: Dewey Decimal Classification::500 | Naturwissenschaften::510 | Mathematik

description: [no abstract available]
Pertussis toxin does not inhibit muscarinic-receptor-mediated phosphoinositide hydrolysis or calcium mobilization
Creators: name: Martin, M W
name: Masters, S B
name: Harden, T K
name: Brown, J H
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1985

description: Pertussis toxin was used to examine the role of the inhibitory guanine nucleotide regulatory protein, Ni, in muscarinic-receptor-mediated stimulation of phosphoinositide turnover and calcium mobilization. In cultured chick heart cells, pertussis-toxin treatment inhibited muscarinic-receptor-mediated attenuation of isoprenaline-stimulated cyclic AMP accumulation. This finding is consistent with the proposal that pertussis toxin blocks the capacity of Ni to couple muscarinic receptors to adenylate cyclase. In contrast, treatment of chick heart cells or 1321N1 human astrocytoma cells with pertussis toxin did not block muscarinic-receptor-mediated stimulation of phosphoinositide hydrolysis, as measured by [3H]inositol phosphate accumulation in the presence of Li+. Pertussis-toxin treatment also had little effect on basal and muscarinic-receptor-stimulated phosphatidylinositol synthesis, as measured by the incorporation of [3H]inositol into phosphatidylinositol. Activation of muscarinic receptors also enhances the rate of unidirectional 45Ca2+ efflux in 1321N1 cells; this response, like phosphoinositide hydrolysis, was not prevented by pertussis-toxin treatment. Our data suggest that muscarinic receptors are not coupled to phosphoinositide hydrolysis or calcium mobilization through Ni.
Grundlagen zur automatischen intrinsischen metabolischen Defibrillation von ventrikulären Herzrhythmusstörungen mittels säuresensitiver Ionenkanäle
Creators: name: Pantenburg, Jakob Friedemann
Publisher: name: Universitäts- und Landesbibliothek Bonn
Year: 2024
Subjects: subject: ventrikuläre Tachykardie
subject: Defibrillation
subject: ASICS
subject: Herzrhythmusstörung
subject: Herzphysiologie
subject: Kardiologie
subject: AIMD
subject: Kardiomyozyten
subject: ddc:610

description: Diese Studie untersucht die Möglichkeit, überexprimierte säuresensitive Kationenkanäle (ASICs) in Kardiomyozyten als Werkzeug zur selbständigen Beendigung hämodynamisch relevanter Herzrhythmusstörungen wie ventrikuläre Tachykardien zu nutzen. Die Ergebnisse legen die Grundlage für das innovative Konzept der automatisch intrinsisch metabolischen Defibrillation. Dieser alternative Methode zur Terminierung ventrikulärer Tachykardien würde keinen Einsatz implantierbarer Defibrillatoren erfordern.
بازنمایی استعاری جهان های زیرشمول در انگارۀ جهان های متن از منظر شناختی: مطالعۀ موردی اشعار شیرکو بیکس در سرآغاز کتاب کرسی
Publisher: name: دانشگاه کردستان
Year: 2022

description: جُستار حاضر با هدف بازنمایی استعاری لایۀ معنایی جهان های زیرشمول در انگارۀ جهان های متن ورث (1999) به بررسی کرسی اثر شیرکو بیکس از منظر رویکرد شناختی پرداخته است. در این پژوهش، ماهیت مناظر شعری شیرکو بیکس به  لحاظ زبانی و همچنین تعیین نوع منظر و دیدگاه وی مورد بررسی قرار گرفته است. ماهیت روش انجام این پژوهشِ کیفی، توصیفی-تحلیلی بوده و داده ها به روش کتابخانه ای از کتاب کرسی اثر شیرکو بیکس گردآوری شده اند. نگارندگان، همچنین، قصد دارند تا نشان دهند که چگونه مناظر ادبی شعر شیرکو بیکس، وسیلة بیان افکار و ذهنیات او در سرآغاز کتاب کرسی بوده است. بنابراین، با تکیه بر مفهوم استعاره در انگاره جهان های متن به رمزگشایی از جهان های استعاری این شاعر کُردزبان در سرآغاز کتاب کرسی به منظور کشف نگرش پدیدآورندة اثر پرداخته شد. نتایج نشان داد که کلان استعارة زیرساختی بیرون به  مثابۀ درون از رهگذر خُرداستعاره های روساختی باد، شعر، قصه و نمایشنامه که به  عنوان انسان هستند، بازنمایی می شوند.\n
Stabilizing Controller Design Using an Iterative LMIs Approach for Quadrotors
Creators: name: Benziane, Oussama
name: Rauh, Andreas
Publisher: name: Hannover : Gottfried Wilhelm Leibniz Universität
Year: 2022
Subjects: subject: Interval methods
subject: Linear matrix inequalities
subject: Robust control
subject: Konferenzschrift
subject: Dewey Decimal Classification::500 | Naturwissenschaften::510 | Mathematik

description: [no abstract available]
Hepatitis B care cascade among people with HIV/HBV coinfection in the North American AIDS Cohort Collaboration on Research and Design, 2012-2016
Creators: name: Napravnik S.
name: Torgersen J.
name: Moore R.D.
name: Lim J.K.
name: Horberg M.A.
name: Kim J.
name: Lang R.
name: Klein M.B.
name: McGinnis K.A.
name: Sterling T.R.
name: Kirk G.D.
name: Newcomb C.W.
name: Silverberg M.J.
name: Chew K.
name: Reddy K.R.
name: Gill M.J.
name: Carbonari D.M.
name: Lo Re V., 3rd
name: Gebo K.A.
name: Hull M.
name: Coburn S.B.
name: Mayor A.M.
name: Cachay E.R.
name: Althoff K.N.
name: Seaberg E.C.
name: Kim H.N.
name: Kitahata M.M.
Publisher: name: Public Library of Science
Year: 2023

description: A care cascade is a critical tool for evaluating delivery of care for chronic infections across sequential stages, starting with diagnosis and ending with viral suppression. However, there have been few data describing the hepatitis B virus (HBV) care cascade among people living with HIV infection who have HBV coinfection. We conducted a cross-sectional study among people living with HIV and HBV coinfection receiving care between January 1, 2012 and December 31, 2016 within 13 United States and Canadian clinical cohorts contributing data to the North American AIDS Cohort Collaboration on Research and Design (NA-ACCORD). We evaluated each of the steps in this cascade, including: 1) laboratory-confirmed HBV infection, 2) tenofovir-based or entecavir-based HBV therapy prescribed, 3) HBV DNA measured during treatment, and 4) viral suppression achieved via undetectable HBV DNA. Among 3,953 persons with laboratory-confirmed HBV (median age, 50 years; 6.5% female; 43.8% were Black; 7.1% were Hispanic), 3,592 (90.9%; 95% confidence interval, 90.0-91.8%) were prescribed tenofovir-based antiretroviral therapy or entecavir along with their antiretroviral therapy regimen, 2,281 (57.7%; 95% confidence interval, 56.2-59.2%) had HBV DNA measured while on therapy, and 1,624 (41.1%; 95% confidence interval, 39.5-42.6) achieved an undetectable HBV DNA during HBV treatment. Our study identified significant gaps in measurement of HBV DNA and suppression of HBV viremia among people living with HIV and HBV coinfection in the United States and Canada. Periodic evaluation of the HBV care cascade among persons with HIV/HBV will be critical to monitoring success in completion of each step.
Differential effect of glucagon on gluconeogenesis in periportal and pericentral regions of the liver lobule
Creators: name: Kinugasa, A
name: Thurman, R G
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1986

description: The effect of glucagon on gluconeogenesis was measured in periportal and pericentral regions of the liver lobule by monitoring changes in rates of O2 uptake on the surface of the perfused liver with miniature O2 electrodes after infusion of lactate. When lactate (2 mM) was infused into livers from starved rats perfused in the anterograde direction, O2 uptake was increased 2.5-fold more in periportal than in pericentral regions, reflecting increased energy demands for glucose synthesis. Under these conditions, glucagon infusion in the presence of lactate increased O2 uptake exclusively in periportal regions of the liver lobule. Thus, when perfusion is in the physiological anterograde direction, the metabolic actions of glucagon predominate in periportal regions of the liver lobule under gluconeogenic conditions in the starved state. When livers were perfused in the retrograde direction, however, glucagon stimulated O2 uptake exclusively in pericentral regions. Thus glucagon only stimulates gluconeogenesis in 'upstream' regions of the liver lobule irrespective of the direction of flow.
Comparing Monte Carlo methods for finding ground states of Ising spin glasses: population annealing, simulated annealing and parallel tempering
Creators: name: Wang, Wenlong
name: Machta, Jonathan
name: Katzgraber, Helmut G.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Disordered Systems and Neural Networks (cond-mat.dis-nn)
subject: FOS: Physical sciences

description: Population annealing is a Monte Carlo algorithm that marries features from simulated annealing and parallel tempering Monte Carlo. As such, it is ideal to overcome large energy barriers in the free-energy landscape while minimizing a Hamiltonian. Thus, population annealing Monte Carlo can be used as a heuristic to solve combinatorial optimization problems. We illustrate the capabilities of population annealing Monte Carlo by computing ground states of the three-dimensional Ising spin glass with Gaussian disorder, whilst comparing to simulated annealing and parallel tempering Monte Carlo. Our results suggest that population annealing Monte Carlo is significantly more efficient than simulated annealing but comparable to parallel tempering Monte Carlo for finding spin-glass ground states.
description: 9 pages, 7 figures
مشروعیت بخشی و مشروعیت زدایی در اشعار ملک الشعرا بهار و حاجی  قادر کویی بر اساس نظریه ی ون لیوون
Publisher: name: دانشگاه کردستان
Year: 2022

description: تحلیل گفتمان انتقادی، آشکار ساختن افکار و ایدئولوژی های پنهان در یک متن (گفتمان) است. یکی از نظریه پردازان تحلیل گفتمان انتقادی «تئو ون لیوون» است که نظریه ی «کنشگران اجتماعی» و «کنش - واکنش های اجتماعی» او در پی مشروعیت بخشی یا مشروعیت زدایی برخی کنشگران، کردارها و اهداف آنان است. بنابراین شیوه های مشروعیت بخشی به یک امر یا مشروعیت زدایی از آن، اساس این نظریه است. چهار راهکار اصلی مشـروعیت بخشی و مشروعیت زدایی به گفتمان عبارت است از: 1-اعتباربخشی، 2- ارزش گذاری اخلاقی، 3- عقلانی کردن و 4- اسطوره سازی. هریک از این چهار شیوه، اشکال متنوعی دارند و در این پژوهش، اشعار ملک الشعرا بهار و حاجی  قادر کویی از دیدگاه شیوه های مشروعیت بخشی و مشروعیت زدایی به گفتمان بررسی شده است. یافته های پژوهش نشان می دهد که بسامد مشروعیت زدایی در اشعار بهار و حاجی  قادر کویی بیشتر از مشروعیت بخشی است. الگوهای عمل در اشعار بهار و حاجی قادر، قرآن و آیات قرآنی، شاهان باستانی و گذشته ی افتخارآمیز ایران باستان و نیز برخی کشورهای متمدن هستند. بهار و حاجی  قادر با ذکر سخنان پیامبر (ص) و بزرگان ایران باستان از طریق اعتبار بخشی، با الگو قرار دادن قرآن و بزرگان دینی و شاهان ایران باستان و انتقاد از کشور و اعضای هیأت حاکمه، از حکومت وقت مشروعیت زدایی کرده اند. همچنین هر دو به اسطوره های پهلوانی و قهرمانی ایران اشاره می کنند تا علاوه بر ایجاد روحیه ی مبارزه در مردم، از حکومت مشروعیت زدایی کرده باشند.\n
Symmetries for Interval Analysis
Creators: name: Jaulin, Luc
Publisher: name: Hannover : Gottfried Wilhelm Leibniz Universität
Year: 2022
Subjects: subject: Interval analysis
subject: Symmetries
subject: Contractors
subject: Konferenzschrift
subject: Dewey Decimal Classification::500 | Naturwissenschaften::510 | Mathematik

description: [no abstract available]
MPEC 2024-R279 : 2024 RF13
Creators: name: Minor Planet Center Staff
Publisher: name: The Minor Planet Center
Year: 2024
Subjects: subject: Small Solar System bodies
valueUri: http://astrothesaurus.org/uat/1469
subject: 2024 RF13

description: The Minor Planet Electronic Circulars contain information on unusual minor planets, routine data on comets and natural satellites, and occasional editorial announcements.  They are published on behalf of Division F of the International Astronomical Union by the Minor Planet Center, Smithsonian Astrophysical Observatory, Cambridge, MA 02138, U.S.A.
title: Apprendistato,  discriminazione e razzismi quotidiani  in Alto Adige
Creators: name: Mitterhofer, Johanna
name: Obukhova, Kseniia
Publisher: name: Eurac Research
publisherIdentifier: https://ror.org/01xt1w755
publisherIdentifierScheme: ROR
Year: 2024

description: Questa ricerca è una prima esplorazione della tematica delle esperienze di discriminazione di apprendisti nella Provincia autonoma di Bolzano. Attinge a dati qualitativi e quantitativi raccolti tramite un questionario, delle interviste semi strutturate, e dei workshop interattivi con apprendisti ed esperti del settore della scuola, la formazione professionale, l’antidiscriminazione e il mondo del lavoro condotti nel 2023-2024. Dalle risposte raccolte emergono vari spunti da approfondire in successivi lavori di ricerca ed analisi: da una parte le persone intervistate riportano pochi atti espliciti di discriminazione e razzismo – un risultato sicuramente positivo. Dall’altra però evidenziano che episodi di razzismo quotidiano e micro-aggressioni, così come la discriminazione strutturale, sono fenomeni presenti in tutti gli ambienti frequentati dagli apprendisti e dalle apprendiste, ovvero sia nelle scuole che sul posto del lavoro.
بررسی استحاله شخصیت ها به باد در دو رمان کیمیاگر و جمشید خان عمویم از منظر رئالیسم جادویی
Publisher: name: دانشگاه کردستان
Year: 2022

description: رئالیسم جادویی با مولفه های خاص خود به ویژه وقوع امر جادویی باورپذیر از ژانر  های پرطرفدار ادبیات داستانی جهان، هم برای نویسندگان و هم برای مخاطبین، است. ساختارها، مولفه ها و فرم های رئالیسم جادویی به صورت آگاهانه و به تاثیر یا به صورت ناخودآگاه در آثار نویسندگان مختلف به شیوه های گوناگون پرورش داده شده و پرداخت شده اند. در این پژوهش رمان کیمیاگر اثر پائولو کوئلیو و رمان جمشید خان عمویم، که باد همیشه او را با خود می برد، نوشته ی نویسنده کُرد معاصر بختیار علی، از منظر مکتب آمریکایی مورد تطبیق قرار می گیرند تا وجوه تمایز و شباهت آن ها تحلیل و بررسی شود. ارتباط شخصیت های اصلی هر دو رمان با عنصر باد در چهارچوب رئالیسم جادویی اتفاق می افتد و نقش بسزایی در شکل گیری ساختار و محتوای آنها ایفا می کند، و در نهایت منجر به پرورش روایاتی منحصربفرد می گردد که مخاطب را در فضاهای متمایز با خود همراه می کند.\n
Fault Detection in Networked Control Systems. A Robust Approach
Creators: name: Valero, Carlos
name: Paulen, Radoslav
Publisher: name: Hannover : Gottfried Wilhelm Leibniz Universität
Year: 2022
Subjects: subject: Set-membership state estimation
subject: fault detection
subject: zonotopes
subject: Konferenzschrift
subject: Dewey Decimal Classification::500 | Naturwissenschaften::510 | Mathematik

description: [no abstract available]
Hydryphantes (Papilloporus) incertus Koenike 1893
Creators: name: Pešić, Vladimir
name: Chatterjee, Tapas
name: Bordoloi, Sabitry
Publisher: name: Zenodo
Year: 2010
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Arachnida
Trombidiformes
Hydryphantidae
Hydryphantes
Hydryphantes incertus

description: Hydryphantes (Papilloporus) incertus Koenike, 1893
New records: India, Assam State: Kamrup district, SW of Guwahati city, Deeper Beel wetland, between the water hyacinth Eichhornia corniculata, 26.10903N 91.68359E, 37 m asl., 8. xii. 2009, leg. Das & Bordoloi, 0/1/1; Basistha stream, leg. Bordoloi, 0/1/0.
Remarks: New for the fauna of India.
Habitat: Ponds, pools of streams.
Distribution. Africa, SE Asia.
description: Published as part of Pešić, Vladimir, Chatterjee, Tapas & Bordoloi, Sabitry, 2010, A checklist of the water mites (Acari: Hydrachnidia) of India, with new records and description of one new species, pp. 1-54 in Zootaxa 2617 (1) on page 6, DOI: 10.11646/zootaxa.2617.1.1, http://zenodo.org/record/197964
روابط بینامتنی منظومه های خسرو و شیرین، لیلی و مجنون و هفت پیکر نظامی با سه ‏منظومه ی عامیانه ‏کُردی (مم و زین، شیخ فرخ و خاتون استی، بارام و گل اندام)
Publisher: name: دانشگاه کردستان
Year: 2022

description: یکی از حوزه های مورد توجه در نقد ادبی، بررسی «روابط بینامتنی» آثار ادبی در یک یا چند زمینه فرهنگی و ادبی است؛ به ویژه اگر این زبان و فرهنگ ها دریک محدوده جغرافیایی و تاریخی مشترکی باشند این روابط برجسته تر بوده و به نتایج بهتری در نقد ادبی منجر خواهد شد. بر همین اساس در این پژوهش سعی شده است به روابط بینامتنی سه اثر نظامی با سه منظومة کردی مم و زین، شیخ فرخ و خاتون استی و منظومة بارام و گل اندام پرداخته شود و با مقایسة اشتراکات و اختلافات و بررسی چرایی آن ها به عوامل فرهنگی مؤثر بر اشتراکات، اشاره گردد و نشان داده شود چگونه متون حتی اگر در شکل متفاوت (مکتوب و شفاهی) باشند، می توانند در ساختار و محتوا از هم تأثیر بپذیرند. به ویژه در این مقاله تأکید بر تأثیر زبان مادری در پرداختن به محتوا و زبان در آثار است. این پژوهش با روش تطبیقی تحلیلی انجام گرفته است و نمونه های تحقیق عبارت اند از منظومه های خسرو و شیرین، لیلی و مجنون و هفت پیکر نظامی و سه ‏منظومه ی عامیانه ‏کُردی مم و زین، شیخ فرخ و خاتون استی و منظومة بارام و گل اندام. نتیجه پژوهش حاکی از این است که در بین آثار اشتراکاتی از قبیل نوع دلدادگی، آداب  و رسوم، عقاید و هنجارها و سرانجام قهرمانان دیده می شود که می تواند به صورت خودآگاه و ناخودآگاه در متن تبلوریافته و در ساختار و محتوای آن نمود یابد.\n
Herbal Remedies for Constipation-Predominant Irritable Bowel Syndrome: A Systematic Review of Randomized Controlled Trials
Creators: name: Popa, Stefan Lucian
name: Dumitrascu, Dinu Iuliu
name: Ismaiel, Abdulrahman
name: Duse, Traian Adrian
name: Surdea-Blaga, Teodora
name: Brata, Vlad Dumitru
name: Pop, Cristina
name: Incze, Victor
name: Chiarioni, Giuseppe
Publisher: name: MDPI
Year: 2023

description: Background: Constipation-predominant irritable bowel syndrome (IBS-C) is a common gastrointestinal disorder characterized by abdominal pain and altered bowel habits. Conventional treatments for IBS-C often provide limited efficiency, leading to an increasing interest in exploring herbal remedies. This systematic review aims to evaluate the efficacy and safety of herbal remedies in the management of IBS-C. Materials and Methods: A comprehensive search of PubMed, MEDLINE, Embase, Scopus, and the Cochrane Library was conducted to identify relevant studies published up to July 2023 and data extraction was performed independently by two reviewers. Results: Overall, the included studies demonstrated some evidence of the beneficial effects of herbal remedies on IBS-C symptoms, including improvements in bowel frequency, stool consistency, abdominal pain, and quality of life. However, the heterogeneity of the interventions and outcome measures limited the ability to perform a meta-analysis. Conclusion: This systematic review suggests that herbal remedies may have potential benefits in the management of IBS-C. However, the quality of evidence is limited, and further well-designed, large-scale RCTs are needed to establish the efficacy and safety of specific herbal remedies for IBS-C. Clinicians should exercise caution when recommending herbal remedies and consider individual patient characteristics and preferences.
واکاوی بسترهای فرهنگی و اجتماعی در گذار از نقد ادبی سنتی به نوین در ادبیات کردی در اواسط و اواخر سده بیستم
Publisher: name: دانشگاه کردستان
Year: 2022

description: نقد ادبی به معنی امروزی آن دانش و شاخه ای نوینی در ادبیات است که از بارقه­های آن تا رسیدن به مرحله ی شکوفایی و نقد بر پایه ی مبانی نظری، مسیری طولانی را طی کرده، و عوامل مختلفی در رسیدن به مرحله کنونی آن دخیل بوده اند. نقد ادبی از آبشخورهای آن که بیشتر نقد ضمن شعر و ناظر بر تحسین و تفاخر بوده تا دوره ی رونق، عوامل جانبی زیادی در رونق آن ایفای نقش کرده اند که نادیده گرفتن آنها سبب می شود سیمای کلی جریان نقد درست ترسیم نشود. این عوامل عبارتند از: نقش مجلات و روزنامه­ها، انتشارات و چاپخانه ها، نو شدن جریان شعر، توسعه گفتمان آکادمیک و گسترش انجمن های علمی، شاعران منتقد و نهضت های ادبی که هر کدام در ترسیم سیمای کلی نقد نقش مهمی داشته اند. این پژوهش با تحقیق در انواع آثار ادبی و در نظر گرفتن نقش هر کدام از جریان های فرهنگی و اجتماعی، عوامل تأثیرگذار در پیشرفت نقد ادبی را مشخص کرده است. در نتیجه ی تأثیر این عوامل راه ورود مباحث و نقدهای نو و مبانی نظری به نقد ادبی در ادبیات کُردی باز شد. مقاله ی  حاضر بر آن است مهمترین عوامل فرهنگی و اجتماعی در ظهور نقد ادبی نوین به زبان کُردی (میانه) در دو مقطع تاریخی اواسط و اواخر سده ی بیست را مورد بررسی قرار دهد. تفاوت پژوهش حاضر با پژوهش های پیشین مربوط به در نظر گرفتن سیمای کلی نقد و بررسی عوامل مؤثر در تغییر نقد سنتی به نقد نوین در ادبیات کُردی است.\n
title: Contrôle de la transmission du son par les murs en blocs de béton
Creators: name: Warnock, A. C. C.
Publisher: name: Conseil national de recherches du Canada. Institut de recherche en construction
Year: 1998
Subjects: subject: Walls
valueUri: urn:ircsubjects:IRC0842
subject: Murs
valueUri: urn:ircsubjects:IRC0842

description: With concrete block walls, sound insulation is mostly a function of their mass per unit area. With being heavy, such walls -- even single-leaf walls -- usually provide STC ratings of 50±5. (Double-leaf concrete block walls with their greater mass can potentially provide even more sound isolation.) Then sealing the surface of such walls with plaster, block sealer, or just paint, and sound transmission is even further reduced. Attaching gypsum board supported on furring or studs and using sound-absorbing materials can improve sound insulation considerably, depending very much on just how it is done.
description: Dans le cas des murs en blocs de béton, l'isolement acoustique dépend surtout de la masse par unité de surface. Parce qu'ils sont lourds, ces murs - même les simples - présentent habituellement un ITS de 50±5. (Les murs doubles en blocs de béton, qui ont une plus grande masse, peuvent assurer un isolement acoustique encore meilleur.) Appliquez un enduit comme du plâtre ou un protecteur, ou simplement de la peinture, sur la paroi, et la transmission du son est réduite davantage. La pose de plaques de plâtre sur des fourrures ou des poteaux et l'utilisation de matériaux insonorisants peuvent améliorer considérablement l'isolement acoustique, un facteur important étant la manière dont cela est fait.
Higher-Order Methods for Differential Inclusions
Creators: name: Collins, Pieter
name: Geretti, Luca
name: Villa, Tiziano
name: Zivanovic Gonzalez, Sanja
Publisher: name: Hannover : Gottfried Wilhelm Leibniz Universität
Year: 2022
Subjects: subject: Differential inclusions
subject: rigorous numerics
subject: Taylor models
subject: Konferenzschrift
subject: Dewey Decimal Classification::500 | Naturwissenschaften::510 | Mathematik

description: [no abstract available]
title: Pourquoi les maisons ont besoin de ventilation mécanique
Creators: name: Haysom, J. C.
name: Reardon, J. T.
Publisher: name: Conseil national de recherches du Canada. Institut de recherche en construction
Year: 1998
Subjects: subject: Ventilation
valueUri: urn:ircsubjects:IRC0642
subject: Ventilation
valueUri: urn:ircsubjects:IRC0642

description: A house needs a third of its air exchanged every hour. Owners of houses built before 1960 relied on air leakage through the building envelope for indoor/outdoor air exchanges. And they could, because houses then were leaky enough and wind or temperature difference saw to the necessary air movement. In the 1960s, houses became more airtight, and some were heated electrically (they did not even need a chimney). In the 1970s with its oil crisis, houses became even more airtight. With little air leakage through the building envelope, a mechanical ventilation system is required for moisture and pollutant removal. So, the question now is: how does one ventilate optimally?
description: Dans une maison, le tiers de l'air doit être renouvelé chaque heure. Les propriétaires de maisons construites avant 1960 comptaient sur la transparence à l'air de l'enveloppe du bâtiment pour réaliser les échanges d'air entre l'intérieur et l'extérieur. Ils pouvaient le faire car les maisons étaient alors assez perméables, et le vent et les écarts de température assuraient les mouvements d'air nécessaires. Au cours des années 60, les maisons sont devenues plus étanches à l'air, et certaines étaient chauffées à l'électricité (elles n'avaient même pas besoin de cheminée). Avec la crise de l'énergie, pendant les années 70, cette tendance s'est encore accentuée. Lorsque l'enveloppe du bâtiment n'est guère perméable à l'air, il faut doter la maison d'un système de ventilation mécanique pour contrôler l'humidité et chasser les polluants. Alors, la question est la suivante : quelle est la meilleure méthode de ventilation?
High Genetic Diversity of HIV-1 and Active Transmission Clusters among Male-to-Male Sexual Contacts (MMSCs) in Zhuhai, China
Creators: name: Huang, Shaoli
name: Hong, Zhongsi
name: Li, Jiarun
name: Cui, Mingting
name: Tang, Weiming
name: Zhou, Shuntai
name: Sun, Caijun
name: Huang, Huitao
name: Zhou, Yi
name: Lyu, Hang
name: Huang, Wenyan
name: Lin, Yixiong
Publisher: name: MDPI
Year: 2023

description: Monitoring genetic diversity and recent HIV infections (RHIs) is critical for understanding HIV epidemiology. Here, we report HIV-1 genetic diversity and RHIs in blood samples from 190 HIV-positive MMSCs in Zhuhai, China. MMSCs with newly reported HIV were enrolled from January 2020 to June 2022. A nested PCR was performed to amplify the HIV polymerase gene fragments at HXB2 positions 2604–3606. We constructed genetic transmission network at both 0.5% and 1.5% distance thresholds using the Tamura-Nei93 model. RHIs were identified using a recent infection testing algorithm (RITA) combining limiting antigen avidity enzyme immunoassay (LAg-EIA) assay with clinical data. The results revealed that 19.5% (37/190) were RHIs and 48.4% (92/190) were CRF07_BC. Two clusters were identified at a 0.5% distance threshold. Among them, one was infected with CRF07_BC for the long term, and the other was infected with CRF55_01B recently. We identified a total of 15 clusters at a 1.5% distance threshold. Among them, nine were infected with CRF07_BC subtype, and RHIs were found in 38.8% (19/49) distributed in eight genetic clusters. We identified a large active transmission cluster (n = 10) infected with a genetic variant, CRF79_0107. The multivariable logistic regression model showed that clusters were more likely to be RHIs (adjusted OR: 3.64, 95% CI: 1.51~9.01). The RHI algorithm can help to identify recent or ongoing transmission clusters where the prevention tools are mostly needed. Prompt public health measures are needed to contain the further spread of active transmission clusters.
title: Façons actuelles d’aborder la ventilation mécanique des habitations
Creators: name: Haysom, J. C.
name: Reardon, J. T.
Publisher: name: Conseil national de recherches du Canada. Institut de recherche en construction
Year: 1998
Subjects: subject: Ventilation
valueUri: urn:ircsubjects:IRC0642
subject: Ventilation
valueUri: urn:ircsubjects:IRC0642

description: In many ways the 1995 National Building Code (NBC) parallels and complements the CSA standard CAN/CSA-F326 in defining current approaches to mechanical ventilation in houses. Whereas F326 does not stipulate a maximum sound output, the NBC, for example, does: 53 decibels. The NBC gives details about the interrelationship of air intake and air output and the role various types of fans must play in houses that are either with or without a forced-air heating system. Houses without a forced-air heating system can also present problems in the even distribution of outdoor air.
description: Par bien des aspects, le Code national du bâtiment (CNB) de 1995 est à la fois parallèle et complémentaire à la norme F326 de la CSA qui définit les méthodes actuelles de ventilation mécanique des maisons. Alors que la norme F326 ne fixe pas de niveau sonore maximum, le CNB quant à lui en prescrit un de 53 décibels. Le CNB décrit en détail les relations entre les entrées et sorties d'air ainsi que le rôle que doivent jouer les différents types de ventilateurs dans les maisons qui sont équipées ou non de système de chauffage à air pulsé. La distribution d'air frais est parfois un problème pour les maisons qui ne sont pas chauffées par air pulsé.
title: Isolement acoustique et résistance au feu des ensembles comportant des coupe-feu
Creators: name: Nightingale, T. R. T.
name: Sultan, M. A.
Publisher: name: Conseil national de recherches du Canada. Institut de recherche en construction
Year: 1998
Subjects: subject: Walls
valueUri: urn:ircsubjects:IRC0484
subject: Murs
valueUri: urn:ircsubjects:IRC0484
subject: Floors
valueUri: urn:ircsubjects:IRC0483
subject: Planchers
valueUri: urn:ircsubjects:IRC0483
subject: Flanking
valueUri: urn:ircsubjects:IRC0830
subject: Transmission indirecte
valueUri: urn:ircsubjects:IRC0830

description: When dealing with two interrelated parameters, here sound isolation and fire resistance, the challenge is not to gain in one at the expense of the other. That challenge is easily met with Group 0 fire stops, as long as the cavity between two sets of studs has an air gap of 25 mm or less. There is no flanking transmission of sound because the cavity is filled with insulation. With Group 1 fire stops, defined by the soft materials at the floor/wall junction, all flanking transmission of sound is absorbed by them. For Group 2 and Group 3 fire stops, flanking transmission occurs through the fire stops, more so with Group 3.
description: Lorsque l'on prend en considération deux paramètres interdépendants tels que l'isolement acoustique et la résistance au feu, le premier défi est de ne pas compromettre l'un au bénéfice de l'autre. C'est possible grâce aux coupe-feu du groupe 0, pourvu que la lame d'air entre les deux jeux de poteaux de bois soit inférieure ou égale à 25 mm. Aucune voie de transmission indirecte du son n'est créée car le vide est comblé par l'isolant. Les coupe-feu du groupe 1, qui assurent la jonction mur-plancher grâce à des matériaux relativement mous, absorbent la transmission indirecte du son. Quant aux coupe-feu des groupes 2 et 3, ils constituent, notamment ceux du groupe 3, une voie de transmission indirecte.
Identification and purification from bovine brain of a guanine-nucleotide-binding protein distinct from G s , G i and G o
Creators: name: Evans, T
name: Martin, M W
name: Harden, T K
name: Northup, J K
name: Waldo, G L
name: Fraser, E D
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1987

description: A guanine-nucleotide-binding protein (G-protein) was purified from cholate extracts of bovine brain membranes by sequential DEAE-Sephacel, Ultrogel AcA-34, heptylamine-Sepharose and Sephadex G-150 chromatography. Guanosine 5'-[gamma-[35S]thio]triphosphate (GTP[35S])-binding activity copurified with a 25,000 Da peptide and a 35,000-36,000 Da protein doublet. Neither pertussis toxin nor cholera toxin catalysed the ADP-ribosylation of a protein associated with the GTP[35S]-binding activity. Photoaffinity labelling of the purified protein with 8-azido[gamma-32P]GTP indicated that the GTP-binding site resides on the 25,000 Da protein. The 35,000-36,000 Da protein doublet was electrophoretically indistinguishable from the beta-subunits of other GTP-binding proteins, and the 36,000 Da protein was recognized by antiserum to oligomeric Gt. The purified protein specifically bound 17.2 nmol of GTP[35S]/mg of protein. The Kd of the binding site for radioligand was approx. 15 nM. The brain GTP-binding protein co-migrated during SDS/polyacrylamide-gel electrophoresis with a GTP-binding protein, named Gp, purified from human placenta [Evans, Brown, Fraser & Northup (1986) J. Biol. Chem. 261, 7052-7059], and cross-reacted with antiserum raised against the placental protein, but not with antiserum raised to brain Go. SDS/polyacrylamide-gel electrophoresis of the brain and placental GTP-binding proteins in the presence of Staphylococcus aureus V8 protease yielded identical peptide maps.
HIV-1 Infection Results in Sphingosine-1-Phosphate Receptor 1 Dysregulation in the Human Thymus
Creators: name: Kim, Shawn J.
name: Salvatore, Bradley
name: Vatakis, Dimitrios N.
name: Uittenbogaart, Christel H.
name: Gordon, Brent R.
name: Blom, Bianca
name: Resop, Rachel S.
Publisher: name: MDPI
Year: 2023

description: Regeneration of functional naïve T lymphocytes following the onset of human immunodeficiency virus (HIV) infection remains a crucial issue for people living with HIV (PLWH), even when adhering to antiretroviral therapy (ART). Thus far, reports on the impact of HIV-1 infection on the entry of thymic precursors and the egress of functional naïve T lymphocytes to and from the thymus are limited. We examined the impact of HIV-1 on Sphingosine-1-phosphate (S1P) signaling, which governs the egress of functional naïve thymocytes from the thymus to the periphery. Using in vitro experiments with primary human thymocytes and in vivo and ex vivo studies with humanized mice, we show that HIV-1 infection results in upregulation of the expression of S1P receptor 1 (S1PR1) in the human thymus. Intriguingly, this upregulation occurs during intrathymic infection (direct infection of the human thymic implant) as well as systemic infection in humanized mice. Moreover, considering the dysregulation of pro- and anti-inflammatory cytokines in infected thymi, the increased expression of S1PR1 in response to in vitro exposure to Interferon-Beta (IFN-β) and Tumor Necrosis Factor-Alpha (TNF-α) indicates that cytokine dysregulation following HIV infection may contribute to upregulation of S1PR1. Finally, an increased presence of CD3hiCD69− (fully mature) as well as CD3hiCD69+ (less mature) T cells in the spleen during HIV infection in humanized mice, combined with earlier expression of S1PR1 during thymocyte development, suggests that upregulation of S1PR1 may translate to increased or accelerated egress from the thymus. The egress of thymocytes that are not functionally mature from the thymus to peripheral blood and lymphoid organs may have implications for the immune function of PLWH.
بررسی سوره ملک و قلم در ترجمه منظوم ریبوار هه جمنه یی براساس نظریه یوجین نایدا
Publisher: name: دانشگاه کردستان
Year: 2022

description: قرآن کریم با ذات محتوایی و پیامی خود جهت هدایت و تغییر رفتار و سبک زندگی مادی­گرایانه به الهی­گونه بر بشر نازل شده است، چنین متن­هایی با متن نوشته­های بشری تفاوت­های اساسی دارد؛ بنابراین ترجمه این­گونه متن­ها با متن­های دیگر متفاوت است، مترجمان قرآن می کوشند تا بر اساس نظریه­های مطرح ترجمه به انتقال مفاهیم اصلی متن قرآن به زبان مقصد بپردازند به طوری که در کنار آن ساختار اصلی حفظ شود و ساخت نحوی و دستوری آن به زبان مقصد منتقل شود و معانی اصلی واژگان و عبارات زبان مبدأ در ترجمة منعکس گردد. در این پژوهش بر اساس روش تحلیل علمی و با تکیه بر عناصر اصلی ترجمه در نظریة یوجین نایدا، به بررسی ترجمة سوره­های «ملک» و «قلم» در ترجمة منظوم ریبوار هه جمنه یی به زبان کردی می پردازیم. نتیجة پژوهش نشان می­دهد که این ترجمه دارای نقاط قوت و ضعف است؛ چه این که مترجم در اثر خود از ترجمة صوری بهرة بسیاری برده و توانسته است زبان مقصد را به زبان مبدأ نزدیک سازد. از سوی دیگر، بنا بر افزایش متن ترجمه نسبت به متن آیات در برخی از ابیات، از ترجمة پویا در زبان مقصد بهره برده است. البته بیشتر معادل های مترجم در ترجمه صحیح است و این نشان دهندة تسلط وی به ترجمه است. هرچند ناپخته بودن متن مقصد در مواردی کاملا محرز بوده و عدم معادل­یابی نیز آشکار است. نهایت آن که ترجمة هه­جمنه­یی در قالب منظوم، ترجمه­ای قابل قبول و متناسب با زبان متن مبدأ است.\n
Host range, transmissibility and antigenicity of a pangolin coronavirus
Creators: name: Mattocks M.
name: Meganck R.M.
name: Yount B.
name: Kawaoka Y.
name: Tata P.R.
name: Powers J.
name: Martinez D.R.
name: West A.
name: Leist S.R.
name: Zost S.J.
name: Halfmann P.
name: Sontake V.
name: Catanzaro N.J.
name: Tata A.
name: Hou Y.J.
name: Adams L.
name: Crowe J.E., Jr.
name: Randell S.H.
name: Lee R.E.
name: Gallant S.C.
name: Baric R.S.
name: Schäfer A.
name: Chiba S.
name: Edwards C.E.
name: Kong E.F.
Publisher: name: Nature Research
Year: 2023

description: The pathogenic and cross-species transmission potential of SARS-CoV-2-related coronaviruses (CoVs) remain poorly characterized. Here we recovered a wild-type pangolin (Pg) CoV GD strain including derivatives encoding reporter genes using reverse genetics. In primary human cells, PgCoV replicated efficiently but with reduced fitness and showed less efficient transmission via airborne route compared with SARS-CoV-2 in hamsters. PgCoV was potently inhibited by US Food and Drug Administration approved drugs, and neutralized by COVID-19 patient sera and SARS-CoV-2 therapeutic antibodies in vitro. A pan-Sarbecovirus antibody and SARS-CoV-2 S2P recombinant protein vaccine protected BALB/c mice from PgCoV infection. In K18-hACE2 mice, PgCoV infection caused severe clinical disease, but mice were protected by a SARS-CoV-2 human antibody. Efficient PgCoV replication in primary human cells and hACE2 mice, coupled with a capacity for airborne spread, highlights an emergence potential. However, low competitive fitness, pre-immune humans and the benefit of COVID-19 countermeasures should impede its ability to spread globally in human populations.