title: ÐÐ·ÑÑÐµÐ½Ð¸Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¿Ð¾Ð»Ð¸Ð¼Ð¾ÑÑÐ¸Ð·Ð¼Ð° Ð½Ð° Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸Ð·Ð¼ Ð¼ÐµÑÑÐ¾ÑÐ¼Ð¸Ð½Ð° Ð¿ÑÐ¸ Ð»ÐµÑÐµÐ½Ð¸Ð¸ ÑÐ°Ñ Ð°ÑÐ½Ð¾Ð³Ð¾ Ð´Ð¸Ð°Ð±ÐµÑÐ° 2 ÑÐ¸Ð¿Ð°
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: Study of the genetic polymorphism effect on metformin metabolism in the treatment of type 2 diabetes
Creators: name: ÐÐµÐ²Ð·Ð¾ÑÐ¾Ð², ÐÐ²Ð°Ð½
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: Ð´Ð¸Ð°Ð±ÐµÑ Ð²ÑÐ¾ÑÐ¾Ð³Ð¾ ÑÐ¸Ð¿Ð°
Ð¼ÐµÑÑÐ¾ÑÐ¼Ð¸Ð½
SLC22Ð1
SLC47A1
AGBL1
COX7B2
type two diabetes mellitus
metformin

description: ÐÑÐ¾Ð²ÐµÐ´ÐµÐ½Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ðµ Ð°ÑÑÐ¾ÑÐ¸Ð°ÑÐ¸Ð¸ Ð¼ÐµÐ¶Ð´Ñ Ð¿Ð¾Ð»Ð¸Ð¼Ð¾ÑÑÐ½ÑÐ¼Ð¸ Ð²Ð°ÑÐ¸Ð°Ð½ÑÐ°Ð¼Ð¸ R61C Ð¸ 420del Ð³ÐµÐ½Ð° SLC22A1, Ð¸ rs2289669 Ð³ÐµÐ½Ð° SLC47A1 Ð¸ ÑÐ¾Ð»ÐµÑÐ°Ð½ÑÐ½Ð¾ÑÑÑÑ Ðº Ð¼ÐµÑÑÐ¾ÑÐ¼Ð¸Ð½Ñ Ð¿ÑÐ¸ Ð»ÐµÑÐµÐ½Ð¸Ð¸ Ð´Ð¸Ð°Ð±ÐµÑÐ° Ð²ÑÐ¾ÑÐ¾Ð³Ð¾ ÑÐ¸Ð¿Ð° Ð² Ð¿Ð¾Ð¿ÑÐ»ÑÑÐ¸Ð¸ Ð¡ÐµÐ²ÐµÑÐ¾-ÐÐ°Ð¿Ð°Ð´Ð½Ð¾Ð³Ð¾ ÑÐµÐ³Ð¸Ð¾Ð½Ð° Ð Ð¤. Ð¢Ð°ÐºÐ¶Ðµ Ð±ÑÐ»Ð¾ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð¾ Ð¿Ð¾Ð»Ð½Ð¾Ð³ÐµÐ½Ð¾Ð¼Ð½Ð¾Ðµ Ð³ÐµÐ½Ð¾ÑÐ¸Ð¿Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ ÑÐ¸Ð¿Ð¾Ð² GSA, Ñ ÑÐµÐ»ÑÑ Ð¾Ð¿ÑÐµÐ´ÐµÐ»Ð¸ÑÑ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ðµ Ð²Ð°ÑÐ¸Ð°Ð½ÑÑ, Ð°ÑÑÐ¾ÑÐ¸Ð¸ÑÐ¾Ð²Ð°Ð½Ð½ÑÐµ Ñ Ð²Ð¾Ð·Ð½Ð¸ÐºÐ½Ð¾Ð²ÐµÐ½Ð¸ÐµÐ¼/Ð¾ÑÑÑÑÑÑÐ²Ð¸ÐµÐ¼ Ð¾ÑÐ²ÐµÑÐ° Ð½Ð° ÑÐµÑÐ°Ð¿Ð¸Ñ Ð¼ÐµÑÑÐ¾ÑÐ¼Ð¸Ð½Ð¾Ð¼. Ð ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð¾Ð±ÑÐµÐºÑÐ° Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð° Ð²ÑÐ±ÑÐ°Ð½Ð° ÐÐÐ, Ð²ÑÐ´ÐµÐ»ÐµÐ½Ð½Ð°Ñ Ð¼ÐµÑÐ¾Ð´Ð¾Ð¼ ÑÐµÐ½Ð¾Ð»ÑÐ½Ð¾-ÑÐ»Ð¾ÑÐ¾ÑÐ¾ÑÐ¼Ð½Ð¾Ð¹ ÑÐºÑÑÑÐ°ÐºÑÐ¸Ð¸ Ð¸Ð· ÑÐµÐ»ÑÐ½Ð¾Ð¹ Ð²ÐµÐ½Ð¾Ð·Ð½Ð¾Ð¹ ÐºÑÐ¾Ð²Ð¸. Ð ÐºÐ°ÑÐµÑÑÐ²Ðµ Ð¼ÐµÑÐ¾Ð´Ð¾Ð² Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð»Ð¸ÑÑ: ÐÐ¦Ð , Ð¾Ð±ÑÐ°Ð±Ð¾ÑÐºÐ° ÑÐ½Ð´Ð¾Ð½ÑÐºÐ»ÐµÐ°Ð·Ð°Ð¼Ð¸ ÑÐµÑÑÑÐ¸ÐºÑÐ¸Ð¸, Ð²Ð¸Ð·ÑÐ°Ð»Ð¸Ð·Ð°ÑÐ¸Ñ Ð¿ÑÐ¾Ð´ÑÐºÑÐ¾Ð² Ð² Ð¿Ð¾Ð»Ð¸Ð°ÐºÑÐ¸Ð»Ð°Ð¼Ð¸Ð´Ð½Ð¾Ð¼ Ð³ÐµÐ»Ðµ, Ð¿Ð¾Ð»Ð½Ð¾Ð³ÐµÐ½Ð¾Ð¼Ð½Ð¾Ðµ Ð³ÐµÐ½Ð¾ÑÐ¸Ð¿Ð¸ÑÐ¾Ð²Ð°Ð½Ð¸Ðµ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ ÑÐ¸Ð¿Ð¾Ð² GSA, Ð° ÑÐ°ÐºÐ¶Ðµ Ð¼ÐµÑÐ¾Ð´Ñ Ð±Ð¸Ð¾Ð¸Ð½ÑÐ¾ÑÐ¼Ð°ÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð¸ ÑÑÐ°ÑÐ¸ÑÑÐ¸ÑÐµÑÐºÐ¾Ð³Ð¾ Ð°Ð½Ð°Ð»Ð¸Ð·Ð°. Ð ÑÐµÐ·ÑÐ»ÑÑÐ°ÑÐµ Ð²ÑÐ¿Ð¾Ð»Ð½ÐµÐ½Ð¸Ñ ÑÐ°Ð±Ð¾ÑÑ Ð±ÑÐ»Ð¸ Ð²Ð¿ÐµÑÐ²ÑÐµ Ð¿ÑÐ¾Ð°Ð½Ð°Ð»Ð¸Ð·Ð¸ÑÐ¾Ð²Ð°Ð½Ñ ÑÐ°ÑÑÐ¾ÑÑ Ð´Ð°Ð½Ð½ÑÑ Ð¿Ð¾Ð»Ð¸Ð¼Ð¾ÑÑÐ½ÑÑ Ð²Ð°ÑÐ¸Ð°Ð½ÑÐ¾Ð² Ð² Ð¿Ð¾Ð¿ÑÐ»ÑÑÐ¸Ð¸ Ð¡ÐµÐ²ÐµÑÐ¾-ÐÐ°Ð¿Ð°Ð´Ð½Ð¾Ð³Ð¾ ÑÐµÐ³Ð¸Ð¾Ð½Ð° Ð Ð¤. ÐÑÐ»Ð¾ Ð¿Ð¾ÐºÐ°Ð·Ð°Ð½Ð¾ Ð¾ÑÑÑÑÑÑÐ²Ð¸Ðµ ÑÐ²ÑÐ·Ð¸ Ð¼ÐµÐ¶Ð´Ñ Ð½Ð°Ð»Ð¸ÑÐ¸ÐµÐ¼ Ð²Ð°ÑÐ¸Ð°Ð½ÑÐ¾Ð² R61C Ð¸ 420del Ð³ÐµÐ½Ð° SLC22A1, Ð¸ rs2289669 Ð³ÐµÐ½Ð° SLC47A1 Ð¸ Ð¾ÑÑÑÑÑÑÐ²Ð¸ÐµÐ¼ ÑÑÑÐµÐºÑÐ° Ð¾Ñ Ð¿ÑÐ¸ÑÐ¼Ð° Ð¼ÐµÑÑÐ¾ÑÐ¼Ð¸Ð½Ð°. ÐÑÐ»Ð¸ Ð¾Ð±Ð½Ð°ÑÑÐ¶ÐµÐ½Ñ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸Ðµ Ð²Ð°ÑÐ¸Ð°Ð½ÑÑ, ÐºÐ¾ÑÐ¾ÑÑÐµ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»ÑÐ½Ð¾ Ð°ÑÑÐ¾Ð¸ÑÐ¸Ð¸ÑÐ¾Ð²Ð°Ð½Ñ Ñ Ð¾ÑÑÑÑÑÑÐ²Ð¸ÐµÐ¼ ÑÑÑÐµÐºÑÐ° Ð¾Ñ Ð¿ÑÐ¸ÑÐ¼Ð° Ð¼ÐµÑÑÐ¾ÑÐ¼Ð¸Ð½Ð°, Ð° Ð¸Ð¼ÐµÐ½Ð½Ð¾ rs7178601, rs78721842, rs28493319, rs28674959 Ð³ÐµÐ½Ð° AGBL1 Ð¸ rs17640893, rs73142962 Ð³ÐµÐ½Ð° COX7B2.
description: An association study was conducted between the polymorphic variants R61C and 420del of the SLC22A1 gene and rs2289669 of the SLC47A1 gene and metformin tolerance in the treatment of type 2 diabetes in a population of the North-West region of the Russian Federation. Genome-wide genotyping using GSA chips was also performed to determine the genetic variants associated with the occurrence/absence of a response to metformin therapy. Object of study was DNA isolated by phenol-chloroform extraction from whole venous blood. The research methods used: PCR, restriction endonuclease treatment, visualization of products in a polyacrylamide gel, genome-wide genotyping using GSA chips and bioinformatics and statistics methods. Results: the frequencies of the polymorphisms in the population of the North-West region of Russian Federation were first analyzed. No connection was shown between the presence of R61C and 420del polymorphisms of the SLC22A1 gene and rs2289669 of the SLC47A1 gene and the tolerance to metformin. Genetic variants that are potentially associated with the lack of the effect from taking metformin such as rs7178601, rs78721842, rs28493319, rs28674959 of the AGBL1 gene rs17640893, rs73142962 of the COX7B2 gene have been discovered.