cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
title: Private maritime Gewalt als außenpolitisches Instrument
Creators: name: Sarahrudi, Aria
Year: 2022

description: Die „Entdeckung“ Amerikas durch Christoph Kolumbus, ein aus globalhistorischer Perspektive zentrales Ereignis, stellte den Auftakt der europäischen Expansion in diesen Teil der Erde dar. Der dabei von den Königreichen Spanien und Portugal von Beginn an reklamierte Alleinherrschaftsanspruch in der sogenannten „Neuen Welt“ führte allerdings schon sehr bald zu Konflikten mit anderen seefahrenden europäischen Mächten, die diesem Begehren der Iberer keinerlei Geltung beimaßen. Dies traf auch auf England zu, wo man spätestens seit der Regierungszeit von Elisabeth I. damit begonnen hatte, die atlantische Expansion aktiv voranzutreiben. Organisiert wurde dieses Unterfangen in Anbetracht einer erst ab den 1620er Jahren allmählich entstehenden dauerhaften regulären englischen Kriegsmarine überwiegend mit Hilfe von privaten maritimen Gewaltakteuren in der Gestalt von Seebeutefahrern, die rasch vor allem die Karibik zum Hauptgebiet ihrer Aktivitäten machten, in geringerem Ausmaß aber ebenfalls in anderen Teilen der Neuen Welt präsent waren. Welche konkrete Rolle ihnen als unentbehrliche außenpolitische Instrumente jener Zeit beim Vordringen des englischen Königreiches in die Neue Welt zwischen 1562 und 1670 zukam, ist Gegenstand dieser Arbeit.
description: The “discovery” of the Americas by Christopher Columbus – a globally historic event – marked the beginning of European expansion into this part of the globe. From the very beginning the kingdoms of Spain and Portugal claimed their sole reign over the so-called “New World”, which soon led to conflicts with other European maritime nations that did not validate the Iberian claim. Among them was England, which actively pursued the Atlantic expansion since the reign of Queen Elizabeth I. Due to the non-existence of a permanent regular English Navy until the early 1620s, the venture was predominantly organized by actors of private maritime violence, who were authorized by a sovereign political power and whose main activities focused on the Caribbean, and to a lesser extent also on other parts of the New World. What role they played as indispensable foreign-policy instruments in the expansion of the English kingdom into the New World between 1562 and 1670 will be the topic of this paper.
Sonnenfleckenzeichnung Nr. 244.
Zeichnung von Flecken und Fackelflächen zur Ermittlung der Sonnenaktivität am 14.11.2012, 09:00, erstellt von Marco Cagnotti
Creators: name: Observatorium Specola Solare Ticinese Locarno (1957 bis 1980 Teil der Eidgenössischen Sternwarte der ETH Zürich)
Publisher: name: [s.n.]
Year: 2012

description: Bleistiftzeichnung mit schwarzer Farbe und Bleistift, erstellt als Projektionsbildzeichnung für die Beobachtungen in Locarno-Monti auf einem eigenen Vordruck. Instrument: Zeiss-Coudé-Refraktor mit 15 cm Öffnung und 225 cm Brennweite. Bildqualität (SIDC): 3.
Die Beobachtungen zu Einzelflecken und zu Sonnenfleckengruppen wurden auf dem Vordruck eingetragen. Zusätzlich sind zuhanden der späteren statistischen Verarbeitung die Angaben für die Bestimmung der Fleckenrelativzahl notiert.
Beobachtungstätigkeit unter eigenständiger Leitung (Associazione Specola Solare Ticinese ASST)
A Comprehensive Search for Dark Matter Annihilation in Dwarf Galaxies
Creators: name: Geringer-Sameth, Alex
name: Koushiappas, Savvas M.
name: Walker, Matthew G.
Publisher: name: arXiv
Year: 2014
Subjects: subject: Cosmology and Nongalactic Astrophysics (astro-ph.CO)
subject: Astrophysics of Galaxies (astro-ph.GA)
subject: High Energy Astrophysical Phenomena (astro-ph.HE)
subject: High Energy Physics - Phenomenology (hep-ph)
subject: Data Analysis, Statistics and Probability (physics.data-an)
subject: FOS: Physical sciences

description: We present a new formalism designed to discover dark matter annihilation occurring in the Milky Way's dwarf galaxies. The statistical framework extracts all available information in the data by simultaneously combining observations of all the dwarf galaxies and incorporating the impact of particle physics properties, the distribution of dark matter in the dwarfs, and the detector response. The method performs maximally powerful frequentist searches and produces confidence limits on particle physics parameters. Probability distributions of test statistics under various hypotheses are constructed exactly, without relying on large sample approximations. The derived limits have proper coverage by construction and claims of detection are not biased by imperfect background modeling. We implement this formalism using data from the Fermi Gamma-ray Space Telescope to search for an annihilation signal in the complete sample of Milky Way dwarfs whose dark matter distributions can be reliably determined. We find that the observed data is consistent with background for each of the dwarf galaxies individually as well as in a joint analysis. The strongest constraints are at small dark matter particle masses. Taking the median of the systematic uncertainty in dwarf density profiles, the cross section upper limits are below the pure s-wave weak scale relic abundance value (2.2 x 10^-26 cm^3/s) for dark matter masses below 26 GeV (for annihilation into b quarks), 29 GeV (tau leptons), 35 GeV (up, down, strange, and charm quarks and gluons), 6 GeV (electrons/positrons), and 114 GeV (two-photon final state). For dark matter particle masses less than 1 TeV, these represent the strongest limits obtained to date using dwarf galaxies.
description: 34 pages, 15 figures, a machine-readable table of observed cross section limits is available as an ancillary file, replaced with version to appear in Physical Review D
Sonnenfleckenzeichnung Nr. 280.
Zeichnung von Flecken und Fackelflächen zur Ermittlung der Sonnenaktivität am 31.12.2012, 09:00, erstellt von Sergio Cortesi
Creators: name: Observatorium Specola Solare Ticinese Locarno (1957 bis 1980 Teil der Eidgenössischen Sternwarte der ETH Zürich)
Publisher: name: [s.n.]
Year: 2012

description: Bleistiftzeichnung mit schwarzer Farbe und Bleistift, erstellt als Projektionsbildzeichnung für die Beobachtungen in Locarno-Monti auf einem eigenen Vordruck. Instrument: Zeiss-Coudé-Refraktor mit 15 cm Öffnung und 225 cm Brennweite. Bildqualität (SIDC): 3.
Die Beobachtungen zu Einzelflecken und zu Sonnenfleckengruppen wurden auf dem Vordruck eingetragen. Zusätzlich sind zuhanden der späteren statistischen Verarbeitung die Angaben für die Bestimmung der Fleckenrelativzahl notiert.
Beobachtungstätigkeit unter eigenständiger Leitung (Associazione Specola Solare Ticinese ASST)
The Zif268 cellular transcription factor activates expression of the Epstein-Barr virus immediate-early BRLF1 promoter.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1995

description: The Epstein-Barr virus immediate-early protein BZLF1 mediates the switch from latent to lytic infection. BZLF1 transcription can be derived from either the BZLF1 promoter or the BRLF1 promoter (Rp). Productive viral infection of EBV-infected B cells can be induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) treatment, as well as cross-linking of surface immunoglobulin with antiimmunoglobulin antibody. Both TPA and antiimmunoglobulin antibody are known to activate expression of the cellular transcription factor Zif268 in B cells. In this study, we have examined the regulation of BZLF1 transcription by Zif268. We show that Rp (but not the BZLF1 promoter) is activated by Zif268. Bacterially synthesized Zif268 binds strongly to an upstream sequence in the Rp promoter (located from -131 to -123 relative to the start site) and more weakly to a proximal sequence (-49 to -40). Zif268 activation of Rp requires these two Zif268 binding sites. TPA treatment of B cells induces the expression of Zif268 protein, which binds to Rp. Furthermore, TPA activation of Rp requires the upstream Zif268 site. These findings indicate that Zif268 can activate a critical Epstein-Barr virus immediate-early promoter and, therefore, may play a key role in the regulation of viral latency.
Iacobi Regnardi ... Teutsche Lieder, mit dreyen Stimmen, nach Art der neapolitanen oder welschen Villanellen : zuvor underschidlich in drey Theil aussgangen, an jetzt aber ... in ein Opus zusammen druckt
Teutsche Lieder, mit dreyen Stimmen, nach Art der neapolitanen oder welschen Villanellen
Deutsche Lieder Singst 3
Creators: name: Regnart, Jakob
Publisher: name: bey Adam Berg ...
Year: 1590

description: "3., unveränderte Ausg. des erstmals 1583 in derselben Offizin ersch. Druckes" -- W. Pass: Thematischer Katalog, S. 57
description: Discantus; Tenor; Bassus: je [2], LXVII, [3] S. -- Bassus: letzte gez. S. fälschlicherweise als XXVII bezeichnet
description: Titeleinfassung
Efficient long-term gene transfer into muscle tissue of immunocompetent mice by adeno-associated virus vector.
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1996

description: Muscle-directed gene transfer is being considered for the treatment of several metabolic diseases, including hemophilia and Duchene's muscular dystrophy. Previous efforts to target this tissue for somatic delivery with various vector systems have resulted in transient expression due to silencing of the transgene or to an immune response against the vector-transduced cells. We introduced recombinant adeno-associated virus vector (rAAV) carrying a lacZ reporter into muscle tissue of immunocompetent mice. The lacZ reporter gene was efficiently transduced and expressed with no evidence of a cellular immune response. Moreover, gene expression persisted for more than 1.5 years. Molecular characterization of rAAV vector DNA suggests a mechanism for persistence, since vector episomes convert to high-molecular-weight genomic DNA. These data provide the first report for establishing long-term gene transduction into mammalian muscle cells in vivo without the need for immune modulation of the organism.
Sonnenfleckenzeichnung Nr. 265.
Zeichnung von Flecken und Fackelflächen zur Ermittlung der Sonnenaktivität am 11.12.2012, 09:00, erstellt von Sergio Cortesi
Creators: name: Observatorium Specola Solare Ticinese Locarno (1957 bis 1980 Teil der Eidgenössischen Sternwarte der ETH Zürich)
Publisher: name: [s.n.]
Year: 2012

description: Bleistiftzeichnung mit schwarzer Farbe und Bleistift, erstellt als Projektionsbildzeichnung für die Beobachtungen in Locarno-Monti auf einem eigenen Vordruck. Instrument: Zeiss-Coudé-Refraktor mit 15 cm Öffnung und 225 cm Brennweite. Bildqualität (SIDC): 2.
Die Beobachtungen zu Einzelflecken und zu Sonnenfleckengruppen wurden auf dem Vordruck eingetragen. Zusätzlich sind zuhanden der späteren statistischen Verarbeitung die Angaben für die Bestimmung der Fleckenrelativzahl notiert.
Beobachtungstätigkeit unter eigenständiger Leitung (Associazione Specola Solare Ticinese ASST)
Trumpet Slices in Kerr Spacetimes
Creators: name: Dennison, Kenneth A.
name: Baumgarte, Thomas W.
name: Montero, Pedro J.
Publisher: name: arXiv
Year: 2014
Subjects: subject: General Relativity and Quantum Cosmology (gr-qc)
subject: FOS: Physical sciences

description: We introduce a new time-independent family of analytical coordinate systems for the Kerr spacetime representing rotating black holes. We also propose a (2+1)+1 formalism for the characterization of trumpet geometries. Applying this formalism to our new family of coordinate systems we identify, for the first time, analytical and stationary trumpet slices for general rotating black holes, even for charged black holes in the presence of a cosmological constant. We present results for metric functions in this slicing and analyze the geometry of the rotating trumpet surface.
description: 5 pages, 2 figures; version published in PRL
Membrane-Associated Heparan Sulfate Proteoglycan Is a Receptor for Adeno-Associated Virus Type 2 Virions
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1998

description: The human parvovirus adeno-associated virus (AAV) infects a broad range of cell types, including human, nonhuman primate, canine, murine, and avian. Although little is known about the initial events of virus infection, AAV is currently being developed as a vector for human gene therapy. Using defined mutant CHO cell lines and standard biochemical assays, we demonstrate that heparan sulfate proteoglycans mediate both AAV attachment to and infection of target cells. Competition experiments using heparin, a soluble receptor analog, demonstrated dose-dependent inhibition of AAV attachment and infection. Enzymatic removal of heparan but not chondroitin sulfate moieties from the cell surface greatly reduced AAV attachment and infectivity. Finally, mutant cell lines that do not produce heparan sulfate proteoglycans were significantly impaired for both AAV binding and infection. This is the first report that proteoglycan has a role in cellular attachment of a parvovirus. Together, these results demonstrate that membrane-associated heparan sulfate proteoglycan serves as the viral receptor for AAV type 2, and provide an explanation for the broad host range of AAV. Identification of heparan sulfate proteoglycan as a viral receptor should facilitate development of new reagents for virus purification and provide critical information on the use of AAV as a gene therapy vector.
Structural Localization of the E3 Glycoprotein in Attenuated Sindbis Virus Mutants
Creators: name: The University of North Carolina at Chapel Hill University Libraries
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 1998

description: We have determined the three-dimensional structures of the wild-type Sindbis virus and two of its mutants that retain the E3 sequence within PE2. Using difference imaging between these mutants and the wild-type virus, we have assigned a location for the 64-amino-acid sequence corresponding to E3 in the mutant spike complex. In the wild-type virus, the spike is composed of an E1-E2 heterotrimer. The E3 protein was found to protrude midway between the center of the spike complex and the tips. Based on these results and the work of others, we propose a distribution for the functional domains of the spike proteins within the structure of wild-type Sindbis virus. Within the structure of the virus, the E1 domains form the central portion of the spike complex, while the tips are formed by the E2 domains that flare out from the center of the complex. The structural similarity between these Sindbis virus mutants and Ross River virus suggests that E3 may also be present in the latter, which is also a member of the Alphavirus genus.
Sonnenfleckenzeichnung Nr. 249.
Zeichnung von Flecken und Fackelflächen zur Ermittlung der Sonnenaktivität am 19.11.2012, 10:00, erstellt von Sergio Cortesi
Creators: name: Observatorium Specola Solare Ticinese Locarno (1957 bis 1980 Teil der Eidgenössischen Sternwarte der ETH Zürich)
Publisher: name: [s.n.]
Year: 2012

description: Bleistiftzeichnung mit schwarzer Farbe und Bleistift, erstellt als Projektionsbildzeichnung für die Beobachtungen in Locarno-Monti auf einem eigenen Vordruck. Instrument: Zeiss-Coudé-Refraktor mit 15 cm Öffnung und 225 cm Brennweite. Bildqualität (SIDC): 3.
Die Beobachtungen zu Einzelflecken und zu Sonnenfleckengruppen wurden auf dem Vordruck eingetragen. Zusätzlich sind zuhanden der späteren statistischen Verarbeitung die Angaben für die Bestimmung der Fleckenrelativzahl notiert.
Beobachtungstätigkeit unter eigenständiger Leitung (Associazione Specola Solare Ticinese ASST)
Novel Transcriptional Regulatory Signals in the Adeno-Associated Virus Terminal Repeat A/D Junction Element
Creators: name: Samulski, R. J.
name: McCown, T. J.
name: Haberman, R. P.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2000

description: Adeno-associated virus (AAV) type 2 vectors transfer stable, long-term gene expression to diverse cell types in vivo. Many gene therapy applications require the control of long-term transgene expression, and AAV vectors, similar to other gene transfer systems, are being evaluated for delivery of regulated gene expression cassettes. Previously, we (R. P. Haberman, T. J. McCown, and R. J. Samulski, Gene Ther. 5:1604–1611, 1998) demonstrated the use of the tetracycline-responsive system for long-term regulated expression in rat brains. In that study, we also observed residual expression in the “off” state both in vitro and in vivo, suggesting that the human cytomegalovirus (CMV) major immediate-early minimal promoter or other cis-acting elements (AAV terminal repeats [TR]) were contributing to this activity. In the present study, we identify that the AAV TR, minus the tetracycline-responsive minimal CMV promoter, will initiate mRNA expression from vector templates. Using deletion analysis and specific PCR-derived TR reporter gene templates, we mapped this activity to a 37-nucleotide stretch in the A/D elements of the TR. Although the mRNA derived from the TR is generated from a non-TATA box element, the use of mutant templates failed to identify function of canonical initiator sequences as previously described. Finally, we demonstrated the presence of green fluorescent protein expression both in vitro and in vivo in brain by using recombinant virus carrying only the TR element. Since the AAV terminal repeat is a necessary component of all recombinant AAV vectors, this TR transcriptional activity may interfere with all regulated expression cassettes and may be a problem in the development of novel TR split gene vectors currently being considered for genes too large to be packaged.
Role of Dendritic Cell Targeting in Venezuelan Equine Encephalitis Virus Pathogenesis
Creators: name: MacDonald, G. H.
name: Johnston, R. E.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2000

description: The initial steps of Venezuelan equine encephalitis virus (VEE) spread from inoculation in the skin to the draining lymph node have been characterized. By using green fluorescent protein and immunocytochemistry, dendritic cells in the draining lymph node were determined to be the primary target of VEE infection in the first 48 h following inoculation. VEE viral replicon particles, which can undergo only one round of infection, identified Langerhans cells to be the initial set of cells infected by VEE directly following inoculation. These cells are resident dendritic cells in the skin, which migrate to the draining lymph node following activation. A point mutation in the E2 glycoprotein gene of VEE that renders the virus avirulent and compromises its ability to spread beyond the draining lymph blocked the appearance of virally infected dendritic cells in the lymph node in vivo. A second-site suppressor mutation that restores viral spread to lymphoid tissues and partially restore virulence likewise restored the ability of VEE to infect dendritic cells in vivo.
title: Abgelenkt durchs Internet und wieder nichts über Politik gelernt
Creators: name: Stjepanovic, Jelena
Year: 2022

description: Diese Arbeit untersucht die ablenkende Wirkung von nicht politischen Inhalten auf die Wissenswahrnehmung der politischen Beiträge und der politischen Partizipation auf Instagram. In einem 2x2 online Experiment (N=200) wurden manipulierte Instagram Feeds erstellt. Als ablenkende Inhalte wurden nicht politische Posts erstellt, welche durch die Nennung des Wohnorts der TeilnehmerInnen eine hohe Relevanz für diese hatten und humorvolle Posts, welche eingebettet zwischen den politischen Posts waren. Die Ergebnisse dieser Studie zeigen, dass relevante, ablenkende Posts, eine negative Wirkung und humorvolle Posts nur eine leicht negative Wirkung auf die politische Wissensaufnahme haben. Allerdings haben relevant ablenkende Posts keine negative Wirkung und humorvolle Posts haben sogar eine leicht positive Wirkung auf die politische Partizipation.
Sonnenfleckenzeichnung Nr. 242.
Zeichnung von Flecken und Fackelflächen zur Ermittlung der Sonnenaktivität am 12.11.2012, 09:00, erstellt von Sergio Cortesi
Creators: name: Observatorium Specola Solare Ticinese Locarno (1957 bis 1980 Teil der Eidgenössischen Sternwarte der ETH Zürich)
Publisher: name: [s.n.]
Year: 2012

description: Bleistiftzeichnung mit schwarzer Farbe und Bleistift, erstellt als Projektionsbildzeichnung für die Beobachtungen in Locarno-Monti auf einem eigenen Vordruck. Instrument: Zeiss-Coudé-Refraktor mit 15 cm Öffnung und 225 cm Brennweite. Bildqualität (SIDC): 3.
Die Beobachtungen zu Einzelflecken und zu Sonnenfleckengruppen wurden auf dem Vordruck eingetragen. Zusätzlich sind zuhanden der späteren statistischen Verarbeitung die Angaben für die Bestimmung der Fleckenrelativzahl notiert.
Beobachtungstätigkeit unter eigenständiger Leitung (Associazione Specola Solare Ticinese ASST)
Quasi-Monte Carlo point sets with small $t$-values and WAFOM
Creators: name: Harase, Shin
Publisher: name: arXiv
Year: 2014
Subjects: subject: Numerical Analysis (math.NA)
subject: FOS: Mathematics
subject: 65C05, 65D30

description: The $t$-value of a $(t, m, s)$-net is an important criterion of point sets for quasi-Monte Carlo integration, and many point sets are constructed in terms of the $t$-values, as this leads to small integration error bounds. Recently, Matsumoto, Saito, and Matoba proposed the Walsh figure of merit (WAFOM) as a quickly computable criterion of point sets that ensures higher order convergence for function classes of very high smoothness. In this paper, we consider a search algorithm for point sets whose $t$-value and WAFOM are both small, so as to be effective for a wider range of function classes. For this, we fix digital $(t, m, s)$-nets with small $t$-values (e.g., Sobol' or Niederreiter--Xing nets) in advance, apply random linear scrambling, and select scrambled digital $(t, m, s)$-nets in terms of WAFOM. Experiments show that the resulting point sets improve the rates of convergence for smooth functions and are robust for non-smooth functions.
description: 17 pages
INTRAMAMMARY ADMINISTRATION OF OXYGEN-OZONE MIX IN OIL VEHICLE BLENDED PLATELET CONCENTRATE AS AN UNCONVENTIONAL THERAPY IN BOVINE MASTITIS / FIRST CLINICAL APPLICATION
Creators: name: BIGNOTTI, ANDREA
Publisher: name: Universit� degli Studi di Milano
Year: 2015

description: Biological systems show a high level of complexity. For years single-target drugs have been successful and many pathologies have been considered cured and eradicated. As a matter of fact, the biological complex ecosystem was using the single-target chemical drugs information to evolve and find its own shortcuts. This is the case of antibiotic resistance. Planktonic bacteria evolve generating biofilm communities that are able to gambol antibiotic aggression and possibly evade the host immune system mechanisms. Synergistic solutions are needed to reactivate a virtuous balance in host?s favor: these solutions must be able to cooperate and eventually boost the host immune system, which has a naturally-embedded synergistic modus operandi; they must produce a reduced number of inconvenient metabolites, and they should exert a multifunctional activity. Antimicrobial peptides seem to convey the features required as they deploy a multifunctional antimicrobial set of measures, complying with the host immune system and offering regenerative effects on tissues. On the other end, ozone has been investigated for its bactericidal capacity and for its potential to reactivate depressed immune systems. As this experimental study accounts for, the administration to bovine mastitis of a blending of platelet concentrate, rich in antimicrobial peptides, with oxygen/ozone mix in oil vehicle was examined. Bovine mastitis is a public health issue and its control is fundamental to avoid serious consequences. Prevention should be the first approach to the problem: new predictive protocols and containment means need to be deployed and shared. However feasible effective treatments need to be available. The results of this clinical application point to a promising direction of investigation. In comparison with other therapies based on either a single component or a control group with antibiotic, the blending of platelet concentrate with oxygen/ ozone mix in oil vehicle accomplished the best outcomes in resolving the mastitic occurrence, downgrading and fixing the symptoms, and enhancing animal general conditions. Conceivable explanations for its efficacy have been suggested. Further and deeper research is required.
Sonnenfleckenzeichnung Nr. 240.
Zeichnung von Flecken und Fackelflächen zur Ermittlung der Sonnenaktivität am 08.11.2012, 08:00, erstellt von Marco Cagnotti
Creators: name: Observatorium Specola Solare Ticinese Locarno (1957 bis 1980 Teil der Eidgenössischen Sternwarte der ETH Zürich)
Publisher: name: [s.n.]
Year: 2012

description: Bleistiftzeichnung mit schwarzer Farbe und Bleistift, erstellt als Projektionsbildzeichnung für die Beobachtungen in Locarno-Monti auf einem eigenen Vordruck. Instrument: Zeiss-Coudé-Refraktor mit 15 cm Öffnung und 225 cm Brennweite. Bildqualität (SIDC): 3.
Die Beobachtungen zu Einzelflecken und zu Sonnenfleckengruppen wurden auf dem Vordruck eingetragen. Zusätzlich sind zuhanden der späteren statistischen Verarbeitung die Angaben für die Bestimmung der Fleckenrelativzahl notiert.
Beobachtungstätigkeit unter eigenständiger Leitung (Associazione Specola Solare Ticinese ASST)
Sindbis-Group Alphavirus Replication in Periosteum and Endosteum of Long Bones in Adult Mice
Creators: name: Simpson, D. A.
name: Heise, M. T.
name: Johnston, R. E.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2000

description: Several alphaviruses, including the Sindbis-group viruses, Ross River virus, O'nyong-nyong virus, and Chikungunya virus, are associated with outbreaks of acute and persistent arthralgia and arthritis in humans. Mechanisms underlying alphavirus-induced arthralgia and arthritis are not clearly understood, though direct viral replication within or around the affected joints is thought to contribute to disease. S.A.AR86 is a Sindbis-group alphavirus closely related to the arthralgia-associated Ockelbo and GirdwoodS.A viruses. Following inoculation with S.A.AR86 derived from a molecular clone, infectious virus was isolated from bone and joint tissue 1 to 6 days postinfection. Studies using either in situ hybridization or S.A.AR86-derived double promoter viruses and replicons expressing green fluorescent protein localized sites of viral replication to the periosteum, tendons, and endosteum within the epiphyses of the long bones adjacent to articular joints. These results demonstrate that alphaviruses associated with arthralgia in humans replicate within bone-associated connective tissue adjacent to articular joints in an adult mouse model.
Witten Index and Wall Crossing
Creators: name: Hori, Kentaro
name: Kim, Heeyeon
name: Yi, Piljin
Publisher: name: arXiv
Year: 2014
Subjects: subject: High Energy Physics - Theory (hep-th)
subject: FOS: Physical sciences

description: We compute the Witten index of one-dimensional gauged linear sigma models with at least ${\mathcal N}=2$ supersymmetry. In the phase where the gauge group is broken to a finite group, the index is expressed as a certain residue integral. It is subject to a change as the Fayet-Iliopoulos parameter is varied through the phase boundaries. The wall crossing formula is expressed as an integral at infinity of the Coulomb branch. The result is applied to many examples, including quiver quantum mechanics that is relevant for BPS states in $d=4$ ${\mathcal N}=2$ theories.
description: 123 pages, v3: the discussion on the smooth transition (Section 4.4) is improved, more minor corrections made; v2: references added, minor corrections made
Heterologous Gene Expression from Transmissible Gastroenteritis Virus Replicon Particles
Creators: name: Yount, B.
name: Baric, R. S.
name: Curtis, K. M.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2002

description: We have recently isolated a transmissible gastroenteritis virus (TGEV) infectious construct designated TGEV 1000 (B. Yount, K. M. Curtis, and R. S. Baric, J. Virol. 74:10600–10611, 2000). Using this construct, a recombinant TGEV was constructed that replaced open reading frame (ORF) 3A with a heterologous gene encoding green fluorescent protein (GFP). Following transfection of baby hamster kidney (BHK) cells, a recombinant TGEV (TGEV-GFP2) was isolated that replicated efficiently and expressed GFP. Replicon constructs were constructed that lacked either the ORF 3B and E genes or the ORF 3B, E, and M genes [TGEV-Rep(AvrII) and TGEV-Rep(EcoNI), respectively]. As the E and M proteins are essential for TGEV virion budding, these replicon RNAs should replicate but not result in the production of infectious virus. Following cotransfection of BHK cells with the replicon RNAs carrying gfp, GFP expression was evident by fluorescent microscopy and leader-containing transcripts carrying gfp were detected by reverse transcription-PCR (RT-PCR). Subsequent passage of cell culture supernatants onto permissive swine testicular (ST) cells did not result in the virus, GFP expression, or the presence of leader-containing subgenomic transcripts, demonstrating the single-hit nature of the TGEV replicon RNAs. To prepare a packaging system to assemble TGEV replicon particles (TGEV VRP), the TGEV E gene was cloned into a Venezuelan equine encephalitis (VEE) replicon expression vector and VEE replicon particles encoding the TGEV E protein were isolated [VEE-TGEV(E)]. BHK cells were either cotransfected with TGEV-Rep(AvrII) (E gene deletion) and VEE-TGEV(E) RNA transcripts or transfected with TGEV-Rep(AvrII) RNA transcripts and subsequently infected with VEE VRPs carrying the TGEV E gene. In both cases, GFP expression and leader-containing GFP transcripts were detected in transfected cells. Cell culture supernatants, collected ∼36 h posttransfection, were passed onto fresh ST cells where GFP expression was evident ∼18 h postinfection. Leader-containing GFP transcripts containing the ORF 3B and E gene deletions were detected by RT-PCR. Recombinant TGEV was not released from these cultures. Under identical conditions, TGEV-GFP2 spread throughout ST cell cultures, expressed GFP, and formed viral plaques. The development of infectious TGEV replicon particles should assist studies of TGEV replication and assembly as well as facilitate the production of novel swine candidate vaccines.
title: Frauen(stimmen) im Radio
Creators: name: Jaklin, Julia Maria
Year: 2022

description: In dieser Arbeit soll das von Audioquellen - sowie deren Metadaten - aus dem Bereich des Rundfunks eröffnete Spannungsverhältnis zwischen dem, was gesagt wird, und der Art und Weise, wie es gesagt wird, analysiert und insbesondere besonders die Zusammenhänge und Bedeutungen im Sinne einer Audio-basierten Geschichtsforschung gezeigt werden. Im Rahmen einer Fallstudie wurde die ORF-Radiosendung Von Tag zu Tag, eine Live-Radio- Interviewsendung, und eine ihrer regelmäßigen Moderatorinnen, Ilse Oberhofer, näher betrachtet. Ilse Oberhofer war eine der ersten Frauen, die in den frühen 1970er Jahren Nachrichtensendungen im österreichischen Radio moderierten und sich in Radiosendungen mit feministischen Themen befassten. Daher wird in dieser Arbeit auch versucht zu erörtern, wie die Karriere einer Frau und ihre Aktivitäten zur Sensibilisierung für Frauenfragen im Medium Radio dargestellt werden.
description: This thesis aims to discuss a case of biographical research taken from the field of radio broadcasting in Austria, using historic audio sources and available metadata. A speech analysis of these audio samples was conducted as not only the content of the spoken text transmits information, but also the quality of the voice, e.g. its tone, articulation, pauses, and so on. There is a tension between what is said and how it is said, and the analysis of this tension is only made possible by the availability of the audio sources. To illustrate my arguments and at the same time reflect upon possible approaches to voice analysis, this thesis aims to take a closer look at the ORF radio program Von Tag zu Tag, a live radio interview show, and one of its regular presenters, Ilse Oberhofer. Oberhofer was among the first women to present news broadcasts on Austrian radio in the early 1970s. Therefore, this thesis also trys to understand how a woman’s career and her activities in raising awareness of women’s issues is presented via the medium in which both her professional development and its social effects are documented.
Transcriptional Regulation of the K1 Gene Product of Kaposi's Sarcoma-Associated Herpesvirus
Creators: name: Bowser, B. S.
name: DeWire, S. M.
name: Damania, B.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2002

description: The K1 protein of Kaposi's sarcoma-associated herpesvirus (KSHV) has been shown to be a transforming protein capable of inducing morphological changes and focus formation in rodent fibroblasts. K1 can activate B-cell receptor (BCR) signaling and upregulate activity of the NFAT and NF-κB transcription factors. In order to understand the regulation of K1 gene expression, we have analyzed sequences upstream of the K1 gene to identify the K1 promoter element. We have performed 5′ rapid amplification of cDNA ends as well as a nuclease protection assay to map the transcriptional start site of the KSHV K1 transcript. The K1 transcriptional start site lies 75 bp upstream of the translation start site. Sequences upstream of the K1 gene were characterized for their ability to activate a luciferase reporter gene in 293 epithelial cells, KSHV-negative B cells (BJAB), KSHV-positive B cells (BCBL-1), and KS tumor-derived endothelial cells (SLK-KS−). We found that a 125-bp sequence upstream of the K1 transcript start site was sufficient to fully activate the luciferase reporter gene in all cell types tested. In addition, the viral transcription factor KSHV Orf50/Rta was capable of further activating this promoter element in 293, BJAB, and BCBL-1 cells but not in SLK-KS− cells. Promoter constructs containing additional sequences upstream of the 125-bp element did not show further augmentation of transcription in the presence or absence of KSHV Orf50.
Sonnenfleckenzeichnung Nr. 258.
Zeichnung von Flecken und Fackelflächen zur Ermittlung der Sonnenaktivität am 04.12.2012, 11:30, erstellt von Sergio Cortesi
Creators: name: Observatorium Specola Solare Ticinese Locarno (1957 bis 1980 Teil der Eidgenössischen Sternwarte der ETH Zürich)
Publisher: name: [s.n.]
Year: 2012

description: Bleistiftzeichnung mit schwarzer Farbe und Bleistift, erstellt als Projektionsbildzeichnung für die Beobachtungen in Locarno-Monti auf einem eigenen Vordruck. Instrument: Zeiss-Coudé-Refraktor mit 15 cm Öffnung und 225 cm Brennweite. Bildqualität (SIDC): 3.
Die Beobachtungen zu Einzelflecken und zu Sonnenfleckengruppen wurden auf dem Vordruck eingetragen. Zusätzlich sind zuhanden der späteren statistischen Verarbeitung die Angaben für die Bestimmung der Fleckenrelativzahl notiert.
Beobachtungstätigkeit unter eigenständiger Leitung (Associazione Specola Solare Ticinese ASST)
De Novo Infection with Rhesus Monkey Rhadinovirus Leads to the Accumulation of Multiple Intranuclear Capsid Species during Lytic Replication but Favors the Release of Genome-Containing Virions
Creators: name: Damania, B.
name: O'Connor, C. M.
name: Kedes, D. H.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2003

description: Rhesus monkey rhadinovirus (RRV) is one of the closest phylogenetic relatives to the human pathogen Kaposi's sarcoma-associated herpesvirus (KSHV), yet it has the distinct experimental advantage of entering efficiently into lytic replication and growing to high titers in culture. RRV therefore holds promise as a potentially attractive model with which to study gammaherpesvirus structure and assembly. We have isolated RRV capsids, determined their molecular composition, and identified the genes encoding five of the main capsid structural proteins. Our data indicate that, as with other herpesviruses, lytic infection with RRV leads to the synthesis of three distinct intranuclear capsid species. However, in contrast to the inefficiency of KSHV maturation following reactivation from latently infected B-cell lines (K. Nealon, W. W. Newcomb, T. R. Pray, C. S. Craik, J. C. Brown, and D. H. Kedes, J. Virol. 75:2866-2878, 2001), de novo infection of immortalized rhesus fibroblasts with RRV results in the release of high levels of infectious virions with genome-containing C capsids at their center. Together, our findings argue for the use of RRV as a powerful model with which to study the structure and assembly of gammaherpesviruses and, specifically, the human rhadinovirus,KSHV.
Sonnenfleckenzeichnung Nr. 238.
Zeichnung von Flecken und Fackelflächen zur Ermittlung der Sonnenaktivität am 06.11.2012, 09:00, erstellt von Sergio Cortesi
Creators: name: Observatorium Specola Solare Ticinese Locarno (1957 bis 1980 Teil der Eidgenössischen Sternwarte der ETH Zürich)
Publisher: name: [s.n.]
Year: 2012

description: Bleistiftzeichnung mit schwarzer Farbe und Bleistift, erstellt als Projektionsbildzeichnung für die Beobachtungen in Locarno-Monti auf einem eigenen Vordruck. Instrument: Zeiss-Coudé-Refraktor mit 15 cm Öffnung und 225 cm Brennweite. Bildqualität (SIDC): 2.
Die Beobachtungen zu Einzelflecken und zu Sonnenfleckengruppen wurden auf dem Vordruck eingetragen. Zusätzlich sind zuhanden der späteren statistischen Verarbeitung die Angaben für die Bestimmung der Fleckenrelativzahl notiert.
Beobachtungstätigkeit unter eigenständiger Leitung (Associazione Specola Solare Ticinese ASST)
Sonnenfleckenzeichnung Nr. 260.
Zeichnung von Flecken und Fackelflächen zur Ermittlung der Sonnenaktivität am 06.12.2012, 08:45, erstellt von Sergio Cortesi
Creators: name: Observatorium Specola Solare Ticinese Locarno (1957 bis 1980 Teil der Eidgenössischen Sternwarte der ETH Zürich)
Publisher: name: [s.n.]
Year: 2012

description: Bleistiftzeichnung mit schwarzer Farbe und Bleistift, erstellt als Projektionsbildzeichnung für die Beobachtungen in Locarno-Monti auf einem eigenen Vordruck. Instrument: Zeiss-Coudé-Refraktor mit 15 cm Öffnung und 225 cm Brennweite. Bildqualität (SIDC): 2.
Die Beobachtungen zu Einzelflecken und zu Sonnenfleckengruppen wurden auf dem Vordruck eingetragen. Zusätzlich sind zuhanden der späteren statistischen Verarbeitung die Angaben für die Bestimmung der Fleckenrelativzahl notiert.
Beobachtungstätigkeit unter eigenständiger Leitung (Associazione Specola Solare Ticinese ASST)
Valentini Haussmanns ... Fragmenta, oder, Fuenffunddreissig noch uebrige neue weltliche teutsche Lieder, meisten Theils mit vier, wenig aber mit fünff Stimmen von derselben Art wie bissher in unterschiedlichen fuenff Theilen mit anmuthigen Texten ... in dises Opusculum gebracht und publicirt
Fragmenta, oder, Fuenffunddreissig noch uebrige neue weltliche teutsche Lieder ...
Fragmenta
Creators: name: Haussmann, Valentin
Publisher: name: durch Paulum Kauffmann
Year: 1602

description: Cantus, Altus (je [4], XXXVI, [4] S.), Tenor mit Quinta Vox ([4], XXXVI [i.e. 44], [4] S.); Basis ([4], XXXVI, [2] S.)
Neue liebliche Melodien, unter neue teutsche weltliche Texte, derer jeder einen besondern Namen anzeiget, mit vier Stimmen, dess mehrern Theils zum Tantze zugebrauchen
Neue liebliche Melodien Singst 4
Creators: name: Haussmann, Valentin
Publisher: name: durch Paulum Kauffmann
Year: 1602

description: gesetzt durch Valentinum Haussmann ...
description: "3. Auflage" -- RISM
description: Cantus, Altus, Basis (je [1], XXX, [1] S.); Tenor ([4], XXX, [2] S.)
Neue artige und liebliche Taentze, zum Theil mit Texten, dass man kan mit menschlicher Stimme zu Instrumenten singen, zum Theil ohne Text gesetzt und denen, welche sich etwas neues gelieben lassen, zu gefallen publiciert
Neue artige und liebliche Tänze
Creators: name: Haussmann, Valentin
Publisher: name: durch Paulum Kauffmann
Year: 1602

description: durch Valentinum Haussmann ...
description: "4. Auflage" -- RISM
description: Cantus, Altus, Tenor, Basis: je [5], XXI, [14] S.
Epstein-Barr Virus Latent Membrane Protein 2A Activates  -Catenin Signaling in Epithelial Cells
Creators: name: Raab-Traub, N.
name: Morrison, J. A.
name: Klingelhutz, A. J.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2003

description: The Epstein-Barr virus (EBV) latent membrane protein 2A (LMP2A) functions to maintain latency in EBV-infected B lymphocytes. Although LMP2A is nonessential for the immortalization of B lymphocytes by EBV, its expression in B lymphocytes prevents viral reactivation by blocking B-cell receptor activation and signaling. LMP2A also provides an antiapoptotic signal in transgenic mice that express LMP2A in B lymphocytes. LMP2A activates phosphatidylinositol 3-kinase (PI3K) and the serine/threonine kinase Akt in lymphocytes and epithelial cells. Here we show that EBV LMP2A activates the PI3K and β-catenin signaling pathways in telomerase-immortalized human foreskin keratinocytes (HFK). LMP2A activated Akt in a PI3K-dependent manner, and the downstream Akt targets glycogen synthase kinase 3β (GSK3β) and the Forkhead transcription factor FKHR were phosphorylated and inactivated in LMP2A-expressing HFK cells. GSK3β is a negative regulator of the Wnt signaling pathway, and inactivation of GSK3β by LMP2A signaling led to stabilization of β-catenin, the central oncoprotein of Wnt signaling. In LMP2A-expressing cells, β-catenin accumulated in the cytoplasm and translocated into the nucleus via a two-step mechanism. The cytoplasmic accumulation of β-catenin downstream of LMP2A was independent of PI3K signaling, whereas its nuclear translocation was dependent on PI3K signaling. In the nucleus, β-catenin activated a reporter responsive to T-cell factor, and this activation was augmented by LMP2A coexpression. The Wnt pathway is inappropriately activated in 90% of colon cancers and is dysregulated in several other cancers, and these data suggest that activation of this pathway by LMP2A may contribute to the generation of EBV-associated cancers.
Irruption of massive particle species during inflation
Creators: name: Fedderke, Michael A.
name: Kolb, Edward W.
name: Wyman, Mark
Publisher: name: arXiv
Year: 2014
Subjects: subject: Cosmology and Nongalactic Astrophysics (astro-ph.CO)
subject: High Energy Physics - Phenomenology (hep-ph)
subject: FOS: Physical sciences

description: All species of (non-conformally-coupled) particles are produced during inflation so long as their mass $M$ is not too much larger than $H$, the expansion rate during inflation. It has been shown that if a particle species that is normally massive ($M\gg H$) couples to the inflaton field in such a way that its mass vanishes, or at least becomes small ($M < H$), for a particular value of the inflaton field, then not only are such particles produced, but an irruption of that particle species can occur during inflation. In this paper we analyze creation of a massive particle species during inflation in a variety of settings, paying particular attention to models which realize such an irruptive production mechanism.
description: 36 pages, 11 figures. Published version
title: Das Erstellen von GeoGebra-Applets und ihre Anwendungsmöglichkeiten in der 9. Schulstufe AHS
Creators: name: Sergi, Laura
Year: 2022

description: Die vorliegende Masterarbeit beschäftigt sich mit der Fragestellung, wie GeoGebra-Applets in der 9. Schulstufe eingesetzt werden können, um die mathematischen Grundvorstellungen von Schüler*innen auszubauen und zu festigen und somit zu einem tieferen Verständnis beizutragen. Nach einer theoretischen Einführung zu Grundvorstellungen, den Vorteilen der Technologienutzung im Mathematikunterricht und der diesbezüglichen Situation in Österreich, soll anschließend insbesondere auf die Erstellung von GeoGebra-Applets eingegangen werden. Dafür wird vorerst die Software mit allen für Applets relevanten Funktionen beschrieben, da die Arbeit gleichzeitig auch anderen Lehrpersonen helfen soll, Applets zu erstellen und diese im Unterricht einzusetzen. Mit Bezug auf den Lehrplan der 5. Klasse AHS und die in dieser Schulstufe auszubildenden Grundkompetenzen werden anschließend solche Applets generiert und fachlich als auch didaktisch sowie hinsichtlich ihrer Einsatzmöglichkeiten analysiert.
On the material geometry of continuously defective corrugated graphene sheets
Creators: name: Trzesowski, Andrzej
Publisher: name: arXiv
Year: 2014
Subjects: subject: Mathematical Physics (math-ph)
subject: FOS: Physical sciences
subject: 53A35, 14J81, 58E30, 74M25, 74A50

description: Geometrical objects describing the material geometry of continuously defective graphene sheets are introduced and their compatibility conditions are formulated. Effective edge dislocations embedded in the Riemann-Cartan material space and defined by their scalar density and by local Burgers vectors, are considered. The case of secondary curvature-type defects created by this distribution of dislocations is analysed in terms of the material space. The variational geometry of the material space closely related with the existence of a characteristic length parameter is proposed. The formula which describes, in a reference temperature, the influence of dislocations on the material Riemannian metric, is given.
description: 38 pages. Keywords: non-Euclidean geometry, orthogonal space, variational geometry, Riemann-Cartan space, surface, Gauss curvature, vectorial torsion, material geometry, isothermal geometry, material space, configurational space, embedding, curvature-type defect, line defect, dislocation, disclination, Burgers vector, Frank vector, graphene, graphene sheet, corrugation
Small-Molecule Inhibition of Human Immunodeficiency Virus Type 1 Replication by Specific Targeting of the Final Step of Virion Maturation
Creators: name: Dismuke, D.
name: Lee, K.-H.
name: Yuan, X.
name: Zhou, J.
name: Lundquist, C.
name: Forshey, B. M.
name: Chen, C. H.
name: Aiken, C.
Publisher: name: The University of North Carolina at Chapel Hill University Libraries
Year: 2004

description: Despite the effectiveness of currently available human immunodeficiency virus type 1 (HIV-1) therapies, a continuing need exists for new drugs to treat HIV-1 infection. We investigated the mechanism by which 3-O-{3′,3′-dimethylsuccinyl}-betulinic acid (DSB) inhibits HIV-1 replication. DSB functions at a late stage of the virus life cycle but does not inhibit the HIV-1 protease in vitro or interfere with virus assembly or release. DSB specifically delays the cleavage of Gag between the capsid (CA) and p2, resulting in delayed formation of the mature viral core and reduced HIV-1 infectivity. Replication of simian immunodeficiency virus (SIV) was resistant to DSB; however, a chimeric SIV carrying CA-p2 sequences from HIV-1 was inhibited by the drug, indicating that susceptibility to DSB maps to the CA-p2 region of the HIV-1 Gag protein. A single point mutation at the CA-p2 cleavage site of HIV-1 conferred strong resistance to DSB, confirming the target of the drug. HIV-1 strains that are resistant to a variety of protease inhibitors were sensitive to DSB. These findings indicate that DSB specifically protects the CA-p2 cleavage site from processing by the viral protease during virion maturation, thereby revealing a novel mechanism for pharmacologic inhibition of HIV-1 replication.