cometadata/datacite-rtg-text-other-reclassification-records huggingface dataset:
Real-Time Forecasting With a Mixed-Frequency VAR
Creators: name: Schorfheide, Frank
name: Dongho Song
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: Inorganic Chemistry
subject: FOS: Chemical sciences
subject: Mathematics
subject: FOS: Mathematics
subject: Information and Computing Sciences
subject: Biological Sciences
subject: Ecology
subject: FOS: Biological sciences
subject: Biotechnology

description: This article develops a vector autoregression (VAR) for time series which are observed at mixed frequencies—quarterly and monthly. The model is cast in state-space form and estimated with Bayesian methods under a Minnesota-style prior. We show how to evaluate the marginal data density to implement a data-driven hyperparameter selection. Using a real-time dataset, we evaluate forecasts from the mixed-frequency VAR and compare them to standard quarterly frequency VAR and to forecasts from MIDAS regressions. We document the extent to which information that becomes available within the quarter improves the forecasts in real time. This article has online supplementary materials.
Biophysical characterization of alpha-synuclein and its controversial structure
Creators: name: Alderson, Reid
Publisher: name: figshare
Year: 2014
Subjects: Biophysics

description: A thorough review of biophysical and structural investigations of the intrinsically disordered protein alpha-synuclein. We address the controversial reports that claimed the native state of alpha-synuclein was a helical tetramer.
Supporting Researchers in Creating Data Management Plans
Creators: name: Netscher, Sebastian
name: Schwickerath, Anna
name: Mauer, Reiner
name: Perry, Anja
Publisher: name: Zenodo
Year: 2020

description: Researchers are increasingly encouraged by different stakeholders to make research processes
 as transparent as possible, to enable reproducible research results and to share their
 (research) data FAIR and open with others. At the same time, the concrete requirements -
 often including the development of a data management plan - vary to a great extent between
 the different funding bodies, journals and research organizations. Likewise, there is often little
 concrete indication on how such requirements should be implemented or what is best practice
 to do so. Based on the work of Science Europe, we aim to define standardized, public and
 referenceable domain data protocols for educational research, serving as a ‘model’ data
 management plan for the corresponding community. Such domain data protocols support
 researchers in processing high quality data, based on the idea of replicable research, FAIR data
 and Open Science. Taking core standards and best-practices guidelines as well as legal and ethical issues into
 account, domain data protocols describe relevant activities to sample, clean, document and
 manage data appropriately, depending on the concrete type of data as well as on the method
 of sampling employed in the respective research project. Data, processed on the basis of
 domain data protocols are prepared for data archiving in a research data center, a (trusted)
 repository or data archive and thus for sharing with other researchers, both for the purpose of
 replication and for the re-use in new research contexts. Moreover, predefined domain data protocols can be used to support researchers in preparing
 project proposals when applying for funding. They aim at increasing researchers’ awareness of
 relevant tasks to undertake in the context of managing data and simplify budgeting of such
 activities in proposals. From the funder’s perspective, data protocols reduce the costs of
 reviewing funding applications as well as (periodical) reports on data management by
 suggesting and aiming at implementing standardized procedures. In sum, developing domain data protocols for educational research contributes to efforts
 undertaken in making data sharable, open and FAIR. In addition, the domain-specific protocols
 can be seen as prototypes for other research disciplines.
title: Managing riverscapes and flow regimes for biocultural diversity
Publisher: name: LE STUDIUM Loire Valley Institute for Advanced Studies
Year: 2021

description: For millennia, people have been part of nature in riverscapes, coevolving with the living river systems that are the hearts of these environments.  As well as being biologically diverse and resilient ecosystems, the world’s rivers have been paths for the evolution and adaptation of the cultural identity, material and spiritual wellbeing, and security of human civilizations.  River flows continue to connect people, places, and wildlife today, inspiring and sustaining a myriad beliefs, values, services, and lifeways.
Nucleotide-dependent interactions within a specialized Hsp70/Hsp40 complex involved in Fe-S cluster biogenesis
Creators: name: Alderson, Reid
Publisher: name: figshare
Year: 2014
Subjects: Structural Biology
Biochemistry

description: We used solution NMR spectroscopy to identify a sepcialized Hsp70's binding site on its Hsp40 co-chaperone. Only in the presence of ATP does Hsp70 interact with the J-domain of Hsp40, including its highly conserved His-Pro-Asp (HPD) motif.
Towards a Blueprint for Integrated Ocean Science
Creators: name: Nordlund, Lina Mtwana
name: Palacz, Artur Piotr
Publisher: name: Zenodo
Year: 2024

description: How do we integrate and collaborate across sectors and nations for a better understanding of the ocean and its marine life? In this poster presented at the 2024 UN Ocean Decade Conference in Barcelona, we introduce the concept of the Blueprint for Integrated Ocean Science which is being developed under the EU BioEcoOcean Project. The Blueprint is being designed as a support tool (guiding document) which will pose complex, "catch-all" questions, encouraging collaboration and holistic thinking across diverse environments and sectors. We invite participants from around the world who are interested in contributing to the co-creation of the Blueprint, and who are involved in any part of the ocean observing value chain: whether you are reviewing ocean data, planning observations, collecting, managing and reporting data, or developing data synthesis and modeling products, or work with policy or management.
Catalyzing the Open Science Transformation by an Institutional Strategy
Creators: name: Schirrwagen, Jochen
name: Wiljes, Cord
name: Pietsch, Christian
name: Hachmeister, Nils
name: Czerniak, Andreas
Publisher: name: Zenodo
Year: 2020
Subjects: Service Infrastructure
Policy
Training and Support
Working Group
Community Building

description: We understand the digital transformation of science as a socio-technical process in which digital tools
 and infrastructure go hand in hand with a (social) opening of essential phases of publicly funded
 research and teaching, taking into account subject-specific cultures. One can mandate this process from
 the top down, but in our experience a bottom up approach by researchers for researchers with the
 support of central facilities on the campus such as computing centre and library is more promising for
 the practice of an open scientific culture. In our opinion, the home institutions of the researchers have to play an important role in this
 transformative process. Policies on Open Access, on the management of research data and an Open Science Manifesto also
 provide the institutional framework at our institution. But this transformation process has only gained momentum with the establishment of an Open Access
 Task Force, now renamed into the Open Science Task Force, which brings together active members of
 various faculties and university institutions for almost ten years. This think tank meets regularly every
 two weeks and develops new ideas to support Open Science transformation up to its implementation in
 research projects. This group is an important driving force for the entire university which has to provide an infrastructure
 of services and tools for data publishing integrated seamlessly in the research process, efficient support
 to minimize the additional workload for researchers, policy and communication to raise awareness, and
 incentives to motivate data publishing. Since then, our university has consistently expanded its activities to include Open Science. Services for
 researchers include an institutional repository for publishing data, a GitLab installation that can
 perform automated data quality checks, and virtual research environments designed specifically for
 research projects. Particular attention was paid to integrating data management and open science into
 student education. A full-semester seminar has been offered for seven consecutive years and is included
 as an elective module in several degree programs. This is complemented by sharing of successful
 solutions with other universities and the adoption of promising approaches by others. We plan to
 present our overall approach to Open Science and the solutions developed so far, all of which are ready
 for reuse.
Real-Time Forecasting with a Mixed-Frequency VAR
Creators: name: Schorfheide, Frank
name: Dongho Song
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: 110309 Infectious Diseases
subject: FOS: Health sciences
subject: Inorganic Chemistry
subject: FOS: Chemical sciences
subject: Mathematics
subject: FOS: Mathematics
subject: Information and Computing Sciences
subject: Biological Sciences
subject: Ecology
subject: FOS: Biological sciences
subject: Biotechnology

description: This paper develops a vector autoregression (VAR) for time series which are observed at mixed frequencies – quarterly and monthly. The model is cast in state-space form and estimated with Bayesian methods under a Minnesota-style prior. We show how to evaluate the marginal data density to implement a data-driven hyperparameter selection. Using a real-time data set, we evaluate forecasts from the mixed-frequency VAR and compare them to standard quarterly-frequency VAR and to forecasts from MIDAS regressions. We document the extent to which information that becomes available within the quarter improves the forecasts in real time.
DIGITAL KILLED THE LABELLING STAR: APPROACHING THE TERRITORY-MUSEUM WITH MOBILE TECHNOLOGY
Creators: name: Hobma, Heather
Publisher: name: figshare
Year: 2015
Subjects: Museology
History
Digital Humanities

description: Visitors to historical locations in the United Kingdom step into what is, in essence, a virtual world of history and cultural heritage. Whether they realise this or not—and whether their experience at these sites is enriched by the recognition of the connections to other related objects and sites in the region—is determined in large part by the quality and coherence of the interpretative information available to them. This thesis focuses on how this interpretation is currently carried out at three sites, Hoddom, Ruthwell, and Bewcastle, and the possibilities for improvement based on the application of different media and technologies.
Sunlight is the best disinfectant: retractions and the role of Open Access
Creators: name: Schmitz, Jasmin
Publisher: name: Zenodo
Year: 2020
Subjects: Open Access
Retractions
Good Scientific Practice

description: Retractions can be considered as part of the “self-purification” of science: If a journal article contains serious errors or violations of good scientific practices the publication should be retracted. Depending on the type of defectiveness, notices about problematic journal articles can be published as “correction” (for minor flaws), “expression of concern” (for initial suspicion raised by the editorial board) or proper “retraction” (for misconduct) (Sox & Drummond, doi: 10.7326/0003-4819-144-8-200604180-00123). Especially in medicine and other health sciences the detection of problematic publications and their retraction is essential in order to protect patients and public health. Studies show that there is a rise in the number of retractions (e.g. Li et al., doi: 10.2147/JMDH.S151745). Reasons might be an increase of awareness of editorial boards taking action much faster (Steen et al., doi: 10.1371/journal.pone.0068397) also shown in the fact that more and more journals give themselves a “retraction policy” (Resnik et al. doi: 10.3163/1536-5050.103.3.006). Open access as an essential part of open science movement is intended to raise the transparency of science as such. The overall question therefore is: Which role does open access play with regard to retractions? The poster will present results from an initial – kind of proof of concept – analysis of retractions collected from the “Retraction Watch Database” (http://retractiondatabase.org) for articles from the health sciences published in 2019. With the help of the SHERPA/RoMEO database (http://sherpa.ac.uk/romeo/index.php) journals are assigned to the categories open access or hybrid. There will be a check on article level for publications from hybrid journals to see if they are published via an open access option. The analysis will focus on the questions: What is the share of open access among retracted articles? Is there a difference in the justification for retractions between open access and paywall articles and in the time lag from publication to retraction? For those publications behind a paywall there will be checks in Unpaywall (https://unpaywall.org/) and Open Access Button (https://openaccessbutton.org/) to identify open access versions to include this aspect into the analysis as well. The results will serve as a basis for a discussion whether “openness” on the level of publication has an effect on the self-purification process of science.
Internet of Intelligences Can Be a Platform for Risk Analysis and Management
Creators: name: Chongfu Huang
Publisher: name: Taylor & Francis
Year: 2015
Subjects: Science Policy
Biological Sciences

description: ABSTRACTIn this article, we clarify the concept of the Internet of Intelligences (IOI) to be a platform for risk analysis. An IOI is a triple consisting of a set of agents, a network, and a model. A process of processing homological information is a layer. An IOI with more than one layer is a Multiple Internet of Intelligences (M-IOI). If agents in an M-IOI not only provide information in response to a question asked by a customer but also review information from other agents and summarize it, we refer to this as a summarizing M-IOI. In this article, a simplest M-IOI is used to answer a question issued by an insurance company considering selling policies in Wenzhou, China, where the level of typhoon risk is high. We employ the normal diffusion model to process the summaries and give an adequate answer.
A General Model of Random Variation
Creators: name: Shore, Haim
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Plant Biology
subject: FOS: Biological sciences
subject: 111714 Mental Health
subject: FOS: Health sciences
subject: Cancer
subject: Mathematics
subject: FOS: Mathematics
subject: Biological Sciences
subject: Ecology
subject: Earth and Environmental Sciences
subject: Genetics
subject: Biophysics

description: A statistical distribution of a random variable is uniquely represented by its normal-based quantile function. For a symmetrical distribution it is S-shaped (for negative kurtosis) and inverted S-shaped (otherwise). As skewness departs from zero, the quantile function gradually transforms into a monotone convex function (positive skewness) or concave function (otherwise). Recently, a new general modeling platform has been introduced, response modeling methodology, which delivers good representation to monotone convex relationships due to its unique “continuous monotone convexity” property. In this article, this property is exploited to model the normal-based quantile function, and explored using a set of 27 distributions.
Is plastoquinone10-ox an antioxidant marker of red wines?
Creators: name: Gvozdjákova, Anna
name: Kucharská, J.
name: Duršin, P.
name: Minárik, E.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Research Note Antioxidant activities of 20 different types of Slovak wines were examined. For the first time evidence of plastoquinone10-ox (PQ10-ox)) in red wines was documented. Twice higher levels of alpha-tocopherol (vitamin E) were determined in red wine varieties in comparison to white wine varieties. Enzymatic actitivities of superoxidedismutase (SOD) in all red wine varieties were higher compared to white wines; in some white wines they were not detectable. The beneficial effects of antioxidants are discussed, especially the role of coenzyme Q10 (analogue of plant PQ10-ox), vitamin E and SOD in preventing cardiovascular diseases. It is supposed that PQ10-ox could be an antioxidant marker of red wines. To prove the protective effect of a moderate consumption of red wine in human cardiology further studies are required.
description: VITIS - Journal of Grapevine Research, Vol. 35 No. 2 (1996): Vitis
title: La Fabrique des inégalités sociales de santé
Publisher: name: Institut Fédératif d’Études et de Recherches Interdisciplinaires Santé Société
Year: 2021

description: Ce symposium proposé en 2020 en visioconférence s'intéresse à la fabrique des inégalités sociales de santé, du point de vue des disciplines de sciences humaines et sociales.
title: Quel avenir pour les plantations forestières ?
Publisher: name: Réseau Thématique de Recherche Milieux et Diversité
Year: 2019

description: Deuxième séminaire du projet Reforeste   18 novembre 2019     Après un premier séminaire consacré aux plantations forestières, organisé en mai 2019 (supports et vidéos des interventions consultables : https://reforeste.univtours.fr/ressources/), l’équipe du projet Reforesté a organisé ce deuxième qui visait plus particulièrement à interroger l’avenir de ces formations végétales, notamment en considérant le changement climatique. Ce séminaire s’inscrit dans le programme d’animation du Réseau thématique régional MiDi (Milieux et Diversité).   À cette occasion, nous avons eu le plaisir d’accueillir : Jean-François Dhôte (UMR 0588 INRA-ONF BioForA, France) Tatenda Mapeto (Nelson Mandela University, South Africa))
Internet of Intelligences Can Be a Platform for Risk Analysis and Management
Creators: name: Chongfu Huang
Publisher: name: Taylor & Francis
Year: 2015
Subjects: Science Policy
Biological Sciences

description: ABSTRACTIn this paper, we clarify the concept of the Internet of Intelligences (IOI) to be a platform for risk analysis. An IOI is a triple consisting of a set of agents, a network, and a model. A process of processing homological information is a layer. An IOI with more than one layer is a Multiple Internet of Intelligences (M-IOI). If agents in an M-IOI not only provide information in response to a question asked by a customer but also review information from other agents and summarize it, we refer to this as a summarizing M-IOI. In this paper, a simplest M-IOI is used to answer a question issued by an insurance company considering to sell policies in Wenzhou, China, where the level of typhoon risk is high. We employ the normal diffusion model to process the summaries and give an adequate answer.
Characterization of ethanol tolerance of yeasts using a calorimetric technique
Creators: name: Antoce, A.
name: Takahashi, K.
name: Namolosanu, I.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Research Note
description: VITIS - Journal of Grapevine Research, Vol. 35 No. 2 (1996): Vitis
A General Model Of Random Variation*
Creators: name: Shore, Haim
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Plant Biology
subject: FOS: Biological sciences
subject: 111714 Mental Health
subject: FOS: Health sciences
subject: Cancer
subject: Mathematics
subject: FOS: Mathematics
subject: Biological Sciences
subject: Ecology
subject: Earth and Environmental Sciences
subject: Genetics
subject: Biophysics

description: ABSTRACTA statistical distribution of a random variable is uniquely represented by its normal-based quantile function. For a symmetrical distribution it is S-shaped (for negative kurtosis) and inverted S-shaped (otherwise). As skewness departs from zero, the quantile function gradually transforms into a monotone convex function (positive skewness) or concave function (otherwise). Recently, a new general modeling platform has been introduced, Response Modeling Methodology, which delivers good representation to monotone convex relationships due to its unique “continuous monotone convexity” property. In this paper, this property is exploited to model the normal-based quantile function, and explored using a set of 27 distributions.
Importance of the vegetative stage for phytoplasma detection in yellow-diseased grapevines
Creators: name: Del Serrone, Paola
name: Barba, Marina
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Research Note The influence of the vegetative stage on the detection of phytoplasmas (=MLOs) in different grapevine tissues during six phenological stages is reported. From the beginning of the annual growth cycle of the plants it is possible to detect phytoplasmas in infected vines using purified DNA from leaves and shoots in slot blot hybridization. The hybridization signal is stronger in later stages reaching the highest intensity with leaf tissue at berry ripening. The detection is possible with cortical scrapings from mature canes. This opens an interesting possibility for massive screening of propagative material for certification purposes.
description: VITIS - Journal of Grapevine Research, Vol. 35 No. 2 (1996): Vitis
Some properties of a hitherto undescribed filamentous virus of the grapevine
Creators: name: Choueiri, E.
name: Boscia, D.
name: Digiaro, M.
name: Castellano, M. A.
name: Martelli, G. P.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: An apparently new, non mechanically-transmissible clostero-like virus, for which the name ''grapevine leafroll-associated virus 7('') (GLRaV-7) is proposed, was found in Albanian grapevine accessions. Virus particles were filamentous, had conspicuous cross banding and a length of 1500-1700 nm. Virions had coat protein subunits with an estimated M(r) of ca. 37 kDa and a ssRNA genome with size of ca. 19.5 kb as deduced from the estimate of dsRNA (ca. 19.5 kbp) extracted from grapevine tissues. A virus-specific antiserum was raised, which decorated virions at a dilution of 1:1000. This antiserum did not recognize particles of any of the six grapevine leafroll-associated clostero-like viruses (GLRaV-1 to -6) known to date, nor of grapevine trichovirus A (GVA) and B (GVB). Grapevine indicators graft-inoculated with material from accessions containing GLRaV-7 reacted with mild leafroll-like symptoms. In a survey in which 2226 vines from 30 different countries were examined by ELISA, GLRaV-7 was found in 141 plants from Albania, Greece, Hungary, Egypt, and Italy.
description: VITIS - Journal of Grapevine Research, Vol. 35 No. 2 (1996): Vitis
Aseptic dual culture of grape (Vitis spp.) and grape phylloxera (Daktulosphaira vitifoliae FITCH)
Creators: name: Forneck, Astrid
name: Walker, M. A.
name: Merkt, N.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: An aseptic dual culture of grape phylloxera (Daktulosphaira vitifoliae FITCH) and grape vine (Vitis spp.) was developed. This method permits continuous observation of phylloxera feeding and the whole plant response on a dynamic basis. The plant/parasite interaction of three testplants (V. vinifera L., var. Riesling, SO 4 (V. berlandieri PLANCH. X V. riparia L.) and V. riparia, var. Gloire de Montpellier) are demonstrated by observing post-infectious reactions of the host- and population dynamics of the parasite. Different stages of phylloxera could be observed including nymphs, winged phylloxera (alatae) and sexual male phylloxera. Several potential applications for this aseptic dual culture are demonstrated.
description: VITIS - Journal of Grapevine Research, Vol. 35 No. 2 (1996): Vitis
Linked Open Data - Toepassingen in de praktijk
Creators: name: Koster, Lukas
Publisher: name: figshare
Year: 2014
Subjects: subject: Library and Information Studies
subject: FOS: Media and communications
subject: Information Systems
subject: FOS: Computer and information sciences
subject: Distributed Computing
subject: Data Format
subject: Applied Computer Science

description: Article (in Dutch) published in Informatieprofessional magazine, 2014, issue 6. Verschenen in Informatieprofessional, jaargang 2014, nummer 6.
Detection of somaclonal variation in grapevine regenerants from protoplasts by RAPD-PCR
Creators: name: Schneider, Sabine
name: Reustle, G.
name: Zyprian, Eva
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Research Note
description: VITIS - Journal of Grapevine Research, Vol. 35 No. 2 (1996): Vitis
(Abstract) Measuring self-regulated learning in the workplace.
Creators: name: Fontana, Rosa Pia
name: Milligan, Colin
name: Littlejohn, Allison
name: Margaryan, Anoush
Publisher: name: figshare
Year: 2014
Subjects: Education

description: Abstract for a paper recently accepted for International Journal of Training and Development (to appear v19,1: 2015). Work from the WLBK project.
Stomatal patchiness of grapevine leaves. 2. Uncoordinated and coordinated stomatal movements
Creators: name: Düring, H.
name: Stoll, M.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: The dynamics of stomatal patchiness of grapevine leaves (var. Richter 110) were studied by in situ infiltration of water into the intercellular spaces (see: DÜRING and LOVEYS 1996). As infiltrations were shown not to affect stomatal conductance (g) a series of experiments was performed in which a leaf segment was infiltrated and photographed repeatedly. While stomata of some patches did not alter their apertures within a 90-minute experiment, others opened and closed their stomata more or less frequently leading to irregular fluctuations of patches with open, partly open and closed stomata. In contrast to this uncoordinated behavior coordinated, synchronous stomatal movements were recorded by gas exchange. Sinus wave-like stomatal oscillations with periods of 32-70 min and amplitudes of 38-95 mmol CO2 m-2 s-1 at constant ambient conditions were observed in a 12 h experiment. The stomatal oscillations were closely related to rhythmic alterations of the intercellular CO2 concentration (ci) and to the rate of CO2 assimilation (A). An increase of amplitudes of g was associated with a decrease of the carboxylation efficiency (A/ci) and the water use efficiency (A/g). It is concluded that uncoordinated, patchy fluctuations of stomatal apertures enable effective adaptation of single patches to changes of ambient stress factors.
description: VITIS - Journal of Grapevine Research, Vol. 35 No. 2 (1996): Vitis
Estimation de la capacité photoautotrophique de vitroplants de porte-greffe de vigne (var. Gravesac): mise au point d'un système de mesure de la photosynthèse nette de vitroplants
Creators: name: Lima Da Silva, A.
name: Hariscain, P.
name: Ollat, Nathalie
name: Doazan, J. P.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: La capacité photosynthétique de vitroplants de vigne (var. Gravesac) a été déterminée à l'aide d'un dispositif de mesures des échanges gazeux en circuit ouvert. L'activité photosynthétique et la respiration de la plante sont importantes en culture in vitro. La photosynthèse nette maximale est de 2 µmol m-2 s-1 a 500 µ11-1 de CO2. A 350 µ11-1 de CO2 la photosynthèse est de 1,6 µmol m-2 s-1. La respiration a l'obscurité est de 0,018 µmol s-1 g-1 de poids sec total. La quantité totale des sucres solubles dans le milieu de culture reste constante pendant les deux mois de culture. Le saccharose est totalement hydrolysé en glucose et en fructose. Les vitroplants de Gravesac ne prélèvent pas de sucres dans le milieu, ce qui confirme leur degré élevé d'autotrophie dans ces conditions de culture. La comparaison entre les échanges gazeux de vitroplants et des plantes cultivées en serre met en évidence une réduction de 80% de l'activité in vitro. Evaluation of the photoautotrophic ability of Vitis plantlets (rootstock var Gravesac): Set-up of a special open flow gas exchange system The photosynthetic ability of Vitis rootstock plantlets (var. Gravesac) was estimated in vitro with an open flow system specially designed for in vitro culture. CO2 response curves were established and respiration was also measured. The maximum net photosynthetic rate, reached at 500 µ11-1 of CO2, is 2 µmol m-2 s-1. At 350 µ11-1 CO2 the photosynthetic activity is 1.6 µmol m-2 s-1. The dark respiration rate is 0.018 µmol s-1 g-1 of total dry weight. The total amount of soluble carbohydrates in the medium remains the same whether plantlets were present or not. In the medium with plantlets, the sucrose is entirely hydrolysed in glucose and fructose within two months. No carbohydrate uptake from the medium was recorded indicating an autotrophic behaviour. Measurements of CO2 exchange were also performed at leaves of plants cultivated in a greenhouse. The photosynthetic activity of in vitro plants accounts only for 20% of the level of greenhouse-grown plants.
description: VITIS - Journal of Grapevine Research, Vol. 35 No. 2 (1996): Vitis
Methylated DNA changes associated with the initiation and maintenance of Vitis vinifera in vitro shoot and callus cultures: A possible mechanism for age-related changes
Creators: name: Harding, K.
name: Benson, Erica E.
name: Roubelakis-Angelakis, Kalliope A.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Tissue culture technologies are an important aspect of the genetic modification of grapevine (Vitis vinifera). The molecular basis of this phenomenon is not well understood, however, the extent of DNA methylation is recognised as a factor in the control of gene expression. This study explores the possibility that DNA methylation may have a role in grapevine culture responses. DNA methylation profiles were constructed for Vitis vinifera, cv. Sultanina, during plantlet micropropagation, callus induction and proliferation. Methylation of genomic DNA and ribosomal RNA genes (rDNA) was found in glasshouse-grown plants, micropropagated plantlets and callus cultures. An analysis of rDNA showed that glasshouse-grown plants had 74.6% of the recognition sequences for Hpa II methylated at the internal cytosine position CmCGG, whereas 7.7% of the recognition sequences appeared to be methylated at the external cytosine (mCmCGG). The rDNA profiles of micropropagated subcultures S0 (initial subcultures) and S4 (fourth subcultures), representing one year of in vitro growth, showed that the percentage of recognition sequences containing a methylated external cytosine increased from 7.7% in glasshouse-grown plants to 64.5% for S-0 and 72.5% for the S4 subculture. The implications of these findings for the in vitro manipulation of grapevine used in genetic modifications are discussed.
description: VITIS - Journal of Grapevine Research, Vol. 35 No. 2 (1996): Vitis
Extraction of DNA from eggs of grape phylloxera (Daktulosphaira vitifoliae FITCH) for use in RAPD testing
Creators: name: Lin, Hong
name: Walker, M. A.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Three different extraction methods for preparing DNA from single grape phylloxera (Daktulosphaira vitifoliae) eggs for use in PCR-RAPD assay were compared. Pulverization in a glass micro-grinder produced much more clear and consistent RAPD results than when the egg DNA was extracted with a Chelex lysis or a proteinase-K+ lysis. Multiple single egg extractions from the same parthenogenetic colony were done with all three methods; no RAPD assay differences were detected. This technique will allow evaluation of the roles population diversity, mutation rates, and reproductive mechanisms play in the Vitis/phylloxera interaction.
description: VITIS - Journal of Grapevine Research, Vol. 35 No. 2 (1996): Vitis
Phytochemical and antioxidant profiles of leaves from different Sorbus L. species
Creators: name: Raudonė, Lina
name: Raudonis, Raimondas
name: Gaivelytė, Kristina
name: Pukalskas, Audrius
name: Viškelis, Pranas
name: Venskutonis, Petras Rimantas
name: Valdimaras Janulis
Publisher: name: Taylor & Francis
Year: 2014
Subjects: subject: Ecology
subject: FOS: Biological sciences
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Cell Biology
subject: Biochemistry

description: Leaves of Sorbus L. have been used in various traditional medicine systems. Phenolic compounds determine the main pharmacological effects of Sorbus L. In this study, phytochemical and antioxidant profiles of Sorbus anglica, Sorbus aria, Sorbus arranensis, Sorbus aucuparia, Sorbus austriaca, Sorbus caucasica, Sorbus commixta, Sorbus discolor, Sorbus gracilis, Sorbus hostii, Sorbus semi-incisa and Sorbus tianschanica were determined. Twenty four constituents were identified in Sorbus L. species using ultra high performance liquid chromatography coupled to quadruple and time-of-flight mass spectrometers. Post-column FRAP assay identified compounds with reducing activity and revealed significantly greatest total antioxidant activity of 175.30 μmol TE/g DW, 169.20 μmol TE/g DW and 148.11 μmol TE/g DW in S. commixta, S. discolor and S. gracilis leaf samples, respectively, with neochlorogenic and chlorogenic acids being most significant contributors. Characteristic fingerprints of phytochemical and antioxidant profiles could be applied for the quality evaluation of various raw materials of Sorbus L. species.
Ethanolic extract of Ferula gummosa is cytotoxic against cancer cells by inducing apoptosis and cell cycle arrest
Creators: name: Gudarzi, Hoda
name: Salimi, Mona
name: Irian, Saeed
name: Amanzadeh, Amir
name: Hirsa Mostafapour Kandelous
name: Keyhan Azadmanesh
name: Salimi, Misha
Publisher: name: Taylor & Francis
Year: 2015
Subjects: subject: Hematology
subject: Science Policy
subject: Cancer
subject: Biological Sciences
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Biotechnology
subject: Molecular Biology
subject: Cell Biology
subject: Microbiology
subject: FOS: Biological sciences
subject: Biochemistry

description: Ferula gummosa Boiss. has medicinal applications in treating a wide range of diseases including cancer. The objective of this study was to evaluate the antiproliferative activities of the seed and gum extracts of F. gummosa as well as to study the effect of the potent extract on the induction of apoptosis and cell cycle arrest. Our results demonstrated that the ethanolic extract had the lowest IC50 value at 72 h (0.001 ± 1.2 mg/mL) in BHY cells. Moreover, flowcytometry and annexin-V analysis revealed that the ethanolic extract induced apoptosis and cell-cycle arrest in BHY cells at G1/S phase. In addition, colorimetric methods exhibited the highest amount of total phenolics and flavonoids in the aqueous and gum extracts (0.12 ± 0.037, 0.01 ± 2.51 mg/g of dry powder). Generally, the results obtained indicate that F. gummosa ethanol extract may contain effective compounds which can be used as a chemotherapeutic agent.
Stomatal patchiness of grapevine leaves .1. Estimation of non-uniform stomatal apertures by a new infiltration technique
Creators: name: Düring, H.
name: Stoll, M.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Non-uniform stomatal behaviour of vine leaves is associated with a heterobaric leaf structure. A microscopical analysis of cross sections of glasshouse- and in vitro-grown Silvaner leaves indicates single airspaces which are pneumatically isolated by vessels, bundle sheath extensions and the abaxial and adaxial epidermes. A pressure-regulated infiltration technique is presented by which the infiltration process and the infiltration capacity (percentage of the surface area of infiltrated airspaces) can be estimated and photographed using a light microscope. The average surface area of airspaces ranged from 0.10 mm2 (Regent) to 0.14 mm2 (Silvaner), the number of stomata per airspace from 35 (Regent) to 42 (Silvaner). The infiltration capacity of turgid leaves is shown to be negatively correlated with the surface tension of the infiltrated liquid and positively with stomatal conductance and with infiltration pressure, except for very low stomatal conductances (e.g. 12 mmol H2O m-2 s-1). The latter relationship follows a saturation curve confirming heterogenous stomatal aperture over the leaf blade. The distribution of stomatal apertures does not appear to be bimodal but to follow a bell-shaped curve. There is some evidence for the stomata of an airspace to behave heterogenously as well.
description: VITIS - Journal of Grapevine Research, Vol. 35 No. 2 (1996): Vitis
Measuring self-regulated learning in the workplace.
Creators: name: Fontana, Rosa Pia
name: Milligan, Colin
name: Littlejohn, Allison
name: Margaryan, Anoush
Publisher: name: figshare
Year: 2014
Subjects: Education

description: Abstract for a paper recently accepted for International Journal of Training and Development (to appear v19,1: 2015). Work from the WLBK project.
Molecular recognition studies by nanoESI mass spectrometry
Creators: name: Danquah, Bright Djan
Publisher: name: Universität Rostock
Year: 2019
Subjects: subject: 500 Natural sciences
subject: 610 Medical sciences Medicine

description: Analytical methods that characterize antibody-antigen interactions are certainly worth developing as they illuminate our understanding of molecular mechanisms of certain disease processes, and ultimately provide opportunities to improve existing treatment and management of such diseases by designing novel therapeutics. In this thesis, the challenges associated with the existing methods have been surpassed by developing simple but accurate nanoESI mass spectrometry methods which can be used to characterize antibody specificities as well as estimation of their binding affinities.
A new chromone from Hymenocallis littoralis Salisb. (Amaryllidaceae)
Creators: name: Anh, Dinh Thi Phuong
name: Duong, Tran Bach
name: Hoang, Vu Dinh
Publisher: name: Taylor & Francis
Year: 2014
Subjects: subject: Plant Biology
subject: FOS: Biological sciences
subject: Inorganic Chemistry
subject: FOS: Chemical sciences
subject: Biological Sciences
subject: Sociology
subject: FOS: Sociology
subject: Ecology
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Pharmacology
subject: Biochemistry

description: A new chromone, 5,7-dihydroxy-6,8-dimethoxy-2-methyl-4H-chromen-4-one (1), together with seven known compounds, 5,7-dihydroxy-6-methoxy-2-methyl-4H-chromen-4-one (pisonin B) (2), 5,7-dihydroxy-2-methyl-4H-chromen-4-one (noreugenin) (3), 5-hydroxy-7-methoxy-2-methyl-4H-chromen-4-one (eugenin) (4), (2S)-7,4′-dihydroxyflavan (5), 3′,7-dihydroxy-4′-methoxy-8-methylflavan (6), 3′,7-dihydroxy-4′-methoxyflavan (7) and 6,8-dimethyl-5,7,4′-trihydroxyflavanone (( − )-farrerol) (8), were isolated from Hymenocallis littoralis Salisb. (Amaryllidaceae) growing in Vietnam. Their structures were determined based on spectroscopic and physicochemical analyses.
Inheritance of seedlessness in grapevine (Vitis vinifera L.)
Creators: name: Bouquet, A.
name: Danglot, Y.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Despite considerable efforts made by breeders for over 70 years, inheritance of seedlessness in grapevine is not clearly defined. None of the numerous hypotheses proposed until now is satisfying, whether they are based on recessive or dominant genes. We measured precisely the phenotypic expression of the seeded/seedless character in a progeny obtained by crossing two partially seedless selections and using in ovulo and in vitro culture to rescue embryos. We propose the hypothesis that inheritance of seedlessness in grapevine is based on a complex system whereby the expression of three independently inherited recessive genes is controlled by a dominant regulator gene. This hypothesis was compared to other results published in the scientific literature and appeared coherent enough to be used as a theoretical basis for further work on seedlessness inheritance in grapevine. Attempts to explain the control of seedlessness involve interactions with endogenous gibberellins. The consequences of such a model for the development of breeding programs for seedless table grapes, and particularly for the use of molecular biology techniques, are discussed.
description: VITIS - Journal of Grapevine Research, Vol. 35 No. 1 (1996): Vitis
Recherche de gènes de résistance naturelle à deux viroses de la vigne: le court-noné et l'enroulement
Creators: name: Lahogue, Florence
name: Boulard, G.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Un grand nombre de vignes (734) représentant les différentes classes de la famille des Vitacées (Vitis américains et asiatiques, Vitis vinifera, hybrides interspécifiques) a été testé vis-à-vis de leur résistance naturelle aux virus du court-noué (GFLV et ArMV) èt de l'enroulement (GLRaV-1 et 3). L'accent a été mis sur les V. vinifera d'origine moyen-orientale du fait de la possible coévolution de la vigne et du GFLV dans ces pays. La méthode d'inoculation utilisée a été la greffe-bouture herbacée. Mais il s'est avéré que par cette technique, il était difficile de reproduire à l'identique les conditions de l'inoculation. En effet, toutes les variétés n'ont pas la même aptitude au greffage et l'état physiologique des plantes joue également un rôle important sur ce facteur. Ainsi, nous avons considéré qu'une variété était sensible si le virus avait été détecté dans au moins une de ses greffes . Ce qui a été le cas de toutes les variétés pour lesquelles le greffage avec un inoculum GFLV ou GLRaV a réussi. L'inoculation de l'ArMV est apparue plus difficile à obtenir, sans doute du fait que la vigne n'est pas un hôte principal de ce virus. Il semble qu'en fait cette méthode d'inoculation ne soit pas adaptée au but recherché. La pression d'inoculation est d'une part trop forte. D'autre part, le virus étant directement mis en contact avec les vaisseaux, la technique ne permet pas de repérer les résistances qui pourraient intervenir aux premiers stades d'une infection naturelle . Investigations on natural resistance genes for two grapevine viruses: The fanleaf degeneration and the leafroll disease A great number of grapevine varieties (734) belonging to the different classes of the Vitaceae family (American and Asian Vitis, Vitis vinifera, interspecific hybrids) was screened to identify sources of resistance to fanleaf degeneration (GFLV and ArMV) and to leafroll disease (GLRaV-1 and 3). Special attention was paid to Middle Eastern Vitis vinifera sources due to the possibility that GFLV and V. vinifera evolved together in the Middle East. Inoculation was by green grafting. As the inoculation conditions were difficult to control, we considered a variety to be susceptible if the virus was found in at least one graft. That was true for all varieties for which the graft with a GFLV or GLRaV inoculum had been successful. It appeared to be more difficult to inoculate ArMV, possibly because the grapevine is not the principal host of this virus. In fact, it seems that this inoculation technique is not suitable for this type of investigation. On the one hand, the inoculation pressure is too strong. On the other hand, as the virus is in direct contact with the vessels, the technique does not allow to identify resistances that might occur in the first stage of a natural infection.
description: VITIS - Journal of Grapevine Research, Vol. 35 No. 1 (1996): Vitis
Pathogenesis-related proteins in grapevines induced by salicylic acid and Botrytis cinerea
Creators: name: Renault, Anne-Sophie
name: Deloire, A.
name: Bierne, J.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: The grapevine pathogenesis-related proteins (PRs) were studied in order to determine the spatial localization and the temporal production of these inducible proteins. We used leaves of plants obtained from woody cuttings grown in greenhouse. Elicitations were done either with salicylic acid or with strains of Botrytis cinerea. Several PRs extractable at pH 2.8 were found to accumulate in grapevine leaves after salicylic acid treatment or Botrytis infection (SDS-PAGE, coomassie blue). Elicitation with salicylic acid has induced one new protein at about 32 kDa. Botrytis infection has resulted in the accumulation of four major acid-soluble proteins with apparent molecular weights of 27, 32, 34 and 38 kDa. Immunodetections using antisera raised against the tobacco PR-2 family have shown several bands, particularly two bands at 34 and 36 kDa revealed by the anti-2a and present both with salicylic acid and Botrytis.
description: VITIS - Journal of Grapevine Research, Vol. 35 No. 1 (1996): Vitis
Composition of fatty acids, triacylglycerols and polar compounds of different walnut varieties (Juglans regia L.) from Tunisia
Creators: name: I. Bouabdallah
name: I. Bouali
name: E. Martinez-Force
name: A. Albouchi
name: M.C. Perez Camino
name: S. Boukhchina
Publisher: name: Taylor & Francis
Year: 2014
Subjects: subject: Inorganic Chemistry
subject: FOS: Chemical sciences
subject: Biological Sciences
subject: Ecology
subject: FOS: Biological sciences
subject: Chemistry
subject: Earth and Environmental Sciences
subject: Evolutionary Biology
subject: Biotechnology
subject: Microbiology
subject: Biochemistry

description: The chemical composition (total oil content, fatty acids, triacylglycerols (TAGs) and polar compounds) of six walnuts (Juglans regia L.) cultivars (Lauzeronne, Franquette, Hartley, Local pt, Local gd and Parisienne) collected from Mateur (north of Tunisia) was evaluated. The major fatty acids found in the walnut oils are linoleic acid (60.42–65.77%), oleic acid (13.21–19.94%) and linolenic acid (7.61–13%). The TAG species were mainly composed of trilinolein (LLL), dilinoleoyl-linolenoyl-glycerol, dilinoleoyl-oleoyl-glycerol and palmitoyl-dilinoleoyl-glycerol classes. The results revealed that Local pt variety has the highest level of oil (62.56%), linoleic acid (65.77%) and LLL (33.48%). Significant differences among oil samples were observed, therefore showing a great variability in the oil composition among cultivars.
Studies on ''corky rugose wood'' of grapevine and on the diagnosis of grapevine virus B
Creators: name: Bonavia, M.
name: Digiaro, M.
name: Boscia, D.
name: Boari, A.
name: Bottalico, G.
name: Savino, V.
name: Martelli, G. P.
Publisher: name: VITIS - Journal of Grapevine Research
Year: 2015

description: Vines affected by corky rugose wood (CRW), a field syndrome characterized by pronounced cork production by the scion of several grapevine varieties just above the graft union, contain a number of filamentous and isometric phloem-limited viruses, such as grapevine leafroll-associated virus 1, 2, and 3 (GLRaV-1, GLRaV-2, GLRaV-3), grapevine virus A and B (GVA and GVB), and grapevine fleck virus (GFkV). However, the same viruses, with the exception of GVB, are widely represented also in vines with rugose wood without excessive corkyness. Although GVB was found in all vines indexing positive in LN 33 for corky bark disease, iis occurrence in CRW-affected vines was not consistent enough to suggest that it may have a determining role in the induction of this syndrome. Monoclonal antibodies to GVB raised previously were characterized and their possible use for reliable detection of GVB in field-grown vines investigated in detail. A triple antibody sandwich ELISA protocol that under our experimental conditions afforded consistent and repeatable results, was based on the use of crude cortical scraping extracts from mature canes collected in autumn, antibodies from a polyclonal antiserum for plate coating (trapping) and a monoclonal antibody for antigen detection.
description: VITIS - Journal of Grapevine Research, Vol. 35 No. 1 (1996): Vitis