Trypetesa habei Utinomi 1962
Creators: name: Chan, Benny K. K.
name: Cheang, Chi Chiu
name: Chen, I-Han
name: Kolbasov, Gregory A.
Publisher: name: Zenodo
Year: 2013
Subjects: Biodiversity
Taxonomy
Animalia
Arthropoda
Maxillopoda
Apygophora
Trypetesidae
Trypetesa
Trypetesa habei

description: Trypetesa habei Utinomi, 1962
Figure 6 B
Trypetesa habei Utinomi, 1962: 399. — Tomlinson 1969: 129, fig. 35. — Kolbasov 2009: 334, fig. 116.
Material examined. CEL-Acro-sp-261, 1 specimen, in Drupa ricinus occupied by a hermit crab, He-Ping-Dao, Keelung, Taiwan, 9 July 2010.
Diagnosis. (Female) Burrow opening oval, extending as fine narrow slit (Fig. 6 B); opercular bars smooth, without teeth or conspicuous denticles (Fig. 6 B); 3 pairs of terminal cirri, uniramus, 4 joined; caudal appendages absent, mantle sac with orifical knob and 2 palps (Fig. 6 B).
Geographical distribution. Japan, Taiwan.
Remarks. This represents a new record for Taiwan.
Molecular analysis
A total of 25 and 28 partial sequences were obtained for COI (454–469 bp) and 16S (508–16 bp), respectively, from 28 specimens of the six species (Tables 1, 2). COI (h: 0.99 ± 0.01; π: 0.1881 ± 0.0100) exhibits 196 polymorphic sites (179 parsimony informative sites, PIS), while 16S (h: 0.94 ± 0.03; π: 0.17 ± 0.01) demonstrates 183 PIS out of 193 polymorphic sites. Excluding T. habei (due to a single sample), although all five species show high haplotype diversities in the COI dataset, A. bicornis shows the highest nucleotide diversity (Table 2). Additionally, A. bicornis demonstrates the highest haplotype and nucleotide diversities in 16S (Table 2) and intraspecific K2P genetic distances for both genes (Table 3) among all the acrothoracican species. The pairwise K2P distances among species, ranging from 0.132 to 0.288, are relatively uniform compared to the intra-specific distances. From the phylogenetic trees based on the both 16S (Fig. 7 A) and COI (Fig. 7 B) genes, although the statistical supports of the inter-specific relationship are relatively low, each species by their own form a highly significant clade (Fig. 7).
N, total number of specimens examined; n, number of sequences obtained; length, the aligned length of the markers; n h, number of haplotypes; H, haplotype diversity; π, nucleotide diversity; and N.A., not applicable. Collection site of acrothoracican species. Refer to text. Kochlorine hamata was collected in Phuket, Thailand for comparative studies in the present study.
Specimens of B. taiwanus from different substrata, including barnacle and gastropod shells and dead coral skeletons, clustered in the same clade and the differences in K2P distance between specimens from different substrata were <1%.
In Auritoglyptes, specimens from He-Ping-Dao, Keelung, Taiwan formed three distinct clades in the 16S and COI tree (Fig 7). K2P differences among the three clades in the 16S region reached>7%. This differentiation is concordant with the high genetic diversity and the high intra-specific genetic distances observed in this species.
Bt, Balanodytes taiwanus; Au, Auritoglyptes bicornis; Be, Berndtia purpurea; Ko, Kochlorine hamata; Li, Lithoglyptes sp.; Tr, Trypetesa habei; and N.A, not applicable.
description: Published as part of Chan, Benny K. K., Cheang, Chi Chiu, Chen, I-Han & Kolbasov, Gregory A., 2013, Acrothoracican barnacles (Lithoglyptida) in Taiwan, including the taxonomic status of Balanodytes taiwanus Utinomi, 1950 and cryptic diversity of Auritoglyptes bicornis (Aurivillius, 1892), pp. 221-239 in Zootaxa 3694 (3) on pages 233-236, DOI: 10.11646/zootaxa.3694.3.3, http://zenodo.org/record/221152