Pseudamnicola bodoni Delicado, Boulaassafer & Hauffe 2025, sp. nov.
Creators: name: Delicado, Diana
name: Boulaassafer, Khadija
name: Khalloufi, Noureddine
name: Hauffe, Torsten
Publisher: name: Zenodo
Year: 2024
Subjects: Biodiversity
Taxonomy
Animalia
Mollusca
Gastropoda
Littorinimorpha
Hydrobiidae
Pseudamnicola
Pseudamnicola bodoni

description: Pseudamnicola bodoni Delicado, Boulaassafer & Hauffe sp. nov.
(Figs 9, 10)
Pseudamnicola sp. 5 — Delicado et al. 2015
Pseudamnicola sp. 3 — Delicado et al. 2018
Pseudamnicola sp. 3 — Boulaassafer et al. 2020
ZooBank registration: urn:lsid:zoobank.org:act: 536D395B-2699-420F-B2A6-8020BC0BC931
Etymology
Named after Marco Bodon, collector of the species, in recognition of his valuable contribution to the knowledge of the molluscan fauna of Italy.
Type material
Holotype (MNCN 15.05 /200502H) in the MNCN collection and five paratypes in the UGSB collection (UGSB 988). Type locality: a spring on the left bank of the Biedano Stream, Blera, Latium, Italy.
Material studied
A spring on the left bank of the Biedano Stream, Blera, Latium, Italy, 42.2680°N, 12.0256°E, leg. M.B., November 2007, MNCN 15.05 /200502H and UGSB 988 (80% ethanol). A spring in Via Marrucella, Capri Island, Italy, 40.5492°N, 14.2323°E, leg. M.B., March 2004, UGSB 5967 (80% ethanol). A stream near Sperlonga, Latium, Italy, 41.2579°N, 13.4348°E, leg. M.B. and S.C., January 2014, UGSB 16628 (80% ethanol).
Diagnosis
Shell small, ovate-conic, with a short spire; protoconch microsculpture pitted; central radular tooth formula 4-C-4/1-1; bursa copulatrix small, pyriform, with a duct slightly longer than bursal length; SR1 elongate, with a short duct; penis gradually tapering, unpigmented, with many folds over the entire surface and a wide base; nervous system darkly pigmented, elongate (mean RPG ratio =.50).
Description
Shell ovate-conic, whorls 3–3.5, height 2–2.5 mm (Fig. 9A–C; Supporting Information, Table S3). Periostracum yellowish. Protoconch ~400 µm wide, whorls 1.5; nucleus ~125 µm wide; protoconch microsculpture pitted (Fig. 9G). Teleoconch whorls moderately convex, with deep sutures; body whorl large, occupying about three-quarters of total shell length. Aperture longer than wide, slightly oval; inner lip thicker than outer lip; peristome margin simple, straight (Fig. 9D). Umbilicus narrow, not covered by the inner lip. Holotype: SL = 2.21 mm, SW = 1.81 mm, AL = 1.26 mm, and AW = 0.86 mm.
Operculum oval, brownish, about two and a half whorls; muscle attachment area oval and located near the nucleus (Fig. 9E, F).
Radular length intermediate, ~600 µm (~25% of total shell length), with ~60 rows of teeth (Fig. 9H). Central tooth formula 4-C-4/1-1 (Fig. 9I); basal tongue V-shaped, length about equal to lateral margin. Lateral tooth formula 3-C-3. Inner marginal teeth having ~25 tapered cusps, shortening towards the base. Outer marginal teeth with ~20 sharp cusps (Fig. 9J).
Animal darkly pigmented except for neck and tentacles (Fig. 10F). Snout as long as wide, with medium distal lobation; foot size intermediate, with dorsal pigmentation. Ctenidium with 17–19 well-developed gill filaments, occupying ~50% of pallial cavity length and posteriorly positioned. Osphradium of intermediate width and opposite middle of ctenidium (Fig. 10A). Stomach as long as wide, with two chambers almost equal in size and a medium-sized posterior caecum; style sac longer than wide, surrounded by an unpigmented intestine (Fig. 10B; Supporting Information, Table S4). Nervous system elongate (mean RPG ratio =.50), darkly pigmented, darker on ganglia than on connectives and commissures; cerebral ganglia approximately equal in size (Fig. 10C).
Female glandular oviduct approximately four times as long as wide. Albumen gland shorter than capsule gland. Bursa copulatrix small, pyriform, longer than wide. Bursal duct longer than bursal length. Renal oviduct black-pigmented until the area above the insertion of seminal receptacle, making one or two loops. Seminal receptacle elongate, with a short duct, joining renal oviduct at the insertion point with bursal duct (Fig. 10D, E; Supporting Information, Table S5).
Male genitalia with a prostate gland approximately three times as long as wide, bean-shaped; seminal duct entering the middle-posterior region; pallial vas deferens emerging close to its anterior edge (Fig. 10H). Penis shorter than head, gradually tapering, unpigmented, with many folds over the entire surface (Fig. 10F, G; Supporting Information, Table S6); base wide; attached well behind the right eye; penial duct narrow, curved, coursing close to outer edge.
Habitat
The species lives in coastal running waters, mainly in small springs.
Remarks
The few individuals available for the study showed little variation in shell features (Fig. 9A–C). The species was first discovered by DNA-based phylogenetic analyses (Delicado et al. 2015), but the comprising populations could not be assigned to any of the nominal species owing to a lack of morphological information. In our study, all DNA-based species delimitation methods (except the ABGD for 16S) recovered this species as distinct from P. lucensis, P. orsinii, and P. argentariensis, which also occur on the Apennine Peninsula and adjacent islands (Issel 1866, Giusti 1976, Falkner and Boeters 2003). Pseudamnicola bodoni differs from these geographically proximate species in the combination of a short spire, short seminal receptacle, short penis, and average sequence divergence of 4.8%–7.8% for COI. It differs from the Sicilian populations of P. moussonii and P. reticulatus in having a more globose shell, a larger number of cusps on the marginal radular teeth, a shorter penis, and an average sequence divergence of 6.1%–7.6% for COI.
description: Published as part of Delicado, Diana, Boulaassafer, Khadija, Khalloufi, Noureddine & Hauffe, Torsten, 2025, A holistic perspective on species delimitation outperforms all methods based on single data types in freshwater gastropods (Caenogastropoda: Hydrobiidae: Pseudamnicola), pp. 1-31 in Zoological Journal of the Linnean Society 203 (1) on pages 18-21, DOI: 10.1093/zoolinnean/zlae010, http://zenodo.org/record/14774861