title: ÐÐ»Ð¸ÑÐ½Ð¸Ðµ Ð²ÑÑÐ¾ÐºÐ¾ÑÑÑÐºÑÐ¾Ð·Ð½Ð¾Ð³Ð¾ ÑÐ°ÑÐ¸Ð¾Ð½Ð° Ð½Ð° Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸ÑÐµÑÐºÐ¸Ðµ Ð¸ Ð¿Ð¾Ð²ÐµÐ´ÐµÐ½ÑÐµÑÐºÐ¸Ðµ Ð¿Ð¾ÐºÐ°Ð·Ð°ÑÐµÐ»Ð¸ ÐºÑÑÑ Ð½Ð¾ÐºÐ°ÑÑÐ½Ð¾Ð¹ Ð»Ð¸Ð½Ð¸Ð¸ DAT-KO
title: Ð²ÑÐ¿ÑÑÐºÐ½Ð°Ñ ÐºÐ²Ð°Ð»Ð¸ÑÐ¸ÐºÐ°ÑÐ¸Ð¾Ð½Ð½Ð°Ñ ÑÐ°Ð±Ð¾ÑÐ° Ð±Ð°ÐºÐ°Ð»Ð°Ð²ÑÐ°
titleType: Subtitle
title: The effect of a high-fructose diet on the metabolic and behavioral indicators of DAT-KO knockout rats
Creators: name: ÐÐ¾Ð»ÑÑÐ°ÐºÐ¾Ð²Ð°, ÐÐ°ÑÐ¸Ñ
Publisher: name: Ð¡Ð°Ð½ÐºÑ-ÐÐµÑÐµÑÐ±ÑÑÐ³ÑÐºÐ¸Ð¹ Ð¿Ð¾Ð»Ð¸ÑÐµÑÐ½Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ½Ð¸Ð²ÐµÑÑÐ¸ÑÐµÑ ÐÐµÑÑÐ° ÐÐµÐ»Ð¸ÐºÐ¾Ð³Ð¾
Year: 2020
Subjects: Ð¾Ð¶Ð¸ÑÐµÐ½Ð¸Ðµ
Ð´Ð¾ÑÐ°Ð¼Ð¸Ð½
ÐÐÐ¢
Ð³Ð¸Ð¿ÐµÑÐ°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ
Ð½Ð¾ÐºÐ°ÑÑÐ½Ð°Ñ Ð»Ð¸Ð½Ð¸Ñ
ÑÑÑÐºÑÐ¾Ð·Ð°
Ð²ÑÑÐ¾ÐºÐ¾ÑÑÑÐºÑÐ¾Ð·Ð½ÑÐ¹ ÑÐ°ÑÐ¸Ð¾Ð½
obesity
dopamine
DAT
hyperactivity
knockout line
fructose
high fructose diet

description: ÐÐ¶Ð¸ÑÐµÐ½Ð¸Ðµ, Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸ÑÐµÑÐºÐ¸Ð¹ ÑÐ¸Ð½Ð´ÑÐ¾Ð¼, Ð°ÑÐµÑÐ¾ÑÐºÐ»ÐµÑÐ¾Ð·, Ð³Ð¸Ð¿ÐµÑÐ»Ð¸Ð¿Ð¸Ð´ÐµÐ¼Ð¸Ñ Ð¸ Ð´ÑÑÐ³Ð¸Ðµ Ð°Ð»Ð¸Ð¼ÐµÐ½ÑÐ°ÑÐ½Ð¾-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼ÑÐµ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ñ ÑÐ²Ð»ÑÑÑÑÑ Ð½Ð° ÑÐµÐ³Ð¾Ð´Ð½ÑÑÐ½Ð¸Ð¹ Ð´ÐµÐ½Ñ Ð½Ð°Ð¸Ð±Ð¾Ð»ÐµÐµ ÑÐ°ÑÐ¿ÑÐ¾ÑÑÑÐ°Ð½ÐµÐ½Ð½ÑÐ¼Ð¸ Ð² Ð¼Ð¸ÑÐµ Ð°Ð»Ð¸Ð¼ÐµÐ½ÑÐ°ÑÐ½Ð¾-Ð·Ð°Ð²Ð¸ÑÐ¸Ð¼ÑÐ¼Ð¸ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸ÑÐ¼Ð¸. ÐÐ´Ð½Ð¸Ð¼ Ð¸Ð· ÐÑÐ½Ð¾Ð²Ð½ÑÑ ÑÐ°ÐºÑÐ¾ÑÐ¾Ð¼ ÑÐ¸ÑÐºÐ° ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ñ ÑÑÐ¸Ñ Ð±Ð¾Ð»ÐµÐ·Ð½ÐµÐ¹ ÑÐ²Ð»ÑÐµÑÑÑ Ð½ÐµÐ¿ÑÐ°Ð²Ð¸Ð»ÑÐ½Ð¾Ðµ Ð¿Ð¸ÑÐ°Ð½Ð¸Ðµ. ÐÐ½Ð¾Ð³Ð¸Ðµ Ð¼ÐµÑÐ¾Ð´Ñ Ð»ÐµÑÐµÐ½Ð¸Ñ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸Ð¹ Ð¾Ð±Ð¼ÐµÐ½Ð° Ð²ÐµÑÐµÑÑÐ² Ð·Ð°ÑÐ°ÑÑÑÑ Ð¾ÐºÐ°Ð·ÑÐ²Ð°ÑÑÑÑ Ð¼Ð°Ð»Ð¾ÑÑÑÐµÐºÑÐ¸Ð²Ð½Ñ. Ð ÑÐ²ÑÐ·Ð¸ Ñ ÑÑÐ¸Ð¼ ÐµÑÑÑ Ð½ÐµÐ¾Ð±ÑÐ¾Ð´Ð¸Ð¼Ð¾ÑÑÑ Ð¿Ð¾Ð¸ÑÐºÐ° Ð½Ð¾Ð²ÑÑ ÑÑÑÐ°ÑÐµÐ³Ð¸Ð¹, ÑÐ°Ðº ÐºÐ°Ðº Ð¸Ð·Ð±ÑÑÐ¾ÑÐ½ÑÐ¹ Ð²ÐµÑ Ð¸ Ð´ÑÑÐ³Ð¸Ðµ Ð¿Ð¾ÑÐµÐ½ÑÐ¸Ð°Ð»ÑÐ½Ð¾ Ð½ÐµÐ¶ÐµÐ»Ð°ÑÐµÐ»ÑÐ½ÑÐµ Ð½Ð°ÑÑÑÐµÐ½Ð¸Ñ Ð¼Ð¾Ð³ÑÑ Ð¿ÑÐ¸Ð²Ð¾Ð´Ð¸ÑÑ Ðº ÑÐµÑÑÑÐ·Ð½ÑÐ¼ Ð¿Ð¾ÑÐ»ÐµÐ´ÑÑÐ²Ð¸ÑÐ¼ Ð´Ð»Ñ Ð·Ð´Ð¾ÑÐ¾Ð²ÑÑ. ÐÑÑÐ³Ð¸Ð¼ Ð²Ð°Ð¶Ð½ÑÐ¼ ÑÐ°ÐºÑÐ¾ÑÐ°Ð¼Ð¸ ÑÐ¸ÑÐºÐ° ÑÐ°Ð·Ð²Ð¸ÑÐ¸Ñ Ð°Ð»Ð¸Ð¼ÐµÐ½ÑÐ°ÑÐ½ÑÑ Ð¸ Ð½ÐµÐ¹ÑÐ¾Ð´ÐµÐ³ÐµÐ½ÐµÑÐ°ÑÐ¸Ð²Ð½ÑÑ Ð¿Ð°ÑÐ¾Ð»Ð¾Ð³Ð¸ÑÐµÑÐºÐ¸Ñ ÑÐ¾ÑÑÐ¾ÑÐ½Ð¸Ð¹ ÑÐ²Ð»ÑÑÑÑÑ ÑÐ±Ð¾Ð¸ Ð² ÑÐ°Ð±Ð¾ÑÐµ Ð´Ð¾ÑÐ°Ð¼Ð¸Ð½Ð¾Ð²ÑÑ ÑÐ¸ÑÑÐµÐ¼ Ð¼Ð¾Ð³ÑÑ Ð¿ÑÐ¸Ð²Ð¾Ð´Ð¸ÑÑ Ðº ÑÐ°ÐºÐ¸Ð¼ Ð·Ð°Ð±Ð¾Ð»ÐµÐ²Ð°Ð½Ð¸ÑÐ¼, ÑÐ°ÐºÐ¸Ð¼ ÐºÐ°Ðº Ð±Ð¾Ð»ÐµÐ·Ð½Ñ ÐÐ°ÑÐºÐ¸Ð½ÑÐ¾Ð½Ð°, ÑÐ¸Ð·Ð¾ÑÑÐµÐ½Ð¸Ñ, Ð°ÑÑÐ¸Ð·Ð¼, Ð¡ÐÐÐ. 
Ð¦ÐµÐ»ÑÑ Ð´Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð¸ÑÑÐ»ÐµÐ´Ð¾Ð²Ð°Ð½Ð¸Ñ Ð±ÑÐ»Ð¾ Ð¸Ð·ÑÑÐµÐ½Ð¸Ðµ Ð²Ð»Ð¸ÑÐ½Ð¸Ñ Ð²ÑÑÐ¾ÐºÐ¾ÑÑÑÐºÑÐ¾Ð·Ð½Ð¾Ð³Ð¾ ÑÐ°ÑÐ¸Ð¾Ð½Ð° Ð½Ð° Ð¸Ð½ÑÐµÐ³ÑÐ°Ð»ÑÐ½ÑÐµ, Ð±Ð¸Ð¾ÑÐ¸Ð¼Ð¸ÑÐµÑÐºÐ¸Ðµ Ð¸ Ð¿Ð¾Ð²ÐµÐ´ÐµÐ½ÑÐµÑÐºÐ¸Ðµ Ð¿Ð¾ÐºÐ°Ð·Ð°ÑÐµÐ»Ð¸ ÐºÑÑÑ, Ð¾Ð¿Ð¾ÑÑÐµÐ´Ð¾Ð²Ð°Ð½Ð½ÑÐµ Ð³ÐµÐ½ÐµÑÐ¸ÑÐµÑÐºÐ¸ Ð¿Ð¾Ð²ÑÑÐµÐ½Ð½ÑÐ¼ ÑÑÐ¾Ð²Ð½ÐµÐ¼ Ð´Ð¾ÑÐ°Ð¼Ð¸Ð½Ð° Ð² Ð¼Ð¾Ð·Ð³Ðµ, Ñ Ð¸ÑÐ¿Ð¾Ð»ÑÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ in vivo Ð¼Ð¾Ð´ÐµÐ»ÐµÐ¹ Ð´Ð¸ÐµÑ-Ð¸Ð½Ð´ÑÑÐ¸ÑÐ¾Ð²Ð°Ð½Ð½Ð¾Ð³Ð¾ Ð¾Ð¶Ð¸ÑÐµÐ½Ð¸Ñ Ð¸ Ð½Ð¾ÐºÐ°ÑÑÐ½Ð¾Ð¹ Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð³Ð¸Ð¿ÐµÑÐ°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸.
ÐÑÐ½Ð¾Ð²Ð½ÑÐµ Ð¿Ð¾ÑÑÐ°Ð²Ð»ÐµÐ½Ð½ÑÐµ Ð¸ ÑÐµÑÐµÐ½Ð½ÑÐµ Ð² ÑÐ¾Ð´Ðµ Ð´Ð°Ð½Ð½Ð¾Ð¹ ÑÐ°Ð±Ð¾ÑÑ Ð·Ð°Ð´Ð°ÑÐ¸ Ð²ÐºÐ»ÑÑÐ°Ð»Ð¸ Ð² ÑÐµÐ±Ñ:
1. ÐÐ¾ÑÐ¿ÑÐ¾Ð¸Ð·Ð²ÐµÑÑÐ¸ in vivo Ð¼Ð¾Ð´ÐµÐ»Ð¸ Ð¼ÐµÑÐ°Ð±Ð¾Ð»Ð¸ÑÐµÑÐºÐ¸Ñ Ð½Ð°ÑÑÑÐµÐ½Ð¸Ð¹ (Ð¾Ð¶Ð¸ÑÐµÐ½Ð¸Ñ) Ð½Ð° ÐºÑÑÑÐ°Ñ Ð°ÑÑÐ±ÑÐµÐ´Ð½Ð¾Ð¹ Ð»Ð¸Ð½Ð¸Ð¸ Wistar.
2. ÐÑÑÐ»ÐµÐ´Ð¾Ð²Ð°ÑÑ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ Ð¸Ð·Ð±ÑÑÐºÐ° ÑÐµÐ½Ð¸Ð»Ð°Ð»Ð°Ð½Ð¸Ð½Ð° Ð¸ ÑÐ¸ÑÐ¾Ð·Ð¸Ð½Ð° (Ð¿ÑÐµÐ´ÑÐµÑÑÐ²ÐµÐ½Ð½Ð¸ÐºÐ¾Ð² Ð´Ð¾ÑÐ°Ð¼Ð¸Ð½Ð°) Ð² ÑÐ°ÑÐ¸Ð¾Ð½Ðµ Ð½Ð° Ð»Ð¾ÐºÐ¾Ð¼Ð¾ÑÐ¾ÑÐ½ÑÑ Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ ÐºÑÑÑ Ð°ÑÑÐ±ÑÐµÐ´Ð½Ð¾Ð¹ Ð»Ð¸Ð½Ð¸Ð¸ Wistar.
3. ÐÐ·ÑÑÐ¸ÑÑ Ð²Ð»Ð¸ÑÐ½Ð¸Ðµ 20%-Ð³Ð¾ ÑÐ°ÑÑÐ²Ð¾ÑÐ° ÑÑÑÐºÑÐ¾Ð·Ñ Ð² ÑÐ°ÑÐ¸Ð¾Ð½Ðµ Ð½Ð° Ð¸Ð½ÑÐµÐ³ÑÐ°Ð»ÑÐ½ÑÐµ (Ð¼Ð°ÑÑÐ° ÑÐµÐ»Ð°, Ð¾ÑÐ½Ð¾ÑÐ¸ÑÐµÐ»ÑÐ½Ð°Ñ Ð¼Ð°ÑÑÐ° Ð¾ÑÐ³Ð°Ð½Ð¾Ð² Ð¸ ÑÐºÐ°Ð½ÐµÐ¹, ÐÐÐ¢ Ð¸ Ð´Ñ.), Ð±Ð¸Ð¾ÑÐ¸Ð¼Ð¸ÑÐµÑÐºÐ¸Ðµ (Ð°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÑ Ð³Ð»ÑÑÐ°ÑÐ¸Ð¾Ð½-S-ÑÑÐ°Ð½ÑÑÐµÑÐ°Ð·Ñ, Ð³Ð»ÑÑÐ°ÑÐ¸Ð¾Ð½ÑÐµÐ´ÑÐºÑÐ°Ð·Ñ Ð² ÑÑÐ¸ÑÑÐ¾ÑÐ¸ÑÐ°Ñ Ð¸ ÐºÐ°ÑÐ°Ð»Ð°Ð·Ñ Ð² ÑÑÐ²Ð¾ÑÐ¾ÑÐºÐµ ÐºÑÐ¾Ð²Ð¸) Ð¸ Ð¿Ð¾Ð²ÐµÐ´ÐµÐ½ÑÐµÑÐºÐ¸Ðµ (ÑÐµÑÑÑ Â«ÐÑÐºÑÑÑÐ¾Ðµ Ð¿Ð¾Ð»ÐµÂ», Â«ÐÑÐ¸Ð¿Ð¾Ð´Ð½ÑÑÑÐ¹ ÐºÑÐµÑÑÐ¾Ð¾Ð±ÑÐ°Ð·Ð½ÑÐ¹ Ð»Ð°Ð±Ð¸ÑÐ¸Ð½ÑÂ» Ð¸ Â«Ð£ÑÐ»Ð¾Ð²Ð½ÑÐ¹ ÑÐµÑÐ»ÐµÐºÑ Ð°ÐºÑÐ¸Ð²Ð½Ð¾Ð³Ð¾ Ð¸Ð·Ð±ÐµÐ³Ð°Ð½Ð¸ÑÂ») Ð¿Ð¾ÐºÐ°Ð·Ð°ÑÐµÐ»Ð¸ ÐºÑÑÑ Ð°ÑÑÐ±ÑÐµÐ´Ð½Ð¾Ð¹ Ð»Ð¸Ð½Ð¸Ð¸ Wistar (in vivo Ð¼Ð¾Ð´ÐµÐ»Ñ Ð¾Ð¶Ð¸ÑÐµÐ½Ð¸Ñ) Ð¸ ÐºÑÑÑ Ð½Ð¾ÐºÐ°ÑÑÐ½Ð¾Ð¹ Ð»Ð¸Ð½Ð¸Ð¸ DAT-KO (in vivo Ð¼Ð¾Ð´ÐµÐ»Ñ Ð³Ð¸Ð¿ÐµÑÐ°ÐºÑÐ¸Ð²Ð½Ð¾ÑÑÐ¸).
description: Obesity, metabolic syndrome, atherosclerosis, hyperlipidemia and other alimentary-dependent diseases are by far the most common alimentary-dependent diseases in the world. One of the main risk factors for the development of these diseases is malnutrition. Many methods of treating metabolic diseases are often ineffective. In this regard, there is a need for new strategies searching, as overweight and other potentially unwanted disorders can lead to serious health consequences. Other important risk factors for the development of nutritional and neurodegenerative pathological conditions development are malfunctions of dopamine systems that can lead to diseases such as Parkinson's disease, schizophrenia, autism, ADHD.
The aim of this research was studying the effect of a high-fructose diet on the integral, biochemical, and behavioral parameters of rats mediated by a genetically elevated level of dopamine in the brain using in vivo models of diet-induced obesity and a knock-out hyperactivity model.
The main tasks that were set and solved during this work included:
1. To reproduce in vivo models of metabolic disorders (obesity) in rats of the outbred Wistar strain.
2. To study the effects of excess phenylalanine and tyrosine (dopamine precursors) in the diet on the locomotor activity of Wistar outbred rats.
3. To study the effects of a 20% solution of fructose in the diet on integral (body weight, relative mass of organs and tissues, BMI, etc.), biochemical (activity of glutathione-S-transferase, glutathione reductase in red blood cells and catalase in blood serum) and behavioral (tests âOpen fieldâ, âElevated cruciform labyrinthâ and âConditional reflex of active avoidanceâ) indicators of Wistar outbred rats (in vivo obesity model) and DAT-KO knockout rats (in vivo hyperactivity model).