Sex: M
Vaccine: COVID19
Symptoms: SARS-CoV-2 antibody test, Serology test, Vaccination failure, Vital signs measurement
Clinical narrative: COVID-19 vaccine failure; Covid-19; This is a literature report.  The authors report another case of vaccine failure, this time in a patient on rituximab, an anti-CD20 mAb. A 73-year-old male presented with nasal congestion, productive cough, myalgias and malaise of 4 days duration. Five weeks before, he had completed a two-dose series of the Pfizer-BioNTech COVID-19 vaccine given 21 days apart. He had a history of cryptogenic organizing pneumonia, intermittent asthma, type II diabetes mellitus, hyperlipidaemia and coronary artery disease. He was diagnosed with cryptogenic organizing pneumonia 2 years earlier, which was initially treated with prednisone and switched to rituximab owing to an inadequate treatment response. He was scheduled for i.v. rituximab infusion therapy at a dose of 375mg/m2 every 6months, and his last dose was administered 10 days after he had received the second dose of the vaccine. His medications included metoprolol succinate, atorvastatin, ezetimibe, metformin, sitagliptin, and a budesonide and formoterol inhaler. There were no known allergies. The patient is retired and lives in City. Since the start of the COVID-19 pandemic, he had restricted his participation in social activities and wore a face mask in public. He denied any history of alcohol, tobacco or illicit drug use. On examination, the patient appeared comfortable. Vital signs were within normal limits. His respiratory effort and breath sounds were normal, as was the remainder of the physical examination. He tested positive for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) via an RT-PCR nasopharyngeal swab. Given the high risk of progression to severe COVID-19 disease owing to his age and comorbidities, bamlanivimab and etesivimab, an mAb cocktail against SARS-CoV-2 was administered uneventfully. Serological testing was performed before the mAb cocktail infusion with two separate assays, assessing the immunological response to the spike and nucleocapsid protein of SARS-CoV-2, respectively. He tested negative for antibodies to both the spike (S) and nucleocapsid (N) antigens. He reported resolution of his symptoms 3 days after the infusion. Rituximab is an anti-CD20 mAb that binds to the CD20 antigen on the B cell surface, activating complement-dependent B cell cytotoxicity. B cells are believed to play a role in the development and progression of some autoimmune diseases. Signs and symptoms of those diseases are reduced by targeting B cells, and the progression of structural damage is delayed. Rituximab is widely used in patients with autoimmune disorders, haematological malignancies and vasculitides. The B-cell-depleting nature of rituximab therapy can lead to an impaired humoral immune response to vaccination. Studies have shown a blunted influenza vaccine response in patients on rituximab. The effect was most pronounced if vaccinated <2months, with an improved response if vaccinated >6months after rituximab therapy. In the vaccine clinical guidance by the ACR, patients with a low or mitigatable risk of COVID-19 should have their vaccination scheduled ~4weeks before their next scheduled rituximab cycle; and after the completion of vaccination, should delay rituximab for 2-4weeks after the second vaccine dose, if disease activity allows. A recent review article in Rheumatology recommended avoiding vaccination after rituximab, ideally for 6months; and if vaccination is imminent, consider delaying rituximab if there are no risks of organ failure or disease flare. In this patient, rituximab was administered 10days after the last dose of the vaccine, and the patient developed clinical disease 5 weeks after completing vaccination. The absence of spike protein antibodies indicates an inadequate humoral response after 5 weeks. Peak antibody response is expected 2weeks after the second dose of the COVID-19 vaccine. The two-dose regimen of the vaccine confers a 95% protection against infection, and this could explain vaccine failure rather than use of rituximab. The half-life and prolonged B-cell-depleting effects of rituximab make it challenging to obtain an optimal window for vaccination. T-cell-mediated immunity after vaccination could have played a role in the mild nature of his illness, which was not accounted for by measuring only the humoral antibody response. This case highlights the challenges in vaccinating patients on immunosuppressive drugs. Finding an optimal window and obtaining post-vaccination antibody titres might be beneficial. Patients should continue to maintain a high adherence to preventive measures against COVID-19. Further studies are needed to assess the efficacy of COVID-19 vaccine in patients on immunosuppressive therapies.; Sender's Comments: As there is limited information in the case provided, the causal association between the reported events Vaccination failure and Covid-19 and the suspect drug cannot be excluded. The case will be reassessed once new information is available.