Title: The molecular biology of insect disease vectors : a methods manual /
Authors: Crampton, Julian M.; Beard, C. B. 1957-; Louis, C.; Crampton, Julian M.; Beard, C. B. 1957-; Louis, C.
Publisher: Chapman and Hall,
Year: 1997
Subjects: Insecta; Insect Vectors genetics; Genetic Techniques veterinary; Culture Techniques veterinary; Animals, Laboratory; Insect Vectors parasitology; Insects Molecular aspects; Vector control; Insects as carriers of disease
Abstract: The overall aim of this text is to provide a source book of detailed protocols covering the important techniques used in molecular entomology for those wishing to enter this field. The text is made up of 46 chapters, each covering a specific methodology and authored by an invited expert. The chapters are written such that a competent scientist who is unfamiliar with the method can carry out the technique successfully, either at the first attempt, or with a little practice, by simply following the detailed procedures. Each chapter includes: an introductory narrative; a detailed list of materials and equipment required; a detailed, step-by-step protocol; a section of explanatory notes; a bibliography. The chapters are organised under section headings to provide a logical structure to the text, progressing from insect maintenance and infection with parasites, through basic molecular techniques, to genome mapping and transgenics. This methods manual will provide a valuable resource to assist in the development of this rapidly expanding field of research and scholarship.
Contents: Care and maintenance of anopheline mosquito colonies / Mark Q. Benedict -- Care and maintenance of Aedes mosquito colonies / Leonard E. Munsterrnann -- Care and maintenance of phlebotomine sandfly colonies / Govind B. Modi -- Care and maintenance of blackfly colonies / Eddie W. Cupp and Frank B. Ramberg -- Care and maintenance of tsetse colonies / Ronald H. Goading, Udo Feldmann and Alan S. Robinson -- Care and maintenance of triatomine colonies / Patricia de Azambuja and Eloi S. Garcia -- Infection of mosquitoes with rodent malaria / Robert E. Sinden -- Infection of mosquitoes with primate malaria / William E. Collins -- Infection of mosquitoes with filaria / Harold Townson -- Infection of phlebotomine sandflies with Leishmania / Paul A. Bates -- Infection of blackflies with Onchocerca / Albert E. Bianco and Peter J. Ham -- Infection of tsetse with Trypanosoma brucei rhodesiense and T.b. gambiense / Ian Maudlin -- Infection of triatomines with Trypanosoma cruzi / Eloi S. Garcia and Patricia de Azambuja -- Isolation of genomic DNA / Christos Louis -- Isolation and analysis of RNA from insect vectors / Margaret A. Hughes -- Determination of insect genome size and complexity by reassociation kinetics / Melanie J. Palmer and William C. Black IV -- Constructing and screening lambda libraries / Margaret A. Hughes -- Constructing and screening cosmid libraries / Kostas D. Mathiopoulos -- Constructing and screening cDNA libraries / Kostas D. Mathiopoulos -- Southern/northern blotting and hybridization techniques / Inga Siden-Kiamos -- The polymerase chain reaction (PCR) and RT-PCR / Bruno Arca and Charalambos Savakis -- Differential display of mRNA / George Dimopoulos and Christos Louis -- Localization of RNA transcripts by in situ hybridization / Philip W. Ingham and Trevor Jowett -- In situ localization of proteins in whole mounted tissue / Marcos A. Gonzalez-Gaitan and Herbert Jackle -- DNA sequencing and primer extension mapping / Hans-Michael Muller -- RFLP analysis of insect genomes / David W. Severson -- Microsatellite mapping of insect genomes / Liangbiao Zheng -- Polytene chromosome preparation from anopheline mosquitoes / Alessandra della Torre -- In situ hybridization to Anopheles polytene chromosomes / Vasantha Kumar, Anthony J. Cornel and Odette Mukabayire -- Microdissection and microamplification of dipteran polytene chromosomes / Robert D.C. Saunders -- RAPD-PCR and SSCP analysis for insect population genetic studies / William C. Black IV and Nancy M. DuTeau -- PCR amplification of insect ribosomal DNA / Susan M. Paskewitz and Frank H. Collins -- Generation and use of species-specific DNA probes for insect vector identification / Julian M. Crampton and Susannah M. Hill -- Cloning and detection of insecticide resistance genes / Nicole Pasteur, Michel Raymond, Francois Rousset, Jean-Baptiste Berge, Marcel Amichot, David Pauron, Andre Cuany and Didier Fournier -- Microinjection of mosquito embryos / Alison C. Morris -- Transfection of cultured mosquito cells / Ann Marie Fallon -- Viral transducing vectors for mosquitoes / Jon O. Carlson, Boris N. Afanasiev, Stephen Higgs, Tomoyo Matsubara and Jane C. Burns -- Viral expression systems and viral infections in insects / Stephen Higgs, Ken E. Olson, Kurt I. Kamrud, Ann M. Powers and Barry J. Beaty -- Establishing primary cell cultures from disease vectors and maintenance of continuous cell lines / Colin J. Leake -- Culture and analysis of insect salivary glands / Alison C. Morris and Anthony A. James -- Culture and analysis of the insect fat body / Alexander S. Raikhel, Kirk W. Deitsch and Thomas W. Sappington -- Culture and analysis of insect ovaries / Thomas W. Sappington, Mark R. Brown and Alexander S. Raikhel -- Culture and analysis of insect midgut / Hans-Michael Muller and Andrea Crisanti -- Isolation and culture of tsetse secondary endosymbionts / Susan C. Welburn and Colin Dale -- Genetic manipulation of insect symbionts / C. Ben Beard and Serap Aksoy -- PCR-based detection and identification of insect symbionts / Scott L. O'Neill.