Title: Apoptosis methods and protocols /
Authors: Brady, Hugh J. M.
Publisher: Humana Press,
Year: 2004
Subjects: Apoptosis physiology; DNA Damage physiology; Apoptosis Laboratory manuals.; Molecular biology Laboratory manuals; DNA Damage physiology Laboratory Manuals.; Apoptosis physiology Laboratory Manuals.
Abstract: The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research. In this book, expert laboratorians describe in step-by-step detail the techniques they have perfected to investigate the critical steps involved in the apoptotic process.
Contents: Measurement of Apoptosis by DNA Fragmentation / Demetrius Matassov, Terri Kagan, Julie Leblanc, Marianna Sikorska, and Zahra Zakeri -- Measurement of Caspase Activity in Cells Undergoing Apoptosis / Sharad Kumar -- Flow Cytometry-Based Methods for Apoptosis Detection in Lymphoid Cells / Owen Williams -- Methods of Analyzing Chromatin Changes Accompanying Apoptosis of Target Cells in Killer Cell Assays / Richard C. Duke -- Detecting and Quantifying Apoptosis in Tissue Sections / Vicki Save, Peter A. Hall, and Philip J. Coates -- Immunoassay for Single-Stranded DNA in Apoptotic Cells / Oskar S. Frankfurt -- Methods to Measure Membrane Potential and Permeability Transition in Mitochondria During Apoptosis / Naoufal Zamzami and Guido Kroemer -- Measurement of Changes in Intracellular Calcium During Apoptosis / David J. McConkey and Leta Nutt -- Measurement of Changes in Apoptosis and Cell Cycle Regulatory Kinase Cdk2 / Gabriel Gil-Gómez --^