Title: Breast cancer research protocols /
Authors: Brooks, Susan A.; Harris, Adrian, 1950-
Publisher: Humana Press,
Year: 2006
Subjects: Breast Cancer Laboratory manuals; Breast Neoplasms genetics; Breast Neoplasms pathology; Biopsy methods; Clinical Protocols methods.; Genetic Techniques; Clinical Protocols
Abstract: A collection of both well-established and cutting-edge methods for investigating breast cancer biology not only in the laboratory, but also in clinical settings. These readily reproducible techniques solve a variety of problems, ranging from how to collect, store, and prepare human breast tumor samples for analysis, to analyzing cells in vivo and in vitro. Additional chapters address the technology of handling biopsies, new methods for analyzing genes and gene expression, markers of clinical outcome and progress, analysis of tumor-derived proteins and antigens, validating targets, and investigating the biology of newly discovered genes.
Contents: Breast tissue banking: collection, handling, storage, and release of tissue for breast cancer research / Linda Snell and Peter H. Watson -- Frozen biopsy collection and storage / Russell D. Leek -- Serial biopsies/FNAs and their assessment / Irene Boeddinghaus and Stephen R.D. Johnson -- Breast tissue microarrays / Robert J. Springall and Cheryl E. Gillett -- Preparation of tumor homogenates for subsequent preparation of cytosols, membrane fractions, RNA, and DNA / Naomi Robertson and Russell Leek.