Title: The logic of biochemical sequencing /
Authors: Blackman, David S.
Publisher: CRC Press,
Year: 1994
Subjects: Sequence Analysis; Logic; Amino Acid Sequence; Amino acid sequence Philosophy.; Logic; Analysis (Philosophy); Amino Acid Sequence Problems and Exercises.; Logic Problems and Exercises.; Sequence Analysis Problems and Exercises.
Abstract: "The Logic of Biochemical Sequencing examines how to determine the primary structures of proteins and DNA and use them to stimulate the process of logical problem-solving. It concentrates on sequencing work and stresses the thought processes needed to make sense of what might otherwise be indecipherable data. The book also introduces "biocryptography," which serves as a basis for four short stories that use the results of sequence determinations to provide clues to higher order problems." "Problems in the book range from elementary to difficult, and solutions to all problems are provided, with many of them completely worked out. The book will be an excellent supplementary text for students in a full-year biochemistry course, as well as for biochemists and molecular biologists."--Jacket.
Contents: 1. End Group Analysis of Proteins. Reactions of the Amino Group. Reactions of the Carboxyl Group -- 2. Limited Hydrolysis of Proteins. Cyanogen Bromide. Trypsin. Chymotrypsin. Elastase. Pepsin -- 3. Base Sequencing of DNA. The Maxam-Gilbert Method. The Sanger Method -- 4. Analysis of DNA by Restriction Mapping. Single Enzyme Digests. Multiple Enzyme Digests -- 5. Additional Problems -- 6. Adventures in Sequencing. Biocryptography -- A New Science. A Night at the Opera (House). From Baltimore with Love. Summertime, and Is the Living Easy?