Title: Quantitative PCR protocols /
Authors: Kochanowski, Bernd.; Reischl, Udo.
Publisher: Humana Press,
Year: 1999
Subjects: Polymerase chain reaction Laboratory manuals.; Polymerase Chain Reaction methods; DNA analysis; RNA analysis
Contents: TABLE OF CONTENTS -- Quantitative PCR : a survey of the present technology -- General principles of quantitative PCR -- Effects of collection, processing, and storage on RNA detection and quantification -- Quantitative RT-PCR -- Kinetic quantitative PCR vs end-point quantitative PCR with internal standard -- Comparison of competitive PCR and positive control-based PCR -- End-point titration-PCR and positive PCR and positive control-based PCR -- End-point titration-PCR for quantitation of cytomegalovirus DNA -- Analysis of amplified DNA moleculkes by capillary electrophoresis and laser induced fluorescence -- Competitor calibration and analysis of competitive amplified PCR products by high-performance liquid chromatography (HPLC) -- Quantifying amplicons with ELISA.