Title: Antibody engineering : methods and protocols /
Authors: Lo, Benny K. C.
Publisher: Humana Press,
Year: 2004
Subjects: Protein Engineering methods; Antibodies genetics; Antibodies therapeutic use; Immunotechnology Laboratory manuals.; Antibodies therapeutic use Laboratory Manuals.; Antibodies genetics Laboratory Manuals.; Protein Engineering methods Laboratory Manuals.
Contents: pt. 1. Antibody sequence and structure -- Internet resources for the antibody engineer / Benny K.C. Lo -- The Kabat database and a bioinformatics example / George Johnson -- IMGT, the International ImMunoGeneTics Information Sysmtem, http://imgt.cines.fr / Marie-Paule Lefranc -- Antibody variable regions: toward a unified modeling method / Nicholas Whitelegg -- Studying antibody conformations by ultracentrifugation and hydrodynamic modeling / Stephen E. Harding -- pt. 2. Antibody-lead generation -- PCR cloning of human immunoglobulin genes / James D. Marks -- Antibody humanization by CDR grafting / Benny K.C. Lo -- Selection of human antibodies from phage display libraries / James D. Marks -- Production of human single-chain antibodies by ribosome display / Mingyue He -- Production of human antibodies from transgenic mice / C. Geoffrey Davis -- Selection of internalizing antibodies for drug delivery / James D. Marks --^