Title: The baculovirus expression system : a laboratory guide /
Authors: King, L. A. 1959-; Possee, R. D. 1956-
Publisher: Chapman & Hall,
Year: 1992
Subjects: Gene Expression; Baculoviridae genetics; Baculoviruses Genetics Laboratory manuals.; Gene expression Laboratory manuals.
Abstract: A practical guide covering the background, methodological details, applications, advantages and disadvantages of the use of baculovirus expression systems, designed to teach the first-time user the techniques and to highlight more advanced applications for the experienced user.
Contents: The baculoviruses -- The development of baculovirus expression vectors -- Processing of foreign proteins synthesized using baculovirus vectors in insect cells -- Construction of transfer vectors containing the foreign gene -- Insect cell culture media and maintenance of insect cell lines -- Propagation, titration and purification of AcMNPV in cell culture -- Production and selection of recombinant virus -- Characterization of recombinant viruses -- Scaling up the production of recombinant protein in insect cells; laboratory bench level -- Propagation of baculoviruses in insect larvae -- Trouble-shooting guide.