Title: Isotope labeling in biomolecular NMR /
Authors: Atreya, Hanudatta S.
Publisher: Springer,
Year: 2012
Subjects: Isotope Labeling; Nuclear Magnetic Resonance, Biomolecular; Nuclear magnetic resonance spectroscopy.; Radiolabeling.; Biomolecules Analysis.; Isotope Labeling methods; Nuclear Magnetic Resonance, Biomolecular methods
Contents: Proteins -- Isotope labeling methods for large systems -- Segmental labeling to study multidomain proteins -- Isotope labeling for solution and solid-state NMR spectroscopy of membrane proteins -- Isotope labeling methods for relaxation measurements -- Stereo-array isotope labeling method for studying protein structure and dynamics -- Amino acid selective labeling and unlabeling for protein resonance assignments -- Nucleic acids -- Isotope labeling and segmental labeling of larger RNAs for NMR structural studies -- Metabolomics -- Isotope enhanced approaches in metabolomics -- Expression systems -- Cell-free protein synthesis using E. coli cell extract for NMR studies -- Isotope labeling in insect cells -- Mammalian expression of isotopically labeled proteins for NMR spectroscopy.