Title: Subtilisin enzymes : practical protein engineering /
Authors: Bott, Richard; Betzel, Christian.
Publisher: Plenum Press,
Year: 1996
Subjects: Protein Engineering; Subtilisins; Subtilisins Congresses.; Subtilisins Inhibitors Congresses.; Protein engineering Congresses.
Abstract: Presents results from a wide range of disciplines on the most extensively studied model system for protein engineering, not only pointing to lucrative commercial exploitation, but also providing a basis for investigating other members of the subtilase protein superfamily that scientists expect to encounter as genomes are more extensively mapped. The 29 papers cover structural studies of subtilisin enzymes alone and in complex with inhibitors, new enzymes, structure-function studies, biophysical probes and mutagenesis, and protein engineering.
Contents: 1. The Complex between Mesentericopeptidase and Eglin-C / Z. Dauter, C. Betzel, N. Genov and K.S. Wilson -- 2. Crystallographic Study of Eglin-C Binding to Thermitase / A. Teplyakov, P. Gros and W.G.J. Hol -- 3. Design of Specific Peptide Structures and Subtilisin Enzyme Inhibitors Using [alpha], [beta]-Dehydro-Residues / T.P. Singh, B. Padmanabhan, P. Narula, A.K. Saxena, C. Betzel, P. Sharma and S. Dey -- 4. Structure and Function of Subtilisin BPN' as Studied through Crystallographic Studies on a Series of Its Complexes with Genetically Engineered Proteinaceous Inhibitor SSI / T. Nonaka, T. Suzuki, N. Tanaka, S. Saito, T. Senda, K. Miura and Y. Mitsui -- 5. Three Dimensional Structure of the Antibiotic Bacitracin A Complexed to Two Different Subtilisin Proteases: Novel Mode of Enzyme Inhibition / S. Pfeffer-Hennig, Z. Dauter, M. Hennig, W. Hohne, K. Wilson and C. Betzel.