Title: Microarrays in clinical diagnostics /
Authors: Joos, Thomas.; Fortina, Paolo.
Publisher: Humana Press,
Year: 2005
Subjects: DNA microarrays Diagnostic use.; Oligonucleotide Array Sequence Analysis; Diagnostic Techniques and Procedures
Abstract: Leading academic and industrial investigators surveys the world of microarray technology, describing in step-by-step detail diverse DNA and protein assays in clinical laboratories using state-of-the-art technologies. The advanced tools and methods described are designed for mRNA expression analysis, SNP analysis, identification, and quantification of proteins, and for studies of protein-protein interactions. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Contents: Noncontact laser microdissection and pressure catapulting: sample preparation for genomic, transcriptomic and proteomic analysis / Yilmaz Niyaz ... [et al.] -- Applications of the universal DNA microarray in molecular medicine / Reyna Favis ... [et al.] -- Sensitive detection of SARS coronavirus RNA by a novel asymmetric multiplex nested RT-PCR amplification coupled with oligonucleotide microarray hybridization / Zhi-wei Zhang ... [et al.] -- Genotyping single-nucleotide polymorphisms by minisequencing using tag arrays / Lovisa Lovmar and Ann-Christine Syvnen.