Title: Human cell culture protocols /
Authors: Jones, Gareth E., 1947-
Publisher: Humana Press,
Year: 1996
Subjects: Human cell culture Laboratory manuals.; Cells, Cultured; Cytological Techniques
Abstract: In Human Cell Culture Protocols expert scientific and clinical investigators present proven cell culture techniques applicable to tissue samples taken from a wide variety of human organs, particularly those prone to pathological change. They describe in clear, step-by-step instructions the special requirements for successfully culturing such human cells as T-cells, trophoblast cells, renal cells, natural killer cells, endothelial cells, neurons, epithelial cells, pituitary cells, and more. Human Cell Culture Protocols eliminates much of the chore of adapting techniques initially developed for animal cell culture systems, and much of the time consumed in hunting down potentially useful techniques buried in the details of research papers, or even in books largely devoted to animal cell culture. Human Cell Culture Protocols ensures that investigators - whether novice or skilled - can use quickly and successfully high quality, in vitro cultures as models to explore disease processes.
Contents: 1. Establishment and Maintenance of Normal Human Keratinocyte Cultures / Claire Linge -- 2. Cultivation of Normal Human Epidermal Melanocytes / Mei-Yu Hsu and Meenhard Herlyn -- 3. Cultivation of Keratinocytes from the Outer Root Sheath of Human Hair Follicles / Alain Limat and Thomas Hunziker -- 4. Isolation and Short-Term Culture of Human Epidermal Langerhans' Cells / Corinne Moulon -- 5. Growth and Differentiation of Human Adipose Stromal Cells in Culture / Brenda Strutt, Wahid Khalil and Donald Killinger -- 6. Human Fetal Brain Cell Culture / Mark P. Mattson -- 7. Culture of Human Normal Brain and Malignant Brain Tumors for Cellular, Molecular, and Pharmacological Studies / Francis Ali-Osman -- 8. Cell Culture of Human Brain Tumors on Extracellular Matrices: Methodology and Biological Applications / Manfred Westphal, Hildegard Nausch and Dorothea Zirkel -- 9. Isolation and Culture of Human Umbilical Vein Endothelial Cells / David M.L. Morgan.