Title: Gene probes : principles and protocols /
Authors: Aquino de Muro, Marilena.; Rapley, Ralph.
Publisher: Humana Press,
Year: 2002
Subjects: Nucleic Acid Probes; Nucleic acid probes Laboratory manuals.; Nucleic Acid Probes Laboratory Manuals.
Contents: Target format and hybridization conditions -- End labeling procedures: an overview -- Photobiotin labeling -- Nick translation -- Random primed labeling -- Design and evaluation of 16S rRNA-targeted oligonucleotide probes for fluorescence In Situ hybridization -- Evaluation of alternations in the tumor suppressor genes INK4A and INK4B in human bladder tumors -- The [beta]-tubulin gene region as a molecular marker to distinguish Leishmania parasites -- Detection of malignant plasma cells in the bone marrow and peripheral blood of patients with multiple myeloma -- Detection of Chlamydia trachomatis by DNA amplification -- Isolation and identification of Campylobacter species by use of selective enrichment, nucleic acid amplification methods, and gene probes -- Diagnosis of human papillomavirus using In Situ hybridization and In Situ polymerase chain reaction -- Quantitation of In Situ hybridization using image analysis of radioactively labeled RNA probes -- Stable isotope probing for detection of methanotrophs after enrichment with ¹³CH₄ -- Detection of Enterococci in freshwater and seawater (16S and 23S rRNA Enterococci Oligonucleotide Probes) -- Analysis of 16S-23S rRNA intergenic spacer of Vibrio cholerae and Vibrio mimicus for detection of these species -- Colony hybridization of bacterial isolates with Burkholderia cepacia-specific probes -- In Situ hybridization of Burkholderia cepacia cells -- Detection of polychlorinated biphenyl-degrading organisms in soil -- Detection of single-copy genes in DNA from transgenic plants -- Differentiation between transcripts of genes belonging to small families during fruit ripening and abscission -- Polymerase chain reaction detection of nvasive Shigella and Salmonella enterica in food -- Polymerase chain reaction for detection of Listeria monocytogenes.