Title: Protein methods /
Authors: Bollag, Daniel M.; Rozycki, Michael D.; Edelstein, Stuart J.
Publisher: Wiley-Liss,
Year: 1996
Subjects: Chromatography methods; Electrophoresis, Gel, Two-Dimensional methods; Proteins isolation & purification; Proteins isolation & purification.; Proteins analysis; Isoelectric Focusing methods; Immunoblotting methods; Electrophoresis, Gel, Two-Dimensional methods.; Electrophoresis, Agar Gel methods; Proteins Purification.; Proteins Research Methodology.
Abstract: This revised and expanded Second Edition of Protein Methods remains the first source for a complete summary of tested and proven protein techniques. Now divided into two parts, the book begins with the essential chapters from the first edition, updated to reflect important changes in methodology. The chapter on protein isolation includes a new section focusing on the isolation of proteins from inclusion bodies. In the second section, four new chapters are devoted to protein purification and crystallization.
Contents: 1. Preparation for Protein Isolation -- 2. Protein Extraction and Solubilization -- 3. Protein Concentration Determination -- 4. Concentrating Protein Solutions -- 5. Gel Electrophoresis Under Denaturing Conditions -- 6. Gel Electrophoresis Under Nondenaturing Conditions -- 7. Isoelectric Focusing and Two-Dimensional Gel Electrophoresis -- 8. Immunoblotting -- 9. Ion Exchange Chromatography -- 10. Gel Filtration Chromatography -- 11. Affinity Chromatography -- 12. Hanging Drop Crystallization -- Appendix 1: Molecular Weights of Commonly Used Chemicals -- Appendix 2: Molecular Weights and Isoelectric Points of Selected Proteins -- Appendix 3: Ammonium Sulfate Precipitation Table -- Appendix 4: Spectrophotometer Linearity -- Appendix 5: Suppliers and Addresses.