Title: Culture of animal cells : a manual of basic technique /
Authors: Freshney, R. Ian
Publisher: Wiley-Liss,
Year: 2000
Subjects: Tissue Culture Techniques; Cell Culture Techniques; Tissue culture Laboratory manuals.; Cell culture Laboratory manuals.; Tissue culture; Cells, Cultured; Culture Techniques methods; Cell Culture Techniques Laboratory Manuals.; Tissue Culture Techniques Laboratory Manuals.
Abstract: "The Fourth Edition of Culture of Animal Cells: A Manual of Basic Technique offers the most complete training manual of its kind on the fundamental principles and techniques of animal cell culture. Within this volume, indispensable updates reflecting the latest progress in media, specialized techniques, biotechnology, DNA transfer, and tumor culture have been made. This edition has five new chapters expanding on serum-free media, scale-up and biofermentors, molecular techniques, immortalization, and troubleshooting." "Biomedical researchers in cell biology, cytology, molecular biology, immunology, neuroscience, toxicology, and cancer biology will find Culture of Animal Cells: A Manual of Basic Technique, Fourth Edition to be an invaluable reference."--Jacket.
Contents: 1. Introduction -- 2. Biology of Cultured Cells -- 3. Design and Layout -- 4. Equipment -- 5. Aseptic Technique -- 6. Safety -- 7. Culture Vessels -- 8. Media -- 9. Serum-Free Media -- 10. Preparation and Sterilization -- 11. Primary Culture -- 12. Cell Lines -- 13. Cloning and Selection -- 14. Cell Separation -- 15. Characterization -- 16. Differentiation -- 17. Transformation -- 18. Contamination -- 19. Cryopreservation -- 20. Quantitation -- 21. Cytotoxicity -- 22. Specialized Cells -- 23. Tumor Cells -- 24. Organotypic Culture -- 25. Scale-Up -- 26. Specialized Techniques -- 27. Molecular Techniques -- 28. Problem Solving.