Title: Two-hybrid systems : methods and protocols /
Authors: MacDonald, Paul N.
Publisher: Humana Press,
Year: 2001
Subjects: Plasmids; Yeasts; Protein Binding.; Plasmids.; Protein binding Research Methodology.; Yeast fungi.; Two-Hybrid System Techniques; Yeasts genetics
Contents: Part I. General Methods -- The Two-Hybrid System : A Personal View / Stanley Fields and Paul L. Bartel -- Growth and Maintenance of Yeast / Lawrence W. Bergman -- Media Formulations for Various Two-Hybrid Systems / Michael Saghbini, Denise Hoekstra, and Jim Gautsch -- Yeast Two-Hybrid Vectors and Strains / Philip James -- High-Efficiency Transformation of Plasmid DNA into Yeast / Robin A. Woods and R. Daniel Gietz -- Qualitative and Quantitative Assessment of Interactions / Monica M. Montano -- Strategies for Rescuing Plasmid DNA from Yeast Two-Hybrid Colonies / Alyson Byrd and René St-Arnaud -- Part II. False Positives -- Two-Hybrid System and False Positives : Approaches to Detection and Elimination / Ilya G. Serebriiskii and Erica A. Golemis -- Confirming Yeast Two-Hybrid Protein Interactions Using In Vitro Glutathione-S-Transferase Pulldowns / Dennis M. Kraichely and Paul N. MacDonald --^