Category: 基因功能实验观察
Question: What methods were used in the research on the role of OsSIZ2 in rice development and Pi homeostasis?
Answer: The methods used in the research included the generation of T-DNA insertion knockout and RNA interference-mediated knockdown mutants, two-round PCR for screening putative T-DNA insertion mutants, semi-quantitative RT-PCR for validating the lack of OsSIZ2 transcripts in ossiz2 mutants, qRT-PCR for determining the relative expression levels of OsSIZ2 in wild-types and the mutants, and Southern blot analysis for confirming a single T-DNA insertion in ossiz2.