Category: 基因功能实验观察
Question: What were the results of the study on RFT1?
Answer: Among 30 independently transformed calli, three regenerated leaves and plantlets but did not proceed to mature vegetative stages. These three produced small panicles with only a few spikelets. qRT-PCR analyses showed RFT1 was expressed in all three transgenic plants, indicating the early flowering phenotypes were due to its overexpression. Overexpression of RFT1 resulted in the direct formation of spikelets from most of the transgenic calli. Transcript analyses showed that expression of OsMADS14, OsMADS15, OsMADS18, and OsMADS34 in those calli was strongly induced during the reproductive phase.