I list the peer reviewed publications that support the information that the use CRUSIO continues to remove is shown below The name of the system that was developed in Dr Timiras lab is called the Automated Imaging Microscope System and not the automated tissue image analysis system that the user CRUSIO continues to call it Please refer to the PUBMED publication at to verify the name in the 4th sentence of the Abstract Please verify the information related to CREA at the CREA web site and I would be very happy to discuss these references with you and information with you Estrogen receptor alpha immunoreactivity in the arcuate hypothalamus of young and middle aged female mice Yaghmaie F Saeed O Garan SA Voelker MA Sternberg H Timiras PS Neuro Endocrinol Lett 2010 Feb 16 31 1 15 Age dependent loss of insulin like growth factor 1 receptor immunoreactive cells in the supraoptic hypothalamus is reduced in calorically restricted mice Yaghmaie F Saeed O Garan SA Voelker MA Gouw AM Freitag W Sternberg H Timiras PS Neurology Neurosurgery Para 488 Page 99 Sec 8 Vol 152 2 2007 Tracking changes in hypothalamic IGF 1 sensitivity with aging and caloric restriction F Yaghmaie O Saeed S A Garan A M Gouw P Jafar J Kaur S Nijjar P S Timiras H Sternberg and M A Voelker Experimental Gerontology Volume 42 Issues 1 2 January February 2007 Pages 11 12 Insulin like growth factor 1 receptor immunoreactive cells are selectively maintained in the paraventricular hypothalamus of calorically restricted mice Saeed O Yaghmaie F Garan SA Gouw AM Voelker MA Sternberg H Timiras PS Int J Dev Neurosci 2007 Feb 25 1 23 8 Age dependent loss of insulin like growth factor 1 receptor immunoreactive cells in the supraoptic hypothalamus is reduced in calorically restricted mice Yaghmaie F Saeed O Garan SA Voelker MA Gouw AM Freitag W Sternberg H Timiras PS Int J Dev Neurosci 2006 Nov 24 7 431 6 A study of insulin like growth factor I receptor immunoreactivity in the supraoptic nucleus of young and old female B6D2F1 mice Farzin Yaghmaie Omar Saeed Steven A Garan Arvin M Gouw Tien Tran Jacqueline Ho Liu Y Zhao Mark A Voelker and Paola S Timiras FASEB Journal April 2006 357 5 Caloric restriction reduces cell loss and maintains estrogen receptor alpha immunoreactivity in the pre optic hypothalamus of female B6D2F1 mice Yaghmaie F Saeed O Garan SA Freitag W Timiras PS Sternberg H Neuro Endocrinol Lett 2005 Jun 26 3 197 203 A survey of estrogen receptor alpha immunoreactivity in the hypothalamus of young old and old calorie restricted female B6D2F1 mice F Yaghmaie S A Garan O Saeed W Freitag P S Timiras H Sternberg M Voelker Experimental Gerontology Volume 39 Issues 11 12 November December 2004 Page 1771 A comparison of estrogen receptor alpha immunoreactivity in the arcuate hypothalamus of young and middle aged C57BL6 female mice Yaghmaie F Garan SA Massaro M Timiras PS Experimental Gerontology Volume 38 2003 Issues 1 2 Page 220 Creating Three Dimensional Neuronal Maps of the Mouse Hypothalamus Using an Automated Imaging Microscope System Garan SA Neudorf J Tonkin J McCook LR Timiras PS Experimental Gerontology Volume 35 numbers 9 10 December 2000 page 1421 Automated Imaging Microscope System Jason Neudorf and Steven A Garan Linux Journal 2000 Issue 70 Februrary Page 32 35