I was thinking of editing this stub to use simpler language and explain exactly what a daisy chain of DNA is The change I want to make is for the article to say this Daisy chaining DNA is the process of when DNA undergoing PCR becomes tangled and forms a daisy chain During PCR primers or dNTP s will eventually be used up and limit further reactions it is when the primers deplete that causes daisy chaining Since the denaturing and annealing processes still continue without primers the single stranded DNA molecules reanneal to themselves However this reannealing does not always occur to another complementary strand It is this imperfect match up that causes tangles These tangles look like a daisy chain I used this source and will of course site it Thoughts