the advent of combination antiretroviral therapy ( art ) has increased life expectancy and decreased severe forms of hiv - associated dementia and other co - morbidities in most hiv - infected individuals . however , milder forms of hiv - associated neurocognitive disorders are still estimated to affect one - third to one - half of successfully treated patients . neurocognitive impairment has been associated with residual immune dysfunction , which persists in some individuals despite long term suppressive art . the exact mechanism of chronic immune dysfunction in these individuals is incompletely understood and most likely multifactorial . translocation of microbial products from the gastrointestinal tract into the systemic circulation is likely an important driver of immune dysfunction and persistent inflammation during suppressive art . it may also play a role in the pathogenesis of neurocognitive dysfunction during hiv - infection . ( 13)--d - glucan ( bdg ) is a polysaccharide cell wall component of most fungal species including candida spp . blood and cerebrospinal fluid ( csf ) bdg levels are useful for early diagnosis of invasive fungal infections . in the absence of an active invasive fungal infection , increased blood bdg levels may be an indicator of gut mucosal barrier disruption and microbial translocation . the objective of this study was to evaluate whether higher blood bdg levels correlate with impaired neurocognitive functioning ( evaluated by global deficit score [ gds ] ) in a cohort of adults with acute / early hiv infection , who initiated art during the earliest phase of infection and achieved suppressed levels of hiv rna in blood plasma thereafter . in this cross - sectional cohort study , we measured levels of bdg in blood plasma and csf supernatant samples in a cohort of adults with suppressed levels of hiv rna in blood plasma ( < 50 copies / ml ) , and compared them with established biomarkers of microbial translocation , immune activation , and cognitive dysfunction . all 21 subjects participated in the san diego primary hiv infection research consortium ( sd pirc ) , which comprised of adults with acute or early hiv diagnosis , early art initiation , and suppressed levels of hiv rna in blood plasma throughout treatment . study samples were collected prospectively as part of the sd pirc between december 2013 and june 2014 at the university of california , san diego , and stored at 80  c on the day of collection . degree of neurocognitive impairment at the time of sample collection was measured using the global deficit score ( gds ) , which is an established and sensitive method to determine neurocognitive functioning among individuals living with hiv . the gds has been shown to detect mild , hiv - associated cognitive impairment based on assessment of multiple cognitive domains . briefly , individuals completed a comprehensive neuropsychological test battery consistent with frascati recommendations for neuroaids research . raw scores were converted to demographically adjusted t - scores , which , in turn , were scaled to deficit scores ranging from 0 ( normal , t  >  39 ) to 5 ( severely impaired , t  <  20 ) . nineteen plasma and 16 csf supernatant samples ( paired same - day plasma and csf samples were available from 14/21 subjects , plasma samples only from 5/21 subjects , and csf samples only from 2/21 subjects ) were retrospectively evaluated for bdg levels and established biomarkers of microbial translocation and immune activation . bdg levels were measured using the fungitell assay in june 2015 at the associates of cape cod , inc , research laboratories ( associates of cape cod , inc , east falmouth , ma ) . soluble cluster of differentiation 14 ( scd14 ) levels were measured by an enzyme - linked immunosorbent assay ( elisa ; r&d systems inc , minneapolis , mn ) . interleukin ( il ) 8 levels were measured by an electrochemiluminescence multiplex assay ( meso scale diagnostics , rockville , md ) , each according to the manufacturer 's procedures . for statistical analysis , spss 21 ( spss inc , chicago , il ) was used . correlation between levels of bdg , gds , and levels of other biomarkers were calculated using spearman correlation analysis because of the non - normal distributions of gds scores and other biomarkers . gds scores were also squareroot - transformed to achieve a distribution close to normal to allow for additional pearson correlation analysis between normally distributed biomarkers and squareroot - transformed gds . power analysis revealed that a sample size of 19 plasma samples provides at least 80% power ( with alpha  =  0.05 ) to detect a correlation of r  =  0.351 or higher for correlations of 2 variables . the ucsd human research protections program approved the study protocol , consent , and all study - related procedures . all study participants provided voluntary , written informed consent before any study procedures were undertaken . all 21 subjects participated in the san diego primary hiv infection research consortium ( sd pirc ) , which comprised of adults with acute or early hiv diagnosis , early art initiation , and suppressed levels of hiv rna in blood plasma throughout treatment . study samples were collected prospectively as part of the sd pirc between december 2013 and june 2014 at the university of california , san diego , and stored at 80  c on the day of collection . degree of neurocognitive impairment at the time of sample collection was measured using the global deficit score ( gds ) , which is an established and sensitive method to determine neurocognitive functioning among individuals living with hiv . the gds has been shown to detect mild , hiv - associated cognitive impairment based on assessment of multiple cognitive domains . briefly , individuals completed a comprehensive neuropsychological test battery consistent with frascati recommendations for neuroaids research . raw scores were converted to demographically adjusted t - scores , which , in turn , were scaled to deficit scores ranging from 0 ( normal , t  >  39 ) to 5 ( severely impaired , t  <  20 ) . nineteen plasma and 16 csf supernatant samples ( paired same - day plasma and csf samples were available from 14/21 subjects , plasma samples only from 5/21 subjects , and csf samples only from 2/21 subjects ) were retrospectively evaluated for bdg levels and established biomarkers of microbial translocation and immune activation . bdg levels were measured using the fungitell assay in june 2015 at the associates of cape cod , inc , research laboratories ( associates of cape cod , inc , east falmouth , ma ) . soluble cluster of differentiation 14 ( scd14 ) levels were measured by an enzyme - linked immunosorbent assay ( elisa ; r&d systems inc , minneapolis , mn ) . interleukin ( il ) 8 levels were measured by an electrochemiluminescence multiplex assay ( meso scale diagnostics , rockville , md ) , each according to the manufacturer 's procedures . for statistical analysis , spss 21 ( spss inc , chicago , il ) was used . correlation between levels of bdg , gds , and levels of other biomarkers were calculated using spearman correlation analysis because of the non - normal distributions of gds scores and other biomarkers . gds scores were also squareroot - transformed to achieve a distribution close to normal to allow for additional pearson correlation analysis between normally distributed biomarkers and squareroot - transformed gds . power analysis revealed that a sample size of 19 plasma samples provides at least 80% power ( with alpha  =  0.05 ) to detect a correlation of r  =  0.351 or higher for correlations of 2 variables . the ucsd human research protections program approved the study protocol , consent , and all study - related procedures . all study participants provided voluntary , written informed consent before any study procedures were undertaken . the study cohort was composed of 21 hiv - infected participants without symptoms of opportunistic infections . demographics and clinical characteristics at the time of sample collection are depicted in table 1 . all participants were virologically suppressed at the time of sampling in both compartments ( blood and csf ) . the median cns penetration effectiveness index was 6 ( iqr 47 ) for their current art regimens . no correlations were found between blood bdg levels and age , sex , and estimated duration of infection . demographics and clinical characteristics of the study population median gds was 0.39 ( range 02.67 ; iqr 0.111.11 ; 10 subjects [ including one subject with csf sample only ] had gds > 0.5 , which is considered at least mild cognitive impairment ) . median bdg level in blood plasma was 66  pg / ml ( range : 20101  pg / ml ) , whereas median bdg level in csf supernatant was 5  pg / ml ( range : 053  pg / ml ) . higher levels of plasma bdg were associated with more severe cognitive impairment as measured by the gds ( spearman r  =  0.47 , p  =  0.042 ; pearson [ correlation with squareroot - transformed gds ] r  =  0.46 , p  =  0.049 ; figure 1 and table 2 ] . of the other biomarkers , a significant correlation with gds was found for il-8 ( spearman r  =  0.55 ; p  =  0.014 ) , whereas no significant correlations were found for scd14 ( spearman r  =  0.4 , n.s . ) , and nadir cd4 count ( spearman r  =  0.01 , n.s . ) . also , we found no significant correlation between plasma bdg and both il-8 ( spearman r  =  0.12 , n.s . ) and scd14 ( spearman r  =  0.38 , n.s . ) . scatterplots of correlation of blood beta - d - glucan levels ( a ) and cerebrospinal fluid beta - d - glucan levels ( b ) with global deficit scores . levels of investigated plasma biomarkers ( median and iqr are displayed ) and correlations with gds and plasma bdg levels with regard to combinations of bdg , il-8 , and scd14 and correlation with gds , bdg  +  il-8 ( spearman r  =  0.57 ; p  =  0.01 ) and bdg  +  scd14 ( spearman r  =  0.58 , p  <  0.01 ) were the most promising combinations of 2 biomarkers , whereas the combination of all 3 biomarkers ( bdg  +  il-8  +  scd14 ) had the highest correlation with gds ( spearman r  =  0.71 ; p  <  0.01 ) . plasma bdg levels , levels of other biomarkers including combinations , as well as spearman correlations with blood bdg and gds are displayed in table 2 . using the plasma bdg cut - off recommended by the manufacturer for differentiating negative from intermediate and positive results ( ie of 60  pg / ml ) , the sensitivity for detecting neurocognitive impairment was 78% and the specificity 50% . if bdg cut - off was increased to 70  pg / ml , the sensitivity dropped to 44% , but specificity increased to 90% . two of 16 csf samples presented elevated bdg levels ( 45 and 53  pg / ml ; figure 1 ) , whereas all other samples had bdg levels < 10  pg / ml . interestingly , these 2 samples originated for the 2 subjects with the highest gds scores of the cohort . both of these subjects had also elevated serum bdg levels ( 4 and 5 highest of the study cohort ) and no signs or symptoms of opportunistic infections . this study correlated blood levels of the fungal polysaccharide cell wall component bdg , with cognition among hiv - infected patients . identifying biomarkers associated with worse neurocognitive functioning among successfully treated hiv - infected individual overall , our study cohort of hiv - infected individuals with suppressed hiv rna viral loads presented with markedly higher bdg levels when compared to previously published levels from healthy patients undergoing elective plastic surgery procedures . our study also showed , for the first time , that elevated bdg levels in the absence of fungal infections were associated with negative neurocognitive outcomes ( as measured by increased gds ) in virologically suppressed hiv - infected persons who started art during early hiv infection . interestingly , gds was also correlated with il-8 , but bdg and il-8 were not correlated . importantly , combining bdg with il-8 and especially the combination of all 3 plasma biomarkers ( bdg , il-8 , and scd 14 ) further improved the correlation with gds . we hypothesize that elevated plasma bdg may primarily reflect translocation of products from natural fungal flora from the gastrointestinal tract into systemic circulation . evidence of peripheral fungal cell wall polysaccharides in the systemic circulation has also been reported previously in an hiv - infected outpatient cohort , the majority of whom had measurable hiv rna in their blood . in that study , high serum bdg was associated with hiv - associated immunosuppression ( ie , cd4 cell counts < 200  cells/l ) , inflammation ( correlation with plasma il-8 and other inflammation markers ) , and cardiopulmonary comorbidity . together with these previous findings , our results support a theory that bdg translocation occurs in virologically suppressed hiv+ individuals and may relate to important outcomes such as nc impairment . bdg levels were markedly higher ( mean 142  pg / ml ) in a hiv - infected cohort with lower median cd4 counts ( 26 , iqr 1053 , all without opportunistic infections ) , when compared with the cohort studied here ( with a median cd4 count > 700  pg / ml ) . in contrast , lower bdg levels ( median 15  pg / ml ) were found in another study that evaluated chronically hiv - infected individuals with a median duration of hiv infection of 15 years and high cd4 + counts ( 643 , range 1961740 ) . although elevated blood bdg levels may be associated with microbial translocation in all hiv - infected individuals ( ie , independent of cd4 cell counts ) , interpretation of elevated blood bdg levels in patients with cd4 cell counts below 200 to 300  cells/l may be more complicated . although it seems intuitive that deteriorating cd4 counts are associated with worse mucosal barrier function , other reasons for elevated bdg may include potential colonization or subclinical infection with candida spp or pneumocystis that may occur more frequently in individuals with lower cd4 counts . other studies suggested that bdg may also be elevated in bloodstream infections caused by primarily enteric bacteria , such as enterococcus spp , whereas in a recent study , bdg was not elevated ( i.e. >  80  pg / ml ) in patients with mild - to - moderate mucositis . whether this increase in bdg levels reflects concomitant translocation of fungal elements , major limitations of this pilot study include the small sample size , that it is single - site , cross - sectional , and restricted to patients treated in early stages of hiv infection . to further examine the role of bdg as a potential biomarker for translocation of gut luminal contents and its correlation with neurocognitive impairment bdg levels in the intestinal luminal contents are also likely to be highly variable on an individual basis , and a standardized oral bdg challenge approach may be more suitable for assessing gut leakiness . longitudinal studies are also needed to investigate the mechanisms of blood bdg increase in individuals with translocation of gut microbes or their components . in conclusion , bdg may be an indicator of gut mucosal barrier disruption and an independent biomarker associated with neurocognitive functioning in virologically suppressed hiv - infected individuals with high cd4 counts . in particular , when bdg is combined with established markers of immune activation , diagnostic potential for neurocognitive functioning may be promising .
abstractmicrobial translocation from the gut is associated with immune dysfunction , persistent inflammation , and likely plays a role in the pathogenesis of neurocognitive dysfunction during hiv infection . 
 ( 13)--d - glucan ( bdg ) is a component of most fungal cell walls and might be a useful indicator of gut mucosal barrier impairment . 
 the objective of this study was to evaluate whether higher blood bdg levels correlate with impaired neurocognitive functioning in a cohort of hiv - infected adults with suppressed levels of hiv rna in blood plasma.in this cross - sectional cohort study , we measured levels of bdg in blood plasma and cerebrospinal fluid ( csf ) supernatant samples in a cohort of adults with acute / early hiv infection , who initiated antiretroviral therapy ( art ) during the earliest phase of infection and achieved suppressed levels of hiv rna in blood plasma ( < 50 copies / ml ) thereafter . 
 we compared bdg with established biomarkers of microbial translocation , immune activation , and cognitive dysfunction ( evaluated by global deficit score).we found that higher blood bdg levels were significantly related to higher global deficit scores , reflecting worse neurocognitive performance ( spearman r  =  0.47 ; p  =  0.042 ) among hiv - infected adults with suppressed viral loads who initiated art early in infection . 
 two csf samples presented elevated bdg levels . 
 interestingly , these 2 samples originated from the 2 subjects with the highest global deficit scores of the cohort.bdg may be a promising independent biomarker associated with neurocognitive functioning in virologically suppressed hiv - infected individuals .