children with type 1 diabetes of at least 1 year duration , attending diabetes clinics at children s hospital new orleans , were invited to participate . data regarding duration of diabetes , height , weight , date of birth , race , and sex were collected at the time of visit , and additional data regarding prior hba1c and mbg levels were extracted from patient medical records . the study was approved by the institutional review board at louisiana state university health sciences center and the children s hospital new orleans . at the time of clinic visit , the patient s glucose meter was inspected for proper operation . if no problems were found , the mbg from the 30 days prior to the visit was calculated and recorded . a blood sample at the time of the clinic visit was obtained for hba1c and glucose ( blood glucose at time of clinic visit [ cbg ] , measured by accu - chek inform , model 2001201 , roche diagnostics , indianapolis , in ) . hgi at the time of the clinic visit was calculated as the difference between a patient s observed hba1c minus the hba1c level predicted from the patient s observed mbg based on the population regression of hba1c on mbg ( predicted hba1c = 0.021  mbg + 4.3 ) ( 16 ) . we have previously shown the intra- and interindividual consistency over time of hba1c , mbg , and hgi collected from clinic data ( 16 ) . during one clinic visit , sif levels were noninvasively measured from the volar surface of the left forearm from each subject using a scout ds instrument ( veralight , inc . ) . the device sequentially excited the skin surface using different light - emitting diodes ( leds ) that had peak excitation wavelengths of 375 , 405 , and 420 nm . skin reflectance was measured for each excitation led , and a white light led was used to measure skin reflectance over the emission region . spectral data collected by the instrument were transmitted directly to veralight , inc . for further analysis to adjust for the impact of skin pigmentation , hemoglobin content , light scattering , and other dermal characteristics . data were adjusted mathematically using two different kx / km parameter sets on the measured reflectance at the excitation and emission wavelengths . for the first kx / km set ( designated as set u ) , kx and km were 1.0 and 0.0 , respectively . for the second kx / km set ( set c ) , kx and km were 0.5 and 0 , respectively , which is useful for populations with a wide range of skin melanin ( 17 ) ( further technical detail in supplementary appendix ) . sif data are reported in arbitrary relative fluorescence units as a function of excitation wavelength and which kx / km adjustment (  u  or  c  ) used . data were analyzed using hba1c , mbg , hgi , and cbg at the time of the visit when sif was measured . in addition , the means of mbg , hba1c , and hgi from all available prior clinic visits for the patient were also evaluated . as results were similar whether using data for that clinic visit or the mean of data from all available clinic visits , we report here the mean data . the influence of biological variation in hba1c on sif was evaluated in multivariate regression models using either hgi or by substituting hba1c adjusted for mbg in the model . for evaluation purposes , cbg was substituted in the models for mbg for single clinic visits . models were further adjusted for chronological age , duration of diabetes , sex , race , and bmi z - score ( z - bmi ) using the glm procedure in sas software ( sas institute , cary , nc ) . children with type 1 diabetes of at least 1 year duration , attending diabetes clinics at children s hospital new orleans , were invited to participate . data regarding duration of diabetes , height , weight , date of birth , race , and sex were collected at the time of visit , and additional data regarding prior hba1c and mbg levels were extracted from patient medical records . the study was approved by the institutional review board at louisiana state university health sciences center and the children s hospital new orleans . at the time of clinic visit , the patient s glucose meter was inspected for proper operation . if no problems were found , the mbg from the 30 days prior to the visit was calculated and recorded . a blood sample at the time of the clinic visit was obtained for hba1c and glucose ( blood glucose at time of clinic visit [ cbg ] , measured by accu - chek inform , model 2001201 , roche diagnostics , indianapolis , in ) . hgi at the time of the clinic visit was calculated as the difference between a patient s observed hba1c minus the hba1c level predicted from the patient s observed mbg based on the population regression of hba1c on mbg ( predicted hba1c = 0.021  mbg + 4.3 ) ( 16 ) . we have previously shown the intra- and interindividual consistency over time of hba1c , mbg , and hgi collected from clinic data ( 16 ) . during one clinic visit , sif levels were noninvasively measured from the volar surface of the left forearm from each subject using a scout ds instrument ( veralight , inc . ) . the device sequentially excited the skin surface using different light - emitting diodes ( leds ) that had peak excitation wavelengths of 375 , 405 , and 420 nm . skin reflectance was measured for each excitation led , and a white light led was used to measure skin reflectance over the emission region . spectral data collected by the instrument were transmitted directly to veralight , inc . for further analysis to adjust for the impact of skin pigmentation , hemoglobin content , light scattering , and other dermal characteristics . data were adjusted mathematically using two different kx / km parameter sets on the measured reflectance at the excitation and emission wavelengths . for the first kx / km set ( designated as set u ) , kx and km were 1.0 and 0.0 , respectively . for the second kx / km set ( set c ) , kx and km were 0.5 and 0 , respectively , which is useful for populations with a wide range of skin melanin ( 17 ) ( further technical detail in supplementary appendix ) . sif data are reported in arbitrary relative fluorescence units as a function of excitation wavelength and which kx / km adjustment (  u  or  c  ) used . data were analyzed using hba1c , mbg , hgi , and cbg at the time of the visit when sif was measured . in addition , the means of mbg , hba1c , and hgi from all available prior clinic visits for the patient were also evaluated . as results were similar whether using data for that clinic visit or the mean of data from all available clinic visits , we report here the mean data . the influence of biological variation in hba1c on sif was evaluated in multivariate regression models using either hgi or by substituting hba1c adjusted for mbg in the model . for evaluation purposes , models were further adjusted for chronological age , duration of diabetes , sex , race , and bmi z - score ( z - bmi ) using the glm procedure in sas software ( sas institute , cary , nc ) . characteristics of the patient population studied at the time of sif measurement in clinic are presented in table 1 . in the patient population , mean hba1c ( mhba1c ) was correlated with mean mbg ( mmbg ) ( r = 0.5 ; p < 0.001 ) but not the visit cbg ( r = 0.07 ; p = 0.51 ) . mhba1c was correlated with mean hgi ( mhgi ) ( r = 0.8 ; p < 0.001 ) . the sif data for the three excitation wavelengths were highly intercorrelated within the same adjustment set whether  u  or however , correlations between  u  and  c  data were considerably lower ( supplementary table 3 ) . patient characteristics at time of clinic visit ( n = 110 ) results from the single visit data were similar to the results using mean values from multiple visits . simple correlation analyses were performed between  u  and  c  adjustments of sif with mmbg , mhba1c , mhgi , age , and duration of diabetes ( table 2 ) . mmbg , cbg , and z - bmi were not correlated with any of the sif data . pearson correlation between  u  and  c  set sif adjustments with mmbg , mhba1c , mhgi , age , and duration of diabetes the top number in each cell is the correlation coefficient ( r ) , and the bottom number is the significance level ( p value ) ; n = 110 . multivariate regression analysis showed that mhba1c adjusted for mmbg , or mhgi substituted in the model for mhba1c , was consistently associated with all sif excitation wavelengths , both u  and  c  , after also controlling for presence of age , race , sex , z - bmi , and duration of diabetes ( table 3 ) . mmbg and z - bmi were not statistically significant in relationship with any of the sif measures . multivariate regression analysis between  u- and  c-adjusted sif levels at each wavelength as dependent variables with patient age , duration of diabetes ( dod ) , sex , race , z - bmi , hba1c or hgi , and mbg statistically significant relationships , p = 0.05 or less , for the covariates in the model are indicated by x. the relationship between 405-nm excited sif using set age , duration of diabetes , and sex were statistically significant covariates for the  c  sif data at all three wavelengths . sif levels increased with age and were higher in girls than boys at any given age ( fig . race was a significant covariate for all sif excitation wavelengths with the  u  adjustment , but only for 375 nm in the  c-adjusted sif data , blacks being higher than whites . to our knowledge this is the first in - depth study of the relationship of skin ages ( sages ) with hba1c , mbg , hgi , and other characteristics in a biracial population of children with type 1 diabetes . we used a novel technology to estimate sages by measuring sif using a scout ds device at three different excitation wavelengths and two mathematical adjustment sets . prior reports using this device have focused on its ability to identify adults with abnormalities of glucose tolerance ( 13,14 ) and the relationship of sif with coronary artery calcifications in type 1 diabetes ( 12 ) . spectral information read by the scout device from the skin is a combination of reflected light and fluorescence excited in the tissue by leds . because of variability in skin pigmentation , skin thickness , hemoglobin , and other factors , the excitation and emission data returning to the device must be adjusted to minimize the distortion of the skin fluorescence by the aforementioned factors . u  and  c  adjustments of the sif data ( table 3 ) . with the  u  adjustment , all data from the three excitation sif wavelengths evaluated were consistently higher in black than in white patients , consistent with differences in skin pigmentations . such a difference was only found at the 375-nm sif excitation wavelength with the  c  adjustment . sages and auto - fluorescence have been shown to increase with aging and duration of diabetes in dermal biopsy samples from patients with diabetes ( 15,18,19 ) . however , the  u  adjustment from our sif data was insensitive to differences related to duration of diabetes and less sensitive to age - related change . our finding of a significant increase in  c-adjusted sif at all excitation wavelengths with patient age and duration of diabetes in children is in accordance with prior biopsy data , albeit from adults . c-adjusted sif levels between girls and boys that was independent of age , mbg , or hba1c level . there was no sex difference discernable using the  u  adjustment , which agrees with reported findings in adults using a similar method ( 20 ) , with the exception of smokers ( 21 ) . potentially the detected sex difference in sif might represent a biochemical difference in accumulation of fluorescent ages between the sexes during childhood and adolescence . alternatively this difference may represent non - age differences and be an artifact of the adjustment method or other factors . it is conventionally understood that formation and accumulation of glycated proteins such as hba1c ( 4 ) and ages ( 2 ) is a concentration - dependent function of glucose concentration . the sif data demonstrated a strong relationship with hba1c or hgi whether  u  or previous investigators have studied skin autofluorescence during oral glucose tolerance testing and noted no changes related to acute fluctuations in blood glucose ( 23 ) . initial nonenzymatic attachment of glucose to a protein such as hemoglobin occurs quickly and reversibly , but the formation of the stable amadori product takes much longer , and progression to ages even more time . thus a randomly obtained glucose level , while correlated with the labile schiff base precursor to hba1c , is negligibly associated with stable hba1c levels ( 24 ) . the lack of association of sif with mbg is more surprising . both mbg and hba1c levels have been associated with the development of complications in type 1 diabetes ( 6 ) . hba1c is strongly correlated with mbg and widely used to estimate mbg ( 25 ) . however in simple pearson correlation analysis , hba1c and hgi were correlated with the sif levels whereas mbg was not . in the multivariate models , both hba1c and hgi were significantly related to sif at that visit , but the visit mbg as a covariate was not . considering the possibility that the 30-day average mbg from the clinic visit might have been insufficiently long to be involved with detectable age formation , we substituted the average of all available prior mbg measurements from patients into the model ( along with hgi or hba1c ) . these findings suggest that mbg - independent , between - patient differences in hba1c are predictive of age burden in the skin and potentially influence the development of diabetes complications . thus factors besides mbg that can influence nonenzymatic glycation ( 24 ) may be more influential in formation of skin ages and development of diabetes complications than just mbg exposure alone .
objectiveto evaluate the relationship between skin advanced glycation end products ( sages ) with mean blood glucose ( mbg ) , hemoglobin a1c ( hba1c ) , and mbg - independent , between - patient differences in hba1c among children with type 1 diabetes.research design and methodschildren aged 5 to 20 years with type 1 diabetes of at least 1 year duration participated . at a clinic visit , 
 sage was estimated noninvasively by measurement of skin intrinsic fluorescence ( sif ) . 
 sif data were adjusted to correct for variation in skin pigmentation . 
 mbg - independent , between - patient differences in hba1c were examined by statistically controlling hba1c for mbg or alternatively by use of a hemoglobin glycation index ( hgi ) . 
 results were similar whether hba1c , mbg , and hgi were analyzed as single values from the time of the sif examination visit or as the mean values from all available visits of the patient.resultshba1c was correlated with mbg ( r = 0.5 ; p < 0.001 ; n = 110 ) . 
 hba1c and hgi , but not mbg , were statistically associated with sif after adjustment for age , duration of diabetes , race , sex , and bmi z - score . 
 sif increased with age and duration of diabetes and was higher in girls than boys.conclusionssage levels estimated by sif increase with age , duration of diabetes , and female sex . 
 sage is correlated with mbg - independent biological variation in hba1c , but not with mbg itself . 
 these results suggest that factors besides mbg that influence hba1c levels also contribute to accumulation of sage .