eligibility requirements included body mass index > 18.5 kg / m ( mean 24.8sd 4.5 kg / m ) , age 1850 years ( 267.5 ) , no current use of antioxidant dietary supplements or a willingness to refrain from their use three weeks prior to and during the study , and self - reported smoking of 3 cigarettes / day ( 11.05.1 ) for 1 year ( 8.06.3 ) . smoking status was confirmed by a saliva sample analyzed for cotinine by salimetrics , llc , state college , pa ( 189.3121.0 ng / ml ) . ( 21 ) , was 50.528.2 m , which was between the 59.1 m detected in passive smokers and the 40.3 m detected in persons smoking 15 cigarettes per day ( 22 ) . half of the participants consumed gj in the first experimental session and placebo in the second ; the other half followed the opposite order . most participants three sessions were on the same day of the week over the course of three consecutive weeks . each session consisted of two visits to the laboratory  a lunch visit and a diet recall visit the following day . the treatments were 10 ml / kg body weight of a placebo beverage or 100% concord gj containing 2,100 mg / l total phenolics as gallic acid equivalents ( beverages and data supplied by welch foods , inc . the placebo was formulated to match concord gj for energy , fructose and glucose content , acidity , taste , color and aroma . participants were instructed to eat a similar breakfast on each treatment day ( which participants reported during the following day 's diet recall ) and not to consume any food or energy - containing beverage within three hours of arrival at the laboratory . participants were also instructed not to consume any additional gj or red wine for the entire day . for the lunch visit , participants arrived at the laboratory between 12:00 and 13:00 hours on weekdays and completed a profile of mood states questionnaire ( poms ) ( multi - health systems inc . , north tonawanda , ny ) and an affect grid ( ag ) ( 23 ) . next , participants completed the first of four word fragment completion ( wfc ) tasks ( see description below ) , followed by an appetite assessment . appetite ratings were provided on a personal digital assistant ( pda ) programmed to record marks made by participants on visual analog scales . next , participants had up to 30 minutes to consume a lunch of as much kraft easy mac ( kraft foods inc . , northfield , il ) as desired , one 99 g hunt 's snack pack butterscotch pudding cup ( conagra foods inc . , omaha , ne ) , and the treatment beverage . after lunch , participants provided appetite ratings and completed a second poms and ag . then , they performed a second wfc task . immediately afterward , another ag was completed , followed by a third wfc task and another ag . finally , a fourth and final wfc task was completed , followed by a third set of appetite ratings , the final poms and the final ag . during all wfc tasks , participants wore noise - reducing headphones to reduce auditory distractions ( maxell noise cancellation headphone , maxell corporation of america , fair lawn , nj ) . after leaving the laboratory , participants provided hourly appetite ratings on the pdas and continued recording their food intake for the remainder of the day . the following day , participants returned their pda to the laboratory and participated in a computer - aided 24-hour diet recall interview with nutrition data system for research software ( nds - r , 2005 and ndsr , 2006 nutrition coordinating center , university of minnesota , minneapolis , mn ) . due to the nature of a within - subject study design and the desire not to arouse participants out of the post - lunch dip by administering a novel test of mental function , changes in mental ability were assessed in a covert manner on a repetitive task using a modified test of implicit memory . this test involved a wfc task where participants were shown a fragment ( e.g. _ ha_ad _ ) and were asked to fill in the missing spaces to make a word ( e.g. charade ) . for assessing implicit memory , this task usually involves prior presentation of the unfragmented words in a long list ( 24 ) . later , fragments of those words are presented along with fragments of new words , and participants are asked to complete the fragments ( 24 ) . the increased likelihood of participants completing the fragments with previously studied words is considered priming ( 25 ) . in the present study , due to its monotonous nature , this task was not only unlikely to arouse participants out of the post - lunch dip , but may have augmented its development . fragments were considered incomplete if there was no response , if the response was spelled incorrectly or if the response was not a primed word . six of the fragments in the first task were repeated in the final task to serve as the test of implicit memory , and only the proportion of these words completed correctly is reported . thirty - six words were used as priming words , divided into six sets of six words that were counter - balanced among the thirty - six participants and three sessions in order to avoid confounding by word set . as there were not enough fragments in the source used ( 26 ) , some additional distraction fragments were introduced by the researchers . an affective post - lunch dip state was expected to be induced and maintained after 30 minutes of performing the wfc tasks based on a previous study attempting to induce the post - lunch dip through a letter cancellation task ( 27 ) . the final fragment completion task was performed by participants approximately 4767 minutes after finishing lunch , so they were within the expected post - lunch dip time both by the previous study and by indications that the post - lunch dip occurs one hour after beginning lunch ( 28 ) . further , because debate exists regarding whether the post - lunch dip is related to consuming lunch or circadian rhythms , the study was designed to administer the final fragment completion task between approximately 13:30 and 14:45 hours , depending on when participants arrived and how quickly they consumed lunch . thus , the timing of this study was designed so that participants completed the fourth fragment completion task both at the appropriate time of day and period of time after consuming lunch for the post - lunch dip to occur . further , seeking effects between 45 and 65 minutes after consuming the juice coincides with other research documenting acute effects of grape polyphenol consumption . these acute effects include improved flow mediated dilatation from dealcoholized red wine as compared to regular red wine in coronary artery disease patients ( 29 ) and increased serum total antioxidant capacity ( 30 ) . finally , as several participants requested , but were denied , a cigarette smoking break upon completion of lunch , participants may have experienced further deficits due to forced smoking abstinence during this timeframe . thus , participants were tested with the final wfc task just as the gj polyphenols were expected to begin inducing physiological effects , and the post - lunch dip , smoking abstinence and tedium of a repetitive task were likely to impair their ability / motivation . participants were compensated financially for participation , and the study protocol was approved by the purdue university institutional review board using signed informed consent . upper and lower outliers were removed if they were greater or lower than the value of the third or first quartile plus one and one - half times the interquartile range , respectively ( 31 ) . the proportion correct on wfc tasks ( by - person and by - word ) and the post - lunch dip affective state were analyzed for a main effect of time and a time - by - juice interaction using repeated measures analysis of variance . food intake and appetite ratings were analyzed for differences between treatments using the wilcoxon signed ranks test . upper and lower outliers were removed if they were greater or lower than the value of the third or first quartile plus one and one - half times the interquartile range , respectively ( 31 ) . the proportion correct on wfc tasks ( by - person and by - word ) and the post - lunch dip affective state were analyzed for a main effect of time and a time - by - juice interaction using repeated measures analysis of variance . food intake and appetite ratings were analyzed for differences between treatments using the wilcoxon signed ranks test . thirty - three participants provided saliva samples , and one individual had a cotinine concentration below the 713 ng / ml cutoff recommended for identifying smokers ( 32 ) so was not included in further analyses . the  by - person  and  by - word  analyses , the proportion correct for word fragments before lunch was unexpectedly and significantly higher in the gj treatment : f(1 , 27 )  =  7.01 , p=0.01 and f(1 , 35 )  =  4.84 , p=0.04 , respectively ( table 1 ) . there was no time - by - juice interaction or main effect of time . mean ( standard deviation ) for repeated word fragment completion ( wfc ) , hunger , fullness and energy intake * significantly higher than placebo ( p<0.05 ) . for the ag ( table 2 ) , there were no time - by - juice interactions , but there was a significant overall effect of time for both pleasure - displeasure , f(3.6 , 115.0 )  =  2.89 , p=0.03 , and arousal - sleepiness , f(3.0 , 97.9 )  =  12.52 , p<0.01 . for pleasure - displeasure , the final two post - lunch measurements were significantly lower than baseline . for arousal - sleepiness , affect grid ratings , mean ( standard error ) time effects * significantly different from baseline measurement ( p<0.05 ) . for the poms questionnaire ( table 3 ) , there were no time - by - juice interactions . however , time had an overall significant effect for confusion - bewilderment , f(1.9 , 59.0 )  =  4.14 , p=0.02 ; fatigue - inertia , f(1.4 , 41.2 )  =  15.37 , p<0.01 ; total mood disturbance ( tmd ) , f(1.7 , 50.6 )  =  10.03 , p<0.01 ; and vigor - activity , f(2.0 , 66.0 )  =  34.78 , p<0.01 . for confusion - bewilderment , fatigue - inertia and tmd , the second rating was not different from baseline , but the third rating was significantly higher than baseline . for vigor - activity , profile of mood state ( poms ) , ratings mean ( standard error ) time effects * significantly different from baseline measurement ( p<0.05 ) . for the appetite ratings , during the free - living period after lunch , at least 50% of participants provided entries hourly from 15:00 to 21:00 hours . the data from those seven time points , along with the two in - laboratory entries provided after lunch , were used to determine mean , peak and nadir ratings . there were no significant differences in hunger , fullness or energy intake ( table 1 ) . for the cognitive component , this study mirrored a recommendation from research on cocoa beverages to test participants in a state of fatigue . this augments identification of treatment effects ( 12 ) as it allows for measurement of revival of function or protection from a performance decline ( 1 ) . this study did so through use of the post - lunch dip , which is the deterioration in mental function that often occurs after consuming the mid - day meal ( 33 ) . all measures of mood were significantly modified over time in the direction expected to indicate the presence of a post - lunch dip by the time of the final mood assessment . however , the lack of time - by - juice interactions indicates beverage type had no effect on subjective feelings of the post - lunch dip . to further enhance measurement sensitivity , participants with likely increased risk for oxidative stress this was accomplished by recruiting individuals who smoked at least three cigarettes daily for at least one year ( 34 ) . this sub - group may be more likely to benefit from antioxidant treatment and have been used previously in research on grape products as a model of oxidative stress ( 35 ) . smokers also tend to have low antioxidant vitamin intake ( 36 ) . as wfc performance was not significantly changed over time for either treatment , it appears gj does not affect implicit memory and/or that performance on individual words is too stable to be affected . also , wfc rates before lunch were consistently higher in the gj treatment , which may have predisposed the results toward non - significance or favoring the placebo as there was less freedom to improve over time in the gj treatment . this study failed to detect effects of acute gj consumption by smokers on measures of appetite / food intake , mood and implicit memory . future research should explore the effects of a chronic dosing regimen on a wider variety of cognitive tasks , physiological measures and possibly a larger and different subset of participants ( e.g. nonsmokers ) . 5 p50 at00477 from the national center for complimentary and alternative medicine ( ncaam ) and the office of dietary supplements and a hatch grant from the usda # ind 084055h .
backgroundanimal experiments document effects of grape juice on cognitive performance and motor skills , and observational studies in humans suggest an inverse association between flavonoid intake and cognitive decline . 
 these effects may be related to the antioxidant properties of polyphenols . 
 juice consumption and flavonoid intake may also affect appetite.objectiveto study the acute effects of grape juice consumption on appetite , mood and implicit memory during a time of increased lethargy 
 the post - lunch dip.designthirty-five participants with a mean age of 26 years who smoked a mean of 11 cigarettes / day for 8 years were included in the study . 
 it included a practice session and two treatment sessions . 
 all sessions involved consumption of grape juice or an energy - matched placebo with lunch followed by assessments of mood , implicit memory , appetite and food intake.resultsmood decreased over time for both treatments , but there were no differences after lunch between grape juice and placebo for any measure.conclusionthis study did not document any acute effects of grape juice consumption on mood , implicit memory , appetite or food intake in smokers .