vahlkampfiids , acanthamoebidae and techamoebidae are among the important free - living amoebae ( fla ) and they could lead to severe disease including amoebic keratitis ( ak ) , granulomatose encephalitis and cutaneous ulcers ( 13 ) . free - living amoebae belonging to acanthamoeba genus are distributed in many environmental sources such as soil , clay , dust , fresh waters , mineral springs , sea , water - air interface , spas , jacuzzis and hot springs ( 4 , 5 ) . however , despite the widespread distribution of acanthamoeba in the environmental sources the incidence of disease related to these genera is not high . thus , it is reasonable to predict that not all people are susceptible to such infections ( 6 , 7 ) . indeed , the high - risk people can be divided in two categories : contact lens wearers and immunosuppressed patients including hiv positive patients , graft patients , patients undergoing corticosteroid and chemotherapy , pregnant women , diabetes , cirrhosis and lupus patients . corneal trauma even in microscopic form can lead to acanthamoeba keratitis ( 8 , 9 ) . the mannose level of cornea in healing epithelium is twofold more than normal cornea and thus the capacity of acanthamoeba binding is much higher in this situation ( 1012 ) . acanthamoeba trophozoites are flat in shape with large karyo - some and multiple food vacuoles . , various shape of endocyst may be seen in culture including triangular , square , and round forms . to date , acanthamoeba has been divided into 18 different genotypes ( t1t18 ) being most of them the cause of human infections such as severe keratitis ( 12 , 13 ) . the genetic typing is based on 18s rrna gene and the sequencing of diagnostic fragment 3 ( df3 ) of 18s rrna gene ( 14 ) . previously only t4 , t3 and t2 were introduced as a causal agent of acanthamoeba related infections ; however , later it was shown that many genotypes could lead to acanthamoeba keratitis ( ak ) including t11 , t13 , t15 , etc . unfortunately , most infections because of acanthamoeba show poor prognosis and the treatment using topical propamidin isethionate ( 0.1% ) and neosporin is still challenging ( 1 , 2 , 13 ) . diagnosis of ak is mainly based on cultivation of corneal scrapes , contact lenses on non - nutrient agar along with heat killed eschershia coli , and molecular based analysis . confocal microscopy could be non - invasive diagnosis approaches , which could be helpful for ak diagnosis and monitoring , however by using this microscopy - based test , it is not possible to differentiate between various free - living amoebae and therefore culture of corneal specimen could be the gold standard ( 15 ) . overall , the present research aimed to review the occurrence of acanthamoeba spp . in addition , their genotypes based on 18s rrna gene in environmental samples and clinical sources such as corneal scrape contact lenses and their paraphernalia . in addition , the present review highlights the increasing trend of amoebic keratitis in the country , which needs improved education regarding this opportunistic free - living protozoan . we conducted a systematic review based on the database sources such as medline , pubmed , scopus and google scholar . we searched all valuable and relevant information considering the occurrence of the acanthamoeba in both environmental and clinical samples . we referred to the information databases of medline , pubmed , scopus and google scholar and the used keywords were the combinations of acanthamoeba and amoebic keratitis , and words associated with environmental sources such as ; acanthamoeba and soil , acanthamoeba and water , acanthamoeba and dust sources , acanthamoeba plus treatment . we also referred to some information in books associated with issue of acanthamoeba and health hazard abstracts and full articles that were written in english and relevant to the topic were enrolled in this study and critically studied in detail . there are various researches , which has conducted in iran as following . the first research regarding environmental acanthamoeba distribution these investigators showed the presence of acanthamoeba and naegleria in water samples of kazeroon city using morphological key ( 15 ) . however , at that time , there was no report regarding various genotypes of acanthamoeba genus and these amoebas classified using morphological based criteria . later , another morphological - based research was done by rezaeian et al . interestingly , all of the soil samples ( 5 out of 5 ) were positive in the culture . in addition , out of 61 dust samples , 28 of them contained acanthamoeba ( 16 ) . consequently , niyyati et al . performed a sequencing based test for the mentioned environmental sources and the genotypes were belonged to t2 , t6 , t4 and t11 ( 17 ) . it is important to mention that all of isolated genotypes have been reported as a cause of ak . revealed that acanthamoeba present in pond water sources of all districts of tehran , iran . genotyping of strains showed that they were belonged to t4 and t5 genotype , both genotypes has been identified as corneal pathogens mainly in soft contact lens wearers ( 18 ) . in another research , niyyati et al . showed the presence of potentially pathogenic acanthamoeba spp . in recreational river waters of tehran , briefly , 55 water samples from 10 major rivers were analyzed for fla and identified by morphological - based criteria , pcr amplification and sequencing analysis . acanthamoeba , assigned to the t4 and t15 genotype were then identified using sequencing of df3 region ( 19 ) . other researchers showed the presence of thermotolerant acanthamoeba in hot spring of northwester iran ( 2022 ) . conducted a survey regarding the pathogenic potential of acanthamoeba t4 type using osmo - tolerance and thermo - tolerance assay . cysts isolated from water , soil and dust ( star shape cysts are shown in clump ) ( magnification : a : x10 ; b , c , d : x40 ) ( figures are from pathogenic free living amoebae in human book , rezaeian m and niyyati m , 2009 ) acanthamoeba spp . trophozoites isolated from soil and dust ( flat shape trophozoites are shown ) ( magnification : a : x10 , b : x40 ) rezaeian et al . was the first to report the presence of acanthamoeba in hospital dusts in tehran , iran . this finding could be a health hazard for high - risk people including contact lens wearers and patients with eye surgery ( 16 ) . the occurrence of fla in immunodeficiency wards of hospitals in tehran , iran were also investigated by lasjerdi et al . briefly , 70 dust and biofilm samples from immunodeficiency wards of university hospitals were collected and tested for the presence of fla using culturing and molecular approaches presence of the t4 genotype on medical instruments , including an oxygen mask in an isolation room of an immunodeficiency ward , should be of concern for health authorities . overall , these results reflect a clear need for improved disinfection , especially where high - risk people , such as those who are immune - suppressed or undergo eye surgery such as lasic surgeries , are served . encephalitis due to acanthamoeba has not been reported yet , mainly due to lack of knowledge regarding acanthamoeba as an agent of central nervous system infections . so far , there are 150 cases of ak in iran , although it seems that these numbers is lower than the true cases . the first cases of ak in iran were reported in a soft contact lens wearer ( 15 ) . in 2007 , interestingly , 44 patients ( 89.79% ) were contact lens wearers for cosmetic purposes or visual corrections . among them 41 patients ( 93.18% ) wore soft contact lenses and three patients were hard contact lens wearers ( 25 ) . among 50 keratitis patient , 13 were positive for acanthamoeba ( 26 ) . three species including a. griffin , a. palesinensis and a. castellanii were identified in their samples . another study revealed acanthamoeba as a causal agent in 15 ( 30% ) of 50 keratitis samples . among these clinical isolates , 13 ( 86.7% ) belonged to female patients and 2 ( 13.3% ) were male . all positive specimens belonged to soft contact lens wearers and only one belonged to a patient with a history of hard contact lens usage . regarding genotype identification , 13 ( 86.7% ) of these isolates belonged to t4 genotype . however , it is important to mention there was a mixed genotype belonging to acanthamoebae t4 and t11 genotypes in one patient . other genotypes identified in the clinical specimens were t11 ( 13.3% ) and t3 ( 6.7% ) ( 17 ) . another survey of the 90 asymptomatic contact lens wearers , 9 ( 10% ) were positive for fla outgrowth . morphological analysis revealed that 3 isolates were belonged to hartmannella genus according to small round cysts and 6 isolates were belonged to acanthamoeba genus based on the star shape of endocysts . sequencing revealed that acanthamoeba belonged to t4 , t3 and t5 genotype ( 27 ) . cysts isolated from acanthamoeba keratitis ( triangular shape cysts are shown ) ( magnification : x10 ) acanthamoeba cysts isolated from acanthamoeba keratitis ( triangular shape cysts are shown ) ( magnification : right : x10 , left : x40 ) so far , other infections due to acanthamoeba spp . have not been reported in iran . this is due to lack of knowledge regarding these opportunistic protozoan parasites in this region . it should be noted that memari et al . reported that acanthamoeba belonging to potentially pathogenic t3 , t4 and t5 genotypes could be colonize in nasal mucosa of cancer patients which this could be a serious health hazard for developing gae ( 28 ) . regarding treatment approaches khojaste et al . conducted a study targeting the gene profile of acanthamoeba t4 type in trophozoites and cysts . the result revealed that three genes including heat shock protein 70 ( hsp70 ) , actin - i and elongation factor-1alpha ( ef-1 ) were differentially expressed during acanthamoeba differentiation and they could be the novel target for treatment ( 29 ) . a recent research by niyyati et al . also reported that tap and filtrated waters could be the sources of pathogenic free - living amoebae and this could render contact lens wearers for developing ak ( 30 ) . there are various researches , which has conducted in iran as following . the first research regarding environmental acanthamoeba distribution these investigators showed the presence of acanthamoeba and naegleria in water samples of kazeroon city using morphological key ( 15 ) . however , at that time , there was no report regarding various genotypes of acanthamoeba genus and these amoebas classified using morphological based criteria . interestingly , all of the soil samples ( 5 out of 5 ) were positive in the culture . in addition , out of 61 dust samples , 28 of them contained acanthamoeba ( 16 ) . consequently , niyyati et al . performed a sequencing based test for the mentioned environmental sources and the genotypes were belonged to t2 , t6 , t4 and t11 ( 17 ) . it is important to mention that all of isolated genotypes have been reported as a cause of ak . revealed that acanthamoeba present in pond water sources of all districts of tehran , iran . genotyping of strains showed that they were belonged to t4 and t5 genotype , both genotypes has been identified as corneal pathogens mainly in soft contact lens wearers ( 18 ) . in another research , niyyati et al . showed the presence of potentially pathogenic acanthamoeba spp . in recreational river waters of tehran , briefly , 55 water samples from 10 major rivers were analyzed for fla and identified by morphological - based criteria , pcr amplification and sequencing analysis . acanthamoeba , assigned to the t4 and t15 genotype were then identified using sequencing of df3 region ( 19 ) . other researchers showed the presence of thermotolerant acanthamoeba in hot spring of northwester iran ( 2022 ) . conducted a survey regarding the pathogenic potential of acanthamoeba t4 type using osmo - tolerance and thermo - tolerance assay . cysts isolated from water , soil and dust ( star shape cysts are shown in clump ) ( magnification : a : x10 ; b , c , d : x40 ) ( figures are from pathogenic free living amoebae in human book , rezaeian m and niyyati m , 2009 ) acanthamoeba spp . trophozoites isolated from soil and dust ( flat shape trophozoites are shown ) ( magnification : a : x10 , b : x40 ) rezaeian et al . was the first to report the presence of acanthamoeba in hospital dusts in tehran , iran . this finding could be a health hazard for high - risk people including contact lens wearers and patients with eye surgery ( 16 ) . the occurrence of fla in immunodeficiency wards of hospitals in tehran , iran were also investigated by lasjerdi et al . briefly , 70 dust and biofilm samples from immunodeficiency wards of university hospitals were collected and tested for the presence of fla using culturing and molecular approaches . presence of the t4 genotype on medical instruments , including an oxygen mask in an isolation room of an immunodeficiency ward , should be of concern for health authorities . overall , these results reflect a clear need for improved disinfection , especially where high - risk people , such as those who are immune - suppressed or undergo eye surgery such as lasic surgeries , are served . . encephalitis due to acanthamoeba has not been reported yet , mainly due to lack of knowledge regarding acanthamoeba as an agent of central nervous system infections . so far , there are 150 cases of ak in iran , although it seems that these numbers is lower than the true cases . the first cases of ak in iran were reported in a soft contact lens wearer ( 15 ) . in 2007 , interestingly , 44 patients ( 89.79% ) were contact lens wearers for cosmetic purposes or visual corrections . among them 41 patients ( 93.18% ) wore soft contact lenses and three patients were hard contact lens wearers ( 25 ) . among 50 keratitis patient , 13 were positive for acanthamoeba ( 26 ) . three species including a. griffin , a. palesinensis and a. castellanii were identified in their samples . another study revealed acanthamoeba as a causal agent in 15 ( 30% ) of 50 keratitis samples . among these clinical isolates , 13 ( 86.7% ) belonged to female patients and 2 ( 13.3% ) were male . all positive specimens belonged to soft contact lens wearers and only one belonged to a patient with a history of hard contact lens usage . regarding genotype identification , 13 ( 86.7% ) of these isolates belonged to t4 genotype . however , it is important to mention there was a mixed genotype belonging to acanthamoebae t4 and t11 genotypes in one patient . other genotypes identified in the clinical specimens were t11 ( 13.3% ) and t3 ( 6.7% ) ( 17 ) . another survey of the 90 asymptomatic contact lens wearers , 9 ( 10% ) were positive for fla outgrowth . morphological analysis revealed that 3 isolates were belonged to hartmannella genus according to small round cysts and 6 isolates were belonged to acanthamoeba genus based on the star shape of endocysts . sequencing revealed that acanthamoeba belonged to t4 , t3 and t5 genotype ( 27 ) . cysts isolated from acanthamoeba keratitis ( triangular shape cysts are shown ) ( magnification : x10 ) acanthamoeba cysts isolated from acanthamoeba keratitis ( triangular shape cysts are shown ) ( magnification : right : x10 , left : x40 ) so far , other infections due to acanthamoeba spp . have not been reported in iran . this is due to lack of knowledge regarding these opportunistic protozoan parasites in this region . it should be noted that memari et al . reported that acanthamoeba belonging to potentially pathogenic t3 , t4 and t5 genotypes could be colonize in nasal mucosa of cancer patients which this could be a serious health hazard for developing gae ( 28 ) . regarding treatment approaches khojaste et al . conducted a study targeting the gene profile of acanthamoeba t4 type in trophozoites and cysts . the result revealed that three genes including heat shock protein 70 ( hsp70 ) , actin - i and elongation factor-1alpha ( ef-1 ) were differentially expressed during acanthamoeba differentiation and they could be the novel target for treatment ( 29 ) . a recent research by niyyati et al . also reported that tap and filtrated waters could be the sources of pathogenic free - living amoebae and this could render contact lens wearers for developing ak ( 30 ) . the ubiquity of acanthamoeba spp . in environmental sources such as fresh waters , hot springs and tap waters and recreational soil sources and also the occurrence of the amoebae in the dust and biofilm samples of hospitals and clinical settings in iran and around the world could be a health concern specially for those who are within high risk groups such as contact lens wearers and immunosuppressed patients . on the other hand , ak continues to rise in iran mainly in female soft contact lens wearers with a history of poor maintenance of their lenses . there are no reports of acanthamoeba encephalitis in the region yet and thus this point reflects the need for more researches in suspected encephalitis patients . here , according to our thorough review acanthamoeba belonging to t4 genotype is the most prevalent type strain in environmental and clinical samples in several regions in iran and worldwide , however , there are reports regarding acanthamoeba belonging to other genotypes such as t2 and the mentioned point could leads us to more researches with the goal of presenting the real genotype dominance of acanthamoeba and related disease in this country . additionally researches regarding new treatment strategies for acanthamoeba - related infections are an utmost priority topic as such infections are manifest with poor prognosis .
background : free - living amoebae belonging to the genus acanthamoeba have an environmental distribution . 
 amoebic keratitis due to these protozoan parasites continue to rise in iran and worldwide . in iran , there are various researches regarding both morphological and molecular identification of acanthamoeba spp . in environmental and clinical samples . 
 however , there is no thorough review about acanthamoeba genotypes and their distribution in environmental sources such as water , dust and biofilm in iran . 
 besides , according to increasing cases of amoebic keratitis in the region awareness regarding the pathogenic potential of these sight - threatening amoebae is of utmost importance.methods:we conducted a thorough review based on the database sources such as medline , pubmed and google scholar . 
 no restrictions were placed on study date , study design or language of publication . 
 we searched all valuable and relevant information considering the occurrence of the acanthamoeba in both environmental and clinical samples.results:according to our thorough review acanthamoeba belonging to t4 genotype is the most prevalent type strain in environmental and clinical samples in several regions in iran and worldwide , however , there are reports regarding acanthamoeba belonging to other genotypes such as t2 , t3 , t5 , t6 and t11 and the mentioned point could leads us to more researches with the goal of presenting the real genotype dominance of acanthamoeba and related disease in the country.conclusion:overall , the present review will focus on present status of genotypes of acanthamoeba in iran during recent years .