typical tunisian dietary foods were chosen with reference to a number of epidemiologic studies ( 2224 ) . the aim was to include a wide range of foods that require some aid to assess portion sizes in a questionnaire and to span the selected visual appearance which is likely to influence perception of amounts from photos . construction of the food photography manual involved cooking , weighing , and taking digital photographs of three different portion sizes : small ( a ) , medium ( b ) , and large portion ( c ) . foods that needed to be cooked ( vegetables , pasta , meat , fish , etc . ) were weighed before and after cooking . two types of scales of different capacity were used to weigh the different portions ( a precision scale chimazou , bw 420 h with a precision of 0.001 g and a maximum capacity of 420 g , and a daewoo dks 2055 scale with a precision of 1 g and a maximum capacity of 5,000 g ) . digital photographs of three identified portions were taken under standard lighting conditions in a room by a professional photographer using a digital camera ( canon ( r ) 40 d lens , canon 70200 mm ) . the three portion sizes considered to be representative of the range of portion sizes actually consumed ( 2224 ) were placed on a white dining plate ( 26 cm in diameter ) in a colored background contrast . all photographs were taken at approximately the same angle of 45 and from a distance of 50 cm above the plate , which was considered the best compromise between showing the depth and height of the food ( 25 ) . this technique , sitting at a table , is necessary to show the differences between the size of the portions depending on the height of the food on a plate and depth in a soup bowl ( 15 ) . this offers the best compromise by showing the size and the depth of the portion , the number and size of items , size and slice thickness , depth in a bowl , etc . these appearance characteristics are likely to influence the perception of portion sizes ( 2628 ) . the 400 photos in the manual cover eight food groups : bread , cereals ( including pasta , rice , couscous ) ; potatoes , vegetables ( raw and cooked ) ; fresh fruits , animal products ( meat and meat products , fish and fish products , eggs ) ; dairy products ( milk , cheese ) ; and sweet products . each page of the manual presents three sets of three pictures of three different foods . each food was presented in three portion sizes : small , medium and large ( labelled a , b , and c , respectively ) that are considered representative of the tunisian population consumption . but an incorporated scale enables the person to select intermediate sizes as well , giving a total of seven portion sizes : < a , a , a  b , b , b c , c , and > c. the actual weight of the portions of raw or cooked food was presented in the appendix of the manual . this manual has the advantage of being the first to represent a wide list of foods usually consumed by the tunisian population , for which a visual aid to estimate portion sizes is necessary ( 6 , 7 ) . the photographs were designed to help estimate the amount of foods consumed the previous day by the 24-hour recall method . example of photographs from the photos manual shows portion sizes of food and the amounts in grams . ( a ) couscous , ( b ) fish , ( c ) fresh vegetables , ( d ) apples . thirty - one volunteers of both sexes in the cap - bon governorate ( bouargoub town ) were invited to participate in the study . a nutritionist from the same region visited the participants in their homes and explained the procedure of the study and its purpose , explaining that the aim was to assess the usual dietary intake of the participants by the fw method but without indicating that the portion sizes of the foods consumed would also be evaluated on the next day . their participation depended on their willingness to be followed closely for 1 day during the preparation and consumption of their meals . on the first day , participants received the investigator at home during the preparation and consumption of three main meals . the dietary survey technique used to estimate nutrient intake was fw , which was considered as the reference method ( 3 ) . for each meal , the amount of foods consumed by each individual was recorded and weighed by the trained dietician at the time of serving . if any food was left over , the weight was recorded , and the amount of food eaten was calculated as the difference between the amount served and the amount left . in the case of mixed meals , the raw ingredients used in their preparation were weighed ( 0.1 g ) . the final total weight of the mixed dish was weighed using a second scale with greater capacity ( 5,0001 g ) . the body mass index ( bmi ) was computed as weight ( kg)/height squared ( m ) and evaluated using the world health organization classification ( 29 ) . on the following day , the investigator conducted a modified 24-hour recall ( fp 24-hr ) to estimate the amount of food consumed by the participants the day before . the participants were asked to recall the food intake during the preceding day . for each food , they were asked to mark a cross on the manual photos that they believed represented most closely the size of the portion they had consumed . the dietary intakes of participants estimated by the fp 24-hr using the photographic manual and the fw method were transformed into key macro and micronutrients using the bilnut software . to validate the fp 24-hr method , we used three tests : the differences between amounts in portion sizes of food categories weighed and estimated were tested using wilcoxon signed rank test ( data not normally distributed ) , and differences between nutrient intake estimated by fp 24-hr and fw were tested using paired t-test.pearson's or spearman 's rank correlation coefficients were calculated to assess the association between the weighed and estimated amounts of food ( spearman ) and also nutrient intake ( pearson).agreement between the two methods was evaluated according to bland the mean differences of food amounts and nutrients intake between the two methods were plotted against their average value , and the 95% limits of agreement and the 95% confidence interval ( ci ) were marked . the differences between amounts in portion sizes of food categories weighed and estimated were tested using wilcoxon signed rank test ( data not normally distributed ) , and differences between nutrient intake estimated by fp 24-hr and fw were tested using paired t - test . pearson 's or spearman 's rank correlation coefficients were calculated to assess the association between the weighed and estimated amounts of food ( spearman ) and also nutrient intake ( pearson ) . agreement between the two methods was evaluated according to bland  altman analysis ( 30 ) . the mean differences of food amounts and nutrients intake between the two methods were plotted against their average value , and the 95% limits of agreement and the 95% confidence interval ( ci ) were marked . statistical analysis was done by spss version 20.0 software . the significance level for statistical tests typical tunisian dietary foods were chosen with reference to a number of epidemiologic studies ( 2224 ) . the aim was to include a wide range of foods that require some aid to assess portion sizes in a questionnaire and to span the selected visual appearance which is likely to influence perception of amounts from photos . construction of the food photography manual involved cooking , weighing , and taking digital photographs of three different portion sizes : small ( a ) , medium ( b ) , and large portion ( c ) . foods that needed to be cooked ( vegetables , pasta , meat , fish , etc . ) were weighed before and after cooking . two types of scales of different capacity were used to weigh the different portions ( a precision scale chimazou , bw 420 h with a precision of 0.001 g and a maximum capacity of 420 g , and a daewoo dks 2055 scale with a precision of 1 g and a maximum capacity of 5,000 g ) . digital photographs of three identified portions were taken under standard lighting conditions in a room by a professional photographer using a digital camera ( canon ( r ) 40 d lens , canon 70200 mm ) . the three portion sizes considered to be representative of the range of portion sizes actually consumed ( 2224 ) were placed on a white dining plate ( 26 cm in diameter ) in a colored background contrast . all photographs were taken at approximately the same angle of 45 and from a distance of 50 cm above the plate , which was considered the best compromise between showing the depth and height of the food ( 25 ) . this technique , sitting at a table , is necessary to show the differences between the size of the portions depending on the height of the food on a plate and depth in a soup bowl ( 15 ) . this offers the best compromise by showing the size and the depth of the portion , the number and size of items , size and slice thickness , depth in a bowl , etc . these appearance characteristics are likely to influence the perception of portion sizes ( 2628 ) . the 400 photos in the manual cover eight food groups : bread , cereals ( including pasta , rice , couscous ) ; potatoes , vegetables ( raw and cooked ) ; fresh fruits , animal products ( meat and meat products , fish and fish products , eggs ) ; dairy products ( milk , cheese ) ; and sweet products . each page of the manual presents three sets of three pictures of three different foods . each food was presented in three portion sizes : small , medium and large ( labelled a , b , and c , respectively ) that are considered representative of the tunisian population consumption . but an incorporated scale enables the person to select intermediate sizes as well , giving a total of seven portion sizes : < a , a , a  b , b , b c , c , and > c. the actual weight of the portions of raw or cooked food was presented in the appendix of the manual . this manual has the advantage of being the first to represent a wide list of foods usually consumed by the tunisian population , for which a visual aid to estimate portion sizes is necessary ( 6 , 7 ) . the photographs were designed to help estimate the amount of foods consumed the previous day by the 24-hour recall method . example of photographs from the photos manual shows portion sizes of food and the amounts in grams . ( a ) couscous , ( b ) fish , ( c ) fresh vegetables , ( d ) apples . thirty - one volunteers of both sexes in the cap - bon governorate ( bouargoub town ) were invited to participate in the study . a nutritionist from the same region visited the participants in their homes and explained the procedure of the study and its purpose , explaining that the aim was to assess the usual dietary intake of the participants by the fw method but without indicating that the portion sizes of the foods consumed would also be evaluated on the next day . their participation depended on their willingness to be followed closely for 1 day during the preparation and consumption of their meals . , participants received the investigator at home during the preparation and consumption of three main meals . the dietary survey technique used to estimate nutrient intake was fw , which was considered as the reference method ( 3 ) . for each meal , the amount of foods consumed by each individual was recorded and weighed by the trained dietician at the time of serving . if any food was left over , the weight was recorded , and the amount of food eaten was calculated as the difference between the amount served and the amount left . in the case of mixed meals , the raw ingredients used in their preparation were weighed ( 0.1 g ) . the final total weight of the mixed dish was weighed using a second scale with greater capacity ( 5,0001 g ) . besides , the investigator measured the height and weight of the participants . the body mass index ( bmi ) was computed as weight ( kg)/height squared ( m ) and evaluated using the world health organization classification ( 29 ) . on the following day , the investigator conducted a modified 24-hour recall ( fp 24-hr ) to estimate the amount of food consumed by the participants the day before . the participants were asked to recall the food intake during the preceding day . for each food , they were asked to mark a cross on the manual photos that they believed represented most closely the size of the portion they had consumed . the dietary intakes of participants estimated by the fp 24-hr using the photographic manual and the fw method were transformed into key macro and micronutrients using the bilnut software . to validate the fp 24-hr method , we used three tests : the differences between amounts in portion sizes of food categories weighed and estimated were tested using wilcoxon signed rank test ( data not normally distributed ) , and differences between nutrient intake estimated by fp 24-hr and fw were tested using paired t-test.pearson's or spearman 's rank correlation coefficients were calculated to assess the association between the weighed and estimated amounts of food ( spearman ) and also nutrient intake ( pearson).agreement between the two methods was evaluated according to bland the mean differences of food amounts and nutrients intake between the two methods were plotted against their average value , and the 95% limits of agreement and the 95% confidence interval ( ci ) were marked . the differences between amounts in portion sizes of food categories weighed and estimated were tested using wilcoxon signed rank test ( data not normally distributed ) , and differences between nutrient intake estimated by fp 24-hr and fw were tested using paired t - test . pearson 's or spearman 's rank correlation coefficients were calculated to assess the association between the weighed and estimated amounts of food ( spearman ) and also nutrient intake ( pearson ) . the mean differences of food amounts and nutrients intake between the two methods were plotted against their average value , and the 95% limits of agreement and the 95% confidence interval ( ci ) were marked . statistical analysis was done by spss version 20.0 software . the significance level for statistical tests participants were aged between 9 and 89 ( mean : 33.614.5 years ) . of the 31 participants , the bmi of children and adolescents was 24.12.2 kg / m . according to cole 's cutoff point reference values ( 31 ) , 43.8 and 14.2% of children were overweight and obese , respectively . the bmi of adults was 25.74.9 kg / m ( 24.73.1 and 26.35.8 , for males and females , respectively ) . nevertheless , 11.1% of males and 13.3% of females were overweight ( 25  bmi < 30 ) and 11.1% of males and 40% of females are obese ( bmi  30 ) . for the whole sample , prevalence of overweight and obesity was 12.5 and 29.1% , respectively . the overall assessments were grouped in the following eight major food categories : animal products ( meat and meat products , fish and fish products , and eggs ) ; vegetables ( raw and cooked ) , potatoes ; fruits ( fresh ) ; bread , pasta , dairy products ( milk and dairy products ) ; and sweet products . the amounts of food ( mean and standard deviation ) estimated by fp 24-hr and actually consumed ( fw ) and the differences in the means are presented in table 1 . all food categories were overestimated ( ranged from 2.2% for dairy products to 18.6% for pasta ) , but animals products and sweet products were underestimated by 9.9 and 4.5% , respectively . statistical analysis by paired t - test series shows that the differences between the amounts estimated by fp 24-hr and those weighed were not statistically significant , except for pasta ( p<0.01 ) and dairy products ( p<0.05 ) . the correlation coefficient was calculated to study the relationship at the individual level between the estimated and the actually consumed amounts . a highly significant correlation ( p<0.001 ) the value of r ranged from 0.882 ( potatoes ) to 0.989 ( dairy products ) . the agreement between the estimated and the weighed amounts for the eight food groups was assessed by bland altman plots for mean daily intakes for three of the major food groups ( meat , vegetables , and fruits ) are presented in fig . overall , these plots indicate acceptable agreement between the two methods for assessment of dairy products , potatoes , and sweet products . ( a ) meat , ( b ) vegetables , ( c ) fruits . amount of food estimated by fp 24-hr and compared with amount weighed in fw sd : standard deviation . percent of the mean difference=[(amount from fp 24-hramount from fw)/(mean amount from fw)*100 ] . the means of the estimated and the actually consumed amounts of energy , protein , fat , carbohydrates , dietary fiber , vitamin b1 , vitamin c , vitamin e , folate , calcium , iron , and zinc are presented in table 2 . the average differences between the recall and weighing methods were negative for lipids ( 8.2% ) , zinc ( 11.1% ) , vitamin b1 ( 2.4% ) , and vitamin e ( 11.6% ) . the differences were positive for protein ( 0.43% ) , carbohydrate ( 6.5% ) , fiber ( 9% ) , and vitamin c ( 3.6% ) . the differences vary insignificantly from 11.6% for vitamin e intake to 9% for fiber intake . significant differences were observed for lipid ( p<0.01 ) and fiber intake ( p<0.05 ) . correlation coefficients for the estimates of nutrient intakes between the two methods are presented in table 2 . the value of the correlation coefficient ranged from 0.644 ( folate ) to 0.985 ( vitamin b1 ) . in order to assess the agreement between the methods for all nutrients , bland altman plot for carbohydrate indicated broad limits of agreement . in addition , the difference in the estimates of carbohydrate intakes between fp 24-hr and fw became progressively greater with higher mean intakes ( fig . 3 , like carbohydrate intake , the difference in estimates of total protein ( g ) and fiber ( g ) intakes between the two methods became progressively greater with higher mean intakes . correlations varied from 0.889 ( protein ) to 0.943 ( carbohydrate ) . for micronutrients , correlation coefficients were low for folate ( 0.644 ) and high for vitamin b1 ( 0.985 ) . the majority of the measurements of calcium , iron , and vitamin e were scattered along the equality line . ( a ) protein , ( b ) fat , ( c ) carbohydrate . mean nutrient intake and comparison of the results obtained with the fp 24-hr and fw methods sd : standard deviation . participants were aged between 9 and 89 ( mean : 33.614.5 years ) . of the 31 participants , the bmi of children and adolescents was 24.12.2 kg / m . according to cole 's cutoff point reference values ( 31 ) , 43.8 and 14.2% of children were overweight and obese , respectively . the bmi of adults was 25.74.9 kg / m ( 24.73.1 and 26.35.8 , for males and females , respectively ) . nevertheless , 11.1% of males and 13.3% of females were overweight ( 25  bmi < 30 ) and 11.1% of males and 40% of females are obese ( bmi  30 ) . for the whole sample , prevalence of overweight and obesity was 12.5 and 29.1% , respectively . the overall assessments were grouped in the following eight major food categories : animal products ( meat and meat products , fish and fish products , and eggs ) ; vegetables ( raw and cooked ) , potatoes ; fruits ( fresh ) ; bread , pasta , dairy products ( milk and dairy products ) ; and sweet products . the amounts of food ( mean and standard deviation ) estimated by fp 24-hr and actually consumed ( fw ) and the differences in the means are presented in table 1 . all food categories were overestimated ( ranged from 2.2% for dairy products to 18.6% for pasta ) , but animals products and sweet products were underestimated by 9.9 and 4.5% , respectively . statistical analysis by paired t - test series shows that the differences between the amounts estimated by fp 24-hr and those weighed were not statistically significant , except for pasta ( p<0.01 ) and dairy products ( p<0.05 ) . the correlation coefficient was calculated to study the relationship at the individual level between the estimated and the actually consumed amounts . a highly significant correlation ( p<0.001 ) the value of r ranged from 0.882 ( potatoes ) to 0.989 ( dairy products ) . the agreement between the estimated and the weighed amounts for the eight food groups was assessed by bland altman plots for mean daily intakes for three of the major food groups ( meat , vegetables , and fruits ) are presented in fig . overall , these plots indicate acceptable agreement between the two methods for assessment of dairy products , potatoes , and sweet products . ( a ) meat , ( b ) vegetables , ( c ) fruits . amount of food estimated by fp 24-hr and compared with amount weighed in fw sd : standard deviation . the means of the estimated and the actually consumed amounts of energy , protein , fat , carbohydrates , dietary fiber , vitamin b1 , vitamin c , vitamin e , folate , calcium , iron , and zinc are presented in table 2 . the average differences between the recall and weighing methods were negative for lipids ( 8.2% ) , zinc ( 11.1% ) , vitamin b1 ( 2.4% ) , and vitamin e ( 11.6% ) . the differences were positive for protein ( 0.43% ) , carbohydrate ( 6.5% ) , fiber ( 9% ) , and vitamin c ( 3.6% ) . the differences vary insignificantly from 11.6% for vitamin e intake to 9% for fiber intake . significant differences were observed for lipid ( p<0.01 ) and fiber intake ( p<0.05 ) . correlation coefficients for the estimates of nutrient intakes between the two methods are presented in table 2 . the value of the correlation coefficient ranged from 0.644 ( folate ) to 0.985 ( vitamin b1 ) . in order to assess the agreement between the methods for all nutrients , bland in addition , the difference in the estimates of carbohydrate intakes between fp 24-hr and fw became progressively greater with higher mean intakes ( fig . 3 , like carbohydrate intake , the difference in estimates of total protein ( g ) and fiber ( g ) intakes between the two methods became progressively greater with higher mean intakes . correlations varied from 0.889 ( protein ) to 0.943 ( carbohydrate ) . for micronutrients , correlation coefficients were low for folate ( 0.644 ) and high for vitamin b1 ( 0.985 ) . the majority of the measurements of calcium , iron , and vitamin e were scattered along the equality line . ( a ) protein , ( b ) fat , ( c ) carbohydrate . mean nutrient intake and comparison of the results obtained with the fp 24-hr and fw methods sd : standard deviation . the aim of this work was to develop and validate a tunisian food photography manual . this manual could be used as a visual aid to improve the accuracy of the estimation of food quantities consumed by surveyed individuals . this food photography manual has the advantage of being the first to represent a wide list of foods usually consumed by the tunisian population and requiring a visual aid for estimating portion sizes . many studies have shown interest in using food photography to reduce the errors in estimating the quantities of consumed foods ( 27 , 32 , 33 ) . however , it is of utmost importance to validate the photographic manual and to determine the presence or absence of bias and its direction when estimating food amounts . comparison of the amount of consumed food estimated by the fp 24-hr method and weighing method showed that the mean differences between the two methods were not significant for all food groups , except for pasta ( 18.6% ) and dairy products ( 2.2% ) ( p<0.05 ) . some researchers ( 15 , 34 ) reported that the portion size of some foods seems to be more difficult to estimate accurately than others . de souza et al . indicated that it was easier to estimate the portions of food that are part of the food habit ( 35 ) . the consistency between portion weights and estimated portion sizes of the foods eaten is revealed by the high mean correlation coefficients . regarding nutritional intake , the average differences between the two methods ranged from 11.6 ( vitamin e ) to 8.9% ( fibers ) . these average differences in dietary intake were not statistically significant . on the contrary , the estimate of food and nutrient intakes estimated by the recall of 24 hours with photos is highly correlated with the actually consumed amounts . these results are consistent with those found in the literature ( 13 , 15 , 27 , 28 ) . in figs . 2 and 3 , the bland almost all of the points are in a band of plus or minus double the standard deviation of the straight equality of means . altman analysis shows that most measures of categories of foods and nutritional intake are scattered on either side of the mean differences and all along the line of equality ( difference=0 ) . our data analyses of individual food categories and nutrients intake show that the fp 24-hr was able to adequately estimate the weights of food portions and gave results that are comparable to the actually consumed amounts ( fw ) . the number and size of the photos used to estimate portion sizes vary from one study to another , from one to eight ( 27 ) . it has been shown that the greater the number of serving size categories , the smaller the estimation error ( 36 ) . a complex process is triggered when using photographs to identify the portion size of food during a food interrogation . these three elements depend on the number of different portion sizes , location in the manual , the size of each photo , and some characteristics of the respondents ( gender , age , body size , etc . ) however , a study in south africa ( 18 ) showed that accuracy of estimation was not affected by sex , age , or education . the estimated size of the portions was not influenced by sex and bmi of the individuals and that it was easier to estimate the portions of food that are habitual ( 35 ) . in a dietary study using the 24-hour recall method , two main tasks are required by the subject in estimating portion size : 1 ) memory of the foods eaten and 2 ) conceptualization ( i.e. the ability to translate an abstract mental impression of an amount eaten into an amount depicted in a photograph ) ( 28 ) . more recently , portion size photographs have also been developed to be used on a computer screen . recent studies have reported that the photos taken by the participants themselves when food intake occurs in many environments , for example , cafes and restaurants , can improve the accuracy of collected data ( 3840 ) . the amount of food is estimated on the basis of this comparison and the application automatically calculates the energy and nutrient intake . this use of digital photography has the advantage of reducing the burden on participants when estimating portion sizes . but it does not exclude the need for a manual of standards for evaluating the photographed portion sizes and those consumed by the subjects ( 41 , 42 ) . possible limitations of the present study are the small number of participants ( which affects the power of the statistical tests ) , wide age range , and the simplicity of the examined menus . such limits may explain the high correlations and low differences between the results of the two methods . one possible reason for the poor agreement is that respondents might be less able to conceptualize portion sizes and are less practiced in estimating measures . the validation method bears international relevance for individual - level dietary assessment , because it is one of the few studies that have used measures of agreement to test a photographic atlas for this purpose under the interest of this study is that it was conducted under the conditions of normal living on a population sample that is heterogeneous regarding sex , age , and bmi . therefore , the food consumption of the participants can be considered as representative of their usual consumption . our study may be the first using photographs to assess nutrient intake in free - living conditions in tunisia . it appears to be a useful tool for portion size estimation of the common foods in the tunisian diet . the analysis of data of food and nutrient intake shows that 24-hour recall with the use of food photos yields results that are comparable to those obtained by fw . the 24-hour recall method in combination with food photos therefore , this food photography manual can be used as a standard basis for dietary surveys to estimate the amounts of consumed food . it is a better alternative for obtaining an accurate and representative estimation of the current or usual nutrient intake in large - scale dietary surveys in tunisia . further work to develop and test sets of food photographs for use in different areas and population groups in the mediterranean region and africa is needed . mongia bouchoucha contributed to the formulation of the research , the study design , and writing the article . fadwa tarhouni and rim bouchoucha performed the food survey : 24-hour recall method and fw . mouna akrout and bchir zouari contributed to the formulation of the research and analyzed the data . this study was conducted according to the guidelines laid down in the declaration of helsinki , and all procedures involving human subjects / patients were approved by the ethic committee of abderrahman mami hospital , ariana , tunisia . verbal informed consent was obtained from all subjects : verbal consent was not formally recorded . the authors have not received any funding or benefits from industry or elsewhere to conduct this study .
backgroundestimation of food portion sizes has always been a challenge in dietary studies on free - living individuals . 
 the aim of this work was to develop and validate a food photography manual to improve the accuracy of the estimated size of consumed food portions.methodsa manual was compiled from digital photos of foods commonly consumed by the tunisian population . 
 the food was cooked and weighed before taking digital photographs of three portion sizes . 
 the manual was validated by comparing the method of 24-hour recall ( using photos ) to the reference method [ food weighing ( fw ) ] . in both the methods , 
 the comparison focused on food intake amounts as well as nutritional issues . 
 validity was assessed by bland  altman limits of agreement . in total , 
 31 male and female volunteers aged 989 participated in the study.resultswe focused on eight food categories and compared their estimated amounts ( using the 24-hour recall method ) to those actually consumed ( using fw ) . 
 animal products and sweets were underestimated , whereas pasta , bread , vegetables , fruits , and dairy products were overestimated . however , the difference between the two methods is not statistically significant except for pasta ( p<0.05 ) and dairy products ( p<0.05 ) . 
 the coefficient of correlation between the two methods is highly significant , ranging from 0.876 for pasta to 0.989 for dairy products . 
 nutrient intake calculated for both methods showed insignificant differences except for fat ( p<0.001 ) and dietary fiber ( p<0.05 ) . 
 a highly significant correlation was observed between the two methods for all micronutrients . 
 the test agreement highlights the lack of difference between the two methods.conclusionthe difference between the 24-hour recall method using digital photos and the weighing method is acceptable . 
 our findings indicate that the food photography manual can be a useful tool for quantifying food portion sizes in epidemiological dietary surveys .