multiple sequence alignments ( msas ) are an essential first step for a number of computational approaches such as protein secondary structure / function prediction , phylogeny inference , and many other common tasks in sequence analysis.15 several software tools for generating multiple sequence alignments are available,6 but none of them is suitable for all types of data sets.7 consequently , in order to generate a true alignment , a need rises for inspection and adjusting alignments by hand , which is a very laborious job.5 furthermore , handling hundreds of thousands of alignments is another problem in the domain of bioinformatics . many popular alignment editors such as jalview,5 strap,8 seaview,9 mega,10 cinema,11 and base - by - base12 are available . all these tools either do not support tens of thousands of sequences ( where length of each sequence is more than 2,500 base pairs ) or they do not have user - friendly editing features . jalview and strap do not work when the size of an alignment exceeds 30.01 mb . seaview and mega can load big alignments , but the editing features provided by them involve multiple steps . many software tools provide graphical user interfaces to reconstruct msas , but they do not allow loading multiple sequence files at the same time . msa comparison tools such as suitemsa6 and sinicview13 permit to compare msas , but they support alignments comprising less than 1,000 sequences . multiple formats of msas exist , but fasta is the most popular format . presently , there is no tool that can convert the format of an alignment of unlimited size into fasta format . many tools , such as matgat14 and sequence identity and similarity ( sias),15 are available , to calculate the identity matrix but they do not support more than 1,000 sequences . this article describes ivistmsa , which is a software package of seven graphical tools for multiple sequence alignments . all tools allow the user to load tens of thousands of sequences to edit / analyze and compare them . ivistmsa implemented a divide - and - conquer ( dnc ) approach to calculate efficiently consensus / conserved sequences , identity matrix , sum - of - pairs score ( sps ) , colum score ( cs ) , and conserved regions of two alignments . sps of a column is calculated as the ratio of the sum of scores of all pairs of residues in every column of test alignment by sum of scores of all pairs of residues in the similar column of reference alignment . to calculate sps of the entire alignment , sum of sum of scores of each column of the test alignment cs is computed as the ratio of matched columns ( between test and reference alignments ) by the number of considered columns in the test alignment . the sequence file with 807 sequences named as bba0039.tfa available in  rv100  folder enclosed in a zipped file named as this data set was replicated to generate an alignment with 1,614 sequences , which was replicated to generate 3,228 sequences , and so on . replication was done in order to save time and avoid from constructing big alignments from an msa method . all tools of ivistmsa ( fig . 1 ) are written in java programming language . xml was used for saving the state of the work performed in msapad and msa comparator . a computing machine having core i7 3.34-ghz processor and 8-gb ram was used to write and analyze ivistmsa . most of the tools of ivistmsa use dnc approach for performing efficient computations on msas . the dnc approach has been implemented using a power feature of multithreading provided by java programming language . dnc approach divides an alignment horizontally into subalignments , and java threads are generated for each fragment . all java threads return results to the main thread , which computes the final value . msapad uses dnc approach to compute consensus , conserved sequence(s ) , and distance matrix to construct a phylogenetic tree . step 1 calculates number of subalignments for a given msa . by default , each alignment is divided into two subalignments . step 2 creates a list / array for holding size of each sub - msa . step 3 calculates the size of the first subalignment and stores it into the first position of the list . step 5 subtracts one from the  submsas  variable because up to now , the size of the first sub - msa has been calculated . variable is used to store the size of subalignments at correct positions in the list . figure 2b shows the overall process of dividing msa into sub - msas , computations performed by each thread , and the final computation by the main thread . the alignment - rendering model has played a very important role to display , edit , and analyze very big alignments efficiently . the model sits between the alignment and its viewer and manages efficiently various types of manipulations on the alignments . these manipulations include finding a residue , all types of editing features provided by ivistmsa , changing color schemes , and loading the alignment itself . abstracttablemodel  class provided as part of the swing library of the java programming language . results showed that , in contrast to all other alignment editors written in java programming language such as strap , jalview , and base - by - base , it can load more than 400% big alignments . msapad allows the user to save the alignment , find a single residue or sequence name , add the sequence at the start or end of an alignment , add a sequence before or after a selected sequence , and move a sequence up or down ( fig . 4b ) . it can find consensus and conserved sequence / regions of an alignment of 120-mb size in just 27 seconds . it allows the user to calculate a phylogenetic tree using neighbor joining% identity and blosum 62 matrix . the tree is drawn using archaeopteryx , which is an open - source software tool for displaying and analyzing a phylogenetic tree . archaeopteryx and jmol are embedded to view and analyze phylogenetic trees and protein 3d chemical structures , respectively ( fig . its version 1.0.2 is already published in life science journal.16 data structures used in mqatv1.0.2 were improved , and it is now more efficient than qscore program ( http://www.drive5.com/qscore/ ) and fastsp as well . the new version of mqat allows the user to visualize the conserved and nonconserved regions of the selected alignments from the main window of mqat . figure 5a displays the main window of mqat , which allows the user to select an alignment . results showed that the msa comparator can display conserved and nonconserved regions of two alignments comprising more than 8,000 sequences with a sequence length of 2,696 base pairs in less than 12 seconds . msa reconstruction tool provides graphical interfaces for clustal omega , clustaw2 , mafft , muscle , and biojava implementation for msas . its unique feature is that the user can load multiple sequence files at the same time . now the user does not need to sit before the system and wait for the completion of the process so that the next sequence file may be uploaded . using msa reconstruction tool of ivistmsa interfaces for clustal omega , clustalw2 , mafft , muscle , and biojava can be loaded by clicking the msa reconstruction drop - down list ( fig . fatsa generator can convert clustalw , msf , phylip , pir , gde , mega , and nexus formats of alignments of unlimited size into fasta format . we converted successfully an alignment of msf format comprising 102,102 sequences into fasta format in less than 1 second . the other important feature of this tool is that the user can load alignments of different formats . fasta generator recognizes the format of an alignment automatically and converts it into fasta format . msa i d calculator allows the user to calculate the identity matrix of an alignment . results showed that it can calculate the identity matrix of more than 11,000 sequences with a sequence length of 2,696 base pairs in less than 100 seconds . tree calculation tool calculates a phylogenetic tree using neighbor joining% identity and blosum 62 matrix and permits the user to draw the tree using archaeopteryx , which is an open - source software tool for drawing and analyzing a phylogenetic tree . ivistmsa is a suite of seven interactive visual tools to generate , view , edit , and analyze msas . presently , a lot of msa editing and analyzing tools are available . the popular and widely used tools include jalview,5 seaview,9 mega,10 strap,8 pfaat,17 base - by - base,12 and cinema.11 jalview , strap , pfaat , and base - by - base are java programs , whereas seaview , mega , and cinema are written in c++ language . results showed that msapad loaded more than 50,000 sequences with a sequence length of 2,696 base pairs , whereas other alignment editors written in java programming language could load less than 12,000 sequences only . alignment editors written in c++ language loaded more sequences than msapad , but their editing features are not user friendly . msapad allows the user to edit a residue at its own position without opening a new interface , whereas all other tools allow editing of an alignment in a new interface . msapad provides the feature to insert a sequence at any location of an alignment , whereas most of the available editing tools allow the user to insert a sequence at the end of an alignment . similarly moving a column and sorting an alignment by column are also the unique features of msa - pad . some of the alignment editors such as homed18 and maligned19 are no longer maintained by their authors . several tools such as suite msa,6 sinicview,13 altavist,7 and balibase c program20 are available to compute sps and cs but they can not process more than 1,000 sequences . results showed that the msa comparator was 5,200% efficient as compared to balibase c program . it calculated conserved regions of two alignments comprising more than 8,000 sequences with a sequence length of 2,696 base pairs in less than 12 seconds , whereas suitemsa allowed the user to compute conserved regions of two alignments consisting of less than 1,000 sequences . many software tools such as strap , suitemsa , pfaat , and seaview provide graphical user interfaces for generating msas , but they do not allow the user to load several sequence files simultaneously . msa reconstruction tool allows the user to load several sequence files at a time to align them one by one using clustal omega , clustalw2 , mafft , muscle , or biojava . lot of tools and web servers such as alter21 and readseq22 are available for converting formats of msas , but they process alignments comprising a few thousand sequences . fasta generator allows the user to convert seven msa formats ( clustalw , msf , phylip , pir , gde , and nexus ) into fasta format . it can generate an identity matrix of more than 500 sequences with sequence length of 2,696 base pairs , which was very time consuming . since , sias is a web application , it was also not a good tool for calculating identity matrix of more than 1,000 sequences with a sequence length of 2,696 base pairs . msa i d calculator is an efficient tool , which calculated the identity matrix of more than 11,000 sequences with a sequence length of 2,696 base pairs in less than 100 seconds . comparison indicates that msapad , msa comparator , fasta generator , and msa i d calculator are more efficient than other similar tools available in the market for multiple sequence alignments . msapad allows the user to edit and analyze 409% more data than jalview , strap , cinema , and base - by - base . msa comparator ( mqat version 2.0.1 ) allows the user to visualize consistent and inconsistent regions of reference and test alignments of more than 21-mb size in less than 12 seconds . msa reconstruction tool allows a user to upload several sequence files through the graphical user interfaces of clustal omega , clustalw2 , mafft , and muscle , and then align them one by one . fasta generator converts the other seven formats of alignments of unlimited size into fasta format in a few seconds . msa i d calculator is a tool that allows a user to calculate the identity matrix of more than 11,000 sequences with a sequence length of 2,696 base pairs in less than 100 seconds . tree and distance matrix calculation tools generate phylogenetic tree and distance matrix , respectively , using the neighbor joining% identity and blosum 62 matrix . ivistmsa allows scientists to view , edit , interpret , and analyze very big alignments . project name : ivistmsa project home page : http://ivistmsa.com/ operating system(s ) : tested on windows but it should run on other platforms as well programming language : java 1.7 execution requirements : jdk1.7 or higher any restrictions to use by nonacademics : none
ivistmsa is a software package of seven graphical tools for multiple sequence alignments . 
 msapad is an editing and analysis tool . 
 it can load 409% more data than jalview , strap , cinema , and base - by - base . 
 msa comparator allows the user to visualize consistent and inconsistent regions of reference and test alignments of more than 21-mb size in less than 12 seconds . 
 msa comparator is 5,200% efficient and more than 40% efficient as compared to balibase c program and fastsp , respectively . 
 msa reconstruction tool provides graphical user interfaces for four popular aligners and allows the user to load several sequence files at a time . 
 fasta generator converts seven formats of alignments of unlimited size into fasta format in a few seconds . 
 msa i d calculator calculates identity matrix of more than 11,000 sequences with a sequence length of 2,696 base pairs in less than 100 seconds . 
 tree and distance matrix calculation tools generate phylogenetic tree and distance matrix , respectively , using neighbor joining% identity and blosum 62 matrix .