maxillary constriction can cause posterior crossbite , dental crowding , and abnormal muscular function . in the orthodontic field , maxillary expansion is performed to correct maxillary transverse constriction and the tooth axes of the posterior teeth , alleviate dental crowding , and establish a favorable maxillomandibular relationship.1 however , according to melsen,2 responses to orthopedic maxillary expansion differ depending on the age and maturation of the patient . the interlock of the midpalatal suture increases as it matures , making skeletal expansion even more difficult . as a result , expansion of a matured maxilla leads to a tipping movement of the teeth , which increases the risk of relapse because the extent of dental expansion is greater than that of orthopedic expansion . it is possible to expand the maxilla using a removable or fixed appliance in young patients because the structure of the suture is simple . however , with increased complexity of the suture , as occurs in adults , different treatment methodssuch as surgically assisted rapid palatal expansion ( sarpe)should be used.1 however , sarpe can cause postoperative side effects , discomfort , and psychological and economic burdens on patients.3 recently , a new method that can load orthopedic pressure directly to the bone ( i.e. , miniscrew - assisted rapid palatal expansion , marpe ) has been introduced in clinical practice . marpe also can be considered at a certain level of maturation.45 generally , various indices of maturation have been used to make decisions about treatment timing and method in orthodontics . an evaluation of skeletal age is one of the most important pieces of information in the diagnosis of growing children . the hand and wrist method ( hwm)67 and cervical vertebrae method ( cvm)8 are the most commonly used maturation indices . hellman 's index also is a popular method for assessing dental age.9 it is now possible to observe images of the midpalatal suture by using cone - beam computed tomography ( cbct ) ; this is impossible with conventional radiography . angelieri et al.10 suggested that maturation of the midpalatal suture can be classified into five stages ( stages a , they found it possible to minimize the failure of rapid maxillary expansion in adolescent and young adult patients . however , it is impossible to obtain routine cbct radiography of every patient , and especially those without any diagnostic need ( e.g. , impacted tooth , cyst , and skeletal asymmetry ) . the aim of this study was to classify the maturation degree based on the morphology of the midpalatal suture by using cbct images and to investigate relationships with conventional developmental age indices ( indices of maturation ) . in doing so , we sought to determine whether using conventional developmental age indices can predict the morphology of the midpalatal suture and be used for maxillary expansion treatment planning . before the study commenced , we estimated the sample size needed to reach statistical significance . a power analysis with g*power 3.1.9.2 ( universitt dsseldorf , germany ) showed that 93 subjects would be needed for a statistical power of more than 85% to detect significant differences with a 0.5 effect size and a significance level of  = 0.05 ( actual power = 0.851 ; critical chi - square = 31.41 ; noncentrality parameter  = 23.25 ) . institutional review board approval was granted by the wonkwang university daejeon dental hospital ( daejeon , korea ) to conduct this study ( irb no . w1404/004 - 001 ) . from august 2009 to february 2014 , patients between the ages of 7 and 20 years who visited the department of orthodontics , wonkwang university sanbon dental hospital ( gunpo , korea ) for orthodontic treatment and who underwent cbct were selected . among 319 patients in this group , 99 patients without any exclusion factors the average ages of the sample groups were 14.3  3.27 years ( ages 818 years ) and 13.56  3.12 years ( ages 620 years ) for male and female subjects , respectively . we collected data that had been obtained from each patient for orthodontic diagnosis including cbct images for assessment of the midpalatal suture , hand - wrist and cephalometric radiographs for bone age , and panoramic radiographs for dental age . the exclusion criteria were as follows : 
 any experience with orthodontic treatmentdisease or medicine intake affecting bone metabolismomission of any diagnostic data , including cbct imagespoor - quality images that were difficult to distinguish ( e.g. , blurry images)more than 2 months ' difference between the dates when cbct and other radiographs were acquired 
  any experience with orthodontic treatment disease or medicine intake affecting bone metabolism omission of any diagnostic data , including cbct images poor - quality images that were difficult to distinguish ( e.g. , blurry images ) more than 2 months ' difference between the dates when cbct and other radiographs were acquired cbct ( pax - zenith3d ; vatech korea ind . co. , gyeong - gido , korea ) images were taken using the following parameters : 105 kvp , 6.2 mas , 1524 second scan time , 0.2 and 0.3 mm voxel sizes , and field - of - view , 16 cm  14 cm . the images were converted to digital imaging and communication in medicine ( dicom ) format . dicom files were reconstructed into a three - dimensional image by multiplanar reformatting and volume rendering using imaging software ( invivodental 5.0 ; anatomage , san jose , ca , usa ) . cephalometric radiographs , panoramic radiographs , and hand - wrist radiographs were evaluated by using an exclusive imaging program ( piviewstar ; infinitt , seoul , korea ) . a screen capture of every slide and image was taken and saved in jpeg file format for this study . every slide and image was arranged on a black background and assessed on a 27-inch high - resolution ( 1,920  1,018 pixel ) monitor using a viewer program ( acdsee pro 6.2 ; acd systems international inc . , there was no modification made on the monitor or to the saved images , such as changing the brightness or contrast . to standardize the cbct images , the vertical line of the cursor ( green line ) was matched to the axis of the palatal plane line ( anterior nasal spine - posterior nasal spine ; ans - pns ) on an axial view ( figure 1a ) . at the same time , on a coronal view , the vertical line of the cursor was matched to the nasal septum , and the horizontal line of the cursor ( orange line ) was oriented parallel to the palatal plane ( figure 1b ) . to facilitate observation of the axial cross - sectional planar view of the midpalatal suture , it was established that the horizontal line of the cursor would intersect the middle of the palate in the sagittal plane ( figure 1c ) . to evaluate the morphology of the midpalatal suture more accurately than in a previous study,10 a new axial cross - sectional plane was established as follows : four points that divide the ans - pns into fifths in the midsagittal plane were defined as point a , point b , point c , and point d , starting at the nearest pns point ( figure 1d ) . a vertical line was drawn from point a to the horizontal line of the cursor , and the points at which the extension of the vertical line met the upper and lower borders of the palatal bone were defined as point a ' and point a " , respectively . the midpoint of point a ' and point a " was defined as point a. point b and point c were defined using the same method ( figure 1d ) . point d was excluded from measurement because images of the nasopalatine canal and midpalatal suture showed a high tendency to overlap . the horizontal line of the cursor was matched to a virtual line connecting point a , point b , and point c ( figure 1d ) . the horizontal cross - sectional image that showed the midpalatal suture most evidently and seemed longest was selected by moving the horizontal line up and down in a 1-mm range . if it was impossible to find a line connecting all three points , the shape of the palate was considered a curve , and two horizontal cross - sectional images going through point a  point b and point b  point c were observed . cbct images of the midpalatal suture were assessed on an axial plane view from stage a to stage e according to the classification scheme of angelieri et al.10 the morphology of the midpalatal suture was regarded as an indicator of maturation , similar to a previous study . additionally , we defined " cbct stage " as the determined morphological stage of the midpalatal suture . to make the assessment more accurate , maturation of the midpalatal suture was reconfirmed on a horizontal cross - sectional image by additionally investigating its morphology and fusion on a coronal cross - sectional planar view and on volume - rendered images . when the coronal cross - sectional image was observed as showing fusion at all three points ( point a  point c ) , it was categorized as stage e. if only some points showed fusion , it was judged as stage d. if the suture was open at all three points , it was judged as " before stage c " ; if there were two high radiopaque lines with low density in the middle of the suture , or if the suture was not fused , it was considered as stage c. if only some points showed " stage c conditions , " it was judged as stage b. if there was no stage c condition and a mixture of opacity was observed at all three points , or if only one weak radiopaque line was observed , it was judged as stage a. then , the volume - rendered computed tomography image was clipped with a minimum unit of a 5-mm thickness , including the midpalatal suture . opacity , brightness , and contrast were adjusted to maximize visibility on an axial plane view . among the rendering modes in the software , only three modes ( i.e. , gray scale mode , inverse mode , and soft tissue 2 mode ) that clearly visualized the midpalatal suture were used . final maturation of the midpalatal suture was determined by reconfirming the result from each volume rendering mode ( figure 2 ) . the skeletal maturation indicator ( smi ) proposed by fishman67 the cvm was used on cephalometric radiographs , as suggested by hassel and farman.8 dental age was assessed by applying the hellman 's index to a panoramic radiograph.9 the chronological age and sex of each patient were investigated . after measurement , 30 samples were selected randomly from the same patient group after 2 months and re - assessed with the same method . the intra - class correlation coefficient ( icc ) was calculated to test the reliability of the cbct stage , and developmental age indices were determined by one investigator . icc values were 0.995 ( p < 0.05 ) for cbct stage , 0.996 ( p < 0.05 ) for the hwm , 0.991 ( p < 0.05 ) for the cvm , and 0.992 ( p < 0.05 ) for hellman 's index . to observe correlations between cbct stages and each maturation index , a crosstab analysis by contingency coefficients was performed to determine associations between cbct stages and each maturation index . assessment was performed by using gamma (  ) and kendall 's tau - b ( -b ) as association measures . before the study commenced , we estimated the sample size needed to reach statistical significance . a power analysis with g*power 3.1.9.2 ( universitt dsseldorf , germany ) showed that 93 subjects would be needed for a statistical power of more than 85% to detect significant differences with a 0.5 effect size and a significance level of  = 0.05 ( actual power = 0.851 ; critical chi - square = 31.41 ; noncentrality parameter  = 23.25 ) . institutional review board approval was granted by the wonkwang university daejeon dental hospital ( daejeon , korea ) to conduct this study ( irb no . w1404/004 - 001 ) . from august 2009 to february 2014 , patients between the ages of 7 and 20 years who visited the department of orthodontics , wonkwang university sanbon dental hospital ( gunpo , korea ) for orthodontic treatment and who underwent cbct were selected . among 319 patients in this group , 99 patients without any exclusion factors the average ages of the sample groups were 14.3  3.27 years ( ages 818 years ) and 13.56  3.12 years ( ages 620 years ) for male and female subjects , respectively . we collected data that had been obtained from each patient for orthodontic diagnosis including cbct images for assessment of the midpalatal suture , hand - wrist and cephalometric radiographs for bone age , and panoramic radiographs for dental age . the exclusion criteria were as follows : 
 any experience with orthodontic treatmentdisease or medicine intake affecting bone metabolismomission of any diagnostic data , including cbct imagespoor - quality images that were difficult to distinguish ( e.g. , blurry images)more than 2 months ' difference between the dates when cbct and other radiographs were acquired 
  any experience with orthodontic treatment disease or medicine intake affecting bone metabolism omission of any diagnostic data , including cbct images poor - quality images that were difficult to distinguish ( e.g. , blurry images ) more than 2 months ' difference between the dates when cbct and other radiographs were acquired cbct ( pax - zenith3d ; vatech korea ind . co. , gyeong - gido , korea ) images were taken using the following parameters : 105 kvp , 6.2 mas , 1524 second scan time , 0.2 and 0.3 mm voxel sizes , and field - of - view , 16 cm  14 cm . the images were converted to digital imaging and communication in medicine ( dicom ) format . dicom files were reconstructed into a three - dimensional image by multiplanar reformatting and volume rendering using imaging software ( invivodental 5.0 ; anatomage , san jose , ca , usa ) . cephalometric radiographs , panoramic radiographs , and hand - wrist radiographs were evaluated by using an exclusive imaging program ( piviewstar ; infinitt , seoul , korea ) . a screen capture of every slide and image was taken and saved in jpeg file format for this study . every slide and image was arranged on a black background and assessed on a 27-inch high - resolution ( 1,920  1,018 pixel ) monitor using a viewer program ( acdsee pro 6.2 ; acd systems international inc . , there was no modification made on the monitor or to the saved images , such as changing the brightness or contrast . to standardize the cbct images , head reorientation was performed . after making the position indicator visible , the vertical line of the cursor ( green line ) was matched to the axis of the palatal plane line ( anterior nasal spine - posterior nasal spine ; ans - pns ) on an axial view ( figure 1a ) . at the same time , on a coronal view , the vertical line of the cursor was matched to the nasal septum , and the horizontal line of the cursor ( orange line ) was oriented parallel to the palatal plane ( figure 1b ) . to facilitate observation of the axial cross - sectional planar view of the midpalatal suture , it was established that the horizontal line of the cursor would intersect the middle of the palate in the sagittal plane ( figure 1c ) . to evaluate the morphology of the midpalatal suture more accurately than in a previous study,10 a new axial cross - sectional plane was established as follows : four points that divide the ans - pns into fifths in the midsagittal plane were defined as point a , point b , point c , and point d , starting at the nearest pns point ( figure 1d ) . a vertical line was drawn from point a to the horizontal line of the cursor , and the points at which the extension of the vertical line met the upper and lower borders of the palatal bone were defined as point a ' and point a " , respectively . the midpoint of point a ' and point a " was defined as point a. point b and point c were defined using the same method ( figure 1d ) . point d was excluded from measurement because images of the nasopalatine canal and midpalatal suture showed a high tendency to overlap . the horizontal line of the cursor was matched to a virtual line connecting point a , point b , and point c ( figure 1d ) . the horizontal cross - sectional image that showed the midpalatal suture most evidently and seemed longest was selected by moving the horizontal line up and down in a 1-mm range . if it was impossible to find a line connecting all three points , the shape of the palate was considered a curve , and two horizontal cross - sectional images going through point a  point b and point b  point c were observed . cbct images of the midpalatal suture were assessed on an axial plane view from stage a to stage e according to the classification scheme of angelieri et al.10 the morphology of the midpalatal suture was regarded as an indicator of maturation , similar to a previous study . additionally , we defined " cbct stage " as the determined morphological stage of the midpalatal suture . to make the assessment more accurate , maturation of the midpalatal suture was reconfirmed on a horizontal cross - sectional image by additionally investigating its morphology and fusion on a coronal cross - sectional planar view and on volume - rendered images . when the coronal cross - sectional image was observed as showing fusion at all three points ( point a  point c ) , it was categorized as stage e. if only some points showed fusion , it was judged as stage d. if the suture was open at all three points , it was judged as " before stage c " ; if there were two high radiopaque lines with low density in the middle of the suture , or if the suture was not fused , it was considered as stage c. if only some points showed " stage c conditions , " it was judged as stage b. if there was no stage c condition and a mixture of opacity was observed at all three points , or if only one weak radiopaque line was observed , it was judged as stage a. then , the volume - rendered computed tomography image was clipped with a minimum unit of a 5-mm thickness , including the midpalatal suture . opacity , brightness , and contrast were adjusted to maximize visibility on an axial plane view . among the rendering modes in the software , only three modes ( i.e. , gray scale mode , inverse mode , and soft tissue 2 mode ) that clearly visualized the midpalatal suture were used . final maturation of the midpalatal suture was determined by reconfirming the result from each volume rendering mode ( figure 2 ) . the skeletal maturation indicator ( smi ) proposed by fishman67 was used on hand - wrist radiographs to evaluate bone age . the cvm was used on cephalometric radiographs , as suggested by hassel and farman.8 dental age was assessed by applying the hellman 's index to a panoramic radiograph.9 the chronological age and sex of each patient were investigated . 22.0 ( ibm co. , armonk , ny , usa ) . the distribution and percentage of each measurement and age were calculated . after measurement , 30 samples were selected randomly from the same patient group after 2 months and re - assessed with the same method . the intra - class correlation coefficient ( icc ) was calculated to test the reliability of the cbct stage , and developmental age indices were determined by one investigator . icc values were 0.995 ( p < 0.05 ) for cbct stage , 0.996 ( p < 0.05 ) for the hwm , 0.991 ( p < 0.05 ) for the cvm , and 0.992 ( p < 0.05 ) for hellman 's index . to observe correlations between cbct stages and each maturation index , a crosstab analysis by contingency coefficients was performed to determine associations between cbct stages and each maturation index . assessment was performed by using gamma (  ) and kendall 's tau - b ( -b ) as association measures . the distribution of the total sample and the sexes related to each developmental age index according to cbct stage ( maturation of the midpalatal suture ) are presented in tables 2 , 3 , 4 , 5 , 6 . correlations between cbct stage and developmental age indices ( hwm , cvm , and hellman 's dental age ) or chronological age were investigated . the hwm and cbct stage showed an especially strong correlation ( 0.904 ) and the cvm and cbct stage showed a strong correlation ( 0.874 ) . correlations between cbct stage and hellman 's index for chronological age were relatively weak ( 0.777 and 0.774 , respectively ) . in male subjects , a strong correlation was observed between cbct stage and the hwm ( 0.857 ) . the cvm also showed a strong correlation ( 0.813 ) , and this result was similar in female subjects ( 0.887 and 0.862 , respectively ) . the results of the crosstab analysis between cbct stage and the hwm , cvm , hellman 's index , and chronological age are presented in table 8 . crosstab analysis by contingency coefficients showed that the hwm and cvm had the highest  and kendall 's -b values . when compared , the hwm and cvm both showed significantly high values , but the hwm showed a slightly higher value (  = 0.924 > 0.905 , kendall 's -b = 0.087 > 0.784 ) . the association between hellman 's index and chronological age also reached a significant level , but the contingency coefficient values were lower than those for the hwm and cvm (  = 0.809 and 0.741 ; and kendall 's -b = 0.673 and 0.635 , respectively ) . the crosstab analysis according to sex also showed that the hwm and cvm were significantly higher , while hellman 's index and chronological age were relatively lower ( table 8) . the distribution of the total sample and the sexes related to each developmental age index according to cbct stage ( maturation of the midpalatal suture ) are presented in tables 2 , 3 , 4 , 5 , 6 . correlations between cbct stage and developmental age indices ( hwm , cvm , and hellman 's dental age ) or chronological age were investigated . the hwm and cbct stage showed an especially strong correlation ( 0.904 ) and the cvm and cbct stage showed a strong correlation ( 0.874 ) . correlations between cbct stage and hellman 's index for chronological age were relatively weak ( 0.777 and 0.774 , respectively ) . in male subjects , a strong correlation was observed between cbct stage and the hwm ( 0.857 ) . the cvm also showed a strong correlation ( 0.813 ) , and this result was similar in female subjects ( 0.887 and 0.862 , respectively ) . the results of the crosstab analysis between cbct stage and the hwm , cvm , hellman 's index , and chronological age are presented in table 8 . crosstab analysis by contingency coefficients showed that the hwm and cvm had the highest  and kendall 's -b values . when compared , the hwm and cvm both showed significantly high values , but the hwm showed a slightly higher value (  = 0.924 > 0.905 , kendall 's -b = 0.087 > 0.784 ) . the association between hellman 's index and chronological age also reached a significant level , but the contingency coefficient values were lower than those for the hwm and cvm (  = 0.809 and 0.741 ; and kendall 's -b = 0.673 and 0.635 , respectively ) . the crosstab analysis according to sex also showed that the hwm and cvm were significantly higher , while hellman 's index and chronological age were relatively lower ( table 8) . there have been many attempts to determine whether surgical procedures are necessary to expand the maxilla . sarpe has been recommended by timms and vero11 for patients aged 25 years and older and by epker and wolford12 for those aged 16 years and older . moreover , many other studies recommended various ages from 14 to 20 years and older.131415 however , accurate clinical guidelines regarding treatment timing for maxillary expansion are not available . additionally , existing studies have shortcomings in that they suggested appropriate treatment timing in chronological age ; however , it is generally known that chronological age is not a precise index in predicting skeletal maturation , and these studies did not assess the midpalatal suture itself.1617 therefore , the aim of this study was to investigate the relationship between various developmental age indices including skeletal age and the morphology of the midpalatal suture . to evaluate the morphology of the midpalatal suture according to maturation , cbct images were assessed by conventional methods.1018 however , conventional methods have limitations , including the possibility of the images appearing different depending on the position of the cross - sectional slice . if the cross - sectional slice is not positioned properly in the middle of the midpalatal suture , the practitioner can misjudge the cbct stage . therefore , in this study , it was established that the cross - section slice would intersect the middle of the palate , and maturation of the midpalatal suture also was evaluated based on a coronal cross - sectional planar view and on various volume - rendered images ( figures 1 and 2 ) . angelieri et al.10 reported that stage a was observed mostly in the early childhood period from 5 to 11 years of age ( four of five subjects ) , and stage b was observed mostly up to 13 years of age ( 50 of 57 subjects ) . stage a was observed mostly in ages 5 to 10 years , and stage b was observed mostly in ages 10 to 12 years ( table 6 ) . in this study , fusion of the midpalatal suture below age 11 was not seen ; compared with angelieri et al.,10 stage c had a relatively more dense distribution from 9 to 14 years of age in the current study , probably because of differences in the experimental method and race ( table 6 ) . the female subject sample was distributed somewhat more toward the upper side in table 6 than the male sample at the same cbct stage in this study , meaning that maturation occurred earlier in female subjects than in male subjects ( tables 3 , 4 , 6 ) . these findings were similar to those in a prior study.10 this coincides with the fact that pubescent growth begins and is completed 2 years earlier in females than in males.19 however , because the number of samples in this study was not sufficient and the female sample showing stage d or e was larger than in the male sample , it was difficult to conclude if there was a difference between the sexes . the correlation analysis in this study showed statistical significance for all index values ( table 7 ) . among them , the hwm and cvm showed strong correlations with cbct stage ( 0.904 and 0.874 , respectively ) , while chronological age and hellman 's dental age showed relatively weak correlations ( 0.774 and 0.777 , respectively ) . this result was similar to the findings of other studies showing strong correlations between facial skeletal growth and skeletal age.1920 a difference is that previous studies evaluated facial size by linear growth of the mandible , but the current study evaluated maturation of the midpalatal suture . because of statistical weakness , it was impossible to compare relative usability among each index with values from the correlation analysis . thus , a crosstab analysis using a contingency coefficient was performed additionally ( table 8).21 when the crosstab analysis was performed with cbct stages , the hwm (  = 0.924 , kendall 's -b = 0.807 ) and cvm (  = 0.905 , kendall 's -b = 0.784 ) showed higher values than chronological age (  = 0.741 , kendall 's -b = 0.635 ) and hellman 's dental age (  = 0.809 , kendall 's -b = 0.673 ) . this means that maturation based on the morphology of the midpalatal suture was more consistent with skeletal age than with chronological age or dental age . this further demonstrates that when predicting the morphology of the midpalatal suture , it can be expected that skeletal age ( hwm and cvm ) is a more useful index than either chronological or dental age . there has been no study comparing developmental age indices and cbct findings in the manner of the current study ; only studies about the clinical usability and predictability of the hwm and cvm have been performed.8172223 as presented in table 8 , the crosstab analysis between the hwm and cbct stage showed higher values than the analysis between the cvm and cbct stage in both sexes . this illustrates that the hwm is more suitable for predicting maturation based on the morphology of the midpalatal suture , reflecting a result similar to those of other studies . recently , beit et al.24 and mellion et al.25 reported that the cvm offered no advantage over chronological age in assessing skeletal age or predicting the pubertal growth spurt . however , mellion et al.25 reported that although the hwm is not accurate , it nonetheless is useful in predicting the maximum growth period and in assessing skeletal age because of the repeatability of measurements and least inter - observer error . beit et al.24 also suggested that the hwm has more reproducibility , sensitivity , and accuracy in predicting the maximum growth period because the sesamoid bone serves as a certain landmark . it is notable that before stage 6 of the hwm , stage d or e ( i.e. , fusion of the suture ) was not observed in either the male group or female group ( table 3 ) . there was no fusion before stage 3 of the cvm in the female group or before stage 4 in the male group ( table 4 ) . only when stage 10 or 11 of the hwm and stage 5 or 6 of the cvm appeared did stage e become evident , meaning that there was total fusion of the suture ( tables 3 and 4 ) therefore , nonsurgical maxillary expansion may be recommended before stage 6 in the smi and before stage 3 in the cvm , and a surgical approach may be considered after these stages are recognized ; direct assessment of the midpalatal suture using cbct may be recommended . an issue that must be considered in maxillary expansion is other anatomical structures that resist expansion force . it is well known that resisting anatomical structures include not only the midpalatal suture , but also the zygomaticotemporal suture , zygomaticofrontal suture , and zygomaticomaxillary suture , among others.262728 only the midpalatal suture was considered in this study . another issue that must be considered in this study is that the morphology on the radiographic image can differ from the actual structure of the midpalatal suture itself.29 histological assessment and micro computed tomography may be needed to evaluate maturation of the midpalatal suture with greater accuracy.10 in this study , we evaluated maturation stage based on the morphology of the midpalatal suture on cbct images , and investigated correlations and associations between the maturation stage of the midpalatal suture and developmental age indices . among developmental age indices , the hwm and cvm showed strong correlations and high associations with the maturation stage of the midpalatal suture on cbct images , meaning that these methods can be used to speculate on the maturation of the midpalatal suture according to its morphology .
objectivethe purpose of this study was to determine whether predicting maturation of the midpalatal suture is possible by classifying its morphology on cone - beam computed tomography ( cbct ) images and to investigate relationships with other developmental age indices.methodsthe morphology of the midpalatal suture was assessed by using cbct images of 99 patients . 
 axial plane images of the midpalatal suture were classified into five stages according to the classification scheme . to make the assessment more accurate , 
 the morphology and fusion of the midpalatal suture were additionally investigated on coronal cross - sectional planar images and volume - rendered images . 
 bone age was evaluated using the hand and wrist method ( hwm ) and cervical vertebrae method ( cvm ) ; dental age ( hellman 's index ) , sex , and chronological age were also assessed . to evaluate relationships among variables , spearman 's rho rank test was performed along with crosstabs using contingency coefficients.resultsthe hwm and 
 cvm showed strong correlations with the maturation stage of the midpalatal suture , while other indices showed relatively weak correlations ( p < 0.01 ) . through crosstabs , 
 the hwm and cvm showed high association values with cbct stage ; the hwm demonstrated slightly higher values ( p < 0.0001 ) . 
 based on the hwm , the midpalatal suture was not fused until stage 6 in both sexes.conclusionsamong developmental age indices , the hwm and cvm showed strong correlations and high associations , suggesting that they can be useful in assessing maturation of the midpalatal suture .