fried seeds subjected to grinding by adding sufficient water to it for 30 - 45 min . the mixture was filtered through muslin cloth and pea nut milk is separated . solvent { ethanol : acetone ( 1:1 ) } was added to the milk and then subjected to refrigeration for 30 min . indomethacin was dissolved in chloroform and bio - polymer solution was prepared by adding water to it . the drug solution was added to bio - material solution and mixed by magnetic stirrer until emulsion is formed . release of drug through egg shell membrane : egg shell membrane was separated using con . the egg shell membrane attached to the tube . measured quantity of micro particles 5 ml solution was withdrawal and 5 ml buffer solution was added to make the volume . same procedure was repeated for different formulated films of aceclofenac with phosphate buffer ( ph-7.4 ) . the dissolution studies were perform and subjecting it for dissolution for 3 hrs and analyze the samples by uv spectrophotometer at 320 nm . % cummulative release vs time in ph 1.2 % cummulative release vs time in ph 7.4 fried seeds subjected to grinding by adding sufficient water to it for 30 - 45 min . the mixture was filtered through muslin cloth and pea nut milk is separated . solvent { ethanol : acetone ( 1:1 ) } was added to the milk and then subjected to refrigeration for 30 min . indomethacin was dissolved in chloroform and bio - polymer solution was prepared by adding water to it . the drug solution was added to bio - material solution and mixed by magnetic stirrer until emulsion is formed . release of drug through egg shell membrane : egg shell membrane was separated using con . the egg shell membrane attached to the tube . measured quantity of micro particles 5 ml solution was withdrawal and 5 ml buffer solution was added to make the volume . same procedure was repeated for different formulated films of aceclofenac with phosphate buffer ( ph-7.4 ) . the dissolution studies were perform and subjecting it for dissolution for 3 hrs and analyze the samples by uv spectrophotometer at 320 nm . % cummulative release vs time in ph 1.2 % cummulative release vs time in ph 7.4 five different formulations were formulated using various proportion of bio loaded sustained release bio - micro particles was performed . it had t50% and t 80% of 1.56 hrs and 2.45 hr formulation fg-5 shows percentage yield t50% and t80% of 1.62 hrs and 2.61 hrs at hence these two formulations are the best among the five prepared formulations .
indomethacin microparticles prepared by extraction of bio polymer from arachis hypogen . in vitro drug release study 
 was carried out through egg shell membrane for 3 hrs and analyzed sample by uv spectroscopy at 320 nm . 
 a novel bio membrane from arachis hypogen was isolated by simplify economical process . 
 the % yield was found to be 80% and particle range was 3265 m .