medical and paramedical staff , particularly in service in emergency planning , are frequently exposed to situations of great physical and psychological stress . an increasing number of articles in the literature have focused on the development of psychic and somatic symptoms in the health workforce . emergency departments , in fact , may be challenging because of frequent unpredictability of daily work cases , coping with the acute phase of most disorders , and frequent facing of patient and their families expectations in unexpected critical cases / situations . this has induced an increasing number of researchers to investigate work - related psychological disorders , such as burnout syndrome ( bos ) and , more recently , posttraumatic stress disorder ( ptsd).15 burnout denotes an occupational health concept of emotional and physical exhaustion , depersonalization , and decreased personal accomplishment . bos has been defined as a  state of physical , emotional and mental exhaustion caused by long - term involvement in emotional demanding situations  experienced in the workplace.2 this definition highlights bos as a psychological state resulting from prolonged exposure to job stressors . prevalence rates range from 10% to 50% , depending on profession , assessment tools , and population.2,3,69 literature has increasingly investigated ptsd prevalence rates , both in its full and subthreshold forms , as well as posttraumatic stress symptoms , among these same populations , upon the acknowledgment of the stressful characteristics of this kind of employment.2,7,9 emergency workers , in fact , must cope with a variety of duty - related stressors , including traumatic incident exposure . accordingly , the diagnostic and statistical manual of mental disorders fifth edition ( dsm-5 ) itself explicates , among the changes introduced in ptsd diagnostic criteria , that  repeated or extreme indirect exposure to aversive details of the event(s ) , usually in the course of professional duties ( eg , first responders , collecting body parts ; professionals repeatedly exposed to details of child abuse)10  can be considered as a stressor ( criterion a4 ) . studies aimed at assessing ptsd prevalence rates among emergency unit operators reported values between 10% and 17%.9,1114 a study on emergency rescuers serving in london15 reported work stress to be the most important morbidity factor , with a ptsd diagnosis reported in 15% of the individuals exposed to traumatic events while on duty . clohessy and ehlers,13 in a study on a sample of 56 ambulance service workers of the oxfordshire ambulance nhs trust , found a ptsd prevalence rate of 21% according to dsm - iv16 criteria . the most reported symptoms were intrusive memories ( 49% ) , irritability ( 51% ) , and insomnia ( 47% ) . more recently , mealer et al2 reported a ptsd diagnosis in 18% of 332 university hospital nurses in the usa , while ptsd symptoms were reported in 22% of the same sample . accordingly , an extensive systematic review17 of all empirical articles regarding emergency medical responders and conceptual literature on the constructs of interest in other related populations reported exposure to traumatic events to be between 80% and 100% , and rates of ptsd higher than 20% . in this same review , a modification of the stress process model is suggested to explain the relationships among occupational stress exposure , ptsd , and high risk of alcohol and other drug use . most recently , fjeldheim et al5 reported 94% of 131 south african university paramedic trainees to have directly experienced trauma , with 16% meeting ptsd criteria and 7% chronic perceived stress , suggesting the need for efficient , ongoing screening of ptsd symptomatology in trauma - exposed high - risk groups . recently , some authors have explored ptsd prevalence rates among emergency workers not only in its full - blown manifestation but also in partial and subthreshold forms.13,1820 clohessy and ehlers,13 in a study on 56 ambulance service workers in the united kingdom , found that 21% of responders met dsm - iii - text revised criteria for ptsd , while 22% met screening criteria for psychiatric symptoms . mealer et al2 reported ptsd symptoms in 22% of the nurses operating in a university hospital , while genest et al22 evidenced the onset of intrusive symptoms in emergency operators who attempted basic life support . in the framework of an international italian spectrum - project  , between researchers of the universities of pisa ( italy ) , pittsburgh ( usa ) , columbia ( new york , usa ) , and california at san diego ( usa ) , established to develop and test assessment instruments for assessment of the spectrum of clinical features associated with dsm psychiatric disorders , some researchers developed a multidimensional spectrum approach to ptsd . the spectrum model proposed highlights the significance of isolated symptoms and subthreshold symptom clusters that accompany each disorder classified in the dsm , and may follow , or be manifested , in concurrence with the main disorder.23 accordingly , the trauma and loss spectrum self - report ( tals - sr)24,25 explores the spectrum related to ptsd upon a multidimensional approach across three major dimensions : that of the traumatic event , including the so - called low - magnitude events ; that of the acute reaction ; and that of the symptomatological clusters including maladaptive behaviors . most recently , data on the ptsd symptomatological prevalence data according to dsm-5 criteria among survivors to natural and human - made disasters have been reported by some of researchers.26,27 little data are yet available on dsm-5 ptsd prevalence rates among operators of the emergency units in europe , particularly in italy . the aim of this study was to assess dsm-5 ptsd , as well as posttraumatic stress spectrum symptomatology , among the staff working in the emergency units of a major university hospital in central italy . a further aim of this study was to examine the impact of ptsd and posttraumatic stress symptoms on the work and social functioning of the same persons . a total sample of 110 persons employed , at the time of enrollment , at the emergency units ( emergency medicine and emergency room ) of the  azienda ospedaliero - universitaria pisana ( aoup )  was included in this study . the ethics committee of the university of pisa approved all the procedures of evaluation and recruitment . suitable candidates provided written informed consent after receiving an exhaustive description of the study and after having the opportunity to ask any questions in reference to the study . all persons were assessed by means of the tals - sr25 for posttraumatic stress spectrum symptoms and the work and social adjustment scale ( wsas28 ) for work and social functioning . the tals - sr is a questionnaire developed for assessing posttraumatic stress spectrum symptoms.24,25 it includes 116 items exploring the lifetime experience of a range of losses and/or traumatic events and lifetime symptoms , behaviors , and personal characteristics that might represent manifestations and/or risk factors for the development of a stress response syndrome . the instrument is organized into nine domains including loss events ( i ) , grief reactions ( ii ) , potentially traumatic events ( iii ) , reactions to losses or upsetting events ( iv ) , re - experiencing ( v ) , avoidance and numbing ( vi ) , maladaptive coping ( vii ) , arousal ( viii ) , and personal characteristics / risk factors ( ix ) . the responses to the items are coded in a dichotomous way ( yes / no ) , and domain scores are obtained by counting the number of positive answers . in accordance with the aims of the study , all participants were asked to report symptoms related to work - related trauma exposure . according to previous studies29,30 a dsm-5 diagnosis of ptsd was assessed by using the following matching between dsm-5 symptoms criteria and tals - sr items : 
 criterion b ( b1 = 80 ; b2 = 77 ; b3 = 79 ; b4 = 78 ; b5 = 81);criterion c ( c1 = 86 ; c2 = 87 and/or 88 and/or 89);criterion d ( d1 = 90 ; d2 = 95 ; d3 = 85 ; d4 = 96 ; d5 = 91 ; d6 = 93 ; d7 = 92 ) ; andcriterion e ( e1 = 108 ; e2 = 99 and/or 100 and/or 102 ; and/or 103 and/or 104 ; e3 = 106 ; e4 = 107 ; e5 = 105 ; e6 = 109 ) . criterion b ( b1 = 80 ; b2 = 77 ; b3 = 79 ; b4 = 78 ; b5 = 81 ) ; criterion c ( c1 = 86 ; c2 = 87 and/or 88 and/or 89 ) ; criterion d ( d1 = 90 ; d2 = 95 ; d3 = 85 ; d4 = 96 ; d5 = 91 ; d6 = 93 ; d7 = 92 ) ; and criterion e ( e1 = 108 ; e2 = 99 and/or 100 and/or 102 ; and/or 103 and/or 104 ; e3 = 106 ; e4 = 107 ; e5 = 105 ; e6 = 109 ) . due to the sample characteristics , the wsas is a test used to evaluate and measure the work and social adjustment . it includes five items that assess the individual s ability to perform the activities of everyday life and how these are affected in the week prior to the assessment . the second item assesses the ability to cope with household chores such as cleaning the house , looking after the children , and doing the shopping . the third item assesses private recreational activities carried out by the patient , such as going to the cinema , visiting museums , and reading . the fourth and fifth items investigate the family interaction and relationship : in particular , the fourth item investigates the social activities carried out exclusively with people who are not part of the family and includes activities such as parties , tours of pleasure , going clubbing , or going on romantic dates . the fifth item analyzes only the relations with family members with whom the person lives , and whether any problems of the subject under examination have interfered with this type of relationship . each of the five items is rated on a nine - point scale ranging from 0 ( not at all ) to 8 ( severe interference ) , so that the total scores are between 0 and 40 . to compare the groups , we used the chi - square test ( or fisher when necessary ) in the case of categorical variables , student s t - test for quantitative variables with gaussian distribution , and kruskal  wallis and mann  whitney tests for the ones with no gaussian distribution ( see the total score wsas and the total numbers of ptsd criteria satisfied ) . to study the relation between the total wsas scores and the total numbers of ptsd criteria satisfied , we calculated spearman s correlation coefficient . all the processing statistics were conducted using the statistical package for social science ( spss inc . , a total sample of 110 persons employed , at the time of enrollment , at the emergency units ( emergency medicine and emergency room ) of the  azienda ospedaliero - universitaria pisana ( aoup )  was included in this study . the ethics committee of the university of pisa approved all the procedures of evaluation and recruitment . suitable candidates provided written informed consent after receiving an exhaustive description of the study and after having the opportunity to ask any questions in reference to the study . all persons were assessed by means of the tals - sr25 for posttraumatic stress spectrum symptoms and the work and social adjustment scale ( wsas28 ) for work and social functioning . the tals - sr is a questionnaire developed for assessing posttraumatic stress spectrum symptoms.24,25 it includes 116 items exploring the lifetime experience of a range of losses and/or traumatic events and lifetime symptoms , behaviors , and personal characteristics that might represent manifestations and/or risk factors for the development of a stress response syndrome . the instrument is organized into nine domains including loss events ( i ) , grief reactions ( ii ) , potentially traumatic events ( iii ) , reactions to losses or upsetting events ( iv ) , re - experiencing ( v ) , avoidance and numbing ( vi ) , maladaptive coping ( vii ) , arousal ( viii ) , and personal characteristics / risk factors ( ix ) . the responses to the items are coded in a dichotomous way ( yes / no ) , and domain scores are obtained by counting the number of positive answers . in accordance with the aims of the study , all participants were asked to report symptoms related to work - related trauma exposure . according to previous studies29,30 a dsm-5 diagnosis of ptsd was assessed by using the following matching between dsm-5 symptoms criteria and tals - sr items : 
 criterion b ( b1 = 80 ; b2 = 77 ; b3 = 79 ; b4 = 78 ; b5 = 81);criterion c ( c1 = 86 ; c2 = 87 and/or 88 and/or 89);criterion d ( d1 = 90 ; d2 = 95 ; d3 = 85 ; d4 = 96 ; d5 = 91 ; d6 = 93 ; d7 = 92 ) ; andcriterion e ( e1 = 108 ; e2 = 99 and/or 100 and/or 102 ; and/or 103 and/or 104 ; e3 = 106 ; e4 = 107 ; e5 = 105 ; e6 = 109 ) . criterion b ( b1 = 80 ; b2 = 77 ; b3 = 79 ; b4 = 78 ; b5 = 81 ) ; criterion c ( c1 = 86 ; c2 = 87 and/or 88 and/or 89 ) ; criterion d ( d1 = 90 ; d2 = 95 ; d3 = 85 ; d4 = 96 ; d5 = 91 ; d6 = 93 ; d7 = 92 ) ; and criterion e ( e1 = 108 ; e2 = 99 and/or 100 and/or 102 ; and/or 103 and/or 104 ; e3 = 106 ; e4 = 107 ; e5 = 105 ; e6 = 109 ) . due to the sample characteristics , the wsas is a test used to evaluate and measure the work and social adjustment . it includes five items that assess the individual s ability to perform the activities of everyday life and how these are affected in the week prior to the assessment . the second item assesses the ability to cope with household chores such as cleaning the house , looking after the children , and doing the shopping . the third item assesses private recreational activities carried out by the patient , such as going to the cinema , visiting museums , and reading . the fourth and fifth items investigate the family interaction and relationship : in particular , the fourth item investigates the social activities carried out exclusively with people who are not part of the family and includes activities such as parties , tours of pleasure , going clubbing , or going on romantic dates . the fifth item analyzes only the relations with family members with whom the person lives , and whether any problems of the subject under examination have interfered with this type of relationship . each of the five items is rated on a nine - point scale ranging from 0 ( not at all ) to 8 ( severe interference ) , so that the total scores are between 0 and 40 . to compare the groups , we used the chi - square test ( or fisher when necessary ) in the case of categorical variables , student s t - test for quantitative variables with gaussian distribution , and kruskal  wallis and mann  whitney tests for the ones with no gaussian distribution ( see the total score wsas and the total numbers of ptsd criteria satisfied ) . to study the relation between the total wsas scores and the total numbers of ptsd criteria satisfied , we calculated spearman s correlation coefficient . all the processing statistics were conducted using the statistical package for social science ( spss inc . , chicago , il , usa ) , version 22 . complete data were available for 83 ( 75.5% ) persons because the remaining persons gave partial response or no response at all . of the 83 persons included , 30 ( 36.1% ) were males and 53 ( 63.9% ) were females , with a mean age ( sd ) of 40.78.5 years . in particular , among all the responders , 15 ( 18.1% ) were doctors , 51 ( 61.4% ) were nurses , and 17 ( 20.5% ) were health - care assistants . further , 59 ( 71.1% ) had a bachelor s degree or an advanced degree ( these included not only doctors but also nurses , particularly younger ones who hold a university degree ) , while the remaining 24 ( 28.9% ) were not graduates ( including both some nurses and all of the health - care assistants ) . the department in which persons were employed was evaluated for the health - care assistants and the nursing staff only . all the doctors , in fact , were subject to rotation and served alternately in both departments . among the health - care assistants and nurses , 26 ( 38.2% ) were serving in the department of emergency medicine , while 42 ( 61.8% ) were working in the emergency room . clinical and demographic characteristics of the study sample are reported in table 1 . with regard to the prevalence of ptsd , 13 persons ( 15.7% , confidence interval [ ci ] 99% : [ 5.4%26.0% ] ) met dsm-5 criteria for the diagnosis of the disorder : one doctor ( 6.7% ) , four nurses ( 23.5% ) , and eight health - care assistants ( 15.7% ) . of these , 3 were males ( 10.0% ) and 10 were females ( 18.9% ) ; 5 were below the age of 40 years ( 12.2% ) and 8 over the age of 40 years ( 19.0 ) . concerning the education level , seven persons with ptsd had a bachelor s degree ( 11.9% ) , while six had a lower level of education ( 25.0% ) . as far as the comparisons of the mean ranks of the total wsas scores are concerned ( mean  sd , mean rank ) , females reported significantly higher scores than males ( 7.748.16 vs 4.375.79 , p=0.022 ) ( table 2 ) . when correlating the total number of ptsd criterion symptoms satisfied ( corresponding to the total number of tals - sr items corresponding to dsm-5 diagnostic criteria ) and the total wsas scores , we found a moderate / strong significant correlation among health - care assistants ( r=0.565 , p=0.018 ) and nongraduates ( r=0.428 , p=0.037 ) . further , a moderate significant correlation was found among women ( r=0.310 , p=0.024 ) ( table 3 ) . finally , nongraduates had significantly higher tals - sr domain iv ( reaction to potentially traumatic events ) mean scores compared to those of graduates ( 6.463.37 vs 4.813.04 , p=0.033 ) . further , a significantly higher proportion of the former category reported at least one maladaptive item on the tals - sr compared to the latter category ( 7 [ 29.2% ] vs 6 [ 10.2% ] , p=0.045 ) . to the best of our knowledge , this is the first study exploring dsm-5 ptsd prevalence rates , as well as posttraumatic stress spectrum symptoms , in a sample of italian emergency service workers of a major university hospital . our results show a ptsd prevalence of 15.7% , with higher , despite not significant , percentages among nurses and health - care assistants , women , older persons and nongraduates , as well as in those operating in the emergency room . nongraduates also reported significantly higher mean scores on the tals - sr domain iv , exploring the acute reaction to potentially traumatic events , as well as a significantly higher proportion of persons presenting at least one maladaptive behavior , with respect to operators with graduate degrees . among women , significantly higher scores on the wsas also emerged compared to men . when correlating the total number of ptsd criterion symptoms satisfied and the total wsas scores , a strong significant correlation emerged in health - care assistants and nongraduates , while a moderate significant correlation was found in women . the ptsd rate reported in the study sample is remarkable if compared to the recently published ptsd prevalence in the italian general population of 2.3% , diagnosed in accordance with dsm - iv - text revised criteria31 in the framework of the european study of the epidemiology of mental disorders ( esemed).32,33 although caution has to be adopted because the two studies utilized different methods in the sampling and the diagnostic criteria adopted , and thus data can not be directly compared , a possible higher vulnerability to the disorder can be seen in this professional category in italy . if we consider our sample , which enrolled all the staff employed at the aoup , as being possibly representative of italian emergency operators , it is important to highlight that we reported a ci 99% far from the 2.3% of de girolamo et al,32 and thus the ptsd rates could be considered potentially significantly different ( p<0.01 ) . in previous reports , some researchers explored symptomatological ptsd prevalence rates assessed by means of the tals - sr in accordance with either dsm - iv - tr or dsm-5 criteria in the general population exposed to a massive earthquake , reporting substantial correspondence.29,30 this may indirectly corroborate a substantial increase in ptsd rates among emergency workers with respect to the general population in italy . noticeably , our results are also in line with previous reports conducted on rescuers and emergency workers across europe where ptsd prevalence rates ranging between 10% and 21% have been reported.9,1113,34 clohessy and ehlers,13 exploring a sample of paramedics and emergency medical technicians in the uk , showed that 21% of enrolled persons were affected by ptsd as diagnosed using both dsm - iii and dsm - iv . it is also important to highlight that despite the fact that most of the studies involved nurses and paramedics , only scant data are available on the medical staff . maia and ribeiro,35 exploring 59 among nurses and medical staff from the national institute of medical emergency in the north of portugal , reported high exposure to events evaluated as traumatic and low prevalence of ptsd . it is important to note that most of the studies reporting higher ptsd prevalence rates are those conducted on paramedics and nursing staff , with our results showing lower , albeit not significant , rates in doctors compared to the other workers who are in line with these findings . however , caution should be adopted when interpreting our data , as a potential explanation for our findings would be that undergraduate emergency workers typically begin to work with trauma at younger ages than nurses and doctors do . there is evidence , in fact , that younger individuals are at higher risk of developing ptsd from the same trauma as opposed to older peers36 and that the more one is exposed to trauma , the higher is his / her risk of developing ptsd ; thus , we may argue that having started working at an earlier age makes undergraduate workers more globally exposed to trauma than their colleagues . a third potential bias that should be taken into account in larger samples is related to the reasons for choosing emergency care professions ; it is in fact more likely that these may be chosen by individuals with traumatic backgrounds , thus affecting the rates of possible complex ptsd . there is agreement on the fact that ptsd affects females about twice as much as males , also in italian samples , with more severe symptomatology.26,33,3741 however , studies exploring the possible confounding role of other risk factors , including work - related training and education , reported conflicting results.42,43 some authors , in fact , highlighted that differences between the sexes may be compensated for by adequate professional training , as shown in rescue workers , police agents , or firefighters , particularly in the united states.4345 our results seem to be in line with these reports despite needing to be corroborated in larger samples as they might be affected by the fact that a greater percentage of women reported lower education . similarly , our data are in line with studies reporting a relationship between education level and ptsd . in this regard , epidemiological data from the esemed study in italy reported significantly higher ptsd rates in persons with lower instruction levels.33 as previously mentioned , our results also highlighted significantly higher scores on the tals - sr domain iv , exploring symptoms of acute reaction to trauma , and a significantly higher proportion of persons presenting at least one maladaptive behavior , with respect to operators with a graduate degree . increasing literature has documented the relationship between acute distress reaction and ptsd , highlighting ptsd as the ideal candidate for secondary prevention programs.46,47 in this regard , maia and ribeiro35 found peritraumatic dissociation and distress symptoms to be the only predictors of ptsd symptoms among 59 nurses and medical staff from the national institute of medical emergency in the north of portugal . maladaptive behaviors , including suicidal behaviors and substance use , fully entered the dsm-5 diagnositc criteria for ptsd and were repeatedly associated with higher severity of the disorder.4852 fjeldheim et al5 reported a rate of 23% of alcohol abuse in south african university paramedic trainees who directly experienced trauma . our data as a whole seem to thus suggest an increased severity of posttraumatic stress reaction in emergency workers with lower educational levels . consistently , we also found a strong significant correlation between the total number of ptsd symptoms and impairment in work and social adjustment in health - care assistants , nongraduates , and women . some important limitations of the study should be borne in mind when interpreting our results . the most important is related to the limited sample size that may impact on the generalizability of our results . however , we underline the novelty of this study , which , to the best of our knowledge , is the first in the literature to report on ptsd and posttraumatic stress spectrum in emergency workers in italy . second is the need of a standardized interview such as the structured clinical interview for dsm disorders to demonstrate the prevalence of ptsd suggested from our symptomatological data . third , we may argue the fact that most severely avoidant ptsd cases may have not been enrolled because this same symptomatology prevented them from fulfilling the instruments . fourth , we used a self - report instrument , instead of the rating of the clinician , in order to detect ptsd symptoms and also make the diagnosis and be influenced by co - occurring events . nevertheless , the use of tals - sr allowed us to explore dsm-5 criterion symptoms . fifth , as already mentioned in our first study , there is the lack of information on the presence of axis i psychiatric comorbidities . despite the limitations mentioned above , this report suggests emergency health professionals to be at high risk for posttraumatic stress spectrum and related impairments in work and social adjustment . the increased vulnerability reported in specific subgroups , particularly women and operators with lower levels of education , should be further investigated in larger samples , with the aim to help identify specific education and training interventions for prevention programs .
the diagnostic and statistical manual of mental disorders fifth edition ( dsm-5 ) has recently recognized a particular risk for posttraumatic stress disorder ( ptsd ) among first responders ( criterion a4 ) , acknowledging emergency units as stressful places of employment . 
 little data is yet available on dsm-5 among emergency health operators . 
 the aim of this study was to assess dsm-5 symptomatological ptsd and posttraumatic stress spectrum , as well as their impact on work and social functioning , in the emergency staff of a major university hospital in italy . 
 one hundred and ten subjects ( doctors , nurses , and health - care assistants ) were recruited at the emergency unit of the azienda ospedaliero - universitaria pisana ( italy ) and assessed by the trauma and loss spectrum - self report ( tals - sr ) and work and social adjustment scale ( wsas ) . a 15.7% dsm-5 symptomatological ptsd prevalence rate was found . 
 nongraduated persons reported significantly higher tals - sr domain iv ( reaction to loss or traumatic events ) scores and a significantly higher proportion of individuals presenting at least one maladaptive behavior ( tals - sr domain vii ) , with respect to graduate ones . 
 women reported significantly higher wsas scores . 
 significant correlations emerged between ptsd symptoms and wsas total scores among health - care assistants , nongraduates and women . 
 our results showed emergency workers to be at risk for posttraumatic stress spectrum and related work and social impairment , particularly among women and nongraduated subjects .