the advent of regenerative approaches in contemporary periodontics has increased patient 's treatment options and enhanced the long - term prognosis of many teeth that have advanced periodontal destruction . preliminary experimental studies have shown that nanosized ceramics may represent a promising class of bone graft substitutes due to their improved osseointegrative properties . accordingly , a synthetic nanocrystalline hydroxyapatite ( ncha ) bone graft has been introduced for augmentation procedures in intrabony defects . a special feature of nanostructured materials is an extremely high number of molecules on the surface of material . when ncha was used as a bone graft substitute , rapid healing of critical size defects was observed in animal experiments and in human applications . ncha has been used in the treatment of metaphyseal fractures in orthopedic surgery , ridge augmentation , and peri - implantitis lesions . various collagen barriers of mammalian origin , such as bovine and porcine , have been used successfully for guided tissue regeneration guided tissue regeneration ( gtr ) in human intrabony and furcation defect and gingival recession . recently , interest has been developed in non - mammalian collagen sources , primarily fish collagen . a bioresorbable collagen barrier membrane ( periocol ) of fish origin has been developed for gtr applications in human periodontal intrabony and furcation defects . periocol collagen membrane has been used as a sustained release chlorhexidine chip in chronic periodontitis patients and reported to be resorbed after 30 days . however , collagen membrane when dampened by biological fluid results in a poor membrane resistance to collapse , allowing undesirable cell types to enter the secluded wound area . the collapse may be prevented by means of implantation of bone grafts or bone graft substitutes into the defect to support the membrane , preserving its original position . performed in periodontal regeneration has suggested that one of the most predictable techniques in improving clinical attachment level ( cal ) and bone fill is achieved when a combination of graft material and gtr is used.[1315 ] during periodontal therapy , deep intraosseous defects represent a major challenge for the clinician , often requiring open flap debridement ( ofd ) alone or combined with bone - regenerative procedures . various clinical and radiographic studies[9131622 ] demonstrated significant higher probing pocket depth ( ppd ) reduction and greater gain in cal in the combined group compared with ofd group . this study was designed to evaluate the efficacy of ncha bone replacement graft ( sybograf ) in combination with bioresororbable collagen membrane ( periocol ) compared with open flap debridement alone . sixteen systemically healthy patients ( nine male subjects and seven female subjects ) , aged between 25 - 65 years were screened after a detailed clinical and radiographic examination from the out patient department of periodontology , manipal college of dental sciences , mangalore . inclusion of patients either in the test group ( 10 defects ) or control group ( 10 defects ) was determined randomly through the coin flip method . study inclusion criteria were as follows : systemically healthy patients ; presence of atleast one or two radiographically detectable intrabony defects with probing pocket depth 5 mm and radiographic depth of the defect 3 mm ; no antibiotics taken prior to six months of initial examination and did not require antibiotic premedication for any systemic condition ; no periodontal surgery performed in the areas to be treated within the last 12 months ; good level of oral hygiene ( plaque index [ pi ] < 1 ) . subjects with previously implanted materials , natural or synthetic and physical barriers in the selected defects , smokers , pregnant and lactating female subjects ; teeth exhibiting mobility exceeding grade ii and teeth with endodontic involvement were excluded from the study . all 16 patients with 20 intrabony defects ( 9 in maxilla and 11 in mandible ) , following an initial examination , diagnosis and treatment plan were subjected to phase - i therapy , which comprises of full mouth supragingival and subgingival scaling and root planing . a customized acrylic stents was fabricated on study casts for each patient and trimmed to the height of contour of the teeth , and one vertical groove was prepared to standardize the probe angulation and position [ figure 1 ] . all measurements were recorded to nearest millimeter ( mm ) with the help of a unc-15 graduated periodontal probe . clinical parameters ( pi , gingival index ( gi ) , ppd , cal , and gingival recession [ rec ] ) were recorded at baseline and 6 month at the six aspects ( mesio - buccal , mid - buccal , disto - buccal , mesio - lingual , mid lingual , and disto - lingual ) per tooth . only one site representing the deepest point of the ppd was included in the study . measuring the probing depth with stent using the apical margin of the customized acrylic stent as the fixed reference point ( rp ) , the following measurements were recorded at the proximal line angle of the tooth with the associated bony defect . a ) rp to the gingival margin [ gm ] ; b ) rp to the cemento - enamel junction [ cej ] ; and c ) rp to the base of the pocket [ bop ] . the following clinical parameters were recorded using the acrylic stent : a ) ppd = [ rp to bop ]  [ rp to gm ] ; b ) cal = [ rp to bop ]  [ rp to cej ] ; and c ) rec = [ rp to gm ]  [ rp to cej ] . an intra - oral periapical radiograph was taken for each selected site using the long cone paralleling technique with mm grid scale , at baseline [ figure 2 ] and at 6 months [ figure 3 ] post - surgery . all radiographs were digitalized using the digital camera and transferred to the computer as jpeg image to measure the linear radiographic depth ( in mm ) of the defect ( dd ) using software , image j , which was designed by national institute of health ( nih ) for the image analysis . preoperative radiograph postoperative radiograph following clinical data collection , a pre - operative mouth rinse with 10 ml 0.2% chlorhexidine gluconate ( plakil india ) was used by all patients . area subjected to surgery was anesthetized by nerve block / infiltration depending on the surgical site using local anesthesia . full thickness flap was raised buccally and lingually until 1 mm bone was exposed in control group , and 2 - 3 mm in the patients of test group . the osseous defect was debrided of granulation tissue and the root surface was planed to remove plaque and calculus , using hand and ultrasonic scalers until a smooth hard consistency was found [ figure 4 ] . the defect 's architecture had to be confirmed by direct observation and classified based on the number of bony walls present . following surgical debridement , no root bio - modification or osseous resective procedures was attempted . intrabony defect after degranulation in patients selected for test group in addition to ofd , ncha bone replacement grafts having particle size 40 - 50 nm ( sybograf ) was utilized to fill the defects to the most coronal level of the osseous walls and type - i sterile collagen gtr membrane ( periocol ) was adapted over the bone graft extended 2 - 3 mm beyond the margin of the defect apically and mesio - distally and 1 mm below the cej [ figure 5 ] . periocol is an orange - brown colored membrane , measuring a size of 2530 mm , in a sterile double blister pack . the source of collagen in periocol gtr membrane is from the air bladder of fresh water fishes . bone graft gtr membrane placed primary soft tissue closure was done with interrupted sutures using non resorbable 3 - 0 black silk sutures . postoperatively , subjects were prescribed antibiotics ( amoxicillin 500 mg tid for 7 days ) to prevent post - operative infection and analgesics ( ibuprofen 400 mg tid for 5 days ) for pain management . patients were instructed to rinse with 0.2% chlorhexidine gluconate ( twice daily for 4 weeks ) . following surgery , the patients were asked to refrain from tooth brushing , flossing , and interdental cleaning techniques in the treated area for 4 weeks after surgery . at 1 week recall visit , dressing , sutures , and recall appointments were then made at 2 and 4 week for additional follow - up and plaque control . all the patients were recalled at 1 , 3 , and 6 month post - surgery for follow - up . at 6 month recall visit full mouth pi and gi was recorded . at each recall appointment all the statistical analysis was done using computer software package for statistical analysis , spss version 11.5 ( spss inc . , wilcoxon signed rank sum test was used for intragroup comparison in both test and control groups . mann - whitney u test was used for intergroup comparison between test and control groups . all the statistical analysis was done using computer software package for statistical analysis , spss version 11.5 ( spss inc . , wilcoxon signed rank sum test was used for intragroup comparison in both test and control groups . mann - whitney u test was used for intergroup comparison between test and control groups . all 16 patients completed the 6 month follow - up period . configuration and distribution of treated intrabony defects are depicted in table 1 . neither allergic reaction nor suppuration or abscess was observed in any of the patients . subjects and defect characteristics at baseline the pi and gi values ( meansd ) showed non - significant difference at baseline and 6 month between the test and control groups as depicted in table 2 . no statistically significant differences were found between groups for any of the investigated parameter ( ppd , cal , rec , and dd ) at baseline [ table 2 ] . test and control group at baseline and 6 months at six months , the mean ppd reduction was 4.330.50 mm for the test and 3.221.09 mm for the control group [ tables 2 and 3 ] . the wilcoxon signed rank sum test indicated that both the test ( p=0.006 ) and control ( p=0.007 ) groups showed a significantly greater mean ppd reduction at six months . analysis by mann whitney u test demonstrated a statistically significantly ( p=0.01 ) greater reduction in mean ppd favoring the test group , and an additional 1.11 mm ppd reduction was observed in test group [ table 3 ] . comparison of change in clinical and radiographic parameter between test and control group at 6 months statistically significant mean cal gains of 3.780.66 mm and 2.781.09 mm were observed in the test and control groups , respectively , from baseline to 6 month [ tables 2 and 3 ] . there was statistically significantly greater cal gains for the test group ( 3.78 mm ) than the control group ( 2.78 mm ) [ table 3 ] . the magnitude of the observed additional benefit was 1.00 mm in the test group . at six months , the mean increase in rec was 0.550.52 mm and 0.440.52 mm in the test and control groups , respectively [ table 3 ] ; although a statistically significant increase in rec was found in both the groups ( p=0.025 test ; p=0.046 control ) [ table 3 ] . none of the sites treated in both test and control groups resulted in an increase in rec of more than 1 mm . the mean gain in radiographic defect fill was recorded as 2.070.67 mm ( 47.55% ) in test and 0.910.21 mm ( 25.33% ) in control group at 6 month [ tables 2 and 3 ] . statistically significant ( p<0.05 ) gain of radiographic defect fill was recorded in both the test as well as in the control group . at 6 month , statistically significant ( p=0.001 ) greater reduction of radiographic dd was observed in test group compared with the control group [ table 3 ] . there was also an additional 1.16 mm ( 22.22 % ) of radiographic bone fill in the test group [ table 3 ] . bone grafts , their synthetic substitutes , and gtr techniques have been used in an attempt to gain this therapeutic endpoint . the present study was designed to compare the combined effect of bg+gtr ( test group ) with ofd ( control group ) in the treatment of intrabony periodontal defects . a clinically and statistically significant improvement in ppd reduction , cal gain , and radiographic defect fill was observed in both test and control groups at 6 month postoperatively compared to baseline , which favored the test group . in the control group , the mean reduction of ppd was 3.221.09 mm and cal gain was 2.771.09 mm at 6 months follow - up visit . in a study by kilic et al , ofd group showed the mean ppd reduction of 3.17 mm , which was in accordance to the results of the present study and cal gain of 2.1 mm which was slightly lower than the present study . the difference in results of ofd group might be influenced by surgical technique , baseline defect characteristics , and operator skill . the reduction in ppd was slightly higher and the cal gain was in agreement with the previous reported study by kasaj et al , that evaluated the clinical efficacy of ncha paste in intrabony defects and reported ppd reduction of 3.91.2 mm and cal gain of 3.61.6 mm . this slightly higher ppd reduction may be due to the effect of combination technique ( bg+gtr ) used in the present study . kilic et al , demonstrated that the combination of ha collagen bone graft with eptfe membrane resulted in higher ppd reduction ( 5.85 mm ) and greater cal gain ( 3.80 mm ) compared with the test group results of our study . the higher reduction of ppd might be explained by the higher gingival recession in the above study ( 2.00 mm ) compared to 0.55 mm in the present study . it was suggested that intrabony defect configuration influences the results after gtr and larger amounts of cal gain were reported in deep three - wall defects than two- or one - wall defects following gtr treatment . a systematic review concluded that in two - wall intrabony defect models of periodontal regeneration , the additional use of a grafting material gave superior histological results of bone repair to barrier membranes alone . in the present study , configuration of defects treated were 2 and 2-walled , which may have benefited from the combination technique . pocket depth reduction , even though not necessarily a result attributed to regeneration , is a major player in decision making in routine periodontal patient care scenarios . initial pocket depth in the defects treated with bg+gtr in the present study were more than 7 mm , and were reduced to value just slightly above 3 mm depth accepted as manageable by current patient care standard . intergroup comparison of the results at 6 month showed that there was an additional 1.11 mm ppd reduction and 1.00 mm cal gain in the test group . trombelliconcluded that additional effect of the combination treatment ( gtr + bg ) is similar to gtr alone when compared to ofd alone with respect to attachment gain , but results in slightly more ppd reduction and greater gain in hard tissue probing at re - entry surgery . the combined group showed a significantly greater cal gain , lesser alveolar crest reduction , and greater vertical bone gain compared with the gtr alone group . in fact , collagen membranes are characterized by a lack of stiffness when they are dampened by biological fluids . the presence of a physical support under such a material allows the membrane to maintain its position when the flaps are sutured over the defect , exerting pressure onto the membrane itself . it should , however , be pointed out that in the present study the collagen membrane was not fixed by means of bioresorbable sutures or pins . thus , it can not be excluded that the membrane was displaced during flap suturing or the healing process and may have acted only for stabilization of the graft particles . periodontal therapies are usually associated with gingival recession which is of esthetic concern for both patients and clinicians . the rec in the present study showed no statistically significant difference between the test and control sites at baseline and at six months . the increase in rec in present study was lower in both test and control groups compared to the previously reported similar study by sculean et al . the ability of a probe to penetrate into a pocket is related to several factors including probing force and gingival tissue condition . camargo et al , stated that the improvement in clinical parameter can result in gain in attachment ; however , it should be remembered that placement of graft material into the defect may modify gingival tissue consistency and therefore interfere with the penetration of periodontal probe without necessarily having induced any gain in cal . hence , bone fill data derived from surgical re - entry is important to substantiate routine post - operative measurements due to the above mentioned reason . however , it has certain inherent disadvantages like inducing further resorption at the treated site and inability to ascertain the exact histological nature of the hard tissue . the second surgical procedure is also time consuming and may interrupt the regenerative process if healing is still ongoing . zybutz et al , concluded that standardized radiographs reliably and permanently describe the hard tissue changes and thus can serve as substitute for probing to bone or re - entry measurements of bone changes . in the present study , a change of the alveolar bone level was detected radiographically using consecutive pre- and post - operative radiographs . prefabricated film holders may provide projection standardization to a certain degree . in the present study , linear measurement from the crest to the base of the intrabony defect j image  software , which can overcome errors in the linear measurement to a certain degree than manual measurements . in the present study , the radiographic defect depth gain was 2.070.67 mm , which represents radiographic defect depth fill of 47 % at six months in test group . kilic et al , reported 1.55 mm gain of radiographic defect depth in test group , which was slightly lower than the present study . several reports indicate that the bone fill is enhanced by the addition of a graft material to gtr procedures . present study demonstrated additional 1.16 mm ( 22% ) radiographic defect depth fill in the test group compared with the control group over six month period , although complete regeneration was not achieved . wenzel et al , reported no increased bone fill between 6 and 12 months may support the six month radiographic analysis of the present study . question arises whether the radiographically assessed increase in the defect fill in the test group could represent the new bone formation or the presence of residual graft material or both , although ncha was reported to be resorbable . the present study demonstrated statistically significant but slight clinical improvement in cal gain ( 1.00 mm ) in the test group compared with ofd . the most reliable outcome variable for assessing periodontal regeneration is histological analysis ; however , due to ethical consideration and patient management limitation , no histological evidence was obtained to establish proof of periodontal regeneration in present study . combined use of ncha bone graft with collagen membrane resulted in clinically and statistically significant ppd reduction , cal gain and bone fill percentage , compared to ofd alone , in periodontal intrabony defects .
aim : to evaluate the clinical outcome of nanocrystalline hydroxyapatite ( ncha ) bonegraft ( sybograf ) in combination with collagen membrane ( periocol ) compared with open flap debridement ( ofd ) only in the treatment of intrabony periodontal defects.materials and methods : eighteen intrabony defects in 16 systemically healthy patients aged between 25 - 65 years , were randomly assigned to test and control groups . 
 the plaque index , gingival index , probing pocket depth ( ppd ) , clinical attachment level ( cal ) , and gingival recession were recorded at baseline , and were reevaluated at 6 months . 
 in addition to this , radiographic bone fill was assessed using digital software . at the test site ncha bone graft and collagen membrane 
 was placed , whereas at the control site only , ofd was done . 
 recall appointments were made at 7th day , 1st month , 3rd month , and 6th month.results:the data were subjected to statistical analysis using the mann - whitney  u test and wilcoxon signed rank sum test . in the control group , 
 the mean reduction of ppd was 3.221.09 mm and cal gain was 2.781.09 mm . in the test group , the mean ppd reduction of 4.330.5 mm and mean gain in cal was 3.780.66 mm at 6 months . 
 the mean increase in gingival recession was 0.550.72 mm in test and 0.440.52 mm in control group.conclusion:the ncha bone graft in combination with collagen membrane demonstrated better clinical outcomes compared with ofd alone .