honey whose medicinal uses date from ancient times has been lately rediscovered as therapy for wounds . the antimicrobial effect of honey has been reported by a number of workers it is commonly used as a base for ointments and has very successfully been applied in surgical dressings for open wounds and burns to avoid septic infections . the current prevalence of antibiotic - resistant microbial species has led to a re - evaluation of the therapeutic use of ancient remedies , including honey . strong solution of honey or sugar and sugar pastes inhibits microbial growth due to high osmolarity but when used as dressings this action ceases . but it has been traditionally used in treatment of burn wound infection in rural india and the study has shown that it is superior to silver sulfadiazine . indiscriminate use of antibiotics has led to the emergence of drug - resistant strains which have a significant impact on patient 's morbidity and mortality . to date , there are many publications on studies performed both in vitro and in vivo on the therapeutic properties of manuka honey , and have confirmed its activity against a wide range of medically important bacteria . honey is produced from many floral sources and its antimicrobial activity varies markedly with its origin and processing.[911 ] this variation can be due to difference in the enzymatic action and in the presence of additional antibacterial components derived from the floral source . as the potential role for honey as a topical agent to manage surgical site or wound infections is increasingly acknowledged other honeys need to be assessed and evaluated . the present study was to evaluate the antimicrobial activity of honey native to india at various concentrations against pseudomonas aeruginosa causing wound infection and its comparison with antibiotics and dettol . a total of 50 strains of p. aeruginosa isolated from different types of wound infection including burns wound were used in the study . the antibiotic - sensitivity patterns of the isolates were studied by kirby bauer 's disc diffusion method using commercially available antibiotic discs ( hi - media labs , mumbai india ) gentamicin ( 10 g ) , amikacin ( 30 g ) , ceftazidime ( 30 g ) , ciprofloxacin ( 5 g ) , netillin ( 30 g ) , cefotaxime ( 30 g ) , piperacillin ( 100 g ) , and imipenem ( 10 g ) as per the clinical and laboratory standards institute ( clsi ) standards . agmark grade honey ( dabur india , capital overseas ) , a polyfloral honey with yellowish brown color , was used in the study . it had 100% purity and each 100 g of honey contains 80 g natural sugars , sodium 17 mg , potassium 138 mg , calcium 13 mg , iron 1.5 mg , and phosphorus 5 mg ; sterilized by ultrafiltration and have the floral source from himalayas , nilgiris , and sunderbans of india . the minimum active dilution of honey against p. aeruginosa isolates was determined by agar dilution methods . double strength nutrient agar was used for the study , measured out into 10 ml aliquots and autoclaved . to prepare the plates it was melted and maintained at a temperature of 50 c water - bath until poured . solutions of the honey samples ( at a concentration of 50% v / v ) were prepared in sterile de - ionized water immediately prior to performing an assay . appropriate concentrations of the stock and deionized water ( total 10 ml ) were dispensed aseptically into 10 ml sterile double strength nutrient agar before pouring into petri dishes to produce a dilution series ( 5 - 25% ) from concentrations required in a volume of 20 ml . antibacterial action of honey was compared with dettol , ( reckitt benckiser india ltd , capital overseas ) at a concentration similar to honey which contains an aromatic chemical compound chloroxylenol ( c8h9clo ) ( 4.8% of the total dettol mixture ) , which is the key ingredient exhibiting unique antiseptic property . the turbidity was adjusted to 0.5 mc farland and 10 l of culture was spot inoculated on to the surface of the medium . the plates were incubated at 37c for 24 h before visual assessment of the growth . control plates of plain nutrient agar were included in each susceptibility assay to confirm the viability and density of the cultures . aeruginosa american type culture collection ( atcc ) strain 27853 was inoculated on each plate for comparison . honey was used undiluted and at different dilutions . with each experiment saline control of the organism two milliliters each of undiluted and different dilutions of the honey were taken in sterile test tubes and inoculated with 20 l of broth culture of the test bacterium in an initial concentration of approximately 510 cfu / ml . all the tubes were incubated at 37c and the viable count of bacteria in each tube was determined at an interval of 4 h up to 24 h by the surface plate method . viable count of bacteria in both test and control tubes at each time interval was noted by performing plate culture on nutrient agar without honey . agmark grade honey ( dabur india , capital overseas ) , a polyfloral honey with yellowish brown color , was used in the study . it had 100% purity and each 100 g of honey contains 80 g natural sugars , sodium 17 mg , potassium 138 mg , calcium 13 mg , iron 1.5 mg , and phosphorus 5 mg ; sterilized by ultrafiltration and have the floral source from himalayas , nilgiris , and sunderbans of india . the minimum active dilution of honey against p. aeruginosa isolates was determined by agar dilution methods . double strength nutrient agar was used for the study , measured out into 10 ml aliquots and autoclaved . to prepare the plates it was melted and maintained at a temperature of 50 c water - bath until poured . solutions of the honey samples ( at a concentration of 50% v / v ) were prepared in sterile de - ionized water immediately prior to performing an assay . appropriate concentrations of the stock and deionized water ( total 10 ml ) were dispensed aseptically into 10 ml sterile double strength nutrient agar before pouring into petri dishes to produce a dilution series ( 5 - 25% ) from concentrations required in a volume of 20 ml . antibacterial action of honey was compared with dettol , ( reckitt benckiser india ltd , capital overseas ) at a concentration similar to honey which contains an aromatic chemical compound chloroxylenol ( c8h9clo ) ( 4.8% of the total dettol mixture ) , which is the key ingredient exhibiting unique antiseptic property . the turbidity was adjusted to 0.5 mc farland and 10 l of culture was spot inoculated on to the surface of the medium . the plates were incubated at 37c for 24 h before visual assessment of the growth . control plates of plain nutrient agar were included in each susceptibility assay to confirm the viability and density of the cultures . aeruginosa american type culture collection ( atcc ) strain 27853 was inoculated on each plate for comparison . honey was used undiluted and at different dilutions . with each experiment saline control of the organism two milliliters each of undiluted and different dilutions of the honey were taken in sterile test tubes and inoculated with 20 l of broth culture of the test bacterium in an initial concentration of approximately 510 cfu / ml . all the tubes were incubated at 37c and the viable count of bacteria in each tube was determined at an interval of 4 h up to 24 h by the surface plate method . viable count of bacteria in both test and control tubes at each time interval was noted by performing plate culture on nutrient agar without honey . a total of 50 strains of p. aeruginosa isolated from infected wounds were studied over a period of 2 years . the antibiotic susceptibility patterns of the p. aeruginosa isolates are shown in figure 1 , where the y axis indicates the percentage of the isolates susceptible to various antibiotics plotted on the x axis . multidrug resistance was a common feature among most of these isolates 41 ( 82% ) and the remaining 9 ( 18% ) isolates were pan sensitive . among the antibiotics tested against p. aeruginosa isolates in this study imipenem 50 ( 100% ) being highly effective followed by piperacillin 39 ( 78% ) , amikacin 38 ( 76% ) netillin 32 ( 64% ) , ceftazidime 28 ( 56% ) , gentamicin 23 ( 46% ) , cefotaxime 23 ( 46% ) , and ciprofloxacin 20 ( 40% ) , respectively . percentage of susceptibility pattern to different class of antibiotics to pseudomonas aeruginosa isolates used in the study the effects of honey on all the isolates of p. aeruginosa were studied by the determination of minimum inhibitory concentration ( mic ) , indicating the highest dilution of honey in the culture medium which inhibits the growth of p. aeruginosa isolates . the mic of all the 50 strains ( 100% ) to honey was found to be 20% , of which 19 strains ( 38% ) were having an mic of 15% including the standard strain of p. aeruginosa atcc 27853 . honey concentrations ranging from 5% to 10% were found to be ineffective as shown in figure 2 . the y axis indicates the number susceptible percentage of isolates to honey and dettol at various concentrations ( 5 - 25% ) plotted on the x axis . mic of pseudomonas aeruginosa isolates towards honey and dettol at different concentrations the time kill assay for the bactericidal effect was tested on five isolates of p. aeruginosa so as to understand the time required to kill the bacterial population irrespective of their antibiotic susceptibility patterns as isolates varying in their susceptibility pattern were used . honey at concentrations of 20% , 25% , 50% could bring out complete destruction in 24 h , whereas concentration of honey at 75% and 100% could bring about complete destruction of p. aeruginosa in 12 h [ table 1 ] . the lowest concentration of the honey which showed bactericidal activity was at 20% for all the five isolates tested in time kill assay . the antibacterial activity of honey was concentration dependent for all the 50 isolates tested at a concentration below 20% using agar dilution methods [ figure 2 ] . the antibacterial effect of 50% honey on p. aeruginosa with number of hours and percentage of survival rate was calculated . the average percentages of survival rate for five p. aeruginosa isolates after 4 h , 8 h , 12 h , and 24 h were found to be 22.3 , 5.2 , 1.1 , and 0 respectively as shown in table 2 . time kill assay of honey against the wound isolates of pseudomonas aeruginosa antibacterial effect of 50% honey on p. aeruginosa wound isolates the present study shows the bactericidal activity of honey against p. aeruginosa strains . as honey is considered as a natural antiseptic in the management of wound infections , its efficacy was compared with dettol . it was observed that these organisms were more or less susceptible to honey and dettol at 20% and 10% irrespective to their antibiotic sensitivity patterns . dettol antiseptic liquid contains chloroxylenol which is proved to kill a wide variety of microbes and is widely used for cleansing wound . the antibacterial action is due to disruption of the cell membrane potentials and blocking the production of adenosine triphosphate . the antibacterial effect of honey was concentration dependent and bactericidal effect was observed at concentration of 20% or more for p. aeruginosa isolates tested . since p. aeruginosa are recalcitrant to antibiotic therapy the ability to inhibit test isolates irrespective of their antibiotic sensitivity patterns has important clinical applications . this property may make honey useful in the treatment of drug - resistant infections . in this study , we found that indian origin honey have activity against p. aeruginosa wound isolates comparable to tualang and manuka honey as reported in previous studies . reported that any honey can have equivalent antibacterial activity against some bacteria compared with other pharmaceutical honeys , whereas basson et al . found no such high antimicrobial activity for honeys native to south africa . compared the activity of ulmo90 and manuka honey and reported similar activity against the p. aeruginosa at a concentration of 12.5% , better than the results from our study . results from these studies confirm that honeys from different countries and regions may have wide variations in their antimicrobial activity . it has been shown that honey may have antimicrobial action ranging from lesser than 3% to 50% and higher concentrations . honey has been successfully used in the treatment of surgical wounds , burns wound , and decubitus ulcers . it has also been shown as a good medium to store skin grafts and honey has antileishmanial effect also . high sugar concentration , low ph , hydrogen peroxide generation , proteinaceous compounds , or other unidentified components present in the honey may all provide antimicrobial activity . shrinkage and disruption of the bacteria may be due to its osmotic effect , low ph , and also due to the presence of antibacterial substance such as inhibine . several components may contribute to the non - peroxide activities of honey , such as the presence of methyl syringate and methylglyoxal , which have been extensively studied in leptospermum honeys . besides its antimicrobial properties , honey can clear infection in a number of ways in vivo , like boosting the immune system , anti - inflammatory , and antioxidant activities and via stimulation of cell growth . after reviewing the literature it has been found that apart from mulai et al . , no other indian study has been carried out exclusively on antibacterial activity of honey against p. aeruginosa in vitro . as there is lack of scientific research and documentation , still the medicinal properties of indian honeys further studies on human subjects are required in vivo to understand the efficacy of indian honeys in eliminating bacteria from wounds . as this study presents the findings of in vitro antibacterial activity of honey against planktonic bacteria this should not be related to chronic wound environment where indeed biofilms of p. aeruginosa may be present and the characteristic of bacteria can change ; hence , future studies in this direction will pave the way in establishing the medicinal importance of indian honey against the sessile forms of p. aeruginosa . as honey is easily available in the market and is inexpensive its antibacterial activity was comparable to other medicinal honeys . all strains of p. aeruginosa including both resistant phenotypes and sensitive strains were inhibited at 20% antibacterial honey concentrations in vitro . this intriguing observation may have important clinical implications and could lead to a new approach for treating multidrug resistant p. aeruginosa infected wounds using honey of indian origin .
background : as natural products garner attention in the medical field due to emergence of antibiotic resistant strains of bacteria , honey is valued for its antibacterial activity.objective:fifty strains of pseudomonas aeruginosa isolated from infected wounds were evaluated for their antibacterial action using honey in comparison with different antibiotics and dettol.methodology and results : all the strains were found to be sensitive to honey at a minimum inhibitory concentration of 20% in comparison with dettol at 10% using agar dilution method . in the second step , 
 the time kill assay was performed on five isolates of p. aeruginosa to demonstrate the bactericidal activity of honey at different dilutions of honey ranging from 20% to 100% at regular time intervals . 
 all the isolates of p. aeruginosa tested were killed in 12 - 24 h depending on the dilutions of the honey tested . 
 thus , honey could prevent the growth of p. aeruginosa even if it was diluted by deionized water by fivefolds in vitro . 
 honey had almost uniform bactericidal activity against p. aeruginosa irrespective of their susceptibility to different classes of antibiotics.conclusion:honey which is a natural , non - toxic , and an inexpensive product has activity against the p. aeruginosa isolated from infected wounds may make it an alternative topical choice in the treatment of wound infections .