the beijing genotype is commonly present in russian population and in eurasia as a whole . this lineage , especially bo\w subline is characterized by high - level virulence , , , . for our analysis , we chose the mycobacterium tuberculosis strain b9741 , isolated from a 33-years - old hiv - positive female patient from irkutsk oblast , russia , with firstly diagnosed fibrocavernous tuberculosis , provided by the scfhhrp , irkutsk , russia . this strain was resistant to isoniazid ( inh ) , rifampin ( rif ) , streptomycin ( sm ) and pyrazinamide ( pza ) . genome sequencing was carried out on roche 454 gs junior instrument ( roche , switzerland ) , in the laboratory of bacterial genetics , vigg ras ( moscow , russia ) . all reads were assembled to an initial draft genome : 4,322,170  bp ( total length ) nucleotides at 13-fold coverage using the gs de novo assembler ( version 3.0 ; roche ) ( table 1 ) . the automatic functional annotation results were obtained using ncbi prokaryotic genome annotation pipeline ( pgaap ) ( http://www.ncbi.nlm.nih.gov/genomes/static/pipeline.html ) . the b9741 genome contains 4193 genes ( total ) , 4 rrnas , and 46 trnas . a total of 250 pseudogenes , 3 noncoding rnas ( ncrnas ) , 1 clustered regularly interspaced short palindromic repeats ( crispr ) were predicted using the pgaap . according to housekeeping gene and toxin - antitoxin analysis , , we classified this strain to belong to b0/w beijing lineage . we compared our output sequence with dna sequence of the high - virulent m. tuberculosis w-148 strain which belongs to b0/w cluster of beijing group . in this announcement , we focused on genes , which determine virulence and drug resistance . in addition , we analyzed snps in genes , which are associated with drug resistance to inh , rif , sm , and pza . analysis we provide the comparison of sequenced dna with b0\w dna sequences and revealed the presence of three polymorphisms at virulence genes . in the gene mce3f , which essential for survival of mtbs in macrophages and invasion to the host cells , we have found substitution - d410a , in irtb gene , which encode the part of irtab iron importer - a175 t , . this protein play an essential role for iron homeostasis in stress conditions . and in vapc46 - a38 g , . thus , identified genes will be used for understanding of m. tuberculosis adaptation to patients with low immune level , including hiv  + patients . othis whole genome shotgun ( wgs ) project has been deposited at genbank under the accession lvjj01000000 ( mycobacterium tuberculosis strain b9741 ) . this whole genome shotgun ( wgs ) project has been deposited at genbank under the accession lvjj01000000 ( mycobacterium tuberculosis strain b9741 ) . the beijing genotype is commonly present in russian population and in eurasia as a whole . this lineage , especially bo\w subline is characterized by high - level virulence , , , . for our analysis , we chose the mycobacterium tuberculosis strain b9741 , isolated from a 33-years - old hiv - positive female patient from irkutsk oblast , russia , with firstly diagnosed fibrocavernous tuberculosis , provided by the scfhhrp , irkutsk , russia . this strain was resistant to isoniazid ( inh ) , rifampin ( rif ) , streptomycin ( sm ) and pyrazinamide ( pza ) . genome sequencing was carried out on roche 454 gs junior instrument ( roche , switzerland ) , in the laboratory of bacterial genetics , vigg ras ( moscow , russia ) . all reads were assembled to an initial draft genome : 4,322,170  bp ( total length ) nucleotides at 13-fold coverage using the gs de novo assembler ( version 3.0 ; roche ) ( table 1 ) . the automatic functional annotation results were obtained using ncbi prokaryotic genome annotation pipeline ( pgaap ) ( http://www.ncbi.nlm.nih.gov/genomes/static/pipeline.html ) . the b9741 genome contains 4193 genes ( total ) , 4 rrnas , and 46 trnas . a total of 250 pseudogenes , 3 noncoding rnas ( ncrnas ) , 1 clustered regularly interspaced short palindromic repeats ( crispr ) were predicted using the pgaap . according to housekeeping gene and toxin - antitoxin analysis , , we classified this strain to belong to b0/w beijing lineage . we compared our output sequence with dna sequence of the high - virulent m. tuberculosis w-148 strain which belongs to b0/w cluster of beijing group . in this announcement we developed the catalog of 342 genes determinate virulence , , . for this analysis , in addition , we analyzed snps in genes , which are associated with drug resistance to inh , rif , sm , and pza . we provide the comparison of sequenced dna with b0\w dna sequences and revealed the presence of three polymorphisms at virulence genes . we carried out a deeper analysis of these genes involved in virulence . in the gene mce3f , which essential for survival of mtbs in macrophages and invasion to the host cells , we have found substitution - d410a , in irtb gene , which encode the part of irtab iron importer - a175 t , . this protein play an essential role for iron homeostasis in stress conditions . and in vapc46 - a38 g , . thus , identified genes will be used for understanding of m. tuberculosis adaptation to patients with low immune level , including hiv  + patients . othis whole genome shotgun ( wgs ) project has been deposited at genbank under the accession lvjj01000000 ( mycobacterium tuberculosis strain b9741 ) . this whole genome shotgun ( wgs ) project has been deposited at genbank under the accession lvjj01000000 ( mycobacterium tuberculosis strain b9741 ) . the authors declare that there is no conflict of interests with respect to the work published in this paper . this research did not receive any specific grant from funding agencies in the public , commercial , or not - for - profit sectors .
we report a draft genome sequence of mycobacterium tuberculosis strain b9741 belonging to beijing b0/w lineage isolated from a hiv patient from siberia , russia . 
 this clinical isolate showed mdr phenotype and resistance to isoniazid , rifampin , streptomycin and pyrazinamide . 
 we analyzed snps associated with virulence and resistance . 
 the draft genome sequence and annotation have been deposited at genbank under the accession nz_lvjj00000000 .