the white matter tracts that are the last to myelinate are the first to degenerate . superficial white matter ( swm ) is located beneath the infragranular layer of the cerebral cortex and these tracts are some of the last fibers to myelinate , often occurring in the fourth decade of life . therefore , swm represents a population of tracts that may be most vulnerable to ad . swm consists of u fibers of meynert , crown fibers and merging deep white matter fibers . u fibers originate from layers iii and v of the cortex , exit and follow concave surfaces of a sulcus and re - enter the cortex at distances up to 3 cm . merging fibers originate within the deep white matter , cross other tracts in swm and extend towards the cortex . perhaps due to the complex architecture and significant inter - subject variability of swm , a magnetization transfer ratio magnetic resonance imaging ( mri ) study found evidence of demyelination of swm in ad and an association with lower mini - mental state examination scores . a diffusion tensor mri study found decreased white matter integrity of swm in ad , particularly in the frontal and temporal lobes and associations with lower mini - mental state examination scores . additional imaging studies are needed to characterize the alterations of swm in ad and its cognitive correlates . tractography is an application of diffusion tensor mri that reconstructs white matter tracts based on restricted diffusion of water molecules along myelinated axons . disruption of tracts can be detected as increased mean diffusivity ( md , degree of water diffusion ) , increased axial diffusivity ( axd , largest eigenvalue ) , increased radial diffusivity ( rd , average of the two smaller eigenvalues ) or decreased fractional anisotropy ( fa , directional diffusion ) . a tractography study in healthy older adults demonstrated an association between lower swm fa and lower scores on cognitive testing . earlier tractography studies in ad assessed lobar white matter that likely consisted of both swm and deep white matter , but none assessed only swm . the aim of this study was to use tractography to characterize alterations in swm associated with ad . we used this method to reconstruct each participant 's unique network of swm tracts . since ad has differential effects across cerebral lobes , we separately assessed frontal , occipital , parietal and temporal swm . in each lobe , we compared the diffusion characteristics ( md , axd , rd and fa ) of swm tracts between healthy older adults and persons with ad and correlated it with the performance on neuropsychological testing . we hypothesized that ad would be associated with worse swm diffusion characteristics ( increased md / axd / rd and decreased fa ) . we also hypothesized that worse swm diffusion characteristics would be associated with decreased cognitive function . the study was approved by the queen 's university research ethics board . prior to entering the study , all participants provided written informed consent . the neuropsychological battery consisted of the following cognitive tests : global cognitive function ( montreal cognitive assessment , moca   and mini - mental state examination ) , selective attention ( stroop test ) , processing speed ( trail making test b , trail ) , focused attention ( wechsler memory scale - iii longest span forward ) and working memory ( wechsler memory scale - iii longest span backward and letter number sequencing ) . participants with ad were within the mild stages of the disease , as measured by a moca score of 18/30 . exclusion criteria were the presence of metallic objects , devices or conditions unsafe for mri . twenty - four cognitive normal controls and 16 participants with ad were included in the study . brain imaging was acquired in a single session on a 3 tesla siemens magnetom trio mri system ( siemens medical systems , erlangen , germany ) with the use of a 12-channel head coil . an anatomical scan was acquired with a sagittal t1-weighted 3-dimensional magnetization prepared rapid gradient echo sequence [ field of view ( fov ) 256 mm , spatial resolution 1  1  1 mm , repetition time ( tr ) 1,760 ms , echo time ( te ) 2.2 ms , flip angle 9 , number of slices 176 ] . an axial t2-weighted 2-dimensional fluid - attenuated inversion recovery sequence ( flair ) interleaved scan was acquired ( fov 250 mm , voxel size 1  1  3 mm , tr 9,000 ms , te 79 ms , flip angle 180 , number of slices 40 ) . diffusion tensor imaging ( dti ) data were acquired in 30 directions using a single - shot echo planar imaging sequence with 31 volumes of 60 axial slices ( b - value 1 = 0 s / mm , and b - value 2 = 1,000 s / mm ) , slice thickness 2 mm , tr / te = 7,800/95 ms , fov 256  256 mm and an acquisition matrix of 128  128 , resulting in a resolution of 2  2  2 mm . dti reconstruction was completed with diffusion toolkit 0.5 ( ruopeng wang , van j. wedeen , martinos center for biomedical imaging , massachusetts general hospital , www.trackvis.org ) . tracts were created in the diffusion toolkit by the fiber assignment by continuous tracking ( fact ) method with an angle threshold of 35 . the participant 's t2 flair mri was registered by the affine method using 12 degrees of freedom to the diffusion - weighted image map with slicer 3d 4.1 ( www.slicer.org ) . white matter hyperintensities ( wmh ) were manually segmented on axial t2 flair as described in an earlier study . swm was defined as tracts originating within 5 mm of the cortical surface , and it was manually segmented into frontal , occipital , parietal and temporal lobe swm based on anatomical landmarks [ landmarks are described in ref . ] . within each lobe , region of interests ( rois ) rois were manually traced for each lobe , slice by slice , on the 40 axial t2 flair slices for each patient using the trackvis mouse - controlled interface . frontal , occipital , parietal and temporal lobe swm was segmented by selecting tracts that crossed through the respective lobar rois . the area of roi as well as fa , md , axd and rd of the lobar swm were measured . the fa , md , axd and rd of segmented swm tracts were compared between controls and participants with ad while adjusting for the effects of age and the wmh volume using multiple linear regressions . cognitive scores were not available in all participants , so participants were included in the analysis only when their scores were available ( sample sizes are presented in tables 3 and 4 ) . we presented unstandardized regression coefficients for the associations between cognitive test scores with tracts md , axd , rd and fa and number using multiple linear regressions , adjusting for the effects of age and wmh volume . to account for comparison of the 4 swm lobes , a bonferroni correction was used , and p values < 0.0125 were considered statistically significant . the study was approved by the queen 's university research ethics board . prior to entering the study , all participants provided written informed consent . the neuropsychological battery consisted of the following cognitive tests : global cognitive function ( montreal cognitive assessment , moca   and mini - mental state examination ) , selective attention ( stroop test ) , processing speed ( trail making test b , trail ) , focused attention ( wechsler memory scale - iii longest span forward ) and working memory ( wechsler memory scale - iii longest span backward and letter number sequencing ) . participants with ad were within the mild stages of the disease , as measured by a moca score of 18/30 . exclusion criteria were the presence of metallic objects , devices or conditions unsafe for mri . twenty - four cognitive normal controls and 16 participants with ad were included in the study . brain imaging was acquired in a single session on a 3 tesla siemens magnetom trio mri system ( siemens medical systems , erlangen , germany ) with the use of a 12-channel head coil . an anatomical scan was acquired with a sagittal t1-weighted 3-dimensional magnetization prepared rapid gradient echo sequence [ field of view ( fov ) 256 mm , spatial resolution 1  1  1 mm , repetition time ( tr ) 1,760 ms , echo time ( te ) 2.2 ms , flip angle 9 , number of slices 176 ] . an axial t2-weighted 2-dimensional fluid - attenuated inversion recovery sequence ( flair ) interleaved scan was acquired ( fov 250 mm , voxel size 1  1  3 mm , tr 9,000 ms , te 79 ms , flip angle 180 , number of slices 40 ) . diffusion tensor imaging ( dti ) data were acquired in 30 directions using a single - shot echo planar imaging sequence with 31 volumes of 60 axial slices ( b - value 1 = 0 s / mm , and b - value 2 = 1,000 s / mm ) , slice thickness 2 mm , tr / te = 7,800/95 ms , fov 256  256 mm and an acquisition matrix of 128  128 , resulting in a resolution of 2  2  2 mm . dti reconstruction was completed with diffusion toolkit 0.5 ( ruopeng wang , van j. wedeen , martinos center for biomedical imaging , massachusetts general hospital , www.trackvis.org ) . tracts were created in the diffusion toolkit by the fiber assignment by continuous tracking ( fact ) method with an angle threshold of 35 . the participant 's t2 flair mri was registered by the affine method using 12 degrees of freedom to the diffusion - weighted image map with slicer 3d 4.1 ( www.slicer.org ) . white matter hyperintensities ( wmh ) were manually segmented on axial t2 flair as described in an earlier study . swm was defined as tracts originating within 5 mm of the cortical surface , and it was manually segmented into frontal , occipital , parietal and temporal lobe swm based on anatomical landmarks [ landmarks are described in ref . ] . within each lobe , region of interests ( rois ) were outlined paralleling the cortex within 5 mm of the cortical surface . rois were manually traced for each lobe , slice by slice , on the 40 axial t2 flair slices for each patient using the trackvis mouse - controlled interface . frontal , occipital , parietal and temporal lobe swm was segmented by selecting tracts that crossed through the respective lobar rois . the area of roi as well as fa , md , axd and rd of the lobar swm were measured . the fa , md , axd and rd of segmented swm tracts were compared between controls and participants with ad while adjusting for the effects of age and the wmh volume using multiple linear regressions . cognitive scores were not available in all participants , so participants were included in the analysis only when their scores were available ( sample sizes are presented in tables 3 and 4 ) . we presented unstandardized regression coefficients for the associations between cognitive test scores with tracts md , axd , rd and fa and number using multiple linear regressions , adjusting for the effects of age and wmh volume . to account for comparison of the 4 swm lobes , a bonferroni correction was used , and p values < 0.0125 were considered statistically significant . there was a trend for increased age and increased wmh volume in participants with ad compared to controls ; however , it was not statistically significant ( table 1 ) . as expected , participants with ad had statistically significantly lower scores on all cognitive tests compared to controls ( table 1 ; all tests p < 0.001 ) . compared to controls , the area of swm was smaller in participants with ad ( frontal swm : 5,576  4,556 vs. 1,119  517 , p = 0.00073 ; occipital swm : 1,627  1,211 vs. 316  155 , p = 0.00025 ; parietal swm : 3,652  2,747 vs. 687  161 , p = 0.00015 ; temporal swm : 2,083  1,706 vs. 391  227 , p = 0.00029 ) . compared to controls , in participants with ad , there was increased md ( percentage difference 12% ) , increased axd ( 9% ) and increased rd in temporal swm ( 14% ; table 2 ) . there were no statistically significant differences in the fa of swm between controls and participants with ad ( table 2 ) . among controls , in frontal swm , increased rd and decreased fa were associated with worse moca scores ( table 3 ) . in occipital swm , increased rd was associated with worse moca and trail scores , and decreased fa was associated with worse trail scores . in temporal swm , decreased fa was associated with worse moca scores , and increased md and rd were associated with worse stroop scores . in participants with ad , the fa , md , rd and axd of swm was not associated with the performance on the cognitive tests ( table 4 ) . this is the first tractography study to detect alterations in the swm in ad . compared to controls , the retrogenesis hypothesis predicted that , as late myelinating fibers , swm would be vulnerable to degeneration in ad . in support of the retrogenesis hypothesis , we detected disruption of swm in ad . our results are in agreement with an earlier magnetization transfer ratio imaging study and the dti study that found evidence for disruption of swm in ad . the pattern of swm tract changes observed in this study was similar to earlier reported patterns of cortical neurodegeneration in ad . based on the braak staging system , neurofibrillary degeneration develops in the temporal lobe and spreads to the rest of the neocortex in later stages of the disease . consistent with its involvement in early stages of ad , in this study , we found the largest diffusion abnormalities in the temporal lobe . the large increases in md , axd and rd within the temporal swm ranged from 9 to 14% and are consistent with the 10 - 25% increase reported by an earlier dti study . it is believed that increased md represents tissue atrophy , increased axd represents wallerian degeneration , and increased rd reflects myelin disruption . based on these associations , our study suggests that in ad , there is prominent tissue atrophy , wallerian degeneration and myelin disruption in temporal swm . consistent with earlier studies , there were greater differences in md , axd and rd than in fa between controls and participants with early - stage ad . both axd and rd were increased in participants with ad , which may have attenuated changes in fa in ad . in healthy older adults , decreased global cognitive function was associated with worse diffusion characteristics of swm in several lobes . decreased performance on the moca was associated with increased rd / decreased fa in frontal swm , increased rd in occipital swm and decreased fa in temporal swm . decreased cognitive function in specific cognitive domains was associated with worse diffusion characteristics of swm in specific lobes . decreased selective attention assessed by the stroop test was associated with increased md / rd in temporal swm . decreased processing speed assessed by the trail test was associated with increased rd / decreased fa in occipital swm . cognitive test scores were only available in a subset of participants leading to smaller sample sizes for cognitive correlations . the small sample size may have limited the ability to detect diffusion differences between participants with ad and controls or detect cognitive associations with swm . alternatively , the disruption of swm in ad may have contributed to the lack of cognitive associations for swm in ad . additional limitations of this study are the multiple comparisons , which may have led to false - positive results , and the accuracy of tractography . the complex architecture of swm makes it difficult to follow the course of tracts through crossing or abutting fibers . although some tracts may not have been tracked at all or not correctly , this study and earlier ones have demonstrated that it is possible to perform tractography for swm . with tractography this network of connections between cortical regions assumes an important role in cognition . at the level of cerebral lobes , this study demonstrated that swm has region - specific roles in normal cognition . in healthy persons , tractography of swm can be used to characterize in more detail the relationship between regional cortical connectivity and specific cognitive processes . in ad , tractography of temporal swm may represent a novel biomarker of neuronal injury that may aid in diagnosis and disease monitoring . all procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation ( institutional and national ) and with the helsinki declaration of 1975 , as revised in 2000 .
background / aimssuperficial white matter provides extensive cortico - cortical connections . 
 this tractography study aimed to assess the diffusion characteristics of superficial white matter tracts in alzheimer 's disease.methodsdiffusion tensor 3 t magnetic resonance imaging scans were acquired in 24 controls and 16 participants with alzheimer 's disease . 
 neuropsychological test scores were available in some participants . 
 tractography was performed by the fiber assignment by continuous tracking ( fact ) method . 
 the superficial white matter was manually segmented and divided into frontal , parietal , temporal and occipital lobes . 
 the mean diffusivity ( md ) , radial diffusivity ( rd ) , axial diffusivity ( axd ) and fractional anisotropy ( fa ) of these tracts were compared between controls and participants with alzheimer 's disease and correlated with available cognitive tests while adjusting for age and white matter hyperintensity volume.resultsalzheimer's disease was associated with increased md ( p = 0.0011 ) , increased rd ( p = 0.0019 ) and increased axd ( p = 0.0017 ) in temporal superficial white matter . in controls , superficial white matter was associated with the performance on the montreal cognitive assessment , stroop and trail making test b tests , whereas in alzheimer 's disease patients , it was not associated with the performance on cognitive tests.conclusiontemporal lobe superficial white matter appears to be disrupted in alzheimer 's disease .