serial analysis of gene expression ( sage ) is a powerful method for obtaining comprehensive and quantitative gene expression profiles from cell populations under selected physiological conditions . sage counts polyadenylated transcripts by sequencing a short 14 bp tag at the gene 's 3 end , adjacent to the last restriction site . the tag counts are than archived electronically for future analysis and comparisons . since the first publication introducing sage ( 1 ) , computational tools ( 27 ) and statistical methods ( 820 ) have been developed to correctly perform the analysis of a sage experiment . because sage determines absolute expression levels , comparisons between different sage libraries are relatively easy to perform . for this reason , sage has been selected as the major platform technology for the cancer genome anatomy project ( cgap ) . for 5 years , a large number of sage libraries , generated from diverse cancer and normal tissues in many laboratories , have been accessible via the national center for biotechnology information website ( ) . the sage genie website ( ) was constructed recently and is equipped with additional search and presentation tools ( 7 ) . these libraries were all constructed by using the same anchoring enzyme , thus all yielding tags likely to be 10 bp downstream from the 3 most nlaiii site in the transcript . the aim of most sage studies is to identify genes of interest by comparing the number of specific tags found in two different sage libraries . one of the most attractive applications of transcript profiling is to address the question of expression differences between normal and cancer samples or cancer and metastasis samples in order to define new diagnostic markers and therapeutic targets . over the past few years , several methods have been reported for determining the statistical significance of gene expression difference provided by the sage experiments . most of these methods have been incorporated into public database systems and analysis programs ( 24,68,1013,1820 ) . if within two types of cells y and z , a particular mrna species has unknown respective concentrations y and z and a total of a tags are sequenced from cell type y and b tags from cell type z , and among these , a and b tags , respectively , correspond to the mrna of interest , the question is : what inference may be made about the relative size of the actual concentrations , y and z ? they consider the null hypothesis h0 that y = z , and the alternative that y  z , and derive formulas based upon the observed data for rejecting h0 with various degrees of confidence . if a / a and b / b differ significantly , one rejects h0 , concluding that the expression levels y and z are unequal . we have used their statistical approach and developed a useful and flexible tool ( websage ) that analyse and compare a large number of sage tags . the novelty of our tool compared with those available online ( 3,4 ) consists of the visualization of the results of the comparison of two sage libraries in a scatter plot . one of the libraries is presented on the x - axis and the second one on the y - axis . more than two libraries can not be visualized using our tool unless to pool libraries composed by the sum of all libraries from one group such as cancer group and all libraries from normal group . however , vncio et al . ( 20 ) showed clearly that this summing is an error and proposed a statistical analysis that accounts for the within class variability . in our tool , full sage data are very comprehensively represented in plots , which correspond to one or a set of tags , having the same p - value and the same apparition in two libraries . moreover , websage gives not only the identification but also the function of the gene . to gain insights into the molecular basis for metastasis , we compared the global gene expression profile of metastatic pancreatic cancer with that of primary cancer . the two cell lines were established from a 60-year - old woman with surgically removed pancreatic carcinoma that presented simultaneously metastatic liver lesion ( 21 ) . we isolated total cellular rna from pancreatic cancer cells and metastatic liver cells and constructed each sage library , using sage protocol version 1.0c ( 1 ) . afterwards , tags were extracted from the raw sequence data and repeated ditags were excluded using the sage software ( version 1 ) developed by kinzler and co - workers ( 1 ) . from the two sage libraries 12  090 tags were obtained , enabling us to compare the expression levels of 4807 unique transcripts . at this step , the question of interest is whether one sample has a significant change in expression relative to the other sample for each transcript . over the past few years , several methods have been developed for determining the statistical significance of gene expression difference derived from the sage experiments . zhang et al . ( 17 ) used a simulation approach to determine the probability of obtaining the observed difference and more extreme ones . other statistical approaches of the number of specific tags found in sage can be envisioned . ( 19 ) used a test for differences in tag numbers found in two experimental conditions , seemingly based on poisson distribution statistics . their approach suffers from the disadvantage that it can only be used reliably on tag libraries of similar size . the second approach is to look at the number of copies of a specific mrna per cell as a fraction or proportion of the total number of mrna molecules in that cell . the same proportion ( p ) of specific tags should be present in the sage library of all sequenced tags . in this approach , the number of tags found is binomially distributed because it is the result of the p of each tag to be identified as being the specific mrna or not . a bayesian method has been used by audic and claverie ( 8) and vncio et al . comparison of this test and the test based on poisson - distributed tag counts used by madden et al . ( 19 ) shows a difference in sensitivity , the test of madden et al . being more conservative ( 10 ) . to quantify the transcripts of the two cell line , we have used the significance test ( ) of audic and claverie ( 8) and developed a tool to visualizing the analysis of differentially expressed tags in a scatter - plot shape ( log / log coordinates ) . websage is the software that enables a rapid and comprehensive analysis of the sage data and integrates statistical data analysis methods using a database system . websage enables simple and rapid analysis of a file of tags and the determination of differentially expressed tags ( p - values ) . to provide tag - to - gene links , three files from the ncbi ftp site ( sage genie ) were stored in database ( mysql ) and used at each analysis , to get the tag - to - gene assignment , gene accession number ( unigene and genbank ) and gene function information ( kegg , biocarta and gene ontology databank ) , respectively . sage genie provides the best match between gene name and tag , although there are options for manually viewing an alternate tag 's expression data . furthermore , the information is stored in a table and visualized in a scatter plot . figure 1 shows an example of websage analysis of our file of tags . the total number of tags analysed included 5257 tags from pancreatic cancer cell line and 6833 from cell line derived from liver metastasis . websage computed a p - value and classified the tags into three groups : green , p  0.01 ( significant ) ; red , p > 0.05 ( not significant ) ; and yellow , p in ] 0.01 ; 0.05 ] ( require further analysis ) . the information computed for the query is stored in a temporary table during the session . next , the data are exported in the csv format ( compatibility with all spreadsheets ) . our application is a web tool , developed in the php language using jpgraph library ( ) that has been modified . , websage is a useful web service that performs statistical analysis on the sage data , identifies the tags differentially expressed and shows the results in a scatter plot . moreover , the user can query with a specific tag and get a tag - to - gene assignment and gene function from kegg , biocarta and gene ontology databank . a total of 4809 tags from pancreatic cancer lines and an isogenic liver metastasis cell line were analysed . each plot corresponds to one tag or a set of tags , having the same p - value and the same apparition on the two libraries . . once clicked , a web page is displayed with a list of tags and their identification . the identification consists of the name of the tag and the corresponding gene , the p - value and the unigene and genbank accession number .
the serial analysis of gene expression ( sage ) is a powerful method to compare gene expression of mrna populations . to provide quantitative expression levels on a genome - wide scale , the cancer genome anatomy project ( cgap ) uses sage . 
 over 7 million sage tags , from 171 human cell types have been assembled . 
 the growing number of laboratories involved in sage research necessitates the use of software that provides statistical analysis of raw data , allowing the rapid visualization and interpretation of results . 
 we have created the first simple tool that performs statistical analysis on sage data , identifies the tags differentially expressed and shows the results in a scatter plot . 
 it is freely available and accessible at .