lysosomes are cellular organelles that play a pivotal role in the cell homeostasis through their involvement in degradation and recycling processes of extracellular material that has been internalized by endocytosis and intracellular components that have been sequestered by autophagy ( 1 ) . lysosomes may also fuse with the plasma membrane , emptying their contents outside the cell . this is important for processes such as cellular immune response and plasma membrane repair , both in normal and pathological conditions ( 2 , 3 ) . mutations that cause lysosomal enzyme deficiencies result in different syndromes , known as lysosomal storage disorders ( lsds ) ( 4 ) . in addition , they are characterized by intracellular deposition and protein aggregation , events also found in age - related neurodegenerative disorders , such as alzheimer s and parkinsons s diseases ( 57 ) . these studies underline the importance of the lysosome as a central player in cell metabolism . hence , the characterization of genes participating in lysosomal biogenesis and function is a critical step toward the understanding of basic processes in cell biology and pathogenic mechanisms in many human diseases . recently , it was found that most lysosomal genes exhibit coordinated transcriptional behavior and are regulated by the transcription factor eb ( tfeb ) , which also links autophagy to lysosome biogenesis ( 8 , 9 ) . gene expression at the post - transcriptional level can be regulated by micrornas ( mirnas ) . mirnas play important roles in diverse biological processes , including development , cell differentiation , proliferation and apoptosis , in which the lysosomal system also plays an important role . notably , mirnas have been recently identified as involved in the regulation of autophagy ( 10 , 11 ) . the human lysosome gene database ( hlgdb ) is the first searchable database focused on the census of genes belonging to the lysosomal system and on their regulation by mirnas . no database resources entirely dedicated to the regulation of lysosomal genes by mirnas or other regulators are currently available . several lists of lysosomal genes were collected from public gene databases , published proteomics articles and reviews edited by biochemists and cell biologists working in the lysosome field . we paid special attention on balancing predictions , which were as follows : ( i ) more suitable to look for confirmatory evidence ( targetscans ) ( 15 ) ; ( ii ) more suitable to identify any possible target for a particular mirna , to form the basis for in vitro or in vivo experiments ( pictar four - way and five - way ) ( 16 ) ; ( iii ) more suitable to find in silico evidence for the interaction between a mirna and a gene of a certain family or function ( pita , miranda ) ( 17 ) . to increase mirna - target mrna information , experimentally verified mirna targets from mirtarbase were also reported . hlgdb aims to providing a useful resource to anyone studying the lysosomes and a tool for identifying common regulatory features of lysosomal genes . hlgdb provides a user - friendly interface through which information can be easily retrieved , including the union and intersection of different gene lists , searches for mirna predictions and visualization on the gene transcript sequence of the mirna target predictions . the data reported in the current version 1.1 , derived from ncbi pubmed searches for review articles regarding human ( 8 , 1825 ) and murine ( 22 , 2629 ) proteome of the lysosome and from lists of lysosomal genes present in the gene ontology ( 30 ) , kegg ( 31 ) , reactome ( 32 ) and uniprot databases ( 33 ) [ uniprot :  lysosome ( kw-0458) and organism :  homo sapiens ( human ) ( 9606) ; kegg :  lysosome ( ko04142) ; go : go:0005764 data stamp from the source 20120303 ] . the references listed within each review article ( 34 , 35 ) were examined and lists of genes were extracted from either the full text or the supplementary information following a manual curation . there are 16 sources of information divided in four main categories : proteomic studies , databases , reviews and system biology approaches . each gene has been associated to its official hgnc gene symbol ( 36 ) and to its entrez gene i d ( mappings were based on data provided by entrez with a date stamp from the source of 7 march 2012 ) . the gene transcripts associated to each gene are annotated accordingly to ncbi refseq or genbank ( release 57 ) . mirna target predictions were extracted from the tables downloaded from the websites of the different algorithms used to predict the binding between mirna and gene transcripts . coordinated lysosomal expression and regulation ( clear ) is a nucleotide motif ( gtcacgtgac ) found to be highly enriched in the promoter set of lysosomal genes ( 8) . we mapped this motif on both strands on the human genome ( hg19 ) by means of fuzznuc utility of the emboss package allowing one single mismatch ( 37 ) . the binding sites of the tfeb come from a chip - seq experiment carried out on hela cell lines ( 38 ) . hlgdb is a mysql 5.0.95 database ( constructed in the fourth normal form , some redundancy being kept to increase retrieval performance ) , and the interface is built in php . search for gene lists : hlgdb can be used to retrieve and combine lists of lysosomal genes from different sources . the any and all options allow the user to either merge or intersect lists . search for a gene using the gene symbol is also allowed ( figure 1 ) . search for mirna targets : once the user has selected or created a gene list , he / she can find mirna ( or families of mirna ) targets choosing different combinations of prediction softwares . results are returned in a table showing information about the gene ( gene symbol and gene name ) and the mirna ( identifier and number of softwares predicting each binding ) . gene symbols are hyperlinked to gene - centered page where additional gene information is provided ( figure2a ) . on each gene transcript associated to the selected gene , search for tfeb binding sites / clear motifs : once the user has selected or created a list , he / she can choose the filter and find the lists of genes with tfeb binding sites or clear motifs within a range around the transcription start site ( tss ) . results are provided in the table showing gene - centered information , as the hit count and distance from tss of tfeb binding sites / clear motifs . all data in hlgdb are freely available for download as tab - delimited text files without password protection for any user . concerning other organisms , currently the database provides a parallel orthology annotation for mouse : the user can select the species of interest using the upper right botton . it collects information about these genes and their transcriptional regulation such as tfeb binding sites and mirnas . hlgdb was designed to become a lysosomal gene census . when new lysosomal genes will be discovered lysosomal genes include lysosomal hydrolases , lysosomal membrane proteins , lysosomal proteins involved in acidification and non - lysosomal proteins fundamental for this organelle biogenesis . in addition , there is increasing evidence that lysosome genes play a role in the pathogenesis of common neurodegenerative diseases such as alzheimer s , parkinson s and huntington s . researchers may benefit from hlgdb because they have in a single reference to the broadest compendium of lysosomal gene lists . results of mirna targets may be directly compared with other transcriptional regulation elements such as the distance from the tss of tfeb binding site or the distance to a clear sequence to identify common features of regulation . hlgdb has been designed to integrate additional layers of biological information , such as experimental data and comparative genomics . currently the database present information for human and mouse species ; in the next versions , additional species , such as rat , will be integrated . finally , hlgdb provides a powerful resource to system biology approaches and network analysis to dissect the map of interactions taking place in the lysosomal system .
lysosomes are cytoplasmic organelles present in almost all eukaryotic cells , which play a fundamental role in key aspects of cellular homeostasis such as membrane repair , autophagy , endocitosis and protein metabolism . 
 the characterization of the genes and enzymes constituting the lysosome represents a central issue to be addressed toward a better understanding of the biology of this organelle . in humans , mutations that cause lysosomal enzyme deficiencies result in > 50 different disorders and severe pathologies . 
 so far , many experimental efforts using different methodologies have been carried out to identity lysosomal genes . 
 the human lysosome gene database ( hlgdb ) is the first resource that provides a comprehensive and accessible census of the human genes belonging to the lysosomal system . 
 this database was developed by collecting and annotating gene lists from many different sources . 
 references to the studies that have identified each gene are provided together with cross databases gene related information . 
 special attention has been given to the regulation of the genes through micrornas and the transcription factor eb . 
 the hlgdb can be easily queried to retrieve , combine and analyze information on different lists of lysosomal genes and their regulation by microrna ( binding sites predicted by five different algorithms ) . 
 the hlgdb is an open access dynamic project that will permit in the future to collapse in a unique publicly accessible resource all the available biological information about lysosome genes and their regulation.database url : 
 http://lysosome.unipg.it/