at indiana universitypurdue university indianapolis ( iupui ) , we have developed a concept called  distributed drug discovery  ( d ) . herein is reported the first example of a key component of d : the distributed rehearsal of reagents and their subsequent use in real and virtual catalog production . the premise of d is that the availability of simple , inexpensive equipment and procedures for each of the core scientific drug discovery disciplines ( computational chemistry , synthetic chemistry , and biochemical screening ) will enable large drug discovery problems to be broken down into manageable smaller units to be solved at multiple sites throughout the developing and developed world . the recombined and coordinated results of these resources can inexpensively accelerate the identification of leads ( primarily for developing world and other neglected disease targets ) in the early stages of the drug discovery process . in addition , this effort provides educational and job opportunities in both the developed and developing worlds while building cultural and economic bridges for the common good . combinatorial virtual catalogs , realistically accessible by globally distributed synthesis , are key to the implementation of d. in classical combinatorial chemistry , reagent rehearsal increases the likelihood that any individual member of an enumerated virtual library can be synthesized from each unique combination of rehearsed reagents . however , it imposes additional strong constraints on reagent - rehearsed virtual catalogs : any member of the library must be accessible by rehearsed or precedented synthesis , anywhere in the world , using simple , inexpensive equipment and procedures . part i reports the first successful global reagent rehearsal , conducted by undergraduate and graduate students in the united states , poland , russia , and spain . in part ii , a virtual d catalog based on this work one of the most common types of intermediates used in combinatorial chemistry is the resin - bound natural or unnatural -amino acid 1 ( scheme 1 ) . the -amino acid substructure occurs in 28% of the structures cited in a recent  comprehensive survey of combinatorial library synthesis  . scheme 1 gives examples of a few of the published libraries based on 1 . with the exception of proline , the methodology for the introduction of side chains by carboncarbon bond construction has expanded considerably the availability of these unnatural -amino acids . of the existing routes to such molecules thus , introduction of an unnatural side chain onto the -position of a suitable glycine or higher amino acid derivative by alkylation can provide , respectively , -substituted or ,-disubstituted amino acid products . because of the central role of 1 in combinatorial chemistry , and the ability to greatly expand its numbers through alkylation chemistry , we chose to focus on rehearsing alkylating agents r1x for our first exemplification of d synthesis . in the process , we wished to show the likelihood that undergraduate or graduate students would be able to make any given member of a resulting virtual d catalog , further demonstrating the potential of distributed drug discovery . we modified our published synthesis of 1(17c ) and incorporated the rehearsal into a student combinatorial chemistry laboratory that transformed 1 to the acylated derivatives 5 ( scheme 2 ) . this generic structure 5 would then become the basis of our first virtual d catalog . the time ranges shown in scheme 2 accommodated the details of laboratory scheduling in academic institutions at different geographic locations . simple , inexpensive  bill - board 6-pack  equipment ( figure 1 ) was used to carry out this procedure , six reactions at a time . every bill - board 6-pack had six reaction vessels in a grid of two rows ( a and b ) by three columns ( 1 , 2 , and 3 ) , and reactions were correspondingly identified by  row , column  ( e.g. , a1b3 ) . the equipment was designed to enable the essential steps common to most solid - phase synthetic procedures : cycles of reaction and filtration workup , followed by product cleavage , and collection . bill - board components and 2  3 experimental grid . in this first demonstration of the distributed process , twenty - four alkylating agents r1x were rehearsed , often in quadruplicate , sometimes at geographical locations remote from one another ( indianapolis , in ; lublin , poland ; moscow , russia ; and barcelona , spain ) . in all cases the quality of the crude products was assessed by lc / ms to obtain a marker of the suitability of a given reagent in combinatorial library generation . this analytical measurement of quality is not definitive because uv detection can overlook , among other things , poor absolute recovery , the presence of insoluble impurities , or soluble impurities not revealed by the detection system . nevertheless , the lc / ms data permits elimination , from a rehearsed virtual library , products based on alkylating agents that are clearly problematic . follow up synthesis and purification conducted at iupui allowed the isolation and characterization of all the unnatural amino acids reported in this distributed reagent rehearsal study . since reagent rehearsal was done by students with little to no prior experience in either organic synthesis or solid - phase chemistry , two key elements were incorporated into the laboratory : ( 1 ) students worldwide conducted the same  control  alkylation reaction , using r1x = benzyl bromide ( 6 ) in column 1 . ( 2 ) the evaluation of new alkylating agents was replicated by at least one other student or student team . this worldwide distributed process permits the assessment of both the quality of the work carried out by any given student / team and the reproducible efficacy of the evaluated reagents . the first synthetic rehearsals were conducted at iupui , utilizing four concurrent laboratory sections , to pilot the process of replicated synthesis in multiple students hands . there were up to 20 students in each section . a total of 65 students operated in 32 teams of two per bill - board ( 192 reactions ) , with one extra bill - board used for the solo student . the control alkylating agent , benzyl bromide ( 6 ) , was used by all students in column 1 ( figure 1 ) . the new alkylating agents were rehearsed in columns 2 and 3 . in these experiments , the acylating agents used in rows a and b of the bill - board were always fmoc chloride and 2-naphthoyl chloride , respectively . in this first extensive study , two commercial suppliers , i and ii , were commissioned to produce the starting benzophenone imine resin 2 . for comparison purposes , resin from source i was used in sections one , two , and four ( teams 18 , 1017 , and 2633 ) , and resin from source ii was used in section three ( teams 1825 ) . since all 32 teams prepared the same product ( fmocphe 5{6}a , see table 1 ) in position a1 of their bill - boards it was informative to analyze the quality , as a function of resin source , of all 32 of these replicated products . figure 2 shows a comparison of representative products a1 from teams using resins from these two suppliers . representative uv trace from lc / ms analysis of a1 from either resin supplier i or ii . ( a ) lc trace of product ( team 1 , al ) using resin from supplier i. ( b ) lc trace of product ( team 18 , al ) using resin from supplier ii . in the products obtained from reactions using resin from i ( figure 2a ) , there was , in addition to desired product ( 5.04 min , m + 1 = 388 , 77% ) , the universal presence of three impurities ( 2.5 min , m + 1 = 256 , 4% ; 4.8 min , m + 1 = 445 , 11% ; 5.3 min , m + 1 = 494 , 8% ) . the ms data allowed the tentative assignment of the structures 23 , 24 , and 25 for these respective peaks ( figure 3 ) . in contrast , there were insignificant levels of the impurities at 2.5 and 4.8 min in samples prepared from resin supplier ii ( figure 2b ) . the supporting information contains a more complete discussion of the origin of these proposed impurities 23 and 24 in resin from i , and 25 in both resins from either i or ii . proposed structures for impurities produced from resin source i. although resins from i and ii were used in the initial laboratories at iupui , because of its higher quality , only resin from commercial source ii was used in subsequent laboratories at iupui , and in laboratories conducted in poland , russia , and spain . replicated alkylations ( 24 replicates ) , using the same resin and alkylating agent , gave strikingly similar results ( table 1 ) . individual results rarely differed from the average by more than 3% . as expected , because of the impurities present in the starting resin from i ( see earlier discussion and supporting information ) the purity of alkylation products from this resin was lower ( by 1520% ) than those from ii ( compare , for example , the results for the fmoc derivatives 5{7}a through 5{12}a from alkylating agents 7 through 12 , where the first two results for each compound were from resin source i , and the second two results were from resin source ii ) . nevertheless , from the same resin source the alkylation results are very reproducible . in rare cases , this is to be expected when so many different researchers ( students ) are performing this work for the first time . for example , team 28 obtained none of the expected products 5{15}b or 5{17}b , whereas three other teams using the same reagent combinations ( teams 12 , 13 , 29 and 11 , 12 , 27 ) were successful and had similar results ( 63%  3% and 80%  8% , respectively ) for each of these compounds in the appropriate bill - board positions . encouraged by the success of the iupui student alkylating agent rehearsal , we tested the ability to further distribute this process to students outside of the united states . using the same procedures ( with minor modifications ) , equipment , and resin from source ii , the laboratory was first replicated in spain . in 36 separate reactions , teams at the university of barcelona ( two or three students per bill - board ) evaluated control benzyl bromide ( 6 , six replicates ) and six additional alkylating agents in duplicate ( table 2 ) . with the exclusive use of starting resin purchased from supplier ii , the barcelona laboratories obtained excellent results , providing strong documentation for the ability of distributed discovery to reproducibly synthesize molecules , both within the same site , and around the world . barcelona , team 3 ) , all the control fmocphe samples 5{6}a were synthesized in > 92% purity as determined by lc / ms ( table 2 ) . this was consistent with the data generated in the united states at iupui , where the subset of compounds 5{6}a synthesized used starting resin from the same supplier ( ii ) produced 7 of 8 products in > 93% purity ( table 1 ) . the barcelona laboratories also rehearsed , in duplicate , six new alkylating agents , 2631 . the data from teams 2 and 3 for 5{28}b indicates that the alkylating agent 28 is also likely to give desired product but with moderate purity . the methoxy - substituted reagents 29 and 31 illustrate the need for a nuanced interpretation of results . the crude material recoveries for the methoxy derivatives 5{29}a , b and 5{31}a , b were notably lower compared to the rest of the products in this laboratory . these molecules , as anisole derivatives , are susceptible to friedelcrafts reactions . it is well established in the peptide field that , in the absence of a powerful cation quenching agent ( such as anisole itself ) , products capable of friedelcrafts reactions can reattach themselves to the resin cation generated in the cleavage process . accordingly , selective , cleavage - condition dependent removal of desired product ( by reattachment to the resin ) could mask a successful alkylation prior to cleavage . to further demonstrate the ability of the distributed reagent rehearsal project to provide replicated results in student laboratories across the globe , table 3 compares a set of molecules prepared at iupui ( united states ) , the university of barcelona ( spain ) , moscow state university ( russia ) , and the lublin school of pharmacy ( poland ) . these results indicate that d syntheses can be carried out at multiple global locations and afford reproducible results . the product 5{32}a ( figure 4 ) was obtained in 76% purity ( lc / ms analysis ) . moscow single - site result . to fully characterize products from the reagent rehearsal and confirm that student - generated results can reliably predict synthetic outcomes , all of the listed alkylating agents were subsequently re - evaluated by a postdoctoral fellow at iupui . using resin from source ii , the experimental procedure used was identical to that employed in the rehearsal laboratories at iupui . the results , summarized in table 4 , confirm the ability of student rehearsed chemistry to be replicated by a third party . these results also resolved the questions arising from the large variability of results in the rehearsal of reagents 28 and 29 reported in table 2 . in addition , the single result reported by the moscow site for reagent 32 was confirmed . the supporting information provides lc / ms data for all the crude products from indianapolis , lublin , and moscow , along with nmr data for all the purified , new compounds prepared . rl = averaged results from student rehearsal laboratories ; pd = result from postdoc synthesis at iupui . single value , other value was 0% . in summary , these first reagent rehearsal laboratories document a key requirement of the distributed drug discovery concept : the global ability of students to evaluate reagents and reproducibly make new potential drug leads , using simple , inexpensive equipment and procedures . these reagents are now acceptable as input in the enumeration of the d accessible virtual catalogs to be described in part ii . an essential component of distributed drug discovery is the open - access availability of databases of molecules accessible through distributed chemistry . computational chemists can then analyze these large databases to identify and make globally available smaller databases of potential drug leads targeted for developing world diseases . subsequently , the synthesis of the selected molecules can be carried out , in a distributed fashion , at sites across the world . the compounds ( including stereoisomers when appropriate ) in this virtual d catalog were enumerated with commercial software . to ensure that virtual library generation is not limited by cost we also enumerate libraries with alternative software currently available for free to academia . on the basis of the distributed chemistry developed and reported in this article , two types of library enumeration were envisioned . , a much larger ,  virtual  d catalog ( including the compounds actually made ) can be enumerated from all combinations of rehearsed or otherwise well - precedented reagents used at the variable alkylation and acylation steps . to illustrate this process , we assembled lists of acceptable , commercially available , alkylating and acylating agents ( tables 5 and 6 , respectively ) to enumerate a representative virtual d catalog of acylated unnatural amino acids ( scheme 2 ) . for the alkylation step extensive precedence is available , both from our solution- and solid - phase studies and those of others , to predict the reactivity of schiff base esters and related systems with these electrophiles under a variety of basic conditions . for example , even though michael addition reactions were not rehearsed in the present study , we have confidence , based on our earlier solid - phase research using resin - bound schiff bases and michael acceptors as electrophiles , that reactants 93108 ( table 5 ) can be utilized successfully . , we plan to incorporate all rehearsed reagent information into a single database to further assist reagent selection . in the acylation step however , we have since modified our d laboratories to use carboxylic acids to form the amides by traditional carbodiimide mediated couplings . this makes available a much wider repertoire of inexpensive reactants for enumeration at the second variable position . because the reactivity of carboxylic acids in forming amide bonds is extensively documented , simple chemical intuition and reactivity considerations were used to select the 100 carboxylic acids ( table 6 ) for enumeration . on the basis of these considerations , a virtual d catalog of 24  416 acylated unnatural amino acids 5 was enumerated . as discussed in the following article , an additional 24  192 methyl esters of these acylated unnatural amino acids were enumerated on the basis of the cleavage of resin - bound products 4 by methanolysis . thus , a total of 48  608 unique products are available for d catalog access and searching . the 100 commercially available alkylating agents and michael acceptors and 100 carboxylic acids used to perform these enumerations are shown in tables 5 and 6 , respectively . the catalog of acylated unnatural amino acids contains more than the 20  000 members that would arise from a simple 100  100 combinatorial process . this is caused by the formation of stereoisomers in the alkylation step and their combination with other stereoisomers sometimes present in the alkylating or acylating reagents . thus , from two to eight stereoisomers appear for each structure in the virtual d catalog . the separate listing of each of these anticipated stereoisomeric products allows appropriate computational discrimination and selection when three - dimensional modeling software is employed and chirality is important . two stereoisomers arise when there are no other stereogenic elements in either the electrophile or the acid [ e.g. , ( r)-5{12,207 } plus enantiomer ] or when the electrophile or acid is present as a single enantiomer ( r , r)-5{22,117 } plus diastereomer ] or geometric isomer ( r , e)-5{82,208 } plus enantiomer ] . four stereoisomers are contained in the library when a racemic or prochiral electrophile or a racemic carboxylic acid is used ( s , s)-5{18,121 } or ( r)-5{93,159 } plus three other stereoisomers for each ] . eight stereoisomers arise when both a racemic electrophile and a racemic carboxylic acid are used ( r , r , r)-5{47,186 } plus seven other stereoisomers ] . the synthesis of stereoisomeric mixtures of unnatural amino acid derivatives has the advantage of providing stereoisomers for evaluation . the follow up work to identify the active stereoisomer can be accomplished , without stereoselective synthesis , through bioassay guided fractionation or chiral chromatography . the active stereoisomer identified by these techniques can be subsequently prepared by known stereoselective synthetic routes , separation techniques , or resolution procedures . it is important to consider potential side - reactions during the various steps of the synthesis of acylated unnatural amino acids 5 . for example , the tert - butyl esters present in resin - bound products 4 that originated in the reactants used for alkylation , michael addition , or acylation ( 36 , 75 , 96 ) will be converted to the carboxylic acids during tfa cleavage . likewise , the t - butyl carbamate ( n - boc ) group in reactant 83 will be cleaved to the free indole nh on tfa cleavage to products 5 . a lead reference for each of these subsequent reactions is noted with the respective reactant , and this is reflected in the enumerated product structures . other more subtle side reactions will be incorporated into the enumeration products as they are discovered on the basis of literature precedence or rehearsal of reagents by our group or by others . to illustrate the variety of virtual molecules available through this enumeration the open - access availability of this virtual d catalog makes possible the global computational selection of candidates for drug synthesis , while simultaneously enabling the prioritization of future reagent rehearsals . when computational analysis suggests a particular library member may be a drug lead for a developing world disease target , that molecule will be selected for distributed synthesis . if the reagent used as input in its enumeration is unrehearsed , the synthesis will evaluate its suitability as a reagent and enable it to become a part of this expanding rehearsed reagent database , stored electronically and available through open - access . students carry out , using simple procedures and inexpensive equipment , worldwide replicated solid - phase combinatorial chemistry , rehearsing new reagents and generating new compounds for library production . at the same time they learn and develop important synthetic skills and methodologies . the data generated and reported here allows the construction of a globally accessible d database of well - documented alkylating agents and michael acceptors for the synthesis of resin - bound unnatural amino acids 1 . this intermediate can then be incorporated into a wide variety of published combinatorial chemistry procedures . virtual d catalogs based on rehearsed or well - precedented reagents and inexpensive global procedures are essential to the goals of distributed drug discovery . accordingly , we have enumerated a 24  416-member virtual d catalog of acylated unnatural amino acids 5 based on the current work . freely available virtual d catalogs , such as the one reported in this article , can be computationally analyzed to select potential drug leads for developing - world and other neglected diseases . in turn , the distributed reagent rehearsal provides strong precedent and documented procedures to successfully make , through distributed synthesis , any of the molecules selected by this process . anhydrous nmp , chloroform - d1 , 2-fluorobenzyl bromide , 4-fluorobenzyl bromide , 2-(trifluoromethyl)benzyl bromide , 4-(trifluoromethyl)benzyl bromide , 2-bromobenzyl bromide , allyl bromide , 2-nitrobenzyl bromide , 2,4-dichlorobenzyl chloride , and benzyl 2-bromoacetate and were purchased from acros organics . btpp ( tert - butyl - imino - tri(pyrrolidino)phosphorane ) . and fmoc chloride were purchased from fluka . 5-(chloromethyl)-2-chloropyridine , n , n - diisopropylethylamine , benzyl bromide , 3-fluorobenzyl chloride , 3-(trifluoromethyl)benzyl chloride , 3-bromobenzyl bromide , 4-bromobenzyl bromide , 2-(bromomethyl)benzonitrile , 3-(bromomethyl)benzonitrile , 4-(bromomethyl)benzonitrile , 2-iodobenzyl bromide , 4-iodobenzyl bromide , 1-(chloromethyl)naphthalene , 2-(bromomethyl)naphthalene , 4-methoxybenzyl bromide , 3-methoxybenzyl bromide , and methanol - d4 were obtained from aldrich chemical co. all resins were purchased from midwest bio - tech laboratories ( resin i ) or polymer laboratories ( resin ii ) unless otherwise noted . manual solid - phase organic syntheses at ambient temperature were carried out in 3.5 ml fritted glass reaction vessels equipped with polypropylene screw caps with teflon - faced silicon septa on the bill - board set , which was designed by one of us ( wls ) as inexpensive equipment to simplify and expedite multiple , manual solid - phase syntheses . the bill - board reaction vessel components are available from chemglass : iup-0305270h for 3.5 ml reaction vessel ; iup-0305280h ( polypropylene screw cap ) ; iup-0305281h ( teflon faced silicone septa ) . analytical thin layer chromatography ( tlc ) flash column chromatography was performed on silica gel 60 ( 230400 mesh ) from silicycle chemical division . the yields of the final compounds , after chromatographic purification , were calculated on the basis of the initial loading of the starting resins and are the overall yields of all reaction steps starting from these resins . h nmr spectra were recorded at 200 mhz ( varian spectrometer ) using tms ( 0.00 ppm ) and chloroform - d1 mixed with methanol - d4 ( 310% ) . electrospray ionization mass spectrometry was conducted using a pesciex api iii triple stage quadrupole mass spectrometer operated in either positive - ion or negative - ion detection mode . the composition of reaction mixtures was determined based on the integration of nmr spectra as well as lc - ms results . lc - ms analyses were conducted using an agilent system , consisting of a 1100 series hplc connected to a diode array detector and a 1946d mass spectrometer configured for positive - ion / negative - ion electrospray ionization . the lc - ms samples were analyzed as solutions in ch3cn , prepared at 0.080.12 mg / ml concentration . the lc - ms derived composition of mixtures was determined based on uv integration at 210 nm . depending on the number of reactions to be performed , resin 2 was distributed either by weight or as aliquots from an isopycnic suspension . in the case of distribution by volume from an isopycnic suspension , the bill - boards were placed in their drain trays , and from a neutral buoyancy suspension in ch2cl2/nmp , 50 mols of the benzophenone imine of glycine wang resin ( 2 , the resin loading is 0.7  0.9 mmol / g ) was distributed , via repeated 1 ml aliquots , to each of the six reaction vessels in a given bill - board 6-pack . during the distribution of the resin , when distribution was complete , residual solvent was removed with an  air - push  from a disposable plastic pipet ( fisher , 1371123 ) fitted with a pierced septum ( aldrich , z 127434 ) . the resin was washed with nmp ( 3  3 ml ) ( this nmp wash was also done when resin was weighed out into reaction vessels ) . the bottom of each reaction vessel was then capped , and a new calibrated pipet ( fisher , 13 - 711 - 24 ) was used for adding each alkylation reagent in the following step . the first r1-x ( benzyl bromide ) [ 0.5 ml of a 0.20 m solution in nmp , 100 mols , 2 equiv ] was added to the resin in the two reaction vessels in the first column positions ( i.e. , a1 and b1 ) . then the second r1-x [ 0.5 ml of a 0.20 m solution in nmp , 100 mols , 2 equiv ] was added to the resin in the two vessels in the second column positions ( i.e. , a2 and b2 ) . finally , the third r1-x [ 0.5 ml of a 0.20 m solution in nmp , 100 mols , 2 equiv ] was added to the resin in the two vessels in the third column positions ( i.e. , a3 and b3 ) . base was then added [ 0.5 ml of a 0.20 m solution of btpp in nmp , 100 mols , 2 equiv ] to each of the six reaction vessels . the tops of all reaction vessels were capped , and the bill - board was placed in a rotation apparatus . depending on the school location and laboratory schedule , the reaction was allowed to proceed from 24 h to 2 days with rotation . the bill - board was removed from the rotation apparatus and , after inverting the board , the bottom caps were removed . the board was then placed , top side up , in the drain tray , the top caps were removed , and the reagents from the alkylation step were allowed to drain , followed by an air - push . the alkylated resin - bound resin products 3 were washed with thf ( 1  3 ml ) . using 6 clean caps , the bottom of each reaction vessel was capped , and a 1 n aqueous hcl / thf solution ( 1:2 , 2.5 ml ) was added to each of the six reaction vessels . the caps were then put on the top of each vessel , and the bill - board was returned to the rotator for 20 min . the reagents and byproduct from the hydrolysis were then removed from hydrolyzed resin - bound product ( 1 ) by filtration and washing with thf ( 1  3 ml ) and then nmp ( 1  3 ml ) . the bottom of each reaction vessel was capped , and the acylating agent , r2cocl [ 0.5 ml of a 0.30 m solution of r2cocl in nmp , 150 mols , 3 equiv ] was added to the resin 1 in each of the three vessels in row a ( a1 , a2 , and a3 ) . then the second acylating agent , r2cocl [ 0.5 ml of a 0.30 m solution of r2cocl in nmp , 150 mols , 3 equiv ] was added to the resin 1 in each of the three vessels in row b ( b1 , b2 , and b3 ) . this was followed by addition of diea [ 0.5 ml of a 0.30 m solution in nmp , 150 mols , 3 equiv ] to each of the six reaction vessels . the tops of the reaction vessels were capped , and the reaction was allowed to rotate overnight or up to 5 days ( again , dependent on school laboratory schedules ) . acylated resin product 4 was washed with nmp ( 2  3 ml ) , thf ( 2  3 ml ) , and ch2cl2 ( 3  3 ml ) . the product was cleaved from resin with tfa / h2o ( 95:5 , 2 ml ) for 30 min . the filtrate from the cleavage reaction was collected , and the resin was washed with tfa / h2o ( 95:5 , 2 ml ) and ch2cl2 ( 1  2 ml ) . after each collection vial was swirled to thoroughly mix the cleavage and rinse solutions , a sample of each solution ( 100 l ) containing product 5 was transferred to an autosampler vial for lc / ms analysis . designed to speed up the evaporation with a stream of nitrogen , in a contained system , while trapping the evaporating tfa in 2 n naoh . purification of the crude product was performed via flash column chromatography , using the glass fritted reaction vessel ( 3.5 ml ) loaded with bulk silica gel ( 650700 mg ) . the crude product was dissolved in ch2cl2 and , if necessary , meoh ( total solution volume 0.5 ml ) and applied to the column . then a gradient of eluting solvent was applied starting with ch2cl2 to ch2cl2/meoh / h2o ( 300:10:0.5 ) . the purified product was dried in the evaporation equipment described above at ambient temperature under a flow of nitrogen . forty eight hours later ( wednesday ) , the reactions were worked - up ; the imine was hydrolyzed and worked - up , and the acylation reactions were begun . five days later ( the following monday ) , the acylation reactions were worked - up ; the product was cleaved from the resin , and samples taken for tlc and lc / ms analysis . all samples were analyzed by lc / ms in the analytical department at eli lilly & company ( indianapolis ) . at each of these locations , the next day the reactions were worked - up ; the imine was hydrolyzed and worked - up , and the acylation reactions were begun . the next ( and last ) day the acylation reactions were worked - up ; the product was cleaved from the resin , and samples taken for tlc and lc / ms analysis . the samples from moscow and lublin were taken back to indianapolis where they were analyzed by lc / ms in the analytical department at eli lilly . 6.9 mg ( 35% isolated yield ) following chromatographic purification ( ch2cl2/meoh / h2o , 300:10:0.5 ) ; initial lc / ms purity 82% , tr = 5.11 min ; h nmr ( 200 mhz , cdcl3/cd3od )  3.003.14 ( m , 1h ) , 3.20 ( dd , j = 13.9 hz , j = 5.5 hz , 1h ) , 4.144.27 ( m , 1h ) , 4.284.49 ( m , 2h ) , 4.59 ( t , j = 5.8 hz , 1h ) , 7.16 ( m , 2h ) , 7.237.46 ( m , 7h ) , 7.527.62 ( m , 2h ) , 7.78 ( d , j = 6.8 hz , 2h ) ; lc / ms calcd for c24h22no4 [ m + h ] 388.1 ; found 388.1 . 8.3 mg ( 41% isolated yield ) following chromatographic purification ( ch2cl2/meoh / h2o , 300:10:0.5 ) ; initial lc / ms purity 83% , tr = 5.12 min;h nmr ( 200 mhz , cdcl3/cd3od )  3.09 ( dd , j = 14.2 hz , j = 7.7 hz , 1h ) , 3.32 ( dd , j = 14.2 hz , j = 4.9 hz , 1h ) , 4.124.23 ( m , 1h ) , 4.244.44 ( m , 2h ) , 4.61 ( t , j = 5.5 hz , 1h ) , 6.967.10 ( m , 2h ) , 7.147.45 ( m , 6h ) , 7.517.60 ( m , 2h ) , 7.76 ( d , j = 7.2 hz , 2h ) ; lc / ms calcd for c24h21fno4 [ m + h ] 406.1 ; found 406.2 . 10.0 mg ( 49% isolated yield ) following chromatographic purification ( ch2cl2/meoh / h2o , 300:10:0.5 ) ; initial lc / ms purity 82% , tr = 5.17 min ; h nmr ( 200 mhz , cdcl3/cd3od )  3.08 ( dd , j = 13.8 hz , j = 6.2 hz , 1h ) , 3.19 ( dd , j = 13.9 hz , j = 5.5 hz , 1h ) , 4.20 ( t , j = 7.0 hz , 1h ) , 4.284.51 ( m , 2h ) , 4.60 ( t , j = 5.5 hz , 1h ) , 6.857.00 ( m , 2h ) , 7.177.45 ( m , 6h ) , 7.517.63 ( m , 2h ) , 7.77 ( d , j = 7.4 hz , 2h ) ; lc / ms calcd for c24h21fno4 [ m + h ] 406.1 ; found 406.3 . 7.0 mg ( 34% isolated yield ) following chromatographic purification ( ch2cl2/meoh / h2o , 300:10:0.5 ) ; initial lc / ms purity 97% , tr = 5.18 min ; h nmr ( 200 mhz , cdcl3/cd3od )  3.02 ( dd , j = 13.8 hz , j = 6.0 hz , 1h ) , 3.15 ( dd , j = 14.1 hz , j = 5.3 hz , 1h ) , 4.19 ( t , j = 6.7 hz , 1h ) , 4.284.52 ( m , 2h ) , 4.56 ( m , 1h ) , 6.95 ( m , 2h ) , 7.057.15 ( m , 2h ) , 7.287.46 ( m , 4h ) , 7.527.61 ( m , 2h ) , 7.77 ( d , j = 7.2 hz , 2h ) ; lc / ms calcd for c24h21fno4 [ m + h ] 406.1 ; found 405.8 . 12.2 mg ( 54% isolated yield ) following chromatographic purification ( ch2cl2/meoh / h2o , 300:10:0.5 ) ; initial lc / ms purity 94% , tr = 5.46 min ; h nmr ( 200 mhz , cdcl3/cd3od )  3.10 ( dd , j = 14.2 hz , j = 9.6 hz , 1h ) , 3.46 ( dd , j = 14.7 hz , j = 5.1 hz , 1h ) , 4.074.20 ( m , 1h ) , 4.214.36 ( m , 2h ) , 4.64 ( dd , j = 9.5 hz , j = 5.1 hz , 1h ) , 7.247.48 ( m , 7h ) , 7.54 ( t , j = 6.6 hz , 2h ) , 7.64 ( d , j = 7.4 hz , 1h ) , 7.75 ( d , j = 7.4 hz , 2h ) ; lc / ms calcd for c25h19f3no4 [ m + h ] 456.1 ; found 456.2 . 9.4 mg ( 41% isolated yield ) following chromatographic purification ( ch2cl2/meoh / h2o , 300:10:0.5 ) ; initial lc / ms purity 90% , tr = 5.53 min ; h nmr ( 200 mhz , cdcl3/cd3od )  3.14 ( dd , j = 13.8 hz , j = 6.0 hz , 1h ) , 3.26 ( dd , j = 13.7 hz , j = 5.3 hz , 1h ) , 4.19 ( t , j = 6.6 hz , 1h ) , 4.274.51 ( m , 2h ) , 4.61 ( t , j = 5.5 hz ) , 7.287.50 ( m , 8h ) , 7.517.62 ( m , 2h ) , 7.77 ( dd , j = 7.4 hz , 2h ) ; lc / ms calcd for c25h19f3no4 [ m + h ] 456.1 ; found 456.1 . 9.4 mg ( 41% isolated yield ) following chromatographic purification ( ch2cl2/meoh / h2o , 300:10:0.5 ) ; initial lc / ms purity 88% , tr = 5.55 min ; h nmr ( 200 mhz , cdcl3/cd3od )  3.12 ( dd , j = 13.7 hz , j = 5.9 hz , 1h ) , 3.26 ( dd , j = 14 hz , j = 5.6 hz , 1h ) , 4.19 ( t , j = 6.6 hz , 1h ) , 4.304.54 ( m , 2h ) , 4.63 ( t , j = 5.3 hz , 1h ) , 7.217.46 ( m , 6h ) , 7.477.61 ( m , 4h ) , 7.77 ( d , j = 7.2 hz , 2h ) ; lc / ms calcd for c25h19f3no4 [ m  h ] 454.1 ; found 454.2 . 11.2 mg ( 48% isolated yield ) following chromatographic purification ( ch2cl2/meoh / h2o , 300:10:0.5 ) : initial lc / ms purity 90% , tr = 5.39 min ; h nmr ( 200 mhz , cdcl3/cd3od )  3.12 ( dd , j = 13.8 hz , 9.2 hz , 1h ) , 3.44 ( dd , j = 14.1 hz , 5.3 hz , 1h ) , 4.054.40 ( m , 3h ) , 4.68 ( dd , j = 8.8 hz , 5.1 hz , 1h ) , 7.037.16 ( m , 1h ) , 7.187.45 ( m , 6h ) , 7.55 ( d , j = 7.6 hz , 3h ) , 7.76 ( d , j = 7.4 hz , 2h ) ; lc / ms calcd for c24h21brno4 [ m + h ] 466.1 ; found 468.0 . 10.7 mg ( 46% isolated yield ) following chromatographic purification ( ch2cl / meoh / h2o , 300:10:0.5 ) ; initial lc / ms purity 97% , tr = 5.49 min ; h nmr ( 200 mhz , cdcl3/cd3od )  3.05 ( dd , j = 13.8 hz , 6.6 hz , 1h ) , 3.17 ( dd , j = 13.9 hz , 5.5 hz , 1h ) , 4.154.51 ( m , 3h ) , 4.59 ( t , j = 5.5 hz , 1h ) , 7.067.21 ( m , 2h ) , 7.307.46 ( m , 6h ) , 7.527.63 ( m , 2h ) , 7.77 ( d , j = 7.4 hz , 2h ) ; lc / ms calcd for c24h21brno4 [ m + h ] 466.1 ; found 466.1 . 9.8 mg ( 42% isolated yield ) following chromatographic purification ( ch2cl2/meoh / h2o , 300:10:0.5 ) ; initial lc / ms purity 98% , tr = 5.52 min ; h nmr ( 200 mhz , cdcl3/cd3od ) :  3.03 ( dd , j = 13.6 hz , j = 6.1 hz , 1h ) , 3.15 ( dd , j = 14.4 hz , j = 5.8 hz , 1h ) , 4.19 ( t , j = 6.2 hz , 1h ) , 4.284.51 ( m , 2h ) , 4.524.62 ( m , 1h ) , 7.01 ( d , j = 8.4 hz , 2h ) , 7.317.46 ( m , 6h ) , 7.537.62 ( m , 2h ) , 7.78 ( d , j = 7.0 hz , 2h ) ; lc / ms calcd for c24h21brno4 [ m + h ] 466.1 ; found 467.8 . 7.8 mg ( 38% isolated yield ) following chromatographic purification ( ch2cl2/meoh / h2o , 300:10:0.5 ) ; initial lc / ms purity 94% , tr = 4.87 min ; h nmr ( 200 mhz , cdcl3/cd3od ) :  3.25 ( dd , j = 14.3 hz , j = 7.7 hz , 1h ) , 3.48 ( dd , j = 14.3 hz , j = 5.5 hz , 1h ) , 4.16 ( t , j = 6.7 hz , 1h ) , 4.224.44 ( m , 2h ) , 4.604.76 ( m , 1h ) , 7.267.64 ( m , 10h ) , 7.76 ( d , j = 7.0 hz , 2h ) ; lc / ms calcd for c25h19n2o4 [ m + h ] 413.1 ; found 413.2 . 7.8 mg ( 38% isolated yield ) following chromatographic purification ( ch2cl2/meoh / h2o , 300:10:0.5 ) ; initial lc / ms purity 82% , tr = 4.81 min;h nmr ( 200 mhz , cdcl3/cd3od )  3.09 ( dd , j = 13.8 hz , j = 6.2 hz , 1h ) , 3.22 ( dd , j = 13.5 hz , j = 5.1 hz , 1h ) , 4.19 ( t , j = 6.4 hz , 1h ) , 4.294.54 ( m , 2h ) , 4.58 ( m , 1h ) , 7.277.62 ( m , 10h ) , 7.77 ( d , j = 7.4 hz , 2h ) ; lc / ms calcd for c25h19n2o4 [ m  h ] 412.1 ; found 411.3 . 7.5 mg ( 36% isolated yield ) following chromatographic purification ( ch2cl2/meoh / h2o , 300:10:0.5 ) ; initial lc / ms purity 91% , tr = 4.79 min ; h nmr ( 200 mhz , cdcl3/cd3od )  3.10 ( dd , j = 13.7 hz , j = 7.2 hz , 1h ) , 3.193.33 ( m , 1h ) , 4.19 ( t , j = 6.4 hz , 1h ) , 4.314.55 ( m , 2h ) , 4.564.64 ( m , 1h ) , 7.227.46 ( m , 6h ) , 7.527.62 ( m , 4h ) , 7.78 ( d , j = 7.4 hz , 2h ) ; lc / ms calcd for c25h19n2o4 [ m  h ] 412.1 ; found 412.5 . 11.4 mg ( 44% isolated yield ) following chromatographic purification ( ch2cl2-meoh - h2o , 300:10:0.5 ) ; initial lc / ms purity 97% , tr = 5.51 min ; h nmr ( 200 mhz , cdcl3/cd3od )  3.11 ( dd , j = 14.4 hz , j = 9.2 hz , 1h ) , 3.40 ( dd , j = 13.9 hz , j = 5.1 hz , 1h ) , 4.094.21 ( m , 1h ) , 4.224.39 ( m , 2h ) , 4.67 ( dd , j = 9.2 hz , j = 5.1 hz , 1h ) , 6.866.97 ( m , 1h ) , 7.227.44 ( m , 6h ) , 7.527.61 ( m , 2h ) , 7.75 ( d , j = 7.4 hz , 2h ) , 7.83 ( d , j = 7.8 hz , 1h ) ; lc / ms : calcd for c24h21ino4 [ m + h ] 514.0 ; found 514.0 . 7.1 mg ( 28% isolated yield ) following chromatographic purification ( ch2cl2/meoh / h2o , 300:10:0.5 ) ; initial lc / ms purity 97% , tr = 5.61 min ; h nmr ( 200 mhz , cdcl3/cd3od ) :  3.01 ( dd , j = 14.2 hz , j = 6.4 hz , 1h ) , 3.14 ( dd , j = 13.7 hz , j = 5.3 hz , 1h ) , 4.19 ( t , j = 6.6 hz , 1h ) , 4.274.49 ( m , 2h ) , 4.504.62 ( m , 1h ) , 5.82 ( d , j = 8.4 hz , 1h ) , 6.89 ( d , j = 8.2 hz , 2h ) , 7.277.47 ( m , 4h ) , 7.517.64 ( m , 4h ) , 7.78 ( d , j = 7.4 hz , 2h ) ; lc / ms calcd for c24h21ino4 [ m + h ] 514.0 ; found 514.0 . 5.9 mg ( 27% isolated yield ) following chromatographic purification ( ch2cl2/meoh / h2o , 300:10:0.5 ) ; initial lc / ms purity 86% , tr = 5.57 min ; h nmr ( 200 mhz , cdcl3/cd3od )  3.383.50 ( m , 1h ) , 3.75 ( dd , j = 14.2 hz , 5.4 hz , 1h ) , 4.084.22 ( m , 1h ) , 4.234.39 ( m , 2h ) , 4.73 ( dd , j = 7.4 hz , 5.6 hz , 1h ) , 7.237.55 ( m , 10h ) , 7.707.89 ( m , 4h ) , 8.16 ( d , j = 7.8 hz , 1h ) ; lc / ms calcd for c28h24no4 [ m + h ] 438.2 ; found 438.2 . 8.8 mg ( 40% isolated yield ) following chromatographic purification ( ch2cl2/meoh / h2o , 300:10:0.5 ) ; initial lc / ms purity 85% , tr = 5.58 min;h nmr ( 200 mhz , cdcl3/cd3od )  3.24 ( dd , j = 14 hz , j = 6.8 hz , 1h ) , 3.313.45 ( m , 1h ) , 4.124.45 ( m , 1h ) , 4.274.46 ( m , 2h ) , 4.69 ( t , j = 5.6 hz , 1h ) , 7.197.56 ( m , 10h ) , 7.64 ( m , 1h ) , 7.717.83 ( m , 4h ) ; lc / ms calcd for c28h24no4 [ m + h ] 438.2 ; found 438.2 . 10.2 mg ( 60% isolated yield ) following chromatographic purification ( ch2cl2/meoh / h2o , 300:10:0.5 ) ; initial lc / ms purity 93% , tr = 4.74 min ; h nmr ( 200 mhz , cdcl3/cd3od )  2.452.71 ( m , 2h ) , 4.23 ( t , j = 6.7 hz , 1h ) , 4.304.49 ( m , 3h ) , 5.055.25 ( m , 2h ) , 5.73 ( m , 1h ) , 7.297.46 ( m , 4h ) , 7.61 ( d , j = 7.2 hz , 2h ) , 7.77 ( d , j = 6.6 hz , 2h ) ; lc / ms calcd for c20h20no4 [ m + na ] 360.1 ; found 360.1 . 9.6 mg ( 44% isolated yield ) following chromatographic purification ( ch2cl2/meoh / h2o , 300:10:0.5 ) ; initial lc / ms purity 91% , tr = 5.00 min ; h nmr ( 200 mhz , cdcl3/cd3od )  3.25 ( dd , j = 13.2 hz , 9.4 hz , 1h ) , 3.63 ( dd , j = 13.9 hz , 5.5 hz , 1h ) , 4.064.20 ( m , 1h ) , 4.204.37 ( m , 2h ) , 4.70 ( dd , j = 8.6 hz , 5.4 hz , 1h ) , 7.277.59 ( m , 9h ) , 7.75 ( d , j = 7.2 hz , 2h ) , 7.94 ( d , j = 8.0 hz , 1h ) ; lc / ms calcd for c24h21n2o6 [ m + h ] 433.1 ; found 433.2 . 8.9 mg ( 39% isolated yield ) following chromatographic purification ( ch2cl2/meoh / h2o , 300:10:0.5 ) ; initial lc / ms purity 89% , tr = 5.71 min ; h nmr ( 200 mhz , cdcl3/cd3od )  3.07 ( dd , j = 13.6 hz , 8.8 hz , 1h ) , 3.323.46 ( m , 1h ) , 4.16 ( m , 1h ) , 4.224.42 ( m , 2h ) , 4.62 ( dd , j = 8.4 hz , 5.4 hz , 1h ) , 7.16 ( s , 2h ) , 7.287.45 ( m , 5h ) , 7.497.61 ( m , 2h ) , 7.76 ( d , j = 6.8 hz , 2h ) ; lc / ms calcd for c24h20cl2no4 [ m + h ] 456.1 ; found 456.2 . 10.3 mg ( 49% isolated yield ) following chromatographic purification ( ch2cl2/meoh / h2o , 300:10:0.5 ) ; initial lc / ms purity 94% , tr = 5.08 min ; h nmr ( 200 mhz , cdcl3/cd3od )  3.03 ( dd , j = 13.9 hz , 6.7 hz , 1h ) , 3.34 ( dd , j = 13.9 hz , 5.5 hz , 1h ) , 3.77 ( s , 3h ) , 4.19 ( t , j = 6.9 hz , 1h ) , 4.254.49 ( m , 2h ) , 4.56 ( t , j = 5.5 hz , 1h ) , 6.82 ( d , j = 7.6 hz , 2h ) , 7.07 ( d , j = 8.0 hz , 2h ) , 7.297.48 ( m , 4h ) , 7.527.64 ( m , 2h ) , 7.77 ( d , j = 7.2 hz , 2h ) ; lc / ms calcd for c25h24no5 [ m + h ] 418.2 ; found 418.2 . 7.9 mg ( 35% isolated yield ) following chromatographic purification ( ch2cl2/meoh / h2o , 300:10:0.5 ) ; initial lc / ms purity 84% , tr = 5.25 min ; h nmr ( 200 mhz , cdcl3/cd3od )  2.94 ( dd , j = 16.7 hz , 4.5 hz , 1h ) , 3.05 ( dd , j = 17.2 hz , 5.2 hz , 1h ) , 4.22 ( t , j = 7.1 hz , 1h ) , 4.294.45 ( m , 2h ) , 4.62 ( m , 1h ) , 5.15 ( s , 2h ) , 6.18 ( d , j = 8.0 hz , 1h ) , 7.267.46 ( m , 9h ) , 7.60 ( d , j = 7.4 hz , 2h ) , 7.76 ( d , j = 7.4 hz , 2h ) ; lc / ms calcd for c26h24no6 [ m + h ] 446.2 ; found 446.2 . 5.6 mg ( 27% isolated yield ) following chromatographic purification ( ch2cl2/meoh / h2o , 300:10:0.5 ) initial lc / ms purity 89% , tr = 5.14 min ; h nmr ( 200 mhz , cdcl3/cd3od )  3.003.26 ( m , 2h ) , 3.76 ( s , 3h ) , 4.144.46 ( m , 3h ) , 4.57 ( m , 1h ) , 6.736.83 ( m , 2h ) , 7.147.46 ( m , 6h ) , 7.527.62 ( m , 2h ) , 7.76 ( d , j = 7.0 hz , 2h ) ; lc / ms calcd for c25h24no5 [ m + h ] 418.2 ; found 418.2 . 5.5 mg ( 26% isolated yield ) following chromatographic purification ( ch2cl2/meoh / h2o , 300:10:0.5 ) ; initial lc / ms purity 80% , tr = 2.53 min ; h nmr ( 200 mhz , cdcl3/cd3od )  2.753.00 ( m , 2 h ) , 3.95 ( t , j = 6.4 hz , 1 h ) , 4.064.38 ( m , 3 h ) , 6.947.22 ( m , 6 h ) , 7.32 ( d , j = 7.2 hz , 2 h ) , 7.52 ( d , j = 7.0 hz , 2 h ) , 7.90 ( s , 1 h ) ; lc / ms calcd for c23h19cln2o4 [ m  h ] 421.1 ; found 421.0 .
distributed drug discovery ( d3 ) proposes solving large drug discovery problems by breaking them into smaller units for processing at multiple sites . 
 a key component of the synthetic and computational stages of d3 is the global rehearsal of prospective reagents and their subsequent use in the creation of virtual catalogs of molecules accessible by simple , inexpensive combinatorial chemistry . 
 the first section of this article documents the feasibility of the synthetic component of distributed drug discovery . 
 twenty - four alkylating agents were rehearsed in the united states , poland , russia , and spain , for their utility in the synthesis of resin - bound unnatural amino acids 1 , key intermediates in many combinatorial chemistry procedures . 
 this global reagent rehearsal , coupled to virtual library generation , increases the likelihood that any member of that virtual library can be made . 
 it facilitates the realistic integration of worldwide virtual d3 catalog computational analysis with synthesis . the second part of this article describes the creation of the first virtual d3 catalog . 
 it reports the enumeration of 24  416 acylated unnatural amino acids 5 , assembled from lists of either rehearsed or well - precedented alkylating and acylating reagents , and describes how the resulting catalog can be freely accessed , searched , and downloaded by the scientific community .