apesar dos sistemas adesivos autocondicionantes ( saa ) serem indicados para aplicao no esmalte dental , preocupao tem sido relatada com relao a sua efetividade . o objetivo deste estudo foi avaliar o padro de condicionamento cido ( pca ) promovido por nove saa e comparar ao pca produzido pelo cido fosfrico ( 35% e 34% - af ) no esmalte intacto ( ei ) ou abrasionado ( ea ) . vinte e dois terceiros molares humanos foram seccionados nos sentidos msio - distal e vestbulo - lingual , e quatro fragmentos dentais foram obtidos a partir de cada dente . metade dos fragmentos tiveram o esmalte abrasionado com lixas de sic ( 600 ) e a outra metade permaneceu intacta . os fragmentos foram divididos em 22 grupos , de acordo com a textura da superfcie do esmalte ( ei e ea ) e a tcnica de condicionar o esmalte ( af 34 % , af 35% , adhese primer ; brush & bond ; clearfil protect bond primer ; ibond ; one - up bond f ; optibond solo plus primer ; tyrian spe primer ; unifil bond primer e xeno iii ) . os agentes condicionadores foram aplicados nos ei e ea , de acordo com as instrues dos fabricantes . espcimes tratados com af foram lavados com gua , enquanto os dentes tratados com saa foram tratados com banhos alternados de lcool e acetona . em ambas as superfcies ( ei e ea ) , o pca dos af ( 34 e 35% ) foi mais profundo e homogneo , quando comparados ao pca produzido pelos saa , exceto para o adesivo tyrian spe . a maioria dos saa  menos agressiva que o cido fosfrico e seus efeitos condicionadores so reduzidos em superfcies de ei . buonocore2 ( 1955 ) reported that bonding to enamel surface could be increased by conditioning the surface with 85% phosphoric acid for 30 seconds . since this study , a proper enamel conditioning produces a selective dissolution , resulting in one of three etching patterns . phosphoric acid etching removes approximately 10 m of the enamel surface , creating a porous layer and increasing the surface energy and wettability25,27 . although the adhesion to enamel produced by phosphoric acid etching has been considered stronger and more durable , self - etching adhesives are alternative methods to prepare the tooth for restorative procedures . these adhesive systems have simplified clinical use because they do not require separated phosphoric acid etching , water rinsing or superficial moist controlling steps . however , studies evaluating self - etching adhesives are in disagreement regarding the efficacy of conditioning and monomer infiltration on enamel10,13,17,18,20 . morphological analyses of enamel surface treated with self - etching primers have shown not very demineralized surfaces and other areas that were predominantly unetched14,16,29 . in an attempt to improve the bonding of self - etching systems to enamel , surface pre - treatments , increase in acidic monomer concentration , increase of etching time as well as different application methods the purpose of this in vitro study was to compare the effects of self - etching adhesive systems and conventional phosphoric acid etchants on the morphology and the acid etching pattern of intact and ground enamel . the hypothesis was that the self - etching systems do not etch the ground and intact enamel surfaces such as phosphoric acid gels . twenty - two extracted , caries - free erupted human third molars were used in this study according to protocols approved by the institutional review board of the piracicaba school of dentistry teeth were obtained from patients from 19 to 25 years old and stored in saline with 0.1% thymol for no longer than 3 months . tooth roots were severed and the crowns were longitudinally sectioned ( mesio - distally and buccal - lingually directions ) into four quarters , using a diamond blade ( isomet , buehler ltd . , lake bluff , il , usa ) under water cooling . the buccal or the lingual flat enamel surface of each fragment was chosen and selected for conditioning treatments and bonding procedures . all specimens were randomly assigned to twenty - two groups ( n = 4 ) , according to surface treatment of enamel ( ground and unground ) and type of acid etching ( self - etching and phosphoric acid ) . the dental fragments from the ground surface groups had their enamel surface abraded with a # 600-grit sic paper on a polishing machine ( apl-4 , arotec s.a . experimental groups comprised enamel treatments with two phosphoric acid concentrations : 35% ( 3 m espe , st . paul , mn , usa ) and 34% ( dentsply caulk , milford , de , usa ) ; five acidic primers of two - step self - priming systems ( adhese - ivoclar vivadent , schaan , liechtenstein ; clearfil protect bond - kuraray medical inc . , kurashiki , okayama , japan ; optibond solo plus self - etch - kerr corp . , orange , ca , usa ; unifil bond - gc corp . , , schaumburg , il , usa ) and four one - step self - etching adhesives ( brush & bond - parkell inc . , edgewood , ny , usa ; i - bond - heraeus kulzer , hanau , germany ; one - up bond f - tokuyama dental corp . , taitou- ku , tokyo , japan , xeno iii - dentsply detrey , konstanz , germany ) . phosphoric acids were applied to the ground and intact enamel surfaces and left undisturbed for 15 seconds , rinsed with water spray for 30 seconds and dried for 30 seconds . the composition , manufacturers and directions of self - adhesive systems are described in table 1 . procedures  a : air - dry ; b : apply primer ; c : leave undisturbed ; d : gently air  dry ; e : mix liquid a and b ; f : apply mixture . specimens treated with self - etching primers were thoroughly rinsed with acetone and ethanol solutions in order to dissolve and remove the self - etching primer and adhesive resins5 . all specimens were dried , dehydrated in a desiccator for 12 hours and treated enamel surfaces were sputter - coated with gold / palladium in a vacuum evaporator ( scd 050 , balzers , schaan , liechtenstein ) . the entire surface of treated enamel was examined under a scanning electron microscope ( jsm 5600lv , jeol , tokyo , japan ) , however , only photomicrographs of representative surface areas were taken at 10,000x magnification . phosphoric acid etchants changed the enamel morphology for both intact and ground surfaces ( figures 1 and 2 ) . dissolution of prism cores and boundary regions can be observed , however , the conditioning of enamel surface was not uniform along the unground surfaces ( figure 2b ) . no distinct morphological differences between the phosphoric acid concentrations ( 34% and 35% ) were observed on the etched enamel surfaces . figures 3 to 11 represent the morphology of enamel surface treated with self - etching systems . the etching patterns ranged from mild demineralization to an aspect of surfaces etched with phosphoric acid . tyrian spe self - etching primer produced dissolution of enamel surface , exposing enamel crystallites , which resulted in an etching pattern similar to that created by phosphoric acid etchants ( figure 9 ) . mild demineralization was promoted by brush & bond , one - up bond f , clearfil protect bond and unifil bond self - etching adhesives ( figures 4 , 5 , 7 and 10 ) , whereas adhese , ibond , optibond solo plus and xeno iii ( figures 3 , 6 , 8 and 11 ) resulted in moderate mineral dissolution . ground enamel allowed the self - etching primers to condition the subsurface enamel , exposing crystallites when the etching pattern was considered at least moderate ( figures 3a , 6a , 8a , 9a , 11a ) . acidic monomers have been developed containing esters from phosphoric acid , carboxylic acid or derivatives12,14,18,21 . their etching efficacy depends on acidic monomer , ph of adhesive solution , etching time and application method3,16,18 . they are responsible for etching the dental substrates , whereas methacrylate components , such as hema , are available for monomer infiltration and polymerization of the bonding agent8,10,12,13 . as the application of self - etching adhesives comprises simplified bonding procedures , there have been concerns regarding the longevity of bonding , according to some in vitro and in vivo results4,15,24 . studies have shown that most of the self - etching adhesives did not etch enamel as deeply as the phosphoric acid etchants did and the shallow etching pattern could compromise the bonding to enamel6,10,15,21 . pashley and tay18 ( 2001 ) reported that the efficacy of self - etching primers in intact enamel does not depend solely upon their etching aggressiveness , but also on monomeric composition of each material . it is also possible that the low enamel bond strengths might be caused by the high amount of unpolymerized acidic monomers remaining after curing13 . thus , no correlation among degree of primer aggressiveness , enamel etching pattern and bond strength to unground enamel has been reported for self - etching adhesives9,23 . the etching effect of phosphoric acid etchants was similar to that previously described by retief25 ( 1973 ) and silverstone , et al.27 ( 1975 ) . the prism cores and boundaries were etched by 34% and 35% phosphoric acids , causing dissolution of both inter and intraprismatic areas . the predominant etching pattern was type 2 , which has the peripheral region of prisms removed and prism cores relatively unaffected ( figures 1a and 2a ) . the unground enamel treated with phosphoric acids also showed formation of a porous surface , exhibiting the exposed enamel crystallites along the entire surface ( figures . figure 2b shows the remnants of aprismatic or prismless layer that was partially dissolved by phosphoric acid etching . in this current study , as enamel surfaces were obtained from third molars , it was possible that the treated outer enamel layer would be prismless22 . although phosphoric acid etchants present ph below 119 , unground enamel surfaces were not totally attacked or conditioned by acid etching . the ph values of all self - etching systems tested were higher than that for phosphoric acid . in general , the demineralization effects of these systems were proportional to the acidity of the acidic primers or self - etching adhesive solutions . the self - etching primers were less aggressive than phosphoric acid etchants and the conditioning effects were also reduced in unground enamel surfaces , except for tyrian spe self - etching primer . this result is in agreement with other studies that demonstrated that self - etching adhesives do not form a proper and defined acid etching pattern in intact surfaces5,10,14,21,23 . sem observations indicated that only shallow pits were produced after some self - etching treatments in intact enamel ( figures 6b , 8b , 10b and 11b ) . the removal of superficial , aprismatic layer by wet - grinding with 600-grit sic paper improved the etching effects . the morphological structure and composition of the intact peripheral surface of enamel is different from that of the middle enamel layer22 . these differences can be favorable for etching effects in subsurface enamel . for tyrian spe primer , the etching pattern on the ground surface was similar to phosphoric acid - etched enamel ( figure 9a ) . however , the aggressiveness of the acidic primer has not ensured that consistent bonding may be established13,18,26 . examination of ground enamel surfaces treated with brush & bond , clearfil protect bond , one - up bond f and unifil bond self - etching systems ( figures 4a , 5a , 7a and 10a ) revealed that the surfaces were predominantly unetched . adhese , ibond , optibond bond solo plus and xeno iii self - etching systems resulted in moderate and particular etching pattern that comprised demineralization of the surface with exposure of the enamel crystallites ( figures 3a , 6a , 8a and 11a ) . the self - etching primer mechanism ofbonding to enamel is based on nanoretentive interlocking between crystallites and adhesive resin9,26 . these morphological features of the resin - enamel bonds are different from that formed with the etch&rinse adhesive systems9,11,23,26 . this thin hybridized complex of resin in enamel produced by self - etching without the usual micrometer - size resin tags can be responsible for lower bond strength and questionable effectiveness on enamel surfaces1,28,30 . based on scientific evidence , some authors have recommended the instrumentation of enamel before bonding , in attempt to increase the bond strength6,14,21,23 . thus , resin bond strength achieved with self - etching systems are sometimes comparable to those achieved with phosphoric acid , despite the differences between enamel etching pattems8,12,13,14,20 . however , the controversy about the effectiveness still remain , since other studies did not show the same results of bond strength1,6,11,28,30 . regarding acid etching technique , it is well established that phosphoric acid provides good adhesion to both ground and unground enamel2,19,25,27 . because the action of self - etching primers resulted in much less demineralization of intact enamel surfaces , enamel abrasion during cavity preparation can favor the formation of a defined etching pattern . since in clinical situations the enamel is usually prepared with dental drills prior to application of the adhesive system however , the effects of self - etching adhesive systems must be further studied to verify the durability of bonding to enamel . the self - etching systems did not etch the ground and intact enamel surfaces as phosphoric acid etchants did . their conditioning effects were reduced on intact enamel surfaces , except for tyrian spe system . the acid - etched enamel patterns formed by acidic monomers were observed only on ground surfaces .
objective : although self - etching bonding systems ( ses ) are indicated to prepare dental enamel for bonding , concerns have been expressed regarding their effectiveness . the aim of this study was to analyze the etching pattern ( ep ) of nine ses in comparison with 35% and 34% phosphoric acid etchants ( fa ) on intact ( in ) and ground ( gr ) enamel surface.materials and methods : twenty - two human third molars were sectioned in mesial - distal and buccal - lingual directions , and four dental fragments were obtained from each tooth . 
 half of the fragments were ground using 600-grit sic paper and the other half remained intact . 
 the fragments were randomly assigned into 22 groups , according to the texture of enamel surface ( in and gr ) and the technique to etch the enamel ( 34% fa , 35% fa , adhese primer ; brush & bond ; clearfil protect bond primer ; ibond ; one - up bond f ; optibond solo plus primer ; tyrian spe primer ; unifil bond primer and xeno iii ) . 
 conditioners were applied to in and gr enamel surfaces , according to the manufacturer 's instructions . 
 specimens etched with phosphoric acids were washed with water , while the surfaces treated with ses were submitted to alternate rinsing with alcohol and acetone . 
 the specimens were dried , sputter - coated and examined under a scanning electron microscope.results:for both in and gr enamel surfaces , the ep of 34 and 35% fa was deeper and more homogeneous in comparison to ep of ses , except for tyrian spe . 
 the acidic monomer action of self - etching systems was more effective on gr enamel.conclusion:most of the ses are less aggressive than phosphoric acid etchants and their etching effects were reduced on intact enamel surfaces . 
 uniterms : dental acid etching ; dental enamel ; electron microscopy .