since 2006 , surveillance physicians have listed and collected blood from those patients encephalitis , defined as fever or history of fever with axillary temperature > 38.5c ( 101.3f ) and altered mental status , new onset of seizures , or new neurologic deficit  in patients admitted to 3 nipah surveillance hospitals : rajshahi , rangpur , and faridpur medical college hospitals . the institute for epidemiology disease control and research and us centers for disease control and prevention tested serum with an igm - capture enzyme immunoassay to detect niv igm , and we defined laboratory - confirmed niv encephalitis as niv igm in serum . during december 2012march 2013 , surveillance physicians interviewed accompanying caregivers of all hospitalized patients whose illness met the encephalitis case definition on admission in the inpatient ward . study physicians asked about patients consumption of raw or fermented date palm sap and contact with other persons with fever and altered mental status in the month before illness onset ; if caregivers were unaware of the patient s exposures , study physicians asked them phone the patient s friends and colleagues about exposures . hospital physicians used personal protection equipment and provided it to caregivers of each patient with encephalitis and a history of these exposures . as part of subsequent epidemiologic studies , we also conducted detailed case investigations at each niv encephalitis case - patient s household . we interviewed surviving patients directly , or appropriate proxies among family , friends and relatives for patients who died , about their exposures to encephalitis patients or to fresh or fermented date palm sap before illness . we calculated the sensitivity , specificity , positive predictive value ( ppv ) , and negative predictive value ( npv ) of the screening questions asked on admissions to hospitals by comparing with the niv igm results . we repeated the calculations for patients hospitalized during january and february , when the prevalence of niv encephalitis is highest . we compared the answers provided by caregivers during patient hospitalization with those provided during interviews in the community as part of our epidemiologic studies . icddr , b s ethical review committee reviewed and approved the protocol for niv surveillance and case investigation . they collected and tested blood samples from 328 ( 91% ) patients for niv igm . seventeen ( 5% ) had niv igm ( table 1 ) , of whom 15 ( 88% ) niv encephalitis case - patients were identified during january and february 2013 . of the 17 confirmed case - patients , family caregivers of 14 reported either a history of drinking raw or fermented date palm sap or contact with other persons with fever and altered mental status in the month before illness onset . therefore , the sensitivity of the screening questions was 82% , specificity was 86% , ppv was 24% and npv was 99% ( table 2 ) . the sensitivity during january  february was 93% , specificity was 82% , ppv was 37% , and npv was 99% . * drinking raw or fermented date palm sap or having contact with encephalitis patients in month before illness onset . at admission , 3 ( 18% ) niv encephalitis case - patients had no reported history of drinking raw or fermented date palm sap or of contact with persons who had encephalitis ( table 1 ) . however , during the epidemiologic investigations in the community , family members of 2 case - patients reported that the patients drank fermented date palm sap in the month before illness onset . of the 14 niv encephalitis case - patients who , at admission , had reported 1 of the risk exposures , results were consistent with exposures reported during the epidemiologic investigation . screening patients with possible encephalitis at the time they seek hospital care regarding recent exposure to date palm sap and to other patients with encephalitis demonstrated high sensitivity and specificity for detecting niv encephalitis , particularly during peak months of niv encephalitis incidence . the high npv of the screening questions suggests that focusing infection control efforts toward patients with these exposures is an efficient use of scarce resources to prevent transmission . although three fourths of encephalitis patients had reported histories of exposure , they possibly could have had other infections , including other bat - borne viruses , that were transmitted through similar routes or could have lacked niv igm , despite having niv infection ( 13 ) . alternatively , recent consumption of date palm sap by these patients might have been purely coincidental because this practice is common in bangladesh during this season , but nipah infection is rare . for 2 niv encephalitis case - patients , caregivers did not report a history of drinking fermented date palm sap during hospital interview , but this behavior was reported in later community investigations . because 90% of bangladeshis are muslim , and consumption of alcohol is prohibited by islam ( 14 ) and illegal in bangladesh , patients might be reluctant to report drinking traditional liquor made of fermented date palm sap . therefore , caregivers should be asked about socially stigmatized behaviors privately and confidentially to increase the odds that these stigmatized behaviors are reported . exposure - based screening can detect patients at high risk for niv encephalitis in low - income , resource - constrained settings , such as bangladesh . we deployed screening questions on admission to inpatient wards but screening earlier , at triage in emergency wards , could further reduce risk . surveillance for other diseases with well - described exposures that put healthcare workers at risk , such as ebola virus infection , and where laboratory diagnosis is limited or delayed could also deploy this approach .
we measured the performance of exposure screening questions to identify nipah virus encephalitis in hospitalized encephalitis patients during the 201213 nipah virus season in bangladesh . the sensitivity ( 93% ) , 
 specificity ( 82% ) , positive predictive value ( 37% ) , and negative predictive value ( 99% ) results suggested that screening questions could more quickly identify persons with nipah virus encephalitis .