gram - positive cocci are impor tant pathogens which can specially cause soft tissue and skin infection ( 1 ) . s. aureus and coagulase - negative staphylococci ( cons ) are recognized as common microorganisms leading to nosocomial or community acquired infection all over the world ( 2 , 3 ) . increased incidence of methicillin resistance among staphylococci is a growing problem ( 1 ) and they are commonly reported as multi - drug resistant ( mdr ) microorganisms ( 1 , 2 , 4 ) . this fact has changed the trends in the usage of macrolide , lincosamide and streptogramin b ( mlsb ) antibiotics in the treatment of staphylococcal infections ( 3 , 5 ) . the mlsb antibiotics are a family , reserved as alternatives in the treatment of resistant gram positive cocci ( staphylococci and streptococci ) ( 1 ) . although they are structurally different , their mode of action is similar ( 1 , 4 ) . these antibiotic share common binding sites thus called mlsb phenotype ( 2 ) . clindamycin has been considered as the preferred agent due to its excellent pharmacokinetic properties ( 1 , 2 , 4 , 5 ) including good penetration and distribution in to the skin and other soft tissue structures ( 6 ) and acceptable oral absorption with no dosage adjustment in renal disorders ( 1 ) . however regarding wide spread use of this antibiotic , increasing number of resistant isolates are being developed ( 1 , 7 ) . firstly , it can be mediated by msr(a ) gene encoding efflux pump and secondly by a variety of erm genes coding enzymes that confer inducible or constitutive resistance to mlsb agents ( through methylation of the 23sr rna ) ( 1 , 3 , 4 ) . constitutive resistance can normally be detected by routine standard susceptibility testing whereas strains that demonstrate inducible resistance can not be recognized by routine broth or agar based susceptibility tests ( 1 , 3 , 8) . in such cases treatment with clindamycin may lead to clinical failure by developing constitutive resistant microorganisms ( 1 , 5 , 9 ) . the presence of inducible clindamycin resistance can appropriately be recognized with d - zone test ( 9 ) . this study was designed to determine the frequency of inducible clindamycin resistance among staphylococci and -hemolytic streptococci in our hospital , as a tertiary center , to highlight the necessity of performing d - test in routine practice in order to avoid reporting false susceptible results . also , we aimed to evaluate any possible correlation between the frequency of inducible clindamycin resistance and susceptibility pattern to methicillin in staphylococci . total number of 487 consecutive non duplicate isolates of staphylococci and -hemolytic streptococci group b and spp . were recovered from various clinical specimens during march to september 2014 at microbiology laboratory of shariati hospital , tehran , iran . during this period , 172 , 277 , 23 and 15 isolates of s. aureus , coagulase - negative staphylococci and -hemolytic group b streptococci and spp . were identified , respectively . identification was done based on colony morphology on 5% blood agar , gram staining and further conventional biochemical tests ( 10 ) . staphylococci and -hemolytic streptococci isolates were evaluated for the pattern of erythromycin susceptibility using kirby - bauer disk diffusion method on muller hinton agar with or without blood supplement . all erythromycin resistant and clindamycin sensitive isolates were subjected to d - zone test using erythromycin ( 15 g ; rosco diagnostica , denmark ) and clindamycin ( 2 g ; rosco diagnostica , denmark ) according to clinical and laboratory standards institute ( clsi ) ( 11 ) . briefly , erythromycin disks were placed at a distance of 15 mm and 12 mm ( edge to edge ) from clindamycin disk ( for staphylococci and streptococci , respectively ) on plates inoculated with the bacterial suspension with adjusted turbidity to mcfarland standard 0.5 followed by overnight incubation at 37 c . the results were interpreted as follows ( 1 ) ; ms phenotype , sensitive to clindamycin with circular zone of inhibition around the disk ; imlsb ( inducible resistance ) phenotype , sensitive to clindamycin with a d - shaped zone of inhibition around clindamycin disk ; cmlsb ( constitutive resistance ) phenotype , resistant to clindamycin with a circular shape of inhibition . resistance to methicillin in staphylococci was detected by disk diffusion method using cefoxitin disk ( 30 g ; rosco diagnostica , denmark ) according to clsi ( 11 ) . complementary quality control for d - test also was done with selected in - house strain that was d - test positive . comparison of non - parametric quantitative variables was performed by mann - whitney u test while chi - square and fisher s exact test was utilized for analyzing qualitative data . total number of 487 consecutive non duplicate isolates of staphylococci and -hemolytic streptococci group b and spp . were recovered from various clinical specimens during march to september 2014 at microbiology laboratory of shariati hospital , tehran , iran . during this period , 172 , 277 , 23 and 15 isolates of s. aureus , coagulase - negative staphylococci and -hemolytic group b streptococci and spp . were identified , respectively . identification was done based on colony morphology on 5% blood agar , gram staining and further conventional biochemical tests ( 10 ) . staphylococci and -hemolytic streptococci isolates were evaluated for the pattern of erythromycin susceptibility using kirby - bauer disk diffusion method on muller hinton agar with or without blood supplement . all erythromycin resistant and clindamycin sensitive isolates were subjected to d - zone test using erythromycin ( 15 g ; rosco diagnostica , denmark ) and clindamycin ( 2 g ; rosco diagnostica , denmark ) according to clinical and laboratory standards institute ( clsi ) ( 11 ) . briefly , erythromycin disks were placed at a distance of 15 mm and 12 mm ( edge to edge ) from clindamycin disk ( for staphylococci and streptococci , respectively ) on plates inoculated with the bacterial suspension with adjusted turbidity to mcfarland standard 0.5 followed by overnight incubation at 37 c . the results were interpreted as follows ( 1 ) ; ms phenotype , sensitive to clindamycin with circular zone of inhibition around the disk ; imlsb ( inducible resistance ) phenotype , sensitive to clindamycin with a d - shaped zone of inhibition around clindamycin disk ; cmlsb ( constitutive resistance ) phenotype , resistant to clindamycin with a circular shape of inhibition . resistance to methicillin in staphylococci was detected by disk diffusion method using cefoxitin disk ( 30 g ; rosco diagnostica , denmark ) according to clsi ( 11 ) . complementary quality control for d - test also was done with selected in - house strain that was d - test positive . comparison of non - parametric quantitative variables was performed by mann - whitney u test while chi - square and fisher s exact test was utilized for analyzing qualitative data . total number of 487 microorganisms from different clinical samples was subjected to the study including 172 ( 35.3% ) s. aureus , 277 ( 56.9% ) coagulase - negative staphylococcus isolates , 23 ( 4.7% ) streptococcus group b and 15 ( 3.1% ) streptococcus spp . eighty six out of 172 ( 50% ) s. aureus isolates and 174/277 ( 62.8% ) con staphylococci were categorized as methicillin - resistant . erythromycin resistance was detected in 100/172 ( 58.1% ) of s. aureus isolates and 214/277 ( 77.25% ) con staphylococci . the frequency of susceptibility pattern to erythromycin as well as different patterns of susceptibility to clindamycin in both s. aureus and con staphylococci are summarized in table 1 . we did not observe any significant difference between inducible clindamycin resistance and methicillin susceptibility pattern . frequency of different patterns of susceptibility to erythromycin and clindamycin in staphylococcus isolates frequency of inducible clindamycin resistance in staphylococci regarding to methicillin susceptibility methicillin - resistant 

 s. aureus 
  methicillin - susceptible 

 s. aureus 
  methicillin - resistant con staphylococci methicillin - susceptible con staphylococci we also performed d - test in -hemolytic streptococci ( group b and other streptococcus spp . ) . frequency of susceptibility patterns towards erythromycin and clindamycin in streptococci we also determined the frequency of inducible clindamycin resistance in different hospital wards and clinical samples . medical wards were categorized as emergency ( general , oncology , obstetrics ) , internal medicine ( nephrology , respiratory , general , renal transplant , endocrinology , gastrointestinal , heart and ccu ) , surgical wards ( general , orthopedics , urology , neurosurgery , gynecology , head and neck ) , intensive care units ( general , nicu , neurosurgery ) and hematology / oncology / bone marrow transplant . data are summarized in tables 4 and 5 , respectively . considering the number of submitted samples , the most frequent inducible resistant microorganisms were retrieved from internal medicine wards . in regard to the number of samples frequency of inducible clindamycin resistance regarding to different hospital wards frequency of inducible clindamycin resistance regarding to different clinical specimens accurate designation of antimicrobial susceptibility pattern of the infecting microorganisms is an important crucial factor in making appropriate therapeutic decisions ( 2 , 12 ) . regarding the emergence of resistance in gram positive cocci , especially staphylococci , various antibiotics have been considered as alternative therapeutic options . the macrolide , lincosamide and streptogramin b ( mlsb ) family including clindamycin as one of the best preferred agents serves as one of these alternatives ( 2 ) . clindamycin has good oral bio - availability and is helpful for outpatient therapy or as oral agent can be followed after intravenous therapy ( 2 ) . clindamycin also has good penetration into skin or soft tissues or may be able to inhibit production of some toxins or virulence factors by staphylococci and is cost effective as well ( 46 , 13 ) . the widespread use of this family of antibiotics has led to the development of resistant strains ( 7 , 14 ) . the common mechanism of resistance is via erm gene encoding for enzymes confer constitutive or inducible resistance to mls 

 b 

 family ( 7 , 1517 ) . routine in vitro susceptibility tests may fail to detect inducible resistance to clindamycin when erythromycin and clindamycin disks are not placed adjacent to each other thus false susceptible report may result in treatment failures ( 2 , 4 , 7 , 12 ) . the prevalence of inducible mlsb resistance varies in different geographic locations and is believed that depends on patient population , hospital characteristics and geographic area ( 3 ) . according to our findings , the frequency of erythromycin resistance was 69.93% among staphylococci ( 58.1% in s. aureus isolates and 77.25% in con staphylococci ) which is higher than the prevalence rates reported by others ( 1 , 4 , 5 , 18 ) . our data revealed the similar incidence rate of inducible clindamycin resistance among methicillin resistant staphylococcus aureus ( mrsa ) and methicillin sensitive staphylococcus aureus ( mssa ) isolates ( 7/86 and 6/86 strains , respectively ) . although this finding is the same as published reports in other parts of the world ( 4 , 19 ) but higher rate of inducible resistance among mrsa have also been reported ( 1 
 , 5 , 18 , 20 , 21 ) and even controversial results have been observed ( 3 , 22 , 23 ) . among coagulase negative staphylococci , 28/214 ( 13% ) of erythromycin resistant isolates showed inducible clindamycin resistance phenotype which is close to the 17% incidence rate reported by others ( 1 ) . the frequency of methicillin resistance in our study was 50% and 62.8% in staphylococcus aureus and con staphylococci , respectively . in a study by dibah et al . ( 24 ) the frequency of mrsa isolates in ardabil ( north west of iran ) was 46.3% and most of the specimens were isolated from icu . in another national study by pourmand ( 25 ) the frequency of mrsa was 50% using both cefoxitin disk diffusion method and pcr assay . both of these studies approximately supported our findings . in another study conducted in a burn center in ahvaz , higher frequency of methicillin resistance in staphylococcus aureus isolates was reported ( 60% ) while the rate of methicillin resistance among con staphylococcus strains was 63% ( 26 ) which was quite similar to the present study . for streptococcous agalactiae , the frequency of resistance towards erythromycin was about 34.78% which is near to the 40% frequency rate reported by hraoui et al . the rate of inducible and constitutive clindamycin resistance among erythromycin resistant microorganisms was 17.40 and 8.69% , respectively while hraoui et al . the emergence of antibiotic resistance , especially methicillin resistance in gram positive cocci , considers clindamycin as an acceptable alternative treatment option . according to our findings , considerable number of bacterial isolates in our center showed imbl pattern . because this type of resistance can not be recognized in routine tests , clsi recommends d - zone testing as a simple test which should be performed to avoid false susceptible results leading to treatment failure .
background and objectives : accurate designation of antimicrobial susceptibility pattern of the infecting microorganisms is an important crucial factor in making appropriate therapeutic decisions . 
 macrolide , lincosamide and streptogramin b antibiotics are in a family , reserved as an alternative approach in treatment of resistant gram positive cocci . amongst them , clindamycin has been considered as the preferred agent due to its excellent pharmacokinetic properties 
 . the inducible resistance to clindamycin in gram positive staphylococci and streptococci can not be recognized by routine broth or agar based susceptibility tests and d - zone testing is necessary . 
 this study is conducted to evaluate the frequency of inducible clindamycin resistance in gram positive cocci.materials and methods : using traditional culture methods , 487 isolates of staphylococcus and -hemolytic streptococcus were evaluated . 
 if they were resistant to erythromycin and sensitive to clindamycin in primary antibiotic susceptibility testing by kirby - bauer method , they were subjected to d - zone testing to detect possible inducible clindamycin resistance.results:thirty three out of 172 isolates of staphylococcus aureus and 50 out of 277 isolates of coagulase - negative staphylococci ( cons ) were subjected for d - zone testing . among them 
 13/33 and 28/50 showed inducible clindamycin resistance , respectively . 
 there was no significant difference in inducible clindamycin resistance regarding to methicillin susceptibility pattern . 
 positive d - test was observed in 17.39 and 13.33% of group b streptococci and streptococcus spp . , respectively.conclusion:considerable number of isolates showed inducible clindamycin resistance in our study which falsely would be reported susceptible if d - zone testing was not performed . 
 thus , performing d - zone testing is necessary to avoid misleading results which may cause treatment failure .