investigar si la precisin de las mediciones de la presin intraocular ( pio ) , utilizando la tonometra de rebote sobre las lentes de contacto ( lc ) desechables de hidrogel ( etafilcon a ) , se ve afectada por la potencia positiva de dichas lentes . el grupo experimental incluy a 26 sujetos , ( 8 varones , 18 mujeres ) . se realiz la medicin de la pio en los ojos derechos de los sujetos , de modo aleatorio , utilizando un tonmetro de rebote ( icare ) . las lc tenan potencias de + 2,00  d y + 6,00  d. se realizaron mediciones el valor de la pio obtenido con ambas lc fue considerablemente menor al valor sin lc ( t del test ; p  <  0,001 ) , aunque no se hall una diferencia significativa entre las dos potencias de las lentes . la tonometra de rebote sobre las lc positivas de hidrogel origina un cierto grado de subestimacin del pio . este resultado no sufri variacin entre las dos lentes positivas utilizadas en el experimento , a pesar de la gran diferencia de potencia , y por tanto del espesor de las lentes . los optometristas deberan de tener en cuenta estos resultados en a la hora de medir el pio con un tonmetro de rebote , con lentes de contacto de mayor potencia . primary open angle glaucoma is a potentially blinding condition . raised intraocular pressure ( iop ) is an important risk factor for the development and progression of optic nerve damage in glaucoma , and is the target of both medical and surgical treatment being currently the only treatable risk factor . measuring iop over a soft contact lens ( cl ) can be very useful for several reasons . these include avoiding topical anaesthesia , minimizing trauma in conditions of corneal pathology , whenever there is a need to undertake tonometry several times , when the corneal surface is extremely irregular and finally to allow iop measurement without removing the cls . villani was probably the first to use a soft contact lens with contact tonometry having the aim of avoiding pharmacological anaesthesia . several studies showed that measurement of iop can be performed over soft contact lenses using the goldmann tonometer,5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 the mackay marg tonometer , the tono - pen,14 , 15 , 16 , 17 the gas pneumotonometer,16 , 17 the non - contact tonometer18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 and the dynamic contour tonometer.11 , 27 , 28 , 29 in 2005 a new handheld device became available to measure iop , the icare rebound tonometer , having the advantage over other instruments that no topical anaesthesia is required . a light magnetized small , disposable probe , characterized by a round plastic tip , is launched towards the eye using a solenoid . the return rate of the probe after it touches the cornea permits information about iop.30 , 31 the results are reproducible and reasonably accurate.32 , 33 several investigators34 , 35 , 36 , 37 , 38 , 39 , 40 have evaluated the icare tonometer compared with other tonometry devices , showing a reasonable overall correlation and concordance between the iop obtained with the goldmann or pascal types . it has been shown that with the rebound tonometer it is possible to measure iop over soft cls , either hydrogel or silicone hydrogel , with good clinical accuracy.39 , 40 however it has been found that the type of material and the power of the cl can cause an underestimation of iop or overestimation.25 , 42 the aim of this study was to verify this effect not only for a + 2.00  d cl but also for a higher positive + 6.00  d cl . other corneal parameters such as thickness and curvature were evaluated to investigate their influence on the measurement . twenty - six subjects ( 8 male and 18 female ) , age range from 21.2 to 48.7 years ( mean 28.8 ; sd 8.9 years ) , were enrolled in the study . inclusion criteria were normal corneas ( no corneal scarring , corneal pathology or prior corneal surgery ) , assessed by slit lamp examination and videokeratoscopy , and corneal astigmatism of not more than 2.50  d. contact lens wearers were enrolled only if they had taken their lenses out for 12  h before the experiment . all subjects had been informed about the experiment in detail and had signed the consent document in compliance with the declaration of helsinki before the experiment . all tonometric measurements were carried out with a rebound tonometer ( icare ; finland oy ) . two different spherical powers were used : + 2.00  d and + 6.00  d. to evaluate the effect of power on the measurement of iop , a repeated measurements design was used . four measures of iop were taken on the right eye of each subject . the first measurement ( rt1 ) and the last measurement ( rt4 ) were taken without cls . the second and third measurements were performed over the two different powers of the cls . in order to prevent a possible effect on iop of the repetition of the measurement or the insertion and removal of cls , each subject was assigned randomly to one of two different sequences ( table 2 ) . in order to control accommodation , that might influence the measurement of iop during experiments,45 , 46 the left eye ( corrected with cls for any hyperopic defect ) viewed a distance target ( 6/24 or 0.6  logmar ) . one investigator assigned each subject randomly to one experimental condition and fitted all cls to each subject . a second investigator , experienced in rebound tonometry , performed all iop measurements on each subject for all conditions in order to reduce between - observer bias . a third investigator checked the position of the rebound tonometer probe on the cornea during the measurement . this control was performed because it has been demonstrated that the location of the tonometer on the cornea can affect the measurement of iop47 , 48 even though a recent study showed that the rebound tonometer appears insensitive to misalignments . after the measurement the third investigator read the measure on the display of the tonometer . thus the measurements were repeated up to the moment the third investigator had two valid readings . the number of measures required to achieve two valid readings was recorded . to reduce between observer and fitter bias all measurements were taken between 1.00 and 3.00 pm in order to minimize the effect of diurnal variation of iop on the results . before the iop measurements , a corneal topographic map as well as a pachymetric map of each cornea was taken by a scheimpflug camera system ( sirius acquiring system ; cso , florence , italy ) in order to evaluate a possible effect of corneal thickness and curvature on tonometric measurement . smirnov test was used to evaluate the results for a normal distribution of iop , and corneal parameters data . all the statistical processing used to analyze the comparison between the measurements with and without cls was performed using a value for the latter ( rt ) that was the mean of the first ( rt1 ) and last measurements ( rt4 ) . the strength of the relationship between iop measurements without cls and with the two powers of cls was evaluated using a correlation analysis ( r of pearson ) . altman plot was used to assess the difference in iop reading with and without the two powers of cls as a function of iop value . a student 's paired t test for repeated measurement was applied in order to evaluate the differences between the measurements obtained without cls and with each positive cl . considering the sample size , the statistical powers of the significant comparisons of paired t test + 2 and rt and + 6 and rt were 0.987 and 0.965 respectively . any possible relationship between corneal parameters ( thickness , curvature , asphericity ) and the measurement of iop over positive cls was evaluated using a correlation analysis ( r of pearson ) between every corneal parameter measure and the difference in iop measurement with and without the cls . twenty - six subjects ( 8 male and 18 female ) , age range from 21.2 to 48.7 years ( mean 28.8 ; sd 8.9 years ) , were enrolled in the study . inclusion criteria were normal corneas ( no corneal scarring , corneal pathology or prior corneal surgery ) , assessed by slit lamp examination and videokeratoscopy , and corneal astigmatism of not more than 2.50  d. contact lens wearers were enrolled only if they had taken their lenses out for 12  h before the experiment . all subjects had been informed about the experiment in detail and had signed the consent document in compliance with the declaration of helsinki before the experiment . all tonometric measurements were carried out with a rebound tonometer ( icare ; finland oy ) . the cls used were bi - weekly replacement hydrogel ( acuvue 2 ) . two different spherical powers were used : + 2.00  d and + 6.00  d. to evaluate the effect of power on the measurement of iop , a repeated measurements design was used . four measures of iop were taken on the right eye of each subject . the first measurement ( rt1 ) and the last measurement ( rt4 ) the second and third measurements were performed over the two different powers of the cls . in order to prevent a possible effect on iop of the repetition of the measurement or the insertion and removal of cls , each subject was assigned randomly to one of two different sequences ( table 2 ) . in order to control accommodation , that might influence the measurement of iop during experiments,45 , 46 the left eye ( corrected with cls for any hyperopic defect ) viewed a distance target ( 6/24 or 0.6  logmar ) . one investigator assigned each subject randomly to one experimental condition and fitted all cls to each subject . a second investigator , experienced in rebound tonometry , performed all iop measurements on each subject for all conditions in order to reduce between - observer bias . a third investigator checked the position of the rebound tonometer probe on the cornea during the measurement . this control was performed because it has been demonstrated that the location of the tonometer on the cornea can affect the measurement of iop47 , 48 even though a recent study showed that the rebound tonometer appears insensitive to misalignments . after the measurement the third investigator read the measure on the display of the tonometer . thus the measurements were repeated up to the moment the third investigator had two valid readings . the number of measures required to achieve two valid readings was recorded . to reduce between observer and fitter bias all measurements were taken between 1.00 and 3.00 pm in order to minimize the effect of diurnal variation of iop on the results . before the iop measurements , a corneal topographic map as well as a pachymetric map of each cornea was taken by a scheimpflug camera system ( sirius acquiring system ; cso , florence , italy ) in order to evaluate a possible effect of corneal thickness and curvature on tonometric measurement . data were analyzed using statistica ( statsoft inc . , tulsa , ok , usa ) v.6.0 for windows . smirnov test was used to evaluate the results for a normal distribution of iop , and corneal parameters data . all the statistical processing used to analyze the comparison between the measurements with and without cls was performed using a value for the latter ( rt ) that was the mean of the first ( rt1 ) and last measurements ( rt4 ) . the strength of the relationship between iop measurements without cls and with the two powers of cls was evaluated using a correlation analysis ( r of pearson ) . altman plot was used to assess the difference in iop reading with and without the two powers of cls as a function of iop value . a student 's paired t test for repeated measurement was applied in order to evaluate the differences between the measurements obtained without cls and with each positive cl . considering the sample size , the statistical powers of the significant comparisons of paired t test + 2 and rt and + 6 and rt were 0.987 and 0.965 respectively . any possible relationship between corneal parameters ( thickness , curvature , asphericity ) and the measurement of iop over positive cls was evaluated using a correlation analysis ( r of pearson ) between every corneal parameter measure and the difference in iop measurement with and without the cls . mean corneal astigmatism of the subjects right eyes was 0.73    0.37  d ( range 0.15/1.60  d ) . twenty right eyes had with the rule astigmatism ( steepest corneal meridian 90    20 ) , three had against the rule astigmatism ( steepest corneal meridian 180    20 ) and three had oblique astigmatism ( steepest meridian between 21 and 69 or 111 and 159 ) . the right eye central corneal thickness and the corneal thickness at the pupil centre was 540    32  m and 542    32  m respectively . mean spherical equivalent refraction of the subjects right eyes was 2.10    2.28  d ( range 0.75/7.13  d ) . mean of intraocular pressure without cls and with + 2.00  d and + 6.00  d was 19.0    4.1  mmhg ( range : 9.027.5 ) , 17.6    4.6  mmhg ( range : 10.525.0 ) and 17.8    4.1  mmhg ( range : 10.824.8 ) respectively ( fig . the correlations between iop measurements without cls and with the positive cls were  >  0.9 ( p  <  0.05 in all cases ) . altman plots for the comparison between the measurement with + 2.00  d cl and without cl ( rt ) and the measurement with + 6.00  d cl and without cl respectively are shown in figure 2 , figure 3 . 2 ) shows that there is no proportional bias : no significant trend was detected for differences between + 2.00  d and rt measurements as a function of their mean value ( r  =  0.30 , p  =  0.141 ) . 3 ) for the + 6.00  d and rt measurements gave a similar result , mean value ( r  = 0.006 , p  =  0.98 ) . table 3 gives the paired - samples t - test between the measurements with and without cls . all the comparisons between the measurements without cls and the measurements with the positive cls were significant . the comparison between measures obtained with the + 2.00  d and + 6.00  d cls was not significant . in order to evaluate if corneal parameters such as thickness , curvature or asphericity were affecting the difference in iop measurement with and without cls , a coefficient of correlation was calculated . none of these parameters correlated with the difference between iop values obtained with + 2.00  d and rt or + 6.00  d and rt . iop measurement with a rebound tonometer over positive hydrogel cls provides statistically significant lower values than the measurement without cls . the decrease is not proportional to the increase in refractive power of the cl . despite the fact the difference is statistically significant , from a clinical point of view this difference is minimal because it is almost at the cut off value of more than 1.5  mmhg that is considered relevant . although the rebound tonometer is among the most recent instruments used today , it is gaining a relative acceptance especially for the simplicity of the procedure and that topical anaesthesia and fluorescein are not required . the results are reproducible and reasonably accurate.32 , 33 furthermore it can be used in challenging patients such as children51 , 52 or the disabled . it has been shown that with the rebound tonometer it is possible to measure iop over soft cls , either hydrogel ( etafilcon a ) or silicone hydrogel ( senofilcon a ) , with good accuracy . in this study the underestimation of iop was greater for power + 2.00  d compared to cls of negative power . our results appear to conflict with previous studies where the tonometry measurement was taken using conventional applanation tonometers , especially the air puff type , where positive soft cls contribute to an increase in the value of iop.7 , 20 , 21 , 22 , 23 , 54 , 55 , 56 the results from tonometry can be influenced by the characteristics of the patient 's cornea such as corneal thickness57 , 58 corneal curvature and corneal biomechanical factors . true iop will be overestimated in eyes with thick corneas , a steep corneal curvature and high corneal hysteresis . however , most researchers have considered the effect of tonometry based on applanation principles . regarding the rebound tonometer , chui et al . , have found that the result is affected by biomechanical corneal properties but not corneal thickness . jorge et al . , found that although corneal thickness can play a role in rebound tonometry , individual physiological variations of biomechanical corneal properties such as the elastic and viscoelastic responses , may be more relevant factors . in a recent cross - sectional study , the effect of plano power lotrafilcon a contact lenses in situ on iop measurement from three portable tonometers , including icare , a statistically significant overestimation of 1.00  mmhg was found between iop measurements obtained with the rebound tonometer with and without cls . this result could be attributed to the higher modulus of lotrafilcon a which should offer more resistance to the deformation than cls in senofilcon a ( used in the previous study ) , and etafilcon a ( used in the previous and present studies ) . a similar increment in iop was found by anton et al . , when measuring iop with a rebound tonometer over a silicone hydrogel soft cl ( balafilcon a ) characterized by a water content of 36% , back vertex power of plano and a central thickness of 0.07  mm . the decrement in iop found in the present study could be attributed to low resistance to deformation produced by etafilcon a a hydrogel characterized by high water content . in this study , we have had the opportunity to compare the effect on iop induced by two cls having positive powers , and significantly different thickness . the presence of the same trend in the change of iop induced by the two cls leads us to believe , in accordance with chui et al . , that the thickness at the centre of the cornea , or cornea together with the cl , does not affect the value of iop assessed with a rebound tonometer . in conclusion , the measurement of iop while positive power cls having a water content of 58% , such as those in etafilcon a , are worn , tends to give lower values than those obtained without cls . eye care practitioners should keep this in mind when analyzing iop values or remove positive cls before performing rebound tonometry measurements .
purposeto investigate if the accuracy of intraocular pressure ( iop ) measurements using rebound tonometry over disposable hydrogel ( etafilcon a ) contact lenses ( cl ) is affected by the positive power of the cls.methodsthe experimental group comprised 26 subjects , ( 8 male , 18 female ) . 
 iop measurements were undertaken on the subjects right eyes in random order using a rebound tonometer ( icare ) . 
 the cls had powers of + 2.00  d and + 6.00  d. measurements were taken over each contact lens and also before and after the cls had been worn.resultsthe iop measure obtained with both cls was significantly lower compared to the value without cls ( t test ; p  <  0.001 ) but no significant difference was found between the two powers of cls.conclusionsrebound tonometry over positive hydrogel cls leads to a certain degree of iop underestimation . 
 this result did not change for the two positive lenses used in the experiment , despite their large difference in power and therefore in lens thickness . 
 optometrists should bear this in mind when measuring iop with the rebound tonometer over plus power contact lenses .