a 72-year - old man presented with a two - week history of a red painful right eye . he was a soft contact lens wearer and wore his lenses for one month at a time , taking them out at the end of each day and disposing of the lenses at the end of the month . he had no past ocular , medical , or drug history of note . on examination he had an inflamed eye with a central corneal epithelial defect 3.0 mm  3.5 mm in size , with surrounding superficial and midstromal infiltration . a corneal scrape of the right eye was performed for gram staining and cultures were obtained on blood , chocolate , and sabouraud s agar , as well as non - nutrient agar overlaid with escherichia coli . he was started on empirical antimicrobial treatment with topical ofloxacin hourly by day and night . there was no growth of any other organisms , and the topical ofloxacin was discontinued . his treatment was switched to topical , ie , hourly polyhexamethylene biguanide 0.02% , hourly propamidine isethionate 0.1% ( brolene ) , prednisolone 0.5% four times daily , and atropine 1% three times daily . over the following three months , his treatment was slowly tapered to topical , ie , polyhexamethylene biguanide four times daily , brolene four times daily , and prednisolone 0.1% twice daily as his clinical picture improved . at three months after initial diagnosis he complained of the right eye being red and sore again for several days . on examination the gram stain showed gram - positive cocci in chains and he was empirically commenced on topical penicillin hourly , which was added to his prophylactic treatment for acanthamoeba . after 48 hours , the blood agar grew streptococcus viridans which was shown to be sensitive to penicillin . topical penicillin was tapered down to twice daily as his condition improved over the following month . four months after initial diagnosis , he again complained of a red painful right eye for one week . on examination fine debris and hairs were noted in the base of the abscess and were removed by scraping ( figure 1 ) . the penicillin drops were stopped and he was treated with topical ofloxacin initially hourly and then tapered off over one month . five months after initial diagnosis he complained of a one - day history of a painful right eye . fine strands were noted on the surface of a central corneal abscess and were removed during the corneal scrape . he was treated with topical ofloxacin and gentamicin , to which the organism was found to be sensitive . the organism was resistant to ciprofloxacin , chloramphenicol , penicillin , cefuroxime , and fusidic acid . as he improved , fine fibrils were again seen in the healing cornea and were removed and sent for microscopy . this confirmed that the fibrils were synthetic and were not observed in previous / present cultures , and presumed to be derived from tissues which the patient was constantly using to wipe his eye . the cornea continued to heal and topical treatment for acanthamoeba ( including steroids and ofloxacin ) were continued and tailed off over a further three months . in all episodes of infection , antibiotic sensitivities were determined using disc diffusion susceptibility testing . in all episodes of reinfection , scraping for acanthamoeba was performed to exclude reactivation of the initial infection . this patient had acanthamoeba keratitis secondary to contact lens wear and then developed three episodes of bacterial keratitis . microbial keratitis results from the interaction of a broad spectrum of pathogens and a diverse range of host responses . recurrence is rare in the absence of predisposing factors , such as contact lens wear , ocular surface and corneal disease , corneal anesthesia , exposure , trauma , or previous corneal surgery.1 when suspected , corneal scraping is mandatory in order to provide material for a microbiological diagnosis , debride necrotic tissue , and enhance antibiotic penetration . corneal biopsy may be necessary in some cases of recurrence . the initial bacterial infection in this patient presumably was facilitated by the underlying acanthamoeba keratitis and the use of corticosteroids.2,3 the presence of synthetic fibrils on the ulcer base presumably contributed to the infection recurrences by acting as a nidus for organisms and by interfering with corneal healing . it is noteworthy that the cornea finally healed with no further infections once the patient was instructed not to wipe his eye with a paper tissue . unfortunately it was not possible to obtain cultures directly from the tissue fibrils to confirm their direct association with the infective organisms . the first two bacterial infections were due to virulent organisms , such as pseudomonas and streptococcus . the culture of stenotrophomonas maltophilia from the third corneal abscess was feasible because this is an opportunistic organism and infections typically occur in patients with compromised ocular surface and trauma.4,5 it has been reported previously in patients following penetrating keratoplasty.3,6,7 this is the first case report of acanthamoeba keratitis occurring in a contact lens wearer with preceding protozoal and bacterial keratitis . the characteristically resistant antibiogram of s. maltophilia may limit the therapeutic options . fortunately in this patient treatment
recurrence of microbial keratitis in the presence of protozoal infection is very rare and infrequently reported unless predisposing factors are present . 
 the association of recurrent microbial keratitis and synthetic microfibrils has never previously been reported to our knowledge . 
 this single interventional case study describes the clinical course and treatment of a contact lens wearer who was treated for acanthamoeba keratitis with superinfection from bacterial organisms in the presence of synthetic microfibrils . 
 the presence of synthetic fibrils on a corneal ulcer base may act as a nidus for pathological organisms and interfere with normal corneal healing . 
 this may result in infection recurrence and the growth of resistant opportunistic organisms .