proteomics aims to identify large sets of proteins in defined biological fractions , e.g. , tissue or cell extracts , isolated organelles , or protein fractions generated by biochemical preparation procedures . as a first step , protein identifications today are nearly exclusively carried out by mass spectrometry ( ms ) . however , since the mass of a native protein normally does not allow any conclusion on its identity , proteins have to be fragmented by specific endopeptidases before mass analyses to generate defined peptides . these peptide fragments are then searched against a database of theoretically digested proteins using a software package such as mascot , sequest , or x ! many proteome projects use one- or two - dimensional ( 2d ) gel electrophoresis for protein separation . following this experimental strategy , proteins are first separated , then individually  picked  from gels , treated with an endopeptidase ( trypsin ) and finally identified by ms and database interrogation . as a result ,  proteome reference maps proteome maps were developed in several fields of biology and are greatly appreciated by the scientific community ( see for instance gallardo et al . originally , maps were exclusively presented in scientific publications in the form of images and long lists of identified proteins which are assigned to the spots on the image using arrows and numbers . about a decade ago , the first web - based resources were developed which offer interactive features in the maps : upon  clicking  on a spot , information on a protein is presented in a small pop - up window which sometimes is linked to further background information in databases ( examples at http://seed.proteome.free.fr/ , http://gabi.rzpd.de/projects/arabidopsis_proteomics/ , http://gene64.dna.affrc.go.jp/rpd/ ) . recently , the gelmap software package for annotating gel - based proteome data was developed . it offers several new possibilities for the web - based publication of reference maps , e.g. , functional annotation of proteins and assignment of multiple proteins to individual  spots  on a gel ( rode et al . , 2011a ) . in the following sections , we summarize the characteristics of gelmap and introduce novel features which were newly implemented into the gelmap software package ( gelmap 2.0 ) . gelmap is offered as a web tool free of charge at www.gelmap.de . minimal requirement for building a new map are ( a ) an image file and ( b ) a protein table which includes the coordinates of the spots on the gel . ( by reading the spot s coordinates from graphics software like photoshop , gimp , or even ms paint ) but is usually generated automatically by specialized proteomics software tools like delta2d ( decodon , greifswald , germany ) , decyder ( ge healthcare , munich , germany ) , or progenesis samespots ( non - linear dynamics , newcastle , uk ) . to use gelmap to full capacity , the table should be extended with information on ( 1 ) the spot identification number ( corresponding to the number on the gel image ) , ( 2 ) the mascot probability score ( or the score of another matching software package ) , ( 3 ) coverage of a protein by identified peptides , ( 4 ) the accession number of a protein , ( 5 ) the database used for protein identification , ( 6 ) assignment of a protein to functional categories like a protein complex , a physiological area or subcellular localization , ( 7 ) molecular mass ( calculated and/or apparent mass on the gel ) , etc . ( table 1 ) . to ensure that gelmap recognizes ids , coordinates , scores , accession numbers , and filters , a second line beneath the line with the column names has to include special keywords ( table 1 ) . the third line can contain commentaries for the tooltips in gelmaps spreadsheet view ( figure 1 ) . to mark the columns of high importance since gelmap provides a three - level filter tree , the fifth line can define if this column should be used as a ( 1 ) root level , ( 2 ) second level , or ( 3 ) third level filter . this control information is required for a fully functional map and is documented with examples on www.gelmap.de/howto . the two icons given in the header allow access to the  protein spreadsheet  and the  peptide spreadsheet . the image file and the  protein spreadsheet  file can be directly uploaded at www.gelmap.de/create . the resulting map consists of the gel image , a side menu for functional categories of the identified proteins ( right side ) , and frames for search options ( figure 1 ) . , information automatically is displayed in the following way : ( i ) upon hovering over a spot , all proteins identified within this spot are displayed within a tooltip . proteins are ordered according to their mascot probability scores ( this , on a semi - quantitative level , reflects abundance of the identified proteins within a protein spot ) . ( ii ) after clicking a spot , the protein names included in the tooltip are transformed into links , which can be used to get protein - specific information within a second info frame . in addition to the information on the protein accession and the calculated molecular mass of the protein this frame can offer links to an external database ( via the accession number ) and to the  protein spreadsheet  ( link  more protein details  ) . the  protein spreadsheet  also can be accessed directly by using an icon in the header ( figure 1 ) . ( iii ) proteins assigned to functional categories are accessible via the three - level tree in the side menu . upon clicking onto a topic included in this menu , all proteins forming part of this category automatically become highlighted by circles on the gel image . at the same time , subcategories become visible for many topics of the main menu , which allow to differentially visualize proteins assigned to the next functional level . for details we recommend visiting one of the established gelmaps , e.g. , http://gelmap.de/47 . to display primary ms data for all identified proteins , a second table called  peptide spreadsheet  was introduced in gelmap 2.0 ( figure 1 ) . this table may include information on all identified peptides within a spot . in the case of the arabidopsis thaliana bn / sds map , the table includes information on the amino acid sequences of the peptides , peptide modifications , and positions of peptides with respect to the complete protein sequences ( column  range  : number of the first and the last amino acid of a peptide with respect to the n - terminal methionine ) . meta portals  which are realized on the basis of peptide position data ( see integration of gelmap into the mascp gator ) . the linked icon for the  peptide spreadsheet  is located next to the  protein spreadsheet  icon at the top ( figure 1 ) . alternatively , the  peptide spreadsheet  is accessible through a text link in the information frames for individual proteins on the gel image . the content of this table can be freely defined as long as the first column contains the hit s i d to link this information to the spots ( see http://www.gelmap.de/47 for example ) . in summary , gelmap offers the following features : proteins of interest can easily be found using the different search options or by browsing / navigating through / using the categories in the side menu . since all proteins are annotated according to functional criteria , sets of proteins involved in metabolic processes or other functional categories are easily visualized . detailed information on the identified proteins is provided by the information frames which are accessible via the gel image . background information and raw data on all proteins is given in the  protein spreadsheet  and the  peptide spreadsheet gelmap allows complete annotation of ms data for all analyzed protein spots : not only the protein with the highest mascot probability score is given , but all identified proteins above a lower boundary score to be defined by the user . the gelmap portal was established for creation and presentation of reference maps . upon finishing a new map , a user can decide to make it accessible via the official gelmap site and/or via a direct link which can be presented in publications . gelmap also allows private ( password protected ) projects to share proteome data in a closed group or analyzing results without sharing them . currently ( february 28th , 2012 ) , four projects are publicly available at the gelmap portal : the cyclamen persicum seed proteome ( rode et al . , 2011b ) . in the frame of this project , identified proteins are assigned to 30 different metabolic pathways within 10 metabolic divisions . furthermore . in addition to the features mentioned above , this gelmap allows to differentially display proteins of changed abundance between the two compared protein fractions . this illustrates the various extensions which easily can be realized based on the gelmap software package . the a. thaliana mitochondrial proteome separated by 2d blue native / sds page ( klodmann et al . , 2011 this map is based on a special protein separation procedure : protein complexes are separated under native conditions on the first gel dimension and the subunits of the protein complexes under denaturing conditions on the second dimension , which is carried out in the presence of sds . on the resulting gels , proteins belonging to the same protein complex form a vertical row of spots . for 2d blue native / sds page , gelmap offers special advantages : selective display of functional categories allows identifying vertical positioning of proteins of low abundance . this led to the discovery of new protein complexes in arabidopsis mitochondria . in the frame of this study , 471 non - redundant proteins were identified and assigned to more than 35 protein complexes . the a. thaliana mitochondrial proteome separated by 2d ief / sds page ( taylor et al . , 2011 ) . recently , the proteomic aggregation utility  mascp gator  has been established to link proteome databases for the model plant a. thaliana ( joshi et al . , 2011 ) . in this platform , protein data is simultaneous displayed from many different international repositories to allow assessment of the global knowledge of a protein or several proteins . so far , projects integrated in the mascp gator exclusively represented shot - gun approaches not based on 2d page . since september 2011 , gelmap offers a limited application programming interface (  api  ) which allows other projects ( such as gator ) to run automated search queries on the gelmap database and collect the results . the two gelmaps dedicated to the mitochondrial proteome of arabidopsis very recently were the first gel - based projects integrated into the mascp gator . upon submission of a protein accession , the user of the mascp gator directly can access the reference maps at www.gelmap.de ( figure 2 ) . information on peptides available at gelmap is graphically displayed at the mascp gator site together with peptide information of other databases . in the future integration of the mitochondrial reference maps at gelmap into mascp gator ( http://gator.masc-proteomics.org/ ) . upon submission of a protein accession ( here : at2g47260 ) , matching peptides from gelmap are graphically displayed at the gator site . direct access to gelmap is provided by links in the left side menu of the gator . the gelmap software package allows complete annotation of ms data corresponding to 2d protein separations . based on its central features  functional annotation of proteins and at the same time annotation of complete sets of proteins identified within each spot  it allows the comprehensive evaluation of gel - based proteome data sets . in the future , the gelmap platform will be used to annotate further projects . integration of these projects into the mascp gator might soon allow extensive coverage of the arabidopsis proteome by gel - based analyses . the authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest .
in classical proteome analyses , final experimental data are ( a ) images of 2d protein separations obtained by gel electrophoresis and ( b ) corresponding lists of proteins which were identified by mass spectrometry ( ms ) . for data 
 annotation , software tools were developed which allow the linking of protein identity data directly to 2d gels (  clickable gels  ) . 
 gelmap is a new online software tool to annotate 2d protein maps . 
 it allows ( i ) functional annotation of all identified proteins according to biological categories defined by the user , e.g. , subcellular localization , metabolic pathway , or assignment to a protein complex and ( ii ) annotation of several proteins per analyzed protein  spot  according to ms primary data . 
 options to differentially display proteins of functional categories offer new opportunities for data evaluation . 
 for instance , if used for the annotation of 2d blue native / sds gels , gelmap allows the identification of protein complexes of low abundance . 
 a web portal has been established for presentation and evaluation of protein identity data related to 2d gels and is freely accessible at http://www.gelmap.de/.