Mutilene (kwa Kigiriki: Μυτιλήνη, Mytilíni) ni mji wa Ugiriki, makao makuu ya kisiwa cha Lesbo katika Bahari ya Aegean chenye eneo la Km² 1,633. Una wakazi 29,656 (2011) wanaotegemea zaidi uvuvi na utalii. Mwaka 58 Mtume Paulo, katika safari yake ya tatu ya kimisionari, aliabiri kutoka huko hadi Samos akielekea Yerusalemu. Mdo 20:14 And when he met us at Assos, we took him on board and came to Mitylene. 15 We sailed from there, and the next day came opposite Chios. The following day we arrived at Samos and stayed at Trogyllium. The next day we came to Miletus. 16 For Paul had decided to sail past Ephesus, so that he would not have to spend time in Asia; for he was hurrying to be at Jerusalem, if possible, on the Day of Pentecost. Wikimedia Commons ina media kuhusu: Mutilene Official website Calendar of Lesvos Tourist Guide of Mytilene-Lesvos