Khamis Gaddafi (An haife shi ne a ranar a 27 ga watan Mayun 1983 - 29 August 2011) shi ne na bakwai kuma ƙarami a ɗa ga tsohon shugaban Libya Muammar Gaddafi, kuma kwamandan soja mai kula da Khamis Brigade na Sojojin Libya . Ya kasance wani ɓangare na mahaifinsa na ciki. A lokacin yakin basasar Libya a shekara ta 2011, ya kasance wata babbar manufa ga sojojin adawa da ke kokarin kifar da mahaifinsa. Yana dan shekara uku, Khamis Gaddafi ya ji rauni a harin bam din Amurka na 15 ga watan Afrilu 1986 a Libya, yana fama da rauni a kansa lokacin da aka kai hari kan sansanin soja na Bab al-Azizia a matsayin ramuwar gayya game da harin bam din na 1986 a Berlin. Ya kammala karatunsa a makarantar sojan da ke Tripoli, inda ya samu digiri na farko a fannin kere kere da kimiyya, sannan ya ci gaba da karatunsa a Kwalejin Soja ta Frunze da ke Moscow da Kwalejin Soja ta Janar na Sojojin Rasha . A shekarar 2008, Gaddafi ya ziyarci Algeria, inda Shugaba Abdelaziz Bouteflika ya tarbe shi .